LOCUS       DADLFA010000001        77590 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000001 DADLFA010000000
VERSION     DADLFA010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 77590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77590
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.001"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..1624
                     /locus_tag="JQH65_000001"
     CDS             <1..1624
                     /locus_tag="JQH65_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY6137536.1"
                     /translation="NNGSQVNVTDSSLVSDTIGLTNGSSLNIGANGVVATDHLTVDSY
                     STVNLTESTGWNNYSNLYTNTITVTNGGVLDVNVDQFDTEAFRTDKLELTSGNIADHN
                     GNVISGVFDINSSDYVLNADLVNDRTWDTSKSNYGYGIVAMNSDGHLTINGNGDVDNG
                     TELDNSSVDNVVAATGNYKVRIDNATGAGTIADYKDKELIRVNDANTNATFSAANKAD
                     LGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNLEQDTVGTRLTNSRHGL
                     ADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGD
                     MNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNS
                     DAWGFGLKAGYDFKLGDAGYETPYGSVSGLFQSGDDYQLSNDMKVDGQSYDSMRYELG
                     VDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSF
                     TKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
     gene            1712..2335
                     /gene="iprA"
                     /locus_tag="JQH65_000002"
     CDS             1712..2335
                     /gene="iprA"
                     /locus_tag="JQH65_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HAY6137537.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNSEDT
                     FFILEGVISLRREENVLIGITQASYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINCLPSEY"
     gene            complement(2336..3493)
                     /locus_tag="JQH65_000003"
     CDS             complement(2336..3493)
                     /locus_tag="JQH65_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-12 family class D beta-lactamase AmpH"
                     /protein_id="HAY6137538.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            3845..5065
                     /gene="sbmA"
                     /locus_tag="JQH65_000004"
     CDS             3845..5065
                     /gene="sbmA"
                     /locus_tag="JQH65_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HAY6137539.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLVA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            5078..6172
                     /gene="yaiW"
                     /locus_tag="JQH65_000005"
     CDS             5078..6172
                     /gene="yaiW"
                     /locus_tag="JQH65_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HAY6137540.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(6231..6539)
                     /locus_tag="JQH65_000006"
     CDS             complement(6231..6539)
                     /locus_tag="JQH65_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2755 family protein"
                     /protein_id="HAY6137541.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            6799..7011
                     /locus_tag="JQH65_000007"
     CDS             6799..7011
                     /locus_tag="JQH65_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HAY6137542.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            complement(7035..8129)
                     /gene="ddlA"
                     /locus_tag="JQH65_000008"
     CDS             complement(7035..8129)
                     /gene="ddlA"
                     /locus_tag="JQH65_000008"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAY6137543.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPSKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLRLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            8207..8364
                     /locus_tag="JQH65_000009"
                     /pseudo
     CDS             8207..8364
                     /locus_tag="JQH65_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8591..8851
                     /gene="iraP"
                     /locus_tag="JQH65_000010"
     CDS             8591..8851
                     /gene="iraP"
                     /locus_tag="JQH65_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HAY6137544.1"
                     /translation="MKNLIAELLLKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIEQVEGALYEVKPDASIPDDDTELLRNYVKKLLKHPRQ"
     gene            8952..10367
                     /gene="phoA"
                     /locus_tag="JQH65_000011"
     CDS             8952..10367
                     /gene="phoA"
                     /locus_tag="JQH65_000011"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HAY6137545.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNAVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            10486..10806
                     /gene="psiF"
                     /locus_tag="JQH65_000012"
     CDS             10486..10806
                     /gene="psiF"
                     /locus_tag="JQH65_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HAY6137546.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            10908..12023
                     /gene="adrA"
                     /locus_tag="JQH65_000013"
                     /gene_synonym="dgcC"
     CDS             10908..12023
                     /gene="adrA"
                     /locus_tag="JQH65_000013"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HAY6137547.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(12040..12849)
                     /gene="proC"
                     /locus_tag="JQH65_000014"
     CDS             complement(12040..12849)
                     /gene="proC"
                     /locus_tag="JQH65_000014"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAY6137548.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPNKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            12969..13427
                     /locus_tag="JQH65_000015"
     CDS             12969..13427
                     /locus_tag="JQH65_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAY6137549.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            13610..14134
                     /gene="aroL"
                     /locus_tag="JQH65_000016"
     CDS             13610..14134
                     /gene="aroL"
                     /locus_tag="JQH65_000016"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HAY6137550.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            14184..14375
                     /gene="yaiA"
                     /locus_tag="JQH65_000017"
     CDS             14184..14375
                     /gene="yaiA"
                     /locus_tag="JQH65_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HAY6137551.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            14633..15310
                     /locus_tag="JQH65_000018"
     CDS             14633..15310
                     /locus_tag="JQH65_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HAY6137552.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            15382..15666
                     /gene="ppnP"
                     /locus_tag="JQH65_000019"
     CDS             15382..15666
                     /gene="ppnP"
                     /locus_tag="JQH65_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HAY6137553.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            15814..16155
                     /locus_tag="JQH65_000020"
                     /pseudo
     CDS             15814..16155
                     /locus_tag="JQH65_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            16152..16235
                     /locus_tag="JQH65_000021"
     CDS             16152..16235
                     /locus_tag="JQH65_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6137554.1"
                     /translation="MTQRPWSKLQHKTHNIAALKIIVRRSE"
     gene            complement(16313..17224)
                     /gene="rdgC"
                     /locus_tag="JQH65_000022"
     CDS             complement(16313..17224)
                     /gene="rdgC"
                     /locus_tag="JQH65_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HAY6137555.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            17349..18258
                     /gene="mak"
                     /locus_tag="JQH65_000023"
                     /pseudo
     CDS             17349..18258
                     /gene="mak"
                     /locus_tag="JQH65_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
     gene            complement(18401..19585)
                     /gene="araJ"
                     /locus_tag="JQH65_000024"
     CDS             complement(18401..19585)
                     /gene="araJ"
                     /locus_tag="JQH65_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HAY6137556.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMFFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADTPVLAKP
                     LG"
     gene            complement(19711..22854)
                     /gene="sbcC"
                     /locus_tag="JQH65_000025"
     CDS             complement(19711..22854)
                     /gene="sbcC"
                     /locus_tag="JQH65_000025"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HAY6137557.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NGSLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVKAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHKRQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTTLQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            complement(22851..24053)
                     /gene="sbcD"
                     /locus_tag="JQH65_000026"
     CDS             complement(22851..24053)
                     /gene="sbcD"
                     /locus_tag="JQH65_000026"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HAY6137558.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALAESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            24243..24932
                     /gene="phoB"
                     /locus_tag="JQH65_000027"
     CDS             24243..24932
                     /gene="phoB"
                     /locus_tag="JQH65_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HAY6137559.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            24990..26285
                     /gene="phoR"
                     /locus_tag="JQH65_000028"
     CDS             24990..26285
                     /gene="phoR"
                     /locus_tag="JQH65_000028"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HAY6137560.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            26692..28011
                     /gene="brnQ"
                     /locus_tag="JQH65_000029"
     CDS             26692..28011
                     /gene="brnQ"
                     /locus_tag="JQH65_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HAY6137561.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            28087..29460
                     /gene="proY"
                     /locus_tag="JQH65_000030"
     CDS             28087..29460
                     /gene="proY"
                     /locus_tag="JQH65_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HAY6137562.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            29616..31338
                     /gene="malZ"
                     /locus_tag="JQH65_000031"
                     /pseudo
     CDS             29616..31338
                     /gene="malZ"
                     /locus_tag="JQH65_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
     gene            complement(31343..31924)
                     /gene="acpH"
                     /locus_tag="JQH65_000032"
     CDS             complement(31343..31924)
                     /gene="acpH"
                     /locus_tag="JQH65_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HAY6137563.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRRETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFI
                     CYARKQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLNAHYTALGTRFWQFYPRMMAQASHKAL"
     gene            32017..33087
                     /gene="queA"
                     /locus_tag="JQH65_000033"
     CDS             32017..33087
                     /gene="queA"
                     /locus_tag="JQH65_000033"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAY6137564.1"
                     /translation="MRVTDFFFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDDRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            33143..34270
                     /gene="tgt"
                     /locus_tag="JQH65_000034"
     CDS             33143..34270
                     /gene="tgt"
                     /locus_tag="JQH65_000034"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAY6137565.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            34293..34625
                     /gene="yajC"
                     /locus_tag="JQH65_000035"
     CDS             34293..34625
                     /gene="yajC"
                     /locus_tag="JQH65_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAY6137566.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            34653..36500
                     /gene="secD"
                     /locus_tag="JQH65_000036"
     CDS             34653..36500
                     /gene="secD"
                     /locus_tag="JQH65_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HAY6137567.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            36511..37482
                     /gene="secF"
                     /locus_tag="JQH65_000037"
     CDS             36511..37482
                     /gene="secF"
                     /locus_tag="JQH65_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HAY6137568.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLTQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            37611..37958
                     /locus_tag="JQH65_000038"
     CDS             37611..37958
                     /locus_tag="JQH65_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HAY6137569.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(38135..39019)
                     /locus_tag="JQH65_000039"
     CDS             complement(38135..39019)
                     /locus_tag="JQH65_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HAY6137570.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLNFGPFKEWYFANNYIYDMGRNKNGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(39318..39857)
                     /locus_tag="JQH65_000040"
     CDS             complement(39318..39857)
                     /locus_tag="JQH65_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HAY6137571.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGGYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            40008..40457
                     /gene="nrdR"
                     /locus_tag="JQH65_000041"
     CDS             40008..40457
                     /gene="nrdR"
                     /locus_tag="JQH65_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HAY6137572.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            40461..41564
                     /gene="ribD"
                     /locus_tag="JQH65_000042"
     CDS             40461..41564
                     /gene="ribD"
                     /locus_tag="JQH65_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HAY6137573.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHHIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWGEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            41653..42123
                     /gene="ribE"
                     /locus_tag="JQH65_000043"
     CDS             41653..42123
                     /gene="ribE"
                     /locus_tag="JQH65_000043"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HAY6137574.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            42143..42562
                     /gene="nusB"
                     /locus_tag="JQH65_000044"
     CDS             42143..42562
                     /gene="nusB"
                     /locus_tag="JQH65_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAY6137575.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            42640..43617
                     /gene="thiL"
                     /locus_tag="JQH65_000045"
     CDS             42640..43617
                     /gene="thiL"
                     /locus_tag="JQH65_000045"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HAY6137576.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGVRIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            43595..44113
                     /gene="pgpA"
                     /locus_tag="JQH65_000046"
     CDS             43595..44113
                     /gene="pgpA"
                     /locus_tag="JQH65_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAY6137577.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(44167..45141)
                     /gene="yajO"
                     /locus_tag="JQH65_000047"
     CDS             complement(44167..45141)
                     /gene="yajO"
                     /locus_tag="JQH65_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HAY6137578.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLFYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(45196..47058)
                     /gene="dxs"
                     /locus_tag="JQH65_000048"
     CDS             complement(45196..47058)
                     /gene="dxs"
                     /locus_tag="JQH65_000048"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAY6137579.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            complement(47083..47982)
                     /gene="ispA"
                     /locus_tag="JQH65_000049"
     CDS             complement(47083..47982)
                     /gene="ispA"
                     /locus_tag="JQH65_000049"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HAY6137580.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSPDTSALEALADYIIQRNK"
     gene            complement(47982..48224)
                     /gene="xseB"
                     /locus_tag="JQH65_000050"
     CDS             complement(47982..48224)
                     /gene="xseB"
                     /locus_tag="JQH65_000050"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015571292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAY6137581.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            48430..49878
                     /gene="thiI"
                     /locus_tag="JQH65_000051"
     CDS             48430..49878
                     /gene="thiI"
                     /locus_tag="JQH65_000051"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAY6137582.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIALQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            complement(49932..50522)
                     /gene="yajL"
                     /locus_tag="JQH65_000052"
     CDS             complement(49932..50522)
                     /gene="yajL"
                     /locus_tag="JQH65_000052"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HAY6137583.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLQVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(50485..51396)
                     /gene="panE"
                     /locus_tag="JQH65_000053"
     CDS             complement(50485..51396)
                     /gene="panE"
                     /locus_tag="JQH65_000053"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAY6137584.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSANLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            51564..52055
                     /gene="yajQ"
                     /locus_tag="JQH65_000054"
     CDS             51564..52055
                     /gene="yajQ"
                     /locus_tag="JQH65_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HAY6137585.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            complement(52183..53547)
                     /locus_tag="JQH65_000055"
     CDS             complement(52183..53547)
                     /locus_tag="JQH65_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY6137586.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPA
                     YVSSLRIEIPANIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFDVEQA
                     IRQA"
     gene            complement(53696..54586)
                     /gene="cyoE"
                     /locus_tag="JQH65_000056"
     CDS             complement(53696..54586)
                     /gene="cyoE"
                     /locus_tag="JQH65_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HAY6137587.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(54598..54927)
                     /locus_tag="JQH65_000057"
     CDS             complement(54598..54927)
                     /locus_tag="JQH65_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HAY6137588.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(54927..55541)
                     /locus_tag="JQH65_000058"
     CDS             complement(54927..55541)
                     /locus_tag="JQH65_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HAY6137589.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTCLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(55531..57522)
                     /gene="cyoB"
                     /locus_tag="JQH65_000059"
     CDS             complement(55531..57522)
                     /gene="cyoB"
                     /locus_tag="JQH65_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HAY6137590.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWANSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(57544..58491)
                     /gene="cyoA"
                     /locus_tag="JQH65_000060"
     CDS             complement(57544..58491)
                     /gene="cyoA"
                     /locus_tag="JQH65_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HAY6137591.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            complement(58952..60427)
                     /gene="ampG"
                     /locus_tag="JQH65_000061"
     CDS             complement(58952..60427)
                     /gene="ampG"
                     /locus_tag="JQH65_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HAY6137592.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(60471..61049)
                     /locus_tag="JQH65_000062"
     CDS             complement(60471..61049)
                     /locus_tag="JQH65_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY6137593.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLAASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKITKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            61354..61671
                     /gene="bolA"
                     /locus_tag="JQH65_000063"
     CDS             61354..61671
                     /gene="bolA"
                     /locus_tag="JQH65_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HAY6137594.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            62015..63313
                     /gene="tig"
                     /locus_tag="JQH65_000064"
     CDS             62015..63313
                     /gene="tig"
                     /locus_tag="JQH65_000064"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAY6137595.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDSAVEA
                     EDRVTIDFTGSVDSEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            63558..64181
                     /gene="clpP"
                     /locus_tag="JQH65_000065"
     CDS             63558..64181
                     /gene="clpP"
                     /locus_tag="JQH65_000065"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAY6137596.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            64307..65581
                     /gene="clpX"
                     /locus_tag="JQH65_000066"
     CDS             64307..65581
                     /gene="clpX"
                     /locus_tag="JQH65_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HAY6137597.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            65769..68123
                     /gene="lon"
                     /locus_tag="JQH65_000067"
     CDS             65769..68123
                     /gene="lon"
                     /locus_tag="JQH65_000067"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HAY6137598.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENCVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            68332..68604
                     /gene="hupB"
                     /locus_tag="JQH65_000068"
     CDS             68332..68604
                     /gene="hupB"
                     /locus_tag="JQH65_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HAY6137599.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            68796..70667
                     /gene="ppiD"
                     /locus_tag="JQH65_000069"
     CDS             68796..70667
                     /gene="ppiD"
                     /locus_tag="JQH65_000069"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAY6137600.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFAQPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            70818..71189
                     /locus_tag="JQH65_000070"
     CDS             70818..71189
                     /locus_tag="JQH65_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6137601.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            71283..71681
                     /locus_tag="JQH65_000071"
     CDS             71283..71681
                     /locus_tag="JQH65_000071"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HAY6137602.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(71733..72428)
                     /gene="queC"
                     /locus_tag="JQH65_000072"
     CDS             complement(71733..72428)
                     /gene="queC"
                     /locus_tag="JQH65_000072"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HAY6137603.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(72493..74193)
                     /locus_tag="JQH65_000073"
     CDS             complement(72493..74193)
                     /locus_tag="JQH65_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HAY6137604.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            74293..75075
                     /gene="cof"
                     /locus_tag="JQH65_000074"
     CDS             74293..75075
                     /gene="cof"
                     /locus_tag="JQH65_000074"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HAY6137605.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTLVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTH"
     gene            75177..75722
                     /locus_tag="JQH65_000075"
     CDS             75177..75722
                     /locus_tag="JQH65_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HAY6137606.1"
                     /translation="MVFKPHRINCRDFTFKIELKEKKILCGRAMLDKIDRKLLALLQQ
                     DCTLSLQALAEAVNLTTTPCWKRLKRLEDDGILIGKVALLDPEKIGLGLTAFVLIKTQ
                     HHSSEWYCRFVTVVTEMPEVLGFWRMAGEYDYLMRVQVADMKRYDEFYKRLVNSVPGL
                     SDVTSSFAMEQIKYTTSLPIE"
     gene            75752..77413
                     /locus_tag="JQH65_000076"
                     /pseudo
     CDS             75752..77413
                     /locus_tag="JQH65_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235587.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
BASE COUNT        18961 a        19226 c        20624 g        18779 t
ORIGIN      
        1 aaataatggc tctcaggtaa atgttactga ttcctctctg gtatcagata ccattggttt
       61 aaccaacggt tcttcactga atattggtgc aaatggcgtg gttgctacag accacctgac
      121 tgttgatagc tacagtaccg ttaatttaac cgaatctacc ggttggaata attattctaa
      181 tctgtatacc aatactatca ctgtgactaa cggtggtgtg ctggatgtga atgttgatca
      241 gttcgatact gaagctttcc gtactgataa actggaactg accagcggca acattgctga
      301 ccataatggc aacgtaattt ctggtgtgtt cgacatcaac agcagcgatt acgttctgaa
      361 cgctgatctg gtgaacgacc gtacgtggga tacttccaag tctaactacg gttacggtat
      421 tgttgctatg aactctgatg gtcacctgac tatcaatggt aacggcgacg tagacaacgg
      481 tactgaactg gataacagct ctgttgataa cgttgttgct gcaaccggta actacaaagt
      541 tcgtatcgac aacgcaactg gcgctggcac tatcgctgat tacaaagata aagaacttat
      601 tcgggtaaac gacgctaaca ccaacgcgac cttctctgct gctaacaaag ctgacctggg
      661 tgcatacacc tatcaggctg aacagcgcgg taacaccgtt gttctgcaac agatggagct
      721 gaccgactac gctaacatgg cgctgagcat cccgtctgcg aacaccaata tctggaacct
      781 ggaacaagac accgttggta ctcgtctgac caactctcgt catggcctgg ctgataacgg
      841 cggcgcatgg gtaagctact tcggtggtaa cttcaacggc gacaacggca ccatcaacta
      901 tgatcaggat gttaacggca tcatggtcgg tgttgatacc aaaattgacg gtaacaacgc
      961 taagtggatc gtcggtgcgg ctgcaggctt cgctaaaggt gacatgaatg accgttctgg
     1021 tcaggtggat caagacagcc agactgccta catctactct tctgctcact tcgcgaacaa
     1081 cgtctttgtt gatggtagct tgagctactc tcacttcaac aacgacctgt ctgcaaccat
     1141 gagcaacggt acttacgttg acggtagcac caactctgac gcgtggggct tcggtttgaa
     1201 agccggttac gacttcaaac tgggtgatgc tggttacgag actccttacg gcagcgtttc
     1261 tggtctgttc cagtctggtg atgactacca gctgagcaac gacatgaaag ttgacggtca
     1321 gtcttacgac agcatgcgtt atgaactggg tgtagatgca ggttatacct tcacctacag
     1381 cgaagatcag gctctgactc cgtacttcaa actggcttac gtctacgacg actcaaacaa
     1441 cgataacgat gtgaacggtg attccatcga taacggtact gaagggtctg cggtacgtgt
     1501 tggtctgggt actcagttca gcttcaccaa gaacttcagc gcctataccg atgctaacta
     1561 cctcggtggt ggtgacgtag atcaagactg gtccgcgaac gtgggtgtta aatatacttg
     1621 gtaatattct tcactccgaa gaaattctgg taatttaatc taaataatgc ccgtcaagga
     1681 tttgacgggc attactgcaa aggacgcgca aatgttatct gtagttaaac ctcttcagga
     1741 atttggtaag ctcgataaat gtttgtccag atacggtacg cgcttcgagt ttaataatga
     1801 aaagcaagtt atattttcca gtgatgtcaa tagcgaagat actttcttta ttttagaggg
     1861 cgttatctct ctgcgtagag aagaaaacgt acttatcggt attacccagg cttcttatat
     1921 tatggggctg gctgatggtt taatgaaaaa cgatatacca tacaaattaa tatcagaagg
     1981 aaattgtacg ggatatcatc taccagccaa acaaaccatt acgcttattg aacaaaatca
     2041 actctggcga gacgcttttt actggttagc ctggcaaaat agaattctgg aattacgcga
     2101 cgtgcagctc attgggcata attcctacga acaaatccgc gcaacattat tatcaatgat
     2161 tgactggaat gaagaattgc gatcacgtat tggtgtgatg aattatatcc atcaacgtac
     2221 acgcatatcg cgttctgtcg tcgcagaagt tctcgctgct ttgcgtaaag gcggctatat
     2281 cgaaatgaat aaaggcaaac tggtcgctat caactgtttg ccttcagagt attaatcagg
     2341 atgcagggat aaccaacggt ttattcccgc ttagctcggt caccagatca ttgatgccat
     2401 cgctcatatt tttaaagcgc gtcaacggcg aacgagtgac caccacaaac gcgccaatat
     2461 tcttctgcgg gatcatcgcc atataggtaa tgaaaccgcc accaccgcct gtcttctgaa
     2521 taatccccgg acgaccctct ttcggggcca tatataccca gcctaaacca agcgcatccg
     2581 ctttgccggg gacatccatg ccaatcactt tggtaaactg cgcgcgctga taaatcaacg
     2641 tctgcatgcg atctgcctgg ttactgcgct gataaaaatc agatgataaa tactgctgca
     2701 tccagcgcat catatcgcca ggcgtggaat agacgccgcc gctgccaatt gccgccagcg
     2761 tgttattgca cggactggca ccgcgctcag caaccattaa acggcggcac tgatccggtg
     2821 agggggtgta ggtggtgtct ttcatcccca atggacgggt aatctgctct tcaaacaact
     2881 gggtataagg cttgccggac gcattcgcca gtgcatctgc cagcaggtcg aacgcaaggt
     2941 tagagtacgc cgcctgactg cctggcgcgg ctttcagctt cgccgtagaa aggtatttcc
     3001 agcgttgctc gcgcgttggc cagacaaaca ccggacgatg tgccgcgcca ccgggctgtt
     3061 cacggggcag ggcgctggta tgggttgcca gattcactag ggtaatgggc gtcccattgt
     3121 aggttggcac gcgtgcgcct ggcggagcat atttacttag cggatcgttt aacttcacgg
     3181 tcccctggtc gagcaatttc accagcattt cactggtcat tagcttggtg agggaagcaa
     3241 tacgcacgac cgaatccagc tgcgggcgaa cgttattacc aggtcgcgtt tcgccataac
     3301 tgcgaaagac gcgctggtta ccgtcgataa caaccagcgc catccccgtg gcaccgctgc
     3361 cgtaaaaaat atgatcggca taacgatcga caatatcaga ggcaaactcc ggttcagtga
     3421 tcggctgcgc cgcatggacg gaggtcaatg acgccgcaca cagcacggca gaaaaaagca
     3481 gactacgttt caacggtggt gtccatagat tgaatgaagg taactgagcg tatttatact
     3541 actcattgat ggattgcaaa gggcgaatta gtgcaataat agtgttaaaa acgtaacggc
     3601 gcgtaaacac gatctttttc ctgctcacca atgcctgcat ttgtgatcct gcgcgtgtta
     3661 atccgcttat gtaaacaatc tttggtttgt gaaatccgtc gaaacaattc ttatggtcag
     3721 gctggcgaac taagcgcctt gctatgggtc acaatgggcg gtttcatttg gctgacgctt
     3781 tgtagtggtc atgcggttaa tacacagcag ttgttaaaac gataagaagt tagcaggagt
     3841 gcatatgttt aagtcttttt tcccaaagcc gggagcgttt tttctctcgg cctttgtttg
     3901 ggcattgatt gccgttatct tctggcaagc cggtggggga gactgggtgg cgcgtatcac
     3961 cggcgcttcc gggcagatcc cgattagcgc cgcgcgtttc tggtcgttgg atttcctgat
     4021 tttttacgct tactacattg tttgcgtagg actttttgca tttttctggt ttatctacag
     4081 cccgcaccgt tggcaatact ggtcaatact cggtactgca ctgatcatct tcgtcacctg
     4141 gtttttggtg gaagtcggag tcgccgtcaa cgcctggtat gcaccgttct atgatctgat
     4201 tcaaaccgcg ctaagttcgc cgcataaagt cactatcgaa caattttacc gcgaagtggg
     4261 cgtctttctg gggattgcgc tgatcgcggt ggtgatcagt gtactgaaca acttctttgt
     4321 cagtcactat gtgttccgct ggcgtacggc gatgaacgaa tattacatgg cgaactggca
     4381 acaactgcgt catatcgaag gtgccgcaca gcgtgtgcag gaagacacca tgcgttttgc
     4441 ttcaacgctg gagaatatgg gcgtcagttt tatcaacgcc atcatgacgt tggtcgcctt
     4501 cctgccggtg ctggtaacgc tctccgcgca tgtgccggag ctgccgatta tcgggcacat
     4561 tccgtatggt ctggtgattg ccgctatcgt ctggtcgctg atggggaccg gattgctggc
     4621 agtggtaggg atcaaactgc cggggctgga gtttaaaaac cagcgagtag aggccgccta
     4681 ccgtaaagag ctggtttatg gtgaagacga tgccacgcgc gcgacgccgc ctacggtacg
     4741 cgagctgttt agcgccgtac ggaaaaacta ttttcgcctc tattttcact atatgtattt
     4801 caacatcgcc cgcattctct atttgcaggt cgataacgtt ttcggtttgt tcttgctgtt
     4861 tccgtcaatt gttgccggta cgattacgct cggcctgatg acgcagatta ccaacgtttt
     4921 tggtcaggtt cgcggtgctt tccagtacct gattaactca tggaccacac tggttgagtt
     4981 gatgtctatc tacaaacgtc tgcgcagctt tgaacatgag ctggatggtg acaaaattca
     5041 ggaagtaacc cataccttga gctaaaaagg agaagtaatg tcgcgcgtca atcatctatc
     5101 ttcgctgtca ttgttggcgg ttctggtttt agcggggtgt agtagtcagg ctccgcaacc
     5161 gttgaaaaag ggcgaaaaag cgatagatgt agcgagcgtc gtgcggcaga agatgcctgc
     5221 gagcgtgaaa gaccgcgatg cctgggcgaa agatctcgcc accacctttg aaagtcaggg
     5281 gctggcaccg acgctggaaa acgtctgctc ggtgctggcg gtggcgcagc aggagtcaaa
     5341 ttatcaggca gatccggccg ttccgggctt aagcaagatc gcctggcaag agattgaccg
     5401 tcgtgctgaa cggatgcaca ttcctgcttt tctggttcat accgcgctaa aaatcaaatc
     5461 gccaaacggc aaaagttata gcgaacggtt agattcggtg cgtacagaaa agcaattgag
     5521 cgcgattttt gacgatctga tcagcatggt accaatgggg cagacgctgt ttggttcgct
     5581 taatccggtg cgcaccggtg ggccgatgca ggtcagcatt gcttttgccg aacagcatac
     5641 caaagggtat ccgtggaaaa tggacggtac agttcgtcag gaagtcttca gtcgccgcgg
     5701 cgggttgtgg tttggtactt accatttact gaattatccc gccagctata gcgcaccgat
     5761 ataccgcttt gctgatttta acgctggctg gtacgccagc cgtaatgccg cgtttcagaa
     5821 cgcggtcagt aaggccagcg gcgtgaagct ggcactggac ggtgatttaa ttcgttacga
     5881 cagtaaagaa cccgggaaaa ccgaactggc aacgcgcaaa ctggcgggaa aactggggat
     5941 gagcgacagc gaaattcgcc gtcagttaga gaaaggtgac agcttctctt ttgaggagac
     6001 ggcgctgtac aagaaagttt atcaacttgc cgaagcgaaa acgggtaaat cactaccccg
     6061 cgaaatgttg cctggcattc aactggaaag cccgaaaatc acccgcaacc tgactacggc
     6121 ctggtttgcg aagcgcgtag acgaacggcg ggcgcgttgt atgaaacagt gatcaaacag
     6181 gaatgtcatg ccagataagg cgttttaggc cgtatctggc aacgtaaatc ttactggcgg
     6241 cgatgtcgcc agtgcagcca tagcgccacc actgcaaaaa tgaggcagcc tactaaaaac
     6301 gggatcagcc cgaaaatggt gccgacacct aaaccaattt ccacccgtgg gcgacctgtt
     6361 tcctggacct gcattaaacg ctcatagacg ccgggcgcat gcactaacag gttcagcaat
     6421 tgcgccccag cccaaaagca aaacagcaca aacaacgcat aggcaatgtt gccaggcgtt
     6481 gaagaggcat tgcgatattt tccgctaaac agcgattttg acagggtgaa atcagccata
     6541 aaaacctccg gaagtcatct gcttctcttc tggtgtgata ccagcggctt atggcaagtc
     6601 tgacaggtca tttagattgt caatatcaga atcatggtaa attgatgttg ggaatattcc
     6661 cgaagcgtga atcttcgttg tgagtcacaa aatggcaacc ttaagttaaa tttattaaca
     6721 attacgaaaa tgttgtctac accacagacg gttgttacag aatatgcaag gatgttgttt
     6781 ttcgttaacg gagctgccat gaatctgcct gtaaaaatcc gccgtgactg gcactactat
     6841 gcgttcgcca ttggccttat attcattctt aatggcgtgg tggggttact gggatttgaa
     6901 gcaaaaggtt ggcagaccta tgccgtcggt ctggtgacgt gggtgattag tttctggctg
     6961 gcggggttga ttattcgtcg tcgcgatgaa gaaactgaaa acgcccaata agaatattat
     7021 cgggcgttaa aatattacat tgtggttttc aatgcgttat ccgcagcgtg acgctccagc
     7081 gccagctcaa tcaaacgtgt gatcagatcg gtgtaaccca gaccgctggc ttgccacagc
     7141 ttcggataca tactgatgtt ggtgaagcca ggcagtgtgt tgatctcgtt gatcaccact
     7201 tcgttctctg gggttaaaaa cacgtctaca cgcgccatgc ctgcgcatcc caacgtttga
     7261 taagcctgaa cggcaatcgc ccggatctta tcgttgattt ctggcgcaat ggctgccgga
     7321 actaccactt tcgcgccatc ttcgtcaatg tacttggtgt cgtaggcata gaagtcgctg
     7381 gtgagtacga tctcgccaca ggtgctggct tgcgggttgt cgttgcccag aactgcgcat
     7441 tcgatctcac gacctttgat cccttgctca acgatcactt tatgatcgaa ttcgaacgcc
     7501 agatcgacgg caattgcgta ctgttcttca ctggttactt tgctgacacc aacagaagag
     7561 ccctgattag ccggttttac aaacagtggt aacctcagtt tagactccac ttcggcaaaa
     7621 ctaatgttgt gacgattggc gcgcgtcagg gtaataaatg gcgcaatgtt cagcccggca
     7681 tcgcgcagca gacgtttggt aacatcttta tccatacagg ctgctgaagc cagaacatca
     7741 gaacctacaa acggtaaatt ggcgacccgc agcattccct gcaaggaacc atcttcgccc
     7801 agcgtaccgt ggacaatcgg gaaaatgaca tccaccgtcg gtaacggctg accgttttgc
     7861 gcgtcgataa gctgatgctc atgtttactt ggcacctgcg caaggctagt cgccgaaggg
     7921 cgcaacgcaa tatgggcagg atcgtctgca tttagcagat aattgctggc atcgctgacg
     7981 tgccattgcc cttgtttatc aatgcccagc agcacaacgt cgaagcgact tttatcaatg
     8041 gcatcgacaa tgttttttgc cgattgcaga gacacttcat gttccgctga tttaccacca
     8101 aaaacgattc ctacccgcag tttttccatc ttaaaaacct atcccgtcta acacaaagtg
     8161 catacattac cacgacaaaa cgggggattc gcggccttct gacagaatgt tgcaatcttc
     8221 tgctgacaaa gcgtgcaacg taatggtgaa gaaagtgcgt tattacaaag atgtgcgcga
     8281 gatcacaaaa atgatgaacg ggaagctaat ttattcctgg cttaaatggc catgcggtga
     8341 gttttttctc ttagttgtaa gtaaacgcag agaatatatt tcataacttt tgtttataat
     8401 aaaggttgat aattaaaggc ctgttctttg tgtggttaat tatttaaata tgagaaacgt
     8461 ttcgctggta atcaaacaaa gaacatttgc gcaaagtatt tcctttgtca taaaaataat
     8521 acttccagac actatgaagt tgtgaaacat aatgttaact tctccatacg ttggataagg
     8581 aaatacagac atgaaaaatc tcattgctga gttgttatta aagcttgccc aaaaagaaga
     8641 agagtcgaaa gaactgtgcg cgcaggtaga agctttggag attatcgtca ctgcaatgct
     8701 tcgcaatatg gcacaaaatg accaacagcg cttgattgaa caggtggagg gggctcttta
     8761 cgaggtaaag cctgatgcca gcattcctga cgacgatacg gagctgctgc gcaattatgt
     8821 aaagaagtta ttgaagcatc ctcgtcagta aaaagttaat cttttcaaca gctgtcataa
     8881 agttgtcacg gccgagactt atagtcgctt tgtttttatt ttttaatgta tttgtacatg
     8941 gagaaaataa agtgaaacaa agcactattg cactggcact cttaccgtta ctgtttaccc
     9001 ctgtgacaaa agcccggaca ccagaaatgc ctgttctgga aaaccgggct gctcagggcg
     9061 atattactgc acccggcggt gctcgccgtt taacgggtga tcagactgcc gctctgcgtg
     9121 attctcttag cgataaacct gcaaaaaata ttattttgct gattggcgat gggatggggg
     9181 attcggaaat tacagccgca cgtaattatg ccgaaggtgc gggcggcttt tttaaaggta
     9241 tcgatgcctt accgcttacc gggcaataca ctcactatgc gctgaataaa aaaaccggca
     9301 aaccggacta cgtcaccgat tcggctgcat cagcaaccgc ctggtcaacc ggtgtcaaaa
     9361 cctataacgg cgcgctgggc gtcgatattc acgaaaaaga tcacccaacg attctggaaa
     9421 tggcaaaagc cgcaggtctg gcgaccggta acgtttctac cgcagagttg caggatgcca
     9481 cgcccgctgc gctggtggca catgtgactt cgcgcaaatg ctacggtccg agcgcgacca
     9541 gtgaaaaatg tccgggtaac gctctggaaa aaggcggaaa aggatcgatt accgaacagc
     9601 tgcttaatgc tcgtgccgac gttacgcttg gcggtggcgc aaaaaccttt gctgaaacgg
     9661 caaccgccgg tgaatggcag ggaaaaacgc tgcgtgaaca ggcacaggcg cgtggttatc
     9721 agttggtcag cgatgccgcc tcgctaaatg cggtgacgga agcgaatcag caaaaacctc
     9781 tgcttggact gtttgctgac ggcaatatgc cagtgcgctg gctaggaccg aaagcaacgt
     9841 accacggcaa tatcgataag cccgcagtca cctgtacgcc taatccgcaa cgtaatgaca
     9901 gtgtaccaac cctggcgcag atgaccgaca aagccattga attgttgagt aaaaatgaga
     9961 aaggattttt cctgcaagtt gaaggtgcgt caatcgataa acaggatcac gctgcgaatc
    10021 cttgtgggca aattggcgag acggtcgatc tcgatgaagc cgtacaacgg gcgctggaat
    10081 tcgctaaaaa ggatggcaac acgctggtca tagtcaccgc tgatcacgcc cacgccagcc
    10141 agattgtcgc gccggatacc aaagctccgg gcctcaccca ggcgctaaat accaaagatg
    10201 gcgcagtgat ggtgatgagc tacgggaact ccgaagagga ttcacaagaa cataccggca
    10261 gtcagttgcg tattgcggcg tatggcccgc atgccgccaa tgtcgttgga ctgaccgacc
    10321 agaccgatct cttctacacc atgaaagccg ccctggggct gaaataaaac cgcgcccggc
    10381 agtgaatttt cgctgccggg tggttttttt gctgttagca accagactta atggcaaatc
    10441 tcgggcgcat acgctcatgg ttaaaacatg aagagggatg gtgctatgaa aattacatta
    10501 ctggttacct tgcttttcgg tctggttttt ttaaccacag tcggcgctgc cgagagaacg
    10561 ttaaccccac aacaacagcg tatgacctcc tgtaatcagc aggcgacggc gcaggcgttg
    10621 aaaggggatg ctcgtaagac ctacatgagt gattgcctga agaacagcaa gtctgcgcct
    10681 ggtgaaaaaa gtttgacgcc acagcagcaa aagatgcgcg aatgtaataa tcaagcaacg
    10741 caacaatctc tgaaaggtga tgatcgtaat aagtttatga gtgcctgcct caagaaagcc
    10801 gcctgatacc tgatagtgct aacgggtgag ctacgaaagt ggctcacccg aaatttcata
    10861 cttctgcctt tagccccgtc tctataattt gggaaaattg tttctgaatg ttcccaaaaa
    10921 taatgaatga tgaaaacttt ttcaaaaaag cggcggcgca tggggaggaa cctcctttaa
    10981 ctcctcaaaa cgaacatcag cggtccgggc tgcgcttcgc ccgtcgcgtc agactacccc
    11041 gtgcggttgg cctggctggc atgttcttac cgattgcttc aacgctggtt tcgcacccgc
    11101 cgccgggctg gtggtggcta gtgttggtcg gctgggcgtt cgtctggccg catttagcct
    11161 ggcagatagc gagcagggcc gtcgatccgc ttagccggga aatttacaac ttaaaaaccg
    11221 atgcagtatt agcgggaatg tgggtaggcg taatgggcgt aaacgtgctg ccttccaccg
    11281 ctatgttgat gattatgtgt ctgaatttga tgggggcagg cggcccccgt ctgtttgtcg
    11341 cgggtctggt gttgatggtg gtttcctgcc ttgtcaccct cgagcttacg ggcattaccg
    11401 tgtcgttcaa tagtgcgccg ctggaatggt ggctctccct tcccattatt gtcgtttatc
    11461 ctctgctgtt tggctgggtc agctaccaga cggcaaccaa actggcggaa cataaacgca
    11521 ggttgcaggt catgagtacc cgcgacggca tgacgggcgt gtataaccga cgtcattggg
    11581 aaactatgtt acgcaatgaa tttgataact gtcggcggca taatcgcgat gcaacgttac
    11641 tgattatcga tatcgaccat ttcaagagca tcaacgatac ctggggtcat gatgtgggcg
    11701 atgaagcgat tgtggcgctt acccgacagt tacaaattac cctgcgcggt agcgatgtga
    11761 ttggtcggtt tggcggcgat gagtttgcag taatcatgtc cggtacgcca gctgagagcg
    11821 ccattaccgc catgttacgg gtgcgtgaag ggctaaacac gttacgcttg ccgaatacgc
    11881 cacaggtaac tttacggatt agtgtggggg ttgcgccgct gaacccacaa atgagtcact
    11941 atcgtgagtg gttgaaatcg gcagatttgg cgctttacaa agcaaagaaa gccggacgta
    12001 accgcaccga agtggcggcc tgacgtcagg cgaaagtcat caggatttgc tgagtttttc
    12061 tgatttttcc atacacttcg tcatcgcttc gatcactgcg gcacggaagc ctttctcttc
    12121 cagtacgcgt accgcttcaa tggtggtgcc tcccggtgag cagaccatat ctttcagtgc
    12181 ccccggatgt tctcccgttt ccagcaccat ttttgcggaa cccattaccg cctgagcggc
    12241 aaatttatac gcctgggcgc gtggcatccc gcccagcacg gcggcgtcgg ccatcgcttc
    12301 gataaacata aatacgtagg ctggcgaaga accgctcaca ccgactaccg ggtggatcat
    12361 cggctcagca attacttccg cttcgccaaa gcagcggaaa atattcagca catcagcggt
    12421 atcttctggg gttaccagcg cgtttggcgt tacggaggtc atcccggcat taaccagcgc
    12481 gggagtgttc ggcatggcgc ggataatttt ccggtcatgg cccagcgcgc gggcaagctg
    12541 gtcgagcgtg acacctgcag caatagaaac gaccagagag tctttattca ggctggaggt
    12601 gatttcgcta agcactttaa tcatgatgcc aggtttaacg gcagcaaaaa tgatgtcggc
    12661 gatttgcgcc acttcttgcg ccgattctgc ggcgttgatg ccgaactggt catgcagggc
    12721 ggcgacttta ttcggggagg gggtgtatac ccagatttgc cctggaagca cctgaccgct
    12781 ggcaatcaga ccgccgagaa tggcttttcc catattgccg cagccaataa aaccgatttt
    12841 cttttccatt gcctcactcc tgccgtgaaa ttcattgttt tgataatcgc tggcagaagc
    12901 ataaacagaa ctatgccgga aggcaaaagc gcgacacaat agaggattac ccaacaaagg
    12961 atgactttat gacaatttgg gtggatgctg acgcgtgtcc caatgtaatt aaagagattt
    13021 tgtatcgcgc ggcggaacgt atgcagatgc cgctggtact ggtagcaaac cagagtttac
    13081 gcgtgccgcc atcgcgattt attcgtacgc tgcgcgtcgc ggcaggtttc gacgttgccg
    13141 ataacgaaat tgtccggcag tgtgaagcgg gcgatttggt gatcaccgca gatatacctt
    13201 tggctgctga agccatcgag aaaggcgcag cggcgcttaa tccgcgcggc gaacgttaca
    13261 cgccagcgac cattcgtgag cgcctgacga tgcgcgattt tatggatacc ttacgtgcca
    13321 gtgggatcca gaccggcgga ccagatagcc tttcacaacg tgaccgccag gcctttgccg
    13381 cggagctgga gaagtggtgg ctggaagtgc aacgtagtcg tggctaaatg taatttatta
    13441 tttacacttc attcttgaat atttattggt atagtaaggg atgtattgag attttcactt
    13501 taagtggaat tttttcttta caatcgaaat tgtactagtt tgatggaatg atcgctattc
    13561 tcatgacacc ggctttcgcc gcattgcgac ctattgggga aaacccacga tgacacaacc
    13621 tctttttctg atcgggcctc ggggctgtgg taaaacaacg gtcggaatgg cccttgccga
    13681 ttcgcttaac cgtcggtttg tcgataccga tcagtggttg caatcacagc tcaatatgac
    13741 ggtcgcggag atcgtcgaaa gggaagagtg ggcgggattt cgcgccagag aaacggcggc
    13801 gctggaagcg gtaactgcgc catccacagt tatcgctaca ggcggcggca ttattctgac
    13861 ggaatttaat cgtcacttca tgcaaaataa cgggatcgtg gtttatttgt gtgcgccagt
    13921 atcagtcctg gttaaccgac tgcaagctgc accggaagaa gatttacggc caaccttaac
    13981 gggaaaaccg ctgagcgaag aagttcagga agtgctggaa gaacgcgatg cgctatatcg
    14041 cgaagttgcg catattatca tcgacgcaac aaacgaaccc agccaggtga tttctgaaat
    14101 tcgtagcgcc ctggcacaga cgatcaattg ttgattttag agcgcctata cttaacgttc
    14161 attccgtgaa ataaggaaga acgatgccaa cgaaaccgcc ttatcctcgt gaagcatata
    14221 tagtgacgat tgaaaaagga aagccaggac agacggtaac ctggtaccaa ctcagagccg
    14281 atcatcctaa accagactcg ttgatcagtg aacatccgac cgctcaggaa gcgatggatg
    14341 cgaaaaaacg ctatgaggac cctgacaaag agtgaccgca tcagactgct cggaagggat
    14401 tctgagtgcc actacaaggg atctgcgtca catttttcat aattcatgtt tttctaataa
    14461 ttagaatatt aaacaataac aatccattac tggaatcatt tggaatcttt acattatgcc
    14521 gtgcacgtct gctgctacgc tttttgtcat ttgtagcaca agtaagtgtc agcagtggtg
    14581 cttcacactt gcccggtaat taacgacgaa agaaaagtaa ggtggatgaa caatgagtgc
    14641 gtcgttggcg atcctgacca tcggcattgt acctatgcag gaggttttgc cgctcctgac
    14701 ggaatacatt gacgaagata atatttccca tcatagcctg ctggggaagt taagtcgtga
    14761 agaagtgatg gcggagtacg cgccagaagc aggcgaagac accattctca cattattaaa
    14821 tgacaaccag ctggcccatg tttcgcgtcg taaagtggag cgagacctgc aaggtgtggt
    14881 tgaagtgctc gataatcagg gttatgacgt cattttatta atgagtacag caaacattag
    14941 tagtatgact gcgcgtaata cgatctttct tgagccgtcg cgaatattgc ctccactggt
    15001 ttcctctatt gttgaagatc atcaggtggg ggttatcgtt ccggttgagg agatgctgcc
    15061 cgttcaggcg caaaaatggc aaattttgca gaaatcgccg gtattttcat tgggtaaccc
    15121 cattcatgat tcagaacaaa aaatcattga tgcagggaaa gaattactgg caaaaggggc
    15181 tgatgtcatc atgctggatt gtttaggatt tcaccaacgt catcgcgatt tactgcaaaa
    15241 acagctcgat gttcctgtct tgctgtctaa cgtattgatt gcacggctgg ctgcggaatt
    15301 actggtgtaa ttttgcgtga cagccagcgc ctctggcccc tatagtgaag tagatgttca
    15361 actaccagac agggccagtt tatgcttcaa agtaatgagt acttttccgg caaagtgaaa
    15421 tcaatcggct tttccagcag cagcactggt cgcgccagcg tgggtgttat ggttgaaggc
    15481 gaatacacct tcagcaccgc tgagccggaa gagatgacgg taatcagtgg cgcgctgaat
    15541 gtgttactgc ctgatgcgac cgactggcag gtgtatgaag ccggttcggt gtttaatgtt
    15601 cccggtcaca gtgagtttca tctgcaagtt gccgaaccca cctcttatct gtgccgctat
    15661 ctgtaattcc tcgccttccc cttgaacggg agggcatttt tctgaaatat cctctcttta
    15721 acccataata atatttcctt tgctgcgatt ttttcaattt ccgatatatt cataattcat
    15781 caaggttgat ataaatatca gtgaagatct ccagatattg ttgcggaact ggctacgata
    15841 aaagataaat cagatgatga atggtggcgt gcattgctgc aaagtcgcaa tgatcatttg
    15901 cgccaaacgg cattacgcaa cgcacatacc ccggcgtcgt tgttaacgac attgaccgag
    15961 tctcggcatc ggtcgctagc tatgaataat ccacagctcg ctgccgatgt gaaaacgacg
    16021 tggttaaaag aggatccatc attactctta tttgtcgaac aacccgatct ttcgctgtta
    16081 cgggatttag tgaaaaccgg ggcaatgcgg aaaattcgca gtgaagcgcg tcactggctt
    16141 gaggaaaaac aatgacacaa cgtccatgga gcaagttaca gcataaaacg cataatattg
    16201 ctgcgctaaa aattattgtc cgccgtagcg aataattata tccctggtgt ggcttcgtac
    16261 gccggataag acgcggcagg cgtcgcatcc ggcattaaag gaaaatcagc aattaacgtt
    16321 gtgcttcgcc acctaatcct tcaatcaggt tttgaattaa cgctgccagt tcaccagtca
    16381 tcaggatgaa atcggcatca aaacgctggg cgaaatcttc acggtcgata tcttcgtttt
    16441 gatcgcgcag ctcgtcgcaa aacttcagac gcttgagcga accatcgtcg cacatcacaa
    16501 actgaatgcg ctgctgccag tcgagcgcca gtttagtcac cacttttccg gcttcgatgt
    16561 gattggtgat ctcttcgctg gtcagatctt gtttcttcgc gcggatcacg ccgccatctt
    16621 ccagcaacga tttcagctcg gcttcatcaa gcagctggaa gccctgtgcc gcgctaccgg
    16681 agcgaaccca ttcggtcagc gtcagttcaa tcgggttttc catgctcaat ggtaccacag
    16741 gtaacgaccc caggctttta cgcagcagtg ccagcgtatc ttccgctttt ttggcactgg
    16801 cgcagtccac cataatcaaa ccgttaaccg tgtcgatcca catcattgtc tggctaaaac
    16861 ggctgaaagc acgcggcaac agagagtgca gcacttcgtc tttcagcgaa tctttttcgg
    16921 ttttcttcag cttacgcgcc tgttccgctt ccagtttggc gattttcgct tccagcgcct
    16981 gcttaatcac cggagacggg aggatttttt cttctttgcg cgcgcagata acaatttgac
    17041 cattggcaac gtgcgttaac gcatcgctgt gcgatcccat cggaggaacc cagcccatct
    17101 tcgccatgtc ctggctgccg catggggtaa atgccatcga ggctagctgt ttttccatct
    17161 cttctgcacg cagcgaaatc tcgcggctaa gacggtaaac cattaaattt ttgaaccaca
    17221 gcatgataat ttccacggcc ttgttgttaa atttagcggg catgataacg aattgtcggc
    17281 ggccttgcat tgccaatccg gttgtccgtc tctacgctat tgatattgaa aaaaataagg
    17341 agagtaccgt gcgtataggt atcgatttag gcggcaccaa aactgaagtg attgcactgg
    17401 gcgatgcagg ggagcagttg taccgccatc gtctgcccac gccgcgtgat gattaccggc
    17461 agactattga aacgatcgcc acgttggttg atatggcgga gcaggcgacg gggcagcgcg
    17521 gaacggtagg tatgggcatt cctggctcaa tttcgcctta caccggtgtg gtgaagaatg
    17581 ccaattcaac ctggctcaac ggtcagccat tcgataaaga cttaagcgcg aggttgcagc
    17641 gggaagtgcg gctggcaaat gacgctaact gtctggcggt ttcagaagca gtagatggcg
    17701 cggcagcggg agcgcagacg gtatttgccg tgattatcgg cacgggatgc ggcgcgggcg
    17761 tggcattcaa tgggcgggcg catatcagcg gcaatggcac ggcaggtgag tggggacaca
    17821 atccgctacc gtggatggac gaagacgaac tgcgttatcg cgaggaagtc ccttgttatt
    17881 gcggtaaaca aggttgtatt gaaaccttta tttcgggcac gggattcgcg atggattatc
    17941 gtcgtttgag cggacatgcg ctgaaaggca gtgaaattat ccgcctggtt gaagaaagcg
    18001 atccggtagc ggaactggca ttgcgtcgct acgagctgcg gctggcaaaa tcgctggcac
    18061 atgtcgtgaa tattctcgat ccggatgtga ttgtcctggg ggggggggat gagcaatgta
    18121 gaccgtttat atcaaacggt tccgcagttg attaaacaat ttgtcttcgg cggcgaatgt
    18181 gaaacgccgg tgcgtaaggc gaagcacggt gattccagcg gcgtacgcgg cgctgcgtgg
    18241 ttatggccac aagagtaaaa aacgtaggca attggcgcat catgcctgat gcgacgcttg
    18301 ccgcgtctta tcaggcctgc aaaatgtgcc agaaccgcgt agggcggata aggcgttcac
    18361 gccgcatccg gcaataagta atgaacaccg agactataac ctaccccagt ggtttcgcca
    18421 gcaccggagt atccgccgct tgctggcgct tatagcgacc atacagcagc aacgacgaca
    18481 tcgcagcaaa cgaaagcagg gcggcaggca gcgccacgta attatatgcc agccccagcg
    18541 tcagcatcat accaccgcaa tatgcgccga cggcgctacc gaggttaaac gctatttgcc
    18601 cacctgcggc acctaataac tctccgcctt tggcgttttg tagtaacaat atttgtagcg
    18661 gtgctgaaag ggcaaataat cccgcgcaac aaataaaagc aaaaataagc gacgttgttt
    18721 tcatgccgcc gcagaaaaag aacatcagca gtgccagtac aattataaag tcagtcactg
    18781 ctgcaatgcg cagtggtgaa taacgtcctg aaatcctgcc acttagcata tttcccagca
    18841 ccatccctag cccaactaac atcataataa aggtcatcgc cgtttccgaa aaaccggaaa
    18901 taaacatcat gtatggcttt acgtagctga accaggcaaa cacacctgcg ttgccaaaca
    18961 tggtggcggc gaaaattaac cacggggccg ggctgcgcaa aaagtgaaat tgttcgcgca
    19021 gctttccttt cgcctcgtcg cgaatatccg gcacccaaaa atagaccgat gccatcaccg
    19081 caatattaaa aacagcgatc aataaaaagg tgtaacgcca gctaaattcc tgacttaaat
    19141 acgttcccag cggaatgccc agcaaattgg cgactgtcat cccggaaacc atccccgcca
    19201 cggcggcggt gacttttccg ggtttgataa tttttgataa cacgatcgcg ccgacgccaa
    19261 aaaatgcgcc atgcggaaag ccggatacca gccgaccaat ggcgagcatc aggtaagacg
    19321 aagagagcgt gaacatggcg ttgccaatga cgcacaacgc caccagaaac aacaagatat
    19381 gtttgagtga gtagcggctg gaaaagagtg cgatgattgg cgcaccgacc accactccca
    19441 gtgcataata cgagatcata tgcccggcag caggaatcga aattcctacg ttatgcgcca
    19501 gctccgtgag cacgcccata atgccaaatt cggccatccc caaaccaaac gtgcccagag
    19561 ccaaagataa aatgactttt ttcataccac catcacaacc accagaacgg ctaccaatca
    19621 ccgtagccaa taaagtgata gcttgcagga taacgaccag caaggccaag agataattcg
    19681 cccctctgta ttcattatcc tgctgaatag ttatttcact gcaaacgtac tttccagttt
    19741 gctgtagccc aggccgttga tctttttcac tttgatctgc accggaatac gctctttcat
    19801 cgcttctacg tggctaatca caccgatggt tttgccactg gcgttcaggg catccagcgc
    19861 atcaagggcg gtatccagcg tttcgctatc cagcgtgcca aaaccttcat caaggaacag
    19921 cgagtcaata cgtgttttat ggctgaccag atccgaaagc gccagcgcca gcgccagact
    19981 aacgaggaaa ctttcgccgc cggaaagggt acgggtatcg cgtaccgcat ctgcctgcca
    20041 ggtatcaaca acctcgactt ccagcgcctc gctggctttg cgctgtaaca gatagcgccc
    20101 gtgcagccgg gtaagttgct gattagcgag atggactaaa ttatccagcg tcagcccctg
    20161 ggcaaacttg cggaatttat cgccctcttt ggaacctatt agcgaattca gatatcccca
    20221 gtcctcaacc tgctgcgtca tttgagcaat ttgctgcatt aaggtttgtt gttgctgacg
    20281 gttatctgca tcctgcttca gctgctggcg aatctcgcct tgactcgtgg tgttttcacg
    20341 caacttttgg tgagtttgcg ctaactcttg ctgaatctgc tccaccgtca cagtgagagc
    20401 caacccgtca ggtcggtgtt gttgatgctg tgccagcgtt tctgctgtct gagtgaccag
    20461 cgtttgcgcc tgacggcgct ggttttccag attctgcttg agctgttcca gctgcgttag
    20521 tgtttgttca tccattagcg ccgcaaggaa cgcctgctga tcgtcaaaga cgctggcctg
    20581 tagcgcggtg tcaaactgcg cctgggcttt ttgcagactt tgcgccgcca gaacatcctg
    20641 ttgctgtaac gtctgctgct ggctgtgtaa tgcgagacat tgttcatgaa cctgccgcca
    20701 gttatccagc gccacagttt cttcgctgtg cgggagatca tcactttgcg gcaacgtttc
    20761 cagaatcggc gtcagctgct gaatacggtt ttgcagcgtg gttaattcgt tctggcgttg
    20821 ctgccagctc tgcgcttctt gctgacgtgt cgccaaccag ctctcttctt catcttcctg
    20881 tggcaatgtc agtgcataac ccgccaatgc cgttaagagc tgctgctggc gttgttcaat
    20941 ttgctgttga tactggataa tttgctgatt atgcgcggca atctgccctt gtaattcatg
    21001 tcgttggctg agtaaccgca gctggcgttt gtgctcatct tgtgcatcca gccacggttg
    21061 aatatcgtcc tgtggctgca aggtgatatt gaggctggcc gtgacggctt gccattgttg
    21121 agtaagtgct tgctcatctt gtcggaggct ttgcgcttcg ttttcatcgc gctgaagctg
    21181 cttcgttaat gcatccagtt gcccgcgcag cgccgcacct tcttcgccga gctttttaac
    21241 ttcgttttcc agcgccagta atcgagactg attaacacca ggctccagcg cctgatacgc
    21301 cttgaccgcc gggtggctgg tggaaccaca aagtggacaa ggctgacccg cctgtaactg
    21361 tgcacgctgg gcttccagcg ttttgatgcg cgcttcctgc tcgcaaatgg ttttcacatc
    21421 ggcaagttgc tgcgtctttt ctttataacg ctggcgcatt tcgttaagtg cgacattacg
    21481 ttgcgtctgt tccagagatc cattctggat agtgaccatt aactgcgcca gacgtttttg
    21541 ttggggaaca atctgcccat gcagcgcgac caggcgctga cgcagtgggc gttgctcagc
    21601 atgttgcgcc agggcggtag caacttcatc ggcggttaac gtcaacgtga tcgccgcaag
    21661 cgcattaagt ttttgctcag catgggttaa ctgttgctgc cattgccgca gatgctcgcg
    21721 atcgctggtt tgttgggaga actgcgcacg ccaacccgcc agttcgttgt tccactgacg
    21781 gaagcggtcg tgttcctgta accaggtatt caggctttgt tgctgctgct gtaattctgc
    21841 tgactgcttc gccgcgtggt ggcgaatgct cgcgcgaagc gccattgtgc tctgtaagcg
    21901 agtatttact tcttcaatct gctggcgagt atgcgccagc gccgcgctgt gttctgcgat
    21961 gcgttcccag tgcggacgaa gatttcgtgc cggttgtgcc agactgagcg ccgccagttg
    22021 aggttgcgct ttttcttctt cggctaacgc ctgttgcaag gcctgctgac ggcggctggc
    22081 ttcttgctgc aattcgtcca gacgcgttaa ccagtttagt gattgttgtt cttgctgctg
    22141 cgcggtaagt aactgttttt cttcgtcagt aagtacctgc aaactcgctg tcagcgattg
    22201 cacttgttcc ggcgtgagca acgcgacgcc gctggcctgc gcttgcagct tctccagctc
    22261 tgtgcgggcc gatttgtgct gctcaaaaac catcgccgag atttgcccgt agatttcagt
    22321 gccggttaac tcctcgagca attccgcgcg ttctttgggt ttggcattca ggaaggcagc
    22381 aaattgcccc tgcgaaagca gcatcgaacg ggtgaagcgc ccgtaatcca gcccggttaa
    22441 cgtcgctgtc agttccagct tatctttcac tttgtcggcg agaattttgc cgtcggcgca
    22501 gcgcgccagc tctacgcgtg gcacctgcaa attaccgtcg ggttggttac gcgcccgatt
    22561 ctggctccag aatgcacggt acgcttcacc tttcacttca aactccacct ccgccagaca
    22621 ttcggcggta tcgcgggtca tgagatcatt ttgcgattgt gaaacgttag agagacgcgg
    22681 agtttcgtga tacagcgcca gacaaatggc gtccagcagg gtggttttcc ccgcaccggt
    22741 tgggccggta atggcaaaca gcccgttgct ggcgaacggc tcgcgggtga aatcaatctt
    22801 ccattcgcct tttaatgagt tcaggttttt caggcgcagg ctgagaattt tcatgcttcg
    22861 tgttctccgg cgagggtatg caacgtcgtg gtgaaaagat gctgcagacg ttgctgctgc
    22921 gattcatcca gttcttccag tgccagacgg cgattgaaca cctcttcgac gctgagttcg
    22981 ctgagggttt cacgctgttg gctggctaac acacgctcgc gctgttcacg actccgacgt
    23041 accagcaata cttcgacagg caatgattcg gctaatgcct ggattttgcg ctgaatatca
    23101 tgcagatact catcagtagt gatttcgata tccagccaga caggtggctc ctgcgataca
    23161 tcgcgccact gttccagctg tgcggtaatc gacgccagat cgcctttcag caccgccatg
    23221 ggttgagtta ccggtacgtt caggttttcc acgctctcta atttgccgtt tgaaaatgtc
    23281 accagatgga catatttact cttaccgcat tcatcaaaac tcagtggaat gggggaaccg
    23341 caatagcgaa catgttccat gccgccaata atctgtgcgc ggtgaatatg cccgagcgcg
    23401 atgtagtcgg ctggtggaaa gttttgtgcc ggaaacgcgt ccagcgtgcc aatataaatg
    23461 tcacgcacgg cgtcactttt actggcaccc acggtcgtta aatgtcccgt ggcgatgatg
    23521 ggcagaggct gatcgccgcg cagtttgcag gcatcggcat agtgttgttg gtaataatcg
    23581 gtaatcgctg ccagtaaatg ctgctgtttt tcaataccgt taagccccgc ctggctggaa
    23641 ataatgtcac gcgggcgtaa aaacggaatg gggcacagca ctgcgcctgg cgtcccgtcg
    23701 cgacgaggca agatttgcgg cgcatgtccg gcgctggcga ccacggttgt attgaggaac
    23761 gccatgatat cgcgcgattc attcagcgtg gcgaccgagt catggtttcc tgccagtacc
    23821 accagatgac agccagtttg ctgtaaattg acaacaaaac ggttgtataa cgtgcgggcg
    23881 taactgggcg gcgagccggt atcgaaaaca tcaccagcaa caataatcgc atccacctga
    23941 tgggtttgtg ctgtctccag cagccagtca agaaaagcct gatgttcagc ttcgcggctt
    24001 ttactgtaga agttctggcc gagatgccag tctgaggtgt gaaggatgcg cataacggtt
    24061 ccctggcgaa aaagcatggt cgcgattata cccaaacaga tgtgccattt gcttttttct
    24121 gcgccacgga aatcaataac ctgaagatat gtgcgacgag cttttcataa atctgtcata
    24181 aatctgacgc ataatgacgt cgcattaatg atcgcaacct atttattaca acagggcaaa
    24241 tcatggcgag acgtattctg gtcgtagaag atgaagctcc aattcgcgaa atggtctgct
    24301 tcgtgctcga acaaaatggc tttcagccgg tcgaagcgga agattatgac agtgctgtga
    24361 atcaactgaa tgaaccctgg ccggatttaa ttctccttga ctggatgtta cctggcggct
    24421 ccggtatcca gttcatcaaa cacctcaagc gcgagtcgat gacccgggat attccagtgg
    24481 tgatgttgac cgccagaggg gaagaagaag atcgcgtgcg cggccttgaa accggcgcgg
    24541 atgactatat caccaagccg ttttcgccga aggagctggt ggcgcgaatc aaagcggtaa
    24601 tgcgccgtat ttcgccaatg gcggtggaag aggtgattga gatgcaggga ttaagtctcg
    24661 acccgacatc tcaccgagtg atggcgggcg aagagccgct ggagatgggg ccgacagaat
    24721 ttaaactgct gcactttttt atgacgcatc ctgagcgcgt gtacagccgc gagcagctgt
    24781 taaaccacgt ctggggaact aacgtgtatg tggaagaccg cacggtcgat gtccacattc
    24841 gtcgcctgcg taaagcactg gagcccggcg ggcatgaccg catggtgcag accgtgcgcg
    24901 gtacaggata tcgtttttca acccgctttt aacgccttgc tcatcggacg cagagcaggg
    24961 cttatgattt cttaactgga gtatcttacg tgctggaacg gctgtcgtgg aaaaggctgg
    25021 tgctggagct gctactttgt tgcctcccgg ctttcatcct gggtgcattt tttggttacc
    25081 tgccctggtt tttgctggca tcggtaacag gactgcttat ctggcatttc tggaatttat
    25141 tgcgcctttc atggtggttg tgggtggatc gcagtatgac cccgccaccg gggcgtggta
    25201 gctgggaacc gctactatac ggcttacacc agatgcagct gcgaaataaa aaacgccgcc
    25261 gtgaactggg caatctgatt aaacgctttc gtagcggcgc ggagtcgctg cccgacgcgg
    25321 tggtgctgac cacggaagag ggcggtattt tctggtgtaa cggtctggcg caacaaattc
    25381 ttggtttgcg ctggccggaa gataacgggc agaacatcct taacctactg cgttacccgg
    25441 agtttacgca atatctgaaa acgcgtgatt tttctcgccc gctcaatctg gtgctcaaca
    25501 ccgggcggca tctggaaatt cgcgtcatgc cttataccca caaacagttg ctgatggtgg
    25561 cgcgtgatgt cacgcaaatg catcaactgg aaggggcgcg gcgtaacttt tttgccaacg
    25621 tgagccatga gttacgtacg ccattgaccg tgttacaggg ttacctggag atgatggatg
    25681 agcagccgct ggaaggcgcg gtacgcgaaa aagcgttgca caccatgcgc gagcagaccc
    25741 agcggatgga agggctggtg aagcagttgc taacgctgtc gaaaattgaa gccgcgccga
    25801 cgcaattgct caatgaaaag gttgatgtgc cgatgatgct gcgcgttgtt gagcgcgagg
    25861 ctcagactct gagtcagaaa aaacagacat ttacctttga gatagataac ggcctcaaag
    25921 tgtctggcaa cgaagatcag ctacgcagtg cgatttcgaa cctggtctat aacgccgtga
    25981 atcatactcc agaaggcacg catatcaccg tacgctggca gcgagtgccg cacggtgccg
    26041 aatttagcgt tgaagataac ggaccgggca ttgcaccgga gcatattccg cgcctgaccg
    26101 agcgttttta tcgcgttgat aaagcgcgtt cccggcaaac cggcggtagc ggattagggt
    26161 tagcgatcgt gaaacatgct gtgaatcatc acgaaagtcg cctgaatatt gagagtacag
    26221 taggaaaagg aacacgtttc agttttgtta tcccggaacg tttaattgcc aaaaacagcg
    26281 attaatccgc ctttgtcatc ttttattgcc ataagccagt cgatgctggc ttattttctt
    26341 tgcagtcaaa atacgggcgt tagatttcac aacggttggt gattttttgt tcgcatgatt
    26401 agccatgtct ttttcacgga aatagtgttt tatactggtt ggtgatttct tatcgctata
    26461 tacctctggt ttttagatcc ctccttgctt taaaacgtta taagcgttta aattgcgctt
    26521 caggtgctgt catactgact gcattaacgc ggtaaatcga aaaactattc ttcgccgcgc
    26581 caggttggga gtatttcccg ctaaaattgt ttaaatatac cgctgtatca tccccaggga
    26641 ttggcacaaa aatttaacgt tacaacacca catccacagg cagtatgatt tatgacccat
    26701 caattaagat cgcgcgatat catcgctctg ggctttatga catttgcgtt gttcgtcggc
    26761 gcaggtaaca ttattttccc tccaatggtc ggcttgcagg caggcgaaca cgtctggact
    26821 gcggcattcg gcttcctcat tactgccgtt ggcctgccgg tattaacggt agtggcgctg
    26881 gcaaaagttg gcggcggtgt tgacagcctc agcacgccaa tcggtaaagt cgctggcgtg
    26941 ctgctggcaa cggtttgtta cttggcggtg gggccgcttt tcgctacgcc gcgtacagct
    27001 accgtttcct ttgaagtggg gattgcgccg ctgacgggtg attccgcgct gccgctgttt
    27061 atctacagcc tggtctattt cgctatcgtt attctggttt cgctctatcc gggcaagctg
    27121 ctggataccg tgggcaactt ccttgcgccg ctgaaaatta tcgcgctggt catcctgtct
    27181 gttgccgcta ttgtctggcc ggcgggttct atcagcacgg cgactgaggc ttatcaaaac
    27241 gctgcgtttt ctaacggctt cgttaacggc tatctgacca tggatacgct gggcgcaatg
    27301 gtgtttggta tcgttattgt taacgcggcg cgttctcgtg gcgttaccga agcgcgtctg
    27361 ctgacccgtt ataccgtctg ggctggcctg atggcgggtg ttggtctgac tctgctgtac
    27421 ctggcgctgt tccgtctggg ttcagacagc gcgtcgctgg tcgatcagtc tgcaaacggc
    27481 gctgctattc tgcatgctta cgttcagcac acatttggcg gcggcggtag cttcctgctg
    27541 gcggcgttaa tcttcatcgc ctgcctggta acggcagttg gcctgacctg tgcttgtgca
    27601 gaattctttg cccagtacgt accgctctct tatcgtacgc tggtgtttat cctcggcggc
    27661 ttctcgatgg tggtgtctaa cctcggctta agccagctga tccagatctc cgtaccggtg
    27721 ctgaccgcta tttatccgcc gtgtatcgca ctggttgtat taagttttac acgctcatgg
    27781 tggcataatt cgtcccgcgt gattgccccg ccgatgttta tcagcctgct ttttggtatt
    27841 ctcgacggga tcaaagcatc tgcattcagc gatatcttac cgtcctgggc gcagcgttta
    27901 ccgctggccg aacaaggcct ggcgtggtta atgccaacag tggtgatggt ggttctggcc
    27961 attatctggg atcgcgcggc aggtcgtcag gtgacctcca gcgctcacta aatcactgaa
    28021 catttgtttt aaccacgggg ctgcgatgcc ccgtggtttt ttattgtgtt gatgggttag
    28081 gaattgatgg aaagtaagaa caagctaaag cgtgggctaa gtacccgcca catacgcttt
    28141 atggcactgg gttcagcaat tggcaccggg ctgttttacg gttcggcaga cgccatcaaa
    28201 atggccggtc cgagcgtgtt gttggcctat attatcggtg gtatcgcggc gtatatcatt
    28261 atgcgtgcgc tgggggaaat gtcggtacat aacccggccg ccagctcttt ctcgcgttat
    28321 gcgcaggaaa acctcggccc gctggcaggt tacattaccg gctggaccta ctgctttgaa
    28381 atcctaattg tcgccatcgc cgatgtgacc gcttttggta tctatatggg tgtctggttc
    28441 ccgacggtgc cgcactggat ttgggtactg agcgtggtgc tgatcatttg cgccgtaaac
    28501 ctgatgagcg tgaaggtatt cggtgagctg gaattctggt tctcgttctt taaagtcgcc
    28561 accatcatca tcatgattgt cgccggtttc ggcatcatca tctgggggat tggcaacggc
    28621 gggcaaccga ccggtattca taacctgtgg agcaacggcg gcttcttcag taacggctgg
    28681 cttggcatgg taatgtcgtt gcaaatggtg atgtttgctt acggtgggat cgaaattatc
    28741 gggattaccg ccggtgaagc gaaagatcct gagaaatcga ttccgcgtgc gattaactcc
    28801 gtgccgatgc gtattctggt gttctacgtc ggtacgctgt tcgtcattat gtctatctac
    28861 ccgtggaatc aggttggcac tgccggtagc ccgttcgtgc tgacgttcca gcatatgggc
    28921 attacctttg ccgccagcat tcttaacttt gttgtgctga ccgcttcgct gtcggcaatt
    28981 aacagtgacg tatttggcgt aggccgtatg ctccacggta tggcagagca gggcagcgca
    29041 ccgaaaattt tcagcaaaac gtcgcgtcgc ggtattccgt gggttacggt gctggtgatg
    29101 actaccgcgc tgctgtttgc ggtgtatctg aactacatca tgccggaaaa cgtcttcctg
    29161 gtgatcgctt cgctggcaac cttcgccacg gtatgggtgt ggattatgat cctgctgtcg
    29221 caaatcgcct tccgtcgccg tttgccgcca gaagaagtta aggcgctgaa atttaaagtg
    29281 ccgggtgggg tagcaacgac catcggcggg ctgattttcc tgctctttat tatcgggttg
    29341 attggttatc acccggatac gcgtatctcg ctgtatgtcg gtttcgcgtg gattgttgtg
    29401 ctgttgattg gctggatgtt taagcgtcgc cacgatcgtc agctggctga aaaccagtaa
    29461 tccttgcgtt ctgtaagccg gttcaggagg aaatcctgat ccggctattc cgaaagttat
    29521 ccgcccccgt cctcctcccc caaatatcct tcagatgatg agtgatcctg cattaggcta
    29581 tggcaaggtg atcagatttt catcacaggg gaattatgat gttaaatgca tggcacctgc
    29641 cggtgccccc atttgttaaa caaagcaaag atcaactgct cattacactg tggctgacgg
    29701 gcgaagaccc accgcagcgc attatgctgc gtacagaaca cgataacgaa gaaatgtcag
    29761 taccaatgca taagcagcgc agtcagccgc agccaggcgt caccgcaggg cttcagccga
    29821 atgccgccgg cacgactgga gcagtttgcc gtcgatgtac cggatatcgg cccacaatgg
    29881 gctgcggatc agatttttta tcagatcttc cctgatcgtt ttgcgcgtag tcttcctcgt
    29941 gaagctgaac aggatcatgt ctattaccat catgcagccg gacaagagat catcttgcgt
    30001 gactgggatg aaccggtcac ggcgcaggcg ggcggatcaa cgttctatgg cggcgatctg
    30061 gacgggataa gcgaaaaact gccgtatctg aaaaagcttg gcgtgacagc gctgtatctc
    30121 aatccggtgt ttaaagctcc cagcgtacat caatacgata ccgaggatta tcgccatgtc
    30181 gatccgcagt ttggcggtga tggggcgttg ctgcgtttgc gacacaatac gcagcagctg
    30241 ggaatgcggc tggtgctgga cggcgtgttt aaccacagtg gcgattccca tgcctggttt
    30301 gacaggcaca atcgtggcac ggggggagct tgttacaacc ctgaatcgcc ctggcgcgac
    30361 tggtactcgt ttagtgatga tggcacggcg ctcgactggc ttggctatgc cagcttgccg
    30421 aagctggatt atcagtcgga aagtctggtg aatgaaattt atcgcgggga agacagtatt
    30481 gtccgccatt ggctgacagc gccgtggagt atggacggct ggcggctgga tgtggtgcat
    30541 atgctggggg aggcgggtgg ggcgcgcaat aatatgcagc acgttgctgg gatcaccgaa
    30601 gcggcgaaag aaacccagcc ggaagcgtat attgtcggcg aacattttgg cgatgcacgg
    30661 caatggttac aggccgatgt ggaagatgcc gccatgaact atcgtggctt cacattcccg
    30721 ttgtggggat ttcttgccaa taccgatatc tcttacgatc cgcagcaaat tgatgcccaa
    30781 acctgtatgg cctggatgga taattaccgc gcagggcttt ctcatcaaca acaattacgt
    30841 atgtttaatc agctcgacag ccacgatact gcgcgattta aaacgctgct cggtcgggat
    30901 attgcgcgcc tgccgctggc ggtggtctgg ctgttcacct ggcctggtgt accgtgcatt
    30961 tattacggtg atgaagtggg actggatggt aaaaacgatc cgttttgccg taaaccgttc
    31021 ccctggcagg tggaaaagca ggatacggcg ttattcgcgc tgtaccggcg aatgattgtg
    31081 ctgcgtaaga aaagtcaggc gctgcgtcgt ggcggctgtc aggtgctgta tgcggaagat
    31141 aacgtagtgg tatttgtccg cgtgctgaat cagcaacgtg tactggtggc aatcaaccgt
    31201 ggcgaggcct gtgaagtggt gctacccgcg tcaccgtttc tcaatgccgt gcaatggcaa
    31261 tgcaaagaag ggcatgggca actgactgac gggattctgg ctttgcctgc catttcggct
    31321 acggtatgga tgaactaacg ttttataacg ccttgtgtga cgcctgcgcc atcatccgcg
    31381 gataaaactg ccagaagcgg gttccgaggg cggtataatg ggcgtttaaa tcgtaccagg
    31441 agtcacgcag ggcatccaga cgtgggcgac ggcttgccat gccgtttaac acgttctgga
    31501 tgaaatccat atcgcgatag cgcaccagcc actgctctga ccacaagtaa ttgtttagat
    31561 tgataaaacg tggcggtgag tccggcaaaa tcgtcatcac ttgcttgcgg gcataacaga
    31621 taaattcctg tagcggaaag tccggcgaca gctgcgacca gtggcgggaa agaaagtgat
    31681 cccacatgac atccagcgta ataggcgcaa cgcggcgcgt ttcacgacga aaccactccc
    31741 gtgcttcgcg gacttccggc agattgtcag tcaatacgtc gatacgtcga tgcatatgaa
    31801 tgccagccac gacgtcgggc ggaaaacttt cttcaggatt tccgcgtacg aaatcagcca
    31861 gtaaattgcc ggaaagcgag ctttccgcga gatgggctaa atgcaggtga gctaaaaaat
    31921 tcatcgatta tattctatcc aaaggggggt aaaggttgca gggagagcgc cccggcacta
    31981 gactacccgc ctcttatttt agtctgagtc agtgtcatgc gcgttaccga tttcttcttt
    32041 gaattgcccg aatccctgat tgcccactac cccatgcctg aacgcagtag ctgtcgttta
    32101 ctgtcgctgg acgggcctac gggcgcgctg acgcacggta ctttcaccga tttactcgat
    32161 aagctcaacc ccggcgatct tctggttttt aataatactc gcgtgatccc ggcgcgcctg
    32221 tttgggcgta aagccagcgg cggcaagatt gaagtgctgg ttgagcggat gctcgacgac
    32281 aaacgcattc ttgcgcatat tcgcgcctcg aaagcgccaa aacctggcgc agaactgctg
    32341 ctgggcgatg acgaaagtat taacgcaaca atgaccgcgc gccacggcgc actgtttgaa
    32401 gtcgaattca atgatgatcg ttcggtactg gatatcctca acagtatcgg gcatatgccg
    32461 ctgccgccgt atatcgaccg tccggacgaa gacgctgacc gcgaacttta tcaaaccgtt
    32521 tatagcgaaa aaccgggcgc ggttgcagcc ccgaccgctg gtctgcattt tgacgagcct
    32581 ttgctggaaa aattgcgcgc caaaggcgtg gagatggcgt ttgtgacgtt gcacgttggt
    32641 gcgggcacct tccagccggt gcgcgtcgac accattgaag atcacatcat gcactcggaa
    32701 tacgctgaag taccgcagga tgtggtagac gcggtactgg cggcgaaagc gcgcggtaac
    32761 cgggtgattg cggttggcac cacttcagta cgttcgctgg aaagcgcggc tcaggcagcg
    32821 aaaaacgatc tcattgaacc gttcttcgac gatacccaaa tctttatcta tccgggcttc
    32881 cagtacaaag tggtcgatgc gctggtgacg aacttccact tgccagagtc gacgctgatt
    32941 atgctggttt cggcctttgc cggttatcaa cacaccatga acgcctataa agcagcggta
    33001 gaagagaaat atcgcttttt tagttacggt gatgcgatgt ttattacgta caatccgcag
    33061 gcaattaatg agcgcgtcgg ggagtaattc cgcggcgctg gtttaaaacg ttggactgtt
    33121 tttctgacgt agtggagaaa aaatgaaatt tgaactggac accaccgacg gtcgcgcacg
    33181 ccgtggccgc ctggtctttg atcgtggcgt agtggaaacg ccttgtttta tgcctgttgg
    33241 cacctacggc accgtaaaag ggatgacgcc ggaagaagtt gaagccactg gcgcgcaaat
    33301 tatcctcggc aacaccttcc acctgtggct gcgcccgggc caggaaatca tgaaactgca
    33361 cggcgatctg cacgatttta tgcagtggaa ggggccgatc ctcaccgact ccgggggctt
    33421 ccaggtcttc agccttggcg atattcgtaa aatcaccgaa cagggcgtgc acttccgtaa
    33481 cccgatcaac ggcgatccga ttttcctcga tcctgaaaaa tcaatggaga ttcagtacga
    33541 tcttggttcg gatatcgtca tgatctttga tgagtgtacg ccgtatcctg ctgactggga
    33601 ttacgcaaaa cgctccatgg agatgtctct gcgttgggcg aagcgtagcc gtgagcgttt
    33661 tgacagtctc ggaaacaaaa atgcgctgtt tggtatcatt cagggcagcg tttacgaaga
    33721 tttacgtgat atttctgtta aaggtctggt agatatcggt tttgatggct acgctgtcgg
    33781 cggtctggct gtgggtgagc cgaaagcaga tatgcaccgc attctggagc atgtatgccc
    33841 gcaaattccg gcagacaaac cgcgttacct gatgggcgtt ggtaaaccag aagacctagt
    33901 tgaaggcgta cgtcgtggta tcgatatgtt tgactgcgta atgccaaccc gcaacgcccg
    33961 aaatggtcat ttgttcgtga ccgatggcgt ggtgaaaatc cgcaatgcga agtataagag
    34021 tgatactggc ccactcgatc ctgagtgtga ttgctacacc tgtcgcaatt attcacgcgc
    34081 ttacttgcat catcttgacc gttgcaacga aatattaggc gcgcgactca acaccattca
    34141 taaccttcgt tactaccagc gtttgatggc gggtttacgc aaggctattg aagagggtaa
    34201 attagagagc ttcgtaactg atttttacca gcgtcagggg cgagaagtac cacctttgaa
    34261 cgttgattaa tattaataat gagggaaatt taatgagctt ttttatttct gatgcggtag
    34321 cggcaacggg tgcaccggcg caaggtagcc cgatgtcttt gattttgatg ctggtggtat
    34381 tcggtctgat tttctatttc atgatcctgc gtccacagca gaagcgcacc aaagaacaca
    34441 aaaagctgat ggactccatt gccaaaggtg atgaagttct gacgaacggt ggcctggttg
    34501 gtcgcgtaac caaagtagcg gaaaacggct acattgctat cgcgctgaat gacaccactg
    34561 aagtagttat taaacgtgac ttcgtagctg ccgtcctgcc gaaaggcacc atgaaggcgc
    34621 tgtaattaaa atttttccct aagggaattg ccgtgttaaa ccgttatcct ttgtggaagt
    34681 acgtcatgct gatcgtggtg attgtcatcg gtctgctgta tgcgcttccc aacctgtttg
    34741 gtgaggatcc ggctgttcag atcactggtg cgcgcggagt cgccgccagt gagcaaacgc
    34801 tgatccaggt ccagaaaacg ttacaagaag aaaaaataac tgctaagtct gtggcactgg
    34861 aagagggcgc tattcttgcg cgcttcgact ccactgacac ccagctgcgc gctcgtgaag
    34921 cattaatggg cgttatgggt gacaaatacg tcgtggcgct taaccttgcc ccggcaacgc
    34981 cgcgctggct ggcagctatt cacgctgagc cgatgaagct cggccttgac ctgcgtggcg
    35041 gcgttcactt cctgatggaa gtggatatgg acaccgcgct tggcaaactc caggaacaaa
    35101 atatcgatag cctgcgcagc gatctgcgtg aaaagggcat cccgtatacc accgttcgta
    35161 aagaaaacaa ctacggcctg agcattactt tccgcgatgc taaagctcgt gatgaagcca
    35221 ttgcgtatct gagcaagcgc catccggacc tggtgattag tagccagggc agcaaccagc
    35281 tgcgtgcggt aatgagcgat gctcgtctga gtgaagcgcg tgaatatgcg gtgcagcaga
    35341 acatcaatat cctgcgtaac cgtgtaaacc aacttggcgt agcggagccg gtggttcagc
    35401 gtcagggtgc tgaccgtatc gttgttgaac tgccaggtat tcaggacact gcgcgtgcga
    35461 aagagattct gggtgcgacg gcaacgctgg aattccgtct ggtaaacacc aacgttgacc
    35521 aggccgctgc ggcatccggt cgcgtaccgg gcgactctga agtgaaacag acccgcgaag
    35581 gtcagccagt tgtgctgtac aaacgcgtaa ttctgaccgg tgaccatatc accgactcca
    35641 cttccagcca ggacgaatac aaccaaccgc aggttaacat ctcgctcgat agcgctggtg
    35701 gtaacatcat gtctaacttc actaaggaca acatcggcaa accgatggca accctgtttg
    35761 tggagtacaa agacagcggt aagaaagatg caaatggtcg tgcggttctg gtgaaacagg
    35821 aagaggtgat taacatcgcc aacatccagt ctcgtctggg taacagcttc cgtatcaccg
    35881 gcatcaacaa cccgaacgaa gcccgtcagc tgtcactgct gctgcgtgcc ggtgcgttga
    35941 tcgcgccgat tcagattgtt gaagaacgta ccattggccc aaccctgggt atgcagaaca
    36001 ttgaacaggg gctggaggct tgccttgccg gtctgctggt ttctattctg ttcatgatca
    36061 tcttctataa gaagtttggt ctgattgcga ccagtgctct gattgccaac ttgatcttaa
    36121 tcgtcggcat tatgtcgctg ttgccaggcg caacgctgag tatgccaggt atcgcgggta
    36181 tcgtcttaac ccttgcggtg gcggtcgatg cgaacgtact gatcaacgaa cgtattaaag
    36241 aagagttgag caacggacgt actgttcaac aggcaattga tgaaggttat cgtggcgcat
    36301 tcagttctat cttcgatgcg aacatcacca cgctgattaa agtcatcatc ctgtacgcag
    36361 tgggtaccgg ggcaattaaa gggttcgcga ttactaccgg tatcggtgtg gcgacgtcga
    36421 tgtttaccgc gattgtcggt acgcgtgcca tcgtaaacct gctatatggc ggcaagcgcg
    36481 tcaagaagct gtcaatctga ggagtgcgat gtggcacagg aatatactgt tgaacaacta
    36541 aaccacggcc gtaaagtcta tgactttatg cgctgggact actgggcttt cggcatctct
    36601 ggtctgctgt taatcgccgc tatcgttatt atgggcgtgc gcggctttaa ctgggggctg
    36661 gatttcaccg gtggtacggt tatcgaaatt acgctcgaaa aaccggctga aattgacgta
    36721 atgcgtgatg cattgcaaaa agccggtttt gaagagccaa tgctgcaaaa ctttggtagc
    36781 agccacgaca tcatggtccg tatgccgcct gctgaaggcg aaaccggcgg tcaggtgctg
    36841 ggcagccagg ttctgaaggt gattaacgaa tccaccaatc agaatgcagc agtgaagcgt
    36901 attgagttcg tcggtccgag cgtgggggca gaccttacgc aaaccggtgc gatggcgttg
    36961 atggcggcgc tgctgtctat ccttgtgtac gtaggttttc gctttgagtg gcgactggcg
    37021 gcaggggttg ttattgcgct ggcgcacgac gttatcatta cgctgggtat tttgtcgtta
    37081 ttccatatcg agattgacct gaccattgtg gcatcgttga tgtcggttat cggttactcg
    37141 cttaacgaca gtatcgtggt atcggaccgt attcgtgaaa acttccgcaa gatccgtcgc
    37201 ggtacgcctt acgaaatctt taacgtgtcc ttgacccaga cgctgcaccg taccttgatc
    37261 acatccggta ctaccttgat ggttatcctg atgctgtacc tcttcggtgg tccggtactg
    37321 gaaggcttct cgctgaccat gcttatcggt gtttccatcg gtactgcatc ttccatctat
    37381 gtggcatctg cgttggctct gaaactgggt atgaagcgcg aacacatgtt gcagcagaaa
    37441 gtggaaaaag aaggggcgga tcagccgtca attctgccgt aatcaagttc ccgttgatgt
    37501 tgaaaatccc ggtcagaaga tcgggatttt ttttgatgta tggagattgc gaaaatcccc
    37561 gcatcttggg aaactgcgcg taaccctaca tttcatccag gtaactcttc atggctatca
    37621 tcccaaaaaa ctatgcgcgg ttagaaagcg gctatcgcga aaaagcatta aaaatatatc
    37681 cgtgggtctg cggtcgctgt tcccgcgagt ttgtttattc caacctgcgt gaacttaccg
    37741 ttcaccacat tgatcacgac cataccaata acccggaaga tggcagtaac tgggaattgc
    37801 tttgtctcta ttgccacgat catgagcatt cgaaatatac cgaagcggat cagtatggta
    37861 cgaccgttat cgcaggggaa gatgcgcaga aagatgtcgg tgaagcgaag tacaacccat
    37921 tcgctgacct gaaagcgatg atgaacaaga agaagtgatt aaaacgtaaa attgcctgat
    37981 gcgctacgct tatcaggcct acgttatttc agcaatatat tgaatttacg tgcttttgtg
    38041 ggccggacaa agcgtttacg ccgcatccgg catgaacaaa gctcacgttg ttaacaatca
    38101 gaaatgccgg gaataaatcc cggcattttc ataatcagaa gttgtaacct actaccaggt
    38161 aaccacccca gccggtagag cgaacgttga agttgccgtt gccgaagttc agttctgcat
    38221 cgtcgttcca ctgaccaccg tcgtgccagt aacgagctac gacagagtag tgccagtgat
    38281 cgtagttcag agccagaata tggctggaag cgatagagtt attagtacgg gtcttaatac
    38341 cgttgattgc gttaccgctg tcatccccta aatcggaacc ccagtcgaag ttggtgaagc
    38401 cgatgtagct cagctgaccg ccccacagat cggtaatcgg cacaaagtat ttaattttga
    38461 aacggtaacc gtcccactcg ttttcgttcg ctgcgccata gttctgccac tggtatttcg
    38521 catagacgtt catggacagg ctcatcggca gaccagtatc gatgtcggta cccagaccca
    38581 tgtaccaggt gctctggcga ccatttttat tacgacccat gtcgtaaatg tagttgttcg
    38641 cgaagtacca ctctttgaac ggaccgaagt taaggtcagt attggtcagc ttgtcgatgg
    38701 agaaacgtgg ttcgatttcc ataaacagcg gagaaccgtg gttccagata cctttagcat
    38761 cggagttacc gccgaagaat accggcgcat ccgcataacc atagaagtcg aaccagtctt
    38821 ttttagcgaa tgcttcgtac tcaaggtagg tatcgttgcg gatctgcggt ccgaaacggg
    38881 tgtgatagct tccgacaacg ttaacgctct ggtgccacca gtcggaaaga tactgcggtt
    38941 tgtcgttttc agctgcgttg acagtaaaag acgaagagag cgccagtacc gcaccggctg
    39001 ccagtaatgt ttttttcata tgtatgccac tgtttgaaaa tcccttgcgg gagtgaaaaa
    39061 ggcgcaaatt gcgtttctaa atatttcgta tacagaggag cctattataa aaatcattgc
    39121 tcacaaaaat atgtttcgtt tcacagttct atcatttacg taatcgattg cgttcacgtt
    39181 tacacacatt cgggcgggga ttgtactgac tttcgccctt gttgcaaact tttactatca
    39241 atccaggtga tgtaagcaga gtaacaaaat gacggggaaa gatgttgcgg gacgcacaaa
    39301 atcgtcccga aatacgttta ctgcataaca ggttgaatgt cgtggatacg tacaaagcct
    39361 aactgatcca cagaaatccc ctttaattgt aacggaatat caacatcgct gggggcgagg
    39421 acgctggcag gggcattaat caattgattc tgcacattga tttcctgaaa gttgtctatt
    39481 gttccctgaa tttgcccata ttcaacagtt ccgctgaatg cgggcaaggg atcattcgac
    39541 tcaccctgaa tacgtagtgt aagggtggtg ccatctgtat caggcgtaat attcaccagc
    39601 gacatacgta aagtaccgat ctggctttcc agtcttgccg gtgttttcgc tccaggcaga
    39661 aggtaacccc cactgctgga ttttgcattc agtctgtttt gctgagtgat tttgactgtt
    39721 tcttgattga gttgcgtcat ctcctggttc agcgtgctga cgctgtgttt catctgtcgc
    39781 acttcacttt gctgcacaca ggcgctaagg ctgaacaggc ttcccagcag gagcagtcga
    39841 aaaacgttag tattcatagg tcgtcccttt ctcgtcgcaa ttagcctaat ggtagagggt
    39901 aaaagcagcg aaagcatcga tcctttatct caaaagcgtt gcgcctttgt tgtatcgtca
    39961 gttcagggta aaatagattt ccgttaacca cctggtcagg acgccgtatg cattgcccat
    40021 tctgtttcgc cgtggacact aaggtaattg actctcgtct cgtgggcgag ggttcatccg
    40081 tacgccgccg tcggcagtgt ctggtgtgta atgaacgttt caccaccttt gaagtggcgg
    40141 agctggttat gccgcgtgtt gtaaaaagca acgacgtgcg tgaaccgttt aatgaagaga
    40201 aattgcgtag cggaatgctg cgggcgctgg aaaaacgtcc ggtgagttcc gatgacgttg
    40261 aaatggcaat caatcatatt aaatcgcagc tgcgcgccac cggtgagcgc gaagtaccga
    40321 gcaagatgat tggcaatctg gtgatggagc aattgaaaaa gctcgataaa gtcgcctata
    40381 tccgttttgc ctctgtctac cgcagtttcg aagatatcaa agaatttggc gaagagatcg
    40441 cgcgcctgga ggactaagcc gtgcaggacg agtattacat ggcgcgggcg ctaaagctgg
    40501 cgcaacgagg acgttttacc acccacccca acccaaatgt cgggtgcgtc attgtcaaag
    40561 atggcgaaat tgtcggtgaa ggttaccatc aacgagcggg tgaaccacat gccgaagtac
    40621 acgcgttgcg tatggcgggt gaaaaagcca aaggtgcaac ggcgtatgtg acactcgaac
    40681 cttgcagcca tcacggccgt acgccgcctt gctgtgatgc tttaattgcc gcaggtgtgg
    40741 cgcgcgtggt tgcctcgatg caagatccta acccgcaggt cgctgggcgt ggactttacc
    40801 gtctgcaaca ggctggcatt gacgtcagcc acggcctgat gatgagtgaa gccgagcaat
    40861 tgaataaagg ctttctcaag cggatgcgca ccggctttcc ttatattcag ttaaaacttg
    40921 gcgcatcgct tgatggtcgc acggcgatgg cgagcggcga aagccagtgg atcacttcgc
    40981 cccaggcgcg gcgtgatgta caacgactgc gcgcgcaaag tcatgccatt ttaaccagca
    41041 gcgccacggt gctggcggat gatcctgcct taacggtgcg ttggtccgaa ctggatgaac
    41101 aaactcaggc gctctacccg caacaaaatc tccgccagcc ggtacgtatt gtgattgata
    41161 gccaaaatcg cgtgacgccg gaacatcaca ttgtgcagca gcccggcgaa acctggttcg
    41221 cgcgtacgca ggaagattct cgtgagtggc cggaaacggt gcgtaccttg ctgattccag
    41281 agcataaagg tcatctggat ctggttgtac tgatgatgca actgggtaaa cagcagatta
    41341 acagcatctg gggggaagcg gggccaacgc tcgctggcgc attgctgcag gcgggtttag
    41401 tcgatgagct gattgtctat atcgcaccta aactattagg cagcgacgcc cgtggattat
    41461 gcaccctgcc agggcttgag aaattagccg acgcccccca atttaaattc aaagagatac
    41521 gtcatgtagg cccggatgtt tgcctgcatt tagtgggtgc atgatctccc ggctcgaaag
    41581 ggaagcagcg cacgaaatat tatgctaaaa tccgcccccc tgcggggcca tactcgaacc
    41641 cgaaggaaga aaatgaacat tattgaagct aacgttgcta ccccggacgc tcgcgtcgcc
    41701 atcaccatcg cgcgtttcaa caactttatc aatgacagcc tgctggaagg tgcaattgac
    41761 gcactgaaac gtatcggtca ggtaaaagat gaaaacatta ccgttgtttg ggtgcctggt
    41821 gcctatgagc tgccgctggc ggcgggtgca ctggctaaaa ccggtaaata cgacgcggtg
    41881 attgcgctgg gtacggttat tcgtggtggc actgcccatt ttgaatatgt cgctggtggt
    41941 gcaagcaacg gcctggcgca tgttgcccag gacagcgaaa ttccggttgc ttttggggtt
    42001 ctgaccactg aaagcattga acaagcgatc gaacgtgctg gcaccaaagc tggcaacaaa
    42061 ggtgcagaag ctgcactgac cgcgcttgaa atgattaatg tattgaaagc catcaaggcc
    42121 tgaaattagt aaggggaaat ccgtgaaacc tgctgctcgt cgccgcgctc gtgagtgtgc
    42181 cgtccaggcg ctctactcct ggcagttgtc ccagaacgac atcgctgatg ttgaatacca
    42241 gttcctggct gaacaggatg taaaagacgt tgacgtcctg tacttccgtg agctgctggc
    42301 cggggtggcg actaataccg catacctcga cggactgatg aagccatacc tgtcccgtct
    42361 gctggaagaa ctgggacagg tagaaaaagc agtactgcgc attgcgctgt acgaactgtc
    42421 taaacgtagc gatgtgccat acaaagtggc cattaacgaa gcgatcgaac tggcgaaatc
    42481 gttcggcgca gaagacagcc ataagttcgt caacggtgta ctcgataaag cagcacctgt
    42541 gattcgccct aacaaaaagt aatatccagg ccggtagatt cacggaagac cgttccatga
    42601 tcgccggcct tttctttttt acctgctgag gcataacgta tggcatgtgg cgagttctcc
    42661 ctgattgccc gttattttga ccgtgtaaga agttctcgtc ttgatgtcga actgggcatc
    42721 ggcgacgatt gcgcacttct caatatcccc gagaaacaga ccctggcgat cagcactgat
    42781 acgctggtgg cgggcaacca cttcctccct gatatcgatc ctgctgatct ggcgtataaa
    42841 gcactggcgg tgaacctaag cgatctggca gcgatggggg ccgatccggc ctggctgacg
    42901 ctggcattaa ccttgccgga cgtagacgaa gcgtggcttg agtccttcag cgacagtttg
    42961 tttgatcttc tcaattatta cgatatgcaa ctcattggcg gcgataccac gcgtgggcca
    43021 ttatcaatga cgttgggtat ccacggcttt gttccgatgg gacgagcctt aacgcgctct
    43081 ggggcgaaac cgggtgactg gatctatgtg accggtacac cgggcgatag cgccgccggg
    43141 ctggcgattt tgcaaaaccg tttgcaggtt gccgatgcta aagatgcgga ctacttgatc
    43201 aaacgtcatc tccgtccatc gccgcgtatt ttacaggggc aggcactgcg cgatctggca
    43261 aattcagcta tcgatctctc tgacggtctg atttccgatc tcgggcatat cgtgaaagcc
    43321 agcgactgcg gcgtacgtat tgacctggca ttgctgccgt tttctgatgc gctttctcgc
    43381 catgttgaac cggaacaggc gctgcgctgg gcgctctctg gcggtgaaga ttacgagttg
    43441 tgtttcacgg tgccggaact gaaccgtgga gcgctggatg tggctctcgg acacctgggc
    43501 gtaccgttta cctgtatcgg gcaaatgacc gccgatatcg aagggctttg ttttattcgt
    43561 gacggtgaac ctgttacgtt agactggaaa ggatatgacc attttgccac gccataaaga
    43621 tgtcgcgaaa agtcgcctga agatgagtaa tccgtggcat ctacttgctg tcggattcgg
    43681 aagtggatta agcccgatcg ttcctgggac gatgggctcg ctggcagcga ttccgttctg
    43741 gtatctgatg acctttttgc cctggcagct ctactcgctg gtggtgatgc tggggatctg
    43801 tatcggcgtc tatctttgtc atcagaccgc aaaagatatg ggcgtgcacg atcacggcag
    43861 cattgtctgg gacgaattta ttggtatgtg gatcacgctc atggcgctgc cgaccaatga
    43921 ctggcagtgg gttgccgccg ggtttgtgat tttccgtatt ctggatatgt ggaaaccgtg
    43981 gccgatccgc tggtttgatc gcaatgtgca tggcggcatg gggatcatga tagatgatat
    44041 tgtcgccggg gtgatctccg ctggcatcct gtactttatt ggtcatcact ggccgctggg
    44101 cattctgtcg tagttgtgca ccgatgcctg atgtgacgct tgttgcgtct catcaggcct
    44161 ggactcttat ttaaatccta ctaccggatg cggtttatac ggcgtttcca gttcggcaat
    44221 ctgttccggc ttcaaagtga tatccaccgc gttcaatagc tcatcaagct gttcttcccg
    44281 cgatgtaccg ataatcggtg cggcaatgcc cggtttactc aacaaccaag ccagcgcaac
    44341 ttgtgctcgt gttgcaccga gttcttcact gacgcccgtt aaccgctctg cgatctgcgc
    44401 gtcattttca tcgctttctt tatagagatt tttccccacc tcatcagaca ccagtcgtgc
    44461 ggtagtttct ccccacggac gcgtcagtcg cccccgcgcc agcgggctcc acggaatcac
    44521 cgccacgccc tcctgataaa acagtggcag catctcgcgc tcttcttcgc gataaatcag
    44581 attgtagtga tcctgcatac tgacaaactg cgcccagccg tgctgttttt ggagttccag
    44641 tgcctgagca aactgcgaag cgtgcattga tgacgcgccg atataacgcg ctttcccggc
    44701 ttttaccacg tcgttcaggg cttccagcgt ctcttcgatc ggcgtgttgt aatcccagcg
    44761 atgaatttgc aggatatcga cataatccat gccgagacgt cgcaggctgt cgtcgataga
    44821 gcgcaaaatt tgtgcacggg ataatccttc cggtaaatca ccaacgcgat ggaacacttt
    44881 ggtggcaacg accacgtctt cacgacgggc gaaatcccgc agtgcgcgac caacgatctc
    44941 ttcgctgcta ccatcggaat agctattggc ggtatcaaag aaatttatgc cgccttccag
    45001 tgcgcgttta attatgggac ggctgctctc ttccggcagt gtccatgcgt gattaccacg
    45061 atctggctcg ccaaaggtca tacagccgag gcaaagtcgg gaaacgcgaa ggtcggtttt
    45121 tcctaagggg ttgtattgca tgctgccact cctgccatga ataatttctt aagcatagca
    45181 ggagtggagt agggattatg ccagccaggc cttgattttg gcttccatac cggcggcatc
    45241 gaggccgagt tcggcgcgca tttcttcctg cgttccttgc ggaataaaga agtcaggcag
    45301 gccaatgttc agcacgggga ctggtttacg atgggccatt agcacttcgt tcactccgct
    45361 gcctgcaccg cccataatgg cgttttcttc tacggtaacc agtgcttcat gactggcggc
    45421 catttccaga attaacgctt catcaagcgg tttcacaaaa cgcatatcga ccagcgtggc
    45481 gttcagcgat tcggcgactt tcgccgcttc tggcatcagc gtaccaaagt taaggatcgc
    45541 cagtttctcg ccacgacgct tcacaatgcc tttgccaatt ggtagttttt ccagcggcgt
    45601 cagttccacg ccgaccgcgt tgccacgcgg gtagcgcacc gctgacgggc catcgttata
    45661 gtgatagccg gtatagagca tctggcgaca ttcgttttca tcgctcgggg tcataatgac
    45721 catttccggt atgcagcgca ggtaagagag atcaaaagca ccctgatggg tttgaccgtc
    45781 agcaccaaca atgcccgcgc ggtcgatggc gaacaggacc ggaagctttt gaattgccac
    45841 gtcatgcagc acctgatcat aggcgcgttg caggaaagtg gagtagatcg cgacaatggg
    45901 tttgtaccca ccaatcgcca gaccggcggc aaaggtcact gcgtgttgct cggcaattgc
    45961 cacgtcgaag tagcgatccg ggaatttacg agaaaactcg accatgccgg aaccttcacg
    46021 catcgccgga gtaatcgcca tcagcttgtt atctttcgcc gccgtttcgc acaaccagtc
    46081 gccaaagatt tttgaatagc tcggtaaacc gccgctactt ttcggcaaac aaccgctgga
    46141 gggatcaaat ttaggcacgg cgtggaaagt gattgggtct ttttctgccg gttcataacc
    46201 acgacctttt ttggtcatga tatgcaggaa ctgcgggcct ttcaggtcac gcatgttctt
    46261 cagcgtggtg ataagcccca gcacatcgtg accgtcaacc gggccgatgt agttaaagcc
    46321 cagctcttca aacaacgtgc cagggactac catgccttta atatgttctt cggtacgttt
    46381 gagcagctct ttaatgggtg gcacgccaga gaaaactttt ttcccgcctt cgcgcagtga
    46441 agagtaaagc ttaccggaaa gcagctgcgc cagatggttg ttgagcgcgc cgacattttc
    46501 ggaaatcgac atttcgttgt cgttgaggac caccagcata tcaggacgga tatcgcccgc
    46561 gtgattcatc gcttcaaacg ccatgcctgc ggtaatcgcg ccatcgccga ttacacagac
    46621 ggtacggcga tttttgcctt ctttctcggc agcaaccgca ataccaattc cggcactgat
    46681 ggaggttgat gaatgcccga cgcttaatac gtcatattcg ctttcgccgc gccacgggaa
    46741 cgggtgcagg ccgcctttct gacggatggt gccgattttg tcgcggcgtc cggtcaaaat
    46801 tttatgcgga taagcctgat gccccacatc ccaaatcaat tggtcaaacg gggtgttgta
    46861 gacatagtgc agcgccacgg tcagttcgac cgtgcccagc ccggaggcga agtgcccgct
    46921 ggaacggctc acgctgtcga gtaaatagcg gcgcagttcg tcgcagagtt tcggtaaact
    46981 ctctttcggc aacagtcgta actcctgggt ggagtcgacc agtgccaggg tcgggtattt
    47041 ggcaatatca aaactcatca ggggcctatt aatacttatt gtttatttat tacgctggat
    47101 gatgtagtcc gctagcgctt ccagtgccga ggtatcgggt gactgttcag ccagttgttt
    47161 cagcgactga cgggcatcgt cgatcagatc ccgggctttc ttccgggctt gctcaagacc
    47221 cagaagtgca gggtaggtac ttttaccaag ttgctggtcg gcaccctggc gttttcccaa
    47281 cgttgcagta tctcctacca catccaggat gtcatcctga acctggaagg caaggccgat
    47341 gctctctgcg tacttgtcga gtactggcag agcacgacgc cctttatctc cggcgcttaa
    47401 tgcaccaagg cgaacggcgg cgcgaatcaa tgcgccggtt ttatgacgat gaatacgctc
    47461 aagcgcgtcc agaggtacgt gtttgccttc cgcgtctaaa tctaatgcct gaccaccgca
    47521 cattccggca ataccgctgg cgctcgccag ttcagaaatc atcgaaattc tgtcgcgatc
    47581 cgacacttcc ggcatatcag catcgcttaa aatcgagaac gccagcgttt gtaaagcgtc
    47641 gccagcgaga atcgcgtttg cttcgccaaa cttcacatgg caggtcggca aaccgcgacg
    47701 cagatcgtca tcatccatcg ccggtaaatc atcatgaatt aatgagtaag cgtggataca
    47761 ctctacggcg gcagcgggtg cgtccagcgt gtttgtgcta acgccaaaca tatgaccggt
    47821 ggcataaacc aggaagggtc gcagacgctt accacctaat aatgcaccat actgcatggt
    47881 ttcgaccacg ggagtgttct gaaagggcag tggggcgata aaacggctca gcgcctggtt
    47941 ggcctgctta acgcaggctt cgagttgctg cggaaagtcc attactcatt gtccggtgta
    48001 aaaggggtta gagaggcgtc ttcattgtca gacagcagaa tttgtacgcg ctgttcggct
    48061 tgttgtaatt tggcctgccc ctgacgtgcc agctgcacgc cgcgttcgaa ctcgttcagc
    48121 gcctcttcca gcggcaggtc gccactttcc agacgggtta caatctgttc cagctcgctc
    48181 agcgcctttt caaagctggc gggagcctca tttttcttcg gcataatgaa tgtctgactc
    48241 tcaatatttt tcgccccgtc atggtaacgg actcagggca aatagcaaat aacgcgcaat
    48301 ggtaaggtga tgtgcgcagc aaagcgatgt tagtggtata cttccgcgcc tggatgcagc
    48361 cgcaggtgtg ggctgctgta tttttcccta tacaagtcgc ttaaggcttg ccaacgaacc
    48421 attgccgcca tgaagtttat cattaaattg ttcccggaaa tcaccatcaa aagccaatct
    48481 gtgcgcttgc gctttataaa aatccttacc gggaacattc gtaacgtttt aaagcactat
    48541 gatgagacgc tcgctgtcgt ccgccactgg gataacatcg aagttcgtgc aaaagatgaa
    48601 aaccagcgtc tggctattcg cgacgccctg acccgtattc cgggtatcca ccatattctc
    48661 gaagtcgaag acgtgccgtt taccgacatg cacgatattt ttgagaaagc gttggttcag
    48721 tatcgcgatc agctggaagg caaaaccttc tgcgtacgcg tgaagcgccg tggcaaacat
    48781 gattttagct cgattgatgt ggaacgttac gtcggcggcg gtttaaatca gcatattgag
    48841 tccgcgcgcg tgaagctgac caatccggaa gtgactgtcc atctggaagt ggaagacgat
    48901 cgtctcctgc tgattaaagg ccgctacgaa ggtattggcg gtttcccgat cggcacccag
    48961 gaagatgtgc tgtcgctcat ttccggtggt ttcgactccg gtgtttccag ttatatgttg
    49021 atgcgtcgcg gctgccgcgt gcattactgt ttctttaacc ttggcggcgc ggcgcatgaa
    49081 attggcgtgc gtcaggtggc gcattatctg tggaaccgtt ttggcagctc ccaccgcgtg
    49141 cgttttgtcg ctattaattt cgaaccggtc gtcggggaaa ttctcgagaa aatcgacgac
    49201 ggtcagatgg gcgttatcct caaacgtatg atggtgcgtg ccgcgtctaa agtggctgaa
    49261 cgttacggcg tacaggcgct ggtcaccggc gaagcgctcg gccaggtgtc cagtcagacg
    49321 ctgaccaacc tgcgcctgat tgataacgtc tccgatacgc tgatcctgcg tccgctgatt
    49381 tcttacgaca aagagcacat catcaacctg gctcgccaga ttggcaccga agactttgct
    49441 cgcacgatgc cggaatattg cggtgttatc tccaaaagcc cgacggtgaa agcggttaag
    49501 tcgaagattg aagcggaaga agagaaattc gacttcagca ttctcgataa agtggttgaa
    49561 gaagcgaata acgttgatat ccgcgaaatc gccctgcaga ccgagcagga agtggtggaa
    49621 gtggaaaccg tcaatggctt cggcccgaac gacgtgatcc tcgatatccg ttctatcgat
    49681 gaacaggaag ataagccact gaaagtcgaa gggattgatg tagtttctct gccgttctat
    49741 aaactgagca ccaagtttgg cgatctcgac cagaacagaa cctggctact gtggtgtgag
    49801 cgcggggtga tgagccgtct gcaggcgctc tatctgcgcg agcagggctt taacaatgtg
    49861 aaggtatatc gcccgtaatt tgtggttgta acgtcgcatc tggtcagatg cgacgcttac
    49921 cgcatccgac actactcgta ataattataa atccctgccg ccatcaccag ttgtgatgcc
    49981 acttcatggg ctttctcgcg cccaacctgc aggtcgataa ttttcagacc aaagtcgata
    50041 gctgtccccg gcccctggct ggtcagcaat tttacccgtg catcccagac gacgcgcttg
    50101 tcctgccatt gttcagcggg aattttgtct ttcagcgtcg ggaagccggt catattacca
    50161 atcgggaaga tatcgtgcgg caccagcacg gtggctggcg cggcgcaaat agccgcgacg
    50221 atacgcccgg aacggtggaa ctgtttaacg gtttcaacca gcagagtgct atcgcgaaaa
    50281 cactctgcgc ctttaatgcc accaggcagt acgatcacgt catattcgcc atcagccacc
    50341 tcgaccagcg gcgcatccgc cagcagcttc acgccgcgcg agcaggtaat tgccaggttg
    50401 ccatcgctgg ctacgctggc agtggtgact ttgataccgc cgcgaaccaa gaggtcgatc
    50461 gtggtgacgg cttcagtctc ttcactacca ggggcgaggc aaaccagtgc cgatgcgctc
    50521 atattcactc tcctttcttt ttaccatttc aaacaggcgg gtgttttccg gtacggcaat
    50581 cccatgcgcg cgggcgcggc gtaagagaaa accattgata tagtcgattt cggtgtggcg
    50641 cagcgcgcgg atatcctgca acatcgacga gatattttcc gctgtggcat caatcacctg
    50701 catcacgtaa tcacgcaaat cttccgctga agtatgatgc ccttcgcgtt cgatcactgc
    50761 cgcgacttct tcgcatattt gcataatttc ttgcggatga tgacgtaatt caccgttcgg
    50821 gcaattccag atggcggtca gtggattaat cacgcagttg actgccagct tgcgccacag
    50881 ctcggcgcga atattgttat gccaggcaac gtcaggcaac acggtttgta aaatatccgc
    50941 cagataactg taatcgccat cctgttgccg tgccggacca atatgcgtga taccgtttgc
    51001 cacatgaata atgacattgc cgtcgcggcg ggcggcatgg gtggtggtgc ccatcagtaa
    51061 tggctgctga atattttgca actcttcgat ggtgcccatg ccgttgtgaa ttaacagtat
    51121 tggcgtggtt acaggcagtg tggacgcgag gcttttgacg gcatcggaaa cctgccatgc
    51181 tttcagcgtc accaggagca gatcgctggt ggcgagaaaa tcgggatcgt tggcggtcag
    51241 cgattcgtta aatatcgaac catctgtctc aaccagattc gcactacaat aaggttgcgg
    51301 tacgcgcagc cagccctgaa cttcatgacc ctgtttgcaa agtgctgtaa gccataattg
    51361 ccctaaggca ccgcatccca atacggtaat tttcattgtt cctcctcacc cgcaaccact
    51421 ccgggtgttc aataaggcta tcccttaatt gtgcatgctg ttgcgactat gcacaattaa
    51481 gggatacgtc ctggtgcagg actgtcggtt atttaacttt gcgggtatta tgcttcgcat
    51541 caaaaatgaa gggagaggaa aagatgccat ctttcgatat tgtctctgaa gttgatcttc
    51601 aggaagcacg taacgcggtc gataacgcga gccgcgaagt ggagtcccgt tttgacttcc
    51661 gtaacgttga agcctcattt gagctgaacg acgccagcaa aaccatcaaa gtgttgagcg
    51721 agtccgattt ccaggtcaat cagttgctgg atattctgcg tgccaagctg ctgaagcgcg
    51781 gcattgaagg cagttcactg gatgtaccgg aaaatatcgt tcatagcggt aaaacctggt
    51841 ttgtggaagc gaaactgaag cagggcattg agagcgcgac ccagaaaaaa atcgtcaaga
    51901 tgatcaaaga cagcaaactg aaagtgcagg cgcaaattca gggcgatgag atccgcgtga
    51961 ctggcaaatc tcgtgatgat ttgcaggctg tcatggcgat ggtgcgcggt ggcgatctgg
    52021 gtcagccgtt ccagttcaaa aacttccgcg attaatcgcg acaacctgcg ctttgttcat
    52081 gtcagatgcg gcatgaacgc ctggttcggc ctacaaaggc ttgtaaatca ggagcttagt
    52141 aggcctgatg agcgtagcgc agcaggcaat ttcgcctcgc ttttatgcct ggcgaattgc
    52201 ctgttcaaca tcaaagcgat tcgtcacttt gctgtcgatt ttcacataag ctgaatgttc
    52261 ttcttctgca atcaacactt ctttgatgcc ttcagtttct agcaaacgca cttttaacgc
    52321 ctcgtttgcg gcaatgttcg ccgggatttc aatgcgcaaa ctgctgacat acgccggttc
    52381 tttcatggta ctggcgactg ccagccacac tgcggccagc attgcgccag cgagaaatac
    52441 gccctgaccg tcaaacatgc cgtcaatcca gccgcccagt gaaccgccaa tcgccacgcc
    52501 aagaaactgg ctggtggagt aaacacccat cgctgtacct ttgtaacctg ctggcgactc
    52561 tttactgata agtgaaggca gaagggcttc catcaaatta aacgctacaa agaaaagctg
    52621 cacgccgacc accagttgcc agaactgcgt ttgcgcgttc cacaacacaa tttccgcaac
    52681 cacgatcaat ccgacgcaga agacaaagac ttgcttcatt ttgcgcttaa cttcagcgta
    52741 gataatgaaa ggcacgaccg agccaaaggc gattagcatt gtcgccagat agaccttcca
    52801 gtgttcagcc gccgggaacc ctgcatcagc cagttgtccg ggcagggcaa caaacgtcga
    52861 catcagcaaa atatgcagac acataatgcc aaagttgagt ttcagcagcc gcggttccgc
    52921 cagcacttta ctgaaactgc ctttcaccat tccggactca cgattaagta cgtgagtgct
    52981 actgttgggc acaacccaaa tggtcaacgc aatgccggtc gttgccagaa tagcgatcat
    53041 ccagaacagc gcgtgcagcc caagtttgtg agtgatgatc gggccaagca ccatcgcaat
    53101 ggcaaaggta atgccaaagc tcacgccgat aaacgccatt gctttggtgc ggttttgttc
    53161 gcgcgtgaga tcggaaagca gcgccataac ggcagcggca atcgcaccgg agccttgtag
    53221 cgcccggcct agaataattc cccagatgga gtcagagagc gcagcgataa cgctaccggc
    53281 ggcaaacacc gccagcccac cgacaattaa tggtttgcga ccaatacggt cggaaagcag
    53341 gccaaacgga atctgaaaaa cggcctgagt cagaccataa ataccaatgg caataccgat
    53401 taatgcttcg ctggcacctt gcagagccat gccatacgtg gtcagaaccg gcagaaccat
    53461 aaacatgccc agcatgcgca acgagaatac ggtccctaaa ccccaggtcg cgcgcctctc
    53521 acctggcgtc attttataat cgttcattac cacctctgtt ttaaattcgc gactagtgta
    53581 aagcggcaaa cgaatggggt aaacatgcgg attttttagt aaatatttcg agagggatat
    53641 gtttctaatg ctaagaaaaa aggtgccgta gcaccttttt aatagagagg ttttgttacc
    53701 acacagcagc cagcagcgta tgcgagtccg gtaccataaa atcaacggac atcatcaccg
    53761 agagggcagt gatggcgatg atagagaagc cgaacagctt gcgcgcccag attctgtcat
    53821 cagcaacttt ataaccgcgc agagccatac ctaaccacca gacgctaacc gccgcggcga
    53881 ccaccagata tttatatcca gcgtaaccgc caagagagag catcagcgtg gcaacggcaa
    53941 aggcgatgat atacagcgtg atgtgattct tcgccaccga aatgcctttt accactggca
    54001 ataccggaat gtttgccgcc tggtaatcct taaagcggaa aatggcgatg gcataggagt
    54061 gaggcatctg ccacaggctg aagatagcca gcaggatcgc tgcgccgcta tcgaactcgc
    54121 cggttactgc acagtagcca atcaccggcg gcgcagcgcc ggagagcgaa ccaatcaatg
    54181 tgccgtagac tgagtggcgt ttcatgtaca ggctatagac gccgacataa accacaaagc
    54241 ccatcacccc cagccagcag gccagcggat tcgcgccaaa ccacagcagc ataaagccag
    54301 caatacccag caaggtggcg tacaccagcg agacagcagg agagatcagg cctttaacca
    54361 gcacccgatt cttcgtcctt tccatctttc tgtcgatatc cctgtcgatg tagttgttaa
    54421 acacacaacc cgacgccaca accagtgaca ccccaaccag cgtgtagata aacaggggat
    54481 aatcaatgct gccctttgag gccagcagga atcccccaat caccgagatc aggttgccaa
    54541 agatgatgcc tggtttcgtt acttgcaggt attgcttaaa catcataacc gccgctctta
    54601 gtgcatcatc atgttgtagt tgaggttcca cataatccag atggagccta caaccaggat
    54661 agcgatgatt agcacggtga agacaaacgc cgtcatgttc cagccttcat ctgatttggt
    54721 attcatgtgc aggaagcaca ccagatgcac cagaacctgt accactgcca ttgccaggat
    54781 tgttcccaga attacggccg gagaggcagc ccctgtcatc accatccaga acggaatcac
    54841 cgtcagaatg atcgacagga taaagcctgt catgtaggtt tttacgctgc catgggacgc
    54901 gccgctgtga tcggtagaat gactcattac atcgccccca tcagataaac aacagtgaac
    54961 acacagatcc aaaccacatc caggaagtgc cagaacaggc tcaggcacat gatgcgggta
    55021 cggttagtgc tggtcaggcc gcgacgagcg atttgtacca tcagcaccgc catccagata
    55081 agaccggaag tgacgtgcag accgtgcgtg ccgaccagcg caaagaacgc tgacaggaag
    55141 ccgctgcgat ccggacccat gccgttaaca atcaggtgat ggaattcgta gatttccatc
    55201 ccgataaatc cggcaccaaa caaacaggtc aacgccagcc aggagataac ctggcttttg
    55261 ttgtttttgt acatggcgat agccgccatg ccgtaggtga tggagctgaa caacagcaag
    55321 aaagtttcaa ccagaacgaa cggcagttcg aaaatgtcct tacctgtcgg gccgcctgcg
    55381 gtgccgttca ccagaacggc ataggtagca aacaagattg agaacagaat gcagtcgctc
    55441 atcaggtaga tccaaaatcc gaagattttg gttccgcctg catcgtggtg cccgtgttcg
    55501 tgcgcgtggg cagtcgcgtg cgtcaaagta tcagttgcca tttttcagcc ctgccttagt
    55561 aatctcatcg aaatgctggt tttccagttt ttcgatttct gccaccggca cgtagtaatc
    55621 cacgtcctcg tcgaagcttt tcacgatcca ggtgatgatc atgcctgcga agccaacaat
    55681 cgccagccac cagatatgcc agatcatggc gaaaccgaag atggtggaga aagctgcaat
    55741 gacgatacct gcaccgctgt ttttcggcat atgaatttct tcatagtggt caggcttttt
    55801 atacgcttcg cctttctctt tcatttccca gaatgcatca cgctcgtgaa cgtgcggcac
    55861 aacggcaaag ttatagaacg gaggcgggga agagtttgcc cactccagcg tacggccacc
    55921 ccacgggtcg ccagtcaggt cacggttctg gtcgcggtcg cgaatagaaa cgtacatctg
    55981 aataacgagg cagagaatac ccagcgcaat cagtactgca ccgctagctg caatcatcag
    56041 catggtgtgg aactgcgggt caatctgctg gctcaaacga cgggtcatgc ccatgaagcc
    56101 cagtgcatac agcggcataa aggcaacgaa gaagccgatg atccagaacc agaacgcgcg
    56161 tttaccccag gtttcattca gtttgaaacc gaacgcttta ggccaccagt aggtcatccc
    56221 tgcgaagcag ccgaagacca cgccgccgat gataacgtta tggaagtgcg caatcaggaa
    56281 caggctgtta tgcagaacga agtccgcgcc cggtactgcc agcagcacgc cagtcatccc
    56341 gcccaccgag aaagtgacga taaaaccgat ggtccacagc atcgcagaat ggaacacgat
    56401 acggccctga tacatggtga acagccagtt gaagatcttc accccggtcg ggatggcgat
    56461 aatcattgtg gtgataccaa agaaggcgtt tacgttcgcg cccgcaccca tcgtaaagaa
    56521 gtggtgcagc caaacgatga acgacagcac ggtgatacag acggttgccc ataccagcga
    56581 ggtataacca aacagacgtt tacgcgagaa ggttgccgca atttcggaga acacaccgaa
    56641 aacaggcagg atcaggatgt aaacttccgg gtggccccag gcccaaatca ggttgatgta
    56701 catcatcatg ttgccaccca tatcgttggt aaagaaatgg gtgcccagat agcgatccag
    56761 ggtcaacaac gcgacggtaa ccgtcagaat tgggaaggaa gcaataatca gtacgttcgc
    56821 gcacagtgat gcccaggtaa atactggcat cttgaacatg gtcatgcccg gtgcgcgcat
    56881 cttcagaatg gtaacgaaga agttgatacc ggtaagcgtc gtacctatac cggatagctg
    56941 gagactccat atccagtaat caacaccgac tcccggactg tactctattc ccgatagcgg
    57001 tggataggcc agccagccgg tctgcgcaaa ttcgcccacg ccgagagaaa cgttaaccag
    57061 aatcacacca acaacagtaa accagaagct taagttgttg aggaacggga acgcaacgtc
    57121 acgcgcgccg atctgcagcg gaaccaccag gttcatcaga ccgataacga aaggcatcgc
    57181 cacgaagaag atcataatca cgccgtgcgc ggtgaagatc tgatcgtagt ggtgaggtgg
    57241 caggaagccc gcttcgcccg ccgaggcaag agcctgctgg ctacgcatca taatggcgtc
    57301 agcaaaacca cgcagcaaca tcacaatcgc cacgatgata tacatgatac cgaggcgttt
    57361 atggtcgacg gaggtcagcc actctttcca caggtaggtc cacttaccga agtaagtgat
    57421 caggccaacg agcgccagac ctcccaaaat aatgccagcg atcgtaacca tgacgatagg
    57481 ttcatggaac gggactgcat caagtgataa ttttccgaac atctttattc ttcctcaacc
    57541 cctttaatgg gcggattccg cgtggctcat gtccatgcct tccatacctt cgtgtgcgct
    57601 gtgctcacct tctggctggg tcatgtccat gctcttaccg tgagccataa acttgttaat
    57661 cacatcagca aacaagtctg gtttcacgtt ggagaaatat tccacctggt tgtattcgct
    57721 aggcgcggcc agtttttcga acgcagccat gtcagacatg gtgttcggcg actgcttcgc
    57781 ttttgcgacc cactggtcga attcggcgcg atccggtgtt gcaatagctt tgaacttcat
    57841 gcctgagaag cccgggccgc tgtagctggc ggagataccg tcataagtac cgggttcgtt
    57901 ggcgatcaga tgcaggcgag tctgcatacc agccatggca taaatctggc tacccagacg
    57961 cggaatgaag aaggagttca tcacggagtt ggaggtcact ttgaagtaca ccggagtgtt
    58021 cgccgggaaa gcgatttcat tcacggtagc aatgccctgt tccgggtaga tgaagaacca
    58081 tttccagtcc atggaaacca cttcgatggt aatgggcttc tcgtcgtgtg ccagcggctt
    58141 gctaggctca agagcgtgag tggttttcca ggtcagtacc gcaaggaaga tgatgattaa
    58201 gataggtacc gtccagacca cagcttccac tttattggag tgtgaccagt tcgggctgta
    58261 cttagcatct ttattgctcg cacgatactt ccaggcgaaa ccaacagcca tcaagattgc
    58321 gggaataacg acaatcaaca tcaggccaaa tgccgtcagt atcagtgaac gttgctccag
    58381 accaatctgt cctttgggat ctaacagcgc agaattacag ccactgagca atacagtgcc
    58441 tgcaaataat gacaaccatc ccaaactttt attgtatttc ctgagtctca tttaacgacc
    58501 tcaattccac gggatctggt ggcgtttaaa gtgtgtgggc attttatggg aaggttacat
    58561 tactgtaaac atgattaaat tcctgttact tggatttgac tgtcaggtca catatgttgc
    58621 aaaacatatc aagttgattt taagaagctg ttgcaaaagg gcgttataac gaaaggggag
    58681 tgcagaagga gtttggacgg gcaattggta taaccaatct ggaataaaat aattgtttaa
    58741 caaataatta tcattatggt gacaattggg tggggggaaa ataaagactc gttagctgaa
    58801 tcgtgtaaga aaaaatagaa ttcataaatt gcgtcaataa tttccaaacg ctaagccgca
    58861 caaaagaaca aatattatta agcgatactg atgtttattt agccgtaata attacggcga
    58921 gtgattacta cagctaaata atatttacag attacgtcag atgcgttttt cgtagagcca
    58981 gataatccag caaaccacca agcacgacgc cagaaagggc gactaaaacc ccgacttcca
    59041 gcagagcagg caggaaagag aagtgcgtca aatccagcgc gtccatcgtc aacagcagta
    59101 accacacggc caacaggctg acgcccgccg ccagtgtcca catggcaaag gcataacctg
    59161 ccggatattc ggtacgggag ataaagttgt catttactcg tgtatattca agcgtctggc
    59221 ggcaaaccag cagcaaaata agccccggta cggcagcggc gacggagaat agatagaatg
    59281 tcgaccagcc gtgtgcttca acaaaccaac ccgccacggg gccgacataa actcgtccta
    59341 cagcggaaag cgctgagagc agggcaaatt gagtagcgga aaatgactta ttacacagcg
    59401 tcattaacag cgcgacaaag gctgatgtgc ccatcccgcc acagaggttt tcgaaaaaga
    59461 cggctgcgcc catgctgtag agatgcttat cagtaatcga cagcagccag taaccagcgt
    59521 tagacgcacc ttgtaaaatg ccgaaaatca gcagcgcccg gaacagcgac aggcgctgca
    59581 tcaaaatccc accgtacaat gcgcccacaa tggtcgccag taagccaagc gttttgttaa
    59641 ccacgcctac ttcacccgca tcaaacccga cgccgcgaat caaaaacgtg gttgtcaggc
    59701 tcatggcgaa tgcgtcgccc agcttataca gcacgataag aagcaaaata agccaggcat
    59761 tattgcgacc aaagaaatct cgcagaggtg caacaaccgc ttgttccagc gtttttggca
    59821 caggaatggt gtcggtcggt tctggtgcaa gcaacgtcgc gataatacaa gggatcaaca
    59881 gcgccgccat caaccagtac atgccctgcc agcccagcca tttatctgcc agccacaggg
    59941 ctaagccgcc ggaaaccagc atccctaaac ggtaacccag cacgctgatt gccgcgcccg
    60001 caccacgttc ttctgccggg agcacatcgg ttttccacgc atcgaagaca atatcctggg
    60061 aggccgagca aaaagcgatc accaccgcca gtgccgccat ccagcggagt tgggtgccag
    60121 gttcgagaaa acccatcgcc gcaatggcga ctaataacag gatttgcgtg gcgagcagcc
    60181 aaccgcgccg ccgcccaaaa aatgggggcg tgtagcggtc catcagcggt gaccagagga
    60241 atttaaaaac gtaagcctgg cctaccagag agaagaaacc aatggttttg agatcaatat
    60301 tctcgaccgt catccaggcc tgtaaggtac cggatgtcag ggcgagcggt agcccggaag
    60361 caaaacccag gatcagcaat atggctgaac gcggctgttg aaaaatacgt aaatattgac
    60421 tggacatggg cgtctacagg cccggtttgc accgggccag agggcagaaa ttaacgcgcg
    60481 ttctgcttga tgaattcgtg aatgctggtg tcctgagaca tatcagcgat ggtatcggtc
    60541 agcacactgt taaccgcatc ggcgatattt ttgttggagg cctggaacgc accttcaacg
    60601 ttgtagctgg cacgatagtt tttggttatt ttgttgccat tctgcgcggt agcgatgatg
    60661 gcgatatccg ctttggtagc gatgttgtag cgcacgttgc cctgggacac gtcagcatac
    60721 agttggctaa cgatgatttg cagattaacc gggccattcg gaccaaccat gtaaccacgc
    60781 gcggtcatct gtttttccag cacttcttgc agcaggaaac gcagatcgcg ggaggcggcc
    60841 agggtaacga tttgattatc gcgggtgact tttgccagcg cctgatcggt acgctgatcg
    60901 gcaccattaa tgcttacggt gacgcccatc aggcttggat cctgctgtgg cagtgtaatc
    60961 gtcggggaaa cttcaatagt tgttggcggt tttgcgcatc ctgcaagcat aaacagagca
    61021 actaacggga agaggatttt tttgaacatg ttcgggctct cagagactct taagcgtgtt
    61081 tggtaaaaat tcccgccatc ataacattgc caacggcgag gggaagtggg taaggcatgt
    61141 aaattcatca tgttgacgaa ataatcaccc ctggtaaaag aaacgctgat gcgaggcctg
    61201 tgtttcaatc tttaaatcag taaacttcat acgcttgacg gaaaaaccag gacgaaacct
    61261 aaatatttgt tgttaagctg caatggaaac ggtaaaagcg gctagtattt aaagggatgg
    61321 atgacatctc agcgttgtcg gaggagatat ttcatgatga tacgtgagcg gatagaagaa
    61381 aaattaaggg cggcgttcca acccgtattc ctcgaagtag tggatgaaag ctatcgtcac
    61441 aatgtcccag ccggctctga aagccatttt aaagttgtgc tggtcagcga tcgttttacg
    61501 ggtgaacgtt ttctgaatcg tcatcgaatg atttacagta ctttagcgga ggaactctct
    61561 actaccgttc atgcgctggc tctgcatact tacactatta aggagtggga agggttgcag
    61621 gacaccgtct ttgcctctcc tccctgtcgt ggagcaggaa gcatcgcgta aaaacgcatt
    61681 tgcaactgtc ggcgcttttt cagtatgttg ctaaagattt tatgaaaaac ggcctgcggg
    61741 ccgttttgtt ttgtctggat tttgcgcttt ttgcccagca ttcagacgaa aattgcccgg
    61801 gaattgtgaa aaaatacgcg acagcgcgca ataaccgttc tcgactcata aaagtgatgc
    61861 cgctataatg ccgcgtccta tttgaatgct ttcgggatga ttctggtaac agggaatgtg
    61921 attgattata agaacatccc ggttccgcga agccaacaac ctgtgcttgc ggggtaagag
    61981 ttgaccgagc actgtgattt tttgaggtaa caagatgcaa gtttcagttg aaaccactca
    62041 aggccttggc cgccgtgtaa cgattactat cgctgctgac agcatcgaga ccgctgttaa
    62101 aagcgagctg gtcaacgttg cgaaaaaagt acgtattgac ggcttccgca aaggcaaagt
    62161 gccaatgaat atcgttgctc agcgttatgg cgcgtctgta cgccaggacg ttctgggtga
    62221 cctgatgagc cgtaacttca ttgacgccat cattaaagaa aaaatcaatc cggctggcgc
    62281 accgacttat gttccgggcg aatacaagct gggtgaagac ttcacttact ctgtagagtt
    62341 tgaagtttat ccggaagttg aactgcaggg tctggaagcg atcgaagttg aaaaaccgat
    62401 cgttgaagtg accgacgctg acgttgacgg catgctggat actctgcgta aacagcaggc
    62461 gacctggaaa gaaaaagaca gcgctgttga agcagaagac cgcgtaacca tcgacttcac
    62521 cggttctgta gacagcgaag agttcgaagg cggtaaagcg tctgatttcg tactggcgat
    62581 gggccagggt cgtatgatcc cgggctttga agacggtatc aaaggccaca aagctggcga
    62641 agagttcacc atcgacgtga ccttcccgga agaataccac gcagaaaacc tgaaaggtaa
    62701 agcagcgaaa ttcgctatca acctgaagaa agttgaagag cgtgaactgc cggaactgac
    62761 tgcagaattc atcaaacgtt tcggcgttga agatggttcc gtagaaggtc tgcgcgctga
    62821 agtgcgtaaa aacatggagc gcgagctgaa gagcgccatc cgtaaccgcg ttaagtctca
    62881 ggcgatcgaa ggtctggtaa aagctaacga catcgacgta ccggctgcgc tgatcgacag
    62941 cgaaatcgac gttctgcgtc gccaggctgc acagcgtttc ggtggcaacg aaaaacaagc
    63001 tctggaactg ccgcgcgaac tgttcgaaga acaggctaaa cgccgcgtag ttgttggcct
    63061 gctgctgggc gaagttatcc gcaccaacga gctgaaagct gacgaagagc gcgtgaaagg
    63121 cctgatcgaa gagatggctt ctgcgtacga agatccgaaa gaagttatcg agttctacag
    63181 caaaaacaaa gaactgatgg acaacatgcg caatgttgct ctggaagaac aggctgttga
    63241 agctgtactg gcgaaagcga aagtgactga aaaagaaacc actttcaacg agctgatgaa
    63301 ccagcaggcg taatttacgc agcataacgc gctaaattcg cacaaaggcc cgtcaccgcc
    63361 aggtggtggg ctttttttgt catgaatttt gcatggaacc gtgcgaaaag cctctttcgg
    63421 tgttagcgta acaacaaaag attgttatgc ttgaaatatg gtgatgccgt acccataaca
    63481 cagggactag ctgataatcc gtccataagg ttacaatcgg tacagcaggt tttttcaatt
    63541 ttatccagga gacggaaatg tcatacagcg gcgaacgaga taactttgca ccccatatgg
    63601 cgctggtgcc gatggtcatt gaacagacct cacgcggtga gcgctctttt gatatctatt
    63661 ctcgtctact taaggaacgc gtcatttttc tgactggcca ggttgaagac cacatggcta
    63721 acctgattgt ggcgcagatg ctgttcctgg aagcggaaaa cccagaaaaa gatatctatc
    63781 tgtacattaa ctccccaggc ggggtgatca ctgccgggat gtctatctat gacaccatgc
    63841 agtttatcaa gcctgatgtc agcaccatct gtatgggcca ggcggcctcg atgggcgctt
    63901 tcttgctgac cgcaggggca aaaggtaaac gtttttgcct gccgaattcg cgcgtgatga
    63961 ttcaccaacc gttgggcggc taccagggcc aggcgaccga tatcgaaatt catgcccgtg
    64021 aaattctgaa agttaaaggg cgcatgaatg aacttatggc gcttcatacg ggtcaatcat
    64081 tagaacagat tgaacgtgat accgagcgcg atcgcttcct ttccgcccct gaagcggtgg
    64141 aatacggtct ggtcgattcg attctgaccc atcgtaattg atgccagagg cgcaactgtg
    64201 ccgctatact tatccagggc ggcacaacgc tgtaagcggc ttgcgcctga gaatggcatt
    64261 tgcgtcgtcg tgtgcggcac aaagaacaaa gaagaggttt tgacccatga cagataaacg
    64321 caaagatggc tcaggcaaat tgctgtattg ctctttttgc ggcaaaagcc agcatgaagt
    64381 gcgcaagctg attgccggtc catccgtgta tatctgcgac gaatgtgttg atttatgtaa
    64441 cgacatcatt cgcgaagaga ttaaagaagt tgcaccgcat cgtgaacgca gtgcgctacc
    64501 gacgccgcat gaaattcgca accacctgga cgattacgtt atcggccagg aacaggcgaa
    64561 aaaagtgctg gcggtcgcgg tatacaacca ttacaaacgt ctgcgcaacg gcgataccag
    64621 caatggtgtc gagttgggca aaagtaacat tctgctgatc ggtccgaccg gttccggtaa
    64681 aacgctgctg gctgaaacgc tggcgcgcct gctggatgtt ccgttcacca tggccgacgc
    64741 gactacactg accgaagccg gttatgtggg tgaagacgtt gaaaacatca ttcagaagct
    64801 gttgcagaaa tgcgactacg atgtccagaa agcacagcgt ggtattgtct acatcgatga
    64861 aatcgacaag atttctcgta agtcggacaa cccgtctatt acccgtgacg tttccggtga
    64921 aggcgtacag caggcactgt tgaaactgat cgaaggtact gttgctgccg ttccaccgca
    64981 gggcgggcgt aagcatccgc agcaggaatt tttgcaggtt gatacctcca agatcctgtt
    65041 tatttgtggc ggtgcgtttg ccggtctgga taaagtgatt tcccaccgtg tagaaaccgg
    65101 ctccggcatt ggttttggcg cgacggtaaa agcgaagtcc gacaaagcaa gcgaaggcga
    65161 gctgctggcg caggttgaac cggaagatct gatcaagttt ggtcttatcc ctgagtttat
    65221 tggtcgtctg ccggttgtcg caacgttgaa tgaactgagc gaagaagctc tgattcagat
    65281 cctcaaagag ccgaaaaacg ccctgaccaa gcagtatcag gcgctgttta atctggaagg
    65341 cgtggatctg gaattccgtg acgaggcgct ggatgctatc gctaagaaag cgatggcgcg
    65401 taaaaccggt gcccgtggcc tgcgttccat cgtagaagcc gcactgctcg ataccatgta
    65461 cgatctgccg tccatggaag atgtcgaaaa agtggttatc gacgagtcgg taattgatgg
    65521 tcaaagcaaa ccgttgctga tttatggtaa gccggaagcg caacaggcat ctggtgaata
    65581 attaaccatt cccatacaat tagttaacca aaaagggggg attttatctc ccctttaatt
    65641 tttcctctat tctcggcgtt gaatgtgggg gaaacatccc catatactga cgtacatgtt
    65701 aatagatggc gtgaagcaca gtcgtgtcat ctgattacct ggcggaaatt aaactaagag
    65761 agagctctat gaatcctgag cgttctgaac gcattgaaat ccccgtattg ccgctgcgcg
    65821 atgtggtggt ttatccgcac atggtcattc ccttatttgt cgggcgggaa aaatctatcc
    65881 gttgtctgga agcggcgatg gaccatgata aaaaaattat gctggtcgcg cagaaagaag
    65941 cttcaacgga tgagccgggt gtaaacgatc ttttcaccgt cgggaccgtg gcctctatat
    66001 tgcagatgct gaaactgcct gacggcaccg tcaaagtgct ggtcgagggg ttacagcgcg
    66061 cgcgtatttc tgcgctctct gacaatggcg aacacttttc tgcgaaggcg gagtatctgg
    66121 agtcgccgac cattgatgag cgggaacagg aagtgctggt gcgtactgca atcagccagt
    66181 tcgaaggcta catcaagctg aacaaaaaaa tcccaccaga agtgctgacg tcgctgaata
    66241 gcatcgacga tccggcgcgt ctggcggata ccattgctgc acatatgcca ctgaaactgg
    66301 ctgacaaaca gtccgttctg gagatgtccg acgttaacga acgtctggaa tatctgatgg
    66361 caatgatgga atcggaaatc gatctgctgc aggttgagaa acgcattcgc aaccgcgtta
    66421 aaaagcagat ggagaaatcc cagcgtgagt actatctgaa cgagcaaatg aaagctattc
    66481 agaaagaact cggtgaaatg gacgacgcgc cggacgaaaa cgaagccctg aagcgcaaaa
    66541 tcgacgcggc gaagatgccg aaagaggcaa aagagaaagc ggaagcagag ttgcagaagc
    66601 tgaaaatgat gtctccgatg tcggcagaag cgaccgtagt gcgtggttat atcgactgga
    66661 tggtacaggt accgtggaat gcgcgcagca aggtcaaaaa agacctgcgt caggcgcagg
    66721 aaatccttga taccgaccat tatggtctgg agcgcgtgaa agatcgcatc cttgagtacc
    66781 ttgcggttca aagccgtgtc aacaaaatca agggaccgat cctctgcctg gtagggccgc
    66841 cgggggtagg taaaacctct cttggtcagt ccattgccaa agccaccggg cgtaaatatg
    66901 tccgtatggc gctgggcggc gtgcgtgatg aagcggaaat ccgtggtcac cgccgtactt
    66961 acatcggttc tatgccgggt aaactgatcc agaaaatggc gaaagtgggc gtgaaaaacc
    67021 cgctgttcct gctcgatgag atcgacaaaa tgtcttctga catgcgaggc gatccggcct
    67081 ctgcactgct tgaagtgctg gatccagagc agaacgtagc gttcagcgac cactacctgg
    67141 aagtggatta cgatctcagc gacgtgatgt ttgtcgcgac gtcgaactcc atgaacattc
    67201 cggcaccgct gctggatcgt atggaagtga ttcgcctctc cggttatacc gaagatgaaa
    67261 aactgaacat cgccaaacgt cacctgctgc cgaagcagat tgaacgtaat gcactgaaaa
    67321 aaggtgagct gaccgtcgac gatagcgcca ttatcggcat tattcgttac tacacccgtg
    67381 aggcgggcgt gcgtggtctg gagcgtgaaa tctccaaact gtgccgcaaa gcggttaagc
    67441 agttactgct cgataagtca ttaaaacata tcgaaattaa cggcgataac ctgcatgact
    67501 acctcggtgt tcagcgtttc gactatggtc gcgctgataa cgaaaactgt gtcggtcagg
    67561 taaccggtct ggcgtggacg gaagtgggcg gtgacttgct gaccattgaa accgcatgtg
    67621 ttccgggtaa aggcaaactg acctataccg gttcgctcgg cgaagtgatg caggagtcta
    67681 ttcaggcggc gttaacggtg gttcgtgcgc gtgcggaaaa actggggatc aaccctgatt
    67741 tttacgaaaa acgtgacatc cacgtccacg taccggaagg tgcgacgccg aaagatggtc
    67801 cgagtgccgg tattgctatg tgcaccgcgc tggtttcttg cctgaccggt aacccggttc
    67861 gtgccgatgt ggcaatgacc ggtgagatca ctctgcgtgg tcaggtactg ccgatcggtg
    67921 gtttgaaaga aaaactcctg gcagcgcatc gcggcgggat taaaacagtg ctaattccgt
    67981 tcgaaaataa acgcgatctg gaagagattc ctgacaacgt aattgccgat ctggacattc
    68041 atcctgtgaa gcgcattgag gaagttctga ctctggcgct gcaaaatgaa ccgtctggca
    68101 tgcaggttgt gactgcaaaa tagtgacctc gcgcaaaatg cactaataaa aacagggctg
    68161 gcaggctaat tcgggcttgc cagccttttt ttgtctcgct aagttagatg gcggatcggg
    68221 cttgccctta ttaaggggtg ttgtaagggg atggctggcc tgatataact gctgcgcgtt
    68281 cgtaccttga aggattcaag tgcgatataa attataaaga ggaagagaag agtgaataaa
    68341 tctcaattga tcgacaagat tgctgcaggg gctgatatct ctaaagctgc ggctggccgt
    68401 gcgttagatg ctattattgc ttccgtaact gaatctctga aagaagggga tgatgtagca
    68461 ctggtaggtt ttggtacttt tgccgttaaa gagcgtgctg cccgtactgg ccgcaacccg
    68521 cagaccggta aagagatcac catcgctgct gctaaagtac cgagcttccg tgcaggtaaa
    68581 gcactgaaag acgcggtaaa ctaagcgttg tccccggtgg ggatgtgacg aagttcaagg
    68641 gcgcatctac tgatgtgcct tttttatttg tattcggtga ctttctgcgt cttgtgggct
    68701 gacaattgcc cccgtttctt gtcacaatag gcctttgcgc gcatcgatac gttgcgtgag
    68761 gtacacagtc atctacagcg gagtgttgtt acaccatgat ggacagctta cgcacggctg
    68821 caaacagtct cgtgctcaag attattttcg gtatcattat cgtgtcgttc atattgaccg
    68881 gcgtgagtgg ttacctgatt ggcggaggca ataactacgc cgcaaaagtg aatgaccagg
    68941 aaatcagccg tgggcagttc gagaacgcct ttaacagcga gcgtaatcgc atgcagcaac
    69001 agctgggcga tcaatactcc gagctggcag cgaatgaagg ctatatgaaa accctgcgtc
    69061 aacaggtgct gaatcgtctg atcgacgagg cgctgttgga tcagtacgct cgtgagctga
    69121 aactgggtat cagtgatgag caggttaaac aggcgatttt cgcgacccca gccttccagg
    69181 ttgacggcaa atttgataac agccgctata acggtatcct caaccagatg gggatgaccg
    69241 ccgatcagta cgcccaggcg ctgcgtaacc agctcactac ccaacagctg attaacggtg
    69301 ttgccggtac cgattttatg ctgaaaggtg aaaccgacga gctggcggca ctggtcgcgc
    69361 aacaacgcgt ggtgcgtgag gcgactatcg atgttaacgc gctggcggcg aagcagcctg
    69421 tgaccgaaca ggagattgcc agctactacg aacaaaacaa aaacaatttc atgacgccgg
    69481 aacaattccg cgtgagttac atcaagctgg atgccgcaac gatgcagcaa ccggttagcg
    69541 atgcggatat ccagagctac tacgatcagc atcaggatca attcacccag ccgcagcgta
    69601 cccgctacag catcatccag accaaaactg aagatgaagc gaaagcggta cttgatgagc
    69661 tgaataaagg cggtgatttt gctgcattag ccaaagaaaa atctgccgat attatctctg
    69721 cccgtaacgg cggcgatatg ggttggttag aagatgccac tatccccgac gaactgaaaa
    69781 atgctggtct gaaagaaaaa ggccaactgt ctggtgtcat caaatcttcg gtcggtttcc
    69841 tgattgtacg tctggacgac attcagccag cgaaagtgaa atcgttagac gaagtacgtg
    69901 acgacattgc ggcgaaagtg aaacacgaaa aagccctcga tgcgtactat gcgctgcagc
    69961 agaaagtgag cgatgcggca agcaacgaca ccgagtctct ggccggtgca gagcaagctg
    70021 ccggcgttaa agccactcag acgggttggt tcagcaaaga taacctgccg gaagagttga
    70081 acttcaagcc ggttgccgac gctatcttta acggcggtct ggtaggtgaa aacggcgcgc
    70141 cgggcatcaa ctctgacatc atcaccgtag acggcgaccg tgcattcgtg ctgcgcatca
    70201 gcgagcacaa accggaagcg gtgaaaccgt tggcagatgt tcaggaacaa gttaaggcac
    70261 tggttcagca caacaaagct gaacaacagg cgaaagtgga tgctgagaaa ctgctggttg
    70321 atttgaaagc cggcaaaggt gcggaagcta tgcaggctgc cggtctgaaa tttggcgagc
    70381 cgaaaacctt aagccgttcc ggtcgtgacc cgattagcca ggcggcgttt gcacagccac
    70441 tgccagcgaa agacaaaccg agctacggta tggcgaccga tatgcaaggt aatgtggttc
    70501 tgctggcgct ggatgaagtg aaacaaggtt caatgccgga agatcagaaa aaagcgatgg
    70561 tgcagggtat cacccagaac aacgcacaaa tcgtctttga agctctgatg agtaacctgc
    70621 gtaaagaggc gaaaatcaaa attggcgatg cgctggaaca gcaataatcc tgaagccgcc
    70681 tcgcaaaaaa atgcgttgca gctgtaacaa gaaaaggtcg ctttcgcggc cttttccatt
    70741 tctgaacatt gccatttgtt tactgttttc actgccgtta aggtgattcc actgttaaca
    70801 aacaaggaga aaacagtatg aaacacggaa ttaaagcact gctcattacc ctgtccctgg
    70861 cctgtgccgg aatgtctcat agcgcgctgg cggcagcttc tgtggcgaaa ccgacggcgg
    70921 tagaaaccaa agcggaagct cctgcagcac aaagtaaagc agcagtaccg gcgaaagcca
    70981 gtgacgaaga aggcacccgg gtcagcatta ataatgccag cgcggaagag ctagcccgcg
    71041 cgatgaatgg cgttggcctg aagaaagcgc aggcgattgt cagttatcgc gaagagtacg
    71101 gtccgtttaa aacagtggag gatctaaagc aggtgccggg gatgggcaat tcgctggtgg
    71161 aacgtaatct ggcggtatta accctgtaat taatttgcat agtggcaatt ttttgccaga
    71221 ctgaagaggt cataccagtt atgacctctg tacttataac aacaacgtaa ggttattgcg
    71281 ctatgcaaac acaaataaaa gttcgtggat atcatctcga cgtttaccag cacgtcaaca
    71341 acgcccgcta ccttgaattt ctcgaagaag cccgctggga tgggttggaa aatagcgaca
    71401 gttttcagtg gatgacggcc cataacatcg ccttcgtcgt ggtcaatatc aatattaact
    71461 atcgtcgccc agcggtatta agtgacctgt taactattac cagtcagttg cagcaattaa
    71521 acggtaaaag cggcatctta agccaggtca ttacactgga gccggaaggg caggtggtag
    71581 cggatgcgct tattacgttt gtttgtattg atcttaaaac gcagaaagca ttagctctgg
    71641 aaggggaatt gcgcgaaaag ctggagcaga tggttaagta aacgttttgt ggtgccggat
    71701 gctcaagccg catccggcga cacccggaat aattacctca acccggtttt ctgcttcatc
    71761 gctgccatca ccgtcggttt atcggccaga taatgattca aaccgttggc gcgtaaatta
    71821 catgccgcac aatgaccgca accgtcgcct ttaatgccgt tatagcaggt caacgtttcg
    71881 ttacggacta aatccagttt gccgtaataa tctgccagcg cccaggtttc cgctttatca
    71941 atccacatca gcggcgtttc aaaacgaata tctttcgcca tgcccaaact gacggcatgg
    72001 tttagtgctt tcacaaactc atcgcggcaa tccgggtagc cggagaaatc cgtttcacag
    72061 acgccggtaa ttacggcttc tgcttttacc tgatacgcat atattgccgc cagcgtcagg
    72121 aacaaaatat tacgccctgg gacaaacgta ttcgggatgc catcggcttc aggttcataa
    72181 tcaggcaccg gaatgctgtc acgtgtcagg ctgctgaccg ccagctcgtt gagcagcgtt
    72241 acatcaagaa ccttatgcgc gcgtgctccc agtttcagcg ccagttcgcg tgccacgtcg
    72301 atttctgcgc gatgtcgctg accgtaatcg aacgtcacgc aatggacttc atcatattgt
    72361 tgtaatgcct gcaccagaca ggtggtggaa tcctggcctc cactgaacac aacgacagca
    72421 cgtttcatag ataatcctgc ttgaacaata aaagcgttat ggtaacgcct gcgattaacc
    72481 cggaccagct attcattgcg acggcgcggg aagccaggct tcggtaaatt caaaccagcc
    72541 gcgcggtgtc agtcgcacac cgttcacgcc gggaggggca ctaatgcgat agtgatagtt
    72601 gaacagcggc gtcagcgtcg cgtctgccat taactggcta aaaaccgctt tcagggcatt
    72661 aaatcggttt tcctcatcag gcattacttg caccgcgtcc agcgtcgatt gcagatgtgc
    72721 atatgctgga gcgtcgaaaa catgtggcca tagtggatcg caacgtagcc attgctccag
    72781 agtatattcc ggtgcttcgc caattaatct gtcgcccatc atgaggtctg cgtgtgccag
    72841 tagggtcgtg tcgtcccagt tttttgcgtt atgaaaaata attgtgagtt cacagccttc
    72901 cgctgccagt gtcgcctgta ggcgttctgc catggtatga agttctatcg gtaggtgata
    72961 aaccagcgtc aaggttttcg gtagtttgac ttcatccggt acttgccagt gcggaatagt
    73021 ccagcctggc agtaatgcat gactggcggt gatcaggttt tctccgactt ctaacgtttg
    73081 taataaaccg gattgatgaa taatggagat cacttttcgc gcctgccaga gggagagtcg
    73141 gggacttttg cgcaacgtta aatagcaaaa acctaaactg atgccactac tgacctggct
    73201 gacccgttgc agctcctccg gtttgccgat ggtgatttgc acgggatgcc gacaactggt
    73261 tcccaaatct ttttcgaaaa gcggcggagt tatccagtac tcaaccgctt taagcagcgg
    73321 atgacgcaaa tggtaataat catggctctc caggcgcacc agctccgctg tgaattgtgt
    73381 taagcgaaaa ggaccagtgc cgatcagtgg gaattgcgga tgcgccagat ggctgcaata
    73441 gctcgccagc cggtgcgcaa gccagtaatc ggggcgatgt aaaaagaagg tcagacactg
    73501 cggatgggtg acttcaatac gcttcacgct aataaataat tgatccagtg ctggcagttg
    73561 taacagcatc aataatcgct ggtgtaagtg tgaggctttt actgcatcgc cgttatgcca
    73621 gtgtagggtt gaacgaagat aaaagtccca gcgtaacccg tcagtagagg tttcccagtg
    73681 atgcgctaaa tcgccaatcg ggcgctgagt attattatcg aagcgggtca ggccggaaaa
    73741 tatctgcccg gcgagatgct gctcggcacg gccgggcaaa aagcctggtt gtagcggttc
    73801 gagcgggcga tagtagggaa tacgcaacgt gggtgtatcg ttttgccatt gtccgcccat
    73861 aaacggctgt aacagagtgc gtagctcacc tggggccagt tgcgccagct ccagcacatc
    73921 ttgctgcttt ccggtttcca gtgcctgttc catcatcgca ttgcgtagcg attccggcgt
    73981 gaccagaaag cgtaattgtc cgcgctttcc gcgtcctgac tgcgcctgcc actccagcca
    74041 tcccgcctcc tgtgcctgac gcaacagcgt acgaacatgg cgttcgctgc aaaaacagcg
    74101 ttcggccagt tcgctgacgg tgacggtttg cggctttccg gctgaaggtt gccacagacg
    74161 ttgatactgg ttaagacggt tgagcaatcg catataaacc cggaacaata ttatttaact
    74221 attcactatt acttccgtat atatcaggtg atactcaatc tccattaacc gtgtcacaga
    74281 gtggagaaag aaatggctcg tctggcagca tttgatatgg atggcacttt attgatgcct
    74341 gaccatcatt taggtgagaa aaccctctct actttggcgc gactgcgtga acgcgacatt
    74401 accctcactt ttgccacggg gcgtcatgcg ctggagatgc agcatattct cggggcgcta
    74461 tcgctggatg catatttgat taccggcaac ggaacgctcg tgcattctct ggaaggtgaa
    74521 cttttacatc gtgatgattt acctgcggat gtcgcggagc tggtgctgta tcagcaatgg
    74581 gatacccgag ccagcatgca tatcttcaat gacgacggtt ggtttaccgg gaaagagatc
    74641 cctgcgttgt tgcaggcatt tgtctatagc ggttttcgtt atcagataat cgatgtcaaa
    74701 aaaatgccac tcggcagcgt caccaagatc tgcttctgtg gcgatcacga cgatcttaca
    74761 cgcttgcaga tccagctata cgaagcatta ggcgagcgtg cacatttgtg tttttccgcc
    74821 acggattgcc tcgaagtgct gccggtgggc tgcaataaag gcgctgcatt gacggtgctg
    74881 acccaacatc taggtttatc gttgcgcgat tgcatggcct ttggtgatgc gatgaacgat
    74941 cgcgaaatgt taggcagcgt cggtagcggg tttattatgg gcaatgcgat gccgcaactg
    75001 cgcgcggagc tcccgcattt accggtgatt ggacattgcc gaaatcaggc tgtctctcac
    75061 tatttgacgc actgactgga ctatccacat ctaccttatt cccccgaata atgagatccc
    75121 ttccagcacc gggcaattgc ccggtttttt ttgcgttgaa tttgtcattt tgtgccgtgg
    75181 tgtttaaacc gcacagaata aattgtcgtg atttcacctt taaaatagaa ttaaaagaga
    75241 aaaaaattct ctgtggaagg gctatgttag ataaaattga ccgtaagctg ctggccttac
    75301 tgcagcagga ttgcaccctc tctttgcagg cactggctga agccgttaat ctgacaacca
    75361 ccccttgctg gaagcgcctg aaacggctgg aggacgacgg tatccttatc ggcaaagtcg
    75421 ccctgctgga tccggaaaaa ataggcctcg gcctgaccgc ctttgtgctg ataaaaacgc
    75481 aacatcacag tagcgaatgg tattgccgct ttgtcacggt ggttaccgaa atgccagaag
    75541 tgctggggtt ctggcgcatg gctggtgaat acgattatct gatgcgcgtc caggttgccg
    75601 acatgaaacg ctacgacgag ttttataagc gtctggtaaa cagcgtgccg gggctgtcgg
    75661 acgtcacttc cagcttcgcg atggaacaga ttaaatacac cacttcttta cccatcgaat
    75721 aaatatccag aatcaggtca ggacacaacg cgtgcgatta tttgctcaat taagctggta
    75781 tttccgtcgg gaatggcgtc gctatctcgg ggctgtcgcc ttgcttgtca ttatcgcgat
    75841 gctgcaactg gttccgccaa aagtggttgg tattgttgtc gatggcgtga cagaacaaca
    75901 ctttactacc gggcagatcc tgatgtggat cgccaccatg gtgctgattg ccgttgtggt
    75961 ttatttcctg cgttacgtct ggcgggtatt gctgtttggt gcgtcttatc aactggctgt
    76021 tgaactgcgt gaagattatt accgtcagct aagccggcag catcctgagt tttacctgcg
    76081 tcatcgcacg ggtgacctca tggctcgtgc gaccaatgac gtcgatcgcg tcgtgtttgc
    76141 agccggagaa ggggtgctga cgctggtgga ttcactggtg atgggctgcg ctgtgttgat
    76201 tatgatgtct acgcaaatta gctggcagtt gaccttattt gccctgttgc cgatgccagt
    76261 gatggcgatc atgatcaagc gcaacggcga tgccttgcat gaacgcttta agctggcaca
    76321 ggcggcgttt tctagtctta atgaccgcac ctaggaaagc ctcaccagta tccgcatgat
    76381 caaagccttt ggtctggaag atcgccagtc ggcgttattt gccgcggatg ccgaagatac
    76441 cggcaaaaaa aacatgcggg tggcgcgtat tgatgctcgt ttcgacccga ccatctatat
    76501 cgcgattggt atggcgaact tgctggcgat tggcggcggt agttggatgg tggtgcaggg
    76561 cagtttaacg ctgggccagc tcaccagttt tatgatgtat ttaggtctga tgatttggcc
    76621 aatgctggcg ctggcatgga tgtttaacat tgtggaacgt ggtagtgctg catacagccg
    76681 tattcgcgcg atgctggcgg aagcgccggt ggtgaacgat ggtagcgaac ccgtgccgga
    76741 agggcgtggc gaactggatg taaatattca ccagttcacg tatccgcaga ctgaccatcc
    76801 tgcgctggaa aacgtcaatt tcgccctgaa acccggtcag atgctgggta tctgcgggcc
    76861 gactggttcc ggcaaaagta ccctgttgtc gctcattcag cgtcatttcg acgtcagcga
    76921 gggggatatt cgctttcatg atattcctct gacgaagtta caactcgata gctggcgtag
    76981 ccgcctggcg gtagttagcc agacaccgtt ccttttttcc gacaccgtag cgaacaacat
    77041 cgcgttgggt tgcccgaatg ccacccagca agagattgag catgtcgcgc ggttagccag
    77101 cgtacatgac gatattttgc gtctaccgca aggttacgat acagaagtgg gcgagcgcgg
    77161 cgtgatgctt tccggtgggc aaaaacagcg tatctccatt gctcgagcgt tattagtcaa
    77221 cgcggaaatc ctcatccttg atgatgcgct ttcggcggtg gacggacgca cagagcacca
    77281 gatcctgcat aacctgcgtc agtggggaca gggaagaacg gtaatcatca gtgcccatcg
    77341 cctttcggca ctgacggaag ccagtgaaaa tgtaatgaac gcatcccaca caattaaaca
    77401 cggagcagta tgatggttaa aagggtgaaa ctggcaggtg tggagcctcg cagatttccg
    77461 gcatcatagt gcccaatacg ggatgcataa agctccctca gactggagac tccacaaatg
    77521 aaatatacac cggttggcgt tgatatcgca aaacatgtca ttcagattca cttcatcaat
    77581 gagcacacag
//
LOCUS       DADLFA010000002        64618 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000002 DADLFA010000000
VERSION     DADLFA010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 64618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64618
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.002"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..94
                     /locus_tag="JQH65_000077"
     CDS             <1..94
                     /locus_tag="JQH65_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY6137607.1"
                     /translation="LNAVLDYIDAVAATDTSTAPDVIWPELPEA"
     gene            complement(66..503)
                     /locus_tag="JQH65_000078"
     CDS             complement(66..503)
                     /locus_tag="JQH65_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY6137608.1"
                     /translation="MFYSPSLNIFVNPALKDDYINANSWPDDALAVSDDVYNEFAINT
                     PPDDKIRVAGKNGLPTWALIPPPSYEELIQQAESERQLLLNQANEYMNSKQWPGKAAI
                     GRLKGEELAQYNLWLDYLDALELIDTSSAPDIEWPTPPAVQAR"
     gene            complement(505..891)
                     /locus_tag="JQH65_000079"
     CDS             complement(505..891)
                     /locus_tag="JQH65_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY6137609.1"
                     /translation="MPVSHYLCIFLNVGLGELSLAGTASGVIGLNGYVTIPLIISGSR
                     RTLIIQWGQARFGGSGGEDAGYLNDFPFAFPSACYGMIVSHVGHTPSGAGILSASAIT
                     SNQFRGFSSIATAANAVLGRYIAIGV"
     gene            922..1488
                     /locus_tag="JQH65_000080"
     CDS             922..1488
                     /locus_tag="JQH65_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016529756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY6137610.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALNCAGCERIFEDKISGTKPDRPG
                     LKKLLRTLSAGDTLVVWKLDRLGRSMRHLVTLIEELRQRGVNFRSLTDSIDTSTPMGR
                     FFFHVMGALAEMERELIVERTRAGLAAARAKGRVGGRRPKLTTEQWAQIGRLLEAGES
                     RQRIALIFDVGVSTIYRKFPANESNESP"
     gene            1631..2803
                     /locus_tag="JQH65_000081"
     CDS             1631..2803
                     /locus_tag="JQH65_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HAY6137611.1"
                     /translation="MAQDYHHGVRVVEVNEGTRSITTVSTAIVGMVCTGDDADAKMFP
                     LNKPVLITDVLTASGKAGESGTLARSLDAIADQAKPVTVVVRVPQGETEEETTTNIIG
                     AVTAEGKKTGMKALLSAQSQLGVKPRILGVPGHDTKAVATELLSVAQSLRGFAYLSAY
                     GCKTVQEAITYRENFSQREGMLIWPDFTGWDTVLNAEATAYATARALGLRAKIDEQTG
                     WHKSLSNVGVNGVTGISADVFWDLQDPATDAGLLNQNDITTLIRKDGFRFWGSRCLSD
                     DPLFAFENYTRTAQVLMDTMAEAHMWAVDKPLNPSLARDIIEGIRAKMRSLVSQGYLI
                     GGDCWLDESVNDKDTLKAGKLTIDYDYTPVPPLENLMLRQRITDQYLVNFSSQVSA"
     gene            2813..3328
                     /locus_tag="JQH65_000082"
     CDS             2813..3328
                     /locus_tag="JQH65_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HAY6137612.1"
                     /translation="MALPRKLKHLNLFNDGNNWQGIVESLTLPKFTRKYEKYRGGGMP
                     GAVDVDLGLDDSALDTEFSIGGTELLLFKQMGKATVDGIQLRFTGSIQRDDTGEVQAV
                     ELVVRGRHKEVDSGEWKTGESNTTKVTSTNSYAKLTINGEVLYEVDLINMVEIVDGVD
                     LMEAHRNALGL"
     gene            3383..3685
                     /locus_tag="JQH65_000083"
     CDS             3383..3685
                     /locus_tag="JQH65_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HAY6137613.1"
                     /translation="MSDKQTEKTIQLDTPIMRGKTEITEIVLRKPQSGALRGTRLQAI
                     MDMDVNAMMTVIPRISSPALTAQEIAEMDPADLTAMSVEVVTFLLKKSVLAGLPTA"
     gene            3700..3819
                     /locus_tag="JQH65_000084"
     CDS             3700..3819
                     /locus_tag="JQH65_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HAY6137614.1"
                     /translation="MADIATIFHWPPSVTDVMPLTEVLEWRDKAIQRSGASHE"
     gene            3812..6889
                     /locus_tag="JQH65_000085"
     CDS             3812..6889
                     /locus_tag="JQH65_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAY6137615.1"
                     /translation="MSDNNLRLQVILNAVDKLTRPFRSAQASSRELAAAVKKSRDAIK
                     QLDQAGSSLDSFRKLQAENQKLGDRLNYARQRANLLSQELGAMGPPSQRQVVALGRQR
                     LAVQRLEERQKKLQQQTALVRAELYRAGISANDGASATARITRETMRYNRQLSEQEAR
                     LRRVGEQQRKMHAARGAYARRLEVRDRIAGAGATTTAAGLAMGAPVMAAVKSYTSMED
                     AMKGVAKQVNGLRDDNGNRTARFYEMQGAIKAASEQLPMENGAVDFAALVEGGARMNV
                     ANPDDSWEDQKRDLLAFASTAAKAATAFELPADELSESLGKIAQLYKIPTRNIEQLGD
                     ALNYLDDNAMSKGADIIDVMQRLGGVADRLDYRKAAALGSTFLTLGAAPEVAASAANA
                     MVRELSIATMQSKSFFEGMNLLKLNPEVIEKQMTKDAMGTIQRVLEKVNALPQDKRLS
                     AMTMLFGKEFGDDAAKLANNLPELQRQLKLTAGNDALGSMQKESDINKDSLSAQWLLV
                     KTGAQNTFSSLGETLRQPLMDILYTVKSVTGALRRWVEANPELTGTLMKVAAVVAAVT
                     VGLGTLAVALAAVLGPLAVIRLGFSVLGIKTLPSVTATVTRTSNALSWLAGAPLALLR
                     RGLASSGNAAGLLTAPLSSLRRTASLTGNVLKTVAGAPVALLRSGLSGLRAVAVMFMN
                     PLAVLRGGLAATGTVLRMLASGPLAMLRVALYAVSGLLGALLSLIGLVVTALAGVALV
                     VWKYWQPITAFLGGVVEGFKAAAGPVSAAFEPLKPVFQWIGDKVQALWGWFTDLLTPV
                     KSTSAELQSAAAMGRRFGEALAEGLNMVMHPLDSLKSGVSWLLEKLGIVSKEAAKAKL
                     PESVTRQQPATVNADGKVMMPSGGFPSWGYGFAGMYDSGGYIPRGQFGIVGENGPEIV
                     NGPANVTSRRNTAALAAVVAGMMGVAATPAELPPLHPLALPAKGGEAMVSRAATVPPV
                     QRIEAPMQIIIQTQPGQSAQDIAREVARQLDERERRLKAKARSNYSDQGGYDA"
     gene            6886..7371
                     /locus_tag="JQH65_000086"
     CDS             6886..7371
                     /locus_tag="JQH65_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY6137616.1"
                     /translation="MMMVLGLYVFMLRTVPYQELQYQRSWRHAANSRVNRRPSTQFLG
                     PDNDMLTLSGVLMPEVTGGRLSLLALEQMAEQGKAWPLIEGSGTIYGMYVIEGLNQTK
                     TEFFRDGMPRRIEFTLSLKRVDESLSDMFGDLSAQLNNLQGTATSALSDISKTVGGLL
                     S"
     gene            7368..8468
                     /locus_tag="JQH65_000087"
     CDS             7368..8468
                     /locus_tag="JQH65_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HAY6137617.1"
                     /translation="MNFSSELLNKGNKTPAFSISIEGRDITTVLDNRLMGLTLTDNRG
                     FEADQLDLELDDADGKIVLPRRGAVITLALGWKGQPLFPKGAFTVDEIEHTGAPDRLT
                     IRARSADFRETLNTRREKSWHKTTVGEVVKEIAARHKLKMALGEDLSDKLVEHIDQTN
                     ESDGSFLMRLARQYGAIASVKNGNLLFIRQGQGKSASGKPLPVITITRKDGDSHRFTL
                     ADRGAYTGVIASWLHTREPAKKESTTVKRKRRTKKQKKEPEAKQGDYLVGTDENVLVL
                     NRTYANRSNAERAAKMQWERLQRGVASFSLQLAEGRADLYTEMPVKVSGFKQPLDDAE
                     WTITTLTHTVSPDNGFTTSLELEVRIDDFEME"
     gene            8559..8777
                     /locus_tag="JQH65_000088"
     CDS             8559..8777
                     /locus_tag="JQH65_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001504085.1"
                     /note="in E. coli K-12 this is in prophage P2 remnant;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY6137618.1"
                     /translation="MMICPLCGSAAHTRSSFQVSSLTKERYNQCQNINCSHTFVTHET
                     FVRSIATPKESNPVQPHPMKSGQVALSL"
     gene            complement(9013..10698)
                     /locus_tag="JQH65_000089"
     CDS             complement(9013..10698)
                     /locus_tag="JQH65_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HAY6137619.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTAHSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            10968..11345
                     /locus_tag="JQH65_000090"
     CDS             10968..11345
                     /locus_tag="JQH65_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6137620.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(11375..11632)
                     /gene="grxA"
                     /locus_tag="JQH65_000091"
     CDS             complement(11375..11632)
                     /gene="grxA"
                     /locus_tag="JQH65_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HAY6137621.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            11792..12079
                     /locus_tag="JQH65_000092"
     CDS             11792..12079
                     /locus_tag="JQH65_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HAY6137622.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            12063..12785
                     /gene="nfsA"
                     /locus_tag="JQH65_000093"
     CDS             12063..12785
                     /gene="nfsA"
                     /locus_tag="JQH65_000093"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HAY6137623.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            12846..13748
                     /gene="rimK"
                     /locus_tag="JQH65_000094"
     CDS             12846..13748
                     /gene="rimK"
                     /locus_tag="JQH65_000094"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HAY6137624.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIVHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERYATTEYCLKTGG"
     gene            13836..14312
                     /locus_tag="JQH65_000095"
     CDS             13836..14312
                     /locus_tag="JQH65_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HAY6137625.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            14663..15775
                     /gene="potF"
                     /locus_tag="JQH65_000096"
     CDS             14663..15775
                     /gene="potF"
                     /locus_tag="JQH65_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HAY6137626.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            15870..17003
                     /gene="potG"
                     /locus_tag="JQH65_000097"
     CDS             15870..17003
                     /gene="potG"
                     /locus_tag="JQH65_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HAY6137627.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTQLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVHEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERHEDGLVLYSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            17013..17966
                     /gene="potH"
                     /locus_tag="JQH65_000098"
     CDS             17013..17966
                     /gene="potH"
                     /locus_tag="JQH65_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HAY6137628.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVAAAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            17963..18808
                     /gene="potI"
                     /locus_tag="JQH65_000099"
     CDS             17963..18808
                     /gene="potI"
                     /locus_tag="JQH65_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HAY6137629.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            18868..19356
                     /locus_tag="JQH65_000100"
     CDS             18868..19356
                     /locus_tag="JQH65_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HAY6137630.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            19397..20524
                     /gene="rlmC"
                     /locus_tag="JQH65_000101"
     CDS             19397..20524
                     /gene="rlmC"
                     /locus_tag="JQH65_000101"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HAY6137631.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGFRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(20699..21430)
                     /gene="artJ"
                     /locus_tag="JQH65_000102"
     CDS             complement(20699..21430)
                     /gene="artJ"
                     /locus_tag="JQH65_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY6137632.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(21721..22389)
                     /gene="artM"
                     /locus_tag="JQH65_000103"
     CDS             complement(21721..22389)
                     /gene="artM"
                     /locus_tag="JQH65_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HAY6137633.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(22389..23105)
                     /gene="artQ"
                     /locus_tag="JQH65_000104"
     CDS             complement(22389..23105)
                     /gene="artQ"
                     /locus_tag="JQH65_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HAY6137634.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESEKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(23112..23843)
                     /gene="artJ"
                     /locus_tag="JQH65_000105"
     CDS             complement(23112..23843)
                     /gene="artJ"
                     /locus_tag="JQH65_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY6137635.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKTVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(23861..24589)
                     /gene="artP"
                     /locus_tag="JQH65_000106"
     CDS             complement(23861..24589)
                     /gene="artP"
                     /locus_tag="JQH65_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HAY6137636.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(24807..25322)
                     /locus_tag="JQH65_000107"
     CDS             complement(24807..25322)
                     /locus_tag="JQH65_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY6137637.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPGNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPES
                     ARVASASTIPNRDARNIPLRVDLKQGDQSWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            25448..25771
                     /locus_tag="JQH65_000108"
     CDS             25448..25771
                     /locus_tag="JQH65_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAY6137638.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            25768..26598
                     /locus_tag="JQH65_000109"
     CDS             25768..26598
                     /locus_tag="JQH65_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAY6137639.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(26595..27605)
                     /locus_tag="JQH65_000110"
     CDS             complement(26595..27605)
                     /locus_tag="JQH65_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAY6137640.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMFSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLCSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     FRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGKL
                     PR"
     gene            complement(27704..29134)
                     /locus_tag="JQH65_000111"
     CDS             complement(27704..29134)
                     /locus_tag="JQH65_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY6137641.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(29145..30146)
                     /gene="ltaE"
                     /locus_tag="JQH65_000112"
     CDS             complement(29145..30146)
                     /gene="ltaE"
                     /locus_tag="JQH65_000112"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HAY6137642.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKVRNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(30183..31901)
                     /gene="poxB"
                     /locus_tag="JQH65_000113"
     CDS             complement(30183..31901)
                     /gene="poxB"
                     /locus_tag="JQH65_000113"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HAY6137643.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(32034..33002)
                     /gene="hcr"
                     /locus_tag="JQH65_000114"
     CDS             complement(32034..33002)
                     /gene="hcr"
                     /locus_tag="JQH65_000114"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HAY6137644.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVHTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAKAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(33014..34666)
                     /gene="hcp"
                     /locus_tag="JQH65_000115"
                     /gene_synonym="priS"
     CDS             complement(33014..34666)
                     /gene="hcp"
                     /locus_tag="JQH65_000115"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415394.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HAY6137645.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVSAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATIVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(34810..35709)
                     /gene="lysO"
                     /locus_tag="JQH65_000116"
     CDS             complement(34810..35709)
                     /gene="lysO"
                     /locus_tag="JQH65_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HAY6137646.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(36203..36898)
                     /gene="aqpZ"
                     /locus_tag="JQH65_000117"
     CDS             complement(36203..36898)
                     /gene="aqpZ"
                     /locus_tag="JQH65_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HAY6137647.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATAKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            37324..38982
                     /locus_tag="JQH65_000118"
     CDS             37324..38982
                     /locus_tag="JQH65_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HAY6137648.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAIEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(38979..39935)
                     /locus_tag="JQH65_000119"
     CDS             complement(38979..39935)
                     /locus_tag="JQH65_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 domain-containing protein"
                     /protein_id="HAY6137649.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLKLTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            40086..41201
                     /gene="macA"
                     /locus_tag="JQH65_000120"
     CDS             40086..41201
                     /gene="macA"
                     /locus_tag="JQH65_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HAY6137650.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAEIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            41198..43144
                     /gene="macB"
                     /locus_tag="JQH65_000121"
     CDS             41198..43144
                     /gene="macB"
                     /locus_tag="JQH65_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HAY6137651.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHMVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALVRE"
     gene            complement(43217..43441)
                     /gene="cspD"
                     /locus_tag="JQH65_000122"
     CDS             complement(43217..43441)
                     /gene="cspD"
                     /locus_tag="JQH65_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HAY6137652.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            43764..44084
                     /gene="clpS"
                     /locus_tag="JQH65_000123"
     CDS             43764..44084
                     /gene="clpS"
                     /locus_tag="JQH65_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HAY6137653.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            44115..46391
                     /gene="clpA"
                     /locus_tag="JQH65_000124"
     CDS             44115..46391
                     /gene="clpA"
                     /locus_tag="JQH65_000124"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HAY6137654.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(46735..46822)
                     /locus_tag="JQH65_000125"
     tRNA            complement(46735..46822)
                     /locus_tag="JQH65_000125"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46786..46788),aa:Ser,seq:gga)
     gene            complement(47076..47294)
                     /gene="infA"
                     /locus_tag="JQH65_000126"
     CDS             complement(47076..47294)
                     /gene="infA"
                     /locus_tag="JQH65_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAY6137655.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(47579..48283)
                     /gene="aat"
                     /locus_tag="JQH65_000127"
     CDS             complement(47579..48283)
                     /gene="aat"
                     /locus_tag="JQH65_000127"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HAY6137656.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(48325..50046)
                     /gene="cydC"
                     /locus_tag="JQH65_000128"
     CDS             complement(48325..50046)
                     /gene="cydC"
                     /locus_tag="JQH65_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HAY6137657.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMMVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAESL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(50047..51813)
                     /gene="cydD"
                     /locus_tag="JQH65_000129"
     CDS             complement(50047..51813)
                     /gene="cydD"
                     /locus_tag="JQH65_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HAY6137658.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQCGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(51936..52901)
                     /gene="trxB"
                     /locus_tag="JQH65_000130"
     CDS             complement(51936..52901)
                     /gene="trxB"
                     /locus_tag="JQH65_000130"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAY6137659.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            53446..53940
                     /gene="lrp"
                     /locus_tag="JQH65_000131"
     CDS             53446..53940
                     /gene="lrp"
                     /locus_tag="JQH65_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HAY6137660.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            54075..58085
                     /locus_tag="JQH65_000132"
     CDS             54075..58085
                     /locus_tag="JQH65_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAY6137661.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDEEEIIYTA
                     RGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWAAPVEPV
                     TQTPPVTSVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQYNEPLQ
                     QPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPAPEQPVA
                     GNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKP
                     ARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSAAAVPPVEAAA
                     AVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTR
                     RELASYGIKLPSQRAAEEKAREARRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQR
                     YGEQYQHDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMK
                     ALLDDGPHEPLFTPIVEPVQQPQQLVAPQQQYQQPQQPVAPQQQYQQPQQPVAPQPQY
                     QQPQQQVAPQPQYQQPQQPVAPQQQDTLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPP
                     PSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGVKAARIS
                     NLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRDNPSPLT
                     VVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDVRFIMID
                     PKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNLAGYNEK
                     IAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKKVEELIA
                     RLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILDQAGAES
                     LLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSDSESEGG
                     AGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEAQGIVSE
                     QGHNGNREVLAPPPFD"
     gene            58240..58851
                     /gene="lolA"
                     /locus_tag="JQH65_000133"
     CDS             58240..58851
                     /gene="lolA"
                     /locus_tag="JQH65_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HAY6137662.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            58862..60205
                     /locus_tag="JQH65_000134"
     CDS             58862..60205
                     /locus_tag="JQH65_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAY6137663.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPREIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            60296..61588
                     /gene="serS"
                     /locus_tag="JQH65_000135"
     CDS             60296..61588
                     /gene="serS"
                     /locus_tag="JQH65_000135"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAY6137664.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            61827..64271
                     /gene="dmsA"
                     /locus_tag="JQH65_000136"
                     /pseudo
     CDS             61827..64271
                     /gene="dmsA"
                     /locus_tag="JQH65_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            64282..>64618
                     /locus_tag="JQH65_000137"
     CDS             64282..>64618
                     /locus_tag="JQH65_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="oxidoreductase, Fe-S subunit; terminal electron
                     transfer protein for the reduction of DMSO; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit B"
                     /protein_id="HAY6137665.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYG"
BASE COUNT        15881 a        16218 c        17411 g        15108 t
ORIGIN      
        1 acttaacgcc gtgttggatt acattgacgc ggtggcggca acagatacca gcaccgcgcc
       61 ggatgtcatc tggcctgaac tgccggaggc gtaggccatt caatatctgg agcactggag
      121 gtatcaatca gttccagtgc gtccagataa tccagccaca aattatattg cgccagttcc
      181 tcacctttca gacgaccaat agcggcttta ccgggccatt gtttactgtt catgtattcg
      241 ttggcctgat taagcaataa ttgcctttct gattctgcct gttgaataag ttcttcatat
      301 gatggtggag gtattagtgc ccatgtgggt aatccatttt ttcctgcaac acgaattttg
      361 tcatctgggg gcgtattgat tgcaaattca ttataaacat catcactgac agccagagca
      421 tcatctggcc atgaatttgc attaatgtaa tcatccttaa gcgcaggatt cacaaaaatg
      481 tttaaagatg gactataaaa catattaaac ccctatagcg atataacgac ctaatacagc
      541 atttgcagca gtcgctatgc ttgaaaaacc gcggaactga tttgatgtaa ttgcagaagc
      601 cgacaggatt cctgcgcctg aaggtgtatg ccccacatga ctaactatca tcccataaca
      661 tgctgacgga aaggcaaaag gaaaatcatt aagatatcct gcatcttcac caccagaccc
      721 accaaatctc gcctgccccc actgaataat cagtgttctc cgggaacctg aaataattaa
      781 cggaatcgtt acatacccat tcaggccaat gacacccgat gcagtgccag ccagagataa
      841 ttctcccaaa ccaacgttta agaaaatgca gagataatgg ctaactggca tcatccccgg
      901 tttttattca ggggattgat catgcttatt ggctatgtac gcgtgtcaac aaatgaccag
      961 aacaccgatt tgcaacgtaa tgcactgaac tgcgcgggat gtgagcggat ttttgaggat
     1021 aaaatcagtg gcactaagcc cgacagaccg gggcttaaaa aactgctcag gacactatcg
     1081 gcaggagaca cgctggttgt ctggaagctg gacagactgg gacgcagtat gcggcatctt
     1141 gttacgctga tagaagagtt gcgccagcgt ggtgtgaatt tccgaagcct gactgacagt
     1201 attgatacca gtaccccaat gggccgtttc ttttttcacg tcatgggggc tctggctgaa
     1261 atggaacgtg aactgatagt tgaacgtacc agagcagggc tggctgcagc tcgtgccaaa
     1321 ggcagagtag gtggacgccg tcctaagttg accaccgaac agtgggcaca gattggacgt
     1381 ttactcgagg ccggagaatc aagacagcgt attgcactga tttttgatgt gggcgtttct
     1441 accatttata gaaaatttcc ggcgaatgag agcaatgaat cgccctgagt cagtattatt
     1501 ttgatttttc ctgtaagcgg acaaataccg tcattttgtg tgaataacga cacaactgcg
     1561 cttagctgtt tgtcaggcac aatcacttca acatagggcg aagcctaatc caatcaggag
     1621 gttcgccact atggctcagg attaccacca cggggtgcgc gttgttgaag tcaacgaagg
     1681 cacccgatcc attaccacgg tgagcaccgc catcgtgggt atggtctgca cgggcgatga
     1741 tgccgatgca aaaatgtttc ctcttaataa acctgtgctg atcactgatg tgctgaccgc
     1801 cagcggtaaa gcgggtgagt ctggcacgct ggcccgttcg ctggatgcca tcgctgacca
     1861 ggcaaaaccc gtgaccgttg ttgtgcgtgt gccgcagggt gaaacggaag aagaaaccac
     1921 aaccaatatc attggcgcag tgaccgccga gggtaaaaaa acaggtatga aagccctgtt
     1981 atctgcccag tcacagctcg gcgttaaacc gcgcattctc ggcgtgccag gtcacgacac
     2041 gaaggcggta gctactgagt tgctgagcgt ggcgcaaagc ctgcgtggat ttgcttacct
     2101 gtcagcgtat ggttgcaaga cggtgcagga agcaatcact taccgtgaaa acttcagtca
     2161 gcgtgaagga atgctgatct ggcccgactt tactggctgg gacacggtgc tgaatgccga
     2221 agcaacggct tatgccaccg cccgtgcgct cggtctgcgc gctaaaattg atgagcagac
     2281 cggatggcac aaaagcctgt ccaacgtggg cgtgaacggt gtcaccggaa tttctgctga
     2341 tgtgttctgg gatctgcagg acccggcaac cgatgcaggt ctgcttaacc agaacgatat
     2401 caccacgctt atccgcaaag acggtttccg cttctggggt tcccgctgtc tgagcgatga
     2461 tccgctcttt gccttcgaaa actacacccg cacggcgcag gtgctgatgg acacgatggc
     2521 agaagcgcac atgtgggcgg tggacaaacc gctgaacccg tcgctggccc gcgacattat
     2581 cgaaggtatc cgcgccaaaa tgcgtagcct ggtcagtcag ggctatctca ttggtggtga
     2641 ttgctggctg gacgagtcgg tgaacgataa agacacgctg aaagccggga aactcaccat
     2701 cgattacgac tacacgccag tgccgccact tgaaaacctg atgctgcgtc agcgcatcac
     2761 cgatcagtac ctggtgaatt tctccagcca ggtcagcgcg taaggggaca acatggcttt
     2821 accacgcaaa ttaaaacacc tgaacctgtt taacgacggg aacaactggc aggggatcgt
     2881 tgagtcgctg acgctgccga aattcacccg caaatatgag aagtatcgcg gcggcggtat
     2941 gcccggagcg gtggatgtgg atctgggact ggatgacagt gcactggata cagaattttc
     3001 cattggtggt actgaactgc tgctgtttaa gcagatgggc aaagccacgg tggatggcat
     3061 ccagctgcgc tttaccggct ctattcagcg tgatgatacc ggggaagtgc aggccgtgga
     3121 gcttgtggtg cgtggacgtc acaaagaagt agattccggc gagtggaaga cgggcgaaag
     3181 caacactacc aaagtgacca gtaccaacag ctacgcgaag ctgaccatca atggtgaggt
     3241 gctctatgaa gtggacctga tcaacatggt ggaaattgtg gacggtgtgg acctgatgga
     3301 agcgcaccgc aacgccctcg gcctctgatg tatctgaacg gcgcggaatg ccgcgccaga
     3361 acctaattta caggacaaca aaatgagcga taagcagact gaaaagacca ttcaactgga
     3421 tactcccatc atgcgcggta aaacagaaat taccgaaatt gtgttgcgta aaccgcagtc
     3481 cggtgcgctg cgcggcacac gcctgcaggc cattatggat atggatgtga acgcgatgat
     3541 gaccgtgatc ccccgcatct ccagtccggc actgactgca caggaaattg cagagatgga
     3601 cccggcagat ctcactgcca tgtcggttga ggttgtcact tttttgttga agaagtcggt
     3661 gcttgccggt ttaccgacag cctgacggtt gacgatctgg tggctgatat cgccaccatc
     3721 tttcactggc cgccatccgt tactgacgtt atgccgctga ccgaagtgct ggaatggcgg
     3781 gataaagcga ttcagagaag cggggccagc catgagtgac aataacctgc gtctgcaggt
     3841 gattcttaat gcggttgaca aactcacccg cccatttcga tccgcgcagg ccagttcaag
     3901 agaactggct gctgctgtca aaaaatcccg cgatgcaata aagcagcttg atcaggccgg
     3961 gagcagtctg gacagcttcc gaaagctgca ggcagaaaat cagaaattag gcgacaggct
     4021 gaactatgcc cgccagcgtg caaatttgct cagtcaggaa ctgggagcga tggggccgcc
     4081 ttcgcaacgt caggttgttg ctctgggccg tcaacggctg gctgttcagc gcctagaaga
     4141 acgccagaaa aagctgcagc agcagacggc gcttgtgcgt gcagaacttt atcgtgctgg
     4201 tatttcagct aatgatggcg ccagtgcgac ggcccgcatt acccgtgaaa cgatgcgtta
     4261 taacaggcag ctttctgagc aggaagcgag gttacgacgt gtcggggagc aacagcgaaa
     4321 aatgcacgcc gcccgggggg cttacgccag gcgtcttgag gtaagggatc gtattgcagg
     4381 tgccggagcc accaccacgg ctgcagggct ggcaatgggc gcaccagtga tggcggcagt
     4441 aaaaagctat accagcatgg aagatgccat gaaaggtgtg gcaaagcagg tcaatggtct
     4501 gcgtgacgat aatggcaacc gcactgcgcg tttttacgaa atgcagggtg ccatcaaggc
     4561 tgccagcgaa cagttgccga tggaaaacgg tgctgtggac ttcgccgcac tggttgaagg
     4621 tggtgcgcgc atgaacgtcg caaaccctga cgacagctgg gaagaccaga aacgtgacct
     4681 gctggccttc gccagcacgg cggcaaaggc ggcaacagcc tttgaactgc cagcggatga
     4741 actgtcagaa agtctgggga aaatcgccca gctctacaaa atccctaccc gcaatattga
     4801 gcagctcggt gatgcgctga actatctgga tgataacgcc atgtcgaaag gggcggacat
     4861 cattgatgtc atgcaacgcc tgggcggtgt ggctgaccgt ctggattatc gtaaagcggc
     4921 ggcgctgggt tccaccttcc tgacactcgg cgctgcgcct gaggttgcag ccagtgcagc
     4981 aaacgcgatg gtgcgtgaat tgtccattgc caccatgcaa agcaagagtt tctttgaagg
     5041 gatgaatctg ctgaaactca atccagaagt gattgaaaag cagatgacga aggatgcgat
     5101 gggaactatc cagcgggtgc tggagaaggt gaatgcgctg ccacaggata agcgcctgtc
     5161 tgccatgacc atgttgtttg gtaaagagtt tggcgatgac gcagcgaaac tggcaaacaa
     5221 cctgccggaa cttcagcgcc agttaaaact gactgcgggc aatgatgcgc tcggttccat
     5281 gcagaaagaa tccgacatca acaaggattc actttctgct cagtggttgc tggtcaaaac
     5341 cggagcacag aacaccttca gcagcctggg ggaaacgctg cgccagccgc tgatggatat
     5401 tctgtacacg gtgaaaagcg tcacgggggc gttgcgtcgc tgggtggaag ctaacccgga
     5461 actgacgggc actctgatga aagtagccgc tgtggtagct gccgttaccg tgggcctcgg
     5521 caccttagcg gtggcgctgg ctgcagtgct ggggccgctg gcagtcatcc gtctgggatt
     5581 ctctgtgctg ggtatcaaaa cgttaccttc cgttacggca acagtaaccc gaaccagcaa
     5641 cgcgttgtcc tggctggctg gcgcaccact ggcactgctg cgacgcgggc ttgcttcatc
     5701 gggcaacgct gcaggtttac ttactgcgcc gttgtcgtct ttgcgccgca cggcatcact
     5761 gacgggaaat gtcctgaaaa ctgtagcagg tgcgccggtt gcacttttgc ggtctggatt
     5821 atccggttta cgtgcggttg ctgtgatgtt tatgaatcca ctggcggtac tacgcggcgg
     5881 actggctgcc acaggcacgg tgctgcgtat gctcgcatcc ggtccactgg cgatgctgcg
     5941 cgttgccctg tatgccgtct ctggtctgtt aggtgctctg ctcagtctga taggtcttgt
     6001 ggttactgca ctggcgggtg tggcgctggt tgtctggaaa tactggcagc ccatcaccgc
     6061 atttctcggt ggcgtggtgg aaggattcaa agcggcggca ggtcctgtca gtgccgcgtt
     6121 cgaaccgctt aagcctgtgt tccagtggat tggcgacaaa gtacaggcgc tgtggggctg
     6181 gtttactgat ctgctgacgc ccgttaagtc gacctctgcc gaactgcaga gtgcagcggc
     6241 aatggggcga cgattcgggg aggctctggc ggaagggctg aatatggtca tgcatccgct
     6301 ggactccctg aaatccggcg tttcctggtt gctggagaaa cttggcattg tcagtaaaga
     6361 ggccgcaaag gcgaaactgc cggaaagcgt gacgcgtcag caacctgcga cggtgaatgc
     6421 agacggtaaa gtgatgatgc catcgggtgg ttttccgtca tggggatatg gctttgcggg
     6481 gatgtatgac agcggcgggt atatcccgcg cgggcagttt ggcatcgtcg gtgaaaacgg
     6541 gccggaaatt gttaacggcc cggcaaatgt gaccagccgg agaaatacag ctgcactggc
     6601 tgccgttgtt gccggaatga tgggtgttgc tgctacgcca gcagagcttc caccgttgca
     6661 ccctttggca cttcccgcga aaggcggtga agcgatggtg agtcgtgcag ccactgtgcc
     6721 gcccgttcaa cggattgagg caccgatgca gatcatcatt caaacgcagc caggacaaag
     6781 tgcgcaggat attgcgcggg aggtggcccg ccagcttgat gaacgtgaac gcaggctgaa
     6841 ggcaaaagcc aggagtaact acagcgatca ggggggatac gacgcatgat gatggtgctg
     6901 ggattgtacg tgtttatgct gcgcaccgtg ccgtatcagg agctgcagta tcaacgcagc
     6961 tggcgacatg cggcaaacag ccgggtaaac cgacgcccgt caacgcagtt tctgggaccg
     7021 gacaacgaca tgctgacgct ttctggtgtt cttatgccgg aggtaacggg cggcaggctg
     7081 tcgttgctgg ctctggagca gatggcagaa cagggaaaag catggcccct gattgaaggc
     7141 agcggcacga tttacggcat gtatgtgatt gagggactga atcagactaa aacggagttt
     7201 ttccgcgacg gtatgccgcg ccggattgag ttcaccctgt cgctaaaacg cgtggatgaa
     7261 tccctgtccg atatgttcgg tgatctcagt gcgcaactga ataatctgca gggaacggca
     7321 acatctgcct taagcgatat cagtaaaacg gtgggagggc tgctgtcgtg aatttcagct
     7381 ctgaactgct taacaaaggc aacaaaactc cggcattcag catcagtatt gaaggcaggg
     7441 atatcaccac tgtgctggac aaccgcctga tggggctgac gctgacggat aaccggggct
     7501 ttgaagcgga ccagcttgat ctggagctgg acgacgccga tggaaaaatc gtgctgccgc
     7561 gccgtggggc tgtcattacg ctggcgctgg gctggaaggg acagccgctt ttcccgaaag
     7621 gggcattcac ggtggacgag attgaacaca ctggcgcacc ggatcgcctg actatccggg
     7681 cgcgaagtgc tgattttcgt gaaacgctga atacccgccg tgaaaagtcg tggcacaaga
     7741 ccaccgttgg ggaagtggtg aaggaaatag ctgcgcggca caaactgaag atggcattgg
     7801 gtgaagactt gtcggataaa ctcgtggagc atatagacca gactaatgag agtgacggca
     7861 gttttttgat gcggctggcg cgccagtacg gtgctattgc gtcggtgaaa aatggcaatc
     7921 tgttattcat ccggcagggg cagggtaaaa gcgccagcgg taaaccacta ccggtgatca
     7981 ctatcacacg caaggacggc gacagtcacc gctttaccct ggcagatcgc ggagcttaca
     8041 cgggcgtcat tgccagctgg ttgcataccc gcgaacccgc gaagaaagaa agcaccacgg
     8101 tgaagcgtaa gcgcaggact aagaagcaga agaaagagcc ggaagcgaag cagggcgatt
     8161 acctggtggg tacggatgaa aacgtgctgg tacttaatcg cacttatgcc aaccggagca
     8221 acgccgaacg ggcagcgaaa atgcagtggg aacgcctgca acgcggcgtt gcatcattct
     8281 cgctacaact ggcggaaggg cgggcagatc tctacacaga aatgcctgtg aaggtcagtg
     8341 gctttaaaca gccgctagat gatgcggaat ggaccattac gactctgacg catactgtca
     8401 gcccggataa cggttttacg accagtctgg agcttgaagt gaggattgat gatttcgaaa
     8461 tggaatgatt cttcgcaatg gagaactttt aagtttgcaa aatggaataa tgcggtatca
     8521 ttattgtgaa tttagcaaaa atggggagag ctcgaaaaat gatgatttgc ccactgtgtg
     8581 gaagtgccgc ccatactcgc agcagttttc aggtatcttc attgaccaaa gagcgttaca
     8641 accagtgcca gaacattaac tgcagccata cttttgttac ccatgaaact tttgttcgtt
     8701 cgattgcaac gccaaaagag tcaaatccgg ttcagccgca tccaatgaaa tcaggacagg
     8761 tggcgctctc tctttgacgc tgccgccatt ttgtcgccat cgttaaaaaa cagtgcttct
     8821 aacatcatga ttttaaaagg cataaatttc agacaacaaa aaaaccatca accttgaacc
     8881 gaaatggcgg ggttgatggg ctccacaaaa tggggacatc aaagaaaagc agtggcacta
     8941 attaagactg atgccctgcg gaaaagttct gcggttgtgc aaaaaaattt cattttcagg
     9001 gcaacttcag ttttatccta atcctggcca taccatgacg atgattgtcc ctgccagcgt
     9061 cagcaggacg ttggcgattg cgtaggtgcc cgcatagccc agcgcaggga tgttactgtg
     9121 agctgtatca ctgatgatct ccattgccgg cgcgcaggtg cgtgcgccca tcattgcgcc
     9181 gaacaacagt gcgcggttca ttcgcaatac ataagcaccg aacaagaaac agataaccac
     9241 gggcacaaga ctgacaatta atccggcaat caacatctga ccgccaatcg cgcccaggcc
     9301 gttattaata ccgctaccgg cgctcagacc aacgcctgcc ataaacacca tcaagccgaa
     9361 ctctttcacc atgcttaatg caccttgcgg aatgtaaccg aaggtcgggt ggttagcacg
     9421 cataaagccc agcataattc cggcgaataa caacccggca gcgttcccca tgccgaaact
     9481 gaatgtgctg aactggaagg tgatcatccc gatcatcagc ccaataacaa agaaggcgca
     9541 aaatgccagc aggtcagtga cctggctgtg aatcgagata aagccgatgc gatcggcgat
     9601 ggtttttacg cgacgggcat caccgctgac ttgtaaaacg tcacctttgt taagcacgac
     9661 gttgtcatct atcggcatct caatctggct acgaatgacg cggttaagga agcaaccgtg
     9721 atcggtcaac ttcagttgtg cgagacgttt acctacagcg ttatggtttt taacgaccac
     9781 ttcttcagtg acgatacgca tgtcgagaag gtcacgatcg aaaacttctt taccgttacg
     9841 gaagctggga tcgagtcggg catgggcgtc gggatagcct accaacgcta tttcatcgcc
     9901 catttgtagc acggcatcac cgtctggatt tgccagaatc ccgttacgtc gaatacgttc
     9961 aatgtagcag ccggtttgtc gataaatacc cagttcacgc agatttttgc cgtcggtcca
    10021 ggccaccagc tccgggccga cgcgataggc gcggatcacc ggtaaataaa ccttacggtt
    10081 ggcatcagtg tccaggccac gttcgcgggc gatttgctgg gcgctggtct gtaagtcctg
    10141 atgctgcaat ttcggcaagt aacgcgcacc aacaatcaaa ctcaccagac cgattaaata
    10201 ggttaaggca tacccgaggc tcagattatc cagtgccagt gagagctgcc tgctttccat
    10261 gccggaatga cgcagtgtat cgccagcacc gaccagaacc ggtgtcgacg tcatagagcc
    10321 tgctaacata ccggccgtca ggccaatatc ccagccaaac agcttaccta accctaaggc
    10381 gatcaccagc gcactgccaa ccatcaccag tgctaacatt aggtaatttt tcccatcgcg
    10441 aaaaaaaatg gaaaaaaagt tcggtccggc ttcgaccccg acgcagaaaa taaacagcat
    10501 aaagccaaga ttaagcgcat cggtgttaat gctgaaatgt tgttggccta ataacagcga
    10561 tacgactaaa acgccaatgg aattacccag ttggatcgaa ccaagtcgta actttccgag
    10621 acatagccca agcgcgagga ccacaaataa taacagaatg taattcccat ttaacaattc
    10681 ggcgacgttt atattcacgg aggctaactt cttgtttact agtaagctgt tgaaagaaat
    10741 ggtaatttac gataatgttt tttaccagaa ttcagggcgc agattcattc agcgcaccta
    10801 aacgatagta aagtaacaat atattttact agtgtaatca cattaggtat caacggctat
    10861 atgaattgcg ttggcctata ttagcatgga atgcgaagcg gctttatctt actgaacgcc
    10921 acactggcga aaaatgtgtt cgatagacgc agtgtcagga ggaacgagtg aaacataaac
    10981 aacgttgggc gggggcaatc tgctgttttg tcctcttcat tgtggtgtgc ctttttctgg
    11041 cgacgcacat gaaaggcgct tttcgggctg ccgggcatcc tgaaatcggc ttgctgtttt
    11101 tcattcttcc tggagcagtt gccagtttct tttcacagcg tagagaagtc ctgaaacctc
    11161 tatttggcgc aatgctggcg gcaccctgtt caatgctcat tatgcggctg tttttttcac
    11221 cgacgcgctc attctggcaa gagctggcat ggttactaag cgcggtgttc tggtgtgcgc
    11281 tgggggcact gtgtttctta tttatcagta gtttgtttaa accacagcac agaaaaaatc
    11341 agtaaagccc tcaacgcgag ggcttgtcag acgatcaggc gtccagattt tctttcaccc
    11401 atgcagcaaa atcggtatag ccgccgatat gttgctgatc gacaaaaatc tgcggcacgg
    11461 tttctacggg tttacctgcc ttttgttgta gatcttcttt agtgatccct tccgcacgaa
    11521 tatctacata ctgatactga aaatcatcgc gttcattgct caatttctca gccagatctt
    11581 ttgcacgcac acagtaaggg caacccgaac gaccaaaaat aacggtttgc attatttctc
    11641 tcctcataga tttatgcctg taatgatcac gctaaaatgt attcgctgaa agtaggttta
    11701 acctgttgca ttaattgcta aaagctataa ctgttaaaca caatacagtg aaaagtttta
    11761 gactgaaggc tcactttgca gagggaagcg tatgcgcgcg atcggtaaat tgcctaaagg
    11821 cgtgttgata ctggaattta tcggaatgat gctactggcg gtggcgctgc tgtcggtaag
    11881 cgactccctg tcgctgcctg agccattttc tcggccagaa gtgcagattc tgatgatttt
    11941 tctcggtgtt ttgctcatgc ttcccgctgc ggtggtggtt attcttcagg tggcaaaacg
    12001 tcttgcccca cagctgatga accgtccacc gcaatattca cgttcagaaa gagaaaaaga
    12061 taatgacgcc aaccattgaa cttatttgtg gccatcgctc cattcgccat ttcactgatg
    12121 aacccatttc cgaagcgcag tgtgaggcga ttattaacag cgcccgtgcg acgtccagtt
    12181 ccagtttttt gcagtgtagt agcattattc gcattaccga caaagcatta cgtgaagaac
    12241 tggtgacgct gaccggcggg caaaaacacg tagcgcaagc ggcggagttc tgggtgttct
    12301 gtgccgactt taaccgccat ttacagatct gtccggatgc tcagctcggc ctggcggaac
    12361 aactgttgct cggtgtcgtt gatacggcaa tgatggcgca gaatgcatta accgcagcgg
    12421 aatcgctggg attgggcggg gtatatatcg gcggcctgcg caataatatt gaagcggtga
    12481 cgaaactgct gaaattaccg cagcatgttc tgccgctgtt tgggctgtgc cttggctggc
    12541 ctgcggataa tccggatctc aagccgcgtt taccggcctc cattttggtg catgaaaaca
    12601 gctatcaacc gctggataaa gacgcactgg cgcagtatga cgagcaactg gcggaatatt
    12661 acctcacccg tggcagcaat aatcgccggg atacctggag cgatcatatc cgccgaacaa
    12721 tcattaaaga aagccgccca tttattctgg attatttgca caaacagggt tgggcgacgc
    12781 gctaaaaccg ccacgtcgat gtatgatacg cgggcttttg accaggtctg acagagaggt
    12841 gcagggtgaa aattgccata ttgtcccggg atggaacgct ctattcgtgt aagcggctgc
    12901 gtgaagccgc tatacagcgc ggtcacctgg ttgaaattct tgatccgctt tcttgctaca
    12961 tgaacataaa tcctgcggcg tcttctattc actacaaagg ccgcaagtta ccccattttg
    13021 acgcagtgat cccgcgtatt ggcaccgcca ttacctttta tgggacggcg gcactgcgcc
    13081 agttcgagat gctggggagc tatccgctca atgagtcggt cgccattgcc cgggcgcgtg
    13141 acaaattgcg ttccatgcaa ctgctggcgc gtcagggcat cgacctgcct gtcacgggca
    13201 ttgtgcattc gccggatgat accagcgatt taatcgacat ggtcggtggt gcgccgctgg
    13261 tggtcaagtt ggttgaaggc acgcagggaa ttggcgtcgt gctggcggag acgcgtcagg
    13321 cggcggaaag cgtgattgac gctttccgcg gtctgaacgc gcatattctg gtgcaggaat
    13381 atatcaaaga ggcgcaaggg tgcgatatcc gctgtctggt tgttggcgat gaagtggtcg
    13441 ctgcgattga acggcgggcg aaagagggcg attttcgttc caatttgcat cgtggcggcg
    13501 cggcaagcgt cgccagtatc acaccacagg agcgtgaaat cgcgataaaa gccgcgcgaa
    13561 cgatggcgct ggacgttgct ggtgtggata ttctgcgtgc taatcgcggg ccgttggtga
    13621 tggaagtgaa tgcgtcgccg gggctggaag gaatagaaaa aaccactggt atcgacatcg
    13681 cgggtaaaat gatccgctgg atcgaacgct acgctacgac agaatattgc ctgaaaacgg
    13741 gtggttagtc gcaatcacat tactgatcat ggttttgcct gcgctttttg cgtaagctgt
    13801 gccggtcttt ttatcgaaag aggttgtaca aaattatgac atcgctggtc gttcctggtc
    13861 tggatacgct gcgtcaatgg ctcgatgacc tggggatgag tttttttgaa tgtgataact
    13921 gtcaggctct gcatctgccc catatgcaga atttcgacgg tgtctttgat gccaaaatcg
    13981 atctgatcga taacacgatc ctgttttctg ccatggcgga agtccgacct tcagccgtat
    14041 tgccgctggc ggcggattta tctgccatca atgccagttc gctgaccgtg aaagcatttc
    14101 ttgatatgca ggatgataat ctgccaaagc tggtggtttg ccagtcttta tccgttatgc
    14161 agggcgtaac ctacgagcag tttgcatggt tcgtgcgtca gagcgaagag cagatttcga
    14221 tggtcattct tgaagctaat gcccatcaac tgctgttacc gactgacgat gaagggcaaa
    14281 acaacgttac cgaaaactat ttcctccact gataactcct ttcgagcacg cagtcgctgg
    14341 tgcagtgact gcgcactgca aaattatctg ctgtttttaa ccttttctta aagattattt
    14401 cacttctctt gtgccgattt ggctttatca catagagcaa atacgcataa caatttgtta
    14461 aataccgttt tttaatccga gctatagtct caaaccctgg ctaaagttat tcttgcgatg
    14521 cttttatata gcgagcagtg ctggccggga gagagttatc ttttcttaca ccgcgccgat
    14581 aaaaaatatg cacgtttatt gcatatcttt tagtatgaca acttttgttc gtttgttaac
    14641 gaactttcag aaggaaagag atatgaccgc cttaaataaa aaatggcttt cgggtctggt
    14701 tgcgggtgct ctgatggccg tctctgtcgg cacgctcgcg gctgaacaaa aaacactcca
    14761 catttataac tggtctgatt atatcgcccc tgacacggtg gccaattttg aaaaagaaac
    14821 cggtattaaa gtcgtctacg atgttttcga ctctaacgaa gtactggaag gcaaattaat
    14881 ggcgggtagc accggctttg acttggtggt tccatctgcc agctttcttg aacgccagtt
    14941 gactgcggga gttttccagc cgctggacaa aagcaaattg ccggagtgga agaatctcga
    15001 cccggagctg ctgaagctgg tcgccaaaca cgatcccgac aataaatttg ctatgcccta
    15061 tatgtgggcg acgaccggga ttggctataa cgttgataaa gttaaagctg tgctgggcga
    15121 aaacgcgccc gtcgatagct gggacttgat cctcaaacct gaaaatctgg aaaaactgaa
    15181 aagctgcggt gtctctttcc tggatgcgcc agaagaagtt tttgctaccg tgttgaatta
    15241 tctcggcaaa gatcccaaca gcactaaagc ggatgattac accggaccgg caaccgatct
    15301 gctgttaaag ctgcgcccga acattcgtta tttccattca tctcaataca ttaacgacct
    15361 ggcaaacggc gatatttgcg tcgctatcgg ctgggcaggt gatgtctggc aggcgtcaaa
    15421 ccgcgcgaag gaagcgaaga atggagtgaa tgtctcgttc tcgattccaa aagaaggggc
    15481 gatggcgttc tttgatgtat tcgccatgcc tgcggatgcc aaaaacaaag acgaagccta
    15541 tcagttcctg aattacctgc tgcgcccgga tgtagtagcg catatttccg accatgtgtt
    15601 ctatgccaac gccaataaag cagccacgcc gctggtgagt gcggaagtcc gtgagaaccc
    15661 gggtatttat cctccggcgg acgttcgtgc gaagctattc actctgaaag tacaggatcc
    15721 gaaaatcgac cgtgtgcgca cccgcgcgtg gaccaaagtg aagagcggaa aataatccgc
    15781 agccttagat gccgggcggg cgcaccacac ccgccggcaa ttcgcaccat catggtgcgc
    15841 ttgcacacat tcaatgccgg agagcagccg tgaatgacgc tatccctcgc ccgcaggcga
    15901 aaacccgtaa ggcgctgacg ccgctattag aaatccgcaa cctcaccaaa tcctacgatg
    15961 gtcagcatgc ggtcgatgat gtcagcctga ccatctacaa aggtgaaatc ttcgcgctgc
    16021 tgggcgcttc cggctgtggc aagtccacgc agctgcgtat gctggcaggt tttgaacaac
    16081 cttctgccgg gcagataatg ctcgatggcg tcgatttgtc acaggtgccg ccttacctgc
    16141 gccccatcaa tatgatgttt cagtcttacg cgctgtttcc gcatatgacc gtggaacaga
    16201 acatcgcttt tggcctgaaa caggacaaac taccgaaagc ggaaattgcc agtcgggtcc
    16261 atgagatgct cggcctggtg cacatgcagg agttcgccaa acgcaaaccg catcagcttt
    16321 ccggtggtca gcgacagcgt gtggcgctgg cccgaagcct tgccaagcgc ccgaaactat
    16381 tactgctcga cgagccgatg ggcgcgctgg ataaaaagct gcgtgaccgg atgcagcttg
    16441 aagtggtgga tattctggag cgtgtcggcg tgacctgtgt gatggtcact cacgatcagg
    16501 aagaggcgat gacgatggcg ggacgtatcg ccattatgaa tcgcgggaaa tttgtccaga
    16561 ttggcgagcc agaagagatc tacgaacatc caaccacccg ctacagtgct gaatttatcg
    16621 gttcggttaa tgtctttgaa ggtgtactca aagagcgtca tgaggatggg cttgtgcttt
    16681 attcgcctgg gctggtgcat ccactgaaag tcgacgcgga tgcctcggtg gtcgataacg
    16741 tgccggtaca tgtggcgctg cgcccggaaa aaatcatgct ttgcgaagag ccgcccgcca
    16801 atggttgtaa cttcgcggtg ggggaggtga tacacattgc ctatctcggc gatctttcgg
    16861 tgtatcacgt tcgtctgaaa agtgggcaga tgattagcgc ccagctacaa aacgcccatc
    16921 gtcatcgtaa agggttaccg acctggggcg acgaagtgcg tttgtgctgg gaagtggaca
    16981 gctgtgtggt gctgacggtt taaggagcaa agatgaatac acttgaacct gctgcccagt
    17041 cgaaaccgcc gggcggattt aagctgtggt tgtcgcagct gcaaatgaag catgggcgca
    17101 aactggtcat tgcgttgcca tatatctggt tgatcttgct gtttctgctg ccatttctga
    17161 ttgtctttaa aataagcctg gcggagatgg cgcgcgctat tccaccttat accgaactga
    17221 tggagtgggc tgacgggcaa ctgtccatca ctcttaatct cggtaatttt ctgcaactga
    17281 ccgacgatcc gctctatttc gatgcttatc tccagtcgct acaggtggct gcgatttcga
    17341 cattttgctg tttactgatc ggctatccgc tggcgtgggc ggtggcgcac agtaagcctt
    17401 cgacccgtaa tattttatta ctactggtga tcctgccgtc gtggacctcg tttctgatcc
    17461 gcgtttatgc ctggatggga atattaaaaa acaacggtgt gctgaataat tttctgctgt
    17521 ggctgggggt tatcgatcaa ccgctgacca ttcttcatac caatctggca gtttatattg
    17581 gcattgttta cgcctacgtg ccgtttatgg tgctgccgat ttataccgcg ttgattcgta
    17641 ttgattattc gctagtggaa gcagcgctgg atctcggtgc acgaccgctg aaaacgttct
    17701 ttactgtgat cgtgccgctg actaaaggtg ggattattgc cggatcgatg ctggtgttta
    17761 tcccggctgt gggcgagttt gtgatcccgg aactgctcgg tggcccggac agcatcatga
    17821 tcgggcgcgt gctatggcaa gagtttttca ataaccgcga ctggccggtg gccgcggcgg
    17881 tagcgatcat catgttgctg ctgctaattg tgccgataat gtggttccac aaacaccagc
    17941 aaaaaagcgt gggagaacac ggatgaataa tttaccggta gttcgttcgc cctggcggat
    18001 tgtgattttg ctgctgggct ttacctttct ctacgcgcca atgctgatgc tggttatcta
    18061 ttcgtttaac agctcgaagc tggtgacggt gtgggccggc tggtcaacgc gctggtatgg
    18121 tgagttattg cgcgatgacg cgatgatgag tgcggttggt ttaagcctga caattgctgc
    18181 ctgtgcggca acggcggcgg cgatcctcgg gactattgcg gcggtggtgc tggtgcgctt
    18241 tggcaggttt cgcggatcaa atggctttgc ctttatgatc acagcgccgc tggtaatgcc
    18301 agatgtcatc acgggcttgt cgctgttgtt attattcgtc gcgcttgctc atgccattgg
    18361 ctggcctgcg gaccgcggta tgcttaccat ctggctggcg catgtcactt tctgtacggc
    18421 ttatgtggcg gtcgttattt cgtcgcgtct gcgggaactg gatcgctcga tagaagaagc
    18481 agcgatggat ctcggtgcga cgccgctgaa agtgtttttc gtcattacgc taccgatgat
    18541 catgcctgcg atcatttctg gctggttact ggcttttact ttgtcgcttg atgatctggt
    18601 gatcgccagc tttgtttccg ggccgggagc caccacgtta ccgatgctgg tcttttccag
    18661 cgtgcggatg ggggtgaatc cggaaatcaa cgcccttgca acgttaattc tcggtgcggt
    18721 cggaattgtc ggatttatcg cctggtatct gatggctcgc gcagaaaaac agcggatacg
    18781 cgatatccag cgtgcaagac atggctgaag acactaaaat ttgccaacct ggctacataa
    18841 tgccgcgcat gccgcggcat tgttttcatg gaagacgaaa cgttgggatt ttttaagaaa
    18901 acatcttcat ctcatgctcg cctgaatgtg cctgcgctgg tgcaggtagc ggcgctcgcc
    18961 attattatga tccgtggcct cgacgtgctg atgattttca atacgctggg tgtgcgcggt
    19021 attggcgagt tcattcatcg cagcgtacaa acctggagct taacgctggt atttttaagc
    19081 agtctggtgc tggtttttat cgagatctgg tgtgcgtttt cactggtgaa agggcgtcgc
    19141 tgggcgcgct ggctatatct gctgacacaa atcaccgccg caagttactt gtgggcggct
    19201 tcgctggggt atggttatcc ggagctgttc agcattcccg gtgaatcaaa acgtgaaatc
    19261 ttccatagcc tgatgctgca gaagctgccg gatatgctca tcctgatgct gctgttcgtt
    19321 ccctcgacca gtcggcggtt cttccagttg caataatgtg tataatcgtc gcccctgatg
    19381 atgtgaaggt caatgtatgc agtgcgcact ttacgacgcg ggtcgctgtc gttcctgtca
    19441 gtggataacg cagccgattc cagagcaact ctccgctaaa accgccgatc ttaaaaatct
    19501 gctcgccgat tttccggttg aggaatggtg cgcgccggtg tcaggcccgg aacaagggtt
    19561 tcgtaataaa gccaaaatgg tggtgagtgg tagcgttgaa aaaccactgc tcggtatgct
    19621 gcatcgcgat ggcacgccgg aagacctttg tgactgcccg ctttatcctg cctcttttgc
    19681 gcccgttttt gcggcgctaa aaccgtttat cgcccgtgcg gggttaacgc cctacaacgt
    19741 ggcgcgtaag cgtggcgaac tgaaatacat tctgctgact gaaagccaga gcgatggcgg
    19801 catgatgctg cgctttgtac tgcgttctga aaccaaactg gcacaactgc gtaaggcgct
    19861 gccgtggtta caggaacaac taccgcaact gaaagttatt accgtcaata ttcagccggt
    19921 acatatggcg attatggaag gggagacgga gatctacctg actgaacaac aggcgctggc
    19981 agagcgtttt aatgacgtac cgctgtggat ccgtccgcaa agtttcttcc agactaatcc
    20041 ggcggtcgcc agccagctgt acgctaccgc gcgcgactgg gtacggcagc tgccagttaa
    20101 acatatgtgg gatctgttct gcggtgtggg gggctttggt ttacactgcg cgacgcctga
    20161 catgcagtta accgggatcg aaattgcgcc agaggccatt gcctgtgcaa agcagtcagc
    20221 cgctgaactg ggcttaactc gtttacaatt tcaggcgctg gactccactc agtttgccac
    20281 tgctcagggg gaggtgccgg agctggtgct ggttaacccg ccgcgccgcg gcattggtaa
    20341 accgctgtgt gattatctct caacgatggc accgcgtttt atcatctact ccagctgtaa
    20401 cgcccaaact atggcgaaag atatccgcga actgcctggg tttcgcatcg aacgggtaca
    20461 gctttttgat atgttcccgc acaccgcgca ctatgaagtg ctgacgctgc tggtgaagca
    20521 ataaaaaagc cgcaggtgcg gcttcagatt gctgatcagg tgcgctttgt tcatgccgga
    20581 tgcggcgtgt acgccttatc cggcctacaa aatcatgcta aatcaataca ttgcagagtt
    20641 gatgtgggcc agataagcgc agcgcatcag gcaatcttac ttttgtcatc agtctcaatt
    20701 actgcgggaa ccactggtca ttgattttct gatatgtacc gtcagcttta attgctgcca
    20761 gcgcgttatt cagtttttcc agcagagctt tgttatccgg acgtacagcg atgcccagac
    20821 cggtgccgaa gtattgcgga tcggtcactt tttcagtagc aacgcccagt tgcggattgg
    20881 ttttcagcca ttcgtttacc accgctgtgt caccaaatac cccatcaata cgaccatttt
    20941 tcagatcgat aaaggcattc tgataactgt cataggagac agttttcact tcggggtgct
    21001 gatcctgaat atatttctgg tgcgtggtgc cgttttccat cccaatacgt ttgcctttca
    21061 gatcggcaaa cgttttgtag gtatcttttt tggcaatcac gacggctgag ttttcatagt
    21121 agggcgtggt aaacgatacc tgcttgctac gctcgggcgt gatatccata ccggagatta
    21181 cggcgtcata ttttctgaat ttcagtgacg ggatcaggct gtcgaacgcg tgattagtaa
    21241 aagtacattc tgcctgcatt tgtttgcaca aggcttttgc cagatcgata tcaaagccga
    21301 caatctcatt attagcacct atagattcaa agggtggata ggtggctgaa acgccaaaat
    21361 tgattttctc tgcggcagaa gcaccgaaag taaaggaagc aagtaaagcg gcaagaacta
    21421 actttttcat gatggaactc ccgtctgtca atcttatgat ttttggccgt gtctgcggca
    21481 tgggataaca atgccatcaa gtgaatttat atgcaataaa tatgattaaa taatttaaat
    21541 gaaataaaaa acgacggata acttagtgag ttgtccgtct tcattataag aatttatgca
    21601 ctatgtaggc cggataaggc gtccacgccg catccggcac aggcaccgtg ctgatgtctg
    21661 atgcgacgct ggcgcgtctt atcagaccta caaacccccc ggcgaatgta cgcagctaca
    21721 ttaattccgc cgttcgaatg ccagtgcttt gcgctcgatc agacgcatca tcagcgtcag
    21781 caggccgttg acgaccaggt aaataatccc tgccgcaccg aacaccatta catcgtaggt
    21841 gcgtccgtac aacaactggc tgtatcccat cacttccatc agcgtaatgg tgtaagccag
    21901 agaggtactt ttgaacacca gcaccacttc gttggaataa gaagagagcg agcgtttaaa
    21961 ggcatacggc agcaggatcg ccagcgtatc ttttttgctc attcccaggg cgctacagga
    22021 ctgccactga ccttctggga tcgcacgaat tgcaccgtaa aacagctgcg tggtatacgc
    22081 cgcactgttc agcgataacg caatcagcgc acataaccac ggttctgaca acaaatgcca
    22141 cagtgccgga tactcctgca aagtcggaaa ctggcccggc ccgtaataaa tcaggaagat
    22201 ctgcaccagc agcggcgtac cggtaaacag cgtgatataa ccccgcacca gccacaccag
    22261 caccggcgtt ttcagcgtca ggatgatggt aaaaatcaat gccagaatca gtgccacaat
    22321 cagcgaggca acggttagcg tcaggctggt gtgtagccct ttcatcagtt cgggtaaata
    22381 ctcaaacatt agctgggcct ccgctcaaaa cgtgtcgcgc gcaggtcaat gcgtttgaga
    22441 atgtactgac tgagcagggt gatcaccagg taaatcgccg ccgccacaat ataccaggta
    22501 aatggttctt gggtacgagt agcgatgctt tttgtttgca gcattaaatc attcacacta
    22561 atcaaactga ccagcgcggt atctttcagc agcaccagcc actggttacc gaggccaggc
    22621 agcgcatgac gccacatctg cggcatcacc agacggaaaa agatagccga tttcgacagc
    22681 cccagcgcct gaccagactc ccactgaccc accggcaccg ctttcaacgc gccccgaagc
    22741 gtttgcgagg cataggcggc atacagcagt gacagagcga tgacaccaca aaggaacggg
    22801 ctaacgtcga agttctcaat gtccatctgc actgggatct gcacgaaccc aagattgata
    22861 gtgaagccat ccgaaagcgt cagcagcagc tgcgaggagc caaaatagat aaacagcacc
    22921 accagaattt ctggcaggcc acgcagaatg gttaccagcg ctgaacctgc ccacgcgaca
    22981 ggacgccatt tttccgactc ccataccgca aagaacatcg ccagcgccag cccgacaatc
    23041 aatgcacaaa cggcaaggcc gacggtcatc ccggcggcgc ttgctaaagg aaaaaattca
    23101 ttcatcagga attacttctg gaaccatttg ttgtagatgg tttcgtaagt gccatctttc
    23161 ttcacttttt ccagcgcagt gttgagtttc tgctgcagct cagtgttgcc ctgacgtacc
    23221 gcgatgccga ggccagtgcc gaagtaatct ttatcggtca ctttgtcgcc caccgccgcc
    23281 agtttcgggt tatctttcag ccactcagtg accactgcgg tgtcaccgaa gacgccgtcg
    23341 atacgcccgt tttgcagatc cagttttgcg ttctggtagc tgtcatacgg aacggtagtg
    23401 atttccgggt gcttatccat aatgaatttc tggtgtgtcg tcccgttctg tacgccgact
    23461 gttttgcctt tcagctgatc aacactggtg tatttgcctt gctgacccac aaacagggca
    23521 gagttgtcat agtacggggt ggtaaacagc acctgctttt cacgctccgg agtgatatcc
    23581 atgcccgcca tcacggcttc tacgcgacgg aatttcaggc ttgggatcag gctgtcaaac
    23641 gcctggttag agaaagtgca ggttgcatca atctctttac acagcgcttg tgccaggtcg
    23701 acgtcaaaac caacgatctg gttgtttgca tcaatcgatt caaacggagg ataggatgct
    23761 tcggtagcaa aacgaatggt ttcggcagct gtggcggaaa gactaaaacc tgcaattaac
    23821 gcggcaatca gaactttttt cattgttgtt atcccgaatc ttagtgagag agatagtttt
    23881 taaatgcttc ggtttgcggc tcggtaaagc agctcgcgtc gccttgttct acgatatgac
    23941 cattttccat atacaccact cggctggcgg ttttacgcgc cacttcaact tcgtgggtga
    24001 cgatcacctg ggtaatattc gtttctgcca gctcacgaat gatgctgacg atttgtgccg
    24061 taatttccgg gtccagtgcg gcggtcggtt catcgaacag cagtacctgc ggttccatca
    24121 tcaacgcacg ggcaatagca acacgctgct gctgaccacc agaaagatgc agcgggtaac
    24181 gatcgctata aggtttgaga cgcagacgtt ccagcagttt ttctgcacgg gccagcgcct
    24241 gatctttact caaccccagt acacggcagg gcgcttcaat caggttttgc tgcacggtca
    24301 gatgcggcca caggttgtat tgctgaaaca ccatgccaac gttacgacgc aaatcgcgaa
    24361 tcgctttgtc agagggggtt ttggtgaaat cgaaatggtt gcctgcaatg ttgagcgtac
    24421 cggagcgcgg catctcaagc agattgagta cacgcagcag cgagctttta cccgcgccgc
    24481 tggggccaag taacaccagc gtttcgccct gtgggcaatc cagcgtgata tcgaacagcg
    24541 cctgatgcgc gccgtagaag caattaatgc cgtttaattg aatactcatt gacactcgta
    24601 tactggcagt ctgatagcta ttgaggtcga agatagtacc tttgacagaa taattatgca
    24661 atatttctgc tttaaaagtt aaaagcaaag cgcattattc aataaacata gcacaaaata
    24721 acgggggcgg tggtcggcga gcataaatgt cggcattcct cacgaaatgc cggacaattt
    24781 acggggttta ttggttgatc aaggcgttag cgattctcga tggactgacg gagcgtaccc
    24841 gccgtggcat gaacgctgcc gcctaagtag cgtacatcgt cgatcaccca acactggcct
    24901 tcctgaatca ttaacacttc atcctgccag ctctgatcac cctgttttag atccacacgc
    24961 agaggaatat ttcgcgcatc acgattcggg attgttgagg cactggcaac acgggcgctc
    25021 tctggcaagg ttgtgcgact ggagaatgga tcgttggtca gtagttcccg atggttatta
    25081 tcccgggagg catcgctaag cagtgtcgcc agtttgtcgc tcagataagg gcgcaaggcg
    25141 gtgatgtcgt tgctgcggtg caaaatgcgg tagtcataaa attgctgggc cacgttaccc
    25201 gggcctcctt caacgcaagg accactgcgt gtgccgttat ctttataagc tggagtgact
    25261 gtggtgcagg cactgaggag cagtgcgcag gggataagca ttgtcaattt gctgtagcgc
    25321 ataatgattt ccttataagc gatcgctctg aaagcgttct acgataataa tgatatcctt
    25381 tcaataatag cgtatcagtc tgataatgct tttgagatcg aaggcttagc aaacaaggag
    25441 atcgatcatg caattttcta caaccccaac tctggaaggc cagaccatcg ttgaatattg
    25501 cggtgtggtg accggcgaag cgattttagg tgccaatatt ttccgtgatt tctttgccgg
    25561 tatccgcgat atcgttggcg gacgttccgg tgcgtatgaa aaagaactgc gtaaagcacg
    25621 ggagatcgcc tttgaggaat taggctccca ggcgcgggcg ctgggggccg atgccgtcgt
    25681 cggtattgat atcgactacg aaacggtcgg gcaaaacggc agtatgctga tggttagcgt
    25741 cagcggtacg gcggtgaaaa cgcgtcgatg agaagagtct tctggctggt cgctgccgct
    25801 ctgttattgg cagggtgtgc aggcgaaaaa ggcattgtcg agaaagaggg atatcagctt
    25861 gatacccgac gccaggcgca ggcggcgtat ccgcgcatta aagtgctggt gatccactac
    25921 accgcagatg attttgatag ctcgctggcg acactgaccg ataagcaggt cagctcgcat
    25981 tatctggtcc ctgcggtacc accgcgatac aacggtaaac cgcgcatctg gcaactggtg
    26041 ccggaacaag aactggcctg gcatgcgggg attagcgcct ggcgcggggc aacgcgcctt
    26101 aacgacacct ctattggcat tgagctggaa aaccgtggct ggcaaaaatc ggccggagtg
    26161 aaatattttg ccccgtttga accggcacag attcaggcgc ttattccact ggcgaaagat
    26221 attattgccc gttatcacat caagccggaa aacgtagtgg cacatgcgga tatcgcaccg
    26281 cagcgcaaag acgatccggg gccattattt ccctggcagc aactggcgca gcaggggatt
    26341 ggtgcctggc cggatgcgca gcgggttaac ttttaccttg ccgggcgcgc gccgcacaca
    26401 ccggtagata ccgcgtcatt gctggagctt ttggcgcgct acggttatga cgttaaacct
    26461 gatatgacac cgcgcgagca gcggcgcgtg attatggcat tccagatgca tttccgcccg
    26521 acgttatata acggcgaagc ggatgcagaa actcaggcga ttgccgaagc attgctggag
    26581 aaatacgggc aggattagcg cggcagtttt ccgtggtcgc gtagccaggc ggcagttttc
    26641 tcgatacctt catccagggt gatgaccggc tgataaccta actcttcctg cgcacgcgta
    26701 atatccagcg taaagtcaaa attcaacttg gagacgccgt agtgggtcag cggcggctct
    26761 tttgctgact tgcggaaacg ctccatgctg cgggcgatca tatccagcat cgggtagggg
    26821 acggaacgaa tacgacagtc aatattcaac tcgtcgatca gcttctgcac gatgctgcac
    26881 agtgtgcgat gctcgccgtt ggtgatgttg tacacacgcc cggaaggtag cttatcgcag
    26941 gcttcctggc ttgccagcca cattgcgtgc acggcatttt catagtaggt catatcgacc
    27001 agcgcactgc cgccatgcgg taacagaata ctgccgtagt ggtgcatcat atgcgccaga
    27061 cggggaataa agactttatc gtgcggtccg aacagacttt gtgggcgcag aatagtaaag
    27121 cgcgtttgtg gattcgcctg cgaaaacata ttgatcactt cttcgctggc tgctttgctg
    27181 cgggcaaact cgttggcgaa gcggtgaggg cgaaaatctt ctttaatatc gcgatggtgg
    27241 tgataatcga agtacaggga gggggaagag atatgaataa agttacgcac accccaggcg
    27301 acagcccatt cacccaggcg gcgagtggcg cgaacgttag ccagatcgaa agcctgttgt
    27361 gtcccccagg gtgaggtaaa gctggagcag tgccacagcg tatcaatgcc cgcgagcatc
    27421 actttagctt gtgatgaaac cagctcggtc agatccgccg gaacaaactc tgcgcccatt
    27481 ttttccagca atttgcccat tgcctcgttg cgaccggtcg ctcgcacgct gatgcctttc
    27541 tggcataaaa actctaccgc gtttcgacct aagccgctgg tggcgccggt aaccagtacc
    27601 ttcatatcaa tccactgttg ttgagaaaat aacgtgcgca ttcttccgtg atttccccca
    27661 tgatgcaatg ggaaacatga aagaataacg caggttttgt cgattaatct gtgctttgtt
    27721 ctgccagtct ggcgatttgt tttgccattc cgcgaaaaat aaacagatgc gcggggatca
    27781 tcaataacca gtaaaacagc cccggcatac cgtgcggatg ccagaaagcg cggacatcga
    27841 tagtacgata gtcgccttta tcttccaggc taaaacacag tcgtcccagc cccggcgctt
    27901 tcatgccaaa taacaacgta agttgttttt ccggttcaac gacaatcact ttccagctat
    27961 ctaccgcatc gccagtctgt aaatattcgc gctccgggcg gcctttcgcc agcttatgac
    28021 cgatcgcgcg gtccatcaac gcccgtgtct gccacaaaat attgccaaag aaataacgct
    28081 ctttaccgcc gatttggttc actacctgcc ataaagcagc aaggctggcg gacgttttaa
    28141 cggtaaaccc cgcctgtttg gcaaaataac cgtactccgg tcgccagcgg gcaaaggcct
    28201 gagcgtcgta gccccagtcg ctggagttga ccagtttttc ctcctctttc aacgtgctac
    28261 gtactgcgtc atcgaaagcg atcagccgtt gtgggatgag tgcacgtagc gcggtatcat
    28321 ccgccagcag atcgtgtttc agcccctgaa tcaacgccct ggcggtggtg ggcggtacgg
    28381 aagtaatcac attgagaaac cacaccgaaa tccagcgggt ggggaggggg atggggatca
    28441 accagcggcg cttgccgctc accgccataa aatgttcaaa ctgttgctga taactgagca
    28501 cctctggtcc ggcggcttcg aagatgcggt gttcgctggc cggatgatct aacaacgcca
    28561 ccagatagtg cagcaagttt tccagcgcga tgggcgtggt gcgtgaacgt acccagcgtg
    28621 gcggcgttaa cactggcagg ttgtagacca tatcgcgcat gacttcgaac gccgctgaac
    28681 ctgcgccaac gataattccg gctcgaagtt cggtcacagg tacattcgct tcacgaagaa
    28741 tgtccgccgt agcctgacga gcacgcagat gatccgactg ctcatgtggc ggggcctgca
    28801 acgaactgag aaagattaat tgcttaactg gtacttcacg tagcgcatcg cggacgttga
    28861 gagccacctg gcgctcctga gcgataaaat cgccgccttc gcccatgctg tgcaccagaa
    28921 aatagacggt atcgatatcc tgcaacaggg ccggaaggtt atccggccag ctgagatcga
    28981 ctttatggca actgacgttt gccagttgca gctttgcaag cctgtcgaca tgacgtgccg
    29041 ccgccaggat ctgatgccct tgctggctga gtgtgcgcac cagatgctga ccaatgtagc
    29101 cactggcacc gagaactaaa atgcgttgcg gcacgtctct ctccttaacg cgccaggaat
    29161 gcgcgccagt gggcggcgac ttccgccagt tgttcgcgcg agacgtcaag atgcgtcacc
    29221 aggcggacaa tcggcgaggc gttaatcagc acgtttctca ctttcatgta ttcgcctaac
    29281 gcggcagcat tttcttcccc gacgcgaaca aacagcatat tagtgtcctg acgcatcaca
    29341 tccgcgcctg cttcacgcag ttgctccgcc atccaggcgg cgttgtcgtg atcttcctgc
    29401 aaccgtgcga cgttattttt cagggcatac atcccggcgg cagccagaat cccggactga
    29461 cgcatcccgc caccggccat tttccgccag cggatggcac gtttaatgta atcacgatta
    29521 ccgacgagta atgaaccgac aggcgtccca agacctttcg acaggcaaat ggtgaacgaa
    29581 tcacaatatt gcgtgatctc tttcagttcg cagccgtaag ccaccacggc attaaagatg
    29641 cgcgcaccgt caacatgcag cgccagattg cgctcgcggg taaattccca tgcttctttc
    29701 aggtattcac gcggcagcac tttgccgttg tgggtgtttt ccagactgag taatttggtg
    29761 cgggcgaaat ggatatcgtc gggtttgatt ttcatcgcca ctttatccag cggtagcgtg
    29821 ccgtcggcag ccgcgtcgat gggttgcggc tgaatactgc ccagcaccgc tgcgccaccg
    29881 gcttcaaaca gatagttatg cgcggcctga ccgacaatat actcttcgcc acgttcgcag
    29941 tgactgagca gagcgaccag gttggcctga gtgccggtag gcagaaaaat ggcggcttct
    30001 ttaccggaaa gctctgctgc atagtcctgc agagcattaa cggtagggtc gtctccgtaa
    30061 acgtcgtccc caaccggggc ggccatcatc gcttcgagca tggcgcggct cggtcgggta
    30121 acggtatcac tgcgtaaatc aatcatggca tgtccttatt atgacgggaa atgccaccct
    30181 ttttacctta gccagttcgt tttcgccagt tcgatcactt catcgccgcg tccgctgatg
    30241 attgcgcgca acatatacaa gctaaatcct ttggcctgtt cgagtttgat ctgcggtgga
    30301 atggctaact cttctttggc gaccaccaca tccaccagca ccggaccgtc gatggagaag
    30361 gcgcgttgca gggcttcatc aacttcagac gctttttcta cacggatacc cgtaatgccg
    30421 cacgcttcgg caatgcgggc aaagtttgtg tcgtgtagtt cggtgccgtc agtcaaatag
    30481 ccaccagctt tcatctccat cgccacaaag cccagcacgc tgttgttaaa gacgacaatt
    30541 ttcactggca gtttcatctg cactactgag aggaaatcgc ccatcaacat gctaaaaccg
    30601 ccatcgccgc acatggcgac cacctgacgt tctggctctg tcgcctgcgc acccagcgcc
    30661 tgcggcatgg cgttagccat cgaaccgtgg ttaaacgaac ctaacaggcg acgcttgccg
    30721 ttcattttta gataacgtgc cgcccacacc gttggcgtac caacgtcaca ggtgaaaata
    30781 gcgtcatcgg cggcaaaatg actaatttgc tgcgccagat attgcgggtg aatggctttc
    30841 tcgctcggtt tagctaaatc gtccagccct ttgcgggcgt cgcggtaatc ttccagcgct
    30901 ttatccagaa acttgcgatc ggctttttct tccaccaatg gaagcaatgc acgcagagtc
    30961 gacttgatat cgccgaccag tgccatatcc accttgctgt gagcgccgat gctggctggg
    31021 ttgatatcaa tctgaatgat tttggcatcg gtcgggtaga aggcgcggta gggaaattgc
    31081 gtgccgagta gcactaacgt gtcggcgttc atcatggtat ggaaacctga cgagaagccg
    31141 attaacccgg tcattccaac atcatacgga ttatcgtatt cgacatgttc tttaccgcgc
    31201 agggcatgaa caataggcgc tttaattttc ccggcaaact caactaactc tttatgcgcc
    31261 cccgcgcagc cgctgccaca catcagggcg atattgctgg aataacgcag cagttgcgcc
    31321 agtttgcgta actcttcttc ttccggcgtc acgactggtt gtggcgcatg ataccagtgc
    31381 gtggttgccc cttctggcgc aggttttaac gccacgtcgc ctggtaacac gacaaccgaa
    31441 acgccacggt taagcaccgc tttgcgcatg gcaatcgcca gtacttgtgg gatctgctcc
    31501 gggctggaaa ccagctcgca atagtgacta cattcgcgga atagctcttg tgggtgggtt
    31561 tcctggaaat agccgctgcc aatttcgctg gagggaatat gagcggcaat cgccagtacc
    31621 ggaacgtgat tgcggtggca atcaaacagg ccgttgatta agtgcaggtt gccggggccg
    31681 cacgatccgg cgcagaccgc cagttctccg ctaagttgtg cttcagcgcc agcggcaaag
    31741 gccgccactt cttcgtggcg ggtggacatc cactcgatgg tgcccatgcg attaagactg
    31801 tcactaagac cattcagaga gtcgcctgtg actccccaga tgcgtttcac ccctgccgat
    31861 tcgagtgttt tggcgatata agctgcaacc gtttgtttca tggttctcca tctcctgaat
    31921 gtgataacgg taacaagttt agttcatctg acggaggggg aagggatggg agagaaagga
    31981 ggcactaacg gttaaatagc ccgatgaaag gaatatcatc gggcataagg cgattatgcg
    32041 agaaccaaat ccccctgcgg atggcaggag caggccagta cgtaaccttc agcgatttcg
    32101 gcgtcggtca gcgtcattgt actgctcacc gtatattcac cggaaaccac ttttgtctta
    32161 cagcagccgc aaacacccgc acggcaggca gcgacaaccg gaacgttatt gctttccagc
    32221 gcctccagta gcgtggtgcc aaccggggcg taaaattctc gtgccggttg cagtttggtg
    32281 aatttcagac cgctggtcgc cgcttttgct actggggtga agaatttctc tttaaagaaa
    32341 cgcgtcacgc cgagcgcttt cacttcctgc tctacccaat ccatatacgg agccgggccg
    32401 caggtcatca cggtacgtga agctaagtca ggtacacctg ccagcagttc tcgagtgaga
    32461 cgaccagcga taaagccttc ggtaacgtta ttttctgcca ccagcgttac cggatagtta
    32521 cgccactcat cggcgaaaat aacatcctgc ggcgtatgca cgttgtagat cacccgcaca
    32581 tcggcctgtg gacggttctt cgcaagccag cgacgcatcg acataatcgg cgtgacgccg
    32641 cagcctgccg ccagcaacag gaatttatct tctgctttat cgtcgcaggt aaattccccc
    32701 atcgcgtccg aaagccagag ataatcaccg cgttttacat cgcgcgtcag ccactgggag
    32761 ccgacaccgt catcaatccg ccgcacggtc agggtgatat attcactcac gcctggcgtg
    32821 gaggaaatgg tgtaagcacg cagcgtttcc gctgagtttc gcacgctgac cagtgcatat
    32881 tgcccggcgc gatatgggta gtaatcgtgg caaatcaggg aaatcgtcca cacatccggc
    32941 gtttcttgcg taatgtgatg aacctgcatc cgccacgggc attgattcgt tggcatcgtc
    33001 atcgacaaac tccttacgcg ctcaacagtt gcttcatgtc ttcttcaaca gtggtgatag
    33061 aacgcaggcc gaatttctcg ttcagcaccg ccagcaggtc tggtgtcagg aaaccaggtg
    33121 cagtcgggcc ggtgacgata tttttcacgc ccagagaaag cagcgtcagc agaatgacga
    33181 tcgctttctg ttcaaaccag gagagcacca gcgacagcgg cagatcgttc acaccacagc
    33241 ccagtttctc tgccagagtg acagccagaa taatcgctga gtaagcatcg ttacattgac
    33301 ctgcatctac cagacgcggc agaccttcga tatcgccaaa ctcaagtttg ttaaagcgat
    33361 atttaccaca ggcgagggtc aggatcaggc agtcatccgg cacgatggtg gcgaaatcgg
    33421 tgaagtagtg gcgctcgccg cgtgcgccgt cacagccacc aagcaggaag atatgacgca
    33481 gtttttcacg gctcaccaga tcaatcagcg tatcagcagc gccaagcagc gtctggcgac
    33541 caaaacccac ggtgataagg tgcggaattt cgctgtacgg gaagcctgcc atctgttgcg
    33601 cctgggtgat aaccgcagag aaatcatcac cgtccagatg acgcacgcca ggccagccaa
    33661 caatgctgcg ggtccagata cgatcgtcat aagcgcttac ggttgggtcg atgatgcagt
    33721 tcgaggtcat cacgatgggg ccagggaaac gagcgaactc cacttgctga ttctgccagc
    33781 cgctgccgta gttaccgacc agatgcttga atttacgcag ctccgggtag ccatgcgcag
    33841 gcagcatttc gccgtgggtg tagacattaa cgcccgtgcc ttcggtctgt tccagcaggt
    33901 tgtagagatc tttgagatcg tgaccggaaa tcagaatgca tttacccgcc gtcgctttga
    33961 cgttgacctg ggttggcgtc gggtgaccgt atttaccggt ttcgcctgca tccagaatgc
    34021 tcatcacttt gaagttcatc tggccgattt ccattgaaca ctcaagaagc gcgttcatat
    34081 cggcaggcca ggtccccagc cacgccatga ttttatggta ctgggcataa atatcgttgt
    34141 cgtattgacc gagaacatgc gcgtgttcca tataggccgc cgcacctttc aggccataca
    34201 ggcacagcag acgcaggccg agaatgtttt cgccaatcgc cgctttatct ttgttagggg
    34261 taaattctgc tgcctgacgt tgcagctcgc cgagatcatc gctcaccagt tgcaggtcag
    34321 ccatcgggtt atcgacgcgc gcgttggcat ctacagccag gcattgtgct ttcagcgcct
    34381 cgcgcaaggc aatcgcttca cgagcgtagc cgacaatacg cggagaatcg aagttaacgt
    34441 tggtcagggt tgagaaaaag gcacgtggcg cgaagctgtc aacatcgtgg ttgatgatgc
    34501 cgtattcacg cgcttttacc gcccaggcag aaagcccttg cagcgccgcg atgagtaaat
    34561 cctgaaggtc agaagtttcc gccgttttac cacacatccc ctgcgcgtat gagcagccgt
    34621 ttcctgccgg agtacggata gtttgttcac attgcacaca aaacatgatc acacctttta
    34681 aagttatatt taatatacat gtttaaggtt aagacgctta acgcggggat aaaagggatt
    34741 tttcatgcaa ctttaaggga gattgattta gcgcaatttt ggcggcaggg atctaccgcc
    34801 agagaggtat tacgcagaga aaaaggcgat gaggatcggc actaacaggc taagaataaa
    34861 accgtgaaca attgccgccg ggaccatatc cagcccgcca gtacgttgaa gaacgggcag
    34921 ggtgaaatcc attgatgtgg caccgcataa gcccagtgca gtagagcggc tgcggcgaat
    34981 cagcccaggg atcaacataa tagcaatcag ttcacgggcc agatcattaa aaaacgccgc
    35041 gctcccgatt accggaccaa aagattcggt caataaaata ccggaaagag aataccagcc
    35101 gaaaccggag gccattgcca gcgcggtatt gatggggaga tcaagaataa aggcgttaat
    35161 taaaccacca attaatgaac tgacaaccac caccacggcg acaatcattc cccggcgatt
    35221 aaggacaatc tgctttaagg tcatgccatt attgcgcaac tgaataccaa cgaggaaaag
    35281 tagcaaaatt aacgtgtatt cactggcttc ggtcgcgtgt tgtaagaaag ccagtccact
    35341 cagaccaatg gcaaaaccaa tcactactac gccgcacagt tttagcgact ccagcgccat
    35401 cgcaatacgc gacgggagtt tttcttgctg atggtggttg cgccacggca ggcctcgctc
    35461 cagccacatc agggcggcaa tattacacag taaaataacg gtaatactga cggcagaata
    35521 atgcagaatc gccaacaggt tactggcgag gttatcgaga aacgccagac tgatacccat
    35581 aaaaaagaga ataaggtaaa ccatccagct taatagctga ttaataactt ttaacgcagc
    35641 ttgttggcga agcggaatga ggtaacccac aatcagggga accagaatga ttaacagccc
    35701 agaaaacatg aaaacccagt ccttgcaaag atgaagtcga aatgcgcgat gacacactac
    35761 tgaaagcgga aggacgagta aagttgcaat taaaaggaaa tgttatgcat aaggagcagt
    35821 agagtattcg ttttcattta aagatattct tgcgctttaa ttacaaactg caccgatgtt
    35881 ggtggcgtca aaatcgccga ggcgttccct gaaggccggg gcagcccaca tggatgtggg
    35941 ctgagggcgc gttttacagg gatgttacct cgcgcccgac ccggtagccg taagggataa
    36001 gtcgagggca ccgcgcagcg gcgattttgt tcgccagagc ccgggggggc agggggcggc
    36061 ggcgattggc cgccccctgc gcgctccttg cgccagtggc aatatgtggc ttagctcatg
    36121 aaaggagcgc aacaagatga tgaatcaaca tataacaaca tcttaaaaaa agcctgacat
    36181 tacgccaggc cttctgcgtt aattaatcac gcttttccag cagggtccgg taaatcagac
    36241 caccgataat gccgccgaca attggcacca cccagaagaa ccacagttgt tctaatgccc
    36301 agccgccctg gaagatagca accgcggtgc tgcgcgccgg gttaacagaa gtgttagtca
    36361 ccggaatact aattaagtga atcagggtta aggccagacc aatagcgatc ggcgcaaaac
    36421 ctgccggcgc gaatttggcg gttgcgccgt ggatcaccaa caggaaacct gcactcaata
    36481 ccagttcaac taccagcgcg gaaagcatgg aataaccgcc tggtgaatgc tcgccataac
    36541 cgttagaagc aaaaccgctg gctgccgcgt caaaacccgt tttaccactg gcaattaaat
    36601 acagcagcgc cgctgcaaca ataccgccga caacctgggc aattacgtag ccaacgactt
    36661 cttttgccgg aaaacgtccg ccagcccata aaccaatagt gaccgccggg ttaaaatgac
    36721 caccagaaat atgaccaaca gcaaaggcca tcgtcagaac ggtcagaccg aacgccaacg
    36781 ccacgccggc aaaaccaatg cctaattccg ggaagcctgc ggccagtaca gcactaccac
    36841 agccaccaaa aacaagccag aaagtaccaa aacattcagc tgctaatttt ctgaacatat
    36901 ccacctcaat taaaaattga ccctgtgaaa aatatggtcg ttttataggg tcgtcgtaaa
    36961 aaaatgacga cggaaataat gcgcggctat tttaaaaacg aaggcgagtc attcaccaga
    37021 taaataaatc cagtaaattt gatttagggc aacagcgggt tgccccatat agtcatttgt
    37081 ctgattgaca gtgtagtgca cgcaaaagat ttaatccttt aggcgtaata aaaaataata
    37141 tatcatgcta attatttgat tttgttgttt ttgcagactt atcagcaaga gggagtataa
    37201 cgcgattatt cgctcatttt tcagacattt gccatgctta aatgtgatgt catcacgtat
    37261 tagcaaggcc tttcccgtta tactgccagc gtaaaggata agtcacatat ttctggaggg
    37321 gatatgattc ttgagcgcgt tgaaattgtg ggttttcgcg gtatcaaccg tttgtcgttg
    37381 atgctggaac aaaacaacgt cctgattggg gagaacgcgt ggggtaaatc cagcttgctg
    37441 gacgccttaa ccctgctgct atcgccagaa tcagatctct accattttga gcgcgacgat
    37501 ttctggttcc cgccgggaga tatcaacggg cgagaacatc atctgcatat tattttgacc
    37561 ttccgcgaat cgctgccagg ccgacatcgg gttcgccgtt atcggccgct ggaagcgtgc
    37621 tggacgccat gcaccgatgg ctatcaccgt attttttatc gtctggaagg ggagagtgcg
    37681 gaagacggca gcgtgatgac actgcgcagt tttctcgata aagacggaca tccgattgat
    37741 gtcgaggata ttaacgatca ggcacgccat ctggtgcgtt taatgccggt gctgcgcttg
    37801 cgtgatgccc gttttatgcg ccgtattcgt aacggcacgg tgccaaatgt ccctaatgtg
    37861 gaagtcaccg cgcgccagct cgatttcctc gcccgtgagt tatcctcaca tccgcaaaat
    37921 ctctctgatg ggcagattcg tcagggactt tccgcaatgg tacagctgct tgagcattat
    37981 ttctctgagc agggggccgg acaggcgcga tatcgtttaa tgcggcggcg ggccagcaat
    38041 gagcaacgaa gctggcgcta tctggacatc atcaaccgga tgattgaccg acctggaggg
    38101 cgctcgtatc gggttatttt gctcggcctg tttgctactt tattgcaggc aaaaggcaca
    38161 ttgagactgg ataaagacgc gcgtccattg ttgctgatcg aagatccaga aacccgttta
    38221 caccccatta tgctttcagt tgcctggcat ctgttgaatc ttctgccatt gcagcgcatt
    38281 gccaccacca actcgggtga gttgctttcg ttaacgccgg tagagcatgt ttgccgactg
    38341 gtacgtgagt cctcgcgcgt tgccgcctgg cgtctggggc cgagtggctt gagtaccgaa
    38401 gatagccgac gcatatcctt tcacattcgt tttaaccgtc cgtcatcgct gtttgcacgc
    38461 tgctggttgc tggtggaagg ggaaacggaa acctgggtta tcaatgaact ggcgcgtcag
    38521 tgcggacatc attttgatgc cgaagggatc aaggtcattg agtttgccca gtccgggcta
    38581 aagccactgg ttaaatttgc ccgccgaatg gggattgaat ggcatgtact ggtcgatggc
    38641 gatgaagcag ggaagaaata tgccgctacg gtacgcagcc tgttgaataa cgatcgggaa
    38701 gccgaacgag aacatttaac ggcgttaccg gcgctggata tggaacattt tatgtatcgc
    38761 cagggatttt ccgatgtgtt ccaccgcgtg gcgcaaatcc cggaaaatgt accgatgaat
    38821 ctacgcaaaa ttatctcgaa agcgatccat cgctcttcca aacccgatct tgccattgaa
    38881 gtggcaatag aggcaggacg tcgtggtgtg gactccgtac cgacgctgct gaaaaaaatg
    38941 ttctcacgcg tgctgtggct ggcgcgcggt cgcgcggatt aaccgcgaaa catcgtggcc
    39001 atttgtggct gaatagcgtc gagcatctca tagcgccgac ggtattcagc ccgtttttta
    39061 ctggcgattt cggcaatctc ttttcgtgct atctgtgctg gaaggcggta atggcgttca
    39121 gcatcacata cgccgccaac cgattcccag aaagcgttgt aatcagcgtg gatcttgcct
    39181 tctttatcgc gataacgcag gctgcggtaa atatgcgttt cattgctgac ggcaataatc
    39241 tgctctacct gcaaacgttg ggcaaacaga caggccgctt ccatcacgag gcgtttggga
    39301 aatagcccgt ggcaggcttt cgtcgcattc tggatttcct gatgtggaat ttcccatttt
    39361 gcgccttgca gtccgccaat aaacatcgtt cttttcccct gatattcaca cagggtaaac
    39421 gtgatctctg ccagaggaat accttcgctg ttgcgaaaca ggattgtgct gtcaccttct
    39481 ttatccattg agatcatcat ggtcagctta agcgtgaact gctcgccgtt tttgccttcc
    39541 agcttcgcca gttgcagccc gggggtattc aaatataagc tgaattcttc cgccgacata
    39601 catccgcgga gtaacgcata atggtaacgt aacgcctcca gcaattgctt acggctaaga
    39661 ttcgccgcaa ggtaagggcg atgcagacgc acaggcagtc gcggctggcg cgttaacaac
    39721 acattgagat taggccagtg ggaaagttcg ttcatccact caacgcttaa acgcggcata
    39781 atcaacgagc gcagcaaaaa tttctggcga aaactacggc gatgccagaa tttacccggc
    39841 cgacactgtc cacgtgccag actaagaaaa agtgacaggc tgctgagaga ttcagatggc
    39901 gtaaaggtcc gttcagttag ctgcgacata ttcatgaaat cattggttat acatgacgtc
    39961 gatttcacca ttgcgtatct taaccaaaca tcaatagtgt gattactaac gtaaatttta
    40021 gggttttgtt gatatttcgt tgaagttaat gacccggatt ggcatatgga gtattcagaa
    40081 tatttatgaa aaagcggaaa accgtgaaga agcgttacgt tattgcgctg gtgatagtca
    40141 tcgccggact gattacgtta tggagaattc ttaacgcacc cgtgccgact tatcagacac
    40201 tgattgtgcg ccccggtgat ttacagcaaa gcgtgctggc gaccggaaag ctggacgcgc
    40261 tgcgtaaggt tgatgtgggc gcgcaggtca gcggtcagtt gaaaactctg tcggtggcga
    40321 ttggcgataa agtaaaaaaa gaccagcttt taggggttat tgatcctgaa caggctgaaa
    40381 accagattaa ggaggtcgaa gcaacgctga tggagctacg tgcgcagcgg cagcaggcgg
    40441 aagcggagct gaaactggcg cgggtgacgt attcccgtca gcaacgtctg gcacaaacgc
    40501 aggcggtttc acagcaggat ctcgacaccg ccgcgacgga gatggctgtg aaacaggcgc
    40561 aaattggcac cattgacgcg gaaatcaagc gcaatcaggc ttctctcgat acggctaaaa
    40621 ccaatctcga ttacactcgc atcgttgccc cgatggccgg ggaagtcacg caaatcacca
    40681 ctctgcaagg ccagacggtg attgccgcac aacaagcacc gaacattctg acgctggcag
    40741 atatgagcac catgctggta aaagcgcagg tttctgaagc ggatgtaatc cacctgaagc
    40801 cggggcaaaa agcttggttt acggtgcttg gcgatccact gacgcgctac gaggggcaaa
    40861 tcaaggatgt actaccgacg ccggaaaagg ttaacgacgc tattttctat tacgcccgtt
    40921 ttgaagtccc caaccccaat ggtttgctgc ggctggatat gactgcgcaa gtgcatattc
    40981 agctcaccga tgtgaaaaat gtgctgacga tccctctgtc ggcgttaggc gatccggttg
    41041 gcgataatcg ttataaagtc aaattgttgc gtaatggtga aacacgcgag cgtgaagtga
    41101 cgattggcgc acgtaacgat accgatgttg agattgtcaa agggctggaa gcgggcgatg
    41161 aagtggtgat tggtgaggcc aaaccaggag ctgcacaatg acgcctttgc tcgaattaaa
    41221 ggatattcgt cgcagctatc ctgccggtga tgagcaggtt gaggtgctga agggcatcac
    41281 cctcgatatt tatgccggtg agatggtagc gattgttggc gcttcaggtt ccggtaaatc
    41341 gaccctgatg aatattctcg gctgtctgga taaggccacc agcggcacct atcgcgtcgc
    41401 cggtcaggat gttgccacac tggacgccga tgcgctggcg caactgcgcc gcgagcattt
    41461 cggctttatt ttccagcgtt accatttact ttcgcattta accgccgagc agaacgttga
    41521 agtacccgcc gtctatgccg gtcttgagcg gaaacagcgg ctgcttcgcg cccaggagtt
    41581 actgcaacga ctggggctgg aagaccgtac agagtattat ccggcacagc tttcgggtgg
    41641 tcagcaacag cgcgtcagca tcgcgcgggc attgatgaac ggcggtcagg taattcttgc
    41701 cgatgaacca accggcgcac tggacagcca ttctggcgaa gaggtgatgg cgatcctgca
    41761 tcagctgcgc gatcgcgggc atatggtgat tatcgtcacc cacgatccgc aggtcgctgc
    41821 tcaggccgag cgggtgatcg aaattcgcga cggcgaaatt gtgcgcaatc ctcccgccat
    41881 tgaaaaagtg aatgttgctg gcgggacgga gcctgttgtc aacacggtgt ctggctggcg
    41941 gcagtttgtc agcggtttta acgaggcgct gacgatggca tggcgggcgc tggcagcgaa
    42001 taaaatgcgt actttactga ccatgctggg gattattatc ggtattgcgt cggtggtttc
    42061 cattgtcgtg gtgggtgacg ccgccaaaca aatggtgctg gcggatattc gttctattgg
    42121 tacgaatact attgatgtct atcccgggaa agattttggc gatgacgatc cgcaatatca
    42181 gcaggcgctg aagtacgacg acttaatcgc catccaaaaa caaccgtggg tcgcctcagc
    42241 cacacctgcc gtctcgcaaa acctgcgcct gcgttataac aatgttgatg ttgctgccag
    42301 tgccaatggc gtgagcggcg attattttaa tgtctatggc atgaccttca gtgaaggaaa
    42361 cacctttaat caggagcagc tgaacggtcg tgcgcaggtc gtggttctcg acagtaatac
    42421 tcgccgccag cttttccccc ataaagcaga tgtggttggc gaggtgattc tggtcggcaa
    42481 tatgcccgcc agagtcattg gtgtggcgga agaaaaacag tcgatgtttg gtagcagtaa
    42541 agtgctgcgt gtctggctac cttacagcac gatgtccggg cgagttatgg gccagtcgtg
    42601 gctgaactcc attactgtca gggtaaaaga aggatttgac agcgccgagg cggaacagca
    42661 actcacgcgt ttactttcac tgcgccacgg aaagaaggat ttctttacct ggaacatgga
    42721 cggcgtcttg aaaactgttg aaaagaccac acgtacttta caactgtttc tgacgctggt
    42781 ggcggtgatt tcgctggtgg tgggcggtat tggtgtaatg aatattatgc tggtgtcagt
    42841 gaccgagcgg acgcgggaaa ttggcattcg catggctgta ggtgcgcgag caagcgatgt
    42901 tttgcaacag ttcctgatcg aagccgtact ggtttgcttg gtcggtggcg cgttgggaat
    42961 aacgctgtca ctgttaattg ctttcacctt gcagcttttc ttacccggct gggagattgg
    43021 tttttcaccg ttggcactgc tgctggcgtt tctctgctcg acggccaccg ggattttatt
    43081 tggatggtta cccgcacgaa atgcggcacg actggatcca gtagatgctc tggtacgaga
    43141 gtaatttttg agataaaaat gccagccgat cgggctggca ttttgccttt aggatgtaca
    43201 caatgagaca gaagagctat gcgactgccg cttctacttc gacgggcaca ataacactgg
    43261 cgtgattgcc ttttggcccc tggtggacat caaactgaac ggattgtcca gcttttagcg
    43321 ttctgtaacc atccatctga atggtggaat aatgagcgaa aatatcttcg ccgccgcctt
    43381 cagggcagat gaaaccaaac cctttggcat tgttgaacca cttaacagta cccttttcca
    43441 tgcttcgaca tccttcgcaa atcttataca agtaagatgg aataaaccgg ggtcagagag
    43501 ggggctgttc aaaacctcgc caactctaga aatacaattt agagaattag ggcgagccgt
    43561 caagcgtttg acaggggcca gggggcaggt atgaatcaaa aatttgaagc agttaacgct
    43621 attgacagga atgtgacaga tgtcgctgat gccaacgata gatgatagtt atctatcatg
    43681 tggagtagat tggtcaggca aataagctct tgtcagcggc agggcgttct gccgataacc
    43741 gtaaccgaag atgataactg acaatgggta aaacgaacga ctggctggac tttgatcaac
    43801 tggcggaaga aaaagttcgc gacgcgctaa aaccgccatc tatgtataaa gtgatattag
    43861 tcaatgatga ttacactccg atggagtttg ttattgacgt gttacaaaaa ttcttttctt
    43921 atgatgtaga acgtgcaacg caattgatgc tcgctgttca ctaccagggg aaggccattt
    43981 gcggagtctt taccgccgag gttgcagaaa ccaaagtggc gatggtgaac aagtacgcga
    44041 gggagaatga gcatccattg ctgtgtacgc tagaaaaagc ctgaatgcag gcataaaaat
    44101 tgggggaggt gcctatgctc aatcaagaac tggaactcag tttaaatatg gctttcgcca
    44161 gagcgcgcga gcaccgtcat gagtttatga ccgtcgagca cttgttactg gcgctgctca
    44221 gtaacccatc tgcccgggag gcgctggaag cgtgttctgt ggatttggtt gcgctccgtc
    44281 aggaactgga agcctttatt gaacaaacca cacccgttct gcctgccagt gaagaggagc
    44341 gcgacacaca gccgacgctg agttttcagc gtgtactgca acgtgcggtc ttccatgtcc
    44401 agtcctccgg tcgcaatgag gtaaccggtg caaacgttct ggtcgctatc tttagcgaac
    44461 aggagtcgca ggcggcatat ctgttgcgta aacatgaagt cagccgtctc gatgtggtga
    44521 actttatctc tcatggcacg cgtaaagacg agccgacaca gtcttctgat cctggcagcc
    44581 agccaaacag cgaagaacaa gctggtgggg aggaacgtat ggagaatttc acgacgaacc
    44641 tgaatcagct tgcgcgcgtg ggcggaatcg acccactgat tggtcgtgag aaggagctgg
    44701 agcgtgctat tcaggttctc tgccgtcgcc gtaaaaacaa cccgctgctg gtgggggaat
    44761 ctggtgtcgg taaaaccgcg attgcggaag gtcttgcctg gcgaattgtt cagggcgatg
    44821 tgccggaagt gatggctgac tgtacgattt actctctcga tatcggttct ctgttagcgg
    44881 gcactaaata tcgcggcgac tttgaaaaac gttttaaagc gttgctcaag cagctggagc
    44941 aggacactaa cagcatcctg tttattgatg agatccacac cattatcggt gcgggtgcag
    45001 cgtctggtgg ccaggtcgat gcggctaacc tgatcaaacc gttgctctcc agcggtaaaa
    45061 ttcgcgtaat tggttcgaca acctatcagg agttcagcaa cattttcgag aaagaccgtg
    45121 ctctggcgcg tcgcttccag aaaattgata ttactgaacc gtcgatcgaa gaaactgttc
    45181 aaatcatcaa tggcctgaaa ccgaagtatg aagcgcacca cgacgtgcgt tataccgcaa
    45241 aagcggtgcg tgcagcggta gagctggcgg tgaaatacat taacgatcgt catctgccgg
    45301 ataaagccat tgacgttatc gacgaagcgg gcgctcgcgc acgcctgatg ccggtaagca
    45361 aacgcaagaa aaccgttaat gtggcggata ttgagtccgt ggtggcccgt attgcgcgca
    45421 ttccagagaa gagtgtttct cagagtgacc gcgataccct gaaaaacctc ggcgatcgcc
    45481 tgaaaatgct ggtcttcggt caggataaag ccattgaggc gctgactgaa gccattaaga
    45541 tggcgcgtgc aggtttaggt cacgaacata aaccggttgg ttcgttcctg tttgccggcc
    45601 ctaccggggt cgggaaaaca gaggtgacgg tacagctttc gaaagcgttg ggcattgagc
    45661 tgctgcgctt tgatatgtcc gagtatatgg aacgccatac cgtcagccgt ctgattggtg
    45721 cgcctccggg atacgttggt tttgatcagg gaggtttgct gactgatgcg gtcatcaagc
    45781 atccacatgc ggtgctgttg ctggacgaaa tcgagaaagc gcatccggac gtgttcaata
    45841 ttctgttgca ggtgatggac aacggtacgc tgaccgataa caacggacgc aaagcggact
    45901 tccgtaacgt ggtgctggtg atgaccacca acgccggggt acgtgaaact gagcgtaaat
    45961 ccattggtct tatccaccag gataacagca ccgatgcgat ggaagagatc aagaagatct
    46021 ttacaccgga attccgtaac cgtctcgaca acattatctg gttcgatcat ctgtcaactg
    46081 acgtgatcca tcaggtggtg gataaattca tcgtcgagtt gcaggttcag ctggatcaga
    46141 aaggtgtttc tctggaagtg agccaggaag cgcgtaactg gctggccgag aaaggttacg
    46201 accgggcaat gggcgcacgt ccgatggcgc gtgtcatcca ggacaacctg aaaaaaccgc
    46261 tcgccaacga actgctgttt ggttcgctgg tggacggcgg tcaggtcacc gtcgcgctgg
    46321 ataaagagaa aaatgagctg acttacggat tccagagtgc acaaaagcac aaggcggaag
    46381 cagcgcatta atctgattgt taggtaggtt ggtcaagtcc gtaatctcga aagaggttac
    46441 ggactttttg tttatggggt ggaggaggtt cagacccttt ttttaatgat gatggtaagt
    46501 tgttgataat tagtgctgcg ggaaggtaag gataaaaaag ggtgctgcag gagaatggga
    46561 tggttttgct ttatttacaa cgggctaaac gtgtagtatt tgagttcact gccgtacagg
    46621 cagcttagaa attcacaggt aacatactcc acccgcccac catgttcact gccgtacaga
    46681 cagataaaat gcgaaaaaaa agctcgcact ttcgtacgag ctcttcttta aatatggcgg
    46741 tgaggggggg attcgaaccc ccgatacgtt gccgtataca cactttccag gcgtgctcct
    46801 tcagccactc ggacacctca ccaaattgtt ttgctaccaa acctcatggg tggcaacggg
    46861 gcgctactat agggagttgg agtaaaacgg tcaagaagaa ttttaatgat aattattgtt
    46921 tgctcatact gtaaacaagt tgtgcagtat atctacatcg agacaagtta cggacttata
    46981 cttccaaagt acttcataca tatcacaaaa taaaaaggcc ggttaaaccg accttttact
    47041 cgttctttct cttcgcccat caggcggtaa aacaatcagc gactacggaa gacaatgcgg
    47101 cctttgctca ggtcgtacgg ggtcagttca acagtcactt tgtcgcccgt caggatgcgg
    47161 atgtagtttt tgcgcatttt accggagatg tgtgcagtaa ccacgtgacc gttttctaac
    47221 tctacgcgga acatggtatt aggcaacgtt tcaagaacgg taccttgcat ttcaatattg
    47281 tcttctttgg ccatctaatc ctctggggta tcactaccgt aatttgaacc ggcaagataa
    47341 tgccgaagtt ctgtaaataa gtaaagattt gcgcgctaaa tcgcaacaaa caggttcggc
    47401 acattactcc gaaaacacac ggctaagccg caccaaaagc gcaacgtata agggagcggt
    47461 gagataaacg atgggcgtta cctgacgcga aaaattcctt atcggcagcg gggtaatgag
    47521 cgtaaccaac tctgcgaccg caattataac actctgggga gaaatgtgcc gaaaacattc
    47581 attcttgtgg tgaaaacaag catcgtggta cccagaaatt attcggcaat cgtccgaggc
    47641 gcatttgatt gagataatta aggtaatccc ggcggggaat ttcgcaggca ccaagcgatg
    47701 ctgtgtgatc gttaaggacc tggcagtcga taagcttacc gccatgaccg ataaattcct
    47761 cacagaatac cagaagcgcc gttttagacg cattttccat ccggctgaac atggactcgc
    47821 cacaaaatag cgttccctgg gccacgccgt acataccgcc gacaagctca tcttcacgcc
    47881 agacttcaat ggagtgggca tggccgagtt cgtgaaggcg atggtaggct tcgaccacgc
    47941 cacgcgtgat ccaggttcct tcttcgcgat cgctggcaca gccttcaatg acctgaccaa
    48001 aagcgtaatt catcgtgaca cgatagggcg agcgtttatg aaatcgcttc atactacggc
    48061 tgatatgcag tgattctggc catagcaccg cgcggggatc gggcgaccac cagaggatgg
    48121 ggtcgcctgg agaaaaccac ggaaaaatac cacgctggta agccattaac aggcgcgcag
    48181 ggctaagatc gcccccaagt gccagcaggc cgttaggctc acgtaatgcg ccttccgggg
    48241 aagggaaggc tattgaatgg cgagaaagct gaaccaggcg catgaccgca aaactccacg
    48301 caagtcggat cgttcaataa tagcttacaa accctgcttg aactggtaat aacgcccctg
    48361 tctggcaagc agctctgcgt gagtaccttg ctcaataatt tgcccgttgt ccatcactat
    48421 tatttgttgg aaacgagaga gtccgcgaag tcgatgggtg accattaaca ccgttttctc
    48481 acgcatcatt tctgcaagca attcaaggat ctggctttcg gttgtggcat ctaagccttc
    48541 ggtaggttca tccagcaaca ccagtggcgc atcatgtaac agcgcacggg cgatagccag
    48601 acggcgcagt tcaccaccgg agagctggcg tccgccttca cctaaccaac tgttgagacc
    48661 tgcatcctcg agcagcttct ccagaccaac gcgacgcaag ctctccgcca gagcctcatc
    48721 actactgcca ggcgaggcga gtaacagatt atcacgcagc gtggcgctaa acagatgcac
    48781 tcgctgagga acaacgctga tggtctgtcg tagagccgct tcattcaggc tggctatggg
    48841 gctatcgtta agcaaaatct cgccctgttg cgggtcccaa gcgcgggtca gcagttgtaa
    48901 cagtgttgat ttgccgcatc cggttcgccc gagaatcgca atatgttccc cggcgtttac
    48961 ctgaagagaa atccctttaa gtgcctgttg ggattgctcc ggataagtga actgaacatc
    49021 ccgtaatgtc agcgaaacgc gatcggcaac acgagtttgg gtatcaggga aggtgacttc
    49081 cggtttttga tccgttaaat cagtgatccg aacggcagag gcaatgactt gccccagatg
    49141 ctgaaatgca cccgttactg gtgccagtgc ttcaaacgcg gctaacgcgc agaagacaaa
    49201 cagggcaatt aacgcgccgg gttgagcatt gccgccaacg ccgccagacg ccatccacag
    49261 catcaggatc accgctaacg cgccaatgag cagcattatc gcttgcgaca atgcggtcag
    49321 ttcagattga cggcgttgcg cttccagcca ttgaatttct gtattctcta gttgcgtgcg
    49381 ataacgatcg ctggcaccaa aaatggtcag ctcagcttgc ccttgcagcc aggccgtcag
    49441 ttgttggcga tactgtccgc gaagatgagt cagattttgc ccggtgcttt ttcccgcacg
    49501 ataaaacagc ggtggcatca ggaaaagcgt cagtaacata atgccgccca gcgtaaaggc
    49561 gagggtgaaa tcaaggaaac ttaacccgat tgtcaccatc ataatcacca caaaagcgcc
    49621 caccagcggc gagataacgc gcaggtaaag atgatcgagc gtatcaacat ccgccaccac
    49681 gcgattgagc aattcgccct gacgatagcg cgccagtccg gcaggggaga ggggcaacaa
    49741 tttgctgaag gtgtaaatgc gcagatgctg caacacgcgg aaagtcgcgt cgtgacttac
    49801 cagacgttca aaataacgcc cggcagtacg ggtgattgcg gcaccacgca cgcccgcagc
    49861 gggtagcata tagttgaagc tgtacagtcc ggcaaccccc gcaaccgctg aggccgagag
    49921 gaaccagccg gaaagtgtca acagtccgat actggcgagc agcgtcacaa ttgccagcac
    49981 aataccaaga cttaacatcc atttatgacg tttatacagt gctagatagg gtagcaaagc
    50041 gcgcatttaa atctcctcct gacgatgggc cagtaatgtg gcgaatgggc caccagccac
    50101 acttaattcc gcgtaacgtc cttgctcaat aatccggcca tcctgcataa cccaaatgac
    50161 atcccagtca gcaagatctt ctaactggtg ggtgaccatt aacgttgtct ggcgcagaga
    50221 ggcggcattc agcgcctcca ttacgcgctg ttcactgtga gcatcaaggc tggcagcggg
    50281 ttcatccaac agtaatagcg aacagggatt tagtaacgca cgggccaccg ccacgcgctg
    50341 cgcctgcccc acggaaaggc gggcagcctg gtcgccaaca ggcgtatcaa cgccttgtgg
    50401 caggagcggt agaaactcgc tgacccaggc gttatccagc gctgcttgta attcatgttc
    50461 gctggcatca ggtcgcgcca gtagtacgtt atcccgtaat gttgctgccg gtaattgtgg
    50521 attttgccca acccaggaga gatgtttacg ccatgattct ggtgataaat cgcgtaattc
    50581 tatcccgttg attcgtagcg atccctgata tgagagaaaa ccagaaagcg cgttcaacag
    50641 tgaactttta cctgaaccgc tgcgaccaac caacacggca cgttggcctg ctggcaaagt
    50701 aaagttcagc ggtccggcca gcgttttacc ttccggcgac gtgataaaca gatcctcggc
    50761 ctcaatggtc accggatcgg tcgatgctaa ttccacctcg ccacactgcg gatgggcgag
    50821 cggggtttcc ataaacgttt tcagactgtc agctgcacca acagcctggg ctttagcatg
    50881 ataaaacgta ccgagatcgc gtaatggctg gaaaaactct ggcgcaagga tcagggccag
    50941 aaaacccgca gccagcgtca cgccagtatc gtagtgacca aaatccagct cgccaagata
    51001 ggaaaaacca aagtagaccg ccaccagagc aattgacagc gaggtaaaaa attcgagaat
    51061 gccggaggat aaaaatgcca gccgtagcac ttccattgtc cgttggcgga aatcttccga
    51121 agcagaacga atactttcaa tttcagcttc accacgacca aaaatacgca atgtttccat
    51181 gccgcgcagg cgatcgagga aatgcccact taagcgagca agagcgagaa agttacgtcg
    51241 gttagcatcg gcagccccca ttccaaccag cgccataaac aacggaatta acggtgcagt
    51301 gcccagcaga atgagcgccg cagcccagtt tgaggggaag attgccacca caatcagcaa
    51361 cggcaccgac actgccagcg ccatttgcgg caggtagcgt gcatagtaat catgcatatc
    51421 gtcaatttgc tcgagtacca gcgtcgccca gctccccgca ggtttaccct gaatccacgc
    51481 tggccctgct tgttgcagac ggtcgagaac ctgccggcgg atggcaaagc ggatatgctg
    51541 cccggcgtga taacccaccc gttcgcgtaa ccagaccacc catgcgcgca gtacaaaggt
    51601 cagaaccagt aacgtaaagg gaagcagcag ggcttcacgg ggaatattct ccataatcat
    51661 atgttgcaga attcgcgcca tgaaccaggc ctgggcaatg atcaatatgc cgctcacaaa
    51721 gcccagcaga cgagaaatat tcagccaacg ttgggagatg acgctttgct gttttaacca
    51781 gcgggttaac tctttttgac gagatttatt cattgcacgc ttagcaggtg agttatcaga
    51841 attatttgca gagcaatgtt acaacgggga aaaaataaag gcgacccata gtcgcaaggt
    51901 gtcgccttct ttacttttgt tactgatttg taaaattatt ttgcgtcagc taaaccatcg
    51961 aggtagcgtt ccgcatcaag tgctgccatg cagcctgtac cggccgaagt aatggcctga
    52021 cgataaatat gatccatcac gtcacccgcg gcaaagacgc caggaatgct ggtctgggtg
    52081 gcattaccat gaatacccga ctgtactttg atgtagccgt tttccagttc cagctgccct
    52141 tcgaaaatgg cagtattcgg gctgtgaccg atagcaacaa acagaccggc aacgtcgagt
    52201 gattcgatgt tatcgctgtt ttgcgtatcg cgcagacgaa cgccagtgac acccatttga
    52261 tcgccggtca cttcttccag cgtccggttg gtgtgcagaa tgatgttgcc gttctccact
    52321 ttatccatca gacgcttaat gaggattttt tccgcgcgga aaccgtcacg gcggtgaatc
    52381 agatgtactt ccgaagcgat gttagacaga tacaacgcct cttcaaccgc ggtattgccg
    52441 ccgccgatga ccgcaacttt ctggttgcga tagaagaaac cgtcgcaggt tgcacaagca
    52501 gaaaccccac ggcctttaaa ggcttcttca gagggcaggc cgagatagcg tgcagaagct
    52561 ccggtggcaa taatcagcgc gtcgcaagtg tattcgccgt tatcgccatt cagacggaac
    52621 ggacggtttt gcagatccac cttgttgata tgatcaaaaa tgatttcagt ttcaaatttg
    52681 gtggcatgtt cgtgcatgcg ctccattaat aacggaccgg tcagatcgtt tggatcgcca
    52741 ggccagtttt ccacttctgt ggtggtggtc agttggccgc ctttttccat gccggtaatc
    52801 agcacaggtt gcaggttggc gcgcgccgca tagacagcag cggtgtatcc cgccgggcct
    52861 gaacccagga taagcagttt actgtgtttg gtcgtgccca tgagatcccc atagttgttg
    52921 gcagacaatg ggcaggattg tagggaattt actgacgtaa aaaaagagta tgacgatttt
    52981 gttaacaatt tgtgcaatcg gcagcatcga taagcaggtc aaattctccc gtcattatca
    53041 cctctgctac ttaaatttcc cgctttataa gctgattaaa tgatgaataa acgcccctgt
    53101 taatgaatat ctggcatgtt gtactaaaaa tcgatgtttt gctttgacaa tcccctggtg
    53161 ttttgcgaaa acattcgagg aagaaaaaaa acagtattct tatatgcgca taaccatgca
    53221 tgtaaatacc atgtttaccg tgctagtgaa atctacgtat ggcgtggaca gacgccattc
    53281 gtgatgtcga tagctgccac aaggcaacgg tcttctcacc gtagacccag gcattgcgcg
    53341 ccgtgaatct tcatgatttc ggtctatcgt gacgggtagc gactctgaac agtgatgttt
    53401 cagggtcaga caggagtagg gaaggaatac agagagacaa taataatggt agatagcaag
    53461 aagcgccctg gcaaagatct cgaccgtatc gatcgtaaca ttcttaatga gttgcaaaag
    53521 gatgggcgta tttctaacgt cgagctttct aaacgtgtgg gactttcccc aacgccgtgc
    53581 cttgagcgtg tgcgtcggct ggaaagacaa gggtttattc agggctatac ggcgctgctt
    53641 aacccccatt atctggatgc atcacttctg gtattcgttg agattactct gaatcgtggc
    53701 gcaccggatg tgtttgaaca attcaatacc gctgtacaaa aacttgaaga aattcaggag
    53761 tgtcatttag tatctggtga tttcgactac ctgttgaaaa cacgcgtgcc ggatatgtca
    53821 gcttaccgta agttgctggg ggaaaccctg ctgcgtctgc ctggcgtcaa tgacacacgg
    53881 acatacgttg ttatggaaga agtcaagcag agtaatcgtc tggttattaa gacgcgctaa
    53941 cacggaacag gtgcaaaatc ggcgtatttt gattacactc ctgttaatcc atacagcaac
    54001 agtactgggg taacctggta ctgttgtccg ttttagcatc gggcaggaaa agcctgtaac
    54061 ctggagagcc tttcttgagc caggaataca ctgaagacaa agaagtcaca ttgacaaagt
    54121 taagtagcgg ccgtcgcctt ctggaagcgt tgctgatcct tattgtcctg tttgccgtct
    54181 ggttgatggc tgccttacta agctttaacc cttcggaccc cagctggtcg caaacggcct
    54241 ggcatgaacc tatccataat ttaggtggga tgcccggtgc gtggctggca gatacgctgt
    54301 tctttatttt tggcgtgatg gcttacacca ttcccgtcat tattgtcggc ggttgttggt
    54361 ttgcctggcg tcatcagtcc agtgacgaat acattgatta ttttgccgtt tcgttacgca
    54421 tcattggcgt tttggcgctc atccttacct cctgtggtct ggcggcaatc aacgctgacg
    54481 atatctggta ttttgcctcc ggtggcgtca ttggcagctt actaagcact acgttacaac
    54541 cactgctaca cagtagcggg ggaactattg cgctgctctg cgtttgggct gcgggcctga
    54601 cgctgttcac cggttggtca tgggtgacca ttgctgaaaa actcggcggc tggattttaa
    54661 acattctcac cttcgccagt aatcgtaccc gtcgcgatga tacctgggtc gatgaagatg
    54721 aatatgaaga tgaaaaccac ggcaaacagc atgaatcacg ccgtgcccgt attcttcgcg
    54781 gcgcgctagc gcgtcgtaaa cggttggcgg aaaaattcat taatccgatg gggcggcaaa
    54841 cagacgctgc gttgttctcc ggtaagcgga tggatgatga agaagagatt atctacactg
    54901 cacgcggtgt ggctgccgac ccggacgacg tcctattttc gggcaatcgt gcaacgcagc
    54961 cagaatatga cgaatacgat ccattattaa acggtgcgcc aattaccgaa cctgttgctg
    55021 tagcagctgc tgctaccacg gcgacacaaa gctgggctgc gccggttgaa cctgtgaccc
    55081 agacgccgcc tgttacttct gttgatgttc caccttcgca acctacagta gcctggcagc
    55141 ctgtaccggg tccacaaacg ggagagccgg ttattgctcc tgcaccggaa ggttacccac
    55201 agcagccaca atatgcgcag cctgcagtgc aatataatga gccgctgcaa caacctgtac
    55261 agccacagca gccgtattat gcgcctgcag ctgaacaacc tgtgcaacaa ccttactacg
    55321 ctcctgcggc ggaacaacct gttcagcaac cgtattacgc aactgcgccg gaacagcctg
    55381 cgcaacagcc gtattatgcc cctgcgccag aacaaccggt ggcaggtaac gcctggcaag
    55441 ccgaagagca gcaatccact tttgctccac agtctacata ccagactgag caaacttatc
    55501 agcagccagc cgctcaggag ccgttgtacc aacagccgca acccgttgaa cagcagcctg
    55561 ttgtggagcc tgaacccgtt gtagaagaga caaaacccgc gcgtccgccg ctttactatt
    55621 ttgaagaagt ggaagagaag cgagcccgtg aacgtgaaca acttgcggcc tggtatcaac
    55681 cgattccaga accggttaaa gaaccagaac cgatcaaatc ttcgttgaaa gcgccttctg
    55741 ctgcagcagt acctccagta gaagccgctg ccgctgtttc tccgctggca tctggcgtga
    55801 aaaaagcgac actggcgacg ggggctgccg caaccgttgc cgcgccagtc ttcagtctgg
    55861 caaatagcgg tggaccgcgt ccgcaagtca aagagggaat tggcccgcag ttgccacgac
    55921 cgaaacgtat tcgcgtgcca actcgtcgtg aactggcgtc ttacggtatt aagctgccct
    55981 cacagcgtgc ggcggaagag aaagcccgtg aagcccggcg caatcagtat gattctggcg
    56041 atcagtataa cgatgatgaa atcgatgcga tgcagcagga tgaactggca cgtcagttcg
    56101 cccagacaca gcagcaacgc tatggcgaac agtatcaaca tgatgtgccc gtaaacgcag
    56161 aagatgcaga tgctgcggca gaggctgaac tggctcgtca gtttgcgcaa actcaacaac
    56221 aacgttattc cggcgaacaa ccggctgggg cgaatccgtt ctcgctggat gattttgaat
    56281 tttcgccaat gaaagcgttg ctggatgatg gtccacacga accgttgttt acgccaattg
    56341 ttgaacctgt acagcagccg caacaactgg ttgcaccgca gcagcagtat cagcagccac
    56401 aacagccggt tgcgccacag cagcaatatc agcagccgca acagccggtt gcgccgcagc
    56461 cgcagtatca gcagccacaa cagcaggttg cgccgcagcc acaatatcag cagccgcaac
    56521 aaccggttgc accgcagcag caggataccc tgcttcatcc gctgttgatg cgtaatggcg
    56581 acagccgtcc gttgcataaa ccgacgacgc cgctgccttc tctggatttg ctgacaccgc
    56641 cgccgagcga agtggagccg gtagatacct ttgcgcttga acaaatggct cgcctggtgg
    56701 aagcgcgtct ggctgatttc cgtattaaag ccgatgtcgt caattactct ccggggccgg
    56761 ttatcactcg ctttgaactt aacctggctc ctggcgtaaa agcggcgcgc atttctaact
    56821 tgtcacggga ccttgcccgt tcactttcga cggtggcggt gcgtgtcgtt gaagttattc
    56881 ctggcaaacc ctatgtaggt ctggagttac caaataaaaa acgacaaacc gtttatctgc
    56941 gcgaagtttt ggataacgcc aaattccgcg ataatccgtc gccattaacc gtggtgctgg
    57001 gtaaagatat cgccggtgag ccggtggttg ccgatctggc gaaaatgccg cacttgttgg
    57061 ttgcggggac taccggttcc ggtaaatctg tcggtgtgaa cgcgatgatc ctgagcatgc
    57121 tttataaagc acagccagaa gatgtgcgtt tcatcatgat cgacccgaaa atgctggagc
    57181 tttcggttta tgaaggcatt ccgcatctgt taacggaagt cgttactgat atgaaagatg
    57241 ccgccaacgc gctgcgctgg tgtgttaacg agatggagcg tcgttataaa ctgatgtctg
    57301 cgctgggtgt gcgtaatctg gcgggttata acgaaaaaat tgctgaagcc gatcgcatga
    57361 tgcgtccgat tccagacccg tactggaagc cgggtgacag tatggatgcc cagcatccgg
    57421 tgctgaaaaa agaaccatac attgtggtgt tggttgacga atttgccgac ctgatgatga
    57481 cggtaggtaa aaaagtggaa gagctgatag cacgtctggc gcaaaaagcc cgtgccgcgg
    57541 gtatccacct cgtactggca actcagcgtc catcggttga tgttattact ggtctgatta
    57601 aagcgaatat tccgacccgt atcgccttta ccgtatccag taagattgac tcacgtacca
    57661 ttcttgatca ggctggcgcg gaatcactgc tggggatggg ggatatgctc tactctgggc
    57721 cgaactccac gttgccggta cgtgtccatg gtgcttttgt tcgcgatcag gaagttcatg
    57781 ccgtggtgca ggactggaaa gcgcgtggtc gcccacagta tgttgatggc atcacttccg
    57841 acagtgaaag cgaaggtggc gcgggcggtt tcgatggcgc tgaagaactg gatccgttgt
    57901 tcgatcaggc ggtgcagttt gtcactgaaa aacgcaaagc gtcaatttct ggcgtacagc
    57961 gtcagttccg cattggttat aaccgtgcag cgcgtattat cgaacagatg gaagcgcagg
    58021 ggattgtcag cgaacagggg cacaacggta atcgtgaagt gctggcccca ccgccgtttg
    58081 actaattaat gcattgccgg ataaggcgcg gtagcgtcgc atccggcact ctatcaactg
    58141 aaaattcagt attttctttt ttcctcaagc tgattattag cctggaatag agagtagagg
    58201 gaactcccga tcgggagtga cgtaatttga ggaataatga tgaaaaaaat tgccatcacc
    58261 tgtgcattac tctcaagctt agtagcaagc agcgtttggg ctgatgccgc aagcgatctg
    58321 aaaagccgcc tggataaagt cagcagcttc cacgccagct tcacacaaaa agtgactgac
    58381 ggtagcggtg cggcggtgca ggaaggtcag ggcgatctgt gggtgaaacg tccaaactta
    58441 ttcaactggc atatgacaca acctgatgaa agcattctgg tttctgacgg taaaacactg
    58501 tggttctata acccgttcgt tgagcaagct acggcaactt ggctgaaaga tgccaccggt
    58561 aatacgccgt ttatgctgat tgcccgcaac cagtccagcg actggcagca gtacaatatc
    58621 aaacagaatg gcgatgactt tgtcctgacg ccgaaagcca gcaatggcaa tctgaagcag
    58681 ttcaccatta acgtgggacg tgatggcaca atccatcagt ttagcgcggt ggagcaggac
    58741 gatcagcgca gcagttatca actgaaatcc cagcaaaatg gggctgtgga tgcagcgaaa
    58801 tttaccttca ccccgccgca aggcgtcacg gtagatgatc aacgtaagta gaggcacctg
    58861 agtgagcaat ctgtcgctcg atttttcgga taatactttt caacctctgg ccgcgcgtat
    58921 gcggccagaa aatttagcac agtatatcgg ccagcaacat ttgctggctg cggggaagcc
    58981 gttgccgcgc gctatcgaag ccgggcatct gcattctatg atcctctggg ggccaccggg
    59041 taccggcaaa acaactctcg ctgaagtgat tgcccgctat gcgaacgctg atgtggaacg
    59101 tatttctgcc gtcacctctg gcgtgaaaga gattcgcgag gcgatcgagc gcgcccggca
    59161 aaaccgcaat gcaggtcgcc gcactattct ttttgttgac gaagttcacc gtttcaacaa
    59221 aagccagcag gatgcatttc tgccacatat tgaagacggc accatcactt ttattggcgc
    59281 aaccactgaa aacccgtcgt ttgagcttaa ttcggcactg ctttcccgtg cccgtgtcta
    59341 tctgttgaaa tccctgagta cagaggatat tgagcaagta ctaactcagg cgatggaaga
    59401 caaaacccgt ggctatggtg gtcaggatat tgttctgcca gatgaaacac gacgcgccat
    59461 tgctgaactg gtgaatggcg acgcgcgccg ggcgttaaat acgctggaaa tgatggcgga
    59521 tatggccgaa gtcgatgata gcggtaagcg ggtcctgaag cctgaattac tgaccgaaat
    59581 cgcaggtgaa cgtagtgccc gctttgataa caaaggtgat cgcttttacg atctcatttc
    59641 cgcactgcat aaatcggtac gtggtagcgc acccgatgcg gcgctgtact ggtatgcgcg
    59701 aattattact gccggtggcg atccgttata tgttgcgcgt cgctgtctgg cgattgcgtc
    59761 tgaagatgtg ggtaatgctg atccacgagc gatgcaggtg gcaattgcag cctgggattg
    59821 ctttactcgc gttggcccgg cggaaggtga acgcgccatt gctcaggcga ttgtttacct
    59881 ggcctgcgcg ccaaaaagca acgctgtcta cactgcgttt aaagctgcgc tggcggatgc
    59941 tcgcgaacgt ccggattatg acgtgccggt tcatttgcgt aatgcgccga cgaaattaat
    60001 gaaggaaatg ggctacgggc aggaatatcg ttacgctcat gatgaagcaa acgcttatgc
    60061 tgccggtgag gtttacttcc cgcgggaaat agcacaaaca cgctattatt tcccgacaaa
    60121 caggggcctt gaaggcaaga ttggcgaaaa gctcgcctgg ctggctgaac aggatcaaaa
    60181 tagccccata aaacgctacc gttaatgtta tcgttgcggt aatgttgtta ctgtatccct
    60241 gtggtcgcag gctgtggcca catctcccat ttaattcgat aagcacagga taagcatgct
    60301 cgatcccaat ctgctgcgta atgagccaga cgcagtcgct gaaaaactgg cacgccgggg
    60361 ctttaagctg gatgtagata agctgggcgc tctcgaagag cgtcgtaaag tattgcaggt
    60421 caaaacggaa aacctgcaag cggagcgtaa ctcccgatcg aaatccattg gccaggcgaa
    60481 agcgcgcggg gaagatatcg agcctttacg tctggaagtg aacaaactgg gcgaagagct
    60541 ggatgcagca aaagccgagc tggatgcttt acaggctgaa attcgcgata tcgcgctgac
    60601 catccctaac ctgcctgcag atgaagtgcc ggtaggtaaa gacgaaaatg acaacgttga
    60661 agtcagccgc tggggtaccc cgcgtgagtt tgactttgaa gttcgtgacc acgtgacgct
    60721 gggtgaaatg cactctggcc tcgactttgc agctgcagtt aagctgactg gttcccgctt
    60781 tgtggtaatg aaagggcaga ttgctcgcat gcaccgcgca ctgtcgcagt ttatgctgga
    60841 tctgcatacc gaacagcatg gctacagtga gaactatgtt ccgtacctgg ttaaccagga
    60901 cacgctgtac ggtacgggtc agctgccgaa atttgctggc gatctgttcc atactcgtcc
    60961 gctggaagaa gaagcagaca ccagtaacta tgcgctgatc ccaacggcag aagttccgct
    61021 gactaacctg gtgcgcggtg aaatcatcga tgaagatgat ctgccaatta agatgaccgc
    61081 ccacacccca tgcttccgtt ctgaagccgg ttcatatggt cgtgacaccc gtggtctgat
    61141 ccgtatgcac cagttcgaca aagttgaaat ggtgcagatc gtgcgcccgg aagactcaat
    61201 ggcggcgctg gaagagatga ctggtcatgc agaaaaagtc ctgcagctgc tgggcctgcc
    61261 gtaccgtaaa atcatccttt gtaccggcga catgggcttt ggcgcttgca aaacctatga
    61321 cctggaagta tggatcccgg cacagaacac ctaccgtgag atctcttcct gctccaacgt
    61381 ttgggatttc caggcacgtc gtatgcaggc acgttgccgc agcaagtcgg acaagaaaac
    61441 ccgtctggtt cataccctga acggttctgg tctggctgtt ggtcgtacgc tggttgcagt
    61501 aatggaaaac tatcagcagg ctgatggtcg tattgaagta ccagaagttc tgcgtccgta
    61561 tatgaacgga ctggaatata ttggctaata cccaattttt ctgaatctaa aaagcgcctg
    61621 cgggcgcttt ttttgtctcc ctttgatacc gaacaataat tactcctcac ttacacgtaa
    61681 tactactttc gagtgaaaat ctacctatct ctttgatttt caaattattc tatgtataca
    61741 agcctatata gcgagctgct atagaaataa ttacacaata cggtttgtta ctggaatcaa
    61801 tcgtgagcaa gcttgagtga gccattatga aaacgaaaat ccctgatgcg gtattggctg
    61861 ctgaggtgag tcgccgtggt ttggtaaaaa cgacagcgat cggcggcctg gcaatggcca
    61921 gcagcgcatt aacattacct tttagtcgga ttgcgcacgc tgtcgatagc gccattccaa
    61981 caaaatcaga cgaaaaggtt atctggagcg cctgtacagt taactgtggt agtcgctgcc
    62041 cgctacgtat gcacgtcgtg gacggtgaaa tcaaatatgt cgaaacggac aataccggcg
    62101 atgacaatta cgacggcctg cactaggttc gcgcctgcct gcgtgggcgt tccatgcgtc
    62161 gccgtgtcta caatccggac cgcctgaaat atccgatgaa acgagtcggg gcgcgcggtg
    62221 aaggcaaatt cgagcgcatt agctgggaag aagcctacga catcatcgcg accaatatgc
    62281 agcgcctgat caaagagtac ggcaacgagt ctatctatct gaactatggc accggtacgc
    62341 tgggcggcac catgacccgc tcctggccgc cgggaaatac cctggtcgcg cggctgatga
    62401 actgctgcgg cggctatctg aaccattacg gcgactactc ctccgcgcaa attgcggaag
    62461 gtttgaacta tacctacggc ggctgggcag atggcaacag cccgtcggat atcgaaaaca
    62521 gtaagctggt agtgctgttt ggtaataacc ctggcgaaac gcgaatgagt ggcagtgggg
    62581 tgacttacta tcttgaacag gcacgccaga aatctaatgc ccgcatgatc atcatcgatc
    62641 cgcgctatac cgacaccggt gccgggcgcg aagatgagtg gatccctatt cgtccgggaa
    62701 cagatgccgc actggttaac ggtctggcgt acgtcatgat cactgaaaac ctggtggatc
    62761 aggcattcct cgataaatat tgcgttggct acgatgagaa aaccctgcca gccagtgcgc
    62821 cgaaaaatgg ccactataaa gcttatattc tgggtgaagg gccagatggc gtggctaaaa
    62881 caccggaatg ggcctcacaa atcaccggtg ttccggcaga caaaatcatc aaactggctc
    62941 gtgaaatcgg cagtaccaaa ccggcgttta tcagccaggg atggggcccg cagcgtcacg
    63001 ctaacggtga aatcgcaacc cgtgctatct cgatgctggc gattctgacc ggtaacgttg
    63061 gtattaacgg aggcaacagc ggcgcgcgtg aaggttcata cagcttaccg tttgtccgta
    63121 tgccgacctt ggaaaacccg atccagacca gcatttcgat gtttatgtgg accgatgcca
    63181 ttgaacgtgg cccggaaatg acggcgctgc gtgatggtgt acgcgggaaa gataagctgg
    63241 atgtgccgat caaaatgatc tggaactatg ccggtaactg cctgattaac cagcattctg
    63301 aaatcaaccg tacccatgaa atccttcagg atgataagaa gtgcgagctg attgtggtta
    63361 tcgactgcca catgacctca tcggcgaaat atgctgacat cctgctgcct gactgcaccg
    63421 cttccgaaca gatggacttt gcgctggatg catcctgcgg gaatatgtct tacgtgattt
    63481 tcaacgatca ggtgattaaa ccgcgctttg aatgtaagac catctatgaa atgaccagcg
    63541 aactggcaaa acgtcttggc gttgagcaac agtttactga aggccgtaca caggaagagt
    63601 ggatgcggca tctgtatgcc cagtcgcggg aagcgattcc tgaactacca acgtttgaag
    63661 agttccgcaa gcaggggatc tttaaaaagc gcgacccaca agggcaccac gttgcttata
    63721 aagctttccg tgaagatcct caggcaaatt cactgaccac gccatcgggc aaaattgaga
    63781 tttattcgca ggcgctggct gacattgccg ctacctggga attgccagaa ggagatgtga
    63841 tcgatccact gccgatctac acgccgggct ttgaaagtta tcaggatccg ttgaacaaac
    63901 agtatccgct gcagcttacg ggtttccact ataaatctcg cgttcactca acttacggcg
    63961 acgttgatgt gctgaaagca gcttgccgtc aggaaatgtg gatcaacccg cttgatgcgc
    64021 aaaaacgcgg tatcaacaac ggcgataaag tcaggatctt taacgatctt ggtgaggttc
    64081 atattgaggc gaaagtgacg ccacgaatga tgccgggtgt ggtcgcactg ggtgaaggtg
    64141 cctggtatga cccggataca aaacgtgtcg ataagggcgg ttgtatcaac gtactgacca
    64201 ctcaacgtcc gtctcctctc gctaagggga atccgtcaca tacaaacctt gttcaggttg
    64261 aaaaggtgta aggagtaacc gatgacaacc cagtatggat tttttattga ttccagccgt
    64321 tgcaccggtt gcaaaacctg cgagctggcc tgtaaagact acaaagattt gacgccagaa
    64381 gtcagcttcc gccgcattta tgaatatgct ggcggcgact ggcaggaaga taacggtgtc
    64441 tggcaccaga acgtgtttgc ctactatctg tcgatttcat gtaaccactg cgaagatccg
    64501 gcttgtacta aagtctgccc gagcggtgcg atgcataaac gtgaagatgg ttttgttgtg
    64561 gtcgatgaag atgtgtgcat tggctgccgc tactgccata tggcttgccc gtatggcg
//
LOCUS       DADLFA010000003        62833 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000003 DADLFA010000000
VERSION     DADLFA010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 62833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62833
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.003"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..575
                     /gene="casA"
                     /locus_tag="JQH65_000138"
     CDS             <1..575
                     /gene="casA"
                     /locus_tag="JQH65_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HAY6137666.1"
                     /translation="VGNSKNNTELPAQVVKLLNASNLKQTRVGLWGFGFDFEDMKVRC
                     WYEHHFPLLLNKKEDLIPKLRLAAQAASHILSLLHRALKEAWFSEKKTTKLDFGFVDI
                     DFWNKTQHRFLRLVRKIEEGQDPDELLSKWQKEMWLFARQDFDDRVFTNPYEPVDLER
                     VMTARKKYFTTSAEKQNANAAREKKQEAAE"
     gene            572..1140
                     /gene="casB"
                     /locus_tag="JQH65_000139"
                     /pseudo
     CDS             572..1140
                     /gene="casB"
                     /locus_tag="JQH65_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
     gene            1155..2224
                     /gene="cas7e"
                     /locus_tag="JQH65_000140"
                     /pseudo
     CDS             1155..2224
                     /gene="cas7e"
                     /locus_tag="JQH65_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
     gene            2235..2981
                     /gene="cas5e"
                     /locus_tag="JQH65_000141"
     CDS             2235..2981
                     /gene="cas5e"
                     /locus_tag="JQH65_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HAY6137667.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSLSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            2963..3613
                     /gene="cas6e"
                     /locus_tag="JQH65_000142"
     CDS             2963..3613
                     /gene="cas6e"
                     /locus_tag="JQH65_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HAY6137668.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPSGKERQ
                     FLYRREELQGAFRFFVLSQERPAESDTFTIECRSFAPELCTGQQLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGQAEGSDVWLHQQQAALDWLAAQGERSGFTLLDISVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            3610..4530
                     /gene="cas1e"
                     /locus_tag="JQH65_000143"
     CDS             3610..4530
                     /gene="cas1e"
                     /locus_tag="JQH65_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1"
                     /protein_id="HAY6137669.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLQVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPPPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRG"
     gene            4530..4823
                     /gene="cas2"
                     /locus_tag="JQH65_000144"
     CDS             4530..4823
                     /gene="cas2"
                     /locus_tag="JQH65_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2"
                     /protein_id="HAY6137670.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(5030..6067)
                     /gene="iap"
                     /locus_tag="JQH65_000145"
     CDS             complement(5030..6067)
                     /gene="iap"
                     /locus_tag="JQH65_000145"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HAY6137671.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            6319..7227
                     /gene="cysD"
                     /locus_tag="JQH65_000146"
     CDS             6319..7227
                     /gene="cysD"
                     /locus_tag="JQH65_000146"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HAY6137672.1"
                     /translation="MAQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            7229..8656
                     /gene="cysN"
                     /locus_tag="JQH65_000147"
     CDS             7229..8656
                     /gene="cysN"
                     /locus_tag="JQH65_000147"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HAY6137673.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTHIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMSWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKALPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     PSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYPVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPKNEAAVASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            8656..9261
                     /gene="cysC"
                     /locus_tag="JQH65_000148"
     CDS             8656..9261
                     /gene="cysC"
                     /locus_tag="JQH65_000148"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HAY6137674.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            9311..9634
                     /locus_tag="JQH65_000149"
     CDS             9311..9634
                     /locus_tag="JQH65_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HAY6137675.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            9828..10139
                     /gene="ftsB"
                     /locus_tag="JQH65_000150"
     CDS             9828..10139
                     /gene="ftsB"
                     /locus_tag="JQH65_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HAY6137676.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            10158..10868
                     /gene="ispD"
                     /locus_tag="JQH65_000151"
     CDS             10158..10868
                     /gene="ispD"
                     /locus_tag="JQH65_000151"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HAY6137677.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            10868..11347
                     /gene="ispF"
                     /locus_tag="JQH65_000152"
     CDS             10868..11347
                     /gene="ispF"
                     /locus_tag="JQH65_000152"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HAY6137678.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            11344..12393
                     /gene="truD"
                     /locus_tag="JQH65_000153"
     CDS             11344..12393
                     /gene="truD"
                     /locus_tag="JQH65_000153"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HAY6137679.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQHRVNDKELMITAALPGSGEWGTQRKALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            12374..13135
                     /gene="surE"
                     /locus_tag="JQH65_000154"
     CDS             12374..13135
                     /gene="surE"
                     /locus_tag="JQH65_000154"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HAY6137680.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            13129..13755
                     /gene="pcm"
                     /locus_tag="JQH65_000155"
     CDS             13129..13755
                     /gene="pcm"
                     /locus_tag="JQH65_000155"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HAY6137681.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            13895..14986
                     /gene="nlpD"
                     /locus_tag="JQH65_000156"
     CDS             13895..14986
                     /gene="nlpD"
                     /locus_tag="JQH65_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HAY6137682.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGSY
                     SGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITGG
                     NAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPAATTVTA
                     PVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQAI
                     IATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATMG
                     STGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            15049..16041
                     /gene="rpoS"
                     /locus_tag="JQH65_000157"
     CDS             15049..16041
                     /gene="rpoS"
                     /locus_tag="JQH65_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HAY6137683.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(16135..17499)
                     /locus_tag="JQH65_000158"
     CDS             complement(16135..17499)
                     /locus_tag="JQH65_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HAY6137684.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVSFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVATAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMTGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(17588..18364)
                     /locus_tag="JQH65_000159"
     CDS             complement(17588..18364)
                     /locus_tag="JQH65_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HAY6137685.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAMEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFAPHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            complement(18369..19007)
                     /locus_tag="JQH65_000160"
     CDS             complement(18369..19007)
                     /locus_tag="JQH65_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAY6137686.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            complement(19004..20266)
                     /locus_tag="JQH65_000161"
     CDS             complement(19004..20266)
                     /locus_tag="JQH65_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HAY6137687.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALSVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGHPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            complement(20263..21171)
                     /locus_tag="JQH65_000162"
     CDS             complement(20263..21171)
                     /locus_tag="JQH65_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAY6137688.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGDAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            21337..22134
                     /locus_tag="JQH65_000163"
     CDS             21337..22134
                     /locus_tag="JQH65_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY6137689.1"
                     /translation="MSAGQRKEQKLIPVERRQIILEMVAEKGIVSIAELTDRMNVSHM
                     TIRRDLQKLEQQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQP
                     GSCIYLDAGTTTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENR
                     SCVGEAAATMLRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDA
                     TKYGQVATWLALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            complement(22185..22841)
                     /gene="pphB"
                     /locus_tag="JQH65_000164"
     CDS             complement(22185..22841)
                     /gene="pphB"
                     /locus_tag="JQH65_000164"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HAY6137690.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFCPETD
                     LLISVGDNIDRGPESLDVLRLLNQPWFISVKGNHEAMELDAFATGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHHLPHIIEITNDIIKYVIAHADYPGDEYLFGKEIAESELL
                     WPVYRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIR
                     "
     gene            complement(22947..25508)
                     /gene="mutS"
                     /locus_tag="JQH65_000165"
     CDS             complement(22947..25508)
                     /gene="mutS"
                     /locus_tag="JQH65_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAY6137691.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAGLQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     DVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            25584..25718
                     /locus_tag="JQH65_000166"
     CDS             25584..25718
                     /locus_tag="JQH65_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6137692.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            25795..26148
                     /locus_tag="JQH65_000167"
     CDS             25795..26148
                     /locus_tag="JQH65_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HAY6137693.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(26185..28263)
                     /gene="flhA"
                     /locus_tag="JQH65_000168"
     CDS             complement(26185..28263)
                     /gene="flhA"
                     /locus_tag="JQH65_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HAY6137694.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRRILSRPDTLHCSYEEFC
                     ETWPQLVAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(28337..29347)
                     /gene="hypE"
                     /locus_tag="JQH65_000169"
     CDS             complement(28337..29347)
                     /gene="hypE"
                     /locus_tag="JQH65_000169"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HAY6137695.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPCYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(29344..30465)
                     /gene="hypD"
                     /locus_tag="JQH65_000170"
     CDS             complement(29344..30465)
                     /gene="hypD"
                     /locus_tag="JQH65_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HAY6137696.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(30465..30737)
                     /gene="hypC"
                     /locus_tag="JQH65_000171"
     CDS             complement(30465..30737)
                     /gene="hypC"
                     /locus_tag="JQH65_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HAY6137697.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVITEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(30728..31600)
                     /gene="hypB"
                     /locus_tag="JQH65_000172"
     CDS             complement(30728..31600)
                     /gene="hypB"
                     /locus_tag="JQH65_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HAY6137698.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(31604..31954)
                     /gene="hypA"
                     /locus_tag="JQH65_000173"
     CDS             complement(31604..31954)
                     /gene="hypA"
                     /locus_tag="JQH65_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HAY6137699.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEVECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            32166..33797
                     /gene="hycC"
                     /locus_tag="JQH65_000174"
     CDS             32166..33797
                     /gene="hycC"
                     /locus_tag="JQH65_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HAY6137700.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKEGKLWFALGRLGTLLLAI
                     ACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWVPQAHANA
                     SAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIVLLVLGMITAFVGGLYALMEHN
                     IQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSLFKSVLFL
                     GAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEWVIYQSFF
                     KLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAENATCAPLL
                     MSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLIACPLLPF
                     IIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKLRKWLNPV
                     SLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            33800..34723
                     /gene="hycD"
                     /locus_tag="JQH65_000175"
     CDS             33800..34723
                     /gene="hycD"
                     /locus_tag="JQH65_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HAY6137701.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDSGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            34741..36450
                     /locus_tag="JQH65_000176"
     CDS             34741..36450
                     /locus_tag="JQH65_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase large subunit"
                     /protein_id="HAY6137702.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            36460..37002
                     /gene="hycF"
                     /locus_tag="JQH65_000177"
     CDS             36460..37002
                     /gene="hycF"
                     /locus_tag="JQH65_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HAY6137703.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            37002..37769
                     /gene="hycG"
                     /locus_tag="JQH65_000178"
     CDS             37002..37769
                     /gene="hycG"
                     /locus_tag="JQH65_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HAY6137704.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGELDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEVRIR"
     gene            37766..38176
                     /locus_tag="JQH65_000179"
     CDS             37766..38176
                     /locus_tag="JQH65_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HycH family protein"
                     /protein_id="HAY6137705.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            38169..38639
                     /gene="hycI"
                     /locus_tag="JQH65_000180"
     CDS             38169..38639
                     /gene="hycI"
                     /locus_tag="JQH65_000180"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HAY6137706.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(38798..40222)
                     /locus_tag="JQH65_000181"
     CDS             complement(38798..40222)
                     /locus_tag="JQH65_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAY6137707.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LAAKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKALGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(40231..41688)
                     /gene="ascF"
                     /locus_tag="JQH65_000182"
     CDS             complement(40231..41688)
                     /gene="ascF"
                     /locus_tag="JQH65_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HAY6137708.1"
                     /translation="MAKNYAALARSEIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTPRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASADIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYSIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAQARKHQSVQPTVAKEVSLN"
     gene            41948..42958
                     /locus_tag="JQH65_000183"
     CDS             41948..42958
                     /locus_tag="JQH65_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY6137709.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCNAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDYKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIHRDSLIAL
                     SR"
     gene            43107..43634
                     /gene="hydN"
                     /locus_tag="JQH65_000184"
     CDS             43107..43634
                     /gene="hydN"
                     /locus_tag="JQH65_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HAY6137710.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            43787..46039
                     /gene="hypF"
                     /locus_tag="JQH65_000185"
     CDS             43787..46039
                     /gene="hypF"
                     /locus_tag="JQH65_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HAY6137711.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLREDPETFLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPKYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLAAFWQQWLNWQAQVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(46167..47300)
                     /gene="norW"
                     /locus_tag="JQH65_000186"
                     /pseudo
     CDS             complement(46167..47300)
                     /gene="norW"
                     /locus_tag="JQH65_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
     gene            complement(47297..48736)
                     /gene="norV"
                     /locus_tag="JQH65_000187"
     CDS             complement(47297..48736)
                     /gene="norV"
                     /locus_tag="JQH65_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HAY6137712.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNKVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFEELASEAK"
     gene            48923..50437
                     /gene="norR"
                     /locus_tag="JQH65_000188"
     CDS             48923..50437
                     /gene="norR"
                     /locus_tag="JQH65_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HAY6137713.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKGVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
     gene            complement(50434..51399)
                     /gene="gutQ"
                     /locus_tag="JQH65_000189"
     CDS             complement(50434..51399)
                     /gene="gutQ"
                     /locus_tag="JQH65_000189"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HAY6137714.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(51392..52165)
                     /gene="srlR"
                     /locus_tag="JQH65_000190"
                     /gene_synonym="gutR"
     CDS             complement(51392..52165)
                     /gene="srlR"
                     /locus_tag="JQH65_000190"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HAY6137715.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            complement(52232..52590)
                     /gene="gutM"
                     /locus_tag="JQH65_000191"
                     /pseudo
     CDS             complement(52232..52590)
                     /gene="gutM"
                     /locus_tag="JQH65_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
     gene            complement(52695..53474)
                     /gene="srlD"
                     /locus_tag="JQH65_000192"
     CDS             complement(52695..53474)
                     /gene="srlD"
                     /locus_tag="JQH65_000192"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HAY6137716.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLVAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGESMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLVEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            complement(53478..53849)
                     /gene="srlB"
                     /locus_tag="JQH65_000193"
                     /gene_synonym="gutB"
     CDS             complement(53478..53849)
                     /gene="srlB"
                     /locus_tag="JQH65_000193"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAY6137717.1"
                     /translation="MTVIYKTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            complement(53860..54819)
                     /gene="srlE"
                     /locus_tag="JQH65_000194"
                     /gene_synonym="gutE"
     CDS             complement(53860..54819)
                     /gene="srlE"
                     /locus_tag="JQH65_000194"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAY6137718.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            complement(54816..55379)
                     /gene="srlA"
                     /locus_tag="JQH65_000195"
                     /gene_synonym="gutA"
     CDS             complement(54816..55379)
                     /gene="srlA"
                     /locus_tag="JQH65_000195"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAY6137719.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSQGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            55635..56720
                     /gene="mltB"
                     /locus_tag="JQH65_000196"
     CDS             55635..56720
                     /gene="mltB"
                     /locus_tag="JQH65_000196"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HAY6137720.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            56865..57362
                     /gene="pncC"
                     /locus_tag="JQH65_000197"
     CDS             56865..57362
                     /gene="pncC"
                     /locus_tag="JQH65_000197"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HAY6137721.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMVIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            57442..58503
                     /gene="recA"
                     /locus_tag="JQH65_000198"
     CDS             57442..58503
                     /gene="recA"
                     /locus_tag="JQH65_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAY6137722.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            58746..59246
                     /gene="recX"
                     /locus_tag="JQH65_000199"
     CDS             58746..59246
                     /gene="recX"
                     /locus_tag="JQH65_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAY6137723.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYDEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            59374..62004
                     /gene="alaS"
                     /locus_tag="JQH65_000200"
     CDS             59374..62004
                     /gene="alaS"
                     /locus_tag="JQH65_000200"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAY6137724.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKDANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            62239..62424
                     /gene="csrA"
                     /locus_tag="JQH65_000201"
     CDS             62239..62424
                     /gene="csrA"
                     /locus_tag="JQH65_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HAY6137725.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            62740..62832
                     /locus_tag="JQH65_000202"
     tRNA            62740..62832
                     /locus_tag="JQH65_000202"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:62774..62776,aa:Ser,seq:gct)
BASE COUNT        14700 a        16139 c        17169 g        14825 t
ORIGIN      
        1 aggtaggtaa ctcaaaaaac aatactgaac tccccgcaca ggtcgtgaaa cttttgaatg
       61 ccagcaatct aaagcagaca agggtgggct tatggggatt tggttttgat tttgaagata
      121 tgaaggttcg ttgttggtat gaacaccatt tcccgctgtt gttaaacaaa aaagaagacc
      181 taatacctaa gttgcgtctg gcagcgcaag cggcatcaca cattctaagc ttgttacacc
      241 gagcgttgaa agaagcatgg ttctctgaga agaaaaccac aaagcttgat ttcggttttg
      301 tggatattga cttctggaac aaaactcagc atcgattcct gagattagtg cgcaaaattg
      361 aagaaggtca ggatccggac gaattactca gtaaatggca aaaggaaatg tggttattcg
      421 cgcgtcagga ttttgacgat cgtgtcttta ccaatccata tgagcccgtt gatttggaac
      481 gcgtcatgac cgcacgcaag aaatatttta caacatcagc ggagaagcaa aatgctaacg
      541 ccgccaggga gaaaaagcag gaggctgctg aatgagtatt gtaaaagatg agcataaagt
      601 tgcgctgcgt aaatggcatg aagagttgca ggataaacgc ggcgagcgtg ccagtctgcg
      661 gcgtagtacg acggttaatg atgtttgtct gacggatgga tttcgcctgt ttcttaagaa
      721 cagacaaata aagtggcagg atgaacccga gtggcgtatc accgcactag cgctgatagc
      781 agcagttagc gccaatgtta aagctattga cgaacggcag ccttttgccg cgcaactggc
      841 ggcggtaatg aaaaaggggc ggtttactcg tttgttggca gtaaaaacgc cggatgattt
      901 attgcggcag ttacgccgtg cggtcaaatt attaaacggt tccgtcaatc ttgactctct
      961 agaagagggg gtcttccgct ggtgccagga gagcgatgac ctgctcaatc atcaccgccg
     1021 ccagcagcga ccaacagaat ttatccgtat ccgctgggcg ctgggcgctg ggcgctgggc
     1081 gctgggcgct gggcgctgga atattaccag gctggcgacg ccgataacga acaaaattaa
     1141 taaaggacta tctcatgaca acatttattc agcttcattt gttaaccgct taccctgctg
     1201 ccaatcttaa ccgtgatgat tccggctctc cgaaaactgt gattctgggt ggagcaacgc
     1261 gtctgcgcgt ttcctcgcaa agtctgaagc gtgcttggcg cacttctgaa ctttttgaac
     1321 aggcgctggc gggccatatt ggtgttcgta gcgggcgtat tgcgcgtgag gcggcaacta
     1381 tcctgattga gaaaggaatc gaagataaaa aagccatcga atgggcagta gagatagccg
     1441 attatcttgg gaaagacaaa aaacaaaaaa acgacaaaaa accaaaagat ccgctcacta
     1501 gcgccgaaac tgaacagctc gtccatatca gcccggcaga atttgacgcc gtaaaagcgc
     1561 tggcccatca actggcagaa gaaaagcgcg cgccaaaaga gaaagatctc gctttgttac
     1621 gtaaagaccg tatggcggta gatatcgcta tgtttggtcg tatgctggcg aaaaaacccg
     1681 ggtttaatgt agaagccgcc tgccaggtcg cgcatgcgtt tggtgtcagt gaaacgattg
     1741 tcgaaaatga ttttttcacc gccgtagatg atttacgcca ggcttctgaa gatgccggtg
     1801 ccggtcatgt agatgaaacc ggattcggtt ctgcgctgtt ctacacctat atcttatctg
     1861 cattgataaa gatctgctgg tagaaaacct cggcggtgac gaagcgttag ctaatcagac
     1921 catacgcgcc tttacggaag ccgcgcttaa agtctcccca accggtaaac aaaacagctt
     1981 tgccagccgg gcttatgcct cctgggcgct ggcagaaaaa ggcaccgatc aaccgcgttc
     2041 tctggcggca gctttctatg aacccattaa tggcacccgt cagttagagg tggcggtgca
     2101 gcgtattaca actcttcgcg aaaatatgaa tacggtctac gaacagaaga ccgattacgc
     2161 aagttttgac gtgatgaaca aacagggaag catgaaggac gtgctggact ttatctgcgc
     2221 gtaaggggcg gcttatgagc caatatctga tttttcagct tcatgggccg atggcatcct
     2281 ggggcgtcga tgcccccggc gaagtgcgtc atacccatga actgccttcg ctctcagcat
     2341 tgctggggct gctggctgcc ggggtaggga tccggcgtga tgataccgaa cgcttaaacg
     2401 cgtttaatcg ccactattca ctggtggttt gcgccagccg taacccgcgc tgggcacggg
     2461 attatcacac ggtccagatg ccaaaagagg tgcgtaaagc acgttatttc agccgtcgcg
     2521 aagagttgag cgaccctgag cttttgagcg cgattatctc ccggcgcgac tactacaccg
     2581 atgcctggtg gatggtggcg gtggcaacaa cagccgatgc gccttacagc ctcgaacagt
     2641 tgcaggacgg tttacgccat ccagtttttc cgctttatct ggggcgaaaa agtcatccgc
     2701 tggcattacc tcttgcgccg ttactgctcg aaggcaacgc gtgtgatgcc ttatgtaatg
     2761 cataccaaca gtatcaggat catttccata aactgaaagt ttcactcccg aaacttcagg
     2821 atgaatgctg gtgggaaggg aaacacgacg gcctggtcgc gagcaaaata ttacgccgcc
     2881 gggatgttcc tttaaatcgt cagcagtggc tgtttgggga acgcaccgtc aatcaggggc
     2941 cgtggctcag caaggaggaa ccatgtacct ctcaagaata accttacata ccggccagct
     3001 ttcgcctgcg cagttgctgc atctggtgga tcgcggtgaa tatgtgatgc atcagtggct
     3061 gtgggatctc ttccctagcg gcaaagaaag gcaatttctt tatcgtcggg aagaactcca
     3121 gggcgcgttt cgcttttttg tactgtcgca ggaacgcccg gcggaaagtg atacgttcac
     3181 catagaatgc cggtcatttg cgcccgagct gtgtaccgga caacaacttt gtttcaacct
     3241 gcgggcgaat ccaacaatct gtaaagcggg caagcgccac gatctgctga tggaggcgaa
     3301 acggcaggtg agggggcagg cggaaggaag tgatgtctgg ttgcatcaac aacaggcggc
     3361 gctggactgg ctggcagcgc agggggagag aagtggtttt acactgctgg atatctcggt
     3421 tgatgcctac cgacaacagc aactgcggcg ggaaaactcc cggcaactga tccagttcag
     3481 tagcgtggat tatacgggga tgcttacggt caccgatccc gggttatttc tgcaacgtct
     3541 cagtcagggg tatggcaaaa gccgcgcgtt cggctgtggt ctgatgctga tcaaacccgg
     3601 agcagaggcg tgacgtttgt accgctgagc ccgatcccgt taaaagatcg cacctctatg
     3661 atcttcctcc agtacgggca aatcgacgta ctggacggcg ctttcgtgct gatcgataaa
     3721 accgggatcc gcacgcacat tccggtggga tcggtcgcct gcattatgct cgaaccggga
     3781 acgagagttt cccacgcggc ggtgcatctg gccgccacgg tggggacgct acaggtctgg
     3841 gtcggtgaag cgggtgttcg cgtctactct tccggacaac ccggtggggc gcgtgctgat
     3901 aagttactct accaggcaaa gctggcttta acggaagatc tacgtctgaa ggtggtgcgc
     3961 aaaatgtatg aattacgttt tcgtgagcca ccgccagctc gccgttcagt ggagcagcta
     4021 cgtggaattg agggatctcg cgttcgccag acgtatgcat tactggcgaa acaatatggt
     4081 gtgaaatgga atggtcgcaa atacgatcct aaagactggg aaaaaggcga tgttgtgaat
     4141 cgctgcatca gtgctgctac atcatgtctg tacggtatat ctgaagcggc agtattagcc
     4201 gcgggatatg cgcccgctat tgggtttatc catagtggca aaccgctttc atttgtttat
     4261 gacatagccg atatcattaa atttgattcg gttgtaccaa aggcatttga aatagcggcg
     4321 aggcaacccg cagaacctga taaagaagtc agattagcct gtcgtgatat tttccgtagc
     4381 actaagttaa cgggcaaatt aataccgtta attgaggaag tccttgccgc aggtgaaatt
     4441 gaaccgccac aacctgcgcc ggatatgtta ccgccagcca tccccgaacc tgaaacgttg
     4501 ggcgatagcg gtcatcgagg acgcggctga tgagcatggt cgtggttgtt acagaaaatg
     4561 tcccgccacg cttacgtgga cggctcgcaa tctggctact ggaagtgcgt gccggtgtgt
     4621 atgttggtga tacatcaaaa cgtattcggg agatgatctg gcagcaaatt acccaactgg
     4681 ctggttgcgg aaatgtggtg atggcctggg cgaccaatac cgagtcgggt tttgaatttc
     4741 agacctgggg agaaaacaga cgtattccgg tggatttgga tgggttacgt ttggtttctt
     4801 ttcttcctgt tgataatcaa taagttatcc gttctttaaa aataaggaaa tgttttaatt
     4861 tagttggtag attgttgatg cggaataaat ttgtttaaaa acagttatgt atgcttagtg
     4921 tgttccccgc gccagcgggg ataaaccggg cgcactggat gcgataatgg atatcactta
     4981 gaattccccg cccctgcggt agaactccca gctcccattt tcaaacccat caagacgcct
     5041 tcgccagctc cttcaccaac ggtagcatta tccgcataac gtcacggcag cgacgttcta
     5101 ttcttccagg aagtgcctta tcaatatgct gttgattatc cagtcttacg tcatgccagc
     5161 tatttcccgc agggaatgcg gctgtttttg cgcgttgctg ataaccatcc ttattcccaa
     5221 gattccagtt agtcgcttcc accgaaagta cagcaatgcc cgctttgtcg aatatttctg
     5281 cgtcattaca acacccagtg cctttcggat aatttttatt caaccccgga ttggtcgttg
     5341 cggctattcc gtgactgcgc gcaattgcca gcgccctgtc gcgcgttaat ttccttactg
     5401 cttcaggggt ttttacaccg ctgttgaaat acaatttatc gccaacaatt aagttatcga
     5461 gattaatcac cagcagcgta tttttctttt cggtgtcact catccgcttg agtaaattct
     5521 cagcgcctaa tttcccttcc tcttcgccgc tggtcgccac aaatcgaata ccatactcgg
     5581 taggcgtatt tttcaggcgt tctgccaatt ccagcatgac acctaaacct gcggcgttat
     5641 catccattcc ttgtaacgtc agcccgccga gattggcatc ggcgtcagca tcgctcagcg
     5701 gggcgtaagt atccagatgc gccataatga tgatctgctg cggcgctttg ccttcatgag
     5761 cggcaatcac cgtacttccc gtcacgttat gccagctctt acgattatcg cgggcggtat
     5821 aaatataccg actattaaat gtccgaatat cactgcgata acccatttgc tgaaactgtt
     5881 ggcgaatata atcggcagat aacatttctg caggagttcc ggtcatgcgt cccggaaaga
     5941 aagtagcaat atgtcgtgcc tgagtattag caaaatcgcc aggtttaggt gacgaggcgt
     6001 gtacggggag aataaagcat acgccgagcg ccagggcagc ggtacggtgg cgcaatgcgg
     6061 aaaacatagt gagtccttaa ataccatgca aattttttta ccgccatagt atgaaactgc
     6121 cgctgcgcta aaacaatttc aaatcttcct aaacgcccga aatccggtgc cttaagcact
     6181 ttttgatatt agctttgcca aatcgttatt ccgttaagga actactcatt ctaattggta
     6241 atttcattcg ttctcttacg ctccctatag tcgaaacatc tgatggcaag aaaatagcgg
     6301 tattgcaaag gaacggttat ggctcaaaaa cgacttactc acctgcggca actggaggcg
     6361 gaaagcatcc acattattcg cgaggtggcg gcagaattct caaatccggt gatgctctac
     6421 tctatcggta aagattccag cgtcatgctg catctggcgc gcaaggcgtt ttatccaggt
     6481 acgctgcctt tcccgttgct gcatgtcgat accggctgga aattccgcga gatgtatgag
     6541 ttccgcgatc gtactgctaa agcctacggc tgcgagctgc tggtgcataa aaacccggaa
     6601 ggcgtggcga tggggattaa tccattcgta cacggcagcg cgaaacatac cgatattatg
     6661 aaaactgaag gcctgaaaca ggcgctgaac aaatacggtt ttgatgccgc cttcggtggt
     6721 gcgcgccgtg atgaagagaa atcccgcgct aaagagcgaa tctactcttt ccgtgaccgc
     6781 ttccatcgct gggatccgaa aaatcagcgc ccggagctgt ggcacaacta caacgggcaa
     6841 attaacaaag gtgaaagcat ccgcgtcttc ccgctctcta actggaccga gcaggatatc
     6901 tggcaataca tctggctgga aaatatcgac attgtgccac tgtatctcgc tgcggaacgt
     6961 ccggttctgg aacgcgacgg tatgttgatg atgattgatg acaaccgtat cgacctgcaa
     7021 ccgggcgaag tgattaaaaa acggatggtg cgtttccgta cgctgggctg ctggccgctg
     7081 accggtgcgg tggagtcaaa tgcacaaaca ctgccggaga tcatcgaaga gatgctggtt
     7141 tctaccacca gtgaacgtca gggccgcgtg attgaccgcg accaggcggg gtctatggag
     7201 ctgaaaaaac gtcaggggta tttttaagat gaacaccgca cttgcacaac aaatcgccaa
     7261 tgaaggcggc gtcgaagcct ggatgattgc gcaacaacat aaaagcctgc tgcgttttct
     7321 gacctgtggt agcgtcgatg acggcaaaag taccctgatt ggtcgcctgc tgcacgatac
     7381 ccgccaaatc tacgaagatc agctctcatc gctgcataac gacagtaagc gtcacggcac
     7441 ccagggcgaa aagctggatc tggcactgct ggtggacggc ctgcaagctg agcgcgaaca
     7501 gggcatcacc attgacgtgg cttaccgtta tttctctacc gagaagcgta aatttattat
     7561 cgccgacacc ccagggcacg agcagtacac tcgcaatatg gcaactggcg catcgacatg
     7621 tgaactggcg atcttactga tcgatgcccg taaaggcgtg ctcgatcaaa cccgtcgtca
     7681 cagttttatc tccacactgt tggggatcaa acatctggtc gtggcgatca acaaaatgga
     7741 tctggtggat tacagtgaag agacgttcac ccatattcgt gaagattatt tgacctttgc
     7801 cgggcagctg ccgggtaatc tggatatccg ctttgtgccg ctctctgcac tggaaggcga
     7861 caacgtggca tcgcaaagtg aaagtatgtc gtggtacagc ggtccgacac tgctcgaagt
     7921 gctggaaacc gtggagatcc agcgagtggt ggatgcccag ccaatgcgct tcccggtgca
     7981 gtacgttaat cgcccgaatc tcgattttcg tggctacgcc ggaacgctgg catccggtcg
     8041 cgtggaagtc gggcaacgtg tcaaagcgct gccctctggt gtggaatcaa acgtcgcgcg
     8101 gatcgtgact tttgatggtg atcgcgaaga agcctttgcc ggagaagcga tcaccctggt
     8161 gctgacggat gagatcgaca tcagccgtgg cgatctgctg ctggcggcag acgaagcgtt
     8221 accggcggtg ccgagcgcgt cggtggatgt ggtatggatg gcggaacagc cgctttcccc
     8281 gggccagagt tacgacatca aaattgccgg taagaagacg cgcgcgcgtg ttgatggcat
     8341 tcgctatccg gttgatatta ataaccttac ccagcgtgaa gttgaaaacc tgccactgaa
     8401 tgggatcggc ctcgtggatc tcacttttga cgagccgctg gtgttagatc gttatcaaca
     8461 aaatccggtg acgggtgggc tgatttttat cgatcgcctg agcaacgtga ctgttggtgc
     8521 cggtatggtg catgagccga aaaacgaggc cgccgttgcg tcatctgaat tcagtgcatt
     8581 cgaactggaa ttgaatgccc tggttcgtcg ccactttccg cactggggcg cgcgcgattt
     8641 gctgggggat aaataatggc gctgcatgac gaaaacgtcg tctggcatag ccatccggtc
     8701 actgtgcaac aacgcgagct acaccacggt catcgtggtg tagtgctgtg gtttaccggc
     8761 ctctctgggt ccggtaaatc aacggtcgcc ggggcgctgg aggaggcgtt acataaactc
     8821 ggcgtcagta cgtatctgct ggatggcgac aatgttcgcc acggattatg cagcgatctc
     8881 ggttttagcg atgccgatcg taaagagaat atccgtcgcg tcggtgaagt ggcgaatttg
     8941 atggttgaag ccggactggt ggtgctgacc gcatttatct cgccacaccg cgccgaacgc
     9001 cagatggttc gcgaacgcgt aggagaaggg cgctttatcg aagtgtttgt cgatacgccg
     9061 ctggcgattt gcgaagcccg cgatcccaaa ggcttatata agaaagcgcg tgccggtgaa
     9121 ctgcgcaact ttacgggaat agattccgtt tacgaagcgc ctgaatcggc agaaattcat
     9181 ctcaatggtg aacaattagt aacaaatttg gtacagcaat tattagatct gttgagacag
     9241 aacgatatta tcagatcctg agacaccacc gggtttccat gcccggttcg tcaaagtaac
     9301 aggatcaaat atgcgtaata gccataacat tacactaaca aataacgaca gccttactga
     9361 ggatgaagaa accacatggt cactacctgg tgccgtggtc ggttttatct cctggttatt
     9421 tgcgctggcg atgccgatgt tgatttatgg ctctaacacg ctgttcttct ttatctacac
     9481 ctggcctttc tttctggcac tgatgcctgt cgcggtagtg gtggggattg cgctgcattc
     9541 attgatggac ggaaagctac gctacagtat tgttttcacc ctggtgacag taggcattat
     9601 gtttggcgca ctgtttatgt ggctactggg ctaatttgta ctttcttgtc tcctctgttc
     9661 ataattcaaa ccgtaactaa taatgagatt atgttctgca cgccctaggt atacgtaaca
     9721 atggacaaat gtggtacatt tgcccgcgtt gtcgcggtat ccccaacaga ggatgtagag
     9781 tcgtcttcgg atgcatggga tgatgatgcc gtttttcagg gggcaggatg ggtaaactaa
     9841 cgctgctgtt gctggctatt ctggtctggc tacagtattc gctgtggttc ggtaagaacg
     9901 gtatacatga ctatacccgc gtcaatgatg atgtggcggc acagcaagct acaaacgcga
     9961 aacttaaagc gcgaaacgat caactttttg ccgaaattga cgatctcaat ggcggccagg
    10021 aggcgctcga agagcgtgcg cgtaatgaac tcagcatgac caggccgggc gaaacttttt
    10081 atcgtctggt gcctgacgcg tcgaagcgcg cacagtctgc ggggcaaaac aatcgataaa
    10141 tcagcccggg aattaacatg gcaaccactc atttggatgt ttgcgccgtg gttccggcgg
    10201 ccggatttgg ccgtcgaatg caaacggaat gtcctaagca atatctctca atcggtaatc
    10261 aaaccattct tgaacactcg gtgcatgcgc tgctggcgca tccccgggtg aaacgtgtcg
    10321 tcattgccat aagtcctggc gatagccgtt ttgcacaact tcctctggcg aatcatccgc
    10381 aaatcaccgt tgtagatggc ggtgatgagc gtgccgattc cgtgctggca ggtttgaaag
    10441 ccgctggcga cgcgcagtgg gtattggtgc atgacgccgc tcgtccttgt ctgcatcagg
    10501 atgacctcgc gcgattgttg gcgttgagcg aaaccagccg cacgggaggg atcctagccg
    10561 caccagtgcg cgatacgatg aaacgtgccg aaccgggcaa aaatgccatt gctcataccg
    10621 ttgatcgcaa cggcttatgg cacgcgctga cgccgcaatt tttccctcgt gagctgttac
    10681 atgactgtct gacgcgcgct ctaaatgaag gcgcgactat taccgacgaa gcctcggcgc
    10741 tggaatattg cggattccat cctcagttgg tcgaaggccg tgcggataac attaaagtca
    10801 cgcgcccgga agatttggca ctggccgagt tttacctcac ccgaaccatc catcaggaga
    10861 atacataatg cgaattggac acggttttga cgtacatgcc tttggcggtg aaggcccaat
    10921 tatcattggt ggcgtacgca ttccttacga aaaaggattg ctggcgcatt ctgatggcga
    10981 cgtggcgctc catgcgttga ccgatgcatt gcttggcgcg gcggcgctgg gggatatcgg
    11041 caagctgttc ccggataccg atccggcatt taaaggtgcc gacagccgcg agctgctacg
    11101 cgaagcctgg cgtcgtattc aggcgaaggg ttataccctg ggcaacgtcg atgtcactat
    11161 catcgctcag gcaccgaaga tgttgccgca cattccacaa atgcgcgtgt ttattgccga
    11221 agatctcggc tgccatatgg atgatgttaa cgtgaaagcc actactacgg aaaaacttgg
    11281 atttaccgga cgtggggaag ggattgcctg tgaagcggtg gcgctactca ttaaggcaac
    11341 aaaatgattg agtttgataa tctcacttac ctccacggta aaccgcaagg caccgggctg
    11401 ctgaaagcca atccggaaga ctttgtggtg gtggaagatt tgggctttga gcctgatggt
    11461 gaaggtgagc atattctggt tagaatcctc aaaaacggct gcaatacccg ttttgtggcg
    11521 gatgcactgg cgaaattcct gaaaattcat gcccgtgaag tcagcttcgc tgggcaaaaa
    11581 gacaaacatg ctgttacgga acagtggtta tgcgctcgcg tgccaggcaa ggaaatgccc
    11641 gatctgagcg cctttcaact ggaaggctgc caggtgctgg agtatgcgcg gcacaagcgc
    11701 aagctgcgtt taggcgcgct gaaaggtaac gcctttaccc tggttctgcg cgaagtgagc
    11761 aatcgcgatg acgttgaaca acgtctgatc gatatttgcg taaaaggtgt accgaactac
    11821 ttcggtgccc aacgttttgg gattggcggt agcaacttgc agggcgcgct gcgctgggcg
    11881 caaaccaata ctccggtgcg cgatcgcaat aaacggagtt tttggttgtc ggcagcccgc
    11941 agtgcgttgt ttaatcagat tgttgctgag cgcctcaaaa aagcagacgt taatcaagtt
    12001 gttgacggcg atgcgctaca attagccgga cgtggtagct ggtttgtagc aaccaccgaa
    12061 gaactggcgg aattacagca tcgcgtcaac gataaagagc tgatgataac cgccgcattg
    12121 ccgggcagtg gcgaatgggg aactcagcgt aaagcgctgg cattcgaaca agcagctgtc
    12181 gccgcagaaa ctgaattaca agctttactg gtgcgcgaaa aagttgaagc cgcgcgcaga
    12241 gcgatgctgc tgtatccgca acaattaagc tggaattggt gggatgacgt caccgtagag
    12301 atccgtttct ggcttccggc gggtagtttt gcaaccagcg ttgtcaggga acttatcaac
    12361 acaacaggtg attatgcgca tattgctgag taatgatgac ggggtacatg cacccggtat
    12421 acaaacgctg gcgaaagcct tgcgtgagtt tgctgacgtt caggtggtcg cccccgatcg
    12481 taaccgcagc ggcgcttcaa attctctgac actggaatcc tccctgcgca cgtttacctt
    12541 tgaaaatggt gatattgctg tgcaaatggg aaccccgacc gattgcgtct atcttggcgt
    12601 gaatgctctg atgcgtccgc gcccggacat tgttgtgtcc ggcattaacg ccgggccgaa
    12661 tctgggggat gacgttattt attccggtac ggtagccgcc gcgatggaag gccgtcattt
    12721 aggttttccg gcgctggccg tctcgcttga cgggcataaa cattacgaca ctgccgcggc
    12781 ggtaacctgt tcaattttgc gcgcactgtg taaagagccg ctgcgcaccg gacgtattct
    12841 taatattaac gttccggatt tacccttgga tcaaatcaaa ggtattcgcg tgacgcgctg
    12901 cggtacacga catccggcag atcaggtgat cccgcagcaa gatccgcgcg gcaatacgct
    12961 gtactggatt ggcccgccgg gcggtaaatg tgatgccggt ccggggaccg attttgctgc
    13021 ggtagatgag ggctatgtct ccatcacgcc gctgcatgtg gatttaactg cgcatagcgc
    13081 gcaagatgtg gtttcagact ggttaaacag cgtgggagtt ggcacgcaat ggtaagcaga
    13141 cgcgtacaag cacttctgga tcaattacgt gcgcaaggta ttcaggatga gcaggtgctg
    13201 aatgcacttg ctgccgtgcc gcgtgaaaaa ttcgttgatg aagcgtttga acaaaaagcc
    13261 tgggacaata tcgccttgcc gataggtcag gggcagacaa tttcgcagcc gtatatggtg
    13321 gcgcgaatga ccgaattact cgagctgacg ccgcagtcgc gggtgctgga aattggcacc
    13381 ggttcgggat atcaaacggc aatcctggcg catcttgtcc agcatgtttg ctcggtcgaa
    13441 cggattaaag gtttgcagtg gcaggcgcgt cgccgcctga aaaatcttga tttacataat
    13501 gtttcaaccc gtcatggcga tggatggcaa ggttggcagg cacgcgcgcc gtttgacgct
    13561 atcattgtta cggcggcacc gccggaaatt ccaactgcgc taatgacgca gctggacgaa
    13621 ggcgggattc tcgtcttacc cgtaggggag gagcaccagt atttgaaacg ggtgcgtcgt
    13681 cggggaggcg aatttattat cgataccgtg gaggccgtgc gctttgtccc tttagtgaag
    13741 ggggagctgg cttaaaacgt gaggaaatac ctggattttt cctggttatt ttgccgcagg
    13801 tcagcgtatc gtgaacatct tttccagtgt tcagtagggt gccttgcacg gtaattatgt
    13861 cactggttat taaccaattt ttcctggggg ataaatgagc gcgggaagcc caaaattcac
    13921 cgttcgccgc attgcggctt tgtcactggt ttcgctatgg ctggcaggct gttctgacac
    13981 ttcaaatcca ccggcaccgg tcagctccgt taatggcaat gcgcctgcaa ataccaattc
    14041 tggtatgttg attacgccgc cgccgaaaat ggggacgacg tctacagccc agcaaccgca
    14101 aatccagcca gtgcagccag tagctcagca gccggtacag atggaaaacg gacgcatcgt
    14161 ctataaccgt cagtatggga acattccgaa aggcagttat agcggcagta cctataccgt
    14221 gaaaaaaggc gacacacttt tctatatcgc ctggattact ggcaacgatt tccgtgacct
    14281 tgctcagcgc aacaatattc aggcaccata cgcgctgaac gttggtcaga ccttgcaggt
    14341 gggtaatgct tccggtacgc caatcactgg cggaaatgcc attacccagg ccgacgcagc
    14401 agagcaagga gttgtgatca agcctgcaca aaattccacc gttgctgttg cgtcgcaacc
    14461 gacaattacg tattctgaat cttcgggtga acagagtgct aacaaaatgt tgccgaacaa
    14521 caagccagct gcgaccacgg tcacagcgcc tgtaacggta ccaacagcaa gcacaaccga
    14581 gccaactgtc agcagtacat caaccagtac gcctatctcc acctggcgct ggccgactga
    14641 gggcaaagtg atcgaaacct ttggcgcttc tgaggggggc aacaagggga ttgatatcgc
    14701 aggcagcaaa ggacaggcaa ttatcgcgac cgcagatggc cgcgttgttt atgccggtaa
    14761 cgcgctgcgc ggctacggta atctgattat catcaaacat aatgatgatt acctgagtgc
    14821 ctacgcccat aacgacacaa tgctggtccg ggaacaacaa gaagtgaagg cggggcaaaa
    14881 aatagcaacc atgggtagca ccggaaccag ttcaacacgc ttgcattttg aaattcgtta
    14941 caaggggaaa tccgtaaacc cgctgcgtta tttgccgcag cgataaatcg gcggaaccag
    15001 gcttttgctt gaatgttccg tcaagggatc acgggtagga gccaccttat gagtcagaat
    15061 acgctgaaag ttcatgattt aaatgaagat gcggaatttg atgagaacgg agttgaggtt
    15121 tttgacgaaa aggccttagt agaagaggaa cccagtgata acgatttggc cgaagaggaa
    15181 ctgttatcgc agggagccac acagcgagtg ttggacgcga ctcagcttta ccttggtgag
    15241 attggttatt caccactgtt aacggccgaa gaagaagttt attttgcgcg tcgcgcactg
    15301 cgtggagatg tcgcctctcg ccgccggatg atcgagagta acttgcgtct ggtggtaaaa
    15361 attgcccgcc gttatggcaa tcgtggtctg gcgttgctgg accttatcga agagggcaac
    15421 ctggggctga tccgcgcggt agagaagttt gacccggaac gtggtttccg cttctcaaca
    15481 tacgcaacct ggtggattcg ccagacgatt gaacgggcga ttatgaacca aacccgtact
    15541 attcgtttgc cgattcacat cgtaaaggag ctgaacgttt acctgcgaac cgcacgtgag
    15601 ttgtcccata agctggacca tgaaccaagt gcggaagaga tcgcagagca actggataag
    15661 ccagttgatg acgtcagccg tatgcttcgt cttaacgagc gcattacctc ggtagacacc
    15721 ccgctgggtg gtgattccga aaaagcgttg ctggacatcc tggccgatga aaaagagaac
    15781 ggtccggaag ataccacgca agatgacgat atgaagcaga gcatcgtcaa atggctgttc
    15841 gagctgaacg ccaaacagcg tgaagtgctg gcacgtcgat tcggtttgct ggggtacgaa
    15901 gcggcaacac tggaagatgt aggtcgtgaa attggcctca cccgtgaacg tgttcgccag
    15961 attcaggttg aaggcctgcg ccgtttgcgt gaaatcctgc aaacgcaggg gctgaatatc
    16021 gaagcgctgt tccgcgagta agtaagcatc tgtcagaaag gccagtctca agcgaggctg
    16081 gccttttctg tgcacaataa aaggtccgat gcccatcgga cctttttatt aaggtcaaat
    16141 taccgcccat acgcaccagg taattaagaa tccggtaaaa ccgagaatgg tcgttaacac
    16201 tgtccaggtt ttcagaccgt ctgctaccga caaccccaga tatttggtca caatccagaa
    16261 ccctgagtca ttaatatgtg acgcgccaag cccaccaaag caggctgcca gcgtcaccaa
    16321 tacgcactga atcggattca atcccatcac cgcttctgag agtaacccgc cggttgtcag
    16381 gattgctacg gttgctgacc cctgcgatgc acgcagagcc agtgaaataa taaatgcggc
    16441 tggtaacaga ggcaggtcaa tcatttgtag catattggca agggctttgc cgacgcccga
    16501 ttccaccagc actttgccaa atacccctcc agcaccagta accaaaatca ctaccgccgc
    16561 agtgggaagg gctgagccca taatgtcgct ggtgtgttgt aagctccagc cgcgacgtaa
    16621 agccaataac cagaatgcca gcaccagcgc aatcattaga gctaccattg gtgagccgat
    16681 cagctgtagc gtaccaagca ggggatgcga aggcggcatc agtgttgcgg aaaccgtacc
    16741 cgtcatgata atcgcgatag gaataacaat tagcgaggtg accagcgcga cgcccggtgg
    16801 atttatttta tcgcttaatt ttgtcgcgcc ttcctcactg gccggagcca gttgcatctg
    16861 ttccagtact tctactgaca tcgcatattg gcgcttattg attattttcg ctgcaaagta
    16921 gccaacaatc cctacgggaa tagaaatcgc aataccgatg atggttagcc agccgatgtc
    16981 tgcgtggagt aaccccgctg tggcgacagg gcctggatgc ggcggtactg ccacatgaac
    17041 agttagcatg attccagcga caggcaggcc aaatttgagt ggcgatattt tggcaacctt
    17101 ggcaaaaccg taaatgattg gcgcaagaat aataaagctg acatcaaaga agacgggaat
    17161 accgaggaag aacgctgcca gagtcagcgc agcgatagtt cgtttgtcac ctaacttgcg
    17221 actgaaataa ttagccagtg actctgcacc accagagtgt tcgatcatac gccccagcat
    17281 agcgcccaga ccaataataa tagtgacgga accaagcaca ccgcccatcc cggcgatcat
    17341 cactttaccc acttcgcccg ccggtatacc cgccgcaagt gcgactaaca ggctgacgag
    17401 gagcagagca acgaatggtt gtacctttgc cttgatgacc agcagcaaca gcatgattac
    17461 gccagctaac gcaatgcata acaatgtaat tgtggacatg ggaaaccctg tctgaaagtt
    17521 atagttaacc taccccatcc gtagatgggg ggatgtatgg gtacgttgta attagggatt
    17581 taacgaatta gcgccaggcg tcaaaccagc caagcccttc ttcggtgagg ccacgaggtt
    17641 tatattcaca accgatccag ccctgatatc ccacctcatc gaacaggcgg aacagccacg
    17701 gatagttgat ttctccatcg tccggttcat gtcgatcagg tagtccggca atttgtacgt
    17761 gcgcatattt cccagcgtag tcgcggatta aatgcgtcag gttgccatct actttttgcg
    17821 catgaaaagt atctagttga ataaacacgt tatctcgcgc aacctcttca acaatagcca
    17881 gtgcctgata ctggctggag aagagataat gaggcttaac gtcggggctg agtgcttcaa
    17941 ctaatattcg cttgccgtgt ggcgcaaagc ggtcggcagc gtagcggaga ttatcgataa
    18001 atactgcccg gtaccgttca gcatcttcgc cagcgggcac gacgcctgcc atcacatgga
    18061 cttgttcaca attgagcgcc aatgcatatt ccagtgccag gtcgatgtct gcgtgtgctt
    18121 cgtgctcacg tccgggaagg gcggataatc cccattcccc cgcattaata tctccgggag
    18181 cggtattgaa cagcgccagt gtcagatggt tttgctccag ttgcttttgg atttgcaggg
    18241 tggagtagtc atagggaaac agaaattcca tagcatcgaa cccggctttt cgcgctgcgg
    18301 cgaagcgttc aataaaaggc acttcggtga acatcatgga taaattagct gcaaaacgag
    18361 gcattgcatt aactccttaa ttccgcaatt tcacctgcgg tcagataacg gatcgggcgg
    18421 tcaccgagaa taaaaatcag ctttgccgtt tcctccagct cttccatatt gttggcggct
    18481 tcttgcaggc tttcaccgca aaccactggg ccatgatttg ccagtaaaaa agcctgattg
    18541 tctgctgcca gttccgccag atcctgtgcg atgcgtttat cgcccggtcg gtaatagggc
    18601 accagcggga catttcccat ccgcatcacc acgtatggtg tgaacggacg aataacgttg
    18661 ctgctgtcca gcccttgcag gcaggaaagc gccgtcgacc atgtgctgtg caaatgcacc
    18721 accgctttac agcgcggatt gttgcgatac agcgccagat gaaagagcac ctctttcgag
    18781 ggtttgtcac cacttaacca ttcgccatcc gcggcgactt tggaaagccg ctgcggatcg
    18841 agattgccca ggcatgaacc tgtcggtgtc gccagtaaat tcccgtcagg taaaagcagc
    18901 gacagattgc cagccgaacc ggttgcatag ccgcgctgaa agaatgaact ggcaatccgc
    18961 gtcatctcct ctcgcaaaga ctgctctact tttgcgaaat cgctcatgat aaaaactctc
    19021 tttgggctcg tgaaaaaaag gcttcatcac cgaagttgcc agatttaagg gcgagtgaga
    19081 caggcttatc cagtgcgttt acccacggca cgccggggga aatggttggg ccaatatgaa
    19141 acccttttat gcccaggctc tgtgtgacta cgccggaggt ctcaccgcct gcgacaataa
    19201 agcgtgtcac gccttccgct gctaaccgcg ccgctagttg agaaaacaga gtttctactg
    19261 cctgactggc tttttgtgca ccgtattgct gttgaattgc tgccaatgcg tcagtgctgg
    19321 cggtggcaaa aaccagtgga gcaagtacac tttcctggcc cagaacccac tctgccagtt
    19381 cgtgtgcata agcggccaga gtttcggttg agaggcagcg tgccacatca acttcacggg
    19441 ctggtgcaat ttgacggtaa tgtgccacct ggcggttggt catttgagag catgaaccgg
    19501 agagcactac gccgcgccca gcgagcggat gccctgcttc gcgagcctgg ttaccgtttt
    19561 cttgcgccca ctgccgggcc aggccaatcg ccagaccaga accgcccgtt accagtgggg
    19621 catcgcgcaa ggcttctccc tgaatttcca gatggtgttc ggtcagcgca tcaagcaccg
    19681 cgtagcggta gccctcttgc tgtaagcgag ccagctcttg acgaacggca tccacacctt
    19741 gttcgaaaac atgtgccgaa acgacgccgc agcgccctgt ggattgcgct tcaaccagac
    19801 ggggaagata gctgtcggtc atgggattta ccgggtgatg gcgcatcccg gattcggcca
    19861 gcagttgatt cattacgaac aaatacccct gataaaccgt acgtccgttg accgacaggg
    19921 ccggagagaa gaccgtaaac ggcgtgtcga gagcatccat taatgcatcg gtaaccgggc
    19981 cgatattacc tttcgccgta ctgtcgaaag tagagcagta tttgaaatag atctgtttgc
    20041 aaccttgctg ttgcaaccag ctcagagccg ccagcgattg ctgtgtggct tcaaccaccg
    20101 gacaggagcg cgttttcagg ctgatcacca gtgcgtcgat tgcttccggc attttacctg
    20161 ttggaacacc gttaatttgt accgttggta gaccgttttc caccagaaaa ctggcgatat
    20221 ccgtcgcgcc ggtaaaatca tcggcgataa cgccaatctt gatcatgatt tcgctcccgg
    20281 tagagtgatg ccagagaaaa tcttgataac tgcgctatcg tcttctttcc cgtaacctgc
    20341 gttactggcg ctggtgaaca tattcaatgc tgttgaggcc aatggcagcg ggaagtgcag
    20401 ggctttggct gtatcggcaa ccagaccaag atccttaaca aaaatatcga cggctgaatg
    20461 cggggtgtaa tcgccatcca ccacatgacg catccggttt tcgaacatcc aggaatttcc
    20521 ggcggcattg gtcacgacgt catacatcac atccagcggg atccccgcac gggctgcaag
    20581 tgccatcgct tcggctccgg cagcaatatg tacgcccgct aacaactggt gaataatttt
    20641 tacggtcgaa cctagtcccg gttctgcacc tatgcgataa acttttccgg caacggcttc
    20701 cagcacgggt gccagtcgtt caaaggcaat atcgctaccg gaggccatga cagtcatttc
    20761 accgttagcg gcttttactg catcaccaga aactggcgca tccagcattt ccagatcgaa
    20821 tccagccaga gcggtagcaa tttcttgcgc atcagcacta gcgatagtgg aagaaaccat
    20881 tactgccgta ccgggtttca gatgttgtgc aacgcctgtt tcaccaaaca gcacctgttt
    20941 aacctgggcc gcattgacca ccagcaccag cagagcgtcc agtttttcgg caaacgtcgc
    21001 ggcgttatca gaaaccccgc aagcacctgc ctctttcaac gtagcgcagg cattgctgtt
    21061 caggtctgcg ccccaggtag aaagacctgc gcggacacat gacagtgctg ctcccattcc
    21121 cattgaccct aagccaacga taccgacatg aaactcagat cccgttttca tctgctctcc
    21181 ttgttaattt aagtgatatt ttgtttgata ttgtgaatat aagcgctaga agataacgat
    21241 atggtgagcc gattcacata aattaacatt gtgtgttatt ttatgtgaac taagcgttag
    21301 ttgcgccgcg cacgtttcgc aggcaaatag cgtagaatgt cagcaggaca aaggaaggag
    21361 caaaagttga tacccgtaga gcgtcgccaa atcatccttg agatggtagc tgaaaaaggc
    21421 attgtcagta ttgctgaact aacggacaga atgaatgtat cacatatgac cattcgtcgg
    21481 gatttacaaa aactggagca gcagggagcc gttgtgctgg tgtccggagg cgtccagtct
    21541 ccgggacgcg tggcgcatga accttctcat caggtaaaaa ctgcgctagc aatgacgcaa
    21601 aaagcggcta ttggcaagct ggcggcaagt cttgttcagc cgggaagttg tatctatctg
    21661 gatgcaggaa cgaccacgtt agcgatagca cagcatctga ttcacatgga gtcactgact
    21721 gtggtcacaa acgatttcgt tattgcgaac tacttgctcg acaacagtaa ttgcacaatt
    21781 attcacactg gcggtgcagt gtgtcgggaa aaccgttcct gtgtcgggga agccgctgcg
    21841 accatgctgc gcagcctgat gattgatcag gcttttattt ctgcatcgtc atggagtgtg
    21901 cgggggattt ctacgccagc ggaagataaa gtcacggtga aacgggcgat tgctagtgcc
    21961 agccgccagc gagttttggt ctgtgatgcg acgaaatatg gtcaggtggc gacatggctg
    22021 gcgttaccat taagcgagtt tgatcagatt attacagacg acggtttgcc ggagagtgcc
    22081 agtcgcgcgc tggcgaagca ggatctctct ttgctggtag cgaaaaatga ataatggcct
    22141 gcaataacat ctggttactc atgcttcaca gaagaagcat gagactacct tattttataa
    22201 aatgacagcc gcccactttt cggcgatccg gtatcaatat aaatctggtt agcgaacgtc
    22261 tgaatgttat caaacatcat atgtccaaat ataaaataat cagcgccgtt tatttgttgt
    22321 aactcgccat taagcgattt ctgcacacga taaacaggcc agagtaattc gctctccgct
    22381 atttctttgc caaagagata ttcatcaccc ggataatctg catgtgcgat gacatatttt
    22441 attatgtcgt tagtgatttc aataatatgt ggaaggtgat ggaatttcag caacagatct
    22501 attgcctctt gttgctctga atcatttaaa tcgaaaaacc agtcaccacc gctggcaagc
    22561 cacatattgc catcgccggt cgcgaacgca tccagctcca ttgcttcgtg gttgccttta
    22621 acggagataa accagggctg gtttagcagg cgcaggacgt caagactctc cggcccacga
    22681 tcaatattat cgccgacaga aataagtaag tcggtttcag ggcaaaaaga gagttgatgt
    22741 aagcgggatt gtaataattg atattcacca tgtatatcac caacgaccca tatatggcga
    22801 tagtgatggg cattaatttt ttgatagcgt gtagatggca tggttttacc ctgtaaaata
    22861 agctttccta ttatacaggg ggtttttatt ttattcgtca gttgtcgtta atattcccga
    22921 tagcaaaaga ctatcgggaa ttgttattac accaggctct tcaagcgata aatccattcc
    22981 agcgcctgac gcggagtcag tgaatccggg tccaggtttt ccagagcttc gaccgctggc
    23041 gaggtttctt ccggtactga cagcaaagac atttgcgtac catccacttg cgtagcggcg
    23101 gcgttcggcg aaatgctttc cagctcacgc agtttttgcc gtgcgcgctt aatcacctct
    23161 tttggcacgc ctgccagagc tgcaaccgcc aggccgtagc ttttgctcgc cgcgccatcc
    23221 tgcacgctat gcataaaggc aatggtgtct ccgtgctcca gcgcatcgag atgcacgttg
    23281 gcgacgtctt ccattttctc cggtagctgg gtcagctcga aatagtgggt ggcaaacagc
    23341 gtcaatgcct taatcttatt cgccagattt tctgcgcacg cccacgccag cgacagacca
    23401 tcgtaggtgg acgttccacg cccgatctca tccattaaca ccagactgta ttcggtggcg
    23461 ttatgtaaaa tattggcggt ttcagtcatc tccaccataa aggttgagcg cccggaagcc
    23521 aggtcatctg ccgcgcctac gcgggtaaag atgcgatcga taggtccaat ctcgactttt
    23581 tgtgccggta catagctgcc aatgtaggcc atcagcgcaa tcagtgcggt ctggcgcata
    23641 taggtacttt taccgcccat gttcgggccg gtaataatca acatacggcg ctgcggcgac
    23701 agattcagcg ggttagcgat aaatggctca ttcagtactt gttcaactac cggatggcga
    23761 ccttcggtaa tgcgaatgcc cggtttatca atgaaggtcg ggcaggtgta gttcagggta
    23821 taggcccgtt ccgccaggtt aaccagcacg tcgagttccg ccagcgcgct cgcgctctgt
    23881 tgcaacgctt ccagatgcgg caacagcagg tcgaacagct cttcataaag ctgtttttcc
    23941 agtgccagtg ctttgccttt tgaggtgaga actttatctt cgtactcttt tagctctgga
    24001 atgatgtagc gctcggcgtt tttcagcgtc tggcgacgca tgtagttgat gggtgccaga
    24061 tggctttgcc cacggctgat ttgaatgtag tagccgtgca ccgcattaaa gccaactttc
    24121 agcgtgtcca ggccggtacg ttcacgctcg cggacttcca gacgctccag ataatcggtc
    24181 gcgccgtcag ccagcgcgcg ccactcatcc agctcttcgt tatagcccga tgcgataaca
    24241 ccaccgtcgc gtaccagcac cggcggtgtg tcgatgattg ctcgctccag cagatcgcgc
    24301 agctcggcaa actcgcccat cttctcacgt agcgcctgta ccggtgcact atcgacagtt
    24361 tctaactgtg cacgcagctc cggcagttgc tggaaagcgt ggcgcatacg ggccagatcg
    24421 cgtgggcgag cagttcgtaa agccagacgt gccagaatac gttcaaggtc gccgacctga
    24481 cgcagtaccg gctgtagccc ggcggtgaaa tcctgcaatg cgccaatagt ttgctggcgc
    24541 tcaagcaaca cgcgggtatc gcgcactggc atatgcagcc agcgtttcag catacgacta
    24601 cccatcggcg tgacggtgca gtcgagcaca gaagccagcg tattttccgc accgcccgcc
    24661 aggttctgag taatttccag gttacgacgt gtcgcggcat ccataatgat gctgtcctgc
    24721 tcacgttcca tggtgataga acgaatatgc ggcagggtcg tgcgttgggt atctttcgca
    24781 tactgcaaca gacaaccggc agcacaaagt ccgcgcggcg cgttctcgac gccaaaaccg
    24841 accagatcgc gggtgccaaa ttgcagattc aactgctggc gcgcggtgtc gatttcaaac
    24901 tcccacagcg ggcgacggcg caggccgcga cggccttcaa tcagcgacat ctcggcgaaa
    24961 tcttctgcat acagcagttc cgccggatta gtgcgttgca gctctgccgc catcgtttcg
    25021 cggtcggccg gttcgctcag gcgaaaacgc ccggagctga tatccagcgt cgcgtagccg
    25081 aaacctttgc tgtcctgcca gatagccgcc agcaggttgt cctgacgctc ctgtaacagg
    25141 gcttcatcgc tgatggtgcc tggcgtaacg atacgcacaa ctttgcgctc aaccggacct
    25201 ttgctggtcg ccggatcgcc aatttgttcg cagatggcaa cggactctcc ctgattcacc
    25261 agtttggcga gatagttttc caccgcatgg tagggaatcc ccgccatcgg gatcggctct
    25321 cccgccgaag caccgcgttt ggtcagtgaa atatccagca gttgcgacgc gcgttttgcg
    25381 tcgtcataaa acagttcata aaaatcaccc atccggtaaa acagcaggat ctcgggatgc
    25441 tgggctttca gcctgagata ctgctgcatc atgggcgtat gggcgtcgaa attttctatt
    25501 gcactcatgg ggttatgtcc ggttccctga tattaaatgg ggtgtgatgg tttttatttt
    25561 cgttgtaatc acataaggcg cacatgatac cggagttaat catttcaggg gaagaagcga
    25621 acggcgagtt cttgatgctg gttccgggaa agatgctgtt ttgcagcttt tgcgtgatgc
    25681 ttatgaagca agtacgcaaa attgcgtcgg ttgaataaac ctcctccttc agagccatag
    25741 tcccctttca aattaagttg taatataaat acaccttaag agtatggagg cagcatgtcc
    25801 ggcaagcgta tctctcgtga aaaactgacg attaaaaaaa tgatcgatct ttatcaagcg
    25861 aaatgcccgc aggcgtcagc ggagccggag cattacgagg cgttgtttgt ttacgcgcaa
    25921 aagcggctgg ataaatgtgt gttcggcgaa gagaaaccag cctgtaagca gtgtccggtg
    25981 cactgttacc agcctgcaaa gcgtgaagag atgaaacaga ttatgcgttg ggccgggccg
    26041 agaatgctgt ggcgtcatcc gatcttaacc gtgcgtcatc tcatcgatga caagcgtccg
    26101 gtgccagaac tgccggaaaa atatcgtccg aaaaaacctc gtgaatagta tgccggacag
    26161 gcagatctgt ccggcaattt gcagttaaat caatgccgat ttatcaattc ccagccgctt
    26221 catccgtgac agcagggtcg tgcgtttcag ccccagacgt tgcgcagcgc ctttaggccc
    26281 ggcaaccacg ccgttggttt ctttcagcac gcgaacaatc aactgatatt catcttcgcc
    26341 ctcctgagcg acaaccgttg cggcaggcgg cgtttcaggc tccggtaaag caatatctgg
    26401 caatgacagc tgcagcacgt tgccgcgtgt tagcaatact gcgcgctcaa tgacgttttc
    26461 cagttcgcgt acgttacccg gccactccat gttgctcaag gtgcgcagcg tctcggctgg
    26521 aatgctgtcg atattgcgcc ccagacgacg ggcaattttg aaggtaaagg ctttcgccag
    26581 cagcggaata tcttccggac gctcgcgtag tggcggcagg tgaatcggga atacgttcag
    26641 gcggtaatag agatcgctac ggaactctcg gtcggcgacc atttttttca gatcgcggtt
    26701 agtcgcggca attaaacgta cgtccgtctg aatgattttg ttgctgccga gacgttcaaa
    26761 ctcctgttcc tgcaatacac gcagcaactt cggctgtaac tccagtggca tatcgcccac
    26821 ttcgtcgagg aacagggagc ttttatccgc cagttcaaaa cgaccaatgc gctgggcgct
    26881 ggcaccggta aaagccccac gctcatgacc aaacagatcg ctttccagca atccggcagg
    26941 catcgccgcg cagttcattt tgaccatgcg gcgattatta cgcccactga gattatggat
    27001 cgcgcgggca atcagctctt tacccgtgcc agtttcaccg aggatcagca cggtactgtc
    27061 actttgcgcc accatttcaa cttgtttaag cacgctgtac atggcttcgc tgcggccaat
    27121 aatctcgcca aattcactat caacattgtt gagctgctcg gtcagggcga ggttttcatc
    27181 aaccagccgt tctttcagac gatggatttc ctgataggcg agggcgttat cgacagcgat
    27241 tgccacacgt tcggcaatct ggcgcagcaa attcagattg gtagtggtaa acactttctc
    27301 ttcgcattgc gccagtttca gcacgcccag catggtgtcg ccagacatca gcggtaacag
    27361 gcacaaggtt tgaatctggt tgccccaggt gtcgaacaac atgcgttcat agggggctaa
    27421 atcgtcccgc tcgtggagat tgatcagcag catctcttta cttttgaaca cgcgttcggt
    27481 gagggttccg gcttcatcga cttcgctctg ttcgtgggcg ggatgttgtt tatcaagata
    27541 gtgagtggag tagatgttga gtttgttttt acggtggctg cgtaagacga tactgatatc
    27601 gtcaatgtcg aaatagtaat ggatttcttt ggcgacttcg ctgaccagtt cgtccatatc
    27661 caggcgggaa agcaccgcgt tggtaatggc aaccaggatg cggaagttat cgcgctcccg
    27721 gcataacaac tcatagtcga cattgttgac gacgcggctc tggatttgtt cggtgacgac
    27781 agaaacgatc tgcgtaaatg tttgcagacg attgaactct ttttcgctcc agggacgatc
    27841 gtcataacga ataaattcac agccaccaaa aatatgccct tccgccgcca gtggcatcag
    27901 gcaatagtga ccaaatttgg ggtatagccc accagcgacc agctgcggcc aggtttcaca
    27961 aaattcttcg taactgcaat gcagcgtatc agggcgcgac aaaatgcggc gtaccggacc
    28021 gtgtgccaga acagtttcgt cttcatattt aatgggggtg tctttttcac gcgatgcgta
    28081 ataagacgca cgttgagtct gcgcttgcca caacacaata gccgcgttgt cggcgagcgc
    28141 agaacgcttt accagttgcg aaagagcctc acacagcgag gccagatcgg gctgctgcaa
    28201 tagtgtccga gtgatgtcga acaacccttg ctgtccgaga tcactcatcg gtgtatatga
    28261 cattttgttc ttccaggaaa gcaccgcaaa gcggtgcaaa aggtcattga ctaactatag
    28321 gagatttaga attttattag catatacgcg gaagcggttc ggcgtgtggt aaatcgaggg
    28381 ttcgtttcac accatacaga ccggcaagac gaacaccttt acgttccacc acttcaccaa
    28441 ttagcgccgc gtctttcccc agtggatggg aatgtaacgc tgccagcact tgctctgctg
    28501 cgttgcgttc aacagctatt actagtttgc cttcgttggc aaagttaagg gcgtccagtc
    28561 ccagcagttc gcaaacgccg cgcacagcag gtttaaccgg cagtgctgat tcagaaattt
    28621 caataccaca accgcaggct gccgcgaact catgaaccac cgcgtttaca ccaccacggg
    28681 tggcatcacg cagcgctttc acgccgggaa tgtcacgcag cgtctgaata agcggcgtca
    28741 gcaccgcgca gtcgctgacc agttcaccat ccagccccag ttgttcacgc aggttaagga
    28801 tagtcgcccc gtggtcgccg agtgtaccgc taaccagcaa tacatcgcct gcggttagcg
    28861 tctgtgcgcc ccagtgaata ttcgccggaa ttgcgcccat gccagcggtg ttgataaaca
    28921 gtttatctgc cgcgccgcgc tgcaccactt tagtatcgcc agtaacgatg gcaatgcctg
    28981 ccgtgcgggc ggtttctgcc atgctggtca ctacggcttt cagtgtctcc atcggcaatc
    29041 cttcttcgag gataaagcca caggagagat agcacggaat agcgccactg accgcaacgt
    29101 cattcgcggt gccgcaaatc gccagcttgc cgatattacc gccaggaaag aacagcgggt
    29161 caatgacgta actgtcggtg gagaacgcca gacgatcgcc ttctgctacc agctgcgcca
    29221 gatcaagacg tgcctggtct tcctgctctg ccagccacgg gttggcaaag gcttccataa
    29281 acaggctgtt gattaattgc tgcatcgcct ggccaccgct gccgtgggcg agttggatat
    29341 tattcacgct tcactctcct gctgacgata ctgataccac gcggcgcacg ctccttcgga
    29401 ggagaccatc agtgcaccaa atgcggtttg aggattacag gtgttaccaa acagcgggca
    29461 ttgatgcggc ttacatttgc ccgttaatac ctcaccacaa cgcgcgcgcg ggtcatcgca
    29521 gacctgctgc ggtgccgggc ggaaatgtgc ttcggcatcg aatcgttgat aatccggcgt
    29581 caggtgcacg ccagaagatt caatcacgcc taagccgcgc cattcgctgt cgccgttgac
    29641 acagaacaca tcggcaatcg cctgttgcgc cagcaggtta ccggcatccg gtaccacccg
    29701 acgatactga ttctctacct tgctgtgggc cgctattttc tgctccacca gcatcaccac
    29761 gccttgcagt agatcaaggg gttcgaaacc agcgaccacc agcggacgat gaaaatcgct
    29821 ggcgataaaa ttataggcat cagtgccgat aaccatacta acgtggcccg gcgcgaggaa
    29881 cgcgtcgata ccgttatccg gctgttccag caaactgcgc agcgtcggga taagcgtaat
    29941 gtgctggcag aagaagtaaa aattctgcac atcacgcgct ttcgcctgtt gcagagtgat
    30001 ggcggtggtc ggcatggtgg tttcaaaacc taagccgaag aacaccactt tgcgggttgg
    30061 attctcctgc gccagtttca acgcatccat cggcgagtaa acgatgcgca catcggcacc
    30121 gcgtgctttt gcctgcaaca gcgatccctg tttccctggt acgcgcatgg catcgccaaa
    30181 ggtacagaag atgacttccg gatggctggc aatctccacg caggtgtcga ttctacccat
    30241 cggcagtacg cacacagggc aacccggacc gtggataaac tcaacgtttt caggcagtaa
    30301 ctggtcgagg ccgaatttaa aaatagcgtg ggtatgacca ccgcacactt ccataatccg
    30361 cagagggcgt tcggcggtgt aagagagatg tgaagcacgt tcgcgcagat gctcaattaa
    30421 ctgcatcacc tgttccggcg cgcgatattc atcaacaaaa cgcattattt ttcctcgcca
    30481 tacaacagcg cgccgacatc cggctcaacg tcaaacatgt tttgtaaggc gtcgagagtg
    30541 tcgcgtgctt cggcttcagt aattacgctc atggcaaagc caacgtgtac cagtacccac
    30601 tggcccacgc gcggctgacc gttttcatcg cagctgccga ctaacgttaa atcgacatcg
    30661 cgctgaatgc cgcagacgtc gacttttgcc tggttgccgt caatggtgcg gatctggccg
    30721 ggaacgccta tgcacatcgc tgtgtctcca gccagttcag ccactggtcc atcccttcac
    30781 cgctggtggc ggaaataagg atgatttcaa tttctggatt gacttcgcgg gcgcaggcga
    30841 tgcacttctc aacgtcaaag ttgagatacg gcaacaggtc aactttgttg agcagcatca
    30901 gcgaggcggc ggcaaacata tgcggatatt tcagcggttt gtcttcacct tcggtaacgg
    30961 aaagcaccgc cactttgtgt ttttcaccga gatcgaagct ggccgggcat acgaggttgc
    31021 caacgttttc gataaacaga ataccgttat cgtccagtgg cagacgcggt gcggcgtcgg
    31081 caatcatctg tgcgtcaaga tggcagcctt tacctgtgtt cacctgaatc gctggtgtgc
    31141 cggtagcgcg aatgcgtgcg gcatcgttca cggtttgctg gtcaccttca ataaccgcgc
    31201 acggaacgct gtctttcagg cgcattaggg tttccgtcag cagggtggtt ttaccggaac
    31261 ctgggctgga aaccaggttg agcaccagtt gcttgcgggc agcaaagcgc gcgcggttgc
    31321 gttcagccag acggttgttt ttgtccagca cgtcaatttc gacttccagc atccgacgct
    31381 ggctcatgcc cggtgcgtga gtgcccgctt cgccatgacc gtaatgcagg tcgccttctt
    31441 cagtctggct gggggtaaat tcaggcgctt taatgccggt gattttcatc ttcgggcgtg
    31501 ccgccggggc aaatggcgcg ctacgaaacg cggaatgagg gttatgttca tcaccctcga
    31561 tatacaggtt gccttcaccg caaccgcatg ttgtacacat cgctcactcc tggtctattt
    31621 ctatccgccg aatctgtaaa ccgtcgtctg ccacaatctg tagcatgtca ccatgacact
    31681 gtggacagcg gcggacgcgc tgggtcaata gcgtcacata ctgttggcat gtttcacacc
    31741 agcattcgac ctcttgttct tcgaggtgca gtttacaacc ttccgccacg ctgccgcggc
    31801 aaaccagatc aaaacaaaag gcaagagagc tggtttcgac acaagaaaat gcgccaattt
    31861 tgagccagac cccagttacg cgttttgcgc cgtgttttgc ggcctgctgt tcgatcaatt
    31921 ccagtgcccg ttggcagagg gttatttcgt gcatatcgcc tcccattaac tattgccagc
    31981 tacaagcaat aattgtgcca gtgttgatta tccctgcggt gaataatgtc gatgatgtcg
    32041 aaatgacacg tcgacacggc gacgaaattc atctttatct taaaaatcgt tttaataaca
    32101 ataaattaaa aattggcaca gaaaatgctt aaagctggca tctctgttaa acgggtaacc
    32161 tgacaatgac tatttgggaa ataagcgaga aagccgatta catcgcacag cggcatcgtc
    32221 gcctacagga ccagtggcac atctactgca attcgctggt tcaggggatc acgttatcga
    32281 aagcgcgcct gcatcacgcc atgagctgcg cgccggacaa agaactctgt ttcgtccttt
    32341 ttgaacattt tcgtatttac gtcaccctgg cggatggctt taacagccac accatcgagt
    32401 attacgtcga aacaaaagag ggcaaactgt ggtttgcgct ggggcgtctt ggcactctgc
    32461 tgctggcgat tgcttgctgg ctgctgtggc agcgttatgg cacgctggat ctgcgcctgc
    32521 tggatatgcg tatgcaacag ctgccgctcg gttccgatat ctggctgctc ggtgtgattg
    32581 gctttggcct gctcgccggg attattccgc tgcacggctg ggtgccgcag gcacatgcta
    32641 acgcctctgc gccagctgcc gcgttgtttt ctacggtagt catgaaaatt ggcctgctgg
    32701 gcattttaac cctgtcactg ctgggcggta atgcaccgct gtggtggggg atcgtgctgc
    32761 tggtgctcgg catgatcacc gcgtttgtcg gtggtctgta tgcgctgatg gagcacaaca
    32821 tccagcgcct gctggcttac cacaccctgg aaaatatcgg cattatcctg ctggggctgg
    32881 gcgctggcgt aacgggtatc gcgctcgaac aaccggcgct gattgctctt ggcctggtcg
    32941 gtggtctgta ccatctgctt aaccatagcc tgttcaaaag cgtactgttc ctcggggcgg
    33001 ggagcgtctg gttccgtacc ggtcatcgcg atatcgaaaa actcggtggt attggcaaga
    33061 aaatgccggt tatctccatc gccatgttag tcgggttgat ggcaatggct gcgctgccgc
    33121 cgctgaacgg gtttgccggg gaatgggtta tctatcaatc ctttttcaaa ctgagcaata
    33181 gtggcgcgtt tgttgcccgt ctgctggggc cgctgctcgc tgtgggtctg gcaattaccg
    33241 gtgcgctggc ggtgatgtgt atggcgaaag tctatggcgt cacgttcctc ggcgcgccgc
    33301 gtaccaaaga agccgaaaac gccacctgtg cgccgctcct gatgagcgta agcgtagtgg
    33361 cactggcgat ttgctgcgta attggcggtg ttgctgcgcc gtggctgctg ccgatgctct
    33421 ctgctgccgt acctctgcct ctggagcctg ctaacaccac cgtttctcaa ccaatgatca
    33481 cattgctgct gattgcctgc ccgctgctgc cattcatcat tatggcgatt tgcaaaggcg
    33541 accgcctgcc gtcacgttcc cgcggtgcgg cctgggtgtg cggctacgac catgaaaaat
    33601 caatggtgat taccgctcac ggttttgcca tgccggttaa acaggcgttt gcgccagtgc
    33661 tgaaactgcg caaatggctg aatccggtgt cgctggtgcc gggctggcag tgcgaaggta
    33721 gtgcgttgct gttccgccgg atggcgctgg ttgaactggc ggtactggtg gtgattattg
    33781 tttcacgagg agcctgagaa tgagtgtttt atatccgtta attcaggcgc tggtgttatt
    33841 tgccgttgcg ccgctgctct ccggtattac ccgcgtggcg cgcgcccgtc tgcataaccg
    33901 tcgcgggccg ggcgtgttgc aggagtatcg cgacattatc aaactgctgg gccgccagag
    33961 cgttggcccg gatgcctctg gctgggtgtt ccgcctgacg ccgtatgtga tggtgggcgt
    34021 catgctgact atcgctactg cgctgccggt ggtgaccgtc ggttctccgc tgccgcaact
    34081 gggcgatttg atcaccttac tgtatctctt cgccatcgcg cgtttcttct ttgccatttc
    34141 cggtctggat agcggtagcc cgtttaccgc tatcggtgcg agccgtgaag cgatgcttgg
    34201 cgtgctggtc gaaccgatgt tgctgcttgg tctgtgggtt gccgcacagg ttgccggttc
    34261 caccaacatc agcaacatta ccgacaccgt ttatcactgg ccgctgagcc aaagcatccc
    34321 gctggtgctg gcgctctgcg cctgtgcgtt cgccactttt atcgaaatgg gcaaactgcc
    34381 gttcgacctg gcggaagccg agcaggagtt gcaggaaggt ccgctctctg agtatagcgg
    34441 cagcggcttt ggcgtcatga aatggggtat cagcctgaaa cagctggtgg tgttgcagat
    34501 gttcgtcggg gtgtttattc cgtggggaca aatggaaacc ttcaccgccg gtggactgct
    34561 gctggcgctg gtgattgcga tcgtaaaact ggtggtcggc gtcctggtta tcgcgctgtt
    34621 cgaaaacagc atggcccgtc tgcgtcttga tattactccg cgcattacct gggctgggtt
    34681 tggctttgca tttttagcgt tcgtctcctt gctggcggcg tgattaaaga gagtttgagc
    34741 atgtctgaag aaaaattagg tcaacattat ctcgccgcgc tgaatgaggc atttccgggc
    34801 gtcgtgctgg accacgcctg gcagaccaaa gatcagctga ctgtcaccgt aaaggtgaac
    34861 tatctgccgg aagtggtgga gtttctctac tacaagcagg ggggctggct gtcggtgctt
    34921 tttggtaacg acgaacgcaa actgaatggt cattacgccg tttattacgt gctgtcgatg
    34981 gagaagggga ctaagtgctg ggtaacggtt cgcgtcgaag ttgatgccaa caaaccggag
    35041 tatccgtccg tgacgccgcg cgttccggcg gcggtgtggg gcgagcgcga agtgcgcgat
    35101 atgtacggtt tgattccggt tggtctgccg gatgaacgcc gtctggtgct gccggatgac
    35161 tggccggatg aactttatcc gctgcgtaaa gacagcatgg attatcgtca gcgtccggca
    35221 ccgaccaccg atgctgaaac ctacgagttc atcaacgaac tgggcgacaa gaaaaacaac
    35281 gtcgtgccga ttggtccgct gcacgtcact tctgacgaac cgggccactt ccgtctgttc
    35341 gtcgatggcg aaaacattat cgacgcagac taccgcttgt tctatgtcca tcgcggtatg
    35401 gaaaaactgg cggaaacccg catgggttat aacgaagtga ccttcctctc tgaccgtgtg
    35461 tgcgggatct gcggcttcgc ccacagcacc gcctacacca cgtcggtgga aaacgcgatg
    35521 ggtattcagg tgccagaacg tgcgcagatg atccgcgcca ttctgctgga ggtggaacgc
    35581 ctgcactcgc atctgctcaa cctcggcctc gcctgccact ttaccggctt cgactccggc
    35641 tttatgcaat tcttccgcgt gcgtgaaacc tccatgaaaa tggccgagat cctcaccggt
    35701 gcgcgtaaaa cctacggcct gaacctgatc ggcgggattc gtcgcgatct gttgaaagat
    35761 gacatgatcc agacccgcca gctggcgcaa cagatgcgtc gtgaagtgca ggagctggtg
    35821 gatgtgctgc tgagcacacc gaacatggaa cagcgcaccg tcggcattgg tcgtctggac
    35881 ccggaaattg cccgcgattt cagtaacgtt ggcccgatgg tccgcgccag cggacacgct
    35941 cgcgataccc gcgctgatca cccgtttgtt ggttatggcc tgctgccaat ggaagtccac
    36001 agcgagcagg gctgcgacgt tatttcgcgt ctgaaagtgc gtatcaacga agtctatacc
    36061 gcgctgaaca tgatcgacta cggtctggat aacctgccgg gtggcccgct gatggtggaa
    36121 ggctttacct acattccgca ccgtttcgcg ctgggctttg ccgaagcgcc gcgcggtgat
    36181 gatatccact ggagcatgac tggcgacaac cagaagctgt accgctggcg ctgccgtgcc
    36241 gcgacctacg cgaactggcc gaccctgcgc tacatgctgc gtggcaacac tgtttccgat
    36301 gcgccgctga ttatcggtag cctggaccct tgctactcct gtaccgaccg catgactgtg
    36361 gtcgatgtac gtaagaagaa gagcaaagtg gtgccgtaca aagaactcga gcgctacagc
    36421 attgagcgta aaaactcgcc gctgaaataa ggaatcgcca tgtttacctt tatcaaaaaa
    36481 gttatcaaaa ccggtacggc gacctcgtct tatccgctgg agccgattgc ggttgataaa
    36541 aacttccgtg gtaagccaga gcagaacccg cagcagtgca tcggctgcgc ggcctgcgtc
    36601 aatgcctgcc cgtcaaacgc cttaacggtt gaaactgacc tcgccacagg agagctggcc
    36661 tgggagttta atctcgggcg ctgcatcttc tgtggacgct gcgaagaagt ttgcccgacg
    36721 gcggcgatta agctgtcgca agagtacgaa ctggcggtgt ggaagaaaga agacttcctg
    36781 caacagtctc gcttcgcgct gtgcaactgt cgcgtctgca atcgtccttt cgccgtccag
    36841 aaagagatcg actacgccat tgcgctgctt aagcacaacg gcgacagccg cgcggaaaac
    36901 caccgcgaaa gctttgagac ttgcccggaa tgtaagcgcc agaaatgcct ggtgccgtcc
    36961 gaccgtattg aactgactcg ccatatgaaa gaggccatct gatgagcaat ttattaggcc
    37021 cccgtgacgc caacggcatt ccggtcccga tgacggtgga tgaatccatc gccagcatga
    37081 aggcgtcgtt actgaaaaaa atcaaacgtt ctgcctatgt ttaccgcgtg gactgcggcg
    37141 gctgcaacgg ctgcgaaatc gaaattttcg ccactttatc gccgctgttt gatgcagaac
    37201 gcttcggcat taaagtcgtt ccttcaccgc gtcatgcgga tattttactg tttaccggcg
    37261 cggtcacccg tgcaatgcga tcccctgcgc tgcgtgcgtg gcagtccgcg ccggacccga
    37321 aaatttgtat ctcctacggt gcctgcggta acagcggcgg gatcttccac gatctctact
    37381 gcgtgtgggg cggtacggat aaaattgtcc ctgtggatgt ttatatccct ggctgcccgc
    37441 caacgcctgc cgccacgctg tacggctttg caatggcgct cggcctgctg gagcagaaaa
    37501 ttcacgcccg tgggccgggt gaactggatg aacaaccggc ggagatcctg catggcgata
    37561 tggtgcagcc gctgcgcgtg aaagtggatc gcgaagcacg ccgcctggcg ggttatcgtt
    37621 acggtcgtca gattgccgat gacttcctga cacagttagg gcagggcgaa gaacaggttg
    37681 cacgctggct ggaagcggaa aacgatcccc gtctgaacga gattgtcagc catctgaatc
    37741 atgttgtaga agaggtgcgt atccgatgag tgaaaaggtg gtgttcagtc aactgagccg
    37801 taaatttatt gatgagaacg atgccacgcc cgccgaggcg cagcaggtgg tctattacag
    37861 cctggcgatt ggtcaccacc ttggggttat cgattgcctg gaagcggcgc tcacctgccc
    37921 gtgggatgaa tatctggcat ggattgccac tctggaggcg ggcagtgaag cccgccgcaa
    37981 aatggaaggc gtaccgaagt acggtgagat cgtcatcgac attaaccatg tgccgatgct
    38041 ggccaacgca ttcgataaag cccgggcagc gcaaacttcg cagcagcagg aatggagtac
    38101 aatgctgtta agtatgctgc atgatattca tcaggaaaac gccatctatt tgatggtgag
    38161 gagattgcgt gactgacgtt ttactctgtg ttggcaatag catgatgggc gatgatggcg
    38221 caggtccgct gctggcggaa aagtgcgccg ccgcgccgaa aggtaactgg gtggtgattg
    38281 acggcggtag tgcaccggaa aacgacatcg tcgccatccg tgaactgcgc cctacgcggt
    38341 tactgattgt cgacgccacg gatatggggc taaaccccgg cgagatccgc atcatcgacc
    38401 cggatgatat cgccgagatg tttatgatga ctacccataa catgcctctg aactatctta
    38461 tcgaccagtt gaaagaagat attggcgaag tgattttcct cggcatacag ccggatatcg
    38521 tcggctttta ctacccgatg acccagccga ttaaagatgc ggtagaaacc gtttatcaac
    38581 gactggaagg ctgggaaggg aatggcggct tcgcgcagtt agcggtggaa gaagagtagt
    38641 ttttcattaa ggaatcagga cagggatgtt cttgatgggg tgaaccagct ctgatgccaa
    38701 atgctaaatt gcccgatgcg ctgcgcttat cgggccttcc tggttcgtgc gacatgtagg
    38761 ccggataagg cgttcacgcc gcatccggca ctgttactta ctctaaatct tccccattac
    38821 tggcaatcac ttttttatac caccagaacg atttcttacg tgtgcgcgtc agcgtgccgt
    38881 tgcctgcgtc gtcacggtct acgtagacaa aaccgtagcg tttgctcatt tcacccgtag
    38941 aggcggaaac taaatcaata cagccccatg tggtgtagcc catcagcgga atgccatctg
    39001 caatcgcttc gcccattgcg cggatatgtt cgcgtaaata gctgatgcga tagtcatcgt
    39061 taatctcgcc attggcagca agttcatctt ttgcgccgag gccgttttcc accagaaaca
    39121 gcggcttctg ataacgatcg tacatcatat tcatggtgat acgcagaccg agtggatcaa
    39181 ttccccagcc ccagtcgctc acttgcagat acgggttacg cagcgatttc accacgttcg
    39241 ccgcactgct gttgttggcg ttcatctccg ccgaggcgca gcgcgaggcg taatagctga
    39301 aagagacaaa atcgacggtg tttttcagga tctcatcatc gcccagcgct ttatctatgg
    39361 ttaccccttt ttcgcgaaat acgcgggcag agtaagccgg atacgcgccg cgcgcctgca
    39421 catcgataaa aaacaggttt tcccgatctt tctccagcgc cgcccagaca tcttccggtt
    39481 tgcagctgta agggtagaag ttgccgcccg ccagcataca gcccacctga ttttgcgggt
    39541 taacctcatg ggcgattttg gcggctagcg cactggcaac cagctggtga tgcgcggcct
    39601 gatatttcac ctgatcctga ttttcacctt cttcaaacac cagacccgcg ccggagaacg
    39661 ggctatgcaa cataatgttg atttcattga aggttagcca gtatttcacc agaccatcaa
    39721 atgcttcaaa gcaggttctg gcgtagcggc tgaaaaactc caccagcttg cggttacgcc
    39781 aggagccata ttcggtgacc agatgcatcg gcacatcgaa gtggcacaac gtgaccagcg
    39841 gttcgatacc gtacttttta cactcttcaa agacagaacg ataaaaagca atgccctgct
    39901 gattgggcgt gatttcatcg ccctgcggaa agagacggct ccaggcaatt gaggtacgga
    39961 aaaccttgaa tcccatctct gccatcaggg cgatatcttc tttataacga tgataaaaat
    40021 ccgtcgcctc atggctggga taaaactcgt catcgcgcaa ctgaaaacgt ttttccagcc
    40081 ccagtttcac cgccattcga tgctcgccgt gtgggatcat atcgacggtg gtcagccctt
    40141 ttccgccttc acggaacgca ccttcagact ggttggcggc aagcgcgccg ccccataaaa
    40201 aaccttctgg aaatactgac attttcatcc tcaattaaga cttacttctt tggcgacggt
    40261 cggttgtacg ctctgatgct ttcgcgcctg ggcagccgct tcctcaacag gaatatcctc
    40321 aaagccgagc aacagtgtga ggataaacga cagcaccacc gccagcgcca tgaccgcgaa
    40381 cacccagacg atgctcattg gattcgccgg atcgaagaat tgcacgctgg taaatagccc
    40441 cggcgctgcc attgagtggc tggcaagccc cgccataccg gcaaccgcgc cgcaaataaa
    40501 accgctgata agactggcga taagcggacg tttcaggcgg atcgccacgc cgtataacgc
    40561 cggttcggaa atccccgcca taatggctga tgccgccgca gccagcgccg tctggcgcag
    40621 ttccgggttt ttcgttttcc acgccaccgc cagtgatgaa ccgcccagcg acaggttagc
    40681 gccgatctct gacggcatga ccatcccttc tttgccggtt tcggcaatgg tctgaatgat
    40741 ggttggcgta aagacgcggt gcatcccggt cattaccagc agaggccaca gcgcgcccat
    40801 aatggcgact gaaagccagc ccagataacc atgaatgctg taaaccagcg ccgaaatggc
    40861 gctaccgatc cagataccaa tcgggccaat cagcaggatt gccagcggtg cggcaatcaa
    40921 cacaatcagc attggcttga ggaagttttt ggtcaccgcc ggtgtaatgc gatccaccca
    40981 gcgttcgata tatgacaggc accaggtcat caccagcgcc gggatcactg tgtaggtgta
    41041 tttcaccgcg gtgaccggaa tcagggcaaa ttcgacatgt tcaccctggg ccgctttcgc
    41101 catcagttca ataaaactcg gatgtaccag cacacccgcg atggcaatcg ccagcgacat
    41161 attggtttta aatttgatgt cggcagatgc cgcgaccatc agcggcagga agaagaaagc
    41221 accgtcacca atcacattca gaatggttaa ggtcggcgat ccttttgtca gcacgccgct
    41281 catctcgagg atcatcgcca gcagtttgac catcgatccg ccgataatcg ccgggatcag
    41341 cggtgacatg gtgccgatca gcgcatcgag gatccccgca ccaatgcgac gcggcgtgag
    41401 tttgggttta cccacgggct gtgcgggctg catatctccc ggcagcaggc tgacgacttc
    41461 ctgaaaggct tgtgaaacgg tattgccgat aatcacctga cactggttgt cactacgtac
    41521 cacgccgagc acgccgggga tggtttttaa cgtcgggctg tcgataagct ggtcatcttt
    41581 gataacaaag cgcaaacgcg tcatacagtg cgtgaccgcc gagatgttat caacgccgcc
    41641 cagtgccgct atctccgagc gtgccagcgc cgcataattt ttggccatcg gattttatcc
    41701 tgttatcagt agggtagatg tagttgttat cagtcgacgt aacggaaaaa tgccgatgct
    41761 gaaacggaat taggaaaccg gtttcacaaa atcatgaata gttgtcacta aataagcaag
    41821 agagttaatt attcattttt tatatttgtg aaaccggtca cctgaatatt tttcccgccg
    41881 attgcgcaag gacaatcggc aaacggtgca gtacactgcg ttgattgcgt gtaaagggat
    41941 gaaaatgatg acgacgatgc tggaagtggc gaagcgcgcc ggggtttcaa aagcgaccgt
    42001 ttcccgcgtg ctttcaggta atggctacgt cagccaggag actaaagatc gcgtgtttca
    42061 ggcggtagaa gagagcggtt accgcccaaa cttgctggcg cgcaatctgt cggcgaagag
    42121 tactcagacg ctggggctgg tagtgaccaa cacgctttac cacggcattt attttagtga
    42181 attactcttt catgccgcgc gaatggcgga agagaaaggg cggcagttgc tattggcaga
    42241 tggtaaacac agcgccgaag aagagcgcca ggcgattcag tatctgctgg atttgcgctg
    42301 caacgccatt atgatttacc cgcgtttttt aagcgtggat gagatcgatg acatcattga
    42361 cgcgcacagt cagccgataa tggtgcttaa tcgccgcctg cgcaaaaaca gcagccatag
    42421 cgtctggtgc gattataaac agaccagctt taatgccgtg gcagagttga taaacgccgg
    42481 acatcaggag attgctttcc ttaccggctc gatggattcc cccaccagca ttgaacgtct
    42541 tgctgggtat aaagacgcgc tggcgcagca tggtattgcg ctcaatgaaa aacttatcgc
    42601 taacggtaaa tggacgcccg ccagcggggc cgaaggggta gaaacgttgc tcgaacgtgg
    42661 ggctaaattt agcgcgttag ttgccagtaa cgacgatatg gcgataggtg cgatgaaagc
    42721 gttgcacgag cgcggcgtag cggtgccaga gcaggtgtca gttatcggat tcgatgatat
    42781 cgcaattgcc ccctatatcg ttccggcgct ctccagcgta aaaattccgg tgactgagat
    42841 gattcaggaa attattggac ggctgatttt tatgctcgat ggcggggatt tctcaccgcc
    42901 gaaaaccttc agcggaaaac tgatccaccg cgactcactc attgctcttt cgcgataaac
    42961 attgtcatca catgtcatcg tcactagtgt cgatgacatc ttttttcctg cattacagaa
    43021 attaatttcc ttaaataaca gtgaattaaa aactggcatg atttgtgaat gtatcggcgc
    43081 attaactgtc attgctggag aatttgatga accgtttcat cattgctgac gcgagtaaat
    43141 gtattggttg ccgtacctgt gaagtggcgt gcgtggtttc tcatcaggaa aatcaggact
    43201 gtgcatcgct gaccccggaa acatttttac cgcgtatcca tgtcattaaa ggtgtgaaca
    43261 tttccacggc gacagtttgc cgtcagtgtg aagatgcacc gtgcgctaac gtctgcccga
    43321 atggtgctat cagccgtgat aaagggtttg ttcatgtgat gcaggaacgt tgcattggtt
    43381 gcaaaacctg cgttgtggct tgcccgtatg gcgcgatgga agtggtggta cgtccggtga
    43441 ttcgcaacag cggcgcgggg ctgaatgtac gggctgacaa agccgaagcc aataaatgtg
    43501 acctgtgcaa ccatcgtgaa gacggcccgg cgtgtatggc ggcttgcccg actcatgcgc
    43561 tgatttgtgt cgatcgtaat aaacttgaac aactgagtgc agaaaaacgc cgccgtacgg
    43621 cgctgatgtt ctaaaaatct tacagaactg ttctggcatc aaaggaattc ccggaaatat
    43681 tcgtagaata cggagcaggc ggtatcgccg acctgtgact gccggatgcg acgctgtcgt
    43741 gtccggctgg gcgggttacg caattcatga tggcgggaat aactcaatgg caaaaaacac
    43801 atcttgcggt gtccaactgc gtattcgtgg caaagtgcag ggcgtcggtt ttcgtccgtt
    43861 tgtctggcag ctggcacagc aattaaatct tcacggcgat gtctgtaatg acggcgatgg
    43921 cgtagaagtc cggctgcggg aagacccgga aacgtttctt gttcaattgc atcagcactg
    43981 cccgccgctg gcgcgtattg atagcgtcga gcgtgagccg tacatctggt cacaactgcc
    44041 cactgagttc accatccgcc agagcgcggg aggtgccatg aatacgcaaa ttgtcccgga
    44101 tgccgctact tgccctgctt gccttgccga aatgaatacc ccaggcgaac ggcgttatcg
    44161 ttatccgttt atcaactgta cccactgcgg cccgcgtttc accattattc gcgccatgcc
    44221 ttacgaccgc ccgtttaccg tgatggcggc gtttccgttg tgtccggctt gtgacaaaga
    44281 gtaccgcgac ccgctcgatc gtcgcttcca cgcccagccg gtggcctgcc cggagtgtgg
    44341 cccgcatctt gaatgggtaa gtcatggtga acatgcagaa caagaggcgg cattacaggc
    44401 ggctatcgca cagttaaaaa tgggcaacat tgtcgccatc aaagggattg gcggatttca
    44461 tcttgcctgc gatgcacgta acagtaacgc ggtggcgaca cttcgggcgc gcaaacatcg
    44521 cccggcgaaa ccgctggcgg tcatgttgcc agtggctgac ggtttaccag acgctgcgcg
    44581 ccagttgctt accacgcctg ccgcgccgat tgtgctggtg gataaaaaat acgttcctga
    44641 gctttgtgat gatatcgccc ctgaccttaa cgaagtcggg gtaatgttgc ctgcgaaccc
    44701 gctccagcat ttgctgttac aggaactgca atgcccgctg gtgatgacct ccggcaacct
    44761 gagcggtaaa ccaccagcta tcagcaacga acaggcgctg gcggatttgc agggcattgc
    44821 cgacggattc ttgatacata accgcgacat cgtgcagcgg atggatgatt cggtggtgcg
    44881 cgaaagcggc gaaatgctgc gccgttcgcg ggggtatgtg ccggatgcgc tggctttgcc
    44941 tccgggcttt aaaaatgttc cgcctgtgct gtgtctcgga gcggatctga aaaatacctt
    45001 ctgcctggtg cgcggtgaac aagcggtgtt gagtcagcat ctgggcgatt taagtgacga
    45061 tggcatccag atgcagtggc gcgaagcgtt acgcctgatg caaaacatct acgattttac
    45121 cccgaaatac gttgtgcatg acgcgcatcc gggctatgtc tccagccagt gggcgcgtga
    45181 aatgaatctg ccgacgcaaa cggtactgca tcatcatgcc catgcagcgg cgtgtctggc
    45241 agagcatcag tggccgctgg atggcggtga tgtcattgct ttgacgctcg acggtatcgg
    45301 tatgggggag aacggcgctt tgtggggcgg cgagtgcctg cgggtgaact atcgcgaatg
    45361 tgagcacctg ggcggcttgc ctgcagtggc gcttccgggt ggcgatttgg cagcgaagca
    45421 gccgtggcga aacctgctgg cgcagtgcct gcgctttgtg ccggagtggc agaattaccc
    45481 tgaaacagca agtgtgcaac agcaaaactg gagcgtgctg gcgcgggcca ttgagcgtgg
    45541 aattaacgcg ccgctggcgt catcgtgtgg gcggttgttc gatgcagtgg cggcggcact
    45601 gggctgtgcg ccagccacgt taagttatga aggtgaagcg gcttgtgctc tggaggcgct
    45661 cgcagcctca tgccacggag tgacgcatcc ggtgacgatg ccgctggtgg acaatcaact
    45721 ggatctcgcc gctttctggc agcagtggct gaactggcag gcacaggtta atcaacgcgc
    45781 gtgggcgttt catgatgcgc tggcgcaggg ttttgccgcg ttgatgcgtg agcaggccac
    45841 gatgcgtggt atcactacgc tggtatttag cggcggggtt attcataacc gtttgctgcg
    45901 tgcacgtctg gcgcattatc tcgctgattt cacattgctc tttccacaga gtttaccggc
    45961 gggtgatggc ggtttgtctc tggggcaggg ggttattgct gcggcgcgtt ggttagcggg
    46021 tgaagtccag aacggataaa acgtgttgca tttggacacc attgccggat gcggcgtaaa
    46081 cgccttatcc ggcctacaca tgcgtccccg taggtcggat aagacgcgct agcgtcgcat
    46141 ccgacatttt aggcacagta gcccacctac atcggcaatg ttttcaacaa tccaaatgcc
    46201 tctttcatcc gatcctcact gaccacaaag gcgcgaagct ggtcagcatc gtcaacaccg
    46261 cgagccacca ttccctagcg ttcggtatta atttgccagc gtaaatcctg acgctgggtt
    46321 tcgcctgcca gatgcagcgg taattccggc gttttgattt tcaccagcat cgtcggtagt
    46381 ttcagcggcg tgttattgcc gagaagattt tttgccagca ccatcgcgct aagttgaatc
    46441 ggctgcagga atggcagtac ctgaccgttg atttccgcgt aatcgcccag cgcataaata
    46501 tcgtcattac tggtttgcag ataactatcg acgcaaacgc cgcgattaat cgtcagcccg
    46561 gcgcgtcgtg ccagggctgt ttccgggcgc agtccggtgg cggcaattac cgcatccact
    46621 tcgatactac gctggtggtc cagcgttgcc agaatgccag aatccgtttt ttccagcccc
    46681 tgtaactgag atttcaacag cagatgaacg cccatctccg tcaaccgatg ctgcaagcgg
    46741 ctgcttactt ccggtggcat taacgacgcc agaatactgg cagcgttgtc gattagcgtg
    46801 accgctttgc ctgcacgaca aaaatccatc gccagttcgc taccaatcaa accaccgcca
    46861 acaatcaaca cgcgtcgggc atcccgcagt tgcgtttcac aggcgcgata ctcttgctga
    46921 ctatttaacg tcagcattaa ctcacgccca ggcacaggcg ggacaaaggc actggcaccg
    46981 gttgccagta ctagcttgtc gtattgccac tgattattct ggcttttcac cacacgggct
    47041 tcggcatcga tatccgtcac ccaggtttgt ggaaacaggt gcagattaaa ctgctcggca
    47101 aattcacccg ccgtctggcg ggtaaggtca tcggcacgtt gcccctgact gataacatgg
    47161 ctgaggtcag gtttgttgta ctcatccatg ctgtcggcgg caatcagggt taatggaata
    47221 gtggcgtcct gtttgcgaat atttttcacc agttggcggg cggcgaagcc cgaaccgatg
    47281 atcacaatgc cgttactcat tttgcctccg atgccagttc ttcaaagacg tctttgccga
    47341 gggagcattc cgggcagagg aagttatccg ggacttcact ccacggcgtt cctggcgcaa
    47401 cgtcctgcat tggctcgcct tttgccggat cgtaaatcca ctggcagacg ctgcactgca
    47461 tccgtgggcc gaggtcagcc gtcgtggtgg cagaggtttc ttctttgact accttattca
    47521 ccgtgctctg cggcagcggc gcgagcgccc actgacgggc gatttcgcga ccgtgttcac
    47581 ggcataactc cagagcgtcc tggtctggtc gccatttcgc tttcaggcta agcgacattt
    47641 cgaaacccgc atcctgcagg cgcgtggaaa gacgatccac cgcaccgccg ctccagccgt
    47701 gagagccgaa agcactggcg cgtttgttac ggaagcgtaa accagtcatc tcctccacca
    47761 gcccggcgat tttcggcatc atcacgttat tcatcgtcga agtgccgacc agcacgcctt
    47821 ttgagcggaa gacattggtc aggatttcgt ttttatcgct tcgggcgacg ttgaaaattt
    47881 tcaccgccac gcgtgggtcg gtttccgcaa tcccctgggc gatagcgtca gccatcatgc
    47941 gggtgttatt cgacatggtg tcgtagaaaa tggtgattct gtcttcctga taatcagccg
    48001 cccatttcag gtacagctcg acaatttgcg tcgggttatc gcgccatacc acgccgtgag
    48061 aggtcgctat catatcgact ggtaagttaa agcccaggat ctcggtaatt ttcggtgtta
    48121 ccaggcggct gaacggcgtc aggatattgg cgtagtaacg ctggcactgc tcgaaaagct
    48181 ccgtctgatc cacttcatcg ttgaacagat gctcgtcgca gtagtgctga ccgaaagcat
    48241 cgttactgaa cagcaccgcg tcgcctgtca ggtaagtcat catgctgtcc ggccagtgca
    48301 gcattggcgt ttcgacaaaa atgagctgtt tgccgttgcc gatatccagc gtgtcaccag
    48361 ttttcaccac attaaaattc cactccggat gatggtgatg accatttatc gagtcgatag
    48421 cgttggcagt acagtaaatc ggcgtatcgg gaatttgcgc catcagttcg gtcagcgccc
    48481 cagcgtggtc ctcttctgca tggttaatca cgatgtaatc gatatccgcc agatcgattt
    48541 cattacgcag gttctgcaca aattcgcggc tgaatttatg gtcgacggtg tcgatcagca
    48601 cgtttttttc ttcgcggatg aggtagctgt tgtaactgct gccgcgcagc gttttatatt
    48661 ccgtaccgtg aaaatcacgc acttcccagt cacgttgacc aacccaatga atgttatttt
    48721 tcaccacaat agacatagca acctcaattt attcagcgtg ttctaaaaag atgtcttgct
    48781 aattgcagat tacgtgccag ttttttatct gattgttttt actctataaa taaaattatg
    48841 ctcattaatg agtagtcaaa atgactatca atcaaatggt cgatatgaca atatctatag
    48901 tcaaattgac agtgaggcaa agatgagttt ttccgttgat gtgctggcga atatcgccat
    48961 cgaattgcag cgtgggattg gtcatcagga tcgttttcag cgcctgatca ccacgctacg
    49021 tcaggtgctg gagtgcgatg cgtctgcgtt gctacgttac gattcgcggc agtttattcc
    49081 gcttgccatc gacggtctgg caaagggtgt actcggtaga cgctttgcgc tggaagggca
    49141 tccacggctg gaagcgattg cccgcgccgg ggatgtggtg cgctttcccg cagacagcga
    49201 attgcccgat ccctatgacg gtttgattcc tgggcaggag agtctgaagg ttcacgcctg
    49261 cgttggtctg ccattgtttg ccgggcaaaa cctgatcggc gcactgacgc tcgacgggat
    49321 gcagcccgat cagttcgatg ttttcagcga cgaagagcta cggctgattg ctgcgctggc
    49381 ggcgggagcg ttaagcaatg cgttgctgat tgaacaactg gaaagccaga atatgatgcc
    49441 aggcgatgcc acgccgtttg aagcggtgaa acagacgcag atgattggct tgtcccctgg
    49501 catgacgcaa ctgaaaaaag agattgagat tgtggcggcg tccgatctca acgtcctgat
    49561 cagcggtgag acgggaaccg gtaaggagct ggtggcgaaa gcgattcatg aagcctcgcc
    49621 acgggcggtg aatccgctgg tctatctcaa ctgtgctgca ctgccggaaa gtgtggcgga
    49681 aagtgagttg ttcgggcatg tgaaaggagc gtttactggc gctatcagta accgcagcgg
    49741 gaagtttgaa atggcggata acggcactct gtttctggat gagatcggcg agttgtcgtt
    49801 ggcattgcag gccaagctgc tgagggtgtt gcagtatggc gatattcagc gcgttggcga
    49861 tgaccgtagt ttgcgggtcg atgtgcgcgt gctggcggcg actaaccgcg acttacgcga
    49921 agaggtgctg gcagggcgat ttcgcgctga cttgtttcat cgcctgagcg tgtttccact
    49981 ttcggtgccg ccgctgcgtg agcggggcga tgatgtcatt ctgctggcgg ggtatttctg
    50041 cgaacagtgt cgtttgcggc tggggctctc ccgcgtggta ttaagtgccg gagcgcgaaa
    50101 tttactgcaa cactatcgtt ttccggggaa cgtgcgcgaa ctggaacatg ctattcatcg
    50161 ggcggtagtg ctggcgagag ccacccgcaa cggcgatgaa gtgattcttg aggcgcaaca
    50221 ttttgctttt cctgaggtga cgttgccgcc gccagaagcg gcggcggtgc ccgttgttaa
    50281 gcaaaacctg cgtgaagcga cagaagcgtt ccagcgtgaa actattcgcc aggcactggc
    50341 acaaaatcat cataactggg ctgcctgcgc gcggatgctg gaaaccgacg tcgccaacct
    50401 gcatcggctg gcgaaacgtc tgggaatgaa ggattaaata atcccggcct gatagaaatc
    50461 ctgcaggtta attgcgccgg tgagtttgcc gttttcatcc accaccggtg cggcagtgat
    50521 tttgcgcttc atcaggatct ctttggcgtc gatggcgcga ctttgcgcct gtaacgtggt
    50581 gccgcctgtc gtcatcgctt cattaactgg cgtggtgagt gcgccgccgc ctaccagcca
    50641 gcgacgtaaa tcgccgtcgg taaagacgcc ttgtacctgt tgttgagcgt cacataccgc
    50701 caccagcccc agaccggtgc ggctgagttc cagcatcgca tccatcacgc tggcggttaa
    50761 cgccacctgt gggatggcat cgtcacggcg catcagatga tgcactttat tcagcaagcg
    50821 agcgcccagt gccccggctg ggtgggagcg ggcaaaatct tcttcattaa atccgcgcgc
    50881 ctgcatgacc gccatcgcca gcgcgtcacc catcatcagg gtattgacgg tgctggaggt
    50941 cggcgcaagg tgcatcgggc aggcttcgcg ttctacggag atatccagca ccgcttttgc
    51001 cgccaggccc agcggtgacg tcggtttgcc ggtcatcgcc agcagcgcga tagatttatc
    51061 ttccagacgc ggaataatca gatccagttc cttcgcgcca ccggagtaag agataaacag
    51121 catcacatcg cggctttcaa tcatccccag atcgccgtgc agtgcttctg ccggatggac
    51181 aaaaaaagcc ggtgtgccgg tactggcaag tgttgcggcg attttcttac caatgtggcc
    51241 cgatttgcca attcccgaaa ccaccacttt gccttcgcag tgcaggatga tattggcggc
    51301 gcgaacaaaa tcatcgccca gacgttccgg taaacggctt gcttcctgca actccagcat
    51361 taacgtctga cgtcccgcgt tcagtagtgc ttcactcatt gctctctccg gttatgatca
    51421 catcgatccc tttctcttcc agcgcctgac gaaacgccgg atcgatacct gcgtcggtaa
    51481 tcagcttatc gacgctttca agactgcaaa ctacgttggg gcttttacgg ccaaactttg
    51541 atgagtccgc catcaaaatc acttcgcgcg cggcattgca cattgcctta ctgacggtat
    51601 aaacctcgtt gaaggtggtt acgcccgcat tgagatcgat gccgtcggtg cccataaaca
    51661 atttatcgaa actgaaatgt tcgaaggcat tctctgccag ctgtccgtga aatgaggccg
    51721 attttttacg aaacgttccg cctggcatca ggatagtttg ttcgttatcc agttcggata
    51781 gcgcattgac gatatgcagg ctgttggtca tcaccgtgat gttattaaag cgcgagagca
    51841 ggggaaccat ctgcaaaacg gtactgccag catcaagaat gatcgaatcg ccatcatgga
    51901 taaaactaac ggcagcttct gcaatcagct ctttcttgtg ggtgttgatg agtgttttat
    51961 gatcgatagg cggatcggat tcctctttat tcaacaccac tccgccataa gtacgaatga
    52021 cggttccggc atgttccaga atgaccagat ctttgcgaat ggttgtgcct gtggtgtcaa
    52081 agtattgcgc caattcttca accgagcatt taccctgctt ttgcagatac tccagaatgg
    52141 cggcctgacg ctgacgaggt ttcatgggcg tgattacctg atttaggttt caaaatgata
    52201 acttcgcaag caagtaacat tcacaacgaa attatccacg tttcagttta agcgccaatg
    52261 atagagcatt ctgtgatagt ggatcatggg gaaagatcac atcgggctgt aaatcacccg
    52321 catgcatacc ggtaattgcg ggaatttttt gcggtcgggc gaagacggtc agtcctttca
    52381 taaacaaggt gtcgacgatg cgctgctgat cgtccagccg atggcgacca cgacccgcgg
    52441 tttaaagcgc ccgctggaac gacccacgcc aacccgccct tgctggcata gtgtgtcgaa
    52501 ggcacggtta aaacgagaaa tttgccagcc gcctaaggcc agttgcgcac accaggcgat
    52561 aacggcgacg gtgatgagtg cggataccat aacttttcct taatgtagtg gcggaagtcc
    52621 tgtatgcctg atgagcgtag cgcatcaggc attctatagt ctcacggaga tccttaatgg
    52681 atctccgctg ttgatcagaa catcacctga ccgccggtga cattgatcga ctgtccggtg
    52741 cagtacgacg ccttaggact ggcgtagaac agcagcatat tcagcacatc ttgataatcg
    52801 cagccgcgtt tgagcggtac tttgtcaatg taatactgct cgacttgatc cggtttgata
    52861 cccagcttgg tcgcgtattg tggcaacagt gactggaaca tcggcgattt cagcaggtta
    52921 ccgagcatca gtgaatgcac cgtaatgccg tactccacca gatccagcgc cagtgattga
    52981 gtcagcccga cgccaccaaa tttcgctgcg ctgtagccag agttgtgttt gctgcccact
    53041 ttgccggatt tcgagttgat ctgaataatg cgaccctgaa tcccgtcgcg gatcatcaaa
    53101 cgcgaaaatt cacgggcaca caggaaataa cccaccagat tcacttgtag cgaacggtca
    53161 aaatcgccga gctggaagtc gctgataaag gctgcttttg ctattccggc gctatagacc
    53221 agcaaatcca cgcgaccaaa gatttcatct accccacgag agagcgccag aacgctttgc
    53281 tcgctagtgg cgtcagcacc aaaaccgtac gccatacttt caccatattc ggcgttaatt
    53341 tcttgtgcca catttgcggc tttgtcgctc tgaatatcga caaccgcgac gcgatacccc
    53401 tcggcaacca gaccgtggca caggaacgcg cctaaggttt gcccaccacc gatgacaacg
    53461 gcaacctgat tcatttttta ctccttaaca gattcaaact tcaaaaccga tcccggcgcg
    53521 atatcgtcgg gtacagggcc tgccacatgg acagtgcccg gaaattccgc ttcgtttaaa
    53581 ccatcgaagc gcagggtgac atgacccagt tcgcgaaggt tgtcttccgc cacgctgcca
    53641 acagcggtca ccggatagcg atgctgcccg agcgtaaatt gcaaaccggg atggagtgca
    53701 cctttcaact cgccgtggca atgaatgaag caatactctt cgaggtccgc tggcgcgcct
    53761 tcacgaaagg tgatgagcat ctggtcgctg agggcgtcag tggcactcgc gccgatacgg
    53821 gtgatggtgg tcttataaat aacggtcatg tttcagcctc tattgataga taaaaccgga
    53881 gacaaaccag gcgatcagta cagtcggtgc gccggttaaa aagcggctca ccagtacaga
    53941 agggacaccg acgcgaaccg tgtcctgacg ggcttccgcc agcgacaaac cgaccgggat
    54001 gaagtcgcag gccgcctgcg cgttgatggc aaacagtgcc ggtaaagcca gatgcggcgg
    54061 aatattgccg agaccaatct gcacgccaat caatacgccg ataacctgtg cgataactgc
    54121 gcctgggccg aggaaaggtg aaagcagtgg gaaggagcag atgagcgcca gcatgaccag
    54181 acccagtgga tggctcgcca gcggagcaag accgtgggca atccagtcac caaggccaga
    54241 agccataatg atgccaatga gcgccgagac gaatgccata aacggcagaa tggtttttaa
    54301 tacagtgtcg atggtgtcac gaccagattg aaacagcacc gcaacggcgg accccatgcc
    54361 cattcccacc ttcgccagta aaccatcgct ttgttcggtg attttcttac tggtgtcata
    54421 gtcacggccc acggaagagg gttgtggtgt cgcatcaccc actacagtga tgttttcttc
    54481 ttttacgcca gagacataaa tatcttccac aatgtactgc gccagcggac cggacttgcc
    54541 cgtcgagtgg atattaatgg tgggaatacg tcgtttcgga tagatgccgc agcgtaatgt
    54601 gccgccacag tcgattaccg cgacaccaat ttccgcctcc gcgggttcac cttctttaaa
    54661 tccgtcaata gcctgccagc cagtaagttg tgccagtttg tcaacaatcg caggccgggt
    54721 accggcggtg atatagacga tttttttgcc cggcgtggct tccagctcaa gcgggccgcc
    54781 ccagccaccc gttccttttt cgatccgaat acgcgtcatg atgttgctcc tgccaggtga
    54841 actttttgtt caagttgaat gcccatcttt ttctcaaaaa tggcggtggt cagatcggtc
    54901 acccagccgc gaaagaagtt ggtgaccaga ccaaccagca gatagctcac cgccagtggg
    54961 ccaagtggca ggttcagcgt tgtcagaccg ctggcaatgc caagataaac aaacagttcg
    55021 ccagggttga tatggggaaa gaggccattc attgagtggc agctataaga ggccgccgcg
    55081 tagtagctgg gtttgtactt ttccggcata aagcgaccct ggcttaaggt catcggattg
    55141 caaaagacaa acgtgccaat gcacggtagc agcaggtaac gggaaacagg gttaccggcg
    55201 caacgttgag caaaacgttc aatacgatgc tgaccgataa aattaatcag tgcgttcatg
    55261 ataaccagca ggctaatcaa cagcggaaga atgccggtca ccatcccggt aaacacctct
    55321 ccgccctttt ggaacagccc gataaaccac tctgcaccat gagtaatggt ttctatcatt
    55381 gttctctcct tcaggattta ttgttttatt accaaacggc aacctaatct aatcagattg
    55441 aaagatttaa aagtgttatt ttaatctaaa aatgaaagat aaatatttta atttggaagt
    55501 ttgagtaaaa ggatagcgag gggaatgagt tgagttatgt aaagtccgta tcgggcagtg
    55561 actaccgctt ccttgtgcgg ggcgtgatgc tttaccatac ttgcccctgg ttgaatctgt
    55621 taaatggacc cctcatgttc aagcgtcgtt atgtaacatt gcttcccctt tttgtgttgc
    55681 ttgccgcctg tagcagcaag ccaaaaccta ctgagactga aacgaccacc ggaacgccgt
    55741 ctggcggctt cctgcttgag ccgcagcaca atgtgatgca gatgggcggc gatttcgcta
    55801 ataacccgaa tgcccagcag ttcatcgaca aaatggtgaa caaacacggt ttcgatcgtc
    55861 agcagttgca ggaaattctc tcccaggcga agcgtctgga ttcggtactg cggctgatgg
    55921 ataaccaggc accaaccaca tcggtgaaac cgccatcagg tccgaacggc gcatggctgc
    55981 gttatcgcaa aaaatttatt acgccggaca acgtgcagaa cggtgtggtt ttctggaatc
    56041 agtatgaaga tgcgttgaat cgcgcgtggc aggtgtatgg cgtaccgccg gaaattatcg
    56101 tcgggattat cggcgttgaa acccgctggg ggcgcgtgat ggggaaaact cgcatcctcg
    56161 atgcgctggc aacgctgtca tttaactacc cacgccgcgc ggagtatttc tctggcgagc
    56221 tggaaacctt cctgctgatg gcgcgcgacg agcaggacga tccgctcaat ctgaaaggtt
    56281 cctttgccgg ggcgatgggc tacggacagt ttatgccgtc gtcttacaaa caatatgcgg
    56341 tagatttcag cggcgacggg catatcaacc tgtgggatcc ggttgatgcg atcggtagcg
    56401 tggcgaacta tttcaaagcg cacggctggg tgaaaggcga tcaggtcgcg gtaatggcaa
    56461 acggtcaggc tccaggcttg ccaaatggct tcaaaaccag gtacagcatt tcgcagcttg
    56521 ccacggcagg tttaacgcca cagcagccgc tgggcaacca tcaacaagcc agcctgctgc
    56581 gtctggatgt cggtaccggt taccagtact ggtacggtct gccgaacttc tacaccatta
    56641 cccgttataa ccacagcacc cattacgcaa tggcggtctg gcagttagga caagccgtgg
    56701 cgctggcgcg cgtgcagtaa ctatttgcaa gaggggggcg tgagcttccc tctggctaat
    56761 cagctttttt ctgaatcctc ctcgtaaaat tgcaacgcca acaccatctt cctgacgaaa
    56821 gtgctatctt gtccggcata aatttttgac tgacagaggt tgtgatgact gacagtgaac
    56881 tgatgcagtt aagtgaacag gttgggcagg cgctgaaagc ccgtggcgca accgtaacaa
    56941 ctgccgagtc ttgtaccggt ggttgggtag cgaaagtgat taccgatatt gccggtagct
    57001 ccgcctggtt tgaacgcgga tttgtcacct acagtaacga agccaaagcg cagatgatcg
    57061 gcgtacgcga agagacgctg gcgcagcatg gcgcggtgag tgaacccgtc gtggtggaaa
    57121 tggtgatagg cgcactgaaa gcggctcgtg ctgattatgc cgtgtctatt agtggtatcg
    57181 ccgggccgga tggcggcagt gaagagaagc ctgtcggcac cgtctggttt gcttttgcca
    57241 ctgcccgcgg tgaaggcatt acccggcggg aatgcttcag cggcgaccgt gatgcggtgc
    57301 gtcgtcaggc tactgcgtat gcattgcaga ccttgtggca acaatttcta caaaacactt
    57361 gatactgtat gaacatacag tataattact tcaacagaac atattgacta tccggtatta
    57421 cccggcatga caggagtaaa aatggctatc gacgaaaaca aacagaaagc gttggcggca
    57481 gcactgggcc agattgagaa acaatttggt aaaggctcca tcatgcgcct gggtgaagac
    57541 cgttccatgg atgtggaaac catctctacc ggttcgcttt cactggatat cgcgcttggg
    57601 gcaggtggtc tgccgatggg ccgtatcgtc gaaatctacg gaccggaatc ttccggtaaa
    57661 accacgctga cgctgcaggt gatcgccgca gcgcagcgtg aaggtaaaac ctgtgcgttt
    57721 atcgatgctg aacacgcgct ggacccaatc tacgcacgta aactgggcgt cgatatcgac
    57781 aacctgctgt gctcccagcc ggacaccggc gagcaggcac tggaaatctg tgacgccctg
    57841 gcgcgttctg gtgcagtaga cgttatcgtc gttgactccg tggcggcact gacgccgaaa
    57901 gcggaaatcg aaggcgaaat cggcgactct cacatgggcc ttgcggcacg tatgatgagc
    57961 caggcgatgc gtaagctggc gggtaacctg aagcagtcca acacgctgct gatcttcatc
    58021 aaccagatcc gtatgaaaat tggtgtgatg ttcggtaacc cggaaaccac taccggtggt
    58081 aacgcgctga aattctacgc ctctgttcgt ctcgacatcc gtcgtatcgg cgcggtgaaa
    58141 gagggcgaaa acgtggtggg tagcgaaacc cgcgtgaaag tggtgaagaa caaaatcgct
    58201 gcaccgttta aacaggctga atttcagatc ctctacggcg aaggtatcaa cttctacggc
    58261 gaactggttg acctgggcgt aaaagagaag ctgatcgaga aagcaggcgc gtggtacagc
    58321 tacaaaggtg agaagatcgg tcagggtaaa gcgaatgcaa ctgcctggct gaaagataac
    58381 ccggaaaccg cgaaagagat cgagaagaaa gtacgtgagt tgctgctgag caacccgaac
    58441 tcaacgccgg atttctctgt agatgacagc gaaggcgtag cagaaactaa cgaagatttt
    58501 taatcgttcg ttattgatga taatagataa tgtgtgcacg gacggtcccc tcgccccttt
    58561 ggggagaggg ttagggtgag gggaacaggc cagcactggc gtgaaaaatt actctcacct
    58621 cgatcctcta acgaattggg cctgcgggaa cattctgcac actcattgac tattaaatct
    58681 gtttatcagc aaaacctggg tcgcatctgc ggcccttttg cttttttaag ttgtaaggat
    58741 atgccatgac agaatcaacg tcccgtcgcc cggcatatgc tcgcctgttg gatcgtgcgg
    58801 tacgcattct ggcggtgcgc gatcacagtg agcaagaact gcgacgtaaa ctcgcggcac
    58861 cgattatggg caaaaatggc ccagaagaga ttgatgctac ggcagaagat tacgagcgcg
    58921 ttattgcatg gtgccatgaa catggctatc tcgatgacag ccgatttgtt gcgcgcttta
    58981 tcgccagccg tagccgcaaa ggttatggac ctgcacgtat tcgtcaggaa ctgaatcaga
    59041 aaggtatttc ccgcgaagcg acagaaaaag cgatgcgtga atgtgacatc gactggtgcg
    59101 cactggcgcg cgatcaggcg acgcgaaaat atgatgaacc tttgccaact gtcttttcag
    59161 aaaaagttaa gatccagcgt tttctgctct atcgtggcta tctgatggaa gatatccagg
    59221 atatttggcg aaattttgcc gactgaacgc atacggtatt ttacttccca gtcaagaaaa
    59281 cttatcttat tcccactttt cagttaccag cccggcggtt aagacacgct ggagctggtg
    59341 gcgatatttc gttagcttga tttcaggata attatgagca agagcaccgc tgagatccgt
    59401 caggcgtttc tcgacttttt ccatagtaag ggacatcagg tagttgccag cagctccctg
    59461 gtaccccata acgacccaac tttgttgttt accaacgccg ggatgaatca gttcaaggat
    59521 gtgttccttg ggctcgacaa gcgtaattat tcccgcgcta ccacttccca acgctgcgtg
    59581 cgtgcgggtg gtaaacacaa cgacctggaa aacgtcggtt acaccgcgcg tcaccatacc
    59641 ttcttcgaaa tgctgggcaa cttcagcttc ggcgactatt tcaaacacga tgccattcag
    59701 tttgcatggg aactgctgac cagcgaaaaa tggtttgccc tgccgaaaga gcgtctgtgg
    59761 gttaccgtct atgaaagcga cgacgaagcc tacgaaatct gggaaaaaga agtcgggatc
    59821 ccgcgcgaac gtattattcg catcggcgat aacaaaggtg cgccatacgc atctgacaac
    59881 ttctggcaga tgggtgacac tggtccgtgc ggcccgtgca ccgaaatctt ctacgatcac
    59941 ggcgaccaca tttggggtgg ccctccggga agtccggaag aagacggcga ccgctacatt
    60001 gagatctgga acatcgtctt catgcagttc aaccgccagg cagatggcac gatggaaccg
    60061 ctgccgaagc cgtctgtaga taccggtatg ggtctggagc gtattgctgc ggtgctgcaa
    60121 cacgttaact ctaactatga catcgacctg ttccgcacgt tgatccaggc ggtagcgaaa
    60181 gtcactggcg cgaccgatct gagcaataaa tcgctgcgcg taatcgctga ccacattcgt
    60241 tcttgtgcgt tcctgatcgc ggatggcgta atgccgtcca atgaaaaccg tggttatgta
    60301 ctgcgtcgta tcattcgtcg cgcagtgcgt cacggcaata tgctcggcgc gaaagaaacc
    60361 ttcttctaca aactggttgg tccgctgatc gacgttatgg gctctgcggg tgaagacctg
    60421 aaacgccagc aggcgcaggt tgagcaggtg ctgaagactg aagaagagca gtttgcccgt
    60481 actctggagc gcggtctggc gttgctggat gaagagctgg caaaactttc tggtgatacg
    60541 ctggatggtg aaacggcttt ccgtctgtac gacacctatg gcttcccggt tgacctgacg
    60601 gctgatgttt gtcgtgagcg caacatcaaa gttgacgaag ctggttttga agcagcaatg
    60661 gaagagcagc gtcgtcgcgc gcgcgaagcc agcggctttg gtgccgatta caacgcaatg
    60721 atccgtgttg atagtgcatc tgaatttaaa ggctatgacc atctggaact gaacggcaaa
    60781 gtgaccgcgc tgtttgttga tggtaaagcg gttgatgcca tcaatgcagg ccaggaagct
    60841 gtggtcgtgc tagatcaaac accattctat gcggaatccg gcggtcaggt tggtgataaa
    60901 ggcgaactga aagacgctaa cttctccttc gcggtggaag atacgcagaa atacggccag
    60961 gcgattggtc acatcggtaa acttgctgcg ggttctctga aagtgggcga cgctgtgcag
    61021 gctgatgttg atgaggctcg tcgcgcccgt attcgtctga atcactccgc aacgcacctg
    61081 atgcacgctg cgctgcgcca ggttctgggg actcatgtat cgcagaaagg ttcactggtt
    61141 aacgacaagg tgctgcgctt cgacttctca cacaacgaag cgatgaaacc ggaagagatt
    61201 cgtgcggtcg aagacctggt gaacgcacag attcgtcgca atttgccgat cgaaaccaac
    61261 atcatggatc tcgaagcggc gaaagcgaaa ggtgcgatgg cgctgttcgg cgagaagtat
    61321 gatgagcgtg tacgcgtgct gagcatgggc gatttctcca ccgagctgtg tggcggtact
    61381 cacgccagcc gcactggtga tattggtctg ttccgcatca tctctgaatc gggtactgct
    61441 gcaggcgttc gtcgtatcga agcggtaacc ggagaaggcg ctatcaccac cgttcatgca
    61501 gacagcgatc gcttaagcga agtcgcgcat ctgctgaaag gcgatagcaa taatctggcg
    61561 gataaagtgc gctcagtact ggaacgtacg cgtcagttag aaaaagaact acaacagctt
    61621 aaagaacaag ctgccgcaca ggagagcgca aatctttcca gtaaggcaat tgatgttaat
    61681 ggtgttaagc tgttggttag cgagcttagc ggtgttgagc cgaaaatgtt gcgtaccatg
    61741 gttgacgatt taaaaaatca gctggggtcg acaattatcg tgctggcaac ggtagccgaa
    61801 ggtaaggttt ctctgattgc aggcgtatct aaggacgtca cagatcgtgt gaaagcaggg
    61861 gaactgattg gtatggtcgc tcagcaggtg ggcggcaagg gtggtggacg tcctgacatg
    61921 gcgcaagccg gtggtacgga tgctgcggcc ttacctgcag cgttagccag tgtgaaaggc
    61981 tgggtcagcg cgaaattgca ataatataag cgtcaggcaa tgccgtggac tcgcttcacg
    62041 gcattcgcat taacgctatc gacaacgata aagtcaggtt gaagttgtgt atatcggcta
    62101 aacttaggtt taacagaatg taatgccatg actgcttaca tgtaatgtgt ttgtcattgc
    62161 ttactttttg gcgttatatg atggataatg ccgggataca gagagacccg actcttttaa
    62221 tctttcaagg agcaaagaat gctgattctg actcgtcgag ttggtgagac cctcatgatt
    62281 ggggatgagg tcaccgtgac agttttaggg gtgaagggca accaggtacg tattggcgta
    62341 aatgccccga aggaagtttc tgttcaccgt gaagagatct accagcgtat ccaggctgaa
    62401 aaatcccagc agtccagtta ctaatctttc cgcgtctcat ctttatcggt gagacgcacc
    62461 ctcaaaattt ctccttcact ctatagtctt ttcgctttac tcccgttcat tcaacttaag
    62521 tctccatttt tttgcattac tgctatctgc cagacctccg tttttctgta gataaaacac
    62581 tctttttgac gtttttacag actaattgag cgtgaagtgt gcaaacgata aaagtgtggg
    62641 aaaaattgtt tgacttataa gtctcagaaa gtaatatgtg cgccacgcag cgacgatgag
    62701 cgataaacaa gttcttcgaa gcactcgtaa gaggcgtgtg gtgaggtggc cgagaggctg
    62761 aaggcgctcc cctgctaagg gagtatgcgg tcaaaagctg catccggggt tcgaatcccc
    62821 gcctcaccgc cat
//
LOCUS       DADLFA010000004        59501 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000004 DADLFA010000000
VERSION     DADLFA010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59501
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.004"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(128..607)
                     /gene="folK"
                     /locus_tag="JQH65_000203"
     CDS             complement(128..607)
                     /gene="folK"
                     /locus_tag="JQH65_000203"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HAY6137726.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQDRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDELSKW"
     gene            complement(604..2022)
                     /gene="pcnB"
                     /locus_tag="JQH65_000204"
     CDS             complement(604..2022)
                     /gene="pcnB"
                     /locus_tag="JQH65_000204"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HAY6137727.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTA"
     gene            complement(2061..2987)
                     /gene="gluQRS"
                     /locus_tag="JQH65_000205"
     CDS             complement(2061..2987)
                     /gene="gluQRS"
                     /locus_tag="JQH65_000205"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414686.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HAY6137728.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIVGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQVEAHWQDFS
                     VEQILQSAVKNWTLTAVPESAIVNSTFSNASC"
     gene            complement(3024..3479)
                     /locus_tag="JQH65_000206"
     CDS             complement(3024..3479)
                     /locus_tag="JQH65_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding transcription factor
                     DksA"
                     /protein_id="HAY6137729.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3657..4361)
                     /gene="sfsA"
                     /locus_tag="JQH65_000207"
     CDS             complement(3657..4361)
                     /gene="sfsA"
                     /locus_tag="JQH65_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAY6137730.1"
                     /translation="MEFSPLLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATRGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMNVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4376..4906)
                     /gene="thpR"
                     /locus_tag="JQH65_000208"
     CDS             complement(4376..4906)
                     /gene="thpR"
                     /locus_tag="JQH65_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HAY6137731.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALMQ"
     gene            4980..7409
                     /gene="hrpB"
                     /locus_tag="JQH65_000209"
     CDS             4980..7409
                     /gene="hrpB"
                     /locus_tag="JQH65_000209"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HAY6137732.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLEELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDDAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGLLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQSPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7605..10127
                     /gene="mrcB"
                     /locus_tag="JQH65_000210"
     CDS             7605..10127
                     /gene="mrcB"
                     /locus_tag="JQH65_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HAY6137733.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRAHLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGLVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTDTWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWAGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10347..12536
                     /gene="fhuA"
                     /locus_tag="JQH65_000211"
     CDS             10347..12536
                     /gene="fhuA"
                     /locus_tag="JQH65_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HAY6137734.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMHQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIAGDGHTLNRGTVVDNERLQNFSVDTQLESKFATGDVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGSAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRISYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSAAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            12587..13384
                     /gene="fhuC"
                     /locus_tag="JQH65_000212"
     CDS             12587..13384
                     /gene="fhuC"
                     /locus_tag="JQH65_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HAY6137735.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSSKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGDTLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13384..14274
                     /gene="fhuD"
                     /locus_tag="JQH65_000213"
     CDS             13384..14274
                     /gene="fhuD"
                     /locus_tag="JQH65_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HAY6137736.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSAEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14271..16253
                     /gene="fhuB"
                     /locus_tag="JQH65_000214"
     CDS             14271..16253
                     /gene="fhuB"
                     /locus_tag="JQH65_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HAY6137737.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWMWASGALLDDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLYRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATSTMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16288..17568)
                     /gene="hemL"
                     /locus_tag="JQH65_000215"
     CDS             complement(16288..17568)
                     /gene="hemL"
                     /locus_tag="JQH65_000215"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HAY6137738.1"
                     /translation="MSKSENLYSAARELLPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPQPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGTPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17793..19214
                     /gene="clcA"
                     /locus_tag="JQH65_000216"
     CDS             17793..19214
                     /gene="clcA"
                     /locus_tag="JQH65_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HAY6137739.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMEALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVVVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSEILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19238..19303
                     /locus_tag="JQH65_000217"
     CDS             19238..19303
                     /locus_tag="JQH65_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6137740.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19296..19640
                     /gene="erpA"
                     /locus_tag="JQH65_000218"
     CDS             19296..19640
                     /gene="erpA"
                     /locus_tag="JQH65_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HAY6137741.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19687..20310)
                     /locus_tag="JQH65_000219"
     CDS             complement(19687..20310)
                     /locus_tag="JQH65_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HAY6137742.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20353..21147)
                     /gene="btuF"
                     /locus_tag="JQH65_000220"
     CDS             complement(20353..21147)
                     /gene="btuF"
                     /locus_tag="JQH65_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005024031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HAY6137743.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASPGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFDVQAHVLSSLHNSSVMRFHR"
     gene            complement(21140..21838)
                     /gene="mtnN"
                     /locus_tag="JQH65_000221"
     CDS             complement(21140..21838)
                     /gene="mtnN"
                     /locus_tag="JQH65_000221"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAY6137744.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHGKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21922..23439
                     /gene="dgt"
                     /locus_tag="JQH65_000222"
     CDS             21922..23439
                     /gene="dgt"
                     /locus_tag="JQH65_000222"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HAY6137745.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRMKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23569..24993
                     /gene="degP"
                     /locus_tag="JQH65_000223"
     CDS             23569..24993
                     /gene="degP"
                     /locus_tag="JQH65_000223"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HAY6137746.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25148..26305
                     /locus_tag="JQH65_000224"
     CDS             25148..26305
                     /locus_tag="JQH65_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family transcriptional regulator"
                     /protein_id="HAY6137747.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGRLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIYRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(26394..26780)
                     /locus_tag="JQH65_000225"
     CDS             complement(26394..26780)
                     /locus_tag="JQH65_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HAY6137748.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(26942..27754)
                     /gene="yaeI"
                     /locus_tag="JQH65_000226"
     CDS             complement(26942..27754)
                     /gene="yaeI"
                     /locus_tag="JQH65_000226"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HAY6137749.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWEPGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSVGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLMLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(27809..28633)
                     /gene="dapD"
                     /locus_tag="JQH65_000227"
     CDS             complement(27809..28633)
                     /gene="dapD"
                     /locus_tag="JQH65_000227"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HAY6137750.1"
                     /translation="MQQLQNIIETAFERRAEITPTNADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGHVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28664..31336)
                     /gene="glnD"
                     /locus_tag="JQH65_000228"
     CDS             complement(28664..31336)
                     /gene="glnD"
                     /locus_tag="JQH65_000228"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HAY6137751.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(31398..32192)
                     /gene="map"
                     /locus_tag="JQH65_000229"
     CDS             complement(31398..32192)
                     /gene="map"
                     /locus_tag="JQH65_000229"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAY6137752.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            32560..33285
                     /gene="rpsB"
                     /locus_tag="JQH65_000230"
     CDS             32560..33285
                     /gene="rpsB"
                     /locus_tag="JQH65_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAY6137753.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            33543..34394
                     /gene="tsf"
                     /locus_tag="JQH65_000231"
     CDS             33543..34394
                     /gene="tsf"
                     /locus_tag="JQH65_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAY6137754.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            34541..35266
                     /gene="pyrH"
                     /locus_tag="JQH65_000232"
     CDS             34541..35266
                     /gene="pyrH"
                     /locus_tag="JQH65_000232"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAY6137755.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            35558..36115
                     /gene="frr"
                     /locus_tag="JQH65_000233"
     CDS             35558..36115
                     /gene="frr"
                     /locus_tag="JQH65_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAY6137756.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            36207..37403
                     /gene="ispC"
                     /locus_tag="JQH65_000234"
     CDS             36207..37403
                     /gene="ispC"
                     /locus_tag="JQH65_000234"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HAY6137757.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQVACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            37591..38349
                     /gene="ispU"
                     /locus_tag="JQH65_000235"
                     /gene_synonym="uppS"
     CDS             37591..38349
                     /gene="ispU"
                     /locus_tag="JQH65_000235"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HAY6137758.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQECIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFIDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            38362..39219
                     /gene="cdsA"
                     /locus_tag="JQH65_000236"
     CDS             38362..39219
                     /gene="cdsA"
                     /locus_tag="JQH65_000236"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAY6137759.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVSLLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            39231..40583
                     /gene="rseP"
                     /locus_tag="JQH65_000237"
     CDS             39231..40583
                     /gene="rseP"
                     /locus_tag="JQH65_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HAY6137760.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GLQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSLLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGAHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            40613..43045
                     /gene="bamA"
                     /locus_tag="JQH65_000238"
     CDS             40613..43045
                     /gene="bamA"
                     /locus_tag="JQH65_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HAY6137761.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            43167..43652
                     /gene="skp"
                     /locus_tag="JQH65_000239"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             43167..43652
                     /gene="skp"
                     /locus_tag="JQH65_000239"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HAY6137762.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43656..44681
                     /gene="lpxD"
                     /locus_tag="JQH65_000240"
     CDS             43656..44681
                     /gene="lpxD"
                     /locus_tag="JQH65_000240"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HAY6137763.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44786..45241
                     /gene="fabZ"
                     /locus_tag="JQH65_000241"
     CDS             44786..45241
                     /gene="fabZ"
                     /locus_tag="JQH65_000241"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAY6137764.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            45245..46033
                     /gene="lpxA"
                     /locus_tag="JQH65_000242"
     CDS             45245..46033
                     /gene="lpxA"
                     /locus_tag="JQH65_000242"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HAY6137765.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVGIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFSRSTRGLIR"
     gene            46033..47181
                     /gene="lpxB"
                     /locus_tag="JQH65_000243"
     CDS             46033..47181
                     /gene="lpxB"
                     /locus_tag="JQH65_000243"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HAY6137766.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDFSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKISHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            47178..47774
                     /gene="rnhB"
                     /locus_tag="JQH65_000244"
     CDS             47178..47774
                     /gene="rnhB"
                     /locus_tag="JQH65_000244"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAY6137767.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMVVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            47811..51293
                     /gene="dnaE"
                     /locus_tag="JQH65_000245"
     CDS             47811..51293
                     /gene="dnaE"
                     /locus_tag="JQH65_000245"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAY6137768.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGVNAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYASGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            51306..52265
                     /gene="accA"
                     /locus_tag="JQH65_000246"
     CDS             51306..52265
                     /gene="accA"
                     /locus_tag="JQH65_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAY6137769.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSSYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            52364..54473
                     /gene="ldcC"
                     /locus_tag="JQH65_000247"
                     /pseudo
     CDS             52364..54473
                     /gene="ldcC"
                     /locus_tag="JQH65_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
     gene            54530..54919
                     /locus_tag="JQH65_000248"
     CDS             54530..54919
                     /locus_tag="JQH65_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAY6137770.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54984..56282
                     /gene="tilS"
                     /locus_tag="JQH65_000249"
     CDS             54984..56282
                     /gene="tilS"
                     /locus_tag="JQH65_000249"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAY6137771.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELAQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGKSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKSAGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(56331..56591)
                     /gene="rof"
                     /locus_tag="JQH65_000250"
     CDS             complement(56331..56591)
                     /gene="rof"
                     /locus_tag="JQH65_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HAY6137772.1"
                     /translation="MLMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(56578..56778)
                     /locus_tag="JQH65_000251"
     CDS             complement(56578..56778)
                     /locus_tag="JQH65_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HAY6137773.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56944..57489
                     /locus_tag="JQH65_000252"
     CDS             56944..57489
                     /locus_tag="JQH65_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HAY6137774.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            57486..57908
                     /gene="arfB"
                     /locus_tag="JQH65_000253"
     CDS             57486..57908
                     /gene="arfB"
                     /locus_tag="JQH65_000253"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAY6137775.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSAKAMRGKVRSGRE"
     gene            57922..58632
                     /gene="nlpE"
                     /locus_tag="JQH65_000254"
                     /gene_synonym="cutF"
     CDS             57922..58632
                     /gene="nlpE"
                     /locus_tag="JQH65_000254"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308221.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HAY6137776.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58832..>59501)
                     /locus_tag="JQH65_000255"
     CDS             complement(58832..>59501)
                     /locus_tag="JQH65_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HAY6137777.1"
                     /translation="TEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAI
                     FLGDNNVAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGY
                     NYRGIVEFIPFMVTRQMCSLNQFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVT
                     DAFAKAGHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFV
                     GLTR"
BASE COUNT        14075 a        14663 c        16459 g        14304 t
ORIGIN      
        1 tgggattgat atttatctgc gggaatttat attatgtttc tgcaaaaatg tttaaactaa
       61 aatttttatt aaggaaagag aggatgtatg attttcagaa aatatttata ttcgtaatat
      121 aaataaatta ccatttgctt aattcgtcaa atgctcttgt atgtaagact tcacgcaacg
      181 tctccccatc aggaaacgcc agctccggtg caatctcaaa cagcggccac agcataaatc
      241 cacgattctt catatcgtag tgcggaacgg tcaggcgttc ggtatttatc acttcattac
      301 caaacagcat gatatcgaga tccagcgtgc gtggtcccca gcgttcagct ttacgaacac
      361 gatcctgttg cagttctatg cgctgtgtat ggttgagtaa ctcttccggg gcgagcgaag
      421 tttcaagcgc cacggcggcg ttgaggtaat cgggttgatc ttgcggcccc agcggtggag
      481 tgcggtaaaa cgaagaaacg gcaagaatgt ggctttcagg gatatcgcct aatgctttca
      541 gggcagcatt gacctgctcc agcggagagg ccagattgct gcctatggca atatacgcca
      601 ctgtcatgcg gtaccctcac gacgcggtgc gcgtttgcgt ggacgacgag tacggcgacg
      661 cggtgacggc tcttcatcca actcgttaag catccctttt tgatctggtg gcgcggaaac
      721 ctggaactca ccccaccatt tcaccagacg ctgcagttca gcgttacgtt caacttcagc
      781 tcgcaaggcc aacaggtcat aagccgcacg gaacttagga tgctccagca gtttccatgc
      841 gcgtttaccc tgacgacggg acatacgcaa ctgcaactgc cagatatcgc gggttaacgt
      901 cgtcagacgt ttcgggattg ccagtgaacg gcaggcttcg tccagcacgt cgttcatcgc
      961 cagcgcgaaa gcgtcgtgat aggtcaggcc gctttcctgg gcgatcttct gtgccgtctc
     1021 cagcagtggg taccagaaca tggcggcaaa caagaacgcc gggttcacgc gcatatcgtt
     1081 atggatacgc gtatcggtat tcttcagcac ctgttcaatg atccgctcca tcgggctgtc
     1141 gccattttcc gtgaagtagc gggtaatggt cgggaacagc ggctggaaca gatgatattc
     1201 acacaacagc ttataggttt cgtaaccgta gcccgcttgt agcagtttaa gcgattcttc
     1261 aaacaggcgt gccggtggga tatcgttcag cagggtagcg aggcgaggga tcggttctgc
     1321 ggtttccggg ctgatgcgca tacccaattt ggcggcaaaa cgtaccgcgc gcagcatacg
     1381 taccggatct tcacggtagc gcgtttccgg gttaccaatc agacggataa cgccgtcctt
     1441 cagatccttc atgccgccaa cgtaatcacg gacggtaaaa tccgctacgc tgtaatacag
     1501 gctgttgata gtgaaatcgc ggcgctgggc gtcttcttcg atggagccga aaatgttgtc
     1561 gcgcagcaac atgccgtttt gcccgcgttg ggaggtcgtg cggtcgctga cgttaccttc
     1621 gtggtgtcca cggaaggttg caacttcgat aatctccggg ccaaacatca catgagccag
     1681 acggaaacgg cgacccacaa ggcggcagtt acggaacagt ttgcgcacct gctcaggcgt
     1741 ggcgttagtg gttacgtcaa aatctttcgg ctttttgcca agtaacaggt cgcgcacgcc
     1801 gccgccaacc agccaggctt cgtatcccgc tttattgagc ctgtacatta ccttcagggc
     1861 attttcactg atatctttgc gggaaatagc atgctgctca cgcgggatca ccgtcacctg
     1921 tggacgggcg actgcctgtt cagcctcgct ttcctcgcgg cttagcacct tgcggcaaaa
     1981 attagcgact cgggtaaaaa tagtacacct cggtagtgtc aaacatcatt caggacaaaa
     2041 aaatagcggc taatcatagc tcagcatgac gcatttgaga atgttgaatt tacaattgcc
     2101 gactcgggca cggcggtcag cgtccagttt ttgacggctg actgaaggat ttgctcgacg
     2161 ctgaaatcct gccagtgtgc ttctacctgc tgccccagaa attgaagtgc cgcgattagt
     2221 accgggcgtg gatcgccttt cggcaacgca ggcgcatgat tctgcttgga aagtttagcg
     2281 ccttgtggat taagcgccag cggcagatga atgtaatctg gcactttcca gccaaaaagc
     2341 tggtacagcg agatttgcct tactgttggt tcaatcagat cagccccacg cactatttct
     2401 gtaacgccct ggaaatgatc atcaaccaca acagccaggt tgtaggcgaa caacccatca
     2461 cggcgatgaa tgataaaatc ttcccgtgcc agtttttcgt cggcgtgaat aatgccgcgc
     2521 agctggtcag taaattgcgt gaccggatgc tgctggcgga tacgcactgc ggcgttgtct
     2581 ggtccatgat gcaacacccg gcaatgaccg tcgtaaatac cgacaatgct ttgaatacgc
     2641 gcacgcgtac aggtgcagta ataacttagt ccttgttcat gtaaccaggc gagtgcttca
     2701 cgataggcgt cgtgacgttg cgattgccag agaacatcgc cgtcccagtg cagaccgtaa
     2761 tgttccagct ggcgcaggat agtttctgcg gcaccgggaa cttcacgagg cgggtcgata
     2821 tcttctatgc gtaccagcca gcgaccttgc cgggcgcgag cctgcaaata gctgccgagc
     2881 gcggcgatca gagagccaaa atgaagctcg ccggaaggag agggggcgaa gcggccaata
     2941 tactgtgtgt ctgtcatctc tttgaacaaa aaataaggcg ggagcatttc ccgcctgtgg
     3001 taaacgtgat ggaacggctg taattagcca gccatctgtt tttcgcgaat ttcagccagc
     3061 gttttgcagt cgatgcacag atcggctgtc gggcgcgctt ccagacggcg aataccaatt
     3121 tcaacaccgc aggattcgca gtagccgaaa tcttcgtctt ccactttttt cagcgtcttc
     3181 tcgatctttt tgatcagctt acgctcgcga tcgcggttac gcagttcgag gctgaactct
     3241 tcttcctggg ctgcacggtc taccgggtcc gggaagttgg ctgcttcatc ctgcatatgt
     3301 gtaacggtgc gatcgacttc atccctgagt tgattacgcc atgcttccag aatacgacgg
     3361 aagtgcgcca gctgggcttc attcatatac tcttcgcccg gcttctcctg atatggttcc
     3421 accccagcga tggcgagaat actcagggac gatgttttac ggttttgccc ttcttgcatg
     3481 ttgcttctcc ttaacacgca ctatcgatcc ccatgttcgg gggaaaaatg aggccgctat
     3541 aaatagcaga tgcttttccg gatagcaatt atctaaacgt aacacttgac aactgtgtga
     3601 ggaaaagcgt atttgcgcac gcgaccagaa tgtaaattaa ccagttactt actttactac
     3661 aatgtaaccg gcagtgattt tttaagagcc atgccttcag cagaaatttc cgctttgtaa
     3721 gccagaattt ctacccccct ctgttgagct tctgacaata gttgcgcgta tttctcatcg
     3781 atgtggcgcg cgggtgaaaa ccgtgtaatg gctgaatgca gcacggcgaa aaagataacc
     3841 gcacgctggc cttcagccgc tacgttcatc aactcccgaa ggtgtttctg acctcgttca
     3901 gtgaccgcat cgggaaaata tccctgttcg ttctccgcta acgtaaccga tttcacttca
     3961 atatagcagt ctggacgcga atccgcctgc aacataaagt caatacggct gcgttctgcg
     4021 ccatatttta cttcgctttt cagcgagcta tagcctgaca gttctgaaat tgattcatta
     4081 aggatagcct ctttcgtcaa cctgttagcc caaagcgtat tgacgcaaat aaatgcgccg
     4141 ctctggcttt gagttagttc ccaggtgtgt gggtatttcc gtttggtgtt gtctgaagtc
     4201 gaataccaga cggtatcgcc acgcgttgca caaccggtca tcgcacccgt attcgggcag
     4261 tgtagcgtta attcgcgacc atcgggtgtg atcacatcgg ctaaaaaacg tttgtaacgc
     4321 tgaattagcg tcgcgcgctg tagaagggga gagaattcca ttgcgacaat ccttgttatt
     4381 gcattagcgc ccagcgtttt agcggcgtgt agcgtgtgcg tccacgggca aacgaggagg
     4441 cgtaaagggt gaactccgtc accgcataag accagttaaa acctggcggc gggattgtca
     4501 ccgcctcgct ggcgtcgcgc aataaggtaa tatgtggatg aaacggacga ttgctttgaa
     4561 aacaaccgct gcgggcagcc tgtgaacgga gcatattcgc cagctggatt aagccgcgtg
     4621 gcggctgacg catccctaac cacaccacac gcgaacgcag ccattgtccg gcgtcatcaa
     4681 gcgtgagtgt gaaaccaggt tgacgaatcc gtccggctaa aagagaaagc gccttctctt
     4741 tctcggcgct cacttcgcct aaaaatgcca gagtcagatg caaattatcg gcggcgaccg
     4801 gacgtcccgc ctcaggtggg aagtgtgtgg cgcgccagtg gataatctgt tcgcggattt
     4861 ctgcaggtaa gtcgatagca aagaacagac gttgcggttc agacatgtga ggcactcggt
     4921 tatgaattac cgcgatgcta caatgtggcg cgaagaatgt taaccctctg gagcgttttg
     4981 tgtcgtcgtt gcccgttgct gccgttttac ctgaattact taccgctctc gattgtgcgc
     5041 cgcaggtatt attaagtgcg ccgaccgggg ccgggaaatc aacctggctg ccgctgcaac
     5101 tgctggcgca tcccggcatt aacgggaaaa ttatcctgct ggagccgcgt cgtctggcgg
     5161 cgcgtaacgt cgcgcaacgg ctggaggagc tgcttaacga aaagccaggc gataccgttg
     5221 gctaccggat gcgtgcgcaa aactgcgtcg ggccgaatac ccgcctggaa gtagttaccg
     5281 aaggcgtgct gacgcggatg atccagcgtg acccggagtt gagcggtgtt ggattggtga
     5341 tccttgatga atttcatgag cgtagcttgc aggcggattt ggcgttggcg ctgttactcg
     5401 atgtgcaaca aggtctgcgt gatgacctta aactgctaat tatgtcggct acgctggaca
     5461 acgaccgctt gcagcaaatg ctgccagaag cgcccgtcgt catctcagaa gggcgctcgt
     5521 ttccggttga acgccgttat ttaccgctgc ccgcgcatca gcgttttgac gatgccgttg
     5581 cggtagccac cgctgaaatg ctgcgtcagg aaagcggatc attactgtta tttttacctg
     5641 gcgtcggaga aattcagcgt gtgcaggaac aactggcttc gcgcatcggc agtgatgtat
     5701 tgctctgccc gctgtatggc gcgttgtcgc tgaacgatca gcgaaaagcg atcctcccgg
     5761 caccgcaagg gatgcgcaaa gtggtgctgg cgaccaatat tgctgaaacc agtttaacca
     5821 tcgaaggtat tcgtctggtg gtggattgtg cccaggagcg tgtggcgcgt tttgatccgc
     5881 gcacggggct aacgcgactg attactcaac gcgttagcca ggcatccatg acgcagcgtg
     5941 ccgggcgcgc cgggcgtctg gagccgggta tctgcctgca tctaatcgcc aaagaacaag
     6001 cagaacgcgc tgccgcgcaa agtgaaccgg aaatcttaca aagcgatctt tccggtttgc
     6061 tgatggaatt actgcaatgg ggatgcagcg atccggcgca gatgagctgg ctggatcaac
     6121 cgccaacggt gaatctactg gccgcgaaac gcctgttaca aatgctgggg gcgctggagg
     6181 gtgaacggct tagtgcgcag gggcaaaaaa tggcagcgct gggtaacgat ccgcgtttag
     6241 cggcaatgct ggttagcgcg aagaacgacg acgaagctgc taccgcggca aaaattgccg
     6301 ccattctcga agagccgcca cggatgggca atagtgacct gggcgtggcg ttttcgcgca
     6361 atcaaccagc ctggcagcaa cgtagtcagc aactgttaaa acgcttaaac gtacgtggcg
     6421 gtgaggcaga cagttcgctt atcgcgccgc tacttgccgg ggcgtttgcc gatcgcattg
     6481 ctcgtcgccg tgggcaagat ggacgctatc aactggcaaa cggcatggga gcgatgctcg
     6541 atgccgacga cgcgctaagc cgccacgaat ggttgatcgc accgttatta ttgcagggca
     6601 gcgcctcgcc ggatgcgcgg attttactgg cgctgccggt cgatattgat gagttagtac
     6661 aacgctgccc gcagctggta cagcagtctg acactgtgga gtgggatgac gcgcaaggta
     6721 cgctgaaagc ctggcgtcgg ctgcaaatcg gtctgctgac ggtgaaagtg cagccgctgg
     6781 cgaaaccctc ggaagacgag ttgcatcagg cgatgcttaa tggcatccgt gataaaggtt
     6841 taagcgtgct caactggacg gcggaagcgg aacagctacg cttgcgtttg ttatgcgccg
     6901 caaagtggtt gccggaatat gactggccag cggttgatga tgaaagttta ttggcgacgc
     6961 tggaaacgtg gctgctgcca catatgactg gcgtacattc actacgcggc ctgaaatcac
     7021 tcgacattta tcaggcacta cgcggattac ttgattgggg aatgcagcaa cgtctggata
     7081 gtgaattgcc tgcgcattac actgtgccga cgggaagccg gatcgccatt cgttatcatg
     7141 aagataaccc gcccgcgctg gcggtgagaa tgcaagagat gtttggcgag gccactaatc
     7201 cgacgatcgc ccaggggcgc gtgccgctgg tgctggagtt gctttcacct gcccaaagtc
     7261 cattacaaat cacacgagat ttgagcgcct tctggaaagg agcgtaccgt gaggtgcaaa
     7321 aagagatgaa agggcgttat cccaaacatg tctggccgga cgacccggca aatactgcac
     7381 cgacgcgacg gacgaaaaag tattcgtaag gttggttttc tccctctccc tgtgggagag
     7441 ggccggggtg agggcatcag cgcgcacgtt caccctcacc ctaaccctct ccctcaaggg
     7501 agaggggacc gatcgaacac aaattttgag agatatcttc ttctgtcttg taacagaaga
     7561 acagaaaatc gggcttttgc gcctgaatat tgcggagaaa aagcatggcc gggaatgacc
     7621 gcgagccaat tggacgcaaa gggaaaccga cgcgtccggt caaacaaaag gtaagccgtc
     7681 gtcgttacga agatgacgat gattacgacg attatgatga ctatgaggat gaagaaccga
     7741 tgccgcgcaa aggtaagggc aaaggcaaag ggcgtaagcc tcgtggcaaa cgcggctggc
     7801 tatggctact gctaaaactg gctatcgttt ttgccgtgct gatcgccatt tacggcgttt
     7861 atctcgatca aaaaattcgt agccgtattg atggcaaggt ctggcaactg cctgcagcag
     7921 tttatggccg aatggtcaat cttgagccag atatgaccat cagcaagaac gagatggtga
     7981 agctgctgga ggcgactcag tatcgtcagg tgtcgaaaat gacccgtcct ggcgaattta
     8041 ccgtgcaggc caacagcatt gagatgatcc gccgtccgtt tgatttcccg gacagtaaag
     8101 aagggcaggt gcgtgcgcat ctgacctttg atggtgatca tctggcgacc atcgtcaata
     8161 tggagaacaa ccgtcagttc ggctttttcc gtcttgatcc gcgtctgatc accatgatct
     8221 cctcgccaaa cggtgagcaa cgtctgtttg tgccgcgcag tggtttccct gatttgctgg
     8281 tggatacttt gctggcgaca gaagaccgcc atttctacga gcatgatggg atcagcctct
     8341 actcaattgg gcgtgcggtg ctggcaaacc tgaccgcagg tcgcacggtg cagggtgcga
     8401 gtacgctgac gcaacagctg gtgaaaaacc tgttcctctc cagcgagcgc tcttactggc
     8461 gtaaagcgaa cgaagcctac atggcgctga tcatggacgc gcgttacagc aaagaccgta
     8521 ttcttgagct gtatatgaac gaggtgtatc tcggtcagag cggcgacaac gaaatccgcg
     8581 gcttcccgct ggcaagcttg tattactttg gtcgcccggt agaagagcta agcctcgacc
     8641 agcaggcgct gttagtcggt atggtgaaag gggcgtccat ctacaacccg tggcgtaacc
     8701 cgaaactggc gctggagcga cgtaatctgg tgctgcgtct gctgcaacag caacagatta
     8761 ttgatcaaga actctatgac atgttgagtg cccgtccgct gggggttcag ccgcgcggtg
     8821 gggtgatctc tcctcagcca gcctttatgc aactggtgcg tcaggagctg caggcaaaac
     8881 tgggcgataa ggtaaaagat ctctccggcg tgaagatctt cactaccttt gactcggtgg
     8941 cccaggacgc ggcagaaaaa gccgccgtgg aaggcattcc ggcactgaag aaacagcgta
     9001 agttgagcga tcttgaaact gcgattgtgg tcgtcgaccg ctttagtggt gaagttcgtg
     9061 cgatggtggg aggttctgag ccgcagtttg cgggctacaa ccgtgcgatg caggcgcgtc
     9121 gttcgattgg ttcccttgca aaaccagcga cttatctgac ggctttaagc cagccgaaaa
     9181 tctatcgcct gaacacgtgg attgccgatg cgccaatcgc gctgcgtcag ccgaatggcc
     9241 tggtctggtc accgcagaac gatgaccgtc gttatagcga aagcggcaga gtgatgctgg
     9301 tggatgcgtt gacccgttcg atgaacgtgc cgacggtaaa tctggggatg gcgctggggc
     9361 tgcctgcagt tacggatacc tggatcaaac tgggcgtacc gaaagatcag ctgcatccgg
     9421 ttccggcgat gctgctgggg gcgttgaact taacgccaat cgaagtggcg caggcgttcc
     9481 agaccatcgc cagcggtggt aaccgtgcac cgctttctgc actgcgttcg gtaatcgcgg
     9541 aagatggcaa ggtgctgtat cagagcttcc cgcaggcaga acgcgctgtt ccggcgcagg
     9601 cggcgtatct gacactatgg accatgcagc aggtggtaca acgtggtacg ggtcgtcagc
     9661 ttggggcgaa atacccgaac ctgcatctgg cagggaaaac agggactacc aacaataacg
     9721 tagatacttg gtttgcaggc attgacggca gcacggtgac catcacctgg gcaggccgtg
     9781 ataacaacca gccgaccaaa ctgtatggag ccagcggggc aatgtcgatt tatcagcgtt
     9841 atctggctaa ccagacgcca acgccgctga atcttgttcc gccagaagat attgcagata
     9901 tgggcgtgga ctacgacggc aactttgttt gcagcggtgg catgcgtgtc ttgccggtct
     9961 ggaccagcga tccgcaatcg ctgtgccagc agagcgagat gcagcagcag ccgtcaggca
    10021 atccgtttga tcagtcttct cagccgcaac agcaacctgc tcagcaagag cagaaagaca
    10081 gcgacggtgt agccggttgg atcaaggata tgtttggtag taattaacat ctaagcgtga
    10141 aataccggat ggcgagttgc catccggtaa aataacatcc catctaagat attaaccctt
    10201 tcttttcatc tggttgttta ttaacccttc aggaacgctc agattgcgta ccgcttgcga
    10261 acccgccagc gtttcgaata ttatcttatc tttataataa tcattctcgt ttacgttatc
    10321 attcacttta catcagagat ataccaatgg cgcgttccaa aactgctcag ccaaaacact
    10381 cactgcgtaa aatcgcagtt gtagtagcca cagcggttag cggcatgtct gtttatgcac
    10441 aggcagcggt tgaaccgaaa gaagacacta tcaccgttac cgctgcacct gcgccgcaag
    10501 aaagcgcatg ggggcctgct gcaactattg cggcgaagca ctctgctact gcgactaaaa
    10561 cggatacacc aattgaaaaa acgccgcagt ctatttcggt tgtgacaaat gaagagatgc
    10621 agatgcatca atttcagtct gtaaaagaag cattaggtta tacacctggc gttacggtta
    10681 gcagtcgtgg tgcttctaat acatatgatt ttgttatcat tcgtggtttc tcatctgttg
    10741 gtctgaatca aaataattac cttgatggac taaaacttca gggcaacttt tataacgatg
    10801 ctgtgattga tccttacatg ctcgagaggg ttgaactgat gcgtgggccg acgtctgttc
    10861 tctatggaaa aagtaatcct ggtgggatta tttctatggt gagtaagcgt ccgaccactg
    10921 aacccctgaa agaaattcag tttaaaatgg ggacggataa tctgtttcag accggattcg
    10981 atttcagtga tgcactggat gataacggtg agttctctta ccgtttgacg ggcctcgctc
    11041 gctcaacaaa cgaacagcag aaaaactctg aatctcagcg ttataccatt gctccatcat
    11101 tctcatggcg tccagacgac aaaaccaatt ttactttcct gtcctatttc cagaatgaac
    11161 cagaaacggg ttattacggt tggttgccga aagaggggac cgttgagcca ttgcctaatg
    11221 gtaagcgtct accgactgac ttcaatgaag gtgcatcgaa taatacatac tcccgtaacc
    11281 agaaaatggt gggatatagt tttgaacatg gttttaatga caccttcacc gtgcgtcaga
    11341 atctgcgttt cagtgaaatg aaaacctcac agaaaagtgt ttatggcaca gggattgctg
    11401 gtgatggtca taccctaaac cgcgggacag tggtggataa tgagcgtcta caaaacttta
    11461 gcgttgatac ccaacttgaa agtaagtttg ctacaggtga cgtcgagcat actttgctga
    11521 caggggtaga cttcatgcgg atgcgtaatg atatcaatgc cagctttgga tccgcaccat
    11581 ccatcgatct ttataacaaa tatcaccctg aatactttgc atttggtagc gcagagccat
    11641 accagatgaa tgaaagcaaa caaacaggta tttatgttca ggatcaggcg gaatggaata
    11701 aatgggtatt cactctgggg ggacgctacg attggtctaa gcaagcgacc actgttcgtg
    11761 aaaactctta tacgccgact gaaggttata ttgagcgcaa tgatcatcag ttcacctggc
    11821 gcggtggtgt aaattactta ttcgataatg gtatttcacc ttactttagc tatagccagt
    11881 cctttgaacc gagtgctttc gatctgtgga gcaacccacg catttcctat aagccatcga
    11941 aaggtgaaca gtatgaagct ggcgtaaaat atgttccgaa tgatatgccg gtcgttgtta
    12001 cgggcgcagt ctatcaattg acgaaaacaa ataacctgac agcagaccca acaaacccat
    12061 tagcgcaagt cccagcaggt gagattcgcg ctcgtggtgt ggaacttgaa gcaaaagcgg
    12121 cgttaaatgc caatattaac ttgacggctt cttataccta caccgatgcg gaatacacca
    12181 aagacaccaa tctcaaaggt aaaactccag aacaagtacc ggagcatatg gcatctctct
    12241 ggggggatta taccttcaat gaagggccgc tttctggttt aacattggga acaggtggtc
    12301 gttttattgg ttccagctat ggtgatccgg caaacacttt taaagtgggt agcgcagctg
    12361 taatggatgc tgttgtaaaa tatgatctgg cacgctttgg tatggcggga tccagccttg
    12421 ctgtgaacgt caacaatttg ctcgatcgtg agtatgttgc cagttgcttc cagacttatg
    12481 gctgcttctg gggcgcagaa cgtcaggtcg ttgcaaccgc aaccttccgt ttctaatttc
    12541 tcttttgggg cacggatttc cgtgcccatt tcacaagttg gctgttatgc aggaatacac
    12601 gaatcattcc gataccactt ttgcactgcg taatatctcc tttcgtgtgc ccgggcgcac
    12661 gcttttgcat ccgctgtcgt taacctttcc tgccgggaaa gtgaccggtc tgattggtca
    12721 caacggttct agtaaatcca ctctgctcaa aatgcttggc cgtcatcagc ctccgtcaga
    12781 aggggagatt cttcttgatg cccagccgct ggaaagctgg agcagcaaag cgtttgcccg
    12841 caaagtggct tatttgccgc agcagcttcc tccggcagaa gggatgaccg tgcgtgaact
    12901 ggtggcgatt ggtcgttacc cgtggcatgg cgcgctgggg cgctttgggg cggcagatcg
    12961 cgaaaaagtc gaggaagcta tctcgctggt tggcttaaaa ccgctggcgc atcggctggt
    13021 tgatagtctc tctggcggcg aacgtcagcg ggcgtggatc gccatgctgg tggcgcagga
    13081 tagccgttgt ctgttgctcg acgaaccgac ctcggcgctg gatatcgccc accaggttga
    13141 tgtgctggcg ctggtgcacc gtttaagtca ggagcgtggc ctgacggtca ttgccgtgtt
    13201 gcacgatatc aatatggcgg cacgctactg tgattatctg gtcgccctgc gcggtggtga
    13261 aatgattgct cagggaacgc ctgcggaaat tatgcgcggc gataccctcg aaatgattta
    13321 tggcatcccg atgggtattt tgccgcatcc ggcgggtgct gcacctgtga gttttgttta
    13381 ttgatgagcg gcttacctct tatttcgcgc cgtcgactgt taacggcgat ggcgctttct
    13441 ccgttgttat ggcagatgaa taccgcccac gcggcggcta ttgatcccaa tcgtattgtg
    13501 gcgctggagt ggttgccggt ggaattactg ctggcgctcg gcatcgtgcc ttacggcgta
    13561 gcggatacca tcaactatcg cctgtgggtc agcgaaccac cattgccgga ctcagtgatc
    13621 gacgtcggtt tgcgcacaga acctaacctt gaactgctga ccgaaatgaa accgtcgttt
    13681 atggtctggt cggcaggata tggcccttca gcagaaatgc tggcacgtat tgcgccgggg
    13741 cgtggattta acttcagcga cggcaaacag ccgctggcga tggcgcgtaa atcactgacg
    13801 gaaatggcag atttacttaa cctgcaaagc gcagcagaaa cgcatttagc gcaatatgaa
    13861 gactttatcc gcagcatgaa accccgcttt gtgaagcgtg gtgcacgccc gttattgctg
    13921 acgacgctta tcgatccgcg ccatatgttg gtcttcggtc caaacagctt gttccaggaa
    13981 attcttgatg agtacggcat cccaaatgcc tggcaagggg aaaccaactt ctggggcagt
    14041 accgccgtca gtatcgatcg tctggcagcg tataaagacg ttgatgtgct ctgttttgat
    14101 cacgacaaca gcaaagacat ggatgcgcta atggcaaccc cgctgtggca ggccatgccg
    14161 tttgtccgcg ccggacgctt tcagcgcgta cccgcagtct ggttttatgg tgcgacgctc
    14221 tcggcaatgc actttgtgcg cgttctggat aacgccatcg gaggtaaagc gtgagtaaac
    14281 gaattgcgct tttcccggcg ttattgctgg cgctgctggt gattgtcgct acggcgctga
    14341 cctggatgaa cttttcgcag gcgctgccgc gtagccagtg ggcgcaggcc gcctggtcgc
    14401 cggatattga cgtcatcgag cagatgattt ttcactacag cttgctgccg cgtctggcga
    14461 tttcgctgct ggtgggcgca ggcctggggc tggtgggcgt gctgtttcag caagtgctgc
    14521 gtaatccgct ggcggaaccg acgacacttg gcgttgctac aggcgcgcaa ctggggatta
    14581 ccgtcactac gctctgggcg atcccagggg cgatggcgag tcagtttgct gcgctggcgg
    14641 gcgcttgcgt tgttggctta atcgtctttg gcgtcgcgtg ggggaaacgg ctttcgccgg
    14701 taacgctgat cctcgcgggg ctggtagtga gcctttattg tggggcaatc aatcagttac
    14761 tggttatctt ccaccatgac caactgcaaa gcatgttcct gtggagcacc ggaacgctga
    14821 cgcaaaccga ctggggcggt gttgagcgtt tatggccgca gttgctgggc ggtgtgatgc
    14881 tgacgttgct gctacttcgc ccgttaaccc tgatggggct tgatgatggc gtggcgcgca
    14941 atctcgggct ggccttgtcg ctcgcgcgtc tggcggcgct gtcgctggcg attgtcatca
    15001 gtgcgctgct ggtgaacgcg gtggggatta tcggctttat cgggttgttt gcgccgttgc
    15061 tggcaaaaat gctgggggcg cggcgtctgc tgccgcgact gatgctggcg tcgttgattg
    15121 gtgcgctgat cctctggctt tccgatcaaa tcatcctctg gctgactcgc gtgtggatgg
    15181 aagtgtccac cggttcggtc actgcgttga tcggtgcgcc gctgctactg tggctgttgc
    15241 cgcgtttacg cagcattagc gcgccggata tgaaggtcaa cgatcgtgtc gcggctgaac
    15301 gccaacatgt gctggcgttt gccctcgcgg gcggcgtgct gctgttgatg gctgtggtgg
    15361 tggcgctgtc gtttggtcgt gatgcgcacg gctggatgtg ggcgagcggg gcgttgctcg
    15421 atgatttaat gccctggcgc tggccgcgaa ttatggcggc gctatttgcg ggcgtcatgc
    15481 tggcggtggc gggctgtatt attcagcgac tgaccggcaa cccgatggca agcccggaag
    15541 tgctggggat tagctccggc gcagcgtttg gcgtggtact gatgctgttt ctggtaccgg
    15601 gtaatgcttt tggctggctg ttacccgcgg gaagtatcgg tgccgcggtg acgctgttga
    15661 ttattatgat cgccgccggg cgcggtggat tttccccaca ccgcatgtta ctggcgggga
    15721 tggcgttaag taccgcattc accatgcttt taatgatgtt gcaggcgagt ggtgacccgc
    15781 gaatggcgca agtgctcacc tggatctctg gttcgaccta caacgccacc gatgcgcagg
    15841 tctggcgtac cggaattgtg atggtgattt tgctggcgat taccccgctg taccgccgct
    15901 ggctgaccat tttacctctg ggcggtgata ccgcacgagc tgtgggaatg gcgctgacgc
    15961 cgacgcgaat tgcgctgctg ctgttagcgg cttgcctgac ggcgacctcg acgatgacta
    16021 ttggaccgtt gagttttgtt ggtttaatgg caccgcatat tgcgcggatg atgggttttc
    16081 gacggacgat gccacacatc gtaatttcgg cgctggtggg tggtttactg ctggtattcg
    16141 ctgactggtg tgggcggatg gtgctgtttc cattccagat cccggcgggg ctgctgtcga
    16201 cctttatcgg cgcgccatat tttatctatt tgttgagaaa gcagagccgt taattgagtg
    16261 cctcgcccgg tgggcgaggc aaaattatca caacttcgca aatacccgac gtgcagcatc
    16321 gatggtgtta ttgatatctt ccatgctgtg cgccacagac ataaagcccg cttcaaacgc
    16381 tgacggtgcc aggtaaacac cttcgtccag catcatatgg aagaaacgct taaagcgttc
    16441 cacgtcacag gccatcacat cctgatagca cgtcacggac tcggcgtcgg taaagaaaat
    16501 accgaacatg ccgccaacgt ggttaacgac cagcggagtt ccggcttctt ctgccgcttc
    16561 cagcagacct tctgccagac gtgttgtcag ctcatccagc gtttcgtgaa cgcccggctg
    16621 cgcgacttca ttcagacagg cgaaacccgc tgccatcgca atcgggttac cggaaagcgt
    16681 acccgcctga tagaccggac ccgtcggggc cagcgcatcc attacatcac gacgaccacc
    16741 gaatgcgcct accggcattc caccgccgat gattttgccg aggcaggtta aatctggcac
    16801 tacgccgtaa taatcctgtg cgccagccag tgccacgcgg aagccggtca tcacttcatc
    16861 gatgatcagc aacgcgccaa attcgtcgca cagcgcacgc agacctggca ggaactctgg
    16921 ctgcggcgga acacagttca tattgcctgc caccggctcg acgataatac aggcaatctc
    16981 ttgcgggtat tgctcaaacg cggcgcgtac agaagccaga tcgttataag tacaggttaa
    17041 ggtatgtttg gcgaaatctg ccggaacacc cggcgagttt ggctggccta acgtgagtgc
    17101 gccagaaccg gctttcacca gcaggcagtc agcgtgaccg tggtaacaac cttcaaattt
    17161 aatgattttg tcgcgaccgg taaaaccacg ggccaggcgg atggcgctca tggtcgcttc
    17221 agtgccggag ttcaccatgc gcaccatatc catggtcggg accagttcgg tcaccagttg
    17281 cgccattttc acttccattt cggtcggtgc accaaagctt aaaccacgct cggcggcttc
    17341 aatcacggca ttgcggatag ccggatggtt atggcccagc accatcggcc cccaggaacc
    17401 gacataatcg atataggctt tgccatcaac atcgtacaga taagcgccgt ccgctttttc
    17461 gataaacagt ggagtgccgc ccacgccagt aaaggcgcga acaggggagt tcacaccgcc
    17521 agggagcagc tcgcgcgctg cgctgtaaag attttcagac ttactcatgg agggttcctg
    17581 attcgtagaa aaagtgaatg gctgctattc tatgttattc ataacaagtt aaatactcgt
    17641 caaacatcag gctgcttgta ctggtgcaat cctgatttct tagagtataa aagttttgtg
    17701 catttgaaac attacgcttt gcaaaggatt ttcatggaac gtgcgagtaa aatgccgtca
    17761 tcttatttgt atgaccaata agtgatcatt ggatgaaaac tgatactccc tctttagaaa
    17821 caccgcaggc cgcgcgcctg cgacgcagac aactgattcg ccaacttctt gagcgcgata
    17881 aaaccccgtt agccattttg tttatggcgg cagtcgtcgg cacgcttgtc gggctggcag
    17941 cggttgcttt tgacaaaggt gtcgcctggt tgcagaacca acgtatggag gcgctggtac
    18001 atactgctga taattatccg cttctgttaa ccgtcgcttt tctctgttcg gcggtgctgg
    18061 cgatgttcgg ctactttttg gtgcgcaaat acgcgccgga agcaggtggt tcggggatcc
    18121 cggaaattga aggggcgctg gaagatcaac gtcccgttcg ctggtggcgt gtgttgccgg
    18181 tgaaattctt tggcgggctg gggacactcg gcggaggcat ggtgctgggg cgcgaagggc
    18241 caaccgtgca gatcggcggc aatatcggtc gcatggtact cgatattttc cgcctgaaag
    18301 gtgatgaagc tcgccatacg ctgctggcaa ccggtgctgc tgcggggctg gctgcggcct
    18361 ttaacgcgcc gctggcgggt attttgttta ttatcgaaga gatgcgtccg cagtttcgct
    18421 atacgttaat ttcgattaaa gcggtattta ttggcgtcat tatgtcgacc attatgtacc
    18481 ggatttttaa tcatgaagtt gcgttgattg acgtcggtaa actttctgat gcgccgctta
    18541 atacgctgtg gctttatctg atcctcggta ttatttttgg cattttcggc cctattttta
    18601 ataaatgggt gctggggatg caggatttgc tgcaccgtgt gcacggcggc aatattacca
    18661 aatgggtgct aatgggcggt gcgattggcg gtctgtgtgg attgctgggg tttgtggcac
    18721 cagcaacgtc gggcggcggt tttaacctga ttcctatcgc taccgcaggg aatttcagca
    18781 tgggaatgct ggtgtttatc ttcgtcgcgc gggtcattac caccttactg tgcttctctt
    18841 ccggcgcgcc gggcggtatt tttgccccga tgctggcgct gggtactgtg ctgggaaccg
    18901 ctttcggaat ggttgtcgtt gagctgtttc cgcaatatca ccttgaggcg gggacttttg
    18961 ctattgccgg aatgggggcg ttactggcgg catctattcg cgcgccgtta acggggatca
    19021 ttttggttct ggagatgacc gataactacc agctcatttt gccaatgatt attaccggtc
    19081 ttggcgcaac actattagcg caatttaccg gcgggaaacc gctatattcg gagattcttg
    19141 cgcgcacgct ggcaaaacag gaagctgagc aactggcgcg aagcaaggcc gcatcagcca
    19201 gcgagaatac ttgaacgaaa taccagggta ttagataatg gcgattatta ttgggttaga
    19261 atttgcccaa ttgccgatgt cgtttggagc aaaatatgag tgatgacgta gcactgccgc
    19321 tggagtttac cgacgcagca gccaacaaag ttaaaagcct gatcgctgac gaagataacc
    19381 cgaatctgaa attacgcgtg tatatcaccg gtggcggttg cagcggcttc cagtatggtt
    19441 tcacctttga tgatcaggtg aacgaaggcg atatgaccat cgaaaaacag ggcgttggcc
    19501 tggtggttga tccgatgagc ctgcaatatc tggtcggtgg ttccgttgac tataccgaag
    19561 gactggaagg ttctcgtttc atcgtgacca acccgaacgc gaaaagcacc tgcggttgcg
    19621 gttcttcctt tagcatctaa tctgttgttc atgtgccgga taaactatcc ggcacatctt
    19681 ctctcttcaa cgcccattct catccagcgc aaacgtcggt agcttaagat gccaacgaat
    19741 agccgccagc cgaatcaata gcgtcacgac catgcccatc atactggctg tttccagtgg
    19801 tacggaaaat gtgtaataag ccgtagcgtg gacaataccg ccgataatac aggcagttgc
    19861 gtagatttct gtacgtaaaa tcatggggat ttcgcgggcc agtacatcac gaatgatccc
    19921 gccgccaacg ccagtaatga cgcccataca aaccgcgatt aacggaccgg cttccgcatt
    19981 aaaggcttta ttcacgccaa tgccgacaaa caccgccaga ccaacggcgt ccagcaccgg
    20041 caacatccat tttggtaagc gtcttggctg gcgcaccagc acgatggtca gcatgctggt
    20101 taccattgca acgaccagat cggtgggatc tttcacccaa aataccgggc cgtgatccag
    20161 cgccatgtcg cgaattgtcc cgccgcctac agcggtaacc acgcccagta ccagaacacc
    20221 aaaagggtcc atacgcaatt ttccggctaa caaaacgccg gagatggcaa ataccgctgt
    20281 gccgactata tccagccaat agacgagcat tgttcaatcc ccactgagca cctaaaaagg
    20341 tcaggcgcta atctacctgt gaaagcgcat tacagagctg ttgtgcagcg aggataatac
    20401 gtgggcttgc acgtcaaacc agtcactcgt gagaggaata acgggaattt tgagctgttc
    20461 accccagtat tgtttgattt taggaatttg gtccggtccg cctgtaatga caatcgcctg
    20521 tggcgagcgt gctaacacct gttcgcggct aacttgcggc caaggaaccc ggctgtcttt
    20581 aaagatgttt tctccgccac aaacttcgag tacctggttt tgaatcgact cttttccact
    20641 ggtaaatggc ggattaatgc cgaattgcag aaaaacacgt tttttaggtt tatcagcata
    20701 ttgcgctttc aattgcgcgt actgatccag cagggattgc gcggcttgtt cggccttatc
    20761 cggttgcgga ctccaggggg ccagttgacg taacgcattg gcaatttgtt caatgcttgt
    20821 cgcatcgacc cacatcactt ttattcccgg cgaagccagc tggtcaacct gccgctcggc
    20881 attacctcca cgccaggcaa tcaccagatc gggtttcagc gcgacaatgc gttccagatt
    20941 catcccctgc caggtggaaa cctgctcaat cttttgcgct tgtggaggat agtcggaata
    21001 gctgctgacc ccaaccggcg tgatcccggc ggcaaaggca agttcagtgt tggcgggaga
    21061 aagcgtgatg acgcgcggtg ccgcgttgag ccacagtggc gcgagaaaag acagggcgac
    21121 cagcgccctg aacagtgact tagccatgtg ccagtttctg caccagtgac tcaaccatca
    21181 ggctggactg tttagcggca acagccagga actcatcgaa gctaagatga gactgttgat
    21241 cggccacgtc ggagatggcg cgtacgacaa caaacgggac gttgaaattg tggcagacat
    21301 gggcgattgc cgtcgcttcc atctctacag caatggcctg tgggaagttg tggcggattt
    21361 tcgccagacc aacagaaccg ttgatgaaag cgtcgccgct aacaatcagg ccacgtacag
    21421 cgttaagatt cagttcggca atgcaggcct cagcggcagc gatcagttta tcgtcagctt
    21481 taaagcctgc cggacagcct ggtaactgac cgtattcata accaaatgcc gtgacatccg
    21541 cgtcgtgata acgtgcttcg tccgagacaa cgatatcgcc cactttcaac gttggtgcca
    21601 ggccaccggc agaaccggtg ttaataatca catctggctt gccgtgttcc aacagcaaag
    21661 tggcacccag cgccgcagcg actttaccga tgcccgattt cagaagcgca acctcggttc
    21721 cattcagttg gccggtatag atttcgcaac cgccgagact gatagtttga cgattttcga
    21781 ttttgtcacg cagcagcgta acttcttctt ccattgcacc aatgatgccg attttcatag
    21841 atttactcgc gataagcccg atttgaaggc atagtttacc atgcgcttac ggggaagcgt
    21901 atttctcacg cgggagagga catggcacag attgatttcc gaaaaaaaat aaactggcat
    21961 cgtcgttacc gttcaccgca gggcgttaaa accgaacatg agatcctgcg gatcttcgag
    22021 agcgatcgcg ggcgtatcat caactctccg gcaattcgtc gtctgcaaca aaagacccag
    22081 gtttttccac tggagcgcaa tgccgccgtg cgcacgcgtc ttacccactc gatggaagtc
    22141 cagcaggtgg ggcgctacat cgccaaagaa attctaagcc gtatgaaaga gcttaaatta
    22201 ctggaagcat acggcctgga tgaactgacc ggcccctttg aaagcattgt tgagatgtca
    22261 tgcctgatgc acgatatcgg caatccgccg tttggtcatt ttggcgaagc ggcgataaat
    22321 gattggtttc gccagcgttt gcacccggaa gatgccgaaa gccagcctct gactgacgat
    22381 cgctgcagcg tggcggcact acgtttacgg gacggggaag aaccgcttaa cgagctgcgg
    22441 cgcaagattc gtcaggactt atgtcatttt gaggggaatg cacaaggcat tcgtctggtg
    22501 catacattga tgaggatgaa tctcacctgg gcacaggttg gcggtatttt aaaatatacc
    22561 cgtccggcgt ggtggcgtgg cgaaacgcct gagacacatc actatttaat gaaaaagccg
    22621 ggttattatc tttctgaaga agcctatatt gcccggttgc gtaaagaact taatttggcg
    22681 ctttacagtc gttttccatt aacgtggatt atggaagcag ccgacgacat ctcctattgt
    22741 gtggcagacc ttgaagatgc ggtagagaaa agaatattta ccgttgagca gctttatcat
    22801 catttgcacg aagcgtgggg ccagcatgag aaaggttcgc tcttttcgct ggtggttgaa
    22861 aatgcctggg aaaaatcacg ctcaaatagt ttaagccgca gtacggaaga tcagtttttt
    22921 atgtatttac gggtaaacac cctaaataaa ctggtaccct atgcggcaca acgatttatt
    22981 gataatctgc ctgcgatttt cgccggaacg tttaatcatg cattattgga agatgccagc
    23041 gaatgcagcg atcttcttaa gctatataaa aatgtcgctg taaaacatgt gtttagccat
    23101 ccagatgtcg agcagcttga attgcagggc tatcgggtca ttagcggatt attagagatt
    23161 tatcgtcctt tattaagcct gtcgttatcc gactttactg aactggtaga aaaagaacgg
    23221 gtgaaacgtt tccctattga atcgcgctta ttccacaaac tctcgacgcg ccatcggctg
    23281 gcctatgtcg aggctgtcag taaattaccg tcagattctc ctgagtttcc gctatgggaa
    23341 tattattacc gttgccgcct gctgcaggat tatatcagcg gtatgaccga cctctatgcg
    23401 tgggatgaat accgacgtct gatggccgta gaacaataac caggcttttg taaagacgaa
    23461 caataaattt ttaccttttg cagaaacttt agttcggaac ttcgcgctat aaaacgaatc
    23521 tgaagaacac agaaattttg cgttatctgt taatcgagac tgaaatacat gaaaaaaacc
    23581 acattagcac tgagtgcact ggctctgagt ttaggtttgg cgttatctcc gctctctgca
    23641 acggcggctg agacttcttc agcaacgaca gcccagcaga tgccaagcct tgcaccgatg
    23701 ctcgaaaagg tgatgccttc agtggtcagc attaacgtag aaggtagcac aaccgttaat
    23761 acgccgcgta tgccgcgtaa tttccagcag ttcttcggtg atgattctcc gttctgccag
    23821 gaaggttctc cgttccagag ctctccgttc tgccagggtg gccagggcgg taatggtggc
    23881 ggccagcaac agaaattcat ggcgctgggt tccggcgtca tcattgatgc cgataaaggc
    23941 tatgtcgtca ccaacaacca cgttgttgat aacgcgacgg tgattaaagt ccagctgagc
    24001 gatggccgta agttcgacgc gaagatggtc ggcaaagatc cgcgctctga tatcgcgctg
    24061 atccaaatcc agaacccgaa aaacctgacc gcaattaaga tggcggattc tgatgcactg
    24121 cgcgtgggtg attacaccgt agcgattggt aacccgtttg gtctgggcga gacggtaact
    24181 tccgggattg tctctgcgct ggggcgtagc ggcctgaatg ctgaaaacta cgaaaacttc
    24241 atccagaccg atgcagcgat caaccgtggt aactccggtg gtgcgctggt taacctgaac
    24301 ggcgaactga tcggtatcaa caccgcgatc ctcgcaccgg acggcggcaa catcggtatc
    24361 ggttttgcaa tcccgagcaa catggtgaaa aacctgacct cgcagatggt ggaatacggc
    24421 caggtgaaac gcggtgagct gggtattatg gggactgagc tgaactccga actggcgaaa
    24481 gcgatgaaag ttgacgccca gcgcggtgct ttcgtaagcc aggttctgcc taattcctcc
    24541 gctgcaaaag cgggcattaa agcgggtgat gtgatcacct cactgaacgg taagccgatc
    24601 agcagctttg ccgcactgcg tgctcaggtg ggtactatgc cggtgggcag caaactgacc
    24661 ctgggcttac tgcgcgacgg taagcaggtt aacgtgaacc tggaactgca gcagagcagc
    24721 cagaatcagg ttgattccag ctccatcttc aacggcattg aaggcgctga gatgagcaac
    24781 aaaggcaaag atcagggcgt ggtagtgaac aacgtgaaaa cgggcactcc ggctgcgcag
    24841 atcggcctga agaaaggtga tgtgattatt ggcgcgaacc agcaggcagt gaaaaacatc
    24901 gctgaactgc gtaaagttct cgacagcaaa ccgtctgtgc tggcactgaa cattcagcgc
    24961 ggcgacagca ccatctacct gttaatgcag taatctcact caaccccttc ctgaaaacgg
    25021 gaaggggttc tccttacaat ctgtgaactt caccacaact ccatacatct tcatcatcct
    25081 ttaggcattt gcacaatgcc gtacgttacg tacttcctta tgctaagccg tgcataacgg
    25141 aggacttatg gctggctggc atcttgatac caaaatggcg caggatatcg tggcacgtac
    25201 catgcgcatc atcgatacca atatcaacgt aatggatgcc cgtgggcgaa ttatcggcag
    25261 cggcgatcgt gagcgtattg gtgaattgca cgaaggtgca ttgctggtac tttcacaggg
    25321 acgagtcgtc gatatcgatg acgcggtggc acgtcatctg cacggtgtgc ggcaagggat
    25381 taatctaccg ttacggcttg aaggtgaaat tgtcggcgta attggcctga caggtgaacc
    25441 agagaatctg cgtaaatatg gcgaactggt ctgcatgacg gctgaaatga tgctggaaca
    25501 gtcgcggttg atgcacttgt tggcgcagga tagccgtttg cgggaggaac tggtgatgaa
    25561 cctgattcag gcagaggaga atactcccgc acttactgaa tgggcgcaac ggctggggat
    25621 cgatctcaat caaccgcgag tggtggctat tgttgaggtc gacagcggtc agcttggtgt
    25681 ggacagcgca atggcggagt tacaacaact gcaaaacgcg ctgactacgc ccgagcgtaa
    25741 taatctggtg gcgattgtct cgctaaccga aatggtggtg ttgaaaccgg cgttgaactc
    25801 ttttgggcgc tgggatgcag aagatcatcg taagcgagtt gaacaactga ttacccgcat
    25861 gaaagagtac ggccggctgc gttttcgcgt ttcactgggc aactatttta ccggtcctgg
    25921 cagtattgcc cgatcctatc gtacggcgaa aacgacgatg gtggtgggta aacagcggat
    25981 gccagaaagt cgctgctatt tttatcagga tctgatgtta cctgtgttac tcgacagttt
    26041 gcgtggcgac tggcaggcca acgaactggc gcgaccgctg gcgcggctga aagcgatgga
    26101 caataacggc ttgctgcgac gaacgctggc ggcgtggttt cgtcacaatg tgcaaccgct
    26161 ggcaacgtca aaggcgttgt ttatttatcg taataccctg gagtatcggc ttaatcgtat
    26221 atcggaactg accgggcttg atttgggcaa ttttgatgac aggttgctgc tgtatgtggc
    26281 gttacaactg gatgaagagc ggtaggttat gcgttaaggt tgaggcggcg ctgtctcatt
    26341 tccctcagcc cgaaggggag aggggaatgc ccgaattaac cgtcaattcg ggattattta
    26401 cgcgttagtt tttccagatc ggcttcaatc tcgctgatct tattggttac gactgactcc
    26461 aggtgacgta agtcgtcgag gatcttacgc ttaagatcaa cttcgctgcg gtcacgctgg
    26521 cagatttgat caagctcatc aatgatatac cgtagattcg ggctgatttc ctggacttct
    26581 ttataaccct gacccacacc atcagcgacg accgttttac gctgacgcgg atatttaaac
    26641 ttaacgctct tggcgaaaaa ctcgcctttg tctttctgga aatagatttt cagaatatcg
    26701 ttgttggctt cctgccggag gctgtaacga tcaatttctt caggattggt aatacccaga
    26761 cttttcagat tgtcgtacat agcgttacct caaaatgagt cagtaaattg tgcttatttt
    26821 agcatttggc ctagcccgcc cggctcgact gtgatcgcag aaagcctttt tcttccagac
    26881 ttctatcatg gcgtaaaaat caaaaaatta cctgctttat tgtggtgata aaattcacgt
    26941 tctacaccag ttccagcatc gttacttccg ggcggcagtt cagacgcaaa ccatacaaac
    27001 tgcccacgcc acgggttgtg tagatgtgtc gttcgccaaa ggcatttaat ccggcgacgt
    27061 aacgtttatc ttcgacaggg gcaaaaggtt caccaaccaa cggtacgcgc agttgcccgc
    27121 cgtgagtatg gccgcacagc atcagatccc agggttcatc acgcatgact tctttgctgt
    27181 cgggattatg tgccagcatc agtcttggca gatttgcttc gctggcggga ggtggtttgc
    27241 attgtccggc ccataagtca ccggtgccta ccagttcgaa ttgcctgttc ggcgtggcga
    27301 tcaccgtagc ctggttaaac aacaccgtga tgcccactga tttcaacgtc tcgccaatta
    27361 agtgattttt ttctgtacca acagggcgat cgtggttgcc gaagcaggca aacgtcggcg
    27421 cacattcggc aaggggggag aggacatcac taaacgccga aaaattcagc ggcatatcaa
    27481 ataatacgta atcgccgccc agtaatatca aatcgggctt ttgttctatg ccaagagcaa
    27541 tcgcgtcaga aatcaggctt aaaggaacaa aacgagagta atggagatcg gccagaaaaa
    27601 gaattttgaa tggtgctgcg ttgtctttaa aaaaggcgag gcggtgacgg attaattcaa
    27661 accagcctgg ctcccagtaa tgcatataac cgaaacctga gctcgtggct atcgtcgcgg
    27721 cagtagcctg caacaatcgg cggcgtgaaa tcatcgctca tccctgcaaa aaaaatcggg
    27781 cagcgtcgtg ctgcccgtgt gcatactttt agtcgatggt acgcagcagt tcgttaatgc
    27841 cgactttgcc gcgagttttc gcgtcaactt tcttaacgat aaccgcacag tagaggctgt
    27901 atttgccatc ttttgacggc agattacctg aaacaaccac agaccccgcc ggaacgtgac
    27961 cgtagtggat ttcgccggtt tcacggtcgt aaatacgggt gctctgacca atgtatacgc
    28021 ccatggaaat gacggaacct tcttcgacaa tcaccccttc aactacttca gagcgtgcgc
    28081 caatgaagca gttatcttca atgatggttg ggttagcctg cagcggttcc agcacgccgc
    28141 cgatgcccac gccaccggaa aggtggacgt ttttaccaat ctgcgcacaa gaaccgacgg
    28201 tcgcccaggt atcaaccatg gtgccttcat caacatatgc gccgatgttg acgtaagacg
    28261 gcatcagcac ggtgttacgg gcaataaacg caccctgacg taccgccgct ggtggcacaa
    28321 cgcggaagcc ttctttctgg aaacgtgctt cgtcgtagtc ggcgaatttc attggcactt
    28381 tgtcgaagta gcggctttct gccccttcga tcacctgatt atcattaata cggaaagaga
    28441 gcagcaccgc ttttttcaac cactgatgcg tcacccactg accgtcaatt ttttccgcta
    28501 cacgcagtgc accggaatcc agcagggcga tcacctgatt taccgcttcg cgggtaacgg
    28561 tgtctgcgtt ggttggcgtg atctcggcac ggcgttcaaa agcggtttca ataatgttct
    28621 gtaactgctg cattgttaaa ctcttttcat atcagtaaac acatcaccct ttatcgtttg
    28681 gattgagggc ctctgtcaac cgctgatgca cttcctgctg caactcgtta ttaagcgcac
    28741 gccggtcagc ggtggcaatt atgaataaat cttctactcg ctcgccaatg gttgtaattc
    28801 gggcaccatg aagcgaaatt cccagatcgg caaaaatttt cccgactctc gccagcagtc
    28861 ctggctggtc gagcgcgata agctcaagga acgatttgcg gtcggtatgg gtcggcaaaa
    28921 acgttacttc ggtttcaaca gtaaaatggc gtaatttggc gggttggcga cggggctgcg
    28981 gtggctgcca gctactttgc gtcagtactt gctccagacc aaaccgaata acctcatgac
    29041 gatctgcgga cagcgggctg ccatcgggtt ccagcacgat aaaggtatcc atcgccatac
    29101 cgtcgcgagt ggtgaaaatt tgtgcgtcgt gaacacttaa attgcggcgg tctaattcgg
    29161 cacagacggc ggcaaacaga taggggcggt ccgggctcca gataaaaatc tcggtgcctc
    29221 cacgcgtagc ctgcgggcta agcaatacca gtggtttgct taaatcatgc tgtaataaat
    29281 ggcgggcatg ccaggccagt tgatttgggc tatggcggac aaaatagtta gcacgacagc
    29341 gtgaccaaat ttggtgcagc gcctcttcgt cgatgttatc catgcgcagt agtgccagtg
    29401 cctggagttg gtgatggcga acccgttcgc gcatatccgg cgtgttttgc attccgcgtc
    29461 gtagctgctt ttcggtggca aaatagagtt cgcgcaacag actttgcttc cagctattcc
    29521 acagcgtttc gttggtggcg caaatgtcag ccactgtcag gcataccaga tagcgcagac
    29581 gattttccgt ttgaacttct tcggcaaact gcttgatgac ttcagggtcc tgaatatcgc
    29641 ggcgttgtgc tgtcaccgac atcagcaagt gctggcgaac cagccaggca accagctgtg
    29701 tttcgcgtga gttcagcccg tggagttctg caaaatgcac tacatcctga gcaccgagaa
    29761 tggagtggtc gccgccgcgt cctttggcga tatcgtgaaa cagcgcggcg atgaaaatca
    29821 gctcagttga cggcaggcgc ggccagacgt ccacacacaa cggatggcgc tggcgcgttt
    29881 cttcactggc aaaactctcc agtttcagca tcacgcggat agtatgttca tccaccgtgt
    29941 aggcgtggaa cagatcaaac tgcatctgcc cgacgatatg cgaccattgc ggcatatacg
    30001 cgccgagcac gctatggcga tgcattggca atagcccgcg ccgcaccgct ccggggtgac
    30061 gcagaatgct caaaaacagt tttcgtgctt ccggaatatt acacagcggt tgttgcagat
    30121 ggcgacgggc atggcgtaac tggcgcagcg tggtggagta aatgccggtg atcgcactgt
    30181 tgcgcaccat ggtgtagaac atacgcaaga tggcttccgg ctggcgcata aatagtgttt
    30241 catcacgcag gtcgattagc gtaccgcgta gctgaaactc atcgtcgatt ggacgtggtt
    30301 tttcgtcggc aggaagggcg aggatggctt catcgaacag ttgcagcagc atctggttga
    30361 gttcactgac gcggcgtgta acgcggaagt aatccttcat catccgctcg accggttcgt
    30421 tgccttcacc actgtaattc agacgctgag cgacgctaag ttggcgatcg aataacaggc
    30481 gattatcgta acggctgacg accagatgca gggcaaagcg aatacgccac agtatatgca
    30541 gacattcgtt taattccgcc cgctccgctg aggttaagaa gccaaaccca accatttcat
    30601 ccagcgatgt tgcgccaaaa tgacggcggg ccacccattg cagagtgtgg atatcgcgca
    30661 agccgccagg gctacttttg atgtctggtt caagattgta gctggtgccg tggtaacgct
    30721 gatggcgctg gttctgttct tcaactttcg ccgcgtagaa cttgtcggaa ggccagaatc
    30781 cttcgctaaa aatatgtttt tgcagttcga ggaagagcgc aacatcgcca attaataagc
    30841 gggattcgat taaattggtg gcgacggtta aatccgataa cccttccagc atgcactctt
    30901 caagcgtgcg cacactatga ccgacttcca gctttacatc ccagagcagc gttaacagct
    30961 cgcccacttt ttgcgcctga tcgtccggga gctttttacg gcttaaaatc agtaaatcga
    31021 tgtcagaaag tggatgcagc tcgccacgac cgtagccacc gacggcgacc aatgccaggt
    31081 cggcaatctg gctgaatccc gcttcaatcc ataatcgttg caggagctgg tcgataaact
    31141 cggtgcgcgc ctcaatcaac tgttctgcag agatcccatt gtcaaaggca tcacccagcc
    31201 aacgctggaa agtatcgata tgggctttta tcccaccgac ggttaattca tcacggggcc
    31261 agacgcatgg attttgcggt tgaccgggca gggtggggag agcggtgttt gcgtactgtt
    31321 ctggaagggt attcattgtg cgccactcat aagattaaat tatcgcatta aaaaagccgg
    31381 cattcgccgg cttcatctta ttcgtcgtgc gagattatcg ccgggatggt gtcatccttg
    31441 cgtagcgtca gaatttcgca gccgttatca gtcaccacaa tagtatgctc atattgtgca
    31501 gacaagctgc gatctttggt ttttaccgtc cagccatctt tcatggtgcg gatctctttt
    31561 ttacccgcgt tgaccattgg ctcgatggtg aacgtcatcc caggtttcag tacgacgttg
    31621 gtttcacggg agtcatagtg cagcacctgc ggttcttcat ggaagccgcg accaataccg
    31681 tgtccgcaat attcacgaac gacggagaag ccttctgctt cgacaaactt ctgaatcgcc
    31741 gcaccgattt cgcgcaggtt aatgcctggt ttgaccatgc gcagcgccag gtacaggctt
    31801 tcttgcgtga tgcggcacag acgttcgccc atgatggtcg gcttaccgac gataaacatt
    31861 ttcgaggtat cgccgtggaa accatctttg attacggtga catcaatgtt aacgatatcg
    31921 ccatctttca gcagcttcgc atcgtccggg ataccgtggc acaccacttc attaatagag
    31981 atgcaaacgg atttcggata gccgtgatag ccgaggcagg cagaaaccgc gtgttgttca
    32041 ttaacaatgt aatcattaca gatgcgatcc agctcgccgg tgctgacgcc cggtttaaca
    32101 tacggttcga tcatctccag cacttcggca gccagtcggc cagcgacgcg cattttttcg
    32161 atatcttctg gggtcttgat tgagatagcc attaattctg tccatcagcg tcggtgatac
    32221 cgacaatata tatgtaagtg ccgtcaatgg tatcacaccc gggcaaattg agaatcattc
    32281 tgaatttcgc caaacgtgcc actgaacgtt ttctataata gaaaattcaa cgtctgatgc
    32341 tgtacacagc gccaacaatt attggtgtcc atgacgtatt tgtggtataa agcgcgccgg
    32401 acttccgatc catttcgtat acacagactg gacggaagcg acaatctcac tttgtgtaac
    32461 aacacacacg tatcggcaca tattccgggg tgccctttgg ggtcggtaat atgggatacg
    32521 tggaggcata accccaactt ttatatagag gttttaatca tggcaactgt ttccatgcgc
    32581 gacatgctca aggctggtgt tcacttcggt caccagaccc gttactggaa cccgaaaatg
    32641 aagccgttca tcttcggtgc gcgtaacaaa gttcacatca tcaaccttga gaaaactgta
    32701 ccgatgttca acgaagctct ggctgaactg aacaagattg cttctcgcaa aggtaaaatc
    32761 cttttcgttg gtactaaacg cgctgcaagc gaagcggtga aagacgctgc tctgagctgc
    32821 gaccagttct tcgtgaacca tcgctggctg ggcggtatgc tgactaactg gaaaaccgtt
    32881 cgtcagtcca tcaaacgtct gaaagacctg gaaactcagt ctcaggacgg tactttcgaa
    32941 aagctgacca agaaagaagc gctgatgcgc actcgtgagc tggagaaact ggaaaacagc
    33001 ctgggcggta tcaaagacat gggcggtctg ccggacgctc tgtttgtaat cgatgctgac
    33061 cacgaacaca ttgctatcaa agaagcaaac aacctgggta ttccggtatt tgctatcgtt
    33121 gataccaact ctgatccgga cggtgttgac ttcgttatcc cgggtaacga cgacgcaatc
    33181 cgtgctgtga ccctgtacct gggcgctgta gctgcaaccg tacgtgaagg ccgttctcag
    33241 gatctggctt cccaggcgga agaaagcttc gtagaagctg agtaataagg cttgataact
    33301 cccccaaaat agttcgagtt gcagaaaggc ggcaagctcg agaattcccg ggagcttaca
    33361 tcagtaagtg accgggatga gcgagcgaag ataacgcatc tgcggcgcga aatatgaagg
    33421 gggagagccc ttatagacca ggtagtacac gtttggttag ggggcctgca tatggccccc
    33481 tttttcactt ttatatctgt gcggtttaat gccgggcaga tcacatctcc gaggatttta
    33541 gaatggctga aattaccgca tccctggtaa aagagctgcg tgagcgtact ggcgcaggca
    33601 tgatggattg caaaaaagca ctgactgaag ctaacggcga catcgagctg gcaatcgaaa
    33661 acatgcgtaa gtccggtgct attaaagcag cgaaaaaagc aggcaacgtt gctgctgacg
    33721 gcgtgatcaa aaccaaaatc gacggcaact acggcatcat tctggaagtt aactgccaga
    33781 ctgacttcgt tgcaaaagac gctggtttcc aggcgttcgc agacaaagtt ctggacgcag
    33841 ctgttgctgg caaaatcact gacgttgaag ttctgaaagc acagttcgaa gaagaacgtg
    33901 ttgcgctggt agcgaaaatt ggtgaaaaca tcaacattcg ccgcgttgct gcgctggaag
    33961 gcgacgttct gggttcttat cagcacggtg cgcgtatcgg cgttctggtt gctgctaaag
    34021 gcgctgacga agagctggtt aaacacatcg ctatgcacgt tgctgcaagc aagccagaat
    34081 tcatcaaacc agaagacgta tccgctgaag tggtagaaaa agaataccag gtacagctgg
    34141 atatagcgat gcagtctggt aagccgaaag aaatcgcaga gaaaatggtt gaaggccgca
    34201 tgaagaaatt caccggcgaa gtttctctga ccggtcagcc gttcgttatg gaaccaagca
    34261 aaactgttgg tcagctgctg aaagagcata acgctgaagt gactggcttc atccgcttcg
    34321 aagtgggtga aggcatcgag aaagttgaga ctgactttgc agcagaagtt gctgcgatgt
    34381 ccaagcagtc ttaattatca aaaaggagcc gcctgagggc ggcttctttt tgtgcccatc
    34441 ttgtaaattc agctaaccct tgtggggctg cgctgaaaag cgacgtacaa tgtcgctagt
    34501 attaattcat ttcaatcgtt gacagtctca ggaaagaaac atggctacca atgcaaaacc
    34561 cgtctataaa cgcattctgc ttaagttgag tggcgaagct ctgcagggca ctgaaggctt
    34621 cggtattgat gcaagcatac tggatcgtat ggctcaggaa atcaaagaac tggttgaact
    34681 gggtattcag gttggtgtgg tgattggtgg gggtaacctg ttccgtggcg ctggtctggc
    34741 gaaagcgggt atgaaccgcg ttgtgggcga ccacatgggg atgctggcga ccgtaatgaa
    34801 cggcctggca atgcgtgatg cactgcaccg cgcctatgtg aacgctcgtc tgatgtccgc
    34861 tattccattg aatggcgtgt gcgacagcta cagctgggca gaagctatca gcctgttgcg
    34921 caacaaccgt gtggtgatcc tctccgccgg tacaggtaac ccgttcttta ccaccgactc
    34981 agcagcttgc ttgcgtggta tcgaaattga agccgatgtg gtgctgaaag caaccaaagt
    35041 tgacggcgtg tttaccgctg atccggcgaa agatccaacc gcaaccatgt acgagcaact
    35101 gacttacagc gaagtgctgg aaaaagagct gaaagtcatg gacctggcgg ccttcacgct
    35161 ggctcgtgac cataaattac cgattcgtgt tttcaatatg aacaaaccgg gtgcgctgcg
    35221 ccgtgtggta atgggtgaaa aagaagggac tttaatcacg gaataattcc cgtgatggat
    35281 aaataagggt aagattccgc gtaagtatcg cgggggcgta agtctggtta taaggcgtta
    35341 ttgttgcagg cagtttggtc acggccagcg cgcagcaacc ggagcgtaca aaagtacgtg
    35401 aggatggcga gcactgctcg gggccaaaat ggcaaataaa atagcctaat aatccagacg
    35461 attacccgta atatgtttaa tcagggctat acttagcaca cttccactgt gtgtgactgt
    35521 ctggtctgac tgagacaagt tttcaaggat tcgtaacgtg attagcgata tcagaaaaga
    35581 tgctgaagta cgcatggaca aatgcgtaga agcgttcaaa acccaaatca gcaaaatacg
    35641 cacgggtcgt gcttctccca gcctgctgga tggcattgtc gtagaatatt acggcacgcc
    35701 gacgccgctg cgtcagctgg caagcgtaac ggtagaagat tcccgtactc tgaaaatcaa
    35761 cgtgtttgat cgttcaatgt ctccggccgt tgaaaaagcg attatggcgt ccgatcttgg
    35821 cctgaacccg aactctgcgg gtagcgacat ccgtgttccg ctgccgccgc tgacggaaga
    35881 acgtcgtaaa gatctgacca aaatcgttcg tggtgaagca gaacaagcgc gtgttgcagt
    35941 acgtaacgtg cgtcgtgacg cgaacgacaa agtgaaagca ctgttgaaag ataaagagat
    36001 cagcgaagac gacgatcgcc gttctcagga cgatgtacag aaactgactg atgctgcaat
    36061 caagaaaatt gaagcggcgc tggcagacaa agaagcagaa ctgatgcagt tctgatttct
    36121 tgaacgacaa aaacgccgct cagtagatcc ttgcggatcg gctggcggcg ttttgctttt
    36181 tattctgtct caactctgga tgtttcatga agcaactcac cattctgggc tcgaccggct
    36241 cgattggttg cagcacgctg gacgtggtgc gccataatcc cgaacacttc cgcgtagttg
    36301 cgctggtggc aggcaaaaat gtcactcgca tggtagaaca gtgcctggaa ttctctcccc
    36361 gctatgccgt aatggacgat gaagcgagtg cgaaacttct taaaacgatg ctacagcaac
    36421 agggtagccg caccgaagtc ttaagtgggc aacaagtcgc ttgcgatatg gcagcgcttg
    36481 aggatgttga tcaggtgatg gcagccattg ttggcgctgc tgggctgtta cctacgcttg
    36541 ctgcgatccg cgcgggtaaa accattttgc tggccaataa agaatcactg gttacctgcg
    36601 gacgtctgtt tatggacgcc gtaaagcaga gcaaagcgca attgttaccg gtcgatagcg
    36661 aacataacgc catttttcag agtttaccgc aacctatcca gcataatctg ggatacgctg
    36721 accttgagca aaatggcgtg gtgtccattt tacttaccgg gtctggtggc cctttccgtg
    36781 agacgccatt gcgcgatttg gcaacaatga cgccggatca agcctgccgt catccgaact
    36841 ggtcgatggg gcgtaaaatt tctgtcgatt cggctaccat gatgaacaaa ggtctggaat
    36901 acattgaagc gcgttggcta tttaacgcca gcgccagcca gatggaagtg ctgattcacc
    36961 cgcagtcagt gattcactca atggtgcgct atcaggacgg cagtgttctg gcgcagctgg
    37021 gggaaccgga tatgcgtacg ccaattgccc acaccatggc atggccgaat cgcgtgaact
    37081 ctggcgtgaa gccgctcgat ttttgcaaac taagtgcgtt gacatttgcc gcaccggatt
    37141 atgatcgtta tccatgcctg aaactggcga tggaggcgtt cgaacaaggt caggcagcga
    37201 cgacagcatt gaatgccgca aacgaaatca ccgttgctgc ttttcttgcg caacaaatcc
    37261 gctttacgga tatcgctgcg ttgaatttat cggtactgga aaaaatggat atgcgcgaac
    37321 cacaatgtgt ggacgatgtg ttatctgttg atgcgaacgc gcgtgaagtc gccagaaaag
    37381 aggtgatgcg tctcgcaagc tgaggataat ccggctacag agagtctcgc tatttgttag
    37441 cgtagggctt cagtgatata gtctgcgcca tctgatcgta agtagttggc tttataaggt
    37501 caggtatgcc gtggttttgc acggcttttt ttgtataggc ttcagtattc ctgagtaccg
    37561 taaaccctgt cagggaataa aaaacgcgtg atgttgtctg ctactcaacc acttagcgaa
    37621 aaattgccag cgcatggctg ccgtcatgtt gcgatcatta tggacggcaa tggtcgctgg
    37681 gcaaaaaagc aagggaagat tcgtgccttt gggcataaag ccggggcaaa atccgtccgc
    37741 cgggctgtct cttttgcggc caacaacggt attgaggcgt taacgctgta tgcctttagt
    37801 agtgaaaact ggaaccgacc agcgcaggaa gtcagtgcgt taatggaact gtttgtgtgg
    37861 gcgctcgata gcgaagtaaa aagtctgcac cgacataacg tgcgtctgcg tattattggc
    37921 gataccagtc gctttaactc gcgtttgcaa gaatgtattc gtaaatctga agcgctaaca
    37981 gccgggaata ccggtctgac gctgaatatt gcggcgaact acggtggacg ttgggatata
    38041 gtccagggag tcaggcaact ggctgaaaag gtgcagcaag gaaacctgca accagatcag
    38101 atagatgaag agatgctaaa ccagcatgtc tgtatgcatg aactggcccc tgtagattta
    38161 gtaattagga ctggggggga gcatcgcatt agtaactttt tgctttggca aattgcctat
    38221 gccgaacttt actttataga tgttctctgg cccgatttcg atgaacaaga ctttgaaggg
    38281 gcgttaaatg cctttgctaa tcgagagcgt cgtttcggcg gcaccgagcc cggtgatgaa
    38341 acagcctgat ggggggcgct tttgctgaag tatcgcctga tatctgcttt tgtgttaata
    38401 cccgtcgtca tcgcggcgtt gtttctgttg ccgccggtgg ggttcgccat tgtaacgctg
    38461 gtggtctgca tgctggcagc gtgggaatgg ggacagctta gcggttttac cactcgttcg
    38521 cagcgagtat ggttggcggt gttatgcggg ttattgttgg cgctgatgct ttttctgttg
    38581 ccggaatatc accgaaatat tcatcaaccg ctggttgaaa tctcactttg ggcttcgctg
    38641 ggttggtgga ttgtctcgct attgctggtg ctgttttacc caggttccgc agcaatctgg
    38701 cgtaactcta aaacattgcg ccttattttt ggcgtgctaa ccattgttcc cttcttctgg
    38761 ggcatgctgg cgttacgggc ctggcactat gacgagaatc attacagtgg cgcaatatgg
    38821 ctgctctatg tcatgatcct ggtatggggc gctgactccg gcgcatatat gtttggcaaa
    38881 ttgtttggta aacataagct ggcaccgaag gtttctccgg gtaaaacctg gcaaggcttt
    38941 ataggtggac tcgctactgc agcggtaatc tcatggggtt atggcatgtg ggcgaatctc
    39001 gacgtcgctc ccgtcacctt actcatttgc tctattgtcg cagcgttagc ctcagtgctc
    39061 ggcgatctga ccgagagtat gtttaagcgt gaagcaggaa ttaaggacag cggtcattta
    39121 attccaggac acggtggtat tttagatcgt attgatagcc tgacggctgc ggtaccggtc
    39181 tttgcttgct tgttgttact ggtattcagg acgctttaac ggaaggtaat atgctgagtt
    39241 ttctctggga tttggcttcg ttcatcgttg cactgggtgt acttatcact gtgcatgaat
    39301 ttggtcattt ctgggttgcc cggcgttgtg gtgttcgcgt tgagcgtttc tcaatagggt
    39361 ttggtaaggc gctctggcgg cgaactgata agctcggcac cgaatatgtt atcgccctga
    39421 tcccgctggg cggttatgtc aaaatgctgg atgagcgcgc agaaccggtc gttccggaac
    39481 tccgccacca tgccttcaat aataaatctg ttggccaacg agcggcgatt attgccgcag
    39541 gtccggttgc aaacttcatt tttgctatct ttgcctactg gctggttttt attattggtg
    39601 tgcctggcgt acgtccggtg gttggtgaaa tagcagccaa ttcgatagct gcggaagcac
    39661 aaattgcacc aggtacggaa ctaaaagccg tagatggtat cgaaacgcct gattgggatg
    39721 ccgtgcgttt gcagttggtc gataaaattg gcgatgaaag caccaccatt acagtagcgc
    39781 catttggcag cgaccaacgg cgggatgtaa agctcgattt acgtcactgg gcgtttgagc
    39841 ctgataaaga agatccggta tcttcgctgg ggattcgtcc tcgtgggctg caaattgaac
    39901 ctgtactgga aaatgtgcag ccaaactcgg cggcaagcaa ggcaggtttg caagcaggcg
    39961 acaggatcgt taaagtcgat ggtcagccct taacgcagtg ggtgaccttt gtgatgcttg
    40021 tccgggataa cccggggaaa tccttagcgt tagaaatcga aaggcaggga agtctcttgt
    40081 ctttgacatt aatcccggag agtaaaccgg gtaatggtaa agcgattggt tttgtcggta
    40141 ttgagccgaa agtcattcct ttgccagatg agtataaagt tgtacgccag tatgggccgt
    40201 tcaacgccat cgtcgaagcc acggacaaaa cgtggcagct gatgaagctg acggtcagta
    40261 tgctgggaaa attgatcacc ggtgatgtga aactgaacaa cctcagtggg ccgatctcta
    40321 tcgccaaggg ggctgggatg acagcggaac tcggggtagt ttattacctg ccgtttcttg
    40381 cgcttattag cgtgaactta gggataatta acctgtttcc gttgcccgta cttgacgggg
    40441 cgcatctgct gttccttgcg atcgaaaaga tcaagggcgg accggtatcc gagcgggttc
    40501 aagacttttg ttatcgcatt ggctcgattc tgctggtgct gttaatgggg cttgcacttt
    40561 tcaatgattt ctctcggtta tgagagttag ttaggaagaa cgcataataa cgatggcgat
    40621 gaaaaagttg ctcatagcgt cgctgctgtt tagcagcgcc accgtatacg gtgctgaagg
    40681 gttcgtagtg aaagatattc atttcgaagg ccttcagcgt gtcgccgttg gtgcggccct
    40741 cctcagtatg ccggtgcgta caggcgacac ggttaatgat gaagatatca gtaataccat
    40801 tcgcgctctg tttgctaccg gcaactttga ggatgttcgc gtccttcgtg atggtgatac
    40861 ccttctggtt caggtaaaag aacgtccgac cattgccagc attactttct ccggtaacaa
    40921 atcggtgaaa gatgacatgc tgaagcaaaa cctcgaggct tctggtgtgc gtgtgggcga
    40981 atccctcgat cgcaccacca ttgccgatat cgagaaaggt ctggaagact tctactacag
    41041 cgtcggtaaa tatagcgcca gcgtaaaagc tgtcgtgacc ccgctgccgc gcaaccgtgt
    41101 tgacctaaaa ctggtgttcc aggaaggtgt gtcagctgaa atccagcaaa ttaacattgt
    41161 tggtaaccat gctttcacca ccgacgaact gatctctcat ttccaactgc gtgacgaagt
    41221 gccgtggtgg aacgtggtag gcgatcgtaa ataccagaaa cagaaactgg cgggcgacct
    41281 tgaaaccctg cgcagctact atctggatcg cggttatgcc cgtttcaaca tcgactctac
    41341 ccaggtcagt ctgacgccag ataaaaaagg tatttacgtc acggtgaaca ttaccgaagg
    41401 cgatcagtac aagctttctg gcgttgaagt gagcggcaac cttgccgggc actccgctga
    41461 aattgagcag ctgactaaga tcgagccggg cgagctgtat aacggcacca aagtgaccaa
    41521 gatggaagat gacatcaaaa agcttctcgg tcgctatggt tatgcctatc cgcgcgtaca
    41581 gtcgatgcct gaaattaacg atgccgacaa aaccgttaaa ttacgcgtga acgttgatgc
    41641 gggtaaccgt ttctacgtgc gtaagatccg ttttgaaggt aacgatacct cgaaagatgc
    41701 cgtcctgcgt cgcgaaatgc gtcagatgga aggtgcatgg ctgggaagcg atctggtcga
    41761 tcagggtaag gagcgtctga atcgtctggg cttctttgaa actgtcgata ccgataccca
    41821 acgtgttccg ggtagcccgg accaagtaga tgtagtctac aaggtgaaag aacgtaacac
    41881 cggtagcttc aacttcggga ttggttacgg tactgaaagt ggcgtgagct tccaggctgg
    41941 cgtgcaacag gataactggt taggtacagg ttatgctgtt ggtatcaacg ggaccaaaaa
    42001 cgattaccag acctatgctg aattgtcggt aaccaacccg tacttcactg tagatggtgt
    42061 aagcctcggt ggtcgtctct tctataatga cttccaggca gatgacgccg acctgtccga
    42121 ctataccaac aagagttatg gtacagacgt gacgttgggc ttcccgatca acgaatataa
    42181 ctcgctgcgt gcaggtctgg gttatgtaca taactccctg tccaacatgc agcctcaggt
    42241 tgcgatgtgg cgttatctgt actctatggg tgaacatccg agcacctctg atcaggataa
    42301 cagcttcaaa acggacgact tcacgttcaa ctatggttgg acctataaca agcttgaccg
    42361 tggttacttc ccgacagatg gttcacgtgt caacctgacc ggtaaagtga ccattcctgg
    42421 atcggataac gaatactaca aagtgacgtt agacacggcg acttatgtgc cgatcgatga
    42481 cgatcacaaa tgggttgttc tggggcgtac ccgctggggt tatggtgatg gtttaggcgg
    42541 caaagagatg ccgttctacg agaacttcta tgccggtggt tccagcaccg tccgtggctt
    42601 ccagtccaat accattggtc cgaaagcagt ttacttcccg catcaggcca gtaattatga
    42661 tccggactat gattacgaat gtgcgactca ggacggcgcg aaagacctgt gtaaatcgga
    42721 tgatgctgta ggcggtaacg ccatggcggt tgccagcctc gagttcatca ccccgacgcc
    42781 gtttattagc gataagtatg ctaactcggt tcgtacttcc ttcttctggg atatgggtac
    42841 cgtttgggat acaaactggg attccagcca atattctgga tatccggact atagtgatcc
    42901 aagcaatatc cgtatgtctg cgggtatcgc attacaatgg atgtccccat tggggccgtt
    42961 ggtgttctcc tacgcccagc cgttcaaaaa gtacgatgga gacaaggcag aacagttcca
    43021 gtttaacatc ggtaaaacct ggtaagtgtt ctccacaaag gaatgtagtg gtagtgtagc
    43081 gatgacttta ggcgatcaat ataagatcgc cgggccacgc aaagaactgc accctccggt
    43141 gcaaatggga tggtaaggag tttattgtga aaaagtggtt attagcggca ggtctcggtt
    43201 tagcactggc aacttctgct caggcggctg acaaaattgc aatcgtcaat atgggcagcc
    43261 tgttccagca ggtagcgcag aaaaccggtg tttctaacac gctggaaaat gagttcaaag
    43321 gccgtgccag cgaactgcag cgtatggaaa ccgatctgca ggctaaaatg aaaaagctgc
    43381 agtccatgaa agcgggcagc gatcgcacta agctggaaaa agacgtgatg gctcagcgcc
    43441 agacttttgc tcagaaagcg caggcttttg agcaggatcg cgcacgtcgt tccaacgaag
    43501 aacgcggcaa actggttact cgtatccaga ctgctgtgaa atccgttgcc aacagccagg
    43561 atatcgatct ggttgttgat gcaaacgccg ttgcttacaa cagcagcgat gtaaaagaca
    43621 tcactgccga cgtactgaaa caggttaaat aagtaatgcc ttcaattcga ctggctgatt
    43681 tagcgcagca gttggatgca gaactacacg gtgatggcga tatcgtcatc accggcgttg
    43741 cgtccatgca atctgcacaa acaggtcaca ttacgttcat ggttaaccca aaataccgtg
    43801 agcatttagg cttgtgccag gcgtccgcgg ttgtcatgac ccaggacgat cttcctttcg
    43861 cgaaaagtgc cgcgctggta gtgaagaatc cctacctgac ttacgcgcgc atggcgcaaa
    43921 ttttagatac cacgccgcag cccgcgcaga acattgcacc cagtgcggtg atcgacgcga
    43981 cggcgaagct ggggaacaat gtatcgattg gcgctaacgc ggtgattgaa tccggcgttg
    44041 aactgggcga taacgtgatt atcggtgccg gttgcttcgt aggtaaaaac agcaaaatcg
    44101 gtgcaggttc gcgtctctgg gcgaacgtaa ccatttacca tgagatccag atcggtcaga
    44161 attgcctgat ccagtccgga acagtggtag gcgcagacgg ctttggttat gccaacgatc
    44221 gtggtaactg ggttaagatc ccacagattg gtcgcgtaat tattggcgat cgcgtggaga
    44281 tcggtgcctg cactaccatc gatcgcggcg cactggatga cactgttatt ggcaatggcg
    44341 tgatcattga taaccagtgc cagattgcac ataacgtcgt gattggcgac aatacggcgg
    44401 ttgccggtgg cgtcattatg gcgggcagcc tgaaaattgg tcgttactgc atgatcggcg
    44461 gagccagcgt aatcaacggg catatggaaa tatgcgacaa agtgacggtt acgggcatgg
    44521 gtatggtgat gcgtcccatc actgaaccag gcgtctattc ctcaggcatt ccgctgcaac
    44581 ccaacaaagt ctggcgcaaa accgctgcac tggtgatgaa cattgatgac atgagcaagc
    44641 gtctgaaatc gcttgagcgc aaggttaatc aacaagacta acgttccatc ttttgttcgc
    44701 caaactttac ggcctgtctc attcttacga ttgcggcagg ccgtgttatt attgtcgttt
    44761 cttatatttt gacaggaaga gtatcttgac tactaacact catactctgc agattgaaga
    44821 gattttagaa cttctgccgc accgtttccc gttcttactg gtggatcgcg tgctggattt
    44881 tgaagaaggt cgttttctgc gcgcagtaaa aaatgtctct gtcaatgagc cattcttcca
    44941 gggccatttc cctggaaaac cgattttccc gggtgtgctg attctggaag caatggcaca
    45001 ggcaacaggt attctggcgt ttaaaagcgt aggaaaactg gaaccgggtg agctgtacta
    45061 cttcgctggt attgacgaag cgcgcttcaa gcgcccggtc gtgcctggcg atcaaatgat
    45121 catggaagtc actttcgaaa aaacgcgccg cggcctgacc cgttttaaag gggttgctct
    45181 ggtcgatggt aaagtagttt gcgaagcaac gatgatgtgt gctcgtagcc gggaggcctg
    45241 atacgtgatt gataaatccg cctttgtgca tccaaccgcc attgtggaag agggcgcgtc
    45301 aattggcgcg aacgcacaca ttggtccttt ttgtatcgtt ggaccccatg tcggaattgg
    45361 tgagggtacc gtactgaaat ctcacgttgt cgtgaatggt catactaaaa ttggccgcga
    45421 taatgagatt tatcagttcg cctccatcgg cgaagttaac caggatctga aatatgctgg
    45481 cgaaccgacc cgtgtggaaa tcggcgatcg taaccgcatt cgcgaaagcg tcaccattca
    45541 tcgtggcaca gtccagggcg gtggattgac gaaggtgggc agcgacaact tactgatgat
    45601 caacgcgcac attgcgcacg attgtacggt aggtaaccgc tgtattctcg ccaacaacgc
    45661 aacgctggcg ggtcacgtat cggttgacga cttcgcgatc atcggcggca tgaccgcagt
    45721 ccatcagttc tgcatcattg gtgcgcacgt gatggttggc ggctgctccg gtgtggcgca
    45781 ggacgtccct ccttatgtca ttgcgcaggg taaccacgca acgccgttcg gtgtcaatat
    45841 cgaagggctg aagcgccgcg gattcagccg tgaggcgatt accgctatcc gcaatgcgta
    45901 taagctgatt tatcgtagcg gtaaaacgct cgatgaagtg aaaccggaaa ttgctgaact
    45961 ggcggaaaca tatccggaag tgaaagcctt taccgatttc ttttcacgct caacgcgcgg
    46021 tctgattcgt taatgactga acagcgtcca ttaacgattg ccctggtcgc cggagaaacc
    46081 tccggcgata tcctaggggc cggtttaatc cgcgctctga aagaacgtgt gcccaacgcc
    46141 cgctttgttg gtgttgccgg gccacgaatg caggctgaag gctgcgaagc ctggtacgaa
    46201 atggaagaac tggcggtgat gggcattgtt gaagtgctcg gtcgtctgcg tcgcttactg
    46261 catattcgtg ccgatctgac aaagcgtttt ggcgaactga agccagatgt ttttgttggt
    46321 attgatgcgc ctgacttcaa tattactctt gaaggtaacc tcaaaaagca gggtatcaaa
    46381 accattcatt acgtcagtcc gtcagtctgg gcgtggcgac agaaacgtgt tttcaaaata
    46441 ggcagagcca ccgatctggt gctcgcattt ctgcctttcg aaaaagcgtt ttatgacaaa
    46501 tacaacgtac cgtgccgctt tatcggtcat accatggctg atgccatgcc attagatcca
    46561 gataaaaatg ccgcccgtga tgtgctgggg atccctcacg atgcccactg cctggcgttg
    46621 ctaccgggga gccgtggtgc agaagttgaa atgcttagtg ccgatttcct gaaaacggcc
    46681 cagcttttgc gccagacata tccggatctc gaaatcgtgg tgccactggt gaatgccaaa
    46741 cgccgcgagc agtttgaacg catcaaagct gaagtcgcgc cagacttttc agttcatttg
    46801 ctggatggga tgggccgtga ggcgatggtc gccagcgatg cggcgctact ggcgtcgggt
    46861 acggcagccc tggagtgtat gctggcgaaa tgcccgatgg tggtgggata tcgcatgaag
    46921 ccttttacct tctggttggc gaagcggctg gtgaaaactg attatgtctc gctgccaaat
    46981 ctgctggcgg gcagagagtt agtcaaagag ttattgcagg aagagtgtga gccgcaaaaa
    47041 ctggctgcgg cgctgttacc gctgttggcg aacgggaaaa tcagccacgc gatgcacgat
    47101 accttccgtg aactgcatca gcagatccgc tgcaatgccg atgagcaggc ggcacaagcc
    47161 gttctggagt tagcacaatg atcgaatttg tttatccgca cacgcagctg gttgcgggtg
    47221 tggatgaagt cggacgcggg ccgttagttg gcgcggtcgt caccgctgcg gtgatccttg
    47281 acccggcgcg cccgattgcc gggctgaatg attccaaaaa gctgagcgaa aaacgccgtc
    47341 tggcgctctg tgaagagatc aaagagaaag cgttgagctg gagtctgggc cgcgcggaac
    47401 cccacgaaat cgacgagctg aacattcttc atgcgaccat gctggcgatg cagcgtgccg
    47461 tcgctgggct gcatattgcg ccggaatatg tgttgattga tggtaaccgc tgcccgaaat
    47521 taccgatgcc tgcgatggtt gtggtgaaag gcgatagccg cgtaccggaa atcagtgccg
    47581 cgtctatcct ggcgaaagtg acgcgtgacg ccgaaatggc ggcgctggat attgttttcc
    47641 cgcaatatgg ttttgcccaa cacaaagggt acccaaccgc ttttcatctg gaaaaactgg
    47701 ctgaacacgg cgcgaccgaa caccatcggc gcagctttgg gcctgtcaaa cgcgcactgg
    47761 gacttgcgtc ctgattcttg tgtcgagatt aagtaaaccg gaatctgaag atgtctgaac
    47821 cacgtttcgt acacctgcgg gtgcacagcg actactcgat gatcgatggc ctggccaaaa
    47881 ctgcaccgtt ggtaaaaaag gcggcggcgt tgggtatgcc agcactggcg atcaccgatt
    47941 tcaccaacct ttgtggtctg gtgaagttct acggagcggg acatggcgca gggattaagc
    48001 ctatcgtcgg ggcagatttt aacgtccagt gcgacctgct gggtgatgag ttaacccacc
    48061 tgacggtact ggcggcgaac aataccggct atcagaatct gacgttgctg atctcaaaag
    48121 cgtatcagcg cgggtacggt gccgctgggc cgatcatcga tcgcgactgg cttatcgaat
    48181 taaatgaagg gttgatcctt ctttccggcg ggcgcatggg tgacgtcgga cgcagtcttt
    48241 tgcgtggtaa cagcgcgctg gtagatgagt gtgtcgcgtt ttatgaagaa cacttcccgg
    48301 atcgctattt tctcgagctg atccgcaccg gcaggccgga tgaagaaagc tatctgcacg
    48361 cggcggtgga actggcggaa gcgcgcggtt tgcccgtcgt ggcgactaac gatgtgcgct
    48421 ttatcgacag cagcgacttt gacgcacacg aaatccgcgt cgcgatccac gacggcttta
    48481 ccctcgacga tcctaaacgc ccgcgtaact attcgccgca gcaatatatg cgtagcgaag
    48541 aggagatgtg tgagctgttt gccgacatcc ccgaagccct tgccaacacc gttgagatcg
    48601 ccaaacgctg taacgtaact gtgcgtcttg gtgaatactt cctgccgcag ttcccgaccg
    48661 gggacatgag caccgaagat tatctggtca agcgtgcaaa agagggcctg gaagagcgtc
    48721 tggccttttt attccctgac gaggaagaac gtgttaagcg ccgcccggaa tatgacgaac
    48781 gtctggagac tgaacttcag gttatcaacc agatgggctt cccgggctac ttcctcatcg
    48841 ttatggaatt tatccagtgg tcgaaagata acggcgtacc ggtagggcca ggccgtggct
    48901 ccggtgcggg ttcactggtg gcctacgcgc tgaaaatcac cgacctcgat ccgctggaat
    48961 ttgacctgct gttcgaacgt ttccttaacc cggaacgtgt ctccatgcct gacttcgacg
    49021 ttgacttctg tatggagaaa cgcgatcagg ttatcgagca cgtagcggac atgtacggtc
    49081 gtgatgcggt atcgcagatc atcaccttcg gtacaatggc ggcgaaagcg gtgatccgcg
    49141 acgtaggccg cgtgctgggg catccgtacg gctttgtcga tcgtatctcg aaactgatcc
    49201 cgcccgatcc ggggatgacg ctggcgaaag cgtttgaagc cgagccgcag ctgccggaaa
    49261 tctacgaagc ggatgaagaa gttaaggcgc tgatcgacat ggcgcgcaaa ctggaagggg
    49321 tcacccgtaa cgccggtaag cacgccggtg gggtggttat cgcgccgacc aaaattaccg
    49381 attttgcgcc gctttactgc gatgaagagg gcaaacatcc ggtcacccag tttgataaaa
    49441 gcgacgttga atacgccgga ctggtgaagt tcgacttcct tggtttgcgt acgctcacca
    49501 tcatcaactg ggcgctggag atgatcaaca agcggcgggc gaagaatggc gagccgccgc
    49561 tggatatcgc cgcgatcccg ctggatgaca agaaaagctt cgacatgctg caacgctcgg
    49621 aaaccacggc ggtattccag cttgaatcgc gcggcatgaa ggatctgatc aagcgtctgc
    49681 aacctgactg cttcgaagat atgatcgcac tggtggcgct gttccgccca ggtccgttgc
    49741 aatcagggat ggtggataac tttatcgacc gtaaacatgg tcgcgaagaa atctcctatc
    49801 cggacgtaca gtggcagcat gaaagcctga aaccggtact ggagccaacc tacggcatca
    49861 tcctgtatca ggaacaggtc atgcagattg cgcaggtgct ttctggttat accctcggtg
    49921 gcgcggatat gctgcgtcgt gcgatgggta agaaaaagcc ggaagagatg gctaagcagc
    49981 gttctgtatt tgctgaaggt gcagaaaaga acggagtcaa cgccgaactg gcgatgaaaa
    50041 tcttcgacct ggtggagaaa ttcgcgggtt acggatttaa caaatcgcac tctgcggcct
    50101 atgctttggt gtcatatcaa acgttatggc tgaaagcgca ctatccggcg gagtttatgg
    50161 cggcggtaat gaccgccgat atggacaaca ccgagaaggt ggtgggcctg gtggatgagt
    50221 gctggcggat ggggctgaaa atcctgccac cagatataaa ctccggtctt taccatttcc
    50281 acgtcaacga cgacggcgaa atcgtgtatg gtattggcgc catcaaaggg gtaggtgaag
    50341 gtccgattga ggccatcatc gaagcccgta ataaaggcgg ctacttccgc gaactgtttg
    50401 atctctgcgc ccgtaccgac accaaaaagt taaaccggcg ggtgctggaa aaactgatca
    50461 tgtccggggc gtttgaccgt cttgggccac accgcgcggc gctgatgaac tcgctgggtg
    50521 atgcgttaaa agcggcagat caacacgcga aagcggaagc tatcggtcag gccgatatgt
    50581 tcggcgtgct ggccgaagag ccggaacaaa ttgaacaatc ctacgccagc tgccaaccgt
    50641 ggccggagca ggtggtgtta gatggggaac gtgaaacgtt aggtctgtac cttacgggac
    50701 accctatcaa ccagtattta aaagagattg agcgttatgt cggaggcgta aggctgaaag
    50761 acatgcaccc gacagaacgt ggtaaagtca tcacggctgc ggggctcgtt gttgccgcgc
    50821 gggttatggt caccaagcgc ggcaatcgta tcggtatctg cacgctggat gaccgttccg
    50881 ggcggctgga agtgatgttg tttactgacg ccctggataa ataccagcaa ttgctggaaa
    50941 aagaccgcat acttatcgtc agcggacagg tcagctttga tgacttcagc ggtgggctta
    51001 aaatgaccgc tcgcgaagtg atggatattg acgaagcccg ggaaaaatat gctagcgggc
    51061 ttgctatctc gctgacggac aggcaaattg atgaccagct tttaaaccga ctccgtcagt
    51121 ctctggaacc ccaccgctct gggacaattc cagtacatct ctactatcag agggcggatg
    51181 cacgcgcgcg gttgcgtttt ggcgcgacgt ggcgtgtctc tccgagcgat cgtttattaa
    51241 acgatctccg tggcctcatt ggttcggagc aggtggaact ggagtttgac taatacagga
    51301 atactatgag tctgaatttc cttgattttg aacagccgat tgcagagctg gaagcgaaaa
    51361 tcgattctct gactgcggtt agccgtcagg atgagaaact ggatattaac atcgatgaag
    51421 aagtgcatcg tctgcgtgaa aaaagcgtag aactgacacg taaaatcttc gccgatctcg
    51481 gtgcatggca gattgcgcaa ctggcacgcc atccacagcg tccttatacc ctggattacg
    51541 ttcgcctggc atttgatgaa tttgacgaac tggctggcga ccgcgcgtat gcagacgata
    51601 aagctatcgt cggtggtatc gcccgtcttg acggtcgtcc ggtgatgatc attggtcatc
    51661 aaaaaggtcg tgaaaccaaa gaaaaaattc gccgtaactt tggtatgcca gcgccagaag
    51721 gttaccgcaa agcactgcgt ctgatgcaaa tggctgaacg ctttaagatg cctatcatca
    51781 cctttatcga caccccgggg gcttatcctg gcgtgggcgc agaagagcgt ggtcagtctg
    51841 aagccattgc acgcaacctg cgtgaaatgt ctcgcctcgg cgtaccggta gtttgtacgg
    51901 ttatcggtga aggtggttct ggcggtgcgc tggcgattgg cgtgggcgat aaagtgaata
    51961 tgctgcaata cagctcctat tccgttatct cgccggaagg ttgtgcgtcc attctgtgga
    52021 agagcgccga caaagcgccg ctggcggctg aagcgatggg tatcatcgct ccgcgtctga
    52081 aagagctgaa actgatcgac tccatcatcc cggaaccgct gggcggcgct caccgtaacc
    52141 cggaagcgat ggcggcatcg ttgaaagcgc aactgctggc ggatctggcc gatctcgatg
    52201 tgttaagcac tgaagattta aaaaatcgtc gttatcagcg cctgatgagc tacggttacg
    52261 cgtaattcgc aaaagttctg aaaaagggtc acttcggtgg ccctttttta tcgccacggt
    52321 ttgagcaggc tatgattaag gaaggatttt ccaggaggaa cacatgaaca tcattgccat
    52381 tatgggaccg catggcgtct tttataaaga tgagcccatc aaagaactgg agtcggcgct
    52441 ggtggcgcaa ggctttcaga ttatctggcc acaaaacagt gttgatttgc tgaaatttat
    52501 cgaacataac cctcgaattt gcggcgtgat ttttgactgg gatgagtaca gtctcgattt
    52561 atgcagcgat atcaatcaac ttaatgaata tctcccgctt tatgccttca tcaacaccca
    52621 ctcgacgatg gatgtcagcg tgcaggatat gcggatggcg ctctggtttt ttgaatatgc
    52681 gctggggcag gcggaagata tcgccattcg tatgcgtcag tacaccaacg aatatcttga
    52741 taacattacg ccgccgttca cgaaagcctt gtttacctac gttaaagagc ggaagtacac
    52801 cttttgtacg ccggggcata tgggcggcac cgcatatcaa aaaagcccgg ttggctgcct
    52861 gttttatgat tttttcggcg ggaataccct caaggctgat gtctctattt cggtcaccga
    52921 gcttggttcg ttgctcgacc acaccgggcc acacctggaa gcggaagagt acatcgcgcg
    52981 gacttttggc gcggaacaga gttatatcgt taccaacgga acatcgacgt cgaacaaaat
    53041 tgtgggtatg tacgccgcgc catccggcag tacgctgttg atcgaccgca attgtcataa
    53101 atcgctggcg catctgttga tgatgaacga tgtagtgcca gtctggctga aaccgacgcg
    53161 taatgcgttg gggattcttg gtgggatccc gcaccgtgaa tttactcgcg acagcatcga
    53221 agagaaagtc gctgccacca cgcaggcaca atggccggtt catgcggtga ttaccaactc
    53281 cacctatgat ggcttgctct acaacaccga ctggatcaaa cagacgctgg atgtcccgtc
    53341 gatccacttc gattccgcct gggtgccgta cacccatttt catccgatct accaggggaa
    53401 aagtgatctt cgaaacgcaa tcgacccaca aaatgctggc ggcgttatcg caggcttcgc
    53461 tgatccacat taaaggcgag tatgacgaag aggcgtttaa cgaagccttt atgatgcata
    53521 ccaccacctc gcccagttat cccattgttg cttcggttga gacggcggcg gcgatgctgc
    53581 gtggtaatcc gggcaaacgg ctgattaacc gttcggtgga gcgggcgctg cattttcgca
    53641 aagaggtcca gcggttgcgg gaagagtctg acggttggtt tttcgatatc tggcaaccgc
    53701 cgcaggtgga tgaagccgaa tgctggcccg ttgcgccagg cgaacagtgg cacggcttta
    53761 acgatgcgga tgccgatcat atgtttctcg atccggttaa agtcactatt ttgataccgg
    53821 ggatggacga acagggcaat atgagcgagg aggggatccc ggcggcgctg gtggcaaaat
    53881 tcctcgacga acgtgggatc gtagtagaga aaaccggccc ttataacctg ctgtttctct
    53941 ttagtattgg tatcgataaa accaaagcaa tgggattatt gcgtgggttg acggaattta
    54001 aacgctctta cgatctcaac ctgcggatca aaaatatgct acccgatctc tatgctgaag
    54061 atcccgattt ctaccgcaat atgcgtattc aggatctggc gcaagggatc cataagctga
    54121 ttcgtaaaca cgatcttccc ggtttgatgt tgtgggcatt cgatactttg ccggaaatta
    54181 tcatgacgcc acatcaggca tggcagagac agattaaagg tgaagtagaa accattgcgc
    54241 tggaacaact ggtcggtaga gtatcggcaa atatgatcct gccttatcca ccgggcgtac
    54301 cgctgttgat gccaggagaa atgctgacca aagagagccg cacggtactc gattttctac
    54361 tgatgctttg ttccgtaggg caacattacc ccggttttga aacggatatt cacggcgcga
    54421 aacaggacga agacggcgtt taccgcgtac gagtcctaaa aatggcggga taacttgcca
    54481 gagcggctta cgggcgagta acgtgctgtt aacaaataaa ggagacgtta tgctgggttt
    54541 aaaacaggtt caccatattg cgattattgc gacggattat gcggtgagca aagctttcta
    54601 ctgcgatatt cttggtttca cgctgcaaag cgaagtctat cgcgaagcgc gcgactcgtg
    54661 gaaaggggat ttggcgctta atgggcaata tgtgattgag cttttctctt ttccgttccc
    54721 gccggagcgc cccagccgac cggaagcttg cggcctgcgt catctggctt ttagcgttga
    54781 tgacatcgat gcggcagtgg cgcaccttga aagccataac gtgaagtgtg aagccatccg
    54841 tgtcgatcca tacacgcaaa aacgcttcac cttctttaac gatccggacg ggctgccgtt
    54901 ggaactgtat gagcagtaag gcttgtcatc gccgcatttg cccggtaacg tgccgggcat
    54961 tgctactgta aaatcgcacc atcatgacac tcacgctcaa tagacaactt ctcacctcac
    55021 gccagatttt ggtcgccttt agcggcgggc ttgactccac cgttctgctg catcagttgg
    55081 tgcagtggcg gacggaaaat ccgggcgtca ctctgcgcgc tattcatgtg catcacggtt
    55141 taagtgccaa tgccgatgcc tgggttacgc attgcgaaaa cgtctgccaa cagtggcagg
    55201 tgccgctggt ggtcgaacgc gtacaacttg cgcaagaggg gctggggatt gaggcccagg
    55261 cgcggcaggc gcgttatcag gcatttgccc gtaccctatt gcccggcgaa gtgctggtca
    55321 ccgcgcaaca tctcgacgat caatgtgaaa cctttctgct ggcgctaaaa cgcggtagcg
    55381 gccctgccgg gctttcggct atggcggaag tttcggagtt tgccggaaca cggcttatcc
    55441 gcccgttgct tgcccgcacg cggggggaac tggcgcagtg ggcattggcg catggtttac
    55501 gctggattga agacgaaagt aatcaggacg actcatacga tcgtaacttt ctgcgcctgc
    55561 gcgtagtgcc gttattgcag cagcgttggc cgcattttgc ggaagcaacg gcccgcagcg
    55621 ccgcactttg tgctgaacaa gagagtctgt tggatgaact gctggcggat gatttagcac
    55681 actgccaaac gtcgcagggg acgctgcaga ttgcgccaat gctggcgatg agtgatgccc
    55741 gccgcgcggc gattatccgt cgctggctgg cagggcagaa tgcaccaatg ccttcccgcg
    55801 acgcgctggt gcggatctgg caggaagtgg cgctggcgcg ggaagatgcc tccccctgtt
    55861 tacgcttggg cgcgtttgaa atccgccgct atcagtcgca actgtggtgg attaaatccg
    55921 tcaccgggaa aagtgaaacc attgtgccgt ggcagacgtg gctacaaccg ctggaattac
    55981 cggcggggct gggaagcgta cagcttactg cgggaggcga tattcgccct ccgcgtgcag
    56041 acgaagcggt cagcgtgcgt ttcaaatcgg caggattgct gcatattgtc gggcgcaacg
    56101 gcggacgtaa gctgaagaaa atctggcaag agttaggcgt gccgccgtgg ctacgtgaca
    56161 ccacgccact gctgttttat ggcgaaacgc tgattgcggc ggcaggggta tttgtgacgc
    56221 aagaaggtgt ggctgaaggt gagaatggcg taagttttgt ctggcagaaa acgcttagtt
    56281 aagtgaaagc cggataagac gcatcaaacg tcgcatccgg cgaaagtcaa tcaggactcg
    56341 ctcaccacca ccgtaccgat ttccgggtgg ctaaagctgg taattttatc cagacgtagc
    56401 tcacgggttt cgccagcggc ctcgacgacc agatactcca catttttgcg ggagactaaa
    56461 tcgctggctt ttgcctgcaa tttttcgcca tctttcagct caagtgtcag cattaaatga
    56521 tgctggcagg cgagctcaag attatcgtaa tcatcacaat tgattggttg atacgtatca
    56581 ttcattaaca taatcgctca ccagtaagtt tgccgcagcg tatgctgctt tttccctgac
    56641 agcctcagaa agggcgtcgt cggcagccat ttcattcagc actttcaaaa cgcagcccag
    56701 cgcgtccgga acgtatccta agtctccgct ggcgatttcc gcgtaccgct tgcgtattaa
    56761 ctcacaatat ttttccacat gccctcctgt cagcacactg acttaaccgt ggatgcaagt
    56821 ctaagcctac gaagataaac tctgtttcgc aaggtgacta taccacactc atttctgcaa
    56881 tatcagcgcc gcaactgcac gtattccgtt acaatggcct cctgattcga aaggagtttt
    56941 cttatggcgc ttaaagcgac aatttataaa gcgacggtta atgtggccga tctcgaccgc
    57001 aaccagtttc tcgatgcctc tctgacgctg gcgcgccacc cttcagaaac ccaggagcgt
    57061 atgatgctgc gcctgctggc gtggctgaaa tatgctgatg aacgtctgca atttacccgt
    57121 ggtttgtgtg ccgatgatga gccggaagcg tggctgcgta acgatcacct gggcattgat
    57181 ttgtggattg agctggggct gccggatgag cggcggatta agaaagcctg cacccaggcc
    57241 gcagaagtgg cgctgtttac ctataatagt cgggcggcgc aaatctggtg gcagcaaaat
    57301 cagagcaaat gtgtgcagtt tgccaatctt tccgtctggt atctggacga tgaacaactg
    57361 gcgaaagtaa gcgcctttgc cgatcgtacc atgacgctgc aggcaacgat tcaggatggc
    57421 gtgatctggt tatcggacga taagaataat ctggaagtta acttaaccgc ctggcaacaa
    57481 ccttcatgat tgtgatttcc cgacatgttg ctattcccga tggtgagctt gagatcaccg
    57541 ccattcgtgc gcagggcgcg ggcgggcagc atgttaataa gacctcaacg gccattcatc
    57601 tgcgttttga cattcgggcg tccagcctgc cagagtatta caaagagcgt ctgctcgccg
    57661 ccagccatca tttgatcagc agtgatggcg tgattgtcat taaggcacag gaataccgca
    57721 gtcaggaact gaaccgcgaa gccgcgctag cccggctggt ggcagtgatt aaagatttaa
    57781 caacagaaca aaaagcccga cgacccacgc ggcccacccg tgcatcgaaa gagcgcaggc
    57841 tggcatcgaa agcacaaaaa tcaagcgcga aggcgatgcg cggcaaagta cgcagcggtc
    57901 gggaataaaa agaaggaata gatggtgaaa aaagcgatag tgacagcgat ggctgtaatc
    57961 agcctcttta ctctgatggg atgtaataac cgggccgaag tcgatacgct ttctccagca
    58021 caggctgccg aactgaaacc gatgccgcaa agttggcgcg gcgtgctgcc gtgtgctgat
    58081 tgtgaaggaa tcgaaacctc tctgttcctc gaaaaagacg gaacatgggt gatgaatgag
    58141 cgttatctcg gtgctcgtga agaaccttcc tccttcgctt cctacggtac atgggcgcga
    58201 accgctgaca agctggtatt aaccgacagc aaaggtgaaa agtcatatta tcgggcgaaa
    58261 ggcgatgcgc tggagatgct cgatcgtgaa ggcaatccga ttgaatcgca gttcaactat
    58321 acgctggaac cggcacaatc cagtttacct atgacgccga tgaccctgcg aggcatgtat
    58381 ttttatatgg ctgatgcggc gaccttcact gattgcgcga ccggaaaacg tttcatggta
    58441 gcgaataacg cagagctgga gcgtggctac ctggctgcgc gcggtcacag tgaaaaaccg
    58501 gtgttactgt cagtagaagg tcactttacg cttgaggcta atccggatac tggtgcgcca
    58561 accaaagtat tagcgcccga tacggcgggt aaattttacc cgaataagga ttgcagtagt
    58621 ttggggcagt aaacctgctt aaggcagaaa caaacgcaaa acaggccaga aggatatatt
    58681 tcaacatttt gaatttgcac gttttttgta ggccggataa ggcgtttacg ccgcatccgg
    58741 caatggtgct caacgcctga tgcgacgctg gcgcgtctta tcatgcctac aacccccctc
    58801 ataccttaag gcggataagg catttacttt atcaccgggt caaaccaaca aagcgacccg
    58861 ccttgatata aatccctggc ttcaggtgcc caacatactg taactgcgtt tctggcttaa
    58921 acgcagagac atcaccaata cgcatatgca tcagatcggc agggctaatc cagcccgatt
    58981 gtgccagtgt aagtggatgt ccggctttgg caaatgcatc agtgacaaat tccgaacaaa
    59041 accacgactt tttgtctcct tcgcccacac tgcttaactg cgctttcgcc aggccgctga
    59101 cgcactgctg gcgaaaatcc tcggagaact gattcagtga gcacatctgg cgagtcacca
    59161 taaagggaat aaattcgaca atgccgcgat agttataacc gctatctttg attttattgg
    59221 caaacgcggt gatatctgtg gcttgttgcg gggtaagatc cgggactcgt aagacgaaaa
    59281 gcttatcact atgcttcatc gcttttttaa gggaaacaat ctggacgcca gcgcctgtcg
    59341 cttctgcaac gttattatca cccagaaaga ttgcaacgtg actcacagag gaagtgctga
    59401 agacgcggat tccaaatgag gttaccccaa ggcttgagga gaacagcaaa tcgccgggtt
    59461 tgagatcagg tgccgttatt tcttttattg attgttcggt g
//
LOCUS       DADLFA010000005        51580 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000005 DADLFA010000000
VERSION     DADLFA010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 51580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51580
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.005"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(20..1192)
                     /gene="cgtA"
                     /locus_tag="JQH65_000256"
     CDS             complement(20..1192)
                     /gene="cgtA"
                     /locus_tag="JQH65_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HAY6137778.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(1208..2173)
                     /locus_tag="JQH65_000257"
     CDS             complement(1208..2173)
                     /locus_tag="JQH65_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY6137779.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(2300..2557)
                     /gene="rpmA"
                     /locus_tag="JQH65_000258"
     CDS             complement(2300..2557)
                     /gene="rpmA"
                     /locus_tag="JQH65_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAY6137780.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(2578..2889)
                     /gene="rplU"
                     /locus_tag="JQH65_000259"
     CDS             complement(2578..2889)
                     /gene="rplU"
                     /locus_tag="JQH65_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAY6137781.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            3148..4119
                     /gene="ispB"
                     /locus_tag="JQH65_000260"
     CDS             3148..4119
                     /gene="ispB"
                     /locus_tag="JQH65_000260"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HAY6137782.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            4347..4625
                     /gene="sfsB"
                     /locus_tag="JQH65_000261"
     CDS             4347..4625
                     /gene="sfsB"
                     /locus_tag="JQH65_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HAY6137783.1"
                     /translation="MESNFIDWHPPDIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(4673..5932)
                     /gene="murA"
                     /locus_tag="JQH65_000262"
     CDS             complement(4673..5932)
                     /gene="murA"
                     /locus_tag="JQH65_000262"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAY6137784.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIKRVKGE"
     gene            complement(5987..6241)
                     /gene="ibaG"
                     /locus_tag="JQH65_000263"
     CDS             complement(5987..6241)
                     /gene="ibaG"
                     /locus_tag="JQH65_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015585657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HAY6137785.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(6401..6694)
                     /gene="mlaB"
                     /locus_tag="JQH65_000264"
     CDS             complement(6401..6694)
                     /gene="mlaB"
                     /locus_tag="JQH65_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HAY6137786.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLQLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(6694..7329)
                     /gene="mlaC"
                     /locus_tag="JQH65_000265"
     CDS             complement(6694..7329)
                     /gene="mlaC"
                     /locus_tag="JQH65_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HAY6137787.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(7348..7899)
                     /gene="mlaD"
                     /locus_tag="JQH65_000266"
     CDS             complement(7348..7899)
                     /gene="mlaD"
                     /locus_tag="JQH65_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HAY6137788.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(7904..8686)
                     /gene="mlaE"
                     /locus_tag="JQH65_000267"
     CDS             complement(7904..8686)
                     /gene="mlaE"
                     /locus_tag="JQH65_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HAY6137789.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(8694..9503)
                     /gene="mlaF"
                     /locus_tag="JQH65_000268"
     CDS             complement(8694..9503)
                     /gene="mlaF"
                     /locus_tag="JQH65_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HAY6137790.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            9713..10690
                     /locus_tag="JQH65_000269"
     CDS             9713..10690
                     /locus_tag="JQH65_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HAY6137791.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            10704..11690
                     /gene="kdsD"
                     /locus_tag="JQH65_000270"
     CDS             10704..11690
                     /gene="kdsD"
                     /locus_tag="JQH65_000270"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HAY6137792.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            11711..12277
                     /gene="kdsC"
                     /locus_tag="JQH65_000271"
     CDS             11711..12277
                     /gene="kdsC"
                     /locus_tag="JQH65_000271"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HAY6137793.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSELLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            12274..12849
                     /gene="lptC"
                     /locus_tag="JQH65_000272"
     CDS             12274..12849
                     /gene="lptC"
                     /locus_tag="JQH65_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HAY6137794.1"
                     /translation="MSKARRWVTIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            12818..13375
                     /gene="lptA"
                     /locus_tag="JQH65_000273"
     CDS             12818..13375
                     /gene="lptA"
                     /locus_tag="JQH65_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HAY6137795.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            13382..14107
                     /gene="lptB"
                     /locus_tag="JQH65_000274"
     CDS             13382..14107
                     /gene="lptB"
                     /locus_tag="JQH65_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HAY6137796.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            14155..15588
                     /gene="rpoN"
                     /locus_tag="JQH65_000275"
     CDS             14155..15588
                     /gene="rpoN"
                     /locus_tag="JQH65_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAY6137797.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTSETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            15611..15898
                     /gene="hpf"
                     /locus_tag="JQH65_000276"
     CDS             15611..15898
                     /gene="hpf"
                     /locus_tag="JQH65_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HAY6137798.1"
                     /translation="MQLNITGNNVEITEALREFVRAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            16016..16507
                     /gene="ptsN"
                     /locus_tag="JQH65_000277"
     CDS             16016..16507
                     /gene="ptsN"
                     /locus_tag="JQH65_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HAY6137799.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRSVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            16553..17407
                     /gene="rapZ"
                     /locus_tag="JQH65_000278"
     CDS             16553..17407
                     /gene="rapZ"
                     /locus_tag="JQH65_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAY6137800.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            17404..17676
                     /gene="npr"
                     /locus_tag="JQH65_000279"
     CDS             17404..17676
                     /gene="npr"
                     /locus_tag="JQH65_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HAY6137801.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            17890..18522
                     /gene="yrbL"
                     /locus_tag="JQH65_000280"
     CDS             17890..18522
                     /gene="yrbL"
                     /locus_tag="JQH65_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HAY6137802.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAYLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHHISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(18519..19247)
                     /gene="mtgA"
                     /locus_tag="JQH65_000281"
     CDS             complement(18519..19247)
                     /gene="mtgA"
                     /locus_tag="JQH65_000281"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HAY6137803.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHRGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(19244..19897)
                     /gene="elbB"
                     /locus_tag="JQH65_000282"
     CDS             complement(19244..19897)
                     /gene="elbB"
                     /locus_tag="JQH65_000282"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HAY6137804.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(20127..22463)
                     /gene="arcB"
                     /locus_tag="JQH65_000283"
     CDS             complement(20127..22463)
                     /gene="arcB"
                     /locus_tag="JQH65_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HAY6137805.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(22559..23488)
                     /locus_tag="JQH65_000284"
     CDS             complement(22559..23488)
                     /locus_tag="JQH65_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAY6137806.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            24163..28623
                     /gene="gltB"
                     /locus_tag="JQH65_000285"
     CDS             24163..28623
                     /gene="gltB"
                     /locus_tag="JQH65_000285"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HAY6137807.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLNVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            28636..30054
                     /locus_tag="JQH65_000286"
     CDS             28636..30054
                     /locus_tag="JQH65_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY6137808.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEQFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENKDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            30238..30717
                     /locus_tag="JQH65_000287"
     CDS             30238..30717
                     /locus_tag="JQH65_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HAY6137809.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
     gene            30734..31039
                     /locus_tag="JQH65_000288"
     CDS             30734..31039
                     /locus_tag="JQH65_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY6137810.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(31115..31579)
                     /locus_tag="JQH65_000289"
     CDS             complement(31115..31579)
                     /locus_tag="JQH65_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF386 domain-containing protein"
                     /protein_id="HAY6137811.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIDNEQAIILKPGMFAVFMPSEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(31576..32451)
                     /locus_tag="JQH65_000290"
     CDS             complement(31576..32451)
                     /locus_tag="JQH65_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HAY6137812.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPQALRDA
                     LAALVAPLQAHAQQVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLSNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELMGADARTIFTRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(32448..33137)
                     /locus_tag="JQH65_000291"
     CDS             complement(32448..33137)
                     /locus_tag="JQH65_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAY6137813.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(33185..34675)
                     /gene="nanT"
                     /locus_tag="JQH65_000292"
     CDS             complement(33185..34675)
                     /gene="nanT"
                     /locus_tag="JQH65_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HAY6137814.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVSGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSTKNDLVKTKS"
     gene            complement(34784..35677)
                     /gene="nanA"
                     /locus_tag="JQH65_000293"
     CDS             complement(34784..35677)
                     /gene="nanA"
                     /locus_tag="JQH65_000293"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HAY6137815.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(35799..36590)
                     /gene="nanR"
                     /locus_tag="JQH65_000294"
     CDS             complement(35799..36590)
                     /gene="nanR"
                     /locus_tag="JQH65_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HAY6137816.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            36970..38336
                     /gene="dcuC"
                     /locus_tag="JQH65_000295"
                     /pseudo
     CDS             36970..38336
                     /gene="dcuC"
                     /locus_tag="JQH65_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
     gene            complement(38379..38876)
                     /gene="sspB"
                     /locus_tag="JQH65_000296"
     CDS             complement(38379..38876)
                     /gene="sspB"
                     /locus_tag="JQH65_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HAY6137817.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(38882..39520)
                     /gene="sspA"
                     /locus_tag="JQH65_000297"
     CDS             complement(38882..39520)
                     /gene="sspA"
                     /locus_tag="JQH65_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HAY6137818.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(39915..40307)
                     /gene="rpsI"
                     /locus_tag="JQH65_000298"
     CDS             complement(39915..40307)
                     /gene="rpsI"
                     /locus_tag="JQH65_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAY6137819.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(40323..40751)
                     /gene="rplM"
                     /locus_tag="JQH65_000299"
     CDS             complement(40323..40751)
                     /gene="rplM"
                     /locus_tag="JQH65_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAY6137820.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKCTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(40969..42096)
                     /locus_tag="JQH65_000300"
     CDS             complement(40969..42096)
                     /locus_tag="JQH65_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HAY6137821.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGAENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            42290..42688
                     /locus_tag="JQH65_000301"
     CDS             42290..42688
                     /locus_tag="JQH65_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HAY6137822.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            42842..44209
                     /gene="degQ"
                     /locus_tag="JQH65_000302"
     CDS             42842..44209
                     /gene="degQ"
                     /locus_tag="JQH65_000302"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HAY6137823.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDCGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            44299..45366
                     /gene="degS"
                     /locus_tag="JQH65_000303"
     CDS             44299..45366
                     /gene="degS"
                     /locus_tag="JQH65_000303"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HAY6137824.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGVNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(45429..46367)
                     /gene="mdh"
                     /locus_tag="JQH65_000304"
     CDS             complement(45429..46367)
                     /gene="mdh"
                     /locus_tag="JQH65_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HAY6137825.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            46802..47272
                     /gene="argR"
                     /locus_tag="JQH65_000305"
     CDS             46802..47272
                     /gene="argR"
                     /locus_tag="JQH65_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HAY6137826.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            47638..47901
                     /gene="yhcN"
                     /locus_tag="JQH65_000306"
     CDS             47638..47901
                     /gene="yhcN"
                     /locus_tag="JQH65_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HAY6137827.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(47957..48229)
                     /locus_tag="JQH65_000307"
     CDS             complement(47957..48229)
                     /locus_tag="JQH65_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6137828.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(48321..50288)
                     /gene="aaeB"
                     /locus_tag="JQH65_000308"
     CDS             complement(48321..50288)
                     /gene="aaeB"
                     /locus_tag="JQH65_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HAY6137829.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IIADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTGLQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETVQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQQRLLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            complement(50294..51226)
                     /gene="aaeA"
                     /locus_tag="JQH65_000309"
                     /pseudo
     CDS             complement(50294..51226)
                     /gene="aaeA"
                     /locus_tag="JQH65_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
     gene            complement(51234..51437)
                     /locus_tag="JQH65_000310"
     CDS             complement(51234..51437)
                     /locus_tag="JQH65_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HAY6137830.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
BASE COUNT        12809 a        13728 c        12883 g        12160 t
ORIGIN      
        1 atggccgggg ttttgctgat taacgcttgt aaatgaactc aacgccttct tcgtcgtctt
       61 cgtcccagtc atcatcccag tcctcatcat cctcttcagc aatctcttca agctgctggc
      121 gatgataatc atcccacatg aattcgactt tctctggctg tttcgcttct tcagcctgca
      181 cgaccgggtt ttcaatgata aaggtcatca catcccagca gagatctttc acgcccagtc
      241 cactcgccgc agagatcaga taatatttat cttcccagcc cagcgcttca gcaatcgctt
      301 tcgctttctc ttcagcttct gccttatcca gcagatcgat cttattgaac actaaccaac
      361 gcggtttcgc cgccagatcc tggctgtatt tttccagctc gctgataata atacgcgcgt
      421 tttcaaccgg atcggtgccg tcaatcggat cgatatcgat gaggtgcaac aggacgcggc
      481 aacgttccag gtgcttcagg aagcggatac ccagacctgc gccttccgca gcgccttcaa
      541 tcagtcctgg aatatcggca acaacgaagc tcttttcgtt gtccattcgt accacaccca
      601 gacttggcac cagagtggta aacggataat ccgccacttt cggtttagcc gccgataccg
      661 cgcgaataaa ggtcgattta cccgcgtttg gcatccccaa cataccgacg tcagccagca
      721 gcatcagctc cagcagcagc tcacgcttat cacccggcgt accgttggtt ttctgccgtg
      781 gtgtacggtt aacggacgat ttgaaacggg tattgcccag accgtgccag ccgcccttag
      841 caaccagcag acgctgaccg tgtttagtca tatcgcccat ggtttcacca gtaccctggt
      901 cgattacacg cgtacctacc ggcactttaa tcgtcacgtc tttaccgcgc ttaccggtac
      961 aatcgcggct tgcgccgttc tgaccgcgct ctgcacggaa agatttttca aaacgataat
     1021 cgataagcgt gttcaggttt tcgtcggctt ccatccatac gtcaccacca tcaccaccgt
     1081 cgccgccatc cgggccgcct ttcggaatat acttttcgcg gcggaagctc acgcaaccat
     1141 taccgccatc acctgcaacg accagaatcg atgcttcatc aacaaacttc attttattct
     1201 ccgtaaatca ttcgcctgcg cggggttgcg ataccaccgt tgtatgctta cgtaatccgc
     1261 cccaaatacg atgaccaatg gcggaataca tcgcgcccgc aaccacgaca aacgcaccga
     1321 gataacctaa aaggtttaac atcggtctgg cgaagaaatc gggccaggcc agtgataaaa
     1381 gatctgaaaa aaacagcgta aacagtgggg tgagcgtgat gatcgcgctc acctgcgctg
     1441 cctgccagcg agccatcgct tccgccaggg cgccatatcc taccaatgta ttcagtccgc
     1501 aaaaaattaa acatgcgagc tgccagtggc taagctgcgc tatcactcct ggctttgcca
     1561 gagggaagag cgcaattgta cataaagtgt acagcaaaaa caggatctgc ggtgaggcca
     1621 gccgacgcaa taaaaccttt tgcgccacgc cataactcac ccaaaccgtc gccgcaccga
     1681 ccccaaagat gactccccag gtgtaatcag tgagctttgt aaatatctcg accagactgg
     1741 tgttaaaaaa catcaccagg ccgctcagga gcatcaatgc ccctacaacc tgagtgctgc
     1801 gcattttctc tttcaggata aatacgctgg caaccatcat gccaactggc gagagttgcc
     1861 caatcacctg cgaagcggtc ggactcaggt attgcaagga tgagctgaac aggatgaagt
     1921 tcccaaacag cccggcggtc gccactgcca acaaaatcaa ccagcgtggc ttacgaaaca
     1981 cgcgtaatgg cggcaacctc ttcttcaccg caagaatggc acccaggcca atactcgcca
     2041 tcaagaaacg gtaaaacacg attgtcggag gttccatcac ctccagcacc tgcttcattg
     2101 cgattggcaa cgccccccag caaattgctg tggtgagcgc caaaagaatg ccaatgcctg
     2161 cctgctgctt cataccgtat tccctgcaaa gcgcatttgc ggattaacgt cgcaccatgc
     2221 tcatttttac cggttatcga atgtaaaaag ccccgcaacg tgttgcgggg ctttcatccg
     2281 ttaccgggac gcgaaaaact tattcagctt cgatgctgat aaatttacgg tttttcgggc
     2341 ctttaacttc gaatttcact ttaccgtctg ctttagcaaa cagagtgtgg tcacgaccgc
     2401 aacctacgtt agcgccagcg tggaatttgg taccacgttg acgaacgatg atgctacccg
     2461 ccagaacgga ttcgccaccg aaacgcttaa cgcccaggcg tttagcttct gaatcgcgac
     2521 cgttacgtgt ggagccgcca gcctttttat gtgccatttg aaatctctcc tcaggtctta
     2581 ggcgctgatg ccagtaattt tcacatcagt gaaccactga cgatggccct gctgcttacg
     2641 atagtgttta cgacgacgaa acttaacgat tttaactttc tcgccacgac cgtgagcaac
     2701 aacttcagct ttgattacgc cgccatcaac gaaaggaacg ccgattttga cttcttcacc
     2761 gtttgcgatc atcagcactt cagcgaactc aacagtttcg ccagttgcga tgtccagctt
     2821 ttccaggcga acggtctgac cttcgcttac tcggtgttgt ttaccaccac tttggaaaac
     2881 cgcgtacata taaaactccg cttccgcgca caccttttca atgattcaga gtgcgctata
     2941 aatattcaca atagggcgcg aatattacgc aaaacgcacg cctttgacaa gtgctacagt
     3001 caatacacga agaaaaaaaa cacaacttgt acggtaacgt ttatctgtgc cattttttca
     3061 gtacaatcac cctatattcc taaccataaa ccctaagttg cctttgttca cagtaaggta
     3121 atcggggcga aaagcccggc ttttgcgatg aatttagaaa aaatcaatga gttaaccgcg
     3181 caagatatgg cgggtgttaa tgcggcaatc cttgagcagc ttaattccga cgtccaactg
     3241 atcaatcagt taggctatta catcgtcagc ggcggcggta aacgtattcg tccgatgatt
     3301 gctgtactgg ctgcacgagc tgttggctat gagggaaatg cgcatgtcac cattgctgcc
     3361 ctgatcgagt ttatccacac ggcgactctg ctacacgacg acgttgtgga tgaatcagat
     3421 atgcgcaggg gtaaagctac cgccaacgct gcatttggca atgccgccag cgtgctggta
     3481 ggcgatttta tttatacccg cgcgttccag atgatgacca gcctcggttc actcaaagtg
     3541 ctggaagtca tgtcagaagc tgtaaacgtc atcgcagaag gtgaagttct gcaactgatg
     3601 aacgttaacg atccggacat cactgaagaa aactacatgc gcgttatcta tagcaaaacc
     3661 gcgcgtctgt ttgaggctgc cgcgcagtgt tccgggattc tggctggctg tacgccggag
     3721 gaggagaaag gcctgcagga ttatgggcgc tatctcggca ctgctttcca gttgatcgac
     3781 gatttactcg attacaatgc cgatggcgaa cagttaggta aaaacgtcgg cgacgatctg
     3841 aacgaaggta aaccgacgct gccgctgctg catgcgatgc atcatggcac accagaacag
     3901 gcacagatga tccgtaccgc catcgaacag ggtaacggtc gccatcttct ggaaccggtt
     3961 ctggaagcaa tgaacgcttg tggatctctt gaatggacgc gtcagcgcgc cgaggaagaa
     4021 gcagacaaag ccatcgcagc gttacaggtg ctcccggaca ccccttggcg agaagcactc
     4081 atcggcctcg cgcacatcgc tgttcaacgc gatcgttaat cccctccctt catcccgcgc
     4141 agtgcgcggg atgattccag taaattccat aaaaacacta attcagcgct ataacacctg
     4201 aaaaaaaggg cgatgaatct ttatatattc tgaatattca cactctttac aggaactttt
     4261 tagagcaata ggccatcagg agtatagtga tgcccgacag aagaagtgtt ctgaatgaaa
     4321 gcgactaact taaggagtga ggaaagatgg aaagtaattt cattgactgg catccccctg
     4381 acatcattgc aggtttgcgc aagaaaggaa cgtcaatggc ggcggaatct cgcagaaatg
     4441 gtttgagttc ctcaacgctg gcaaacgcat tatcgcgccc atggccgaaa ggagagatga
     4501 ttattgcgaa agccctggga actgacccct gggttatctg gccatcacgc taccatgatc
     4561 cgcagaccca tgagtttatc gacagaacgc agttgatgcg tagctacact aaaccgaaaa
     4621 aatgagtggt ctggcggtag ccccgcgaac ggggctgcca gctctcagac gattactcgc
     4681 ctttcacacg cttaatattt gcacctaaag cgcgcagttt gtcttcaatg cgttcgtagc
     4741 cacgatcgat gtgataaata cgatcaacca ccgtcgtccc ttccgcaata cagccagcca
     4801 gcaccaggct tgctgatgca cgcagatcgg ttgccataac ctgtgcacca gaaagttttt
     4861 caacaccatg acaaataacg gtattgcttt cgatttcggc atgcgcgccc atacggctaa
     4921 gctccggcac atgcataaag cggttttcaa agaccgtttc ggtgatgaat ccggtccctt
     4981 ctgccaccag gttcaacagc gtgaactggg cctgcatatc ggtcgggaat gccggatgcg
     5041 gcgcggtacg tacgttaaca gccttcggac gtttgccatg catatccagg ctaatccagt
     5101 cttcgccgac ttcgatgtcc gctccagcgt cacgcagttt cgccagcacg gcgtcgagag
     5161 tatctggctg cgcgttacgg cagataattt tgccgcgaga aatcgccgcc gccaccagga
     5221 aagtaccggt ttcgatacga tccggtagca cgcgatagac accgccgcct aaacgttcca
     5281 caccttcgat gacgatacga tcggtgccct gaccgctaat tttcgcaccc agcgtaatca
     5341 ggaagttcgc ggtatcgacg atttccggtt cacgcgctgc gttttcaata atcgtggtac
     5401 cttccgccag ggttgcagca cacatgatgg tcaccgttgc gccaacgctg actttatcca
     5461 tcacgatatg tgcgcctttc agacgaccat cgacggaagc tttaacgtaa ccttcttcca
     5521 gtttgatggt cgcgcctaat tgttcgaggc cagaaatgtg tagatcaacc ggacgcgcac
     5581 cgatcgtaca accgccaggc agtgaaactt gcccctgacc aaagcgcgct accagcggcc
     5641 ccagtgccca gatagaagca cgcatggttt taaccagatc gtaaggtgcg cagaatacat
     5701 taacgtcgcg ggcatcaata tgcacagaac cattacgttc tactttcgca cccagctgac
     5761 ttagcagctt cattgatgta tcgacgtctt tcagtttcgg gacgttctgg atctctaccg
     5821 gttcttccgc cagtagagcg gcaaaaagga taggcagagc agcattttta gcgccggaaa
     5881 ttgtgacttc gccctggagc ttcgttggcc cctgaacacg aaatttatcc atttagtttg
     5941 ttctcagtta acaattcata tccgctaccg gcgaatcgcc catagctcaa aagccgttca
     6001 gtttgcgatc gcgcgcccac tccgcagggg tatacgcttt gatcgacaca gcatgaatgc
     6061 ggttatccgc aatatattcc atcagcgggc catagaccgt ctgctgtttt ttaacccgac
     6121 tcatgccgtc aaacaactca cccacggcaa taacctgaaa gtggctgcca tcgccggaaa
     6181 cgtggacttc ctggagggag agagcgttca tcaacacgct ctgaatttca ttattttcca
     6241 tgggatcttc aatcatcagt taataaacca gcgaaacatc ttagagcaaa gttgcgctgg
     6301 cataaataag caaaaagcct cgctgataaa tcagacaagg ctcgacttgc aggcaggttt
     6361 gccggacagg cggttaacgc catatccggc ctgaaaaaat ttaacgaggc agaacatcag
     6421 caggcaaatt atacaatttc gccagggtat acactttgtc gtttaccccc tgaagcgtca
     6481 cattgttgcc ctgctttttc gccagatcga taagctggag cagcagtgcc agtccccccg
     6541 tatccacgcg ggacacacgg ctaagatcga tgcaggtaat ccccttcacc gcttcctcac
     6601 gcatttccca aagcggtagc aaaacgtcct gatccagctc tccggataac gccagcgtgt
     6661 cacccgtctg catccagctc agtgactcgc tcattatttt ttctcttcca gagtgatttt
     6721 ctgttgagaa atcgatttca gttgcgcagt caggccgtcg atacctttgg tacgcagcag
     6781 cgttccccac tcgttttgtt tggtggtgat catactgacg ccttcagcaa tcatgtcgta
     6841 agcctgccag ttgcccgtct gggagttttt acgccactgg aagtccagac gcaccggcgg
     6901 gcggccattc ggatcaataa tggtaacgcg aatcggcaca atggttttat cgcccagcgg
     6961 ctgttctggc gcaatctgat aggtttgacc atgatacatc gccagcgcct gaccgtaagc
     7021 ctgcttcagg tactcacgga aagcggcaaa gtaggcttca cgttgagcag gggtcgcact
     7081 cttgtaatac tggcccagca ccagcgcacc ggcgtatttc acctgtacgt atggcagcag
     7141 ttcctgatca acaatggtac gcagataatc cgggttggcc cgaatttgcg gttgctcatt
     7201 cttcaggcga tcgaacgttt tctgcgccgc ctcgtccatc agcttataag gattggtctg
     7261 gtctgccgcg gttgccgcac tcagcggtgc aatcaccagc aaagcgacca tcattaaacg
     7321 tttaaacatg cgtcggttct cctgaaatta tttcgttgta cccacaggtt cagtggtttc
     7381 attattacct ggcgcagcag ctggcgcatc gccactattc ttattgtcat cgcctttact
     7441 accgtaaagg aactgaccaa tgagatcttc cagcaccatc gcagacttag tgtcctgaat
     7501 tgtatcgcca tccttcagga tagcagtccc cagttccggg tcttcaaaac cgacgtttaa
     7561 tgccagatat tgttccccca gtaggccgga agtacgaatg ctcagcgaac tggtatctgg
     7621 aatgtggtta taacgttgtt caatttccag cgttacgcgt ggcagatagg ttttcgggtc
     7681 cagcgtaata tccgccaccc ggcccacaac aacgccacca atactgaccg gagagcgcgc
     7741 tttcaggccg ccaatgttat cgaacgtcgc ataaagcgtg taggtcggtt cagtacgtat
     7801 ggacgtcacg ttcgccgcct tcaggcaaac aaacagagcc gccagcagtg ctgctaataa
     7861 aaagataccc acccaaattt catttttttt cgtttgcatg tactcaattc ccaaacatca
     7921 atgcggtcag cacaaaatcc agccccagaa cagccagaga cgagtggaca acggtgcgag
     7981 tggttgcccg gctaatcccg gcagacgtcg ggatggcgtc gtagccgtta aacaacgaaa
     8041 tccacgtcac cgtgatggcg aacaccacgc tcttaatcag acagttgacc agatccatac
     8101 gccagtcgac ggcattttgc attgccgacc agaagaaccc gctatcaatg cctttccaac
     8161 tgacgccgac cagagatccg ccccagatcc ccacggcgac gaaaataacc gtcaacagtg
     8221 gtaatgaaat aaccccagcc cagaaacggg gagaaataac ccgacgcagc ggatccaccg
     8281 ccatcatctc catactggag agttgctctg tagcgcgcat caggccgatt tctgcggtta
     8341 gcgccgaacc agcacgcccg gcaaacaaca acgcggcaac caccggcccc agttcacgca
     8401 gtagcgataa cgccaccagc atacccagac tggtttccgc actataagtg gtcagaacca
     8461 gataaccttg cagccccaac accattccga tgaacacgcc agaaaccaca ataatcagca
     8521 tcgacaggac gccgacatta tagagctggc gcaccagcag cggcgcatgt ttgcgaaatt
     8581 ccggtttgcc gaccagcgca ttgaataaca ttaacccggc ccgcccgaac gttctcaggg
     8641 ttttaatccc tttatgtccg agcgacgcca gcgcatttaa cagcatgagt ggcttaactc
     8701 cctggtaaaa gatcagcgtg ataatcgccg gcaggatagc ggaacggaac aggcccgtca
     8761 gctatcccgt ccagaaactg acgtacgcgc ggatcaggat tcgcctgcaa cgcctgggca
     8821 ctgccatgag cgacaatttt tttgtccgcc aggatccagg cgtgatccgc aatacttaac
     8881 acttccggca catcgtgaga aaccaccaca caagtcacgc ccagcgcgct gttcagctca
     8941 gaaatcagct tcaccagtac gcccatggtg atgggatctt gcccaacaaa aggttcatca
     9001 aacatgatga gatccggctc cagcgcaatc gcacgcgcca gcgctgcacg ccgcgccatc
     9061 ccaccggaaa gttcagaagg cattagttta gccgctccac gcagccccac ggcctccagc
     9121 ttcatcatca ccgtactatg caacaatggc gcgggaagtt gggtatgttc gcgcagtgga
     9181 taggcgacgt tgtcaaatac gttcatatca gtgaacaacg ccccggactg aaataacatg
     9241 ctcatccgtt tgcgcactgt atacaggcgc gaacgagaca tcgccggaat attctcacca
     9301 tcgaaaagga tctcaccatg atctggtgcg atttgcccgc caatcagacg gagtagcgtc
     9361 gttttaccga tgcccgatgg ccccatgatc gccgtgatct tccctcgcgg cacggtcagg
     9421 gaaatattat cgaagatgca gcgattgcca cgcgtaaaac tgacatcgcg catatcgact
     9481 aaattcgcca cagactgctc cataattcac ccttcgtctt gcgttgattt tctaagcatg
     9541 gcgctcaatt taaccttgaa cccaacatat ttacagaata ttacccgccg tggttagcga
     9601 aagctggcat ttgttttact ttttagccgc ataaagtcaa aattaagaat ccgttacggc
     9661 tttctgaaaa tcttcagcgg accggcgagt atacctgaag aaaggacgtt agatgctttt
     9721 agctacggca ctgttaattg ttggtttact tttggtcgtt tacagtgccg accgcctggt
     9781 ttttgccgcg tctattcttt gccgaacctt tggcatcccg ccgctgatca tcggcatgac
     9841 ggtggtcagt attggtacat cgttaccaga aatcatcgtc tcgcttgccg cgtctctgca
     9901 cgaacaacgc gatttagccg ttggtacagc cctcggctca aacattatca atattttgct
     9961 gatcctcggt ctggctgcgc tggttcgtcc ttttaccgtc cattccgatg ttctgcgtcg
    10021 tgaattaccc ttaatgttgt tggtcagcgt agtggccggt tccgtactct atgacggaca
    10081 acttagtcgc agcgatggta tctttctcct ctttctggct gtgctatggc tgctgttcat
    10141 tgttaaactt gcacgtcagg ctgaacgtca ggggactgac agcctgacca gagagcagct
    10201 tgcagagctg ccgcgtgacg gcggattgcc cgtcgcgttt ttatggctcg gcattgcgct
    10261 tatcatcatg ccagtggcca cgcggatggt ggttgataac gccacggtgc tggcgaatta
    10321 ctttgccatc agcgagttga cgatgggtct gacggcaatt gctatcggaa ccagcctgcc
    10381 ggaactggca accgcaatag cgggggttcg caaaggtgaa aacgacattg ctgtcggaaa
    10441 tatcattggc gcaaacattt ttaatattgt catcgtgttg ggtttacccg cgctgataac
    10501 accaggagag attgatccat tggcgtacag tcgtgactac agcgtgatgt tgctggtgag
    10561 cattattttt gcgttgctgt gctggcggcg ctccccgcaa ccgggccgtg gtgtaggggt
    10621 attattaact ggcggattta tcgtatggct ggcgatgttg tactggttat cgccaatact
    10681 cgttgaataa ctggaaacgc attatgtcgc acgtagagtt acaaccgggt tttgactttc
    10741 agcaagcagg taaagaagtc ctggcgattg aacgtgaatg cctggcggag cttgatcaat
    10801 acatcaatca gaatttcacg cttgcctgtg aaaagatgtt ctggtgtaaa gggaaagttg
    10861 tcgtcatggg gatggggaaa tcggggcaca tcgggcgcaa aatggccgca acgtttgcca
    10921 gcaccggtac accttcattt ttcgtccatc ctggtgaagc cgcgcatggt gatttaggca
    10981 tggtcacccc acaggatgtg gtgattgcta tctctaactc aggtgaatcc agcgaaatca
    11041 cggccttaat tccagtgctt aagcgtcttc acgtaccgtt aatctgcatc accggtcgcc
    11101 cggagagcag catggcgcgc gccgcagatg tgcatctgtg tgttaaagta gcgaaagaag
    11161 cctgtccgtt agggctggca ccgaccagca gcaccaccgc cacgctggtt atgggcgatg
    11221 ccctcgctgt cgcgctgtta aaagcacgcg gctttactgc tgaagatttt gcgctctcac
    11281 acccaggcgg cgcactgggt cgtaaacttc tgctgcgcgt aaacgatatt atgcatacgg
    11341 gcgatgagat cccgcatgtt aagaaaacgg ccagtctgcg tgacgcattg ctggaagtta
    11401 cccgcaaaaa tcttggtatg actgtcattt gcgatgacaa tatgatgatt gaaggcatct
    11461 ttaccgacgg tgatttacgc cgtgtcttcg atatgggagt ggatgttcgt cagttaagta
    11521 ttgccgatgt gatgacgccg gggggaatac gcgtgcgccc tggcattctg gccgttgagg
    11581 cactgaactt aatgcagtcc cgccatatca cctccgtgat ggttgccgat ggcgaccatt
    11641 tactcggtgt gttacatatg catgatttac tgcgtgcagg cgtagtgtaa agattcaagg
    11701 ataaacaaca atgagcaaag caggtgcgtc gcttgcgacc tgttacggcc ctgtcagcgc
    11761 cgacgttata gcaaaagcag agaacattcg tctgctgatc ctcgacgttg atggcgtact
    11821 gtcagatggc ctgatttata tgggcaataa cggcgaagag ctgaaagcgt tcaatgttcg
    11881 tgacggttat ggcattcgtt gtgcgctcac ctctgatatt gaagtcgcta tcattaccgg
    11941 gcgaaaggct aaactggtag aagatcgttg tgccacattg gggatcactc acttgtatca
    12001 ggggcagtca aacaaactga tcgcctttag cgaactgctg gaaaaactgg cgattgcccc
    12061 ggaaaatgtg gcttatgtcg gcgatgatct catcgactgg ccggtaatgg aaaaagtggg
    12121 tttaagcgtc gccgtggccg atgctcatcc actgttgatc ccgcgcgccg attatgtgac
    12181 gcgcattgct ggcggtcgtg gcgcagtgcg agaagtttgc gacttattac tcctggcgca
    12241 gggcaaactg gatgaagcca aagggcaatc gatatgagta aagccagacg ttgggttacc
    12301 attgtgctat cactggcggt tctggtgatg atcggcatta atatggccga aaaagacgat
    12361 accgcccagg tggtcgtcaa caacaatgat cccacctata aaagcgagca tacggacacg
    12421 ctcgtctata acccagaagg ggcactaagc tatcgattga ttgctcaaca cgttgaatat
    12481 tattccgatc aggccgtttc gtggtttacg cagccggtac ttaccacgtt tgataaggat
    12541 aaaatcccga catggtccgt aaaagcagat aaagccaagc tgaccaatga ccggatgctc
    12601 tatttatatg gacacgttga agtcaacgca cttgtgccgg actctcaact tcgcagaatc
    12661 acgacggata acgcgcagat caatctggtg acgcaggatg ttacctctga agacctcgtc
    12721 acgttatacg gaacaacatt taactccagc ggtctgaaaa tgcgcggcaa cttacgcagc
    12781 aagaacgccg agctgattga aaaggttaga acatcctatg aaattcaaaa caaacaaact
    12841 cagccttaat cttgtgcttg ccagctcact tctggccgcc agcattccgg catttgccgt
    12901 aaccggagac actgatcagc cgatccacat tgaatcggac cagcaatctc ttgatatgca
    12961 aggcaacgtg gttaccttta ccggtaatgt catcgtcacc cagggcacca tcaaaattaa
    13021 tgccgacaaa gtggtcgtta cccgtccggg cggcgaacaa ggtaaagaag tgattgacgg
    13081 ctacggtaaa ccggcaacgt tctaccagat gcaggacaac ggtaaacccg ttgaaggtca
    13141 tgcttcccag atgcactacg aactggcaaa agattttgtc gttctgacgg gtaatgctta
    13201 tctgcagcag gtcgatagca acattaaggg cgataagatc acttacctgg tgaaagagca
    13261 gaaaatgcag gctttcagcg acaaaggcaa gcgcgtaaca accgttctgg tgccgtcgca
    13321 gctgcaggac aaaaacaaca aaggccagac cccggcacag aagaagggta attaattcgt
    13381 tatggcaaca ttaactgcaa agaaccttgc aaaagcctat aaaggccgtc gcgtggtaga
    13441 agacgtcagc ctgaccgtca actccgggga aattgtcggt ctgctggggc caaacggtgc
    13501 cggtaagacc accactttct atatggttgt tggcattgtg ccgcgcgatg cgggcaacat
    13561 cattattgat gatgacgata tcagtctgct gcctctgcat gcacgcgcgc gccgcggtat
    13621 cggctatctg ccacaggaag cctccatttt ccgtcgcctc agcgtttacg ataacctgat
    13681 ggcggtactg caaattcgtg acgacttgtc tgctgaacaa cgtgaagacc gcgcgaacga
    13741 gctgatggaa gagtttcaca ttgagcacct gcgtgacagc atggggcagt cactctccgg
    13801 gggtgaacgt cgccgtgtag aaattgcccg cgcactggct gcgaatccga aatttattct
    13861 actcgacgaa ccgtttgccg gggttgaccc gatctcggtt atcgacatta aacgcatcat
    13921 tgaacacctg cgcgacagcg gcctgggcgt actgatcact gaccacaacg tgcgtgaaac
    13981 actggcggtt tgtgaacgcg cttatatcgt cagtcagggg catttgattg cccacggcac
    14041 gcctacagaa atcttacaag acgaacacgt taagcgtgta taccttgggg aagacttcag
    14101 actctgatag ggtagaagtt tgcgacgttt tagcaggaga gtacgattct gaacatgaag
    14161 caaggtttgc aactcaggct tagccaacaa ctggcgatga cgccacagct tcaacaggcg
    14221 attcgtctgt tgcagttgtc gacgctggaa cttcagcagg agctacagca ggcgctggag
    14281 agtaatccgc tgcttgagca aatcgacact catgaagaaa tcgacaccag cgaaacgcaa
    14341 gacagtgaaa cgctggacac cgccgacgcg ctcgaacaaa aagagatgcc ggaagagctg
    14401 ccgctcgatg ccagttggga caccatttac accgctggta caccatccgg caccagcggt
    14461 gactacattg acgatgagct gccggtctat cagggcgaaa cgacgcagac cttgcaggat
    14521 tacctgatgt ggcaggtcga gctgacaccg ttttccgaca ctgaccgcgc tattgctacc
    14581 tctatcgtcg atgccgttga tgacaccggt tatctgactg tcccgctgga agatattctc
    14641 gaaagtatgg gcgatgaaga gatcgacatc gacgaggttg aagccgtcct taagcggatc
    14701 caacggtttg atccggtcgg tgtagcggca aaagatctgc gtgactgcct gctaatccaa
    14761 ctctcccaat tcgataaaac cacgccgtgg ctggaagagg ccagactgat cattagcgat
    14821 catctcgatc tgttagccaa tcacgacttc cgcactttaa tgcgcgtcac gcgtctgaaa
    14881 gaagatgtgc tgaaagaagc cgtcaatctg atccagtcgc tcgatccgcg ccccgggcag
    14941 tcgatccaga ctggcgaacc tgagtatgtc attccagatg tgctggtgcg taagcataac
    15001 ggtcactgga cggtagaact caacagtgac agcattccgc gtctgcaaat caaccagcac
    15061 tacgcctcga tgtgcaataa cgcgcgcaac gatggtgaca gccagtttat ccgcagcaat
    15121 ctgcaggatg ccaaatggtt gatcaagagt ctggaaagcc gtaacgatac gctactgcgc
    15181 gtgagtcgct gtatcgttga acagcagcaa gccttctttg agcaaggtga agaatatatg
    15241 aaaccgatgg tactggccga tatcgcccag gctgtcgaaa tgcatgaatc gacgatatct
    15301 cgcgtgacca cgcaaaaata cctgcatagt ccacgaggca tttttgaact gaagtatttc
    15361 ttttccagtc acgtcaatac cgagggcggc ggcgaagctt cctccacggc gattcgtgcg
    15421 ctggtgaaga aattaatcgc ggcggaaaac ccagcgaaac cgttgagcga cagcaagtta
    15481 acctctttgc tgtcggaaca aggtatcatg gtggcacgcc gcactgttgc gaagtaccga
    15541 gagtctttat ccattccgcc gtcaaaccag cgtaaacagc tcgtttgacc caaccgataa
    15601 ggaagacact atgcagctca acattaccgg aaataacgtc gagatcaccg aggcactgcg
    15661 cgaatttgtt agagccaaat ttgccaaact tgagcaatat tttgaccgaa tcaaccaggt
    15721 ctatgttgtt ctgaaagtgg agaaagtcac ccacacctca gatgcaacac tgcatgtaaa
    15781 cggtggtgaa attcatgcca gcgcggaagg tcaggacatg tacgctgcca ttgatggctt
    15841 aattgataag ctggcacgcc agttgaccaa acataaagat aaactgaaac aacactaatt
    15901 gtccgggcaa ttagcatgtg catggcggcc tgttgtgcgg cacaacgggc catttgtacg
    15961 gttaatgctc cgagcctgtt ccactgtttg agtgggcagg ttcttaggtg aaattatgac
    16021 aaataatgat acaactctac agcttagcag tgttcttaac agggaatgta cgcgaagccg
    16081 cgttcactgt cagagcaaaa aacgcgccct ggaaatcatc agcgagctgg cggcgaaaca
    16141 acttagcctg ccgcctcagg tggtttttga agctatcctg acgcgggaaa aaatgggcag
    16201 taccggtatc ggcaatggta ttgccattcc gcatggcaaa ctggaagaag atactctgcg
    16261 ctccgttggc gttttcgttc agctcgaaac gcctatagct ttcgatgcca tcgacaacca
    16321 accggtggac ttactttttg ccctgctggt gccggcagac caaactaaaa cgcacctgca
    16381 tactctgtcg ctggtggcga aacgtctggc ggacaaaacc atctgccgcc gtttacgcgc
    16441 agcccagagc gatgaagagc tgtatcaaat cattacggat accgaaggta ctccggatga
    16501 agcgtagtta ttcggtaatg tctcttttag acgttgtgag gagaaacagt acatggtact
    16561 gatgatcgtc agcggacgtt caggttcagg taaatctgtc gccctgcgtg cgctggaaga
    16621 tatgggtttt tactgcgtgg ataaccttcc cgtagtgttg ttacccgatc tggctcgaac
    16681 cctggccgat cgcgagattt ctgccgccgt cagcattgat gttcgtaata tgccggagtc
    16741 accagaaata ttcgaacagg cgatgagtaa cctgcctgac gctttctcac cgcaactcct
    16801 gttcctggat gccgaccgta ataccttaat tcgtcgttac agtgacacgc gccgactgca
    16861 tccgctttcc agcaaaaacc tgtcgctgga aagtgctatc gacaaagaaa gcgatttgct
    16921 ggagcctctg cgttcgcgag cggatctgat tgtcgatacc tcagaaatgt ccgttcacga
    16981 gctggctgaa atgctgcgta cccgtctgct gggtaaacgt gaacgcgaac tgaccatggt
    17041 ctttgagtct ttcggcttca aacacggtat ccctatcgat gcagattacg tctttgacgt
    17101 gcgcttcttg ccgaacccgc actgggatcc gaaactgcgt ccaatgacag gtcttgataa
    17161 acctgtcgcc gcgttcctcg accgccacac agaagtacac aattttatct accagacgcg
    17221 aagctatctt gagctatggt tacctatgct ggaaaccaac aaccgtagct acttgacggt
    17281 cgccattggt tgtaccggcg ggaagcaccg ttcggtgtat attgcagagc aactggcaga
    17341 ctacttccgc tcgcgcggta aaaacgtcca gtcacgccat cgtacgctgg aaaaacgtaa
    17401 accatgaccg tcaagcaaac tgttgaaatc acaaacaagc tgggcatgca tgcccggcct
    17461 gcaatgaagc tgtttgaatt aatgcagggt tttgacgctg aagtgctgtt acgtaatgac
    17521 gaaggcaccg aggccgaagc caacagcgtt attgcgctgc tgatgttgga ttctgccaaa
    17581 ggacggcaga ttgaagttga agcgaccggc ccacaggaag aggaagcact ggccgccgtt
    17641 atcgccctct ttaactctgg ttttgatgaa gattaatctt catcactttg acatacaact
    17701 cccttcaaac tccccccgac aataagaaaa tcacgtactg aaatcgttct caatcaacgt
    17761 catttgtaca ttttgtgcgc ttttcacttt cagaagaacc ttaagaaaac cttaagaggc
    17821 attgtttagg ttttgtttaa gttaatcgac catactggag atcgtcagaa aatatttcca
    17881 ggagatggca tgattcgctt atctgaacaa agtcccctgg gcaccggaag acatcggaaa
    17941 tgttatgcgc atccggaaga tgcccaacgc tgtatcaaga ttgtctacca tcgtggcgat
    18001 ggtggcgata aagagatccg ccgtgagtta aagtactacg cgtatcttgg tcgccggtta
    18061 aaagactgga gtggaatacc gcgctatcac ggtaccgttg agacggattg cggaaccggt
    18121 tatgtctacg atgtgattgc agattttgac ggcaaaccct ccattacgct gaccgaattt
    18181 gctgaacaat gccgttacga agaagacatt gcacaactgc gccagctact gaaacagctg
    18241 aagcgttatt tgcaggataa ccgtatcgtg acgatgtcgc tgaagccgca gaatatcctc
    18301 tgtcaccaca tcagtgagtc agaggttatc ccggtcgtct gcgacaacat tggtgaaagc
    18361 acattgattc cactggcgac ctggtcgaag tggtgttgtc tgcgtaagca agagcggtta
    18421 tggaaacgat ttatcgccca accggcgctg gctattgccc tgcagaaaga tctgcaacct
    18481 cgtgagagca aaacgctggc cctcacttcg cgcgaagctt aatccagctg gtgttgttgc
    18541 ataaacggct caccgcctaa ctgatacatc tgccgtaaaa tccacgcctg acggctacgc
    18601 acgtagcccg atggcgagga gactttgaaa cgaagtggat taggtaatac agctgccagt
    18661 aatgcagctt ccgaccgggt aagtttgctc gcgggtttgt ggaaataacg ttgtgccgca
    18721 gcttcgacgc caaacacacc gtcgccaaat tcggcgatat tcaggtaaac cgtcaggata
    18781 cgctttttgc tccagactgt ttctatcccc agcgttaatc cagcttccag cccttttcgt
    18841 acccagctac gcccatccca taaaaagaga tttttggccg tctgctgaga aatcgttgaa
    18901 gcaccgcgaa tacggttttc attgcgctcg ttgtgcgcca gggctttctc aatggaagcg
    18961 acatcaaagc cccggtgctc aggaaatttc tgatcttctg cggcaatcac cgccagtccc
    19021 atccacggcg agatttgatc catactgacc cagtcagaat gtgccacgta acgaaaattg
    19081 ccatgcagcc aggcgctgac ctgtcgctcg accattaccg ctgagaaggg aacaggcgca
    19141 acactaaaca acgcgatgcc cccgccccag aaaacggcga ggacaaccat taaccgcaaa
    19201 agaaaacggc gaacgaaact aaacaccgtt aagcggcttt tactcattca gccagaacca
    19261 gcacgcggga aaccagctta tcaatgccgc tcgccgcttc tgcaatgttc tgcgccagca
    19321 tatatgctgg ggtggtgaca atcttattgt cttcatcaac cacgatatca tcgacaggac
    19381 agggcacatg ctccgcgccc atctcttcca gcacttctgc ggtatcgata tcagtaccga
    19441 tggtcaaacg cagcgggaaa tcgaaaattt tcggcagcat cgccggggca atacacataa
    19501 aaccaagcgg ttttccggcc tgatgcatcg cttgtgccag cgcctttaat tcacggtcaa
    19561 cggtgcattc gctaccaaga gtggcaaaat tgcttaaatt cttcgccgcg ccaaaccccc
    19621 ccggcacaat caacgcatcc agttcagcgg catcggcctg ggccagagga cggatttcac
    19681 cgcgcgttat tcgtgccgct tcaatcagca cgttgcgcgt ttccgtcatc gcttcgccag
    19741 ttaaatggtt gataacatca acctgctgct tatcaggtgc aaagcagacc gcctgcgcac
    19801 cgctgcgtga aatagctaac aacgtcaaca ccgcttcatg aatttcagaa ccgtcataga
    19861 cgccgcatcc gctcagaatt acgccaattt tcttcattgt gatcatcctt tcgcaattga
    19921 ctgaaacaca ttaatttttt taataaaaat ggtacgcatc acacatttaa ctgattcatg
    19981 taacaaatca tttaagtttt gctatcttaa ctgcgtgcgg cctgaaaaac agtgctgtgc
    20041 ccttgtaact catcataata atttacggcg cagccaagat ttccctggtg ttggcgcagt
    20101 attcgcgcac cccggtctag ccggggtcat tttttagtgg cttttgccac ccacgctttc
    20161 agcacttcta cgtcgtgacg ccactcttct ttcatctctt caatccattc accgacgtta
    20221 tcttcccagg caggaaggtc aggagactga atttgctgac ccagctgttg cagatggcgt
    20281 aaccccactg accccgccgc acctttaatt ttatgtcctt cctcaacaat gcctttttta
    20341 tcctgcgccg tcagattcga ctccagcacg ctgacatagc ccggcatcat cttctcaaac
    20401 accgctaacc cgtcggtgat cagcttcggt cctacaagtt cgagatactg ttccagcatg
    20461 ggaatatcga gcaatgcttc tgatttactg ttctcttctg tcgtcaccgt actctcctca
    20521 tcatcctggg tatcccagaa tttcttgatc atcgcggtta gcgccggaac cgaaagcggc
    20581 ttactcagca catcatccat tccagcattg aggtactctt gtttgtcttt cagcacgtta
    20641 gcggttaagg ccaccagcgg cggtaaatcc tcgcgcggat aacgcttcgt cagttcacga
    20701 gagatatcca gcccggtcat atctggcaac tgaatatcca gcaacaccag gtcgtattcg
    20761 cccggtttaa acatctccag cgccgccttg ccggtcatgg cgacatcaac gctgttacct
    20821 aatttttcca gcacagaacg cgcaacaatc acgttcagtt caatgtcttc caccagcagc
    20881 acattcagcg ccggtaaagg catatcgtct tcatcaaacg catcatcgac ctcttctgct
    20941 accgacggtg cgtggatcgt caacgtaaag gttgaacctt tgccctgttc gctggtaacc
    21001 gtaatatcgc cgcccatatt tttcgccaga cgacgagaaa cggccagacc aataccggtg
    21061 ccggtggcag gtttaccgcc atgactgtct ttcacctggt aatacatggc gaaaatttta
    21121 tccagctcat cctgcggaat gccgatacca gagtcttcca cttcaaaatg cagcatatcg
    21181 ccttcatcgt agcgcacgcg cacggtaacc tggccttgct gggtgaattt gacggcgtta
    21241 ctgatgaggt tccacaggat ctgccgtaaa cgcgtcccgt cggtaatgac ctgatgcggt
    21301 aatggcagcg tcggctccag gttaaagcgc aatccttttt gttgcgcctg caatgcggag
    21361 agattttcca gatcggcaag gaagctggtg aaatcaaccg gttgattatc aagctggacc
    21421 ttgcgccgtt ccatcttatc catgtcgata atatcgttaa agatattccc cagcgtgacg
    21481 gccgaaacat ggatggtctt gagatatttt tcctgctcgg cggtgagttc ggtatccagc
    21541 agaatgcggc tcagaccgac gataccgttc agcggtgtac gcaattcgtg actgatggtg
    21601 gagataaacg tcgttttgtc gcggctggcc cgttcaagcg catcctgata ccgcttacgc
    21661 tcggtaatgt cgcgaccaaa gcccatcaaa ccgtgacgtt tacccacgcg gtcgtagtac
    21721 ggcactttac ggatttcaaa gcaggctttg cgcccgtccg ggtaatccag ccactgttca
    21781 taggtcagtg acacattatg acggaacact ttttcatcgg tttcaatgac ttttgcggcg
    21841 gcttccggtg agtaaacatc agcaggtttc aggtgaacca gttgtttttc gctttttccg
    21901 gtcagcagct ccatcgcgcg gttacagccg gaaaactctt tatcttcgtt acgataaaaa
    21961 accaggtcgg gtgaagcatc aaggaaggaa cgtaagaatg aggattgctg ctcgagctga
    22021 atttgtgtct cttcgcgctc tttgatttca attttcagtt ggccgaaggt ttcctgcagt
    22081 tctgcttccg ctttttcacg aacggcaatt tcctgattta gctgggcaat attatctttt
    22141 aactgaacgt tgaggctcaa atcgcgctcg cgcatctcct ccagtttttg caccagccgt
    22201 gacagacgtt gtcgtgactc ctccagttgc tcgacgacca ccgatagaaa gtagaccgcc
    22261 cacggcgtaa tcagcaaacc aaagaagata gaacgaataa catcaatgct ttcgacctga
    22321 ccatgcagca ccatggttac cgccatttgt accacaatgg caagaacgac gagggccagc
    22381 gccagcaaca ttgagaagcg caccagacct aacttcatca tcaggtcaac ataatactgc
    22441 gccagcagac gaatttgctt cattagggaa ttccttcacg acaacctgcc ccaataatac
    22501 ccaattctga cagttacgtt ataaattgtg tgagaaatgc ggaattcccc tcaacgacct
    22561 actccgttgg aggtagccac ggacgtccca gcgccgatcc ctgtacgcca tgttcattca
    22621 gatagcgatc cagctcgacc attcccgtcc atcgattttc gcaccacagc ggagcaagca
    22681 gcgtcgggcg acgggcgctg gcggaaatgc ggtggtagat cacttccggt ggcgtatggc
    22741 gaatcatttc tccggcagtg agcgtgtaat cctccagttc aataccgttc aaacgccccg
    22801 cttcccaggc tttcgccata atgctgcctt tcacaatatg cagcggatgc agctttatgc
    22861 cgtccacgcc ggtttcaacc actcgctcca gcgtttgcaa acattcagcc tgcccttcac
    22921 caggcaggcc gacaattaag tgggaacaca ccttcagccc gcgctgacgt gccagctggg
    22981 ttgtacgctg ataacaggca aaatcatggc cgcggttgat gcgatgcagt gttttgtcgt
    23041 gggcggtttg tagccccagc tccagccaca cttcgtaacc ctggtcctta tattcgcaaa
    23101 gcagatccag caccgcatcc ggcacgcagt ccgggcgggt accaacacac aaaccgacaa
    23161 tattggcctg gctcaccgcc tgctgataca tagaacgcag cacctgaact tccgcaaagg
    23221 tgctagtata cgcctgaaag taggccagat agcgtttagc gcggttaact aaattcgcct
    23281 ggtgcgccag ttgctcggca atggaacgat gctgctgcgc ttcatcggca aacgaggcaa
    23341 cattacagaa tgtgcagcca ccacgcccga tggtaccgtc acggttaggg cagctaaaac
    23401 cgccgtgcag cgtcagctta tgcacctttt gcccataacg acgggtgaga tcaccaccaa
    23461 acatattgac taatttctgt aactgcataa tctgatagac cgcgccttga aaagaggcca
    23521 aagcctgcca tttttagctc aattcggcga tgacctggat caatcgtccc ggcctgcttt
    23581 tatcaactgc ataatcaatc aaaattaccg aaatttcatg cataatcaca taaatcactt
    23641 ttgcttatct tgtgtcagat ttttttatct ccttatggat tttaggcaaa aacagtagca
    23701 tgaaacgtca ttaccaatta aggcagtata aaatgctggt tttctcgtca gttcaaggta
    23761 ggataagggt taacacgcct ttatgacagt caggaattga ctgtttctct aacgacttcc
    23821 cttttagcct taaagataaa attcatttta atttcagtta cttaataaag aattttgcgc
    23881 taaagcacat ttctgtacca ataagcttgc catttgacct gtatcagctt tcccgataag
    23941 ttggaaatcc gctggaagct ttctggatga gcagcctgct catcatattt atgcagtaat
    24001 tgagatcccc tcttcaccgt attaaccgat gcgaaaagga caacaagggg gcgaatacga
    24061 ggcgcgcgta tgacacgcaa cccccgtcgc cacgctcttt ctgtgcccgt gcgcagcggt
    24121 tcggaagtgg ggttcccgca gagcctgggg gaggttcacg atatgttgta cgataaatcc
    24181 cttgagaggg ataactgtgg tttcggcctg atcgcccaca tagaaggcga acctagccac
    24241 aaggtagtgc gtactgcaat acacgcactg gcccgcatgc agcaccgtgg cgcgattctc
    24301 gccgatggta aaaccggtga cggttgcggc ttgctgttac aaaaaccgga tcgctttttt
    24361 cgcatcgttg cgcaggagcg cggctggcgt ttagcaaaaa actacgctgt cgggatgctc
    24421 ttcctgaata aagatcctga actcgcggct gccgcacgcc gcatcgttga agaagagctg
    24481 caacgcgaaa ccttgtcgat tgtgggctgg cgtgatgtcc ccactaacga aggcgtgctg
    24541 ggtgaaatcg ccctctcctc tctgccccgc attgagcaaa ttttcgttaa cgctccggca
    24601 ggctggcgtc cgcgggatat ggagcgccgt ctgtttatcg cccgccgccg cattgaaaag
    24661 cgtctcgaag ccgacaaaga cttctacgtc tgtagcctgt cgaatctggt gaacatctat
    24721 aaaggtctgt gtatgccggc ggatctgccg cgcttttatc tggatcttgc ggacctgcgt
    24781 ctggaatcgg ccatctgcct gttccaccag cgcttctcca ctaacaccgt accgcgctgg
    24841 ccgctggcgc agccgttccg ctacctggcg cataacggtg aaatcaacac catcaccggt
    24901 aaccgccaat gggcgcgtgc gcgtacctat aaattccaga cgccgcttat ccctgatctg
    24961 cacgacgccg caccgttcgt caacgaaacc ggctctgact ccagttcgat ggataacatg
    25021 ctggaactgc tgctggcagg cgggatggat atcatccgcg ccatgcgtct gttagtgcca
    25081 cccgcctggc agaacaaccc ggatatggac ccggaactgc gtgccttctt tgactttaac
    25141 tccatgcata tggagccgtg ggatggcccg gcgggcatcg tgatgtccga cggtcgtttt
    25201 gccgcctgta acctcgaccg taacggtctg cgtccggcgc gctacgtcat caccaaagat
    25261 aagctcatca cctgcgcctc tgaagtcggt atctgggatt accagcctga cgaagtggtc
    25321 gaaaaaggcc gcgtcgggcc aggcgaactg atggttatcg acacccgcag tgggcgtatt
    25381 ctgcactcgg cagaaaccga tgacgatctg aaaagccgcc atccatataa agagtggatg
    25441 gagaaaaacg tccgccgact ggtaccgttt gaagatctgc ccgatgaaga agtgggtagc
    25501 cgcgaattgg acgacgacac gcttgccagc taccagaaac agtttaacta cagtgcggaa
    25561 gagctggact ccgtaattcg cgtactgggc gaaaacggtc aggaagcggt cggttcgatg
    25621 ggcgatgata ccccattcgc cgtgctctcc agtcagccgc gcattattta cgactacttc
    25681 cgccagcagt ttgcccaggt gactaacccg ccaatcgacc cgctgcgtga agcgcatgtt
    25741 atgtcgctcg ccaccagtat cggtcgtgaa atgaacgtct tttgcgaagc agagggccag
    25801 gcgcaccgtt taagctttaa atcgccgatt ctgctctact ccgatttcaa acagctcacg
    25861 acgatgaaag aggagcacta ccgcgcagat acgctggata tcacctttga cgtcactaaa
    25921 accacgctcg aagcgacagt caaagagctg tgcgacaaag ccgaaaaaat ggtacgtagc
    25981 ggcaccgtgc tgctggtgct ctccgaccgg aatatcgcta aagatcgcct gccggttcca
    26041 gccccgatgg cggttggcgc gatccagacc cgtctggtcg atcaaagcct gcgttgcgat
    26101 gccaacatca tcgtcgaaac cgccagcgcc cgcgatccgc accacttcgc cgtgttgctg
    26161 ggcttcggcg cgacggctat ttatccatac cttgcctatg aaacgctggg ccgcctggta
    26221 gacacccatg cgattgccaa agattatcgt accgtgatgc tcaactaccg taacggcatc
    26281 aacaaaggct tgtacaaaat catgtccaaa atgggcatct ccaccatcgc ctcttaccgc
    26341 tgctcgaaac tgtttgaagc ggtcggtctg cacgatgatg tagtgggcct gtgcttccag
    26401 ggggcggtca gccgcattgg tggggcgagc tttgaagact tccagcagga tctgctgaac
    26461 ctgtcgaaac gtgcctggct ggcgcgtaag cccatcagcc agggcggcct gctgaaatac
    26521 gtccacggcg gcgaatacca cgcttacaac ccggacgtgg tgcgcacgct gcaacaggcg
    26581 gtacaaagcg gcgagtacag cgactatcag gaatacgcga agctggttaa tgagcgtccg
    26641 gcaaccacgc tgcgcgatct gctggcaatt acgccgggtg aaaacgcggt caacattgct
    26701 gatgttgaac cggcaagcga actgtttaaa cgctttgata ccgccgcgat gtctatcggc
    26761 gcgttaagcc cggaagccca cgaggcgctg gcggaagcga tgaatagcat cggcggtaac
    26821 tcgaactccg gtgaaggcgg cgaagacccg gcgcgctatg gcactaacaa agtgtcgcgc
    26881 atcaagcagg tggcttctgg tcgcttcggc gttactccgg cgtatctggt caatgccgac
    26941 gtcattcaga ttaaagtcgc ccagggcgcg aagccgggcg aaggcggtca gttgccgggc
    27001 gacaaagtca ctccttacat cgccaaactg cgctattcgg tgcccggagt gacgctgatc
    27061 tccccgccgc cgcaccacga tatctactct atcgaggact tagcgcagct cattttcgac
    27121 ctcaagcagg ttaacccgaa agcgatgatc tccgtgaagc tggtttccga accgggcgta
    27181 ggcaccatcg cgactggcgt ggcaaaagct tatgccgatt taatcaccat cgcaggctat
    27241 gacggtggca ccggcgcaag cccgctgtcg tcagtgaaat acgcaggttg cccgtgggag
    27301 ctggggcttg ttgaaaccca gcaggcgctg gttgccaatg gtctgcgtca taagatccgt
    27361 ttgcaggtcg atggcggcct gaaaaccggt gttgatatca tcaaagcggc gattctcggc
    27421 gcagaaagct tcggcttcgg cactggccca atggtcgcgc tcggctgtaa atatctgcgt
    27481 atttgccacc tgaacaactg cgcaacgggt gtggcaactc aggatgacaa actgcgtaag
    27541 aatcactatc acggcctgcc gttcaaggtg acgaattact ttgagtttat cgcccgtgaa
    27601 acccgcgagc tgatggcaca gctgggggta acgcggctgg tggatctgat tggtcgcacc
    27661 gacctgctga aagagctgga cggtttcacc gccaaacagc agaaactggc gctgtcgaag
    27721 ctgctggaga ccgccgaacc gcatccaggt aaggcactct actgcaccga aaacaacccg
    27781 ccgtttgata acggcctgct gaacgcgcag ttgctgcaac aggcgaaacc gtttgtcgat
    27841 gaacgccaga gcaaaacctt ctggttcgat atccgcaata ccgatcgctc cgtgggcgcg
    27901 tcgctttcag gctatatcgc ccagacgcac ggcgatcagg ggctggcagc cgatcctatc
    27961 aaagcgtact tcaacggcac cgcaggccag agtttcggtg tatggaacgc gggcggcgtg
    28021 gaactgtacc tgaccgggga tgccaacgac tatgtcggta aaggcatggc gggcggctta
    28081 atcgccattc gtcctccggt tggttccgcc ttccgcagcc atgaagcaag tattatcggc
    28141 aacacctgcc tgtatggcgc gaccggtggt cgtctgtatg ccgcaggccg cgcgggtgaa
    28201 cgtttcggcg tgcggaactc cggtgctatc accgtggtag aaggcattgg cgacaacggc
    28261 tgtgaatata tgacgggcgg tattgtctgt attctgggta aaaccggcgt taacttcggt
    28321 gcgggcatga ccggcggctt cgcttacgtt ctcgatgaaa gcggtgattt ccgcaaacgc
    28381 gttaacccgg aactggtcga ggtcttaaac gttgacgatc tggcgatcca tgaagagcat
    28441 ctgcgcggtc ttatcaccga gcatgttcag catactggct ctcagcgcgg tgaagagatt
    28501 ctggcgaact ggtcaacctt cgccactaaa tttgcgctgg ttaaaccgaa gtccagtgat
    28561 gtaaaagcac tgctgggtca ccgtagtcgt agcgcagcag agttgcgcgt gcaggcgcag
    28621 taaggggtag caacaatgag tcagaatgtt tatcaattta tcgacctgca gcgcgttgat
    28681 ccgcctaaga aaccgctgaa gatccgcaaa attgagtttg ttgaaattta cgagcagttt
    28741 tccgaaggcc aggccaaagc gcaggctgac cgctgcctgt cgtgcggcaa cccatactgc
    28801 gagtggaaat gtccggtaca caactacatc ccgaactggc tgaagctcgc caacgagggg
    28861 cgtatttttg aagcggcgga actgtcgcac cagaccaaca ccctgccgga agtttgcgga
    28921 cgagtctgcc cgcaagaccg tctgtgcgaa ggttcctgca ctctgaacga tgagtttggc
    28981 gcggtgacca tcggcaacat tgagcgttat atcaatgata aagcgttcga aatgggctgg
    29041 cgtccggata tgtccggcgt gaagcaaacc ggtaaaaaag tggcgattat cggcgcaggc
    29101 ccggcaggtc tggcgtgtgc ggatgtcctg acgcgtaacg gcgtaaaagc cgttgtcttc
    29161 gaccgtcatc cagaaattgg cgggctgctg accttcggta ttccggcctt caagctggaa
    29221 aaagaggtaa tgacgcgtcg ccgtgaaatc ttcaccggca tgggtattga attcaaactc
    29281 aataccgaag tgggccgcga cgtgcagctg gacgatctgc tgagtgatta cgatgccgtc
    29341 ttccttggcg tcgggactta tcagtcaatg cgcggcggtc tggaaaacaa agacgccgat
    29401 ggcgtgtacg cagcgctgcc gttccttatc gccaacacca aacagttaat gggctttggt
    29461 gaaacccgcg acgaaccgtt cgtcagcatg gaaggcaaac gcgtggtggt ccttggcggt
    29521 ggcgacactg cgatggactg cgtgcgtacg tccgtgcgcc agggagcgaa gcacgttacc
    29581 tgtgcctatc gtcgtgatga agagaacatg ccgggttccc gccgcgaagt gaaaaacgcg
    29641 cgggaagaag gcgtagagtt caaattcaac gtccagccac tggggattga agtgaacggt
    29701 aacggcaaag tcagcggcgt aaaaatggta cgtaccgaaa tgggcgaacc ggatgccaaa
    29761 ggccgtcgcc gcgcggagat cgttgcaggt tccgaacata tcgttccggc agatgcagtg
    29821 atcatggcgt ttggtttccg tccacacaac atggaatggc tggcaaaaca cagcgtcgag
    29881 ctggattcac aaggccgcat catcgccccg gaaggcagcg acaacgcctt ccagaccagc
    29941 aacccgaaaa tctttgctgg cggcgatatc gtccgtggtt ccgatctggt ggtaaccgct
    30001 attgccgaag gtcgtaaggc ggcagacggt attatgaact ggctggaagt ttaagctctg
    30061 tcccagcgcc gcctcctcag aaaagagtcg gcgctgaaat tctcctttct ttctggaaag
    30121 cgcctcgcat aatctgaaga aaaccaatgt aatacgctca tctggattgt taccggtaga
    30181 tgcccggcgt atagctgatt ccattattca acgcagcgcc atataaaggg acagataatg
    30241 gaatcgctct cagagagaac ctcaacaggc taccagcaaa tccacgacgg tattattcac
    30301 ctggtcgata gcgcccggac ggaaacggta cgtagcgtta acgcgctgat gaccgcgaca
    30361 tactgggaaa ttggccgacg aattgtcgaa tttgaacaag gtggcgaggc aagggctgcg
    30421 tatggtgcgc agctaatcaa gcgactatca aaggatttaa gtctaaggta taagcgtggg
    30481 ttctctgcaa aaaacttacg ccaaatgagg cttttttacc tcttttttca acatgttgaa
    30541 attcgccaga cagtgtctgg cgaattaaca ccattgggaa ttccgcagac accatctgca
    30601 gaattcccat cggcaaagat ttggcagacg ctgtctgcca aattgagagg ctgtttgata
    30661 tttccacagc ttttgtattg catctcctat tttcggtttg tatattcatg caaataaacg
    30721 aaggaaagtt gccatgattt acattgaaca gcttgaatta atccataaaa gtggtgatgt
    30781 gttgtatcca gtaaaaataa ccagaaaatc atcaggaaaa actgccttcc atcttgttcc
    30841 attcggctta aataagactc acgaccttct agaggtggaa gatgcttcag aagcaattcg
    30901 gctggtgatt gatgaacgtc attccattcg ctgcagtaca cttacagcaa ctattacgaa
    30961 taaaaagggc aagcgaatta agcggacagg tatatacagt atcaagggtg ttaacataaa
    31021 agaatacaac gtcaggtaat ggcacagagc ggtccgtcag ttcaggtttg gctctgtgcc
    31081 aaagatgggt caggtcacac ctgatctaat aaatttaagc cattaaatca gccttaacct
    31141 tcaccaccac ctttttaatc tcacccggct cgccgacaac gcatcctggt ttatgcggtt
    31201 cacttggcat aaacacggcg aacattcccg gttttaagat gatggcttgc tcgttatcaa
    31261 tggtgctgca aagctgataa tcatcctcat ggtggaactc ttcacactgg cgcgcagtgc
    31321 ctgccatgcc aaacagaatc cgttcctcac cgtttaataa cagctggata tcaatgtatt
    31381 gctcgtgcaa ttccgctttt ttctcgacgg gcgattgagt gttaaacgtc atgacattca
    31441 taaagatatt gtcgccctgt aattcgtaac gaccaggcgc tttttcttgc ggtctggcag
    31501 ctaatgccag cgttaacgcg tcctgtaacg caggatgtaa cccagcagac ggtaatgact
    31561 gtacttcacc catcatcata atttttctcc ctgggccaac agcgcagccc caagtaaacc
    31621 tgcatcgtgg cggtagtgcg ccgccagtaa atcaacatga aatgccgctg gctcctgcgc
    31681 cagatacgtt tccaccaacg ccagataccc ttctgccaga ccaacgctgc caccgaccac
    31741 cacgcactgg caatcagttg tggctttaat atcagcgatc agccttgcca gcacatgtgc
    31801 ggagcggtga atcagttgct gcgcctgctc gtcgccctgc ccggcgcgtg tgaaaatagt
    31861 tctcgcatcc gcgcccatga gttcaccttg cgctgccgct gcaataccgc gaccagaagc
    31921 aatcgcttcc acgcaacctg tgcgtccaca gccgcagact gggccgtgtg gatcggcaag
    31981 cgtatgcccg atgtgccccg ccagaccgcc agggccggta agcagtttac cgccgctcac
    32041 tacaccgccg ccgacgccag tggaaacggt gataaacacc atatcggtta tatcgccttc
    32101 cagcgcctga tactctgccc atgctgcggc ctgcgcgtcg ttaatggcaa tggtcggcaa
    32161 attggaaagt tgttccagcg ttttgactaa cggaaagtgt agcaatccac caagattatg
    32221 cggattcagc gccagcaagc tgccgtcgcg aataattccg gttgaagcga tggcaacctg
    32281 ctgcgcatga gcttgcaacg gggcgactaa cgcggctaag gcatcacgca acgcctgcgg
    32341 agtctggctg gctggtgtcg gaagttctcg gcgatcgcgg atctgcccgt cagcgccaat
    32401 cagcgcggcg gcaagtttag taccgccgat atcaatcgcc aacgtggtca tagcaccgcc
    32461 tttttcatcg ctgtgttgta ccactgacaa atgtgttcaa gacgcgtaat ggcagaaccg
    32521 accgtcaccg cccacgcgcc gtggcgcatc gcatctgccg cctgagcagg cgtgttgtaa
    32581 cgcccttcgg caatcacccg acatccggcg tcgctcaacg ttttcaccag cgccagatcc
    32641 ggctcttctg gcgtttcagg cgtggtatag ccagaaagcg tagtgccaat aatttcggct
    32701 cccagctttt ggcatgccag gccgtcttcc ggcgttgagc agtcggtcat cgccagtaaa
    32761 ccgtgatggt gaatacgtgc cagcagcgtt tcaacaggca ccggacgcgg gcggtcggtg
    32821 ccgtcaatgg cgataatgtc cgcgcccgcc tgcgccagcg catcaacatc ttcaatatag
    32881 gccgtgatgc gtaccggaga atcctccaga tcgcgtttca caattccaat aatcggcacg
    32941 ctcaccaccg cacgcgtggc ttgcagattt gccacacctt caatgcgaat ggcaaccgcg
    33001 cccgcctgtt ctgccgctaa tgccatggca gcgacgattt cgggtttatc gagcgggctg
    33061 tccggaaccg gctggcagga gacaatcagg ccaccgttag cagcgatttt ttgatccagt
    33121 tgtgcaagta acgacataca tcttccctta gcgaaaggcc cggtacagag accgggcaac
    33181 aggattaact tttggttttg actaaatcgt ttttggtgct gccaaatggc acggcaccgc
    33241 taaatggttt gccgtcgata gcgtcatgag tacgcaatgc ttccgggcgc agccaacgct
    33301 gaacgcggga aggcatatcc agaccaatca gcaggatcac cacaaatgtc agactgaacg
    33361 atagcgatgc cagcgcagta cccagatcca gacgttgagc gatcaacgcg ccgatgattg
    33421 gggccagtgc gccgcccaat gcgccaacgt tgtaggtaaa gcccaggccc gctgcacgct
    33481 ggtcggtatc gaaataaccg ccaatcagtt tcggtaagat cccggcgatc ccttgtccaa
    33541 gcatttgctg gaagaacagt aacagaccga gcacccagac gtttgcgccg ccaatcgcaa
    33601 ataccggaat aatcaacagc tgcgaggcca acaggctaca aacgtaggct ttgcgggttc
    33661 ccagccagtc accgaggaag ccgctgacac agcaccccac cgcagcccca aagccgctaa
    33721 agaacagcac attggcgaca gtatgcgggt tataagccag atcggttttc agatacgttg
    33781 gcagcagcgc ctgaatcggc catgagtaga ggaaagcaaa caacacgacc accatcagca
    33841 ttacgcccgt tggccagcgt ttacccgtac tttgcaccat aaagctgata aagattgcgg
    33901 cgcataacag cccaagaaca gcgacgatcg cggcattttg caggttaccg gcgaagcaaa
    33961 accacagcgc agtagccgcc gccagtgtca ttacgatatt ggcaatgcga tgttcaccac
    34021 ggtagagaat atctaccatc gtgcgtactg gtgctttacc tgcatgcttc tctttccagt
    34081 cttccgcttc cgggatgttt ttacgcagcc agagagcaaa gatgataggc aaaatgccga
    34141 taaagaacag cgcacgccag ccccagaccg gaaccaccag gctatagacc tgagcggcaa
    34201 cgacggcccc cacagagaag cctgaaatca gaaaaccact ggctttgtta cgcagatgtt
    34261 ttggccagct ttcaatgaca taggtggcgc tggaaccgta ttcacccgcc atccccatgc
    34321 cgatgaccag acgcgcgata aacatggtga tgtagcctgg cgcaaagccg caggccagcg
    34381 tcccggccga gaagagaacg atgctggtga ccattgccag acgacgcccg tagcggtcac
    34441 ccatagcgcc gagcatcagg ccgccgaacc agcgagagat aaaggctgcg gagatcagac
    34501 ttgccgcctg caccgtcgtc agcccgaatt caccctgtac ttcggtgagt accagggcga
    34561 ttaaaacgaa atcaaaaccg tcaagcagat atcccaacca ggcagcggaa aatgcgcgcc
    34621 attgtgcacg gttgagatgg cgataccacg ggatattctg ggttgtagta ctcattgtga
    34681 gtctcccgcg gtgggcgatg cccacacgct ttggtataaa aattgtaggg tacagatgcg
    34741 tttatttccc ctcacccggt aggggcgagc gaggggaaac aactcacccg cgctcttgca
    34801 tcaactgctg ggccagcgcc ttcagttctg gcagatattt ttcatctacc ggtccaaacg
    34861 gtttgcggca cagcggcaca gaaacgacat ccatataatg gaggacagtt ttcaggccgc
    34921 ggaatacgcc cgttttgatc agtaaatcaa tgactttatt gcattcagtt tgcagtttct
    34981 gcgcggtctg gatatcgcct tctttcagcg ccttaacgat cccctgatag cgccagccca
    35041 tgatgttgta ggtactaccg ataccaccat cagcgcccgc cagcagacca gaggcgaaga
    35101 tttcgtcgta accgttatag agcacaagat caggatgttc acgacggatc tgctccatct
    35161 gatagagatc gccagaggtc tgtttcagcg cacctacgcc aggcaatgta acaagtgtgt
    35221 tgatctgatc cagggtcagt tttaccccac tcagggctgg aatgttgtac accaccatcg
    35281 gcaaaccatc cgccgaatca ataattgccc gatagtgatc gcagtgttct tcaaagctga
    35341 aaggatagta gaacggcgtg acggcggaga cggcatcgaa gccataacgt ttagccgatg
    35401 ccgcaagttg ttggctttcg gcggtgctga cgcaaccgac gtgggcgatg agtttaatct
    35461 tacctttcgc ctcttcggcg acgatttcca gtacctgttc acgctcggaa aggctttgta
    35521 caaaggcctc gccggtcgaa ccacccacgt ataaaccgtc gatgccctgc tgaatattga
    35581 actgaaccag gcgacgcaga ctcgctttat ccagtgcttg ttgttggtca aaaggagtca
    35641 ggagtgcagc cattacgcca cgtaaattcg ttgccataaa tacctctgaa gtgatgcttg
    35701 tctgataaac gatatacctt tatacctgtt ataccagatc aattaagcaa caccccatac
    35761 agaaagctta taatgcgatc tgcttcacta aagtggcatt atttcttttt gttggtggtc
    35821 tgaccgaaag cgtgccaggt agcagagacg ctgttgagat gcgattgcaa cgcacgatcg
    35881 gcttcgtcag gatcatgacg gcggatcgca tcaacgatcg caatatgctg ttgataacta
    35941 acgttgttat gttcgtgcag tgcctgatcg gtaaccgttg ggcgtgcggc aataagccag
    36001 tcgagcaggg caacgtggat cgccatgaag attgggttac cggggatctc cgccagcacg
    36061 cggtggaaat caacgtctga acgaatgaat gccgcgttgt tatccagcga ctgactgttg
    36121 atttccagtg cttttgccag caaatcgatt tgctcatcgg tggcatgttc agccgcatag
    36181 cgcaccagac tggattcaaa gaacagacgt aattgttcga aatgggcaat cccaccggga
    36241 tgagaaagga aatctttcgc catgccggaa agctcaccga tgatagtgtc cgcagaagga
    36301 cgcgagacgc gagcgcgttc gccgttgttt atttgcacca gacctttgcg ttttaacgct
    36361 gccagcgctt cacgcaccga aggacgcccg acgttaaaga acgccatcag ttcgcgttca
    36421 gacggtaatt gttcaccttc gccaaattca cgacggcgga tcatctgttc cagctcttct
    36481 tccaccattt cggagagttt tttacgcgcc agcgggcggc tacgcaagtt gcgaccaatt
    36541 gcaggtgaag aatcttcggt ttgcgaatca aatgcgttca taaggcccat tctgtaaggt
    36601 cagtgtgatt aacatcatca gtgacatcct atcacaggat tgaaagtagg ggaaaatggc
    36661 aggtttttct ctttgtgcct catcattacc ataattaacg gaataattaa ctattgcgaa
    36721 aaattaatgt aacgcagata aaaacatccc gttttaatta tttataagac tattcacgag
    36781 cattatgaat attatgaatg cgttcttgca aaataatcat aagcgcatat tttttaatga
    36841 aaaattatct cacctacaat taaaaacacg acatccgcac cataaataga cttgcaaaaa
    36901 atataacatc attgttttca atctgccgtt tatgggattg accgttttct tttgacacgg
    36961 agttcaacaa tgttcggcat aattatatct gtcatcgtat taattacgat gggctatttg
    37021 atcctgaaaa actacaaacc tcaggtggtg ctggctgccg caggtatctt cctgatgatg
    37081 tgcggtgtct ggttagggtt cggtggtgta ctcgagcccg ccaaaagcag cggctacttg
    37141 atcgtcgata tttataatga aatcctgcgc atgctgtcca accgcattgc cggattgggg
    37201 ctatcgatta tggcggtggg cggtatgccc gctacatgga gcgcataggg gccagtcgcg
    37261 cgatggtgag cttgttaagc cgcccgttaa aactcattcg ctcaccgtat attattctgt
    37321 cggcaactta cgtcatcggt caaatcatgg cgcagtttat taccagcgcc tccggtctgg
    37381 gtatgttgct gatggtcacc ttatttccga cactggtgag tctgggagta agtcgtctct
    37441 ctgcggtggc ggttatcgca accacaatgt ccattgagtg gggaattctg gaaacgaact
    37501 ccatttttgc agcccaggtc gcgggaatga aaattgccac ttacttcttc cactaccagc
    37561 ttccggtcgc ctcttgcgtc attatctcgg tggcgatctc ccactttttc gtgcaacgcg
    37621 cttttgacaa aaaagataaa aatatcaatc acgaacaagc agagcaaaaa gctctcgata
    37681 atgtcccgcc gctctattac gccattttac ctgtgatgcc gttaatcctg atgctcggct
    37741 cgctgttcct cgcccacgtc gggctgatgc agtcagaact gcatctggtg gtggtgatgt
    37801 tactgagttt gacggtgacg atgtttgttg agttcttccg caagcataac ttgcgcgaaa
    37861 caatggacga tgtgcaggcg ttttttgacg gcatgggtac gcagtttgcc aacgtggtaa
    37921 cgctggtggt cgcgggtgaa atatttgcga aaggcttaac gacgattggc actgtcgatg
    37981 cggttatcag gggtgcggag cattctggtc tgggcggtat tggcgtgatg attattatgg
    38041 cgctggtcat tgccatttgt gccattgtga tgggctctgg caatgcgccg tttatgtcat
    38101 ttgccagtct tattccgaat atcgcagctg gactacatgt accagcggtt gtaatgatta
    38161 tgccgatgca ttttgccacg acgctagcgc gcgcggtttc gccgattact gcggtggtgg
    38221 tcgttacgtc aggaattgca ggcgtttcgc cttttgcggt ggtgaagcga acagcgatcc
    38281 ccatggcagt cggtttcgtg gtgaatatga ttgccacaat cacgctattt tattaagcca
    38341 ttaaaagaca aaacaggcca ccagggcctg tttttgtatt acttcacaac gcgtaatgcc
    38401 ggtcgaccac cgcgtggtgg ctgcggaggt tcatcgtcag gatgagtgtc atcatcgtga
    38461 tctggcttgt cgccatcaat aaccgacata acggtttcgt tgtctgccga tgcctcttca
    38521 tcattcatga tgctggtatc ttcatcgtag gcagcttcag gctcaaacat tgtgcctgcg
    38581 ccattttcac gggcgtagat agccagcacg gcagccagcg gcacagaaac ctgacgcgga
    38641 atgccgccaa agcgcgcgtt aaagcgcacc tcatcattcg ccagttccag attgccgaca
    38701 gcacgcggcg caatgttgag tacgatttgc ccgtcacgcg catattccat aggaacctgc
    38761 acgccaggga gcgtcacatc caccaccagg tgcggcgtga gctggttatc cagcaaccac
    38821 tcatagaatg cacgcagcag atagggacga cgtggtgtta gctgtgacaa atccatacag
    38881 attaactccg gcccagacgc atttcacgtt ctgcttcagt taaagaagca aggaaagagt
    38941 cacgctcaaa gacgcgggtc atatagcctt tcagctcttt cgcacccggg ccgctgaact
    39001 cgatgcccag ttgcggcaga cgccacagca gcggagcaag atagcaatcg accaggctga
    39061 actcatcgct caggaagtac ggcttctgac cgaagaccgg cgcaatcgcc agcagttctt
    39121 cgcgcagttg cttacgtgcg gcatctgctt cagaagctga accgttgatg atggtgttca
    39181 tcagcgtgta ccagtctttt tcgatgcgat gcatgtacag acggctttca ccgcgagcta
    39241 ccgggtaaac aggcatcagt ggcggatgcg ggaaacgctc atccagatat tccataatga
    39301 tgcgagattc ccacagggtc agctcacgat ccaccagggt cggaacgctc tgattcgggt
    39361 tgaggtcaat cagatcctga ggcggattgt ccttttccac gtgttcgatc tcgaaactta
    39421 cacctttctc agccagcaca atgcggacct gatggctata gatgtcagta ggaccggaaa
    39481 acagcgtcat taccgaacgt ttgttggcag cgacagccat gaaaacctcc aggtatagtc
    39541 agaattttta ctgctaccag ccaccaggtg gccagtcaga agttgtgtta cccagtaagg
    39601 aacgactctc tttgttcgaa aatcaaacaa aaaatgagca atacccgaca tttgggcaga
    39661 aaattggatg atagtttacc agattttgcg acctttgtgg tgagtcgatg ccggaaatgg
    39721 ggaaaaagag atgcgcttta gtctgaaata gttgacttag tcccttattg gcgatgtgtt
    39781 ttttgtttta cctgtctgtc aggtggcagc aaaaagcaac tttccagttt ttacgctgat
    39841 tcagatttta gctataaaaa aacccgccga agcgggtttt ttcgaaaatt gttttctgcc
    39901 ggagcagaag ccaattaacg tttggagaac tgcggacgac gacgtgcttt acgcagaccg
    39961 actttcttac gttcaacctg acgagcgtca cgagtaacga agccagcttt acgcagttca
    40021 gaacgcaggg actcgtcgta ttccatcaga gcgcgggtga taccgtgacg gatcgcacca
    40081 gcctgaccag agataccacc acctttaacg gtgatgtaca ggtccagttt ctcaaccatg
    40141 tcgaccagtt ccagcggctg acgaactacc atgcgggcag tttcacgacc gaagtactgt
    40201 tccagagaac gttggttgat tacgatttta ccgttgcccg gtttgatgaa aacgcgagct
    40261 gcggaacttt tgcggcgacc agtgccgtag tattgatttt cagccattgc ctataatccc
    40321 gattagatgt caagaacttg cggttgctgt gccgcgtggt tgtgctcgtt acccgcgtaa
    40381 actttcagtt tacggaacat agcacgaccc agcgggcctt ttggcaacat gcctttaacc
    40441 gcgatttcaa tcacacgctc aggacggcga gcaatcatct cttcaaaggt cgcttgtttg
    40501 ataccaccga tgtggccggt gtggtgatag tacactttgt cagtacactt gttgccggtt
    40561 acagcaactt tgtcagcgtt cagaacgatg atgtaatcac cggtatctac gtgcggagtg
    40621 tattccgctt tgtgcttacc gcgcaggcga cgagccagtt cagtagccag acggcccaga
    40681 gttttaccgg tcgcgtcaac aacataccag tcgcgtttta cggtttctgg tttagctgta
    40741 aaagttttca ttaaaagctt acccaataaa tagttacacg ttggtgaaca cccaaacgtc
    40801 ttcaattgtt gaggttcaca cgacaaagtc cggcaaacct accccttcga atagcctatg
    40861 ccagcacaca aaaagttttg ggaaaaaact ttcttgtaac gtggggtcgc aggattatag
    40921 agaagtcggg gtcaaagatc gacccctttt tgtgatttgt gacaggtttt aacccgccaa
    40981 atgctcgcgc ttcagatact cttcgctttg catctcttgc agacgtgaca ggcaacgctg
    41041 gaactcaaac ttcagccgat cgccctgata aatttcatac agcggcactt ctgcactcac
    41101 cactaatttg acatggcgct cgtaaaactc atccaccagc gcaataaagc gccgcgcttc
    41161 gctctccatc aaccgcgtca taactggtac atcaaacaac atgaccgtat gaaagaggcg
    41221 tgagagcgca atatagtcat gctggctgcg ggcatcgaca cacaaggtgg taaaagagac
    41281 agcaagcgtc tgattctcag cgcccattgt cgccagcggt cgatggttga tttctaacgt
    41341 cggtgaattt tcgcgtttcg ctcccgccag cgccaaccat agtttatcca tttgtgcccg
    41401 ggtttcatcg tggagtggcg aaagccacag atgcgcctga gtgagtgtac gcagtcgata
    41461 atcaacacca gcgtccacgt tcattacatc acaatgctgt ttaatggcat cgattgcagg
    41521 cagaaaacgc gcacgttgca agccatttcg ataaagttcg tccggcggaa tatttgacgt
    41581 tgctaccagg gtaataccgc gggcgaacag ggctttcatc agaccgccaa gtagcatggc
    41641 gtcggtaata tcagaaacaa aaaattcgtc aaaacagagc acgtcagttt cggctttaaa
    41701 gcgatcggca ataatttcca gcggatcggt ctgcccctgt aaggcagtta gctcttcgtg
    41761 cacccgcagc ataaaacggt gaaagtgcag gcgctgtttc cgctctcccg gcaggctttg
    41821 atagaaaagg tccatcagcc aggttttccc gcgtcctaca ccgccccaca tatataagcc
    41881 acgcactggc gtatgctttg tgtcttcgcg tttaccccac agcttaccga cccgcgccat
    41941 tagcccactc gtcctggggg ctggtggcct gctattgatg agttcttgat aaataatttc
    42001 caggcggctg acggcctctt tttgaacgtc gtcgggttga tggctgcctt cattgagcgc
    42061 cttcaggtat tgcgatgttg gggtaacgct ttgcatgatc ttattattat tccttgaata
    42121 atcggtgcgc cgttgttcac ggttgacgaa aaaaaggccg ttctacacta cgcgatatgc
    42181 agtcgggatt ccacttctgt ggaattaacg gttatagtgg cataatcagc cgtaggcatg
    42241 gagcctgaag ccaacaccct acggaaacaa aagacaacgg gagatgttca tgacctggga
    42301 atatgcgcta attgggttag tcgtcggcat cattattggt gctgtggcca tgcgttttgg
    42361 taatcgtaaa ctacgccagc aacaggcgtt gcagtacgaa ctggaaaaga ataaagctga
    42421 actggacgag tatcgcgaag agctggttag ccactttgcc cgcagcgcgg aattactgga
    42481 taccatggcg cacgactatc gccagctgta tcagcacatg gcgaaaagct ccagcagcct
    42541 gctgccggaa ctgtctgctg aagcaaaccc gttccgtaat cgtctggcag agtctgaagc
    42601 cagcaacgat caggcaccgg tgcagatgcc tcgcgactat tctgaaggtg catccggcct
    42661 gctgcgtact ggcgcgaagc gcgactaatt tatttttcgg gcgcagccat tgcgccctcc
    42721 tcttctctcc ctccccgact atcatttaat ctggtgtctc attgttagcc gtctgaaaat
    42781 tcaataacat caaactgttt tgaatctctt ttcttatcat tcaggtacga gagcaggaat
    42841 aatgaaaaaa caaacccagc tgttgagtgc attagcgtta agtgtcgggt taactctctc
    42901 ggcgtcattt caggccgtcg cgtcgattcc aggccaggtt gccgatcagg cccctctccc
    42961 cagtctggct ccaatgctgg aaaaagtgct tccggcagtg gtgagcgtac gggtggaagg
    43021 aacggccagt cagggacaga aaatcccgga agaattcaaa aagttttttg gtgatgattt
    43081 accggatcaa cctgcacaac ccttcgaagg tttaggttcc ggtgtcatca tcaacgccag
    43141 taaaggctat gtgctgacca acaaccatgt gattaatcag gcacagaaaa tcagtattca
    43201 gctcaatgat gggcgcgagt ttgatgcaaa actgattggt agcgatgacc agagcgatat
    43261 cgccctgtta caaattcaaa acccgagcaa attaacgcaa atcgctattg ccgactccga
    43321 taaattgcgc gtcggtgatt ttgccgtagc ggtcggtaac ccatttggcc ttgggcaaac
    43381 cgccacctct ggcattgttt ccgcattagg ccgcagcggg ttgaatcttg aaggtctgga
    43441 aaactttatc cagacagatg cttccattaa ccgcggtaac tccggcggtg cactattaaa
    43501 ccttaacggt gagttaattg gcatcaacac tgcaatcctt gcgcctggcg gcgggagcgt
    43561 cgggattgga tttgccatcc ccagtaatat ggcgcgaaca ctggcgcagc agcttatcga
    43621 ctttggtgaa atcaaacgcg gtttgttagg catcaaaggc accgagatga gtgccgatat
    43681 cgccaaagcc ttcaaccttg acgtgcagcg tggcgcgttt gtcagcgaag tgttgccagg
    43741 ttctggctcg gcaaaagcgg gcgtcaaagc gggcgatatt attaccagcc tcaacggcaa
    43801 accgctgaat agctttgctg agttgcgctc tcgtatcgcg actacagagc cgggcacgaa
    43861 agtgaagctt ggcctgctgc gtaacggcaa accactggaa gtagaagtga cgcttgatac
    43921 cagcacctct tcgtcggcca gcgctgaaat gatcacgcca gcgctggaag gtgcaacgtt
    43981 gagcgatggt cagctaaaag attgcggcaa aggtattaag atcgatgaag ttgtcaaagg
    44041 aagcccagct gctcaggcag gcttgcaaaa agacgatgtg atcattggcg tcaaccgcga
    44101 tcgggtgaac tcgattgctg aaatgcgtaa agtgctggcg gcaaaaccgg ccatcatcgc
    44161 cctgcaaatt gtacgcggca atgaaagcat ctatctgctg atgcgttaat gtcgtaaacc
    44221 gggcatcagg cttacgtgtg atgtccggtt aactcgtggt atgctgctgc cgttcccttt
    44281 tttaatgacg cctccatcat gtttgtgaag ctcttacgtt ccgttgcgat tggattaatt
    44341 gtcggcgcta ttctgctggt tgccatgcct tcgctgcgca gccttaaccc gctttccact
    44401 ccgcaatttg acagtaccga tgagacgcct gccagctata atctggcggt tcgccgcgcc
    44461 gcgccagcgg tggttaacgt ttacaaccgt ggtgtgaaca ccaactctca caaccagctt
    44521 gagatccgca ccctgggatc cggtgtaatc atggatcaac gcggttatat catcaccaat
    44581 aaacacgtca tcaacgacgc cgatcagatc atcgtcgcct tacaggatgg acgtgtattt
    44641 gaagcattgc tggtgggatc tgactctcta accgatctgg cggtacttaa aattaatgcc
    44701 actggcggtt tacctaccat tccaattaat gcacgtcgcg taccgcacat tggcgacgta
    44761 gtactggcga tcggtaaccc gtacaacctc gggcagacca ttacccaagg gattattagt
    44821 gccacggggc gaatcggtct gaaccctacc gggcggcaaa acttcctaca aaccgatgct
    44881 tccattaacc acggtaactc tggcggcgcg ctggtgaact cgctgggaga actgatgggc
    44941 attaacacgc tgtcgtttga taagagtaac gatggcgaaa cgccggaagg tatcggcttt
    45001 gcgattcctt tccagttagc aaccaaaatt atggataagc tgatccgcga tgggcgcgtg
    45061 atccgcggct acattggcat tggtggacgt gagatcgcac cactgcacgc gcagagcggt
    45121 ggtatagatc aactgcaagg gatcgtggtt aatgaagtgt cacctgacgg cccggcggcg
    45181 aatgcgggta ttcaggtcaa cgatctgatt atttcggtgg ataacaaacc ggccatctct
    45241 gctctggaga cgatggatca ggtggcagaa attcgccctg gttcggtgat cccggttgta
    45301 gtgatgcgtg atgataagca gttaacgctg caggtcacca ttcaggaata tccggcaacc
    45361 aattaagtcg agtgctcaaa acaaaaaacc ggagtctgtg ctccggtttt ttattattcg
    45421 ctaatcaatt acttattaac gaactcttcg cccagggcga tatctttctt gagcgtatcc
    45481 agcatacctt ccagcgcgtt ctgttcaaat gcgctcaggg taccgataga tttacgctct
    45541 tccacgccgt ttttacccag cagcagcggt tgagagaaga aacgggcgta ctgaccgtcg
    45601 ccttcaacgt aggcacattc gacaacgcct tgttcgccct gcagtgcacg aaccagagac
    45661 agaccaaaac gtgcagctgc ctggcccata gacagggttg cagacccgcc accggctttc
    45721 gcttcaacca cctcagtacc cgcgttctgg atacgtttgg tcagatcagc cacttcctgc
    45781 tcggtaaaac taacgccagg aacctgtgac agcagcggca gaatggtaac accagagtga
    45841 ccgccaataa ccggcacttc aacttcgcct ggctgtttgc ctttcagttc cgcaacaaag
    45901 gtgttggaac gaatgatatc cagcgtggta acgccgaaca gtttgttttt gtcataaaca
    45961 ccggcttttt tcagcacttc agcagcaatt gcaactgtgg tgttaaccgg gttagtgata
    46021 ataccaatgc acgctttcgg gcaggttttc gcaacttgct gtaccaggtt tttcacgatg
    46081 ccggcgttaa cgttaaacag gtcggaacga tccatacccg gtttacgcgc tacacctgca
    46141 gagataagaa cgacatctgc gccttccagc gccggagtcg catcttcacc agaaaaacct
    46201 ttgattttca cagcagtagg gatatggctc agatcgacag ccacaccggg agtcactgga
    46261 gcgatatcat acagagagag ttctgaacct gaaggcagtt gggtttttaa cagtagtgca
    46321 agcgcctggc caataccgcc agcagcgccg aggactgcga ctttcatcct aaactcctta
    46381 ttatattgat aaactaagat atgttgctcc gctgccgcga ccttaatcca caaaattgcc
    46441 gtttacaatg accacatatc aagaatgtgt agtcacgcaa gtttagcgtt tatgcattta
    46501 attgccgtaa tcaggaacct aacttacgta attaacagtc gcttatgcat tagcgcaaca
    46561 ttccaacagg tggtgacaat ataccctacc gttcagccaa aacaacatca atttgataac
    46621 aattaattta cttttaagca gaatttgcat gccgtgacgc aggcatgttt ctcaataacg
    46681 aaatttgata aaatcccgct ctttcataac attatttcag ccttcttcag ggttgactgt
    46741 ttgcataaaa attcatctgt atgcacaata atgttgtatc aaccaccata tcgggtgact
    46801 tatgcgaagc tcggctaagc aagaagaact agttaaagca tttaaagcat tacttaaaga
    46861 agagaaattt agctcccagg gcgaaatcgt cgccgcgttg caggagcaag gctttgacaa
    46921 tattaatcag tctaaagtct cgcggatgtt gaccaagttt ggtgctgtac gtacacgcaa
    46981 tgccaaaatg gaaatggttt actgcctgcc agctgaactg ggtgtaccaa ccacctccag
    47041 tccattgaag aatctggtgc tggatatcga ctacaacgat gcagttgtcg tgattcatac
    47101 cagccctggc gcggcgcagt taattgctcg cctgctggac tcactgggca aagcagaagg
    47161 tattctgggc accatcgctg gcgatgacac catctttacc acccctgcta acggtttcac
    47221 cgtcaaagac ctgtacgaag cgattttaga gctgttcgac caggagcttt aatctctgcc
    47281 ccgtcgcttc tggcggcggg gaaaatgttg cttatccctc tcaaccccct gctttcccct
    47341 gcgattaatt taacgaatag tgcagtttta ctgcgacatg tcattcacat aatgaataca
    47401 tacggtaaaa aaagcacatt atgcaaaatt cattatctaa ttgaaaatac tagaattaaa
    47461 aataaatgac cgtgttttta attctttttt gttattaaaa ttcacatttt taacacttag
    47521 tatcaactga aacagttagc gtggtattaa ttagttcaat aattagtgta tgcttgattt
    47581 tgtgatatgg gtcacaaaac aaaggcccag ctaaaagatt atgtcgaggt aaaaatcatg
    47641 aaaatcaaaa ccactgtagc tgcattaagc gtactttctg ttctctcttt cggtgcattc
    47701 gctgccgact ccattgatgc tgcacaagca caaaatcgtg aagcaatcgg gaccgtatcc
    47761 gtaagtggtg tggcgtcttc gccaatggat atgcgtgaaa tgctgaacaa aaaagcggaa
    47821 gagaaaggcg caacggccta ccagattact gaagctcgta gcggtgacac ctggcacgct
    47881 acggctgaac tgtacaaata aaccctcatc gtcttgtccg acgatattgc ccccggttcg
    47941 ggggcttttt tttgcgctaa tgacgaacat taaaacgcaa atgcccttcc agctcttcct
    48001 ctgcctcatc aaacagcaat attaacgcgc caaaacggcg acgcgttttc tctcccagat
    48061 gaacaaattc aatctcaagt ggtagcggca ggacatcgtt cattaacaca tcccatagtg
    48121 agtcgagatc gcgtacctta tctttcgcca gaccaaaggt ttggctaaag tcacgataaa
    48181 aatcctcctg actctcaatc tcatcaaaat caaaggtata aatattcatc tattgccacc
    48241 gtcacgtttc gcggcgagtt ctgccgtgca tcgctaagta tagccatgaa aaaaccgaca
    48301 cttttggcgt tggttttgac ctaactatcg gtcaacgcat gttgatactt atggagcata
    48361 cccgccagcc gatgaaccgg ttccgtcacc tgcggtggcg cttgccagat gcgtagcttt
    48421 tcctggtaga tttcaagttc ttccagcaac tggccaaagt accgacgacg tttatcatcg
    48481 ctacgggcag atatcacatg gtctgctgtg cggcgcattt gtcggtgaaa cgccgataaa
    48541 tcctcgttaa ccgggatcgg cgcatcacgc aggcgctggt gcgcgataat catcgtcagc
    48601 gccaggcgaa atttcggcaa atcccctggg aacttattca tcagcaaaaa cagctgctga
    48661 taaagtgccg ggaggtggtt ctctttacga cgtgccacat tggtagtcat cgcggaaaca
    48721 gcggcagaaa caaactgatt aagcagtaca cgtccggtcc tgtcgcgcga tttatcccgc
    48781 acaagcaaaa taacggtgaa cgcgagcaca cagccgacga tttgccctaa tgcgctgtcg
    48841 agaaactgac tgaaatggaa agtcatcggg ttatccagca cgataatatt tatggtgctg
    48901 gccagcgccc ccatcgagcc cagtagccgt tgctgtactt ctataccgag gaaaaatccc
    48961 agcactgcca ggctaataca cagcagcaac atgctctgtt gggtattagg gataatcacc
    49021 aaaaagtaga gcagccctaa cggcagcgcg gccagcgtcc cgtagataaa gtcgatcgcc
    49081 accatgcgtg gattcggcaa acgcattgcc agtgacgtca ctaccgcaat catcaccatt
    49141 gcaccactgc cggaagtcca gcccgtccac agccagaaaa gcgtgcccag aatgcaggaa
    49201 agtgtggttc gccagaagtt aaccatcgca tgatgacgtt cggctgactc tactttgact
    49261 tccggttcgc cttgcaggat ctcttcttcg gtggcgttga tttttgtgtt actgataacg
    49321 ccgcgcttga gaagctgata acgcgttgcc gccgcgaccc agctataaat ggtgacaggc
    49381 gtttcccgtt ccccggtcca ggcgataact ctccgcaggc gtttgagctg cttgtggacg
    49441 tcctgcacgg tttctaccgg cgtgtcaaaa aattcgcgga aagtatcagt gatcaattcc
    49501 gggcgcgtat tctgaataag ataagtttcg caggattggg taatcagcgt cagcgatagc
    49561 gtattgatcg cttttaaacg tcgattggcc cgcgcccagc gggaagattc catattcagg
    49621 ttgctgcgca tgccttgtag ccccgtggtg cgtcgcacca ggtcgcccca ggctttatcg
    49681 acaacttcac catcgccatg cttgatacag agttgcatta attgatattg cgcgaccagc
    49741 aaactttcca gctctcgatc cacttcttgt ttgatcgatc gcggagaaaa gagcaaatcc
    49801 gctataatcg cacacacaat accgataacg atctcgctac aacgttcgac ggcaaactgc
    49861 ggcgtaagca atggttccgg ctgaatggtg atcacaatga tcagcgcggt ataaccggcc
    49921 agcccccacg cgtacgagtt ttctattcgt accagcgagg atatccaggt acaaaaaccg
    49981 gcccagatac agcacaccag aatcatcaat aatggtgcgc ggatcatcgc aatgatgatc
    50041 accagtccgg caatacagcc aataaatgtg ccgatgatgc gcaaaaagcc acgataacga
    50101 atagcgccag aatacggttc acctcccgca gcaaaggccg gaccggcggc aacaatcgcc
    50161 gctgtcagta ccgcccagcg tggcgtttcc agctggaagt gaaagccaac aaacagcgcc
    50221 agtacaatgg cggtcgccag ttttaccgca aagcgaatat gttggttagc aatggagaaa
    50281 atacccatcg tgattaacca aactcacgca ggcgatgggc cattttacgg aagaacgaat
    50341 cctggctttc gtcgcgatct tgtttgccag tgaccaccac tgtagcagtg gtgcccgcag
    50401 gccagatgtt ctcttgctgg ttgtcgagac gaatacgaac cggaacacgt tgcgcaagac
    50461 gcacccattc aaggttagag tctatagtcg ccatcccttt gtcgtcacgc gtgctgctgg
    50521 cgttggtgac ccctgcggca acactatcaa cagtcccttt cagcactttg ttactgccaa
    50581 gcggcgtgat ctctgcacga taccccggac gcaccccttc cagcttagtt tcttccatat
    50641 aggccagtac atagaaggag ttctgtttca ccagcgcaac cgccgttgat cctcgagtaa
    50701 taaactcacc ggtatagacg ttgaggttgg tcacccagcc atctgctggc gcgcggatca
    50761 ccgtgcgttc aagatccagt tttgccagat cgcgggtcgc ctgcgctttc gctaactgat
    50821 gcagaaccgt ttgtagtacg ttgttagcct agtcgatctc ttcgcgagac atcgcctgca
    50881 caccgagacg gttacgacgt ccggcctcct ggcgtttctc ctgtgccagt acctgataat
    50941 aagcaacatc ggcttgcgct tcctcaagcg ccttttgata gcgcggctgg tcgatggtga
    51001 acagtatctg tccttttttc accagctggt tatcatgaac attcacctgg gtaatgagtc
    51061 cagaaacgtc cggcgcgatc gcaatgacgt cagcgctaaa gcgcgcgtca cgcgtccagg
    51121 gggattcggt gtaatagacc caggcattaa aaattgcgat gaaggccaga atgactaata
    51181 cgaccgtgat ggccgtacgg gagaattttc ttattagtgt tttcacttca acctcaaacg
    51241 aacagtcgcg atatcaaata aaacaagcag caatagagcg cggtgttgaa caacgccgga
    51301 tgccagacaa agtcgtagat acctgttggc acaagtaccc ggcgcaccag ccagaaaatc
    51361 gccagtgata aaagcaattc aaaaaatatc ggtgggaagg acagcccaaa caccacgata
    51421 acgggaaaca gactcatgtt gaccttggtt gtatagagag agcaggcgtt attattttca
    51481 gcatctgtcg ccgcagagaa gggcatggaa agccgggcga gagcaacatt gctgtagatt
    51541 gatatttaat atattagcgt aactgttatg ctgttatcta
//
LOCUS       DADLFA010000006        47217 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000006 DADLFA010000000
VERSION     DADLFA010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47217
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.006"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            27..764
                     /locus_tag="JQH65_000311"
     CDS             27..764
                     /locus_tag="JQH65_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HAY6137831.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGKASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYDGLSAKKEQNLP"
     gene            875..1885
                     /gene="pyrD"
                     /locus_tag="JQH65_000312"
     CDS             875..1885
                     /gene="pyrD"
                     /locus_tag="JQH65_000312"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HAY6137832.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            2059..2601
                     /gene="zapC"
                     /locus_tag="JQH65_000313"
     CDS             2059..2601
                     /gene="zapC"
                     /locus_tag="JQH65_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HAY6137833.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNVLVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(2598..3707)
                     /gene="ycbX"
                     /locus_tag="JQH65_000314"
     CDS             complement(2598..3707)
                     /gene="ycbX"
                     /locus_tag="JQH65_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HAY6137834.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     ILEGEVSPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            3951..6059
                     /gene="rlmKL"
                     /locus_tag="JQH65_000315"
     CDS             3951..6059
                     /gene="rlmKL"
                     /locus_tag="JQH65_000315"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HAY6137835.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYSDWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGALSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            6071..7978
                     /locus_tag="JQH65_000316"
     CDS             6071..7978
                     /locus_tag="JQH65_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY6137836.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIVLIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNIAEGKQEVMVNGKPRHVLSYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            8108..9361
                     /gene="pqiA"
                     /locus_tag="JQH65_000317"
     CDS             8108..9361
                     /gene="pqiA"
                     /locus_tag="JQH65_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HAY6137837.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            9366..11006
                     /gene="pqiB"
                     /locus_tag="JQH65_000318"
     CDS             9366..11006
                     /gene="pqiB"
                     /locus_tag="JQH65_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HAY6137838.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENTDVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            11003..11566
                     /gene="pqiC"
                     /locus_tag="JQH65_000319"
     CDS             11003..11566
                     /gene="pqiC"
                     /locus_tag="JQH65_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HAY6137839.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSVQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            11822..11989
                     /gene="rmf"
                     /locus_tag="JQH65_000320"
     CDS             11822..11989
                     /gene="rmf"
                     /locus_tag="JQH65_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HAY6137840.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(12059..12577)
                     /gene="fabA"
                     /locus_tag="JQH65_000321"
     CDS             complement(12059..12577)
                     /gene="fabA"
                     /locus_tag="JQH65_000321"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HAY6137841.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(12646..14406)
                     /locus_tag="JQH65_000322"
     CDS             complement(12646..14406)
                     /locus_tag="JQH65_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HAY6137842.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            14592..15044
                     /gene="matP"
                     /locus_tag="JQH65_000323"
     CDS             14592..15044
                     /gene="matP"
                     /locus_tag="JQH65_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HAY6137843.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKLDLQALLGKE"
     gene            complement(15120..16160)
                     /gene="ompA"
                     /locus_tag="JQH65_000324"
     CDS             complement(15120..16160)
                     /gene="ompA"
                     /locus_tag="JQH65_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HAY6137844.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(16517..17026)
                     /gene="sulA"
                     /locus_tag="JQH65_000325"
     CDS             complement(16517..17026)
                     /gene="sulA"
                     /locus_tag="JQH65_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HAY6137845.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            17245..17874
                     /gene="sxy"
                     /locus_tag="JQH65_000326"
     CDS             17245..17874
                     /gene="sxy"
                     /locus_tag="JQH65_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HAY6137846.1"
                     /translation="MKSLSYKRIYKSQEYLAMLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(17837..19999)
                     /gene="yccS"
                     /locus_tag="JQH65_000327"
     CDS             complement(17837..19999)
                     /gene="yccS"
                     /locus_tag="JQH65_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HAY6137847.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGFWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(20009..20455)
                     /locus_tag="JQH65_000328"
     CDS             complement(20009..20455)
                     /locus_tag="JQH65_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAY6137848.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            20578..22632
                     /gene="helD"
                     /locus_tag="JQH65_000329"
     CDS             20578..22632
                     /gene="helD"
                     /locus_tag="JQH65_000329"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HAY6137849.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GKVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(22664..23122)
                     /gene="mgsA"
                     /locus_tag="JQH65_000330"
     CDS             complement(22664..23122)
                     /gene="mgsA"
                     /locus_tag="JQH65_000330"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAY6137850.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(23218..23880)
                     /locus_tag="JQH65_000331"
     CDS             complement(23218..23880)
                     /locus_tag="JQH65_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HAY6137851.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            24053..24466
                     /locus_tag="JQH65_000332"
     CDS             24053..24466
                     /locus_tag="JQH65_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAY6137852.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(24511..24828)
                     /gene="hspQ"
                     /locus_tag="JQH65_000333"
     CDS             complement(24511..24828)
                     /gene="hspQ"
                     /locus_tag="JQH65_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HAY6137853.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(24886..26076)
                     /gene="rlmI"
                     /locus_tag="JQH65_000334"
     CDS             complement(24886..26076)
                     /gene="rlmI"
                     /locus_tag="JQH65_000334"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HAY6137854.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALVSALQTLYPECAIYDRSDVA
                     VRKKEGMELTLGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            26171..26449
                     /gene="yccX"
                     /locus_tag="JQH65_000335"
     CDS             26171..26449
                     /gene="yccX"
                     /locus_tag="JQH65_000335"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAY6137855.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(26446..26775)
                     /gene="tusE"
                     /locus_tag="JQH65_000336"
     CDS             complement(26446..26775)
                     /gene="tusE"
                     /locus_tag="JQH65_000336"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HAY6137856.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(26866..27525)
                     /gene="yccA"
                     /locus_tag="JQH65_000337"
     CDS             complement(26866..27525)
                     /gene="yccA"
                     /locus_tag="JQH65_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HAY6137857.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(27732..27819)
                     /locus_tag="JQH65_000338"
     tRNA            complement(27732..27819)
                     /locus_tag="JQH65_000338"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27783..27785),aa:Ser,seq:tga)
     gene            28246..29364
                     /gene="hyaA"
                     /locus_tag="JQH65_000339"
     CDS             28246..29364
                     /gene="hyaA"
                     /locus_tag="JQH65_000339"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HAY6137858.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            29361..31154
                     /gene="hyaB"
                     /locus_tag="JQH65_000340"
     CDS             29361..31154
                     /gene="hyaB"
                     /locus_tag="JQH65_000340"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HAY6137859.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            31173..31880
                     /gene="hyaC"
                     /locus_tag="JQH65_000341"
     CDS             31173..31880
                     /gene="hyaC"
                     /locus_tag="JQH65_000341"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HAY6137860.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDGTVISTMV
                     NGYRSHKFGKISNKECS"
     gene            31877..32464
                     /locus_tag="JQH65_000342"
     CDS             31877..32464
                     /locus_tag="JQH65_000342"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HAY6137861.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLLAAEQSALAQLAAWGSVP
                     QLANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            32461..32859
                     /gene="hyaE"
                     /locus_tag="JQH65_000343"
     CDS             32461..32859
                     /gene="hyaE"
                     /locus_tag="JQH65_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HAY6137862.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGVFRFPTTLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            32856..33713
                     /locus_tag="JQH65_000344"
     CDS             32856..33713
                     /locus_tag="JQH65_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HAY6137863.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            33847..35391
                     /gene="appC"
                     /locus_tag="JQH65_000345"
     CDS             33847..35391
                     /gene="appC"
                     /locus_tag="JQH65_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HAY6137864.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWAFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYLAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            35403..36539
                     /gene="appB"
                     /locus_tag="JQH65_000346"
     CDS             35403..36539
                     /gene="appB"
                     /locus_tag="JQH65_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HAY6137865.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLSSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            36552..36644
                     /gene="cbdX"
                     /locus_tag="JQH65_000347"
     CDS             36552..36644
                     /gene="cbdX"
                     /locus_tag="JQH65_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HAY6137866.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            36724..38022
                     /locus_tag="JQH65_000348"
     CDS             36724..38022
                     /locus_tag="JQH65_000348"
                     /EC_number="3.1.3.2"
                     /EC_number="3.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AppA family phytase/histidine-type acid
                     phosphatase"
                     /protein_id="HAY6137867.1"
                     /translation="MKAILIPFLSLLIPLTSQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYFGHYQRQRLVADGLLAKKGCPQP
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(38137..40317)
                     /gene="etk"
                     /locus_tag="JQH65_000349"
     CDS             complement(38137..40317)
                     /gene="etk"
                     /locus_tag="JQH65_000349"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HAY6137868.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(40337..40783)
                     /gene="etp"
                     /locus_tag="JQH65_000350"
                     /gene_synonym="yccY"
     CDS             complement(40337..40783)
                     /gene="etp"
                     /locus_tag="JQH65_000350"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HAY6137869.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(40771..41910)
                     /locus_tag="JQH65_000351"
     CDS             complement(40771..41910)
                     /locus_tag="JQH65_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HAY6137870.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTRNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(41956..44052)
                     /locus_tag="JQH65_000352"
     CDS             complement(41956..44052)
                     /locus_tag="JQH65_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HAY6137871.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVNDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(44052..44798)
                     /locus_tag="JQH65_000353"
     CDS             complement(44052..44798)
                     /locus_tag="JQH65_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HAY6137872.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVDPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(44795..45439)
                     /gene="gfcB"
                     /locus_tag="JQH65_000354"
     CDS             complement(44795..45439)
                     /gene="gfcB"
                     /locus_tag="JQH65_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HAY6137873.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(45546..45851)
                     /locus_tag="JQH65_000355"
     CDS             complement(45546..45851)
                     /locus_tag="JQH65_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-rich inner membrane protein GfcA"
                     /protein_id="HAY6137874.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(46293..46505)
                     /gene="cspH"
                     /locus_tag="JQH65_000356"
     CDS             complement(46293..46505)
                     /gene="cspH"
                     /locus_tag="JQH65_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HAY6137875.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            46791..47003
                     /gene="cspG"
                     /locus_tag="JQH65_000357"
     CDS             46791..47003
                     /gene="cspG"
                     /locus_tag="JQH65_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HAY6137876.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            47014..47202
                     /locus_tag="JQH65_000358"
     CDS             47014..47202
                     /locus_tag="JQH65_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAY6137877.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVTLQIRTDHAPLDFTK"
BASE COUNT        11625 a        11730 c        12413 g        11449 t
ORIGIN      
        1 tagcgatgtg cagtcgggta aagtgaatgc cagcgcgaca tttgtacttc attatgacta
       61 atacgtggaa cagattggcg ctcttgattt tcgccgtttt atcgctgctg gtggcgggtg
      121 aattgcgggc aggagtggtg gtcggcggaa cgcgatttat ctttccggca gacagagaat
      181 cgatatctat tttactgact aataccagtc aggaatcctg gctgattaac agtaaaatca
      241 accgcccaac gcgttgggcc ggggggaaag cgtcgacagt gccagcacca ttactggccg
      301 ctccgccact tattctcctg aagcccggta ctacaggcac gttgcgcttg ctgagaacgg
      361 aaagcgacat cttgcctgtg gatcgcgaaa cgctatttga gttaagcatt gccagcgtgc
      421 catccggcaa agttgaaaat cagagcgtaa aagtggcgat gcgctcggta tttaaactgt
      481 tctggcgacc cgaaggtttg ccgggcgacc cgctggaagc ttatcaacaa ttacgctgga
      541 cacggaattc gcagggtgta caactcacta acccaacgcc ttactacatt aacctgattc
      601 aggtgagcgt aaatggtaaa gcgttaagta atgcgggagt tgtgccgcct aaaagccagc
      661 gtcagacaag ctggtgtcag gcaatcgcac cgtgtcatgt cgcctggcgg gcgataaatg
      721 attacgacgg gttgagtgca aagaaggagc aaaatctgcc ctgaaacagg ttcggaaaac
      781 gtttgcgttt tttttgccgc aggtcaattc ccttttggtc cgaactcgca cataatacgt
      841 ccccggtttg cacaccggga atccaggaga gttcatgtac taccccttcg ttcgtaaagc
      901 ccttttccag ctcgatccag agcgcgctca tgagtttact tttcagcaat tacgccgtat
      961 tacaggaacg ccgtttgaag cactggtgcg gcagaaagtg cctgcgaaac ctgttaactg
     1021 catgggcctg acgtttaaaa atccgcttgg tctggcagcc ggtcttgata aagacgggga
     1081 gtgcattgac gcgttaggcg cgatgggatt tggatcgatc gagatcggta ccgtcacgcc
     1141 acgtccacag ccaggtaatg acaagccgcg tctctttcgt ctggtagatg ccgaaggttt
     1201 gatcaaccgt atgggcttta ataatcttgg cgttgataac ctcgtagaga acgtaaaaaa
     1261 ggcccattat gacggcgtcc tgggtattaa catcggcaaa aataaagata cgccagtgga
     1321 gcagggcaaa gatgactatc tgatttgtat ggaaaaaatc tatgcctatg cgggatatat
     1381 cgccatcaat atttcatcgc cgaatacccc aggattacgc acgctgcaat atggtgaagc
     1441 gctggatgat ctcttaaccg cgattaaaaa taagcaaaat gatttgcaag cgatgcacca
     1501 taaatatgtg ccgatcgcag tgaagatcgc gccggatctt tctgaagaag aattgatcca
     1561 ggttgccgat agtttagttc gccataatat tgatggcgtt attgcaacca ataccacact
     1621 cgatcgttct cttgttcagg gaatgaaaaa ttgcgatcaa accggtggct taagtggtcg
     1681 tccgcttcag ttaaaaagca ccgaaattat tcgccgcttg tcactggaat taaacggtcg
     1741 cttaccgatc atcggtgttg gcggcatcga ctcggttatc gctgcgcgtg aaaagattgc
     1801 tgcgggtgcc tcactggtgc aaatttattc tggttttatt tttaaaggtc cgccgctgat
     1861 taaagaaatc gttacccata tctaattatt tcttcgaatt cctctttata agataatcag
     1921 ggctttattt tcagccctgg ttgttttata ttcatcgctg ttgcttattt agacattttg
     1981 tacttttatt gttgaggtta ttaagcgaag cgacaatgga ttgtgttgct gcggttttat
     2041 agggtagggg agaggcagat gcgaattaaa ccagacgata actggcgttg gtattacgat
     2101 gaagagcacg atcgaatgat gctcgattta gccaatggta tgctatttcg ctcacgtttt
     2161 gcgcgcaaga tgttgactcc agatgccttt tctcccgcag gcttttgcgt tgacgacgcc
     2221 gcgctctatt tctcttttga agaaaagtgc cgcgatttta atttatctaa agaacaaaaa
     2281 gccgaactgg tgctgaatgt actggtagca attcgctatc tcaaaccgca aatgccgaaa
     2341 agctggcatt ttgtttccca tggtgaaatg tgggtgccca tgccgggcga tgcggcctgc
     2401 gtttggttga gcgatactca cgagcaggtc aatttgctgg tcgttgaatc tggcgaaaat
     2461 gccgcactat gcctgctggc acaaccctgc gttgttattg cgggtcgcgc gatgcagttg
     2521 ggcgacgcca ttaaaatcat gaacgacagg ctgaaaccgc aggttaatgt tgacagcttc
     2581 agcctcgaac aggcagtcta acgcgccaac ttaagtgcag tcttcggtac acagctacag
     2641 caaagaatgg tgccatcatc gcccattgct gattttttca gcggcgagac ttcgccttct
     2701 aaaatctgaa cacggcaact tccacaaatg cccgcgcggc aagaataagg gatacgaatt
     2761 ccctgatttt ctaattgctc cagcaacacc tgttggttat ttccacgaaa tgcctgtccc
     2821 tgccagtcaa tatctacatt tgcgtccggt tgttgcgtga tgttggcggt atcatcagcg
     2881 gcagctgcgc cgtaaatttt tgccggagcc gttgccagaa tttccacctc atcgccaacg
     2941 cgaatcacgc cgctattacg ggcaattaaa ttctgaccaa aatcgacatc gccgttatcc
     3001 tgggcagtgc ggaaagattg caatgttttt aatggttcgc ctgccggatg tttttgccct
     3061 ttttctgggc tgacggtggt gaaaatacag cggctacaag gtttaaccac atcaaacacc
     3121 acatcaccaa tgcgaatcac tttccagcga tcttcttccc aggctgacgc gccggaaacc
     3181 accagattgg ggcggaattg ctccattttt acactggccg gacaacgttg ttggagatca
     3241 cgtaacgagg cttcgttagc aagaaggtaa ggatagccat cagcaaatga cagaggtaca
     3301 gtgttgtggc gtttcacgcg ccgggtcatt tgtggcccca cccagcgtaa ttgcacttcg
     3361 cgggagaaaa atccacttag ccatttgttg atcgcgtctg gcgcaattcg cgcggtaaaa
     3421 tgtgtgcccc aaacttcggt tggtgcgtct tgtgtggcga aatcagcaaa acgaacatat
     3481 gcgctactgc catctggtgc ggtgagatgc aagccatcat gcacaggtga aggagtaaac
     3541 cgtaccatct ggggaaactg gcgagcggta ataaacgtac cgtcaggttc cgtgatcata
     3601 aagatgcgat cgaaggccag accactgaca tcagccagag catgtgtaag accaatgccg
     3661 cgcatcgatt taacaggatg aataaaaagc cgggttaatg tcgccacggt gcggtcctca
     3721 aatgaaaata agccctcaac tttatgacat gacgcgctta ttagctataa tgcgcaacaa
     3781 ttttcttagc gcctgtccca ttaggctatt ttatttgtca gtttggccct gggttgtgct
     3841 cgaaatcctc acgtacttgt gtacgctccg gtttctccgc gctgtccatg tccagactga
     3901 ctgcaacaat tacgcctact gcggtaggct cttagagtaa aagtgacgat atgaattctc
     3961 tgtttgccag tacggcccgt gggctggaag agctgttaaa aactgaactg gaaaacctgg
     4021 gggccgttga atgccaggtg gttcagggtg gggtccattt caagggcgac acacggcttg
     4081 tttaccagag cctgatgtgg agccgcctgg cctcgcgtat tatgttgccg ctgggcgagt
     4141 gtaaggttta cagcgattta gacctctatc tcggtgttca ggcgatcaac tggacagaga
     4201 tgtttaatcc tggcgcgacc ttcgctgtcc acttcagtgg tttgaatgac accatccgca
     4261 acagtcagta cggcgcgatg aaagtgaaag acgcgatcgt cgatgctttc acgcggaaaa
     4321 atctgccgcg tccaaatgtt gatcgcgatg cgccggatat ccgcgttaac gtctggctgc
     4381 ataaagaaac cgccagtatc gctcttgatc tgagtggtga tggtttacat ctgcgtggct
     4441 atcgcgatcg tgctggtatt gcgccgatca aagaaaccct ggcagccgct attgtgatgc
     4501 gatccggctg gcagcctgga acaccgctgc tcgatccgat gtgtggttcc ggtacgttgc
     4561 tgattgaagc ggcgatgctg gcgaccgatc gcgcacccgg cttgcaccgt gggcgttggg
     4621 gctttagcgg ctgggcgcag catgatgaag ctatctggca ggaagtgaaa gcggaagcgc
     4681 aaactcgcgc ccgtaaaggc ctggctgagt atagctctca tttttacggt tcggacagcg
     4741 acgcacgggt gattcaacgt gcacgcacta acgcccgtct tgcggggatt ggtgaactga
     4801 tcacctttga ggtgaaagat gtcgcgcaac tgaccaatcc gctgccgaaa gggccgtacg
     4861 gcacagtgtt gagcaacccg ccatacgggg aacgtctgga cagcgaaccg gcgctgattg
     4921 cgctgcatag cctgctgggt cggatcatga aaaaccagtt tggtggctgg aatctctctt
     4981 tgtttagtgc ctcgccggat ctgctaagct gcttgcagct gcgtgcagac aaacagtaca
     5041 aggcgaaaaa cggcccgctg gactgcgtac agaaaaatta ccacgttgcc gaaagcactc
     5101 cagacagcaa accggcgatg gtagcggaag actacgccaa ccgcctgcgt aagaacctca
     5161 aaaaattcga gaagtgggct cgccaggaag gaattgaatg ttaccgcctg tatgacgccg
     5221 atctgccgga atataacgtt gccgttgacc gttattccga ctgggtggtg gtgcaggagt
     5281 atgcaccgcc aaaaactatt gatgctcaca aagcgcgtca gcgtctgttc gatattatcg
     5341 ctgcaaccat ttcggtactg gggattgcgc caaacaaact ggtgctgaaa acccgtgaac
     5401 gccagaaggg caaaaaccag taccagaaac tgggcgagaa gggcgagttt cttgaagtta
     5461 ccgaatataa cgctcacttg tgggtgaacc tgacggatta tctcgatact ggtctgttcc
     5521 tcgatcaccg tatcgcccgc cgtatgctcg gtcagatgag caaaggcaaa gatttcctca
     5581 acctgttctc ttataccggt agcgccaccg tgcatgcggg attaggcggc gcgctcagca
     5641 ccaccaccgt ggatatgtcg cgtacttatc tggagtgggc agaacgcaac ctgcgtctga
     5701 atggcctaac cgggcgtgcg catcgcctga ttcaggccga ttgcctggcg tggctgcgtg
     5761 aggcaaatga acagtttgat ctgatcttta tcgatccgcc aaccttctct aactcaaaac
     5821 gaatggaaga tgcgtttgat gttcagcgcg atcatctggc gctgatgaaa gatttgaaac
     5881 gtctgctgcg tgcaggtggg acgatcatgt tctcgaacaa caaacgtggc ttccgtatgg
     5941 atctcgacgg cctggcaaaa ctgggactga aagcacaaga aattacgcaa aaaacgctct
     6001 cccaggattt cgcccgtaac cgccagatcc acaactgctg gctgattacc gcagcctgaa
     6061 aggaatagta atgtcattaa tcagtatgca tggcgcatgg ctgtcgttca gcgacgcgcc
     6121 gcttctcgat aacgcagaac tgcatatcga agataacgaa cgtgtttgtc tggtgggccg
     6181 caacggcgcg ggcaaatcga cgttaatgaa aatcctcaac cgtgaacaag ggctggatga
     6241 cggtcgcatt atttacgagc aagatttgat tgtagctcgt ctgcaacagg atccgccgcg
     6301 taacgttgag ggtagcgttt atgatttcgt tgccgaaggc attgaagaac aagcggaata
     6361 tctgaaacgc tatcacgata tttcgcgcct ggtgatgaac gacccgagcg agaaaaatct
     6421 caacgaactg gcgaaggttc aggaacagtt ggatcaccac aacctgtggc agctggaaaa
     6481 ccgcatcaac gaagtgctgg cgcaactggg gttagatcct aatgttgcgc tgtcgtcgct
     6541 ttccggcggc tggttgcgta aagcggcatt aggacgcgcg ctggtgagta atccgcgcgt
     6601 gctgttgctt gatgaaccga caaaccacct ggatattgaa accatcgact ggctggaagg
     6661 gttccttaaa accttcaatg ggacgattat tttcatctcc cacgaccgtt cgtttatccg
     6721 caatatggcg acgcgcattg ttgatctcga tcgcggcaag ctggtgacct atccagggaa
     6781 ttacgaccag tacctgcttg aaaaagaaga agccctgcgc gtggaagaac tacaaaatgc
     6841 cgagttcgat cgcaaactgg cgcaggaaga ggtgtggatc cgccagggga tcaaagcacg
     6901 ccgtacccgt aatgaaggcc gcgtacgcgc cctgaaagca atgcgtcgcg agcgtggtga
     6961 acgtcgcgaa gtgatgggta ccgcaaagat gcaggtggaa gaggccagcc gctccggtaa
     7021 gatcgttttc gaaatggaag acgtttgcta ccaggttgac ggtaagcaac tggtgaaaga
     7081 tttttcggcc caggttctac gtggcgacaa aattgtcctg attggtccga atgggtgcgg
     7141 caaaaccacg ctgctgaaac tgatgctcgg tcagcttcaa gcggacagcg ggcgtattca
     7201 cgttggcacc aaactggaag tggcttattt cgatcagcac cgcgcggaac tggatcccga
     7261 taaaacggtg atggataaca ttgccgaagg taagcaagag gtgatggtta acggcaagcc
     7321 acgccacgta ttgagctatt tgcaggactt tctgttccat ccgaaacggg cgatgacgcc
     7381 ggtacgtgcg ctttctggcg gtgagcggaa ccgcttgctg ctggcgcgtt tgttcctcaa
     7441 accaagcaac ttattgattc ttgacgaacc gaccaacgat cttgatgtcg aaacgctgga
     7501 actgctggaa gaactgatcg acagctatca gggcacggta ttgctggtta gccacgatcg
     7561 tcagtttgtc gataacaccg ttacagaatg ttggatcttc gaaggcggcg gtaaaattgg
     7621 tcgttatgtc ggcggttatc atgatgcccg tggtcagcaa gagcagtatg tggcgctcaa
     7681 acagcctgcg gtgaaaaaaa ccgaagaagc cgccgcggca aaagcagaaa ctgtaaaacg
     7741 cagcagtagc aaactaagct ataaattgca gcgcgaactg gagcagctac cgcaattgct
     7801 cgaagatctg gaggcgaagc tggaagccct acagacgcag gtggcggatg cttccttctt
     7861 cagtcagcca catgagcaga cacaaaaagt gctcgccgat atggctgctg cagagcagga
     7921 gctggagcaa gcctttgaac gctgggagta tcttgaagcg ttaaaaaatg gtggctgatc
     7981 gcaaaatagt cgatttaaac agagacagct gacaacggta aaattgcctg atgaactggg
     8041 tttatcaggc atgaaaacca acactttgtt agtcatctgt aaaatagcgc atcattaagg
     8101 agtaccaatg tgcgaacatc atcatgccgc gaagcacatc ctgtgctcgc agtgtgacat
     8161 gctggtggcg ttaccgcgcc ttgagcatgg tcagaaagcg gcatgtcccc ggtgtggcac
     8221 aacgttaacc gtggcgtggg atgcccctcg gcagcgtccg accgcctatg cgttggctgc
     8281 actgttcatg ctgttgctgt ccaacttgtt tccttttgtg aatatgaacg ttgcaggagt
     8341 taccagtgaa attacattac tggaaattcc cggcgtgctt ttttctgagg actacgccag
     8401 cctcggcacc tttttcctgt tgtttgtgca actggttccc gcgttttgtc tgataaccat
     8461 tctgttactg gtgaatcgcg cggaattacc ggtccgttta aaagagcaac tggcacgggt
     8521 gctttttcaa ctcaaaacct ggggaatggc ggagattttc ctcgcgggtg tgctggtcag
     8581 tttcgttaaa ctgatggctt acggcagcat tggggtaggc agcagctttc tcccctggtg
     8641 tttattttgt gtcctgcaac tgcgcgcttt tcagtgcgtt gatcgtcgct ggttatggga
     8701 cgacatcgcc ccgatgccag aactgcgcca gccgctaaaa ccaggcgtca cggggatacg
     8761 tcaggggctg cgttcgtgct cctgttgtac ggcaatcctt cctgctgatg aacccgtgtg
     8821 cccgcgttgt agtaccaaag ggtacgttcg gcgtagaaac agcctgcagt ggacactcgc
     8881 gctgcttgta acgtccatca tgctgtatct tccggctaat attttgccca tcatggtgac
     8941 ggatttatta ggctcgaaga tgccgtcgac gattctcgct ggggtcattc tgttatggag
     9001 cgaaggctct tatcccgtcg ctgcggtgat ctttctggcc agtattatgg tgccaacgtt
     9061 aaagatgatc gccatcgcgt ggctgtgttg ggatgccaaa gggcatggca agcgcgacag
     9121 tgaaagaatg catttgattt atgaagttgt tgagtttgta ggccgctggt cgatgattga
     9181 cgttttcgtt atcgcggtgc tctcggcgct ggtgcgtatg ggaggtttaa tgagtattta
     9241 tccggcaatg ggtgcattaa tgtttgcttt agtcgtcata atgacaatgt tttctgctat
     9301 gacgtttgac ccgcgtttgt cgtgggatcg tcaacctgaa tcagagcatg aggagtcctg
     9361 acagtatgga atctaataat ggggaagcca aaatccagaa agtgaagaac tggtctcccg
     9421 tgtggatatt tcctatcgtc acggcgctca ttggggcctg ggttctattt tatcattaca
     9481 gccatcaggg accggaagtg accctgatca ccgcgaatgc ggaaggaatt gaaggtggca
     9541 aaaccaccat taaaagccgt agcgttgacg tcggcgtggt tgaaagcgcc acactggctg
     9601 atgatttgac gcacgttgaa atcaaagcgc ggctgaattc cggtatggaa aaattgctgc
     9661 ataaagacac cgtcttttgg gtggtgaaac cgcagattgg tcgcgaaggg attagcggcc
     9721 tgggaacgct gctgtctgga gtttatatcg aactgcagcc aggcgcgaaa ggcagcaaaa
     9781 tggataaata cgatttgctg gactcgccac cgttggcccc gcctgatgcg aaaggtatcc
     9841 gtgtgattct cgatagcaaa aaagccgggc agctctcgcc aggagatccg gtgctgttcc
     9901 gtggctatcg ggtaggttcg gttgaaacca gcaccttcga tacgcaaaaa cgcaatatca
     9961 gttatcaact gttcatcaat gcaccttatg accgactggt gaccagcaat gttcgcttct
    10021 ggaaagatag tggcattgcg gttgatctga cgtcagcggg gatgcgtgtg gagatgggct
    10081 cattgacaac gctgcttagt ggcggtgtta gctttgatgt gccggaaggt ctggatttag
    10141 ggcagccagt ggcaccgaaa acagctttcg ttttgtatga tgatcagaag agcattcagg
    10201 attcgttgta caccgatcac attgattatc tgatgttctt taaagattcg gtacgcggtc
    10261 tgcaaccggg agctccggta gaattccggg gtattcgcct gggtaccgta agcaaagtgc
    10321 cattctttgc gccgaatatg cgtcagacat ttaacgatga ttaccgtatt ccggtactga
    10381 ttcgtatcga gccagagcgg ctgaaaatgc agcttggcga aaatacggat gttgttgagc
    10441 accttggcga attgttgaaa cgtggtttac gcggatcgct gaaaaccgga aacctggtca
    10501 ctggtgcact gtatgttgat ctcgatttct atccaaatac gcctgcaata accggtattc
    10561 gtgaatttaa tggttatcag attatcccga ctgttagcgg cggcctggcg caaatccagc
    10621 aacgactgat ggaagcgttg gataagatca acaaactgcc attgaatccg atgattgaac
    10681 aggcaaccag tacgctttct gaaagtcagc gcacaatgaa aaacctgcaa acgacgctgg
    10741 atagcatgaa caagatcctc gctagccagt cgatgcagca gttgccgacg gatatgcagt
    10801 caacgttgcg tgaattgaat cgcagcatgc agggcttcca gcctggctcc gcagcctaca
    10861 acaagatggt ggcggatatg cagcgccttg atcaggtgtt gcgagaactg caaccggtgc
    10921 tgaaaacgct caatgagaag agtaacgcgc tggtatttga agcgaaggac aaaaaagatc
    10981 cagagccgaa gagggcgaaa caatgaaaaa gtggctagtg acgattgcag cactgtggct
    11041 ggccggatgc agctccggcg aaattaataa aaactattac cagttacctg tggtgcagag
    11101 cggtacacaa agtaccgcca gccagggcaa tcgtctgtta tgggtagagc aggtcactgt
    11161 tcctgactat ctggcgggga atggtgtggt ttatcaaacc agtgatgtga agtatgtgat
    11221 tgccaacaac aacttgtggg ccagcccgtt ggatcaacag ttgcgcaaca ccctggttgc
    11281 caacctgagt acgcaactgc ccggctgggt ggttgcctcc cagcctctgg gaagcgtcca
    11341 ggacacgctc aatgttaccg taacggagtt taacggtcgc tatgatggca aggtcattgt
    11401 cagtggtgag tggctgttga accaccaggg acaactgatc aaacgtccgt tccgtctgga
    11461 aggagtgcaa actcaggatg gttacgatga gatggttaaa gtgctggccg gtgtctggag
    11521 tcaggaagcc gcttctattg cacaagagat aaagcgtcta ccttaattat aaagatttgt
    11581 aagtataacc gtctccggta tgttgcctga ggcggttttt ttgtttctaa cgtgcggaaa
    11641 aatttgttcc tcttcacatt ttttgtacaa ccgacatgcc cgtgtagctc acaaatatga
    11701 cagtggcgtg aattttgcgc attgacggca gttatgattc gcggtattgc ttaactgtga
    11761 ttgcacattt agtaatcact gttttctttt ccaccagaaa ccagtatgag ggaaacgagg
    11821 catgaagaga caaaaacgag atcgcctgga acgggcacat caacgtggtt atcaggccgg
    11881 catcgccgga cgctcaaaag aaatgtgtcc ctatcagacg ctgaatcaaa ggtcacaatg
    11941 gctgggaggc tggcgagaag ccatggcgga cagggtagta atggcctgat tctgtctctt
    12001 taaaaagaaa cctccgcatt gcggaggttt cgccttttga tactctgtct gatgataatc
    12061 agaaggcaga cgtatcctgg aacagaccga ctttcaggtc gctggcggta tagatcagac
    12121 gaccatcaac cagcacttcg ccatctgcca gacccataat cagacgacga ttaacaatgc
    12181 gtttaaagtg aatacggtag gtcacttttt tcgctgtcgg cagtacctga ccagtgaatt
    12241 tcacttcgcc aacgcccagc gcgcggcctt taccttcgcc gcccagccag ccgaggtaga
    12301 accctactag ctgccacatt gcgtccaggc ccaggcatcc cggcataacc ggatcgccaa
    12361 tgaagtggca tccgaagaac cacagatccg gattgatatc cagttctgct tcaacatacc
    12421 ctttgtcgaa gttaccaccc gtttcggtca ttttgaccac acggtccatc atcagcatgt
    12481 tcggtgctgg caattgcggg cctttagcgc caaacagttc accgcgacca gaggcaagaa
    12541 ggtcttcttt tgtataggat tcgcgtttat ctaccatgtt ctctgtaagc cttattttat
    12601 tgaagcacgc aggatagcta acacgtgtac gctgaacaag tccgatcagt tcggaataaa
    12661 ccagttcagc caacgtaatg gccatggaaa acggtgacgt ccttcctgtt gcgatgcttg
    12721 cgcgatacgt tcctggatgg tttgcatcag cgtcgtttgg ccttcgccat cccacaccag
    12781 atttaataat aacggcagtg cgtcagtcac atcgtctact gcccagatgg tgaatttgcc
    12841 ttcttctacc gctttcacca gttcactgtg aagacttaaa tggcgaacgt tagctgtggg
    12901 gataatgaca ccttgtttcc cggttaactc acgttgctgg caaatagcaa agaagccttc
    12961 gattttctca tttaaaccac cgaccggctg ggcgcgaccg aactgatcga ctgaacctgt
    13021 gatagcgata ctctgattca ccggcacatc ggcgagggcg cttatcaggg cgcagagttc
    13081 agccatcgag gcactatctc catcaacttc actgtatgac tgctcaaatg tcagcgatgc
    13141 tgagaagggg atctgttgct caagctgtag ttccgacatc aggaacgctt gcatgatcat
    13201 catccctttc gcatggatat tgccgccaag ttccgctttg cgttcgatgt cggtgaattc
    13261 accatcgcca atatgcacaa cgcagctaat gcgagaaggt tcgccaaaag cgcgtggatg
    13321 acccggaaat tcaatgaccg aaagggcgtt aatttgcccg atgcgttcgc cttcggtttc
    13381 aatcaggatt tgctcctgaa ggatctcatc ctgcatacgt tcagcgagga aaccttcgcg
    13441 ccattcacgc tgctgcagca ttaagtttag ctgctcgccg gagaaggtgt cgccatcaca
    13501 cagggaggcg acctctttac actggcggag gatccactgc gggctaagcg gaagtgtttc
    13561 ttgttcaccg gtgtagcgtg ctgcttcgcg gataagtacc ggccaggcat ccgctcccgg
    13621 ggcaggtaag tgattatgtc tggcggtaaa tgtcacccag cgacaccact ggcttactga
    13681 ctccgcatcg acaatctgca gagtatcttc aaattcgcta taaatagcct gctctgaaag
    13741 ctctggctcc atctcctgga aatcagccaa tgattcgcgt tcgcctacca gaatgacttt
    13801 cagcttcaat ggcatcgaag gcacagagac ggggagaggg cgcgactcat caaacgcaac
    13861 ccagtcaaaa cgctcgcggt taacgatatt tttcagccgc atccacagca gaggttgcgc
    13921 cagcagtgta cgcaaagaga tgatgagaat accgccattt gcctgatgca ccagaccagg
    13981 ctgcagggta atgtcgccat taaactggcg caggcagcca aagagttgct ccgcttctac
    14041 ccagtcggca gcgacaactt gcgttaaagt cgcaaaatta tcatctgcac tcactgcgtg
    14101 acgtaagcgg atggagtggc cggaaacctc atagtgatcg cccaccagtt ggcctgcatc
    14161 gctttgtagc gtacgcgcgg catcggcaat aagattcaga tactcagact cttccggggc
    14221 cttagccagc ataaaagagg aggatgctcg cgtatgcagc aactgctcca gcgcaaattg
    14281 cagccgcggt tgagtatcac tgaataaagg atcgttttcg tcaatcaaat gtggctgagc
    14341 aaatatttcc tgatagctat cggtatcagg aaccaggtca cgccatgcaa gtttcgtaat
    14401 ggtcaaagtt gatgtttttt agtctgttgt caaagccgcg attataccgt aaccggcact
    14461 acagcacacg tagaaagcac cgacaatact cctggcatgg gcgttaaagc tcacaggatg
    14521 gagattcttt tcttcactgg cctaaaaagc tgatattctg taaagagtta cacggtaaca
    14581 ttgagatcgc tatgaaatat caacaacttg aaaatcttga aagcggttgg aaatggaagt
    14641 atctggtgaa gaagcatcgc gaaggggagt taattacccg ttacatagaa gccagtgccg
    14701 cccaggaagc cgtcgatgtg ttgctctcgc tggaaaatga acccgtgctg gtaaatggct
    14761 ggattgacaa gcatatgaat ccggaactgg tcaatcgcat gaagcaaacc attcgggcca
    14821 gacggaagcg ccattttaat gcggaacacc agcatacgcg caaaaagtcg atcgatctgg
    14881 aatttattgt ctggcaacgt ctggctggtc ttgcgcagcg tcgcggtaaa acgctttctg
    14941 aaacgattgt tcagctgatt gaagatgcgg aaaacaaaga gaaatacgcg aataaaatgt
    15001 cttctctgaa gctggatctg caggcattgc tgggtaagga ataactgacg aaagtcagtt
    15061 caatttacta aaggcaaaaa aaaccccgca gcagcggggt ttttctacca gacgataact
    15121 taagcctgcg gctgagttac aacgtctttg atacctttaa cttcgatctc tacgcgacga
    15181 tccggagcca ggcagtcgat cagtgcagca cgctgtttca cgttgtcaca ggtgttgcca
    15241 gtaaccgggt tggattcgcc cataccacgt gcggagatct tgtctgccgg gatacctttg
    15301 gagatcaggt aatcaacaac agactgagca cggcgctcgg acagaccctg gttgtaagcg
    15361 tcagaaccga tgcggtcggt gtaacccaga acaactacgg aaccgtcttt cggatccagg
    15421 ttgctcagct ggctgtacag ctgatccaga gcagcctgac cttccggttt cagggttgct
    15481 ttgttgaagt tgaacagaac gtcagacttc agagtgaagt gcttggtctg tacttccggt
    15541 gccggagctg gagccggagc aactactgga gctgcttcgc cctgaccgaa acggtaggaa
    15601 acacccaagc tcagcatgcc gttgtccgga cgagtgccga tggtgtgtgc gtcaccgatg
    15661 ttgttggtcc actggtattc cagacgggta gcgatttcag gagtgatcgc gtactcaaca
    15721 ccgccagcga agaccggaga aacgccggtg tcgtggtttt taccataaac gttggattta
    15781 gtgtctgcac gccataccat accacccaga cgagtgtaga tgtccaggtc gtcagtgatt
    15841 gggtaaccca gtttagcggt cagttgaacg ccctgagctt tgtatgcacc gttttcaacg
    15901 ctgcctttgt acggcatacg acctaaccag tcgtaaccca tttcaaagcc aacatacggg
    15961 ttaacctggt aaccaccaaa agcaccagcg cccagttggt tttcatgggt cgggccattg
    16021 ttgttgatga aaccagtgtc atggtactgg gaccagccca gtttagcacc agtgtaccag
    16081 gtgttatctt tcggagcggc ctgcgctacg gtagcgaaac cagccagtgc cactgcaatc
    16141 gcgatagctg tctttttcat tttttgcgcc tcgttatcat ccaaaatacg ccatgaatat
    16201 ctccaacgag ataacacggt taaatccttc accgggggat ctgctcaata ttaactctac
    16261 cgatatcttc ggcttatgcc gagcacccct ggcgatgtaa agtctacaac gtagttgaaa
    16321 acttacaagt gtgaactccg tcaggcatat gaaaaaaaag tcttgtataa ggtatgttta
    16381 atcttttttg tcagcgacaa tttacagaag aaaatcgcgg aaaccgcttc agacaagcct
    16441 ccgcaaggaa aattagtcac gactgaaagc attggctggg cgacaaaaaa agttccagga
    16501 ttaatcctaa atttacttaa tgatacaaat tagagtgaat ttttagcccg gaaagttgtc
    16561 tcgtggcgtg agaggatgcg cttaccggac gcataataaa ccccatagcg ttaccttcat
    16621 ttgccgcatc aacaagttca gcatgctctt cttcagtcaa atcatctgcc aaccaaccga
    16681 tcaccacact gtaattgccc gtgcgtaaag cgcgaaccat tgactccaca gtgtggcaag
    16741 gggagagctg gctaatctgc attactttcg ttaagggtag cccagatgcc tgaacccatt
    16801 cccgactcag tttttgttgc ggtgttaacc agagttgcca gcgcgattgc tgaccgagtt
    16861 gctgtaacaa tggcaacagt agaagttgcg tcatcatggg ctgatcttcg cgatagacaa
    16921 cttcactgat aagcccggct gtagtgtttt ccgtagagac acgcgcaatt ttacttgctg
    16981 cggatgagaa cgacgaagaa cgatgtgcat agcctgaagt gtacataatc aatccagccc
    17041 ctgtgagtta ctgtatgaat gtacagtaca tccagtgaca acaaagatca accctatttt
    17101 cggaaagagc ctcgcaaatt ttttcgttgg tgacgggaaa acataaatta atcttgcccc
    17161 ttaagaataa gttgcctatt ttcgtagtta acggatccgt taatgtgaat cattctttta
    17221 tgttatgatt ttaaaaggaa ttttatgaaa agcctctcct ataagcggat ctataaatca
    17281 caagaatacc tggcaatgtt gggcacaatt gaataccgat cattgtttgg cagttacagc
    17341 ctgaccgttg acgacacggt gtttgcgatg gtttctgatg gtgagttgta tcttcgggct
    17401 tgtgagcaaa gtgcacagta ctgtgtaaaa catccgcctg tctggctgac atataaaaag
    17461 tgtggccgat ccgttaccct taattactat cgggttgatg aaagtctatg gcgaaatcaa
    17521 ctgaagctgg tgcgtctgtc gaaatattct ctcgatgcag cgctgaaaga gaaaagcacg
    17581 cgcaataccc gggaaagact gaaagatttg cccaatatgt cttttcatct ggaagcgatt
    17641 ctcggggagg tggggattaa ggatgtacgg gcgttacgta tacttggggc aaaaatgtgt
    17701 tggttgcgac tgcggcagca aaacagtctg gtgacagaaa agattctgtt tatgcttgaa
    17761 ggtgccatta tcggcattca tgaagctgcg ctcccggtgg cacgccgcca ggagcttgca
    17821 gaatgggctg actcacttac gccgaaacag gagtttcctg cggaacttga gtaatctggc
    17881 gttgcagacg accaatctca ggcagtaatg caatcaataa tccaacttgt tgtacgacca
    17941 gaggttcttt gctgtctgcc cgtggttcaa gttgctgcat ccgctgtttc aggctagcta
    18001 atgcctcatt gacgcgttct tcatcagcag gttgatgatg taacgcgtca tcaacatagc
    18061 aaactgcgtc atcaagaaac gccagaattt caggattagt taactgctcc cggtgagcac
    18121 cgagggctga gatatagctg gtaaacgtat ggttaaggca cagcaaccga aacgcggctt
    18181 cgcgaatttg cggggtaacg ttcggctcgc tggacatatt tgataccacc gacgccagct
    18241 cagcatcacg gttgtgtgca tcgcggcggg caatacgata cgccagacgg ttatcacgcc
    18301 cctgatggta ttgctccagt atggcatcga gataccgaca gttggcctct gtggcgcgtt
    18361 cgagcatgcg cggcaaattg cgaaactgcc agtcaggcca gatgtagctc actgccgccc
    18421 acgcaatggc acaaccaatc agcgtatcga ttacgcgagg taacgctact tcaaaacctt
    18481 cacccagtaa gttaaaacac agtagcacca aaagtgtgat gaacatcgtt gcatgagcgt
    18541 attgcacgtt acggaaggca aaaaagagca cgccggtaat aaccagcagc accagctgcc
    18601 cttccagtga tggcacaaac cacagcacag gaatgccaat ggcgatacct accagcgtac
    18661 caataatcct taacttcagg cggtggcgcg tggcgttata gtttggctgg cagacaaaca
    18721 aacttgtcag caagatccaa aacccgtgat gcattccggt tatttgaatg atggcgtagc
    18781 cgaagcacaa caccagcgac attcttaccg catgacggaa gagggcggat tccggcgtga
    18841 agtgacggct aagacgcagc cagatatcac tcaacccgtg cgggctgtca tcagcgagct
    18901 cattttcgtc attattatgg ggtagtgcct gggcctgttc tgattcaatt gttgccagtt
    18961 gggcatcaat ggcgcgtaaa ttgttcagca aaaatcccag tgttttgagt aaatcggcgg
    19021 gtgcgccgtt atcgcgcatc cgctccagcg cagcatcaat atgcgtaaaa gcgcgctcaa
    19081 aatgcggatc atgttgataa ggctgacgca acaaaataca gcgtgacagt tgctggcacg
    19141 cctggccctg catcgacatc agccgctgaa aacggaacag cacgtcgctg tggcgaaaat
    19201 gttcacgcaa tgtttgatac tgaatatgag aagagctggc acgctcgtga atatcctgtg
    19261 cgacaaagta ataatgcagc gtgcgacgcg ttccccgttg accacgatcg ccacgtaagc
    19321 gggtcagcag cgagagtttc gtctgattca atgtcgccat cagcagaccg ttggcgagag
    19381 ccaaatcgta cagcggtgcc tggctttcat cttcaatatc aggatcaaac atgcgcgact
    19441 tgagctcaag ataacgcgcc agttgttcat agcaacgcgc caggttgtcc tgcagcgggc
    19501 ggaccgggaa cagcagatga ccaataagtg tcaggacgtt gtaccagacg gcaccggcca
    19561 gcagatacat cggctgctga taccagtgct catacagtga tgttcccaac atagtgtaaa
    19621 tggcgatcag caatgcaccg aaggcaattg ttgcatagcg ttgacccaga ccgccgagca
    19681 aaatgaagcc gctggtagag agcgttaagc caatcgcaaa tagccagggc cagggaaaca
    19741 gcaattctac tgaggccgag gcgataaaaa agcagaacag cgtaatgatg aggttacgca
    19801 aacgtcccgc cagtcggtca tcgagatcgg tcagcgccgc tgccaccatc cccagcgtta
    19861 gcggaatcgt cagttttaca tcacccagcc accacggaaa cgctgtggtt ccacaaagcg
    19921 caataaaaat acgcgcgtaa tacagccagg cgctgttcca ggtatagcgt ttgagcaaag
    19981 gacttagcat aaaggccata ttagtggatt attcaaaacg acgacgtgca ttggcttcac
    20041 gcgcagcttg cgctgtttct accgatacca cgcgacgacc aaccggccat agtgcaatag
    20101 cggcaatttt aaagttcgca atgccgacag gaatgccaat gattgaaata cattgtgcga
    20161 tgcccgttgc aatgtgcatc aggcataacc accagccaaa gaaaatcagc cagaaaatat
    20221 tcaataccgt accgccagta ttcagcagca catttttgcc agccgggttc agttcatcga
    20281 catgaatagc ttcattgcca taaggcacca gagacagttt agtgatctcc cagcaggatc
    20341 gtgtcagcgg taaggtaaaa atcagcacaa tactgaccag agtcgccaac agccagccca
    20401 gagtggtggc aaatccgcca agcacaaagt tcagaatgtt caaaacggta cgcataaaac
    20461 ctcgctttac tgtggttttc agtaatgggc ggcaattgta acgttttttt gggctggagc
    20521 acgttttctc tgacggttac actgataaga aataatttcg tgtggatcta cagagtcatg
    20581 gaactgaaag cgacaacgct tggaaaacgt ctggcacagc acccttacga tcgggcggtg
    20641 atcctcaatg ccgggattaa agtctccggc gatcgccacg aataccttat tcctttcaat
    20701 caattactgg cgattcactg taagcgtggt ctggtatggg gcgagctgga atttgtactg
    20761 ccggacgaaa aagtggtgcg tctgcacggc accgaatggg gcgagacgca gcgtttttac
    20821 catcatcttg atgctcactg gcggcggtgg agtggcgaga tgagcgaaat tgcgtctggt
    20881 gttttacgcc agcaactgga tttgattgcc acgcgcactg gagaaaataa atggctgacg
    20941 cgtgagcaaa cctctggcgt tcagcaacaa atccgccagg ctttgtcggc gttgccgttg
    21001 ccggttaacc gactggaaga attcgataac tgccgtgagg cgtggcgtaa atgtcaggcc
    21061 tggttgaaag atattgaaag cgctcggttg cagcataacc aggcgtatac cgaagccatg
    21121 cttaccgagt atgcggattt tttccgccag gtcgagtctt caccgctgaa tccggcgcag
    21181 gcccgggcag tcgttaatgg cgagcattct ctgttagtgc tggcaggtgc aggaagcgga
    21241 aaaacgtcgg tgctggtggc ccgtgcaggc tggttgctgg cacgtggtga agcgtcccct
    21301 gagcaaattt tattgctggc gtttggtcgc aaagccgctg aagagatgga cgagcggatt
    21361 cgcgaacggc tacataccga agacattacc gcacgcacgt ttcatgcgct ggcgctgcat
    21421 attattcagc agggcagcaa aaaagttccg atagtcagca aactggaaaa tgataccgct
    21481 gcccgtcatg aactctttat tgctgagtgg cgcaagcaat gcagcgaaaa gaaagcgcag
    21541 gcgaagggct ggcggcaatg gctgacggaa gaaatgcagt ggtcagtgcc agaaggtaac
    21601 ttctgggatg atgaaaaatt acagcgtcgc cttgcctctc gcctcgatcg ctgggtaagt
    21661 ctgatgcgga tgcacggtgg tgcacaggca gaaatgattg ccagtgcacc cgaagagatt
    21721 cgcgatctgt tcagtaaacg tatcaagttg atggccccgt tattaaaagc ctggaaaggt
    21781 gcgctgaagg cagaaaacgc tgtcgatttt tcgggcctta ttcatcaggc gattgtgatt
    21841 ctggagaaag gtcgctttat cagcccgtgg aagcatattc tggttgatga atttcaggat
    21901 atctcgccgc agcgggcagc gttgttagcg gcattacgca agcaaaacag tcagacgacg
    21961 ttgttcgctg ttggtgatga ctggcaggcg atttaccgat tcagcggtgc gcaaatgtcg
    22021 ctcaccaccg ctttccatga aaactttggt gaaggcgaac gttgtgattt agacacgact
    22081 taccgtttta acagtcgtat cggtaaggtg gcaaaccgtt ttattcagca gaacccaggc
    22141 cagctgaaaa agccgctaaa cagcttaacc aatggagaca aaaaagccgt cacgttattg
    22201 gatgagagtc aacttgacgc tttgctggat aagctctctg gttatgccaa accggaagag
    22261 cgcattctga tcctggcgcg ttaccatcac atgaggcctg ccagcctgga aaaagcggca
    22321 acacgctggc cgaagttgca aatcgacttt atgaccattc atgccagcaa agggcaacag
    22381 gcggattacg tcatcatcgt tggcttgcag gagggaagtg atggttttcc ggctgcggcg
    22441 cgggagtcga ttatggaaga ggcgctactg ccaccggttg aggatttccc ggacgctgaa
    22501 gaacggcggt taatgtacgt ggcgctgacc cgggcacgcc atcgggtatg ggcactgttt
    22561 aacaaagaga atccctctcc ctttgttgaa atactgaaaa atctggatgt gccggtggcg
    22621 agaaaaccgt aagaaacagg tggcgtttgc cacctgtgca atattacttc agacggtccg
    22681 cgagataacg ctgataatcg gggatcagaa tatcgaccgc gtcgttgaaa tgcggcgact
    22741 ggattatgaa gtctgccgtt gccacgttgg tggcgaccgg aatgttccat accgtcgcca
    22801 gacgcagcaa ggctttcacg tcaggatcgt gcggcacggc atttagtgga tcccagaaga
    22861 aaatcaatac atcaattttc ccttctgaga tcaatgcgcc aacctgctgg tcacccccca
    22921 ttgggccact caacatcgcg ttgacgttca tgccggtcgc gcgggaaatt aagttaccgg
    22981 tagtgcctgt tgcatacagt acgtgttgtt ccagtaacgg ttgatgccgt tccacccagc
    23041 tcatcagcat ttgtttgcag tgatcgtgtg ccaccagcgc aatatgtttc cgcgcaggta
    23101 aagtgcgagt cgtcagttcc ataatgtaca tccgtagtca actttcctac agattactgt
    23161 aagcacttat cgctgcaaga taaagaccga aaaagcctgc gcacaggcac aaaaatctca
    23221 ggaagatggt tgtttttccg cccactgcag gaaagtattt cgcgtttgtg ggtcagccag
    23281 tttaaaccaa tacttcagcc gttgttctgt gagcacctga gactgcggtg gaatagcatc
    23341 cagctcggaa acacccgaaa gcagcgtact gtcatctgcc atcatcgtgg caaattgcgg
    23401 tagtgaagcg cgtttcgcgg atttgttata gcgttcaacc tcgacctcat aatcaatcgt
    23461 ttttgccgtt gaggtaatgg cgagaatatc cagctttacc ggaatcggcg tcgcatcgcc
    23521 atccaacaat tcaaggcgcg gcgcggcatc aaaatggtta gcttcccgct cattctccag
    23581 gcgaggcagg cgaaaattga cctggttgat gagctgggta ttaaaactga ccactagcgg
    23641 tggggagata tacagccgtt cttcactatt ggaaagatga atcgtcttct caacgcgaaa
    23701 cactaactga tgcggtccat tatccagttc aatgctgtcc gcaccgcgca gcagagagct
    23761 ggaaaccttc ttgccgtcga gtaccagaag atcgacatcg gttgaaagcc gcagcgtggt
    23821 ggcaaaaacg gataccggca aacataatgc aatcaaggtg gtcacgatgc cggttttcat
    23881 aggtagctcc tgtcaaaaga ccgcccgaat aataatgtgt caaaattttt ctttaatcat
    23941 acttactaac atatagacat atttcccgct tttgctctca ttcattcgta ttagctgcat
    24001 ggttggcatg tcggctttgt cgtacacttt gttaaacagt ctggaggaaa taatgaaaga
    24061 aaccgatatt gcaggcattt taacgtccac tcacaccatt gcgctggtgg gggcaagcga
    24121 caaacccgat cgtcccagct atcgcgtgat gaaatatcta ctcgaccagg ggtatcatgt
    24181 cattccggtt tcgccaaaag ttgccggcaa aacgctgctg ggccagcagg ggtatggcac
    24241 gctggcagac gttccggaaa aggtcgatat ggtcgatgtt ttccgtaatt cagaagcggc
    24301 gtggggcgtg gcacaggaag ctattgccat tggcgcgaaa accctctgga tgcaactggg
    24361 ggtgattaat gagcaagcgg cagtactggc acgggatgcc gggttgaatg tggtgatgga
    24421 tcgctgcccg gctattgaga tccctcgcct gggcctggcc aaataaaaaa tccccggaag
    24481 gcaaaaacct tccggggatt cgttcaggga tcagttacgc agacgcgggg cctggagttg
    24541 tttgcggatg gtctgcgcca gctcatccat ggatggctgt tcaggatgtt catcctgcaa
    24601 ttcgctactt agctgggctt cagccaggta ggtatgaacc ggtaggccgt tatcgtcctc
    24661 catcactacg tgataccacg gagcggcgcg aagctcgtca tttaccgcca gctcatcagg
    24721 cgacggttca gagagcgaat aaaccgggtc gatatccacg accactccga gataacctaa
    24781 cagggaatgg cggacctgct ggccgatacc gaatttgctg gcaatcatag tcacctcccg
    24841 ggaaatcttt acctttgata taagggtaaa attccacatt tcaagttaca tgacgcgaca
    24901 ggcaaaccct ttcagatata gcccttccgg ataggtagcg atcaccggat gatcggctgc
    24961 ctgacggaac tgctctataa attgtacatc acggccggca tcaattgcgg catccgcgat
    25021 gattttctga aataaatcgc tggtcatcag accggaacag gagaaagtca ggagaatacc
    25081 gccttcattc agcaactgaa tcgccagcat gttgatatct ttatagccac gacacgcgcc
    25141 catcaactgg cttttattct caacaaactt cggcgggtcc atcacgataa cgtcaaattt
    25201 ttcaccgcga tcgcgataag tacgcagcaa tttaaagaca tcatcacgga caaactcagc
    25261 cttgctcaga tccagtttgt tcagctcaac gttctgccgt gcaatatcca gcgcttcctg
    25321 ggaggtatca acgctgacaa cctggctgca accgcccatc agtgccgata cggcgaaacc
    25381 accggtatag gagaaacagt tcagcacacg tttattttca acgtagcggc gggtagccag
    25441 gcggctgtct cgctggtcca ggtagtagcc cgttttgtgt ccgtgctgaa tatccaccag
    25501 cagcttcatg ccgtgttctt caatcggcag cagggcaggc ggcaactcgc cggtgacgag
    25561 gcccagggtc agctccatcc cttctttttt acgtaccgca acatcgctgc gatcgtaaat
    25621 cgcacattcc gggtacagcg tttgcagggc actaactaat gccgcgcgct gatattctgc
    25681 cccagcactc agcagttgca gcaccagaaa attaccgaaa cgatcgatag taatacccgg
    25741 caggccatca gattctccgg cgattaaacg atagctgtcg aggccatctt tttgcgccag
    25801 ccagtcacgc catttttgtg cttgttgcaa acggcgggaa aaaaaagcaa tgtcgataga
    25861 ctcagacggg tcaaacgtcc agacgcgcgc ccggatttgc gaagctggcg aataagcgcc
    25921 gcgtgctaac cattttccct gatgatcaac aatatcgatg gtttcaccga ggctggcttt
    25981 accttccatg cgggcaacgg ccccggaaaa gacccacgga tggcgacgaa gtaatgattt
    26041 ttcgcgccct ttggctaaca ctaaacgtac actcataatt tacttttctg tcgatgcaaa
    26101 gaaatgggcg tcattgtccg gagttttcca tggatttgca acgcgtccat taaggataag
    26161 gagagcagca atgtcgaaag tctgcataat tgcctgggtt tacgggcgag ttcagggcgt
    26221 aggatttcgc tacaccacac agtacgaagc gaaaagactg ggtttaaccg ggtacgccaa
    26281 aaatcttgac gatggcagcg tggaagtggt tgcctgcggt gaagaagggc aggtggaaaa
    26341 attaatgcag tggttaaaga gtggcggccc gcgttctgcg cgtgtagaac gggtgctcag
    26401 cgagccgcat catccctcgg gggaattaac cgattttcgt atccgttaaa tacattttac
    26461 cggttttggc aggccagcaa ttttggtggc ttgcttagcc ggacctttcg gaaacagtcg
    26521 gtataaatag cggctgttac ctttttcttc gccaaattta ttcgccatcg cttttaccag
    26581 catacgaatc gccggagaag tattgaattc cagatagaaa tcacgcacaa aacgcaccac
    26641 ttcccagtgt tccggtgaca gcgaaatccc ttcgttctct gcaatcacca ctgccagcgg
    26701 ctcactccac tggctgcttt ctttgagata gccttcggta tccgtttcta tctctttacc
    26761 ttcgaagatc agcataatta ttactacctt aatcagactg gcggcagtgt aacaaaaaac
    26821 aaagccccgc ataaagcgag gctatgaaag tgttagcggg tgagattaat cgcggctggc
    26881 gaagcccaga atgctcagca ggctgacgaa gatgttgtac agcgaaacat acaggctaac
    26941 cgtggcacga atatagttcg tttcaccgcc atgaacgatg ttgctggttt caaacaaaat
    27001 agcgccagag gagatcaaaa tgaagaccgc actgatcgcc agatgcagag caggcagctg
    27061 caggaagata ttagcaacca taccaatcag caccaccaca atacccgcca tcagcatacc
    27121 gccgaggaac gacatatctt tgcgggtggt cagcacatat gcagagcagc agaagaacac
    27181 taacgccgtt ccgcccagag ccatagcgat tacgtccccc attccggcag acagataggt
    27241 gttcagaata ggtccgagga tataacccag aaaaccggta aaggcgaatg cggagataat
    27301 cccggtcggc ttattcgccg ttttataggt caggaacatc aaaccataca tacccaccag
    27361 cgtcagaatc agacccggag atggcagcat cagcaccgta ctggcagttg cggtaatcgc
    27421 cgaaaaggcc agcgtcaggc tcagcagaaa ataggtatta cgcagcacct tatgggtgct
    27481 aagcagtgat gtacggtcat gtgaagaact aacaatacga tccattgagt cactctctat
    27541 gacagatgta attaattaag cagcataatg ataatgcgta agggcaccca gaagttttac
    27601 ccatctttac gcatttgatc tggaacaggt ttaacagcgg attatcaggt cattaagcaa
    27661 atataacgcc ctgagaattt cgacaggcaa aagaaaaagg ggttagcatt tagctaaccc
    27721 cttatcttat ttggcggaag cgcagagatt cgaactctgg aaccctttcg ggtcgccggt
    27781 tttcaagacc ggtgccttca accgctcggc cacacttccg gaatgacgcg cactataaac
    27841 atcccgatgc ggcgtgtaaa cccctaattt gtttgtttgc ctgaaaaaca gccaaaagtg
    27901 cattgatagc gtgaaataac agcagattga tcatttcatc accatgaatt ccttctcttt
    27961 tactcgttta gcaaccggct aaacatcccc accgcccggc aaaaagaaaa ataggtccat
    28021 ttttatcgct aaaagataaa tccacacagt ttgtattgtt ttgtgcaaaa gtttcactac
    28081 gctttattaa caatactttc tggcgacgtg cgccagtgca gaaggatgag ctttcgtttt
    28141 cagcatctca cgtgaagcga tggtttgcct tgctacaggg acgtcgcttg ccgaccataa
    28201 gcgcccggtg tcctgccggt gtcgcaagga gaagagacgt gcgatatgaa taacgaggaa
    28261 acattttacc aggccatgcg gcgtcagggc gttacccggc gcagctttct caaatattgt
    28321 agtctggctg ccacgtcgct gggattaggc gcgggaatgg caccaaagat tgcctgggcg
    28381 ctggagaaca aaccccgcat tccggtggta tggatccacg gtctggaatg cacctgctgt
    28441 accgaatctt ttatccgctc cgctcaccca ctggcgaagg acgtcatcct ttccctgatt
    28501 tccctcgatt atgacgatac tttgatggct gccgccggaa cccaggcgga agaagtcttc
    28561 gaagacatca tcacgcaata caatggcaaa tatatcctcg cagtagaagg taatccgccg
    28621 ctgggcgagc aggggatgtt ctgtatcagc agcggtcgac cgtttattga gaaactcaaa
    28681 cgtgccgctg ccggagccag cgcgattatc gcctggggaa cctgcgcgtc ctggggctgc
    28741 gtgcaggccg cgcggcccaa tccgacgcag gcaacgccta tcgacaaagt catcaccgac
    28801 aaacccatta tcaaagtacc tggctgcccg ccgatcccgg atgtgatgag cgccatcatt
    28861 acttacatgg tgacctttga tcgcttgcca gatgtcgaca gaatgggccg tccgctgatg
    28921 ttctatggtc agcgaatcca cgataaatgc tatcgccgcg cccacttcga cgccggagag
    28981 ttcgtccaga gttgggatga tgacgctgcc cgcaaaggtt actgcctgta caaaatgggc
    29041 tgcaaagggc ctaccaccta taacgcctgt tcctccacac gctggaatga tggcgtttct
    29101 ttcccaatcc agtctggtca cggctgcctg ggctgtgcgg aaaatggttt ctgggatcgc
    29161 ggttcgttct acagccgcgt ggtcgatatt ccgcaaatgg gtactcattc caccgccgat
    29221 accgtcggtt taaccgcgct tggcgtggtg gcagcggctg ttggtgtgca cgcagtcgcc
    29281 agcgccgttg accagcgcag acgtcataac cagcaaccta cagaaaccga acatcagcca
    29341 ggcaatgagg ataaacaggc atgagcactc agtacgaaac tcagggatac accatcaata
    29401 atgccggacg ccgcctggtg gtcgacccga ttacgcgcat cgaaggccac atgcgctgcg
    29461 aagtgaatat taacgatcag aatgtgatca ccaatgccgt ctcctgcggc accatgtttc
    29521 gcgggctgga gatcatccta caagggcgcg acccgcgcga tgcgtgggcg ttcgttgaac
    29581 gtatctgcgg cgtctgtact ggcgtacacg ccctggcttc ggtttacgcc atcgaagatg
    29641 ctatcggtat taaagtgccg gacaacgcca atatcatccg caacattatg ctggcaacgc
    29701 tctggtgcca cgatcatctg gtgcacttct atcagcttgc cgggatggac tggatcgatg
    29761 tgttagatgc gctgaaagcc gacccgcgga aaacctccga actggcgcaa agtctctcct
    29821 cttggccgaa atcatcccct ggctatttct tcgacgtaca aaaccgcctg aaaaaatttg
    29881 ttgaaggcgg gcagttgggg atcttccgca atggctactg ggggcacccg cagtacaaac
    29941 tgccgccaga agctaacctg atgggctttg cccactatct cgaagctctc gatttccagc
    30001 gtgaaattgt caaaatccac gcggtctttg gcggtaaaaa cccgcatcca aactggattg
    30061 tcggcgggat gccttgcgcc atcaacattg acgaaagcgg cgcggtcggg gcagtcaata
    30121 tggaacgcct gaacctggtg cagtcaatta tcacccgcac ggcggacttc attaacaacg
    30181 tgatgatccc cgacgcctta gccatcggtc agttcaacaa accgtggagc gaaatcggca
    30241 ctggtctttc tgataaatgc gttctcagct acggcgcatt cccggatatt gccaacgact
    30301 ttggcgagaa aagtctgctg atgcctggcg gcgcggtgat taacggcgac ttcaacaatg
    30361 tgctgccagt ggatttggtt gatccgcagc aggtgcagga gtttgtcgac cacgcctggt
    30421 atcgatatcc caacgatcag gtcgggcgtc atccgttcga tggcatcacc gacccgtggt
    30481 acaaccccgg cgatgtcaaa ggcagcgata ccaacattca gcagctgaat gaacaggaac
    30541 gctactcgtg gatcaaagcg ccacgctggc gcggtaacgc gatggaagtg gggccgctgg
    30601 cgcgcacgtt aatcgcttat cacaaaggcg atgctgcgac cgttgagtcg gtcgatcgca
    30661 tgatgtcggc gttgaacctg ccgctttccg gtatccagtc aacgttaggc cgcattttgt
    30721 gccgcgcgca cgaagcgcag tgggccgcag gtaagttgca gtatttcttc gacaagctga
    30781 tgaccaacct gaaaaacggc aatctcgcca ctgcttccac ggaaaaatgg gaacctgcaa
    30841 cctggccgac agagtgccgt ggtgtcggtt ttaccgaagc gccgcgcggg gcgttaggcc
    30901 actgggccgc cattcgcgat ggcaagattg atctctacca gtgcgtggtg ccgaccacct
    30961 ggaacgccag cccgcgcgat cccaaagggc agattggcgc ttatgaagcg gcgctgatga
    31021 acaccaaaat ggcgatcccc gagcaaccgc tggagatcct gcgtactctg cacagctttg
    31081 acccgtgcct cgcctgttca acacacgtgc tgggcgacga cggtagcgag ctgatctccg
    31141 tgcaggtgcg ttaacagcga aggagaatca tcatgcaaca gaaaagcgac aacgttgtca
    31201 gccactatgt ctttgaagcg ccagtccgca tctggcactg gttgacggtg ttatgcatgg
    31261 cggtgttgat ggtcaccgga tactttatcg gcaagccgct accttccgtc agcggcgagg
    31321 cgacgtatct gttctatatg ggctacatca ggttaattca cttcagcgcc gggatggttt
    31381 ttaccgtggt tttgctgatg cggatctact gggcttttgt tggcaatcga tactcccgcg
    31441 agctgtttat cgtgccggta tggcgtaaaa gctggtggca gggcgtgtgg tatgaaatcc
    31501 gctggtatct gtttctggca aaacgtccga gtgccgatat aggccataat cccatcgccc
    31561 aggcggcgat gttcggctat ttcctgatgt cggtctttat gatcatcact ggttttgcgc
    31621 tgtacagcga acacagccag tacgctattt ttgcgccgtt ccgttatgtg gtggaatttt
    31681 tctactggac gggtggcaac tcaatggaca ttcacagctg gcatcggctg gggatgtggc
    31741 tgattggcgc gtttgtgatc ggtcatgtct acatggcgct gcgtgaagac atcatgtccg
    31801 acggcacggt gatctccacc atggtcaacg gctaccgtag ccacaaattt ggcaaaataa
    31861 gtaacaagga gtgttcatga gcgagcaacg cgtggtggtc atggggctgg gcaacctgct
    31921 gtgggccgat gaaggcttcg gcgtgcgggt ggcggaacgg ctgtatgccc attaccactg
    31981 gcccgagtat gtggagattg tcgatggcgg tactcaggga ctgaacttgc tggggtatgt
    32041 cgaaagcgcc agccatctgt tgattctcga tgccattgac tacgggctgg aacctggaac
    32101 gctgcgaacc tatgccggag aacgcattcc ggcttatctc agcgcgaaga aaatgagcct
    32161 gcatcagaac agtttctccg aagtgctggc gctggcagat atccgcggac atctgccagc
    32221 acatattgcc ctcgtcgggc tgcaacccgc gatgctcgac gactacggcg gcagcctgag
    32281 cgaactggca cgggagcaac tgctcgctgc ggaacaatcg gcgctggcgc agttggctgc
    32341 gtggggaagt gtgccgcaac tggctaatga atcgcgttgt ctcaattatg actgtctgtc
    32401 gatggaaaat tacgaaggcg ttcgcttgcg ccagtaccgg atgacacagg aggagcaggg
    32461 atgagcaacg acacgccatt tgatgcgttg tggcaacgaa tgctggcgcg cggctggacg
    32521 ccggtcagtg aatcccgtct tgacgactgg cttacgcaag cgccagacgg cgtggtgtta
    32581 ttaagcagtg acccgaaacg cacgccagag gtcagcgata atccggtaat gattggcgaa
    32641 ttactgcacg agtttcccga ctatacatgg caggtggcga ttgctgacct tgagcagagc
    32701 gaagccatcg gcgatcgctt tggcgtcttt cgctttccta ccactttagt gtttaccggc
    32761 ggaaactatc gcggcgtgct gaatggtatt cacccgtggg cggaactgat aaacctgatg
    32821 cgcgggcttg tcgaaccgca gcaggagcgt gcctcatgag cgaaactttt ttccatctgc
    32881 tggggccagg aacgcaaccg aacgatgaca gtttcagcat gaatccactg ccgatcacct
    32941 gtcaggtgaa tgatgaaccg agtatggcgg ccctggagca atgtgctcac agcccgcagg
    33001 tgattgcgct gttaaacgag ttacaacatc aactaagcga acgccagccg ccgttgggcg
    33061 aggtgctggc agtcgatctg ttaaatctca acgccgacga tcgtcacttt atcaatacgc
    33121 ttctcgggga aggggaagtg tcagtgcgca ttcagcaggc tgacgacagt aaaagtgaaa
    33181 tacaggaggc gatcttctgc ggattatggc gggtgcgcag acgtcgcggc gaaaagttgc
    33241 tggaggacaa actggaggct ggctgcgcgc cgctggcgtt gtggcaggcg gcaacgcaaa
    33301 atctcttgcc gacagattcg ctgttaccgc cgcccattga tggcctgatg aatggcctac
    33361 cgttggcgca tgagttactg gcacatgtac gtaaccccga cgcgcagccg cacagcatta
    33421 atctgacgca attacccatc agcgaggctg atcggctttt tctctcacgt ctctgtgggc
    33481 cgggaaatat tcagattcgt accattggct atggcgagag ctatatcaac gccacggggt
    33541 tacgccatgt ctggcattta cgctgtacgg acaccttaaa aggcccgtta ctggaaagtt
    33601 atgaaatctg cccaataccg gaagtggtgc tggcagcgcc agaagatttg gtcgactctg
    33661 cgcagcggct tagcgaggta tgtcagtggc tggcggaagc tgcaccgacg taaaaagacg
    33721 gtaagtatcg ctttcagtct tatgaatatc gcaatcggcg aatacctctg gtcgtagagt
    33781 ttcaggataa agagggagat ctaacattat cgggttattt ttctctcttc gcctacagga
    33841 gtgcgcatgt gggatgtcat tgatttatcg cgctggcagt ttgctctgac cgcgctgtat
    33901 cactttttat ttgtacccct taccctgggg ctgatttttt tgctggctat catggaaacc
    33961 atttacgtgg tcaccggcaa aacaatctac cgcgatatga cgcgcttctg gggtaagctc
    34021 ttcggtatca attttgctct tggcgtggct accggcctga ccatggagtt tcagtttggt
    34081 actaactggg cattctattc caactatgtg ggcgatattt ttggcgcacc gctggcgatg
    34141 gaagcattaa tggccttctt cctcgaatcc acctttgtcg ggcttttctt cttcggctgg
    34201 cagcggctga ataaatacca gcacctgctg gtgacgtggc tggtggcgtt cggttcaaat
    34261 ctctctgcat tgtggatatt gaatgccaac ggttggatgc aatacccgac cggtgcgcat
    34321 tttgatatcg acaccctacg tatggagatg accagcttca gcgagctggt ctttaacccg
    34381 gtcagccagg tgaaatttgt gcacaccgta atggcgggct acgtgaccgg ggccatgttt
    34441 attatggcga tcagcgcctg gtatttactg cgcggacggg agcgcgatgt tgcattacgc
    34501 tcgtttgcca tcggttccgt cttcggtact ctggcgatta tcggcacgct acaactcggg
    34561 gacagttctg cgtatgaagt cgcgcaagta caaccggtaa aactggcggc gatggaaggg
    34621 gagtggcaaa cggaacctgc tcctgcaccg ttccatgtgg ttgcctggcc ggaacaggat
    34681 caagagcgta acgcctttgc catcaaaatt cccgcgctgt tagggatcct cgccactcac
    34741 tcattagata aacccgtgcc gggtctgaag aatttgatgg ctgaaaccta cccacgcttg
    34801 caacgcggac gtatggcctg gctgttaatg caggaaatat cgcaaggcaa tcgtgagccg
    34861 catgtgttgc aggcattccg ggaactggaa ggtgacctgg gctacggcat gttgctctcc
    34921 cgctatgcgc cggatatgaa tcatgtcaca gccgcacagt acctggcggc gatgcgtggc
    34981 gcgatacctc aggttgcgcc ggtattctgg agtttccgca tcatggtggg ctgtggttcc
    35041 ctgctgctac tggtgatgct gattgcgctt gtccagacgc tgcgtggcaa aatcgaccag
    35101 catcgctggg tgctgaaaat ggcgctctgg agtttgccgt tgccgtggat tgcgattgaa
    35161 gccgggtggt ttatgaccga gtttggtcgt cagccgtggg cgatacagga catcttaccg
    35221 acatactccg cgcactccgc tttaaccaca ggacaactgg ctttctcact gatcatgatc
    35281 gtagggcttt acaccctgtt cttaatcgcc gaagtctacc tgatgcagaa atatgcccgt
    35341 ctggggccga gcgcgatgca gagtgaacaa ccgacgcagc aacaggggta aagaagaaaa
    35401 tcatgtttga ttatgaaaca ttgcgcttca tctggtggct gctgattggc gtgatcctgg
    35461 tggtctttat gatctccgac ggatttgaca tggggatcgg ctgtctgctg ccgctggtgg
    35521 cgcgtaatga tgatgaacgc cggatagtga taaacagcgt tggtgcacac tgggaaggca
    35581 accaggtctg gttgatcctc gctggtgggg cattatttgc cgcctggccc agagtgtatg
    35641 cagcggcgtt ttccggcttt tatgtggcga tgatcctggt gctgtgctca ctgttcttcc
    35701 gcccgctggc ctttgattat cgcggaaaaa tcgccgatgc acgctggcgt aaaatgtggg
    35761 acgccggtct ggtcatcggc agtctggtgc cgccggtagt cttcggtatc gccttcggca
    35821 acttgttgct cggcgtgccg tttgccttca caccgcaatt acgcgtggag tatctcggca
    35881 gcttctggca actgctgacg ccattccctt tattgtgcgg attgctcagc cttgggatgg
    35941 taattttgca aggtggcgtc tggttacaac tgaaaactgt tggtgtgatt catctgagtt
    36001 cacagctggc caccaaacgc gctgcactgt tggtgatgct gtgctttttg ctggcgggtt
    36061 actggctgtg ggtcggtatt gatggctttg tactgctcgc ccaggatgct aacggtcctt
    36121 ccaatccgtt aatgaaactg gtggcagtgc tacctggtgc ctggatgaat aatttcgtcg
    36181 aatcgcccgt tttgtggatc ttcccgctgc tggggttctt ctgcccattg ctgacggtga
    36241 tggcgattta tcgtggtcgc ccaggttggg gatttttaat ggcgtcattg atgcaatttg
    36301 gcgtgatttt cacggcaggc atcacgctgt tccccttcgt catgccgtca agcgtgagtc
    36361 cgatctccag cctgacgttg tgggacagca cttccagcca gctgacgctg agcattatgt
    36421 tggtaatcgt gctgatattt ttgcccattg tgttgctcta cactctctgg agctactaca
    36481 aaatgtgggg gcgcatgaca acagaaactc tccgccgtaa cgaaaacgag ttgtactaag
    36541 gagcaggaac aatgtggtat ttactttggt tcgtcggcat tttgttgatg tgttcgctct
    36601 ccacccttgt gttggtatgg ctggacccgc gtctgaaaag ttaacgaacg taggcctgat
    36661 gcggcgcatt agcatcgcat caggcaatca ataatgtcag atatgaaaag cggaaacata
    36721 tcgatgaaag cgatcttaat cccattttta tctcttctga ttccgttaac ctcgcaatct
    36781 gcattcgctc agagtgagcc ggagctgaag ctggaaagtg tggtgattgt cagtcgtcat
    36841 ggtgtgcgtg ctccaaccaa ggccacgcaa ctgatgcagg atgtcacccc agacgcatgg
    36901 ccaacctggc cggtaaaact gggttggctg acaccgcgcg gtggtgagct aatcgcctat
    36961 ttcggacatt accaacgcca gcgtctggtg gccgacggat tgctggcaaa aaagggctgt
    37021 ccgcagcctg gtcaggtcgc gattattgct gatgtcgacg agcgtacccg taaaacaggc
    37081 gaagccttcg ccgccgggct ggcacctgac tgtgcaataa ccgtacatac ccaggcagat
    37141 acgtccagtc ccgatccgtt atttaatcct ctaaaaactg gcgtttgcca actggataat
    37201 gcgaacgtga ctgacgcgat cctcagcagg gcaggagggt caattgctga ctttaccggg
    37261 catcggcaaa cggcgtttcg cgaactggaa cgggtgctta attttccgca atcaaacttg
    37321 tgccttaaac gtgagaaaca ggacgaaagc tgttcattaa cgcaggcatt accatcggaa
    37381 ctcaaggtga gcgccgacaa tgtctcatta accggtgcgg taagcctcgc atcaatgctg
    37441 acggagatat ttctcctgca acaagcacag ggaatgccag agccggggtg gggaaggatc
    37501 accgattcac accagtggaa caccttgcta agtttgcata acgcgcaatt ttatttgcta
    37561 caacgcacgc cagaggttgc ccgcagccgc gccaccccgt tattggattt gatcatggca
    37621 gcgttgacgc cccatccacc gcaaaaacag gcgtatggtg tgacattacc cacttcagta
    37681 ctgtttattg ccggacacga tactaatctg gcaaatctcg gcggcgcact ggagctcaac
    37741 tggacgcttc ccggtcagcc ggataacacg ccgccaggtg gtgaactggt gtttgaacgc
    37801 tggcgtcggc taagcgataa cagccagtgg attcaggttt cgctggtctt ccagacttta
    37861 cagcagatgc gtgataaaac gccgctgtca ttaaatacgc cgcccggaga ggtgaaactg
    37921 accctggcag gatgtgaaga gcgaaatgcg cagggcatgt gttcgttggc aggttttacg
    37981 caaatcgtga atgaagcacg cataccggcg tgcagtttgt aatgcataaa aaagagcatt
    38041 cagttacctg aatgctctga ggctgatgac aaacgaagaa ctgtctaatg cgtagaccgg
    38101 aaaaggcgtt cacgccgcat ccggccactt tcagttttac tctttctcgg agtaactata
    38161 accgtaatag ttatagccgt aactgtaagc ggtgctggcg cgtttaatca caccattgag
    38221 gatagcgcct ttaatattga cgcctgcctg ttccagacgc tgcattgaca aactcacctc
    38281 tttggcggtg ttcaagccaa aacgcgcaac cagcaggctg gtgccaacag aacgccccac
    38341 gaccgcggca tcactcaccg ccagcatcgg cggcgtatcg acaatcacca gatcgtaatg
    38401 gtcgttcgcc cattccagta attgacgcat ccgatcgcgc atcagcagtt cagacgggtt
    38461 aggtggcacc tgaccgcgag taatcacatc aaagcctcct ttgccaaaat gctggatcac
    38521 tttgttgagc tcatctttac ctgccagata ttccgacaag ccatgttcat tactcacggt
    38581 aaacaggtta tgcgaataac cacggcgtaa gtcggcatca ataaataaca ctttttgatc
    38641 ggactgggcg atcaccgctg ccagagttga actgacaaac gttttaccac tgtctggcgt
    38701 cgcaccggtg atcatcagaa tgttattctc cgtctccatc atagcgaagt gcagactggt
    38761 tcgtagcgca cgtacggctt ccacagcaga atccgccggg ttatccaccg ccaggaaggg
    38821 gatattttta gtacgatggc gctgctgatt agaaaataaa tttttcttac gcagacgggt
    38881 acgtttatcc agccactcgg acattgggat agtggcataa acgctgatgc cgtgctcttc
    38941 cagttgttcc ggggcttcta caccacgacg caacatcgca cgcgccagca cggcacccac
    39001 cgaaataaac aggccaagaa taaaaccaag caccacgttc aacgcttttt tcggtttcac
    39061 tggctgcggc tgagtgactg ctgggtcgat aatccgcacg ttaccaatgg cactggattt
    39121 cgaaatactc aactcctgct ggcggttaag taattgcaga tataccgcac ggcccgcttc
    39181 tacgtcacgg cttaaacgta acacttcctg ttgagtggaa ggcattgccg atacccgctt
    39241 attcaggcgt ttgcgttctt gctccagcgt ctggcgtttt tccagcagcg cacgataagt
    39301 tgggtgatct ttcttataca gctgggagat ctctgcctcg cggaaagtca gctcattgag
    39361 ttgattatca acgttcacaa tctgctcaag aacggctttg gcttctaggt taaggtcaac
    39421 cgaatcgcgc tgctggcgat aaacgttgag tttttcttcc gcctggtcca gctcgctgcg
    39481 cacttcaggc aactggcgct gtaagaattc aaggctttgt gaatcctgcg ccgcctggcg
    39541 agcgatattc tgttgcaaat agttgttagc gatgctgttc agaatacgag taatcaactg
    39601 gggatcatca ccagtcatgg taagttccag catcccgctt tctttactgc gttcgctaac
    39661 ggtaaaggtt tcctgcaatg cgttaatcgc ttccagttcg gtacgctggc tcaggacaaa
    39721 ctgtgttcct ggtttggcct taatgtccgc gatagtcagc gcaacgccat ctttttccag
    39781 acgctggccg accataccat tgacggtgaa ctcttcacct tccagtgtat agtggccgtt
    39841 ttccccaacc gtgagtgtca gttgctgatc ctgaccattc agttgtggaa tatgcatcca
    39901 gctgatcgcc agctcacctg gtttttcttt ggttaatctc gcccagccgc gacccacaat
    39961 cggaaaatac ttctgctcaa ctatgtcgcg cagattcagt tcagcaatgg ttttaccgag
    40021 aatcatgcgc gattgcagca gttggatctc cggtgcagac tcgggcgatg agttagggat
    40081 catatcgctc aggccgctga gaatggcgtt gccctgtttt tgctcaacct ggaccagagt
    40141 atctgcctga taaattggtg tgcttaacag cgagtaagcg acagcgatca gcgtgaataa
    40201 cgcggttacg ctgataataa acttacggtg atcccataac tcgccgacca gacgaagcag
    40261 atcgatctca ttttcctgag tgctgcctgg tggcgtattc atatttttag ttgtcatcgt
    40321 attttataaa ctcaaattac cggctgaggc gcttcgccca ttcctgactg gcgcgctcca
    40381 acataccgta gacatgttca aatgcgtcct gacttttacg atagggatcc gggatctctt
    40441 tctgttccag ccattgccca aacagcattg tcttcccgcg gacctcggga gcgattgcgg
    40501 taacctgagc aatatgctct gactccattg ccagaatcag atcgtaattt cgtgccatct
    40561 cagcagtgag cttacgtccg gcatgtcctt caagagatac gccatgatta gcggcaacat
    40621 ctgctgctgt cgcatctgca gggtgtttta ccagaccatg aacacctgcc gatttgactt
    40681 ttacgcctgg cagacgttta cgcagcaagc gctcgccaat gggcgaacgg caaatgttgc
    40741 cggtacaaac caccaggatg gagttaaatt ttagttgggc catgtttgaa tccaccgtga
    40801 agtctctgtc aggtcatgca ccccggaaat cgtcggtacc agcagagaaa tcacacggtt
    40861 ccaacgagcc agcggcgcgg tagtgacata aacaatgtcg taaggttcaa gctggaattc
    40921 cgttccgagt atcattgccg atgcgtcctt cgcattcagc tgataaatat tggcgatttt
    40981 gccattctgc ttgttttgcg ttgcacgaat aacaaaaatc cccgtcgcat cagcgacatc
    41041 ctgattcata ccctcagcgt tacccagcgc ttccgccagg gtcataccgc tgcggtccat
    41101 tttcagcgtg ctctgtttac ccacttcacc catcacgaag actttcagat catcgttgcg
    41161 cggaataaat aaaatatcgc ccgggtgcag cagtttgttc tggcgcaagt cgccacgctg
    41221 catcagcgca tagaggttca ctttggtctt tacgccattc tgggtgagca ccacgttgcg
    41281 ccagtctgca tctgcggtca gaccgcccgc ggcgttaatc gcatccatga tggtcagagg
    41341 aatattggta atcggctgct gacccgattt gctgacttcg ccggtgacgt acgctttttg
    41401 cgatcggaac gccgcgacgc tgacatcgac ttgcgggctt tcaatgactg aatcgagacg
    41461 cgctgtaatc tcgttgcgta cttgggtaag tgttttcccg gccactttca ggcgaccaat
    41521 ataggggtag aaaatcgcac catcggcgtt aacccagttg ccggtatcac tggcgctacg
    41581 gtattgccct gcgggggtgg ttagttccgg gtgatcccag accgtaacca tcaacacatc
    41641 accaatcccg atacgatatt catagtttgc gatttgctgc tccagctccg ggttagcctg
    41701 cgacataatc ggtttggcgc gcaactgatc aatcaatcct ggcgtgacgg gatagatatt
    41761 gaccattttg tccaggtcat actgattgtc aggtagttca atgacatcct tgttcctggt
    41821 cgataaattc tgaccaggaa caagggtaca tgctgtcaaa gacagaacag ccaatgtcag
    41881 taccgaaaac ttaaaaatgt tctttttcat tggtttatat ttatcagtca atgtaatgtt
    41941 atgaattacg tcaggttatt gtccaaccgg tacttctcgc tctgccgtca ttggatagag
    42001 ctggtatttg cgtgacagca tttgtccacc atcacgcgtc aatggtgtcc acgaaaccac
    42061 cgcgcggttg cggttaggtc caatggtcat caagtcgaat ggaatcgaga tataaaaacc
    42121 tttactaaag ccgccttcgc cgtagtcatc tttagatacg ttactgattg ccgcccatac
    42181 ccctaccgcc acgccgctgt caaagcgttt ggcgacgtcg atcgttgctc ctttatcttt
    42241 tgccagatat tgcccaacgc taagtttcat cagtacgcca ttgagcgtcg gcgggttcca
    42301 gtaagccgtc acgaaaccag ttggcgtgga ataatcggtg aagcgcatca tgttgtccca
    42361 gtcacgttgc ttcacgtagt taacgtccac acccagcgcc cagctggcat ctagcgggcg
    42421 ataaagcagc tcggaaccga caccggcgta catcgtttcc agataaccgc catacacctg
    42481 gccatagaaa ccattgccta agtcggcaaa gtagttcgcc tgcaagttgt tgagataaac
    42541 gtcattgcga acgtaatcac ggatatgcgt gcgcacgcgg ggcagggtag agtccgcggg
    42601 caacagcgaa gacttaaact tgtcgtagtt gttgtaaata ttggtgaaaa taccgccatc
    42661 aagcagcagg tggtcggtaa accagtagcg ggcactggac atcagcccca gctggaacat
    42721 atagaaatct tccgggccgc ccagcgactg tgaaagtgtt gggttgaaag agtagctaaa
    42781 gcgatcttca cgaatacggt agccccgacc aaaggcagaa agatcttctg cttcaacacg
    42841 ttgttgttga agttgctctg attgaccagg cgctgtacca gccagctgtt tgcgcaggct
    42901 ggctacgtcg gtttcggtag tcaccatcgc catatgctcg cgcttttgcg tcacgctaat
    42961 cttctcaacg ccttgcggca ggttattcac cagaatccgg ttggcacgat cgaccgcttc
    43021 gcgagaatca cggtatttgt attgttgacc agacatatac agcgtcttat cgcgcagctg
    43081 aatttccggt gcttcaaaac ctgcgttgta cttcagcgcg gtaagttggt tcgctactgt
    43141 ggtgtactgc aatccttcag attcaggcgc aggttgatat gccggttttg gcgtatcgcg
    43201 cagggcaggg cgcagatcgt tgaaattggt ccgtaacgtg aagccaaaca tcaacgtgtt
    43261 accgcgttca taactcaggt tgagatctgc ccagctggca gcgcgataaa ctgcgccgac
    43321 gttgaaatgg cttgcctgag gcagtttgcc agcgaaatca ttctggtaat tgttgccatc
    43381 gtattcgagt ttcagacgca ggggattcca cggcgtttga tactcaatgc cgccaaagat
    43441 ggaagccggg ccacgaaaga tatcgctaaa gctgatatcg cccgcatcgt gagactctgc
    43501 gcgatgacaa tatttatcgt tcacccggca aaacgggttg gtaatattgc ccgcattacc
    43561 ggcgtagccc catgccatcc cgagggtgaa atcaaatggc cccgcttgct tgctggccac
    43621 cagatactca ccgtcaaaca gacccgtacc agcaatatca cgtttaccaa acgccacttg
    43681 cggtagccag taaccttctt cccacaggcg aagtttaaaa tcgaatgatt tgtctttgta
    43741 tgactgatcg ccgctgaaat cttcccactg gctatatttg cgtgtgcgca catccgtata
    43801 acgaatggtg ccttccagcc atgggaacag cgccacggag gtggagtaga accggtattg
    43861 atcgttatcc cggtagttga cgctgaattc accttccggt gcgatgcgcg catttggcat
    43921 ctgcaacaat cctgtgccgc caaagtctga ttgcgacgaa cccagcggat ccgggtaggt
    43981 taatacttca gcatgacagg cactggagac ggcaatggcc aggcagctta aaagataaga
    44041 attcttcttc atcagtcagg aacacgttgc gttaaaacag acacaatctg gtcatttaaa
    44101 tcagcatatt tttcgggcag tacatgcgca gaaaagccca accaaagctg gctgccaggc
    44161 ggtgggtcca catgtctttt attccacagg gcaacagggg cgacgacagt ctcaccctca
    44221 ggcgtaatca ccataacgtt attcttgtcc gcgcctgcaa ggcgggggtg atcttgcaga
    44281 taatcggtaa cgctacggcc tgcctgccag ggcaattgcc cggccccaga aaccgcaccc
    44341 agcagagtaa tagtcaccgg acgctgtacg gtatagagcg tatagtcccc gaccaacggg
    44401 ggattgctgt tctcatccac tcgaacaaaa tcagggtcca gtttgaccgg taagcgacca
    44461 gtgatattga gattgagtaa ctgctggcgc acagatttaa tcgttgccgc cacatcgtcg
    44521 tcagcttctg cttcccagga agcaagctgt gccatgacat gttggtagtc tttaagcgct
    44581 ttagctttcg ccgcagagtc ggtcagcaag gcaccaggcc accagaggcg atcgcgcagt
    44641 tgcggttgtg tcaccaactg gacaacattt tccatggggc caacggaaag tgtctgttgt
    44701 tcgccaggca gataaatagt caccgttcct tgcgcaaagg catggggtgt cattacgtaa
    44761 agtacgctgg caatgaaata cgactgtaat ttattcatgg tgcgggttta agaaacgtca
    44821 tttctacggg aataacccct gcgcctaaca tttgccgact ttgacgaacc tggccagtag
    44881 ccgaatcgac ccagaatgtg ttttgccact gtttttctgg ccgcgtggaa gtcacctctt
    44941 cctgccagac attacacagc acattgcgac ccgcaatatt cagcgtctct aatccagcaa
    45001 aggaaaaagt agaactcagg gtcgcagagc gaaaatggtt gtcttcactc cagagaatat
    45061 cgcgcgtcca gcggctgcca tcttttattg ccagcgcgtt acgcagaggg tcctgcccgg
    45121 aatttgtcac ttccagcaga ttattattaa gtttgacggt ttttaaaaga cgtccattgt
    45181 gggtaaccag catggcgtta tcctgggata accatttgct ttgttcttgt tctatatatc
    45241 ccagtaccac aaagattcgt tgcccttcat taaggcgtaa atacatcgtg gaataaggca
    45301 acgcctggat ctgctgatcc gctacggtga tatcctgatt atcgaaaagg ctggcgcgaa
    45361 atgtatcgac catactttgc tggctgtgcg tacatcccgc aagaaatagt gcgaaaatcg
    45421 ataaaataag agggcgcacc tttaatcctt gaaaggttaa ttaaaaaaat gcaaatcttt
    45481 aaagcccggt acgaatatcg caaccgggta tcatttaact ttaatttcga cgcgttaaag
    45541 cagtattagc gtgtagtgga cgtggtggtt gaggtgttat gagaaccgcc atcgccactg
    45601 tcatttgctg caacaactgc caacgtcgcc acaccaacag ccgttgccac accgacaact
    45661 gtcgccgtgc cgacagcgcc agcagccacg ccaccagtgg tagtagcagt cgtggttgcg
    45721 ccggtagtgg tcgttgtggt gccagtagta cccgtcgttg cctcggtccc gtttgttgct
    45781 tcaggggcag caaacgcagc tggcgtcgtc aacatgaagg ccataaggat tgcagaaagc
    45841 ttgtgtttca taacttttcc tttattcatc gcatggacaa tacgtacgct tgagatcagg
    45901 attgtctact ttgagtataa catccttgtg aaatctccct ttcctcatac cgcttgcact
    45961 atatactgcg ggtaggaaag gcgcgcagag ggaaatataa gattgttaac tatccccctc
    46021 tggaaatacg acttgtgagc ataagataaa tcttagcaaa atacaaatta aagataagga
    46081 aattgttacg aaagctatta attattgctg gtaatatagt ttcaagtggt actataactg
    46141 gctgttaaat ggcatttata actattaggt gcagagatat tcgcttaaat ggattagttt
    46201 ctttgtggaa aagtaactga tgttataata tatgatgttg actattgcac tattccaata
    46261 aaaccagtca gctttaaaca agcagcgtca tattaagaga gataaacatt tgccgctgtt
    46321 ggtcctcgca ggccatttac gcggcaaaat tccacacgta atcctggtat aagcacttct
    46381 gcgtcgcggg gagtgaatgc ggaaatatgg acctgaactt ctttacgacc gtcggagggg
    46441 ataatgaatc ctttgccgct tttgcgatca aaggttttga caattcctgt cattttacgg
    46501 gacaaacaaa ttccttactg aaaatactgc gctgcactat acggggttaa taaaataaag
    46561 ccagcgatat ttaagaccgc cggacggcta aaataaaatt tgcttaatct caattatcat
    46621 gcgttaatag ctgcgtcggt ttgaaagaca gacagcatac aaagtagttt actaaagcag
    46681 ttctcattat caggcattat ccccttcttt tgagtctctc tcctgaacac taagtagttt
    46741 ctgtattaaa gccctgtttg ccgaaaggcc caaaatgaag gaagtaaaat atgtctaata
    46801 aaatgactgg tttagtaaaa tggtttaacg cagataaagg ttttggcttt atcactcctg
    46861 atgatggcag caaagacgtt ttcgtccatt tcactgccat ccagagcaat gaattccgca
    46921 cgctgaacga aaatcagaaa gttgaatttt ctattgagca ggggcaacgt ggccccgcgg
    46981 cagcgaacgt tgttacgctc taaggttgcc gttattactc aacatctcca tttccgctgt
    47041 ccatgttgtc atggttcaca gtaccgcaca tcggcattcg atgtgacgga gcgaaatcct
    47101 ttgggcgcta agtgtatttt ttgtaaatcg acgatgatca cctttgataa cgtcacgctg
    47161 caaatacgca ctgaccatgc gccgctggat ttcacaaaat aatatcaggc tccctcg
//
LOCUS       DADLFA010000007        43141 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000007 DADLFA010000000
VERSION     DADLFA010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43141
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.007"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..255
                     /locus_tag="JQH65_000359"
     CDS             <1..255
                     /locus_tag="JQH65_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HAY6137878.1"
                     /translation="IRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQR
                     YFLALEEGVNLLPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(305..1561)
                     /locus_tag="JQH65_000360"
     CDS             complement(305..1561)
                     /locus_tag="JQH65_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HAY6137879.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(1763..2842)
                     /gene="mltC"
                     /locus_tag="JQH65_000361"
     CDS             complement(1763..2842)
                     /gene="mltC"
                     /locus_tag="JQH65_000361"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HAY6137880.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(2907..3182)
                     /locus_tag="JQH65_000362"
     CDS             complement(2907..3182)
                     /locus_tag="JQH65_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HAY6137881.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(3210..4262)
                     /gene="mutY"
                     /locus_tag="JQH65_000363"
     CDS             complement(3210..4262)
                     /gene="mutY"
                     /locus_tag="JQH65_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAY6137882.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMACFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            4423..5142
                     /gene="trmB"
                     /locus_tag="JQH65_000364"
     CDS             4423..5142
                     /gene="trmB"
                     /locus_tag="JQH65_000364"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAY6137883.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLNNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            5142..5468
                     /locus_tag="JQH65_000365"
     CDS             5142..5468
                     /locus_tag="JQH65_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF469 domain-containing protein"
                     /protein_id="HAY6137884.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            5652..6371
                     /locus_tag="JQH65_000366"
     CDS             5652..6371
                     /locus_tag="JQH65_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HAY6137885.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            6547..7593
                     /gene="ansB"
                     /locus_tag="JQH65_000367"
     CDS             6547..7593
                     /gene="ansB"
                     /locus_tag="JQH65_000367"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HAY6137886.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            7710..8717
                     /locus_tag="JQH65_000368"
     CDS             7710..8717
                     /locus_tag="JQH65_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HAY6137887.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQSAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(8781..9917)
                     /gene="hemW"
                     /locus_tag="JQH65_000369"
     CDS             complement(8781..9917)
                     /gene="hemW"
                     /locus_tag="JQH65_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HAY6137888.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEAERFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRAEFSAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(9910..10503)
                     /locus_tag="JQH65_000370"
     CDS             complement(9910..10503)
                     /locus_tag="JQH65_000370"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HAY6137889.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(10511..10801)
                     /locus_tag="JQH65_000371"
     CDS             complement(10511..10801)
                     /locus_tag="JQH65_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HAY6137890.1"
                     /translation="MNAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            complement(10798..11364)
                     /gene="yggT"
                     /locus_tag="JQH65_000372"
     CDS             complement(10798..11364)
                     /gene="yggT"
                     /locus_tag="JQH65_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HAY6137891.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNILLPGMWMAL"
     gene            complement(11382..12086)
                     /gene="yggS"
                     /locus_tag="JQH65_000373"
     CDS             complement(11382..12086)
                     /gene="yggS"
                     /locus_tag="JQH65_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HAY6137892.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            12104..13084
                     /locus_tag="JQH65_000374"
     CDS             12104..13084
                     /locus_tag="JQH65_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HAY6137893.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDALDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVGEGRL"
     gene            complement(13260..13676)
                     /gene="ruvX"
                     /locus_tag="JQH65_000375"
     CDS             complement(13260..13676)
                     /gene="ruvX"
                     /locus_tag="JQH65_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAY6137894.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(13676..14311)
                     /locus_tag="JQH65_000376"
     CDS             complement(13676..14311)
                     /locus_tag="JQH65_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708713.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HAY6137895.1"
                     /translation="MTAPFFPRILGAVAFLNQETEPLTMNLQHHFLIAMPALQDPIFR
                     RSVVYICEHNTNGAMGIIVNKPLENLKIEGILEKLKITPEPRDESIRLDKPVMLGGPL
                     AEDRGFILHTPPSNFASSIRISDNTVMTTSRDVLETLGTDKQPSDVLVALGYASWEKG
                     QLEQEILDNAWLTASADLNILFKTPIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(14348..15298)
                     /gene="gshB"
                     /locus_tag="JQH65_000377"
     CDS             complement(14348..15298)
                     /gene="gshB"
                     /locus_tag="JQH65_000377"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HAY6137896.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVLLMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            complement(15311..16042)
                     /gene="rsmE"
                     /locus_tag="JQH65_000378"
     CDS             complement(15311..16042)
                     /gene="rsmE"
                     /locus_tag="JQH65_000378"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAY6137897.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(16122..16829)
                     /gene="endA"
                     /locus_tag="JQH65_000379"
     CDS             complement(16122..16829)
                     /gene="endA"
                     /locus_tag="JQH65_000379"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HAY6137898.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(16924..17421)
                     /locus_tag="JQH65_000380"
     CDS             complement(16924..17421)
                     /locus_tag="JQH65_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HAY6137899.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MENVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(17498..18892)
                     /locus_tag="JQH65_000381"
     CDS             complement(17498..18892)
                     /locus_tag="JQH65_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAY6137900.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            complement(19328..20482)
                     /gene="metK"
                     /locus_tag="JQH65_000382"
     CDS             complement(19328..20482)
                     /gene="metK"
                     /locus_tag="JQH65_000382"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAY6137901.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATTFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            complement(20786..21001)
                     /locus_tag="JQH65_000383"
     CDS             complement(20786..21001)
                     /locus_tag="JQH65_000383"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6137902.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            21137..21268
                     /gene="yqgB"
                     /locus_tag="JQH65_000384"
     CDS             21137..21268
                     /gene="yqgB"
                     /locus_tag="JQH65_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HAY6137903.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            21277..23253
                     /gene="speA"
                     /locus_tag="JQH65_000385"
     CDS             21277..23253
                     /gene="speA"
                     /locus_tag="JQH65_000385"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HAY6137904.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            23390..24310
                     /gene="speB"
                     /locus_tag="JQH65_000386"
     CDS             23390..24310
                     /gene="speB"
                     /locus_tag="JQH65_000386"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HAY6137905.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(24516..25274)
                     /locus_tag="JQH65_000387"
     CDS             complement(24516..25274)
                     /locus_tag="JQH65_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAY6137906.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            25552..27543
                     /gene="tkt"
                     /locus_tag="JQH65_000388"
     CDS             25552..27543
                     /gene="tkt"
                     /locus_tag="JQH65_000388"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAY6137907.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            27857..28300
                     /gene="cmtB"
                     /locus_tag="JQH65_000389"
     CDS             27857..28300
                     /gene="cmtB"
                     /locus_tag="JQH65_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HAY6137908.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISHR"
     gene            28328..29716
                     /locus_tag="JQH65_000390"
     CDS             28328..29716
                     /locus_tag="JQH65_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HAY6137909.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSLGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSTNAVKAMKQEGAFSLSRVKRIAFVCDTGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            29731..31008
                     /locus_tag="JQH65_000391"
     CDS             29731..31008
                     /locus_tag="JQH65_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAY6137910.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLVEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPVQTLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGIDIGEAAQ"
     gene            31005..31970
                     /gene="yggF"
                     /locus_tag="JQH65_000392"
     CDS             31005..31970
                     /gene="yggF"
                     /locus_tag="JQH65_000392"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HAY6137911.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGVIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQESKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            31992..32501
                     /gene="fumE"
                     /locus_tag="JQH65_000393"
     CDS             31992..32501
                     /gene="fumE"
                     /locus_tag="JQH65_000393"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HAY6137912.1"
                     /translation="MAKLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQD
                     ETPGFADDMTWDFISNVNSITRNAMLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTILKELTP"
     gene            32498..33211
                     /locus_tag="JQH65_000394"
     CDS             32498..33211
                     /locus_tag="JQH65_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HAY6137913.1"
                     /translation="MKIELTVNGLKIQAQYQNEEIENVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(33183..33860)
                     /locus_tag="JQH65_000395"
     CDS             complement(33183..33860)
                     /locus_tag="JQH65_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HAY6137914.1"
                     /translation="MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(33854..34531)
                     /locus_tag="JQH65_000396"
     CDS             complement(33854..34531)
                     /locus_tag="JQH65_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAY6137915.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(34519..35226)
                     /locus_tag="JQH65_000397"
     CDS             complement(34519..35226)
                     /locus_tag="JQH65_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAY6137916.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLRILVIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            complement(35227..35805)
                     /locus_tag="JQH65_000398"
     CDS             complement(35227..35805)
                     /locus_tag="JQH65_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAY6137917.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(35828..36259)
                     /locus_tag="JQH65_000399"
     CDS             complement(35828..36259)
                     /locus_tag="JQH65_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY6137918.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYARQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            36631..37650
                     /gene="epd"
                     /locus_tag="JQH65_000400"
     CDS             36631..37650
                     /gene="epd"
                     /locus_tag="JQH65_000400"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HAY6137919.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            37700..38863
                     /gene="pgk"
                     /locus_tag="JQH65_000401"
     CDS             37700..38863
                     /gene="pgk"
                     /locus_tag="JQH65_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAY6137920.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            39078..40157
                     /gene="fbaA"
                     /locus_tag="JQH65_000402"
     CDS             39078..40157
                     /gene="fbaA"
                     /locus_tag="JQH65_000402"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HAY6137921.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLSFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            40515..41375
                     /locus_tag="JQH65_000403"
     CDS             40515..41375
                     /locus_tag="JQH65_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HAY6137922.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            41514..42149
                     /gene="argO"
                     /locus_tag="JQH65_000404"
     CDS             41514..42149
                     /gene="argO"
                     /locus_tag="JQH65_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HAY6137923.1"
                     /translation="MFSYYFQGLDLGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            42242..42982
                     /locus_tag="JQH65_000405"
     CDS             42242..42982
                     /locus_tag="JQH65_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HAY6137924.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
BASE COUNT        10551 a        10756 c        11500 g        10334 t
ORIGIN      
        1 attcgcggtg aagtggagtt ggtgcggatt cgtgatgccg aagggcgaat tgcggcagaa
       61 ggggcgttgc cttatccacc tggcgtgctt tgcgtggtac ccggggaagt ctggggtggg
      121 gcggttcaac gttatttcct tgcactggaa gaaggggtaa atttgttgcc gggattttcg
      181 ccggagctgc aaggtgttta tagcgaaacc gatgcggatg gcatgaaacg gttgtacggt
      241 tatgtgttga agtaagaata aaaaaaacgg gtcaccttct ggcgacccgt ttttctttgc
      301 gtaattagtg gctaaccgtc tgtgtgcctg tcgggacacg aacgtgttta tatttgaaca
      361 tcgccatgaa cgcgaaggcc agaaccacgg agtaaccagc gaaaatcaac catacggtct
      421 gccagtcggt aatgccgttt tgggtgtaca tctcaacaac tttaccgctc acgatgccgc
      481 cgaggataca gccgaagccg ttagtcatca tcaggaacat cccttgtgca ctggcgcgaa
      541 ttgccgggct aacttctttt tcgacaaaca ccgaaccaga gatgttgaag aagtcgaatg
      601 cgcaaccgta aacgatcatc gacagtacca gcagtacagt accgaacgga gtcgggtcgc
      661 cgtaagcaaa cagcgcaaaa cgcaggatcc acgccacaat actgatcatc attacgttct
      721 taataccgta gcggcttaag aagaacggga tggtcagaat gaacagggtt tcagagatct
      781 gcgaaatcga catgatgatt gacgcatgct gcacaataaa gctgctggca aacatcggat
      841 ctttgtcgaa gctgtgcagg aaggtattac cgaacatgtt ggtaatctgc agttccgcgc
      901 ccagcagcat tgagaagata aagaagattg ccatacgctt gtttttaaac agcgcgaatg
      961 catcgaggcc cagcagggtt gtccagctct gattcgcttg ctgtttagca accggaatat
     1021 gcggcagagt cagggtaaac agaaccagaa tggcggaaag tgctgcgcca atatacagct
     1081 gcatgtggct taattcgaag ccagacaggc tcaccaccca cattgccatg ataaagccga
     1141 tggtgcccca gatacggatt ggcgggaagt cagtaacgat atccatcccg gcattttgca
     1201 ggcgatagta agagatggtg ttgattaacc caagcgttgg catataagca aacgagttaa
     1261 tcaatatcac aaggaacatc gcctccggtg tcgtgacctg tgccgccatg aacagcgtga
     1321 tagcgccaat ggtgtggcaa atggcatata cccatttcgc acttaaccat ttgtcggcca
     1381 caatccccag cagcgcaggc ataaagaccg ctgcgatacc cagtgagcta taaactgcgc
     1441 caatagaagc accgtcaaac ttcagggtaa caaacatata ggagccgagg gtcgtcagcc
     1501 aacttcccca cagacagaac tgcagaaaag agaggatttt cagctgcagc ttaagattca
     1561 tgttaatttc ctcacatcgt gatgcggatg agtgtttgaa aaagcacatt tgaccgtgcg
     1621 agatgatggg attctatcaa cttcgcggac tggttttttg ttacctgtgg caaataattg
     1681 caaacattcc caatttgcaa aacgaaattg tgatgtggat aacatttttg cccctgagca
     1741 tcgtcagggg cggttaatgg aattatcggc ggcggtagga tttttgcgcg gtattcactt
     1801 tataaagata acggcgagat tccgcagagg gatggcgggt cgtcagcgtc tgataaacat
     1861 cgcccggcgt catggtgtta ataatattgg cagcctgaat cttatcattc gaaaagactc
     1921 gcagcacgct gcctgcgccg ccgttatagg cggtgatgac ggcataacgc cgcgatgttg
     1981 ggttatcaat tccgccgaga taaacattgt tcagcatcgc cagatacgcg gtgccggtat
     2041 caatattgct ggcaggatca aacaagaaac tgcggctcgg cgtgccggat ttcccctgcg
     2101 agcggaacac atctttcccg gcagtatgtt gtaccacctg cattaatccc agcgcatcgg
     2161 aacggctgac cgcatacggg ttaaaggaag attcggtctg cataattgcc agaatcagcg
     2221 actcatcaac gccatatttc cgtgacgcct ggcggaccat gccgagatat ttgtgcgcac
     2281 gtttatcaag gtggttcggt accatgttaa tggtgacgct gtagatgata cgcagcccgt
     2341 tactgcggct ttgcagacgg tttttcagca gataatccgc gaagttgctt gcgcgacctt
     2401 cccagcgaat cggctgcccg gtgttgtcca ccacctgacc gtaaaggaaa ggttctttcg
     2461 aaatcgtaat atcatcaacg tcggaataga gatcgaccga actcggatcg tcacccatca
     2521 gcaacgtttt gataattgcc cggcgcaaat gcgcggcagg ttctgtcccg gcgatagttt
     2581 cgatagtaat cgtaccgtca tcgaagttga tgtggctgcg ggtctgatat tgatcggtgt
     2641 atttcacgta gtccttagga ccggcgatca ccacctcttt gaatccccag atgttctcaa
     2701 tattgtgggc aaattgcccc atcagaatat caaaaccgtt ggtatctttg acccaggctt
     2761 cgttataggt atcgcctttt ttggtcgtcg aacaggagat gagcaacggc gcaatcaaag
     2821 ccagcgcgag atattttttc atcattccgg gtgcgtgttg tgtttgtttt tatgcaagtt
     2881 gccggaggcg tgctccggca ctgtttttat tttttatctt ccggcgtata gccctcgata
     2941 tgcacctctt taccctcgaa caggaagttg accatctcct gctcaagcag cttgcggtgc
     3001 tcggcattca tcatgttgag tttcttttca ttaatcagca tggtttgctt gtgctgccac
     3061 tgcgcccagg cttctttgga gatctcgtta tagatgcgtt ttcccagctc gccggggtac
     3121 agctgaaaat cctgaccttc tgcttcacgt tgcaggaaag tacaaaaaat cgttctgctc
     3181 ataaatcatc ctctttatcg actcacgcgc taaaccggcg cgccagtgcg taactgctgt
     3241 aacaaacgct ccacgggagc cgccaggcca actgacggcg gttgcgctaa gttataccag
     3301 agcgcattgc cttcatccat gcagccggtg aatgacgaca cgggaagcca cataggcaca
     3361 atatctaagt ggaaatggct gaaggtatgc cgaaacgcgg tcagttgcgt caggttatcg
     3421 gcagcaatct gccgttgcgc cagccactgc cgcaaacttt cttcgtcggc aaactgcggg
     3481 aaacagtata aaccgcccca caatccgctc ggcggacgct gcgccagcaa tacttcatct
     3541 tcgtgctgta acagcaaaaa gtagccggtg cgctccggca gcgtctgttt cggttttttg
     3601 cccggataaa gcgaccagct attattggcg gcggcaatac atccgttttg tagcggacag
     3661 agcgaacatt tcggcttcga gcgcgtacaa atcatcgcgc ccaaatccat catcgcctga
     3721 ttaaaccgtt ccacgccgac cgcgggcgtc acctgctcgc tcaaactcca taatttattc
     3781 tcgacctctt ttttcccagg ccagccgctt acagcatagc agcgcgccag cacgcgtttg
     3841 acgttaccgt cgagaatcgg aaagtgctta cccagagaaa gcgagagaat cgcgcctgcg
     3901 gtggaacgcc cgacgcccgg taacgccgcg acttcttcaa aggtttccgg gaatttaccg
     3961 ccgtgtaagg tcgccacttg ttgtgccgct ttatgcagat tgcgcgcgcg ggcgtaatag
     4021 ccaagcccgg tccacaagtg gagaacttcg tcgagcggcg cattggcgag atcggtcacc
     4081 gtcgggaagc acgccataaa gcgttcaaaa taggggataa cggtcgcaac ctgagtttgt
     4141 tgcaacatca cttctgagag ccatactttg tagggcgtct tgtcaatttg ccagggcagc
     4201 gtttttcgcc cgtatttatc gtaccagtcc agaacctggg ctgaaaattg cgacgcttgc
     4261 atggtcaccg aattcactgt tgttgggggc aagattgcag cacagcgacg gcagggtgta
     4321 aaccggaact ttccgcagcc acggccttat catgcttgca tccggcaact aactttggat
     4381 aatgcccgtt ttcagaacac tttcacaagc gactaaatct ttatgaaaaa cgacgtcatt
     4441 tcaccggaat ttgatgaaaa cggccgccca ctgcgccgta tccgtagttt tgtgcgccgc
     4501 caggggcgac tgaccaaagg ccaggaacat gcgctggaaa actactggcc ggtgatgggc
     4561 gttgagttca gcgaagatat gctggatttc cccgcgcttt ttggccgtga agcgccggtg
     4621 acgcttgaga ttggttttgg catgggggcg tcgctggtgg caatggctaa agatcgccct
     4681 gagcaggact tcctcggcat tgaagtgcat tcaccgggcg ttggtgcgtg cctggcttct
     4741 gcgcatgaag aaggtttaaa caacctgcga gtgatgtgtc acgatgcggt tgaagtgctg
     4801 cataaaatga ttcctgacaa ttcattgcgc atggtgcagc tctttttccc tgacccgtgg
     4861 cacaaagcgc gccataataa acgccgtatc gtacaggtgc cgtttgccga actggtaaaa
     4921 agcaaactgc agctgggggg cgtattccat atggcgaccg actgggaacc ttatgcggaa
     4981 catatgcttg aagtgatgtc ttctattgac ggttataaaa acctgtcaga gagcaatgat
     5041 tacgtaccgc gtccggcatc acgtccggtg acgaaatttg aacaacgtgg tcatcgtctt
     5101 ggtcacggag tatgggactt aatgttcgag agggtgaaat aatggcaaag aaccgtagcc
     5161 gtcgtctgcg taaaaaaatg cacatcgacg aattccagga attaggattt tcggtggcat
     5221 ggcgattccc ggaaggtaca tcggaagaac agattgataa aaccgttgat gattttatta
     5281 acgaggttat cgaaccgaac aaactggcct ttgacggcag cggttatctg gcctgggaag
     5341 gtctgatctg catgcaggaa atcggcaaat gcaccgaaga acatcaggcg attgtgcgta
     5401 agtggctgga agagcgcaaa ctggatgagg tacgcaccag cgaacttttc gacgtttggt
     5461 gggactaaga aagcatacgg gcgatgacaa atgcaaaact gcctgatgcg ctacgcttat
     5521 caggcctgga aagatgcacg atcgagtagg cgggataagg tgtttacgcc gcatccggca
     5581 tggaaaacgc gtactttgtt atcaatctgg ggccagcaaa tgctggcctg attttttctt
     5641 gagggaagac tatgatgcgc aaaatgctgc tggcggcagc actttcagtg acggcaatga
     5701 ccgctcacgc cgactaccag tgcagcgtca cgccgcgtga cgatgtgatt gtcagcccgc
     5761 aaaccgtgca ggtgaagggc gaaaacggca atctggtgat cacgccagac ggcaacgtga
     5821 tgtataacgg taagcaatat tccctgaatg ccgcccagcg cgagcaggcg aaggattatc
     5881 aggctgaact acgcagcacg ctgccgtgga ttgatgaagg cgcgaaaagc cgcgtcgaaa
     5941 aagcccgtat tgcgctggat aaaattatcg ttcaggagat gggcgaaagc agcaaaatgc
     6001 gcagccgtct gaccaaactt gatgcgcaac tgaaagagca gatgaaccgc attatcgaaa
     6061 cgcgcagcga tggcctgacg tttcactata aagccattga tcaggttcgc gccgaaggcc
     6121 agcaattagt gaatcaggca atgggtggaa ttttacagga cagcattaat gaaatgggcg
     6181 cgaaagcggt gctgaaaagc ggcggtaacc cattacagaa tgtgctggga agcctggggg
     6241 ggctgcaatc ctcaatccaa accgagtgga aaaagcagga aaaagatttc cagcagtttg
     6301 gcaaagatgt ttgtagccgc gttgtgactc tggaagatag ccgcaaagcc ctggtcggga
     6361 atttaaaata atcctctatt ttaagacggc ataatacttt tttatgccgt ttaattcttc
     6421 gttttgttac ctgcctctaa ctttgtagag ctccaaaata tattcacgtt gtaaattgtt
     6481 taacgtcaaa tttcccatac agagctaagg gataatgcgt agcgttcacg taactggagg
     6541 aatgaaatgg agtttttcaa aaagacggca cttgccgcac tggttatggg ttttagtggt
     6601 gcagcattgg cattacccaa tatcaccatt ttagcaaccg gcgggaccat tgccggtggt
     6661 ggtgactccg caaccaaatc taactacaca gcgggtaaag ttggcgtaga aaatctggtt
     6721 aatgcggtgc cgcaactaaa agacattgcg aacgttaaag gcgagcaggt agtgaatatc
     6781 ggctcccagg acatgaacga taatgtctgg ctgacactgg cgaaaaaaat taacaccgac
     6841 tgcgataaaa ccgacggctt cgtcattacc cacggtaccg acacgatgga agaaaccgct
     6901 tacttcctcg acctgacggt gaaatgcgac aaaccggtgg tgatggtcgg cgcaatgcgc
     6961 ccgtccacgt ccatgagcgc agacggtcca ttcaacctgt ataacgcggt agtgaccgca
     7021 gctgataaag catccgctaa tcgtggcgtg ctggtggtga tgaacgacac cgtactggac
     7081 ggtcgcgatg taaccaaaac caacaccacc gacgtagcga ccttcaagtc tgttaactac
     7141 ggtcctctgg gatacattca caacggtaag attgactacc aacgtacccc ggcacgtaag
     7201 cacaccagcg atacgccatt cgatgtctct aagctgaatg agctgccgaa agtcggcatc
     7261 gtttataact acgctaacgc atccgatctt ccggctaaag cactggtaga tgcgggctat
     7321 gatggcatcg ttagcgctgg tgtgggtaat ggtaacctgt ataaatccgt gttcgacacc
     7381 ctggcaaccg ccgcgaaaaa cggcactgca gtagtgcgtt cttcccgcgt accgacgggt
     7441 gctaccactc aggatgctga agtggatgat gcgaaatacg gcttcgtcgc ctctggcacg
     7501 ctgaacccgc aaaaagcgcg cgtcctgctg cagctggctc tgacgcaaac caaagatccg
     7561 cagcagatcc agcagatctt caatcagtac taatcacctc gctccggtat cgtgccgggg
     7621 ctttttcact tcagacttac gtccattgcc aattttcatt accctaatga taatcaccgg
     7681 aataaattat ttcgcgtgag gtttttcggg tgaaaaagca atggattgtt ggcacggcac
     7741 tgcttatgtt gatgactggt aatgcctggg cagatggcga accgccaact gaaaatatct
     7801 taaaagatca attcaaaaag cagtatcacg gcattctcaa gcttgatgct atcaccttaa
     7861 aaaatcttga tgctaagggt aatcaggcca cctggtcagc ggaaggcgat gtctcttcca
     7921 gtgacgatct ctatacctgg gtcggtcagt tggcagatta cgaactgctc gaacagacct
     7981 ggacgaaaga taaaccggta aaattctcgg cgatgttaac cagtaaagga acgcccgcgt
     8041 ctggctggtc ggtgaacttt tactcttttc agtcggcagc cagcgatcgt gggcgggtgg
     8101 tcgacgatat caaaacgaat aataaatatc tgatcgtgaa tagcgaagat ttcaattatc
     8161 gctttagtca gcttgaatct gcgttgaata cccagaagaa ctcgattcct gcgctggaaa
     8221 aggaagtgaa agcgcttgat aagcaaatgg tggcggcgca gaaagcagcg gatgcatatt
     8281 ggggaaaaga tgcaaacggt aagcaaatga cccgcgaaga agcctttaaa aaaattcacc
     8341 aacagcgtga tgagtttaat aaacagaacg atagcgaggc gttcgcggtt aaatatgaca
     8401 aagaggttta tcaaccggcg attgcggcat gtcataaaca gagtgaagag tgttatgaag
     8461 tgccgattca gcagaaacga gatttcgata tcaacgaaca acggcgacag acttttctgc
     8521 aatcacaaaa actcagccgt aaattgcagg atgactgggt aacgctcgaa aaagggcaat
     8581 atccgttaac gatgaaagtt tcagaaataa acagtaaaaa agtcgcgatt ttgatgaaaa
     8641 ttgacgatat caaccaagct aatgaacgct ggaaaaaaga taccgaacaa ctgcggcgca
     8701 atggggtgat taagtaagtt tgatatagtg cacgatttgt agcaggatgc ggcgttgacc
     8761 ccgcatccgg caatacaagt ttactcagcc agaaaaagct ccagcagcga atttaaaaac
     8821 agcttcccat gttccgttat ctgccagtaa tccgcacatt ctgtgagata accctgggca
     8881 atcgcctcgt ctaactgtgg gcgaatcaca tcttcgcaaa gcccggtata cgcactaaac
     8941 tctgcgcgtg gcgcggcctc cagcaagcga aagcggttca taaagaactc aaacggctta
     9001 tctgcggctt cgacatcacg ctggctttcc agataccttc cttgcataaa accacgagga
     9061 tgacgcgttt tagtggtacg cagaatgcgc ccatccggga aggtgacttt gccgtgcgcg
     9121 ccgcagccaa taccgatgta gtcaccaaat cgccagtagt tgagattgtg ctggcactga
     9181 taaccgggtt tggcgtaagc ggaagtttca tattgctgat aacccgctgc ggttaataac
     9241 tgatgcccct gttcgaaaat atcccacagc gcgtcatcgt ccggcagcac cggtggtcgc
     9301 gaaccaaaca gagtattggg ttcgatggtc agttgatacc aggaaagatg cggcggattc
     9361 agttcaatgg cctggcgtag atcgccaagc gcctcttcca gtgattgatc cggtagtcca
     9421 tgcatcaaat caaggttaaa gctacgtaac cctaaaccgc tcgccagctt tgccgctcgt
     9481 ttcgcttctt gcgggccatg aatgcgccca agtcgtttca gcttttcttc gctaaaactc
     9541 tgcacaccaa tagagatgcg gttcacacca gcacgctgat aatcgacaaa gcgctcggct
     9601 tctaccgtac cagggttcgc ttccatagta atttctgcat ccgctgccag cggcaaacgc
     9661 gcacgcacgc cgtccagcag cgtttgcatc gccgggccgg aaagcaggct cggcgtacca
     9721 ccgccaataa agattgtctt tacttcacgg ccctgagcgt aagccacatc attgtccaga
     9781 tcgcaaagca gatgctgaac ataatcgtcg tgcggcactt ctcctttcaa cgcgtgagag
     9841 ttgaaatcgc agtacgggca tttctgcacg caccacggga tgtgaatgta gagactcagc
     9901 ggcggtaatt taaccattac gtaaagcgtc cagcagcagt ttcaacgcct gaccacggtg
     9961 ggaaatggcg ctcttttctt cgcgggtcag ttcggcggcg gttttccctt cggaaggtac
    10021 gaagaagatt ggatcataac caaagccacc agtgcccgct ggttcgcgag taatcacgcc
    10081 cggccagctg ccgtggcata ccagcggagt cggatcttcc gcgtgacgca gatataccag
    10141 cacgcagtgg aaacgtgcct gacgttggtc gtccggtacg tctttcagtg tttccaacag
    10201 tttttgcaga ttcttttgat cggtcgcgtc ttcaccggaa taacgcgcgg agtaaatacc
    10261 cggcgcgcca cccagcgcat ctaccgccag accagagtcg tcggcaattg ccggtaaacc
    10321 ggtcactttt gccgcatggc gcgctttcag aatcgcgttt tcgataaagg ttaggccggt
    10381 ttcttcagcg gaatcaacgc cgaggtctgt ttgtgccacg atatcaagac caaagtcgct
    10441 aagcagcgac gccagctcac gcactttacc ggcattgccg gttgcgagga caactttttg
    10501 cataggatac ctaattaatt aacgccgcga tttctggcgg gatttgttgc ggattaatga
    10561 ttttaatttg tttgtggcgg ccaagttcgc ctttttcaat caccacctgg cttttggcaa
    10621 cccggaattg cttaccgaga aacttcacca gatgactgtt ggcctggccg tcaactggcg
    10681 gcgcggtaat ggcgacttta acttcgtcgc catgtaaacc gacaatagaa tcacggctgg
    10741 ctttcggctg aatatagagc cgtaaaacca gaccgtcatc attaactgtt acggcattca
    10801 taacgccatc cacatccccg gcagcaggat gtttccggtt gcctgtaata cttctgcgac
    10861 acccatattg atgacataca gcagcagaac gaggatcatc ggcgagaaat caatcccacc
    10921 cattgccggt agcaagcggc gaatcgggcg cagcagcgga tcggccagct gaatcagcac
    10981 gtattcaatc gggctacgac cctggcttac ccagctcata atcgccatca ccagcaggac
    11041 ccagaaaatc agcaagccga tggttttcag cagaatcagt aaaccggcaa tccagatgat
    11101 tggcaggaag gtcaccactt taaacagcac aatggctttg ataaaactga gaatataggc
    11161 aaccagcagc gaggcgctgt caatgggacc cattgccgga ataacgcggc gcagtggccc
    11221 gataattggc tgcgttacct tcactacaaa ctgtgagaag gggttgtaaa aatcacaatg
    11281 agcccactgc atccagatgc gtaataacag caccatggta tacagctcaa tgaccgttga
    11341 aagcaggaaa gtcaacgtat tcatggcgtt cctttaattc cttatttttt agagtaatca
    11401 cgcgcaccaa aaattgcagt gccgatacga accatcgtgc tacctgccgc aatggcggct
    11461 tccatatcgt ccgacattcc cagagagagc gtgtcgatat gcgggtagcg cgttttcagt
    11521 ccggcaaatg ctacagccat ttggcgtgca acttcaaact gccttacata ttctgactca
    11581 ggcgcaggga ttgccatcag cccgcgcaga cgtaaacgcg gtagttcagc gaccgccgct
    11641 gccagctcgt ccagttctgc cagttgaatc ccggacttac tgttttcatc actaatatta
    11701 atttgaatca gtacgttaag agggggaagt tctgccgggc gctgatcgtt gaggcgggta
    11761 gcaatgcgca aacggtcgat ggtatgacac cagtcgaaat gctctgccac caggcggctt
    11821 ttattagact gcaacgggcc aataaaatgc cattctaatc ctgttacgcc cagttcctga
    11881 aagtggcgaa ttttatctac cccttcctga acgtagtttt caccaaattg acgctgcccg
    11941 gcatcaatgg cttctgcgat ggcgctcgca ggttttgttt tactgactgc aagcagcgta
    12001 atttcttctg gagaacggcc gcaacgcgtt gcagccgctg agattttgtc ccggacctgt
    12061 gccaggttat gcgcaatatc gttcattttc cgaggatctt agtatgaata tggaagaaat
    12121 tgtggccctt agtgtaaagc ataacgtctc ggatctacac ctgtgcagcg cctggcccgc
    12181 acgatggcgc attcgcgggc taatggaagc tgcgccgttt gatgcgctgg acgtcgaaga
    12241 gctactgcgg gagtggctgg atgacgatca gcgggcaata ttgctggaaa atggccagct
    12301 ggattttgcc gtgtcgctgg cggaaaacca gcgattgcgt ggcagcgcat tcgcgcaacg
    12361 gcatggcatt tcgctggcgt tacggctgtt accttcgcac tgcccgcagc tcgaacagct
    12421 tggcgcacca acggtattgc cggaattact caagagcgag aatggcctga ttctggtgac
    12481 gggggcgacg gggagcggca aatctaccac gctggcggcg atggttggct atctcaatca
    12541 acatgccgat gcgcatattc tgacgctgga agatcctgtt gaatatatct atgccagcca
    12601 gcgatgtttg atccagcagc gggaaattgg tttgcactgt atgacgttcg catcgggatt
    12661 gcgggccgca ttgcgggaag atcctgatgt gattttgctc ggagagctgc gtgacagcga
    12721 gacaatccgt ctggcactga cggcggcaga aaccgggcat ttggtgctag caacattaca
    12781 tacgcgtggt gccgcgcagg cagttgagcg actggtggat tcatttccgg cgcaggaaaa
    12841 agatcccgta cgtaatcaac tggcaggtag tttacgggcc gtgttgtcac aaaagctgga
    12901 agtggataaa caggaaggac gcgtggcgct gtttgaatta ttgattaaca ctcccgcggt
    12961 ggggaatttg attcgcgaag ggaaaaccca ccagttaccg catgttattc aaaccgggca
    13021 gcaggtgggg atgataacgt ttcagcagag ttatcagcag cgggtggggg aagggcgttt
    13081 gtgacgttgc catgcccgat gcgcaagttt atagggccta taaaggcaat aaatgtctga
    13141 tgcgacgcta acgcgtctta tcagacctac agccgctgcc agatgtgtgc tggataaggc
    13201 gttcacgccg catccggcaa gatggcggta acgactatcc gccaggcttt tagatcgcct
    13261 taatatccct gctcgaaata gctttcgaga ataataaccg cagaggcaga gtcgactttg
    13321 cctttgttga gcgcccgata gccgccctgt tcaaacagac cggaacgggc ttccacggtg
    13381 ctaagacgtt cgtcatggag ctttacttca acaccgaaac gaccatgaat acggttggca
    13441 aatttacgcg ctctggcagt caatggttgc tcggtgccgt ccatattcag cggcaaaccg
    13501 acgatgattt cgtccggctg ccactctttc agtaaacgtt cgatgaggtt ccagtccggc
    13561 gtaccgtcct gtgctttaat tgcaggcaaa gggcgagcag tgccggtaat gcgttggccg
    13621 accgctacgc caatgctttt ggtgccgaag tcgaaggcga gtaaggttcc actcatcagg
    13681 cgtgccctgc cacaccaggc atggtgagaa tatccacacc aatcagtttt gccgcctcgc
    13741 gccagcggtc ggcaatcggc gttttgaaca gaatattcag atccgccgag gccgttagcc
    13801 acgcgttatc gagaatttct tgttccagtt gacctttttc ccaggaggca tagcccagag
    13861 ccaccaatac gtcagacggt tgtttatcgg tgccgagcgt ttccagcaca tcgcgggaag
    13921 tggtcatcac cgtgttgtct gaaatgcgaa tgctggaagc aaaattggag ggcggagtat
    13981 gcaaaataaa cccgcgatct tcagccagtg ggccgccgag cataaccggt ttatccagac
    14041 ggattgattc atcacgcggc tccggcgtga tcttcagctt ttccagaatc ccttcaattt
    14101 tgagattttc cagcggcttg ttgacgatga tccccattgc accattggta ttatgttcgc
    14161 aaatgtagac cacggaacga cggaaaatcg gatcctggag agcaggcatg gcaataagaa
    14221 agtgatgctg taaattcatt gtcagaggtt ctgtttcctg gttcaaaaaa gcaacagcgc
    14281 ccagtatgcg tggaaagaag ggcgctgtca ctcagagtct caacgagatc cttctcgcta
    14341 aggtgggtta ctgctgctgt aaacgtgctt cgatggcatc cattaacatt ccggtgatcg
    14401 acaccggaaa ctctgcttca atctcacgaa tacaggttgg gctggtgacg ttaatttcag
    14461 tcagacggtc gccgatgata tccagaccaa caaaaatcag ccctttttct ttcagcgtcg
    14521 gcccgatctg acgggcgatt ttccagtcac tttccgtcag cggccgaggt tcaccgcgac
    14581 caccggcagc cagattgcca cgggtttcgc ccccctgcgg aatacgcgcc aggcagtacg
    14641 gtaccggctc gccatccacc accagcacgc gtttgtcgcc atctttaatg gctggcaggt
    14701 aattttgcgc catgcagtag cgagtgccat gctcagtcag ggtttcggca atcacgccga
    14761 ggtttggatc gccttctttc acgcggaaaa tcgacgcgcc gcccataccg tccagcggct
    14821 taagaatgat gtcgctgtgt ttctcccaga acgcttttag ctgcgcttta ttgcgcgtaa
    14881 ccagcgtttc tggcgttaag tcagagaacc aggcggtaaa cagtttctcg ttacagtcgc
    14941 gcaggctctg cggcttgtta acgatcagcg tccctttctc ttcggcacgt tccagaatat
    15001 aggtcgcgta gataaactcg gtatcaaacg gcgggtcttt acgcatcagg agcacatcga
    15061 gatcggccag cggcagatcc tgttcaccga cgaacgaaaa ccactcttcg tagttctgct
    15121 tcacgttcag cgtgcgggta tgggcgcgag cttcgccatt gatcagatac agatcgccca
    15181 tctccatata gtgaagttcg taaccacgac gctgtgcttc cagcaacata gcgaaactgg
    15241 aatctttctt gatgttgatg tttgcgatgg ggtccatcac gatgccgagc ttgatcatta
    15301 ttcttctccg ttagcccaaa tcgccaaatc gtacttgtag cgcggtaatg gcggtgagcg
    15361 cagttgtctc tgtacgcaaa acgcgaggtc ccaacaggat atcagtaaat tgatagcggg
    15421 cagtcatggc aatttcatct gccgataaac cgccttccgg gccaatcagc aggcggacgc
    15481 gttcaaccgg taacggcaac gtattgatgc tgttactggc gcgcgggtga agattcagtt
    15541 tcagtccttc atcctgctct gcacaccagg cttccagatc catcgctgga cggatttccg
    15601 gcacccggtt acgaccacac tgctcacagg cagcaattgc aatcttctgc cactgctgga
    15661 gcttcttgtt cagacgttca ctatccagtt taacgccgca gcgctcagaa aaaagtggcg
    15721 taatgaggct tacaccgagt tcgatcgatt tctggatcgt aaattccatt ttttcaccac
    15781 gcgacatcac ctgaccgagg tgaatatgca gcggagattc gcgatcgtcg atcttgcctt
    15841 ccagcacctt cacttccacg ctttttttgc tggcgctggt aatttcggcg tcaaagacct
    15901 ggttgctacc gtcaaacaat tgcaacgcct gccccggccc catgcgcagt acgcgcccga
    15961 tatggttggc ggcatcttcg caaagcgcga tgtgagaatg gctggtcagt ggttcaggat
    16021 gataaatgcg ggggatacgc atagttaaaa atccgcgtcg tctccccacg cggttgtacg
    16081 cgtggggtag gggttaacaa aaagagtccc gctagtgtag gttagctctt tcgcgcctgg
    16141 caagcgcgtt gcacatacgg gttatgattg ccttgcacct tcgcgatgcg ttcatcgcgc
    16201 tcgcactccc agtcggtaac cggatacatc ttgttccatg cgttgaacag ctgcgtttgc
    16261 tggcgagaga gtgtcaggtt gtattggtcg cgcatataga agtaggtgcg cgcaatggca
    16321 ccgcgtgcac gcgctggtgg ttcggcagct ttttctttga aatcgacctt catggcgcat
    16381 tgaccgtact ggccttcgcc gccattccac tggctgtaca taaagttgcc gcgatcgcca
    16441 ttcacctcac cgactgacgg ctgcaggtta tgcatatcgc tttccatctt gcgatagacc
    16501 ggatctttag cgcagttttt acgtccaccg tcctgccagc actggcgctg gtgaccgaac
    16561 tgccaggcgg gaacgacgtg ttcccactct acgcggctgg ctcggttttc atttttgcgc
    16621 acctgatagc cgcacgattg cagatcaaca acgccttttt tgccctgcca gttaatttta
    16681 catccgcaat aaaacgtacc gggcgcgtca gcgtggactt ttaccgccgc ggctttcgcc
    16741 tgagaaaaac tattgatacc ttcggccaac gccgggccgg aaaatgctgc gctcagtacc
    16801 accgcagcaa tagacaaata acggtacatc acttactccg tgttaaaacg agccagcaac
    16861 gtagcgaaag ttgttttggt atgcaatcag ccagatcagg aaagttcctg atagttcaga
    16921 tggttatttt gcgaccagtt gttcgccgca gtgaacacag cgatagacgg cttcgccacg
    16981 aactacgcga ttatggcggc gtacggtaag ctgatgctcc tggcacttgc agcggtaggg
    17041 gaaggtgttg cgacgcacgg attgcagttc gaactgatgc gtacgacggg cgggaacacc
    17101 cagcacgttt tccatcatcc acttccactc tttgccatgt ggtgctacgc gcccgaaatg
    17161 tttccatacc agcaaatgtg ccagttcgtg cggcaccact tcttcaataa aggcttcact
    17221 gttttccaac agcaaaacgg gattgaggcg gatttcatag ctttccagcc aggctgttcc
    17281 ggcggaggtg ccgcgctggg tataagagag ttttggctcc gggtagttac gccccaattt
    17341 caggttagcc tgggcgagtt tttcccgcag gcgacgcata acggcctgtt ggatggcgat
    17401 agggagacgg gatgttttca tagcggcaga ggatagagcg aagaaaagat gactgcaaga
    17461 ggtggcttcc tccgcgatgg gaggaagctt ggggagatta atcgtgagcg cctatttcgc
    17521 gcagtttacg acctttcatc agattacgtt caatatgttc cagcgaaacg tgtttggttt
    17581 ccggtaccag ccacaatgtc agcaggataa acagtacgtt cagagccgca tacacccaga
    17641 aggtgttggc gttacccagt gtgttgagca tggtcaggaa cgttgcgcca acgatcatgt
    17701 tggcaatcca gttggtggcg gtagagcagg tgatgccaaa atcgcggcct ttcagcggct
    17761 gaatttcgga gcacagtacc caaatcagcg gaccggcact catggcaaaa ccgacaataa
    17821 acatcagcag catggcgatg gcgaaatact gcgctgacgg agagtgaata ccgatatgca
    17881 tcattgtacc gagtacgccc atgccagcag ccatcaccag gaagcccagc gttagcgttg
    17941 gtttacgtcc ccagcggtca acaaggccga ttgcgataaa ggtggcaagt acgttggtca
    18001 ggccgacaat cactgtcccc cacatttgct cggtagtgtt ggtataaccc gccagttcga
    18061 agattttcgg cgcgtaatac atgatgacgt tcatcccggt gaattgctgc attacctgta
    18121 acagtacgcc aaggaacacc gcgcggcgga agttgctatt ctctttaaac agcgcccagc
    18181 cactctgttt aacctgcaaa ctttcacgga tttcatccag ttcgcgtttc gcttccgcgc
    18241 tggtgtcacg caggcgtagc agcacgcgtt cggcatcaac aaaacggcgt ttggcggcaa
    18301 accaacgtgg gctgtctggc aggaagaaga caccaatcag cagcaaaatt gccgggatga
    18361 taatcacacc cagcatccag cgccatgcac cggtgtagct gaaggcggta tcagaaagat
    18421 aagcaccgag gatcccgata gtgatcatca actgatacat cgagatcata ctgccacgaa
    18481 ttttttccgg cgcaatttca gagaggtaca gcggtgcggt ataagaggcc acacccaccg
    18541 ccagccccag tagaacgcgg gaaagaatca gtacttcaac gtttggcgca gccgcagaga
    18601 acagcgaacc ggcaacaaac aaaattgcgc cgatcatcag gctctttttg cgcccgagtt
    18661 taaaggagag ccagccgctg cccaccgcac cgactgccgc accgaacatc atggagctta
    18721 cgacccattc ttgcgtgtgc gaagtaatct ggaattcatc tgcaataaac ggcagtgcgc
    18781 cagcaattac accgatatcc aggccaaaga gtaatcccgc cagagcggca aggaagcaga
    18841 cgaaaaacgt cattgccttg tttgaccgcc cctgtttttt agcgtcaggc atgatgccct
    18901 ccaatatggt tattttttat tgtgaattaa gataggtgag tacgacgtaa aaagatgtga
    18961 agcaaatcac atcagtgtaa tcgcttacac taacatttat cttagttatg gtttaagtgc
    19021 atgaaaataa ttgagttata tcatcataga ttaaacgctg ttatctgcaa ttaagacttt
    19081 actgaaaaga aatgtaacaa ctgtgaaaac tgaataataa tatgaaaata ttacaaatat
    19141 ttggctggtg ttattggtgt aatcggtttc agtagtgatg ataatttgcg ataaaacgtg
    19201 ttgaggaaga atgatgttat tgtgcgggca gctttttgcc ggatgcggcg tgaatgccta
    19261 tccaccgcgg tgaggtaaaa gaaaaaggcc agcgcgaggc tggcctttga aagcaggtga
    19321 agaaagatta cttcagaccg gcagcatcgc gcagcagctg cgctttgtcg gttttttccc
    19381 acgggaaatg ttcacgacca aagtgaccgt atgctgcggt ttctttgtag atcgggtgca
    19441 gcagatccag catctgaatc agaccgtatg ggcgcaggtc gaagaactca cgtaccagca
    19501 gagtcagttg ttcagaaggc actttctcag taccgaaagt ttctaccatg atggaggtcg
    19561 gttcagccac gccgattgcg taggaaacct gaatttcaca acgatcggcc aggccagcag
    19621 caacgatgtt tttcgcgaca taacgtgctg cgtaggctgc ggaacggtcc acttttgatg
    19681 gatctttacc agagaatgct ccgccaccgt gacgtgccat gccgccgtag gtatcaacga
    19741 taattttacg accagtcaga ccgcagtcac ccatcgggcc accgataaca aaacgaccgg
    19801 tcgggttgat gaagaatgtg gtggcagaag tcagccattc agcgggcaga attggcttga
    19861 tgatctcttc cattaccgct tcttgcagcg atttctggtc gatctcttca gagtgctgag
    19921 tggaaagcac gacagcatca ataccaacga ttttgccgtc gtcatactgg aaagtcacct
    19981 ggcttttcgc gtccgggcgc agccacggca gagtgccgtt tttacgcact tcagcctgac
    20041 gctgtaccag acggtgtgca taggtgatag gtgctggcat cagcacgtcg gtttcattag
    20101 ttgcgtagcc aaacatcaga ccctggtcac ccgcgccctg ttccagcgga tcggcacggt
    20161 caacgccctg gttgatgtca ggagactgtt tgccgatagc gctcagaacc gcacaggagt
    20221 tagcgtcaaa gcccatgtcg gaatgcacat agccaatttc gcgaacggtg ttacgagtga
    20281 tctcttcgat gtctacccag gcgctggtgg tgatttcgcc gccaactaaa accatgccgg
    20341 tttttacgta ggtttcgcaa gcaacgcgtg ctttcggatc ctgttcgagg atcgcgtcta
    20401 aaacggcatc agagatttgg tcagcaattt tgtcaggatg cccttcagag acggactcgg
    20461 acgtaaaaag gtgttttgcc atatttaata tcacctaaag agaatttggt tagctcaaac
    20521 tgttgtgtgg attttctgtg gtagcggatc ctaccacgac tctgcaggtt aaaaacactg
    20581 gcagtctgag tgttaatcgg tatggatgga ttaacatcta gatggctatt ttaggtcaat
    20641 tcttcacccg atttccactt ttttttgaat cgtgtctcat tctgttaaaa acgtggctgg
    20701 aaattttttc tgacaatgcc ggcattctgc gtatttatat tttgcaattt tctgccattg
    20761 tggggtataa aacgcggcgc gcggcttaaa taaaaagcac acgacgtttc tttcgtgttg
    20821 ccacttccag ccgggttgaa atcagagttt tggcttgtgg gttcgtctta acaggcggcc
    20881 gtggaggtga tacgaaataa tgaaccgtag tctgctgctt aacctgtctc accgttctgg
    20941 tgaagattcg ttccccgcac tctgcatctc tgctttgcat acctgccgat gttataccca
    21001 tctcggcgct tctcaggatt caagagctgg ttacagttac tgaggactga acaagggcgc
    21061 tcttgtaaaa acaagagttt tctcgtggtt tcgccgaact gtcacactta cgttcggtta
    21121 tgtgcttaat aatgttatga aaaagaaacc ggttgcgcag ttggagcgtc agcattcact
    21181 gctggaaaat ccatgtgctt atgggttgtt atcgcagttc caggctgcga tagtcgttaa
    21241 ctgttttaca cttaataaaa taatttgagg ttcgctatgt ctgacgacat gtctatgggt
    21301 ttgccttcgt cagcgggcga acacggtgta ctacgctcca tgcaggaggt tgcaatgagc
    21361 tcccaggaag ccagcaagat gctgcgtact tacaatattg cctggtgggg caataactac
    21421 tatgacgtta acgagctggg ccacatcagc gtgtgcccgg acccggacgt cccggaagct
    21481 cgcgtcgatc tcgcgcagtt agtgaaaact cgtgaagcac agggtcagcg tctgcctgca
    21541 ctgttctgtt tcccacagat cctgcagcac cgtttgcgtt ccattaacgc cgcgttcaaa
    21601 cgtgcgaggg aatcctacgg ctataacggc gattacttcc ttgtttatcc gatcaaagtt
    21661 aaccagcacc gccgcgtgat tgagtccctg attcattcgg gcgaaccgct gggtctggaa
    21721 gccggttcca aagccgagtt gatggcagtg ctggcacatg ctggcatgac ccgtagcgtc
    21781 atcgtctgca acggttataa agaccgcgaa tatatccgcc tggcattaat tggcgagaag
    21841 atggggcaca aggtctatct ggtcattgag aagatgtcag aaatcgccat tgtgctggat
    21901 gaagcagaac gtctgaatgt cgttcctcgt ctgggcgtgc gtgcacgtct ggcttcgcag
    21961 ggctcgggta aatggcagtc ttccggcggg gaaaaatcga agttcggcct ggctgcgact
    22021 caggtactgc aactggttga aacgctgcgt gaagctgggc gtctcgacag cctgcaacta
    22081 ctgcacttcc acctcggttc gcagatggcg aatattcgcg atatcgccac gggcgttcgt
    22141 gaatccgcgc gtttctatgt ggaactgcac aagctgggcg tcaatattca gtgcttcgac
    22201 gtcggcggcg gtctgggcgt ggactatgaa ggcacccgtt cgcagtctga ctgctccgta
    22261 aactacggcc tcaatgaata tgccaacaac atcatctggg cgattggtga tgcgtgtgaa
    22321 gaaaacggtc tgccgcatcc gacggtaatc accgaatcgg gtcgtgcggt gactgcgcat
    22381 cacaccgtgc tggtgtctaa tatcatcggc gtggaacgta acgaatacac ggtgccgacc
    22441 gcgcctgcag aagatgcgcc gcgcgcgctg caaagcatgt gggaaacctg gcaggagatg
    22501 cacgaaccgg gaactcgccg ttctctgcgt gaatggttac acgacagtca gatggatctg
    22561 cacgacattc atatcggcta ctcttccggc acctttagcc tgcaagaacg tgcatgggca
    22621 gaacaactct atctgagcat gtgccatgaa gtgcagaagc aactggatcc gcaaaaccgt
    22681 gcgcatcgtc cgataatcga cgaactgcag gaacgtatgg cggacaaaat gtacgtcaat
    22741 ttctcgctgt tccagtcaat gccggacgca tgggggatcg accagttgtt cccggttctg
    22801 ccgctggaag ggctggatca agtgccggaa cgtcgcgctg tgctgctgga tattacctgt
    22861 gactctgacg gtgctatcga ccactatatt gatggtgatg gtattgccac gacaatgcca
    22921 atgccggagt acgatccaga gaatccgccg atgctcggtt tctttatggt cggcgcatat
    22981 caggagatcc tcggcaacat gcacaacctg ttcggtgata ccgaagcggt tgacgtgttc
    23041 gtcttccctg acggtagtgt agaagtggaa ctgtctgacg aaggcgatac cgttgcggat
    23101 atgctgcaat atgtgcagct cgatccgaaa acgctgttaa ctcagttccg cgatcaagtg
    23161 aagaaaaccg atcttgatgc tgaactgcaa caacagttcc ttgaagagtt cgaggcaggt
    23221 ttgtacggtt atacttatct ggaagatgag taagtccgta ttacttgaat ccgcttaatt
    23281 tagcggtgat aatccgccac aatttattgt gacaaatcca acccttcctc gtcgggccta
    23341 acgacgcgga agggtttttt tatatcgact ttgtaatagg agtccatcca tgagcacctt
    23401 aggtcatcaa tacgataact cactggtttc caacgccttt ggttttttac gcctgccgat
    23461 gaacttccag ccgtatgaca gcgatgctga ctgggtgatt actggtgtgc cgttcgatat
    23521 ggccacttct ggtcgtgcgg ggggacgcca cggtccggca gcgatccgtc aggtttcgac
    23581 gaatcttgcc tgggaacaca atcgcttccc gtggaatttc gacatgcgtg agcgcctgaa
    23641 cgtcgtggac tgcggcgatc tggtatatgc ctttggcgat gcccgtgaga tgagcgaaaa
    23701 actgcaggca cacgccgaga agctgctggc tgccggtaag cgtatgctct ctttcggtgg
    23761 tgatcacttt gttacgctgc cgctgctgcg tgctcatgcg aagcatttcg gcaaaatggc
    23821 gctggtacac tttgacgccc acaccgatac ctacgcgaac ggttgtgaat ttgaccacgg
    23881 caccatgttc tataccgcgc cgaaagaagg tctgatcgac ccgaatcatt ccgtgcagat
    23941 tggtattcgt accgagtttg ataaagacaa cggttttacc gtgctggacg cctgccaggt
    24001 gaacgatcgc agcgtggatg acgttatcgc ccaagtgaaa cagattgtgg gtgatatgcc
    24061 ggtttacctg acctttgata tcgactgcct agatcctgct ttcgcgccgg gaaccggtac
    24121 gccagtgatt ggcggcctga cctccgatcg cgccattaaa ctggtacgcg gcctgaaaga
    24181 tctcaacatt gttgggatgg acgtagtgga agtggctccg gcatacgatc agtcggaaat
    24241 caccgctctg gctgcggcga cgctggcgct ggagatgctg tatattcagg cggcgaaaaa
    24301 gggcgagtaa gcaccagatg cgatgcgcac gggtaaaacg tgccattaat gtcggatgcg
    24361 gcgtgaacgc cttatccgac ctacgttcgg cacccgtagg ccggataaga tgcgccagca
    24421 tcgcatccgg caatgcgcac aaggtaacaa atgtgccatt aatgtcagat gcggcgtgaa
    24481 cgccttatct gacctacgtt cgacaccacc aggctttact taatcccatc cgcgctcatg
    24541 cgatcgcgaa tatgctgggc gcgttcggcg gatgccggat ggtcgtcaaa cattgagctt
    24601 tggcgacctt cttccagttt cgccagtttt tcaaagctgg tggcaagacc tgccgggctg
    24661 atgccgcgtt ggcgcagaag atcgtaagaa taatcatcgg cttctgcttc ctggcgctgg
    24721 gagaattgcg aattgactaa tttctcgccc agatcaccaa gttgtgattg agataaactg
    24781 ccgacaatcc cgcccgcaga ggccgcagct actcgcacgg catttgtacc aagcgccacc
    24841 tgcatccctt tcttcacatg gcctaacgcc acgtgcccca tctcgtgacc gatcaccgct
    24901 tcgacttcgt tatccgtcat catatccatc agcccgctat agacgcggat acagccgtta
    24961 gccattgcaa aggcgttcac atccttcgcc atatacactt tgtaattcac cggctgaccg
    25021 ttgatattgt tgcctagcgc attggcaata gttgtcagac gtttagcgta ttcgctattg
    25081 gctggcgcaa tcgtcgcctt gctgtccatc tcctgacatg cctgatcgct cagggttttc
    25141 acctgcgcat cactcaaact gtaagcctga aaagcttccg ctcctgatga gagcagtccg
    25201 ttggagtcca tattctggca accagtcagt accgttgcca cgctcattgc taccaataag
    25261 gcgcgaattt tcatgttttc tcttccgcac aaggtcagga ttattctaaa aatgcgccgt
    25321 ttgcaggtga atcgacgctc agtctcagta taaggaaaag cgcagcggac gggcgagtag
    25381 attgcgcaac atgcgagcat gatccagaga tttctgaagc ggcaaaagga tgttccatgt
    25441 acatgacgcg cggcttgcgg taaattgttg gcaaattttc cggcgtagcc caaaacgcgc
    25501 tgtcgtcaag tcgttaaggg cgtgcccttc atcatccgat ctggagtcaa aatgtcctca
    25561 cgtaaagagc ttgccaatgc tattcgtgcg ctgagcatgg acgcagtaca gaaagccaaa
    25621 tccggtcacc cgggtgcccc tatgggtatg gctgacattg ccgaagtcct gtggcgtgat
    25681 ttcctgaaac acaacccgca gaatccgtcc tgggctgacc gtgaccgctt cgtgctgtcc
    25741 aacggccacg gctccatgct gatctacagc ctgctgcacc tcaccggtta cgatctgccg
    25801 atggaagaac tgaaaaactt ccgtcagctg cactctaaaa ctccgggcca cccggaagtg
    25861 ggttacaccg ctggtgtgga aaccaccacc ggtccgctgg gtcagggtat tgccaacgca
    25921 gtcggtatgg cgattgcaga aaaaacgctg gcggcgcagt ttaaccgtcc gggccacgac
    25981 attgtcgacc actacaccta cgccttcatg ggcgacggct gcatgatgga aggcatctcc
    26041 cacgaagttt gctctctggc gggtacgctg aagctgggta aactgattgc gttctacgat
    26101 gacaacggta tctctattga tggtcacgtt gaaggctggt tcaccgacga caccgcaatg
    26161 cgtttcgaag cttacggctg gcacgttatt cgcgacatcg acggtcatga cgcggcatcc
    26221 atcaaacgcg cagtagaaga agcgcgcgca gtgactgaca aaccgtccct gctgatgtgc
    26281 aaaaccatca tcggtttcgg ttccccgaac aaagccggta ctcacgactc ccacggtgcg
    26341 ccgctgggcg acgctgaaat tgccctgacc cgcgaacagc tgggctggaa atatgcaccg
    26401 ttcgaaatcc cgtctgaaat ctatgctcag tgggatgcga aagaagcagg ccaggcgaaa
    26461 gaatccgcat ggaacgagaa attcgctgct tacgcgaaag cttatccgca ggaagccgct
    26521 gaatttaccc gccgtatgaa aggcgaaatg ccgtctgact tcgacgcgaa agcaaaagag
    26581 ttcattgcta aactgcaggc taacccggcg aaaatcgcca gccgtaaagc gtctcagaat
    26641 gctatcgaag ctttcggtcc gctgttgccg gaattcctcg gcggttctgc tgacctggca
    26701 ccgtctaacc tgaccctgtg gtctggttct aaagcaatca acgaagatgc tgcgggtaac
    26761 tacatccact acggtgttcg cgagttcggt atgaccgcga ttgctaacgg tatctccctg
    26821 cacggtggtt tcctgccgta cacctccacc ttcctgatgt tcgtggaata cgcacgtaac
    26881 gccgtacgta tggctgcgct gatgaaacag cgtcaggtga tggtttacac ccacgactcc
    26941 atcggtctgg gcgaagatgg cccgactcac cagccggtag agcaggtcgc ttctctgcgc
    27001 gtaaccccga acatgtctac atggcgtccg tgtgaccagg ttgaatccgc ggtcgcgtgg
    27061 aaatacggtg ttgagcgtca ggacggcccg accgcactga tcctctcccg tcagaacctg
    27121 gcgcagcagg aacgaactga agagcaactg gcaaacatcg cgcgcggtgg ttatgtgctg
    27181 aaagactgcg ccggtcagcc ggaactgatt ttcatcgcta ccggttcaga agttgaactg
    27241 gctgttgctg cctacgaaaa actgactgcc gaaggcgtga aagcgcgcgt ggtgtccatg
    27301 ccgtctaccg acgcatttga caagcaggat gctgcttacc gtgaatccgt actgccgaaa
    27361 gcggttactg cacgcgttgc ggtagaagcg ggtattgctg actactggta caagtatgtt
    27421 ggcctgaatg gcgctatcat cggtatgacc accttcggtg agtcagctcc ggcagagttg
    27481 ctgttcgaag agttcggctt caccgtcgac aacgtcgttg cgaaagcaaa agaactgctg
    27541 taattagcat ttcgggtaaa aaggtcgctt cggcgacctt ttttattacc ttgatatgtc
    27601 cgtttgcgga caagcaatag ataaagcgtg ttgcagatca caaatattta tatgcaataa
    27661 atatcaatta tgtaatatgc atcacgatat gcgtattgac atttgttgtt atatctataa
    27721 ctcaatgtta tataagaaat taataattca ctgttttcaa aacaccggtt tccctgctca
    27781 attgctttca ttaaaccgca cgagtatttt atttttaccg ccggacggta ccctaaaaat
    27841 aaaataagga taatttatgc ggcttagtga ctattttcca gaatcatcaa tctcagttat
    27901 acattcagca aaagattggc aggaagctat cgatttctcg atggtatcat tgctggataa
    27961 aaactatatc agcgagaatt acattcaggc aattaaagat tccaccatta acaatggccc
    28021 ttattatatt ctcgcaccag gcgtggcaat gcctcatgcg cgaccggaat gtggggcgct
    28081 taaaaccggg atgtctttga cattacttga acaaggtgtt tattttccgg ggaatgacga
    28141 accaattaaa ttactcatcg gactctctgc tgccgatgcc gattcgcaca ttggcgctat
    28201 tcaggcgtta agtgaattac tgtgcgaaga agaaatactc gaacaactct taacagcatc
    28261 atcagaaaaa caattagcgg acattatcag ccacagataa ttactctctc ctctacagga
    28321 taatattatg gaaaacaagt ctgctcgtgc aaaggtccag gcttttgggg gctttttgac
    28381 tgcaatggtc atccccaata ttggtgcttt tattgcctgg ggttttatta ctgcgttatt
    28441 tattcccacc ggttggctgc ctaacgaaca tttcgccaaa attgtcggcc cgatgattac
    28501 ctatttattg cccgtgatga ttggttctac aggtggtcat ctggtcggcg gtaaacgcgg
    28561 ggcggtcatg ggcggaatag gtactattgg tgtgatcgtt ggcgcagaga tcccgatgtt
    28621 ccttggctca atgatcatgg ggccgctcgg tgggttggtc ataaaatatg tcgataaggc
    28681 actggaaaaa cgcatacctg ccggttttga gatggttatc aataacttct cattaggtat
    28741 cgcggggatg ctcctttgtc tgctgggttt tgaagtgatc ggcccggcgg tgttaattgc
    28801 caatactttc gtcaaagagt gtatcgaggc gctggtacat gcgggttatc tgcctctgct
    28861 gtcagtcatc aatgaaccgg cgaaagtgct tttccttaat aatgcgatcg atcagggcgt
    28921 ctattatccg ctgggaatgc aacaggcttc ggttaacggt aaatccatct tctttatggt
    28981 ggcctctaac ccaggtccgg gcctggggct gctgctggcg tttaccttgt ttggtaaagg
    29041 gatgagtaaa cgttctgcgc ccggggcgat gattattcac ttcctcggtg ggatccacga
    29101 actgtatttc ccatatgtgc tgatgaagcc gctgactatt attgccatga ttgcgggcgg
    29161 tatgtctggt acctggatgt ttaacttact ggacggtggt ctggtggctg gcccaagtct
    29221 ggggtctatc tttgcttacc tggcactgac gccgaaaggc tcgttcctgg cgacaattgc
    29281 cggtgttacg gtaggtaccc tggtgtcctt tgctattact tcgctgatac tgaagatgga
    29341 aaaaacggtg gaaacggaga gcgaagatga gtttgctcag tcaaccaatg cggttaaggc
    29401 gatgaaacaa gagggtgcat tctcgttaag cagggttaag cgtatcgcct ttgtttgcga
    29461 tacggggatg ggctccagtg cgatgggcgc gaccaccttc cgtaaacgcc tggaaaaagc
    29521 ggggctggca atagaagtaa aacattacgc gatagaaaac gtgcctgcgg atgccgatat
    29581 cgtcgtcact cacgccagtc tggaagggcg cgtgaaacgt gtgacggata aaccgctgat
    29641 attgattaat aactatattg gcgatccaaa actcgacact ttatttaatc aattaaccgc
    29701 cgaacataaa cactgattac agaggtaaaa atgaaaacca aagttgctgc tatttatggc
    29761 aagcgggatg tccgtctgcg cgaatttgaa ctgccagaaa ttaccgataa tgaactgtta
    29821 gtgagtgtaa tttctgacag cgtctgttta tcgacctgga aagcggcgtt actcggtagt
    29881 gaacataaac gcgtacccga cgatttagaa aatcatccgg tcattaccgg gcatgaatgt
    29941 gccggggtta ttgtcgaagt gggtaaaaat ctcactggca aatataaaaa aggccagcgt
    30001 tttgtattgc aaccggcgat ggggttacca agcggatatt cagcaggcta cagctacgaa
    30061 tattttggcg gtaacgccac ctatatgatt attcccgaaa tcgccattaa tttgggctgc
    30121 gtattaccgt atcacggctc ttattttgct gcggcgtcgc tggtagagcc tatgtgctgc
    30181 attattggtg cttatcatgc caattatcac accacgcaat atgtttatga gcatcgcatg
    30241 ggcgtcaaac ctggcggcaa tattgcactg ctggcgtgtg cgggtccgat gggcattggc
    30301 gctatcgatt acgccattaa cggtggcata caaccgtcac gggtggtggt ggtcgatatc
    30361 gacgacaaac gcctggcgca ggtgcagaag ctgctgccgg tggatctggc ggccagtaaa
    30421 ggcattgagc tggtgtatgt gaataccaaa gggatgagcg atccggtcca gacgctgcgg
    30481 gcgctgacag gagatgtcgg gttcgatgac atttttgttt atgcggcggt gcctgctgtc
    30541 gttgagatgg ctgacgaatt actggcggaa gatggctgtc tgaacttctt tgccgggccg
    30601 acggataaaa acttcaaagt gccgtttaat ttctacaacg tccattacaa cagcacgcac
    30661 gtagtcggta catccggcgg ttcaacggac gacatgaaag aggcgattgc gttaagcgcc
    30721 acggggcagt tgcagccgtc ctttatggtc acgcatatcg gtgggctgga tgcggtgcca
    30781 gataccgtgc tcaatctgcc ggatatccct ggcggtaaaa aactcattta taacggcgtg
    30841 accatgccgc tcactgccat tgccgatttt gccgaaaaag gcaaaaccga tccgctattt
    30901 aaagagttgg cgcggctggt tgaggaaacg cacggcatct ggaatgaaca ggccgagaaa
    30961 tatctgctgg cacaatttgg cattgatatc ggggaggccg cgcaatgatg tccctggcgt
    31021 ggccattatt tcgcgttacg gaacaggcag cgttagctgc ctggccgcaa accggatgtg
    31081 gcgacaaaaa taaaattgat ggcctggcgg tcaccgcgat gcgccaggca ttaaacgacg
    31141 tcgcttttcg tggacgagtg gttatcggcg aaggagaaat tgaccatgcg ccgatgctgt
    31201 ggattggcga agaggtgggc aaaggcgatg ggccagaagt cgatatcgcg gttgatccca
    31261 ttgaaggcac gcggatggtg gcgatggggc agagcaatgc gctggcggtc atggctttcg
    31321 cccctcggga tagcctgtta catgcgcccg atatgtatat gaaaaagctg gttgttaatc
    31381 gactggctgc cggggtgatt gatctgtctt tgcctctggc ggacaacttg cgtaacgtgg
    31441 cgcgggcgtt aggtaaaccg ctggacaagc tgcgcatggt tacgctggat aaaccgcgcc
    31501 tgagtgccgc gattgaggaa gcaacacagc tgggcgtgaa ggtttttgcc ctgccggatg
    31561 gcgatgtcgc cgccagcgtg ctgacctgct ggcaggataa tccttacgat gtgatgtaca
    31621 ccatcggcgg tgcgccagaa ggcgtgattt ctgcctgtgc cgttaaagca ttaggcgggg
    31681 atatgcaggc cgagctgatt gacttttgtc aggctaaagg ggattacacg gaaaatcggc
    31741 agattgcaga gcaggagagc aagcgttgca aggcaatggg cgttgacgtt aaccgtgtgt
    31801 actcgctcga tgaactggtg agggggaacg atatcctctt tagcgctacg ggcgtgacgg
    31861 gcggcgagtt ggtgaacggt atccaacaga cggcgaatgg ggtgcggacg cagacattac
    31921 tgatcggcgg cgcggaccaa acgtgtaata taatagactc cctgcattga tggtgatcta
    31981 tcggaagacc aatggcgaag ctgacagaag atgatgtgct tgagcaactg gatgcacagg
    32041 acaatttatt ttcatttatg aaaactgcgc attctatttt gctccagggg atacgccagt
    32101 ttctgccgtc gctgtttgtc gataacgatg aagagatcgt ggaatatgca gtgaagccgt
    32161 tactcgccca aagcggcccg cttgacgata ttgatgttgc gctgcgtttg atttatgcgc
    32221 tggggaaaat ggataaatgg ctgtacgccg atatcacgca tttttcccag ttctggcatt
    32281 acctgaacga acaggatgaa acgcccggat ttgccgatga catgacctgg gattttatca
    32341 gcaacgtcaa tagcataacc cgcaatgcga tgctctatga cgcgttgaaa gcgatgaagt
    32401 tcgccgattt cgccgtctgg tcagaggcgc gttttagcgg gatggtcaaa acggcgctga
    32461 cgctggcagt aacgacaatc ttaaaggaat taacgccgtg aaaattgaat taacggtgaa
    32521 tgggctgaag atacaggcac agtaccagaa cgaagagatt gaaaatgtac ataaaccgtt
    32581 gctgcgtatg ctggcggcct tacagacggt aaatccgcag cggcgtacgg tggtttttct
    32641 ttgcgctccg ccggggacgg gtaaatctac cctaaccacc ttctgggaat atctcgcgca
    32701 acaagacccg gagctgcccg ctattcagac gctcccgatg gatggttttc accattacaa
    32761 tagctggctg gacgcgcatc aattgcgccc cttcaaaggc gcaccagaga catttgacgt
    32821 tgcgaaactg gcggaaaatc tgcgccaggt ggtggaaggg gattgtacgt ggccgcagta
    32881 cgatcgacaa aagcatgatc ctgttgaaga tgcgttgcac gttaccgcac cactcgtcat
    32941 cgtcgaagga aactggttgt tgctggacga cgagaagtgg tgccagcttg cgcaattttg
    33001 tgatttctcg atctttatca acgcgccagc tactgcgttg cgggagcgtc tggtggggcg
    33061 caagctggcg ggaggggtat cgctggcaga tgcagaagcc ttttatgacc gtaccgacgg
    33121 gccgaatgtt cgccgggtgc tggaagagag tctgcctgca aatttgacct taatgatgac
    33181 cgctacgggt gaatatcgtc tggtggatta acgttcctga ttacaccatc ttccagtcgc
    33241 accacacgag agaaatgacg ccgggtaaat gcggcgtcat gactgattgc taccacgctc
    33301 gtgcctcgct ggcggcactt ctccagccag ctttcaaaca cgctcaacca attttcgtca
    33361 aaatcccgac tcggctcgtc cagcagtaat agtggcggag agagtgcttc caggcaggcg
    33421 acggcgacca tccgtcgctg cgcactatgc agatccaacg ggtgcgagtt ggcagtgtct
    33481 gctaactgac aacattgcag ggcagcatgg gtacgttgag tgatttcatc cgccgggcat
    33541 ttttgcagtt tcaaaccaaa tgcgatctcg tcagcgacgg tgctgtgaaa aagttggttt
    33601 tccgcttcct gaaacagtac gccaaccttt gccgcgcgct ggcggttttt caggtttttc
    33661 atcgcctgtt gttgcagcat aaccgtaccg gctgtgggcg taagaagacc tgccattact
    33721 cgtaacagcg tcgatttccc tgcgccattg tcaccggtca gcgccaacca ttcaccttgt
    33781 ttgagttgca gagaaatgtc gcaaagacaa tccgttgccg cgccaggcca gcgataagat
    33841 atctgattta acgttaacat agtggctgga gcgctccgtt ttgcaattgc cacgcttgct
    33901 ggcagtagtt caggaaaggt gtgcgatgac gttcaaaaag aataatcagc gaaccacgtt
    33961 caaacgccca gtgttgtaag cgctgcaaca gcatttcgct ggcctgcggc gtcaggcggc
    34021 taaaggcttc atcaagaatt aagagctttg gctgcatcgc gatggcgcag gcgatgacca
    34081 cgcgctgtgt ctctccaccg gagagtgttg ccgggtgacg atggcgcaac ggctggcact
    34141 cggtcagagt cagggcggca tcaatccgcg ccataatctc tgcttcagca agacacagat
    34201 tttctggccc aaaggcaact tcttcttcca cgctaaaggt acagccggag agctgcaaat
    34261 agggcgattg ctggacgagc tggatggtgg cggattgttc attaagcggg agatgcccaa
    34321 tgggcgtacc gaggagcgtc cccgtgcctg ttatctctcc tggcagaaag tccggatacc
    34381 agcccgccat cagctgtgcc agcgtacttt tgccactgcc gttgtcacca aagatggcga
    34441 ccatccccgg tttgacatag tgaaagtcat aacagaacgg aggatgcgtt gagtgtgtcg
    34501 ggcaataacg gaactgttct aacgtaacca tatccacgct ccgaattctg tcagcatcag
    34561 caagatcatg gtgtaacgcg ctacgcgttg tagggtgcta tcggctggtg cccacaacgt
    34621 tgttcgtcgg ttgtggatcc gaaatgcccg catatccagc gccgcaccac ggacagccag
    34681 atcgttaagc gcattgtgtg ttagcggaat aattaacgcg ggcatcgcac gtaaacgctg
    34741 ataccagccc tcatccagcg gcacgccgcg ggcacgctgc gcttcatgaa taatcgctaa
    34801 ttgccgtttt aactgctcaa cgaccaataa cggcccggca aacagataag cgacgcctgg
    34861 cggcaggcga gaagcaaaca gagcgcgaat aaaacattgc acggggacgt actgcatcca
    34921 caactgtgag gttgaaacaa tcaccagaat ccgcaaccag agcgtaatag catgcgacca
    34981 ccgttctggc gagcgtggcg tgccgcttag ccattctggt agccagccac cgtgtaccag
    35041 ccacagcccg gctcccagcg agaacatcag ccagacgaca tattttgccc gccgccgcgt
    35101 ggcctttagc gcaataaggc agaaaaaggt cgccgcgcta tagatcggca aaatggtctg
    35161 cgcgggcagg ataagcgtgg tgcaggccgc caatgcccac agtgttaatg aggtaaatgg
    35221 atgcatttag cgcacggcag ccattgccgg aaaatggcgc gtggtgcgaa tcggcagctg
    35281 gcgcagtaaa agccagacaa tcaccgctgt aagaatttta tcgaccaggt tcgcgcctat
    35341 caccgtaatc gccaccgact ccaccaggtt ttgccccatc gagtgcatcc aggcaacgaa
    35401 caaatcggct ccgctaccgg tgacaccgcc gaacagcgca gtacgcagcg gtacagcgac
    35461 gaccgttacc gcaagcgtaa taatcacgcc gctgacaacg acttttggca gggtgcgaaa
    35521 ccaacccgct cgcgccagcc aaccggcgac taggccgatg accatcgcga cgggtgcaaa
    35581 tgcggcggcg atcgggtcgg tcagcaatcc ccagagtaaa ttggtcagta agccggtcag
    35641 cattccgatc accggaccaa gcagtaccgc gctaataagt gtgccgatgg agtcgagaaa
    35701 tataggtaat ttgaccatgc tggccagttg cccgccgatc atattgatgg cgatagatat
    35761 aacgatcaac accagtgcct gactggaaaa gtggctacgc gccataagaa aacctctttg
    35821 aaagtagtta tttaacgggg gaaatatgca actcgtcgta accagaaagc gcgcaaggtt
    35881 ggcggctaaa cgccagttcc tcaaggctac cgatcaccag ttcaagcgtg gtccgcacgg
    35941 tacagccggg catcatatga ccgccaagca tcgtgccgtg tggatcggaa acgcagagat
    36001 gaagatgctc accactctgt tccagcgtgc cgtttagtga gatcacttcg aatgtaccgt
    36061 tcaggagcgt ggtgccttct tgcctggcat agcgcaaggc gacgtcagtc aggctaccgg
    36121 tacagcctgc aatccaggcg gcatgaagtt gatgctgctg tgcaaaagcg tgtagctggg
    36181 aaaaaacttc ctgcccgggg aggaggcgca gtgcgtaaaa gcgtgcggag gaagaatatg
    36241 ggtagggcaa tgtcatcatt ggagtactcc gaaactcgga cgtattgcgg ataatatcga
    36301 cgctgcatca gtcatattct gatataagtc agggtttgtg acggcacaac gaaaagattt
    36361 tgctacgctt attcgtgatt ctgcaaagtg gaataaattc tacaggggcg gggctgaaaa
    36421 cgtgacgtaa gtcagataat tgctttcttt gactggacaa tcattccttt tattccacgt
    36481 ttcgcttatc ctagctgaag cgtttcagtc gattaaatgt tcgacaatta accaatcagt
    36541 cgcagtttgc gacaggtaag gtttccccgg acgatttgct ggattactct gtctggcaaa
    36601 tttgctcagg gaaaaccttg caggagatct atgaccgtac gcgtagcgat aaatggcttc
    36661 ggtcgcatcg ggcgtaatgt ggttcgtgct ttgtatgaat ccggacgccg ggcggaaatt
    36721 accgtggtgg caatcaacga actggcggat gctgcgggca tggcgcattt gttgaaatat
    36781 gacaccagcc atggccgttt tgcatgggaa gtacgacagg aacgcgatca actttttgtt
    36841 ggtgatgacg ccatccgcgt attgcatgaa cgttcactgc aatcgctccc ctggcgtgaa
    36901 cttggcgttg atgtagtcct cgactgcacc ggcgtatatg gctcccgcga gcatggcgaa
    36961 gcgcatattg ccgccggggc caaaaaagtg ctcttttcac atcctggcag taacgatctc
    37021 gacgcgaccg ttgtttacgg cgtcaatcag gatcaacttc gtgcggaaca ccgcatcgtt
    37081 tctaacgctt cctgtaccac gaattgcata attcccgtca tcaaattgtt agatgatgcg
    37141 tacggtattg agtccggcac tgtgaccaca attcactccg ccatgcacga tcaacaggtt
    37201 attgatgcat accatcctga cctgcgtcgc acccgggcag ccagccagtc gatcattccg
    37261 gtcgatacta aactggccgc cggtatcaca cgattttttc cgcaatttaa cgatcgcttt
    37321 gaagcgattg cggtacgtgt gccaaccata aatgtgacgg caatcgattt aagcgtgacg
    37381 gtgaagaaac ctgtaaaagc caatgaagtc aacctgttgc tgcaaaaagc agcacaaggt
    37441 gcatttcatg gtatagttga ctatacggaa ttgccgttgg tctctgtaga ttttaaccac
    37501 gatccgcaca gtgccattgt cgatggcacc caaacccggg tcagtggcgc acacctgatc
    37561 aaaacgttgg tctggtgcga taacgaatgg ggctttgcta accgaatgct cgacacgacg
    37621 ttagctatgg ctactgttgc tttcaggtaa gacgcaagca gcgtctgcaa aacttttaga
    37681 atcaacgaga ggattcacca tgtctgtaat taagatgacc gatctggatc ttgctgggaa
    37741 acgtgtattt atccgtgcgg atctgaacgt accagtaaaa gacgggaaag taaccagcga
    37801 cgcgcgtatc cgtgcttctc tgccgaccat cgaactggcc ctgaaacaag gcgcaaaagt
    37861 gatggtaact tcccacctgg gtcgtcctac cgaaggcgag tacaacgaag aattctctct
    37921 gctgccggtt gttaactacc tgaaagacaa actgtctaac ccggttcgtc tggttaaaga
    37981 ttacctcgac ggcgttgacg ttgctgaagg tgaactggtt gttctggaaa acgttcgctt
    38041 caacaaaggc gagaagaaag acgacgaaac cctgtccaaa aaatacgctg cactgtgtga
    38101 cgtgttcgta atggacgcat tcggtactgc tcaccgcgcg caggcttcta ctcacggtat
    38161 cggtaaattc gctgacgttg cgtgcgcagg cccgctgctg gcagctgaac tggacgcgct
    38221 gggtaaagca ctgaaagaac ctgctcgccc gatggtggct atcgttggtg gttctaaagt
    38281 atctaccaaa ctgaccgttt tggactccct gtctaaaatc gctgaccagc tgattgttgg
    38341 tggtggtatc gctaacacct ttatcgcggc acaaggccac gatgtgggta aatccctgta
    38401 cgaagctgac ctggttgacg aagctaaacg tctgctgacc acctgcaaca tcccggttcc
    38461 gtctgatgtt cgcgtagcaa ccgagttctc tgaaactgca ccggctaccc tgaaatctgt
    38521 taacgatgtg aaagctgacg agcagatcct ggatatcggt gatgcttccg ctcaggaact
    38581 ggctgaaatc ctgaagaatg cgaaaaccat tctgtggaac ggtccggttg gcgtgttcga
    38641 attcccgaac ttccgcaaag gtactgaaat cgtggctaac gctatcgcag acagcgaagc
    38701 gttctccatc gctggcggcg gcgacactct ggcagcaatc gacctgttcg gcattgctga
    38761 caaaatctcc tacatctcca ctggcggcgg cgcattcctc gaattcgtgg aaggtaaagt
    38821 actgcctgca gtagcgatgc tcgaagagcg cgctaagaag taaaaaatca cagggcaggg
    38881 aaacctgccc ttgtttcagc gcgcttttag agcacgcatt ttttcatact ctaaataatt
    38941 cgagttgcag gaaggcgaca agagagtgaa tccccaggag cttacataag taagtgactg
    39001 gggtgagcga acgcagacgc agcacatgca acttgaagta tgatgagtat aaggcccgac
    39061 gatacaggac aagagacatg tctaagattt ttgatttcgt aaaacctggc gtaatcactg
    39121 gtgatgacgt acagaaagtt ttccaggtag caaaagaaaa caacttcgca ctgccagcag
    39181 taaactgcgt cggtactgac tccatcaacg ccgtactgga aactgctgct aaagttaaag
    39241 cgccggttat cgttcagttc tccaacggtg gtgcttcctt tatcgctggt aaaggcgtga
    39301 aatctgacgt tccgcagggt gctgctatcc tgggcgcgat ctctggtgcg catcacgttc
    39361 accagatggc tgaacattat ggtgttccgg ttatcctgca cactgaccac tgcgcgaaga
    39421 aactgctgcc gtggatcgac ggtctgctgg acgcgggtga aaaacacttc gcagctaccg
    39481 gtaagccgct gttctcttct cacatgatcg acctgtctga agaatctctg caagagaaca
    39541 tcgaaatctg ctctaaatac ctggagcgta tgtccaaaat cggcatgact ctggaaatcg
    39601 aactgggttg caccggtggt gaagaagacg gcgtggacaa cagccacatg gacgcttctg
    39661 cactgtacac ccagccggaa gacgttgatt acgcatacac cgagctgagc aaaatcagcc
    39721 cgcgtttcac catcgctgcg tccttcggta acgtacacgg tgtttacaag ccgggtaacg
    39781 tggttctgac tccgaccatc ctgcgtgatt ctcaggaata tgtttctaag aaacacaacc
    39841 tgccgcacaa cagcctgagc ttcgtattcc acggtggttc cggttctact gctcaggaaa
    39901 tcaaagactc cgtaagctac ggcgtagtga aaatgaacat cgataccgat acccaatggg
    39961 caacctggga aggcgttctg aactactaca aagcgaacga agcttatctg cagggtcagc
    40021 tgggtaaccc gaaaggcgaa gatcagccga acaagaaata ctacgatccg cgcgtatggc
    40081 tgcgtgccgg tcagacttcg atgatcgctc gtctggaaaa agcattccag gaactgaacg
    40141 cgatcgacgt tctgtaagat attcctttct gcttatctca aggcccgctc tgcgggtctt
    40201 tttttcgcca aaagcaaaat tgcctgatgc gctacgctta tcaggcctac acgtatcctg
    40261 caatatattg aatttgcagt tttttgtagg ccggataagg cgttcacgcc gcatccggca
    40321 tgacaaagcg cacattgata tctgtctgag ccccgctctg cggggatttt ttcgccaaaa
    40381 gcaaggtgat tcagatgaga aatctgtgac ctatttggca aaattatgct ttattgttta
    40441 cccttgtcag actgcccgtc ataaggcggc ggagtgtatt tctccatttt gagtcagttg
    40501 aaaaggaata ttgaatggaa gatttgaatg ttgtcgatag cataaacggc gcgggaagct
    40561 ggctggtagc taaccaggcg ctgctgctaa gttatgcagt aaacatcgtg gcggcactcg
    40621 cgatcatcat cgttggtttg attatcgcgc ggatgatttc caacgcggtg aatcgcctga
    40681 tgatctcccg taaaatcgat gccactgttg ctgattttct ttctgcatta gtccgttacg
    40741 gtattatcgc ctttacgcta atcgctgccc tgggacgcgt gggtgtacaa acagcgtcag
    40801 tcattgctgt actcggtgcc gcaggcttag ctgttggtct ggctttgcag gggtcacttt
    40861 ctaacctggc cgctggcgtg ttacttgtca tgttccgacc gttccgtgcc ggagaatatg
    40921 ttgacctcgg cggcgtagcc ggtactgtgc tgagtgtgca gattttctcc accaccatgc
    40981 gtactgcaga cggtaaaatt atcgttattc cgaacggtaa aattattgcc ggaaatatta
    41041 ttaacttctc ccgcgagcca gttcgccgta acgaatttat tattggcgtg gcgtatgatt
    41101 ccgatatcga tcaggttaag cagatcctga ccaatattat ccagtctgaa gatcgcattt
    41161 tgaaagatcg cgaaatgact gtgcgcctga acgaacttgg tgcatcgtcg attaatttcg
    41221 tggtccgcgt ctggagcaac agcggcgatc tgcaaaacgt gtactgggat gtgctggagc
    41281 gtattaaacg tgaatttgat gccgccggta tcagcttccc gtacccgcaa atggatgtga
    41341 actttaagcg ggtgaaagaa gacaaagctg cgtaatcaac gctgaggcca gataatactc
    41401 tctggcctct ctcttattag ttattctgat tgccaattaa tattatcaat ttccgctaat
    41461 aacaatcccg cgatatagtc tctgcatcag atacttaatt cggaatatcc aacgtgtttt
    41521 cttattactt tcaaggtctt gatcttgggg cagctatgat tctgccgctc ggcccacaaa
    41581 atgcttttgt gatgaatcag ggcattcgtc gtcagtacca cattatgatt gccttacttt
    41641 gcgctatcag cgatttggtc ctgatttgcg cggggatttt tggtggtagc gcgttattga
    41701 tgcagtcgcc gtggttgctg gcgctggtca cctggggcgg cgtagtcttc ttgctctggt
    41761 atggttttgg cgcttttaaa acagcgatga gtagtaatat tgagttagct agtgccgaag
    41821 tcctgaagca aggcagatgg aaaattatcg ccaccatgtt ggcagtgacc tggctgaatc
    41881 cgcatgttta cctggatact tttgttgtac tgggcagcct tggcgggcag cttgacgtcg
    41941 aaccaaaacg ttggtttgcg ctcgggacaa ttagcgcctc tttcctgtgg ttctttggtc
    42001 tggctattct cgcagcctgg ctggcaccgc gcctgcgcac ggcaaaatca cagcgcatta
    42061 tcaatctggt agtgggatgt gtcatgtggt ttatagcctt gcagctggcg agagacggta
    42121 ttgctcatgc acaagccttg ttcagttagg cgctatctga tggaaaaata aaacagaggc
    42181 gctaagcttg cctccagagg tcctgaattt tgggcaagtc aattaacacg gagagactaa
    42241 cgtgaagttc aaagttatcg ccctagcggc attaatgggt attagcggga tggcagcgca
    42301 ggctaacgaa ttgccggatg gaccgcatat tgtcacctcc ggtacggcaa gtgtggatgc
    42361 ggtgccagac attgccactc ttgcgattga agttaacgtg gccgcgaagg atgccgctac
    42421 agccaagaaa caggcagatg agcgcgtcgc acaatacatt tccttccttg aactcaatca
    42481 gatcgcgaaa aaagatatca gctcagcgaa tttacgcacc cagccagatt atgattatca
    42541 ggatggtaaa agtatcctga aaggctaccg cgctgtgaga acggtggaag tcacgctccg
    42601 tcagttagac aaactgaatt ccttgctgga tggcgcgctg aaggcgggtc ttaacgaaat
    42661 tcgttcagtg tcgctgggcg tggcgcagcc ggatgcctat aaagacaaag cgcgtaaggc
    42721 agcgattgat aacgcgattc atcaggcgca ggaactggcg aacggctttc atcgtaaact
    42781 ggggccggta tatagcgtgc gctaccatgt ttccaactat cagcccagcc caatggtgcg
    42841 gatgatgaaa gccgatgccg cgccggtgtc tgcccaggaa acttacgagc aggccgctat
    42901 tcagtttgat gatcaggtcg atgtggtctt ccagttagaa cctgtggatc aacaacccgc
    42961 taaaacacct gcagcacaat aatacttgta gcccgacgta ttcgtcgggc taatgccttt
    43021 acccgatatt gcctcctttt tctgtaattt tctgtaaaac catgacgtaa tcacctatct
    43081 ttgctcgcgg tttcatcaag gataggctga ctgaaacggt taagcggaca aggatgcggg
    43141 g
//
LOCUS       DADLFA010000008        42395 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000008 DADLFA010000000
VERSION     DADLFA010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42395
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.008"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            100..639
                     /locus_tag="JQH65_000406"
     CDS             100..639
                     /locus_tag="JQH65_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6137925.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQVAANKRAATAAANKKARQDKLDAEASADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     NLMKSVGKAEENKSDSWFN"
     gene            complement(734..2311)
                     /gene="guaA"
                     /locus_tag="JQH65_000407"
     CDS             complement(734..2311)
                     /gene="guaA"
                     /locus_tag="JQH65_000407"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAY6137926.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMHMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYNKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2380..3846)
                     /gene="guaB"
                     /locus_tag="JQH65_000408"
     CDS             complement(2380..3846)
                     /gene="guaB"
                     /locus_tag="JQH65_000408"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004159092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAY6137927.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4008..5378
                     /gene="xseA"
                     /locus_tag="JQH65_000409"
     CDS             4008..5378
                     /gene="xseA"
                     /locus_tag="JQH65_000409"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAY6137928.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5375..5590)
                     /locus_tag="JQH65_000410"
     CDS             complement(5375..5590)
                     /locus_tag="JQH65_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6137929.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(5659..7131)
                     /gene="der"
                     /locus_tag="JQH65_000411"
     CDS             complement(5659..7131)
                     /gene="der"
                     /locus_tag="JQH65_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAY6137930.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEVEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7249..8427)
                     /gene="bamB"
                     /locus_tag="JQH65_000412"
     CDS             complement(7249..8427)
                     /gene="bamB"
                     /locus_tag="JQH65_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HAY6137931.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYD
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8438..9058)
                     /locus_tag="JQH65_000413"
     CDS             complement(8438..9058)
                     /locus_tag="JQH65_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HAY6137932.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9076..10350)
                     /gene="hisS"
                     /locus_tag="JQH65_000414"
     CDS             complement(9076..10350)
                     /gene="hisS"
                     /locus_tag="JQH65_000414"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAY6137933.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDLNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPIVDIYLV
                     ASGADTQSAAMALAERLRDELQGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     TNGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10461..11579)
                     /gene="ispG"
                     /locus_tag="JQH65_000415"
                     /gene_synonym="gcpE"
     CDS             complement(10461..11579)
                     /gene="ispG"
                     /locus_tag="JQH65_000415"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HAY6137934.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(11606..12619)
                     /gene="rodZ"
                     /locus_tag="JQH65_000416"
     CDS             complement(11606..12619)
                     /gene="rodZ"
                     /locus_tag="JQH65_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HAY6137935.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLLGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTAATTPDGAAPLPTDQAGVTTPVADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(12904..14058)
                     /gene="trmG/rlmN"
                     /locus_tag="JQH65_000417"
     CDS             complement(12904..14058)
                     /gene="trmG/rlmN"
                     /locus_tag="JQH65_000417"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HAY6137936.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14208..14639)
                     /gene="ndk"
                     /locus_tag="JQH65_000418"
     CDS             complement(14208..14639)
                     /gene="ndk"
                     /locus_tag="JQH65_000418"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAY6137937.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(14788..17100)
                     /gene="pbpC"
                     /locus_tag="JQH65_000419"
     CDS             complement(14788..17100)
                     /gene="pbpC"
                     /locus_tag="JQH65_000419"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HAY6137938.1"
                     /translation="MLRLLTKRGCWITLAAVPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKILQLWRALQLEWHLSKREILILY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLEWPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDCTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTSCP
                     PYGHYAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(17101..22062)
                     /gene="yfhM"
                     /locus_tag="JQH65_000420"
                     /pseudo
     CDS             complement(17101..22062)
                     /gene="yfhM"
                     /locus_tag="JQH65_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
     gene            22262..23107
                     /gene="sseA"
                     /locus_tag="JQH65_000421"
     CDS             22262..23107
                     /gene="sseA"
                     /locus_tag="JQH65_000421"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HAY6137939.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPKTFAVAMRELGVNLDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            complement(23600..24376)
                     /gene="sseB"
                     /locus_tag="JQH65_000422"
     CDS             complement(23600..24376)
                     /gene="sseB"
                     /locus_tag="JQH65_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HAY6137940.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLFIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(24518..25801)
                     /gene="pepB"
                     /locus_tag="JQH65_000423"
     CDS             complement(24518..25801)
                     /gene="pepB"
                     /locus_tag="JQH65_000423"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HAY6137941.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRASERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(25979..26179)
                     /gene="iscX"
                     /locus_tag="JQH65_000424"
     CDS             complement(25979..26179)
                     /gene="iscX"
                     /locus_tag="JQH65_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HAY6137942.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(26191..26526)
                     /gene="fdx"
                     /locus_tag="JQH65_000425"
     CDS             complement(26191..26526)
                     /gene="fdx"
                     /locus_tag="JQH65_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HAY6137943.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(26528..28378)
                     /gene="hscA"
                     /locus_tag="JQH65_000426"
     CDS             complement(26528..28378)
                     /gene="hscA"
                     /locus_tag="JQH65_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HAY6137944.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(28395..28910)
                     /gene="hscB"
                     /locus_tag="JQH65_000427"
     CDS             complement(28395..28910)
                     /gene="hscB"
                     /locus_tag="JQH65_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HAY6137945.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(29006..29329)
                     /gene="iscA"
                     /locus_tag="JQH65_000428"
     CDS             complement(29006..29329)
                     /gene="iscA"
                     /locus_tag="JQH65_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HAY6137946.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(29346..29732)
                     /gene="iscU"
                     /locus_tag="JQH65_000429"
     CDS             complement(29346..29732)
                     /gene="iscU"
                     /locus_tag="JQH65_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HAY6137947.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLVEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(29760..30974)
                     /gene="iscS"
                     /locus_tag="JQH65_000430"
     CDS             complement(29760..30974)
                     /gene="iscS"
                     /locus_tag="JQH65_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAY6137948.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(31086..31574)
                     /gene="iscR"
                     /locus_tag="JQH65_000431"
     CDS             complement(31086..31574)
                     /gene="iscR"
                     /locus_tag="JQH65_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HAY6137949.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(31844..32584)
                     /gene="trmJ"
                     /locus_tag="JQH65_000432"
     CDS             complement(31844..32584)
                     /gene="trmJ"
                     /locus_tag="JQH65_000432"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HAY6137950.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            32703..33506
                     /gene="suhB"
                     /locus_tag="JQH65_000433"
     CDS             32703..33506
                     /gene="suhB"
                     /locus_tag="JQH65_000433"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HAY6137951.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            33651..34505
                     /locus_tag="JQH65_000434"
     CDS             33651..34505
                     /locus_tag="JQH65_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY6137952.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQDDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKETKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            34696..35976
                     /gene="csiE"
                     /locus_tag="JQH65_000435"
     CDS             34696..35976
                     /gene="csiE"
                     /locus_tag="JQH65_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HAY6137953.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFAKLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(35968..37107)
                     /locus_tag="JQH65_000436"
     CDS             complement(35968..37107)
                     /locus_tag="JQH65_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HAY6137954.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLAMMTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYQVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVLQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGAFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(37267..38157)
                     /gene="hcaR"
                     /locus_tag="JQH65_000437"
     CDS             complement(37267..38157)
                     /gene="hcaR"
                     /locus_tag="JQH65_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HAY6137955.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKTVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            38293..39652
                     /locus_tag="JQH65_000438"
                     /pseudo
     CDS             38293..39652
                     /locus_tag="JQH65_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
     gene            39649..40167
                     /locus_tag="JQH65_000439"
     CDS             39649..40167
                     /locus_tag="JQH65_000439"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HAY6137956.1"
                     /translation="MSAQVSLELHLRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYQAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            40167..40487
                     /locus_tag="JQH65_000440"
     CDS             40167..40487
                     /locus_tag="JQH65_000440"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HAY6137957.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGSDIFIDLP
                     EAQP"
     gene            40484..41296
                     /locus_tag="JQH65_000441"
     CDS             40484..41296
                     /locus_tag="JQH65_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HAY6137958.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            41306..42322
                     /locus_tag="JQH65_000442"
                     /pseudo
     CDS             41306..42322
                     /locus_tag="JQH65_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
BASE COUNT        10032 a        11713 c        10642 g        10008 t
ORIGIN      
        1 acgacaggtt tagccgttgt tattaccaca acgtataacg aaacgcgtat tcagccaaat
       61 actaaatgtc ctgaaaagag ctaataatca ggaggagtca tgaagaaagt ttttctttgc
      121 gccatcttgg cctccttaag ctatccggct atcgcctcat cattgcagga tcaactctct
      181 gctgtagcag aagcggaaca gcaaggtaaa aatgaagagc aaaggcagca tgacgaatgg
      241 gtcgcggagc gcaacaggga aatccagcaa gagaagcaac gtcgcgcaaa tgcccaggtc
      301 gccgctaaca aaagagcggc aacggcagca gcaaataaga aagctcgtca ggataaactg
      361 gacgccgaag cctctgcgga caaaaaacgc gatcaaagtt atgaagatga gctacgcagc
      421 ttagagattc agaaacaaaa actggcgctg gcaaaagaag aagcccgcgt taagcgagaa
      481 aacgaattta tcgatcagga actgaagcac aaagctgcgc aaaccgatgt ggtgcaatct
      541 gaagctgacg ccaacagaaa tatgactgaa ggcggtcgca atctgatgaa aagcgtgggc
      601 aaagcagaag agaacaaatc ggacagctgg tttaattaat cgatgttagt aacttcaagc
      661 ctatgattct tgaagataaa aaaccctctg tagtaacaga gggttttgtt cattcatagt
      721 gcagggatta aaatcattcc cactcaatgg tagctggcgg cttgccgctg atgtcataca
      781 ccacgcggga aataccgttc acttcattga taatgcggtt ggaaacgcga ccgaggaaat
      841 cgtacggcag atgcgcccag tgtgcggtca taaagtcgat ggtttcgaca gcacgcagag
      901 agacaaccca gtcatactta cgaccatcgc ccattacgcc aacggaacgt accggcagga
      961 acacagtgaa cgcctggctg actttgttgt acaggtccgc tttacgcagt tcttcaatga
     1021 agatggcgtc agcacggcgc agcaggtcac agtactcttt cttcacttca cccagcacac
     1081 gaacgccaag gcctggtccc gggaacgggt gacggtacag catgtcgtac ggcaggccca
     1141 gctccagacc aatcttacgc acttcgtctt tgaacagctc tttcagcggt tcaaccaggc
     1201 ccatcttcat ctctttcggc aggccgccca cgttgtggtg agatttgatg acgtgtgctt
     1261 taccggttgc agaagccgca gattcgataa cgtcagggta gatggtgccc tgcgccagcc
     1321 acttcacgtc ttccagtttc agcgcttctt catcgaacac ttcaacgaaa acgcgcccga
     1381 tgattttacg ttttgcttcc ggatcgtttt cgccagccag cgctgacagg aagcgatctt
     1441 ctgccggtac gtgaacaatg ttcagaccaa agtgatcgcc aaacatatcc agaacctgct
     1501 ctgcttcgtt gagacgcagc aggccgttgt cgacgaatac gcaagtcaga tttttaccga
     1561 tagcgcggtg cagcagcatt gcggttacgg aggaatccac accaccagag aggccgagga
     1621 tgactttatc gtcgcctacc tgctcgcgga tgcgagctac agcatcgtcg ataattttcg
     1681 ctggcgtcca cagggcttca cactggcaga tatcacgcac aaaacgctcc agcatgtgca
     1741 taccctggcg ggtgtgagtc acttccgggt ggaactgtac gccatagaag cgtttttctt
     1801 cgttagccat aatggcaaac gggcagcttt cggtgctggc tacggtgacg aagtcggacg
     1861 ggatagcggt aactttatcg ccgtggctca tccagacatc gagcagcggt ttaccgtctg
     1921 cggtcagcgc atcttcgata ccgcgaacca gtgcgctgtc gtttacgact tcaacctgcg
     1981 cgtagccaaa ttcacgttcg ttagaggctt caacgtgacc gcccaactgc attgccatgg
     2041 tctgcatgcc atagcaaacg ccgaataccg gtacgcctgc ttcaaagaca tactgcggcg
     2101 cacgcggact gttttcttca gtagtacttt ccgggccgcc ggaaagaata atgccgcttg
     2161 gattgaagtc acgaatttgt gcttctgtca catcccacgc ccacagttcg cagtaaacac
     2221 ccagctcacg cacgcggcgc gcaaccagtt gagtgtactg agaaccgaag tccagaatga
     2281 ggatgcgatg cttatgaatg ttttccgtca ttgacgctta ttccgaggca agtgaaacag
     2341 ataatataaa tcgcccgaca tgaagtcggg cgaagagaat caggagccca gacggtagtt
     2401 cggggactct ttagtaatgg tcacgtcgtg aacgtggctt tcctgaatgc ccgcaccgct
     2461 gatacgtaca aactccgctt tagtacgcag ttcgtcgata gtaccacagc cggtcaggcc
     2521 catacaggag cgcaggccgc ccatctgctg gtgaatgatc tctttcaggc gacctttata
     2581 ggctacgcga ccttcgatac cttccggcac tagtttgtcg gcagcgttgt cactctggaa
     2641 gtaacggtca gaagaacctt tggacatcgc acccagagag cccataccac ggtaagattt
     2701 gtaagaacgg ccctggtaga gttcaatttc acccggagat tcttcagtac ccgccagcat
     2761 ggaacccacc attaccgcgc ttgcgccagc ggcgatagct ttggcgatgt cgccggagaa
     2821 gcgaatacca ccatctgcga taaccggaat acctgtgcct tccagcgctt caactgcgtc
     2881 agcaacggca gtgatctgcg gaacacctac gccagtaacg atacgagtcg tacagataga
     2941 accagggccg ataccgactt taaccgcgct gcaaccagct tctgccaaag cgcgtgcacc
     3001 tgcagctgtt gccacgttgc cgccgatgat ttgcagatcc ggatatttag cacgggtttc
     3061 acggatacgc tgcaaaacgc cttctgagtg gccgtgggag gagtcgatca gcagaacgtc
     3121 aacgccagcg gcaaccagcg cgtcaacacg ctcttcgtta cccgcacctg cgccaactgc
     3181 agcaccaaca cgcagacggc cttgctcgtc tttacaggcg ttcggtttac gttccgcttt
     3241 ctggaagtct ttcacggtga tcatgccgat caggtggaat tcgtcatcaa ccaccagcgc
     3301 tttttcaacg cgtttttcgt gcatttttgc cagcaccact tcacgggctt caccttcacg
     3361 cacggtgacc agacgctctt tcggcgtcat gtaaacgcta accggctggt tcaggtcggt
     3421 aacaaaacgc acgtcacgac cggtgataat acccaccagt tcgttttctt cggtaacaac
     3481 cggatagccc gcaaaaccgt tacgctcggt cagttctttc acttcgcgca gcgtcgtggt
     3541 tggcagaaca gtctgcggat cagtcaccac accagattcg tgttttttca cacggcgaac
     3601 ttcttctgcc tggcgttcaa tggacatgtt tttgtggata aagccgatac cgccttcctg
     3661 agccagagca atagccaggc gcgcttccgt tacggtatcc attgctgcgg aaagcatagg
     3721 gatattcaga cgaatagttt tcgtcagctg ggtgctgagg tcagcagtat tcggcagaac
     3781 ggtagaatga gcaggaacga ggagaacgtc gtcaaacgtc agagcttctt tagcgatacg
     3841 tagcatgggc aatatctcga ccagagtggt taataaatat tgccgcggca ttatacagag
     3901 cgtaaccgat tgcatctacc cctttttgca aaaaatgctt gctatccccg aagggcgggt
     3961 tactatctac tgaataacct gctgatttag aatttgatct cgctcacatg ttaccttctc
     4021 aatcccctgc aatttttacc gttagtcgcc tgaatcaaac ggttcgtctg ctgcttgagc
     4081 atgagatggg acaggtttgg atcagcggcg aaatctctaa tttcacacaa ccggcttccg
     4141 gtcactggta ctttacactc aaagacgaca ccgcccaggt acgctgcgcg atgttccgca
     4201 acagcaaccg ccgggtgacc ttccgtccac agcatggaca acaagtttta gttcgcgcca
     4261 atattacgct ctacgagccg cgcggtgact accagataat cgttgagagt atgcagccgg
     4321 ccggtgaagg gctgctgcaa cagaagtacg aacagctcaa agcgaagttg caggctgaag
     4381 gtttgttcga tcagcaatac aaaaaaccac ttccctcccc tgcgcattgc gttggtgtga
     4441 tcacctcaaa aaccggtgct gcgctacatg atattttgca tgtgttaaaa cgtcgcgatc
     4501 cttctctacc ggtgatcatc taccccaccg ccgttcaggg cgatgacgca ccggggcaaa
     4561 ttgttcgcgc cattgaactg gcgaatcagc gcaatgagtg cgacgtgttg atcgttgggc
     4621 gcggcggcgg ttcgctggaa gatttatgga gttttaacga cgaacgcgta gcgcgggcga
     4681 tttttgccag ccgcattccg gtcgtcagcg ccgtcgggca tgagacggat gtgaccattg
     4741 ccgattttgt tgccgatctg cgtgcgccaa cgccgtctgc cgccgctgaa gtagtgagcc
     4801 gtaatcagca agagttactg cgccaggtgc aatcgacccg tcaacggctg gagatggcga
     4861 tggattatta tctcgccaac cgcacgcgtc gctttacgca gatccatcac cgattacagc
     4921 aacagcatcc gcagctccgg ctggcacgcc agcaaaccat gcttgagcgc ctgcaaaagc
     4981 ggatgagctt tgcgctggaa aatcaactta agcgtaccgg gcaacagcag cagcggttaa
     5041 cacagcggct gaatcagcaa aatccacagc cgaagattca tcgcgcgcaa acgcgcattc
     5101 agcaactgga atatcgttta gcagaaaccc tgcgcgtaca gcttagcgcc acgcgtgaac
     5161 gtttcggtaa tgcagtaacg cacctcgaag ccgtaagccc actgtcaacg ctggcgcgtg
     5221 gatacagcgt tactactgct actgacggca atgtactgaa aaaagtgaag caagttaaag
     5281 cgggtgaaat gctaaccaca cgtctggaag acggctggat agaaagtgaa gttaaaaaca
     5341 tccagccagt aaaaaaatcg cgtaaaaagg tgcattaagc cagcacgaat tcaacgcgtt
     5401 ttttcgatat caagccatgc ccgtgctgac agaagtaatc tactgcaccg caggctttta
     5461 acacctgcaa cggttgatgg cagtctggac aaagagcttt catttcaata atttccccgc
     5521 aggatgggca acgcgcatgg ccgttatcct gatcaagcac atgctgacat tgtggacaat
     5581 gcagttccat atgaattcct ccggtagcca tccctctaca ttcatagagg gaatggcaga
     5641 taaaatactt acggataatt atttattttt cttgatgtgc ttcatcagac gcttacgttt
     5701 acgcatctgg gttggcgtca gggtgttacg cttgttcgca tacgggtttt ccccttcttt
     5761 gaactgaata cgaatcggcg atcccattac gtccagcgat ttgcggaagt agttcatcaa
     5821 gtagcgcttg taggaatcag gcaggtcttt cacctgatta ccgtgaatca ccacaatcgg
     5881 cgggttataa ccaccagcgt gggcatattt cagcttcaca cgacgaccgc gtaccagcgg
     5941 cggttggtga tcttcaacag ccatcgtcat gatgcgcgtc agcatagagg tccccacacg
     6001 acgggtggag ctgtcatacg cttcacgtac tgattcaaac aagttaccaa caccactgcc
     6061 gtgcaaggca gagataaagt gcacacgagc aaaatcgata aagcccagac ggaagtccag
     6121 cgtttctttc acctgctctt tcacttcctg gctcaggcca tcccacttat tcaccacaat
     6181 gacaagtgag cgcccactat tgagaataaa gcccagcagc gagagatcct gatcggaaat
     6241 accttcgcgc gcatcaatca ctaacatcac cacgttggca tcttcaatgg cctgcaacgt
     6301 tttgattacg gagaatttct ctacagcatc ggtgattttg ccgcgtttac gtacgccagc
     6361 ggtgtcaatg agcacatact cacgtccatc gcgttccatt ggaatgtaga tactgtcacg
     6421 cgtcgtgcca ggcatgtcat aaacaacaac gcgctcttca ccaagaatac ggttagtgag
     6481 tgtagactta cctacgttcg gacgacccac aatcgccagt ttgatcggca gactttgcgg
     6541 gtcgaagtcg tcttcctctt cttcttcgcc gttctcttcc acttcaaatt gcgcccagta
     6601 ttcagcgtct tcgtcgactt cctcttgcgg tgcgagatct tccatccacg gcagcagcac
     6661 atgctccagc agacttaata cgccacgacc gtgagacgcg gcgatcgggt agatttcacc
     6721 taagccaagc gagtagaaat caaccactgc ctgatcggga tccagaccgt cagttttgtt
     6781 tgccaccagg aaggtcggtt tttcacggga gcgcagatgt ttggcaatcg cttcatctgc
     6841 cggcatcagg cccgcgcgcg catccaccat aaacagtacg acgtccgctt cttcaatcgc
     6901 cagcagcgac tgttccgcca tgcgggtttc tacaccgtct tctgtgccat caatcccgcc
     6961 ggtatcaata cagataaact cacggccttc aatttccgca cgaccgtact tacggtcacg
     7021 agtcagaccc gggaaatccg caaccagcgc atctcgggtg cgagttagac ggttaaataa
     7081 cgtggatttt cctacgttag ggcgcccgac aagcgcgacc acaggtacca tgtttaaagc
     7141 ctcattttta taaatcatca gacaacgcac gctatattcg cgtcgttgtt aaaaacagga
     7201 aaacggcccc tgtccaggag ccgttttcaa agtgaacgac agagacgatt aacgtgtaat
     7261 agagtacacg gttccgtctt ttgcctggat cagcagtttg ccgtcagcgg caaccggttc
     7321 agtctggaaa ccggaactat caactttttg ctgggcaacg aaacgaccat cttcgacatt
     7381 aatccagtgc agataacctt cactgtcacc gaccaccagg ttgccatcat acagcaccgg
     7441 agaagtcagc aggcgatgca acagatcgct ttgtgtccac agcgtaacgc cgccatcaat
     7501 ggtcaacgcc atcacccggt cattttgatc gaccagatag atgcgattgc cgtcgacgat
     7561 gaaatcattc accgaaccca gttcgcgttt ccacataatc tgaccactgc gcagatcaag
     7621 cgccgtcagg ttaccattat aggccagcgc gaaaacaacg ccgttaacaa cgacgggagt
     7681 cgtgtcaaca tcgctcagac ggtcaatttc ggtagaaccg gtcgcctggg aaatacgctg
     7741 ctgccaaatc atctggccct gttccatcag cactgcgctg acgcgaccat tatcgccccc
     7801 cacgacggcc gcaccaaaag ccgttgccgg cgcagactcg ccacgcaaag agagcgaagg
     7861 catatcgagg ttaactgtcc atttgacagc gccgtcagct tcgttcagcg cttgtaactg
     7921 accgttactg gtgtggatta acaccagacc gtcgctgacc accgggcgcg aaagtgcttc
     7981 acccgcgact ttagtttgcc atgccacagt accatcgctg gtattcagcg cgtaaacctg
     8041 cgccttttcg ctgccaatgt agacatgccc accagacacg gtcacaccgc cagaaagtaa
     8101 tgcaggctct ttagagaacc agccatcttt ctcggccagg ctgacagacc agatttcttt
     8161 gccatcatcc gcattcagcg cttttaccaa accagcgcgg tccgctgcat agacaacgtt
     8221 gtccgccagt gccggatgaa gattggaata gaagttgcca atgccgctac caacggaagt
     8281 actccacgcc gtggtcggcg taaactggtt ttcaacggtt ggcaatgggg acatctttac
     8341 cacatcttct tcgctgttaa acagcgaaca gccgcttaaa agggtaacgg aaagcagtcc
     8401 tggcagcagt aatttacgca attgcatcgg gtccctctca gatggacaaa ttattaattt
     8461 tcatctgcat catttcgctc agtgccggag taacatcgct tttcacgcct gcttcccatg
     8521 cactacgcgc accttgctta tcacctttgc tcagcaatgc ttcaccacgc aggtcggcaa
     8581 caatggcagc ccacccttca cctttgatgg tatcaagggt tttcagcgcg gcatcagcct
     8641 gcttgagctg tacctgaacg cgagcaagac gcagatttat cacggctttg agattttcat
     8701 cgctcgtgtc tgccagcccc tgttgtaact gggcggcagc tttctctagt tcatttttgt
     8761 caacaaattg ctgcgcaagt tccaaagaag ccagcgcacc ataagtattt ttattttcag
     8821 cagcaaattt ttccgccgcc gggatgctat ccggtttgcc ttcgctcact gcggtaaccg
     8881 cattttgata ggcaagagaa gcggagcgtg cagaatcaac ctgatggctg ttccagtagc
     8941 gccagccaat cagtgcgcca acgcccaaaa tcaccccaac agccagtgct ttgccatttt
     9001 cagcaaaaaa gcgtttaacc gcttctacct ggtcgttttc gttctcgtaa atttccacgc
     9061 tgtccttctc cttccttaac ccagtaacgt gcgcaaatgc gcggctacgc tatcctgcgc
     9121 aactgccgtt tgctcaccag agcgcaaatc cttcactact gctgtgccgt tagtcacttc
     9181 agactcaccc agcaccacag caacgcgggc accccattta tcagcacggg caaactgttt
     9241 cttaaagttg ccgccgccgt ggttggtcat caatttcacg ccctgtaatt catcacgcag
     9301 acgctcagct aatgccatag ccgcagattg tgtatcagca cctgaagcca ccaggtatat
     9361 atcgacaata ggatcggctt taaattccgg attaacggcc tgtactaaca atacaagacg
     9421 ttcgaggccc atcgcaaaac cgacagccgg tgttgcacga ccgcccagtt gttccacaag
     9481 accgtcataa cgaccgcctg cacacacggt gccctgggag ccgagactgt tagtcaccca
     9541 ctcgaaaacg gtacggttat agtaatccag accacgcacc agacgctggt ttacggtgta
     9601 agcgatcccc gcgctctcaa gcagtttgca cagaccggca aaatgctcac gagattcctc
     9661 gtccagataa tcacctaatg ccggagcgtc gttgagaagc gcctgcactt ccggattttt
     9721 tgaatccagc acgcgcagcg ggttagtgta catgcggcgt ttgcagtctt cgtccagctt
     9781 ttctttatgc tgctcaagga atgccaccag cgcatcgcgg taattggcgc gtgcttccag
     9841 cgaaccgata gaattcagct caagagttac gtgttcggaa atacccagcg cgcgccacca
     9901 gcgggcggtg agcataatca gttcagcgtc gatatccgga ccttgcagac cgaaaacttc
     9961 gcagcccaac tgatggaact gacgataacg ccctttctgc ggacgctcgt gacggaacat
    10021 cggcccgata taccacagac gctgttcctg attgtacaga agaccatgct cgatgccggc
    10081 gcgtacacag cccgccgtcc cttcagggcg cagagtcagg ctgtcgccat tgagatcctc
    10141 aaaggtgtac atctcttttt caaccacgtc ggtgacttca ccaatcgcac gtttgaatag
    10201 cggggtctgc tctacaatcg gcaagcggat ttcactgtaa ccgtagctgc cgagcacgtt
    10261 tttcagtgtg ccttcaatgc gctgccagat ggccgtttcg ccaggcaggt aatcgttcat
    10321 gccgcgaatg gcttgaatgt tttttgccac gtttattctc tttctgaata taaaaatgaa
    10381 ccctcaacgc ttccctcaat gtttggggag ccatgcgggt tcaatcatac acgggaagcg
    10441 aggcgcttcc catcacgtta ttatttttca acctgctgaa cgtcaattcg acgcgcttca
    10501 tccagctgac tggctttcgc acgaatgcgt gcttccagct ggtcgatcat atcgttgttg
    10561 tccagacggt ctttgcgcac gccatcttca tagaggccgc ttttcttgtt gccgccggtg
    10621 acgccgagtg tagaaaccag cgcctcacct gggccattca ccacgcagcc gataatagaa
    10681 acgtccatcg gagtgatgat atcttccagg cgttgctcca gcgcgttaac cgtaccgata
    10741 acatcaaatt cctgacgcga acaggtcggg caggcgatga agttgatccc tcgcgaacgg
    10801 atacgcagcg atttcaaaat atcgaaaccg actttgatct cttcgaccgg atcggccgcc
    10861 agcgatacgc gcagcgtgtc gccgatgcct tcagacagca gcagacctaa accaatggcg
    10921 gattttactg ccccgctgcg tgcaccaccg gcttcggtga tccccagatg cagcggctga
    10981 tcgatctgtt ttgccagcaa acgataagac tcaacagcga ggaagacgtc agacgctttc
    11041 acgctgactt tgaactgatc gaagttcagg cgatcgagat gatcaacatg acgcatggcg
    11101 gattccagca acgcctgcgg cgtcggttcg ccatactttt cttgcagatc tttttccagc
    11161 gatccggcgt taacgccaat acggatcgga atgtttttat cgcgcgcaca gtcaaccacc
    11221 atgcgaatac gctcttcatt accgatattg ccagggttaa tacgcagaca atcgacgccg
    11281 tattccgcta ctttcagcgc aatgcgatag tcgaagtgga tgtcagccac cagcggcacg
    11341 ttaacccgct gtttgatgag tttgaacgct tctgccgcgt ccatcgtcgg tacggagaca
    11401 cggacgatat cagcgccaac gcgttccagc gccttgattt gattgaccgt tgcttcgaca
    11461 tctgtcgtac gcgtattggt catggactgt acggcgatgg gagcaccatc gccaatcggc
    11521 acattcccaa cgtaaatacg tgttgatttt ctacgttgaa ttggagcctg gttatgcatg
    11581 aaaaatctcc cgcgttaccc gtctgttact gcgccggtga ttgttcggca ttgagggtca
    11641 gacgcgcaac ctggttagtt ctgataaaac gactcagatc gacaggtttc ccttgatact
    11701 ggatctgtac tgcggctggc gcaccaattt tcagtttgta cggtgcctgg ccggttaagt
    11761 tcaaattacc gtctttacgc tgcataccgc taaacaattt tttaccggta gcatcagtga
    11821 cctccagcca gcaatcggca gtaaagttca tcaccagcgc attcggatca gccaccggcg
    11881 tggtcacgcc agcctgatcg gttggcaacg gcgcagcacc atctggcgtt gttgctgccg
    11941 ttggtgccgg ggtcgcggcg gtatcaacat ttgcctgcga aggcgaaaca accgcattct
    12001 gttgcggatc aacagctggt gctggcgcag ttacggcagg tgtttgcgtg ttggttgcgg
    12061 tagtatccac agacgctggc ggcgtggacg ttgtagccgg gtctgtagtt gtcgacgtat
    12121 ttaacggaac actctgcccc tgctcgctat tactgctcag ttccgccgaa gattgatcgg
    12181 ccatagtggt gatctcttcc tgctgagctt tgtggtcttg ccaccaccag gcaccgctca
    12241 ggccgataac cacaaacaac accagccagg tgaaggtcat cagccagccg tcacgttttt
    12301 tgcggcgttt accgagggaa aaactctgca tcggcgcaac tttcgcagcc cgtagtggag
    12361 cctgcttttc cagccctagc agcagttctt cttctggaat atgtaccaga cgcgcataag
    12421 agcggatata tccgcgcagg aatgttgaag caagatcggc gggtgcctta tcttcttcaa
    12481 tgtcgcgtac cgtggaaacc ttcaggcaaa gtcgctcggc aacggcctgc tgactaagtc
    12541 ctagttgttc gcgagcatta cgcaggcgag cgccggtagt aagtgcttca ttttggtcgt
    12601 gcgtggcttc agtattcatt cgctgcaggt acgtttaaat gagaattgag atgccggtga
    12661 accatcatgc tcacccacac cgcgaaacat ccgttaagtt aaccgttatc atacagtata
    12721 agaccgtctg tgtgctcgtg acaaagccca cattcactaa ggctaactta ctgttgcatc
    12781 gttacatact gccttaaagt cagcaaaaac gcaccgttaa tattgaccag acaattgcaa
    12841 cttaatactt cattcataaa agattacgcc acggtacata aagtaaccgt ggcgtaatgg
    12901 ctatcagacc gctttaatgt cgatggcttc accctgcatc cgtttacgca gggtacgttt
    12961 cgtacggtcg ataacatcgc ccgccaactg accacaggca gcatcgatat catcaccacg
    13021 agttttacgc acaatagtgg tgaaaccgta gctcatcagc acttttgaga aacggtcgat
    13081 acggctgttc gaactgcgtc catacggcgc gcccgggaac gggttccacg ggatcaggtt
    13141 gatcttacac ggcgtatctt tcagcaactc cgccagttgg tgcgcgtgtt cagtgccgtc
    13201 gttaacgtgg tcaagcatca cgtattcaat agtgactcgg ccctgattgg cgttggattt
    13261 ctccagataa cggcgcaccg cagcaaggaa cgtttcgata ttgtactttt tgttgatcgg
    13321 cacaatttcg tcacgaattt cgtcgttcgg cgcgtgcagg gaaattgcca gtgcaacgtc
    13381 gatcatatcg cccagtttat ccagcgccgg aactacaccg gaagtagaaa gcgtgacgcg
    13441 acgtttagac aggccaaaac cgaaatcatc cagcatgatt tccatcgccg gaacgacgtt
    13501 gttcaggttg agcagcggct cgcccatgcc catcatcact acgttagtga ttgggcgctg
    13561 accggtgact tttgctgcgc cgacgatttt cgccgcacgc cacacctggc cgataatttc
    13621 cgacacccgc aggttgcgat taaagccctg ctgggcggtg gaacagaatt tacactccag
    13681 cgcacacccc acctgcgaag agacgcagag cgtggcacgg tcgtcttccg ggatatacac
    13741 cgtttcgacg cgctgatcgc caacggcgat cgcccattta atggtgccgt cggatgaacg
    13801 ctgttcttca accacttccg gtgcgcggat ttccgccacc tctttcagtt tgccgcgcaa
    13861 cactttgttg atgtcggtca tctcatcaaa gttgtcgcag caatagtgat acatccactt
    13921 catcacctga tcggcgcgga agggtttttc acctaaatct ttaaaaaact cccgcatctg
    13981 ctgacggttg agatccagca ggttgatttt tccatctttc gtggtgacgt tttcaggtgt
    14041 gactaattgt tcagacatat gctattccgg cctcgttatt acacgttatg gcccctggag
    14101 ggttgaaaaa agaaacgccc cggtgagcgg ctgctcgtcc gggggcgctg cattgtacaa
    14161 attctggcgc acggatgcca cgtttgcacg cggcatttac gaaattatta acgggtgcgc
    14221 gggcacactt cgccttcgcc aaagaaataa gcgatttcgc gagcggcaga ttcgacggaa
    14281 tcagaaccgt gggtaccgtt ttcggtcagg ctgtcagcgt aatcagcgcg cagagtacca
    14341 gccagtgcgt ttgccggatt ggtcgcgccc agcagatcgc ggtgacgctg aacggcgttt
    14401 tcaccttcca gcacggaaac cacgatcggg ccagaggtca tgaattcaac cagaccatca
    14461 aagaacggtt ttccatcgtg ttcagcataa aagccacgtg cctgttcaac ggtcaggtgc
    14521 agcattttgg tgccgacaat tttgaaccct gcagcttcaa agcgcgcaaa gatattacca
    14581 atgacgtttt ttgctaccgc gttcggtttg atgatggaaa aagtacgttc aatagccatt
    14641 tttacctctg taaattgttc tgttgttgtc tgtaccagcg tacgagatgg cgcggattat
    14701 aatgagcaac agggccgttg actattgatg aaggtaacat ttttttaaaa taaaatgagt
    14761 tttagcaaca atcagggaca aaacagacta ttgcatgaca aatttcactg tcgcgatttg
    14821 ccccacgtca tccatcacca gcaattgata atcaccttta tccgtcaaat gcagggtcac
    14881 gttgcgcccg cgttcagtta acggttcgcc attcagaaac caccagcgtt cacctgcccc
    14941 tccacttgat tgcaacggca aagtcgcttc cgctgcgccc ggtaaacgtt taataatcgc
    15001 gccatcgcga acgcctgtca gctgcagcgg gagctgggca tagtgaccgt atggcgggca
    15061 acttgtcgag gccggtggta agcgcacagc gcgacgttct gatgcgggca gccagggttc
    15121 cagcggtagc ggccagacat ttatcatttc ttgtcgcgct tgcgggcaat cagcggcaac
    15181 acgtttgcca ttttcatcca gccagatggg gaaacgaatg ccattaatgc cttcctgctc
    15241 cggcagtaat agagttggcg gctgactccc gtccagcagc caggttgcca ggcggcggcg
    15301 acagttaccg tcaccttccg gcaaagactg tccgcccggc cagcagataa cgccacgagt
    15361 gactgagttc gggcgcgggt cttccggcag attcgcactg cgcgacagta agatattatt
    15421 gacctgattc aacaatggta cggcactggc aaagccaaac tgaccaacaa cgggcgtgca
    15481 gtccggtctg ccagtccaga tcccaatgac atagcgagcg ttaaccccaa tcgcccaggc
    15541 gtcacgatag ccatagctgg tgcccgtttt ccatgccagt ggggcgacgc gcggcaaggc
    15601 actatccggc aagggttgcg cttcatcagc cataatccgg cgaatgatcc acgccgcccc
    15661 cgacgacatt aaaggccatt caagcagcgg atcgtcaggc tgtaagcgca atttgcctgc
    15721 cttgccgtgg cgagcaaacg cggtatacgc tgccgccata tcttccagtt ttgcaccagc
    15781 gccgccgaga atgagtgaaa gattcggcgc agcaccgttg ggcaaatata acggcaatcc
    15841 aacattgcgt aactttgccg caaaccgttt cggtccatag gcttccagca cctgcacagc
    15901 aggtaagttc agcgagcgca ccagcgcctc gctcatgctg atcgggccat gaaaaccgct
    15961 atcaaagtta cctggtcgat aatcaccggt gcgccggggg acgtcttgca gcagtgatgc
    16021 cgggtggatc aagccttcat ccagcgccag accataaaca aacggtttga gcaccgatcc
    16081 tggcgatcgg atcgcattga ccatatcaac atgaccaaag cgtgaatcat cgttgagatc
    16141 aaccgatccc acccagccgc gaacacgcat atcggtatga tcaaccacga tcatcgccag
    16201 tgagctgcgc ggtggcaacc gccctttcca gttttgcgcc agttcttcca gacgtcgttg
    16261 aagaccggca tccagcgtag taacgatttt gtcgcttttg cttttaccga gcatcatgcg
    16321 cgaaaacagc ggtgccagtt gcggcatttg tcggggtgcc agccagatgg gttcttccct
    16381 tgactctttt acctgctcac gggaccacac accttgcacg gccatccgtt cgagcacttt
    16441 attacgcgcg gcttcggcac gctccggcca acgatccggg cgcagtcggc tgggcgcttg
    16501 cggtaacacc gccagcattg ccgcctcgga atagcttaaa ttcgcaggcg attttccgag
    16561 ataagcccaa cttgccgcac cgatcccctg caacgtaccg ccaaacggag cgcggttaag
    16621 atacaagatc agaatttcac gcttagacaa atgccactcc agttgcaacg cgcgccagag
    16681 ctggagaatt ttgccaccaa atgttttggg gtgaggatca agcagacgag caacctgcat
    16741 agtgagcgtg ctgccaccag aaataacccg tcccgaagtg agatcttgcc atgctgcgcg
    16801 cgccaccgag aatggattca cccccggatg cttccagaac cagcgatctt cataattgat
    16861 cagcgcttca aggtaacgtg gagacacatc ttcgattgtt accggataac gccagatgcc
    16921 atcagcatcg gcgaagcgcc agagcggcgt accatcctgc gccacgacca ctcgtgcggg
    16981 attgacttca tgcagcggta gaggccagag tttatctgct ccccacgctg caagaaaaag
    17041 gagaaatggc acagctgcca gcgttatcca gcagccgcgt ttggttaaca agcgaagcat
    17101 ttacggtcgg acaatcagca gatcttcagc cgcgccggtc gcccgccatt ggggaacata
    17161 cattgattcc accatcggtt gcggtacctg atacgttccc ggcgtcaccg cccgcgccag
    17221 atacaccagc gttaccggtt ggtattcatc aacggcaacc gccgccacaa agcgatcgtc
    17281 acggaactca atgtgcttaa tgctcgcctg ctgcatctgg ttcagtaagt tttgcacttc
    17341 gtcaccactt tgctccaggc tggcgctacc gttcgccaga ttctgatttt ccagttccag
    17401 acccgcaggc agcagatcca cgactaacgc atccggcacg ctgttactgg cttttacctg
    17461 caaccagacc agcaccagat cgccgctacg taacgagtcc agcgatttgc tcttaccatc
    17521 agtaccaaga atatgacgct cgatttgcag cacattgttc gccggtaaag gtgcggattg
    17581 cggataaccg ctggcatcca tacgcaacca taacggctga tcgccactgt ttctcacctg
    17641 caaggtggca agttgatcgc tattcagatt gctgtttagc gttttctcgc ctgtcagcgg
    17701 ctcagctgag aaagaggttt gcgcctgcca tttacccggt aaatcctgaa tcgtacgggc
    17761 agccaggaac aacgcgttac tttcctgcgt cgatagccag cgttcaccaa acgcctgctg
    17821 cgaaagtgtg ttcagcaaag tgtactgctc atcgggtagc agtttatttt cttccagcaa
    17881 ggagagcatt aacgcgttgt cgcgcagtgg actaccgtaa tcacccagcc atatccgctc
    17941 atcactattt ctcggcgttt tcagcgccag cacaatcgct tcttcaccac gcgttgcatc
    18001 acccatggtt ttcagcgcaa tgccaagttg cagcagcggt aaaccagaag cggcatctgc
    18061 acgatgctcc cagatttcac gcagcgcacc cagtggtgct ttttgctgac gggccagcac
    18121 cagcgcagcg taagactgaa cggcgaattt actggctttg agattatccg cgtacgggat
    18181 cgacatcatg cccggatctt gtaaatagcg cagcagacgc tcattacccc ggttaatggc
    18241 gtctgtcggc acgctgtaac cctgttcgcc tgcgcggacc aggaaatcca tcacgtaagc
    18301 cgtcagccag tactcttcgt caccgttttt atcccacagc gcaaagccgc cgttatcacg
    18361 ttgcatttgc agcaaacggg aaatgccgat atcgaccgat gcacggcgtt tctcatcact
    18421 gtcgcctttg atgcccaacg cctgcagttg ggctgcgttg gtataaagtg acgggaacag
    18481 gccgctggcg gtttgctcaa gacagccgta cggatacgct tttaactctt tgatataacg
    18541 tgcaatgttc agcggtggtt tgccgctcaa caacaattgc ccttccagcg taacaggcga
    18601 gaagttttgc aatccatccg ccggaatcgc ccatgtctca ccaggctgta acgccgtacc
    18661 gtaattaacc gtttgtgccg ggaacgccgg acggacgccg attttccact gcttatgctg
    18721 atcggcaacg gtttcacccg gtaacgctaa cccgctaatg gtggcctgaa tttctccatc
    18781 gccataaccc ggcaatgctc gcaccgggat aaacagcgta gtacgcacac ctggcgctaa
    18841 ttcaacggct gcgggtgaat cgctgaccag ttcaagcaaa ccactggcgg tcagggcaac
    18901 gttcagtttt tgcggtttat cggtaagatt agtgatatcc agcgtcagac gcgaggtatc
    18961 gccactcgcc ataaagcgcg gcatgttcag ttcagcaatc accggtgcgg caactatcac
    19021 tttgctttcg ttgctaccga aatcatctgc cgtccaggct tgcgccatga cgcgcaattc
    19081 accgttaaaa tcgccaatcg gcagtgtaac cgagccttcg ccctgttcgt tgagcgttac
    19141 cggcagcgcc tgctgcgcga caatattgac gtgattgacc agcggtttac caccacgttt
    19201 cagctcatca ccatcgccac cgaaacgcag agctgccaga cgcccctgac cttcaataac
    19261 ctgaccgtaa atatcgtaaa tgtctgcgcc atagcgtttc tgaccaaaga acgcctgcca
    19321 cggatctggc gtgacgtagt cagtaatatt cagcacacca ctatcaacgg cagacaccag
    19381 cacattcacc tgtttaggct tctcgccatt tttagtgctg gctttaattt tcacggttaa
    19441 tggttgattg ggacgcattt ttgctggtgt ttccagcgcc agatcgaggc gacggttttc
    19501 atcgccaagc ggcagatgca acacaccaac cgcgcgtttt ggcgtcgcgg agcgagattt
    19561 atcgctagga cgtaccacca gcgtacttaa atacagatca tgacgattcc aggttttatc
    19621 gaccggaatc gtcagatcca gcccttgagc cggaacatca atctcttgcc accacagcgg
    19681 cccttcactg gactcgacca tcgcataacc tttacccgcc gttggcgcgg cgatatgcaa
    19741 cttaatggtg tcgccagggc gataactggc tttatccagt ttcagcgtga cacggtcggg
    19801 tcgcactgcg ccgctaccgt cgctgttgtc ctgccagcta tagccagccc agaaacgaac
    19861 actactgacc gcttcattcg gcgctttgac ttccagacga taagcgcccc actctaccgg
    19921 aaaactgacc ttgccggttt cgtccgcttt cagatccaga gtttgttcat tttcgatcag
    19981 atctttttga tcaaactgtg actgccagcc ttcatcttct gaccagttcc agtagtaatc
    20041 gcggcgttcg cgaatcaggc gcacctgcaa gcccgacacg gcttttttca cgccttgtgc
    20101 atcgctataa acgatgtcaa aagcggcgtt actgccttca tcaacaatgg gctgtttcac
    20161 cgtgctgtca gtgcgataat cgtaaaccga tttcaaggcg aactgcggac ggagccccgg
    20221 caatgcattg gcaggccaga tagcctgctc agcgcggcgc gtcaccgggc gaccgcccga
    20281 ttccagcaaa ctgccctgga aaataacctg caatggggaa tgcgtttcct tccactggct
    20341 ttctgtagaa acttcgccgc gccctttatc atccagcgtc aactgaactt catccagcgt
    20401 gcgggaaagg ttttcggcag ctatatcgcc gaattcaaaa ccaggtaagg ctgacacagc
    20461 ctcacgcagt gggcgcagga aaagttgccc ttgcaaagta ttaccattag caggcgcacc
    20521 gtacaggtag tatcccacca cggagaattt cacttcatca ttcggcgtaa gcggggtttt
    20581 ctcaccggtc agattcagcg ccatacgctc tggcataaaa tcttcgacgt ggaaatccca
    20641 catccggtac tggttatcgc ccgtgttggc gcgaatatgc cacataccgg ttgccgcatt
    20701 gctatcgagt ggccaggtaa agtggtagag gccattctcc ggctgactaa cgacgctcct
    20761 gagtacctgc ccgtcaggtt taatcacgtc taacttgatg ggttgatcgg gcaatgcttt
    20821 accgtctgca tcgcgcagta aaccattgag gattaccgtt tcacctgggc gataaagatc
    20881 gcgtgggcca aacatgaaaa actgtttgct atagcctggc gcgccagcaa tgttaaattc
    20941 tgctaagtcc agcgccggaa gttttaaatc gagtagcgtt gtctgaccgt ctttacgtgc
    21001 cagcagtaat gccgcatttt tatcattttc cagctgcaca tgcccctgag cgtcactggt
    21061 tgcctgagtc agagtctgcc ctttctcatt taataaagag acttcaattc cttgctgggc
    21121 cgcgccgttt tccagacttt gggtaaagat atccagacga ttgtgataac ggtgagctga
    21181 aacgccgata tcacttaacg taaacagcgt cgcgggatta ctgtaatcgt aacgtccagc
    21241 ctgattcatc acagccagat acacgcccgc ctgctgaagc ggtttgatat cgcccagcgg
    21301 cagcaataat ttttcacggg tgttacgcgc aggattgaga tcaaaccgtc cggtgtagac
    21361 cagatccgcc atctgcagca gtttgtctga ctgccagttc gccagcgaat tgcggtattc
    21421 ccattggcta atgaatgctg gcagagattc tggcttaacg cggaagaagt taacatcgac
    21481 attattaacg ttgagcgcca ttaccggcag cccttcaacg actttgccag gcagcagcga
    21541 accacggctg gcaaaaccga cgctgggttg gatgtcgcgg gtagttatag ttttttcgta
    21601 atctttactg aaggttgcgt tgttgagtgc tttgacctcc ttgccaatag taacgatcaa
    21661 atcacgtttc ggttcgaggt ggcgtaaacg tagctcttta agattatctg acagctccta
    21721 ggcaccatcc actttgccgc tttttttatc gacgacatga ataacgcgtg agaaatcctg
    21781 atccgggtcg agagggatgg agaacgtcag caccagcgtg gctgcaccat caagttggac
    21841 ttcagagaga tccagcaatg tcagcacctt accggcactc tgttgggcca gtttttgtct
    21901 ctccggcacg gagagttttg ctgaactcgc gttttctgaa gaggccgctt tagtaacttc
    21961 agaaggcgca tcttttttca ccgctgttgg cgcgttatcg ttgttgtcgc accctgccag
    22021 cgccagcatt agcatgcagg cggctacgcg taactttttc atccctgcca caatggcccg
    22081 ttagcaacgt cgaataatta ttatgcgtga gaatttacgt tatgtaattc agtatcacgc
    22141 ccatacaatc cgaattttag tgagaattgt ggctcagaca gccaaatcag cctctttcgc
    22201 ttgtcgcttt tcactaaaca tccgacaatt tagcctaacc ccggcaaaaa tggagatgcc
    22261 tatgtccacg acatggtttg taggagccga ctggctcgcc gaacatattg atgacccgga
    22321 aattcagatt atcgatgccc gcatggcgtc gcctggacag gaagatcgta acgttgctca
    22381 ggagtatctg aatggacata ttcccggcgc agtgtttttt gatatcgaag cgctttctga
    22441 tcacacttcc ccgcttccgc acatgctgcc gcgcccgaaa acgttcgccg tggcgatgcg
    22501 tgaattaggc gttaacctgg ataagcacct gattgtctat gacgaaggta atcttttctc
    22561 agccccacga gcatggtgga tgctgcgcac ctttggtgta gagaaagtgt cgattctggg
    22621 gggtggactt gcaggctggc agcgcgatga tctgctgtta gaagaaggtg cagtagagct
    22681 gccggaagga gagtttaacg ccgcgtttaa tcctgaagcc gtggtgaaag taaccgatgt
    22741 attattggca agccatgaaa atacggcgca aattattgat gcccgcccgg ctgcacgttt
    22801 taacgcagaa gttgatgaac ctcgcccagg tttacgtcgc ggacatattc ccggagcact
    22861 gaatgttccg tggacggaac tggtgcgcga aggcgaacta aaaacgaccg atgaactgga
    22921 tgcgatattt tttggtcgcg gcgtcagcta cgacaaacca attatcgtca gctgcggctc
    22981 tggtgtaacg gcagccgtgg ttttgttagc actcgcgacg ctggatgtgc caaacgtgaa
    23041 actgtacgac ggcgcatgga gtgaatgggg cgcgcgggca gatttaccgg ttgagccagt
    23101 gaaataagta ttttacaggc aataaaaaac cgccgaattt ggcggttttt tattgctggt
    23161 ctggttcgcg gcctttccag caggttgtat taccgtagta atgcaagcgc gtctcagcgg
    23221 agacaatact cgccagtaac tctctttttg tcaagcaaaa gagagttatt attgttctgt
    23281 tagtatatta tccactgcgg ccctttccgc cgtctcgcaa gcgggcgctg gcttaaggaa
    23341 aggatgttcc gcgaccgtta gagcaagcgc gtctcagcgc ttgctatcgc ggcaatatcg
    23401 ccagtggtgc tgtcgtgatg cggtcttcgc atggaccgca caatgaagat acggtgcttc
    23461 tgtatcgtac ttattgtttc tggtgcgctg ttttcccaag taaataataa ccggagtctc
    23521 tccggcgaca atttactggt ggttaacaac cttcagagca gcaagtaagc ccgaatgccg
    23581 ccctttaggg cggcatattt tagattatcc ggttttgttt aaagtcacgc aaaaaacctc
    23641 cccagcgacg ttcatagaat ggcacaatat gttcggtaat aaagtggcta attccttttt
    23701 cccctttttt cacctgacaa atatcgattg gttcatcgcc aggtaatgta tcggtcgcta
    23761 cacttcccgt cgcctgaata atttcttcga tatcaccatc ggcttcaatg ccaataaata
    23821 aattaggctg tgcctcttcg ttctctttaa ttgaacaaat aaaagcacgc ttcaccggct
    23881 taatggtttt aaataaggtg gtgagtgaat caatcatttg tgctggcggc tctgcaactt
    23941 ccgataatat cagcgattca ccgccttcca ggatttcctg gctgctcagc ggatttccct
    24001 cttcaccaat caacaaactg atttcacgcg gcataaattc tttaccggtc ggcagtttgg
    24061 cattaaggaa gagcgtttcg ccaagggtca tctcaaacaa tgtgcgaacg ggcattacga
    24121 caaatgcctg ttcgtcttca accgcctgtt gaagcgcttc taacgaggtg aaaaaaggaa
    24181 tgacgctggt gccgtcttct ttttcccagt gctgtaaatc aagcgcacta tcttcaacca
    24241 cagcctcgcc ctgcgccgcc gtaccaggca cccagacggt ggattccagt agagtacgga
    24301 aaaaggccgg gcggtgtgcc ggttcggttg ctgctttttc cagcaggtct tcaagttcgt
    24361 tttttgtttc ggacataaga accacaattc attcaacgtt cggggcgcaa atgcctgatg
    24421 cgctacgctt atcaggccta caagggattc gcaatttgtt gaatttgcag aatttgtagg
    24481 ccggataagg cgtttacgcc gcatccggca tattagttta cgccgttaac agattagcta
    24541 tcgtgcgcac accaagtccc gtagcgcccg cagaccactg ttcaaccggc gctttacggt
    24601 aagtcgccga gcagtcgata tgcagccagc cttgctgata gttctcaaca aagtgcgaca
    24661 ggaagcccgc cgccgtgctc gcgcctgccg gatacgccgc gcttccggta ttgttcagtt
    24721 cggcaaagtt agacggcagc tggctgcggt ggaattccgc cagcggcaga cgccagaatg
    24781 gttcgttctc ttgtgaggca ctcgccagca aacgaccggc aagcgcatcg tcaaaactga
    24841 acagcgcgtg ataatcatta cccagcgcag ttttcgccgc cccggtgagg gtcgccgcat
    24901 caatgatcat ttccggtttc tgcgcactgg catcaatcag accatcggca agcaccagac
    24961 gcccttcggc atcagtgttc atcacttcaa cttttttacc gttgcgatag gtgatgatat
    25021 cgcccagctt gaacgcattg ccgctaatca ggttatccgc acagcagagg aacagcttca
    25081 cgcgcttgtt cagtccgcgc gtaatggcaa atgccagcgc cccggtaacc gttgccgcgc
    25141 cgcccatgtc cgacttcatc gagtccataa acgcagtctg tttgatgctg tagccgccgg
    25201 agtcaaaagt gataccttta cctaccaggc acgcgtacac tggcgcttct ttatcgccag
    25261 ttgggttgta atccagcgcc agcaataccg gagaacgttc tgaagcgcgt ccgacggtgt
    25321 gcagccccat ataaccttgc tcacgcagat cttcgccttt ggtgatccga taagtcacac
    25381 gatcgcccgc gacgttgctg atcagatcaa cagcacgctg tgccagttgc gatggtccca
    25441 attcttctgc cggtgcgttg atggtgtcac gcacccagtc gatgatcatc aggcggttat
    25501 ccagttcctg gcgctgggca tcgtccagat ccggccactc cactttacgc gtgcctttcg
    25561 gggctttgta accttgccag aatgcccagc agcgatccgc atcccagcct tcaccgctta
    25621 actgaacatg cttgatgccc agaccgtcaa tcttgcgcgc cgcacgctgg atcagcccca
    25681 gatcgtctgc cccgttcaaa tgcagggtaa tgccgtcatt attaatgctg taagttgctt
    25741 tttctcccca gcgcgcgtcg gcaggttggg tagagagggt aatcttcatc gcttctgtca
    25801 ttttagttat ccttctttgt aaaagggccg ccagttggca gccccaggat ttttccgcta
    25861 aatgattcgc gttgcaggaa ggcggcaagt gagtgaagcc ccaggagcat agataactat
    25921 gtgactgggg tgaacgagcg cagccaacgc atctgcggcg tgaagcatga cgcggaaatt
    25981 attcggcctc gtccagccag actaacaaaa tcgcttcgag gattttctcg ttggatgcct
    26041 gcgggtcgtc gtcgaaatct tccagatcgc aaatccactg atgcatatcg gtgaatcgaa
    26101 ccgttttcgg atcaagatca ggatacgcat cgtacagtgc ttcgccaatt tcgcggctat
    26161 cggtccactt aagtcccata ctaacctctg ttaatgctca cgcgcatggt tgatagtgta
    26221 acgcgggatt tcgactacta aatcttcgtc ggtaacgcgt gcctggcagc ttaaacggct
    26281 ttcgggctcc agtccccagg ctttgtccag catgtcgtct tcctgctctg agctttctgg
    26341 cagcgagtca aaaccttcac gaacgatgca gtggcaggtg gtgcaagcac aggatttttc
    26401 acaggcgtgt tcaatctcga taccgttacg cagcgctgcg tcgagaatgg tttcaccgct
    26461 attagcttcc agaacagcgc catcagggca gaggtcctga tgaggcaaaa taacaatctt
    26521 tggcatatta aacctcgtcc acggaatggc ctttcagcgc acgacgaacc gactggtcca
    26581 tgcggcgagc ggcgaaatcc tgggtttgtt tgtctacgtt tttaatcgct tgttcgatgg
    26641 cgtcaacatc atcgccctga gccacttcac tcaggtgagc ggcagcattg tcaatgacct
    26701 gacgctctgc ggcgcttaac agcgcggcat cagcagccag cgcgccgtgc agactttcca
    26761 gcacacgcgc cgcttctact ttttgttctg ccagcatacg ggcttttacg tcctgctcgg
    26821 catagctcat tgaatctttg atcatcgaag cgatttcgct atcggtcaga ccgtaagaag
    26881 gtttgacctg aatagacgcc tcaacgccgg tggatttctc catcgccgtc acgctcaaaa
    26941 gaccgtcggc atcgacctgg aacgtcacgc gaatatgcgc accgccagcc ggtagcgccg
    27001 gaataccacg cagcgcaaaa cgcgccagtg agcggcagtc ctgcaccagt tcgcgctcac
    27061 cctgcattac atggatagac atcgccgtct gaccatcttt aaaggtggtg aaatcctgag
    27121 cgcgggccac cggaatagtg gtattacgcg gaatcacttt ctccaccagg ccgcccatcg
    27181 tttcgaggcc cagcgacagt gggatcacat caagcagcag catttcgctg tccggcttgt
    27241 tacccaccag aatatccgcc tgaatcgccg cgccaatagc gacgacttta tccgggtcga
    27301 tggaagtcag cggtggacga ccgaaaaatt cgcctacccg ttcacgcacc agcggcacgc
    27361 gagtagaacc gccaaccatc accacttcca gcacttcatc agcttctacg cccgcgtctt
    27421 tcagcgcacg acgacaagcc agtaaggttc gttttaccag tggcgcgatc agttcattga
    27481 attgttcacg gctgatttcg ccctgccagc ccgcaacgtt aacggtcacg gagtccgcat
    27541 cgctcagcgc gattttggct gcaatggcgg catccagcag ttcacgctga acgcggttat
    27601 cgctacgatc aggaatgtcc gcctgctcgc gaatgtaatc cgccagcaga tggtcgaaat
    27661 catcgccgcc gagcgcggaa tcaccgccgg ttgccagcac ttcaaacacg ccgcgactta
    27721 agcgcagaat ggaaatatca aacgtcccgc caccgaggtc ataaacggcg atcacgcctt
    27781 cctgaccgga atccagcccg taggcgatag ccgcagcggt cggttcgtta agtaagcgca
    27841 gaacatgcaa ccccgccaga cgcgccgcgt ctttggtgcc ctgacgctgg gcatcgtcaa
    27901 agtacgccgg aacggtgata acgacaccat ccagctcgcc tgccagggct tcagttgccc
    27961 gcgccgccag tgctttgagg atgtccgcag aaacgcgcac cgggttcagc agccccgccg
    28021 ccgtttcaat cattggcagg ccgttttcgc tggcctggaa ttgataaggc aaatgcggat
    28081 agcgttgctg gatatcagcc agcgagcgtc ccatcaggcg tttaacagaa ctaatggtgt
    28141 tggcggtatc gagcgctgcg ttagtacgcg cgtcataacc caccgaatgc ccttgctgtt
    28201 gatagtgaac aacagatggc agcaggtgac ggccttcatg gtcggctaac gtttcggcct
    28261 gaccgctgcg cactgtcgct accagcgagt tggttgtgcc caggtcaata ccggccgcca
    28321 gacgacgctg atgcggcgca gcactcaaac caggttcact aatttgtaat aaggccatgt
    28381 ttagcttcca gaaattaaaa atcgagcagt ttttcttcga gttgttcggc actgcttcgc
    28441 agtttatcga gaaaacgcag cttacgcacg gtatccgccg ccgcgtccca cgtctcgttg
    28501 tctaactgtt caaccatcaa ctgatggcgg gtatcaaaca tctttttcac acgtttgata
    28561 aagctttcca gccgcgcttc atctttcgcc tgttcgatct cgtccagttc ttcgcgcagc
    28621 tccagctgtt ccatcaggaa cgcggtgtcg cgcacagtat gctgctcgct ggcgagatca
    28681 aaaccgtgca aagaaagcaa atattccgcg cgcattaacg gatgacgcag cgtttgccag
    28741 gcctggttaa tggttgcaga ttgctgtacg gcggcgagtt gttccgcctg gcttccgctg
    28801 gcgaatttat caggatgata ctgacgttgt agatcctgaa aacgcaggct cagtgcctgg
    28861 gtatcgagtt gataacgggc aggcaagcca aagagggtga agtaatccat aacattctca
    28921 gggctgcgat ccgccgcgat aacggcgaat caggtaaaac aaaccccacg cgcaggcgac
    28981 cacggtgggg ttatcggtat gcgcatcaaa cgtggaagct ttcgccgcaa ccacactcat
    29041 ctttaacgtt cgggttggtg aatttaaacc cttcattcag gccttctttt acgaagtcca
    29101 gctgcgtacc gtctagaaat tgcaggcttt tgccatcgac cacgactttc acgcctttgt
    29161 cttcaaacac gatgtcttcc ggcgtcggtt cgtcaacaaa ttccagtaca taagccatac
    29221 ctgaacatcc ggaggttctc acgcccagac gcaggccaaa ccctttgccg cggttagcca
    29281 gaaaggtatt tactcgcgct gctgcactgt cgctcagtgt aatcgacata accaaacctc
    29341 aactcttatt ttgcttcacg tttgctttta tagtccgcaa tggcggcttt gatcgcgtct
    29401 tctgccagaa tagaacagtg aattttcacc ggcggcagtt caagttcttc agcaatatcg
    29461 gtgtttttga tcgcctgcgc ttcgacgaga gacttccctt tcacccattc ggtgaccagg
    29521 gagctggaag cgatagcaga accgcagccg taagttttaa aacgcgcgtc ttcaatgata
    29581 ccttcatcgt tgactttaat ctgcaacttc atcacgtcgc cacaggccgg tgcacccacc
    29641 atgccgctgc cgacgttctc gtcgttgttg tcaaaggaac ccacgttacg cggattctcg
    29701 taatggtcga taactttttc gctgtaagcc attataaatt ctcctgattc cgataccgat
    29761 taatgatgag cccattcgat gctgttcaga tccacgccct gcttgtacat ttcccacagc
    29821 ggagaaaggt cacgcagacg accgatggat ttacgaacta actcgatggt gtagtcgatc
    29881 tcttcttcag tagtaaaacg acctaaagag aaacggatag agctatgtgc cagctcgtcg
    29941 ttcagcccca gcgcgcgcag cacgtaggac ggttcgaggc ttgctgacgt acaggcggaa
    30001 cctgaagaaa ctgcgaggtc tttcagcgcc ataatcagcg actcaccttc aacgtagttg
    30061 aagctgacgt tgagaatgtt cggcgcaccg tgttccaggt caccgttcag gtaaacttct
    30121 tcgatatctt tgatgccgtt ccacagacgg ttacgcaggc cgcgcagacg ttccatctcg
    30181 gtcgccatct cttcttttgc gatgcgatag gcctcgccca ttccgacgat ctggtgaaca
    30241 ggcagagtgc cggaacgcat accgcgctcg tgaccgccgc cgtgcatttg cgcttcgatg
    30301 cgtacgcgcg gtttacgacg tacatacagc gcaccgatac ctttcgggcc atagattttg
    30361 tgaccggaga aagacatcag gtcaactttc aactggctca ggtcgatagg cagtttgccc
    30421 acgctctggg ttgcatcaac gtgatagata atgccacgag cacggcacat ttcgccgata
    30481 gccgcgatat cctgcaccac gccgatttcg ttatttacgt gcatgatgga aacaaggatg
    30541 gtgtcgtcac gcatcgctgc ttcaagttct ttcaggtcga taatgccgtt acgctgcggt
    30601 gccaggtagg tgacttcaaa accttcgcgc tccagctgac ggcaggtatc cagtaccgct
    30661 ttgtgttcgg ttttgctagt gatgatgtgc ttgccttttt tctgataaaa gttggctgca
    30721 cctttgatcg ccaggttgtc agattcggtt gcaccagagg taaagacgat ttcacgcgga
    30781 tcagcgccga ccagatcggc aatctgatta cgggcgatat ctaccgcttc ttcagcctgc
    30841 cagccgaaac ggtgagaacg agaggccggg ttaccaaagg ttccgtccat cgtcatgaac
    30901 tgcatcattt tctcggcaac acgcgggtcc accggcgtgg ttgcggagta gtcgagataa
    30961 atcggtaatt tcattgctct ataaactccg tacatcactc aatgcaagga atcaggctac
    31021 cggctggatg tacgaccgtg tttacggagt atttagcact ccggcctgat tctgaattct
    31081 ttttattaag cgcgtaactt aacgtcgatc gcgtcttgtg tgcgggtgcg tggcgcgtcg
    31141 tgagtatgct gacgaccaga cacatccagc acttcctggt tattaaccag ttcgcctaaa
    31201 gtaatgttgt tgagaaaacc ggtgagacgg tcgctcaaat cacgccacag cgcgtgggtc
    31261 aggcatttat cgccgccctg gcagccgcct ttaccctgac aacgggtggc atctacagat
    31321 tcgtcaacgg cgctaatcac ttcgccaacg gcgatgctgc tggcatcttt gcctaacaga
    31381 taaccaccgc ctggtccacg tacgctggaa accagaccat ttttacgcag acgggaaaac
    31441 agttgttcca gataagaaag ggaaatgccc tgacgttcgg aaatatcagc caacggtacc
    31501 gggcccgctt cagagttgag cgcaacgtca agcattgcgg tcacggcata gcgcccttta
    31561 gatgtcagtc tcatgtctta cttcacctca aactcgcccc tgcccggggt tttttattgt
    31621 aaaagtgggg gtattgaata gcagggtcaa gtctgacatt cccgagtaaa ttgatcaact
    31681 atttacttga ctgatttagt cgggtattta accttcagtg ccattttttt atcgtggcgt
    31741 ttgtacctgt tgtcggatgc ggtgtgaaca ccttatccga cctacggttc tgtacattgt
    31801 aggcctgata agacgcgtta agcgtcgcat caggcaacta ctgttattcg gccttgttac
    31861 ctttattctg ctgctcaata gaagccagaa tcccgcgcag gatattcaat tcctggcttt
    31921 ccgggcgcgc acgggtaaac agacggcgca atttattcat cacctgcccc ggatggtttt
    31981 cacggataaa accggttgcc agcagcgttt gttccagatg accataaaaa cgctccagat
    32041 catcgaccag cggatacggc gtctcttcat gctcgacctg ctcgccgttt tcctgagttg
    32101 ccagccaggc catgcgcact tcataggcaa taacctgaac cgccatcgcc aggttcagcg
    32161 agctgtattc cgggttagcc gcaatcgcga catgataatg gcatttctgc aactcttcat
    32221 tggtcaagcc gacgcgctcg cgaccaaaca ccagcgccac cggggtattt gccgcttcag
    32281 cgacgctttt caggccgcat tcgcgcgggt cgagcatcgg ccatggcagc gtgcgggaac
    32341 gtgcgctggt gcccaccacc aagctacaac cggctaacgc ttcgtcgagc gtatcgacga
    32401 tgtgagcatt accaatcaca tcgctggccc ctgctgccag ggcaatcgcc tgggagtcgg
    32461 gtttcaccag tggattaacc agccacagat tggttaatcc cattgttttc atggcacggg
    32521 caacagaacc catattgccg gtgtgtgacg tctccaccag cacaattcga atattttgca
    32581 gcattgtctt tcttcatcta aagattattc acgcatctta tcataaaacg aagacagatg
    32641 ccgatctcgc tgctatactc tgcgccgttt tcccgttctt taacatccag tgagagagac
    32701 cgatgcatcc gatgctgaac atcgccgtgc gcgcagcgcg caaggcgggt aatttaattg
    32761 ccaaaaacta tgaaaccccg gacgctgtag aagcgagcca gaaaggcagt aacgatttcg
    32821 tgaccaacgt agataaagct gccgaagcgg tgattatcga cacgattcgt aaatcttacc
    32881 cacagcacac catcatcacc gaagaaagcg gtgaacttga aggtactgat caggatgttc
    32941 aatgggttat cgatccactg gatggtacta ccaactttat caaacgtctg ccgcacttcg
    33001 cggtatctat cgccgttcgt atcaaaggcc gcaccgaagt tgctgtggta tacgatccta
    33061 tgcgtaacga actgttcacc gccactcgcg gtcagggcgc acagctgaac ggctaccgtc
    33121 tgcgcggtag caccgctcgc gatctcgacg gtaccattct ggcgaccggc ttcccgttca
    33181 aagcaaaaca gtacgccact acctacatca acatcgtcgg caaactgttc aacgaatgtg
    33241 cagacttccg tcgtaccggt tctgcggcgc tggatctggc ttacgtcgct gcgggtcgtg
    33301 ttgacggttt ctttgaaatc ggtctgcgtc cgtgggattt cgcggcaggc gagctgctgg
    33361 ttcgtgaagc gggcggcatc gtcagcgact tcaccggtgg tcataactat atgctgaccg
    33421 gtaacatcgt tgctggtaac ccgcgtgttg tgaaagccat gctggcgaac atgcgtgatg
    33481 agttaagcga cgctctgaag cgttaatgac tcaggcgggt gatgtcactc acccgccctc
    33541 gcctttcagg cgctattccg aaatacttcc tcaccgcttt actttctttc gtcactctcc
    33601 caccatcttt ccccgattaa tggatgataa agaagtcaca aggaaaggtt atggcgctgc
    33661 cagtgaacaa acgcgttctc aaaattctgt ttattctctt tgttgttgcc ttctgcgttt
    33721 atttagtgcc gcgcgttgcc atcaacttct tctattatcc tgacgataaa atttacggtc
    33781 ccgacccctg gtcggcggaa tccgtcgaat ttacggctaa ggacggtact cgtctgcaag
    33841 gctggtttat tccttcttcg acgggccctg ctgacaatgc catcgcaacc atcattcatg
    33901 ctcacggcaa tgccggaaat atgtccgccc actggccgct ggtcagttgg ttacccgagc
    33961 gtaatttcaa cgtttttatg tttgattatc gcgggtttgg taaatcaaaa ggcacgccgt
    34021 cccaggccgg attgctggac gatacgcaaa gtgccatcaa tgtggtgcgc catcgcagtg
    34081 atgtaaaccc acaacggctg gtgctgttcg ggcagagtat tggcggggcg aatattcttg
    34141 ctgttattgg tcaggatgat cgtgaaggca tacgtgcggt gatcctcgac tccacatttg
    34201 cctcttatgc aaccatcgcc aatcagatga tccccggcag tggctactta cttgatgaga
    34261 gttacagcgg cgaaaattat atcgccagcg tcagcccgat cccgctttta ctcattcacg
    34321 gtaaagctga tcacgttatc ccatggcagc acagcgaaaa gttgtatagc ctggcaaaag
    34381 agacaaaacg gttgatccta atcccggatg gcgaacacat tgatgctttt tccgatcgtc
    34441 acggcgatgt ttatcgcgaa cagatggtgg actttatcct tagcgcgttg aatccgcaga
    34501 actaacccat gatcgctagc acgataatca ttcacaaaac caccttaaga catgctaatc
    34561 cactggtcag aacagtttaa gatgagaaaa attctgtgac gcttgccaac atttctgatg
    34621 attagcattc ccttcgccat ttacctgagc aaactttagc tattcttatc aattatgctt
    34681 atgggagatc tacagatgat gcctacgctt gctccaccat ctgtcctttc ggctccccag
    34741 cgccgctgcc agatcttgct gacgctcttt cagccggggt tgaccgccac catggcaacc
    34801 ttcagcgagc ttaatggtgt ggatgatgat attgccagtc ttgatatcag cgaaacagga
    34861 cgggagatcc tgcgctatca tcaactcaca ctgacgactg gttatgacgg tagctaccgg
    34921 gttgaaggta cagtgcttaa ccaacgtttg tgtttatttc actggctacg acgtggtttc
    34981 cgtctgtgtc cgtcatttat taccagccaa ttcacccccg ccctgaagag tgaactgaag
    35041 cggcgcggaa ttgcgcgtaa cttttacgac gataccaatc tacaagcgtt agtgaatctc
    35101 tgctcccgac ggctgcaaaa acgctttgaa acgcgcgata ttcatttcct gtgtctgtat
    35161 ctgcaatatt gtttgctgca acaccacgct ggaattacgc ctcagtttaa tccgctccaa
    35221 cgtcgctggg cggaatcctg ccttgaattt caggtagcgc aggaaattgg acgccactgg
    35281 cagcgtcggg cgctccagcc tgtaccgcct gatgagccac tgtttatggc attacttttt
    35341 tccatgttgc gggttcccga tccattgcgt gatgcgcatc agcgagacag acaattgcgt
    35401 cagtctatca aacgtctggt aaaccatttt cgtgagctgg gaaatgttcg tttttatgat
    35461 gaacaggggt tatgcgatca gctttatacc cacctcgccc aggcgttaaa tcgcagtttg
    35521 tttgccatcg gtattgataa taccctgcca gaagagttcg ccaaactgta cccacgcctg
    35581 gtgcgcacca cccgcgcggc gctggccgga tttgaaagtg aatacggcgt ccatctttct
    35641 gatgaggaaa gtggtctggt cgcggtgatt ttcggtgcct ggctaatgca ggaaaacgat
    35701 ctgcatgaaa aacagattat tcttcttacc gggaatgata gcgagcgaga agcgcagatt
    35761 gagcagcagt tacgcgaact aacgttactg ccgctcaaca ttaaacatat gtcggtaaag
    35821 gcatttttgc agacaggcgc tccgcgcggc gcggcactga ttattgcgcc ttataccatg
    35881 ccgttaccgc tcttttcacc accgctgatc tatacggacc tgacactgac aacacatcaa
    35941 caggagcaga tccgcaaaat gctcgaatca gcatgaggga acaactttcg ggcgcaaaaa
    36001 cattgccgga agcgccacca gcgccattac ccagaacacg ccgtggccca gatattgata
    36061 caggaaaccg gcgaaaacgg tcatgatagc tatactgccg cccatcgcga cggcagagta
    36121 aaccgcctgt aaacggatga cttcgctacc ctggcgagcg gcaatatagc gcatggcggc
    36181 caggtggcag accgtgaagg cgccgcaatg cagaatttgc accactatca accacggcaa
    36241 cgccgtagtt gctcccataa tgccccagcg cactacgccg caaatcgccg agatcaacag
    36301 catatcgcgt gcactacaac ggcggaaaag tttattactc agcgcaaaga taatgacttc
    36361 cgccaccacg cccagcgacc acaaataccc caccgccgag gccgagtagc cagctgcctg
    36421 ccagtaaatg gcgctaaaac cgtaataggc cgcatgtgcc ccctgcaata aacaaacgca
    36481 ggccagaaag cgccagttct gaagaaccag cgccaaccac gctgaccaac cggtgctctc
    36541 ctgctggcgg cttgcccctt gtggctgaat cgtcggacgg atgagaaagc cgagcagcat
    36601 agatgccacg cccaatgtta acagcgcgag gatcacctga taatcaaaca tagtgaccag
    36661 tttgcccgtc agcgccgagc caatgacaaa cgccaccgag ccccacagtc gcactttgcc
    36721 gtaatcaagc gggaactgct tttgccacgt attcgccagt gcatcggtca acggtaccag
    36781 cggtgagaaa aagaggttaa agccaatcat caccagcatc agccacgcta cgtgcgcccc
    36841 cgcccagaag gcgacagcaa agagaagtgt catcattgcc agcacgcgca aggcggaaat
    36901 caggcgagaa ggatcgctga cgcggggcgc gatgagcaaa ctcccgagaa aacgggcaac
    36961 cagacctgcc cccaataaca ggccgatggt ttctggcgtt aaaccaatcc ctttaagcca
    37021 gacgctccag aaaggtagaa aaatgccgta actaaaaaag tatgtgaaat agccgagcgc
    37081 caaccagcgc gtggattgca aaaccatgag tccctcccgt caggaggcgt tagtctgggg
    37141 ttaatcgaat gttggtgcaa gttgaaaaat aagaatattc gagcctgctt cccgatgtct
    37201 gtaacgccag ttcacaagca ctaaaaaact gcgatccgcg acgcatgttg caaaaataat
    37261 gcgtaattat gccgttacgc ttgccaaacg ttcctgcaca atggcgatga aatcgcgtaa
    37321 tgccggcttc atctcccctt tcttccacgc catcagtaaa gcaatggaag gcacatttcc
    37381 ggcaatggga cgaaaaacaa cctgtccggt attaaaatta ttcatataac cgggtatcaa
    37441 agtgacgccc agccccatgc ccaccagatt catggtcacc agaatattcg ttgccacctg
    37501 gacgatattt ggctggctgt tttcttgcgc aaaccacgct ttaacgatcg gcgcaagcga
    37561 accggaatac gccggatcgg tactgacgaa attcacgcca tccagttgcg cggcggtgat
    37621 ctctttttca tgtgctaaag ggtgatcaac cggtaacaca accactaatg gttcgtcaaa
    37681 aagctccaga taatcaatct ccgggctata aacaggatgg cgcattaagc cgacgtcgag
    37741 ttcaccacgg cgaatttttt cctcctgttg cgtggtgatt aaactcacca gctcaatcaa
    37801 ggtgtctggc tgtctgagac gaaacatcgg taatactttt ggcagtaaat tcacttccgc
    37861 cgatggcaca aagccaatgg ttaattgtct gtcttcctga acagttttcc gcgcccgtaa
    37921 tttggcattt tccgcttgtt cgaggattgc cagcgcgtcc tggagaaaac attctcccgc
    37981 cgccgtcaac gcgactttgc gcttatccct caccagtaac ggaacaccga cacagttttc
    38041 aagatcgcgg atctggctgc ttaacgaagg ctgtgaggta tgcagttttt ccgccgcacg
    38101 ggtaaagttc agtgcctgcg ccactgcgac gaaatagcgt aaatgccgta gttccatcac
    38161 cttccccttg ttatcgaaaa aacgtctcag ctggtagaaa ttaactattt cacatattag
    38221 caaccaacca gcaacatcct tatggcacaa aaatagaagg tcaatacatc ttatctttca
    38281 ggattaaaaa atatgaccac accctcagat ttgaacattt accaactgat tgacacccaa
    38341 aatggtcggg tcactccgcg tatttatacc gacccggata tttaccaact ggagcttgag
    38401 cgtattttcg gtcgttgctg gctatttctc gcccacgaaa gccagatccc aaaacccggt
    38461 gatttcttta acacctacat gggagaagat gcggttgtcg tagtgcgtca gaaagacggc
    38521 agcatcaagg cgtttctcaa ccaatgccgc caccgggcca tgcgtgtgag ttatgcagat
    38581 tgcggcaaca ctcgcgcctt tacctgcccg tatcacggct ggtcttatgg cattaacggc
    38641 gagttgatcg atgtaccgct ggaacctcgc gcctacccac aagggttgtg taaatcccac
    38701 tgggggctaa acgaagttcc ttgtgtggag agttataaag ggctgatttt tggcaactgg
    38761 gataccagcg caccgggcct gcgtgattac cttggtgaca ttgcctggta tctggatggc
    38821 atgctggatc gtcgcgaagg cggcaccgaa attgtcggcg gcgtacaaaa gtgggtgatc
    38881 aactgtaact ggaaatcccc ggcagagcag ttcgccagtg accaatatca tgctctgttc
    38941 agccatgctt ctgccgttca ggtattaggg gcgaaagatg atggcagcga taagcgtctc
    39001 ggtgatggac aaaccgcgcc cggtgtggga aaccgccaaa gatgcgctgc aatttggtca
    39061 ggacggtcac ggtagcggtt tcttctttac tgaaaaaccg gatgctaatg tctgggtcga
    39121 tggcgcggtt tccagctatt accgcgaaac ctatgccgaa gcagaacaac gtttaggtga
    39181 agttcgcgcc ctgcgcctgg cgggtcataa caatatattc cccacgcttt catggctaaa
    39241 cggcactgcc acactccgcg tctggcatcc gcgcggccct gatcaagttg aagtgtgggc
    39301 gttctgtatt actgacaaag ccgcctccga tgaagttaaa gccgcttttg aaaacagcgc
    39361 cactcgtgct tttggtcctg ctggttttct cgagcaggat gactcggaga actggtgtga
    39421 aatccagaaa ttgcttaaag gccaccgcgc ccgcaacagc aaactgtgtc tggaaatggg
    39481 gcttggtcag gaaaagcgcc gcgacgacgg cattcctggc attactaact atatcttttc
    39541 agaaactgcc gctcgtggaa tgtaccaacg ctgggccgat cttctgagta gcgaaagctg
    39601 gcaggaagtg ctcgataaaa ccgccgctta ccagcaggag gtgatgaaat gagtgcgcaa
    39661 gtttcactag agttacatct ccgcattagc cagtttctct ttcacgaagc cagcttactg
    39721 gacgactgga aatttcgtga ctggctggcg cagctcgacg aagagattcg ttacaccatg
    39781 cgcaccacag ttaacgcgca aacacgcgac cgccgcaaag gcgtccagcc accgacaacc
    39841 tggattttta atgacaccaa agaccagctg gagcggcgaa tcgcccgtct ggaaacgggc
    39901 atggcctggg cagaagagcc gccgtcacgc acccgtcact taatcagcaa ctgccagata
    39961 agcgaaaccg acatcccaaa cgtatttgct gtgcgggtaa attatctgct ttatcaggca
    40021 caaaaagagc gcgatgaaac attctatgtt ggaacgcgtt tcgacaaagt tcgccgtctg
    40081 gaagatgaca actggcgctt gctggaacgg gatatcgtcc tggatcaagc ggtaatcact
    40141 tcccataacc tgagtgtact gttctgatga atcgaattta tgcgtgtccc gttgcggatg
    40201 tgccggaggg tgaggctctc cggatcgata cctcgcccgt catcgccctg ttcaacgttg
    40261 gcggcgagtt ttatgccatt aacgatcgtt gcagccatgg taatgcgtcg atgtcagaag
    40321 ggtatctgga agatgacgcc acggtggagt gcccgctaca cgccgccagt ttttgcctga
    40381 aaacggggaa agcgttatgc ctgcccgcca ccgatccgct caccacttat ccagtacacg
    40441 ttgaaggtag tgacattttc atcgacttac cggaggcgca gccatgagcg atctgcataa
    40501 cgagtccatt tttattaccg gcggcggatc gggattaggg ctggcgctgg tcgagcgatt
    40561 tatcgaagaa ggcgcgcagg ttgccacgct ggaactgtcg gcggcaaaag tcgccagtct
    40621 gcgtcagcga tttggcgaac atattctggc ggtggaaggt aacgtgacct gttatgccga
    40681 ttatcaacgc gcggtcgatc agatcctgac tcgttccggc aagctggatt gttttatcgg
    40741 caatgcaggc atctgggatc acaatgcctc actggttaat actcccgcag agacgctcga
    40801 aaccggcttc cacgagctgt ttaacgtcaa cgtactcggt tacctgctgg gcgcaaaagc
    40861 ctgcgctccg gcgttaatcg ccagtgaagg cagcatgatt ttcacactgt caaatgccgc
    40921 ctggtatcct ggcggcggtg gcccgctgta caccgccagt aaacatgccg caaccggact
    40981 tattcgccaa ctggcttatg aactggcacc gaaagtgcgg gtgaatggcg tcggcccgtg
    41041 tggtatggcc agcgacctgc gcggcccaca ggcgctcggg caaagtgaaa cctcgataat
    41101 gcagtctctg acgccggaga aaattgccgc cattttaccg ctgcaatttt tcccgcaacc
    41161 ggcggatttt acggggccgt atgtgatgtt ggcatcgcgg cgcaataatc gcgcattaag
    41221 cggtgtgatg atcaacgctg atgcgggttt agcgattcgc ggcattcgcc acgtagcggc
    41281 tgggctggat ctttaaggaa gcacgatgaa agaaaaaacg atcattattg tcggtggcgt
    41341 gcaagcggcg gcaatggctg cggcctcgct acgccagcaa gggttcaccg gtgagctgca
    41401 tctgttttcc gatgagcgac atcttcctta tgaacgacct ccactctcga aatccatgtt
    41461 gctggaagat tccccgcagt tacagcaggt gttacccgct aactggtggc aggaaaacaa
    41521 tgttcatctg cattccggtg taaccatcaa aacgctgggt cgcgacacac gagagttagt
    41581 gttaaccaac ggcgaaagct ggcactggga tcagcttttt atagcaaccg gcgcggcagc
    41641 tcgaccgctg ccgttgcttg atgcactggg agaacgctgc tttaccctac gccatgccgg
    41701 tgatgccgcc agactgcgag aagttctgca gcccgaacgg tcagtcgtga ttgtcggtgc
    41761 cggaactatt ggtctggaac tggctgccag cgccacgcag cgcagcgcag cgcagcgcag
    41821 atgtaaggtg acagtgattg aactggcggc aaccgtcatg ggccgtaatg caccaccgcc
    41881 cgtgcaacgc tatcttttac agcgccacca gcaggctggt gtgcgcattc tgctcaataa
    41941 tgccattgaa catgtagtcg atggtgaaaa agtagaactg acgctgcaaa gtggggaaac
    42001 gcttcaggct gatgtggtga tttacggtat tggtatcagc gccaacgagc aactggctcg
    42061 cgaggccaac cttgatactg ccaatggcat tgtcattgat gaggcttgcc gcacctgcga
    42121 tcccgcgatc tttgccggtg gcgatgtggc aatcactcgt cttgataatg gtgcactaca
    42181 ccgctgtgaa agctgggaaa acgccaataa ccaggcgcaa attgccgctg ccgcaatgtt
    42241 agggctaccg ctactgccgt ggttctggag cgatcagtac agtgataact tacagtttat
    42301 tggcgatatg cgtggcgatg actgaggtag cctgagttta acggacactc cttcctgaaa
    42361 tagaatggca tcatgaagga gctaataatg agcag
//
LOCUS       DADLFA010000009        40982 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000009 DADLFA010000000
VERSION     DADLFA010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40982
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.009"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..334)
                     /locus_tag="JQH65_000443"
     CDS             complement(<1..334)
                     /locus_tag="JQH65_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HAY6137959.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGE"
     gene            complement(415..1842)
                     /locus_tag="JQH65_000444"
     CDS             complement(415..1842)
                     /locus_tag="JQH65_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HAY6137960.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETEPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKQLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHLLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDDGGPGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(1839..2546)
                     /locus_tag="JQH65_000445"
     CDS             complement(1839..2546)
                     /locus_tag="JQH65_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HAY6137961.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            2709..3686
                     /locus_tag="JQH65_000446"
     CDS             2709..3686
                     /locus_tag="JQH65_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HAY6137962.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEHAIFWLKKTALQGHTFASN
                     ALGWILDRGEDPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(3756..4238)
                     /locus_tag="JQH65_000447"
     CDS             complement(3756..4238)
                     /locus_tag="JQH65_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HAY6137963.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            4473..7055
                     /gene="leuS"
                     /locus_tag="JQH65_000448"
     CDS             4473..7055
                     /gene="leuS"
                     /locus_tag="JQH65_000448"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAY6137964.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNELDKLDHWPDTVKS
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            7070..7651
                     /gene="lptE"
                     /locus_tag="JQH65_000449"
     CDS             7070..7651
                     /gene="lptE"
                     /locus_tag="JQH65_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HAY6137965.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            7651..8682
                     /gene="holA"
                     /locus_tag="JQH65_000450"
     CDS             7651..8682
                     /gene="holA"
                     /locus_tag="JQH65_000450"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAY6137966.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRTLHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8684..9325
                     /gene="nadD"
                     /locus_tag="JQH65_000451"
     CDS             8684..9325
                     /gene="nadD"
                     /locus_tag="JQH65_000451"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAY6137967.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIANKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNQED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            9349..9959
                     /locus_tag="JQH65_000452"
                     /pseudo
     CDS             9349..9959
                     /locus_tag="JQH65_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
     gene            10219..10536
                     /gene="rsfS"
                     /locus_tag="JQH65_000453"
                     /gene_synonym="rsfA"
     CDS             10219..10536
                     /gene="rsfS"
                     /locus_tag="JQH65_000453"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAY6137968.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10540..11007
                     /gene="rlmH"
                     /locus_tag="JQH65_000454"
     CDS             10540..11007
                     /gene="rlmH"
                     /locus_tag="JQH65_000454"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010618862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAY6137969.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            11038..12939
                     /gene="mrdA"
                     /locus_tag="JQH65_000455"
     CDS             11038..12939
                     /gene="mrdA"
                     /locus_tag="JQH65_000455"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HAY6137970.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTTHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            12942..14054
                     /gene="mrdB"
                     /locus_tag="JQH65_000456"
                     /gene_synonym="rodA"
     CDS             12942..14054
                     /gene="mrdB"
                     /locus_tag="JQH65_000456"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HAY6137971.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            14065..15153
                     /gene="rlpA"
                     /locus_tag="JQH65_000457"
     CDS             14065..15153
                     /gene="rlpA"
                     /locus_tag="JQH65_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HAY6137972.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSQFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            15293..16504
                     /gene="dacA"
                     /locus_tag="JQH65_000458"
     CDS             15293..16504
                     /gene="dacA"
                     /locus_tag="JQH65_000458"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HAY6137973.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDMYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16615..16878
                     /locus_tag="JQH65_000459"
     CDS             16615..16878
                     /locus_tag="JQH65_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HAY6137974.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVEILYEELGKIDIVRMVL"
     gene            16979..17620
                     /gene="lipB"
                     /locus_tag="JQH65_000460"
     CDS             16979..17620
                     /gene="lipB"
                     /locus_tag="JQH65_000460"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HAY6137975.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            17879..18832
                     /locus_tag="JQH65_000461"
                     /pseudo
     CDS             17879..18832
                     /locus_tag="JQH65_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
     gene            19041..20006
                     /gene="lipA"
                     /locus_tag="JQH65_000462"
     CDS             19041..20006
                     /gene="lipA"
                     /locus_tag="JQH65_000462"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HAY6137976.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVAIEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(20107..20310)
                     /gene="tatE"
                     /locus_tag="JQH65_000463"
     CDS             complement(20107..20310)
                     /gene="tatE"
                     /locus_tag="JQH65_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HAY6137977.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(20439..21227)
                     /locus_tag="JQH65_000464"
     CDS             complement(20439..21227)
                     /locus_tag="JQH65_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HAY6137978.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            21320..21703
                     /gene="crcB"
                     /locus_tag="JQH65_000465"
     CDS             21320..21703
                     /gene="crcB"
                     /locus_tag="JQH65_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HAY6137979.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLTANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(21758..21967)
                     /gene="cspE"
                     /locus_tag="JQH65_000466"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(21758..21967)
                     /gene="cspE"
                     /locus_tag="JQH65_000466"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HAY6137980.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(22142..22702)
                     /locus_tag="JQH65_000467"
     CDS             complement(22142..22702)
                     /locus_tag="JQH65_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid:lipid A palmitoyltransferase"
                     /protein_id="HAY6137981.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAPTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            23291..24676
                     /gene="dcuC"
                     /locus_tag="JQH65_000468"
     CDS             23291..24676
                     /gene="dcuC"
                     /locus_tag="JQH65_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HAY6137982.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(24717..25397)
                     /gene="dpiA"
                     /locus_tag="JQH65_000469"
                     /gene_synonym="citB"
     CDS             complement(24717..25397)
                     /gene="dpiA"
                     /locus_tag="JQH65_000469"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HAY6137983.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTSARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(25366..27023)
                     /gene="dpiB"
                     /locus_tag="JQH65_000470"
                     /pseudo
     CDS             complement(25366..27023)
                     /gene="dpiB"
                     /locus_tag="JQH65_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
     gene            27372..28430
                     /gene="citC"
                     /locus_tag="JQH65_000471"
     CDS             27372..28430
                     /gene="citC"
                     /locus_tag="JQH65_000471"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HAY6137984.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLKTPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            28445..28741
                     /gene="citD"
                     /locus_tag="JQH65_000472"
     CDS             28445..28741
                     /gene="citD"
                     /locus_tag="JQH65_000472"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HAY6137985.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGTLDCILRARLEALLARASGIPTLPWEDCQ"
     gene            28738..29646
                     /gene="citE"
                     /locus_tag="JQH65_000473"
     CDS             28738..29646
                     /gene="citE"
                     /locus_tag="JQH65_000473"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HAY6137986.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            29657..31189
                     /gene="citF"
                     /locus_tag="JQH65_000474"
     CDS             29657..31189
                     /gene="citF"
                     /locus_tag="JQH65_000474"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HAY6137987.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIHRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQEQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            31193..31743
                     /gene="citX"
                     /locus_tag="JQH65_000475"
                     /pseudo
     CDS             31193..31743
                     /gene="citX"
                     /locus_tag="JQH65_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
     gene            31718..32566
                     /gene="citG"
                     /locus_tag="JQH65_000476"
     CDS             31718..32566
                     /gene="citG"
                     /locus_tag="JQH65_000476"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HAY6137988.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACKGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQSLTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLALTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGACAGYSARRKHYCKKGAFEPPPISIISGSSTGSVSNEISVQ
                     AAVPTC"
     gene            32646..34109
                     /gene="citT"
                     /locus_tag="JQH65_000477"
     CDS             32646..34109
                     /gene="citT"
                     /locus_tag="JQH65_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HAY6137989.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPNGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            34220..35026
                     /gene="rna"
                     /locus_tag="JQH65_000478"
     CDS             34220..35026
                     /gene="rna"
                     /locus_tag="JQH65_000478"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HAY6137990.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPSNCGKTFVIDKVGY"
     gene            35256..35666
                     /gene="rnk"
                     /locus_tag="JQH65_000479"
     CDS             35256..35666
                     /gene="rnk"
                     /locus_tag="JQH65_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HAY6137991.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(35897..37135)
                     /locus_tag="JQH65_000480"
                     /pseudo
     CDS             complement(35897..37135)
                     /locus_tag="JQH65_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
     gene            37356..37784
                     /gene="uspG"
                     /locus_tag="JQH65_000481"
     CDS             37356..37784
                     /gene="uspG"
                     /locus_tag="JQH65_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HAY6137992.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLTQDDGVIHLLHVLPGSA
                     SLSLDRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(37905..39470)
                     /gene="ahpF"
                     /locus_tag="JQH65_000482"
     CDS             complement(37905..39470)
                     /gene="ahpF"
                     /locus_tag="JQH65_000482"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAY6137993.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(39641..40204)
                     /gene="ahpC"
                     /locus_tag="JQH65_000483"
     CDS             complement(39641..40204)
                     /gene="ahpC"
                     /locus_tag="JQH65_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HAY6137994.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            40516..>40982
                     /gene="dsbG"
                     /locus_tag="JQH65_000484"
     CDS             40516..>40982
                     /gene="dsbG"
                     /locus_tag="JQH65_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706462.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HAY6137995.1"
                     /translation="MTVIGYAFYSTFALTEKDKLMLKKILLLALLPAIAFAEELPAPV
                     KAIEKQGITIIKTFDAPGGMKGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSN
                     TLIEKEIYAPAGREMWQRMEQSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQA"
BASE COUNT        10180 a        10481 c        10073 g        10248 t
ORIGIN      
        1 cttccccaca gcacatagcg gtttgggcaa cgaattgggt ataaatcaac cctctctccg
       61 agccaccagc aattaatgct ttccactctt caggggaaga gcgatgccat gtcagatata
      121 agccagctaa atcattggca tgaccatcat cagtcatctg ataaatagtc tgaattaaat
      181 catcacgatt atttattccc caggatgatg agagatcgaa ttcttttata ccggtataca
      241 tttttggcga gtcatgcgcg ggtttgcaac cctgattaag tatttccatg ggcgcagaaa
      301 gcgcaaaaag aagatattgt tcttccttat tcatttgtta ataatccctt aagtgcatta
      361 actgactact tttttatatt acttgccggg ctttccgcac attaacgtcg actgtcaccc
      421 gaacagatgg agtattttcc ctatcaaacc ggcaaagata aaaatgtaga aaataatttt
      481 gatgacgccc agacagcccg gcccaccatc gtcaacgggc attggctgcg gttgcggctc
      541 cggttcaaca gtttgcttct cggtaaaatt cgccgcctga attaataatc gacgaataat
      601 taatgcttcc agtggacaac gatcttctgc atcaagacgg cttacgatcg gcattaatga
      661 ttcttctgtc ggttggctgg cctgcactaa ttgtttggca actaaatata atggccataa
      721 gagatgttct gtatcacgcg tgagtgagat ccgcggctta cgtcctttgt gcaaaaccat
      781 ctcgaccagt gaacgcccca gcgcagagag tttttcatcg ctgagtattt cgtataaatg
      841 aaaacgcgta cgggcgctgt cgccggggac ataaagtagc tgtttaccct gatcgttaac
      901 cagttcacga gcttcaatca tcatcaccac taacgggtaa taatcgaccc gatgctgacg
      961 gcaccattgc aatattaaag catccgcctg ggtggaggga tactcgcgcc agtagtcaca
     1021 aagttctgcc agcaagcttt cgccttctcc acaatagacg cgttgcgcgt attcataata
     1081 acgcgcatct tcattatcag gttcagcttc ccgccacgac cgggcgtaat ccagaagttc
     1141 ggggatatcc aggcgacctg cgctatacca gcggagtaaa tttctgtgca gttttgcatc
     1201 atcggggatg atcactgcgc gatgttgcgc taaatacaat gtcagccatt ctggatgcga
     1261 tgaccaaatc cgagcgaaca tttggtaaaa atcgatggtg tcattttgca cggcaacggg
     1321 cagatgcaga atggtctgga agttaaaaac attccccttc gcaatggcat ataaaaagga
     1381 ttccagttct tcttcgtcta tttccccgac gttttcctcc tgcaaccacc gcaatctttc
     1441 tgctaataac accacgcact cgaaggataa atgggcagtg ttcatggcga ttgtgcacag
     1501 cgaccagcgt aattcatcaa tctcttccat cgagcaataa tttaattgct gaatgaatcg
     1561 ctgccaggcg gagggcagaa aacgttcgct atcagaggca agtaacgcgc gaaaggcgag
     1621 cagaatttca tgttctgcta cctcatattc ttctggttgc caaacagatt tagcaggcga
     1681 ctgcgcaatc cgtagcgcct cctcatacgc ctgacgaagt tgtttaaaac cctgcggttc
     1741 ggtttctgga tgaaaggacg gtaacagcgc cagataagcg cggcggataa tatcgacgtc
     1801 agtcgtttcc tctatatcga ggatcttcca gcaatttttc ataagtcgct catatcctta
     1861 agcgtttccg ggcatgtggg gtagtcagcc agtggttgcc aggacaaggt agggtaatac
     1921 gcatcatcac tggcaaataa ttgataaaac atatcattgc cggaatcgtc gtattcttta
     1981 cgcgctaaga attcgcgtaa taaaggcaga tgatcatctt cgggagactg ccaatattgc
     2041 cgaccaaagg tataaccggc aaagtattgc cgccagttac tataataacg tagagcccgt
     2101 agatgaatac gcgactggat ccatagactt tcttcttcac tcaaccagtt attaagtacg
     2161 cccattcgac ttaaaaaacc catccggaca taatcccatg ctttaattcc tccctcacca
     2221 cagcattcgg ctgtgctggc gacaaattgc gcataagcct gaccttgctc atttaattca
     2281 gcaagatagt cacgccattc acatggagaa taacgaaacc agcggtgata aaaccctgcc
     2341 aaccgagcgg catgaccgtt ctcgatcatt cggtgaattg tgcgcagcag gtcatcacgg
     2401 tcatcaattc cccatgagga agaaaggtca aaaaagcatg tacctaaata cattttgggg
     2461 gatgaatgcg aaggtaaaca gcattcattg cgtatttcca tcggagtgga aagtgcaaac
     2521 aatatttttt gcgaccccat gtccatataa tttatcctta aacattcatt taatgaatgt
     2581 aaactacatt tcgagactca gttatttata cttttctgac gcgacataat acgcataaaa
     2641 taaggcttac gcaaaaatgg aacacataaa atataatatg ttttatctac aaccataagg
     2701 aaatgataat gatttttacg tcaagttgct gcgataattt atcaatagat gagattatcg
     2761 aacgtgctga aaaaggcgat tgcgaggctc aatatattgt tgggttttat tataatcgcg
     2821 atagcgcaat tgactctccg gacgacgaaa aggcctttta ctggctgaag ctggccgccg
     2881 agcaaggtca ttgtgaagca caatattcct taggacaaaa gtatactgag gataaaagcc
     2941 gtcataaaga taatgagcat gccatctttt ggctgaaaaa aactgcccta caaggccata
     3001 ctttcgcttc caacgccctt ggctggatac tggatcgtgg agaagacccc aattataaag
     3061 aagcggttgt ctggtatcag atagccgcgg agagcggaat gtcttatgcg caaaataatc
     3121 ttgggtggat gtacagaaat ggcaacggag tcgcaaaaga ctatgcgctg gcatttttct
     3181 ggtacaaaca agctgcatta caaggccata gtgacgcgca aaacaatctg gccgatcttt
     3241 atgaagacgg aaaaggcgtt gctcaaaaca agacactcgc cgcattctgg tatttgaaaa
     3301 gcgcacagca gggtaatcgg cacgcccagt ttcaaattgc atgggattat aacgctggcg
     3361 aaggggtgga ccaggactat aagcaagcga tgtactggta tctgaaggct gccgctcagg
     3421 aaagcgtcgg cgcttacgtc aacatcggtt atatgtataa acacggacaa ggcgttgaga
     3481 aagattatca ggctgccttt gaatggttta cgaaagccgc tgaatgcaat gacgccactg
     3541 cctggtataa cctggccatt atgtatcact acggagaagg aagacctgtc gatctccgac
     3601 aggctctcga cctgtatcgt aaagttcagt catccggaac cagagatgtc agtcaggaaa
     3661 ttcgtgagac tgaagattta ctgtagagat aaaaacaagg gccggggaat ttcattcggc
     3721 ccatattctc tttattgctg tgcgaaatca gactattacg gctcaaacgg gcgtctctgg
     3781 aactggtcat gaccacattt cgggcatagc gtcagcactt ccggtgtgta gatcgggaga
     3841 tggaagtgac atttctcgca gaccagattt cccagcccga ccacttctcc gctgtgataa
     3901 accccatgat gattgaggtc ctggaaaact tcgcgccatt caagctgcgt tttatcggtg
     3961 atatctgcca gctcctgcca taagctttct ttaatcaccc gcataaagac gctgtcagat
     4021 tcttctttca ggctctcttc atagctcatg gcgaactctt ccaggtcacg tcttacagct
     4081 cgcgtcagct catcgacctc ggttcgcgtt aactcccctg tttttattac gcgctcgcgc
     4141 gcctgttcca ccagtgcgtc gatatcacgt tcgccattgc gcaggcgttc gctcagtgac
     4201 gcgaccagtt cacggtaata ttgagcaacc ttgttcatcg tttcatctcc cgggtcgtta
     4261 actgactata ataatagacg ttctttgttc cttgctttgc taatacgccc acaaactctg
     4321 taaattaatg tatgaataat ccgggcatca tttcaacgtc gagtgcgcga aaggctgttt
     4381 tgacgcggac gtttgggcta tgctatgcgg atctgaaaaa ccacctcaac gctacatttg
     4441 tagccgtatt gaaaacagga ccactggctg ccatgcaaga gcaataccgc ccggaagaga
     4501 tagaatccaa agtacagctt cattgggatg agaagcgcac atttgaagta accgaagacg
     4561 agagcaaaga gaagtattac tgcctgtcta tgcttcccta tccttctggt cgactacaca
     4621 tgggccacgt acgtaactac accatcggtg acgtgatcgc ccgctaccag cgtatgctgg
     4681 gcaaaaacgt cctgcagccg atcggctggg acgcgtttgg tctgcctgcg gaaggcgcgg
     4741 cggtgaaaaa caacaccgct ccggcaccgt ggacgtacga caacatcgcg tatatgaaaa
     4801 accagctcaa aatgctgggc tttggttatg actggagccg cgagctggca acctgtacgc
     4861 cggaatacta ccgttgggaa cagaaattct tcaccgagct gtataaaaaa ggcctggtat
     4921 ataagaagac ttctgcggtc aactggtgcc cgaacgacca gaccgtactg gcgaacgaac
     4981 aagttatcga cggctgctgc tggcgctgcg ataccaaagt tgaacgtaaa gagatcccgc
     5041 agtggtttat caaaatcact gcttacgctg acgagctgct caacgagctg gataaactgg
     5101 atcactggcc agacaccgtt aaaagcatgc agcgtaactg gatcggtcgt tccgaaggcg
     5161 tggagatcac cttcaacgtt aacgactatg acaacacgct gaccgtttac actacccgcc
     5221 cggacacctt tatgggttgt acctacctgg cggtagctgc gggtcatccg ctggcgcaga
     5281 aagcggcgga aaataatcct gaactggcgg cctttattga cgaatgccgt aacaccaaag
     5341 ttgccgaagc tgaaatggcg acgatggaga aaaaaggcgt cgatactggc tttaaagctg
     5401 ttcacccatt aacgggcgaa gaaattcccg tttgggcagc aaacttcgta ttgatggagt
     5461 acggcacggg cgcagttatg gcggttccgg gtcacgacca gcgcgactac gagtttgcct
     5521 ctaaatacgg cctgaacatc aaaccagtta tcctggctgc tgacggctct gagccagatc
     5581 tttctcagca agccctgact gaaaaaggcg tgctgttcaa ctctggcgaa ttcaacggtc
     5641 ttgaccatga agcggccttc aacgccatcg ccgataaact gactgcgatg ggcgttggcg
     5701 agcgtaaagt gaactaccgc ctgcgcgact ggggtgtttc tcgtcagcgt tactggggcg
     5761 cgccgattcc gatggtgacg ctggaagacg gtaccgtaat gccgaccccg gacgaccagc
     5821 tgccggtgat cctgccggaa gatgtggtga tggacggcat taccagcccg attaaagcag
     5881 atccggagtg ggcaaaaact accgttaacg gtatgccagc gctgcgtgaa accgacactt
     5941 tcgacacctt tatggagtcc tcctggtact atgcgcgcta cacttgccca gagtacaaag
     6001 aaggtatgct ggattccgaa gcggctaact actggctgcc ggtggatatc tacattggtg
     6061 gtatcgaaca cgccattatg cacctgctct acttccgctt cttccacaaa ctgatgcgtg
     6121 atgcaggcat ggtgaactct gacgaaccag cgaaacagtt gctgtgtcag ggtatggtgc
     6181 tggcagatgc cttctactat gttggcgaaa acggcgaacg taactgggtt tccccggttg
     6241 atgctatcgt tgaacgtgac gagaaaggcc gtatcgtgaa agcgaaagat gcggcaggcc
     6301 atgaactggt ttataccggc atgagcaaaa tgtccaagtc gaagaacaac ggtatcgacc
     6361 cacaggtgat ggttgaacgt tacggcgcgg acaccgttcg tctgtttatg atgtttgctt
     6421 ctccggctga tatgactcta gaatggcagg aatccggtgt ggaaggggct aaccgcttcc
     6481 tgaaacgtgt ctggaaactg gtttacgagc acacagcaaa aggtgatgtt gcggcactga
     6541 acgttgatgc gctgactgaa gatcagaaag cgctgcgtcg cgatgtgcat aaaacgatcg
     6601 ctaaagtgac cgatgatatc ggccgtcgtc agaccttcaa caccgcaatt gcggcgatta
     6661 tggagctgat gaacaaactg gcgaaagcac caaccgatgg cgagcaggat cgcgctctga
     6721 tgcaggaagc gctgctggcc gttgtccgta tgcttaaccc gttcaccccg cacatctgct
     6781 tcacgctgtg gcaggaactg aaaggcgaag gcgatatcga caacgcgccg tggccggttg
     6841 ctgacgaaaa agcgatggtg gaagactcca cgctggtcgt ggtgcaggtt aacggtaaag
     6901 tccgtgccaa aatcaccgtt ccggtggacg caacggaaga acaggttcgc gaacgtgctg
     6961 gccaggaaca tctggtagca aaatatcttg atggcgttac cgtgcgtaaa gtgatttacg
     7021 taccaggtaa actcctcaat ctggtcgttg gctaagcgcg ggaggaagcg tgcgatatct
     7081 ggcaacattg ttgttatctc tggcggtgtt aatcaccgcc gggtgtggct ggcatctgcg
     7141 tgataccacg caggttcctt ccactatgaa ggtcatgatc ctggactcag gcgatccgaa
     7201 cgggccatta agccgtgcgg tgcgtaacca gttacgtctg aatggtgtcg agttgcttga
     7261 taaagaaacc acgcgtaagg acgttccatc cctgcgtttg ggtaaagtga gcatcgcgaa
     7321 agataccgca tcggtattcc gtaacggtca aacagcagag tatcagatga tcatgacggt
     7381 gaatgcgacc gtgttgatcc ccggccgtga tatctacccg attagcgcca aagtcttccg
     7441 ttcgttcttt gataacccgc aaatggcgtt agcgaaagat aacgaacaag acatgatcgt
     7501 aaaagagatg tacgaccgtg ctgccgaaca gctgattcgt aagctgccaa gcatccgtgc
     7561 tgcggatatt cgttccgacg aagaacagac gtcgaccaca acggatactc cggcaacgcc
     7621 tgcacgcgtc tccaccacgc tgggtaactg atgattcggt tgtacccgga acaactccgc
     7681 gcgcagctca atgaagggct gcgcgcggcg tatcttttac ttggtaacga tcctctgtta
     7741 ttgcaggaaa gccaggacgc tgttcgtcag gtagctgcgg cacaaggatt cgaagaacac
     7801 cacacttttt ccattgatcc caacactgac tggaatgcga tcttttcgtt atgccaggct
     7861 atgagtctgt ttgccagtcg acaaacgcta ttgctgttgt taccagaaaa cggaccgaat
     7921 gcggcgatca atgaacaact tctcacactc accggacttc tgcatgacga cctgctgttg
     7981 atcgtccgcg gtaataaatt aagcaaagcg caagaaaatg ccgcctggtt tactgcgctt
     8041 gcgaatcgca gcgtgcaggt gacctgtcag acaccggagc aggctcagct tccccgctgg
     8101 gttgctgcgc gcgcaaaaca gctcaactta gaactggatg acgcggcaaa tcaggtgctc
     8161 tgctactgtt atgaaggtaa cctgctggcg ctggctcagg cactggagcg tttatcgctg
     8221 ctctggccag acggcaaatt gacattaccg cgcgttgaac aggcggtgaa tgatgccgcg
     8281 catttcaccc cttttcattg ggttgatgct ttgttgatgg gaaaaagtaa gcgcacattg
     8341 catattcttc agcaactgcg tctggaaggc agcgaaccgg ttattttgtt gcgcacatta
     8401 caacgtgaac tgttgttact ggttaacctg aaacgccagt ctgcccatac gccactgcgt
     8461 gcgttgtttg ataagcatcg ggtatggcag aaccgccggg gcatgatggg cgaggcgtta
     8521 aatcgcttaa gtcagacgca gttacgtcag gccgtgcaac tcctgacacg aacggaactc
     8581 accctcaaac aagattacgg tcagtcagtg tgggcagagc tggaagggtt atctcttctg
     8641 ttgtgccata aacccctggc ggacgtattt atcgacggtt gatatgaaat ctttacaggc
     8701 tctgtttggc ggcacctttg atccggtgca ctatggtcat ctaaaacccg tggaaacgct
     8761 ggcgaatttg attggtctga cgcgggtcac aatcatccct aataatgttc ctccgcatcg
     8821 tccccagccg gaagcgaaca gcgtgcagcg taaacacatg cttgaactgg cgattgccaa
     8881 caagccatta tttactcttg atgaacgcga gctaaagcgc aatgccccct cttacactgc
     8941 gcaaacactg aaagagtggc ggcaggaaca aggaccggac gtgccgctgg cgtttattat
     9001 tggtcaggat tcactgctga cctttccgac ctggtacgaa tacgaaacga tactcgacaa
     9061 tgcacatttg atcgtctgtc ggcgtccagg ttacccactt gaaatggcgc aaccgcaata
     9121 ccagcaatgg ctggaagatc atttgacaca taaccaggaa gatcttcacc ttcagcctgc
     9181 cggtaaaatt tatctggctg aaacgccgtg gtttaacatc tcggcgacca tcatccgcga
     9241 acgtttgcaa aacggtgaat cgtgtgagga tttattgccg gaaccggtac tgacttacat
     9301 taaccaacaa ggcttgtatc gctgatacct gtcgttacgg agggcatgat gcgactgtgg
     9361 ttaattcgtc atggtgaaac gcaagcgaat atcgatggtc tttacagcgg tcatgcgccc
     9421 cccccctgac cgcgcgcggt attgagcaag cgcaaaatct gcatacgctg ctacatggtg
     9481 tttcctttga tctggtttta tgcagtgaac tggaacgggc acagcatacc gcgcgactgg
     9541 ttctcagtga ccgccagctc cccgtgcaaa tcatccctga actcaacgaa atgttttttg
     9601 gcgactggga gatgcgacat catcgcgacc tcatgcaaga agatgccgaa aactatagcg
     9661 cgtggtgcaa tgactggcag catgcaatcc ccacgaacgg tgaaggattt caggcatttt
     9721 cgcaacgtgt ggaacgcttt atcgcaaggc ttagtgaatt tcagcactat cagaatattt
     9781 tagtcgtcag ccatcagggt gtactgagtc tgttaatcgc ccgtttaatt ggcatgcctg
     9841 ccgaggctat gtggcatttt cgcgttgacc aggggtgttg gagcgccatt gatatcaacc
     9901 aaaaattcgc gacgctacgc gtcctcaata gccgtgccat cggggtcgat aatgcatgac
     9961 ttttctgttt ttttacgggt aagccgcaac gaccattgac agacccgggc aggctgatat
    10021 tctccgcagc cagacttttt tcgccagaca cgactttgta gaaattgttt tacaaaaatg
    10081 gcgatgcaat ctgcggcgcg gggtgggatg atagcccact ttcgaaagcc gattcggcga
    10141 caattgtccc gaaatcgcct ctggttcagg tatactgaca gaccattttt atctatttga
    10201 ttcacccagg gggaaaactt gcagggtaaa gcactccagg attttgttat cgacaaaatt
    10261 gatgacctca aaggtcagga catcatcgcc ttagacgttc aaggcaaatc cagcatcacc
    10321 gactgcatga tcatctgtac gggtacgtcc agccgtcatg ttatgtccat tgctgaccac
    10381 gttgtgcagg agtctcgcgc agcgggcctg ttaccgctcg gcgtagaagg tgaaaacagc
    10441 gccgactgga ttgtcgtgga tttgggcgat gtgattgtcc atgtcatgca ggaagagagc
    10501 cgtcgcctgt atgaactgga aaaactctgg agttaatgcg tgaagctgca acttgtcgcc
    10561 gtgggaacga aaatgccgga ctgggtacaa accggtttta ccgagtacct gcgtcgtttt
    10621 ccgaaagata tgcccttcga gctgattgaa attccggccg gaaaacgcgg caagaatgcg
    10681 gacatcaagc gcatactcga caaagagggt gagcagatgt tggcggccgc aggcaaaaac
    10741 cgcattgtca ccctcgatat tccaggcaag ccctgggata cgccgcagtt agccgctgag
    10801 ctggaacgct ggaagctgga tggtcgcgac gtcagtctac tgattggcgg gcctgaaggg
    10861 ttgtcgcctg cctgtaaagc ggcggcggag cagagctggt cgctgtcggc gcttaccctc
    10921 ccccatccgc tggttcgcgt gctggtcgca gagagtctgt accgggcgtg gagcatcacc
    10981 accaaccatc cttatcaccg tgagtgataa gggagctttg agtagaaaac gcagcggatg
    11041 aaactacaga actcttttcg cgactatacg gcagagtccg cgctgtttgt gcgccgggcg
    11101 ctggtcgcct ttttggggat tttgctgctg accggcgtgc ttatcgccaa cctgtataat
    11161 ctgcaaattg ttcgctttac cgactaccag acccgctcta atgaaaaccg cattaagctg
    11221 gtgcctatcg cgcccagccg cggcattatt tacgaccgta atggtatccc tctggccctc
    11281 aaccgcacta tctaccagat agaaatgatg ccagagaaag tcgataacgt gcagcaaacg
    11341 ctggacgctt tgcgcagcgt ggttgatctg accgatgacg atattgctgc attccgaaaa
    11401 gagcgcgcac gttcacaccg tttcacctct attcccgtga aaactaacct gaccgaagta
    11461 caagtagctc gctttgccgt caatcagtac cgttttccgg gggttgaagt taaaggctat
    11521 aaacgtcgtt actatcctta cggttcggcg ttgacccacg tcatcggcta tgtgtcgaaa
    11581 atcaacgata aagacgtcga acgcctgaat aatgacggca aactggccaa ctatgcggca
    11641 acgcatgata tcggtaagct gggcattgag cgttactatg aagacgtgct gcacggtcag
    11701 accggttatg aagaggttga agttaacaac cgtgggcgtg ttatccgcca gttaaaagaa
    11761 gtaccaccgc aagccggaca cgatatttac ctgacgctgg atctcaaact ccagcaatat
    11821 attgaaacgc tgctggcggg tagccgcgca gctgtggtag tcaccgatcc gcgtacaggt
    11881 ggggtgctgg cgctggtttc cacgcctagt tatgacccaa acttgtttgt tgacggtatc
    11941 tccagcaaag attattccgc cttgttgaac gacccgaata caccgctggt gaaccgcgcc
    12001 acacaggggg tttatcctcc cgcgtctaca gttaaaccct atgtggcggt ttcggcattg
    12061 agcgccgggg tgatcacgcg caatacgacg ctgtttgacc caggctggtg gcaactgcca
    12121 ggttcggaaa aacgttatcg tgactggaaa aaatggggcc acgggcgtct gaatgtcaca
    12181 agatcgctgg aagaatctgc ggataccttc ttctatcagg tggcctacga tatggggatc
    12241 gatcgcctct ccgaatggat gggtaaattc ggttatggtc attacaccgg tatcgacctg
    12301 gcggaagaac gttccggcaa catgcctacc cgcgaatgga aacagaaacg ctttaaaaaa
    12361 ccgtggtatc agggtgacac cattccggtt ggtatcggtc agggttactg gacagcgacc
    12421 ccaatccaga tgagtaaggc actgatgatc ctgattaatg acggtatcgt gaaggttccg
    12481 catttgctga tgagcaccgc cgaagacggc aaacaggtgc catgggtaca accgcatgaa
    12541 ccgcccgtcg gcgatattca ttccggttac tgggagctgg cgaaagacgg tatgtacggt
    12601 gttgctaacc gccctaacgg tacgacgcat aaatactttg ccagcgcacc gtacaaaatt
    12661 gcggcgaaat ctggtaccgc tcaggtcttc ggtctgaaag cgaacgaaac ctataatgcg
    12721 cacaaaattg ccgagcgttt acgtgaccac aaactgatga ccgcctttgc gccatacaac
    12781 aatccgcaag tggctgtcgc catgattctg gagaacggcg gcgcgggtcc ggcggttggt
    12841 acgctgatgc gtcagatcct cgaccacatt atgctgggtg ataacaacac cgatctgcct
    12901 gcggaaaatc cagcggttgc cgcagcggag gaccattaat catgacggat aatccgaata
    12961 aaaaaacatt ctgggataaa gtccatctcg atcccacaat gctgctgatc ttactggcat
    13021 tgctggttta cagcgcccta gttatctgga gcgccagcgg tcaggatatt ggcatgatgg
    13081 agcgtaaaat cggccaaatc gcgatgggtc tggtcatcat ggtggtgatg gcgcaaattc
    13141 ctccacgcgt ttatgaaggc tgggccccct atctctatat catctgtatt attttgctgg
    13201 tggcggtaga tgctttcggt gccatctcta aaggtgctca acgctggctg gacctcggta
    13261 ttgttcgttt tcagccgtcg gaaattgcca aaatagccgt accactgatg gttgcgcgct
    13321 ttatcaaccg cgacgtttgc ccaccgtcgt tgaagaacac aggcatcgcg ctggtgctga
    13381 tatttatgcc cacgctgctg gtggctgcac agcctgacct gggaacatca atcctcgttg
    13441 cgctttccgg tctgtttgta ctgttcctct ctggccttag ctggcgtctg attggcgtcg
    13501 cagtagtgct ggtggcggcg ttcattccta ttctgtggtt cttcctgatg catgattacc
    13561 agcgccagcg cgtaatgatg ctcctagacc cggaatcaga cccactcggc gcgggctatc
    13621 acattattca gtctaaaatt gctattggct ccggcggatt acgcggcaaa ggctggctgc
    13681 atggcactca gtcacaactt gaatttctac ccgaacgcca taccgacttt atcttcgcgg
    13741 tactggcgga agagctggga ttagtgggca ttttgattct gctcgctctc tacattctgc
    13801 tgatcatgcg cgggctgtgg atagccgcca gagcgcaaac cacctttggt cgcgtcatgg
    13861 ctggcggctt aatgctgata ttattcgttt atgtcttcgt aaatattggt atggtaagcg
    13921 gtattctgcc ggttgtaggg gttccgctcc cactggtcag ttatggagga tcagcgctaa
    13981 ttgtgctgat ggctgggttc gggattgtaa tgtcaatcca cacccacagg aaaatgttgt
    14041 cgaaaagcgt gtaagaggtg cgcaatgcgt aagcagtggc tcgggatctg catcgcggca
    14101 ggaatgctcg cggcatgtac aagcgatgat ggtcagcaac agacggtaag tgtaccgcag
    14161 cctgcggtat gtaacggccc catagttgaa attagcgggg cggacccgcg tttcgaacca
    14221 ctgaacgcga cggcaaatca ggattaccag cgcgacggta aaagctacaa aatcgtgcag
    14281 gatccgtctc aatttagcca ggcgggactg gcggcaatct atgatgccga accaggcagt
    14341 aacctgacgg cctctggcga agctttcgat ccgacacagc tgacggcggc ccatccaacg
    14401 cttccgatcc ccagctacgc cagaatcact aacctggcta acgggcgaat gatcgtggtg
    14461 cgcattaatg atcgcggccc ttacggcaac gaccgcgtta tttcgctttc tcgcgcggca
    14521 gctgaccgtc ttaacacgtc aaacaacacc aaagttcgta tcgatccgat tattgtcgcc
    14581 caggatggtt cgctttctgg tcctggtatg gcttgtacca cagtcgccaa acagacttac
    14641 gccctgcctg cacctcccga tttaagcggt ggcgcgggaa caagttcagt gtctggcccg
    14701 cagggtgaca ttcttccggt cagtaattcg acgctaaaaa gcgaagatcc gaccggcgcg
    14761 ccggtaacca gtagcggttt cctcggcgca ccaacgacct tagcgcctgg tgtactggaa
    14821 ggcagcgaac cgacgcctgc gccacagccc gttgttacag ctccgtcgac aacgcctgca
    14881 acctcgcctg caatggtgac accgcaagcc gcctcgcaaa gcgccagcgg caactttatg
    14941 gtgcaagtcg gggccgttag cgatcaggct cgtgcgcaac agtaccaaca gcaactggga
    15001 cagaagttcg gcgtccccgg tcgcgtaact caaaatggcg cggtctggcg gatccagctt
    15061 ggccccttcg ccagcaaagc cgaagccagt accttgcagc aacgtttgca aaccgaagcc
    15121 caattacagt catttattac caccgcgcag tagcgtaaag caggcatctg atgtgtcaat
    15181 ttctgtaaat gacgttaaca aagtcacgcg gaaagtcaga tgcctgccgg tagtgcattt
    15241 gctatagtag ggcacttttt ttaattccat cacggatgtc gtagttcaga ccatgaatac
    15301 cattttttcc gctcgtatca tgaagcgcct ggcgctcacc acggctcttt gcacagcctt
    15361 tatctctgct gcacatgccg atgacctgaa tatcaaaact atgatcccgg gtgtaccgca
    15421 gatcgatgcg gagtcctaca tcctgattga ctataactcc ggcaaagtgc tcgccgaaca
    15481 gaacgcagat gtccgccgcg atcctgccag cctgaccaaa atgatgacca gttacgttat
    15541 cggccaagca atgaaagccg gtaaatttaa agaaactgat ttagtcacta tcggcaacga
    15601 cgcatgggcc accggtaacc cggtgtttaa aggttcttcg ctgatgttcc tcaaaccggg
    15661 catgcaggtt ccggtttctc agctgatccg cggtattaac ctgcaatcgg gtaacgatgc
    15721 ttgtgtcgcc atggctgatt ttgccgctgg tagccaggac gcttttgttg gcttgatgaa
    15781 cagctacgtt aacgccctgg gcctgaaaaa cacccacttc cagacggtac atggtctgga
    15841 tgctgatggt cagtacagct ccgcgcgcga tatggcgctg atcggccagg cgttgatccg
    15901 tgacgtaccg aatgaatact cgatctataa agaaaaagaa tttacgttta acggtattcg
    15961 ccagctgaac cgtaacggcc tgttatggga taacagcctg aatgtcgacg gcatcaaaac
    16021 cggacacact gacaaagcag gttacaacct tgttgcttct gcgactgaag gccagatgcg
    16081 cttgatctct gcggtgatgg gcggacgtac ttttaaaggc cgtgaagccg aaagtaaaaa
    16141 actgctgacc tggggcttcc gtttcttcga aaccgttaac ccactgaaag taggtaaaga
    16201 gttcgcctct gaaccggttt ggtttggtga ttctgatcgc gcttcgttag gggttgataa
    16261 agacatgtac ctgaccattc cgcgtggtcg catgaaagat ctgaaagcca gctatgtgct
    16321 gaacagcagt gaattgcatg cgccgctgca aaagaatcag gtcgtcggaa ctatcaactt
    16381 ccagcttgat ggcaaaacga tcgagcaacg cccgctggtt gtgttgcaag aaatcccgga
    16441 aggtaacttc ttcggcaaaa tcattgatta cattaaatta atgttccatc actggtttgg
    16501 ttaaaaatta aacacttgaa agtgtaattt ccgtccccat atactaagca tcagtaaaaa
    16561 aactcccgcc ttctggcggg agttgctatt taattacgtt acgccggagc tgacatgaaa
    16621 accaaactta acgaactgct tgaattccct actcctttta cttacaaagt tatggggcag
    16681 gcgttacctg agctggttga tcaggtggtt gaagtggtac agcgccatgc gccaggtgac
    16741 tacaccccaa cggtaaaacc aagcagcaaa ggcaactacc actcggtatc tatcactatc
    16801 aacgccactc atatcgagca ggttgaaata ctgtatgaag aactgggcaa aatcgatatt
    16861 gtccgcatgg ttctgtaact cgcttctccg ttacccggct cctggtcggg taactcccct
    16921 ctcctcgctg tgatatactt ttcccccact tttactcatt ctccacggag atgccgtttt
    16981 gtatcaggat aaaattcttg tccgccagct cggtcttcag ccttacgagc caatctccca
    17041 ggctatgcat gaattcaccg atacccgcga tgatagtacc cttgatgaaa tctggctggt
    17101 cgagcactat ccggtattca cccaaggtca ggcaggaaaa gcggagcaca ttttaatgcc
    17161 gggtgatatt ccggtgatcc agagcgatcg cggtgggcag gtgacttatc acgggccggg
    17221 acaacaggtg atgtatgtgt tgcttaacct gaaacgccgt aaactcggtg tgcgtgaact
    17281 ggtgaccttg cttgagcaaa cagtggtgaa taccctggct gaactgggta tagaagcgca
    17341 tcctcgggct gacgcgcctg gtgtctatgt cggggaaaag aaaatttgct cactgggttt
    17401 acgaattcga cgcggttgtt cattccacgg tctggcatta aacgtcaata tggatctttc
    17461 accattttta cgtattaatc cttgtgggta tgccggaatg gaaatggcta aaatatcaca
    17521 atggaaaccc gaagcgacga ctaataatat tgctccacgt ttactggaaa atattttagc
    17581 gctactaaac aatccggact tcgaatatat taccgcttaa ttccatacat caatggccca
    17641 atttacactg ggtcattact cataatacct gctaatgctt tacatctcct tcgcgtttgt
    17701 atattttctt tatcacttgc agaaatgcgg tgccacgcct tgctccgtaa ctcaccccat
    17761 tgattaacct attgaaatag ttgattatgg tgagcaaaag cgttccgttc caattagaca
    17821 taagattatt gtaatttttt aaatattatt tttccatgaa taaaattgga gtaagtgcgt
    17881 ggatagtaat aatcaaattg aaccctgttt aagcagaaag tcgtcagaag gtaaaccaca
    17941 aatatttaca accttacgaa atattgctct taaccttctg actatttttg aagctgtata
    18001 tgtacataaa gggatcgtta atgcagcgaa agtgcttaat ctgcccccct cggcaatcag
    18061 tcagtctatt cagaaactgc gcgttatatt ccctgaccca ttgtttattc gcaaaggcca
    18121 aggtgtcact cctaccgcat ttgcgatgca tctacatgag tagatcagtc agggccttga
    18181 gtccattctt ggcgcgctgg atatcgaagg aagctatgat aagcaacgaa cgataactat
    18241 tgcgaccact ccctcggtcg gagccctggt ccttcctgtc atctatcggg cgattaaaac
    18301 tcactatccg cagcttttac tgcgcaaccc gcccatcagc gacgcggaaa accaactcag
    18361 tcagtttcaa accgatctca tcatcgataa catgttttgc accaatcgta cggtgcaaca
    18421 tcatgttctg ttcaccgaca atatggtgtt aatttgccgc gagggaaatc cactactctc
    18481 tttagaagat gacagagaga ctatcgacaa cgctgcgcat gtacttctgt taccggaaga
    18541 gcaaaatttc agcggtctgc ggcagagagt tcaagaaatg tttccggacc ggcaaatcaa
    18601 tttcaccagc tacaacattt tgacaatcgc tgcactggtt gccaacagtg acatgttagc
    18661 gattattccc agccgttttt ataacctgtt tagccgctgc tggccgctgg aaaaactgcc
    18721 ttttccgtcc ttaaataagg agcaaataga tttctctatc cactacaaca aattcagcct
    18781 gcgtgatccc atcctgcacg gagtaataga tgttatccgt aatgcatttt gaaaaagtat
    18841 tgttacagcc agatcacagc gcagaacgat aaaatggcac aatccaacaa caattttaca
    18901 ttttcgcgac cgctttggct gctttcaggt ccgtttcaat gatatactgc cagtcgttaa
    18961 ttcaaaaata gttgataatt acaacaatct attgaattga aacgctttcc ttcgtaattc
    19021 gcaactggaa cacgcacgct atgagtaaac ccattgtgat ggaacgcggt gttaaatacc
    19081 gcgatgccga taagatggcc cttatcccgg ttaaaaacgt ggcaatagag cgcgaagccc
    19141 tgctgcgcaa gccggaatgg atgaaaatca agcttccagc ggactctaca cgtatccagg
    19201 gcatcaaagc cgcaatgcgc aaaaatggcc tgcattctgt ctgcgaggaa gcctcctgcc
    19261 ctaacctggc ggaatgcttc aaccacggca cagcaacgtt tatgatcctc ggcgctattt
    19321 gtacccgccg ttgtccgttc tgtgatgttg cccacggtcg cccggtagct cctgatgcca
    19381 atgaaccagt gaaactggcg cagaccattg ccgatatggc gctgcgttat gtggttatca
    19441 cctccgttga ccgtgatgac ctgcgcgatg gcggtgccca gcactttgcg gattgcatta
    19501 ctgccattcg ggaaaaaagc ccgcaaatca aaattgaaac tctggtgccg gatttccgtg
    19561 gtcgtatgga tcgtgctctg gatattctga ctgcaacgcc accagatgtg ttcaaccata
    19621 acctggaaaa cgtaccgcgt atttaccgtc aggtacggcc tggggcagat tacaactggt
    19681 cgctgaagct gctggaacgc tttaaagaag cgcatccgga aatcccgacc aagtctggtc
    19741 taatggtggg actgggtgaa accaatgaag aaattattga ggtaatgcgc gacctgcgcc
    19801 gtcatggtgt gacgatgtta acgctggggc aatatttgca gccaagccgc catcacctgc
    19861 cggttcaacg ttacgttagc ccggatgagt tcgacgaaat gaaagccgaa gcgctggcga
    19921 tgggctttac ccatgctgca tgcggtccgt ttgtccgttc ttcttaccac gccgatttgc
    19981 aggcgaaagg gatggaagtt aagtaatcct gtaataatta cttacatctg tctgataaaa
    20041 aacccgcttt ttgaagcggt ttttttgtat cgaacaagat attgagggag cgtcctgctc
    20101 gccacgtcac tctttatgag agagcttttc agcctgaaga tcaacgtctg cgcctttttt
    20161 cgccgcagca tcatcatcat tcatcgcctt cttgaaccct ttaatggccg ctccaaggtc
    20221 tccgcccagc gtacgtaact tcttagtccc aaacagcaga acgaccagcg ccgcaactac
    20281 tagcagtttg gtaatactaa tctcacccat agataccttc ttgacatata accggcaaca
    20341 gaacgcgcct taaaaaccag agaatattaa cggtcattcg acgcgcgcac acaatagcaa
    20401 tctgtaacaa ggtgaatcaa gcgccgagta aaaaaacatc ataataattg cggcggcgca
    20461 aagcgacggt tgtttaagac gggcagttgc gcgcgcacct gacgcacacg ttcgggcgtc
    20521 acttccgcca taatgagtgc aggcatttct gacgctgccg caatggtgac gccaaaggga
    20581 tctataatcc ggctttgccc gatatttttg ttcccgcact cgcccgccgc caccatataa
    20641 caggtggtat ccagcgcacg agcggcaagc aacgttgacc agtgatgctc tttgagcggc
    20701 ccgcgaaccc aggcggcagg aagtaccagg atttcagctc cctgtaatgc ctgtgccagc
    20761 gccagctctg gaaagcgtaa gtcataacag gtcatcagac cgaccttcat cccttccacc
    20821 tccagtaacg gagcgatttc attaccagca tcaacacggc gtgattcctg aatggcaaat
    20881 gcatcataga gatgcagttt ggcataacgg gcgacgatgt ttcctgcctg aagtgccacc
    20941 agcatattcc atgcgcgccc cggcgttgaa ggaacatgaa tcgtcagaat tgtcgtcatc
    21001 atgttacgtt tactttctcg ccgtaaacgt ccgaggaatt cgccttccag cagctgtgct
    21061 gatttaaccg atagatctgc atcatgatca tcgcgtgcca gcaatgcttc cggcaggaca
    21121 aacagcgata cgtcgttttc cgccgcctgc gccatcaacg aggcacaaat ctcagcgttc
    21181 ttttcccaca cagatgtaac agcaaactgt cctgcagcaa ccagcattgc cacctcctgt
    21241 cagagtcgta aaattcgcca acgacgatta ttcggcgtta cacttatcac tcatacaaat
    21301 caaatagcag gattttgcag tgttacaact tcttttagca gtttttattg gcggtggtac
    21361 gggaagcgtg gcgagatggc tgttaagtat gcgatttaac ccactgcatc aggcgattcc
    21421 gttggggacg ctgacagcaa acctgattgg ggcattcatc ataggaatgg gattcgcatg
    21481 gttcagcagg atgacgaaca ttgatccagt gtggaaagta ttaatcacca ccggattttg
    21541 tggcggtcta acaaccttct caacattttc ggcagaagtg gtgtttttgt tacaagaggg
    21601 tcgctttggc tgggcattac tgaacgtttt cgtcaacctt ctggggtctt ttgccatgac
    21661 agcactggct ttctggctgt tttcggcctc aaccgcacac taaagaccca aaaaaaaccc
    21721 gctgattaag cgggttttga attcttgctg acgtatctta cagagcgatt acgtttgcag
    21781 cagaagggcc tttggcaccg ttagtgattt cgaactctac gcgctgacct tcagcaagag
    21841 ttttaaaacc attagtctgg attgcagaga agtgtacgaa cacgtctttg ctgccgtctt
    21901 ccggagtaat gaaaccgaat cctttggact cattaaacca cttaacgtta cctttaatct
    21961 tagacatcaa aattaccttt acatgaaaaa tagacacaaa tgctgcgtcg gttaccagta
    22021 caccaattgt ggtacgcttt gtccagtgca actttactaa aaagtgataa aagtcgcgtt
    22081 atttttgatg ttacttattt tagctattga ttttaaagaa gttactaaaa cttcatttgt
    22141 ctcaaaactg aaagcgcatc caggcaaagt acacattgcc attgttgtag gtacccggaa
    22201 tgtaggtcat ctggaaagtc actgggccat aacccacgga ggccaatggc agtagaaccg
    22261 ggagagggat gtaattccag ttatcgcgtg ccgttacgcc agcggtgaat cccagaccta
    22321 aatgaaaatt ttcatccgcc agcggtcgcc aggtactttc ccatccgtat ccggcaatcg
    22381 gttcccattt gttccacgag tccttaaatg ccatggcata caggccatgc cagtttcctt
    22441 tttcatccca acgcgacagg ccaaaaccgc caccccacgg tcgctcgtta tagcgatcgg
    22501 ttttttcttt gtcgtaagcg aaacgtgcat gccaggtgat ggcaggaata tataaatcat
    22561 aatgttcagg ctgttgccag gttggtgcaa tattttctct aaacgttgtc atccactcat
    22621 ctgcgttagc aaaaacttta ccaacgctga ttaactgaat aaaaacaaag gaaaagatag
    22681 cgacatattt actcacgttc atttgtgacc ataaaacatt tatcaaaaat ctactactag
    22741 catagcaaag ctacaattaa cataacctta atagacccaa cataaagaat aatctgaata
    22801 gctgctctat ttatttccgc ttgttcaagt taaatatttc cgcgcatcga ttaaagatga
    22861 tgcgtcgttt tctgcgatgg gaatagtcaa aaaagaaaaa ccaagtcttt tttgatgaca
    22921 aaatgcaatc aaggaaaaat tattttattt tttaacgaga tacaacaatc accttaacaa
    22981 agtatataat atatatcgtc aacctatgct catattagat tattttttga gcgcacagcc
    23041 gcagctaaca ccattgcaat taacaaattt gcatcaatcc accatcaatt tgcacacatt
    23101 attatgtgat aattgccaac cgctaaatat gcgttttgtt atctatgtat aaaaacagta
    23161 acttcaatgt cttaatggca gtttttcttg attttaatca gcattcatcg ccaatttatt
    23221 gggcatattt tttccttaag ctttaggaat tttttattat ttactttggg gcctggagac
    23281 aggaaaaatt atgctgacat tcattgaact ccttattggg gttgtggtta ttgtgggtgt
    23341 agctcgctac atcattaaag ggtattccgc cactggtgtg ttatttgtcg gtggcctgtt
    23401 attgctgatt atcagtgcca ttatggggca caaagtgtta ccgtccagcc aggcttcaac
    23461 aggctacagc gccacggata tcgttgaata cgttaaaata ttactaatga gccgcggcgg
    23521 cgacctcggc atgatgatta tgatgctgtg tggatttgcc gcttacatga cccatatcgg
    23581 cgcgaatgat atggtggtca agctggcgtc aaaaccattg cagtatatta actcccctta
    23641 cctgctgatg attgccgcct attttgtcgc ctgtctgatg tctctggccg tctcttccgc
    23701 aaccggtctg ggtgttttgc tgatggcaac cctatttccg gtgatggtaa acgttggtat
    23761 cagtcgtggc gcagctgctg ccatttgtgc ctccccggcg gcgattattc tcgcaccgac
    23821 ttcaggggat gtggtgctgg cggcgcaagc ttccgaaatg tcgctgattg acttcgcctt
    23881 caaaacgacg ctgcctatct caattgctgc aattatcggc atggcgatcg cccacttctt
    23941 ctggcaacgt tatctggata aaaaagagca catctctcat gaaatgttag atgtcagtga
    24001 aatcaccacc actgctcctg cgttttatgc cattttgccg ttcacgccga tcatcggtgt
    24061 actgattttt gacggtaaat ggggtccgca attacacatc atcactattc tggtgatttg
    24121 tatgctgatt gcctccattc tggagttcct ccgcagcttt aatacccaga aagttttctc
    24181 tggtctggaa gtggcttatc gcgggatggc agatgcgttt gctaacgtgg tgatgctgct
    24241 ggttgccgct ggggtattcg ctcaggggct tagcaccatc ggctttattc aaagtctgat
    24301 ttctatcgct acctcgtttg gttcggcgag tatcatcctg atgctggtat tggtgattct
    24361 gacaatgctg gcggcagtca cgaccggttc aggcaatgcg ccgttttatg cgtttgttga
    24421 gatgatcccg aaactagcgc actcttccgg cattaacccg gcgtatttga ctatcccgat
    24481 gctgcaggcg tcaaaccttg gccgtaccct ttcgcccgtt tctggcgtag tcgttgcggt
    24541 tgccgggatg gcgaagatct cgccgtttga agtcgtaaaa cgcacctcgg taccggtgct
    24601 tgttggtttg gtgattgtta tcgttgctac agagctgatg gtgccaggaa cagcagcagc
    24661 ggtcacaggc aagtaaatag taatgccgga ggcaagtttc ctccggcttt atcatgtcac
    24721 ccactgtggt atatgcgttg tggtctgcca actttgccgt gaacaatttc agcaataatc
    24781 agatggcggc tggcgcaata ttcaagataa cgtctggcag acgtgcggct gatggtcagt
    24841 gcctgcgcca ccgtttctgc cgtatgttgc acaccaggct ctttaaacag ttttcgcacc
    24901 gcgtttagcg tcaagggatc aatgccggtc ggcagttcgt ctttaggttc accgcgagca
    24961 taggcattaa acatctcatc aatttgcttc tggctggcgc tatcaatact ttccagcata
    25021 tgtttacgct ggcggaaacg ggttagcgtt tgccccagcc gttcataggc aatgggctta
    25081 atgagataat caaatacacc acaacgtacg gcttcagaca ccgtttccat atcgctggct
    25141 gcagtggtaa acaccacgtc gccgggataa tgcgcctgca ccagttcatg caataaatta
    25201 atccctctgc cgtcaggaag atagttatcg agcaagatta gccccggctt aaaacgctcg
    25261 atcatcattc gggcctgcgc caggtttccc gccagtaata tctgactgaa tccgggaatg
    25321 tgacgaatat attccgcatg catctctgcc agcggcgttt cgtcctcaac gatcaatagg
    25381 gttaatggag ctgtcattag gtttcacttt cggaatatag attgaaaata aggtaccgca
    25441 gggatcatta tcttcgagag tgataacacc accgcagcgc gttacgtagc tggcaatcaa
    25501 gtacaaccca atgccatgtt caccgggctc gtcagcacgc gtactgaccc cctgctcaaa
    25561 tattttgtct cgtagagact ctggaacgcc gcagccctga tcggcgactt caatcaccac
    25621 atcatcgcct tcatcgctga ggaataattc aacgatcttg tttccttcat cgctacgcag
    25681 gctggcttcg aaggcgttat caagtaaatt gcccacaatg gctgcaaact cggtgctatc
    25741 cagtcctggc ggcagttgcg aaagctggct accggggaca atgatcattt ttagccccag
    25801 ttcccgggcg cgctgcactt taccaaaaag cagccccgcc acctagcgat cggcaaacgc
    25861 ctcgcgcagg ctgtcaataa gctgttgctg ggcctgagac tccccctgca ccatcgccag
    25921 cacgcgatca tactctttca tctgcaacag accattgagc gtcgacatcc aattgagatg
    25981 ctcgtgtcgc aatgtacgaa ggctctcaac gtattgtttt atttgcgtca gttgcgcatt
    26041 gagggtggaa atttcgtctt tactgcgaaa gctgataatg gcccccagca aatcatcacc
    26101 tgaacgaata gcttcccgat tggcaataac gctcagaccg ttaaagttag ccaccatatc
    26161 ctgacgtttt tcatcaatct gttcggtaaa gaaatcggcg ggcctgacca cttcagcaat
    26221 gggtttaccc aaccattgcc gtccggggga actcagcccc agcatctttc ttgcgttacg
    26281 attgatggcg gtaatgtaac cgtgcggatc caccgcaatc agcccttcat aaaccgaact
    26341 aaacagcgcc tcttgctgac ggaccacgcg tgcgatttgc tttggctcca tgcccatcat
    26401 ctgccgacgg atatgcgcgg ccaggaacca cgacagcaac atcagaatcc ctaacagcac
    26461 gacaaacaca cctgccatcg gtaataaaaa ctcagcccgc cagctatcga ttttactcac
    26521 caggtagcca atcgacacca cgccgatgac ttttccatcg tcatcaaaga ttggcgtttt
    26581 ggcgcgcatc gccatcccca ttgacccttt cccggtaatg aagtagctct cccctttctc
    26641 cagcgcgccc tgtttggtga actgcatagg ataaccaatt ttctccggat taggatggta
    26701 aaggcggatc gagtgccggt cgccatcacc acataatcaa aatcggtatc tctttgtaat
    26761 ttgttagcga tggtcgccag ccgtttgtag tcacgcgttt tcaccgcaga gatgacactg
    26821 tcattggagg caataatttt cgcctgattc attgccatgt cgcgtacatg aagcgttaaa
    26881 tagtcctcaa aactggccgt aaaatattgc gccaaggctg caatgacaaa tattgagaac
    26941 accagaatca gcaaaaagat acgcagcgga aatgccagtc tttggaaaaa tgcaaactgt
    27001 ttattctcgt taagctgcaa cattgttttc cttgcgggtt atcccgagcc tctttgacag
    27061 atgtaattaa aatgttgaat caaaatacat agatattaat tatgatcgtg gcaaatttta
    27121 ttcattaaat caattaaatc acttaaacct gcaggtcatt tcgtaattac attaattaaa
    27181 aaacttaaac cattaaataa ataaaaattt cacgccgaaa tactccttag gatgtatagc
    27241 gaaaggagaa aaagatatac ctcgatcacc cccctttctc ccaagtgaaa ataaaaggtt
    27301 atcagtttgc aacattgaac aacattcgtt gcaaatcgat aacaacatgc accttcagga
    27361 tactatttat tatgttcggc aatgatattt tcacccgcgt aaaacgttca gaaaataaaa
    27421 aaatggcgga aatcgcccaa ttcctgcatg aaaatgattt gagcgttgac accacagtcg
    27481 aagtatttat taccgtaacc cgcgatgaaa agcttatcgc gtgcggtgga attgccggaa
    27541 atattattaa atgcgttgct atcagtgaat ccgttcgcgg tgaaggactg gcgctgacat
    27601 tagccactga actgataaac ctcgcctatg agcggcacag cacgcatctg tttatttata
    27661 ccaaaaccga atacgaggcg ctgttccgcc agtgcggttt ttccacgctg accagcgtac
    27721 ccggcgtgat ggtgctgatg gaaaacagcg ccacgcgact gaaacgctat gccgaatcgc
    27781 tgaaaaaatt tcgtcatcca gggaacaaga ttggctgcat tgtgatgaac gccaatccct
    27841 ttacgaatgg tcaccgttat ctgattcaac aagctgcggc acagtgcgac tggttgcatc
    27901 tgtttttagt caaagaagat tcttcacgct tcccctatga agaccggctg gatctggtgt
    27961 taaaaggcac cgccgatatt ccacgcctga ctgtgcatcg cggctccgaa tacatcatct
    28021 cccgcgctac gttcccttgc tacttcatta aagaacagag cgtcattaac cattgttaca
    28081 ccgaaattga tctgaagatt ttccgtcagt acctcgctcc cgcactgggt gtaactcacc
    28141 gctttgtcgg tactgaaccc ttttgtcgcg ttaccgccca gtacaaccag gatatgcgct
    28201 actggctgaa aacgccgact atctccgcac cgcccatcga actggttgaa attgagcggc
    28261 tgcgttacca ggagatgccg atatccgctt cccgggtacg tcaactgctg gcgaaaaacg
    28321 atctcacggc tatcgcgccg ctggtccctg cagtcacgct gcattatttg cagaacctgc
    28381 ttgagcactc ccgccaggac gcggcagctc gtcaaaagac ccccgcatga gaaacaggtg
    28441 aaaaatgaaa ataaaccagc ccgccgttgc aggcaccctt gagtctgggg atgtgatgat
    28501 acgcatcgcc ccactcgata cgcaggatat cgacctgcaa atcaacagca gcgttgagaa
    28561 acagtttggc gatgcaattc gcaccaccat tctggacgtt ctcgcccgct acaacgtgcg
    28621 cggcgtacag ctgaatgtcg atgacaaagg cacactggac tgcattttac gtgcacgact
    28681 ggaagccctg ctggcacgcg ccagcggtat cccgactctg ccatgggagg attgccaatg
    28741 atttccgctt cgctgcaaca acgtaaaact cgcacccgcc gcagcatgtt gtttgtgcct
    28801 ggtgccaatg ccgcgatggt cagcaactcc ttcatctacc cggctgatgc cctgatgttt
    28861 gacctcgaag actccgtggc attacgtgaa aaagacaccg cccgccgcat ggtttatcac
    28921 gcgctgcaac atccgctgta tcgcgatatt gaaaccattg tgcgtgtcaa cgcgctggat
    28981 tccgaatggg gtgttaacga cctggaagcc gtcgttcgcg gtggtgcgga cgttgtgcgt
    29041 ctgccgaaaa ccgataccgc tcaggatgtt ctggatattg aaaaagagat cctgcgtatc
    29101 gaaaaagcct gtggtcgtga acccggcagc accggcctgc tggcggcgat tgaatctccg
    29161 ctgggcatta cccgcgcagt ggaaatcgct cacgcttccg agcgtttgat cggtatcgcc
    29221 ctcggtgcag aagactatgt gcgcaacctg cgtacagaac gctccccgga aggaactgaa
    29281 ctgctgttcg cacgctgttc cattttgcag gccgcgcgct ctgcgggtat tcaggcgttc
    29341 gataccgtct attccgacgc taacaacgaa gccggattcc tgcaagaagc cgcccacatc
    29401 aaacagctgg gctttgacgg caaatcgctg atcaacccgc gtcagattga tctgctgcac
    29461 aacctctacg caccgaccca gaaagaagtg gatcacgccc gccgcgtcgt agaagccgct
    29521 gaagccgccg ctcgcgaagg cctcggcgtg gtttccctga acggcaagat ggtggacggt
    29581 ccggttatcg atcgcgcccg tctggtgctc tcccgtgcag aactttccgg catccgcgaa
    29641 gaataaggca atcaaaatga cgcagaaaat tgaacaatct caacgacaag aacgggtagc
    29701 ggcctggaat cgtcgcgctg aatgcgatct tgccgctttc cagaactcac caaagcaaac
    29761 ctaccaggct gaaaaagcgc gcgatcgcaa actgtgtgcc aacctggaag aagcgattca
    29821 tcgctctggt ttacaggacg gcatgacggt ttccttccat cacgctttcc gtggcggtga
    29881 cctgaccgtc aatatggtga tggacgtcat cgcgaagatg ggctttaaaa acctgactct
    29941 ggcctccagc tccctgagtg attgccatgc gccgctggta gaacacattc gccagggcgt
    30001 ggttacccgc atttatacct ctggcctgcg cggtccactg gcggaagaaa tctcccgtgg
    30061 tctgctggcg gaaccggtgc aaatccactc tcacggcgga cgtgtgcatc tggtacagag
    30121 cggcgaactg aatatcgacg tggctttcct cggcgtccca tcctgtgatg aattcggtaa
    30181 tgccaacggt tacaccggta aagcctgctg tggctccctc ggctatgcaa tggttgatgc
    30241 cgacaacgca aaacaggtcg tgatgcttac cgaagaactg ctgccttatc cgcataatcc
    30301 ggcaagcatt gagcaagagc aggttgattt gatcgtcaaa gttgatcgcg ttggcgatgc
    30361 tgcaaaaatc ggcgctggtg cgacccgtat gaccactaac ccgcgcgaac tgcttattgc
    30421 ccgtagcgct gcggatgtga ttgtcaactc tggctacttc aaagaaggtt tctccatgca
    30481 aaccggcacc ggcggcgctt cgctggcggt aacccgtttc ctggaagaca aaatgcgtag
    30541 ccgcgatatt cgcgccgact tcgccctcgg cggtattact gcaacgatgg ttgatctgca
    30601 cgaaaaaggt ctgatccgca aactgctgga tgtgcagagc tttgacagcc atgctgcgca
    30661 atcgctggcc cgtaacccca atcacatcga aatcagcgcc aaccagtacg ctaactgggg
    30721 ttcgaaaggc gcatcggttg atcgtctcga cgtggtggta ctgagcgcgc tggaaattga
    30781 cacccagttc aacgttaacg tgctgaccgg ctctgacggc gtactgcgtg gtgcttccgg
    30841 tggtcactgc gataccgcga ttgcctctgc gctttccatc atcgtcgcgc cgctggtacg
    30901 cggtcgtatt ccgactctgg tggataacgt gctgacctgc atcaccccgg gctccagtgt
    30961 cgatattctg gtcacagacc acggtatcgc agttaacccg gcacgtccgg agctggcaga
    31021 acgtctgcag gaagcgggca ttaaagtggt ttccattgag tggctgcgtg aacgtgcgcg
    31081 tctgctgacc ggtgaaccac agccgattga attcaccgac cgcgtcgttg ccgttgtgcg
    31141 ttaccgcgat ggctcggtga tcgatgttgt acatcaggtg aaggaataag ccatgcacct
    31201 gcttcctgaa ctcgccagcc accatgcggt gtcaattccc gagctgctcg tcagccggga
    31261 tgaaaggcaa gcacggcaac acgtctggct caagcgccat cctgttccac tggtctcctt
    31321 taccgtggtt gcgcctaggc cgattaaaga cagtgaggtc acacgccgaa tttttaatca
    31381 tggcgtgaca gccttgcgtg ccttagccgc aaaacagggc tggcaaattc aggagcaggc
    31441 tgcactggtt tccgccagcg ggccggaggg tatgttgagc attgccgccc cggctcgcga
    31501 cctcaagctc gccaccattg agcttgaaca tagtcatcct ctcgggcggt tatgggatat
    31561 cgatgtcctg acgcccgaag gcgaaattct ctcccgccgc gactattcac tgccgcctcg
    31621 ccgctgcctg ttgtgcgagc aaagcgcagc cgtctgcgcg cgtggaaaac ccatcaactg
    31681 accgatttac tcaaccgcat ggaggcactg ctgaacgatg tcgatgcctg caacgtcaac
    31741 taaaaccaca aagcttgcga cgtcattaat cgatgagtac gccctgctgg gctggcgcgc
    31801 catgctgact gaagtcaatc tgtcaccgaa accaggtctc gtggatcgca ttaactgcgg
    31861 tgcgcataaa gatatggcgc tggaagattt ccaccgcagc gcgctggcga ttcagggctg
    31921 gctaccccgt ttcattgaat ttggtgcctg tagtgcggaa atggcaccag aagcggtact
    31981 ccacggatta cgcccaattg gtatggcttg caaaggtgat atgttccgcg ccaccgcggg
    32041 cgtaaacacg cataagggca gcattttttc tttagggcta ctgtgtgcgg caattggccg
    32101 tttgcttcag ctcaaccagt cgctaacgcc aacaaccgtt tgttctacgg cggcaagttt
    32161 ctgccgtggc ctgaccgatc gcgaactgcg taccaataat tcacaactga cggcaggtca
    32221 acggttgtac cagcagcttg cccttaccgg cgcacgcggt gaagccgaag cgggttatcc
    32281 actggtgatc aaccacgcct tgccgcatta cctcactctg ctggatcagg ggttagatcc
    32341 tgaactggca ttgctcgata ccttgctcct actgatggcg atcaacggcg ataccaacgt
    32401 tgcttcgcgc ggtggcgagg gggcctgcgc tggctacagc gcgaggcgca aacattattg
    32461 caaaaagggg gcattcgaac ccccgccgat ctcgattatc tccggcagtt cgacagggag
    32521 tgtatcgaac gaaatctcag tccaggcggc agtgccgacc tgctgatcct tacctggttt
    32581 ttagcacaga tttaattatt taagcacttg ataaatttgg aaatattaat tttcggagaa
    32641 cccgtatgtc tttagcaaaa gataatatat ggaaactatt ggccccactg gtggtgatgg
    32701 gtgtcatgtt tcttatccct gtccccaacg gtatgccacc acaggcatgg cattacttcg
    32761 ctgtgtttgt ggcaatgatt gtcggcatga tcctcgagcc aattccggca acagcgatca
    32821 gttttattgc ggttactatt tgcgttattg gcagtaatta cctgctcttt gatgccaaag
    32881 aattagctga cccagcgttt aatgcgcaaa aacaggcgct gaaatggggt ctggctggtt
    32941 tttccagcac cacggtatgg ctggtatttg gcgcatttat ttttgcatta gggtatgaag
    33001 tttccgggtt aggtcgtcgc attgcccttt tcctggtgaa attcatgggc aaacgcacgc
    33061 tgacgttggg ttatgcgatt gtcattatcg acattctgct ggcgccgttt acaccgtcca
    33121 acaccgcgcg taccgggggt acggtttttc cggtcattaa aaacctgccg ccgttgttta
    33181 aatcattccc caacgatccg tccgcgcgtc gtattggcgg ctatttgatg tggatgatgg
    33241 tcattagtac cagtctgagt tcgtccatgt ttgtcaccgg tgcggcacca aacgtgctgg
    33301 gtctggagtt cgtcagcaaa attgccggta tccagattag ctggttgcag tggttcctct
    33361 gcttcctgcc ggttggggtt atcttgctta tcattgcgcc gtggctttcc tacgtgctgt
    33421 acaaaccgga aatcacacac agtgaagaag tggcaacctg ggcgggtgat gaactgaaaa
    33481 ccatgggtgc gctgacacgc agagagtgga cactgattgg ccttgtattg ctcagcttag
    33541 gtttgtgggt ttttggcagt gaagtcatta atgctactgc agttggtctg ctggcagttt
    33601 cgctaatgct ggccctgcac gttgtgcctt ggaaagatat tacccgctat aacagcgcat
    33661 ggaacacgct ggtcaacctg gcaactctgg ttgtgatggc taacggcctg actcgttctg
    33721 gctttattga ctggttcgcc ggtaccatga gtacgcacct ggaaggattc tcaccaaacg
    33781 caacggtgat tgtactggtt ctggtgttct actttgcaca ctacctgttt gccagcctgt
    33841 ctgcgcacac cgcaaccatg ctgccggtta ttctggccgt cggtaaaggt attccgggcg
    33901 taccaatgga acaactgtgt atcctgctgg tgctgtctat cggtatcatg ggctgtctga
    33961 cgccctatgc aaccggtcct ggggtgatta tttacggctg tggctatgtg aaatcaaaag
    34021 attactggcg tcttggcgca atcttcgggg tgatttatat ctctatgttg ctgttggttg
    34081 gctggccgat tctcgccatg tggaactaat acgttcgcca aacgctgaat aaatcatcac
    34141 gcccgccagg taagcccacc tggcgggcgt gatgatttaa tagatagtat tcagtgtatg
    34201 tatgagttcc acacccatta tgaaagcatt ctggcgtaac gccgcgttgc tcgcggtttc
    34261 tctgcttccc ttctcttctg ccaacgccgt agcgttgcag gcaaaacagt atggcgattt
    34321 tgatcgctat gtcctggccc tctcctggca aaccggattt tgccagagtc aacacgatcg
    34381 aaatcgtaac gaacgagatg aatgtcgcct gcaaaccgaa acgaccaaca aagctgattt
    34441 tctgaccgta catggtctgt ggccaggatt gcctaaatcg gttgctgccc gtggtgttga
    34501 tgaacgccgc tggatgcgct tcggttgcgc tactcgccca atcccgaatc taccagaagc
    34561 gcacgccagc cggatgtgtt catcgccgga aaccgggtta tcactggaaa cggccgctaa
    34621 actaagtgaa gtcatgccag gagctggcgg acgttcctgc ctggaacgct acgaatatgc
    34681 caaacacggt gcctgctttg gttttgatcc ggacgcatac ttcggtacga tggtacgcct
    34741 gaatcaagaa attaaagaga gcgaagccgg aaaattcctt gcggataatt acggtaaaac
    34801 agtgagccgc cgtgactttg acgccgcctt tgccaaaagc tggggaaaag agaacgtgaa
    34861 agcagttaag ctaacgtgcc agggtaaccc tgcgtatttg actgaaattc aaatctcgat
    34921 caaagctgac gccatcaacg ctccgctttc tgcaaactca ttcttgccac aaccacaccc
    34981 aagcaactgt ggcaaaacct ttgtgattga taaagtgggt tattaatcat ctgatttaca
    35041 aaacacgcta attcgtcttc cataaaccgt gacgattaag aattatacat aatgcgctgc
    35101 attgaggaat tccctgaatg tgacgcaaat cacttcaaag agtgagcaac agtcagtatc
    35161 atcatgtgag ttaaaccctc gccgcctgac ggtgagggtt ttcttttggg tatgtttatc
    35221 tgcgacactc gcagtaccga cgacatggag taaaaatgtc cagaccaact atcatcatta
    35281 acgacctgga tgccgaacgc atcgatattc tgctggagca acccgcctat gctggtttgc
    35341 caatcgccga cgcgttaaac gcagagttgg atcgcgccca aatgtgttcg ccagaagaga
    35401 tgccacacga cgtggtgaca atgaacagcc gggttaaatt ccgcaatctt agcgatggcg
    35461 aagtgcgtgt gcgcacgctg gtgtatccgg caaaaatgac cgatagcaat actcagcttt
    35521 ccgttatggc tccggtaggt gccgcactgc tggggctgcg cgttggcgat tccattcact
    35581 gggaacttcc gggcggcgtt gcaacccacc ttgaagtgct ggaactcgaa taccagccag
    35641 aagctgctgg cgactacctg ctttaatccc atctgcacaa gcgataactc gctttgccca
    35701 gaggatgcac tcgcatcctc tttacgcaaa atcagcaatt tttcctggtt ctcactgttc
    35761 acttttgctg caaaattatt ccgtttcgtt tgtgggaata accatggcag aagcatttta
    35821 catcctgata ggatttctga ttatggcggc gattatcgtc atggctgtcc tttacctcga
    35881 aaaccactcc tgacgatcat attgttcccc ccggcatcgc attcaccaga cctgaaactg
    35941 ccttctgcgc ctcctctgcg ctttgtgcac cgggtaccag aatcacctta cggcactcct
    36001 cttcacgttt ttcgaaaatc tcatatcccc gggcggcctc ttcaaacggc atatagtggg
    36061 taacaatttc ttctggtttc agtaatcctt tctcaattaa cggtaataat tctcccagcc
    36121 atgcgtgaac gtgggtctgt cccattttaa acgtcaaccc tttatcaaag gcgtcaccaa
    36181 acaggaaacc gtgaataaat ccagcgtaga cgcccggtac gctaacaatg ccgccacgcc
    36241 tgaccgccgc aatacactgg cgcaacgctt taccgctgct gccctccagt ttcaggttag
    36301 tcagcaccgt ttccgtggtg ctgcctttcg cttcaaaacc gacggcgtct attactgcat
    36361 ccacgccccg gtgacctgcc gtttgttcaa taattgactg tgccggatcg ctatcttcat
    36421 cgaaattaat cgggatcgcg ccgtagcggt cagcggcgaa actcaagcgg tagggatgat
    36481 gatcaacaac aaaaatctgt tccgcaccga gcaaccgtgc acaggcgatt gtcaacaatc
    36541 ccactggacc ggcaccatag actgcaacgc ttgaaccttg ttggatctgc gcattttttg
    36601 ctgcctgcca tgccgttggc agaatatcag aaaggaaaag ggctttatca tctgaaagca
    36661 aaggcggtac tttaaacggc cccacattcc ctttagggac gcggacatat tccgcctgtc
    36721 caccaggaac gccaccatac aggtgactat aaccaaacaa tgccgcggga gctggtatct
    36781 gttttttatt gagcgcagcg cctttacccg cattggtatt ttcgcaggcg gcatattgct
    36841 gcattcgaca gaaaaaacag tcgccacaag caatgacaaa cggaattacc acccggtcgc
    36901 ctttttgcaa attttttacg tcctttccgg tttcaacgac ttcccccata aattcatgac
    36961 caaaaatatc gccatgttta acctagggta ttttgcctcg ataaagatgg agatcagagc
    37021 cacagatcgc cgttgccgta atacgcagaa taatatcatc tgcctgttca atgcccggat
    37081 cgggaacatt ttctacctga acgtgatgtg ggccgtgata agtcaatgct ttcatgcaac
    37141 ctccagagac agttcaaaaa agagtcagtc tttaagggta gctatcacag cgggaaaacg
    37201 ggcggtaccg caggttttgg gagaattcct ggtgttcagt gagaatatat taatcacaga
    37261 aaccgttcgt aaggaaaaag aaccagacaa atatcaggga taaatattat aatcatggcg
    37321 aatgatttgt tcatgattaa cagggagaaa gggttatgta taagacaatc attatgccag
    37381 ttgatgtatt tgaaatggaa ttgagcgaca aagctgttcg ccacgctgaa ttcctcaccc
    37441 aggatgacgg agttattcat ctacttcacg tactacccgg gtcagccagc ctgagcctgg
    37501 accgttttgc cgctgatgtg cgtcgttttg aagagcatct gcaacatgaa gcagaagaac
    37561 gtctgcaaac gatggtcagc cacttcacca tcgatccttc ccgcattaaa caacatgtcc
    37621 gttttggtag cgtgcgggat gaagtcaatg agttggcaga agaactgggg gctgatgttg
    37681 tagttatcgg ttctcgcaac ccatcgattt cgacccatct gttaggttct aacgcctcga
    37741 gcgtgatccg ccacgccaat ctgcctgtac tggtcgttcg ctaacagata aaagtattta
    37801 tgccgccctg cctggaacat cccggcaggg ctttttaatg ccataaaaaa gccgctaggt
    37861 ttgaccccgg cggctaagca attgcaggtg aatcttactt cttcttatgc agttttggtg
    37921 cgaatcaggt agtcaaaagc actcagagag gctttggcac cttcgccagt ggcgatgatg
    37981 atctgcttgt acggaaccgt cgtacagtca cccgctgcga atacgccttt gacgttggtt
    38041 tcgcatttcg catcaatgat aatctcaccc atgcggttac gttcgactgc gccttcgagc
    38101 cagttggtgt tcggcagcag accaatctgc acgaaaatac cggccagttc gatgttgtga
    38161 atatcgccgc tgacacgatc tcgatattcc agaccaacga ctttgctgcc gtcgcctttc
    38221 acttccgtgg tttgcgcatt cagaataatg tcgacgtttt tcaggctgcg cagtttgtcc
    38281 tgcagaacct ggtcggcttt catttctggc gcaaattcca gcagcgttac gtgctcaacg
    38341 atacccgcca ggtcaattgc cgcttccacg ccggagttac cgccgccgat aaccgctacg
    38401 cgtttacctt taaacagcgg gccgtcgcag tgcgggcagt aggtcacgcc tttggtgcga
    38461 tactgatctt cgcccggaac gttcatgttg cgccattttg caccggtcgc cacgataatg
    38521 ctgcgtgctt tcagtaccgc gccagaagct gtttcaatct gatgcagacc accttcaact
    38581 gctgctggga tcagtttgct ggcgctctgg ctgtcgatca catcaacgtc gtattcatca
    38641 acgtgaactt tcagtgcgcc tgccagcttc tgcccttcag tcttcggtac agaaatgtag
    38701 ttttcgatat caacggtatc gaggatctga ccaccaaaac gttcgcccat cagaccggta
    38761 cggatgcctt tacgtgcgga gtaaattgct gccgctgcac ccgccgggcc ggaaccgacg
    38821 attaatacgt cataagcatc acgcttgttc agctcttctg ccgcacgttt ttccgcgccc
    38881 gtatcaattt tggcaacgat ttcagtcaac gtcatgcggc cctgaccaaa ctctttcccg
    38941 tttacgaaca ctgccggaac gcccatcacg ttgcgatcgg tgatttcgtt ctggaaggtg
    39001 ccgccgtcaa ttgcagtgtg cttgatgcgc gggttcagta cgctcatcag gttcagcgcc
    39061 tgcaccacgt ccgggcagtt atggcaagag agcgagtaat aggtttcgaa ttcaaaatca
    39121 ccgtcaatat ggcgaatctg ctccagcaga gactgcgctt ctttcgacgg atgaccaccg
    39181 gtccacagca acgccagtac cagcgaggtg aactcgtggc ccagcgggga gcctgcaaaa
    39241 cgtggcccct ggttggaacc tgggttggtg atcaggaaag acggcttacg caccggcaag
    39301 ctgttatctt ctttaaaggt gactttgtct gacagttctg cgatttcagc caacagttcc
    39361 ttgatttctg ccgatttagc gctgtcatcc agcgtggcaa ttaactcaac aggcttggtc
    39421 aatttctcaa ggtaagcctt gagttgagtt ttcatatttg tgtcgagcat gtttatctcc
    39481 tgggctttaa acatcatcat gcaagcggcc tctacggcta cctggatgca gcttgcatca
    39541 tagtgcgggg aagaaaatcg ggtgccgcag cacccgaaga attaggtgaa tttccgaagt
    39601 atttcgcgcc ggagttggta gcgcgaaaga cgaaggaaat ttagatttta ccaaccaggt
    39661 ccagagacgg agccagagtt gcttcacctt ctttccattt agccgggcaa acttcacctg
    39721 ggtgagaagc tacgtactgt gctgctttga ttttacgcag caggtcagac gcgtcacggc
    39781 caatgccttc agcggtaact tcgattgcct ggatgatacc ctgcgggtca acaacgaagg
    39841 tcgcacggtc agccagacct tcatcttcac gcatgttgtc gaagttacgg gtcagggcgc
    39901 cagtcgggtc gccgatcatc gcatatttga ttttagcgat ggtttcagag ctgctgtgcc
    39961 atgctttgtg ggtgaagtga gtatcggtag atactgcgta tacgtctacg cccagtttct
    40021 gcagttcttc gtagtggtca gcaacgtcac ccagttcggt cgggcatacg aaagtaaagt
    40081 cagccgggta gaagaagaag acgctccagc ggccttcggt atctttttcg gtgatttcga
    40141 tgaattcgcc gtttttgaat gcctggtttt taaaaggttt aattttggtg ttaatcaagg
    40201 acatctatac ttcctccgtg ttttcgatga gatgtaaggt aaccaatttt tgccgattcg
    40261 gctaatgcgt ttccattatc aaatcgataa gttttacctt acaaccttcg taagacaact
    40321 ttgtgaactt tgcaccgcca gcggcgataa aaagtaaaat ggcccccttt ccaggtgacc
    40381 gtgtaatctg aactaccctt tggtatcttc agcgcctgcc tgcaggctga ggcgttgccc
    40441 ttacaaatgc aacaacgaca tggattacaa caccctcata aacaaagggc aatcacctga
    40501 tctaagctct tacctatgac agtgataggt tatgcctttt actcgacttt tgcactgact
    40561 gaaaaggaca aattaatgtt aaaaaagata cttttactgg ctctgcttcc tgcaatcgcc
    40621 ttcgcagagg aacttcctgc tccagtaaaa gcgattgaaa aacagggcat tacaatcatc
    40681 aaaacgttcg atgcccccgg aggaatgaaa ggttatctcg gaaagtatca ggatatgggc
    40741 gtcaccatct acctgactcc agatggtaag cacgctatct ctggttacat gtacaacgag
    40801 aaaggtgaaa acctgagtaa cacacttatc gaaaaagaaa tttacgcacc agccggacgc
    40861 gaaatgtggc aacggatgga acaatcccac tggctcctcg acggtaaaaa agatgcgccg
    40921 gtcattgtct acgtcttcgc cgatccgttc tgcccatatt gtaaacagtt ctggcagcag
    40981 gc
//
LOCUS       DADLFA010000010        40544 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000010 DADLFA010000000
VERSION     DADLFA010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40544
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.010"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(184..1041)
                     /gene="murI"
                     /locus_tag="JQH65_000485"
     CDS             complement(184..1041)
                     /gene="murI"
                     /locus_tag="JQH65_000485"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAY6137996.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
     gene            complement(986..2834)
                     /gene="btuB"
                     /locus_tag="JQH65_000486"
                     /pseudo
     CDS             complement(986..2834)
                     /gene="btuB"
                     /locus_tag="JQH65_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
     regulatory      complement(2888..3078)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3192..4292
                     /gene="trmA"
                     /locus_tag="JQH65_000487"
     CDS             3192..4292
                     /gene="trmA"
                     /locus_tag="JQH65_000487"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HAY6137997.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPCILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     gene            complement(4332..4691)
                     /locus_tag="JQH65_000488"
     CDS             complement(4332..4691)
                     /locus_tag="JQH65_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HAY6137998.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4691..5341)
                     /gene="fabR"
                     /locus_tag="JQH65_000489"
     CDS             complement(4691..5341)
                     /gene="fabR"
                     /locus_tag="JQH65_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HAY6137999.1"
                     /translation="MMGIRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            5662..7062
                     /gene="sthA"
                     /locus_tag="JQH65_000490"
     CDS             5662..7062
                     /gene="sthA"
                     /locus_tag="JQH65_000490"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HAY6138000.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLVLDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            complement(7045..7962)
                     /gene="oxyR"
                     /locus_tag="JQH65_000491"
     CDS             complement(7045..7962)
                     /gene="oxyR"
                     /locus_tag="JQH65_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HAY6138001.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(8229..9602)
                     /gene="argH"
                     /locus_tag="JQH65_000492"
     CDS             complement(8229..9602)
                     /gene="argH"
                     /locus_tag="JQH65_000492"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HAY6138002.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDAFDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSHVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            complement(9663..10439)
                     /gene="argB"
                     /locus_tag="JQH65_000493"
     CDS             complement(9663..10439)
                     /gene="argB"
                     /locus_tag="JQH65_000493"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HAY6138003.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            complement(10447..11451)
                     /gene="argC"
                     /locus_tag="JQH65_000494"
     CDS             complement(10447..11451)
                     /gene="argC"
                     /locus_tag="JQH65_000494"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HAY6138004.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPAINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            11605..12756
                     /gene="argE"
                     /locus_tag="JQH65_000495"
     CDS             11605..12756
                     /gene="argE"
                     /locus_tag="JQH65_000495"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HAY6138005.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDIDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVIERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            13108..15759
                     /gene="ppc"
                     /locus_tag="JQH65_000496"
     CDS             13108..15759
                     /gene="ppc"
                     /locus_tag="JQH65_000496"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HAY6138006.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEAPPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYKSWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIR
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDVYRGYVRENKDFVPYL
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            15942..17675
                     /gene="cptA"
                     /locus_tag="JQH65_000497"
     CDS             15942..17675
                     /gene="cptA"
                     /locus_tag="JQH65_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HAY6138007.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            17890..18741
                     /locus_tag="JQH65_000498"
     CDS             17890..18741
                     /locus_tag="JQH65_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY6138008.1"
                     /translation="MYHDISYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPTTTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(18728..19069)
                     /locus_tag="JQH65_000499"
     CDS             complement(18728..19069)
                     /locus_tag="JQH65_000499"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HAY6138009.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(19071..19949)
                     /locus_tag="JQH65_000500"
     CDS             complement(19071..19949)
                     /locus_tag="JQH65_000500"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HAY6138010.1"
                     /translation="MTSSAGQRISCNVVETRLNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLNIRQIHLLPFHQYGEPKYRLLGKTWSMKDVAAPSSADVAT
                     MREMAERAGFQVTVGG"
     gene            complement(19915..22212)
                     /locus_tag="JQH65_000501"
     CDS             complement(19915..22212)
                     /locus_tag="JQH65_000501"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAY6138011.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAVLAETMAANCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDEQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLWEAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(22263..22583)
                     /locus_tag="JQH65_000502"
     CDS             complement(22263..22583)
                     /locus_tag="JQH65_000502"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HAY6138012.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEARESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(22598..23677)
                     /locus_tag="JQH65_000503"
     CDS             complement(22598..23677)
                     /locus_tag="JQH65_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HAY6138013.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVVI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            23986..26487
                     /gene="ptsP"
                     /locus_tag="JQH65_000504"
     CDS             23986..26487
                     /gene="ptsP"
                     /locus_tag="JQH65_000504"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAY6138014.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLRQHLLTGVSEGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMTQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            26499..27161
                     /gene="fsa"
                     /locus_tag="JQH65_000505"
     CDS             26499..27161
                     /gene="fsa"
                     /locus_tag="JQH65_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAY6138015.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            27172..28275
                     /gene="gldA"
                     /locus_tag="JQH65_000506"
     CDS             27172..28275
                     /gene="gldA"
                     /locus_tag="JQH65_000506"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HAY6138016.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAAFSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYATLLVADQYGQRFLQEWE"
     gene            28549..29166
                     /locus_tag="JQH65_000507"
     CDS             28549..29166
                     /locus_tag="JQH65_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HAY6138017.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGNTLFYDPAYVRLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWKLKRPDSNIDHRRVPNLETWFSRHNKTRPISKNAGDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(29193..30131)
                     /gene="yijE"
                     /locus_tag="JQH65_000508"
     CDS             complement(29193..30131)
                     /gene="yijE"
                     /locus_tag="JQH65_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HAY6138018.1"
                     /translation="MSTGKLHTGSLMSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVT
                     SYIGAFDFTALRCIFGALVLFIVLLLRGRGMHPTPFKYTLAIALLQTCGMVGLAQWAL
                     VSGGAGKVAILSYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFS
                     SMKSAMLAILSGVSWGASAIVAKRLYTRHPRVDLLSLTSWQMLYAALVMSVVALLVPQ
                     REIDWQPTVFWALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWL
                     LGENPGAVEGSGIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(30192..32372)
                     /gene="katG"
                     /locus_tag="JQH65_000509"
     CDS             complement(30192..32372)
                     /gene="katG"
                     /locus_tag="JQH65_000509"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HAY6138019.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     SSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(32701..33591)
                     /gene="metF"
                     /locus_tag="JQH65_000510"
     CDS             complement(32701..33591)
                     /gene="metF"
                     /locus_tag="JQH65_000510"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HAY6138020.1"
                     /translation="MSFFHASQRDALNQRLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(33940..36372)
                     /locus_tag="JQH65_000511"
     CDS             complement(33940..36372)
                     /locus_tag="JQH65_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HAY6138021.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(36375..37535)
                     /gene="metB"
                     /locus_tag="JQH65_000512"
     CDS             complement(36375..37535)
                     /gene="metB"
                     /locus_tag="JQH65_000512"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAY6138022.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWTNNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            37812..38129
                     /gene="metJ"
                     /locus_tag="JQH65_000513"
     CDS             37812..38129
                     /gene="metJ"
                     /locus_tag="JQH65_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HAY6138023.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            38313..38921
                     /locus_tag="JQH65_000514"
     CDS             38313..38921
                     /locus_tag="JQH65_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HAY6138024.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTVKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            39066..39741
                     /locus_tag="JQH65_000515"
                     /pseudo
     CDS             39066..39741
                     /locus_tag="JQH65_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(39913..40185)
                     /locus_tag="JQH65_000516"
     CDS             complement(39913..40185)
                     /locus_tag="JQH65_000516"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6138025.1"
                     /translation="MELQLSDKGVIKMKAIQCCYFLALVSSNLMAGVMGNDNIFLSNS
                     DSAALYCVAKIFPDGENILIYKVNIINASSKKDVFFMKAQHQPTMT"
     gene            complement(40151..40544)
                     /locus_tag="JQH65_000517"
                     /pseudo
     CDS             complement(40151..40544)
                     /locus_tag="JQH65_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
BASE COUNT         9970 a        11143 c        10081 g         9350 t
ORIGIN      
        1 tttcccgcta cagagtcaag catttatttt tgctttctct gtcggaattc tcaggagaac
       61 cccgctgacc cggcggcgtg tttgccgttg ttccgtgtca gtggtggcgc attataggga
      121 gttattccgg cctgacaaga ggaaatttaa aataattttc tgaccgcgca acattcaacc
      181 aaatcagcct aaaactgcca gtttttcgag cgtttcgaag ccgtaacgct gtaaaacggg
      241 caataattgt tcagcttctg gcgtcattgc catacaaaag gcaatattcg catcggcaga
      301 ttttgcatcc ggggcttcat gttctaacaa ccaggccgtt cggcgagcaa tcgctgcgcc
      361 agaatccact agccgggttc cctctggcag cacttgtaac agttcttctt gtagtagagg
      421 gaaatgggtg caacccaata caacggtatc tggcggctct ttcattctta accacgggcg
      481 taggatacgt tttagtgcat ccagagaaac atcttcgcca tgtagcttcg cttcagccaa
      541 ctcaaccatc tctgccgagc ccagcatttc tatctggcat tcattagcga aacgcgcgat
      601 cagctcatga gtataagaac gtttaactgt tccgcgggtt gccagtaatc cgacaatgcc
      661 atttgccgtc agacgtgcag caggtttaat cgccggcacg acaccaacaa ccgggaagtc
      721 gaacttttcg cgtaatgcag gaagtgaaac ggtactggca gtgttgcaag cgaccacagc
      781 cagcgcaagg ggataacgtt cttgcaccgc ggtgacaatt gccaccactc gctcaacaat
      841 aaacgcttcg cttttttcgc catacgggaa agcgacgtta tcgaaagcat aaatgtaatg
      901 gagatccggt aagagatgcc ggatctcgtc atagaccgac aacccaccga cgccggagtc
      961 aaacaccagc acggtgggac gtggttcaga aggtgtagct gccagacaag gtgtattccc
     1021 gtcctgcagt ttggtagcca tagactgtct cataatcttt gtcgaacagg ttggctattt
     1081 taccacgaac tgtcaggtga gaggtgaccg gatacgcaac cgcaagatcc cacaagctca
     1141 caccgcccat tttaacggtt tgataaggat aagatgagta atccttatca tagcgagtgc
     1201 ctaaatactg ataagtaata ccccagtcga agtcatacaa ctgccagtcg agctggtatt
     1261 tcacctgctg tttagcacgg cgtaacaacg gcgtgtcggt aatcgcattg cgcgcatcga
     1321 cataatcata actcacagta tgcgtcagtg gtccggtatc aaaattggcg gtcgcctcga
     1381 cgcccttaat ccgcgctttc ccttcgttgt aatatttcag ggtgtgatca tcataatcga
     1441 tcaagtcact gacatcgtta cgatatccgg aaatacgcca gttcacccca gcggttaagc
     1501 cttcaaacgc gccttcccac tgtttgcttt tctccgggtc cagattcgga tttccgtaga
     1561 agccatacag ttgccccaga tttggtgcct tataagatgt cccgtaggaa gcaatgaagc
     1621 gataaccttc gatgaattcc caaccggcgc tggtttgcca ggttccatga cgaccaaact
     1681 gtgagttatc gtcactgcgt gctgcgcctt caaaggtaaa atcgccgact tgttgcagcc
     1741 cggtcagata gatgccggta ttacgttgat catatccatc ctcaacataa cctgtacccg
     1801 gcgtcgtagt ctgtttctgc cagtcgacac ccgcaccaat actaccgtga ccaacgatga
     1861 cattgtttgc ccactggacg gtgtattgct tcatctcatc gagcgtcgcc gacgaatcat
     1921 aacgaccata atggggatcg tagttgtaat ctttgctatg gctatagctg gtaatgagtt
     1981 gtgatttaat cagttcgccg ttatagcgca gcccggcgtc ccaactttgg ctatagagtt
     2041 tacggatatc gagcaacggt gaaccgggag aataatacgc gtcataattg gtacggttat
     2101 catagccata gccgcgcaca aagccgctcc aggcatcagt aaagttatgc tccagcgcgc
     2161 cataaagcgt tttacttaaa aaaacatcgt tatctgtctg cgcttgcgtt ccggtattac
     2221 cataggcaac aacatcataa ccatgagtat gggcataatc gcccaacagc gttacccgtg
     2281 tcttatcccc cagttgttgc tgcgtagaga catcatagtt ctgataacta ttgcttcccc
     2341 accctgctga aatttccgtt ccgggttcat cgcgcgtcgt gatgatattc accaccccgc
     2401 ctattgcatc ggaaccataa acagcggagc gcggcccacg gatatattca acacgctgga
     2461 caagcgcacg caatagggaa ctggctaagg tcggcagaac cactcacccc cgccagattc
     2521 aggcgtacgc catcaattaa caccaacaca tgactggcat ttgtaccgcg aataaaaata
     2581 gatgagagct gacctgaacc gccgttttgg gtgatatcga cgcccggaag acggcgcagc
     2641 acatcattga ccgaggtcga ctgccagcgg tcgatatcct gacgggtcac aacggtggtt
     2701 ggtgcaagca cagtgctgcg cggctgttca aaacggttag cagtaacgac gagagtatcc
     2761 gggctggtat cctgtgccca agcggaaaag gctgtgacag aacacgccgt cagcagcgaa
     2821 gcttttttaa tcattgtaaa gcatccacaa tagaagaagg atgccgcagg tttcatcaat
     2881 attacgcgat gatgagacgt tggccggcag gtcttcgggc ttggaggggt atctaagata
     2941 ctaagagatg atgacttccc accggatggc agtgtccgca taacgcaatc atcgcacctt
     3001 tccttaccgc tgcgcgtcag ctccagattc gcactggatt ccctattaac tcacaggacc
     3061 ggcaagtgga tgctacaggt tgtaacaagt tactgtccag acgtagctca caaataggaa
     3121 ttcatcaaga tctggacatc tgatgagcaa tccctacaat cgccgcgtac tttaattttt
     3181 caggatacat catgaccccc gaacaccttc caacagaaca gtatgaagcg cagttagccg
     3241 aaaaagtggt acgtttgcaa agtatgatgg caccgttttc tgacctggtt ccggaagtgt
     3301 ttcgctcgcc ggtcagtcat taccggatgc gtgcggagtt ccgcatctgg cacgatggcg
     3361 atgacctgta tcacatcatt ttcgatcaac aaaccaaaag ccgtatccgc gtggatagct
     3421 tccccgccgc cagtgaactt atcaaccagt tgatgacggc gatgattgcg ggtgtgcgta
     3481 ataatcccgt tctgcgccac aagttgttcc agattgatta cctcaccacg ctgagtaatc
     3541 aggcggtggt ttccctgcta taccataaga agctggatga tgagtggcgt caggaagcgg
     3601 aggccctgcg cgatgcactg cgcgcgcaga atctgaatgt gcatctgatt ggtcgggcaa
     3661 cgaaaaccaa aatcgagctg gatcaagatt acatcgacga gcgtctgccg gtcgcaggga
     3721 aagagatgat ctaccgtcag gtagaaaaca gctttaccca gccgaacgcg gcgatgaata
     3781 ttcagatgct ggaatgggcg ctggacgtaa ccaaaggctc aaaaggcgat ttactggagc
     3841 tgtactgcgg caacggtaac ttttcattag cgctggcgcg caattttgat cgggtattag
     3901 ccaccgaaat cgctaagccg tcggttgctg ctgcgcaata caacatcgca gctaaccata
     3961 ttgataacgt acaaattatt cgtatggcgg cagaagaatt tactcaggcg atgaatggtg
     4021 tgcgcgagtt taaccgcctg caagggatcg acttaaagag ttatcagtgc gaaaccattt
     4081 ttgtcgaccc accgcgcagc ggtctggaca gtgaaaccga gaaaatggtg caggcgtatc
     4141 cgtgtatttt gtacatctcc tgtaacccgg aaacgttatg caagaatctg gaaacattaa
     4201 gccagacgca caaggtcgaa cgtctggctc tgtttgatca gttcccctac acgcaccata
     4261 tggagtgcgg cgtattactg accgcgaagt aaaacccatg ccggatgcgc cagcatccgg
     4321 cataataccg attactcagc aacttcctgc ttacggttac gcatcttcgc gccaatccag
     4381 aacaccataa tgacggagag tactgccggg aagaagttag agccgatatc cggatattcc
     4441 gcacgaacta ccgtgctgta cagcagtacg ccgagaataa aacaggcagc tgccagaccc
     4501 ggcaagccta ccggcatagt gcgattaaga taacgttgat gcaggcagta aaccgtcagc
     4561 accagggaaa taatcgggaa tacagaaaat ggcacaatgg agctaaacag cgctgcgaaa
     4621 gtaccattaa tcgataagcc agcaactaac gccagcagca gcgtacctct atcttgattt
     4681 gcttgtttca ttactcgtcc ttcacatttc ccggaataat tgcggttttc tcttgttcac
     4741 ggcgatacca gtaataagcc cctttcgaaa tcattcgcag ttgcagtacc agtcgctctt
     4801 ctaattgccg acgttgttcg acgccgacgt ccaacgcctc ggcacccgca ctgaagacaa
     4861 ttgtcaccat tgcttcggct tgcgcttcag taaacgcacg cggcatatgg ttttcgagtt
     4921 ccagatagtc cgcaagttcc gcaatgaagt gctgaatttc acgcgcaacg gcggcacgaa
     4981 acgcagcgga ggtgccggag cgttcccgca ataataaccg gaaggcgtta ggattattac
     5041 cgatgaactc cataaatgtg gagaccgagg tgcggatcac actcccgcct ttggcgatac
     5101 gctgacgcgc ctggcgcatg agttggcgta gcattaaacc gctctcatca accatggtca
     5161 gacccagttc gtctacgtcg cggaaatgcc gataaaaaga ggtgggagca atgcccgctt
     5221 cacgcgccac ttcacgcaaa ctcaggctgg cgaagctgcg ttcagcactt aattggctaa
     5281 atgcggcttc caccagcgaa cggcgggttt tttctttttg ttgcgctctt atgcccatca
     5341 cgatgtctga atccttgcca aaagtactgc tggcactata ccagagaatg aacatactgg
     5401 attgctccag tattgtgaat gaacggtaac gcaataaaac atatttactg gccggttctt
     5461 ttacgtacag cggaaacctg ccgcttaaac ggagagtatc gtcgataaaa atccaataaa
     5521 acgtcagggc aaaagtaaga aacagacaaa gcaaaggccg ctcaggatat agccagataa
     5581 atgacgggga tcaattggct tacccgcgat aaaatgttac cattctgttg cttttatgta
     5641 taagaacagg taagccctac catgccacat tcctacgatt acgatgccat agtaataggt
     5701 tccggccccg gcggcgaagg cgctgcaatg ggcctggtta agcaaggtgc gcgcgtcgca
     5761 gttatcgagc gttatcaaaa tgttggcggc ggttgcaccc actggggcac catcccgtcg
     5821 aaagctctcc gtcacgccgt cagccgcatt atagaattca atcaaaaccc actatacagc
     5881 gaccattccc gactgctccg ctcttctttt gccgatatcc ttaaccatgc cgataacgtg
     5941 attaatcaac aaacgcgcat gcgtcaggga ttttacgaac gtaatcactg tgaaatattg
     6001 caggggaacg ctcgctttgt tgacgagcat acgttggtgc tggattgccc ggacggcagc
     6061 gtcgaaacac taaccgctga aaaatttgtt attgcctgcg gctctcgtcc atatcatcca
     6121 acagatgttg atttcaccca tccacgcatt tacgacagcg actcaattct tagcatgcac
     6181 cacgaaccgc gccatgtact tatctatggt gctggagtga tcggctgtga atatgcgtcg
     6241 atcttccgcg gtatggatgt aaaagtggat ctgatcaaca cccgcgatcg cctgctggca
     6301 tttctcgatc aagagatgtc agattctctc tcctatcact tctggaacag tggcgtagtg
     6361 attcgtcaca acgaagagta cgagaagatc gaaggctgtg acgatggtgt gatcatgcat
     6421 ttgaagtcgg gtaaaaaact gaaagctgac tgcctgctct atgccaacgg tcgcaccggt
     6481 aataccgatt cgctggcgtt acagaacatt gggctggaaa ctgacagccg cggacagctg
     6541 aaggtcaaca gcatgtatca gaccgcacag ccgcacgttt acgcggtggg cgacgtgatt
     6601 ggttatccga gcctggcatc agcggcctat gaccaggggc gcattgccgc gcaggcgctg
     6661 gtaaaaggcg aagccaccgc acatctgatt gaagatatcc ctaccggcat ttacaccatc
     6721 ccggaaatca gctctgtggg caaaaccgaa cagcagctga ccgcaatgaa agtgccatat
     6781 gaagtgggcc gcgcccagtt taaacatctg gcacgcgcac aaatcgtcgg catgaacgtg
     6841 ggcacgctga aaattttgtt ccatcgggaa acaaaagaga ttctgggtat tcactgcttt
     6901 ggcgagcgcg ctgccgaaat tattcatatc ggtcaggcga ttatggaaca gaaaggtggc
     6961 ggcaacacta ttgagtactt cgtcaacacc acctttaact acccgacgat ggcagaagcc
     7021 tatcgggtag ctgcgctaaa tggcttaaac cgcctgtttt aacactttat cgaaatggcc
     7081 atccattctt gcgcggatgg cctctgccag ctgctcatag cggctgcgca gcggtgagcc
     7141 aggacgataa accaggccaa tcgtgcggcg aggttccggc ttaatacacg gcaaataaac
     7201 aaccccatcg cgtttgcgct ccggcggcac agccagcgca ggcagtaaag tgatcccgct
     7261 acctgccgcc accatgttac gcagtgtctc caggctggtc gcgcggaagt gtgtatcttc
     7321 atccgccccg gcttcaaagc agaaacccat tgcctgatcg cgcaaacagt gaccatcttc
     7381 cagcatcagc agtttttccc ctgccagatc ggccatcggt acgcattcgc ggttcgccca
     7441 cgggtgatct tcatagatag ccagcaacat tggctcatca aacaacggca cttcaatgaa
     7501 tgcttcgctc tctttcacca gcgcgaggat cacgcaatcg agtttgccgc tgtccagttg
     7561 cgccagtaac tggtgggtct gtgcttcatg cagatacatt tccagctttg gaaatgtctg
     7621 gtgcagcatc gggataatat gcggcagcag gtacggtcca accgtaggaa tcaaaccaat
     7681 atgcagcggt ccggacatcg tctcgccctg ctggcttgcc atctctttaa ggactttcac
     7741 ctcacgcagc acggtacgcg cctgatccac cagcagcatt cccgcctggg tgaacaacac
     7801 tttacggctg gtccgctcca gcaacatcac gcccagctca tcttccagct tacgaatttg
     7861 gccgctaagc gtcggctggc taacgtggca ggaatctgcc gcacgccgaa aatggcggtg
     7921 ttcagccaat gccaccaggt actcaagatc acgaatattc attatccatc ctccatcgcc
     7981 acgatagttc atggcgatag gtagaatagc aatgaacgat tatccctatc aagcattctg
     8041 actgataatt gctcacagaa acggagcggc acctctttta acccttgaag tcactgcccg
     8101 tttcgagagt ttctcaactc gaataactaa agccaacgtg aacttttgcg gatctccagg
     8161 atccgctttt ttttgccata aaaaagcccg gcgataagcc aggctcaaat ttgtacatat
     8221 aaatgttctt accccaaccg cgcctgcgca aaagcaatcg cttgggccac ctgctgcggt
     8281 gagacgccgc cttttgccgc acgcttgtcg aggcacgatt gtagcgacag aatcggatag
     8341 acatcttcgc caatcacgtg gctgaatttc tgcaactcgt cgagcggcag atcttccagc
     8401 ggtttgccct gacgaatggc ttccaccacc gcttcaccca cgatatggtg cgcctcgcgg
     8461 aaaggtacgc ctttcgccac caggtaatcc gccagttcgg tggcgttggc gtaaccctgc
     8521 tgagccgctt cctggcaacg tggacgtttc acctgaatgc cgtccagcac cagcgccgcc
     8581 atatgcaggc agtccagcca ggtatcgaac gcgtcgaaca gaccttcttt gtcttcctgc
     8641 atatctttgt tgtaagccag cggcaaacct ttcagcgtca tcatcatgcc ggttaacgca
     8701 ccctgcaccc gaccgcattt accgcgaatc agctccagcg catccgggtt tttcttctgc
     8761 ggcattaatg atgaaccgga agtcacgcgg tcagaaagct ccacaaaccc cgcttcgccg
     8821 gtgttaaaga aaatcagatc ttcagcaaaa cgcgacagat gcaccatgcc gatagcggca
     8881 gccgacagca gttccaacac atggtcacgg tcagaaacgc tgtccagact gttacgggtc
     8941 gccgaagcaa agcccagcca gcctgctaac tgttcacggt cgatttcata ggccgttccc
     9001 gccagcgcgc cacagcctaa tgggctgaca tccagacgct taagcgcatc ctgcaaacgg
     9061 ctttcatcac gcgccagcat ctcaacatag gccaggcacc agtgcgcgaa cgtcaccggc
     9121 tgggcgcgtt gcaggtgagt gtaacctggc attaccgcgt cctgattgtt ttgtgcggtt
     9181 tccaccagcg ccgattgcag ctgccggtta gccgtcagta actcgctaac ggtatctttg
     9241 caccacagtt tcaggtcagt cgctacctga tcattacggc tacgcccggt atgcagcttt
     9301 ttgcctaact ggcccacttt gtcgatcagt ttgccttcca cccagctatg gatatcttcg
     9361 gcgtcgcttt caaggatttg ttgtggcctg gcgcgaacat cttccagcaa cacgttcagc
     9421 gcctcttcca gttgcgcctg ctcttctgcg gttaacacgc cgaccgttac cagggctttg
     9481 gaccaggcca cagagccaac aatatcctgc tccgccagac ggtaatcaaa gcgcagtgag
     9541 tcgttgaatt gtttgaaccg ttgatctgct gcctgggtaa aacgcccgcc ccaaagtgcc
     9601 ataactctgt ttccttattt tgaaattcaa tgccggaaag ctgcgcctcc ggctaacaaa
     9661 acttaagcta aaatccgcgt acccatcggc ataccgttaa acagtgccgg aagctgctcc
     9721 gcatgacgcc aggaggcgat atctaccgga cggcccagcg tgcgggccgc atccagcgcc
     9781 gcgttcactt tcactatcat gccgtcagta ataatgccct gctcaatcag ttgttctgct
     9841 ttcgcggcgg tcatttcggc aatgcgttgc cctttgccgt cgagaatgcc gctgacgtcg
     9901 gagagcaaaa tcagatccgc gcccagcgtt gccgccagcg ccgttgccgc ctggtcggca
     9961 ttgacgttca tcagttgccc ttcgtctgtt acgccaatgg agctgaccac cggcagataa
    10021 ccgttctcca gcaaggagtt gataagctta ggcgaacctg gctgcgccag tccaacatga
    10081 cctaactctt catcaagctg ggtcactttg acgctgtcgc catcaccgag aaacaaacct
    10141 acagccgcaa tctgatgttt cttcgcccat gccaacaggg ttttatttgc cgttcccgcc
    10201 agtgctccgg tgataatgtc tatctgatca gcaggcgtca cccgcaggcc gtttttcttt
    10261 ttcaccggca gattcagccc tttcatcagc tcatccacca cgcaaccgcc gccgtgcaca
    10321 atcaccagcg gacgctgatg tgactcacga taattcacca gtgcgctaaa cagacgttcc
    10381 agcgcctctt cactatccag cagtacgccg cccagtttga taattaatgg attcatcatt
    10441 gcacccttaa ataagagact gcgtttccgc atagccgaaa cgaatattgg cgcactgtac
    10501 cgcttgtgcc gccgcgcctt tcagtaagtt gtcttcggtc gccacaataa tcagatgctc
    10561 gccctgaacg gcaaacccga tatcgcaaaa tggcagccca acgacatttt tcagcgccgg
    10621 aacgcctttg tcatacagcc gcaccagcgg tttatgggca tacgcctgct gtaacacttg
    10681 cgcgacttgc gcctgggtca cacccgattt caggcggcag gtaatggttt cgagaatgcc
    10741 gcgcgggaaa ttgcccagat gtggggtgaa gataacgtca gcaccgaggt gtgtggcgat
    10801 ctctggttga tggcgatgag taaagacgcc atacggttgc aggctaactt cacaaaaact
    10861 atttgaaatc gctgccttac gtcctgcacc gctcacgccg ctggtggcgt taatcgccgg
    10921 ccactgattg aggtcaagaa gatcggcatc aatcaacggt ttcagcgcca gctgtgccgc
    10981 cgtcggataa cagcctggca ccgcaatcaa attcgcttct tttaatttat taccgcacca
    11041 ctccgccaga ccgtaggctg cctgttccag cagttccggg tattgatggg taaagccgta
    11101 atatttttca tagaaggtgg cgtcgttaac acgaaacgcg ccggaaaggt cgaacaccac
    11161 gcagcccgct tcaagaaact gcggcgctaa atcgtggcta acttcatggg cggtggcgag
    11221 aaacactacg tccacccctg ggctaaactc gctgatatcc gacatcggct gcaacggcag
    11281 ctcaacgatg ccttttagct gcggatgcaa atcggagatt aactttcccg catcattgct
    11341 ttgcgctgaa acagtcaaag cggttatgtt catatgcgga tggcgattta cataggtcac
    11401 tagctctgcg ccagcgtagc cgctggcacc cacaatcagc gtattcaaca tcggggctat
    11461 tcaccttctt atgtctggtt gccaggttaa acgtaaaaca ttcaccttac ggctggtggg
    11521 ttttattacg ctcaacgtta gtgtattttt attcataaat actgcatgaa tattgatact
    11581 atcatgacca gaggtgtgtc aacaatgaaa aacaaattac cgccatttat cgagatttac
    11641 cgcgctctga ttgccacacc ttcaataagc gctacggaag aggcactcga tcaaagcaat
    11701 gcagatttaa tcactctgct ggcggactgg tttaaagatt tgggcttcaa tgtggaagtg
    11761 cagcctgttc caggaactcg caacaaattc aatatgctgg caagtatcgg acagggggct
    11821 ggcggcttgt tgctggcggg gcataccgat acggtgccat ttgatgacgg tcgctggacg
    11881 cgcgatccgt ttacactgac ggagcatgac ggcaagcttt acggcttagg caccgccgac
    11941 atgaaaggct tttttgcgtt tatccttgat gcgctacgcg atatcgacgt cacgaaactg
    12001 aaaaaaccgc tctacattct ggcgactgct gatgaagaaa ccagtatggc cggagcgcgt
    12061 tattttgccg aaactaccgc cctgcgcccg gattgcgcca tcattggcga accgacgtca
    12121 ctacaaccgg tacgcgcaca taaaggtcat atctctaacg ccatccgtat tcagggccag
    12181 tcggggcact ccagcgatcc agcacgcgga gttaacgcta tcgaactaat gcacgacgcc
    12241 atcgggcata ttttgcaatt gcgcgataac ctgaaagaac gttatcacta cgaagcattt
    12301 accgtgccat accctacgct caacctcggg catattcacg gtggcgacgc ttctaaccgt
    12361 atttgcgctt gctgtgagtt gcatatggat attcgtccgc tgcctggcat gacactcaat
    12421 gaacttaatg gtttgctcaa cgatgcattg gctccggtga tcgaacgctg gccgggtcgt
    12481 ctgacggtcg acgagctgca tccgccgatc cctggctatg aatgcccacc gaatcatcaa
    12541 ctggttgaag tggttgagaa attgctcgga gcaaaaaccg aagtggtgaa ttactgtacc
    12601 gaagcgccgt ttattcaaac gttatgcccg acgctggtgc tcggtccggg ttccatcaat
    12661 caagctcatc agcctgatga atatctggag acgcggttta ttaaacccac ccgtgagttg
    12721 attatccagg taattcacca tttttgctgg cattaaaacg taggccggat aaggcgctcg
    12781 cgccgcatcc ggcaccgttg ccaaactccg gtgccgcaat aatgtcggat gcgatgcttg
    12841 cgcatcttat ccgacctaca cctttggtgt tatgtatggc gattttttaa catttccata
    12901 agttacgctt atttaaagcg tcgtgaattt aatggcgtaa attcctgcta tttattcgtt
    12961 tgctgaagcg atttcgcagc atttgacgtc accgctttta cgtggcttta taaaagacga
    13021 cgaaaagcaa agcccgagca tattcgcgcc aatgcgacgt gaaggataca gggctatcaa
    13081 acgataagat ggggtgtctg gggtaatatg aacgaacaat attccgcatt gcgtagtaat
    13141 gtcagtatgc tcggcaaagt gctgggagaa accatcaagg atgcgttggg agaacacatt
    13201 cttgaacgcg tagaaactat ccgtaagttg tccaaatctt cacgcgctgg caatgatgct
    13261 aaccgccagg agttactcac caccttacaa aatttgtcga acgacgagct gctgcccgtt
    13321 gcgcgtgcgt ttagtcagtt cctgaacctg gccaacaccg ccgagcaata ccacagcatt
    13381 tcgccgaaag gcgaagctgc cagcaacccg gaagtgatcg cccgcaccct gcgtaaactg
    13441 aaaaaccagc cggaactgag cgaagacacc atcaaaaaag cagtggaatc gctgtcgctg
    13501 gaactggtcc tcacggctca ccctaccgaa attacccgtc gtacactgat ccacaaaatg
    13561 gtggaagtga acgcctgctt aaaacagctc gataacaaag atatcgctga ctacgaacac
    13621 aaccagctga tgcgtcgcct gcgccagtta atcgcccagt catggcatac cgatgaaatc
    13681 cgtaagctgc gtccaagccc ggtagatgaa gccaaatggg gctttgccgt agtggaaaac
    13741 agcctgtggc aaggcgtacc aaattacctg cgcgaactga acgaacaact ggaagagaac
    13801 ctcggctaca aactgcccgt cgaatttgtt ccggtccgtt ttacttcgtg gatgggcggc
    13861 gaccgcgacg gcaacccgaa cgtcactgcc gatatcaccc gccacgtcct gctactcagc
    13921 cgctggaaag ccaccgattt gttcctgaaa gatattcagg tgctggtttc tgaactgtcg
    13981 atggttgaag cgccccctga actgctggcg ctggttggcg aagaaggtgc cgcagaaccg
    14041 tatcgctatc tgatgaaaaa cctgcgttct cgcctgatgg cgacacaggc atggctggaa
    14101 gcgcgcctga aaggcgaaga actgccaaaa ccagaaggcc tgctgacaca aaacgaagaa
    14161 ctgtgggaac cgctctacgc ttgctaccag tcacttcagg cgtgtggcat gggtattatc
    14221 gccaacggcg atctgctcga caccctgcgc cgcgtgaaat gtttcggcgt accgctggtc
    14281 cgtattgata tccgtcagga gagcacccgc cataccgaag cgctgggcga gctgacccgc
    14341 tacctcggta tcggcgacta caaaagctgg tcagaggccg acaaacaggc gttcctgatc
    14401 cgcgaactga actccaaacg tccgcttctg ccgcgcaact ggcaaccaag cgccgaaacg
    14461 cgcgaagtgc tcgatacctg ccaggtgatt gccgaagcgc cgcaaggctc cattgccgcc
    14521 tacgtgatct cgatggcgaa aacgccgtcc gacgtactgg ctgtccacct gctgctgaaa
    14581 gaagcgggta tcgggtttgc gatgccggtt gctccgctgt ttgaaaccct cgatgacctg
    14641 aacaacgcca acgatgtcat gacccagctg ctcaatatcg actggtatcg cggcctgatt
    14701 cagggcaaac agatggtgat gattggctat tccgactcag caaaagatgc gggcgtgatg
    14761 gcagcgtcct gggcgcaata tcaggcacag gatgcattaa tcagaacctg cgaaaaagcg
    14821 ggtattgagc tgacgttgtt ccacggtcgc ggcggttcca ttggtcgcgg cggtgcacct
    14881 gcccatgcgg cactgctgtc acaaccgcca ggaagcctga aaggcggcct gcgcgtaacc
    14941 gaacagggcg agatgatccg ctttaaatat ggtctgccag aaatcaccgt cagcagcctg
    15001 tcgctttata ccggggcgat tctggaagcc aacctgctgc caccgccgga gccgaaagag
    15061 agctggcgtc gcattatgga tgaactgtca gtcatctcct gcgatgtcta ccgcggctac
    15121 gtacgtgaaa acaaagattt tgtgccttac ttgcgctccg ctacgccgga acaagaactg
    15181 ggcaaactgc cgttgggttc acgtccggcg aaacgtcgcc caaccggcgg cgtcgagtca
    15241 ctacgcgcca ttccgtggat cttcgcctgg acgcaaaacc gtctgatgct ccccgcctgg
    15301 ctgggtgcag gtacggcgct gcaaaaagtg gtcgaagacg gcaaacagag cgagctggag
    15361 gctatgtgcc gcgattggcc attcttctcg acgcgtctcg gcatgctgga gatggtcttc
    15421 gccaaagcag acctgtggct ggcggaatac tatgaccaac gcctggtaga caaagcactg
    15481 tggccgttag gtaaagagtt acgcaatctg caagaagaag acatcaaagt ggtgctggcg
    15541 attgccaacg attcccatct gatggccgat ctgccgtgga ttgcagagtc tattcagcta
    15601 cggaatattt acaccgaccc gctgaacgta ttgcaggccg agttgctgca ccgctcccgc
    15661 caggcagaaa aagaaggcca ggaaccggat cctcgcgtcg aacaagcgtt aatggtcact
    15721 attgccggga ttgcggcagg tatgcgtaat accggctaat cttcctcttc tgcaaaccct
    15781 cgtgcttttg cgcgagggtt ttctgaaata cttctgttct aacaccctcg ttttcaatat
    15841 atttctgtct gcattttatt caaattctga atataccttc agatatcctt aaggaattgt
    15901 cgttacattc ggcgatattt ttttcaagac aggttcttac tatgcattcc acagaagtcc
    15961 aggctaaacc cctttttagc tggaaagccc tgggttgggc actgctctac ttttggtttt
    16021 tctctactct gctacaggcc attatttaca tcagtggtta tagtggcacc aacggcattc
    16081 gcgactcgct gttattcagc tcgctgtggt tgatcccggt attcctcttt ccgaagcgga
    16141 ttaaaattat tgccgcagta atcggcgtgg tgctatgggc ggcctctctg gcggcgctat
    16201 gctactacgt catctacggg caggagttct cgcagagcgt tctgtttgtg atgttcgaaa
    16261 ccaacaccaa cgaagccagc gagtatttaa gccagtattt cagcctgaaa attgtgctta
    16321 tcgcgctggc ctatacggcg gtggcagttc tgctgtggac acgcctgcgc ccggtctata
    16381 ttccaaagcc gtggcgttat gttgtctctt ttgccctgct ttatggcttg attctgcatc
    16441 cgatcgccat gaatacgttt atcaaaaaca agccgtttga gaaaacgttg gataacctgg
    16501 cctcgcgtat ggagcctgcc gcaccgtggc aattcctgac cggctattat cagtatcgtc
    16561 agcaactaaa ctcgctaaca aagttactga atgaaaataa tgccttgccg ccactggcta
    16621 atttcaaaga tgaatcgggt aacgaaccgc gcactttagt gctggtgatt ggcgagtcga
    16681 cccagcgcgg acgcatgagt ctgtacggtt atccgcgtga aaccacgccg gagctggatg
    16741 cgctgcataa aaccgatccg aatctgaccg tgtttaataa cgtagttacg tctcgtccgt
    16801 acaccattga aatcctgcaa caggcgctga cctttgccaa tgaaaagaac ccggatctgt
    16861 atctgacgca gccgtcgctg atgaacatga tgaaacaggc gggttataaa accttctgga
    16921 tcaccaacca gcagacgatg accgcccgca ataccatgct gacggtattt tcgcgccaga
    16981 ccgacaagca gtactacatg aaccagcaac gtacgcagag tgcgcgtgaa tacgacacca
    17041 acgtgctgaa gccgttccag gatgtgctga atgaccctgc gccgaagaaa ctgatcatcg
    17101 ttcatctgct gggtacgcat atcaaataca aataccgcta cccggaaaat cagggcaagt
    17161 ttgatggcaa taccgatcat gttccgccag gattaagcgc agaagagctg gaatcatata
    17221 acgattatga caacgctaac ttgtataacg atcatgtggt tgccagcctg attaaagact
    17281 ttaaagcggc agacccgaac ggattcctgg tttacttctc tgaccacggt gaagaggttt
    17341 acgacacgcc gccgcacaaa actcaggggc gtaacgaaga caacccgacg cgccacatgt
    17401 acaccattcc gttcctgctg tggacgtcgg aaaaatggca agcgactcat ccccgtgatt
    17461 tctcacagga tgtcgatcgt aaatacagcc tggcggaact gatccacacc tggtcagatt
    17521 tggcgggctt atcttacgac ggttacgatc caacccgttc agtggtgaat ccgcagttca
    17581 aagaaactac ccgctggatt ggtaacccgt acaagaaaaa cgcgctgatc gattacgaca
    17641 ctctgccgta tggcgaccag gtaggtaatc agtaattatc agccaggcgc tatttgccgg
    17701 atgcgacgct tgacgcgtct tatccggcct acagatcgcg gcggtaggct ggataaggcg
    17761 tttacgccac atccggcaat acaactgccg caatgtttac atcgaactcc cctcaggcca
    17821 cacttttcag gtggcctttt ttaataatgc cagttactgt tttcccgtct acatacctaa
    17881 gggaataaca tgtatcacga catcagctat ctgctctccc gcctgatcaa cggcccgctg
    17941 tccctgcgcc agatttactt tgccagcagt aacggcccgg tgcccgatct tgcgtatcag
    18001 gtcgattttc cacggctgga aattgtgctg gaaggtgagt ttgttgatac cggcgctgga
    18061 gcaacgttag ttcccggcga tgtgctgtac gttgccgctg gtggctggaa tttcccacaa
    18121 tggaaaacac ccactaccac ctttagcgtg ctgtttggca aacagcaact cggcttcagc
    18181 gtcgtgcaat gggatggcaa acaatatcaa aatctggcga agcaacacgt cgcccgacgc
    18241 ggcccacgta taggttcttt tctgctacaa acgctcaatg aaatgcaaat gcagccgcag
    18301 gagcagcaaa cggcaaggct tatcgtagcc agcctgctta gccactgccg cgatttgctc
    18361 ggcagccaaa tccagaccgc ctcacgcagc caggcactat ttgaagctat tcgcgattat
    18421 atcgacgaac gctatgcctc cgcgcttacc cgcgaatctg tcgcacaggc attctatatt
    18481 tcaccaaact atctctcgca cctgtttcaa aaaacggggg ccattggttt taacgaatac
    18541 ctgaatcaca cgcgactgga gcacgctaag acgttattga aaggctatga cctgaaggta
    18601 aaagaagtgg cgcacgcctg cggctttgtc gacagcaact acttctgccg tctgtttcgt
    18661 aaaaacaccg aacgctcgcc gtcggaatat cgccgacagt accacagcca actgacagaa
    18721 aaaccgacta ctccagaata agatgggttt gctgcggcgc agaaagcact ttgcgcaccg
    18781 cgctcattac ccgctgcggc tcacgcagga atgcgtagat gctgcattgc acatagcggc
    18841 aatgttcaaa tcgctcggca ccggcaagct cgatatccgt aatcaacaga gcaacatccg
    18901 cccgacggat atcctcgtcc gctaaacgat tctccgttcc cagcgcgccc tgagtttcaa
    18961 tgctgacgcc ccacttctct aactggcaca acttttccag ccgttccgcc gccatataag
    19021 tatgcgccac gccgcttacg caggcggtta ctgccaccag gtatgccatt ttaacctccc
    19081 acggtaacct gaaatccggc ccgttctgcc atttcgcgca tcgttgccac atcggctgat
    19141 gacggcgcag caacatcctt catcgaccat gttttcccca gcaggcggta tttcggctcg
    19201 ccgtactgat gaaacggcaa cagatggatc tgcctgatat tcagcgggat cagcacgtcc
    19261 agcgcctgct gcatattctc ccggctgagt gtgaaaccgg ggatcagcgg caaacgcggg
    19321 atcacgttga caccctcact caccagcaaa cgcaggttct ccagtacgcg tggcaggttc
    19381 atcttcacca catcccgcgc ctgagtcgcg tccataattt ttaaatcgaa caacacttca
    19441 tcgcacaatt tcgccagcgg taacagcttg ctggctggtg cgtctccggc agtttcaatg
    19501 gcacatgaga caccccacag ccgcagtcgc tgtaaaaaac gggtagcaaa ctccgcctgc
    19561 attaacactt cgccgccaga aagcgtgacg ccgccgccgg acgtgcgaaa aaaaatgtca
    19621 tctttcatca cttcccgttc cagagcgtca aggctgatat cgcgaccaat ccgttcaaac
    19681 gccccggagg ggcattcatc cgcatcacgc aaacatttcg cacagtgcag acatttcgcc
    19741 tctctgcgta ccgtctggat tttgccggag atcgactccg gattagcaca ccacgggcaa
    19801 agatgcggac agcctttaaa aaagaccacc gtacgaatgc cctcgccgtc attcagtgaa
    19861 taacgctgaa tgttgaaaat gcgcgccaca tcattgaggc gcgtttccac aacgttacag
    19921 ctgatgcgct gtccggcgga tgatgtcatc ctggatctcc ttcgacagtt cgacaaagaa
    19981 ggcgctgtat ccggcaacgc gcaccaccag cccggcataa tcttgtgggc gctgttgcgc
    20041 ttcccacaac gtgtcggcgt tcaccacgtt aaactgaata tgctgcaact taagctgggt
    20101 aaacgcccgt aagaagtcgg ccagtttgcg taatcccgct tcaccttcca gggtcgccgg
    20161 agtgaatttc acgttcagca acgtaccgtt agacagcagc gtattatcga gcttactgac
    20221 tgacttcagt accgctgttg gcccttgttc gtcctggccc agcataggtg acaagccgcc
    20281 gtctgccagc tgttctccgg caaaacgacc gtctggcgtc gcgccaacca ccgatcccaa
    20341 cgggacgtga gcagaaacgg tatacgatcc cggcgtgaag taaccaccgc gcgggttctg
    20401 gtatttttcc acttctttgc agtagtggcg caacagttcg gcgctaatgt tatccacctc
    20461 gtcgatatcg ttaccatatt tctcaaagcg gttaattaag cgagcgcgga ctttttcgcc
    20521 ttctggcgtt gcaaagttgg cttttaatac cgacagcaat tcgtcaaaac ttaaacgctg
    20581 ttgctcaaaa accatccctt tcaacgcatg gagagaatcg ctcaggttgg cgataccgat
    20641 cccctgtacg ccggagaagt tataacgcgc gccgccatcg gtaatatcgc gacctttttc
    20701 cagacaatcg ctgataaaag acgagagcag cggtacaggt gcccagtcgc gatggccgat
    20761 atcacaaata ttgctgccct caaccatcag ggtgatgtag tggctgatct tggcgcggat
    20821 ctgctccagt aaaccttcat aagtcagcgc agcattgcct tcattttcat gcaggcagat
    20881 ttccatcact ttcagcagat taaacatcgc gatgtcatgc aagccgtagg ttctgccggg
    20941 aatagataat tccacacagc ccactacgga atagtcgcgc gcatcttcca gcgaaacgcc
    21001 acggttgagg aacgctggta ccaccacttc atcgttaaag atttgcggaa taccggtgcc
    21061 gaggcgaatg gtttcggcgg ttttcatcag gaatggcgtg tcgatcaagg cgttagtgcg
    21121 cacgccgagg ttcggttgcg gtaattgcac gctttgatag gcgtcaaggc aaaggaacga
    21181 aagcacgttc accgcgctac gtccgttctc ggttaacccg ccgagcagtg cggtatagcc
    21241 ggtcgggaaa cctgcgaaat aacgcgcgct gctggtggag cgcaacagca cgatgtcgtt
    21301 gcatttcacc cataaagatt cgagcagttc tttcaggaac gccggatctt cgccctgggt
    21361 taaagatgcc tgatagaacg gcaacatata ctggtcgaag cgccctaaag acagcgaact
    21421 ggcgttggat tcgtattgca gaatgatgtt catgtaccag aataactggc acgcctgcca
    21481 gaacgtctgc ggcttatgtt cggcgttatg gcgagagatc tccgcaatag tcagcagttc
    21541 ttcgcgacgc tgggcatctg tgcagtttgc cgccatcgtt tccgccagta cagcgtaacg
    21601 caaaatgtgt ttctgcgagg cttccagcag taacagcgct gcctgataaa agtgattctc
    21661 cggctgttgc tgacaatgtt gctgcatctg tgccaccaac tcccccagcc cgtgattcaa
    21721 caggcgtggg taatcaataa taatgtgccc ctggccttta tccgtctggt taatgctgaa
    21781 aatctgcgtg ctggtcgcgg cttttacttc atccgtcatc tgcccgttga tgaaatcttt
    21841 catcgaacgt ttttcccagt acgggaacaa ctcttcacga tagatacgtt tatcttcttc
    21901 gctgatagca aagcggtcct gcggacgcgt cgggaattga tccagctctt tcagcagcca
    21961 gtaagggtcc atttccggcg acataatccc ggcgcgcggt tttacggtgc ggttaccggc
    22021 aatcagttct tcatcacgaa tcgaaatttc aacatgttca aggatatacg ctgttgcttt
    22081 cgcccggcgc aatatcaccg gttcgccttc ggtttgccga tggctggcgg tataaagcag
    22141 cgcccgctcc agcgagattt cacgggtatt ggcaaacagt gcagttttga ggcgagagat
    22201 acgattcgtc atgaggaact cctgtgggaa agcgaacccg cccttgcgag cgggagaaga
    22261 acttaagcgg tttgcgccag gtgagcttca attttgctca taatggcgtc agcacgtttt
    22321 actgcatcgc tgatgttgac gcgcacgatg gtcttgccag caaaacgctc ttcaaacttg
    22381 atgccgatat ctttggtcag aatcaccata tcagcgctcg ccacatcttc cgcagtcagt
    22441 tcattttcca gaccaattga cccctgggtt tctactttca cttcccagcc tttcgctttc
    22501 gcggcacttt cccgcgcctc tgctgccata taggtatggg caacacctga aggacatgcg
    22561 gttactgcaa taattttcgt catggctcgt tcctcaatta attaatttca aaatccaaat
    22621 ccaggtcgtc ttctttttca tcagtcgaag acccattttt acgcgccaga cttttcagca
    22681 cgttcacaca aacagccgtc accactgctc cgacagccac tgctgcgata taacccagct
    22741 tgccttcaac caccggcagc acaatcagac caccccagcc tgcatagcac tgcgcaccga
    22801 ccagcgccgc agttactgca ccacaaactg aaccgaccat gatggaagga atgacacgca
    22861 gcggatcggc agcggcgaaa ggaatcgccc cttcagtaac gccaacacag cccatcacca
    22921 gtgccgcttt gccggtttcg cgctcttctg cggagaaatt tttacgacca atcagcgtcg
    22981 ccagccccat ccccagcggc gggatacaaa tacccacggc agcgatgacc accacggtat
    23041 aaacaccctg agcaacgcaa atcagcatga aggcataggc tactttgtta accggaccgc
    23101 ccatatcgaa cgccagcatc agacccatga tcaccgccag cataacaatg ctgccctgct
    23161 gcatcccctg aagccactga gtcaggctgt tggtcaacgc ccctacaggc tcgcccaagc
    23221 cccacatcat gatgcctgcg gtaatcagtg tgccgacgat aggaataatg aagataggca
    23281 tcaccgagcg cagaacttta tgcaccggaa ttttcttcag gtaatgcacc acgatgccgc
    23341 cgataatccc ggcgatcagt gcaccaaaga agcccgcacc aaagctgtta ccaacccagg
    23401 caccgatagc gcacggagcc agcgcagaac gctctgcaat ggagtaacca atgtaagcgg
    23461 cgaggaaagg caccatcagc gtcaaacctg caacgccgat atcaaacagt tttttcaaat
    23521 tcggatcggc tacggcatcc ggcactgcgc ctttgccata caacatgacg gaaaccgcca
    23581 gcaaaatacc gcccgatacc acgaagggaa tcatgtgtga aacgcccgtc attaaatgct
    23641 gacgggtatt tttcaggatc tgcaccaact cattcataag ctgctcccgg gcttgcgaaa
    23701 gcccatgtcc atatttttga tggaggaaac aataggcaat cgcaggcgga gcaaacagtg
    23761 gataaaaagg ccatttactg gacttttgta atatcagtac aaaaatgcga tccgcctcat
    23821 aacttgcgat aaaccggaag catgccgcaa aacggcgtct tgtgagacgc gtcgcaaatt
    23881 gacggttaca ttacatttgt ccagcttttg gcagatttgt cacatggcag gccgttgccg
    23941 tgcgctctta tcctgtaaac agaaaaccgt accgggagag aggcaatggc cctgattgtg
    24001 gaatttattt gtgagctacc taacggcgta catgcgcgtc cggcaagcca cgttgaaacg
    24061 ctgtgtaata ctttttcatc acaaattgag tggcataacc tgcgcactga ccgcaagggc
    24121 aacgccaaaa gcgcccttgc gctgattggc accgatacgc tggcgggcga taactgccag
    24181 ttactgattt ccggggccga cgaacaggaa gcgcaccagc gtttaagcca atggctgcgc
    24241 gatgaattcc cccactgcga tgcgccgctg gcggaagtta aatctgacga actggaacca
    24301 ctgccggttt cactgaccaa tctgaatccg caaattatcc gcgcccgcac cgtgtgcagc
    24361 ggtagtgcag gcggcattct gacgccgatc tcttctttag atctcaatgc gctgagtaat
    24421 cttcccgcag ccaaagacgt tgacgccgag caatccgcac tggaaaatgg cctgacgctg
    24481 gtactgaaaa acattgagtt tcgtctgctg gatagcgacg gtgctaccag cgcgatactg
    24541 gaagctcacc gatccctggc tggcgataca tctttacgtc agcacttact gacaggcgtc
    24601 agcgagggat taagctgtgc cgaagcgatt gtcgccagtg cgaatcactt ttgcgaagag
    24661 ttcgcccgtt ccagcagcag ctacctgcaa gaacgtgccc tggacgtacg cgacgtctgc
    24721 ttccagttac tccagcaaat ctacggtgag caacgcttcc cggcaccggg caaactgacg
    24781 cagcccgcca tttgtatggc tgatgaactg actcccagcc agttcctcga actggataaa
    24841 aatcacctca aaggtctgtt actgaaaagt ggcggcacca cctcacatac cgtaattctt
    24901 gcccgttcgt tcaacattcc gacactggtt ggtgtagata ttgatgccct tactccgtgg
    24961 cagcatcaaa cgatttatat cgacggcaac gccggggcga ttgtggttga gccaggggaa
    25021 gccgtggccc gttattatca gcaagaagcc cgcgtacagg acgcccttcg tgagcaacag
    25081 cgtgtctggc tgacccaaca agcccgtacc gctgacggta tccgcattga aattgccgct
    25141 aacatcgctc actccgtgga agcgcaggcc gcattcggca atggtgcgga aggcgttggt
    25201 ttgttccgca ctgaaatgct ctatatggat cgcaccagtg caccgggcga aagcgagctg
    25261 tacaacattt tttgtcaggc gctggagtct gccaacggac gcagcattat tgtgcgcact
    25321 atggacattg gcggcgacaa acccgttgat tatctgaaca ttccggcaga agctaacccg
    25381 ttcctcggtt atcgcgctgt gcgtatttat gaagaatacg cctcgctgtt caccacacaa
    25441 ctacggtcga tcctgcgcgc ctccgctcac ggcagcctga aaatcatgat cccgatgatc
    25501 tcctcaatgg aagagatctt atgggtgaaa gaaaaactgg cggaagccaa acagcaactg
    25561 cgtaacgaac acattccgtt tgatgagaag atccagcttg gcattatgct ggaagtaccg
    25621 tcggtgatgt tcatcatcga tcaatgctgc gaagagattg atttctttag tattggtagt
    25681 aatgacctga cgcaatatct gctggcagtg gatcgcgata acgctaaggt tactcgtcac
    25741 tacaacagcc tgaatccggc attcttgcgg gcgctcgatt acgccgtgca ggcggtgcat
    25801 cgccagggca aatggattgg tctgtgcggt gagctgggag cgaaaggttc cgtgctgccg
    25861 ttgctggtcg gcttagggct ggatgaactc agcatgagcg caccatcaat tccggcggcg
    25921 aaagcgcgga tgacgcaact tgatagccgt gagtgccgca agttgctcaa ccaggcaatg
    25981 gcctgccgta cttcgctgga agtggaacac ctgctggcgc aattccgcat gacccaacag
    26041 gacgcaccgc tggtcaccgc cgagtgcatc acgctggaaa gtgactggcg cagcaaagaa
    26101 gaagtgctca aaggcatgac cgataacctg ctgctggcgg gccgctgccg ctatccgcgt
    26161 aaactggaag ccgacttatg ggcgcgcgag gccgttttct ctaccggtct gggctttagt
    26221 tttgccattc cacacagcaa atcagaacac attgagcaat ccaccatcag cgtggcgcgt
    26281 ctgcaagcgc cggtgcgctg gggcgatgat gaagcgcaat tcatcattat gttaaccctg
    26341 aacaaacacg ctgcgggcga tcagcacatg cgcattttct ctcgcctcgc tcgccgcatc
    26401 atgcacgaag aattccgtaa cgcgctggtt aacgccgcct ctgccgacgc tatcgccagc
    26461 ctgctgcaac atgaactgga actgtaaaag gaaacatcat ggaactgtat ctggacaccg
    26521 ctaacgtcgc agaagtcgaa cgtctggcac gcatattccc cattgccggg gtgacaacta
    26581 acccgagcat tatcgctgcc agcaaggagt ccatctggga agttctgccg cgtctgcaaa
    26641 aagcgattgg tgatgagggc attctgtttg ctcagactat gagtcgtgac gcgcagggta
    26701 tggtggaaga agcgaagcgc ctgcgcgacg ccattccggg cattgtggtg aaaatcccgg
    26761 tgacttccga aggtctggcc gccattaaga tgctgaaaaa agagggtatt actacgcttg
    26821 gcaccgcagt gtacagtgct gcacaagggt tactggccgc gctggcaggg gcaaaatacg
    26881 ttgctccgta tgttaaccgc gtagatgccc agggcgggga cggcattcgt acggttcagg
    26941 agctgcaagc gctgttagaa atgcacgcac cagaaagcat ggtgctggcg gccagcttta
    27001 aaacgccgcg tcaggcgctg gactgtttac tggcaggatg tgaatccatc accctgccct
    27061 tagatgtagc gcaacaaatg ctcaacaccc ctgcggtaga gtcagctata gagaagttcg
    27121 aacacgactg gaatgccgca tttggcacta ctcatctcta aaggagcaat tatggaccgc
    27181 attattcaat caccgggtaa atacatccag ggcgctgatg tgattaatcg tctgggcgaa
    27241 tacctgaagc cgctggcaga acgctggtta gtggtgggtg acaaatttgt tttaggtttt
    27301 gctcaatcca ctgtcgagaa aagctttaaa gatgctggac tggtagtaga aattgcgccg
    27361 tttggcggtg aatgttcgca aaatgagatc gatcgtctgc gtggcatcgc ggagactgcg
    27421 cagtgtggcg caattctcgg tatcggtggc ggaaaaaccc tcgatactgc caaagcactg
    27481 gcacatttca tgggtgttcc ggtagcgatc gcaccgacta tcgcctctac cgatgcaccg
    27541 tgcagcgcat tgtctgttat ctacaccgat gagggtgagt ttgaccgcta tctactgttg
    27601 ccaaataacc cgaatatggt cattgtcgac accaaaatcg tcgctggcgc tcctgcacgt
    27661 ctgttagcgg cgggtatcgg cgatgcgctg gcaacctggt ttgaagcacg cgcctgttcc
    27721 cgtagcggcg ctaccactat ggcgggcggc aagtgcaccc aggcggcgct ggcactggca
    27781 gaactgtgct acaacactct gctggaagaa ggcgaaaaag cgatgcttgc cgccgaacag
    27841 catgtagtaa cccctgcgct ggaacgcgtg attgaagcga acacctattt aagcggtgtt
    27901 ggttttgaaa gcggcggact ggcggcggct catgcggtgc ataacggcct gaccgctatc
    27961 ccggacgcgc atcactatta tcacggtgaa aaagtggcat tcggtacgct gacgcagctg
    28021 gtactggaaa acgcgccggt tgaggaaatc gaaaccgtag ctgcatttag ccatgcggta
    28081 ggtttgccaa taactctcgc tcaactggat attaaagaag atgtcccggc gaaaatgcga
    28141 attgtggcag aagcggcatg tgcagaaggt gaaaccatcc acaacatgcc tggcggcgcg
    28201 acgccagatc aggtttacgc cactctgctg gtagccgacc agtacggtca gcgtttcctg
    28261 caagagtggg aataacctac tccaaactcc cggcttgtcc gggagtttga acgcaaaatt
    28321 gcctgatgcg ctacgcttat caggcctacg caatctctgc aatatattga atttgcgtgc
    28381 tttttaggcc agataaggcg ttcacgccgc atccggcact ctgtgccagt aatctgaaac
    28441 tcctacgttg ttcaggagtt ttttatccgc ttctggacat tttctctaca gaacaatcgt
    28501 tagcccggaa ggtcgaatct gtgactctta ttttcacgga cgaaccagat gaaagcttca
    28561 ctggcgctgc tcagcctgct aacggcattt accagccact ccttaaaatc tcccgcagtt
    28621 ccaccaaccg tggtacaaat ccaggccaat accaaccttg ccatcgcaga cggtgccaga
    28681 cagcaaatcg gcaacacgct attttacgat ccggcgtatg tgcggcttac ctatcctggc
    28741 ggtgatgtcc cgcaagaacg cggtgtatgt tccgatgtgg tgatccgtgc attgcgcagc
    28801 cagaaggtcg atttgcagaa actggttcac gaagatatgg cgaagaattt tgccgagtac
    28861 ccgcaaaaat ggaagttaaa gcgcccggac agcaacatcg atcaccgtcg ggtgcctaat
    28921 ctggaaacct ggtttagccg ccacaacaaa acacgcccca tcagtaaaaa tgccggtgac
    28981 tatcaagcgg gcgatattgt ctcctggcga ttagataacg ggctggcgca tattggggtg
    29041 gtgtcagatg gcttcgcccg cgacggtacg ccactggtga tacataacat tggcgcaggc
    29101 gcgcaggaag aagatgtgct gtttagctgg cggatggtgg ggcattatcg ttattttgtt
    29161 aaataagcga tcgccccaca tgaagaaaaa attcagatgc tttttacact gacagcttct
    29221 tttttcttac ggctcaccag cgccagtgcc agcacaatca gcacaatacc gctaccttca
    29281 acggcccccg gattctcacc gagcagccac caggaaaaga gtacgccgca aacgggaacg
    29341 gccagcgtgc ttaagctggc aatactggca ggcaagtttt tcaatacaaa caaccataag
    29401 ctccacgcca gcgccgtcgc cagaatcgca ctgtaggcca gcgcccagaa cacggtgggc
    29461 tgccagtcaa tttcacgttg cggcaccagt aaagcgacca cactcatcac cagcgccgca
    29521 tagagcatct gccaggatgt taacgataat aaatccacgc gcggatgacg ggtgtatagg
    29581 cgtttagcaa caatcgcgct cgccccccaa ctaacaccgg agaggattgc cagcatggca
    29641 cttttcatcg aagagaaatc gagttgccac ggctgcaaca ccaaaaataa gccgaaagcg
    29701 gcaatcagaa tcgcgaaata ttgcccacgt cgcaggcgtt caccgagaaa caacgcggcg
    29761 aaaatcacca cccagaacgg catggtatag ctcaggatcg ccaccttccc cgcacctccg
    29821 ctgaccaacg cccactgcgc cagaccaacc atcccgcagg tttgtaacag ggcaatggct
    29881 aaggtgtatt taaacggcgt cgggtgcatt ccgcgaccac gtaataaaag gacgatgaat
    29941 aaaacgagag cgccgaaaat gcagcgtaag gcggtaaagt cgaaggcacc gatgtaactg
    30001 gtgacttgct tcatgaaaat ccagctataa ctccagataa gtgtgagcac aaccaggcca
    30061 ctgattgcca gtgggttgct ctttcctgcg gcagacatga gagatccagt gtgtagtttt
    30121 cctgtggaca ctatacgccg gtaaagaaac ttcagcgact gcctgaaaga cagtcactga
    30181 agaaggtcag attacagcag gtcgaaacgg tcgaggttca tcactttcac ccatgccgct
    30241 acgaagtctt taacaaactt ctcgtgggca tcgctgctgg cgtaaacttc cgctaccgca
    30301 cgcaggacag agttagaacc aaataccaga tccgcacggc tggctgtgta tttcacttcg
    30361 ccggtttcgc ggtcacgtcc ttcgaacagc tctttcgatt cgtcagtcgc tttccactcg
    30421 taacgcatat ccagcaagtt cacgaagaag tcattgctca atacgccaac gcggtcagtg
    30481 aagacgccgt ttttgctgct atcgaagttg gcacccagta cacgcatccc gcccaccagc
    30541 gcagtcattt ccggcgcggt cagcgtcagt tgctgtgctt tgtcgatcag cagtgactcg
    30601 gtggtggaaa cgtccagacg agcgcgatag ttacggaaac cgtcagcaat tggctccagc
    30661 agctcaaaca tctcaatgtc agtctgatcc tgacgcgcat caacgcgacc cggcgcaaac
    30721 ggtacatgaa tgctcaaacc tgcggcgctt gcggctttct caacaccaac cacaccagcc
    30781 agcacgatga tatccgccag cgaggcttta ccagactctt tctggatttt ctccagaaca
    30841 ggcagagcac gaacggctgc ggcgttcaca tcccagtcgc gctgcggcat taatgccaga
    30901 cgcgcaccgt tggcaccacc gcgtttgtcg ccaccacgga aggtagaagc agatgcccag
    30961 gccaccgata ccagctcact aacagacaga ccagaatccg caatcgcgaa tttcagatcg
    31021 ataatgtcct gctcggtcgg gttgtagatc ggctgcggca gcggatcttg ccagatcaga
    31081 tcttctttcg gcacttccgg cccgatgtag cgagatttcg gccccatatc cctgtgcgtc
    31141 agtttgaacc aggcacgggc aaaggcttcg ttgaacgcct gcggatcgtt gaggaaacga
    31201 cgagagatct tctcgaactc aggatcaaaa cgcagcgtca ggtcggtcac cagcattgtc
    31261 ggtttacgtt tcttcgacgg atcgaacgga tccgggataa tttccggtgc gtctaccgct
    31321 tcgaactgga ttgctccagc cggactgcgg gtctgtaccc actcatattt gaacaggttc
    31381 tcgaagaaat agttgctcca ctgggtcggc gtctgggtcc agacaacttc cagaccagag
    31441 gtaatggcat ctgcgccaac gccgctgccg taagtgctcg cccagcctaa accttgttct
    31501 tcaatcggtg cagcttctgg atcaggacct acatttgatg tcggaccggc accgtgagtt
    31561 ttacccagcg tatgaccacc cgcaatcagc gccacggttt cttcgtcgtt catgcccatg
    31621 ttgccgaagg tcgcacggat agctgctgcc gcagaaagcg gttcgccgct gtgatccggg
    31681 ccttccgggt taacgtaaat cagacccatc tcggttgcac ccagcggtgc tttcgccagc
    31741 gcttccggat gacggtgagt cagccaggct ttttcatcac cccagttaac atccagatcc
    31801 ggttcccaga cgtcttcacg accggcacca aaaccgaagg tacggaagcc ggagttttcc
    31861 agcgccacgt tacctgcgag gataaacagg tcggcccagg agattttctg accatatttc
    31921 tgtttgattg gccacaacag gcgacgcgct ttatcaaggc ttacgttatc cggccaggag
    31981 ttcagcggtg caaaacgttg ctgaccacga cccgcgccgc cgcgtccatc gattgaacgg
    32041 taagtacctg cgccgtgcca ggccatacga ataaacagac cggcgtagct gccccagtcg
    32101 gctggccacc acggctgaga ttctgtcagc agggctttca gatctttttt caggccgtag
    32161 taatctaatt tgctgaattc tttgcggtag tcaaagtcct cacccagcgg gttagaacga
    32221 ttagaatgtt ggtttaacag gtcaacacgg agttgatttg gccaccagtc gcgagtggtt
    32281 gtgcccgccc ccgcgctctg gtcgtgaccg ccctgatgga acgggcattt gccagtggct
    32341 gtcgtgttat gaatatcgtc tgacgtgctc atcaatgtgc tcccctctac agtgttaccg
    32401 ttacgataca cagcgttaga gagaagttat aattgaatta atccacggat gcgatagttg
    32461 agatcttaca tattgttggt taaagagatg taggtcaaat tgatcgtaat taagcttttt
    32521 tgattatgtc cgaattcgga caaatgcttt ataaaaaagg gttttagctt catatccttc
    32581 aagtaatgag ggagaataca aggctgacaa ccttattttc ccgccctcat ttcgaggcag
    32641 cattttgtgc tctgtttaaa atttgtgatc actgtgtgat tttcacaaaa gccacactat
    32701 ttataaacca ggtcgaacac ccagcgtatg gcaaatcgcg taactcattt cagcgcggtt
    32761 aagcgtatag aagtggaaat ctttcactcc ttcacggctt aaaatcttca ccatatccat
    32821 ggcaatattc gcgcccacca gtttgcgggt ttcggcatca tcatccaggc cgtcgaacat
    32881 ttgcgccatc cacgccggaa tacgcacgtt ggtcatatcg gcaaatttct tcgcctgttt
    32941 aaagttagat accggcaaaa ttcccggaat aatttccaca tcaatgcccg ccgatacaca
    33001 gcggtcacga aaacgcaggt agctttcgac atcgaagaag aactgagtaa tcgcgcggtt
    33061 ggctccggca tcgactttgc gtttcagatt aagcaaatcc gcctgagcgc tttttgcttc
    33121 cgggtgaact tccggatacg ccgccacgga gatatcgaaa tctgccactt cttttaatag
    33181 cgtcaccagg tcagaagcat acatttctgg cttaccactt cccggcggca gatcgccacg
    33241 cagcgccacg atatgacgaa taccgttatt ccagtagtcg cgtgcaatgg tgcgcaactc
    33301 gtcgggcgtc gcatcaatgc aagtaagatg cggtgccgct tccagaccag tgcgatcttt
    33361 aatgccttta ataatgctgt gcgtacggtc gcgctcgccg gagttcgcgc cataggtcac
    33421 cgatacaaac ttcggtttca ggctgctaag gcgatcgatg gagttccaca gggtctgctc
    33481 catttcactg gtacgcggcg ggaaaaactc gaacgaaacg ttaatctgcc cctggacttc
    33541 tgccaggcgc tgattcaggg catcccgctg gctggcgtga aaaaagctca taccttacct
    33601 catcaatcgc ttgtagttat atgttgtgtt gtgcacatct atccgtttag acgtccagat
    33661 gtaaagatga aggaaaagcc gaagggcgtc aaccgaaaaa tgaccaatga tggtgaggaa
    33721 aaatcaggga agatgaaaaa acttcaggat ggtaagaaaa agaaaatccg gtatgcaaag
    33781 caaaccggac tgcaaatata aactaaccat gtacttattg cttcttgccg gattcgtcgt
    33841 aaacgcttaa tccggcctaa tcctatgata attcaatata tttcattaga aaccataaac
    33901 ccgaaaacat gagtaccggg cattattaaa tttctgaaat tacaacaact gtgccagccg
    33961 gttgatatcc gactgaatcg ccccggcggt gacgtcgcgc cccgcgccag gcccacggat
    34021 caccagcggg ttgtcgcgat accagcggct ttcgatggca aagacgttat cgcacggcag
    34081 cagtgatgcc agcggatggt cttcacgcac cgcctccacg cccacacgcg ctttgccgtt
    34141 ggcatcgaaa cgcgccacgt agcgcagcac cagccccatt tcgcgggccg cttccagccg
    34201 ttgcaccatc tgctcgttaa gttcatcgcc attttcaaag aaatggtcga tgctgccgcc
    34261 ttcgcaatga gcaggcacca gcgattccac acgtacctgg tccggttcaa tgttgtaacc
    34321 cgcttcacgc gccagaatta ccagcttgcg cattacgtct ttgccggaga ggtcgtcacg
    34381 cggatcaggc tcggttaagc cctgctgcca cgcctgatcc accagctcgg taaacggcac
    34441 gctaccgtcg aattgcagga acagccagga gagcgtgccg gagaagatcc cgctgatcga
    34501 caaaatagta tcgccgctgt cgatcagatc gcgcacggtg tggttgatcg gcaagcccgc
    34561 accgacggtg gcattgtaca gccagtgacg cccggttttt tcgaaggcgt cgtggatctg
    34621 gcgatattta ttgctgtcgc tggctcccgc cagtttgttg gcgctgataa cgtggaaacc
    34681 gtggctggcg aaatcaagat actgatcagc aagctgctgg ctggcggtaa cgtccagcac
    34741 cactaaatca tcatacggat gggcgcgcat ccacaggaac aacgactctt catcctgctc
    34801 aaccgcttca tcgttgaaga aggctaacgc gcggctggcg tccagcccgt catagctcaa
    34861 caggctgcgg cggctgtcca ccacacctgc cagcacaaac tcaaagccgg tacgtgccga
    34921 aagcgtgctc tgctcacggg cgaacagttc cagccaacgg gaaccgatat tgcccttacc
    34981 gaacaatacc aggccgatgc gtttttctgc gcggaagacg gactgatgca gcccctgaat
    35041 caggctttcg gtcgggccgg tgcgcagtac tgccaccagg ctgatgccgt catcggactg
    35101 ccaggtaaat tcgaccggct ggcctttcag ttgctgccag aagcggtggc aatgcagcgg
    35161 gttacgggtg acgcctgcac cgaccatcgc caccagcgcc agcccctgac gcaggcgcag
    35221 ttcgccaggt aatcccgctt cgtcgaggat tttcagcgca ctgtcggcca cttctgaggt
    35281 gtagcaaaat tgcagcaact ggcgatcgtt atgtacgcca accgccagcg ggcgtacctg
    35341 cgcgcgtttc aggatttggt cgatctcttt atgcgccagt ttgaaatcct gactggcggg
    35401 cacctgaaac tcaatcaaac agacatcatc gtggctggtg acaatacgcg caccagtacc
    35461 ggaggccagc acgcgttcaa tgcgcgtgga accttgatcc ggcgtgtagc tacagcgcag
    35521 ttgcaggtcg atttcgctgc cagaaaccgg ctgtaaagta cgggcgtgaa gaacgggagc
    35581 cgccaggcgc gccagttcgc tggcctcatc cagacgcagc aacggcagca ggcaggcatc
    35641 tttcacttta cgcgggtcgg cactgtatac cccggcgacg tcgctccaga tggttacgcg
    35701 agaaacaccc gccagcgcac cgatttgtgt cgcggaatag tcggaaccgt tacgccccag
    35761 cagcaccgtt tcaccggcgt tgttgcggct gataaatccg gtcaccacca gacgtttgcc
    35821 cggatgttgc accagcagct gttgcagcaa cgggtaagaa agcccttcat caacctgcgg
    35881 ttgtgcggcg cgttcagcgc gtaaaaactc gcgggcatca agccaggccg ctggcagccc
    35941 ttgttgatta agtaccgcag acatcaggcg tgccgaccat acttccccgt ggcccaccac
    36001 ttccgcatac actgcgtcgt taataccgct gtcgagcagc gccgccaggc gctcaaggtc
    36061 gctgacaaaa gcgctaatga ggctatcagc ttcttcagcg ggtagcagac cgctaatcag
    36121 atcgcactga tagcgacgca gcgtttgttg aacctgatgc gcagagagac gatcggtctg
    36181 gcttagtttc aaccagttaa tcaactggtt agtggtgcta ccggcggcgg aaaccaccat
    36241 catatcgtca ggctgagagt actccgccat aatgcccgca acacgcaaat aacacttcac
    36301 atcagccaga ctactgccac caaatttatg cagttgacga cctttcgccc ctgcctgcgc
    36361 aatcacactc atttttaccc cttgtttgca gcccggaagc cattttccag gtcggcaatt
    36421 aaatcttcgc catcttcaat accggtggag atacgcagca gcgtctcgga gatcccggcg
    36481 gcagcacgcg cttctggtgc catgcctgca tgtgtcatgg ttgcggcgtg agagattaaa
    36541 ctttccactc cccctaatga ttccgccagc gtaaacaacg acagcccgcc caggaaacga
    36601 cgcagcgtct gctcatcgcc atccagttca aaactcaaca ttgcgccaaa gcctttttgc
    36661 tggcgcgcgg caatttcatg cccctgattt tccggcagcg acgggtgata cagttttttc
    36721 accaacggct gggtttgcag atatttcaca atcgcctgcg cgttgcgctg ggccagctcc
    36781 atacgcggca ccagcgttcg caacccgcgt agcagcagat agctgtcaaa cgcgccgccc
    36841 gtcacgccaa tattgtttgt ccaccaggca agttcagtga caacgtccgg gtctttagca
    36901 atcaccacgc cagccaccac gtcagagtga ccgttcagat atttcgtaca tgaatgcaac
    36961 accagatcgg cacccaatgc cagcggattt tgtaatgccg ggcttaagaa ggtgttatcc
    37021 accacgctca ccgccccgac ttcccttgcc agatggcaga ttttcgcaat atccacgacg
    37081 cgtaacaatg gattacttgg gctttctacc agtaccagtt taggtttttc tgccagcgct
    37141 gcccgtaatg cctgttcatc gccttgatca acaaacaaca cgcgatagca accgcgtttc
    37201 gccagactgt cgaacaggcg atagctaccg ccgtagcagt cgtgcggcgc aaccagcaga
    37261 tcgccaggtt tcaaaaagac ggtcgttacc aagtgaatcg cggacatgcc ggtattagta
    37321 agtactgcgc cagcaccgcc ttccagttct gccagcgcac gctgaaccac atcgcgcgtt
    37381 gggttgccgc gacgcgagta atcatgcgcg cgcggttcat taaatccggt aaagttatag
    37441 gtgctggaaa gatggatggg agggacaacg caaccatact gttcgtcgtc atttaacccg
    37501 ctacgcactg cgatggtggc ctgtttacgc gtcatgtgat gaagtttcct gggctttgtc
    37561 ggtgaaatgt caggcaccag agtaaacatt gtgttaatgg acgtcaatac atctggacat
    37621 ctaaacttct ttacgtatag attgagcaaa tcccaaatag ccgttaaaat tatatgcatt
    37681 atcacgccga caggtgcatt acacgatgtc acggtaacgc ctgtacggta aactatgcgg
    37741 gtttacggtc agtacccaca taaattgtgt ggtctggtct caatttattg acgaagagga
    37801 ttaagtatct catggctgaa tggagcggcg aatatatcag cccatacgct gagcacggca
    37861 agaagagtga acaagtcaaa aagattacgg tttccattcc tcttaaggtg ttaaaaatcc
    37921 tcaccgatga acgcacgcgt cgtcaggtga acaacctgcg tcacgctacc aacagcgagc
    37981 tgctgtgcga agcgtttctg catgccttta ccgggcaacc tttgccggat gatgccgatc
    38041 tgcgtaaaga gcgcagcgac gaaatcccgg aagcggcaaa agagatcatg cgtgagatgg
    38101 ggattaaccc ggagacgtgg gaatactaaa gcaaaaaaga gcggcgcgga gtggaatcgc
    38161 ctgatgcgct acgcttatca ggcctacgtc atattgcaat ttattgaatt tgcacgaact
    38221 tgtaggccgg ataaggcgtt cacgccgcat ccggcataaa caacgagcac gttgtctgcg
    38281 acccaccgct ttttatacat ggacgtttaa ctatgaaaaa caggctgctg atcctcagcc
    38341 tgctggtttc tgtacctgcc tttgcctggc agccacaaac cggcgacatc atctttcaga
    38401 tctctcgctc atcgcaaagt aaagcgatcc aactggcgac ccattccgat tatagccaca
    38461 ccggtatgct ggtgatacgc aacaaaaagc cctacgtttt tgaagcggtc ggcccggtga
    38521 aatacacccc gctcaagcag tggatcgccc atggtgaaaa gggcaaatac gttgttcgcc
    38581 gcgttgaagg cggactgagt gttgaacaac agcaaaaact ggcgcaaacg gtaaaacgtt
    38641 atctcggtaa accgtatgat ttcagcttct catggagcga cgatcgccag tactgttcgg
    38701 aagtggtgtg gaaggtttac cagaacgcgc tgggaatgcg cgtgggcgag cagcagaagt
    38761 tgaaagagtt cgatctcagc aacccgctgg tccaggctaa gctcaaagag cgctacggca
    38821 aaaatattcc gcttgaggaa acggtagttt caccacaagc cgttttcgac gcgccacaac
    38881 tcaccacggt tgccaaagag tggccgctgt tttcgtggta atggatgatg aaaaaaataa
    38941 ttcccgccgt agtctgtttg ttgctttgta gttgcacgac ctttccgcaa actgtcgctc
    39001 cggtaaacgg tgatgcctcc aattagttga attaaggata taatactcgc ttttaaggtt
    39061 gtttcatggt cacagttaac cttcattgcc cccgttgcca gtccgttcag gtttaccgtc
    39121 acgggcagaa cccaaaaaga catgatcgat tccgcggccg tgactgtcac cgggtatttc
    39181 agctcagtta ctgttacgag ccccgtaaac cgggagttaa agaccaaatc gctgaaatga
    39241 ctttcaacgg tgccggggtt cgcgatactg cccgtacgct gaaaatcggc attaatacag
    39301 tcatccgaac tttaaaaaac tctcgccacg aagaataacg tcatccccgg tcgctcatgc
    39361 tgatgtggca ctcatctgcg aacttagtca gcaatggagc tttgtcggca gtaaagcgcg
    39421 acagcactgg ctctggtacg tgtacaacac gaaaactggt ggtgttctgg cctacacttt
    39481 tggcccccgg actgacgaaa cctgtcgtga attgctggcg ctgcttaccc ttttaccatc
    39541 ggcatgttga ccactgacga atggggtagt tacaccagag agttaccgaa ggaaaagcat
    39601 ctgactggta agatttttac tcagcgcatt gagcgcaaca atctgaccct gagaacccga
    39661 atcaaacgtc tggcccgcag aaccatctgt ttctcgcgct ctgtagaact tcatgaaaaa
    39721 gtcatcggtg cttttatcga aaatatatgt tctactaatt ggagtcatca cccttctgat
    39781 gagctttcct tggatgatta ccagactgaa tacacagcca aagattttta cctcattctg
    39841 ctcttttttc tcaaatgaca tatattatat tttacatcat attcataggc atatgacatt
    39901 cccccgcccc cattatgtca ttgtgggttg atgttgggct ttcataaaaa aaacatcttt
    39961 ttttgaacta gcattaatta tatttacttt atatattaaa atattttctc catctggaaa
    40021 tattttagcc acacaatata aagcagctga atccgaatta cttaaaaata tattatcatt
    40081 acccataact ccagccatta aattactgga gactaaagca aggaaataac aacattgtat
    40141 tgccttcatt ttaattactc ccttgtctga taattgtaac tccattccct ttagctttgg
    40201 gattatattt tagataggtg ttatttggtg ttcccaagta actgcttgta ttcttttttt
    40261 cccattccat tcatataaga attcgttaaa tctttgcttg aaaaatttag actatcataa
    40321 acatcatttt cttttgttac tttatttcct ttatcgtcag agtaaaaacc gtgagtgtga
    40381 taatcgccta ctctctctga accttcaggg cattttatat ttcgcaagtc tacactatca
    40441 ttatcatttg aacttatcgg attcatgggg aaatattcac cttgcttctt acatattagt
    40501 ccaccatatt ctttattctc gttaattgat tctccattac acat
//
LOCUS       DADLFA010000011        40425 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000011 DADLFA010000000
VERSION     DADLFA010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40425
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.011"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(115..2436)
                     /locus_tag="JQH65_000518"
     CDS             complement(115..2436)
                     /locus_tag="JQH65_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAY6138026.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFITRYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(2533..3045)
                     /gene="greB"
                     /locus_tag="JQH65_000519"
     CDS             complement(2533..3045)
                     /gene="greB"
                     /locus_tag="JQH65_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709179.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HAY6138027.1"
                     /translation="MRIIKQTKGINEMKTPLVTREGYEKLKQELNYLWREERPEVTKK
                     VTWAASLGDRSENADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAW
                     VEIENDDGVTHRFRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASW
                     YVNAIEYVKP"
     gene            3237..3956
                     /gene="ompR"
                     /locus_tag="JQH65_000520"
     CDS             3237..3956
                     /gene="ompR"
                     /locus_tag="JQH65_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HAY6138028.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            3953..5305
                     /gene="envZ"
                     /locus_tag="JQH65_000521"
     CDS             3953..5305
                     /gene="envZ"
                     /locus_tag="JQH65_000521"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HAY6138029.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(5381..7003)
                     /gene="pckA"
                     /locus_tag="JQH65_000522"
     CDS             complement(5381..7003)
                     /gene="pckA"
                     /locus_tag="JQH65_000522"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAY6138030.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            7382..9106
                     /locus_tag="JQH65_000523"
     CDS             7382..9106
                     /locus_tag="JQH65_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HAY6138031.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNCSSRYSYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWGELFKLVGIKFFNTLFFATDWFMYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDVTFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            complement(9241..10119)
                     /gene="hslO"
                     /locus_tag="JQH65_000524"
     CDS             complement(9241..10119)
                     /gene="hslO"
                     /locus_tag="JQH65_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAY6138032.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSREHCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(10144..10545)
                     /gene="hslR"
                     /locus_tag="JQH65_000525"
     CDS             complement(10144..10545)
                     /gene="hslR"
                     /locus_tag="JQH65_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HAY6138033.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(10556..11224)
                     /gene="yrfG"
                     /locus_tag="JQH65_000526"
     CDS             complement(10556..11224)
                     /gene="yrfG"
                     /locus_tag="JQH65_000526"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HAY6138034.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(11289..13424)
                     /gene="igaA"
                     /locus_tag="JQH65_000527"
     CDS             complement(11289..13424)
                     /gene="igaA"
                     /locus_tag="JQH65_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HAY6138035.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRSQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLDQQTDIDIYLDCHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            13744..14304
                     /gene="nudE"
                     /locus_tag="JQH65_000528"
     CDS             13744..14304
                     /gene="nudE"
                     /locus_tag="JQH65_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HAY6138036.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(14469..17021)
                     /gene="mrcA"
                     /locus_tag="JQH65_000529"
     CDS             complement(14469..17021)
                     /gene="mrcA"
                     /locus_tag="JQH65_000529"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HAY6138037.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            17141..17920
                     /gene="hofM"
                     /locus_tag="JQH65_000530"
     CDS             17141..17920
                     /gene="hofM"
                     /locus_tag="JQH65_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HAY6138038.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALFVDPDS
                     VAICGEGGFDPWEAVSVRQPLLPPPGGDFAIALGLALGKAY"
     gene            17920..18459
                     /locus_tag="JQH65_000531"
     CDS             17920..18459
                     /locus_tag="JQH65_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HAY6138039.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLRKQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            18443..18883
                     /gene="hofO"
                     /locus_tag="JQH65_000532"
     CDS             18443..18883
                     /gene="hofO"
                     /locus_tag="JQH65_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HAY6138040.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            18873..19277
                     /gene="hofP"
                     /locus_tag="JQH65_000533"
     CDS             18873..19277
                     /gene="hofP"
                     /locus_tag="JQH65_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HAY6138041.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            19189..20427
                     /gene="hofQ"
                     /locus_tag="JQH65_000534"
     CDS             19189..20427
                     /gene="hofQ"
                     /locus_tag="JQH65_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HAY6138042.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGIEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEYERRELVVFITPRLVSSE"
     gene            20828..21349
                     /gene="aroK"
                     /locus_tag="JQH65_000535"
     CDS             20828..21349
                     /gene="aroK"
                     /locus_tag="JQH65_000535"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HAY6138043.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            21406..22494
                     /gene="aroB"
                     /locus_tag="JQH65_000536"
     CDS             21406..22494
                     /gene="aroB"
                     /locus_tag="JQH65_000536"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAY6138044.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            22586..23872
                     /gene="damX"
                     /locus_tag="JQH65_000537"
     CDS             22586..23872
                     /gene="damX"
                     /locus_tag="JQH65_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HAY6138045.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGDKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            23979..24815
                     /gene="dam"
                     /locus_tag="JQH65_000538"
     CDS             23979..24815
                     /gene="dam"
                     /locus_tag="JQH65_000538"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HAY6138046.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            24833..25510
                     /gene="rpe"
                     /locus_tag="JQH65_000539"
     CDS             24833..25510
                     /gene="rpe"
                     /locus_tag="JQH65_000539"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAY6138047.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            25503..26261
                     /gene="gph"
                     /locus_tag="JQH65_000540"
     CDS             25503..26261
                     /gene="gph"
                     /locus_tag="JQH65_000540"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HAY6138048.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALAWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     SEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            26254..27258
                     /gene="trpS"
                     /locus_tag="JQH65_000541"
     CDS             26254..27258
                     /gene="trpS"
                     /locus_tag="JQH65_000541"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAY6138049.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            27527..28432
                     /locus_tag="JQH65_000542"
     CDS             27527..28432
                     /locus_tag="JQH65_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6138050.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            28449..28811
                     /locus_tag="JQH65_000543"
     CDS             28449..28811
                     /locus_tag="JQH65_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAY6138051.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            28895..30058
                     /locus_tag="JQH65_000544"
     CDS             28895..30058
                     /locus_tag="JQH65_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HAY6138052.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLSVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRVQFVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLIQAR
                     DQLAKSGIALEQLNAPSATSCTSLPLLAQYGVTHAEPGHALTGTIPANQQGDQSERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     SLAGEFPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGRYDSLGNSLGA"
     gene            30058..31284
                     /locus_tag="JQH65_000545"
     CDS             30058..31284
                     /locus_tag="JQH65_000545"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAY6138053.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFCDVIDRVEQALVSAGWQVERRGDELQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDKKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLVDAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPLLVYQQGLAATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            31281..32159
                     /locus_tag="JQH65_000546"
     CDS             31281..32159
                     /locus_tag="JQH65_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HAY6138054.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLGVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGLIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYL
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            32170..32523
                     /locus_tag="JQH65_000547"
     CDS             32170..32523
                     /locus_tag="JQH65_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HAY6138055.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            32535..33839
                     /locus_tag="JQH65_000548"
     CDS             32535..33839
                     /locus_tag="JQH65_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6138056.1"
                     /translation="MDLYIQIIVVACLTGMTSFLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            33851..34936
                     /locus_tag="JQH65_000549"
     CDS             33851..34936
                     /locus_tag="JQH65_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAY6138057.1"
                     /translation="MKTFPLQSLTLIEAQQKQFALVDTICRHFPGAEFLTSGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRCLVFAPVMHAVQAGVSERLLALLNGGEVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(35088..35819)
                     /gene="phoB"
                     /locus_tag="JQH65_000550"
     CDS             complement(35088..35819)
                     /gene="phoB"
                     /locus_tag="JQH65_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HAY6138058.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCKKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(35919..36704)
                     /gene="frlD"
                     /locus_tag="JQH65_000551"
     CDS             complement(35919..36704)
                     /gene="frlD"
                     /locus_tag="JQH65_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAY6138059.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDETLRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(36701..37531)
                     /gene="frlC"
                     /locus_tag="JQH65_000552"
     CDS             complement(36701..37531)
                     /gene="frlC"
                     /locus_tag="JQH65_000552"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HAY6138060.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELYEYAENIGMDLILEPLTPYESNIVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIERGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            complement(37581..38603)
                     /gene="frlB"
                     /locus_tag="JQH65_000553"
     CDS             complement(37581..38603)
                     /gene="frlB"
                     /locus_tag="JQH65_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HAY6138061.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQAAKHLADRFSDLQVYVISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(38624..40012)
                     /gene="frlA"
                     /locus_tag="JQH65_000554"
     CDS             complement(38624..40012)
                     /gene="frlA"
                     /locus_tag="JQH65_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HAY6138062.1"
                     /translation="MTANSPLQRVGQEKGIAMGSQELQRKLGFWAVLAIAVGTTVGSG
                     IFVSVGEVAKAAGTPWLTVLTFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGS
                     RPLAFLSGWASFWANDAPSLSIMALAIVSNLGFLTPIDPLPGKFIAAGLIIAFMLLHL
                     RSVEGGAAFQTLITIAKIIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGI
                     SATSWSYTGMASICYMTGEIKNPGKTMPRALIGSCLLVLVLYTLLALVISDLMPFDKL
                     ANSETPISDALTWIPALGSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFF
                     KCFGHVHPKYNTPDVSIILQGALGLFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWC
                     RKRDDYKPLWRTPAFGLMTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAF
                     WAKRSRQLNALS"
     gene            complement(40257..40424)
                     /locus_tag="JQH65_000555"
     CDS             complement(40257..40424)
                     /locus_tag="JQH65_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HAY6138063.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
BASE COUNT         9347 a        10254 c        11118 g         9706 t
ORIGIN      
        1 tgtggaataa aaaggccgga gatattctcc ggcactttta tgtcaggcaa ggcgttcgtt
       61 gccagatgcg gcgtgaacgc cttatcgcgc aggtttaggg gccagtccgg gcgtttaacg
      121 ttttttgccc attgccgccg ccagcgcatc catcatcgcg ctattaccgg caggctgcgc
      181 ttcacgaccg cgtggtttgg ctgccgggcg gttgttttgc gggcgttcat taccgccgcc
      241 gcgacgggcg ttggtttcgc caggctgctc gtccagacgc atagtcaggg cgatacgttt
      301 acgctgaaga tccacttcca gcactttcac cttcacaatg tcgcccgctt tcaccacggt
      361 atgcggatct tccacaaact tgttcgacaa tgaagagatg tgaaccaggc cgtcctgatg
      421 cacgccgata tcgacaaacg cgccaaagtt ggtgacgttg gtcaccgcac cttcgaggat
      481 catacccggt tgcaggtcgt tcattgtctc gacgccatcg gcaaactgag cggttttaaa
      541 ttccggacgc ggatcgcgac ccggtttttc cagctctttg atgatgtcag ttactgtcgg
      601 cacaccgaat ttttcatcag taaagtcaga cgctttcagg ttacgcagtt cgctgctgtt
      661 acccatcaga tctttcagtg cctgctgtgt tgctgccaga atgcgttcca ccaccggata
      721 ggcttccggg tggacggtag aagcatccag cgggttatca ccgtggttaa tgcgcaagaa
      781 gcccgcgcac tgctcgaagg ctttcggccc cagacggctg actttcagca gttgctgacg
      841 gttctggaac tggccgttct catcgcgcca ggccacgatg ttttgcgcca tcatgcgcgt
      901 caggcccgcc acgcgggtta acagcggaac agaagcagtg ttgagatcga cgccaacggc
      961 gtttacgcag tcttctacta ctgcgtccag tttgcgggcc agttgcgtct ggctgacgtc
     1021 atgctgatac tgacctacgc cgatagattt cggatcgatt ttcaccagct ccgccagcgg
     1081 atcctgcaaa cggcgggcga tagacaccgc gccacgcagc gaaacgtcga gatccgggaa
     1141 ctcctgcgct gccagctcgg aagctgagta aaccgacgcg ccagcttcgc tgacgatcac
     1201 tttctgtgcg gtcactttcg ggaactgctt ctgcacgtcg agatagaaac gttcagtttc
     1261 gcgggaagct gtaccgttgc cgatcgccac cagttcaacg ttatgttttt cacacaaggc
     1321 agcaacggtc atcgctgctt ttgcggcttg tccggtgtgc gggtaaatgg tatcggtcgc
     1381 taccagtttg ccagtggcat cgaccaccgc cacttttacc ccagtacgca gacccggatc
     1441 gaggcccatc gttgcacgca gtccggcagg ggccgccatc agcagatcgt gcaggttacg
     1501 ggcaaagacg ttgattgctt catcttccgc gcgttcgcgc acggtaccca tcagttcggt
     1561 ttccagatgc atcagcacct tgatgcgcca ggtccagctc actacgcctt tgcgccagct
     1621 atccgccggg gcattgttca ggcgcaggcc aaggtgatcc atgatgattt gctcgcaata
     1681 gctctctttg ggcggctcat cgaactgcgg atcggcattc agcgaaagct ggagtacgcc
     1741 ttcgttacgc ccacggaaca tcgccagagc gcggtgagaa ggcaccgtgg acaacggttc
     1801 gtgatgatcg aaatagtcgc ggaatttcgc cccttcctct tctttaccgc tcaccaccgt
     1861 agaaaccaaa tgcgcgttct tccacagata atcacgcact ttcgccagca gcgcggcatc
     1921 ttcggcaaac cgttccatca ggatatagcg cgcgccatcc agcgcggctt tggtatctgc
     1981 cacgccttta tcggcatcaa catattgtgc agcggcgact tctggcgtgt gtgacggatc
     2041 gctccacagc aggtcagcca acggctcaag ccctgcttca atggcgattt gcccgcgggt
     2101 gcggcgttta ggtttgtagg gcaggtagag gtcttcgagt tcggttttgc ttagggtggc
     2161 gttgatggcc ttcgccagat catcggtgag tttgccttgc tcggaaatgg acttgaggat
     2221 cgcctgacgt ctctcttcca gctcgcgcag atagctcaga cgcgtttcca gattacgcag
     2281 ctgcgtgtca tccagaccgc cggtgatttc cttacgataa cgtgtgataa acggcacggt
     2341 attcccttcg tcaagcaggc gaacggcagc gtcaacctgt tccgggcgcg cctgaatttc
     2401 acccgcaata atgcggcaga acgaatcatt catcatggtt tgagttcatc ttttcggatc
     2461 aaaaatcagg ggatagttat acggactggc tggcaaaaat gccagccatc ggcaggaggt
     2521 taagactctt ccttacggtt tcacgtactc gatagcatta acataccagc tcgcttcccc
     2581 ggcaggggta ttcaccaccg ccagatcgcc gacttctttt ttcagcaatg cgcgggccat
     2641 cggggaatcg atagagatgt aatctttacg gccaaaaatt tcatcgtagc cgacaatacg
     2701 gaaacggtga gtcacgccat cgtcgttttc aatctccacc cacgcgccaa aaaagacttt
     2761 gccttcctgc tgaggggagt aatcgacgat tttgagattt tccatgcatt tagtgagata
     2821 gcgcacgcga cggtcgattt cacgcagacg ctttttatta tactgatagt cagcattttc
     2881 gctgcggtcg cccagacttg cggcccaggt cacctttttt gtgacctccg ggcgttcttc
     2941 acgccagaga taattaagct cttgtttgag tttttcatac ccttcccggg taaccagggg
     3001 cgttttcatc tcgttgattc cctttgtctg tttgataatg cgcacattgg gtataacgtg
     3061 atcatatcaa cagaatcaat aatgtttcgc cgaataaatt gtatacttaa gctgctgttt
     3121 aatatgcttt gtaacaattt aggctgaaat tcataccaga tttagctggt gacgaacgtg
     3181 agctttttta agaatacacg cttacaaatt gttgcgaacc tttgggagta caaacaatgc
     3241 aagagaacta caagattctg gtggtcgatg acgacatgcg cctgcgtgcg ctgctggaac
     3301 gttatctcac cgaacaaggc ttccaggttc gaagcgtcgc taatgcagaa cagatggatc
     3361 gcctgctgac tcgtgaatct ttccatctta tggtactgga tttaatgtta cctggtgaag
     3421 atggcttgtc gatttgccga cgtcttcgta gtcagagcaa cccgatgccg atcattatgg
     3481 tgacggcgaa aggggaagaa gtggaccgta tcgtaggcct ggagattggc gctgacgact
     3541 acattccaaa accgtttaac ccgcgtgaac tgctggcccg tattcgtgcg gtgctgcgtc
     3601 gtcaggcgaa cgaactgcca ggcgcaccgt cacaggaaga ggcggtaatt gctttcggta
     3661 agttcaaact taacctcggt acgcgcgaaa tgttccgcga agatgagccg atgccgctca
     3721 ccagcggtga gtttgcggta ctgaaggcac tggtcagcca tccgcgtgag ccgctctccc
     3781 gcgataagct gatgaacctt gcccgtggtc gtgaatattc cgcaatggaa cgctccatcg
     3841 acgtgcagat ttcgcgtctg cgccgcatgg tagaagaaga tccagcgcat ccgcgttaca
     3901 ttcagaccgt ttggggcctg ggctacgtct ttgtaccgga cggctctaaa gcatgaggcg
     3961 attgcgcttc tcgccacgaa gttcatttgc ccgtacgtta ttgctcatcg tcaccttgct
     4021 gttcgccagc ctggtgacga cttatctggt ggtgctgaac ttcgcgattt tgccgagcct
     4081 ccagcagttt aataaagtcc tcgcgtacga agtgcgtatg ttgatgaccg acaaactgca
     4141 actggaggac ggcacgcagt tggttgtgcc tcccgctttc cgtcgggaga tctaccgtga
     4201 gctggggatc tctctctact ccaacgaggc tgccgaagag gcaggtctgc gttgggcgca
     4261 acactatgaa ttcttaagcc atcagatggc acagcaactg ggcggcccga cggaagtgcg
     4321 cgttgaggtc aacaaaagtt cgcctgtcgt ctggctgaaa acctggctgt cgcccaatat
     4381 ctgggtacgc gtgccgctga ccgaaattca tcagggcgat ttctctccgc tgttccgcta
     4441 tacgctggcg attatgctat tggcgatagg cggggcgtgg ctgtttattc gtatccagaa
     4501 ccgaccgttg gtcgatctcg aacatgctgc tttgcaggtc ggtaaaggga ttattccacc
     4561 gccgctgcgt gaatatggcg cgtccgaggt acgttcggtt acccgcgcct ttaaccatat
     4621 ggcggctggt gttaagcaac tggcggatga ccgcacgctg ctgatggcgg gggtaagtca
     4681 tgacttacgc acgccgctga cgcgtattcg cctggcgacc gagatgatga gcgaacagga
     4741 tggctacctg gccgaatcga tcaataaaga tatcgaagag tgcaacgcca tcattgagca
     4801 gtttatcgac tacctgcgca ccgggcagga gatgccgatg gaaatggcgg atctcaacgc
     4861 agtactcggt gaggttattg ctgccgaaag tggctatgag cgggaaattg aaaccgcgct
     4921 ttaccccggc agcattgaag tgaaaatgca cccgctgtcg atcaaacgcg cggtggcgaa
     4981 tatggtggtc aacgccgccc gttacggcaa tggctggatc aaagtcagca gcgggacgga
     5041 gccgaatcgc gcctggttcc aggtggaaga tgacggtccg ggaattgcgc cggaacaacg
     5101 taagcacctg ttccagccgt ttgtccgcgg cgacagtgca cgcaccatta gcggcacggg
     5161 attagggctg gcaattgtgc agcgtatcgt ggataaccat aacgggatgc tggagcttgg
     5221 caccagcgag cggggcgggc tttccattcg cgcctggctg ccagtgccgg taacgcgggc
     5281 gcagggcacg acaaaagaag ggtaaataaa cgggaggcga aggtgcctcc cgttttgctt
     5341 tctataagat actggataga tattctccag cttcaaatca ttacagtttc ggaccagccg
     5401 ctaccagcgc ggcacccgca ggggtgtcgg tgtatttatc gaagttgtcg ataaacagtt
     5461 tcgccagggt ttcggctttt tcctgccact gttccggaga agcgtaggtg ttacgcggat
     5521 cgagaatctt cgtgtctacg cccggcagtt cggttgggat cgccaggtta aacatcggca
     5581 gagtgaaggt ttctgcatta tccagcgaac cgttgaggat ggcgtcgata atggcgcggg
     5641 tatctttaat cgagatacgt ttgcctgtgc cgttccagcc agtgttaacc agataagcct
     5701 gcgcgcccgc cgcctgcata cgtttcacca gcacttctgc gtactgagtc gggtgcagcg
     5761 acaggaatgc cgcgccgaag caagcggaga aggttggcgt cggttcggtg atgccacgct
     5821 cagtaccggc cagtttggcg gtgaagccag agaggaagtg atactgggtt tgatcggcag
     5881 tcaggcgaga aaccggcggc aacacgccga aagcatcagc agtcaggaag ataaccttag
     5941 tcgcgtggcc cgctttggaa accggcttaa cgatgttatc gatgtgatag attggataag
     6001 aaacgcgggt gttctcggtt tttgaaccat catcaaagtc gatagtgcca tcttcacgca
     6061 cggtgacgtt ttccagcagc gcatcacgac ggatagcgtt gtagatttca ggttccgctt
     6121 ctttcgacag cttgatagtt tttgcgtagc agccgccttc gaaattaaac acgccgtcat
     6181 cgtcccagcc gtgttcgtca tcgccaatca ggcggcgttt cgggtcggtg gaaagggtgg
     6241 ttttaccggt gccggaaagg ccgaagaaca ccgcaacatc gcctttctcg ccaacgttgg
     6301 cggagcagtg catagaagcg atacctttca gcggcagcag gtagttcatc atcgagaaca
     6361 tccctttctt catttcgccg ccgtaccagg tgccgccaat cagctgcatg cgctcggtca
     6421 ggttaaacgc cacgaagttt tcggagttca gaccctgttc tttccactgc gggttagtgc
     6481 acttcgcgcc gttcataacg ataaagtctg gtttgaaacc tgccagctct tcatcgctcg
     6541 ggcgaataaa catgtttttg acaaaatgcg cctgccaggc cacttcggtg atgaaacgga
     6601 cggaaagacg agtatccggg ttcgcaccac agaaagcgtc gacaacgaac agacgtttgc
     6661 cggaaagctg cctggtcacc aggcctttca gatgctgcca ggtttccgga gagagaggtt
     6721 tgttgtcgtt cttacctttg cctttgtctg cccaccagaa agtatcgcga gtggtatcgt
     6781 cacggacgat atacttatct tttggtgaac gaccggtgaa gatcccggta tcgacggcaa
     6841 cggcacccag attagttaac accccgcgct cataacctgt caggctcgga tcgagctctt
     6901 cctgatacag caggtcgtag cttgggttgt aaacgatatc atgtacgtca ctgataccat
     6961 aagcctcgag ttcttgcggg gtcaaaccat tgttaacgcg catttcactg ctccttagcc
     7021 aatatgtatt gcctgaatag taaagtcttt ttgggggtgt taaccgcgac aaggctcata
     7081 gatttacgta tctggagaaa ttcatatttg acaaaaaacc ccgtgattcc tgttacgaaa
     7141 cggttgctat tatcgcagaa atagcatttt aggtgtggaa aatgttctca aaaggttaaa
     7201 tcttggttta tatggcggga atattgtgag tggaatcgca ttccttaatg aaattgatgc
     7261 aaattcaatc ggtaaagtat cgattcacca cgctaacttt ttttgattat tttctgaaaa
     7321 aagcaaaata ctactgcccg gatgcgataa ttaacccacc ttttgattct ggataaccaa
     7381 catggataac gttgaacttt caccggcaac acgctggggg atgattgcta ccggattact
     7441 tcaggggctg gtttgctacc tgttgatcgc ctggcttgcc ggaaaaaatc acagttggat
     7501 tgtttatggc gtgcctgcga ccgttgcgtt ttcatccgtt ttactcttct ccgtgatctc
     7561 ttttaaacag aagcgccttt ggggatggct ggcgctggtg tttattgcca cgacagggat
     7621 gagcggttgg ctgaagtggc aaactgatgg catgaacccc tggagagctg aaaaggcgat
     7681 ttgggatttt ggctgctatc tgctgttgat ggcaatgttg ttgctaccgt ggatacaaca
     7741 aagccttcgt atccgcaatt gcagtagccg ctatagctac ttttaccagt cagtatggca
     7801 taacgtactt atattactgg tgatttttct cgccaatggc ctgacgtggc tggtgctttt
     7861 actgtggggt gagttgttta aacttgttgg catcaagttt ttcaatacgc tcttttttgc
     7921 aaccgactgg tttatgtatc ttacattagg tctggttacc gcactggcgg tgattctcgc
     7981 gcgcacacag tcacgtttaa tcgactctat tcaaaagttg ttcacgttaa tcgccacggg
     8041 gttgctgccg ttagtatcat tgctaaccct gatgtttatc atcactctgc cgtttacggg
     8101 cctgagcgcg atttctcgcc acatctccgc cgccgggttg ctgttaacgc tggccttttt
     8161 gcaattgatc ttaatggcta ttgtccgcga tccgcaaaaa gcgtcacttc cctggacggg
     8221 gccgttgcgt tgcctgatta aaaccgcttt gctggttgct ccgctgtatg tgttcgtcgc
     8281 cgcctgggcg ttatggctgc gggtcgctca gtacggctgg actgtcgacc gcttgcaggg
     8341 cgcgctggcg gtgctggtgt tactggtgtg gtcgctgggg tattttgtca gtatcgtctg
     8401 gcgtaatggg caaaatcccc ttgttcttca gggcaaagtg aaccttgcgg tttcgttatt
     8461 ggtgttggtg atactggtgc ttcttaattc gccggtgctg gacagtatgc gtattagcgt
     8521 gaacagtcat atggcgcgtt atcagagcgg caaaaacacg ccagaccagg taactatcta
     8581 catgctcgaa cagagcggtc gctatggacg tgcggcgctt gagtcgctga aaagcgatgc
     8641 cgggtttatg aaagacccga aacgcgcgcg ggatctgctg atggcgttag atggagagca
     8701 acatcttcag gaacaggtat cggaaaaagt attagccgaa aatgtgttaa ttgcccctgg
     8761 ttctgttaaa ccggatgtga cattctggtc ggccttaatc caggatcgct ataacgtgat
     8821 gacctgtatt gaaaaagacg cctgcgtcct ggtcgagcaa gatctgaata gtgatggtca
     8881 ggcggagcgg atcctgtttg cttttaatga tgacagagtc attgtctatg gctttgactc
     8941 agacagaaaa gaatgggacg cgcttgatat gagtttactt ccgaacgaaa taacgaaaga
     9001 aaaattactc acagctgcga aggatggaaa actggggacg aggccgaaag cgtggcgcga
     9061 tctgacggta gatggtgaaa cgctggaaat aaacttaagt aagtgacaac gcttttatcg
     9121 gatacgacgc tgtatctgac caaaataatt tgcgatttat ttcataggcg ctggtaagcg
     9181 cagcacatca ggccattaag ttgtaaaatc cggcagagcc ctctctgccg gacatactca
     9241 ttaatgaact tgcggatctg ccggagacgc gttgttgcgg atttcagcaa tatccatcgc
     9301 attgaacaga tagtggttac cgcagtaatc acaatgcatg tcaatttcgc catcttccgc
     9361 caggatgcta tcaacttctt catcaggcag cgttttcagc gcatcggcgc aatgttcacg
     9421 cgagcaggtg catttgaact ccacatcctg cggatcgtaa accgtcacct cttcttcgtg
     9481 atacaaacgc cacaacactt cgtttgccgg taaggtcagc agttcttcgg ttttgatggt
     9541 ttcggttagc gtcgccaggt ggtcaaagtc gtcctgctgg gcattttgcg caggcattac
     9601 ctgcaacaac ataccgcctg cagccggttt gccgtctacg tcgccggtgc gaataaacag
     9661 gcgcgtcggc agctgttcag aacgcataaa gtaatcttcc aggcaggccg ccagggtatc
     9721 accttccaga ccaactacgc cctgatagcg ttcgccttcg ctcggggtaa tggtgatcac
     9781 cacgtaacca ttgccgacca gcgttttcag gtcggcattt tctggaattt cgccctgcac
     9841 gcgcgccaca ccgcgcattt gctggttatt gttaccgtta ataaccgcca gattcatcgg
     9901 accgtcgccc tgcagctgta cggtgatatc accatcaaac ttcagcgtag cggttaacag
     9961 gctggtcgca accagcagtt ctgccagcac gtttttaacg ggctgcggat aatcgtggtt
    10021 ctcaaggatc tgttgcaggg tttccgaaac ggttaccagt tcgccgcgaa cggcaaaatt
    10081 ttcaaacaga tagcgatgta attggtcatg ttgcggcata atcatctctc ttgcaggtga
    10141 cagttattca ctgtcgccgt gtttaaatcg taacaggtcg cggcgctctt ttttgtccgg
    10201 gcgtcggtcc gggtgcggca tggttaaggc attaagttta cgtgccatcg ccattttttc
    10261 gcgtttctct acactttccg cagtctcttc atacagcaag gctgcctcgc tggcggggcg
    10321 acgctgttca gtaatcgcct ttacaatcac cgtgcgttcg tcatttccct ggcgcagagt
    10381 gagcgtggca ttcagctcga cgattttgct cggcttgctg cgctgcccgt tgtaatgcac
    10441 cttaccgcct tcaatcattt cacgggccag cgcgcgggtt ttataaaaac gggcagccca
    10501 tagccattta tccagtcgaa cctcaacagc aggtttctct ttcatggcgt ctccttcaca
    10561 ttagcgaggg gatcaggcgg cggtagtcat tcagtgacgg atggcgttga tactgtttct
    10621 cggcaatccc ggaatcagga ttagtcacgc cgaggcagta acgaatacca aattgcgcgg
    10681 cagcatcgag aatcgcttcg ctgtcatcaa taaacagcgt tctttcagct ttcagacccg
    10741 tagcttcggc caccgcatgc cataaccgct gatcctcttt cggataacca aatgtgtggg
    10801 tggaaagtaa taaatcaagg tgtgcgtcca gacccgtatg ctcaagtttt accgccaggt
    10861 tgtgcggatg agcattggtg agcaaaattc gctgcttacc gctggctttc agtgcctcaa
    10921 gaaacggaat ggtatcttca cgcagtacgg cgcgcggtcc catctcggtg gtcattgcac
    10981 agatatccag acccagttgc tcactccagt aatcaagaca gtaccagttt agcgtatgct
    11041 gtacgtcgtg atattgctgg cgcatatatt ccatcgcttc ctgtggcgta accccgtttt
    11101 tcgcgcccca tgtttcaggc accagctttt gccagaaata gttatcgaag gcgaggtcga
    11161 gcaacgtgcc gtccatatcc agcagaacgg tatctacgtc ctgccaggca atgttgatat
    11221 gcatgaggga aatctccaga gtgaagcaat ttgcgcgaca gggtagcata acctgtcgcg
    11281 caaacgtgtt attcgataag gctttctgaa ggggtgatca gttgcgggtt caggcagctt
    11341 tcataatagg cctgaatctc catcatgcga gtgcgatgac gctggtaacg acgccaggcc
    11401 tgtacgccat tgtaaatggc gctacccagc atcgtcagta gcagcaatgt ggtgctgaga
    11461 tagcgccaca ggccggaacg atccgggatc ggatgaaggc caatatgctg cgtaccattg
    11521 gcgtcagtga agatttttgt gacgatacct tcggcgttaa acggcgtatg catcagcatt
    11581 tgtgccagtt tctggaaagc gttccactgt tcttgcggcg ggtagtcgta aagtgatgcc
    11641 gaaggccagg gctgatcaac aaaatcgctg ccttcgtcgc tgacaatcag gaatccgccg
    11701 ggggccggac tgtttagtga ttgtgccgct cgcgccgttt catgcgtgat aaacggcgcg
    11761 gtggaggttg ccaccaggtt atccagcgat tccgcactca ccgggcgtaa taacacattc
    11821 acgccatcga gcttcccggc gttggcgcgt tttaccagcg cgtcccagtc tttactgttg
    11881 ccgagattga ccagcgcatt tttcagtcgc acacagtcat ctttggcaga acataaatcc
    11941 gctgtcttca gtacaatgtc gccaaaatca tcaagcaata ccatgccgga tttttgaatc
    12001 gctgaacgta atgaggcact gacgcgagat tcatcttccg gtttagggtg cagctggcga
    12061 ttaactgctt cagtcaatgc cgtcgctttg ttgaccagtt cagattctgg taatggtaat
    12121 gagcgggcgt cattccagat gatctgcgag cagtcaaacg gtaaaaaagg tgaattggtt
    12181 ttcgcgctcc aggttccgga agtgcgaata ttacacattc ccgtaccgct aatacgcaat
    12241 gtatcgccta cccgcacgcc agcgtcagcc agttgtttta cgctggtggc ttcaatggtc
    12301 tgcgcgcctt tcatccatga gagagtgaat ttcagcggca tatccagcgg gatccagaat
    12361 aacagcataa acagcaccag cagcgagccc gcagcgataa tcgtactgcg cagccagtgc
    12421 tgtaacggaa agttttttac ttcatcatgc agcgaaagat atcgcccctg acgcactacg
    12481 tggcagtcga gatagatatc aatatcggtt tgttgatcga gatcctgagc aatgtatggc
    12541 tgccagtgtg cgggatagac caggtcgata ataccgagtg aaatattgtt gatctgttcc
    12601 tgatcgtttt cgccaaacaa tccccaacga cggggtgtac cgcgcagaca atgaatttcc
    12661 cgcagggagg attttgccgg gggcgcgaat agcccccaca gacctgcgcc cagcagcagt
    12721 aatgcgccgc ctgccagcca cggaacaaat acatccgggg taatcaggca gaaaaagaac
    12781 atcaggaagg aggcgacgat cagcaacgct tcacgcagcc cgcgcggacg actcagcgca
    12841 tattcttcat gcgtttcttt gcgaatattg agtagctcga tttgctcact ctcttccccg
    12901 cgaatcgacg cctgcgtgga cggaacaggt tgcaaggcat agccgcgcgt ttcctgcatg
    12961 tactcctgta gcgtatgacc gttgagtgaa ataaccaacg gcagcgaatc ggtatgaatc
    13021 agttcaacgg tattctcatc gttgatgtac tgttcccaga aggggggcag gtggacttct
    13081 accgaatcga ggtagtaacg ccatttattc ggatcgtcgg tagagatgcc gtaacgtgtg
    13141 atagcgtgtg tcagcatcat gacgttgttg ctttcggcat tcagcgccag agagatcggt
    13201 gccgcgctag ctcccgttgg gccaggcacc tgtaatacct gcgtcaggct ctcaagataa
    13261 ttttcaacgg cgctacgttc ttcaggtgtg agtttacgcg tttgcgcatc cgcgaaggcg
    13321 ttggtccagg gcagctgact ccgtctggat cgcactttta tcagccatcc cgcaagtagt
    13381 gagcaggcca gcaaagcagc taaaaaaatc acaatggtgc tcatgctttc cccatcttac
    13441 ttagtctgtc aggcgtggtc agtcgcacca gacacaatac atgattctgc ggttggctat
    13501 cggcaagcgt ggagtttaac ttgagcatca ttaagcacat cacagtaacc gctgatcata
    13561 gcaaaccctc tgaaggcgga atatcagcaa attcctagag ttatttcaac ctattgactt
    13621 ttaatttgat ttgaggacga ttatttatta agttacataa atgtaaataa catgcaattc
    13681 acattgccga aagcgtgtcc gatatcgcac aataaccgct gatttcacag catagatccc
    13741 acgatgagca aatcattaca aaaacccacc attctgaatg ttgaaactgt agcccgttcc
    13801 cgactgttta ccgtcgagag cgtggatctg gagttcagca atggcgtgcg gcgtgtttat
    13861 gaacgaatgc gtccaaccaa ccgggaagca gtgatgattg tgccgattgt ggacgatcac
    13921 ctgatcctga tccgcgaata cgcagtggga actgaatcct acgaattagg tttttcgaaa
    13981 ggattaattg atccgggtga aagcgtctac gaagccgcta accgcgagct aaaagaagag
    14041 gttggatttg gtgcgaacga tctgactttt ttgaagaagc tcagcatggc accgtcttac
    14101 ttttccagca aaatgaatat cgtggtcgcg caagatctct acccggaatc gctggaaggc
    14161 gatgagccag agccgctacc acaggtgcgc tggccgctgg cgcatatgat ggatttgctg
    14221 gaagaccctg acttcaacga agcgcgtaat gtcagcgcgc tgttcctcgt gcgcgaatgg
    14281 ttgaaagggc aggggcgagt gtaaatgcgt gaagtttgcc tgatgcacaa cgcttatcag
    14341 gcttatgggg ttcctgcaat atattgattt ttcacgattt tgttagccgg gtaacgcggt
    14401 cacccgcatc cagcataaac aaatgcactt tgtcagccaa ctgaaaaggc gccgaagcgc
    14461 ctttttaatc agaacaattc ctgtgcctca ccattatcga taatggtcgt tcccacctcg
    14521 tgcaccgcct gttgtgtcgg ctgcgtacct tcgatgaaat actcttcgcg gctgttgcca
    14581 ccattagcta actgcccggt gctgcgatcg atattcaccg tcacaatacc cggtggcggc
    14641 gtcagcggct gctccggcac accttcaaga acggctttca tataagcgtc ccatgcaggc
    14701 tgggcactct tggctccgcc ttcgtaacct gagatctgat ctttaatcgc tccggaagcc
    14761 gttgtatgac cgagattacg acggtgatca tcaaagccaa tccagaccga ggtcacaacg
    14821 cccggaccgt aacccgagaa ccacgcatct ttcgaactgt tagtggtacc ggttttcccg
    14881 ccgatatcgc gacgctgcaa atcacgacct gcacgccagc cagtaccctg ccagcctggc
    14941 tcaccaaaga tattggtgtt caaagcactc ttaatcagga atgccagcgg agtgttgatg
    15001 acgtgcggtg cgtactcctg cgcgccagtc ttcgccacta acgcctgatt tgcctgctcc
    15061 agctgcggca ttggtacaga aacattctgc tgctcgcggg agatagcgac atcttcaaca
    15121 tcgttatttt ccagcacgtt cgatttctgc gtatcaccgt aaatcaccgg aatatcgcat
    15181 tccgggcagg ctactttcgg tttcgcttcg aaaatcacgc cgccctgatc gttttcaatt
    15241 ttgctgataa accacgggtc caccaggaag ccgccgttcg ccatgaccgc gtagccgcgc
    15301 gccacctgca ttggggtgaa ggacgctgaa cccagcgcca gcgattcggt gtggacaatg
    15361 ttttgcgccg ggaagccgaa gcgttgcaga tattctgcag cgtagtcgac gcccatagcc
    15421 cgcattgcac gtaccatcac cacgtttttc gactgaccca gcccctgacg taagcgaatt
    15481 ggaccagcat attgtggcgg cgagtttttc ggctgccagt cagaaccggc acctgcgtcc
    15541 cagcgagaaa ttggcacatc gttcaacata cttgccagcg tcagaccttt atccatcgcc
    15601 gcggtgtaga ggaacggttt gatgttggaa cccacctgac gcagtgcctg ggtggcgcgg
    15661 ttaaacttgc tctgattgaa atcaaagcca ccgaccagcg ccataacggc accgttttgc
    15721 ggattgatcg acaccagcgc cgagttcact tccggcactt gtgccagcca ccatgcatcg
    15781 ccaacctgac gaacccagat ttgctgaccc gtttgcagaa catcggtcac tttacgcggc
    15841 gtcggtccct gctgagtatc cgaacggtaa ggacgcgccc agcgaacgcc ttccatactc
    15901 aatgcgacgg tcgacccgtc cgccagcatc gccgtcgctc cctgaggatt ggcgctggtg
    15961 actgcggcag gcagcagcgg accataggtc ggcagcgcct tcagcgtatc ggtaatcttg
    16021 ttgttatccc acgccgactc gcccactttc cacagcacat ttgccgggcc gcgatagccg
    16081 tggcgcatgt cgtagtccag cacgttatta cgtaccgcct gctgtgcggc ctgctgcact
    16141 ttgcgggtga tggtggtgta aatgcgataa ccgtcttcat aggcactttc gccataacgg
    16201 ttatacatct cctggcgcac catttcgctc aggtacggcg cagagaaagc aatctccggc
    16261 gcgtgatagt tagcgttaat cgcctcagtg cgtgtctgat cgaactgttg ttgggtgata
    16321 tacccttcat ccagcatccg cgacagtacg acgttacgcc gcgcgacggc acgatccatt
    16381 gagtagagcg gattaaaggt ggaaggcgct ttcggcagcc cggctatcac agccatttcg
    16441 ttcagcgtca gttggtcgac cgttttaccg aaatagactt gtgccgcagc accgacacca
    16501 taggcgcggt aaccgaggta aatcttattc agataaagct cgaggatctc gtctttcgtc
    16561 agcaactgtt caatgcgaat cgcgaggaag acttccttaa tcttacgcat cagcgtgcgt
    16621 tctggactga ggaagaagtt tctcgccagc tgctgggtaa tggtactcgc cccttgtgac
    16681 gcgtggccgg agaacagcgc cacacttgcc gcacggaaga tccccaccgg gtcaacgccg
    16741 tgatgctcgt agaagcggct gtcttccgtc gcgataaagg ctttcaccat ctccggcggg
    16801 atttgatcca acgtaaccgg aatacgacgt ttctcaccgt attgagcaat cagctcgcca
    16861 tcggcgctgt aaatctgcat cggaatttgc aggcgaacat cttttaatgt cgccacatcc
    16921 ggcagttgtg gctcgatgta gcggtatagg ccataaatcg agcctgctcc cagcagaatg
    16981 caacagactg caaggatcaa aaaatacttt acgaacttca ctggaaattt cccatttagt
    17041 ttcatttggg cagtttataa acaaacgcgc ggtagtataa aggcaagcca gacgcattga
    17101 tatacccgtc agagtgacgg gtgataagga gatcatcaca atggcattta agatctggca
    17161 aattggtttg catttacaac agcaagaagc ggtagcggtt gcgatcgtgc ggggcgcaaa
    17221 agaatgcttt ttgcaacgct ggtggcggtt gccgctggag aacgacatta tcaaagatgg
    17281 gcggattgtt gatgcgcagc agctggctaa aacgttgtta ccttggagtc gcgaactgcc
    17341 gcagcgtcat cacattatgt tggcgtttcc cgccagtcgc acattacagc ggtcatttcc
    17401 gcgcccgtcg atgtcccttg gtgagcggga gcaaacggcc tggctgtcag ggacgatggc
    17461 ccgcgagctg gatatggatc cggactccct gcgcttcgat tacagcgaag actcactcag
    17521 tcccgcctat aacgtgactg ccgcgcaaag caaagagctg gcaacgctgc ttactctggc
    17581 agaaaggttg cgtgttcatg ttagtgcgat caccccggat gccagtgcat tacagcgatt
    17641 cctgcctttt ttaccttctc atcagcaatg tctggcctgg cgtgataacg aacagtggct
    17701 gtgggcgacg cgctatagct gggggcgcaa actggcggtg gggatgacta gcgcgaagga
    17761 gctggcggca gcgttattcg ttgatcccga cagcgtcgcg atatgtggcg aaggcggatt
    17821 tgacccctgg gaggccgttt ctgttcgtca gccgctgcta ccgccgcctg gtggagactt
    17881 tgccatcgcg ctggggctgg cgcttgggaa ggcgtactga tgaacccgcc aattaatttt
    17941 ttgccctggc gacagcaacg ccggaccgct tttctgcgtt tctggttgct gatgttcgtt
    18001 gcgcctctgc tgctggccgt cgggataacg ctaatactgc gtctgacagg cagcgccgaa
    18061 gcgcgcatag acgcagtttt gcttcaggcg gaacaacaac tcgcccgcag cttacagata
    18121 acgaagccac gtttgctgga gcaacagcag ttacgcaaac agcgttcgca acggcagcgc
    18181 cagcgacaat ttacccgcga ctggcaatct gcgctggaag cactggcggc gcttttaccc
    18241 gagcacgcct ggctgacaac gataagctgg cagcagggaa cgctggagat caaggggctg
    18301 acaacaagca ttaccgcgtt aaacgcacta gaaacgtcac tccgccagga tgcgtctttt
    18361 catctcaatc agcggggagc cacgcagcag gatgcgcagg gacgctggca atttgagtat
    18421 cagttaacaa ggaaggttag cgatgaacat gttctttgac tggtggttcg ccacatcacc
    18481 ccgcctccgc cagttttgct gggcattctg gttgctgatg ttagttacgc tcatttttct
    18541 gtcatcgaca caccatgaag agcgcgacgc attaattcga ttacgggcaa gtcatcacca
    18601 gcagtgggcc acactgtatc gcctggttga caccactccc ttcagcgaag aaaaaacgct
    18661 gcccttttcg ccactggatt ttcagttgcc cggcgcgcaa ctggtttcct ggcatccatc
    18721 cgcgcaggga ggtgagttgg cgttgaaaac gctgtgggaa gcagtgccgt cggcattcac
    18781 acggctggca gagcgcaacg tcagcgtgag ccgtttttcg ttaagcgtgg aaggtgatga
    18841 tcttttgttc acgctacaac tggagacgcc gcatgagggt taaacgctgg ttgttggcag
    18901 gtattgcatt gtgcctttta accggtatgc gtgacccttt taaaccgccg gaagatctat
    18961 gccggattag cgaacttagc cagtggcgct atcaggggat ggtagggcga ggcgagcgca
    19021 tcatcggtgt aataaaagac gggcaaaaga aatggcgacg ggtgcagcaa aacgatgtgc
    19081 tggaaaacgg ctggacaatt ttacagctga cgccagacgt actaacgctg ggtaccggga
    19141 caaactgcga accgccacaa tggttgtggc aacggcaagg agatacaaat gaagcaatgg
    19201 atagccgcac tactgttgat gctgataccc ggcgtacagg cggcaaagcc gcaaaaagtg
    19261 acgctgatgg tggatgacgt tccggtagct caggtgttgc aggcgctggc tgaacaggag
    19321 aagttgaacc tggtcgtgtc gccagacgtc agcggtacgg tgtcgttaca tctaacagat
    19381 gttccctgga agcaggcact acaaactgta gtgaaaagcg ccggactgat aacgcggcag
    19441 gaaggcaaca ttctctcagt gcattccatt gcctggcaga ataacaatat cgcccgccag
    19501 gaggcggagc aggcgcgggc gcaggcaaat ctgccgctgg aaaatcgcag tataaccctg
    19561 caatacgccg acgcgggaga actggcgaaa gcgggggaga agctactgag tgccaaaggg
    19621 agtatgaccg tcgataaacg caccaatcgc cttttgctac gagataacaa aacggcgtta
    19681 agcgcgcttg aacagtgggt agcgcaaatg gatctgccgg tcgggcaggt tgagctgtcg
    19741 gcgcatattg tcaccattaa tgaaaaaagt ttgcgtgagt taggcgtgaa atggacgctg
    19801 gccgatgcgc aacacgctgg tggcgttggg caagtcacca cgcttggtag cgacctctcc
    19861 gtagcgacgg cgacaacgca tgtcggtttt aacattgggc gcatcaacgg acgcttgctg
    19921 gatcttgagc tttccgcgct cgaacaaaaa cagcagctgg atattatcgc cagtccgcgt
    19981 ctgctggcct cacatcttca gcctgccagc attaaacagg ggagcgaaat tccatatcag
    20041 gtttccagcg gggaaagtgg cgcgacgtcg gtggaattta aagaggccgt cctggggata
    20101 gaggtcacgc ccacggtgtt acaaaaaggt cgcatccggc tgaaattaca catcagccag
    20161 aacgttccgg ggcaggtgct acagcaggcc gatggcgaag tgctggcgat tgataagcag
    20221 gagatcgaaa cgcaggtcga ggtcaaaagc ggagaaacgt tggcgctggg cggcattttt
    20281 acccgtaaaa ataaatcggg tcaggatagc gtaccgttgc ttggcgacat tccctggttc
    20341 gggcaattat ttcgtcatga cggaaaagaa tatgaacgac gcgagttagt ggtgtttatc
    20401 acgccacgac tggtttccag tgagtaaaca gccgtaaaag cggtaatgtt tttatgctga
    20461 acgtgtttca tctatttgac gcgcgcaggt atttagcata caaggagtac cgatttgaga
    20521 gttggcgctc ttcgctgcct gcgttccatg atgatgattt atcattcagg cggcattttg
    20581 ctgtcttttt tacgctaatc ttacccggtg atttatcgcc agagcggtgg tagcaaggca
    20641 gcgcgcttgc agcgaccaga tatgcagagg gatgggtgat ttattcagtt gccaaacccg
    20701 ctggagtatt gagataattt tcagtctgac tctcgcaata tcttatgagg tttcagttca
    20761 tgtcctgcgg cgctctctga gcgaagcggg tttatcatta acgaatagtc ttagtagtac
    20821 cgaaaaaatg gcagagaaac gcaatatctt tctggttggg cctatgggtg ccggaaaaag
    20881 cactattggg cgccagttag ctcaacaact caatatggaa ttttacgatt ccgatcaaga
    20941 gattgagaaa cgaaccggag ctgatgtggg ctgggttttc gatttagaag gcgaagaagg
    21001 cttccgcgat cgcgaagaaa aggtcatcaa tgagttgacc gagaaacagg gtattgtgct
    21061 ggctactggc ggcggctctg tgaaatcccg tgaaacgcgt aaccgtcttt ccgctcgtgg
    21121 cgttgtcgtt tatcttgaaa cgaccatcga aaagcaactt gcacgcacgc agcgtgataa
    21181 aaaacgcccg ttgctgcacg ttgaaacacc gccgcgtgaa gttctggaag cgttggccaa
    21241 tgaacgcaat ccgctgtatg aagagattgc cgacgtgacc attcgtactg atgatcaaag
    21301 cgctaaagtg gttgcaaacc agattattca catgctggaa agcaactaat tctggcttta
    21361 tatacactcg tctgcgggta cagtaattaa ggtggatgtc gcgttatgga gaggattgtc
    21421 gttactctcg gggaacgtag ttacccaatt accatcgcat ctggtttgtt taatgaacca
    21481 gcttcattct taccgctgaa atcgggcgag caggtcatgt tggtcaccaa cgaaaccctg
    21541 gctcctctgt atctcgataa ggtccgcggc gtacttgaac aggcgggtgt taacgtcgat
    21601 agcgttatcc tccctgacgg cgagcagtat aaaagcctgg ctgtactcga taccgtcttt
    21661 acggcgttgt tacaaaagcc gcatggtcgc gatactacgc tggtggcgct tggcggcggc
    21721 gtagtgggcg atctgaccgg cttcgcggcg gcgagttatc agcgcggtgt tcgtttcatt
    21781 caagtcccga cgacgttact gtcgcaggtc gattcctccg ttggcggcaa aactgcggtc
    21841 aaccatcccc tcggtaaaaa catgattggc gcgttctacc agcctgcttc agtggtggtg
    21901 gatctcgact gtctgaaaac gcttcccccg cgtgagttag cgtcggggct ggcagaagtc
    21961 atcaaatacg gcattattct tgacggtgcg ttttttaact ggctggaaga gaatctggat
    22021 gcgttgttgc gtctggacgg tccggcaatg gcgtactgta ttcgccgttg ttgtgaactg
    22081 aaggcagaag ttgtcgccgc cgacgagcgc gaaaccgggt tacgtgcttt actgaatctg
    22141 ggacacacct ttggtcatgc cattgaagct gaaatggggt atggcaattg gttacatggt
    22201 gaagcggtcg ctgcgggtat ggtgatggcg gcgcggacgt cggaacgtct cgggcagttt
    22261 agttctgccg aaacgcagcg tattataacc ctgctcaagc gggctgggtt accggtcaat
    22321 gggccgcgcg aaatgtccgc gcaggcgtat ttaccgcata tgctgcgtga caagaaagtc
    22381 cttgcgggag agatacgctt aattcttccg ttggcaattg gtaagagtga agttcgcagc
    22441 ggcgtttcgc acgagcttgt tcttaacgcc attgccgatt gtcaatcagc gtaacaacaa
    22501 gaaaggtcag gccgcttatc aagcggtcta ttagcttcag gttaattgca acgtggtaag
    22561 cattaacctt ttagtggggt gttaaatgga tgaattcaaa ccagaagacg agctgaaacc
    22621 cgatcccagc gatcgtcgta ctggtcgttc tcgtcaatct tctgaacgtt ctgagcgtac
    22681 tgaacgtggc gaaccgcaga tcaattttga tgatattgaa cttgatgaca ctgacgatcg
    22741 ccgtccgact cgtgcgcaaa aagagcgcaa tgaggaaccg gaaatcgaag aagaaattga
    22801 cgaatccgaa gatgaaaccg tggatgaaga gcgcgtagag cgtcgtccgc gtaagcgcaa
    22861 aaaagcagcc agtaaacccg cttctcgtca gtatatgatg atgggcgtcg gcattctggt
    22921 tctactgctg ttgatcatcg gtatcggttc tgcgctaaaa gccccctcga cctcttccag
    22981 cgatcaaacc gcgtctggcg ataagagtat tgatcttgca ggcaatgcga ccgatcaggc
    23041 gaatggggtg cagccagcgc cgggaaccac gtctgcggaa aatactcagc aggatgtttc
    23101 tctgccaccg atctcttcta cgccgactca agggcaaacc ccggcggcaa cggatggtca
    23161 acaacgtgtt gaagtgcagg gtgacctgaa caatgcgctg actcagccac aaaatcagca
    23221 acagttgaac aatgtggcgg tcaattccac attgccgacc gaacccgcga ctgtcgcgcc
    23281 tgttcgcaat ggcaatgcat cgcgtgacac ggcgaaaacg caaaccgctg aacgtccgtc
    23341 cactacgcgc ccagctcgtc agcaggcggt gattgaaccg aaaaaaccgc aagcaaccgt
    23401 gaaaacggag ccgaagccgg tagcacagac gccgaagcgt actgaaccag ctgcccctgt
    23461 ggcgagcacg aaggcaccgg ctgcgacttc tacgccagca ccaaaagaga cggcgactac
    23521 ggctccagta cagacggcat ccccggcgca aaccacggca acaccagccg ctggagggaa
    23581 gaccgcaggt aatgttggtt cgttgaaatc ggcaccgtcc agccattaca ctctgcagct
    23641 gagcagttcc tctaactacg acaacctgaa cggttgggcg aagaaagaga atctgaaaaa
    23701 ctacgttgtc tatgaaacga cgcgtaatgg tcagccgtgg tatgtcctgg tttctggcgt
    23761 gtacgcttcg aaagaagagg cgaaaaaagc ggtatctaca ttgccagcag atgttcaggc
    23821 caaaaacccg tgggcgaaac cgctgcgtca ggtacaggcc gatctgaagt aatcaaggtt
    23881 atctcccgca atggtttatc gttgcgggag ctgcctgaag cgctggatgc tgtcggagct
    23941 ttctccacag ccggagaagg tgtaattagt tagtcagcat gaagaaaaat cgcgcttttt
    24001 tgaagtgggc agggggcaag tatcccctgc ttgatgatat taaacggcat ttgcccaagg
    24061 gcgaatgtct ggttgagcct tttgtaggtg ccgggtcggt gtttctcaac accgactttt
    24121 ctcgttatat ccttgccgat atcaatagcg acctgatcag tctctataac attgtgaaga
    24181 tgcgtactga tgagtacgta caggccgcac gcgagctgtt tgttcccgaa acaaattgcg
    24241 ccgaggttta ctatcagttc cgcgaagagt tcaacaaaag ccaggatccg ttccgtcggg
    24301 cggtactgtt tttatatttg aaccgctacg gttacaacgg cctgtgtcgt tacaatctgc
    24361 gcggtgagtt taacgtgccg tttggccgct acaaaaaacc ctatttcccg gaagcagagt
    24421 tgtatcactt cgctgaaaaa gcgcagaatg cctttttcta ttgtgagtct tacgccgata
    24481 gcatggcgcg cgcagatgat gcatccgtcg tctattgcga tccgccttat gcaccgctgt
    24541 ctgcgaccgc caactttacg gcgtatcaca caaacagttt tacgcttgaa caacaagcgc
    24601 atctggcgga gatcgccgaa ggtctggttg agcgccatat tccagtgctg atctccaatc
    24661 acgatacgat gttaacgcgt gagtggtatc agcgcgcaaa attgcatgtc gtcaaagttc
    24721 gacgcagtat aagcagcaac ggcggcacac gtaaaaaggt ggacgaactg ctggctttgt
    24781 acaaaccagg agtcgtttca cccgcgaaaa aataattctc aaggagaagc ggatgaaaca
    24841 gtatttgatt gccccctcaa ttctgtcggc tgattttgcc cgcctgggtg aagataccgc
    24901 aaaagccctg gcagctggcg ctgatgtcgt gcattttgac gtcatggata accactatgt
    24961 tcccaatctg acgattgggc caatggtgct gaaatccttg cgtaactatg gcattaccgc
    25021 ccctatcgat gtacatctga tggtgaaacc cgtcgatcgc attgtgcctg atttcgctgc
    25081 cgctggtgcc agcatcatta cctttcatcc agaagcctcc gagcatgttg accgcacgct
    25141 gcaactgatt aaagaaaatg gctgtaaagc gggtctggta tttaacccgg cgacacctct
    25201 gagctatctg gattacgtga tggataagct ggatgtgatc ctgctgatgt ctgttaaccc
    25261 aggttttggc ggtcagtcgt tcattccaca aacactggat aaactgcgcg aagtacgtcg
    25321 tcgtatcgac gagtctggtt ttgacattcg actggaagtg gacggtggcg tgaaggtgaa
    25381 caacattggc gaaatcgctg cggcgggcgc ggatatgttc gtcgccggtt cggcaatctt
    25441 cgaccagcca gactacaaaa aagtcattga tgaaatgcgc agtgaactgg caaaggtaag
    25501 tcatgaataa gtttgaagat attcgcggcg tcgcttttga tctcgacggt acgctggttg
    25561 acagtgctcc cggtcttgct gctgcggtag atatggcgct gtatgcgctg gagttacccg
    25621 tcgcaggtga agaacgcgtt attacctgga ttggtaacgg cgcagatgtt ctgatggagc
    25681 gtgcattggc ctgggcgcgt caggaacgtg cgactctgcg taaaacaatg ggtaaaccgc
    25741 ccgttgatga cgacattccg gcagaagaac aggtacgtat tctgcgtaaa ctgttcgatc
    25801 gctactatag cgaggttgcc gaagagggga cgtttttgtt cccgcacgtt gccgatacgt
    25861 tgggcgcgtt gcaggctaaa ggcctgccgc taggcctggt caccaacaaa ccgacgccgt
    25921 tcgtcgcgcc actgctcgaa gccttagata ttgccaaata ctttagcgtg gttatcggcg
    25981 gcgatgatgt gcaaaacaaa aaaccgcatc cggacccgct gttactggtg gctgagcgga
    26041 tgggaattgc cccacaacag atgctgtttg tcggcgactc acgcaatgat attcaggcgg
    26101 caaaagcggc aggttgccca tcagttggct taacctacgg atataactac ggcgaggcta
    26161 tcgatctcag ccagcctgat gtaatttatc agtctataaa tgaccttctg cccgcattag
    26221 ggcttccgca tagcgaaaat caggaatcga aaaatgacta agcccatcgt ttttagtggc
    26281 gcacagccct caggtgaatt gaccattggt aactacatgg gtgcgctgcg tcagtgggta
    26341 aacatgcagg atgactacca ttgcatttac tgtatcgttg accaacacgc gatcaccgtg
    26401 cgccaggatg cacagaagct gcgtaaagcg acgctggata cgctggcctt gtatctggct
    26461 tgtggtatcg atcctgagaa aagcaccatt tttgttcagt cccacgtacc agaacatgcg
    26521 cagttaggct gggcactgaa ctgctatacc tacttcggcg aactgagccg catgacccag
    26581 tttaaagata aatctgcgcg ttatgccgag aacatcaacg ctggtctgtt tgactatccg
    26641 gtgctgatgg ctgcggacat cctgctgtat caaactaatc tggtaccggt gggtgaagac
    26701 cagaaacagc acctggaact gagtcgcgat atcgcccagc gtttcaacgc gctgtatggc
    26761 gagatcttta aggtgccgga gccgtttatt ccgaaatctg gcgcgcgcgt aatgtcgctg
    26821 ctggagccga ccaagaagat gtccaagtct gacgataacc gcaataacgt tatcggcctg
    26881 ctggaagatc cgaaatcggt agtgaagaaa atcaaacgcg cggtcactga ctccgacgag
    26941 ccgccggtag ttcgctacga tgtgcagaac aaagcgggcg tttccaacct gctggatatc
    27001 ctttctgcgg taacgggcca gagcatccca gaactggaaa aacagttcga aggcaagatg
    27061 tatggccatc tgaaaggtga agtggctgat gccgtttccg gtatgctgac tgaattgcag
    27121 gaacgctatc accgtttccg caacgatgaa gccttcctgc aacaggtgat gaaagacggc
    27181 gcagaaaaag ccagtgtaca cgcttcccgt acgctgaaag cggtgtacga agcgattggt
    27241 tttgtggcga agccgtaata tcaaaacgct cggacggtcc cctcgcccct ttggggagag
    27301 ggttagggtg aggggagcag gcaagcgcca gtgggaatta ccctttgctc gcattgtcga
    27361 ataaatcact tcgtcatcac tctgaaaccg gggaaacccg gtttttttat cctcatttgc
    27421 aataatccga aaaaatgtga agcgcctcgc cgtttccaca tctcacagtt gccacttatc
    27481 ttcattttaa tgaagatata aacattcatt ttattgaaaa tatattatgc gtcgaacgtt
    27541 tatcaaaaaa gaaggcgtcg tcatcacgac gctggcccgt tatttgttgg gtgaaaaatg
    27601 cggtaatcga ctgaaaacca tagatgagct ggcaactgaa tgccgttcat cggttggcct
    27661 gacgcaggcc gcgttgaaaa cgttggaatc aagtggagcg atacggattg aacgccgtgg
    27721 gcgcaatggc agttatctgg tcgaaatgga taacaaagcg ctgctgagtc atgtggatat
    27781 caataacgtt gtgtgtgcca tgcctttgcc ctatacccgc ttgtacgaag gtttagcaag
    27841 cgggctgaaa gcccagtttg atggcattcc tttttattat gcgcatatgc gtggcgcgga
    27901 tattcgtgtg gagtgtttgc ttaacggtgt ctatgacatg gcagtagttt cccgcctggc
    27961 ggcggaaagt tatctcagcc aaaacaattt gtgcatcgcc ctcgagcttg gcccgcatac
    28021 gtatgttggt gaacatcaac tgatatgccg caaaggtgag tctggaaatg ttaagcgagt
    28081 aggtctcgat agtcgttcgg cggatcagaa aatcatgacc gatgtctttt ttggcgatag
    28141 tgatgtggaa cgtgtcgatc tctcttatca cgagagttta caacgcattg ttaaaggcga
    28201 tgtcgatgcg gttatctgga acgtggtggc ggaaaacgaa ctgaccatgc tgggattaga
    28261 ggcgacgccg ctcacagacg atccgcgatt tttacaggcc accgaagccg tggtcctgac
    28321 gcgagtcgat gattacccaa tgcaacaact gctgcgtgcc gttgtagata aacacgccct
    28381 gctcgcccat caacaacggg tagtgagtgg ggaacaggaa ccgagttatt aaacgaaagg
    28441 catctgatat ggaaaccaga ctcaacctgc tttgcgaggc aggcgttatt gataaggaca
    28501 tctgcaaagg catgatgcag gtcgtcaacg tactggaaac agagtgccat ctgccggtgc
    28561 gcagtgagca aggaacgatg gcgatgacac atatggcgag tgcactgatg cgcagtcgcc
    28621 gtggtgaaga aatagagccg ctggataacg aattgctggc agagctggcg caatccagcc
    28681 actggcaagc cgttgtgcaa ttgcatcagg tgttgttgaa ggaattcgca ctggaagtta
    28741 atccgtgtga agaaggctat ttgctggcca acctttatgg attatggatg gctgctaacg
    28801 aagaggtttg aagctcccga caaatccacg tcgcacaacc taaatattca aaaaaatgaa
    28861 tatttaattc aaggaacaga aacgaggcaa caagatgttt gtagaagcat tgaaacgcca
    28921 gaacccggcg ctgatttccg ccgcactaag tttgtggcag cagggcaaga tcgccccgga
    28981 cagctgggtg atcgacgtgg atcaggtact ggaaaacggt aagcgactga ttgagacggc
    29041 gcggctttac ggcattgaac tgtatctgat gaccaagcag tttggccgta acccatggct
    29101 ggcggaaaaa ttgctggcat taggttacag cggcattgtg gcggtggatt acaaagaggc
    29161 gcgagtcatg cgccgcgctg gtttgtcggt ggcgcatcag gggcatctgg tacaaatccc
    29221 ttgtcatcag gttgctgacg ccgttgaaca gggcaccgac gtcatcaccg tgtttactct
    29281 cgacaaagcg cgggaagttt ctgcggcagc ggtgaaggcc ggacgtgtgc agtttgtgct
    29341 gctcaaagtt tatagcgacg atgattttct ttatccgggg caggagagcg gttttgtaca
    29401 gcattcgctg cacgaggtgg ttgctgaaat taagaaactg ccgggtctgc atttagccgg
    29461 acttacccat ttcccttgcc tgctttggga tgaggctgcc ggaaaagttt tgccgacacc
    29521 gaatcttcac acgctgatac aggcacggga tcaactggcg aaatctggta ttgcacttga
    29581 gcaactgaac gcgccttcag cgaccagctg cacttcgctg ccattactgg cgcaatacgg
    29641 tgtgactcat gccgaacccg gtcatgcgct gacgggcact attccggcaa accagcaggg
    29701 cgatcagtct gaacgtatcg cgatgctctg gttgagtgaa atctcccatc atttccgtgg
    29761 cgacagctac tgctacggcg gcggttacta ccgtcgtggt catgcgcaac atgcgttggt
    29821 gtttacgcca gaaaatcaaa agattactga aaccaatctc aaaactgtgg atgacagcag
    29881 tatcgactac accctgtcgc tggcaggcga gtttccggta agcagtgcag tggtgctctg
    29941 tttccgcacg cagatttttg tcacccgtag cgatgtggtg ctggtgtccg gtattcaccg
    30001 tggcgaaccg aaaatcgttg gtcgttatga cagtcttgga aactctctgg gggcgtaatg
    30061 gcgcgatttg tggtgttagt gattgatagc tttggcgtag gggcaatgaa agatgtcacg
    30121 ctggtgcgtc cgcaagatgc gggagcgaat acatgtggtc acatcctgag ccagttgccg
    30181 catttgcagc taccaacgct ggagaagctg gggctaatca acgcattggg ttatgcgcca
    30241 ggcgatatgc agccgtcaga ttccgcaacc tggggcgtgg cagagctgca acatgaaggt
    30301 ggcgatacct ttatggggca tcaggaaatt ttaggcacgc gcccgttacc gccgctgcgg
    30361 atgccttttt gcgatgtgat tgaccgtgtt gagcaggcat tagtatccgc cggttggcag
    30421 gtggagcgcc gtggcgatga actgcaattt ctgtgggtca atcaggcggt tgcgattggc
    30481 gataatctcg aggcggattt aggccaagtc tataacatta ccgccaatct ctctgtgatc
    30541 tcttttgacg acgcaatcaa aattggtcgt atcgtgcgtg agcaggtaca ggtcggtcgg
    30601 gtcattacat ttggtggcct gttaaccgac agtcaacgca ttctcgatgc cgcagaaagc
    30661 aaagaagggc gctttattgg tatcaatgcg ccgcgttctg gcgcttatga caacggcttc
    30721 caggtcgtgc atatgggcta cggtgtcgat aaaaaagtgc aggtgccgca aaaactgtat
    30781 gaggcgggcg tgccaaccgt gctggtgggt aaggtggcag atatcgtcag caatccttat
    30841 ggcgtgagct ggcaaaacct ggtggatagc cagcggatta tggatatcac cctcgacgaa
    30901 tttaacaccc atccgacggc gtttatttgc accaatatcc aggaaaccga cctcgctggt
    30961 catgcagaag acgtcgcacg ttatgccgaa cgtttgcagg tcgttgaccg taaccttgcc
    31021 cggcttgttg atgcgatgca gccagatgat tgcctggtcg tgatggcgga tcacggcaac
    31081 gatccgacca ttggtcacag ccaccatacc cgcgaagtgg tgccgctgct ggtttatcag
    31141 caaggcctgg ctgctacgca gctcggtgtg cgcaccacgc tttctgatgt gggagctacc
    31201 gtgtgtgaat ttttccgtgc gccaccgcca caaaatggtc gctcttttct ttcctccctc
    31261 cggtttgcag gagacaccct atgagttttg atccgacggg ttacaccctg gcccatgagc
    31321 atctgcatat tgatctctcc ggctttaaaa acaacgtgga ctgccgcctt gatcagtatg
    31381 cgttcatttg ccaggagatg aacgacctga tgacccgggg tgtacgtaat gtgattgaga
    31441 tgaccaaccg ttacatgggg cgcaatgcgc aatttatgct cggtgtaatg cgcgaaaccg
    31501 ggatcaacgt ggtggcctgt actggttatt accaggatgc gtttttcccg gaacatgtgg
    31561 tgacccgcag cgtgcaggaa ctggcgcagg agatggtcga tgaaattgaa cagggtatcg
    31621 atggcacgga gctgaaagcc gggctcatcg cggagatcgg ctccagcgaa ggaaagatta
    31681 cgccgctgga agagaaggta tttattgccg ctgcgctggc gcataaccag accggacgcc
    31741 cgatctccac gcatacgtcg ttcagcacca tggggctgga acaactggct ctgttgcaag
    31801 cccacggagt tgatctttcg cgcgtcaccg ttggtcactg cgatctgaaa gacaacctcg
    31861 acaacatttt gaagatgatc gatctcggcg cgtacgtgca gttcgacacc attggtaaga
    31921 acagttacta cctggacgaa aagcgcattg cgatgctcca cgcgctacgt gaccgtgggt
    31981 tgctgaaccg cgtcatgctg tcgatggata ttacgcgccg ctcccattta aaagccaatg
    32041 gtggttatgg ctatgactat ttattgacca cctttattcc gcaattgcgc cagtcaggat
    32101 tcagtcaggc cgatgtggat gtgatgttac gtgaaactcc ctctcaattt ttccaataag
    32161 gacagactca tgaaaaagat tggcgttgca ggcttacagc gtgagcagat taaaaaaacc
    32221 attgaagcga cggctcctgg cagttttgaa gttttcattc acaacgacat ggaagcggca
    32281 atgaaagtga aatccgggca actggattac tacatcggtg cgtgtaatac cggtgcgggc
    32341 gcggcattgt cgattgccat cgcggtgatt ggctataaca aaagttgcac cattgccaaa
    32401 ccaggcatta aagcgaaaga cgagcatatc gccaaaatga tcgctgaagg aaaagtggcg
    32461 tttggccttt ccgttgagca cgtcgaacac gcgattccga tgctgattaa ccatctgaaa
    32521 taaaaggcac gactatggat ctgtatattc agattatcgt ggtggcgtgc ctgacgggta
    32581 tgacatcgtt tctggcgcat cgctcggcgg ctgtttttca tgacggcatc cgcccgatcc
    32641 tgccgcaact gattgaaggc tatatgaacc gtcgcgaggc ggggagtatc gcttttggtc
    32701 tgagcattgg ttttgtggcc tcggtgggga tctcttttac cctgaaaacc gggctgctca
    32761 acgcatggtt actctttctt cctaccgata tcctcggcgt actggcgata aacagcctga
    32821 tggcgtttgg tcttggcgct atctggggcg tgttgatcct tacttgcctg ttgccagtaa
    32881 accagctgct gaccgcgctg ccggtggatg tattaggtag cctcggggaa ttaagctcgc
    32941 cggtagtttc tgcttttgca ctgttcccgc tggtggcgat tttctaccag tttggctgga
    33001 agcaaagtct ggtcgccgcc gtggttgttc tgatgacccg tgtggtagtc gtgcgctatt
    33061 tcccacatct taaccctgaa tccatcgaaa tctttattgg catggtgatg ctgctgggga
    33121 tcgcgataac tcacgacctg cgtcatcgtg atgaaaatga tattgatgcc agcgggcttt
    33181 cggtgtttga agaacgcacg tcgcggatta tcaaaaactt accctatatc gccatcgtgg
    33241 gagcattgat tgccgccgtt gccagcatga agattttcgc cggcagtgaa gtgtcgatct
    33301 tcactctgga gaaagcgtac tccgcaggcg taacgccgga acaatcgcaa acgctgatca
    33361 accaggcggc actggcagaa tttatgcgcg ggctgggttt tgtaccgttg attgccacca
    33421 ccgccttagc aacgggtgtg tatgcagttg cgggctttac ctttgtttat gcggtgggct
    33481 atctctcgcc gaatccgatg gttgcagcgg tattaggcgc agtggttatt tcggcggaag
    33541 tcttgctgct tcgttctatc ggcaaatggc tgggacgcta cccgtcggtg cgtaatgcgt
    33601 cggataacat ccgtaacgcc atgaatatgc tgatggaagt ggcgctgctg gtcggttcga
    33661 ttttcgcagc aattaagatg gcgggttata ccggattctc tatcgcggtt gccatttact
    33721 tcctcaacga atccctgggc cgtccggtac agaaaatggc ggcaccggtc gtggcagtaa
    33781 tgatcaccgg tattctgctg aatgttcttt actggcttgg cctgttcgtt ccggcttaag
    33841 gaaggcacct atgaagacgt ttcctctgca aagcctgacg cttattgagg cgcagcaaaa
    33901 gcagtttgcg ctggtggaca cgatttgccg tcactttccc ggcgcggagt ttctaaccag
    33961 cggtgatttg ggcttaacgc cggggctgaa tcaaccgcgt attacccaac gggtggagca
    34021 ggtgctggct gatgcatttc acgcacaggc tgcggcgctg gtgcagggcg cggggactgg
    34081 cgcgattcgc gccgggctgg cagctttgct caaaccgggg cagcgtcttc tggtgcatga
    34141 cgcgcctgtt tacccgacga cacgggttat tattgagcag atggggctga cgcttattac
    34201 tgttgatttc aatgacctgt cggcactgaa gcaggtcgtc gacgagcaac aaccggatgc
    34261 ggcgctggtg cagcatacgc gccagcagcc gcaggacagc tacgtgctgg cagatgtgct
    34321 ggcaacgttg cgcgcggcag gtgttccagc gttaaccgat gacaactatg cggtgatgaa
    34381 ggtggcgcga atcggctgtg aatgcggcgc gaatgtctcg acattttcct gcttcaagct
    34441 atttgggcca gagggtgttg gtgcagtggt cggcgatgct gatgttatca accgtattcg
    34501 cgccacgctt tactccggcg gtagccagat ccagggcgca caggcgctgg aagtattgcg
    34561 ttgtctggtg tttgcgccag tgatgcacgc ggtgcaggca ggggtatctg aacggttgct
    34621 ggctttgctt aacggtggtg aggtgccgga agtgaaaagc gcggtgattg ctaatgcgca
    34681 gtcgaaggtg ttgattgtcg agtttcatca gccgattgcc gccagagtgc tggaagaggc
    34741 gcaaaagcgc ggtgccttgc cttacccggt gggtgcagag tcgaaatatg aaatcccgcc
    34801 gctcttttat cgcctttccg gaacgtttcg ccaggcgaat ccacaatcag aacattgtgc
    34861 gattcgcatt aacccgaatc gcagcggtga agagacggtg ctgcggattt tgcgtgagag
    34921 tattgccagt atttaatgct ggatagcacc gtcaagctaa attccgtact gaacggtccc
    34981 ctcgcccctt tggggagagg gttagggtga ggggaacgta cgggatgtaa aattcaacgc
    35041 gccagcatcg ccggatgcga tgctggcgca tcttatccgg cctacggtta atgtctttta
    35101 ttatcaatag ttaaggtgat gcgattggcg cggcagaaga gttcggagac gtgcaccggt
    35161 ttgtcattct gatcaaaggc gattttgctg atgcgaaaca acggttcgcc cagttcgcat
    35221 ttcagccatt ttgcctgcgg gcgggtggcg gcaaagatat cgatggtctt tttgtcgctg
    35281 accactcgcg tgtcaaaacg ctcctgaaat aactgatagg tggagcttcc ttcgacgtaa
    35341 atctcatcaa agtcaggata acgcgacagc gggatccatg aactatcaat aaacagcggc
    35401 tctttatcga gatacatcac ccggcagaga tggaacactt cgctgtttcc ggggatgttc
    35461 agctttttgc aaaacggcgc ggcgctgacc cgttcctgtt cgatcacttt ctctttcgtc
    35521 gccttgcctt gtgagacgcc aaaatcggta aaaccactga cagtaagcag ggcgttttca
    35581 actttctggc tttgtacaaa ggtgcctttt ccctgccagc ggatcagtac gccgtccgcc
    35641 actaagtcgc tgatggcttt gcgaatggta atgcggctga cgttatattg tgtacaaagc
    35701 tcgttttcgg tagggatctg ttgcccggcc tggtaaaccc cctgcgcgat atcatccagc
    35761 agtcgctggc ggacggtagc gtagaggagt tgatgagagt agcggtccgt agctgacata
    35821 atcgaccctg aaataaagaa tgaattactc ggattatagg gtcgttttcc ggcaagtaac
    35881 agcacgttgg cgtgaagatg ctcacgccaa cgttatacct accaggcacc gtggtactga
    35941 atggttttcg ccgcgcacgc cgttccctgc gccatcgcct gcggtaatgt catccccgca
    36001 gaccagccgc aaaggaatcc ggcaatgaac gaatctccgg cacccatggt gtcgataacc
    36061 gtcaccggtt caggagcctg acgccagaac tgcgcgccat cccaggcaat gctgccgttt
    36121 tcacccagcg tgacaatcac tgttcctgcg ccacgggcaa caatcgcttt catcttcaga
    36181 cgcagcgttt cgtcttcttg cggtgcggag gcaaaggcaa aatcgagatg cggcaccagt
    36241 gtctgccaga gcgggctgtc ccacttgtcg gagaagtcga aagcggtaag tttgcccgca
    36301 gcgtgcagct gtgggaatgc gtcttccgca tgtccccaga ttgccgcgtg cacaatgtca
    36361 tactgcgcca gccaggcgta atcctcttca ctcagggcaa agtcggccat cacgccttcg
    36421 gtgtagtcgc caaaaacgcg atcattgtcg tgcagttcca cctgagtttg tgcggtaacg
    36481 ccgtgtttcg tatggacatg gctgatatcg acgcccatgc gggcgagatc ctgcttcagc
    36541 tttgtgccgt agtcatcgtc acccacccag gtaatgcatc ccggctgtat gccgtagcga
    36601 gtgcagtaca ccgccacatt gaccgcatta ccgccagaaa acgctttatt cagttgcggg
    36661 tagatatcga cgcagttatc gccgattgtc gccagggttt tcatctctca tcctccggca
    36721 gcagcgcgcg aaagcgttcc agcgcctggc gggcatagag tctgggctcg ttcatataca
    36781 tcgtcaccag ctccaccgta cagtaaccct catagccccg ctcaataata tcgcgcatca
    36841 gttcccgcag cggcattttg ccttctccag gaatgtaatg cgtgtcgctg gccccgtcgc
    36901 tgtcgacaat atgcagatga cgtaatttat cgcccagttt gtcgaaataa ctcatcaccg
    36961 gttccgcctg gacatacggc gcgcaaatgt cgaccatgct gaacaggcgc ggcgaaggca
    37021 ccagcgccag cgcatgaagc acatcattag cattacacac gatgttcgat tcatacggcg
    37081 ttaacggttc gaggattaga tccatgccga tgttttccgc gtactcatac agttcgctta
    37141 ggttctctgc cagccgtccc cagataacat taggtggcgt gagatagccc gcgtgggccg
    37201 cggaaatcag cgtataaccc gcgttcatct cttttgccat atccatcgcc agcttgatca
    37261 tgtcgaggct ttcgcgacgc atatgttcat cgcccagcat catgttatac ggatagccat
    37321 tggtttctgg cgtatagccg ataatcggca tctgatacgt ctgcgccagc gccttgattt
    37381 gtttgatgcc gcccgctttt aagtccggcg cgaacgcgtg cgggcgaccg ccccaaattt
    37441 cgatgccgtc gtaacccagc tcgcttgcat cacgaaatgc gtgttcaata ggcagccgct
    37501 ggtggccgca ggtaaacata cctgttttca tcgggtaact ccttgcgtaa atccggcccg
    37561 ttgccgggcc gggcgaggga ttaatattcc accagaccac cgtaatagcg gcgttcatcc
    37621 gggttgtgat ctttgtaaat agacaggtag tagcagagcc actccattgg cacgaacatc
    37681 aggaacggtg ccagccacgg gtgcagccct tgcgaaattt cggcgtaatc gatgacgatc
    37741 acgttgtcag tacgctgttt aacaaagtta atggcgcgtt cggtggtgtg gcgactttca
    37801 tcattgccga gcaggaacag gaacggaacg cccggttcga caatctccag cgggccatgg
    37861 cggaactctc cgctctcaat cacgcagccg tgcgtccagg taaattccat cagcgttaca
    37921 atgccttctt tgtaacccag cggacgcagc ggacccgcag caacggtata aatcatcggc
    37981 cactggctgg ccagttcacc aagctggcgg cctttttctt cccaggtgcg taccagatga
    38041 ccgagcgcat tcggcaactg cttgagatcg tttttgatct tgccgatttc cgcgttcggc
    38101 gcgaggcggg tgatcatctc cagcaccacg ctgtagcaga gcagcaggtg aatttcccag
    38161 atacagtcgg cctgataatc aatgctaaat tccgccgccg aggtaatcgg gctgtccgcg
    38221 cgtttggtga acgccgcagt gagtgcgccg caggcccggc ccagctccag cgctttgatt
    38281 acctcttccg ttttaccgta gtcagaaacg ccaatcaccg cgcaacgatc gtcgaggcga
    38341 tacggggtgt tatcgcagaa ctcccagccg gaaatgacgt agacctgaag atcggaaaag
    38401 cgatccgcca gatgtttcgc cgcttgcgcc gcgttgagtg gcgagccgca ggcaacgaaa
    38461 taaatacgat caatgtcgcg cttcaccatc tcttccacga tggcgtgcac cagcggaacg
    38521 tcatggctga gaactttttc cacttcctga accatatttt cggtgaccag aaagtccacg
    38581 gtgcttttat caatatccaa cattttattt tctccagata acattacgac aaagcgttga
    38641 gctggcggct acgcttcgcc cagaaggcgt aagcaggcag accagtagca ataacgatga
    38701 cggcgcagat aaggccggga atcggtgccc agacaaaggt tgaggcgacc agaatgaggc
    38761 ttgacgcaat ggcgagggtg gtcatcagcc cgaaagcagg agtacgccac agcggtttgt
    38821 aatcgtcgcg tttacgacac cagatgatgg agccgaaggt gagggtattt ttgaaacaca
    38881 tcaccagggt gaaataaccc agcaggctgg tgagatcgga aacgaagatg aagaagagcc
    38941 ccagcgcccc ttgcaggatg atggagacat ccggcgtgtt gtatttcgga tgcacatggc
    39001 cgaagcattt aaagaacagg ttgtctttcg ccatcgcgta ttccagccgc ggctggtaca
    39061 tcacgcagct ggaaagcgaa ccaagaatga cgatcatcgc cgtgatggca acaaagatcc
    39121 cagcggtgct gccgagtgcg gggatccagg tcagggcgtc ggaaatcggc gtttcagaat
    39181 tggcgagttt gtcgaagggc atcaggtcgg aaatcaccag cgccagcagg gtgtagagca
    39241 ccagaaccag cagacaggaa ccaatcagcg ctcgtggcat ggtttttccg gggtttttaa
    39301 tttcgccggt catataacag atagaggcca tgccggtata cgaccaactg gtggcagaga
    39361 tccccgccag cagcgccata aagctgcccg ttgcgccaat cgcagtggtg gtaggggcgg
    39421 caaaattctc cgctttaaac cagaagatcc caaggccaat gacgatagtg aacgggataa
    39481 ttttggcgat ggtaattagc gtctgaaacg ctgcgccgcc ttcaacggag cgcaggtgta
    39541 gcaacataaa ggcgataatt aatccggcgg cgataaattt accgggcaac ggatcgatag
    39601 gcgttaaaaa gccaagattg ctgacaatcg ccagcgccat aatcgacaat gacggcgcat
    39661 cgttggccca gaagctggcc cagccggaga ggaaagccag cggtcggctt ccggcatttt
    39721 tcagataaac ataatctgcg ccattttccg gataagcggt ggatagttcc gcatagacgc
    39781 acatttgcgg gatcacaatt aacccgccaa tgacaaacgt gagcaccgta agccacggcg
    39841 tgcccgctgc ttttgccact tcacccacag atacaaaaat accggagccg acggttgtcc
    39901 cgacggcgat tgcaagaacg gcccaaaatc cgagcttgcg ttggagttcc tggcttccca
    39961 tagcgatacc tttttcttgt ccaacacgtt gtaggggaga gtttgcagtc atttaaaagg
    40021 taacatgaca atgcatgatg aatataataa catatgatgt tatgtaatgc ggaggaagat
    40081 cacaaaattt cgcacaggat cgcgctgtgg ctaatgaatg tagttatcca attgaaatta
    40141 aagtgaaaat atttttacgg gtggggagca agaaggatat agaaacaaat acgccctcgg
    40201 aaaatccaga gggcgtcggg caattaaacc ggtgttagcc gatttctgtc agagacttac
    40261 tgtgcagtag gaccgcaacc gccaatgatt ttagaaatag aaattgccgg gtgcagcagg
    40321 tagtcgtaag agcagttctt atcacggttt tcaatatgac cagtacatgc agttaacgta
    40381 gccagtactg cacctaccag tgcaacttta ataaatttgt tcatt
//
LOCUS       DADLFA010000012        40422 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000012 DADLFA010000000
VERSION     DADLFA010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40422
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.012"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(457..2148)
                     /gene="fruA"
                     /locus_tag="JQH65_000556"
     CDS             complement(457..2148)
                     /gene="fruA"
                     /locus_tag="JQH65_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HAY6138064.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAVARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(2165..3103)
                     /gene="fruK"
                     /locus_tag="JQH65_000557"
     CDS             complement(2165..3103)
                     /gene="fruK"
                     /locus_tag="JQH65_000557"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAY6138065.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(3103..4233)
                     /locus_tag="JQH65_000558"
     CDS             complement(3103..4233)
                     /locus_tag="JQH65_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HAY6138066.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFAVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            4601..5782
                     /gene="setB"
                     /locus_tag="JQH65_000559"
     CDS             4601..5782
                     /gene="setB"
                     /locus_tag="JQH65_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HAY6138067.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALTREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRTKD
                     V"
     gene            complement(5779..6033)
                     /locus_tag="JQH65_000560"
     CDS             complement(5779..6033)
                     /locus_tag="JQH65_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HAY6138068.1"
                     /translation="MECRPGCGACCTAPSIFSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAS"
     gene            6188..6760
                     /gene="yeiP"
                     /locus_tag="JQH65_000561"
     CDS             6188..6760
                     /gene="yeiP"
                     /locus_tag="JQH65_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HAY6138069.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            6983..8449
                     /locus_tag="JQH65_000562"
     CDS             6983..8449
                     /locus_tag="JQH65_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAY6138070.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLNE
                     QAPTLQIKDVDLTQYADKLIARFANPALKHKTWQIAMNGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            8567..9553
                     /locus_tag="JQH65_000563"
     CDS             8567..9553
                     /locus_tag="JQH65_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAY6138071.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWTVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            9592..10305
                     /gene="lpxT"
                     /locus_tag="JQH65_000564"
     CDS             9592..10305
                     /gene="lpxT"
                     /locus_tag="JQH65_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HAY6138072.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGLWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTNINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            10717..11283
                     /gene="mepS"
                     /locus_tag="JQH65_000565"
     CDS             10717..11283
                     /gene="mepS"
                     /locus_tag="JQH65_000565"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HAY6138073.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            11464..13020
                     /locus_tag="JQH65_000566"
     CDS             11464..13020
                     /locus_tag="JQH65_000566"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HAY6138074.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGGETPEQARWLSEPGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            13102..14916
                     /locus_tag="JQH65_000567"
     CDS             13102..14916
                     /locus_tag="JQH65_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY6138075.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADTLLNEAGWVLKGQQRVNATTGQQLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSHPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            14917..16011
                     /locus_tag="JQH65_000568"
     CDS             14917..16011
                     /locus_tag="JQH65_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HAY6138076.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     HNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTILTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            16011..17036
                     /locus_tag="JQH65_000569"
     CDS             16011..17036
                     /locus_tag="JQH65_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY6138077.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            17038..18627
                     /gene="yejF"
                     /locus_tag="JQH65_000570"
     CDS             17038..18627
                     /gene="yejF"
                     /locus_tag="JQH65_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HAY6138078.1"
                     /translation="MTQTLLAIEDLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFRGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNYAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKLQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(18631..18975)
                     /locus_tag="JQH65_000571"
     CDS             complement(18631..18975)
                     /locus_tag="JQH65_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6138079.1"
                     /translation="MTSLQLSIVHRLPQNFRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(19308..20491)
                     /gene="bcr"
                     /locus_tag="JQH65_000572"
                     /pseudo
     CDS             complement(19308..20491)
                     /gene="bcr"
                     /locus_tag="JQH65_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
     gene            complement(20519..21214)
                     /gene="rsuA"
                     /locus_tag="JQH65_000573"
     CDS             complement(20519..21214)
                     /gene="rsuA"
                     /locus_tag="JQH65_000573"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HAY6138080.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            21363..23123
                     /locus_tag="JQH65_000574"
     CDS             21363..23123
                     /locus_tag="JQH65_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAY6138081.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEAELNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            23248..23532
                     /gene="rplY"
                     /locus_tag="JQH65_000575"
     CDS             23248..23532
                     /gene="rplY"
                     /locus_tag="JQH65_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HAY6138082.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLAIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(23671..24678)
                     /gene="yejK"
                     /locus_tag="JQH65_000576"
     CDS             complement(23671..24678)
                     /gene="yejK"
                     /locus_tag="JQH65_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HAY6138083.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTKFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            24860..25087
                     /locus_tag="JQH65_000577"
     CDS             24860..25087
                     /locus_tag="JQH65_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HAY6138084.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            25107..26867
                     /gene="pbgA"
                     /locus_tag="JQH65_000578"
     CDS             25107..26867
                     /gene="pbgA"
                     /locus_tag="JQH65_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HAY6138085.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRPQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALLDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            26942..27018
                     /locus_tag="JQH65_000579"
     tRNA            26942..27018
                     /locus_tag="JQH65_000579"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26976..26978,aa:Pro,seq:ggg)
     gene            27237..28478
                     /locus_tag="JQH65_000580"
     CDS             27237..28478
                     /locus_tag="JQH65_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY6138086.1"
                     /translation="MARKTPPLTTVQIKAARPAEKEYTLQDGGGLFLLVKPSGSKLWR
                     FSYYRPSDKKRILLSFGSLDDVSLADARKRRSEYRALISAGTDPQGHEKKKREAEARR
                     QGNTFENVAAAWYQVKISQNLAPNTIKDIWRSLDKYVFPFIGNTPIDTLTARRFVEVL
                     TPIKERGNLETLKRVLQRVNEVMDYAANSGLIDANPAMNVRKAFPSPVKKHMPTIRPE
                     QLPELMQALSVSATERQTRLLIEWQLLTVTRPAEASSTRWDEINLDAKQWTIPAGRMK
                     MRRDHVIPLSGQAMAVLEAMKPISHHRNYVFPSLKDPQQPMNSQTANAALRRMGFAGV
                     LVSHGLRAIFSTAANEEGFEPDVIEAALAHVDTNEVRRAYNRSNYIEKRIVLMRWWGE
                     FVEAAETGVTLASGKRGIRAV"
     gene            28974..29180
                     /locus_tag="JQH65_000581"
     CDS             28974..29180
                     /locus_tag="JQH65_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HAY6138087.1"
                     /translation="MEAIRKIIFRQEVKKIIHIKADSTLQSMINAGEFPQGFRVGLRR
                     RGWYEDDVLAWQKEREQEARGTAA"
     gene            29195..29860
                     /locus_tag="JQH65_000582"
     CDS             29195..29860
                     /locus_tag="JQH65_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6138088.1"
                     /translation="MTNTKKAAPIWSRLSEQLTRCALCVYDPKHKHGDDSRYQAGGQC
                     NQSGSVRCHTCNERFSLYSLRNCSRAKAHGANLSDSCSIFLRRLFRAGDNVLVNSLSV
                     IVLSCIAMRRNTSHHGADSFYPLGSMPRRFSSVRPNSLSQLARLMPSLCASLSNCSFS
                     SGEIRILNCGDCPSPLGLLSRLIIDKWSPIELAFPLLGGHLNTRALKKAKPRKCHYHS
                     QGF"
     gene            29886..30086
                     /locus_tag="JQH65_000583"
     CDS             29886..30086
                     /locus_tag="JQH65_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HAY6138089.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            30076..30297
                     /locus_tag="JQH65_000584"
     CDS             30076..30297
                     /locus_tag="JQH65_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6138090.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            30290..30523
                     /locus_tag="JQH65_000585"
     CDS             30290..30523
                     /locus_tag="JQH65_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6138091.1"
                     /translation="MRDDRFNSLKQEFPGVPDDAADALSSMPELIRAAFFLLSTREYK
                     STGLDVLNIAADYAEYVAEARYRRKFPEDVSHA"
     gene            30516..30749
                     /locus_tag="JQH65_000586"
     CDS             30516..30749
                     /locus_tag="JQH65_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6138092.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            30755..31054
                     /locus_tag="JQH65_000587"
     CDS             30755..31054
                     /locus_tag="JQH65_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HAY6138093.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVMIKGVEERRVTY
                     RREGYEYDCVMPVYQFRRDFSLVQTAPHNVPTSNARARANIQKLKTMINGFRGKK"
     gene            31051..32451
                     /locus_tag="JQH65_000588"
     CDS             31051..32451
                     /locus_tag="JQH65_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAY6138094.1"
                     /translation="MKSAPNLKKQPYDKMTEVIIFAGSDAWAHAKQWQEQDGRLAGDN
                     VPPVVLADDQLDELADLRIIDEGRYCVRLYKAGHIRPSNINAIAHKLAAAGVTDANYY
                     PEGMHSHMRENWREYLERVRGKEPVEEKNHQRKTTLPMSVGSTGYDTQLDYVVKGIIP
                     AVSLCSIYGASGSYKSFLAGSWACHVATGRQWGGRRVAHGAVLYVVGEGGIGVPRRVK
                     AWEVVHDEQVKNLYLVNRPIFPAAPLDVDEMVIAARQVERETGKPVRMIILDTLARCF
                     GGNDENDSRDMGAFIRGCDELKRRTGATVLVVHHSGKDETKGARGSSAFRASLDAEYR
                     IRREDAGSKALVISCTKMKDAEELKEAAYDLRVVELFTDADGELITSLVVVDDPRPPV
                     ELERIEEAGNKTENHTALWGCIRSRTQNGDKCTIPLLRDDMKKLGYEMKNFRRWLYKL
                     EKDGVISIDGDDVAPL"
     gene            32653..32904
                     /locus_tag="JQH65_000589"
     CDS             32653..32904
                     /locus_tag="JQH65_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6138095.1"
                     /translation="MTQKRRDRTEPKYKALDMTEHTLKVAIRTIDRHTREGYAKEHPD
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKTGEVEL"
     gene            32901..33311
                     /locus_tag="JQH65_000590"
     CDS             32901..33311
                     /locus_tag="JQH65_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAY6138096.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTPMTLARMAVSLPCSQAQDGQ
                     ATLWLSVIAFGKQADFLAKHQKGDVASVSGTMQVSQWTGQNGETRQGWQVIADSVISA
                     RAARPGGNRRKTTGTQGNQPPAGGDDPYGDDISF"
     gene            33322..33594
                     /locus_tag="JQH65_000591"
     CDS             33322..33594
                     /locus_tag="JQH65_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HAY6138097.1"
                     /translation="MVHDRIAEELEAKGFYRRAAARWGEVMQLVETDKERHHITMRRL
                     ECSRKAQRAPEPPDNFGDLKKAVDRTYAEMGIDGAGDEIWRNYQDS"
     gene            33720..33944
                     /locus_tag="JQH65_000592"
     CDS             33720..33944
                     /locus_tag="JQH65_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6138098.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMSINDYRAFLETGALIEKDQHG
                     FVRCALSGEMLAVNPEQIDALIEFLKEIRD"
     gene            34196..34402
                     /locus_tag="JQH65_000593"
     CDS             34196..34402
                     /locus_tag="JQH65_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6138099.1"
                     /translation="MAEEKKGGVSVYISPEIVEVLKQRHKKNYEAGVAAGLDPLMTPE
                     PSIGSLVRSYLLAALGMHKNYGGE"
     gene            34402..35457
                     /locus_tag="JQH65_000594"
     CDS             34402..35457
                     /locus_tag="JQH65_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HAY6138100.1"
                     /translation="MAGKATALNTNQLFMYLNRGDIADFKFSPLFTTLFFPNVATFST
                     QNIMLDTLDIEEVTMSAFCSPMVGSQVQRDKGYETSTIKPGYMKPKHEIDPTKTIMRM
                     AGEDPAQLNDPTYRRMRLITGNMRRQINAIKARVEWLAVNAVTTGKNIIEGEGIERYE
                     IDWKIPEKNIIEQSDGKKWSEHDKDIYDPIYDIELYADQAGCPANVMIMGAEVWRTLR
                     SFKKFRELYDLSRGSESAAEMACKNLGEVVSFKGYLGDIALIVYSGKYTDSDGTEKYF
                     LEPDLLVLGNTNNKGLVAYGAIMDQEAVRTGATQNMYYPKNWIEDGDPAIEYVQTHSA
                     PQPVPADIRKFVTVKIG"
     gene            35469..35804
                     /locus_tag="JQH65_000595"
     CDS             35469..35804
                     /locus_tag="JQH65_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HAY6138101.1"
                     /translation="MDTPYIELFAGSQQVSTTLVHFAADAGVIQEFTPLMLVDNGEFK
                     PWDGQESGKAVYLTSYLVDTSKQKSAQCYKTGIFNIAAVNWPESVDTDAKKCAAFAGS
                     GVSVQPLAR"
     gene            35817..36230
                     /locus_tag="JQH65_000596"
     CDS             35817..36230
                     /locus_tag="JQH65_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HAY6138102.1"
                     /translation="MATNESIMALPLASKFKAEARAMADRGLSTYEAVYQLNKLEEQD
                     KPRADAIMALHEHKDYQPLLRAMANVPCIDVDTAKNILNMTIEQERPKVAPELTAAFE
                     NFMDMHSPQTVSAGMAYDGRNPGDDGDIDRILKTI"
     gene            36392..36826
                     /locus_tag="JQH65_000597"
     CDS             36392..36826
                     /locus_tag="JQH65_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6138103.1"
                     /translation="MIDKACFVSQQEIAEHFKVNRTTIRAWTKQGMPYLNADRGKSGG
                     YHIGHTLLWSSGKSRLEAIRYHVETSALEKIMFARLLSSERDEYSSEETEHRFDEGLQ
                     IYGYSPEDVSKARNKMAGFLAGWRHAVSVRRASMEQSADTEQ"
     gene            37107..37388
                     /locus_tag="JQH65_000598"
     CDS             37107..37388
                     /locus_tag="JQH65_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6138104.1"
                     /translation="MTEAEILGLIRRAGGISQQTDEQATQPDSVTAENYARVVAEVMR
                     RDGIQLNDVDMRNIRIRVLEMLAYNRRVALYRETEKITYHWKKPERLRR"
     gene            complement(38066..>40422)
                     /gene="yejO"
                     /locus_tag="JQH65_000599"
     CDS             complement(38066..>40422)
                     /gene="yejO"
                     /locus_tag="JQH65_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HAY6138105.1"
                     /translation="GGYQYIEMNGTAEYSVLNDGYQIVQMGGAANQTTLNNGVLQVYG
                     AANDPTIKGGRLIVEKDGITVLAAIEKGGLLEVKEGGLAIAVDQKAGGAIKASTRVME
                     AFGINRLGQFEIKNGIANNMLLENGGSLRVEENDFAYNTTVDSGGLLEVMDGGTATGV
                     DKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYELDDGSGLIVMEDTQAIDTILD
                     EHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSSKAISENMVINNGRANVWAGTM
                     VNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGASFRTHGAVDTSKADVSLENSVW
                     AIIADITTTNQNTLLNLANLAMSDANVIMMDEPVTRSSVTASAENFITLTTNTLSGNG
                     NFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASPAAGDSLTLVTTGGGDAAFTLG
                     NAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTTDVLNMAAAQPLVFDAELDTVR
                     ERLGSVKSVSYDTAMWSSAINTRNNVTTDAGAGFEQTLTGLTLGIDSRFSREESSTIR
                     GLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNGAYVDGVVKVDRFANTIHGKMS
                     NGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAFTGFTTDGQDYTLSNGMRADVG
                     NTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEYADSNHVKVNDDGKFNNDVAGT
                     PGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQAGVVWTF"
BASE COUNT        10206 a        10258 c        10107 g         9851 t
ORIGIN      
        1 agcctataat tacgacataa tccttttcgc gcatagcaca tcctctggta aaaaattacc
       61 aaccctgtca atccacaaga atatagatac tgcaaagacg gtttaaacat gtgttcatat
      121 ttaaacatgt gctcatagca tgcatgtttt acgtaaagta aattaccgcc agagaggcaa
      181 aatgggcaga tttgagcagg ctcacaaaat gcgattaaat gcctgaatta caaaagcgag
      241 gaatttgtga cgggatgcac agaacgggaa caataaaaaa ggcagaaaat aatctgccct
      301 tgaggaaata accttagtgt ttgatgaacg tttcaagttt ctactgaaag ataaaaccat
      361 ttaaattcat aaaattacat tcacaaaccg gtcattctct ttgcgctcat caaatgttac
      421 agggcaggaa atttctgccc tgtaacacac cttttattac gctgctttcg ctactgcgtc
      481 cacttccgga cgtttcagga aggcataggc caaacccgcc accagcgtac cggcaataat
      541 tgctaccagg taacccagta ccggcgtaat agcgccaggg atcagcagaa caaacagacc
      601 accgtgcggt gccatcagtt tcgcaccaat cgccattgag attgcgccag tcagcgcccc
      661 acccacgata cagcacggca gcacacgcat cggatcacga gcagcaaacg gaattgcacc
      721 ttccgaaatg aagcacagtc ccaataccag agcggctttg ccaccttcct gctgcgcttt
      781 gtcgaatttg cgacgcgcca ccattgttgc cagacccatt gccagcggtg gcaccatacc
      841 tgccgccata atcgccgcca tcgggccata ggtttgagta ctcagcagac ccacaccgaa
      901 tgcgtacgct gctttgttta ccggaccgcc catgtcagta cacatcatgc caccgaggat
      961 cgcccccagc agaaccgcat tcgcagtccc catggtctgc agccagtgag tcagcccttc
     1021 gagaatgcca gcaactggtt taccgatcag gtagatcatc gccagaccga ccaccagact
     1081 ggaaattagc ggaatgatca ggatcggttt cagcgcctcc atactctgtg gcagtttcag
     1141 ttgcgtactg attaacttcg caatgtaacc agccaggaag ccagcaatta taccgccaat
     1201 aaagccagaa ccggtgctga ccgccagcat accgccaatc agacccggcg tgaggcccgg
     1261 acgatcggca atggagaagg caatataacc tgccagtacc ggcaccatca gcgcaaaggc
     1321 tgaaccaccg ccaatctgca tcagcgccgc tgccaacgta cccggctctt taaacgcttc
     1381 gataccaaaa gcaaaagaaa gcgcgataca cagaccacct gcgacgacca tcggcagcat
     1441 ataagagacg cctgtcagca agtgacggta agcgcctgca ctctctttct taccttcagt
     1501 ggtcgccgtt tgagctttgc ccgccggttc atacggcgtt gcttcagcaa ccgctttatc
     1561 cagttcctgc gcggttttct tcagcgccag accggtagag gtacgataca tcggtttacc
     1621 agcaaatttc gccagatcca cttcgatatc tgccgccaca atcaccagat ccgctgctgc
     1681 gacttcttca ggggtgattg cattgcccgc gccaacagaa ccacgggttt caactttcac
     1741 ccaccagcca cgttttttcg cttcggtttc aatggcttca gccgccataa aggtgtgtgc
     1801 tacgccagtc gggcaagcag tcaccgcaac tacgcgtttc ggaccgctgg cagcaaccgg
     1861 tgctgtcgca gcgaccggcg cagtgtaagg tttcgcatgg cctttggctt cactcaggaa
     1921 cagctcaggg tgcgcaactg cccgggaaat atcacccagc cagacatttt taccgttcag
     1981 cgcgctgtca ttcgggatgg aatcaccgag aacaatcgcc atttctgcgt cgttcggatt
     2041 gtcgatgatt tccagttttg cttttcgcgc caccgcgccc agcagggtct tcgccatata
     2101 ggcgcgtgcc tgaccgagat tagcgtcaat aatcagcagc gttttcatta tgcctctcct
     2161 gctgtcagtt aaaaggttgt aagtcgacgc gcgccatcat tgcggccaac tgcggacgat
     2221 cggtaatacc cacattgctt tgacttaccg ccagggctgc aacagctgtc gccagacgca
     2281 gtgtgtgttc actggattca cgcatcagca agccataaat caggccacca accatagaat
     2341 cccctgcgcc aacggtgctt acgacatcga ctgacggtgg tttggcgatc cattcgccgg
     2401 aggcattaac ccaaagcgcg ccttcggcac ccagtgaaat aacaacatgc gcaatacctt
     2461 gttcacgtag cgcatgtgca gcttcaatca catctttcat ttcaggcagt ttacggcctg
     2521 cccaaatttc caactcgcgg cggttaggtt tcaccagcca cggtgccgct ttcaaacctg
     2581 ctactaacgc ttcacggcta ctatcaaaga taatgcaagg acactgacta cgcaggcgag
     2641 tcatccagtc ggtgaacgct tccgggctga cgcctgacgg taagcttccg ctgacacaga
     2701 ccatatcgaa ctgaccgagc cagctcagag aatcagtcac aaagcgttcc cagtcggcgg
     2761 gggtgacttc aaaacccgag aagttgaagt cggtcacttc gccgtctttt tccgtcagct
     2821 taacgttaat tcgggtgcgc ccctgtacaa cctggaaacg gttggcaatg cccagctcgc
     2881 tgaacagttg ctgaaaacca tcctgattgt ctttacccag gaagccgcca acggtgacat
     2941 caattcccag gtcttttaat actttggcca cgttgatgcc tttacccgcc gcatgcagac
     3001 cggtggtttt caccaggttc acttcgccgc gttcaatttc cgggcagaaa ccaacaaggt
     3061 cataagccgg attaagggtg atcgtagcaa cacgtctgct cattatgcgc cctccccaag
     3121 accagcagcg atagcgtcgc caatggcttt cagcgcctgt tcagcatctg caccctgggc
     3181 ggtaaagcgt aggcgatgac ctttcttaac gccaagtgcc acaactttca tcagactacg
     3241 tccgtttgcc ggtttgccgg tgccatcaag gtttgtcacg gtaatatcac tgttaaattg
     3301 tttaatggta ttgaccagca tggtacctgg acgagcatgc aggccgtgtt cattgcgcac
     3361 cacaaactcc gcgcttaaca cgtcgtcggt cggcgcatca tcgctggtca gcagcgccag
     3421 caacgttgcc gcatctgctt tcagcaagcg gtcagcttta ttgtcgagca ataaatcagc
     3481 gagacgctta agaaccgcga tgggctgatc gtcattcatc gccacactca ccagcatggc
     3541 tgccgtttcg ccgtccacag caaaagcatt tgccgcacgg cttaccgcaa tcgcgctacg
     3601 cagattgcct tcggcgctat cgctcagcca gataccctgt ccgagattca gcggttgttc
     3661 attgatggct ttggtgacga aagtggcgtc aactgccccc gcctctttca gacgcgcagc
     3721 gttcagcgcc tgaagagtca gcagatcgct ggcgacgata tccagcgtca gcatttcgtt
     3781 gtcgagcttc agctgctcac tctgcttttc gcccatcagt aatgcgcgaa gttcttctgc
     3841 tgttgttgct gacttcagtt gttcagcaac ggaatcatcg ctcagtacgt gggtcagctg
     3901 gcgtagcagg cccagatgtt catccgagct ggcagcaata ccgattgcca cgtacgctac
     3961 ctgaccgtca ccccaggtga cgccttccgg gaactgaaat acctgaacgc cggttttcag
     4021 cacctgatcg cgggtgtcgg tagtgccgtg tggaatagca ataccattgc cgaggaacgt
     4081 tgaggtttgc tgttcgcgcg ccagcatgcc attgacgtag ccttctgcta cattaccggc
     4141 ctgcaccagc gccgcagcga cctggcgaat cgcctcttct ttgtctccgg ccttttcgcc
     4201 cggatggatg tcctgtacgg ataactggaa catagttctc ctctcttgct gaattgaaac
     4261 gattcagcct ctatgagaaa aaaagcgcca acctggctta gggttaaaga caagatcgcg
     4321 ctgaaacgtt tcaagaaagc ataatacttc tgtttcagca cgcaaggaaa gccgtaaagt
     4381 tatgagcaaa agtttgatgt gctgcacatt tttttgtcat ttttcctaat tgctgatggg
     4441 aaaatccgtt atcagcaatt tcatttcagc agcgtcagca gccaatcagc aacagtgaaa
     4501 tggcattttg attattcgtg gcgcttttga tttcgctgtc tgtttatttt ctgacaagca
     4561 gcgtaaactc cgcgtcttcc tcttccagtg atagacctgc atgcataact cccccgcagt
     4621 ctccagcgcg aaatcgtttg acctgacctc gacggcgttt ttaatcgttg cctttctcac
     4681 cggtattgcg ggtgctctgc aaaccccgac actcagtatt ttccttaccg atgaagtaca
     4741 tgcccgtccg gcgatggtgg gattcttctt taccggcagc gctgtcattg ggattctggt
     4801 aagtcagttt ctcgctgggc gctctgataa gcgcggcgat cgcaaatcgc tgattgtctt
     4861 ttgctgcctg ttaggcgtgc tggcctgcac cctttttgcc tggaatcgca actactttgt
     4921 tttgctattc gttggcgtct ttcttagcag ctttggctcg accgctaacc cgcaaatgtt
     4981 tgcccttacc cgtgaacatg ccgacaaaac cggacgtgag gcggtgatgt tcagctcttt
     5041 tttacgcgct caggtttcac tggcatgggt cattggccca ccgctggctt atgcgttagc
     5101 gatgggtttc agctttacgg taatgtatct gagcgcagcg gtagcgttta ttgtttgcgg
     5161 cgtgatggtg tggctgtttt taccgtcgat gcaaaaagag cttccgctgg cgaccggcac
     5221 ggttgaagcg ccgcgccgta accgtcgcga tacgctactg ctgtttgtca tttgtacatt
     5281 gatgtggggc tcgaacagcc tgtacatcat caacatgccg ctatttatta tcaacgaact
     5341 gcatcttccc gagaaactgg ccggtgtgat gatggggacc gccgccgggc tggaaatccc
     5401 gacgatgttg attgccggat atttcgccaa acgtctgggt aagcgtttct taatgcgcgt
     5461 tgctgccgtg ggtggcgtct gtttttacgc aggaatgctg atggcgcatt cacctgccat
     5521 tctgttgggc ttgcagctgc taaatgctat ttttattggc attctgggcg gcatcgggat
     5581 gctctatttt caggatttga tgcccggtca ggcaggttca gccaccacgc tctataccaa
     5641 cacgtcgcgc gtgggctgga tcatcgcagg atcagtggcg ggcatcgtcg ccgagatctg
     5701 gaattatcac gctgtgttct ggtttgcgat ggtgatgatt atcgccactc tgttttgctt
     5761 actgcggact aaagatgttt aagacgcggt cagcatttcc agatcgataa gccaggtcat
     5821 ggcctgctga cgcgagttac cgcacatttc agcgctggct tgtaagcctg cacagacttt
     5881 cggtcgcagt ggcgaggtga aaattttaca gcgttgctgt tcatcaagct gaatgcaggg
     5941 cgtattggcg ggcttgccat ctggcatacc gggaataggg ctgaaaattg aaggggcagt
     6001 gcaacacgcc ccgcaacccg gacggcattc catacgttgt tctcttaagg ttaaccaccg
     6061 gattgatgag cgcgcacagt accacctttt ttgcaccagc aaaagtgcga ataccacttg
     6121 cccgaaaggc ccgtcgcgag tactttgtcg cgatattttt gacattttcg actacaggaa
     6181 tttttcgatg ccaagagcga acgaaattaa aaaaggtatg gtactgaatt acaacggcaa
     6241 actgctgctg gttaaggata ttgatattca gtcgcccact gcccgcggtg ccgctacgct
     6301 gtacaaaatg cgtttttctg atgtccgtac cgggctgaaa gtagaagagc gtttcaaagg
     6361 tgatgatatc gttgacaccg tgacgctgac ccgccgttac gttgacttct cctatgtcga
     6421 tggcaacgaa tatgtcttta tggataaaga agactatacc ccgtatacct tcaccaaaga
     6481 tcagattgaa gaagagttgc tgtttatgcc agagggcggc atgccggaca tgcaggtgct
     6541 gacctgggac ggtcaactgc tggcgcttga gctgccgcag accgttgatc tggaaatcgt
     6601 tgaaaccgca ccaggcatca aaggggcatc tgccagcgcc cgtaacaaac cggcgacatt
     6661 gagcactggt ctggtgattc aggtaccaga atacttaagc ccgggcgaaa aaattcgtat
     6721 ccatatcgaa gaacgccgtt atatggggcg tgctgactaa cttcagccgc atgcagaaaa
     6781 gggatagctc aggctgcccc ttttttaatt tattatacca atcttctatt ttgcgcttcc
     6841 tgaacacctt acgccctcaa ttttcactcg ttgattgatc gccctcacac ttcatcgcat
     6901 taacaatcca gaccaatttc aattgctgtc atataacttt acactgtcat tgttaattta
     6961 tcgttactaa gacgtgactc ctatgaagac aattgcctcc gttacgctcc cgcatcatgt
     7021 acacgctcca cgctacgatc gccagcagtt gcaatcacgt atcgttcatt ttggctttgg
     7081 cgcctttcac cgcgctcatc aggcgttact gaccgatcgt gtgctgaatg cccagggcgg
     7141 cgactggggg atctgtgaaa tcagcttgtt cagcggtgat caactgatga gccagctccg
     7201 cgcacagaac catttatata ccgtgctgga gaaaggtgcg gacggcaatc aggtgataat
     7261 tgtcggtgcc gttcacgaat gccttaatgc aaaactggat tccttagcgg caattattga
     7321 gaaattttgc gagccacagg tggcaattgt ttccctgacg attaccgaaa aaggctattg
     7381 tattgacccg gccaccggtg cactcgacac cagtaatccg cggattattc acgatctaca
     7441 aacccctgaa gaacctcact ccgcaccggg tattctcgtc gaagcactga aacgccgccg
     7501 tgagcgcggc cttacaccgt ttaccgtgct ctcctgcgac aatattcccg acaatggcca
     7561 tgtggtgaaa aacgcggtgc tgggaatggc agaaaaacgt tcgccagaac tcgccgggtg
     7621 gataaaagaa cacgtcagtt ttccgggaac catggtcgac cgcattgttc cggctgcaac
     7681 cgacgaatca ctggcagaaa tcagccagca tctgggggta aatgatccct gcgcgattag
     7741 ctgcgaaccg tttatccagt gggtggtgga agataacttc gtcgctgggc gtcctgcctg
     7801 ggaagtcgca ggtgtacaaa tggtgaatga tgtcctgcca tgggaagaga tgaaactgcg
     7861 tatgcttaat ggcagccact cttttctcgc ttatctgggt tacctttcag gattcgccca
     7921 tatcagtgat tgcatgcagg atcgcgcatt tcgccatgcc gccagaacat taatgctgaa
     7981 tgagcaagcg ccgacactac aaattaaaga tgtcgattta acacaatatg cggacaagtt
     8041 aattgctcgt tttgctaatc cggcgctgaa acataagacc tggcaaatcg caatgaatgg
     8101 tagccagaaa ttaccgcaac gtatgctggc aggtattcgc atacatctgg ggcgcgaaac
     8161 ggactggtcg ttgctggcat taggcgttgc tggctggatg cgttacgtca gcggcgttga
     8221 tgatgccgga aatgccattg atgttcgcga tccgcttagc gataaaattc gcgaacttgt
     8281 tgcgggcagc agcagtgaac aacgcgtaac cgccctgctt tccctgcgtg aagttttcgg
     8341 tgatgatctg ccagataacc cgcattttgt gcaggccatc gaacaagcct ggcaacaaat
     8401 cgtacaattc ggcgcacatc aggcgctatt aaacaccctc aaaatttaac gatttctgcg
     8461 gttaaagcgg atgaagctca ccttcgtccg ctctcccctt ctcttttctg ccttttttag
     8521 ccaggattaa cgctcagtta acttaccaga gtattgcgga gccgttgtga ccaggaccaa
     8581 cctcatcacc ggttttctcg gcagcgggaa aaccacgtcg attcttcatc tgttagccca
     8641 taaagatccc aacgaaaaat ggacggtact ggttaatgaa tttggggaag tcggaattga
     8701 tggtgctttg ctcgccgata gcggcgcatt gctgaaagag atccccggcg gctgcatgtg
     8761 ctgcgttaat ggtttaccca tgcaggtagg gttgaatacc ttactgcgtc agggaaaacc
     8821 agaccgcttg ttgatagagc cgaccgggct gggccatccg aaacagatcc tcgatctgtt
     8881 aaccgcacca gtctatgaac cgtggataga tctgcgcgcc accttgtgca ttctcgatcc
     8941 acgcttgctg ctggacgaaa aaagcgccag caatgaaaac ttccgtgacc agctggctgc
     9001 cgcagacatc attgtcgcca ataaatccga ccgtgcgacg cccgaaagtg agcaagcgct
     9061 acagcgttgg tggcagcaaa atggtggcga tcgacaatta attcacagtg agcatgggaa
     9121 agttgacggt catcttctgg atttgccgcg tcgcaattta gccgagttgc ccgccagcgc
     9181 cgcgcattct catcagcatg tcgtgaaaaa agggttagca gcgttaagcc tgccagagca
     9241 tcaacgctgg cgtcgcagtc tgaacagcgg gcaaggatat caggcctgcg gctggatatt
     9301 cgacgctgat acggtattcg acaccattgg cattctggaa tgggcgcgac ttgcaccggt
     9361 ggaacgcgtc aaaggcgtgc tgcgtattcc cgaagggctg gtacgaatca accgtcaggg
     9421 cgatgacctg cacattgaaa cgcaaaacgt tgcgccaccg gacagccgta ttgagctgat
     9481 ttccagcagc gaagctgact ggaatgcctt gcagagcgcg ctgttgaagc ttcgtttagc
     9541 gactaccgcg taaggttgcc tgcgtttttc agtaagataa ttagagaaaa tatgattaaa
     9601 aatttgccgc aaatagtgtt gttgaatatt gtcggcctcg cgctgtttct ttcctggtat
     9661 attcccgtta atcatggatt atggttgccg attgatgcgg atatttttta tttctttaat
     9721 cagaaactgg tcgaaagtaa ggccttttta tggctggttg cattgaccaa caatcgcgct
     9781 ttcgacggtt gttctctgct ggcgatggga atgttgatgc tgagtttctg gctgaaagaa
     9841 aacgccccag gcagacgacg tatcgtgatt attggtctgg taatgctatt aactgcagtg
     9901 gtattaaacc agctgggtca ggcattaatt cctgtaaaac gggccagccc aacattgacg
     9961 tttactaata ttaaccgcgt cagcgaactg ctctctgtcc ccacgaaaga tgcctcacga
    10021 gatagctttc ctggcgatca cggcatgatg ctgcttattt tttcggcatt catgtggcgt
    10081 tatttcggta aagttgcagg tcttatcgcc cttattattt ttgtggtttt tgcgtttccc
    10141 agagtaatga ttggcgcaca ctggtttact gacatcattg tcggttcgat gaccgtgata
    10201 ttgatcggtt tgccctgggt gttgctgacg ccattaagtg atcgattaat cacctttttt
    10261 gacaaatcac taccgggaaa aaacaaacat ttccaaaaca aataactcac agtaattaac
    10321 atcatcaggt ttatttttat agtgaggata atcctgatga tgcgcaccgt gctttcatct
    10381 atcgaacgca aaaatcattc tctaagtaaa tgaatggatt gcatgcgttt cactcaatcg
    10441 tactttaatt gaccaatccc gcttattaac tttctgtatc actttttctt ataaaaaatc
    10501 atgtaaaacc gctcgccaag accgcaccaa tcgggtaatc tcgaactcgt tttgcctcgg
    10561 cggtagatta tcctcacagc atataatttt gtgcgttagt ccacagattt ggccttaagg
    10621 aattgtttca acatgcccag gtaattagtc tcgtgtcgct tggcattttt ttataacgat
    10681 atttgtcgtt aaggacttca agggaaaaca aacaacatgg tcaaatctca accgattttg
    10741 agatatatct tgcgcgggat tcccgcgatt gcagtagcgg ttctgctttc tgcatgtagt
    10801 gcaaataaca ccgcaaagaa tatgcatcct gagacacgtg cagtgggtag tgaaacatca
    10861 tcactgcaag cttctcagga tgaatttgaa aacctggttc gtaatgtcga cgtaaaatcg
    10921 cgaattatgg atcagtatgc tgactggaaa ggcgtacgtt atcgtctggg cggcagcact
    10981 aaaaaaggta tcgattgttc tggtttcgta cagcgtacat tccgtgagca atttggctta
    11041 gaacttccgc gttcgactta cgaacagcag gaaatgggta aatctgtttc ccgcagtaat
    11101 ttgcgtacgg gtgatttagt tctgttccgt gccggttcaa cgggacgcca tgtcggtatt
    11161 tatatcggca acaatcagtt tgtccatgct tccaccagca gtggtgttat tatttccagc
    11221 atgaatgaac cgtactggaa gaagcgttac aacgaagcac gccgagttct cagccgcagc
    11281 taataaaccg tttggatgca atcccttggc tatcctgacg agttaactga aagcactgct
    11341 taggtagtgc ttttttgttt tcattcatca gagaaaatga tgtttccgcg tcttgatcca
    11401 ggctatagtc cggtcattgt tatcttttaa atgttgtcgt aatttcagga aattaacgga
    11461 atcatgttca tacgcgctcc caattctgga cgtaagctcc tgcttacctg cattgtagca
    11521 ggcgtgatga ttgcgatact ggttagctgc cttcagtttt tagtggcctg gcataagcac
    11581 gaagtcaaat acgacacact gattatcgac gtacaaaagt atctcgatac ctattttgcc
    11641 gacctgaaat ccactactga ccggcttcag ccgctgacct tagatacctg ccagcaggct
    11701 aaccccgaac tgaccgctcg cgcggcgttt agcatgaatg tccgtacgtt tgtgctggtg
    11761 aaagataaaa aaacattctg ttcatctgcg actggtgaga tggacattcc actcaatgaa
    11821 ttgattccgg cgctcgacat taataaaaac gtcgatatgg cgatcttacc cggcacgccg
    11881 atggtgccga acaaacccgc aatcgtcatc tggtatcgca accctttgct gaaaaatagc
    11941 ggtgtgtttg ccgctctgaa tctcaacctg acgccttctc tcttttatag ttcacggcag
    12001 gaagattacg atggcgtcgc cctcattatt ggcaatactg cgctatctac cttttcttca
    12061 cgtttgatga acgttaatga attaaccgac atgcctgtcc gtgaaactaa aattgcgggc
    12121 attcctctga ccgttcggct ttatgcggat gactggacat ggaacgatgt gtggtacgca
    12181 tttttactgg gcggcatgag tggaactgtc gttggcctgc tctgctatta cctgatgagc
    12241 gtacgtatgc gccccggcag agaaatcatg accgccatca agcgcgaaca attttacgtg
    12301 gcgtatcaac cggtggtgga tacacaagct ttgcgagtaa cgggcctgga agtactgcta
    12361 cgctggcggc atcctgtcgc gggagaaatt cccccggatg ccttcattaa ctttgccgaa
    12421 tcgcaaaaga tgattgtgcc gctgactcag cacctgtttg agttaattgc ccgcgatgcc
    12481 gcagaattag aaaaagtgct gccagtaggc gtcaaatttg gtattaacat tgcgccggac
    12541 catctgcaca gcgaaagctt taaagcagat atccagaaac tgctcacttc cctgcccgca
    12601 caccatttcc agattgtgct ggaaattacc gagcgcgata tgctgaaaga gcaagaagcc
    12661 acacaactct tcgcctggct gcactcggtc ggcgtagaaa ttgctattga tgacttcggc
    12721 accgggcaca gcgcgcttat ctatcttgag cgttttacgc tcgattatct gaaaattgac
    12781 cgtggattta tcaacgccat cggtacggaa acgatcactt cacccgtact tgacgcggtg
    12841 ctgacgctgg cgaaacgcct caatatgctg acggttgctg aagggggcga aacgccggaa
    12901 caggcgcgat ggctaagcga acccggcgtt aatttcatgc aaggctactg gattagtcgc
    12961 ccgttaccgc tggacgattt tgttcgctgg ctgaagaaac cgtatacgcc gcagtggtaa
    13021 ggtgtgctta cgtcccttat tattcatagt gaaagcatgc cggattgcgg ctaatgatga
    13081 gtaaaaggaa atccgttgca gatgattgtg cgcatactgc tgctgtttat cgccctgttc
    13141 accttcggtg cgcaggcgca ggctatcaag gaaagctatg cctttgccgt gctgggcgaa
    13201 ccccggtacg catttaattt caaccatttt gattatgtga accccgccgc gccaaaaggt
    13261 gggcagataa cgttgtcagc cctcggtacc ttcgataatt tcaaccgcta tgcactgcgc
    13321 ggcaacccgg gcgcacgcac cgagcagttg tacgacacgc tatttacgac ttccgatgac
    13381 gaaccaggca gttattaccc gctgattgct gaaagtgcac gctatgctga cgattattcc
    13441 tgggtggagg tcgctattaa tccgcgcgcc cgttttcatg atggttcgcc cattactgcc
    13501 cgcgatgtag agtttacttt tcaaaaattt atgaccgaag gcgtgccgca atttcgtctg
    13561 gtctacaaag gcaccaccgt caaagccatt gcaccgttaa ccgtgcgcat tgagttagct
    13621 aaacccggca aagaagatat gctgagtctg ttttcgctgc cggtatttcc agaaaagtac
    13681 tggaaggatc acaaacttag cgacccgctc gccacgcctc cgcttgccag tggtccgtac
    13741 cgcgttacgt cctggaaaat ggggcaaaat attgtctatt cccgtgtgaa agattactgg
    13801 gcagcaaact taccggtaaa ccgtggacgc tggaatttcg acaccattcg ctacgattat
    13861 tacctcgatg ataatgtcgc ctttgaagcg tttaaagcag gtgcctttga tttgcgtatg
    13921 gaaaacgacg ctaaaaactg ggccacgcgt tataccggta aaaatttcga taaaaaatac
    13981 atcatcaaag atgagcaaaa gaacgaatca gcccaggata cgcgctggct ggcgtttaat
    14041 atccaacgtc cggtattcag cgatcgccgg gtccgggaag caatcactct cgcctttgac
    14101 tttgaatgga tgaacaaggc gttgttttac aacgcctgga gtcgcacgaa cagttatttt
    14161 cagaataccg aatacgcggc cagaaattac cccgacgccg cggagctggt gcttctggca
    14221 ccaatgaaaa aagatctacc gccagaagtc ttcacacaaa tctaccagcc gccggtatct
    14281 aaaggcgatg gctacgatcg tgacaacctg ttaaaagccg acacacttct caacgaagcg
    14341 ggctgggtgc tgaagggtca gcaacgcgtt aatgccacaa cgggtcagca actcagcttt
    14401 gaattattgc ttcccgcaag cagcaatagt cagtgggtat tgccgttcca gcacagcctg
    14461 caacggctgg gtatcaacat ggacattcgc aaggtggata actcgcaaat caccaaccgc
    14521 atgcgcagtc gcgactatga catgatgccg cgcctatggc gggcgatgcc gtggcccagt
    14581 tccgatttac agatttcctg gtcatcggaa tatatcaatt ccacttataa tgcccccggc
    14641 gtgcaaagcc cggttatcga ctcactgatc aaccaaatta ttgccgcgca gggaaataaa
    14701 gaaaaattat tgccgttggg gcgagcactg gatcgcgtat taacgtggaa ttattacatg
    14761 ctgccaatgt ggtacatggc ggaagaccgt ctcgcctggt gggataaatt ctcccacccc
    14821 gctgtacgcc ctgtttacag cctgggtatc gatacctggt ggtatgacgt taataaagcg
    14881 gccaaactgc cgtcagccag gcaacaggga gagtagatgg gcgcttacct gattcgccgt
    14941 ctgttgctgg tgatcccaac attatgggcg attatcacca tcaacttttt catcgtgcaa
    15001 attgcgcctg gcggtccggt cgaccaggcc atcgccgcca ttgagtttgg taatgccgga
    15061 gtattacccg gcgcaggcgg tgaaggtgtt cgtgccagcc atgcgcaaac gggtgtcggc
    15121 aatatcagcg acagtaatta ccgtggcgga cgcggattag atccagaagt gatcgctgag
    15181 atcactcatc gctacggttt tgataagccg atccacgaac gttacttcaa aatgctctgg
    15241 gactacatcc gctttgattt tggtgatagc ctgtttcgca gcgcctcggt gctgacgctg
    15301 attaaagaca gtctgccggt ttccatcacc ctcggattgt ggagcacgct gattatctat
    15361 ctggtgtcga ttccgttagg cattcgcaaa gctgttcata atgggagccg ctttgacgtc
    15421 tggagtagcg catttatcat tatcggctac gccattccgg cctttttgtt tgccatcctg
    15481 ctgattgtct tcttcgcggg cggcagctat ttcgacctgt tccctctacg cggcctggtt
    15541 tccgctaact ttgattcgct gccgtggtat cagaaaatca ccgattatct gtggcatatc
    15601 acgctgccgg tgctggcgac ggtgattggt ggctttgcgg cgctgaccat actgacaaaa
    15661 aactcattcc ttgatgaagt gcgcaagcaa tacgtggtga ccgcgcgtgc gaaaggggta
    15721 agtgaaaaaa atattctctg gaaacatgtg ttccgcaacg ccatgctgct ggtgattgcc
    15781 ggttttccgg cgacgtttat cagcatgttt tttaccggct cgctgctgat tgaggtgatg
    15841 ttttcactca atggtctggg cttactgggc tacgaagcga ccgtctcgcg cgattatcct
    15901 gtaatgtttg gtaccttgta tattttcacc ctgattggcc tgctgctgaa tattgtcagt
    15961 gatatcagct atacgctggt tgatccgcgt attgattttg agggacgtta atgtcgcgac
    16021 tcagccccgt caatcaggcc cgttgggcgc gttttcgtca taaccgtcgc ggctactggt
    16081 cgttatggat tttcctcgtc ttgtttggtt tgagtttgtg ttcagagctt atcgccaacg
    16141 ataaaccatt gctggtacgt tatgacggca gttggtattt cccgttgttg aaaaactaca
    16201 gcgaaagcga ttttggcggc ccgctggcaa gtcaggctga ttatcaggac ccgtggctga
    16261 aacaacggct ggaaaataac ggctgggtac tgtgggcacc gattcgcttt ggtgctacca
    16321 gtatcaactt tgctaccgat aagcccttcc cttctccacc ctcccggcaa aactggctgg
    16381 gaacggatgc caacggcggc gatgtgctgg cacgcattct ctatggcacg cggatctcgg
    16441 ttctgtttgg cctgatgctg actctctgtt ccagcgtgat gggcgtgctg gcgggggcgc
    16501 tacaaggcta ttacggcggt aaagtcgatc tctggggaca acgctttatt gaagtatggt
    16561 cggggatgcc gacgctgttt ttgattattt tactttccag cgtcgtacag cctaacttct
    16621 ggtggctgct ggcaattact gtcttgtttg gctggatgag tctggtcggc gtggtgcggg
    16681 cggagttttt acgtactcgt aatttcgact acattcgtgc ggcgcaggcg cttggcgtca
    16741 gcgatcgcag tatcatcctg cgtcatatgt tgcctaatgc catggtcgcg accctcacct
    16801 ttttaccgtt tattttatgt agttcgatca ccaccctgac ctcactcgat ttcctcggct
    16861 tcggtctgcc gctcggttca ccgtcactcg gtgaactgct attacaaggg aaaaataacc
    16921 ttcaggctcc gtggcttggg atcaccgcct tcttgtcggt ggcgatatta ttgtctttgc
    16981 tgatctttat tggtgaagcc gtccgcgacg catttgatcc taataaggcg gtgtagcatg
    17041 acgcaaactc tgttagcgat tgaagatttg tcggtgggtt ttcgccatca gcaaaccgta
    17101 cgtacagtag tcaatgatgt ttcactacag attgaggctg gcgaaacgct ggcgctggtg
    17161 ggtgagtcag gttcaggcaa aagcgttacc gcgctgtcaa ttttacgcct gctcccttcc
    17221 ccgccggttg aatatctctc cggcgatatt cgttttcgtg gcgaatcgct gcttcatgcc
    17281 agcgatcaaa cgttgcgcgg tgtacgcggt aataagatcg ccatgatttt tcaagagccg
    17341 atggtgtcgt taaatccatt gcataccctg gaaaaacagc tttatgaagt gctttcactc
    17401 caccgcggga tgcgtcggga agcggctcga ggcgaaattc ttaactgcct tgatcgcgtt
    17461 ggtatccgcc aggcggcaaa acggctgaca gattatccgc atcagctctc cggcggcgaa
    17521 cgccagcggg tgatgattgc gatggcgctg ttaacgcgac cggaattatt aattgccgat
    17581 gaaccgacca ccgcactgga cgtctctgtc caggcgcaga ttttacagct gttgcgcgaa
    17641 ctgcaaggcg agctgaatat gggcatgctg tttattactc ataacctcag cattgtcaga
    17701 aaactggccc accgcgtggc ggtaatgcaa aacggtcgct gtgtcgagca aaattacgcc
    17761 gctacgctat ttgcctcacc cactcatcct tacacacaaa agctactcaa cagtgaaccg
    17821 tcaggcgatc cagtgccgtt gccagaacct gcctcaacgt tgctggatgt tgaacagctt
    17881 caggttgcct tccccattcg caaagggatt ttgaagcgca ttgtggatca taatgtggtg
    17941 gtgaaaaaca tcagttttac gctacgagcg ggtgaaacac tgggtttagt gggcgagtcc
    18001 ggttccggga aaagtacgac gggactggcg ctgctgcgac tgattaattc tcagggcagc
    18061 atcatctttg acggtcagcc actgcaaaat ttaaatcgcc gccagctgtt acctattcgt
    18121 catcgcattc aggtggtatt tcaggatcca aactcctcgc tcaacccacg actcaacgtt
    18181 ttgcagatta ttgaggaagg cttacgggtt caccagccga cgctttctgc cgcacaacgc
    18241 gaacaacaag tgatagccgt gatgcatgaa gtgggattag atcctgaaac acgccaccgt
    18301 tatccggcgg agttctctgg tggtcagcga caacgtattg caattgccag ggcattaatt
    18361 cttaagccct cgctgatcat acttgatgaa ccgacatcat cactcgacaa aacggtacag
    18421 gcgcaaatat tgacgctatt gaaatcattg caacaaaagc tgcaactggc ctatttgttt
    18481 atcagccacg atttgcacgt tgtccgcgcg ttatgtcatc aggttatcgt actgcgacaa
    18541 ggggaagtag tggaacaagg accgtgcgcg cgcgtgtttg ccgcaccgca gcaggagtat
    18601 acgcgtcagc tactggcgtt gagctgacgc ttaaaaagga ttgtagtttg aaaagggttc
    18661 ggcaattgcc acaccaaaat ttttcaatcg gcatgttgca gcaaactcgt cctggcgatt
    18721 tacaaacagg cacggctcac cttcacactc cagcaccgaa cacggaactt cgatgtcgct
    18781 taacgcctgg cttagtttgt acatcaccga ccatgcatta tcaccatccg ggctaagcaa
    18841 tttaagcgcc accaggctgt tgtcaccgca cggtccattt tgcggaatcg gttcaacctt
    18901 cgaacctgcg aaaccagcag accagcgaaa gttctgcggt agacgatgga cgattgagag
    18961 ttgtaatgaa gtcacttatt ttccccggaa gcacatttac ttcacaattt gtttacatca
    19021 attttaacac atcatcaaca atccgtcttt atacagatcg taaagattga tgcctcgcct
    19081 gcgtggcctc tatggctctg atttaagtaa tatctgcggg cgtggtcacg tttgcggggc
    19141 tatatgtacc cgtttctgcg atctaactca acctttttaa ctacaatgat gtgacttttt
    19201 acataaattg attttacata aaataaacat atatcggggg aatgatagat ttgtggttga
    19261 catgcatcaa caaaggaagc cttttagctt cctcgttgtg caatagatca ccgttttttc
    19321 ggtcgactgg cgtacagaca gaagagaatg gagctgcttg cgcagaatgc aattgaccaa
    19381 atcatcggcc atgcagagtt aaaggtcgca agagaaagca atgcgccaac aattgcccct
    19441 atgccaaaac ggaaggttcc tgccagcgaa gatgccgttc ccgccatatg gggaaactca
    19501 tcaagaatga ccgccatcgc attggatgac accatcgaca cacagcccac aaacgccgca
    19561 acgccaacca ccagaatccc agccccagca gcgcactgat gaccatccac gctgccataa
    19621 taaattgtat ccacaacccc gagcggaaca tatttaacgc gccaatgcgg cgaacgaagc
    19681 ggctgttaaa gatggtcatc acgaacagga aaacaatgtt tagtgcaaag taataaccaa
    19741 agttttccgg cgcgacgtgg ttaatttcaa tgtagacaaa cggtccggcg ctcaggaacg
    19801 agaacattcc ggcaaagctg aaaccgctgg ccagcatata actcaggaca cgcttatgac
    19861 ggaacagcgc cgcaaagtta ccaatagtag tacgaatgtg aaaaggctgg cgacgctccg
    19921 gtggtaaggt ttctttgatc agaaagaaga tcattgccga agccagaatc gccgccacgg
    19981 cgaggatcca gaagatgtaa tgccagctca gccacaccag cacccagccg ccaactatcg
    20041 gtgccatcag cggtgcaatg gtcgtcacca gcatgacaaa cgacatcatc cgcgagaact
    20101 cttctttcgg gtagatatca cgcatcaaag cgttgataac catgctggcc gcagccgcag
    20161 ccagcccgtg gaaaaaacgc atcacaatca gctgatcgat ggtttgtgcc aacgcacacg
    20221 ccaccgcggc ggcggcaaac accagcgtac cgccgagcac caccggctta cgcccgaagc
    20281 tgtctgccat cggcccgtag attaactgcc ccaacgcaaa gcccagaata taagtactga
    20341 gggtcatctg cgtactgccc gccggtacgc caaactgcgc tgaaattacc ggtagcgcgg
    20401 gcagatacat atcaatcgac agcggcatca acatggccag caggccaagg ataaaaacaa
    20461 tagcaaacga cgaatgctgt cgggtggtca caacgggctc ctgaaagtca ttgaaaagtt
    20521 agacgacgct ggcaatttct tcttcagtta acggacgata ttcaccgggg gctaaatcag
    20581 catccagcgt aataccgcca atacgttcac gatgcagctc aaccacgtgg ttacccacgg
    20641 cggcgaacat gcgtttcacc tgatgataac gcccttcgct gatggtcaga cgaacctgcg
    20701 ttggggtaat cacttccagc accgcaggct tagtgagatc tttttcgtta tgcagctgca
    20761 cgcctttagc aaattgctct gccgtatcgt cagctacagg tgattccagt gtcaccagat
    20821 aggtcttctc gcaatgatgg cgcggagaag taatgcggtg cgaccactga ccatcatcag
    20881 tcatcagcac cagaccggtg gtatcgatat ccagacgtcc tgcggcgtgc agtttccagg
    20941 ctaccggttc atccagaaaa tagagcaccg ttgggtgatc agggtcgtcc gtggagcaaa
    21001 catagccctg aggcttattg agcatgaagt aacgtggacc atgttgctgc gccagcgggt
    21061 tgccatcgta agcgacatca tgttcaggaa gcagtttgaa cgctgcatta cgaacaattt
    21121 cgccatcgac ggtgacacga ttgccgcgga tttcacgccc ggcaatagca cggctaacgc
    21181 cgagttgctg tgcgataaat ttatcaagtc gcatgtgtgt gattttgcct gtaaaaacgg
    21241 aggtcgggca ttgacccgaa aatctgaact gttgtctgcc cagtatagcg gtctaataac
    21301 atccctcaag ggaaaaagat ccatggcata ctattagcag aataatctac ctacgcgaga
    21361 ccatgatttt tacactccgc ccatatcagc aagaagccgt ggatgccacg ctcaaccatt
    21421 ttcgtcgtca taaaacccct gccgttatcg tgctgcccac cggcgcaggt aaaagcctgg
    21481 tgattgccga actggcacgg ctggcgcgtg gtcgtgttct ggtgctggca cacgtcaaag
    21541 aactggtggc gcaaaaccat gcaaagtatc aggcgctggg gctggaagcc gatatttttg
    21601 ccgctgggtt aaagcgcaaa gagagccacg gtaaagtggt atttggcagc gtgcagtcgg
    21661 tcgctcgtaa tcttgatgcc tttcagggtg aattttcgct gttgattgtc gatgaatgcc
    21721 accgtattgg tgacgatgaa gagagccagt atcagcaaat cctcactcac ctgactaaag
    21781 tgaatcccca cttacgcctg ctggggctga ctgccacgcc ttttcgattg ggcaaaggct
    21841 ggatctacca gttccattat cacggcatgg tacgcggcga tgagaaagcc cttttccgtg
    21901 actgcattta tgagctgccg ctgcgttata tgattaaaca cggctatctg acgccgccag
    21961 aacgactgga tatgccagta gtgcaatacg atttcagccg cttgcaggca cagagtaacg
    22021 ggctgttcag cgaagctgaa ctcaaccgtg agctgaaaaa acagcaacgc attacgccac
    22081 atatcatcag ccagatcatg gagttcgccg aaaagcgcaa aggggtaatg attttcgccg
    22141 ccaccgttga acacgcaaaa gagattgtgg gattactgcc tgccgaagat gcagcactga
    22201 ttactggcga cacccccggc gctgagcgcg atgtgttaat tgaagatttt aaagcccagc
    22261 gttttcgcta tctggtcaac gtcgcggtac tgaccaccgg atttgacgcc ccgcacgtcg
    22321 atcttatcgc cattctgcgt cctaccgaat cagtgagtct ttaccaacaa attgtcgggc
    22381 gcggtctgcg tctcgctcct ggcaagactg attgcttaat tcttgattat gcgggtaatc
    22441 ctcacgacct ctacgcgccg gaagttggta caccgaaagg caaaagtgac aacgtcccgg
    22501 tacaggtttt ctgccctgcc tgcggttttg ccaacacctt ttgggggaaa acgaccgccg
    22561 acgggacatt gattgaacac tttggtcgtc gctgtcaggg atggtttgaa gatgacgacg
    22621 gccatcgcga acagtgtgac ttccgtttcc gttttaaaaa ttgcccgcaa tgtaacgcgg
    22681 aaaacgatat tgccgcccgc cgctgccgcg aatgtgacac ggtactggtt gatccggatg
    22741 atatgttaaa agcggcgcta cgactgaaag acgcgctggt attacgctgt agcggcatgt
    22801 ctttacagca tgggcacgac gagaaaggcg aatggttgaa aatcacctat tacgatgaag
    22861 acggcgcgga tgtgagtgag cgtttccgtc tgcaaacgcc cgcccagcga actgccttcg
    22921 agcagctttt tatccgcccg catacgcgca caccgggcat ccctctgcgc tggatcaccg
    22981 ccgccgatat cctcgcccag caagccttac tgcgacatcc ggattttgtc gtcgcccgca
    23041 tgaaaggtca gtactggcag gtgcgtgaaa aagtgttcga ttacgaaggt cgttttcgtc
    23101 gggcgcacga attacgcggt taatggtagt tttgattgat gtataaggcg attgagtata
    23161 gaatctcgcc cgcttttgca tacgcaaagc agatcactta cctgttgctg ggtcgcctgt
    23221 agcaggatta atttaagaga gaaagaaatg tttactatca acgcagaagt acgtaaagag
    23281 cagggtaagg gtgcgagccg ccgcctgcgt gccgctaaca agttcccggc aatcatctac
    23341 ggtggcaaag aagcaccgct ggctatcgag ctggatcacg acaaagtcat gaacatgcaa
    23401 gctaaagctg aattctacag cgaagttctg gccatcgttg ttgacggtaa agaaatcaaa
    23461 gttaaagctc aggacgtaca gcgtcacccg tacaaaccga agctgcagca catcgacttc
    23521 gttcgcgctt aattgctgaa taagttgtaa aaaaccccgc tccggcgggg ttttttgtat
    23581 ctgcagatta tgcctgatta cggtattgct attttttgca ggccagataa ggcgttcacg
    23641 ccgcatctgg cattcagaat acaaaaccga ttaattgccg ccagacgtcc ggcgctgcaa
    23701 ttggtcgcgc aaattcggcg gcgtgccttt aatggtcaag gtatcggttg ctggatccca
    23761 gaagatgcgt tcacctaaca gcatcgcatc aaagttaata gtcaagccac cgccgctacc
    23821 ggcaaacttc gtcaactgac gcagtgtgct acggtctgcc gggaagctct cttccagttc
    23881 gtagcctttt tcagccgcaa acttcgtgaa gctcacttcg cttacgcctg ccagctcttt
    23941 cgacagcgat tccagttcga tctcttcgcc tgcctgcagc tgctcattac agtagctata
    24001 aacctgctgg cgcacgttct gtcgctctgc tttatccagt tgtgcttcgg cagtgaaatc
    24061 atcaaccgct tgcaacagcc cacggttttg cgctttcgcg ttcaatcctt cgctggcccc
    24121 gaggaaatcc ataaagaaat ccgccacttt gcgccctact cgccctttca ggaaagtgag
    24181 atagcgggtg gactctggat tggtttccca ttcggttaaa tcgatacgcg caacaatatc
    24241 cgcatgattg atatcaagat aatgggttgg gttgatgtcg agattttcgt taacgcgcat
    24301 actgctcagg ttgctcagaa ctgccaccag cagatactcc accgccagat aacggtagtg
    24361 gcaaaacagc acgaatccac cgtcagcaaa aggatatttc gctaattcgt cacgcaggcg
    24421 cccggttgcc gcgcggctaa atgccagaaa gtcctcttcg ccctgacgct gtaagcgcag
    24481 cgtctgcgcc agttcactct cttcgctaaa caagccgtaa gctttatttt tggcgctata
    24541 gacccgatgc agttctgcca ccatctcaac gacggtttct gtcggttcca gcaatgaatc
    24601 gcgcaacacc agctcaaggt tctgctcatc acgcttgata agctggtgca gagcaatctg
    24661 gttgatatcc agactcatga taaactctcc tttaagaccg ggcggtattc aaccaccgcc
    24721 ggttaagcga cgcaataaaa gatggcggtc attttacaga aggcagcgta acgagaatga
    24781 tttaacgcat aaaaaagcag aaaaaaaacc gttgctacgg taatatgttg ccctttcatc
    24841 aactaactga tttcgattta tgccacaaat ttcccgctac agtgatgaac aggttgaaca
    24901 attgctcgca gagctgctca acgtactgga aaaacataag gctccgaccg acctttccct
    24961 gatggtgtta ggcaacatgg tcactaacct tatcaacacc agcattgccc cggcccaacg
    25021 ccaggcaatt gccaactctt ttgcccgcgc cttacagtcc tctatcaacg aagacaaagc
    25081 gcactaaggg aaacagataa caggttatgg taactcatcg tcagcgctac cgtgaaaaag
    25141 tctcccagat ggtcagttgg gggcactggt ttgcactgtt caatattctg ctttcgctcg
    25201 tcattggcag ccgttacctg tttatcgccg actggccgac aacgcttgct ggtcgcattt
    25261 attcctacgt aagcattatc ggccatttca gcttcctggt gttcgccacc tacttgctga
    25321 tcctcttccc gctgaccttt atcgtcggct cccagaggct gatgaggttt ttgtccgtca
    25381 ttctggcaac ggcgggaatg acgctattac tgatcgatag cgaagtcttt actcgtttcc
    25441 atctccatct taatcccatc gtctggcagt tggttatcaa cccagacgaa aatgagatgg
    25501 cgcgcgactg gcagctgatg ttcatcagcg tgccggttat tttattgctt gaactggtgt
    25561 ttgcgacgtg gagctggcaa aagctgcgca gcctgacgcg tcgtcgacgc ttcgcgcgcc
    25621 cgctggccgc attcttattt atcgccttta tcgcctcgca tgtggtgtat atctgggccg
    25681 atgccaactt ctatcgcccg atcaccatgc agcgcgctaa cctgccgctt tcgtacccga
    25741 tgacggcgcg acgtttcctt gagaagcatg gcctgcttga tgcgcaggag tatcaacgcc
    25801 gtcttattga gcaaggtaat ccagacgccg tttccgtaca gtatccgtta agcgaactgc
    25861 gctatcgcga tatgggcacc gggcagaatg tgctgttgat tactgtcgat ggcctgaact
    25921 actcacgctt cgagaagcag atgcctgcgc tggcaggttt tgctgagcaa aatatttcgt
    25981 tcacgcgcca tatgagctcc ggcaacacta cagacaacgg catctttggc ctgttctatg
    26041 gcatctcgcc gagctatatg gacggcattc tgtcgacccg tacccctgcg gcgttaatta
    26101 ctgcgcttaa tcagcaaggc tatcagctgg gattattctc gtcagatggc tttaccagtc
    26161 cgctgtatcg ccaggcattg ttgtcagatt tctcgatgcc gagcgtacgc ccccaatccg
    26221 acgagcagac cgccacgcag tggatcaact ggctgggccg ctacgcacaa gaagataacc
    26281 gctggttctc gtgggtctct ttcaatggca ctaacattga cgacagcaat cagcaggcat
    26341 ttgcacggaa atatagccgg gcggcaggca atgtcgatga ccagatcaac cgcgtgctca
    26401 atgcactgct tgattctggc aaactggaca atacggttgt gattatcact gccggtcggg
    26461 gtattccgct gagcgaagac gaagaaacct ttgactggtc ccacggtcat ctgcaggtgc
    26521 cattagtgat tcactggcca ggcacgccgg cgcagcgtat taatgcgctg actgatcata
    26581 ccgatctgat gacgacgctg atgcaacgcc tgctacatgt cagcacacct gccagcgaat
    26641 attcgcaagg tcaggatttg ttcaaccctc aacgccgtca ttactgggtc actgcagcgg
    26701 ataacgatac gctggcaatt accaccccga aaaagacgct ggtgctgaac aataacggta
    26761 aataccgcac ttacaactta cgtggtgaaa gagtgaaaga tgaaaaacca cagttaagtt
    26821 tgttattgca agtgctgaca gacgagaagc gttttatcgc taactgatta attataaatc
    26881 agttagcgaa atatcttact tgcaatcggt gtggaaaacg gtagtattag cagccacgag
    26941 tcggcacgta gcgcagcctg gtagcgcacc gtcatggggt gtcgggggtc ggaggttcaa
    27001 atcctctcgt gccgaccaaa tcgcccaccg gataccaccg gaattaacag tttttagaca
    27061 cgaaaagccc gctaatcctt gattagcggg cttttttatg gtttttagtc accggacatc
    27121 accggattta taagctatca gccggatttt ttagttatac gcttagttat acgggcttgc
    27181 gtataactaa agccgtataa ctaaaaccct gttttttcgt gtggaatcat atacatatgg
    27241 cgagaaaaac acccccatta accacggtgc agatcaaagc agccagacca gcggaaaagg
    27301 aatacaccct acaggacggc ggagggcttt ttctcctggt caaaccgtcc ggatcaaaac
    27361 tctggcgatt ttcctactac cggccatcgg acaaaaaaag aatattgctg agttttggat
    27421 cgcttgatga tgtttccctg gctgatgcca gaaaacgccg tagcgagtac agagcgttaa
    27481 tcagtgccgg aactgacccg cagggccatg agaagaaaaa acgagaggca gaggcccgaa
    27541 gacaagggaa cacgttcgaa aatgtggcgg cggcatggta ccaggtgaaa atcagccaga
    27601 atctggcccc caacacgatt aaagacatct ggcgttcgct ggataaatat gtattcccgt
    27661 tcatcggcaa cacgccaata gataccctca ccgcccgaag gttcgttgaa gtgcttacac
    27721 ccatcaagga gcgcggcaac ctggaaacac tcaagcgggt tttacagcgc gttaatgagg
    27781 taatggatta cgccgccaac agtgggctga ttgatgccaa tccggctatg aatgtgcgta
    27841 aggcgtttcc ctcacctgta aaaaaacata tgccaacaat ccgccccgaa cagctgccgg
    27901 agcttatgca ggctttatca gtatcggcaa cagaacggca gaccagatta ctgattgaat
    27961 ggcagttact gaccgtaacc cgtcccgccg aagcgtcatc aacgcggtgg gatgaaatca
    28021 acctggacgc gaagcaatgg acgatacctg ccggacgcat gaagatgcgc agggatcacg
    28081 ttatcccgct ttccggtcag gctatggcgg tgctggaggc catgaaacca atcagccacc
    28141 accgcaatta cgttttccca agtctgaaag acccacagca gccgatgaac agccagacag
    28201 ctaacgcagc attgcggcgt atgggattcg ctggcgtgct ggtgtctcat ggattacgcg
    28261 ccatattcag cacagcagcg aacgaggaag gattcgagcc ggacgtaata gaggcggcac
    28321 ttgcccacgt cgacaccaac gaagttagac gggcatacaa ccggagcaac tacatagaaa
    28381 aacgcatcgt gctgatgcgc tggtggggcg aatttgtcga ggctgcggag acgggcgtaa
    28441 ccctcgccag tggtaaaagg ggtatccgag ccgtgtagct gtacagaaaa ccagtaaaaa
    28501 ctacgaaaac catgtaaaac cgtcgtataa ttgcatcaaa tttaacgaca aggccgtgaa
    28561 acatgaaacc gttaagatgc aaaaaaatat cagatgcaat tgcgacgggc tgcaactggc
    28621 cctgatggtt cagcatgaat tttggtcaac ctacgatccg gaggacagaa cgacggcccc
    28681 atcaaaaaaa caggtagtag attttctggt atcccgtggc gcttccagaa atctggcggt
    28741 aagtattgat aaggtcgtac gtccggcatc tatgaagatc ggaggcaggc ccaaaaaatg
    28801 gcggtaacaa tcctggaagc ggcagaaatg ccgctttttt tataattcca tttcaaatca
    28861 tcaatataaa aacggtgtat accgtttaaa aacggtggga actgttttta cccatatccg
    28921 atgatttacc gtatttgtca ccggaataca ccggattcac aaggtaaatc acgatggaag
    28981 caatcagaaa aattatcttt cgccaggaag taaaaaaaat tatccacatc aaggcagaca
    29041 gcacgctgca aagcatgatc aacgccggag aatttccgca gggttttcgc gttggtttac
    29101 gccgtcgcgg atggtatgag gatgatgtgt tggcctggca gaaagaacgc gaacaggaag
    29161 cacgcggaac ggctgcttaa cgggtatcac agatatgaca aacacgaaaa aagcggcccc
    29221 gatatggagc cgcctttctg aacaattaac ccgctgcgcc ttatgtgtat atgatcccaa
    29281 acataagcac ggggatgata gccgctatca ggctggtggg caatgcaatc agtctggttc
    29341 agttcgttgc catacctgca atgagcgctt ttccctatac tctttaagga attgctcaag
    29401 ggcaaaagca catggtgcga atctttctga ttcatgctct atctttctgc gccgtctttt
    29461 ccgtgccggt gataatgttt tggtcaattc tttatcggtc attgtgttgt cctgcatagc
    29521 aatgcgccgt aatacctcac accacggcgc tgatagtttt tatcctttgg gttctatgcc
    29581 gcgccgcttt agttcagtgc gccccaactc tttaagccag ttggctaggc ttatgccgtc
    29641 gctctgtgct tctttgtcga attgctcttt tagctctgga gaaattcgca ttctgaattg
    29701 tggggattgc ccgtcccctt tagggctttt atcgcgtttg attattgaca agtggtcacc
    29761 tattgaatta gcctttccat tgttaggtgg ccaccttaac acaagagcac ttaaaaaagc
    29821 aaagccccgc aagtgtcatt accactcgca gggcttctaa ccaacaacgt aaactaggag
    29881 ccgttatggt tgccgtaaat catataccac accttgtaca cacacaaacg gcctttgtgt
    29941 ggcgttttct ggccctgagt gccggagaat ctcaaatcat ccacgtaacc gcctggacgg
    30001 aacgcgaagc gcgtagccgt tgcccgtccg gttgtgttgc tgtattcgcc gcccgtattc
    30061 gtcaggggga aacctatgca caataaaacc acaccgaacg cagcagccgc cgcactcact
    30121 acgctgatgc acgcgcttat tgatattgaa tgcactgcag agcttgcgca gggagaagaa
    30181 cagaaagacc ggacacagtt cgccctggaa tgtatccgat acatcgcaac gcggtcgctg
    30241 aatgacgcta aaaatattct tgttgctgat tgtgaaaatg gggggggtta tgcgtgatga
    30301 tcgttttaat tccctgaaac aggaatttcc cggcgttcct gatgatgcgg ctgatgcgct
    30361 ttcgtcaatg ccagaactta ttagagcggc ttttttctta ctttccacga gagaatataa
    30421 atcaacgggg cttgatgtac tgaatatcgc cgccgattat gcggaatatg tggcagaggc
    30481 gcgttacaga agaaaatttc ctgaggatgt aagccatgcg tgatatttac cacgaaacaa
    30541 tagaccgcgc atttcttgca ctttctcaca gtgaaaacat gctggaaata ttgcgcatat
    30601 ggcttgaaac acttggcgac aatgaacgcg acaaacaaaa atcaagaatt gccacggcat
    30661 taataacgct tcttgagcct gtaataatgg aactgcaaga aatagatcta ttgcacgaca
    30721 gatataaaga acagcacacc ggagaataaa aataatgaaa cttaaatatt ctggcttaac
    30781 tgccagtggc aacactcacc ctaaatttac gcgcggtgat atttaccgcg accagtacgg
    30841 cggcacggta atgattaagg gcgtggaaga acggcgtgta acctaccgcc gtgaaggtta
    30901 cgaatatgat tgcgtgatgc ctgtttatca gttccggcgt gatttttctc tggtacagac
    30961 cgcgccgcat aacgtgccca ccagcaacgc cagggcacgg gcaaacatcc agaagctgaa
    31021 aaccatgatt aacggattca ggggcaagaa atgaaaagcg caccgaactt aaaaaaacag
    31081 ccttacgaca agatgaccga agtcattatt tttgcgggta gtgatgcctg ggcacatgca
    31141 aaacagtggc aggaacagga cgggcgactg gctggcgata atgtgcctcc cgttgtgctg
    31201 gctgatgatc aactggatga actggcagac ctgagaatca tcgacgaggg gcgctattgt
    31261 gtccggctgt acaaggcagg ccacatcagg ccatcaaata ttaatgccat tgcgcacaag
    31321 ctggcggcgg cgggtgtaac tgatgcgaat tattaccccg aagggatgca cagccatatg
    31381 cgggagaact ggcgcgaata cctggaacgg gtgcgcggga aagagccggt ggaagaaaaa
    31441 aaccaccagc gaaaaaccac gctaccgatg agcgttggat ctaccggata cgacacgcaa
    31501 ctggattacg tggttaaggg gattattccg gcggtatcgc tatgcagcat atacggggct
    31561 agcgggtcct ataaatcatt ccttgccgga tcgtgggcgt gccatgttgc cactggtcgc
    31621 cagtggggag gccgcagggt tgcacatggt gcggttctct atgtggttgg tgaaggcggt
    31681 atcggcgttc cgcgtcgtgt aaaagcctgg gaggttgtgc acgatgagca ggtgaaaaat
    31741 ctgtatctgg taaaccgccc catctttccg gctgccccgc ttgatgttga tgaaatggtt
    31801 atcgctgccc gtcaggtgga gcgggaaacg ggtaaacctg tacgcatgat tattctggat
    31861 acgctggcgc gttgctttgg tgggaatgat gaaaatgatt cccgtgatat gggggcgttt
    31921 atccgtggtt gtgacgaact gaaacgacgc acaggggcca cggtgctggt ggttcaccat
    31981 tccggcaagg atgagacgaa aggcgcgcgc ggttccagtg catttcgtgc ttcgctggat
    32041 gctgaatacc ggatacgcag ggaggacgca ggaagcaaag cgctggttat ctcatgcacc
    32101 aaaatgaagg acgcggagga actcaaagaa gccgcatatg acttacgcgt ggtggagctt
    32161 tttaccgacg ctgacggtga attaatcacg tcgctggtgg tggtggatga tccgcgccct
    32221 cctgttgaac tggagcgcat cgaggaggca gggaacaaga cggaaaacca taccgcgcta
    32281 tgggggtgca tccgttcacg cacacagaac ggcgacaagt gcacgatccc gctgttacgt
    32341 gatgacatga aaaagctggg gtatgaaatg aaaaacttcc ggcgctggct gtacaagctg
    32401 gaaaaagatg gggttattag tatcgatggg gatgatgtag cgccgctata aaagtgagga
    32461 gcaaaagcga gggggataga aagagggcca aaattagccc gctctccctc acttttcgac
    32521 ctgtatacat cctcaaaagt gaggggtaaa aaaaatactt ataaaacaca cacatagaaa
    32581 aaccgaaaat cccaactgcg acgaagtgag acgcttgaaa aagtgaggcg aaaaagtgag
    32641 aggttgcgag aaatgaccca aaaacgcaga gacagaacag agccaaaata taaagcgtta
    32701 gacatgactg agcacacctt aaaggtggca atcagaacga tagaccgcca cacgcgggaa
    32761 ggatacgcga aggaacatcc cgacctgata agcgcattca tgaccacggc ggcggcaaac
    32821 tttgccacgc tgacagaacg ggagattgcc gaagcggaac aggtaacaac catcaacgtt
    32881 aaaaccggag aggtggaatt atgacggcac agatagccgc ttacgggcgg ctggtggacg
    32941 acccgcaggt aaaacagacc agcaagggca caccgatgac gctggcgcgt atggcggtat
    33001 ctttgccatg cagccaggca caggacggac aggcgacgtt atggttatcg gtcatcgcat
    33061 ttggcaaaca ggccgacttc ctggctaaac atcaaaaagg cgatgttgcc agcgtatccg
    33121 gcacgatgca ggtaagccag tggaccggac agaacggaga aacgcggcag ggctggcagg
    33181 ttatcgcaga cagcgtaatc agtgcccgcg cggcacgtcc aggcgggaac agacgcaaaa
    33241 ccacaggcac acagggtaat cagccaccag cgggaggcga tgacccttac ggtgatgata
    33301 tttcgttctg agggggtgac gatggtacat gaccgcatag cggaggaact ggaggcgaaa
    33361 ggcttttacc ggagggcggc ggcgcgatgg ggtgaagtca tgcagctggt ggagacagac
    33421 aaggaacggc atcacatcac gatgcgacgg ctggaatgtt caaggaaggc acagagggca
    33481 ccggagccgc cggataattt cggagacctg aaaaaggcag tcgatcgcac ttatgccgaa
    33541 atgggtatag atggtgctgg tgatgaaata tggcgcaatt accaggacag ctaatcaaac
    33601 agccggagaa atccggcttt tttgcaccag ttgaaacggt atggcgcatt accgggtttt
    33661 cgtcacggtc aggcatagtt actatctgaa acaaacagac acaacagagg aaaaaaacaa
    33721 tgccgatgaa atttgatgag atattaaaac agcgtgataa ataccatgct gacaacatgg
    33781 agacgatgag catcaatgat taccgcgcat tcctggagac gggcgcactg attgaaaagg
    33841 atcagcatgg ttttgtgaga tgtgctctat ccggtgaaat gctggcggta aatcctgaac
    33901 agatagatgc attgatagaa tttctgaaag agatcagaga ctgatccagc acacagcaca
    33961 catagccgga gcaatccggc ttttttgcgc ccaaaaaaag cccgataagg tcagagggtt
    34021 cttatcgggc ttttgcatat gaggtttttg gatgcactgg cgttcgtgat cgggataatc
    34081 atttcataat ttgcaacata actcaatatt attgcataaa atgcaattct gattataatc
    34141 aggactggat aaacatccag ttatgatttt tttagtcgaa gaggaatttc ttactatggc
    34201 tgaagagaaa aaaggcggtg tttcggtgta cataagcccc gaaatcgtgg aggtgctcaa
    34261 gcagcgccac aaaaaaaact atgaggctgg cgtggcggct ggactggatc cgctgatgac
    34321 gccggagccg tcgataggtt cacttgtacg ctcttattta cttgcggcgc ttgggatgca
    34381 taaaaattat gggggtgaat aatggcaggc aaagcaacgg cacttaacac taaccagctt
    34441 tttatgtacc tgaatcgcgg ggatattgcg gattttaaat tcagccctct gtttaccacg
    34501 ctgtttttcc cgaacgtggc gacattcagc acgcaaaaca tcatgctgga taccctggac
    34561 attgaagaag tcaccatgtc ggcgttttgt tcgcctatgg tgggtagcca ggttcagcgc
    34621 gataaagggt acgaaaccag cacaatcaaa cctggctaca tgaagccaaa gcacgaaatc
    34681 gatccaacga aaaccatcat gcgcatggct ggagaagatc cggcacagct taacgaccct
    34741 acctatcgcc gtatgcgcct gattactggc aacatgcgcc gccagataaa cgccattaag
    34801 gcacgcgtgg aatggctggc ggtgaatgcg gtaacgaccg gaaaaaacat cattgagggc
    34861 gaaggcatag aacgctatga aatcgactgg aagataccgg aaaaaaacat catagagcag
    34921 tccgacggca aaaaatggtc cgagcatgat aaagatattt acgatccaat ctatgacatc
    34981 gaactatacg cagatcaggc aggttgcccc gccaacgtca tgattatggg cgctgaggta
    35041 tggcgcacgt tacgcagctt taaaaaattc cgtgaactgt acgatctttc ccgtggttca
    35101 gaatccgccg cagaaatggc ctgtaaaaac ctgggcgaag tggtgagctt taaaggctat
    35161 ctgggcgata ttgctcttat tgtctattcc ggcaaataca ctgacagcga cggcaccgaa
    35221 aaatatttcc ttgagcctga tttgctggtc ctgggcaaca ccaacaataa agggctggtg
    35281 gcctatggtg cgattatgga tcaggaagcg gtaagaacgg gcgcaacgca aaacatgtac
    35341 tacccgaaaa actggattga ggacggcgat ccggcgattg agtacgtgca gacacacagc
    35401 gcaccgcagc cggttccggc agatattcgc aaatttgtta ccgtcaaaat tggttaacgg
    35461 gggattatat ggacactcca tatattgagt tatttgcagg cagtcagcag gtatcaacga
    35521 cactggtaca ttttgccgct gatgctggcg ttattcagga atttaccccg ctgatgctgg
    35581 tggacaatgg cgagtttaag ccgtgggatg gtcaggaatc tggcaaggct gtttatctga
    35641 cttcgtacct cgtggacacg tcaaagcaga aatcagcaca gtgttacaag acggggatat
    35701 tcaatattgc cgccgttaac tggcctgaga gcgtcgacac cgatgcgaaa aaatgcgccg
    35761 cctttgcggg ttctggcgta tccgttcagc cgctggcccg ataagcaggg ggaacgatgg
    35821 caacgaatga aagcatcatg gcgctaccgc tggcgagtaa atttaaagcc gaagcgcggg
    35881 caatggctga cagaggttta tcaacctacg aggccgtata tcaactcaac aaactggaag
    35941 agcaggacaa gccgcgcgct gatgcgatta tggcgcttca tgagcataaa gactatcagc
    36001 cgctgttgcg tgcaatggca aacgtgcctt gtatcgatgt tgatacggct aaaaacatcc
    36061 tgaacatgac catagagcag gaacgcccga aggttgcacc agagcttacc gcagcctttg
    36121 aaaactttat ggacatgcac agcccacaaa ccgtatcagc tggcatggca tacgatggca
    36181 gaaacccagg cgatgacggc gacatcgatc gcatactgaa aaccatctga gacaaggccg
    36241 gagaaatccg gcttttttgc gggtccttcc tggaattatg gcccgttacg gggcggcgac
    36301 ctcgcgggtt ttcgctattt atgacgtttt tccgtgaagg tgacaccacc accacttgat
    36361 taatatttaa ccatgcagtt aaggtaacat tatgattgat aaagcttgtt ttgtaagtca
    36421 gcaggaaata gctgaacatt tcaaggttaa cagaaccact attcgcgcat ggaccaaaca
    36481 ggggatgccg tatcttaatg cggatcgcgg aaagtctggc ggttatcaca tcgggcatac
    36541 attgctttgg tcttcaggta aaagccgtct tgaggccatc agatatcacg tagaaaccag
    36601 tgcgctggaa aaaattatgt ttgccaggct gctttcatct gagcgtgacg agtactccag
    36661 tgaagaaaca gaacatcgat ttgatgaagg tttgcagatt tacggctatt caccggaaga
    36721 tgtgagcaag gcacgaaata aaatggctgg ctttctggct gggtggcgtc atgccgtaag
    36781 cgttcgccgt gccagcatgg agcaatcagc cgatacagaa cagtaaagcc gataacttgt
    36841 taatcactcg cagcaatgca tgtgatttgt gcacactttc ggttaactgg ttcgatgtcc
    36901 ggttttagtg tctgtttttt gcgcatgtcc ggttcatgga aagcatgttt ttatattttt
    36961 catatggtta acttgtagag aaaccggaca tggatcccga aaaattttca taaatagtga
    37021 aaacgcgcga ggtcgccgcc ccgtaacggg tccataattc caggaaggac ccgacgacac
    37081 cagacaatca gaacgatagg tgcacaatga cagaagccga aatactggga ttaatccgcc
    37141 gtgctggtgg aatcagccag cagactgacg aacaggccac gcagccggac agcgtgacag
    37201 ccgaaaatta tgcgcgtgtt gttgctgagg tgatgcgccg tgatggtatc cagcttaatg
    37261 atgtggatat gcgcaacata cggatccgcg ttcttgaaat gctggcctac aatcgccgcg
    37321 tggcactgta tcgggaaacg gagaaaatta cataccactg gaagaagccg gagcggttgc
    37381 ggcggtaact ggttgatatt ttcgaaattc gccagcggcg aacatcgggg aaaccagcct
    37441 aacccgttga tattttcggg aacctcaaat tgaggaggtc ggaaacataa cccgtcatat
    37501 tctggatgct gataattacc acgcagccca ttaaggccta acccgttgat tctttcggaa
    37561 acagccattg gcgtaatccg gcgcggtaac ttgctgaact taaagcaaag cgcaaaattg
    37621 cgctggctgg tgggcatgat gccgatatgg tgatccccat aacggaaata tccaccagca
    37681 cggcaacggc agaatagcca caacacagaa aaaccacgaa tatggggttt ttgttatggc
    37741 atgggcatga tgaccgcccc tgctctgaat ggtggaaatc accacgcaga tcatccacca
    37801 gcttcgtttt tataaattaa acatccggga aaatccgacc ctgaattgtg cattttttca
    37861 gcaaatcatt ttagttatac gtttggttat acggtttaaa agttgaatca tatacattat
    37921 tttaaatcat gaagttacac gctcaattaa actactctcg tgaaaaatcc caagaaaaaa
    37981 ccaaccttta cggttggttt ttttatatct gcaattaatt tgataaacag accgtgacac
    38041 atcacagcct gtttattttc tgttatcaga acgtccagac cactcccgcc tgagtattcc
    38101 acggcgattc tacccctgcg ccattgccat agctgactga caaatgaccg cttaacgtcg
    38161 gggtaaacga tgaccttatc ccagcctgat aaacgccagg ggttccagcc acatcattat
    38221 taaatttgcc atcgtcatta actttcacat ggttagaatc ggcgtattcc tgacgtacgg
    38281 cggctttcag ccagggttcc agcgtcgtac cattttgcag gtccatgtga tagcttaccg
    38341 ccgttcccgc ttcagcgcgt aatatccggg tatttcccac atcagcgcgc atgccgtttg
    38401 ataacgtgta gtcctgacca tctgtggtaa agccggtaaa ggccagatag ggtctaacac
    38461 tccacaatcc gtcaacccaa cggaacccgc tctcaacatg agcacccgcg ccgttactat
    38521 tgtaatcgcc aaacgctgtt gccccattac tcatcttgcc atggatggtg ttggcaaaac
    38581 ggtcaacttt caccacccca tcaacatagg caccgttctg atgctcccaa ccggcataag
    38641 cccccagggt atagctatcg atattacctt ttccgccgcg atcaaaacca atatcagaat
    38701 gagagtaacc aaagaacaag ccgcgaattg tactgctttc ttcacgggag aaacggctat
    38761 cgataccgag cgtcaggccc gtcaatgttt gctcaaaacc agctcccgca tcagtggtca
    38821 cgttgttgcg ggtgttaatt gccgaactcc acatcgccgt atcgtaacta acgcttttta
    38881 cgctaccaag acgctcacgc acggtgtcca gttctgcatc aaataccagc ggttgtgcgg
    38941 ccgccatatt cagcacatca gtggttgaag gggtaatttg cgcgcgattc tctgccaaac
    39001 tccagctatg gttgccatta tccagcaagg tatattcata cgtaccgata tcaacaacgc
    39061 ctccggcatt gcccaacgta aatgcagcat caccgccgcc cgttgttacc agtgtaaggc
    39121 tatctcctgc tgccgggctg gcaccggtgt ccgtcacgaa tattttgaaa tcacctgttg
    39181 cctgaccggt gacgttgagc tgatcgctct gatgattagc catatcggta cgcatataaa
    39241 aattgccgtt tcccgacagg gtattggtgg tcaacgtaat gaaattttcc gcacttgccg
    39301 tcactgatga acgagtcact ggctcatcca tcataatcac atttgcgtca gacatcgcaa
    39361 ggttggctaa gttgaggagg gtgttttggt tcgtcgtagt gatatcggca atgatggccc
    39421 atacgctatt ttcgagcgaa acgtccgctt tgctggtatc cacggcacca tgcgttctaa
    39481 aagaagcgcc ctcagaaacc actaccttac ccaccaacat agcgggtgca taatttattt
    39541 gcggcttcat gacctcaaga atgccatcat tccctctgac tgacacgtta accattgtgc
    39601 cagcccagac attagcgcgg ccattgttga taaccatatt ttcagagatg gctttagagg
    39661 aatacgtgat acttccattc tggtctgatc gaccgagatc atataccgca tttgcctgca
    39721 ctttcgtacc agtatccttt cccagcgatt gcatagtggc atgctcatca aggatagtat
    39781 caatggcctg cgtgtcctcc ataacaatga gcccggaacc atcatccagt tcataatttt
    39841 ttgacacacc atcttttata ctaaattggc ctttactgtt tgtaccactc acttccagcg
    39901 cattcgttga gacaattaat tttccgcctg cttttttatc aacgccagtt gcagtcccgc
    39961 catccataac ctccagtaag ccgccactat ctacagtagt attataagcg aagtcatttt
    40021 cttcaactcg caaacttccg ccgttttcca acagcatatt gttagcaata ccattcttga
    40081 tttcgaactg accgagacgg tttattccga atgcctccat gacccgcgtg cttgctttaa
    40141 tagcaccgcc tgctttctga tctaccgcaa tcgctaatcc cccctcttta acctccagta
    40201 atcctccctt ttcgatagcg gcaaggacgg taatcccatc tttttcaacg attaagcgcc
    40261 cgcctttaat cgtgggatca ttcgctgcgc cataaacctg tagcacacca ttattgaggg
    40321 tagtctggtt tgccgcgcca cccatttgaa caatttgata accgtcattt aatactgagt
    40381 attctgcggt accattcatt tcaatgtact gataccctcc gt
//
LOCUS       DADLFA010000013        38853 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000013 DADLFA010000000
VERSION     DADLFA010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38853
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.013"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..790
                     /locus_tag="JQH65_000600"
     CDS             <1..790
                     /locus_tag="JQH65_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY6138106.1"
                     /translation="DSLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQT
                     GVCQGDTSRYGPVNIGANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGD
                     FHDLVKLSIQGNLTAPQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITY
                     DCGDTSKIKNSLQMRIDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQ
                     NGILPVDPSGHGTVNMRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(833..1693)
                     /gene="rsmI"
                     /locus_tag="JQH65_000601"
     CDS             complement(833..1693)
                     /gene="rsmI"
                     /locus_tag="JQH65_000601"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAY6138107.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALVAEIHGVKKNA
                     LYKYALEQQG"
     gene            1757..3784
                     /locus_tag="JQH65_000602"
     CDS             1757..3784
                     /locus_tag="JQH65_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HAY6138108.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKKQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAADVAEQPQPQTADGVASPAQASVSDLTGDQPAAQPVP
                     VSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNVEEL
                     LKSNTPLNVLVLNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIPRSS
                     LGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATTDSG
                     MTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGAMLYAS
                     SRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMGVDA
                     WSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            3742..4137
                     /locus_tag="JQH65_000603"
     CDS             3742..4137
                     /locus_tag="JQH65_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HAY6138109.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGWTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            4157..4747
                     /gene="diaA"
                     /locus_tag="JQH65_000604"
     CDS             4157..4747
                     /gene="diaA"
                     /locus_tag="JQH65_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HAY6138110.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4757..5332
                     /gene="yraP"
                     /locus_tag="JQH65_000605"
     CDS             4757..5332
                     /gene="yraP"
                     /locus_tag="JQH65_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HAY6138111.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(5446..6486)
                     /locus_tag="JQH65_000606"
     CDS             complement(5446..6486)
                     /locus_tag="JQH65_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAY6138112.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQHYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAICLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEARSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(6559..7194)
                     /locus_tag="JQH65_000607"
     CDS             complement(6559..7194)
                     /locus_tag="JQH65_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAY6138113.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            7322..7840
                     /gene="yhbO"
                     /locus_tag="JQH65_000608"
     CDS             7322..7840
                     /gene="yhbO"
                     /locus_tag="JQH65_000608"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HAY6138114.1"
                     /translation="MSKKIAVLISDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7820..8263)
                     /locus_tag="JQH65_000609"
     CDS             complement(7820..8263)
                     /locus_tag="JQH65_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HAY6138115.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     HRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            8314..8616
                     /gene="yhbQ"
                     /locus_tag="JQH65_000610"
     CDS             8314..8616
                     /gene="yhbQ"
                     /locus_tag="JQH65_000610"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HAY6138116.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(8603..9106)
                     /locus_tag="JQH65_000611"
     CDS             complement(8603..9106)
                     /locus_tag="JQH65_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY6138117.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(9100..9624)
                     /locus_tag="JQH65_000612"
     CDS             complement(9100..9624)
                     /locus_tag="JQH65_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HAY6138118.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADVSDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLHVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TTPETKQTSVGEPC"
     gene            9833..10813
                     /locus_tag="JQH65_000613"
     CDS             9833..10813
                     /locus_tag="JQH65_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAY6138119.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPNIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10822..11700
                     /locus_tag="JQH65_000614"
     CDS             10822..11700
                     /locus_tag="JQH65_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAY6138120.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELTSMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLLLTANS
                     DCNGYWRRLAGLELQA"
     gene            11906..12913
                     /locus_tag="JQH65_000615"
     CDS             11906..12913
                     /locus_tag="JQH65_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HAY6138121.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(13031..14275)
                     /gene="mtr"
                     /locus_tag="JQH65_000616"
     CDS             complement(13031..14275)
                     /gene="mtr"
                     /locus_tag="JQH65_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HAY6138122.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAMGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(14429..16318)
                     /locus_tag="JQH65_000617"
     CDS             complement(14429..16318)
                     /locus_tag="JQH65_000617"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH family ATP-dependent RNA helicase"
                     /protein_id="HAY6138123.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(16311..16391)
                     /gene="yrbN"
                     /locus_tag="JQH65_000618"
     CDS             complement(16311..16391)
                     /gene="yrbN"
                     /locus_tag="JQH65_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HAY6138124.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(16498..17382)
                     /gene="nlpI"
                     /locus_tag="JQH65_000619"
     CDS             complement(16498..17382)
                     /gene="nlpI"
                     /locus_tag="JQH65_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HAY6138125.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDSDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(17491..19626)
                     /gene="pnp"
                     /locus_tag="JQH65_000620"
     CDS             complement(17491..19626)
                     /gene="pnp"
                     /locus_tag="JQH65_000620"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAY6138126.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRSVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(19874..20143)
                     /gene="rpsO"
                     /locus_tag="JQH65_000621"
     CDS             complement(19874..20143)
                     /gene="rpsO"
                     /locus_tag="JQH65_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAY6138127.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(20292..21236)
                     /gene="truB"
                     /locus_tag="JQH65_000622"
     CDS             complement(20292..21236)
                     /gene="truB"
                     /locus_tag="JQH65_000622"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAY6138128.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICFGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITIYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(21236..21637)
                     /gene="rbfA"
                     /locus_tag="JQH65_000623"
     CDS             complement(21236..21637)
                     /gene="rbfA"
                     /locus_tag="JQH65_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAY6138129.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(21801..24449)
                     /gene="infB"
                     /locus_tag="JQH65_000624"
     CDS             complement(21801..24449)
                     /gene="infB"
                     /locus_tag="JQH65_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAY6138130.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(24474..25961)
                     /gene="nusA"
                     /locus_tag="JQH65_000625"
     CDS             complement(24474..25961)
                     /gene="nusA"
                     /locus_tag="JQH65_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAY6138131.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLELGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGIDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGVTDEKAGALIMAARNICWFGDEA"
     gene            complement(25989..26441)
                     /gene="rimP"
                     /locus_tag="JQH65_000626"
     CDS             complement(25989..26441)
                     /gene="rimP"
                     /locus_tag="JQH65_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAY6138132.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGHTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(26648..26724)
                     /locus_tag="JQH65_000627"
     tRNA            complement(26648..26724)
                     /locus_tag="JQH65_000627"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26688..26690),aa:Met,seq:cat)
     gene            27072..28415
                     /gene="argG"
                     /locus_tag="JQH65_000628"
     CDS             27072..28415
                     /gene="argG"
                     /locus_tag="JQH65_000628"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HAY6138133.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSNDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENMENKGQ"
     gene            complement(28371..30047)
                     /locus_tag="JQH65_000629"
     CDS             complement(28371..30047)
                     /locus_tag="JQH65_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HAY6138134.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFWAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKTFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISSAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMFRKEFTEDKQGNPPLKGRVDVELLAFVFHILHLRHA"
     gene            complement(30506..30592)
                     /locus_tag="JQH65_000630"
     tRNA            complement(30506..30592)
                     /locus_tag="JQH65_000630"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30556..30558),aa:Leu,seq:gag)
     gene            complement(30607..30939)
                     /gene="secG"
                     /locus_tag="JQH65_000631"
     CDS             complement(30607..30939)
                     /gene="secG"
                     /locus_tag="JQH65_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAY6138135.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(31167..32504)
                     /gene="glmM"
                     /locus_tag="JQH65_000632"
     CDS             complement(31167..32504)
                     /gene="glmM"
                     /locus_tag="JQH65_000632"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003905541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAY6138136.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIISKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(32497..33345)
                     /gene="folP"
                     /locus_tag="JQH65_000633"
     CDS             complement(32497..33345)
                     /gene="folP"
                     /locus_tag="JQH65_000633"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HAY6138137.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWLSVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(33435..35369)
                     /gene="ftsH"
                     /locus_tag="JQH65_000634"
     CDS             complement(33435..35369)
                     /gene="ftsH"
                     /locus_tag="JQH65_000634"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAY6138138.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMIEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(35469..36098)
                     /gene="rlmE"
                     /locus_tag="JQH65_000635"
     CDS             complement(35469..36098)
                     /gene="rlmE"
                     /locus_tag="JQH65_000635"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HAY6138139.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            36224..36517
                     /gene="yhbY"
                     /locus_tag="JQH65_000636"
     CDS             36224..36517
                     /gene="yhbY"
                     /locus_tag="JQH65_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAY6138140.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(36674..37150)
                     /gene="greA"
                     /locus_tag="JQH65_000637"
     CDS             complement(36674..37150)
                     /gene="greA"
                     /locus_tag="JQH65_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAY6138141.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            37398..38831
                     /gene="dacB"
                     /locus_tag="JQH65_000638"
     CDS             37398..38831
                     /gene="dacB"
                     /locus_tag="JQH65_000638"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HAY6138142.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMIATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVIPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
BASE COUNT         9439 a        10577 c         9574 g         9263 t
ORIGIN      
        1 tgacagcctg gatattaaaa ccacattaca ggcaaacgac attccaggac tcacaaccga
       61 ccaggttgtc tctgttaaca cccgattcac acagataaaa agcagcaccg tatattctgc
      121 tgcaacccaa acgggtgttt gccagggtga cacgtctcgt tatggacccg ttaatattgg
      181 tgcaaatacc acctttaccc tgtatgtcac caagccattt ctcggctcga tgaccattcc
      241 gaaaacggat attgccgtca ttaaaggcgc gtgggtcgat ggaatgggaa gcccgtctac
      301 aggtgacttc catgatttag tcaagttatc gattcaggga aatctcaccg ccccacagtc
      361 gtgcaaaatt aatcagggcg atgttattaa ggttaatttt ggattcatca atggtcagaa
      421 gtttaccacc cgcaatgcca tgccagacgg ttttactcca gtagactttg atatcactta
      481 tgactgtggt gatacttcaa agattaaaaa ctcgttgcaa atgcgcatcg acggtacaac
      541 tggggtagta gaccagtaca acctggtcgc caggcgaaga agttcagaca atgtgcccga
      601 tgtcggtatt cgtattgaaa atctcggcgg cggagttgca aatattcctt ttcagaacgg
      661 tatccttccc gttgatcctt ccgggcatgg caccgtcaat atgcgcgcct ggccagttaa
      721 tctggtcggt ggtgagctgg aaacaggaaa atttcagggc acagccacca ttaccgtcat
      781 ggtgcggtaa aagaagacgt tgcgttttga ttaacgcaac gcttcggcac tgttacccct
      841 gctgctccag cgcatactta tacagcgcat ttttcttcac gccatgaatt tctgcgacca
      901 gcgccgccgc ttttttcagc ggcagttctg cctgtagcag cgccagcgtg cgcagggcat
      961 cggcgggtaa gtcttcttcc tgtgctttat gaccttcgac aatcagcacc atttcgcctt
     1021 tgcgacggtt ttcatcttcc tttacccacg ccagcagttc gccaacgggc gcgccgtgaa
     1081 tggtttccca ggttttggtc agctcacgcg ccagaaccac gtagcgggat tcgcctaata
     1141 ccgcaacgat atcttccagg ctatctaaca gacggtgggt ggattcataa aaaatcagcg
     1201 tgcgcggctc cgcttcaatg gcttttagcg catcacggcg gccttttgat ttggcaggta
     1261 aaaagccttc gtaacagaaa cggtcagagg gtaaacccgc ggcgcttaac gcagtgatag
     1321 cagcacacgg cccgggtagc ggcaccacgc gaatccccgc ttcacggcag gtacgcacca
     1381 gatggtagcc aggatcgtta attagcggcg ttccggcatc ggaaaccagc gcaatgtttt
     1441 gcccctcttg cagcttcgcc agcagcgttt cggctttttg ttgttcgtta tggtcgtgca
     1501 gcgcaaacaa ccgggcatta atcccaaaat gttgcagcaa taaaccggtg tgacgagtat
     1561 cctcggcggc aatcagatca acggcctgta atacctctaa cgcacgctgg gtgatatccg
     1621 ccagattgcc gattggcgtc ggtacaatgt aaagctggcc ctgagaatta tccgccgatt
     1681 ggtgttgttt cattgtgtcg tccgtattgc cgatttaata ttgagcattg cgtaaaaaaa
     1741 tatcactgga tacattatgg taccctcaac attttctcgt ttgaaagccg cgcgttgtct
     1801 gcctgttgtt ctggcagccc tgattttcgc cggttgtggc acccatactc ccgatcagtc
     1861 cactgcttat atgcagggca cggcgcaggc tgattctgcc ttttatcttc agcagatgca
     1921 gcaaagctct gatgatacca ggatcaactg gcaattactc gccattcgtg cactggtgaa
     1981 agaaggtaaa accgggcagg cggttgagtt gtttaaccaa ctaccgcaag aactgaacga
     2041 ttctcagcgt cgcgagaaaa cactgctggc ggtagagatt aaactggcgc agaaagattt
     2101 tgctggcgcg caaaacttgc tggcgaaaat cacacctgcc gatttagaac aaaaccagca
     2161 agcgcgttac tggcaggcaa aaatcgatgc cagccagggg cgtccttcca ttgatttact
     2221 gcgcgcgtta attgctcagg aaccgctgct cggcgcgaaa gaaaaaaagc agaatattga
     2281 tgccacctgg caggcgctct cctccatgac tcaggaacag gcgaatacgc tggtgatcaa
     2341 cgccgacgaa aatattctgc aaggctggct ggatctgcag cgcgtctggt ttgataaccg
     2401 taacgatccc gatatgatga aagccgggat cgccgactgg cagaaacgtt atccgaacaa
     2461 tccgggcgcg aaaatgctgc caacgcagtt ggttaacgta aaagcgttta aaccagcctc
     2521 gaccaacaaa atcgccctgc tgttgccgct gaatggccag gcagcggtat ttggtcgcac
     2581 tattcagcaa ggctttgaag cggcgaaaaa tatcggcact cagccagtgg cggctcaggt
     2641 agctgccgca gacgtagcag aacaacctca gccgcaaact gcggatggcg ttgccagccc
     2701 ggcacaagcc tcggttagcg atctgaccgg tgatcagcct gcggcccagc cggtgcctgt
     2761 aagcgccccg gcgacaagca ccgcagcggt aagcgcaccc gcaaatccat ccgcagagct
     2821 gaaaatctac gatacctcat cacaaccact tagccagatc ttaagccagg ttcagcagga
     2881 tggcgcgagt attgtggttg gtccgttgct gaaaaataac gttgaagagt tgcttaagag
     2941 caacaccccg ctgaacgtgc tggtactgaa ccagccggag aatatcgaaa accgcgtcaa
     3001 tatttgttac ttcgcgcttt caccggaaga cgaagcgcgc gatgcagcgc gtcatattcg
     3061 tgaccagggt aaacaagcgc cgctggtgct gatcccacgc agttcattgg gcgatcgcgt
     3121 agccaatgcg tttgcgcagg agtggcagaa actgggcggc ggcaccgttc tgcaacaaaa
     3181 atttggttcc accagcgaat tacgcgcggg tgttaacggc ggttctggta tcgctttaac
     3241 gggtaccccg attactccca gagcgacaac cgactccggc atgacgacca acaatccaac
     3301 gctgcaaacc acgccaaccg atgaccagtt caccaataat ggcggtcgtg tcgatgcggt
     3361 gtacattgtg gcaacgccgg gtgaaatcgc ttttatcaaa ccgatgatcg ccatgcgtaa
     3421 cggtagccag agcggtgcaa tgctgtacgc cagctcccgc agcgcacaag gcaccgcagg
     3481 cccggatttc cgtctggaga tggaaggttt gcagtacagc gaaatcccga tgctggcggg
     3541 cggtaatctg ccgttaatgc agcaggcact cagcgcggtg aataacgatt attcactggc
     3601 tcgcatgtat gcgatgggcg tcgatgcctg gtcgctggca aatcatttct cacaaatgcg
     3661 ccaggttcag ggttttgaaa tcaacggtaa taccggaagc ctgacggcaa acccggattg
     3721 cgtgattaac aggaagttat catggctaca gtaccaacaa ggtcaggtag tccccgccag
     3781 ttaaccacca aacagaccgg cgatgcgtgg gaagtacaag cgcgtcgctg gctggaaggc
     3841 aaaggactgc gatttgtcgc cgctaacgtg aacgagcgtg gcggtgagat cgatctgata
     3901 atgcgtgaag gctggaccac cgtttttgtc gaggtgcgtt accgccgctc tgcgctttat
     3961 ggcggcgcgg cagccagtgt gacccgcagc aaacaacaca aattattaca gactgcccgc
     4021 ttgtggctcg cgcgtcataa tgggagtttt gatactgtgg attgccggtt cgatgtggta
     4081 gccttcaccg ggaatgaggt tgagtggatt aaggatgcct ttaatgacca ctcataatta
     4141 aggtttaagg attagcgtgc aagaaagaat taaagcttgc ttcactgaaa gcattcaaac
     4201 tcaaattgcg gcggcagagg cgcttccgga tgccatctcc cgtgcagcca tgacgctggt
     4261 tcagtctctg ctcaatggca acaaaatcct ctgttgtggt aatggaactt ccgctgccaa
     4321 tgcacagcat tttgctgcca gcatgatcaa ccgtttcgaa acggagcggc ccagcttacc
     4381 tgccattgca ctaaatactg ataatgttgt cttaacggcg attgccaacg atcgcttaca
     4441 tgatgaagtg tatgcaaaac aggtgcgggc gctgggtcat gcgggagatg tattgttagc
     4501 catttccacc cgtggcaaca gccgcgatat tgttaaagca gttgaagccg ccgttacgcg
     4561 tgatatgacc attgtggcat tgaccggcta tgacggcggc gaacttgcag gtttgttagg
     4621 gccacaggat gtggagatcc gcattccttc gcatcgtagt gctcgcattc aggaaatgca
     4681 tatgctgacg gtaaattgcc tgtgcgatct gatcgataac acgcttttcc ctcaccagga
     4741 tgattaagga gaatacatga aggcattatc gccaatcgca gtccttattt ccgcgctgct
     4801 gttgcaaggt tgtgttgccg ctgccgtagt gggtaccgct gctgtgggta ccaaagccgc
     4861 aactgaccca cgcagtgtcg gtacccaggt ggacgatggt accctggaag tgcgcgtgaa
     4921 cagcgcattg tcgaaagacg aacagattaa gaaagaaacc cgtattaatg tcacggcgta
     4981 tcagggcaaa gtgctgctgg ttgggcagtc accaaatgct gaactttcgg ctcgcgccaa
     5041 acagattgct atgggcgtag acggtgccaa cgaagtgtat aacgagattc gtcagggcca
     5101 gccgattggt ctgggcgaag catctaacga tacgtggatc accaccaaag tgcgttcgca
     5161 gctcttaacc agcgacctgg tgaaatcgtc caacgtgaaa gtgaccaccg aaaacggtga
     5221 agtgttcctg atggggctgg tgactgaacg tgaagcgaaa gcggcggcag atattgccag
     5281 ccgggtgagc ggcgtaaagc gggtaaccac agcgtttacg tttattaaat agcagcccct
     5341 tgtaatgcct gatgcgacgc ttgccgcgtc ttatcaggcc tacaacgatg caaaccgtag
     5401 gtcggataag gcgtttacgc cgcatccgac atgttttccc tcaaatcaga acaacagcgc
     5461 cagcccgccg acaatcacac cagacactgc caccatcgcc cctgtcagcc ataaggcttt
     5521 cgccgggaac gctttgcgca gcataatcag tgacggcaaa ctcactgccg ggagcgtcat
     5581 caacaatgcc agcgccggag cggttcccat acctgccagc atcatcgttt gtacaatcgg
     5641 aatttctgct gccgtgggaa tgacaaacaa gcatcctgct accgccatcg ccaccaccca
     5701 catcaggctg ttatcgacag caccatcggc atgggggaat aaccagacgc gtgcggcacc
     5761 caacaccagt actgcaagga tgtaaaccgg gatcgtactc cagaaaagtg tccatagcgc
     5821 cctgccccag cggctaaaaa aaccgctccg tgcttccggt atgtcaattt cgaccggtgc
     5881 ctgcgtttgc ggtgtttcac gcacccattt ttgcaccagc gtcgcaatca gcaacaccat
     5941 caccagcccg gccaccagac aaatcgccgc aaaaccccag ccgaggacaa accccataaa
     6001 caccagcgtc gccgggttta acaccggatt gcccatccag aatgccagcg caccgcccat
     6061 cgatacctgt tggcgacgca tccccgccgc gaccggagcc gcacagcagg tacacatcat
     6121 gcccggcagc gaaaacagcg ttcccagcag cgtgccgcga aagcgcgatt gcccaagcgt
     6181 acgcaacaac cagtcacgcg ggatcagcac ctgaatcaac gaaccgagga tcacccccag
     6241 caccgccgct ttccatactg cgaggaaata gatcatcgcg taatccaacg ccgcttgcca
     6301 cggattagca tccgcctgcg caaggataga tttaccgata ctgtgggttt cggctgcagt
     6361 aaacgctttg ccgtagtaat gttgccattt cacataccag agaccggcaa tgacaacgag
     6421 aaagaaaagc gcgggtttcc accactgaat gggtgttgcc gcctgagatg aagactgacc
     6481 agtcatagca ttccctagga ataagttatg ataattgagc gcgtgaatat tacgctcact
     6541 atcaattctt gggaataatt attcagctct tttgcgtaat tctgaagagc ttaaaatcgt
     6601 cacgccttca tgctcaggcc gcatcgcctc tgccagcatg acccgcgcca cgtctctcgc
     6661 atcaatggat ttccagttac ctggtaacaa acggaacagc ggcgcaaaaa gcgtttcgtt
     6721 catccgctgt ttgctacgat cgcccagtaa catcgacggg cgagcaatgg tcaatttcgg
     6781 ccagttctgg gcaattaatg cttcttccat ctcccctttg acgcggttat agaaaaacgg
     6841 cgagtgggca ttggccccca tagcactgac caccagcata tgctgcgcgc ccagccgccg
     6901 cccggttaat gcggtatcca ctaccagcgt gtaatcggca tgaataaacg cctctttgct
     6961 ccccgcttct cgccgcgtgg tgccgagaca acaaaacaca atgtcgatgg gttcggtgac
     7021 ctgcgccaac gcgtcactca gttgcggatc atggggatta aacaccccgg gcatatcgcc
     7081 caacggacgt cgcgtcggcg cagcaatggc gttaactttc ggttcattaa tcaacatccg
     7141 cagcaggtga ccgcccacca gccccgttgc gcctgtaatc agtacctgac tcatcttcgc
     7201 tcctttacag aattgtctgc cttgcgctcc acggctcatg caccagtctt aatagaccat
     7261 gaggtaatta tccccgattg tgggaaattc gcctcatcca atgcaacaac gcggaggaag
     7321 catgagtaag aaaattgccg ttttaatcag tgatgaattt gaggattcag aatttacttc
     7381 acccgcagac gagttccgta aagccggaca cgaagtaatt accattgaaa agcaagcggg
     7441 taaaacggtg aaaggcaaaa aaggagaagc cagcgtgacc atcgataaat ccatcgatga
     7501 agtgacgcca gcggagtttg atgccctgct gctaccgggc ggccattcac cggattatct
     7561 gcgtggggac aaccgttttg tcacctttac ccgtgatttt gtgaatagcg gcaaacctgt
     7621 tttcgccatc tgtcacggtc cgcagctgct gatcagcgcc gatgtgattc gggggcgcaa
     7681 actgaccgca gttaaaccga tcattattga tgttaaaaat gcgggcgcgg aattttacga
     7741 tcaggaagtc gtggttgata aagatcagct ggttaccagc cggacaccgg acgatctgcc
     7801 agcgtttaac cgcgaggcgt tacgcctgct cggtgcctga gtcgcgtagc caaatcattt
     7861 ttttaccaaa gcccagcgtg ttgtcggtga atttgatttc atcgagccgg atttcccaca
     7921 ccggggccga cagcattctg gcaaccggaa agcgatgatt gtacgccttg cgcgcgaggt
     7981 cgctttcctc accttccagc ctgcggatct cacctttaaa ctgcacaccg cgaattaacg
     8041 ctaccgtttt cggctgaccg tttaccgttc cggcaaccgc agcctgcggc ccactcatct
     8101 gcgcgtggcg cgttttttct tccgtcaaaa tgtagaaggc gactttctgc gcatcaaaca
     8161 gataaaaagc attagcgcac cacagttccc cttcctgctg cacacaccag gtgacaacat
     8221 gttgttttgc caaccaacgg ctgatggtga tgagtgtttc cattgctgtt ctcttttata
     8281 ctgtgggcct gaccttaaca tgcgacgcag acgatgacac cctggtttct ttacttgatc
     8341 cgtaccgccg acaataagct ttataccggg atcaccacgg atgtcgaacg ccgctatcag
     8401 cagcaccaaa gcggcaaagg ggcgaaagca ctgcgcggga aaggagaact gacgctggcg
     8461 ttttctgcgc cagtgggcga ccgttcgctg gcactacggg cggaatatcg cgttaagcaa
     8521 ctgaccaaac ggcaaaaaga gcgtctggta gcggaaggcg cagggtttgc agaactgttg
     8581 agcagcctgc aaaccccgga gattaaaagc gattgaaatg ctcgtgatac tcaaccagac
     8641 cggttacgcc attcagcgcg tcatccgcta aacgatgtac ctggaaggcg ctttcagtgc
     8701 ccggccagcg gcaacgcaga tcgtgatgcg ccgccagttc aaagccgaaa cgactgtaca
     8761 gcgccggatc gcccagcgtc accactgcgg catagccgaa ctcattaagc gaatcgagtc
     8821 cttcatagac cagttggcgt gccagcccct gtccacggta tttttcatca accgccaacg
     8881 gtgccatgcc gacccattgc aggtcttcgc cctgcacatc aaccggacta aatgccacat
     8941 agccaatgac ctgaccttcg tcatctgtcg ccaccagccc cagcgtcaga aagccatctt
     9001 cacgcagatc gtgaaccagc ttcgcttccg catcactttc gaatgagcga cgcagcaggg
     9061 catcaatacc cggcgcatca atgggaattt ctactcgaat tagcatggtt cacctaccga
     9121 tgtctgtttg gtttcaggcg tggttttcat tcccgcctca acaaaatccg ccagttgcag
     9181 cagcatcatg cgtaaggctt tcggcatttg ctccagttca atggcgtcca tcaggttttt
     9241 cacatgcagc cccagctccg tatcgccttc aatcaccagc cgacgctgga agaagagcgt
     9301 atctggatct tgtttacgcg ccgcaatcat cagcagatcg ctgacgtcgg cactaaaact
     9361 cacatcagct tgcgcgttct ggctgaccac cagtttgcca ttcaccaccg aggtaaacca
     9421 ttgcaggtca atatcacgca catgaatact taaccagcgg ccttcaagaa actccagctc
     9481 gccatcatcc agcgcctgac ggaattgcca gcttaagacc tgctcaagaa cctggcgttt
     9541 tagcgcaaat ggcgtcagtt ttaccggtac actcaacaga gatggcccca aatgcacaat
     9601 acgggaacga agtttatcca acacgagttt tactccctgt ttcaacaatc atcctatttt
     9661 gccatatcag aaaaataaca tagcggtata aatcaacaat tccatatgaa attgctgcta
     9721 ccaccaatac aactttaact gccttaaatc aaaaattgtc gcagcaaggt taactaaaat
     9781 cccagttcgt taacattttt gcgttttgat agcgcaacct tcaggaaaaa ttatggagct
     9841 gctctgccct gccggaaatc tcccggcgct taaggcggcc atcgaaaacg gcgcagatgc
     9901 tgtttatatc gggctaaaag atgataccaa tgcccgtcac ttcgccggcc ttaactttac
     9961 cgagaaaaaa ttgcaggaag cggtgagttt tgtccatcaa catcgccgca aacttcacat
    10021 cgcgattaac acttttgcgc atccggacgg ttacgcccgt tggcagcgcg ccgtggatat
    10081 ggcggcgcag ctgggtgccg acgcgctgat cctcgccgac ctcgccatgc tggaatacgc
    10141 cgccgagcgt tatccgaata ttgagcgcca cgtatcggtg caggcttcgg cgaccaatga
    10201 agaggcgatt aacttttatc atcgccattt tgacgttgcc cgcgtggtgc tgccgcgcgt
    10261 gttgtcgatt catcaggtga aacagctggc acgggtcaca cctgtaccac tggaagtgtt
    10321 tgctttcggc agcctgtgca ttatgtcgga aggccgttgc tatctgtcgt cgtatctgac
    10381 gggtgagtcg cctaacaccg tgggcgcgtg ttctccggcc cgtttcgtgc gctggcaaca
    10441 aacgccgcag gggctggaat cccgcctgaa cgaagtgctg atcgaccgtt atcaggacgg
    10501 cgaaaacgca ggttatccga cgctgtgtaa agggcgttat ctggtggacg gcgagcgcta
    10561 tcacgcgctg gaagaaccaa ccagtctcaa taccctggaa ctgctgccgg agttaatggc
    10621 ggcgaatatt gcttcggtga aaattgaagg ccgccagcgt agcccggcgt atgtcagcca
    10681 ggtggcgaaa gtctggcgtc aggctatcga ccgttgtaag gccgatccgc aaagcgcgtg
    10741 gatggagacg ctcgggtcga tgtccgaagg cacgcagacc acccttggcg cgtatcaccg
    10801 taaatggcag tgagaagagc aatgaaatat tccttagggc cagtgctgtg gtactggcca
    10861 aaagagacgc tggaagattt ttatcagcag gccgccacca gcagcgccga cgtgatttat
    10921 cttggtgaag cggtatgcag caagcgccgg gcaaccaaag ttggcgactg gctggagatg
    10981 gcaaaatcgc tcgccggaag tggcaagcag attgtgctct ccacgctggc gctggtgcag
    11041 gcatcctctg aactgggcga actgaaacgc tatgttgaga acggtgagtt tctgattgaa
    11101 gccagcgatc tcggcgtggt gaatatgtgc gctgaacgca aactgccgtt cgtcgccggg
    11161 cacgcgctga actgctacaa cgcggtgacg ctgaaaatat tgctcaaaca gggcatgatg
    11221 cgctggtgta tgccggtgga gctttcccgc gactggctgg tgaatctgct taatcagtgc
    11281 gatgagctgg gcattcgcaa ccagtttgaa gtggaagtcc tgagctacgg tcatctaccg
    11341 ctggcctact ccgctcgctg ctttaccgcg cgttcggaag accgcccgaa agatgagtgt
    11401 gaaacctgct gcattaagta tccgaacggg cgcaacgtgc tgtcgcagga aaaccaacaa
    11461 gtgtttgtac tcaatggcat tcagaccatg agcggctacg tttacaacct cggtaacgag
    11521 ctgacatcca tgcagggatt ggttgatgtg gtccgcctgt caccgcaggg tactgacact
    11581 ttcgcgatgc tcgacgcctt ccgcgctaat gaaaatggcg ctgcgccact attgttgacg
    11641 gcaaacagcg actgtaacgg ctactggcgg cggctggcag gactggagct gcaagcctaa
    11701 gtaaatatgc tcgtcatact tcaagttgca tgtgctgcat ctgcattcgt tcaccccagt
    11761 cacttactta tgtaagctcc tggggattca ctctcttgtc gccttcctgc aactcgaatt
    11821 atttagagca cagctcactt tgttaacaac tttaactact ctttaatgca gtattaaaga
    11881 ttaatcggta acaaagtgag ctgttatgac tgataaaacc attgcgtttt cgttactcga
    11941 tctggccccc attcccgaag gttcttcagc gcgagaagca ttctcccact ctctcgatct
    12001 cgcccgtctg gctgaaaagc gcggctatca tcgctactgg ctggcagaac accacaatat
    12061 gactggcatt gccagtgctg ccacgtcggt attgattggc tatctggcgg cgaataccac
    12121 cacgctgcat ctggggtctg gcggcgtgat gttgcctaac cactcaccgt tggtcattgc
    12181 cgaacagttc ggcacgctca atacactcta tccggggcga atcgatttgg ggctgggtcg
    12241 tgcgccaggt agtgaccaac ggacaatgat ggcgctgcgt cgccatatga gcggcgatat
    12301 tgataatttc ccccgcgatg tcgcggagct ggtggactgg tttgacgccc gcgatcccaa
    12361 tccgcatgtg cgcccggtac caggctatgg cgagaaaatc cccgtgtggt tgttaggctc
    12421 cagcctttac agcgcgcaac tggcggcgca gcttggtctg ccgttcgcgt ttgcctcaca
    12481 cttcgcgccg gatatgttgt tccaggcgct gcatctttat cgcagcaact tcaaaccgtc
    12541 ggcacgactg gaaaaaccat acgcgatggt gtgcatcaat attatcgccg ccgacagcaa
    12601 ccgcgatgcc gaattcctgt ttacctcaat gcagcaagcc tttgtgaagc tgcgccgcgg
    12661 cgaaaccggg caactgccgc caccgattca aaatatggat cagttctggt cgccgtccga
    12721 acagtatggt gtgcagcagg cgctgagtat gtcgttggta ggtgataaag cgaaagtgcg
    12781 tcatggcttg cagtcgatcc tgcgcgaaac cgacgccgat gagattatgg tcaacgggca
    12841 gattttcgac caccaggcgc ggctgcattc gtttgagctg gcgatggatg ttaaggaaga
    12901 gttgttggga tagtgtgtct taacgcggga agccttatcc gagctggcaa cgctgtccta
    12961 cgtagacctg ataagcgaag cgcatcaggc attgtgtagg cagcagaaat gtcggataag
    13021 gcaccgctga ttactgatac accggcagta aattaaagct cgataaaata tgtaccaatg
    13081 cgttgccgac gccaaacacc agaatcagcg taatcatcgg cttgccgccc cagacgcgga
    13141 atttcgggct gccaaagcgt ttacgcgatg cgcgggccag cagtgccgga acaattgccg
    13201 cccagatggt agccgctaaa ccagcataac caatggcgta caggaatcca ttcgggaaca
    13261 gcagaccacc cacaacaggc ggtgcaaagg tcaacaatgc tgttttcaag cggcccatag
    13321 ccgagtcatc gaaaccaaac agatctgcca gatagtcaaa caaacccagc gttacgccaa
    13381 ggaacgaact cgctaccgca aagtttgaga acacgaccag cagaaggtcc aggctacggc
    13441 tgttcagcac gccgcttaat gcctgtacca gcacatcaat attaccgccc ttctctgcaa
    13501 taccgataaa ctccggacgc gggatgttac ccatcgtcgc cagcaaccag atggtataca
    13561 gcgccagtgc catcagcgta ccgtacacca ggcatttcac gatggttttc ggatctttgc
    13621 cgtaatactt catcaggctt ggcacgttac cgtgataacc aaacgacgcc aggcagaacg
    13681 gcaatgtcat cagcagatac ggcgcataag atgcattgct ttctgcaacg ttgaacaatg
    13741 tcgcaggctg cacatgcccc aacaggctgc caaaggtgag gaagaaggta atgactttcg
    13801 cccccagcac aatcgccgtc atgcggctga cggccttagt actcaaccac accacaaacg
    13861 ctaccagcag tgcaaaacca aaacccgccg cccgtgccgg gacgtttagc gacatctctg
    13921 cgaaggtgtg atgcagaatt gaaccactgg cagaaatata ggcataggtc aggatataga
    13981 gcacaaaggc aatggaaata ccgttgacca cgttccagcc tttgcccagc aaatctttgg
    14041 tgatggtgtc aaaactcgaa ccgattcggt aattcaggtt agcttccaga atcatcaagc
    14101 cggaatgcag catacagaac caggtaaaga tcagcgccgc cattgaccag aaaaaccacg
    14161 ccccggacat gaccactggc agagaaaaca tccctgcgcc aataatggtg ccgccgataa
    14221 tcaccacgcc gccaagcagc gacggtgacg tttgggtggt ggttagtgtt gccatgaggg
    14281 cttctctcca gtgaaaaata gtgcgactgc gttgttatgc attgcactgt accagtacgc
    14341 gagtacaaaa gacagaaaaa aagccccgat ggtaaaaatc ggggctgtat atattatttt
    14401 acagattgtg ttcgctgttc agcgatgatt acgcatcacc accgaaacga cgacgaccgg
    14461 tagagtcatc acgacgcgga gcgcggcctt cacgacgttc gccgctaaaa cgacgaccat
    14521 caccacggcc accttcacgg cgttcaccgc tgaagttacg accgccttca cgacgttcgc
    14581 caccgaaacc acgaccaccg ccacgacgct caccgccagt atgcggctgt gcatcgccca
    14641 gtaactgcat gttcatcggc ttgttgagaa tgcgagtgcg cgtaaagtgt tgcagcactt
    14701 cacccggcat acctttcggc agttcgatgg tggagtgaga agcaaacagc ttgatgttac
    14761 caatgtaacg gctgctgatg tcgccttcgt tagcaatcgc accaacgata tgacgaactt
    14821 caacaccatc atcacggccc acttcaatgc ggtacagctg catatcgcca acatcacgac
    14881 gttcacgacg cggacgatct tcacggtcac cacgcgggcc acggtcgtta cgatcgcgcg
    14941 gaccacggtc atcacggtca cggaattcac gtttcggacg catcggcgca tctggcggta
    15001 cgatcagagt acgttcaccc tgtgccattt tcagcagtgc cgcagccaga gtttcgagat
    15061 ccagctcttc accttctgca gtcggctgaa ttttgctcag cagtgcgcgg tattgatcca
    15121 gatcgctgct ttccagctgc tgctgtactt tagcggcgaa tttttccaga cggcgtttgc
    15181 ccagcagttc tgcgttcggc agttctactt ccggaatagt cagcttcata gtacgttcaa
    15241 tgttgcgcag cagacgacgc tcgcggttct caacgaacag cagcgcgcgg ccagcacgac
    15301 ccgcacgacc ggtacgaccg atacggtgaa cgtaagactc agaatccatc gggatatcgt
    15361 agttaactac caggctgata cgctcaacgt ccaggccacg ggctgcaacg tcggtcgcaa
    15421 tcaggatgtc cagacgacca tctttcaggc gttccagtgt ctgttcacgc agcgcctggt
    15481 tcatgtcacc gttcagcgcg gcgctgttgt agccgttacg ctcaagagct tcagccactt
    15541 ccagagtcgc gtttttggta cgaacgaaga taatcgccgc atcaaaatct tccgcttcca
    15601 ggaaacgtac cagtgcttcg tttttgcgca taccccagac agtccagtag ctctggctga
    15661 tgtcaggacg ggtagtcacg ctggactgaa tgcgcacttc ctgcggctct ttcataaagc
    15721 ggcgggtaat gcgacgaatc gcttccggca tggttgcaga gaacagagcg gtctgatgac
    15781 cttccgggat ctgcgccata atggtttcaa cgtcttcgat gaagcccatg cgcagcattt
    15841 cgtcagcttc atccagaacc agaccgctca gtttagagag gtccagagtg ccacgtttca
    15901 ggtggtccag cagacggccc ggagtaccga caacgatctg cggcccctga cgcagggcgc
    15961 gtaattgcac gtcataacgc tggccgccgt acagagcaac cacatttacg ccgcgcatgt
    16021 gtttagagaa atccgtcatt gcttcagcaa cctgtaccgc cagttcgcgg gtcggtgcca
    16081 gcaccagaat ctgtggtgct ttcagctcag gatcaagatt ctgcaacaga ggtaaagaga
    16141 atgctgcagt ttttccgctc cccgtctggg ccatacccag aacgtcgcgg ccattcagca
    16201 gatgtggaat acactctgcc tgaattggag atggtttttc gtaacccaga tcgttaaggg
    16261 cttcaaggat aggagccttc aggcccagat ctgcaaaagt ggtttcgaat tcagccatgt
    16321 agtacgtgtg cctcaaaatt aatggcggcc agtctacata actcatcatg aaattgatca
    16381 gcaattttca ttgaaaagtg tgaaccggct caaagtaggt gtattaacga acaacaacgc
    16441 cctcacccct taaggtgatg gcaatcaaaa aagattacgg gctgatgtgt acgtcagcta
    16501 ttgctggtcc gattctgcca ggtcatcttg gtcctggccc aggagcgata attccaacaa
    16561 tgcgtatcgg tgctcaacaa agttatgaac gttgttggca accgccagtt tgaacagtgc
    16621 cgtggcgctg tccgaatccc ccagacttag gtagtactta cctaaataga agttggtttc
    16681 actgagatgc tcagcgagcg aggtgttatc cgttgcgtcc gccttgagcc tttccattaa
    16741 cgtttgttcg ctaatgttgc ccaggtagaa ctcgacaatg ttccatcccc actgttcctt
    16801 atccgatttt tcgaagtgct gtttcaacac ttctttagcc tgcttctcat cgagcttctg
    16861 ctcggcgaga taaagccaca gactacggaa aggatcattg ggatcgtctt gataaaacgc
    16921 cagcagatca tcttgcgcta acttgtcacg accgccgtaa tataatgcga tcccgcgatt
    16981 caagtgcgcg tagttgtaag ttggatcaag ctcaagtaca gaatcaaacg cttcataggc
    17041 agcatcaaaa ttgcctgcct gcgttaaata tatgcctaag taattgaaca cttcaggcat
    17101 atccggtcgg attgccagcg cttgcgaaaa atcgttacgc gctaatgccc tcagaccgag
    17161 actatcatac aacactccgc gctcatataa aagctgtgcg cgttcgtcat cggttaaagc
    17221 ccgactggca aggatttgtt ccatacgtgc cagaatcact tcctgctgta aagtcggttg
    17281 caatggtacc gcgaggactt cacttttacg ccaggaagta ttactgcatc ctgcaagcgt
    17341 aagtgctgtc gcaacgaaac accagcgcaa aaaaggcttc atttcccact cccgaagacc
    17401 acggttgaat gaacgtcctg ttcccggttg ctaacaaggc gtcctgcccg gttaaaagcc
    17461 ccccgccgca gcggagggca aatggcaacc ttactcgccc tgttcagcag ccggagcttc
    17521 cggtgctgca gcaggttgag actgctcagt cgcttcttta atgctcagac ggatacggcc
    17581 ctggcgatca acttccagaa ctttcaccgg tacttcctga cccatctgca ggtaatcggt
    17641 cactttctca acgcgtttgt cagcgatttg agagatgtgg accagacctt ctttaccgcc
    17701 gccgatggca acaaatgcgc caaagtcaac gatacgggtc actttaccag tgtagacgcg
    17761 gcccacttcg atttctgcag tgatctcttc gatacgacga atagcatgtt tcgctttctc
    17821 gccgtcggtc gctgcgatct tcacagtacc gtcatcttcg atttcgatgg tagtgccagt
    17881 ttcttcggtc agggcacgga taacagagcc gcctttaccg ataacgtctt tgatcttgtc
    17941 cgggttgatc ttgatggtat ggatacgcgg tgcgaactca gagatatcgc cacgcggcgc
    18001 gttgatcgcc tgttccatta cgcccaggat atgcagacgc gcacctttag cctggttcag
    18061 cgcaacctgc atgatctctt tggtgatacc ttcaattttg atatccatct gcaatgcaga
    18121 gataccctcg cgggaacccg caactttgaa gtccatatcg cccaggtgat cttcgtcgcc
    18181 caaaatgtca gacagtacaa cgtagttgtc gccttctttc accagaccca ttgcgatacc
    18241 cgcaacggca gctttgatcg gcacacctgc gtccatcagc gccagagacg cgccgcacac
    18301 ggaagccata gaagaggaac cgttggattc agtgatttca gacacaacac gtacggtgta
    18361 cgggaatttg tccatatccg gcatgactgc cagcacgctg cgcttcgcca gacgaccgtg
    18421 accaatttca cgacgcttcg gagaaccgac catgccggtt tcgcctacgg agtacggagg
    18481 gaagttgtag tggaacagga aggtatcggt acgttcgccc atcagttcat caagaacctg
    18541 cgcgtcacga gcagtaccca gcgttgcggt aaccagcgcc tgcgtttcac cacgggtgaa
    18601 cagcgcagaa ccgtgagtac gcggcagcac gccagtacgc acatccagac cacggatcat
    18661 atctttttca cgaccgtcga tacgcggttc gcctgccagt acgcggctac gaacaacgtt
    18721 tttctcgata gcgtgcagaa tttcacccag ttcgttttcg tccagggttt cgtcttcagc
    18781 aagcagcgtc gcgatggttt cagatttgat gacatcaacc tgcgcataac gctcttgttt
    18841 gtcggtgatg cggtaagcat cgctcaggcg agcttcagcc agtgcagcaa cgcgcgcgtt
    18901 cagcgcttcg tttaccggct ccggctgcca gtcccaacgt ggtttaccgg cttctttcac
    18961 cagttcattg atgttctgaa taacaacctg ctgttgttca tgaccgaaca ctactgcgcc
    19021 cagcatctgg tcttcgctca gcagttcagc ttcagattca accatcagaa cagcggcttc
    19081 agtaccggca acaaccagat ccagtttgct ctctttcagc tcgtcctgag tcgggttcag
    19141 tacgtactgg tcattgatgt aacctacgcg agcagcacca atcggaccat tgaacggaat
    19201 accagacaga gacagtgctg cggaagcacc aatcatcgcg acgatatccg ggttaacttg
    19261 cgggttaaca gaaaccacgg tggcgataac ctgaacttcg ttgacgaagc cttccgggaa
    19321 cagcgggcga atcgggcggt caatcagacg cgcgatcagg gtttcgcctt cgcttggacg
    19381 accttcacga cggaagaagc tacccgggat acgaccagca gcgtaggtac gctcctgata
    19441 gttaacggtc agtgggaaga agtcctgacc tggtttggct tttttctggc caacaacggt
    19501 aacaaatacc gcggtgtcat ccatgctaac cataacagcg gcagtagcct gacgagccat
    19561 catgccggtt tccagagtca cggtgtgttg gccgtactgg aatttacgaa cgatcggatt
    19621 aagcaatgta atatcctttc tctttcttag acagtacctt acggcactgg tgttaatacc
    19681 cgatcttctg cgcatcctcg cgactaatga caaccctaac ccagctctat gtgggtaaag
    19741 cctctcatta gccgcgcgaa cctctgcaac ggaagatcat tcatagcaac aatacattag
    19801 tttccagtga attgctgccg tcagcttgaa aaaaggggcc actcaggccc ccttttctga
    19861 aactcggcag gaattagcga cgcagaccca ggcgctcgat gagctgggtg taacgtgcta
    19921 cgtctttacg tttcaggtag tcgagcagtt tacgacgctg agaaaccatg cgcagcagac
    19981 cacgacggct gtggtgatct tttttgtgct ctgcaaagtg gccctgcagg tggttgatct
    20041 gtgcagtcag cagtgctacc tgaacttcgg tagaaccggt gtcgtttgcg tcacgaccaa
    20101 actcagaaac gattttagct gttgcttcag tacttagaga cattttaaaa ctccaaagta
    20161 tatagaatga aaggacgccg atctctaatt cagcgatccc agtgtacgtt acgcaaagtg
    20221 ttaaacaatt tacgcgacgt taagcggcag tattctactc gtagcgactt gttatcgcaa
    20281 gacggttaac attacgccgg gtattcaacc accaggcgac gaggcgcgac gcggccttca
    20341 tcgtcaattt cgcccatacc gataaacttg ccgttctcac cttctgtaac gcgaaccagt
    20401 ccttccagcg gcgcaccgga tgtacgaact gggttaccat ttttgaagta aacagaagac
    20461 gttaacggaa gattcaccac cgggtagtcc gaagctggac tgtccattgg catcagtaat
    20521 ggatcaagta actccgcagc cggaatatcc tgctgttcgg cttgttcgac cagttcacgc
    20581 agatgttcca gggtcaccat ccgttcaacc ggatatttac ttaccgccag acgacgcagg
    20641 taaataacat gcgcgccaca accgagtttt tcacccaggt catcaatgat ggtgcggata
    20701 taagtgcctt ttgagcagtg aatttccagc tccagctcat tgccttcgtg gcgaataaac
    20761 agcaattcat aaatggtaat cgggcgcgct tcacgcggta cttcaatgcc ctgacgcgca
    20821 tattcgtaca gttttttacc ctgatatttc agtgctgaat acatcgaagg gatctgttcg
    20881 atatcgccac ggaaagtatc cagcgccgcc gccagttgct ctgcgctaaa ggttaccgga
    20941 cgttcttcaa cgatttgtcc gtcggcatca gaagtatcgg tacgctgtcc aagacgcgca
    21001 atgacccgat agcgtttgtc ggagtccagc agatactggg aaaacttcgt cgcttccccg
    21061 aagcaaattg gcaacatgcc agttgccagc gggtccagcg caccggtatg cccggcacgg
    21121 ttggcgttat aaatccgttt cactttttgc agcgcatcat tgctggacat accctgcggt
    21181 ttatccagca acaaaacgcc gttaatgtcg cgaccgcgac gacgaggacg actcattagt
    21241 cctccttgct gtcgtccggg ttaacacgac gttcttcgtc atgtttgacc acgctggtca
    21301 ccaggtttga catgcgcatc ccttcaacca gagagttgtc gtagaagaag gtcagttccg
    21361 gcacgatacg caggcgcatc gctttcccca gcaggctgcg gatgaaacca gaagcttctt
    21421 gcaacgcttt gatgcccgct ttaaccgcgt cttcatcttt gtcgttgagg aacgtcacat
    21481 atactttggc atacgccagg tcgcgagaca tttcgacacc ggaaacggtg gtcatcatgc
    21541 ccaggcgagg atctttaatt tcacgctgca ggatgagagc gatctctttt tgcatttcct
    21601 gcgctacgcg ctgcgggcga ccaaattctt tcgccataat aaattctcct gacaaaaaag
    21661 ggactgttag ccccttttta aaattaattt caggtggaag ggctgttcac gttggcctga
    21721 taagacgcgc cagcgtcaca tcaggcaatc catgccggat gcggcgtaaa cgccttatcc
    21781 cgcatggaac cctaaaaacc ttaagcgatg gtacgttgga tctcgatgat ttcgaatact
    21841 tcgatcacat cgccagtgcg gacgtcgttg tagttcttaa cgccgatacc acattccata
    21901 ccgttacgga cttcgttaac gtcatctttg aagcggcgca gggactccag ctcgccttcg
    21961 tagataacca cgttgtcacg cagaacgcgg atcgggttgt gacgtttaac cacaccttcg
    22021 gtaaccatac agcctgcgat ggcaccaaat ttcggcgatt tgaacacgtc acgaacttcc
    22081 gccagaccga taatctgctg tttcagttcc ggagacagca taccgctcat cgccgctttc
    22141 acttcgtcaa tcaggttata gatgacggag tagtaacgca gatctaggct ttccgcttca
    22201 atcactttac gtgcagaggc atcagcacgt acgttgaagc caaccaggat ggcgttggac
    22261 gccgcagcca gggtggcgtc ggtttcggtg ataccaccta cgccagaacc gatgatcttc
    22321 actttaactt cgtcagtaga cagtttcagc aaggagtcgg agatcgcttc cacagaaccc
    22381 tgtacgtctg ccttcaggac gatgttcact tcgtgaactt cgccttcggt catgttggcg
    22441 aacatgttct cgagtttaga tttctgctga cgcgccagtt taacttcgcg gaatttaccc
    22501 tgacgataca gtgcaacttc acgcgctttc ttctcgtcac gtacaacggt aacttcatca
    22561 cccgcagccg gtacgccgga caggccgagg atttccaccg gaatggacgg acccgcttcc
    22621 agcacttcct gacccagttc gttacgcatc gcacgaacac gaccgtattc gaagccacac
    22681 agaacgatat cgcccttgtg cagagtacct tcacgtacca gaacggtagc aaccggacca
    22741 cgacctttat cgaggaagga ttcgataacc gcaccgctcg ccataccttt acgtaccgct
    22801 ttcagctcca gaacttccgc ctgcagcagg atagcgtcca gcagttcatc gatgccggta
    22861 cccgctttcg cagatacgtg tacgaactgg ctttcaccgc cccactcttc tggcaggatg
    22921 ccatactggg agagttcgtt cttaacacga tccggatcgg cttctggttt atcaatcttg
    22981 ttcactgcaa ccaccaccgg tacctgcgcc gctttcgcgt gctggattgc ttcgatggtc
    23041 tgcggcatca cgccgtcgtc ggcagcaaca accaggacta cgatgtccgt tgcctgcgca
    23101 ccacgagcac gcattgaagt aaacgcggcg tgccctgggg tgtccaggaa ggtgatcatg
    23161 ccgttttcag tttcaacgtg gtatgcacca atgtgctggg taatgccgcc cgcttcgcca
    23221 gaggccactt tcgttgaacg aatgtagtcc agcagagagg ttttaccgtg gtcaacgtga
    23281 cccatgatgg tcacaaccgg cgcgcgcggt tcagccgcag cccccgtgtc acggtcgctc
    23341 attaccgcct cttccagctc gttttcacga cgcaggataa ctttgtggcc catctcttca
    23401 gcaaccagct gtgcggtttc ctgatcgata acctggttga tggttgccat tgcgcccagt
    23461 ttcatcatcg ctttgatgac ctgagagcct ttaaccgcca tcttgttcgc cagttcgcca
    23521 acggtgatgg tttcgccgat cacaacgtca cggttaacgg cctgagctgg cttctggaag
    23581 ccttgctgca gggaagaacc tttacgtttt ccgcctttac ctccacgtac tgctgcgcga
    23641 gcttcttcac gatcagcttt tgattcagcg tgtttgttgc ctttcttcgg acgcgctgct
    23701 ttcgcgttac gaccacggcc acggccgcct tcgacttcac gatcgctttc gtcttctgcc
    23761 tggcgagcat gttgagaagt agtgacgtga taatcgctgg aatcttcagt cggttccgcg
    23821 ttatcagtcc atttgttttc ttccgccata cgacgtgctt cttcagcaac gcgacgtgct
    23881 tcttcttcga gtttacgacg cgcttcttct tcagctttac gcttgagctc tgcagcttcc
    23941 tgctcacggc gggctttttc agcctgggcg tttttagtca tatcgtcttg ttgattgctc
    24001 actttgtctt tttccgcagc ttcacgtttc gcttgttcag cagcttcacg cttagcttgt
    24061 tctgcggcct cacgttcagc tttttgttgc gcctcgcgtt tagccgattc ttctgcctca
    24121 cgacgggctt gctcttccgc tgcaaggcgt tcagcctctt gcggatcgcg tttcacaaag
    24181 gtgcgtttct tgcggacttc gatttgtacc gatttgcttt ttccaccggt accaggaatg
    24241 ttaagggtgc tgcgtgtttt acgttgcagc gtcaatttgt ccgggcctga atttttctga
    24301 ttcaggtggt caatcaaagt ctgtttctct tgtgcggaca cagagtcgtc agcagacttc
    24361 cggatacctg catcagcaaa ttgctgtacc aggcgttcca cggaggtctg tcgctctgcg
    24421 gccagcgttt taatcgttac atctgtcatg ctgttccttc ctgctacagt ttattacgct
    24481 tcgtcaccga accagcaaat attacgggca gccataatca gtgctccggc tttttcgtcg
    24541 gtcacccctt cgatatcagc cagatcatca atgccctgtt cggcgagatc ttccagcgta
    24601 caaacgccac gggcggccag tttgaatgcc aaatcacgat ctatcccttc aaggttcagc
    24661 agatcgtcag ccggtttgtt atcaccgagg ctttcttcct gggcctgtgc aatggtggcc
    24721 agtgcatttt tagcacgctc gcgcagtgct tcaacggtcg gctcatcaag gccttcgatt
    24781 tccaacagct ctttcatcgg cacataggcc agttcttcca gcgtcgagaa gccttcttct
    24841 accagaacag tcgcgaagtc ttcgtcgatg tcgagatatt tggtgaaggt gtcgatcgct
    24901 gcgtgcgctt ccgcctgatg cttagcctgc aggtcgtcaa cggtcatcac gttgagttcc
    24961 caaccgctca gctgcgaagc cagacgcacg ttctgaccgt tacggccaat cgcctgcgcc
    25021 aggttaccgg cttcaacggc gatatccatg gtgtgtttat cttcatccac cacgatagaa
    25081 gcaacgtctg ccggtgccat tgcgttaatc acaaactgcg ccgggttatc atcccacagg
    25141 acgatatcga tacgctcgcc gcccagttca gtagaaaccg cctgaacacg cgcgccacgc
    25201 atacctacac aggcacctac cggatcgata cgtttatcgt tggttttcac cgcgattttc
    25261 gcacgagaac ccggatcgcg agccgctgct ttaatttcaa tcacttcttc gccgatttct
    25321 ggcacttcaa tacggaacag ttcgatcagc atttccggct tggaacgagt gacgaacagt
    25381 tgcgcgccac gcgcttccgg gcgaacggaa tagagcacgc cacgaacgcg gtcgccaggg
    25441 cggaagtttt cacgcggcag catatcttcg cgcaggatca cggcttcagc attgttgccc
    25501 agctccagag agatgttgtc gcggtttact tttttcacca cgccggtgat gatttcacct
    25561 tcgtgttcac ggaactgatc aaccaccatc gcacgttcgg cttcacgcac tttctgcacg
    25621 ataacctgtt ttgccgtctg ggtagtgata cggtcaaagg taacagactc aatctgatct
    25681 tcaacgtaat cgcccaggtt caggctttca tcttcataac gtgcggcttc aagggtgatt
    25741 tccttggtcg gctgggtgac ttcatcaaca actaaccagc gacggaaggt gtcaaaatca
    25801 ccgcttttgc gatcgatctg tacgcggacg tcgatctctt gttcatattt tttctttgtt
    25861 gctgtcgcca gcgcgctttc caatgcttcg aaaatcttct cgcgaggtag cgccttttca
    25921 ttggatacgg cttcaactac agccaaaatt tctttgttca tcgcgggctt ttcacctcat
    25981 ccagactatt aaaagtgggg aaccaggttc gccttctgga tattactcag cgcgaacact
    26041 tcatctttac cttcgacggt aactgtgatc atttcaccgt ctaccgcttt gataacgccc
    26101 tgccatttac gacggttttg taccgccata cggagaacca gagtcacctc ttctccgaca
    26161 aaacgggcgt agtgttcagc cgtgaacagt gggcgatcga gacccggtga ggagacttcc
    26221 aggttataag caacggtgat gggatcttca acatccagca cagcacttac ctggtggctc
    26281 acatcagcac aatcatcaac attgatgcca tcttcactat caatatagat gcgcagtgtg
    26341 gatgtgtgac cgcgaataaa ttcgatgcca accagttcaa aacccagggc ctcaactggc
    26401 gcagtaatca tctctgttaa tttttgctct aatgtggaca agcccacccc caagacataa
    26461 aaaaagggcc taaagcccag ttattctgta gtcagataac aaaaaacccc gataaatcgg
    26521 ggctttatat aactgaaccc tataatcgca actgcggtct ggagcacttt ccagaaggat
    26581 tttttcaaat cccactacga aggccgaagt cttcacagta tatttgaaaa aggactctaa
    26641 gggaaagtgg ttgcgggggc cggatttgaa ccgacgacct tcgggttatg agcccgacga
    26701 gctaccaggc tgctccaccc cgcgcctgaa actcggcaaa ttctactcgt tttgggtaaa
    26761 agatgcaaat actgctggga tttggtgtac cgagacggga cgtaaaatct gcaggcatta
    26821 tagtgatcca tgccacattt tgtcaacgtt tattgctaat cacgtgaatg aatatccagt
    26881 tcactttcat ttgttgaata cttttgcctt ctcctgctct cccttaagcg cattatttta
    26941 caaaaaacac actaaactct tcctgtctcc gataaaagat gattaaatga aaactcattt
    27001 attttgcata aaaattcagt gagagcggaa atccaggctc atcatcagtt aattaagcag
    27061 ggtgttattt tatgacgacg attctcaagc atctcccggt aggtcaacgt attggtatcg
    27121 ctttttctgg cggtctggac accagtgccg cactgctgtg gatgcgacaa aagggagcgg
    27181 ttccttatgc atatactgca aacctaggcc agccagacga agaggattat gatgcgatcc
    27241 ctcgtcgtgc catggaatac ggcgcggaga acgcacgtct gatcgactgc cgcaaacaac
    27301 tggtggccga aggtattgcc gctattcagt gtggcgcatt tcataacacc accggcggcc
    27361 tgacctattt caacacgacg ccgctgggcc gcgccgtgac tggtaccatg ctggttgctg
    27421 cgatgaaaga agatggcgtg aatatctggg gtgacggtag cacctataaa ggaaacgata
    27481 tcgaacgttt ctatcgttat ggtctgctga ccaatgctga actgcagatt tacaaaccgt
    27541 ggcttgatac tgactttatt gatgaactgg gcggccgtca tgagatgtct gaatttatga
    27601 ttgcctgcgg tttcgactac aaaatgtctg tcgaaaaagc ctactccaca gactccaaca
    27661 tgcttggtgc aacgcatgaa gcgaaggatc tggaatacct caactccagc gtcaaaatcg
    27721 tcaacccgat tatgggcgtg aaattctggg atgagagcgt gaagatcccg gcagaagaag
    27781 tcacagtacg ctttgagcaa ggtcatccgg tggcgctgaa cggtaaaacc tttagcaacg
    27841 acgtagaaat gatgctggaa gctaaccgca tcggcggtcg tcacggcctg ggcatgagcg
    27901 accagattga gaaccgtatc atcgaagcga aaagccgtgg tatttacgaa gctccgggga
    27961 tggcactgct gcacattgcg tatgaacgtc tgttgaccgg tattcacaac gaagacacca
    28021 ttgagcagta tcacgcgcat ggtcgtcagt tgggccgtct actgtaccag gggcgttggt
    28081 ttgactccca ggcgctgatg ctgcgtgact ctctgcaacg ctgggttgcc agccagatca
    28141 ctggtgaagt taccctggag ctgcgccgtg ggaacgatta ttcaatcctg aataccgtct
    28201 cagagaacct gacctacaag ccagagcgtc tgacgatgga aaaaggcgac tcggtgttct
    28261 cgccagatga tcgtattggt caattgacca tgcgtaatct ggatatcact gatacccgag
    28321 agaaactttt tggttatgcc aaaactggcc tgctctcctc ctctgccact tcaggcgtgc
    28381 cgcaggtgga gaatatggaa aacaaaggcc agtaattcga catcaaccct gcccttcagg
    28441 ggggggttac cttgcttatc ctctgtaaac tcctttctga acatcacata atcgaaaaca
    28501 tcatggtttg catccacaac ccgtgagtct cctttatagt gtgcaatcac ttcctcaagc
    28561 gtttgcggct gttccggctt tgttaccccc atccacgcat taataaggta attattgtaa
    28621 gctgtcgaaa agatgttgtt ttccgttgtg cgatccacgc catccccaag aacggggcta
    28681 taccagataa acatcgggac atgatatgcc tgctggctag cctccctgcc tccatgaaaa
    28741 tagacgttct tcttcgtagg gtcacgttcc aggccgtggt cggcaaaata catgaccgag
    28801 gcgcggcgat cttttaataa ttcaaaaacc tgacctagca aactatctgt gtaatgaatg
    28861 gagttgtcat agcaggcatc ctgatcgtcc tgcggttgaa acacggcgct ggattgcgga
    28921 taggcgctac aagccggttc atggcttcca tttaaatgaa gaacaatcag tttcttttgc
    28981 tgcgtatttt gctgtaatgc ttgcgataaa tgcggcaaca acaattcatc aaatcctctg
    29041 acataaactg tttccatggc gcgcatggcg atactggtaa ctgcagtacc attctgccga
    29101 aaagcggatt gcgagcttag ccagaaagtc tgaaatcctg cttgattagc catattaata
    29161 atgttgtcgg ggtaattatg aatgtcatga ctcaaaacag aatcagcagt taaagaaagg
    29221 ggaaccgaca gcgcagtgta aggtgcgctg cttattgctt gattaaacag tttgatctgt
    29281 tttctttgtg cttcaacttg cggtgtcgta gagcgtgtat atccatacaa agacatattg
    29341 tcgacacgta cagactcccc cacaatcaac acgtaggtat caatacctgt atccctgact
    29401 gataattgaa aatacggcac cgtgtttgca attgagagta atctttgatg ctctttcgct
    29461 gctaaagcaa aatagttgag attgaaaaac ggcgtatagg tagcaaatcg cgacgctagt
    29521 atatatggac tgaacgtttt tttatttttt gcatctttat aagcaaattg acaagcggaa
    29581 aataaactgc ccgaaatgac aatcagcaat aatattcctg tcactttttt tgtcggcaag
    29641 gaaacatcat attttattat tactgcccaa aaaagaaggg ataaaaaagc aaaggcacat
    29701 agataaggga tatacatccc cagcattttg acaacttcat ccggatcact ctggagcaca
    29761 ctaatcgcaa atccatcatt gaaagttgta ccaaaggtac accatgaata gaggctaata
    29821 ctcatgtctg ccgcagtcag taacacaaat ggtgcggcaa caagcaggcg aagcggaagg
    29881 gatgatatac gttttacaac caggatggtc aggacgaaga acagcaatcg ctgaaccata
    29941 tgcgcgccgg aagaggcgac taagttcgta ataccaaaaa caataacaca aagatacaac
    30001 aaccaatgag atttaaaact tctagcaaat ttgttgaata ctgtcatcca gaaatccaag
    30061 ttcacaacca atataaacaa ttatcaacaa tgttcaattt tatatcagac atgtttttca
    30121 actctgatcc atatcagacc gaaacactgt tagttttttc tttttaaatg atgcggccat
    30181 tgcgttctca gtaattaaat aacattatca atgcgtctct aaacattacg cagaacgtag
    30241 ccatcagtag ttatactgac aggagcagga tggtttatcg agaagtaaac atatccgtat
    30301 tcgtaccgga actggagttg acacaataaa gtgccaatta tgtcagcaga agggaaaagt
    30361 ttataactaa ggcgtaccgg caccatcgtt tcaaggtacc agctacgagt aaagcaactg
    30421 gacgagatac agatacctga caaccattcc tcagaccagg accaaaacga aaaaagacgc
    30481 ttttcagcgt ctcttttctg gagtttggta ccgaggacgg gacttgaacc cgtaagccct
    30541 attgggcact accacctcaa ggtagcgtgt ctaccaattc caccacctcg gcacggatac
    30601 tactttttag ttcgggatat cgctggtcgg cttagccgga gcagctggtt gagtttgttc
    30661 ggttttcgcc ggtgcactca gattttccca ttcgctacct ttattggttt tgttgctgtt
    30721 gatgttaccc agcaccagac tgatgatgaa gaataacgtt gccagcagcg ccgtcatgcg
    30781 ggtcatgaag ttaccagaac cacttgaacc aaacagcgta gcggaagcgc ctgctccgaa
    30841 ggaggctccc atatcagcgc ctttaccttg ctgcagcatg atcagaccaa caaggccaat
    30901 tgccacaata aggaaaacta ccaaaagagc ttcgtacata atcaacctgt tccttgcgga
    30961 gttgccgcgt accaatgctt caaccaataa agcgggagtt tttaatcttt cccactgaag
    31021 cgggtgtgaa tactaaccaa agcgaatgac cttcgcaagg gcaattttat cgcattgtat
    31081 caactgcgga aaaaaacagc aaaaccgatg tgttcgctga gaaaaaaggc gacaatcgcc
    31141 gcctttttag ccagtcattt atcgttttaa acggctttta ctgcatcggc gatgcggtgt
    31201 gcaaattcag tcacctgcgc ttcgtcttcg ccttccacca tcacgcgaat taacggttcg
    31261 gtgccggatt tacgcagcaa cacgcgtcca cggttgccca gcgcagcttc aacctctgcg
    31321 gtcacggctt taactgactc atgctcaagc ggatcgccgc tacctgcggt gtaacgcacg
    31381 ttaaccagaa tctgcgggaa cattttcatg ccgctgcaaa ggtcgtgcag gctcatatgg
    31441 ttacgtgcca tcgccgccag cacctgcaag ccagcaacga tgccgtcacc ggtagtggtt
    31501 ttatccagca ggatcacatg accggaattc tctgcaccga tacgccagcc tttctcctgc
    31561 attttttcca gtacgtagcg gtcacccact ttcgcgcgcg caaatggaat acctaactgt
    31621 ttcagcgcca gctcaagccc catgttactc atcaatgtac ccacagcacc accacgcagc
    31681 tggccctgac gaagaccttc acgcgcgatg atatacatga tctgatcacc atcgacttta
    31741 ttgccttcat ggtcaaccat aatcacgcga tcgccgtcgc cgtcgaaggc aataccgaga
    31801 tccgcttttt cagccagcac acgagcctgg agcgcgcgaa cgtcggtagc ccccacttcg
    31861 gcattgatgt ttacaccgtt cggctcacaa ccgatagcga taacgttcgc ccccagttca
    31921 cgcagcacgt tcggcgcgat gtgataagtc gcaccgtttg cgcaatccac cacaatcttc
    31981 agttcgctga ggctaagttc gttcgggaac gtggctttgc aaaactcgat atagcgaccc
    32041 gcagcatcaa cgatacggct ggctttaccc agttctgccg aatcaacaca gctgatctct
    32101 ttttccattt ccgcttcgat ggcctcttct accgcatccg gtagtttggt gccgtcgata
    32161 gagaaaaatt taatgccatt atcgtagaac gggttatgcg atgcagatat cacaattccg
    32221 gcctctgcgc ggaaggtacg cgtcagataa gccacggccg gtgttggcat cgggccagtg
    32281 aagagtgcgg aaaggcccgc tgccgccaga cccgcttcca gtgctgactc cagcatatag
    32341 ccagaaatac gcgtgtcttt actaataatg atcttacggg agccgtggcg cgccagcact
    32401 ttacccgcgg cccaacccag cttaagcaca aaatcaggtg tgatcggcgc atcccctaca
    32461 cgaccacgaa tcccatcggt accgaaatat ttacgattac tcatagcgtt tgttttcctt
    32521 tgcagacaga gtggcttcca ccacccgcat cgcttctacg gtttctttga cgtcatgaac
    32581 acgaataatg tgcgcgcctt gcattgcggc aatgaccgca caggccagac taccgctcag
    32641 gcgctcggac ggccccacgt tcagcagctg cccaatcatc gattttcgtg acatacctac
    32701 caacagcggc aggttgaaat ggtgaaattc agccaggcgc gccagtaatg aatagttatg
    32761 ggagagattt ttaccgaaac cgaatccggg gtcgagcaac aatttctctt ttgcgatacc
    32821 cgcctgctcg caacgtgcta tttgctcaat aaagtagcga ttcacttctg caaagacatc
    32881 gtcatacttc ggagcttcct gcatggtttt tggatttccc tgcatatgca tcagacaaac
    32941 cggtaaaccg gtttctgccg ccgcctccag agcgccaggt tcggaaaggg agcggatatc
    33001 gttaataatg tgagcgccaa ctttcgctga ctcacggatg acttctggtt tggatgtatc
    33061 taccgagagc cagacttcga agcgttgagc aattgcctca accacaggaa taacacgttg
    33121 caactcttct tcaacgctaa cttccgccgc ccctgggcgc gtggactcgc caccaacgtc
    33181 aatgatcgtc gcgccagcgt tgatcatcag attcgcatgt ttcaccgcat ctatcagcga
    33241 gttatgcgtg ccaccatccg aaaaggaatc aggcgtgacg ttgaggatcc ccattacgtg
    33301 aggatggcta aggtccagtg aagtgccctg ggcaaagagt ttcatggtgt tatccctggt
    33361 atgaattgat aagaaaaaac cccggagcac gccccggggt tttcggtaca aatacagtca
    33421 tctgatgcgg gaacttactt gtcgcctaac tgctctgaca tggtgttacc cgggttcggc
    33481 gtacgcggtt catcaaccgg acgaggagcc tttggactac cattgtcgcc agaattgtta
    33541 gaagcgcctg gttcttccca gcccgctggc ggacgtacat cgcgacgtgc catcaggtca
    33601 tcaatctgcg gtgcgtcgat agtctcatat ttcatgagag catctttcat cgcatgcaga
    33661 atatccatat tgtcggtcag aagctgacgc gcacgattat agttacgctc aatcagtgct
    33721 ttcacttcct ggtcgatgat acgtgcagtt tcatcggaca tatgtttcgc tttcgctacg
    33781 ctacggccga ggaacacttc accttcttct tccgcgtaca gcagtggacc caatttctca
    33841 gagaagcccc actgagtcac catattacgc gccaggttgg tcgcaacttt aatatcgttg
    33901 gacgcaccgg tagaaacatg ttccggcccg tagatgatct cttctgccag acgaccaccg
    33961 tacagcgtag aaatctggct ttccagtttc tgacggctgg cgctgattgc gtcgccctca
    34021 ggcaagaaga aagtcacacc cagcgcacga ccgcgtggga taatcgtcac tttgtgcacc
    34081 ggatcgtgtt ccggcaccaa gcgaccgata atcgcatgac ccgcttcgtg gtaagccgtc
    34141 gattctttct gcgcttccgt catcaccatg gagcgacgtt ccgcacccat catgatttta
    34201 tctttcgctt tttcgaactc aatcatcgac acaacgcgtt tgttgccacg agcagcgaac
    34261 agtgccgctt cgtttaccag gttcgccagg tcagcaccgg agaaaccagg agtaccacgg
    34321 gcaatgattg ccgcgtcgat atcgggtgcc aatggtacgc gacgcatgtg aactttcagg
    34381 atctgctcac gaccgcgaac atctggcaag ccaaccacaa cctgacggtc gaaacggcca
    34441 ggacgcagca gggctgggtc aagaacgtcc ggacggttag tcgcggcgat aacgatgata
    34501 ccttcgttac cttcgaagcc atccatctca accagcatct ggttcagagt ctgttcacgt
    34561 tcatcgtgac caccgcccag accagcgcca cgctggcggc ctacggcgtc gatttcatcg
    34621 ataaagatga tgcacggtgc cgctttcttc gcctgttcga acatgtcacg aacacgggat
    34681 gcacccacac cgacgaacat ttctacgaag tcagaaccgg agatagtaaa gaacggaact
    34741 ttcgcttcgc ctgcaatcgc tttcgccagc aacgttttac cggtacccgg aggaccgacc
    34801 atcaggacgc ctttcgggat cttaccgccg agtttctgga agcggctcgg ctcgcgcaga
    34861 tactctacca gttcagcaac ttcttctttt gcttcgtcgc agcccgcaac gtcagcaaag
    34921 gtcgttttga tctgatcttc cgtcagcatg cgcgctttgc tcttaccaaa cgacatggca
    34981 cctttgccac cgccgccctg catttgacgc atgaagaaga tccagacacc aatcagcaac
    35041 agcatcggga accaagagat gaagatagaa gccagcaggc ttggttcttc aggcggttca
    35101 ccgacaacct tgacgttctt ggtcaacagg ttatccagta atttcggatc ctgaaccgga
    35161 atgtaagtgg tataacggtt actatctttc ttggtaacgt tgatttcacg tccgttgata
    35221 cgcgcttcac gaacctggtc gttattgacc tcttgtagga aggtagagta atccacctta
    35281 cggccattag actcgctggg cccaaagctc tggaatactg acatcagcac aacggcaatg
    35341 accagccaga gtattaggtt tttcgccatg tcactcaagg gattaacctc ttattacaac
    35401 tgtgttaaaa acagcgtcag gatactctat atccagcatc tttcaaactt tcgtctgaaa
    35461 tctcccggtt agggtttacg cccggtcgct acaatataca cttcccgcga acgtgcacga
    35521 gaagagtccg gcttacgaac tttgaccttc gtaaacaggg agcgaatttc ccttagatac
    35581 tcatcgaaac cttcgccctg gaacaccttc actacaaaac tgccacctgg cgctaataca
    35641 tcacgacaca tttctaacgc cagttccacc agatacatgg cacgggggat atccaccgcc
    35701 ggtgttccgc tcatgtttgg tgccatatcg gacatgacaa cctggacttt gctgtcgcca
    35761 acgcgctcca gcagtgcttt catcaccagt tcatcacgaa aatcgccctg aagaaagtcc
    35821 acaccaacga taggatccat aggtaaaaga tcgcaagcga tgatgcggcc tttgccgcca
    35881 atttgggtga ccacatattg tgaccaacca cccggagcag caccaaggtc gacaaccgtc
    35941 attcccggtt taaagagttt gtcactttgc tgtatttcat caagtttaaa ccaggcacgg
    36001 gaacgtaacc cctttttctg tgcctgttga acatatttat cgctaaagtg ttcctgaagc
    36061 cagcggctgg agctggcaga acgcttctta cctgtcattt aactttccca tggggataac
    36121 tcatcgtaac caattgcgta aatttttacg cgcctatttg gtgatatatg ggagatggcg
    36181 gtagaatgac ccgttttcaa tcccaacgta agcaaaaaat acgatgaatc tgagtactaa
    36241 acaaaaacag cacctgaaag gtctggcaca tccgctcaag ccagttgttc tgcttggcag
    36301 taatggtttg accgaagggg tgctggccga gattgaacaa gcgttagagc accatgaact
    36361 catcaaggtg aaaatcgcca ccgaagatcg cgaaacaaaa accttgatcg tggaagctat
    36421 cgtgcgcgaa accggcgcct gtaatgtaca ggtcatcggt aaaacgctgg tgctttatcg
    36481 cccaactaaa gaacgtaaaa tctcgctgcc acgctaagat tatcctaaag ttacacacat
    36541 ttgctgtgta aaacgagggg ttttccgcag gcaggagagc aaaatgccac gctctgttcg
    36601 ttgataaaag gccgcatagc ggcctttttc ctttctttac aatacatcaa catcttgatg
    36661 tattgggtaa ttcttacagg tattccacct taattacttc aaattctact tcgccgcccg
    36721 gcgttttgat gaccacaaca tcatcttctt ctttgccgat caggccacga gcaataggcg
    36781 agtttacaga aatcaggttt tgtttaaagt cagcttcgtc atcgccaacg atgcgataag
    36841 tctgttcttc gtcagaatcc agattcagca ccgttacggt agcaccaaaa ataacgcgcc
    36901 cattgttggg cattttggtg acatcaatca cctgcgcgtt cgacagcttg gcttcgatgt
    36961 ctttaatacg gccttcgcag aaaccctgct gttcacgagc tgcgtggtat tcggcgtttt
    37021 ctttcaggtc gccatgctca cgcgcttccg cgatagcagc aatgatttca ggacggcgca
    37081 cagatttcag aaaatccagc tcttcgcgta atttttcagc gccgcgtaag gtcatcggaa
    37141 tagcttgcat ttgttatacc tcttgaatat tcctgatggg gcaagtcttc acccaccccg
    37201 gctgttcggc ctgcccggca taactcctca ccgggaccag aagcaaaaaa ataccgaccc
    37261 gggtacaagt cccaggtcag ctacaattca cattttgata gtcattttac cctgaagttc
    37321 ccgaagggtc atcgtttact ttatagggcg ttgcgccgta gtatgacggc tcgattccag
    37381 gttgttagcg cgagattatg cgattttcca gatttatcat cggattgacc agctgtatag
    37441 cgttcagtgt tcaggccgcg aatgttgatg agtacgttac tcaactcccc gctggtgcca
    37501 accttgccct gatggtgcaa aaagtcggcg cgtcggcccc cgctattgat taccacagtc
    37561 agcagatggc gctgcccgcc agtacgcaga aagtgattac tgcgctggcg gcgttgattc
    37621 aactcggccc cgattttcgt ttcaccacga cgcttgaaac caaaggcaat gtggaaaacg
    37681 gcgtacttaa gggtgactta gtggcgcgat ttggtgccga tccgacgtta aaacgtcagg
    37741 atattcgcaa tatgatcgcg actttgaaaa aatctggcgt caaccaaatc gatggcaatg
    37801 tgttgataga tacctccatt ttcgccagcc acgataaagc ccccggctgg ccatggaatg
    37861 acatgacaca atgctttagc gctccgcctg ccgctgccat agttgaccgc aactgtttct
    37921 ccgtctcgct ctacagtgcc ccaaaacctg gtgatatggc ttttatacgc gtggcatctt
    37981 attaccccgt tacgatgttc agccaggtac gcaccctccc ccgaggttct gccgaagcgc
    38041 aatactgcga actggatgtc ataccaggcg acctgaaccg ctttacgctg acgggatgcc
    38101 tgccacaacg ttctgagccg ctcccgttgg cttttgccgt gcaggatgga gccagctatg
    38161 ccggggcaat tctgaaagat gagttaaaac aggcaggtat cacctggagc ggaacactgc
    38221 tgcgccagac tcaggttaac gaacctggaa cggtagttgc cagtaaacag tcggccccgt
    38281 tgcacgatct gcttaagatt atgctgaaaa agtcggacaa catgatcgcc gatacggttt
    38341 tccgcatgat aggccatgcg cgcttcaatg tgcctggaac atggcgggcc gggtcggacg
    38401 ccgtgcgtca gatcctgcgc cagcaagccg gtgtcgatat tggaaacacc attattgccg
    38461 atggttcagg gctttcgcgg cataacctga ttgcccccgc caccatgatg caggtgttgc
    38521 aatacattgc ccaacacgac aatgaactta actttatctc catgctgccg ctggcgggct
    38581 atgatggctc tttgcagtac cgtgcaggtc tgcatcaggc gggcgtggat ggaaaagtct
    38641 cagcgaaaac cggttcgttg cagggggtat ataacctggc gggattcatt accacagcga
    38701 gcgggcaacg aatggcgttt gtacaatatc tttctggcta tgcagtagaa ccggcggatc
    38761 agcgtaatcg ccgtattccg ttagtgcgtt ttgaaagccg tttgtataaa gatatttatc
    38821 agaacaatta gtcaaaaaga aaccccggca cat
//
LOCUS       DADLFA010000014        37820 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000014 DADLFA010000000
VERSION     DADLFA010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37820
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.014"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..378
                     /locus_tag="JQH65_000639"
     CDS             <1..378
                     /locus_tag="JQH65_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY6138143.1"
                     /translation="ICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEKF
                     MNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSHRDFLNLKEIKLEHPLPSIKLYI
                     SYNKSSLNNLVFSRFIDRLNESF"
     gene            525..2042
                     /locus_tag="JQH65_000640"
     CDS             525..2042
                     /locus_tag="JQH65_000640"
                     /inference="COORDINATES: protein motif:HMM:NF014273.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HAY6138144.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEDDRIKAINEIRMAIHQ
                     VSPFREEPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGFTQPIVVTHTDK
                     NAMEIVDGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQIT
                     AMSEIVRELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(2043..3192)
                     /gene="ybdL"
                     /locus_tag="JQH65_000641"
                     /pseudo
     CDS             complement(2043..3192)
                     /gene="ybdL"
                     /locus_tag="JQH65_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
     gene            3301..4389
                     /gene="hcxA"
                     /locus_tag="JQH65_000642"
     CDS             3301..4389
                     /gene="hcxA"
                     /locus_tag="JQH65_000642"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HAY6138145.1"
                     /translation="MPHNPIRVVVGPANYFSHPRSFNHLNDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4399..4596)
                     /locus_tag="JQH65_000643"
     CDS             complement(4399..4596)
                     /locus_tag="JQH65_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HAY6138146.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4778..6883)
                     /gene="cstA"
                     /locus_tag="JQH65_000644"
     CDS             complement(4778..6883)
                     /gene="cstA"
                     /locus_tag="JQH65_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HAY6138147.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPKRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(7064..7477)
                     /gene="entH"
                     /locus_tag="JQH65_000645"
     CDS             complement(7064..7477)
                     /gene="entH"
                     /locus_tag="JQH65_000645"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HAY6138148.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7480..8226)
                     /gene="dhbA"
                     /locus_tag="JQH65_000646"
     CDS             complement(7480..8226)
                     /gene="dhbA"
                     /locus_tag="JQH65_000646"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase"
                     /protein_id="HAY6138149.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8226..9083)
                     /gene="entB"
                     /locus_tag="JQH65_000647"
     CDS             complement(8226..9083)
                     /gene="entB"
                     /locus_tag="JQH65_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HAY6138150.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFELQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(9097..10707)
                     /gene="entE"
                     /locus_tag="JQH65_000648"
     CDS             complement(9097..10707)
                     /gene="entE"
                     /locus_tag="JQH65_000648"
                     /EC_number="2.7.7.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase"
                     /protein_id="HAY6138151.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GELQLSYRELNQAADNLACTLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
                     HNLQDAINHPADGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLSQWLASRASA"
     gene            complement(10717..11892)
                     /gene="entC"
                     /locus_tag="JQH65_000649"
     CDS             complement(10717..11892)
                     /gene="entC"
                     /locus_tag="JQH65_000649"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HAY6138152.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKVQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVLASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12267..13223
                     /gene="fepB"
                     /locus_tag="JQH65_000650"
     CDS             12267..13223
                     /gene="fepB"
                     /locus_tag="JQH65_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HAY6138153.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPTGLNASQSQGKRHDIIQLSGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(13227..14477)
                     /gene="entS"
                     /locus_tag="JQH65_000651"
     CDS             complement(13227..14477)
                     /gene="entS"
                     /locus_tag="JQH65_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HAY6138154.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWLVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAEIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14588..15592
                     /gene="fepD"
                     /locus_tag="JQH65_000652"
     CDS             14588..15592
                     /gene="fepD"
                     /locus_tag="JQH65_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HAY6138155.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15589..16581
                     /gene="fepG"
                     /locus_tag="JQH65_000653"
     CDS             15589..16581
                     /gene="fepG"
                     /locus_tag="JQH65_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HAY6138156.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16578..17393
                     /gene="fepC"
                     /locus_tag="JQH65_000654"
     CDS             16578..17393
                     /gene="fepC"
                     /locus_tag="JQH65_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HAY6138157.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIKHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTHWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLERTAPSTANS"
     gene            complement(17390..18523)
                     /gene="fepE"
                     /locus_tag="JQH65_000655"
     CDS             complement(17390..18523)
                     /gene="fepE"
                     /locus_tag="JQH65_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HAY6138158.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSSNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(18738..22619)
                     /gene="entF"
                     /locus_tag="JQH65_000656"
     CDS             complement(18738..22619)
                     /gene="entF"
                     /locus_tag="JQH65_000656"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HAY6138159.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVDGLAQADTLRMRFTEDNGEVWQWVDDALTFELPEIIDLRTNIDPHGTAQALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYQFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTNLCYNAPLKPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYLLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTSV
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFVPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPLLPLSANGKLDRKALPLPELKAQAPGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQVARQVTPGQVM
                     VASTVAKLATIIDAEEDSTRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYI
                     DPQWSIIGIQSPRPNGPMHTAANLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
     gene            complement(22616..22834)
                     /locus_tag="JQH65_000657"
     CDS             complement(22616..22834)
                     /locus_tag="JQH65_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbtH family protein"
                     /protein_id="HAY6138160.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            complement(22837..24039)
                     /gene="fes"
                     /locus_tag="JQH65_000658"
     CDS             complement(22837..24039)
                     /gene="fes"
                     /locus_tag="JQH65_000658"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HAY6138161.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     PIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDATAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            24282..26522
                     /gene="fepA"
                     /locus_tag="JQH65_000659"
     CDS             24282..26522
                     /gene="fepA"
                     /locus_tag="JQH65_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HAY6138162.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATRDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            26697..27317
                     /gene="entD"
                     /locus_tag="JQH65_000660"
     CDS             26697..27317
                     /gene="entD"
                     /locus_tag="JQH65_000660"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HAY6138163.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKAEHLAGRIAAIYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(27439..27591)
                     /locus_tag="JQH65_000661"
     CDS             complement(27439..27591)
                     /locus_tag="JQH65_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAY6138164.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            28044..29162
                     /locus_tag="JQH65_000662"
     CDS             28044..29162
                     /locus_tag="JQH65_000662"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HAY6138165.1"
                     /translation="MPLPDFHVSEPFTLGIELEMLVVNPPGYDLSQDSSILIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQVTVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            29228..29476
                     /locus_tag="JQH65_000663"
     CDS             29228..29476
                     /locus_tag="JQH65_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HAY6138166.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            29541..29909
                     /locus_tag="JQH65_000664"
     CDS             29541..29909
                     /locus_tag="JQH65_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HAY6138167.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            30003..30656
                     /gene="nfsB"
                     /locus_tag="JQH65_000665"
     CDS             30003..30656
                     /gene="nfsB"
                     /locus_tag="JQH65_000665"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HAY6138168.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            30764..32011
                     /locus_tag="JQH65_000666"
     CDS             30764..32011
                     /locus_tag="JQH65_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAY6138169.1"
                     /translation="MQDLISQVGDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGT
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESILNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPAGNDIRSLAGAFKQ"
     gene            complement(32079..33455)
                     /gene="pheP"
                     /locus_tag="JQH65_000667"
     CDS             complement(32079..33455)
                     /gene="pheP"
                     /locus_tag="JQH65_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HAY6138170.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYICSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNEVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSHRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(33557..36700)
                     /gene="cusA"
                     /locus_tag="JQH65_000668"
     CDS             complement(33557..36700)
                     /gene="cusA"
                     /locus_tag="JQH65_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HAY6138171.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQVGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKNDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIVEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGGAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(36712..>37820)
                     /gene="cusB"
                     /locus_tag="JQH65_000669"
     CDS             complement(36712..>37820)
                     /gene="cusB"
                     /locus_tag="JQH65_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HAY6138172.1"
                     /translation="RKILFWYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVR
                     IDPTQTQNLGVKTATVTRGPLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVG
                     DKVQKGTPLLDLTIPDWVEAQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLI
                     ATQKIQTRFTLKAPIDGVITAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWL
                     VKDASQFTLTVPARPDKTLTIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWL
                     QLNTASEPMLLIPSQGLIDTGSEQRVITIDADGRFVPKRVAVFQASQGVTALRSGLAE
                     GEKVVSSGLFLIDSEANISGALERMRSESATHAH"
BASE COUNT         9071 a        10547 c         9636 g         8566 t
ORIGIN      
        1 atttgtagta ataagcatcc gcgaattact gacaatagca cttatgaaca aattatggca
       61 gaagagttca cccaattaat atccaaatca gcaggagttg atgatattca aatggaaata
      121 gacgagaagt ttatgaatcg caaaatttca tttcgtggtt cctctttgct gacaataatt
      181 aatagcattg cagttacaga tttactggga atagtcccct atgaacttta taactcccac
      241 cgtgactttc ttaatttaaa agagataaaa ctagaacatc cgttaccttc aataaagctt
      301 tacatttcct ataataaatc ttctttaaat aatttggtgt tctcccgatt tattgaccgt
      361 ctaaatgaga gtttttgata tataactaca gacagctact ataactttca tctatttatt
      421 cacagcgcga atgaaaaata aaacactctg ctttacagtg tttactttat aattaacagt
      481 cttatctcac tgttcaccct gtaacgttca gagagcgtct tcagatgtct atttataaaa
      541 ttcctcttcc gctcaatatt ctggaagctg cacgagagcg tattacctgg acactcaaca
      601 ccctaccccg cgtttgtgtc tcattttctg gcggcaaaga ctctggttta atgctgcatc
      661 tgacagccga acttgcccga caaatgggca aaaaaatctg cgttttgttt atcgactggg
      721 aggcgcaatt ctcttgcact attaactatg ttcagtccct gcgcgagttg tacaccgatg
      781 tcatcgaaga gttttactgg gttgcgctcc cgcttacgac gcaaaattcc ctttcacaat
      841 accaacccga atggcagtgc tgggaacctg atgtcgaatg ggtgcgtcag cccccgcaag
      901 atgcgataac cgatcctgac tttttctgct tttaccagcc aggcatgacc ttcgaacaat
      961 ttgtacgtga gtttgccgaa tggttttcac aaaaacgtcc ggcggcgatg atgatcggca
     1021 tccgtgcgga tgagtcctac aaccgttttg tcgccatcgc cagtttaaat aaacaacgtt
     1081 ttgccgacga taaaccatgg actacggccg caccaggcgg tcatagctgg tacatttacc
     1141 ccatttacga ctggaaagtg gctgatatct ggacctggta tgcaaatcat caaagtctct
     1201 gcaacccact gtataaccta atgtatcagg caggcgttcc tctgcgtcat atgcgaattt
     1261 gcgaaccttt tggcccggag caacgacaag gattatggct ctatcacgtt atcgaaccgg
     1321 atcgctgggc tgctatgtgc gcacgagtca gcggggtaaa aagtggcggc atttacgccg
     1381 gacatgacaa tcatttctat gggcatcgga aaatcctcaa gccagaacat ttagactggc
     1441 aagaatatgc attattgctg ctcaatagca tgccggaaga tgatcgcatc aaagcgatta
     1501 atgaaatccg catggcgatc catcaggtta gcccctttcg tgaagagcca gtggattgcg
     1561 ttctgtgggt taaaaacagc caacttatgc ctaatgatta caacccaaac aacgtggcac
     1621 cacccgaaaa aaaactgctg caaaaatcca ttgaaataga tggttttacc caacccattg
     1681 tggtcacaca tacggataaa aatgccatgg aaatcgtgga tggttttcac cgccatgaaa
     1741 ttggtaaagg ctcatcatca ttaaaattac gcttaaaagg ttatttgccg gtgacctgtc
     1801 tggaaggtac gcgtaatcaa cgcatcgccg cgactattcg tcataatcgc gctcgtggac
     1861 gccatcaaat caccgccatg tcggaaatcg tccgtgaact tagccagttg ggatgggacg
     1921 ataataaaat tggcaaagag ctgggaatgg acagcgacga agtgctgcgc ctgaagcaaa
     1981 ttaacggcct gcaagagttg tttgccgacc gtcaatattc ccgcgcctgg acggttaaat
     2041 agctaaagct ggcgcaggcg ttcagctgct gccagcaacg tcgattcctt cttggcaaaa
     2101 cagagacgaa tcagtttatg tgggaaggga tcggcgcaaa acaccgacag cggaatcgcc
     2161 gccacgccgt gctcccgcgt cagccactgg caaaactcaa catcatccag ggtagaaacc
     2221 tcgctgtaat ccaccagcaa aaagtatgta ccttcacacg gtaaaatctc cagccggctt
     2281 tcatttaaag cattcaccag aatatcgcgc ttctggcgat aaaagtccgg taacgcaaga
     2341 taatgctcag gttctgcacg tagcatatca gcaagcgcca gctgtgccgg ggtattcacc
     2401 gaaaaggtca gatactgatg taccttgcga atttcggcgc tgatgggcgc tggcgcaaca
     2461 caataaccca ctttccagcc ggtcatatga tagatcttgc caaatgaaga aaccgccact
     2521 gcccgctcac gcagctgcgg atgcgccagc acactggcat ggccctgttg tgaaaagttg
     2581 atgtgctcgt agacttcatc gctaatgaca aaaatctcgt gcccggcgat cgcctgccac
     2641 aaagcggcga aatcagcctg ctgccagaca gttgcactgg ggttatgcgg agtgttgagg
     2701 atcaccagtc tggtgcgctc gcttaataat gcggcaaatt cctgccagtc aaggtggttg
     2761 cagtgccata cgcttcacta ttcccccaga aagcgcgatg gcgggggcgt aactgtcata
     2821 gctgggatca aaacaaatca cttcatcgcc attgcgcacc agtgcggtaa tcgccgcgta
     2881 taacgcttcc gtcgcccctg ccgttacggt gatatcgcta tcggcatctg gttgatagcc
     2941 atacaaacgt tccgttttct gagcaatcgc ctcgcgcaag gcctgcacgc cggtcatggg
     3001 cgcgtattgg tttgccccct gggcaacgtg gtacgccagc cgctcctgta aatagcgcgg
     3061 accatcaaaa tcaggaaagc cttgcgacag gttaatcgcc tggtgttgct gcgccagcgc
     3121 gctcatctgg gtgaaaatag tggtgccaag ttgtggaagt ttgctttgtg gaatcagagg
     3181 gttatttgtc atttttattg tacctgacga agttgcgtgt tgaaggcact ataacacgat
     3241 gctagtattt ggcaatcaag acgtttagat gtctaaataa aacaataagg acaacacaac
     3301 atgcctcaca atcctatccg cgtggtcgtc ggcccggcta actacttttc acatccaaga
     3361 agtttcaatc acctgaacga ttttttcact gatgaacaac tttctcgcgc ggtgtggatc
     3421 tacggcaaac gcgccattgc tgcggcgcaa accaaacttc cgccagcgtt tggacttcca
     3481 ggggcaaagc atattttgtt tcgtggtcat tgcagcgaaa gcgatgtaca gcaactggcg
     3541 gctgagtccg gtgacgatcg cagcgtggtg attggcgtcg gcggcggcgc actgctcgac
     3601 accgcgaaag ccctcgcccg ccgtctcggt ctgccatttg ttgccgttcc gacgatcgcc
     3661 gctacctgcg ccgcctggac accgctctcc gtctggtata acgatgccgg acaggcgctg
     3721 cattatgaga ttttcgacga cgccaatttt atggtgctgg tggaaccgga gattatcctc
     3781 aatgcaccgc aacaatatct gctggcgggg atcggtgaca cgctggcgaa atggtatgaa
     3841 gcggtggtgc tggctccgca accagaaacg ttgccgctaa ccgtgcggct ggggatcaat
     3901 aatgcgcagg ccattcgcga cgtcttgtta aacagtagcg aacaggcact tgccgatcag
     3961 caaaatcagc agttaacgca atcattttgc gatgtggtgg atgctattat tgctggtggt
     4021 gggatggtcg gtggtctggg cgatcgtttt acgcgtgtgg cggcggctca tgccgtgcat
     4081 aacggtctga ctgtcctgcc gcaaaccgag aagtttcttc acggcaccaa agtcgcttac
     4141 ggaattctgg tgcaaagcgc cttgctgggt caggatgatg tgctggcgca attaaccggg
     4201 gcgtatcagc gttttcatct gccgactacg ctggcggagc tggaagtgga tatcaataat
     4261 caggcggaga tcgacaaagt gattgcccac accttgcgcc cggtggagtc cattcattac
     4321 ctgccagtca cgctgacacc agatacgttg cgtgcagcgt tcgaaaaagt ggaatcgttt
     4381 aaagcctgac gtgcaaattt agcagcagcg cgcgccgcct tttccaccgt agcgcgcgtc
     4441 ctgccgctca cggaaaaact cctcgtaggt catcggcgtt tgatcgggat ggttaacccg
     4501 catatgttcg acatagttgt cgtaatcagg cataccaatc atcagcttcg ccgcctgacc
     4561 taaatatttt ccggcttttg ccagtgaatc aaacatcttg gcctccgcta accatgttgc
     4621 tgaaagagtg ccggatgcgg cgtgaacgct ttatccggcc tacaaatgct atcaacgcac
     4681 tcggtcagtc ccctcgcccc atcggggaga gggttagggt gagggggaac cctcggcact
     4741 atgccaacat tcgccaacat cccccctcac tctgacttta gtgcgcgcct ttcgcctgcg
     4801 ctacgatctc ctcgacattt tccggcatcg gctcatacgg cgtttctttc gccgttggct
     4861 tcggatcttt caatgccgca agtgccgtct taatcgagaa cagtgccaga accaccacga
     4921 ccaccataaa gaagatggtt aacccggcat ccagacggtt gttgaacacc agttgcgcca
     4981 gctgtgactc agtatactgc gacggaatat tgccgctgtc gatcattgcc tggaacttgt
     5041 tagcaatggc caggaagccg actttcgcat ccgggctaaa cgctttctgc cagccagcgg
     5101 tcagggtaca aatcagcagc caggccgttg gtaccagcgc cacccaggcg taacgttgac
     5161 gtttcatctt gaacaacacc acggcacaga gcatcagcgc catccctgcc agcatctggt
     5221 tggcaatacc aaacagcggc cacagagtgt taataccgcc cagcggatcg accacgccct
     5281 gatggaggaa gtagccccac gccagtacgc acagcgccgt tgccagcagg ttagcaggca
     5341 gtgaatcggt ccgcttcagg ccaggagaca ccacgcccag cagatcctgc aacataaagc
     5401 gcgcagcacg cgtacccgca tctaccgccg tcagaataaa cagagcttca aacagaatgg
     5461 cgaagtgata ccagaacgcc acatccatca taccacccag cgcaccgtgc aggatgtaag
     5521 ccatccccac cgccaacgtc ggcgcaccgc ccgcacggga aatgatcgac tgttcaccca
     5581 cttcgctggc aatctggttg agcgtatctg gtgtaatgct aaagccccag ctactcacca
     5641 cctgcgcggc agaagcgacc acatccgccg tccctgctgg agccagcacc gccattgggc
     5701 tgttcatggc aaagtaaacg cccggatcga tgatacaggc ggaaaccagc gccataatcg
     5761 ccacgaagga ttccattaac attccaccgt agccgataaa acaagcctgc ccttcgttcg
     5821 ccagcatctt cggcgtggtg ccggaagaga tcagcgcatg gaagccagac accgcgccac
     5881 aggcgatggt gataaacagg aacgggaaca ggttaccggt ccataccggg ccagtgccat
     5941 cgacaaattt ggtcagcgca ggcatggtca gcgtcgggcg cataatcaaa atgcctaccg
     6001 ccagaccgac gatcgtcccg attttcagga aggtagagag gtagtcacgc ggggccagca
     6061 gcaaccacac cggcagcacc gccgccacaa aaccgtaacc caccagcatc caggtcagct
     6121 gcacgccggt aaagtcaaag tacggtgccc aggtcggact ttctgccacc cagccgccag
     6181 agataatggc gaaaatcagg aataccagac cgatgaccga cacttcacca atacgccccg
     6241 gacgcagata gcgcaggtag atccccataa acagcgccag cggaatggtg aacgcaacgg
     6301 tatacgttcc ccacgggcta tgagtcaggg ctttcaccac gatcatcgcc agtactgcaa
     6361 ggataatgac catgatcata aagcaggcca ccagcgctat caccccggcg gttggcccca
     6421 tctcttcttt gaccagctca cccagcgatc gaccgtcacg gcgcgtagaa acaaacagca
     6481 ccatgaaatc ctgcaccgca ccggcgagaa ccaccccggc gagtagccag atcatccccg
     6541 gcaggtagcc catttgcgct gccagcaccg gcccaaccaa cggacctgct ccggcgatgg
     6601 ccgcaaaatg gtgaccgaac agcactttct tgtccgtcgg cacatagtcc agcccgtcgt
     6661 tatggcgcac cgctggcgtc atacgcttcg ggtcaaccgc cagcacattt ttggcgatat
     6721 acaacccata aaaacggtaa gcgatcagat agatacagac cgacgccacc acaatccaca
     6781 gcgcgttgat ctgttcccca cgatttaaag caatgtatcc cagagcaaat gctcccatta
     6841 cagagagcac tgtccagacg aggtatttcc ctgatttgtt catagttgtt atccgtgtgc
     6901 gtgtccatag agatgttaca ttttgtttct ataacatctt tgtacgattt aacaacacgg
     6961 taactatgta aatcggagtt gaaaccaata tttaacttaa caatgttaac tcgatcactc
     7021 cgttaaccga gttttcctgc aatctcattt aattctgtcg ggctcatccc aaaactgccg
     7081 tacccagccg acaagtgcag caacgccgcc cctgttcatc gaaaacgacg atttcccagc
     7141 tctgattttg ccgcccaaga tgcagcggct ggcagacgcc gcgtaccttg ccttcagaca
     7201 ccgggcgatg atgggtggcg ttaagttccg tgcccaccac acactgcccg tcacgggtca
     7261 tcataaatcc ggccatcgat cccagcgttt cagccagcgc cgccgacgcg ccgccatgca
     7321 gcaggccaaa cggctgatga gtacgggtat caaccggcat ttcggcttcc agcacatcat
     7381 ctcccagacg ggtatacaca attcccagat gcgccaccat tgtgttatcg ctggtggcgt
     7441 tcagttcgtc gagcgttaaa tggcgtttcc agatcatgct tatgccccca gcgttgagcc
     7501 gccatcgacc acaatatcct gtagggtgat atggctggcg aggtcagagg cgaggaacaa
     7561 aatcgtgttg gcgatctctt gtggacgggc gattttcccc agcggaatgc cgagtttaaa
     7621 ctgctcgcca aagccgcgaa tacgctgttc ttcggcgtca tcgctcaccc acagcgtgcg
     7681 ttgcatatcg gtgtcggtgg agccaggcga aaccacatta cagcgcacgc cgctacccgc
     7741 cagttccagc ccgacgctca acgccaggct tttcagcgcc gctttcgatg cgccataagc
     7801 actcatacca atacgcggcg tgtgtgcggc gtcggacgcc acagtgacaa tcgccccgcc
     7861 ccgctgacgg cgaaactggt tcatggtttg ctggaacagg ttaaacgcac cgccgacgtt
     7921 aaccgcaaaa gtctgctgcc agtcctcttt actgagctga tcggtcgcgc ccatgcgcaa
     7981 aattcccgcc gcattgatca gcacgtccag tcgctccgtt tcagctaaca gtcgctgaca
     8041 tacctgcgcc acctgcccgg cgtcggcaac atccattact tcggtcgcaa agggatattg
     8101 ctcctgagtg aacgcttgat caaaacctgt aactttcgct cccgcctcaa caaacgccag
     8161 cgccgtggcg tagccgatac ctttacctgc gccggttacc cagacatttt taccgctgaa
     8221 atccattatt taacctcgcg ggagagtagc ttccaccagg cgtcgatggt cgggtttttc
     8281 gccagcatga caaagtcgat atcaccatgc actttgcgcc agcgcgccgc cagcgccatc
     8341 atgcgcaccg aatccagacc gtagtcgatc aggttgtcgt catcgaacgg ttcatcggac
     8401 tcgtccagca acggcaggat aacctcacgc agcgccgctt tgctggcggg gacaggtgct
     8461 ggcagtaatt cttcagtcat caccacccgg ccagaccgcc cggccacata tttcagcgac
     8521 atcaaatgct cgtcacggct gaaatcggcc agcgcatccg ccaccataaa cggtttaata
     8581 tcgcgcataa atgcgtcggt tgcggtggtc atacagccaa tgtgggcata taccccggta
     8641 ataatcagct ggttacgtcc actctctttg agcatttgct ccagcggaga acgatgaaac
     8701 gcgctgtagc gccacttcac cagcaccgtg tcgtcggcat ctggcgtcag gcgatccacc
     8761 accttttgct gttccggcga acgggtcagg cccggccccc acatatcatt caacagcgcc
     8821 cgatcttcat cgctctgctc tttcggctgg gcggtgtaat aaactgggat attgtgctgt
     8881 ttgcagtagt cgcgcagcgc agcaatattc gcgatcacct gctccatcat cgggcagttc
     8941 tcgccccaga agctgacaaa atagtcctgc atatcatgga ttaacaacgc ggcacgttgc
     9001 agttcaaagg cccagtcaac tttattctgc ggaatatcgt gagactccgg cagtgcgtaa
     9061 gcctgtaatt ttggaatagc catcgtgttc tctccttcag gctgatgcgc gtgacgccag
     9121 ccactgactt aattgttttt tatcgacttt cccgaccgcc gtcagcggaa gtgaatctac
     9181 acactccacg cgatccggta atttaaattc ggcaataccc tgttcacgca ggaaacgacg
     9241 cacctgcacc gcgcgcagcg gctcttttac caccagataa gcacagcttt tttcgcccat
     9301 cagctcatct tccatgctca ccagtgcggc atagatcacc gccggatggc gcagcagcag
     9361 gttttcgatc tcttcggcag cgatcttctc gccgccacgg ttgatctgat ctttctcgcg
     9421 cccctgcacg gtgatgtaac cctctggatc aatagagatc agatcgccgg aacagtaaaa
     9481 accgttggca tcaaaggcgc tggcattgtg ctgcggactt ttgtaataac cgcggaaggt
     9541 gtacggcccg cgcgtcatca ggcgtccgac ttccccttgc ggcagtggat ttccttcggc
     9601 atcggcaacc cacacttcat cgtccggaca cattgggtaa ccctgggtat ggataatttt
     9661 ctccgcgcta tcatcaagac gggtgtagtt caccagccct tccgccatgc caaacacctg
     9721 ctgcaactgg cagccaatct cagcgggaat acgcgccgca agggtggcag aaagacgtgc
     9781 gccgccgacc tgtaacagtt tcagcgaggc aagctgcgcc cggctttcgc cttcagccag
     9841 cgcctgcaac cacaggctga ctgccggcgg caccagcgcg gtgacgttca cctgatgttt
     9901 ttcaatcaat gggaagcaaa gcgtagcgct gggatcggcc gccagaacaa ccgttcctcc
     9961 ggcaagaaag acgcccagcg atcctggcga actcatggcg tagttatgag ccgccgggat
    10021 cgcgcacagg tagcgcgtct gttgtgtgaa ctgacaaatc tcgacgctac gacgcacgct
    10081 gtagtagtag tcgttatgag tgcgcgggat cagcttcggt gtaccggtgg tgccgccgga
    10141 aagctggaaa taggccactt catcagcagg tgatggcgtg gcagtaaaac cgtctgccgg
    10201 atggttaatc gcatcctgca agttatgctc accgctgtcg ttgtgcagtt gcaccacgcg
    10261 aatggaggaa tgttctgtga cgaaagtatt gaggaaatca tccccgctaa acagcgcatg
    10321 ttggcgatcg gcaatcagca atgcgggttc aatctggctg gcataggcgt tcagttcact
    10381 acgctgatgg ctgaacaacg ccagcaccgg cgcaacgccc agtttcagca gcgcgaaaaa
    10441 ggtaatatac aattcagcga cgttacccag ttgtaccagc gcggtttcac caggtttaat
    10501 gccctgacgg cgtaaagtac acgcgaggtt atccgccgcc tgattcagct cccggtaact
    10561 caactgtagc tcgccgtcga taaccgcgat gctgtcactc gcagcgtggc gagtcagaat
    10621 gtcggtcagc ggcaaatcct gccagtagcc tttttcccga tagcgacggg caaactcttc
    10681 cggccagcgg gtgaatggaa tgctcatcct cgctccttaa tgcaatccaa aaacattcaa
    10741 catggtagaa agtttgacgc ctgtttcgcg ccactcaccc aacggtgacg acgcaagcac
    10801 aatccccgct ccggcaaaca gacgcacctg attttcccgc agcttcgcgc agcggatggt
    10861 caccacccat tcgccgttac cttcgctgtc acaccaaccc acaatgccgc caaacagttc
    10921 gcggtcgaac ggctccagtt cagcaataac ctgagtcgcg gcctgatgcg ggaaaccgct
    10981 cagcgcgggg gtcggatgca gcagacaggc cagagtcagt gcgttttctt gcgaattcgc
    11041 tttaccttca aagggagttg cgagatgcca cagcgtcggc gtggtaatca attgtggaga
    11101 ggagggaacg tgtaactcac tactgcgttc gcgcagtacc tctttcatcg cctgagtcac
    11161 cagttcatgt tcatggcgat ctttttctga cgccagcaga cgattacccg cttcgcgatc
    11221 gagcacttca tccggctgac gacgcgcgga accggctaac ggaatggagc taaaacgctc
    11281 gccgtcttta cgtagcagca gttccgggct ggcccccagc aggacgccac catcagccag
    11341 cgggacatgg aagttgtaac taaccgggtt ttgcgcaatc aaccgttcca gcaatacgcc
    11401 actatcaatg gcggcgtcag tggtgatatc aatcaaccgt gacaacacca ctttgtcaac
    11461 ctgcggcgtg gcggtaagtg cggcagcgcg ggcaaccatc tgttcaaacg tggtttgttc
    11521 cggaattgcc tggcgttcca ccacattcag cgactggctg cgggtgaaac ggcgggcgga
    11581 agcttgtttt tcctgacggg agaacgactg ccaggattca ggaatataca gcgacgaagg
    11641 ctgacgtgga tcgaagggaa tcgccccgac catcaccgga tttttgatgc cctgcacttt
    11701 ggcatcggca aacagcgcgg cgagtttttg ctggaaggga ctgtcgggcg aatccccgtt
    11761 cacagccggt tcatcgaagc gggcgaaaca tcctgacgtc gtaaaactgc ggtacggcga
    11821 cataaagaaa aagcgattgg gcgcaagtgt tgccatggtc tgctgtactt cctcagccag
    11881 tgacgtatcc atatcatcct ccacaaaatg ataaaggctt taataatgat tatcatttat
    11941 attttcggtc gctaacctaa aagcaaacgc gcactatgtc aactcttgag gtaacgcaat
    12001 ctgcaactca gggttgcatc tggttgtggt tacaatgaaa atgagaagca ttattgatgg
    12061 attcgcataa gcgcaatgtg atgtcctgcg ccgttcttcc ccctctccct tccagggaga
    12121 gggctggggt gagggtcaat attcgcacca gtgctggctg ctcccatcac cctaaccctc
    12181 tccccaaagg ggcgagggta cggatagtgc gctttgtcga atttgtcatt acgcccttaa
    12241 ccttattaat aacaggaagc tgatttgtga gactcgcccc gctctaccgc aacgcccttc
    12301 tattaacagg acttttgctt tccggaatag ccgcagttca ggccgccgac tggccgcgtc
    12361 agattactga cagccggggc actcataccc tggaaagcca gccgcagcgt attgtttcca
    12421 ccagcgtcac cctgaccggc tcactgctgg cgattgatgc tccggtgatt gccagcggcg
    12481 cgaccacgcc aaataaccgc gtcgcggatg accagggctt tttacgccag tggagcaagg
    12541 tggcgaaaga acgcaaacta caacggctct atatcggcga accgagcgcc gaagccgttg
    12601 ccgcgcaaat gccggatctg attttaatta gcgcaaccgg cggggattcg gcgctggcgc
    12661 tgtatgatca gctttccacc atcgccccga cattaatcat caattacgac gacaaaagct
    12721 ggcagtcgct gttaacgcag cttggcgaaa ttaccgggca tgagaaacaa gcggcagagc
    12781 ggattgcaca gtttgataag caactggcag cggcgaaaga gcaaatcaaa ttaccgccgc
    12841 agccggtcac tgctattgtc tataccgccg ctgcacacag tgccaatctc tggacgccgg
    12901 aatcagcaca agggcagatg ctggaacaac tcggctttac gctggcgaaa ttgcccacag
    12961 ggttaaacgc cagccaaagc cagggcaaac gccatgacat cattcagctt agtggagaaa
    13021 atctggctgc agggttaaat ggcgagtcac tattcctgtt cgccggtgat cagaaagacg
    13081 ccgatgcgat ttatgccaat ccactgctcg cgcacctgcc cgcagtacaa aacaagcagg
    13141 tttatgcgct gggaaccgag acgttccgcc tggattacta cagcgccatg caagtgctgg
    13201 aacgacttaa ggcgctgttt taagcattaa ctgtcggacg ctgtcacctg cggcggcgtc
    13261 tggcgaaaac gtcgcaactc caccagcacc agcaataaca acacgccgat aatcaacaaa
    13321 ccaaaaccgc tcgcgcttgc ggaagcaacc ggtgtcatca tcgctcccag accgcctaac
    13381 agcgccgcgc ctatggcatc gcccgtcacg ttttgtgccg tccacaaacc gttgattcgc
    13441 cctaacattg cttccggcgt ttgcgtttgc agcattgtat attgcagcaa cgaactgacc
    13501 gcactcagcc agccgaacag cgccagacaa accacgccta aaatccacat cggcatcagg
    13561 ccaaataaac caatggcgag gaacgatccc aacgtggaga gcaacatcaa caatcctggt
    13621 cgcgcgctat gcgccagttt cccactggtt aatgcgccaa tagccgcacc gagcgggatc
    13681 gccgcataga gaaaaccaat ctctgccgcc gacatctgcc agttgtcagc cagcgccgga
    13741 tacagtaccc gcaccgcgct cgccatcgtc aataaaccac ccagcaacgc aatcccgccc
    13801 accagcgggc tggcgagcag aaaacgaaat cctgccagta atgatttcaa cggatgctcg
    13861 cgcggctgcg gtggcggtgg caacgccgga aggcttaaca acggtagcaa ggtaataaac
    13921 gtgcccgccg ccgccagccc gtagttccag gctacgccac cggtcgccag caataaaccg
    13981 ccaatcatgg gcgaaatcac cgaccccaga cgcacggtca acatggtgat cgccccggcc
    14041 tgcattaagt tttcacgccc taccagtgct ggtgtcgccg ccaatagcgc cgtaacgcca
    14101 agcgatgcga aaaaaccatc ccataaacca agtaaataga ttgccagcaa tgacggctcc
    14161 ggcagcagtg cattaaggca cagtccaatg aagccaatgc cacaggtgcc gcgcgccagc
    14221 aaaatcactt ttttgcgctc atagcgatcc gccagcacac cgccaaccat caggccaaca
    14281 aacatcgcgc cgccggtcag cgtcaccgaa agccccacca gccaggtaga atgcgtcatc
    14341 atctggatct gcaccggcac cgcgacgccg agcaaaccca gagacacaat tgagataaaa
    14401 cgagcgagga atactgcgcg aaacgccggg tgcgttttca acaggctgag gttaagcagc
    14461 caggattgtt tattcattac aatgccttgc catcaaatat tttaaatacc tattcttagg
    14521 ctgcacatgc taacatatcc aaataagatc gataacgata attaatttca ttatcatgga
    14581 agttcgtatg tctggttctg ttgccgtgac acgcgccatt gccgtgcccg gattgctgtt
    14641 attactgatt atcgcgacgg cattaagcct gctcattggg gcaaaatcac tccccgcttc
    14701 cgtagtgctg gaggccttct ccggcacctg ccagagcgcc gactgcacca tcgtgctcga
    14761 cgcgcgactg ccgcgcaccc ttgccggttt actggcaggc ggcgcgcttg gccttgccgg
    14821 ggcgttgatg caaaccctca cccgaaatcc acttgccgac cccggcttgc ttggcgtgaa
    14881 cgccggagcc agctttgcca ttgtgctggg tgcggcgctg tttggttact catccgcgca
    14941 ggaacaactg gcgatggcct tcgccggggc gctggtggcc tcattgattg ttgcctttac
    15001 cggcagtcag ggcggcgggc agttaagtcc ggtgcgttta accctggcgg gcgtggcgct
    15061 ggcggcagtg ctggaagggc tgaccagcgg catcgccctg cttaatcctg acgtctacga
    15121 tcaactgcgt ttctggcaag ccggttcgct ggatattcgc aatctacata ccttaaaagt
    15181 ggtgctgatc ccggtgctga tcgccggagc aaccgcgcta ttactgagcc gcgcgctgaa
    15241 cagtttaagc ctcggcagcg acaccgcgac ggcgctgggc agtcgcgtgg cgcgcacaca
    15301 gttaattggt ctgctggcga ttaccgtgct ttgtggtagt gcgacagcgg tagttggccc
    15361 gattgccttt attggcctga tgatgccgca tatggcgcgt tggctggtgg gtgccgatca
    15421 tcgctggtcg ctgcccgtaa cgctgcttgc tacccctgcc ctgctgctgt ttgccgatat
    15481 catcgggcgg gtgattgtgc ccggtgaact gcgcgtttct gtggtcagtg cctttattgg
    15541 tgcaccggtg ctgatcttcc tcgtgcgacg taaaacgcga ggtggtgcat gatttacgtc
    15601 tctcgccgat tactcatcac ctgtttgctg cttatttccg cctgtgtggt tgcaggtatc
    15661 tggggattac gcagcggtgc cgtcacgctg gaaacctcgc aggtattcgc cgcgctgatg
    15721 ggcgatgcgc cgcgcagtat gacgatggtg gtcaccgaat ggcgtttacc acgcgtgctg
    15781 atggcgctgt tgattggcgc ggcactgggc gtcagcggtg cgatttttca gtcgctgatg
    15841 cgtaacccgc tcggcagccc tgacgtaatg ggctttaaca ccggggcgtg gagcggcgtg
    15901 ctggtggcga tggtgctgtt tggtcaggac ctgacggcta tcgcgctggc agcaatggtg
    15961 ggcggcattg tcacttctct gttggtctgg ctgctcgcct ggcgtaacgg tatcgacacc
    16021 tttcggttga ttattatcgg catcggcgtt cgcgccatgc tggtggcctt taatacttgg
    16081 ctgttgctga aagcgtcttt agaaacggcg ctaacagcgg gtttgtggaa tgccggatcg
    16141 ctcaatggcc tgacgtgggc aaaaacttcg ccttccgcac ccatcattat attgatgctc
    16201 attgccgccg ccttgctggt gcggcgaatg cgcttgctgg agatgggcga tgataccgcc
    16261 tgcgcgctgg gcgtcagcgt cgagcgttcg cgtctgttaa tgatgctggt tgcagtggtg
    16321 cttaccgctg cggcaacggc gcttgccggg ccgatttcct ttattgcttt agtcgcgccg
    16381 cacattgccc gacgcattag cggcaccgct cgctgggggc tgacccaggc ggcgctgtgc
    16441 ggagcgctgt tactgctggc ggccgatctc tgcgcccaac aactgtttat gccgtatcaa
    16501 cttccggttg gcgtcgttac cgtcagcctc ggcggtattt accttatcgt cttgttaatt
    16561 caggagtctc gcaaaaaatg accgaatcag tagcccgttt gcgcggcgaa cagttaaccc
    16621 tgggatatgg caaatatacc gttgcggaaa atctgactgt agaaattcct gatggtcact
    16681 tcacggcaat tatcggacca aatggctgtg gtaaatccac gttactgcgt accttaagcc
    16741 gcctgatgac gcctgctcac gggcatgtct ggctggatgg cgagcacatt aaacattacg
    16801 ccagtaaaga agttgcacgc cggattggtc tgttggcgca aaatgctacc acgccgggcg
    16861 atatcaccgt gcaggagctg gtggcgcgtg gacgttatcc gcatcaaccg ctgtttaccc
    16921 actggcgcaa agaggatgaa gaagcggtaa cgaaagcgat gcaggccacg ggaataactc
    16981 atctggcaga tcaaagcgtg gatacccttt ctggcggaca acgccagcga gcgtggatcg
    17041 cgatggtgct ggcccaggaa acggcaatta tgctgctcga cgaacccacg acctggctgg
    17101 atatcagtca tcagattgat ctactggaac tgttaagcga actgaaccgc gagaaaggct
    17161 ataccctggc ggcggtgctg cacgatctta atcaggcctg tcgttacgcc agccatttga
    17221 ttgcattgcg ggaagggaaa attgttgctc agggagcgcc gaaggagatt gtcactgctg
    17281 aactgattga gcgcatttat ggtctgcgat gcatgatcat tgacgatccg gtagccggaa
    17341 cgccgcttgt ggtgccgctc gaacgaacgg caccttcaac cgcaaatagt taaactaagt
    17401 ggtctgccat catggcatcc tgttttctgg atgccatcgc atagcgcaat aacacgctac
    17461 cacaagccac catcccgccg atcagcgcgg aaaggatcac aataatcgcc ttacccggac
    17521 cgtctttttt cactggcaat gacgggctta actgatattt aaacggcgta aaattcacat
    17581 cgttgacatt tgcttttgtt aattgctcga caagatactg ccgattacgt aattcaccgt
    17641 tcagttccgc aacgtcagta accgcttttt caatttccag tttgcgttca ataccgtctg
    17701 cgccgagaga aatagagaaa tcggggtcat ctttaacggc ctgaccatta ctgtatacgg
    17761 gctttttaat tcctgccgcg ttggcaatgt cgagtgaata attgaggcgc tgaatgtttg
    17821 catcaagttg atttttcgtt ttaatgcgat cctgagccag tttttctttt tcaaactggg
    17881 ttttgatctc cagtttatta cggacgtttt ctatcgactc tttcaccacc aacgcagaga
    17941 tataatcgat atacccgctc aaaacggtct gcgcctcttt acttgttggc gcggtaaaac
    18001 ttagcgtcca ggaggtatac agtgacggtt catctttttt cttactggca ttgtcatcaa
    18061 ccgctttcat tttttcgctc aatgcaacaa tagcgcgatg caaatccagt tcatcgattt
    18121 tcgcctcttt taattggtcc atcacataag gtgatgaacg caggtactct tccagcaagc
    18181 taacagactg aaacttcttg ataaacaggt taaatgcacc gccacggtcg atattaacat
    18241 cgagatcaag aacgcggagt ttggtaaagg ttttctccag gtcctgccac tgaatggctt
    18301 cggcgggagt aataacggct gagctggtcc atttttgcgg caggatgaaa gagatcagta
    18361 aacctgcgca ggcaaatgca aaaacgaccg ccatgaccgt ttttttggcc cgccataaaa
    18421 cttcgattag attaagtaaa tcaatttcat tactactggg cgacgccaga ggatactcgg
    18481 aaaaatgagc gtcacttccc tgtttaatat tcagtgatga catgggtata acctgaaaat
    18541 gagtccaata gggggaaatc tccacggcgc tcactttacc agttggtgag aattatccga
    18601 agctgaagtt tgtaaattcc ttccactgaa tgcaacattc actctattta agagaaatat
    18661 tcatcaaacc tataaatcaa acaaataaaa aacccataat tattttctgt aattatgggt
    18721 ttatagataa tattaattta cctgtttagc gttgcgcgaa taatcggccc aatttttaca
    18781 aacgcccctg gagagataat atccacatgc gcacaatcct gacgatagat atccagctcg
    18841 gcaatccagg gcgaccaagc gcgttcgggg ctcatacctt cctgaagcgt gcgttcagca
    18901 acaaacagcg tcgctttacc atcaaacggt acgctatgag cagtcgtcag cagacgcaca
    18961 gcatcagcgt agttgccttc aatggtggta aacaactccg ttgaagtact tccctgctgt
    19021 gccgccagga aggcctcgcg ttcgcggtta atctccgcca gcacttccgg gtccagacca
    19081 ttagcctctt tttcctgcca gttttgcgtt tctggcggcc aggtatccag caaaccaaga
    19141 aatgccacct gttcgccacg ggcacgcagt cgcgccgcaa tgccctgcgc cagcgtaccg
    19201 ccaagggaat accccagcag gtaataaggg ccgtgcggtt gttgttcaag taacgttgcc
    19261 agatgcgctt cacagacttc atccagattt gccgcggtat gcatggggcc attggggcgc
    19321 ggcgactgaa tgccgataat cgaccattgt ggatcgatat aacgcgagag cacgctaaac
    19381 tgccaggcaa aaccggatgc cggatggaaa caaaacagcg tcgggccatt accttcacgc
    19441 aacggcagaa tggtttcgaa tcccatacgc cgggtgctgt cttcttcagc atcaataatc
    19501 gttgccagtt tagcgacggt tgacgcgacc atcacttgcc ccggcgtcac ctggcgggca
    19561 acctgccgac ttaactgcgc tgccagtttc attgccagta gcgaatgacc accaagcgca
    19621 aagaaatcag catcggcatc ctgcacgtca caacccagca acgacgcgaa tgccgcggca
    19681 ataatcgttt cactgcccgc ttttggcgca cgccccggcg cttgtgcctt cagttcaggc
    19741 aacggtaagg ctttgcgatc cagcttgccg ttggcgctaa gaggtaacag tggaagttgc
    19801 agcagaacca cgggcaccat atgtggtggc aatgtttcgc gaagctgcgc ctgtaatgcg
    19861 ctggtatcca acggcagacc tgattgcgac accagatagc ccaccaactg acgcgcatca
    19921 ccaccggtgg ctgccgcctg gttaatcaca caggcgtggg taacggcttg ttcgacatcc
    19981 ggcagcgcct gcatcacgcg atcgatttcg cccagttcaa tacgctgccc gcgaattttt
    20041 agctgatcgt cactgcgccc gaggtactcc accgcgccgt tatccagcca gcgggcaacg
    20101 tctccggtac ggtacatccg ttcaccaggg acaaaaggat cggcaataaa gcggctggcg
    20161 gtcagatcgg gtcgtccaag atacccctgc gccagttgaa tgccggtgag atagagatca
    20221 cccgccacac ccggcggcac cggatgcatc atcgcatcaa gaatacgcag acccgtattc
    20281 cataccggat aaccaatcgg cacactgctg ccgcgcacct gtgccagttc ctcgccaaaa
    20341 gccggatacc agctgacatc taccgccgct tccgtcgggc catatagatt atgcaacggc
    20401 acgctggtta actgttgcca ttcgcggcat aaatcagccg gtaaggcctc accactacag
    20461 aaaacctgtt tcaacgtcgc gcaactctgg cgagcggttt gcggcgtcag cgatgcaaca
    20521 aatgctgcca gcatcgacgg cacaaagtgc gtggtcgtta cgccatattc ggcaaagaat
    20581 tgctgcatag cgagcgggtc gcggtgcgct tccggttcag ccatcaccag tttagccccg
    20641 gcaataaacg gccagaaaaa ctcccacacc gagacatcaa aactgcacgg cgttttttgg
    20701 gcaacgacat cttcgcctgt aagcagataa tgattttgca tccacaacag gcggttaacg
    20761 atagccgtct gcccgaccat tacccctttc ggcctgccgg tggagccaga ggtaaagatg
    20821 atataagccg tgtgatgcgg ttgtgaaagt tgcagcggcg cactgccctg cggtttaagc
    20881 ggggcgttat agcaaaggtt ggttaaatct ggaacatcgg caaagcgcgg cagttgatcg
    20941 tcggtggtga ttaacagcga cggacgcgca tcttccagca tcattttcag gcgatcgtcc
    21001 ggatagccgg tatccagcgg tagccaggcc gcgccagctt caactatcgc atgtagcgcc
    21061 agggtcaaaa agaccgagcg cggtagcgcc accgccacgc tgtcgcccgg tttaacgcca
    21121 cgctcacgca gcagattcgc cagcgccacc acctgctcgc gcatttcccg atagctgaac
    21181 tggtaacgcg catctgccag cgccggagca tccggtgttt ttgccgcttg ttctgccacc
    21241 agcgcgctca gcgtggtttc tggaatctca acctgagtgg cgttgatctg cgccagctgc
    21301 gcatactcac ctggcagcat aatatcgaca tcgccgcaca acagcgccgg atccgcggcg
    21361 aactgggcaa tcagcatttt caggcgttca gcatgctgga ttaacgttgg ctcatcgtaa
    21421 cgctgtttat tggcgaggat ctcaatactc aaatcaccgt gttcatccgg gaacagggcc
    21481 agttcaaggt cattaaccgg accggttgcc aaggtatggg tttgcgcctg aacaccagga
    21541 atatccagtt ggtaatcaaa taccttgata ttgagtaccg gaccaaacag cggttcatca
    21601 cctgccgctc gcccgctgtc acggacaatt tgttcggcat cgtaacgttg atgacgacgc
    21661 atttttttca gttgtgctgc cagtcgggtt gccagttccg gcagcgtttc ttgcgccgca
    21721 atgtgaatac ccaacggcaa aacgttgagc acgggtccgg tagccgtcag cgccgccgag
    21781 cccagtcgac gcataaagat aaatccagcg gcgtagtcca tgcgattgca caatcgcccc
    21841 agccacaaag ccgtcagcgc aagggctaaa tcggtacgct gcacacctga aagttgcgta
    21901 gccaactggc ggaattcccc gtcggtaaat tccattttca gacgtaaaat atctgccgaa
    21961 gcgctgcgcc ccggtaaagg tgccggagaa agtgacgcgg gcggcggcag ttgacgacgc
    22021 tgttctgccc agaatgccgc atcacgctgc caggcttcgc tttcgcggta ttgctggtac
    22081 tcttccacta catcagcgaa aggcgtaaac ggcgaagcag gcgttggttc accacgcagc
    22141 catgtgcagt aaatattggc gatctggcgg gtaatggccg ggaaactgaa gccatcgacc
    22201 agcaaatggt gataacgctg ataccagtac cagcggttat ccgccacctg tatcagctga
    22261 tgaaagacca gtggtttacc gctatcgacg cgcagatctt gttgcaaatc cgcctgcatt
    22321 aatgcctgcg cagtaccgtg cggatcaatg ttggttcgca ggtcgataat ttctggcagt
    22381 tcgaacgtca gcgcatcatc gacccactgc cagacttcgc cgttatcttc cgtaaaacgc
    22441 atccgcagcg tatctgcttg cgctagtccg tcaaccaccg cgcgggccag taatggcgca
    22501 tcaacctctc cggttaactc aacgtaatgc gctacgctcc aggcggaggg taaatccgac
    22561 agtttttctg ccatccagat gccgggctgt gcggcgacca aaggtaaatg ctggctcatt
    22621 gcgcctcctg caactgggtg aaattcgccg gtgtcagagt acgccagtgg gcttcaagcc
    22681 actgctggca ggactcttgt gactgcggct gacacacaat gtcccagcct gccggtaagg
    22741 cgcattgttg cggccacaga ctgaattgcc cctgcgcatt gcgcaaaatg taaaacgctc
    22801 cctgtggatc atcgaaggga ttactgaatg ccatattcaa ctcctgtcat ggaaaagtgg
    22861 ttgccagagg tcgattagcc cctgcatcaa gccaccgcgc caacaaagcg catcatgtcc
    22921 gccgtcaacc tggcgccaga aaatggattc ttttatgggg tgtaattgcg catacagcgc
    22981 ctgattggcc cgcataatca tcggctcgcg aatacccgct tccagcacaa tgcgcagacc
    23041 ttcggcgcta acttcaccag ctttaagctt ttcaagtaac acgccctctt gctgcccgcc
    23101 ccgatgcggc caccagtacg atcctgactg gcttaataca caaccaaaac gttcaggcca
    23161 gtgcagtccg gcatacagcg cggaaagtcc accaaaactc tgcccggcaa ccacggtgcg
    23221 atcggcgcga tcgctaaaag gggcaatagc tttcaccagg ggtaataact cttgctgtac
    23281 tgcgagccag aaatccgcat tacacggcag ttcgtgagcg cggtgcgtgg tgtcgatagc
    23341 gtcgatcaac acatacacgg cgggaggaag ttgctgacga tgggtcagcg aagtcagcac
    23401 tggccagacg ggcatacttt gcgcccaaaa ttcgccatcg agcaaaactg ccagcgggcg
    23461 ttcttctgct gttgcatcgc cggtggtaaa aatccataca cgacgtgaat ttttcaaccg
    23521 ttcacttttc cagataattt ctttggctgg tatttctggc gcttgcggac aatcccatcc
    23581 cggttgcaga ggcgcttgcg gcatttcgag tgcagaaaca gcgtgccctc gcccgccttt
    23641 ccagctttgt ggattcagcg gatcggctat tgcctggggt aatagttttc gccagccttc
    23701 acgtaattcg aggcgatcgg gggatggtgc agaaaaaatg tcatcgcgtt cggtgggaat
    23761 aaagcagtag ctgccgcgcc agttggcatt gagttgtgtc gtccactgcc agacatcagt
    23821 acctgcaatg cgctgcatcg actggggctg gctgttctga tgatgatcgg tcacaccagt
    23881 gatgtagacc catacgcgct ttatcggcga gtattcttca gaaccttggg gatcacgcca
    23941 ccagaaagtg acctcaaaca tttcgtcatt cagacgctgc cattccgggc catgtttcga
    24001 ctgccaccag ctctcacttc ctacttttaa cgccgtcaca ccataacccc atgtttactg
    24061 tgcaattttt cattgattgc agaaatatat tgataatatt attgataact atttgcattt
    24121 gcaatagcgt aatggcgcgc cgtgggaagc gcggacatta attaaccaac tgcactgcgt
    24181 gtctttcagg atcaaaggtt ttcgcggtag cgggatgcgt cgtgttgagg acgaccatgc
    24241 ccgacagttg caattcgtgg caaaaatgca ggaataaaac aatgaacaag aagattcatt
    24301 ccctggcctt gttggtcaat ctggggattt atggggtagc gcaggcacaa gagccgaccg
    24361 atactcctgt ttcacatgac gataccattg tcgttaccgc cgccgagcag aacttgcagg
    24421 cacctggcgt ttcgaccatc accgcagatg aaatccgcaa aaacccggtt gcccgcgatg
    24481 tgtcggagat catccgtacc atgccaggcg ttaacctgac cggtaactcc accagtggtc
    24541 agcgtgggaa taaccgacag attgatattc gcggtatggg tccggaaaac acgctgattt
    24601 taattgacgg caaaccggtc agcagccgta actcggtgcg tcagggctgg cgtggcgagc
    24661 gcgatacccg tggtgatact tcctgggtgc cgcctgaaat gattgaacgt attgaagttc
    24721 tgcgtggtcc ggctgcagcg cgttatggca acggcgcggc gggtggcgta gttaacatca
    24781 ttaccaaaaa aggcagcggt gagtggcacg gttcctggga cgcttatttc aatgcgccag
    24841 aacataaaga ggaaggtgcc actaaacgca ccaatttcag tctgaccggc ccgctgggcg
    24901 acgaattcag cttccgcttg tatggcaacc tcgacaaaac ccaggctgac gcgtgggata
    24961 tcaaccaggg ccatcagtcc gcgcgtgccg gaacgtatgc cacgacgtta ccagccgggc
    25021 gcgaaggggt gatcaacaaa gatattaatg gcgtggtgcg ctgggacttc gcgcctctgc
    25081 aatcactcga actggaagcg ggttacagcc gccagggtaa cctgtatgcg ggcgataccc
    25141 agaacaccaa ctctgacgct tacactcgct cgaaatatgg cgatgaaacc aaccgcctgt
    25201 atcgccagaa ctactcgctg acctggaacg gtggctggga taacggagtg accaccagca
    25261 actgggtgca gtatgaacac acccgtaact cgcgtattcc ggaaggtctg gcgggcggta
    25321 ccgaagggaa atttaacgaa aaagcgacac aggatttcgt cgatatcgat cttgatgacg
    25381 tgatgctgca cagcgaagtt aacctgccga ttgatttcct cgttaaccaa acgctgacgc
    25441 tgggtacgga gtggaatcag caacggatga aggacttgag ttccaatacc caggcgctga
    25501 ccggggcgaa caccggcggc gctattgatg gtgtgagtgc caccgaccgt agcccgtatt
    25561 caaaagcaga aattttctcg ctatttgccg aaaacaacat ggagctgact gacagcacca
    25621 tcgtaacgcc ggggctgcgt ttcgatcatc acagtattgt cggcaataac tggagcccgg
    25681 cgctgaacat atcgcaaggt ttaggcgatg acttcacgct gaaaatgggc attgcccgcg
    25741 cctataaagc gccgagcctg taccagacta acccgaacta cattctctac agtaaaggtc
    25801 agggctgcta tgccagcgcg ggcggctgct atctgcaagg taacgatgac ctgaaagcag
    25861 aaaccagcat caacaaagag attggtctgg agttcaaacg cgacggctgg ctggcgggcg
    25921 tcacctggtt ccgtaacgat tatcgcaata agattgaagc gggctatgtg gctgtagggc
    25981 aaaacgcagt cggcaccgat ctctatcagt gggataacgt gccgaaagca gtggttgaag
    26041 gtctggaagg atcgttaaac gtaccagtta gcgaaacggt gatgtggacc aataacatca
    26101 cttatatgct gaagagtgaa aacaaaacca cgggcgaccg tttgtcgatc atcccggagt
    26161 acacgttgaa ctcaacgctg agctggcagg cgcgggaaga tttgtcgatg caaacgacct
    26221 tcacctggta cggtaagcag cagccgaaaa aatacaacta caaaggtcag ccagcggttg
    26281 gcccggaaac caaagaaatc agtccttaca gcattgtggg tctgagcgcg accagggatg
    26341 tgacgaagaa tgtcagtctg accggcggcg tggacaacct gttcgacaaa cgtttgtggc
    26401 gtgcgggtaa tgcccagacc acgggcgatc tggcaggggc caactatatc gcgggtgccg
    26461 gggcgtatac ctataacgag ccgggacgta cgtggtacat gagcgtaaac actcacttct
    26521 gatgctaacg tcagattgtt gataaagtgc gcgtcgttca tgccggatgc ggcgtgaacg
    26581 ccttatccgg cctacaaaaa tcttgccaat tcaatatatt gcaggaccat gtaggcctga
    26641 taagcgtagc gcatcaggca gtttagcgtt tgtcaccagt ctctaatatg gtcgacatga
    26701 aaactacgca tacctccctc ccctttgccg gacatacgct gcattttgtt gagttcgatc
    26761 cggcgaattt ttgtgagcag gatttactct ggctgccgca ttacgcgcaa ctacaacacg
    26821 ctggacggaa gcgcaaagcc gaacatttag ccgggcggat cgctgcaatt tatgcgctgc
    26881 gggaatatgg ctataaatgt gtgcccgcaa tcggcgagct acgccaaccg gtgtggcctg
    26941 cggaggtata cggcagtatt agccactgcg gggctacggc attggccgtg gtttcccgtc
    27001 aaccgattgg cgttgatatc gaagaaattt tttctgcgca aacagcgacc gaattgacag
    27061 acaacattat tacaccagca gaacacgagc gactcgcaga ctgcggttta gccttttctc
    27121 tggcgctgac actggcattt tccgccaaag agagcgcatt taaggcaagt gagatccaaa
    27181 ctgatgcagg ttttctggac tatcagataa ttagctggaa taaacagcag gtcatcattc
    27241 atcgtgagaa tgagatgttt gctgtgcact ggcagataaa agaaaaaata gtcataactc
    27301 tgtgccaaca cgattaattg acaacatctg gtacgattcg cccgcagcca tcactgaccg
    27361 cgggcgaaag tgtaaagcag gtgccttacc atcctgacct gacaaccgga tatgcgggga
    27421 ttgctccccg cacaacggct acttcttcgg ttcgtaagcg agaacagcct taaactcaat
    27481 attacgttcc tttacggtga actcacacag cgaatccccg accagaagcg taaatcccag
    27541 caccgttaaa cacagcacta tgactgccgc aagggcatat ttcgtcagca tgtttatatt
    27601 gcctccatgc gaagaaggga ctaccatcca aattgttcag gtttgggtag gtagccccag
    27661 ggttgataga aatatcgtcc tggggcttca tctttccaga cctcagagta aacctgaaac
    27721 cggaaagctt caggcacccg cagttatctt acccttgctt agcaaaatag ctgtttattt
    27781 atacagtaag tcctgtttcg gacttctcac tccccatttg atcaactgaa gctgaacccg
    27841 tttttttctg catgcctcaa tcgtcaaaat tcttcgccac agtcagcact gccataactt
    27901 gtcacattgc aaaagtagcc aaattgcttc tgtcataatc agttcaccat caccaaaaaa
    27961 aagaaacatt ccagaacttc cgcatctcgc ccagccgctc ccacttctat actgaataga
    28021 aaacgccaac ataagagaaa cctatgccat tacccgattt tcatgtttct gaacctttta
    28081 cactcggtat tgaactggaa atgctggtgg ttaatccgcc gggctatgac ttaagccagg
    28141 actcttcaat actgattgac gcggttaaaa ataagatcac ggccggagag gtaaagcacg
    28201 atatcaccga aagtatgctg gagctggcga cggatgtttg ccgtgatatc aaccaggctg
    28261 ccgggcaatt ttcagcgatg cagaaagtcg tattgcaggc agccgcagac catcatctgg
    28321 aaatttgcgg cggtggcaca cacccgtttc agaaatggca gcgacaggag gtatgcgata
    28381 acgagcgcta tcaacgaacg ctggaaaact ttggttatct cattcaacag gtgaccgttt
    28441 ttggtcagca tgttcatgtt ggctgcgcca gtggcgatga cgccatttat ttgctgcacg
    28501 gcctgtcacg gtttgtgccg cacttcatcg ccctttccgc cgcgtcgcca tatatgcaag
    28561 gaacggatac gcgttttgcc tcctcacgac cgaatatttt ttccgccttt cctgataatg
    28621 gcccgatgcc gtgggtcagt aactggcaac aatttgaagc cctgtttcgc tgtctgagtt
    28681 acaccacgat gatcgacagc attaaagatc tgcactggga tattcgcccc agtcctcatt
    28741 ttggcacggt ggaggttcgg gtgatggata ccccgttaac ccttagccac gcggtaaata
    28801 tggcgggatt aattcaggct accgcccact ggttactgac agaacgcccg ttcaaacatc
    28861 aggagaaaga ttacctgctg tataaattca accgtttcca ggcctgtcgc tatgggctgg
    28921 aaggcgtcat taccgatccg cacactggcg atcgtcgacc gctaacggaa gacaccttgc
    28981 gattgctgga aaaaatcgcc ccttccgcac ataaaattgg tgcatcgagc gcgattgagg
    29041 ccctgcatcg ccaggtcgtc agcggtctga atgaagcgca gctgatgcgc gatttcgtcg
    29101 ccgatggtgg ctcgctgatt gggctggtga aaaagcattg tgagatctgg gccggtgact
    29161 aagtagggaa ttgccttaca gaagaatatc cctgacaatg gtgttttaac ttacatttaa
    29221 caatgttatg aaacaccctt tagaaacctt gactaccgca gcaggcattt tgctgatggc
    29281 tttcctctct tgcctgctgc tgcccgcccc cgcactgggg ctgacgctgg cacaaaaact
    29341 ggtgaccatg ttccatctga tggatcttag ccagctttac actttattgt tttgtctgtg
    29401 gtttttagtg ctgggcgcta ttgagtattt tgttctgcgc tttatctggc gacgctggtt
    29461 ctcgctggcg gattaaacgt cgattatcgc cgtacttcgc ggcatacttt gcttattctc
    29521 cttcgcctaa aggaacgttt atggataagc aatcactgca cgaaacggcg aaacgcctgg
    29581 cccttgagtt accctttgtc gagctttgct ggccttttgg cccggagttc gatgttttta
    29641 aaattggcgg caaaattttt atgctgtcgt cggagctgcg cggcgtcccc tttatcaatc
    29701 tgaagtccga tccacaaaaa tccctgttaa atcagcagat atacccgagc attaagccag
    29761 ggtatcacat gaataaaaag cactggattt cggtgtatcc cggcgaggaa atctccgaag
    29821 cgttacttcg cgatctgatc aacgattcgt ggaatctggt ggttgatggt ctggctaaac
    29881 gcgatcaaaa aagagtgcgt ccaggctaaa gcggaaatct atagcgcatt tttctcgctt
    29941 accatttctc gttgaacctt gtaatctgct ggcacgcaaa attactttca catggagtct
    30001 ttatggatat catttctgtc gccttaaagc gtcattccac taaggcattt gatgccagta
    30061 aaaaacttac cccggaacag gccgagcaga tcaaaactct cctgcaatac agcccatcca
    30121 gcaccaactc ccagccgtgg cattttattg ttgccagcac ggaagaaggt aaagcgcgtg
    30181 ttgccaaatc cgctgccggt aattatgtgt tcaacgaacg taaaatactt gatgcctcgc
    30241 acgtcgtggt gttctgtgca aaaaccgcga tggacgatgc ctggctgaag ctggttgttg
    30301 accaggaaga tgctgatggc cgctttgcca cgccggaagc gaaagccgcg aacgataaag
    30361 gtcgcaagtt cttcgctgat atgcaccgta aagatctgca tgatgatgca gagtggatgg
    30421 caaaacaggt ttatctcaac gtcggtaact tcctgctcgg cgtggcggct ctgggtctgg
    30481 acgcggtacc catcgaaggt tttgacgccg ccatcctcga tgcagaattt ggtctgaaag
    30541 agaaaggcta caccagtctg gtggttgttc cggtaggtca tcacagcgtt gaagatttta
    30601 acgctacgct gccgaaatct cgtctgccgc aaaacatcac cttaaccgaa gtgtaattct
    30661 ctcttgccgg gcatctgccc ggctatttcc cctcagattc tcctgatttg cataaccctg
    30721 tttcagccgt catcataggc tgctgttgta taaaggagac gttatgcagg atttaatatc
    30781 ccaggttggg gatttagcgg gtattgagat cgatcacacc acctcgatgg tgatgatttt
    30841 cggtattatt tttctgaccg ccgtcgtggt gcatattatt ttgcattggg tggtactgcg
    30901 gaccttcgaa aaacgtgcca tcgccagttc acggctttgg ttgcaaatca ttacccagaa
    30961 taaactcttc caccgtttag cttttaccct gcaggggatt atcgtcaata ttcaggcggt
    31021 attctggctg caaaaaggca ccgaagcggc agatattctg actacctgcg cgcagttgtg
    31081 gatcatgatg tatgcgctgc tttcagtctt ctcgttgctg gatgttattt tgaatctggc
    31141 gcagaaattc ccggcggcat ctcagttacc gctgaaaggg atatttcagg ggattaaact
    31201 gatcggcgca atcctggtcg gcattttgat gatctcgctg ctgattggtc agtcgccagc
    31261 gattctgatc agcggtcttg gtgcaatggc tgccgtgctg atgttggtat ttaaagatcc
    31321 gattcttggt ctggtggcag gtattcagct ttccgcgaac gatatgctga aactgggcga
    31381 ctggctggag atgccgaaat acggcgcgga tggcacggtg atcgatattg ggttaaccac
    31441 cgtcaaagtg cgtaactggg acaataccat taccactatt cccacctggt ctctggtttc
    31501 tgactccttt aaaaactgga gcgggatgtc agcatctggt gggcgacgta ttaagcgcag
    31561 tatcagtatt gatgtcacca gtattcgttt tcttgatgaa gacgaaatgc aacgtctgaa
    31621 taaagcgcac ttgttaaaac cttatttaac cagccgccat caggaaatta atgagtggaa
    31681 tcgccagcaa ggttctacgg agtcgatatt aaacctgcgc cgaatgacca atattggaac
    31741 ctttcgtgcc tatctgaacg aatatctgcg taaccatccg cggattcgta aagatatgac
    31801 cttaatggta cgccaactgg ctccaggtga taacggttta ccgctcgaga tctacgcgtt
    31861 taccaacacc gtggtgtggc tggaatatga aagcattcag gctgatatat tcgatcacat
    31921 atttgcgatt gtcgaagagt ttggtctgcg acttcatcag tcgccagccg gcaatgatat
    31981 tcgctctctg gcgggtgcat ttaagcagta attaaaaaaa ccgctctcat cgaatggatg
    32041 agagcggaca tcgttttgtc ggatgtagcg tgaatgcctt atttccgacg cagcgtttta
    32101 aatgccacaa acaggaatac aatccacacc ggcagcagga tcgctgacaa gcgcatatca
    32161 tccatcgtgc acatcagcag caaaatcatg ccgaggaagg caatgcaaag atagttgccg
    32221 aacggataaa gcagcgcctt aaactgtgtt tcacgtccct gacgtcgcat cgccgcgcga
    32281 aaacgcagat gcgccaggca gatcataatc cagttcaaca gcagcgttgc taccaccagc
    32341 gccatcagca gaccaaacgc tttttgcggc agcagatagt tgattaacac caccagcgaa
    32401 gtgatcgctc cggaaagcat cagcgagtta atcggcacgc cgcgatggct gacgcgagtc
    32461 aaaaacttcg gcgcattacc ctgcacagaa aggccaaaca gcatgcggct gttagagtaa
    32521 accccgctgt tatacactga cagcgatgct accagaatga cgaagttcag cgcagaagct
    32581 acctcgttgc tgtcgagatt atggaaaatc atcacaaacg ggctactgtt ggatttcact
    32641 tccacccacg gatagagcgc cagtaaaacc accagtgaac agatgtaaaa cagcaggatg
    32701 cgatacacca cctgatttac cgcttttgga atgctttttt ccggatcgcg cgcttcagcg
    32761 gcagtaatcc caatcagctc cagaccgccg aaggagaaca taattaccgc cagcgacaaa
    32821 atcagcccat tccagccggt ggcgaagaaa ccaccgtagc gccagaggtt gtcgatactg
    32881 gctttctcgc cgccgtgacc agaaaacagc agccacaggc caaagccgat cataccgatg
    32941 attgccagca ctttaatcaa cgcaaaccag aactcggttt cgccatataa gcgcacgttc
    33001 accaggttaa cggcgttgat gataataaag aaggcggcag cccaaatcca cgttggaaca
    33061 tccgggaacc agtactgcat atagatgccc gcagcggtca gctctgccat tcccaccagc
    33121 acgaacatta cccagtagtt ccagccagag aggaagcccg caaacggtcc ccagtattta
    33181 taggcaaagt gggcaaatga accggatacc ggctcttcga ccaccatctc gccgagctgg
    33241 cgcataatca ggaaagcgat gatcccggcg acgccgtagc ccagcaatac agccggaccc
    33301 gccatctgaa tcgccgggcc aatgccaaga aacagaccag taccaattgc gccacccagc
    33361 gcaatcagtt gaatatgacg gttatgtaat ccgcgatgaa gcgtcggctc ttgatttgac
    33421 gcagtatctt ccgatacggt tgacgcgttt ttcacgcctt tcccctgtgt gtcttttttg
    33481 ttgaggggca ccttttaaca tttagtgccc atcgtcgcaa gacacaatcc acacggttaa
    33541 acggggtatc ctgcttttat ttccgtaccc gatgtcggtg cagccacatc agcttatacg
    33601 ccgccgggat aataaacagc gacagcaaag gcgcggtgat catgccgcca atcatcggtg
    33661 cggcaatccg gctcatcacc tctgaaccag cccccgttcc ccacagaatc ggcagcagac
    33721 cggcgataat caccgccacc gtcatcgctt tcgggcgcac gcgcagtacc gcgccgtgat
    33781 acaacgcctc atccagcttc tgctcactga atgtttgcgg attattcaac gacggctcgg
    33841 cctctatggc gtgacgtaaa tacatcagca tcaccacgcc aaattcggcg gcgcccccgg
    33901 caagggcaat aaaaccggtg cccgttgcca cagaaagatg aaagcccatc caccacagca
    33961 accagatgcc gcccaccagc gcaaacggta cgctgctgat aatcagcaac gcttcgccca
    34021 cccgacggaa tgccagatac aacagcacga agatgatcat cagtgtcatc ggcaccatga
    34081 gtttcagctt atggttggcg cgctccagta gctcgaactg cccggagaat gccacgctgg
    34141 tgcccggttt taactgcact ttttcaacta tcgctttttg caaatcgtga accaccgaca
    34201 ccatgtcacg atcgcgggca tcgatataaa tccagctcgt cgggcgcgca ttctcggttt
    34261 tcagcatcga cggtccggta gagactttaa cgtcggccac gtctgccagg gtgatttgct
    34321 gcttcatcgg cgtcaggatc ggtagctggc gcagtgcctg cgggctatcg cgccagcttt
    34381 gcggataacg cagattaatg ggataacggg caatcccttc caccgtttcg ccaaccatcg
    34441 ccccgcccac cgcagaagtc acaaacaact gcacatccgc caccgtcata ccgtaacgcg
    34501 cggctttttc acggttaatc tcaacgttga tatagcgccc accttccaga cgctcggcaa
    34561 gcgcagaagc cacgcctggc accgttcgcg ctacttcttc aatttgctca gccatcgcgt
    34621 cgatatccgc cagcacagtg ccggaaactt taatgccgat ggggctttta atgccggttg
    34681 agagcatatc gatacggtta cgaattggcg gcacccacag attcgccagc cccggcagcc
    34741 gcacggtgtt atccagttcc tcaatgattt tgtccatcgt catgccaggc cgccactgat
    34801 cctgcggctt aagctggatg gtcgtttcaa ccatctcgag cggagcagaa tcggtggcgg
    34861 tttccgcttt cccggttttg ccaaataccc gcgccacttc aggtacgctc ataattagct
    34921 tgtcgttttt ttgcagcata ctcgccgcct ctgctgcgga aatccccggc agcgtcgatg
    34981 gcatatacaa caagtcgcct tcattgatct gcggtaaaaa ttccccgcca actttattga
    35041 gcggccagag aaccgtcagc accgaaagcg ctgccaccaa cagcgtggtt ttcggccagt
    35101 gcagtacttt cagcaataga ggatgataaa cacgaatcaa aaagcgattg agcgggttac
    35161 tgctttccgg cggaattttg ccacggatcc agtaacccat caggatcggg atcactacga
    35221 tcgccagcag cgccgcaccc gccatcgcat acgttttggt gaacgccagc gggccaaaca
    35281 gacgcccttc ttgcccttcc agggtgaaga ttgggataaa cgacagcgtg ataatcagca
    35341 gactgataaa cagcgccggc cccacttcaa cagatgcatc ggtaatcacc tgccagcgcg
    35401 ttttattatc cagcgtggcg tcaggatgct ggtgctgcca ctcttccagc cgtttatgcg
    35461 cattctcgat catgacgata gcagcatcga ccatcgcccc gacggcaatc gcaatgccac
    35521 ccaacgacat aatattggca ttcagtccct ggaagggcat gacaataaaa gcaatacaca
    35581 accccagcgg caacgaaata atcgccacca gcgccgagcg cacatgccag agaaacagcg
    35641 cacagactac cgccaccaca ataaactctt ccagcaactt gccgctgagg ttgtcgatgg
    35701 cgcggtcaat gagctggctg cgatcgtatg ttgtaactat ctccacgcct tccggcagac
    35761 tacttttcag cgtttccagt ttgtccttca cggcggcgat cacttctcgg gcgtttttgc
    35821 cggagcgcag gatcaccacc ccgcccgcca cttcgccttc gccgttcagt tcggcaatgc
    35881 cccggcgcat ctccgggcca acctggacct tcgcaacatc gcgcagataa acgggcacgc
    35941 cattttcact ggcttttaaa acgatgtgat taaagtcgtc gagcgtttgc agatagccgc
    36001 tggcgcgcac catatattcc gcttccgcca gttcgatcga cgaaccgccc gcttcctggt
    36061 ttgaagcatc cagcgcgctt tttacttcgg cgagactgat gccatactgc gccaggcgct
    36121 ggggatcgat aaccacctga tactctttca ccacaccgcc caccgacgcc acttccgcaa
    36181 cgtcagggat ggttttcagc tcatatttga gaaaccagtc ctgtaatgag cgtaaatcgg
    36241 ccagatcgtg cttaccgctg cgatccacca gtgcatattc atagatccag ccaacacccg
    36301 tggcatctgg ccccagctcg gcgctgactc ccgcaggcag cttaccctgt acctggttga
    36361 ggtactccag cacccgcgaa cgcgcccagt acggatcggt accatcttcg aaaatgacat
    36421 acacataaga gtcgccaaac tgcgagaaac cacgcacagt cttcgcgcca ggcaccgaca
    36481 acatggtggt ggttagcgga taagtcacct gattttcaac gatttgcggt gcctgcccgg
    36541 gatagctggt tttaataatc acctgcacat cggagagatc cggcagcgca tccactggcg
    36601 tattaatgat ggtccaggtg ccccagatgc taagaaacaa cgcgcccatc agcaccaaaa
    36661 aacggttcgc caccgagcga cgaataatcc attcaatcat tggttattcc ctcaatgcgc
    36721 atgggtagca ctttcagagc gcatccgctc cagtgcgcca gaaatattgg cttcggaatc
    36781 aatcaggaaa aggccgctgg aaaccacctt ttcaccttcc gccagaccag agcgtaatgc
    36841 ggtgacacct tgcgatgcct ggaaaacggc aacgcgtttc ggtacaaagc gcccgtcggc
    36901 atcaatggta atcacccgct gttcgctacc ggtatcaatc agcccttgtg acggaatgag
    36961 cagcatcggt tcgctggcgg tattgagttg caaccaggcg ttcatgcccg gttttagcgc
    37021 ctcgtcggcg ttgtcgactt ccagacgcag ctgcagcgtg cgggtcgcgg catccacgcc
    37081 gggtagcagc gtccatttgc ggatggtgag tgttttatcc ggtcgtgccg gaacggtgag
    37141 cgtaaactgc gaggcatctt tcaccagcca ggcgatagac tccgggatcg cagcagtgac
    37201 ccacaccggg tccataccct gaattttcgc taccacgtta tctttggcga tattcattcc
    37261 cgcgcgcaga tcaaacgcgg tgatcacgcc atcaatgggc gctttgagcg taaagcgagt
    37321 ctggattttt tgcgtggcga tcaggcggcg aatatccgcc tccggcattc ccgccagtcg
    37381 cagccgctca agaatgcctt cagtctgggt cgccgtaccg ccggtttcgc gcagcagtaa
    37441 atactcactc tgcgcttcca cccagtcagg aatggtcagg tcgagaagcg gtgtgccctt
    37501 ttgtacttta tcgcccacgg taagcggata caccttgtcg ataaacccgg cagcgcgagc
    37561 ctgcacaatg gcatactgat actcgttgta actgacattc gccgggaaac tctgggcaaa
    37621 agtcagcggt ccgcgcgtga cggtagccgt tttcaccccc agattctgcg tctgagtcgg
    37681 gtcaatgcgc acaccagacg cagaactctc ttcatcggca tatttcggca ccagatccat
    37741 atccataaac ggcgatttac ctggtttatc gaaccgcgta ttggggtaca tcgggtcgta
    37801 ccagaataag attttacgtt
//
LOCUS       DADLFA010000015        37233 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000015 DADLFA010000000
VERSION     DADLFA010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37233
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.015"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..808)
                     /locus_tag="JQH65_000670"
     CDS             complement(<1..808)
                     /locus_tag="JQH65_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HAY6138173.1"
                     /translation="MTIEKSRFSSGAAPQPNVNTGVINGNDFAAIVPVIAGRIGGREA
                     NIVSAKALHKALGVGRDFTNWIKGRIDQYGFIAGTDFIRVENLSTPKRASAKTRQQIE
                     HDYLLSLDMAKEVAMVERNEQGRAIRRYFIQCEEELQRSVPEIAARYRRQLKARLSAA
                     NNFKPMCDALNMARAEMGKTTQQRHYSNESNMIARIVLGGMTAKQWAQANGITGEPRD
                     SMNAAQLEHLAYLESTNITLIDMGMEYEQRKGELTRLSQRWLAKRLEVNHA"
     gene            complement(822..1253)
                     /locus_tag="JQH65_000671"
     CDS             complement(822..1253)
                     /locus_tag="JQH65_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6138174.1"
                     /translation="MEKKNRPLQAANSDIRVSDVTPLTKSLQAPKRTPKKHRARVYML
                     RTGIEGWTENDILRYCRLSSGRNYASELERRLDIQLERIDEKNPDGIGSHLRYRFTGR
                     GDVLKVIRLVNHNADAGGYQGLSQPEIADILNLYPDNLTAA"
     gene            complement(1253..1456)
                     /locus_tag="JQH65_000672"
     CDS             complement(1253..1456)
                     /locus_tag="JQH65_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HAY6138175.1"
                     /translation="MNIDTIPTKGYIRRFRLAELLGVSVSTIDRKVRNGSLPRPVKLG
                     EKITAFDAVEIHNWLAERRGKVA"
     gene            complement(1569..2417)
                     /locus_tag="JQH65_000673"
     CDS             complement(1569..2417)
                     /locus_tag="JQH65_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6138176.1"
                     /translation="MKKLPPAPFFRLNRAAELLEIDIDDLLIMAAAESIYLSAYLCGY
                     DGTLHSIASLYRNCSLFPDVKEDCLLLADIITEVEDEKSFKAGVVNHIDCHVLGLWHV
                     NASYILDIAGLDSIDTPARIFSPAHRKRELDVSANYYFFLDYTKHAFHEISVEDLYIT
                     RPWVEYIGECMDKNQPIYSPETRINLQALYEDVKNVEHGNRERHRQKREICLLALIYV
                     KRHYPEECKGKNGKETNEAWANATINHWALLGGGYDEPSIDYLKKLIADADRTPEERT
                     TAGKKR"
     gene            complement(2513..3772)
                     /locus_tag="JQH65_000674"
     CDS             complement(2513..3772)
                     /locus_tag="JQH65_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY6138177.1"
                     /translation="MPKLTDMQIRAWIKSGERFEGRADGNGLYLRYREADKTPTWRFR
                     YKLAGKSRAMLIGSYSELSLSKARETAKELSARVALGYDVAGEKQKRKTEALAKMEAE
                     KNAMRVSELAAEYFERQILPRWKHPDILRRRIDKDINPCIGSMKVEDVKPRHIDDMLK
                     GIVDRGAPTIATDVLRWTRRIFDYGIKRHALEINPCSAFEVADAGGKEAARDRWLTRD
                     ELIQLFKAMRTAKGFSRQNEITFKLLLALCVRKMELCAARWEEFDLDGAVWHLPEERS
                     KNGDPIDIPLPSPAVEWLRELHTFSCNSAWVLPARKMQNRMIPHIQESTLPVALAKVR
                     AEMPDVPNFTIHDFRRTARTHLAALGVDPVVAERCLNHRIKGVEGIYNRHQYFDERKA
                     ALAQWADLLVALESGKDYNVTPLRRAN"
     gene            complement(3963..4826)
                     /locus_tag="JQH65_000675"
     CDS             complement(3963..4826)
                     /locus_tag="JQH65_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAY6138178.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDISAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            4953..5669
                     /gene="rph"
                     /locus_tag="JQH65_000676"
     CDS             4953..5669
                     /gene="rph"
                     /locus_tag="JQH65_000676"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAY6138179.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            5735..6376
                     /gene="pyrE"
                     /locus_tag="JQH65_000677"
     CDS             5735..6376
                     /gene="pyrE"
                     /locus_tag="JQH65_000677"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAY6138180.1"
                     /translation="MKPYQRQFIEFALGKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGLEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(6413..7009)
                     /gene="slmA"
                     /locus_tag="JQH65_000678"
     CDS             complement(6413..7009)
                     /gene="slmA"
                     /locus_tag="JQH65_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HAY6138181.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(7116..7574)
                     /gene="dut"
                     /locus_tag="JQH65_000679"
     CDS             complement(7116..7574)
                     /gene="dut"
                     /locus_tag="JQH65_000679"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAY6138182.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(7552..8772)
                     /gene="coaBC"
                     /locus_tag="JQH65_000680"
     CDS             complement(7552..8772)
                     /gene="coaBC"
                     /locus_tag="JQH65_000680"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HAY6138183.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEGLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSENNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            8944..9612
                     /locus_tag="JQH65_000681"
     CDS             8944..9612
                     /locus_tag="JQH65_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAY6138184.1"
                     /translation="MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            9829..10065
                     /gene="rpmB"
                     /locus_tag="JQH65_000682"
     CDS             9829..10065
                     /gene="rpmB"
                     /locus_tag="JQH65_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAY6138185.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARSEKY"
     gene            10086..10253
                     /gene="rpmG"
                     /locus_tag="JQH65_000683"
     CDS             10086..10253
                     /gene="rpmG"
                     /locus_tag="JQH65_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAY6138186.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            10351..11160
                     /gene="mutM"
                     /locus_tag="JQH65_000684"
     CDS             10351..11160
                     /gene="mutM"
                     /locus_tag="JQH65_000684"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HAY6138187.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(11199..11678)
                     /gene="coaD"
                     /locus_tag="JQH65_000685"
     CDS             complement(11199..11678)
                     /gene="coaD"
                     /locus_tag="JQH65_000685"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAY6138188.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(11686..12963)
                     /gene="waaA"
                     /locus_tag="JQH65_000686"
     CDS             complement(11686..12963)
                     /gene="waaA"
                     /locus_tag="JQH65_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HAY6138189.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQVLLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            13376..14434
                     /gene="rfaQ"
                     /locus_tag="JQH65_000687"
     CDS             13376..14434
                     /gene="rfaQ"
                     /locus_tag="JQH65_000687"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HAY6138190.1"
                     /translation="MDKLFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLRVDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            14431..15555
                     /locus_tag="JQH65_000688"
     CDS             14431..15555
                     /locus_tag="JQH65_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAY6138191.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     YPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            15548..16345
                     /gene="rfaP"
                     /locus_tag="JQH65_000689"
     CDS             15548..16345
                     /gene="rfaP"
                     /locus_tag="JQH65_000689"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HAY6138192.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWSAIHRLRDVGVDTMYGVAFGEKGI
                     NPLSRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            16361..17377
                     /gene="waaO"
                     /locus_tag="JQH65_000690"
                     /gene_synonym="rfaI"
     CDS             16361..17377
                     /gene="waaO"
                     /locus_tag="JQH65_000690"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HAY6138193.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAIVSRFTY
                     MDQDILNLILLGKVKFIDAKYNTQFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYW
                     AGYPSAQPFIKAKEASPWKNEPLMRPVNSNYARYCAKHNFKQNKPINGIMNYIYYFYL
                     KIIK"
     gene            17394..18389
                     /locus_tag="JQH65_000691"
     CDS             17394..18389
                     /locus_tag="JQH65_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide 1,2-glucosyltransferase"
                     /protein_id="HAY6138194.1"
                     /translation="MNEFIKERFSYLADNKKENAPELNVSYGIDKNFLYGAGVSISSV
                     LINNSDINFVFHVFTDYVDDDYLKSFNETAKQFNTSIIVYLIDPKYFADLPTSQFWSY
                     ATYFRVLSFEYLSESISTLLYLDADVVCKGSLKPLTEIIFKDEFAAVIPDNDSTQAAC
                     AKRLNIPEMNGRYFNAGVIYVNLKKWHEANLTPYLLKLLRGETKYGSLKYLDQDALNI
                     AFNMNNIYLAKDFDTIYTLKNELYDRSHRKYQQTITDKTVLIHYTGITKPWHSWAGYP
                     SASYFNIAREQSPWKKYPLKEARTVAEMQKQYKHLFAHGEYIKGITSLIKYKLKK"
     gene            18399..19091
                     /gene="rfaY"
                     /locus_tag="JQH65_000692"
     CDS             18399..19091
                     /gene="rfaY"
                     /locus_tag="JQH65_000692"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HAY6138195.1"
                     /translation="MITSIRYRGFSFYYKDNDNKYKEIFDEILAYNFKTVKVLCNIDD
                     TKVSLIDTKYGRYVFKVFAPKTKRNERFLKSFVKGDYYQNLIVETDRVRSAGLTFPND
                     FYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPENVKAEIKASMEKLHALNMLSGD
                     PHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTK
                     LRKFIKKLKGKA"
     gene            19117..20142
                     /locus_tag="JQH65_000693"
     CDS             19117..20142
                     /locus_tag="JQH65_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactose--(galactosyl) LPS
                     alpha1,2-galactosyltransferase"
                     /protein_id="HAY6138196.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVIIYKVSNAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDEDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCNFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKYQHAKKNGDTFNAFIYFIKFKLNK
                     YKRKLFG"
     gene            20227..21210
                     /locus_tag="JQH65_000694"
     CDS             20227..21210
                     /locus_tag="JQH65_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAY6138197.1"
                     /translation="MSNDYPLVSIIIPTYNSSDYITETLTKLEKQTYPNFEIVIVNDG
                     SKDNTSNVLREYGLTHSRLIIVNKENGGVSSARNTGIRKAQGQFICFMDDDDEIDPNY
                     LLKMYTRQHETGGDAIYCGLYGHYIKDGVTYSPINTEFNEGSLLFDFFYKKVRFHIGC
                     LFIRKQLLEENNLFFDEDLRLGEDLDFIYRLLITCDMYAVPYYMYKHNYRENSLMNSC
                     RTITHYRHESFAHERIYSSVMQLYKGNRKEEIHTLLSQNRAYHKTRYLWNVLLNGDFK
                     LLNQLVESNEKELRYCNLPGKRDKRRAKILASKNYILWRMVRLVNRKKNKR"
     gene            21256..22509
                     /locus_tag="JQH65_000695"
     CDS             21256..22509
                     /locus_tag="JQH65_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAY6138198.1"
                     /translation="MSFCWNEINSGIKSLILILCIFSLMTLSLWDDVATKFLHAAGII
                     SALYFLATPKKTITNNPTLLIFISLCLLGIVNIIWYSHYKVSGSVYTNAYRGPMETGK
                     IALCSAFIFLVLFAKNEMRTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALS
                     ASHATTAGYIILFPSLLASILILKSDFRHKTTLYTINFMLSLCAVIVTETRAAILVFP
                     FFALILIVMDSYINKRINYKLYCFIAIALLAGVFSFKDTLLMRMNDLNNDLVNYSHDN
                     TRTSVGARLAMYEVGLKTYSPIGQSLEKRAEKIHELEEKEPRLSGALPFVDSHLHNDL
                     IDTLSTRGIPGVVLTILAFSAILIYALRTAKEPYILILLFSLLVVGLSDVILFSKPVP
                     TAVFVTIILLCAYFKAQSDQYLLDK"
     gene            complement(22579..23559)
                     /gene="rfaC"
                     /locus_tag="JQH65_000696"
     CDS             complement(22579..23559)
                     /gene="rfaC"
                     /locus_tag="JQH65_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HAY6138199.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFDYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEENAENAAMI"
     gene            complement(23563..24609)
                     /gene="rfaF"
                     /locus_tag="JQH65_000697"
     CDS             complement(23563..24609)
                     /gene="rfaF"
                     /locus_tag="JQH65_000697"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HAY6138200.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNTLLLQEEA"
     gene            complement(24619..25551)
                     /gene="rfaD"
                     /locus_tag="JQH65_000698"
     CDS             complement(24619..25551)
                     /gene="rfaD"
                     /locus_tag="JQH65_000698"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HAY6138201.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            25765..26961
                     /gene="kbl"
                     /locus_tag="JQH65_000699"
     CDS             25765..26961
                     /gene="kbl"
                     /locus_tag="JQH65_000699"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAY6138202.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            26971..27996
                     /gene="tdh"
                     /locus_tag="JQH65_000700"
     CDS             26971..27996
                     /gene="tdh"
                     /locus_tag="JQH65_000700"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAY6138203.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            28235..29269
                     /gene="waaH"
                     /locus_tag="JQH65_000701"
                     /pseudo
     CDS             28235..29269
                     /gene="waaH"
                     /locus_tag="JQH65_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
     gene            complement(29256..30215)
                     /locus_tag="JQH65_000702"
     CDS             complement(29256..30215)
                     /locus_tag="JQH65_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HAY6138204.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPLTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(30219..31502)
                     /gene="envC"
                     /locus_tag="JQH65_000703"
     CDS             complement(30219..31502)
                     /gene="envC"
                     /locus_tag="JQH65_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HAY6138205.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVFLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQCGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            complement(31512..33056)
                     /gene="gpmM"
                     /locus_tag="JQH65_000704"
                     /gene_synonym="pgmI"
     CDS             complement(31512..33056)
                     /gene="gpmM"
                     /locus_tag="JQH65_000704"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HAY6138206.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            33301..33732
                     /locus_tag="JQH65_000705"
     CDS             33301..33732
                     /locus_tag="JQH65_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAY6138207.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            33874..34125
                     /gene="grxC"
                     /locus_tag="JQH65_000706"
     CDS             33874..34125
                     /gene="grxC"
                     /locus_tag="JQH65_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HAY6138208.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            34188..34655
                     /gene="secB"
                     /locus_tag="JQH65_000707"
     CDS             34188..34655
                     /gene="secB"
                     /locus_tag="JQH65_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HAY6138209.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            34655..35674
                     /gene="gpsA"
                     /locus_tag="JQH65_000708"
     CDS             34655..35674
                     /gene="gpsA"
                     /locus_tag="JQH65_000708"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAY6138210.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGAEPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            35754..36575
                     /gene="cysE"
                     /locus_tag="JQH65_000709"
     CDS             35754..36575
                     /gene="cysE"
                     /locus_tag="JQH65_000709"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAY6138211.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(36628..37101)
                     /gene="trmL"
                     /locus_tag="JQH65_000710"
     CDS             complement(36628..37101)
                     /gene="trmL"
                     /locus_tag="JQH65_000710"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAY6138212.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
BASE COUNT         9225 a         8864 c         9273 g         9871 t
ORIGIN      
        1 acgcatggtt tacctccaga cgtttagcga gccagcgttg cgacaggcgg gtgagttctc
       61 ctttgcgctg ctcatattcc atgcccatat caattaacgt gatattggtg ctttcgagat
      121 aggcgaggtg ttcaagctgt gctgcgttca tgctgtcccg tggttcgcca gtaatgccat
      181 ttgcctgcgc ccactgcttc gccgtcatgc cacccagcac gatacgggcg atcatgttgc
      241 tctcgttgct gtagtggcgc tgctgcgtcg ttttgcccat ctcggcacgg gccatattca
      301 gcgcatcgca cattggctta aagttgtttg cggcactgag acgggctttt agctggcgac
      361 gatagcgggc ggcgatttca ggcacgctac gctgtaattc ttcctcgcac tggatgaaat
      421 aacggcggat agcgcggccc tgctcgttgc gttcaaccat tgccacttct ttagccatat
      481 ccagcgatag caggtaatca tgctctattt gctggcgagt ttttgcgctc gcccgttttg
      541 gcgtgctcaa attttcaaca cggataaaat cggttccggc aataaaaccg tactggtcaa
      601 ttcggccttt aatccagtta gtaaagtcgc ggcccacccc cagcgcttta tgcaacgcct
      661 tagcgctcac aatattggct tcacgcccgc caatgcgtcc ggcaataacg ggaacaatag
      721 cggcaaagtc gttgccgtta attactcccg tgttaacgtt gggttgcggg gcggcccccg
      781 aactgaatcg gcttttttcg atagtcatat tttcggctcc gttatgcggc ggtgagattg
      841 tccgggtata gattgagaat gtcggcaatt tcaggctgag aaagtccctg atagccgcca
      901 gcgtcggcgt tatggttcac aagccgaata acttttagaa cgtcaccgcg cccggtaaag
      961 cgatagcgta aatgtgaccc tatcccgtcg gggttctttt cgtctatacg ctcaagctga
     1021 atatcaagcc tgcgctctaa ctcgctggcg tagttccttc cagacgaaag gcggcaataa
     1081 cgcagaatgt cattttctgt ccatccctct ataccagtac gcagcatata gactctggca
     1141 cgatgctttt tcggtgtgcg ctttggtgct tgaagggatt ttgtaagggg cgtaacatca
     1201 gatacgcgaa tatctgaatt agccgcctgt aatgggcggt ttttcttttc catcaggcca
     1261 ccttcccgcg gcgttctgcc agccagttgt gaatctcaac cgcatcaaac gcagttattt
     1321 tttctcccag ctttaccgga cgtggtagtg aaccgtttct tactttacgg tcgatggtgg
     1381 atacactaac gcccagcaat tcagccaggc ggaagcgtct gatatagcct tttgtaggga
     1441 tagtgtcgat gttcatgatt cccgtaacct cttagcgata gttgttgctt cgttacgggt
     1501 atttaatggc gtgcacgctc aaaaaggaat gatcgtattt tttactatcg tgatagtaaa
     1561 atttactatc accttttttt ccctgccgtg gtgcgttctt ccggtgttct gtctgcgtcg
     1621 gctatgagtt tcttcaggta atctatggat ggctcatcat agccgccccc caaaagcgcc
     1681 cagtggttta ttgtggcgtt cgcccatgct tcgttggttt ccttcccgtt cttcccttta
     1741 cactcttctg ggtagtgccg cttcacataa atcaacgcca agaggcaaat ttcgcgcttt
     1801 tgcctgtggc gctctctgtt tccgtgctca acgtttttaa catcctcata aagagcttgt
     1861 aagtttatgc gagtttcggg gctgtatatg ggctggtttt tgtccatgca ttcgccgata
     1921 tattcaaccc acggccttgt tatgtacaaa tcctctacgg atatttcgtg gaaggcatgt
     1981 tttgtataat cgagaaaaaa ataataattc gcacttacgt ctagttctct ttttctgtgt
     2041 gcggggctga atatgcgtgc gggagtgtca atagaatcaa gccctgcgat gtcgagtata
     2101 taggaggcgt taacatgcca tagcccaagc acatggcaat ctatatgatt tacaacgcct
     2161 gccttaaagc ttttttcgtc ctctacttca gtgattatgt cggcaagtaa taagcagtct
     2221 tctttgacgt ctgggaataa agagcaattg cggtaaagac tcgcgatgct atgtaatgtg
     2281 ccgtcgtagc cgcaaaggta cgcgctcaaa tatatagatt ctgccgccgc cataatcaaa
     2341 agatcatcaa tatctatttc taataattcg gctgctctgt taagcctgaa aaaaggggcg
     2401 ggtggtaatt ttttcattgt gtcacctttt acgctgcgcc ttattttgtg attgagccag
     2461 gcggtaaggc ttcccgcttt tcgccccgtc gagctaggct caatctattc agttagttcg
     2521 cccttctgag aggcgttacg ttgtagtctt ttccgctttc cagtgccacc agcagatcag
     2581 cccactgtgc cagtgctgct ttacgctcat caaaatactg atggcggtta taaatcccct
     2641 ctacgccctt aatgcgatga ttgaggcatc gttccgccac aacaggatca acacccaacg
     2701 ctgctaaatg agtacgtgcg gtgcgtcgaa agtcgtgaat cgtgaaatta ggcacatccg
     2761 gcatttcggc gcgaacctta gccagtgcta cgggtaaagt gctttcctga atatgtggga
     2821 tcattctgtt ttgcatcttc ctggccggaa gcacccatgc gctattacat gaaaaggtgt
     2881 gtagctctct caaccattca acggctgggg aaggtagagg tatatcaata gggtctccgt
     2941 ttttgctgcg ttcttccggc aaatgccata ccgcaccatc taaatcaaac tcttcccatc
     3001 gtgcggcgca taattccatt ttgcggacgc ataacgctaa cagtaatttg aacgtgattt
     3061 cgttctggcg actgaatccc ttagccgtgc gcatggcttt gaatagctgg attaactcat
     3121 cgcgggttaa ccagcggtca cgggcagctt ctttccctcc ggcgtctgcc acctcaaagg
     3181 ctgaacaggg gttaatctct agcgcgtgcc gtttgattcc gtagtcgaat atgcggcgcg
     3241 tccatctcag cacgtccgtt gctatggtcg gcgctccacg gtcaacaata cctttcagca
     3301 tgtcatcgat atggcgcggt ttcacgtcct ctaccttcat gctgccaatg caggggttta
     3361 tatctttgtc gatacggcgg cggagtatat cggggtgctt ccagcgcggg aggatctgac
     3421 gctcaaagta ttcagcggca agctctgaaa cgcgcatagc gttcttctct gcttccatct
     3481 tcgccagtgc ttcggttttg cgcttctgct tctctcctgc aacgtcatag cccagcgcaa
     3541 cgcgagccga tagctctttg gctgtctctc tggcctttga tagtgatagc tcgctatacg
     3601 aaccaattag catggcgcgg gacttccctg cgagtttata gcggaatctc catgtggggg
     3661 ttttgtcggc ttcacggtaa cgtaggtata aaccgttacc gtctgcccgc ccctcgaatc
     3721 gctctccgct cttaatccat gcgcggatct gcatgtctgt tagttttggc attttgcgaa
     3781 tatccaaact ttgaataggg ggtgtaccca aagcaatgta cccaaacctc attgggtaca
     3841 cctttgtgta caccataatc atgcgctttg ttgcgtcttt atgatagccc atgataacaa
     3901 aaaagcccgc tgggttgcgg gcttggtgtg atttatgcgt tgtcctaaat gttgctgata
     3961 gcttactcga tattctgaat ctgctcgcgc atctgctcaa tcaacacttt cagctcaatc
     4021 gcggagtttg tcacttcggc attgatagac ttcgacgcaa gagtgttcga ctcgcggttg
     4081 aattcctgca tcataaagtc cagacgacga ccaacggctt cttttttctt cagaatgttg
     4141 taggtctctt tgacatgcgc ttccaggcgg tccagttctt cggcaacgtc aattcgttgt
     4201 gccagcagaa ccagctcctg ctccagacgg ttgttttcca actgaacctg agcatcttcc
     4261 agcttcgcga ccagacgctc acgctgccat tgcaggattt ccggcatatg ggcgcggact
     4321 ttgaccactt cggcggtgac gccttccaga cgctgctcga tcaatgcttt cagtgcctga
     4381 ccttcggttt cgcgcgcgac aataaagtcg tccagcgtac catccagcgc cgcgagaatt
     4441 tcagcggcaa tggcgtcaag atcctgctcc tgggctgcca tcacgcccgg ccagcgtaga
     4501 atatcaaccg ggttgatttc cccttcgtca ctctgcattt ttacccagtt cgcggcagtt
     4561 accagctgtt tagccagttt ttcgttgagg atcagctcac cttgcgcgct aatatctggc
     4621 tcatagcgca gggtacattc cactttaccg cgcgtcaggc gagaacgaat acgctcgcga
     4681 acgacaggtt caaggctacg gaactgctcc ggcagacgaa agtaagtttc cagataacgc
     4741 tggtttaccg agcgcatttc ccaggttgcg ctcccccatt cacccttgat ttcacgccgg
     4801 gcgtaggcgg tcatactgcg gatcatagac gttcctgttt ataaaaggag aggtggaagg
     4861 attatagcca tcgatgcctt gtcaggatag gaataaccgc cggaagtccg tataatgcgc
     4921 agccacattt gtttcaagcc ggagatttca atatgcgtcc agcaggccgt agcaataatc
     4981 aggtgcgtcc cgttaccctg actcgtaact atacaaaaca tgcagaaggc tcggtgctgg
     5041 tcgaatttgg cgataccaaa gtgttgtgta ccgcctctat tgaagaaggc gtgccgcgct
     5101 tcctgaaagg tcagggccag ggctggatca ccgcagagta cggcatgctg ccacgttcta
     5161 cccacacccg taacgctcgt gaagcggcga aaggtaagca gggtggacgc acaatggaaa
     5221 tccagcgtct gatcgcccgt gctcttcgcg cggcagtaga tttgaaagcg ctgggtgagt
     5281 tcaccattac gctggactgc gacgtgcttc aggctgatgg tggcacgcgt accgcgtcga
     5341 ttacgggtgc ctgcgtggcg ctggcagatg cgctacagaa gctggtggaa aacggcaagc
     5401 tgaaaaccaa cccgatgaaa gggatggtag ccgcagtttc tgtcggaatt gtgaacggcg
     5461 aagcggtttg cgatctggaa tacgttgaag actctgccgc agagaccgac atgaacgtag
     5521 tgatgaccga agacgggcgc atcattgaag tgcaggggac ggcagaaggc gagccgttca
     5581 cccatgaaga gctactcacc ttgttggctc tggcccgagg gggaatcgaa tccattgtag
     5641 cgacgcagaa ggcggcgctg gcaaactgat ttttaaggcg actgatgagt cgcctttttt
     5701 ttgtctgtag aaaagtaaga tgaggagcga aggcatgaaa ccatatcagc gccagtttat
     5761 tgaatttgcg cttggcaagc aggtgttaaa gtttggcgag tttacgttga agtccgggcg
     5821 caaaagcccc tatttcttca acgccgggct gtttaatacc gggcgcgatc tggcactgtt
     5881 aggccgtttt tacgctgaag cgttggtgga ttccggcctt gagttcgatc tgctgtttgg
     5941 ccctgcttac aaagggatcc cgattgccac cactaccgcc gtggcgctgg cggagcatca
     6001 tgaccttgac ctgccgtact gctttaaccg caaagaggca aaagaccacg gtgaaggcgg
     6061 caatctggtt ggtagcgcgt tacaaggacg cgtaatgctg gtagatgatg tgatcaccgc
     6121 cggaacggcg attcgcgaat cgatggagat tattcaggct aatggcgcga cgcttgctgg
     6181 cgtgttgatt tcgcttgatc gtcaggaacg ggggcgcggc gagatttcgg cgattcagga
     6241 agttgagcgt gattacaact gcaaagtgat ctctatcatc accctgaaag acctgattgc
     6301 ttacctggaa gagaagccgg aaatggcgga acatctggcg gcggttaagg cctatcgcga
     6361 agagtttggc gtttaaagaa actcgccgga tgaaaagtca tccggcgtca tattactgca
     6421 actgtgccgc aattagcggc cagcgggcgt caaaatcatc cgtcgggcgg tatttaaatt
     6481 cgctgcggac aaaacgtgac agcatacctt cacagaaggc caggatctgg cttgccagca
     6541 gggtttcatc ggtggtgtaa ccttcaccct cacgcattct cttttcacgc aatacctgac
     6601 gcagctgcgc ttcaatacgc tcgaacagct ggttgatgcg cccttgcagg cgatcctgtt
     6661 caaacattag cgcatgacca gtgaggatgc gggtcaggcc aggattacgc tcaccaaaac
     6721 cgagaagcag caacacaatc agacgcaggc gcgctgtggt gtctttctca tctttcagaa
     6781 tcaggttgat gcgagtaatc aggctatctt cgataaactc aatcaggcta tcgaacatgc
     6841 gggtcttact ggggaagtgg cgatacagtg ccgcttcgga aacgccgaca gaggcggcca
     6901 gttttgccgt cgtgatacgt tggcttccat cgctggattc cagcatcagc gccagagact
     6961 gaagtatttc ctcgcgacgg ttccttttcg cagtttgttt ttctgccatg ttacaaaata
     7021 cccctgaaaa taagcacttg ccaggcggca cccacgctat gaccgcaaac gaaatgtttg
     7081 cggctatgtt atgacgttat tcggatgcgt atgtgttact gacgaccaga gtgaccaaag
     7141 ccgccttcac cgcggtcggt ggcgtcgaaa tcttccacca gattaaattc agcctgtact
     7201 accggaacaa aaatcatctg ggcgatgcgt tcgccaggtt gaatggtgaa gctgtcctga
     7261 ccacggttcc acacggaaat catcaactgg ccctgatagt cggaatcgat caatcctacc
     7321 aggttaccaa gcacgatacc gtgcttatgt cccaatccgg agcgcggcag catcattgcc
     7381 gccagtgaag gatcggcaat atgaatcgcc agcccggtcg gaaccagcgt agtgtcaccc
     7441 ggagccagtt ctacggcgtc gtcgagacag gcacgcaggt caagtccggc agagccagag
     7501 gtggcataag tcgggagcgg aaattccttc ccaacgcgcg ggtccagaat cttaacgtcg
     7561 atttttttca tcataacggg tcacgatctc gtcgagtaat agttggccaa ggagctcttt
     7621 gcgctcaagc ggtaagactt tatctccgtc ctgccagaaa aggtgtaatg cgttgttttc
     7681 gctgttaaat ccttgagttg gctgggaaac atcgttcgcg cagatcagat caaggttttt
     7741 acggatacgt ttttgccggg cgtattcttc cacattattt gtttcggcgg caaatccaac
     7801 gacgtagggt cgatggtctt ttagtgcggc aacgcctgcg acgatatcgg ggtttttaac
     7861 catttttatt gttaattcat caccctgcgt ggcctgcttt ttgatttttt ctggggccac
     7921 ggtagctgcg cgataatccg ccacggcggc gcagccgata aaaatatttt gctgctgtac
     7981 agaagcattc acggcggctt ccatttccag cgcggtcatc acatcaacac gtttaacaaa
     8041 cggtggcgtc ggtagtgaaa ccggacctga taccagcgtg acgttcgcgc cacgacgggc
     8101 agcggcggcg gcgatagcaa aacccatctt gccggagctg tgattagaga tataacgcac
     8161 gggatcgagc ggttcacgcg tcggaccggc ggtaatcata atgttcagat gtttcaggtc
     8221 gttgacgggc gaaaaatgcg ccaccgccat atccacaatg gttaacggat cgagcattcg
     8281 cccaggaccg atatcaccac aagcctgact gccactgtct ggcccccaga tgagcaaacc
     8341 acgggaagca agcccctcta aattatgctg cgtggcagcg gcacggtaca tctgctggtt
     8401 catggcgggg agcacggcta caggcgcagg tgtagccaga caaatcgtcg ataccaggtc
     8461 attcgccatt ccggcagcaa cacgggcaat caaatctgcc gtggcagggg cgagaatcac
     8521 taaatcagcc catttaccca gctcaatatg gcccatagcg gcttcggctg ccgggtccag
     8581 caaactgtcg gaaacgggat aaccagaaac cgcctgcaag ctaagtgggg tgataaaggc
     8641 ttttgccgct tcggtcatgg ctacgcggac gtcggccccg cgatcgcgca aacgacgcac
     8701 cagttcaggg gttttatagg cagcaatacc gccgctaacg ccgagaacga tttttttacc
     8761 ggccaggctc atgatgattt ttcctgttgg gtgacaccag aagttggcga ttttatcaca
     8821 atacttttgt tgtcgtgcct tcacctgaga atcactttgc gaggcgcttt ccaggattga
     8881 aaactggccg tcgatttaac ggaacggcta tgacaggatg cgagcaccac aaaggaggtg
     8941 aaggtgaaaa acaattcaca gctgttgatg ccgcgcgaaa aaatgctgaa gtttggtatt
     9001 agcgccttaa cggatgtcga gctgctggcg ctatttctgc gtaccggaac gcgcggtaaa
     9061 gatgtattaa ccctggcaaa agagatgctg gagaatttcg gctctcttta tggcttgtta
     9121 acctctgaat atgaacaatt tagtggcgtt catggaattg gcgtggcgaa atttgcccag
     9181 ttaaagggga ttgctgaact ggcgcggcgt tactacaacg tgcggatgcg tgaagaaagc
     9241 cctttactca gcccggagat gacgcgggaa tttttacaaa gccagcttac gggtgaggag
     9301 cgggagatct ttatggtgat ctttctcgac tcacaacacc gggttataac gcatagccgt
     9361 cttttttccg gcacgctaaa ccatgttgaa gtccatcctc gggaaattat ccgcgaagcg
     9421 ataaaaataa acgcctcggc gctgatcctt gcacataatc acccttcggg ttgtgctgaa
     9481 cccagtaaag cggataaact cattactgaa cggataataa agagttgtca gttcatggat
     9541 ttacgcgtgc tcgaccatat cgtgattggg cgtggagagt atgtttcttt tgccgaacgc
     9601 ggctggattt aacccgctat gcgcgatcct tcgggatctt tgtctgttcg ggacttgagc
     9661 acatcgctga gtcagcgtat actacgccac ctttgagaat ctcgggtttg gcatttgggc
     9721 ctggcaatcg agagttcaca tagaactgcg atgactgggc tgtaaagcct gacgaggcgc
     9781 caatacccca tacgaagctc gagctaattt gatttttgga gaatagacat gtcccgagtc
     9841 tgccaagtta ctggcaagcg tccggtgacc ggtaacaacc gttcccacgc actgaacgcg
     9901 actaaacgcc gtttcctgcc gaacctgcac tctcaccgtt tctgggttga gagcgagaag
     9961 cgttttgtca ccctgcgcgt atctgctaaa ggtatgcgtg taatcgataa aaaaggcatc
    10021 gatacagttc tggctgaact gcgtgcccgt agcgaaaagt actaagtact tagaggaaat
    10081 aaatcatggc taaaggtatt cgtgagaaaa tcaagctggt ttcttctgct ggtactggtc
    10141 acttctatac cactacgaag aacaaacgta ctaagccgga aaaactggaa ctgaaaaaat
    10201 tcgatccagt tgttcgccag cacgtgatct acaaagaagc gaaaatcaaa taattctcgc
    10261 tttgatgtaa caaaaaacct cgctccggcg gggttttttg ttatctgctt gcccccatat
    10321 tgactgcatc tgttcattcc tggagatgct atgcctgaat tacccgaagt tgaaaccagc
    10381 cgccgcggca tagaaccgca tctcgttggt gcaaccattc ttcatgcagt ggtgcgcaac
    10441 ggacgcttgc gctggccggt ttcagaagag atctaccgtt taagcgacca accagtgctt
    10501 agcgtgcagc ggcgggctaa atatctgctg ctggagctgc ctgagggctg gattatcatt
    10561 catttaggga tgtctggcag cctgcgcatc cttccagaag aacttccccc tgaaaagcat
    10621 gaccatgtgg atttggtgat gagcaacggc aaagtgctgc gctacaccga tccgcgccgc
    10681 tttggtgcct ggctgtggac caaagagctg gaagggcata atgtgctggc ccatcttgga
    10741 ccggagccgc ttagcgacga tttcaatggt gagtatctgc atcagaagtg cgcgaagaaa
    10801 aaaacggcga ttaaaccgtg gctgatggat aacaagctgg tggtaggggt agggaatatc
    10861 tatgccagcg aatcactgtt tgcggcgggg atccatccgg atcggctggc gtcatcactg
    10921 tcgctggcag agtgtgaatt gttagctcgg gtgattaaag cggtgttgct gcgttcgatt
    10981 gagcagggtg gtacaacgct gaaagatttt ctgcaaagtg atggtaaacc gggctatttc
    11041 gctcaggaat tgcaggttta cgggcgaaaa ggtgagccgt gtcgggtgtg cggtacgccg
    11101 attgtggcga ctaaacatgc gcagcgggca acgttttatt gtcggcagtg ccagaagtaa
    11161 ttcatgcgcg ccggatcgca taccatccgg cataaacgct acgctaactt cgccatcagc
    11221 gcctgatgga cattctccgg caggaaatgg gtgacatcgc cctgatggcg cgccacctct
    11281 ttcaccaacg atgaagagat aaacgaccac tctttcgacg gcatcagaaa cacactttcc
    11341 agttccggca ttaagtggcg attcatatgc gccagctgca tttcatattc aaaatccgcc
    11401 accgcacgca ggccacgaat cagcaccgta gcgtgttgat tacgggcgaa gttcgccatc
    11461 aaatcactaa acccaactac ttccacgttc cccagatgcg cggttgcctg ctgtgccagc
    11521 gccacacgct cttccagggt aaacatcggt tttttactgg ggctggcggc aatcgccaga
    11581 ataacgtgat cgaacatttg cgtggcgcgc gtcacgatat cgatatgacc attggtaatg
    11641 ggatcgaaag tacccggata aatcgcccgt ttttgcataa caacctcaat gcgttttcgg
    11701 tggcaggtaa ggttccagca gttgaagcag gcgctgtagc gcgccctggt tttgatacag
    11761 tacttcaacg gcatgacggc catagaaact acggtaatcg gcgtcggtga gtaaagagga
    11821 aacctctttt gcaagcgtag tggcatcggt aacggtaatc agcccgcttg cctgctccag
    11881 ccgcgcgcaa atgtctttaa aattaaaagt atgcggcccc atcaataccg gaatagcgtg
    11941 tgcggcagct tccagcggat tatgcccacc acgttcaacc agtgaaccgc caacaaaggc
    12001 gagatcggca atgccgtaca gtaacatcaa ctcgcccatc gtatcgccaa ccacaacctg
    12061 cgtgctggtg gaggggactt cccctgaaga gcgtgtgata tagcttagtc cagcctggcg
    12121 gacaaggtta atcgcatccg ggaagcgttc tggatgacgg ggtaccagga tgagcaataa
    12181 attcgggaat tgctgtaaca atacctgatg tgcggcgatc accacactct cttcaccttc
    12241 gtgagtgctg gtggcaatcc ataccgggcg gtgtggtgcc cactggcggc gcagcgtcac
    12301 ggctttagca gccaactgcg gcgttacaga aatatcgaat ttcaggctac cggtaacggt
    12361 tacctggtta ttttttgcgc ccagcgccac aaaacgtgca ccatcttctt cattttgggc
    12421 ggcaatcagc gtaatacgac gcagcaagcg acggacgaat ttacccagtt tggcataacc
    12481 tgcggccgag cgggcagaga gtcgcgcgtt cgcgatcacc agcggaattt tacgtttatg
    12541 tagcgccgca atcaggttag gccatagttc ggtttccata atcaacacca gtttagggtc
    12601 gactttattc aggaaacgat taagtgcatc gggcaggtca tacggcagat aaacgtgctg
    12661 aacatccttc ccgaaagccg attgtacgcg ctccgaaccg gttggcgtca tggttgtaac
    12721 ggtaatcggt aaatcaggat aacgatgacg cagcgcgcgc accaacggga tcgccgccag
    12781 agtttcaccg acggagacgg agtgcagcat aatgccgcct ggttttagcg gatggcggta
    12841 aaaaccgtaa cgttcacccc agcgttttcg ataggccgga gccttacgtc cgcgcaccca
    12901 gagccgtatc cagatcagcg gctgaataag gtagagaagg gcggtgtaaa gcaattcgag
    12961 catagtaaat agctgactta tggatgtgct ggggattcta tgtatttagc tgtggcttta
    13021 ccattacttt tcccgttttt gacttaaata gcaatttacc cgccaattct tgctgaacat
    13081 ctatactttt tttgtatttt tatgcgattc attgatttct gctgtacgtg caggaaggaa
    13141 tcgcagagca acagtgaaag aatatggttg ctaatgattt tatttgattg atttctttat
    13201 tgaaaatcaa caatgtgctt aaaaatcatt cgggtcggat tgccgcgaaa gaagtgatac
    13261 actagcgcgt caatatcagt gcggtatcag tattcaggta gctgttgagc ctggggcggt
    13321 agcgtgcttt ttctgcttca ccttaatcgg ataatctcaa caaaaagagt tacttgtgga
    13381 taagctattt cgaagaattt tgctcattaa gatgcgtttt catggggata tgttattaac
    13441 tactcccgtc attagttcgc tgaaaaaaaa ttaccctgac gcaaaaatcg atgtgctgct
    13501 ttatcaggac accatcccga tcctgtctga aaatccagag attaacgcgc tctacggcat
    13561 aaaaaataaa aaagcaaaag cctcagaaaa aattgccaac ttttttcatc tcatcaaggt
    13621 attacgtgcc aataagtatg accttatcgt caatctcacc gatcaatgga tggttgctat
    13681 actggttcgc ttattaaatg cccgtgtgaa aatttcccag gattatcatc atcggcagtc
    13741 tgctttttgg cgtaaaagtt tcacccattt ggtgccgttg cagggtggaa atgtggtgga
    13801 aagtaactta tccgtgctga ccccattgcg agttgattcg ttggtgaagc agacaaccat
    13861 gagttacccg cctgcaagct ggaaacgtat gcgtcgcgaa cttgatcatg ctggtgttgg
    13921 acaaaattat gtggttatcc aacctacggc gcggcaaatc ttcaaatgct gggacaacgc
    13981 caagttttcc gctgtgattg atgccttaca tgctcgtggt tatgaagttg ttctgacgtc
    14041 cggcccagat aaagacgatc tggcctgcgt caatgaaatt gcgcagggat gccagacgcc
    14101 accagtaacg gcgctggctg gaaaggtgac cttcccggaa cttggtgcgt taatcgatca
    14161 tgcgcagctg tttattggcg ttgattccgc accggcgcat attgccgctg cagttaatac
    14221 gccgctgata tcgctgtttg gtgcgacaga ccatattttc tggcgtccct ggtcaaataa
    14281 catgattcaa ttctgggcgg gagattaccg ggaaatgcca acgcgcgatc agcgtgaccg
    14341 aaatgagatg tatctttcgg ttattccggc ggcagatgtc attgctgctg tcgataaatt
    14401 actgccctcc tccacgacag gtacgtcgtt atgatcgttg ctttttgttt atataaatat
    14461 tttccctttg gcggtttgca gcgcgatttt atgcgtattg ctcagacagt cgccgcccga
    14521 ggtcatcatg ttcgggttta tacccagtcg tgggaaggcg aataccctga tgtatttgaa
    14581 ctgatcaaag tgccggttaa atcgcatacc aatcacgggc gcaatgcgga gtattttgcc
    14641 tgggtgcaaa aacatttacg cgaacatccc gtcgataaag tcgttggatt caacaaaatg
    14701 ccggggctgg acgtttatta tgccgctgat gtttgttatg ccgagaaagt cgcgcaggaa
    14761 aaaggctttt tctatcacct gacgtcacgt tatcgccatt atgccgcctt tgagcgggca
    14821 accttcgaac agggcaagcc gacacagctg ctgatgctga cagataagca aatcgccgat
    14881 ttccagaaac attatcagac tgaagcggag cgttttcata ttctgccacc ggggatttat
    14941 cctgatcgta aatatagcca gcagccagct aatagccgtg aaatcttccg taagaagaat
    15001 ggaataaccg aacaacaata tttattgttg caggtcggtt cagacttcac gcgtaaaggt
    15061 gtcgatcgtt ccattgaagc acttgcttcg ttaccggatt cgctgcgcca caacacattg
    15121 ctatatgttg ttggacagga taaaccgcga aaatttgagg cgctggcaga aaaacgcggc
    15181 gtgcgcagta atgttcactt cttctcgggg cgcaacgatg tctcggaatt aatggcggcg
    15241 gcggatttat tactgcatcc tgcctaccag gaagcagcgg gaattgtgct actggaagcg
    15301 attactgcag gattaccggt actaacaaca gccgtttgtg gctatgcgca ttatattgtc
    15361 gacgccaatt gcggcgaggc tattgctgag ccattccgcc aggaaacatt gaatgagatt
    15421 ttacgcaaag cgttaacgca atcttcattg cgccaggcct gggcggaaaa tgcgcgacat
    15481 tatgctgata cacaagattt atacagtctg ccagagaaag cggcggatat cataacgggt
    15541 ggtctggatg gttgaactta aagagccgtt tgccacgtta tggcgcggta aagatccttt
    15601 tgaggaagtt aaaaccttgc agggtgaggt ttttcgtgaa ctggaaactc gccgcactct
    15661 gcgatttgaa atggcgggca aaagctattt tctcaaatgg catcgcggca cgaccctgaa
    15721 agagataatc aaaaatttac tctcattgcg gatgccagta ttaggcgcag accgcgaatg
    15781 gagtgcgatt catcgactgc gggatgtcgg cgttgatact atgtatgggg tggcatttgg
    15841 cgaaaaaggt attaatccgc tgagcagaac ctcgtttatt attaccgaag atctgacccc
    15901 aaccatcagt ctggaagatt actgtgctga ctgggcgacc aacccaccag atgttcgcgt
    15961 aaagcgtatg cttattaagc gtgtcgcgac gatggtgcgc gatatgcatg ctgcgggcat
    16021 taaccaccgt gactgttata tctgtcattt cctgctgcac ttgccttttt ccggtaagga
    16081 agaggagtta aaaatttcgg taattgacct acaccgggcg cagcttcgca cgcgcgttcc
    16141 acgtcgttgg cgcgataaag atcttatagg gctttatttt tcttcaatga atattggcct
    16201 gactcagcgg gatatctggc ggtttatgaa agtgtatttt gccgccccgc ttaaagacat
    16261 tctcaagcag gaacaaggac tgctgtcgca agcagaagca aaagccacaa aaatcaggga
    16321 aagaacgatt cgaaaatcgt tgtaatttcc ggagagaaat atgagtgccc actattttaa
    16381 tccacaagag atgatcaata agacaatcat cttcgatgaa aggccagcgg cgtcagtggc
    16441 atcatcattc catgttgctt atggcattga taaaaacttt ctttttggtt gtggtgtttc
    16501 aatcacgtca gttttgttac ataacaacga cgtgagtttt gttttccacg tttttattga
    16561 tgatatccct gaagccgata tccagcgttt agcccaattg gcgaaaagct atcgtacctg
    16621 tatccagatc catctagtaa attgtgaacg gcttaaggca ttaccgacga ccaaaaattg
    16681 gtctattgcc atgtatttcc gttttgtaat tgcagattac tttattgatc aacaagataa
    16741 gatcctttac ctggatgctg atatcgcctg tcagggaaac ttaaagccgc tgataacaat
    16801 ggatcttgcc aataacgttg ctgctgttgt tactgaacgc gatgctaact ggtggtcgtt
    16861 acggggtcaa agtctgcagt gtaatgaact tgaaaagggc tactttaatt caggtgtcct
    16921 gttaattaat acactagcgt gggcgcagga gtccgtttct gctaaagcga tgtcgatgct
    16981 tgctgataaa gccatcgttt cccgtttcac ctatatggat caagatatcc ttaatcttat
    17041 cctgttaggg aaagttaaat tcattgatgc taaatacaat acgcaattta gtttaaatta
    17101 tgaattaaaa aaatcatttg tttgtccaat taatgatgaa accgtattaa ttcattatgt
    17161 cggcccgaca aaaccctggc attactgggc cggttatcca agtgcgcaac cttttatcaa
    17221 agccaaagaa gcatcgccct ggaaaaatga accgttaatg cggccagtta actcaaacta
    17281 tgctcgttat tgcgccaagc ataattttaa acaaaataaa ccaattaacg ggataatgaa
    17341 ttatatttat tatttttatt taaagataat aaaatgaaat tgagaaaagt taaatgaatg
    17401 aatttataaa agaacggttt tcgtatttag cagataataa aaaagaaaac gccccagagc
    17461 taaatgtttc ctacggtatc gataagaatt ttttgtatgg tgctggcgtt tcaatttctt
    17521 ccgttttgat taataattca gatattaatt ttgtctttca tgttttcact gattatgtgg
    17581 atgatgatta tttaaagtca tttaatgaaa cagcaaaaca atttaatacc tcaattattg
    17641 tatatttaat tgaccccaaa tactttgctg atctgccgac gtcacagttt tggtcatacg
    17701 cgacatactt cagggtattg tcttttgaat atctgagtga aagtatttcc acactgctgt
    17761 atctggatgc cgatgttgtt tgtaaaggaa gcctgaaacc tctcacagaa attatattta
    17821 aagatgagtt tgctgcggtc attcctgaca atgatagtac tcaggcggca tgtgcaaaac
    17881 gcctcaacat tcccgaaatg aatggacgtt atttcaatgc aggcgttatc tatgtcaatc
    17941 ttaaaaaatg gcatgaagca aatttgacac cgtatttact caaactttta cgaggggaaa
    18001 ctaaatatgg ctctcttaaa tatttagatc aggatgcgtt gaatatcgca tttaatatga
    18061 ataatatcta ccttgcgaag gattttgata ctatttatac cctgaaaaac gaactttatg
    18121 atcgtagtca tcgaaagtat cagcaaacca ttaccgataa aacagtgttg attcactata
    18181 cagggataac taaaccatgg catagctggg ctggatatcc gtctgcatca tactttaata
    18241 tcgcgcgtga acaatctccc tggaagaaat atcctcttaa agaggcgcgg actgttgcag
    18301 aaatgcagaa acaatataag catctgtttg cccatggtga gtatataaaa ggcataactt
    18361 cattaattaa gtacaagctt aagaaataaa ttactcttat gattacaagt atacgttatc
    18421 gcggcttctc attttattac aaagataatg ataataaata taaagaaatc tttgatgaaa
    18481 tactggccta taattttaaa acagtaaaag tattatgtaa tattgatgat accaaagtat
    18541 cgttgattga tacgaaatat ggtcgctatg tttttaaagt ctttgctccg aaaacgaaaa
    18601 ggaatgaacg ttttttaaaa tcttttgtaa aaggtgacta ctatcagaat ttgatcgttg
    18661 aaaccgatcg tgtcagaagt gcaggtctta cttttcctaa tgatttttat tttctggctg
    18721 agcgtaaaat tttcaactat gccagtgttt ttatcatgct tatcgagtat gtcgagggcg
    18781 tagagctcaa tgatatgccg attattccag aaaatgttaa agcagaaatt aaggcttcga
    18841 tggaaaaatt gcatgcctta aatatgctat cgggcgaccc acaccggggt aactttatcg
    18901 ttagtaaaga tggtgtcagg ataattgacc tatccggtaa aagctgtacg gcagagcgta
    18961 aagctcgtga tcgtctggcc atggaacgtc acctgggtat tgccaacgaa ataaaagatt
    19021 atggctacta ttcagtaatc tacagaacca aactacgcaa atttattaaa aaattaaaag
    19081 gcaaagcgta aaccacacag tcaaaacgga accaacatgg atttattagc agagagtatt
    19141 actgaagtcg ctgtctctgg ggaaattgct aacaccgatc gtgtgttaaa tatcgcttac
    19201 ggtattgacc gtaacttttt atttggtgcg gcagtatcta tgcaatcagt tgttatgcat
    19261 aacccggacc ttgcggttaa gtttcatctc tttaccgact acattgatga agattatcta
    19321 caacgtgtta atgcttttac cagcaaaaat gctaacgttg aagtaataat ttataaagtc
    19381 tccaatgcct ttattgatat cttccccagc ctgaaacagt ggtcttatgc aacattcttc
    19441 cgtttagttg cgttccagta tttgagtgaa actattgaaa atctgttata tatcgatgct
    19501 gatgtcatct gtaaaggctc attagctgga ttgcttgata ttaattttga tgaggataag
    19561 ttcgcagctg ttattaaaga tgtgcctttt atgcaggaaa aaccagcgaa gcgtctggct
    19621 atagagggac ttccagggaa ttatttcaac gccggtgtag tatatctgca gcttgaagca
    19681 tgggcgaaaa atgattttat gaataaagcc attgctatgc tggcaagtga cccgcagcac
    19741 acgaagtata aatgccttga tcaggatatt ttaaatattc tgttctttgg tcattgtaat
    19801 tttattagcg gcgattatga ttgcttttat ggcattgact atgagttaaa aaataaaagc
    19861 gatgaagatt ataaaaagac cattaccgat gatactaagc tgattcatta tgttggcgta
    19921 acgaagccct ggaacgactg gacgaattat ccctgccaga agtattttaa tgaggcttat
    19981 caggcttctt gctggaatga tgtggcgttt attccagcca cgaatgaaaa gcagtatcaa
    20041 gtgaaatatc aacatgcaaa gaaaaatggt gatacgttta acgcttttat ttacttcatt
    20101 aaatttaaat taaataagta taaaagaaaa ctatttggat aataataaaa atcatggatg
    20161 tctgagttac aataataatt aaggatgagt actatcatat tttattagtt caggcaagag
    20221 aacacgatga gcaatgatta ccctttagta tctataataa taccgacgta taattcatct
    20281 gattacatta ctgaaactct aacgaaatta gaaaaacaaa cttacccaaa ttttgaaatt
    20341 gttattgtta atgatggttc taaagataac acatcaaacg ttttgagaga gtatgggtta
    20401 acccactctc gattaattat tgtcaataaa gaaaatggcg gtgtttcgtc tgccaggaat
    20461 acaggtatcc gcaaggcgca aggacagttt atatgtttta tggatgatga tgatgagata
    20521 gatcctaact atctgctgaa gatgtatacc agacaacatg agacgggagg ggatgcgatt
    20581 tattgtgggc tttatggcca ttatataaaa gatggtgtta cttactcacc tataaataca
    20641 gagtttaatg aaggatcttt acttttcgac tttttttata aaaaggttag attccatata
    20701 gggtgcttgt ttataagaaa acaacttctg gaagagaata atcttttttt tgatgaagat
    20761 ttacgactag gagaagatct tgattttatc tatcgactgc taattacatg cgatatgtat
    20821 gcggttccat attatatgta taagcataac tatagagaaa attccttaat gaactcatgt
    20881 agaaccatca ctcattatcg acatgagtca tttgcgcacg aaagaatcta ctcttctgtg
    20941 atgcagttat acaaaggtaa ccggaaagaa gaaattcata cattattgag tcaaaataga
    21001 gcttatcata aaactcgtta tttatggaat gttctactta atggtgattt taaactattg
    21061 aatcaattag ttgaaagcaa tgaaaaagaa ttaagatatt gtaatcttcc tggcaagaga
    21121 gataagagac gagcaaaaat attagcatca aaaaattata ttctctggag gatggtaaga
    21181 ctggtaaata gaaaaaagaa taaacgttag atcttgcatt aaataaccca tcctcgtagc
    21241 ataggttgaa attatatgtc gttttgttgg aatgaaatta attctggtat aaagtcttta
    21301 attcttatat tatgtatttt ttctttaatg actttgtctt tatgggatga tgttgcaaca
    21361 aagtttcttc atgcagctgg aattatatct gcattgtatt ttcttgcgac accaaaaaaa
    21421 acaataacta ataatcctac tttgttaatt ttcatctcat tatgtctttt gggtatcgta
    21481 aatatcatct ggtattcaca ttataaagtt tcaggctctg tttataccaa tgcatatcgt
    21541 ggcccaatgg aaactggaaa aattgccttg tgtagcgctt ttattttctt agttcttttt
    21601 gctaaaaatg agatgagaac aaaaataaaa tttgggaaac taattctgtt cgcatccctg
    21661 gcaacgcagt tacttttttt tgcgcatgcc atgtggcaac atttctattt aaacgtcgac
    21721 cgtgttgcat tatcagcttc ccacgctaca acagcaggct acatcatcct ttttccttct
    21781 ttactggcat caattctcat tttaaaatcc gactttagac ataaaacaac attatataca
    21841 attaacttca tgcttagctt atgtgctgtc atagtaactg agacgcgtgc agccatatta
    21901 gtgtttccat tctttgcgtt aatattaatc gtaatggata gttatattaa taagcgaatt
    21961 aattataagt tatattgttt tattgcgatt gcattattag caggtgtatt ttcttttaaa
    22021 gatacattgc ttatgagaat gaatgactta aataacgatt tagttaatta ttcgcatgat
    22081 aacaccagaa cttcagtcgg tgcccgtctg gcaatgtatg aagttggctt aaaaacatat
    22141 tctccaatag gacaatcact ggaaaaacgt gcagaaaaaa tacatgagct agaagaaaaa
    22201 gagcctagat tgagtggcgc tttacctttt gtagattctc atttgcataa cgatctcata
    22261 gatacgttat caacgcgtgg tattcctgga gttgtattaa caattttagc attttcagca
    22321 atactcatat atgccttaag aactgctaaa gaaccttata ttttaatctt gcttttttca
    22381 ctactggtag taggcctaag tgatgtaata ctcttttcta aaccggttcc gactgctgtg
    22441 tttgtcacca taatattgct ttgtgcttat tttaaagcac aatcagacca atatttatta
    22501 gataagtaat gaatatatga aataaaatca gcaaggaggc tgattttatt tcaaaattat
    22561 ctattaaggg gttgtaattt aaatcatagc agcgttttca gcgttttctt caatgaatcg
    22621 cttaacagca tttgctgtca attgagacaa ctcattcccc ggagccctac aaaccatttg
    22681 attcttccca tacccaccaa ttaatcccgg atcggttggt ccataaaccg tgatattggg
    22741 tctatccagt gccgccgtta aatggcttaa ccccgtatcc accgacacta caaatttagc
    22801 cccggccagc acgcgggcaa cgccttccag actcatcttc ggcaatactt caacataatc
    22861 aaatccttcc gccagtcgtt tcgcccgttc ttcctcatgc ggcgcgcccc acggaagttt
    22921 aatccgtatt cctgaatcag ccagtaaacc aatcaattct cgccagtgtt cttccggcca
    22981 gtgtttatca tcacgggtcg tcgcatgaag aaatacggca tattcgccag catctgtagg
    23041 caggttcgtc agaaaatgct gtgcgatagc ataatcgccc tgggtttgcg gtttgctata
    23101 gcccaaactt ttggcaaaca gttcgcgggt gcgttctacg gcgtgctgct gttttgcaat
    23161 atgatgctta caattgtaaa acaggctggc taaaggttcg cgagcggttt gccagtccaa
    23221 tccatgcttt acgccatgcg ccagacgtgt caccagtgcc gcgcttttta ccagcccctg
    23281 agcgtcgata actgcgtcat agttctctgc ttgtagcgct tcacgaaacg ctttgcgttc
    23341 agcttttatg ggggccgaga accaggcttt acgccagcga cgtattgcca caggaataac
    23401 tcgctcaacg gcagcgtgcc aggaaggaat ctgtgcgaac ccttcttcca ccacccagtc
    23461 aaacttaatc cctgggattg cctgctgggc atcagtgagt gcgggcaacg tatggagaac
    23521 atcgcccatc gacgatgttt taacgatcaa aacccgcatc cgtcaggctt cctcttgtaa
    23581 caatagcgtg ttgagttctt ccagtacgcg ctggggagta atgtcgatca agctctggtg
    23641 ataaccctcc gcagcgtcac ctttacgcac tttgtgatag ccggtaatca gacggatcac
    23701 gcgcgcttta tgggatagcg gcggtgtgaa gtccgggcta cttggaccat acagggcaac
    23761 cagcggacga ttgagcgccg ccgcaacgtg catcaggcca gaatcgttag tgacaatggc
    23821 tttacaggct gcaatcagga taaccgcttg atcaagctgt gtttcccccg ccaggttccg
    23881 acaccatgcc tgctgctcgg tattcaaagc ggcaagaatc tcattgcccg cttcatgatc
    23941 tttcgcagag ccaaacagaa ccacctgata accttcatca atcagctgct ttgccagttc
    24001 cgcatagtgg tagtgtggcc agcgttttgc cggaccaaac tccgcacccg ggcaaaagcc
    24061 aatcatcgga cgttctgatg aaagcgaaaa ttgattacag gtatatgatt tttcaccttc
    24121 gctcacctgc aactgcggcc ataacaatgg ctgcggcaga tcttgtgcgg tacgcataat
    24181 gcctttgtca taggccagcg cgacatagcg ttccaccatt agcggccagg cttctttatc
    24241 gagcacgcgt acatcgttga gtaaaccgta gcgcatctcg ccgcgccagc cggtgcgatg
    24301 aggaataccc gcgaagaaag gcactaatgc agatttgaag gagttgggta agacgtaggc
    24361 gcggtcgtag cgcttttcac gcaggctatg acccagtttg cggcgttcgc cgatttccag
    24421 cgctccgtga ccgagaggca tagggatagc ttcgttaact tccggcatcc gcgataataa
    24481 tggacggcac catgccggtg ccatcacatc gattatcgcc tggggatagc gcgcctggag
    24541 cgtgcgatag agactttgcg acatcatcat gtcgccaacc caagacgggc cgatcaccag
    24601 tattttcatg cagagctctt atgcgtcgcg attcagccag gccatgtatt ccgttacacc
    24661 ttcagcaacg gttttgaacg gtttgtcgta acccgccgcg cgcagatttg tcagatctgc
    24721 ctgagtgaac gcctggtagc ggcctttcag cttatccggg aacggaatgt attcgatctg
    24781 gcctttcttg tgataagcca gagtagcatc tgctacggcc tggaaggatt ccgcacgacc
    24841 agtaccgagg ttgaagatac cggaaacgcc attttccagg aaccacaggt ttacatctgc
    24901 cacgtcgccc acatagacga agtcacgttt gaagttctcg ctaccttcaa acagcttcgg
    24961 tgattcaccg ttgttaagct gagtgttgag atggaaagcg acgctcgcca tgctgccttt
    25021 atggccttca cgcggtccat aaacgttgaa atagcggaag ccaacaatct gcgagttcgc
    25081 ttcaggcagg atttggcgaa catattcatc aaacaggaat tttgagtaac cgtagacgtt
    25141 caacggtttt tcgtactcgc gggattcaat aaagtcggag gtgcgtccgc cgtaggtggc
    25201 tgcggaagaa gcgtacagga acgggatttc gcgctccagg cagtagtgca gcagctcctt
    25261 ggagtattga tagttgttat ccatcatata cttgccgtcc cactcggtgg tggaagagca
    25321 tgcgccttcg tggaaaatcg cttcgacatc gccgaactct tcgccagcca taatctggat
    25381 caggaagtct tccttatcca tatagtccgc gatatccaga tccaccaggt tcacaaactt
    25441 ggtgccgtct ttcaggttgt ccaccaccag aatatcggtg atgcctttat cattcagggc
    25501 cttaacgatg ttgctgccga taaagcccgc gccgccggta acgatgatca taactgtaac
    25561 cttcgaatta tggagtcaga gacaatctca gacacgaata cttctatcat atcactatac
    25621 ggcgttggct tcagccattc gccaaaagtg agattgatgg acgttattta tcctgaattt
    25681 tgcagaagtg ttaacgcgtc atctcgtcgc gacgtataag tttgggtaat atgtgctgga
    25741 atttgccctg tctggagaat cgcaatgcgt ggagaatttt atcagcagtt aaccaacgat
    25801 ctggaaaccg cacgggcgga agggttgttt aaagaagagc gcattattac gtctgcgcag
    25861 caagcagata tcactgtggc tgatggaagc cacgtcatta acttttgtgc caacaactat
    25921 ctcgggctgg cgaatcatcc ggatctgatt gcggcagcaa aggcgggaat ggattctcac
    25981 ggtttcggca tggcttcggt gcgttttatt tgcggcactc aggacagcca taaagagctt
    26041 gaacaaaaac tggcggcatt cctggggatg gaagatgcga ttctctactc ttcctgcttt
    26101 gatgctaacg gtggactgtt tgaaacgctg ctgggtgcgg aagacgccat tatctccgac
    26161 gcactgaacc acgcgtctat tattgatggt gtgcgtctgt gcaaagctaa acgctatcgc
    26221 tatgccaaca acgatatgca ggagctggaa gcacgtctga aagaagcgcg tgaagccggt
    26281 gcgcgtcatg tgctgattgc caccgatggt gtgttctcaa tggacggcgt gattgccaac
    26341 ctgaaaggcg tttgcgatct ggcagataaa tacgatgcct tggtgatggt ggatgactcc
    26401 cacgcggtcg gttttgtcgg tgaaaatggt cgtggttccc atgaatactg cgatgtgatg
    26461 ggccgggtcg atattatcac cggtacgctt ggtaaagcgc tgggcggggc ttctggtggt
    26521 tataccgcgg cgcgcaaaga ggtggttgag tggctgcgcc agcgttctcg tccgtacctg
    26581 ttctccaact cgctggcacc ggccattgtt gccgcgtcca tcaaagtact ggagatggtc
    26641 gaagcgggca gcgaactgcg tgaccgtctg tgggcgaacg cgcgtcagtt ccgtgagcaa
    26701 atgtcggcgg cgggctttac cctggcggga gccgatcacg ccattattcc ggtcatgctt
    26761 ggtgatgcgg tagtggcgca gaaatttgcc cgagagctgc aaaaagaggg catttacgtc
    26821 accggtttct tctatccggt cgttccgaaa ggtcaggcgc gtattcgtac ccagatgtct
    26881 gcggcgcata cccctgagca aattacgcgt gcagtagaag cattcacgcg tattggtaaa
    26941 caactgggcg ttatcgcctg aggatgtgag atgaaagcgt tatccaaact gaaagcggaa
    27001 gagggcatct ggatgaccga cgtccctgta ccggaactcg ggcataacga tctgctgatt
    27061 aaaatccgta aaacagccat ctgcgggact gacgttcaca tctataactg ggatgagtgg
    27121 tcgcaaaaaa ccatcccggt gccgatggtc gtaggccatg aatatgtcgg tgaagtggta
    27181 ggtattggtc aggaagtgaa aggcttcaaa atcggcgatc gcgtttctgg cgaaggccat
    27241 atcacctgcg gtcactgtcg caactgtcgt ggcggtcgta cccatctgtg ccgcaacacg
    27301 atcggcgtcg gcgttaaccg tccgggctgc ttcgccgaat atctggtgat cccggcgttc
    27361 aacgccttca aaatcccaga caatatctcc gacgacctgg cttccatttt tgatcccttc
    27421 ggtaacgccg tgcataccgc gctgtcgttc gatctggttg gcgaggatgt gctggtctct
    27481 ggtgcaggtc cgataggtat tatggctgcg gcggtggcga aacacgttgg tgcacgcaat
    27541 gtggtgatca ctgatgttaa cgaataccgc cttgagctgg cgcgcaaaat gggtatcacc
    27601 cgtgcggtta acgtcgcgaa agaaaacctt aatgatgtga tggctgaact gggcatgacc
    27661 gaaggctttg atgtcggtct ggaaatgtcc ggtgcgccgc cagcgtttcg taccatgctt
    27721 gacaccatga accacggcgg ccgtattgcg atgctgggta ttccgccgtc tgatatgtct
    27781 atcgactgga ccaaagtgat ctttaaaggc ttgttcatta aaggtattta cggtcgtgag
    27841 atgtttgaaa cctggtacaa gatggcggcg ctgattcagt ctggcctcga tctctcgccg
    27901 atcattaccc atcgtttctc tatcgatgat ttccagaagg gctttgacgc tatgcgttcg
    27961 ggccagtccg ggaaagtaat tctgagctgg gattaacacg aacaagggct ggtattccag
    28021 cccttttatc tggggataat ctgttgaata tgtaaaatcc tgtcagtgta ataaagagtt
    28081 cgtaattgcg ctgatctctt atatagctgt tctcattatc tctctaccct gaagtgactc
    28141 tctcacctgt aaaaataata tctcacaggc ttaatagttt cttaatacaa agcctgtaaa
    28201 acgtcaggat aactccagag gtcgtcggta atttatgatg aacagcacca ataaacttag
    28261 tgttattatt ccgttatata atgcgggcga tgatttccgc acttgtatgg aatctttaat
    28321 tacgcaaacc tagactgctc tggaaatcat tattattaac gatggttcaa cggataattc
    28381 tgttgaaata gcaaagcatt acgcagaaaa ctatccgcac gttcgtttgt tgcatcaggc
    28441 gaatgctggc gcatcggtag cgcgtaatcg tgggatcgag gtggcgacgg gcaaatatgt
    28501 cgcttttgtc gatgctgacg atgaggtcta tcccaccatg tacgaaacgc tgatgaccat
    28561 ggcgttagag gacgacctcg acgtggcgca gtgcaacgct gactggtctt ttcgtgaaac
    28621 gggagaaacc tggcaatcca tccccagcga tcgccttcgc tcaaccggcg tattaaccgg
    28681 cccggactgg ctgcggatgg ggctttcttc gcgccgttgg actcacgttg tctggatggg
    28741 ggtttatcgc cgtgatgtta ttgttaaaaa taacattaaa tttattgccg gattacatca
    28801 tcaggatatt gtctggacaa cagaattcat gtttaacgcg ctgcgtgcgc gatataccga
    28861 gcaatcatta tataaatatt atctgcataa tacgtcagtg agtcggttgc atagacaagg
    28921 gaataaaaac cttaattatc aacgtcacta tattaagatt acccgcctgc tggagaaatt
    28981 aaatcgaaat tatgccgaca aaattacgat ttatccggaa tttcatcagc aaataactta
    29041 cgaagcattg cgtgtttgcc atgcggtgcg caaagagccg gatattctta cccgccaacg
    29101 gatgattgcc gagatattta cttccggtat gtataagcgc ctgattacca atgtgcgcag
    29161 cgtgaaggtc ggttaccagg cgttactgtg gtctttccgc ttatggcaat ggcgcgacaa
    29221 aacgcggtcg caccatcgca ttacgcgtag cgcctttaat ttgcgctagc gttaaacttc
    29281 gccgcttcgg gctgtttgcc ccagccttgc cactgatgct ggaagtaaac gatcagcgtg
    29341 ctctggctga tgctttcgct taatacttca aagaagcgat tagcataaac aggttctatc
    29401 ggtttcttcg gtttgcacag cttcacgcca cggaacggat tacgcggtgc atcaggcaca
    29461 gcgtttttcg gcggtgtaga agtatcaacc tgcggttcat tcagcaagct gctggctttc
    29521 accagcgtaa tgtctggcgg caggttataa accatctgtt gtagcacgcg taccgttaaa
    29581 gggtgaggat gcccaatggc aatggttgaa ccgttgcgac gcgccagatc aattgcgcga
    29641 ttaaattgca cacggatgtc cgcttcgttc tgcgaatcgt cgaggaacac cttccgttta
    29701 ataaccttca cgccagtacc ttgcgcggcg cgcatcgcct gggtattacc gatggttacg
    29761 ctgtcgagga agtaaagatt gtaacgctcc agtgcctgca tcactttctg cataccaaac
    29821 aggttagagg tcatcttgct acccatgtgg ttgttgatcc ccacggcata gggcacgtta
    29881 ttgaccgcac tacgaataat gcgctcaatt tcgtcgctgc tcatctccgg gcgcagcgta
    29941 tttttctcca gcggctgttt actcagtggt gccatcggga gatgaatcaa cacttcgtgc
    30001 ccgctgttat gcgctttggt cgccatctct ctggcgtgcg gtgaatcggg taatacagcg
    30061 acggagatag cggagggcat cgccagcacc tggttttcgt tgtgcgggcg atacccaaaa
    30121 tcatcaatga cgatggcaag tttgccagca agtacggggg aggagagcgc caacagagcg
    30181 gcaaatgcaa gaacgttacg acgaaatgga aacaaaactt atcttcccaa ccacggctgt
    30241 ggattgaccg cctgaccctg gcggcgaatt tcgaaataga gtgaaggccg accctgaccg
    30301 ccactgctgc ccaccagtgc aattggctgg cccgcgcgaa cctgcgaacc aacgctcacc
    30361 agtgcgctct gattatagcc gtaaagactc atgtcgcctt taccgtgctc aaccaccacc
    30421 accagaccgt aaccttgcag ccagtcagcc agaatcactc gaccgtcggc aattgcttta
    30481 acttcagtac cttcagaggc accgataacc atccctttcc agcgtaattc accctgtagc
    30541 tgctcgccat agcgatgcag cgtcggcccg cgaaccggcc agaatgcctg accacgcggc
    30601 gcacccagac caccagttcg ggacatcagc gatttttcgc tttcggtcgg tttgtaggtg
    30661 gtgcctttgc gcgtcgcttc tttctggcgg tcgcgaaccg cctgggcctc acgcgcttct
    30721 cgttcagcac gcgctttcgc cgcggcttcc gcacgggcaa tgctgttacg cagacgggat
    30781 tcgttggcac gcagttcact tagctgctgc tgaccttgct ggatggaaga ttccagcccc
    30841 gccagcgtct ttttacgctc gttcagagcc tgagtcagtt tcgcctgttg ggcgcgctgt
    30901 tcatataaca gcgtttgttg ctcgctttgt ttctcttcca gttcagcacg ctgcatggcg
    30961 acttcttcac gcgtttgctt caactgggca atggtttctt gtcgcgcctg gttgagatag
    31021 ccgaaataag cctgtaaacg ctgtccacac tggctttctt caccgctgag aattagctga
    31081 ataccggtat gctcgccctg acggaatgcg gcatccagtt gtgcggcgag gctgcgctcc
    31141 tgggcggctt tttgctgctc cagtttggca atcgacgcgt tcatctcatc aatctgttta
    31201 ttgagttgat tgagcgtgtt ttgcgtttcg cgcagcttac gggtggcttc agagatcgct
    31261 tcttcctgct ttttcaattg tgcgagcagg cttgcgcgtt gttgttgctt ttggcgtacc
    31321 gcgcgctctt ttgcggcgat atcggcctga atagatttga gttggtcacg ctcatccgcg
    31381 tgggcggaaa aggcgcacaa aaatacgcca gcgctaagaa cgctggcgta gatgatgggc
    31441 ctgattgcaa acctgcgcgg tttcacggcc cgtgtcatgg tattaatcgc ctttcccctc
    31501 atggggaggg attattccac gatgaacagc ggcttaccag tcatctcttg cgggatttcc
    31561 atacccatca gcgacaacat ggtcggcgcg atgtcagaaa gtttgccgcc tgcaaccgct
    31621 ttcacgttct tatcaccaac gtaaatcagc ggaactggca ggttggtgtg tgccgtgtgt
    31681 gcctgacccg ttgccggatc gcgcatctgt tcagcgttac cgtggtcagc ggtgatcagc
    31741 agttgtccac ccacggactc aaccgctttc gcgacttctt ccacgcagtg atccagtgct
    31801 tcaaccgctt taaccgccgc ttccatcacc ccggtgtgac ctaccatgtc gccgttcgga
    31861 tagttacaga taatggtgtc gtatttgccg ctcttgatgg ccgcaaccag tttttcggtc
    31921 agctctgcgg agctcatttc cggttgcaga tcgtaggtag ccactttcgg tgagttgatc
    31981 agaatgcgat cttcgccttt gaatgactct tctacgccac cgttgaagaa gaaagtaacg
    32041 tgggcatatt tttcggtttc ggaaatacgc aactgagttt tgtcgttttt cgccatccac
    32101 tcgccgaagg tgttaaccag ggaagcgggt gggtaagcaa ccgcagtttt gatgtcagcg
    32161 gcgtattcgg tcagcatcac gaaatcgacg ttaaccactt tcttacgcgc gaagccgtcg
    32221 aaatctgcgt ttacgaatgc acgagtgatt tcgcgcgcgc ggtcagcacg gaagttcatg
    32281 aaaatcagcg catcgccgtc ttccattgcc gcatctggct gaccttctgc acgaataacg
    32341 gtcgctttca cgaactcgtc gttttcgtcg cgagcataag ctgcctgcaa accagcaacg
    32401 gcagtatcgg cctggaactc accctgcgcc agagtcagca ggtcataggc tttttcaacg
    32461 cgatcccaac ggttatcgcg gtccatcgcg tagtaacgac caatgatgga cgctacgcgg
    32521 cctttaccca gcgcagcaaa tttctcttca aattttttca gcgaggattc agcactgcgc
    32581 ggcggggtgt cgcgaccgtc aaggaatgcg tgcaggtaga ttttttctgc gccgcgttca
    32641 gctgccagtt ctaccatcgc catgatgtga tcttcgtggc tgtgtacgcc gcctgcggag
    32701 agcagaccca taatgtgtac tgctttacct gcgtttttcg ctttatctac cgcgccagtc
    32761 agcactggat tagcaaagaa agcgcgatct ttgatttcaa cgtccagacg agtcaggtcc
    32821 tgatacacga tgcggccggc acccaggtta acgtggccta cttcggagtt acccatctga
    32881 cggtcaggca gaccgacttc cagaccggaa gcgtcgatca gggtatgcgg acgattggcc
    32941 cacagtgcat ccattaccgg ggttttagca ctaaaaatgg cgttatcctg ctgttcttca
    33001 cgatagccat agccatccag aatcaccagt accataggtt ttttagaaac cgacattgcg
    33061 acaacctcat actcaagagt caaaatttgc gtaattttac tacagctgaa tcgataaaat
    33121 agcctctgaa gatcaaaata tacgaccgct cgcggcaagc cgatgccaga tttgcgcttt
    33181 tttttcgtcg tgccccgcaa aattgcattc cagttaacgc gctggctgta tttgccgcac
    33241 cgcgcaggta tactcctttc ctggtttttt taatcactac gtcgggagtt gttacccccc
    33301 atgcaagaaa ttatgcaatt tgttggccgt catcccatac tgagtatcgc ttggatcgcg
    33361 ttactggtgg cggttcttgt gactacgttt aagagcctga cctcgaaagt gaaggtgatt
    33421 actcgtggtg aagctacgcg tctgatcaac aaagaagacg ctgtggttgt ggatttacgt
    33481 cagcgtgatg acttccgtaa aggccatatc gcaggttcta tcaatctgtt gccgagcgaa
    33541 atcaaagcca acaatgttgg tgagcttgag aagcacaaag acaaaccggt tatcgtggta
    33601 gacggttctg gcatgcagtg ccaggagcct gcaaacgcgc tgacgaaagc tggttttgcg
    33661 caagtattcg tgctgaaaga gggcgttgct ggctgggctg gcgaaaactt gcctctggtg
    33721 cgcggcaaat aatttacctg tcgtctttca tgatgcaggc cgttagctgc aattaggccc
    33781 ccagcgactt attgttataa gcttctgggg acactccatt ttgcagccgc cctgcaacat
    33841 gaaatttcag ggtaaaaaaa caggagctaa attatggcca atgttgaaat ctataccaaa
    33901 gaaacctgcc cgtattgcca tcgtgcaaaa gcactgctga gcagcaaggg cgtgagtttt
    33961 caggagttgc cgatcgatgg caatgccgcc aagcgtgaag agatgatcaa acgcagcggt
    34021 cgcaccacgg ttccccagat ttttattgac gcacagcaca ttggcggctg tgatgacttg
    34081 tatgcattgg atgcacgtgg tggactggat cccctgctga aataacgtgt gaacgttggc
    34141 attacattgc gcagtattta aggacaacac ttaagggttt tctacacatg tcagaacaaa
    34201 acaacactga aatgactttc cagatccaac gtatttatac caaggatatc tctttcgaag
    34261 cgccgaacgc gccgcacgtt ttccagaaag attggcaacc agaagttaaa cttgatctgg
    34321 atacggcatc ttcccaactg gcagatgacg tatacgaagt ggtactgcgt gttaccgtaa
    34381 cggcctccct gggcgaagaa accgcgttcc tgtgtgaagt tcagcagggc ggtattttct
    34441 ccatcgcggg tatcgaaggc acccagatgg cacattgcct gggcgcatac tgcccgaaca
    34501 ttctgttccc gtatgctcgt gagtgcatca ccagcatggt atcccgcggt acattcccgc
    34561 aactgaacct tgcgccggtt aacttcgatg cgctgttcat gaactatttg cagcagcagg
    34621 ctggcgaagg tactgaagaa catcaggatg cctgatgaac caacgtaatg cttcaatgac
    34681 tgtgatcggt gccggctcgt acggcaccgc tcttgccatt accctggcaa gaaatggcca
    34741 cgaggttgtc ctctggggcc atgaccctga acatatcgca acgcttgaac gcgaccgctg
    34801 taacgccgcg tttctccccg atgtgccttt tcccgatacg ctccatcttg aaagcgatct
    34861 cgccactgcg ctggcagcca gccgtaatat tctcgtcgtc gtacccagcc atgtctttgg
    34921 tgaagtgctg cgccagatta aaccgctgat gcgtcctgat gcgcgtctgg tgtgggcgac
    34981 caaagggctg gaagcggaaa ccggacgtct gttacaggac gtggcgcgtg aggccttagg
    35041 cgatcaaatt ccgctggcgg ttatctctgg cccaacgttt gcgaaagaac tggcggcagg
    35101 tttaccgacg gctatttcgt tggcctctac cgaccagacc tttgccgatg atctccaaca
    35161 attgctgcac tgtggcaaaa gtttccgcgt ttacagcaac ccggatttca tcggtgtgca
    35221 gcttggcggt gcggtgaaaa acgtcattgc cattggcgcg gggatgtccg acggtatcgg
    35281 ttttggtgcg aatgcgcgta cggcactgat cacccgtggg ctggctgaaa tgtcgcgtct
    35341 tggcgcggcg ctgggtgccg agcctgccac ctttatgggc atggcggggc tgggcgatct
    35401 tgtgcttacc tgtaccgaca accagtcgcg taaccgccgt tttggcatga tgctcggtca
    35461 gggcatggat gtacaaagcg cgcaggagaa gattggtcag gtggtggaag gctaccgcaa
    35521 tacgaaagaa gtccgcgaac tggcgcatcg cttcggcgta gaaatgccaa taaccgagga
    35581 aatttatcaa gtattatatt gcggaaaaaa cgcgcgcgag gccgcattga cgttattagg
    35641 tcgtgcacgc aaggacgagc gcagcagcca ctaaccccag ggaacctttg ttaccgctat
    35701 gacccggccc gcgcagaacg ggccggtcat tatctcatcg tgtggagtaa gtaatgtcgt
    35761 gtgaagaact ggaaattgtc tggaacaata ttaaagccga agccagaacg ctggcggact
    35821 gtgagccaat gctggccagt ttttaccacg cgacgctact caagcacgaa aaccttggta
    35881 gtgcactgag ctacatgctg gcgaacaagc tgtcatcgcc aattatgcct gctattgcta
    35941 tccgtgaagt ggtggaagaa gcctacgccg ctgacccgga aatgatcgcc tctgcggcct
    36001 gtgatattca ggcggtgcgt acccgcgacc cggcagtcga taaatactca accccgttgt
    36061 tatacctgaa gggttttcat gccttgcagg cctatcgcat cggtcactgg ttgtggaatc
    36121 aggggcgtcg cgcattggca atctttctgc aaaaccaggt ttctgtgacg ttccaggtcg
    36181 atattcaccc ggcagcaaaa attggccgtg gcattatgct cgaccacgcc acgggcatcg
    36241 ttgtgggtga aacggcggta attgaaaacg acgtatcgat tctgcaatct gtgacgcttg
    36301 gcggtacggg taaatctggt ggtgaccgtc acccgaaaat tcgtgaaggt gtgatgattg
    36361 gcgcgggcgc gaaaatcctc ggcaatattg aagttggtcg cggcgcgaag attggcgcag
    36421 gttccgtggt gctgcaaccg gtgccgccgc atacaaccgc cgctggcgtt ccggctcgta
    36481 ttgtcggtaa accagacagc gataagccat caatggatat ggaccagcat ttcaacggta
    36541 ttaaccatac atttgagtat ggggatggga tctaatgtcc tgtgatcgtg ccggatgcga
    36601 tgtaatcatc tatccggcat acagtaacta atctctcaac aacgctcccg gatatcccag
    36661 ctgccgccag gcttcataca ccactaccga caccgcattg gacagattca tgctgcggct
    36721 gtccggcacc atcggaatgc gaattttttg ttcagcgggc agggcatcaa gaatgctcgc
    36781 tggcaggccg cgtgtttccg ggccgaacat cagataatcg ccatcctgat agcttacggc
    36841 gctgtgagca ggcgtacctt tcgtggtgag ggcaaacagg cgctggggat tttctgcttc
    36901 gaggaacgcg cgatagtcat gatgacgcgt aacggcggta aactcgtgat agtccagccc
    36961 cgcgcggcgc aggcgcttat cgtcccaggc aaatcccatc ggttcgatga tatgcagacg
    37021 aaagccggta ttagcgcaaa gacggatgat gttgccagta tttggcggaa tttctggttc
    37081 gtaaagtacg atgtttagca tactgccccc ttagtgcggg ggcaggatag cagaaaattg
    37141 gtgaaccgtg cggccttttc ccctcacccc ggccctcccc ctgtctcttg atcaggagat
    37201 ctgatcaaga gacagctccc tgagggagag ggt
//
LOCUS       DADLFA010000016        36828 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000016 DADLFA010000000
VERSION     DADLFA010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36828
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.016"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            585..671
                     /gene="leuL"
                     /locus_tag="JQH65_000711"
     CDS             585..671
                     /gene="leuL"
                     /locus_tag="JQH65_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HAY6138213.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            764..2335
                     /gene="leuA"
                     /locus_tag="JQH65_000712"
     CDS             764..2335
                     /gene="leuA"
                     /locus_tag="JQH65_000712"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HAY6138214.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2335..3426
                     /gene="leuB"
                     /locus_tag="JQH65_000713"
     CDS             2335..3426
                     /gene="leuB"
                     /locus_tag="JQH65_000713"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HAY6138215.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDITGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3429..4829
                     /gene="leuC"
                     /locus_tag="JQH65_000714"
     CDS             3429..4829
                     /gene="leuC"
                     /locus_tag="JQH65_000714"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HAY6138216.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGQVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4840..5445
                     /gene="leuD"
                     /locus_tag="JQH65_000715"
     CDS             4840..5445
                     /gene="leuD"
                     /locus_tag="JQH65_000715"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HAY6138217.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(5494..6576)
                     /locus_tag="JQH65_000716"
     CDS             complement(5494..6576)
                     /locus_tag="JQH65_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY6138218.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNTIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNAPLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSIAAGIFTLSLVLIAFML
                     PSVARVELPSENALSMQGGWQDSNVRMLFVASTLMWTCNTMYIIDMPLWISSELGLPD
                     KLAGFLMGTAAGLEIPAMILAGYYVKRYGKRRMMVIAVAAGVLFYTGLIFFHSRMALM
                     TLQLFNAVFIGIVAGIGMLWFQDLMPGRAGAATTLFTNSISTGVILAGVIQGAIAQSW
                     GHFAVYWVIAVISVVALFLTAKVKDV"
     gene            complement(6678..6809)
                     /gene="sgrT"
                     /locus_tag="JQH65_000717"
     CDS             complement(6678..6809)
                     /gene="sgrT"
                     /locus_tag="JQH65_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HAY6138219.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            6898..8553
                     /locus_tag="JQH65_000718"
     CDS             6898..8553
                     /locus_tag="JQH65_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SgrR"
                     /protein_id="HAY6138220.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVNYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8587..8686
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8717..9700
                     /gene="thiB"
                     /locus_tag="JQH65_000719"
     CDS             8717..9700
                     /gene="thiB"
                     /locus_tag="JQH65_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HAY6138221.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISGWQRAVSR"
     gene            9676..11286
                     /gene="thiP"
                     /locus_tag="JQH65_000720"
     CDS             9676..11286
                     /gene="thiP"
                     /locus_tag="JQH65_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HAY6138222.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLYQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPDEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLQQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11270..11968
                     /gene="thiQ"
                     /locus_tag="JQH65_000721"
     CDS             11270..11968
                     /gene="thiQ"
                     /locus_tag="JQH65_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HAY6138223.1"
                     /translation="MLKLTDITWLYQHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(12081..12845)
                     /locus_tag="JQH65_000722"
     CDS             complement(12081..12845)
                     /locus_tag="JQH65_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY6138224.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(12931..13809)
                     /gene="araC"
                     /locus_tag="JQH65_000723"
                     /pseudo
     CDS             complement(12931..13809)
                     /gene="araC"
                     /locus_tag="JQH65_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
     gene            14148..15848
                     /gene="araB"
                     /locus_tag="JQH65_000724"
     CDS             14148..15848
                     /gene="araB"
                     /locus_tag="JQH65_000724"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HAY6138225.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFDRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLNHLP
                     VVLNWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQTQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            15859..17361
                     /gene="araA"
                     /locus_tag="JQH65_000725"
     CDS             15859..17361
                     /gene="araA"
                     /locus_tag="JQH65_000725"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAY6138226.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17561..18256
                     /gene="araD"
                     /locus_tag="JQH65_000726"
     CDS             17561..18256
                     /gene="araD"
                     /locus_tag="JQH65_000726"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAY6138227.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMEIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            18331..20682
                     /gene="polB"
                     /locus_tag="JQH65_000727"
     CDS             18331..20682
                     /gene="polB"
                     /locus_tag="JQH65_000727"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HAY6138228.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGSENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20846..23752
                     /gene="rapA"
                     /locus_tag="JQH65_000728"
     CDS             20846..23752
                     /gene="rapA"
                     /locus_tag="JQH65_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HAY6138229.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHMPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIESLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLLDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23764..24423
                     /gene="rluA"
                     /locus_tag="JQH65_000729"
     CDS             23764..24423
                     /gene="rluA"
                     /locus_tag="JQH65_000729"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HAY6138230.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24540..25355)
                     /gene="djlA"
                     /locus_tag="JQH65_000730"
     CDS             complement(24540..25355)
                     /gene="djlA"
                     /locus_tag="JQH65_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HAY6138231.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25610..27964
                     /gene="lptD"
                     /locus_tag="JQH65_000731"
     CDS             25610..27964
                     /gene="lptD"
                     /locus_tag="JQH65_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HAY6138232.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWQHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVEHFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            28017..29303
                     /gene="surA"
                     /locus_tag="JQH65_000732"
     CDS             28017..29303
                     /gene="surA"
                     /locus_tag="JQH65_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HAY6138233.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            29303..30292
                     /gene="pdxA"
                     /locus_tag="JQH65_000733"
     CDS             29303..30292
                     /gene="pdxA"
                     /locus_tag="JQH65_000733"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HAY6138234.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMILNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            30289..31110
                     /gene="rsmA"
                     /locus_tag="JQH65_000734"
     CDS             30289..31110
                     /gene="rsmA"
                     /locus_tag="JQH65_000734"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAY6138235.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            31113..31490
                     /gene="apaG"
                     /locus_tag="JQH65_000735"
     CDS             31113..31490
                     /gene="apaG"
                     /locus_tag="JQH65_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HAY6138236.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31497..32339
                     /gene="apaH"
                     /locus_tag="JQH65_000736"
     CDS             31497..32339
                     /gene="apaH"
                     /locus_tag="JQH65_000736"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase
                     (symmetrical)"
                     /protein_id="HAY6138237.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(32417..32896)
                     /gene="folA"
                     /locus_tag="JQH65_000737"
     CDS             complement(32417..32896)
                     /gene="folA"
                     /locus_tag="JQH65_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HAY6138238.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(33088..34950)
                     /gene="kefC"
                     /locus_tag="JQH65_000738"
     CDS             complement(33088..34950)
                     /gene="kefC"
                     /locus_tag="JQH65_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HAY6138239.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(34943..35473)
                     /gene="kefF"
                     /locus_tag="JQH65_000739"
     CDS             complement(34943..35473)
                     /gene="kefF"
                     /locus_tag="JQH65_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HAY6138240.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGKALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(35555..>36828)
                     /locus_tag="JQH65_000740"
     CDS             complement(35555..>36828)
                     /locus_tag="JQH65_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005116114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY6138241.1"
                     /translation="DGYVLVMIGVALEQLTPALKLDADWIGLLGAGTLAGLFVGTSLF
                     GYISDKVGRRKMFLIDIIAIGVISVATMFVSSPVELLVMRVLIGIVIGADYPIATSMI
                     TEFSSTRQRAFSISFIAAMWYVGATCADLVGYWLYDVEGGWRWMLGSAAIPCLLILIG
                     RFELPESPRWLLRKGRVKECEEMMIKLFGEPVAFDEEQPQQTRFRDLFNRRHFPFVLF
                     VAAIWTCQVIPMFAIYTFGPQIVGLLGLGVGKNAALGNVVISLFFMLGCIPPMLWLNT
                     AGRRPLLIGSFAMMTLALAVLGLIPDMGIWLVVMAFAVYAFFSGGPGNLQWLYPNELF
                     PTDIRASAVGVIMSLSRIGTIVSTWALPIFINNYGISNTMLMGAGISLFGLLISVAFA
                     PETSRDVIGANQQYDDPRAENGVNCSDFSLF"
BASE COUNT         8963 a        10084 c         9687 g         8094 t
ORIGIN      
        1 catgcatata aaataagaaa aagcaaaatg agtaaaattc ggaaaacata aagatacggg
       61 ttctgataaa aacagaatcc attgccgtgg aatgagtcat ttaccacagc ataataatcc
      121 ataatgtttt tgttgcgaaa acaatctaat cataactacc gcgaatactc aatcatctac
      181 aaaatggatt aaatgtgctt atttaataat taatttatat atttaatgca ttaattatta
      241 acattaatgg atcaataata ttcaccatat caatatcaaa aaaaaattcg caaaacatat
      301 aattcaatac aaatcatcag gataggtttt gcaacgcgtg cattttgtcc cctttttcct
      361 cgttgattag atgcaaaaat ttattctgaa atatgtcaac ctatgaaaag tgtcggtcgt
      421 taaacagaaa ttaatatcgc ttgctttaac caccgcagca caattagcta attttacgga
      481 tgcagaactc gcgctggcgg gacgttttta ttgcgtcagg gttgacatcc gtttttgtat
      541 ccagtaactc taaaagcata tcgcattcat ctggagctga tttaatgact cacatcgttc
      601 gctttatcgg tctactacta ctaaacgcat cttctttgcg cggtagacga gtgagcggca
      661 tccagcatta agccagcacg cagtcaaaca aaaaacccgc gccattgcgc gggttttttt
      721 atgcccgaag cgaggcgctc taaaagagac aaggacccaa accatgagcc agcaagtcat
      781 tattttcgat accacattgc gcgacggtga acaggcgtta caggcaagct tgagtgtgaa
      841 agaaaaactg caaattgcgc tggcccttga gcgtatgggt gttgacgtga tggaagtcgg
      901 tttccccgtc tcttcgccgg gcgattttga atcagtgcaa accatcgccc gccaggttaa
      961 aaacagccgc gtatgcgcgt tagctcgctg cgtggagaaa gatatcgacg tggcggccga
     1021 atccctgaaa gtcgccgaag ccttccgtat tcataccttt attgccactt cgccaatgca
     1081 catcgccacc aagctgcgca gcacgctgga tgaagtgatc gaacgcgcta tctatatggt
     1141 gaaacgcgcc cgtaattaca ccgatgatgt tgaattttct tgcgaagatg ccgggcgtac
     1201 ccccattgcc gatctggcgc gagtggtcga agcggcgatt aatgccggtg ccaccaccat
     1261 caacattccg gacaccgtgg gctacaccat gccgtttgag ttcgccggaa tcatcagcgg
     1321 tctgtatgaa cgcgtaccta acatcgacaa agccattatt tccgtacata cccacgacga
     1381 tttaggcctg gcggtcggca actcactggc ggcggtacat gccggtgcac gccaggtgga
     1441 aggtgcaatg aacgggatcg gcgagcgtgc cggtaactgt tcgctggaag aagtcatcat
     1501 ggcgattaaa gttcgtaagg atattctcaa cgtccacacc gccattaatc accaggagat
     1561 atggcgcacc agccagttag ttagccagat ttgtaatatg ccgatcccgg caaacaaagc
     1621 cattgttggc agcggcgcat tcgcccactc ctccggtatc caccaggatg gcgtgctgaa
     1681 aaaccgcgaa aactacgaaa tcatgacacc agaatctatt ggtctgaacc aaatccagct
     1741 gaatctgacc tctcgttcgg ggcgtgcggc ggtgaaacat cgcatggatg agatggggta
     1801 taaagaaagt gaatataatt tagacaattt gtacgatgct ttcctgaagc tggcggacaa
     1861 aaaaggtcag gtgtttgatt acgatctgga ggcgctggcc ttcatcggta agcagcaaga
     1921 agagccggag catttccgtc tggattactt cagcgtgcag tctggctcta acgatatcgc
     1981 caccgccgcc gtcaaactgg cctgtggcga agaagtcaaa gcagaagccg ccaacggtaa
     2041 cggtccggtc gatgccgtct atcaggcaat taaccgcatc actgactata acgtcgaact
     2101 ggtgaaatac agcctgaccg ccaaaggtca cggtaaagat gcgctgggtc aggtggatat
     2161 tgtcgccaac tacaacggtc gccgcttcca cggcgtcggc ctggccaccg atattgtcga
     2221 gtcctccgcc aaagccatgg tgcacgtact gaacaatatc tggcgtgccg cagaagtcga
     2281 aaaagagttg caacgcaaag ctcaacacaa cgaaaacaac aaggaaaccg tgtgatgtcg
     2341 aagaattacc atattgccgt attgccgggg gacggtattg gtccggaagt gatgacccag
     2401 gcgctgaaag tgctggatgc cgtgcgcaac cgctttgcga tgcgcatcac caccagccat
     2461 tacgatgtag gcggcgcagc cattgataac cacgggcaac cactgccgcc tgcgacggtt
     2521 gaaggttgtg agcaagccga tgccgtgctg tttggctcgg taggcggtcc gaaatgggaa
     2581 catttaccac cagaccagca accagaacgc ggcgcgctgt tgcctttgcg taagcacttc
     2641 aaattattca gcaacctgcg tccggcaaaa ctgtatcagg ggctggaagc attctgtccg
     2701 ctgcgtgctg acattgccgc taacggcttc gacatcctgt gcgtgcgcga actgaccggc
     2761 ggcatctatt tcggtcagcc aaaaggccgc gaaggtagcg gacagtatga aaaagcgttt
     2821 gataccgagg tgtatcaccg ttttgagatc gaacgtatcg cccgcatcgc gtttgaatct
     2881 gcccgcaagc gtcgccacaa agtcacctca atcgacaaag ccaacgtgct gcaatcctct
     2941 attttatggc gggagatcgt taacgagatc gccacggaat acccggatgt cgaactggcg
     3001 catatgtaca tcgacaacgc caccatgcag ctgattaaag atccatcaca gtttgacgtc
     3061 ctgctgtgct ccaacctgtt tggcgacatt ctgtctgacg agtgcgcaat gatcactggc
     3121 tcgatgggga tgttgccttc cgccagcctg aacgagcaag gttttggtct gtatgaaccg
     3181 gcaggcggct cagcaccaga tatcacaggc aaaaacatcg ccaacccgat tgcgcaaatt
     3241 ctgtcgctgg cactgctgct gcgctacagc ctggatgccg atgatgcggc ttgcgccatt
     3301 gaacgcgcca ttaaccgcgc attagaagaa ggcattcgca ccggggattt agcccgtggc
     3361 gctgccgccg ttagtaccga tgaaatgggc gatatcattg cccgctatgt ggcagaaggg
     3421 gtgtaatcat ggctaaaacg ttatacgaaa aattattcga cgctcacgta gtgtacgaag
     3481 ccgaaaacga aaccccgctg ttatatatcg accgccacct ggtgcatgaa gtgacctcac
     3541 cgcaggcgtt cgatggtctg cgcgcccacg gtcgcccggt acgtcagccg ggcaaaacct
     3601 tcgccactat ggatcacaac gtctctaccc agacgaaaga cattaatgcc tgtggtgaaa
     3661 tggcgcgcat ccagatgcag gagctgatta aaaactgcaa agaatttggc gtcgaactgt
     3721 atgacctgaa tcacccgtat caggggatcg tccacgtaat ggggccggaa cagggcgtca
     3781 ccttgccggg gatgaccatt gtctgcggcg actcgcatac cgccacccac ggcgcgtttg
     3841 gcgcactggc ctttggtatc ggcacttccg aagttgaaca cgtactggca acgcaaaccc
     3901 tgaaacaggg ccgcgcaaaa accatgaaaa ttgaagtcca gggcaaagcc gcgccgggca
     3961 ttaccgcaaa agatatcgtg ctggcaatta tcggtaaaac cggtagcgca ggcggcaccg
     4021 ggcatgtggt ggagttttgc ggcgaagcaa tccgtgattt aagcatggaa ggtcgtatga
     4081 ccctgtgcaa tatggcaatc gaaatgggcg caaaagccgg tctggttgca ccggacgaaa
     4141 ccacctttaa ctatgtcaaa ggccgtttgc atgcgccgaa aggcaaagat ttcgacgacg
     4201 ccgttgccta ctggaaaacc ctgcaaaccg acgaaggcgc aactttcgat accgttgtca
     4261 ctctgcaagc agaagaaatt tcaccgcagg tcacctgggg caccaatcca ggccaggtga
     4321 tttccgtgaa cgacaatatt cccgatccgg cttcgtttgc cgatccggtt gaacgcgcgt
     4381 cggcagaaaa agcgctggcc tatatggggc tgaaaccggg cattccgctg accgaagtgg
     4441 ctatcgacaa agtgtttatc ggttcctgta ccaactcgcg cattgaagat ttacgcgcgg
     4501 cagcggagat cgccaaaggg cgaaaagtcg cgccaggcgt gcaggcactg gtggttcccg
     4561 gctctggcca ggtaaaagcc caggcggaag cggaaggtct ggataaaatc tttattgaag
     4621 ccggttttga atggcgcttg cctggctgct caatgtgtct ggcgatgaac aacgaccgtc
     4681 tgaatccggg cgaacgttgt gcatccacca gcaaccgtaa ctttgaaggc cgccaggggc
     4741 gcggcgggcg cacgcatctg gtcagcccgg caatggctgc cgctgctgct gtgaccggac
     4801 atttcgccga cattcgcaac attaaataag gagcagacca tggcagagaa atttatcaaa
     4861 cacacaggcc tggtggttcc gctggatgcc gccaatgtcg ataccgatgc aatcatcccg
     4921 aaacagtttt tgcagaaagt gacccgtacg ggttttggcg cgcatctgtt taacgactgg
     4981 cgttttctcg atgaaaaagg ccaacagcca aacccggact tcgtgctgaa cttcccgcag
     5041 tatcagggcg cttccatttt gctggcacga gaaaacttcg gctgtggctc ctcgcgtgag
     5101 cacgcgccct gggcattgac cgactacggt tttaaagtgg tgattgcgcc gagttttgct
     5161 gacatcttct acggcaatag ctttaacaac cagctgctgc cggtgaaatt aagcgatgca
     5221 gaagtggacg aactgtttgc gctggtgaaa gccaatccgg ggatccattt cgacgtggat
     5281 ctggaagcgc aagaggtgaa agcgggagag aaaacctatc gctttaccat cgatgccttc
     5341 cgccgccact gcatgatgaa cggtctggac agtattgggc ttaccttgca gcacgacgac
     5401 gccattgccg cttatgaagc aaaacaacct gcgtttatga attaatcccc ttgcccggtc
     5461 aaatgaccgg gctttccgct atcgtccacg tcatcaaacg tctttaacct ttgcggttaa
     5521 aaataatgcg acaacagaaa taaccgcaat tacccagtag acagcaaagt gcccccaact
     5581 ttgtgcaatt gctccctgaa taacgccagc cagaattacc ccggtagaaa tactgttagt
     5641 aaataaggtg gtagctgccc ccgctcttcc aggcattaaa tcctgaaacc atagcatccc
     5701 aatacccgca acaatgccga taaatacagc gttaaaaagt tgcagcgtca tcaacgccat
     5761 acggctatga aagaaaatca atccggtgta aaacagtact cctgccgcca ctgctatgac
     5821 catcattcgc cgcttaccat aacgtttgac atagtagcca gccagaatca ttgctggtat
     5881 ttccagtcca gctgccgtcc ccatcaggaa acccgccagt ttgtctggca atcctaactc
     5941 gctactgatc cacaacggca tatcaataat gtacatggtg ttgcaggtcc acattaacgt
     6001 cgaggcgaca aataacatcc gtacgttact atcctgccag ccaccttgca ttgataaagc
     6061 attttccgac ggcagttcta cccgcgccac agacggaagc ataaatgcaa tcaataccag
     6121 actgagtgtg aatatcccgg cggcaatcga aaacatcacc acttctcgcg ccgagttatc
     6181 cgcatattcc cgcgccagag caaataactg tggcattgcc gtattggcca gagatgccag
     6241 aagcacacca caggtgataa gcgtcagata atgacgatta aatgcaaaca atggcgcatt
     6301 gccgatagcc atcaaacagc aaaatataat cagttttcgc cgatcgccct gactgtcaga
     6361 acgttttgcc aaccagaggc ttacgccgat cccagcaata gtattcaccg tataaaagag
     6421 tccgatccag aaaggttgcg cgccaacctc acgactcaga aataagctca atgtaggagc
     6481 ctgtagcgcc ccggccaccc ccatcataaa agcgaccagc ataaatgccg cgtaaacacc
     6541 gttcatacgg cgagccatcg tcattatcca gatcatacgt tcccttttta gcgcggcgag
     6601 aataaaaaaa accagcaggt ataatctgct ggcgggtgat tttacaccaa tactcagtca
     6661 cacatgatgc aggcaagtca actttcagaa ttgcggtcat cccactgcat cagtccttca
     6721 agcatggtta atcgttgtgg gacgcttaac caacgcaacc agcacaactt cgctgtcgcg
     6781 gtaaaatagt gctgataaaa ctgacgcatg gggcacctcc ttgcttcatc gttaggaatt
     6841 attgacttaa tatagggaaa ataaaattgc tgtcttttgc gcaggagttc cccttttatg
     6901 ccatctgctc gtctgcaaca acagttcatc cgcctgtggc aatgctgcga gggtaaatcg
     6961 caggacacaa cgctcaacga actggcagcg ttattgagct gctcgcgtcg tcatatgcgt
     7021 accctgctca acaccatgca ggatcgcggc tggctgacgt gggaagcgga agtcgggcgc
     7081 ggtaaacgct cgcgtctgac attcctctat accgggctgg cgcttcagca acagcgggcg
     7141 gaagacctgc tggagcagga tcgtatcgac caactagtgc agttggttgg cgacaaagcg
     7201 actgtgcggc aaatgctggt ttctcatctg ggccgcagct tccgccaggg gcggcacatc
     7261 ctgcgcgtgc tctactatcg tccgttgcgt aatctgctac ctggcagcgc attgcgccgt
     7321 tccgaaaccc atattgcccg gcaaatcttc agttcgctaa cgcgcataaa tgaggaaaat
     7381 ggggaactgg aagcagacat cgcccaccac tggcagcaaa tatcaccgct tcactggcgt
     7441 ttctttttgc gtccaggagt ccattttcat catggtcgtg aactggaaat ggacgacgtg
     7501 atcgcctctt taaaacgaat caatacgctg ccgctctatt cgcatattgc ggacattgtg
     7561 tcgccgacgc cctggacgct ggatatccat ctcacgcaac cggaccgctg gttaccgtta
     7621 ctgctggggc aagttccggc gatgatcctg ccgcgcgaat gggaaaccct cagtaacttt
     7681 gccagccatc ccatcggcac cggtccgtat gcggtgatcc gcaacaccac caatcaactg
     7741 aaaattcagg cattcgatga cttcttcggt taccgggcat taatcgatga agttaacgtc
     7801 tgggttctgc cggaaattgc cgacgagcca gctggaggtc tgatgctaaa aggtccacag
     7861 ggtgaggaaa aagagattga aagccgcctg gaggaaggtt gctactattt actattcgac
     7921 agccgcaccc atcgcggagc gaatcagcaa gtcagggact gggtaaacta tgtgctttct
     7981 ccaactaatc tggtctattt cgctgaggaa cagtaccagc aactgtggtt cccggcttat
     8041 ggactgctcc cccgttggca ccatgcccgc accataaaga gcgaaaaacc ggctggcctg
     8101 gaaagcctca ccctgacctt ttatcaggat cacagtgagc atcgggtgat tgccgggatc
     8161 atgcagcaga ttctggcaag tcaccaggtc acgctggaaa tcaaagagat cagctacgat
     8221 cagtggcatg aaggagagat cgagagcgat atctggctta atagcgccaa ctttacgctg
     8281 ccgctggatt tttcgctgtt cgcgcacttg tgcgaggtgc cgctgctcca acactgtatt
     8341 ccgatcgact ggcaagccga cgccgcccgc tggcgcaatg gcgaaatgaa cctggcgaac
     8401 tggtgccagc aactggtcgc cagtaaagca atggtgccgc ttatccacca ctggctgatc
     8461 attcagggac aacgcagtat gcgcggcctg cgtatgaata ccctcggctg gttcgatttt
     8521 aaatcagcgt ggtttgcgcc gccggatcca tgattgctgg tagataacaa aatcactaca
     8581 ctaacgccgt tctcaacggg gtgccacgca aacgcgtgcg ctgagaaaat acccgtcgaa
     8641 cctgatccgg ataacgccgg cgaagggatt tgaggctcct tctcaagtcc tttgccactc
     8701 ttttttgagg tgcaaagtgt taaaaaaatg tcttcccctg ctgttgctgt gcacagcgcc
     8761 cgttttcgct aaacccgttc tgactgttta tacctacgat tccttcgccg ccgactgggg
     8821 gcctggtcca aagatcaaaa aagcttttga agccgactgt aactgcgaac tgaaactggt
     8881 ggcgctggaa gatggcgttt cgcttctcaa ccgtctacgg atggaaggca aaaatagtaa
     8941 agccgatgtg gtgctggggc tggataacaa tctgttagac gccgccagta aaaccggact
     9001 atttgccaaa agcggtgtgg cagcggatgc cgttaacgtt cccggcggct ggaataatga
     9061 cactttcgtc ccgtttgatt acggctattt cgccttcgtc tatgacaaga acaaactgaa
     9121 aaacccgccg caaagcctga aagaattggt tgagagcgat caaaactggc gggtgattta
     9181 tcaggatccg cgcaccagta cgccggggct gggcctgctg ttgtggatgc aaaaagtcta
     9241 tggcgatgac gccccacaag cctggcaaaa actggcgaag aaaaccgtca cggtcaccaa
     9301 aggctggagc gaagcctacg gcctgttttt aaaaggtgaa agcgatctgg tactgagtta
     9361 caccacctct ccggcttatc acattctcga agagaaaaaa gataactacg ccgccgcgaa
     9421 ctttagcgaa ggtcactatc tgcaggtgga agtcgccgcc cgcaccgctg ccagcaagca
     9481 gccggagctg gcgcaaaaat tcctccagtt tatggtttct ccggcttttc agaatgcgat
     9541 cccaaccggt aactggatgt atccggtggc aaatgtcacg ctgcctgccg ggtttgaaca
     9601 attgaccaaa ccggccacca cgctggagtt cacaccagct gaagtggcgg cacaacgtca
     9661 ggcatggatt agcggatggc aacgcgccgt cagccgttaa ttcccggctg gttaattcca
     9721 ggtgtaagcg ccgccacgct ggtggtagcg gttgcgctcg cggcgtttct cgccctgtgg
     9781 tggaacgcgc cgcagggtaa ctgggtggca gtctggcagg acagctacct gtggcatgtg
     9841 gtgcgcttct ccttctggca ggcgtttctc tcggcgctac tctctgtcgt acccgcgata
     9901 ttcctcgccc gcgccctcta tcgcaggcgc tttccgggtc ggctggcgct gttgcgtctg
     9961 tgcgcaatga ccttgatcct cccggtgctg gttgctgttt tcggcattct tagcgtctat
    10021 ggtcgccagg gctggctggc atcgctctac caatcgctcg gtctggagtg gaccttttcg
    10081 ccctacggcc tgcaaggtat tttgctggcg cacgtatttt ttaatctgcc gatggcgagc
    10141 cgcttattac tccaggcact ggaaaacatt cccgacgaac aacgtcagct tgccgcccag
    10201 cttgggatgc gcggctggca ttttttccgc ttcgtcgaat ggccgtggtt acggcgacaa
    10261 atcccgccgg ttgctgcact tatctttatg ctctgtttcg ccagcttcgc caccgtgcta
    10321 tcactgggcg gcggtccgca ggcgaccact atcgagctgg ctatttatca ggcgctgagt
    10381 tacgactacg atcctgcccg cgcggcgatg ctggcgctga tccagatggt gtgttgcctt
    10441 gggctggtac tgttgagtca gcgattgagt aaggccattg cgccaggcac cacgctgctg
    10501 caaggctggc gcgacccgga cgatcgtctg catagccgca tttgcgacac ggtgttaata
    10561 gtgctggcgt tgctgctgtt gttgccaccg ttgctggcgg tgatcgtcga tggggtaaat
    10621 cgccagttgc cggaagtgct ggcacaaccg gtgctgtggc aggcgctgtg gacctcgttg
    10681 cgtattgcgc tggcggcagg tgtattgtgc gtagtgctga ccatgatgct gctatggagc
    10741 agtcgcgaac ttcgggcacg gcagaaaatg ctggcgggtc aggcgctgga gatgagcggc
    10801 atgttgattc tcgccatgcc ggggattgtg ctggctaccg gcttcttttt actgctcaac
    10861 aacactatcg gcctgcaaca atctgctgac ggcattgtga ttttcaccaa tgcgttaatg
    10921 gcgatccctt atgcgctgaa agtactggaa aacccgatgc gcgatatcac cgcccgctac
    10981 agtatgttgt gtcagtcgct gggcattgaa ggctggtcgc gcttaaaagt ggtggagctg
    11041 cgcgccctga aacgtccact ggcgcaggcg ctggcctttg cctgcgtgct gtcgattggt
    11101 gattttggcg tggtggcgtt gttcggtaac gatgatttcc gtaccctgcc gttttatctc
    11161 taccagcaaa ttggctccta tcgcagccag gacggcgcgg tcaccgcgtt aattctgctg
    11221 ctactctgtt ttctgctgtt taccgtgatt gaaaaactac cggggcgaaa tgttaaaact
    11281 gactgatatc acctggcttt accaacattt gccgatgcgt tttagcttaa cggtggaacg
    11341 cggcgagcag gtggcgatcc tcgggccaag cggcgcaggt aaaagtaccc tgctgaattt
    11401 gattgccggt tttctgacgc cagccagcgg ttcgctgact atcgatggcg ttgatcacac
    11461 cacgacgccg ccgtcacgcc gtccggtgtc gatgctgttt caggagaaca acctgttcag
    11521 ccacctgacg gtcgcgcaga acatcgggct ggggcttaat ccaggattga agctgaacgc
    11581 ggcacagcag gagaaaatgc acgctatcgc ccgtcagatg gggattgata atttaatggc
    11641 gcggttaccg ggcgagcttt ccggcggcca gcgacagcgc gtggcgttag cgcgttgtct
    11701 ggtgcgcgaa cagccgattt tattgctcga tgaaccgttc tctgcgctcg atccggcgtt
    11761 acgtcaggag atgctgacgc tggtgagcac gagctgccag cagcaaaaaa tgacgctatt
    11821 gatggtgtcg cacagtgtgg aagatgcggc gcggatcgcc acacgctcgg tggtggtcgc
    11881 cgacgggcgc atcgcctggc agggtaagac tgaagaattg ttgagcggta aggcgagtgc
    11941 ttcggcaata ttggggatta cgggttagta tgcactgtca gatgcggcgt gaacacctta
    12001 tccgacctac ggacagtgcg ttttgtaggc ctgataagac cggtcagcgt cgcatcaggc
    12061 aacccgcaca agacaatccc ctaaacccca accactttac gcaaaatatc gatatacacc
    12121 ggcatcaacg ggtggcgaat taacaccacc agcgccacca cgccgatggc agaaatcaac
    12181 ggcgtcagcc acaacaaacg gccgcgcgac aaataatgac ttaaacgatc ggttgcttta
    12241 ccgctgcgcc ataaccgcca gcacagccag ccaccaaccc acaaaaatac cgccgtcgcc
    12301 agcagcaacc atttaaactc gccgctctgc attccggctg gaatatcgat cgccgcgccc
    12361 gccagaatcc caggcaggaa gtaaaacggt ggccacagca ggcagccgat aatattcggc
    12421 gtaatgaatt ttgccaccgg cagatccagc attcccgcca ccattggcac cagcggacgc
    12481 gtcgggccaa caaagcgacc gaccagaatg gtgaacatgc tgtgttgatg caacgcatgt
    12541 tcagttttat cgagtaatgc tttgtttttc ttcagaaatg accagcgatg caacggcttt
    12601 ttaaaacgcc agcccagcca gaaggagatc cagtcgccca gcagacaacc gacaatccct
    12661 gccagccagg cgtgccagaa acttaactcg ccgctgccaa tcagcgctcc cagccccgcc
    12721 atcagcaccg taccgggtag aatcaaaccg accagcgcca gcgactccag aaaggccacc
    12781 aacaccaccg ccatcaatga atacacggta gattgggtaa taaagtgttc cagcaatgct
    12841 tgcataatgt gcctgtcaaa tggacgaagc agggattctg caaaccctat gctactccgt
    12901 caagccgtca attgtctgat tcgttaccaa ttatgacaac ttgacggcta catcattcac
    12961 tttttcttca caaccggcac ggaactcgct cgggctggcc ccggtgcatt ttttaaatac
    13021 ccgcgagaaa tagagttgat cgtcaaaacc aacattgcga ccgacggtgg cgataggcat
    13081 ccgggtggtg ctcaaaagca gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa
    13141 gacgctaatc cctaactgct ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac
    13201 atgctgtgcg acgctggcga tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg
    13261 acaagcctcg cgtacccgat tatccatcgg tggatggagc tactcgttaa tcgcttccat
    13321 gcgccgcagt aacaattgct caagcagatt tatcgccagc agctccgaat agcgcccttc
    13381 cccttgcccg gcgttaatga tttgcccaaa caggtcgctg aaatgcggct ggtgcgcttc
    13441 atccgggcga aagaaacccg tattggcaaa tattgacggc cagttaagcc attcatgcca
    13501 gtaggcgcgc ggacgaaagt aaacccactg gtgataccat tcgcgagcct ccggatgacg
    13561 accgtagtga tgaatctctc ctggcgggaa cagcaaaata tcacccggtc ggcaaacaaa
    13621 ttctcgtccc tgatttttca ccaccccctg accgcgaatg gtgagattga gaatataacc
    13681 tttcattccc agcggtcggt cgataaaaaa atcgagataa ccgttggcct caatcggcgt
    13741 taaacccgcc accagatggg cattaaacga gtatcccggc agcaggggat cattttgcgc
    13801 ttcagccata cttttcatac tcccgccatt cagagaagaa accaattgtc catattgcat
    13861 cagacattgc cgtcactgcg tcttttactg gctcttctcg ctaacccaac cggtaacccc
    13921 gcttattaaa agcattctgt aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa
    13981 aagtgtctat aatcacggca gaaaagtcca cattgattat ttgcacggcg tcacactttg
    14041 ctattccata gcatttttat ccataagatt agcggatcct acctgacgct ttttatcgca
    14101 actctctact gtttctccat acccgttttt ttggatggag tgaaacgatg gcgattgcaa
    14161 ttggcctcga ttttggcagt gattctgtgc gagctttggc ggtggactgc gccagcggtg
    14221 aagagatcgc caccagcgta gagtggtatc cccgttggca aaaagggcaa ttttgtgatg
    14281 ccccgaataa ccagttccgt catcatccgc gtgactacat tgagtcaatg gaagcggcac
    14341 tgaaaaccgt gcttgcagag cttagcgtcg aacagcgcgc agctgtggtc gggattggcg
    14401 ttgacagtac cggctcgacg cccgcaccga ttgatgccga cggtaacgtg ctggcgctgc
    14461 gcccggagtt tgccgaaaac ccaaacgcga tgttcgtatt gtggaaagac cacactgcgg
    14521 ttgaagaagc ggaagagatt acccgtttgt gccacgcgcc gggcaacgtt gactactccc
    14581 gctacattgg tggtatttat tccagcgaat ggttctgggc aaaaatcctg catgtgactc
    14641 gccaggacag cgccgtggcg caatctgccg catcgtggat tgagctgtgc gactgggtgc
    14701 cagctctgct ttccggtacc acccgcccgc aggatattcg tcgcggacgt tgcagcgccg
    14761 ggcataaatc tctgtggcac gaaagctggg gcggcttgcc gccagccagt ttctttgatg
    14821 agctggaccc gatcctcaat cgccatttgc cttccccgct gttcactgac acctggactg
    14881 ccgatattcc ggtgggcacc ttatgcccgg aatgggcgca gcgtctcggc ctgcctgaaa
    14941 gcgtggtgat ttccggcggc gcgtttgact gccatatggg cgcagttggc gcaggcgcac
    15001 agcctaacgc actggtaaaa gttatcggta cttccacctg cgacattctg attgccgaca
    15061 aacagagcgt tggcgagcgg gcagttaaag gtatttgcgg tcaggttgat ggcagcgtgg
    15121 tacctggatt tatcggtctg gaagcaggcc aatcggcgtt tggtgatatc tacgcctggt
    15181 ttgatcgcgt actcggctgg ccgctggaac agcttgccgc ccagcatccg gaactgaaag
    15241 cgcaaatcaa cgccagccag aaacaactgc ttccggcgct gaccgaagca tgggccaaaa
    15301 atccgtctct gaatcacctg ccggtggtgc tcaactggtt taacggccgc cgcacaccga
    15361 acgctaacca acgcctgaaa ggggtgatta ccgatcttaa cctcgctacc gacgctccgc
    15421 tgctgtttgg cggtttgatt gctgccaccg cctttggcgc acgcgcaatc atggagtgct
    15481 ttaccgatca ggggatcgcc gtcaataacg tgatggcgct gggcggcatc gcgcggaaaa
    15541 accaggtcat tatgcaggcc tgctgcgacg tgctgaatcg cccgctgcaa attgttgcct
    15601 ctgaccagtg ctgtgcgctc ggtgcggcga tttttgctgc cgtcgccgcg aaagtgcacg
    15661 cagacatccc atcagcccag caaaaaatgg ccagtgcggt agagaaaacc ctgcaaccgc
    15721 gcagcgaaca gacacaacgc tttgaacagc tttatcgccg ctatcagcaa tgggcgatga
    15781 gcgccgaaca acactatctt ccaacttccg ccccggcaca ggctgcccag gccgttccga
    15841 ctctataagg acacgataat gacgattttt gataattatg aagtgtggtt tgtcattggc
    15901 agccagcatc tgtatggccc ggaaaccctg cgtcaggtca cccaacatgc cgagcacgtt
    15961 gttaatgcgc tgaatacgga agcgaaactg ccctgcaaac tggtgttgaa accgctgggc
    16021 accacgccgg atgaaatcac cgctatttgc cgcgacgcga attacgacga tcgttgcgct
    16081 ggtctggtgg tgtggctgca caccttctcc ccggccaaaa tgtggatcaa cggcctgacc
    16141 atgctcaaca aaccgttgct gcaattccac acccagttta acgcggcgct gccgtgggac
    16201 agtatcgata tggactttat gaacctgaac cagactgcac atggcggtcg cgagttcggc
    16261 ttcattggcg cgcgtatgcg tcagcaacat gccgtggtta ccggtcactg gcaggataaa
    16321 caagcccatg agcgtatcgg ctcctggatg cgtcaggcgg tctctaaaca ggatacccgt
    16381 catctgaaag tctgccgttt tggcgataac atgcgtgaag tagcggtcac cgatggtgat
    16441 aaagttgccg cacagatcaa gttcggtttc tccgtcaata cctgggcggt tggcgatctg
    16501 gtgcaggtgg tgaactccat cagcgacggc gatgttaacg cgctggtcga tgagtacgaa
    16561 agctgctaca ccatgacgcc tgccacacaa atccacggcg aaaaacgaca gaacgtgctg
    16621 gaagcggcgc gtattgagct ggggatgaag cgtttcctgg aacaaggtgg cttccacgcg
    16681 ttcaccacca cctttgaaga tttgcacggt ctgaaacagc ttccaggtct ggccgtacag
    16741 cgtctgatgc agcagggtta cggctttgcg ggcgaaggcg actggaaaac cgccgccctg
    16801 cttcgcatca tgaaggtgat gtcaaccggt ctgcagggcg gcacctcctt tatggaggac
    16861 tacacctatc acttcgagaa aggtaatgac ctggtgctcg gctcccatat gctggaagtc
    16921 tgcccgtcga ttgccgtaga agagaaaccg atcctcgacg ttcagcatct cggtattggt
    16981 ggtaaggacg atcctgcccg actgatcttc aatacccaaa ccggtccagc gattgtcgcc
    17041 agcctgattg atctcggcga tcgttaccgt ctgctggtta actgtatcga cacggtgaaa
    17101 acaccgcact ccctgccgaa actgccggtg gcgaatgcgc tgtggaaagc gcaaccggat
    17161 ctgccaactg cttccgaagc gtggatcctc gctggtggcg cgcaccatac cgtcttcagc
    17221 catgcgctga acctcaacga tatgcgtcag ttcgccgaga tgcacgacat tgaaatcacg
    17281 gtgattgata acgacacccg cctgccagcg tttaaagacg cactgcgctg gaacgaagtg
    17341 tattacggat ttcgtcgcta agtagccaca tcaggtatgt aacgcctgat gcgacgctga
    17401 cgcgtcttat ctggcctaca cgctgcgatt ttgtaggccg gataagcaaa gcgcatccgg
    17461 cattcaacgc ctgatgcgac gctggcgcgt cttatcaggc ctacgcgctg cgattttgta
    17521 gaccggataa gcgaagcgca tccggcacga aggagtcaac atgttagaag atctcaaacg
    17581 ccaggtatta gaagccaacc tggcgctgcc aaaacacaac ctggtcacgc tcacatgggg
    17641 caacgtcagc gccgttgatc gcgagcgcgg cgtctttgtg atcaaacctt ccggcgtcga
    17701 ttacagcgtc atgaccgctg acgatatggt cgtggttagc atcgaaacag gtgaagtggt
    17761 tgaaggtacg aaaaagccct cctccgacac gccaactcac cggctgctct atcaggcatt
    17821 cccctccatt ggcggcattg tgcatacgca ctcgcgccac gccaccatct gggcgcagtc
    17881 gggtcagtcg attccagcaa ccggcaccac ccacgccgac tatttctacg gcaccattcc
    17941 ctgcacccgc aaaatgaccg acgcagaaat caacggcgaa tatgagtggg aaaccggtaa
    18001 cgtcatcgta gaaacctttg aaaaacaggg tatcgatgca gcgcaaatgc ccggcgttct
    18061 ggtccattcc cacggcccgt ttgcatgggg caaaaatgcc gaagatgcgg tgcataacgc
    18121 catcgtgctg gaagaggtcg cttatatgga gatattctgc cgtcagttag cgccgcagtt
    18181 accggatatg cagcaaacgc tgctggataa acactatctg cgtaagcatg gcgcgaaggc
    18241 atattacggg cagtaatgac tgtataaaac cacagccaat caaacgaaac caggctatac
    18301 tcaagcctgg ttttttgatg gattttcagc gtggcgcagg caggttttat cttaacccga
    18361 cactggcggg acaccccgca agggacagaa gtctccttct ggctggcgac ggacaacggg
    18421 ccgttgcagg ttacgcttgc accgcaagag tccgtggcgt ttattcccgc cgatcaggtt
    18481 ccccgcgctc agcatatttt gcagggtgaa caaggctttc gcctgacacc gctggcgtta
    18541 aaggattttc accgccagcc ggtgtatggc ctttactgtc gcgcccatcg ccaattgatg
    18601 aattacgaaa agcgcctgcg tgaaggtggc gttaccgtct acgaggccga tgtgcgcccg
    18661 ccagaacgct atctgatgga gcggtttatc acctcaccgg tgtgggtcga gggtgatatg
    18721 cacaatggca ctatcgttaa tgcccgtctg aaaccgcatc ccgactatcg tccgccgctc
    18781 aagtgggttt ctatagatat tgaaaccacc cgccacggtg agctgtactg catcggcctg
    18841 gaaggctgcg ggcagcgcat cgtttatatg ctggggtcgg agaatggcga cgcctcctcg
    18901 cttgatttcg aactggaata cgtcgccagc cgcccgcagt tgctggaaaa actcaacgcc
    18961 tggtttgcca actacgatcc tgatgtgatc atcggttgga acgtggtgca gttcgatctg
    19021 cgaatgctgc aaaaacatgc cgagcgttac cgtcttccgc tgcgtcttgg gcgcgataat
    19081 agcgagctgg agtggcgcga gcacggcttt aaaaacggcg tcttttttgc ccaggctaaa
    19141 ggtcggctaa ttatcgacgg tatcgaggcg ctgaaatccg cgttctggaa tttctcttca
    19201 ttctcgctgg aaactgtcgc tcaggagcta ttaggcgaag gaaaatctat cgataacccg
    19261 tgggatcgaa tggacgaaat tgaccgccgt ttcgccgaag ataaacctgc gctggcaact
    19321 tataacctga aagattgcga gctggtgacg cagatcttcc acaaaactga aatcatgcca
    19381 tttttactcg aacgggcaac ggtgaacggc ctgccggtgg accgacacgg cggttcggtg
    19441 gcggcatttg gtcatctcta ttttccgcga atgcatcgcg ctggttatgt cgcgcctaat
    19501 ctcggcgaag tgccgccgca cgccagccct ggcggctacg tgatggattc acggccaggg
    19561 ctttatgatt cagtgctggt gctggactat aaaagcctgt acccgtcgat catccgcacc
    19621 tttctgattg atcccgtcgg gctggtggaa ggcatggcgc agcctgatcc agagcacagt
    19681 accgaaggtt ttctcgatgc ctggttctcg cgagaaaaac attgcctgcc ggagattgtg
    19741 actaacatct ggcacgggcg cgatgaagcc aaacgccagg gtaacaaacc gctgtcgcag
    19801 gcgctgaaaa tcatcatgaa tgccttttat ggcgtgctcg gcaccaccgc ctgccgcttc
    19861 ttcgatccgc ggctggcatc gtcgatcacc atgcgtggtc atcagatcat gcggcaaacc
    19921 aaagcgttga ttgaagcaca gggctacgac gttatctacg gcgataccga ctcaacgttt
    19981 gtctggctga aaggcgcaca ttcggaagaa gaagcggcga aaatcggtcg tgcactggtg
    20041 cagcacgtta acgcctggtg ggcggaaacg ctgcaaaaac aacggctgac cagcgcatta
    20101 gaactggagt atgaaaccca tttctgccgt tttctgatgc caaccattcg cggagccgat
    20161 accggcagta aaaagcgcta cgccgggctg attcaggagg gcgataaaca gcggatggtg
    20221 tttaaagggc tggaaaccgt gcgcaccgac tggacgccgc tggcccagca gtttcagcag
    20281 gagctatacc tgcgcatctt ccgcaacgag ccatatcagg aatatgtacg cgaaaccatc
    20341 gacaaactga tggcgggtga actggatgcg cgactggttt accgtaaacg ccttcgccgt
    20401 ccgctgagcg agtatcagcg taatgtgccg cctcatgtac gcgccgctcg ccttgccgat
    20461 gaagaaaacc aaaagcgtgg tcgccccttg caatatcaga atcgcggcac cattaagtac
    20521 gtatggacca ccaacggccc ggagccgctg gactaccaac gttcaccact ggattacgaa
    20581 cactatctga cccgccagct acaacccgtg gcggagggaa tactcccttt tattgaggat
    20641 aattttgcta cacttatgac cgggcaactt gggctatttt gagcaaaaaa agagttcgcc
    20701 agataccatt ttgatgcgtg acgaatgctt tgccatccag taccatagcg ccctttccat
    20761 tcctggacct gaataacacc actacctcat aagcacggta gcgggtggtt attgcctgca
    20821 attaaagata tagagccgaa cacatatgcc ttttacactt ggtcaacgct ggatcagcga
    20881 tacagaaagc gaattgggac ttggaaccgt tgtcgcggtg gatgcgcgaa ctgtcacttt
    20941 acttttccca tctactggtg aaaaccgtct gtacgcacgc agtgattccc ccgtgacccg
    21001 cgtgatgttc aaccctggtg ataccattac cagccatgac ggctggcaga tgcaagtcga
    21061 agaagtaaaa gaagaaaatg gcttgctgac ctatatcggt actcgcctgg atactgaaga
    21121 gtccggcgta gccctgcgtg aagttttcct tgatagcaaa ctggtgttca gcaaaccgca
    21181 ggaccgcctg tttgccgggc agattgaccg tatggaccgc tttgcgctgc gttatcgcgc
    21241 gcgtaagtat tccagcgaac agttccgtat gccgtacagc ggcctgcgcg gtcagcgtac
    21301 cagcctgatc ccgcaccagc tcaacatcgc tcatgatgtt ggccgccgcc acgcgccgcg
    21361 cgtcctgctg gctgacgaag tgggtttagg gaaaaccatt gaagccggga tgatcctgca
    21421 tcagcaactg ctctctggcg ctgctgaacg tgtgctgatt atcgtcccgg aaaccttaca
    21481 gcatcagtgg ctggtagaaa tgctgcgccg tttcaacctg cgctttgctc tgtttgatga
    21541 tgagcgttat gccgaagctc agcacgatgc ttacaacccg ttcgacaccg aacagctggt
    21601 gatttgctcg ctggattttg cccgtcgtag caaacagcgc ctggaacatc tctgtgaagc
    21661 cgaatgggac ctgctggtgg tcgatgaagc gcatcacctg gtgtggagcg aagatgcgcc
    21721 aagccgtgaa tatcaggcca ttgaacaact ggcagagcac atgccgggcg ttctgctgct
    21781 gaccgcgacc ccggaacagc tggggatgga aagccacttc gcccgtctgc gtctgctgga
    21841 cccgaaccgt ttccacgatt ttgcgcagtt cgttgaagag cagaaaaatt atcgtccggt
    21901 tgcggacgcc gttgccatgc tgctggcagg taacaaactg agcaatgacg aactgaacat
    21961 gctcggcgag atgatcggcg agcaggatat cgagtcgctg ttgcaggcag caaacagcga
    22021 cagcgaagat gcccagagcg cccgtcagga gctggtttcg atgctgatgg atcgccacgg
    22081 caccagccgc gtgctgttcc gtaacacgcg taacggtgtg aaaggattcc cgaaacgcga
    22141 gctgcacacc attaagctgc cgctaccgac gcagtatcag acggctatta aagtctccgg
    22201 cattatgggc gcacgtaaaa gtgcggaaga tcgtgctcgc gatatgctct acccggagcg
    22261 tatttatcag gaatttgaag gtgataacgc cacctggtgg aacttcgatc cgcgcgttga
    22321 gtggctgatg ggctacctga ccagccatcg ctctcagaaa gtgctggtga tctgcgccaa
    22381 agctgccact gcgctgcaac tggagcaggt actgcgcgaa cgtgaaggta ttcgcgctgc
    22441 ggtgttccac gaaggtatgt cgattatcga acgtgaccgc gctgccgcct ggtttgccga
    22501 agaagacacc ggcgcacagg tactgctgtg ctcagaaatc ggttctgaag gacgtaactt
    22561 ccagttcgcc agccacatgg tgatgtttga cctgccattc aacccggatc tactggagca
    22621 gcgtattggt cgtctggatc gtatcggcca ggcgcacgat attcagatcc atgtgcctta
    22681 tctggagaaa accgctcagt cggtgctggt gcgctggtat cacgaaggtc tggatgcatt
    22741 tgagcacacc tgcccgaccg gacgcactat ttacgatagc gtatacaacg atctgattaa
    22801 ctatctggct tcaccggatc aaaccgaagg ctttgacgat ctgatcaaaa actgccgcga
    22861 gcaacatgaa gcgctgaaag cacagctgga acagggtcgt gaccgcctgc tggaaatcca
    22921 ctccaacggt ggcgaaaaag cccaggcact ggcagaaagc attgaagagc aggatgacga
    22981 taccaacctg atcgccttcg ccatgaacct gctcgatatt atcggtatca atcaggacga
    23041 tcgcggcgac aacatgatcg tgctgacgcc gtccgatcat atgctggtgc cggacttccc
    23101 tggcctgtcg gaagatggca tcaccatcac ctttgatcgt gaagtggcgc tggcgcgtga
    23161 agatgcacag tttattacct gggagcatcc gctgatccgc aacggtctgg atctgatcct
    23221 ttctggcgat accggtagca gcacgatttc actgttaaaa aacaaagcgt tgccggtagg
    23281 tacgctgttg gtggaactga tttatgtggt tgaagcccag gctccgaagc agttgcagct
    23341 caaccgcttc ctgccaccga cgccggtacg tatgctgctg gataaaaacg gcaacaacct
    23401 ggcggcgcag gtagagtttg aaacctttaa ccgccagctt aacgcggtta accgtcacac
    23461 cggcagcaaa ctggttaacg ccgtgcagca ggatgttcac gctatccttc aactgggtga
    23521 agcgcagatc gagaaatctg cccgtgcatt gattgatgca gcgcgtaacg aagccgacga
    23581 aaaactgtct gccgagctgt ctcgtctgga agctctgcgt gcagtgaacc cgaacattcg
    23641 tgacgacgaa ctgaccgcca ttgagagcaa ccgtcagcag gtaatggaaa gcctggatca
    23701 ggcaggttgg cgtctggatg ccctgcgttt gatcgttgta acgcatcagt aacggagccg
    23761 aaaatgggga tggaaaacta caatccaccg caggaaccct ggttggttat cctgtatcag
    23821 gatgaccaca ttatggtggt taacaagccg agcggtttgt tgtctgtgcc ggggcgtctg
    23881 gaagagcaca aagacagcgt gatgacgcgc attcagcgtg attatccgca ggcagaatcg
    23941 gtgcatcgtc tggatatggc taccagcggc gtgattgtag tggcgctgac caaagccgcg
    24001 gagcgggagt taaaacgcca gttccgcgag cgcgagccga aaaagcagta tgtggcccgc
    24061 gtctgggggc atccatcccc tgcggaaggt ctggtggatc tgccgctgat ttgcgactgg
    24121 ccaaaccgcc cgaaacagaa agtctgttac gaaacgggta aacctgcgca gacggaatat
    24181 gaagtggtgg agtatgcggc ggataacacg gcaagagtgg tgttaaaacc gattactggg
    24241 cgttcgcatc aattgcgtgt gcatatgctg gcgctggggc atccgattct cggcgatcgt
    24301 ttttatgcat caccagaagc gagagcgatg gcaccgcgtt tgttgctaca tgcagagatg
    24361 ttgacgatta cccatccggc gtatggcaat agtatgacgt ttaaagcgcc agcggatttt
    24421 taatagtcac gctggcgcag ttcggtaggc ggataaggcg ttcacgccga atccggcaat
    24481 tgtgcgccga ttgcctgatg cgacgcgggc cgcgtcttat caggcctaca gtttaaaact
    24541 tatttaaacc ctttctgctg ctttatcagc tcatatgcct gctgaatttc ctgcgctttc
    24601 tgcttcgcca tctccatcat ctcaggcggc aaacctttcg ccaccagctt atcgggatgg
    24661 tgttcactca tcagcttacg gtaggctcgt ttaatggtgg tcgcatcatc cgtcggcttc
    24721 acacccagca cattacaggc atcttccagc gtcgggccac gctgcgcttg ctgccagtta
    24781 ccaccgccag attgctgctg ataaccgccg ccaaactgtg caccgccctg catcatgcgc
    24841 aaaaactggt caaactgggc gcgggagatc cctaattctt ctgcaatgac atacagcacc
    24901 gcccgttcat tcgggtgcag tgaaccatca gcaaacgccg cctgaatctg gatctccaga
    24961 aacatacgaa ttaagtcaaa acgaccaaag cagacactgc gaaactggcg catcttttcg
    25021 cgcagcgggt aattgtctga ttttcccacc cggaacgcgt tttgcgccgc agtacgggaa
    25081 gcgccatgaa ggttcattcg gtccatcagc tggctggcga tatgaatatc agcctccgtg
    25141 acgcgacctt tggatttggt taaatgcccc atcacttcaa aagtggtggc aaaaaacagc
    25201 gcctgacgct cacgctggtt ggcgaaccac gccattttac ggctacgggc tttatcaaac
    25261 atatggccaa ttaacaggcc taacactacg ccccaaaagc cgccgcccat cagtaaggcc
    25321 acggccacgc caatgatttt tccccaatac tgcatatatt ccccaaatcg acacacggat
    25381 atcagggcta tctcccacaa tataaaggtg cttttaccgt tttccggctg cagtcaattg
    25441 tgggacatcg cctataattt gcattatcat acctgtcatt caatgccgtg cctaacacca
    25501 ctgacgctat tcggacagga ttaacactag cgtagagatg acgagtacgt tagtctctga
    25561 ccgtttgtca cgcgcaacgt taccgatgat ggaacaataa aatcaacgta tgaaaaaacg
    25621 tatccccact ctcctggcca ccatgattgc caccgccctt tatagtcaac agggactggc
    25681 agccgacctc gcctcacagt gcatgttggg cgtgccaagc tatgaccgtc ctctggtaca
    25741 gggcgatacc aatgacttac ccgtgactat caatgctgac cacgcgaaag gggactaccc
    25801 ggatgacgcc gtgtttactg gcagcgtgga tatcatgcag ggtaacagcc gtctgcaggc
    25861 cgacgaagtg cagctccatc aaaaagaggc accaggacaa ccggagccgg tacgaaccgt
    25921 tgatgcgctc ggtaatgtcc attacgacga taaccaggtg atcctcaaag ggccgaaagg
    25981 ctgggcgaat ctgaacacca aagataccaa cgtctgggaa ggtgattacc agatggtggg
    26041 tcgccagggc cgcggtaaag cggacctgat gaaacaacgt ggtgaaaacc gctataccat
    26101 tctggataac ggtagcttta cctcctgtct gccgggttct gacacctgga gcgtggtagg
    26161 tagcgaaatt attcatgacc gcgaagaaca agttgcggag atctggaacg cccgctttaa
    26221 ggtgggtcca gtaccgatct tttatagccc ctatttgcag ttgccggtgg gtgacaaacg
    26281 tcgctctggt ttcttgatcc cgaacgccaa gtacaccacc accaactact ttgagttcta
    26341 cctgccatat tactggaaca tcgcgccaaa tatggatgcc accatcacgc cgcattatat
    26401 gcatcgtcgt ggcaacatca tgtgggagaa cgaattccgc tacctctccc aggcgggcgc
    26461 tggcttgatg gaactggact atctgccttc agataaagtc tatgaagatg aacacccgaa
    26521 cgatgacagt tcacgtcgtt ggttgttcta ctggcaacac tccggggtca tggatcaagt
    26581 gtggcgtttc aacgtagact acaccaaggt cagcgatcct agctacttca atgatttcga
    26641 taacaagtac ggttccagta ctgacggcta cgcaacgcaa aaattcagcg ttggctatgc
    26701 ggtgcaaaac ttcaatgcca ccgtttcaac caagcagttc caggtcttta gcgagcagaa
    26761 caccagtagc tactcggcag agccgcagtt agacgttaac tactaccaga atgatgttgg
    26821 tccgtttgat acgcgtattt acggccaggc agtgcacttt gttaacacca gagacgacat
    26881 gcctgaagca acccgtgttc acctggaacc gaccatcaat ttgccgctct ctaataactg
    26941 gggcagcatc aataccgaag cgaagttgct ggcaacccat tatcagcaaa ccaatcttga
    27001 ctggtataac tccagaaaca cgaccaagct ggacgaatcc gttaaccgcg ttatgccgca
    27061 attcaaagtt gacggcaaaa tggtctttga acgcgatatg gaaatgctgg ctccgggtta
    27121 tacccaaacg ctggaaccgc gcgcgcagta tttgtacgtg ccgtatcgcg atcagagcga
    27181 catctataac tacgactcgt ctctactgca atctgactac tctggcctgt tccgggaccg
    27241 gacttacggc ggtcttgacc gtattgcctc cgctaaccag gtgacgaccg gtgtcacatc
    27301 tcgcatatat gatgatgctg ccgttgaaca ttttaatatt tccgttggtc aaatctacta
    27361 tttcacggag tctcgcactg gcgatgacaa cataacatgg gagaatgacg acaaaacggg
    27421 ctcactggtg tgggcaggcg atacttactg gcgtatctcc gagcgttggg gattgcgtgg
    27481 cgggattcag tacgatacac gtctggataa cgtagcgacc agtaactcca gcattgaata
    27541 ccgtcgggat gaagaccgtc tggtacagct gaattaccgt tacgccagcc cggaatatat
    27601 tcaggctacg ctgcctaagt actattccac tgctgagcaa tataagaatg gtatttcgca
    27661 ggtaggtgct gtcgccagct ggccaattgc cgatcgttgg tccattgttg gggcctacta
    27721 ctacgacacc aatgcgaaca agcaagccga ctctatgtta ggtgtgcaat acagctcctg
    27781 ctgctatgca attcgcgtcg gttacgagcg gaagctgaac ggttgggata acgataaaca
    27841 acatgcggta tatgacaacg caatcggctt taacatcgaa cttcgcggcc tgagctccaa
    27901 ctacggtctg ggtacgcaag agatgctgcg ttcgaacatt ctgccgtatc aaaacacttt
    27961 gtgatttgtt gatttaccac gtaatccgca gtgcggttaa ttgaaatgga aaaagtatga
    28021 agaactggaa aacgctgctt ctcggtatcg ccatgatcgc gaataccagt ttcgctgccc
    28081 cccaggtagt cgataaagtc gcagccgtcg tcaataacgg cgtcgtgctg gaaagcgacg
    28141 ttgatggatt aatgcagtcg gtaaaactga acgctgcgca ggcaaggcag cagcttcctg
    28201 atgacgcgac gctgcgccac caaatcatgg aacgtttgat catggatcaa atcatcctgc
    28261 agatggggca gaaaatggga gtgaaaatct ccgatgagca gctggatcag gcgattgcta
    28321 acatcgcgaa acagaacaac atgacgctgg atcagatgcg cagccgtctg gcttacgatg
    28381 gtctgaacta caacacctat cgtaaccaga tccgcaaaga gatgattatc tctgaagtgc
    28441 gtaacaacga ggtgcgtcgt cgcatcacca tcctgccgca ggaagtcgaa tccctggcgc
    28501 agcaggtggg taaccaaaac gacgccagca ctgagctgaa cctgagccac atcctgatcc
    28561 cgctgccgga aaacccgact tctgatcagg tgaacgaagc ggaaagccag gcgcgcgcca
    28621 ttgtcgatca ggcgcgtaac ggcgctgatt tcggtaagct ggcgattgct cattctgccg
    28681 accagcaggc gctgaacggc ggccagatgg gctggggccg tattcaggag ttgccgggta
    28741 tcttcgccca ggcattaagc accgcgaaga aaggcgacat tgttggcccg attcgttccg
    28801 gcgttggctt ccatattctg aaagttaacg acctgcgcgg cgaaagcaaa aatatctcgg
    28861 tgaccgaagt tcatgctcgc catattctgc tgaaaccgtc gccgatcatg actgacgaac
    28921 aggcccgtgt gaaactggaa cagattgctg ccgatatcaa gagtggtaaa acgacttttg
    28981 ctgccgcagc gaaagagttc tctcaggatc caggctctgc taaccagggc ggtgatctcg
    29041 gctgggctac accagatatt ttcgatccgg ccttccgtga cgccctgacc cgcctgaaca
    29101 aaggtcaaat gagtgcaccg gttcactctt cattcggctg gcatttaatc gaactgctgg
    29161 atacccgtaa tgtcgataaa accgacgctg cgcagaagga tcgtgcatac cgcatgctga
    29221 tgaaccgtaa gttctcggaa gaagcagcaa gctggatgca ggaacaacgc gccagcgcct
    29281 acgttaaaat cctgagcaac taatggttaa aacccaacgt gttgtgatca ctcccggcga
    29341 gcccgccggg attggcccgg acttagttgt ccagcttgca cagcgtgagt ggccggtcga
    29401 actggttgtt tgtgccgatg ccactctcct taccgaccgg gcagcgatgc tcggtttgcc
    29461 gctcaccctc cgcccttatt cccccaactc ccctgcacaa ccgcaaactg cgggcacatt
    29521 aacgctactt cctgtcgcgc tacgcgaatc tgtcactgcg gggcagttag cgattgaaaa
    29581 tgggcattac gtggtggaga cgctggcgcg agcgtgtgat ggctgtctga acggtgaatt
    29641 tgctgcgctg atcacaggcc ccgtgcataa aggcgtcatt aacgacgcag gtattccgtt
    29701 tactggtcat accgagtttt tcgaagagcg ctcgcaggcg aaaaaagtgg tgatgatgct
    29761 ggcgaccgaa gaacttcgcg tggcgctggc aacgacgcat ttaccgctgc gcgatatcgc
    29821 agatgctatc acccctgcac ttttgcacga agtgattgct attttgcatc acgatttgcg
    29881 tacaaaattt ggtattgccg aaccgcgcat tctggtctgc gggctgaatc cgcacgcggg
    29941 cgaaggcggt catatgggta cggaagagat agacaccatt attccggtgc tcgacgagct
    30001 gcgggcgcag gggatgatac tcaacgggcc gctgcctgcc gataccctgt ttcagccgaa
    30061 atatctcgat aacgccgacg ccgtgctggc gatgtaccac gatcagggtc ttcccgtgct
    30121 aaaataccag ggcttcgggc gcggtgtgaa cattacgctg ggcctgccct ttattcgcac
    30181 atcagtggac cacggcaccg cgcttgaact ggcgggacgt ggcgaagccg atgtcggcag
    30241 ttttattacg gcgcttaatc tcgccatcaa aatgattgtt aacacccaat gaataatcga
    30301 gtccaccagg gccacttagc ccgtaaacgc ttcgggcaaa actttctcaa cgatcagttc
    30361 gtgatcgaca gcattgtttc tgccattaac ccgcaaaagg gccaggcaat ggtcgaaatc
    30421 ggccccggtc tggcggcatt gaccgagccg gtcggcgaac gtctggacca gctgacggtc
    30481 atcgaacttg accgcgatct ggcggcacgt ctgcaaacgc atccattctt aggcccgaaa
    30541 ctgacgattt atcagcagga tgcgatgacc tttaactttg gcgaactggc cgagaaaatg
    30601 ggtcagccgc tgcgtgtttt cggcaacctg ccttataaca tctccacgcc gttgatgttc
    30661 catctgttta gctatactga tgccattgcc gacatgcact ttatgttgca aaaagaggtg
    30721 gtgaatcgtc tggttgcagg accgaacagc aaagcgtatg gtcgattaag cgtcatggcg
    30781 caatactatt gcaacgtgat cccggtactg gaagttccgc cgtcagcctt tacaccacca
    30841 cccaaagtgg attccgccgt cgtgcgcctg gttcctcatg caacgatgcc tcacccggtt
    30901 aaagatgttc gtgtgttgag ccgcatcacc accgaagcct ttaaccagcg tcgtaaaacc
    30961 attcgtaaca gcctcggcaa cctgtttagc gtcgaggtgt taacgggaat ggggatcgac
    31021 ccggcgatgc gagcggaaaa tatctctgtc gcgcaatatt gccagatggc gaactatctg
    31081 gcggagaacg cgcctttgca ggagagttaa cgatgatcaa ttcgccccga gtgtgtattc
    31141 aggttcaaag cgtctacatt gaggcacaat cttcacctga taatgaacgt tacgtttttg
    31201 cttataccgt aaccatacgc aatctggggc gagcgccagt gcagttgttg gggcgttact
    31261 ggctgatcac caatggcaat ggccgtgaaa ccgaagtcca gggcgaagga gtggttggcg
    31321 tccagccact tatcgcgcct ggcgaagagt accagtacac cagcggtgca atcattgaaa
    31381 ccccgctggg caccatgcag ggtcactacg aaatgatcga tgaaaatggc gtccctttca
    31441 gcatcgacat tcccgtattc cgactcgccg ttcccacact cattcattaa aagaatatgg
    31501 cgacatacct tattggcgac gttcatggtt gttacgatga actgatcgca ttgctgcata
    31561 aagtagaatt tacccctggg aaagataccc tctggctgac gggcgatctg gtcgcgcgcg
    31621 gcccgggttc gctggatgtt ctgcgctatg tgaaatcctt aggcgacagc gtacgtctgg
    31681 tgctgggcaa tcacgatctg catctgctgg cggtatttgc cgggatcagc cgcaataaac
    31741 cgaaagatcg cctgacaccg ctgctggaag cgccggatgc cgacgagctg cttaactggc
    31801 tgcgtcgcca gcctttgctg caaatcgacg aagagaaaaa gttggtgatg gcccacgcag
    31861 ggatcacgcc gcagtgggat ctacagaccg ccaaagagtg cgcgcgcgat gtagaagcgg
    31921 tgctatcgag tgactcctat cccttctttc tcgacgctat gtacggcgat atgccaaata
    31981 actggtcacc ggaattgcgg gggctgggaa gactgcgttt tatcaccaac gcctttaccc
    32041 gtatgcgttt ttgcttcccg aacggtcaac tggatatgta cagcaaagaa tcgccggaag
    32101 aggcccctgc cccactgaaa ccgtggtttg cgattcctgg ccctgtcgct gaagaataca
    32161 gcatcgcctt tggtcactgg gcatcgctgg agggcaaagg tacgccggaa ggtatatacg
    32221 cgctggatac cggctgctgc tggggtggta cattaacctg cctgcgctgg gaagataaac
    32281 agtattttgt ccagccgtcg aaccggcata aggatttggg cgaagcggcg gcgtcttaaa
    32341 cacagcctga tataggaagg ccggataaga cgcgaccggc gtcgcatccg gcactagccg
    32401 taaattctat acaaaattac cgccgctcca gaatctcaaa gcaatagctg tgagagttct
    32461 gcgcatcagc atcgtggaat tcgctgaata ccgattccca gtcatccggc tcgtaatccg
    32521 ggaaatgggt gtcgccttcc acttctgcgt cgatatgcgt cagatacagt ttctgcgctt
    32581 ttggcaggaa ctgctcataa acgcgaccgc cgccaatcac catgatttct ggtacgtcac
    32641 cacacgccgc gatggcttca tccaccgact tcacccacgt tacgcgatcg tccgtacccg
    32701 gttgactgct gaggataata tttttgcgtc ctggcaacgg acgaccgatt gattcccagg
    32761 tatggcggcc cataatcacg ggtttattta aggtgttgcg tttaaaccag gcgagatcgg
    32821 caggcaggtt ccacggcatg gcgttttcca tgccgataac gcgatctacc gctaacgccg
    32881 caatcagact gatcattgag atttcccgat aaaaaaaatt gtcgccacta tacgtaaagc
    32941 gtaaaccgtc gtcgactggt gcgaggatga tgttgaggaa aattttatat tctgctggcg
    33001 agtccacgct ctctccctgg actcgccgca ttacaatgaa acaaaaacaa acagttagct
    33061 gtaaagtgtg atttatgtca ctctttatta ggatgagggt ttcgtttccg gttcatccgc
    33121 catgttgccg gtatgtttac cttcttcggt tccctgccag ccatgtcgtt gaattaatga
    33181 cagatgttcg cggtcctcgg taatgatctc acttaacatc gcgctggtgc gtttatagac
    33241 cgccgcgcgg gctttggtgt cgttctcaac cattgccatc tcttccacca tctgaatatt
    33301 aaagcggcgg aacacatcgg cacgttctcg cgcttcatac ggccccagac ctaaactttc
    33361 cagtgccaga cgcccggttt tcagcgcacc ttcgaaggtt tcacgctccg gcttttcaac
    33421 gcctgcctga cgcaaacgaa tgtagtggtc gacatcgcgg gcgcgggcaa taatctgcaa
    33481 atgcgggaag tgttctttca ccatctctgt cagttgcagg ttggtttgcg gatcgtcgat
    33541 ggcgttaatc agcacttccg ctttcgccgc tcccgcagat tccagtaaat ccatccgcgt
    33601 ggcatcgcca taaaacactt tcataccaaa tttacgcaag gtttcgatat ggtccggatc
    33661 gtgatcgagc accaccattt tcaccccgct ggagagcagt aaacgtccgg tgatctgccc
    33721 aaaacgaccg aatccggcaa tgattacccg cggctgttct tcgtcgatct catcggcttc
    33781 acgcgcctct tcagtagaag attgctcaag gcgattgagg atcaccagca gaatcggcgt
    33841 tgccgccatc gacagcgcca ccgccagggt cagcgatttc gcccactccg gctccagcac
    33901 attcgccatc tgcgccgcgc caaataccac aaaggcaaac tcactgccct gccctaacaa
    33961 caccgcaaac caacgacgct gtttatttgg cacttgcaac ggtcgggcaa tcagccacag
    34021 catggcgatt ttgatgatga ggaaaccgag cagcaaaatg acaatgcgca atgggttttc
    34081 aagcagcgtg ccaaagtcta tcgacatgcc aacaccgatg aaaaacagcc ccaacagcaa
    34141 acctttaaag ggttcgatat cgctctccag cgcatgacgg tattccgagc ttgccagcag
    34201 tacgcccgcc agaaacgcgc ccatcgccat cgacaagccg acctcttcca gcagcaaacc
    34261 aaagccaaac acgaggaata acgccacggc actaaacact tcccgcaagc cagagcgggc
    34321 tacaaaacgc agcgccggac gcgtgacata gcgccccagc aataccacca gcaccagcgc
    34381 acccgccact tttaacgccg agagagcaaa tgcgcccatc gtcgtcgagg cactgctcgt
    34441 tgccagtagc ggaatcatcg ccaccagcgg gatcgccgcg atatcctgga acagcagcac
    34501 cgcaaaggca ctgcgaccca tttgcgtcac catcagattg cgttcattca tcgcctgcat
    34561 ggcaatcgcc gtagaggaga gcgccagcgt catgccgatc aattccgcga cctgccagcg
    34621 cagcccaagt aacatgcaga acagccccag caggccgccg caaatcacca tctgcaatgc
    34681 gccaccgccg aacactgccg cacgcagctt ccacagcctt tgtggatcga gttcgaggcc
    34741 gataataaac agcatcagca ccaccccaat ctcggcaaag tgcagaatag attcggcatc
    34801 ggtcaccagt cgcagccccc acgggccaat aatgcagccg gcgatcaggt agccaagtac
    34861 cgatcccaga ccaagacgta ccgcaatggg tacaatcagc gctgccgaac cgagataaat
    34921 cagcgcctga atcagcgtat ggctatccat gatgggcctc ctgccattcc agcagacgtt
    34981 gcttatagtg acgcgcctgc ccttcgaggg tttcgtcgtc acaaataaag gtgcagtgca
    35041 tggcaaacgg tggcagccag ttcagcccgc agtagattgc cgtcgcctgt agcggctgcg
    35101 acagcacatc aaagcccgga tgcgcaccaa tttcaaaatg gctttccccg ccgccggtcg
    35161 tcaccgccca cagcaaatgt ttgccatgca gcgccttgcc gccgtgaccg taagcccagc
    35221 cgtgcgacaa aactttatcg atccaaagtt taagaagcgg aggaatgctg taccactgca
    35281 tcggatgctg ccagacgatc agatcggcgc gagacagcgc ctcctgctcg gcggcaatat
    35341 cgatattgaa gtcaggatag agttgataaa gagagcgaat ttcgacgcct tccagcgtcc
    35401 ttgcctgttc aagcatccgt ttattcgcat gggaatgatg cggatacgga tgcgcataaa
    35461 ttataagaat cataggtagc ccgcgtgatt acgcctttgt tttcccacaa agtgtagtca
    35521 tatattcacg cggcttatag tcaataatat tgattcagaa aagagagaaa tctgaacaat
    35581 ttaccccatt ctctgcccgc ggatcgtcat attgctggtt tgcgccaatg acatccctcg
    35641 atgtctccgg ggcaaacgct acggaaatca acaagccaaa cagcgagata cccgccccca
    35701 ttagcatcgt gttactgata ccgtaattat tgataaagat cggcagtgcc caggtcgaaa
    35761 caatggtgcc aatacggctt aaggacataa tcacgcccac ggcagaggcg cggatatctg
    35821 tcgggaagag ttcattagga tagagccact gcaaattacc cggcccgcca gagaaaaagg
    35881 catacaccgc aaaggccatc actaccagcc agatccccat atccgggatc agccccaaaa
    35941 ccgccagcgc cagcgtcatc atggcaaagc tgccaatcaa caatggacgc cgtccggcag
    36001 tgtttaacca cagcatcggc ggaatacagc cgagcataaa gaacaggcta atcaccacgt
    36061 tccccagcgc cgcgtttttg ccaacaccca atcccaacaa accaacgatt tgcgggccaa
    36121 aggtgtaaat ggcgaacatt gggatcacct ggcaggtcca gatggcggca acaaacagaa
    36181 caaaaggaaa atggcggcga ttaaacagat cgcgaaaacg ggtttgctgc ggctgctctt
    36241 catcgaaagc caccggttcg ccaaacagtt ttatcatcat ctcttcgcac tcttttactc
    36301 gccctttgcg taataaccag cggggagatt caggcagttc gaatcgacca atcaaaatca
    36361 acaaacaggg gatcgccgcg ctacccagca tccagcgcca gccgccttcc acatcataaa
    36421 gccagtagcc gaccagatcg gcgcaggtcg cgccgacata ccacatggcg gcgataaagc
    36481 tgatggaaaa tgcccgctga cgggtactgg agaactcggt gatcattgag gtggcgatgg
    36541 gataatcggc accgatgaca atgccgataa gtacccgcat caccaacagt tcgacggggg
    36601 atgaaacaaa catcgtcgcc accgatatca cgccgatggc gatgatatca atgaggaaca
    36661 ttttgcgccg tccgacttta tcggaaatat agccaaacag cgatgtgcca acgaacagcc
    36721 cggcgagcgt tcccgcgccc aacaagccaa tccagtcagc gtccagtttt agcgccggag
    36781 tcagttgctc cagcgccacg ccaatcatta ccagtacata accatcca
//
LOCUS       DADLFA010000017        36522 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000017 DADLFA010000000
VERSION     DADLFA010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36522
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.017"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            24..896
                     /locus_tag="JQH65_000741"
     CDS             24..896
                     /locus_tag="JQH65_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001381404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY6138242.1"
                     /translation="MRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEEQIGYTLFT
                     RKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNTYPTIIFDQ
                     LISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELENLTISELPP
                     LRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGINPTIIYRPY
                     SFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSIRNHRYTAT
                     MLQHIAEDRDGTNH"
     gene            complement(893..2371)
                     /gene="scpC"
                     /locus_tag="JQH65_000742"
     CDS             complement(893..2371)
                     /gene="scpC"
                     /locus_tag="JQH65_000742"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HAY6138243.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETDKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(2395..3171)
                     /gene="scpB"
                     /locus_tag="JQH65_000743"
     CDS             complement(2395..3171)
                     /gene="scpB"
                     /locus_tag="JQH65_000743"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HAY6138244.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELGEAHT
                     MNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(3182..4177)
                     /gene="meaB"
                     /locus_tag="JQH65_000744"
     CDS             complement(3182..4177)
                     /gene="meaB"
                     /locus_tag="JQH65_000744"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HAY6138245.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNTLRLGVTGTPSAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKIRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIEFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(4170..6314)
                     /gene="scpA"
                     /locus_tag="JQH65_000745"
                     /pseudo
     CDS             complement(4170..6314)
                     /gene="scpA"
                     /locus_tag="JQH65_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
     gene            complement(6518..7411)
                     /locus_tag="JQH65_000746"
     CDS             complement(6518..7411)
                     /locus_tag="JQH65_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="HAY6138246.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYQFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            7794..8453
                     /gene="rpiA"
                     /locus_tag="JQH65_000747"
     CDS             7794..8453
                     /gene="rpiA"
                     /locus_tag="JQH65_000747"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAY6138247.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            8708..9940
                     /locus_tag="JQH65_000748"
     CDS             8708..9940
                     /locus_tag="JQH65_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAY6138248.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            10169..10228
                     /gene="ibsC"
                     /locus_tag="JQH65_000749"
     CDS             10169..10228
                     /gene="ibsC"
                     /locus_tag="JQH65_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HAY6138249.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            10561..10620
                     /locus_tag="JQH65_000750"
     CDS             10561..10620
                     /locus_tag="JQH65_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HAY6138250.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(10721..11323)
                     /locus_tag="JQH65_000751"
     CDS             complement(10721..11323)
                     /locus_tag="JQH65_000751"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAY6138251.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(11344..11527)
                     /gene="ssrS"
                     /locus_tag="JQH65_000752"
     ncRNA           complement(11344..11527)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="JQH65_000752"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(11569..11898)
                     /gene="zapA"
                     /locus_tag="JQH65_000753"
     CDS             complement(11569..11898)
                     /gene="zapA"
                     /locus_tag="JQH65_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAY6138252.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            12066..12644
                     /gene="ygfB"
                     /locus_tag="JQH65_000754"
     CDS             12066..12644
                     /gene="ygfB"
                     /locus_tag="JQH65_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HAY6138253.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            12670..13995
                     /gene="pepP"
                     /locus_tag="JQH65_000755"
     CDS             12670..13995
                     /gene="pepP"
                     /locus_tag="JQH65_000755"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HAY6138254.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIMNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     AEVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            13992..15170
                     /gene="ubiH"
                     /locus_tag="JQH65_000756"
                     /gene_synonym="visB"
     CDS             13992..15170
                     /gene="ubiH"
                     /locus_tag="JQH65_000756"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HAY6138255.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            15193..16395
                     /gene="ubiI"
                     /locus_tag="JQH65_000757"
                     /gene_synonym="visC"
     CDS             15193..16395
                     /gene="ubiI"
                     /locus_tag="JQH65_000757"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HAY6138256.1"
                     /translation="MQCVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADISLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(16469..16552)
                     /locus_tag="JQH65_000758"
     CDS             complement(16469..16552)
                     /locus_tag="JQH65_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6138257.1"
                     /translation="MSKNTKSKNNDIRKYNAKTEVKLVYFK"
     gene            16842..17936
                     /gene="gcvT"
                     /locus_tag="JQH65_000759"
     CDS             16842..17936
                     /gene="gcvT"
                     /locus_tag="JQH65_000759"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HAY6138258.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            17960..18349
                     /gene="gcvH"
                     /locus_tag="JQH65_000760"
     CDS             17960..18349
                     /gene="gcvH"
                     /locus_tag="JQH65_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HAY6138259.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            18468..21341
                     /gene="gcvP"
                     /locus_tag="JQH65_000761"
     CDS             18468..21341
                     /gene="gcvP"
                     /locus_tag="JQH65_000761"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HAY6138260.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETSISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSHEVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            21607..22350
                     /locus_tag="JQH65_000762"
                     /pseudo
     CDS             21607..22350
                     /locus_tag="JQH65_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(22407..23840)
                     /locus_tag="JQH65_000763"
     CDS             complement(22407..23840)
                     /locus_tag="JQH65_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAY6138261.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEIPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMINAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            23885..24196
                     /locus_tag="JQH65_000764"
     CDS             23885..24196
                     /locus_tag="JQH65_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAY6138262.1"
                     /translation="MQPNDITFSQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            24360..25019
                     /locus_tag="JQH65_000765"
     CDS             24360..25019
                     /locus_tag="JQH65_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAY6138263.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSMCHFLAIYLYIGQ
                     A"
     gene            complement(25215..26195)
                     /gene="ygfZ"
                     /locus_tag="JQH65_000766"
     CDS             complement(25215..26195)
                     /gene="ygfZ"
                     /locus_tag="JQH65_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HAY6138264.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTENQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAADDERVLLGVAGFQARAALANLFSELPSREKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            26438..26704
                     /gene="sdhE"
                     /locus_tag="JQH65_000767"
     CDS             26438..26704
                     /gene="sdhE"
                     /locus_tag="JQH65_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HAY6138265.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            26685..27092
                     /locus_tag="JQH65_000768"
     CDS             26685..27092
                     /locus_tag="JQH65_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin CptA"
                     /protein_id="HAY6138266.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(27132..27653)
                     /gene="fldB"
                     /locus_tag="JQH65_000769"
     CDS             complement(27132..27653)
                     /gene="fldB"
                     /locus_tag="JQH65_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HAY6138267.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            27765..28661
                     /gene="xerD"
                     /locus_tag="JQH65_000770"
     CDS             27765..28661
                     /gene="xerD"
                     /locus_tag="JQH65_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAY6138268.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            28686..29396
                     /gene="dsbC"
                     /locus_tag="JQH65_000771"
     CDS             28686..29396
                     /gene="dsbC"
                     /locus_tag="JQH65_000771"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000715205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HAY6138269.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGAAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLESQAEQQMKAIW
                     CAKDKNKAFDDVMAGKAATPASCDIDIADHYALGVQLGVSGTPAIVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGN"
     gene            29402..31135
                     /gene="recJ"
                     /locus_tag="JQH65_000772"
     CDS             29402..31135
                     /gene="recJ"
                     /locus_tag="JQH65_000772"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAY6138270.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQIEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            31226..32324
                     /gene="prfB"
                     /locus_tag="JQH65_000773"
     CDS             join(31226..31300,31302..32324)
                     /gene="prfB"
                     /locus_tag="JQH65_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAY6138271.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            32334..33851
                     /gene="lysS"
                     /locus_tag="JQH65_000774"
     CDS             32334..33851
                     /gene="lysS"
                     /locus_tag="JQH65_000774"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAY6138272.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(33894..34442)
                     /gene="idi"
                     /locus_tag="JQH65_000775"
     CDS             complement(33894..34442)
                     /gene="idi"
                     /locus_tag="JQH65_000775"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAY6138273.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTNSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            34497..34568
                     /locus_tag="JQH65_000776"
     CDS             34497..34568
                     /locus_tag="JQH65_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6138274.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(34565..34690)
                     /locus_tag="JQH65_000777"
     CDS             complement(34565..34690)
                     /locus_tag="JQH65_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6138275.1"
                     /translation="MNLLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(34692..36140)
                     /locus_tag="JQH65_000778"
     CDS             complement(34692..36140)
                     /locus_tag="JQH65_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAY6138276.1"
                     /translation="MSAIDSQLLSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSVGL
                     SIIQVGIDWAAGGKGNPQYGNPVYSGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDALWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
BASE COUNT         8792 a         9197 c         9766 g         8767 t
ORIGIN      
        1 tggacatttt tatttcgaaa aagatgcgca atttcatttt attagcgcaa accaataata
       61 ttgcccgggc ggcagaaaaa atccatatga cggcttcgcc atttggtaaa agcattgccg
      121 cgctggaaga acaaattggc tatacgctat ttacccgcaa agataataat atcagcctca
      181 ataaagccgg tcaggaactg tatcaaaaac tgttcccggt ttatcaacga ctttctgcga
      241 ttgataatga aatccataac tcagggcgtc gttcacgaga aattgtgatt ggcatagata
      301 atacttatcc gacaattatt ttcgatcagt tgattagtct cggagataag tatgaaggcg
      361 tgaccgccca gccagttgag ttcagcgaaa acggcgttat cgataatctt tttgaccgtc
      421 agttagattt tattatctcc ccacagcatg tatctgcacg agttcaggaa ctggaaaacc
      481 tgactatcag tgaattacca ccgctgcgat tgggctttct cgtttctcgc cgttatgaag
      541 agcgtcaaga gcaagagctg cttcaggagc tgccctggct acaaatgcgc tttcaaaacc
      601 gggctaactt cgaagcaatg attgatgcga atatgcgacc ttgtgggatc aatcccacca
      661 ttatttatcg tccctacagt tttatggcca aaatcagcgc cgtggagcgc ggacattttc
      721 tgacggtgat cccgcatttt gcctggcgac tggtgaatcc ggcaacgctg aaatattttg
      781 atgcgccgca caggccgatg tatatgcagg aatatcttta ttcaatcaga aatcatcgtt
      841 ataccgccac gatgcttcag catattgctg aagatcgtga cgggacaaac cattaaccca
      901 gcatcgagcc ggttgcaatt aaattacggt gtaagtcgaa gacgtggcta agatcgtggt
      961 gaatatgtcc gccgggcgca ttttccagat agcgatgcag ataatcccga tacataggat
     1021 gtgcacaatt atcaatgata gtgcgggcgc gttgaagcgg ggaaagaccg cgcagatcgg
     1081 cgatcccttg ttcggtgatg atcactttga cgctgtgttc gctgtgatca acatggctgc
     1141 acattggcac gacggttgag atcttgcctt ctttagcaat cgacggagcc ataaagatcg
     1201 acagatacgc gttgcgttca aaatcaccgc tgccgccgat gccgttcatc agatcgaccc
     1261 cggctacgtg cgttgaattt gcatgcccgt aaatatcaaa ctccaggccg acgttcagag
     1321 cgatgacgcc cagacgacgg atgatttccg ggttattgga aatctcctgc ggacgcaaca
     1381 caatgcggct ggcaaagtaa tccatattgt cgtaaatctt gcgcagggaa tcggccgaga
     1441 ttgtcaggct ggaggcgctg gccccgctga ttttgtcggt ttccagtaaa tgcaccactg
     1501 attcctgtag cacttccgaa tacatcatga acggaggaat ttccgggttt tcccccagac
     1561 gcgccattac cgcattattg atattgccca cgccactttg cagcggcaga aattccggcg
     1621 gaatacgccc atgcgccatt tcctgcaata agaacgtgac cacgttatcg gcaatctgct
     1681 ggcacatggg attttgctta tccagcatat taccggcgtc gggcaagttg gtttccacga
     1741 cggcgacaat ctttttcgga tcgatttgca catagcgggt accgacgcga tccattgcat
     1801 ggaagatcga cacgctattg cgccgcggtg gcgcgccagg aatcacaata tctgccagtt
     1861 ctgcaacgcg cggatcgtga tagtgattaa gttcaatgat cactttcttc gctcgcagca
     1921 gccaggtcgg cgcattaccg atcccgctgg ttaaccagac tcgaccatcc ggtgccagtg
     1981 ccgatgcttc aatgacggca acatcaatgt cgccgaagaa accgtaattg accatttgcg
     2041 ccacttcgct caaatgcagg tcaacgaaac tcaccgcgcc ctgattgatc tttttacgta
     2101 aaccggacga tgtttgatat ggcgcacgcc aggaaacagc atcggcgtca gaaagtacat
     2161 cgtcagcggc ggcgctgatt gacgcccccg tcagcaggcg aatttgatac ggctttttgg
     2221 cctcatgctg ttcgttagct ctgcgggcaa tcgcggtggg tagggctttc ggcgaacccg
     2281 ccggggtaaa gccgctaaat gccaccatgt cgttatgctg gataatttct gccgcttcat
     2341 cggcggtcat ccttgtccac tgagtttcca tttttactcc ttcgttcgca gggattaatg
     2401 accaacgaaa ttaggtttac gtttttcgag gaaagcgttc attccttcct ggtaatcttc
     2461 gctgtcataa accgcgcgtc gcatcccctg aatacgttca aattcatcgg agttcatggt
     2521 gtgcgcttcg cccagctctt ctttgataac agcaatggct aacggcgctt tctcagagat
     2581 gtggtgcgcc atttgtaagg tgaaatcttc cagttcttcc acttccacaa catggttgag
     2641 gatgccgaca gccagcgcgc gctgggcggt gattggcgaa gcggtaaaaa tcagctcttt
     2701 gacaatgtgg aagcccgcgt cgcgggtcag gttgtgaatg ccgaccaggt tatacgggac
     2761 gccgaggttt acaggcgtca ttgagaaggt tgaggtactg gcggcgatga tcagatcgga
     2821 actcatgatc atttcaaatg cgccacccca aacactacct tccaccatcg aaatgatcgg
     2881 tttcgggaat ttttggatca tgcgggtgat ttgacgcaat ggatcatcat aggagagcgg
     2941 atcgcgaccg ccagacggca gttcgtgaat atcgtgacct gcggagaaga ctttggatcc
     3001 actcggcgcg cgcaaaatga tacagcgaat ttccggccgg ttgagatcgc ttaacgcctg
     3061 cataagatca tcaataaaga ctttacttaa ggcattaagt tttcggccat agttaaactc
     3121 aatgaccgcc actttgttga tagtggcaac gttcacatac tgataagaca taaaaattcc
     3181 tttaatcaaa atattgcgtc tggataaatt cactgagctg ccgcaggccg gtgcgcggtg
     3241 agagcgtatt gtttttgacc gctaaaagcg tctggcggta atagcgatcg aaatcttcat
     3301 tcgcgaacag gtgattcagt acttcttctt cggtctgctt acgcagccat tccaccgatt
     3361 gttgttgccg cacttgttgt aaacgaccac tggcagttag cgcggttttg aactcgatga
     3421 tggcgtgcca gatctcatcg attccacgtt tttccagtgc gctacaagtc agaacccgtg
     3481 gctgccattc gtcgtattta cgtcgcagaa tatgcagggc actctcgtac atatgccggg
     3541 caatggcgac attggtatgg ttatcgccat cgtctttgtt gataacgatc agatcagcca
     3601 cttccatcag ccctttttta atgccctgca gatcatcgcc gccaccggca atttgcaacg
     3661 agataaaaca gtccaccatg cgggcgactt ctgtttccga ctgcccgacg ccaaccgttt
     3721 cgacaatcac tacgtcataa cccgccgctt cgcataacag cattaattcc cgcgctcgct
     3781 gactggcacc gcccagatga ccggaggatg gtaccgggcg aataaacgcc gcttcggcac
     3841 gcgccaggtc attcatgcgg attttatccc cgagaatgct accgccagtg accgggctgc
     3901 tgggatcgac cgcaataacc gcgaccttta atccctctcg aatcaacaac atgccaaagg
     3961 cctcaagaaa ggtacttttc cccgcgctgg gggtgccggt aacgcccagt cgcagggtgt
     4021 taccgcagta cggcataatg gcatcaagca actgcgtact tagtgcctga tgacgcgggt
     4081 gacggctttc caccagcgtc atggcctggg cgagtgtggc acgctcaccc tgacgtaagc
     4141 ggcgaatact ttctgccagc gtggcttcat taatcatgat gctggcttat cagattcagt
     4201 acgtcgcgca cactgtcgag cataggtgta cctggaccat aaatcgccgc cacgccgcgc
     4261 tcttgcagga aggcgtaatc ctgcggtgga atgacgccac cggcgactac gcagatatct
     4321 tcgcgtcccc attttttcag cgcctcgacc agttccggga tcagcgtttt atgaccggca
     4381 gccagtgagg atgcgcccac tacgtgaacg tcattttcta ctgccaggcg ggcgatctct
     4441 tcaggtgtag agaacatcgg gcttaaatct acgtcgaaac cgagatcgga ataggcgctg
     4501 gcgatcactt tcgagccgcg atcgtgccca tcctggccca ttttggcgat cagaatgcgc
     4561 aggcgacgac cattgtcggc aaggaactgc tccgtttgcg caacaatggc atcgaactcg
     4621 gaggccgatt tctcagattg atgatagctt tgcgcaatca cgccggtaac acactggctt
     4681 ggcaccagat aacggtcgaa agcggcttcc agcgcatcgg aaatttcacc cagggtggcg
     4741 cgaacgcgag cggcattaac agcggcagcc agcaggtttt cgttatgctg tgcggcgtga
     4801 gtcagggcgt tcaacgcggc ggttacggcg gcatcatcac gggtggcgcg aatgcgttcc
     4861 agcgaagcaa tttgctcgtt acgcaccatc acgttgtcga tctcaagtac atcggtttcg
     4921 tcttcgtgat ccagtttgta cttgttgaca ccaacgatga cacgtttgcc ctggtcgata
     4981 agcgactgtt cgcgcgctga ggcctattcg atcattcgtt ttggcagacc tgcttcgatc
     5041 gctttcgcca taccaccggc ttcgtcgatc tgttggataa tagctctggc ttgtttgacg
     5101 atttgatcgg tcagcgactc aatgtaatag gatccggcca gtggatcgac ggtgcggcag
     5161 agttctgatt cttcctggat gatgatctgg gtgttgcggg caatgcgtgc tgagaaatcg
     5221 gtaggcaaac caagcgcttc gtcaaaggcg ttggtatgca gtgactgagt accgcccagc
     5281 gtcgcagcca gtgcttcaat ggtggtgcgg ataacgttgt tatacggatc ctgttcagtc
     5341 aggctccagc ctgaggtctg gcagtgggta cgcagcgcca gtgatttcgg gtcctgtgcg
     5401 ccaaatccac tgaccgcttc gctccataaa taacgtgccg cacgcaacat ggcgacgttc
     5461 ataaacagat ccatgccgat gccgaagaag aacgacaggc gaggagcgaa gtcatcaatt
     5521 ttcagtccgg cagagattgc tgctttgatg tactcaatcc catcagcgag cgtaaatgct
     5581 acctgctgca cgcagttggc acccgcttca cccatgtggt aaccgctgat actgatggta
     5641 ttaaatcgcg gcatgttgcc ggaacaccag gcgatgatgt cggcgataat gcgcattgac
     5701 ggttttggcg ggtaaatata ggtgttgcgg cagaggtact ctttgaggat atcgttctga
     5761 atggtgccgg tcagtttatc aggtgtaaca ccttgctctt ccgcggcgac gatataaaac
     5821 gccagtactg gtagcactgc gccattcatg gtcatcgaaa ccgacatttt atccagcggg
     5881 atctggtcga acaggacttt catatcttcc acggtgtcga tagcgacgcc cgctttgccg
     5941 acgtcgcccg ccacgcgcgg gttatcggag tcgtagcctc ggtgggtggc aaggtcaaac
     6001 gcaacggaaa gacctttttg cccggcggcc aggttacggc gataaaaagc gttggactct
     6061 tttgctgttg aaaaaccagc atactgacgg atggtccacg gttgggcggt atacatagtg
     6121 gcacgcgggc cacgaacgta gggcggcaaa ccaggaaggg tacctgtcac ctccagatta
     6181 tcgagatcgg cttcggtata cagcggcttg atggtgatcc cttccgcggt ttgctgaacc
     6241 agcgagtcga cagttttctc ccgacggctc aattccttgt tggcaagctg ttgccactcc
     6301 tgcacgttag acattatttg ctccgtaaaa cgcaatcaaa atgagggtat ttgcgcctac
     6361 ggtgagcttt ttacgtgggt ttgtcgccag caaaaaaaat gcattcagac gtcgtttttt
     6421 gccgcctgag atttgtctat tttgtgatgt gattcaagga atacaaaaag aagtgaggac
     6481 aacgcctgat atgtgctatc aggcattatt tgatggatta atcctgacga aggactttgt
     6541 gaccataatc gagcaacgca tcagtgactt tacgcatcat gcggctttca ggagcaaagc
     6601 ggtgccagta gagcatccgg cgttgaaata gccctggcgt taagtcaatc agttcaccgc
     6661 tggctagttc tttctcgatt tgcaggtgcg ggatcataca gcaggtggtg ccctggcgag
     6721 caagttgcac gaacgcttct gaagaattaa cgatatggca gggcacgctg cctggaggca
     6781 gatcgaagtt ttgctgcaaa aaggcctggt gcatatcgtc aagatggtca aaggcgacca
     6841 caggtgcttt caataatgcc gaacgcgtta cgccgttagg gaaatatttt tcggcaaacg
     6901 gttttgagct gacgaactga tagtcaagcg caccgagctt atcgacaagg caactcggca
     6961 gcgcctgatg ttgaatactc accgcgccga ccacttcgcc gcggcgcaga cgttcctgag
     7021 tacgggtttc atcttctacc tgcaagttga ggcggatagg cgaatcagcc aatacaggag
     7081 ccagtgccgg aagcaaccac gtcgccagac tgtcggcgtt gaccgccagt gaaagcagca
     7141 gcggagtcga accggtttgt tcatcgccca gccactcttc ttccagcaac tccacctggc
     7201 gcagcagtgc cagcagtttt tgcccttgtt ccgtcgggcg cggcggtacg gtacgcacca
     7261 acagcggctg cccgaacata ttttccagtt gcttaatgcg ctgtgagacg gctgattgtg
     7321 taatgcacag cttttgtgcc gcgcgctcaa atcctcgttc acgtatcacc gcatccagtg
     7381 cctgtaatgt tctgtagtcc gggcgtttca ttgctgttgt ctgctcctgg taaatgcttt
     7441 ttcctgcact atgacacaat tttgtgtcag gttgcatata ccgcagccgt cggcgggtaa
     7501 catttgtatc aatggttaaa cgcgcagctt aaaagatcag ggatttgcgc tattgcattt
     7561 cgcttagcga cttcgcgccc tggttatgtt gaatggcgtg gcgttattgc ctctgttcgc
     7621 accgtggagc ggcataccca gaccgttgta tgctttattt gcggggcgaa aggggatgcc
     7681 cgccattgcg cgatatttta tggatgagtt aaccacccgg cttgccaacg gggtctgaat
     7741 cgcttttttt gtatataatg cgtgtgaaat ttcataccac aggcgaaacg atcatgacgc
     7801 aggatgaatt gaaaaaagca gtaggatggg cggcacttca gtatgttcag cccggcacca
     7861 ttgttggtgt aggtacaggt tccaccgccg cacactttat tgacgcgctc ggcacgatga
     7921 aaggccagat tgaaggggcc gtttccagtt cagatgcttc cactgaaaaa ctgaaaagcc
     7981 tcggcattca cgtttttgat ctcaacgaag tcgacagcct tggcatctac gttgatggcg
     8041 cagatgaaat caacggccac atgcaaatga tcaaaggcgg cggcgcggcg ctgacccgtg
     8101 aaaaaatcat tgcttcggtt gcggaaaaat ttatctgtat tgccgacgcc tccaagcagg
     8161 ttgatattct gggtaaattc ccgctgccag tagaagttat cccgatggca cgtagtgcag
     8221 tggcgcgcca gctggtgaaa ctgggcggtc gtccggaata ccgtcagggc gtggtgaccg
     8281 ataatggcaa cgtgatcctc gacgtccacg gcatggaaat ccttgacccg atagcgatgg
     8341 aaaacgccat aaatgcgatt cctggcgtgg tgactgttgg cttgtttgct aaccgtggcg
     8401 cggacgttgc gctgattggc acacctgacg gtgtcaaaac cattgtgaaa tgatctgacg
     8461 ggggaacctc ccccgttaaa aaaattctct tcattaaatt tggtgacatg tgtcacgttt
     8521 ttacctggct attgtcgatt gctctaaata aatcctctaa accagcatat tcaaccaaga
     8581 aatacctttg cgtgatattt cctcaacatc gcgacgcaaa cgttcatatt gccgcaatat
     8641 tattttttga tatgttgaaa ggcggatgca aatccgcaca caacatttca aagacaggat
     8701 tgggtaaatg gcaaaggtat cgctggagaa agacaagatt aagtttctgc tggtagaagg
     8761 cgtgcaccaa aaggcgctgg aaagccttcg tgcagctggt tacaccaaca tcgaatttca
     8821 caaaggcgcg ctggatgatg aacaattaaa agaatccatc cgcgatgccc acttcatcgg
     8881 cctgcgatcc cgtacccatc tgactgaaga cgtgatcaac gccgcagaaa agctggtcgc
     8941 tattggctgt ttctgtatag gaacaaacca ggttgatctg gatgcggcgg caaagcgcgg
     9001 gatcccggta tttaacgcac cgttctcaaa tacgcgctct gttgcggagc tggtgattgg
     9061 cgaactgctg ctgctattgc gcggcgtgcc ggaagccaat gctaaagcgc accgtggcgt
     9121 gtggaacaaa ctggcggcgg gttcttttga agcgcgcggc aaaaagctgg gtatcatcgg
     9181 ctacggtcat attggtacgc aattgggcat tctggctgaa tcgctgggaa tgtatgttta
     9241 cttttatgat attgaaaata aactgccgct gggcaacgcc actcaggtac agcatctttc
     9301 tgacctgctg aatatgagcg atgtggtgag tctgcatgta ccagagaatc cgtccaccaa
     9361 aaatatgatg ggcgcgaaag aaatttcact aatgaagccc ggctcgctgc tgattaatgc
     9421 ttcgcgcggt actgtggtgg atattccggc gctgtgtgat gcgctggcga gcaaacatct
     9481 ggcgggggcg gcaatcgacg tattcccgac ggaaccggcg accaatagcg atccatttac
     9541 ctctccactg tgtgaattcg acaacgtact tctgacgcca cacattggcg gttcgactca
     9601 ggaagcgcag gagaatatcg gcctggaagt tgcgggtaaa ttgatcaagt attctgacaa
     9661 tggctcaacg ctctctgcgg tgaacttccc ggaagtctcg ctgccactgc atggtggtcg
     9721 tcgtctgatg cacatccacg aaaaccgtcc gggcgtgcta actgcgctga acaaaatctt
     9781 cgccgagcag ggcgtcaaca tcgccgcgca atatctacaa acttccgccc agatgggtta
     9841 tgtagttatt gatattgaag ctgacgaaga cgttgccgaa aaagcgctgc aggcaatgaa
     9901 agctattccg ggtaccattc gcgcccgtct gctgtactaa ttccccttct ctgaaaatta
     9961 acgggcaggt cactgacttg cccgtttttt tatcccttct ccacaccgtt gccgcgttgc
    10021 gcattctact ttgcgagtct gctccagaaa ataaatcaaa gtcagatcaa tgcgttgtgt
    10081 ttatttaatg gggatttacg atggcgggac agtatggggc tgtaacggta aagccctcac
    10141 cgaagcgagg gcttaaagga gaagggttat gatgcgactt gtcatcatac tgattgtact
    10201 gttactgata agtttcggag cttattaaca gtcagtctct ggggaggagt catcctccct
    10261 tacccttact cactaaatta ggtcaaagaa tcaacgatgt caatcagggc gatgcggggt
    10321 gcatcgccct gaaccgaact ttttgatagc cacttcatcg cccacgttta ctgccgtaat
    10381 agatcctttt tcccaaaaat tatctctgtt ttgcatgggg tttttcgaac acgctcgaag
    10441 aaaagattta aaggtagatc aatgcgttgt ggttatttaa agagagctta cgatagctgg
    10501 gcagcatggg gctgtaacgg taaagccctc accgaagcga gggcttgaag gagaagggtt
    10561 atgatgcgac ttgccatcat actgattgta ctgttactca taagtttccc agcttattaa
    10621 cagtcagtca caggggagga gcaatcctcc cttaccctta ctcactaaat taggttaaag
    10681 aatcaacgat gtcaatcagg gcgatgcagg tgtatcgccc ttaccactcc cagactttcg
    10741 acggtgtaac caccgcagga agagggatat cccactcttc aacggggagt ttttccacca
    10801 actgacaatc atgcgcataa cccaccggtt gcgttttata gtgctgccag ttttgtaagg
    10861 tccgatcata aaaaccaccg cccattccca ggcgctgacc gtactcatca aaggcgacca
    10921 gcggtgtgat cagcacgtct aatcgggaaa ggggtagcac gtcacgcaca tccagttttg
    10981 gctcatggat tttcaacctg ttcatcacca gttcgctttg cggatggtaa ttcaggaaca
    11041 gcaaattacc agcactaaag ggatgcaaaa ctggaagata tacgcgctta ccggcgcgcc
    11101 agagttgttc tattagcggc tgggtgtcga gttcaccatc aaaagagagg aatacagcga
    11161 ccgtatgcgc catcaccacc gggggataag tcatcatccg ggtagcggct tgttgaccca
    11221 tttcctgctg ttgttccggc gttaacgcac gacgacgttg ccgaatcatt ttgcggattt
    11281 cttgtcggga taatgtcagt gggagttctg gtagttgcgt catggctggt gccagataag
    11341 aagggaatct ccgagatgcc gccgcaggct gtaacccttg aacccttggt tcaaggtgaa
    11401 tgtgtcgtcg cagttttaag gcttctcgga cggaccgagc atgctcacca accgcggagc
    11461 gccacattct tgtggtatga aatatcggct caggggactg gcccgcttgc gaacatctca
    11521 gagaaatttt gtcttcacgg ttactctacc acagtaaacc gaaaagtgtc attcaaagtt
    11581 ttggttagtt ttttcggtga tgcgaccttg ttcaagtaac gcttgttcta tggtctgctg
    11641 cagcatccga atacgctgtt ccatacttgc cgcgtagtca cgagtcttcg ctttttcttg
    11701 cgctaactca tagctgatat tcaatgcggc aatgaagacc aactgttcag tatttgtgac
    11761 tctagtgcgt tctttcagat cttgcaaccg ttggttcaga tcgtccgctg cctgattcaa
    11821 cgcatccctt tggtcaggcg ggcagttcac acgcagtgaa cggccaaaaa tttggatatc
    11881 gacgggttgt gcagacatgc caccttcctg ctgattgact gcgctgcctt cgtctttaga
    11941 cccatggtct gcgaaggggc gacactatag ctaccctgat gagaagagac aagccctttt
    12001 ctggtccacc agggcccaaa gtggtagcat atcatgaata ttcctccctt tgacgacgaa
    12061 tgcttatgtc tatacagaac gaaatgcctg gttacaacga aatgaaccag tatctgaacc
    12121 aacaagggac gggtctgacc cctgccgaga tgcatggttt aatcagtggg atgatatgtg
    12181 gcggtaacga tgacagctca tggctgccgc tacttcacga cctgacgaac gaaggcatgg
    12241 ctttcggtca tgagctggca caggcactgc gtaaaatgca ctctgccacc agcgatgccc
    12301 tgcaggatga cggcttcctt tttcagcttt atctgcctga tggcgatgat gtcagcgttt
    12361 tcgatcgggc tgatgcattg gcaggttggg tcaatcactt cctgcttggt cttggcgtta
    12421 cgcaaccgaa gctggataaa gtgaccggcg aaaccggtga agctatcgac gatctgcgta
    12481 acattgcgca actgggttac gacgaagacg aagatcagga agagcttgaa atgtcgcttg
    12541 aagagatcat cgaatacgtt cgtgttgccg cgctgttatg ccacgacacc tttactcatc
    12601 cgcaaccgac cgcgccagaa gtacaaaaac cgactctaca ctaaaaacaa aaaacgtaag
    12661 gagagtgtta tgagtgagat atcccggcaa gagtttcagc gtcgccgtca ggccctggtg
    12721 gagcaaatgc aacccggcag cgccgcgctg atttttgctg caccagaagt aacacgtagc
    12781 gccgacagcg aataccccta tcgtcagaac agtgacttct ggtacttcac cggctttaac
    12841 gaaccggaag cggtgctggt gctgattaaa agcgatgaca ctcataacca cagcgttctg
    12901 tttaaccgcg ttcgcgacct gacggcggag atctggtttg gccgtcgctt aggccaggat
    12961 gccgcgccag agaaactggg cgttgaccgc gcactggcat tcagcgaaat caatcagcaa
    13021 ctttatcaac tacttaacgg cctggatgtg gtttaccatg cccagggcga atatgcatat
    13081 gctgatgaaa tcatgaacag tgcgctggaa aaactgcgta aaggttcgcg gcaaaatctc
    13141 accgcaccgg caacgatgat cgactggcgt cctgttgttc atgaaatgcg cctgttcaaa
    13201 tcgccagaag agattgccgt actccgccgc gcgggagaaa tcaccgccat ggcacataca
    13261 cgggcgatgg aaaaatgccg tccgggaatg ttcgagtacc atctggaagg cgaaattcac
    13321 cacgaattta accgccacgg tgcgcgctat ccgtcctata acaccattgt cggcagcggt
    13381 gaaaacggct gcattctgca ctacaccgaa aacgagagtg aactgcgcga cggtgacctg
    13441 gtgttgattg acgcgggttg tgaatacaaa ggttacgcgg gcgatattac ccgcaccttc
    13501 ccggtcaacg gcaaattcac ccaggcccag cgtgaaatct acgacattgt gctggagtct
    13561 ctcgaaacca gcctgcgcct gtatcgtccg ggaacttcca ttctggaagt cactggtgaa
    13621 gtggtgcgca tcatggttag cggcctggta aaactcggca ttctgaaagg tgatgttgat
    13681 gaactgatcg ctcagaatgc ccatcgtcct ttctttatgc atggccttag ccactggtta
    13741 gggctagatg tccatgacgt tggcgtttat ggtcaggatc gctcgcgcat tctggaaccg
    13801 ggcatggtac tgaccgtaga gccagggctg tatattgcgc cggatgcaga agtgccagaa
    13861 caatatcgcg gtatcggcat tcgtattgaa gacgacattg tgattaccga aaccggtaac
    13921 gaaaacctca ccgccagcgt ggtgaaaaag ccggaagaaa tcgaagcgtt gatggctgct
    13981 gcgagaaagc aatgagcgta atcatcgtcg gtggcggcat ggcgggcgcg acgctggcgc
    14041 tggctatttc ccggttaagt cacggggcgc tgccggtaca tttgattgaa gcgactgcgc
    14101 cagagtcaca tgctcatccg ggctttgatg gacgagcgat agcgctggcg gcgggtacct
    14161 gtcagcaact ggcgcgcatc ggcgtctggc aatctctggc ggattgcgca actgccatca
    14221 ccaccgtgca tgtcagcgat cgtggtcacg ctggatttgt caccctcgcc gcagaagatt
    14281 accaactggc ggcgctggga caggttgtcg aattgcacaa tgtcgggcaa cggctgtttg
    14341 cattgctgcg taaagcacct ggcgtaacgc tgcattgccc tgatcgcgtg gctaacgttg
    14401 cccgtactca gagtcacgtt gaagtgacgc tggagagtgg cgagacgctg acgggccgcg
    14461 tgctggtagc agctgatggc acccattcag cgttagccac cgcgtgcggc gttgactggc
    14521 agcaggagcc ttacgaacaa ctggccgtga ttgccaacgt tgcgacttcc gttgcgcacg
    14581 aaggccgcgc ttttgaacgc ttcacgcaac atggcccgct ggcgatgttg ccgatgtctg
    14641 acggacgctg ttcgctggtc tggtgtcatc cactggaacg gcgcgaagag gtgctgtcgt
    14701 ggagcgacga gaagttttgc cgtgaactcc agtcggcctt tggctggcga cttggaaaaa
    14761 ttacccacgc cggtaaacgc agtgcttatc cgctggcgtt aacccgcgcc gccagagcta
    14821 ttacccatcg taccgtgctg gtgggcaatg cggcgcaaac tctgcacccg attgccgggc
    14881 aagggtttaa cctcggtatg cgagatgtca tgagccttgc ggaaaccctg actcaggcgc
    14941 atgagcgcgg agaagacatc ggtgattacg gcatattgtg ccgctatcag cagcgtcgac
    15001 agagcgatcg cgaagcaacc atcggcgtca cggacagcct ggtacatctt tttgccaacc
    15061 gttgggcacc gctggttgtc gggcgcaaca tcggactgat gacgatggaa ttattcaccc
    15121 cggcacgcga tgtgctggcg cagcgcaccc tcggttgggt ggcgcgttga aggcggtttt
    15181 aaggaataaa caatgcaatg tgttgatgta gccattgttg gtggcggcat ggtggggctg
    15241 gcggttgcct gcggcttaca ggggagcggc ttacgcgttg ccgtactgga gcagcgcgta
    15301 ccggaacctc tggcggcgga tgcaccacca caactgcgcg tttcggctat caatgccgcc
    15361 agcgaaaaat tactcacccg tcttggcgtc tggcaggaca ttctctctcg tagggccagt
    15421 tgttatcacg gtatggaagt gtgggacaaa gacagttttg gtcacatttc gtttgacgat
    15481 caaagcatgg gctatagcca tcttgggcat atcgttgaaa actcagtgat tcactacgcg
    15541 ctgtggaaca aagcgcagca gtcgtcagat atcactctgt tggcccccgc agaattacag
    15601 caggtcgcct ggggagaaaa tgaaactttc ctgacgctga aagatggcag catgttaacg
    15661 gcgcgtctgg tgattggcgc ggacggtgct aattcctggt tgcgcaacaa agcagatatt
    15721 tcgctgactt tctgggatta tcagcatcac gcgctggtag cgaccattcg cacggaagaa
    15781 ccgcatgatg cggtggcgcg gcaggttttc catggcgaag gcattctggc ctttttaccg
    15841 cttagcgatc cgcatctttg ctcaattgtc tggtcactgt cgccagagga agcgcagcgg
    15901 atgcagcagg caagtgaaga tgaatttaat cgcgcgttaa atatcgcttt tgataatcgc
    15961 ctgggcttat gcaaggttga gagcgcgcgt caggtgttcc cactgacggg gcgttatgcg
    16021 cgccagtttg ccgcgcaccg tctggtgcta gtgggcgacg ccgcgcatac cattcacccg
    16081 ctggcggggc agggagtaaa tctcggcttt atggatgctg cagagctgat tgctgaactg
    16141 aaacggttgc atcgtcaggg gaaagacatc gggcagtaca tttacctgcg ccggtatgag
    16201 cgtagccgca agcacagtgc ggcgctgatg ctggctggta tgcagggatt ccgcgatctg
    16261 ttttccggta ccaatccggc gaaaaaactg ctgcgtgata ttggtttgaa actggccgac
    16321 acgcttcctg gcgttaaacc acaacttatc cgccaggcaa tgggattaaa cgatttgcct
    16381 gaatggctgc gttaaaaatt tctcttctgc tgtttatttg atacccatca cactttcatc
    16441 tcccggtttt ttcgccggga gattttcctc atttgaaata aactaatttc acctccgttt
    16501 tcgcattata ttttctaatg tcattatttt ttgatttagt gttttttgac atttttttag
    16561 ctcttaatat tgccttattc aaattagctt tctaatggca tcatctttat atagaaactg
    16621 gtgtgttttt tggtttttta ttctgtcgcg attttggcgt tttttaacca taagctaatg
    16681 tgatgatcaa ttttacctta tggttaacag tctgtttcgg tggtaagttc aggcaaaaga
    16741 gaacgattgc gttggggacc gggagtggct ccgatgctgg gtttcgtggt gataatttca
    16801 ccatgaaaag ttgtcagccc cgcttattca atgaggacaa gatggcacaa cagactcctt
    16861 tgtacgaaca acacacgctt tgcggcgctc gcatggtgga tttccacggc tggatgatgc
    16921 cgctgcatta cggttcgcaa atcgacgaac atcatgcggt acgtaccgat gccggaatgt
    16981 ttgatgtgtc acatatgacc atcgtcgatc tccgcggcag ccgcacccgg gagtttctgc
    17041 gttatctgct ggcgaacgat gtggcgaagc tcaccaaaag cggcaaagcc ctttactcgg
    17101 ggatgttgaa tgcctctggc ggtgtgatag atgacctcat cgtctactac tttactgaag
    17161 atttcttccg cctcgttgtt aactccgcca cccgcgaaaa agacctctcc tggattaccc
    17221 aacacgctga acctttcggc atcgaaatta ccgttcgtga tgacctttcc atgattgccg
    17281 tgcaagggcc gaatgcgcag gcaaaagctg ccacactgtt taatgacgcc cagcgtcagg
    17341 cggtggaagg gatgaaaccg ttctttggcg tgcaggcggg cgatctgttt attgccacca
    17401 ctggttatac cggtgaagcg ggctatgaaa ttgcgctgcc caatgaaaaa gcggccgatt
    17461 tctggcgtgc gctggtggaa gcgggtgtta agccatgtgg cttgggcgcg cgtgacacgc
    17521 tgcgtctgga agcgggcatg aatctttatg gtcaggagat ggacgaaact atttctcctt
    17581 tagccgccaa catgggctgg actatcgcct gggaaccggc agatcgtgac tttatcggtc
    17641 gtgaagccct ggaagtgcag cgtgagcatg gtacagaaaa actggttggt ctggtgatga
    17701 ccgaaaaagg cgtgctgcgt aatgaactgc cggtacgctt taccgatgcg cagggcaacc
    17761 agcatgaagg cattatcacc agcggtactt tctccccgac gctgggttac agcattgcgc
    17821 tggcgcgcgt gccggaaggt attggcgaaa cggcgattgt gcaaattcgc aaccgtgaaa
    17881 tgccggttaa agtgacaaaa cctgtttttg tgcgtaacgg caaagccgtc gcgtgattta
    17941 cttttttgga gattgattga tgagcaacgt accagcagaa ctgaaataca gcaaagaaca
    18001 cgaatggctg cgtaaagaag ccgacggcac ttacaccgtt ggtattaccg aacatgctca
    18061 ggagctgtta ggcgatatgg tgtttgttga cctgccggaa gtgggcgcaa cggttagcgc
    18121 gggcgatgac tgcgcggttg ccgaatcagt aaaagcggcg tcagacattt atgcgccagt
    18181 aagcggtgaa atcgtggcgg taaacgacgc actgagcgat tccccggaac tggtgaacag
    18241 cgaaccgtat gcaggtggct ggatctttaa aatcaaagcc agcgatgaaa gcgaactgga
    18301 atcactgctg gatgcgaccg catacgaagc attgttagaa gacgagtaac ggctttattc
    18361 ctcttctgcg ggagaggatc agggtgagga aaatttatgc ctcaccctca ctctcttcgt
    18421 aaggagagag gttcacaatt cactgcacgt ttcaggaacc atcgctcatg acacagacgt
    18481 taagccagct tgaaaacagc ggcgctttta ttgaacgcca tatcggaccg gacgccgcgc
    18541 aacagcaaga aatgctgaat gccgttggcg cacaatcgtt aaacgcgctg accggccaga
    18601 ttgtaccgaa agatattcag cttgcgaccc cgccgcaggt tggcgcaccg gcgaccgaat
    18661 acgccgcact ggcagaactc aaggctattg ccagtcgcaa taaacgcttc acgtcttaca
    18721 tcggcatggg ttacaccgcc gtgcagctac cgccggttat cctgcgtaac atgctggaaa
    18781 atccgggctg gtataccgcg tacactccgt atcaacctga agtctcccag ggccgccttg
    18841 aagcactgct caacttccag caggtaacgc tggatttgac tggactggat atggcctctg
    18901 cttctcttct ggacgaggcc accgctgccg ccgaagcaat ggcgatggcg aaacgcgtca
    18961 gcaaactgaa aaatgccaac cgcttcttcg tggcttccga tgtgcatccg caaacgctgg
    19021 atgtggtccg tactcgtgcc gaaacctttg gttttgaagt gattgtcgat gacgcgcaaa
    19081 aagtgctcga ccatcaggac gtcttcggcg tgctgttaca gcaggtaggc actaccggtg
    19141 aaattcacga ctacactgcg cttattagcg aactgaaatc acgcaaaatt gtggtcagcg
    19201 ttgccgccga tattatggcg ctggtgctgt taactgcgcc gggtaaacag ggcgcggata
    19261 ttgtttttgg ttcggcgcaa cgcttcggcg tgccgatggg ctacggtggc ccacacgcgg
    19321 cattctttgc ggcgaaagat gaatacaaac gctcaatgcc gggccgtatt atcggtgtat
    19381 cgaaagatgc agctggcaat accgcgctgc gcatggcgat gcagactcgc gagcaacata
    19441 tccgccgtga gaaagcgaac tccaacattt gtacttccca ggtactgctg gcaaacatcg
    19501 ccagcctgta tgccgtttat cacggcccgg ttggcctgaa acgtatcgct aaccgcattc
    19561 accgtttgac cgatatcctg gcggcaggcc tgcaacaaaa aggtctgaag ctgcgccatg
    19621 cgcactattt cgacactctg tgtgtggaag tggccgacaa ggcgggcgta ctggcgcgtg
    19681 ccgaagcggc tgaaatcaac ctgcgtagcg atatcctgaa cgcggttggg atcacccttg
    19741 atgaaaccac cacgcgtgaa aacgtaatgc agcttttcag cgtgctgctg ggcgataacc
    19801 acgggctgga gatcgacacg ctggacaaag acgtggctca cgacagccgc tctattcagc
    19861 ctgcgatgct gcgcgacgac gaaatcctca ctcatccggt gtttaatcgc taccacagcg
    19921 aaaccgaaat gatgcgctat atgcactcgc tggagcgtaa agatctggcg ctgaatcagg
    19981 cgatgatccc gctgggttcc tgcaccatga aactgaacgc cgccgccgag atgatcccaa
    20041 tcacctggcc ggaatttgcc gaactgcacc cgttttgccc gccggaacag gctgaaggtt
    20101 atcagcagat gattgcgcag ctggctgact ggctggtgaa actgaccggt tacgacgccg
    20161 tttgtatgca gccgaactcc ggcgcacagg gcgaatacgc gggcctgctg gcgattcgtc
    20221 attatcatga aagccgcaac gaagggcatc gcgatatctg cctgatcccg gcttctgcgc
    20281 acggaactaa ccccgcttct gcacatatgg caggaatgca ggtggtggtt gtggcgtgtg
    20341 ataaaaacgg caacatcgat ctgactgatc tgcgcgcgaa agcggaacag gcgggcgata
    20401 acctctcctg tatcatggtg acttatccct ctacccacgg cgtgtatgaa gaaacgatcc
    20461 gtgaagtgtg tgaagtcgtg catcagttcg gcggtcaggt ttaccttgat ggcgcgaaca
    20521 tgaacgccca ggttggcatc acctcgccgg gctttattgg cgcggacgtt tcgcacctca
    20581 acctgcataa aactttctgc attccgcacg gcggtggtgg tccgggtatg ggaccgatcg
    20641 gtgtgaaagc gcatctggca ccgtttgtac cgggtcatag cgtggtgcaa atcgaaggca
    20701 tgttaacccg tcagggcgca gtttctgcgg caccgttcgg tagcgcctct atcctgccaa
    20761 tcagctggat gtacatccgc atgatgggcg cagaagggct gaaaaaagca agccaggtgg
    20821 caatcctcaa cgccaactat attgccagcc gcctgcagga tgccttcccg gtgctgtata
    20881 ccggtcgcga cggtcgcgtg gcgcacgaat gtattctcga tattcgcccg ctgaaagaag
    20941 aaaccagcat cagcgagctg gatattgcca agcgcctgat cgactacggt ttccacgcgc
    21001 cgacgatgtc gttcccggtg gcgggtacgc tgatggttga accgactgaa tctgaaagca
    21061 aagtggaact ggatcgcttt atcgacgcga tgctggctat ccgcgcagaa attgaccagg
    21121 tgaaagccgg tgtctggccg ctggaagata acccgctggt gaacgcgccg cacattcaga
    21181 gcgaactggt cgccgagtgg gcgcatccgt acagccatga agttgcggta ttcccggcag
    21241 gcgtggcaga caaatactgg ccgacggtga aacgtctgga tgatgtttac ggcgaccgta
    21301 acctgttctg ctcctgcgta ccgattagcg aataccagta attcactgat tcgactattt
    21361 tctaaaggcg cttcggcgcc tttttagtca gatgacaaag tacaaaagtg ctcagacagt
    21421 cccctcgccc ctttggggag agggttaggg tgaggggaac aggccggcac tggcgcgaat
    21481 atttaccctc accccggccc tctccctgaa agggcgaggg gaaaagcgtg ccaacattga
    21541 agattgagcc agtttgttag caatctcaaa gatacgtcaa cgaattaatt tttctcggaa
    21601 aaacaaatgg ctatagcact tgtgaccggt ggcagtcgcg gcatcgggcg ggcaactgca
    21661 ttactgttgg cgaaagaaga gtatacggtg gcggttaatt atcaacaaaa cctccatgcg
    21721 gcgcaggaag tggtgaactt aatcacgcaa gccggtggca aggcattcgt gctccaggcg
    21781 gatatcagcg acgaaaatca ggtcatcgca atgtttacag caatcgatca gcacgttgaa
    21841 ccgctagcag cgctggtcaa taacgccggg atcttgttta cccagtgcac cgttgaaaac
    21901 cttaccgcag agcgaatcaa ccgagtactt tccaccaacg tgacgggata ttttctctgc
    21961 tgccgcgagg cggtaaaacg catggcgctt aaaaatggtg gcagtggcgg cgctatcgtc
    22021 aatgtctctt cggtggcctc acggttgggt tcgccagggg aataggttga ttacgcggca
    22081 tcgaaagggg cgattgatac gttaaccacc ggactatcgc tggaagtcgc cgcgcagggg
    22141 atccgcgtta actgcgtgcg gccagggttt atttataccg aaatgcacgc cagcggcggc
    22201 gagcttggac gcgtcgatcg cgttaagtcg aacatcccca tgcagcgtgg tggacaggca
    22261 gaagaggtcg cgcaggccat tgtctggcta ctaagtgata aagcctctta cgtcacggga
    22321 agttttatcg atttggcggg cgggaaataa aacagggaag ttgtctgacc ggatgcaaca
    22381 agtattgcat ccggtacttc atcgacttaa agcttctcgc cgttgctggc aatcacctct
    22441 ttgtaccagt taaagctctt cttacgtgaa cgcgacatat cgccagtacc gtcgtcatgt
    22501 ttattcacat agataaagcc gtagcgtttg ctgtactgcc cggtagtgaa tgacacgcag
    22561 tcgatgcagc cccacggtgt gtagcccatc agatccacac catcgtaagt cactgctttt
    22621 ttcatctctt caatatgggc gcgcaggtag tcaatgcggt agtcgtcgtt gatgctgcca
    22681 tcttcttcca ctttgtcgta agcgccaaaa ccgttttcga caataaacag cggcctctga
    22741 taacgctcat acagttcgca aagggcatag cgcagaccta ctggatcaat ctgccagccc
    22801 cagtcagacg ctttaacata cgggttcggt acgctgcctt caaaaccaga gatcgcatcg
    22861 ccggtgccgc cttcggcctt cactgcgttg atcatgtaat agctgaaacc aagataatcg
    22921 caggtgcctt cacgcagtac atccagatcg ccatcttcca ttttgatgtt aaatccgcga
    22981 cgctcccact cgttcaacac ataggacggg taatagccgc gtagctgcac atcggtaaag
    23041 acgtagcgtt cgcgcatcga ctcctgagcg aacatcacat cgtccgggtt acaggagtaa
    23101 ggatagagcg gcaccatcgc cagcatacag ccgactttca tctccgggtt aatgcgacgc
    23161 gcggctttca ctgctagggc gctggcgaca aactggtgat gcagcacctg gtacatcgtc
    23221 tcttccgggt tgtcatgctc ggtatacacc acgccggagc agcagtaacc aaacagcggc
    23281 gcacgccagt tacgctggtt gtttatttcg ttgaaggtca tccagtattt gactttatgc
    23341 ttgtagcgtt cgaataccac ttccgcgaaa cggacaaaga aatcaacgac tttacggttg
    23401 gtccagctac cgtattgctg caccagatgc agcggtattt caaagtggga gagggttatc
    23461 accggttcga tgttgtattt cagcagttca tcgaacagag agtcgtaaaa cttcagccct
    23521 tcttcgtttg gctgagcttc atcacctttt ggaaaaatac gcgtccaggc gatggaagtc
    23581 cgaaaacatt tgaagcccat ttcggcaaat agcttgatgt cttccttgta gtgaccataa
    23641 aaatcaacgg cttcatggtt tggatagtat tttcctggca agacttcttt ggtgatttcg
    23701 cgcggcacgc cgtgtgcgcc accggtcaga acgtcacaaa tgctcggtcc tttgccgcct
    23761 ttgttccagc cgccttcgac ctgatgagcg gcaacggcgc caccccataa gaaatctttc
    23821 ggtaaggtga gttttttcac tatcataggc tccagatgag tcaattctta caggaaatag
    23881 taccatgcag ccaaacgaca tcactttttc tcaacgtttc caggatgaca ttctggctgg
    23941 gcgtaaaacc atcaccatcc gcgacgagtc tgaatcgcac ttcaaaacgg gtgatgtgct
    24001 tcgtgtcgga cgttttgaag atgacggtta tttttgcacg attgaagtca ccgcaacctc
    24061 aaccgtaacg ctggatacgc tgacggaaaa acatgcagag caggaaaata tgaccctgac
    24121 tgaactgata aaggtcattg ccgacatcta tcccggtcag acacagtttt atgtgattga
    24181 atttaaatgt ctttaaattg aactcacgcc tgttagttga aatttctcat ttaatttcat
    24241 gatttttagg agattagtct caaatcagaa tttattttag ctaacaggtg ttcactggaa
    24301 ctattctcag ttacgctgga gaggtataca tgtgcgtgta cgtttctccg gagtaagtta
    24361 tggttcagaa gcccctcatt aagcagggat attcgctggc agaggaaatt gccaacagcg
    24421 tcagtcacgg cattgggttg gtgtttggta tcgttgggct ggtgttgcta ctggttcagg
    24481 cggtggatct caatgccagc gccacagcga taaccagcta cagcctctat ggcggcagta
    24541 tgatcctgct gttcctcgct tcgacgctct atcacgccat tcctcatcaa cgggcaaaaa
    24601 tgtggctgaa gaaatttgac cattgcgcta tttacctgtt gattgccgga acctacacgc
    24661 cgtttttgct ggtggggctg gattctccgt tagcgcgcgg gttgatgatt gttatctgga
    24721 gcctggcatt gctggggatt ctgtttaaac tgaccatcgc gcaccgattc aaaattttat
    24781 ctctggtgac ctatctggcg atgggctggc tgtcgctggt ggtaatttat gaaatggcag
    24841 ttaagctcgc ggcgggcagc gttaccttac tggcggtagg cggcgtggtt tattcgctcg
    24901 gagtgatttt ctatgtctgc aaacgcattc cctacaatca tgccatctgg cacggcttcg
    24961 tgctcggcgg cagtatgtgc cactttctgg cgatctattt gtatattggg caggcgtaat
    25021 gggtatgcct ttgtaggcct gataagacgc gccagcgtcg catcaggcat cggagcgcaa
    25081 ccgccggatg cggcgtgaac gccttatccg atctacgggc ctcgtccctg ttgtgataaa
    25141 ggcatcaaat cccagacatg ataagacgcg tcagcgtcgc atcaggcgat aaatgcctga
    25201 tgcgatacag agatttactc ttcgagtgaa tacggcagcg gctcgatatg cagtgtattc
    25261 gcatcgtcgc gtacgcggaa gatgctgtcc ggttccatat cgttattcat gacgacctgt
    25321 accacgacct gaccatcttc cagttttacc gcagccagca ccgtaccggt acgacgccag
    25381 ttctcgccca ttttcagctc taagtcttca ccagcttccg gcagtcggct ggcgctacct
    25441 gccagcaacc agagcgcacg tttattggca ccacggaatt ttgctcgcgc caccatctct
    25501 tgtccggtat aacagccttt cttaaaactg ataccgccca gcgcctggag attggtcgcc
    25561 tgtgggataa actgcccgct gttcgcggca tcaatcaccg ggaaacccgc ttcaatgttt
    25621 aatgccagcc actgttggct attgttcagt tccgcttcac cgcgcagttt atcggtcagc
    25681 atattggcag tagcttcatc ggttacgatc aggaaacgtt ctgccgggtg ttcaaaccat
    25741 agcaaagtgg tcgcgccttc tttaactacc tgtttttctc tcgaaggtag ttcactaaag
    25801 agattggcga gcgcggcgcg cgcctgaaaa ccggcaacac caagcagcac acgctcgtcg
    25861 tctgccgcga tggtcacttt agagaacacc gcatattttt tcagttcagt cagctgcggt
    25921 tcacgcacgc tgcgccgttc aatccacgca aagccatcgc cgtcgcggaa cagacgtaaa
    25981 ttgctccaca ttttaccttt ggcgtcgcaa tgggcggcga gcaggtgctg attttctgtc
    26041 atctggctga catctgctgt cacctgaccc tgcatatatt tttcgctgtc cgcgccagta
    26101 atggtggcaa gcgcccagtc atcaagcgtc atcagcgtca gcggtaaacg ggcagaagcc
    26161 gtaggctgac ggggaggaaa aggtgtaaaa gccataatag agtcctgagt tgcttaacgg
    26221 cgtattaata actaatggta aaagagctat gaggcaatgc aagtgctttc actacgcgat
    26281 ctgtgccttc tgcgaggcga cttccaaaaa aatgaagctc ttgaattgtt gtgaattaat
    26341 ttcgcgatcg cgatggtgtt tactgatatt cccgtaaaag aagtcgaaaa acacgttaca
    26401 atagccgggt actggatttt cgtgagacag gaagaagatg gacattaaca acaaagcccg
    26461 cattcattgg gcatgccgcc gtggtatgcg cgaactcgat atttcaatca tgccgttttt
    26521 cgaacatgag tacgacagct taagcgatga cgaaaaacgc atctttattc gtctgctgga
    26581 atgtgacgat ccggacctgt ttaactggct gatgaatcac ggtaaaccgg ccgatgcaga
    26641 actggaaatg atggtccgac tcatccagac acggaaccgg gaacgtggtc ctgtggcaat
    26701 ctgatttgcg cgtctcctgg cgcgcacagt ggctttcctt gctgattcat gggctggttg
    26761 ccgctgttat tttactcatg ccctggccgc tcagttacac cccgttatgg atggtgttac
    26821 tttcgctggt ggtatttgat tgcgttcgca gccagcggcg tattaatgct cgccaggggg
    26881 aaattcgatt gttgatggac ggacgtttgc gttggcaagg gcaggagtgg agcatcgtca
    26941 aagcaccgtg gatgattaag agcggcatga tgctgcgttt acgttctgat ggcggtaaac
    27001 ggcaacattt atggctggca gccgacagca tggacgaagc tgaatggcgg gatttacggc
    27061 ggattttgtt gcaacaagag acgcaaagat aaatatgacg gcaaagcata tgcgctaagc
    27121 cgtcagtgag atcaggcgta atgctctgcc atttcgttga ggatttgctc gcaccagctc
    27181 tgaatacgct cgtcgctaag gtcatactgg ttagtttcat ccagcgccag acccacgaac
    27241 agttgcccgt cagcaatcac cggtttcggg ctggtaaatt catatccttc cgttggccag
    27301 tagccgacga acttcacgcc tttggtcgag agtttgtcat gcagcatacc gagcgcatcg
    27361 aggaaccact cgccgtatcc cagttgatcg ccaagcccat acagcgcaac aattttacct
    27421 tcaaggttca ggtcgtcgag ctgatcccag acggcttccc agtcttcctg gatttcgcca
    27481 aaatcccagg tcgggatacc cagaatgagc acatcgtact gctccattaa tttcggggag
    27541 tcgtccttga ggttatgtaa ggtcaccagt tctgggccga taatatcgcg gattttttct
    27601 gccgccattt cggtgtaaca ggtgctggaa ccgtaaaaaa gacccatatt cataacgtaa
    27661 gcttctcgat tctgctgtgg cttttgcggc tagtgtatca gaatcgcttt agtcacggca
    27721 caataaggca taatggatca aatgagtgac cataaggggc gcaagtgaaa caggatctgg
    27781 cacgcatcga gcagtttctt gatgctctgt ggctggaaaa aaatctggct gaaaatacgt
    27841 tgaacgctta ccgtcgcgat ctgtcaatga tggtggagtg gttgcatcac cgcgggttga
    27901 cgctggcgac ggcgcaaagt gacgatttgc aggcattact ggcagaacgg ctagagggcg
    27961 ggtataaagc caccagctca gcgcgtttgc tgagtgcagt gcgccgattg ttccagtatc
    28021 tttatcgcga aaagtttcgt gaagacgatc ccagtgcgca tctcgcttca ccgaaattgc
    28081 cacagcgttt gccaaaagat ttaagcgaag cgcaggtcga acgtttatta caggcaccat
    28141 taattgatca gccactggag ctacgcgata aagccatgct tgaagtgttg tatgctaccg
    28201 ggctgcgtgt ctctgaactg gtcgggctga caatgagtga tatcagccta cgtcagggcg
    28261 tagtgcgggt cattggtaaa ggcaacaaag agcgcctggt gccgttaggt gaagaggccg
    28321 tttactggct ggaaacctat ctggaacatg ggcgtccgtg gctgttgaat ggtgtgtcaa
    28381 ttgacgtgtt gtttcccagc cagcgtgcgc agcagatgac gcgacagacc ttctggcacc
    28441 gcattaaaca ttatgccgtg ctggcgggta tcgacagcga aaagctgtca ccgcatgtgt
    28501 tgcgtcacgc ttttgccact catttattaa atcatggtgc ggatttacgc gtggtgcaga
    28561 tgctactggg ccacagcgat ctctccacca cgcaaattta tacgcatgtc gctaccgagc
    28621 gtctgcggca acttcatcaa cagcatcacc cgcgggcgtg atgtctgaaa agaacgggaa
    28681 gatttatgaa gaaaggtttt atgttgttta ctttgttagc ggcgttttca ggctttgctc
    28741 aggctgatga cgcggcaatt caacaaacgt tagccaaaat gggcattaaa agcagcgata
    28801 ttcagccagc acccgtggct ggcatgaaga ctgtgctgac taacagcggt gtgttgtaca
    28861 tcaccgatga cggtaaacat atcattcagg ggccaatgta tgacgttagt ggcgcggctc
    28921 cggtcaatgt caccaataaa atgctgctaa agcagttgaa tgcgcttgaa aaagagatga
    28981 tcgtttataa agcgccgcag gaaaaacacg tcatcaccgt gtttactgat attacctgtg
    29041 ggtactgcca caaactgcat gagcaaatgg cagactacaa cgcgctgggg atcaccgtgc
    29101 gttatcttgc tttcccgcgc caggggctgg agagccaggc tgagcaacaa atgaaagcca
    29161 tctggtgtgc gaaagataaa aacaaagcgt ttgatgatgt gatggctggt aaagctgcca
    29221 ctccggcaag ttgcgatatc gatattgccg atcattacgc acttggcgtc cagcttggcg
    29281 ttagcggcac tccggcaatt gtgctgagca atggcacact tgttccgggt taccagccgc
    29341 cgaaagagat gaaagaattc ctcgacgaac accaaaaaat gaccagcggt aattaattcg
    29401 cgtgaaacaa cagatacaac ttcgtcgccg tgaagtcgat gaaacggcag acttgcccgc
    29461 tgaattgcct cccttgctgc gccgtttata cgccagccgg ggagtacgca gtgcgcaaga
    29521 actggaacgc agtgttaaag gtatgttgcc ctggcagcaa ctaagcggtg tcgaaaaggc
    29581 cgttgagatc ctttacaacg cttttcgcga aggaacgcgg attattgtgg tcggtgattt
    29641 cgacgccgac ggcgcgacca gcacggctct aagcgtgctg gcgatgcgct cgcttggttg
    29701 cagcaatatc gactacctgg taccaaaccg tttcgaagac ggttacggct taagcccgga
    29761 agtggtcgat caggcccatg cccgtggcgc gcagttaatt gtcacggtgg ataacggtat
    29821 ttcctcccat gcgggggttg aacacgctcg ctcgttgggc attccggtta ttgttaccga
    29881 tcaccatttg ccaggcgaaa cattacccgc ggcggaagcg atcattaacc ctaacttgcg
    29941 cgactgtaat ttcccgtcga aatcactggc aggcgtgggt gtggcgtttt atctgatgct
    30001 ggcgctgcgc acctttttgc gcgatcaggg ctggtttgat gagcgcggca tcgcaattcc
    30061 taacctggca gaactgctgg atctggtcgc gctggggaca gtggcggacg tcgtgccgct
    30121 ggacgctaat aatcgcattc tgacctggca ggggatgagt cgcatccgtg ccggaaagtg
    30181 ccgtccaggg attaaagcgc tgctggaagt ggcaaaccgt gatgcacaaa aactcgccgc
    30241 cagcgattta ggttttgcgc tggggccacg tctcaatgct gccgggcgac tggacgatat
    30301 gtccgtcggt gtggcgctgt tgctgtgcga caacatcggc gaagcgcgcg tgctggcaaa
    30361 tgaactcgat gcgctaaatc agacgcgaaa agagatcgaa caaggaatgc agattgaagc
    30421 cttgaccctg tgcgagaaac tggagcgaag tcgcgacacg ctacccggcg ggctggcaat
    30481 gtatcacccc gaatggcatc agggcgttgt cggtattctg gcttcgcgca tcaaagagcg
    30541 ttttcaccgt ccggttatcg cctttgcgcc agcaggtgat ggtacgctga aaggttccgg
    30601 tcgctccatt caggggctgc atatgcgtga tgcgctggag cgattagaca cactctaccc
    30661 cggtatgatg ctgaagtttg gcggtcatgc gatggcggcg ggtttgtcgc tggaagagga
    30721 taaattcgaa ctctttcaac aacggtttgg cgagctggtt accgagtggc tggacccttc
    30781 gctattgcaa ggcgaagtgg tgtcagacgg cccgttaagc ccggccgaaa tgaccatgga
    30841 agtggcgcag ctgctgcgcg atgctggccc gtgggggcag atgttcccgg agccgctgtt
    30901 tgatggtcat ttccgtctgc tgcaacagcg gctggtgggc gaacgtcatt tgaaagtcat
    30961 ggtcgaaccg gtcggcggcg gtccgctgct ggatggtatt gcttttaatg tcgataccgc
    31021 cctctggccg gataacggcg tgcgcgaagt gcaactggct tacaagctcg atatcaacga
    31081 gtttcgcggc aaccgcagcc tgcaaattat catcgacaat atctggccaa tttagcgtca
    31141 tcttctctat aaaaaagagc gtggattggg tacaatcccg ctcttatcac cgcattttga
    31201 ctagctcaat aaaagaaatc agaccatgtt tgaaattaat ccggtaaata atcgcattca
    31261 ggacctcacg gaacgctccg acgttcttag ggggtatctt tgactacgac gccaagaaag
    31321 agcgtctgga agaagtaaac gccgagctgg aacagccgga tgtctggaac gaacccgaac
    31381 gcgcacaggc gctgggtaaa gagcgttcct ccctcgaagc cgttgtcgac accctcgacc
    31441 aaatgaaaca ggggctggaa gatgtttctg gtctgctgga actggctgta gaagctgacg
    31501 acgaagaaac ctttaacgaa gccgttgctg aactcgacgc cctggaagaa aaactggcgc
    31561 agcttgagtt ccgccgtatg ttctctggcg aatatgacag cgccgactgc tacctcgata
    31621 ttcaggcggg gtctggcggt acggaagcac aggactgggc gagcatgctt gagcgtatgt
    31681 atttgcgctg ggcagaatcg cgtggtttca aaactgaaat catcgaagag tcggaaggtg
    31741 aagtggcggg tattaaatcc gtgacgatca aaatctccgg cgattacgct tacggctggc
    31801 tgcgtacaga aactggcgtt caccgcctgg tgcgtaagag cccgtttgac tccggcggtc
    31861 gtcgccacac gtcgttcagc tccgcgtttg tttatccgga agttgatgat gatattgata
    31921 tcgaaatcaa cccggcggat ctgcgcattg acgtttatcg cgcgtccggc gcgggcggtc
    31981 agcacgttaa ccgtaccgaa tctgcggtgc gtattaccca catcccgacc gggatcgtga
    32041 cccagtgcca gaacgaccgt tcccagcaca agaataaaga ccaggccatg aagcagatga
    32101 aagcgaagct ttatgaactg gagatgcaga agaaaaatgc tgagaaacag gcgatggaag
    32161 ataacaaatc cgacatcggc tggggcagcc agattcgttc ttatgtcctt gatgactccc
    32221 gcattaaaga tctgcgcacc ggggttgaaa cccgcaatac gcaggccgtg ctggacggca
    32281 gcctggatca atttatcgaa gcaagtttga aagcagggtt atgaggaacc aacatgtctg
    32341 aacaacacgc acagggcgct gacgcggtag tcgatcttaa caatgaactg aaaacgcgtc
    32401 gtgagaagct ggcgaacctg cgtgagcagg gaattgcctt cccgaacgat ttccgtcgcg
    32461 atcatacctc tgaccaattg cacgcagaat tcgacggtaa agagaacgaa gaactggaag
    32521 cgctgaacat cgaagtcgcc gttgctggcc gcatgatgac ccgccgtatt atgggtaaag
    32581 cctcttttgt taccctgcag gacgttggcg gtcgtattca gctgtacgtt gcccgtgacg
    32641 atcttccgga aggcgtttac aacgagcagt tcaaaaaatg ggatctcggc gacatcctcg
    32701 gcgcgaaagg taaactgttc aaaaccaaaa ccggcgaact gtctatccac tgcaccgagc
    32761 tgcgtctgct gaccaaagca ctgcgtccgc tgccggacaa attccacggc ttgcaggatc
    32821 aggaagcgcg ctatcgtcag cgttatctgg atctcatctc taacgatgaa tcccgcaaca
    32881 cctttaaagt gcgttcgcag atcctctctg gcattcgcca gttcatggta aatcgcggct
    32941 ttatggaagt tgaaacgccg atgatgcagg tgatcccagg cggtgccgct gcgcgtccgt
    33001 ttatcaccca tcataacgcg ctggatcttg acatgtacct gcgtatcgcg ccggaactgt
    33061 acctcaagcg tctggtggtc ggtggcttcg agcgtgtatt cgaaatcaac cgtaacttcc
    33121 gtaacgaagg tatttccgta cgtcataacc cagagttcac catgatggaa ctctatatgg
    33181 cttacgcaga ttacaaagat ctgatcgagc tgaccgaatc gctgttccgt actctggcac
    33241 aggatattct cggtaagacg gaagtcacct acggcgacgt gacgctggac ttcggtaaac
    33301 cgttcgaaaa actgaccatg cgtgaagcga tcaagaaata tcgcccggaa accgacatgg
    33361 cggatctgga caacttcgac tctgcaaaag cgattgctga atctatcggc atccacgttg
    33421 agaagagctg gggtctgggc cgtatcgtta ccgagatctt cgaagaagtg gcagaagcac
    33481 atctgattca gccgaccttc attactgaat atccggcaga agtttctccg ctggcgcgtc
    33541 gtaacgacat taacccggaa atcacagacc gctttgagtt cttcatcggt ggtcgtgaaa
    33601 tcggtaacgg ctttagcgag ctgaatgacg cggaagatca ggcgcagcgc ttcctggatc
    33661 aggttgccgc gaaagacgca ggtgacgacg aagcgatgtt ctacgacgaa gattacgtca
    33721 ccgcactgga acacggctta ccgccgacag caggtctggg aattggtatc gaccgtatgg
    33781 taatgctgtt caccaacagc cataccatcc gcgacgttat tctgttcccg gcgatgcgtc
    33841 cagtaaaata agcattacgt tatgctaata accccggcaa atgtcggggt tttttaattg
    33901 tgctgcgcga aagcagacaa caattttctt gcttcactat tggctgcctg cattaccatc
    33961 cacggactga acgcccacgg cgtggcatca ataccgtgta atacatctgc taaatcacac
    34021 cattgataat ccatcacttc atcatcgttg atctgtaacg cactgttggt gcgtgcggca
    34081 aataccggac acacttcatt ttccacaatg ccattcggat cggtggcgcg atagcgaaag
    34141 tcaggataga tagattcagg aggcgtaatt tccacgccaa gctcataacg gcaacggcgg
    34201 atcactgcgt cttcgttgct ttctcccagt tgtgggtgcc cacaaaccga gttagtccac
    34261 acgccaggcc atgctttttt gctcagtgcg cggcgggtaa ctaataattg ccccttggca
    34321 ttaaacagcc aactggagaa cgcgagatgt aagagggtgt ctgccgtgtg tgcggcatac
    34381 ttttccagcg tacccgtggg aactccctgt gcattcaata aaatgacgtg ttccgtttgc
    34441 ataatttctc acatgtaatt ctgatcactc atcattatag ataattgaag agtgaaatga
    34501 ttaaccaagt gagcgtttat cgacaaccgc ccgttttgag cggatgccga caggtaaaaa
    34561 ccatttaaaa aagtagcatc atatatttga agcgactttc aatggccatt gcaacacagt
    34621 cagaaatgac aggaatagag agagtaaaaa gcaacagcag actgaatata gcgcgcatta
    34681 aaagatttat attattctcc atgctcattt ttcttcagct tgcgcatcag aagccacata
    34741 cgtactgtcc tgacttttaa atctttatca gagacggatt ccttcaccag gtcagcatga
    34801 tgctgatagc cattaaagaa gacgttcagc acaacggcgc taagggttgc gagcataatg
    34861 ccgctatgta gcagcggttg cagtacggcc ggtaacttag aaaagaaatc gtgagagagc
    34921 gtcggagtca tgccaacgcc gagactgatc gccacaatat agaggttgta acggttggtg
    34981 gtgtagttac agcgcgacag aattcgaatc cctgtcgcca gtaccatgcc gaacatcact
    35041 agaccagcgc cgcccagcac aaattgcgga atggaggcta ccagcaccgc catttttggc
    35101 accatgccaa acaggattaa tataattccc gaagaaatac acacccagcg gctatgaacg
    35161 cgcgtcacgc taaccaggcc aacgttttga gaaaatgacg tgtgggggaa gctgttaaac
    35221 gtgccgccta tcattgtccc tacgccatcg acacgcagcc cgcgaataat atcgtgtgaa
    35281 gagagtttac gaccgactat ttcacccagt gccaggaaca tccccatcga ctcgataaac
    35341 acgatgatta acacggcagt catggtcaga atggaaacgg gatcgaaaat cggcatacca
    35401 aacgacatcg gggtgacaat cgcaaaccac aaggcatcat gtagcccgga taaattgact
    35461 tcattcatca tccacgaaag taaaaagcca aatacaatcc ccagtaatac ggcgacgttg
    35521 gacataaatc ctttcgcata gcgagtaatg agcaagataa aaattaagac ggcaaaggag
    35581 atacctgaat aaacgggatt accatattgc ggattccctt tacctccggc ggcccagtca
    35641 atacccacct gaatgatgct aagtccgaca gaagtaataa ccacaccggt aaccagtggc
    35701 gggaataaag gcatcaagcg accgataagt ggcgctaata atgtggtgat aaaacccgcg
    35761 gcgatagtgg caccaaatat ccccagcagg ccaatatccg ggttcatccc aatggctatc
    35821 attggtgtta cagcagcaaa ggtcaccgac ataatcaccg gcaggcggat ccccataaag
    35881 cggccgatac cgatacattg caataatgtg acgatcccgc agcaaaagag atccgagcta
    35941 atgagcatcg caatagcttc ttttgagagg cccagtcggt caccaatcat aagaggaaca
    36001 gcgactgcac ctgcgtacat gacaaggacg tgttgcagac cgagtatgat cagctttcct
    36061 ggtgataata tgcggtcaac ctcatcagtt gggcggtctt gcccagaaga tgagagaagt
    36121 tgggaatcta tggcgctcat cagtgtttct cctttcgccc gccagagtaa aaagcaaccg
    36181 tttacgttaa tgaaactgtc taaacacatg ctcgtggagt gccagttttt gcgaggaagg
    36241 ggagttagag acaggaattg cggaggcaat cacataatcg tggttaaacc acgaaaatga
    36301 gaagagaacc tgacaatgga attatctgta attttctcat attgatattt cattaatcat
    36361 tctggcatgt cgtactctcg catttaatcg tttttatctg gatagcactc ttttgatcgg
    36421 caatgttgaa ataatgaaag cggcatagat tgaggggcaa taatttgaac tcagtcgcaa
    36481 aattataatt aaaacggtca tgctacctaa tatatgcccg tt
//
LOCUS       DADLFA010000018        36208 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000018 DADLFA010000000
VERSION     DADLFA010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36208
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.018"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(50..1699)
                     /gene="actP"
                     /locus_tag="JQH65_000779"
     CDS             complement(50..1699)
                     /gene="actP"
                     /locus_tag="JQH65_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HAY6138277.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGMLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGTMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1696..2010)
                     /locus_tag="JQH65_000780"
     CDS             complement(1696..2010)
                     /locus_tag="JQH65_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HAY6138278.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2142..4101)
                     /gene="acs"
                     /locus_tag="JQH65_000781"
                     /pseudo
     CDS             complement(2142..4101)
                     /gene="acs"
                     /locus_tag="JQH65_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006816721.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
     gene            4494..5930
                     /gene="nrfA"
                     /locus_tag="JQH65_000782"
     CDS             4494..5930
                     /gene="nrfA"
                     /locus_tag="JQH65_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HAY6138279.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQGFIKTVIPQWEEQARKNGLLSQ"
     gene            5975..6541
                     /gene="nrfB"
                     /locus_tag="JQH65_000783"
     CDS             5975..6541
                     /gene="nrfB"
                     /locus_tag="JQH65_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HAY6138280.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITAIPAAQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6538..7209
                     /gene="nrfC"
                     /locus_tag="JQH65_000784"
     CDS             6538..7209
                     /gene="nrfC"
                     /locus_tag="JQH65_000784"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HAY6138281.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7206..8162
                     /gene="nrfD"
                     /locus_tag="JQH65_000785"
     CDS             7206..8162
                     /gene="nrfD"
                     /locus_tag="JQH65_000785"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HAY6138282.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLAQQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKSWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8182..9900
                     /locus_tag="JQH65_000786"
     CDS             8182..9900
                     /locus_tag="JQH65_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HAY6138283.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQCALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPERRFYEARRQ
                     QMMEPSIRWNGIHDWYVVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            9893..10276
                     /gene="nrfF"
                     /locus_tag="JQH65_000787"
     CDS             9893..10276
                     /gene="nrfF"
                     /locus_tag="JQH65_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HAY6138284.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10273..10869
                     /gene="nrfG"
                     /locus_tag="JQH65_000788"
     CDS             10273..10869
                     /gene="nrfG"
                     /locus_tag="JQH65_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HAY6138285.1"
                     /translation="MKEPKIPVKMLTTLTILMVFLCVGSYLLSPKWQAVHAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11210..12523
                     /gene="gltP"
                     /locus_tag="JQH65_000789"
     CDS             11210..12523
                     /gene="gltP"
                     /locus_tag="JQH65_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HAY6138286.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12714..12968)
                     /locus_tag="JQH65_000790"
                     /pseudo
     CDS             complement(12714..12968)
                     /locus_tag="JQH65_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005118502.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
     gene            complement(12953..13402)
                     /locus_tag="JQH65_000791"
     CDS             complement(12953..13402)
                     /locus_tag="JQH65_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005045755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAY6138287.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPGSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLEKPRKIWRTTLPSMPKCCLV"
     gene            complement(13496..15643)
                     /gene="fdhF"
                     /locus_tag="JQH65_000792"
     CDS             complement(13496..15643)
                     /gene="fdhF"
                     /locus_tag="JQH65_000792"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(15224..15226),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HAY6138288.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIMANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGEQGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGSCNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15841..17307)
                     /gene="mdtP"
                     /locus_tag="JQH65_000793"
     CDS             complement(15841..17307)
                     /gene="mdtP"
                     /locus_tag="JQH65_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HAY6138289.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPSLGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDMIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIHSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17304..19355)
                     /gene="mdtO"
                     /locus_tag="JQH65_000794"
     CDS             complement(17304..19355)
                     /gene="mdtO"
                     /locus_tag="JQH65_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HAY6138290.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPA
                     AKPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDLLHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSCSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19355..20386)
                     /gene="mdtN"
                     /locus_tag="JQH65_000795"
     CDS             complement(19355..20386)
                     /gene="mdtN"
                     /locus_tag="JQH65_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HAY6138291.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDVLVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPNDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20405..20680)
                     /locus_tag="JQH65_000796"
     CDS             complement(20405..20680)
                     /locus_tag="JQH65_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6138292.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRIIQRANINLAFVGIIYTALFAVYAMLFWLAFF"
     gene            complement(20889..22874)
                     /gene="yjcS"
                     /locus_tag="JQH65_000797"
     CDS             complement(20889..22874)
                     /gene="yjcS"
                     /locus_tag="JQH65_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HAY6138293.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSHRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVRGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVISLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQPQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            23127..23444
                     /locus_tag="JQH65_000798"
     CDS             23127..23444
                     /locus_tag="JQH65_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase"
                     /protein_id="HAY6138294.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            23513..23842
                     /gene="yjdP"
                     /locus_tag="JQH65_000799"
     CDS             23513..23842
                     /gene="yjdP"
                     /locus_tag="JQH65_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
                     /protein_id="HAY6138295.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(23989..24747)
                     /gene="phnP"
                     /locus_tag="JQH65_000800"
     CDS             complement(23989..24747)
                     /gene="phnP"
                     /locus_tag="JQH65_000800"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HAY6138296.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECVACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGLHDLADRWPPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRSHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEAGFDGMEIGVA"
     gene            complement(24749..25198)
                     /gene="phnO"
                     /locus_tag="JQH65_000801"
     CDS             complement(24749..25198)
                     /gene="phnO"
                     /locus_tag="JQH65_000801"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HAY6138297.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PSMRYPSMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQACGLNVGSKL
                     LAWAEEEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            complement(25185..25742)
                     /gene="phnN"
                     /locus_tag="JQH65_000802"
     CDS             complement(25185..25742)
                     /gene="phnN"
                     /locus_tag="JQH65_000802"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HAY6138298.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEREFFTRAGQNLLALSWHANGLYYGVGIEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLKNRGRENTSEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            complement(25742..26878)
                     /gene="phnM"
                     /locus_tag="JQH65_000803"
     CDS             complement(25742..26878)
                     /gene="phnM"
                     /locus_tag="JQH65_000803"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HAY6138299.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLNAVAIGDVR
                     DGGDQLENLEKMINTIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARQIALASHDDATHAHVAESHQLGSVIAEFTTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVRLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRQGNHIHIDHVWRQGKRVF"
     gene            complement(26875..27555)
                     /gene="phnL"
                     /locus_tag="JQH65_000804"
     CDS             complement(26875..27555)
                     /gene="phnL"
                     /locus_tag="JQH65_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HAY6138300.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            complement(27666..28424)
                     /gene="phnK"
                     /locus_tag="JQH65_000805"
     CDS             complement(27666..28424)
                     /gene="phnK"
                     /locus_tag="JQH65_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HAY6138301.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYESRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            complement(28421..29266)
                     /gene="phnJ"
                     /locus_tag="JQH65_000806"
     CDS             complement(28421..29266)
                     /gene="phnJ"
                     /locus_tag="JQH65_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HAY6138302.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            complement(29259..30317)
                     /gene="phnI"
                     /locus_tag="JQH65_000807"
     CDS             complement(29259..30317)
                     /gene="phnI"
                     /locus_tag="JQH65_000807"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HAY6138303.1"
                     /translation="MYVAVKGGEKEIDAAHALQESRRRGNTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQSPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIMPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHYVD
                     FQAELELLKRLQQEQNHG"
     gene            complement(30317..30901)
                     /gene="phnH"
                     /locus_tag="JQH65_000808"
     CDS             complement(30317..30901)
                     /gene="phnH"
                     /locus_tag="JQH65_000808"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HAY6138304.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLSAPLSNDIVSQSLRFHTNAPLVNQSELATFAVTDEAIS
                     SEQLNALSSGTAVAPEAGATLILQVVSLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            complement(30898..31350)
                     /gene="phnG"
                     /locus_tag="JQH65_000809"
     CDS             complement(30898..31350)
                     /gene="phnG"
                     /locus_tag="JQH65_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HAY6138305.1"
                     /translation="MHADIATRQHWMSMLAHSQPAELAARLNALNITANYEVIRTAET
                     GLVQIQPRMGGTGERFFAGDATLTRAAVRLTDGPLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            complement(31351..32076)
                     /gene="phnF"
                     /locus_tag="JQH65_000810"
     CDS             complement(31351..32076)
                     /gene="phnF"
                     /locus_tag="JQH65_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HAY6138306.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPTEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGIALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            complement(32097..32876)
                     /gene="phnE"
                     /locus_tag="JQH65_000811"
     CDS             complement(32097..32876)
                     /gene="phnE"
                     /locus_tag="JQH65_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HAY6138307.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFVADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            complement(33002..34018)
                     /gene="phnD"
                     /locus_tag="JQH65_000812"
     CDS             complement(33002..34018)
                     /gene="phnD"
                     /locus_tag="JQH65_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY6138308.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            complement(34043..34831)
                     /gene="phnC"
                     /locus_tag="JQH65_000813"
     CDS             complement(34043..34831)
                     /gene="phnC"
                     /locus_tag="JQH65_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HAY6138309.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRAHTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            complement(34964..35407)
                     /gene="yjdN"
                     /locus_tag="JQH65_000814"
     CDS             complement(34964..35407)
                     /gene="yjdN"
                     /locus_tag="JQH65_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HAY6138310.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            complement(35567..35902)
                     /locus_tag="JQH65_000815"
     CDS             complement(35567..35902)
                     /locus_tag="JQH65_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004856597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HAY6138311.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDTEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
BASE COUNT         7832 a         9707 c        10224 g         8445 t
ORIGIN      
        1 ctggagagga agagtattcc ccggtcaggc gaccggggaa ggggagagat taatgtgcgc
       61 ggccttgctc aacgccaaag ccggtctggg agcggataaa ctgcgcacgg aacagttcac
      121 gctcgcgcgc accttccgct gagttatcgg ttgccgagaa gaaccagatg ccgaggaatg
      181 ccacgctgat agagaacagc gccgggtatt catacgggaa gatggctttt tcgtgaccga
      241 ggatctgtac ccaaatagtc gggccgagga tcatcagcac cactgcggta atcaacccca
      301 gccagccgcc cagcatcgtg ccacgggtag tcagtttcga ccagtacatg gaaagcagaa
      361 tgatcgggaa gttacagctc gccgcgatgg caaacgcaag gcctaccata aaggcgatgt
      421 tctgattctc aaacagcatg ccgaggataa tcgcaatcac acccaaaatc aggacggtga
      481 ttttcgatac ccgcagttct tcacgttcgg tcgcaccttt tttgaagacg ttagcgtaca
      541 agtcatgcga aaccgcggat gcgcccgcca gcgtcagacc cgcaaccacc gcgaggatag
      601 tggcgaaagc aaccgctgaa ataaaaccga ggaacaggtt gccgcccact gcattcgcca
      661 ggtgaacggc cgccatgttg ttaccaccaa tcagatggcc cgccgcgtct ttatattccg
      721 gattcgcacc aaccagcatg atcgcgccga agccgataat aaaggtcaga atatagaagt
      781 agcccataaa cccggtggcg tagaacacgc tcttacgtgc ttcgcgggca tcgctgactg
      841 taaagaagcg catcagaatg tgcggcaagc ccgccgtacc aaacatcagt cccagaccca
      901 gagagagcgc ggagatcgga tctttcacca gcccgcccgg cttcatgatg tcgacacctt
      961 tcgggtgtac cgccatcgct tcgctgaaca gattattgaa gctaaagccg acgtgtttca
     1021 tcaccataaa ggccataaag ctggcaccga acagcaacag cacggcttta ataatttgca
     1081 cccaggtggt cgccagcatg ccgccgaaca ggacgtacat catcatcagc acgccgacca
     1141 gcaccaccgc aatgtgatag ttaaggccaa acagcagctc gatcagttta cctgcgccta
     1201 ccatctgggc gataaggtaa agcgccacca ccaccagaga accacaggcc gaaagaatac
     1261 gaatcggccc ttgtttcagg cggtaagagg ctacatcggc aaaggtgtag cgtcccaggt
     1321 tacgcagacg ttcggcaatc aggaacaaaa tgatcggcca gcccaccagg aagcccagcg
     1381 agtaaattaa gccgtcatag ccggaggtaa acaccagcgc ggagatcccc aaaaatgagg
     1441 cggcggacat atagtcaccg gcaatcgcca gcccgttctg gaagcctgtg atattgccgc
     1501 ctgccgtgta gtagtcgcta cgagaacgta cgcgttttga tgcccagtag gtaatgccga
     1561 gcgtaaacac gacgaaaatc aggaacataa taatcgcctg ccagttcgtt ggctggcgct
     1621 ctacggcccc gctaatagca tccgcggcgt tagctgcgaa agggagtgtg gcggcaagcg
     1681 ccgtcagaac tcttttcatg atgcttgtac ctcatgcagg acttcattat taagacggtc
     1741 gaattcgccg ttcgcccgcc agatgtagat accggtgaga acaaaggaga tcacaatcac
     1801 tccaacacca attggaatac cgcgtgtgac gctggtgttc ggattcagcg gcgtgcccag
     1861 ccagccgggc gcgaaggcga tcagtaaaat aaagccgata taaactgcca gcataataat
     1921 cgacaggatg gtggcaaacc gttgccgttt ttcgactaac tccctgaaat gcgcattgtc
     1981 ttctatccgc tgataaatag tgccattcat cacagattct ccagaggtaa tgtagggatt
     2041 gttttaattc ccgctccctt atgggagaag gttaacgctc gggtaaccct tgccgaatgt
     2101 aggccggata aggcgtttac gccgcatccg gcaattgtgg gttacgatgg catcgcgata
     2161 gcctgcttct cttcaagcag cttctcgact acgccaggat cggcaagcgt cgaggtatcg
     2221 cccaggttgc tggtatcgcc cgccgcaatt ttgcgcagaa tacggcgcat aattttgccg
     2281 gagcgggttt taggcaggga gtcggtccag tgcagcacgt ctggcgtcgc cagcgggcca
     2341 atctctttac gcacccagtt gcggacttct gcgtacagtt ctggtgacgg ttcctccccg
     2401 tgattaagcg tgacgtaggc gtagatcgcc tgacctttaa tattgtgcgg aatacctact
     2461 acggcggctt cggcaatctt cggatgcgcc accagcgccg actcaatctc tgccgtcccc
     2521 agacggtgac cggagacgtt cagcacgtcg tccacacgcc cggtgatcca gtaatagcca
     2581 tcttcatcgc gacgcgcgcc gtcgccgctg aaatacatat ttttgaaggt ggagaagtag
     2641 gtctgttcaa aacgttcgtg atcgccaaac agcgtacgcg cctgacccgg ccaggagtcg
     2701 gtgatcacca ggctgccttc ggtagcgcct tccagcgggt taccttcgtt atcgaccagc
     2761 gccggttgca cgccgaagaa cggacgtgtt gccgaaccgg ctttcagccc ggtagcgcca
     2821 ggcagtggcg tgatcatgaa accgccagtt tcggtctgcc accaggtatc gaccaccgga
     2881 catttctcgt tgccgatttt tttccagtac cactcccacg cttccgggtt aattggctcg
     2941 cccacggaac agagaatgcg cagcgacgaa cggtcggtac cctcgatcgc tttatcgcct
     3001 tccgccatca gcgcgcggat cgccgtgggt gcggtataga gaatattgac ctgatgcttg
     3061 tccaccacct gcgccatacg ggcaggcgtc ggccagttgg gtacgccttc aaacatcagc
     3121 gtggtcgcac cgcaggccag cgggccgtac agcaagtaac tgtgtccggt cacccagccc
     3181 acatcggcgg tgcaccagta gatatcaccc ggatgataat caaagacata tttaaaggtc
     3241 agcgccgcgt acaccagata accgccggta gtatgcagca caccttttgg cttaccggta
     3301 gaaccggagg tgtagagaat aaacagcgga tcttcggcgt tcatctcttc cgcctggtgc
     3361 tgatcgctcg cttgctcaac caggtcgtgc caccacaggt cgcgcccttc ctgccagtca
     3421 attttcccgc cagtacgctt cagtaccacc acatgctcta cgctggtgac gttcgggttt
     3481 ttcagcgcgt catcaacgtt tttcttcagc ggaatactgc cgcccggcac gcacaccttc
     3541 gtcggaagtg atcaccagtc gtgagttgga atcaataatg cgcccggcaa cggcttccgg
     3601 cgagaagccg ccgaaaatca ccgaatgcac cgcgccaatg cgggcgcagg ccagcatcgc
     3661 aaccgcggct tccggcacca tcggcatata aatcgccacc acatcacctt ttttaatgcc
     3721 cagctcgagc agggtattgg cgaagcggca gacgtcgcgg tgcagctctt tatagctgat
     3781 atgtttgctc tggctggcgt cgtcgccttc ccagatgatg gcggtacgat cgccgttttc
     3841 ttgcagatgg cggtcaaggc agtttgccgc cagattcagc gtgccgtcct cgtaccattt
     3901 aatggacaca ttaccggggg caaaggaggt gtttttcacc ttctggtaag gtttgatcca
     3961 gtcaagaatt tttccctgtt cgccccagaa ggtatcaggt acgttaatag attgttgata
     4021 cattgcctcg tactgctgag ggtttatcag gcaacggtct gcgatgttgg caggaatggt
     4081 gtgtttgtga atttggctca tgcttttgtt ctccttgtag aatgttaata atatgtgaca
     4141 taagcgttaa atgtaggggt attggcagtt ttgtttagta tttgggcgac agatcacgca
     4201 aaagtagaat tgtgcaaata aacggcaggg taatttttga aggtcagcaa caaaagttga
     4261 ttaattcttt gaggaacatg cagttatgca tgctgttgaa aaagaggaag atactgacta
     4321 actctaaagt ggtattttac atgcacttac aattgattaa agacaacatt cacagtgtgg
     4381 ttatttgtta cacatagggg cgagcaatgt catgacagtg taggtgcggt tactgtcgtg
     4441 aaaaacaata aaaaccgcca ttgcaacaat ggcgcaattc ggatgaagcc cctatgacaa
     4501 ggataaaaat aaacgcacgc cgtatcttca gcttattgat tccttttttc tttttcactt
     4561 ctgttcacgc tgaacaaacg gcagctcccg caaaacctgt aactgtggaa gcgaagaatg
     4621 aaacctttgc cccgcagcat cccgatcaat atctctcctg gaaagccacc tcggaacagt
     4681 cagagcgtgt tgacgccctg gcggaagatc cacggctggt gatcctgtgg gcggggtatc
     4741 ccttctcgcg cgattacaac aagccgcgtg gacatgcctt tgctgtgacc gatgtgcgtg
     4801 aaaccctgcg taccggtgcg ccgaaaaatg ctgaagatgg tccgctaccg atggcgtgct
     4861 ggagttgtaa aagcccggat gtggcgcgtc tgatccagaa agacggcgaa gatggctact
     4921 tccacggtaa gtgggcgcgc ggcggcccgg aaatcgtcaa caacttaggt tgtgccgact
     4981 gccataacac cgcctcacca gagttcgcca aaggcaaacc ggagttaacc ctttcccgtc
     5041 cgtatgcggc tcgcgcgatg gaagccattg gtaaaccttt tgagaaagcc ggacgtttcg
     5101 accagcaatc gatggtttgc ggtcagtgcc atgtggagta ttacttcgac ggcaaaaaca
     5161 aagcggttaa attcccgtgg gatgacggca tgaaagtcga aaatatggag cagtattacg
     5221 acaaaattgc cttctctgac tggactaact ccctgtcgaa aacgccaatg ctgaaagcgc
     5281 agcacccgga atatgaaacc tggacagcgg gcattcacgg taaaaacaac gtgacctgta
     5341 tcgactgcca tatgccaaaa gtgcagaacg ccgaaggcaa actctacacc gaccataaaa
     5401 ttggtaatcc gtttgataac ttcgcccaga cttgtgcgaa ctgccatacc caggacaaag
     5461 ctgccttgca aaaagtggtc gcggaacgta agcagtcgat taacgacctg aaaatcaagg
     5521 ttgaagatca actggttcac gctcacttcg aagcgaaagc agcgctggat gcaggcgcga
     5581 cggaagctga aatgaagcca attcaggacg atatccgtca tgcccagtgg cgctgggatc
     5641 tggcgatcgc ttcccacggc attcatatgc acgcaccgga agaaggttta cggatgctcg
     5701 gtacggcgat ggataaagcg gcggatgcac gcaccaaact ggcgcgcctg ctggcgacca
     5761 aaggcatcac ccatgaaatc cagatcccgg atatctcaac caaagagaaa gcccagcagg
     5821 ccattggcct gaacatggaa caaatcaagg ccgagaagca gggcttcatc aaaacggtga
     5881 tcccgcagtg ggaagaacag gcacgtaaaa acggtctgtt aagccaataa ccccgttccg
     5941 cctcgcaagg ggcggaaaac acaatggagt gaatatgagc gtattacgtt cgttattaac
     6001 tgctggggtg ctggcgtcgg gcctgttgtg gagcctgaac ggaattaccg ctatccctgc
     6061 ggcgcaggca tctggtgatc gctacgaagt tacccagcag cgtaacccgg atgccgcctg
     6121 tctggactgt cataaaccag ataccgaagg tatgcatggc aaacatgctt ccgttatcaa
     6181 cccgaataac aaattgccgg tcacctgcac caactgtcac ggccagccat caccgcaaca
     6241 ccgcgaaggg gtgaaagatg tgatgcgctt taacgagccg atgtacaagg tcggagagca
     6301 gaacagcgtc tgtatgtcct gtcatctgcc ggaacagttg caaaaagcgt tctggccgca
     6361 cgatgtccac gtaaccaaag tggcgtgcgc cagctgccat tccctgcatc cacagcaaga
     6421 tacgatgcag acgttaagtg acaaaggacg gattaagatt tgcgtcgatt gccacagcga
     6481 tcagcgcacc aatccgaact ttaacccggc gtctgttccg ttgcttaagg agcagccatg
     6541 acctggtctc gtcgccagtt tctcaccggc gtcggcgtgc tggcagccgt cagcggcacc
     6601 gcagggcgcg tggtggcgaa aacgttgaat atcaatgggg tgcgttacgg catggtgcat
     6661 gacgagtcgt tatgcatcgg ctgtaccgcc tgtatggatg cttgtcgaga agtgaacaag
     6721 gtgccggaag gcgtctcgcg cctgacgatt attcgtagcg agccgcaggg cgaatttcct
     6781 gatgtgaaat atcgcttctt ccgtaagtct tgccagcact gcgatcatgc gccgtgcgtt
     6841 gacgtctgcc cgaccggtgc gtcttttcgt gatgccgcta gcgggattgt cgatgttaat
     6901 cctgatctct gcgtcggttg tcagtactgc atcgccgcct gtccgtaccg cgtgcgcttt
     6961 atccatccgg tcacgaagac ggcggacaaa tgcgatttct gccgtaaaac caatttgcag
     7021 gccggtaagc tgcccgcgtg cgttgaggct tgcccgacga aggcgctgac gtttggcaat
     7081 ctggacgatc ctaacagtga gatttcgcaa ctgctgcgcc agaagcctac ttaccgctac
     7141 aagctggcgc tgggaaccaa accgaagctg taccgcgtac cgtttaaata cggggaggtg
     7201 agccaatgac gcagacttcc gcatttcatt ttgaatcgct ggtgtgggac tggccgattg
     7261 ccatctacct gtttttgatt ggtatttctg ccggtctggt gacgctggcc gtgctgttac
     7321 gtcgcttcta cccgcaggcg ggcggtgcag acagtacgtt gctgcgcacc acgctgattg
     7381 tcgggccggg cgcggtgatc ctcggtctgt tgatcctcgt cttccacctg acaagaccgt
     7441 ggaccttctg gaagctgatg ttccactaca gttttacctc ggtgatgtcg atgggggtga
     7501 tgctgtttca gctttacatg gtggtgctgg tgctgtggct ggcgaaaatc tttgaacatg
     7561 atttgcttgc ccagcaacaa cgctggttgc cgaagctggg gatcgtgcaa aaggttctga
     7621 gcctgctgac gcccgttcat cgcggactgg aaacgctgat gctggtgttg gcggtgctgc
     7681 tgggggctta taccggcttt ctgctgtcgg cgctgaaatc gtatccgttc ctcaataacc
     7741 cgatcctgcc ggtgctgttc ctcttctccg gcatctcgtc cggtgcggcg gtggcgctga
     7801 tcgccatggc gatacgccaa cgcagtaacc cgcattccac ggaagcgcac tttgttcacc
     7861 ggatggaaat ccccgtggta tggggtgaaa tcttcctgct ggtggcgttt tttgtcggtc
     7921 tggcgctggg cgatgacggt aaagttcgtg cgctggtggc ggcattaggt ggcggtttct
     7981 ggacgtggtg gttctggctt ggtgtcgccg ggctggggct gattgtgcca atgttgctca
     8041 aatcgtgggt caatcgcagt tccggcattc ctgccgtgct ggcggcgtgt ggggccagtc
     8101 tggtcggcgt gttgatgctg cgctttttca ttctctacgc cggacagttg acggtggcgt
     8161 aagccagaaa agaggtggtt tctggacgta ttccttcctg aagtcggttt tctggcgttg
     8221 ttgttaagtc tcggggtcaa cgtgttgacc ccgttgacgg cctttgcggg agtgcggttg
     8281 cgctggcctg ccatgatgcg actcacttgc atcggcattc tggcgcagtg cgcgctcctg
     8341 ctgctcgcct ttggcgtact gacgtattgt tttctcatca gcgatttctc ggtcatttat
     8401 gtcgcccaac atagctacag cctgctgtcg tgggaactca aactggcggc ggtgtggggc
     8461 ggtcatgaag gttcgctgct gctttgggtg ctgctgcttt ccgcctggag cgcgctgttt
     8521 gcctggcatt atcggcagca aaccgatccg ctatttccgc tgacgctagc cgttttatct
     8581 ctcatgctcg ccgcactgct actgtttgtg gtgctgtggt ccgatccctt cgtgcggata
     8641 tttccaccag caatcgaagg ccgcgatctc aatccgatgc tgcaacatcc cggtcttatc
     8701 tttcatccac cgctgcttta ccttggctat ggcggtttga tggtagcggc gagcgtggcg
     8761 ctggcgagtc tactgcgcgg cgagtttgat gcggccagcg cccgaatttg ctggcgctgg
     8821 gcgttacctg gctggagtgc attaacggcg gggatcatcc tcggttcctg gtgggcctac
     8881 tgcgaactcg gctggggcgg ctggtggttc tgggatccgg tggaaaatgc ctctttatta
     8941 ccctggcttt ctgccactgc gctgctgcac agtttatccc tgacacgcca gcgggggatt
     9001 tttcgccact ggtcgctgtt actggcgata gttactctga tgctgtcgct gctgggcacc
     9061 ttaattgtcc gttctggcat tctggtttcg gttcatgcgt tcgcgctgga taacgtccgc
     9121 gccgtgccgt tgttcagcct gtttgcactg attagccttg cgtctctggc tctgtatggc
     9181 tggcgagcgc gggacggtgg cccggcggtg cgtttttcgg ggttatcgcg ggaaatgtta
     9241 atcctcgcta cgctgttgct gttttgcgca gtgctactga tcgtgctggt gggaacgctt
     9301 tatccgatga tttacggtct gctgggctgg ggacgcctct ccgttggcgc gccgtatttt
     9361 aaccgcgcga cgttaccgtt tggtctgttg atgctggtgg tgattgtgct ggcgacgttt
     9421 gtctctggca aacgcgtgca gcttccggcg ctggtagctc atgcgggcgt gctgttattt
     9481 gccgctggga tcgtggtttc cagcgtcagc cgtcaggaga tcagcctgaa tttacagccg
     9541 ggtcagcagg tgacgctggc aggatacacc ttccgttttg agcgcctcga tctgcaagcc
     9601 aaaggcaatt acaccagcga aaaagcgata gtggcactgt ttgaccatca gcaacgcatt
     9661 ggtgaactga tgccggagcg gcgtttttac gaagcacgtc gtcagcaaat gatggaaccg
     9721 tcaattcgct ggaacggcat ccatgactgg tatgtggtca tgggtgaaaa aaccggagcg
     9781 gatcgttacg cttttcgctt gtatgtacaa agcggtgtgc gctggatctg ggggggagga
     9841 ttgttgatga ttgcgggcgc attgttaagc ggatggcggg ggaggaagcg cgatgaataa
     9901 aggacttctc acgctactgc tgttatttac ctgttttgcg cgcgctcagg tcgtagacac
     9961 ctggcaattc gccaatccgc aacaacagca acaggcgtta aatattgcca gccagttacg
    10021 ttgtccgcag tgccagaatc aaaacttact ggaatccaac gcgccggtgg cagtcagtat
    10081 gcgccatcag gtttacagca tggtggcgga ggggaagaac gaagtcgaaa tcattggctg
    10141 gatgaccgaa cgctacggag attttgttcg ctataaccca ccgttaacgg gtcagacgct
    10201 ggtgttgtgg gcgctgccag tggtgttgtt actgctgatg gcactgatcc tctggcgagt
    10261 gagggcgaag cgatgaaaga gcccaaaata ccggtgaaaa tgctgacaac actcacgatt
    10321 ttgatggtat ttctctgtgt cggcagttat ctgttaagtc caaaatggca ggcggtacat
    10381 gcggaatatc agcgtcagcg cgatccgcta catcagtttg ccagccagca aaccccggaa
    10441 gcgcagcttc aggcattgca ggataaaatc cgcgctaatc cacaaaacag cgaacaatgg
    10501 gcgttactgg gcgagtacta tctgtggcaa aacgattaca gcaattcgct gctggcgtac
    10561 cgtcaggcgt tgcaactacg tggtgagaac gctgaactgt atgcggcgct ggcgacggtg
    10621 ctttattacc aggccagcca gcatatgacc gcccagactc gcgcaatgat cgacaaagcc
    10681 ctcgcgctgg actctaacga aatcaccgct ctgatgctgc tggcttccga tgcgtttatg
    10741 caggcgaatt acgcgcaagc catcgaacta tggcaaaaag tgatggatct caactcaccc
    10801 cggatcaacc ggacacagct ggtcgagtcg attaatatgg cgaaattgtt gcagcggaga
    10861 tcggattaat catctggtga tgaagattat gttattgagc tgcataaaaa ataatcgaat
    10921 gaacatatgc caaaaataat cactaatcag tattattgca gattaaacaa ataaaaatct
    10981 ttccataaca aatggttatt cattaatcct gacataaact cgcgttatta tgcattaatg
    11041 cagcgaaaag ctctgttgtt aaagggttgc gcaacatacc gcgcaaatga tactgatcat
    11101 aagcgttaaa aaaatctaca aaccaacgca acacaattca tgcctggcag tatgtcacgt
    11161 tctcgcgttt ctgaacgggg aacggcgctc cattgaggaa gttattctga tgaaaaatat
    11221 aaaattcagc ctggcctggc agattctgtt tgctatggtg ctgggcattc tcctgggaag
    11281 ctacctgcac taccatagcg acagccgcga ctggctggtc gtcaatttgc tctctccggc
    11341 gggtgatatc ttcatccatc tgattaaaat gattgttgtg ccgattgtga tctccacgct
    11401 ggtggtgggt atcgcgggtg tgggtgatgc caaacagctc gggcgtattg gcgcgaaaac
    11461 cattatttac ttcgaggtga tcaccaccgt cgccatcatt ttggggatca ctctggcgaa
    11521 cgtcttccag cccggtgccg gggtggatat gtcgcagctg gcgaccgtcg atatctcgaa
    11581 atatcagagc actacggaag cggtacaaag cagttcccac ggcattatgg gcacgatttt
    11641 gtcgctggtg ccgacgaaca ttgtggcgtc gatggcgaaa ggcgaaatgc tgccgatcat
    11701 cttcttctcg gtgctgtttg gtctggggct ttcctccctg cccgcgacgc atcgtgaacc
    11761 gctggtgacc gtgttccgct ccatctctga aaccatgttt aaagtgactc acatggtgat
    11821 gcgttatgca ccggtgggtg tgtttgcgct gattgcggtg acggtggcta actttggttt
    11881 ctcttctctg tggccactgg cgaaactggt gctgctggtg catttcgcca ttctgttctt
    11941 cgcgctggta gtgctgggaa ttgtggcgcg cctgtgcggg ttaagcgtct ggatcctgat
    12001 tcgtattctg aaagatgagc tgattctggc gtactccact gccagctctg aaagcgtact
    12061 gccgcgaatt attgagaaga tggaagccta cggagcaccg gcgtcgatca ccagcttcgt
    12121 ggtgccgacc ggttactctt ttaaccttga tggttcgacg ctgtatcaaa gtattgccgc
    12181 tattttcatc gctcagctgt atggcattga cctgtccatc tggcaggaaa tcattctggt
    12241 gctgacgctg atggtgacct cgaaggggat tgctggcgtg ccgggcgtgt cgtttgtggt
    12301 gttgctggca acgctgggta gcgtaggtat tccgctggaa ggtctggcgt ttattgctgg
    12361 tgttgaccgt atcctcgaca tggcgcgtac tgcgctgaac gtggtgggta atgcgctggc
    12421 ggtgctggtg attgccaagt gggaacacaa atttgaccgc aaaaaggcgc tggcttatga
    12481 gcgtgaagtg ctgggcaaat ttgataaaac tgcggatcaa taattgaaga ttgccggggt
    12541 tatccacccg gcaatgtgtg gatgcctgat gcgacgctgg tgcgtcttat caggcctacg
    12601 ccagacagcg caatagcctg atttagcgtg attttgtagg tcggataagg cgtttacgcc
    12661 gcatccgaca tcaacgcctg atgcgacgct taacgcgtct tatcaggcct accttaaccg
    12721 ttggttaatt tttcaaactc ttcacacccg gtatcaaacc cttccataca gctcaggttc
    12781 aaccagtgca gcgccttttg tttgttcttc tcgataaaac cttcttcacc gtttaagaac
    12841 atcattcccg cccagtactc ggaataaccg gttcgggaaa ttgcagagct gcgtttgaaa
    12901 tactaggttg ccttgtcatc gtcggcctta atacccacgc cgttggcgta aatcaaacca
    12961 agcagcattt gggcatcgac ggcagagtcg ttctccagat cttccgaggc ttttctaaca
    13021 gcgaaatcgc ctttggataa tccggtttac ccgcttgagt atttaccaga atatgcgcca
    13081 gcgttacttc accttcttta ctgcccgctt gcgccgcttt ctctgcaaga acttttgctt
    13141 gtggatagtc cagactgccc ggattggtga ttttgatctg cgccaacagc gcgcaggcat
    13201 cagcatcacc gctgtcggcg gctttctgtg cccaatattc ggctttgctc aaatcgccgg
    13261 agctaaacca gctgtcggca agaaaatatt gtgcgcgtcg gtccccggcc tctgctgcct
    13321 ttaaatactg gctgccaggc tcggagtcgt tggcgtgggc aaagaatgtc aaaaacaaca
    13381 tcaatgcgat aatttttttc attttgataa tacgattggg tagggagtaa ccagtataaa
    13441 gagttcgaat ggataaaaaa acagcctccg aaaggaggct gtagaaagga ctgtattacg
    13501 ccagtgccgc ttcgcgcagg cgagttttca acttgttgta ctcgtcaatc acgtactgct
    13561 cggcggcgcg ctgatcggcg atcggctcga cgcgcacggc gcagtatttg tattccggcg
    13621 ttttcgtaat cgggcttaag ttttcggtaa ccagctcgtt acaggaacca atccaccact
    13681 ggtaggtcat gtaaatcgcc cctttgttcg gacgatcgct gacctgcgca cgggtgataa
    13741 ttttgccttt acgcgagtgc acccaaacca atgcctcatc ttcaataccc agacgtttgg
    13801 cgtcttcggt attgatttgt gcgtagccag gttcatcagc cagcgccgcc agtgccgcac
    13861 agttaccggt catcgaacgg caagagtagt gaccaacttc acgcaccgtt gacagtacca
    13921 tcgggtactc gtcggtgagt ttgtcgattg gcgctaccca gtcgcaggtg aagaactgcg
    13981 ccagaccgtt cggggtatca aacttctctt taaacagata agaagtcccc tgatcggcat
    14041 ctgaagtatc gcggcaaggc cactgaatga agccctgttc gcccattttc tcgtaagtcg
    14101 caccgtagaa atccgggcac agatgacgca actcatccca gatctcctgg gtgttgttgt
    14161 agtgcatcgg ataacccata cgggtggcga tttcactgat gatttgccag tccgttttca
    14221 gatcccattt cggttcaact gctttgaaga aacgctggaa gccacggtca gccgcagtaa
    14281 acacgccttc atgctcgccc cacgacgttg acggcaaaat gacatccgcc gccgacgcgg
    14341 ttttggtcat aaagatgtcc tgaacgatga ccagttccag atcttcaaag gctttacgta
    14401 ctgccgacag ctccgcatca gtttgcagcg gatcttcgcc cataatgtac gcggcacgca
    14461 cttcgccatg cgctgcgcgg tgcggcagct cgctgatgcg atagccggta tgagcaggca
    14521 ggctttccac gccccaggct ttggcgaatt tctcgcggtt agccggatct ttcacgtact
    14581 ggtatcccgg atacgtatcc ggcagcgcgc ccatatcgca ggcaccctgc acgttgttct
    14641 gaccacgaac cgggtttaca cccgcatgcg gcttaccgag gttaccggtc agcatcgcga
    14701 ggctggtcag agaacgcacg gtttccacgc cctggtagaa ctgggttaca cccatgcccc
    14761 acaggatggc ggcgcttttc gcctgggcat acatccgtgc cgcctgacga atctcactgg
    14821 cgctgacgcc ggtgatatct tcaaccgact ccggcgtgta gccttcaacg attttacgat
    14881 actcttcaaa gccttctgta cgggaagcga cgaacgcttt gtcgtacaga ttttcttcaa
    14941 taatgacatg gcccatcgca ttcaacagcg cgatgttcga gccgtttttc agtgcaatgt
    15001 gcatgtcagc aatgcgcgcg gtttcaattt tgcgcggatc gcagacgata attttcgccc
    15061 cgttacgttt agcgttaatt acgtgattcg ccatgattgg gtgggaatcc gccgggttgt
    15121 acccgaaaac gaacactaaa tcggtattat caatttcgtt aatagcattg ctcattgcgc
    15181 cattaccgac cgattggtgc agacctgcaa ccgatgggcc gtgtcagaca cgagcgcagc
    15241 agtcaacgtt attggtacca ataacggcgc gcgcaaattt ttgcattaca tagttggttt
    15301 cgttacccgt accacgcgag gagccggtcg tctggatggc atccggaccg tacttctctt
    15361 tgatggcgct caggcgctcg gcaacgtaat tcagtgcctc atcccaggaa acaggttcga
    15421 gtttgccgcc acgctggcga cggatcatgg gggttttcag gcgcggggtc aggatctggg
    15481 tatcgttaat gaagtcccag ccataataac ccttcagaca cagggtaccc tggttggttt
    15541 tcccctgcgc tgcctccgcc cggacgattt tgccgttatc gacgaccagg ttgattttgc
    15601 aacctgatgc gcaatagggg caaaccgtga cgactttttt catcggtctc gctccagtta
    15661 atcaaatcac gcatacgcgc tctcgactac agtatgcatc ttttatgcca cattttattt
    15721 ggggtcattc cctgatatta cgggcactat ttattcaaaa ctctgacgaa aaacaggctg
    15781 tcgtcagttt tgacgtgacg aaacgaaata ccgcgtgaca gccatcacgc ggcagacatt
    15841 ttattttttc tcgacgacgg gagccgcctg atacccgccg cccagcgatt tcatcaactg
    15901 aatgctgtgg atcacccggc ggctgtccag catcagtaat gacatctctt cggcaagcac
    15961 tggcaaccgg gcttcggtgg cctgtaagcg gctggttaag ccgcgctgat aggcggcctc
    16021 ggcagcgcgc tgggtgaagc gcgttgcttc cacgcgttca gcctgcattt ctcgttcgtc
    16081 gttgagcgtt tgcagacgcg tgccgttgac ggcaacgtca cgcaccgcgt tcagtactga
    16141 ctggttgtaa cgttcaatca tcatgttgct ggcggcgcga gtgccttcga gattggcatt
    16201 caaccgtcca ccgtcaaaca gcggcaattt cagacccggg atgaagttga actggcgact
    16261 ggtttttttg aataaggtat ccaggtggat ggagtccaga ccgaaaaacg ctttgatatc
    16321 aaagctcggg tagaacagtg cccgcgcgga atccacctga tctaatgacg cctgaacata
    16381 ccagcgcatg gcttgcagat ccgggcgtct ggcgagcaac tcataagaga gtgtcgccgg
    16441 aatgccggtc tggactcgtg gtaatgccac cggtttgatc tccggcatat cgctggcccc
    16501 cgcgccaatc aatgcacgca gagattctcg cgtttcggtg atttgccctt tgacggcagc
    16561 aatttgttta tcgaccgcca gaatctgtgc ccgcgcgccg tggaaaggca cttgtgcttc
    16621 cagaccgtgc gccactttac tctggtgcgc tttcaccgcg taatcaatca tatcgcgagt
    16681 ttgttctaac agatcgagca tctgatagct ggcctgcata ctgtaataaa gctgcgctac
    16741 gcccgtggtc agcgatagct ctactgctgc ggtttctgcc agcgcggcat tatgcgcgcc
    16801 aatggcggcg gcaaccgctg agcgatgcac accccacaaa tcaagatcca gtccggcaaa
    16861 caaacctact gtggcttccg tatagtacgg cccgtccata cccagtgagg gcgcatccat
    16921 cgcataaggg cttaaaaagc cgttcgccga gacgcgttgg cggttgagca tccctaacgc
    16981 tgcgacctgt aattgtgaac cggcatctaa caaatcggcc tgggactgcg ctttttcttc
    17041 ccgcagtttc gcttcggcga gggtgtgtga accacttagc gtccgttgga tcagcgcatc
    17101 cagctgcggg tcattgagtt gtttccacca ctgcgcctgc ggccagccgg agctggcaag
    17161 atgaatatcg tcggccagtt tgatttgttc cggtttgagc tgttgatgag gtgcagaatc
    17221 cttacgtacc agggcacagc cggaaatcag cgtcgtgctg ccgagaatgc tgcacaacag
    17281 cagacgtgaa agttgacgat tgatcattgc gtggctcctt gtgcctgttc cgtggcgggc
    17341 gttaatgccg gggctgtcca gtctggcaag cgggcgaaaa gctggcagac gtgttgctcc
    17401 tgttttaata aggtgggcag gatggcctgc gaggcgggtg tctcttcaag cgttatttga
    17461 ggagaacagc tgagtgcggt tgccagaccg gcagcgtatt tttccagggc gtcggcaaac
    17521 tgacctgctc tgtccggctg tagtgcgtta tccagcgcct gcgccgagtt ccaggtggca
    17581 tcccaggcgt gaagaagatc gcggccctga cgaataaccg tttgcgaacg ttcaatcagt
    17641 aaggcgcgtt cttcgctgtc cagttgacgc tccagcgcca cgcgttggca catctcttca
    17701 caggcattaa acgccgcatg cagacctata cgaatttgca gataagtgcg cagagccgtg
    17761 acttcctgct ggcgtgggat ccgcattact ttacttagca tacccagcgc accggctagt
    17821 ttttgcggca gagtgcgcgc ttcactttca ggccagacaa aggtgtaaat caccgcggac
    17881 accacggtac caatgatgat acccagggcg cgatcgcgaa tttccaccag gtcgtacact
    17941 gggccaaaaa cgttttcgag cgtggcgagc gcgaaggtga cgaccatctg tgtgccgata
    18001 taagaagagc gttcagagct ggtggcaatc catgcgccca acaggaaaat cggtgccagc
    18061 acaaacagca attcgacaat attgtccagc cagggcatga ccagtagcgt gaataacagc
    18121 gccagaatcg cgccgcaaaa ggccccgcca aaacgcagca ccatcttctg gtacgacgaa
    18181 ccgacatttg gattagcgac gatcacgcag gtcagcatac aggtgtgaat gccttcccag
    18241 tccacgccgc tgtaaaaggt gtaacagatc aaacaggcga gcaatgtttt taccgcgtag
    18301 cgtatatagt ctggattggt aaaagcatcg gcgaccattg atggcggttt ggcggcgggc
    18361 gttggcggcg tattcgggtt catctggccc agttgtaaca acgtctggca gatattctcc
    18421 aggttacatt cccgtgccgc caccgcttca ctttcactga tccgccagtc gctttgccag
    18481 cactgacctt cagcaacggc atgctgcagc ttgttgattt ctgaggctaa tttttgccga
    18541 aattcaataa ttgcctgaga atcagcaaaa gaggtgggat cgtagcgatt cagcgtcgag
    18601 taaatgtagg ttaccgttgc cacgcagctt tgccaccatg cgctttgagt tcgccagttg
    18661 gcatcgtccg cgagacaaaa gacattcagt ttttgcagcg ccagcgcctc tctttcaatc
    18721 cgcgtttcgg gtagcggtgc gaggctgtct gtcaggtgac taatggcatc atcaagccga
    18781 tcattaagcg cctgatgcat ttgcgaaatg gcacgactgg gaaaccacag cacgccgatt
    18841 aacgtcatca gcaaggttgg atagaggcca acaacgatac accacagcgt taagcgcacg
    18901 accacttccg gatagtcgag catggcgggg aaggtttgcc cgtaaatggc gacaatggcg
    18961 acagcgaaaa agaccagtcc cagccgatgg gtgcgcatca aaaacatgca gcccatcagg
    19021 atcggcccgg cgatgatcaa tcggatcaac ggttcgccgt atgaccattt gtagatcaaa
    19081 aacaggctgc cgatctccag caccgtggca accacaaaca agatcgccac aaatttggtg
    19141 taaaacgcgt tcgactgaat accgtaaaac agcactgcca gcgataacgc cacaaaaggg
    19201 atctcaaaag tcatcgagat cagaatcacc agcaggcagc ctacccagag ttgcacgatc
    19261 tgcggcacgc ggccgggtcg ccgctcgctt aactcttcat gaaagaacgc cagcagcctg
    19321 accaccggta atggcaggga gttgagcgcg ctcattattg cggctcaaga ttagcgactg
    19381 ccgaagcgcc gatgcggaac atttccgggt caggtttatc gaccatgatt ttgaccggaa
    19441 aacgctgggc aacgcggacc cagttaatag agcgagaaac tttcggcagg ccgcccagca
    19501 ccaggccgcc gtcattcggt agcacaccgt agccaatcga atccacttta ccctcgaagg
    19561 ttttgccgct gtcactcatc aggcgaatcg ttgcgggtgt acctgagcga atatttttca
    19621 gatcggtttc gcggaagttg gcgatcacgt accagtgacg agtgtcgatt agggtaaaaa
    19681 tagggcgcat ggcagaagca aattgcccga cggaggtttt gagggaaatg acccggccat
    19741 caaacggcgc gcgaacggtc gccatttcca gatgcagttt ggtcagggca atatccgctt
    19801 cgaccgccgc acgctgggca actaatacat ccacgccgct gacggcgctg gcggctgact
    19861 gcgcctgtaa caatacggca ttaagatccg cttctgcggc gcgctgcgcc gttcttgcac
    19921 ggtcaacatc ttccgctgag acaaaacctt ctttcagtaa tggctcggtg cggcgtaatg
    19981 tatctgtggc ctgtttcgcg gcggcacgtg ctttttctac cgtggcatta accgagtcgg
    20041 caccaaactg ttgggcgtca acgctacgct gggtgagcat aatttgctta tccagcgccg
    20101 cgagggaggc ttcagctttc gccagattgg cttcgtacgg gcgcgggtca atgcggaaca
    20161 gcaaatcgcc ctgtttgact gcctggttgt cggtgaccgc cagttctaca atgcggccgc
    20221 tgacttccgg caccacatca atggtatctg ctgacgcgta agcgtcatta gttgatggcg
    20281 cactgtctac gcgccagata acgaaaacaa gggcaaccag cgccaacgca accactaaca
    20341 gagcagggaa tttactgcga ggagcttttt tcggcgtact ttccatgagc gcgtctcaaa
    20401 tagattagaa aaatgccagc cagaacagca tggcgtagac agcaaaaagg gcggtataaa
    20461 taattccgac aaatgccaga ttgatatttg cccgctgaat aattcgcctc gtgattagcg
    20521 tcaaaataag gctggcaatg gcgcagaaaa accagctggg ataataagcg ccgatcaccg
    20581 ggattgccgg agataatgag catcccgaaa ccatgaagac gggaattatc caggctgttt
    20641 ttttgagttg catcgccatg cgagagagaa ctgttggcat attaaataca tcctgtttgc
    20701 tgcgaacaat atctgtagtg aatatcaata tccattgaag gcgtcagtat ggctattttc
    20761 ttgatgaata aaaatagctt tattcagcca ccgcattcat aacttatata ttttatgtgt
    20821 taattgattg tatgtatttt tataataaat gcttttgggg gcttaagcgc ccccaaaagt
    20881 gagtggcatt aatttggggt tacgatattc acccacaaat cgaaattatc cagacaggca
    20941 ataatttctt ccagtttcgc gccattgcca ataattttgg ctttcttcgc ttttaccaga
    21001 tccgccattt tggcttgtcc tgtcagcacg gcgtgcagat cttcacggct gatgtagaaa
    21061 gaggcgtcgg cttgcggttg cagtgtttta cggtagttaa gcacgctatc gttcagcgtc
    21121 aggttgaggt tatcgccgtt gctcatattg aagttcaggc tgatattttt acccgcagct
    21181 ttcgcgctat cgaggcgaac ggacataaag tcgaacagca tttcgaccga catcccgcga
    21241 atggtgtccg gggaaccggt ggtgccgtgg ctgaacttat gcaccccttc gcgcagctct
    21301 ttcgcgccgg tcaggtagaa accgcgccag gtggcggact cggcctgata gcccagctgt
    21361 tcaaagttat tcgcttgcag attcttcgcg acctggtcac ccgggttggc ggcaatcacc
    21421 tgtttcagca gttctgccga ccagcggtaa tcaccttgct tgttcgcttc ttgcgccagg
    21481 ctgatgacac gggcagaacc gcccagcgcc tgcacgtaac gtttacccat ctccacctga
    21541 ccatacggat gcaggttagc cgggttaccg tcgtaatagc caagatagaa gttatacacc
    21601 gcgcgggcgt tgtggctgac agaaccgtaa tagccacggc tggcccagtt attggcaagt
    21661 gcaggcggca gtttaatcat gtcgccgatt tcattcatgg tgtagccctg gttcgccagg
    21721 tgcagggtct ggtcgtgaat gtacttgata gtatcgcgat atttaccaat ataatcattg
    21781 atatgcttat tgccccggac cggccaggtg tgcggcataa acaggacttc cgcgtcgtta
    21841 ccccacatat ccagcgtttc gttcagatac tcggtccact tgctggtgtc gcgggttttc
    21901 gcgccgcgca gagtgtagaa gttgtgcagg gtatgcgtgg cgttctcggc ggtacacagc
    21961 gctttcaggg ccggaatata gaagtgcatt tcggctggtg cttcgctacc tggggtcatc
    22021 aggaagtcaa actccaggcc gtcgataatc atcttctcac cggttttggt gatggttttg
    22081 ttcggtgcga tgatgctcgg gtagcccgat gccagcgtta cgccaagacc attgccgatg
    22141 ttaccctgag ggttatgcgc cagcagcagg ccgtaggagt aaagtgcacg gtggctcata
    22201 atattacccg ccagtacgtt ttcgctgatg gcttcgtcca taaagccagc aggggcgata
    22261 acctgcactt taccggattt cacatcagct tcagagataa tgcctttcac gccgccatag
    22321 tggtcggtat ggctgtgggt ataaataacc gcaacaatcg gtttttgcgg gcgattttgg
    22381 aagtaaaggt caagggcggc ttttgcgctc ggtggcgtca ccagcgggtc gatgacaata
    22441 atcccggtat caccttcgat aaaggtgatg ttggagatat cctgaccgcg aacctggtac
    22501 attctgtcgg tgactttaaa caggccggaa ataccgttta actgcgactg acgccacagg
    22561 ctagggttaa cggtttcggg cgcgggcgca ttgatatcaa atttataatc ttccccgcga
    22621 taatatggtt tgccattagc atcgcgcaga ataccttcat ccagcagcgg agcgataaat
    22681 ccacgctgag cgtcgtcaaa atcctgacga tcgctaaacg gtaatgattt ggcgtaattc
    22741 tgattaacct gttgggtata ttgagtggcg gctttcgctt cctctttcgc gttagcggta
    22801 tttaccatca tgccgctggc ggcagttaac gcaataacaa ttctgctcaa acggaataac
    22861 cgagagttat tcatataaat ctccatttat catttatgtc gtaaatatgt aatccataca
    22921 aaatacgtca tttaaagctg gggttatcat aattgggaat ttgcgacttt aataagtgga
    22981 agtgtgagct gaacgatcca ttttattagg catttgtgct gaagagagaa aattacgcga
    23041 atttattatc tctgtaaata atatgaggtg ttttatatat ttattatata tattgtactt
    23101 cggcaaatac tgagcacaat ttatcaatgc aaccggtgtt tctttttaaa aaacgtaccg
    23161 gagcaatggg cggtcatgtc gctggcaccg caggattctg cgttgctgtg ccagcctttt
    23221 attcatggtc aatgagtgag gtgattgata aagacaccag gtcgtcagag cgcactgatt
    23281 atccgcatta tgccagtgaa gttgcactgg cttttggttc acgagtggtt ggcctcgaac
    23341 tggcaaaaat gattgtggat gcgtggctgg gcgcacagta cgaaggcggt cgtcatcaac
    23401 aacgcgtgga ggcgattacg gcaatagagc agcggagaaa ttgagattca tccactactt
    23461 gcatggatga gtaatgatta atgtggatag agtttctttt tgaggttggc taatgaaacg
    23521 ctttccgcta tttcttttat tcaccctgct cacgctgtcc accgttccgg cccaggccga
    23581 tattatcgac gacaccattg gcaatattca gcaggcgatt aacgacgctt ataaccctga
    23641 ccgtgggcga gactatgaag attcgcgcga cgacggctgg cagcgtgaag tgagcgacga
    23701 tcggcgcaga caatatgacg accgccgccg ccagtttgaa gatcgccgca ggcagctgga
    23761 cgatcgccag cgccagcttg atcaggagcg tcggcagcta gaagatgaag agcggagaat
    23821 ggaagatgag tatgggcgat gaggttgggt atggagcagg catcgcccct caccccaatg
    23881 gggagaggga gaaaaccagc gcaatattca atatcgagca caatcggtcc cctcgcccct
    23941 ctggggagag ggttagggtg agggggccag cgcgcacatt ccctctcatc acgccacccc
    24001 aatctccatc ccatcaaatc ccgcctcaaa ccccgacggc agtgcgtttt ccatcagcca
    24061 cgcatcaaac tggtggctga tatgggtcag aatcacccgt ggcgagcgga taacctgatt
    24121 cagcgccagc acggtgttta aatcacagtg actacgtggc gcatcctcgc gcggcgggtg
    24181 gctgcaatca atcaccatta cctgcggctg attattgcgt aaaaatttca gcgttttttc
    24241 cggcaagcct gcggtgtcag acagccacgc cacccggctg tgtgccgttt ccagcagata
    24301 accgaaggtc agttttgagt ggttgagcgg caggggcgtg acctgtaacc cctgcaaatc
    24361 aaacaccaca aacggttcca ccgtgtggct gaaatcaagc aggcccggat gtttaaacag
    24421 atcgtcgcag ccctgttcat ccggcgggcc gtaaaccggg atcggatcgc ccacgcccca
    24481 gcgcagcggg aatagcccct gcacgtgatc catatggtag tgcgtcagca aaaactgctg
    24541 gaacgatccg ggcggccagc gatcggcgag atcgtgcagc ccggcgtcga tcagggtgat
    24601 tgcgtcgtta aacttcacta cgccgctgca tggctggcgg cgatactgcg gtgagcgccg
    24661 cgctctggcg caggctacgc actcgcagcc ccatgccgga acgccctgtg cgccgccggt
    24721 gccggtgagc gtgagggtca ggctcatgtt acagcgcctt ggtgaagcgg aagtggctct
    24781 gctcgtagcc ttcacgcagg taaaaacggt gcgcgtcgtg gcgcttcacg ttggtggaaa
    24841 gttcggtcat ttcggccccg gcctggcggg cttcttcttc tgcccacgcc agtaacttac
    24901 tgccgacgtt cagaccgcac gcctgcggca tcaccaccag ttcctgaatt tcgccgatcc
    24961 agttgacatg atgcagatga aactgcaaat gcaggccgat catgccgaca acttcgccat
    25021 ccagcagcgc cagatggtag cgcatgctcg ggtagcgcat gctcgggtcg cgcaggttgg
    25081 cgttaaagcc cacgcgaaac gcgtggtggt caaactccgc ctgttttagc tcacaaatca
    25141 gcgcgtaaac cgcgtcggta tcgtactgcg tggccgggcg aagctcacaa gcaggcatgg
    25201 tgtttctcct tctgatgaat cagcgtcagc agtgtgtcga ccgactggcg caggctgccg
    25261 tcattgttga gcgtatggca atcctgtgga gtatagcggg cggcgcgcgc cagccgggcg
    25321 ttaatttcac tggtgttttc acggccacgg tttttcaggc gctggcgcag gatctccggc
    25381 gaaacctgta aacagacggg cagcagcgcc gattgatagc gcgcccgtgc ctgcggcaaa
    25441 tgcgcgcgtg aaccgttgac gactacgtcg aatccagcgt gcagccagag gtcaatctcg
    25501 atgccgacgc cataatacag accgttagcg tgccagctta aggccagcag gttttgcccc
    25561 gcgcgggtaa aaaactcccg ctcactcagg gcgatatggt tttcacttcc ggcgctggcg
    25621 tcgcgcgtga tgtagcgatg cgccaccagt aactgcgttt gttcccgcag gcggagttcc
    25681 gccagcaggc tgtctttccc ggagccggac ggccccatta accaaatcag ttttcccatc
    25741 atcagaacac ccttttaccc tgacgccaga cgtggtcgat atgaatgtga ttgccctggc
    25801 gatgcgccag caccaggtca gcgcgtttac cctcgccaat caccccgcga tcctgaagat
    25861 tcagcgcctg cgccggattt ttggtcacca gcctcaccgc ctgcggtagc gtaaagcggt
    25921 tgctctcgtc atcggcgacg cggaacgccg catccagcag gctggcgggg tagtagtcgg
    25981 aagagaggat atccagcaga ccaagctgcg ccagttcact ggccgccaca ttgccggagt
    26041 gcgagccgcc acgcacgata tttggcgcac ccatcagcac gttcatgcca tgcttacgcg
    26101 aggcttccgc cgcttcgaac gtggtggtaa attcggcgat cacgctgcca agttggtggg
    26161 attcggcaac gtgggcgtgc gtggcgtcat cgtggctggc gagcgcaatt tgtcgcgcgc
    26221 ggcacagggc ggcgatggat tcgcgattcg gctgcgacca gcgtgcggcg agcgccaatt
    26281 gctcttcttc gtactgctgc atctgcgcat cggtgaggga gtatttgccc tgataatatt
    26341 cgcgatactt ctcgcggtta gcgaactggc gctggcccgg tgagtggtcc attagcgaca
    26401 ccagcgtcac cggttcgcgc tgaaccagtt tttcaaacag cggcagtgtg gtgtgatgcg
    26461 gcagctcgca gcgcaggtgc agacggtgct cggcgcggtt gacgccgcgt ttctgcgtct
    26521 cttcgatggt gttgatcatc ttctccagat tctccagctg atcgccgccg tcgcgcacgt
    26581 cgccaatcgc caccgcgttc agcacggtgg tgatgccgct tgccaccatc agcgcgtcgt
    26641 ggctgctcat cgccgaatgg gcgggccagt cgactttcgg gcgcggggtg aagaatttat
    26701 cgaggttgtc ggtatgcagc tcaatcaggc cgggtagtag ccagccacct tcgccgtcca
    26761 tcgcttctgg caggcggctc tggctttcgg caaaggcgcg gatttcgcca tcctgcacct
    26821 ccagcgaacc gtgcaccacc tcgttttcca gcaccagctt aacgttattg ataatcatga
    26881 agaggctccc attgggtgca ggcggtcggc gacgtcatta cgtacagctt cgtcatggaa
    26941 gatgccgacg atggctgcgc cacgggcttt ggcttcgtga atcagttcca ccaccgcggc
    27001 gctgtttttg gcgtccagcg aggcggtagg ttcgtcgagc agcagaatgg ggtagtcgac
    27061 gataaagccg cgggcgatgt tgacgcgctg ctgttcgccg ccggagaagg tcgatggtgc
    27121 caggtgccac aggcgttccg gcacgttcag gcgggtgaga agacgcgcgg ctttagcggc
    27181 gcaggcttca cgcggaacgc cggtatcgag cagcggctgc atcaccactt ccagtgctga
    27241 gatacgcggg atgacgcgca gaaactggct cacccagccg actgtggttt tgcggatttc
    27301 caccactttg cgcgctggcg cggtgaccag gtctacccac tcgtcaccgt gtttgatctg
    27361 gatttgacct tcgtcgggta gatagttggc gtacagcgag cgcagcaggg ttgatttgcc
    27421 gctgccggag tgaccgtgga gcaccacgca ttcgcctgcg ttgacggtga gcgaggcacg
    27481 ttgtaagacg ggcaggcgta cgccgttttg ctgatgcagg atgaaggttt tactgacgtt
    27541 ttgtacgtta atcatttttc gcctcgtggt tcgggtgtcg gatgcgacgc tgacgcgtct
    27601 tatccgacct acggggagcg catttgtagg ccggataagg cgtttacgcc gcatccggca
    27661 ccggctcaat tctgcaaaac cgatgacacc agcagttgtg tatacggatg gtgcgggtcg
    27721 tcgagcacgc ggtcggttaa cccactctcc accacttgcc cctgcttcat caccagcaaa
    27781 cggtccgcca gcaggcgggc gacgcctaaa tcgtgggtga caatcactac cgcgaggttc
    27841 agctccacca ccaggccgcg cagcaggtcg agcaggcgtg cctgtaccga cacatccagt
    27901 ccgccggtcg gctcgtccat aaacaccagc ttcggatgcg tcaccaggtt gcgggcgatc
    27961 tgcaaacgtt gctgcatgcc gccggaaaag gtggtcggca ggtcgtcgat ccggttggcg
    28021 ggaatttcca cctcttccag ccacttctgc gcggtggcac gaatatcgcc gtaatgacgt
    28081 gccccggtcg ccatcagccg ctcgccgata ttgccgcctg ccgacacctg gcggcgcagg
    28141 ccgtcgagcg gatgctgatg cactacgccc cattcggtgc gcagcaggcg acggcggtcg
    28201 gcctcgctca ttgcgtacag cgaacggctc tcatagtgaa tctccccctg ctgcggcgtc
    28261 aggcgcgcgg agattgattt cagcagcgtg gtctttccgg agccggactc cccgacaatg
    28321 cccagcactt cccccggcca taaatcaaaa gagacatcgc taaagccttt gcccggcgcg
    28381 taaaggtggg tcaggttatt gaccgaaagt aacggttgat tcattggttt tttgcctcgc
    28441 tctgttggcg gcaataatcg gtatcggagc agacaaacat gcggtttccg gcgtcatcca
    28501 gcaccacttc atcaagatag ctgtgggtcg atccgcagat ggcgcatggc tcatcccact
    28561 gctgaacggt gaacgggtga tcgtcgaaat cgagactttc cacgcgggta aacggcggca
    28621 ccgcatagat gcgcttctcg cgccccgcgc cgaacagttg cagggcgggc atcatgtcca
    28681 tttttgggtt atcgaatttc gggatcggcg acgggtccat cacgtagcgc ccgttcacct
    28741 tcaccggata ggcgtaggta gtggcgatat gaccgaagcg ggcgatatct tcatacagtt
    28801 tcacctgcat cacgccgtac tcttccagcg cgtgcatggt gcgggtttcc gtttcgcgcg
    28861 gctcgataaa gcgcagcggt tcggggatcg gcacctggaa gataatgatc tgatcttcgg
    28921 tcagcggcgt ttcggggatg cgatggcgcg tctggataag cgtcgcatcg tccgtacgtt
    28981 cagtggtgtt taccccggtc acgcgcttaa agaagttgcg aatcgacacg gcgttggtgg
    29041 tgtcgtctgc gccctggtca atcaccttca gcacgtcgct ttcgccaatc acgctggcgg
    29101 tgagctgaat gccgccggtt ccccagccgt agggcatcgg catctcgcgc ccgccaaacg
    29161 gcacctgata accggggatc gccaccgctt ttaagatggc gcggcggatc atgcgtttgg
    29221 tctgctcgtc gaggtaggca aagttgtagc cgctcagatt agccatggtt ctgctcctgt
    29281 tgcagacgtt tgagtagctc cagttcggcc tggaaatcga cgtagtgggg gagtttgagg
    29341 tgcgagacaa agcctgcggc ttcgacgttg tcggcatgtg ccagcacgaa ctcttcatcc
    29401 tgcgccgggc ctgtcgcgtg ctcgccgtac tccggagcct gcaacgcacg gtcaaccagc
    29461 gccatcgctt tgcgctcgct catgccgaac accagcccgt agccgcgcgt gaagtgcggc
    29521 ggctcatccg gcgggtcaat aaaaccgttg accatttcac actcggtcat cagcagttcg
    29581 ccgacgttca ccgcaaatcc cagctcttcc ggcataatcg acacgtcgat gtaaccactg
    29641 cggatttcgc ccgcgaacgg gtgattgcgt ccgtaaccgc gctgggtaga gtaggccagc
    29701 gccagcaaat agccttcgtc gccgcgcatc aactgctgca aacgggaaga acgtgaacag
    29761 gggtaaactg gcggagtgcg ggtgatgtca tccggctgtg cgccgctatc ctcttcaaac
    29821 ttcgccagcc cctgacgcgc cagcaggctg aaaacgtgcg gcgacgggga ttgttcgctg
    29881 tctgcggtgg tcagcgtcgg cgcttcgccg tttgccagca gggtgaaatc gagcaggcga
    29941 tgggtgtagt cgtaggttgg gccaagcaac tgaccgccgg gaatatcttt ataaaccgct
    30001 gaaatacgcc gttcgagacg catctccgtg gtgtcgagcg gctcgcttac cgccagcttc
    30061 gccagcgtgg tgcggtaggc gcgtagcagg aaaatcgctt cgacgttatc gccgctggcc
    30121 tgtttcagcg ccagcgccgc cagttcgcgg tcggcaatgc cgccttcggt catcacgcga
    30181 tctactgcaa gattaagctg ctgttcaatc tgggcgacgc tcagttcagg caaatcggta
    30241 ttgccccggc gtcggctctc ttgcagggcg tgggcggcgt cgatctcctt ctcgccccct
    30301 ttcacggcaa cgtacatcag cacacctcca catgagtggt tcgcgggata gccagcagcc
    30361 gctcgccgca ggtcaggatc aggtcgatgc cgagcgggaa cgggtgcggg cgctcggtga
    30421 gttcgtgcag aatgcactcc ggcagctgcg gagcgatcat tcgttcttcg gcaatacccg
    30481 cgccagtaag gcgcagcatg cgcccgccgc tcaggctgac gacctgtaaa atcagcgtcg
    30541 cgcccgcttc cggcgcaaca gcggtgccgc tggaaagagc gttgagctgt tcgctggaaa
    30601 tcgcctcatc cgttaccgcg aaggtcgcca gttccgactg attgaccagc ggcgcgttgg
    30661 tatgaaaacg caggctctgg ctgacgatat cgttacttaa tggcgcggaa agccacaccg
    30721 gcgtgtcgtt atcagccagc gtcagcagca cgctggtggt ggcgatattc agcggctgcc
    30781 agccgcgttt gagctgatgc agggcgacaa tcacgcccgg ctcgctcatg gcctttaaca
    30841 ggcgacgaaa actgtgctgg gcatcctgca caggaagcat aaaagcggtt tccagggtca
    30901 tgcgttgtct ccgcgaacca tcgtaaagaa gtcgacccgg ctggcgttca cttcggcctg
    30961 gcgtgcggca atgcgtgcca tacggtcagc gtccagtggg gcaataaggg tttctgataa
    31021 gttttgaaaa tggcgagatt gctgcatcaa cgcgtcaatc agcgcgcagc gttcggcgtg
    31081 ctgtttgtcg cgccccagca cccagctgta gccgaggggg ccgtcagtca ggcgtacggc
    31141 ggcgcgggtc agcgtggcgt cgccggcaaa aaagcgttcg ccggtaccgc ccattcgcgg
    31201 ctgaatctgt accaggccgg tttcggcggt gcggatcact tcatagttgg cggtgatgtt
    31261 cagcgcgttc aggcgggctg ccagttcagc cggttggctg tgcgccagca tggacatcca
    31321 gtgctggcgg gtcgcgatat ctgcgtgcat tcagtgctcc atagtgaatt caatcatgtc
    31381 ggcgcgcgtc aggctgacgg agtactccgc cgggctgctt tcaccgtcac ggtggttaag
    31441 ggtgcgcacg cacagcagcg gcgacatatt cgggatttca agacgctggc actctttggc
    31501 ctgggcgcgg cgggcgctga tccgcgtctg gctgcggcgc agcgcaatcc cggtttgctc
    31561 gcgcagaaaa tcgtgcagcg agccgctgtc gaagcgttgc agcgtcggcc aaagggtgag
    31621 gtccgcaaag tagtggtcga ttaaacagag cgcgatgccg ttgacccggc gcagggtgcg
    31681 caggtggatg acgttctccc cctcggttat ccccagcgcg tcagcgacgt gaccagatgc
    31741 cgggcgcagc accgacagca gtttttcgct ggtgggatgg ctgccctgat ccagcaggtt
    31801 ctggctaaaa cgcgcctggg cgttgagcgg gtaatcgaac gggcgcatca gcaccagcac
    31861 gccgacgccc tgacggcgct gtacccagcc tttttccacc agttggtcga tggcgcggcg
    31921 cagggtgtgg cgatttacct caaagcgcgc cgccagctgc tgctcggtgg gaagatagtc
    31981 gccgcagcga tagtgttgac gaagctcctg ctcaagtttt gcggctatct cctgatagcg
    32041 tgttgggtag ctggtcggat gtgtagacaa gtgcatagat atcaatgcct cgcttatcag
    32101 ataaagtgct tacgcaaccg ttgagagagg aaatccagca ggctgaccgt gacgatgata
    32161 agcaccatca gggcgcaggt ttgttggaac tgaaaaccgc gaatcgcttc ccacagggtg
    32221 acgccgatcc cgcctgcacc gaccatgccg acgacggtcg ccgagcggac gttggattcg
    32281 aagcgataga gggagtagga gatcagcagc ggcattacct gtggcagcac gccgtaaagg
    32341 atctcttcca gcttgttggc gccggtggcg cgaatgcctt ctaccgggcc gggttcgatc
    32401 gcttccaccg cttcggaaag cagcttggag agcacgccgg tggtgtggat aaatagcgcc
    32461 agcacgccag cgaacgggcc gagaccgacg gcgaccacga acagcatggc gaagaccatt
    32521 tcgttaatgg cgcggcaggc atccatcagg cggcgaacgg gctggtaaac ccaccacggc
    32581 accaggtttt cggcgctcat cagaccgaag gggatggaga gaaccactgc cagcgcggtg
    32641 ccccagacgg cgatttgcag cgtgacggcc atttcggtga ggtaatcctg ccactggctg
    32701 aaatcgggcg ggaagaagtc ggcaacgaag gttgccatgt tgccgccgtc tttaatcagc
    32761 gtaagcgggg ccatttccgc gccctgccac gagacgacca gcacggcgag aacaacggcc
    32821 cagctcagaa gcgagaacca gctacgcttg ggtggggcga tggtgatggt ttgcatgttt
    32881 ggctccgttt aatgttttct ccctctccct gtgggagagg gccgggatga gggcatcagc
    32941 gtgcgcattt ccccctcacc ctaaccctct ccctcaaggg agaggggacc gatcgagcac
    33001 attactgcac cgctttactc accgaactca tcgcgcttaa cgcgttgttc aggcggtcca
    33061 agtcatccag ctgcgcctga atctcggtgg ttttcgccag cttgtcctgc tcattcagtc
    33121 ctttattgct ttttacgccc tgcatctctt taaacagtgc gagctggcga atcggcacca
    33181 gttgcaggtc gctggaggcg cggaacgggg cccagcccag gcgttccagc accgctttct
    33241 cttccggcgt ttttccgtag ttcataaaga agtcgtagat cttgtctttg gtggtttcgg
    33301 agagattctt gcgccagacg atcggatcgc ctgggatcag cggcgacttc cagatcacct
    33361 tcagttcttt cagcttctct ggcgcggagg ttttcagctt gtcgaggttt tcggtgttgt
    33421 tggtggcaac atccacctgc ttgttagcga cggccagcgc gttggtttca tgcccagcgt
    33481 tgacggtgcg cttaaagtcg ctggcggaga tattgttttt ggcaaagacg tagtaaccgg
    33541 ggacgaggaa gccagaggtg gagttaggat cgccattgcc gaaggtgaga tctttccgct
    33601 tcgccagcag atcgttcagg ttgttgatcg gactatcttt gttgacgatc aacacgctcc
    33661 agtaacctgg cgatccatcc gccgcgaccg tctgggcgaa gacctggccg ttggcgcgat
    33721 ccaccgcttc cattgctgac aggttgccgt accaggcgat atccacttta ttgaagcgca
    33781 ttccctggat aatgcctgcg tagtctgggg caaagaaggc gttcaccttc acgcccagct
    33841 tcttctccat atcctgtaag aatggcgtcc attgcggttt caggttttgc tgtgattccg
    33901 ttgaaataat gccgaaattc agcgcctttt cctgctcttc ggcgtgtgcc gggcttaaca
    33961 gggtgctgag gctgaacatg ctggtgaagg ccagcgaggc aattatctta gcgttcattc
    34021 gttttcctca atgatgggga tgtcaggcag ctttcgcgtt ctcttcgatg cgattaatgc
    34081 tgcggtagag atggtcaaaa cgttcgttat caaactgttg gctgctgccg tcgtagaaaa
    34141 cgtgcccctg gcgcagggcg acgatgcgtt cgcagtagcg cagggcgtaa tccacctgat
    34201 gcagcgtgac gactacggtg atgccgtcgt tctgattgat gtcgcgcagg gtgtccatca
    34261 cgatgcgggc ggattccggg tccagcgagg cgatgggttc atcggccaga atcaccttcg
    34321 cctgctgcat cagcgcgcgg gcaatcgcca cacgctgctg ctgtccgccg gagagggtgg
    34381 aaacgcgttg atgggcaaaa tgcaccatgc caacgcgggt cagcgcctgt aacgcgcgtt
    34441 gtttctgctc gcgggtgaac cagctaaaac aggtgcgcca gaacggcgtg ctgccgagcg
    34501 cgccaatcag cacgttctcc agtacgctca ggcgattcac caggttgaat tgttggaaga
    34561 tgtagccggt atgggcgcgg cttttgcgga tatcgcgcgc cagacggcct tcgcgctgga
    34621 ctgtgcggcc cagcagctcg atatggctgc cggcggattt atcgccggta atcaaaccgc
    34681 ttaagtgacg taaaagtgtg gatttgccgg aacccgacgg cccaagcaga gccaccattt
    34741 cactgtgatg aatgttcaga tcaaccgcat gcagcgcttg atgctgattg aaggttttgg
    34801 cgagcttctc gacacggata atcgtttgca tgatgcagcc tccctaaaaa agtggctcca
    34861 tcgtggcgga ttattgtgac gatttggtta attaaaagtg actaacagat gaagagttaa
    34921 cgggaattcg atgacagtgc gggagggcgg gcctcccggc gggttattgc gttggttgtt
    34981 gtttgacgac attaatcatc cacggtacgc caaatttatc ggtgactttg ccgaagccat
    35041 gcgcccagaa agtttcctgc caggccattt caatttttcc gttagcggca aggttatcaa
    35101 accagcgttt tccttcttcg acctgttgcg aatcgagcac cagcgtaaag ccggagtagc
    35161 tggcttttcc tgacggtata gcatcgctca tcatgatgtc gcttccggca atgcgcacgt
    35221 tggcgtgggc gatggcggta tcgggaaatt gcattccgga agggcagttc tcggcgctgt
    35281 cctgcgctga ttttggcatt tcgccgaagc tgattttata gagcagttcc gcgcccaacg
    35341 tacgttgata ataggcaatc gcgtcggaac agttaccggc aaaagagagg tagggactta
    35401 acggcatgat ggtgacctca gttaagagaa gccagttaag tgtagttcga aatttataca
    35461 gatgagaggg aagcctgata aggcgcggtc agcgtcgcat caggcagtcg gcatcgttgc
    35521 cggatgtggc gcgagcgcct tatccggctt accgatcaca atacaatcag ttctttttca
    35581 caaactcaga tttcagtttc atcggaccaa aaccgtcgat tttgcaatcg atgttatggt
    35641 cgccttcaac caggcggatg tttttcactt tggtgccaat tttcagcatc gaagagctac
    35701 ctttcacctt cagatcttta atgatggtaa cgctgtcgcc gtcagccagc agattgccgt
    35761 tagcatcttt aacgatcagc tcgtcgcttt cctgtgcagg ttctgtgtcg ttccattcgt
    35821 aggcacattc cgggcagatg tacatgccgt tatcttcgta agtgtattcg gagttgcatt
    35881 ttgggcagtg tggtaatgac atgtggattt cctcaaaagt cagcagggca aaagcgccaa
    35941 aaaacggcag attgccgaaa aaggccgcaa ttatacacaa aatccctgag tttgtcgggg
    36001 gtactgctga ttttagacga cgatcgtttt catttgaaag gaaacgcaaa aagaaaataa
    36061 tctttttgcc tctatttatt atttaagata atgttaatgc tctacctgtt ctttttttat
    36121 tcacatgata tttttatctt tctgacgttt taaacgcatc agaagacgtt atttattctg
    36181 ataaaaaaca acacactaaa tataaaat
//
LOCUS       DADLFA010000019        34375 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000019 DADLFA010000000
VERSION     DADLFA010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34375
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.019"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..655
                     /locus_tag="JQH65_000816"
     CDS             <1..655
                     /locus_tag="JQH65_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005113789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HAY6138312.1"
                     /translation="ASWQRLQTSMQNAMNIFFASGFGNELTSAFDAISAAVNGSGGAF
                     EMFGHIAGKIVEGATDAFTELHDTVILTFRIIEYYASKMGITGEQLKAWGEMAAYAAG
                     VAMFAGSIWKLGVAFKWLLTMLNPLTKLLGVMKGIAAIGGIEAATTGGSGGEGKDKPN
                     KPGRGPKVGKVGGAAALSGGPVGWSIAVGSVGGDYAWDKVMTLTTPCMDRTPPPGAI"
     gene            619..912
                     /locus_tag="JQH65_000817"
     CDS             619..912
                     /locus_tag="JQH65_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6138313.1"
                     /translation="MYGQDTTAGGNLKKDSPLGKLWDFLHSPDLPTNPDFAKPADKTG
                     WVSSYSNPTYVPPTPTITAPQLKVDMAGNPLMTPPAWLNQPLNVNVNVKVQDG"
     regulatory      2493..2589
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2624..3412
                     /gene="thiM"
                     /locus_tag="JQH65_000818"
     CDS             2624..3412
                     /gene="thiM"
                     /locus_tag="JQH65_000818"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HAY6138314.1"
                     /translation="MQVDLQSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQLRAQSMCAAVEQAKSSQTPWTLDPVA
                     VGALDYRRRFCLELLPHKPTAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEMDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            3409..4209
                     /gene="thiD"
                     /locus_tag="JQH65_000819"
     CDS             3409..4209
                     /gene="thiD"
                     /locus_tag="JQH65_000819"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAY6138315.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIESDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQYV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLCTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            4274..5093
                     /locus_tag="JQH65_000820"
                     /pseudo
     CDS             4274..5093
                     /locus_tag="JQH65_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 25 family protein"
     gene            5145..5891
                     /locus_tag="JQH65_000821"
     CDS             5145..5891
                     /locus_tag="JQH65_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY6138316.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(5865..6830)
                     /locus_tag="JQH65_000822"
     CDS             complement(5865..6830)
                     /locus_tag="JQH65_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAY6138317.1"
                     /translation="MSGARLHTLLPELTTRQSVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(6827..7831)
                     /locus_tag="JQH65_000823"
     CDS             complement(6827..7831)
                     /locus_tag="JQH65_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HAY6138318.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLERKGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSITRHAQQKRTTTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGINAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            complement(7828..9105)
                     /locus_tag="JQH65_000824"
     CDS             complement(7828..9105)
                     /locus_tag="JQH65_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY6138319.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPGTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVIAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFCESDNEITAIKVDDRDIALTQGEVK"
     gene            9362..10414
                     /gene="fbaB"
                     /locus_tag="JQH65_000825"
     CDS             9362..10414
                     /gene="fbaB"
                     /locus_tag="JQH65_000825"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HAY6138320.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVVSTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            10722..11576
                     /gene="gatY"
                     /locus_tag="JQH65_000826"
     CDS             10722..11576
                     /gene="gatY"
                     /locus_tag="JQH65_000826"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HAY6138321.1"
                     /translation="MHVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATEPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            11605..12807
                     /gene="gatZ"
                     /locus_tag="JQH65_000827"
     CDS             11605..12807
                     /gene="gatZ"
                     /locus_tag="JQH65_000827"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HAY6138322.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKEYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIVRGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQPLAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPH"
     gene            12877..13329
                     /gene="gatA"
                     /locus_tag="JQH65_000828"
     CDS             12877..13329
                     /gene="gatA"
                     /locus_tag="JQH65_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HAY6138323.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVEVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            13360..13644
                     /gene="gatB"
                     /locus_tag="JQH65_000829"
     CDS             13360..13644
                     /gene="gatB"
                     /locus_tag="JQH65_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HAY6138324.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            13648..15003
                     /locus_tag="JQH65_000830"
     CDS             13648..15003
                     /locus_tag="JQH65_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAY6138325.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSVSLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFITWRRARGFIKQEKAVLA
                     E"
     gene            15051..16091
                     /gene="gatD"
                     /locus_tag="JQH65_000831"
     CDS             15051..16091
                     /gene="gatD"
                     /locus_tag="JQH65_000831"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAY6138326.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGSMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            16191..16970
                     /locus_tag="JQH65_000832"
     CDS             16191..16970
                     /locus_tag="JQH65_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY6138327.1"
                     /translation="MTMNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRF
                     LEQKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMT
                     VILDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMH
                     GSIAERSLQDINADLMFVGADGIDAVNGITTFNEGYSVSGAMVTAANKVIAVLDSSKF
                     NRRGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(17052..17950)
                     /gene="yegS"
                     /locus_tag="JQH65_000833"
                     /pseudo
     CDS             complement(17052..17950)
                     /gene="yegS"
                     /locus_tag="JQH65_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
     gene            18364..18681
                     /locus_tag="JQH65_000834"
     CDS             18364..18681
                     /locus_tag="JQH65_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6138328.1"
                     /translation="MKKIAAISLISIFIMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARSRISGAENIVKNDATHFCVTQGKKMQIVELKTEGVGLHGVARLTFK
                     CGE"
     gene            complement(19011..20372)
                     /locus_tag="JQH65_000835"
     CDS             complement(19011..20372)
                     /locus_tag="JQH65_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HAY6138329.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREYFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGVAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(20693..22093)
                     /locus_tag="JQH65_000836"
     CDS             complement(20693..22093)
                     /locus_tag="JQH65_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000911981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HAY6138330.1"
                     /translation="MLKGLRTLFSRRVLFNVEMNNSVDCELLLIQQQLQALIAEPGNY
                     LAHDCDLNDTLVLCQLTHHHNGYIREKAVQYLGHKEDVAAIEELLLAANDWVKEVRLA
                     ATIALRHLMKDKNAAAFAVNLPMIRNLLQCQRYDHRAFVDEILRYLLTENNRPQLTYW
                     LTCPDKKLSRAVLQTLIEYGYFNDESSLLSILDKPDEGLRMLATTHWLRQQLPLSEAV
                     LTRLLKDRWPRIRQATLFSLTDRAIEMPPALHSTLLLDNNMLIRLRAKNMLHEVMDVP
                     QFWRHVVTSAEYTPSQRRAALYGLDSIHDPNILKLAEWGLSQNVFPLRLAAMHILAKA
                     NPRCGVKETILTTLANPDAAGLRFMVNICVWCRVPLTFEEIRQLQENAPSVKHACAYC
                     RLYHNLNKWDGLILLLQSQHKLTEEFAGKQLAIWQRNFNLSGIQPNALQRQQLQALFT
                     RNPELHNRLWGYIPFK"
     gene            complement(22216..22548)
                     /locus_tag="JQH65_000837"
     CDS             complement(22216..22548)
                     /locus_tag="JQH65_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HAY6138331.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            complement(22739..23461)
                     /gene="baeR"
                     /locus_tag="JQH65_000838"
     CDS             complement(22739..23461)
                     /gene="baeR"
                     /locus_tag="JQH65_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HAY6138332.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            complement(23458..24861)
                     /gene="baeS"
                     /locus_tag="JQH65_000839"
     CDS             complement(23458..24861)
                     /gene="baeS"
                     /locus_tag="JQH65_000839"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HAY6138333.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            complement(24858..26274)
                     /locus_tag="JQH65_000840"
                     /pseudo
     CDS             complement(24858..26274)
                     /locus_tag="JQH65_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(26275..29352)
                     /gene="mdtC"
                     /locus_tag="JQH65_000841"
     CDS             complement(26275..29352)
                     /gene="mdtC"
                     /locus_tag="JQH65_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HAY6138334.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGKLYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            complement(29353..32475)
                     /gene="mdtB"
                     /locus_tag="JQH65_000842"
     CDS             complement(29353..32475)
                     /gene="mdtB"
                     /locus_tag="JQH65_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HAY6138335.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLIFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYSAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            complement(32475..33722)
                     /gene="mdtA"
                     /locus_tag="JQH65_000843"
     CDS             complement(32475..33722)
                     /gene="mdtA"
                     /locus_tag="JQH65_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HAY6138336.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGMIKVKARFNNQNDVLFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            34001..34057
                     /locus_tag="JQH65_000844"
     CDS             34001..34057
                     /locus_tag="JQH65_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HAY6138337.1"
                     /translation="MMKLLIIVVLLVISWPAY"
BASE COUNT         8527 a         8640 c         8988 g         8220 t
ORIGIN      
        1 ggcaagctgg cagcgcctgc aaaccagtat gcagaacgcg atgaacatct tctttgcttc
       61 tggcttcggc aacgaattaa ccagcgcttt cgacgctatc agcgccgctg ttaacggctc
      121 tggtggggcc tttgagatgt tcgggcatat tgccgggaaa atcgttgagg gtgccacaga
      181 tgcgtttaca gagttgcatg acactgtgat cctgaccttc cgcattattg agtattacgc
      241 cagcaagatg gggatcactg gtgagcagct taaagcctgg ggcgaaatgg cagcatacgc
      301 cgccggggtg gctatgttcg ccgggtctat ctggaagttg ggcgtagcct ttaaatggtt
      361 gttgaccatg cttaacccac taaccaaatt actgggggtc atgaaaggga tcgccgctat
      421 tggtggcatt gaagccgcta ctactggcgg atctggtggg gaaggtaaag acaagccaaa
      481 caaaccgggt cggggtccga aagttggcaa ggtaggcggc gcggcggctc tctctggcgg
      541 tcctgttggc tggagcattg cggtcggttc ggtaggtggg gattatgcct gggataaggt
      601 catgacgcta acgacgccat gtatggacag gacaccaccg ccgggggcaa tctgaaaaaa
      661 gatagcccgc tgggtaaact gtgggatttc ctccatagtc cagaccttcc gactaatcca
      721 gactttgcca aaccagcaga taaaacaggg tgggtaagct cttactccaa tccaacttat
      781 gtacctccta cgccgacgat tacagcgcca cagcttaaag ttgatatggc aggtaatcca
      841 ctgatgacgc cgccagcgtg gctaaaccag ccgctaaacg tgaacgttaa tgtgaaagtg
      901 caggatggat aggttaagga tctggtacgc tctgaagttg agagcaataa tcagcaggct
      961 ttcaacatgc taatgcaggg cgggccgaac taaccacgtt tgacaccccc aaccacaggc
     1021 ccccggctaa cccccggcgg gcttttttgt tatctgtaac catgaaatca acactttgct
     1081 aaccccctaa ataaatggct ttaaatccgt ttaaatatgt ggtaaagggc gatcaaatgg
     1141 attgtatacc cggtaaaaga atcagtttta gctcaaaaag taatcaattg tgtaactagt
     1201 gggactcccc cagcgcttct aactatttgt ttttatggtc aattaattat cattacaaag
     1261 tgcaagtgtg tagcatttta catgcttaac cccaaaatta tttttctggg gaaaacttcg
     1321 aacacaaatg agatcgatta tcacttgaga attggttctc aatccctctt tcgagtgcga
     1381 ctcattccca tctacagggg cggtagccac cgccctaaac cctttttgtg tttttgccac
     1441 aagaaagggc ctgaagaggc ggacgcctca aaaggggagt ttatggggtt agtgcgtgga
     1501 agttatccac aggaaaaggg cgttactcac aggcagatca gttttacagt ttactctgtg
     1561 gaatggctcc cggatgggta cagaagagcc acagttatgg ggcgtacaca gcgcccagga
     1621 ggcggaccta ttgaaagggt acaggtctaa cctgttgcgc atatgttaaa tagttgtcta
     1681 aaagttgacc aaatcggacc tttcaccact ttcatttatg caatattcac acggacgctc
     1741 tgtaacgctc tgtaagcctc gcaatgggtt agctatggga ttgtatagaa agaggagaga
     1801 agacgccaca gcggcgcgta aagagagcca cagcaggccc ctcgcctgga tatttgggaa
     1861 taataaacat tctcatctat gggtctggtt accctgcctg gctactgtat ccaaactccc
     1921 agatattttc ccacccaact ggaaggaatc cgcccctatt ttatggtcgt gtataggtta
     1981 ggctgaggga ggctcgccaa tgtacacccc cctggatttg gtgatctgtt aagttattga
     2041 ttttgatcga ttttagatca atcatccgtt gccgggatta cccccaccca gacggatggc
     2101 acacaatgaa taacgataaa gtaaaccacc ctaaacactt ccaatacttg ggccgcatgg
     2161 tcactgttta ccctacatac atcattattg acggtatcaa gatcagccgt tcccgtcttt
     2221 cctttgcatt ccaattggaa ttggcaaaag cacttcgact gcatgaagaa agcgtatgca
     2281 gaaaatagat ttcatttaaa tcttcacgac tgaataattt acttaagccg ccttatgggc
     2341 ggtttttttg tgcctgccat ttatctgtgg gccacaacgt gagacacaaa ctgggacaca
     2401 gaggattttt tagggttgag tctttggctt cccgctggca tgatccagaa cagggattaa
     2461 cgggacgtta atcgcaatct tttgattttg ttttaaatga ctcggggtgc ccttctttgt
     2521 gaaggctgag aaatacccgt attacctgat ctggataatg ccagcgtagg gaagtcacgg
     2581 accaccaggt cattgcttct tcacgttatg gcaggagcaa actatgcaag tcgacctgca
     2641 gagttcagcg caatctgcgc acgcgttaca cctttttcac caacattccc ctcttgtgca
     2701 ctgcatgacc aacgatgtgg tgcaaacctt tactgccaat accttgctgg cgctcggtgc
     2761 atcgccagcg atggttatcg aaaccgaaga ggccagtcag tttgcggcta ttgctagtgc
     2821 gttgctgatt aacgttggca cgctgacgca gctacgcgca cagtcgatgt gtgccgccgt
     2881 tgagcaagca aaaagctctc aaacaccctg gacgcttgat ccagtagcgg ttggtgcact
     2941 cgattatcgc cgccgttttt gtctggaact tctgcctcat aagccaaccg ctatacgtgg
     3001 taatgcttcg gaaatcatgg cattagctgg cattgctaat ggtggacggg gagtggatac
     3061 cactgacgcc gcagctaacg cgatacccgc tgcacaaaca ctggcacggg aaactggcgc
     3121 aatcgtcgtg gtcactggcg agatggatta tgttaccgat ggacatcgta tcattggtat
     3181 tcacggtggc gatccgttaa tgaccaaagt ggtaggaact ggctgtgcat tatcggcggt
     3241 tgtcgctgcc tgctgtgcgt taccaggcga tacgctggaa aatgtcgcat ctgcctgtca
     3301 ctggatgaaa caagccggag aacgcgcagt cgccagaagc gaggggccag gcagttttgt
     3361 tccacatttc cttgatgcgc tctggcaatt gacgcaggag gtgcaggcat gaaacgaatt
     3421 aacgctctga cgattgccgg tactgatccg agtggtggtg cggggattca ggccgatctt
     3481 aaaaccttct cggcacttgg cgcttatggt tgctcagtta ttactgcact ggtggcgcaa
     3541 aatacccgtg gcgtacagtc ggtgtatcgc attgagtctg attttgtcgc cgcccagctc
     3601 gattcggtgt tcagcgatgt gcgaatcgac accactaaaa tcggtatgtt ggcggaaacc
     3661 gatattgttg aagcggtggc agaacggttg caacgttatc agatccaata cgtggtactc
     3721 gacaccgtta tgctggcaaa aagcggcgac ccgctgcttt caccttcggc ggttgctacg
     3781 ctgcgcagtc gattattgcc acaggtttca ttaataacgc caaacttgcc cgaagctgcc
     3841 gccttgctcg acgcgccaca cgcgcgcacc gaacaggaaa tgctggaaca agggcgatcg
     3901 ctgttggcga tgggctgtgg cgcagtgcta atgaaaggtg gtcatctgga tgatgagcaa
     3961 agcccggact ggctgtgtac ccgcgagggt gaacaacggt ttaccgcacc gcgcattatg
     4021 accaaaaaca cccacggcac tggttgtacg ctctctgcgg cgttggctgc actacgcccg
     4081 cgccatacaa actgggctga caccgtacag gaggcaaaaa gctggctttc atcagcatta
     4141 gcccaggccg acacgctgga agttggtcac ggtattggtc cggttcacca cttccacgcc
     4201 tggtggtgag caccgtacta tagctgcttt ggttattgtc agcctttggc actgacattc
     4261 agggatgaaa ccaatgcagc taagaattac cagtcgaaaa aaaactcacc tctctattat
     4321 gcgctcttgg gctaatttcc atcgttgcaa tttatccgcg tcagaaggtg aattttttct
     4381 attcgacagc agttcagatt acagactaca tccatttcta cggttatcgc ccggttaaat
     4441 ctttcgctat tcgcattcct gccagttaca ccattcacgg aatagatgtt tctcgctggc
     4501 aggagcggat cgactggcag cgggtggcaa aaatgcgcga caacggtatc cgcttacagt
     4561 ttgcttttat taaggcgacg gaaggcgaaa agctggtgga cccgtatttt tcgcgtaact
     4621 ggcaactaag ccgcgaaaat ggcctgctgc gcggggcgta tcattatttc tccccgtcgg
     4681 tagcagcgcc ggttcaggca cgattatttc tgcaaacggt ggatttctca caaggcgatt
     4741 tccctgcggt actggacgta gaagaacggg gaaaattatc ggcaaaagaa ttacgcaaac
     4801 gagtaagtca gtggctaaaa atggtcgaaa aaagtacggg aaaaaagccg attatttact
     4861 caggagccgt tttttatcac accaatctgg cgggctattt caatgaatat ccgtggtggg
     4921 tggctcacta ttatcaacgt cgtccggaca atgacggcat ggcctggcgc ttctggcagc
     4981 attccgaccg tggacaggta gatggcatca atggtccggt ggattttaat gtatttaatg
     5041 gcacggtgga ggagttgcag gcattcgttg atgggattaa agaaacgcct taaataagaa
     5101 gcagtgatat actcccacca gactccccgg caggaacaag acaaatggaa caagcgcata
     5161 cccagctgat tgcccaactg aacgagcgta ttttagcggc ggataacacg ccgctttata
     5221 ttaagtttgc cgaaacggta aaaaatgccg tgcgcagcgg ggtgctggag catggcaata
     5281 ttttgcccgg tgagcgtgat ttaagtcagt taaccggcgt ttcgcgcatt acggtgcgca
     5341 aggcgatgca ggcgctggaa gaagagggtg tggtgacgcg ttcgcgcggt tacggcacgc
     5401 agatcaacaa catcttcgaa tactcgttga aagaagcgcg aggtttttct cagcaggtgg
     5461 tattgcgcgg gaaaaagccc gatacgctat gggttaacaa gcgtgtcgtg aaatgtcccg
     5521 aagaagtcgc gcagcagctg gcggtcgaag caggaagtga tgtctttttg cttaagcgta
     5581 ttcgctatgt cgatgaagaa gcggtatcga ttgaggaatc gtgggttccg gcgcatttaa
     5641 ttcatgatgt tgatgccatc gggatttcac tttatgacta tttccgcagc cagcacattt
     5701 acccacaacg tacgcgttcc cgcgttagcg cccggatgcc ggatgccgag tttcagtcac
     5761 atattcagtt agatagcaaa ataccggtgc tggtgatcaa gcaagtggcg cttgaccaac
     5821 agcaacggcc tattgagtac agcattagcc actgtcgcag cgatctatac gtttttgtgt
     5881 gcgaggagta gttcctcgcg cgttggcgca caatcaccgc cccgatgccc gacaacccat
     5941 gacgccactg cattgcccag taatacggca tccgccagtg gcagaccaga ggccagcccg
     6001 gcaagtacgc caccggcatg actgtcgccc gccccaatgg tgtcgacaac ttgcgttgga
     6061 aatgccggaa tgcagccaga agcgtcgttg ctgaaatacc atgcgccttc tttatcgaga
     6121 cgaacgatca acggcgcggc aaatttctcc tgccattgct tgccaagtgt tgttatctct
     6181 gcggataaag caaaacgttc ggcggcaatc tcagcctctt gacgattgag tgacactaaa
     6241 ggtcgacagg ccatgatccg cgccagtaat gcgtccggga tatcgccaat acgtgggcca
     6301 aaatcgataa acggcgtcac gtcttgcagc tcttccagcc attccactaa caattcgccg
     6361 cagggcgagg ccagttgata accggaaaaa tagagcaggc tgccaggcgc aacggttaat
     6421 cgcgccagcc actggcgatt ccactgattt tccacaccgc tgaatgacat aaaagtacgt
     6481 tcgccatccg gctcaaccag cgccagacac cagccgttat cgccttcggc gttatcgatc
     6541 aggctgatta acccctcttt cgccatccgg ttgcgaataa tctccgccca taccccctga
     6601 ccgagcggca aggcattacc cgcttcgatg ccgaggcgct ttaacgccac ggcaatattc
     6661 agtgcgcagc cgccaacgtt aacgctctgc tgtttcagtt cgatatcgca cccacgccag
     6721 gggagggcat aagcgtcggc gatcacgtca atgaccgccg cgccgacgac catcaccgac
     6781 tgacgcgtgg ttaattcagg cagcagcgtg tgtaatcgag cgccgctcat accgcctccc
     6841 gttgttgacg atatttcgcc agcgctgtgg catagcggtt gaaatcaagc tgatttaccg
     6901 catccagctc cgcctttaac gcaggatcga tagcgttaat gccatgcaac gcgccgcaga
     6961 ttgccgtcgc catagcaccg atggtgtctg tatcgccgcc aaggttagcg cagaggacgg
     7021 cgcaacggtt cgggtcggtt tgtgccagtt caaccagcgc aatggcgcac ggaacggact
     7081 caatagtgct ggtacctgcg ccaacgacct ggtaaagctg ttcgctggcg gattcggtgc
     7141 cgtcggcatt gcgcacaatt ttcagcgcaa tctccagccg cgccgccagt gaggcgctga
     7201 acgtggtggt gcgtttttgc tgggcatgtc gcgtaattga aggcagtgaa tcaacaatcg
     7261 ctgaccagct ttctccgtca atagcacgag aaatcgccca tgcgatgact accgcacctg
     7321 caactgccag atcggattta tgggtcgggc tcgacgccag cgccacatca tcaataaagg
     7381 aatcaacatc gcgcgccgga agcaaacaac ctaatggcga gacgcgcatc gctgcgccgt
     7441 tggttacgcc gttgttttcc agttcagcaa cgggtttacc gtcgcgaatg gcgttaagcg
     7501 caatcttcga ggtcggacct agcacgtttt tgttgaaggc gtcgaaacgc agcgcccagt
     7561 cgaggatatt acgcccaatc agatccggat cgatcttgcc tttacgttcc agcaacgcat
     7621 ccgccagaca cagcgccatc gaggtatcgt cggtgaattc ggcgcggtta aaataacagg
     7681 ccgcgttatt ctcctttggt ccaggcagaa aacggtcaat ccagccaaag tgtgctttaa
     7741 cgcggctgcg tggccaaagc tcggagggca tccccatcgc atcccctaac gcctgcccat
     7801 aaagagcacc gagaatacgt tctgttttca tttaacttcc ccttgtgtca acgcaatatc
     7861 gcgatcatcg accttgatag ccgtaatttc gttgtcggat tcgcagaaaa aaatcatgaa
     7921 cagcacggcg ataatggcaa tcatcaccgc gccaaaagtc cacatcccgg accagttgaa
     7981 agtcagtccg tttaccggtt cctgataagc gaacatcttt tccatcatca caccgccaag
     8041 acgatagccg agcaaactgc cgaagccctg gcagcagagc gtgatcagcc cctgcgcggc
     8101 ggtacgcata tgcacggggg cttttttatc gacatagatg taagcggtaa cgtagtaaaa
     8161 atcgtaactt acgccgtgaa gcaaaatacc gaggaacagt aacgcgtagg tgaaatattc
     8221 atccgcacta ccgtaaataa agaagccata gcggatcgca gcgatgacca gaccaagcaa
     8281 taataccttt ttgataccaa agcgtttagt gaaaaacggt aatgccagca taaagaatat
     8341 ttcagagaac tggccgagcg tcatccagcc ggtggcattt ttcatgccga cttcggtcag
     8401 ataaccgttg gcaaagatgt aatagaacgc cagtggcatc gcaaacagga atgaacagaa
     8461 gaaaaagacg aggaagtttt tatcgcgcag caggatcagc gcatccaggc cgagcatgac
     8521 tttaatatcc attttgccgg tgctttttgg tggcgtgccg ggcaggaaaa acgcaaacac
     8581 accgagcaga gcagaacttc cggcggtaat cagcagcggg atgttagtcg gtgagatatc
     8641 ggcatacccc agtatttgcg gcaagaaacc acatgccaga ccggaggcaa tccagccgat
     8701 agtgcccatc acacgaatgc gcgggaaatc acgctccaca tccggcacgt tggcaaaagc
     8761 gatgctgtta gtcagcgcaa tggtcggcat ataggttaga gagtaagcca gcagtaacgg
     8821 aaagaacccg gcaaaagtgg tctgttgcgc agcgaaatac atcagcagcg cgcctgcgaa
     8881 catcaatacc gccagcactt tttgcgccga gaaaaagcgg tcagtgatgg agccaaccag
     8941 aattggcgac aggatcgccg caatggcggt acaggcatac gaccagccaa tttctccggc
     9001 actaaaaccg cttttactta accacaacca caatggcaca aaccacgcgc cccagataaa
     9061 ccattcaaca aacatcatga acgacagctt tgctgttgtt ttcatctttt aattccttca
     9121 tgacaggtaa tgtaaggtac gcgatgacaa taccacttaa taataccttt taaatatctt
     9181 tacggtgaaa acttgaccgc gctaacattt tttctgatga atcgagccaa cagaaaacgc
     9241 tgaaaaaaca tccaaaagat ggaaaaactc gcccgtaacg accaggatta tgattgcccg
     9301 caaaaggcgg gccaggacaa tccatagccg atatccaatc ggaatttacg ggagcatagt
     9361 aatgacagat attgcgcagt tgcttggcaa agacgccgac aaccttttac agcaccgttg
     9421 tatgacaatt ccttctgacc agctttatct ccccggacat gactacgtag accgcgtaat
     9481 gattgacaat aatcgcccgc cagcggtgtt acgtaatatg cagacgttgt acaacaccgg
     9541 gcgtctggct ggcacaggat atctttctat tctgccggtt gaccagggcg ttgagcactc
     9601 tgccggagct tcatttgctg ctaacccgct ctactttgac ccgaaaaaca ttgttgaact
     9661 ggcgatcgaa gcgggctgta actgtgtggt atcaacttac ggcgtgttgg cgtcggtatc
     9721 gcggcgctat gcgcatcgca ttccatttct cgtcaaactt aatcacaacg agacgctaag
     9781 ttacccgaat acctacgatc aaacgctgta tgccagcgtg gagcaggctt tcaacatggg
     9841 cgcggtggcg gttggtgcga ctatctattt tggctcggaa gagtcacgtc gccagattga
     9901 agaaatttct gcggcttttg aacgtgcgca tgagctgggc atggtgaccg tgctgtgggc
     9961 ctatttgcgt aactccgcct ttaagaaaga tggcgttgat taccatgttt ccgccgacct
    10021 gaccggtcag gcaaaccatc tggcggcaac catcggcgca gatatcgtca aacaaaaaat
    10081 ggcggaaaat aacggcggct ataaagcgat taattacggt tataccgacg atcgcgtgta
    10141 cagcaagtta accagcgaaa acccgattga tctggtgcgt tatcagttag ctaactgcta
    10201 tatgggccgg gctgggttga taaactccgg cggtgctgca ggcggtgaaa ctgacctcag
    10261 tgatgcagtg cgtactgcgg ttatcaacaa acgcgctggc ggaatggggc tgattcttgg
    10321 acgtaaggcg ttcaagaaat cgatggctga cggcgtgaaa ctgattaacg ccgtgcagga
    10381 cgtttatctc gatagcaaaa ttactattgc ctgatacgcc ttatacgaac aacaccaccc
    10441 cgtaggacgg ataaggcgtt tacactgcat ccggcaacct atgcctgatg cgacgcggaa
    10501 gcgtcttatc atgcctacat agcactgcca cgtatgttta caccgcatcc ggcataaaaa
    10561 cacgcgcact ttgctacggc ttccctatcg ggaggccgtt tttttgcctt tcactcctcg
    10621 aataattttc atattgtcgt ttttgtgatc gttatctcga tatttaaaaa caaataattt
    10681 cattatattt tgaaaacgaa aacaaacgac aggatatgaa aatgcacgtg gtatcgacaa
    10741 agcagatgct gaacaacgca cagcgcggcg gttatgcggt tccggcattc aatattcaca
    10801 atctcgaaac gatgcaagtg gtggtggaaa ccgctgccaa cctgcatgcg ccggtcatca
    10861 tcgccggaac gcctggcaca tttactcatg ctggtacaga aaatctgttg gcgctggtca
    10921 gcgcgatggc gaagcaatat caccatccac tggcaattca tctcgaccat cacacgaaat
    10981 ttgacgatat cgctcagaag gttcgttctg gcgtgcgctc agtcatgatt gacgcctcgc
    11041 atttgccttt tgcgcaaaat atttcacggg tcaaagaggt ggtggatttt tgccatcgct
    11101 ttgatgtcag cgtcgaagcg gagctggggc aacttggcgg ccaggaagat gatgtgcaag
    11161 tcaatgaagc cgatgctttt tataccaacc ccgctcaggc gcgtgaattt gccgaggcaa
    11221 ccggaattga ttccctggcg gtcgccatcg gcacggctca tgggatgtat gccagcgcac
    11281 cggcgcttga tttttctaga ctggagaaca ttcgccagtg ggtgaactta ccgctggtgc
    11341 tgcatggcgc gtcagggtta tcgactaagg atattcagca aaccatcaaa ctggggatat
    11401 gcaaaatcaa cgttgcaacg gagctgaaaa atgccttctc gcaggcgtta aaaaattacc
    11461 tgaccgagca ccctgaagcg accgagcccc gggattattt gcagtcggct aaatccgcaa
    11521 tgcgcgatgt ggtgagcaaa gtgattgccg attgtggctg cgagggcagg gcataacgca
    11581 cctgccattt aataaggaaa aaacatgaaa acgttaattg cccggcataa agctggtgaa
    11641 catatcggca tatgttcagt ctgttctgcc catccgttgg ttatcgaagc ggcgctggca
    11701 tttgatcgca acagcacgcg caaagtgctg attgaagcaa cgtcaaacca ggtcaatcaa
    11761 tttggcggtt ataccggaat gacaccggca gactttcgcg aatttgtttt tacgattgcc
    11821 gataaagttg ggtttgcacg cgaacgcatt attctcggcg gcgatcatct ggggccaaac
    11881 tgctggcagc aagaaaatgc ggatgcggcg atggaaaaat ccgtcgagct ggtaaaggaa
    11941 tatgttcgtg ccggcttcag taaaattcat cttgatgcgt caatgtcctg cgcgggggat
    12001 cccataccgt tagcaccaga aacggttgcg gaacgagctg ctgtgctttg ctttgctgcg
    12061 gaaagtgtgg cgacagattg ccagcgtgag caactgagct atgtcattgg caccgaagtt
    12121 ccggttccgg gcggtgaggc cagcgccatt cagtcagtac acatcaccca tgttgaagat
    12181 gccgccaata ctttacgtac gcatcaaaag gcctttattg tccgtgggct gacagaggcg
    12241 ttaacacgcg tgattgccat cgtggtgcag ccgggcgtgg aatttgatca cagcaatatt
    12301 atccattatc agccgcagga agcgcagccg ctggcgcaat ggatagaaaa cacccgaatg
    12361 gtttatgaag cacattctac cgattaccag acccggacgg cttattggga attagtccgc
    12421 gatcactttg caatattgaa agtcggtccc gcattaacct ttgctttacg cgaggcgata
    12481 tttgcactgg cacaaattga gcaggaactt atcgcccctg aaaatcgcag cggttgcctg
    12541 gcggtaattg aagaagtgat gctcgacgaa ccgcaatact ggaaaaaata ttatcgtacg
    12601 ggttttaacg attcattact ggatattcgt tacagcctgt cggatcgtat tcgttattac
    12661 tggccgcata gtcggattaa aaatagcgtc gaaacgatga tggtgaatct tgaaggcgtg
    12721 gacatcccac tgggcatgat tagtcagtat cttcccaaac aatttgaacg cattcagtcc
    12781 ggggaattat cagcaatacc gcattagctg attatggata aaatttatga tgttttgcgc
    12841 gcctatcgct acggctgtgc ggaataagga cggtatatga ctaacctgtt tgttcgtagc
    12901 ggaatttctt ttgtcgatcg tagcgaagtt ttaacccata tcggtaatga gatgctcgcc
    12961 aaaggtgtgg ttcatgatac ctggccacag gcattaattg ccagagaagc agaattccct
    13021 accgggataa tgcttgagca gcacgccatt gcaataccgc attgtgaggc gattcatgct
    13081 aagtcgtcag ccatttatct gttaaggcca acaaataaag ttcattttca gcaagcggat
    13141 gatgataacg acgtggaggt atcgttggtt attgcgttga ttgtggaaaa tccgcagcag
    13201 caattgaaac ttttacgctg tttatttggc aagttacaac agcccgatat cgtcgagaca
    13261 ctaatcactc ttcctgaaac ccagttaaag gaatacttca caaagtatgt tttagattca
    13321 gacgaataaa tccctctgta acaataaaaa ggactattta tgaaacgcaa gattattgtc
    13381 gcttgcggag gcgcggttgc gacctctacg atggcggcgg aagaaattaa agaattgtgt
    13441 cagaatcata atattcctgt tgaattaatc cagtgtcggg ttaatgaaat agaaacctat
    13501 atggatggcg tgcatttgat atgcactact gccagggtgg atcgtagttt tggcgatatt
    13561 ccgttagttc acggcatgcc ttttgtttct ggtgtcggta tcgaagcatt acaaaataaa
    13621 attctgacta tcttacaggg gtgacctatg ttttcagaag tcatgcgtta tattctcgac
    13681 ctcggcccta cggtgatgct gccgattgtc atcattattt tttctaaaat attaggcatg
    13741 aaggcaggcg attgctttaa agcgggtctt catataggga ttggctttgt tggcattggc
    13801 cttgtgattg gcttaatgct ggattccatt ggtccggcgg cgaaagcgat ggcggaaaat
    13861 ttcgacctga atctgcatgt ggtcgatgtc ggctggccgg gctcttcacc aatgacctgg
    13921 gcgtcgcaaa ttgcgctggt ggcgattccg attgctattc tggttaacgt ggcgatgtta
    13981 ctgacccgta tgacgcgggt ggtaaatgtt gatatctgga atatctggca tatgactttc
    14041 accggcgcgt tgctgcatct ggcaaccggt tcatggatga tagggatggc gggtgtggta
    14101 attcacgcgg cgtttgttta taagctcggc gactggtttg cccgcgatac ccgaaatttc
    14161 tttgagctgg aaggtattgc tattccgcac ggtacgtcgg cgtatatggg gccgattgcg
    14221 gtgctggtcg atgctatcat cgagaaaatc ccaggcgtta accgaattaa atttagcgcc
    14281 gatgatattc agcgcaaatt tggccctttt ggcgaacctg tcaccgtggg ttttgtgatg
    14341 gggctgatta tcggcatcct cgcgggttac gatgtcaaag gtgtattgca gctggcggta
    14401 aaaacggcgg cagtgatgct gctaatgcca cgggtgatta aacccatcat ggatggttta
    14461 acgcccatcg ctaagcaggc ccgtagtcgt ttacaggcga agttcggcgg tcaggagttc
    14521 ctgattggcc tggatcccgc attactgctg gggcatacgg cggtggtatc ggtaagcctg
    14581 atttttatcc cgctcaccat tttaattgct gtttgtgttc cgggtaatca ggtactgccg
    14641 tttggcgatc ttgccaccat cggcttcttt gtggcgatgg cagtcgccgt gcatcgtgga
    14701 aatctgttcc gcaccttaat ctcgggtgtc atcatcatga gcatcactct gtggatcgcg
    14761 acgcaaacta ttggtttgca cacccaactg gcggctaatg ctggggcgtt aaaagccggg
    14821 ggtatggtgg cttcaatgga tcagggcggt tctcccatta cctggttact gattcaggtt
    14881 ttctccccgc aaaatattcc cggtttcatt attatcggcg caatttatct gaccggtatt
    14941 ttcattacct ggcgtagagc gcgtggtttt attaaacaag agaaagccgt tctcgcagaa
    15001 taatttttaa ctgaggggga gtttatcccc cttaacaatc aggagttttt atgaaatcag
    15061 tggtgaatga tactgatggt atcgtgcgcg ttgcagaaag cgtcattcct gaaattaaac
    15121 atcaggatga ggtgcgggta aaaattgcca gctcgggctt atgtggttcc gatttaccca
    15181 ggatatttaa aaatggtgca cattattatc caataacgtt aggccatgaa tttagcggct
    15241 atattgatgc ggtgggatcc ggtgttgatg atttacaccc tggcgatgcg gttgcctgtg
    15301 tgccgttatt accctgtttt acttgtccag agtgtctgaa agggttttat tcccagtgcg
    15361 caaaatatga ttttattggc tcgcggcgtg atggtggatt tgctgaatat attgtcgtta
    15421 agcgaaaaaa tgtctttgct ctacccacgg atatgcctat tgaggatggg gcttttattg
    15481 agccgattac cgttggtctg catgcttttc atttagcgca aggttgtgag aataaaaacg
    15541 ttattattat tggtgccgga accattggcc tgctggctat tcagtgcgct gtcgcgctgg
    15601 gagcaaagag tgtgacggct atcgacatta gctcagaaaa actggcactg gcaaaatctt
    15661 tcggttcgat gcaaacattt aacagtagcg aaatgagcgc gccgcaaatg cagagcgttt
    15721 tacgcgaact gcgttttaat cagcttatcc tcgagacggc tggcgtaccg caaactgtcg
    15781 aactggcggt agagattgcc ggtcctcatg cccaactggc gctggtgggc acgttgcatc
    15841 aggatctgca tttaacatcg gcaacgtttg gcaaaatatt gcgtaaagag ctgacggtta
    15901 tcggcagttg gatgaactat tccagccctt ggccggggca ggagtgggaa acggcgagcc
    15961 ggttgctgac agaacgtaag ttaagcctgg agccattaat cgctcaccgt ggaagctttg
    16021 aaagcttcac ccaggcggtg cgtgacatcg ctcgtaatgc catgccgggc aaagtgttgc
    16081 tcattccatg aaaccgcggg ccagcgtgat gctggcccgg tattgtgcaa aacagatcat
    16141 tcaccaatgg tcccccttcg tttacactag ccacaattga atatgggtaa atgacgatga
    16201 attcattcga gcgaaggaat aagatcatcc aattagtgaa tgaacaggga acagtgcttg
    16261 ttcaggatct ggcgggagta tttgctgcct cggaagcgac aatccgtgcc gatttgcgct
    16321 ttctcgaaca aaaaggcgtg gttacgcgct ttcatggcgg tgcggcgaaa ataatgtccg
    16381 gtaatagtga aaccgagacc caggaagtcg ggtttaaaga gcgatttcag ctcgccagcg
    16441 cgccaaaaaa cagaatagcg caggcggcag tcaaaatgat ccatgaaggg atgaccgtta
    16501 ttctcgacag cggaagtacc acaatgctta tcgccgaagg attaatgacc gctaaaaata
    16561 tcacggtgat taccaacagt ctcccggcag cgtttgccct ttccgaaaat aaagacatta
    16621 ctctggtcgt ctgcggtggc acggtccgcc ataaaacgcg atctatgcat ggatctattg
    16681 ctgaacgttc cctgcaggat attaatgccg atttaatgtt tgtcggtgct gatggtattg
    16741 atgcggttaa tggtattacg acctttaatg agggttattc cgttagcggt gcgatggtca
    16801 cagccgctaa taaagtgatt gccgttctcg attcatcgaa atttaaccgt cgcggtttta
    16861 atcaggtact gccaatcgaa aaaattgaca ttattattac cgatgatgct gtatcagagg
    16921 tggataaact ggcattgcag aagacacggg taaaattaat tacggtgtag tattgttctg
    16981 tatgccggat aaggtattta caccgcatcc ggcatttcgg attctccctg gttcaagaga
    17041 gaatagtttg attaacgcaa taatggacaa tctggtggta atcgacaacg caacgccgcc
    17101 ggaagtattt caatatgaaa gttttgtccg ctcaacggtt cgccatcaag attaaaagtg
    17161 atttcgtgtg gggcttgtat atcaaaccac gacgaagcgc cttcgataat actcgggtta
    17221 tcttcgtcag attttaaggt tgatacgaga gccggaagaa tttcatcgcc ggtaaaaatg
    17281 cgcagttgca gcaagccatc gttaattaac gcgttcggac acaattgctg accgccaccg
    17341 gcctgacgcc cgttaccaat accaatacca gggcgtcacc ttgccagtga aagttttcac
    17401 cgcggatttc acaacggtcc ggttgcagag tatccatgcg cattaagcca tgaatgatgt
    17461 aagagacgcc acccagcgcg gcttttaatt tttccggcgt ttctgtggta atacgcgtcc
    17521 caaatccgcc tgtcgccata ttaataaaac aggtttgttt gttgacctgc gccatatcta
    17581 tcgcaatggc gttaccggca attgccagtt tcagcgcctt atccagtgcc tcaggaatcc
    17641 ctacactggt ggcaaaatca ttggcggttc ctaatggcaa aattcccagc gcgggtatgc
    17701 catccccctc acactgaatc aacgccgtag aaacttcatt aatggtgcca tcgccaccac
    17761 cggcaattac cgttgcgacg cccaacttcc gggcctcctc tacaaatcgt gcggcatcac
    17821 ctttctccca ggtgacccgc acatggatcg tcattccttc ctcacgcaac agcataattg
    17881 cttcgcgcaa gggtagattg tcagtacttt tgccattaag aatcagtaag ctggcgggaa
    17941 attctgccat gatcgtatgt gcctttatga ttggtctgta gatagtgtag agcagaaaag
    18001 aaaaggtgga gtcagaacag gatgaaagtc ggaggataaa aagtcagttc aagtatggag
    18061 gattcagtgg ttaccaccag gagcttgcaa tgggaacagc gaatattaac ggtagccacc
    18121 gataaatttc aaaaaagagc atatgcctaa tattcaacca aacagtggca tcttaaatat
    18181 actattccaa atatattaaa gccccatgga gttacctgga aagggccttc atgtctgtaa
    18241 gtcctactta tgtaggaaat gttgtacaga acatttatta taatcctatt caaatataat
    18301 gatcagctat tattatattt aaacactaga gagtgtcgtt ggtatttaat ggggaaaggt
    18361 gagatgaaaa agatagctgc tatatcatta attagtattt ttattatgtc tggttgtgct
    18421 gtgcataatg atgagacaag tatcggtaaa tttggtcttg catataaaag taatattcag
    18481 cgtaaactcg ataaccaata ctacaccgaa gccgaagctt ctttagccag gagtagaata
    18541 tctggtgcag aaaatatagt aaaaaatgat gcaactcatt tctgtgttac tcagggcaaa
    18601 aaaatgcaaa tagttgagct gaagacagaa ggtgtaggat tacatggtgt cgctcgtctg
    18661 acattcaaat gtggagagtg agaatatttt ttggtaagca tcaaacatgc gcgttctggt
    18721 tgtgcttagc cggaacctgt gcgagcacga tgccgttacg tgaaaggcat cgtgctatga
    18781 agggagattc tatcgatgtg gtcaatggaa gacggtgacc agggataggg cttatgcata
    18841 aaaaataagc ccgtgtaagg gagattacac aggctaagga ggtggttcct ggtacagcta
    18901 gcattttatg ggttatgttt ttcagcgaaa cggatgataa ccttaataaa tgcagctgta
    18961 tgtgatcggt ttctaagaat tttccatccg ggaaaaataa tcgaaattaa tcacttaccg
    19021 tgtgggttac gcgtggtttc cccggagaaa ttacgcatca gcagcgcgta atttagttca
    19081 agatcctgcg ggaccgggag ccacacagta taaccatcgc ctggtgcgac cggcatcgct
    19141 acacctttgg cgttttccat gtgctcaagg gtaaagttaa tgttgccttg cggcgtcatc
    19201 agctcaaggc tgtcgccaac ggagaattta tttttcaccg ctaccgctgc gaggtccccc
    19261 ttgcgctcac cggtaaactc accaacaaac tgctggcggt cagaaactga ataaccgtat
    19321 tcgtagttct gataatcgtc gtgagtatga cgacgcagga aaccttcggt atagccacga
    19381 tgcgccagac cttccagagt ttccagcagg ctggtatcga acggtttgcc cgcagcggca
    19441 tcatcgatag ctttacgata aacctgtgcg gtgcgtgcgc aatagtagaa agatttagta
    19501 cggccttcga ttttcagcga atgcacgccc attttggtca ggcgttctac atgggcgatg
    19561 gcgcgcagat ctttcgagtt catgatgtaa gtgccgtgct catcttcaaa cgcggtcata
    19621 tactcgcccg gacgctgggc ttcttcgatc ataaacactt tgtcggttgg cgcgccgata
    19681 cctagcgtcg gctcaacatt ttgcaccggg atcggctcgt acttgtgtac gatgttgcca
    19741 acgtcatctt ctttcccttc ctggacgttg tactcccagc ggcaggcgtt ggtgcaggta
    19801 ccctggttcg gatcgcgctt gttgatatag ccagagagca ggcagcgacc ggagtaggcc
    19861 atgcacagcg caccgtgaac gaagatctcg atctccatat ccggcacctg attgcggatc
    19921 tcttcaatct cttccagcga cagttcgcga gagaggatca cgcgggtcag gcccatttgc
    19981 tgccagaatt tcaccgtcgc ccagtttacg gcgttagcct gcaccgagag gtggatcggc
    20041 atttcaggga agtactcacg caccagcata atcagccctg gatcggacat aatcagcgca
    20101 tccggcccca tttccaccac cggtttcagg tcacggataa aggttttcag cttggcgttg
    20161 tgcggtgcaa tgttgaccac gacataaaac tttttcccca gcgcgtgggc ttcattgatg
    20221 ccgagctgaa gattttcgtg gttgaattcg ttgttgcgca cacgcaggga gtaacgcggc
    20281 tggcccgcat aaacagcatc tgcgccataa gcgaaagcgt aacgcatatt tttcagcgtt
    20341 cccgccgggg aaaggagttc cggtttaaac ataattctct cgttctgatg acaggtcaga
    20401 tccgcttcac ctgatgaagc ggttagggga gtgcccccac tttaagggcg ggcattgtag
    20461 cgcaatcggc ggggttggct ccagtgcaaa tgtgagggaa aagactgggg gatttatttg
    20521 ttaccggcct tcactttatg taaaaacaga tccttttccc acggttgggc ttcgccactg
    20581 cgtagtccct cggcaagttt ttctcgtagc aagcgtaaag cttcacgtat tgtttcgttt
    20641 tggttccgct cgtcagcagg tacatttgtc atcgtaagtc tataattcga agttatttaa
    20701 aaggaatgta accccataat ctgttgtgaa gttccggatt gcgggtaaaa agcgcctgaa
    20761 gttgctgacg ctgaagagca ttcggttgaa tgcccgaaag attgaaattg cgttgccata
    20821 tcgcgagctg tttacctgca aattcttcag ttagcttgtg ttgcgattga agaagaagaa
    20881 ttaaaccgtc ccatttgttc aagttatgat acagccgaca atatgcgcaa gcatgtttaa
    20941 ctgacggcgc gttttcctgc aactgacgta tttcctcgaa cgtgagcgga acgcgacacc
    21001 atacacagat gttaaccata aatcgcaacc cggcggcgtc cgggttagcc agcgtggtca
    21061 gaatagtctc tttgacgcca caacgaggat tagcttttgc aagaatgtgc atagcggcta
    21121 aacgcagtgg gaatacattc tgtgataatc cccattctgc cagttttaaa atattgggat
    21181 catgaatgga atccagcccg tataaagcgg cccgtcgctg tgagggagta tattccgcag
    21241 aagtgacaac atgacgccag aattgcggaa catccatgac ttcgtgaagc atatttttag
    21301 cgcgtaggcg gatcaacata ttgttatcca gcagtaatgt gctgtgcaac gccggaggca
    21361 tttctattgc tcggtcagtg aggctaaata acgttgcttg tcgaatacgc ggccagcggt
    21421 cttttaacag acgagttaat accgcttcgc ttaatggtaa ttgttgacgc agccagtggg
    21481 tggtcgccag cattcgcaaa ccctcatcgg gtttatcgag tattgaaagc agtgaagatt
    21541 catcattaaa gtaaccgtat tcaatcagcg tttgtaatac cgcgcgggag agctttttat
    21601 cagggcaagt aagccaatac gttaattgtg ggcggttatt ttctgtcaat aaatatctaa
    21661 gtatctcgtc tacaaatgca cggtgatcgt aacgctggca ttggagaagg ttacgtatca
    21721 tgggtagatt aacggcaaat gccgctgcat tcttatcctt cattaaatga cgtaatgcta
    21781 tggttgcagc aaggcgaacc tcttttaccc agtcattagc tgcaagtaat aattcttcta
    21841 tagccgcaac atcctctttg tgccccagat actgcacggc cttttctcgt atatatccat
    21901 tgtgatgatg ggtaagctgg caaagaacca gagtatcgtt gagatcacaa tcatgtgcta
    21961 aataattgcc tggttcggct atcaatgcct ggagttgttg ctggatgaga agtaattcac
    22021 agtcaacaga attattcatt tcgacgttaa acaaaaccct gcgtgaaaat aatgtacgca
    22081 atcctttgag cataattttc cttatcagtt atgaggagaa aactcatttc atggttaata
    22141 atttcttgag ggatagtaac tctgtgatga gagaaaataa agccggacgc caacaaccca
    22201 cgcccggcat cgtaattacg tattgtcttt caccgtctgg cttgtgccat tggctttaac
    22261 ggaggcaatt ccggtttcac gagattgcgc ggtggcgtac atctggctgg agccgataat
    22321 ttgatgattg gcggctttca gattgaaata gaatttgccg ttacttgccg tttttttctc
    22381 atagcgttct tcttgcgggc tgttgctacg cactgacgcg atgccctttt ccgcagaggc
    22441 ttttgatgta tacagctcgc tggtgaggat agtctcacca ttgcccgctt ttagcacaaa
    22501 ccggaactga ttatcactgc tcttactgag ttcaaaccaa ccagccataa tgactccttg
    22561 attgaaattg cgtatcgaaa acagcatcta aaggtgctgt ttaccgatta aagtatgagc
    22621 gattttctga tgcctgccag ggcggagaga taatgtcagt ttgctgacca tgcgtgggat
    22681 aatgttgccg gatctggtct gaacgccatc tccggctaac aaagtcatgt cgctaaaact
    22741 aaacgatgcg gcaggcgtcg gcttcccagc ggtaaccaac gccgtaaacg gcgcggataa
    22801 atgactgttc ggcgtcgaga gattccagct tgcggcgcag gtttttaatg tggctgtcaa
    22861 tggtgcggtc ggttactacg cggtagtcgt cataaagatg attgagcaat tgctcgcggg
    22921 agaacacttt tcctggttca tgagagagcg ttttcaacag acgaaattcc gcaggtgtca
    22981 ggtcaagcat tttaccgcgc catgaagcct gaaaacgacc ttcgtcgata atcaacgggc
    23041 tttcggcatc ctgttgctgc aattcgcgct gcggtttgca acggcgcaaa atggttttga
    23101 cgcgcgctac cacttcccgt gggctgtacg gcttgcagat gtagtcatct gcgccaatct
    23161 ccagtccgag caggcggtcg atctcttcga tttttgccgt caccatcacg ataggaatgt
    23221 cagaaaaacg acgaatttcc cggcacagcg tcaagccatc ggtgccaggg agcatcagat
    23281 ccaacaggat cagatccggt ggtgtctggc gcacatacgg cagtacctga tcgccgtggc
    23341 tgataagcgt cggcgcatag ctcgcagcac gcagataatc aatgagcaac tgccccagct
    23401 tcggttcatc ttccacgatc aaaatacgtg gtgtgttttc gtcgattggt aactcggtca
    23461 tacttctctc tgtaaatccc gttccagcgg taactctact gtaatgctta ccccgccaaa
    23521 aggcgaatgg gcagcaataa tgcggccatt atgtgcttca acaatgttca ggcaaatcgc
    23581 cagccccagc ccggaaccac cgctggcgcg gttgcgggaa ccttcggtgc gataaaaacg
    23641 ttcaaataat ttttgtagct gctcgtcact gacacctggc gcactgtcgg caaaggtcag
    23701 gcgcaccgtt ttgtcgtgct gctcggcaga gattttcagg ctgccgccgc tgtcagtata
    23761 gcgcaggctg ttttcaagta aattattgaa taactgcatt aaacggtcgc gatcgccaaa
    23821 tacggtaata ctgtctggca gggaaaattg cagtttcagg ccacgactgg cgaatcgttc
    23881 gcgaaatgcg ccgcccgcca cttccagcag tgggatcaaa tctaccggtg ctttttgata
    23941 ggcgagagcg ccttcatcag acatcgacaa ctgatggaga tcgtcaacca gtttggtcag
    24001 tgtaccgacc tccgcctgta aagacgccac cgtctccggc gtgaatttac gcacgccatc
    24061 ctgaatggct tccagttcac cgcgcagcac cgctaatggc gtacgcagtt cgtgagaaat
    24121 atcggccata aaatcgcgcc gcatttgctg gtttttctcc agtgtgctgg caagctggtt
    24181 gaagtcttgc gccagtttgc ccagttcatc ttcactggtg ggcgttacgc gggtagtgaa
    24241 atcgcccgcc gccagtttgt gcgtgccatc gacaagtcgt tttaccggtg ccagtaaacc
    24301 gcgcgccagc agaaaagtgg caagtgccgc gagtaacgtt gccagggcga caatcaacca
    24361 gctggtttgc cgctgttgtt tatcgaaatt gatatcagta ttgcgcgtta accgctcaac
    24421 gggggaggcg atcaccgcgc caacttccgc accgttgacc agaatgggtc gccgtgtacc
    24481 gtcaggtgga atcggcgctc gcggaccaac cagcactttg ttgttttgat caaccaccca
    24541 gaactgggta cgccagccgt gcggtggcat acccgggccg ggtttatctt ccgaattatc
    24601 gtgttcaaat gaacgcagga tctgaaagac aaagcgatca ttgttgcgca ggaagcgcca
    24661 gttgccatgc tgcgcatact gctcgccaag cgcatcactt aacagttgta atcgctgttc
    24721 attaccatgc ttgatgtaat caataaagcc acgctcaaaa ctgatacgca ccgcccagtg
    24781 catactgatc agcaagacaa tgcaggtggc gaaaatcgcc agaaacagtt tgccggtaat
    24841 accgggtcgc cagaacttca ttgcgcgctc ctttttcgcc gcgaaatagc tacattttga
    24901 tgcgtatcgt tcggcactct ggcaaagatg aacgccggaa gggcgatgat caacgccatg
    24961 ctaagccagg tgtacataaa gacggtttgt gtggtgccgc tgtcgacgct gacatgctgt
    25021 gaaccaaaaa gtcccagcaa cagcccggcg atagtgacgc cgatactcat cgacaattgc
    25081 ataatcatcg acagcaggct gttgccgctg ctcgccagat tgtccgggag atctttcagc
    25141 gtcagggtgt tcatggagga gaaacgcgtc gagttgacca tcccttgtaa aaacaggacg
    25201 aacggcaaaa cgtagtacca gcccagcagg gcggtagtca taaacaacag ggtgaccagc
    25261 gacagaccca gcgtggtcgc taccagtacc cgacgataac caaagcgatt caccacctgt
    25321 accacaattc gcttcattcc catgctgcca agcaccatcg ggatcatcat cagtccggca
    25381 tgaaacggcg agaaaccgag gccaatttgc aggaaaaccg gtgtcataaa gggcaacatg
    25441 ccactgccaa tacgtccggc aaagctcccc gccaggccca gcgaaaaggt acgagtacgg
    25501 aacagtttca gactgaacag ggcacggttg ttatttctgg cgtgcagcag ataaagcacc
    25561 agtgccacca cgccaactgc gaccaggcca gcaatcgcca gcggcgataa acctgtacct
    25621 ttactgccgt ccagcgccag gggttaatac cgccatgcca accgccagca ataaaaatcc
    25681 ggagagatca aagcgccacg tctgcatggt gtagttcggc attaacagca atgtggcgat
    25741 cgcaccgata atccccaccg gaatgttgat caaaaagatc cagtgccacg acgcgtactc
    25801 caccagcaga ccgccgagcg ccggaccgag cagcggaccg acctgaccgg gtaacgtgac
    25861 aaaggtcatc gcagccatat attgctcgcg cggtacgatt ttcatcaccg tcaatctgcc
    25921 gaccggcacc atcatcgcgc cgccaacgcc ctgtaacgcg cgtgccagca acagttcgtt
    25981 cagcgtgccg gaaagcgcgc aaaacagtga accgagagta aacagcacga tggcggtaaa
    26041 gaaaatattg cgcacgccga ctttgtccgc cagccagccg ctggcgggca gcatcaccgc
    26101 cacggtcagc acataagaga caatgaccat gtgcatatgc aacggacttt ccccgaggct
    26161 ttgcgccatt gagggaaggg cggtgtttac gatggtggtg tccagcgact gcataaagaa
    26221 gccgaaagcc acaatccaca attgccaacg ggtgctgtcg ggaagatctg tcatttactc
    26281 ggttaccgtt tgtttaggtt tacgcgaaaa acgcagccgc agacggtcga aaaagagata
    26341 caccaccggc gtggtataca gcgtaaggag ctggctcatt accagtccgc cgacaatggt
    26401 gatccccagg ggttgccgca gctccgagcc gtcgccgccc gacaatacca gcggcagcgc
    26461 accaaacagc gccgccaggg tggtcatcat aatcgggcga aaacgcagca gacaggcctg
    26521 gaaaatagct tcctgcggcg tcaggttacc gtgccgttgg gcttcaagcg caaaatcgac
    26581 catcataatg gcgtttttct tcacgatgcc gattaatagc atgatcccta tcagggcgat
    26641 taggctgaac ggggcattga acagctccag cgccaacagc gctccaacgc ccgccgaggg
    26701 cagggtggag agaatcgtca gcggatgtac gtaactctcg taaaggatac ccagcacgat
    26761 atacaccgtg gcgatggcgg cgataatcag gatcacctgc gagttcatcg tctcctggaa
    26821 cacctgcgcc gtgccggcaa aactgccgcg caccgtcgaa ggcaccccaa gctgggtcat
    26881 tgcgcgatcg atcgccgcac tggcgtccga gagcgatttt ccggtcggca ggttaaacga
    26941 aatggtcgag gccgccgata atccctgatg attcaccgat agtggggcat tcgccggttg
    27001 ccatttagcg aaatacgaca gcgggatcgc tttgccttcg ttattgataa cgaacatttt
    27061 ttccagcgca ctgatgtcct gggtatagcg cggatccact tccatcacca ctttatactg
    27121 gttcatcggc tggtaaatgg tcgagatttg ccgctgaccg aaggcgttat ttaacagact
    27181 gttggcggct tgtacgtcga ttcccagccg tgccatggtg tcgcggtcgt aaaccagatt
    27241 catctccgcg ccgttatcct gctgatcgga gttcacgtcc gccagttccg gcaacgtcgc
    27301 cagttttttg cggattttcg gctcccattc tcgcagtgcc gccaggtcgt cggataacaa
    27361 cgtgtactgg tagctggcgt tcgactgacg cccaccaacg cgaatatcct gtaccgccat
    27421 caggaacaga ttcgcccccg gttctttcgc cagttttacg cgcagacggt cgataatttg
    27481 ctgcgccgtt tcgctgcgtt cgtcgcgtgg cttgagggtg ataaacatca tcccgctgtt
    27541 cactcgcgaa ccgcctgtaa agccggtgac attatccact gccggatcgt cacggataat
    27601 tttcatgaaa tcctgcaact taccgcgcat cgcctgaaac gaaatactct gatccgcctg
    27661 aatcccgccc atcaacacgc cagtgtcctg ctccgggaag aaggttttcg ggatcgagat
    27721 atacagccag atattcagcg caatggtgcc aagcagcacc acgcccacca gacgggtatg
    27781 attgagcacc catttcagtg acttgccgta gccttgttgc agggctacca acatgcgacc
    27841 aaaaccacgc agtcgctttt gctcgcgcgg cttgctggct ttcagcatcc agccacacat
    27901 cattggcgtt aatgtcagag aaaccagcaa cgatatacca atggcgacag aaagcgtcac
    27961 ggcaaattcg cgtaacagtc ggcccggcaa tccgcccatc aacagcagcg gcaggaacac
    28021 cgccaccagt gacagactca tcgacagcac cgtaaaaccg acttcgcgag taccttgcag
    28081 tgcggcttgc aacggtttca ttcccgcttc cagatgacgg gcaatgtttt ccagcaccac
    28141 gatggcgtca tccaccacga aaccagtagc gatggtgagc gccattaacg aaagattatt
    28201 gagactgaaa ccgcacaggt acatcgccgc aaacgtacca atcagcgaaa ccggcaccgc
    28261 aacggcggga ataatagttg cgcgacccga gcgcaggaat aaaaacacca ccagaatcac
    28321 cagcgccacc gagataatca gcgtttgctc gacttcttcc agcgaggcgc gaatggtggg
    28381 ggagcgatcc tgagcaattt gcagatcaat cgccgccgga atggtttcct gcaactccgg
    28441 taattttgcc cggatgctgt caaccgtctg gataatattg gcttccggca gcttgcggat
    28501 catcagcaaa atagccggtt tggcattggt catcccggcg ttacgtacat cctgtacaga
    28561 gtcggtcact gtcgccacat cgcccagacg aaccgcgccg ccgttgttgt aatgaataat
    28621 cagcggctga tattcggcgg cggtttttag ctcatcattg gtctggatct gccagcggtg
    28681 agtgccatct tccagcgccc cctgcggttt acgcacgttg gcattgctga tggcagtgcg
    28741 tacgtcgtcc agcgagacgc cctgattaaa cagcgcctgc ggattcagcc cgacgcgtac
    28801 ggcgggcagt gagctgccgc cgacatcgac atcaccaaca ccgtcgattt gcgaaatcgt
    28861 cggagccagc tgcgtcgagg cgaaatcgta cagtttaccc tgcgaataag tatcggatgt
    28921 cagcgtgagg atcataattg gcgcatccga cgggttagct ttgcgatagg tcggacggct
    28981 gggcatcccg ctgggcagca aactttgtgc agcgttgatc gccgcctgca catcgcgcgc
    29041 cgcgccgttg atatcccggt caaaatcaaa ctgcaaaata atacgcgtgc tgccgagcga
    29101 actgctggag gtcatttcac tgactccggc aatgcgccca agtgagcgct ccagcggcgt
    29161 ggcaacggaa gacgccattg tttctggtga cgcaccgggc agcgaggcgc tgaccataat
    29221 caccggaaaa tcgacctgcg gcagcggggc gaccggcagc atacggaagc ccagtatgcc
    29281 gcacagggta atggcaaccg acagtaaaat cgtcgccacc ggacggtaaa tgaagagggc
    29341 aaaaaacttc acttacgctt cctcttcatg gcgagcaaag cgacttttgg tccacaatgc
    29401 caggcggtcg aacagcagat aaatcaccgg cgtggtgaac agcgtcagca cctggctgac
    29461 aatcagaccg ccgaccatgc cgatacctaa cggacgacgc agttccgcgc cgaccccggt
    29521 actcaacatc agcggcagcg cgccaagcag agccgccagg gtggtcatca ggatcggacg
    29581 aaaacgcaac agacaagcct ggtagattgc atcgcgcggc gacatgcctt gctcgcgctc
    29641 agcagccagc gcgaagtcga tcatcatgat ggcgttcttc ttcacaatac cgatcagcaa
    29701 aataatgccg ataatcgcaa tcacatccag ttcgctaccg gcaatcatta atgccagcag
    29761 cgcgccgacc cctgcggtgg gtagcgtcga gagaatggtg atcgggtgaa taaagctctc
    29821 gtacagaatg ccgagcacga tatacatcgc caccaccgcc gcgacaatca gccagacagt
    29881 gctgcccagc gccgactgga aggcgagggt gctgccctgg aattgcgtgg tgatatccac
    29941 cggcagattc agcgtctttt cggtgtccat aatcgcctgc accgcatcgc ccagcgaata
    30001 gttatccggc acgttaaagg agatggtcgt taccgggaac tgatccagat ggttgatgga
    30061 aagcggcgca aaacgctgtt caactttggc aattgagctt aacggcacca cgccgccgtc
    30121 gctactggtc aggcgaatgg tatccagcgc cgcgaggccc ggggtaattt cggtgttgtg
    30181 ctccagcact acgcgatact ggttagcctg agtataaata gtggaaatca gtcgctgacc
    30241 aaacgcactg tacagggcat tatcgacatc cgccatgctg atccccagac ggctggcgct
    30301 gtcgcgatca acattgacat acgccaccag ccctttgtcc tgccagtcgc tggagacatc
    30361 agaaagctgt ggcagttgct ggagtttttc catcaactgt ggcacccagg tactgagcgc
    30421 atccagtgag gtggcctgca aggtaaactg gtactgggtg cggctgacct gagtatcaat
    30481 agtcagatcc tgcgttggtt gcaggaagag atcgacgccc ggcactttat ctaccgccgt
    30541 ttgcagacgg gcgatgactt tttgcacccg atcatcacgt tcatccaacg gtttgaggtt
    30601 gatttgtaaa cgtgcactat tcagcgacgg gttagtgcca tcaacgccaa caaatgaggt
    30661 caggctttgc actgccggat cctgcaaaat cacgtccgcg acctggcgtt gtcgctgggc
    30721 catattggca aaggagctgg attgcggtgc ctgcaaagtg ccctgaataa tgccattgtc
    30781 ctgtaccggg aagaaacctt tcggaatgaa cacccacagc agcacgctaa gcagcagcgt
    30841 gctgagtgcc acgcttaagg tcagccacgg atgattcagc actttcgcca gtccacgacc
    30901 ataggcggcg attatcctgt cgaacatttt ttccgaggca cgggagaagc ggttctgttt
    30961 acgcaacgac tcctggctga gcatccgcgc gcacatcatc ggtgtcaggg tcagcgacac
    31021 caccgctgag atcaaaatcg ctaccgccag ggtaatagca aattcgcgga acagtcgccc
    31081 gacgatatcg cccataaaca gcagtgggat caacaccgca atcagtgaga agatcagcga
    31141 gataatggta aagccgattt cacctgcgcc cttgagcgcc gccgccaacg gtttttcgcc
    31201 tttttcgata tagcgggaaa tgttttcgat caccacgatg gcgtcatcga ccacgaatcc
    31261 ggtggcgata gttaacgcca tcagtgtcag gttattgatt gaaaaatcga gaaacaccat
    31321 aaccgcgaaa gtgccgatta acgacagcgg tacagcaaca ccgggaatga tggtcgccgg
    31381 aatattgcgc aaaaacaggt agataatcat gactaccagc gcgatagcca tcatcaattc
    31441 aaactgagta tcatcgacgg atgcgcggat attggtggtg cgatcggaaa gcactgtcac
    31501 cttcaccgat ttcggcagac tctcagtgag ctgtggcagc atctgccgaa tgctgtcggc
    31561 ggtggagata atgttagcac cgggctggcg ctgaacattc atcacaatgg cctgttcttt
    31621 gttcgcccac gcgccgagcc agctgttttc tgcaccttgc tctacagttg cgacatcgcc
    31681 cagacgaatt ggcgcgccgt tctggtaggc gatgattagc tggcgatact cttcggcgga
    31741 ttgcatctgg tcgttcgcgg aaagcgtgac cgcacgggaa gggccgtcga ggctaccttt
    31801 tgccgagtta acgttagcgc cggtaatggc ggtgcgcacg gtttcgctgg tcaggccgag
    31861 ggcggcaatc gcctgagcgt taagtttgac gcgaacagcc ggacgctgac cgccggaaag
    31921 cgtcaccagg ccgacgccgg aaatctgcga gattttctgc gcgacgcggg tttccaccat
    31981 atcttccact tgcgtcatcg gcatggcggt tgaggtgacg gcgagcgtca tgatcggcgg
    32041 atctgccggg ttcactttgc tgtaaaccgg cgggttaggc agatcgctcg gcaacaagtt
    32101 ggtcgcagcg ttaatcgcgg cctgcacttc ctgctcggcg acatcgagcg gtaatgttag
    32161 ctggaactgc aaagtgataa ctgacgcacc gccggaactt tgcgacgaca tctgtttcag
    32221 gccagacatc tgcccgaact ggcgttcgag cggcgcggta acggcagagg tcatgacatc
    32281 cgggctggca cctgggtaga gcgtgaccac ctgaatggtc ggatagtcca cttccggcag
    32341 cgccgaaacg ggcagggcgc gataaccgat aatcccggcg agtaagatcg ccaccatcag
    32401 cagcgtggtg gcgacaggac gcataataaa caggcgcgac gggccgcctg tgctgctcgg
    32461 gggtaacacc tgcatcagga gcgtgctcct tttttcgcgt attcgcggct ggtggctttc
    32521 tcttccggag tggtggcgct ctgggcttcc accacttcca ctttcgcccc ttcggtcagg
    32581 cgatcaatgc cgtctgtcac cacgcgatcg cccgcagaaa tacctgcacg gatcaccact
    32641 ttctgactgt cctgaatgcc cggcgtcacc agatgtttgc tgaccttgtt ttcgctattc
    32701 agcacccaga caaaatggcc ttcattgccc atttgcaggg cggctgttgg gatcactacg
    32761 gcgttttgtt cggtgtcgac taacatgcgc gcgttaacaa actgattggg aaacagcaca
    32821 tcattctgat tattaaagcg tgctttcact ttaatcatac cggttgtggc atcgatttgg
    32881 ttatcgaggc ttaacagcgt gccttcactt aatttcttcg aattggtgcg atcccaggct
    32941 tctaccacca gcggttttcc ggctttttgc gcctgcacta cagtagcgat atcgctttcc
    33001 ggcagggtaa agactaaatc gataggatgc gtctgggtga tcaccacaat tccggtggta
    33061 tcaccactgg agatttggtt accaacatca acctgcttga gaccaacgcg accatcgact
    33121 ggtgcggtga tgcggctcca gtcgagttgc aactgcgcgc tggcgacgct tgcttcatca
    33181 gccttaatgg tgccttcggt ttcactgacc agcgcctgtt gggcatccag ctcctgacgg
    33241 gagacgagat tggtttttgc cagttgctga taacgcgcca gatcacggcg ggcgttagca
    33301 agcgtggctt tatcttttgc cagttggccc tgggtttgtg ctaatgcaac tttgaactgg
    33361 ctggggtcaa tttctgccag taaatcgcct gctttgacct gctggccttc ctggaaatgt
    33421 aacgccatca gttgaccgtc cacgcggctg cgcaccgtaa cagtattagc ggcgataatg
    33481 gtgccaagcc cggtgaggta acgcggaact gcctgttcta cggcggtcgc cgcctgaacc
    33541 ggggctaatg ggccggaacg cataccacgg cgaccacccg ctggcgattg ctgcgcttgt
    33601 ttcgtcgccc ctggggctgc actccgggag tcattgcggc cttgccagaa ccagaatgcg
    33661 gcgatggcgg cgataaccac cacgattacg attacccaac gggatttata actgcctttc
    33721 atcgttaaga gtttctcttc ctgaaacgtt tcgcggaatg atactagtgt agccagccaa
    33781 taagggagaa aaatggagga attatgaaac actgtcggga attgtctgaa tgacgggcac
    33841 atttgcgagc acgcatccag taataacaca ggaaactatt ttatctacgc gttagggata
    33901 gactgcttgc atggcgaaag gaggtaagcc gccgatttca gcgggacgct gaaacgggaa
    33961 agcccctccc gaggaagggg ccataaataa ggaaagggtc atgatgaagc tactcatcat
    34021 cgtggtgctc ttagtcatta gctggccagc ttactaagac taccagggcg ggggaaaccc
    34081 cgctctaccc tcactcctga aagtatgcct tcacgataag attgtcaatc tgcaggcttt
    34141 gcagtctgcg atcctgccag caaatattct ttgcgagacg ttacgcaata atcacactgg
    34201 aaactatttt atccgcaagt cagcgataga ctgcatacag ggcgaaagga ggtaagccga
    34261 cgatttcagc gggacgctga aacgggaaag cccctcccga ggaaggggcc ttaaataagg
    34321 aaagggtcat gatgaagcta ctcatcatcg tggtgctctt agtcataagc ttccc
//
LOCUS       DADLFA010000020        34296 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000020 DADLFA010000000
VERSION     DADLFA010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34296
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.020"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            199..258
                     /gene="ibsA"
                     /locus_tag="JQH65_000845"
     CDS             199..258
                     /gene="ibsA"
                     /locus_tag="JQH65_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HAY6138338.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            479..1138
                     /locus_tag="JQH65_000846"
     CDS             479..1138
                     /locus_tag="JQH65_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAY6138339.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            1135..1896
                     /gene="pphC"
                     /locus_tag="JQH65_000847"
     CDS             1135..1896
                     /gene="pphC"
                     /locus_tag="JQH65_000847"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HAY6138340.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVVWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAFMVQKVQDGELGLNDVLATNMVLTIRQRLFAEAEA
                     KALAVRDFACTFLGLISSPDGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTGRAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASVTQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            1893..3815
                     /gene="yegI"
                     /locus_tag="JQH65_000848"
     CDS             1893..3815
                     /gene="yegI"
                     /locus_tag="JQH65_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HAY6138341.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDIEEFVDSVAKIYHTP
                     PPASKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINVNGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDSLRQQLKKCPVSAMHVYPAHLTDCPWCTLDNQGVIY
                     FVDLGEEVITTSGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILIEIALSALSLLLCGLQAEPRYIILVPVLSAIWIIGSLTSKAYKAEIQQRREAFNRA
                     KMDYEHLVSQIQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRALAALYDTARERQKQ
                     KFLEGFFASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWKASC
                     DRRFVFRPNEAVTPADRQAVMAAKRHRLESALTVGATELQRFRLYAPARTMPLMEPLR
                     QAAEKLAQAQADLSRC"
     gene            complement(3827..5179)
                     /gene="yegD"
                     /locus_tag="JQH65_000849"
     CDS             complement(3827..5179)
                     /gene="yegD"
                     /locus_tag="JQH65_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY6138342.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEESKIALSNAAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            5313..6170
                     /gene="alkA"
                     /locus_tag="JQH65_000850"
     CDS             5313..6170
                     /gene="alkA"
                     /locus_tag="JQH65_000850"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HAY6138343.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDGTDEL"
     gene            complement(6209..9506)
                     /locus_tag="JQH65_000851"
                     /pseudo
     CDS             complement(6209..9506)
                     /locus_tag="JQH65_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            9824..10465
                     /gene="udk"
                     /locus_tag="JQH65_000852"
     CDS             9824..10465
                     /gene="udk"
                     /locus_tag="JQH65_000852"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAY6138344.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHNLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            10557..11138
                     /gene="dcd"
                     /locus_tag="JQH65_000853"
     CDS             10557..11138
                     /gene="dcd"
                     /locus_tag="JQH65_000853"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HAY6138345.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            11160..13013
                     /gene="asmA"
                     /locus_tag="JQH65_000854"
     CDS             11160..13013
                     /gene="asmA"
                     /locus_tag="JQH65_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HAY6138346.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRNYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEVVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIHGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALSGAGTLNLAEQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(13287..14870)
                     /locus_tag="JQH65_000855"
     CDS             complement(13287..14870)
                     /locus_tag="JQH65_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HAY6138347.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
     gene            15529..16668
                     /locus_tag="JQH65_000856"
     CDS             15529..16668
                     /locus_tag="JQH65_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HAY6138348.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKTYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQVLMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTFNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            16674..17117
                     /gene="wzb"
                     /locus_tag="JQH65_000857"
     CDS             16674..17117
                     /gene="wzb"
                     /locus_tag="JQH65_000857"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HAY6138349.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSWETFAAVYTLLERSARQWAQALNAEQV"
     gene            17120..19282
                     /gene="wzc"
                     /locus_tag="JQH65_000858"
                     /pseudo
     CDS             17120..19282
                     /gene="wzc"
                     /locus_tag="JQH65_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
     gene            19411..20250
                     /gene="wcaA"
                     /locus_tag="JQH65_000859"
     CDS             19411..20250
                     /gene="wcaA"
                     /locus_tag="JQH65_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HAY6138350.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTFYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            20253..20741
                     /gene="wcaB"
                     /locus_tag="JQH65_000860"
     CDS             20253..20741
                     /gene="wcaB"
                     /locus_tag="JQH65_000860"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HAY6138351.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNIWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            20738..21955
                     /gene="wcaC"
                     /locus_tag="JQH65_000861"
     CDS             20738..21955
                     /gene="wcaC"
                     /locus_tag="JQH65_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HAY6138352.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIVLFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            21930..23147
                     /gene="wcaD"
                     /locus_tag="JQH65_000862"
     CDS             21930..23147
                     /gene="wcaD"
                     /locus_tag="JQH65_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HAY6138353.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNHNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            23158..23904
                     /gene="wcaE"
                     /locus_tag="JQH65_000863"
     CDS             23158..23904
                     /gene="wcaE"
                     /locus_tag="JQH65_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HAY6138354.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQVEDISFEWIVVDGGSN
                     DGTREYLENLNGIFNLRFVSEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHQNAANF
                     VRKLKMQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQCTTSKTKALYNKV"
     gene            23920..24468
                     /gene="wcaF"
                     /locus_tag="JQH65_000864"
     CDS             23920..24468
                     /gene="wcaF"
                     /locus_tag="JQH65_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HAY6138355.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            24494..25615
                     /gene="gmd"
                     /locus_tag="JQH65_000865"
     CDS             24494..25615
                     /gene="gmd"
                     /locus_tag="JQH65_000865"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HAY6138356.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            25618..26583
                     /gene="fcl"
                     /locus_tag="JQH65_000866"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             25618..26583
                     /gene="fcl"
                     /locus_tag="JQH65_000866"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HAY6138357.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASESIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            26586..27065
                     /locus_tag="JQH65_000867"
     CDS             26586..27065
                     /locus_tag="JQH65_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HAY6138358.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDEMLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEDLLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            27062..28285
                     /gene="wcaI"
                     /locus_tag="JQH65_000868"
     CDS             27062..28285
                     /gene="wcaI"
                     /locus_tag="JQH65_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HAY6138359.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADVDV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            28288..29724
                     /gene="cpsB"
                     /locus_tag="JQH65_000869"
     CDS             28288..29724
                     /gene="cpsB"
                     /locus_tag="JQH65_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HAY6138360.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLKGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     TRHSPESDPLMLVLAADHVIADEDAFRAAVRNAIPYAEAGKLVTFGIVPDLPEIGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLPCPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            29917..31287
                     /gene="cpsG"
                     /locus_tag="JQH65_000870"
     CDS             29917..31287
                     /gene="cpsG"
                     /locus_tag="JQH65_000870"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase CpsG"
                     /protein_id="HAY6138361.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKTLGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELLCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            31342..32736
                     /gene="wcaJ"
                     /locus_tag="JQH65_000871"
     CDS             31342..32736
                     /gene="wcaJ"
                     /locus_tag="JQH65_000871"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HAY6138362.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            32738..34216
                     /gene="wzxC"
                     /locus_tag="JQH65_000872"
     CDS             32738..34216
                     /gene="wzxC"
                     /locus_tag="JQH65_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M-antigen undecaprenyl disphosphate flippase"
                     /protein_id="HAY6138363.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDLI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMIAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLVSVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYVLGIVLKGQLALGMLLG
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
BASE COUNT         8249 a         8631 c         9279 g         8137 t
ORIGIN      
        1 gtaggctttg tagtctgcga tcctgccagc aaatattctt tgcgagtcgt tacgcaataa
       61 tcacagagga aactatttta ttcacgcgtt agcgatagac tgcattcagg gcgaaaggag
      121 gtaagccgat gatttcagcg ggacgctgaa acgggaaagc ctctcccgga gaagagggct
      181 tttaataagg aaagggttat gatgaagcac gtcatcatac tggtgatact cttagtgatt
      241 agcttccagg cttactaaga acaccagggg gagggggaaa cctctcccta accctcactc
      301 ctgaaattat gtcactccgc ccttagtgtc aatcgacggc tctccgttct tgcgaggcga
      361 atagcaaaat tttctgcgcc gacgggccta attctgaaag tttcttcgtt tgctcttatt
      421 tcattattgc aatcggtatg ataccggacc tcgataattc tctaagaaag gatttcttat
      481 gagcgaacaa atcacgtttg ccaccagcga ttttgccagt aacccggaac cacgttgccc
      541 ctgcattttg ctgctggatg tttctggctc gatgagcggc agacctatta atgaacttaa
      601 cgccggattg gttacctttc gtgatgaact gcttgctgat ccgctggctt taaaaagagt
      661 ggaactcggc attgtgacgt tcggcccggt gcatgtggaa caacccttta ccagcgccgc
      721 taactttttc ccgcccatcc tgtttgccca gggggatacg cctatggggg ccgccattac
      781 caaagcccta gatatggttg aagagaggaa acgtgagtac cgcgcaaatg gtatttcgta
      841 ttatcgcccg tggattttcc tgatcaccga tggcgctcca accgatgagt ggcaggctgc
      901 cgccaacaaa gtgtttcagg gggaagaaga taagaagttt gccttctttt ccatcggtgt
      961 tcagggggcg gatatgaaga ctctggcaca aatcagcgtt cgtcagcctt tgccactgca
     1021 gggattacaa ttccgtgaac tgtttagctg gttatccagt tcgctgcgtt cggtttcccg
     1081 ctccacgccg ggaacggaag ttgtgctgga agcgccaaaa ggctggacgt cagtgtgagc
     1141 tggcgtctgg tttatgcatc cgctgttgga acatcgcaca ttagtgctga tttgccttgt
     1201 caggatgcct gccagatgca ggttgtctgg ttaaacgacc agcaaccatt actggtgatg
     1261 ttccttgctg atggcgcagg tagcgtttcg cagggaggag aaggcgcgac gctcgccgtc
     1321 aatgaagcga tggcgtttat ggtgcaaaaa gtacaggacg gtgaattggg gcttaatgat
     1381 gtcctcgcca caaatatggt actgactatt cgtcaacgac tttttgccga ggcagaagcc
     1441 aaagcattag cagttcgcga ttttgcctgc acgtttctgg gattgatttc atcgcctgac
     1501 ggcaccttga ttatgcagat tggcgatggc ggcgtggtgg tagatttggg tcacggtctg
     1561 caattgcctc tcacgccgat ggtgggtgaa tatgccaaca tgacgcactt cattaccgac
     1621 gaagatgccg tttccagact ggaaactttc accagcactg ggcgtgcgca taaagttgca
     1681 gcatttacgg atggtattca gcggctggcg ttaaatatgc tggataattc tcctcatgtg
     1741 cctttcttta ccccgttttt caatggactg gcgtcagtaa cgcaggaaca actcgattta
     1801 ttacctgaat tgttaaagca gtttttgtcc agcccggcgg taaacgagcg tacggatgat
     1861 gataaaacgt tggcactggc gctgtggtta ccatgaaacc cactttatat actgctactg
     1921 gtgagtgcgt tacgccaggc cgtgaactgg gcaaaggcgg tgaaggcgcg gtttatgata
     1981 tcgaggagtt tgtcgatagc gtcgccaaga tttatcacac gccgccaccc gcctcaaaac
     2041 aggacaaact tgcctttatg gctgcgacag ctgacgcgca gttgttgaat tatgtcgcct
     2101 ggccgcaggc aacgcttcac ggtggacgag gcggaaaagt tatcggtttt atgatgccaa
     2161 aagtttctgg taaagaaccg attcatatga tctatagccc ggcacatcgt cgccagagtt
     2221 accctcattg tgcgtgggat tttctactct atgttgcgcg caatattgct tcatcttttg
     2281 ctacggttca cgagcacggg cacgtcgtgg gtgacgtaaa ccagaacagt tttatggtag
     2341 gtcgcgacag caaagtggta ctgattgata gcgactcctt tcagattaac gtcaatggca
     2401 cactgcattt atgcgaagtc ggcgtgtcgc attttacgcc gccagagcta caaaccttgt
     2461 cgtcatttgt cggctttgaa cgcacagaaa atcacgataa ttttggcctt gcgttgctga
     2521 tttttcacgt cttgtttggt ggccggcatc cttattctgg tgtgccgctt atctctgatg
     2581 cgggtaatgc gctggagacg gatattaccc atttccgtta tgcctacgcg tcagataatc
     2641 agcgacgtgg tttaaaaccg ccgccacgat cgattccgct atcgatgtta ccgagtgatg
     2701 ttgaagccat gtttcagcag gcattcacgg aaagtggcgt ggcaaccggg cgtccgacgg
     2761 ctaaagcgtg ggtagcggca cttgattctc tgcgccaaca gttaaagaaa tgtcccgttt
     2821 cggcaatgca tgtttatccc gctcatttga ccgactgccc gtggtgtacg ctggataatc
     2881 aaggcgttat ctattttgtt gatctcggtg aagaggtcat taccaccagc ggtgattttg
     2941 tgctggcgaa agtctgggcg atggtgatgg cgtcagtagc accgccagca ttgcaattgc
     3001 cattacccga tcatttccaa ccgactggca ggccgcttcc tttaggcctg ttacggcgcg
     3061 aatacatcat tctgattgag atcgcactgt cagcgttatc gctgttgctt tgcggccttc
     3121 aggcagaacc gcgttatatt attttggttc ctgtgctgtc ggctatctgg attattggca
     3181 gtctgacaag caaagcgtac aaagcagaaa tccagcaacg ccgtgaggca tttaatcgcg
     3241 cgaaaatgga ctatgagcat ttagtcagcc agatccaaca gttgggcggg ctggaaggtt
     3301 ttatcgccaa acgggcgatg ctcgaaaaaa tgaaggacga aattctcggg ttaccggaag
     3361 aggagaaacg cgctctggca gcactttatg acaccgcaag ggaacggcag aagcagaagt
     3421 ttctggaggg attttttgcc tctattcccg gcgttggccc tgcgcgtaaa gcggcgttac
     3481 ggtcttttgg tattgaaaca gcagcggatg ttacccgtcg tggcgttaag caagttaaag
     3541 ggtttggcga ccatctgacc caggcggtta tcgactggaa agcgagctgt gaccgccgtt
     3601 ttgtgttcag gccgaacgaa gcggtaacgc cagcagacag acaggcggta atggccgcca
     3661 aacgacatcg gctggaatcg gcgttgactg tcggcgcgac agagttgcag cgattccgcc
     3721 tttatgctcc agcacggacc atgccgttga tggaaccgtt acgtcaggcg gcagaaaaac
     3781 tggctcaggc gcaggcagat ttaagccgct gctgagccat tttcttttaa cgaaatacca
     3841 cttccgccca gcgtgccagc ccggcggtga cggagccaaa gtcatcgccg cctgcaatcg
     3901 gaatgcccgg caactgttct gccagagcct ttttaatcag cggcgaacgg gcgctaccgc
     3961 cggtcaggta tataacgtcc ggtttttcct gggcgttatc cagcgccagt tgcacctgtt
     4021 ccagaatccg cgccagtggc tggctgaggg cgctttccag cccttgctgg ctaatcagcg
     4081 tagccagttc atcgctgata aacggcagtg aggcgcgggt ttccgcagcg tttgaaaggg
     4141 cgattttgct ctcttctgcg ctgcgtacca ggcgatagct taaacgctga cgccagactt
     4201 tctgtaacag ggccactttt tccggttcgc gggcatcgcg taccagatcg ttaagcagac
     4261 gaccgttggc actactgtag aaatcactct gcgcaggtac gtcgttgatg gcaaccgcat
     4321 tccaccacgg caggatcggc agggcgatgc ctttttcggt ttcgccaccc atacccagca
     4381 gtggcatcag gtttttaaac gccagggcga tatccagatc gttaccgcca atacgacaac
     4441 cactgtgacc cagcaggctg gcttcacgat cgagacgcga acgccactgc ggtcccatca
     4501 gcagcaatga acagtcagtc gtaccaccac cgatatccac caccagcacc cgtttttctt
     4561 cctgcaaggt ggcttcgtaa tccagcccag ccgcgaccgg ctcatactgg aataccacgt
     4621 ccctgaatcc ggcacgcttc gccgcgcgtt ccagaatccc ttgcgcctgg gcgtttgctt
     4681 catcgccgcc cagcccctgg aagttgatcg ggcgaccaat caccgcctga gtaatcgctt
     4741 ctggcagctg cgcctgcgcc tgctggcgaa tgtgcaacat cattgcgcag accagatcct
     4801 caaacagcgc tacctgctgc ggttttaagc cgctggcacc gaggaacgat tttggtgatt
     4861 tcacaaacca cacttcttct ggatcatcaa tgtactgtgc cagtgaggaa agaccgaact
     4921 gcacgctttt cgctgtaaca tcgatatctt cttcgcggtt ataacgaatc gcccgtcgta
     4981 acaacgcctg cgtttcatcg tcgtctgccg gaacgtcatg atggcggtac agccattcgc
     5041 ttaccgcttc acgcgttggc gcgcaaagca ttgaaggcag cagcgtgctg tcgttttcca
     5101 tttttagcaa gtgcggttta ccgtcacgca tgaccgccac tgaacagttt gctgtaccgt
     5161 aatcaaaacc aataaacacg aaataatccc catgccggtg aagaaggggc gtgactttag
     5221 cgaaatgttg ccgtcgcgac aaccggaata tgaaagcaaa gcgtagcgtc tgaataacgt
     5281 ttatgctgaa agcggatgaa taaggagatg cgatgtatac cctgaactgg cagccgccgt
     5341 atgactggtc gtggatgttg ggatttctcg ccgcccgtgc agtgagtggt gtggaaacgg
     5401 tcgctgacga ttattatgcc cgtagtctgg cggtgggcga atatcgcggc gtggtgactg
     5461 ctattccgga tatagcccgc catactctgc acataaattt aagtgcaggt ttagaacccg
     5521 ttgccgctga gtgtctggcg aaaatgagcc gcctgtttga tctgcaatgt aacccgcaga
     5581 ttgttaacgg tgcgttgggc aagttaggcg cggcgcggcc cggattgcgt ttacccggct
     5641 gtattgatgc ttttgagcag ggtgtgcggg cgattttagg ccaactggtg agcgtggcga
     5701 tggcggcaaa attgactgcc aaagttgtac agctttatgg cgaacggctg gatgattttc
     5761 cggagtatat ctgcttcccc acgcctcagc ggctggcagc agccgacccg caggcattaa
     5821 aagcgttagg catgccgttg aaacgggcag aggcactgat tcatctggca aatgcggcgc
     5881 tggagggcag cttaccaatg acaattccgg gcgatgtgga gcaggcgatg aaaacgctgc
     5941 aaacttttcc gggtatcggg cgctggacgg cgaattattt cgctttgcgt ggctggcagg
     6001 cgaaagatgt ttttctgccg gatgattatc tgattaaaca gcgatttccg gggatgacac
     6061 cggcacaaat ccgccgttat gccgagcgct ggaagccctg gcgttcttat gcgctgttgc
     6121 atatctggta tacggaaggc tggcaaccag acggaacgga cgaattgtag gcctgatacg
     6181 tcgcatcagg caatgggcaa acgctctctc agttaatcgc gaaataactg ctattcacca
     6241 gcaaatccag cggttgggca tcggcaatca catcaccata aatcagatcg acgccgatgc
     6301 cagaaagcgt atccatcact aagggtaaaa cgactggccc ggcgatggtt ttcatcccga
     6361 gtcgctgagc gtgcccctga ataatcgtaa tcagcatctc atccatcaaa ttaccctgca
     6421 cgttggcgca taactcacca tcaagtagca ggtaatctgc catattcgct ttcagcgagt
     6481 tgaagatttg cagatcgcgg cccacctggc tgagcactat ccgacatccc accagccgca
     6541 gtttttgcac gctttctgcg tgatctaaaa tcgcttcagc cggaataatc agatgtaata
     6601 accgtggtgg tagagggcta tttcccagct gctcaagcag atcattcacc agcgtggcgc
     6661 tactcaaacc ggcaacggaa agggggaggg cgatgcttat gcctttactg gcaaccgctt
     6721 ttgcggcctg ctggaaaaat tcgtggaata cccgtcggtc aagagcatgg ctaagtgccg
     6781 gatcgctgaa gctacgacga aatttttgtt catcaataat ctcgccttcg caactccaga
     6841 gcttaagtga aatcagccac aaattacgcg cttccgggat ccgcggcgaa gcgacaccgt
     6901 gggcgatcat catcaactga ttctctttaa tcatccgcca ctgttcatca agcgacatcg
     6961 ccgcccgttc gctatgtgcg gcagcttgct gtggttcgta aaccgtcacc cggccccgtc
     7021 caccattttt ggaggcataa caggcgatat cagcctgcga catcacttct gccgcctgat
     7081 gattgttgtc atcaatcaag gtaatcccgg cactggcacc tacccgatgc acacggcctt
     7141 cccatataaa gtgataatca ttcacggcac tgataatgcg tgtagcgata aaacgcgcac
     7201 tttcgatatt acagtctggc aacagtagac caaattcatc accaccgagt cgcgccagca
     7261 cgtcgctgga gcgcagcata ctcagcatca acgacgccag ttcgcgcagt aaagcgtcac
     7321 ccgccgcatg cccggcgctg tcattcaccg ctttaaagcg atcaagatcg ataaacacca
     7381 gggcatgtcg ctgatgtgta ctgtttaccg tttgcagcag gatacgcagt tgcttctcaa
     7441 aactggcgcg attggcgaga tgcgtcagtg catcatggga ggcgctgtag ctcagctggc
     7501 gcagcatttt gcgtgattcg gtgacgtcct gaatcaccag taccgagcct atattgctgc
     7561 cgtccagagt acttaacggc gtaatactgt aatgcacgtc gtaactgccg ccgttccggc
     7621 agtgcaacac cacatcttgt tcaatcgcgg aacgtgaggt gtcggcactg taaatgttct
     7681 ccattaatgg tccgttgtcg ccaaaagtaa tatgcaacac cgtcaggagc ggaacaccta
     7741 atgcttcttc ctgcgtccag ccgctcatct tctccgccac tggattcata aaggtaattt
     7801 tcatcgccat atcaatacag accacggctt caccgatgga atccagcgta atgtgcaggc
     7861 gctctttttc ctgaaacaat gcctcgttaa gctgtttaac ctcggtcata tccatattaa
     7921 tgccgagcag gcgttcgact tcgccttctt tattcagtac ccggttggcg agggcgcgga
     7981 tatggcgaat accgtctttt acggtaatgc gaaattccag cttaaagggc gagcgtgatt
     8041 gcaacgaatc acgaatcact ttttcggcat gctggcgatc ttccggcagc acgcactcgt
     8101 accacacctg ccagttcggt ttgatatgcg gaggaatttc atacagctcg aaaaatattc
     8161 ggcttcagct cccactccca gatgccaatc ccgcccgctt cgttagccag cgtgatgcgc
     8221 tccatcagtt gctgattcac ctgttcggtg cgttttagct cgttaatgtc ttcaatctga
     8281 gcgataaaat agagcggcgt gccatccgtg tggcgcacca gtgagacggc aagcaacgcc
     8341 cagacaacat cgccattacg gttgtagtag cgtttttcca ttgaataggt gtttatttca
     8401 ccgcttatca gcttttcaac ctgttggaga tctttattga gatcctccgg ccaggtcagt
     8461 tgctgaaagg tgagtccgcg cagctcttcc tgactgtacc cgagaaactg gcagagcgct
     8521 ttgttggttt gcagccattg tccctcggtg cccactaacg ccataccgat agcggaatat
     8581 tccatcgcgt tccgaaagcg ggtttcgctt tcggaaatgt gtttgcgttc cgcacgaaag
     8641 gcatacatca ccatggtcat gatgttggcg ggcagcagga tcagcaaaaa cagtagccac
     8701 ggcatatggc tcatcaggta cgtacgcggc gtagcaagca gggagggatc cgcggccatc
     8761 atcagcgaca ccatcatcac cgtggtaagg aagatcaaaa aggcttccat tcgtggcagg
     8821 cgcacggcgc tccacatcaa cagcacaata atgaaagtaa aaggccacgg ccggtacaac
     8881 atagaaagcc agcttaacgt cagcgtgatg gctaatgtga gcagtgactc aaaaagtaac
     8941 cgtgggttgc gatggcgcag cagatagtgt ggcttaaata acaatcccag cggcaccagt
     9001 accagagcgc cgatggattc tgacagtacc catatcaaaa atgccctgag aggatcgtct
     9061 ccgggcgtca gcaggacaac cagaacaccc cctaacagag gcggaacaat ggcgctgccg
     9121 aatgccagac gcagccagtc agccagattt tgcaaggggt tataccacgg cagcaattta
     9181 cgtagcagca ctgccccgac cacggcttca acaatattga tggtcgtcca ggtcatgttc
     9241 agcgagctgg tggaaaaaag caggatggat gcggcgatat ttcccagcga gcagctcagc
     9301 gcaattcccg gccacatgcg cccggcatgg cgataaaacg ccaccatcat gatggacgtc
     9361 gggaaccaca gtggggcgag ttgggtgcca aactgcgaaa gctcaagcga gaaaagggta
     9421 aagataaacg agactaaacc taaactgacc aggtgaagca gtgggtgggg cagggcaatt
     9481 aatacatgct gtgattgttt gctcattacc gctttatccg caagaccagg tcgccagtat
     9541 tcgtttctgt gtttatgcta gtacaaaagt tttacatttt atatgtgaat tgctcataaa
     9601 gtgacgtaaa ggcggataat ttgcgcaact gcgtataaca ttttttacct tacataaaac
     9661 tgatcaacgt aatttgccac cgggaattct ttgtcaacaa ttaactctga gcgcgcggca
     9721 acgctattcg actggtatca ggcggatgaa atccctataa ttgccgcgtt tggcgcttcg
     9781 tcgccccctt cctaacatcc aggttaatca ggtcgctaaa tatatgactg atcagtctca
     9841 tcagtgcgtc attatcggta tcgctggcgc atcggcttcc ggcaagagtc ttattgccag
     9901 taccctttat cgtgaattac gtgagcaagt cggtgatgaa cacatcggcg taattcccga
     9961 agactgctat tacaaagatc aaagccatct gtcgatggaa gaacgcgtta agaccaacta
    10021 cgaccatccc agcgcgatgg atcacaacct gctgcttgag catttacaag cgttgaaacg
    10081 cggctcggca attgacctgc cggtttacag ctatgttgaa catacgcgta tgaaagaaac
    10141 ggtgacggtt gagccgaaga aggtcatcat tctcgaaggc attttgttgc tgacggatgc
    10201 gcgtttgcgt gacgaactta acttctccat tttcgttgat accccgctgg atatctgcct
    10261 gatgcgccgc atcaagcgtg acgttaacga gcgtgggcgt tcaatggatt ccgtgatggc
    10321 gcaatatcaa aaaaccgtgc gcccgatgtt cctgcaattc attgagcctt ctaaacaata
    10381 tgcggacatt atcgtgccgc gcggcgggaa aaaccgcatc gcgatcgata tattgaaagc
    10441 gaaaataagt cagttctttg aataagcttg ataaattgtg taccgttcag tgataacctg
    10501 gtatgccctt gacgtaattg gcgttaaggg agtgatgcgc gaaaggagaa aatgccatgc
    10561 gtctgtgtga ccgagatatt gaagcctggc ttgatgaagg ccgtttgtcg atcaacccac
    10621 gtccgccagt ggagcgtatt aacggcgcga cggtggatgt acgcctgggc aataaatttc
    10681 gtaccttccg tggtcacacg gcagcgttta tcgatctgag cggtcccaaa gatgaagtga
    10741 gcgccgcgct tgaccgcgtg atgagcgatg agatcgttct cgacgagggc gaggcgttct
    10801 atcttcaccc aggagaactg gcgctggcgg tgacgctgga gtcggtgacg ctgccagccg
    10861 atctggtggg ctggctggac gggcgttcct ctttggcgcg tctggggctg atggtgcacg
    10921 tcaccgcgca ccgtatcgat ccgggctggt ctggttgcat tgtgctggag ttctacaact
    10981 ccggtaagct gccgctggcg ctgcgtccgg gcatgttaat tggtgcgctg agctttgagc
    11041 cgctttccgg cccggcggcg cgaccttaca accgccgtga agatgcgaaa tatcgcaacc
    11101 agcagggcgc ggtagccagc cgaatcgata aagactaatt caggcccatt gaggatacca
    11161 tgagacgatt tctgacgacg ctgatgatac tcctggtcgt gctggtggcc gggttatctg
    11221 cgttagtgtt gctggtgaat ccgaatgatt tccgcaacta tatggtcaag caagttgctg
    11281 cacgtagcgg ttatcaattg cagctcgacg ggccactgcg ttggcacgtc tggccgcagc
    11341 ttagtatcct ctccgggcga atgtctctca ccgcccaggg cgcgagccag ccactggttc
    11401 gcgccgacaa catgcgtctg gacgtggcgc ttttaccact actgagtcat caactgagcg
    11461 ttaagcaggt gatgctaaaa ggggcagtga tccaactgac gccgcagacg gaagtggtgc
    11521 gcagtgaaga cgctccggtt gcaccgcgcg acaatacctt gccggatctg tcagacgatc
    11581 gcggatggtc gtttgatata tccagtctta aggtggcgga cagcgtgctg gtgttccagc
    11641 atgaagatga cgagcaggtg acaatccgca atattcgcct gcaaatggaa caagatcccc
    11701 aacatcgtgg ttcatttgag ttctccgggc gggttaatcg cgatcagcgc gatctcacga
    11761 tatcccttaa cggtacggta gatgcttctg attatccgca tgatttaacg gcggctattg
    11821 aacaaattaa ctggcagttg cagggtgcag atttaccaaa acaaggtatt catgggcagg
    11881 ggagtttcca ggcccagtgg caggagtcac ataaacgcct ttcatttaac caaattagtt
    11941 tgaccgccaa tgacagtacg ctgagcgggc aagcacaggt cacgctgaca gagaaaccgg
    12001 aatggcagct gagactgcaa ttcccgcaac tgaatcttga caacctcatc ccgcttaatg
    12061 aaacagcgaa tggtgaaaac ggtgccgcgc agcaggggca gagccaatca acgttgccgc
    12121 gcccggtcat ttcttcgcgt attgatgaac cggcatatca gggactgcaa ggctttacgg
    12181 ctgatatttt gttgcaggcc agtaacgtgc gctggcgcgg aatgaatttt acagatgttg
    12241 ccacgcaaat gaccaacaag tcgggtttgc tggaaattac tcaactgcag ggcaagctta
    12301 acggtggaca ggtttcactg ccgggcacgc tggacgcgac atcaataaat ccgcggataa
    12361 acttccagcc acggctggaa aacgttgaga ttggcaccat tctgaaggcg tttaactatc
    12421 cgatttcgtt gaccggaaag atgtcactgg ctggtgattt ctccggtgct gacatagatg
    12481 ccgacgcatt ccgccacaac tggcaaggac aggcacatgt cgaaatgacc gacacgcgca
    12541 tggaagggat gaacttccag cagatgattc agcaagcggt agaacgtaat ggtggtgatg
    12601 tgaaggccgc tgaaaacttc gataacgtaa cgcgtcttga ccgctttacc accgatttga
    12661 cgttgaagga tggcgtcgtg acgttaaacg acatgcaagg tcaatcgcct gtgctggcgc
    12721 tttccggggc tggtacgttg aatctggcgg agcaaacctg cgacacccag tttgatattc
    12781 gggtcgtggg tggctggaac ggggaaagca aactgattga tttccttaaa gaaacgccag
    12841 taccgctgcg ggtttatggc aactggcagc aactcaatta cagtctgcaa gtggatcagt
    12901 tactgcgcaa acatctacag gacgaagcga aacgtcgtct gaatgactgg gccgagcgga
    12961 ataaagattc ccgcaatggc aaagatgtga agaagttgct ggagaagatg taggttacct
    13021 ggtgacctgt cctgtaggcc tgataagacg cgtaaagcgt cgcatcaggc gttcgtggcg
    13081 gcatcattat attctgaata tgtaagatcg aaggctgttc cgttcacttg atgttcctcg
    13141 ctactatgga acgctcaagc cggtgaacgt gccagggggg ctcaatcgcc accaccctgg
    13201 caacccgggc tcccggcggt aaatcgccgc tggcgcggtt ccctcatctt attcctttcg
    13261 gctattgggt aaaatgaccg ataccatcac acctcgtact ccaactcgcc atccttatgc
    13321 agcggaataa tctgcacctt ctgcacgcga tggctttcca cctgcaacgt tttaagcaaa
    13381 taatccccca cctgaacttc ttcgccaggt tttggaatac gctgtaaata ttccatcaac
    13441 agcccggcaa tggtgtgata ttcacgtttt tcatccagcg gcagcggcac atattgcacc
    13501 agatcttcca gcggcatatg accattcgcc gtccaggaac cgtccgcatt cttctgaata
    13561 tcatggcggg cgtcgatctc ttccacttcg ttcggtaagt taccggcaat ggtttcagtg
    13621 acgtcactta atgtcacaat cccttccacc gagccaaact catccaccac aaaagcaaag
    13681 tgtgtgcggg cattacggaa ctgctccagg gcaggtaaca acggcaaggt ttccgggaac
    13741 actagcggct ggcgaatcaa cacccgcagg ttgagcggtt cgccacgcag tgactgttgt
    13801 agcaggtcga taacgtgaac aacaccgagc aaatcttctg cgtcatcacc gtcggtaacc
    13861 accagccgcg tatgctgatt tcgctccagc aactggcgga tctcgtcttc cggcgcgttg
    13921 agatcgatat gctcaatatc gtggcgcgac gtcataatgc tgctgacagt acgctggtta
    13981 agattaagta cccgctcaat catccgccgt tcctgcggat taaagatctg ctggttacca
    14041 tgatccacca gcatggacgc ggtttcggcg tccagttccg catcttcttt ttgcccgcta
    14101 agcaggcgca ttaccgcttc ggtagtccgc tggcgtagcg tctggttagc ggaaagaaaa
    14161 cgtcgccggt taaagatagc caactgattg agcgcctcga tcattaccga gaaaccaatg
    14221 gcagcgtaca ggtagccttt cggaatgacg aagccgaaac cttctgccac caggctaaag
    14281 ccaatcatca acaggaagct taagcagaga ataacgatgg tcgggtgact gttaacaaat
    14341 tgcgttaacg gcttgctggc catcaacatc aggctgatag cgataaccac ggcggccatc
    14401 atgaccagta aatggtcgac catcccgacg gcggtaatca ctgagtcgag tgagaagatg
    14461 gcgtccagta ccacaatttg tgtcaccacc ccccagaact tcgcaccttt gcgttgtgtg
    14521 ggattattgc tgtctttccc ttccagccgt tcgttcagct ccatcgtggc tttgaacaac
    14581 aggaagaaac caccaaacag catgattaag tcgcgggcgc taaaggtaaa cgagcggaag
    14641 ctgaacagcg gttgcgtcag ggtgaccagc caggagattg acgccagcag taacaggcgc
    14701 attaacatcg ccagcaacag cccggtaacc cgtgcgcggt cgcgctgctt cggcggtagt
    14761 ttttcggcga ggatggcaat aaagaccagg ttatcaatgc cgaggaccag ttcgatcaca
    14821 atcagcgtga ttaacccggc ccagatagac ggatcggcaa tccattccat agtaagtaca
    14881 aaacctattc gttgtatgac aaatgtcaca attcgatcat ggataaatcg gggcaaaaat
    14941 gcaatgttgc agcactattt atctcatatt atttgagggt aataacctca cattatccct
    15001 gaattaaaag tggtaataat aaaacattat ttaagaaata tcgcatgcgg aggcgcaata
    15061 ttaaatattg tcaacctaaa gaaactccta aaaaccatat tgaatgacac ttaatataat
    15121 tcttaaaaat agccaattgc cgaattgtta ccttgcctgc tattccgtta gctgtaacac
    15181 ttcctcctgc attattggag agccaatatt caattaccat aatgtcacct tatattcgga
    15241 agttaatttc cgcaataacc ctttctcatc aacgattgta tgcagagagg gtttaaaaaa
    15301 ttaagctaaa tcagtgtatt ggtggctaaa aagccagggg cggtagcgtg tctggatgcc
    15361 tgaaagacca gtctgaatta ttctgtcaat agcctgcgga tgaaatcaat ttttttagga
    15421 ctgatgccag ttaaatttgt attcaattaa ctgcaaggta aatgtgcatg gcgacattgc
    15481 tattaatagt gcacaggata attactctgc caaagtgata aataaacaat gatgaaatcc
    15541 aaaatgaaat tgatgccatt attggtgtca gtaaccttga taagcggttg cacagtactt
    15601 ccgggcagca atatgtcgac gatgggcaaa gacgtcatca aacagcagga cgctgatttc
    15661 gatctcgaca aaatggtgaa tgtttatccg ctgaccccgc gcctgattga ccaattacgc
    15721 ccacgtccga atgtagcgcg ccccaatatg acgctggaaa gcgagatcgc gaattaccag
    15781 tatcgcgtcg ggccggggga cgttcttaat gtcaccgtct gggatcaccc ggaactcacc
    15841 acgccagctg gtcagtaccg cagctccagc gacaccggca actgggtaca gcctgacggc
    15901 actatgtttt acccgtatat cggcaaggtt cacgtagtcg ggaaaacgct cgctgaaatc
    15961 cgcagtgata ttaccgggcg cttagcgacg tacatcgctg acccgcaggt ggacgttaat
    16021 atcgccgcct tccgctcaca aaagacctat atctcaggtc aggtgaataa atccggtcaa
    16081 caggcgatca ccaacgtacc actgaccatt ctcgacgcca tcaacgccgc aggtggcctg
    16141 accgacaccg ctgactggcg caacgtggtg ctaacacaca atggtcgtga agaacgcatt
    16201 tctttgcagg tgctgatgca aaacggcgac ctcaatcaga accgactgct ttatcccggc
    16261 gatattctct acgtgccgcg taatgatgat ctgaaagtat ttgtgatggg tgaagtgaag
    16321 aaacagagca ccctgaaaat ggactttagc ggcatgaccc tgactgaagc cctgggtaat
    16381 gctgaaggta tcgacatgac caccttcaac gccagcggca tctttgtcat tcgtccgctg
    16441 aaaggcgagg gcgggcgtaa cggcaagatt gccaatatct accagctgga tatgtccgat
    16501 gccacgtcgc tggtgatggc gacagaattc cgcctgcaac cttatgacgt ggtgtatgtc
    16561 accaccgccc cggtttcccg ctggaaccgt ctgatcaatc agttgctgcc aactattagc
    16621 ggtgtccgtt acatgacgga tacagccagc gacattcata actggtaatc gtcatgttta
    16681 acaacatatt agttgtctgt gtcggcaata tttgccgttc cccgacggcg gaacgcttac
    16741 tgcaacgtta tcacccggaa ctgaaagtgg agtccgctgg actcggcgcg ctggtcggta
    16801 agggcgctga tcctaccgct atcagcgtcg ccgcagaaca tcaactgtct ctggaaggcc
    16861 actgtgcacg acaaatcagc cgccgcctgt gtcgcaacta tgacctgatt ttgaccatgg
    16921 aaaagcgcca tatcgaacgc ttatgcgaga tggcacctga gatgcgcggc aaagtgatgc
    16981 tgtttggtca ctgggataac gaatgtgaaa tccccgatcc gtatcgcaaa agctgggaaa
    17041 cgtttgcagc ggtgtacaca ttacttgaac ggtctgcccg ccagtgggcg caggcattga
    17101 acgcagagca ggtataagaa tgacagaaaa agtaaaacaa catgccgctc cggtaacggg
    17161 cagtgatgaa atcgatattg gtcgcctggt cggcaccgtc attgaagcgc gctggtgggt
    17221 gattggcatc accgctgtat ttgtcctctg tgccgtggtt tacaccttct tcgccacgcc
    17281 gatttatagt gccgacgcac tggtacaaat cgagcaaaac agcggcaatt cgttagtgca
    17341 ggacattggt tcggcattag ccaacaaacc gcctgcatcg gacgccgaga tccagttgat
    17401 tcgttcgcgt ctggtgcttg gtaaaacggt ggacgatctc gacctcgata ttgcagtgag
    17461 caaaaacacg ttcccgattt tcggggcggg ctgggatcga ctgatgggac gccagaacga
    17521 gacggtgaaa gtgactacct ttaaccgccc gaaagagatg gcggactagg tgtttacgct
    17581 taatgtgctg gacaataaaa actacaccct gagcagtgat ggcggcttta gcgcccgtgg
    17641 gcaagcgggc cagatgctga aaaaagaagg cgtcacgctg atggttgaag ccattcacgc
    17701 ccgcccgggc agtgagttta ccgtcaccaa atactccacg ttggggatga tcaaccaact
    17761 gcaaaacagc ctgatggtaa cggagaacgg caaagacgca ggcgtactga gcctgactta
    17821 taccggtgaa gatcgcgaac agatccgcga cattcttaac agcatcgccc gtaactatca
    17881 ggaacaaaat attgagcgca aatcggcgga agcgtcgaaa agcctcgctt tcctcgcgca
    17941 acagttaccg gaagtacgta gccgcctcga tgttgccgaa aacaaactga atgccttccg
    18001 tcaggataaa gattctgttg atctgccgct ggaagcgaaa gcggtgctcg attcgatggt
    18061 gaacatcgac gcgcagttga acgaactgac ctttaaagag gcggaaatct ccaagctgta
    18121 caccaaagtt cacccggcgt accgcacgct gctggagaaa cgtcaggcgc tggaagaaga
    18181 gaaagccaaa cttaacggtc gcgtaacggc gatgccgaaa acccagcagg agattgtgcg
    18241 cctgacccgc gatgtcgagt ctggtcagca ggtctatatg caactgctga ataaagagca
    18301 ggagctgaaa atcaccgaag ccagcaccgt cggcgatgtg cgcattgttg acccggcaat
    18361 cacccagcct ggcgtgctaa aaccgaagaa agggctgatt atcctcggcg cgattatcct
    18421 tggcctgatg ctttctatcg tgggtgtgct gctgcgctcg ttgtttaatc gcggcattga
    18481 aagcccgcaa gtactggaag aacacggtat tagcgtctat gccagcatcc cgctgtcgga
    18541 atggcagaaa gcgcgtgata gcgtcaaaac catcaaaggg attaaacgct ataaacagag
    18601 ccagctactg gcggtgggga atccaaccga tctggcgatt gaagccatcc gtagtctgcg
    18661 taccagtttg cacttcgcga tgatgcaggc acagaacaat gtgttgatga tgaccggggt
    18721 tagcccgtca attggtaaaa cctttgtctg cgccaacctg gcggcggtga tcagccagac
    18781 caataaacgc gtgttgttga tcgactgcga tatgcgcaaa ggctacaccc acgagctgtt
    18841 gggcaccaat aacgtcaatg gcctgtcgga aattctgatt ggtcagggcg atattactac
    18901 cgcagctaaa ccgacctcta ttgccaaatt tgacctgatc ccgcgcggtc aggtgccgcc
    18961 aaatccttct gaactgttga tgagcgaacg ctttgccgaa ctggtgaact gggcgagtaa
    19021 aaactatgac ctggtgttga ttgatacgcc gccgattctg gcagtgaccg atgcggcaat
    19081 tgttggtcgt catgtcggaa ccacattaat ggtggcgcgt tatgcggtca acacattgaa
    19141 agaagtggaa accagtctga gccgctttga gcaaaacggt attccggtga aaggggtgat
    19201 tctgaactcc atcttccgcc gcgccagcgc gtatcaggat tatggctatt acgaatacga
    19261 atataagtcg gatgcgaaat aacgagatcc gttgtcggat gcgatgctga cgcatcttat
    19321 ccgacctacg ttgacgccgc atccggcaac agaacccgta aacaagctta taaaccggca
    19381 gaacatctcg acactccggg agaacaacct atgaaagaca atccgctgat ctcaatctat
    19441 atgccgacct ggaaccgcca acaactggcg attcgggcga taaaatcggt cttgcgccag
    19501 gactacagca actgggagat gatcatcgtg gatgattgct ccacctcctg ggaacaactg
    19561 caacagtacg tcaccgccct caacgatccg cgtatcactt acattcacaa cgacattaac
    19621 agcggggcgt gcgcggtacg taaccaggct attatgctgg cgcaagggga atacattacc
    19681 gggatcgatg atgatgatga atggacaccc aaccgtctga gcgtcttcct cgcccataaa
    19741 cagcaactgg tcactcacgc ctttttgtac gctaacgact acgtttgcca gggagaagtt
    19801 tattcccaac cggcaagctt accactgtat cccaaatcgc cgtactcacg ccgcctgttc
    19861 tataaacgca atatcattgg caatcaggtc tttacctggg catggcgttt caaagagtgt
    19921 ttgttcgata ccgaactgaa agcggcacag gattacgaca tcttcctgcg gatggtggtg
    19981 gagtacggcg aaccgtggaa agtggaagag gcgacgcaga tcctgcatat caatcacggt
    20041 gaaatgcaga tcacctcgtc accgaaaaaa ttctccggat acttccactt ttaccgcaag
    20101 cacaaagaca aatttgaccg cgccagcaaa aaatatcagc tgtttacctt ctaccagatc
    20161 cgcaataaac gtatgacctg gcgcactttg ctgacgctgc tgtcggtgcg taacggcaaa
    20221 cgtctggctg atggtattcg ggggcgctaa agatgctgga agatctgcgc gccaacagct
    20281 ggagtttacg tccgtgctgc atggttctag cgtatcgcgt cgctcatttt tgttcggtgt
    20341 ggcgcaaaaa gaacgtcctc aacaatattt gggcggcccc tctgctggtg ctgtatcgca
    20401 ttatcaccga atgctttttc ggttatgaaa tccaggctgc cgcgaccatt ggccgccgct
    20461 ttactatcca tcacggttac gccgtagtga tcaataaaaa cgtggtggcg ggggatgatt
    20521 tcaccattcg ccacggcgtc actatcggca atcgtggtgc cgataacatg gcatgtccac
    20581 atattggcaa cggcgtcgaa ctcggtgcca acgttattat tcttggtgat atcacgcttg
    20641 gtaacaacgt caccgtgggt gcgggcagcg tggtgctcga ctctgtcccg gacaacgcgc
    20701 tggtggtggg agaaaaagcg cgagtgaagg taattaaatg aatattttgc aatttaatgt
    20761 gcgactggcg gaaggcgggg cagcaggtgt ggcgttagat ctccaccagc gtgcgctgca
    20821 acaggggctt gcgtcacatt ttgtctacgg ttacggcaaa ggcggcaaag agagcgtcag
    20881 ccatcaaaac tatccgcagg tcatcaaaca tacgccgcgg atgactgcaa tggcgaatat
    20941 tgtcctgttt cgtctgttta atcgcgatct gtttggcaat ttcaatgagt tatatcgcac
    21001 cattactcgt acaccgggtc cggtggtcct gcattttcat gtgctgcaca gctactggct
    21061 aaatcttaag agcgtggtgc gcttttgcga aaaggtgaaa aaccataaac cggacgtcac
    21121 tctggtctgg acgctgcacg accactggag cgttaccgga cgctgcgcct ttaccgacgg
    21181 ttgtgaaggc tggaaaacgg gctgccagaa atgcccgacc ttaaataact atccgccggt
    21241 gaagattgat cgggcgcacc agctggtggc gggcaaacgc cagttattcc gtgagatgct
    21301 ggcgctgggc tgtcagttta tttcccccag ccagcatgtg gctgacgctt tcaatagcct
    21361 gtacggtcca gggcgttgcc ggattatcaa taatggtatt gatatggcaa ccgaagcgat
    21421 tctggcggat ctgcctccgg tgcgcgaaac ccagggcaag ccgaaaatcg ctgtggtggc
    21481 gcatgacctg cgttacgacg gcaaaactaa ccagcaactg gtgcgtgaga tgatggcgct
    21541 gggcgacaaa atcgaactgc atacctttgg taagttctcg ccgttcaccg ctggcaacgt
    21601 ggttaatcac ggctttgaaa ccgacaaacg taagctgatg agcgcgctca atcagatgga
    21661 tgcgctggta ttcagttctc gcgtcgataa ctacccgctg attttgtgtg aggcgctatc
    21721 gattggcgtg ccggtgattg ccacccatag cgatgcggcg cgggaagtgt tgcaaaaatc
    21781 cggcggtaaa accgtcagcg aagaagaggt gctgcaactg gtgcagttaa gcaaaccgga
    21841 aatcgcgcag gcgatatttg gtaccacgct ggctgagttc agccaacgca gccgcgccgc
    21901 ctacagtgga caacagatgc tggaggagta tgtcaacttc tatcagaatc tgtagctacc
    21961 tgttgctgcc gctgatttat ctgctggtta acgtcaaaat cgcccagctt ggcgaaagtt
    22021 tccccatcac catcgtcact tttttacctg tcttgttact gctgttttta gaacgcatca
    22081 gcgttaaaaa attgatgatt gccttaggga ttggcgcggg actcacggcg tttaactatc
    22141 tgtttggtca gtcgctggat gccagtaaat acgtcacttc aactatgctg tttgtctata
    22201 ttgtgatcat tattggcatg gtgtggagta ttcgttttaa aacaatttcg ccacacaacc
    22261 atcgcaagat attacgtttc ttttatctgg tggtcgggct ggtggtggcg ctggcggcgg
    22321 tggagatggc acaaattatc ctcaccggtg gcagcagtat tatggagtcg atttcgaaat
    22381 atctgattta cagcaacagc tatgtgctga atttcattaa attcggcggc aagcgcacga
    22441 cagcacttta tttcgaaccg gcatttttcg ctctggcatt aatctcaatt tggctcagca
    22501 tcaaacagtt tggtatcaaa acgcctaaaa cagatgctat gattctcgca gggataatat
    22561 tatccggatc gttttcaggg gttatgacct ttatcctgtt ttatttgctg gagtgggcat
    22621 ttcaatatct gaataaagag gcgattaaga aaaagttacc gttagcattg atttctctgg
    22681 ctgtattcct ggttggtgtg gtaatcgcgt ttccttatat ttccacccgt ctgggcgatt
    22741 taggtacgga aggatcgtca tcatattatc gtattgtcgg tccgctggtg atggtcggtt
    22801 attctttgac ccatattgac ggtgtagtca gatttggctc actttatgaa tatgtcgcat
    22861 cattcggaat atttaacggt gcggatgtcg gaaaaaccat agacaatggt ttgtatctgc
    22921 tgattattta tttttcctgg ttcgcggtgt ttttatcact gtggtacatg gggaaagtga
    22981 taaaaatgat gatcaacgct tttggtgata accacaattt tcgcgtgcaa ttatatcttt
    23041 ttactccggt atcgctgttt tttaccggtt cgatatttag cccggaatat gcatttttaa
    23101 tcgtctgtcc gtttattttg cgaaaagcgt taaatattac gaggtaagaa taagaacatg
    23161 ttgcttagca taatcactgt cgcgtttcgt aacctcgaag ggatagtcaa aacacatgcc
    23221 tcgctggcgc atctggcgca ggtggaagat atcagcttcg aatggattgt tgtcgatggc
    23281 ggttccaacg acggcacccg tgagtatctg gaaaatctca atggtatctt taacctacgc
    23341 tttgtcagcg agccagataa cggtatctac gacgccatga acaaaggcat tgcgatggca
    23401 caaggcaagt tcgcgttgtt tctcaattcg ggcgatattt ttcatcagaa tgccgcaaat
    23461 tttgtccgta agttaaaaat gcaaaaagat aacgtgatga tcaccggcga tgcgctgctg
    23521 gattttggcg acgggcataa aattaaacgt agcgccaaac cgggctggta tatttatcac
    23581 agcctgcccg ccagtcatca ggcgatattt ttccctgtat ccggcttgaa aaaatggcgt
    23641 tatgacctgg aatataaagt ttcttccgac tacgcgctgg cagccaaaat gtataaagcc
    23701 ggttatgcat ttaaaaaact caatggcctg gtgtctgaat tttccatggg tggggtatct
    23761 accaccaata atatggaatt gtgtgctgac gcgaaaaaag tccaacgaca aatattacat
    23821 gtgcctggct tttgggctga attatcctgg catttacgcc aatgtactac ctcaaagacg
    23881 aaagccttat ataacaaagt ctgaatataa ggaaaaccaa tgcaagattt aagcggtttc
    23941 tcggtgccga aagggttccg gggcggcaac gctattaaag tgcaattatg gtgggcagta
    24001 caggcaacaa tatttgcctg gtcgccacaa gtattgtatc gctggcgggc ttttttatta
    24061 cgtttattcg gagcaaaaat aggaaaaaac gtagttattc gtccgtcagt aaaaattacc
    24121 tatccgtgga aattaacctt aggtgattac gcgtgggtcg gcgatgacgt caatttatat
    24181 accctcggtg aaataaccat tggcgcacat tcggtgatat cgcaaaaaag ttatttatgc
    24241 accggtagcc acgaccatgc aagtcaacat ttcaccatta acgccacgcc tattgtgatt
    24301 ggcgagaaat gctggctggc aaccgatgtc tttgttgccc caggcgtcac aatcggcgac
    24361 ggcaccgtcg tgggtgcacg aagcagtgtt tttaaatcgc ttccggcaaa tgtggtttgc
    24421 cgggggaatc ccgcagtggt gatacgcgaa cgcgttgaaa ctgaataaat tcaaaaatac
    24481 agaggaataa tacatgtcaa aagtcgctct catcaccggt gtaaccggac aagacggttc
    24541 ttacctggca gagtttctgc tggaaaaagg ttacgaggtg catggtatta agcgtcgcgc
    24601 atcgtcattc aacaccgagc gcgtggatca catttatcag gatccgcaca cctgcaaccc
    24661 gaaattccat ctgcattatg gcgacctgag tgatacctct aacctgacgc gcattttgcg
    24721 tgaagtacag ccggatgaag tgtacaacct gggcgcaatg agccacgttg cggtctcttt
    24781 tgagtcacca gaatataccg ctgacgtcga cgcgatgggt acgctgcgcc tgctggaggc
    24841 gatccgcttc ctcggtctgg aaaagaaaac tcgtttctat caggcttcca cctctgaact
    24901 gtatggtctg gtgcaggaaa ttccgcagaa agagaccacg ccgttctacc cgcgatctcc
    24961 gtatgcggtc gccaaactgt acgcctactg gatcaccgtt aactaccgtg aatcctacgg
    25021 catgtacgcc tgtaacggaa ttctcttcaa ccatgaatcc ccgcgccgcg gcgaaacctt
    25081 cgttacccgc aaaatcaccc gcgcaatcgc caacatcgcc caggggctgg agtcgtgcct
    25141 gtacctcggc aatatggatt ccctgcgtga ctggggccac gccaaagact acgtaaaaat
    25201 gcagtggatg atgctgcagc aggaacagcc ggaagatttc gttatcgcga ccggcgttca
    25261 gtactccgtg cgtcagttcg tggaaatggc ggcagcacag ctgggcatca aactgcgctt
    25321 tgaaggcacg ggtgttgaag agaagggcat tgtggtttcc gtcaccgggc atgacgcgcc
    25381 gggcgttaaa ccgggtgatg tgattatcgc cgttgacccg cgttacttcc gtccggcaga
    25441 agttgaaacg ctgctcggcg acccgaccaa agcgcacgaa aaactgggct ggaaaccgga
    25501 aatcaccctc agagagatgg tgtctgaaat ggtggctaat gacctcgaag cggcgaaaaa
    25561 acactctctg ctgaaatctc acggctacga cgtggcgatc gcgctggagt cataagcatg
    25621 agtaaacaac gcatttttat cgctggccat cgcgggatgg tcggttctgc catcaggcgg
    25681 cagctcgaac agcgcggtga tgtggaactg gtattacgca cccgcgacga gctgaacctg
    25741 ctggacagcc gcgctgtgca tgatttcttt gccagcgaaa gcattgacca ggtctatctg
    25801 gcggcggcga aagtgggcgg cattgttgcc aacaacacgt atccggcgga tttcatctac
    25861 cagaacatga tgattgagag caacatcatt cacgccgcgc atcagaacga cgtgaacaaa
    25921 ctgctgtttc tcggatcgtc ctgtatctac ccgaaactgg caaaacagcc gatggcagaa
    25981 agcgagttat tgcagggtac gctggagccg accaacgagc cttacgccat tgccaaaatc
    26041 gccgggatca aactgtgcga atcttacaac cgccagtatg gacgcgatta ccgctcggtc
    26101 atgccgacca acctgtacgg gccacacgac aacttccatc cgagtaactc gcatgtgatc
    26161 ccagcattgc tgcgtcgctt ccacgaggcg acggcacaga atgcgccgga cgtggtggtg
    26221 tggggcagcg gtacaccgat gcgtgaattt ctgcacgtcg atgatatggc ggcggcgagc
    26281 attcatgtga tggagctggc gcacgaagtc tggctggaaa acacccagcc gatgctgtca
    26341 cacattaacg tcggcacggg cgttgactgc actatccgcg agctggcgca aaccatcgcc
    26401 aaagtggtgg gttacaaagg tcgggtggtt tttgatgcta gcaagccgga tggtacgccg
    26461 cgcaaactgc tggatgtgac gcgcctgcat cagcttggct ggtatcacga aatctcactg
    26521 gaagcggggc ttgccagcac ttaccagtgg ttccttgaga atcaagaccg ctttcggggg
    26581 taatgatgtt tttacgtcag gaagactttg ccacggtagt gcgctccact ccgcttgtct
    26641 ctctcgattt tattgtcgag aacagtcgcg gcgagtttct gcttggcaaa agaaccaacc
    26701 gcccggcgca gggttactgg tttgtgccgg gagggcgcgt gcagaaagac gaaatgctgg
    26761 aagccgcatt tgagcggctg acgatggcgg aactggggct gcgtctaccg ataacagcag
    26821 gccagtttta cggtgtctgg cagcactttt atgacgataa cttctccggc acggatttca
    26881 ccactcacta tgtggtgctc ggttttcgct tcagagtcgc tgaagaagat ctgttactgc
    26941 cggatgagca gcatgacgat taccgctggc tgacgccgga cgcgctgctc gccagtaacg
    27001 atgttcacgc taacagccgc gcctattttc tcgctgagaa gcgtgccgga gtacccggat
    27061 tatgaaaata ctggtctacg gcattaacta ctcgccggag ttaaccggca tcggcaaata
    27121 caccggagag atggttgaat ggctggcggc gcaaggtcat gaggtgcggg ttattaccgc
    27181 accgccttac tacccgcagt ggcaggtggg cgagaactat tccgcctggc ggtacaaacg
    27241 agaagagggg gccgctacgg tgtggcgctg cccgctgtac gtgccaaaac agccgagcac
    27301 cctgaaacgc ctgttgcatc tcggcagttt tgccgtcagc agttttttcc cgttgatggc
    27361 gcaacgtcgc tggaagccgg atcgcattat cggcgtagtg ccaacgctgt tttgcacgcc
    27421 gggaatgcgc ctgctggcga aactctctgg cgcgcgtacc gtgctgcata ttcaggatta
    27481 cgaagtggac gccatgctgg ggctgggcct tgccggaaaa ggcaaaggcg gcaaagtggc
    27541 acagctggca acggcgttcg aacgtagcgg actgcataac gtcgataacg tctccacgat
    27601 ttcgcgttcg atgatgaata aagccatcga aaaaggcgtg gcggcagaaa acgtcatctt
    27661 cttccccaac tggtcagaaa ttgcccgttt tcagcatgtc gcagatgtcg atgttgatgc
    27721 ccttcgtaac cagcttggcc tgccggataa caaaaaaatc attctttact ccggcaatat
    27781 tggtgaaaag caggggctgg aaaacgttat tgaagctgcc gatcgcctgc gtgatgaacc
    27841 gctgattttt gccattgtcg ggcagggcgg cggcaaagcg cggctggaaa aaatggcgca
    27901 acagcgtggt ctgcgcaaca tgcaattttt tccgctgcaa tcgtatgacg ctttacccgc
    27961 actgctgaag atgggcgatt gccatctggt ggtgcaaaaa cgcggcgcgg cagatgccgt
    28021 attgccgtcg aaactgacca atattctggc ggtaggcggt aacgcggtga ttactgcaga
    28081 agcccacaca gaactgggac agctttgcga aacctttccg ggcattgcgg tttgcgttga
    28141 accggaatcg gtcgaggcgc tggtggcggg gattcgtcag gcgctcctgc tgcccaaaca
    28201 caacacggtg gcacgtgaat atgccgaacg cacgctcgat aaagagaacg tgttacgtca
    28261 atttataaat gatattcggg gataattatg gcgcagtcga aactctatcc agttgtgatg
    28321 gcaggtggct ccggtagccg cttatggccg ctttcccgcg tactttaccc caagcagttt
    28381 ttatgcctga aaggcgatct caccatgctg caaaccacca tctgccgcct gaaaggcgta
    28441 gagtgcgaaa gcccggtggt gatttgcaat gagcagcacc gctttattgt cgcggaacag
    28501 ctgcgccaac tgaacaaact caccgagaac attattctcg aaccggcagg gcgtaacact
    28561 gcaccagcca ttgcgctggc ggcgctggcg gcaacacgtc atagtccgga gagcgacccg
    28621 ttaatgctgg tgctggcggc ggatcatgtg attgccgatg aagacgcgtt ccgtgccgcc
    28681 gtgcgtaatg ccataccgta tgccgaagcg ggcaagctgg tgacctttgg cattgtgccg
    28741 gatctacctg aaatcggtta tggctatatt cgtcgcggtg aagtgtctgc gggtgagcag
    28801 gatacggtgg cctttgaagt ggcgcagttt gtcgaaaaac cgaatctgga aaccgctcag
    28861 gcctatgtgg caagtggcga atattactgg aacagcggca tgttcctgtt ccgcgccgga
    28921 cgctatctcg aagaactgaa aaaatatcgc ccggatattc tcgatgcctg tgaaaaagcg
    28981 atgagcgccg tcgatccgga tctcgatttt attcgcgtgg atgaagaagc gtttctcccc
    29041 tgcccggaag agtcggtgga ttacgcggtc atggaacgta cggcagatgc tgttgtggtg
    29101 ccgatggatg cgggctggag cgatgtcggt tcttggtctt cattatggga gatcagcgcc
    29161 cacaccgccg agggcaatgt ttgccacggc gatgtgatta atcacaaaac tgaaaacagc
    29221 tatgtgtatg ccgaatctgg cctggtcacc accgtcgggg tgaaagattt ggtggtagtg
    29281 cagaccaaag atgcagtgct gattgccgac cgtaacgcgg tgcaggatgt gaaaaaagtg
    29341 gtcgagcaga tcaaagccga tggtcgccat gagcatcggg tgcatcgcga agtgtatcgt
    29401 ccgtggggca aatatgactc tatcgacgcg ggcgaccgct accaggtgaa acgcatcacc
    29461 gtgaaaccgg gcgaaggctt gtcggtacag atgcatcatc accgcgcgga acactgggtg
    29521 gttgtcgcag gaacggcaaa agtcactatc gacggtgata tcaaactgct tggtgaaaac
    29581 gagtccattt atattccgct gggggcgacg cattgcctgg aaaacccggg gaaaattccg
    29641 ctcgatttaa ttgaagtgcg ctccggctct tatctcgaag aggatgatgt ggtgcgcttc
    29701 gcggatcgct acggacgggt gtaaacgtcg catcaggcgc tgtttgtcgg atgcggcgtg
    29761 aacgccttat ccgacctacg gttcggattt gtaggcctga taagacgcgg cagcgtcgca
    29821 tcaggcaatg aatgcgaaac cgagatgtaa ataacgacaa aaacaaaatt ggccgtttcg
    29881 gtcagggcca actattgcct gaaaaagggt aacgacatga aaaaattaac ctgctttaaa
    29941 gcctatgata ttcgcgggaa attaggcgaa gaactgaatg aagatatcgc ctggcgcatt
    30001 ggtcgcgcct atggcgaatt tctcaaaccg aaaaccattg tgttaggcgg tgatgtccgt
    30061 ctcaccagcg aaaccttaaa actggcgctg gcgaaaggtt tacaggatgc gggcgtcgat
    30121 gtgctggata ttggcatgtc cggcaccgaa gagatctatt tcgccacgtt ccatctcggc
    30181 gtggatggcg gcattgaagt taccgccagc cataatccga tggattataa cggcatgaaa
    30241 ctggtgcgcg aaggggctcg cccaatcagc ggtgataccg gactgcgcga cgtccagcgt
    30301 ctggcagaag ccaacgactt tcctcccgtc gatgaaacca aacgcggtcg ctatcagcaa
    30361 atcaacctgc gtgacgctta cgttgatcac ctgttcggtt atatcaatgt caaaaacctc
    30421 acgccgctca agctggtgat taactccggg aatggcgcag cgggtccggt ggtggacgcc
    30481 attgaagccc gctttaaaac cctcggcgcg cccgtggaat tgatcaaagt gcacaacacg
    30541 cccgacggca atttccccaa cggtattcct aacccgctgc tgcctgaatg ccgcgacgac
    30601 acccgcaatg cggtcatcaa acacggcgcg gatatgggca ttgcctttga tggcgatttt
    30661 gaccgctgtt ttctgtttga cgaaaaaggg cagtttatcg agggctacta cattgtcggc
    30721 ctgctggcag aagcgttcct cgaaaaaaat cccggcgcga agatcatcca tgatccgcgt
    30781 ctctcctgga acactattga tgtggtgact gccgcaggcg gcaccccggt aatgtcgaaa
    30841 accggacacg cctttattaa agaacggatg cgcaaggaag acgccatcta cggtggcgaa
    30901 atgagcgctc accattactt ccgtgatttc gcttactgcg acagcggcat gatcccgtgg
    30961 ctgctggtcg ccgaactgtt gtgcctgaaa ggaaaaacgc tgggcgaact ggtgcgcgac
    31021 cggatggcgg cgtttccggc aagcggtgag atcaacagca aactggcgca acccgttgag
    31081 gcgattaatc gcgtcgaaca gcattttagc cgtgaggcgc tggcggtgga tcgtaccgat
    31141 ggcatcagca tgacctttgc cgactggcgc tttaacctgc gcacctccaa caccgaaccg
    31201 gtggtgcggt tgaatgtgga atcgcgcggt gatgtgccgc tgatggaagc gcgaacgcga
    31261 actctgctga cgttgctgaa tgagtaatgt cggatcttcc cttaccccac tgcgggtaag
    31321 gggctaataa caggaacaac gatgacaaat ctaaaaaagc gcgagcgagc gaaaaccaat
    31381 gcatcgttaa tctctatggt gcaacgcttt tcagatatca ccatcatgtt tgccggacta
    31441 tggctggttt gcgaagtgag cggactgtca ttcctctaca tgcacctgtt ggtggcgttg
    31501 attacgctgg tggtgttcca gatgctgggc ggcatcaccg atttttatcg ctcatggcgc
    31561 ggtgttcggg cagcgacaga atttgccctg ttgctacaaa actggacctt aagcgtgatt
    31621 ttcagcgccg gactggtggc gttcaataat gatttcgaca cgcaactgaa aatctggctg
    31681 gcgtggtatg ggctgaccag tatcggactg gtggtttgcc gttcatgtat tcgcattggg
    31741 gcgggctggc tgcgtaatca tggctataac aagcgtatgg tcgccgtagc gggagattta
    31801 gccgccggac aaatgctgat ggagagcttc cgtaatcagc cgtggttagg gtttgaagtg
    31861 gtgggcgttt accacgaccc gaaaccgggt ggcgtttcta acgactgggc gggcaatctg
    31921 caacagctgg tcgaggatgc gaaagcgggc aagattcata acgtctatat cgcgatgcaa
    31981 atgtgcgacg gcgcacgagt gaaaaaactg gtccatcaac tggcggacac cacctgttcg
    32041 gtgctgctga tccccgacgt ctttaccttc aacattctcc attcacgtct cgaagagatg
    32101 aacggcgttc cggtggtgcc gctgtacgat acgccgcttt ccggggttaa ccgcctgcta
    32161 aaacgtgcgg aagatattgt gctggcgacg cttattctgc tgctgatctc cccggtgctg
    32221 tgctgtattg cgctggcggt aaaactcagt tcaccaggac cggttatttt ccgccagact
    32281 cgctatggca tggatggcaa gccgatcaaa gtgtggaagt tccgttcgat gaaagtgatg
    32341 gagaacgaca aagtcgtgac tcaggcgacg cagaacgatc cgcgcgtcac caaagtgggg
    32401 aactttctgc gccgcacctc gctggacgaa ttgccgcagt ttatcaatgt gctgaccggg
    32461 gggatgtcga ttgtcgggcc acgtccacac gcggtggcgc ataacgaaca gtatcgccag
    32521 ctcattgaag gctacatgct gcgccacaaa gtgaaaccgg gcattaccgg ctgggcgcag
    32581 attaatggct ggcgcggcga aaccgacacg ctggagaaaa tggaaaaacg cgtcgagttc
    32641 gaccttgagt acatccgcga atggagcgtc tggttcgata tcaaaatcgt tttcctgacg
    32701 gtattcaagg gcttcgttaa caaagcggca tattgatatg agcttacgtg aaaagaccat
    32761 cagcggcgcg aagtggtcgg cgattgccac ggtgatcatc atcggcctcg ggctggtgca
    32821 gatgaccgtg ctggcgcgga ttatcgacaa ccaccagttc ggcctgctta ccgtgtcgct
    32881 ggtgattatc gcgctggcag atacgctttc tgacttcggt atcgctaact cgattattca
    32941 gcgaaaagaa atcagtcacc ttgaactcac cacgttgtac tggctgaacg tcgggctggg
    33001 gcttgtggtg tgcgtggcgg tgtttttgtt gagtgatctc attggtgacg tgctgaataa
    33061 cccggacctg gcaccgttga ttaaaacatt gtcgctggcg tttgtggtga tcccccacgg
    33121 gcaacagttc cgcgcgttga tgcagaagga gctcgagttc aacaaaatcg gcatgatcga
    33181 aaccagcgcg gtgctggcgg gctttacttt tacggtggtt agcgcccatt tctggccgct
    33241 ggcgatgatc gcgatcctcg gttatctggt caatagtgcg gtaagaacgc tgctgtttgg
    33301 ctactttggc cgcaaaattt atcgccccgg tctgcacttc tcgctggtgt cggtggcacc
    33361 gaacttacgc tttggtgcct ggctgacggc ggacagcatc atcaactatc tcaacaccaa
    33421 cctttcaacg ctggtgctgg cgcgtattct cggcgcgggc gtggccgggg gatacaacct
    33481 ggcgtacaac gtggccgttg tgccgccgat gaagctgaac ccgatcatca cccgtgtgtt
    33541 gtttccggca ttcgccaaaa ttcaggacga caccgaaaag ctgcgcgtca acttctacaa
    33601 gctgctgtcg gtggtgggga ttattaactt tccagcgctg ctggggctga tggtggtgtc
    33661 gaataacttc gtaccgctgg tattcggcga aaagtggaac agcattattc cggtgctgca
    33721 attgctgtgt attgtgggtc tgctgcgctc cgtgggtaac ccgattggtt cgctgctgat
    33781 ggcgaaagcg cgggtcgaca tcagctttaa attcaacgta ttcaaaacct ttctgtttat
    33841 tccggcgatt gttattggtg ggcagatggc gggcgcaatc ggcgtcacgc tcggtttcct
    33901 gctggtgcag attatcaaca ccattctgag ttacttcgtg atgattaaac cggttcttgg
    33961 ttccagttat cgccagtaca tcctgagttt gtggctgccg ttttatctct cgctgccgac
    34021 gctggtggtc agttatgtgc tgggcattgt gctgaaaggg caactggcgc tggggatgct
    34081 gctgggggtg caaatagccg cgggcgtgct ggcgtttgtg gtgatgattg tgctgtcgcg
    34141 ccatccgctg gtggtggaag tgaagcgtca gttttgtcgc agcgaaaaaa tgaaaatgct
    34201 tttacgggcg gggtgagttg ttgttttcgg ttccgacaac ggttgccggg tgcagcgtaa
    34261 aacgccttat ccatcctacg gttctgtgct cgggac
//
LOCUS       DADLFA010000021        34255 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000021 DADLFA010000000
VERSION     DADLFA010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34255
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.021"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..1948)
                     /locus_tag="JQH65_000873"
     CDS             complement(<1..1948)
                     /locus_tag="JQH65_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HAY6138364.1"
                     /translation="MSGKPAARQGDTTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQ
                     ALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGR
                     TQTFHREAAGEFSGEITGVTDGAGRHFQLVLTTQAQRAEEARQQAISGGTEPSAFPDT
                     LPGYTEYGRDNGIRLSAVWLTHDPEYPENLPAAPLVRYGWTPRGELAAVYDRSNTQVR
                     SFTYDDKYRGRMVAHRHTGRPEIRYRYDSDGRVTEQLNPAGLSYTYQYEKDRITITDS
                     LNRREVLHTQGEAGLKRVVKKEHADGSVTQSQFDAVGRLRTQTDAAGRTTEYSPDVVT
                     GLITRITTPDGRASAFYYNHHSQLTSATGPDGLEIRREYDEWGRLIQETAPDGDIIRY
                     RYDNPHSDLPCATEDATGSRKTMTWSRYGQLLSFTDCSGYVTRYDHDRFGQMTAVHRE
                     EGLSQYRAYDSRGQLIAVKDTQGHETRYEYNAAGDLTAVIAPDGSRNGTQYDAWGKAI
                     RTTQGELTRSMEYDAAGRVIRLTSE"
     gene            complement(2188..2394)
                     /locus_tag="JQH65_000874"
     CDS             complement(2188..2394)
                     /locus_tag="JQH65_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HAY6138365.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMEQAEKATGDFY"
     gene            2707..2796
                     /gene="kdpF"
                     /locus_tag="JQH65_000875"
     CDS             2707..2796
                     /gene="kdpF"
                     /locus_tag="JQH65_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HAY6138366.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            2796..4469
                     /gene="kdpA"
                     /locus_tag="JQH65_000876"
     CDS             2796..4469
                     /gene="kdpA"
                     /locus_tag="JQH65_000876"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HAY6138367.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            4492..6540
                     /gene="kdpB"
                     /locus_tag="JQH65_000877"
     CDS             4492..6540
                     /gene="kdpB"
                     /locus_tag="JQH65_000877"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HAY6138368.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADIAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSITNDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            6549..7092
                     /gene="kdpC"
                     /locus_tag="JQH65_000878"
                     /pseudo
     CDS             6549..7092
                     /gene="kdpC"
                     /locus_tag="JQH65_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
     gene            7085..9769
                     /gene="kdbD"
                     /locus_tag="JQH65_000879"
     CDS             7085..9769
                     /gene="kdbD"
                     /locus_tag="JQH65_000879"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdbD"
                     /protein_id="HAY6138369.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTDAMLEGLAVLPPKRLAYRGRHICEFDLDAALARR
                     PALILMDELAHSNALGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATIEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDTIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            9766..10443
                     /gene="kdpE"
                     /locus_tag="JQH65_000880"
     CDS             9766..10443
                     /gene="kdpE"
                     /locus_tag="JQH65_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HAY6138370.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFHLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            complement(10659..10916)
                     /locus_tag="JQH65_000881"
     CDS             complement(10659..10916)
                     /locus_tag="JQH65_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6138371.1"
                     /translation="MQKPLPVRWLIIQGLTENEHCGIQAYACNTESQEDLYAEVPLVG
                     RLTGIEEVKVEAIAMSKLHNMSCPPYMGHSAAVIFHLIYLS"
     gene            11039..13237
                     /gene="speF"
                     /locus_tag="JQH65_000882"
     CDS             11039..13237
                     /gene="speF"
                     /locus_tag="JQH65_000882"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HAY6138372.1"
                     /translation="MSKLKIAVSDSCPDCFTTQRECIYINESRNIDVAAIVLSLNDVT
                     CGKLDEIDATGYGIPVFIATENQERVPAEYLPCISGVFENCESRREFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEALFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     TKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGFDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQPQKEMFRKEHFPRV
                     SMNPQEANYAYSRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            13234..14572
                     /gene="potE"
                     /locus_tag="JQH65_000883"
                     /pseudo
     CDS             13234..14572
                     /gene="potE"
                     /locus_tag="JQH65_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
     gene            14600..14797
                     /locus_tag="JQH65_000884"
                     /pseudo
     CDS             14600..14797
                     /locus_tag="JQH65_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405579.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14921..15571
                     /locus_tag="JQH65_000885"
     CDS             14921..15571
                     /locus_tag="JQH65_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2625 domain-containing protein"
                     /protein_id="HAY6138373.1"
                     /translation="MREMNQLVNTEDSAWPIIQNWLKDATNHTELLPVNKNLAETALY
                     QLQVTTKSPMGALVYGSGGLLIDNGWLRIAGSGHPRLPRDPVSWTQRPEFAGVRALPI
                     ADDVAGGIFALNGGDLGEDTGCVYYFAPDTLNWESLEVGYSEFLQWALSGDLDTFYEN
                     VRWQQWREDVIKLSATEAFTFYPFLWVQSEEARTRKVISLTELWEMQYQMKETFTQ"
     gene            complement(15612..16106)
                     /locus_tag="JQH65_000886"
     CDS             complement(15612..16106)
                     /locus_tag="JQH65_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6138374.1"
                     /translation="MKTNRSLVVIVSLITATLLLTACAQPEQSSLAGDWLLTPKDKTR
                     RLTGSIAVNIAPSRCKTNCRGDNLPDNTRRWQLSGGNEKELTYLHNMSAQEKVGLNPG
                     WQCYTSFFMRVCQGKPGTRPIVNEDYVSESGFFGSMMHVGIIELRRCQSENCQQELKA
                     INTH"
     gene            complement(16321..17961)
                     /gene="pgm"
                     /locus_tag="JQH65_000887"
     CDS             complement(16321..17961)
                     /gene="pgm"
                     /locus_tag="JQH65_000887"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HAY6138375.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALLADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(17987..18532)
                     /gene="seqA"
                     /locus_tag="JQH65_000888"
     CDS             complement(17987..18532)
                     /gene="seqA"
                     /locus_tag="JQH65_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HAY6138376.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIAEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            18717..19481
                     /gene="ybfF"
                     /locus_tag="JQH65_000889"
     CDS             18717..19481
                     /gene="ybfF"
                     /locus_tag="JQH65_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAY6138377.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     RDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPSGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            19552..19914
                     /gene="ybfE"
                     /locus_tag="JQH65_000890"
     CDS             19552..19914
                     /gene="ybfE"
                     /locus_tag="JQH65_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HAY6138378.1"
                     /translation="MYYGALSIRAEAWLIVSPEVTKIMAKEQTDRTTLDLFAHERRPG
                     RPKTNPLSRDEQLRINKRNQLKRDKVRGLKRVELKLNAEAVEALNELAESRNMSRSEL
                     IEEMLMQQLAALRSQGIV"
     gene            20054..20584
                     /gene="fldA"
                     /locus_tag="JQH65_000891"
     CDS             20054..20584
                     /gene="fldA"
                     /locus_tag="JQH65_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HAY6138379.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            20794..20880
                     /gene="uof"
                     /locus_tag="JQH65_000892"
     CDS             20794..20880
                     /gene="uof"
                     /locus_tag="JQH65_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HAY6138380.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            20873..21319
                     /gene="fur"
                     /locus_tag="JQH65_000893"
     CDS             20873..21319
                     /gene="fur"
                     /locus_tag="JQH65_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HAY6138381.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(21404..21730)
                     /locus_tag="JQH65_000894"
     CDS             complement(21404..21730)
                     /locus_tag="JQH65_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6138382.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQHFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(21780..23186)
                     /gene="chiP"
                     /locus_tag="JQH65_000895"
     CDS             complement(21780..23186)
                     /gene="chiP"
                     /locus_tag="JQH65_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HAY6138383.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAVFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSLGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPAYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(23633..25297)
                     /gene="glnS"
                     /locus_tag="JQH65_000896"
     CDS             complement(23633..25297)
                     /gene="glnS"
                     /locus_tag="JQH65_000896"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HAY6138384.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTRQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSSEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(25500..27446)
                     /gene="nagE"
                     /locus_tag="JQH65_000897"
     CDS             complement(25500..27446)
                     /gene="nagE"
                     /locus_tag="JQH65_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HAY6138385.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPSTAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            27779..28579
                     /gene="nagB"
                     /locus_tag="JQH65_000898"
     CDS             27779..28579
                     /gene="nagB"
                     /locus_tag="JQH65_000898"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAY6138386.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            28639..29787
                     /gene="nagA"
                     /locus_tag="JQH65_000899"
     CDS             28639..29787
                     /gene="nagA"
                     /locus_tag="JQH65_000899"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAY6138387.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLARHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            29796..31016
                     /gene="nagC"
                     /locus_tag="JQH65_000900"
                     /gene_synonym="nagR"
     CDS             29796..31016
                     /gene="nagC"
                     /locus_tag="JQH65_000900"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HAY6138388.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            31064..31816
                     /gene="nagD"
                     /locus_tag="JQH65_000901"
     CDS             31064..31816
                     /gene="nagD"
                     /locus_tag="JQH65_000901"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HAY6138389.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETSSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            32213..33877
                     /gene="asnB"
                     /locus_tag="JQH65_000902"
     CDS             32213..33877
                     /gene="asnB"
                     /locus_tag="JQH65_000902"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HAY6138390.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSNQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
BASE COUNT         8047 a         8772 c         8945 g         8491 t
ORIGIN      
        1 tttcactggt caggcggatg acccgtcctg cagcatcgta ttccatactg cgcgtcagct
       61 cgccctgcgt ggtacggatg gcctttcccc acgcatcgta ctgtgtcccg tttctgctgc
      121 cgtccggggc aatgacggcg gtcaggtcac cggcggcgtt gtattcatac cgcgtttcat
      181 ggccctgcgt gtctttcacg gcaattaact gtccacggct gtcgtatgcg cggtactgac
      241 tcagcccttc ctcgcggtgc accgccgtca tctgcccgaa gcggtcatgg tcataacggg
      301 ttacataacc ggaacagtcg gtgaagctca gcaactgacc gtaacggctc cacgtcatgg
      361 ttttccggct accggtggca tcttccgttg cgcagggtaa gtcactgtgt ggattatcat
      421 aacggtagcg gatgatatcg ccgtcagggg cagtttcctg aatcagacgg ccccattcat
      481 catattcccg gcgtatttcc agcccgtcag gcccggtggc tgacgttaac tggctgtggt
      541 ggttatagta aaacgccgat gccctgccat ccggcgtggt gatgcgcgtg atgaggcccg
      601 tcaccacatc cgggctgtat tctgttgtcc ggcctgcggc atccgtctgt gtcctgagcc
      661 tgcccacggc gtcaaactga ctctgcgtga cgctgccgtc cgcgtgttcc tttttcacca
      721 cccgcttcag cccggcttca ccctgcgtgt gcagcacttc acggcggttc aggctgtcgg
      781 tgatggtgat gcggtccttc tcatactgat acgtgtagct taagcctgcc gggttaagct
      841 gttctgtcac ccgcccgtcg ctgtcgtaac ggtaacggat ttccggccgg cccgtgtgac
      901 ggtgcgccac catccggccc cggtatttat catcgtaagt aaagctacgc acctgtgtat
      961 tgctgcggtc atacactgcc gccagttcgc cgcgcggcgt ccagccatag cgcaccagcg
     1021 gcgcggcagg taaattctcc gggtattccg ggtcgtgcgt cagccacacg gcagacagac
     1081 ggatgccgtt gtcccggcca tattcggtgt aacccggcag ggtatcagga aaagcggacg
     1141 gttccgtccc gccggaaatg gcctgctgcc gggcttcttc tgcccgctgc gcctgcgtgg
     1201 tcagtaccag ttggaagtga cgcccggcac catccgtcac gccggtgatt tcgccgctga
     1261 attcaccggc ggcttcgcgg tggaacgtct gtgtgcgccc gaagcggtcc accagcccgg
     1321 tcagtacccg gtacggcggc agcggcgcag gcagcacctc atccgcttcc ggcacccgct
     1381 cacaccagcc gagcagccac cacggcccct gcggactgtt tgtcgccaga taacgatgcg
     1441 gacttaagcg gagttcttcc ggcagcgcct gccagagtgc ggccagccgg tgaccttcat
     1501 ccagtttcgc cacgccgccg cgcaccagcc acagtgactc gctgcggctg taaccgtcct
     1561 caccgggaaa caggtgctca aaatacaggc ttctgccgcc gttatcactg agtatcagtg
     1621 tgttatcgcg cagctgtaag cggatatccg caggcatttt ccagccgggg ccgaggctcc
     1681 ccaccggcgc gggcgttttt gtccggtaac tgctgtaggt gcgggagagg atgaacggca
     1741 gcgggccggg cagggcgatg tcggtttcac cgggaaggac ctttgcaccg agcagcggat
     1801 tgaccggatg gccggacgtc accccgccgg ggcacaccga acaggccacg ccggtggggg
     1861 caccaatgcg caccccggct gaaccctgaa caatgctacc gccatactgc gtcgtgtcgc
     1921 cctgacgcgc tgccggtttt ccgctcattt ctcatccttg ttttatcggg ttatttgtcc
     1981 gctgacaggc aaaaccctac agatttataa gaaagcctta agtgagatat ccatcagata
     2041 gaaacaatta ggaaaatatt tatatatttt tcctgaatat atttcagctg ggtaatacca
     2101 tatctaaata tttacctgaa catcttccta ataaaaaaac cgccagcatc tccactagcg
     2161 gttttcaatg tattcaaaac tcggcaatca gtaaaaatca ccggttgcct tttcggcctg
     2221 ttccatccaa accggtttat cgctggtttt cgaccagacg cggtgcaggt agctgtaaaa
     2281 gcgggcgcgg tctttccaga aaagcatgaa aggcagcgcc agaacgcccg ctgcaaccgc
     2341 atatgtacgg cgtaaaaaga taagccatgc aggatattct ctgtagagtt ccatactttt
     2401 ctcccccttt tgtatctacc cggtgaatag caccggaaaa atgaatttgt ttatctgatg
     2461 aaaatagtac cgccttttgt gtaattttac tactcatccg accacttatt tttgcttatt
     2521 gatggtttat ttacattcac tctgtaatta agttgcataa aagttaaatt aatgctaaac
     2581 attagttaaa ccatggcttt tgccattttt atactttttt tacaccccgc ccgcagattt
     2641 ttgcgaaatc tttgcagcca gaattctact cttccggtat cacttttagg ccactggagg
     2701 tgcactgtga gtgcaggcgt gatagccggc gtgttgctgg tgtttttatt actgggttat
     2761 ttggtttatg ccctgatcaa tgcggaggcg ttctgatggc tgcgcaaggg ttcttactga
     2821 ttgccacgtt tttactggta ttaatggtgc tggcgcgtcc tttaggcagc gggctggcgc
     2881 ggctgattaa tgacattcct cttccaggta cagcgggcgt tgaacgtata ctctttcgtt
     2941 tacctggcgt ctctgaccat gagatgaact ggaagcaata tctttgtgcc attctcggcc
     3001 tgaacatgct ggggctggcg gtgctgtttt ttatgttgct cggtcagcac tatctgccgc
     3061 ttaatccaca gcagttgccg gggctgtcgt gggatctggc gctgaatacc gccgtcagct
     3121 ttgtcacgaa taccaactgg caatcttata gcggtgaaac cacgttgagc tatttcagcc
     3181 agatggcagg cttaacggta caaaactttc tttctgccgc cagcgggatt gcggtgattt
     3241 ttgccttcat ccgtgcgttt actcgccaga gcatgagcac actcgggaat gcctgggtcg
     3301 atctgctacg catcacgtta tgggtgctag tccctgtggc gttgttgatc gcactgtttt
     3361 ttattcaaca aggtgcgctg caaaactttc tgccctatca ggcagtgaat accgttgaag
     3421 gagcgcaaca gctgttaccc atgggaccag tagcttctca ggaagcgatc aagatgctcg
     3481 gtactaacgg cggtggcttc tttaatgcca actcgtcgca tccgtttgaa aacccaaccg
     3541 cactgaccaa cttcgtgcag atgctggcga tcttcttgat cccaacagct ctgtgctttg
     3601 cctttggtga agtgacgggc gatcgccgcc aggggcgcat gttgctgtgg gcgatgtcag
     3661 tgatttttgt catctgcgta ggcgtggtta tgtgggcaga agttcagggt aatcctcatc
     3721 tgctggcgct cggcgcagac agcagcatca atatggaagg taaagagagc cgtttcggcg
     3781 tgctggtcag tagcctgttt gcggtcgtga ctacggcggc gtcctgtggc gcggtgattg
     3841 cgatgcatga ttcgtttacc gctctcggcg gcatggtgcc gatgtggcta atgcaaattg
     3901 gtgaagtggt gttcggcggt gtcggttctg gtctttacgg catgatgctg tttgtcctgc
     3961 tggcggtgtt tattgccggg ctgatgattg gtcgtacacc ggaatatctg ggtaaaaaaa
     4021 tcgacgtacg cgagatgaaa ctgactgcac tggcgattct ggttactcca acactggtac
     4081 tgatgggcgc ggcgttggcg atgatgaccg acgccggacg tagcgccatg cttaaccctg
     4141 gcccgcatgg ttttagcgaa gtgctgtacg ccgtgtcgtc cgccgctaac aacaacggca
     4201 gcgcctttgc cggattaagc gccaactctc cgttctggaa ctgtttactg gcgttctgca
     4261 tgtttgtcgg tcgcttcggg gtgattatcc cggtgatggc aattgccggt tcgctggtga
     4321 gtaaaaagag ccaacccgcc agctccggca cgctgccaac gcacggcccg ctgtttgttg
     4381 gcctgttaat cggcaccgtg ttgctggttg gcgcactgac ctttatccct gccctggcgc
     4441 ttggtccggt ggcggaatat ctctcctgat gatattgagt gagcactgaa tatgagtcgt
     4501 aaacaactgg cgctattcga accaacactt gtcgttcagg cgctgaaaga agcggtgaaa
     4561 aaattaaacc cgcaggcgca atggcgcaat ccggtgatgt ttatcgtctg gatcggcagt
     4621 ctgctgacca cctgtattag catcgcgatg gcaagcggtg cgatgcctgg caatgcgctg
     4681 tttagcgcgg caattagcgg ctggctgtgg gtcaccgtac tgttcgctaa tttcgccgag
     4741 gcgctggcag aaggccgcag taaagcgcag gccaacagtc ttaaaggggt gaaaaaaact
     4801 gcctttgccc gcaagctgcg tgagccgaaa tatggcgctg cggcggacaa agttcctgcc
     4861 gatcaactgc gtaaaggtga catcgtgctg gtagaagctg gcgatattat cccctgcgat
     4921 ggtgaagtca ttgaaggcgg cgcatcggtc gatgaaagcg ccatcaccgg ggaatcggca
     4981 ccggtgatcc gtgaatccgg cggcgatttt gcctccgtca ccggcggcac gcgtattctt
     5041 tctgactggc tggtgattga gtgtagcgtt aaccccggtg agacatttct ggatcggatg
     5101 atcgcgatgg tggaaggcgc acagcgacgc aaaacaccga acgagattgc cctgactatc
     5161 ctgctgattg ctctgaccat cgtcttttta ctggcaaccg ccacgctgtg gccgttttcc
     5221 gcgtggggcg gtaatgccgt gagtgtaacg gtactggtgg cgctgctggt ctgtctgatc
     5281 ccaaccacta ttggcggcct gttgtcagcg atcggcgtcg ccgggatgag tcggatgctg
     5341 ggcgcgaatg tgattgccac cagcggacgt gcagttgaag cggcaggtga cgttgacgtt
     5401 ctgctactgg ataaaaccgg caccatcaca ctcggtaacc gtcaggcgtc ggagtttatc
     5461 cctgcgcagg gcgtggatga aaaaacgctg gctgacgccg cacaactggc ttcgctggct
     5521 gatgaaacgc cggaaggccg cagtattgtg atcctcgcca agcaacgttt taacctgcgc
     5581 gagcgcgatg tgcagtcgct ccatgccacc tttgtaccgt ttactgcgca aagccggatg
     5641 agcgggatca acatcgacaa ccgcatgatc cgtaaaggtt ctgtcgatgc cattcgccgc
     5701 catgttgagg ctaacggcgg tcacttccct gccgatgttg atcaaaaagt cgatcaggtt
     5761 gcgcgtcagg gggccacgcc gctggtggtg gtggaaggtt cccgtgtgct gggcgttatt
     5821 gcgctgaaag atatcgtcaa aggcggtatt aaagagcgct tcgcccagct gcgcaaaatg
     5881 ggcattaaaa cggtgatgat taccggcgat aaccgtctga ctgccgccgc cattgctgcg
     5941 gaagcgggcg tcgatgattt tctcgccgaa gcgacaccgg aggccaagct ggcattgatt
     6001 cgtcagtatc aggcggaagg ccgtttggtg gcgatgaccg gcgacggcac caacgatgct
     6061 ccggcgctgg cgcaagcaga tatcgcggtg gcgatgaact ccggcaccca ggcggcgaaa
     6121 gaggcgggca atatggtcga tctcgactct aacccgacca agttgatcga ggtggtgcat
     6181 attggcaaac agatgctgat gacccgtggc tcgctgacca ccttcagcat taccaacgat
     6241 gtggcgaaat acttcgccat tattccggcg gcgttcgcgg caacgtatcc gcagttaaat
     6301 gcgctgaaca tcatgcgcct gcattcgccc gactccgcca ttctcagtgc agtcattttc
     6361 aacgccttga ttatcgtctt tttgattcct ctggcgttaa aaggcgtgag ttataaaccg
     6421 cttaccgctt ctgccatgtt gcgccgtaac ttatggattt acggtctggg tgggctgctg
     6481 gtgccgttta ttggtatcaa agtcattgat ttactgctga ccgtttgcgg tctggtgtga
     6541 ggtttaccat gcgtggctta cgtccggcat tatcgacatt tatctttctg ttattgatta
     6601 ctggcggcgt ttacccgctg ctgaccaccg tgctggggca atggtggttt ccctggcagg
     6661 ccaacggttc gttgattcgt gaaggtgata cggtgcgggg ttcggcatta atcgggcaga
     6721 attttaccgg caacggcaga aatgccctat aatccacagg cttctggcgg gagcaatctg
     6781 gcggtcagta accctgagct ggataaacaa atagccgcac gcgttgctgc attacgggcc
     6841 gctaacccgg atgccagtac gaacgttccg gttgaactgg tgacggcatc ggcaagcggg
     6901 ctggacaata atatcacccc gcaagcggcg gcctggcaga tcccacgcat cgcgaaagcg
     6961 cgtaatctca gcgttgaaca gctcacgcag ctgatcgcaa aatacagcca acaaccgctg
     7021 gtgaaatata tcggccagcc ggtcgtcaac attgtcgaac tcaatctggc gctggataag
     7081 cttgatgaat aacgaaccct tacgtcccga ccccgatcgt ctgctggaac aaactgccgc
     7141 gccgcatcgg gggaagctga aagttttctt cggtgcctgt gcaggcgtcg ggaagacctg
     7201 ggcgatgctg gcagaagccc agcgactgcg ggcgcaaggg ctggatattg tggttggcgt
     7261 ggtagaaacc cacgggcgaa aagataccga cgcaatgctg gaagggctgg ctgttctgcc
     7321 gccaaaacgc ctggcgtacc gtgggcggca catctgcgag tttgacctcg atgccgccct
     7381 cgcccgccgt ccggcgctga tcttaatgga cgaactggcg cacagtaatg cgctaggttc
     7441 tcgtcaccct aaacgctggc aggacatcga agagctgctg gaagcaggca ttgatgtttt
     7501 cactaccgtc aacgttcagc atctggaaag tctgaatgat gtggtcagcg gcgtcaccgg
     7561 aattcaggta cgggaaaccg tgcccgatcc ttttttcgat gccgccgacg acgtggttct
     7621 ggtggacttg cccccggacg atctgcgcca gcggctgaaa gaaggcaaag tctatattgc
     7681 cgggcaggcg gagcgcgcca ttgaacattt tttccgcaaa ggcaatctga tcgccctgcg
     7741 cgaactggca ctgcgccgta ctgccgatcg cgttgatgag caaatgcgcg cctggcgggg
     7801 gcatcctggc gaagagaaag tctggcacac gcgcgacgcg atccttttat gcatcggcca
     7861 taacaccggt agtgagaagc tggtccgcgc ggcggcgcgg ctggcgtcac ggctgggtag
     7921 cgtctggcac gcggtgtatg ttgaaacccc tgccctgcac cgcttaccgg aaaaaaaacg
     7981 tcgggcaatt ctcagcgcct tacgtctggc gcaggagctg ggcgcggaga cggcaacact
     8041 ttctgatcca gcggaagaaa aagcagtagt gcgttatgcc cgtgaacata atctcggcaa
     8101 gattattctc ggtcgcccgg cctcgcgccg ctggtggcgt cgggaaacgt ttgctgaccg
     8161 actggcgcgc atcgcccccg atctcgatca ggtgctggtc gcgcttgatg aaccacccgc
     8221 ccgcacgatt aacaacgcgc cggatagccg ctcttttaaa gacaagtggc gggtacagat
     8281 tcagggatgc gtggttgccg ccgcgttatg cgccgttatc actttaattg ccatgcagtg
     8341 gctgatggcg tttgatgccg ccaacctggt gatgctgtat ctgcttggcg tggtggtggt
     8401 ggcgctattt tatggacgct ggccttcggt ggttgctacc gtcattaatg tggtgagttt
     8461 cgatctcttt tttatcgccc cacgcggcac gctcgccgtc tctgatgtgc aatatctgct
     8521 gaccttcgca gtgatgttaa ccgtcgggct ggtgatcggg aaccttactg ctggcgtgcg
     8581 ttatcaggcg cgggtagccc gttaccgcga gcaacgcaca cggcacttat atgaaatgtc
     8641 gaaagctctg gcggtgggcc gcagtccgca ggacatcgcc gccaccatcg aacaatttat
     8701 tgcctccacg tttcatgccc gcagtcaggt gttgttgccc gatgacaacg gtaaattgca
     8761 gccgttaaca catccgcaag gaatgacgcc atgggacgat accatcgcgc agtggagttt
     8821 tgataaaggc ctgcctgcgg gcgcgggcac cgacacgtta cccggtgtac cgtaccagat
     8881 tttgccgcta aaaagcggcg agaaaaccta cgggctggtg gtggtggagc cggggaatct
     8941 gcgccagttg atgatcccgg aacagcagcg cctgctggag acgtttacgc tgttagtcgc
     9001 caatgccctt gagcggctga cgctaaccgc cagcgaagaa caggcgcgga tggcaagcga
     9061 acgtgaacag atccgcaacg ccctactggc agcgctttcg catgatttac gcacgccgct
     9121 cacggtgctg ttcggtcagg cagaaatctt aacgctcgat ctggcaagcg aaggatcgcc
     9181 ccacgcccgc caggccagcg agattcgtca gcatgtgctg aacactaccc gactggtgaa
     9241 taatctactg gatatggcgc gaattcagtc cggcggcttt aatttgaaga aagagtggtt
     9301 aacgctggaa gaagtggtcg gtagcgcgct gcaaatgctg gaaccgggtt tatcgtcgcc
     9361 catcaatctt tctctgccag aaccgctgac cttaatccac gttgacgggc cactctttga
     9421 acgggtgctg attaatctgc tggagaacgc ggtgaaatat gcgggtgcgc aggccgaaat
     9481 tggtatcgat gcccacgttg agggcgaaaa tctacaactg gatgtctggg ataacggccc
     9541 cggtcttccg ccaggccagg agcagacgat atttgataag tttgctcgcg ggaataaaga
     9601 gtcggcagta ccgggggtag ggcttggact ggcaatttgt cgggcgatag tggatgtaca
     9661 cgggggcact attaccgcgt tcaaccgacc ggaaggtggt gcctgttttc gtgttacact
     9721 tccccagcaa actgcccctg aacttgaaga atttcatgag gatatgtgac aaacgttctg
     9781 attgttgaag atgaacaggc tattcgtcgc tttctgcgca cggcgctgga gggcgacggg
     9841 atgcgcgtct atgaggccga aacgctgcaa cgcggcttgc tggaagcggc tacccgtaag
     9901 ccagatttga ttattctcga tctcggcctg cccgatggtg atgggattga gtttatccgc
     9961 gacctgcgcc agtggagcgc ggtgccggtg attgtgcttt ccgcacgcag cgaagagagc
    10021 gacaaaatcg ccgcgctgga tgccggagcg gatgattatc tgagtaagcc gtttggcatt
    10081 ggcgaattgc aggcccgtct gcgcgtcgct ttacgccgcc actccgctac cgccgcgccc
    10141 gatccgctgg tgaaattttc cgatgttacc gtcgatttag ccgcccgcgt gattcaccgg
    10201 ggtgaggaag aggtgcatct cacaccaatt gagttccacc tgctggcggt gctgctcaac
    10261 aatgccggaa aagtactcac ccagcgccag ctccttaacc aggtgtgggg gccaaacgcg
    10321 gtcgaacaca gtcactattt gcgtatttat atgggacatc tgcgacaaaa actggaacag
    10381 gatcccgccc gcccacgcca tttcattact gaaaccggta ttggctatcg gtttatgctt
    10441 tgaatattaa ttttaataca gcctgccttt tattaattaa agccgtaata ataaatacgg
    10501 ctttttatct taaacaacac acaaaaataa caattcaata ttttatatta ctgagtaaaa
    10561 agctcatcat taaataaatt aagactgatc attttttatt gatcaccttc acagttcaac
    10621 cgtatttcct ggatagaata tcttcacctt cattcacatc aggaaaggta aattaaatgg
    10681 aaaataacag ccgcactatg ccccatataa ggcggacaac tcatattatg aagtttgctc
    10741 atcgcaatag cttcgacttt cacttcttca atgcccgtta gtctaccgac taagggcact
    10801 tcagcgtaca ggtcttcctg actctctgta ttacaggcat atgcctgtat tccgcagtgc
    10861 tcattctctg ttaacccttg tattatcagc caccggactg gtaagggttt ctgcattctg
    10921 aaaaaagcaa tctacctgat ttatttatca gcggggtaac tttgcttttt tccggcacga
    10981 tcgatttctc attcgagaaa ttgaggacct gctattacct aaaataaaga gatgaaaaat
    11041 gtcaaaatta aaaattgcgg ttagtgattc ttgcccggac tgttttacca cgcagcgaga
    11101 atgtatctac attaatgaaa gtcgtaatat cgatgtggcg gcaatcgttt tatcgctcaa
    11161 cgatgttaca tgcggaaaac tcgatgaaat cgatgccacg ggttatggca tcccggtatt
    11221 tattgctact gaaaatcaag aacgtgtacc cgcagagtat ttgccctgta tttcgggtgt
    11281 ctttgagaat tgcgaatcgc gacgagaatt ttatggtcgt cagttagaaa ccgctgccag
    11341 ccattatgaa actcaactgc gcccaccttt cttccgcgca ctggtcgatt atgtcaatca
    11401 aggtaacagc gcgtttgatt gccctggtca tcagggcggc gaatttttcc gtcgccatcc
    11461 ggcggggaat cagtttgtgg aatactttgg tgaggcgctg ttccgttccg acttgtgcaa
    11521 cgcagacgta gcaatgggtg atctgctgat tcacgaaggc gcgccatgca ttgcacagca
    11581 acatgcggca aaagtgttca atgccgataa aacatacttc gttctaaatg gcacttcatc
    11641 ttctaacaaa gtggttttaa acgccctgct gacaccgggt gatctggtgc tgtttgaccg
    11701 caataaccac aaatctaacc accacggagc gttgctacag gctggtgcaa caccggttta
    11761 tctggaaacc gcacgtaacc cgtatggctt tatcggtggc attgatgcgc actgttttga
    11821 agaaagttac ctgcgtgagc tgatcgcgga agtcgcaccg cagcggacaa aagaggctcg
    11881 tcctttccgc ctcgccgtta ttcagctagg cacctacgac ggtacgattt ataacgcccg
    11941 ccaggtggtg gataaaattg gtcatctgtg tgactacatt ctgtttgact ccgcatgggt
    12001 cggctatgag caatttatcc cgatgatggc tgactgttca ccgctgttgc tggatcttaa
    12061 tgagaacgat ccgggcattc tggttacgca atctgtgcat aaacagcagg ctggtttttc
    12121 tcaaacttca caaatccata aaaaagacag ccacatcaaa gggcaacagc gttatgtacc
    12181 gcacaaacgc atgaacaacg cctttatgat gcatgcctcc accagcccgt tctatccgct
    12241 gtttgccgca ctggatatca acgccaaaat gcatgaaggt gtcagcggtc gtaatatgtg
    12301 gatggattgt gtggtaaatg gcattaatgc ccgcaagctg atcctcgata actgtcagca
    12361 tattcgtccg ttcgtacctg aactagtgga tggtaaaccc tggcagtcgt atgaaacagc
    12421 gcaaattgcg gttgatctgc gcttcttcca gtttgtacca ggggaacact ggcactcttt
    12481 tgaaggctat gcagagaatc aatactttgt cgatccatgc aaactgttgc tgacaacccc
    12541 aggttttgat gcacgtaacg gcgaatatga agcgttcggt gtacccgcga cgattcttgc
    12601 taacttcctg cgcgaaaatg gcgtagtgcc ggaaaaatgc gatcttaact ccatcctctt
    12661 cctgctgact ccggcagaag atatggccaa acttcagcaa cttgttgccc tgctggtacg
    12721 cttcgaaaaa ctgcttgagt ccgacgcgcc attagcagaa gtgctgcctt ccatctacaa
    12781 acagcatgaa gagcgctacg ccggttatac ccttcgtcag ttatgccagg aaatgcatga
    12841 tttgtatgcc cgtcacaacg tgaaacaacc gcaaaaagag atgttccgta aggaacactt
    12901 cccacgcgta agcatgaatc cacaagaagc caactacgcc tattcacgcg gtgaagtgga
    12961 gctggttcgt ctgccggatg ccgaaggccg tatcgctgcc gaaggtgcgc ttccttatcc
    13021 tccgggtgtg ctgtgtgttg ttccgggtga aatctggggt ggtgctgttt tgcgttactt
    13081 cagcgctctg gaagaaggga tcaacctgct accaggtttt gcaccggagc tgcagggtgt
    13141 ctatatcgaa gaacatgatg gtcgtaagca agtttggtgc tatgtcatca agcctcgtga
    13201 tgcgcaaagc accctgttga aaggggaaaa attatgagtc aggctaaatc gaacaaaatg
    13261 ggcgtcgttc agttaaccat actgacgatg gtcaacatga tgggctccgg tatcatcatg
    13321 ctgccgacaa agcttgccga agttgggaca atctcaatta tctcctggct ggtgacagcc
    13381 gtaggctcaa tggcactggc atgggcattc gcaaaatgcg gtatgttcag ccgtaaatca
    13441 ggcggtatgg gcggttatgc ggagtatgct ttcggtaagt ccggcaattt tatggcgaac
    13501 tacacctacg gtgtctcgct gctgattgct aacgtcgcga ttgctatttc ggcggttggt
    13561 tacggcaccg aactgctcgg cgcgagtttg tcgccagtgc agattggtct tgcgaccatc
    13621 ggagtgctgt ggatttgtac cgtggctaac tttggtggtg cgcgcattac cgggcaaatc
    13681 agtagcatta ctgtgtgggg agtcattatt ccggtcgtcg gtctgtgcat tattggctgg
    13741 ttctggttta gtccgacgct gtacgctaac tcatggaacc cgcaccatgt gccgttcttt
    13801 agcgctgtag gttcttccat cgccatgacg ttgtgggctt tcctcggtct ggagtcagcc
    13861 tgtgccaacg ccgaagtagt ggaaaacccg gaacgtaacg tgccgattgc agttcttggc
    13921 ggtactttag gtgcggcggt gatttatatc gtctccacca acgtgattgc cgggattgtg
    13981 cctaatatgg agctggcaaa ttcaacggca ccatttggcc tggctttcgc acagatgttc
    14041 acaccagaag tgggtaaagt cattatggcg ctgatggtga tgtcctgctg cggttcactg
    14101 cttggctggc ggttcactgc ttggctggca gttcaccatt gcccaggtgt ttaaatcttc
    14161 atctgatgaa ggctacttcc ctaaaatttt ctcccgtgta accaaagtgg atgcaccggt
    14221 gcagggaatg ttgaccattg tgattattca gagtggattg gcactgatga ccattagccc
    14281 gtcactgaac agccagttca acgtgctggt taacctggcc gtggtgacca atatcattcc
    14341 gtatattctg tcaatggcgg cattagtcat tattcagaag gtcgctaatg tgcccccctc
    14401 aaaagcgaaa gtagcaaact ttgttgcctt tgttggtgcg atgtacagtt tctatgcgct
    14461 gtactcatcc ggggaagaag ccatgctgta cggttctatc gtgaccttcc ttggttggac
    14521 actgtatggt ctggtatcac catgctttga actgaaaaat aaacacggtt aataaaacaa
    14581 agtaaaaaag ggcgattgaa tgatcgccct ttctgtttgt tggcaaatag ttcgttattt
    14641 atgccgcatc cggcagatgt agggtctaaa cgcgatcgaa cacagcaact atttgcgtaa
    14701 ttatatgggg catctgcgac aaaaactgga acaggatccg gcccggcctc gccattttat
    14761 tactgaaacc ggtattgggt atcggtttat gctttgaaaa aagtggcaat gtatggataa
    14821 agtcattttt tattcaaata taaggaactt aatatttaaa aaatgttcca tacaattcct
    14881 catcataaat gacaccgtag cacacaagga gcaagtagcg atgcgtgaaa tgaatcagtt
    14941 agtaaatact gaagattcag cctggccaat aatccagaac tggttaaaag acgccacaaa
    15001 ccataccgaa ctcttaccgg ttaataaaaa tttagccgaa accgcacttt atcaattaca
    15061 ggtgacaaca aaatcaccga tgggtgcgct tgtctatggc tccggaggtc tgctgattga
    15121 taatggttgg ttacgcatag caggatctgg tcacccccgg ttgccacgag atccagtaag
    15181 ttggacacaa cgccctgaat tcgcaggcgt gcgggcttta cccatcgctg acgatgtcgc
    15241 agggggaatt tttgctctta atggtggcga tcttggggaa gatactggct gcgtttatta
    15301 ttttgcgcca gatacgctga attgggaaag tctggaagtt ggatattctg agtttcttca
    15361 gtgggcatta tccggtgatt tagatacctt ctatgaaaat gtacgatggc agcaatggcg
    15421 agaagacgta ataaaattat cggcaacgga agccttcacg ttctaccctt tcttatgggt
    15481 acaaagtgaa gaagcaagaa caagaaaagt tatttcgctt actgaactct gggaaatgca
    15541 gtatcaaatg aaagaaacat tcacgcaata agacatgttc aggtccgggt ttcattcccg
    15601 gactcactat tttaatgtgt atttatcgct ttcaattctt gctggcaatt ttctgactgg
    15661 cagcgtcgta actcaataat cccgacatgc atcatcgagc caaaaaatcc gctttcgctg
    15721 acataatcct cattgactat cggtcgagtg ccgggcttgc cctggcaaac ccgcataaaa
    15781 aatgaggtgt agcattgcca accgggattt aaaccgacct tttcttgtgc tgacatatta
    15841 tgaagataag taagttcttt ttcgttcccg cctgacaact gccagcggcg ggtattatca
    15901 ggtaagttat caccacggca atttgtctta caccgggaag gtgcaatatt taccgcaatg
    15961 ctacccgtta atcttctggt tttatcttta ggggtcagta accagtcgcc agctaatgat
    16021 gattgttcag gttgggcgca tgcagtcagc agtaatgtgg cggtaattag tgacactatg
    16081 acgaccagac tcctgttggt cttcattggg atttccttga gctactcagt ttgttgacaa
    16141 agtgttcgtt gtttttgccg gatgcggcgt gaacgcctta tccggcctac aaaatctcgt
    16201 aaattcaata tattgcacca tcctcgtagg cctgataagc gtagcgcatc aggcaattct
    16261 gtgtttgtca tacgtaaaaa agggcgatct tccgaccgcc ctttttctta tttaatgtgt
    16321 ttacgcgttt ttcagaactt cgctaacaat ctcaaccgct tctttctcaa tctgcttgcg
    16381 atgttcttca ccgaggaagc tttcgcagta gatcttatat gcgtcttccg tgcctgacgg
    16441 acgcgcggcg aaccagccgt tgtcagtcat cactttcaga ccgccgatag aagcaccatt
    16501 gcccggtgcc gccgtcaggc gcgcggtgat cgggtcgcct gccagggtgc tggcgctcac
    16561 catttccgga gacagcttag acagcgccgc tttttgtgcg gaagtcgcag ctgcctgcaa
    16621 acggttgtag ctcggcgcac caaagcgttt tgccagttcg ttgtagtgtt cctgcgggtt
    16681 cttaccggtg acagcggtga tttccgccgc cagcagacac atgatgatgc cgtctttgtc
    16741 ggtggaccac ggcgtgccgt cgaaacgcag gaaggaagcc cctgcgctct cttcgccgcc
    16801 aaagccgaag ctgccgtcga acagaccatc aacaaaccat ttgaaaccta ccgggacttc
    16861 taccagcttg cggcccaagt cattgaccac acggtcgatc atcgcagaag aaaccagcgt
    16921 tttaccgaca gcaacatctt tgccccactg cggacgatgc tggaacaggt agttgatcgc
    16981 caccgccagg tagtggttcg gattcatcaa acctgccgga gtgacgatac cgtgacggtc
    17041 ataatccggg tcgttagcaa acgccagatc gaacttatca cgcagtgcca gcaggcccgc
    17101 catcgcacac tcggaggagc agtccatacg gatcgcgccg tctttatcaa ggtgcataaa
    17161 gcggaaggtt tgatcgacct gatcgttaac gatggtcagg ttgaggttgt aatactcgcc
    17221 aatacgcttc cagtattcga taccggaacc gcccagcgga tcaacgccca gcgtcaggcc
    17281 cgctttctga atcgccgcca tatcaacgat atcggccaga ccttccacaa acggctgcac
    17341 cagatcctgc tctttcacat gaccggatgc catcgcttcg tcgagggaga tacgcttcac
    17401 gcctttcagg ccatcggcca gcagtgcgtt ggccctgtct tccaccactt tggtgacgtt
    17461 ggtatcagcc gggccaccat ttggcggatt gtacttgata ccaccatctt ccggcgggtt
    17521 atgggacggt gtaatcacga taccgtctgc cagcggacca ccttttttat tgtgaaccag
    17581 gatggcattg gaaacggcag gcgttggagt gaagccattg ttttcctgca caatgacatc
    17641 aacgccgttc gctgccagca cttccagtac ggaaatgaac gcaggctcgg acagggcgtg
    17701 agtatcttta cccacatagc aagggccagt gatgccgttt ttcgcacgtt cttcagcaat
    17761 tgcctgagcg atcgccagaa tgtgcggctc gttaaagctg tggcgcgctg cactgccacg
    17821 gtgaccggaa gtaccgaatt tcaccgcgtg ctccgcattc cctgcttctg gtttcagtac
    17881 ataatattgc gccgtcagtt gggcgacgtt aatcaaatca ctctgttgtg caggttggcc
    17941 tgcacgattg tggattgcca ttgctttgtc ctttgtctgc aacgttttag atagttccgc
    18001 aaaccttctc aatcaattcc gccgggaatt gcatcgactg catgatgtgt tcgatcatgc
    18061 tgcatttacg gccggtgttg gtgttggtga tcacccaata cggcgtgcct ggcacatgtt
    18121 tcggcttggt ctgattacca tttttcagca gcgtttgttc atctgccgca aagtaaacgc
    18181 gtgtacgacc gtgcaacgat tccgttgctt cggcaaacgc ctgggcgtca agagaatata
    18241 gtgtagacaa cagcagcata aagcgattga ccgctcgctt ttgctctgcg tattcgtcag
    18301 aaagcagaag ttcacgcatt gcgcgaacct tgtctttaat cgttttgacc ggcttcgctt
    18361 cggcgatagc aggtgacgca acgcgaacct ctttcgtcac cggagcagca ggctgtgatg
    18421 cggcggaaaa tttcaacata cgccgtaaaa tgtcggatgc gctctcgccg atatgcttag
    18481 tgtggctggc aatatagctg tagagttcat catcaacttc aatcgttttc atcttaatcc
    18541 agtgcagtgt cttagctgaa tacaagtcgc caagagtata tgaataaatc ccggcagcgg
    18601 atagcgtcaa gcctggcttg cagaaaaact cggaataaac cttatttgtt gcagccctgt
    18661 ggcacaatgg tcaacataat accctaaccc gacaacgcga aaaaaagaac tttgccatga
    18721 aattgaatat ccgcgcgcaa actgcacaaa accagcacaa taattctccc atcgttcttg
    18781 tccatggtct gtttggtagc ctcgacaacc ttggcgtact ggctcgcgat ctggtaaacg
    18841 atcacaatat catccaggtt gatatgcgta accacggtct ttcaccgaga gatccggtaa
    18901 tgaattaccc ggcgatggcg caggatcttg ttgatactct ggatgcacag cagatcgaca
    18961 aagcaacatt tatcggtcac tccatgggcg gtaaagcggt aatggcactt acggcactgg
    19021 cccgcgatcg catcgataaa ctggtggcga tcgatatcgc accggtcgac tatcacgtac
    19081 gccgtcatga tgagattttc gcggctatca acgcggtcag tgaatcggac gcacaaactc
    19141 gccagcaagc agcagcaata atgcgccagc atcttaatga agaaggggtg attcagtttc
    19201 tgttgaaatc ttttgttgac ggggagtggc gctttaacgt gccggtattg tgggatcagt
    19261 atccacatat tgtgggttgg gagaaaatcc cggcatggga tcaccctgcc ctgtttatcc
    19321 ctagcggcaa ttctccgtat gttagcgagc agtaccgtga tgatttactg gcacaatttc
    19381 cacaggcacg ggcgcatgtg attgcaggcg cgggtcactg ggtccatgct gaaaaaccgg
    19441 atgcggtatt acgcgctatc cgtcgctatc tcaatgatta acgcaactga ttaaaaaccc
    19501 agcgtccgcg tcgctttccg cgcgtgggcg ttggcgtgac tcatcgcgct gatgtattat
    19561 ggcgcgctat ccatccgggc agaggcctgg ctgattgttt cccccgaagt caccaagatc
    19621 atggccaaag aacaaacgga ccgtacgaca ttagatctgt tcgcgcacga gcgtcgaccg
    19681 ggacgaccga aaactaatcc gctttcgcgc gatgaacagc tgcgtattaa taaacgcaac
    19741 cagctaaaac gcgacaaagt acgtggcctt aagcgtgtcg aactgaagct gaacgcggaa
    19801 gctgtcgagg cgctgaacga gctggcggag tcgcgcaata tgagccgtag cgaactgatc
    19861 gaagagatgt taatgcagca actggcggcg ctgcgtagtc agggcatcgt ttaaatttcc
    19921 actttcatgt agcacagtgt gcagtcctgc tcgtttgctg attccgaaag actgcctgtt
    19981 ctgctatgat tgcctttatc cgtgggcaat tttccacccc catttcaata agtttcaaga
    20041 ggttatttca ctcatggcta tcactggcat ctttttcggc agcgacaccg gtaataccga
    20101 aaatatcgca aaaatgattc aaaaacagct tggtaaagac gttgccgatg tccatgacat
    20161 tgcaaaaagc agcaaagaag atctggaagc ttatgacatt ctgctgctgg gcatcccaac
    20221 ctggtattat ggcgaagcgc agtgtgactg ggatgacttc ttcccgactc tcgaagagat
    20281 tgatttcaac ggcaaactgg ttgcgctgtt tggttgtggt gaccaggaag attacgccga
    20341 atatttctgc gacgcattgg gcaccatccg cgacatcatt gaaccgcgcg gtgcaacgat
    20401 cgttggtcac tggccaactg cgggctatca tttcgaagca tcaaaaggtc tggctgatga
    20461 cgaccacttt gtcggtctgg ctatcgacga agaccgtcag ccggaactga ccgctgaacg
    20521 tgtagaaaaa tgggttaaac agatttctga agagttgcat ctcgacgaaa ttctcaatgc
    20581 ctgatgtgat gcggcgtaga ctcatgtcta cgccgtatta atagataatg ccaatcaaaa
    20641 taattgctac aaatttgtaa cttttgctgt tgtacctgta caatgtcccg gtgttcaagt
    20701 ggccttgccg ttgtaaatgt aagctgtgcc acgtttttat taacaatatt tgccagggac
    20761 ttgtggtttt catttaggcg tggcaattct ataatgatac gcattatctc aagagcaaat
    20821 tctgtcactt cttctaatga agtgaaccgc ttagtaacag gacagattcc gcatgactga
    20881 taacaatacc gccctaaaga aagctggcct gaaagtaacg cttcctcgtt taaaaatcct
    20941 ggaagttctt caggagccgg acaaccatca cgtcagtgcg gaagatttat acaaacgtct
    21001 gatcgatatg ggtgaagaaa ttggtctggc tacggtatat cgcgtactga accagtttga
    21061 cgacgctggt atcgtcaccc gccacaattt tgaaggcggt aaatccgtat ttgaactgac
    21121 acagcaacat caccacgatc acctgatctg cctcgactgc ggcaaggtta tcgaatttag
    21181 tgatgattcc atcgaagcgc gtcagcgtga aattgccgca aaacatggca ttcgcctgac
    21241 taaccacagt ctctatcttt acggtcactg tgccgaaggc gattgccgcg aagatgaaca
    21301 tgcgcacgaa ggcaaataag ccagcctgaa tgagaaaaag ccaacctgcg ggttggcttt
    21361 tttatgcaag ggaaataaga aaagcgagag ttacagctct cacttatttg ttattactgc
    21421 gaatttcctg ccaaacttta tcacaatcgg ctttcaccgc ctgatcattt ccggtttgcg
    21481 ttgcacgcag acactgctga taatccagta cgcggacact ttcctggtcg gcaaaatgct
    21541 gatgttgttt ctctttcttc agcacgttta acacgctctg gcaggcttca attttttctg
    21601 gcgaaccttg tgcggtgttg atacaggcgc tgtatgcctc tttcagacga ctgtcttcct
    21661 gtggcgcggt tgattgcgca caagctacca gccccgatgc cattatggcg atgagaatca
    21721 gttttttcat agcaaattcc tcaaacgtgc ggcaggcaca aaacctgccg cgtcgggcat
    21781 cagaagatgg tgaatggtgc gattaccata aatttcacgt cacgctcatc ctggaagatg
    21841 ttgccgtaac caccgcccca gcttgggatg tcggagtggt tgtcgtattc ggtgaagtgc
    21901 agtttgaaca tcgtgccttt ggcgcgaccg tcctgaatgg tgtagaccgc atccaggctg
    21961 tatgcagact cttcaatagt ccggtttttg tcgtagtacg catccggatt gctctgccag
    22021 gtcgcaggtt tagcatccca tgcgtaaacg taggaagcgc cgatggcgaa gcctggaaga
    22081 ttccagtttt tcaggtcata catcgcaccg aagaagaccg ctttttcgcc gttagcgttg
    22141 aagtcggaac ggttatccca ccagatatcc aggcggccgt tggaggaagc gtaggctgga
    22201 gtcatacgtt gcaggaagta tccctgctga ccgtcagcct taacccaggt gccttcgagg
    22261 cgcaaatcca ctacgtcagc cgcccggtaa ccaaaggtca acgcttgcag ccaggcggtg
    22321 ccgtcataaa ggtcgttgac gctgcgatcg tcaactttat cgcgcgtacc atagaactgg
    22381 tagctggtgg ttaacgggct accggcgata tcaaatttgt agctggcttt ggcaaaatat
    22441 tgatcgatat acccttccgc ctgaccaaat gccgcttcca gtacgaagtt atttttgaag
    22501 tcgtatttcg ccccaaggga gtgcagataa tcaactttgg tggttttatc gttctgataa
    22561 aactcatcca tttccagatg ccacggcgct ttgtattcgt tggtccacat gtaggagaaa
    22621 ctcaacgcac cagcatcgcc gtaatcaaaa ttcgccccgg cttccgcccc ctgataagta
    22681 cctggcataa agctccagtg cggcgctaac agcgtttgac cagttggctg aatgtaacct
    22741 gccctcgccc aaaccggacc gtatttaaat ttggccgcag ctttatacag gcttataccg
    22801 cttttgtcgc cggaccagtc ttcgtcatag gctttattac tttttgaaaa cgcgatttcg
    22861 ttcgggtggg agctgtcgcc gttttccgcc atttcaatcg ccgtaaacac agcaatatca
    22921 aggccgaaca tatcagcagc ataaccggac tgaaaatcga ggttggcatt ccaggtggag
    22981 tgagaaaggt tggttttgta tttgtcgccg tcggtaacat ctttacggtc gcgttcacgc
    23041 tgccagtaat atataccgcc ggttaaggtt gaatcgtcga tgaatccttc ggcgcgagcc
    23101 tccggcattg ccataaagcc cgacattgct gtaacaccgg cgatagccag cgccagcgta
    23161 ctacgtttgc cactaaacgt acgcatgggt taatcctctt tgacgtataa attgctgcac
    23221 caaaggtgaa cagcgaaaaa aataaaaatt aaaaaagtag cattgagaaa agtctcgctg
    23281 actacaggaa tagactattt ttcgcgacgc gaattatcaa tctctttctg taaccagaat
    23341 gtaagaacat gagcaaccgc acatattttg atgatttttg ttacaaggct taagtttgcg
    23401 gttgaaaggc ttgcagaaga ataaaagcgt aaatttccag caagatgaaa gcggttacat
    23461 tttatttaat tatggtttgt gattttaaat ttattttatg gataaattgc gaagcggaat
    23521 taattcgcga agaaaagcag aaaaaacgcc gcagagtgcg gcgtttggga ttgtttgcta
    23581 tttcttgccg gatgcgacgt aaatgcctca cccggcctgg tagaacttaa aattactcgc
    23641 ctactttcgc ccaggtatca cgcagcccaa cggtgcggtt aaataccggt ttttccgctg
    23701 tggaatggcg gctgtcgagg cagaagtaac cttcacgctc aaactggaat gctttacccg
    23761 caaccgcatc tttcagcgac ggttcagcaa agccctgttt gatcaccagc gattccgggt
    23821 taatcaccga caggaaatca tccgcagcac ctgggttagg cacgctgaac agacggtcgt
    23881 acagacggat ttcaaccggc agcgcatgtg ccgcgctcac ccagtgaata acacctttga
    23941 ctttacgacc atctgccgga tctttgctta aggtgtcggc gtcataagta cagaagatgg
    24001 tggtgatatt gccttcggca tctttctcca cgcgttctgc cttaatgacg taagcattac
    24061 gcagacgcac ttctttaccc agcaccagac gtttgtactg cttgttagct tcttcgcgga
    24121 aatcggcgcg atcgatccaa atctcactgc taaacggcac ctggcggcta cccatttccg
    24181 gtttgttcgg atggttcggc atggtaacca tttcgccttc gccctgatag ttttcgataa
    24241 ccagtttcac cggatcgata accgccattg cgcgcggggc gttttcgttg agatcttcac
    24301 ggatgcagga ttccagcgac gccatctcaa tggtgttgtc ctgcctggtc acgccgatgc
    24361 gtttgcagaa ctcacgaata gaagcagcag tgtaaccacg acgacgcaga ccggaaatgg
    24421 tcggcatacg cgggtcatcc cagccttcaa cgtgcttgtc ggtcaccagc aggttcagct
    24481 tacgcttgga catcacagtg tattccagat tcaggcgcga gaattcgtac tggcgcgggt
    24541 gaacaggaat ggtgatgttg tccagcaccc agtcgtacag gcgacggttg tcctggaact
    24601 caagcgtaca cagagagtgc gtaatacctt ccagggcatc gctgatgcag tgggtgaagt
    24661 cgtacatcgg gtagatgcac cacttgttgc cagtctggtg gtgttcagca aatttaatgc
    24721 ggtacagcac cggatcgcgc atcacgataa acggtgaagc catgtcgatt ttcgcacgca
    24781 ggcaggcttt accttcttca aaaccaccgg cacgcatttt ttcgaacagc gccaggttct
    24841 cttcaacgct gcggtcgcgg tacgggctgt ttttacccgg ttgcgtcagg gtgccgcggt
    24901 attcgcggat ctgttccggc gtcagttcat caacgtacgc caggccttta ttgatcagtt
    24961 cgatcgcata ggcgtggagc tgatcaaaat aatcggagga gtaacggacg ttaccagacc
    25021 agtgaaaacc taaccactct acgtcgtttt tgatcgactc aacatactcg atatcttctt
    25081 ttaccgggtt agtgtcgtcg aaacgcaggt tgcactggcc tttatagtcc tgggcgatcc
    25141 cgaagttcag gcagatagat ttcgcatggc caatatgcag atagccattc ggctccggcg
    25201 ggaaacgggt gtgtactgtg gtgtgcttac cactggccag atcttcatcg atgatctgac
    25261 ggataaagtt agtcgggcgg gcttctgcct cactcatcgt ggattcctca aagcgtaaac
    25321 aacgtataac ggcgtatgat cttataagcg gaacaggctg acaactgtta gttttttaaa
    25381 attgataatc taacaaggat tatggggatg attgatgaaa aaaaaagcgg cagagaagat
    25441 ctccaccgct tgtcattgtt ggatgcgacg ctcaagcgtc gcatcaggca taaagcagat
    25501 tactttttga tttcatacag cggtgtttga cccgccacaa catggccctg agctttaatg
    25561 atcaagccac tgaaatcgtc gatattgctg caaaccaccg ggctaatcat cgagcgggcg
    25621 ttagcgttca ggtaatccag atccatttcc agaatcggtt gccctgcgct tacctgcgca
    25681 ccctcttcca ccagacgttt aaagccttta ccttccagcg ctacggtgtc gatacccata
    25741 tggacgacga tctccgcgcc tttttcggtt tccaggcaga acgcgtggtt ggtgttgaag
    25801 attttcacga ttgtccctgc ggctggtgat acgacgattt tatctgtcgg tttcaccgcc
    25861 acaccgtcac ccaccgcttt gctggcgaat gcttcgtcag gaacctgatc cagtgccacg
    25921 acatcaccgg taatcggcga taccagctcc gcgatagata ccgcgtttgg tacagcctgc
    25981 ggttttgcta caggcgcggc agttgacgga gttgcttcag ctgacgcagc ggctaccgga
    26041 ccacgggcaa cgactttctt catcgcatcg ccgatggatt ctgctttcgc gccaacaatc
    26101 acctgaatag tctgtttgtt cagtttcact acaccagaag cacccagacg tttacacatc
    26161 gtatcgttga cgcgggcaga gtcagccacg gtcaggcgca gacgggtgat acaggcgtca
    26221 attgctttca ggttgtcagt gccgccaacc gcagcaatat agttggtcgc cagttgattg
    26281 aggccttctt cagtgttgct gttggcttct tcagtaacga tctcatcttc tttatcttca
    26341 cgacccggcg ttttcaggtt gaacatgcgg ataaccaaac tgaacaccac gaagtagata
    26401 gcgaagaaga caacacccat caccagcagc atccagacgt tctggctggc ggccggcagg
    26461 ttatacatca acgcgtagtc gatagccccc gcagagaaag agaagccagc atggataccc
    26521 agcagtgttg ccacaaacag gctgataccg gtcagcagtg cgtgcaggag gtacagcagc
    26581 ggtgcaagga acatgaacag gaattccagc ggctcagtca caccggtcag gaacgcagta
    26641 acagcaacag aaagcagcat cccgccaacc atcggacgac gctctttcgg tgctgcgaag
    26701 tacatcgcca gcgccgcacc cggcagaccg aacatcataa tcgggaagaa gccggacatg
    26761 aacatccccg cggtgccgtc accggcatag aagcggttga tatcaccgtg gaaaaccgta
    26821 cccgccgcgt tggtgaattc accaatctgg aaccaggcga tggtgttcag cacctgatgc
    26881 agaccggttg ggatcagcag acggttgatg aaaccaaaga taccggaacc cagcgcgccc
    26941 gcagaaacga tccactcgcc tcctgcatgg atagcgtgct gtaccggcgg ccagacgtaa
    27001 ccaaaaatgg ccgccagcac caggcagaag aagccggtgg caatcggcac aaagcgtttg
    27061 ccgccgaaga agctcaggaa gtccggcagt ttaatatcgg accaacggtt ataggctgcg
    27121 ccaccaacca gaccggtaat gatacccgcc agtacaccca tgtttatttc tgggttgatg
    27181 gtcaccatcg ctttggttaa cacaaagtaa cctaccgcac ccgccagcgc cgccgcacct
    27241 gcgctgtctt tcgaccagct ggatgccaca ccgatggcga agattaatgc gaggttatca
    27301 aaaatcgcac cgcccgcctg ggcaataaac gcaacgttaa gtaaatctgg ctgaccgaat
    27361 cgcagcaaca gtgccgccac cggcagcacc gcgataggga gctgtaacgc cctaccgagt
    27421 cgctggaaaa aacctaaaat attcatctta ttccccctac gagaacccta tttggctcgt
    27481 ttaaagccgt atttttattt tgctgcaaat tgtactgccg atgttctgta atcagattgt
    27541 tagatcatct gctacagagt gtgtgaaaat ttaattcgta tcgcaaatta aacgcgtgtc
    27601 ttttgtgagt tttgtcacca aatatcgtta tcatcactcc cttttactgg ctaaaccaga
    27661 aaacttattt tatcattcaa aaaatcaggt cggattgacg cctgtctgcg caaatccaag
    27721 ttacgcttaa agatgcctaa tccgccaacg gcttacattt tacttattga ggtgaataat
    27781 gagactgatc cccctgacta ccgctgaaca ggtcggcaaa tgggctgctc gccatatcgt
    27841 caatcgtatc aatgcgttca aaccgactgc cgatcgtccg tttgttctgg gcctgccaac
    27901 tggcggcacg ccgatgacca cctataaagc gttagtcgaa atgcataaag caggccaggt
    27961 cagctttaag cacgttgtca ccttcaacat ggacgaatat gtcggtctgc cgaaagagca
    28021 tccggaaagc tactacagct ttatgcaccg taatttcttc gatcacgttg atattccagc
    28081 agaaaacatc aaccttctca acggcaacgc cccggatatc gacgccgaat gccgccagta
    28141 tgaagaaaaa atccgttctt acggaaaaat tcatctgttt atgggcggtg taggtaacga
    28201 cggccatatt gcatttaacg aaccggcgtc ttctctggct tctcgtactc gtatcaaaac
    28261 cctgactcat gacactcgcg tcgcgaactc tcgtttcttt gataacgatg tcaatcaggt
    28321 gccaaaatat gccctgactg tcggtgttgg tacattgctg gatgccgaag aagtgatgat
    28381 tctggtgctg ggtagccaga aagcactggc actgcaggct gcagttgaag gttgcgtgaa
    28441 ccacatgtgg accatcagct gtctgcaact gcatccaaaa gcgatcatgg tgtgcgatga
    28501 accttccacc atggagctga aagttaagac tttaagatat ttcaatgaat tagaagcaga
    28561 aaatatcaaa ggtctgtaat tgttatccct gccctggctc cttgctcagg gcaatatttt
    28621 ttaaaatcgg gggtcagaat gtatgcatta acccagggcc ggatctttac cggccacgaa
    28681 tttcttgatg accacgcggt tgttatcgct gatggcctga ttaaaagcgt ctgtccggta
    28741 gcggaactgc cgccagagat cgaacaacgt tcactgaacg gggccattct ctcccccggt
    28801 tttatcgatg tgcagttaaa cggctgcggc ggcgtacagt ttaacgacac cgctgaagcg
    28861 gtcagcgtgg aaacgctgga aatcatgcag aaagccaatg agaaatcagg ctgtactaac
    28921 tatctgccga cgcttatcac caccagcgat gagctgatga aacagggcgt gcgcgttatg
    28981 cgcgagtacc tggcaagaca tccgaatcag gcgttaggtc tgcatctgga aggtccgtgg
    29041 ctgaatctgg taaaaaaagg cacccataat ccgaattttg tgcgtaagcc tgatgccgcg
    29101 ctggtcgatt tcctgtgtga aaacgccgac gtcattacca aagtgaccct ggcaccggaa
    29161 atggttcctg cggaagtcat cagcaaactg gcaaatgccg ggattgtggt ttctgccggt
    29221 cactccaacg cgacgttgaa agaagcgaaa gccggtttcc gcgcggggat tacctttgcc
    29281 acccatctgt acaacgcgat gccgtatatt accggtcgtg aaccgggcct ggcgggcgcg
    29341 atcctcgacg aagctgacat ttattgcggt attatcgctg atggcctgca tgttgattac
    29401 gccaacattc gtaacgctaa acgccttaaa ggcgacaaac tgtgtctggt taccgacgcc
    29461 accgcgccag caggtgccaa cattgaacag ttcatttttg cgggtaaaac aatatactac
    29521 cgtaacggac tttgtgtgga tgagaacggt acgttaagcg gttcatcctt aaccatgatt
    29581 gaaggcgtgc gtaatctggt cgaacattgc ggtatcgcac tggatgaagt gctgcgtatg
    29641 gcgacgctct atccggcgcg tgcgattggc gttgagaaac gtctcggcac actcgccgca
    29701 ggtaaagtag ccaacctgac cgcattcaca cctgatttta aaatcaccaa gaccatcgtt
    29761 aacggtaacg aggtcgtaac tcaataagag aaagtatgac accaggcgga caagctcaga
    29821 taggtaatgt tgatctcgta aaacagctta acagcgcggc ggtttatcgc ctgattgacc
    29881 agtacgggcc aatctcgcgg attcagattg ccgagcaaag ccagcttgcc cccgccagcg
    29941 taaccaaaat tacgcgtcag cttatcgaac gcgggctgat caaagaagtt gatcagcagg
    30001 cctccaccgg gggccgccgc gctatctcca tcgtcaccga aacccgcaat ttccacgcaa
    30061 tcggcgtacg gcttggtcgt cacgacgcca ccatcaccct gtttgatctc agcagcaaag
    30121 tgctggcaga agaacattac ccgctgccgg aacgtaccca gcaaacgctg gaacatgccc
    30181 tgttgaatgc cattgctcag tttattgata gctaccagcg caaactgcgc gagctgatcg
    30241 cgatttcggt gatcctgcca gggcttgttg acccggacag cggcaaaatt cattacatgc
    30301 cgcatattca ggtagaaaac tgggggctgg tagaagctct ggaagaacgt tttaaagtga
    30361 cctgtttcgt tggtcacgat atccgtagtc tggcgctggc ggagcactac ttcggtgcaa
    30421 gtcaggattg cgaagactcc attctggtgc gtgtccatcg cggaaccggg gccgggatta
    30481 tctctaacgg gcgcattttt attggccgca acggtaacgt cggtgaaatt ggccatattc
    30541 aggtcgaacc gctgggtgaa cgctgccact gcggcaactt tggctgcctg gaaactatcg
    30601 ccgccaacgc tgccattgaa caacgggtgt tgaatctgtt aaagcagggc taccagagcc
    30661 gcgtgccgct ggacgactgc accatcaaaa ctatctgcaa agccgcgaac aaaggcgata
    30721 gtctggcgtc ggaagtaatt gagtatgtcg gtcgtcatct gggtaaaacc atcgccattg
    30781 ctatcaactt atttaatccg caaaaaattg ttattgccgg tgaaatcacc gaagccgata
    30841 aagtgctgct ccctgctatt gaaagctgca ttaataccca ggcgctgaag gcgtttcgca
    30901 ctaatctgcc ggtggtacgt tctgagctgg atcaccgctc ggcaatcggc gcttttgcgc
    30961 tggtaaaacg cgccatgctc aacggtattt tgctccagca tttgctggaa aattaatgtg
    31021 cttttatagt ggcgcttatt gttgtcaata ttctgggtag tccatgacca ttaaaaatgt
    31081 aatttgcgat atcgatggcg tgctgatgca cgataacgtc gccgtaccgg gtgcagcgga
    31141 atttttgcac gggattatgg ataaaggcct gccgctggtg ttgctgacca actatccttc
    31201 gcagactggg caagatctgg cgaaccgctt tgccaccgca ggtgtcgatg tacctgacag
    31261 cgtgttttat acctctgcga tggcgactgc cgatttcctg cgtcgccagg aaggcaagaa
    31321 agcgtatgtg gtgggcgaag gcgcactgat tcatgaactg tacaaagccg gtttcactat
    31381 taccgatgtg aaccctgatt tcgtgattgt tggcgaaacg agttcctaca actgggacat
    31441 gatgcataaa gcagcctatt tcgtcgctaa cggtgcacgt tttatcgcca ccaatccgga
    31501 cacccacggg cgcggttttt atcccgcgtg tggcgcgttg tgtgcaggca tcgaaaaaat
    31561 ctccgggcgt aaaccgttct atgttggtaa accgagtccg tggatcatcc gcgcggcatt
    31621 aaacaaaatg caggcgcatt cggaagaaac ggtgattgtc ggcgataacc tgcgtaccga
    31681 tattctggcg ggcttccagg caggtctgga aacgattctg gtgctttctg gtgtttcatc
    31741 gctcgacgat atcgacagta tgccgttccg cccaagctgg atttacccgt cggtcgctga
    31801 aatcgacgtt atctgaatag caaccacgtc tgcggacgtg gttggctctg atgacaaacg
    31861 ccaaactgcc tgatgcgcta cgcttatcag gcctacgcag ctcctgcaat atattgaatt
    31921 ttcatgcttt tgtaggccgg ataaggcgtt cacgccgcat ccggcatgaa caaattgcac
    31981 tttgtcagca atttggcccc cgtaaaatac aaacattcaa taaaacccgc tgaaaattat
    32041 cgattcatca ataaatatat cctcacacca gacctttttt caccaatcga caatcaccat
    32101 tacgtttttt attttttcac cgccaaatcg cttgcgcaca gtgctgcttt gcggcatttt
    32161 tttaaacaag caaacacaac aagcaacaaa taccagatta acggagaagg ttatgtgttc
    32221 aatttttggc gtattcgata tcaaaacaga cgcagttgag ctgcgtaaga aagcactcga
    32281 gctgtcacgc ctgatgcgtc atcgtggccc ggactggtcc ggtatttatg ccagcgataa
    32341 cgccattctc gctcacgaac gtctgtcaat tgttgacgtt aacgcggggg cgcaacctct
    32401 ctacaaccaa caaaaaaccc acgtactggc ggtaaacggt gaaatctaca accaccaggc
    32461 attgcgcgct gaatatggcg atcgttatca gttccagaca gggtctgact gtgaagtgat
    32521 cctcgcgctg tatcaggaaa aagggccgga atttctcgac gacttgcagg gcatgtttgc
    32581 ctttgccctg tacgacagcg aaaaagatgc ctacctgatt ggtcgcgacc atctggggat
    32641 catcccactg tatatgggct atgacgaaca cggtcagctg tatgtggcct cagaaatgaa
    32701 atcgctggtg ccagtttgcc gcacgattaa agagttcccg gcggggagct atttgtggag
    32761 ccaggacggc gaaatccgtt cttattatca tcgcgactgg ttcgactttg atgcagtgaa
    32821 agataacgtg accgacaaaa acgagctgcg tcaggctctg gaagattccg ttaaaagcca
    32881 tctgatgtct gatgtgcctt acggtgtgct gctttctggt ggtctggatt cctcaattat
    32941 ttccgctatc accaagaaat acgcagcccg tcgcgtggaa gatcaggaac gctctgaagc
    33001 ctggtggccg cagttacact cctttgctgt aggtctgccg ggttcaccgg atcttaaagc
    33061 agcccaggaa gtggcaaacc atctgggcac ggtgcatcac gaaattcact tcactgtaca
    33121 ggaaggtctg gatgccatcc gcgacgtgat ttaccacatc gaaacttatg atgtgaccac
    33181 tattcgcgct tcaacaccga tgtatttaat gtcgcgtaag atcaaggcga tgggcattaa
    33241 aatggtgctg tccggtgaag gttctgatga agtgtttggc ggttatcttt acttccataa
    33301 agcgcccaac gctaaagaac tgcatgaaga gacggtgcgt aaactgctgg ccctgcatat
    33361 gtatgactgc gcgcgcgcca acaaagcgat gtcagcctgg ggcgtggaag cacgcgttcc
    33421 gttcctcgac aaaaaattcc ttgatgtggc gatgcgcatt aacccgcagg ataaaatgtg
    33481 cggtaacggc aaaatggaaa aacacatcct gcgtgaatgt tttgagtcat acctgcccgc
    33541 aagcgtggcc tggcggcaga aagagcagtt ctccgatggc gtcggttaca gttggatcga
    33601 caccctgaaa gaagtggcgg cgcagcaggt ttctaatcag caactggaaa ctgcccgctt
    33661 ccgcttcccg tacaacacgc caacctcaaa agaagcgtat ctgtaccggg agatctttga
    33721 agaactgttc ccgcttccga gcgccgctga gtgcgtgcct ggcggtcctt ccgtcgcgtg
    33781 ttcttccgct aaagcgatcg aatgggatga agcgttcaag aaaatggacg atccatctgg
    33841 tcgtgcggtt ggtgttcacc agtcggcata taagtaagaa atgcgaaaca gccccggtga
    33901 aatccggggc tgtttcagtt attgaggtgc ttgttgattc tgatatgcag taaggaaatg
    33961 ttccgggttt gccacatcac tgtttgacaa ctcatcactg taggccagta tttcatagat
    34021 taatttccca ccactattgt tgaattccga atttcatcct gtacgacaaa agaatggctg
    34081 tagggtgggg aaaaaacaca aagtaaacaa taattgacga atatagcgcc acgctgttcg
    34141 caacctaacc aaatagtcac tttcgagcaa ttttccttga aaaagaggtt gacgctgcaa
    34201 ggctctatac gcataatgcg ccccgcaacg ccgataaggt atcgcgaaaa aaaag
//
LOCUS       DADLFA010000022        34118 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000022 DADLFA010000000
VERSION     DADLFA010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34118
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.022"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..110)
                     /locus_tag="JQH65_000903"
     CDS             complement(<1..110)
                     /locus_tag="JQH65_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HAY6138391.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACS"
     gene            complement(270..482)
                     /gene="rpmE"
                     /locus_tag="JQH65_000904"
     CDS             complement(270..482)
                     /gene="rpmE"
                     /locus_tag="JQH65_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HAY6138392.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            685..2883
                     /gene="priA"
                     /locus_tag="JQH65_000905"
     CDS             685..2883
                     /gene="priA"
                     /locus_tag="JQH65_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAY6138393.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPMPRQCPSCGSTHLVPVGLGTEQLEQTLAPMFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWILGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3039..4064
                     /gene="cytR"
                     /locus_tag="JQH65_000906"
     CDS             3039..4064
                     /gene="cytR"
                     /locus_tag="JQH65_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HAY6138394.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4156..5115
                     /gene="ftsN"
                     /locus_tag="JQH65_000907"
     CDS             4156..5115
                     /gene="ftsN"
                     /locus_tag="JQH65_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HAY6138395.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5208..5738
                     /gene="hslV"
                     /locus_tag="JQH65_000908"
     CDS             5208..5738
                     /gene="hslV"
                     /locus_tag="JQH65_000908"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HAY6138396.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5748..7079
                     /gene="hslU"
                     /locus_tag="JQH65_000909"
     CDS             5748..7079
                     /gene="hslU"
                     /locus_tag="JQH65_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HAY6138397.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7146..8072
                     /gene="menA"
                     /locus_tag="JQH65_000910"
     CDS             7146..8072
                     /gene="menA"
                     /locus_tag="JQH65_000910"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAY6138398.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDRPDRIGPLRGMQKGVINQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFILGIFLSQWAA"
     gene            8165..8650
                     /gene="rraA"
                     /locus_tag="JQH65_000911"
     CDS             8165..8650
                     /gene="rraA"
                     /locus_tag="JQH65_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HAY6138399.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8735..8980)
                     /gene="zapB"
                     /locus_tag="JQH65_000912"
     CDS             complement(8735..8980)
                     /gene="zapB"
                     /locus_tag="JQH65_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HAY6138400.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9406..10251
                     /gene="glpF"
                     /locus_tag="JQH65_000913"
     CDS             9406..10251
                     /gene="glpF"
                     /locus_tag="JQH65_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HAY6138401.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVKEKETTTPSE
                     QKASL"
     gene            10274..11782
                     /gene="glpK"
                     /locus_tag="JQH65_000914"
     CDS             10274..11782
                     /gene="glpK"
                     /locus_tag="JQH65_000914"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAY6138402.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            11917..12927
                     /gene="glpX"
                     /locus_tag="JQH65_000915"
     CDS             11917..12927
                     /gene="glpX"
                     /locus_tag="JQH65_000915"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HAY6138403.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            13024..13770
                     /gene="fpr"
                     /locus_tag="JQH65_000916"
     CDS             13024..13770
                     /gene="fpr"
                     /locus_tag="JQH65_000916"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HAY6138404.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13775..14203)
                     /gene="uspD"
                     /locus_tag="JQH65_000917"
     CDS             complement(13775..14203)
                     /gene="uspD"
                     /locus_tag="JQH65_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HAY6138405.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14230..14529)
                     /locus_tag="JQH65_000918"
     CDS             complement(14230..14529)
                     /locus_tag="JQH65_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HAY6138406.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14741..15181)
                     /locus_tag="JQH65_000919"
     CDS             complement(14741..15181)
                     /locus_tag="JQH65_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAY6138407.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     CFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15282..15881
                     /locus_tag="JQH65_000920"
     CDS             15282..15881
                     /locus_tag="JQH65_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HAY6138408.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            15989..16756
                     /gene="tpiA"
                     /locus_tag="JQH65_000921"
     CDS             15989..16756
                     /gene="tpiA"
                     /locus_tag="JQH65_000921"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAY6138409.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16811..17566)
                     /gene="cdh"
                     /locus_tag="JQH65_000922"
     CDS             complement(16811..17566)
                     /gene="cdh"
                     /locus_tag="JQH65_000922"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HAY6138410.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(17673..18662)
                     /locus_tag="JQH65_000923"
     CDS             complement(17673..18662)
                     /locus_tag="JQH65_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY6138411.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVMRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(18982..19944)
                     /gene="pfkA"
                     /locus_tag="JQH65_000924"
     CDS             complement(18982..19944)
                     /gene="pfkA"
                     /locus_tag="JQH65_000924"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAY6138412.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20125..21027)
                     /gene="fieF"
                     /locus_tag="JQH65_000925"
     CDS             complement(20125..21027)
                     /gene="fieF"
                     /locus_tag="JQH65_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HAY6138413.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMIDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21175..21687)
                     /gene="cpxP"
                     /locus_tag="JQH65_000926"
     CDS             complement(21175..21687)
                     /gene="cpxP"
                     /locus_tag="JQH65_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HAY6138414.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQRS
                     TQSHMFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQ
                     AEKMANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLK
                     LLSSSNSRSQ"
     gene            21837..22535
                     /gene="cpxR"
                     /locus_tag="JQH65_000927"
     CDS             21837..22535
                     /gene="cpxR"
                     /locus_tag="JQH65_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HAY6138415.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            22532..23905
                     /gene="cpxA"
                     /locus_tag="JQH65_000928"
     CDS             22532..23905
                     /gene="cpxA"
                     /locus_tag="JQH65_000928"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HAY6138416.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(24011..24685)
                     /gene="yiiM"
                     /locus_tag="JQH65_000929"
     CDS             complement(24011..24685)
                     /gene="yiiM"
                     /locus_tag="JQH65_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HAY6138417.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(24834..25799)
                     /gene="kdgT"
                     /locus_tag="JQH65_000930"
     CDS             complement(24834..25799)
                     /gene="kdgT"
                     /locus_tag="JQH65_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HAY6138418.1"
                     /translation="MRKSRGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMITGTVSI
                     LAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVEVGFFAG
                     LSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGTAGIASF
                     EPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTVIAQTGL
                     LGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPMLIAEMVPAFKP
                     MAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(26078..26698)
                     /gene="sodA"
                     /locus_tag="JQH65_000931"
     CDS             complement(26078..26698)
                     /gene="sodA"
                     /locus_tag="JQH65_000931"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HAY6138419.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            26982..28016
                     /gene="rhaT"
                     /locus_tag="JQH65_000932"
     CDS             26982..28016
                     /gene="rhaT"
                     /locus_tag="JQH65_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HAY6138420.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLLNTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     IIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(28013..28951)
                     /gene="rhaR"
                     /locus_tag="JQH65_000933"
     CDS             complement(28013..28951)
                     /gene="rhaR"
                     /locus_tag="JQH65_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HAY6138421.1"
                     /translation="MAFCNNANLLNVFVRHIANNQLRSLAEVATVAHQLKLLKDDFFA
                     SDQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADD
                     KHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVI
                     GQLEHESSQHVSFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAAS
                     LKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISD
                     ISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQKD"
     gene            complement(28935..29771)
                     /gene="rhaS"
                     /locus_tag="JQH65_000934"
     CDS             complement(28935..29771)
                     /gene="rhaS"
                     /locus_tag="JQH65_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HAY6138422.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            30059..31471
                     /gene="rhaB"
                     /locus_tag="JQH65_000935"
     CDS             30059..31471
                     /gene="rhaB"
                     /locus_tag="JQH65_000935"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAY6138423.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVDLPVAY
                     RDSRSNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACRETAQPIPESDAELAQLRGEDFSQLHIVGGGCQNTLLNQ
                     LCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDFRQVVSTTANLTTFTPNPDS
                     EIAHYVAQLHSTRQTKELCA"
     gene            31468..32727
                     /gene="rhaA"
                     /locus_tag="JQH65_000936"
     CDS             31468..32727
                     /gene="rhaA"
                     /locus_tag="JQH65_000936"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAY6138424.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWGEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISKKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDCDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKEILSQRG"
     gene            32994..33818
                     /gene="rhaD"
                     /locus_tag="JQH65_000937"
     CDS             32994..33818
                     /gene="rhaD"
                     /locus_tag="JQH65_000937"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAY6138425.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YKDNVHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            33828..>34118
                     /gene="rhaM"
                     /locus_tag="JQH65_000938"
     CDS             33828..>34118
                     /gene="rhaM"
                     /locus_tag="JQH65_000938"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAY6138426.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSEL"
BASE COUNT         8367 a         8899 c         8851 g         8001 t
ORIGIN      
        1 gaacaggcca cgccggtggg agcaccaatg cgcaccccgg ctgaaccctg aacaatgcta
       61 ccgccatact gcgtcatgtc gccctgacgc gccgccggtt ttccgctcat cgtaaaatcc
      121 ttttgaaata aataagaacc agaagcctag cgccagtcaa caacaggaaa tgataatgag
      181 ttaacacccc ggaatttttc agaatttata aaaggcaaaa ttcaggcaca aaaaaagcgc
      241 cgtgcggcgc ttttttcgga aatccggtct tatttgctgc ccgggatgtt gaaacgcttg
      301 ttgaagcggt caacacggcc accggtagca acatcacgct gtttgccagt gaagaacggg
      361 tggcacttgc tgcacacgtc gaggttcagg tcatgaccaa cagtggagcg gattttcatt
      421 acgttaccgc aagagcagct agcagtaatt tcttcgtatt tcgggtgaat atcttttttc
      481 atgggaaaac ctcggtttaa ggccgcgtcg ctcttccagc cctaacgcca gacaccacgc
      541 gatgttaaaa gtatagcttc aatacgatca tttcgtacga aggcgcgaaa tcatacagaa
      601 attaaccagc gtatgcaaac tgatccgcac tcttctacgg caatgtgtat actaacccac
      661 cgaatttcaa gtcaggatga tgctatgccc gttgcccacg ttgccttgcc cgttccgctt
      721 cctcgtacct ttgactatct gctgccagaa ggcatgacgg ttaaagccgg gtgtcgcgtg
      781 cgcgtgccgt ttggcaaaca gcaggagcgc atcggggttg tggtgtcagt tagcgatgtc
      841 agtgagctgc cgcttaacga gctgaaagcg gtagtggaag tgctggacgt tgagccggta
      901 tttactcact ccgtctggcg gttactgctc tgggcggcag attactatca tcatccgatt
      961 ggcgatgtgc tgtttcatgc cttgccgatt ttactgcgcc aggggcggcc tgcggcgaac
     1021 gcgccgatgt ggtactggtt tgccactgaa caaggccagg cggtggatct gaacagcctg
     1081 aaacgctccc ccaagcaaca acaggcgctg gcggcgttac ggcaaggcaa aatctggcgc
     1141 gaccaggtcg ccacgctcga atttaatgat gccgcgttgc aggcgctacg caaaaaaggt
     1201 ctgtgtgatt tagcaagtga aacaccagag tttagcgact ggcgaacgaa ctatgccgtt
     1261 tctggtgagc ggttgcgatt gaataccgaa caggccaccg ccgttggcgc aattcatagc
     1321 gcggcagata ctttttctgc ctggctgctg gcgggcgtta ccggttccgg taaaacggag
     1381 gtttatctca gtgtactgga aaacgtgctc gctcagggca aacaggcgct ggtgatggtg
     1441 ccggaaatcg gcctgacacc gcaaactatc gcccgttttc gtgaacgttt taatgccccc
     1501 gtggaagttc tgcattccgg cctgaacgac agcgagcggc tttctgcgtg gttgaaagcg
     1561 aaaaatggcg aggcggcgat tgtgatcggc acccgctccg cgctgtttac gccgtttaaa
     1621 aatctcggcg tgattgtcat tgatgaagag cacgacagct cctacaagca gcaggaaggc
     1681 tggcgctatc atgcccgcga cctagcggtg tatcgtgcgc acagcgagca aatcccgatt
     1741 attcttggct ccgcaacgcc cgcgctggaa acgttatgca acgtccagca gaaaaaatac
     1801 cgcctgctgc gcctgacccg tcgggcaggg aatgctcgtc cggcaattca acatgtgctg
     1861 gatttaaaag gtcagaaggt gcaggcaggt ctggctccgg cgttaatcac ccgtatgcgc
     1921 cagcatttac aggccaacaa tcaggttatc ctctttctta atcgtcgggg ttttgcgcct
     1981 gcgctgctgt gccacgactg cggctggata gccgaatgcc cacgttgcga tcactactac
     2041 acgctgcatc aggcgcaaca gcatttgcgc tgtcaccact gtgacagtca gcgtccgatg
     2101 ccgcgccagt gcccttcctg cggttccacg cacctggtcc ccgtggggct gggcaccgaa
     2161 cagcttgaac agacgcttgc gccgatgttc cccggcgtgc ccatttcacg tatcgaccgc
     2221 gataccacca gccgcaaagg ggcactggaa cagcaactgg cggaagtgca tcgcggcggc
     2281 gcgaggattt tgatcggcac acaaatgctg gcgaaaggtc accactttcc ggatgtgacg
     2341 ctggtggcgt tactggatgt ggacggcgcg ctgttttctg ccgattttcg ctcggcagaa
     2401 cgtttcgccc agctttacac ccaggtcgcc ggtcgtgccg ggcgtgcggg taaacagggc
     2461 gaagtggtgc tgcaaacgca ccatccggaa catcctctgt tgcaaacgtt gctctataaa
     2521 ggctacgacg cctttgccga acaggcgctg gctgagcgac gcatgatgca gctgccgccg
     2581 tggaccagcc atgtgattgt gcgtgcggaa gatcataaca atcagcacgc gccgctgttc
     2641 ctgcaacaac tgcgtaatct gatcctttcc agcccgctgg cagacgacaa actgtggatt
     2701 ctcggcccgg ttccggctct ggcacctaag cgtggtggcc gctggcgctg gcagatcttg
     2761 ttgcagcacc cttcccgcgt gcgcttgcaa cacatcatta gtggtacgct ggcgcttatt
     2821 aatacaatac cggattcccg caaggtgaaa tgggtgttgg atgtcgatcc catcgaaggc
     2881 taatgcgcaa acgttgcgag gcggatcgaa aaattcaata ttcatcacac ttttcatgaa
     2941 aattctgtaa ccgttttcac gcgctatctg ctaaaaatgt tgccgatgtg aagtaaacat
     3001 ggatgtagta cgcctgacgt accaggcgag gagtgagtgt gaaagcgaag aagcaggaaa
     3061 ctgccgcgac catgaaagac gttgccctca aggcaaaagt ctctacagcg actgtctccc
     3121 gagcattaat gaatcccgat aaagtctccc aggccacccg taatcgggtt gaaaaagcgg
     3181 cccgggaagt gggttattta ccgcagccta tggggcgcaa cgtcaagcgt aatgaatccc
     3241 gcaccattct ggtgattgtc ccggatatct gcgatccctt ctttagcgaa attattcgcg
     3301 gtatcgaagt tacggcggca aatcacggat atctggtgct gattggcgac tgtgcgcatc
     3361 aaaatcagca ggaaaaaacc tttatcgatt tgatcatcac caagcaaatt gatggcatgt
     3421 tgctgctggg ttcaaggctg ccgtttgatg ccagcattga ggaacagcgt aatctgccgc
     3481 cgatggtgat ggcgaacgaa tttgcaccgg aactggagct gcctaccgtt catatcgaca
     3541 atctgaccgc cgcatttgat gcagtaaatt atttatatga gcaagggcat aaacggattg
     3601 gctgtatagc cggtcccgaa gagatgccac tgtgtcacta ccgcctgcaa ggctatgttc
     3661 aggcgctgcg tcgctgcgga attatggttg atccacaata catcgcccgt ggcgacttca
     3721 ccttcgaagc cggaagcaaa gcaatgcaac agctgcttga tcttccacaa ccgcctactg
     3781 cagtcttctg ccatagcgat gtgatggcgc tcggcgcact ttctcaggca aaacgccagg
     3841 ggctgaaagt cccggaagac ctttccataa tcggttttga taacatcgac ctgacgcaat
     3901 tttgtgatcc gccgctgaca accatcgcgc agccgcgtta cgaaatcggt cgggaagcta
     3961 tgctgttatt gcttgatcaa atgcaggggc aacacgttgg cagtggctct cgtttaatgg
     4021 actgcgaact tatcatccgg ggatcaacac gcgcgttacc ttaaagtaaa cctttaagac
     4081 tgccgtgact ggtcaaaggc ccgccgctta agtaacatgg cgggctgacg aacgaataaa
     4141 tacagcgaaa cgatagtggc acaacgagat tatgtacgcc gcagccaacc ggcaccttcg
     4201 cggcgaaaaa agagcacctc acggaaaaag caacgaaatc tgcctgcggt ttctcccgct
     4261 atggtcgcta ttgctgccgc cgttcttgtg acctttatcg gtggtctgta cttcattacg
     4321 catcacaaga aagaagagtc cgagacgctg caaagccaga aagtgaccgg aaacggacta
     4381 ccaccaaaac cagaagaacg ctggcgctac attaaagagc tggaaagtcg ccagccgggt
     4441 gtgcgtgcgc ccacagaacc ttctgccggt ggtgaagtga aaacgccgga gcaactgaca
     4501 ccagaacaac gtcagcttct tgaacaaatg caggctgata tgcgccagca gccaacgcag
     4561 ctggttgagg ttccgtggaa tgaacagacg ccggaacagc gtcagcaaac gctacaacgc
     4621 cagcgtcagg cgcagcaatt agcggagcag caacgcctgg ctcagcagtc gcgtacgact
     4681 gaacaaagct ggcagcagca gacgcgtacg tcgcaagccg cgccggtaca ggcgcaaccg
     4741 cgccagtcta aaccagcttc tactcagcag ccgtaccagg atctgctgca aacccctgcg
     4801 cacacgaccg cgcagtcgaa gccgcagcaa gctgcaccgg ttgctcgtgc cgctgacgcg
     4861 ccaaaaccga cggcggagaa aaaagacgaa cgccgctgga tggtgcagtg cggttcgttc
     4921 agaggcgcgg aacaggcaga gacggtacgt gctcagctgg cgttcgaagg atttgactcg
     4981 aaaatcacca ccaacaatgg ctggaatcgt gtggtcattg gcccggtgaa aggcaaagag
     5041 aacgcagaca gcaccctcaa tcggttgaag atggcgggtc atacaaactg cattcggctc
     5101 gccgccgggg gttgaaaccc tcaaaatccc ccccatctat aattgcatta tgcctcgtac
     5161 ttttgtacgg ggtttgtact ctgtattcgt aaccaagggg tcagctcgtg acaactatag
     5221 taagcgtacg ccgtaacggc catgtggtca tcgctggtga tggtcaggcc acgttgggca
     5281 ataccgtaat gaaaggcaac gtgaaaaagg tccgccgtct gtacaacgac aaagtcatcg
     5341 cgggctttgc gggcggtact gcggatgctt ttacgctgtt cgaactgttt gaacgtaaac
     5401 tggaaatgca tcagggccat ctggttaaag ccgccgttga gctggcaaaa gactggcgta
     5461 ccgatcgcat gctgcgcaaa cttgaagcac tgctggcagt cgcggatgaa actgcatcgc
     5521 ttatcatcac cggtaacggt gacgtggtgc agccagaaaa cgatcttatt gctatcggct
     5581 ccggcggccc ttacgcccag gctgcggcgc gcgcgctgtt agaaaacact gaacttagcg
     5641 cccgtgaaat tgctgaaaag gcgttggata ttgcaggcga catttgcatc tataccaacc
     5701 atttccacac catcgaagaa ttaagctaca aagcgtaagg atctcccatg tctgaaatga
     5761 ccccacgcga aatcgtcagc gaactggata agcacatcat cggccaggac aacgccaagc
     5821 gttctgtggc gattgctctg cgtaaccgct ggcgtcgcat gcagctcaac gaagagctgc
     5881 gccatgaagt gaccccgaaa aatatcctga tgatcggccc gaccggtgtc ggtaaaactg
     5941 aaatcgcccg tcgtctggct aagctggcga atgcgccgtt catcaaagtt gaagcgacca
     6001 aattcaccga agtgggctat gtcggtaagg aagtggattc tattattcgc gatctgaccg
     6061 atgccgccgt gaaaatggta cgcgtccagg ctatcgagaa aaaccgttat cgcgctgaag
     6121 aactggcaga agaacgtatt ctcgacgtgc tgatcccacc tgctaaaaac aactggggac
     6181 agaccgaaca gcagcaggaa ccgtccgctg ctcgtcaggc attccgcaaa aaactgcgtg
     6241 aaggccagct tgatgacaaa gaaatcgaga tcgatcttgc cgcagcaccg atgggcgttg
     6301 aaattatggc tcctccaggc atggaagaga tgaccagcca gctgcagtcc atgttccaga
     6361 acctgggcgg ccagaagcaa aaagcgcgta agctgaaaat caaagacgcc atgaagctgc
     6421 tgattgaaga agaagcggcg aaactggtga acccggaaga gctgaagcaa gacgctatcg
     6481 acgctgttga gcagcacggg atcgtgttta tcgacgaaat cgacaaaatc tgtaagcgcg
     6541 gcgagtcttc cggtccggat gtttctcgtg aaggcgttca gcgtgacctg ctgccgctgg
     6601 tagaaggttg caccgtttcc accaaacacg ggatggtcaa aactgaccac attctgttta
     6661 tcgcttctgg cgcgttccag attgcgaaac cgtctgacct aatcccggaa ctgcaaggtc
     6721 gtctgccaat ccgcgttgaa ctgcaggcac tgaccaccag cgacttcgag cgtattctga
     6781 ccgagccgaa tgcctctatc accgtgcagt acaaagcact gatggcgact gaaggcgtaa
     6841 atatcgagtt taccgactcc ggtattaaac gcatcgcgga agcggcatgg caggtgaacg
     6901 aatctaccga aaacatcggt gctcgtcgtt tacacactgt tctggagcgt ttaatggaag
     6961 agatttccta cgacgccagc gatttaagcg gtcaaaatat cactattgac gcagattatg
     7021 tgagcaaaca tctggatgcg ttggtggcag atgaagatct gagccgtttt atcctataat
     7081 cgcgttcaat cattttcatc attgtttgat ggggctgaaa ggccccattt ttattggcgc
     7141 gtattatgac tgaacaacaa attagccgaa ctcaggcgtg gctggaaagt ttacgaccta
     7201 aaaccctccc cctcgccttt gctgcaatta tcgtcgggac ggcgctggca tggtggcaag
     7261 gtcattttga tccgctggtc gccctgctgg cactgattac cgccgggcta ttacagatcc
     7321 tttctaacct cgccaatgat tacggcgatg cggtaaaagg cagcgataga cctgaccgca
     7381 ttgggccgct acgcggcatg caaaaagggg tcattaacca gcaagagatg aaacgggcgc
     7441 tcattattac cgtcgtgctc atctgtctct ccgggctggc actggttgca gtggcgtgcc
     7501 atacgctggc tgattttgtc ggtttcctga ttcttggcgg gttgtcgatc attgccgcta
     7561 tcacctacac cgtgggcaat cgtccttatg gttatatcgg tctgggggat atttccgtac
     7621 tggttttctt tggctggtta agcgtcatgg ggagctggta tttacaggct catacattga
     7681 ttccggcact gatccttccg gcgaccgcat gcggcctgct ggcaacggca gtactgaaca
     7741 ttaataacct gcgtgatatc aatagcgacc gagaaaacgg caaaaacacg ctggtggtgc
     7801 gcttaggtga agtgaacgcg cgtcgttatc atgcctgcct gctgatgggc tctctggtat
     7861 gtctggcgct gtttaatctc ttttcgctgc atagcctgtg gggctggctg ttcctgctgg
     7921 cggcaccgtt gctggtgaag caagcccgtt atgtgatgcg ggaaatggac ccggtggcga
     7981 tgcgaccgat gctggaacgc actgtcaagg gggcgttact gactaacctg ctgtttattt
     8041 tagggatatt cctaagccag tgggcagcat aactgacaaa tatcaattaa caattgatga
     8101 ttttgccaac agcccacata gcgcgatata ctgaaaattc tcgcagcaac tgaatgttaa
     8161 gcctatgaaa tacgatactt ccgagctttg tgacatctat caagaagatg ttaacgtcgt
     8221 ggaaccgctg ttctccaact ttggcggacg ggcgtcgttt ggcggacaaa taatcacggt
     8281 aaaatgtttc gaggacaacg ggttgctgta cgatctgctc gaacagaatg gccgtggtcg
     8341 tgttcttgtc gtcgatggcg gtggttctgt tcgtcgcgca ctggtcgatg ctgaactggc
     8401 gcgtctggca gtacaaaatg aatgggaagg tctggttatt tacggcgcgg tgcgtcaggt
     8461 agatgacctg gaagagttgg atatcggcat ccaggcgatg gcggcaattc cggttggtgc
     8521 cgctggcgaa ggcattggcg aaagcgatgt ccgcgtcaat tttggcggtg taaccttctt
     8581 ctccggcgac catctttatg ccgacaatac cgggattatt ctttcagaag atccgctgga
     8641 tattgaatga taagaaaggc accgcaaggt gccttttttc tgcgttacct gttggcctac
     8701 acagtaaaga aattacgcga aagatgaagc gtaatcagac ctcttccatg cgacccagca
     8761 gggcctgcag acgttcctgc cagccgttct gctgttcttt cagatggttg ttctcacgct
     8821 ccagctcttc gcgctgatgc tgggcatttt gaacttcctg cgacagtgag ttgttttttt
     8881 ctttcagctc ttcgatttcc atctgcaaca gagtgatggt atcaatcgcc tgctgtactt
     8941 ttgcttccag tttctcaaac acttctaatg acattgtcat acctctcctg aattgcaagg
     9001 cgttgatgga taaaaaatcc tcgtcccgat taccggtgac gccttaataa atacgagcgc
     9061 actttagtta gctccgattg tatgaagccg cgccatcgct gtccagcggc acgccttgca
     9121 gattacggtt tgccacactt ttcatccttc tcctggtgac ataatccaca tcaatcgaaa
     9181 atgttaataa atttgttgcg cgaatgatct aacaaacatg catcatgtac aatcagatgg
     9241 aataaatggc gcgataacgc tcattttatg acgaagcaca cacattttaa gttcgatatt
     9301 tctcgttttt gctcgttaac gataagttaa cagcatgcct acaagcatcg tggaggtccg
     9361 tgactttcac gcatacaaca aacattaact cttcaggatc cgattatgag tcaaacatca
     9421 accttgaaag gccagtgcat tgctgaattc ctcggtaccg ggttgttgat ttttttcggt
     9481 gtgggttgcg ttgcagcact aaaagtcgct ggtgcgtctt ttggtcagtg ggaaatcagt
     9541 gtcatttggg gactgggggt ggcaatggct atctacctga ccgcaggggt ttccggcgcg
     9601 catcttaatc ccgctgttac cattgcattg tggctgtttg cctgtttcga caagcgcaaa
     9661 gttattcctt ttatcgtttc acaagttgcc ggcgctttct gtgctgcggc tttagtttac
     9721 gggctttact acaatttatt tttcgacttc gagcagactc atcacattgt tcgcggcagc
     9781 gttgaaagtg ttgatctggc aggcactttc tctacttacc ctaatcctca tatcaatttt
     9841 gtgcaggctt tcgcagttga gatggtgatt accgctattc tgatggggct gatcctggcg
     9901 ttaacggacg atggcaacgg tgtaccacgc ggccctttgg ctcccttgct gattggtcta
     9961 ctgattgcgg tcattggcgc atctatgggc ccattgacgg gttttgccat gaacccagcg
    10021 cgtgacttcg gtccgaaagt ctttgcctgg ctggcgggct ggggcaatgt cgcctttacc
    10081 ggcggcagag acattcctta cttcctggtg ccgctttttg gccctatcgt tggcgcgatt
    10141 gtaggtgcat ttgcctaccg caaactgatt ggtcgccatt tgccttgcga tatctgtgtt
    10201 gtgaaagaaa aggaaaccac aactccttca gaacaaaaag cttcgctgta atatgactac
    10261 gggacaatta aacatgactg aaaaaaaata tatcgttgcg ctcgaccagg gcaccaccag
    10321 ctcccgcgcg gtcgtaatgg atcacgatgc caatatcatt agcgtgtcgc agcgcgaatt
    10381 tgagcaaatc tacccaaaac caggttgggt agaacacgac ccaatggaaa tctgggccac
    10441 ccaaagctcc acgctggtag aagtgctggc gaaagccgat atcagttccg atcaaattgc
    10501 agctatcggt attacgaacc agcgtgaaac cactattgtc tgggaaaaag aaaccggcaa
    10561 gcctatctat aacgccattg tctggcagtg ccgtcgtacc gcagaaatct gcgagcattt
    10621 aaaacgtgac ggtttagaag attatatccg cagcaatacc ggtctggtga ttgacccgta
    10681 cttttctggc accaaagtga agtggatcct cgaccatgtg gaaggctctc gcgagcgtgc
    10741 acgtcgtggt gaattgctgt ttggtacggt tgatacgtgg cttatctgga aaatgactca
    10801 gggccgtgtc catgtgaccg attacaccaa cgcctctcgt accatgttgt tcaacatcca
    10861 taccctggac tgggacgaca aaatgctgga agtgctggat attccgcgcg agatgctgcc
    10921 agaagtgcgt cgttcttccg aagtatacgg tcagactaac attggcggca aaggcggcac
    10981 gcgtattcca atctccggga tcgccggtga ccagcaggcc gcgctgtttg gtcagttgtg
    11041 cgtgaaagaa gggatggcga agaacaccta tggcactggc tgctttatgc tgatgaacac
    11101 tggcgagaaa gcggtgaaat cagaaaacgg cctgctgacc accatcgcct gcggcccgac
    11161 tggcgaagtg aactatgcgt tggaaggtgc ggtgtttatg gcaggcgcat ccattcagtg
    11221 gctgcgcgat gagatgaagt tgattaacga cgcctacgat tccgaatatt tcgccaccaa
    11281 agtgcaaaac accaatggtg tgtatgtggt tccggcattt accgggctgg gtgcgccgta
    11341 ctgggacccg tatgcgcgcg gggcgatttt cggtctaact cgtggggtga acgctaacca
    11401 cattatacgc gcgacgctgg agtctattgc ttatcagacg cgtgacgtgc tggaagcgat
    11461 gcaggccgac tctggtatcc gtctgcacgc cctgcgcgtg gatggtggcg cagtagcaaa
    11521 caatttcctg atgcagttcc agtccgatat tctcggcacc cgcgttgagc gcccggaagt
    11581 gcgcgaagtc accgcattgg gtgcggccta tctcgcaggc ctggcggttg gcttctggca
    11641 gaacctcgac gagctgcaag agaaagcggt gattgagcgc gagttccgtc caggcatcga
    11701 aaccactgag cgtaattacc gttacgcagg ctggaaaaaa gcggtcaaac gcgcgatggc
    11761 gtgggaagaa cacgacgagt aatgtaaatg ccggatgaag cgtttatgcc gcatccggta
    11821 gtcctgaaac gtgcgggggc aaccccgcac acatcaataa tcccaccctt cccctgtgct
    11881 acacttcgcg ccattcctta ctgcttagag tttgctatga gacgagaact tgccatcgaa
    11941 ttttcccgcg tcaccgaatc tgcggcgctg gctggctaca aatggttagg acgcggcgat
    12001 aaaaacaccg cggacggcgc ggcggtaaac gccatgcgta ttatgctcaa ccaggtcaac
    12061 attgacggca ccatcgtcat tggtgaaggt gaaatcgacg aagcaccgat gctctacatt
    12121 ggtgaaaaag tcggtactgg tcgcggcgac gcggtagata ttgctgttga tccgattgaa
    12181 ggcacgcgca tgacggcgat gggccaggct aacgcgctgg cggtgctggc agtaggcgat
    12241 aaaggctgct tcctcaatgc gccggatatg tatatggaga agctgattgt cgggccggga
    12301 gccaaaggca ccattgatct gaacctgccg ctggcggata acctgcgcaa tgtagcggcg
    12361 gcgctcggca aaccgttgag cgaactgacg gtaacgattc tagctaaacc acgccacgat
    12421 gccgttatcg ctgaaatgca gcaactcggc gtacgcgtat ttgctattcc ggatggcgac
    12481 gttgcggcct caattctcac ctgtatgcca gacagcgaag ttgacgtgct gtacggtatt
    12541 ggtggcgcgc cggaaggcgt agtttctgcg gcggtgatcc gcgcattaga tggcgacatg
    12601 aacggtcgtc tgctggcgcg tcatgacgtc aaaggcgaca acgaagagaa tcgtcgcatt
    12661 ggcgagcagg agctggcacg ctgcaaagcg atgggcatcg aagccggtaa agtattgcgc
    12721 ctgggcgata tggcgcgcag cgataacgtc atcttctctg ccaccggtat taccaaaggc
    12781 gatctgctgg aaggcattag ccgcaaaggc aatatcgcga ctaccgaaac gctgctgatc
    12841 cgcggcaagt cacgcaccat tcgccgcatt cagtccatcc actatctgga tcgcaaagac
    12901 ccggaaatgc aggtgcacat cctctgattg atttgatcga ttgagccttc cagtccttcg
    12961 ggactggaat ttttttgttc ggagaacgaa gataaggcaa gtcaatcaaa acaggagaaa
    13021 aacatggctg attgggtaac aggcaaagtc actaaagtgc agaactggac cgacgccctg
    13081 tttagtctca ccgttcacgc ccccgtgctt ccgtttaccg ccgggcaatt caccaagctt
    13141 ggccttgaaa tcgacggcga acgcgtccag cgcgcctact cctatgttaa ctcgcccgat
    13201 aatcccgatc tggagtttta cctggtcacc gtccccgatg gcaaattaag cccacgattg
    13261 gcggcactga aaccaggcga tgaagtgcag gtggttagcg aagcagctgg cttctttgtt
    13321 ctggatgaag taccagattg cgaaacgcta tggatgctgg caaccggtac agcgattggc
    13381 ccttatttat cgattctgca actaggcaaa gatttagatc gcttcaaaaa tctggtcctg
    13441 gttcacgccg cacgttatgc cgccgactta agctatttgc cactgatgca agaactggaa
    13501 aaacgctacg aaggcaaact gcgcattcag acggtggtca gtcgggaaac ggcagcgggg
    13561 tcgctcaccg gacggatacc ggcattaatt gaaagtgggg aactggaaag cgcgattggc
    13621 ctgccgatga ataaagaaac cagccatgtg atgctgtgcg gcaatccaca gatggtgcgc
    13681 gatacacaac agttgctgaa agagacccgg cagatgacga aacatttacg tcgccgaccg
    13741 ggccatatga cagcggagca ttactggtaa gcggttactt atcgataaac ggcacgatga
    13801 gcaaatccgc actcatctta ttgatcatcc cgcgatatgc tggcatcaaa cggttgataa
    13861 atgagtgatg atgaccacag acaaggaggt cgcactgctc tttttgcata atttccagca
    13921 gtgtttccgg catttctccg cgttcaatac gcagttttgt cttcggccat tgaatatttt
    13981 tcgtcagttt atacagcttg ttatccgact tattcttcaa caattgaaga atatcttctg
    14041 ttgcagggaa gtagataccc ggatacaact cgcttaagcc atcatcaata tgaattaacg
    14101 tcaggtgagc gtcattatgt ctggcgagct ccagagcttt attcaccagt aaggcatctt
    14161 cttcattccc ggaaattgcc acgccaatgt gtttataagc catgtttaac tccttctaaa
    14221 gcaactccat caagctaacg aacgcagtga taactcaaaa ataatcatct cggcctggca
    14281 tgagaaagtg aaggccgcat caagctcaac ttgcccctcc agatccttaa tcttgtaggc
    14341 cacgctggcc ccttcatctg cagaggatgc agcggcggca agctctttca gtttggtcat
    14401 aaaagactct gcttcttcgc gggtggcaaa aaagcgcgaa aacttactgg tacagttgtc
    14461 gttatcaatc accgtaccga tatcaatcgc acatccttta gtactgcatt tatctacgac
    14521 atctttcata gggcacctct gtatattcgc ctctctgtcg attacgactt aagaataatg
    14581 cgccgttaag ggaagaacaa acaagtaatg atcttatgga ttaccatttt ttttgatatt
    14641 gcttaaggtg aagagttcag aaggattttc cgggtattgt catttgcatc gtggaaataa
    14701 caatcccgga aaaaaatagc agtaagagat atattaaagg ttaattactt gatttattgt
    14761 cggctttata cttcacatcc tgagtatctt taccatattt attttcgcct tgtgtgccaa
    14821 caaacgcacc aagatcgata agcatcatca ccagaatcaa cgtcgggaca aaacacccca
    14881 ccgcccattg ccagacaccc ggtaaaatcg cccagttacc cgccagcagc atccacgcca
    14941 caatcatcag aaatgcccat gcgccggaac gcccgcgatc atgcaagcgc ttaacagtta
    15001 ctgccgctgt tggccaaagc aagcacacaa ggcaaaacgc cgcggtctga atatcgagta
    15061 aatttttacc cgccagtgag aaaagcacca gcatgcctgc gaaccacagg cctatccaaa
    15121 tccagaaatc acggcgtcca atacgccctt taaatgagaa taaccattgc tgtatggtca
    15181 tgtaagttcc ttgatggttg tcttttccag gattctaccc ttttgacaag ggcgacagat
    15241 atcgttttaa tcggagccag tcataacaaa aggtactgtc aatgaagcca gggtgtacgc
    15301 tgttttttct cttatgttct gcattaaccg ttacaacaac ggcgcatgca caaacaccag
    15361 atacggcaac gaccgcgcct tatctgctgg ctggagcccc tactttcgat ctctccatca
    15421 gccagtttcg agaagacttt aacagccaga atcctagcct gccactgaac gaatttcgtg
    15481 ccatcgacag cagtcccgac aaagccaatc tcactcgtgc tgcaagtaaa attaacgaga
    15541 acttgtatgc ttctacagcg ctggagcgcg gtaccttaaa aatcaaaagc attcaaatga
    15601 cctggctacc catccagggg ccagagcaaa aagcagcgaa agcgaaagct caggaataca
    15661 tggcagcagt gatccgcaca ctcaccccat taatgaccaa aacacaaagc cagaaaaaac
    15721 tgcagtcgct gctaacggcg gggaaaaaca aacgttatta caccgagaca gaaggtgcac
    15781 tgcgttatgt tgtcgcggac aacggcgaaa aggggctgac cttcgctgtt gaaccgatta
    15841 agctggcgct atctgaatcg cttgaaggtt tgaataaatg acaaaaagca aagcctttgt
    15901 gccgatgaat ctctatactg tttcacagac ctgctgccct gcggggcggc catcttcctt
    15961 tattcgctta taagcgtgga gaattaaaat gcgacatcct ttagtgatgg gtaactggaa
    16021 actgaacggc agccgccaca tggttcacga gctggtttct aacctgcgta aagagctggc
    16081 aggtgttgct ggctgtgcgg ttgcaatcgc accaccggaa atgtacatcg atatggcgaa
    16141 gcgcgaagct gaaggcagcc acatcatgct gggtgcgcaa aacgtggacc tgaacctgtc
    16201 cggcgcattc accggtgaaa cctctgctgc tatgctgaaa gacatcggcg cacagtacat
    16261 catcatcggc cactctgaac gtcgtactta ccacaaagag tctgacgaac tgatcgcgaa
    16321 aaaattcgcg gtgctgaaag agcagggtct gactccggtt ctgtgcatcg gtgaaaccga
    16381 agctgaaaac gaagcgggca aaactgaaga agtttgcgca cgtcagatcg acgcggtact
    16441 gaaaactcag ggtgctgcgg cattcgaagg tgcggttatc gcttacgaac ctgtatgggc
    16501 aatcggtact ggcaaatctg caactccggc tcaggcacag gctgttcaca aattcatccg
    16561 tgaccacatc gctaaagttg acgctaacat cgctgaacaa gtgatcattc agtacggcgg
    16621 ctctgtaaac gcgtctaacg ctgcagaact gtttgctcag ccagacatcg acggcgcgct
    16681 ggttggcggt gcgtctctga aagctgacgc tttcgcagta atcgttaaag ctgcagaagc
    16741 ggctaaacag gcttaagtct gacaggtgcc ggatttcata tccggcactt actttcctta
    16801 actcttcgcc ttaacgcaaa atctcacact gatgatcctg aatttcctcg gctgaagcac
    16861 ggttaagcgt cagtagattt cgttgtgtcg ccagcaatac aaatgagtta tcactctgcc
    16921 gtaccatcgc cagcccgtag cttcccatat gttcccgcgc ctcaggtact tcttctgcca
    16981 gcatcataaa tgggctgcgt tgtaccagtt cgctttccgt tacccgacgc gccaggtatt
    17041 catgcccgcg caaaccacct ggcagtggca accagcggct gctgatgttc gccagattgt
    17101 tatccagttg ttcgcgcaca tcaggacgaa tacaagagat atgaatatga aaatggtttt
    17161 gcgtacgccc ggtgcgggag ttgatcgcca aagaaaccgc gcgatcggga accggctggc
    17221 cgtacttttt gctcataaaa tcacgcgcct gccaggccaa ccaaaagaag ttcggcgttg
    17281 aaggatcggt caacaaagga ctttcagtac cgttaatacg atacgttggc atcaacagat
    17341 attgcagtgg gccattaaga tcttttaaaa ccacgtatcc ggcattgggt ttgacttccg
    17401 cacatggcga aggattttga ttttgctgct gattgggcaa acattcctca aggacaatct
    17461 tacgtaatgt atccgactct tcaccggtta atttccagta accaataccg gcagccacaa
    17521 cggcgataac tatcatcacc aaaaaaagaa gacccgcttt tttcatcttt ttttccctgt
    17581 acctcaaaga gatgcaaggg taacgcaaaa tcgtgacaaa taaaaaaccc ggtcgcgaga
    17641 aaccaccggg tttgataatt atcctgggga aatcagcgtt tgctgatctg atcgaacgta
    17701 ccgccgttag caaaatgctc tttttgcgct ttcgtccagc cgccgaactc ttcatcaatg
    17761 gtgaataact tcagctttgg aaacgcattt tcgtactttt tcgctacttc agcgtcgcgc
    17821 ggacggtagt agtttttcgc ggcaatttcc tgaccttctg gcgagtagag atatttcagg
    17881 taggcttccg ccacctcttt ggtgcctttt ttctcgacca ctttatcgac caccgacacg
    17941 gttggctctg cgaggataga ctcactcggc gtgacgattt cgaatttatc tttccccagt
    18001 tcattcgctg ccagcagagc ttcgttttcc caggcgatca gtacatcgcc aatcccgcgc
    18061 tcgacaaagg tgttagtgga gccgcgcgcg ccagaatcca gaacttcgac gtttttatac
    18121 agtgcccgaa caaaatcctg tgcttttgcc tgatcgttgt tgttgtgatg cagcgcgtag
    18181 ccccaggcag ccaggtagtt ccagcgtgcg ccaccagagc ttttcggatt aggcgtgatc
    18241 accgaaacac ccggtttaat cagatcgttc cagtcatgga tctgcttcgg attgccctta
    18301 cgcaccagga aaacaatggt ggaagtgtac ggtgcggagt tatccggcag acgtttgatc
    18361 cactctttat caatccgccc gcgttccgca attgcgtcca cgtcataggc cagagccagc
    18421 gtgacaacat cagcttcaat accgttgatt accgacgtcg cttgtttacc tgaaccaccg
    18481 tgtgactgac gcatcaccac gttatcacca gtttgctgtt tccagtgggc gctgaatgcc
    18541 ttgttgtact gttcgtacaa ttcgcgcgtt ggatcatatg aaacgttaag aagctgaata
    18601 tcctttgcca taacgctggt tgccgccagc aaaaatgtta accctacgcc ccacttgttc
    18661 atcgcccgac tctcttatgt tgtgttgtga tgagcaaagc gtgccagaag gttaaccaaa
    18721 cattaaagaa taaaaaaaga ttggctataa cttgcgggta tatgttgagg gattaaaaag
    18781 gcggaagaaa tccgcctcat attgctgaca aagtgcgctt tgtccatgcc ggatgcggcg
    18841 cgaacgcctt atccggccta caaaagtttg caaattcaat aaattgcaga attcatgtag
    18901 gcctgataag cgaagcgcat caggcatttt tgcttctgtc atcggtttca ggctaaagga
    18961 atctgccttt ttccgaaatc attaatacag ttttttcgcg cagtccagcc agtcaccttt
    19021 gaacggacgc ttcatgtttt cgatagcgtc gatgatgtcg tggtgaacca gctgttcgtt
    19081 ctggatacct acacaacgac cgccgtaacc tgccagcagc agatcgatag cgtaagcgcc
    19141 catacgggaa gccagaatac ggtcgtaagg caccggagaa ccaccgcgct ggatgtggcc
    19201 cagcacagtt gcgcgggttt cacgacctgt ttctttctcg atgaaatgcg ccagttcgtc
    19261 aacatcacac atatgttcgg taatcgccac gatcgcgtgt tttttacctt tcgcgatacc
    19321 cgctttgatt tcgtttacca ggtcttcacg gctgaattca acttccggaa ccacaacgaa
    19381 ttcacagcca ccggcaatgg ccgcagccaa cgtcagatcg ccacaataac ggcccatcac
    19441 ttccaccacg gaaatacgct ggtgagaaga agaggtgtca cgcagacggt cgatcgcttc
    19501 tacaacggtg ctcagcgcag tgaagaaacc gatagtgtag tcagtgcctt tgatgtcgtt
    19561 gtcgatagtg cccggcagac cgatgcacgg gaagcccatt tcggtcagac gcattgcacc
    19621 catgtaggaa ccgtcaccgc cgataaccac cagcgcgtcg ataccacgtt ttttcaggtt
    19681 ttcgatagcc acggcgcgga tgttctcgtc gcggaattcc gggaaacgcg cagaaccgag
    19741 gaacgtaccg ccacggttga tcatgtcaga cacgctgtaa cggtctagct gtaccatacg
    19801 gtcttcatac agacccagat agccgtcata aatacccatt acttccagac cttctgtcag
    19861 cgcagaacga acaaccccgc gaattgcggc gttcatgcct ggcgcatcac cgccgcttgt
    19921 caacacaccg attttcttaa tcatgactac ctctgaactt tggaatgcaa aatgaaatct
    19981 gttgccggaa gtcttcttgc acatcgaagt gatccaacga atgtgcaaat agtataacaa
    20041 tcacttcctg ctgaattgat tcaggtcagg ccaaatggcg gtattttata cacaaaatgc
    20101 gggtctggct ctcttttata ctgattatga aagcatagac cgtttaccct ccctgggtac
    20161 gacggaacag gggtcctgat ggataattac atccgatccc ggaaaacgcc gtaaaatagc
    20221 ctgctctacc tgatccgcca ccatatgtgc ctgaaccaaa ggcagagagt cttccatttc
    20281 caaatgaatc tgaataaagc gggtcggccc tgactgccgc gtgcgaagat cgtgagcgcc
    20341 gctaacaccc ggccaggaag tcacgatatc aataatttct tgccgttcct catcaggcaa
    20401 tgcgcgatcc agtaatgact gtaccgcctc atatcccatg cgtaacgcgc tatataaaat
    20461 atagatgccg attcccaatg caaacagagc atcggcgcga tgccagccgt accaggacaa
    20521 ccccagcgcc agcagaattg cgccgttcat cataacatca gactggtaat gtagcatatc
    20581 agcccgcacc gcctggcttt gcgtccggcg caccacccaa cgctgaaacg agacaaggat
    20641 aatcgtacaa attagcgcca caattgtcac gataaccccg acgcctggat ctatcatcgg
    20701 tgttggagat atcagatgtt gaatacccgt caaaaacagg aatagtgccg aaccggagat
    20761 aaacatactt tgcgccagcg ccgcgaggga ctctgcttta ccgtgaccaa acgagtgatt
    20821 atcgtcggca ggttgcaggg aatatcgcac caccagtaaa ttcgtcaacg acgcgccgat
    20881 atccaccagc gaatccacca gcgcggcgag aatactcacc gacccggtat accaccatgc
    20941 aaaaatttta atcagcaata gcaacgaagc catcgccgtc gcagcaatcg ccgcccgact
    21001 gaccagccgt ccataagatt gattcataaa tactcccgct atcaactgac gctagtataa
    21061 cggaagcaaa tcatctgcaa tgcattaagc agcaggcaaa ttgaggataa aaaaaacccc
    21121 acatcatgtg ggggaagaca gggatggtgt ctatggcaag gaaaacaggg tttactactg
    21181 ggaacgtgag ttgctactac tcaatagctt caacgatgaa cttttttgcc attgcgtcac
    21241 gtcacgcaac tgctccattc gttgttgatg tttctcgttt aaaaccgctt gctgctccgg
    21301 cgttaacagg cgatacattt ggttgcggac ttttgccatc tcaacctgac gagcaatttg
    21361 ctcattcgcc attttttctg cctgtgcgcg cacagcgttt tcatcaaaat tttctgcggt
    21421 gacaaggcga tgcattgtct ccagttcgct aacattaaca ggaggctgtt cgtgccgggc
    21481 ctgttgcata agatctcgca tctgctgacg ctgatgttcg gttaaactta tgccgtcgaa
    21541 catatggctc tgcgtactgc gctgcgtact gcgctgcgta agttcttcac ccggatgcca
    21601 gttatcgcct gaaccgactt cagcagcgtg gcttaatgaa ctgactgcca gcgttgaagc
    21661 catgacggca gcggtaacta tgcgcatcat ttgctcccaa aatctttctg tcgcgattca
    21721 acgatagaga gtttacgatt caggctgcaa acatgcgtca gggggtgtaa aacaacgtaa
    21781 agtcatggat tagcgacgtc tgatgacgta atttctgcct cggaggtatt taaacaatga
    21841 ataaaatcct gttagttgat gatgaccgag agctgacttc cctattaaag gagctgctcg
    21901 agatggaagg cttcaacgtg attgttgccc acgatgggga acaggcgctt gatcttctgg
    21961 acgacagcat tgatttactt ttgcttgacg taatgatgcc gaagaaaaat ggtatcgaca
    22021 cattaaaagc acttcgccag acacaccaga cgcctgtcat tatgttgacg gcgcgcggca
    22081 gtgaacttga tcgcgttctc ggccttgagc tgggcgcaga tgactatctc ccgaaaccgt
    22141 ttaatgatcg tgagctggtg gcacgtattc gcgcgatcct gcgccgttcg cactggagcg
    22201 agcaacagca aaacaacgac aacggttcac cgacactgga agttgatgcc ttagtgctga
    22261 atccaggccg tcaggaagcc agcttcgacg ggcaaacgct ggagttaacc ggtactgagt
    22321 ttaccctgct ctatttgctg gcacagcatc tgggtcaggt ggtttcccgt gaacatttaa
    22381 gccaggaagt gttgggcaaa cgcctgacgc ctttcgaccg cgctattgat atgcacattt
    22441 ccaacctgcg tcgtaaactg ccggatcgta aagatggtca cccgtggttt aaaaccttgc
    22501 gtggtcgcgg ctatctgatg gtttctgctt catgataggc agcttaaccg cgcgcatctt
    22561 cgccatcttc tggctgacgc tggcgctggt gttgatgttg gttttgatgt tacccaagct
    22621 cgattcacgc cagatgaccg agcttctgga tagcgaacag cgtcaggggc tgatgattga
    22681 gcagcatgtc gaagcggagc tggcgaacga tccgcccaac gatttaatgt ggtggcggcg
    22741 tctgttccgg gcgattgata agtgggcacc gccaggacag cgtttgttat tggtgaccac
    22801 cgaaggccgc gtgatcggcg ctgaacgcag cgaaatgcag atcattcgta actttattgg
    22861 tcaggccgat aacgccgatc atccgcagaa gaaaaagtat ggccgcgtgg aactggtcgg
    22921 tccgttctcc gtgcgtgatg gcgaagataa ttaccaactt tatctgattc gtccggccag
    22981 cagttctcaa tccgatttca ttaacttact gtttgaccgc ccgctattac tgctgattgt
    23041 caccatgttg gtcagtacgc cgctgctgtt gtggttggcc tggagtctgg caaaaccggc
    23101 gcgtaagctg aaaaacgctg ccgatgaagt tgcccaggga aacttacgcc agcacccgga
    23161 actggaagcg gggccacagg aattccttgc cgcaggtgcc agttttaacc agatggtcac
    23221 cgcgctggag cgcatgatga cctctcagca gcgtctgctt tctgatatct ctcacgagct
    23281 gcgcaccccg ctgacgcgtc tgcaactggg tacggcatta ctgcgccgtc gtagcggtga
    23341 aagcaaggaa ctggagcgta ttgaaaccga agcgcaacgt ctggacagca tgatcaacga
    23401 tctgttggtg atgtcacgta atcagcaaaa aaacgcgctg gttagcgaaa ccatcaaagc
    23461 caaccagttg tggagtgaag tgctggataa cgcggcgttc gaagccgagc aaatgggcaa
    23521 gtcgttgaca gttaacttcc cgcctgggcc gtggccgctg tacggcaatc cgaacgccct
    23581 ggaaagtgcg ctggaaaaca ttgttcgtaa tgctctgcgt tattcccata cgaagattga
    23641 agtgggcttt gcggtagata aagacggtat caccattacg gtggacgacg atggtcctgg
    23701 cgttagcccg gaagatcgcg aacagatttt ccgtccgttc tatcggaccg atgaagcacg
    23761 cgatcgtgaa tctggcggta caggattggg gctggcgatt gttgaaaccg ccattcagca
    23821 gcatcgtggc tgggtgaagg cagaagacag cccgctgggc ggtttacggc tggtgatttg
    23881 gttgccgctg tataagcgga gttaaactcc gcatttgtag gcaggataag gcgtttacgc
    23941 cgcatccggc atttgagcag gatgcctgat gcgacgctga tagcgtctta tcaggcctac
    24001 actccggggg ttattttccc cacaaccggc gggaaaaatc ttcgatcttg ccgctcagtc
    24061 ggcgcttttg catcgtccgc gtccagcttt tcgataaccc ggcggcggag agtaaacggt
    24121 gatactgatc gtcatcaaac ggcatatgcc atgcgatggc ggcagcctcc tgcacggtca
    24181 catcgctgac acgggaaacc aactccagcg gcgcgtccgc agacactttc cctggcgcta
    24241 tcacgctata caaccacccc accttacctg tgttttgcat caactgcgct atatcgctga
    24301 tatcaaaatg gtaattgagt ttatagcaag gcgagcgcgg ctggctgacc tgaattaatg
    24361 cctctcccca gcggaaaata tcgcccatat aaacattact ttccgtcagg ccgtcggttg
    24421 agaggttttc accaaacgca ggcgcaacaa acaactccgc ctgttccgga aattcccgcg
    24481 cccagtagag ataatgctca cgaggataat gacacagcgc tctgtctggc ccaccatgaa
    24541 cctttttctc cgcctgctcg tcaccttcca gccccagctc cgtcagcatc aactcaccat
    24601 caacctggat tttggcaatt gcgctgggtt tgccttcggg ataagcctga atcttgcctg
    24661 tgtatacatc aaccggatat cgcatctgtt ccctctccac gagaaatttc cccattagac
    24721 catatttatt ttatgtcagg cgtgctgttt tcgcggtaag tatccaggca ggaactcaat
    24781 agcaaaccgg acgaacgtta gttcatccgg ctgtggggat taaggaagca ggtttatttc
    24841 accgtaccta aaatttcgat tttcgctgct ctggctttga ctttacgtga ccagatagag
    24901 gtaagaattg gcaccagaat cgaagtcaca atgaccgccg tcgctaccag cgaagttgct
    24961 gccggagcca tcggtttaaa cgcaggcacc atttctgcaa tcagcatagg tgtcgctacc
    25021 gcggcccctg cggaactgga agcggcaatt ccggcagtgc catcgccacc gccaatcaat
    25081 ttatcggcga taatcaacgg aataccggtc acgataatta ctgccacacc caacaggatc
    25141 cccagcaaac cagtctgggc aatcacagtc aaatcaatgg tattgcccag cgcgaaggca
    25201 aagaatggaa tcagcgtttg caccgctttg ctgaaaaatt ctcgtaattc agggtcaagg
    25261 ttccccaggg caaagcccac caggaacggt aatactgcgc cgacgaaaac atgcggttca
    25321 aacgaggcaa tccctgcagt gcccagaata atcatcgtca tgagcggccc ggactccaac
    25381 gacatcaaca caaatgcccc agcttcttct tttgtgccgt actgctgcat gatggaagcg
    25441 taaagtccgc cattggtcat atccatcgcc gccaccagcg ccagcgttga aagtccggca
    25501 aagaatccaa cttcaacacc atgttccgga ataatgcgcg aggcaattgc cgcaaccacc
    25561 cacgcgacgg caatttttgt taccaccagc gtaccggatt tacgcaatac cgttcccgtc
    25621 gcgcttaatt ttattgacgc ccccatgcaa aaaaaccaca ccgccagaat ggacaccgta
    25681 ccggtaatca tcccgttggt gaatgatcca aaatatttcc ccgcccccgg cgagaaggtg
    25741 tggcacagtg cgccaaggaa tagcgggacg agcatcatcc ccccccggga ttttctcaat
    25801 cgagcgtttt atctgcattt ccatcacctg tcggcatatt tttaagggct gatgcgtaat
    25861 tcctcaatgg agcatcagtg ttgatggaaa cgaagataga cattcattta atcaataaaa
    25921 gtgatcaaaa ccacatcaat tgaaacgctg ttttattttt atcggatcat tgtttttatt
    25981 ttgaaattga tcacaaaaaa accaccgccg ttggcgatgg ttcatttttt aagctgatat
    26041 gcggcgtaaa cgcctcattg cagcaggcgg caaatgatta ttttttcgcc gcgaaacgtg
    26101 ccgctgcttc atcccagttc accacgttcc agaactcttt gatgtagtcc ggacggcggt
    26161 tctggaattt caggtagtaa gcatgttccc acacatccag gcccagaatc gggaagccgg
    26221 aagcgccaga aatagcttca cccatcagcg gggaatcctg gttagcagta gaaaccaccg
    26281 ccagtttatc gcctttcagc accagccatg cccagccgga accaaagcgg gaagctgccg
    26341 ctttctcaaa ttctgctttg aagttatcaa cggagccgaa gtcacgttcg atagccgctt
    26401 tcaggtcacc ctgtaaggtg gtgccttttt tcagaccttt ccagaacagg ctgtggttag
    26461 cgtgaccgcc agcgttgttg cgcagtacgg ttttcttgtc tgctggcagc tggtccagtt
    26521 tggtgatcag ctcttcaacc ggcaggttgg caaattctgg caggctttcc agcgccgcgt
    26581 tggcgttgtt gacgtaggtc tgatggtgtt tggtgtggtg gatttccatg gtctgcttat
    26641 cgaagtgcgg ttccagggca tcgtaagcat acggcaggga tggcagggta tagctcatat
    26701 tcatctccag tattgtcggg cggccgattg ttaatgcgcg taagcagttg gttcattata
    26761 gttaattaaa tgatattgaa aatgattatc aatgccgtac ttttcgtaag ggtatggttt
    26821 tgcaggaaaa ttcccgagat gtgaagcaaa tcacccactt aatgccgtga ttgccagtaa
    26881 atcgacaacg gcggcaacag gcgaaaggtt aatcgacagc acgattttta cactcatctc
    26941 gtcggagatg tgacgcgacg aaaaatgatg aggataagaa gatgagtaac gcgattacga
    27001 tggggatatt ttggcatttg atcggcgcgg ccagtgcagc ctgtttttac gctccgttca
    27061 aaaaagtaaa aaaatggtca tgggaaacca tgtggtcagt tggtgggatt gtttcgtgga
    27121 ttattctgcc gtgggccatc agcgccctgt tactgccgaa tttctgggcg tattacagct
    27181 cgtttagtct ctctacgctg ctgcctgttt ttctgttcgg cgctatgtgg gggatcggta
    27241 atatcaacta cggcctgacc atgcgttatc tcggcatgtc gatgggaatt ggcatcgcca
    27301 ttggcattac gttgattgtc ggtacgctga tgacgccaat tatcaacggc aatttcgatg
    27361 ttctgcttaa taccgaaggc ggacgcatga cgttgctcgg cgttctggtg gcgctgattg
    27421 gtgtagggat tgtgactcgc gccgggcagt tgaaagagcg caaaatgggc attaaagccg
    27481 aagagttcaa cctgaaaaaa gggctggtgc tggcggtgat gtgcggcatt ttctctgccg
    27541 gtatgtcttt tgcgatgaac gccgcaaaac cgatgcatga agccgctgcc gcactgggcg
    27601 tcgatccact gtatgtcgct ctgccaagct atgtcatcat catgggcggc ggcgcgatca
    27661 tcaacctcgg tttctgcttc attcgtctgg caaaagtgaa ggatttgtcg ctaaaagccg
    27721 acttctcgct ggcaaaaccg ctgatcattc acaatgtgtt actctcagca ctgggcgggt
    27781 tgatgtggta tctgcaattc tttttctatg cctggggcca cgcccgcatt ccggcgcagt
    27841 atgactacat cagttggatg ctgcatatga gtttctatgt attgtgcggc ggtatcgtcg
    27901 ggctggtgct gaaagagtgg aacaatgcag gtcgccgtcc ggtaacggtg ttgagcctcg
    27961 gttgtgtggt gattattgtc gccgccaaca tcgtcggcat cggcatggcg aattaatctt
    28021 tctgcgaatt gagatgacgc cactggctgg gcgtcatccc ggtttcccgg gtaaacacca
    28081 ccgaaaaata gttactatct tcaaagccac attcggtcga aatatcactg attaacaggc
    28141 ggctatgctg gagaagatat tgcgcatgac acactctgac ctgccgcaga tattgattga
    28201 tggtcattcc agtctgctgg cgaaattgct gacgcaaaac gcgctcactg cacgatgcct
    28261 catcacaaaa tttatccagc gcaaagggac ttttcaggct agccgccagc cgggtaatca
    28321 gcttatccag caacgtttcg ctggatgttg gcggcaacga atcactggtg taacgatggc
    28381 gattcagcaa catcaccaac tgcccgaaca gcaactcagc catttcgtta gcaaacgaca
    28441 catgctgact actttcatgc tcaagctgac cgataacctg ccgcgcctgc gccatcccca
    28501 cgctacctaa gcgccagtgt ggttgccctg cgctggcgct aaatcccgga atcgctccct
    28561 gccagtcaag attcagcttc agccgctccg ggcaataaat aatattctgc aaaaccagat
    28621 cgttaacgga agcgtaggag tgtttatcgt cagcatgaat gtaaaagaga tcgccacggg
    28681 taatgcgata agggcgatcg ttgagtacat gcagaccatt accgcgccag acaatcacca
    28741 gctcacaaaa atcatgtgta tgttcggcaa aaacatcttg cggataacgg tcagccacag
    28801 cgactgcctg ctggtcgctg gcaaaaaaat catctttgag aagttttaac tgatgcgcca
    28861 ccgtggctac ctcggccaga gaacgaagtt gattattcgc aatatggcgt acaaatacgt
    28921 tgagaagatt cgcgttattg cagaaagcca tcccgtccct ggcgaatatc acgcggtgac
    28981 cagttaaact ctcggcgaaa aagcgtcgaa aagtggttac tgtcgctgaa tccgcagcga
    29041 taggcgatgt cagtaacgct ggcctcgctg tgacgtagca gatgtcgggc cttcatcagt
    29101 cgcaggcggt tcaggtaacg ctgaggcgtc agtcccgttt gctgcttaag ctgccgatgt
    29161 agcgtacgca gtgaaagaga aaattgatcc gccacggcat cccaattcac ctcatcggca
    29221 aaatggtcct ccagccaggc cagaagcaag ttgagacgtg atgcgctgtt ttccaggttc
    29281 tcctgcaaac tgcttttacg cagcaagagc agtaattgca taaacaagat ctcgcgactg
    29341 gcggtcgagg gtaaatcatt ttccccttcc tgctgttcca tctgtgcaac cagctgtcgc
    29401 acctgctgca atacgctgtg gttaacgcgc cagtgagacg gatactgccc atccagttct
    29461 tgtggcagca actgattcag cccggcgaga aactgaaatc gatccggcga gcgatacagc
    29521 acattggtca gacacagatt atcggtatgt tcatacagat gccgatcatg atcgcgtacg
    29581 aaacagaccg tgccaccggt gatggtatag ggctgcccat taaacacatg aatacccgtg
    29641 ccatgttcga caatcacaat ttcatgaaaa tcatgatgat gttcaggaaa atccgcctgc
    29701 gggagccggg gttctatcgc cacggacgcg ttaccagacg gaaaaaaatc cacactatgt
    29761 aatacggtca tactggcctc ctgatgtcgt caacacggcg aaatagtaat cacgaggtca
    29821 ggttcttacc ttaaattttc gacggaaaac cacgtaaaaa acgtcgattt ttcaagatac
    29881 agcgtgaatt ttcaggaaat gcggtgagca tcacatcacc acaatgaagc aaattgtgaa
    29941 catcatcacg ttcatctttc cctggttgcc aatggcccat tttcctgtca gtaacgagaa
    30001 ggtcacgaat tcaggcgctt tttagactgg tcgtaatgaa attcagcagg atcacattat
    30061 gacctttcgc aattgtgtcg ccgtcgatct cggcgcatcc agtgggcgcg tgatgctggc
    30121 gcgttacgag cgtgaatgcc gcagcctgac gctgcgcgaa atccatcgtt ttaacaatgg
    30181 cctgcatagc cagaacggtt atgtcacctg gaatgtggat agcctggaaa gtgccattcg
    30241 ccttggatta aacaaggtgt gcgaggaagg gattcgtatc gatagcattg ggattgatac
    30301 ctggggcgtg gactttgtgc tgctcgacca acagggtcag cgtgtggacc tgcccgttgc
    30361 ttatcgcgat agccgctcca atggcctaat ggcgcaggca cagcaacaac tcggcaaacg
    30421 cgatatttat caacgtagcg gcatccagtt tctgcccttc aatacgcttt atcaactgcg
    30481 tgcgctgacg gagcaacaac ctgaacttat tccacacatt gctcacgctc tgctgatgcc
    30541 ggattacttc agctatcgcc tgaccggcaa gatgaactgg gaatatacca atgccaccac
    30601 cacacaactg gtcaatatca atagcgacga ctgggacgag tcgctactgg cgtggagcgg
    30661 ggccaacaaa gcctggtttg gtcgcccgac gcatccgggt aatgtcattg ggcactggat
    30721 ttgcccgcag ggtaatgaga ttccggtggt cgccgttgcc agccatgata ccgccagcgc
    30781 ggttatcgcc tcgccgttaa acggttcacg cgctgcttat ctctcttctg gcacctggtc
    30841 attgatgggc ttcgaaagcc agacgccatt taccaatgac acggcgctgg cagccaacat
    30901 aaccaatgaa ggcggggcgg aaggtcgcta tcgggtgctg aaaaatatta tgggcttatg
    30961 gctgcttcag cgagtgcttc aggagcggca aatcaacgat ctcccggcgc ttatcgccgc
    31021 gacacaggca cttccggcct gccgcttcat catcaatccc aatgacgatc gctttattaa
    31081 tcctgaggcg atgtgcagcg aaattcaggc tgcgtgtcgg gaaacggcgc aaccgatccc
    31141 ggaaagtgat gctgaactgg cgcagctacg cggtgaagat ttctcgcaac tgcatattgt
    31201 cggcggcggc tgccagaaca cgctgctcaa ccagctatgt gccgatgcct gcggtattcg
    31261 ggtgatcgcc gggcctgttg aagcctcgac gctcggcaat atcggcatcc agttaatgac
    31321 gctggatgaa ctcaacaatg tggatgattt ccgtcaggtc gtcagcacca ccgcgaatct
    31381 gaccaccttt acccctaatc ctgacagtga aattgcccac tatgtggcgc agcttcactc
    31441 tacacgacag acaaaggagc tttgcgcatg accactcaac tggaacaggc ctgggagctg
    31501 gcgaaacagc gtttcgcggc ggtggggatt gatgtcgagg aggcgctgcg ccaacttgat
    31561 cgtttacccg tttcaatgca ctgctggcag ggcgatgatg tttccggttt tgaaaacccg
    31621 gaaggttcgc tgaccggggg gattcaggcc acaggcaatt atccgggcaa agcgcgtaat
    31681 gccagtgagc tacgtgccga tctggaacag gctatgcggc tgattccggg gccgaaacgg
    31741 cttaatttac atgccatcta tctggaatca gatacgccag tctcgcgcga ccagatcaaa
    31801 ccagagcact tcaaaaactg gggtgaatgg gcgaaagcca atcagctcgg tctggatttt
    31861 aacccctcct gcttttcgca tccgctaagc gccgatggct ttacgctctc gcatcccgac
    31921 gacagcattc gccagttctg gattgatcac tgcaaagcca gccgtcgcgt ttcggcctat
    31981 tttggcgagc aactcggcac accatcggtg atgaacatct ggatcccgga tggtatgaaa
    32041 gatatcaccg ttgaccgtct cgccccgcgt cagcgtctgc tggcagcact ggatgaggtg
    32101 atcagcaaga agctaaaccc tgcgcaccat atcgacgccg ttgagagcaa attgtttggc
    32161 attggcgcag agagctacac ggttggctcc aatgagtttt acatggggta tgccaccagc
    32221 cgccagactg cgctgtgcct agacgccggg cacttccacc cgactgaagt gatttccgac
    32281 aagatttccg ccgccatgct gtatgtgccg cagttgctgc tgcacgtcag ccgtccggtt
    32341 cgctgggact gcgatcacgt agtgctgctg gatgatgaaa cccaggcaat tgccagtgag
    32401 attgtgcgtc acgatctgtt tgaccgggtg catatcggcc ttgacttctt cgatgcctct
    32461 atcaaccgca ttgccgcgtg ggtcattggt acacgcaata tgaaaaaagc cctgctgcgt
    32521 gcgttgctgg aacctaccgc tgagctgcgc aagctggaag cggcgggcga ttacactgcg
    32581 cgtctggcac tgctggaaga gcagaaatcg ttgccgtggc aggcggtctg ggaaatgtat
    32641 tgccaacgtc acgatacgcc agcaggtagc gaatggctgg agagcgtgcg ggcttatgaa
    32701 aaagaaattt tgagccagcg tgggtaaaca ctgccggatg cggcgcgagc gccttatccg
    32761 gcctacgggt cggcaacagt tgtaggcctg ataagacgcg acagcgtcgc atcaggcatt
    32821 gattgccgga tgcggcacga gtgccttatc cggcctacag gtcggcaata gttgtaggcc
    32881 tgataagacg cgacagcgtc gcatcaggca ttgattgccg gatgcggcac gagcgcctta
    32941 tccggcctac gggtcgtgca tctgacaaca ccgaatttac aggaacacag aacatgcaaa
    33001 acattaccca gtcctggttt gtccagggaa tgatcaaagc caccaccgat gcctggctga
    33061 aaggctggga cgaacgcaac ggcggcaacc tgacgctgcg cctggatgac gccgatatcg
    33121 cgccttataa agacaacgtc catgcacaac cgcgctatat cccgctcagc cagcccatgc
    33181 ctttactggc aaatacaccg tttattgtca ccggctccgg caaattcttc cgtaacgtcc
    33241 agctcgatcc tgcagctaac ttaggcgtcg taaaagtcga cagcgatggc gcgggctacc
    33301 atattctctg ggggttaacc aacgaagccg tcccgacatc cgaactcccg gcgcactttc
    33361 tctcccactg cgagcgcatt aaagccacca acggtaaaga tcgggtgatc atgcactgcc
    33421 acgccaccaa cctgatcgcc ctcacctatg tactggaaaa cgacaccgcg gtctttactc
    33481 gccaactgtg ggaaggcagc accgagtgtc tggtggtatt cccggatggc gttggcattc
    33541 tgccgtggat ggtgcccggc acggacgaaa tcggccaggc gaccgcacaa gagatgcaaa
    33601 aacattcgct ggtgttgtgg cccttccacg gcgtcttcgg cagcgggtcg acgttggatg
    33661 aaacattcgg tttaatcgac accgcagaaa aatcagcaca agtattagtg aaggtttatt
    33721 cgatgggcgg catgaaacag accatcagcc gtgaagagtt gatagcgctc ggcaagcgct
    33781 tcggcgttac accactagcc agtgcgctgg cgctgtaagg agcaaacatg atccgcaaag
    33841 cctttgtcat gcaggttaac cccgacgccc acgaagagta tcagcgtcgg cataacccca
    33901 tctggccgga actggaagca gtgttgaaat ctcacggtgc gcataactac gccatttacc
    33961 tcgacaaagc gcgtaatctg ctgtttgcca cggtagagat tgaatctgaa gaacggtgga
    34021 atgcggtagc cagtactgat gtttgccaac gttggtggaa atatatgacc gatgttatgc
    34081 ccgctaaccc ggataacagc ccggtgagta gcgagctg
//
LOCUS       DADLFA010000023        33383 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000023 DADLFA010000000
VERSION     DADLFA010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33383
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.023"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            1267..2046
                     /gene="ipaJ"
                     /locus_tag="JQH65_000939"
     CDS             1267..2046
                     /gene="ipaJ"
                     /locus_tag="JQH65_000939"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HAY6138427.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(2992..3921)
                     /locus_tag="JQH65_000940"
     CDS             complement(2992..3921)
                     /locus_tag="JQH65_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HAY6138428.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(4138..4374)
                     /locus_tag="JQH65_000941"
     CDS             complement(4138..4374)
                     /locus_tag="JQH65_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAY6138429.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESALEHIITIGDLISVVKNSTKSI"
     gene            complement(4378..6279)
                     /gene="ipaA"
                     /locus_tag="JQH65_000942"
     CDS             complement(4378..6279)
                     /gene="ipaA"
                     /locus_tag="JQH65_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HAY6138430.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNDLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKVFNHTPDN
                     SDGIGSDLLTTSSQERSTNNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANTINYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            complement(6288..7286)
                     /gene="ipaD"
                     /locus_tag="JQH65_000943"
     CDS             complement(6288..7286)
                     /gene="ipaD"
                     /locus_tag="JQH65_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HAY6138431.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKDLSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSKK
                     EYPINKDARELLHSAPKEAELDGYEMISHRELWDKIAKSINNINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVKSLKDELIKLKEKYKDKPLYPANNTV
                     SKEQANKWLTELGGTIGKVSEKNGGYVVNINMTPIDNMLKSLDNLGGNSEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            complement(7337..8428)
                     /gene="ipaC"
                     /locus_tag="JQH65_000944"
     CDS             complement(7337..8428)
                     /gene="ipaC"
                     /locus_tag="JQH65_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HAY6138432.1"
                     /translation="MEIQNTKSAPILYTDISTKQTQSSSETQKSQNYQQLAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTITPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAETRLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISEQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKILGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRAYELNTLSAQQKQ
                     NIGRATMETSAVAGNISTSGGRYTSALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDNINQSRNSTASQIAGNIRA"
     gene            complement(8448..10190)
                     /gene="ipaB"
                     /locus_tag="JQH65_000945"
     CDS             complement(8448..10190)
                     /gene="ipaB"
                     /locus_tag="JQH65_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HAY6138433.1"
                     /translation="MHNVSTTTTGLPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LAANKNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELAPDSPENKKLRREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGN
                     VASAVFQNNASKNLADLTLSKYQVEQLSKYIGDAIEKFGQLQEVIAELLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            complement(10196..10663)
                     /gene="ipgC"
                     /locus_tag="JQH65_000946"
     CDS             complement(10196..10663)
                     /gene="ipgC"
                     /locus_tag="JQH65_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocator chaperone
                     IpgC"
                     /protein_id="HAY6138434.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            complement(10721..11347)
                     /gene="ipgB"
                     /locus_tag="JQH65_000947"
     CDS             complement(10721..11347)
                     /gene="ipgB"
                     /locus_tag="JQH65_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein IpgB"
                     /protein_id="HAY6138435.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSHNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTSYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            complement(11363..11752)
                     /gene="ipgA"
                     /locus_tag="JQH65_000948"
     CDS             complement(11363..11752)
                     /gene="ipgA"
                     /locus_tag="JQH65_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HAY6138436.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            complement(11765..13249)
                     /gene="icsB"
                     /locus_tag="JQH65_000949"
     CDS             complement(11765..13249)
                     /gene="icsB"
                     /locus_tag="JQH65_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HAY6138437.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILVAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLANQKSLLLFLKTLASEKSAESAFAAYEAVKNSIQHSF
                     TGKDIKLMLNTAESFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISYNLFGLDE
                     TNMSKFICQKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NNVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMSRVLNELKTGATDKKEV
                     IIEKSIKTIDYYNSLKSPDLGTKLYIHDLLQVNKLLLNNSHSNI"
     gene            13563..15179
                     /locus_tag="JQH65_000950"
     CDS             13563..15179
                     /locus_tag="JQH65_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HAY6138438.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGNQKGVSVISYQRVKNGER
                     NKGVEALNRLYLQNQTSLTGKGLLFARDRAAVFYEAIKLAGGDTSKIKAMMEQLDTYK
                     LGEVDKRNINELNKVISEEIRAQLGIKNKKELQSEIKQIFTNYLNNKNWEPINKNINY
                     NGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESKHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELASAALYSRQELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVKALKELNSKRGEPTKLLIRNSDGLLQEVKVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNGESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEILNKKLQKNDNGEPYKLSQRMALLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYAERIGDPKIWNMVKGYSSFV"
     gene            15189..15551
                     /gene="ipgE"
                     /locus_tag="JQH65_000951"
     CDS             15189..15551
                     /gene="ipgE"
                     /locus_tag="JQH65_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HAY6138439.1"
                     /translation="MEDLADVICRALGIPSIDTDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGSLIARLDLTGINEFEDIYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            15551..16009
                     /gene="ipgF"
                     /locus_tag="JQH65_000952"
     CDS             15551..16009
                     /gene="ipgF"
                     /locus_tag="JQH65_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein IpgF"
                     /protein_id="HAY6138440.1"
                     /translation="MSRFVFIFLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKFAVNVNNNGSKDYGIMQINVFHSKRLREMGYTEEMLISHPCLSVHYAAKLLNEFM
                     LMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYKRYLRIAAESKQNNRRI"
     gene            16018..17133
                     /gene="mxiG"
                     /locus_tag="JQH65_000953"
     CDS             16018..17133
                     /gene="mxiG"
                     /locus_tag="JQH65_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HAY6138441.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLAISKDNICQFSDEKGEQIDINSQFNSFEYDGVS
                     FQLKNMMEDKSRVHILNGMYNKPSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKSGFTKCRYILVSNKEINRIQQYINQRFP
                     YINLYVLNLVSDKAELLVFLSKERNSSKDTELEKLKNALIVEFPYIKNIKFNYLSDHN
                     AREDAKGIFTKVNVQYKEICETNKVTYSVREELTDEKLELINRLISDHKNVYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            17141..17392
                     /locus_tag="JQH65_000954"
     CDS             17141..17392
                     /locus_tag="JQH65_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscF/YscF/HrpA family type III secretion system
                     needle major subunit"
                     /protein_id="HAY6138442.1"
                     /translation="MSVTVPDKDWTLSSLSETFDDGTQTLQGQLTLALNALAENPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            17405..17698
                     /gene="mxiI"
                     /locus_tag="JQH65_000955"
     CDS             17405..17698
                     /gene="mxiI"
                     /locus_tag="JQH65_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HAY6138443.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSHPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            17704..18429
                     /locus_tag="JQH65_000956"
     CDS             17704..18429
                     /locus_tag="JQH65_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HAY6138444.1"
                     /translation="MIRYKCFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEIKSEFLTNEVIYVFLG
                     MAVLVVILLVWAFKTGGFKRNKI"
     gene            18426..18953
                     /locus_tag="JQH65_000957"
     CDS             18426..18953
                     /locus_tag="JQH65_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HAY6138445.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIEWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            18895..19590
                     /locus_tag="JQH65_000958"
     CDS             18895..19590
                     /locus_tag="JQH65_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MxiN"
                     /protein_id="HAY6138446.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKDATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            19583..19990
                     /locus_tag="JQH65_000959"
     CDS             19583..19990
                     /locus_tag="JQH65_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa secretion machinery protein MxiL"
                     /protein_id="HAY6138447.1"
                     /translation="MINQINASNALQQRLNSEEFVNLNERLSSSQSFDENIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            19974..20402
                     /gene="mxiM"
                     /locus_tag="JQH65_000960"
     CDS             19974..20402
                     /gene="mxiM"
                     /locus_tag="JQH65_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HAY6138448.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            20532..21164
                     /gene="mxiE"
                     /locus_tag="JQH65_000961"
     CDS             20532..21164
                     /gene="mxiE"
                     /locus_tag="JQH65_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional activator MxiE"
                     /protein_id="HAY6138449.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            21151..22851
                     /gene="mxiD"
                     /locus_tag="JQH65_000962"
     CDS             21151..22851
                     /gene="mxiD"
                     /locus_tag="JQH65_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HAY6138450.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKMTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAKNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDVAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSQGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIVSIPFLSSIPVIGNVFKYKTRNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            22870..23937
                     /gene="mxiC"
                     /locus_tag="JQH65_000963"
     CDS             22870..23937
                     /gene="mxiC"
                     /locus_tag="JQH65_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HAY6138451.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDQDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVESLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIDVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            23950..26010
                     /gene="mxiA"
                     /locus_tag="JQH65_000964"
                     /gene_synonym="virH"
     CDS             23950..26010
                     /gene="mxiA"
                     /locus_tag="JQH65_000964"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiA"
                     /protein_id="HAY6138452.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKISANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            26023..26424
                     /gene="spaK"
                     /locus_tag="JQH65_000965"
     CDS             26023..26424
                     /gene="spaK"
                     /locus_tag="JQH65_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone SpaK"
                     /protein_id="HAY6138453.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNEVL"
     gene            26428..27720
                     /locus_tag="JQH65_000966"
     CDS             26428..27720
                     /locus_tag="JQH65_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HAY6138454.1"
                     /translation="MSYIKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IIARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGVKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMATAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELLGETIR"
     gene            27813..28151
                     /gene="spaM"
                     /locus_tag="JQH65_000967"
     CDS             27813..28151
                     /gene="spaM"
                     /locus_tag="JQH65_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface presentation of antigens protein SpaM"
                     /protein_id="HAY6138455.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIR"
     gene            28138..29016
                     /gene="spa32"
                     /locus_tag="JQH65_000968"
     CDS             28138..29016
                     /gene="spa32"
                     /locus_tag="JQH65_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HAY6138456.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            29010..29891
                     /gene="spaO"
                     /locus_tag="JQH65_000969"
     CDS             29010..29891
                     /gene="spaO"
                     /locus_tag="JQH65_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein SpaO"
                     /protein_id="HAY6138457.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDLLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESDHTEVSLAL
                     FNYDDINVKVDFILLEKKMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            29896..30546
                     /gene="spaP"
                     /locus_tag="JQH65_000970"
     CDS             29896..30546
                     /gene="spaP"
                     /locus_tag="JQH65_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaP"
                     /protein_id="HAY6138458.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLIEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FIVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINVPA"
     gene            30561..30821
                     /gene="spaQ"
                     /locus_tag="JQH65_000971"
     CDS             30561..30821
                     /gene="spaQ"
                     /locus_tag="JQH65_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HAY6138459.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLTIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            30821..31591
                     /gene="spaR"
                     /locus_tag="JQH65_000972"
     CDS             30821..31591
                     /gene="spaR"
                     /locus_tag="JQH65_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaR"
                     /protein_id="HAY6138460.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            31598..32626
                     /gene="spaS"
                     /locus_tag="JQH65_000973"
     CDS             31598..32626
                     /gene="spaS"
                     /locus_tag="JQH65_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein SpaS"
                     /protein_id="HAY6138461.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGGLFKDIILFFLAFSIFVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            32639..32881
                     /locus_tag="JQH65_000974"
     CDS             32639..32881
                     /locus_tag="JQH65_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6138462.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT        10442 a         4822 c         6660 g        11459 t
ORIGIN      
        1 ttgttgactg cctgtgcggc atctggtatc aaggtggcgt gaagccggtt caggttctca
       61 tgcggaacgt tggaacctat atccccccga taccaaaggc gaaatgtaag tgacaaaatg
      121 caatgaacac gttctccgtt aactgaggag catggtgata aataaaggta aatgcagaag
      181 gtgtagagtc tcgcagattt ctgacatcat agagcctaac attagatgca taacgctaca
      241 gattgtagaa tctctacagg tgaaatatac actggttggc gtttatgtcg caaaacatgt
      301 cattcaggtt catctcacca ataaggatat gagtgaagtg gaggataagt gagtctgctg
      361 tcagagtttt ctggtgtatg tcagtaactg cagaaccatg tctgcttgat atgggagctt
      421 atggaggctc tctgtacaag gttagggagc taacaaagca gggataaaaa gtccgcctga
      481 tgcaggcacg gttcgttaaa tcttttgcca ttggtaacaa aatggtgcga tgaatgttca
      541 ggctatcttt atcttgatgg tggttcagct gtttggtaaa gaaatcgctg taacaataga
      601 agaactgcag gcagtactgg tcccacattg tttgctcagt caactggtga agttccgtag
      661 cgcgtaaatt aatgctttgc atgaaacgtt actggagtgg atgaaagaat acctgcatat
      721 atcattactg cgttggatta ccagtacaac agattgaatg aactggacat gttgatagac
      781 ggcattgaaa agcagcttat aagcatagtg cggcagagtt aattggagag cacttcgcct
      841 tctgagtatt aagtaatcat ggtggtacct gcggatacta tctattcact gtgctcggtc
      901 ggcagcctta ctgaccaagg agttggggga gttgataaca aagttgaaaa aacagctctc
      961 aaccaaggtg gttattgtaa caatagcaaa taagctggtg tgacctgtat cacaataacc
     1021 gattatggac gtaaatacga caaaaatatg tcagtatcag gcggtattaa ttgctgatgc
     1081 tgatggacat taactcttaa taaaagggtg gatgctgaaa ggttgttatg gtgacggacg
     1141 tgatgacgac ggaaaagagt atgaggtttt ggatttaaag ttcgttcggg gaataaggac
     1201 ccggtcagct aattctcata gtagttcgca ttgctggatg caatactgtg ctgcaattac
     1261 tcgtcgatgt cggaacaacg gaagccttgc aaacggggat gtattcatac aggtgttatg
     1321 ttatatgggg ttttattgca gggggctata ccaagagaat atatgatatc tcatcagact
     1381 gatgtgaggg taaatgagaa tagagtaaac gaacaaggtt gttttttggc gaggaagcag
     1441 atgtatgaca attcttgtgg agctgccagt ttactctgtg ctgcaaagga attaggggtt
     1501 gataaaatac cacaatacaa agggtctatg tccgaaatga caaggaagag ttctcttgat
     1561 ttggataatc gttgtgagcg ggatttatac ttaataacta gtggtaatta taatccgaga
     1621 atacacaaag ataatatcgc ggatgccggt tattctatgc ctgataagat agtcatggct
     1681 acgaggcttc ttggattgaa tgcttatgtc gttgaagaaa gtaacatttt ttcgcaagta
     1741 attagtttta tttatcccga tgcaagggac ttattaattg gcatgggatg taatatagta
     1801 catcaacgag atgtgttatc ttctaaccaa agagttttgg aagctgttgc tgtatccttt
     1861 attggagttc ctgtcggcct ccactgggtg ttgtgtagac ctgatggaag ctatatggat
     1921 ccagctgtag gagaaaatta ttcttgtttt tcaactatgg aattaggtgc aagaagaagt
     1981 aattctaact ttataggata tactaaaata ggtatttcca tagttataac taatgaggct
     2041 ttgtaaattg tcgtttttta tctgtgaagg agcttgaata ttttggggcg gggggcataa
     2101 caaatgatat tactgagtac aaatttagct atattgggcg gattagttgt gtgggaagac
     2161 gaaatgtttc tcttgaaata agtgaactcc ctcaaggaat gattgtgtgc ttaaatattt
     2221 aagtgctggt aagtttgaga aggctgacat attttgcaga gcaaacagtg attgacttgg
     2281 atgatatttt tattttagtc actcgttgta attggagtat gctttatttt atttgaaaat
     2341 agatgtattt tatacccgca atttaaattg taagccctat gaggggtatg gatattataa
     2401 gttactcttt gactaagcat aacttttggg gttagaaaaa taaatctctt gttaataatt
     2461 aatatgattt taaaatcaaa cttgtttata attataaaat aatgtaagtc ggtcatttta
     2521 tgggaagatt aggagaataa aacagtaatt atatttgaga aatctgctaa tatattctta
     2581 aagctttttt gatactgtga acgaagtctg ttaatggtgg attttttgtt tatcaccatg
     2641 taaaaaaatt gagtagtaaa agcgttgact gctaatcctc gctgttttta ttgaaacgat
     2701 taaaaatacg ctgaatattt tcggccagtc actcatcttc acataaaatt ttgaccatca
     2761 tgccgcatcc cttagttgca caattcattt tcaatgatat gaatattact aatttcacca
     2821 gataatgttc tcgtggtcag cagggtatga gttttcaccg taattttaag gaaacacagt
     2881 gcgtggggat tgaataacag atagccgacg gaattacccc accggctgaa agtcaagttc
     2941 aacgaagatt tttattatct gaattgggca gtttacatca gtgttcgatg tttatgaaga
     3001 cgatagatgg cgagaaatta tatctcgaat agcttcatct atttctctct gaactgtttt
     3061 gtttatttta gataatgtaa atattagagt tctgtttgtt ttattctctt ttctttttat
     3121 gtaagtccgt ttgtctttag aaataaataa cgagtcaact tttagcgaag ggttttgctt
     3181 tctgtttttg cttttcttta tgatgtttaa aatttctttt ttatatatgt ccgggggcaa
     3241 atttgtatca aggtctttta tttcttcctt taatattggt agtgtagaac taagagattc
     3301 attagccttt tcaagtcctt tataataatt gaataatatc ttgtagtcat taaagttaag
     3361 ttctgacgcg attggaaata gagatattat ttcctgtgga acgcttgctg cctgaaaggc
     3421 acgagtgact ttcgcgcgag acagattctc ttttttggct atgtctttat aggacatccc
     3481 tgatactttc agaaaattaa gaccaatacc aagttctcgg atgctatgct cttttgctgt
     3541 ttgtatatcg tttgctagtt ttctggcatc aagagtagat atatattctt ttgaatatag
     3601 aacttcaaga tctgctcctg catatattgc agatgctctt ctacgagtgc catccagaat
     3661 ttcaattcta ccatcaatct cccttcctat tacagggaag aattgttgta gctttatagt
     3721 ttttatgata tcggctaatg attcttctgt tagggattct tgatcgcgta cattcgtttt
     3781 ttggttgacg aaggttaaat cttggatcct tttatgagga atcttggctt tgataaagga
     3841 aactttatta ccagaatgga gtgtaaactc tttcttttgg ccttccttta tacttaacaa
     3901 gtcgttgcac aaatccacca tatcacaccc tgtttattca tattgattta tattgacaaa
     3961 agttaaatgc agtgtgagca agattgtatg taactgttga tgatagtcaa cggtagtctt
     4021 aaaaattact attttaatag ctggattgat gcaatttatc tttattggat aaaagaataa
     4081 tattcttgtg gagctctgat gtgagagctc catagatgaa aaaatgctta tcaattacta
     4141 tattgatttt gtactatttt tgactacaga aataagatct ccaatagtaa taatgtgttc
     4201 taatgcagat tcatctatac gtagattgaa ctcaaaacta atcatatcca gcatttcaat
     4261 cagagaaaga gaatcggcag aaagatcttc aataaattta gtttcttcag atagtttgct
     4321 gtacgctata ccatagcaaa aggccactat tgataatatt ttttctttta tcatatttta
     4381 atccttattg atattcttta atacttttga tagggaagtg gttacatctt ttgctgcttt
     4441 atatatatta ttgttttcag ctgtaatatc attgttattt tttaaatcag atatatcatc
     4501 tgtaagtaat tctgcagagg tatcgtcaat ttttgacaat accttgctga gggcgctaga
     4561 tacttctttt gccttttcat agatcgcatg atttttatca attgtatctg cgttggtttc
     4621 tgttacgtag tttattgtat ttgcatctgt aacttttaat ttgttgttta ttctgtgttc
     4681 tgttgtggat tttgtaatgt cctttgattt ttcctgtaac tctcgttctt gtgttcctga
     4741 tttagttcca atgagagata taagattact tagggagttt gttacttttt ttgaagcttc
     4801 aaatatcgtt tcccgtgtta aatatgtgtc tccatttctt gataaaaaat cagtctgaag
     4861 tacttttgaa tcagtgtttg agttgaattt attgatttgc gaagctactc tatgagaaag
     4921 agaagcgcta cttttagttg tatctgatga gttatcatta ctgcttgtca ctccttccgg
     4981 gtggagaggg ggggtggttg aagagttctg tatgttcaga ggcctgtgtc cccgagaaag
     5041 agagttattt gtagatcttt cttgactact cgtagttaac aggtctgaac ctattccatc
     5101 cgaattatct ggagtatggt tgaaaacctt ttctaaagct gaattatttg caaatgtctg
     5161 gacagtatct gatatagagt gtggtagaac ggaagttact ttttgaacta gtgctctttg
     5221 tggatttagt aatgagtttc cgttacggga taatagatcg tttgtctgag aagtaggaga
     5281 ctgtgcatca ttttctgggt tctcgtgata gctattgtca tatactctgt tgtcaaaaac
     5341 tctattatcg taagttctat tatcattatt tatatgatag tgaattacag gttgggatgt
     5401 tttctcattg gctccggggg ttggtccgcc atctggcata tcttcgggag gtgtagggag
     5461 gccatcttgg ataggatgcg aatcttttat atcttctaaa atatcattaa ggacttttct
     5521 caacagctca ggaaacacgg ctactgtatt taagtctctt gacagtttac ctataccaaa
     5581 tcctttacta tcaatagtat ttttacgttg attgcgcaaa ctatcaaagg caagttttgc
     5641 ctgtgcgtca actaaatcat ctagtttgag agatgaaaca gtttcattac ttaactttaa
     5701 ttcagcaaga atattattct tgataaatga tgatacttca gtattgagga gtttttgtcc
     5761 caatgataaa gcttcaccag atgtgtgttg ttgtgctaat agtgctagtc tgtgctcaat
     5821 gttttgtcgt aattgatgga gtttcttatt ctttgccgac catggtccaa aggcagaatc
     5881 ttgggataat ttatctttta taattagttc cgaatactgt gcgacaattt tttgaagtcc
     5941 cttttcgttt tcaaaattaa gatcattttg atgtactaag gagtacaaaa catttgaaaa
     6001 ttcgagaatc tcgcttttgg catacgcatt cattgagttg gaataaatgc ttcttaacac
     6061 atcttctttc cctgaggaag aaaataaaaa atcaagacat ttctgattaa aagacgttgc
     6121 aaagttttct ttttcagaca ctgatgctgg tgtttttaga gaagtttgcg aactatggat
     6181 atcggatatt ttgcttttta actcgctgta ttctgttgat gatggtgaag ttgccttata
     6241 taagaatgtt ggcgcttgag tattatttac attatgcatg gcgcacctca gaaatggaga
     6301 aaaagtttat ctgtatctgt acatgagctt attgtactac tcaaaacctt tactaaatta
     6361 tcaaaaatac tattggcatt actgtatttt tgaactaaag tttgaagatt atttttcatt
     6421 gtttcatctt cggcagagaa tccggcattc catgcctgat attttgcatt atctagcaca
     6481 acctcgctat ttccacctag attatctaag ctttttaaca tattgtctat tggggtcatg
     6541 tttatattga caacatatcc cccgtttttt tcagatacct tgccgattgt tccacctaat
     6601 tctgtaagcc atttatttgc ttgttcctta ctaacagtat tatttgctgg atatagcggt
     6661 ttatctttat atttttcctt gagctttatc aattcgtctt taagcgattt gacttgtaat
     6721 ttcacggagt ttccgtcgtt acctccggga gagatccagc cggcaagact ggaaagaaca
     6781 gcgctaaaat cttgatacat ttgagtatat gaactaacgg catgttcata tactttcaga
     6841 tactgttcat taatattatt gattgacttt gcaattttat cccacagttc tctgtgagat
     6901 atcatttcat atccatcaag ctcggcttct ttcggggctg aatgtaataa ttctcttgcg
     6961 tctttattaa ttggatattc tttcttggaa agaatatcca atagttgagc ggatttgttt
     7021 acatccatgc taatctgaga tgaatgtaat gctatttctt ctagcgatgt tttagtcaac
     7081 gttttttgtg agagatcttt ctttaatgcc tgatttgttg ttctgatatt atgaagggtg
     7141 tcgttgagca tagtaaggga acttacagga tgagaactgg tcgttgtttt tatatcagaa
     7201 ttaactgttt cggttgatga accgttggta ttgtttgggc tgaatgatga ggtggaaata
     7261 ctattagtca gagttgttat attcatggtt atttccttat aggaagagcc atatattatt
     7321 atggctcttg gatatattaa gctcgaatgt taccagcaat ctgactggct gtagaattcc
     7381 ttgattggtt gatgttgtca attatattca ataatttttg tattaattga tttgtcgctt
     7441 gggatgcttc tttagatact tgggatgctt cctctgcttg tttactgctg gcctgactga
     7501 ttagttgttc ttcttcttca agagcagatg tataacgccc tcctgatgtg gatatattac
     7561 cagcaacggc tgatgtctcc attgttgcac ggccaatgtt ttgtttttgc tgagcagaaa
     7621 gggtattgag ctcgtaagct cttctctggg tgtcaatttt atcctgcaaa gaaatatcgg
     7681 gagaactaag agaagtttta tgttcagttg acagggatat attatctggt gccagtttat
     7741 ttttacctaa aatttttgtg ctagagttag tttgtggtga ggtgatattt gtatctattt
     7801 gtttatttaa ccctaattta gaacctgcaa gctctttttc aagagattga gcagtggcaa
     7861 ggttctttct taaggctcct ttttgttcgc taatccctga atgcgttttt ttggcaccga
     7921 tacccgttat acctacttgt gtgaccgctc cagtaatgct tgatgatagg gctgctaggc
     7981 cttgccgaac aatgttctct gcagctgatt ttgtagcatc gaacgcaatc aatgacaatt
     8041 gagagcccaa tctagtttct gcagtgcgga gagcagaaag tagaacggct acactaataa
     8101 ttaaagaaac agcattagaa gaaagactgg aaatatccag ggtgttctct ggggtaattg
     8161 tgtgactcat ttctgaaaga tctttcatct ttttgtcagt aaggcgagca atgatttctg
     8221 aatcatgctg aacctgctct gataacttta aaagttgatc atcatttaat gtagttgtta
     8281 atacgggatt tttaccgaca ttaagtggaa tatgtgctgc taactgctga taattttgtg
     8341 atttttgtgt ttcggaagaa ctttgagttt gttttgtaga tatatctgta tataaaatcg
     8401 gggctgattt tgtgttttga atttccataa cattctcctt atttgtatca agcagtagtt
     8461 tgttgcaaaa ttgcttttgc aacatcagtt ctattagcct gagagttgga cattgaggct
     8521 aacagctctg caattacttc ctgcaattgg ccgaattttt ctattgcgtc accgatgtat
     8581 tttgacagtt gttcaacttg atatttcgat aatgtcaggt ctgctagatt tttcgacgcg
     8641 ttgttttgga aaacagcaga agcgacattt cctcccgctt gtgtggcaga gttaacactt
     8701 tctcctaata aaactgcttg gtttaaatgg gtggaaataa tttgtttgga tattacttca
     8761 tcacccgctg caccaagaaa tttatttaat ctggcaacag cattagtgat taaatcgtcc
     8821 agttgagaag aaaaattctt gagaaacttt ggtataaggt ctgtgagggt tttacctatt
     8881 attttgccaa tattctctgc aagttttgct gctgcctgtt taccaacagt ggctacgaga
     8941 acgactgctg caaccaagac aagagcgcct gcgattgccc ccagaataga gccaatcatt
     9001 ttggcttttt tcgagtcgac gcccaagcct tcgagcattt ttgtaaatgc atctgaaagg
     9061 agtttgatta agggttcaat gactgctttc atgatcggat tcagggcttg ttccatgaag
     9121 gaattgccgg tcgctgcttg tactatagca tccgtaacca taagagctaa accaacagct
     9181 gccagtgcta gagatgctcc tccagaaaaa gctgctgcaa caacactaac gatagttaaa
     9241 agtgccccaa gtattttccc aacacaaccc attactctgt tgagttcttc tgctttacgt
     9301 acttcagcag catactcatc agattttctc tccatttcag tttttcttga ttcttggaga
     9361 gactggaata gagccagatc attttttaaa gattcttcat tattttttcc aactagttga
     9421 ataaaggttg ccatcaattg agtaacactg gcaagtccgg ttaatgattt ctgctgggtt
     9481 gatagctgtt cagcagatgc tgtgtttgaa aatgcagaaa aagagtctat ttctttttcg
     9541 agttgcattg atttatctgt aagtttgcta tgaattgaca gagttttctg ctcaatcaat
     9601 gtcctgtctt taactgctgc gtcttttttg atagtgagtt gtatttcttc ccgtcttaat
     9661 tttttatttt ctggtgaatc tggggcgagt tcggataatc ttgtttgaat ttggttaatt
     9721 ttattttcta ggtcttttat tttagaatct gcgtttttta gtttattaat ttgtttttca
     9781 tagtctctgg ttagtccttc agtttcagat agaagagtat taattttatc ggagaattct
     9841 aggttttttt gctgtcttgc ctgttgttgg gacttccagg cagtaatttt atttgttaat
     9901 gcagttaaag atttttcacc aagtatttga ataaggtttc caattaaaag cgttagttgg
     9961 gaacttgcat ttaatgactt tggtgcttta agttcaggga ttaatatatt cgaagttgag
    10021 tgtgcattag ttgtattaat atttttgtta gcagcaagat cagcaattgt aagagaaaat
    10081 aatttgttag ctgcatcatt tgcagcctgg atagtattgt ctccaagctc agtggaagcc
    10141 aatattttgg caagaggcaa accagtggtt gtggtgctta cattatgcat aataattact
    10201 ccttgatatc ctgaattgcg tctaagtatg attgtgcttt tatttttaat ttttcatcat
    10261 tgctgtgttg aattacgagt tcgaagcact ctttggcttt caagggggct ttcaaccgaa
    10321 gctggcattg tccagtatgg aataccggtg tatagtcatt ttttcctaat gcaaaagcga
    10381 cagcataaag gtctgctgct tgttggaact gttcttttat ctgataaata gctgcgagtc
    10441 ccataatgta gtctacattg taaaagtcgt atatacataa aaatctgaag aaaacttcag
    10501 cttcctctat tcttcctttg ttgtaaaagt cataagcata tgaataaatg tcatccatca
    10561 tatcatcagg aattgcatta atatctttca gtgtagcgcc agagttaatt gcatcaatta
    10621 ctgcagtaga gatgctttca ttttcagtga tatttaaaga cataaggtct cctttttgtc
    10681 tgcattactt taataagtat aagatttaat ataaacagat ttaatttgta ttgctttgac
    10741 ggtatacagc ctttagaacc tctgcgctta tttctatttg taaaactttt gttgattcat
    10801 taaatatttt atcgtagacg gaactggata ctagatttgt aagatccttc atttgtgtgt
    10861 taaacaaatc catgtcactg ctataacgtt ttaaaatctt tgctctgagg ccagatgagg
    10921 caatctcgtt tagtgctgta tgactgatag atgtttgtgc tgatttttca tcaagtttta
    10981 tatccgggaa ttttttttca atagctgcaa atatgttttt tttgttatct gaggtcaaaa
    11041 caactccatt gtgtatggcc atttcatcta tacttctgtt tatcattgaa gagacataag
    11101 aggttcgctc ttggctcatc caacaaaata gttgattccc cgaattacta tcttttattt
    11161 tgtctatgac gctgtccctg attttattta tattcaccgg gcatccaaaa ttcttctgag
    11221 gaaagcgttg ttttaagtta aaaacagata atattttctt tacaagctta ttgtgactta
    11281 acgaatcaaa tgatgggcga ggaatcgcga attcaacctg tggaagtatt ttgtttagaa
    11341 tttgcatgat accccctata tgttagttca cttctgaagt gatgtttgca atggttagtt
    11401 taatacaaaa taaaaattcg tacaaagatt caatgagttc atcaggtgaa agatattttt
    11461 tgtagtgttt cttggtcaaa acacattgat gagtatcagg gagaagacag atagaagaac
    11521 cataatctct actcaaagag tagttaagga tgagtatgtg ttctggatta ataatatttt
    11581 ctgtaaaaag ttcatgttca ttaagaagtc cggtcaaaaa gatgtattca tcattatctg
    11641 ttatacaaac aggtatttgt tcttcgagca atataaaggc ttgattttta tcgttaaaat
    11701 caagctttgc acctgttatt tcataaagtt tatcatatag tttgcgacac atttctatgg
    11761 ccttctatat attagaatga gagttattca ataaaagctt attgacctgt aataagtcgt
    11821 gaatgtaaag tttggttcca agatctggcg atttaagaga attgtaataa tcaatagttt
    11881 ttattgactt ttcgataatt acttcttttt tatctgttgc tccggttttt aattcgttta
    11941 atacacgaga catattgctg ggtgctgatt ttactaagta tgagtggcaa gcgttgatga
    12001 ccgcatcaaa gtcttctgaa gatacatttt cagtaggatg tttgtaaagt gaaattgata
    12061 aaggcttgaa tgtttttggg gtgtggataa ggttaatttt attgaaactt ttaagatagc
    12121 tacgtcgtaa ttgagtcaat ctggaaagat caaaatcaga ttcaatatac ttcattatct
    12181 cgttataaga ctgattgaga ctttcaatgc gttgcctaac tttatttgca tacgcgtata
    12241 cattgttagg tgtagcaacc aatttaacgt cagggagtgg aaagtatatt tcagaatttc
    12301 ctgctttaag aacattaagt gccataccag cacagttttc agatttactg attaatttat
    12361 aatttgccaa ttgtctgaaa gcatctgctt tcttttggca tataaatttt gacatgtttg
    12421 tttcatcaag gccaaaaagg ttatagctga ttttaccatc ctttgtttta ttatcacctg
    12481 ataatggaat gtatacttta tcagcactga ccccccaata accatagggg tctttttttt
    12541 gattagctct gggaaaaaat atttttttgt tgttattttg atcttgtttt gaatttaata
    12601 agtttctaac ctcttggcct gcatcaagtc tttcggctgt ttggtctgaa atcatattta
    12661 gtttgtcttg tttataggta tcgacactgt aacctgagcg tttttcaaaa aggtagttta
    12721 tttctgtatc ttttgtaaca ttcccgccgg gataccagct gagataggta tgagttggtt
    12781 cctgtagtaa attgtttttt atactgatac tggtatgccc taaatgggtt atgcctctat
    12841 ttccccagca acgaaacact aaatgtcttt caagattttt ggctgtaccg atgccatgaa
    12901 aactctctgc ggtattcaac attaatttga tgtctttacc tgtaaaagaa tgttgaattg
    12961 aatttttgac tgcttcatag gcggcaaacg cactttctgc agatttttcg ctagctaatg
    13021 tcttgaggaa aagcaataag gatttttgat tcgctagtcg ataatttttt agttgttcat
    13081 ttaatatctg tgaatttatc tttgcattaa tagtgcttaa tgttctgaaa aagagatcct
    13141 ttaggttgaa tttctgtgct accaatatag gtccatgctc tgtatcttca acgcgtatag
    13201 gcccttttgt attgcttgcg tcaatgaaat tgctaatttt gagaatcata ctttattaac
    13261 tctccattac gtggtgattg tggggatgta aaatgtattt gatatataat atattcaatt
    13321 aataaattta gaaacttgag cctgttaaca taatcaaatt ttcttttgct gtacataata
    13381 tgtacctcgt gagcatatgt agtgctggtt tcatcatgaa atcccacaag ttaaagtgtc
    13441 tgatatatca ggctcggagt gttatagaaa aagagagaac cctgttgaat aagtatcttt
    13501 tttgttgtaa aacgaggtac aaaactgcaa tcaatgcccc atttatgact gaggataatt
    13561 aaatgcacat aactaatttg ggattgcatc aggtttcatt tcaaagcgga gattcctata
    13621 aaggcgcaga agaaaccgga aaccaaaaag gtgtaagcgt gatttcatat caaagagtta
    13681 aaaatggaga gagaaataag ggggtcgaag cgcttaatcg attatattta cagaaccaaa
    13741 caagcttaac agggaaaggc cttttgtttg cgcgtgacag agctgcagtt ttttacgagg
    13801 caataaagtt ggcaggtggt gatacgtcaa aaattaaagc catgatggaa caattagata
    13861 cctataaact tggtgaagtt gataaaagaa atattaatga gcttaataaa gtaataagtg
    13921 aagagataag agcacagcta ggcattaaaa ataaaaagga attgcaatcg gaaataaaac
    13981 aaatatttac taattattta aataataaga attgggaacc gatcaacaaa aatattaatt
    14041 ataatgggaa aaattatggt tttcaattaa cacccgcctc tcatatgaag atagggaata
    14101 aaaatatatt tgtcaaagag tataatggaa aaggaatttg ttgtgcttct acgagagaga
    14161 gtaagcatat tgcgaatatg tggctttcaa aagtggtgga tgacgagggg aaggaaattt
    14221 tttcgggtat cagacatgga gttatatcag cctatggatt gaaaaaaaac tcatctgaaa
    14281 gagctgttgc agctcgtaat aaagctgagg agttagcaag cgcagcatta tatagcagac
    14341 aagaattatt atcacaggct ttgtctggta aaacagtaga tttaaagatt gtttcgactt
    14401 ctcttctgac gccgaccagt ttaactgggg gagaggagag tatgttaaaa gatcaggtaa
    14461 aggcattaaa agagttaaac tctaaaaggg gggaaccaac gaaattatta attcggaata
    14521 gtgatggtct tctgcaagaa gtaaaggtta atctaaaagt ggtgacattt aactttggtg
    14581 taaatgaatt agcgctaaaa atgggcttag gctggaggaa tgttgacaag cttaatggtg
    14641 aatcaatatg ttctttgttg ggggataatt tcctcaaaaa tggtgtgatt ggaggttggg
    14701 ctgcagaagc tatagaaaaa aatccaccat gtaagaatga tgtgatatat ctggctaacc
    14761 agataaaaga gattctaaac aagaagttac aaaaaaatga taatggagag ccgtataaat
    14821 tgtcacaaag aatggctcta ttggcttata ctataggtgc tgtaccttgc tggaattgta
    14881 agagtgggaa ggacagaaca ggtatgcagg atgctgaaat taaaagagaa ataataagaa
    14941 aacatgaaac agggcagttt tctcaattaa atagtaaatt atcctcagaa gagaaaagat
    15001 tattttctac tattctaatg aatagtggta atatggaaat ccaagagatg aatactggtg
    15061 tgcctggaaa taaagttatg aaaaaattac cgctatcttc gttagagctg tcttatgctg
    15121 aaagaatagg ggaccccaaa atatggaata tggtgaaagg gtattcgtca tttgtataag
    15181 aggaatatat ggaagattta gcagatgtta tttgccgagc cttgggtatc ccttcaattg
    15241 ataccgatga tcaagcaatc atgcttgatg atgatgtgtt aatatatatt gaaaaagaag
    15301 gagattcaat aaatttatta tgcccttttt gtgcattgcc tgaaaatata aatgatctta
    15361 tatatgcgtt gagcctaaat tactcagaaa agatatgttt agctactgat gatgaaggtg
    15421 gaagtctaat tgcgcgatta gatcttaccg ggataaatga attcgaggat atatatgtta
    15481 atacagaata ttatatttcg cgtgtgcgtt ggctgaagga tgaatttgcg cgaagaatga
    15541 aggggtatta atgtcccgtt tcgtatttat ttttctgtgt tttatcccat atttagggag
    15601 agctgattgt tgggataagg ctggtgaaag gtacaacatt ccatcgagtt tgttaaaagc
    15661 gattgcggaa aaagaatccg gatttaataa atttgcagtc aatgttaata acaatggaag
    15721 taaagattat ggtataatgc aaataaatgt ctttcattct aaaagactta gagaaatggg
    15781 atatactgag gaaatgctaa ttagtcatcc atgtctttct gtacattatg cagcaaagtt
    15841 attaaatgag tttatgctga tgtatggaag agggtgggag gctgttggtg cctataatgc
    15901 aggaacatct ccgaaaaaga aaaaagaaag attaaagtac gcagaggata tctataaacg
    15961 ctatttgcgg atagcagcag agagcaagca gaataatcga aggatataag aggatttatg
    16021 tctgaggcaa agaactcaaa tcttgcacca ttcaggttat tagtgaaact cacaaacggt
    16081 gtcggggatg agtttccgtt atattatggg aataacttaa ttgttctcgg acggacgata
    16141 gaaacacttg agtttggaaa cgataatttc ccagaaaata ttattccagt aacagatagt
    16201 aaatcggatg gtataattta tttggccata agtaaagata atatctgtca atttagtgat
    16261 gaaaaaggtg aacaaattga tatcaattca caatttaact catttgaata tgacggagtt
    16321 tcttttcaac ttaaaaatat gatggaggat aaatcgagag ttcatatact aaacggaatg
    16381 tataataaac cctcggtgtt ttttttcttt gctgttattg ttgtgttaat tataattttt
    16441 tccctctctt taaagaaaga tgaagtaaaa gaaattgctg aaattataga tgataagaga
    16501 tatggaatag tcaacacagg acagtgcaat tatattcttg ccgaaacaca aaatgatgcg
    16561 gtatgggcat ctgtagcctt aaataaaagc ggatttacca aatgcagata tattctggtg
    16621 tccaacaaag aaataaatag aattcaacaa tatatcaatc aacgattccc atatatcaat
    16681 ttgtatgtgt tgaatttagt gagtgataaa gcggaattac tggtattttt aagtaaagaa
    16741 agaaatagtt caaaagatac cgaacttgag aaattgaaaa atgctctgat agttgaattt
    16801 ccgtatatca aaaatattaa attcaactat cttagcgatc ataatgcaag ggaggatgct
    16861 aaaggtattt tcacaaaggt aaatgtccag tataaagaga tttgtgaaac taataaagtt
    16921 acatattctg tcagagaaga actgacagat gaaaagcttg agctcattaa tagactgatt
    16981 tctgatcata aaaatgtata tggagatcag tatattgagt tttctgtact tttgatagat
    17041 gatgatttta aaggtaaatc atatcttaac agcaaagaca gttatgtaat gttgaatgat
    17101 aaacactggt tttttttaga taaaaataag tgaggataaa atgagtgtta cagtaccgga
    17161 taaagattgg actctgagtt cattatctga aacttttgat gatggaactc aaacattaca
    17221 agggcaacta acattggcac taaatgcatt agctgaaaat ccttcgaatc cacagttgct
    17281 ggctgaatac caaagtaaat tatctgaata tacattatat aggaacgcgc aatctaatac
    17341 tgtgaaagtg attaaggatg ttgatgctgc aattattcaa aacttcagat aatagggagc
    17401 attcatgaat tacatttatc cagtcaatca ggttgatatt atcaaagcca gtgattttca
    17461 atctcaagag atatcaagcc tggaagacgt cgtgtcggct aaatatagtg atattaagat
    17521 ggatacagat attcaagtat cacaaataat ggagatggta agccatccag aatcattaaa
    17581 cccagaatct ttggccaagt tacagacgac gctctcaaat tattcaatag gagtatcatt
    17641 agctggcacg ttagcaagaa aaacagtttc ggctgttgaa actttattaa agtcttaatt
    17701 tatatgatta ggtataaatg ttttatttta ttcttgttgc tgatgttgat tggatgtgag
    17761 caacgtgaag agttaatttc taatttatct caaagacagg caaatgaaat aatatctgtg
    17821 ctagaacgcc ataatattac tgctagaaaa gttgatggag gtaaacaggg gatctcggta
    17881 caagtcgaaa aggggacatt tgcatcggca gttgatttga tgcgcatgta cgatttgcca
    17941 aatccggaga gagttgatat ctcacaaatg tttcctacag attcattagt gtcttctcca
    18001 agagctgaaa aggcccgttt atatagtgct attgagcaac ggctggaaca gtctttagtt
    18061 tctattggtg gtgttatttc ggcaaaaata catgttagct atgatcttga agaaaaaaat
    18121 atatcttcaa aaccgatgca tatatcagta atcgctatat atgactcacc gaaagagtct
    18181 gaactattag ttagtaatat taagcgattt ttgaaaaaca ccttttctga tgttaagtat
    18241 gaaaatatat ctgtcatatt aactccgaaa gaagaatatg tttatacaaa tgtacaacct
    18301 gttaaggaaa ttaaatcgga atttttaaca aatgaagtaa tatatgtatt tctcgggatg
    18361 gctgtactag ttgtcattct tttggtatgg gcattcaaaa caggggggtt caagagaaac
    18421 aaaatatgat aagaatggat ggaatttata aaaaatatct ttcaataatt tttgatccag
    18481 cgttctatat aaatagaaat cggttgaatt tgccttctga actgttagaa aatggcgtaa
    18541 tcagaagtga gattaataat ctcataatta acaaatatga tctaaattgc gatattgaac
    18601 ctttaagcgg ggtaaccgct atgtttgttg ccaactggaa tttacttcca gctgttgctt
    18661 attttatcgg tagtcaagag tctcggttaa ttaatcatag tgagatggtt attagttatt
    18721 atgggggcaa aatcagtaag caaggggagg ccgctattcg aagtggtttt tggcatctta
    18781 ttgagtggaa ggaaaatata tctgttggta tatatgaaag aataaactta ttatttaatc
    18841 caattgcttt ggagggaaat tatacgcctg tagaaagaaa tttgtcccga ttaaatgaag
    18901 gtatgcaata tgcaaaaagg cactttaccg gtatccagac atcatgccta tgatggtgtt
    18961 gttataaaga gaattgaaaa ggaattatgt aaaaccataa aagatagaga cactgagagt
    19021 aagaagaaag caatttgtgt tataaaggat gctacgaaaa aagctgaatc actgcgtata
    19081 gatgctgtat gcgatggtta tcaaattggt atacagactg cttttgagca tattattgac
    19141 tatatttgtg aatggaagtt aaagcaaaat gaaaacagaa gaaatattga ggactatata
    19201 actagtttgc tttcagaaaa tttacatgat gagaggatca ttagcacttt attggagcag
    19261 tggcttagtt cgttgcgtaa tacagtaaca gaattaaaag tggttttgcc caagtgtaat
    19321 ttagcactga ggaaaaagtt agaacttgat ctccataaat atcgctcaga tgtcaagatt
    19381 attttaaaat attctgaagg gaataattat atattctgta gtggtaatca agtagtggag
    19441 tttagtcctc aggatgtaat atcaggtgtt aaaatagaac ttgcggaaaa attaacaaaa
    19501 aatgataaaa aatatttcaa agagttagca cataagaaac tgagacaaat tgcagaagac
    19561 ttgttaaaag agaatcctgt taatgattaa tcaaataaat gcaagcaatg ctctgcagca
    19621 aagattaaat agtgaagaat ttgttaattt aaatgagaga ttatcatctt cgcaatcatt
    19681 tgatgagaat ataatctatg aaataatgca atatttctca cagagtgaat taaattccat
    19741 agataatgat gaactgcaca ataagattga acaattattc aattcacgtt ttccttatct
    19801 tacagctgca caaaaaagtt cacttcttaa taaactcata gatgcaaatc aatatgtcga
    19861 tttgcatgag ggattttatg catcattgtc catatataac aatattgatt tctatattaa
    19921 gactacaacc ttcgattcat taattagtgt ctttgaagca gggagagagg cagatgattc
    19981 gacatggtag taataagttg aaaatattta ttttaagtat attgctatta acactgagtg
    20041 ggtgtgcttt aaagtcatca tctaattctg aaaaagaatg gcatattgtt cctgtaagta
    20101 aggattattt ttctattcca aatgatttat tatggtcgtt taatacaacc aataaaagta
    20161 taaatgttta ctctaaatgt attagtggta aggcggttta tagttttaat gcaggtaaat
    20221 tcatgggcaa ctttaatgtt aaggaagtag atgggtgctt catggatgca caaaagatag
    20281 ctatagataa actattttct atgctgaaag acggggttgt tttaaaaggt aataagataa
    20341 atgataccat ccttatagag aaggatgggg aagttaaatt aaaattaatt cgagggatat
    20401 aattgtattg tgagtaaata taaaggtcta aatacaagta atatgtttta catttactct
    20461 agtggacata aaccagttaa cgttgagctt gtaaaagata aagaacgtaa cataattgag
    20521 ctggctccag catggaaggg tttttttttg tccgaaatca aaatataaaa ttcagtgata
    20581 atgtgaacta tcattatcgc tttaacatta atagttgtgc aaaatttctt gctttttggg
    20641 actatttcag tggtgctcta gtagagcata gccatgctga aaaatgtatt catttctatc
    20701 atgagaatga tcttcgggat agttgtaata ccgaatcgat gcttgataag ttaatgttaa
    20761 gattcatctt ttcatctgac cagaatgtaa gtaatgcact ggctatgata cgaatgactg
    20821 agtcatatca tttggttctt tatcttctga gaactattga gaaagaaaag gaggtaagaa
    20881 taaagagtct gacagagcat tatggtgtgt cggaggctta ttttagatct ttatgtagaa
    20941 aagctttagg ggcaaaagtc aaagaacaac tgaacacttg gcggttagta aatggtttat
    21001 tagatgtatt tttgcacaat caaacaatca cttctgctgc catgaataat gggtatgcat
    21061 caacatccca tttttcaaat gagataaaaa cacgactagg cttttctgca agagaattat
    21121 cgaatattac ttttttagtg aaaaaaataa atgaaaaaat ttaatattaa atctttgact
    21181 ctcttgattg tattgttacc attgatagtt aatgctaata atattgactc tcatttatta
    21241 gaacaaaatg acatagcgaa atatgttgct caaagtgata cggtaggatc tttttttgaa
    21301 cgtttttctg cgttattaaa ttatcctatt gttgttagca aacaagctgc aaaaaagaga
    21361 atatctgggg agtttgattt atctaatcca gaagaaatgc ttgaaaaaat gacattactt
    21421 gtgggactca tatggtataa agatggaaat gcattataca tatatgactc tggagaatta
    21481 ataagcaaag ttattttgct ggagaatata tcacttaatt atcttattca gtatctgaag
    21541 gatgctaacc tttatgatca cagatatcct ataagaggaa acattagtga taaaacattc
    21601 tatatatctg gtcctccggc attggttgaa ctggtagcta atacagcaac tttgcttgat
    21661 aagcaggttt cttctattgg gacagataaa gttaattttg gggttataaa attaaaaaat
    21721 acatttgtta gtgacagaac ttataatatg agaggggagg atattgtcat cccaggggtg
    21781 gctactgttg tagaacgttt attaaataat ggtaaggctc tgagtaacag acaagccaaa
    21841 aatgatccga tgccaccctt taatattaca cagaaagtat ctgaggatag taatgacttt
    21901 tcatttagtt cagttaccaa tagttctata ttagaggatg tatcacttat tgcatatcca
    21961 gaaaccaatt ctatattagt caaaggtaat gatcagcaaa tacagattat tcgcgatatt
    22021 atcactcagt tagatgtagc caaacgacat atagaattat cattatggat aatcgatatt
    22081 gataaatctg aattaaataa ccttggcgtt aattggcaag gaacggcttc atttggtgat
    22141 tcgtttggtg catcatttaa tatgagctca tcagcatcga taagcacttt ggacggcaat
    22201 aagtttattg cttcagttat ggcattaaat cagaaaaaga aagcaaatgt agtttcaagg
    22261 ccggtgattt tgacacaaga gaatattccg gctatatttg ataataacag gactttttat
    22321 gtgtcattag ttggtgagcg caattcttcg ttggagcatg ttacgtatgg aacattgata
    22381 aatgttatac ctcgcttttc atctcaggga caaattgaga tgtctttaac gatagaggat
    22441 ggaacaggaa atagtcagtc aaattacaat tataataatg aaaatacatc tgtccttcca
    22501 gaagttggaa ggaccaaaat cagtacaata gcgagagttc cacaaggcaa aagtttgttg
    22561 ataggtggtt atacgcatga aacgaactct aacgaaatag tatctattcc ttttctctcc
    22621 tctattcctg taatcggtaa tgtgttcaag tataaaacaa ggaatatcag taatattgta
    22681 agggtttttc tgattcaacc ccgtgagata aaggagtcaa gttattataa tactgcagaa
    22741 tataagagcc tcatatctga aagagaaata caaaagacta cacaaataat cccatctgaa
    22801 actactttgc tggaagacga aaaatcattg gtttcatact taaattacta actataaagt
    22861 aggtgatgta tgcttgatgt taaaaataca ggagttttta gctctgcatt cattgataag
    22921 ttgaatgcaa tgacaaattc agatgatgga gatgagactg ctgatgcaga gcttgattct
    22981 ggcttggcta atagcaagta tattgactca tctgatgaga tggcttccgc tctttcgtca
    23041 tttataaaca gaagagacct tgagaaactg aaaggaacaa atagtgatag tcaggaacgt
    23101 attttagatg gggaagaaga tgaaattaat cacaagattt ttgatttaaa gagaacttta
    23161 aaagataacc ttcctctaga tcaggatttt atagacagac taaagagata ttttaaagat
    23221 ccaagtgatc aagtcttagc attaagggaa cttttgaatg aaaaagatct tactgctgaa
    23281 caagtcgaat cattaactaa aattattaat gagataatat caggtagtga aaaaagtgtt
    23341 aatgctggaa taaattcagc tatacaggct aaattatttg gcaacaaaat gaaacttgaa
    23401 ccacagcttt tgcgtgcatg ttatcgtggt tttatcatgg ggaacatatc aacaacagat
    23461 cagtatatag aatggcttgg taattttggt tttaatcaca gacatacaat tgtgaatttt
    23521 gtagagcagt cactgattgt agacatggat tctgagaaac cgagctgtaa tgcttatgag
    23581 tttggttttg tgttatctaa attaattgca attaagatga ttagaacttc agatgtaatt
    23641 tttatgaaga aactggaatc ctcaagcttg ctaaaagatg gcagtttaag tgcagagcag
    23701 ctattgctaa ctttattata tatttttcaa tatccaagtg aaagtgagca aattcttact
    23761 tctgttatag acgtatcacg agccagtcat gaggattctg tagtgtatca aacatatcta
    23821 tcttctgtta atgaaagtcc tcatgatata tttaaaagtg aaagtgaaag agaaattgcg
    23881 atcaatattc tacgagagct tgtcacaagt gcatacaaga aagagctttc tagataacag
    23941 gagatgaaag tgatccagtc ttttcttaag caagtaagta ctaagcctga attaattata
    24001 ttagtgctta tggttatgat cattgctatg ttgatcatac cattacctac ataccttgtt
    24061 gattttttaa tagggcttaa tattgtactt gctattctcg tttttatggg atcattttat
    24121 attgaaagaa tattgagttt ttctacattc ccttcggttt tgttgataac gacattattt
    24181 cgtctcgctt tatcaataag tactagtcga ctaattttag ttgatgcgga tgcgggtaaa
    24241 attattacta catttggcca gtttgtcatt ggtgatagtc tggctgtagg ttttgttatt
    24301 ttttcaatag taactgttgt gcagttcatt gttataacaa aaggatctga aagggttgcg
    24361 gaggtagctg ctcgcttttc tcttgacggt atgccgggga aacaaatgag cattgatgcg
    24421 gatttaaagg ctggaattat tgatgccgca ggagctaaag aaagacgcag tattctggaa
    24481 cgtgagagtc aattgtatgg ttcatttgat ggagcaatga agttcattaa aggtgatgca
    24541 attgctggca tcattattat ttttgtcaat ttaataggag gtatttctgt tggtatgagt
    24601 caacatggaa tgtctctatc tggtgcatta tctacttata ccatactaac tattggtgac
    24661 ggattggtct ctcagattcc tgctctgtta atttctataa gtgcaggatt tatcgttaca
    24721 agagttaatg gtgatagcga taatatggga cgtaacataa tgtcccagat atttgggaat
    24781 ccttttgttc tcattgttac atcagctctt gccttggcga tagggatgtt gccaggtttt
    24841 ccgttttttg tttttttcct gatagcagtt actttgacgg ctttattcta ttataaaaag
    24901 gtcgtagaaa aagaaaaaag tctgtctgag tctgattcta gtggctatac tggtacattt
    24961 gatattgata atagccatga ctcatctttg gcaatgatag aaaatctgga tgctattagt
    25021 tcagaaaccg ttcctttaat tttattattt gccgaaaata agataagtgc aaatgatatg
    25081 gaaggtctta ttgaaaggat aagaagtcag ttctttattg attatggtgt caggcttcca
    25141 actattttat atagaacaag caatgagctt aaggtcgatg atattgtttt gttaataaat
    25201 gaggtgcgtg ccgatagttt taatatatac tttgacaaag tgtgtattac agatgaaaat
    25261 ggagatatag atgctctagg tattcctgtt gtttcaactt catataatga acgtgttatt
    25321 tcttgggtag acgtttcata tacagaaaat ctaactaata ttgatgctaa aattaaaagt
    25381 gctcaagatg aattttatca ccagttgtca caagctttat taaacaacat aaatgagatt
    25441 tttggtatac aagaaacaaa aaatatgtta gatcagtttg aaaatcggta tcctgatcta
    25501 ttaaaggaag tcttccgaca tgtgactata cagagaattt ctgaggtatt acaaagattg
    25561 cttggagaaa atatttctgt tcgcaattta aaacttatta tggagtcttt ggcgctttgg
    25621 gctcccagag aaaaagatgt cataacatta gttgaacatg tccgtgcatc actttctagg
    25681 tatatttgta gtaaaatagc tgtttctggt gagattaaag ttgtgatgct ttccggatat
    25741 attgaggatg caataagaaa agggataagg caaacctctg gtggctcttt cttgaatatg
    25801 gatatagagg tttcggatga ggtaatggaa actttagcac atgctttgag agaattgaga
    25861 aatgcaaaaa aaaatttcgt tcttttggta tcagtagata tacgtaggtt tgttaaaaga
    25921 cttatagata acagatttaa gagtatactc gttatatcgt atgctgagat tgatgaagca
    25981 tataccatta atgtattaaa gactatttag tgaggtttaa atatgagtaa cattaattta
    26041 gttcaattag ttagagatag tcttttcacg attggttgtc ctccatcaat tattacagat
    26101 ttagacagcc attcagcgat taccatttct ttggatagta tgcctgctat taatatagct
    26161 cttgtcaatg agcaggttat gttatgggct aatttcgatg caccaagtga tgtaaaactt
    26221 cagtcatctg cttataacat attaaattta atgttaatga actttagtta tagcattaat
    26281 gagctggtag agcttcatcg ttctgatgag tacttacagt taagagttgt tattaaagat
    26341 gactacgttc acgatgggat tgtttttgca gagattctgc atgagtttta tcagaggatg
    26401 gaaatcttaa atgaggtctt ataatcaatg agctatataa aattgctcac tcaattatct
    26461 tttcctaata gaatctcggg gccaatcttg gaaacaagtc ttagcgatgt ttcgattggt
    26521 gagatttgta acattcaggc tggaattgaa agtaatgaaa ttattgcaag agctcaggtt
    26581 gtaggatttc atgatgaaaa aacaatatta agcttgattg gaaattctcg tggactttca
    26641 cggcaaacgt tgattaagcc cactgcccag tttcttcata cgcaagtcgg ccgtggatta
    26701 ttgggagcag tagtcaatcc tttaggggag gttactgata agtttgctgt tacggataac
    26761 agtgaaattc tttatcgacc tgtagataat gctcctccgc tatatagtga aagggctgca
    26821 attgagaagc cttttttaac aggtgttaag gttattgatt ctttactcac gtgtggtgaa
    26881 ggacagcgaa tggggatttt tgcgtcagct ggttgtggca aaacttttct catgaatatg
    26941 ctcattgaac atagtggtgc tgatatatat gttattgggt taattggtga gcgaggtcga
    27001 gaggttacag aaacggttga ttatttgaaa aactctgaga aaaaaagcag gtgtgtttta
    27061 gtatatgcaa cttcggatta ctcttcggtt gatcgttgta atgctgcata tatagccact
    27121 gctatagccg aattttttag gactgaagga cataaagtag cgctttttat tgattcatta
    27181 acaaggtatg ccagagcatt acgtgatgtg gccttagccg ctggagaatc acctgccaga
    27241 agaggctatc cggtttcggt ttttgatagc ttacccagac ttcttgaaag gccaggaaag
    27301 ttaaaggcag gtggctctat tactgcattt tacactgttc ttttggagga tgatgatttt
    27361 gctgatccat tagctgaaga ggtaagatcc attttagatg gacatatata tttgagcaga
    27421 aatctagccc aaaaaggaca atttcctgca attgattcct taaaaagtat aagcagggta
    27481 tttacacagg ttgttgatga aaaacatcgt attatggcca ctgcatttcg ggagctactt
    27541 tctgaaatag aagagctaag gacaattatt gactttggtg aatacaaacc gggggagaat
    27601 gcctctcagg ataaaatcta caacaaaata tctgttgttg agagttttct gaagcaagat
    27661 tatcgactcg gttttactta tgagcagaca atggagcttc ttggtgaaac aattagataa
    27721 ggttttaaaa atcaaagata aatatcaacg gtctgttaaa ttaattgaag cacacatatt
    27781 aacactaaaa aaaaaactct ttgtatcgag atgtggaggc attagataaa aggattatat
    27841 acttcctgca actggagaat gacttggaac ctgtaggcgc tcagagtgtt agtcagcttt
    27901 tcaatacaag acgtaagatc gctatagtaa aaaagcatat tatccaatat cagtctgaac
    27961 gaattcttct aaagggaaga attgaagaaa ttcaaaaaga tattgatgag gcaaatgctt
    28021 ctaaaagaaa actgcttcat aaagagagta aaatatgtaa acggattggc ttgattaaaa
    28081 gaaataactt tgccaaacag ttgatacttg atgagttatc acaagaggat atgaagtatg
    28141 gcattagata atataaacct aaattttagt agtgacaaac agatagaaaa atgtgagaaa
    28201 ctatcttcaa tagataatat tgatagtctc gttttgaaga agaagaggaa ggttgaaatt
    28261 cctgagtact ctttaattgc atctaactat tttactattg ataagcactt tgaacataag
    28321 catgataaag gagaaattta tagtggcatt aaaaatgcgt tcgaacttag aaacgaacga
    28381 gcgacatatt ctgatattcc agaatcaatg gccattaaag aaaatatttt gataccagat
    28441 caagatatca aagcaaggga aaaaataaat atcggcgata tgagggggat cttttcatat
    28501 aataagagtg gaaatgcaga caagaacttc gaaagaagtc atacttcttc tgtaaaccct
    28561 gataatctgc tagaatctga taatagaaat ggtcaaattg gtttaaaaaa tcatagcttg
    28621 tctattgata agaatattgc tgacatcatt tctttactaa atggaagtgt tgctaaatca
    28681 tttgagctgc ctgtaatgaa taaaaatact gcagacataa ccccatccat gtcattgcaa
    28741 gaaaaatcaa tagttgaaaa tgataaaaat gtttttcaaa aaaatagtga aatgacttac
    28801 cactttaaac agtggggggc tggacattct gttagtattt cagtggagtc tggttctttt
    28861 gttctaaaac cgtcagatca atttgtagga aataaacttg acttaatttt gaaacaagat
    28921 gctgagggta attacagatt tgatagcagt caacataata aggggaataa aaataatagt
    28981 acaggatata atgaacagag tgaagaagaa tgctaagaat taaacatttt gacgctaacg
    29041 aaaaactaca gattttatat gcaaagcaac tctgcgagcg tttttcgatt cagacattca
    29101 aaaataaatt tacaggcagt gaaagtttag tcactcttac ttccgtgtgt ggggattggg
    29161 taattcgtat tgatacatta tcttttttga aaaaaaaata cgaggtattt tcaggatttt
    29221 ctacacaaga atctttactg catttatcaa aatgtgtctt tatagagtcg tcatctgtat
    29281 tttcgattcc agaactgtct gataagatta ctttccggat cacgaatgaa atccagtatg
    29341 caactactgg gagtcattta tgctgttttt cctcttcttt aggtattatt tattttgaca
    29401 agatgccggt attacgtaat caagtttctc ttgacttatt gcatcatctt ttagagtttt
    29461 gcttaggttc atctaatgta aggctggcta ctttaaaaag aattcgtact ggtgatataa
    29521 tcatagttca gaaactttat aatttattat tgtgtaatca agttattatt ggggattata
    29581 ttgtgaatga taataatgag gcaaaaatta atctgtcaga aagtaatggt gagtcagatc
    29641 acacagaagt ttctttggca ttattcaatt atgatgatat caatgtaaaa gtggacttta
    29701 tccttttaga aaaaaagatg acaatcaatg aactaaaaat gtatgtagaa aacgaattat
    29761 tcaagtttcc cgatgacata gttaaacatg taaatattaa agtaaatggt tctttggttg
    29821 ggcatgggga acttgtttct attgaggatg gttatggtat cgagattagt tcttggatgg
    29881 taaaggagta atctcatgct gagtgatatg tccctcatcg ctacactttc tttttttacg
    29941 ctattaccat ttttagttgc tgctggtact tgttatataa agttttctat tgtttttgta
    30001 atggtacgaa atgcacttgg tttgcagcag gttccatcaa atatgactct caatggtatt
    30061 gcattgatta tggctttgtt cgtgatgaaa cccattattg aagctggtta tgaaaattat
    30121 ctaaatggcc cgcaaaaatt tgatacaata tcagatattg tccgtttttc tgattctggc
    30181 ttgattgaat acaagcaata cttgaaaaag catacagatc ttgagttggc acgttttttc
    30241 caaaggtctg aagaagaaaa tgctgattta aagagtgcag agaataatga ttattcattg
    30301 ttttccctct tgcctgcata tgcgctaagt gaaattaaag atgcgttcaa aataggtttt
    30361 tatttatatt tgccatttat tgttgttgat cttgttattt caagcatttt acttgcactt
    30421 ggtatgatga tgatgagtcc aatcacaata tcagtaccaa taaaacttgt attatttgtt
    30481 gcgcttgatg ggtgggggat cctgtctaag gccttgattg aacaatatat caatgttcct
    30541 gcttagatga tggaggtaat gtgtctgata tagtttatat gggtaataag gctctttatt
    30601 taatccttat cttttcctta tggccagtag gtatagctac ggttattgga ttaactattg
    30661 gtttattaca gacagtgact caacttcaag agcagacact tccttttggt ataaagctta
    30721 taggtgtctc aatatctttg ctacttcttt ctggatggta tggtgaggtt ttattgtctt
    30781 tttgtcatga aataatgttt ttaattaaga gtggggtttg atggacattt caagctggtt
    30841 cgaaagtatt catgtgtttt taatactcct gaacggcgtt ttttttagat tggctccatt
    30901 atttttcttt cttccatttt taaataacgg tataatttct ccatctatta gaatacctgt
    30961 gatttttctt gttgcatcag gattaattac ttctggtaag gtagacatag gttcttctgt
    31021 ttttgaacat gtttatttcc ttatgttcaa ggaaataatt gttggcctcc ttctctcttt
    31081 ttgcttgtct cttccctttt ggatatttca tgctgttggt agtattattg acaaccagcg
    31141 tggggcaacg cttagtagtt caattgatcc tgccaatggt gttgatacgt ctgaattggc
    31201 aaaatttttc aatctttttt ctgcagttgt atttctatac agtggtggaa tggtctttat
    31261 tttagaatcc atacaattgt cttataatat atgcccgtta ttttctcaat gttctttccg
    31321 tgtctcaaat atcttaacat ttctgacttt attggcaagt caggctgtta ttttagccag
    31381 tcctgttatg atagtattgt tactatcaga agtattactt ggtgtattat cgagatttgc
    31441 tccgcagatg aatgcttttt ccgtatcatt aactattaaa agtttacttg caatatttat
    31501 tattttcatc tgttcttcta ctatttactt ttctaaagtt caatttttcc tcggtgaaca
    31561 taaatttttc acaaatctat ttgttagata aaatattatg gcaaataaaa cagaaaagcc
    31621 gacacctaaa aaactaaagg atgccgcaaa aaaaggacag tcatttaaat ttaaggattt
    31681 aacgacagtt gttattattc tggtagggac atttactata atatcattct tttccttaag
    31741 tgatgtaatg ctcttataca gatatgtaat aattaatgac ttcgaaatta atgagggtaa
    31801 atactttttt gcagtggtta ttgtcttttt taagataatt ggcttcccac tttttttctg
    31861 tgttctttcg gctgtgttgc caacattggt tcaaacaaag tttgttcttg cgactaaagc
    31921 tatcaagatt gatttttcag tattaaaccc tgttaaaggg ttaaaaaaaa tatttagtat
    31981 aaagacaata aaagaatttt tcaaaagcat tctgcttctt attattctag cattaacaac
    32041 ctatttcttt tggattaatg accgaaaaat aattttttct caggtgtttt ctagtgttga
    32101 tggcttatat cttatttggg gggggctatt taaggatata atattatttt tcttagcatt
    32161 ttctattttt gttattattc ttgactttgt gattgagttc attttataca tgaaagatat
    32221 gatgatggat aaacaggaga taaaaagaga atatatagag caagagggac actttgagac
    32281 aaagtcgaga aggcgtgagt tgcatatcga gattctttca gagcagacta aatctgatat
    32341 acgtaattca aaattagtgg taatgaaccc gactcatatt gcaattggta tttattttaa
    32401 tccagaaata gcgcctgcac cttttatttc tctcattgaa actaaccagt gtgccttggc
    32461 tgtcagaaaa tatgcaaatg aagttggtat accaactgtg cgtgatgtga aattagctag
    32521 aaaactatat aaaacacata caaaatatag ttttgttgat tttgaacact tggatgaagt
    32581 cctacgtctt attgtttggc ttgagcaggt tgaaaacact cattaataag taaaggagat
    32641 gtgttatatg ggagttaatt tctgtaataa aataggtatt gatcagtctg aatttgaaat
    32701 agaatcttcc atcattaact ccattgctaa tgaggtattg aacccaatat ctttccttag
    32761 caacaaggat ataataaatg ttttactcag aaagatttca tctgaatgtg acctggttag
    32821 aaaagacatt tatcgttgtg ctcttgagtt ggtcgttgaa aaaacacctg atgatttata
    32881 atcattcata ataactcttt tgttgatata aatatttttg ttgaagatat ttatgtagtc
    32941 acaggatcaa ttgtagaaaa ttgttatgtt tttgttagga actgaactaa ataatatatg
    33001 ataagacagc aaaaacgact gaccataatc cttcttttat taggagttga taagagagac
    33061 tattcatcct gtaatgttaa aactctgtta tatagtattc gcgattatgc aaagtctgta
    33121 aatgatcatg agatactaac cgagagtaat cgtttattat ctcattgcat ttcagatagt
    33181 aacggagctt tctttaaaag tagtaaatac gttcccctta agtacttacg taaaaggaga
    33241 atagcacgaa agataccaaa tgattagcac tttgctagta agcttgctgg taaactatat
    33301 tgaccgttgg gtttgttttg agactgtaat ttaaattgtg taattgcctg tttttgatat
    33361 gtttactcca acaacggaga cag
//
LOCUS       DADLFA010000024        32956 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000024 DADLFA010000000
VERSION     DADLFA010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32956
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.024"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..257
                     /locus_tag="JQH65_000975"
     CDS             <1..257
                     /locus_tag="JQH65_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HAY6138463.1"
                     /translation="LDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNGLSVV
                     MTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            267..1641
                     /gene="gspE"
                     /locus_tag="JQH65_000976"
                     /pseudo
     CDS             267..1641
                     /gene="gspE"
                     /locus_tag="JQH65_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
     gene            1631..2833
                     /gene="hofC"
                     /locus_tag="JQH65_000977"
     CDS             1631..2833
                     /gene="hofC"
                     /locus_tag="JQH65_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HAY6138464.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAGQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGFLLAIANKLLMRRPTWLIVRQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNTGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(2868..3911)
                     /gene="guaC"
                     /locus_tag="JQH65_000978"
     CDS             complement(2868..3911)
                     /gene="guaC"
                     /locus_tag="JQH65_000978"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAY6138465.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSDQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            4136..4756
                     /gene="coaE"
                     /locus_tag="JQH65_000979"
     CDS             4136..4756
                     /gene="coaE"
                     /locus_tag="JQH65_000979"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAY6138466.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            4756..5499
                     /gene="zapD"
                     /locus_tag="JQH65_000980"
     CDS             4756..5499
                     /gene="zapD"
                     /locus_tag="JQH65_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HAY6138467.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDSENGQVPERLDFELACC"
     gene            5509..5706
                     /gene="yacG"
                     /locus_tag="JQH65_000981"
     CDS             5509..5706
                     /gene="yacG"
                     /locus_tag="JQH65_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HAY6138468.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(5813..6202)
                     /gene="mutT"
                     /locus_tag="JQH65_000982"
     CDS             complement(5813..6202)
                     /gene="mutT"
                     /locus_tag="JQH65_000982"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HAY6138469.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLAYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(6262..8967)
                     /gene="secA"
                     /locus_tag="JQH65_000983"
     CDS             complement(6262..8967)
                     /gene="secA"
                     /locus_tag="JQH65_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAY6138470.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(9029..9541)
                     /gene="secM"
                     /locus_tag="JQH65_000984"
     CDS             complement(9029..9541)
                     /gene="secM"
                     /locus_tag="JQH65_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HAY6138471.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNVPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQYAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(9772..10689)
                     /gene="lpxC"
                     /locus_tag="JQH65_000985"
     CDS             complement(9772..10689)
                     /gene="lpxC"
                     /locus_tag="JQH65_000985"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HAY6138472.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFIAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLTFKAPSAVLA"
     gene            complement(10790..11941)
                     /gene="ftsZ"
                     /locus_tag="JQH65_000986"
     CDS             complement(10790..11941)
                     /gene="ftsZ"
                     /locus_tag="JQH65_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAY6138473.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(12002..13264)
                     /gene="ftsA"
                     /locus_tag="JQH65_000987"
     CDS             complement(12002..13264)
                     /gene="ftsA"
                     /locus_tag="JQH65_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017374018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAY6138474.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(13261..14091)
                     /gene="ftsQ"
                     /locus_tag="JQH65_000988"
     CDS             complement(13261..14091)
                     /gene="ftsQ"
                     /locus_tag="JQH65_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HAY6138475.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKETAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQLAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(14093..15013)
                     /locus_tag="JQH65_000989"
     CDS             complement(14093..15013)
                     /locus_tag="JQH65_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAY6138476.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLDASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(15006..16481)
                     /gene="murC"
                     /locus_tag="JQH65_000990"
     CDS             complement(15006..16481)
                     /gene="murC"
                     /locus_tag="JQH65_000990"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAY6138477.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFLGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(16535..17602)
                     /gene="murG"
                     /locus_tag="JQH65_000991"
     CDS             complement(16535..17602)
                     /gene="murG"
                     /locus_tag="JQH65_000991"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAY6138478.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(17599..18843)
                     /gene="ftsW"
                     /locus_tag="JQH65_000992"
     CDS             complement(17599..18843)
                     /gene="ftsW"
                     /locus_tag="JQH65_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HAY6138479.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVVFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(18843..20159)
                     /gene="murD"
                     /locus_tag="JQH65_000993"
     CDS             complement(18843..20159)
                     /gene="murD"
                     /locus_tag="JQH65_000993"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAY6138480.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(20162..21244)
                     /gene="mraY"
                     /locus_tag="JQH65_000994"
     CDS             complement(20162..21244)
                     /gene="mraY"
                     /locus_tag="JQH65_000994"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAY6138481.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(21238..22596)
                     /gene="murF"
                     /locus_tag="JQH65_000995"
     CDS             complement(21238..22596)
                     /gene="murF"
                     /locus_tag="JQH65_000995"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAY6138482.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(22593..24080)
                     /gene="murE"
                     /locus_tag="JQH65_000996"
     CDS             complement(22593..24080)
                     /gene="murE"
                     /locus_tag="JQH65_000996"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HAY6138483.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKV
                     IDVNYHDSGVTIRFSSSWGDGEIESHLIGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTVPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(24067..25833)
                     /locus_tag="JQH65_000997"
     CDS             complement(24067..25833)
                     /locus_tag="JQH65_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HAY6138484.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(25849..26214)
                     /gene="ftsL"
                     /locus_tag="JQH65_000998"
     CDS             complement(25849..26214)
                     /gene="ftsL"
                     /locus_tag="JQH65_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAY6138485.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(26211..27152)
                     /gene="rsmH"
                     /locus_tag="JQH65_000999"
     CDS             complement(26211..27152)
                     /gene="rsmH"
                     /locus_tag="JQH65_000999"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAY6138486.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(27154..27612)
                     /gene="mraZ"
                     /locus_tag="JQH65_001000"
     CDS             complement(27154..27612)
                     /gene="mraZ"
                     /locus_tag="JQH65_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAY6138487.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(28214..29218)
                     /gene="cra"
                     /locus_tag="JQH65_001001"
     CDS             complement(28214..29218)
                     /gene="cra"
                     /locus_tag="JQH65_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HAY6138488.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANAPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHLMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(29300..29386)
                     /locus_tag="JQH65_001002"
     CDS             complement(29300..29386)
                     /locus_tag="JQH65_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HAY6138489.1"
                     /translation="MCNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(29398..29889)
                     /gene="ilvN"
                     /locus_tag="JQH65_001003"
     CDS             complement(29398..29889)
                     /gene="ilvN"
                     /locus_tag="JQH65_001003"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HAY6138490.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGNEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIREVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(29892..31616)
                     /gene="ilvI"
                     /locus_tag="JQH65_001004"
     CDS             complement(29892..31616)
                     /gene="ilvI"
                     /locus_tag="JQH65_001004"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HAY6138491.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVVAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            complement(31934..32878)
                     /gene="leuO"
                     /locus_tag="JQH65_001005"
     CDS             complement(31934..32878)
                     /gene="leuO"
                     /locus_tag="JQH65_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HAY6138492.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT         7911 a         9253 c         8017 g         7775 t
ORIGIN      
        1 gattagatac ctgcgacggt ggcagcaatg gcattccctc gcctaccacc acccgctatg
       61 tttcagccat gagtgtggca aaaggcgtgg tgtcgctgac cgggcaagaa agtctcaatg
      121 gactaagcgt cgtcatgacg ccaggttggg ataacgcaaa cggcgtcacc ggctgggcgc
      181 gcaactgcaa tattcaaagt gacagcgcat tgcagcaagc ctgcgaagat gtcttccgct
      241 ttgatgacgc caactaagga gcggcaatga atattccaca gctcaccgcc ctgtgtctgc
      301 gttatcaggg agtcttgctg gatgccagcg aagaggtggt tcatgttgcg gtagtcgatg
      361 caccttcgca tgagttgctg gacgcattgc atttcgctac caccaaacgt attgagatca
      421 cctgctggac gcgccaacaa atggaaggcc acgccagtcg cacacaacag acattgcccg
      481 tagctgttca ggagaagcat cagtcgaaag cagagttgct gactcgaacg ttacaatctg
      541 cgctggaaca acgtgcgtct gatattcata tcgaaccagc ggacaatgcc taccgcatcc
      601 gcttgcgtat cgacggcgta ttgcatcctt taccggatgt ttcaccggat gccggagtcg
      661 cattaaccgc cagattaaaa gtgctgggaa acctggatat tgcggaacat cgcctgccgc
      721 aggacgggca attcactgtc gaactggcag gaaacgccgt ctcatttcgt attgcgacct
      781 taccatgtcg gggtggtgaa aaggtggtat taaggttgtt acagcaggtg aaccaggcac
      841 tggatgttaa cacgctggga atgcagccgt tacaactggc ggactttgct catgccttgc
      901 aacaaccaca gggactggtg ctggtaactg gccctacagg cagcggcaaa acggtcacgc
      961 tttatagtgc cctgcaaaag ctgaataccg ctgacattaa tatttgtagc gtcgaagatc
     1021 cggttgagat ccccatagcc ggactaaacc agacgcaaat ccatccgcgt gccgggctca
     1081 cctttcaggg cgttttgcgt gcgttattgc gccaggatcc tgacgtcatc atgatccgat
     1141 ggcgaaacgg cagaaattgc cattaaagcc gcgcaaaccg gtcacctggt gttgtctacc
     1201 ctacacacta attccacctg cgaaacgctg gtacgtttac agcaaatggg agtcgcccgc
     1261 tggatgctct catcagcgct tacgctggta atagcccagc gtctggtacg taaattttgc
     1321 ccacattgtc gccagcagca aggggagccc atccatattc cagacaatgt atggccgtcg
     1381 ccgctgcccc actggcaggc acccggttgt gtacattgct accacggttt ttatggtcgt
     1441 accgccttgt ttgaagtcct gcccattaca ccagccatac gccagcttat ttccgctaat
     1501 accgacgttg aatcgctgga aacgcacgca cgacaggcgg gtatgcgtac gctttttgaa
     1561 aacggctgcc tagccgtgga gcaaggctta accacctttg aagagttaat ccgcgtactg
     1621 gggatgccgc atggcgagta agcaactctg gcgctggcat ggcataaccg gcgacggcaa
     1681 tgcgcaagat gggatgctat gggcagagag ccgtgctttg ctgctcatgg cactacagca
     1741 acagatggtt accccactta gcctgaagcg aatcgccatc aattctgcgc agtggcgagg
     1801 agataaaagc gcggaagtca ttcatcaact ggcgacgcta ctcaaagccg ggttaacgct
     1861 ttctgaaggg ctggcactgc tggcgggaca gcatcccagt aagcaatggc aagcgttgct
     1921 gcaatcgctg gcgcacgatc tcgaacaggg cattgctttt tccaatgcct tattaccctg
     1981 gtcagaggta tttccgccac tctatcaggc gatgatccgc acgggtgaac tgaccggtaa
     2041 gctggatgaa tgctgctttg aactggcgcg tcagcaaaaa gcccagcgtc agttgaccga
     2101 caaagtgaaa tcagcgttac gttatcccat catcatttta gcgatggcaa tcatggtggt
     2161 tgtggcaatg ctgcattttg ttctgccgga gtttgccgct atctataaga ccttcaacac
     2221 cccactaccg gcactaacgc aggggatcat gacgctggca gactttagtg gcgaatggag
     2281 ctggctgctg gtgttgttcg gctttctgct ggcgatagcc aataagttgc tgatgcgccg
     2341 accgacctgg cttatagtgc ggcagaaatt gctgttacgc atcccgatta tgggttcact
     2401 gatgcgggga caaaaactca cgcagatctt tacgattctg gcgctgacac aaagtgcagg
     2461 cattactttt ttgcagggcg tagagagcgt cagagaaaca atgcgctgcc cgtactgggt
     2521 gcaacttctg acacaaatcc agcacgatat cagtaacggt catcccatct ggctggcgct
     2581 aaaaaatacc ggggagttta gcccgctctg tttgcaatta gtgagaacag gagaggcatc
     2641 cggctcgctg gacctcatgt tagacaacct cgcccatcat catcgggata acacaatggc
     2701 gctggcggat aacctcgcag ccttactgga accggcgttg ctgatcataa cgggaggaat
     2761 tatcggtacg ctggtggtgg cgatgtatct gccaattttc catttaggcg atgcgatgag
     2821 tgggatggga taacgctggc gtgttgctcc acaccagcgt tggaagatta caggttgttg
     2881 aagatgcggt tttcttgttc ctgcacacgg ataaatgtgg tgcgcttggt cagttctttc
     2941 aggcgtgaag ccccaacgta tgtacaagct gaacgcaggc cgcccaaaat atcacgcgcg
     3001 gtattttcaa ccgggcctcg cagcggcagc ttaacggttt taccttctgc tgcgcgatat
     3061 tccgcaacgc cgccaacgtg acgtttcatc gcagactcgg agctcatgcc gtagaacagc
     3121 ataaatttct cgccgttctc ctcaacgatg cgaccgccgc tctcttcgtg gcccgccagc
     3181 atgccgccaa gcatgacgaa atcggcaccg ccgccaaagg cttttgcgac atcgcccggc
     3241 gtggtgcagc caccgtcgct gacgatcatt ccgcccagac cgtgcgccgc atcggcacat
     3301 tcgattaccg cagaaagctg cggataaccg acgcctgttt tgacgcgagt tgtacaaaca
     3361 gaacctgggc caatgccaac tttaacgata tcagcaccgg agaggataag ctcctcacac
     3421 atttcaccag tcactacgtt gccagcacaa atggttttgg tcggccacgc ttcacgcgct
     3481 ttcgcaacga actgcacgaa gtgttcggaa taaccattcg ccacatcaat acaaacaaag
     3541 tttaatgccg ggttcaggtc gagaatctgt ttagtttttt cgaaatccgc atcagacgta
     3601 ccggtagaaa ccatcacatg tttcagcaca tcagcggaag aattgttgat aaacgcttgc
     3661 cactcttcga cagaatagtg tttatgcaca gcagtcaaaa tatcaaaaga agccagcgca
     3721 gaggccatag aaaatgtacc tacggtgtcc atatttgcgg cgataatcgg cacgccggac
     3781 cagctctgat ctgaatgttt gaaggtgaat tgacgttcca gttcaacatc ggaacggctt
     3841 ttaagagtgg agcgtttagg gcggatgaga acgtctttaa aacctaactt cagatcttct
     3901 tcaatacgca tgtgcggatt cctggggtta atggctataa gggtaagagc gcaactccag
     3961 tgacgctatc atacgcacta atcaatgcgg cgcaagactg cgaaattgtc tttttttacg
     4021 ctacaatccc ataaatttac ctggtaaata gtgagcgaac gtttcatcaa tttcgctttc
     4081 gtgcactcat ttttaacgtt ttgataatca tgatgaattc ccgggaatat aaattatgag
     4141 gtatatagtt gccttaacgg gaggcattgg cagtggcaag agtaccgttg ccaatgcgtt
     4201 tgctgatctc ggaattaacg tcattgatgc cgatattatt gcgcgtcagg tggttgaacc
     4261 aggtgcacct gcgctacatg ccattgctga tcactttggc gctaacatga ttgctgctga
     4321 tggaacattg cagcgccggg ccttgcgcga gcggatcttc gccaacccgg aagagaaaaa
     4381 ctggcttaac gccctgctgc atccgctgat tcagcaagag acgcaacacc agatccagca
     4441 agctacttcc ccctatgtac tgtgggttgt gccattgctg gtagaaaact cactgtataa
     4501 aaaagcgaat cgagtgctgg tggtggatgt cagcccagaa acgcaactta agcgcaccat
     4561 gcagcgcgat gatgtaactc gcgagcatgt cgaacaaatc cttgctgctc aggcaacgcg
     4621 cgaagcccgc cttgccgtgg cagatgacgt cattgataat aacggcgcac cggatgctat
     4681 cgcatcggat gttgcccgcc tgcacgcaca ctatttgcag cttgcgtcgc agtttgtctc
     4741 acaggaaaaa ccgtaatgca gacccaggtc ctttttgaac atccactaaa tgaaaaaatg
     4801 cgtacatggc tgcgcattga gtttttgatt cagcaactca ccgttaattt acccatcgtt
     4861 gaccacgctg gcgcgctgca tttcttccgt aatgtcagtg aattactgga tgttttcgag
     4921 cgcggcgaag tccgcactga gctgttgaaa gaacttgacc ggcagcaacg taaactccag
     4981 acctggattg gcgtgcctgg cgtggaccag agccgtattg aagcattaat tcagcagtta
     5041 aaagcggcgg ggagcgtatt aatttccgcg ccgcgtatcg ggcaatttct gcgtgaagat
     5101 cgtttgattg ctctggtgcg tcagcgactg agcatcccag gcggctgttg cagctttgat
     5161 ttacctacat tgcacatttg gctgcatcta ccccaggcgc agcgcgacag ccaggtagaa
     5221 acctggattg ccagcctgaa cccgctcacc caggcactta ccatggtgct ggatttaatt
     5281 cgccagtcgg cccccttccg taaacaaacc agcctgaatg gtttttatca ggataacggt
     5341 ggcgatgccg acttgctgcg cctgaatctg tcgctcgatt cacagcttta tccgcaaatt
     5401 tccggacata agagccgttt tgccattcgt tttatgccgc tggacagtga aaacggacag
     5461 gtaccggaac gtctagattt cgaactggcc tgttgctaag gagtgaaaat gtcagaaact
     5521 attacggtga attgcccaac ctgcgggaaa acggtggtat ggggtgaaat cagcccgttt
     5581 cggccatttt gctccaaacg ttgtcagctg atcgacctcg gagaatgggc tgctgaagaa
     5641 aaacgaatcc ccagcagtgg cgatctttcc gaaagcgatg actggagcga agagccaaag
     5701 cagtgacatt ggtctgatgc cgcacatagg cctgataaga cgcggacagc gtcgcatcag
     5761 gcatcttgtg caaatgtcgg atgcggcgtg aacgccttat ccgacctaca gactacagac
     5821 gcttaagctt cgcaattacc ggttcattgg ctggcggaaa atcatcggca ttaagaccga
     5881 ccagcgacat ccactcaccg ggttgccctt ctttacccca cggcacccct tcccaacttt
     5941 cgaccagcca gaaccacaga gttatatgcc tgtccgggaa ttcatatgcc agtttttcaa
     6001 atagcgaaaa atgttgtggg gtaatcccga cctcttcctg aagttcacgc accaccgcct
     6061 gttccggcgt ttcgcccatt tcaattttac cgccgggaaa ctccagttta ttcgccatgt
     6121 gcgcatctgc tgcgcgacgc gttataaaga tttcattgtt ctcgttgcga ataataccta
     6181 ccgcaatttg cagctttttc attgttttaa acctataaaa aaggcgcaga atcctgcgcc
     6241 ttttgcttca acagttagct tttattgcag gcggccatga cactgcttgt attttttacc
     6301 agaaccgcac gggcaaggat cgttacgtcc tactttgcgc tcaccggttt gcgccgccag
     6361 tgcagctgcg gctgcagagt cgtcatcctg atggctaagc tgctgcattt gcgctaaacg
     6421 ctcggcttcc atacgacgct gttgttccag ctcctcaacc tcttcaggca tacgtacctg
     6481 aactttgctc agcgtactga taacttcata tttcaacgac tccagcatcg ctgcaaacat
     6541 ggagaacgat tcacgtttgt attcctgctt cggatctttc tgtgcatagc cacgcaggtg
     6601 gataccctga cgcagatagt ccatcgctgc caggtgctct ttccacaggg agtcaagcgt
     6661 ttgcagcatg acgcctttct cgaagtgacg catcatctca gcaccaacca cttcttcttt
     6721 acgctgatac acttcgatgg actgcgccag aatgcgctca cgcagcgtct cttcatgcag
     6781 ttctggttct ttatccagcc actcggcaat tggtaaatcg aggtcgaaat cgttcttcag
     6841 acgttcctgc agccccggaa tatcccacat ttcttccagc gactgtggcg gaatgtaggc
     6901 atcaatggtg gctttgaaca catcttcacg aatgctgttg atggtttcgc tcacatcgct
     6961 gacatccagc agttcgttac gctgggagta aatggcgcga cgctgatcgt tagccacgtc
     7021 atcatattcc agcagttgct tacgaatgtc gaagttacga ctttcaactt tacgctgggc
     7081 gttggcaatc gctttagtca cccacgggtg ttcaatggct tcgcctggct tcatacccag
     7141 tttacgcatc atgccggata ctcggtcgga agcaaaaata cgcatcagcg catcttccat
     7201 cgacaggtag aaacgggaag aaccagcatc cccctgacga ccagaacgac cgcgcaactg
     7261 gttatcgata cgacgggatt cgtgacgctc ggtaccgatg atatgcaggc cacctgcttc
     7321 cagtaccgca tcgtgacgta cctgccagtc ggctttaatt ttttcaattt gctctgcggt
     7381 cggattttcc agcgcggcaa cttctgcctg ccagctacca ccgagcacaa tatctgtacc
     7441 acgacccgcc atattggtcg cgatagtcac cgcagccgga taacctgcct gagcaacaat
     7501 cgccgcttcg ttggcgtgga atttggcgtt caggacgttg tgcttaatac cggctttggt
     7561 cagttcgttt gacaccagct ccgatttttc gatggagata gtacccacca gcaccggctg
     7621 gcctttcgca gtacgttctt tgatatcttc aatgatcgcc tgaatttttt ccgcttcagt
     7681 catgtagacc aggtccggca gatctttacg aatcattgga cggttggtcg gaacaacgac
     7741 ggtatccagc ttgtagatgg agctaaattc gaaagcttcg gtatcagcag taccggtcat
     7801 ccccgccagt ttttcataca gacggaagta gttctggaag gtgatcgaag ccagcgtttg
     7861 gttttcgttc tggatctgca caccttcttt cgcttccaca gcctggtgca gaccatcgga
     7921 ccagcgacgg ccctgcatgg tacgaccggt gtgttcgtca acgatgataa cttcaccatc
     7981 tttaacgatg tagtcgacgt cgcgggtaaa cagcgcatga gcgcgcagcg ccgccgttac
     8041 gtggtgcatc agcatgatgt tggccggaga gtacagagac tccccttcat ccatgatgcc
     8101 ctctttcacc agcagttctt caatcagcac cagaccacgt tcggtcaggt tcacctggcg
     8161 agatttttcg tccaccgaga agtggccttc gccctggaag gtttcggagt cttctttttc
     8221 ctgacggatc aggtgcggaa taattttatt cacgcgttta tacatttccg agctgtcttc
     8281 tgccgggccg gaaatgatca gcggtgtacg cgcttcatcg atcaggatgg agtccacttc
     8341 gtccaccagc gcatagtgca gtttacgctg tacacgttct tcagggctga acgccatatt
     8401 gtcgcgcagg tagtcaaagc cgtattcgtt gttcgtaccg taagtgatgt cagctgcgta
     8461 agcttcgcgc tttgccggtg ctggcatgcc cggcaggttg ataccgacag tcaggccaag
     8521 gaattcaaac agcggacggt tgttttcggc gtcacgttgc gccaggtagt cgttgacggt
     8581 aactacgtgc acgcctttac cggttagtgc gttcaggtaa gcaggcagcg ttgcggtcag
     8641 ggttttacct tcaccggtac gcatttcggc gatgcagcgt tcgttaagaa ccataccgcc
     8701 gagtaactga acgtcgaagt gacgcatacc aaagacgcgc ttacttgcct cacgtaccac
     8761 ggcgaaagct tccgggatca gattttccag cacttcgcct ttttccagac gtgcacgaaa
     8821 ctctgcggtt ttccctttca gttcttcgtc ggagagtttt tccatctccg gttccatggc
     8881 attgatgatg ttgaccactt tgcgcatccg gcgcagggtg cgatcgttac gactaccgaa
     8941 aactttagtt aacaatttga ttagcataat aaaatctcaa acgccccgcg ttgcggagtt
     9001 aataaaatga agtaaaggtt tattgttgtt aggtgaggcg ttgagggcca gcacggatgc
     9061 cttgcgcctg gcttatccag acgggcgtgc tgaattttgc ttgtggggta aaatgcgcat
     9121 aatcaatgcg ataacccttt tcagacggcg tgccttcctg ggtcagcagc gcgctgagcg
     9181 tatccagtaa tgcaagatgt tgcgcctgaa gaggcaaaga ttcttcagca acgggcagtg
     9241 tttgcggtgc cattgcgaaa gaaagatgac ggattaccgt gcgaatggca tattgatgcc
     9301 agtaatcaac ggaatagttc gaattcgggc ggcgtgtgtt cgcttccagc aaggccaatt
     9361 gaccaaagtt aactttggct gaaggctcgt ggttgcgggt tgtcgctttt gcgggcacgt
     9421 ttggttcggc ggcgttgctg agcgcaggca aacctaaact cgccgcgacc atccctaata
     9481 agagatgcgg ccagaagtag cgtttaccaa actgtcgcca gcgcgtcagt attccactca
     9541 cgttattgcc atcccgatcg aattcaaacg tatttagcgc acaaatctta tcattaaagc
     9601 ctgaagtcca cagcatgaat gaagataagt tgtgcgtaaa agtgcttaag aaaacagcgt
     9661 tcgcaccgtt ttttatagct cttataaaat tcaaaaaaaa acgactggtt cacctggccg
     9721 gagagagtgc cagatttgcc agtcgaattt tatacgacag tataaatgtc gttatgccag
     9781 tacagctgaa ggcgctttga aggtcaacgg cagttctgcg tcgtcctgga aggtcacata
     9841 ttcccaagct tcctgtttcg ccaggacagc ctgcagcagt ttgttattca gtgcatgacc
     9901 ggatttataa gcgataaatg caccaataat attgtgacca cacatgaaca agtcaccgat
     9961 cgcatcgagc attttgtgac gcacaaattc gtcttcaaaa cgcaggccgt cttcgttcag
    10021 tacgcgataa tcgtcaacaa cgatggcaca atcgaagctg ccgcccaggc acaaaccacg
    10081 ggactgcaga tattcgatat cacgcatgaa accgaacgta cgcgcacggc tgatctggcg
    10141 cataaacgca tcagcggaga agttcatcgc atagcgctgg ttgctggaat caatagccgg
    10201 atggttaaaa tcgatggtga aatccagcga aaaaccattg tacggcttaa attcagccca
    10261 cttatcgcca tcttcgacac gaacagtctc tttgatgcga acaaattttt tggcgcagtt
    10321 caactcgtcg ataccggcgt caagcagcag gtatacaaac ggagcggcgc tgccgtccat
    10381 gatcgggatt tccggcgcgt taacttcgat aacaatgtta tcgatgccca ggcccgcgag
    10441 agcagcattg aggtgctcta cggttgaaat ccgtacatca tgctcgttga ccagacacgt
    10501 acagagcatg gtatcacgca cagatttggc atcggccggg aaatctaccg gtggattcaa
    10561 gtcggtgcga cgatagatga ccccggtgtt ggccggcgca gggcgtaacg tcagagtgac
    10621 tttcttgccg gtatgtaaac cgacacccgt cgcctgaacg atacgtttaa gtgtcctttg
    10681 tttgatcatc gtattatctc gccaaattac ctatccaacc gaagtgtact atacattcgg
    10741 cgggccagtt tagcacaaag agcctcgaaa ccaaaattcc agtcaattct taatcagctt
    10801 gcttacgcag gaatgctggg atatccagat aatccggctc tttcgcagtt tgcggcgcat
    10861 tgtcattcac gactttagca accggcttct gctcctgggt cagcggagcc atcccatgct
    10921 gctggtagcg atccatcact ggctgctgaa cctgcttatt ggtcaccaga gtgatttcag
    10981 gacgtttgtc catgccgata cctgtcgcaa caacggttac gcgcagctcg tcattcatat
    11041 ccgggtcaag agaagtaccg ataaccacag tcgcgttgtc ggaagcaaat gcacggatgg
    11101 tgttacctac cgtttcgaac tcatccagac gcaggtcgaa gcccgccgtg atgttaacca
    11161 gcacgccgcg cgcgccagac aggtcgatat cttccagcag cggagaagag atagccattt
    11221 cagcagcttc ttccgcacgg tcttcaccgc tcgccacgcc agaacccatc attgcgtagc
    11281 ccatctcaga cattacggtg cgtacgtctg caaagtccac gttcatcaaa cccggacgag
    11341 taatcagttc agcgatacct tgcacagcgc ctttcagtac atcgttcgct gcgccaaacg
    11401 catccagcag ggagataccg cggcccagaa ctttcagcag tttgtcgttc gggatagtga
    11461 tcagagagtc cacatgcttg gacagttcag tgatcccctg ctccgcgaat gccatacgct
    11521 tcttgccttc aaagttgaaa ggcttagtga cgacagcaac ggtcaggata cccaaatctt
    11581 ttgccacttc agcgacgact ggtgctgcac ctgtaccggt accaccaccc atacccgcag
    11641 caataaagac catgtctgca ccttccagcg ccgcacgcaa tgcatcgcga tcctcatcag
    11701 ccgcattgcg gccaacttct ggattagcgc cagcgcccag tcctttggtg ataccgctac
    11761 cgatttgaat cgtctgtcca accgctgttt tacgcagcgc ttgtgcatcg gtatttaccg
    11821 cgaagaattc aacaccttca atgcgctcgc gcaccatgtg ttcaacagca ttaccgccgc
    11881 cgccgccgac gccgatgact ttaatcaccg cgtcattggt aagttccatt ggttcaaaca
    11941 tagtttctct ccgatttgtg cctgtcgcct gaggccgtaa tcatcgtcgg cctcataaaa
    12001 attaaaactc ttttcgcagc caactattga gtcgcttgat ccacgagcca actgatgctg
    12061 taacacgttt ttctacttca gcttcaccgt taagatgtga ctctttccca tagtgaagca
    12121 atcccaccgc cgtcgaataa tacggctcct gagcataatc cgttaaaccg gtaatgttca
    12181 gcggcgcgcc gatacgcact tgcgtatgaa acacgcgctg agcacaggct gcaagacctt
    12241 cgatctgcgc tgcgccaccg gttaatacaa tgcctgccgc caggtgatgt ttaacccctt
    12301 gttggcgaag cttttcctgc aactgcaata tctcttcgtt gaccaggttg agcagctcgg
    12361 tatagcgcgg ctcgatcacc tctgccaggg tctgacgttg cagactccgt ggcggacgac
    12421 cgcctacgct cggcacttcc acgctctcat cttttccaac gatggaaccc agcgcacaac
    12481 cgtggcgaac tttaatcgct tcggcgtcgc ttggcggcgt gccaaaggcg taagcgatat
    12541 cactggtcac gacattgcca gcataaggaa ttaccttagt gtggcgcaat gccccaccgg
    12601 tataaacggc gatatccatt gtaccacctc cgatatcgac gacgcagaca cccagttcac
    12661 gttcatcttc cgtcaatacc gaataacttg atgccagtcc ggcaaatatc agttggtcaa
    12721 ctttcagccc acaacgttca accgctttga cgatgttttt cgccatatcg ttgtgacatg
    12781 tgatcaggtg cacttttgcc tgcatccgca cgcccgaaag tcctaccgga ttcttgatcc
    12841 cttcctgata gtcaatcgca tactcttgcg ggatcacatg cagcacacga tgttcatcgc
    12901 gcacacgcac cgatttcgcg gtatggacga cgttttccac atcttcttgc gtcacttctt
    12961 cttcagaaat aggcaccata ccaatttcat tctggcagct gatgtgctta ccagaaagcg
    13021 ccagatatac cgaagagatc tgacaatctg ccatcaattc tgcctggtca atggcgcgtt
    13081 gtacgcactt gaccacggat tcgaggtcgt tcaccccgcc tttatccata ccacgtgacg
    13141 ggcagctgcc cacgccaatg atattgacca taccgtcggg cagaacttcc cctactaaag
    13201 cggcaacctt cgcggtacca atctccagtc ctactaccag ttttctgtcc gtcgccttga
    13261 tcattgttgt tctgcctgtg cctgattttg ttgctgagta gattcctctg gcggcaaggg
    13321 cgcccagcct actgccgctc cagagtcata acgcaaatca acgtagctaa tccgtttgcc
    13381 atcggtttgc gccagctgct gtaaaaccgg ataaagttct acaaagcgag ccaaacgttt
    13441 catcgtatcg ccccggccaa gattgagctt aatatcgtta ttcagcgtca actgccagga
    13501 acgccgcgcg gtcatcgccg tttccttcag agtaaatctg tcctttgcca gcatctgccc
    13561 catttcgcga tagccctgca acacttcatt ggcgctgcct tccgggccat acagcattgg
    13621 aagcacctgc ttgctggtgc gatctggcgg cacgctgaag gtgtttcctt ccgcgtctac
    13681 catatgttga tcattccacc gcgcaatcgg cacatattca accagatgaa tcttcaattc
    13741 atcaggccac tgctttctga cgctcacctg cttaatccac ggcaggcgtt gttctatttg
    13801 cgtctggatg atgttgacat cctgggtcat aaaggtaccc ggctcaccca atgccaggat
    13861 cgactgccgg atatcgtcat tacgcgtgta atggcgttca ccggtcaaca ccagctttga
    13921 gagcggcagg cgttgcgcat cttccatcca gcccaacacg acccagccgc tcaccaacac
    13981 tgtcgttaaa acggtcagca ggaaaaggat ccccgccaga cgcgttccat tattgcggcg
    14041 agaagaaacc tcttcttcgc tgtttcgcgt gttcagagca gcctgcgaca tattagtccg
    14101 ccagttccag aattcgtact accaactgcg agaagctcat acctgcctga cgtgccgcca
    14161 tcggcaccag gctgtggctg gtcatacccg gtgaggtatt ggcttccagc agataaaact
    14221 gtccatcgct gtccagcata acgtcaatac gtccccatcc tttgcaacct aacgtcgtcc
    14281 atgctttcag cactaatgcc tgcaaattgg cctcttgtga cgcatccaga cctgcggggc
    14341 agaaatactg tgtctcatca gagagatact tcgcctcata atcatagaag gttccggacg
    14401 gttgaatacg tattgacggt aaaatttctt caccgagtat cgcaaccgtg aactccggcc
    14461 cacttagcca tttttcaatc aatacttctt catcgtgctg aaatgccaat cttaatgcat
    14521 cttgtagagc attttctgct actacttttg acattcccac actggaacct tcgcggctcg
    14581 gcttaacgat aaccggcaaa cccagagcag aaatttctgc taactgctta tcgctcaggc
    14641 ctttttcaaa ctctgcgcgg gttaacgcta cccacggcgc gaccggtaaa ccggcacctt
    14701 gccacagcag tttgctgcgt agtttatcca ttgaaagcgc agatgccatc actccgctgc
    14761 cggtataagg caagcccatc agctcgagca tgccctgcag cgtaccatct tcaccgccgc
    14821 gaccgtgtag cgcgataaac actttctgaa aacccatcga cttcagttgc gtcacgtcga
    14881 cttctttcgg gtcgacagga tacgcatcaa taccaccttc acgcagcccg gctaacaccg
    14941 ctgcgccaga attcagagaa acttcccgct cagcggaagt cccgcccaac aggaccgcga
    15001 ttttatcagt catgttgttc ttcctccgga gtttgcggct tcagtttgat ttcagctaaa
    15061 gaacgggcaa tttttccgat attaccagcc ccctgaacga gaatcaggtc gttaccggtt
    15121 aataccggtg ccagcatctc ggctacctgc gcagggtccg gcaccagaat gggatcaatt
    15181 ttcccacgtc cacgaattgt gcgacacagc gaacggctgt ccgctcccgg aattggcgct
    15241 tcgccagccg gatacacttc cagcatcaac agggtatcaa cctgcgtcag cacattggcg
    15301 aaatcatcat acaagtcgcg cgtacgggta aaacggtgcg gctgaaacag cattaccagg
    15361 tttttatccg gccagcctgc gcgcgccgct ttaatggtgg cgtcaacttc cgtcgggtgg
    15421 tggccgtagt catcgaccag cattgccgta ccgcttttac cattcactgg ctccagcggg
    15481 aattcaccga ggaaatcaaa acggcgacca gtccccagga agctctccag cgcacgcaaa
    15541 atagcctcgt cgtcaattcc ctcttccgta gcaaccgcaa ccgcagctgc ggcgttcagc
    15601 gcgttatgac ggcctggcgc attcagggtg acgcgcatcg gctctttgtc ctggcgcagc
    15661 agcgtaaagt gcccctgcgg gccaatctgc tgataatctt ctacacgcac gtcggcatct
    15721 tcgctgaagc cgtaagtcgt ggtctgacgt cccacacgcg gtaacaattc gcggatcacc
    15781 ggatcatcaa cacacatcac cgcacgaccg taaaacggca ggttgtgcag aaaattaata
    15841 aaagtctgtt ttaaattctc aaagtcgccc tggtaggtat ccatgtggtc ggcttcgata
    15901 ttggtgacaa tcgccaccat cggttgcaga tgcaggaacg atgcatcact ctcatctgct
    15961 tcggcaatca ggtaccgacc atgccccaaa cgcgcatgaa cccccgccgc ttttaccagc
    16021 ccgccgttaa cgaaggttgg gtcgagcccc gcttctgcgt agatgctgga aaccatcgcg
    16081 gtggttgtcg ttttgccgtg cgttcctgca atggcgatgc catgacgaaa acgcattaac
    16141 tcagccagca tttcggcacg acggatcacc ggaatacgcg cttcatgagc tgcgacaatt
    16201 tccgggttat cggcagaaat ggcgctggaa acaacgacca cgctggcatc acgtacgttt
    16261 tccgggcgat ggttgaaata aatcgtcgct cccagattca ttaactgctg cgtgaccgga
    16321 tttggcgcta aatcggaacc actgatctga taaccttcat tggccagaac ttcggcaata
    16381 ccgcccatac cggcaccacc gatgccgaca aaatgtatgt gccgaacgcg acgcatttcg
    16441 ggcacgatgg aacgcagttt tgccaattgt tgtgtattca ttctttacgc cattaacttc
    16501 ttaaattcat acgatgcaaa aggcatcgct acaattacgc ccgggcagcc cggctcactt
    16561 catttgccac tcgctcggta gcatccggaa tggatgcagc gcgggcgcgt tctgccatgg
    16621 ttaataaggt ttctcgcgac cacccggcca gggtgttggc gacagcatcc acgctaagct
    16681 gtggctgctc gataattttg gctgcgcccg ctttttccag cggtagcgca ttccagtatt
    16741 gctggcggtc tttatgttga aacggcacaa acaacgccgg tagtcctgcc gcggcgattt
    16801 cactcaccgt taaagcaccg gagcggcaaa cgacgacatc cgcccacgca tacgccgccg
    16861 ccatatcatc aataaattcc gtcactttat gctgcggttg ccccgcttcg gcatacgcct
    16921 gttcaacgga ttgttgcgaa cctttaccgc tctgatgcca gatagtgact gaatcaccca
    16981 gtttcgcagc aacctgcggc attgtctggt taagaatgcg tgcgccctgg gaaccaccca
    17041 ctaccagcac acgaaccgga ccttcacgtc cagccaaacg ttgctgcggc aacggcagcg
    17101 ccagcacatc ggtacgcacc gggttaccca ccacttccgc attagggaaa gcgcctggaa
    17161 acgcctgcat cactttggtg gcaatcttcg ccagccattt attggttaag cccgcaatac
    17221 cgttttgttc atgaagtaca accggaatgc ctaacgacca cgcggccaga ccacctggac
    17281 ctgatacata gccgcccata ccgagtacca cgtcaggttt gtacgctttc ataatcgccc
    17341 gcgcctgacg ccaggcgttg aagatacgca gcggcgcagc tatcagtgct tttatacctt
    17401 ttccacgcag accagagata cgaatgaaat caatttcgat gccatgtttt ggcactaagt
    17461 ccgcttccat acggtcggca gtccccagcc agcgaacttg ccaaccctga gccattagat
    17521 ggtgcgcaac cgccagtccc gggaatacat gtccaccggt tccgcctgcc atcaccatta
    17581 atcgctttcc ttgaccactc atcgtgaacc tcgtacaaac gcctgcgctt tctccagacg
    17641 cgtttcataa tcaatacgca acagcatcat gatggctgtc gacataatca gtaagctcga
    17701 accaccgtaa ctgatcagcg gcaatgtcag acctttggtc ggtaacatcc ccgccgccgc
    17761 gcctacgtta accagcgcct ggaagctaaa ccagatgcca atagaacagg cgagaaaacc
    17821 ggaaaaacgg tggtcaattt ctaacgcttt gcggccaatc gacatcgcgc gaaaagcgac
    17881 gaagaatacc attaaaagtg ccagcaccac accgacatac cccagttctt cgccgataat
    17941 ggcgaaaata aagtcagtgt gcgcttccgg cagatactcc agtttttgta ccgagttacc
    18001 taaaccttgt ccccaaagtt cgccgcgacc aaacgccatc agcgattgcg ttaactgata
    18061 gccgctgcca aagggatctt cccacgggtt ccagaatgcg gtaacacggc ggatacggta
    18121 cggttcggcg agtatcagca acacaaccgc tgaaatgccc ataccgataa tggcaatgaa
    18181 ctgccacaat ttcgctcccg ccaggaacaa catcgccagc gtagtcacaa acaccaccac
    18241 caccgtacca aggtctggct gtgccagcag taacactgcc aacaccagaa tcacgcccat
    18301 cggtttcagg aagccgcgca ggttattacg tacttcgtcg cctttacgca ccagatagtt
    18361 ggcgatataa caaaacagcg acagttttgt cagctccgca ggctggatac gcagcaaacc
    18421 gagatcgatc caacgcgatg cccctttaac cgagctcccc actaccagca cgatcatcag
    18481 caggatgata gatccgagca gcatcgtggc actgtagcgt tgccagaact ccatcggcag
    18541 acgcagcgta atgatcgcca gaatgaatgc caggatcaga tagacgccat cacgcttcgc
    18601 gaagaagaac ggatcgttgg ttaagcgttg ccctatgggc attgacgccg aggtcaccat
    18661 gataaagcca atcgccgcaa ggccgaaggt cagccacagt aaggtgcgat cgtacatgat
    18721 caggctgtcg gtatcttttt cccgcgagcc catcacccag ccttttagcg ccgtggagat
    18781 ccagaccagg atactgaatc ctggcaggcg cggcattttc aggcgaggga gagataaacg
    18841 catcaaccta actccttcgc cagacgggca aactcattgc ctcgttgttc aaagttcttg
    18901 aactgatcaa ggctggcaca ggccggggag agcagaacca tatcgcccgg ctgaacacgc
    18961 ggagccagca agcgcatcgc ctgttccata gtttcggttt gttctgccac ttccgggcgt
    19021 agcgccgcca gctgcgcacc gtcacgacca aaacagtaca aacgcacgtt gtcgccattc
    19081 agatagcgcg ccagtggact gaagtccgcc gatttaccat cgccgcccag caacaaatgc
    19141 agcgtgccgt ctacctgcaa gccattcaac gccgcttccg tactgccgac gttagtggct
    19201 ttcgaatcgt taatccagcg tacgccattg tgctccagca ccacttcaaa gcgatgcggc
    19261 agaccggtga aggtggttaa cgctttcagg ctgctggcgc gcggcaaacc agctgcgtct
    19321 gccagtgcca gtgctgccag cgcattggtg tagttatgct gcccggaaag tttcatctct
    19381 ttcacgttca gcactttctc gcctttcact cgcagccagg tttctccctg ctgatgattc
    19441 aggtgatagt cccccatgtt gacgccaaag ctgacgcagc gttcatcagc accgcgaatc
    19501 ggcattgtta aggcatcatc agcattgacg acacaaactt tcgcattttc gtaaatacgc
    19561 aattttgccg cacggtattg ctgcaaacca aacggatagc ggtccatatg atcttcagtc
    19621 acgttcagaa tggtcgccgc caccgcctgt aagctggaag tggtttccag ctggaagctc
    19681 gacagttcca gcacgtacag ttcgcactca gcatcaagca gcatcaacgc tggcaggcca
    19741 atattgccac ccacaccaac gttaaccccc gccgctttcg ccatttcacc cactagcgtg
    19801 gtgaccgtgc ttttgccgtt agaaccggta atcgccacaa tcggtgcttg tgcttcgcgg
    19861 cagaatagct cgatatcgcc aacgatttcg attccggcat cagcggcagc gcttaaggat
    19921 ggatgcgcca gtgcaatacc gggactggcg acaatcagat ctgccgccat cagccattca
    19981 tcattcagac tgcccgtgtg gcgttctacg gcttcgggta atttatccag gccaggcggt
    20041 gtcatacgcg tatccataac gcgcggcgtc acaccgcgag cgaggaaaaa gtccacgcag
    20101 gaaagcccgg tgaggcccag gccgataatg acgacatttt taccctgata atcagccatg
    20161 attaacgtac cttcagcgtt gccagaccaa tcagaaccag catcagcgaa ataatccaga
    20221 aacgcacaat gacgcgcggt tccggccagc ctttcagttc atagtggtga tgaatcggtg
    20281 ccatgcggaa aatacgttgt ccgcgcagtt taaaggagcc gacctgcagg atgacagaaa
    20341 gcgtttctac cacgaacacg ccccccataa tcaccagcag gaattcctga cgtagcagta
    20401 cggcgataat gcctaacgca ccacctaacg ccagcgaacc tacatcgccc ataaagacct
    20461 gcgccggata ggtgttaaac cacaggaagc ccagtcctgc cccgactatc gcggtacaga
    20521 caataaccag ttccccggcg tgtcgcagat acggtatgtg taaatagctg gcaaagttca
    20581 tgttgccggt cgcccacgcc accagcgcaa aaccaccggc gacaaatacg gtcggcataa
    20641 ttgccaggcc gtcgagacca tcggtcaggt ttaccgcgtt gccagtaccc acaatgacga
    20701 agtaagccag cagaatgtag aacagcccca actgcggcat cacatcttta aagaatggga
    20761 ccaccagctg cgttgcgggc gtgtctttgc cggcaaggta cagggcgaag gcgacaccca
    20821 gcgcaatgac cgacatccag aaatacttcc aacgagcgat caaccctttg gtgtctttac
    20881 gcaccacttt gcgataatca tcaacaaagc caataacacc gtaacctacc agcaccacca
    20941 acacgcacca gacgtacgga ttggacgggt aagcccacag cagtacggag atcacaatcg
    21001 ccgtcaggat cataatcccg cccatggtcg gcgtaccgcg cttgctgaag tgtgattcag
    21061 gaccgtcgtt acgcaccacc tgaccaaagg aaagtttttg caaatgagca atcatacgcg
    21121 ggcccatcca caatgagatg aacagcgcgg tcagcaggct gacgatggcg cgaaacgtca
    21181 gataggaaaa gacgttaaag ccggaataat atttgaccaa atgttcggcc agccaaacta
    21241 acatgtccca ttctcctgta aagcgcgtac tacctcttcc atggcggcac tacgtgaacc
    21301 cttaactaaa atcgtaatta cctgttgctc agcaatcagt gatttaagac gcgtaattaa
    21361 cgcagtttta tcagcaaaat gttcgccaac gccgctggcg gtgctgatag catggctttg
    21421 tttacccacg cttaacacgc ggtcaatacc agcagctttt gccgcctcgc ccacctgtac
    21481 atggcaggct tcgctttcag cgcccagttc cgccatatcg cccaccacca gcacgcggta
    21541 gcccggcatt tcagccagta cctgaacagc ggcggtcatt gaaccgacat tggcgttgta
    21601 ggagtcgtcg agcagcaact ggttttctgc cagttggatg gggaacagac ggcctggaac
    21661 agctttcaga tttgccagcc ccgctttgat agcatcaagc gttgcgccca cggacatgga
    21721 gagcgcagcg gctgccagcg cattcgcaat attgtgacgc cccggcaacg gcagcagaac
    21781 atcgacgcta ccggttgggg tttgtagggt aaattccgta ccgtgcgagg tcacatggat
    21841 attggtggcg gtgaaatcgc tgttggcggc attgggtgag aaacgccaca ctttgcgtga
    21901 gccaattacg ctctgccagt tcagccagtc gttgttgtcg gcgttcataa tggcgatacc
    21961 gttttccggc aggccgctaa agatttcacc tttcgctttc gcgacacccg caagcgagcc
    22021 aaaaccttcc agatgcgccg ctgccaggtt gttgaccagc gcagcttccg ggcgagtcag
    22081 actcacagtc caggctattt cgccctgatg gttcgcgcca agttcaataa ctgcgtaatc
    22141 gtattccggc gttaagcgca acaacgtcat cggtacaccg atgtcgttgt tgagattgcc
    22201 tgccgtataa agcgtgttgc cgcactggct taaaatcgcc gccgtcatct ctttaacgga
    22261 ggttttgccg gaggaacctg tcagagcaac cacgcgcgcc ggaacttgct ggcgaaccca
    22321 tgcagccagt tcaccaaacg ccagacgcgt atccttgacg attaactgcg gcaggtcgat
    22381 atccagcgga cggctgacca gtagtgcgcc cgcgccgcca gctttcgcct ggtcggcaaa
    22441 atcgtgggca tcaaaacgtt cgcctttcag ggcaacaaac aggcagcccg gcgtcagttt
    22501 tcgggtatca gtggttacag catcaagggt gatatctgca ccttgcagtt caccgttgag
    22561 aatgtcggta agttggctaa gggttacgct aatcatgcaa tcacccccag cagacgcgcc
    22621 accgtgacgc gatcggagta gtccagacgc tgattgccaa caatctggta atcttcatgg
    22681 cctttgcccg cgaccagcac cacatcgttc tctttagcct gcataacggc gcaagtcacc
    22741 gcttcagcac ggccttccat cactttggca tgtccggcat ctaacattcc cgccagaata
    22801 tcgttgatga tggcacgcgg ttcttcggta cgcgggttat cgtccgtcac caccgccacg
    22861 tcagcaaact cttcggcaat tgcgcccatc agtggacgct tacctttatc gcgatcgcca
    22921 ccacagccaa agacacacca cagcttgccc gcacagtgca gacgcgccgc ctgtaaggct
    22981 ttttccagtg catccggcgt atgcgcgtaa tccaccacca ccgtcggttt gcctggcaca
    23041 gtgaacactt ccatacgtcc gcaaaccggt tgcagacgcg cggcggtttt caacagatca
    23101 gccaatggat agccgagtgc caacagtgtc gccagcgcga gcagcaggtt gctgacgtta
    23161 aaagcgccta tcagatggct ttcaatttcg ccatcgcccc aacttgagct aaagcgaatc
    23221 gtcacaccgc tgtcgtgata gttcacgtcg atcactttta accagcgtcc gtgacagttc
    23281 ggattaatat gatcttccat tgataccgca accgcgtccg gcagctttgc cagccaacgg
    23341 cggcccactt catcgtcggc gttaataatc gcctgaccgc aatgatgttc agaataaagc
    23401 agccatttcg cagcttcgta gtgttccata tcaccatgat aatcaaggtg atcgcggctt
    23461 aagttggtaa agaccgacgc cgcaaatttc aatgccgcca cacggtgctg taccagcccg
    23521 tgggaggaaa cttccattgc gcaaaacgtc gcgccctgct ccaccagccc cgccagctca
    23581 tgctgaacat cgactgccga accggtggta ttttctgtcg ggatcacttt ccccagcaga
    23641 ccgttaccaa cggtgcccat taccgcgctg gtttcgccaa gcagttggct ccactgcgcc
    23701 agcagctggg tagtcgtggt tttgccgttg gtgcccgtta cgcctacgag acgtaaattg
    23761 tcagagggtt catggtaaaa gcggcccgcc agtgcagata aacgctcgtt gagctggctg
    23821 agatagatga ccggtacgcc gtgcatttca cggatttcac cgtcggtcgc ctcatctttc
    23881 gcctctgcaa taatggcagc cacaccttgc gctatcgcct gcgggatata tcgacgcccg
    23941 tccgcctgat gacctactac agctacaaag agatcgcccg ccgcagccac acggctgtcg
    24001 agtgtcatct ctcgcagtgc tcgcgaaggt gcgtctggca cccacggagc aagaaggtcg
    24061 cgcaaattac gatctgccac ctgtcccctc gccttgatta atcacaaatt catttttatc
    24121 gcccgttgtc agcgcatccg gctcgatgtt catggtacgc aatacgccgc ccatgatggc
    24181 accaaagacc ggcgcggaaa cggcgccgcc gtagtattta cccgcctgcg gatcgttgat
    24241 aacaacaacc agcgcgaagc gcggctgact cgcaggcgca acgcctgcgg tataagcaat
    24301 atatttattg atgtagcgac cgtccggccc gacctttttc gcggtaccgg ttttaatggc
    24361 gatacgatag cctttaatcg ccgccttcac gccgccgccg cccggtagcg ccacgctttc
    24421 catcatatgc accacggtgc ggacaatgga ttccgggaag acacgttcac cgggaaccgg
    24481 ggggtcaact ttggtaatcg acagtgggcg ataaatgccg tagctgccga tagttgcgta
    24541 gactcgcgct aactgtaacg gtgttaccat tagcccgtag ccgaaagaga aggtggccct
    24601 ctctatgtca gaccaccgtt gtttttgagg atataagcca ctgcgttctc cgaccaaccc
    24661 caaattggtc gcttttccca gtccaaaacg tgagtaagta tctactaacg ctgaggacgg
    24721 catcgctaac gccagcttgg aaacaccgac gttactcgac ttctgtaata ccccggtcag
    24781 ggttaattcg ctgtagcgtg ccacgtcttt gatttcgtgg ccgttaattc gataaggaat
    24841 ggtattgagt accgagtttt cccgcaccac gccacgttgc aacgcggtca tcaccaccat
    24901 cggtttaacc gttgagcccg gttcaaacac gtcggtgatg gtacggttac gcatcgcctc
    24961 tttcggcgtg ccgctcagat tgttagggtt gtatgacggg ctgttagcca tcgccagcac
    25021 ttcacctgtg ttgacatcca ccagcacggc gctaccagat tcagccttgt taaaggccac
    25081 cgcgttgttc agttcgcgat aaaccagcgc ctgcaggcgt tcatcaatac tcagcgccag
    25141 gttgtgcgct gcctggctgt cagtagaaga aatatcttca attacgcgac catagcggtc
    25201 tttacgcaca atgcgctcac ccggctgccc ggtaagccat ttatcgaaac tcttctcaac
    25261 gccctcaatc ccttgactat cgacgttagt aaagccaatg aggtgagcag tcacttcgcc
    25321 ggatggataa taacggcgag attcttcacg cagatgaata cccggcagtt tcagtttttt
    25381 gatgtagtcc gccatgtcag ggtttacctg acgcgccaga taaataaagc gccctttcgg
    25441 gttggcgtta atgcgggcag aaagctgatc cagcggaata ttgagcgcgt tagccagcgc
    25501 cttccagcgg tcaccgacgc tgataccgcc agcgtcatgc acttctttcg ggtcagccca
    25561 aatcgctttt accggcacgc tcaccgctaa cgggcgacca gaacggtcag taatcatgcc
    25621 gcgggaggtg gaaacttgct gaacgcgaag agaacgcatg tcgccctctt tcaccagcat
    25681 atccggggag ataacttgta accacgctac gcgtccgagc agaaaagcca gcgccaggag
    25741 aatacagccg cataacaacg caaaacgcca actaataaag ttggcatgtt cttcctgacg
    25801 ttttggtttc tgcgttttcg ccgctgcttt catgcgtcga gtttatcctt atttttgcac
    25861 tacgatattt tcttgtgacg gatcaacatg ctgcatttgc agcttttccg tggcgatcct
    25921 ttccacccgg ctatggtcgc cgagcgcatt ctcttcaagg atcaggttgc gccattcaat
    25981 gtctaaagca tctcgctcca gcaccagttg ttcgcgctga gcggtcagta aacgggtatg
    26041 gtgcgccgtg gttaccacgg tcaccgccgt caaaataatg caaatgaaca ggcagagtgg
    26101 cagcttccca aatcgcaaaa gatcgtcacc gataacacca ggcaatgcat ggcgctcgtg
    26161 gcttcccatc gatcctttaa ctttgcttag agcttctgtc actctgctga tcatgcgttc
    26221 gtcctctctg cgatacgcag aactgaacta cgggcacgag gattctcagc cacctcttct
    26281 tcgcccggca ttaacttgcc taatgctcgc agctgacggc cacccagttt tttgagctgc
    26341 tcttcagtca tcggtaaccc tgccggaact tgcggaccgc ggctgttttc acgcataaaa
    26401 cgtttcacaa tacggtcttc cagcgagtgg aagctgatga tcgaaagccg cccacccggg
    26461 gccagcacgt tgagcgagct ttttagcgcc tgctctatct cctccagttc actgtttacc
    26521 caaatgcgca ccgcctggaa ggtacgggtc gcgggatgtt taaacttatc tttcaccggc
    26581 gttgcagcag ccacgacttc cgccagttct ttggtgcggg tcatcggctg ttcgcggtta
    26641 cgctcgacaa tggcgcgggc aatgcgtttg gcaaaacgct cttcaccata ggttttcaat
    26701 acccaggcga tatcggcttc ttctgcggtt tgtagccatt cagcggctga ctgcccacgg
    26761 gttgggtcca tacgcatgtc cagcggacca tcgcgcataa aggaaaagcc acgttcagca
    26821 tcatcaagtt gcggtgaaga gacgccaaga tcgaggagaa tgccgtcgat cttgccgata
    26881 agatcgcgct cggcaacgta ttcgcccagc gcggagaaag gtccgtggat gatggagaag
    26941 cgcggatcat caatagtctt cgcaacggcg atagcctgcg ggtcgcgatc gatcgccagc
    27001 aaacgcccct cttcgccaag ctgcgagagg atcagacgtg agtgaccacc gcgaccaaaa
    27061 gtcccatcaa tgtagatgcc atcaggacgg atattgaggc cattaacggc ttcatccagc
    27121 agcaccgtag tatgtttata gttttccatc attttataga gacaagtcct gcagtcgctc
    27181 cgataagtct ccagtagcca actgctctgc gtcgatatct tccttgacct gttgatgcca
    27241 agttgtttca tcccacagct caaacttgtt gaactgtcca accagcatca cttcttttgt
    27301 cagcccggca tgttgccgca gtactggcgc gattaacaat cgacctgcgc catccatctg
    27361 acattcgctg gcatgaccta acagtaggcg ctgcacacgg cgctcaactg ggttcatgct
    27421 cgacagacgc gataattttt gctcgataat ttcccattca ggcagggggt aaagcagcag
    27481 gcacgggtga tgaatgtcaa tggtgcaaac catttgaccg gcagcgttct caagcagctg
    27541 ttcccgataa cgggtaggca ctgataagcg ccctttgctg tcgagattga ctaacgttgc
    27601 tccccggaac atgccagcct caccccttat tcccactttg ccccacaatt tcccactgaa
    27661 aggagtttac ggagcggagg aaaagcttgt caagccagtc acaaggcata ccgaggtaaa
    27721 aaacccgatg tttacggcta atatcagcgt tgtttatttt acgtccaacg cgcgaagcaa
    27781 attaacgtta tgaatagctg taagaaaaaa tccctcatcg tcatcactgt tttaaaatga
    27841 ctcactattt tccacagcaa atataaagtg tcagtttgcg acgcgagcgg cattttagga
    27901 catatcttcc ccggttaaca gtccttgtta cgtctgtgtg gcgcttgatg ccagcgggtt
    27961 tgcgcaaaag caaagaaaag tgtttgttaa atcggcaatt catcgccgca tgtaacaaaa
    28021 taatgcaaaa acgcgctgtc gatatcactt aattattgtg aatttcagct ggtgagttaa
    28081 ttaacgggcg ggtttaaaac catttatcaa taatagtcag gaatagtctt atttacttta
    28141 agcatattga tgtccagtcc cgtactctac gcgccagagg gaaattcacc tggcgcgtat
    28201 ttttgttcgc ggcttagcta cggctgagca caccgcggcg atagagatta cgtttaatgc
    28261 gcgttaaacc aggttttggc ttacgcggtt cgtccaggct tgccaggaca atctccagca
    28321 cacgctctgc gacatcgcgg tgacgttgag ccactgccag caccgggcac tgtaagaagt
    28381 cgagcagttc gttatcgcca aaggtggcaa ttgccaggtc agaaggcagt ttaccatcgc
    28441 gacgtagcgt gacatccatc accccttgca acaacgcaaa cgacgttgtg aacagcgcct
    28501 gcggcatcag atgcgtttcc agccattttt cgaataactg ggcagccgcc tcccgctcat
    28561 agctgttggc atacaggaaa tgcacttcgc gcggatcatc tttccaggca gtacggaaac
    28621 cttgttcacg caggaagctg acagaaagct ccggcagcgc gccaagataa agcaccgtct
    28681 cggcgggaaa cttacgtaac tcttccgcca gcatttcggc atcatcctga tcggcaccaa
    28741 ccacgctggt gaaatgttca cgatcgaggg cgcggtccag cgcgacaatc gggaacgggg
    28801 cgttagccca gcgttgataa aaaggatgct caggtggcaa cgacgtcgaa acaataatgg
    28861 catcaacctg acgctgtaaa aggtgctcaa tgcagcgcat ttcgttgtct ggctgatctt
    28921 ctgagcaggc aatcagcagt tgataacccc gttgccgcgc ctggcgctca agatagttag
    28981 cgatgcgggt atagctggtg ttctccagat cggggatcac aagaccaata gaacgtgtgc
    29041 gtccagcacg aagcccagct gccacggcgt tcgggtggta attgtgctca cgcaccacag
    29101 ccatgacttt ttcaacggtt ttgtcgctca cacggtattg cttcgctttg ccgttaataa
    29161 catagcttgc agtggtccgc gacactcccg ccagccgagc gatttcatcc agtttcacaa
    29221 ttgccccttg cgtaaaatgt aaaaaccata gccattgtac tggtatgggt taaattcatt
    29281 aacatctaag cgcagaaaat taactgcggc aactgctttt attccaggtt cccactgatt
    29341 tcgcaaaaaa agcccagcgt gacatgttgg gctgcaaatt gcacactgag atcattctca
    29401 acgcattatt ttatcgccgc gcgaaagtcc gaccacacca gagcgagcaa cttccacaat
    29461 tttcgccacc tcgcgaatcg atgctaaaaa tgcatcaagc ttatcgctgg tgcctgctaa
    29521 ttgaacggta taaagcgagg gggtgacatc gataatttgc ccacggaata tttccgtatt
    29581 acgtttcact tcgtcacgcc cgtaaccgct ggcctgaatt ttcaccagca taatttcccg
    29641 ctcaacatgc gcgccctgcc ccaactcact cacgcgcaag acatcgacca gcttgtgtaa
    29701 ttgcttttcg atctgctcaa gtactttttc attgcccacg gtctggatgg tcatacgcga
    29761 taatgtcgga tcgtcggttg gcgcaacggt caggctttca atgttgtagc ctcgctggga
    29821 aaaaaggcca atcacgcggg ataacgcgcc tgattcattt tcgagtaaga ctgataatat
    29881 ccggcgcata atcaggttct ctccgttttg cttaaccaca tttcatccat tccgcccccg
    29941 cgaatctgca tcgggtagac gtgctcactg ccatcgacgg taacatcaac aaacaccagg
    30001 cgattattgc gcacctgttc cagcgcctcg ctaagtttgc tttccagctc atgcggatga
    30061 gagatctgga tcccgacgtg accataggct tccgccagac ggacgaaatc gggtagtgat
    30121 tgcatataag attgtgaatg acggcctgaa tagatcatgt cctgccactg tttcaccatc
    30181 cccagatagc ggttattgag attcaccacc agtacgggca actcgtattg caacgcggta
    30241 gacagttcct gaatgttcat ctgaatactg ccgtcgccag tgacgcaaac cacggtttct
    30301 tctggcaacg ccattttgac gcccagtgcc gcaggtaaac caaaacccat cgtgccgagg
    30361 ccaccggaat tgatccagcg acgcggtttg tcgaatggat aataaagtgc agcaaacatc
    30421 tggtgctgcc cgacatcgga cgtcacgtaa gcgtctccct tcgtcaaccg ccaaagagtc
    30481 tcgatcaccg cctgcggttt aatcttttca ctgtgagtgt catatttcag gcactgacga
    30541 gcgcgccact gttcaatttg ctgccaccag tcgcggatct catccagtgg ttgatgggcg
    30601 gattcttgcg acaagagttc aagcatttgt tcgaggacct ggcgagcatc ccccacaatc
    30661 gggatatccg cagtcacggt tttagaaatg gaagtaggat caatatcgat atgcagaaca
    30721 gtggcatttg ggcagtactt tgccagattg ttcgtcgttc ggtcatcaaa tcgtaccccg
    30781 acggcgaaaa tcacatccgc gttatgcatc gtcatattgg cttcgtaggt accgtgcatt
    30841 cccagcatgc ccagtgcctg acgatgcgtt gccggaaacg cccccagccc catcaatgag
    30901 gaaacaacgg gcagattcaa cgcctccacc gtttctttca actgctgatg gcagcccgcc
    30961 atgattgccc caccgcctac gtagacaacc ggtttttttg ccactaccag cgtttgcaga
    31021 gcacgcttaa tttgcccttt atgtccggta gtagtgggat tgtaagaacg catactgacc
    31081 gactccggcc agacataggg taatttgttc gccggattaa gaatatcttt cggtaaatca
    31141 acgactactg gtccaggacg accacttgcc gccagccaga aagccttttt cagcacctgc
    31201 ggaatgtctt ctgtttgctt aaccagaaaa ctgtgtttaa ccactggtcg cgaaatcccc
    31261 accatgtcgc actcctgaaa ggcatcgtaa cctatcaacg aggtcgctac ctgcccggaa
    31321 aggacaacta atggaatgga atccatataa gcggtggcga tgccggtaat cgcattggtc
    31381 gcccctggac ccgacgttac cagcacgacg ccgacttccc cggtcgcgcg cgccagacca
    31441 tcggccatat gcactgccgc ctgctcatga cgaactaaaa catgatcgat accacccacg
    31501 gtatgcaatg catcgtaaat atcaaggact gcgcctccag gataaccgaa tacttgttta
    31561 acgccctgat cgataagcga tcggacgacc atctcggctc cagacaacat ctccatggcc
    31621 tgcctcactg tttgatggaa aaaatgtgta aaacacatta accgtccgat cttgcttaag
    31681 caattggcag ccagagacat aaacgcaggg taataagaat aaacaggagg aaagatgaaa
    31741 agaaacgata aaatcctcca ttacgaagga ttaaattgat aataaatcgg ctgaatccca
    31801 caacttacag caatttctct atttttttaa ttaatcctaa aatagagagc ccacacattc
    31861 agaataaaat tctacattca ataaaaaatg cagaataaac cagacattca tgtctgacct
    31921 attctgcatt cagttagcgt ttgcaaattg agactaattg ttcttccatc cactgatggc
    31981 ctttatcgcg cccggcagct tcatgccatg agagataaca ggttctgctg ttttgtttta
    32041 acggcagcgg taatacctgt aattctaagg attcagcgaa ctcttcagcc agccaacgcg
    32101 gcgcaatagc gaccaaatgc gtttgcgaca ccacgctaag tacgctcatc attgccatgc
    32161 cctgatacgc gatactggct tgcttatcta ccgtgtcata ccaaggttga ctaaatgacg
    32221 cgaaacgatc gagcgaaacc gccgcatgtt gttcgttata aacatcatgt ttcagtaacg
    32281 ggcctttaat tgttggatga tttttgctgg ctaccagcac catttcatct ttaaataatg
    32341 gcacgctggt aaattcagga cgatggaagt cttcataact aatcacaaac tccgtttcct
    32401 gataacgcag ctgatgttca gtgttctgat ttaatgaaga cttgaacata acatgtatat
    32461 ttggcgcaat ctgctcaatg tgattataaa tctgcgaggt cagaatgctg tctaacgggc
    32521 tgcaaacaca aagatgaaat acacgttcac tactcgcggg ttcaaaacct gaaccaggca
    32581 attcattttg tactagttgc aatgcctggc gaactgaacc aaaaagttga aatgcgcgag
    32641 cagtcggttg aataccacgg ccataacgaa caaaaagctc gtcattaaac atcaccttca
    32701 ggcgtgcaac agcgttactg accgcaggtt gcgacatacc cagaacatga gcggcacggg
    32761 taatgttttg ctcctgcatc acggcatcga aaacggttaa taagttgaga tcgaccatgc
    32821 gtagctgtgg cttgcttaac tccgccgtct ctgaatgatc tgtttgtacc tctggcatac
    32881 ttaactccac tgtcacactt aactcccttt cccttattgg aatgcagaga aataatcctg
    32941 aagaatatga tttatc
//
LOCUS       DADLFA010000025        32826 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000025 DADLFA010000000
VERSION     DADLFA010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32826
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.025"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..729)
                     /gene="emrY"
                     /locus_tag="JQH65_001006"
     CDS             complement(<1..729)
                     /gene="emrY"
                     /locus_tag="JQH65_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HAY6138493.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTV"
     gene            complement(729..1892)
                     /gene="emrK"
                     /locus_tag="JQH65_001007"
     CDS             complement(729..1892)
                     /gene="emrK"
                     /locus_tag="JQH65_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HAY6138494.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYVYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLT
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            2308..2922
                     /gene="evgA"
                     /locus_tag="JQH65_001008"
     CDS             2308..2922
                     /gene="evgA"
                     /locus_tag="JQH65_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HAY6138495.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            2927..6520
                     /gene="evgS"
                     /locus_tag="JQH65_001009"
     CDS             2927..6520
                     /gene="evgS"
                     /locus_tag="JQH65_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HAY6138496.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDIIPGAIYSEDRENNVSFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILITFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(6576..7721)
                     /gene="yfdE"
                     /locus_tag="JQH65_001010"
     CDS             complement(6576..7721)
                     /gene="yfdE"
                     /locus_tag="JQH65_001010"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HAY6138497.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDYDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(7795..8739)
                     /gene="yfdV"
                     /locus_tag="JQH65_001011"
     CDS             complement(7795..8739)
                     /gene="yfdV"
                     /locus_tag="JQH65_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HAY6138498.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIVYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(8809..10503)
                     /gene="oxc"
                     /locus_tag="JQH65_001012"
     CDS             complement(8809..10503)
                     /gene="oxc"
                     /locus_tag="JQH65_001012"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HAY6138499.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNHPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVIGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSSVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(10557..11807)
                     /gene="frc"
                     /locus_tag="JQH65_001013"
     CDS             complement(10557..11807)
                     /gene="frc"
                     /locus_tag="JQH65_001013"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HAY6138500.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(12320..12952)
                     /locus_tag="JQH65_001014"
     CDS             complement(12320..12952)
                     /locus_tag="JQH65_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HAY6138501.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            13248..13523
                     /locus_tag="JQH65_001015"
     CDS             13248..13523
                     /locus_tag="JQH65_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6138502.1"
                     /translation="MKVNLILFSLFLLVYIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(13600..13842)
                     /locus_tag="JQH65_001016"
     CDS             complement(13600..13842)
                     /locus_tag="JQH65_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HAY6138503.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTEIFMFLAPACGLMIRFMVGYGRR"
     gene            14195..15115
                     /gene="lpxP"
                     /locus_tag="JQH65_001017"
     CDS             14195..15115
                     /gene="lpxP"
                     /locus_tag="JQH65_001017"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HAY6138504.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHCRFKTRPVGESSLYI"
     gene            15471..15542
                     /gene="ypdK"
                     /locus_tag="JQH65_001018"
     CDS             15471..15542
                     /gene="ypdK"
                     /locus_tag="JQH65_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HAY6138505.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(15607..16845)
                     /gene="alaC"
                     /locus_tag="JQH65_001019"
     CDS             complement(15607..16845)
                     /gene="alaC"
                     /locus_tag="JQH65_001019"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HAY6138506.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRQDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWIVEMPKASMYVWAKI
                     PEPYAAMGSLVFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            17222..18919
                     /locus_tag="JQH65_001020"
     CDS             17222..18919
                     /locus_tag="JQH65_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAY6138507.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            18934..19668
                     /locus_tag="JQH65_001021"
     CDS             18934..19668
                     /locus_tag="JQH65_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY6138508.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            19681..20538
                     /locus_tag="JQH65_001022"
     CDS             19681..20538
                     /locus_tag="JQH65_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY6138509.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNSQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYTQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(20541..23036)
                     /gene="ptsP"
                     /locus_tag="JQH65_001023"
     CDS             complement(20541..23036)
                     /gene="ptsP"
                     /locus_tag="JQH65_001023"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAY6138510.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQAQRVLEEYIQVRFIDSDSIQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASSVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRESDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLDYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPIDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(23061..24098)
                     /gene="ypdE"
                     /locus_tag="JQH65_001024"
     CDS             complement(23061..24098)
                     /gene="ypdE"
                     /locus_tag="JQH65_001024"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAY6138511.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGTRSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHSAELPAEVWLVASSSEEVGVRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQICNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMQQLLSALIKRLT
                     RETVVQLTDFR"
     gene            complement(24098..25183)
                     /gene="ypdF"
                     /locus_tag="JQH65_001025"
     CDS             complement(24098..25183)
                     /gene="ypdF"
                     /locus_tag="JQH65_001025"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAY6138512.1"
                     /translation="MTLLASLRDWLKEQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSSYFVEVEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWKSELNAKLVSATPDVLRQIKTPEEVEIIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGVLPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFDVYQIVLQAQLAAISAIRPGVRCQQIDDAARRV
                     ITEAGFGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(25198..26445)
                     /locus_tag="JQH65_001026"
     CDS             complement(25198..26445)
                     /locus_tag="JQH65_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAY6138513.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGVIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(26467..26793)
                     /locus_tag="JQH65_001027"
     CDS             complement(26467..26793)
                     /locus_tag="JQH65_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HAY6138514.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(27012..27977)
                     /gene="glk"
                     /locus_tag="JQH65_001028"
     CDS             complement(27012..27977)
                     /gene="glk"
                     /locus_tag="JQH65_001028"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HAY6138515.1"
                     /translation="MIKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            28181..29437
                     /locus_tag="JQH65_001029"
     CDS             28181..29437
                     /locus_tag="JQH65_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HAY6138516.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTINIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            29552..29878
                     /locus_tag="JQH65_001030"
     CDS             29552..29878
                     /locus_tag="JQH65_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HAY6138517.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(30019..31257)
                     /gene="mntH"
                     /locus_tag="JQH65_001031"
     CDS             complement(30019..31257)
                     /gene="mntH"
                     /locus_tag="JQH65_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mn(2+) uptake NRAMP transporter MntH"
                     /protein_id="HAY6138518.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            31593..32795
                     /gene="nupC"
                     /locus_tag="JQH65_001032"
     CDS             31593..32795
                     /gene="nupC"
                     /locus_tag="JQH65_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HAY6138519.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
BASE COUNT         8633 a         8180 c         7547 g         8466 t
ORIGIN      
        1 tactgttaat attattattg tactcgagtt gaaccaatcc agatcgcgcc ctttatcaag
       61 cataatttgc aagccaccaa caccgagcac taacagggtc agtcctggta gattcatttt
      121 gaccggtgaa gtctcagttt ctcttccttt aagtaaggtt aagcataatg tcaggacgat
      181 aatccccata gggacattga ttaaaaatat ccaaccccag ctaaagttat cacaaatata
      241 accgcccaat atcggcccac atatcggagc gataatcacg gtcattgacc ataatgccag
      301 agcaaatgtt cttttttctg gcggataatt ccttaataat aaactctgtg acagtggaat
      361 taacggcccc gccattaacc cctgaacgac tctaaaaaat atcagcacat caagattggt
      421 cgataggcta cacattaatg aagacagcga aaaaaaggtt actgaaagta aaaataatct
      481 taattcgcct attctttgtg ccaacctgcc agtaacaggg atcgcaatgg catttgctac
      541 accaaacgag gtgataaccc aggtgccttc gtctgttgat gctcccagaa agccagatat
      601 tgtcggtatt gcgacgttag aaatagtgga atccaacatt tgcataaatg tcgctaatga
      661 caatgcaata gtgacgcacc ataacgtccc accggttaat ggtgccggag ttgatttagt
      721 gattgccatt aaagttgtcc attatgcgaa ataatgttgc taatttcttt ttctatcgga
      781 ctggtatcga taactaaagc cttactggta taagccggca tggaggtcac ggttgaagcc
      841 agctcaggca tctcggcaat gtcttcgttc ttcgtatcaa tagttgctgt catcgataaa
      901 ccaatacgca agggatgttc catgagttct tttggatcaa gagaaacttc aaccggtaca
      961 cgctgaacga ttttgatcca gttccctgtc gcattttgtg caggtaataa ggagaacgca
     1021 ttgccggttc ccatattgat ccctgtcacc cgaccatgaa acacaacatt ttcaccataa
     1081 agatcgctga taatattgac cgattgacca atccgcacat ccgtgagttg tgtttcttta
     1141 aagttggcat taacccacat ttgacgtgcc ggtacgacag ccattaacga ttgtccgggg
     1201 ctcactgttt cgccgacctg aacacttctc tgggcaatat agccggtaac cggactctta
     1261 atatccgtac gtttaagcgc caaccaggct tctttagttg catccgccgc ttcaacgact
     1321 tgtggctgac ggtttaatgg tgtgttcatt actaaagctt tattcgcttt ataagcctgg
     1381 atagcggcat tcaatgccgc tttgctactt attaacgtat ctttggtatg ctccagcgtt
     1441 tcttttgaaa taaccccctg cttcgctaac ggcactcgac ggttataatc ttctaaagat
     1501 tgttgatact gaatacgtgc tgaagcgact tcggcactgt attgtttatc ctgtaagtat
     1561 agtttattcg tttgccgaac aatatttgtc agattatttt tagctttatt gagtgcgata
     1621 gtggcatcag ttttatccag tgaaactaaa atgtcacctt gtcgaacgta gttcgtatct
     1681 ttatgattaa cgacagtgac actacctgag acttgtgcag aaattggatc tgcattcccc
     1741 gtgacatagg cgtcatctgt actaatcatg tcttctaatt ccattgacca atagacatag
     1801 gcacctgaaa acgcaataaa taaaactacc gccaataaag aaaagtattt tcttctgtta
     1861 gaatgttttt tatttgaatt aatctgttcc actattatct ctcatttctc atagatgaaa
     1921 tttatgaatt gaatgaaaag agcattcatt cttcatatca aacacagtgc atgtgttgca
     1981 cattatccca ggaacgttgg aggcctaatt taaatgaggt gcaaaaaaaa caacagagga
     2041 ctaaaccgtg gcttttgcaa tacaattctt acgcctgtag gattagtaag aagacttata
     2101 gtgctaactt gaaactataa atcatcggta caacccctga ttttattgtt gacatttcat
     2161 ttatgccgac tatttatatg gtatacttgt cgaattatct taaaggaagc tcagattttc
     2221 ttatttttat tgagaaaatg agatgacgcc ttatgtctgt attactacag ggagaaggga
     2281 gatgcttcat tgcaaaggga ataatctatg aacgcaataa ttattgatga ccatcctctt
     2341 gctatcgcag caattcgtaa tttattgatc aaaaacgata ttgaaatctt agcggagttg
     2401 actgaagggg gaagcgccgt tcagcgggtg gaaacactta agcctgatat cgtcatcatt
     2461 gatgtcgata tccccggagt taacggtatc caggtgttag aaacgctgag gaagcgccaa
     2521 tatagcggaa ttattattat tgtctccgct aaaaatgacc atttttacgg gaaacattgt
     2581 gctgatgctg gcgctaatgg attcgtgagt aaaaaagaag gcatgaacaa tatcattgcg
     2641 gctattgaag ctgcaaaaaa tggctactgc tatttcccct tctctctcaa ccggtttgtt
     2701 ggaagtttaa cgtccgacca gcaaaaactc gactccttat cgaaacaaga aattagtgtc
     2761 atgcggtata ttcttgatgg caaggataat aatgacattg ctgaaaaaat gttcatcagc
     2821 aacaaaactg tcagcactta taaaagtcgc ctgatggaaa aattagaatg taaatcactg
     2881 atggatcttt acacattcgc gcaacgtaac aaaatcggct aatcacatga agtttttacc
     2941 ctattttttt cttctctgtt gtggtctttg gtcgaccata agtttcgcag acgaagatta
     3001 catcgaatat cgtggcatca gtagtaacaa ccgtgtcaca cttgatccag tacgtctgag
     3061 caacaaggaa ttacgttggt tagcgagcaa aaaaaatctt gtgattgcag tacacaagtc
     3121 ccaaacggct accttgttgc ataccgattc gcagcaacgg gttcgtggta ttaatgctga
     3181 ttatttaaat cttttaaaaa gagcgttaaa tatcaaatta acactccggg aatacgccga
     3241 tcatcaaaaa gcaatggacg cgctggaaga aggtgaagtc gatatagtgt tatcacattt
     3301 agttgcttcg ccgcctctta atgatgacat tgctgcaacc aaaccactga taattacctt
     3361 tccggcgctc gtcaccaccc ttcacgattc aatgcgaccg cttacctcat caaaaccagt
     3421 aaatattgct cgagtagcaa attatccccc tgacgaggta attcatcaat catttccaaa
     3481 agcaacaatt atctctttta caaatttata tcaggcatta gcatccgtct cagccggaca
     3541 gaatgattac tttattggta gtaacatcat taccagcagt atgatttccc gctatttcac
     3601 tcactcctta aatgtagtga aatattataa ctcaccgcgt caatataatt ttttcttgac
     3661 cagaaaagaa tctgtcgttc ttaatgaagt actcaatcga tttgttgatg ctttaacaaa
     3721 tgaagttcgc tatgaagttt cacaaaattg gcttgataca ggaaacctgg cctttctgaa
     3781 caaaccatta gaactcactg aacatgaaaa acagtggatt aagcaacatc ccgatttaaa
     3841 ggtgctggaa aatccttact ctcctcccta ttctatgacg gatgaaaatg gctcggttcg
     3901 gggcgttatg ggggatattc ttaatattat taccttgcaa acaggtttaa atttttctcc
     3961 gatcaccgtt tcacacaata tccatgccgg aacacagctt aaccccggtg gatgggatat
     4021 aatacctggc gctatttata gtgaagatcg agaaaataat gtttcatttg cagaagcttt
     4081 cataacaacg ccatacgttt ttgtcatgca aaaagcacct gacagtgaac aaacattaaa
     4141 aaaaggaatg aaagttgcca ttccatatta ttatgagctg cattcgcaat taaaagagat
     4201 gtatccggag gttgagtgga taaaagtcga taacgccagc gctgcatttc acaaggtcaa
     4261 ggaaggtgaa cttgatgctc tggtcgcgac acagctaaat tcgcgttaca tgatcgatca
     4321 ttactatcct aatgaactgt atcattttct tattcccggc gttccgaatg catcgctttc
     4381 gttcgctttt cctcgcggag aaccggaact taaggatatt attaataaag cactgaatgc
     4441 aattccccca agcgaagttc tgcgcctgac ggaaaaatgg attaaaatgc ccaatgtgac
     4501 cattgacaca tgggacctct atagcgagca attttatatt gttacgacat tatccgtttt
     4561 attagttggc agtagccttt tatggggatt ctacctgtta cgctcagttc gtcgtcgtaa
     4621 agtcattcag ggtgatttag aaaaccaaat atcattccga aaagcactct cggattcctt
     4681 accaaatcca acttatgttg taaactggca aggtaatgtc attagtcata atagtgcttt
     4741 tgaacattat ttcactgcgg attactacaa aaatgcaatg ttaccattag aaaacagtga
     4801 ctcacccttt aaagatgttt tttctaatgc gcatgaagtc acagcagaaa cgaaagaaaa
     4861 tcgaacaata tacacacagg tatttgaaat tgataatggc atcgagaaaa gatgcattaa
     4921 tcactggcat acattatgca atctgccagc cagcgatcat gctgtttata tttgtggttg
     4981 gcaagatatt accgagacgc gtgatttaat tcatgcactc gaagtagaga gaaataaagc
     5041 gatcaatgca actgtcgcaa aaagtcagtt tctggcaaca atgagtcacg aaataagaac
     5101 accaataagc tccattatgg gcttcctgga acttctgtcg ggttctggtc ttagcaagga
     5161 gcaacgggtg gaggcgattt cacttgccta cgccaccgga caatcactcc tcggcttaat
     5221 tggtgaaatc cttgatgtcg acaaaattga atcgggtaac tatcaacttc aaccacaatg
     5281 ggtcgatatc cctactttag tccagaacac ttgtcactct ttcggtgcga ttgctgcaag
     5341 caaatcgatc gcattaagtt gcagcagtac atttcctgat cattacctgg ttaagatcga
     5401 ccctcaggcg tttaagcagg tcttatcaaa tttactgagc aatgctctca aatttaccac
     5461 cgagggggca gtaaaaatta cgacctccct ggttcacatt gatgacaacc acgctgttat
     5521 caaaatgacg attatggatt ctggaagtgg attatcgcag gaagaacaac aacaactctt
     5581 taaacgctac agccaaacaa gtgcaggtcg ccagcaaaca ggatctggct taggcttaat
     5641 gatctgcaaa gaattaatta aaaatatgca gggcgatttg tcattagaaa gtcatccagg
     5701 cataggaaca acatttacga tcacaatccc ggtagaaatt agccagcaag tggcgactgt
     5761 cgaggcaaaa gcagaacacc ccatcacact acctgaaaag ttgagcatat taatcgcgga
     5821 tgatcatccg accaacaggc tattactcaa acgccagcta aatctattag gatatgatgt
     5881 tgatgaagcc actgatggtg tgcaagcgct acacaaagtc agtatgcaac attatgatct
     5941 gcttattact gacgttaata tgccgaatat ggatggtttt gagttgactc gcaaactccg
     6001 tgagcaaaat tcttccttac ccatctgggg gcttacagcc aacgcacagg ctaacgaacg
     6061 tgaaaaaggg ttaagttgcg gcatgaactt atgtttgttc aaaccgttga ccctggatgt
     6121 actgaaaaca catttaagtc agttacacca ggttgcgcat attgcacctc agtatcgcca
     6181 ccttgatatc gaagccctga aaaataatac ggcgaacgat ctacaactga tgcaggagat
     6241 tctcatcact ttccagcatg aaacgcataa agatctaccc gctgcgtttc aagcactaga
     6301 agctggcgat aacagaactt tccatcagtg tattcatcgc atccacggtg cggctaacat
     6361 cctgaatttg caaaagttga ttaatattag ccatcagtta gaaataacac ctgtttcaga
     6421 tgacagtaag cctgaaattc ttcagttgct gaactctgta aaagaacaca ttgcagagct
     6481 ggaccaggag attgctgttt tctgtcagaa aaatgactaa atagcggctc ccacaatgtt
     6541 caaatgtggg agctatttac cagcacatct tacatttatg atgagaactc ctggcgaatt
     6601 tgttccccat gctggtcgag cgttgccgct cccggcataa catgcgggtc cgcgcagccg
     6661 ctgattttta tcggattacc cggcatcatt attcccccgg cttcaatcaa catatttctc
     6721 gcctgagttt gtggcaattt aatggcctca gccacactta ataacggcgc gacgggtaca
     6781 ccaacttcat gtattctggc taaccaaact tcagctgcct gcgtttttaa cgtccgctca
     6841 atatattgtt taagaatagc ctggttttgt acgcgtaaaa tattgctgct aaatcgggga
     6901 tcattaacca gttccgtaag ctccagtgcc tggcataacg cagaaaaaag cttgtcatta
     6961 ccacaacaaa tcgtaatcgg cttatcctga gtattgaaaa catcaaaagg tgccatgtag
     7021 ggatggcgat ttcccagacg ttgtggtgac ttccctgtcg cgatatatgc catcagacca
     7081 tgctccagaa aactcagcgt ggcatcaaac atcgctatat cgacatgcgc ccctctctgg
     7141 ctcttttcgc ggccataaag tgcactcact attccgctga ataaatagac accgccgcat
     7201 agatccgcaa gagatgtacc aacgcgcact ggcggagcat caggatatcc tgtttccatc
     7261 attatcccgc tcattgcctg aatgatggta tcgtaggcag gagcatcttt tagcggaccg
     7321 gtatgtccga aacctgacga tgaagcatat atgaggcgcg ggttgatttc ttgaagcgtt
     7381 tcccatgaaa accccagttt ttccattgta cctgggcgaa aattctcagc taatacatca
     7441 gcttgtttga gcatatttat aaatatactt ttatcgtaat cattctttaa atcaagaacc
     7501 acactctctt tgccatgatt aataaaactg taatagagtg actgtccatc cacataggga
     7561 ccaaatgtgc gggtatcatc accatgaccc ggcggctcaa ctttaattac ccttgcgccc
     7621 atattacaaa gaagttgagt tccgaaaggt ccattaagga catgtgtcat atcgataact
     7681 aataagcctt caaacggccc tttgctttca ttatttgtca tttgcttatg ccttgaaaaa
     7741 agccgaaggt gaaaagacac ttcggcattt atgtaaaatc aataacatga ttaattatga
     7801 aaccagtcga ctgacataaa tccacaaggg agccgtgacg acaaagccca gtacgctcac
     7861 agccaatgac gctgtaccag tacgggtgta gacattaaac cggctggcaa taataattcc
     7921 ggagaatgcc ggcggtaatg cgcctgccag taccatcatc tgcagatgtt cgctgttcaa
     7981 atgacatgcc ataccaacga gaagcagtgc cagtggcatc agaatcagct tcaggaaggt
     8041 gttataaaca atttcggcac tgaactcgaa tttatgtgca gccagagtca accccgcagc
     8101 gaataccgct acccctgagt tagccttcgc aatcagatta aaggttgggt cccatgctgc
     8161 cggaattttt accccaacca acaccaggat cgttgccaga acaggtgccc atactactgg
     8221 ctcctttgcc gcagaaatta atgcactcag attactattc ttcttaccat ccgctcctga
     8281 agaaggattc agcaaataca gaccaatagg aatagtaatt gcgttaacaa taatagaaat
     8341 aattgctacc actaaacctg ttgatacgga atcaccataa ataggatcga gaactgcaaa
     8401 ccccaggaat ccaatggtag gtgaacctgc aattaatgca catacagctg cttctgcatg
     8461 ggtacgttta aaaaatttgt agcaaccgaa ccaggagaaa aagaaacatc cgacaataac
     8521 cacaagtgat acaagggtca gacgagtgtc cgcaaaaatc atttccctgt ttgcccgagt
     8581 aatagataca aatagagccg caggaagcgc gtagtttaat accagtttat taaatgcgcg
     8641 ggcttgatct tctgaaaaag tttctcgtct gccgctaaaa taacccaaca gcatgattac
     8701 gataataggc aataaatcgc caataaaaaa tgttagcata atagtatttc cgataagggt
     8761 cccctcacca tcgcataatg aattggcaag aggagacgat gtcagtattt aattaccagc
     8821 gacttgtttt gggttaagtt tggtaatatg gccactttca gttcctgctg cagggtcgat
     8881 gaccacatta ataatggtcg gtttgcgcga ctgaataccg gtggttaaag catgacgaag
     8941 ttcatctgtc gtggtgacgt tatagccaac gccacgaaac gcatccatta atttgtcata
     9001 ccttgcatgg tgcaacagat ccgttggaga tggtgcacca gcgccactga gatcaacacc
     9061 gtctcctctg tagatgccgc cattattaaa aataacgatc gtcaccggca ggttatatcg
     9121 acaaatcgtt tcaatttcca tcccactgaa accaaaagca ctatcacctt caatggcgac
     9181 aaccggagaa ccagaagtca cgctagcacc gatggcatag cccataccga tgcccatgac
     9241 accccaggta ccacaatcca gacgacgacg tggtttatac atatcaataa tatttcgtgc
     9301 attatccagg gtatttgcac cttcattaac taaataaata tcctggttct cgcgcaatac
     9361 gtcgcgcaca gaacttaatg cattaaagta atttaatggt tgagtatctg tacttaattt
     9421 ttcatgcatt ttttgtgcat tttgctgctt gtggatattt aaaatatcgc gccataccag
     9481 tggagtcgta aatgtgtttt gtttcagttc tgccagcata ccttgcatac tggatgcaat
     9541 atcaccaatg actggcacag caatggggcg gttgctgtca atttcctgcg gttcaatatc
     9601 cagttgaata aactgtgtat ctgccgccca tcctttttta ccgtgtgcca ataaccaatt
     9661 cagtcgtgca ccaacaagca tgacaacgtc agcatttgcc agggcaaacg aacgcgcagc
     9721 tgccgcagaa agtggatgcg tatcttcaag gatccctttc gccatagaca ttggcaggaa
     9781 tggaatctga gcactttcaa taaattcacg aagctgttca tcagcttgtg aatacgccgc
     9841 gcctttgcca aggataatta atggccgttc agcttttgct aaaagcgaaa ttgcgctagt
     9901 gactgacttc gggcatggca ataatgctgg cgacggattt tcaactttaa caatcgtggt
     9961 taacgcttcg tctttttcca tcgtcgcggc caggacattt gctggcaaat caagataaac
    10021 tccgccaggg cgacccgata cagagacccg gatagcgcgt gccaatgcaa tgccaagatc
    10081 ctgcggatga ttaacgcgaa atgctgcttt ggcatacggt tttgccgcat tcatttggtc
    10141 cagctcttca taatcacctt gctgtaggtc gacgatcgcg cggtcgctgg agccgctaat
    10201 catgatcatc ggaaaaccat ttaccgttgc gttggccaat gccgtcaaac cattgaggaa
    10261 tcctggcgca gaaactgtca ggcagatccc cggtttttgg gtaagaaaac cgcttgccgc
    10321 agcggcatag cctgccgact gctcatgacg aaaaccaata taacgaatgc cttccgcctg
    10381 ggcatggcgt gccatatccg tcacaggaat acctacaaca ccataaatag tgtcaatatt
    10441 attctgtttt aatgcttcaa cgatgatatg cataccatct gtcatttgaa gttgatctga
    10501 catgattaat aacctcgaaa tttttataaa ttgggagcca gaagcccccg ttaatatcag
    10561 atggcgtggt tttgcttcat tgcagcaatt tcatcgtcgc tataacctag ctcctgcaat
    10621 acagcagcgg tatgttcacc taatagcggc gcagctttaa tatccggcgt aaaagcagag
    10681 aatttcattg gacaaccaac ggtcagatat tttccacgca acggttgttc cacttcaaca
    10741 acactgccac tttggcgcaa agagggatca agtgaaattt ctttcatact taaaaccggt
    10801 gcacaaggaa tatcaaactg agtcaaatag gccaccgctt catgtttatc aatagtgaca
    10861 gtgtattttt cgatttcagc aaaaatatcg aaaatatgtg gctgtcgggc atgggctgta
    10921 ctgtatgccg gatcggtaat ccattctggt ttgccgatgg ctttacaggt gttttcccag
    10981 ttttgctcct gaatagtgaa ataaatatag gcgttcggat cggtttccca gcctttacat
    11041 ttcaggatcc agccaggctg accgccacca cctgcattac caccgcgggg aactgcatca
    11101 ccaaatgtac cattcggata ctgcgggtat tcttccagat aacccaattt atcgagacgc
    11161 tgctggtcac gtaatttcac gcggcaaagg ttcaatacgg catcctgcat tgacatggtg
    11221 actcgttgcc cacgccccgt tttttcgcga tgcagcaaag cagcaagtaa accgatcagc
    11281 aaatgcattc cggtgttgct gtcacccaac gctgcagcgc ttaccagcgg cggaccatcc
    11341 caaaaacccg tagtggatgc cgcgccaccc gctgcctgag caacgttttc ataggctttt
    11401 acattcacat aaggcgaaca ctcatcaaac cctttgatcg aaccaaaaat cagacgtgga
    11461 ttgatttctt gaatatgctc ccaggtgaag cccatgtgat caatggcccc tggatgaaag
    11521 ttctcgacta agatatcagc ttcgcggatc agcttttcca ttacctcttt gccttccgct
    11581 gttttggtat ttaactcaat agaacgtttg ttactgttaa gcatggtgaa gtaaagcgca
    11641 tcgatatcag gaatatctcg cagctggtga cgcgttacgt caccaacgcc gggacgttca
    11701 attttaataa cgtcagcgcc aaaccaggcc agcatttgag tacaagatgg gccagattgc
    11761 acaccggtga aatcgagaac tttaattcct tgaagtggag ttgacatagc aatacctatt
    11821 agttaattaa atatgtgtta atttagcgtt tatttaaggc gtaatatctt ctataaataa
    11881 cccgcaaaat atcgtcgtag ctgcactcga tatttttaaa atcctgtatc aagtgctgaa
    11941 aatcatttca tcgccacttc caattccttt ctgcatggta agtagtgagg acgaaaagaa
    12001 gaatatgata aatattgatt cgtggtaatc agagagagtc gcgacatttg tgatgttatt
    12061 cttacaactg taagaagcgc cggtgattta caagtaaggg tatgattacg ctttttaaag
    12121 cgtaccataa aacataacat aataaatgat ttgtgatgac acgggattat tggggtttac
    12181 tttgaggaag ataaaatgcg tgtttataga accgcgagcg ccaaataatt ttatgaccac
    12241 aacctgtcgt attgtggtca taaaaaattg tcggctggag ggagttaaat cctttacctc
    12301 aatatactgc ctgtcagcat tagttcacaa acagtgcttc gctgtcgacg atgattccat
    12361 cttccgcacc tttcagcgcc aggtttgcct cgtagtattg ctttttatca agcagtttct
    12421 gcgcatcagc aagtgcatta cgagtctgtt taagcggcat cagatattgg ttttccatta
    12481 cgccaacacc ggccaggcga agttcttcca ttgcgccctt tttatcccct ttcgccattt
    12541 tttcattggc aatttttatc gcggcttctt tttcgggagt tgctacataa ctctcagaaa
    12601 ttccgacaga tgcattaatg acaatatatt ggtcatcatt gagattggtc tttttacctg
    12661 gcttagcgaa ctttgcccat tcggtactgt catctgacag caaagcggaa gcttcattgg
    12721 tcagttcttt tgccttttct ggatcgccat gaaacagagc caggcgcgct acctgcacgt
    12781 cacgcatcgc atataaacct tgttcagaga tacgctctgc tgctgccgtt ttctgggttt
    12841 gctgaacttg ctgagtttgc tgttgtgccg ctaccggtgc attatcagca gcccatacag
    12901 ttgaagatgc cagaattgct gtgaccatcg tagccataat taaacgtttc atacttacct
    12961 cattctttaa cagttttaga aaacgccgcc atcatattgg gtgagcatga aatgagaaat
    13021 cagagagcaa cggtcatttt gaaagggtta tcttacagtt gtagttatta tcgcaagaag
    13081 tattttagtc ttacaattgt aggaatctgc ttcctgtcgc agagaactat tcttatgtac
    13141 cacaaagaga tgtcatttat tatatcgatg taaatattga ttaattatat gtaaatacag
    13201 ccataacatc gtaggttctc agtgaaccat tttcaggata actttctatg aaagtaaact
    13261 taatactttt cagcttattt ttattggtct atattatggc atgcaatgtt tttgcatttt
    13321 ccatttctgg tggtgtaagt gagaggagct ataaagagac tgaaaaaaca tcagcgatga
    13381 cgaccacaca ctctacaaaa cttcagccat cacaggcgat tttgtttaag atgagagaag
    13441 atgcgccacc attaaacctc acagaagaaa tcacgcctac ttatccgaca aaggcgaatt
    13501 atcttattca tcctgtgcga tagcgtctgt cacatggcag gtgaacaatc atgtctgatg
    13561 ctgatgtgaa cacctgccca aatgagccat cctgacccac tatcgcctgc cataccccac
    13621 cataaagcga atcatcaagc cacaagccgg ggcaagaaac ataaatatct ccgtcactag
    13681 ttcattaccg ccagttaacc acatggtgaa gtaataaatg agtgcagcga ggatcaccat
    13741 gccgaagaaa gaagagaccg ccgataccac gttcagtacc tgaccgatgt agccgcttac
    13801 gcagacaata cacagggcga gaaacatcca taaataaatc atagctcaac ctcctgccgc
    13861 gctaaaaaac ctgtaacttc agtcacttta atagtaagac aatccttaac ctctggcaat
    13921 ttttgctaag catcaatgaa aacagcatgt taaatgcaag actgttgtgt acggaaaaat
    13981 atttactttg cacgattaat aatcagtagc tgaaagcagt cagcgagtga taaagtacag
    14041 ataccgcatt catgctcctc gtggttatgt cctgactagt ctttacacac tttacaggaa
    14101 ccattgtcgt acatgatggc ccaaccaatt gagtgtaccg ctgtcgacat ggaatcttct
    14161 gcgatacaac aattcgtatc tacagaaggt aactatgttt ccacaatgca aattttcccg
    14221 cgagtttcta catcctcgct actggctcac atggtttggg cttggtgtac tctggctttg
    14281 ggtacagctt ccttatcctg ttctctgctt tctcggcacg cgtattggcg caatggcgcg
    14341 accattcctg aaacgtcgtg aatctatcgc ccgtaaaaac ctggaacttt gtttcccgca
    14401 gcattctgcg gaagaacgcg agaagatgat tgccgaaaac tttcgttcac tcggcatggc
    14461 gctggtagaa accggcatgg catggttctg gcccgacagt cgcgtacgta aatggtttga
    14521 tgttgaaggg ttggataacc ttaaacgcgc acaaatgcaa aatcgcggcg taatggttgt
    14581 cggcgtccat tttatgtcgc tggaactggg cggccgcgtg atgggactgt gccaaccaat
    14641 gatggctacc tatcgtccac ataataatca gctgatggaa tgggtgcaga cccgtgggcg
    14701 catgcgctct aacaaagcga tgatcggcag aaataatctg cgcggcattg tcggtgcact
    14761 gaagaaaggt gaagcggtat ggtttgctcc cgatcaggat tatggtcgta aaggcagctc
    14821 cttcgcgccg ttctttgcgg tggaaaatgt cgccacaacc aatggcacct atgttctctc
    14881 ccgtctctct ggcgcagcca tgttgaccgt aacgatggta agaaaagcgg attacagcgg
    14941 atatcgtttg ttcatcaccc cagagatgga aggctacccg acagatgaaa atcaagccgc
    15001 tgcctatatg aacaagatta tcgagaaaga gatcatgcgc gcaccggagc agtacctctg
    15061 gatccactgt cgctttaaaa cgcgcccggt gggagaatcg tcgttgtaca tttaacaaac
    15121 tgctcttact ctttccctcc tcaaatcggc cataataacc tcatggtttt tatggctttt
    15181 ttcttaaggt tttttccttc gctgaatatc taattattac cgtcagaaat taaactgtcg
    15241 ccgatatgag acacttgtaa gttactaaaa gtaaataaga aaatgcctct tgtctcgttt
    15301 cagttttagg cgtacattag cgccgtctcg agcagtttag ctcagaattc tggggtgatg
    15361 cccacggtaa aatacagggc ttttaaggtc gctttgccca gtgtcaactc actgtatcag
    15421 gtgtaatgaa gtcattcagg cgtaacagca attacgcgga gagatgtaaa gtgaaatatt
    15481 tctttatggg catttctttt atggtcatcg tttgggccgg tacttttgcc ctgatgatct
    15541 aaaagcaaac tgtgcaatga aaaacaggag ccatcaggct cctgtttgca tttctgacga
    15601 cggtgtttat tccgcgtttt cgtgaatatg tttgctgctg gcgggtagta aaccgtcggc
    15661 acggaacatc gctttaatcc ctctgatggc ctgacgaata cggtcgcggt tttcaatcag
    15721 tgcaaagcga acatgcgtat caccatagtc gccaaagcca atccccggcg agacacagac
    15781 cttcgcttcg ttaagcaatt tcttggcaaa taccagcgag cccatggcag catacggttc
    15841 cggtattttc gcccagacat acatcgaagc cttcggcatt tcaactatcc agcccgcttc
    15901 atgcagccct ttaaccaata catcacggcg gcgtttgtac tgttcggcaa tgtcgcgcac
    15961 gcactgttga tcgccctcca gcgccgcaat cgccgccacc tgcaacggcg taaaggtgcc
    16021 gtaatcgtga tagcttttaa tacgagccag ggcgctgacc agcgttttgt tgccaaccat
    16081 aaagccgata cgccagcccg ccatgttgta gcttttcgac agcgtaaaga actcgactgc
    16141 cacatcgcgt gcacccggta cctgcatgat tgacggcgct ttccagccat cgtagacgat
    16201 atcggcatag gccaggtcgt ggaccaccag cacatcgtag cgtttcgcca gcgccacaac
    16261 cttttcgaag aactccagct ccacgcattg cgcggttgga ttcgacggga agccgaggat
    16321 catcatcttc ggtttcggat aactttcacg aatggcgcgc tccagttcgt tgaagaaatc
    16381 gacaccttcc accagcggca ctgagcgtac ctgcgccccg gcaatcaccg cgccataaat
    16441 atggatcggg taacttggat tcggcaccag caccgtgtca ccatgatcca gcgtcgccag
    16501 catcagatgc gccaggccct ctttcgaacc aatagtgacg atggcttctg attccgggtc
    16561 gatttcaacg tcgtagcgat cctgatacca gcgggaaatg gcgcgacgta accgcggaat
    16621 gccgcgtgaa gtggagtaac catgcgtgtc ctggcgctgg gccacagtac ataatttttc
    16681 gacgatatgc ggcggagtcg caccgtccgg gttacccatg ctgaaatcga taatatcttc
    16741 gccgcgccga cgcgcagcca ttttcagttc agcggtgatg ttaaaaacgt agggcgggag
    16801 acgatcaatg cgcgtaaagc gacgttcagg gcgagtgtca gccataattt cctcagatta
    16861 acgttagcgc ccggaccgtc cgagcgacgc tgccacaatg gtggctgttt tgaaaatagc
    16921 ctgattaatt tctgtctgtc cagcgtttta gaacattttt atcaatggct ttaaatgagg
    16981 aactccactt caaaaacagg aaatttttct acgctaacaa gaaaattacc accaatttta
    17041 ttaacattat aatttcaata aatttaccag ccagaagtgc attatcaaga gatacattgc
    17101 taatacacca tttatctttc aatccccttt taaatttatg gtttttcctc aaaaacggcc
    17161 tgctggtcat cagccttcag gttacctatc atagaggttt aatccttatt cagagtcacc
    17221 cgtgcacgaa atattcaaca tgctgctggc ggtcttcgat cgggcagcgt taatgcttat
    17281 ctgcctgttc tttctcatcc gtatccgcct gtttcgcgag ctgttgcaca agtcggcaca
    17341 ctccccaaaa gagttgcttg ctgttaccgc cattttctcg ctgttcgccc tgttcagcac
    17401 ctggtccggc gttcccgtag aaggctcgct ggtgaacgta cgtattatcg cggtgatgtc
    17461 cggcgggatt ctgtttggcc cgtgggtagg catcattacc ggcgtgattg cgggtattca
    17521 ccggtattta attgatatcg gcggcgtgac ggcgatcccc tgctttatca ccagcattct
    17581 ggcgggttgt atatcaggct ggataaacct gaaaatcccc aaagcacagc gctggcgcgt
    17641 cggtattctc ggcggcatgt tgtgtgagac gttgaccatg attctggtaa ttgtctgggc
    17701 accaactacc gcgttgggga tcgatatcgt ctctaaaatc ggcattccaa tgatcctcgg
    17761 tagcgtctgt atcggcttta ttgtgcttct ggtgcaaagc gttgagggcg aaaaagaggc
    17821 cagcgccgcg cggcaggcca agctggcgct ggatatcgcc aacaaaacgc taccgctgtt
    17881 tcgccatgtc aatagcgagt cattacgcaa ggtctgcgaa attatccgcg atgacattca
    17941 cgccgatgcg gtggcgatta ctaataccga tcatgtgctg gcctatgttg gcgtgggtga
    18001 acataactat cagaatggcg atgacttcat tagcccgact acccgtcagg cgatgaatta
    18061 cggaaaaatc atcattaaaa acaatgatga agcccaccgc acaccagaga ttcattccat
    18121 gctggtgatc ccattgtggg agaaaggcgt tgtgaccgga acgctgaaaa tttactactg
    18181 ccacgcgcat cagataacct cgtcattaca ggaaatggcg gttggtctgt cgcaaatcat
    18241 ctccacgcaa ctggaggttt cacgcgccga gcagctacgt gaaatggcaa ataaggcaga
    18301 gcttcgcgcc ctgcaaagca aaattaatcc ccattttctg tttaacgctc tgaacgctat
    18361 ttcatcgtca atccgtctga atccggatac cgctcgccag ttgatcttta atctgtcgcg
    18421 ttatctgcgc tataacattg aattaaaaga cgatgagcaa atcgatatca aaaaagagct
    18481 gtatcaaatt aaagactata ttgccattga gcaggcccgc tttggtgaca agctgacggt
    18541 tatctatgat attgatgaag aggtgaattg ctgcattccc agcctgctga tccagccgtt
    18601 ggtggagaac gccattgtcc acggtattca gccttgcaaa ggtaaaggcg ttgtcaccat
    18661 cagcgttgca gagtgcggaa atcgggtacg cattgcggtg cgagataccg ggcacggcat
    18721 cgatccaaag gtgattgagc gggtcgaagc gaatgaaatg ccgggcaata aaattggcct
    18781 gctgaatgtc catcatcgcg tgaagttatt gtatggcgag gggctgcata tccgccgcct
    18841 ggagccgggg acggaaattg cgttttacat tcctaaccaa cgcaccccag tcgcctcaca
    18901 ggctacgtta ttgctttgag ccggagtgat attgtgaaag tcatcattgt tgaagacgaa
    18961 ttcctggcac aacaggaact gagctggcta attaaagagc acagccagat ggagattgtc
    19021 ggcacctttg acgacggtct ggacgtgttg aagtttttgc agcataaccg cgtcgacgcc
    19081 atttttctgg atatcaatat tccgtcgctg gatggcgtgt tgctggcgca aaacatcagc
    19141 cagttcgccc ataaaccgtt tattgtgttc atcaccgcgt ggaaagaaca tgcggtagaa
    19201 gcgtttgaac tggaggcgtt tgactacatt ctcaaaccgt atcaggagtc acgtattacc
    19261 gggatgctgc aaaaactgga agcggcctgg caacaacagc agaccagcag tacgcctgcc
    19321 gcgacggtaa cgcgtgagaa tgacaccatt aatctggtga aagatgagcg aataatcgtc
    19381 acgccaatta acgatatcta ttacgccgaa gcgcacgaga aaatgacctt tgtctatacg
    19441 cggcgtgaat cctacgtaat gccgatgaac attaccgaat tttgcagcaa actgccgccg
    19501 tcgcattttt tccgctgcca tcgctcattt tgtgtcaatc tgaacaaaat acgcgaaatc
    19561 gaaccgtggt ttaataacac ctacattctg cgactgaaag atctggattt tgaagtgccg
    19621 gtcagccgca gcaaagtgaa agaatttcgc cagttaatgc atctttaatc gggaactttc
    19681 atgaaagcac caggcttgcc cgccgatcaa caatttttcg ccgatctgtt cagcggcctg
    19741 gtgcttaact cgcaactact ggggcgcgtc tggtttgcca gccagcctgc ctcgttaccg
    19801 gtgggcagtt tatgtattga ttttccccgt ctggatatcg tgctgcgcgg cgaatacggc
    19861 aacctgctgg aagcaaagca gcagcgaatg gtggaaggag aaatgctgtt tattccggca
    19921 cgcgcggcta atttaccgat caacaacaaa ccggtgatgc tgttaagcct ggtgttcgca
    19981 ccgacctggc ttgggttatc gttttacgat agccgcacca cgtcgttgtt gcatcctgct
    20041 cgccagatcc agcttcccag cctgcaacgc ggtgaaggtg aagcgatgct taccgccctc
    20101 actcatctta gccgttcgcc gctggagcag aatatcattc agccactggt gttaagtttg
    20161 ctgcatcttt gccgcaacgt ggtgaatatg ccgccgggta attcgcaacc gcgcggcgat
    20221 tttctctatc acagcatttg taactgggtt caggataatt atacccagcc gctcacccgc
    20281 gagagcgtgg cgcagttttt taatatcacg cccaatcatc tgtcaaaact gtttgctcag
    20341 catggaacga tgcgttttat cgagtatgtg cgttgggtgc gaatggcgaa ggcgaggatg
    20401 attttgcaga aatatcatct gtcaattcat gaagtggcac agcgttgcgg ttttccggat
    20461 agcgactatt tttgtcgcgt tttccggcgt cagtttggtc tgacgccggg agagtacagc
    20521 gcccgttttc agggctaacg tcagaaggtt aattctgttt ccagcagcgt caggatactt
    20581 tgtgcatctt gcgcggcaaa caacgactgg cggaagtttt tattcaccag tttacgcgcc
    20641 agctgcgaga agactttcac atgattcatc ccttcgttag cacccagcgt cagcatgatc
    20701 accagttcga cttcgcccat ttctgactgc cagtcaatcg gtttcgccag ccgggcaatg
    20761 ctgatactgg aatgacggat ccactgagat ttggtgtgcg ggatcgctac gccaaaacca
    20821 acgccggtgg taacaatctc ttcccgctgc cagacatctt cttccagctc aaacggatgt
    20881 tcagtgcgcc cgttaacgcc gaggttgccg cacaggaact ggatcgcctg ctctttattg
    20941 ctaaaatcct gatcaacaaa gatattctcc agcgccagca gtgggcgaac gtcttcttcc
    21001 ggcgtaaagg cggtgagtaa cgcttcaatt tcctgcgcac tgcggcattc acatgcctga
    21061 cgcgccagtt cccgacacgc ctcgctatcc agttgacgaa gctggctttt caccgccgga
    21121 atacgcgggc tactcatact cagctcatcc aggcccagcc caagcagtag cggcagataa
    21181 cggctttcac cgcccagttc accgcaaatg cctacccatt tgccccgctg atgcgcagtg
    21241 gtaactattt gctgcaacat gcgcaggaac gatggcgtaa tcgggttata tagcggcgat
    21301 acgcgcgggt tattacgatc gaccgcatac agatactggg tcatatcgtt ggagccgata
    21361 ctgaagaaat cgacctcatc gcagaagtgg tcgatgatgt agcacaccga cggcacttcc
    21421 accatgatcc caagcgtaat cgtctctgca tgacgcaggc catcgcgctt aagctcaacg
    21481 atcgcttttt ggatctcgcc cttcacccat aagatctgat cgagactgtg gaccatcggg
    21541 atcatcaact gggcgttgcc aaaactggcg gcgcgcaaaa tcgcccgtag ttgagtgcgg
    21601 aacaggccag caaattccgg gtaaatacgt accgcgcgat agtcgaggaa cgggttctct
    21661 tcctggggaa tattcagata aggaatgctt ttatcgccac cgatgtccat tgtgcggaag
    21721 ataatcggct tgtcgcccgc tgccagtagc acctgctggt aggcttcaaa ttgctcctgc
    21781 tcgtccggcg cgctgtcgcg atccatatag agcatttcag tacggaacag tccaacgcct
    21841 tccgcgccgt tggcaaacgc gcctggcgct tccagagcgg tgccgatatt cgccgcaata
    21901 tcaatacgct tgttatcacg ggaataggcc agctgcgcgg cggcctgcgc ctgttgtttt
    21961 tggcgtttat ccgccagcgt ctgtgcgacc tgataataac cgctcaccgc gtcattcggg
    22021 ttaatcgcca gcacgccgca ctgggcgtca agcaccgccg gttgcccggc ataacgggca
    22081 atcgcatcca gcggcaggcc gctcagtacc gggatcgccg aggcgcgggc cagaatcagg
    22141 gtatgcgagg tgcgcccagt tttctccaga atcatgcctg caagattttt taaatcgagg
    22201 ctcaaaaact ggcttggggt taaatcctcg gctaccagaa tagtcggttt ttccagcacc
    22261 agattgttgc gcggcttcag ttccggccag gtgatatgca gcaactgttc gctaatatcg
    22321 cgaatgtcgc taacacgttc gcgcagataa tcactggcag aagcagaaag tttggcgcaa
    22381 acctgctcca tattgctgat gatcgccgcc cccagccctt gatgctgttc tgtcatcaga
    22441 cgacggatat tgcccgcgaa ttcatcatcc tgaatcagcg acaaatgggc gctaaggata
    22501 gttttgcttt cgccgtcact ctcacgcaat tgctggttca gttgctcggc aagcgttgca
    22561 aggctgtgct ccagccgggt ggaatcttgc gcactggcgg ggattgcccg ataactgtcg
    22621 aggctgtcgc tttgtaacag ggtcagcgta cccacgccga cgctgcttgc cagcacattg
    22681 ccgtacagta aatccgggtt caggcggctt aatgaacgcg gcagcggatg cgccgtcagt
    22741 tctgcctgcg ttggctggat gctgtcgctg tcgataaagc gcacctggat gtactcttcc
    22801 aacacgcgct gcgcctgctc ttcatcgctg ccgctaatgt tcaggctgca actgtcatta
    22861 aataaagtac cggtgccaat cagcgccagc gagcttttcg catcggcctt tgcgttctgg
    22921 cgatggttga taaaggtgat ttcgctttgc cactggctgc actgttcttt aagttcccac
    22981 gccggacgag catgaagacc gtttggcaaa ggacagagaa attgaatcgt taacataacc
    23041 gctccgggtt aatcaggaga tcatctgaaa tccgtcagtt gaacaaccgt ctcacgcgta
    23101 agacgtttaa taagggcaga taaaagctgc tgcatctgca aaatgtcacg gcaatcggca
    23161 atcgatgcgg cgcaatgtcc gtggcgggtt gctggcccca tcaccactgt aggcacgccg
    23221 gtgccggtta agtgcaccgc tccgccgtcc gtgccgccgt tgctgaacat atctgcctgt
    23281 aacggcacgc caatttctgc cgccacggtt tcgatccacg cggtaagttt tggcggcgca
    23341 atcagcgact tgtcgcttaa caccagcatc ggcccgttac aaatctgacg atggttagcc
    23401 gcgccataat caaagttttt cgcccagcag gcggtatcaa gcacaatggc gacatccggc
    23461 gacaccgcgc gggtggcagt ttgcccgccg cgtaccccca cctcttcgct ggaactggcc
    23521 accagccaca cttccgcagg cagttcagcg ctgtgtagtt cgcgcagtaa cgtcaccagc
    23581 aggtagcaac cgaggcggtc atcaaaggct ttccccatca cccgctggtg agggagaacc
    23641 tgaaaagttg tatcaaaggt gacgcgatcg cctggacgaa ttcccgcctg catcacttcg
    23701 tcatacgagc gcgtaccaat gtccacgcgc atggcgctga cgtcattccc ctgccggtcg
    23761 ccgtcaagca ggcctggaat tttgcactct tcacgggtgg tgatgcgcac cggctgcagc
    23821 tggcgggcag ccatgcgcac gttgccaacc ggcagcacat caattgcccc ttcgcgggag
    23881 atgctgcgca ccataaatcc cacttcgtcc atatgggcgc agatcatcac cttcggccct
    23941 gtcgattcat tgaggcggat cagcaccgat cccagaccat caaatcgcac ttctttttgc
    24001 aggcgatccg cttcttccag caggatctgc cgcacttcct gttccgagga ggcgatcgca
    24061 tctgcctcgc tcaacgcttt taatagcgat aaatccatta tgcctctccc gtgagcaaca
    24121 ctgttttcgg catggcgtag agcacttctg cgccttgcgg ggtaaccagc acaacatctt
    24181 cgatgcgcac gcccccttgc cctggcaaat aaatccccgg ctccacggtc agcaacatgc
    24241 ctggctgtag cgtcgtggtg tcccgcggtg aaaaacgcgg atcttcatgg acttcaatgc
    24301 cgatagcgtg accagtgtta tgaccgaaat agtcgccaaa tccagcctcg gtaatgaccc
    24361 ggcgcgcggc gtcgtcaatc tgctggcagc gcacgccggg gcgaattgca gagattgccg
    24421 cgagctgtgc ctgcaggaca atctgataga cgtcaaacag cgggtgagat tcggcgctca
    24481 ccccttcgcc attcaccagc aaggtgcgcg tcatatcaga gcagtagccc tgatacagcg
    24541 caccaaaatc gagagtgaca aactcgcccg ctgcaacaat cttgtcgctg gctttgccgt
    24601 gcggcagcac cccgcgccag ccgctggcga caatggtgtc aaaagaggct ttttctgcgc
    24661 cctgctggcg cataaaccac tccagttcag cggctatctc gcgctcgctc atccccgcct
    24721 gaataaagcg gcgaatatgc tccgctccgc gatcggcaat cccacaggcg aggcggatta
    24781 tctccacctc ctctggcgtt ttgatttgcc gcagcacatc cggcgtggcg ctaaccagtt
    24841 tcgcattgag ttcagacttc cagcggtgcg cggtttccca actcacctgc tggccttcaa
    24901 aaccgagcgt ttgcaactgc tcatcggcaa tgatttgatt gacgatagtg gtaagcgtgt
    24961 tcgtcgcgtc aagcaaatgc agctggtagc cttgcgcacg ggcttccacc tcaacgaaat
    25021 agctcgaatc taccagaatg tgcgcacttt cacggctaat aaccacatag cctgatccgg
    25081 tggagatccc cagatgcggc tgtttgttct gccgtgagga gagaagcact gcatccagtt
    25141 gttgctcctt aagccagtcg cgcagcgaag cgagtaatgt catctggatt ttccttctta
    25201 caggctatcg attaacaatt tgcctttacg gaacatcatc agacgcagga acaccaccat
    25261 cagcgcagtt atgactgccc ccagcgcgat ccccgccata tagacgccaa ggttagtgac
    25321 cagcggccat gcccagatag cggattccgg gaaccattgc actgcgccca gccagacagc
    25381 ggcggttgag ccgacaatcg cgccgaccat atacgacgga atggcggtga tggggctttc
    25441 cagcgcaaac ggaatcgcac cctcactaat ccccataaag gcaaggaaca tcgcggtctt
    25501 accctgcgga taaagttgag cgttgaacag gcgtttgccg gttaaacgac ggtcaataat
    25561 ggtcgccagc cccagaccaa tcggaggaat aacgatagcg atagaacgag cggtgaccgg
    25621 taatacgtgg tcagtggtaa agctgaaggc aacgaaacct gcggctttgt tgatcgggcc
    25681 accaaggtcg attgctgtcg ccgcggcgat ccccattgcg tacatcagcg cgcccttctc
    25741 acctgcggca gtcagcacgg tacggataac gccgttgatc cagccgccaa agggggtaat
    25801 gacgtagtac atcgccagca tgacaaaaat ggcggaaaga atcggtagca aaaatgtggt
    25861 tttgaacgct aacaggaaat ccggtaactg gattttctgg ttcatccact tcacaaggta
    25921 acccgcgacg atagaaataa tcagcgcacc aatgaaggtg gacggcaccg gcgaagaggt
    25981 cgcccattgc atagtgctgg gatcgaagtt cagcagttgc gtcggctggg tagacatcaa
    26041 gccgccgata aaaccagccg ggaacgccag tttgccaccg atagagttcg ccacaaaagc
    26101 agcaaacatc ggaatggcaa aaccaaacaa cacgccgccg aaggactgcg acagccaggc
    26161 aaatttcagt aaggagaggt cgaaaccgga gaatttcccg ctattaagag catccatgat
    26221 gccgatctca gcaggaattt taagccagct ataagcaatc agctggctga aagcgaggat
    26281 cacaccgccc ataattaagg tcggcaccat gcgtgaaatc cctgacatca catgctgcgg
    26341 gagttcaccc cagaagctgc ttttagaggc ctgcggattc ttaactgccg ctgccgcacc
    26401 ggatgcgcca ggcacaacgg ttgcactgcg ttttttaatg gccataacaa ttatccctgt
    26461 cgaatattac tgttgttcag aggcaatcat ctcttcgatt tctttgatga tgcccgcagc
    26521 gtttttaatt gcgtcctgca aagtgatttc ataaacgtcg cgtgattcga aacgctcatt
    26581 atcttccggg gtaactgcca cggagtggat gatgatggtt gcttcggcga tatcctgcgc
    26641 cgtcaggcga ttctggatac cgtccgcgcc ctgggtttca attttcactt cataaccggc
    26701 ttctaccgcc gcttcttcca acgcctgagc ggccataaag gtgtgagcca ggcccattgg
    26761 gcaggcacat aaggcaatca gtttcttact catcgttaat tcctcattca gtgatagttg
    26821 acaagtgaaa ttatgaacgt gcaaaacaaa tcgccgttac cagacaaata atgcatttac
    26881 tggagaatta atcccgctgt acggaagtgt gacgcaggtc ggccttgtga ggggacacaa
    26941 agaaaaattg aattaactgt atgatttaaa cgattatcgg gagagttacc tcccgatata
    27001 acaggaagga tttacagaat gtgccctaag gtctggcgta aatgtgcacc ggaaccgaga
    27061 agacccggat tgtcatggac gatgagatac accggaatat catggacata ttctttaaag
    27121 cgccctttat cttcaaatgc agcacggaaa ccagaggctt tgaagaactc aaggaagcgc
    27181 ggcacgatac cgcccgcaat aaacacgccg ccaaatgtcc cgagattgag cgccagattg
    27241 ccgccaaaac ggcccataat gacgcaaaac agcgacaacg cgcggcggca atcggtacag
    27301 ctgtcagcca gcgcgcgttc ggtaatatct tttggcttga gattttctgg caggcggttg
    27361 tcagctttca caattgcgcg atacaaattc accagcccag ggccagaaag cacgcgctcc
    27421 gccgaaacat gaccaatttc cgcacgcaat atttcgagga taatggcctc ttcttcacta
    27481 ttcggcgcaa aatcaacgtg accgccttcg cccggcaagc ttacccaacg cttatcgaca
    27541 tggaccagat gcgcaacccc aagccctgtt ccggcaccgt aaaccgcaat aggtttacct
    27601 tctaccggtt ctgcgccacc aaactgaatc agatgctctt ttttcagcat cgggatcgcc
    27661 atcgatacag cggtaaaatc gttaataatt tccagatggc taaaaccgag attctttttc
    27721 atttcggcaa ttgagaacgc ccaggtatgg ttggtcattg ccacccagtc accggtaatt
    27781 gggcaagcga tggcaataca gccgtctttc acctcgacct tatgttcttc aagataaacg
    27841 cgaatgaccg cttcgaggct ggggtaatca agccctgaat aggtcttagc ctgcgagatt
    27901 tcaccactgg caatatcaca cagagcaaga cgtgcgttgg tgccgcccac atcaccgact
    27961 aatgcatact ttatcattct tcaactgctc cgctaaagtc aaaataattc tttctcacac
    28021 tgtaaatacc tgggggcata acaacaacgc cggaaaggca ggctccctgt aaatatcgat
    28081 ctgggtcaca catttacttt atcgtttcag caccaattgc agcgatgcct ttttgcaagc
    28141 tgggcaaact aagtatctga ccccgcataa ggaatagaac atgctccatc cgcgagccag
    28201 aaccatgttg ttattatcgc tccccgccgt ggcaattggg attgcgtcca gtcttattct
    28261 gattgtggtg atgaaaatcg cctcggtatt acagaatttg ctctggcaac gactgccggg
    28321 aactctgggg atagcccagg attcacctct ctggatcatc ggtgtattaa cgctaacggg
    28381 tattgcggtg gggttggtta tccgtttcag ccagggtcat gccggaccag accccgcctg
    28441 tgaaccgctg atcggcgcac cagttccgcc ctctgcgcta ccaggactta tcgtagcatt
    28501 aattctcggt cttgctggcg gcgtcagcct ggggccggaa catccgatca tgaccatcaa
    28561 tatcgccctt gccgtcgcca ttggcgctcg tctgttaccg cgcgtcaacc gaatggagtg
    28621 gactatttta gcctctgccg gaaccatcgg cgcgctgttt ggcacacctg ttgcagcggc
    28681 gttgatattt tcgcaaacct taaatggcag tagtgaagtt ccgctatggg atcgtctctt
    28741 tgcgccgtta atggcggcag cagctggtgc actaactacc ggattatttt tccatcccca
    28801 tttttcactg cccattgcgc attacggaca gatggaaatg accgatattc tcagcggtgc
    28861 aattgtcgcg gcgattgcca tcgcagcagg gatggtcgcc gtatggtgct taccacggtt
    28921 gcacgcgatg atgaatcaaa tgaaaaatcc ggtgctcgtg ctgggtattg gcggatttat
    28981 tctcggtatt ctgggggtta ttggtggacc agtttcgctg tttaaagggc tggatgagat
    29041 gcagcagatg gtggcaaatc aggctttcag caccagcgat tactttttgc tggcggtaat
    29101 taaacttgcc gccctggtcg ttgctgccgc cagtggcttt cgcggtgggc gaatcttccc
    29161 ggcagtgttt gtcggcgtgg cattagggtt gatgctgcat gagcacgtac ccgccgtacc
    29221 agcggcaata accgtttctt gcgctattct cggcatcgtg ctggtggtaa cacgcgatgg
    29281 ctggttaagt ctttttatgg cggcggtcgt tgtacccaat accacattgc taccgctgct
    29341 ctgtatcgtc atgcttccgg catggctgtt attagcaggt aagccgatga tgatggtcaa
    29401 tcgtccgaag caacagccac cccacgataa cgtttagcaa aaaatgctct cctttatgat
    29461 ttaagagtta tggcgatgat acggggcttt acctccccgt aatattgcgt taacaggccg
    29521 ctgacggctt cgatcgtgaa ggagaataac aatgttcagg tcactgtttc tggcggccgc
    29581 cctgatggca tttaccccgc ttgcagcaaa cgcaggtgaa atcaccctac tgccatcaat
    29641 caaattacaa attggcgatc gcgatcatta cggtaattac tgggacggtg gtcactggcg
    29701 cgaccgtgac tactggcatc gcaattatga gtggcgcaaa aaccgttggt ggcgtcatga
    29761 taatggctat catcgtggct gggataagcg taaagcgtat gagcgtggct atcgtgaagg
    29821 ctggcgcgat cgtgacgatc atcgcggaaa aggccgcgga catggacacc gccattaaac
    29881 acattcgcca atggagcaca atgcctgatg cgacgctagc gcgtcttatc aggcctacaa
    29941 accggatctt taacgcgtag ggcggataag gcgtttacgc cgcatccgcc aatagtgcca
    30001 gatgcgacgc tcattcaact acaatcccag cgccgtcccc accaacaacc agatattcag
    30061 cgccacgacc agcactacaa tcacccagcc tgtctgtttt acgcgtttgc tgttcaccag
    30121 atcgcccatc aacttgctgt cactggtgaa aatcagcagt ggaaccagcg ccagggcgat
    30181 accaaaactt aacagcacct gactcataac cagaatccgt gtcggatcta atcccatcag
    30241 aatgacaata aatgacggca gcatggtgac tgtacgacgc acccacagcg ggatatggaa
    30301 gcgaatgaac ccctgcatca ccacctgccc tgccagtgtc cccactaccg ttgaggacag
    30361 tccggcagca accagactta acccaaagac cgttgccgca gcatggctta acagcggttg
    30421 cagcgtcaga taagcctcat caagatcggc aacaccagta tgaccagaaa agtggaacgc
    30481 cgccgcagct gtagccatca tcgccagatt gacaaaaccg gcaatagtca tggcgatagc
    30541 cacatcccat ttggtggcgg aataacgttg ttgacgcgaa ccgccatgta aatgctgagt
    30601 gagcgaggag tgcaaataaa tcacatgcgg cataatcgtc gcccctaaca cgcctgctgc
    30661 caggaagacc gcttccgaag taggtaaact cgggatcacc attcctttac ccagctgcgc
    30721 caggttaggc tgggagaaaa tcaactcgac aatgtaagcc gcggcaacaa acaacagtaa
    30781 cccgccaatc actttctcca gcggtttttg cccgcgacgt tgcagcatta aaatcaggaa
    30841 agtcgcgatc cccgtcagca ccgcgccctg caacaacgaa acaccaagaa tgagtttaaa
    30901 accgatcgcc gcaccaataa attccgccag gtcggttgcc atcgcaataa tttctgcctg
    30961 aacccaatag aaccacacta cgggacgcgg atagtgatcg cgaatctgct ccgccagatt
    31021 tttaccggtg gcaatcccta gtttggcaga gaggatctga atcagcatcg ccatcaggtt
    31081 ggcccaaacg acaacccaca gtagctgata gccgaagctg gcacccgcct gaatattggt
    31141 cgcaaagtta ccgggatcga tataaccaat cgccgcaatg aacgcaggtc ccattaatgc
    31201 gagcctcatc ttgcgcgccg cccgtccgct gctactctca acgcgatagt tcgtcatctt
    31261 gtgcctctaa aacatagcct ttgctatgtt tcatgctatg ataaacgaga atgattatca
    31321 aattcattta aatggattgt ggtgatttct gtgatagacc aggattatga ctacgaaaag
    31381 attgatgact tgaatgttat ggttatgttt aatgttagca catttacata actttcagct
    31441 tccatacaca acatagcaga aatgtatgac agatcactat ttttgaagcc tgtcacagga
    31501 cgtcattata gtgtgtgtca gatctcgttt tccttaacca tgttacatag aatgtgcacg
    31561 gaaatttaac ctgcctcata tttggagcaa atatggaccg cgtccttcat tttgtactgg
    31621 cacttgccgt tgttgcgatt ctcgcactgc tggtaagcag cgaccgcaaa aaaattcgta
    31681 tccgttatgt tattcaactg cttgttatcg aagtgttact ggcgtggttc ttcctgaact
    31741 ccgacgttgg tttaggcttc gtgaaaggct tctccgaaat gttcgaaaaa ctgctcggat
    31801 ttgccaacga agggactaac ttcgtctttg gtagcatgaa tgatcaaggc ctggcattct
    31861 tcttcctgaa agtgctgtgc ccaatcgtct ttatctctgc actgatcggt attctccagc
    31921 acattcgcgt gttgccggtg atcatccgcg caattggttt cctgctctcc aaagtcaacg
    31981 gcatgggcaa actggaatcc tttaacgccg tcagctccct gattctgggt cagtctgaaa
    32041 actttattgc ctataaagat atcctcggca aaatctcccg taatcgtatg tacaccatgg
    32101 ctgccacggc aatgtccacc gtgtcgatgt ccatcgttgg tgcatacatg accatgctgg
    32161 aaccgaaata cgtcgttgct gcgctggtat tgaacatgtt cagcaccttt atcgtgctgt
    32221 cgctgatcaa cccttaccgt gttgatgcca gtgaagaaaa cattcagatg tccaacctgc
    32281 acgaaggtca gagcttcttc gaaatgctgg gtgaatacat tctggcaggt ttcaaagttg
    32341 ccattatcgt tgccgcgatg ctgatcggct ttatcgccct gatcgctgcg ctgaacgcac
    32401 tgtttgccac cgtgactggc tggtttggct acagcatctc cttccagggc attctgggct
    32461 acatcttcta tccgattgca tgggtgatgg gtgttccttc cagtgaagca ctgcaagtgg
    32521 gcagtatcat ggcgaccaaa ctggtttcca acgagttcgt tgcgatgatg gatctgcaga
    32581 aaattgcttc cacgctctct ccgcgtgctg aaggcatcat ctctgtgttc ctggtttcct
    32641 tcgctaactt ctcttcaatc gggattatcg caggtgcagt taaaggcctg aatgaagagc
    32701 aaggtaacgt ggtttctcgc ttcggtctga aactggttta cggctctacc ctggtgagtg
    32761 tgctgtctgc gtcaatcgca gcactggtgc tgtaagacca tacataaaaa agccggggat
    32821 aattcc
//
LOCUS       DADLFA010000026        32489 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000026 DADLFA010000000
VERSION     DADLFA010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32489
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.026"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            197..991
                     /gene="panB"
                     /locus_tag="JQH65_001033"
     CDS             197..991
                     /gene="panB"
                     /locus_tag="JQH65_001033"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HAY6138520.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTASVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1003..1854
                     /gene="panC"
                     /locus_tag="JQH65_001034"
     CDS             1003..1854
                     /gene="panC"
                     /locus_tag="JQH65_001034"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HAY6138521.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(2146..3042)
                     /locus_tag="JQH65_001035"
     CDS             complement(2146..3042)
                     /locus_tag="JQH65_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HAY6138522.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQGKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            3316..3696
                     /gene="panD"
                     /locus_tag="JQH65_001036"
     CDS             3316..3696
                     /gene="panD"
                     /locus_tag="JQH65_001036"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HAY6138523.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3700..4900)
                     /locus_tag="JQH65_001037"
                     /pseudo
     CDS             complement(3700..4900)
                     /locus_tag="JQH65_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899461.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
     gene            complement(4995..5435)
                     /locus_tag="JQH65_001038"
     CDS             complement(4995..5435)
                     /locus_tag="JQH65_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAY6138524.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5540..6310)
                     /locus_tag="JQH65_001039"
     CDS             complement(5540..6310)
                     /locus_tag="JQH65_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY6138525.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLATTFGVLVVFIVVFYLICWSLIQRGRGLRS"
     gene            complement(6307..7233)
                     /locus_tag="JQH65_001040"
     CDS             complement(6307..7233)
                     /locus_tag="JQH65_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY6138526.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKLETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7342..8004
                     /gene="can"
                     /locus_tag="JQH65_001041"
     CDS             7342..8004
                     /gene="can"
                     /locus_tag="JQH65_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HAY6138527.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPHFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8045..8581)
                     /gene="hpt"
                     /locus_tag="JQH65_001042"
     CDS             complement(8045..8581)
                     /gene="hpt"
                     /locus_tag="JQH65_001042"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAY6138528.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8787..11177
                     /locus_tag="JQH65_001043"
     CDS             8787..11177
                     /locus_tag="JQH65_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone-dependent
                     dehydrogenase"
                     /protein_id="HAY6138529.1"
                     /translation="MAINNTGSRRLLVTLTVLFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPTEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPKLKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11224..12774)
                     /gene="cueO"
                     /locus_tag="JQH65_001044"
     CDS             complement(11224..12774)
                     /gene="cueO"
                     /locus_tag="JQH65_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HAY6138530.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQCGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12940..13287
                     /locus_tag="JQH65_001045"
     CDS             12940..13287
                     /locus_tag="JQH65_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HAY6138531.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13393..14259
                     /gene="speE"
                     /locus_tag="JQH65_001046"
     CDS             13393..14259
                     /gene="speE"
                     /locus_tag="JQH65_001046"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HAY6138532.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPSGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14275..15069
                     /gene="speD"
                     /locus_tag="JQH65_001047"
     CDS             14275..15069
                     /gene="speD"
                     /locus_tag="JQH65_001047"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HAY6138533.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15106..15468)
                     /locus_tag="JQH65_001048"
     CDS             complement(15106..15468)
                     /locus_tag="JQH65_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HAY6138534.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15643..18240)
                     /gene="acnB"
                     /locus_tag="JQH65_001049"
     CDS             complement(15643..18240)
                     /gene="acnB"
                     /locus_tag="JQH65_001049"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HAY6138535.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18595..20448
                     /locus_tag="JQH65_001050"
     CDS             18595..20448
                     /locus_tag="JQH65_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HAY6138536.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAAVETQSTQ
                     LAVSDAAAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDALLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSNEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTASPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FIDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVRERMQTNPQIQQRREAARERIQPASPEQRQ
                     AVREKMQTNPQIQQRRDAARERIQSASPEQRQVFKEKVQQRPLNQQQRDNARQRVQSA
                     SPEQRQVFRERVQESRPQRLNDSNRTARLNNDQRSAVRERLSERGARRLER"
     gene            complement(20519..21943)
                     /gene="lpdA"
                     /locus_tag="JQH65_001051"
     CDS             complement(20519..21943)
                     /gene="lpdA"
                     /locus_tag="JQH65_001051"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAY6138537.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22268..24160)
                     /gene="aceF"
                     /locus_tag="JQH65_001052"
     CDS             complement(22268..24160)
                     /gene="aceF"
                     /locus_tag="JQH65_001052"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAY6138538.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(24175..26838)
                     /gene="aceE"
                     /locus_tag="JQH65_001053"
     CDS             complement(24175..26838)
                     /gene="aceE"
                     /locus_tag="JQH65_001053"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HAY6138539.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHSI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(26999..27763)
                     /gene="pdhR"
                     /locus_tag="JQH65_001054"
     CDS             complement(26999..27763)
                     /gene="pdhR"
                     /locus_tag="JQH65_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HAY6138540.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28304..29677
                     /gene="aroP"
                     /locus_tag="JQH65_001055"
     CDS             28304..29677
                     /gene="aroP"
                     /locus_tag="JQH65_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HAY6138541.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNSGPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29720..30574)
                     /gene="ampE"
                     /locus_tag="JQH65_001056"
     CDS             complement(29720..30574)
                     /gene="ampE"
                     /locus_tag="JQH65_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HAY6138542.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARVTMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGIWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30571..31122)
                     /gene="ampD"
                     /locus_tag="JQH65_001057"
     CDS             complement(30571..31122)
                     /gene="ampD"
                     /locus_tag="JQH65_001057"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HAY6138543.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31210..32103
                     /gene="nadC"
                     /locus_tag="JQH65_001058"
     CDS             31210..32103
                     /gene="nadC"
                     /locus_tag="JQH65_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HAY6138544.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32306..>32489
                     /locus_tag="JQH65_001059"
     CDS             32306..>32489
                     /locus_tag="JQH65_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAY6138545.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHG"
BASE COUNT         7832 a         8833 c         8005 g         7819 t
ORIGIN      
        1 gccacgctac aacaggatgg aactatacca atagaagctg gtgagtttaa agccaccagt
       61 actttccagg taacctaccc ttaataagtc caccgcaccg ccatcatctg gcggtgcggt
      121 aattgataaa tcattgcccc caccaatgtg actgataaaa tgccaggcaa cacgacatca
      181 tttatcagga cacgttatga aaccgaccac cattgcttca cttcagaaat gtaagcagga
      241 taaaaagcgt ttcgcgacca tcaccgctta cgactacagc ttcgcgaaac tatttgctga
      301 tgaaggactt aacgtcatgc tggtaggcga ctcgctgggc atgacggttc agggtcacga
      361 ctccaccctg ccggtgaccg ttgccgatat cgcctaccac actgcctccg tacgtcgtgg
      421 tgcgccaaac tgcctgctgt tggctgacct gccgtttatg gcgtatgcca cgccggaaca
      481 agcctttgaa aacgccgcaa cggttatgcg tgccggtgcc aatatggtca aaattgaggg
      541 cggtgagtgg ctggtcgaaa ccgtaaaaat gctgaccgaa cgtgccgttc ctgtatgtgg
      601 tcacttaggt ttaaccccac agtcagtgaa tattttcggt ggctacaaag ttcaggggcg
      661 cggcgatgaa gcgggcgatc aactgctcag cgatgcatta gccttagaag ccgctggggc
      721 acagctgctg gtgctggaat gcgtgccggt tgaactggca aaacgtatta ccgaagcact
      781 ggcgatcccg gttattggca ttggcgcagg caacgtcact gacgggcaga tcctcgtgat
      841 gcacgacgcc ttcggcatta ccggcggtca cattcctaaa ttcgctaaaa atttcctcgc
      901 cgaaacgggc gacatccgcg cggctgtgcg gcagtatatg gctgaagtgg agtccggcgt
      961 ttatccgggc gaagaacaca gtttccatta aggagtcacg ttgtgttaat tatcgaaacc
     1021 ctgccgctgc tgcgtcagca aattcgccgc ctgcgtatgg aaggcaagcg cgtggcgctg
     1081 gtgcctacta tgggtaatct gcacgatggc catatgaagc tggtcgacga agccaaagcc
     1141 cgcgccgatg tggtcgtcgt cagtattttc gttaacccga tgcagttcga ccgcccggaa
     1201 gatctggcac gttatccacg gaccttgcag gaggactgcg agaagctaaa caaacgtaaa
     1261 gtggatttag ttttcgcccc ttcggtaaaa gagatctacc cgaacggtac tgaaacccat
     1321 acttacgttg acgttcccgg gctttcgacc atgctggaag gcgcgagccg tccggggcat
     1381 tttcgcggcg tttcgactat cgtcagcaag ctgtttaacc tggtccagcc ggacatcgcc
     1441 tgcttcggtg aaaaggactt tcagcaactg gcgctaatcc gcaaaatggt tgccgatatg
     1501 ggcttcgata ttgaaattgt cggcgtcccg attatgcgcg ccaaagatgg ccttgcgctc
     1561 agttcccgta atggttatct gacggcggaa caacgcaaaa ttgcgcccgg tctgtacaaa
     1621 gttttaagtt caattgccga caaattgcag gctggcgagc gggatcttga tgaaattatt
     1681 gccattgcag ggcaagaact gaatgaaaaa ggcttccgcg ccgatgatat tcagattcgc
     1741 gatgccgaca cgttgctgga agtctctgaa accagcaaac gagctgtaat tctggtagcc
     1801 gcctggcttg gcgatgcccg cctgatcgac aacaaaatcg tcgaactggc gtaatgcttt
     1861 tactctaaat aattcaagtt gcaggaaggc ggcaagcgag tgacaaattc gtcaggaacg
     1921 aatttgccca gccgcaggct ggccttcggt gagagacatg gatgtctctc attaatcccc
     1981 aggagcttac ataagtaagt gactggggtt agcgaacgca gacacagcac atgcaattta
     2041 aagtatgacg agtataactg gcgctacggc tgatggcgcc aattattaat ttgccccacg
     2101 tcatggcgat ggcgtctgta agcaattatt cataacgcag acaacttagt taatcagcct
     2161 atcaatctca gcaagagata aatttgtcat ctctgcaacg tcttcccgag acatcccttt
     2221 actcagaaaa cgctgggcga attcttgcct ttcctcctgt cttccctgct gaatcccctt
     2281 ctcaatccct ttctcttcaa accactgcgc cagcgtcatc atagactccc ctcccgtttc
     2341 cctgtctctc agcacaccat aaaacaaatc cgcttgttca gtatgaccgc gttgcagcat
     2401 atagttttgc atggcaacta actgacttcc gctagtgtac ccttcgtcaa tcagcgtgac
     2461 cagttgctcc agcaataaca ttaagtcgcg ctggcgaata tgtttttgca gtagttcgag
     2521 aatcgcaatc cgccgatgtt gcatgatttc gtcatccggt gtgatggtga tatccaccag
     2581 cgggaaagga ctgttataga cgcgtcgtgc cagctccggc gagtaaaata tatcaaacca
     2641 gcacattgag agcggataag gtgtggcctc gccctgataa aacagtatcg gcaccaccag
     2701 cggcagctta tcgtgatccg cctccagatg ccggtgcatg gcagcaatag aataacgcat
     2761 catgcgaaag gccattttct tatccggctt gccttggtgt tcaatcacaa catgcagata
     2821 accgggattg ccctgcattt gcacggaata gagcacgtcc gtgctgtgtc ctttcaggct
     2881 ctcttcaatg aaactccccg actctaaatg aagcgtgttg aggtcacaaa gttctcgtaa
     2941 ttccactggc aaatgtatct ccagaaagtc gcgagccgtc tccgcgtgca ttaaaaattg
     3001 tttaaacact gcatcatgcg gtgtggtact cggtgcatcc atcttccctg ccttccgtgt
     3061 tgtcttgcgt gaaggcagta tccagtcaag gcgtgccatc gtcagccacc tctttagtgt
     3121 ttaccgagca gcgttcagag aaatttcaat tggttacagc cgttacatgg cacgcttcgg
     3181 cacggtacgc aaactgctga tgtaaatgcc gtcagatgag tagacactaa acaaaaatcg
     3241 ggcaatactg cgtgagaatt tcctcaaagc aggcatcgcc tgcttcgtta acgacagggt
     3301 agaaaggtag aagttatgat tcgcacgatg ctgcagggca aactccaccg cgtgaaagtg
     3361 actcatgcgg acctgcacta tgaaggttct tgcgccattg accaggattt tcttgacgca
     3421 gccggtattc tcgaaaacga agccattgat atctggaatg tcaccaacgg caagcgtttc
     3481 tccacttatg ccatcgcggc agaacgcggt tcgagaatta tttctgttaa cggtgcggcg
     3541 gcccactgcg ccagtgtcgg cgatattgtc atcatcgcca gcttcgttac catgccagat
     3601 gaagaagctc gcacctggcg acccaacgtc gcctattttg aaggcgacaa tgaaatgaaa
     3661 cgtaccgcga aagcgattcc ggtacaggtt gcttgattgt tatccctgcg gctggttact
     3721 caccagccgc gacatcgtct ccagcgaatc cgttcttaag atataaagtc gttttagtaa
     3781 caacggatta tcccccggtt ttactttgcc tttcatggtt gtcaccgcca ggtgaaatcc
     3841 ggcatcgttt gctgccttca cggctttgtc attaaatccg ccaaacggat acgaaagata
     3901 ccagacatgc ggattaaatt gcgccagagc gcggcgtgaa cgtgcaaaat caaacagaat
     3961 attgtgttca ctacggctcg gtaatatggg gcggcgataa ccatccaccc gatgcaaaaa
     4021 atgggtatgt gactggaaat caaaaacatc gcgaatttcg ttaagttcag aaacgctcat
     4081 aaattgcagc gattttgggt cccacttctg cgggtgacgt ttgatgcgcg aggtaacaat
     4141 aaacgccgtc gccttcatgc catattgttt caacacggga tacgcatagc ggctcaccga
     4201 tttgaggcca tcatcaaagg taatcaccac cgctcgcgca gggagattga tcttattctt
     4261 cacgtaacct tccagctgca ccatgctcag tgtcgcgtat cccctgtcgc gcagccaggc
     4321 catctggtta ttgaaagcgc gtactgaggt ggtcgtcgaa gtatggcgaa aacgggtgtt
     4381 ttcttcatcg cgcagaatat ggtgataggt tagcaccggc aggccattat cgggttgggc
     4441 atccagcgcg ctgatatagg ccagtcgatc gccaatacgg atctgatacc aggtttgatt
     4501 taacctgtct ttcagtttat gcaaaatcgg gtagcgcaaa ttgtccgcca gtaccccata
     4561 atggcgcact gcccgcactc ggcgcgttat agaccggcgt atctttccag gtaattaagt
     4621 tctgattact cagcggcttg ttgaggtcac ccaaaccgtc ttcaactttt tgtcgcccct
     4681 gaactggctc gagatgccct ttatcgataa accctttacc aaagccaaaa ttaaatgcgt
     4741 aataacttgc ggcagtgggc tccacggcaa tgatttgccc ggcccgaata ttccccacgg
     4801 tcaccatctt gtcgccaatt tgcgcccaga tcgcagcatt ttcgatggtt tgcatataac
     4861 gggcaggtaa cgcggcacta acgcttgcgg taaacagcat cagcaggatg aataacagct
     4921 tgtttataca tgaaaaaata accgagggtg aaacaacgct tgtattgtag caaaagcgcc
     4981 atcagaatcg cgggttaata ctcatacaaa ttatgtttga ggacaaaagg cggatttttt
     5041 tgttgttggt gccaaagact actcacctcc ggcgcaccca gttcgacaat acgctcgcga
     5101 aactctgaac tggtcatggt ttcacggcaa gccatcatct gttcaattaa cggtaacgtg
     5161 acaccagaaa tcacttcaca acgggagtgt ttatggctta ataatgaagc cacgcggtac
     5221 ggtggcgctc cggctatatc ggttaagaag atgacaccct caccagagtc cgcttcatgc
     5281 agagcatcgc acatcatgcg gctgagcata ttagagctta atccgcgcca gaaattcacg
     5341 gcccggcact gaagaagtgc cccatgtttt ttctccagcg catccagtat ctcttgcgcc
     5401 cggtcatcgt gacaggtaat tacccaacct aacatcgcct tcctccttag ccgaaaatca
     5461 gtctatcctg accattgttg ataaagggtg atagccgtca aaactcctct cccccgaatc
     5521 cagaggagag gaaatagcct tagctacgca aaccacgtcc acgttggatc agcgaccaac
     5581 agatcaaata aaacaccaca ataaagacca ccagtacgcc aaaggtagtg gccagcggca
     5641 catcatttat accgaggaag ccgtagcgga aaccactgat catataaacg attgggttca
     5701 ggtgcgacag cccttgccag aacggcggca acaaagtcag ggagtaaaac accccgccca
     5761 aatacgtgag tggcgttaac acaaaggttg gcaccaggct gatgtcatcg aacgttttgg
     5821 caaacacgcc gttcagcaaa cccgcaaggg agaatagcac cgccgtgagc accagggtta
     5881 aggcaacgaa tacccacgaa tgcacctgaa acggcacaaa aaacagtgaa attgccgtca
     5941 ccagaatgcc aacaaacaga ccacgcgcca cgccaccgcc gacatatccg gcaataatga
     6001 cgtgagtcgg aaccggcgct accagcagct cttcaatatt acgctggaac ttggcaccaa
     6061 aaaatgatga cgcgacgtta gcgtaggcat tggtgatcac cgacatcatg atcagccccg
     6121 gtacgatgaa ctgcatatag ctgaagccat gcatatcgcc aatacgcgaa cctatcaggt
     6181 taccgaagat aataaagtaa agggtcatgg tgatgactgg cggcaccagc gtctgcaccc
     6241 agatacgcat aaagcgatgg atctctttcg cccagatgct ttttagcgcc acccagtaaa
     6301 gatgcatcat gcgcgatctc cttgtttttc attaaccagt gaaacaaaca gctcttccag
     6361 acggttagct ttgttacgca tacttaatac ctgaatgccc tgctcactta actgcgtaaa
     6421 tacgctgttg atcccctgct cacgcagcac ttcaacttcc agcgtcgcgg tatcgaccag
     6481 tcgatactga tagccatcga gcttcggtaa cgggcttttc ggtgcgagat cgagaataaa
     6541 ggtttccaat ttcagcttcg ccagcagcgc cttcatcgag gtattttcca ccagctcacc
     6601 gtgttgaata atgccgatat tgcggcacag catttctgct tcttccaggt agtgtgtagt
     6661 gagaatgatg gtggtgcctt tgtcgtttaa atccttcaaa aagccccaca ttgagcggcg
     6721 aagttcaata tccacgcctg cggtcggttc gtcgagaatc agtagtttag gttcatgcat
     6781 taacgcacgg gcaatcatta aacggcgctt catcccgcca gataacatac gcgcacgttc
     6841 gttgcgtttt ccccataaat cgagttgttt gagatatttt tcgctgcgga tgtacgcttc
     6901 tttgcgctcc acgccgtagt accctgcctg attcaccaca atttgctgca cggtttcaaa
     6961 cgggttgaag ttaaattcct gcggcaccag tcccaactga cgtttagcgt tcacgacatc
     7021 cttctcgaga tcgtaaccaa atacgctgac ccgcccggag gttttattta ccagagagct
     7081 gataataccg atagtggtcg atttcccggc cccgttcggc ccgagaagcg cataaaaatc
     7141 acccgcttcg acctgcaaat ctattccacg aagcgcctga acgccgcctg gataggtttt
     7201 tttaagctgt tgaagttcca gtgcaatggt cataaatttt tacttacctt acgttcttac
     7261 actttatctg tggtttaaat cgtcccggag ttaccctata ttagccaaac gtaattattt
     7321 ggttacaggt cgttaacctc catgaaagac atagatacac tcatcagcaa caatgcacta
     7381 tggtcaaaaa tgctggtgga agaggatccc gggttttttg agaaactggc acaagcgcaa
     7441 aaaccgcact ttctatggat tggatgttcc gacagtcgcg ttcctgcaga acgtttaacc
     7501 ggtcttgagc cgggcgaact ctttgttcac cgtaatgttg ctaacctggt cattcacacc
     7561 gacctgaact gcctttccgt ggttcagtat gcagtggatg tactagaagt tgaacacatt
     7621 attatctgtg gccactacgg ttgcggcggc gtacaagccg cagttgaaaa cccggaactg
     7681 gggcttatca acaactggct gctgcatatc cgcgatatct ggttcaaaca tagctcattg
     7741 ctcggcgaaa tgccgcaaga gcgccgtctg gataccttgt gtgaactgaa cgtcatggaa
     7801 caggtgtata acctaggcca ctccaccatt atgcaatcag cgtggaaacg cgggcagaaa
     7861 gttaccattc acggctgggc ctatggcatt cacgacggct tgctgcgtga tctggatgtt
     7921 actgccacca accgcgaaac ccttgagcaa cgttaccgtc acgggatttc caacctcaag
     7981 ctgaaacacg ccaaccacaa ataaaaatgc catgccggat gcaacacatc cggcaacttc
     8041 acacttactc gtccagcaga atcactttgc cgatatacgg cagatgacgg taacgctgtg
     8101 cgtaatcaat gccgtaaccc accacaaact catccgggat cgagaaaccg ataaattcta
     8161 ccgggacgtt cacttcacga cgggacggtt tatccagcag cgtacaaatc gccagcgact
     8221 tcggttcgcg caggcttaag atctcacgca ctttcgacag tgtattcccc gagtcgatga
     8281 tatcttcaac aatcagcacg tccttgccac ggatatcttc atccagatct ttgaggattt
     8341 tcacatcacg ggtggtggac atgccgctac cgtagctgga ggcggtcata aagtcgactt
     8401 catgagatac ctgaacttca cggcacaggt ccgccataaa cataaatgag ccacgcagca
     8461 gacccaccag caccatatcg ctgccgctgt ctttgtaacg ctcagtaatc tgacgaccca
     8521 gttcggcgat acgcgcttta atctccgctt cggggatcat tacttctaca gtatgtttca
     8581 tatctctaac catatgattt aaaaacaaat cacttcgcac gggcaggttt tggcaccgca
     8641 acaaagcgca agacacgcag tataccagta aaactattca ttcgcggaat ctgttaataa
     8701 agctcgaatt gtgatgacga tcacacatgt taaaacctat aattaattgc tactaaaata
     8761 ttaatgaatt gaaatggtgt ctctttatgg caattaacaa tacaggctcg cgacgattac
     8821 tcgtcacgct aacagtcctt tttgcagcgc tttgcgggct gtatctactc attggcggag
     8881 gctggctggt cgcgattggc ggctcctggt actaccctat cgctggcctt gtgatgctcg
     8941 gcgtcgcctg gatgctgtgg cgcagtaaac gtgccgcgct ttggctatac gccgccctgc
     9001 tgctcggcac catgatttgg ggcgtctggg aagttggttt cgacttctgg gcgctgactc
     9061 cgcgcagcga tattctggtc ttcttcggca tctggctgat cctgccgttt gtctggcgtc
     9121 gcctggtcat tcctgccagc ggcgcagttg ccgcactggt ggtggcactg ctgattagcg
     9181 gtagtatcct gacctgggcc ggatttaacg atccgcagga aatcaacggc accttaagcg
     9241 ccgatgccac acctacggaa gctatctcac ccgttgccga tcaggactgg cctgcctatg
     9301 gtcgcaatca ggaaggtcaa cgcttttcac ctctgaaaca aattaacgcc gataacgtcc
     9361 ataatctgaa agaagcctgg gtgttccgta ctggcgatgt gaagcagccg aacgatccgg
     9421 gcgaaatcac caatgaagtg acgccgatta aagtgggcga caccctttac ctgtgtaccg
     9481 ctcaccagcg cctgtttgcg ctcgatgccg ccagcggcaa agagaaatgg cattacgatc
     9541 ctaagctgaa aactaacgag tctttccagc acgtaacctg ccgtggcgtc tcttatcatg
     9601 aagccaaagc agaaacggct tcgccggaag tgatggcgga ttgcccgcgt cgtatcattc
     9661 ttccggtcaa tgatggtcga ctgattgcga ttaacgctga aaacggcaaa ctgtgcgaaa
     9721 ccttcgccaa taaaggcgtg ctcaatctgc aaagcaatat gccagacacc aaaccgggtc
     9781 tgtatgaacc gacttcgcca ccgattatca ccgataaaac catcgtgatg gcaggttcag
     9841 ttaccgataa cttctcaacc cgcgaaacgt ctggcgtgat ccgtggtttt gatgtcaaca
     9901 ccggggagct gctgtgggct tttgatcccg gcgcgaaaga tccgaacgca atcccgtctg
     9961 acgaacacac ctttaccttt aactcgccaa actcctgggc accagcggcc tatgacgcga
    10021 agctggatct ggtctatctg ccgatgggcg tgaccacgcc ggatatctgg ggcggtaacc
    10081 gcacaccgga acaggaacgt tatgccagct cgattctggc gctgaatgcc actaccggga
    10141 aactggcgtg gagctaccag accgttcacc acgacctgtg ggacatggat cttccggcac
    10201 agccgacgct ggcggacatc accgttaatg gtcagaaagt gccagttatt tacgctccgg
    10261 cgaaaaccgg caacattttt gtgctcgatc gtcgcaatgg cgaactggtg gttccggccc
    10321 cggaaaaacc ggtgccgcaa ggtgctgcga aaggcgatta cgttactccg acccagccgt
    10381 tctctgaact gagcttccgt ccgaagaaag atttgagcgg tgcggatatg tggggagcca
    10441 ccatgtttga ccaactggtg tgccgcgtga tgttccatca gatgcgctat gaaggcattt
    10501 tcacgccgcc atctgaacag ggcacgctgg tcttcccggg taacctggga atgtttgaat
    10561 ggggcgggat ttccgttgat ccaaatcgtg aagtggcaat tgccaaccca atggcactgc
    10621 cgtttgtttc gaaactgatc ccacgcggtc cgggtaaccc gatggagcag ccgaaagatg
    10681 ccaaaggcac gggtacggaa tccggcattc agccacagta cggtgtaccg tatggtgtca
    10741 cgctcaaccc gttcctctca ccgtttggtc tgccgtgtaa acagccagca tggggttata
    10801 tctcggcgct ggatctgaaa accaatgaag tggtgtggaa gaaacgtatt ggtacgccgc
    10861 aggacagtat gccgttcccg atgcctgtac cggtgccgtt caatatgggt atgccgatgc
    10921 tgggcgggcc aatctccacg gcgggtaacg tgctgtttat cgccgctacg gcagataact
    10981 acctgcgcgc ttacaacatg agcaacggtg aaaaactgtg gcagggtcgt ttaccagcgg
    11041 gtggtcaggc gacgccaatg acctatgaag tgaatggtaa gcagtatgtg gtgatctccg
    11101 caggcggtca cggttcattt ggtacgaaga tgggcgacta tattgtggct tatgccctgc
    11161 cggatgatgt gaaataacca taatgcccgg agagatccgg gcatatttcc gaatacggtc
    11221 tttttatacc gtaaacccta acatcatccc cgtatcttca tgctccagca gatggcagtg
    11281 cgccatataa gcatgttctt tcggtgcgtc gtgattaaac ttcaccagca cttcactgac
    11341 attgccttct accttaacgg tatctttcca gcccgcgcga tgagccgctg gcggtttgcc
    11401 attttctgac aagatacgga actgcgtgcc gtgaatatgg aacggatgca gcatcatgtc
    11461 gcccacgcca gagataaccc aacgttcgta ctgccctttc gccgccgcaa acatcggctt
    11521 attcatatca aacgcctgac cgttgatttt attggcatgg tggaaatcga acttcccgcc
    11581 gtggttcata tgattcatat taccgtgccc catatggccc atcatctggc tgtgatccat
    11641 tccggccatc gcctgatcgc catatttctc cattagcatc tgcataccca tcatatcgag
    11701 catcgggtcc atagaaagtt gcagcttgcg taccgtcagc ccttccagcg aaggtaacgc
    11761 aggcaggcta cttaatgtgt ctggcaaagc accggaggca ctaatagcaa tcggctgaat
    11821 ccgcattacc ggatgcggct tgtcaaacgg cgcaatcgcc atccccatct ggctgaccgg
    11881 tagcgtcacc aggtcaaagg gtttgttgtc gttaacctcc accagcactt caaaacgctc
    11941 gcccatcagc accggcagct cgctcacctt caccggttca ggtagcagac caccgtcgct
    12001 ggcaatcaca tacagcgggc gattgtcgct ggtggcgaaa ttgagcgagc gggcattaca
    12061 gccattgagc aaacgcaggc gcagccaacc acgcggggca gcgtgttgtg ggtagattgc
    12121 accgttggtc agcagcgtat cgccaaacca gcccacggcg gcggtcatca catccagttg
    12181 ataatcaatc tgcccatcag cgttaaattt tttatcctga acaatcaccg gaacatcatc
    12241 gataccccac tgttttggca gcattaattt caggatctcg tcatcttcaa ttaccaccag
    12301 cccagccaac cccatcgcca cctgtcgccc agtcttgcca tgttgatgtg gatggaacca
    12361 gcaagtagcg gcaggttgat caacgttcaa cgtcaccgag cgcttgccac ctggcggaat
    12421 aattccctgc gggccgccgt cgacttcacc cggtacttcc agcccgtgcc agtgcaacgt
    12481 cgtctcttcc gtcagttggt tatagatatc aaccgttacc gctttgccgc actgtaattt
    12541 caccgccggc cccagcagat tgccgttata gccccaggta gttgcagttt tctcgccaaa
    12601 ggtagactga cctgcgccaa tagttaactg aatgcgatta cgggcatcgg tcgtgagcaa
    12661 atcagggatc ggtaacgttg ggcgttccgc cgcaaagact gcgcggctcc acagcggcaa
    12721 ggctgaagcc acacccagcg cgacggaata ttttaagaaa tcacgacgtt gcatagttat
    12781 ttccttattc ttaagcaggc gaaacaaaat caaacgttga gcatagtcct tccccttacg
    12841 ggaaggtcaa gccgcagaca caatttaatc gacaataatg gtcgtcgcct cgctcgcagt
    12901 gtgctaacgt ttatcttctt taagcccctg gtagaagcaa tgaagacgtt tttcagaaca
    12961 gtgttattcg gcagcctgat ggccgtttgc gcaaacagtt acgcgctcag cgagtctgaa
    13021 gccgaagata tggccgattt aacggcagtt tttgtctttc tgaagaacga ttgtggttac
    13081 cagaacttac ctaacgggca aattcgtcgc gcactggtct ttttcgccca gcaaaaccag
    13141 tgggatctca gtaattacga caccttcgac atgaaagccc tcggtgaaga cagctaccgc
    13201 gatctcagcg gcattggcat tcccgtcgct aaaaagtgca aagccttggc ccgcgattcc
    13261 ttaagcctgc ttgcctacgt caaataatcc ctgatacctt tttgtagaaa tattgaccgt
    13321 gcatccgcgg tcaatgttag ctattatgtt gcgccctttt tttacgggtg ttaacaaagg
    13381 aggtatcaac ccatggccga aaaaaaacag tggcatgaaa cgctacacga ccagtttggg
    13441 cagtactttg cggtagataa cgttctgtat catgaaaaga ccgatcacca agatctgatc
    13501 atttttgaga acgctgcatt tggtcgcgta atggcgctgg atggcgtagt acaaaccacc
    13561 gagcgcgacg agtttatcta tcatgagatg atgacccatg ttccgctact ggcccacggt
    13621 cacgcgaaac atgtgctgat tatcggcggc ggcgacggtg ccatgctgcg tgaagtaacc
    13681 cgacataaaa acgttgagtc aatcacgatg gtagaaatcg atgcgggtgt cgtgtcgttc
    13741 tgccgtcagt atctgcccaa ccataacgcc ggtagctacg acgatccgcg ctttaagctg
    13801 gtgatcgacg atggcgtcaa tttcgttaat caaaccagcc agacctttga tgtcattatc
    13861 tccgactgca ccgatcctat cggtcccggc gaaagtctgt tcacctcagc attttatgaa
    13921 ggctgcaaac gttgcctgaa tcctagcggt atcttcgtcg cacaaaacgg cgtctgcttt
    13981 ttacagcagg aagaagccat cgacagccat cgcaaactca gccattactt cagcgacgtt
    14041 ggcttttatc aggcggcgat cccgacctat tacggcggta tcatgacttt tgcatgggcg
    14101 acagataacg acgccttacg ccatctctca accgaaatta ttcaggcgcg ttttctcgct
    14161 tctggcctga aatgccgtta ttacaatccg gcaatccata cggcagcttt tgccttacct
    14221 cagtatctgc aagatgcact ggcttcacag ccgtcctaag gagaagataa gaaattgaaa
    14281 aaactgaaac tgcatggctt taataatctg accaaaagtc tgagtttttg tatttacgat
    14341 atctgctacg ccaaaactgc cgaagagcgc gacggttata ttgcttatat cgatgaactc
    14401 tataatgcca accgtctgac cgaaatcctg tcagaaacct gttccattat cggggccaat
    14461 attcttaaca tcgcccgcca ggattacgaa ccacagggtg ccagcgtcac tattctggtg
    14521 agtgaagaac cggttgaccc gaaactcatc gacaaaacag aacaccccgg cccactgcca
    14581 gaaacggtcg ttgcccatct cgataaaagt catatttgcg tacataccta cccggaaagc
    14641 catcctgaag gtggtttatg taccttccgc gccgatattg aagtctctac ctgcggcgtg
    14701 atttctccgc tgaaggcgct gaattacctg atccaccagc ttgagtccga tatcgtaacc
    14761 attgattatc gcgtgcgcgg ttttacccgc gacattaacg gtatgaagca ctttatcgac
    14821 catgagatta attcgattca gaactttatg tctgacgata tgaaggcgct gtatgacatg
    14881 gtggatgtaa acgtctatca ggaaaatatc ttccatacca agatgttgct taaagagttc
    14941 gaccttaagc actacatgtt ccacaccaaa ccggaagatt taaccgacag cgagcgccag
    15001 gaaattaccg ctgcgctgtg gaaagaaatg cgcgagattt attacgggcg caatatgcca
    15061 gctgtttaac ggctctggcg gagctccagg ctccgccaga tttatttact tctgctgcac
    15121 gaaattgcgg taagccgcca cgacctgcag aaaatcctca acgccgcata gcgacaggct
    15181 ttcttcgtcg tagtagttca tcccctcttc catttcatcg ccagcgaatt ccagttgatt
    15241 ggcgcgaacc atcacctctt caccgtccat ccacagggtg tattcatgcc ctgcccgttg
    15301 ccaggaccgt tcgctacctt tcagtgcgtg cgctgcttgt tccacttcat caagcaaggc
    15361 caggttttct ttcacctctt cattaaacca gtgcccgacc acttcatgcc ccatggacat
    15421 acgcaccttt accactccgg taatatcgcg cagaaattcg taatccatag tgtgttcctc
    15481 tgctcccggc aatgcgccga aatcgcattg cgccattgat gtaattatcg cagccttacc
    15541 ggagaaaaac agcggaacgg cgatggttta gaaataaaaa atgcccggga atctcattca
    15601 ccgggcattg tgtcgtttat gcgcagcgcg tgcgcttact tattacaccg cagtctggaa
    15661 aatcacccca tcggcttttt cggtgtactg agaaagttgg ttgaagttca gataacgata
    15721 agtatcaacg gcagttttat ctacttgcgc aacgtaggtc tggtactctt ccggcgtcgg
    15781 cagtttgcca atcagcgccg caacagccgc cagttctgca gaagccagga agacattcgc
    15841 gccagtcccc agacggttcg ggaagttacg ggtagaggtg gaaaccaccg tcgcaccgtc
    15901 tgctacacgc gcctggttac ccatacacag agaacagccc gggatctcga tacgagcccc
    15961 gctcttaccg aagacgctgt agtagccttc ttcagtcaac tgtgcggcgt ccatacgggt
    16021 tggcggtgcc acccacaggc gggtcggcaa ctggccttta tgcgcatcca gcagtttacc
    16081 cgcagcacgg aagtgaccga tgttagtcat gcaagaaccg ataaatactt cgtcgatctt
    16141 ctcgccctgt accgcagaca gcggacgcgc gtcatccggg tcgttcggag cacacaggat
    16201 tggctcttta atatccgcca gatcgatgtc aatcactgcc gcgtattccg catctgcatc
    16261 tgcttccagc agctcaggat tcgccagcca tttttccatg ccctgaatac gacgttccag
    16321 ggtacgacga tcgccgtaac cttccgcgat catccacttc agcaggacga tattagagtt
    16381 aaggtattcg atgattggtt ctttgttcag cttgatagta caaccagcgg cagaacgctc
    16441 agcggacgca tcggtaagct caaaggcctg ctcaactttc agatccggca gaccttcaat
    16501 ttccagaatg cggccagaga agatgttttt cttgcctttc ttctcaacgg tcagcagacc
    16561 ttgtttgatg gcatacagcg gaatggcgtg taccagatcg cgcagggtga tgcccggctg
    16621 cattttgcct ttgaagcgca ccagaacgga ttccggcata tccagcggca ttacgcctgt
    16681 tgcggctgca aatgccacca gaccagaacc cgccgggaaa gagataccga tcgggaaacg
    16741 ggtatgggag tcacctccgg taccgacggt atccggcagc agcatacggt tcagccagga
    16801 gtggattacg ccgtcacccg gacgcagcga cacaccgcca cggttcataa tgaagtccgg
    16861 cagcgtgtgg tgcgtgttca cgtcaactgg cttcggatac gccgcggtgt ggcagaaaga
    16921 ctgcatcacc aggtcagccg agaagcccag gcacgccagg tctttcagtt catcacgggt
    16981 catcgggccg gtagtgtcct gtgaacctac agaagtcatt ttcggttcgc agtacgcgcc
    17041 cggacgaatg cctttcacgc cacaggcacg gcctaccatt ttctgcgcca gcgagaagcc
    17101 gcgatcgctc tcagcgacat ctttcgcctg acggaacaca tcactgtgcg gcagaccaag
    17161 tgcttcacgc gctttggtgg tcaggccacg cccgataatc agcgggatac ggccgccagc
    17221 acgcacttca tcaatcagca cgtcggtttt cagttcgaag gtcgccagca gttcgcctgt
    17281 ttcgtggtta cgcacttcac ctttgtacgg gtaaacgtca atcacgtcgc ccatgttcag
    17341 gttagagacg tcgacttcga ttggcagtgc acccgcgtct tccatcgtgt taaagaagat
    17401 gggtgcaatt ttaccgccga ggcacaaacc accgccgcgt ttgttcggca catgtggaat
    17461 atcatcgccc ataaaccaca gaacggagtt cgtggcggat ttacgcgatg aacccgtacc
    17521 cacaacgtca ccgacgtacg ccagcgggaa acctttctgt tgcagagctt caatctgttt
    17581 gatcgggcca acaacacctg gctggtctgg ctcaatacct tcacgggcgt ttttcagcat
    17641 cgccagcgcg tgcagtggga tatccgggcg cgaccacgca tccggtgccg gagaaaggtc
    17701 atcggtgttg gtttcgccag tgactttgaa gacggtaacg gtcagttttt cagccagcgc
    17761 cgggcgattc agaaaccatt cggcatccgc ccaggactgc attacctgct tcgcatattc
    17821 gttgcctgct ttcgctttct cttctacgtc atagaagtta tcgaacatca gcagtgtgtg
    17881 agaaagtgct ttggcagcga tcggtgccag tttggcatca tccagcgcgt cgatcagcgg
    17941 atgaatgttg taaccaccct gcatggtgcc cagcagttcg atggcttttt ccggagtcag
    18001 cagaggggat ttggcttcgc ctttcgcgat agcagccagg aagcctgctt tgacataggc
    18061 ggcttcatcg acgccagggg gaacacggtt ggttaacaga tctaacagga attcttcttc
    18121 gcccgcgggc gggtttttca gcagctctac aagtgcggcc atttggtttg catccagggg
    18181 tttgggcgca atcccctcag cggcacgctc agctacgtgc ttacggtatt cttctagcac
    18241 gacggttctc ctcgctctca ttgtcatagt gcggcaggcg attctcttca cgctcctgtg
    18301 agacagcagt ttgtagggta aatgcccagt accgcaacgg gcagaatagc aggattttga
    18361 cgacgtgtta atctgtttac aaaaaagcaa cataaaaaag tggctgaatc gttaaggatg
    18421 gtctagagat tgtttccctc cgtaaaaact tcgttaattc gcatggtgat aatcaccttt
    18481 ctcaacgaat cccaagatta gtcaaaattt aaactaccgc ctctttatac tcggattaac
    18541 agcacctgcg ggtggcagtt cgccgaccat tgcgatttcc ttgagatccg aattatgaaa
    18601 atgactttgc cgtttaaacc ccatgtgctg gcactaattt gcagtgccgg gctttgtgcc
    18661 gcctctgccg ggctatatat aaaaagccgc acagtggaag cggctgtgga aacgcaatcg
    18721 acacaactgg ctgtgtctga cgctgccgcg gttacgcttc ctgcaacggt ttccgcgcct
    18781 cccgtaacac ccgccgtcgt taaatccgca ttcagcactg cacaaataga tcaatgggtc
    18841 gcgcccgtcg cgctgtatcc cgacgcccta ctttcgcagg tgctgatggc atcaacctat
    18901 ccggcaaacg ttgctcaagc agtgcaatgg tcgcacgata atccacttaa acaaggcgat
    18961 gctgctattc aggcggtatc tgaccagccg tgggacgcca gcgttaaatc actggtggcc
    19021 tttccacaat tgatggcatt gatgggcgaa aacccgcaat gggtgcaaaa cctgggcgat
    19081 gcttttctgg cgcagccgca ggacgtgatg gactcggtac agcgattgcg gcaactggcg
    19141 caacaaaccg gttcgctgaa gtcatcaaac gaacaaaaag ttattaccac aacgaagaaa
    19201 gctgtaccgg taaaacagac agtcacggca cccgtcatac catccaatac cgttttaact
    19261 gccagccccg tcattacaga gcctgcaaca accgtcattt ccattgagcc cgccaatcct
    19321 gatgtggtct atattcccaa ctacaaccca accgtggttt acgggaactg ggccaatact
    19381 gcgtatccgc cggtttatct gccaccacca gccggagaac cgtttattga cagctttgtg
    19441 cgcggattcg gctatagcat gggtgttgct accacgtacg cactattcag cagcatcgac
    19501 tgggatgacg acgatcatga ccatcatcat catgacgatg atgattatca tcaccacgat
    19561 ggcggtcatc gtgacggtaa tggctggcaa cacaacggcg acaacatcaa tatcgacgtc
    19621 aacaatttca accgtatcac cggtgagcat cttactgata agaatatggc atggcggcac
    19681 aatccaaact accgtaatgg tgtgccctat catgatcagg atatggcaaa gcggtttcat
    19741 caaaccgatg tcaacggcgg aatgagtgcc acgcagctac ctgctccaac acgcgacagc
    19801 cagcgtcagg cggcagcaag tcagtttcag caacgaacac acgccgcccc cgtcattaca
    19861 cgagataccc aacgtcaggc agcggcacag cggtttaatg aagctgaaca ctatgggagc
    19921 tatgacgact tccgcgactt cagccgtcgc caacccctga cccagcaaca aaaggacgcc
    19981 gctcgtcagc gttatcagtc agcttctcct gagcagcgcc aggcagttcg cgagagaatg
    20041 cagactaacc cgcagatcca gcagcgaaga gaggcagcgc gtgagcgcat tcagcccgcc
    20101 tcgcctgagc agcgccaggc agtccgcgag aaaatgcaga ctaacccaca gatccagcag
    20161 cgaagagacg cagcgcgtga gcgtattcag tcagcctcgc ctgagcagcg ccaggtgttt
    20221 aaggaaaaag tacagcagcg cccactgaac caacagcaac gtgataacgc ccgccagcgt
    20281 gttcaatcag catcacctga acaacgtcag gtttttcggg agagagttca ggagagccgc
    20341 ccacaacgtc taaacgacag taaccgtact gccagattga ataacgatca acggtcagca
    20401 gtacgcgaac gtctctctga gcgcggagca aggcgactgg aaaggtaaat tacagacgta
    20461 aaaaaagcgg cgtggttagc cgctttttta attgccggat gttccggcaa acgaaaaatt
    20521 acttcttctt cgctttcggg ttcggcaggt cggtaatgct accttcgaac acttctgccg
    20581 ccaggcccac agactcgtgc agagtcgggt gcgcatggat ggtcagcgcg atgtcttcag
    20641 cgtcacaacc catttcgatt gccaggccga tttcacccag cagctcgccg ccgttggtac
    20701 cgacaatcgc accaccgatc acacggtgag attctttgtc gaaaatcagc ttggtcatac
    20761 cgtctgcgca gtcggaagcg atagcacgac cagaagcagc ccacgggaag gtggcggttt
    20821 catagctgat gcctttctct ttcgcttctt tctcagtcag acctacccat gcaacttctg
    20881 gttcggtata ggcgatagac gggataactt tcggatcgaa gtagtgtttt ttaccggcga
    20941 taacttcagc ggcaacgtga ccttcgtgaa cacctttgtg tgccagcatc ggctgaccaa
    21001 cgatatcgcc gatagcaaag atgtgcggta cgttggtacg cagctgtttg tcaacgcgga
    21061 tgaaaccacg gtcgtccact tccacgccag ctttgcctgc gtcgaggttt ttaccgttcg
    21121 gcacacgacc aatcgctacc agcacggcgt cgtaacgctg cggttcagcg ggtgcttttt
    21181 tgccttccat cgtcacataa ataccgtctt ctttcgcttc aacggcggta actttggttt
    21241 ccagcatcag gttgaatttc ttgctgatac gcttggtgaa gactttaacg atgtctttgt
    21301 cagctgccgg gataacctgg tcgaacattt caaccacgtc aatctgtgaa cccagcgcgt
    21361 ggtatacggt gcccatttcc agaccgatga taccgccacc cattaccagc aggcgttctg
    21421 gtacttcttt cagttccagc gcgtcagtgg agtcccagat acgcggatct tcatgcggaa
    21481 taaacggcag ttggatcggg cgagaacccg ctgcaatgat cgcgttgtcg aagttgatca
    21541 cggttttacc gttctcacct tcaacttcca gggtgttagc cccggtaaat ttacccagac
    21601 cgttgaccac tttgactttg cggcctttcg ccataccagc cagaccaccg gtcagctgat
    21661 tgatcacttt ctctttccag gtacgaatct tgtcaatatc ggttttcggt tcgccgaaga
    21721 cgataccgtg ttcagccagc gctttggctt cttcgataac ttttgctacg tgcagcagtg
    21781 ctttagaagg gatacagccg acgttcaggc aaacaccgcc aagggtgttg taacgttcta
    21841 cgattacggt ttccagacct aaatcagcgc aacggaaggc agcggagtaa cctgcggggc
    21901 ctgccccaag taccacgacc tgagttttga tttcagtact catcatgacc tctatatatt
    21961 tatctccggc ggtcataccc gtcgtctttc aggccgcagc ggcgttagct tcacgccctc
    22021 accccagtca cttacttatg taagcccctg gggattctgg cgcttgctac cttgctgcac
    22081 ccagaaatcc atagggtatg tgaagtaata ccctaaccac caccgggtcg ttctatccgt
    22141 cggtatttta caaaattgtt aacaattttt aaacaacaaa cggcaaccga tttgtctatt
    22201 cgctaataac ctcaatacat tcatgagatt accagaaaaa agccggccgt tgggccggct
    22261 cttttactta catcaccaga cggcgaatgt cagacagcgt gttgttaatg atggtaatga
    22321 aacgggcacc atcagcaccg tcgatcacgc ggtggtcgaa ggagagagaa atcggcagca
    22381 tcagacgcgg cacgaactct ttaccattcc acaccggctc catcgcggac ttggaaacgc
    22441 cgaggatagc cacttccggc gcgttcacaa tcggcgcgaa gtgggtagta cccaggccgc
    22501 cgatgctgga gatggtgaag caaccgccct gcatttcgcc cgcagtcagc ttaccgtcac
    22561 gcgctttctt agaaatagtc atcagctcgc gagacagctc gatgatgcct ttcttgttga
    22621 cgtctttgaa taccggaaca accagaccgt tcggggtatc caccgccaca ccgatgttga
    22681 tgtatttctt cagggtcaga cgctgaccgt cttccgacag cgaactattg aagcgaggca
    22741 tctgctcaag agctgcagca acggctttca tgatgaagac aaccggggtg atcttcacat
    22801 ccagcttacg tttcgccgct tcttcgttct gctgtttacg gaacgcttcc agctcggtga
    22861 tatcggtttt gtcgaagtgg gtaacatgcg ggatcatcac ccagttacgg ctcaggttag
    22921 caccagaaat tttctggata cggcccagtt ccacttcttc gatttcacca aacttgctga
    22981 agtccacctt cggccacggc agcatgcctg ggataccgcc gccagtcgcc gccggagctg
    23041 cttctgcacg tttgatagct tctttcacgt aagcctgaac gtcttcgcgc aggatacgac
    23101 ctttacggcc agtgcccttc actttcgcca ggttaacacc aaactcgcgt gccagacggc
    23161 ggatcagcgg agtcgcgtga acataagcgt cgttttcagc aaattcagat ttgccttccg
    23221 ctttcgcagc tggtgctgct gccggggctt cagcttttgc tgccggtgcc ggcgctgccg
    23281 cttcctgttt cgcaggagct gccgcaggcg ctgcgccttc aacttcgaag atcataatca
    23341 gcgagccagt tttcacttta tcgccaacgt tgactttcag ttccttcacg acgcctgcaa
    23401 acggcgccgg aacttccata gaagctttgt cgccttctac ggtgatcagt gactgttcag
    23461 cggcaacttt gtcgcccact ttcaccatca cttcagtcac ttcaacttcg tcaccgccga
    23521 tatccggaac gttaacttct ttcacgccag ctgctggtgc aggggccgct gccggagccg
    23581 cttcctgttt agccgccgga gctgccgcgc ctgcttcacc cgcgacttcg aagaccataa
    23641 tcagcgagcc ggtagacact ttgtcaccca cgttcacttt gatctctttc acggtgccag
    23701 caaacggagc cggaacttcc atagaagctt tatcgccttc tacggtgatc agcgactgtt
    23761 cagcttcaac tttatcgcca actttcacca ggatttcggt cacttcaact tcgtcgctgc
    23821 cgatatccgg aacgttaacg tcttttgccg ccgcagccgc gggtgctgct gccggagctg
    23881 cttctttctt ctcttctgcc tgagcaggtg cagcgtctgc tgcaccgtcg gcggaatcga
    23941 aaatcataat cagtgcgccg gtctgggttt tatcgccaac agagactttg atctctttaa
    24001 cgatacccgc ctgcggagac ggaacttcca tagaggcttt gtcgccttct acggtgatca
    24061 gcgactgttc ggcttcaact ttgtcgccca ctttgaccag gatctcggtg atttcaactt
    24121 catcagcccc gatgtccggt actttgattt cgatagccat tattctttta cctcttacgc
    24181 cagacgcggg ttaactttat ctgcatcgat gttgaatttg gcgattgcgt cagcaaccac
    24241 tttcttatcg atttcgccac gtttagccag ttcgcccagc gccgcaacca cgacataaga
    24301 agcatcaact tcgaagtggt gacgcaggtt ctcacggctg tcggaacgac cgaagccatc
    24361 agtacccagt acgcggtagt cgtcagccgg tacgtaagta cggacctgct cagcgaacag
    24421 tttcatatag tcggtagatg ccactgccgg agcgtcgttc atcacctgag cgatatacgg
    24481 aacgcgcgga gtttccagcg gatgaagcat gttccagcgt tcacaatcct gaccatcacg
    24541 cgccagctcg gtgaaggagg tcacgctata aacgtcagaa cctacgccgt aatctttcgc
    24601 caggatctct gccgcttcac ggacgtgacg caggatagaa ccggagccca gcagctgaac
    24661 tttacctttg ctaccttcaa tggtttcgag tttgtagata cctttacgga taccttcctc
    24721 agcaccttcc ggcattgccg gcatgtggta gttttcgttc agcgtggtga tgtagtagta
    24781 aacgttctct tgtttttcac cgtacatacg ctccagaccg tcatgcataa tgacagcaac
    24841 ttcgtaagcg taagccgggt cgtaagagat acagttcgga atagtcagcg actgaatgtg
    24901 gctgtgacca tcttcatgct gcagaccttc tccgttcagg gtggtacgac cggaagtacc
    24961 gccgatcagg aagccacgcg cttgctggtc gccagccgcc cagcacagat cgccaatacg
    25021 ctggaagccg aacatcgagt aatagatgta gaacgggatc atcggcagat tgttggtgct
    25081 gtaagaggtc gccgctgcca gccaggaaca acctgcgccc agctcgttga tcccttcctg
    25141 cagaatctga cctttctcgt cttctttata gtaagcaacc tgctcgcggt cctgcggggt
    25201 gtactgctga ccgttcgggc tgtaaatacc aatctgacgg aacagacctt ccataccgaa
    25261 agtacgcgct tcgtcggcga tgatcggtac cagacgatct ttgatcgact tgttcttcag
    25321 cattacgttc agggcacgaa cgaaagcgat agtggtagag atctctttgc tctgctcttc
    25381 cagcagcgcg ccgaagtctt gcaggctcgg cagctcaagc ttctcggtga agttcggctg
    25441 acggcttggc agataaccgt gcagtttctg acgctgtgcg tgcagatagg tatgctcttc
    25501 agaaccttcc gggaaggtga tgtacggcag tttttcgata tctgcatcag acaccggcac
    25561 attgaaacgg tcgcggatgt gacgcacgcc gtccatgttc attttcttaa cctggtgcgc
    25621 gatgttttta ccttcagccg cgtcgcccat gccgtaacct ttaatggaat gagcaaggat
    25681 tactgtcgct ttgcctttgg tttcctgcgc tttcttgaat gcagcgtaga ttttcttcgg
    25741 atcgtgacca ccacggttca gtgcccagat ctgctcgtca gtccagtctg caaccagtgc
    25801 tgcggtttca ggatatttac cgaagaagtg ttcacgaacg tacgcaccat ctttcgattt
    25861 gaaggtctgg tagtcgccgt caacggtttc gttcatcagc tggatcagtt taccgctggt
    25921 atctttacgc agcagttcat cccaacggct accccacatc actttgatca cgttccagcc
    25981 agcaccttcg aagatgcctt ccagttcgtt gatgatcttg ccgttaccgg tgaccgggcc
    26041 gtcaagacgc tgcaggttac agttgataac gaagaccagg ttatccagtt tttcacgggt
    26101 agcgatggtg atcgcacctt tggattccgg ttcgtccatt tcaccgtcgc cgaggaacgc
    26161 gtaaacggtc tgtttagagg tatctttcag gccacggtgt tccagatatt tcaggaattt
    26221 agcctggtaa atagcaccaa tcggccccag ccccatagat acggtcggga actgccagaa
    26281 ttccggcatc agtttcgggt gcggatagga agagaggcca ttgccgtgaa cttcctgacg
    26341 gaagttatcc agctgctcct gagtcagacg accttccagg aaagcacgag cgtacacgcc
    26401 cggggagatg tggccctgga agtaaaccag gtcgccgcca tcctgctcgt tgcgtgcacg
    26461 gaagaagtgg ttaaagcaca catcataaat ggttgcggaa gactggaagg acgccatatg
    26521 gccgcccagt tcgaggtctt ttttcgacgc acgcagcacc gtcatgatgg cgttccagcg
    26581 gatagctgaa cgaatacggc gttccagttc cagattaccc ggatactccg gttgttcttc
    26641 aacggggatg gtgttgatgt agttgctgat acctgtgcct gcggctacgt ttacaccgcc
    26701 tttgcgggct tcagcaagca gttggtcgat cagatactga gcacgctcaa caccttcttc
    26761 acggatgacc gattcgatcg cctggagcca gtcgcgagtt tcgatcggat ccacgtcatt
    26821 tgggaaacgt tctgacatgg gttattcctt atctatctaa taacgttgag ttttctggaa
    26881 cctgtcccat tgaactctcg ccggaaagct caataagaca ggttctacgt ttagttgccg
    26941 cgcttttata tgcgcttgat ttacaacatc ttctggataa tttttaccag taaaatcact
    27001 aattctttcg ttgctccaga cgacgcagag aacgctcacg gcggctctct tcacgacttc
    27061 tgtcgagcaa aatttcttcg ataaaggcca gatggcgatg cgatgcttcg cgcgcttctt
    27121 ccggcttacc ggccataatc gcttcaaata tgcgggtgcg gtgactactc accagcggca
    27181 gcatctcgcg acgcgaatag agcaattcga agttctggcg gacattctgg gccaacatcg
    27241 gctccataca ccttagcaga tgaagcagaa ccacattgtg ggccgcttca gtgacagcaa
    27301 tctgatactg gagtacggcg tttgattccg cgtccagatc gccagactgc tgcgccagtt
    27361 ctatggcgtg atggagttca cggatgcgtt ccttgtcttc atcggtacta cgcagcgcgg
    27421 cgtaataagc ggcgatacct tccagggcgt gtcgtgtttc gagcaagtca tactgtgact
    27481 caggatggtc ggagagcagc tccaccagcg gatcgctgaa gctttgccat aggctgctct
    27541 ggacaaaagt gccgccaccc tggcgacgaa gcaacaagcc cttcgcttcg agacgttgaa
    27601 tcgcctcacg caaggaggga cgggagacgt caaactgttt tgccagttcg cgttccggtg
    27661 ggagtttttc gcccgggcgg agagtgcctt cgaggatcaa aaactccagt tgctgctcaa
    27721 tcacatcgga gagttttggt tggcggattt tgctgtaggc catgagttcc tgtcttaagc
    27781 cacttgccga agtcaattgg tcttaccaat ttcatgtctg tgacgctaaa gtaacaaagt
    27841 attcacctta tgtccataca ggttttgatt gaaatcatga aactgtgcac attttaacaa
    27901 cttgacatat ataacgtttc aaagttgtaa ctatgcacaa atgtagactt tacgtaggaa
    27961 agggattttt taacgattat taaactataa aaatccgaat tgaaccgatt cacttaccaa
    28021 ttttgtgatt tttaattcaa ttaaaacgaa tttaaattca ttctacatat tgagaggggt
    28081 tgaggctgag ctttacaaac ggtttctttt taagcaactc atcttcaacc atgcataaag
    28141 cgggtgcatt cgctgccgca taccattatt cttgatctga cggaagtctt tttgtaacaa
    28201 ttcaaacttc tttgatgtaa acaaattaat acaacaaacg gaattgcaaa cttacacacg
    28261 catcactgcg tagatcaaaa aaacaaccac cgcacgaggt ttcatgatgg aaggtcaaca
    28321 gcacggcgag cagctaaaac gcggccttaa aaaccgccat attcagctta tcgcgctggg
    28381 tggcgcgata gggaccgggt tattcctggg tagcgcctcc gtaatacagt ccgcagggcc
    28441 agggattatc ctgggttacg ccattgctgg ttttatcgcc tttctgatca tgcgtcagct
    28501 gggtgaaatg gtggtcgaag aacctgtcgc aggctccttt agccactttg cttataaata
    28561 ctggggcagt tttgccggtt tcgcctctgg ctggaactac tgggtactgt acgttttagt
    28621 tgccatggct gagctgactg ccgtgggtaa atacattcag ttctggtatc cggaaatccc
    28681 cacctgggtt tctgccgccg tattctttgt ggtgattaac gccatcaacc tgaccaacgt
    28741 taaagtgttt ggcgagatgg agttctggtt tgccattatc aaagttatcg ctgtggtagc
    28801 gatgatcatc ttcggcggct ggctgctatt cagtggcaac agcggcccgc aggcgaccgt
    28861 tagcaacctg tgggatcagg gtggtttcct gccgcacggc ttcaccgggc tggtgatgat
    28921 gatggcgatt atcatgttct cgttcggtgg tctggaactg gtggggatca ccgcagcaga
    28981 agctgataac ccggagcaaa gtataccgaa agcaactaac caggttatct accgcatcct
    29041 gattttctat attggttcgt tagccgttct gctctcactg atgccgtgga cccgcgttac
    29101 cgccgatacc agtccgtttg tgctgatctt ccacgagtta ggcgatacct ttgtggcgaa
    29161 tgcgctgaac atcgtggtac tgactgcggc gctctccgtg tacaacagct gcgtatattg
    29221 caacagccgt atgctgtttg gtctggcaca acagggtaat gcgccaaaag cgctggcgtc
    29281 tgtcgataaa cgtggtgtac cagtaaatac cattctggtg tctgcactgg taacggcgtt
    29341 gtgcgtactg attaactacc ttgccccaga gtccgctttc ggactgttaa tggcgctggt
    29401 ggtatctgca ctggtaatca actgggcgat gattagcctg gcgcatatga aattccgtcg
    29461 cgccaagcag gaacaaggcg tggtaactcg cttccctgct ctgctttatc cgctgggtaa
    29521 ctggatctgc ctgctgttta tggcggcggt actggtgatt atgctgatga ccccaggaat
    29581 ggcgatttcg gtatacctga tcccggtatg gctgatcgtg ttaggtatcg gctatctgtt
    29641 taaagagaaa accgccaaag ccgtaaaagc gcattaatct ctctacgccc tcacccgtac
    29701 agggtgaggg caataatctt tacaccaacg ccccgtaaat cgtcagtagt gcaatcacca
    29761 ccacgaccac gaacgaggtt ttcttcgcca ttgaaaccgc tgccttcggc gtctccacct
    29821 tatcgacatg cggttcacgc gccagagaga actgcgccag acgcgttaac acctgatact
    29881 gcgaagtatg gaagtcaccc agcgaagcaa accaggccgg taacgctttc tccccatgac
    29941 cgatcaaggc atataccaca cccgcaagac gaaccggcac ccaatccagt acatgaagca
    30001 cggcatcaat gccggactgt aaacgatgat gcggcgtctg gtatcgcgcc agccagtatt
    30061 gccatgcacg caaaaacgca taccccatca gcgtaacggg tccccagatt ccccccacaa
    30121 tcaaccagaa cagcggagca agataaaaac gaaagttaat ccacagcaat gcattttgca
    30181 gctcacgcaa atactcacgt tcatcgcagc ctgccgggac gccgtgaatc atggtgagtt
    30241 cgccagccat cgtgacacgg gcatggctat cattacgtga agcagctgtc agataagcat
    30301 gataatgaag acgaacttta cctgcgccaa tacacagcaa accaatcagc agccacacca
    30361 gcagcgtggg aacgttgaac aatactccct gcaatgcgcg taacagtaaa aaagtcacgc
    30421 ccatcgcaat aatggtcatg cctaacgtgc gcccgagaga aaaatgtttc acccgccgaa
    30481 agaacgcttc aagacgatga tcaagctgcc agtgctcgcc caacttaaac aggcgctcga
    30541 aaattaacac cagtaaggtt gtaaatagcg tcatgttgtc tccttgctga ccagcgcacg
    30601 aaaccgcgcc caatcaaatg cagggccggg atcggttttc cgatccggcg caatatcaca
    30661 atggcccgtc atgttattag cgatatctgg atagcgatca atcagtgccc gcgtaaccgc
    30721 cgcaagctgt tgatactgcg catcggtata cgccagcgta tcggtgcctt caagctcaat
    30781 cccaatagaa aaatcattgc agcgttcgcg cccctgatac tgagagactc ccgcatgcca
    30841 tgcacgttta tcgaaaggaa catactggac tatttcacca tcacggcgaa tcaaacagtg
    30901 agcggagacg cgcaaatggg cgatctcagc aaagaaagga tgtgcctgcg gatcaatagt
    30961 tccagtgaat aatgcgtcga tccacggacc gccaaactcg cctggcggca ggctaatatt
    31021 gtgcaccacc agcagggtgg gtgtttcgtc atccgggcgg caatcgtaat gtggtgaggg
    31081 aacgcggcgc gcgccaacca gccacccctg ttctaacaac atgcaggatc tccttatatg
    31141 tggtgctaat acccggttca gagtagcatg tttctacctt atgattcgtt agctatctgg
    31201 agttttaaca tgccgcctcg ccgctataac cctgacaccc gacgtgacga gctgctggaa
    31261 cgcattaatc tcgatatccc cggcgcggtg gcccaggcgc tgcgggaaga tttaggcgga
    31321 acagtcgatg ccaacaatga cattacggca aaacttttac cggaaaactc tcgctctcat
    31381 gccacggtga tcacccgcga gaatggcgta ttttgcggca aacgctgggt tgaagaggtg
    31441 tttattcaac tggcaggcga cgatgtcacc ataatctggc atgtggatga cggcgatgtc
    31501 atcaacgcca atcaaccctt gttcgaactt gaaggcccat cccgcgtgct gttaacgggc
    31561 gaacgcactg cgcttaattt tgtgcaaacc ctttcaggag ttgccagtaa ggtacgccac
    31621 tatgtcgaat tactggaagg caccaacacg cagttgttgg atacgcgcaa aaccttacct
    31681 ggcctgcgtt cagctctgaa atacgcggta ctttgcggcg gcggagcgaa tcaccgtctg
    31741 gggctttctg atgccttcct gatcaaagaa aatcatatta ttgcctccgg ctcagtgcgc
    31801 caggccgtcg aaaaagcgtc ctggctgcac ccggatgcgc cagtagaagt cgaagtagag
    31861 aatctggaag aacttgatga agccctgaaa gcaggagccg atatcatcat gctggataac
    31921 ttcgaaacag aacagatgcg cgaagccgtc aaacgcacca acggcaaggc gctactggaa
    31981 gtgtctggca acgtcactga caaaacactg cgcgaatttg ccgaaacggg cgtggacttt
    32041 atctccgtcg gtgcgctaac taaacacgta caagcactcg acctttcaat gcgttttcgc
    32101 taattgctga cagatttatc cgctccatcg cgggcggata atctcccctt ccccaacttt
    32161 cttcgtaacg cctcgcaaat ttcttgtctt tcgctgcaac gaaataaaaa acctcggaag
    32221 ctgctttccc gtttcgtttt ttgcccctcg cttgtgctga tggcgtttgc caaagtagcg
    32281 ccaaccaaat caaggagcga aacagatgga caagcaacgc ggttttacac ttatcgaact
    32341 gatggtggtt attggcatca ttgccatttt aagcgccatt ggtattcccg cttatcaaaa
    32401 ctacctgcgc aaagctgcac tcaccgacat gctacaaacc tttgtgcctt accgtaccgc
    32461 cgtagagttg tgcgcgctgg aacatggtg
//
LOCUS       DADLFA010000027        31370 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000027 DADLFA010000000
VERSION     DADLFA010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31370
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.027"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(15..3296)
                     /locus_tag="JQH65_001060"
     CDS             complement(15..3296)
                     /locus_tag="JQH65_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HAY6138546.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISCYKNVPVTTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSKRGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLLDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARV
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAIAFWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            3414..4607
                     /locus_tag="JQH65_001061"
     CDS             3414..4607
                     /locus_tag="JQH65_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAY6138547.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEKKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(4671..5924)
                     /locus_tag="JQH65_001062"
     CDS             complement(4671..5924)
                     /locus_tag="JQH65_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAY6138548.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            6252..7442
                     /gene="hmpA"
                     /locus_tag="JQH65_001063"
     CDS             6252..7442
                     /gene="hmpA"
                     /locus_tag="JQH65_001063"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HAY6138549.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISSGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIYYECFGP
                     HKVL"
     gene            complement(7487..7825)
                     /gene="glnB"
                     /locus_tag="JQH65_001064"
     CDS             complement(7487..7825)
                     /gene="glnB"
                     /locus_tag="JQH65_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HAY6138550.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(7886..9220)
                     /gene="glrR"
                     /locus_tag="JQH65_001065"
     CDS             complement(7886..9220)
                     /gene="glrR"
                     /locus_tag="JQH65_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HAY6138551.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(9210..9923)
                     /gene="qseG"
                     /locus_tag="JQH65_001066"
     CDS             complement(9210..9923)
                     /gene="qseG"
                     /locus_tag="JQH65_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HAY6138552.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGCWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(10088..11515)
                     /gene="qseE"
                     /locus_tag="JQH65_001067"
     CDS             complement(10088..11515)
                     /gene="qseE"
                     /locus_tag="JQH65_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HAY6138553.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(12073..15960)
                     /gene="purL"
                     /locus_tag="JQH65_001068"
                     /gene_synonym="purI"
     CDS             complement(12073..15960)
                     /gene="purL"
                     /locus_tag="JQH65_001068"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HAY6138554.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRILGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRILHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            16219..17775
                     /gene="mltF"
                     /locus_tag="JQH65_001069"
     CDS             16219..17775
                     /gene="mltF"
                     /locus_tag="JQH65_001069"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HAY6138555.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLCNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(17772..18308)
                     /gene="tadA"
                     /locus_tag="JQH65_001070"
     CDS             complement(17772..18308)
                     /gene="tadA"
                     /locus_tag="JQH65_001070"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAY6138556.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(18333..18968)
                     /gene="yfhb"
                     /locus_tag="JQH65_001071"
     CDS             complement(18333..18968)
                     /gene="yfhb"
                     /locus_tag="JQH65_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HAY6138557.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDKPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            19177..20025
                     /locus_tag="JQH65_001072"
     CDS             19177..20025
                     /locus_tag="JQH65_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAY6138558.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            20081..20341
                     /gene="yfhL"
                     /locus_tag="JQH65_001073"
     CDS             20081..20341
                     /gene="yfhL"
                     /locus_tag="JQH65_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HAY6138559.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            complement(20535..20615)
                     /gene="shoB"
                     /locus_tag="JQH65_001074"
     CDS             complement(20535..20615)
                     /gene="shoB"
                     /locus_tag="JQH65_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HAY6138560.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(21035..21415)
                     /locus_tag="JQH65_001075"
     CDS             complement(21035..21415)
                     /locus_tag="JQH65_001075"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAY6138561.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            complement(21415..22146)
                     /gene="pdxJ"
                     /locus_tag="JQH65_001076"
     CDS             complement(21415..22146)
                     /gene="pdxJ"
                     /locus_tag="JQH65_001076"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HAY6138562.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLTDAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(22158..22886)
                     /gene="recO"
                     /locus_tag="JQH65_001077"
     CDS             complement(22158..22886)
                     /gene="recO"
                     /locus_tag="JQH65_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAY6138563.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTYCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(22898..23803)
                     /gene="era"
                     /locus_tag="JQH65_001078"
     CDS             complement(22898..23803)
                     /gene="era"
                     /locus_tag="JQH65_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAY6138564.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(23800..24480)
                     /gene="rnc"
                     /locus_tag="JQH65_001079"
     CDS             complement(23800..24480)
                     /gene="rnc"
                     /locus_tag="JQH65_001079"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003848606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAY6138565.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(24753..25727)
                     /gene="lepB"
                     /locus_tag="JQH65_001080"
     CDS             complement(24753..25727)
                     /gene="lepB"
                     /locus_tag="JQH65_001080"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAY6138566.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(25743..27542)
                     /gene="lepA"
                     /locus_tag="JQH65_001081"
     CDS             complement(25743..27542)
                     /gene="lepA"
                     /locus_tag="JQH65_001081"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010434076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAY6138567.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(27740..28219)
                     /gene="rseC"
                     /locus_tag="JQH65_001082"
     CDS             complement(27740..28219)
                     /gene="rseC"
                     /locus_tag="JQH65_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HAY6138568.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(28216..29172)
                     /gene="rseB"
                     /locus_tag="JQH65_001083"
     CDS             complement(28216..29172)
                     /gene="rseB"
                     /locus_tag="JQH65_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HAY6138569.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGDEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(29172..29822)
                     /gene="rseA"
                     /locus_tag="JQH65_001084"
     CDS             complement(29172..29822)
                     /gene="rseA"
                     /locus_tag="JQH65_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HAY6138570.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(29855..30430)
                     /gene="rpoE"
                     /locus_tag="JQH65_001085"
     CDS             complement(29855..30430)
                     /gene="rpoE"
                     /locus_tag="JQH65_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HAY6138571.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(30427..30582)
                     /gene="rseD"
                     /locus_tag="JQH65_001086"
     CDS             complement(30427..30582)
                     /gene="rseD"
                     /locus_tag="JQH65_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HAY6138572.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            30838..>31370
                     /locus_tag="JQH65_001087"
     CDS             30838..>31370
                     /locus_tag="JQH65_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY6138573.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRR"
BASE COUNT         7502 a         8403 c         7917 g         7548 t
ORIGIN      
        1 acagttaata gggtttagtg aacgtagtta aaaataaaag gcatcacagt ggtgaataat
       61 gccgctttcg gccaggccgg attaatcgcc gtcaattcag cgcgagcaga ctcatattgc
      121 gcaacctccc cgagtcccgc ggacgccagc atcgccacca tcaggcattt ttctttatgc
      181 tgctgttgta aatcgccata cagcgataac aggtcaggtt gtgaaacagc aaagaaatcc
      241 gcctcaatac tggttttcgc catctcttgc gcccattgtt tcatttcact aaacagttgc
      301 tgtgcggttt gctgttcacc cagcagtcgc agcgccattc cttgccagaa gagataatca
      361 accggctgat cgttgtaata actgtggatg ttaatggtgc gatcgccggt cgccgccaga
      421 cgtaaacaac gcatcgcttc agtttcatcg ccctgcgcgt tggcgcatat cgcctgccag
      481 aaccagatgt cgttatcagt ttgccccggt aaacggcctt cgcttaaatt ctccggataa
      541 tgcagcgcgg catgaagcag ttcgctggcc tgctgcggct ctctggcatc aagatgctgc
      601 caggcgcgta ataactggtt gaggataaac tgactggtga ccttcccttc cccgccttcc
      661 cacgggtgga atttacgcgt ggcgagaatg tccgccgctt tgtctgcctg acccgtgaga
      721 tgccacaaat tgagcagttc tgcggtcatg tcgtcgcgtt tcagcgcaat ttccagatta
      781 ttttccagac gcgccagtcg tttctccggt gtggctccac ttaacttatc aagcaaatcc
      841 cgttcgaaaa gcagacgtgc atcctgcggc gcaagctgat aagcattatc aagataacgc
      901 gcggccagct cataatcgtg ttgcttattc cacgcatgga tcgctaaccc gcgccagccg
      961 tcggcaaact ccggcgacat ctctacgcaa cgttgccaaa aggcaatggc tttgttgtag
     1021 ctacgtttgt tgtagtagaa gcaggccagt aaatggcgag caaaccagca ctcttcaata
     1081 ctctccagcg ccgccacttc ttccagcgta ttcgggaaac ggacaaactg cgggaagaca
     1141 tcaatggctt ttgcgaccag ttcgccacgt tcggctttcg gcagcaagct ggcttgcagg
     1201 taaagcggca gcgtgcgctg gcagtccagt gcgttcagca tctctgccgc cagggtgggc
     1261 attccccagt taatcagttg cccggcggtc atcagggcgt taacgtcgcg tccctgacac
     1321 agaccgcgcc actgcgcgag agctgattcg ctacgaccat cgaaccagtt cagccaccac
     1381 agagtggcgt tcagcggata atcgcgcagc agtttctcgc gctgcacacg cgcgttgtcc
     1441 tgacgaccac ttaacaccag cagcagatta tgcaggcaaa gcacttcctg attggttggg
     1501 caggcgcgaa gactttgttg gcaaaaatcc agaccagcgt cgaagttacc attacgcgcc
     1561 gccagtcgtg ccaggccata atagccaccg gctttgctgt tgccgctcca gaccgcacgc
     1621 cagaaatcct cttcggcttg ttgatattgt ccctgacgtt cgtaagcact ggcgcgaatc
     1681 aaactcgcct gtccgcactg cggatttttg ttcagcgcat gtgcgcgttt cagagcctga
     1741 gtggcatacg ccaccgcttg cgggaaatcg gcgcggttat attccagcat cgccagcgcc
     1801 aggttacagc ggtaatccag cggatccagc gccacgccgc gcaggtagta atcgaacggt
     1861 gaacggctgg cgtgatgata ttgctccaga tgctgaccga taaaccaggc ttcatctgta
     1921 ctggtaatgt cttgtgctgc cagtggcgct ttggcgacgt ccagcaacgg caactcttgc
     1981 ggctgatgtt cctgataact cagtacaata ttgccgtcgg catcggagag ctcaatggtc
     2041 agcctttcag gattgatacc gtgcaacacg ccctggatgg cggtcgcagg catcagtgcc
     2101 acggcatcat caagtaacgc gttgcatttg ccgatttcgc ggatcgccag gcggtatccg
     2161 ttcaacggag agatggcata cagcccccac tcaatccccc gcttactacg ctggagtttt
     2221 atcaccgcat cgcgggaggc attttgcacc atgcccaaag aatgataagg caggaaatac
     2281 tgctcaaaac gcttctcttc gtaagcatca agccaggtaa aatcgggctg gttatcggca
     2341 aaaataccgg tcatcagttc gatatacggg ccgttattgt cggtcagact cttatcccac
     2401 gcctggccaa attcactgtg tccccaactc cactgttttt tacctggcgc aatatggtgg
     2461 ttggcaacgt gcagcaaacc gccatcttca tcgtgacacc acgcgccgac aaaatcgtac
     2521 tgcgattttt cggccatata tgaggttgta acaggcacat ttttatagca agaaatgtcc
     2581 actccggcgg agtagtccac tttgtagtaa gtgccggtgg cgatggggaa agcggagacg
     2641 gcccgtttgc cgtgatcaaa caccgcagtc acatccggtg ggaaaacgct ctgatgccct
     2701 tccccccctt tcactgccgg gttggcccac cacaagaaat gacgcggcgt ggcgttaccg
     2761 ttatagacgc ggctggcgat ttccagcgcc gcccggtcag ggcgcagggt gaaacctgtc
     2821 atcacctgta aaccatgcat cggctccgtt tcgcctaccc acaccgtctg tgcaccgtct
     2881 tcatgggctt cgagggtgaa atcaacgggc ataaaggtgg tcgggcgatg atgttgcggc
     2941 cagttaaact caatcccacc ggaaatccac ggccccagca gccccaccag cgcaggttta
     3001 atgacttcat tgtgataaac aaaatcgcgc tgtttcactt tatcccacgc gcgatgcacc
     3061 cgaccgccca gttccggcag gatcatcact ttgatgtagt cgttttccag ccacaccgcc
     3121 tgccaggatt tcagggtttt ctgctcgctc agcgtatcgg tcacgccgta gggataaacc
     3181 gcgccggacg atccctgata aacgcgattt tccaggaaca tgggatgtat atcctgcggc
     3241 ccggtttcat aggtcgggat ctcaacgcgc tcttgccaca cttttactgg agtcatggtg
     3301 ccctcaatat taacaagaca tactgaatta aaagatttgt ggcagtgtat tgaacaatct
     3361 ggcaatgttt tcgcggaata atcacgcaat taactaaaca aggtttagtg aagatgagag
     3421 cctgcattaa caatcaacag attcgccacc ataacaaatg cgtgattctg gaactgctgt
     3481 accggcaaaa gcgcgccaat aaatcaacgc tggcccggct ggcgcaaatt tcgattccgg
     3541 cagtcagtaa tattttgcag gaactggaaa gcgaaaaacg ggtggtgaat attgacgatg
     3601 aaagccagac gcgcgggcat agtagcggta catggctgat tgcgccggaa ggtgactgga
     3661 cgctgtgcct gaacgtgacg cccaccagta ttgagtgtca ggttgctaat gcttgtttaa
     3721 gtccgaaagg tgaatttgag tatttacaga ttgatgcacc gacgccgcag gcgctgctgt
     3781 ccgaaatcga aaaatgctgg catcgccacc gtaaattgtg gccggaccgc accatcaatc
     3841 tggcgctggc aatccacggt caggttgatc cggtgaccgg cgtgtcgcaa accatgccgc
     3901 aagcgccgtg gacaacgccg gttgaggtga agtatctgct ggaaaagaag ctgggcattc
     3961 gggtgatggt cgataatgac tgcgtgatgc tggcgctggc ggagaaatgg caaaataatt
     4021 cgcaggaacg ggatttctgc gtgatcaacg ttgattacgg cattggctcg tcgttcgtga
     4081 ttaacgagca aatttatcgc ggcagtttgt atggtagcgg acaaattggt cacaccatcg
     4141 ttaatccgga tggcgtcgtc tgcgactgtg gacgttacgg ctgcctggaa accgtcgcct
     4201 cgttaagtgc attaaaaaaa caggcgcggg tatggctaaa atcacaaccg gttaatactc
     4261 agcttgatcc tgaaaaactg acaaccgcgc agttaatcgc tgcctggcag agtggagaac
     4321 cgtggatcac cagctgggtt gatcgctctg ccaatgccat tggtttgagt ctgtataact
     4381 tcctcaacat cctcaatatt aatcagattt ggttgtacgg tcgcagttgt gcctttggtg
     4441 agaactggct taatactatt attcgccaga caggatttaa cccgttcgac cgcgacgaag
     4501 gaccgagcgt gaaagcgacg caaattggct tcgggcaatt aagccgcgca caacaggtgc
     4561 tgggaattgg ctatttgtat gttgaggcgc agttacgaca gatttgatgg cgcgataacg
     4621 tagaaaggct tcccgaagga agcctttttt caacagatta accgtttcgc ttatgcgtaa
     4681 accgggtaac gtgcgcagat gtcgagaact ttacctttga tgcgctcgat aacggcttca
     4741 tcattgatgc tgtccagcac gtcacacatc cagccagcca gttctttcgc ttctgcttct
     4801 ttgaagccgc gacgcgtaat tgccggagta cccacgcgaa taccggaggt cacaaacggg
     4861 ctcttcggat cgttcggtac gctgtttttg ttgacggtga tgttagcacg gcccagagcg
     4921 gcgtctgctt ctttaccggt caggttttta tcaaccagat caaccaggaa caggtggtta
     4981 tcagtgccgc cggaaaccac tttgtagccg cgctcgagga acacttctac catcgcttta
     5041 gcgtttttcg cgacctgctg ctggtaagtt ttgaactcag gctccatcgc ttctttcaga
     5101 gcaaccgctt taccggcgat tacgtgcatc aacggaccgc cctgaccacc agggaaaacg
     5161 gcagagttca gttttttgta cagctcttcg ctaccacctt tcgccaggat caggccgccg
     5221 cgcggacccg ccagggtttt gtgagtggtg gtagtaacaa cgtgagcatg aggaaccggg
     5281 ttcgggtaga cgccagcagc aaccaggccc gcaacgtgcg ccatatcaac gaacaggtaa
     5341 gcaccgatgc tgtcagcgat ttcacgcatt ttcgcccagt ccaccacgcc ggaatatgca
     5401 gagaaaccac cgataatcat tttcggcttg tgttctttgg cttgtttttc cagatcggcg
     5461 tagtcgatat gaccggtagc atcgataccg taaggaacga tgttgtacag tttaccggag
     5521 aagttaaccg gagaaccgtg agtcaggtga ccgccatgcg ccaggttcat acccagaacg
     5581 gtatcacctg gttccagcag cgcggtgtag accgcaaagt tagcctggga gccggagtgc
     5641 ggctggacgt tagcgtagtc agcgccgaac agttctttcg cacgatcgat cgccagttgt
     5701 tcaacgatat caacatactc gcaaccgccg tagtagcgtt tgcccggata accttcagca
     5761 tatttgttgg tcagctgaga accctgcgcc tgcattacgc gcgggctggt gtagttttcg
     5821 gaggcgatca gttcgatgtg ctcttcctga cgtacttttt cctgctccat agcctgccac
     5881 agttcggcat cataatcggc aatgttcatt tcacgcttta acatccgcat ctcctgactc
     5941 agctaacaat aaaatttttg gcctttatag gcggtcctgt tggacaacgg cgaacagtat
     6001 aaccgaatca ttgtgcgata acaggtcttg acaaaggaat ttacgcaaac gattaccttc
     6061 aggctacgca aggctttgga gaataaagag cttgcaaccg gaaacggatt tcttttcagg
     6121 tttgtgatgc aaatttttca cttcatcaca ttctttctga aaaacaccaa agaaccattt
     6181 acattgcagg gctatttttt ataagatgca tttgaaatac atcaattaag atgcaaaaaa
     6241 aggaagacca tatgcttgac gctcaaacca tcgctacagt aaaagccacc atccctttac
     6301 tggtggaaac ggggccaaag ttaaccgccc atttctacga ccgtatgttt actcataacc
     6361 cagaactcaa agaaattttt aacatgagta accagcgtaa tggcgatcaa cgtgaagccc
     6421 tgtttaacgc tattgccgcc tacgccagta atattgaaaa cctgcctgcg ctgctgccag
     6481 cggtagaaaa aatcgcgcag aagcacacca gcttccagat caaaccggaa cagtacaaca
     6541 tcgtcggtga acacctgttg gcaacgctgg acgaaatgtt cagcccgggg caggaagtgc
     6601 tggacgcgtg gggtaaagcc tatggtgtac tggctaatgt atttatcaat cgcgaggcgg
     6661 aaatctataa cgaaaacgcc agcaaagccg gtggttggga aggtactcgc gatttccgca
     6721 ttgtggctaa aacaccgcgc agcgcgctta tcaccagctt cgaactggag ccggtcgacg
     6781 gtggcgcagt ggcagaatac cgtccggggc aatatctcgg cgtctggctg aagccggaag
     6841 gtttcccgca tcaggaaatt cgtcagtact ctttgactcg caaaccggat ggcaaaggct
     6901 atcgtattgc ggtgaaacgc gaagagggtg ggcaggtatc caactggttg cacaatcacg
     6961 ccaatgttgg cgatgtcgtg aaactggtcg ctccggcagg tgatttcttt atggctgtcg
     7021 cagatgacac accagtgacg ttaatctctt ccggtgttgg tcaaacgcca atgctggcaa
     7081 tgctcgacac gctggcaaaa gcaggccaca cagcacaagt gaactggttc catgcggcag
     7141 aaaatggcga tgttcacgcc tttgccgatg aagttaagga actggggcag tcactgccgc
     7201 gctttaccgc gcacacctgg tatcgtcagc cgagcgaagc cgatcgcgct aaaggtcagt
     7261 ttgatagcga aggtctgatg gatttgagca aactggaagg tgcgttcagc gatccgacaa
     7321 tgcagttcta tctctgcggc ccggttggct tcatgcagtt taccgcgaaa cagttagtgg
     7381 atctgggcgt gaagcaggaa aacatttatt acgaatgctt tggcccgcat aaggtgctgt
     7441 aatttgatgt tgccggatgg aaacatccgg caacccttga cgcggtttaa attgccgcgt
     7501 cgtcctcttc accggtacgg atgcgaatga cccgtgccac gtcaaagacg aagattttac
     7561 cgtcaccgat tttgccggtt tgcgccgtgc gaataatggt atcgacacag gtatcgacaa
     7621 tgtcgtccgg tacgacaatc tcaattttca ctttcggcag aaaatccacc atatactccg
     7681 cgccgcggta cagctcggta tggcctttct ggcgaccaaa gcctttcact tcggtcaccg
     7741 tcatgccggt aataccgact tcggccagtg cttcgcggac atcgtccagc ttgaagggtt
     7801 ttataatcgc atcaatcttt ttcatgctat tccttgaaaa ggtcgcctgt cttttgatct
     7861 gctaaacgta acacataacg ccaattcatt ccttgaaatc gtttgcatcc agctcgtgtc
     7921 gggaaagcag tttataaaat tctgtccggt tgcgccccgc cattctcgcc gcgtgggtga
     7981 cgttgccttt ggtaatttgc agcagcttac gcaaatagtt gagttcaaac tgattacgtg
     8041 cctcaacaaa ggttggcagc gccgtgtttt caccctccag cgcctgctct actaatgcat
     8101 cgctaataac cggagatgag gtcagcgcca cgcactgttc aatcacgttg accaactggc
     8161 gcacattacc cggccagcta gcggtcatca ggcgtttcat cgcatcagtg gagaacgcgc
     8221 ggacaaacgg tttatgtcgc tctgccgcct ggcgcaacag gtgatttgcc agaagcggaa
     8281 tgtcttctgt gcgctctgcc agagccggaa ttttcaggct gacaacgttg aggcggtaat
     8341 agagatcttc gcggaattcc ccgcgcgcca tcgcttttgg cagatcacgg tgagtggcag
     8401 aaataatccg cacattgata tcaatatcgc ggttactgcc cagcgggcgc actttacgct
     8461 cctgcaacac gcgcagcagt ttgacctgta acggcgcagg catatcgcca atttcatcga
     8521 gaaatagcgt tccgccttcc gccgcctgga ataaaccttc gcgattgctg acagcgccag
     8581 taaacgcgcc acgcgcatga ccaaacagct ccgactccag caattgctcg ggcaatgcgc
     8641 cacagttaat agcaataaat ggtttgctgt tgcgcgggct ggcgttgtgg atagcctggg
     8701 cgaaaatctc tttcccggtg ccgctctgac cgttaatcaa aacgctgacg tctgattgcg
     8761 ccaccagccg cgcctgttcc agcaaacgca gcatcagcgg gctgcgggtg acaattgcct
     8821 cgcgccagcg ttcatcggtg gctggcgcgg attgctccag cgcatcgtca attgcctgat
     8881 atagcgcgtc tttgtcgaca ggcttggtga ggaaactaaa aacgccctgc tgtgttgcag
     8941 caacggcatc ggggatagaa ccatgcgcag taagaataat tactggcatt cccggctgca
     9001 ctttctggat ttcagcaaat agctgcatac cgtccatttc atccatccgc aggtcgctga
     9061 taactaaatc tactttttcg cgattcagta cccgtaatcc ttcagcgcca ctttccgccg
     9121 tgaccacact gtagccttcg ctggtcaggc gcaggccaag cagtttcagc aatcccggat
     9181 cgtcatcgac caataataaa tgcgcaggtt tatggctcat caggagtgac ctcatgggtg
     9241 gatggcgcgg gcttttcact ctcgtgcggc gtatccggcg agaaatttcc agccggtttg
     9301 cgggtcgaga gctggcgttc aatatcggtc aggttttcca gcttgcgggt ggtgagttcc
     9361 agttgctgct gtagaacgtg atgttgctgg cgcaatgtat ccagctcgct gtcgctggac
     9421 tgctggagtt tactgtaacg ttggcgctct tccgccagtt gcagttgcag cgcctgaccg
     9481 tcgcgccaga gttgatacag tgggcgaacc tgtgccggga tctcggtact taacgcttca
     9541 atacgcgcga ccagctggcg gcgctcatac ggcgtaattt tggcgtcggc gagcaaaatc
     9601 ccttgtttaa aggtattttg ccagcagccg tcgtcatatt ggcgagcttg ctgacgcgac
     9661 tgcgcaggca ttaaacgatc agcacaatcc atcgcccgca gccagtaaag cggattggtt
     9721 tcggttgatt taccttgcag cgcccagatg tcgctacatt cagtagaaag atagtcagcc
     9781 agttgataaa ccggaatttt ttcttctgct ggcgtatcaa tggctggctt attgtgattc
     9841 tgcacgcaac ccagcaatgc cagacatggc agccctgcca gccacagccg tcggggcaat
     9901 aatcgttgaa aaatgtgtcg catattcacc agacttaaag cctatcccag tgggcgtaat
     9961 tgttgcagac agtctggaca tggacagcgc ggagaaaccg gagcgtacat atcgtacgtg
    10021 aagatttcga gcactgcccg gagccgaact gacaaataaa atagcctgat aggataggct
    10081 gttagcatta tttcgtgttt ttcgacgacg gtaattcaat gcggaaacaa acgtcttgcc
    10141 cgctctcgtc gaccagatac agttcccctt gcatacggcg aatacaatcc ctggcaatgc
    10201 ttaatcccag accgctgccc ttcaccgccc cttttcgctg gtggcttccc tgaaaaaagg
    10261 gttcgaagat catggcgcgt tcctcttgcg gaatgggcgt gcctgtattg atgacatcaa
    10321 tataaacccg cgcaccatgt gaactgctgc gaaggcaaat gttaccggat tcagccccgt
    10381 agtgcaccgc attggagtaa agattatcca gtacgctcat cagcagcatt ggctccgcca
    10441 ggcaagctgt tgctttgaga tcgacgtcgg tatgcatcat tttagcccgt gcgggcaggc
    10501 tatgagcaga aaccactgtc tccaccagcg gtgctaattc aacattctcc agttccaccg
    10561 cactgtccgc ctgtttacgg ttgtaatcaa gcagttgttc gatcagtttt tgcaaattgc
    10621 ggctgctgct atcaagaatg ctcaccacct ctttttgctc tggcgtaagc ggcccgacaa
    10681 cctggtcagc cagtaattca gtgccctcgc gcatactcgc cagtggcgtt tttaattcat
    10741 gagataaatg tcttaaaaat tgatggcgtt gggattccag ccatgacagg cgctcactta
    10801 accaaagaat acgttgccca accgagcgta actcgctcgg tccactgaac gaaacgctat
    10861 tgcccagaga acgcccttcc cccagacggt tgatcatgcg ctcgatattt ttcaccggcc
    10921 cgataatcat ccgcgtgaaa agtagcacca tcaccagact caccagaaat agcaccagcg
    10981 attgccaacc aaaatattgc ccacgttcgg cgatttcacg ctgaagttgc tgcccacgag
    11041 agaacaccac tgtgcgcgtg gcctgcacca tttcggtatt ggcactggca aaggcttcaa
    11101 gacgcgcggc ggcggcagca tcgggaccac tgttgttaca ctgaagttgg gccagattgt
    11161 tcaaatcctg acgcaatgcc tggtaaagtt tatcgtctgg cagtacgcct gcgtgggcat
    11221 cgagcatttc gctgtaacgc ttgcgctggc tttgataaac cttcgcaagc gtagggtcgt
    11281 ccagcacgca atactgacgg tagctacgct ccatctccag cgccgcgttg gtcatcgctt
    11341 cactacgccg ggcatcaata agcgtagtgc ggttaaccag cgccgcctga tcgctaagcg
    11401 cattcaggct ttgccaggct tgccatgcca gcaccaacag gggtagtaga attagcaaaa
    11461 atgccagcat taccagttgt cgtaatgagc ggggaaaaac gggccagcgt ttcaaggtgt
    11521 tactctcgtc agacgcgaat agcctgatgc taaccgaggg gaagttcaga tacaacaaag
    11581 ccgggaatta cccggctttg ttatggaata aggcggtgcc taactcgacg tttcgcccga
    11641 tggttgatat agctacgctg atatcagaag ttggacggca ggcaccttgt tgtgcgtcat
    11701 tcgaatttta tgtagcacgt cccgaagggg ctgacataag tcggtgaatg agccactgct
    11761 tactattatg cagtaactgt gccaataaag aaaatagttt ggtaacgtac tgattatacg
    11821 ttgctttgag ggatttatgg cttcttcgct atttgaatgt aaatcagcca ctgtgtcgct
    11881 aaaaagagac agcttaagat aaacctatta gattacaata aaatcaatca cttaagtgtc
    11941 gccagaaagc gacacggtaa accacccatt gtcgtgattt acagacacaa aaaagctccc
    12001 ggagttggga gcttatgata gtggttggtg cttaatgccg catccgggct gcaaaaccaa
    12061 tgaactgatg aattacccca actgcttacg cgcattgcgg aaaatgcgca tccatgggcc
    12121 atcctcgccc cagttttccg gatgccagga gttgctgaca gtacggaaaa cacgttccgg
    12181 gtgcggcatc ataatggtga ctcgaccact ttcagtcgtg actgccgtaa taccgttcgg
    12241 tgaaccgttc gggttagccg ggtaggtttc agtgactttg ccgaagttat cgacatagcg
    12301 cagtgccacc agccctttgc tttccagtgc cgccagatgc gccgcatcac gcacttccac
    12361 gcgcccttca ccatgagaga cagcaatcgg catttgcgag cccaccatcc cctgcaacaa
    12421 cagagacggg ctttgggtta cttcaaccag gctgaaacgc gcttcaaagc gatcggaggt
    12481 attgcgcaca aaacgtggcc acaactcact acctgggatc agttcgcgca ggttagacat
    12541 catctggcaa ccgttacata cccccagcgc cagcgtttgc ggacggtgga agaaggttgc
    12601 aaactcatcg cgtacacggt cattgaacag gattgacttc gcccaacctt caccggcacc
    12661 cagcacatca ccgtaggaga aaccaccgca cgcgaccagg gcgtggaaat cttccaggcc
    12721 cgtgcgtccg gtcagcaggt cactcatatg cacgtcgata gcatcaaagc ctgcacggtg
    12781 gaaagctgcc gccatttcaa catgcgagtt cacgccctgc tcacgcagta cggcaacttt
    12841 cggacgtgcg ccagtggcaa tatacggtgc tgccacatct tcgttgatat cgaacgacag
    12901 ttttacattc aggcccggat cggcgtcgtt agatttcgcc tgatgctcct gatcggcaca
    12961 ctccgggttg tcacgcaggc gctgcatctg ccaggtagtt tctgcccacc agacacgcaa
    13021 cgtggtgcgg ctttcgctga atacagtctg cccgttggcg gtaatcacaa aacggtcacc
    13081 ggaaaccgcc tgccctacat aatggacaca atcagcaagc ccatgctgtg ccagtacgga
    13141 ctcgaccgct tcacggtcag cggcacgaac ctgaatcacc gcacccagtt cttcgttaaa
    13201 caacgccgcc aggcgatcgt cgcccagaga ggcaatatcc gcgttaatgc cgcaatgacc
    13261 agcaaaggcc atttccgcca gcgttaccag caggccgcca tcagagcggt cgtggtatgc
    13321 gagcagctta cgctgtgcaa ccagcgcctg aatcgcgtca tagaagcctt tcagttgcgc
    13381 gacgtcgcgt acatctgccg gtttgtcgcc aagctgacga taaacctgcg ccagcgccgt
    13441 tgcgcccagc gcgttattgc ctttgcccaa atcaatcagc aatagtgcgt tatcttcggt
    13501 agaaagctgt ggcgtgatgg tgtgacgcac atcttccacg cgggcaaatg cagaaatcac
    13561 cagcgacagc ggcgacgtca tttcgcgctc ttcgttacct tcctgccagc gggttttcat
    13621 cgacatagag tctttaccca ccgggatcgt caggcccagc gccggacaaa gctcttcgcc
    13681 cacggcttta acggcttcat acaggcccgc atcttcacca gggtggcctg ccgccgccat
    13741 ccagttggcg gaaagtttaa tgcgtttgat atcgccaatc tgtgttgcgg caatattggt
    13801 taacgcttca ccgaccgcca gacgggcaga ggcggcgaaa tccagcagcg caaccggcgc
    13861 acgctcgcca atcgccatcg cttcaccgta gtagctgtcg aggctggcgg tagtgaccgc
    13921 gcagttagcg accggcacct gccacggacc caccatctga tcgcgcgcta ccatgccagt
    13981 tacgctgcgg tcaccgatgg tcaccaggaa agttttctcc gccacggtcg gcagatgcag
    14041 tatgcgtttc accgcgtctg caatggtgat cccttcacgg accagcgcat cgcccttcgc
    14101 tttcagcgtt tgtacatcgc gggtcatctt cggcgtttta ccaagcagga cgtccagcgg
    14161 cagatcgatc ggctgattat caaaatgacg atcgtgtaga gaaagatgca gttcttcggt
    14221 cgcttcacca atcaccgcgt agggtgcgcg ctcacgctta cacagttcgt caaacagcgg
    14281 taattgatcg gcagcaaccg ccagcacgta gcgttcctgg gattcgttac accagatttc
    14341 cagcgggctc atgcctggct cgtcgctcag aatgtcgcgc agttcaaatt taccgccacg
    14401 cccgccgtcg ctcaccagtt ccggcatagc gttagaaaga ccgcctgcgc caacgtcgtg
    14461 gataaacagt attgggttgg catcaccaag ctgccagcaa cggtcaatca cttcctggca
    14521 gcgacgctcc atctccgggt tgtcgcgctg tacggaagca aagtcgaggt cggcatcaga
    14581 ctgaccagac gccatggaag acgccgcgcc gccgccaaga ccgatgttca ttgccgggcc
    14641 gccgagaacg accagcttcg cgccaacgtt gatctcgcct ttttgtacgt gatcggcgcg
    14701 aatgttgccg atcccgcccg ccagcatgat cggtttgtga taaccgcgca gctcttcgcc
    14761 gttgtggctg ttcacttttt cttcataagt acggaagtag ccgttcagtg ccggacgacc
    14821 aaattcgttg ttaaacgccg caccgcccag cgggccttcg gtcatgatgt ccagcgcggt
    14881 gacaatgcgc tcaggcttac cgaaatcttc ttcccacggc tgttcgaagc caagaattcg
    14941 caggttggaa acggagaaac caaccaaacc cgctttcggc ttcgcgccgc gcccggtggc
    15001 accttcatcg cggatttcac cgccggaacc ggtcgccgcg cccggccacg gagaaatcgc
    15061 cgtcgggtgg ttgtgagttt cgactttcat cagaatatgc gccggttcct gatggaaatc
    15121 atagcggccc gtttcgtggt cagcaaagta gcggcccact tcagaacctt ccattacggc
    15181 ggcgttatct ttataagcag agagaacgtg atctggcgtg gtttcgaaag tatttttgat
    15241 catcctgaac agcgatttcg gctgttgttc accgtcgata atccagtcgg cgttaaaaat
    15301 tttgtggcgg cagtgctcag agttcgcctg agcgaacata tacagctcga tgtcgttcgg
    15361 gttacgacca agctttgtga aagcatcctg cagatagtca atttcatctt ccgccagagc
    15421 caggccaaga cgcaggttag cgtcgatcag cgcctgacgg ccctgcccca gcaaatcaac
    15481 gcttgtgacc ggagtcggtt gatggtgggc aaacaactgc tctgcatcat ctaaatcaaa
    15541 aaagacagtt tccatcatgc ggtcgtgcag ttcagcggta acctgctgcc attgttcatt
    15601 ggtcagcgta ccggcttcta tatagtaagc aacgccgcgc tcaaggcggt ttacctgttg
    15661 tagcccgcag ttatgggcaa tatcggtcgc tttcgaagac cagggagaga tggtgccagg
    15721 acgcggggtc accagcagga gtttgccttg cggggcgtgg ctggcgagtg ccggaccata
    15781 tttcagcagg cgttcaagtt gtgcgtgctc atcatcgttt aacggcgcat tgaggtcagc
    15841 aaaatggaca tactcggcgt aaatattgtg aaccgggagc ctggcagcct gaaaacgtgc
    15901 cagcagtttg ttgattcgga atgccgacag tgcaggcgaa ccacgcagaa tttccatcat
    15961 aagtctctcg tcttctaagc tttcggtgta cccaaggggg gggaaacggg cgtcattata
    16021 aagaatctga tgcgctgacg aaaccgtttg cgtggaaata aaatcaccat cgtgaattag
    16081 caacgcgtgc cgccaatggc tgtaataagt tgccatctgg cgcaggttta cgcaaaatgc
    16141 cgctcattta tgagtaaacc tttcactatt attacgtttt ttcaagctgg gacgcgcacg
    16201 acacagagaa ttaactaatt gaaaaaatta aagattaatt atctgttcat cggcattctg
    16261 gcactgctgc tcgcggtcgc tctctggcca tccattccct ggtttggtaa agccgacaac
    16321 cgtatcgccg ccattcaagc gcggggagag ttgcgtgtga gcaccattca tactcccctg
    16381 acttataacg aaatcaacgg gaaacctttt ggcctggatt acgaactggc gaaacagttt
    16441 gccgattacc tcggcgtaaa actgaaagtg accgtgcggc agaatatcag ccagctgttt
    16501 gacgaccttg ataatggtaa cgccgacctg ctggcggcag gactggtcta taacagtgag
    16561 cgggtaaaaa attatcagcc tggccctacc tattattccg tgtcacaaca actggtttat
    16621 aaagtgggtc agtatcgccc acgtacgctg tgcaacctga cggcggagca actcaccgtt
    16681 gcaccgggtc atgtggtggt taacgatctc cagaccctga aagaaacaaa attcccggaa
    16741 ttaagctgga aggtagacga caaaaaaggc tctgcggaat taatggaaga tgtcatcgaa
    16801 ggaaaactcg attacaccat tgctgattct gtcgccatca gcctgtttca gcgcgttcac
    16861 ccggagctcg ccgtagcgct cgatatcacc gatgaacaac cggtgacttg gtttagcccg
    16921 ttagatggcg ataataccct ttccgccgcc ctgctcgact tcttcaacga aatgaatgaa
    16981 gacggtacgc tggcacgcat tgaagagaaa tacctggggc atggcgatga ttttgattac
    17041 gtcgatacgc gcacattttt acgcgccgtc gatgcggtac tgccgcagtt aaagcccctg
    17101 tttgagaaat acgccgaaga aattgactgg cgtttgctgg ccgctattgc ttatcaggaa
    17161 tcgcactggg atgcacaggc cacttcaccg acgggtgtgc gcggcatgat gatgttaacc
    17221 aaaaataccg cgcaaagcct cggcattacg gatcgtaccg atgccgaaca gagcatcagc
    17281 ggtggcgtgc gttatttgca ggatatgatg agtaaagtgc cggaaagtgt gccggagaac
    17341 gagcggatct ggtttgccct cgctgcgtac aatatgggct atgcgcatat gctggatgcc
    17401 cgcgccctga cggcaaaaac caaagggaat cctgacagtt gggctgacgt aaaacagcgt
    17461 ctgcctttac ttagccagaa accctattac agcaagctga cttacggcta cgctcgtgga
    17521 catgaagcct acgcttatgt cgaaaatatt cgtaagtatc aaattagcct ggtgggttat
    17581 ctgcaagaga aagagaagca ggctacagaa gcggcgatgc aactggcgca ggattatccg
    17641 gcggtatcgc ctacggagtt gggcaaagag aaatttcctt ttctctcgtt tctttcccag
    17701 tcgtcatcaa actatttgac ccattctccc tctctgctgt tttccaggaa agggagtgaa
    17761 gagaaacaaa attaatccgt cgaggattgc gcttttttct gcgctttaat ttcctggcgg
    17821 cgcatgcgaa agaagtcact gagcaacgcc gcgcactcat ccgccagtat tccttccgta
    17881 atttccactc ggtgattcat acccggatga tgcagcacat ccattaaaga tcccgcagcg
    17941 ccagttttcg cgtcacgcgc accaaagacc acgcgaccaa tgcgactgtg gatcatcgct
    18001 ccggcacaca ttacacatgg ttcaagcgtg acatacaacg tggcgtcgat cagacgataa
    18061 ttttgcatca ccagaccacc ctgccgcagg gccatgattt ctgcatgtgc ggtgggatca
    18121 tggcgaccaa tcgggcggtt ccaaccttcg ccgattaccc gattgttatg cactaatacc
    18181 gcgccgaccg gcacttcccg ctcatcccag gcacgtttcg ccagcgtcag cgcgtgacgc
    18241 atccagtatt cgtggctaaa ttcgacttca gacaaaaaga aaactccggt tataaaagcg
    18301 cggcgcatta tacacggacg ctatgcttta ctctattcca gttgctggag ttcaccgcgc
    18361 ggggttactc gccaacgatg ctgacagaaa taaagcagcg gattgtcctg attactgtcg
    18421 ctatagccac tgtacagccg cagcggagtg ccgattttgc gctccagttg tgcgaccttt
    18481 tcatgtccca gacaacgcat cgtcaatacc caaccaccat agccacgctg aatttggctg
    18541 gcgataagat taacccgcgg cagccagggc ttatcgaaat aaaccgcttc aaccagcggc
    18601 tgcggagagc cggtaatcaa ccagatatca gcatcggaac ttaacaggta ggtggttaat
    18661 cgctcctgaa ccagcggaaa ggcggtaaca ttgtcgcgaa accagcgcac gaaatcggcc
    18721 tgcaacgtct gtaaacgtgc ttcgctgtga ccaaaagtgc acccccacag aagcagactc
    18781 atcggccagc gtgccgcacg accttttatc aataacgcaa tggctataat cggtaacaac
    18841 ggcaggacaa gtaacgcatt cagcggttgg cgacgtagta aatagcgcag aaaactgccg
    18901 aacatatcct gctgatgcaa tgttccatct aagtcaaaaa acaccacacg acgctcgtga
    18961 gttgccaaac catactcctc tggactgaaa catcctgatt aattttcgct actacatagc
    19021 ctaacagata gatcatcact tttccggcaa caatcgaccg catcatggct aactggaatt
    19081 tttaattcat ggcaattagc ggcaatggaa tataaaattc actcgcgtgt gtctcatatt
    19141 gcctgttgtc gccactattt catcgcgagg aaagatatga acggcttact tcgtatccgt
    19201 cagcgttacc aggggcttgc ccagagtgat aaaaaactgg cggattatct gctgctacaa
    19261 cctgatacgg cgcgccattt aagctctcag caactggcca acgaagccgg agtcagtcag
    19321 tccagcgtcg tgaagttcgc gcaaaaactc ggctataaag gttttccggc gcttaagttg
    19381 gcgttgagtg aagcgctggc aagccagccg gaatcgccct ccgtgcccat tcataaccaa
    19441 atccgcggtg atgatccgtt acggctggtc ggcgaaaaac tgattaaaga aaatactgcc
    19501 gctatgtacg caacgctaaa cgttaatagt gaagagaaac tgcatgaatg cgtaacaatg
    19561 ttgcgctctg cgcggcggat aattctgacc ggtattggcg cttcgggtct ggtggcgcaa
    19621 aactttgcct ggaagctgat gaagattggc ttcaatgctg ccgcagtgcg cgatatgcat
    19681 gcgctactcg caacagtaca ggcgtcgtcc cctgacgatc tgttattagc catttcctac
    19741 accggtgtac gacgcgagtt aaacctggcg gcagatgaga tgctgcgagt gggcggaaaa
    19801 gtgctggcga ttaccggctt tactccgaat gccctgcaac agcgtgcttc tcattgcctg
    19861 tataccattg ccgaagaaca agcgacaaac agtgcttcaa tctctgcttg tcacgctcag
    19921 ggaatgttaa cggatttgct gttcattgcg ctgattcagc aggatctgga actggcacca
    19981 gaacgtattc gtcatagtga agcgctggtg aaaaaactgg tctgagtaaa gaatgcgcgt
    20041 ataatgcccg cccggtttgt gttgttttga gagtttcctt atggcgttgt taatcactaa
    20101 aaaatgcatc aattgtgata tgtgtgaacc cgaatgcccg aatgaggcga tttcaatggg
    20161 agatcatatc tacgagatta acagcgataa gtgtaccgaa tgcgtagggc actacgagac
    20221 accaacctgc cagaaggtgt gcccgatccc caatactatt gtgaaagatc cggcgcatgt
    20281 cgagacagaa gaacagttgt gggataaatt tgtgctgatg caccacgcgg ataaaattta
    20341 atattctact ctggaagtag agtattaatt atattactgg gaagccttaa cgccattata
    20401 tttatttaat tgatgacatt agcataatca ttcactaagt taatttatat agtatctgcc
    20461 aagacactta tttatagtta ttaaaggcgc gtccgattgg ttcaccggac gcaccttaag
    20521 tacgtttcct tgtgttataa gaacagaagg atcagctgta aaacagcaat gatgattttg
    20581 atgacccgtt taatcaggta tcggcaatca gtcattcgtt tttccttaaa caaggaatgc
    20641 agccgttcag attagccctt acatctcccc aaaactgaac gtgcgagtta ttgagggtgc
    20701 atgctgcact ccacaccaga gctttgacga caccactcgt ttcaatgggg gaattctgtg
    20761 gcatggtgta aagcacagca aaatcttcaa taacgaagcc aattttaatg tacttacgaa
    20821 ttggcggtca cgttaatctt tccatcaata ttgctttctt cgtaaaggct cgagttttta
    20881 tgctaaagat tgcaagttgc ttgtaaaaga taagtacact gatccataat cgctgttgtt
    20941 gagggtgcat gctgcacaaa attaaagtta aaaagtaaaa cccccgttcc ttaccagttc
    21001 gggggtttta ctttttaaag agaacggtat tattttaact ttcaataatt accgtggcac
    21061 aagcatagtg gcgttcatct gccagcgtta catgcatatt tacaacgccg agcttttccg
    21121 ccagttttaa tgcctcaccc catagccgta gccgtggttt gccgagctca tcattgaata
    21181 cttcaaattg attaaacgcc agaccattgc ggatcccggt gccaaacgct tttgctgcgg
    21241 cttctttcac agcaaaacgc ttcgccagaa aacgcaccgg ctggtggtgc gttttccaga
    21301 ttgcccattc gttatcgctt aatacgcggc gtgccaggcg ctcaccggat cgggcgatca
    21361 ctgcttctat gcgagcgatc tctacgatat ccgtgcctaa acctaatatt gccattagcc
    21421 acgcgcttcc agcatcagac gcttcatttc tgccaccgca tctttcagtc cggtcatcac
    21481 tgcacggcca ataatggcat gaccgatatt cagttcatgc atctcaggga tggcggcaat
    21541 ggctttcacg ttgtgatagg tcagaccgtg tccggcgtta actttcagac cgagactagc
    21601 ggcaaaggtc gcggctttgg cgatacgcac cagctcttgc gcctgttcgg cgtcagtttt
    21661 ggcatcagca tagcaaccgg tgtggatctc gataaacggt gcgccaacct ctgccgcagc
    21721 tttgatctgc tcttcatcgg cgtcgataaa gagagaaacc tgaatcccgg catctgtcag
    21781 acgtttgcag gcatcgcgca ttttttcacg ctgccctgca acgtccaggc cgccttcggt
    21841 tgttacttcc tgacgctttt ccggtaccag gcagcaaaaa tgtggcttcg tctcaacggc
    21901 gatcgccagc atctcttcgg tcactgccat ctccagattc attcgggtat ccagcgtctg
    21961 ccgcaggatg cgcacatcgc ggtcagtaat gtggcggcga tcttcacgta aatgcacggt
    22021 aatgccgtcc gctcccgcct gctcggcaat aaacgccgcc tgcaccggat ccgggtaagc
    22081 agtaccgcgt gcgttacgca gcgttgcaat atggtcaatg ttgacgccta acagtaattc
    22141 agccatgaca atcctcatca ttcataatgt gttttcaccg ttcgcttagg cataaactgc
    22201 cggaacagtt ccctgctctt taatggttta ccgccaagat acggcttaag cgccatgcgg
    22261 gtaaagcgtt tcgcggcgcg tagtgtgtct gcgtcaggaa attcccgcgc gtttaacgct
    22321 tttaactgcc ttccggtgaa cgttttattg tcgataacga cgcttgcgat aaaccctttt
    22381 tcttcgcgat aacgatacgt catggtgtca tctaccggct cgccgctacc cgcgcaatag
    22441 gtaaaattga cgccatagcc cagatgcccg agcagtgcca gttcaaagcg gcgcagcgcg
    22501 ggttctggcg taccagtggc acctgcaaga gactgaatgc agtgcaaata atcgaaaaag
    22561 agttcagaga agcgcgtctc gtattccagt acgcgggaga gaagttcgtt gatgtacaga
    22621 ccgctgtaaa gcgtgatacc gcttaatggc agcgccagcg agacagcttc agcactgcgc
    22681 agcgttttga cttcgccacg cccgccgaaa cgtagcaaga gaggggtgaa aggctgtaat
    22741 gcacctttca gagtagagcg tttagagcgt gcgcctttgg caaccagacg cacgcgaccc
    22801 gattcctccg tgaagacgtc cagcatcagg ctggtttcgc tccacgggcg actatgcagg
    22861 acaaatgcgc gctgccagcc ttccatcgga gtgactctta aagatcgtca acgtaaccga
    22921 gactgcgcag tgcgcgttcg tcgtcggccc aaccggattt cacttttacc cacagttcaa
    22981 ggtggacagg cgcttcgaac atttcctgca tgtctttacg cgcttcaatc ccgatggttt
    23041 tgatcttggc ccctttgttg ccaataacca tcttcttctg cccttcacgc tcaacgagaa
    23101 tcaaaccatt gatgtcataa ccaccgcgtt cgttagagac gaaacgttcg atctccacgg
    23161 tcacggagta cggcagttca gcgccgagga aacgcatcag tttttcgcgg atgatttcag
    23221 acgccataaa acgctgtgag cgatcggtga tgtaatcttc cgggaagtga tgggtcgctt
    23281 caggcagatg cttacgcacg attgctgcga tagtgtcgac attcagcccg gtttcagcag
    23341 agatcggcac gatatcgagg aagttcatct gacttgccag gaactgtaag tgcggcagca
    23401 gatcggcttt ttcctgcacg ttatccactt tgttcaccgc gaggatcacc ggcgctttgc
    23461 catcgcgcag tttgttgagc accatttcgt cgtccggcgt ccagcgggtg ccttcaacga
    23521 caaaaatcac cagctcaacg tcgccaatag agctgctcgc cgctttgttc atcaggcggt
    23581 taatagcgcg tttttcttcc atatgcaggc caggggtatc aacgtagatc gcctgatacg
    23641 cgccttcagt atggatcccc acaatgcggt gacgagttgt ttgcgctttg cgggaggtga
    23701 tggagatctt ctgccccagc agtttgttca acaatgtgga tttgccaacg ttcggacgtc
    23761 cgacgatggc aataaatccg cagtaacttt tatcgatgct cattccagct ccagtttttt
    23821 caacgcctgt tcggcggcag cctgctcagc cttacgacgg cttgaacctg tgccaaccac
    23881 cggttcactc aggccgctga cctggcagtg gatagtaaat tcctgatcgt gcgcttcgcc
    23941 acgtacctgg actaccagat aagtcggcag cggcagatgg cgaccctgca aatattcttg
    24001 caagcgcgtt ttcggatctt tttgtttatc gcctgggcta atttcgtcca aacgagtttg
    24061 ataccagttg aggattaatt tctcgacggt ttgaatatca ctgtcgagga atacgccacc
    24121 aattaatgct tcgacggtat cggcgagaat tgactcacga cgaaatccac cgcttttaag
    24181 ttcaccaggc cctaaacgta agcactcgcc taactcaaat tcgcgcgcca gttccgccag
    24241 cgtattgcca cggaccagcg tggcgcgcat ccggctcata tcgccttcat ccacacgagg
    24301 gaaacggtga taaagcgcat tggcgataac gtagctcaga atagagtcgc ctaaaaattc
    24361 taaacgctcg ttatgtttgc tgctggcact acgatgagtt aatgcctgct gcaacagttc
    24421 ctgatgatta aaagtgtagc ccagcttccg ttgaagccga ttaattacga tggggttcat
    24481 gcgataccaa taaataaatg cgtcaacaat tcagcacacg aaacagacct gatatacatg
    24541 gctctgctaa ctgcttcgct gcagtttcag tttaccggta tatggggacc aacgctgttt
    24601 cgtgtgccgt ggcaacctgg aggtgccaac cttaaacttc gggggaatat tctatacaca
    24661 acgacggggg atgtcgttag ccacgggaga tttatctcat aaataattca cgttgtcgcc
    24721 ataacggcga caacgtgaac gaagatgggc tattaatgga tgccgccaat gcgacttaag
    24781 cgcacacctg tcggccattc gccttcttgc ttatcgaaac tcatccagat agccgttgcc
    24841 cgaccgacca gattcgcttc aggcacaaaa ccccagtaac ggctgtccgc gctgttgtcg
    24901 cggttgtcgc ccatcatgaa gtattgtccc ggcggaacaa tccaggttgc cagttgttgc
    24961 cctggctgtt ggtaatacat ccccacctgg tcctgcgcaa tcggcactgt cagaattcgg
    25021 tgcgtcacat cacccaatgt ctctttacgc tcggaaagac gaattccatt ttctttggtt
    25081 tcgtttttcg gcacttcaaa gaatccgctg gtcgcttccc caccattacg gcgtgagaag
    25141 gtctgaacga aatcgctcgg ttccacgttg gagtaggtga ccggtagcgc gttttcacac
    25201 gcctggccgg aactgcatcc cggttgaatc gtcagctctt ttgagaccgg atcgtaagtg
    25261 actttatcgc ccggtaaacc caccgcgcgc ttgatgtaat caagctttgg atcttccgga
    25321 tatttaaaga ccacgatatc gccgcgtttc ggatgaccgg tttcgatcag cgttttctgg
    25381 tagataggat ctttaatgcc ataagcaaac ttctctacca gaataaaatc accgattaac
    25441 agagtcggca tcatcgaacc tgacgggatc tggaacggtt cataaataaa cgaacgtaca
    25501 atcaatacga tagccagcac cggaaaaaca gaagcaccgg tttccagcca gccaggcttc
    25561 ggcgcaacct ttttcagggt tgctttatcc agtgagtcac cggcagccgc ctgcgccgct
    25621 gcctgacgtt cccgccgttt aggtgcgaaa aagaatttat ccacgcacca taaaatgccc
    25681 gtcaccagtg tggcaatcac cagaatcagg gcaaacatat tcgccatgcc aactcctaag
    25741 ggttatttgt tgtctttgcc gacgtgcaga atggcgagga acgcttcctg cggcagctcg
    25801 acgttaccga tctgcttcat gcgtttctta ccttctttct gcttctgcag cagctttttc
    25861 ttacggctga tatcgccgcc ataacattta gccagtacgt ttttacgcag ctgtttcacg
    25921 gtggatcgcg caataatgtg cgtaccaatc gctgcctgaa tggcgatatc aaactgctgg
    25981 cgtgggatca gatctttcat cttctccacc aactcgcgac cgcggttttg cgaattatca
    26041 cggtgggtga tcagcgccaa cgcatcaaca cgttcaccgt tgattaatac gtctacacgt
    26101 accatgtcgg acgcctggaa gcgtttgaag ttgtaatcca gagacgcata accacgcgag
    26161 gtagatttca ggcgatcgaa gaagtcgagc accacttccg ccatcgggat ctcgtacgtc
    26221 agcgccacct gattaccgtg gtaaaccata ttggtctgca cgccgcgttt ttctacgcac
    26281 agcgtaataa cgttgccgag atatgcctgc ggcagcagca tgtgacactc tgcaatcggc
    26341 tcgcgcagtt cgtaaatgtt atttaccgca ggcagcttgg atgggctgtc gacgtagata
    26401 acttcccttg acgtggtttc aacttcatac actacggtcg gcgcagtggt gatcagatcc
    26461 agatcgtatt cacgttccag acgttcctgg atgatctcca tgtgcagcag gccaaggaag
    26521 ccgcagcgga aaccaaagcc cagcgcgctg gagctttccg gctcatagaa cagtgaggca
    26581 tcattcaggc tgagtttacc cagcgcgtca cggaaggctt catagtcgtc ggaacttacc
    26641 gggaacagac cggcgtatac ctgcggtttg actttcttaa agccaggcag cgccttttct
    26701 gccggattac gcgccagcgt taaggtatcg ccgactggag cgccgtggat atctttaatc
    26761 gcacatacca gccagcctac ttcgccacat ttcagttcag tgcggtcaac ctgtttcggc
    26821 gtgaagatgc ccagacggtc ggcgttatag gtctgcccgg tactcatgac tttcactttg
    26881 tcgcccttac gcagggtgcc gtttttaata cggataagtg aaacaacgcc caggtagttg
    26941 tcgaaccatg agtcgataat tagtgcctgc aacgggcctt ccggatcgcc ttccggcggc
    27001 ggaatgtcgc gcaccagacg ttcgagaacg tcctgcacgc caacgccggt tttcgctgaa
    27061 cagcgcaccg cgtcagtggc gtcgatgccg acgatatctt caatttcttc cgccacgcgt
    27121 tcaggatcgg ctgccggcag gtcaatcttg ttcagtactg gcaccacttc gagatccatt
    27181 tccatggcgg tgtagcagtt tgccagggtt tgcgcttcca cgccctgccc ggcgtcgacc
    27241 accagcaatg caccttcaca ggcagccagc gaacgggaaa cttcatagga gaagtctacg
    27301 tggcccgggg tgtcgataaa gttaagctga taggtttcgc cgtcagacgc tttgtagtcc
    27361 agcgtcacgc tttgcgcttt gatggtaatg ccacgctcac gctcaagatc catggaatcg
    27421 agaacctgcg cctccatttc acggtcagac aggccaccgc agatctggat aatacggtca
    27481 gacagcgtcg atttaccgtg gtcaatgtga gctatgatcg aaaagttacg tatattcttc
    27541 ataaagtatg attattgtgc cttaatgccc ggttaaccag gcttttagaa gtcgctgttc
    27601 tgagcttaac gtctgtatta atagaaacgc cgcattctac actacaacat tgaggcgagg
    27661 aaatgttcat accgtatgga ttgtggtatc tggaaacgtc ctcgcatttg ttatgcaaaa
    27721 tgcaacaaag ccagtgaaat cactggctcg cgtcttccga agatgtttca aatcgcacaa
    27781 ggccaggcgg caatgccacg cttaagatga tcggttgcca ttctgcccgg gcagcaaact
    27841 tacgcgagta gccgcgcgca atcaggaacc cgccaatacc gccgaggacc gcaccacata
    27901 atgctgcgac gtcagaagca aagagtagct gaaatagcga agcgatgagg aataatccca
    27961 ccagcggcga catataaacc agtaatgcgg agctaagcag gctgccttcg gcgatcccca
    28021 attccacttt ttgccccggc actaacggtt catcacaggg tacgacaatg gtatgcgtgg
    28081 tttgcgggcc aagtttattc aacacgcggc taccgcaacc ggcgcgtgaa gcgcagctgc
    28141 tgcatgaagc tttaacatca caactgacca gcgcctgccc gttttgccag gagacgacgg
    28201 tagcccactc tttgatcatt gcgctgcccc gaacttaata ttctcggcaa tgcgtttcgc
    28261 cgtttgcggc ggcagttcac cgacaatggt gatttcggcg ttatcacgta cgcttgtact
    28321 gacggttctg cgtccggtgc gcaacatctg atcggtgctc gatggcgtag cgcggttaac
    28381 gtttaccgag aagctgaata atccgtcaga atagagacgt gattcgatag gcatgttgtc
    28441 catcgtcggt agcggacgtc gactactgga aacttcgcta aaaccctgtg gcaaccaggt
    28501 tggcgtccag ctgaatttag ctttttcacc tacaggaaca gaaagcaacg gcggcaaatt
    28561 tgccttcgcc agcgtctgca tactgctgct gatatcctga ttgacgttaa aagcaatcac
    28621 gcgaaattgt tccagcgttt caccatcgcg atcaaggaga tcaacccgca tcggtaattt
    28681 cgattcggtg tccatccaca cgatgtagct gtagcgtgta ccatctcggg caaccacgcg
    28741 aatgacttcg caaagacgat cagcaatacg cgtgcgcccg acggagataa agtcgtagta
    28801 aggagaaagg cgtttgaaat cggtatagat gagcgatggc agagaatcaa caatgtaatc
    28861 gccattaagc gtgaacggtt caagtcccgg ttcaaaatag ctgatttcat cgccgcgctg
    28921 taccacttcc cggcgcgggc catccatttg caacaattgt gcaagaggac ggttatcgag
    28981 gcgtgcatgt cgataacgca gagactcaac accctgttta ttgatgctga tgaatgacag
    29041 ctcgtaattc agtgactgac tggccaggtt catctgctgt aataacgccc cggacgcggg
    29101 agtggccgag gcgttagcag agaataacag gctacctgtc actaatgaca tggcaaacca
    29161 aagttgcttc attactgcga ttgcgttcct aaagtttgaa ttcctggcac ctgtacagcg
    29221 gcttgctggg tttgcgcctg ctcaaactga agctgttcag agtggagtcg gcgttgcagt
    29281 tcgtaatcct gcaacattgc attaatgcga cgacgctgct cctgtacctg ctgctgttga
    29341 ccattgtttg cggtcgcttc agaaggtact cccaggctta ccgggctggc tttacccatc
    29401 atcggcagtg tattaaatac cggcgtttcg ggctgctggg acgtttcaga ttgtccatta
    29461 tagtgctgga cgccaacgat aactgcaagc gatacgcatg ctgctacgcc catttgggta
    29521 agctgtgccg cccacggacg tactttctgc cagaatggca ttttctgcca ttgatgcggc
    29581 gcaggctggg cttccgggat caatgtcgcc ggttgacgta ctggctcttc ttcaatggcg
    29641 gccatcacgc gtgaagagat atcgaaatgg agcacctcgg gagtatcacc ccgcattgag
    29701 tcacggatta agtgatagct ttcccaggtt ttctgcattt ctgggttatg agccagttcg
    29761 ttaagcagct cactatccag cgtttcgcca tccattaaag cggaaagttg ttctttctgc
    29821 atgcctaata cccttatcca gtatcccgct atcgtcaacg cctgataagc ggttgaactt
    29881 tgttatcaat agcttccctc gctcggaaga tacgtgaacg caccgtacct accggacaat
    29941 ccatgatagc ggctatctct tcatagctca ggccatccag ctcccgcaag gttattgcca
    30001 tgcgcaaatc ttccgggagg gactcaatag ttcggaaaac tatctgtctc agttcttctg
    30061 acaacattaa gttctcaggg ttcgaaattt ctttcaacgc gccgccactt tcgaagtttt
    30121 cagcttcaat ggcatccaca tcactggaag gtggacgacg cccctgagca accaggtaat
    30181 ttttcgctgt atttacagca atccgataca gccatgtata aaaagcgcta tctccccgga
    30241 acgaatccag cgcacgatag gctttaataa aagcttcttg taccacatcg ggaacatcac
    30301 ccgacggcac atagcgggaa accagactcg ccactttatg ctgatagcgc actaccagta
    30361 agttaaaggc tttctgatct cccttctgga cccgttcaac caggacctgg tccgttaact
    30421 gctcgctcat ccgaggtaaa gtctccccaa accaaatttc cacgcgctat cgaaacgcca
    30481 ctccattagc tgcaatttga gcaagcaaag ggttagagtg tctcgttttt gtaaagttcc
    30541 gtaacgcatc tgtttttgtt tgtcatgctg tagacggatc attatctatc attataagtc
    30601 tacagaatct gaacatcgca ttatctgtgt agaaatgccc atttaactgc ctgaagagta
    30661 acccaacggc ctttttattt caccacctaa tcctccacca gccagtaact tctctttttc
    30721 tcgccgccct gcatcagcgt gtttagcaac tgtaacaaat attaaaatag caggtgttta
    30781 ttcgcacaac atgatgctat gctgaccaaa ccgtgtttag taaattaaac aaagaaaatg
    30841 aatactctcc ctgaacattc atgtgacgtg ttgattattg gtagcggcgc agccggactt
    30901 tcactggcgc tacgcctggc tgaccagcat caggtcatcg ttctaagtaa aggcccggta
    30961 acggaaggtt caacatttta tgcccagggc ggtattgccg ccgtgtttga tgaaactgac
    31021 agcattgact cgcatgtgga agacacattg attgccgggg ctggtatttg cgatcgccat
    31081 gcagttgaat ttgtcgccag caatgctcgt tcctgtgtgc aatggttaat tgaccagggg
    31141 gttttgtttg atacccacgt tcaaccgaat ggcgaagaaa gctaccatct gacccgtgaa
    31201 ggaggacata gtcaccgtcg tattcttcat gccgccgacg ccaccggtag agaagtacaa
    31261 tccacgctgg tgagcaaggc gcagaaccat ccgaatattc gtgtgctgga gcgcagcaac
    31321 gcagttgatc tgattgtctc tgacaaaatt ggcctgccgg gcacgcgacg
//
LOCUS       DADLFA010000028        30860 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000028 DADLFA010000000
VERSION     DADLFA010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30860
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.028"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            248..631
                     /gene="secE"
                     /locus_tag="JQH65_001088"
     CDS             248..631
                     /gene="secE"
                     /locus_tag="JQH65_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAY6138574.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            633..1178
                     /gene="nusG"
                     /locus_tag="JQH65_001089"
     CDS             633..1178
                     /gene="nusG"
                     /locus_tag="JQH65_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAY6138575.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1338..1766
                     /gene="rplK"
                     /locus_tag="JQH65_001090"
     CDS             1338..1766
                     /gene="rplK"
                     /locus_tag="JQH65_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAY6138576.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGAGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1770..2474
                     /gene="rplA"
                     /locus_tag="JQH65_001091"
     CDS             1770..2474
                     /gene="rplA"
                     /locus_tag="JQH65_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAY6138577.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2887..3384
                     /gene="rplJ"
                     /locus_tag="JQH65_001092"
     CDS             2887..3384
                     /gene="rplJ"
                     /locus_tag="JQH65_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAY6138578.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3451..3816
                     /gene="rplL"
                     /locus_tag="JQH65_001093"
     CDS             3451..3816
                     /gene="rplL"
                     /locus_tag="JQH65_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAY6138579.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4136..8164
                     /gene="rpoB"
                     /locus_tag="JQH65_001094"
     CDS             4136..8164
                     /gene="rpoB"
                     /locus_tag="JQH65_001094"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAY6138580.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8241..12464
                     /gene="rpoC"
                     /locus_tag="JQH65_001095"
     CDS             8241..12464
                     /gene="rpoC"
                     /locus_tag="JQH65_001095"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAY6138581.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            12677..13216
                     /locus_tag="JQH65_001096"
     CDS             12677..13216
                     /locus_tag="JQH65_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase growth adaptation protein"
                     /protein_id="HAY6138582.1"
                     /translation="MKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARTHLKRTILALLES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVK
                     FFTIAYNLLRNKQLPQPSK"
     gene            complement(13626..14759)
                     /gene="thiH"
                     /locus_tag="JQH65_001097"
     CDS             complement(13626..14759)
                     /gene="thiH"
                     /locus_tag="JQH65_001097"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HAY6138583.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAVDVERALNASQFTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAKTEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14756..15601)
                     /gene="thiG"
                     /locus_tag="JQH65_001098"
     CDS             complement(14756..15601)
                     /gene="thiG"
                     /locus_tag="JQH65_001098"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HAY6138584.1"
                     /translation="MSSGRNISCRMATRSCFFRLLQGVEMLRIADKTFDSHLFTGTGK
                     FASSQLMVEAIRASGSQLVTLAMKRVDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEE
                     AIFAAHLAREALGTNWLKLEIHPDARWLLPDPIETLKAAEMLVQQGFVVLPYCGADPV
                     LCKRLEEVGCAAVMPLGAPIGSNQGLETRAMLEIIIQQATVPVVVDAGIGVPSHAAQA
                     LEMGADAVLVNTAIAVADDPVNMAKAFRLAVEAGLLARQSGPGSRSHFAHATSPLTGF
                     LEASA"
     gene            complement(15528..15728)
                     /gene="thiS"
                     /locus_tag="JQH65_001099"
     CDS             complement(15528..15728)
                     /gene="thiS"
                     /locus_tag="JQH65_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HAY6138585.1"
                     /translation="MQILFNDQPMQCATGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHILQDGDQILLFQVIAGG"
     gene            complement(15712..16467)
                     /gene="thiF"
                     /locus_tag="JQH65_001100"
     CDS             complement(15712..16467)
                     /gene="thiF"
                     /locus_tag="JQH65_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HAY6138586.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLSRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVVLNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16460..17095)
                     /gene="thiE"
                     /locus_tag="JQH65_001101"
     CDS             complement(16460..17095)
                     /gene="thiE"
                     /locus_tag="JQH65_001101"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HAY6138587.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVISAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17095..18990)
                     /gene="thiC"
                     /locus_tag="JQH65_001102"
     CDS             complement(17095..18990)
                     /gene="thiC"
                     /locus_tag="JQH65_001102"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HAY6138588.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYLTGTHSGVRV
                     PMREIQLSPTLIGGSKEQPQFEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19071..19171)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19224..19700)
                     /gene="rsd"
                     /locus_tag="JQH65_001103"
     CDS             complement(19224..19700)
                     /gene="rsd"
                     /locus_tag="JQH65_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HAY6138589.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQKVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19795..20568
                     /gene="nudC"
                     /locus_tag="JQH65_001104"
     CDS             19795..20568
                     /gene="nudC"
                     /locus_tag="JQH65_001104"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HAY6138590.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVVLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20608..21672
                     /gene="hemE"
                     /locus_tag="JQH65_001105"
     CDS             20608..21672
                     /gene="hemE"
                     /locus_tag="JQH65_001105"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HAY6138591.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKVTRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21682..22353
                     /gene="nfi"
                     /locus_tag="JQH65_001106"
     CDS             21682..22353
                     /gene="nfi"
                     /locus_tag="JQH65_001106"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HAY6138592.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22396..22986
                     /locus_tag="JQH65_001107"
     CDS             22396..22986
                     /locus_tag="JQH65_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HAY6138593.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVVLSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQVGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            23173..23445
                     /gene="hupA"
                     /locus_tag="JQH65_001108"
     CDS             23173..23445
                     /gene="hupA"
                     /locus_tag="JQH65_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HAY6138594.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23458..24153
                     /locus_tag="JQH65_001109"
     CDS             23458..24153
                     /locus_tag="JQH65_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HAY6138595.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALLLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24155..24580)
                     /gene="zraP"
                     /locus_tag="JQH65_001110"
     CDS             complement(24155..24580)
                     /gene="zraP"
                     /locus_tag="JQH65_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HAY6138596.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24818..26194
                     /gene="zraS"
                     /locus_tag="JQH65_001111"
     CDS             24818..26194
                     /gene="zraS"
                     /locus_tag="JQH65_001111"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HAY6138597.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIVQHGVISVTVSESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            26191..27515
                     /gene="zraR"
                     /locus_tag="JQH65_001112"
                     /pseudo
     CDS             26191..27515
                     /gene="zraR"
                     /locus_tag="JQH65_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
     gene            complement(27512..28801)
                     /gene="purD"
                     /locus_tag="JQH65_001113"
     CDS             complement(27512..28801)
                     /gene="purD"
                     /locus_tag="JQH65_001113"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAY6138598.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDDVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACESKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLLLEEVAGGKVFHAGTKLADDEQVVTNGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28813..30402)
                     /gene="purH"
                     /locus_tag="JQH65_001114"
     CDS             complement(28813..30402)
                     /gene="purH"
                     /locus_tag="JQH65_001114"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAY6138599.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7313 a         7968 c         8418 g         7161 t
ORIGIN      
        1 gcaaaagttc tgagctaatt gccgataaca tttgactcaa tgcgcactaa aagggcatca
       61 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      121 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      181 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg
      241 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      301 gtgggtcgtt gtggtggcat tgctcttggt ggcgattgtc ggcaactacc tttatcgcga
      361 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      421 cgcgctgtta acgacaaaag gtaaagctac cgttgctttt gcccgtgaag cgcgtaccga
      481 agtccgtaag gtcatttggc cgactcgcca ggaaacattg cacaccacgc tgattgtggc
      541 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      601 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
      661 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata
      721 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
      781 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
      841 tcgttcagat ggtgatgaac gacgcgagct ggcacctggt gcgcagcgta ccgcgagtga
      901 tgggcttcat cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg
      961 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1021 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1081 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1141 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1201 atttaatcgt tgcacaaggc gcgagattgg aatacaattt cgcgcctttt atttttatgg
     1261 gccttgcccg taaaaacgat tttttatatc acggggagcc tctcagaggc gttattaccc
     1321 aacttgagga atttataatg gctaagaaag tacaagccta tgtcaagctg caggttgcag
     1381 ctggtatggc taacccgagt ccgccagtag gtccggctct gggtcagcag ggcgtaaaca
     1441 tcatggaatt ctgcaaagcg ttcaacgcaa aaactgattc catcgaaaaa ggtctgccga
     1501 ttccggtagt aatcaccgtt tacgctgacc gttctttcac tttcgttacc aagaccccgc
     1561 cggcagcagt tctgctgaaa aaagcggctg gtatcaagtc tggtgccggt aagccgaaca
     1621 aagacaaagt gggtaaaatt tcccgcgctc agctgcagga aatcgcgcag accaaagctg
     1681 ccgacatgac tggtgccgac attgaagcga tgactcgctc catcgaaggt actgcacgtt
     1741 ccatgggcct ggtagtggag gactaagaaa tggctaaact gaccaagcgc atgcgtgtta
     1801 tccgcgagaa agttgatgca accaaacagt acgacatcaa cgaagctatc gcactgctga
     1861 aagagctggc gactgctaaa ttcgtagaaa gcgtggacgt agctgttaac ctcggcatcg
     1921 acgctcgtaa atctgaccag aacgtacgtg gtgcaactgt actgccgcac ggtactggcc
     1981 gttccgttcg cgtagccgta tttacccaag gtgcaaacgc tgaagctgct aaagctgcag
     2041 gcgcagaact ggtaggtatg gaagatctgg ctgaccagat caagaaaggc gaaatgaact
     2101 ttgacgttgt tattgcttct ccggatgcaa tgcgcgttgt tggccagctg ggccaggttc
     2161 tgggtccgcg cggcctgatg ccaaacccga aagtgggtac tgtaacaccg aacgttgctg
     2221 aagcggttaa aaacgctaaa gctggccagg ttcgttaccg taacgacaaa aacggcatca
     2281 tccacaccac catcggtaaa gtggactttg acgctgacaa actgaaagaa aacctggaag
     2341 ctctgctggt tgcgctgaaa aaagcaaaac cgactcaggc gaaaggcgtg tacatcaaga
     2401 aagttagcat ctccaccacc atgggtgcag gtgttgcagt tgaccaggct ggcctgagcg
     2461 cttctgtaaa ctaatgcctt tacgtgggcg gtgattttgt ctacaatctt acccccacgt
     2521 ataatgctta atgcagacgt atatccgaga tattcgggtt gtggcaaggc ggcaactgag
     2581 tgagtcgcca agagcatagc taactatgtg actggtgcga atgaaggaag ccaacgccgc
     2641 cacaagttga atagcgacgg atagaaaaga tttgttcgtt ggagcttggc ctatccaggc
     2701 ctccgtcgaa gaccgcagga gtttcgcaag aaacttaatc ccctgcgtag acggtgacag
     2761 aacgctaaga ttattctttt atattctggc ttgtttctgc tcaccgtaat taagacgctc
     2821 tctccgtttg gaggagtgaa gtgagttcca gagattttct ctggcaaaca tccaggagca
     2881 aagctaatgg ctttaaatct tcaagacaaa caagcgattg ttgctgaagt cagcgaagta
     2941 gccaaaggcg cgctgtctgc agtagttgcg gattcccgtg gcgtaactgt agataaaatg
     3001 actgaactgc gtaaagcagg tcgcgaagct ggcgtataca tgcgtgttgt tcgtaacacc
     3061 ctgctgcgcc gtgctgttga aggtactccg ttcgagtgcc tgaaagacgc gtttgttggt
     3121 ccgaccctga ttgcatactc tatggaacac ccgggcgctg ctgctcgtct gttcaaagag
     3181 ttcgcgaaag cgaatgcaaa atttgaggtc aaagccgctg cctttgaagg tgagctgatc
     3241 ccggcgtctc agatcgaccg cctggcaact ctgccgacct acgaagaagc aattgcacgc
     3301 ctgatggcaa ccatgaaaga agcttcggct ggcaaactgg ttcgtactct ggctgctgta
     3361 cgcgatgcga aagaagctgc ttaatcgcag ttatcttttt aacgcattcg cttacgtata
     3421 aacttattct gatattcagg aacaatttaa atgtctatca ctaaagatca aatcattgaa
     3481 gcagttgcag ctatgtctgt aatggacgtt gtagaactga tctctgcaat ggaagaaaaa
     3541 ttcggtgttt ccgctgctgc tgctgtagct gtagctgctg gcccggttga agctgctgaa
     3601 gaaaaaactg aattcgacgt aattctgaaa gctgctggcg ctaacaaagt tgctgttatc
     3661 aaagcagtac gtggcgcaac tggcctgggt ctgaaagaag ctaaagacct ggtagaatct
     3721 gcaccggctg ctctgaaaga aggcgtgagc aaagacgacg cagaagcact gaaaaaagct
     3781 ctggaagaag ctggcgctga agttgaagtt aaataagcca acccttccgg ttgcagcctg
     3841 agaaatcagg ctgatggctg gtgacttttt agtcaccagc ctttttgcgc tgtaaggcgc
     3901 cagtagcgtt tcacactgtt tgactactgc tgtgcctttc aatgcttgtt tctatcgacg
     3961 acttaatata ctgcgacagg acgtccgttc tgtgtaaatc gcaatgaaat ggtttaagcg
     4021 tgatagcaac aggcattgcg gaaagtgttc cattttccgg tcaacaaaat agtgttgcac
     4081 aaactgtccg ctcaatggac agatgggtcg acttgtcagc gagctgagga accctatggt
     4141 ttactcctat accgagaaaa aacgtattcg taaggatttt ggtaaacgtc cacaagttct
     4201 ggatgtacct tatctccttt ctatccagct tgactcgttt cagaaattta tcgagcaaga
     4261 tcctgaaggg cagtatggtc tggaagcagc tttccgttcc gtattcccga ttcagagcta
     4321 cagcggtaat tccgagctgc aatacgtcag ctaccgcctt ggcgaaccgg tgtttgacgt
     4381 ccaggaatgt caaatccgtg gcgtgaccta ttccgcaccg ctgcgcgtta aactgcgtct
     4441 ggtgatctat gagcgcgaag cgccggaagg caccgtaaaa gacattaaag aacaagaagt
     4501 ctacatgggc gaaattccgc tcatgacaga caacggtacc tttgttatca acggtactga
     4561 gcgtgttatc gtttcccagc tgcaccgtag tccgggcgtc ttctttgact ccgacaaagg
     4621 taaaacccac tcttcgggta aagtgctgta taacgcgcgc atcatccctt accgtggttc
     4681 ctggctggac ttcgaattcg atccgaagga caacctgttc gtacgtatcg accgtcgccg
     4741 taaactgcct gcgaccatca ttctgcgcgc cctgaactac accacagagc agatcctcga
     4801 cctgttcttt gaaaaagtta tctttgaaat ccgtgataac aagctgcaga tggaactggt
     4861 gccggaacgc ctgcgtggtg aaaccgcatc ttttgacatc gaagctaacg gtaaagtgta
     4921 cgtagaaaaa ggccgccgta tcactgcgcg ccacattcgc cagctggaaa aagacgacgt
     4981 caaacttatc gaagtcccgg ttgagtacat cgcaggtaaa gtggttgcta aagactatat
     5041 tgatgagtct accggcgagc tgatctgcgc agcgaacatg gagctgagcc tggatctgct
     5101 ggctaagctg agccagtctg gtcacaagcg tatcgaaacg ctgttcacca acgatctgga
     5161 tcacggccca tatatctctg aaaccttacg tgtcgaccca actaacgacc gtctgagcgc
     5221 actggtagaa atctaccgca tgatgcgccc tggcgagccg ccgactcgtg aagcagctga
     5281 aagcctgttc gagaacctgt tcttctccga agaccgttat gacttgtctg cggttggtcg
     5341 tatgaagttc aaccgttctc tgctgcgcga agaaatcgaa ggttccggta tcctgagcaa
     5401 agacgacatc attgatgtta tgaaaaagct catcgatatc cgtaacggta aaggcgaagt
     5461 cgatgatatc gaccacctcg gcaaccgtcg tatccgttcc gttggcgaaa tggcggaaaa
     5521 ccagttccgc gttggcttgg tacgtgtaga gcgtgcggtg aaagagcgtc tgtctctggg
     5581 cgatctggat accctgatgc ctcaggatat gatcaacgcc aagccgattt ccgcagcagt
     5641 gaaagagttc ttcggttcca gccagctgtc tcagtttatg gaccagaaca acccgctgtc
     5701 tgagattacg cacaaacgtc gtatctccgc actcggccca ggcggtctga cccgtgaacg
     5761 tgcaggcttc gaagttcgag acgtacaccc gactcactac ggtcgcgtat gtccaatcga
     5821 aacccctgaa ggtccgaaca tcggtctgat caactctctg tccgtgtacg cacagactaa
     5881 cgaatacggc ttccttgaga ctccgtatcg taaagtgacc gacggtgttg taactgacga
     5941 aattcactac ctgtctgcta tcgaagaagg caactacgtt atcgcccagg cgaactccaa
     6001 cctggatgaa gaaggccact tcgtagaaga cctggtaact tgccgtagca aaggcgaatc
     6061 cagcttgttc agccgcgacc aggttgacta catggacgta tccacccagc aggtggtatc
     6121 cgtcggtgcg tccctgatcc cgttcctgga acacgatgac gccaaccgtg cattgatggg
     6181 tgcgaacatg caacgtcagg ccgttccgac tctgcgcgct gataagccgc tggttggtac
     6241 tggtatggaa cgtgctgttg ccgttgactc cggtgtaact gcggtagcta aacgtggtgg
     6301 tgtcgttcag tacgtggatg cttcccgtat cgttatcaaa gttaacgaag acgagatgta
     6361 tccgggtgaa gcaggtatcg acatctacaa cctgaccaaa tacacccgtt ctaaccagaa
     6421 cacctgtatc aaccagatgc cgtgtgtgtc tctgggtgaa ccggttgaac gtggcgacgt
     6481 gctggcagac ggtccgtcca ccgacctcgg tgaactggcg cttggtcaga acatgcgcgt
     6541 agcgttcatg ccgtggaatg gttacaactt cgaagactcc atcctcgtat ccgagcgtgt
     6601 tgttcaggaa gaccgtttca ccaccatcca cattcaggaa ctggcgtgtg tgtcccgtga
     6661 caccaagctg gggccggaag agatcaccgc tgacatcccg aacgtgggtg aagctgcgct
     6721 ctccaaacta gatgaatccg gtatcgttta cattggtgcg gaagtgaccg gtggcgacat
     6781 tctggttggt aaggtaacgc cgaaaggtga aactcagctg accccagaag aaaaactgct
     6841 gcgtgcgatc ttcggtgaga aagcctctga cgttaaagac tcttctctgc gcgtaccaaa
     6901 cggtgtatcc ggtacggtta tcgacgttca ggtctttact cgcgatggcg tagaaaaaga
     6961 caaacgtgcg ctggaaatcg aagaaatgca gctcaaacag gcgaagaaag acctgtctga
     7021 agaactgcag atcctcgaag cgggtctgtt cagccgtatc cgtgctgtgc tggtagccgg
     7081 tggcgttgaa gctgagaagc tcgacaaact gccgcgcgat cgctggctgg agctgggcct
     7141 gacagacgaa gagaaacaaa atcagctgga acagctggct gagcagtatg acgaactgaa
     7201 acacgagttc gagaagaaac tcgaagcgaa acgccgcaaa atcacccagg gcgacgatct
     7261 ggcaccgggc gtgctgaaga ttgttaaggt atatctggcg gttaaacgcc gtatccagcc
     7321 tggtgacaag atggcaggtc gtcacggtaa caagggtgta atttctaaga tcaacccgat
     7381 cgaagatatg ccttacgatg aaaacggtac gccggtagac atcgtactga acccgctggg
     7441 cgtaccgtct cgtatgaaca tcggtcagat cctcgaaacc cacctgggta tggctgcgaa
     7501 aggtatcggc gacaagatca acgccatgct gaaacagcag caagaagtcg cgaaactgcg
     7561 cgaattcatc cagcgtgcgt acgatctggg cgctgacgtt cgtcagaaag ttgacctgag
     7621 taccttcagc gatgaagaag ttatgcgtct ggctgaaaac ctgcgcaaag gtatgccaat
     7681 cgcaacgccg gtgttcgacg gtgcgaaaga agcagaaatt aaagagctgc tgaaacttgg
     7741 cgacctgccg acttccggtc agatccgcct gtacgatggt cgcactggtg aacagttcga
     7801 gcgtccggta accgttggtt acatgtacat gctgaaactg aaccacctgg tcgacgacaa
     7861 gatgcacgcg cgttccaccg gttcttacag cctggttact cagcagccgc tgggtggtaa
     7921 ggcacagttc ggtggtcagc gtttcgggga gatggaagtg tgggcgctgg aagcatacgg
     7981 cgcagcatac accctgcagg aaatgctcac cgttaagtct gatgacgtga acggtcgtac
     8041 caagatgtat aaaaacatcg tggacggcaa ccatcagatg gagccgggca tgccagaatc
     8101 cttcaacgta ttgttgaaag agattcgttc gctgggtatc aacatcgaac tggaagacga
     8161 gtaattctcg ctcaaacagg tcactgctgt cgggttaaaa cccggcagcg gattgtgcta
     8221 actccgacgg gagcaaatcc gtgaaagatt tattaaagtt tctgaaagcg cagactaaaa
     8281 ccgaagagtt tgatgcgatc aaaattgctc tggcttcgcc agacatgatc cgttcatggt
     8341 ctttcggtga agttaaaaag ccggaaacca tcaactaccg tacgttcaaa ccagaacgtg
     8401 acggcctttt ctgcgcccgt atctttgggc cggtaaaaga ttacgagtgc ctgtgcggta
     8461 agtacaagcg cctgaaacac cgtggcgtca tctgtgagaa gtgcggcgtt gaagtgaccc
     8521 agactaaagt acgccgtgag cgtatgggcc acatcgaact ggcttccccg actgcgcaca
     8581 tctggttcct gaaatcgctg ccgtcccgta tcggtctgct gctcgatatg ccgctgcgcg
     8641 atatcgaacg cgtactgtac tttgaatcct atgtggttat cgaaggcggt atgaccaacc
     8701 tggaacgtca gcagatcctg actgaagagc agtatctgga cgcgctggaa gagttcggtg
     8761 acgaattcga cgcgaagatg ggtgcggaag caatccaggc tctgctgaag agcatggatc
     8821 tggagcaaga gtgcgaacag ctgcgcgaag agctgaacga aaccaactcc gaaaccaagc
     8881 gtaaaaagct gaccaagcgt atcaaactgc tggaagcgtt cgttcagtct ggtaacaaac
     8941 cagagtggat gatcctgacc gttctgccgg tactgccgcc agatctgcgt ccgctggttc
     9001 cgctggatgg tggtcgtttc gcgacttctg acctgaacga tctgtatcgt cgcgtcatta
     9061 accgtaacaa ccgtctgaaa cgtctgctgg atctggctgc gccggacatc atcgtacgta
     9121 acgaaaaacg tatgctgcag gaagcggtag acgccctgct ggataacggt cgtcgcggtc
     9181 gtgcgatcac cggttctaac aagcgtcctc tgaaatcttt ggccgacatg atcaaaggta
     9241 aacagggtcg tttccgtcag aacctgctcg gtaagcgtgt tgactactcc ggtcgttctg
     9301 taatcaccgt aggtccatac ctgcgtctgc atcagtgcgg tctgccgaag aaaatggcgc
     9361 tggagctgtt caaaccgttc atctacggca agctggaact gcgtggtctt gctaccacca
     9421 ttaaagctgc gaagaaaatg gttgagcgcg aagaagctgt cgtttgggat atcctggacg
     9481 aagttatccg cgaacacccg gtactgctga accgtgcacc gactctgcac cgtctgggta
     9541 tccaggcatt tgaaccggta ctgatcgaag gtaaagctat ccagctgcac ccgctggttt
     9601 gtgcggcata taacgccgac ttcgatggtg accagatggc tgttcacgta ccgctgacgc
     9661 tggaagccca gctggaagcg cgtgcgctga tgatgtctac caacaacatc ctgtccccgg
     9721 cgaacggcga accaatcatc gttccgtctc aggacgttgt tctgggtctg tactatatga
     9781 cccgtgactg tgttaacgcc aaaggcgaag gcatggtgct gactggcccg aaagaagcag
     9841 aacgtctgta tcgctctggt ctggcttctc tgcatgcgcg cgttaaagtg cgtatcaccg
     9901 agtatgaaaa agatgcaaac ggtgaattag tagcgaaaac cagcctgaaa gacacgactg
     9961 ttggccgtgc cattctgtgg atgattgtac cgaaaggtct gccttactcc atcgtcaacc
    10021 aggcgctggg taaaaaagca atctccaaaa tgctgaacac ctgctaccgc attctcggtc
    10081 tgaaaccgac cgttattttt gcggaccaga tcatgtacac cggcttcgcc tatgcagcgc
    10141 gttctggtgc atctgttggc atcgatgaca tggtcatccc ggagaagaaa cacgaaatca
    10201 tctccgaggc agaagcagaa gttgctgaaa ttcaggagca gttccagtct ggtctggtaa
    10261 ctgcgggcga acgctacaac aaagttatcg atatctgggc tgcggcgaac gatcgtgtat
    10321 ccaaagcgat gatggataac ctgcaaactg aaaccgtgat taaccgtgac ggtcaggaag
    10381 agaagcaggt ttccttcaac agcatctaca tgatggccga ctccggtgcg cgtggttctg
    10441 cggcacagat tcgtcagctt gctggtatgc gtggtctgat ggcgaagccg gatggctcca
    10501 tcatcgaaac gccaatcacc gcgaacttcc gtgaaggtct gaacgtactc cagtacttca
    10561 tctccaccca cggtgctcgt aaaggtctgg cggataccgc actgaaaact gcaaactccg
    10621 gttacctgac tcgtcgtctg gttgacgtgg cgcaggacct ggtggttacc gaagacgatt
    10681 gtggtaccca tgaaggtatc atgatgactc cggttatcga aggtggtgat gttaaagagc
    10741 cgctgcgcga tcgcgtactg ggtcgtgtaa ctgctgaaga cgttctgaag ccgggtactg
    10801 ctgatatcct cgttccgcgc aacacgctgc tgcacgaaca gtggtgtgac ctgctggaag
    10861 agaactctgt cgacgcggtt aaagtacgtt ctgttgtatc ttgtgacacc gactttggtg
    10921 tatgtgcgca ctgctacggt cgtgacctgg cgcgtggcca catcatcaac aagggtgaag
    10981 caatcggtgt tatcgcggca cagtccatcg gtgaaccggg tacacagctg accatgcgta
    11041 cgttccacat cggtggtgcg gcatctcgtg cggctgctga atccagcatc caggtgaaaa
    11101 acaaaggtag catcaagctc agcaacgtga agtcggttgt gaactccagc ggtaaactgg
    11161 ttatcacttc ccgtaatact gaactgaaac tgatcgacga attcggtcgt accaaagaaa
    11221 gctacaaagt accttacggt gcggtactgg cgaaaggcga tggcgaacag gttgctggcg
    11281 gcgaaaccgt tgcaaactgg gacccgcaca ccatgccggt tatcaccgaa gtaagcggtt
    11341 ttgtacgctt tactgacatg atcgacggtc agaccattac tcgtcagaca gacgaattga
    11401 ccggtctgtc ttcgctggtg gttctggatt ccgcagaacg taccgcaggt ggtaaagatc
    11461 tgcgtccggc actgaaaatc gttgatgctc agggtaacga cgttctgatt ccgggtaccg
    11521 atatgcctgc gcagtacttc ctgccgggta aagcgattgt tcagctggaa gatggcgtac
    11581 agatcagctc tggtgacacc ctggcgcgta ttccgcagga atccggcggt accaaggaca
    11641 tcaccggtgg tctgccgcgc gttgcggacc tgttcgaagc acgtcgtccg aaagagccgg
    11701 caatcctggc tgaaatcagc ggtatcgttt ccttcggtaa agaaaccaaa ggtaaacgtc
    11761 gtcttgttat caccccggta gacggtagcg atccgtacga agagatgatt ccgaaatggc
    11821 gtcagctcaa cgtgttcgaa ggtgaacgtg tagaacgtgg tgacgtaatt tccgacggtc
    11881 cggaagcgcc gcacgacatt ctgcgtctgc gtggtgttca tgctgttact cgttacatcg
    11941 ttaacgaagt acaggacgta taccgtctgc agggcgttaa gattaacgat aaacacatcg
    12001 aagttatcgt tcgtcagatg ctgcgtaaag ctaccatcgt taacgcgggc agctccgact
    12061 tcctggaagg cgaacaggtt gaatactctc gcgtcaagat cgcaaaccgc gaactggaag
    12121 cgaacggcaa agtgggtgca acttactccc gcgatctgct gggtatcacc aaagcgtctc
    12181 tggcaaccga gtccttcatc tccgcggcat cgttccagga gaccactcgc gtgctgaccg
    12241 aagcagccgt tgcgggcaaa cgcgacgaac tgcgcggcct gaaagagaac gttatcgtgg
    12301 gtcgtctgat cccggcaggt accggttacg cgtaccacca ggatcgtatg cgtcgccgtg
    12361 ctgcgggtga agctccggct gcaccgcagg tgactgcaga agacgcatct gccagcctgg
    12421 cagaactgct gaacgcaggt ctgggcggtt ctgataacga gtaatcgtta atccgcaaat
    12481 aacgtaaaaa cccgcttcgg cgggtttttt tatgggggga gtttagggaa agagcatttg
    12541 tcagaatatt taaggaattt ctgaatactc ataatcaatg tagagatgac taatatcctg
    12601 aaactgactg aactaattga gtcaaactcg gcaaggattc gatactattc ctgtgtaact
    12661 ttcttaagga acgagaatga aacaggaagt ggaaaagtgg cgaccttttg gacatccgga
    12721 tggtgatatt cgtgatttat catttcttga tgctcatcag gctgtctacg ttcagcatca
    12781 tgagggcaaa gagcctttag agtatcgctt ttgggttacc tactctcttc actgcttcac
    12841 aaaagattat gaacatcaga cgaacgaaga aaaacaatcg ttaatgtacc acgcgcctaa
    12901 agaatctcgt cccttctgcc agcaccgtta taacttagcg cgcacacact taaaaagaac
    12961 tattttggcg ctgctagaaa gcaacgttat tcatgccggg tatggtagct atgccgtgat
    13021 tgaggtggac ttagacggag gagataaggc attttacttt gttgcgttca gggctttcag
    13081 ggaaaagaaa aaactccgtt tgcatgtaac tagcgcttat cccatttctg aaaaacagaa
    13141 aggtaaatca gtgaaatttt tcaccattgc ctacaactta ttgagaaata agcagcttcc
    13201 tcagccctca aaataacaaa acccacctta aggtgggttt cgccagagaa ttatctctgg
    13261 tattcagaac gccattaccg gactttgcct tgaccttgcg ataatcgcag gttgcgggat
    13321 gtctgaattt cttcagtctg ctgcatcctg gaagatgaga acatgtgttc ttattttcgt
    13381 ctctatcata gttgagtatt tactctctta caatcagatc tctttcattg ctcaacaggc
    13441 gatggcttca gactttgcat tacggaattt ttaagaaagg cagggcgaaa cgaggaagaa
    13501 gcttttcgcc ctggacaaca ttcctgctga cggcactacc ataaaaaaac acaacgcttt
    13561 caacaacctt gcgtttttca tcttccgtag cggctcggaa taaatttacg ttcttacatt
    13621 ccgtctcata gtctttgcga ggcccgtccc agatagctgt cccagtcttt ccataccggc
    13681 tgcaaaccct gagcggttaa cgcggcagca accgcttccg gtctgcgatc gtcgtgcggt
    13741 gagaactgtt ccaactcggg gtgattatcg gcatagccac ctggctgcgt tttcgagaag
    13801 gcgctgacgt tattgatcgc cagcggaata acgcgatcgc gaaaccacgg tgattcccgc
    13861 gtggagagtg acagttcaat ctccggtgca agcagtcgga aggcgcagat ggtttgcact
    13921 aactggcgtt catccataat cgacgcaggc tcaatgccgc cagtacacgg gcgcaggcgc
    13981 ggaaaggaga cagagtaacg gctttgccag taatgctgtt gcagccatag caaatgttcg
    14041 gcaaccatat agcagtcaac gcgccagttg tcggaaaggc caattagcgc gccgaggcct
    14101 atcttatcaa tccccgcacg ccccagccga tccggcgttt ccagccgcca gaagaagtcc
    14161 tgttttttgc ctttcagatg atggcgggca taagtcgcct cgtgatatgt ctcctgataa
    14221 accatcacgc catccagacc aagctgcttt aactcggcgt attccgtctt cgccagcggt
    14281 tgcacttcca tttgtagtga agagaactgt tcacgcaggg cggggagatg acgacgaaag
    14341 taatccatcc ccactttcgc ctgatgttca ccagtgacta atagcagatg ttcaaagccc
    14401 atctcccgta tagcggcgct ttccctggca atatccgctt catccagcgt tttgcgcttg
    14461 atgcgattac tcatggaaaa tccgcagtac gtgcagtcgt tagcgcaaag attggaaaga
    14521 taaagcggga cgtagaaact aactgtgttg ccaaatcgct gacgggtcag acgctgcgcc
    14581 cgttgggcca gttgttccag atagccactg gcggctggcg ataacagcgc catcatatcg
    14641 tcgcgggtga attgcgaggc atttagcgcc cgctctacgt caacagccgt tttgccgttg
    14701 atacgcaggc ggatgtcatc ccagtccagt tgtcgccagc gatcgctgaa ggttttcatg
    14761 ccgatgcctc cagaaatcca gtcagcgggc tggtggcatg agcaaaatga ctgcggctgc
    14821 ccggtccgga ctgacgtgcc agcaggcctg cttctaccgc cagacgaaat gccttcgcca
    14881 tgttgacggg atcgtccgct acggcaatcg ccgtattcac taacaccgcg tcggccccca
    14941 tttccagggc ctgcgctgca tggctgggaa cgccgatgcc agcatcgaca accaccggaa
    15001 ctgtggcctg ctggataata atctccagca tggcacgggt ttccagtccc tgattcgagc
    15061 caatcggcgc gccgagcggc atcaccgctg cacagccgac ttcttccaga cgtttacaca
    15121 ataccggatc ggccccgcag taaggcagca cgacaaatcc ctgttgtacc agcatttcgg
    15181 cggctttcag agtttcgatg ggatcgggca acagccagcg ggcgtcaggg tgaatctcta
    15241 attttaacca gtttgtgcct aacgcttcac gtgccagatg ggcggcgaaa atggcttctt
    15301 ccgctgtttt cgccccggaa gtatttggca gcagggtcac acccgccgcg ataagcggtt
    15361 cgaggatagc gtcgttgtgc tggcgcaagt cgacacgttt catcgccagt gtcaccagct
    15421 ggctgccgga agcgcggatc gcctccacca tcagttgtga agaagcgaat ttccctgtgc
    15481 cggtaaacag atgtgaatca aacgttttgt ccgcaatacg taacatctca accccctgca
    15541 ataacctgaa aaagcaggat ctggtcgcca tcctgcagga tatgttgcgt ccactgctca
    15601 cgcgggacga tttgctgatt aatcgccaga gccgcgcccg cttgtcgttg gtcgagttgc
    15661 tccagtagtt cgtgaacagt ttgcccggtg gcgcactgca tcggttgatc gttaaacagg
    15721 atctgcattg cttcctccgc ataccgggca accactggcg cggcgcaacg ccaggctgcg
    15781 ccactggctc gatttaccgt cgaacagtcg gagttctccc gcaggtgtct ctataccgct
    15841 taataactta atggcttcca gtgcctgcaa agtgcccata accccgacca ccgggccaac
    15901 cacgcccgcc gtgcggcagt tgcgttctgg ctcctggtta tctggccaca ggcagcggta
    15961 acacccctgc tcccagggcg gcgtcagtac catcaactga ccgccaaatc cgaccgcgct
    16021 ggcggtgata agcggcgtgt tgagtaccac gcaggcggca ttaatctcct ggcgagtcgc
    16081 catattgtcg gtacagtcga gcaccacatc ggcccgtgca accgcatctt ttaacgcctc
    16141 acccgttaac cgttgttgta atgctgtcag ttgaatgtcg ggattcaact gtgtcagtcg
    16201 ctgttggctg acctgcgatt tcgggctgtt gatatcttca gtggtaaaga gaatttgtcg
    16261 tgacagattg cttaaatgca catcgtcgtc atctgccagt accagcgtcc cgacgccagc
    16321 gcccgccagg tatagcgcag caggtgtacc cagcccgcca agaccgataa tcagcacctg
    16381 gctgtcgagc agtttttgct gcccgtcaag agcgatatca tcgagcagga tttggcggct
    16441 ataacgcata aaatcacggt cattcatcgc caactcctgc aatttccagc aactgtgccg
    16501 ttgccaaacg ccagtctgcg gcttgagtaa tggcgctgat gacggcgata ctgccgacac
    16561 ccgttgctat caccgcaggc gcgcgtgcca gactgatacc gccaatcgcc acggtgggat
    16621 aatccgccag tcgctcaaca tgccgtgcca gctgttccag cccctgcggt gcagaaggca
    16681 tctgtttggt ttgcgtcggg aacacatgtc ccagcgcgat ataagagggg cgtgctgcca
    16741 gcgcgacgtc gatttccata tcgtcatgtg tcgaaacacc cagccgcagg cctgccgcgc
    16801 ggatggcatt gagatcggtg gcttgcaaat cttcctgccc caaatggacg ccatacgcct
    16861 gatgcttgat cgcgagccgc cagtaatcgt tgataaacaa tcgcgcgtta tagcggcggc
    16921 ccagcgcaat tgccgccacg acatcggctt ccacctcttc atcgcgccga tctttgatgc
    16981 gtagctggag agtacgtacg cctgcatcca acagacgttc gatccactgt acgctgtcca
    17041 ccactgggta cagtcctaaa cgaaaaggta caggaggaaa atcaggctga tacatcacgc
    17101 ttcctcctta cgcaggtaga tttctccgcc tctggcacgg aagttctccg acatatccgc
    17161 cattcccatt tcaatagttt gcgtggcggc gtaatcacgc acttcctggc tgattttcat
    17221 cgagcagaat ttcggcccac acatggagca aaaatgggcg actttacctg actcttgcgg
    17281 cagggtttca tcgtgataag cgcgggcggt aaacgggtcg agggccagat taaactggtc
    17341 ttcccagcga aattcgaagc gggctttcga catggcgtta tcgcgaattt gcgcgcccgg
    17401 atgccctttc gccaggtcag cggcgtgggc ggcaatctta taggtgataa gcccctgctt
    17461 aacatcttct ttattaggca gacccagatg ctcttttggt gttacgtaac agagcatcgc
    17521 gcagccaaac cagccaatca tcgccgcacc aatccccgac gtgaagtggt catagcccgg
    17581 cgcaatatcg gtagttagcg gccccagagt gtaaaacggc gcttcgtggc agtgctctaa
    17641 ctcctcggtc atattgcggc ggatcatctg catcggcacg tggcctgggc cttcaatcat
    17701 cacctgcacg tcatattccc aggcaatttt ggtcagttcg cccagcgtat gcagctcggc
    17761 aaactgcgct tcatcgttgg cgtcctgaat agaaccgggg cgcagaccgt cgcccagcga
    17821 cagcgaaacg tcataagcgg cacagatttc acaaatttcg cggaagtgtt gatagaggaa
    17881 attttcctga tgatgggaga ggcaccattt cgccataatc gaaccgccgc gagagacgat
    17941 accggtcagg cgtttcgcgg tcatcggcac atagcgcaac agtacgcccg catggatagt
    18001 gaagtaatcc acaccttgct cggcctgttc cagcagcgtg tcgcggaacg cttcccaggt
    18061 aagatcttcg gcgatcccgt taaccttctc cagcgcctgg tagatcggca ctgtaccgat
    18121 cggcaccgga ctgttacgca aaatccactc gcgggtttcg tgaatatagc gaccggtgga
    18181 gagatccatc accgtatccg ctccccagcg cgtggaccat accagctttt ccacttcttc
    18241 ttcgatggaa gaggtgaccg ccgagttgcc gatattggcg ttaactttta ccaggaagtt
    18301 acggccaata atcatcggct ccgattccgg atgattaatg ttggccggga taatcgcacg
    18361 tccggcagca acttcatcac ggacaaattc cgcagtgata ttttccggca gacgtgcgcc
    18421 aaagctcatt cccggatgct ggtggcgtaa aacttcgcta cgtatgcgct cgcggcccat
    18481 attctcgcgg atggcgatga attccatttc cggagtgatg atgccctggc gggcgtagtg
    18541 cagttgggtg acacggcgtc ctgctttggc gcgttttggt gttagtacgc cgctaaaacg
    18601 cagttcgtcg aggccatcat ctgccagccg cgctttagtg taatcggaac tgcgcacggt
    18661 aagttcttcg gtatcgccgc gcgcatcgat ccacggctgg cgtagttttg ccagcccttg
    18721 ctgcacgtta atggcgatct gcggatcgcc atatgggccg gaggtgtcgt agaccggaat
    18781 cgcttcgttt tcttcaaact gcggctgttc tttgctgccg ccgattagcg tcgggctaag
    18841 ctggatctca cgcatcggca cgcgaacgcc agagtgtgtg ccagtgaggt aaatgcgttt
    18901 tgagttggga aaggcggtgc cttccagggt gtcgataaaa tgttgggccc gggcgcgttg
    18961 ttcgcggcgg gtcagttttg ttgcagacat agctcattcc aaaaagttaa ggacgtggct
    19021 tgtcagacga cggatgaagc aagagacgat cgccgcaaag gcgatgcgat agcagattaa
    19081 ctcttgttcc cttcgcaggt attagcctga tcaggttccg cggatcccga ataaacggtc
    19141 tcagccagtt aaggcactcc gacaagaaat tagcccccac aaacggggcg ttgaatgtaa
    19201 attacgcgtt aacagcgcag aactcaagca ggatgtttga cgcgggcggc gtcaagcacc
    19261 agcagaatca gcttatcttc cagcacaaag cgcgcttcca gcgcctcgcc gatgtcagat
    19321 aaaacctttt gaaactcaag gtaattatca tgatcgatgg cggtttccag gctggaatcg
    19381 tagtaatcca taatctgttg ggtattggct tcgagttgcg gccaaatctt cgcggcgcgc
    19441 gcgagttgcc cgttgccttc cagcttatga agaatgcgct cataaatact gaaatgtccg
    19501 gcagacaagt aatcgaccag gctctgacaa aaatcatcaa gggctttttc gtttagcctc
    19561 atgtacgatt ctttgccagg cttaatgcca accagattgt agtaagccac gagcagatgc
    19621 ttacgtacat gtagccagcg atcaaccagt ttgttacttc ctctgacgcg ctccgtcagg
    19681 ttatcgagct ggttaagcat gattgactcc gcaagtttgt attcaaaaac tgctcagtga
    19741 gaaatgtaaa aaccatgtta aacatgccag tgatgcaaag gtagtgcaag agctatggat
    19801 cgtataattg aaaaattaga tcacggctgg tgggtcgtca gccatgaaca aaaattatgg
    19861 ttgccgaagg gagaattgcc atatggcgaa gcggcaaatt tcgatcttgt gggtcagcgc
    19921 gcactacaaa tcggcgaatg gcagggggaa cctgtttggt tagtacaaca gcagcggcgt
    19981 cacgatatgg ggtcggtacg tcaggtcatt gatctcgatg ttgtgctgtt tcaactggcc
    20041 ggacgaggcg tacaactggc ggagttttac cgatcgcata aatactgtgg ttactgcggg
    20101 catgaaatgt atccgagcaa aaccgaatgg gcgatgctgt gcagccattg ccgtgagcgt
    20161 tactacccgc aaatcgcccc ctgcattatt gttgccatcc gtcgcgatga ttcgatcctc
    20221 ctcgcccagc atacccgcca tcgtaacggt gtccatacag tacttgccgg attcgtcgaa
    20281 gtgggcgaaa ccctcgagca ggcagtcgcg cgggaagtga tggaagagag cggaattaaa
    20341 gttaaaaact tgcgttacgt gacttctcag ccgtggccgt ttcctcagtc tttaatgacc
    20401 gcgtttatgg cggaatatga cagcggcgac atcgtgatcg acccgaaaga attgctcgag
    20461 gcgaactggt atcgctatga cgatttgccg ttactcccgc cgcccggcac cgtagcgcgc
    20521 cgtctgatag aagatactgt ggcgatgtgt cgggcagagt atgagtgatg atacactgac
    20581 cgcctgacgc actaaggaac agccaaaatg accgaactta aaaacgatcg ttatctgcgg
    20641 gcgctgctgc gccagcccgt tgatgtcact ccagtatgga tgatgcgcca ggcgggtcgc
    20701 tatctaccgg aatataaagt cacgcgcgcc caggcgggcg attttatgtc gctgtgcaaa
    20761 aacgccgagc tggcgtgcga agtgactttg caaccgctgc gtcgctaccc gctggatgcg
    20821 gcgatcctct tttccgatat cctcaccgtg ccggacgcga tggggttagg gctctatttt
    20881 gaagccggag aaggtccgcg ttttacctcg ccagtcacct gcaaagccga cgtcgataag
    20941 ctgccaattc cggacccgga agatgagctg ggttacgtga tgaacgcggt gcgtaccatt
    21001 cgtcgcgaac tgaaaggcga agtgccgctg attggttttt ccggcagccc gtggacgctg
    21061 gcaacctaca tggtggaagg cggcagcagc aaagccttca ccgtgatcaa aaaaatgatg
    21121 tatgccgatc cgcaggcgct gcacgctcta ctcgataaac tggcgaaaag cgtcactttg
    21181 tatctgaatg cgcagattaa agccggtgct caggcagtga tgattttcga cacctggggc
    21241 ggtgtgctta ccgggcgcga ttatcaacag ttctcgctct attacatgca taaaattgtt
    21301 gatggtttac tgcgtgaaaa cgacggtcgc cgcgtaccgg tcacgctgtt taccaaaggc
    21361 ggcggacagt ggctggaagc gatggcagaa accggttgcg atgcgttggg cctcgactgg
    21421 acaacggata tcgccgatgc gcgccgccgt gtgggcaata aagtcgcgtt gcagggtaat
    21481 atggatccgt cgatgctgta cgcgccgcct gcccgcattg aagaagaagt agcgactata
    21541 cttgcaggtt tcggtcacgg cgaaggtcat gtctttaacc ttggtcacgg cattcatcag
    21601 gatgtgccgc cagaacatgc tggcgtgttc gtggaggcag tgcatcgact gtctgaacaa
    21661 tatcaccgct aaggagtgat tatggatctc gcgtcattac gcgctcaaca aatcgaactg
    21721 gcttcttctg tgatccgcga ggatcgactc gataaagatc caccggatct gatcgccgga
    21781 gccgatgtcg ggtttgagca gggcggagaa gtgacgcgag cggcgatggt gctgctgaaa
    21841 tatccctcgc ttgagctggt ggagtataaa gttgcccgca tcgccaccac catgccttac
    21901 attccaggtt ttctttcctt ccgcgaatat cctgcgctgc tggcagcgtg ggagatgctg
    21961 tcgcaaaagc cggatttagt gtttgtcgat ggtcatggga tctcgcatcc tcgccgtctt
    22021 ggcgtcgcca gccattttgg tttgatggtg gatgtgccaa cgatcggcgt ggcgaaaaaa
    22081 cggctctgcg gtaaattcga accgctctcc agcgaaccgg gcgcgctggc cccactgatg
    22141 gataaaggcg agcagctggc ctgggtctgg cgcagcaaag cgcgctgtaa cccgttgttt
    22201 atcgctaccg gccatcgggt cagcgtggac agcgcgctgg cgtgggtaca acgctgcatg
    22261 aaaggctatc gtctgccgga gccaacgcgc tgggcggacg cggtggcctc ggaacgtccg
    22321 gcgttcgtgc gctatacagc aaatcagccc taattcaggt aaactgcgga taatttccgt
    22381 atttgagaac ttaacatgtt acaaaaccca attcatctgc gtctggagcg cctggaaagc
    22441 tggcagcatg tcactttcat ggcttgctta tgcgaacgca tgtaccccaa ttacgccatg
    22501 ttctgccagc aaaccggttt tggtgatggg caaatttacc gtcgtattct cgatctcatc
    22561 tgggaaacgc tgaccgttaa agacgcaaaa gtaaatttcg acagccaact ggagaaattt
    22621 gaagaagcga ttccttcagc cgacgatttc gatctgtacg gcgtttatcc ggcaatcgat
    22681 gcctgcgtgg tgttaagtga actggtccat tcgcgtttga gtggtgaaac gctcgaacac
    22741 gcggtggaag tgagtaagac ctccatcacg accgttgcga tgctggaaat gactcaggtt
    22801 ggtcgcgaaa tgagcgatga agagcttaaa gaaaacccag ctgtagagca agaatgggac
    22861 attcagtggg aaatattccg acttttagct gagtgcgaag aacgcgatat cgagctgata
    22921 aaaggcctta gggcagacct gcgtgaggcg ggtgagagca atattggtat aatttttcag
    22981 caataagacc agaaaacgtg atttaacgcc tgatttgtcg tacttggagt cttccctttc
    23041 gccccccgtc tggtctacat ttggggggcg aaaaaaagtg gctatcggtg cgtgtatgca
    23101 ggagagtgct attctggcat ttccgtcgca ctcgatgctt agcaagcgat aaacacattg
    23161 taaggataac ttatgaacaa gactcaactg attgatgtaa ttgcagagaa agcagaactg
    23221 tccaaaaccc aggctaaagc tgctctggag tccactctgg ctgcaattac tgagtctctg
    23281 aaagaaggcg atgctgtaca actggttggt ttcggtacct tcaaagtgaa ccaccgcgct
    23341 gagcgtactg gccgcaaccc gcagaccggt aaagaaatca aaattgccgc agctaacgta
    23401 ccggcatttg tttctggcaa ggcactgaaa gacgcagtta agtaagattg cgtggcagtg
    23461 aacagtttta acgaaggggt ggtttcaccc cttttgtctt tctggcgtcg atcattgatg
    23521 ctggctggcg ctctgcttct cactgcctgt agtcataact cttcacttcc tccctttacc
    23581 gccagtggat ttgctgaaga ccagggcgcg gtacgcatct ggcgaaaaga cagcggcgat
    23641 aatgtgcatc tgcttgccgt gtttagcccg tggcgcagtg gcgataccac gacgcgagag
    23701 tatcgctggc agggcgataa cctcacgctc atcaatatca atgtttacag caaaccgccg
    23761 gtgaatattc gcgcgcgttt tgacgatcgc ggtgatctga gctttatgca acgtgaatcc
    23821 gatggggaaa agcagcagct ttctaacgac caaatcgatt tataccgtta tcgtgctgat
    23881 cagatccgcc agattagcga tgccttacgt caggggagag tcgtgctgcg ccaggggcgc
    23941 tggcatgcga tggaacagac cgtgaccacc tgcgaagggc aaaccattaa acctgattta
    24001 gattcgcagg cgatagcgca tatcgagcgc cgccagagcc gctcttctgt tgatgtcagc
    24061 gtggcatggc tggaagcgcc cgaaggttcg caattactgt tagtggcaaa ctctgatttc
    24121 tgtcgctggc aacccaacga gaaaacgttc tgatttacca gtggcccata cccatatgac
    24181 cgccaccacc gcagccgccg tagcccatgc ccattccggc tccgcgcgga atacccgctt
    24241 cagccatcgc gatatctcgt ttcacccgta actcatctaa cgactgacgc aaattctcca
    24301 tctctttggc gaccgcgtta attttgctgc tatccggtgg gttcgcggct aacagggcat
    24361 tgtattcata acgcttcgtc accagttgct gttgcagtgc gctgctttga gcgtaaaagt
    24421 cattatggat tttctgccac gctgtctgtt gttcgctggt caaaggcgcg gcattttgct
    24481 gccacatacc gtgtccgccg tgagcaaatg cagatgtcga tcccatcgcc attgctgaaa
    24541 gcgccatcat taccagggca attttcgtgt tccgtttcat ggttaatcct ccagtggttg
    24601 tcttacttcg ggtattgcat cttccgtgcc aacgatgaaa cgctgatatg acgggtaatc
    24661 tggcatgata aacgagtaaa aatgactcgc ctgctgcggg tagcgagtca tttttactca
    24721 ttgaaactta agcctttgtg ttacagcgca gggtaagcgc tgataaaaga tggcatgatt
    24781 tctgctgtca gaaagggatg agcaggcaaa gaagaagatg cgttttatgc aacgttctaa
    24841 agactcctta gctaaatggt taagcgcgat cctccccgtg gtcattgttg ggctggtagg
    24901 gttgtttgcg gtgacggtga ttcgcgatta tgggcgcgag actgccgccg ccagacaaac
    24961 gctgctggaa aaaggcagtg tacttattcg cgctcttgaa tccggctcgc gcgtcggcat
    25021 ggggatgcgc atgcatcatg cgcagcagca ggccttactg gaagaaatgg ccgggcagcc
    25081 tggagtacgt tggtttgcgg tcacggatga acaaggaaca atcgtgatgc atagcaactc
    25141 cggcatggtg ggaaaacagc tttattcccc gcaggaaatg cagcagttac atccgggaga
    25201 tgaagaagcg tggcggcgga tcgatagcgc agacggtgag cctgttctgg aaatttatcg
    25261 ccagtttcaa ccgatgtttg ctgctggaat gcaccggatg cgccatatgc agcagtatgc
    25321 cgcgacacca caagcaattt tcatcgcttt cgacgccagt aatattgtga gtgccgaaga
    25381 tcgtgagcag agaaacaccc tgattatcct cttcgccctg gcgacggtct tgctggcaag
    25441 cgtattgtca ttcttctggt atcgccgcta tctgcgctcg cgccagcttc tacaagatga
    25501 aatgaagcgc aaagagaagc tggtggcact ggggcatctg gcggcaggcg ttgcccacga
    25561 aatccgtaat ccactttcct caattaaagg gctggcgaaa tactttgccg aacgcgcgcc
    25621 agcaggggga gaagcgcatc aactggcgca ggtgatggcg aaagaagccg accgtttaaa
    25681 ccgcgtggta agcgagttgc tggaactggt taagccaacg catctggctt tgcaggcggt
    25741 ggatctcaac acgctgatta accactcatt acagctggta agccaggatg caaacagccg
    25801 ggagatccag ttacgcttta ccgccaacga cacattaccg gaaattcagg ccgatccgga
    25861 caggctgact caggtcctgt tgaatctcta tctcaatgct attcaggcga ttgttcagca
    25921 tggcgtgatt agcgtgacgg tcagcgaaag cggcgcgggc gtgaaaatca gcgttaccga
    25981 cagcggtaag ggaattgcgg cagatcagct tgaagccatc ttcactccgt acttcaccac
    26041 caaagccgaa ggcaccggat tggggctggc ggtcgtgcat aatattgttg aacaacacgg
    26101 tggtacaatt caggtcgcaa gccaggaggg aaaaggctca acgttcaccc tctggcttcc
    26161 ggtcaatatt acgcgtaagg acccacaagg atgacgcacg ataatatcga tattctggtg
    26221 gtggatgatg acattagcca ctgcactatt ttgcaggctt tactgcgcgg ctggggctat
    26281 aacgtcgcgc tggcgaacag cgggcgacag gcgctggagc aggtgcggga acaggttttt
    26341 gatcttgtgc tttgcgatgt gcgaatggcg gagatggacg gcatcgccac gctgaaagag
    26401 atcaaagcgt taaacccggc aattccggtg ctgattatga ctgcgtactc cagcgtcgag
    26461 acggcggtag aggcactgaa aactggggcg ctggattatc tcatcaagcc gctggatttc
    26521 gataacctac aggcgacgct ggaaaaggcg ctcgcgcata cgcacagtat tgatgctgaa
    26581 acacctgcgg tgactgccag ccagttcggt atggtcggta aaagcccggc gatgcaacac
    26641 ctgctcagtg aaatcgccct cgtcgcgcca tcggaagcca cagtgctgat ccacggcgat
    26701 tccggcaccg gtaaagagct ggtcgccagg gcgattcacg ccagtagcgc acgtagcgaa
    26761 aaaccgctgg taacgctcaa ctgtgcggca ctcaacgaat ccttgctgga atctgaattg
    26821 ttcggtcacg aaaaaggggc gtttactgga gccgataaac gccgggaagg gcgctttgtt
    26881 gaggcggacg gcggcacgct gtttctcgat gaaattggcg atatctcgcc gatgatgcag
    26941 gtgcgtctgc tgcgtgcgat tcaggagcgc gaagttcagc gtgtcggtag caaccagact
    27001 atctcggttg atgtccggct gattgcggcg acccatcgcg atcttgccgc agaggtgaat
    27061 gccgggcgtt ttcgccagga tctctactat cgcctgaatg tggtggcgat tgaagtacca
    27121 tcgctgcggc aacggcggga agatatccct ctgctggctg gccattttct gcagcgcttt
    27181 gccgagcgta atcgcaaggc ggtaaaaggt tttacgcccc aggcgatgga tctgttgatt
    27241 cattacgact ggccgggaaa tattcgtgag ctggaaaacg cggtggaacg ggcagtggtg
    27301 ctgctgccgg ggaatatatt tccgaacgcg agctgccgct ggcgattgcc agtacgccga
    27361 tcccgctggg gcaaagtcag gatattcagc cgctggtgga agtggaaaaa gaggtgattc
    27421 tggcggcact ggagaaaacg ggcggcaaca aaactgaagc cgcccgtcag ttagggatca
    27481 cgcgtaaaac gttattggca aaactgtcgc gttagttctg ctcgcgttcg atagcacgcc
    27541 agccgatatc tttccggcag aagcagtcgt cccagtggat atcggtcatt aaggcatagg
    27601 cgcgtttctg cgcttctgcc acggtatgac ccagcgcggt gacgcacagt acgcgcccgc
    27661 cgttggtcac tacctgctcg tcatccgcca gttttgtgcc cgcgtggaac actttgccgc
    27721 ctgccacttc ttccagcagc aggccgtgga tcacgtcacc ggtgcggtaa tcacccggat
    27781 atccacccgc agccatcacc acgccgagag aagcgcgttc atcccactcg gacgttttct
    27841 cgtccagttt gctttcacag gccgccaggc agagctcaac cagatcggac ttcatgcgca
    27901 gcataatcgg ctgggtttcc ggatcgccaa agcggcagtt aaattcaata accttcggat
    27961 tgccctgttt gtcgatcatc aggcccgcgt agagaaaacc ggtgtaggtg ttgccttccg
    28021 ccgccatgcc tttcacggtt ggccagataa tacgttccat ggtgcgctga tgaacgtcat
    28081 cggttactac cggcgcgggg gagtaagcgc ccatcccgcc ggtgtttggt ccggtatctt
    28141 tatcgcctac gcgtttgtga tcctggctgg tagccatcgg cagcacatgc tcgccgtcca
    28201 ccatcacgat aaagctcgct tcttcgccat cgaggaactc ttcgataacg atgcgatgac
    28261 ccgcgtcgcc aaaagcgttg cccgccagca tatcgtgaac agccgcttcc gcttcttcca
    28321 gcgtcatcgc cacgataacg cctttcccgg cagccagacc gtccgcttta atgacgattg
    28381 gcgcgccttt ctcacgcaga tacgccagcg caggttctac ctcggtgaag ttctggtatt
    28441 ccgccgtagg gatcttatgg cgggccagga aatctttggt aaacgctttt gagccttcca
    28501 gttgggccgc acctgcggtt gggccgaaga ttttcagccc ggcggcgcgg aaggtatcga
    28561 ccacgccttt caccagcggc gcttccgggc cgacgatggt cagatcaatc ttttcgtttt
    28621 gtgcgaaatc caacagcgcc gggatatcgg tcacgccaat agcaacgttt tgcagcgcgg
    28681 gttccagcgc agtgcctgca ttacccggag caacaaaaac agtctcaacc agcggcgact
    28741 gggccgcttt ccaggccagc gcgtgctcgc gcccgccgtt accaatcact aatactttca
    28801 tctattgctc cattaatggc ggaagtggcg catgtcggtg aagagcatcg caataccgtg
    28861 ctcgtcggcg gcggcaatca cttcgtcatc acggatggaa ccgccaggct ggattacgca
    28921 ggtcacgccc gcagcggcgg cggcatcaat accgtcgcgg aacgggaaga acgcgtcaga
    28981 agccatcgag gaacctttca cttccaggcc ttcatcggca gctttaatac cggcgatttt
    29041 cgcggagtac acgcggctca tctggcccgc gccaataccg atggtcatat tgtttttggc
    29101 atagacgata gcattggatt tcacgaactt cgccactttc cagcagaaca gcgcatcgcg
    29161 cagttcctgt tcggtcggct gacgttgggt gacgacgcgc agttcttccg caccgaccat
    29221 ccccaggtca cgatcctgaa ccagcagacc gccgttcacg cgtttgaaat caagacccgg
    29281 aacacgctcg ccccactgac cgcaggtcag aacgcgtacg ttctgcttgg cggcggtgat
    29341 tttcagggct tcttcgctgg cggaaggcgc aataatcact tcgacaaact gacgagaaat
    29401 gatggcctgt gcggtttccg catctagctc gcggttaaag gcaatgatgc cgccgaatgc
    29461 ggaggttggg tcggttttgt acgcgcgatc gtaagcatca agaatggaat tgccgatagc
    29521 tacaccgcaa gggttggcgt gcttcacaat cacacatgcc ggctcggcga actctttcac
    29581 gcactccagc gccgcatcgg tatcggcgat gttgttataa gagagggctt taccctgaac
    29641 ctgggttgcg gtagcaacgg aggcttcttt cacattctct tctatataga aggcagcctg
    29701 ctggtggctg ttctcgccgt aacgcatatc ctgcttctta atgaagttca ggttcagcgt
    29761 gcgtgggaag cgaccggcgg cttctttgct ttcaccgtgg tacgccggaa ccatgctgcc
    29821 gaagtagttg gcaatcatgc tgtcgtaggc ggcagtgtgt tcgaaggctt tgatggcgag
    29881 gtcgaaacgg gttgcaagcg tcagcgatcc ttcgttgtca tccatctctt taataatggc
    29941 gtcgtagtcg ctgctcttca ccacgattgc gacatcttta tggttcttag cggcggagcg
    30001 caccatggtc gggccgccga tatcgatgtt ctcaaccgca tcttccagcg agcagccttc
    30061 acgagcaaca gtctgggcga acggatacag gttaacaacc accatatcga taggctggat
    30121 ctgatgttct tccataatgg catcgtcctg gccgcgacgg cccagaatgc caccatgtac
    30181 tttcggatgc agggtcttca cgcgtccatc catcatctcc gggaaaccgg tgtaatcgga
    30241 aacttcggtt accggcagac ctttttctgc taacagacgg gcagtgcccc ctgtagacag
    30301 cagctccaca ccgcgtgcgg aaagtgcctg ggcgaattcg acgataccgg ctttgtcaga
    30361 aacactgagc agagcgcggc ggactggacg acgttgttgc atggtaaatc ccctggattt
    30421 gactattaca gagagcgtta gctgaatttt tcgcgaaaaa ctcagctaac gcccctaacg
    30481 gggcatcctt atttttcgcc cgcattgtaa cgaaaacgtt tgcgcaacgc tcgcgaattt
    30541 ttctctttca atggtgatca caattttgac agtggctacc gtgggaaaaa tacagaaatt
    30601 acattgatga ttgtggataa ctctgtgcgt aaaaaggtat aaagcggggt tttgctgggg
    30661 aatgcagcag tcagtcattt ttctgcaatt tttctattgc ggcctgcgga gaactcccta
    30721 taatgcacct ccatcgacac ggcggatgtg aatcacttca cacaaacagc cggttcggtt
    30781 gaagagaaaa atcctgaaat tcagggttga ctctgaaaga ggaaagcgta atatacgcca
    30841 cactcacgac agtgcgctaa
//
LOCUS       DADLFA010000029        29251 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000029 DADLFA010000000
VERSION     DADLFA010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29251
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.029"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..154
                     /locus_tag="JQH65_001115"
     CDS             <1..154
                     /locus_tag="JQH65_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2135 domain-containing protein"
                     /protein_id="HAY6138600.1"
                     /translation="NYYGGRSETELTTAQLTLITDEGSVNEKQETFIVPMRNAGELTL
                     VKSFDW"
     gene            429..3278
                     /gene="rcsC"
                     /locus_tag="JQH65_001116"
     CDS             429..3278
                     /gene="rcsC"
                     /locus_tag="JQH65_001116"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HAY6138601.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGEPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(3478..4128)
                     /gene="rcsB"
                     /locus_tag="JQH65_001117"
     CDS             complement(3478..4128)
                     /gene="rcsB"
                     /locus_tag="JQH65_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HAY6138602.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(4145..6817)
                     /gene="rcsD"
                     /locus_tag="JQH65_001118"
     CDS             complement(4145..6817)
                     /gene="rcsD"
                     /locus_tag="JQH65_001118"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HAY6138603.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVNTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTISDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQPQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            7556..8686
                     /gene="ompC"
                     /locus_tag="JQH65_001119"
     CDS             7556..8686
                     /gene="ompC"
                     /locus_tag="JQH65_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HAY6138604.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYVRLGFKGETQVTDQLTGYGQWEYQIQGNTSEDNKENSWTRVAFAGL
                     KFQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFG
                     LVDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGAAVSSS
                     KRTDDQNGSYISNGVVRNYIGTGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSL
                     GWANKAQNFEAVAQYQFDFGLRPSVAYLQSKGKNLGVINSRNYDDEDILKYVDVGATY
                     YFNKNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            8798..9853
                     /gene="apbE"
                     /locus_tag="JQH65_001120"
     CDS             8798..9853
                     /gene="apbE"
                     /locus_tag="JQH65_001120"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HAY6138605.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MAEIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDEMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GINGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            9927..10979
                     /gene="ada"
                     /locus_tag="JQH65_001121"
     CDS             9927..10979
                     /gene="ada"
                     /locus_tag="JQH65_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HAY6138606.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCALGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVLANAIGKPKAVRAVASACAANKLAIVIPCHRVVGGDGTLSGYRWGV
                     SRKAQLLRREAENEER"
     gene            10979..11629
                     /gene="alkB"
                     /locus_tag="JQH65_001122"
     CDS             10979..11629
                     /gene="alkB"
                     /locus_tag="JQH65_001122"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HAY6138607.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTIHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFYPLTTDCRYNLTFRQAGKKE"
     gene            11705..13348
                     /gene="yojI"
                     /locus_tag="JQH65_001123"
     CDS             11705..13348
                     /gene="yojI"
                     /locus_tag="JQH65_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HAY6138608.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIEMA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLMAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            13566..15212
                     /gene="mqo"
                     /locus_tag="JQH65_001124"
     CDS             13566..15212
                     /gene="mqo"
                     /locus_tag="JQH65_001124"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HAY6138609.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMLERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(15362..15850)
                     /gene="eco"
                     /locus_tag="JQH65_001125"
     CDS             complement(15362..15850)
                     /gene="eco"
                     /locus_tag="JQH65_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HAY6138610.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(15985..16149)
                     /locus_tag="JQH65_001126"
     CDS             complement(15985..16149)
                     /locus_tag="JQH65_001126"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6138611.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            16258..16752
                     /gene="napF"
                     /locus_tag="JQH65_001127"
     CDS             16258..16752
                     /gene="napF"
                     /locus_tag="JQH65_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HAY6138612.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            16742..17005
                     /gene="napD"
                     /locus_tag="JQH65_001128"
     CDS             16742..17005
                     /gene="napD"
                     /locus_tag="JQH65_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HAY6138613.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            17002..19488
                     /gene="napA"
                     /locus_tag="JQH65_001129"
     CDS             17002..19488
                     /gene="napA"
                     /locus_tag="JQH65_001129"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HAY6138614.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTLITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEDLLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTQDATDPLSKETDFKKCAVKLEKV"
     gene            19495..20190
                     /gene="napG"
                     /locus_tag="JQH65_001130"
     CDS             19495..20190
                     /gene="napG"
                     /locus_tag="JQH65_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HAY6138615.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHRDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            20177..21040
                     /gene="napH"
                     /locus_tag="JQH65_001131"
     CDS             20177..21040
                     /gene="napH"
                     /locus_tag="JQH65_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HAY6138616.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPRVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            21037..21486
                     /gene="napB"
                     /locus_tag="JQH65_001132"
     CDS             21037..21486
                     /gene="napB"
                     /locus_tag="JQH65_001132"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HAY6138617.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            21496..22098
                     /gene="napC"
                     /locus_tag="JQH65_001133"
     CDS             21496..22098
                     /gene="napC"
                     /locus_tag="JQH65_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HAY6138618.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            22117..22734
                     /gene="ccmA"
                     /locus_tag="JQH65_001134"
     CDS             22117..22734
                     /gene="ccmA"
                     /locus_tag="JQH65_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HAY6138619.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            22731..23393
                     /gene="ccmB"
                     /locus_tag="JQH65_001135"
     CDS             22731..23393
                     /gene="ccmB"
                     /locus_tag="JQH65_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HAY6138620.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            23435..24172
                     /gene="ccmC"
                     /locus_tag="JQH65_001136"
     CDS             23435..24172
                     /gene="ccmC"
                     /locus_tag="JQH65_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HAY6138621.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            24169..24378
                     /gene="ccmD"
                     /locus_tag="JQH65_001137"
     CDS             24169..24378
                     /gene="ccmD"
                     /locus_tag="JQH65_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HAY6138622.1"
                     /translation="MTLAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILCGVEQQRAREARLRAAQQQEAA"
     gene            24375..24854
                     /gene="ccmE"
                     /locus_tag="JQH65_001138"
     CDS             24375..24854
                     /gene="ccmE"
                     /locus_tag="JQH65_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HAY6138623.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            24851..26794
                     /locus_tag="JQH65_001139"
     CDS             24851..26794
                     /locus_tag="JQH65_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HAY6138624.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            26791..27348
                     /gene="dsbE"
                     /locus_tag="JQH65_001140"
     CDS             26791..27348
                     /gene="dsbE"
                     /locus_tag="JQH65_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HAY6138625.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            27345..28397
                     /locus_tag="JQH65_001141"
     CDS             27345..28397
                     /locus_tag="JQH65_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HAY6138626.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPDAFPEQSVPEGKRAGYIVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLSANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(28432..29079)
                     /gene="narP"
                     /locus_tag="JQH65_001142"
     CDS             complement(28432..29079)
                     /gene="narP"
                     /locus_tag="JQH65_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HAY6138627.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPSFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
BASE COUNT         6680 a         7220 c         8104 g         7247 t
ORIGIN      
        1 caactactat ggtgggcgca gcgaaacgga attaaccacc gcccaactga cgctgatcac
       61 cgatgaagga tcggtcaatg agaaacagga aacatttatt gtaccgatgc gtaatgctgg
      121 cgagctgacg ctggtgaaaa gttttgactg gtgatccatc ccttcacaca acctggctct
      181 ctcttttttc agataagact gctgggcgat ctgccgacgc caggatataa ccattaacaa
      241 gcgtcggcag attggtcatt tcttattttt cgtactgaga actgtccatc tactccggct
      301 ttcctggtta aggcaatggt ttctaagcga tacgcattat tgcaataatg acagaacatt
      361 gcggtaacac gcttttaccg ctaccttaac cacactccat cggtcacctg aggcggagct
      421 tcgccccttt gaaatacctt gcttcttttc gtacaaccct gaaagcctcg cgctacatgt
      481 tcagagcatt ggcgttagtg ctctggctgt tgattgcttt ttcatccgtt ttttacatcg
      541 ttaatgcgtt acatcagcga gaatcggaaa ttcgtcagga atttaatctg agttccgatc
      601 aggctcagcg ctttattcaa cgcacctctg atgtgatgaa agagctgaag tacatcgccg
      661 aaaatcgctt atcggcagaa aacggtgtgc tttccccgcg tggacgagaa acgcaggcgg
      721 atgtgcctgc gtttgaaccg ctgtttgccg actccgattg ttccgcaatg agtaacacct
      781 ggcgaggttc tctggagtca ttggcgtggt ttatgcgcta ctggcgcgat aatttttctg
      841 cggcttacga tctaaaccgg gtatttttaa tcggcagcga taacctctgc atggccaatt
      901 tcggtctgcg tgatatgcca gtggaacgcg ataccgcgtt gaaagctttg catgaacgca
      961 tcaataaata tcgaaatgca ccacaagatg atagcggcag taacctctac tggatcagcg
     1021 aaggtccgcg ccctggcgtc gggtattttt acgcgttgac gccagtttat ctggcgaacc
     1081 ggttgcaggc gcttttgggg gtcgagcaga ccatccggat ggagaacttt ttcttaccgg
     1141 gtacgttgcc gatgggggtt accattcttg atgaaaatgg tcataccctg atttcgctta
     1201 ccggaccaga aagtaaaatt aagggcgagc ctcgctggat gcaggaacgc tcctggtttg
     1261 gctatacgga agggttccgg gagctggtgc tgaagaaaaa tctgccaccc tcatcgctaa
     1321 gcatcgtgta ttcggtgccg gttgataagg tgctggaacg cattcgcatg ttgatcctta
     1381 acgcaatttt gctgaatgtg cttgccggag ctgcattgtt tactctcgca cggatgtacg
     1441 agcgacgtat tttcattccg gcggaaagcg acgccctgcg actggaagaa catgagcagt
     1501 tcaatcgcaa gattgtcgcc tccgcgccag tgggtatctg cattttgcgt accgctgatg
     1561 gcgtcaatat tttaagtaac gaactggcgc atacctatct caatatgctt acgcatgagg
     1621 accgccaacg actgacgcaa attatctgtg ggcagcaggt caattttgtt gatgtcctga
     1681 ccagcaacaa taccaatctg caaatcagct tcgtccattc gcgctatcgt aatgaaaacg
     1741 tggccatttg tgtgctggtg gatgtttctt cgcgcgtgaa gatggaagag tcgttgcagg
     1801 agatggcaca agcagcggaa caggcgagcc agtcaaaatc gatgttcctt gccaccgtca
     1861 gtcatgagct gcgaacgccg ctgtatggca ttatcggtaa cctggatctg ttgcaaacca
     1921 aagagttacc gaaaggcgtc gatcggctgg tgacggcaat gaacaactct tccagcctgt
     1981 tgttgaaaat tatcagcgat attctcgatt tctcgaagat tgaatcggaa cagttgaaga
     2041 tcgaaccgcg tgagttttca ccgcgtgaag tgatgaacca catcaccgcc aactatttac
     2101 cgctggtggt acgcaagcag ttaggcttgt actgctttat tgaaccggat gtgccagtgg
     2161 ccttaaatgg cgacccgatg cgtttacagc aggtcatctc caacctgttg agtaacgcca
     2221 taaaattcac cgataccggc tgtatagttt tgcatgtccg cgctgatggc gattatctct
     2281 ctatccgtgt tcgcgatacc ggcgtgggga ttccggcgaa agaagtggtg cgcttgtttg
     2341 atcccttctt ccaggtcgga acgggcgtac agcgaaactt ccaggggacc ggtctgggtc
     2401 tggcgatttg tgaaaaactg atcagcatga tggacggcga tatctcggta gattcagaac
     2461 cgggaatggg cagccagttt accgtgcgta ttccgttgta cggcgctcag tacccgcaga
     2521 aaaaaggcgt ggaagggttg agtggtaaac gctgctggct ggcggtccgc aatgcgtcgc
     2581 tctgtcagtt cctggaaact agtttgcagc gcagcggcat cgtcgttaca acatacgaag
     2641 ggcaggaacc gactcccgaa gatgtgttaa ttactgacga ggtagtgagt aaaaaatggc
     2701 agggtagagc ggtagtgacc ttctgtcgtc gccatattgg tattccgctg gagaaagcgc
     2761 caggggagtg ggtacacagt gtggctgccc cgcatgagct accggcattg ttggcgcgta
     2821 tttatttgat cgagatggag agcgacgatc ctgctaacgc tctgccgtcg acggacaaag
     2881 cggtcagcga taatgacgat atgatgattc tggtcgtgga tgatcatccg attaaccgac
     2941 gtctgctggc agatcagttg ggatcgttgg gctatcaatg taaaaccgcg aatgatggtg
     3001 tcgatgcgct taatgtactt agcaagaatc atattgatat cgtgcttagc gacgtcaaca
     3061 tgccaaatat ggatggttac cgcttgacgc aacgcattcg tcagttggga ctgacgttgc
     3121 cggtaatcgg agtaactgct aatgcgttgg ctgaagagaa gcagcggtgt ctggagtccg
     3181 gtatggacag ctgcctgtcg aagccggtaa cgctggatgt gataaaacag acgctgacgg
     3241 tatatgccga gagggtcagg aaatcgcggg aatcgtagga cggataaggt gtttatgtcg
     3301 catctggcat tcagtgcaaa tgccagatgc gacgctgacg cgtcttatct ggcctacttt
     3361 gatgtaatct gttgaaataa tgggaatcgt aggacgcata aggcgtttac gccgcatccg
     3421 gcaatcggtg caaatgccag ataagacact aacgcgtctt atctggccta caggtgatta
     3481 gtctttatct gccggactta acgtcactga agagagataa ttcagcaggg cgatatcgtt
     3541 ctcgacaccc agcttcatca tcgcagattt cttctggcta ctgatggttt taatactgcg
     3601 gttcagcttt ttagcgatct cggtcaccaa gaagccttcc gcaaacaggc gcagaacttc
     3661 actctctttt ggcgagagac gcttgtcacc gtaaccacca gcactgattt tttccaacag
     3721 gcgagaaacg ctttccgggg taaatttctt ccctttctgc agcgcggcga gagctttcgg
     3781 cagatcggtc ggtgcacctt gtttcagcac gatcccttcg atatccagat ccaataccgc
     3841 actaagaatc gccgggttgt tgttcatagt cagaacaatg atcgacaggc ttgggaaatg
     3901 gcgcttgatg tacttgatta aggtaatgcc atcgccgtac ttatcgccag gcatggagag
     3961 atcggtaatc aacacatgcg catccagttt cggcaggttg ttgatcagtg ctgtagagtc
     4021 ttcaaattcg ccgacaacat tcacccactc aatttgctca agtgatttgc gaataccgaa
     4081 caagactatc ggatggtcat cggcaataat tacgttcata ttgttcatgt aataggctac
     4141 cttgctacag caagctcttg acataactgt caatgtcgct gatgtatttt tctattcctg
     4201 gaacatcctt ctcacgaatc agatgttcca gcgtttcaca taactgcttg ccgggtacca
     4261 gatttagcat ggcaaatacg cctttaagac gatgagccgt ttgggctaac gcagcaaagt
     4321 cactggttgc tgcttcagta tacagcctct taacatcatc cggtactgtg tctacaaaga
     4381 gcgcataata gccgctggca tggagttgcg cattttcatc tccgcccaga ggcgattctg
     4441 tcacctcttc ctgcgccagt tgcacttcaa ttaattgtaa gaccgcttcc tgcatagcgt
     4501 tgctcatatt gaagttgacg cacaattgac caggcccaat ttcccgtacg ccagactcat
     4561 catcgcttaa aagcaagcca gaggcagtaa gattagacgg attatccgtt aaaaagatat
     4621 cataatcttg actaattaat ctttcatcgg gtgtgataca ggttgcaccc caattttcta
     4681 actggcgagt gacaatattc cgaatttctg ccgaagtaac atccaccatt acgcagacat
     4741 catccagtaa acgctcttct tcctcttcaa cttccgggtc agcggcgagc attttgatat
     4801 gcacagagta gcgtgtacca agcccatccc gcgttttgat gtttaaatga ccgcccagtt
     4861 tacgcgccag ttgatcgctc agccagaatg ccagcgggtc cgccttgcca tagcgatcgt
     4921 tttggggctg gttgataaac gggaagtgca aattatccat ttcatgaata cttacgcctt
     4981 ctcccgtgtc cagaatgcgg aatgtcaggc ggtcttcaga ggactcatcc tgatcaacct
     5041 caagggtgat ttttcccaat tgcgttgagg tcacggcata ttgcatcagt agcagcaaaa
     5101 tacgtcgtaa ggcatcgcga tcgccgcggc gcatatcgtg tgctttcaga tgattgttaa
     5161 tcagcagttg cagaccttta cgcttgatgg caggcaacac tgaaggcaca acttcatcaa
     5221 ttaaatcctg cacggagaac agcaccgtct cacttttcca gctatcgtcc gcaagcatgt
     5281 tcgctaactg tatttcatcg accagccgca ccagcacatc tgcctgattc gccagttgtt
     5341 tgctttccgg ggcgttgagt ttagccgcgc tctccgccag ggactgtgcg ggttctttca
     5401 gcgcatcgcc aatgtttttc ataaaggtca tccgcccctg ctggtttttc tcatacagac
     5461 gctgcgcctg cttgagtttc ttgtttacca gcacttcgcg atcctgatcg cgaataatga
     5521 aaatttgtgt gcgcggcgcg acctggctgc ggaacatgcg gatctcatac agctcgttat
     5581 tgatcgtcgc ctgaataatc ccctgatgct gttccgccat ggtggtgatg ttttgcagat
     5641 tcaaatgcgg cagcaaatga tcggcaattt tgttacttat gacagtgcgg ttcgattcct
     5701 gatcgtgaac cagcaggccg agcggcagca gtgagactat ctcttcatta attgcccgta
     5761 aaatgcgcaa ttcgttattg accgccgtgc tgggtagact ttctgtactg cggctggaga
     5821 aatggcggaa tgtggtatag ccaaataacg ccagcgccag caaaccgatg ttcagcagca
     5881 gtggcagcag aatgttttgc aacgtatcca gcaataaggt gccataagga acctgccaga
     5941 ccaggcgcat atcggtagag ttgagtgccg aggagatttc aatcttcgta ctgttaaagt
     6001 ggatactgac gctatccgtc ccttctttct cattatcatt gtctcccgtt gccgtcgcgt
     6061 ctggctcaag gcggaaactg tccagcggca tacccggtgg gatcaaatca ttaatcggta
     6121 gatcaaaagc tacgaccgtt gccagatgtc ccggctggtt gaacgtggta cgcaaggtaa
     6181 agtaatgacc gttctgccag gccaggcggc gcatgttaga aaaactttcg cgttcatcga
     6241 gggcgttggc ctgttgcaac atctctgcac gacgtgaatc aacaatgtcg ctgatggtcg
     6301 attctttaaa tccggaggtg agatctttga ggggcagggt tgagatcagc accagactgt
     6361 tatcctgacc attcaggtaa tacatcgacc acggtacatt ttctgcgccc cacaatgtgt
     6421 ccagataggt ggacatccgc tgagtcatct caagcgttga gttgtcgtga gagccaaaga
     6481 ttaacgcttc cgttttgcgg cgcggtttct ccagatagta gacatcctgt ttcaggcgcg
     6541 tctcttgtaa accttcgccg gaggaggggg aggttgtcgc ggcaatgttg tcgtagatct
     6601 gccaggtcac gtaacgccag gtattgacgc gcttttggat agcgtgggta atgtcgacaa
     6661 tctggtaact tttatctttc agccatgcgt taacggcgct ttgtaccatt acacccatcg
     6721 tcaccagtaa cacaatgatc aacagtaaaa agaagcgggt aatgctcccc ggtaggagtg
     6781 aaaagcgggt cgtggccgtt gtctctttct gacgcattcg tgtttatgac ctgttaaaac
     6841 ttcgcgaagt gatagggcag tataaagggt acagtgtgaa attccagact cttacctgat
     6901 tgatgattct ctattctccc gtgatattta tcatcctgca ttgggggaaa tagatgtaca
     6961 agcgccattt tgctgtacaa ataacctaca aaaagcccaa cgaaattaat tgcaaataaa
     7021 cgatgattgc tggaaattat gctgatgtta attatttgtg aaatagttaa caagcgttat
     7081 agtttttctg tggtagcaca gaataatgaa aagtgtgtaa agaagggtaa aaaaaaccgg
     7141 atgcgaggca tccggttgaa ataggggtaa acagacattc agaaatgaat gacggtaata
     7201 aataaagtta atgatgatag cgggagttat tctagttgcg agtgaaggtt ttgttttgac
     7261 attcagtgct gtcaaatact taagaataag ttattgattt taaccttgaa ttattattgc
     7321 ttgatgttag gtgcttattt cgccattcca caataatctt aaaaagttcc cttgtattta
     7381 cattttgaaa catctatagc gataaatgaa acatcttaaa agttttagta tcatattcgt
     7441 gttggattat tctgcatttt tggggagaat ggacttgccg actgattaat gagggttaat
     7501 cagtatgcag tggcataaaa aagcaaataa aggcatataa cagagggtta ataacatgaa
     7561 agttaaagta ctgtccctcc tggtcccagc tctgctggta gcaggcgcag caaacgctgc
     7621 tgaagtttac aacaaagacg gcaacaaatt agatctgtac ggtaaagtag acggcttgca
     7681 ctatttctct gacaacaagt cagaagacgg cgaccagacc tatgtacgtc ttggtttcaa
     7741 aggcgaaact caggttactg accagctgac cggttacggc cagtgggaat atcagatcca
     7801 gggcaatacc tctgaagaca acaaagaaaa ctcctggacc cgtgtggcat tcgcaggtct
     7861 gaaattccag gatgtaggtt ctttcgacta cggtcgtaac tacggcgttg tttacgacgt
     7921 aacttcctgg accgacgtac tgccagaatt cggtggcgac acctacggtt ctgacaactt
     7981 catgcagcag cgtggtaacg gcttcgcgac ctaccgtaac accgacttct tcggtctggt
     8041 tgacggtctg aactttgctg ttcagtacca gggcaaaaac ggtagcgtaa gcggcgaagg
     8101 catgaccaac aacggtcgtg gcgctctgcg tcagaacggc gacggtgtcg gcggttctat
     8161 cacttatgat tacgaaggct tcggtatcgg tgctgcagtt tccagctcca aacgtactga
     8221 tgatcaaaat ggtagctaca tcagcaatgg tgtagttcgt aactacatcg gtactggcga
     8281 ccgtgctgaa acctacactg gtggtctgaa atacgacgct aacaacatct acctggctgc
     8341 tcagtacacc cagacctaca acgcaactcg cgtaggttcc ctgggttggg cgaacaaagc
     8401 acagaacttc gaagctgttg ctcagtacca gttcgacttc ggtctgcgtc cgtctgtagc
     8461 atacctgcag tctaaaggta aaaacctggg tgtcatcaat agtcgtaact acgacgacga
     8521 agatatcctg aaatatgttg atgttggcgc gacctactac ttcaacaaaa acatgtccac
     8581 ctatgttgac tacaaaatca acctgctgga cgacaaccag ttcactcgtg acgctggcat
     8641 caacactgat aacatcgtag ctctgggtct ggtttaccag ttctaatctc gattgatatc
     8701 gaacaaaggg cctgcgggcc ctttgttcat tgttttcagc gtacaaactc agttttttgg
     8761 tgtactcttg cgaccgttcg catgaggata atcacgtatg gaaataagct ttacccgcgt
     8821 ggcactgctg gctgccgcgc tcttctttgt tggttgcgat caaaaaccac aacccgccaa
     8881 aacccacgct actgaagtta ccgttcttga aggcaaaacc atgggtacct tctggcgtgc
     8941 cagcatcccg ggcattgatg ccaaacgcag tgccgaactt aaagaaaaga ttcagaccca
     9001 gctggacgcc gacgatcagc tgctttcgac ctataaaaaa gattccgcgc tgatgcgctt
     9061 taacgactcg caaagtttgt cgccgtggcc agtaagtgaa gcgatggccg aaatcgtcac
     9121 tacctcgctg cgcattggcg cgaagaccga tggtgcgatg gatattaccg tcgggccgct
     9181 ggtaaatctg tggggctttg gcccggaaca acagccggtt caaattccga gccaggaaca
     9241 gatcgacgag atgaaagcca aaactggttt acagcacctt acggtcatta atcagtcgca
     9301 tcagcaatat ctgcaaaaag acctgccgga tttatatgtc gatctctcta ccgtcggcga
     9361 aggttatgcg gcggatcact tggcacgctt gatggagcag gaagggattt cccgctatct
     9421 ggtgtcggtg ggcggcgcgc tgaacagccg tggtatcaac ggtgaaggcc tgccgtggcg
     9481 ggtagcgatt caaaaaccaa ccgataaaga aaacgcagtt caggctgtgg tggatatcaa
     9541 cggtcatggg attagcacct ctggcagcta ccgtaactat tacgaactgg acggcaaacg
     9601 tctttcccat gttatcgatc cgcaaaccgg gcgtcccatc gaacacaatc tggtatccgt
     9661 gacggtgatt gctccgacgg cgctggaagc cgatgcctgg gatactggct tgatggtact
     9721 cgggccggag aaagccaaag aggttgttcg ccgggaaggg ctggcggttt atatgatcac
     9781 caaagaaggc gatagcttta aaacctggat gtcaccacag tttaaaagct tccttgtcag
     9841 cgaaaaaaat taaagcgcaa gattgttggt ttttgcgtga tggtgaccgg gcagactaaa
     9901 ggctatcctt aaccagggag ctgattatga aaaacgccac atgcttaact gacgatcaac
     9961 gctggcaatc tgtcttagcc cgcgacccga atgccgacgg cgaattcgtt ttcgccgtgc
    10021 gcaccacagg catcttttgc cgtccgtctt gccgcgccag acatgccttg cgggaaaatg
    10081 tctccttcta cgcaaatgcc agcgaggcac tcgccgccgg atttcgcccc tgcaaacgct
    10141 gccaacctga taaagccaat gcccagcaac atcggttgga taaaatcacc cacgcgtgtc
    10201 gactgctgga acaggaaacg cctgtaacgt tggaagcctt agccgaccag gtggcgatga
    10261 gtccatttca tctacatcgg ttgtttaaag cgactaccgg aatgacgcct aaagcctggc
    10321 aacaggcctg gcgcgctcgc cgtttgcgcg aatcgctggc gaaaggggag agcgtgacga
    10381 cgtctattct taacgccgga ttccccgaca gcagcagtta ctaccgcaaa gccgatgaaa
    10441 cgctgggcat gacggctaaa caattccgtc acggtggcga aaatctggcg gtgcgttacg
    10501 cgctggctga ttgtgcgctg ggtcgttgcc tggtggcaga aagcgagcgg gggatttgcg
    10561 cgatattgct gggcgatgat gacgcgacat taatcagcga gttgcagcag atgtttcccg
    10621 ctgccgacaa cgcgcctgcc gatctgacgt ttcagcaaca tgtgcgtgaa gtgatcgcca
    10681 gcctcaatca acgcgatacg ccgctgacgt taccgctgga tattcgcggc actgcttttc
    10741 agcaacaagt ctggcaggca ctgcgcacga taccttgcgg tgaaaccgta ctggctaacg
    10801 ccatcggcaa accgaaagcg gtacgggccg ttgccagcgc ctgtgccgcc aacaagctgg
    10861 ctatcgtaat accctgtcat cgagtggtcg gtggtgatgg cacactttcc ggttaccgct
    10921 ggggcgtgtc tcgtaaagcg caactgctgc gccgcgaagc tgaaaatgag gagaggtaat
    10981 gttggatctg tttgccgatg ctgaaccgtg gcaagagcca ctggcggctg gtgcggtaat
    11041 tttacggcgt tttgctttta acgctgcgga gcaactgatc cgcgatatta atgacgttgc
    11101 cagccagtcg ccgtttcgcc agatggtcac ccccggggga tataccatgt cggtggcgat
    11161 gaccaactgt gggcatctgg gctggacgat ccatcggcaa ggttatctct attcgcccat
    11221 tgatccgcaa acaaataaac cgtggcccgc catgccacaa agctttcatg atttatgtca
    11281 acgtgcggct acggcggcgg gctatccaga tttccagcca gatgcttgtc ttatcaaccg
    11341 ctacgctcct ggcgcgaaac tgtcgctgca tcaggataaa gacgaaccgg atctgcgcgc
    11401 gccaattgtt tctgtttctc tgggcttacc cgcgattttt caatttggtg gcctgaaacg
    11461 taatgatccg ctcaaacgtt tgttgttgga acatggcgat gtggtggtat ggggcggtga
    11521 atcgcggctg ttttatcacg gtattcaacc gttgaaagcg gggttttatc cactcaccac
    11581 cgactgccgc tacaacctga cattccgtca ggcaggtaaa aaagaataaa aataagaatt
    11641 attattgctg tgcgcgcgaa gatgtttaaa ctgcgggctg taattcattg tccgggtttt
    11701 ctgcatggaa cttcttgtac ttgtctggcg gcagtatcgc tggccattta tcagtgtgat
    11761 ggcgctaagc ctcgccagtg cggcattagg cattggctta attgctttta tcaatcagcg
    11821 ccttatcgaa atggcggata ccagtctgct ggtattgccg gagtttctgg gattattgct
    11881 gctgttgatg gcagtcactc tcggatcgca actggcgctc accactttgg ggcatcactt
    11941 cgtttaccga ctgcgtagtg aatttatcaa gcggattctg gatacccacg tcgagcgcat
    12001 tgaacaactc ggtagcgcct cgttgttggc gggattaacc agcgatgtgc gcaatatcac
    12061 cattgctttt gtgcgtcttc cggaactggt gcaggggatc attctcacta tcggttccgc
    12121 ggcgtatctg tggatgctgt cgggcaaaat gttgctggta acggctatct ggatggcgat
    12181 caccatctgg ggtggttttg tactggtggc gcgggtgtac aaacatatgg cgaccctacg
    12241 tgaaaccgaa gacaagctgt acacggattt tcaaactgta cttgaagggc gcaaagagct
    12301 gactctgaac cgggaacgcg ccgagtatgt gtttaacaac ctctacatcc ccgatgcgca
    12361 agagtatcgc catcatatta ttcgcgcaga caccttccat cttagtgccg tgaactggtc
    12421 aaacatcatg atgctgggcg ctatcggcct ggtgttctgg atggcgaaca gtctcggttg
    12481 ggctgatacc aacgttgctg cgacctattc gttgacgctt ttattcctgc gtacgccgct
    12541 gctttcggcg gttggcgcat tgccgacgct gctgatggcg caggtggcgt ttaacaagct
    12601 gaacaaattc gcgctcgcgc ctttcaaagc agagtttccg cgcccgcagg cgtttcccaa
    12661 ctggcaaacg ctggagctgc gtaatgtgac gtttagctat caggataacg cgttttccgt
    12721 tggtccgatt aatctcacca tcaaacgtgg cgagctactg tttctgattg gtggtaacgg
    12781 tagtggaaaa tcgacgctgg cgatgttgtt gacgggcttg tatcagccac aaagcggcga
    12841 aatcttgctg gatggcaaac ctgtcagcgg cgaacaaccg gaagattatc gcaaactgtt
    12901 ttcggcagtg tttaccgatg tctggctttt tgatcaactg ctggggccgg agggtaaacc
    12961 cgctaacccg caactggttg agaagtggct ggcgcagctg aaaatggctc ataagcttga
    13021 gttaagcaac gggcgtattg ttaacctgaa gttatcaaaa gggcagaaaa aacgcgtggc
    13081 gctgttgctg gcgctggcag aagaacgcga tattatcctg ctggatgaat gggcggcgga
    13141 tcaggatcca cacttccgtc gtgagtttta tcaggtgttg ctgccgctga tgcaggagat
    13201 gggtaaaact attttcgcta tcagtcatga tgatcattac tttatccacg ccgaccgcct
    13261 gctggaaatg cgcaatgggc aacttagcga gctgacgggc gaagagcgcg atgccgcttc
    13321 gcgtgatgcc gttgcccgga cggcataata ttacgacgct gacctggcgg actgctgccg
    13381 tcaggtcaat atgctaaaaa tcccttcatt tctaataacc ctataattaa tcaacgaaat
    13441 tataatgttt ctaaaattag aatataattt ataaacatta tttaaatgtt gttacttaag
    13501 tgttaccttt gatgccgcgc aaatctcacc tgcaataaag cgactaaaag taaggcatta
    13561 acaagatgaa aaaagtgact gccatgctct tctcgatggc cgtggggctt aatgccgttt
    13621 cgatggcggc aaaagcgaaa gcgtccgagg agcaggaaac tgatgtactg ttgattggcg
    13681 gcggcattat gagcgccacg ttggggacct atttacgcga gctggagcct gaatggtcga
    13741 tgaccatgct ggagcgcctg gagggtgtcg cgcaggagag ttcgaacggc tggaataacg
    13801 ccggaaccgg gcattctgca ctgatggaac tgaactacac cccgcaaaac gccgatggca
    13861 gtatcagtat tgaaaaagca gtcgccatta acgaagcatt tcagatttcc cgccagttct
    13921 gggcgcacca ggtcgagcgc ggcgtgctgc gtactccgcg ttcatttatc aataccgttc
    13981 cgcatatgag ctttgtctgg ggcgaggata acgttaattt cctgcgcgcc cgttacgccg
    14041 cgttgcaaca aagctcgctg tttcgcggta tgcgttactc tgaagatcac gcgcagatca
    14101 aagagtgggc accgttagtg atggaagggc gcgatccgca acagaaagtg gcagccacgc
    14161 gtacggaaat tggtaccgat gtgaactacg gcgagatcac ccgccagtta attgcttcct
    14221 tgcagaagaa atctaacttc tcgctgcaac tcagcagcga agtccgcgcc ctaaagcgta
    14281 atgacgataa cacctggacc gttaccgttg ccgatctgaa aaatggcact gcacagaaca
    14341 ttcgtgccaa atttgtcttt atcggcgcgg gcggtgcggc gctgaagctg ttacaggaat
    14401 cggggattcc ggaagcgaaa gactacgccg gtttcccggt gggcggacag ttccttgttt
    14461 cggaaaaccc ggacgtggtt aatcaccatc tggcgaaggt ttacggtaaa gcatccgttg
    14521 gcgcaccacc gatgtcggtt ccgcatatcg atacccgcgt tctggacggt aaacgcgtag
    14581 tgctgtttgg gccatttgcc accttctcaa ccaaattcct caaaaacggt tcattgtggg
    14641 atctgatgag ttccaccacc acctctaacg tgatgccgat gatgcacgtc gggctggata
    14701 atttcgatct ggtgaaatat ctggtgagtc aggtgatgtt gagtgaagag gatcgttttg
    14761 aagcgttgaa agagtactat ccgcaggcga aaaaagagga ctggcgtttg tggcaagcgg
    14821 ggcagcgtgt gcagattatc aagcgtgatg ccgataaagg tggtgtactg cgtctgggta
    14881 ctgaagtggt cagtgaccag caagggacca ttgccgcgct cctgggagca tcgccagggg
    14941 cgtcaaccgc cgcgccgatt atgttggatc tgctggaaaa agtatttggc gatcgcgttt
    15001 ccagcccgca atggcaggct acgttgaaag cgatcgttcc gtcttatgga cgcaagctga
    15061 acggtgatgt agcggcaaca gaacgcgagt tgcagtacac cagcgaagtg ctggggctga
    15121 aatacgacaa gccgcaggca gcagatagta cgccaaaacc gcagttgaaa ccgcaacccg
    15181 ttcaaaaaga agtggcggat attgcgttgt aatgatgtgc cacatccggc atggtatgcc
    15241 ggatgtggcg tatactgata agacgcgcca gcgtcgcatc aggcaaccgg ctcgggcgtt
    15301 agatgttgga tgcggcgtaa ccgccttatc caaccaacgg ggtgccgtac ttcacaacag
    15361 tttagcgaac ttctgcgttg tcaattttct cttccgcctt ccagacgcgg tatttcacat
    15421 ctacattgtc tggcgtatac accacgatcg gcagcttgct gttgtaacgc agcattccag
    15481 catcgcccag atacgcggtg acaaatttct tctctttctt gccatccggg caggccatca
    15541 tcgtggaaac cggcgagcta actttatcaa agacataata atcatagccc cagccttcca
    15601 gcgttttgct ttccagcttc ccgccgagac gatgcaaatt gcaatcgact tccagcgtct
    15661 gaccgattaa cagttctact ttcagggtag attcatcttc ttgcggggtt aactgaatca
    15721 cctgacgctt catccctttt tcagcttgtg gataaggcgc gattttttcc agtggctgga
    15781 cgctttctgc cgcccaggcg gaagtggtag cgaaagcggc aaacaatact gcaggtagaa
    15841 tggtcttcat ttttttgctt tccttcttca tttataggtt ttcaccgatg atgtcgctga
    15901 aggttaacat attcataaga tttattaatt tgctttatga ataacttaaa gaatattcag
    15961 acaattttac ctgctctttt agtgttaaat tctgatgaga gaacaaacca gcagaaatat
    16021 ctctggtgaa ttaatcgcta tataaatatg tttataacca tttgaaatgt gagcaaaagc
    16081 ccgtttttgc cacgcttccc gccactcttt tgatcctgct acaggtttta ccccgatcgg
    16141 ggtatgcatc tttgacacat cctttaatat cttagcggtt ataaaaatgg cttattaatt
    16201 atgcggttta tttggtcgct ctcaattttc agagcgcgtt aatgatggaa ggtcaatgtg
    16261 aagattgatg catcccgtcg gggcatactc actggtcgct ggcgcaaagc cagtaacggt
    16321 atccgtccgc cctggtccgg tgatgaatct cattttctga cccattgcac ccgttgcgac
    16381 gcctgtatta atgcttgtga aaacaacatt ctgcaacgcg gcgcgggcgg ctatccgagc
    16441 gttaatttca aaaataacga gtgcagcttc tgttacgctt gcgcgcaggc ttgccccgaa
    16501 tcgctatttt ctccgcgcca caccagggcc tgggatttgc agttcaccat tggggacgcg
    16561 tgcctggcgt atcagtcagt tgaatgccgc cgctgtcagg atagctgtga gccaatggca
    16621 attatctttc gcccgacgct ctccgggatc taccagccgc aacttaatag ccaactttgc
    16681 aacggatgtg gagcatgtgc agccagctgt ccggtatcag ccataactgc ggagtatctc
    16741 catgcacact aactggcaag tttgcagcct ggtcgtgcag gccaaaagcg aacgaatttc
    16801 agacatcagc acccaactga acgcctttcc cggctgtgaa gttgctgtca gcgacgcgcc
    16861 gagcggtcag ttgattgtgg tggtggaagc agaagacagc gaaacgctga tccaaaccat
    16921 tgagtcagta cgcaacgtag agggcgtgct ggcggtgtcg ctggtttatc accagcagga
    16981 agagcaaggt gaggaaacac catgaaactc agtcgtcgta gctttatgaa agctaacgcc
    17041 gttgcggccg ctgcggcggc tgccggtctc agcgtgccgg gcgttgcccg cgccgttgtt
    17101 ggtcagcagg aagccatcaa atgggataaa gcgccgtgcc gtttctgcgg tactggttgc
    17161 ggcgttctgg tcggaacgca gcagggacgt gtggtggcct gtcagggcga cccagacgca
    17221 ccggttaacc gtggcctgaa ctgcattaag ggctatttcc tgcccaagat catgtacggt
    17281 aaagaccgtt tgacgcagcc gctgctgcgt atgaaaaacg gtaaatatga caaagaaggc
    17341 gaatttaccc taatcacctg ggatcaggcc ttcgatgtga tggaagagaa attcaaaacc
    17401 gccctgaaag aaaaagggcc ggaatcgatc ggtatgttcg gttctggtca gtggactatc
    17461 tgggaaggtt atgccgcgtc caagctgttt aaagcgggct tccgttcgaa caacatcgac
    17521 ccgaacgcgc gtcactgtat ggcgtcggca gtagttggct ttatgcgtac ctttggtatg
    17581 gatgagccga tgggctgcta tgacgacatc gagcaggctg acgcgtttgt gctgtggggc
    17641 gcaaacatgg cggagatgca cccgatcctc tggtcgcgca tcactaaccg tcgtctctct
    17701 aatcagaacg tcaccgtggc ggtgctttct acctaccagc atcgtagctt cgagctggcg
    17761 gataacggca tcatctttac gccacagtct gacctggtga tcctgaacta catcgccaac
    17821 tatatcattc aaaacaatgc gataaatcag gacttcttca gcaaacacgt taacctgcgc
    17881 aaaggggcga cggacattgg ctacggttta cgtccgaccc atccgctgga aaaagcagcg
    17941 aagaatccag gttctgacgc ctccgaaccg atgagctttg aagattacaa agccttcgtt
    18001 gccgagtata cgctggaaaa aactgccgaa atgaccggcg tgccgaaaga ccagttagaa
    18061 caactggcgc agctgtatgc cgatccgaac aagaaagtca tctcctactg gacgatgggc
    18121 ttcaaccagc atactcgtgg cgtgtgggct aacaacctgg tctacaacct gcacctgctg
    18181 accggcaaaa tttcccagcc gggttgcggt ccgttctccc tgaccgggca accttctgcg
    18241 tgtggtactg cgcgtgaagt gggcaccttt gctcaccgtc tgcctgcgga catggtggtg
    18301 accaacgaga aacaccgtga tatctgcgag aagaagtgga atatcccgag cggcaccatt
    18361 ccggcgaaaa tcggtctgca cgcggtggca caagaccgtg cgctgaaaga cggcaaactg
    18421 aatgtttact ggaccatgtg taccaacaac atgcaggccg ggccgaacat caatgaagag
    18481 cgtatgccgg gctggcgcga tccgcgcaac ttcatcatcg tctccgatcc gtatccgaca
    18541 gtcagtgcgc tggccgccga cttgatcctg ccgaccgcaa tgtgggtaga gaaagagggc
    18601 gcttacggta acgccgaacg ccgtactcag ttctggcgtc aacaggtaca ggcaccgggc
    18661 gaagcgaaat cggatctctg gcagttagtc cagttctccc gccgcttcaa aactgaagaa
    18721 gtatggccgg aagatctgct ggcgaagaaa ccggaactgc gtggcaaaac gctgtacgaa
    18781 gtgctgtatg ccacaccgga agtgagcaaa ttcccggtat ccgaactggc ggaagatcag
    18841 ctgaacgatg aatcccgcga gctgggcttc tatctgcaaa aagggctgtt cgaagagtac
    18901 gcatggtttg gtcgcggtca cggtcacgat ctggcaccgt tcgatgacta ccacaaagcg
    18961 cgcggtctgc gctggccggt ggtgaacggt aaagaaacgc agtggcgtta cagcgaaggt
    19021 aacgacccgt acgtgaaagc gggcgaaggc tataagttct acggcaaacc ggatggcaaa
    19081 gcggtgatct tcgcactgcc attcgaaccg gcggcggaag caccggatga agagtacgac
    19141 ctgtggctct ctaccggacg cgttctggag cactggcaca ccggcagtat gactcgccgt
    19201 gtaccagaac tgcaccgcgc cttcccggaa gcggtcctgt ttattcaccc gctggatgcg
    19261 aaagcgcgcg atctgcgtcg tggcgacaaa gtgaaagtgg tttctcgccg tggcgaagtg
    19321 atctcgattg tcgaaacgcg cggtcgtaac cgtccgccgc agggcctggt gtacatgccg
    19381 ttcttcgacg ccgcacagct ggttaacaaa ctaacgcagg acgcgaccga tccgctctcg
    19441 aaagagacgg acttcaagaa gtgcgcagtc aaactggaga aggtgtaagc cattatgtcc
    19501 cggtcagcga aacctcaaaa tggtcgccgc cgctttctgc gcgatgttgt tcgcacagca
    19561 ggcgggctgg ctgccgtggg tgtggcgctg gggttacaac agcaaaccgc acgcgcatct
    19621 ggcgtgcggt tgcgcccgcc cggagccata aacgagaacg cctttgccag tgcctgtgtg
    19681 cgttgcggtc agtgtgttca ggcttgccct tacgacacct taaaactggc gacgctggcc
    19741 tctggtctgt cggcgggcac gccatatttt gtcgcacggg atattccttg cgaaatgtgt
    19801 gaggacattc cgtgcgccaa agtgtgcccg agcggtgcgc tggatcgtga gatcgaatcg
    19861 atcgacgacg cgcggatggg gctggcggta ctggtggacc aggaaaactg tctcaacttt
    19921 caggggctgc gctgcgatgt ttgttatcgc gaatgcccga aaattgatga ggccatcacc
    19981 ctggagctgg agcgcaacac gcgtaccggt aagcacgccc gctttctgcc gacggttcac
    20041 agagacgcct gtaccggttg cggtaagtgc gaaaaagtgt gcgtgctgga acaaccggca
    20101 atcaaggtgt taccgctgtc actggctaaa ggggagttag gtcaccatta ccgcttcggc
    20161 tggctggagg ggaacaatgg caaatcgtaa acgtgacgcc gggcgcgagg cgctggagaa
    20221 aaaaggctgg tggcgcagtc accgttggct ggtgttacgt cgcctttgtc agttcttcgt
    20281 gctggggatg tttttgagcg gtccgtggtt tggtgtgtgg atcttacacg gtaactacag
    20341 cagtagcctg ttattcgaca ccgttccgct gaccgatccg ctgatgacgc tgcaaagtct
    20401 tgccagcggt catctgcctg ccacggtggc gttgaccggt gcggtgatta tcaccgtgct
    20461 ctacgccctg gcaggaaagc gattattttg cagctgggtc tgcccgctga atccgattac
    20521 cgacttagcg aactggttac gcaggcgctt tgacctcaat cagtctgcga cgatccctcg
    20581 ccacattcgc tacgtgctgt tggtggtcat tctgctggga tcggccttaa ctggcacgct
    20641 catctgggaa tggatcaacc ccgtatctct gatggggcgc agcctggtaa tgggcttcgg
    20701 cagcggcgcg ctgcttattc tcgcgctgtt tttatttgat ctactggtcg ttgaacacgg
    20761 ctggtgcggg cacatttgtc cggttggcgc gctgtatggc gtgctgggta gcaaaggtgt
    20821 gattaccgtt gcggccaccg atcggcagaa atgtaaccgc tgtatggatt gttttcatgt
    20881 ttgcccggaa ccgcgtgtgc tacgtgcccc ggtgctggat gaacaaagcc cggtgcaggt
    20941 caccagccgc gattgcatga cgtgcggtcg ctgcgtggat gtctgttctg aggatgtatt
    21001 tacaataact acacgatgga gttcgggagc gaaatcatga aaagccatga cctgaagaaa
    21061 gcgctgtgtc aatggacggc gatgctggcc ctggtggtaa gcggcgcggt ttgggcggct
    21121 aatggcgtcg attttagcca gtcgccggaa gtatccggga cacaggaagg ggccattcgg
    21181 atgccaaaag agcaggaacg gatgccgctg aactatgtga atcagccgcc aatgatcccg
    21241 catagcgttg aaggttatca ggtaacgacc aataccaacc gctgcttgca gtgccacggt
    21301 gtcgaaagtt atcgcaccac tggcgcgccg cgcattagtc ctactcactt tatggacagc
    21361 gacggcaaag tgggcgcgga agtggctccg cgtcgttatt tctgtctgca atgtcacgta
    21421 ccgcaggccg ataccgcgcc aatcgtgggg aataccttta ccccatcaaa aggttacggg
    21481 aaataagagg ttattatggg aaattctgac cgtaagcctg gtctgattaa gcgcctgtgg
    21541 aaatggtggc gtacccccag ccgtctggcg ctggggacgc tgctgttgat cggttttgtt
    21601 ggcggcatcg tcttctgggg tggctttaac accgggatgg aaaaagccaa taccgaagag
    21661 ttctgcatta gctgccacga aatgcgcaac acggtatatc aggaatacat ggattccgtg
    21721 cactacaaca accgtagcgg cgtccgtgcg acctgtccgg attgtcacgt tccgcacgag
    21781 tttgtgccga agatgatacg caagctcaaa gcaagtaaag agctgtatgg taaaattttt
    21841 ggcgttattg acacgccgca gaaatttgaa gctcatcgtc tgacgatggc acagaatgag
    21901 tggcggcgca tgaaggacaa taactcgcag gagtgccgta actgtcacaa cttcgagtat
    21961 atggatacaa ccgcccagaa atcggttgcc gcgaagatgc atgaccaggc ggtgaaagat
    22021 gggcaaacct gtattgattg ccataaaggg atagcgcaca agctgcccga tatgcgtgaa
    22081 gtcgagccag gtttttaaca gggttattgc gtgggtatgc ttgaagccag agagttactt
    22141 tgtgagcggg atgaacgaac cttatttagt ggcttgtcat ttacgctgaa cgcaggagag
    22201 tgggtacaaa tcaccggtag caacggcgcg gggaagacaa cgcttctccg tttgctgacg
    22261 gggttgtctc gccctgacgc aggcgaggtt ctctggcaag ggcagccctt gcatcaggta
    22321 cgcgacagct accatcaaaa cctgttatgg ataggccatc agccggggat caaaacccgg
    22381 ctgacggcgt tagaaaatct gcacttttat catcgcgatg gcgataccgc acaatgtctg
    22441 gaagccctgg cgcaggccgg gcttgccgga ttcgaagata ttcctgtaaa tcagctctcg
    22501 gccgggcaac aacgccgcgt cgctttagcg cgtctgtggc tgacccgtgc cacgttatgg
    22561 atcctcgacg agccttttac cgcgattgac gttaacggcg tcgatcgtct gacccagcgt
    22621 atggcgcagc atacggagca gggggggatt gtgattctga ctacccacca gccgctcaac
    22681 gttgctgaaa gtaaaattcg ccgcatttca ctgacgcaaa cgagggccgc atgatgttct
    22741 ggcgcatttt ccgtcttgag ctgcgtgtag cgtttcgcca tagcgccgaa atcgccaacc
    22801 cgctgtggtt cttcctgatt gtaattaccc tttttccgct cagtatcggt ccggagccgc
    22861 aactgctggc gcgtattgca ccgggcatta tctgggttgc tgcgctgctt tcatccttgc
    22921 tggcgctgga acgactgttc cgtgacgatt tgcaggacgg cagtcttgaa caattgatgt
    22981 tgttgccgtt acccttgccc gccgttgtgc tggcgaaggt gatggcgcac tggatggtaa
    23041 ccggtctgcc gttactcatc ctttcgccac tggtagcaat gctactggga atggatgttt
    23101 atggctggca agtgatggcg ctgacgctgc tgctgggaac gcctacgctt ggctttctcg
    23161 gtgcaccggg tgtggcgttg acagtgggac ttaagcgcgg tggtgtgctg ctcagcatac
    23221 tggtgttacc gctgactatc ccattactca tctttgccac cgccgcgatg gacgcggctt
    23281 ccatgcattt gcccgttgac gggtatctgg caattttagg cgcgttgctg gcaggcaccg
    23341 cgacattaag tccttttgcg acggcggcag cgttacgaat cagcattcaa taacgctgga
    23401 ttactgattt ttgttcgagt ctggtatcga aactatgtgg aaaacactgc atcaactggc
    23461 gatcccacca cggctgtatc aaatctgtgg ctggtttata ccgtggctgg caattgccag
    23521 tgtggtcgtg cttaccgtcg gctggatctg gggattcggc tttgctccgg ctgattatca
    23581 gcagggaaat agctaccgca ttatctacct gcatgtgcct gcggcgatct ggtcgatggg
    23641 tatttatgca tcaatggcgg tggcggcgtt tattggcctt gtctggcaga tgaaaatggc
    23701 caacctggcg gtggcggcga tggcccccat tggtgccgtg tttaccttta ttgccctggt
    23761 taccggctct gcatggggaa aaccgatgtg gggcacctgg tgggtatggg atgcacgtct
    23821 gacttctgaa ctggtgctgc tgtttttgta tgtgggtgtg attgccctgt ggcacgcctt
    23881 cgacgaccgc cgtctggcgg gccgtgcggc aggtatcctg gtgctgattg gcgtggtgaa
    23941 tctgccgatt attcattact ccgtggagtg gtggaacacc ctgcatcagg gatcaacgcg
    24001 gatgcagcaa agtatcgatc cggcgatgcg ttcgccgctg cgctggtcga tttttggctt
    24061 cctgctcctg tctgccacgc tgacgctgat gcggatgcgt aatttgattt tgctgatgga
    24121 aaaacgccgt ccgtgggtga gtgaactgat actgaaaaga ggccgtaaat gacccttgca
    24181 tttgcttcct ggaatgaatt tttcgcaatg ggcggttacg ccttttttgt ctggctggcg
    24241 gtggtgatga ccgttattcc gctggtggtt ttggtcgtgc actcggtgat gcagcatcgc
    24301 gcaattctgt gtggtgtgga gcaacagcgg gcgcgtgagg cgcgtttacg tgctgcgcaa
    24361 cagcaggagg ctgcatgaat attcgccgta aaaaccgctt gtggattgcc tgtgctgtgt
    24421 tggcaggttt ggcgctgacc atcggtctgg tgctgtatgc gctgcgctcg aatatcgatc
    24481 tcttttatac gccgggggaa attctctacg gcaagcgtga aactcagcaa atgccggaag
    24541 tcggtcagcg tctgcgcgtt ggcgggatgg tgatgccggg tagtgtgcag cgcgatccca
    24601 attcgctgaa agtgaccttc accatttacg acgctgaagg ctcagtggat gtctcttacg
    24661 aaggcatttt gccggatctg ttccgtgaag ggcagggcgt tgtggtgcag ggcgagctgg
    24721 aaaaaggcaa tcatatcctc gcgaaagaag tgctggcgaa acacgacgaa aactacacgc
    24781 cgccagaagt tgagaaagcg atggaagcta accaccgtcg cccggcgagt gtttataagg
    24841 acccagcatc atgatgccgg aaattggaaa cgggctgctg tgcctggcgc tgggaattgc
    24901 gctgctgctg tccgtgtatc cgctatgggg cgtggcgcgc ggagatgcgc gcatgatggc
    24961 gtcttcccgc ttgtttgcct ggctgctgtt tatgtctgtg gctggcgcat ttctggtgct
    25021 ggtcaatgct ttcgtggtca acgactttac cgtcacctat gttgccagca actccaatac
    25081 ccagcttccg gtgtggtatc gcgtggcggc tacctggggc gcgcatgaag gttcgcttct
    25141 gctgtgggtg ctgctgatga gcggctggac ctttgcggtg gcgattttta gtcagcgtat
    25201 tccgctggat attgtggccc gtgtactggc gataatgggg atggtcagcg tcggcttttt
    25261 gctgttcatt ctctttacct ctaacccgtt ctcacgcacg ctgccgaact tcccaattga
    25321 agggcgcgat cttaacccgc tgttacagga tccggggctg atcttccatc cgcctctgct
    25381 ttatatgggg tacgtgggtt tctcggtggc gtttgctttt gccattgctt ctttgctgag
    25441 cgggcgtctg gacagcactt atgcgcgttt tactcgcccg tggacgctgg cggcgtggat
    25501 tttcctgacg ctcggcattg tgctcggttc cgcatgggcc tattacgaac tcggctgggg
    25561 gggctggtgg ttctgggatc cggtagaaaa cgcctcgttt atgccgtggc tggtggggac
    25621 tgcgctgatg cactcactgg cggtcactga acaacgcgcc agcttcaaag cgtggacatt
    25681 actgctggca atcagtgcct tctcgttgtg tctgctgggg actttcctgg tgcgttccgg
    25741 cgtgctggta tcggtacacg cgtttgcctc tgatccggca cgcggtatgt ttatcctcgc
    25801 ctttatggta ctggtgattg gtggttcgct gctgctgttt gccgcgcgtg gacacaaagt
    25861 tcgctcacgc gtaaacaatg cgctgtggtc gcgggaatct ctgctgttag cgaacaacgt
    25921 tttgttggtc gccgcgatgc tggtggtatt actggggacg ctgctgccgc tggtgcacaa
    25981 gcaactggga ctgggcagta tttcgattgg cgaaccgttc ttcaacacca tgtttacctg
    26041 gctgatggtg ccgtttgcgc tgctgcttgg tgtcggtcct ctggtgcgct gggggcgcga
    26101 tcgcccacgt aaaatccgca atttattgat tatcgccttc atctcaacgc tggtgctgtc
    26161 gctgctgctg ccgtggctgt tcgaaagcaa agttgtggcg atgacggtgc tcggcctggc
    26221 aatggcctgc tggattgctg tgctggcaat tgcggaagct gcgctgcgta tttcacgtgg
    26281 cacgaaaacc accttcagtt attggggaat ggtggcggct cacctggggc tggcagtgac
    26341 aattgttggt attgccttta gccagaacta tagcgttgag cgtgatgtgc gcatgaagtc
    26401 cggcgatagc gtcgatattc atgaatatcg cttcaccttc cgtgatgtca aagaggtgac
    26461 tggcccgaac tggcgcggcg gtgtggcgac tattggcgta acgcgagagg gtaaaccgga
    26521 aacggtgctg tatgcggaaa aacgttatta caacactgcc gggtcgatga tgaccgaagc
    26581 ggcgattgac ggcggcatca cgcgtgacct gtacgccgcg ctcggtgaag agctggaaaa
    26641 cggcgcgtgg gccgtgcgtc tttactacaa accgtttgtt cgctggattt gggcgggcgg
    26701 gctgatgatg gcgttgggcg gactgctgtg tctgtttgat cctcgctatc gtaagcgcgt
    26761 gagtccgcaa aaaactgcgc cggaggctgt atgaagcgca aagtattgtt aattccgttg
    26821 attatcttcc tggcgattgc cgcggcgctg ctgtggcagc tggcgcgtaa tgccgaaggg
    26881 gatgatccga ccaatctgga atcggcgctc attggcaagc cagtgccgaa gtttcgcctc
    26941 gaatcactgg acaatccggg gcagttttat caggcggatg tgctgactca gggcaaacct
    27001 gtgctgctta acgtttgggc tacctggtgt ccgacctgcc gtgcggaaca tcaatatctg
    27061 aatcagcttt ctgcgcaggg cattcgcgtg gtcggcatga actataaaga cgatcgccag
    27121 aaggcaataa gctggctgaa agagctgggc aatccttacg cgctaagcct gtttgatggc
    27181 gatggcatgt tagggctgga tctcggtgtc tatggcgcgc cagaaacgtt cctcatcgac
    27241 ggcaacggca tcattcgcta tcgccatgcg ggcgatctca atcctcgcgt ctgggaagaa
    27301 gagatcaagc cgctgtggga gaaatacagt aaggaggccg cacaatgagg tttttattgg
    27361 gcgtgctgat gctgatgatc tccggctcag cgctggcgac catcgacgtg ttgcagttta
    27421 aggatgaagc gcaggagcag cagttccgcc aactcactga agaactacgc tgcccgaaat
    27481 gccagaacaa cagcattgcc gattccaact cgatgattgc caccgacctg cgccagaaag
    27541 tgtatgaact gatgcaggaa ggtaaaagta aaaaagagat tgtcgattat atggtggcgc
    27601 gttacggcaa cttcgtcact tacgatccgc cgttaacgcc gctgaccgtg ctgctgtggg
    27661 tgcttccggt agtggctatt ggcattggcg gttgggtcat ttacgcccgt tcgcggcgtc
    27721 gggtacgcgt ggtgccggac gcgtttcctg aacaaagcgt gccggaaggt aagcgtgccg
    27781 gatatattgt ttatctgccg ggtattgtgg tggcgttaat tgtggctggc gtcagctact
    27841 accagactgg caattatcag caggtgaaaa tctggcagca ggccacggca caggctccgg
    27901 cgttgctgga cagggcgctg gatccgaaag ccgatccgct caacgaagaa gagatgtcgc
    27961 gtcttgcgct ggggatgcgt actcaactgc aaaaaaatcc gggagatata gaaggctgga
    28021 ttatgttggg ccgcgttggc atggcgctgg gtaacgccag tatcgccacc gatgcatacg
    28081 ctactgcgta tcgcctcgat ccgaaaaaca gtgatgccgc actgggttat gctgaagcgt
    28141 tgacacgttc atctgatccc aacgacaacc gcctcggtgg tgaactgctg cgccagttgg
    28201 tgagaacgga ccatagcaat atccgtgtgt taagcatgta tgcgtttaat gcctttgagc
    28261 agcagcgatt tggcgaagcc gttgccgcgt gggagatgat gttgaaactc ttatctgcca
    28321 acgatactcg ccgtgcggtg attgaacgta gtatcgcgca ggcgatgcaa catttgtcgc
    28381 cgcaggagag taaataaaaa gaggcccgat ggtgatgcca tcgggctatt tttattgtgc
    28441 cccgcgttgt tgcaggaaca gaatggtggc tgccacgcgt gagcggacat tgagtttgcg
    28501 cagcagattg cgaatatgta cttttactgt ctgctctgaa atattcaaca ccgaggcaat
    28561 ctgtttattt gacagcccct gtgccagctc gtgcagaaca tccagctcgc gctccgtcag
    28621 cacgctgaag ggatcttctt ccgcgccaaa catttcacgt tcacgtaagt actgattgac
    28681 gcgttcgcta aagactttgc tgcctttcgc tccagcacga attgcttcca gcaatacttc
    28741 cgggtcgctg tctttcaaca gataaccgtc tgcgcctgcg tctatcagcg caaagacatc
    28801 gctggaggca tctgatacgg tcaggataat aatttgcgcg gtaacgccat ccctgcgcaa
    28861 ggcattgagg gtatccaggc cactcatacc tttcatattg agatccagca agatcacgtc
    28921 gatatccagt ctatttgcca gatcgatcgc gctcgcgccg tcgcccgctt cggcgaccac
    28981 ttcaaagcta ggatcaagct ccagtaactg acgaacaccg cgtcgcataa gtggatgatc
    29041 atccacaatc atcacctgaa aaggcgttgc ttcaggcata gtagtctcct ggggttttat
    29101 tagaatcatt atgtttgagc gtagcgttgg taacgctacc aggcgcgtga atgttacccc
    29161 attttatgta tggacatgta gtgaaaacta caaccttgtc gttcgctttg gcatcatttt
    29221 cattataaag acggaatttt gcatcagctt c
//
LOCUS       DADLFA010000030        28471 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000030 DADLFA010000000
VERSION     DADLFA010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28471
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.030"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..193)
                     /locus_tag="JQH65_001143"
     CDS             complement(<1..193)
                     /locus_tag="JQH65_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HAY6138628.1"
                     /translation="MFRCPLCGASARIRTSRPENDSNTVRQKYYQCNNLECGVCFSTL
                     EAFHKFTSKHASPIHSSEGI"
     gene            complement(329..1651)
                     /locus_tag="JQH65_001144"
     CDS             complement(329..1651)
                     /locus_tag="JQH65_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005113344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6138629.1"
                     /translation="MRICTCLYLTSRINTSDKLMTHYIKLILEGKLIDQTGGYETPTE
                     EIDNASQLIKILIRICLWTWSENMEHNGWMNSLARRLRDYDKTDMVMGRVYSNVFDCG
                     FIDMEQSWVQLLLIFSKKNDSVSKEIKEAIENNYITYREKQRLIGVLSKICNSIEYTK
                     IKLTLTLDDLQTKKENLRKLLQEHINMLKKDLDMRLQDAAIDVHRLDSTARKTSEHLR
                     KRIKKTLPLSLFKSIDFKQASDCFTKHKISIKIDKEPYAEGIESIPYINEGDIQADLI
                     LKDIQRIILSNLFSTGCSQHTVIEDFNMLIDHIKSSADLAGKLVLVMSKEIFQQYNRM
                     LFDNPNLRELMRKNDDGSMNITTESGTRKVYFLPFVNQPFSLVVKDNYFTKLIIREYD
                     NNKLVNVTSENIKSDSDKFKLTLNYELNIVFEGNADLKIAHSQRVTSE"
     gene            complement(1727..3447)
                     /locus_tag="JQH65_001145"
                     /pseudo
     CDS             complement(1727..3447)
                     /locus_tag="JQH65_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005113346.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(3696..4805)
                     /locus_tag="JQH65_001146"
                     /pseudo
     CDS             complement(3696..4805)
                     /locus_tag="JQH65_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
     gene            4963..6147
                     /locus_tag="JQH65_001147"
     CDS             4963..6147
                     /locus_tag="JQH65_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HAY6138630.1"
                     /translation="MSETRFHGARVTENTDLVTAINDVDSSVIGIVATADDADAKLFP
                     LNKPTLLTRVNDVLGKCGTTGTLYRALKAIADQVSTKVIVVRVAEHKEEDGKTQDQLV
                     IGGSEDDGSYTGMYALLVAEQDESIGYRPRILAAPELDTEAVTKSLCVIADKLRAFVY
                     ASCHGCNTMAEAITYRQKFNEREVMLLWPDFIAYNPKNGKNETFPAPAYACGLRAYID
                     HEQGWHKSLSNVPVKNVLGMSRHVFWSLQAEDSDANSLNNKEITTIIRRNGFRFWGNR
                     TPETNAYIFEVYTRTAQVLADSIAEAQFETIDSPLTPANVKDVISAIRAKLDSLVTAG
                     KLIGAECWYDVVDNSTTDLRQGRVRIRYKYTPVPPLEDMELYQTFTDEYFEPAFAVLG
                     GA"
     gene            6147..6657
                     /locus_tag="JQH65_001148"
                     /pseudo
     CDS             6147..6657
                     /locus_tag="JQH65_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
     gene            6713..7087
                     /locus_tag="JQH65_001149"
     CDS             6713..7087
                     /locus_tag="JQH65_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY6138631.1"
                     /translation="MKDIDTETRNNTVADNVTAVEDMAVERGVKLTRPIERGGEKITY
                     VEITGAIEQAGSLRGLSLSDVLNLKADTMFTLLPRVTSPRLDEVMIKKMSSRDFIQLC
                     AVAVNFMSEPDSGAKSVQETAA"
     gene            7096..7200
                     /locus_tag="JQH65_001150"
     CDS             7096..7200
                     /locus_tag="JQH65_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HAY6138632.1"
                     /translation="MCFEHIEDLVADIAAIFNWSPAEIFMMTPGEVVS"
     gene            7238..10045
                     /locus_tag="JQH65_001151"
     CDS             7238..10045
                     /locus_tag="JQH65_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAY6138633.1"
                     /translation="MKTLDIRVAFSAVDRLTRPAENARRLMGQFGDSIQRTQGAIKNL
                     ERQARSFERARDAVSKADAGIVKARRQLNALNQLQRTGTVLSEKQQKLMQQLSTRLER
                     LNESRTREIQKMRELGGELKRHGISLTGSDNTIQQAIRRTEQYNNQLERERQALARVT
                     RARERYSRAQETAGKLKTGGALAIGAAAAGGYAAGRFLQPAIGFGKEMSRVQALTRID
                     QNSPQFKALREQALKLGSETQFTAGDAASGQAFLAMAGFTPQAIQAALPGVLSMATAG
                     GMDLGETADIGSNILTQFGLSADQMDRVGDTLTAAFTRTNTDLRALGETMKYAGPVAG
                     KLGISLEQAAAMAGVLANMGIRGSDAGTAMRASLARLASPPKAAAEALKELGVSVSDA
                     GGKMRPMEDVLADLYKATRKYGEVDRVSFFKDIAGEEAFTSFMALVDAAGDGSLPKLR
                     KELEGARGEAERTAKVMANNLDGDLKSLSSAWEGLRIRIADLIDGPLRSVTQWLTRVV
                     SKVTALAQAHPALTRQLLIAGGALLAMTATVGSLSLAIGVLAGPLAKLRLGFSLLTGS
                     MNAVRVLPALWGMVTGSVSLLGGAIGALFSPVGLIVAALAGAAVLIWKYWDPIRAFFA
                     GVFSGIMERLTPLRETFERFGPVFDAIGSGISQVFNWFKSLLSPMESSKETLDKCTSA
                     GEIFGNVLGGALQLVLTPAKMLLDTLAWILEKLGVLPDEAERARKKIEDAQRAAILQD
                     KVALLQGDLAKINPPKPVENGNGTGGDNPKDNKPLTDSNTGTLRRLSKIADNTGKLVE
                     ETKKRIGPGDIVFKNLPRALAVRGEWQERKIAQVSKPAPAINITPVVPAPLPPALVPV
                     VAASSRPVAEAIRSPVASVPVTSRNREPVAFGFGGEIHVHLHNVVTQNPRELAKLVGE
                     MVRAEMERRARAGRGCFYDKD"
     gene            10058..10546
                     /locus_tag="JQH65_001152"
     CDS             10058..10546
                     /locus_tag="JQH65_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY6138634.1"
                     /translation="MMMIYGMFVFELRTLPHQQLQQNKSWRHVKNERVNRSASWQYIG
                     AGDDRIVLSGVLYPEITGGEVSLSLLTTQAYTGRPWPLIDGVGQIYGMYVLTETNTTR
                     SEFDRYGKAKKIEFSLTLERCDEDLRERLQSSSFSDMLSGFKDKVTSSLNSAASSVKG
                     LF"
     gene            complement(10575..11174)
                     /locus_tag="JQH65_001153"
     CDS             complement(10575..11174)
                     /locus_tag="JQH65_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY6138635.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALVCAGCEQIFEDKLSGTKTDRPG
                     LKRALKRLQKGDTLVVWKLDRLGRSMKHLISLVGELRERGINFRSLTDSIDTSSPMGR
                     FFFHVMGALAEMERELIVERTLAGLAAARARGRTGGRRPKLTKEQHEQIARLIKNGHD
                     RKQLAIIYGIGTSTIYRYHPVGDIQTEETTGQTQENENR"
     gene            11953..12486
                     /locus_tag="JQH65_001154"
     CDS             11953..12486
                     /locus_tag="JQH65_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY6138636.1"
                     /translation="MMHLKNITAGNPKTKEQYQLTKQFNIKWLYSDDGKNWYEEQKNF
                     QPDTLKMVYYHNGVIICIEKDVSAINPEGASVVELPDITANRRADISGKWLFKDGVVI
                     KRTYTEEEQRQQAENEKQSLLQLVRDKTQLWDSQLRLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(12515..13078)
                     /locus_tag="JQH65_001155"
     CDS             complement(12515..13078)
                     /locus_tag="JQH65_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000930007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY6138637.1"
                     /translation="MAHGITSHRCKLCSILKNIKSGNPKTKEQYQLTKNFDVIWLWSE
                     DGKNWYEEVNNFQDDTIKIVYDENNIIVAITKDAPTLNPEGFSVVEIPDITANRRADD
                     SGKWMFKDGTVVKRIYTADEQQQQAESQKVALLSEAESVIQPLERAVRLNMAMDEERT
                     RLESWERYSVLVSRVDTANPEWPQKPE"
     gene            complement(13047..15197)
                     /locus_tag="JQH65_001156"
     CDS             complement(13047..15197)
                     /locus_tag="JQH65_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY6138638.1"
                     /translation="MSTTTRKFKTIITDTGAKKLAQVAAPDGKPVRLTHMAVGDGGGT
                     LPTPDSKQTRLVHEVWRHTVNRVILDATHQNRIIAELVIPPETGGFWIREIGVFDEHG
                     DLIAVGNTAESYKPAVAEGSGRAQTFRTILTVSSTATVALTVDNTMVMATVDYVDDKL
                     KEHEQSRRHPDASLTAKGFVQLSSATNSVSETQAATPKAVKAAYDLANGKYTAQDAST
                     TRKGLVQLSSATNSTSETQAATPKAVKAAYDLANAKYTAQDATTAQKGIVQLSSATNS
                     TSETLAATSKAVKAVMDETNKKAPLNSPALTGTPTTPAARQGTNNTQIASTAFVMAAI
                     AALVDSSPDALNTLNELAAALGNAPNFATTMTNALAGKQPKDATLTALAGLATAADRF
                     PYFTGNDVASLATLTKVGRDILAKSTVAAVIEYLGLQETVNRAGNAVQKNGDTLSGGL
                     TFENDSILAWIRNTDWAKIGFKNDADGDTDSYMWFETGDNGNEYFKWRSRQSTTTKDL
                     MTLKWDALNILVNAVINGSLGVGSTNALGGSSIVLGDNDTGFKQNGDGILDVYANSQR
                     VFRFQNGVAIAFKNIQAGDGKKFTLSSSNNSTKNATFNLWGASTRPVVAELGNEAGWH
                     FYSQRNTDNSVIFSVNGQIQPSNWGNFDSRYVKDVRLGTRVVQLMARGGRYEKAGHAI
                     TGLRIIGEVDGDDEAIFRPIQKYINGTWYNVAQV"
     gene            complement(15200..15730)
                     /locus_tag="JQH65_001157"
     CDS             complement(15200..15730)
                     /locus_tag="JQH65_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HAY6138639.1"
                     /translation="MSNSLLPPSASNFMRCAEAVGTRITDIPVDLNTLWSPDTCPVHL
                     LPYLAWAFSVDRWDRNWPEETKRQVIRDAWLIHRHKGTISALRRAIEPLGYLIRVSEW
                     WEFGGEPGTFTVEVGTLDSGVTEEMYLEMERLIADARPVSRHMTGLNIIQEIPGDIFA
                     AAATYDGEVITIYPGD"
     gene            complement(15723..16619)
                     /locus_tag="JQH65_001158"
     CDS             complement(15723..16619)
                     /locus_tag="JQH65_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HAY6138640.1"
                     /translation="MPAVDLSQLPEPAIIAEPDFEAILADTKAMMIASYPAEQREAVS
                     AALELESEPLNVIAQTMSFREMLLRQRVNEGARACMLSHSAGTDLDNLAGNMNTKRLV
                     ITPATDTTDAVMESDTSLRLRAQRAYDGLSVAGPSGAYEYFARSASGLVRDARAISPS
                     PANVTVSILSTEGDGTATEALLNTVRAVLNAEDTRPVADRLTVQSARIVTWRLNAKLY
                     FYPGPESEPILAAAESSFRKWLAEQGLIGQDVALSAIAAALHVHGVQRVEIIEPTQNM
                     AISDIQAARCESFTISEGGRNE"
     gene            complement(16623..16973)
                     /locus_tag="JQH65_001159"
     CDS             complement(16623..16973)
                     /locus_tag="JQH65_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HAY6138641.1"
                     /translation="MTTRYTGMNPDGTGNLNDMEHLKQSVRDILTTPLASRVMRREYG
                     SLVPDLIDEPMNNTTRLQCMSAAVIALTRWEPRIALDAIDVVWKAGGRAGVTLSGTVM
                     QTMQNVELTITLRE"
     gene            complement(16970..17551)
                     /locus_tag="JQH65_001160"
     CDS             complement(16970..17551)
                     /locus_tag="JQH65_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HAY6138642.1"
                     /translation="MSAELMRLLSNIIRTGIISEVDEKSWRVRVRSGELETGWLRWNT
                     SRAGAFNVWLPPSPGEQVVIACIGGNPETAMIIGSLWSDAIPAPGKSLKEIVVSAPDG
                     AVFRYDADAGALSASGMKTATLQASVSVTLDTPVVECTDLLRTATLDVTKGGKMSGNI
                     THSGGNFTSNGITVHTHKHGGVKGGSDSTGGPQ"
     gene            complement(17548..18183)
                     /locus_tag="JQH65_001161"
     CDS             complement(17548..18183)
                     /locus_tag="JQH65_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HAY6138643.1"
                     /translation="MDKAFTRVDETFEAIRDSLNQQAINNIARKLAQDLRRAQQARIR
                     PQKAPDGTAWTPRRRHVTRIQERIRFIWNNEARTLKNWHHDTGKYGRTITGWDEDKNS
                     IRTFYRDDIDRFLEIRTRRINQDSTKRVPMFVKLRTARYLKARADASGVTVGYSGVAA
                     RIARVHQFGERDQVAPGIFTDYPVRELLGISQADERLIYNTVLGRIAEAVR"
     gene            complement(18176..18643)
                     /locus_tag="JQH65_001162"
     CDS             complement(18176..18643)
                     /locus_tag="JQH65_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY6138644.1"
                     /translation="MLKTDSLREAMTRSCRWCQANPEKFTIFVESGNIETTGETPSFV
                     YRYQMVMFVMDYAGELDDLTLPLLAWLSENQPQLLLNPERNQDIKFSAVINDDDSADL
                     LFTLPLRERVRITRSSQGTPQAEHLLEPKPRLPSSEGDWSHVFQDVTWGESDG"
     gene            complement(18781..19188)
                     /locus_tag="JQH65_001163"
     CDS             complement(18781..19188)
                     /locus_tag="JQH65_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HAY6138645.1"
                     /translation="MKLWPTLGVAFLLTAGWGTSMRLSWSLGRENARNEAQASTLKST
                     ADTLNIISAGVQDMQQVLAQLRVENQQRNQDGEVRREQLRNDIAKDECAHALPDARFT
                     DRLRRHAERATASAVSPAYTADADHTGNASPLP"
     gene            complement(19185..19577)
                     /locus_tag="JQH65_001164"
     CDS             complement(19185..19577)
                     /locus_tag="JQH65_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAY6138646.1"
                     /translation="MSNTFKFSSRSEKNLQGVNPDLVKVTRRALEISEVDFGITEGLR
                     SRYRQKQLVATGKSQTMNSRHLTGHAVDVVAYIGSQMSWEWPLYEKIAAAFRQASREL
                     NIPVEWGGDWKTLKDGPHFQLPHGAYPA"
     gene            complement(19574..19897)
                     /locus_tag="JQH65_001165"
     CDS             complement(19574..19897)
                     /locus_tag="JQH65_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAY6138647.1"
                     /translation="MSTIQTGITEQVIAWLFDHLPTVYAVGAAVSISALMSLYDGRTL
                     VQTVTGSLACGVLAMAVAGSLRFFGFPEDAVTFIGASIGFMGAEKARDKVIAAFNRRV
                     KEKDE"
     gene            complement(19900..20100)
                     /locus_tag="JQH65_001166"
     CDS             complement(19900..20100)
                     /locus_tag="JQH65_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY6138648.1"
                     /translation="MKVKALEGDTVDSLCFRYYGTTQGVTEKVLDANPGLCQQVFLDA
                     GQEVEMPEPEKKKREMIQLWGE"
     gene            complement(20100..20594)
                     /locus_tag="JQH65_001167"
     CDS             complement(20100..20594)
                     /locus_tag="JQH65_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HAY6138649.1"
                     /translation="MTIVARTEPGPAEDDITDTDDGDTRISAGAFWPDIVLRELRLAV
                     RLPGRVTTSRLLHTATGAVAHVTRELEAWQQEQQAAGHQTLADVPAPVINGESVNLWH
                     WRNAVYTATRALILERYRDADTTDKGDRRADALDIQTSDLWRDVSWAISDILCRPRIF
                     AELC"
     gene            complement(20698..21498)
                     /locus_tag="JQH65_001168"
     CDS             complement(20698..21498)
                     /locus_tag="JQH65_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAY6138650.1"
                     /translation="MITPAQQHWQNVMAQRAGRANEGVDHAARTAHEEVLYRLRLAQA
                     RLKGVQARSAKAAIKKELLPDFSGWIEGTLEADGGQQDEVIATLMVWAIDCGDLPLAL
                     RIGAYVVRHNLIMPDNFGRTAATVLTEEICNPVLTQAGTDADADLSAFIEPLDTLREI
                     VTDQDMPDEVRAKLCKACAFARRGLSDADSMALSLKLLREAMHLNPNAGVKREIATLS
                     RALKKADSAAEPEDASAQQAQDESSKSKKTTRKPATRKTTATQKAKRG"
     gene            complement(21544..22416)
                     /locus_tag="JQH65_001169"
     CDS             complement(21544..22416)
                     /locus_tag="JQH65_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005136493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P2 family phage major capsid protein"
                     /protein_id="HAY6138651.1"
                     /translation="MIGVTDQKGEKILLDTTGPIARTNTSYDGTKRRNPNNVVDLKNR
                     KYQCEQVNYDTFISYPQLDAWSAPPDFQSRISAQIARQVALDRIMIGFNGTSHADESN
                     FSTNKLLQDVNVGWLEHIRTDASERVMNDVTLTSRNMDNTVAHAGKYANADALVQDAR
                     SSLLDEWHKEADDLVVIMGRNLFNSLRLPVLNSISGQNPNAELLAGQLILSSRTIGGL
                     GVFLAPFFPDATMLITSFNNLSIYWQKGSMRRLMKDEPEYNRIATYQSINDAYVVEDY
                     GKCAMVTGLKFADS"
     gene            complement(22413..22595)
                     /locus_tag="JQH65_001170"
     CDS             complement(22413..22595)
                     /locus_tag="JQH65_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005054714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HAY6138652.1"
                     /translation="MNLVMSDITRNKLGCYMAQQVSLNNIPVSALVSRFTVEPAVQQR
                     FENASKESTEFTKKIT"
     gene            complement(22619..23455)
                     /locus_tag="JQH65_001171"
     CDS             complement(22619..23455)
                     /locus_tag="JQH65_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HAY6138653.1"
                     /translation="MANEKKTSRKKFRVAVSGSTVDGREISPVHLREAAENFNPDVYA
                     ARVNVEHYLSPCPSSEFSAMGDVTALSTEEITEGPLAGRTALYAEIEPTERMKQLVAD
                     GKKIYSSIELHPQFSVNGRAYLVGLAMTDTPASLGTERLKFTAQQRQAVMTFNSIQGE
                     APLISEAIESEIIEMAEQRQEEGTQWFNRVMGIIGRGRKADDASFSRIQEAVEGVATS
                     QADIIDRFNVLETRHQQDRQKITSLTTELAALKEKLRTQDGDPQNRFTATGAASDQLA
                     DF"
     gene            23610..25361
                     /locus_tag="JQH65_001172"
     CDS             23610..25361
                     /locus_tag="JQH65_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY6138654.1"
                     /translation="MIQDAFVRQRARQLYWQGYPPAEISRLMGINPNTIYAWKKRDQW
                     DETPPGQRVTQSIDARLIQLTEKQNKTGGDFKEIDLLTRQLKKLHDGQPDVMAAGKKG
                     RAKKLKNHFTPEQIAALREKIISRLEWHQRGWFDSLTLCREAGIRNRMILKSRQIGAT
                     WYFAQEALLMALRDDVAQPYQRNQIFLSASRRQAFQFKSIIQKAAAEVDVELKGGDKI
                     ILSNGAELHFLGTSAASAQSYTGNFYFDEFFWVSRFAELRKVAGAMATLSGLRRTYFS
                     TPSTETHEAYAYWNGDRWNEKKASHKRQRFSVDWKTLHNGLICPDRTWRQIVTLEDVV
                     NHGWKHTDIDEIRDENTEDEFLNLYMCEFVREGESAFNLNILIGCGVDGYDDWKDWKP
                     FAPRPMGNRPVWIGYDANGSSGNGDSGAVSVVVPPSVPGGRFRTVETRRVQGLEFEEQ
                     ARVIEEFTCRYNVEHIGIDVTGGNGEAVYQIVKRFFPAAIPYTFTLSSKRSLVLKMLQ
                     IMRAGRWEYDRAERELVAAFNAVRKVKTPGGFITYETDRARGISHGDLAWATMLAVIN
                     EPIGGEGENERFTVMEF"
     gene            25361..26407
                     /locus_tag="JQH65_001173"
     CDS             25361..26407
                     /locus_tag="JQH65_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAY6138655.1"
                     /translation="MSRKNKKVRMSSRIDLADALRKESSLSAFTFDGPYRLTGHDLLD
                     NMYCADNGRWYETPVDWYGLARAARQTSWHQSALYFKRNVLLGCYIPHPLLSRQDFSA
                     LALDWFVFGNAFLELRSNMLGEPLKLRHALAKYMRRGSDLESWRYVQDGKDAFQFRPG
                     KVCHLMNPDINQEIYGMPEYLGALLSASLSHSADMFRKLYYDNGSHAGCIIYIGAAQV
                     NRESMDSLKETLQGARGGGAFKNVLIHAPNGGKEGVQILPFQQITAKDEFMNVKAASR
                     DDVLAAHRVPPQLMGAMPGEKSAFGDVEKAARVYAINELMPVMEAMKHINDWLGEEVI
                     RFNPYALLDIQPTS"
     gene            complement(26898..27488)
                     /locus_tag="JQH65_001174"
     CDS             complement(26898..27488)
                     /locus_tag="JQH65_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HAY6138656.1"
                     /translation="MSEINYQALRERYSPVPVPKCPICGEEMSIQRISGAQVVYACSG
                     YGDDGDFKIGRTLADEHYEKSRVTVLDVGDPEVLALLDWLETKDNRIAELEKIATDYA
                     LKFQKAQDALKHAALLHSRTAQQTNNFAVSLPDISEYFINDVFQPLRYERDVERAIIK
                     AGGKALWQEKHEDRTHQSCDVNCGWFSPLTTDKNNT"
     gene            complement(27552..27863)
                     /locus_tag="JQH65_001175"
     CDS             complement(27552..27863)
                     /locus_tag="JQH65_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012907691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein"
                     /protein_id="HAY6138657.1"
                     /translation="MTTPTRRISFYLKPTAVKNEGEACAWLDSLTPEARKSGQRVAFL
                     AGLALLKMNPAEAYRLAAWAGDEALSVTQTRTERPASQPVSTAQITSQMAGNIRALFP
                     E"
     gene            complement(27868..>28471)
                     /locus_tag="JQH65_001176"
     CDS             complement(27868..>28471)
                     /locus_tag="JQH65_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="stability/partitioning protein"
                     /protein_id="HAY6138658.1"
                     /translation="RKKANVMRPVEYQNGEAFTIRNVQVMPESIPAGFKALADMSPFE
                     SLLIVDLGGTTLDVAKVQGQLAGISQVFCDPHVGVSLMADAVLSVMATNGMRTSHHIA
                     NTIIEHRHDEAWLRQHIHNDAHYASLMAVIREKEETLKQRVIRALAVFSGYGRVMVVG
                     GGAEIVAPAIREACGVNATFIADGVPQFALVNGLYAMDKE"
BASE COUNT         6531 a         7168 c         7405 g         7367 t
ORIGIN      
        1 ggatgccctc tgatgagtga atgggggagg cgtgctttga agtgaatttg tggaaggctt
       61 ccagtgttga gaaacatacg ccgcattcta gattgttaca ctgataatac ttttgtctca
      121 cggtgtttga atcattttcc ggacgactgg tgcggatacg ggcagatgcg ccacaaagcg
      181 gacaacggaa catagcgacc tctcttattg tagtgcttac actattctag gttgtattct
      241 ctaagattta aacatgactt tctctatata atgattttta gcattagtta ataaacgaac
      301 ggcactctaa ggagcgccgt caaagatatt attctgatgt cactctttgc gagtgagcta
      361 tttttaaatc agcgtttcct tcaaatacta tattcaactc atagttaaga gttagcttga
      421 atttatctga gtcacttttg atattctctg aagtgacatt tactaactta ttattatcat
      481 attctctaat aattaattta gtgaagtagt tatcctttac cacaagagaa aatggttgat
      541 taacaaatgg taagaagtaa accttacggg taccggattc agtagttatg ttcatagagc
      601 catcatcatt ttttctcatt agttctctga ggttgggatt atcgaaaagc atacgattat
      661 attgctgaaa gatctcttta ctcatcacaa gaaccagttt gccagccaag tcagcagatg
      721 attttatgtg atcaatcaac atattgaaat cttctattac ggtatgttgc gaacagccag
      781 tgctaaataa atttgaaagt attattctct ggatgtcctt gagaattaag tctgcttgaa
      841 tatcaccttc attgatataa gggatggatt ctattccttc ggcatacggt tctttatcta
      901 tcttaataga tatcttatgt tttgtgaaac aatcgctagc ctgcttaaaa tcaatagatt
      961 tgaaaagtga aagagggagc gttttcttga tgcgtttacg aagatgttct gatgttttac
     1021 gtgcagtgct atctagtcga tgtacatcta tggcagcatc ctgaagtctc atatcaagat
     1081 ctttctttag catgttaata tgctcttgaa gcaactttct taagttttct ttttttgttt
     1141 ggagatcgtc caatgttaaa gtcagcttga ttttagtgta ttcgatggag ttacaaattt
     1201 ttgataatac tccaatgagt cgttgcttct cacgatatgt tatatagttg ttttctattg
     1261 cttcttttat ttcttttgat acgctatcgt tctttttaga aaaaattaat aatagctgaa
     1321 cccatgattg ttccatatca ataaatccac agtcaaaaac attactgtaa actctgccca
     1381 tcaccatgtc tgttttatca tagtcacgta aacgccttgc taaactattc atccacccat
     1441 tgtgctccat attttcagac catgtccaca ggcagattct aattaatatc ttgattagtt
     1501 ggcttgcatt atcaatttct tcagtgggag tttcatatcc accggtttgg tcaataagtt
     1561 taccctctaa aataagttta atgtaatgtg tcattagttt gtcagaagta ttgattcttg
     1621 atgtcagata aagacatgtg catatacgca tgtcagtcaa cgcatttatg atggcaatat
     1681 tgaaccactc tctgtctgaa ttgaagttta gttttttgga tatgaaaaaa cccgggttaa
     1741 aaagctggcc tttgtaactg tatttttcta ccgaatggct gtcaatattc attgagtgct
     1801 gccatcggtt aataagatcg acggctgtat caatagtaga aggatcgcga gtctgtgttg
     1861 catcaataat catcgatgga agttcttgta gttgcgtttt gcaaaataat tcaaataaat
     1921 ctgaattctc tgtattgcag aaacgaaagt ttatgctttc ttgatattgc tcccaaacag
     1981 tgatataggt tcttaatata cgattataac gtgaacgaag cgaggcattt aagagtgatt
     2041 ggttgtttcg gaaccattct gttaatattg accattgtga acgagtaatt cccatataga
     2101 tacaaagttc attgctgata agatgttttc tattattgat aatacgcact ccgctccaaa
     2161 gacataattc aaaaaatctt tcagaaagat taattttatc catggaatta tttgatattt
     2221 tataaacttc atctgaaaac tcatattgaa aactccgttc aaataattct gtagtgatca
     2281 gtaagaagtt attgttggta ttatcatctt gaaaaaaata caaatcgcag agattattaa
     2341 agttattaat taatgcagag atagcattgt caaaactaat gagatcagaa tcccgcagat
     2401 acatatacgt atctgctgtt atagcctgca ttgtaagact tacggaaaaa tctcctcctt
     2461 taatcggagc cctataaaaa cattgcttaa gtatttttat taataagctg tctggtttga
     2521 tatttttaac gctaaaaagt atcttacccg tggattctgt ttcttttgta cgattatcac
     2581 caatgacaaa tgacgcaaaa tgattcttct tttttagttg gtaattaata agattaagag
     2641 taagttgaaa aggacggtga tataaactta actcatcttc cttagaataa ctgctagcca
     2701 cagatatgta ttctcctttg taatcggctt gcgtgatttc taaatttgaa tagttatgtt
     2761 tttgatagat agggtataac ctaagcttgg ctgagatttt gttacaaata tggggcatta
     2821 ctatttcgaa ggcaatgtat cgctttataa ttatttgccg ttttacatca tcaagaattt
     2881 caagactcac gataaagaac caaatagaaa gaactatgtt tatcaaaagc cataatatgc
     2941 tgattagaca agctatacca taaagataac ttccaaagac agttttaatt agtacactga
     3001 gaagtataaa tcctgataag aaaagcccgc tgagtccagc aagcatgaat cctgagtaac
     3061 gctgataagc agtttgggct atttttctat ctgccttttt tggaataaga cacttactaa
     3121 acctataaca agggggtata caataccaat aattgtcaat tggccactta aaatggttgt
     3181 ttgccattcg gttaatgaat gcctaaacgg gaaataatgt aaaagattat tttttataaa
     3241 taattcgttt aaatatccaa cataaagtag tacaaatgaa aaagctgata tgcaaagtac
     3301 tgatttaacc ggtgaggatg ttaatcttat gatgtttttt tcaataaaat tccattttaa
     3361 attcgaataa aatggatttt taaccatttt attacgaaca tagttctttg ctaaaatctc
     3421 tgttgcactt aaaaattgtc caatcatgaa ttactcttct ataaagtgtg ggtctactaa
     3481 ctaaactgga acttttacta ttgagtcata ttcccttgga cgggatggac tgtgaagatt
     3541 atacttgact tcctctgtca tgtgggaaaa acatccgtat tattgttagc atctggttga
     3601 tgccccaacc tctgacatct agtgggctga tacattatct tttgttctta ctgtcagtgt
     3661 agaagcacat ctcaaaaata atatttttaa gagatttatt ctgtttctgc tatccattcc
     3721 gggatttttg cttcaagttc aagctgcgtg gtaaagccgc tgttatcaat ggtgtgctcg
     3781 gcttttgcaa tagtccagtc ctgataatca atctcgcttt taaagcctgt cactgtgcca
     3841 tgcatttcgg gatagagttc tgcacgacca cgcgccagcg tgatggagaa tgatgcggct
     3901 ccgcgttgta gctgctgcca ctttgccgcc gctgcgcgtc ttgctgcctg ctcgttctga
     3961 taagtcttgc gtaacacaaa cacgttgcct tccgcacctt ccatatagtc gccttcccga
     4021 ctgctgcttt tctccttttt gggttttggc ggtttgcggc gtttcacgct gacttttttc
     4081 tttttcccgt aattaagatc aagccagtag gcgcgtaccc ccgtatacgc ctcgcggtca
     4141 gcaatgcgga actgatggcg atcgccgctg ctgcgtgtaa tggcgaacga gggcaacggc
     4201 tgcccctgtg cgttcacgcc accacctggc atgatgaata acagattgcc gctttttacc
     4261 gtggcgattg ctcccagcat ttccgccatg cgcgtaagaa aggacatgtc gctttcttcg
     4321 gtctggtcgg cgtggtcgat ttcgatatcc atcagcattt cgctgatttg cggtttcaga
     4381 ccgtaccgat gagtgatggc ggataccaca cgctcaacgg tcacatcatg ccaggacacc
     4441 tcacgtttaa cgttaaattc atcccgaaaa tctgcgcttc tggctgaaac agtcagcctg
     4501 tccggcggtc cttcgtgagc gatttcatca acaatgtaag tgcctttttc tgtcagcggt
     4561 tcccctttcc agccaatgag aaccgtcaga cgcgcgcccc gtggcggtag ctgcaactga
     4621 ccatccgcat catccagcgt gatggtgagc tggtccgcct caaatccccg gttgtcggtc
     4681 agttacaggc tcatcaggcg ctccgccacg cctgacagcg ttttaccctc cgagagaata
     4741 tcaaaatccg gcattttcac gggggccgtg ccctgactga gcaattgcat ggtggtgtcg
     4801 gtcatctgct ccctccctgt gcggcatggt cgcatgtgcg tgcggagggg gttactgctt
     4861 tttgttgtcg ccgtggcggg agaacggcgc aggggtgaga ttacgcgcgt ggtgggtgat
     4921 gattgttgct gaatcattta acggatacaa ggggctgaag ctatgagtga aactcgtttt
     4981 catggtgccc gtgttacgga gaataccgac ctggtaacag cgattaacga tgttgattcc
     5041 agcgttatcg gtatcgtggc aacggcggat gatgcggacg cgaagctgtt cccgctgaac
     5101 aagcccacac tgctgacccg cgtcaatgac gtgctgggaa aatgcgggac aacggggacg
     5161 ctttatcgtg cgcttaaggc catcgcagac caggtgagca caaaggtgat cgtcgttcgc
     5221 gtggctgaac acaaagaaga agacggaaag acgcaggatc aactggttat cggtggttct
     5281 gaggatgacg gcagctatac ggggatgtat gcgctgcttg ttgcagagca ggatgaaagc
     5341 atcggatacc gtccgcgtat tctggccgcg ccggagctgg acacggaggc ggtaacaaaa
     5401 tccctgtgcg tgattgcaga taaactgcgc gcgtttgtgt atgcctcatg tcatggctgt
     5461 aacacgatgg ccgaggcaat tacctaccgc cagaaattca acgaacgtga ggtgatgctc
     5521 ttatggccgg acttcatcgc ctacaacccg aaaaatggca aaaacgaaac gttccccgcg
     5581 cctgcctatg cgtgcggcct tcgtgcgtac attgaccatg aacagggatg gcacaaatcg
     5641 ctgtccaacg ttccggttaa aaatgtgctg gggatgtcga ggcatgtgtt ctggtcgttg
     5701 caggccgaag acagcgatgc caacagcctc aacaacaaag aaatcacgac cattattcgt
     5761 cgcaacgggt tccgcttctg gggtaaccgc acaccggaaa cgaacgccta catctttgag
     5821 gtgtataccc gaaccgcaca ggtgctggct gattcaattg cggaagcgca gtttgaaacc
     5881 atcgacagtc cactgacgcc tgcgaatgtg aaggatgtta tcagtgccat cagggcaaaa
     5941 ctggattcac tggtgactgc cgggaaactg attggcgcgg agtgctggta tgacgtggtg
     6001 gataacagca ccacggattt acgtcaggga cgtgtgcgta ttcgctacaa atatacgccc
     6061 gttccgccac tggaagacat ggagctttac cagacgttta ctgatgaata ctttgaaccc
     6121 gcatttgcgg tgctgggagg tgcctgatgg ctgtgccaaa acatcttcgc ttttttacgc
     6181 tgtttgtgga tggtgaaaac gaagtgggta atgtgacgtc cgtcactctg cctaagctga
     6241 cgcgcaaaac cgacagctac cggggtggtg gcatgatggg tgcggtaagt attgatctcg
     6301 gtctggacga ctccgcgctt gatgcgagct ttgtcatggg ggcgcagttc gtgagctgtt
     6361 ccttaagtat ggcggcacga ttgacggcac gctgctgcgt tttgcgggtg aatactacac
     6421 cgatgcagaa agcgacctgt atgaagtcga aatgcgcgga cgtgtgacgg aaattgatat
     6481 gggggaagcc aaacagggcg aagccacatc acacacttac gccattaaaa cacctactac
     6541 aagctgagtg ttaacgatcg cccgttgtgg gagattgacc tgctgaactt catttaccgg
     6601 aaggacggca aggacattgt gcccgatcgc atccgttccg cgcttgggct tggctgataa
     6661 gtaatatgct ggcggcgcag tgcgtcgcct ctgactgaaa aggagacaac agatgaaaga
     6721 catcgatact gaaacccgga ataacaccgt ggcggataat gtgacggcag ttgaggatat
     6781 ggctgtcgaa cgtggcgtaa aactgacccg accaattgag cgtggtggcg aaaaaatcac
     6841 gtatgtggag atcaccgggg ctattgagca ggctggatcc ctgcgtggtc tgtcgctgtc
     6901 tgatgtgctg aatctgaaag cggataccat gttcacgctg ttgcctcgcg tgacctcgcc
     6961 acgactggat gaagtgatga ttaaaaaaat gtcgtcacgc gattttattc agttgtgcgc
     7021 tgtggctgta aattttatga gcgagccaga ctctggcgcg aagagcgtgc aggagacggc
     7081 agcgtaatca ccctggtgtg ctttgagcac atcgaagatc tggtggcgga tattgccgcc
     7141 atttttaact ggtcgcccgc cgaaatcttc atgatgacgc ccggcgaagt ggttagctga
     7201 cgtgagcggg cggcacttcg tagcgggaat gcagacaatg aagactcttg atatccgggt
     7261 cgctttcagc gccgttgaca ggctgacccg gcctgccgaa aacgcccgcc gcctgatggg
     7321 gcagtttggt gactccatcc agcgaacgca gggggcgatc aaaaatctcg agcgtcaggc
     7381 gcgatcattt gagcgcgccc gtgacgctgt cagtaaagcg gatgctggca tcgtgaaagc
     7441 acgacgccag cttaacgccc ttaatcagtt acaacgcacg ggtacagtgc tcagcgaaaa
     7501 acaacaaaag ctgatgcagc agttaagcac ccggcttgaa cgcctgaatg aatcgcgcac
     7561 acgggaaatt cagaaaatgc gggagcttgg cggagagctg aaacgccacg gcatttccct
     7621 gacaggcagc gataacacca tccagcaggc catcagacgc accgaacagt acaacaacca
     7681 gcttgaacgc gaacggcagg cgcttgcgcg tgtaacacgg gcgcgtgagc ggtattcgcg
     7741 cgcgcaggaa acagcgggaa aactgaaaac aggtggtgcg ctggcaattg gtgcggcagc
     7801 ggcgggcggc tatgctgccg ggcgtttttt gcagcctgcg atcgggttcg gcaaagagat
     7861 gtcccgcgtt caggcactga cgcgaatcga ccagaacagc ccgcagttta aggcgctgcg
     7921 tgagcaggcg ttaaaacttg gctctgaaac gcagttcacc gcaggcgatg ccgccagtgg
     7981 acaggcattt cttgcaatgg ctggctttac accacaggcc attcaggctg cgctccctgg
     8041 cgtactgagc atggcaacgg ctggcggtat ggacctcggt gagacggcgg atattggctc
     8101 aaatattctg acgcagttcg gtctttctgc tgaccagatg gaccgggtcg gcgacacact
     8161 caccgcagcg tttacccgta ccaacactga ccttcgcgca ctgggtgaaa ccatgaaata
     8221 tgcaggtccg gtggcgggta agctgggaat atctctggag caggccgcag cgatggcggg
     8281 cgtgctggcg aatatgggta tcagggggag tgatgctggt acggcaatgc gtgccagcct
     8341 ggctcgtctg gcatcaccgc caaaggcggc agcagaggcg ctgaaagagc ttggcgtgtc
     8401 tgtctcggat gccgggggca aaatgcgccc gatggaggat gtgctggctg acctttataa
     8461 agctacccgc aaatacgggg aagttgaccg ggtatcgttc tttaaggaca ttgccggaga
     8521 agaggctttc acatcattta tggccctcgt tgatgcggca ggtgacggct ccttacccaa
     8581 actgagaaaa gaacttgaag gcgcgcgcgg tgaggctgaa cgcacggcaa aggttatggc
     8641 caacaacctt gacggcgatc tgaaatcact cagcagtgca tgggaagggt tgcgcatccg
     8701 cattgcagat ctgattgacg gtccgctgcg ttctgtcacg cagtggctca cgcgtgtggt
     8761 atcaaaggtg acggcgctgg cgcaggccca tcccgcactg acgcgccagc tactgattgc
     8821 aggcggtgca ttgctggcaa tgactgcaac ggttggctcg ttgtcgctgg ctattggtgt
     8881 gcttgctggc ccgctggcaa aactgcgtct tggtttttcc ctcctgaccg gatcaatgaa
     8941 tgctgtcagg gttctgccag cactatgggg aatggtgacg ggttccgttt ctttactggg
     9001 aggcgctatc ggggcgttgt tcagtccggt tggtcttatc gtggctgcgc ttgccggagc
     9061 tgccgttctt atctggaaat actgggatcc catcagggca ttttttgccg gggtgttcag
     9121 cgggattatg gaaaggctga ccccgttgcg cgaaaccttt gaacggtttg gtcctgtttt
     9181 tgacgcaatc gggagtggga tcagccaggt gtttaactgg tttaaatcgc tgctgtcacc
     9241 gatggagtcc agcaaggaaa ctctggataa atgtaccagt gctggcgaga tattcggtaa
     9301 cgttcttggc ggtgcgttac aacttgttct gacacctgca aaaatgttgc tggatacgct
     9361 ggcgtggata cttgaaaagc ttggtgtgct tccggatgaa gcggaaaggg cgcgcaagaa
     9421 aatcgaagac gcacagcgtg cggccattct tcaggacaag gttgccttgc ttcaggggga
     9481 tctggcgaaa atcaatccgc cgaagcctgt ggaaaatggc aatggcaccg gaggtgataa
     9541 ccccaaagac aataaaccgc tcacagacag caataccggt acgctgcgca gactcagcaa
     9601 aattgctgat aacacaggta agctggttga ggagacgaaa aaacgcattg gccccggcga
     9661 tattgtcttt aaaaacctgc cccgagcact tgctgttcgt ggggagtggc aggagcggaa
     9721 gattgcgcag gtcagtaagc ctgcccccgc aattaatatc acacccgtgg ttccggctcc
     9781 gctgcctccg gcgctggtcc ctgttgttgc ggccagctcc cgcccggtgg cggaggctat
     9841 acgatctcca gtggcatcag ttcctgtaac ttcccgtaac cgggagcctg ttgccttcgg
     9901 atttggtggt gaaattcatg ttcatctgca taacgttgtt acgcagaatc cccgcgaact
     9961 ggcgaaactg gtcggtgaaa tggtcagggc agaaatggaa cggcgcgccc gtgccgggcg
    10021 tggctgtttt tacgataaag attgaggagt catggccatg atgatgatct acggcatgtt
    10081 tgtttttgag ctgcgcacgc tgccgcatca gcagttacag caaaacaaaa gctggcggca
    10141 tgtgaaaaat gaacgcgtta accgttcagc aagctggcag tatatcggtg caggtgatga
    10201 tcgcatcgtt ctttctggtg tgctttatcc tgaaattaca ggtggcgaag tgtcgctgtc
    10261 gctgctgact acgcaggcgt atacaggacg accctggcct ttgattgatg gcgtcgggca
    10321 gatttacggc atgtatgtcc tgactgaaac gaatacgacc cgctccgagt ttgatcgcta
    10381 cggtaaggcg aaaaagatag aattttcact gactcttgaa cgctgtgatg aggatttgcg
    10441 ggagcgcctg caatcctcat cgttcagtga tatgctgtcc ggcttcaaag ataaggtgac
    10501 atcatctctt aacagcgcgg ccagttcagt taaagggctg ttctgattta acacaaaaac
    10561 cgctaatggt cagattagcg gttttcattt tcctgagtct gcccggttgt ttcttcagtc
    10621 tgtatatcgc ctacagggtg ataacgataa atcgtcgatg tgccaatgcc gtaaattatt
    10681 gccagttgtt ttctgtcgtg cccgtttttg attagccttg ctatttgctc atgctgttct
    10741 tttgtcagct tcggtcgacg tccgcctgtg cgcccccttg cgcgcgctgc cgccagcccg
    10801 gccagtgtac gttcaacaat taattcacgt tccatttcag ccagggcacc catcacgtgg
    10861 aagaaaaaac gccccattgg agaagatgtg tctatgctgt cggtcagact gcgaaaatta
    10921 atccctcgct cccgtagttc cccgacgaga gaaatcagat gtttcatgct tcgcccgagg
    10981 cgatccagtt tccagacaac cagcgtgtca cctttttgaa ggcgctttaa agcgcgtttt
    11041 aatcccggtc ggtctgtctt tgtcccgctt aatttatctt caaatatttg ttcacatcct
    11101 gcacaaacaa gagcgtttcg ttgcaggtct gtattctggt catttgttga tacccttaca
    11161 tagccaatca gcacactgaa tctcccgtcc aaaagcgcaa atcatgccat gcaggccaga
    11221 aacggccatt atttaaaacc tcggtttacg agaactcggc acaagcgggg agaaaatacc
    11281 gttactcagt acagcgaata cctggaccaa tcgacaaaca ttcagcggtg gcctttctgg
    11341 ggaactgtcc ggcaatgccg ctactgcaac aaagctgaaa acagcacgaa aaattgctgg
    11401 agttggtttt gatggttcta gcgatatctc aattagtgcc aaaaatgtca atgcatttgc
    11461 actccgacaa acaggtaata ctgttaatgg tgatacatcc gttggatgga attgggatag
    11521 tggtgcatat aacgccctga ttggtggtgc atctgcatta attcttcact ttaatataaa
    11581 tgctggtagc tgtcctgccg tacaattccg tgtgaattat aaaaatggtg gcatttccta
    11641 caggtcggct cgtgatggtt atggggttga attaggttgg tcagatttct ataccacgac
    11701 acgaaaacct tcagcgggag atgttggtgc atatacgcgg gcagaatgta actcaaggtt
    11761 tattacaggt attcgccttg gcggtctgtc atctgttcag acatggaatg gtcccggctg
    11821 gtctgacagg tcaggttatg tcgttacggg ttcagttaac ggaaaccgtg atgaattaat
    11881 tgatacaaca caggcaaggc caattcagta ttgcattaat gggacgtggt ataacgcggg
    11941 gagtatttaa cgatgatgca cttaaaaaac attactgctg gcaaccctaa aacaaaagag
    12001 caataccagc taacaaagca atttaacatc aaatggcttt attcagatga tggaaaaaac
    12061 tggtatgagg aacaaaagaa tttccagcca gacactttga aaatggtcta ttaccataac
    12121 ggcgttatta tttgtattga aaaggatgtt tcagcaatta atccggaagg cgcaagcgtc
    12181 gttgaattac ctgatattac agcaaatcgc cgggctgata tttcggggaa atggttgttc
    12241 aaagatggcg tagtgataaa gcgaacttat accgaggaag agcagaggca acaagcggaa
    12301 aatgaaaagc aaagcctgtt gcaacttgtc agggataaaa cccagctatg ggactcacag
    12361 ctacggctgg gcatcatttc cgacgagaat aaacaaaaat taaccgagtg gatgctctat
    12421 gcgcagaaag tcgaatctac agacacctcc agcctgccag taacgtttcc cgaacaacca
    12481 gaatgagaga aggcccgata ttgggcctta atttttactc aggcttttgt ggccattcgg
    12541 gatttgctgt atccacacgg ctgaccagaa cgctgtagcg ttcccatgac tccagtcgtg
    12601 tgcgttcctc atccatcgcc atattcagcc tgacagcgcg ttccagtggc tgaataacgc
    12661 tttcagcttc agaaagcaac gcgacctttt gtgattcggc ctgttgttgc tgttcgtctg
    12721 ctgtataaat ccgcttaacc acagttccgt ccttaaacat ccacttccct gaatcatcgg
    12781 cacgacgatt ggctgttata tctggaatct caacgacact aaagccttcg ggattaagcg
    12841 ttggggcatc tttggttatg gcaacaataa tattattttc gtcgtataca atctttatgg
    12901 tgtcgtcctg aaagttattt acttcctcat accagttttt accgtcttcg gaccataacc
    12961 agataacatc aaaattcttt gttagctgat attgttcttt tgtttttgga tttccggact
    13021 taatattttt taatatgctg cataatttac acctgtgcga cgttatacca tgtgccattg
    13081 atgtattttt gtattggtct gaagatggct tcatcatcgc catctacttc accaatgatt
    13141 cttaatccgg taattgcgtg tccggctttt tcataacgac caccacgcgc catcaattga
    13201 acaacacgcg tacccaggcg aacatctttt acatagcgtg aatcaaaatt cccccagttg
    13261 ctgggctgta tctgaccgtt aacagaaaat attaccgagt tatctgtatt tcgctggcta
    13321 tagaaatgcc atcctgcctc attgcctaac tctgcaacta ctggacgggt tgaagcaccc
    13381 cataaattaa acgttgcatt ctttgtggag ttgttggagc tggatagcgt gaatttttta
    13441 ccatccccgg cctgaatatt tttaaaagca atagccactc cattctgaaa gcggaataca
    13501 cgctgactat tagcataaac atccagaata ccgtctccat tctgtttaaa tccggtgtca
    13561 ttatcaccaa gaacaataga gctaccacct aacgcattcg tcgaaccaac tccaagactg
    13621 ccattaatga cagcattaac aagaatattt agtgcatccc atttcagcgt catcaggtct
    13681 tttgttgtgg tgctctggcg gcttctccat ttgaaatatt cattgccgtt gtcgcctgtt
    13741 tcaaaccaca tgtatgaatc agtgtcacca tcggcatcat ttttaaatcc aatcttcgcc
    13801 cagtcagtat ttcgaatcca ggcaaggatt gagtcatttt caaaagtaag cccaccggac
    13861 aaggtatcgc catttttttg cacggcgtta ccagccctgt ttaccgtttc ctgtaaaccg
    13921 aggtattcga taacggcagc aacggtcgat ttcgcaagaa tatcccgccc gacttttgtc
    13981 agggttgcca ggctggcgac atcattcccc gtaaaatacg gaaacctgtc tgccgcagta
    14041 gcaagcccgg ccagcgccgt cagggtggca tctttcggtt gcttacccgc aagcgcgtta
    14101 gtcatggtgg tcgcaaaatt cggggcgttt cccagcgccg ccgctaactc gttcagcgta
    14161 ttcagtgcgt caggtgacga atctacaagg gcggcaatcg cagccataac gaaagccgtg
    14221 cttgcgattt gggtattatt cgttccctgt cgcgcagctg gcgttgttgg cgttccggtc
    14281 agcgcaggac tgtttaaggg cgctttcttg ttcgtttcat ccattaccgc cttaaccgct
    14341 ttcgatgtcg cggccagtgt ttcagacgtg ctgttggtgg cactactgag ctggactatc
    14401 cctttttgtg ccgtcgtggc gtcctgagcg gtatattttg cgttagcaag gtcatacgcg
    14461 gcctttactg ctttcggcgt tgcagcctgc gtttcagacg tgctgttggt ggcgctactg
    14521 agctggacaa ggccttttcg cgtcgtgctg gcatcctgcg cagtatattt accgttagca
    14581 agatcatacg cggcctttac tgctttcggt gttgcagcct gcgtttcaga cacgctgtta
    14641 gtggcgctac tgagttgaac aaagcctttt gcggtcagcg aggcgtccgg gtgacgtcgt
    14701 gactgttcat gctctttcag tttgtcatcc acgtaatcca ctgtggccat caccatggtg
    14761 ttgtccacgg taagcgccac ggtggcagtg ctggatacgg tcagaatggt gcgaaatgtt
    14821 tgtgcacgac cggacccttc ggcaacggct ggcttgtaac tttcggcagt atttcccacc
    14881 gcgattaaat cgccgtgctc atcaaataca ccaatttccc ggatccagaa tccgcccgtt
    14941 tcaggaggaa taaccagctc cgcaataatg cggttctgat gtgttgcgtc caggatgacg
    15001 cgattaacag tatgtcgcca cacctcatgc accagacggg tctgcttact gtctggtgtg
    15061 ggcaacgtgc cgccaccgtc gcccacggcc atatgagtca ggcggacagg cttaccatct
    15121 ggcgcggcta cctgagctaa tttttttgca cccgtatcgg tgataatggt tttaaatttt
    15181 cgtgttgtgg tactcatgct taatcgcctg gataaatggt aataacttca ccgtcataag
    15241 ttgccgccgc tgcgaaaata tcccccggaa tttcctgaat gatattcagc cctgtcatgt
    15301 ggcggctgac cgggcgggca tcagcaatca accgctccat ttccagatac atttcctccg
    15361 tcacgccact gtccagcgtg ccgacttcaa cggtaaatgt tcccggttct ccgccgaact
    15421 cccaccactc agacacgcga atgaggtatc ccagcggctc aatggcccgg cgcagtgcgc
    15481 tgatggtccc tttgtgtcgg tgtatcagcc atgcatcacg aataacctgt cgctttgtct
    15541 cttccggcca gttgcgatcc cagcggtcaa cggaaaacgc ccaggcgaga taaggcagca
    15601 gatgcaccgg gcaggtgtcc ggcgaccaca gcgtgttgag gtctaccgga atgtctgtaa
    15661 tgcgtgttcc gacggcttcg gcacaacgca tgaaattgct ggctgatggt ggtaacaacg
    15721 aattactcat tgcgtccacc ttcgctgatg gtgaatgact cacagcgcgc cgcctgtatg
    15781 tcgctgatgg ccatattctg tgtgggttcg attatctcca cgcgttgcac accgtgcaca
    15841 tgcagtgcgg cagcaatggc ggacaacgcc acgtcctgac cgataagccc ctgctcagcc
    15901 agccacttcc tgaacgacga ttcagccgcg gccagaatag gttcggattc cgggccgggg
    15961 taaaagtaca gttttgcatt cagccgccat gtcacgattc tggcactctg tacggtcagg
    16021 cggtcggcca ccgggcgggt atcctctgca ttcagaacgg cgcgaacggt attaagcaac
    16081 gcctccgttg ctgtgccgtc gccctcagtg gacaggatgg aaaccgtcac gttggccgga
    16141 gacgggctga tggcccgcgc atcgcgtacc agaccgctgg cgctgcgtgc aaaatactcg
    16201 tatgcacctg acgggccagc aacactcaga ccgtcatatg cccgttgcgc ccgtagtctc
    16261 agcgaggtgt cactttccat cactgcatcg gtggtatccg ttgccggagt gataaccagg
    16321 cgctttgtgt tcatattgcc cgcgaggttg tccaggtctg tcccggcgct gtggcttagc
    16381 atgcaggcgc gtgcaccctc attgacccgc tggcgtaaca gcatttcacg aaacgacatg
    16441 gtttgagcga taacgttaag gggttccgat tccagctcca gcgcggcgga aacggcttca
    16501 cgctgttcgg cgggatagga cgcaatcatc atggcctttg tgtcagccag aattgcctca
    16561 aagtcaggct ccgcgatgat ggcgggttcc ggtaactgtg aaaggtcaac ggcaggcatg
    16621 atttactccc tcagcgtgat ggttaattca acattctgca tggtctgcat gacagtgccc
    16681 gacagcgtca ccccggcgcg gcctcctgcc ttccagacaa cgtcgatagc gtccagggca
    16741 atgcggggtt cccatcgtgt cagcgcaatc acggcagcac tcatgcattg cagacgcgtg
    16801 gtgttattca tgggttcgtc aatcaaatca ggcacaaggc tgccatattc ccgtcgcata
    16861 acccggcttg ccagcggggt ggtcaggatg tccctgactg actgtttcag gtgctccata
    16921 tcgttcaggt ttcccgttcc gtccgggttc attcctgtgt agcgggttgt cactgcgggc
    16981 ctcctgtcga atcgctgcca cctttaacgc caccgtgttt atgcgtatgc actgtgatgc
    17041 cgtttgaggt gaaattgccg ccgctgtgcg tgatattgcc gctcatcttt cccccttttg
    17101 tgacgtcaag cgtcgccgtt ctcagaaggt ctgtgcattc cacgacgggc gtatccagtg
    17161 tcacgctgac ggatgcctgc agggtggctg ttttcatgcc gctggcgctc agtgcgcctg
    17221 cgtccgcgtc gtagcggaac accgcgccat ccggcgcgct gaccacgatt tctttcaggc
    17281 ttttgccggg ggccggaatg gcatcactcc acaggctgcc aattatcatg gcggtttccg
    17341 ggttgccgcc aatgcaggca attaccacct gttcgcctgg tgatggcggc agccacacat
    17401 tgaaggctcc cgcgcgagag gtgttccagc gcagccaacc tgtttccagt tcgccgctgc
    17461 gaacgcgcac gcgccaggac ttctcatcaa cttcagagat gatcccggtg cggatgatat
    17521 tgctcagtag ccgcatgagt tctgcgctca ccgtacagcc tccgcaatcc ggcccagcac
    17581 cgtgttataa atcaggcgct catctgcctg gctgataccc agcagctcac gtaccgggta
    17641 atcggtgaaa atgcccggcg caacctgatc gcgctcaccg aactgatgaa cgcgtgcaat
    17701 acgtgcggcc acgccgctgt aacccaccgt cacaccggaa gcatctgcac gggctttcag
    17761 gtagcgggcg gtgcgcagtt ttacgaacat ggggacgcgc tttgtgctgt cctggttgat
    17821 gcgccgggtg cgtatttcca gaaagcggtc gatgtcatcc cggtaaaacg tgcggatgct
    17881 gtttttatcc tcatcccacc cggtgatggt tcgcccgtat ttccccgtgt cgtgatgcca
    17941 gtttttcagc gtgcgtgctt cgttattcca gataaagcga atgcgctcct gtatccgggt
    18001 tacgtggcgt ctgcgtggtg tccatgcggt cccgtccggc gctttctgtg gccggatacg
    18061 tgcctgctgg gcgcggcgta aatcctgtgc cagctttctg gcgatgttat tgatggcctg
    18121 ctgattcagg ctgtcgcgga tggcctcaaa ggtttcatcc acgcgagtga atgccttatc
    18181 catcgctttc accccacgtc acatcctgga atacatgcga ccagtcgcct tcggaagatg
    18241 gcaggcgggg ttttggctcc agcaggtgtt ctgcctgcgg tgtgccctga ctgctgcgcg
    18301 tgatgcgaac gcgttcccgc agggggagcg taaacaggag atcggcgctg tcatcgtcat
    18361 tgataacggc ggaaaatttg atgtcctgat tacgctcagg gttgagcaac aactgtggct
    18421 gattttcgga taaccacgcc agcagcggca gcgtgaggtc gtccagctcc ccggcgtaat
    18481 ccatgacaaa catcaccatc tgatagcggt aaacaaacga tggggtttct ccggtcgttt
    18541 caatgttgcc gctctccacg aaaatggtga atttttccgg gttagcctga caccatcggc
    18601 atgaacgggt catggcttca cgcagggaat cagttttcag catggttgtt atcctcgttg
    18661 ttcagtcgtt gcagcctgcg ctgttccagt aattcaatgg cccgtttatc cgcgttacag
    18721 gtttccagtg catccagaag gcggtcgccc catataccga gatttcccca tgtgggagtg
    18781 tcagggaagg ggggaggcgt taccggtatg gtcagcgtct gcggtataag ccggactgac
    18841 ggcgctggca gtggcgcgtt ctgcgtgcct gcgcagcctg tcagtaaaac gagcgtcagg
    18901 caaagcgtgg gcgcattcat cttttgcaat atcgttgcgt agctgttcac gtcttacctc
    18961 tccgtcctga ttgcgttgct gattttccac gcggagttgc gccagcacct gctgcatatc
    19021 ctgtaccccg gcgctgatga tattcagggt gtcggcggta cttttcaggg tgctggcctg
    19081 cgcttcgttt ctggcgttct cccggcccag cgaccacgac agacgcatgg atgttcccca
    19141 tccggcagtc agaaggaaag cgacgccaag cgtgggccag agcttcatgc cggataggct
    19201 ccgtgtggta actgaaaatg cggtccgtct ttcagggtct tccagtcgcc gccccattcc
    19261 accggaatat tcagttcccg gctggcctgt ctgaatgctg ctgcgatttt ttcgtacagc
    19321 ggccattccc atgacatctg gctgccgata taagccacaa catccacggc atgccccgta
    19381 aggtggcggc tgttcatggt ctggctctta cccgtggcca ccagttgctt ctggcggtaa
    19441 cggctgcgca acccttcggt gataccaaaa tccacttccg agatttccag tgcccgtcgg
    19501 gtcactttca ccagatcagg atttacaccc tgcaaattct tttcgctccg gctgctgaat
    19561 ttaaatgtgt tgctcattcg tccttctcct tcaccctgcg attaaaggcc gcaataacct
    19621 tgtcgcgtgc tttctctgca cccataaaac cgattgatgc gccgataaac gtcacggcat
    19681 cttcaggaaa cccgaagaag cgcaacgacc cggccacggc catggcaaga acgccgcacg
    19741 ccagcgatcc cgttacggtc tgaaccagtg ttcgtccgtc ataaaggctc atcagcgcgg
    19801 aaatgctgac cgccgcgcct actgcataca ccgttggcag gtggtcaaag agccacgcaa
    19861 taacctgctc tgtgatccct gtttgaatgg tgctcactgc tactcccccc acaactgaat
    19921 catttctcgt ttcttcttct ccggctccgg catctccact tcctgcccgg cgtccagaaa
    19981 tacctgctga cagagtccgg ggttggcatc cagcaccttt tcggtgacgc cctgcgtcgt
    20041 gccgtagtac cggaaacaga gcgaatccac ggtgtcgcct tccagtgcct tcactttcat
    20101 cagcacaact ccgcaaagat tcgcgggcgg cacagaatgt cagagatggc ccagctcaca
    20161 tcacgccaca aatccgatgt ctgtatatcc agagcgtctg cccggcggtc gcccttgtcc
    20221 gtagtgtctg catcacgata acgctccaga atcagggcgc gcgtggcggt ataaacagca
    20281 ttgcgccagt gccagagatt gacgctttct ccgttaatta cgggtgccgg aacatcggcc
    20341 agcgtctgat ggccagctgc ctgctgttcc tgctgccatg cttccagctc gcgggtaacg
    20401 tgtgccacag ccccggtggc agtatgcagc aggcgggagg tggtcacgcg ccccggcagt
    20461 cgtaccgcca gacgcagctc gcgcagcaca atatccggcc agaatgcacc cgctgaaatg
    20521 cgggtatcgc catcatcggt atcggtgatg tcgtcctctg cgggtccggg ttcagttctg
    20581 gcaactatag tcatggggtt cactcctgaa aaaaaatcgg gcggtgggtg cgcggtgtaa
    20641 acggtcacgg agtcaaactg gaacaccgcg cacgccgccc gctgacgggg tcagtcgtta
    20701 accgcgcttc gccttctgcg tcgcggtggt ttttcgtgtt gcaggcttcc gcgttgtctt
    20761 tttacttttg ctgctttcgt cctgcgcctg ctgtgcgctg gcgtcttctg gttcggctgc
    20821 ggaatcggct tttttcaggg cgcgggaaag ggttgcaatc tcgcgtttca cacctgcgtt
    20881 cgggttcagg tgcattgctt cgcgcagcag cttcagtgac agggccatgc tgtccgcatc
    20941 actcaggcca cggcgggcaa aggcgcacgc tttgcataat ttggcgcgca cttcgtccgg
    21001 catgtcctgg tcggtgacaa tctcccggag ggtgtccagt ggttcgataa aggcggacaa
    21061 atccgcgtcg gcatccgtcc cggcctgcgt cagtaccgga ttacagattt cttcggtcag
    21121 taccgtggca gcagtacggc caaagttatc cggcatgatg aggttgtgac ggactacata
    21181 cgcgccgata cgcagcgcaa gcggaagatc gccacagtca atcgcccaca ccatcagcgt
    21241 ggcaatcact tcatcctgct gcccgccgtc agcctccagc gttccctcaa tccagccgga
    21301 aaaatccggc aacaactctt ttttgatggc ggctttcgcg cttctggcct gtacgccctt
    21361 aagccgggcc tgtgccagac gcagacgata cagcacctct tcatgcgcgg tacgcgcggc
    21421 gtggtccacg ccttcattcg cccggcctgc gcgctgtgcc atcacgttct gccagtgttg
    21481 ttgtgcagga gtaatcattt ttgctctccg ttacaggcgg gcatgatgcc cgccgtgagt
    21541 tgattagctg tcggcgaact tcaggccagt aaccatcgcg cacttgccat agtcttcaac
    21601 gacataagcg tcattgatgg actggtaggt ggcgatgcgg ttgtattccg gctcgtcttt
    21661 catcaggcga cgcattgaac ctttctgcca gtaaatcgac aggttgttga acgaggtgat
    21721 cagcatcgtt gcatccggga agaacggcgc aaggaacacg cccagcccgc caatggtgcg
    21781 cgatgacagg atgagctgcc cggcgagtaa ttccgcattg ggattctggc cgctgatgct
    21841 gttcagcacg ggcagacgca gcgagttaaa caggttgcgc cccataatca ccacgaggtc
    21901 gtcagcttcc ttgtgccatt catccagcag ggatgaacgc gcgtcctgta ccagtgcatc
    21961 agcgttcgca tacttacccg cgtgcgccac ggtgttgtcc atgttgcggg aggtcagcgt
    22021 cacgtcattc ataacgcgtt cgctggcgtc ggttctgatg tgctccagcc agcccacgtt
    22081 aacgtcctga agcagcttgt tggtgctgaa gttggactca tccgcgtgag acgtgccgtt
    22141 gaaaccgatc atgatgcggt caagcgccac ctgtcgggca atctgtgcgc tgatgcggga
    22201 ctgaaaatca gggggtgccg accaggcatc aagctgcgga tacgaaataa acgtgtcgta
    22261 gttcacctgt tcacactgat atttgcggtt tttcagatca accacgttat tcgggttacg
    22321 gcgttttgtg ccgtcataac tggtattcgt gcgcgcaatc ggtcctgtgg tatccaggag
    22381 gattttttcg cctttctggt cggtcacgcc gatcacgtta tttttttcgt aaattcggta
    22441 ctttcctttg aggcgttttc aaaacgctgc tgcaccgcgg gttccacggt aaatcgcgac
    22501 accagtgcag ataccgggat attattaagc gacacctgct gcgccatata gcaacccagc
    22561 ttgttgcggg taatatctga catcaccaga ttcataaaaa atttgctcct ttgtctaatc
    22621 agaagtcggc cagctggtcg gaggctgcgc ccgttgcggt gaagcggttc tgcggatcgc
    22681 cgtcctgcgt gcgcagtttt tccttcagtg ctgccagctc tgtggtcagt gacgtaattt
    22741 tctggcggtc ctgctgatgg cgggtttcca gcacattaaa acggtcgata atgtcggcct
    22801 gtgacgttgc gacgccttcc accgcttcct gaatacggga aaaactggcg tcatccgctt
    22861 tgcggccacg gccaataatc cccattacgc ggttaaacca ctgggtgcct tcttcctggc
    22921 gttgttctgc catttcgatg atttcagact cgatggcttc ggagataagc ggcgcttcac
    22981 cctggatact gttaaacgtc atcaccgcct gacgttgctg tgccgtgaat ttcaggcgct
    23041 cagtgcccag gcttgccggg gtgtcggtca tcgccagccc gaccagatag gcgcgcccgt
    23101 taacggagaa ctgcgggtgc agttcgatac tggaatagat tttcttgccg tccgcaacaa
    23161 gctgcttcat gcgctcggtc ggttcgattt ctgcatacag cgcagtacgt ccggccagcg
    23221 gaccttccgt aatctcttcc gtactcagtg cggtgacatc gcccattgcg gaaaattcgc
    23281 ttgacgggca tggcgagaga tagtgctcaa cgttcacgcg ggcagcgtaa acatccgggt
    23341 tgaagttctc ggcggcttca cgcagatgca ccggactgat ttcacggcca tcaacagttg
    23401 atccggagac agccacgcga aactttttgc gggatgtctt tttttcatta gccatagttt
    23461 ttgcccctct gactggttct tcagtcatga tggcaaagcg taacaggctg atacaaaggg
    23521 cttttgttgt aagaaaacgg ccagaacaga gggttaagga gaacagtttc gcgcgcgggt
    23581 aatcttcctg taattactca gggggagcaa tgattcagga cgcttttgtg cgccagcgtg
    23641 cgcggcaact ttactggcag ggttatccgc ccgcagaaat atcacgtctg atgggaataa
    23701 acccgaacac gatttatgcg tggaaaaaac gtgaccagtg ggatgaaacg ccacccgggc
    23761 agcgtgtcac gcagtccatc gatgcgcgcc tcatccagct tactgaaaaa cagaataaaa
    23821 caggcggtga ctttaaggaa atagacctgc tgacccggca gcttaaaaag ctgcatgatg
    23881 gccagccgga tgtgatggcc gcaggaaaga aaggccgggc gaaaaaactc aaaaatcatt
    23941 tcacgccgga acagattgcc gcactgcggg aaaaaatcat cagcaggctg gagtggcatc
    24001 agcggggctg gtttgactcc ctgacccttt gcagggaagc cgggatacgt aacaggatga
    24061 tcctgaaatc ccgacagatt ggggcgacct ggtattttgc acaggaagca ctgctgatgg
    24121 cgctgcgtga cgatgtggcg caaccttacc agcgtaacca gatttttttg tctgcgtcgc
    24181 gtcgtcaggc gttccagttt aaaagcatta ttcagaaggc cgcggctgaa gttgatgtgg
    24241 agctgaaagg gggcgataaa atcatcctct ccaacggcgc agagctgcat tttctcggca
    24301 cttctgctgc gtcggcacag tcctatacgg gcaattttta ttttgatgaa tttttctggg
    24361 tcagtcgctt tgctgaactg cgcaaggtgg ctggcgctat ggcaaccctc agcggactgc
    24421 ggcgcaccta cttctccacg ccatccaccg aaacgcacga ggcatacgcc tactggaacg
    24481 gcgaccgctg gaacgagaaa aaggcctcgc ataaacgcca gcgtttttct gtggactgga
    24541 aaacgctgca taacgggctt atctgccctg accggacgtg gcggcaaatt gtcacgctgg
    24601 aagatgtggt taatcacggc tggaaacaca ccgatatcga cgaaattcgt gatgaaaaca
    24661 ccgaagacga gttcctcaat ctctatatgt gtgagtttgt ccgcgaaggg gaatcggcat
    24721 ttaacctgaa tatcctgatt ggctgcggcg ttgacggata cgacgactgg aaagactgga
    24781 aaccttttgc tccccgcccg atggggaatc gtccggtatg gattgggtat gacgcaaacg
    24841 gcagcagtgg caacggcgac agcggcgctg tgtccgtggt ggttcctccg tctgttcctg
    24901 gtggccgttt tcgaacggtg gagacgcgac gcgttcaggg gctggagttt gaagaacagg
    24961 ccagagtcat tgaagagttc acgtgtcgct acaacgtgga acacatcggc attgatgtga
    25021 cgggcgggaa cggggaggct gtttatcaga tagtgaaacg gtttttccct gccgctattc
    25081 cgtacacctt cacgctgtca tcaaaacggt cgctggtact gaaaatgctg caaataatgc
    25141 gtgccgggcg gtgggaatac gatcgcgccg aacgcgagct ggtcgcggcc tttaacgccg
    25201 tgcgtaaggt gaaaacaccg ggcggcttta tcacttacga aacggaccga gcgaggggga
    25261 tcagccacgg cgaccttgcg tgggcaacca tgcttgctgt cattaacgaa ccgattggcg
    25321 gcgaaggaga aaacgagcgt ttcacggtta tggagttctg atgagcagaa aaaataaaaa
    25381 agtgcgcatg agttcacgca ttgatctcgc tgatgcgctc aggaaagaat catcgctcag
    25441 tgcattcaca tttgatggtc cttatcgcct gaccgggcat gacctgctgg acaatatgta
    25501 ctgtgctgat aacgggcggt ggtatgaaac cccggtggac tggtacggtc tggcaagagc
    25561 tgcccggcaa acgtcctggc atcagtctgc gctttatttt aagcgcaatg tattgctcgg
    25621 ttgctacatc ccgcacccgc tgctttcccg gcaggatttc tcggcgctgg cgctggactg
    25681 gtttgtgttc ggtaacgcat tccttgagct tcgaagcaat atgctcggcg aaccgcttaa
    25741 attacggcac gccctggcga aatacatgcg acgcggaagc gatcttgaat catggaggta
    25801 tgtgcaggat ggcaaggatg cgtttcagtt tcgcccaggc aaagtgtgtc acctgatgaa
    25861 tcctgacatt aaccaggaaa tctacggcat gccggaatat ctcggcgcat tactctcggc
    25921 cagcctgtct cattcggcgg acatgttcag aaaactgtat tacgacaacg gatcccacgc
    25981 cgggtgcatc atctacatcg gtgccgcgca ggtaaaccgc gaaagcatgg actccctgaa
    26041 agaaacgcta cagggtgcgc gtggtggtgg tgcgtttaaa aacgtgctca ttcatgcgcc
    26101 caacgggggc aaagaggggg tgcaaatttt gccgttccag cagatcaccg caaaggatga
    26161 gttcatgaat gttaaggcgg catcccgtga tgatgtgctg gctgcgcacc gcgttccgcc
    26221 gcaactgatg ggggcgatgc cgggtgaaaa aagtgcgttt ggtgatgtgg agaaggccgc
    26281 gcgggtttac gcaattaacg agctgatgcc cgtcatggag gccatgaagc acatcaatga
    26341 ctggcttggc gaagaggtga tccgctttaa cccttacgca ctgttagaca tccagcccac
    26401 atcctgacgc gcttcgcttg tctgctgctt cgccggggca taaaaaattt atgccccgac
    26461 tctccagctc ctgtatcagt cagataattt cacgacgcct tccagtttat cgccaccatc
    26521 gacggtcaga ctcttacgca atcccaccgc gttgactgca tgttctcgcc gcctcagtgc
    26581 gattttgacg gccttacctt tcaccccatc aaatcagaat ccctcacgta tttttcacgc
    26641 tcagcgtgag aaatacggcc attctgtcgt gtcgctgcga catcgttaag ggaacgctat
    26701 ttaccccctg aaacgcgggc tgttcccccg tcacctgcgc gcagaaaaag cgcgtttttt
    26761 tgtgcacgca cggatccttg acggatccag ccgccacgcg ggctggaagg gcaaaaattc
    26821 gttcaaaaaa attgtgcaaa tttgtgcact gttgtgcagc agggcgatgt gcgattatcg
    26881 ccctggtttg agggggatca ggtgttattt ttatctgtcg tcagtgggct aaaccatcca
    26941 caatttacat cgcatgactg atgcgttctg tcctcgtgtt tctcctgcca caatgctttt
    27001 ccgccagcct ttatgatggc tctttcaaca tcccgctcgt atcgcaaggg ctgaaatacg
    27061 tcattaatga aatattcgct tatgtccgga agcgatactg caaaattatt tgtttgttgc
    27121 gccgtcctgc tatgcagcaa agcggcatgc tttaatgcgt cctgtgcttt ctggaattta
    27181 agtgcatagt cagtggcgat tttttccagt tcagcgattc ggttgtcttt ggtttccagc
    27241 caatcaagta gcgccaatac ttcaggatcc ccgacatcca acactgttac gcgtgatttt
    27301 tcataatgtt cgtcagcaag agttcggcca attttgaaat ctccatcatc accataaccg
    27361 gagcaggcat aaacaacctg tgctccagat attcgctgaa ttgacatttc ctcgccgcaa
    27421 atagggcatt tcggtactgg cacaggtgaa taacgttcac gtaatgcctg atagttgatt
    27481 tcgctcatta agtcgttctc cttgtttatg ttctgaggtg gagagcaggg cgaactcgcc
    27541 ctgatgttgt gttattcagg aaataacgcc cggatatttc cggccatctg actggttatc
    27601 tgtgcggttg atactggctg tgatgcgggg cgttctgtcc tggtttgtgt cactgataac
    27661 gcttcatcac cagcccatgc agccagtcga taagcctctg ccggattcat tttcagaagt
    27721 gccagcccgg ccagaaaagc cacgcgttgg ccgcttttgc gggcttctgg tgtaaggctg
    27781 tccagccagg cgcatgcttc tccttcgttc ttgacggcgg tgggcttcag atagaaactt
    27841 atccgtctgg ttggagtcgt cattggttta ctccttgtcc attgcgtaca gcccattaac
    27901 cagagcaaac tgtggcaccc cgtccgcgat gaaagtcgca ttaactccgc aggcttcgcg
    27961 gatagcgggt gccacaatct ccgcccctcc accgacaacc atcacccgcc cgtaacccga
    28021 aaaaaccgcc agcgcgcgga tcacgcgttg tttcagtgtt tcttcctttt cacgaattac
    28081 cgccatcagg ctggcgtaat gcgcgtcatt gtggatgtgc tggcgcagcc aggcttcatc
    28141 atggcgatgt tcgataatgg tattggcgat gtggtgactg gtgcgcatac cgttagtggc
    28201 catcaccgac agtacggcat cggccatcag agaaacgccc acgtgtggat cgcaaaacac
    28261 ctggctgata cctgccagtt gcccctgaac ctttgccaca tccagcgtgg ttccgcccaa
    28321 atccacaatc agcagggatt caaacggact catgtcagcc agtgccttaa agccagccgg
    28381 aatggattca ggcataacct gtacgttacg gatagtgaat gcttcgccgt tctggtactc
    28441 caccgggcgc atgacgttcg cttttttgcg g
//
LOCUS       DADLFA010000031        28420 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000031 DADLFA010000000
VERSION     DADLFA010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28420
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.031"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            79..1331
                     /locus_tag="JQH65_001177"
                     /pseudo
     CDS             79..1331
                     /locus_tag="JQH65_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            1728..2042
                     /locus_tag="JQH65_001178"
     CDS             1728..2042
                     /locus_tag="JQH65_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HAY6138659.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTTKAAPAKT
                     THHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQP
                     AA"
     gene            2135..2218
                     /locus_tag="JQH65_001179"
     CDS             2135..2218
                     /locus_tag="JQH65_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6138660.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            2318..3139
                     /locus_tag="JQH65_001180"
     CDS             2318..3139
                     /locus_tag="JQH65_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAY6138661.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTAQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVVLRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTNDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(3178..3507)
                     /gene="mdtI"
                     /locus_tag="JQH65_001181"
     CDS             complement(3178..3507)
                     /gene="mdtI"
                     /locus_tag="JQH65_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HAY6138662.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(3494..3859)
                     /gene="mdtJ"
                     /locus_tag="JQH65_001182"
     CDS             complement(3494..3859)
                     /gene="mdtJ"
                     /locus_tag="JQH65_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HAY6138663.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            4271..5305
                     /gene="tqsA"
                     /locus_tag="JQH65_001183"
     CDS             4271..5305
                     /gene="tqsA"
                     /locus_tag="JQH65_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HAY6138664.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMLSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(5330..6718)
                     /gene="pntB"
                     /locus_tag="JQH65_001184"
     CDS             complement(5330..6718)
                     /gene="pntB"
                     /locus_tag="JQH65_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HAY6138665.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQDVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(6729..8261)
                     /gene="pntA"
                     /locus_tag="JQH65_001185"
     CDS             complement(6729..8261)
                     /gene="pntA"
                     /locus_tag="JQH65_001185"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HAY6138666.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     TGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRRVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            8785..9729
                     /locus_tag="JQH65_001186"
     CDS             8785..9729
                     /locus_tag="JQH65_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY6138667.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGATSFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            9915..11297
                     /locus_tag="JQH65_001187"
     CDS             9915..11297
                     /locus_tag="JQH65_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY6138668.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLVLGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAASLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            11334..12056
                     /gene="folM"
                     /locus_tag="JQH65_001188"
     CDS             11334..12056
                     /gene="folM"
                     /locus_tag="JQH65_001188"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HAY6138669.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKLGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(12053..12376)
                     /locus_tag="JQH65_001189"
     CDS             complement(12053..12376)
                     /locus_tag="JQH65_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HAY6138670.1"
                     /translation="MKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHYIVAS
                     ERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWVLIIC
                     WIKLH"
     gene            12502..13221
                     /gene="rstA"
                     /locus_tag="JQH65_001190"
     CDS             12502..13221
                     /gene="rstA"
                     /locus_tag="JQH65_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HAY6138671.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGITICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     MPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRNVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            13225..14526
                     /gene="rstB"
                     /locus_tag="JQH65_001191"
     CDS             13225..14526
                     /gene="rstB"
                     /locus_tag="JQH65_001191"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HAY6138672.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLYERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLVQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            14602..15531
                     /gene="tus"
                     /locus_tag="JQH65_001192"
     CDS             14602..15531
                     /gene="tus"
                     /locus_tag="JQH65_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HAY6138673.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRSLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(15528..16931)
                     /gene="fumC"
                     /locus_tag="JQH65_001193"
     CDS             complement(15528..16931)
                     /gene="fumC"
                     /locus_tag="JQH65_001193"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HAY6138674.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(17074..18720)
                     /locus_tag="JQH65_001194"
     CDS             complement(17074..18720)
                     /locus_tag="JQH65_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HAY6138675.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            18919..20094
                     /gene="manA"
                     /locus_tag="JQH65_001195"
     CDS             18919..20094
                     /gene="manA"
                     /locus_tag="JQH65_001195"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HAY6138676.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            20195..21703
                     /locus_tag="JQH65_001196"
     CDS             20195..21703
                     /locus_tag="JQH65_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HAY6138677.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITVAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(21929..23194)
                     /gene="uidC"
                     /locus_tag="JQH65_001197"
     CDS             complement(21929..23194)
                     /gene="uidC"
                     /locus_tag="JQH65_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HAY6138678.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQRGLFDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPCENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIELWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGTGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(23233..24606)
                     /gene="uidB"
                     /locus_tag="JQH65_001198"
     CDS             complement(23233..24606)
                     /gene="uidB"
                     /locus_tag="JQH65_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HAY6138679.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVACVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYERYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARSGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            complement(24603..26403)
                     /gene="uidA"
                     /locus_tag="JQH65_001199"
                     /pseudo
     CDS             complement(24603..26403)
                     /gene="uidA"
                     /locus_tag="JQH65_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
     gene            complement(26791..27381)
                     /locus_tag="JQH65_001200"
     CDS             complement(26791..27381)
                     /locus_tag="JQH65_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY6138680.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(27610..28377)
                     /gene="hdhA"
                     /locus_tag="JQH65_001201"
     CDS             complement(27610..28377)
                     /gene="hdhA"
                     /locus_tag="JQH65_001201"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HAY6138681.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
BASE COUNT         7072 a         7327 c         6817 g         7204 t
ORIGIN      
        1 atatattaga cagaacaatt cgattttcct aacctatgta taagcctgat ccacaggcat
       61 atttagcaag gatttcaagt gagccgtact acaactgttg atggcgctcc ggcaagcgac
      121 actgacaagc aaagcatttc tcagccaaat caatttatta aacgcggtac gccgcaattt
      181 atgcgcgtca ccctggcgct gttctctgcc ggactggcaa cctttgcact cctctattgt
      241 gtgcagccta tccttccggt gctttcgcag gagtttggct taacccccgc gaacagtagt
      301 atttcactgt ccatttccac ggcgatgttg gctattggtt tgctgtttac tggcccccta
      361 tccgatgcca ttggtcgcaa accagtgatg gtcacggcgc tactgttggc ctccatttgt
      421 acgttatttc gacaataatg accagctggc acggcatttt gattatgcgc gccttgattg
      481 ggctttcgtt aagtggcgtg gcagctgttg gcatgactta tcttagcgag gaaatccacc
      541 ccagtttcgt ggccttttcg atggggttgt atatcagcgg caactcaatt ggcggcatga
      601 gcggacgctt aattagtggt gtcttcacgg actttttcaa ctggcgaatt gctctggcgg
      661 caatcggttg tttcgcgcta gcctcggcgt tgatgttctg gaaaatcctt ccggaatcac
      721 gccattttcg cccgacttcg ctgcgaccta agacgttgtt tatcaacttt cgtctgcact
      781 ggcgtgaccg gggattaccg ttattgttcg cagaaggctt tttgctgatg gggtcgttcg
      841 tcacgctgtt taattacatc ggctatcggt tgatgctttc cccctggcat gtcagtcagg
      901 ccgtggttgg cttattatcg ctggcttatt tgaccggtac acggagctca cccaaagccg
      961 gaaccatgac cacccgctac gggcgcggtc cggtgatgtt gttttcgacg ggggttatgc
     1021 tgtttggttt actgatgacc ttattcagct cgctgtggct gatctttgcc ggaatgttac
     1081 tcttctcagc aggattcttc gcagcccact cagttgccag cagctggatc ggcccccgcg
     1141 caaaacgcgc taaaggccag gcctcctcgc tgtatctgtt cagttactat ctggggtcga
     1201 gtattgccgg gacgctgggt gatgttttct ggcataacta tggctggaac ggcgtcggcg
     1261 catttattgc tctgatgctg gtcattgctc tgctggtcgg gacgcgtttg catcgtcgtc
     1321 tgcacgcctg aaaaataagc ccggactgcg gtaactacac gtccggactt attgcttgct
     1381 caaaccaacg ttaatagcca tcctaaaata gacgaagcgc cagccaattc ccgcagtgcg
     1441 tctagcgtca tcaggattat aagtacccaa ataaacggat tcattttgct gtgtgtcatt
     1501 tattactgat gcgcagttat tctaccgctt tgtaagtagt aaaatagtta acccgatcaa
     1561 gactactatt attggtagct aaatttccct taagtcacaa tacgttatta tcaatgctgt
     1621 aatttattca gcgtttgtac atatcgttac acgctgaaac caaccactca cggaagtctg
     1681 ccattcccag ggatatagtt atttcaacgg ccccgcagtg gggttaaatg aaaaaacaaa
     1741 ttgagggtat gacaatgaaa aaagtattag ctctggttgt tgccgctgct atgggtctgt
     1801 cttctgccgc ctttgctgca gagactgcga cgaccaccaa agcagcgccg gcgaaaacga
     1861 cacatcataa aaaacagcat aaagcagcac ctgcccagaa agcgcaggca gctaaaaagc
     1921 atcataaaaa tacgaaagct gaacagaaag cccctgaaca aaaagcgcag gcagcgaaga
     1981 aacacgccgg gaaacacagt catcagcaac cggcaaaacc tgctgcacaa cccgcagcgt
     2041 aagttttcaa cagtaatgct ggcgcgcccc ctcgcgcctg aaaattacgg tgctaagcgg
     2101 gtaacgttta gcaccgcctt tagcggaggg tagtatggtg ggccgttatc gctttgagtt
     2161 cattcttatc atccttattt tatgcgcact gattgccgcc cgtttttatc tttcctgact
     2221 gtagttatct gattttactc ccactttcat cccgtcccgt ctatagtatt tatgagggtt
     2281 tgcttttaat aatcataatt acccaccaga gtgtgatatg cgtacaacca ttgctgtagt
     2341 gttgggtgca attagtttga cgtctgcttt tgtgtttgca gataaaccag acgttgccaa
     2401 atcggcaaac gatgaggtca gcaccctgtt ttttggtcat gatgatcgtg tgccagtgaa
     2461 tgacacggcc caatcaccgt gggatgcggt tgggcaactg gaaacggcca gcggcaattt
     2521 atgtacggcg acgctgattg cacccaatct ggcattaacg gcaggacact gtttattgac
     2581 acctccaaag ggtaaagccg ataaagcagt ggtgctgcgt tttgtgtcaa ataaaggtct
     2641 ttggcgctat gagatccacg acatagaagg ccgcgttgat ccgacactgg gaaagcggtt
     2701 aaaagcagat ggggatggtt ggattgtacc tcccgcagcc gcgccgtggg acttcggatt
     2761 gattgtgcta cgtaatcccc cttctggcat tacaccgttg ccgttatttg agggagataa
     2821 agccgcgctt actgccgcat taaaatcggc tggccgtaaa gtaactcagg cgggataccc
     2881 tgaagatcat ctcgatacgt tgtacagtca tcaaaactgt gaagtgactg gctgggcgca
     2941 aacgtcggtg atgtcacatc agtgcgatac cttgccgggt gacagcggtt cgcctctgat
     3001 gttgcatacc aatgacggct ggcaattaat tggggtgcaa agttcggctc ctgccgcgaa
     3061 agatcgctgg cgcgccgata accgggccat ttctgttacc ggttttcgcg acaagctgga
     3121 tcaactgtcg caaaaataat gttcaaacgc tgcccgactg tgcgggcagc gtgttcatca
     3181 ggcaagtttc accatgatca ttccagccaa cagcaagacc aggccaatcc agcctttacg
     3241 atttaaccgt tgaccaaaca agatccaacc tgcggctaac gtggcggcaa taccaaaccc
     3301 gccccacaat gcataagcga cagacaagtc gatcccttta acggcctgag aaagcgcact
     3361 aaaggcagcc agcaccgccg ccagggagag caagccaaat attttgcgac gaaagccgtc
     3421 agaaaatttc aaaaagacgt tagcaacgat ttccagcacg attgccaatg ccagccaggc
     3481 ggcgtgaacc cattcaaact gcgccatggt ttacctccag ttcaggttta cgcgctttac
     3541 gggtacctga ttttatcaac acaatcccgg cgaccagggt ggttaacccg gcaattttca
     3601 tcagcgataa actttcgtcg aataacaaaa cgctaaacaa ggtaataaat aaaataccga
     3661 taccttccca cagcgcataa gctacgccta aggcgatctt tttaacggcg aaagagagaa
     3721 atatatacga cagagaaatc atcaccagca ttaaaataaa gccgccattt ccctcactga
     3781 cgctcgccca tttcattgat agcgtaccgg taatttctgt agcaatagcc aggcctaata
     3841 aaatccaata aatatacatt gtccttctcc tgcaagagaa ttattttaat tttcgcttaa
     3901 ttcagcgaaa ttaaagtaaa gtggtcaagc cccgcgcata gggcaagctt aggcagaaga
     3961 aaggactaaa gcgcgttgcg ccagtgctgc tcacctacga gcaagatagt ggatgaggta
     4021 caaagatggg taaatgttga aaataaggtc ctgaacaaat tgtccataat attacaatta
     4081 tccgcagtgt tgcttctcgt catcgcggat gataattgtc ctcggtagtt gaacacgcct
     4141 gatttgtatc atagcttaag aattaactca aaatattttc acttctttac ctgagcggtt
     4201 tgattttcgt tatgatgacg gagcgaaaaa gacattatta ttagcaaagg aagaaaaaac
     4261 ggggacaagc atggcaaagc cgatcatcac gctcaatggc ctaaaaatcg tcattatgtt
     4321 gggaatgctg gtcattattc tctgcggtat ccgttttgcc gccgagatca tcgtgccgtt
     4381 tattctcgca ttatttattg ctgttattct taacccgctg gtgcaacaca tggtccgctg
     4441 gcgagtgccg cgtgtactgg cggtgtcgat tttgatgacc atcatcgtga tggcgatggt
     4501 gttgctatta gcttatctgg gttccgcgct caacgagttg acgcggacgt taccgcaata
     4561 tcgcaactct attatgacgc cgctgcaagc tcttgagccg ttgttgcaac gcgtagggat
     4621 tgacgtctca gttgaccagc tggcgcatta cattgatccg aacgcagcga tgacgttgct
     4681 caccaactta ttgacgcagt tatctaatgc catgttatca atatttttat tgctgctgac
     4741 ggtgctgttt atgctgctcg aagtgccaca attgcccgga aaatttcagc aaatgatggc
     4801 gcgtccggtt gaagggatgg cggcgattca acgtgcaatt gacagtgttt ctcattatct
     4861 ggtgctgaaa acagccatca gcatcatcac cggcctggtc gcctgggcga tgctcgccgc
     4921 actcgatgtt cgcttcgctt ttgtctgggg attgctggcc tttgcgctta attacatccc
     4981 taatattggt tcagttctcg cggcaatccc ccctatcgct caggtactgg tgtttaatgg
     5041 cttctacgaa gcgttgctgg tgctggcggg atatctgctg attaatctgg tcttcggcaa
     5101 tattctggag ccgcgcatca tgggacgtgg gctggggctt tccacattgg tggtattttt
     5161 gtcgttgatt ttttggggat ggttgttagg accggtgggt atgctgcttt ccgtgccgtt
     5221 aacaattatt gtcaaaattg cgcttgaaca aacagcggga ggtcaaagca tcgccgttct
     5281 gttaagcgat ctcaataaag agtgacggcc tccgcagagg ccgtcagggt tacagagctt
     5341 tcaggattgc atccacgctg gctttggcgt caccaaacag catgtgggtg ttttccttga
     5401 agaacagcgg gttttgcaca ccagcatagc cagtgttcat cgaacgttta aagacaatca
     5461 cgtcctgcgc tttccacact tccagcacag gcataccagc aataggactc ttcggatcat
     5521 cctgcgccgc cgggttaacc gtatcgttag caccaatcac cagtacggta tcggtatcag
     5581 caaagtcatc gttgatctcg tccatttcca gcacgatgtc atacggtact tttgcttcag
     5641 ccagcaatac gttcatatgt ccaggcaaac gccccgcaac cgggtggata ccgaaacgca
     5701 cgttgatacc acgagcgcgc agtttctcgg taatttcagc gacaggatat tgcgcctgcg
     5761 cgactgccat gccgtacccc ggagtaatga tcactgaatg ggagtttttc agcagttccg
     5821 ctgtctcttc tgcggtgatt tcgcggtgct cgcccacttc ctgatcatcg ccagtagaag
     5881 agccgtcggt gccgaaacca cccgcaataa cgctgataaa ggaacggttc atcgccttac
     5941 acataatgta agaaaggata gcccccgaag aaccgaccag cgcaccggtc acaatcagca
     6001 ggtcgttgct gagcataaag cccgcagccg cagccgccca gccggagtac gagttcagca
     6061 tcgacaccac cactggcata tctgcaccac cgatggaggc gactaaatgc cagccgaata
     6121 ccagcgcaat ggcggtcatt attagcaatg ccagcacttg caggccgacg ctgtccgtgc
     6181 gaacaaatac aatcagcagc aggaaggaaa cgaccagagc cgccaggttc attttgtgac
     6241 ggtttggcag catcaacggt ttagacgaaa tcttgccaca cagtttgccg aacgccacca
     6301 ccgaacccgt gaacgttacc gccccgatga agataccgag gaacacttcc gtcaggtgaa
     6361 tattgaccag aatcggtgcc attcccgcgt catgatgcag atagctgtta aagccaacca
     6421 gcactgccgc cagacccacg aagctgtgca ggatcgccac cagttctggc atttcggtca
     6481 tttcaacttt cttcgccaga cggataccaa ttgccccacc aatgaccatc gccagcaaga
     6541 tccagccaac attacccgta tccggtccaa aaatggttgc gattaacgca atcgccatcc
     6601 ctgcgatacc gaagttgtta ccctggcgag acgtttcatg tttcgaaaga ccggccagac
     6661 tgaagataaa caggatcgcg gcaacaatgt atgcagctgt aactaatcct ccagacatat
     6721 gttacccctt aatttttgcg gaacattttc agcatgcgct gagtcacggt gaagccaccg
     6781 aaaatattaa tgctggctat aagcaccgcg ataaaactaa ggaagctaac ccagccgccc
     6841 tggccaatct gcaacagtgc tccgacaaca ataatccctg aaatcgcgtt ggtgaccgac
     6901 atcaacggtg tatgcagcgc gtgcgataca ttccacacca cgtaataacc gacaacgcag
     6961 gccagcgcga aaacggtgaa gtgcccaaga aactctttcg gcgcaacgct tgccagccaa
     7021 ccgaaaagga tgattgccag cgccatcaac gcgtatttac gccacggtga gcaggcacat
     7081 ttttcctcag ttttcacttc cggtgccgct ttttgtgccg cctgcggctg agctgatacc
     7141 tgaatcggcg gtgccggcca ggtaatttcg cccgcacgga tcacggtcac gcggcgaatc
     7201 accacatcat caaaatcaac agtgatattg ccgtctttct ctttgcacaa cagtttcagc
     7261 agattaacga ggttagtacc gtaaagctgc gaggattgcg tcggcagacg gcccggaaga
     7321 tcggtataac caatcacttt gacaccattt tccgtagtga agatttcacc cggcacggtg
     7381 tattcacagt tgccaccgtt ttgggctgcc aggtcgacaa tcacactgcc cgtcttcatg
     7441 gagtcaacca tttcacgggt aattagcttc ggcgctggtt tgcctggaat aagcgcggtg
     7501 gtgacaatga tatcgacctc ttttgcctgg gcggcaaaga gttccatttc tgctttaatg
     7561 aacgcgtccg acatcacttt ggcatagcca tcgccgctgc ccgcttcctc tttaaaatcc
     7621 agctcgagga attccgcgcc catactttga acttgttctt tcacttccgg acgggtgtcg
     7681 aatgcacgca caatcgcgcc gagactgttt gctgcgccaa tggcggccag acctgcaaca
     7741 cccgcaccaa tcaccatcac ttttgccggt ggcactttcc cggccgcagt aatttgcccg
     7801 gtaaagaagc gcccaaattc atgtgccgct tcaacaatgg cgcgataacc ggcgatgttc
     7861 gccatcgagc ttagtgcgtc cagcgattgt gcgcgtgaga tacgcggcac agaatccatc
     7921 gccatcacgg tcacgttacg ttccgcaagt ttttgcatta attccggatt ctgcgcaggc
     7981 cagataaaac tcaccagcgt tgtcccagga ttcagtaacg caatttcatc atctaacggc
     8041 gcattgacct tcagaatgat ctctgactgc cagacgctat tcccttctac aatttcagcg
     8101 cccgcttgca caaacgcttt atcgtcaaaa cttgccagtt gacccgcgcc gctctctacc
     8161 gcgacggtaa aacccagttt cagcagctgt tccactgttt ttggcgttgc tgcaacacgg
     8221 gtttcattgg ttaaccgttc tcttggtatg ccaattcgca tgatattccc ttccatcggt
     8281 tttattgatg atggtttgcc tgtgtcagga gccacacaag ccgctcatgt acgagctaaa
     8341 tgttactccg ttaaaataaa ttagtaacaa acgccctata acgtactgaa aattatacct
     8401 gtgatctagc gccaaaaatc agtatttcgg cgtgaactcg caaaatatta acgattcagc
     8461 cgtgatagtg ggataaacac cttagaacgc cggataaaga ctgataattg tcttcgacgg
     8521 tcgggtaaaa cgagacatcg ccccggcacg attcactact taacattaaa ttaacttata
     8581 caattcagtt gcttcagtag taatgatgct gatacggctg ttttttaagc atagacggtc
     8641 atttgagcag gattaaaatt ggcttaagga atgtgatatg aaaaatgacg cagacagtta
     8701 caccgtttaa atgcaataat cagccacgtt tctcgttaat aacaatacca gtacctggtt
     8761 tgcgcaaggc gaaggattat ttttatgaag cttaagaaca ccctcctggc gtcggcactg
     8821 ctttctgcta cggcattctc cgttaacgca gcaacagaac tgacaccgga gcaagcggca
     8881 gcggttaaac cttttgaccg tgtagtggtt accggtcgtt ttaatgctat tggcgaagcg
     8941 gtgaaagccg tttctcgtcg cgcagataaa gaaggtgcca cctcttttta tgttgtcgac
     9001 acttctgatt ttggtaacag cggtaactgg cgtgtggtcg ctgacctcta taaagccgat
     9061 gctgaaaaag cagaagaaac aagtaatcgc gtaattaacg gtgttgtcga actgccgaaa
     9121 gatcaggctg ttctgattga accgtttgac acggtcaccg tccagggctt ctatcgtagc
     9181 cagccagaag tcaatgatgc catcaccaaa gcggcaaaag cgaaaggtgc ctactctttc
     9241 tacatcgttc gtcaaatcga tgccaaccag ggcggcaacc agcgtattac tgcattcatc
     9301 tataaaaaag atgctaagaa acgtatcgtc cagagcccgg atgtgatccc ggcagattcc
     9361 gaagcaggac gtgcagctct ggctgccggt ggcgaagccg cgaagaaagt tgagatccca
     9421 ggtgttgcga ctaccgcatc accaagttct gaagtcggtc gtttctttga aacccagtca
     9481 tcaaaaggcg ggcgttacac cgtcacgctc ccggatggca ctaaagtcga agaactgaac
     9541 aaagcgaccg cagcgatgat ggtcccgttc gatagcatca aattctctgg caactacggc
     9601 aacatgaccg aagtctccta tcaggttgcg aaacgtgccg cgaagaaagg tgctaagtat
     9661 taccacatca cccgccagtg gcaggaacgt ggtaataacc tgaccgtcag cgcagatctg
     9721 tataaataac tattcagtta ctcctataag gcggcttgat gccgcctttc gcatttttag
     9781 tcacaaattt ccccgcgacc attgcatgca gacacatctc ttcgtaaaat cccgcgcttc
     9841 agtggcaaac tccattttta tgcgttttgc cctaatattt attcttctta tcacgtttta
     9901 atcactggat atcgatggaa aagaaactgg gactgagcgc actcaccgcg ctggtattaa
     9961 gctcaatgct gggcgcgggt gttttcagtc tgccgcaaaa tatggcggca gttgccagcc
    10021 cggcagcact gctcatcggc tggggtatta ctggcgctgg cattttattg ctggcctttg
    10081 ccatgctgat cctcacgcgc attcgtcccg aacttgacgg cggtatcttt acctatgccc
    10141 gcgaagggtt tggcgagcta atcggctttt gttccgcatg gggatactgg ctgtgcgcag
    10201 tcatcgccaa cgtctcctat ctggtgatcg ttttttccgc gttaagcttt tttacggaca
    10261 cgccggaatt gcgcctgttc ggcgatggta atacctggca atcgatcgtc ggtgcatcgg
    10321 ctttattgtg gattgttcac tttttgatcc tgcgcggggt gcaaaccgct gccagcatta
    10381 acctggtggc gacactggca aaattgttgc cgctgggtct gtttgttgtg ctggcgatga
    10441 tgatgttcaa actggatacc ttcaagctcg acttcaccgg acttgtactt ggcgtacccg
    10501 tttgggaaca ggtgaaaaac accatgctga tcaccctgtg ggttttcatt ggtgtggaag
    10561 gtgccgtggt tgtttctgcg cgtgcgcgta ataaacgtga tgttggcaaa gcgacactgc
    10621 tggcggttct ctccgctctg ggcgtttact tgctggtaac gctgctttcg ctgggtgtgg
    10681 tggcacgtcc tgaactggct gaaattcgta acccgtcaat ggccggtctg atggtggaaa
    10741 tgatgggacc atggggcgaa atcatcatcg ctgccagttt gatcgtttcc gtttgcggcg
    10801 cgtacttaag ctggaccatc atggcggcag aagttccgtt cctggctgcc actcataaag
    10861 cattcccacg catttttgcg cgccagaacg cacaagctgc gccatccgcc tcactgtggt
    10921 tgaccaacat ctgcgtgcaa atttgtctgg tactcatctg gctgaccggt tccgattaca
    10981 acacgttgct gaccatcgcc tcagaaatga ttctggtgcc ctatttcctg gtcggcgcgt
    11041 tcttgctgaa aatcgcaacc cgtccactgc ataaagcggt aggtgtcggt gcctgcattt
    11101 atggcttatg gttattgtac gcctctggtc caatgcactt gctgttatcc gttgttctgt
    11161 atgcgccggg gctgctggtt ttcctgtatg cgcgcaaaac gcatacacat gataatgtac
    11221 tgaaccgtca ggaaatggtg ctgattggca tgctgttaat tgcctcagtt ccggcgacct
    11281 ggatgctggt gggataacgt tcatcccatc gttggatagc aaaggagata acgatgggta
    11341 aagcccagcc cttgccaata ttaattactg gcggaggtcg tcgcatcggc ctcgccctcg
    11401 cctggcattt cattaatcaa aagcaaccgg tgattgtcag ctatcggacg cactatccag
    11461 ccattgatgg actgattaag gcaggtgcgc agtgtattca ggctgatttt tcgaccaacg
    11521 acggtgtgat ggcgtttgcc gatgaagtac taaaaagcac ccatggtctg cgtgctattt
    11581 tgcataacgc cagtgcgtgg atggcggaaa aactgggtgc gccactggcc gacgtactgg
    11641 cttgcatgat gcagatccac gttaataccc catacctgct caaccatgcg ctggaaagat
    11701 tactgcgtgg gcacggacac gccgccagcg atatcattca ctttaccgat tatgtggtgg
    11761 agcgcggtag cgacaagcat attgcgtatg ctgcaagcaa agcggcactg gataatatga
    11821 cccgctcgtt tgcccgcaag ctggcaccgg aagtgaaagt gaattctatt gctccatcgc
    11881 tgatcctgtt taatgaacat gatgatgccg aatatcgaca acaggcgctg aataaatcac
    11941 tgatgaaaac cgcgcctggc gagaaagaag tgatcgacct ggtcgattac ttacttacca
    12001 gttgctttgt caccggacgc agtttcccac ttgatggcgg tcgtcatctg cgttaatgca
    12061 gctttatcca gcatataatc aacacccatg cgcttatccc ccaacacgca acagaaccaa
    12121 caaaagcggc gggcaagcgc atcattccgg tgaaatacca cagcgacacc aggtaaacaa
    12181 aatagggaat aatcgaccac atactaaaaa tgatggttgc gcgtaaggct tcaatgccgc
    12241 gttcgctggc aacaatataa tgcgcgataa gcgcaaaggt cgggaaaagt ggaatcagcc
    12301 cggcgatata ataatttttc gtttttgcta aaacaccaat caacagcact accagcgcac
    12361 cgagcgcggc tttgatttta ccttaacact tccataacaa gtcatcagta gaatacctga
    12421 tgaaaacttg tttagaaacg attgatagta agtaaaaaca gcgcggtgta ttgtgacgtt
    12481 tttatatcta ccgtgaatgt tatgaacact atcgtatttg tggaagatga tgcggaagtc
    12541 ggttcactga ttgccgcgta cctggcaaaa catgatatgc aggttaccgt agagccgcgc
    12601 ggcgaccagg ccgaagaaac cattttgcga gaaaatccgg atttggtgtt actcgacatc
    12661 atgctaccag gcaaggacgg cattaccatt tgtcgtgatt tacgcgcaaa gtggtctgga
    12721 ccgattgttc ttctaacctc tctcgatagc gatatgaacc acatcctggc actggaaatg
    12781 ggtgcctgcg actatattct caaaacgatg ccccctgctg ttttgctagc gcgtttacgt
    12841 ttgcatttgc gtcagaatga gcaagccaca ctgaccaaag gtcttcagga aacgtctctg
    12901 actccctaca aagccctgca tttcggcacg ttgaccatcg atcccatcaa ccgcgtagtc
    12961 accctggcta acactgaaat ctcgctctcg acagctgatt tcgaattatt gtgggaatta
    13021 gctacccatg ccgggcaaat catggaccgc gatgcattgc tgaaaaattt acgcggcgtc
    13081 agttatgacg gactggatcg taacgtggac gtggctattt cgcggttaag aaaaaaactg
    13141 ctcgataacg ccgcagaacc ttatcgcatt aaaactgtgc gtaacaaagg ctatcttttt
    13201 gcgcctcatg catgggaata agcgatgaaa aaactgttta tccagtttta cctgttattg
    13261 tttgtctgct tccttgtgat gtctctgctg gttgggttgg tgtacaaatt taccgccgaa
    13321 cgcgcgggca aacagtcgct ggatgatttg atgaacagtt cgctgtatct gatgcgcagc
    13381 gaattgcgtg agatcccccc acacgactgg ggtaagacgc tgaaagagat ggatttaaat
    13441 ctctctttcg atctgcgtgt cgagccactg agtaaatacc atcttgatga tatttccatg
    13501 caccgactgc gtggcggcga aattgtcgcc ctggacgatc agtacacgtt tttgcagcgt
    13561 atcccgcgca gccactacgt gctggcagtt ggtcctgttc cttatcttta ttatctccat
    13621 cagatgcgat tgctggatat cgccctgatc gcttttattg ctatttccct cgcctttccg
    13681 gtgtttatct ggatgcgtcc gcactggcag gatatgttaa aactggaagc agcggcgcaa
    13741 cgatttggcg atgggcatct ctatgagcgt atccactttg atgagggttc gagctttgaa
    13801 cgacttggta tcgcatttaa ccagatggcg gacaatatca acgccttgat tgccagcaaa
    13861 aaacagctta ttgacggtat cgctcacgaa ctgcgaacac cgttagtgcg cctgcgttat
    13921 cgactggaga tgagcgataa cctgagcgcc gccgagtccc aggcgttgaa tcgtgatatc
    13981 agtcaacttg aagctttaat tgaagagctg ctgacttatg cccgactcga tcgcccacaa
    14041 aacgagcttc atcttagcga accagacctg ccgttgtggc tgtcaacgca tctggcagat
    14101 attcaggcag taacgcccga taaaacggta cggataaaaa cgctcgtgca aggccattat
    14161 gcggcgttgg atatgcgctt aatggagcgc gtgctggata atttgctcaa taacgccctg
    14221 cggtactgcc attcaacggt tgaaaccagc ctgctactgt cggggaatag agcgacatta
    14281 attgttgagg atgatggccc agggattgcc ccagaaaacc gcgaacatat ctttgaacct
    14341 tttgttcgcc tcgatcccag ccgggatcgc tcaaccggcg gctgcgggct ggggctggca
    14401 attgtccact ctatagcact ggcaatgggc ggtacggtta attgtgacac cagcgaactg
    14461 ggtggtgctc gcttctcgtt tagctggccg ttatggcata acatcccgca atttacctct
    14521 gcctgacact acgcgcacga tggtcaagtc accaccattg tgctataaat taaggatgtt
    14581 gtaactaaag tggttaatat tatggcgcgt tacgatctcg tagaccgact caacactacc
    14641 tttcgccaga tggaacaaga gctggctgca tttgccgctc atcttgagca acacaagcta
    14701 ttggttgccc gcgtgttctc tttgccggag gtaaaaaaag aggatgagca taatccgctt
    14761 aatcgtattg aggtgaaaca acatctcggc aacgacgcgc agtcgctggc gttgcgtcat
    14821 ttccgccatt tatttattca acaacagtcc gaaaatcgca gcagcaaagc cgctgtccgt
    14881 ctgcctggcg tgttgtgtta ccaggtcgat aacctttcgc aagcagcgtt ggtcagtcat
    14941 attcagcaca tcaataaact caagaccacg ttcgagcata tcgtcacggt tgagtcagaa
    15001 ctccccaccg cggcacgttt tgaatgggtg catcgtcatt tgccggggct gatcaccctt
    15061 aatgcttacc gctcgctcac cgttctgcac gaccccgcca ctttacgttt tggctgggct
    15121 aataaacata tcattaaaaa tttgcatcgt gatgaagtcc tggcacagct ggaaaaaagc
    15181 ctgaaatcac cacgcagtgt cgcaccgtgg acgcgcgagg agtggcaaag aaaactggag
    15241 cgagagtatc aggatatcgc tgccctgcca cagaacgcga agttaaaaat caaacgtccg
    15301 gtgaaggtgc agccgattgc ccgcgtctgg tacaaaggag atcaaaaaca agtgcaacac
    15361 gcctgcccta caccgctgat tgcactgatt aatcgggata atggtgcggg cgtgccggac
    15421 gttggtgagt tgttaaatta cgatgccgac aatgtgcagc accgttataa acctcaggcg
    15481 cagccgcttc gtttgatcat tccacggctg cacctgtatg ttgcagatta acgcccggct
    15541 ttcatactgc cgaccatctg ttctggccgt acccagctgt caaactcggc ttcgctaaga
    15601 taccccagcg caagggccgc agcttttaag gtcagccctt ctttatgcgc ttttttggcg
    15661 atctccgcgg ctttgtcata accaatgtgg gtgttaagcg cagtcaccag catcagcgat
    15721 tcattgagta attgattgat tcgctcacga ttcggttcaa tacccactgc gcagtgtttg
    15781 ttaaaacttt ccatgccatc tgccagcaag cgcaccgatt gcaggaaatt atggatcacc
    15841 atcggacgga agacgttcag ttcaaagtta ccggaagcgc cacccatgtt gatcgccacg
    15901 tcgttcccca ttacctgaca gcagagcatg gttaatgctt cacactgggt tgggttcact
    15961 ttccccggca tgattgagct gcccggctca ttttccggga ttgagatttc accaattccg
    16021 cagcgcgggc cagaggccag ccagcggaca tcattggcga ttttcatcag tgacgcagcc
    16081 aaccctttca acgcgccgtg cgcctgaacc agggcatcac aggtcgcaag cgcttcaaat
    16141 ttgttcggcg cggtaacaaa cggtgcacag gtaatgactg ccagttcatc tgctacgcga
    16201 cgcgcatact ccggatgggt atttagtcca gtacccaccg ctgtaccgcc aagagccagt
    16261 tccgctacgt gaggcaggct gtattcgata tgtttgagat tatgctcgag catcgctacc
    16321 cagccggaaa tctcctgccc tagtgttagc ggcgtggcgt cctgcaagtg ggttcgaccg
    16381 attttgacga tatcggcaaa tgcacgcgat ttttcactca gtgtctgcgt cagggtttta
    16441 agctgcggaa tgagttgctt gcgcagcgcc agcagcgccg caacgtgcat cgccgtcgga
    16501 aagacatcgt tggaactttg gcttttgttc acgtcgtcgt tagggtgaac tttacgttcc
    16561 atcccgcgca cgccgccgag taattcactg gcccggttag ccagcacttc gttcatgttc
    16621 atgttacttt gcgtgccgga gccggtctgc cagatagcca gcgggaattc gtcgtcatgc
    16681 tgtcctgcca gtacttcatc cgccgcctgc cgaatggcgc tcgctttctc ttcagacaac
    16741 aagcctaaat cttcattaac ttttgccgct gcgcgcttgg ttagcgccag cgcatgaatc
    16801 agtgaggtgg gcattttctc cgtcgaaatg cggaaatgct ccagcgagcg ttgagtttgt
    16861 gcgccccaca gcttatctgc cgggacatca atcgccccca tcgaatcttt ttcgctgcgt
    16921 actgtattca tggcctgctc ctcacctgtt taattttttc tttctgtttt gctttcgcta
    16981 agcaactttt agctcactta ttatttacta tttgataaca agtgtttggt ctttcgtgcc
    17041 atgtaaaaaa accgccccga agggcggctc tgtttatttc acacagcgag tgcactgtgt
    17101 gagttgtatc tgctggaaga agtcatttcc tttatcatcc acaaggataa acgccgggaa
    17161 atcttccact tcaattttcc agatggcttc cattcccagt tccggatatt caacacattc
    17221 caggctctta atacttccct gcgccaatac agcggccgga ccaccgatac tgccaaggta
    17281 gaagccgccg tgttttttac aggcatccgt cacctgctgg ctgcggttgc ctttcgccag
    17341 catgatcata cttccgccct gcgcttgcag ttgatcgaca taagaatcca tccgtccggc
    17401 ggtcgttggg ccaagagaac cggaggcata accttccggc gttttggccg gacccgcgta
    17461 gtaaatcgga tgatctttaa tgtactgcgg cagcccttca ccgttatcca tccgctcttt
    17521 cagtttggcg tgagcaatat cacgaccgac gataatcgtg ccgttaagcg ataagcgtgt
    17581 agaaacggga tactgcgaca actgtgcgag gatctctttc atcggacggt taaggtcaac
    17641 gcgcaccgct tcgccttctc ccgctttacg cagctcttca gggatatatt tacctggatt
    17701 atgttccagt ttttcgatcc agatcccctg acggttgatc ttcgctttga tattacggtc
    17761 agcagagcag gaaacaccca taccaaccgg acaggatgcg ccgtgacgtg gcaggcgaat
    17821 cacgcgtatg tcgtgagcga agtatttacc accaaactgc gcacccagac cgagattttg
    17881 cgcttcgatc agcaattctt tttccagttc cacatcgcgg aatgcctgac cgtgctcatt
    17941 cccttccgtt ggcagttcgt catagtattt agcggaagcc agtttcactg ttttaaggtt
    18001 agtttctgca gaagttccac caattacgaa cgcaatatga tacggaggac aggccgccgt
    18061 acccagcgtg cgcatcttct caaccaggta atttttcagt ttccccggcg ttagtaacgc
    18121 tttggtttcc tgatagagat acgtcttgtt tgccgaacca ccacctttgg cgatacagag
    18181 gaatttgtac tcgtcgccat caacggcata aagatcgatc tgcgctggca gattggtgcc
    18241 ggtattcacc tctttataca tatccagcgg cgcgttttgc gagtagcgca aattatcttc
    18301 gatataagtg ttatagacac cgcgcgccag cgccgcttca tcacagccac cggtccatac
    18361 acgctgccct tttttaccaa caataatcgc agtgccggta tcctgacagg ttggcagaac
    18421 acctttcgcc gcgatgtcgg agttgcgcag gaattgcagc gccacatatt tatcattttc
    18481 gctggcttcc gggtcacgca gaatgtcggc cacctgttgt tggtgagccg gacgcagcat
    18541 aaatgacgca tcatgaaacg cctgacgcgc caacagagtt aacgcttcgg gtgcgacttt
    18601 caaaatctcc tgcccttcaa attcagatac gctaacgtgt tcgctggtta gcaggtaata
    18661 ctcagtatca tcttttttga gtggaaaagg agcctgataa tgaaagggtt tgtttgacat
    18721 tgttctctca cttactgcct ggtttggtta tgctctgggc gggtgttccg ttgccctgtt
    18781 aaaagcgagt aacaatatcc tacacacttt tttaacaaaa actgagacta gtacgacttt
    18841 ttgcggctcc aggttacttc ccgtaggatt cttgctttaa tagtgggatt aatttccaca
    18901 ttaaaacagg gattgatcat gcaaaaactc attaactcag tgcaaaacta tgcctggggc
    18961 agcaaaacgg cgttgactga actttatggt atggaaaatc cgtccagcca gccgatggcc
    19021 gagctgtgga tgggcgcaca tccgaaaagc agttcacgag tgcagaatgc cgccggagat
    19081 atcgtttcac tgcgtgatgt gattgagagt gataaatcga ctctgctcgg agaggccgtt
    19141 gccaaacgct ttggcgaact gcctttcctg ttcaaagtat tatgcgcagc acagccgctc
    19201 tccattcagg ttcatccaaa caaacgcaat tctgaaatcg gttttgccaa agaaaatgcc
    19261 gcaggtatcc cgatggatgc cgccgagcgt aactataaag atcctaacca caagccggag
    19321 ctggtttttg cgctgacgcc tttccttgcg atgaacgcgt ttcgtgagtt ttccgagatt
    19381 gtctccctac tccagccagt cgcaggtgca catccggcaa ttgctcactt tttacaacag
    19441 cctaatgccg aacgtttaag cgaactgttc gccagcctgt tgaatatgca gggtgaagaa
    19501 aaatcccgcg cgctggcgat tttaaaatcg gcgctcgaca gtcagcaggg tgaaccgtgg
    19561 caaacgattc gtttaatttc tgaattttac ccggaagaca gcggcctgtt ctccccgctg
    19621 ttactgaatg tggtgaaatt gaatcctggc gaagcgatgt tcctgttcgc tgaaacaccg
    19681 cacgcttacc tgcaaggcgt ggcgctggaa gtgatggcga actccgataa cgtgctgcgt
    19741 gcgggtctga cgcctaagta cattgatatt ccggaactgg ttgccaatgt gaaattcgaa
    19801 gccaaaccgg ctaaccagtt gttgacccag ccggtgaaac aaggtgcaga actggacttc
    19861 ccgattccag tggatgattt tgccttctcg ctgcatgacc ttagtgataa agaaaccacc
    19921 attagccagc agagtgccgc cattttgttc tgcgtcgaag gcgatgcaac gttgtggaaa
    19981 ggttctcagc agttacagct taaaccgggt gaatcagcgt ttattgccgc caacgaatca
    20041 ccggtgactg tcaaaggcca cggccgttta gcgcgtgttt acaacaagct gtaagagctt
    20101 actgaaaaaa ttaacatctc ttgctaagct tattaaaggc ttataacacc ttcaggcggc
    20161 cagtccgcct gatttcattt tatggataat cattatgaat aaatcgctgg tagcggtagg
    20221 cgtcattgtt gcgctaggcg tagtctggac aggcggcgca tggtatacag gcaagaagat
    20281 tgaaacccat ctcgaagaca tggtcgcgca ggcgaacgcg caactaaaac tgacagctcc
    20341 tgaatccaac ctggaagtga gttatcaaaa ctatcatcgc ggcgtattca gcagtcagtt
    20401 gcaactgttg gtgaaaccca ttgccgggaa agaaaatccg tggattaaaa gcggtcagag
    20461 cgtcatcttc aacgaatcgg ttgatcatgg tcccttcccg cttgcccagc ttaaaaaact
    20521 gaacctgatc ccgtcgatgg catcaattca aaccacgctg gttaataacg aagtaagcaa
    20581 accactgttt gatatggcaa aaggtgaaac gccttttgag attaactcgc gcattggtta
    20641 cagcggtgat tccagttccg atatttcgct caagccactg aattacgagc aaaaggatga
    20701 aaaagtcgcc tttagcggcg gcgagttcca gttaaatgct gacagagacg gcaaagccat
    20761 ctccctttcc ggggaggcgc aaagtggtcg gatagacgca gttaacgaat acaaccagaa
    20821 agtgcagttg acctttaata atctgaaaac cgacggttcc agcacgctgg caagttttgg
    20881 tgagcgcgta ggaaaccaaa aactgtcact ggaaaaaatg accatttcag tggaaggcaa
    20941 agaactggca ctgctggaag gcatggagat cagcggtaaa tcggatctgg tcaatgacgg
    21001 taaaacgatc aatagccaac tggattactc gctaaacagc ctgaaggtac agaatcagga
    21061 tctgggcagc ggcaagctga ctttaaaagt cggccagatt gatggtgaag cctggcatca
    21121 gttcagccag caatataacg cgcaaactca ggcgctgctg gcacagccag aaattgccaa
    21181 caatcccgaa ctttatcagg agaaagtgac ggaagccttc tttagcgccc tgccgctgat
    21241 gttgaaaggc gatccggtga ttactgtcgc gccgctaagc tggaaaaaca gtcagggtga
    21301 aagtgcgctg aatctgtcgc tgttcctgaa agatccggca acgactaaag aagcgccgca
    21361 aacgctggcg caggaagtag atcgttcggt taaatctctg gatgcgaaac tgaccattcc
    21421 ggtggatatg gcaactgagt ttatgactca ggtagcgaag ctggaaggtt atcaggaaga
    21481 tcaagcgaaa aaactggcga aacagcaagt tgaaggtgca tcagcaatgg ggcagatgtt
    21541 ccgtctgacc accttgcagg acaataccat caccaccagc ctgcaatata ctaacggtca
    21601 gataacgtta aatgggcaga aaatgtcgtt agaagatttt gttggcatgt ttgcgatgcc
    21661 agctcttaac gttccggctg taccggcaat tccgcagcag taattcacgg ccaaaacgcc
    21721 ggagaatttt cacttcggcg ttttcatctg aaaacaaacc agagattgcc tgatgcgcta
    21781 cgcttatcag gcctaccttt tcctgcaata ctttgaattt atgagttttt gtaggctgga
    21841 taaggcgttt acgccgcatc cggcataaaa aacgcgcact ttgtcaacaa tctgaaacgc
    21901 cggaggtttt tctctccggc gttatttctt acttcagcat aaagtcatac tgaatactca
    21961 ttttcacatc ccagtaattc aggcgatcgc ccagcgtact gccatcgcgc ttttcccgcc
    22021 ctttatcggc agcaagaaag aaattcatcc cttgtaacgg cccttcgctg acccgatagt
    22081 tcataccacc taagataccc gtgccttcaa tccgcacatc tcgtttactg gattcatagc
    22141 cgcgagtcac aatcggtgca aatgtcagtg taaattgcgg cgttaaacga tagttgacgc
    22201 ccaattgcca ggactgcatt ccttcacggt tacgatcaag acttaatgaa aagggatagc
    22261 caatgtcggt atccaccacg gcaccattca ttgcatggcg caaataacca ccactgccaa
    22321 atacgcttcc ccacggcgca ttccaggtca attgtccact aaccaacgcc gtttcattgg
    22381 gctggctggt attacgactt aaaccttcga gctgcgcgta aaagccgagc aactcccctt
    22441 tcaacgtata attcgggtcg agcggtatgc ttttctcgag aatactttgt atctgattgg
    22501 aaacatcttt ctgtaccgat gcccccagag aatagcgact ggtatcatca tgccaactgg
    22561 cggctaaaaa agaacgtgcg cgatgtttat cgtattttcc cgtctggctg ttatacaccc
    22621 cttcatcacg aataccggtt gttgaatcag tccataaaaa tactcgtgaa cgccacattc
    22681 cccacagttc aatatttggt gttaaagccc aataacctaa tgctccttca gaaactgtcg
    22741 gtaatgtacg ttgcgaactg ccagccatta acggaatacg ataaggtaag ctaccataac
    22801 tataatccgt cccgaagcga cctaatttaa gcaggctatt ttcacagggt tttattttta
    22861 ctgcccccag ggcaaagcca aaacttttat caccatcaag ataccaccgg gtactcatat
    22921 cagcacgagc acctaattta taaacataat atgccccgcc ttcaacgcca ataatattgg
    22981 aataataacc actattgaaa tcgaacaatc ctctctgtac ccatgcgtaa atatctcttc
    23041 ccgcgccagc actgggctta tccgccctgc gcaattcgtt tttcagtctg atgcgtagtc
    23101 ccgcttcatc gtcagccagt tgcgccgcat aagcagaggt aaggccagag gcagccgtca
    23161 ggcaaataac ggccatggcc actatttttc tcatagttgc acctttgatg ttctgattat
    23221 ttttattgaa tattaattag tgatatcgct gattaattgc tgctgcactt ttttacgatt
    23281 atcaatttca accacgattt ctttgaattt tttatccgtg agcggataaa accagataat
    23341 aacgaatgcc agtagcataa atccgcaagg tactaaggca attgatgtgc ggatgcccat
    23401 aataacttcc ggcgtttgca cctgattggc gatatatccg cttaatccca aaataaaggc
    23461 aggaattgaa cctccgattg cctgaccgca tttacgggta aatgagaata gtgaataggt
    23521 gagcccttca attcgcacgc cggtcaggta ttcaccgtat tctacggtat cagcttccag
    23581 tgcccacatc acagtcatag taacgccctg accaattgaa gcgatggcca acgcaaccaa
    23641 cgccaccggc agtgaccaga cggaaaccca gaagaatagc agataaccgc aggttcccag
    23701 caaagcgcca atcaggaagg tattcttttt accgctcctc gcgaccatcc ccggcaccag
    23761 cggtgccgat gccacagtac caaccaagtt ttgcaccagt accagcacag tgaacagccc
    23821 ggtatcattt aacacatagc gctcgtagaa caacgacgag gcgctgaccg caaaggtcga
    23881 aatcagcaca cacagcgcac cgatgcacaa cataaacagc gggcgattcc gtttcagggt
    23941 ttgcagactg atcttcaatg acggctgcgc aacgatacgt accacattct cacgcgtcga
    24001 tttgaagcag atgaagtaaa gcaccattcc ggcaatcgcc agcacaatcg tccagaaatg
    24061 gtataccgac accatctctt ccgggctgga gttcttaatg ctcggcccta tcagaaatgc
    24121 caggcagaca aaggtcaatg aagcggcaat cccacgagcc gcgcccagac gggcgcggga
    24181 ttgtggttgt tgggtcatcg cggtagcaag tgaaccataa ggaatattca ccaggctata
    24241 gcaaagcccg aggcccatgt aggtcaaata tgcatacact actttgctgc tatggctcca
    24301 gtcggtcggt acccagaata ccagcacgct gaagatcatt aacggcgcag taccgaagag
    24361 taaaaacggg cggaattttc cccagcgggt attcacactg tccaccactc gtccggcaaa
    24421 gacgtcggcg aaggcatcga atacccgcac caataacagc atggtgcccg ccgcagcggc
    24481 accgacgcaa gcgacgtcgg tgtagtaact caacaggaag agcgccccca ttgcgaaggc
    24541 gaagttattg gcgacgtcac cgaggctgta gccgacgatg gtgcgccagg agagttgttg
    24601 attcattgtt tgcctccctg ctgcggtttt tcaccgaagt tcatgccagt ccagcgtttt
    24661 tgcagcagaa aagccgccga cttcggtttg cggtcgcggg tgaagatccc cttcttgtta
    24721 ccgccaacgc gcaatatgcc ttgcgaggtc gcaaaatcgg cgaaattcca tacctgttca
    24781 ccgacgccgg cgctgacgcg atcaaagacg cggtgataca tatccagcca tgcacactga
    24841 tactcttcac tccacatgtc ggtgtacatt gagtgcagcc cggctaacgt atccacgccg
    24901 tattcggtga tgataatcgg ctgatgcagt ttctcctgcc aggctagaag ttctttttcc
    24961 agtaccttct ctgccgtttc caaatcgccg ctttggacat accatccgta ataacggttc
    25021 aggcacagca catcaaagag atcgctgatg gtatcggtgt gagcgtcgca gaacattaca
    25081 ttgacgcagg tgatcggacg cgtcgggtcg agtttacgcg ttgcttccgc cagtggcgcg
    25141 aaatattctc gtgcaccttg cggacgggta tccggttcgt tggcaatact ccacatcacc
    25201 acgcttgggt ggtttttgtc acgcgctatc agctctttaa tcgcctgtaa atgcgcctgc
    25261 tgagtttccc cgttgacagc ctcttcgctg tacagttctt tcggcttgtt gcccgcttcg
    25321 aaaccaatgc ctaaagagag gttaaagccg acagctgcag tttcatcaat caccacgatg
    25381 ccatgttcat ctgcccactc gagcatctct tcagcgtaag ggtaatgcga ggttcggtag
    25441 gagttggccc caatccagtc cattaatgcg tgatcgtgca ccatcagcac gttatcgaat
    25501 cctttgcctc gcaaatccgc atcttcatga cgaccaaagc cagtaaagta gaacggtttg
    25561 tggttgttcg tccttcactg ccactgaccg gatgccgacg cgcagcgggt agatatcaca
    25621 ctctgtctgg cttttggctg tgacgcacag ttcatagaga taaccttcac ccggttgcca
    25681 gaggtgggga ttcaccactt gcaaagtccc gctggtacct tgtccagttg caaccacctg
    25741 ctgatccgca tcacgcagtt caacgctgac atcaccattt gctaccacct gccagtcaac
    25801 agacgcgtgg ttacagtctt gcgcaacatg cgtcaccacg gtgatatcgt ccacccaggt
    25861 gttcggcgtg gtgtagacca ttacgctgcg atggatcccg gcatagttaa agaaatcatg
    25921 gaagtaagac tgctttttct tgccgttttc atcggtaatc accattcccg gcgggatagt
    25981 ctgccagttc agttcgttgt tcacacaaac ggtgatacgt acacttttcc cggcaataac
    26041 atacggcgtg acatcggctt caaatggcgt atagccgccc tgatgctcca tcacttcctg
    26101 attattgacc cacactttgc cgtaatgagt gaccgcatcg aaacgcagca cgatacgctg
    26161 gcccgcccaa cctttcggta taaagacttc gcgctgatac cagacgttgc ccacataatt
    26221 acgaatatct gcatcggcga actgatcgtt aaaactgcct ggcacagcaa ttgcccggct
    26281 ttcttgtaac gcgctttccc accaacgctg atcaattcca cagttttcgc gatccagact
    26341 gaatgcccac aggccgtcga gttttttgat ttcacgggtt ggggtttcta caggacgtaa
    26401 cataagggac tcctcattca aataaaaata ctggtcaacc tttaatctga ttagttaacc
    26461 aattctgtgt tgcacacagt tatctgtgag aaacccatgg aagagaaatg gaaataaaaa
    26521 gaactgacac cgcgatcaaa aaaccaaaat ctgcaatacg cacagcgtga tatagatcgc
    26581 attaatcttt aaaacagcgt tgacagaagc cataaaataa gcaatcgaac gctcgttcga
    26641 gcatcttgag aacgataaac tctcatgaca tatggctacg gtgaatattt tgggggggaa
    26701 ttctccgatc ccggctagat tgtattttgt acagggaaat taaagtatga aacgcgggca
    26761 cagttatcac cagcccgcga aatgagagga tcaggatgcg gttaagatac cgccaatcat
    26821 cgcgcgtaat ccctgagcaa acgacgcttc acgcgccaga tcttccgctt cgatatcggc
    26881 cagtacaccg taggtcagat ccagcagtaa acgtgaagtc attgacgtgt tgatgtctgg
    26941 gcttatctcg cctttttgct gggcatcacg catccgctgg gcaacaaatt cagtgatcgt
    27001 catatgctta tttttaagca tggcggcgac ctgtgggtta cgcatccctt ccgccataat
    27061 ttcgaccacc agcgcccgtt gcccaaaggc ttcatgggtg agagagacaa tagactcgac
    27121 catatagtca acaaaatgaa tcccttcaat cggttcccgg aaacgggcca gcgccctctc
    27181 ctggtcctgt aagataatcg cctgaatcaa ggcttctttg gagatgaaat ggtgataaag
    27241 cgtcccggga ctaatggcgc aggatttaca gatggctttc atcgaggcac tgtgaaatcc
    27301 attttctgaa aaaatctctc tggcagcatt gaggatccgg gtccgtgtcg gttgtgcttc
    27361 agtctgcatg ttatccatca ttaaaccatt atagttagcg tgaataacta gtttaccgcg
    27421 ttgtcaggtg agcgcctatc attgctgttg aatagtgcga aggcacactc tattcgtatg
    27481 cttttcatcc agctattggt taaccaatag ccgtgatttg gctgccagtt gtttagcaga
    27541 gacgttgatg aattgcacac gctatcccgg taaacaacac atacggctgt ttaccggttc
    27601 gttagtgtat taattgagct cctgtacccc accaccggag acggtgagaa tttgtccgct
    27661 tacccagctg gctgcaggcg agcaaaggaa cagcgccgcg ttagcaatat cttgcggttg
    27721 acccagacgt ctgatcggcg tgtgctgtaa cattttttgt tcaatttctg gtgtaataac
    27781 ggatttcagg gcatcggtta atattgcccc cggcgcaatg ccattcaccc gaatattttt
    27841 ttcacccagg tcaaatgcca tatttctgac cagatgactg gccgcagctt tagatgatgc
    27901 ataggaagtc atgtttatat ttttattttc tgccgccata gaagtgatgg tcagaataac
    27961 gccaccgcca tttttttcca tttctggcgc aacaagttgt gacagatgga aaaaagaaaa
    28021 cacattcagt tcataagcac ggcgaaaatc cgccattggc atatcaaacg gtttaggtcc
    28081 accgccaccg gcgttgttaa ccagaatatc aactttaccc agcttactga tagcaaagtc
    28141 tgccagtgca gagagttcct gttcggaagt aatatcacaa cggcaggcaa atgcctgccc
    28201 ccccagttgt tgaatttcgt ctacaacatg gttagctgcg tcggcgttaa tatcactgac
    28261 caccacagat gcgccagctg tcgcgaatgt aatggcgatt tctttaccaa tacctgcacc
    28321 cgcacctgtg atgatggcgc attttccgtc gagtctcagg ttgtcagaat taaacacata
    28381 aacctcctgt gaacttcagt aaaaataaaa ggcaccgcct
//
LOCUS       DADLFA010000032        28224 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000032 DADLFA010000000
VERSION     DADLFA010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28224
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.032"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..856)
                     /locus_tag="JQH65_001202"
     CDS             complement(<1..856)
                     /locus_tag="JQH65_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HAY6138682.1"
                     /translation="MVKKPQQGTIFAAAQRSDSYFVKCINMLSLYEKIKIRLIILFLL
                     AALSFIGLFFIINYQLVSERAVKRADSRFELIQKNVGYFFKDIERSALTLKDSLYLLK
                     NTEEIQRAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKIVVYHQEQVNGPLVDES
                     GRVIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRPGKKCISFTLRINGKDHDLLAVD
                     KIHVDLNWRYLNEYLDQISANDEVLFLKQGHEIIAKNQLAREKLIIYNSEGNYNIIDS
                     VDTEYIAKT"
     gene            complement(956..1867)
                     /gene="gsiD"
                     /locus_tag="JQH65_001203"
     CDS             complement(956..1867)
                     /gene="gsiD"
                     /locus_tag="JQH65_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001236035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HAY6138683.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1870..2790)
                     /gene="gsiC"
                     /locus_tag="JQH65_001204"
     CDS             complement(1870..2790)
                     /gene="gsiC"
                     /locus_tag="JQH65_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HAY6138684.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILSSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2808..4346)
                     /gene="gsiB"
                     /locus_tag="JQH65_001205"
     CDS             complement(2808..4346)
                     /gene="gsiB"
                     /locus_tag="JQH65_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HAY6138685.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILVH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(4366..6237)
                     /gene="gsiA"
                     /locus_tag="JQH65_001206"
     CDS             complement(4366..6237)
                     /gene="gsiA"
                     /locus_tag="JQH65_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HAY6138686.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLCVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(6224..7189)
                     /gene="iaaA"
                     /locus_tag="JQH65_001207"
     CDS             complement(6224..7189)
                     /gene="iaaA"
                     /locus_tag="JQH65_001207"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAY6138687.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPAIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIGHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            7393..8628
                     /gene="moeA"
                     /locus_tag="JQH65_001208"
     CDS             7393..8628
                     /gene="moeA"
                     /locus_tag="JQH65_001208"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HAY6138688.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            8628..9377
                     /gene="moeB"
                     /locus_tag="JQH65_001209"
     CDS             8628..9377
                     /gene="moeB"
                     /locus_tag="JQH65_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HAY6138689.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLIVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHNDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEYDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(9453..10115)
                     /gene="fsa"
                     /locus_tag="JQH65_001210"
     CDS             complement(9453..10115)
                     /gene="fsa"
                     /locus_tag="JQH65_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAY6138690.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKNPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWLGAFGRT
                     SI"
     gene            10246..11145
                     /locus_tag="JQH65_001211"
     CDS             10246..11145
                     /locus_tag="JQH65_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HAY6138691.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEIATALLQACHEAGIHTAVETC
                     LHVPWKYITPSLPYIDLFLADLKHIADAPFKQWTDGNAAKVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APDLLDFAQQYACQKGLTATLRG"
     gene            11151..13583
                     /locus_tag="JQH65_001212"
     CDS             11151..13583
                     /locus_tag="JQH65_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HAY6138692.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQTLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGNVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13729..14544
                     /gene="ybiV"
                     /locus_tag="JQH65_001213"
     CDS             13729..14544
                     /gene="ybiV"
                     /locus_tag="JQH65_001213"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HAY6138693.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIAFDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14696..15961
                     /locus_tag="JQH65_001214"
     CDS             14696..15961
                     /locus_tag="JQH65_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HAY6138694.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSTSRPEIYPIYWSQTQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     HEDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(16202..17794)
                     /locus_tag="JQH65_001215"
     CDS             complement(16202..17794)
                     /locus_tag="JQH65_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HAY6138695.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLMKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            18013..18933
                     /gene="ldtB"
                     /locus_tag="JQH65_001216"
     CDS             18013..18933
                     /gene="ldtB"
                     /locus_tag="JQH65_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAY6138696.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(18992..20110)
                     /locus_tag="JQH65_001217"
     CDS             complement(18992..20110)
                     /locus_tag="JQH65_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HAY6138697.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKVLQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLASLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(20107..20574)
                     /gene="mntR"
                     /locus_tag="JQH65_001218"
     CDS             complement(20107..20574)
                     /gene="mntR"
                     /locus_tag="JQH65_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HAY6138698.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGTK"
     gene            20760..20888
                     /gene="mntS"
                     /locus_tag="JQH65_001219"
     CDS             20760..20888
                     /gene="mntS"
                     /locus_tag="JQH65_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HAY6138699.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLILLSMLCDMVNNKPQQDKPSDK"
     gene            21160..22743
                     /locus_tag="JQH65_001220"
     CDS             21160..22743
                     /locus_tag="JQH65_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HAY6138700.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(22792..23307)
                     /gene="ompX"
                     /locus_tag="JQH65_001221"
     CDS             complement(22792..23307)
                     /gene="ompX"
                     /locus_tag="JQH65_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HAY6138701.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(23360..23425)
                     /locus_tag="JQH65_001222"
     CDS             complement(23360..23425)
                     /locus_tag="JQH65_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6138702.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            23660..24547
                     /gene="rhtA"
                     /locus_tag="JQH65_001223"
     CDS             23660..24547
                     /gene="rhtA"
                     /locus_tag="JQH65_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HAY6138703.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            24846..25349
                     /gene="dps"
                     /locus_tag="JQH65_001224"
                     /gene_synonym="pexB"
     CDS             24846..25349
                     /gene="dps"
                     /locus_tag="JQH65_001224"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HAY6138704.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            25753..26499
                     /gene="glnH"
                     /locus_tag="JQH65_001225"
     CDS             25753..26499
                     /gene="glnH"
                     /locus_tag="JQH65_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HAY6138705.1"
                     /translation="MKSVLKVSLAALTLVFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            26638..27297
                     /gene="glnP"
                     /locus_tag="JQH65_001226"
     CDS             26638..27297
                     /gene="glnP"
                     /locus_tag="JQH65_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HAY6138706.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            27294..28016
                     /gene="glnQ"
                     /locus_tag="JQH65_001227"
     CDS             27294..28016
                     /gene="glnQ"
                     /locus_tag="JQH65_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017491583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HAY6138707.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
BASE COUNT         6977 a         7540 c         6974 g         6733 t
ORIGIN      
        1 atgtctttgc aatatactcc gtatcgacag aatcaataat attataatta ccttcgctat
       61 tataaataat cagtttttca cgagcgagtt gattcttggc aatgatctca tgaccttgtt
      121 tcaaaaatag aacttcatca ttagcgctga tttgatcaag atactcgttc aaataccgcc
      181 agtttaaatc gacatgaatt ttatctaccg ctaacaaatc gtgatctttg ccgttgatac
      241 gtagcgtaaa agagatacat tttttacccg gacgatcaaa gcaattgtat gccggattcc
      301 agctgttgtt agagtcatct gaagccaccg accacggtcg tttcgatggg ttaaaatcgg
      361 caaaaataac ccgccctgac tcgtcgacaa gcggtccatt tacttgttcc tgatgataaa
      421 caacgatttt atcattcgcc ctccgtgaaa agagataata tttattatca tcaagtacca
      481 gtcccaccga gtctaaaaat ggcatcattt ccattttcag aatcacggcg cgttgaatct
      541 cctctgtatt ttttaataaa tacagtgagt cctttaatgt cagggccgaa cgttcaatat
      601 ctttaaagaa atagccaacg tttttctgaa taagttcaaa gcggctatcg gcacgtttta
      661 ccgcgcgttc cgataccagt tgatagttaa tgatgaaaaa aagaccaata aatgacagtg
      721 ctgccaataa aaataaaatt atcagcctta tctttatctt ttcgtataaa ctcagcatgt
      781 taatacattt aacaaaataa ctatctgaac gctgtgccgc tgcaaaaata gtcccttgct
      841 gcggcttttt aaccaaatta cccgattcta caaaatcagc agaaagttac aacacttgct
      901 tagggcaata atttcctgtg aggtgattac cctttcaagc aatattcaaa cgtaactatc
      961 ctttaatttt cggatccagc gcatcgcgta aaccatcgcc caacaaattg aacgccagta
     1021 cggtcagaaa aatagccagg gccggaaaaa cagcgacatg cggcgcgata accatatccg
     1081 ctcgtgcctc attgagcatt gctccccact ctggtgtcgg cggctgcgca ccgaggccga
     1141 gaaatgacag gctggcggca gagataatcg aggtgccaat gcgcatggtg aaaaacacca
     1201 cgatagacga gacggtccca ggcaagatat gacgcaataa aatggtcata tcgctggcac
     1261 caatactgcg tgctgactca ataaaggttt gctgtttcaa caccagcgtg ttgccgcgca
     1321 ccagtcgggc aaacgcgggg atggaaaaaa tggcgactgc gataatcacg ttagcaatgc
     1381 cgcttcccaa caccgcaaca acagcgatcg ccagtaaaat acccgggaag gcaaacagca
     1441 catcgcaaat gcgcatgatc agccgatccc accagccttc ataatatcca gccaacaagc
     1501 ccagcaacgt cccgatcgcc gcaccgataa acacggcaaa cacgcccgcc gccagcgaga
     1561 tttgcgcacc aaccaggaca cggctgaaaa tgtcgcgccc cagtgaatcg acgccaaacc
     1621 agtgctgcaa agaaggtccg ttattcagat tgtcataatc aaaataattt tcggcgtcat
     1681 agggagcgat ccagcgtgca aaaatggcca ccacaatcaa taaaataacg aataatgcgg
     1741 cggtcatcgc catatgctga cggcgaaatc gtcgccagaa ttcatgccac ggtgtacgca
     1801 cctggtcagg tttgaccagt ggcatggcgt ttaacaccgc ctgacgtcgc cagttaaata
     1861 gtcgcatcct tacttgtacc tgatagccgg gttaatggcg gcgtaaagca catccaccac
     1921 taagttgata agaataaatt ccagcgagaa aagcagaatt tccgcctgaa tcaccgggta
     1981 atcgcgcatt tctacggagt caacgagtaa gcgtccaagt ccgggccagt tgaaaacttt
     2041 ctccaccacg atagaaccac cgagcaaaaa gccaaactgt aagcccatca tggtcactac
     2101 cgggatcatc gcgttacgta gcccgtgttt gaggacaacc caggtttcgc tcaccccttt
     2161 cgccctcgcg gtacgcatat aatcttcgct taaaacatcg acaaacgacg cgcgggtaaa
     2221 gcgcgccatc acggctgcca ctgccgcgcc gagcgtcagg gaggataaaa tgtagtgctg
     2281 ccaactgtct gctcccacgg taggcagcca gcccagttca acggagaata cctgaattaa
     2341 aagcatcccc agcgcaaatg ccggaaacga gatccccgac accgcaatgg tcatactcaa
     2401 tcgatccggc caacgattac gccagacggc ggcgataatt cccgccgcca taccaaatat
     2461 caccgcccag accatactgg ttatggtcag ccacagcgtt ggcataaagc ggctggcaat
     2521 ctcatcggca accggacgac gcgacaccat cgacaggcca aaatccccct gcacagcatt
     2581 gctgatatag tgccagaact ggtgatacag cggctgatcc aaccccagct gctgacgcac
     2641 cagttctata acctgcgcat cagcttcggg cccggcaatc aatcgcgccg gatcgccggg
     2701 cagcatatgc acaaataaaa acaccagcac cgagacgata aacagcgtcg gaatcaaccc
     2761 cagtaagcgt ttgataacgt aattaagcat tccactccct gcgttgctta ttgcaaatcc
     2821 gcgtcttcaa agctgaagcc ggtgtctggc atgatccaaa aaccggtcag gtttttactg
     2881 tgtgccgaca ccagtttttc taccaccagc gggatccacg gcgactcttg ccagatgata
     2941 tcctgcgccg ccttatacaa gcgggtcttt tccgccggat cattagtttt cagtgcctga
     3001 gccaggaagt cgtccacctg tttattgctg taaaatgcgg tattaaacag cgtcggtggc
     3061 cagttttgcg aggcgaacag tggcgatagc gcccagtccg cttcgccggt tgaagccgac
     3121 cagccagtgt agaacatccg cacgccgctc tctttttgcc ctttaccttc aacttctgcc
     3181 gcccgctgtc cggcatccat cgcggtcacc tgggctttaa tcccgacctg cgctaactgc
     3241 tgctgggtaa attgcagcac tttctgcgcg gtgctgtggt tatgtgacga ccacagcgtg
     3301 gtactgaaac cgttgggata tcccgcctct ttcagtaatt cgcgcgcttt cactggatcg
     3361 taaggccacg gtttataact ttgcgcgtag gcgatacttg gcggtaccac accagtagct
     3421 ggcgttgcat agcccgcaaa ggcaactttc accagcgccg gacggttaat ggcgtaattc
     3481 agcgcctcac ggaccttcgg gttatcgaac ggcttttgcg tcacgttcat actgatataa
     3541 cgctgcataa ttgacggact ggccatcaac tcgatatttt tgtttttctc cagcagtgcg
     3601 gcctgctcgt agggaatggg gaaagcaaac tgcgcttcac cggtttgcag cattgccgcg
     3661 cgggtgttgt tatccgccac cggacgccag gttatgctgt ccagtttggg caatcctggc
     3721 tgccagtaac ccgcgaattt tttcaccttc acaaaatcgg tctgattcca ggtatccagt
     3781 tcatacggtc cggttcccac cggatgaaaa ccaatctcct tgccatattt ttccagcgct
     3841 gccggtgaaa tcatcgcggt cgccggatgg acaagaatat taataaacgc tgagaacggc
     3901 tgtttgaggg taatctttac cgttgtcgga tcgatcgcct ccgttttggc aatattctta
     3961 tacaggttat agcgtttaag atgattcgcc ggatcgctgg cccggtccag attcgctttc
     4021 accgccgcgg cgttgaaatc ggtgccatcc tggaatttaa ttccttcccg caatttcacg
     4081 gtgtaagtaa tgccgtcatc ggaaacggta taactctccg ccagcacgtt tttcagtttc
     4141 atctctttat ccagaccgaa cagcccctgg taaaacgatt tcgctacggc ctgagataac
     4201 gtgtcattcg cgtcatacgg atcgagcgtg gtgaaattcg atcctaccgc caccaccaca
     4261 tctttggcag cgaatgcaca agatgccatc aacgctgtcg caatgcccag cgccactaac
     4321 ccactacggt gtacagctct tgccatttta ttctccgcct gaatgttatc tacgcatgaa
     4381 tgcgtattct gattgtggtt gtgcgacgta atgccccggc ccgacgcatt gcaacgagac
     4441 ggctgccacc tcttcgccac gcagatgaat attgctggga agatcgtccg acagcagtac
     4501 acgctgcggt cgttgtcggg acggttcagc gaccggaact gccgccagta atttacgcgt
     4561 ataaggatgc tgcgggtttt cgaagaccgc gcgccgtgga ccaatttcaa caatttgccc
     4621 gagatacatc accgccacac gatgactaat ccgctctacc acggccatat cgtgggagat
     4681 aaacagatac gcaatgccga aatcacgctg gagatcgagc aacaagttga taatctgccc
     4741 gcgaatagaa acatccagcg ccgaaacggc ttcgtcggca atgatcactt ttggattcaa
     4801 tgccaacgcg cgagcaatgc agatgcgctg gcgctgaccg ccggaaaact catgcgggta
     4861 acgccaggca tgttcaggta acaggcccac gcgctccagc aaccacgcaa cgcgtgcagc
     4921 cgcgtcttta cctggcaata aaccgtgtac acacagcggt tcgataatcg aatcaccgat
     4981 ggtctgacgt gggtccagcg aagcgtaagg gtcctgaaaa ataaactgaa tatcccggcg
     5041 caatgcctga agtttgccag gtgacaaggt atcgattcgc tgaccgttaa agataatttc
     5101 gccgccctgc gattcgacca ggcgcagcaa cgcccgcccg gtagtggatt taccgctgcc
     5161 agactcgccc accagcgata gcgtttcgcc aggccagaga tcaaaactga ctttctcaac
     5221 ggcatgcact tcccgcgtta cgcgattcaa caaaccgctg cgcaaaggga aacgggtgac
     5281 aagattacgc actcgtaaaa caggttcgcc atccaccacc gttttctgct cgatgggggg
     5341 ttcctgtttc gctggatgtt caagcgatat caatgggaaa cgtcggggat aatctaaccc
     5401 tttcatcgca ccaagttgcg gaacagcagc taacagcgca cgggtgtaag gatgttgcgg
     5461 tgcatgaaaa atctgttcga cggtacccgt ttccaccgcc tcgccctgat acatcaccag
     5521 tacccgatcg gctatctctg ccaccacgcc catatcgtgc gtgataaaga taacgcccat
     5581 cgacatctct ttttgcaata ctttgattaa ttgcaggatc tgcgcctgaa tagtgacatc
     5641 cagcgcggtg gttggctcat cggcaatcag caccgccggg cggcatgaca gcgccatcgc
     5701 aatcatcact cgctggcgca tcccgccaga gagttgatgc ggataacgtg aaagaatggt
     5761 ttgtgcctca ggaatgcgta cctgatccag catccgcttc gcctcgacca tcgcttcttc
     5821 acgactggcg ttctgatgca gacgaattga ttcggcaatc tgttcaccca cagtaaatac
     5881 cgggttcagc gatgtcatcg gctcctgaaa tatcatcgcc atatccgcac cgcgaacatg
     5941 gcgcatttgt gcagcgctct gctcgctaag ttcaatcact tcgcgactgc gccgctgcaa
     6001 cagcatttta tcgcactgta ctaaaccgcc cgcctgttcc aacaggcgca tcaatgccaa
     6061 cgcagtcact gacttaccgg agccggattc gccaacaatt gccagcgtct caccgcgttg
     6121 cagactaaaa gagagattgc ggaccgcagc tattttctgc tggtcctgca taaaggcaat
     6181 attcagattt tcaaccgcca gcacattacc ggcatcaagt tcatcactgt gtggcaacgg
     6241 tgtccccttt ttcacggtag ataccggtgg ttggcgtatc gcctgcgtag ccccaggcgc
     6301 gatacattcc ttcggtgtta aacggtagcg cgacattccc ttcatggccg atagcgatta
     6361 agccaccgct accgccaagc gcagggagtt tttccattac tacccgctcg caggcttccg
     6421 cgagacttaa tccgccgtaa tccattaacg cggcgatgtc atatgccgcc agcgcgcgga
     6481 tgaagacttc gcccgtgccg gtacaagaaa ccgccacact ggcgttattg gcgtagcatc
     6541 cggcacccac taagggacta tcgccaactc gtccgggtaa tttattggtc attccgcctg
     6601 tggacgtggc tgccgccaaa ttgccgtcta aatccaacgc cacggccccc acggtgccca
     6661 ttttctgttt ttcatccagt ggcgcaccgc tatggtcgag gacggttgcc ccttccttgc
     6721 gcgctgccag tagttgttca taacgcaaag gcgtggagaa aatcgccggc gagacgcgct
     6781 ccatgccacg agcaaacgca aaattttctg ccccttcgcc aatcatcatc acatgcgggc
     6841 tttgctccat caccagccgg gcggcaagaa ccggattacg cagatgacta acgcccgcca
     6901 ccgcaccggc tttcagggtg ttaccatcca tcacacaggc gtccagttca tgggtttcat
     6961 cacgcgtaaa gacagcgcca attccggcgt taaacagtgg acactcttcc agcagacgca
     7021 ccgcttccgt caccacatcc agcgcacttt cgcccgcttc cagcattttc tgcccggttt
     7081 caacaatggc agacaacgcc tcaatgtagc gtaattcctg ttgcagactc atctgcgcgc
     7141 ggctaattgc acctgcgcca ccatgaattg caatgactgc tttgcccata gtattcgtcc
     7201 attaagggtg gctcgccact tttgctataa atatcagaat ttttatgaat tactcattca
     7261 atttttgaat atagaaagat ggatatggca tgtaaaggca ggagccaacg ctacgcccga
     7321 ggtggcatac agtctgtggc gtttttctga cataataggc aaattcgatt ttgcctccgc
     7381 aggagtgttt tcatggaatt taccaccgga ttgatgtcgc tcgacaccgc gcttaatgag
     7441 atgctttctc gcgtcacccc actgaccgcc caggaaacgc tgccactggt acagtgtttt
     7501 ggtcgtattc tggcgagcga tgtcgtttcg ccgcttgatg ttccggggtt tgataactcc
     7561 gcaatggacg gctacgcggt gcgtttagcc gatattgcct ccgggcaacc gctgcccgtt
     7621 gccggtaaat cctttgccgg tcagccgtat catggtgaat ggcctgcggg cacctgcatt
     7681 cgtattatga ccggtgcgcc ggtgccggaa ggctgcgaag cggtggtgat gcaggagcag
     7741 actgaacaaa cggacaatgg cgtgcgtttt actgctgaag tgcgtagcgg gcaaaatatt
     7801 cgccgtcgcg gtgaagatat ctctgcaggt gcggttgttt tcccggcggg aactcgcctg
     7861 actaccgcag agctgccagt aattgcttca ctggggattg ccgaagttcc ggtgattcgt
     7921 aaagtgcgtg tagcgctttt ttctaccggt gatgaactcc agttgcccgg tcagccgctg
     7981 ggtgacggcc aaatctacga caccaaccgt ctcgccgtac acctgatgtt agaacagctg
     8041 ggttgcgagg taatcaactt agggattatc cgcgacgatc cccatgccct gcgcgccgca
     8101 tttattgaag ccgacagcca ggcggatgta gtgatcagtt ccggcggtgt ttcagtgggt
     8161 gaagcggatt acaccaaaac gattcttgaa gagctggggg agatcgcctt ctggaagctg
     8221 gcgattaaac caggtaaacc gttcgcgttc ggtaaactca gcaatagctg gttctgcggc
     8281 ctgccgggca acccggtttc agcgacgctg accttctatc aactggtaca gcctttgctg
     8341 gcaaaactaa gcggcaacac cgccagcggc ctgcccgcgc gccagcgcgt acgcacagcg
     8401 tcccgcctga aaaaaacgcc aggacgtctt gatttccagc gcggcgtgct gcaacgcaac
     8461 gccgacggtg aacttgaagt gacgaccacc ggacatcagg gttcacatat atttagctcc
     8521 tttagtctcg gcaactgctt tatcgtgctg gaacgcgatc gcggcaatgt ggacgtgggc
     8581 gaatgggtgg aagtagaacc gtttaacgcg ttgttcggag gcctgtaatg gcggaactca
     8641 gcgatcagga gatgctgcgc tacaaccggc aaatcattct gcgcggtttt gattttgacg
     8701 gtcaggaggc gctaaaagat tctcgtgtgc tgatcgtcgg cctcggtgga ctcggctgcg
     8761 cagcttcgca gtatctggca agcgccggtg tcggcaacct gacgctgctc gacttcgaca
     8821 cggtttcgct ctcgaatctg caacgccaga cactgcacaa tgatgccacg gtcgggcaac
     8881 cgaaggtgga atccgcccgt gacgccctga cgcggatcaa cccacatatc gcgattacgc
     8941 cagtcaatgc actgctagat gacgcagaac ttgcagcatt gattgctgaa tacgatctgg
     9001 tgctcgactg tacggataac gttgcggtac gtaatcaact gaacgcaggc tgttttgccg
     9061 cgaaggtgcc gctggtttcc ggcgcggcaa ttcgtatgga agggcaaatc acggtcttca
     9121 cttatcagga cggcgaaccg tgctatcgct gccttagccg tttgtttggc gagaacgcgt
     9181 taacctgcgt agaagcaggc gtgatggcac cgttgatcgg cgtaattggt tcgttgcagg
     9241 caatggaagc gatcaaactg ctggcaggtt atggcaaacc ggccagcggg aaaatcgtca
     9301 tgtacgatgc gatgacctgt cagtttcgcg aaatgaaact gatgcgtaat ccggggtgtg
     9361 aggtgtgcgg gcagtaattg ccacctccag gccagataag acgcggcact ccgccacgtc
     9421 atcagcaatt tatgaggatg cagaacgtcc ggttaaatcg acgttctgcc aaacgctccc
     9481 agccagtcct gttcaaactt cgccacagcg gcatcaaccg ccggatagct aatcatctgt
     9541 tgtgccacat ccagtggcag cgtaattgat tcacatcctg ccagtaagca gtccagcgcc
     9601 tgacgcgggt ttttgaaact cgctgccagc actttcgcct gcggcgcatg cattttcaat
     9661 aactggtgta agtcggtcac agtctgaatg ccgctaccgc cctgagcatc aatacgatta
     9721 acgtaaggcg caacatattc cgcacctgcc agcgccgaca gcagcccttg tgctgcgcca
     9781 tataccgcgg ttcccagcgt tggaatccct tccgctttta acatcttaat agctgccagc
     9841 ccctcggcgg tcaccggaac tttcaccacg atatccgcaa taatagaacg caatttacgt
     9901 gcgtcattca ccatcccttc agcagtggta gccattacct gggcaaacag acgcccctga
     9961 ccgcccatcg cttcatgaag ttgcggaagc acaacatcca gcggtttttt acccgcggcg
    10021 ataatgcttg ggttagtggt cacacccgcc agcggaaaaa tacgtgacag cgccttcact
    10081 gcaacaacgt ctgaagtatc cagatacagt tccataacca tcctcaaaat gtactttttg
    10141 tctgaatgta gactatcacg ggaaaactct ctgaacagtt gacgcacatc aatccaactt
    10201 tcattcgaaa gtattttaat gtttgaacga aacagagagg caactatgat tttcaatatt
    10261 cagcgctact cgacccatga tggccccggt atccgcacgg tcgtatttct taaaggctgt
    10321 tcgctgggct gccgctggtg tcagaacccg gaaagccgcg cccgcacgca ggatctgctg
    10381 tatgacgcac gactgtgtct ggaaggctgc gagctgtgcg ctaaggcagc gccggaagtg
    10441 attgagcgcg cattgaatgg tttgcttatt catcgggaaa agttaacccc ggagcatctg
    10501 acggcgctaa ccgactgctg cccgacacag gcgttaaccg tgtgtgggga agtgaaaagc
    10561 gttgaggaga tcatggcgac cgttctgcgc gataaaccgt tttacgatcg cagcggcggc
    10621 ggcttaacgc tttcgggtgg tgaacccttt atgcagccgg aaatagcgac ggcgctactg
    10681 caagcctgcc acgaggcagg cattcatact gcggtagaaa cctgtctgca tgtgccgtgg
    10741 aaatatatca ccccttctct gccctatatc gatctgtttc ttgccgattt aaaacacatt
    10801 gccgacgcgc cgtttaaaca gtggaccgac ggtaacgccg ccaaggtgct ggataacctg
    10861 aaaaaactcg ccgcagcggg caaaaaaatc attatccgcg tgccgctgat tcagggcttt
    10921 aatgccgacg aaacctctgt aaaagccatt accgattttg ccgccgacga gctgcacgtt
    10981 ggcgaaattc attttctgcc ctaccacacg ctgggcatca acaaatatca cttacttaat
    11041 ctgccctatg acgccccgga aaaaccgctt gatgcgccag acctgctcga ctttgcccag
    11101 cagtatgcct gccagaaagg gttaaccgcg accttacgag gataacaacc atgaccacac
    11161 tgaaactgga cacgctcagc gaccgcatta aagcgcacaa aaatgcgctg gtgcatattg
    11221 tgaaaccgcc ggtctgtacc gagcgcgcgc agcactatac cgagatgtat caacaacatc
    11281 tcgataagcc gatcccggta cgtcgcgcgc tggctctggc gcatcacctg gcgaatcgca
    11341 ccatctggat caagcacgat gagttgatca tcggcaacca ggcaagcgag gttcgcgctg
    11401 cgccgatctt cccggaatat accgtctcgt ggatcgaaaa agagattgat gatctggcag
    11461 atcgtcccgg tgctggtttt gcggtgagcg aagagaacaa acgcgttctg catgaagtgt
    11521 gcccgtggtg gcgcggtcag acggtacagg atcgctgcta cggcatgttt accgatgagc
    11581 aaaaaggtct gctggcgacc ggaatcatta aagcggaagg caatatgacc tccggcgatg
    11641 cgcacctggc ggtgaatttc ccgctgctgc tggaaaaagg tcttgatggt ctgcgcgaga
    11701 aagtagcgga acgtcgctcg cgcatcaatc tgacggtact ggaagatttg cacggcgaac
    11761 agttcctgaa agcgattgat atcgtgctgg tggcagtcag cgaacacatt gaacgttttg
    11821 cagccctggc gcgtgaaatg gccgcgaccg aaacccgcga aagccgtcgc gatgaactgc
    11881 tggcgatagc agaaaactgc gatcttatcg cccaccagcc gccgcagact ttctggcagg
    11941 cgctgcaact gtgttacttc atccagttga tattgcagat cgaatctaac ggtcactcgg
    12001 tatcgtttgg tcgtatggac cagtatctct acccgtacta tcgccgagac gttgaactca
    12061 accagacact ggatcgcgaa cacgccatcg aattgctgca tagctgctgg ctgaaactgc
    12121 tggaagtgaa caagatccgc tccggctcac actcaaaagc ctctgcggga agtcctctgt
    12181 atcagaacgt caccattggc ggacaaactc tggttgatgg tcaaccaatg gacgcggtga
    12241 atccactctc ttacgcgatc ctcgaatcct gcggtcgcct gcgttccact cagcctaacc
    12301 tcagcgtgcg ttaccatgca ggaatgagta atgatttcct cgacgcctgc gtacaggtga
    12361 tccgttgcgg cttcgggatg ccggcgttca acaacgacga aatcgtgatc ccggaattta
    12421 ttaaactcgg tattgaaccg caggacgctt acgactacgc agcgattggt tgtatcgaaa
    12481 ccgccgtcgg tggcaaatgg ggctatcgct gtaccggcat gagctttatc aacttcgccc
    12541 gcgtgatgct ggcggcgctg gaaggcgggc gtgatgccac aagcggcaac gtgttcctgc
    12601 cacaagaaaa agcgttgtcg gcaggcaact ttaacaattt cgatgaagta atggacgcgt
    12661 gggatacgca aatccgttat tacacgcgta aatcaatcga aatcgaatat gtcgtcgaca
    12721 ccatgctgga agagaacgtg cacgatattc tctgctcggc gctggtggat gactgtattg
    12781 agcgagcgaa aagtatcaag caaggcggcg cgaaatatga ctgggtttct ggcctgcagg
    12841 ttggcattgc caacctcggc aacagcctgg cggcagtgaa gaaactggta tttgaacagg
    12901 gcgcgattgg tcagcaacag ctggctgccg cactggcgga tgacttcgat ggcctgactc
    12961 atgagcaact gcgtcagcgg ctgattaacg gtgcgccgaa gtacggcaac gacgatgata
    13021 ctgtcgatac gctgctggct cgcgcttatc agacctatat cgacgaactg aaacagtacc
    13081 ataatccgcg ctacggtcgt ggtccggttg gcggcaacta ttacgcgggt acgtcatcta
    13141 tctccgctaa cgtaccgttt ggtgcgcaaa ctatggcaac gccggacggg cgtaaagccc
    13201 acaccccgct ggcagaaggc gctagtccgg cctctggtac tgaccatctc ggccctactg
    13261 cggtcattgg ctcggtgggt aaactgccta cggcagcgat tctcggcggc gtgttgctca
    13321 accagaaact gaatccggca acgctggaga acgaatctga caagcagaag ctgatgatcc
    13381 tgctgcgcac cttcttcgag gtgcataaag gctggcatat tcagtacaac atcgtttccc
    13441 gcgaaacgct gctggaggcg aaaaaacatc cggatcagta ccgcgatctg gtagtgcgtg
    13501 tcgcgggtta ttccgccttc ttcaccgcgc tctctccaga cgctcaggac gatatcatcg
    13561 ctcgtactga acatatgctg taatctcttc ggcccggctg cggcgtcgcc gggctaaatc
    13621 attcacatca tcaatttcat ccttactttc attcgaaata taatttgtgc tctgcgtcac
    13681 attgttatta cactgttacg gtttcatttt cgagccagga gcacacctat gagcgtaaaa
    13741 gttatcgtca cagacatgga cggtactttt cttaacgacg ccaaaacgta caaccaacca
    13801 cgttttatgg cgcaatatca ggaactgaaa aagcgcggca ttgagttcgt tgtcgccagc
    13861 ggtaatcagt attaccagct tatttcgttc tttcctgagc taaaggatga gatctctttt
    13921 gtcgcggaaa acggcgcact ggtttacgaa catggcaagc aactgttcca cggcgaactg
    13981 acccgacatg aatcgcggat tgttattggc gagttgctaa aagataagca actcaatttt
    14041 gtcgcctgcg gtctgcaaag tgcatatgtc agcgaaaatg cccccgaagc atttgtcgca
    14101 ctgatggcaa aacactacca tcgcctgaaa gctgtaaaag attatcagga gattgacgac
    14161 gtactgttca agttttcgct caacctgcca gatgaacaaa tcccgttagt gatcgacaaa
    14221 ctgcacatag cgttcgatgg cattatgaaa cccgtcacca gtggttttgg ctttatcgac
    14281 ctgattattc ccggtctaca taaagcaaac ggtatttcgc ggttactgaa acgctgggat
    14341 ctgtcaccgc aaaatgtggt agcgattggc gacagcggta acgatgcgga gatgctgaaa
    14401 atggcgcgtt attcctttgc gatgggcaat gctgcggaaa acattaaaca aatcgcccgt
    14461 tacgctaccg atgataataa tcatgaaggc gcgctgaatg tgattcaggc ggtgctggat
    14521 aacacatccc cttttaacag ctgacctctc taccggagcc gtgcgctccg gttttccctt
    14581 ctttttacat cctgtacatc acatcaaatc attaagcttg cattaactta tttataactt
    14641 atattgtcac atgcaaatca taatggtttc gaatgaaagt taatgagagg tggttatggc
    14701 ttctactttt accagcgaca cattgccagc cgatcacaaa gcagctatcc gtcagatgaa
    14761 gcacgcgctg cgggcgcagc ttggcgacgt ccagcagatc tttaatcagc taagcgatga
    14821 cattgccacg cgagtggctg aaatcaacgc actcaaagca cagggcgatg ccgtctggcc
    14881 ggtgctgtct tatgccgata tcaaagcagg tcatgttact gcagagcagc gcgaacagat
    14941 taaacgtcgc ggttgtgcgg tgataaaagg ccatttcccc cgcgaacaag cgctaggctg
    15001 ggatcagtcg atgctggact atctggaccg caaccgcttt gacgaggtct acaaaggccc
    15061 cggcgataat ttcttcggga cgctcagcac ttcacgtccc gagatttacc ccatctactg
    15121 gtcgcagacg caaatgcagg cccgccagag tgaagaaatg gcgaatgcgc agtcgtttct
    15181 caatcgtctg tggacatttg aaagtgatgg aaagcaatgg tttaacccgg atgtgagcgt
    15241 catctaccct gaccgtatcc gccgccgtcc gcccggaacg acctccaaag gtcttggagc
    15301 gcataccgac tccggggcgc tggaacgctg gctgcttcca gcgtatcagc gcgttttcgc
    15361 caacgtcttt aatggcaatc tggcgcaata tgatccctgg catgcggcac atcgtacgga
    15421 agttgaagag tacacggtgg acaacaccac caaatgttcc gtgtttcgga cattccaggg
    15481 ctggacagcg ctctctgata tgctgcctgg tcaggggttg ctgcacgttg tgcccattcc
    15541 tgaagccatg gcgtacgtac tgttacgtcc gctgcttgat gatgtgccgg aggatgaact
    15601 gtgcggcgta gcgcccggaa gagtgttgcc ggtatcagag caatggcatc cactgttaat
    15661 tgaggcgtta accagcattc caaaactcga ggccggagac tccgtctggt ggcactgcga
    15721 cgtcatccat tccgttgccc ccgttgaaaa tcaacagggt tggggcaacg tgatgtacat
    15781 tcctgcggca ccgatgtgcg agaaaaatct tgcctacgcg cacaaggtga aggccgcact
    15841 ggaaaaaggc gcatcgccgg gcgacttccc gcacgaggac tatgaaacaa actgggaagg
    15901 acgctttacg cttgccgacc tcaacattca cggtaagcga gcgttgggca tggatgtttg
    15961 agagttaatg gaagtgaatt gatgtgggat taagaaaatc gattcgacgt tactcagatt
    16021 gctgacaagt gcgctttctt aatgcctgat gcggcgttaa cgccttatct ggcctacaaa
    16081 gtcctgcaga ttcaatatat tacaggtact gcgtaggcct gataagcgta gcgcatcagg
    16141 ctattttgca tttgtcatca gccgccgtca ccccgacagt atcggggtga cggataatca
    16201 cttactcgat ccctttacta cgcaggtaat cttcgtaatt accgctaaag tcgatcacgc
    16261 gttccggggt gatttccaga atgcgggtcg ccagggagct tacgaactca cggtcgtgtg
    16321 aaacaaagat cagcgtgccc tgatacagtt ccagcgccat gttcagtgac tcgatggatt
    16381 ccatatccag gtggttggtc ggttcgtcca tgatcagaat gttcggcttc tgcatcatta
    16441 acttaccaaa cagcatccgc cctttttccc caccggaaag cactttagct ggctttttga
    16501 tgtcgtcctg gctgaacagc aaacgaccga gaatgctgcg taccgcctgc tcgtcatcgc
    16561 cttcctgctt ccactgactc atccattcga acacggtcag atcattttca aactcatatt
    16621 cgtggtcctg ggcgtagtaa ccaatgcgcg cgttttcaga ccattttacg gtgccgctgt
    16681 ccggttgcag atcgcccacc agcgttttca tcagcgttga tttaccgacg ccgttggtac
    16741 ccagtaccgc aagtttttca ccgacctcca gcagcaggtt gagattttta aacagtggac
    16801 cgttatcaaa ccctttggtc agaccttcca cttccagcgc gttacggaac agtttcttat
    16861 cctgttcaaa acggatgaac gggttctgac ggctggaggc tttcacctct tccagtttga
    16921 ttttatcaat ctggcgcgcg cgcgaagttg cctggcgaga tttcgaggcg ttggcgctaa
    16981 agcggctaac gaaagattgc aactcagcaa tctgcgcttt cttcttggcg ttatcggcca
    17041 gcagacgttc acgcgcctgg gtcgccgccg tcatgtactc atcgtagtta cccggataaa
    17101 cgcgcagctc gccgtaatcc agatccgcca tgtgggtaca gaccatgtta aggaagtgac
    17161 ggtcgtgcga gatgatgatc atggtgctgt cacgctcgtt cagcacctgt tccagccagc
    17221 gaatggtgtc gatgtcgagg ttgttggtcg gttcgtcgag caggagaata tccggatcag
    17281 caaacagcgc ctgcgccaga agcacacgca gcttccagcc aggagcaact tcactcatcg
    17341 ggccgtagtg ctgttccact ggaattccca cgccaagcaa caattcaccg gcgcgagctt
    17401 ccgcagagta accgtccatt tcgccgtatt taacttccag atcggccact ttatagccgt
    17461 cttcctcact catttccggc aaagcataga tgcggtcacg ctcctgcttc acttcccaca
    17521 actctttatg ccccatgatc accgtatcca gcacagtgaa ctcttcaaag gcaaactgat
    17581 cctgacgcag cttaccaatg cgctcgttgg gatcgaggga aacgttaccc aaagtcggct
    17641 ctaagtcgcc gccgaggatc ttcataaagg tggatttacc actaccgttc gcgccaatca
    17701 ggccgtaacg gttgccgccg ccaaatttga cggaaatgtt ttcaaacaac ggcttactgc
    17761 cgaactgcat ggtgacgtta ctggaaacta acacaggcgt atcctgaaaa gagatatgac
    17821 aaaccgcgca ttatgccata agtctgaaag aagatcctgc tcctcgctta aggtctacac
    17881 tcaagccaaa ggcaaaaaat gtgatttcgt acacatctga tttcactgtg agctggaatg
    17941 aacttataat gcgcttccaa tactctaata ttctcaaccc aatggcctgc caggcacaaa
    18001 atctcgctta acatgaatat gaaattgaaa acattattcg cagcggcctt cgctgttgtc
    18061 ggcttttgca gtaccgcctc tgcggtaact tatcctctgc caaccgacgg gagtcgcctg
    18121 gttggtcaga atcaggtgat caccattcct gaaggtaaca ctcagccgct ggagtatttt
    18181 gccgcggagt accagatggg gctttccaat atgatggaag cgaacccggg tgtggatacc
    18241 ttcctgccga aaggcggtac tgtactgaac attccgcagc agctgatcct gccggatacc
    18301 gttcatgaag gcatcgtcat taacagtgca gagatgcgtc tgtattacta tccgaaaggg
    18361 accaacaccg ttatcgtgct gccgatcggc attggtcagt taggcaaaga tacgcctatc
    18421 aactggacca ccaaagttga gcgtaagaaa gcaggcccga cctggacgcc gaccgcaaaa
    18481 atgcacgcag agtaccgcgc tgcgggcgaa ccgcttccgg ctgtcgttcc ggcaggtccg
    18541 gataacccga tgggtctgta tgcactctac atcggtcgcc tgtatgctat ccatggcacc
    18601 aacgccaact tcggtatcgg cctgcgtgta agtcatggtt gtgtgcgtct gcgtaacgaa
    18661 gacatcaaat tcctgttcga gaaagtaccg gtcggtaccc gcgtacagtt tattgatgag
    18721 ccggtaaaag cgaccaccga gccagacggc agccgttata ttgaagtcca taacccgctg
    18781 tctaccaccg aagcccagtt tgaaggtcag gaaattgtgc caattaccct gacgaagagc
    18841 gtgcagacag tgaccggtca gccagatgtt gaccaggttg ttcttgatga agcgattaaa
    18901 aaccgctccg ggatgccggt tcgtctgaat taatcttcaa aacttaaagc aaaaggcgga
    18961 ctcataatcc gcctttttta tttgccagac cctagttggc cgggagtata actaacaaaa
    19021 catatcccac caacgccgcc cacaacagca tcggtattga atagagatgg aaacgccacc
    19081 agatgcggcg atcgttcgcc atacgtagcg caatcaaact tgccagcgat ccgggtaata
    19141 acccaaagcc acctacgttt accgcccatg ccagtaataa agacggcggc acatagttca
    19201 gcaacaatat ggtactcggc acgttactga tcacctgcga taaaccgatt gccgttaacc
    19261 ataacccggg ttcagataga tgactcacgt tacccaacac gccttgcaac gctggaagct
    19321 gggtcagtaa atggacgtcg ataaacatcg ccataaacac cagcagcagc gtccagtcca
    19381 cactgagcac cacgcgacgt gccagcagcg caaagcctgc cgccacaatc accagtcccc
    19441 acagctcttg tttgaactcc agcgccgtta gaaagacgat atacagcccc agacaactcc
    19501 acaccagccg cggtttccac tccggtgttt gcacccccgt atggtattgc agcacctttc
    19561 cagggaaaca acaccagcac aggagcagga gcgtcagcat cattgcgcca gccagcggtg
    19621 ccatttgggc aataaatccg gcaaacgaaa gaccagaacg tccccagata agaatatttt
    19681 gcgggttgcc aattggcgtc agtagcgaac cagcattgac tgccagcgcc tcaaaaataa
    19741 tcagacgatt aaccgggatc tcacacagtc tttttagcgt gatagtcagc ggaacaacaa
    19801 taaacagcgc gacatcgttg gtcagaaagg tagaaagcag cgccgccgcc agcaccataa
    19861 acatcgccag ccgacgctcc gtagcaaagc ggcgcaccat tttgcgcccc agcacatcaa
    19921 aataaccgct taactccaca cctttggtca gcagcatcag gccgcttaag gtgatgatgg
    19981 tgtgccagtc gatagcagca ggccaggatt tcggtgcaaa gggcacgaaa aagcttaatc
    20041 cgataccaac aagaattaat aactgaaaaa aacgatcgcc ttgcagcgtg cgtaaaaaag
    20101 gcaggctcat ttggtaccgt gtttctgggt aaacaaacga aaagcgtcga gcgtctcttc
    20161 actaacatgg tgctccatgc cttccgcgtc gcgacgggcg atttccggac tgacgcccaa
    20221 caccaacaag aaattttcga ctatctgatg tcgctcgcgg ctttcctgcg ccagcttctc
    20281 tccttctgcc gttaaaaaca cgccacgcca ggggatcatt tcaatcagcc ccatggttgc
    20341 cagccgctta agcattttag ccaccgtcgg ttgcgaaact cccagacgag cagccatgtc
    20401 cacctgacga gcttccccca cttccctgat caagtcagaa atcagctcaa cgtaatcatc
    20461 aataagctcg cgccgatgcg cctctctgac ctggcggaac ccttcaacgt gctcttccac
    20521 gttcactaac tgcgtcactt tttttgctgt tggcgtacct gcgcgacgac tcattgtgct
    20581 tcctcattat ggtgacgcat acatagcgtt ctcatttcgt gcgcacattg taaaccagag
    20641 ttgcgaaggt acaaaaaatt aacgttttag caatagctat ataatatagc ctgtgctata
    20701 tctgtatgta atgcaatcat ccctcaagga tcgacgggat tagcaagtca ggaggtctta
    20761 tgaatgagtt caagaggtgt atgcgcgtgt ttagtcattc tccctttaaa gtacggttaa
    20821 tactgctctc tatgttgtgc gatatggtca acaacaaacc gcagcaagat aaaccttccg
    20881 ataaatagcg gcgtcgcggt acgccgcttc actcctgctt tcatgcaggc ataacgcgtt
    20941 ttggcctgaa aaaccccact ttttgtcgga tttgcaatcc ccttcgcaaa agatttgttc
    21001 gtcagtagtt gacctgaacg gcggctcgct ctatcttctt gcagccctgc gtatattgcg
    21061 gctcgcggat gcggacccct ttccactctt cacgcactct tgcaggtatt gacccttgac
    21121 gccagggtaa gcacatggcg tttgttacga tagtggcata tgaatttaac cctcaaagaa
    21181 tcgcttgtta cccgtagccg ggtatttagc ccgtggactg cgttctactt tttacagtcg
    21241 ctattaatta acctcggctt aggttacccc ttcagtttgc tctacaccgc tgcgtttacg
    21301 gctattttgc ttttgctatg gcgaacattg cctcgcgtac aaaaagttct ggtcggtgtc
    21361 agttcgctgg tggcggcttg ttatttccct tttgctcagg cctacggcgc gcctaatttc
    21421 aatacattgc tggcattgca ctccaccaat atggaagagt cgaccgaaat cctgacgatt
    21481 tttccgtggt acagctacct ggtcggctta tttatttttg cgctcggcgt aatagcaatc
    21541 aggcgaaaaa aagagaatga aaaagcgcgc tggaatacct tcgacagcct gtgtctggta
    21601 ttcagtgtgg cgacattttt tgttgctccc gtgcaaaacc tggcctgggg tggcgtattt
    21661 aaactgaaag atactggcta tccggtattt cgttttgcta aggatgtcat cgtcaataat
    21721 aacgaggtga ttgaagagca agaacggatg gcaaaacttt ccggaatgaa agatacctgg
    21781 acggtcactg ccgttaagcc gaagtatcag acctatgtgg tggtgatcgg tgaaagcgcg
    21841 cgtcgcgatg ccctcggtgc ctttggcggt cactgggaca ataccccgtt tgccagcagc
    21901 gttaacggtt tgatatttgc tgactacatt gccgccagtg gctccacgca gaaatcgctt
    21961 ggcttaacgc tcaatcgcgt agtcgatggc aaaccacagt ttcaggataa ctttgtcacc
    22021 ctggcaaatc gcgcgggctt ccagacctgg tggttttcca accagggcca aatcggcgaa
    22081 tacgatactg ctatcgccag catcgccaaa cgtgcagatg aagtgtactt cctgaaagaa
    22141 ggtaattttg aagcagataa aaacacgaaa gacgaagcgt tactggatat gaccgctcaa
    22201 gtgctggcgc aagagcactc gcaaccgcag ctgattgttc tgcatctgat gggctcgcat
    22261 ccgcaggcct gcgacaggac acaaggcaaa tacgaaacct ttgtgcaatc gaaagaaacg
    22321 tcgtgctatc tctataccat gacgcaaacg gacgatttac tgcgcaagct gtacgatcag
    22381 ttacgcaaca gcggcagcag cttctctctg gtttactttt ctgaccacgg tctggccttt
    22441 aaagagcgcg gcaaagacgt gcaatacctt gcccatgatg ataaatatca gcaaaatttc
    22501 caggtgcctt ttatggtcat ttccagcgac gataaagcgc atcgggtgat taaagcccgc
    22561 cgctcagcca atgacttctt aggctttttc tcgcagtgga cgggaattaa agcgaaggaa
    22621 ataaatatca aatacccgtt tatatctgag aagaaagccg ggccgatata catcaccaac
    22681 ttccagttac agaaggtgga ttacaaccat ctcggaaccg atattttcga cccgaaacct
    22741 taaaaacaaa aatccgcccc gagaggcgga ttttttatat caccaaagtg attagaagcg
    22801 gtaaccaaca ccggcaatcc aggtgcctac gtcaacgcta cgaatacggc tctgctcgta
    22861 agagaagtcc agagcaacgt tttccatcgg gttgaactgc agaccagcac cgtaggagaa
    22921 accgtagtcg ctggtgtcgt gtttgtaggt cgggtattca gtggtctgga atttaccata
    22981 acccacaccc actacaccgt agatgcttgc ccagtcgtta atgcggtaag ccggaccagc
    23041 agtgatgccg tagtactggt ttttgttgta gtcaccagag cttgcagtac gacttttctc
    23101 ggtgtaagtg aaagaaccga tcacacccag cgggctgttg tcttcttcat agcggtattt
    23161 caggttgaaa ccgcccattt tgttcatttg gccctgagcg tcgctctgtg cgtaaccgcc
    23221 agttacggta gaagtcgcag ctacggaagt acctgcggtg aaagccagaa ctgcggccag
    23281 tgctgaaaga catgcaattt ttttcataac cacctcaaat gtgattcaaa taagtcctaa
    23341 gttttaaata tatcaaaaat taatgggaaa ctcttcgcga tttgtgatgt ctaacgggcc
    23401 atttcatgta acagaacgtt tccatacacc gctatccatc taaatttaaa tcactttttc
    23461 agagaactgc gtaagtatta cgcatgtttt ccctgtcatt catccagatt attcctaatc
    23521 accagactaa tgattccatc aatcctggcg cattttagtc aaaacggggg aaaatttttt
    23581 caacaaatgc tcaaccagca ttgggtatat ccagtacact ccacgcttta cttaagtcta
    23641 gatatttgtg ggagaaagga tgcctggttc attacgtaaa atgccggtct ggttaccaat
    23701 agtcatattg ctcgttgcca tggcgtctat tcagggtgga gcctcgttag ctaagtcact
    23761 ttttcctctg gtgggcgcac cgggtgtcac tgcgctgcgt ctggcattag gaacgctgat
    23821 cctcatcgcg ttctttaagc catggcgact gcgctttgcc aaagagcaac ggttaccgct
    23881 gttgttttac ggcgtttcgc tgggtgggat gaattatctt ttttatcttt ctattcagac
    23941 agtaccgctg ggtattgcgg tggcgctgga gttcaccgga ccactggcgg tggcgctgtt
    24001 ctcttctcgt cgcccggtag atttcgtctg ggttgtgctg gcggttcttg gtctgtggtt
    24061 cctgctaccg ctggggcaag acgtttccca tgtcgattta accggctgtg cgctggcact
    24121 gggggccggg gcttgttggg ctatttacat tttaagtggg caacgcgcag gagcggaaca
    24181 tggccctgcg acggtggcaa ttggttcgtt gattgcagcg ttaattttcg tgccaattgg
    24241 agcgcttcag gctggtgaag cactctggca ctggtcggtt attccattgg gtctggctgt
    24301 cgctattctc tcgaccgctc tgccttattc gctggaaatg attgccctca cccgtttgcc
    24361 aacacggaca tttggtacgc tgatgagcat ggaaccggcg ctggctgccg tttccgggat
    24421 gattttcctc ggagaaacac tgacacccat acagctgctg gcgctcggcg ctatcatcgc
    24481 cgcttcaatg gggtctacgc tgacagtacg caaagagagc aaaataaaag aattagacat
    24541 taattaaatt tacatttctg catggttatg cataaccatg cagaatttct cgctactttt
    24601 cctctacacc gtctttatat atcgaattat gcaaaagcat atttattccg aaaattcctg
    24661 gcgagcagat aaataagaat tgttcttatc aatatatcta actcattgaa tctttattag
    24721 ttttgttttt cacgcttgtt accactatta gtgtgatagg aacagccaga atagcggaac
    24781 acatagccgg tgctatactt agtctcgtta attactggga cataacatca agaggatatg
    24841 aaattatgag taccgctaaa ttagttaaat caaaagcgac caatctgctt tatacccgca
    24901 acgatgtctc cgacagcgag aaaaaagcaa cagtagagtt gctgaatcgc caggttatcc
    24961 agtttattga tctttctttg attaccaaac aagcgcactg gaacatgcgc ggcgctaact
    25021 tcattgccgt acatgaaatg ctggatggct tccgcaccgc actgatcgat catctggata
    25081 ccatggcaga acgtgcagtg cagctgggcg gtgtagctct ggggaccact caagttatca
    25141 acagcaaaac cccgctgaaa agttacccgc tggacatcca caacgttcag gatcacctga
    25201 aagaactggc tgaccgttac gcaatcgtcg ctaatgacgt acgcaaagcg attggcgaag
    25261 cgaaagatga cgacaccgca gatatcctga ccgccgcgtc tcgcgacctg gataaattcc
    25321 tgtggtttat cgagtctaac atcgaataaa tccatcgctg atggtgcaga actttagtac
    25381 ccgataaaag cggcttcctg acaggaggcc gttttgtttt gcagcccacc tcaacgcact
    25441 tatttagtgc atccatctgc tatctccagc tgattaagta aattttttgt atccacatca
    25501 tcacacaatc gttacataaa gattgttttt tcatcaggtt ttacgctaaa taatcactgt
    25561 gttgagtgca caattttagc gcaccagatt ggtgccccag aatggtgcat cttcagggta
    25621 ttgccctata aatcgtgcat cacgtttttg ccgcatctct aaaaatcaat gagttgcaaa
    25681 actggcacga ttttttcata tatgtgaatg tcacgcaggg gatcgtcccg tggatagaaa
    25741 aaaggaaatg ctatgaagtc tgtattaaaa gtttcactgg ctgcactgac cctggttttt
    25801 gcggtttctt ctcatgccgc ggataaaaaa ttagttgtcg cgacggatac cgccttcgtt
    25861 ccgtttgaat ttaaacaggg cgataaatat gtgggctttg acgttgatct gtgggctgcc
    25921 atcgctaaag agctgaagct ggattacgaa ctgaagccga tggatttcag tgggatcatt
    25981 ccggcactgc aaaccaaaaa cgtcgatctg gcgctggcgg gcattaccat caccgacgag
    26041 cgtaaaaaag cgatcgattt ctctgacggc tactacaaaa gcggcctgtt agtgatggtg
    26101 aaagctaaca ataacgatgt gaaaagcgtg aaagatctcg acgggaaagt ggttgctgtg
    26161 aagagcggta ctggctccgt tgattacgcg aaagcaaaca tcaaaactaa agatctgcgt
    26221 cagttcccga acatcgataa cgcctatatg gaactgggca ccaaccgcgc agacgccgtt
    26281 ctgcacgata cgccaaacat tctgtacttc atcaaaaccg ccggtaacgg tcagttcaaa
    26341 gcggtaggtg actctctgga agcgcagcaa tacggtattg cgttcccgaa aggtagcgac
    26401 gagctgcgtg acaaagtcaa cggcgcgttg aaaaccctgc gcgagaacgg aacttacaac
    26461 gaaatctaca aaaaatggtt cggtactgaa ccgaaataat aacgctacac ctgtaaagcg
    26521 cactggcagt tccctctccc ctctggggag aggattaggg tgaggggcgc aaacccgctc
    26581 cggggccatt aattaccctg aatttgatta tttacaccac ggtaacagga acaacatatg
    26641 cagtttgact ggagtgccat ctggcctgcc attccgcttc tgattgaagg tgccaaaatg
    26701 accctgtgga tttcggtcct cggtctggct ggcggtctgg taatcggatt gctggcaggt
    26761 tttgcacgca ccttcggtgg ttggatagcc aaccacgtcg cgctggtctt tattgaagtg
    26821 atccgcggca cacctatcgt cgtccaggtg atgtttatct atttcgccct gccgatggcg
    26881 tttaacgact tacgcatcga cccatttact gcggcggtgg tcaccatcat gatcaactcc
    26941 ggcgcgtaca ttgcggaaat cacgcgtggt gcggtgctgt ctattcacaa aggttttcgt
    27001 gaagcaggac tggcgctcgg tctttcacgt tgggaaacca ttcgctacgt cattttaccg
    27061 ctggcactgc gtcgtatgct gccgccgctg ggtaaccagt ggatcatcag cattaaagac
    27121 acctcgctgt ttattgttat cggcgtggcg gaactgaccc gtcaggggca agaaattatt
    27181 gccggtaact tccgcgccct tgagatctgg agcgccgtgg cggtgttcta tctgattatt
    27241 accctggtgc tgagctttat tctgcgtcgt ctggaaagaa ggatgaaaat cctgtgattg
    27301 aatttaaaaa cgtctccaag cactttggcc caacccaggt gctgcacaat atcgatttga
    27361 acattgccca gggcgaagtc gtggtgatta tcgggccgtc cggttccggt aaatcgaccc
    27421 tgctgcgctg catcaacaaa ctggaagaaa tcacctccgg cgatctgatt gtcgatggcc
    27481 tgaaggttaa cgatccgaaa gttgacgagc gcctgattcg ccaggaagca ggtatggtgt
    27541 tccagcagtt ttacctcttc ccgcatctga cggcgctgga aaacgtcatg tttggtccgc
    27601 tacgcgtgcg tggcgcgaac aaagaagagg cggaaaaact ggcgcgtgag ctgctggcga
    27661 aagtcggtct ggcagaacgt gcacatcact acccttccga actttctggt ggtcaacagc
    27721 agcgtgtggc gattgcccgc gcgctggcgg tgaagccgaa aatgatgctg tttgatgaac
    27781 cgacttccgc tcttgacccg gaactgcgcc atgaagtgct gaaggttatg caggatctgg
    27841 ctgaagaagg gatgacgatg gtgatcgtga cccacgaaat cggttttgcc gagaaagtag
    27901 cttcgcggct gatctttatc gacaaaggcc ggattgcgga agatggcaat ccgcaggtgt
    27961 tgatcaagaa cccgccgagc cagcgcttgc aggaattttt gcagcacgtc tcttaataag
    28021 acacattgcc tgatgcgcta cgcttatcag gcctacagga tatctggcaa cttattaaaa
    28081 ttgcatgaac ttgtaggacg gataaggcgt tcacgccgca tccggcaaaa aagcccgcac
    28141 gttgtcagca atctgcttaa tatcccttcc tccctttcac ccgaaaggga ggcacaccag
    28201 attcctctca tttaaaatcg cccc
//
LOCUS       DADLFA010000033        27850 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000033 DADLFA010000000
VERSION     DADLFA010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27850
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.033"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..527
                     /locus_tag="JQH65_001228"
     CDS             <1..527
                     /locus_tag="JQH65_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HAY6138708.1"
                     /translation="KYKIMANHHGIIFDEQRKRYIIGSITVSIDEMTNALDPVELAEI
                     INKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLEALLACSQMLGINQVVAHN
                     GNGGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGAGPEITTPWKSYQHEKNRN
                     GLMPLIRRRTMLQL"
     gene            893..1960
                     /locus_tag="JQH65_001229"
     CDS             893..1960
                     /locus_tag="JQH65_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY6138709.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHMRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            1957..4692
                     /gene="rbbA"
                     /locus_tag="JQH65_001230"
     CDS             1957..4692
                     /gene="rbbA"
                     /locus_tag="JQH65_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HAY6138710.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMSAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSTLSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDMWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            4692..5816
                     /locus_tag="JQH65_001231"
     CDS             4692..5816
                     /locus_tag="JQH65_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HAY6138711.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            5889..6164
                     /locus_tag="JQH65_001232"
     CDS             5889..6164
                     /locus_tag="JQH65_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAY6138712.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            6161..6520
                     /locus_tag="JQH65_001233"
     CDS             6161..6520
                     /locus_tag="JQH65_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HAY6138713.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLLEYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(6640..7041)
                     /gene="nikR"
                     /locus_tag="JQH65_001234"
     CDS             complement(6640..7041)
                     /gene="nikR"
                     /locus_tag="JQH65_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HAY6138714.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(7047..7853)
                     /gene="nikE"
                     /locus_tag="JQH65_001235"
     CDS             complement(7047..7853)
                     /gene="nikE"
                     /locus_tag="JQH65_001235"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HAY6138715.1"
                     /translation="MTLLNVSGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDETVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRATEKV"
     gene            complement(7850..8614)
                     /gene="nikD"
                     /locus_tag="JQH65_001236"
     CDS             complement(7850..8614)
                     /gene="nikD"
                     /locus_tag="JQH65_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HAY6138716.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKAVYPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(8614..9447)
                     /gene="nikC"
                     /locus_tag="JQH65_001237"
     CDS             complement(8614..9447)
                     /gene="nikC"
                     /locus_tag="JQH65_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HAY6138717.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSH
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVSGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(9444..10388)
                     /gene="nikB"
                     /locus_tag="JQH65_001238"
     CDS             complement(9444..10388)
                     /gene="nikB"
                     /locus_tag="JQH65_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HAY6138718.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(10388..11962)
                     /gene="nikA"
                     /locus_tag="JQH65_001239"
     CDS             complement(10388..11962)
                     /gene="nikA"
                     /locus_tag="JQH65_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HAY6138719.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKLSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKTPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(12073..12660)
                     /gene="acpT"
                     /locus_tag="JQH65_001240"
     CDS             complement(12073..12660)
                     /gene="acpT"
                     /locus_tag="JQH65_001240"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HAY6138720.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(12715..13751)
                     /locus_tag="JQH65_001241"
                     /pseudo
     CDS             complement(12715..13751)
                     /locus_tag="JQH65_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
     gene            13883..15100
                     /locus_tag="JQH65_001242"
     CDS             13883..15100
                     /locus_tag="JQH65_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY6138721.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKSWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(15104..15661)
                     /gene="dcrB"
                     /locus_tag="JQH65_001243"
     CDS             complement(15104..15661)
                     /gene="dcrB"
                     /locus_tag="JQH65_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HAY6138722.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAENNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(15734..16399)
                     /locus_tag="JQH65_001244"
     CDS             complement(15734..16399)
                     /locus_tag="JQH65_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HAY6138723.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSILGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            16620..16865
                     /gene="tusA"
                     /locus_tag="JQH65_001245"
     CDS             16620..16865
                     /gene="tusA"
                     /locus_tag="JQH65_001245"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HAY6138724.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(16967..19165)
                     /gene="zntA"
                     /locus_tag="JQH65_001246"
     CDS             complement(16967..19165)
                     /gene="zntA"
                     /locus_tag="JQH65_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HAY6138725.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIAHQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQSATHPLAQAIVREAQVAALAIPAAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEYKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(19239..19865)
                     /locus_tag="JQH65_001247"
     CDS             complement(19239..19865)
                     /locus_tag="JQH65_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HAY6138726.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYF"
     gene            20006..20365
                     /locus_tag="JQH65_001248"
     CDS             20006..20365
                     /locus_tag="JQH65_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HAY6138727.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(20368..20637)
                     /locus_tag="JQH65_001249"
     CDS             complement(20368..20637)
                     /locus_tag="JQH65_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HAY6138728.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(20627..21223)
                     /gene="rsmD"
                     /locus_tag="JQH65_001250"
     CDS             complement(20627..21223)
                     /gene="rsmD"
                     /locus_tag="JQH65_001250"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HAY6138729.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            21373..22866
                     /gene="ftsY"
                     /locus_tag="JQH65_001251"
     CDS             21373..22866
                     /gene="ftsY"
                     /locus_tag="JQH65_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAY6138730.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPVEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLF
                     RGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMG
                     EILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFELQGKSVMLAAGDTFRA
                     AAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSH
                     LMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDG
                     TAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            22869..23537
                     /gene="ftsE"
                     /locus_tag="JQH65_001252"
     CDS             22869..23537
                     /gene="ftsE"
                     /locus_tag="JQH65_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAY6138731.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            23530..24588
                     /gene="ftsX"
                     /locus_tag="JQH65_001253"
     CDS             23530..24588
                     /gene="ftsX"
                     /locus_tag="JQH65_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HAY6138732.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTNFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            24833..25687
                     /gene="rpoH"
                     /locus_tag="JQH65_001254"
     CDS             24833..25687
                     /gene="rpoH"
                     /locus_tag="JQH65_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007899894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HAY6138733.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            25958..27061
                     /locus_tag="JQH65_001255"
     CDS             25958..27061
                     /locus_tag="JQH65_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HAY6138734.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(27260..27643)
                     /gene="panM"
                     /locus_tag="JQH65_001256"
     CDS             complement(27260..27643)
                     /gene="panM"
                     /locus_tag="JQH65_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HAY6138735.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         6593 a         7228 c         7741 g         6288 t
ORIGIN      
        1 ccaaatacaa aataatggca aatcatcatg ggataatttt tgatgagcag cgaaaacgtt
       61 acattatagg atcgataaca gtctctattg atgaaatgac caatgctctc gatccggtcg
      121 aattagcgga aattattaat aaaaagcacc atgctgtaat taatggcaaa aagtttaaaa
      181 atagccgggc tatctcttgt cgatccttta atcgttattt ttctgtttcg acagattaca
      241 gacctaaact tgaagcacta ttagcatgct cacaaatgtt aggtataaat caagttgttg
      301 cacataacgg taatggtgga agagaatgta taggtgaaac ggggacagtt ttaggtctta
      361 acgcccgtga cagtaaacat gctggaagaa tgttcagcat gcataattgc caaattaacc
      421 ctggtgcagg gcctgagata accactccct ggaagtctta ccagcatgaa aaaaacagaa
      481 atggacttat gcctttgatt agaaggcgca caatgttaca gctctgaacc atagacaaca
      541 tgaatattaa tgttactatc tggttgagac tgttgataaa acgtaaaaag gaatgctctg
      601 ttcctgcaac tgaacaggcg tgaaacgcga tccaacagga tggataccgt tttgtcagcc
      661 agttttatag atcacactta tgagtatgcg tgtttcattt acggccagga atgctgatga
      721 tgacaacatt actacccgtt ttcactaagc cttccccgtt ggcgctcaat gctctgcgcg
      781 ctggtcgtat ttgtcgtttc cttcttatcc cggatggaag aatccgctga acgtcgttcg
      841 gcggcagttg taacacgcgt cgtttgctta cagggattat ggcgggccga ttatggataa
      901 gagtaagcgc catctggcgt ggtgggttgt cggggcactg gcggtggctg ccgtcgtggc
      961 gtggtggctg ttgcgcccgg cgggtgtgcc ggaaggcttt gctgtcagca atgggcgcat
     1021 tgaagcgacg gaagtggata tcgccagcaa aattgccggg cgtatcgaca ccattctggt
     1081 gaaagaaggc cagtttgttc gcgaaggtga agtgctggcg aagatggata ctcgtgtgtt
     1141 gcaggaacag cgactggaag ccatcgcgca aatcaaagag gcacaaagcg ccgttgccgc
     1201 cgcgcaggct ttgctggagc aacgacaaag cgaaactcgc gccgcgcagt cgctggttaa
     1261 tcaacgccag gcagaactgg actccgtagc aaaacgtcat atgcgttccc gttcactggc
     1321 ccaacgaggg gctatttctg cgcaacagct ggatgacgac cgcgccgccg ctgagagcgc
     1381 ccgagctgcg ctggaatcgg caaaagctca ggtttctgct tctaaagccg ctatagaggc
     1441 ggcacgtacc aatatcattc aggcgcaaac ccgcgtcgaa gcggcacaag ccactgaacg
     1501 gcgcattgcc gcagatatcg atgacagcga actgaaagcc ccgcgtgacg gacgcgtgca
     1561 gtatcgggtt gccgagccag gggaagtgct ggcggcaggc ggtcgggtgc tgaatatggt
     1621 cgatctcagc gacgtctata tgactttctt cctgccaacc gaacaggcgg gcacgctaaa
     1681 actgggcggt gaagcacggc tgatcctcga tgccgcgcca gatctgcgta ttccggcgac
     1741 catcagtttt gtcgccagcg tcgcccagtt cacgccaaaa accgtcgaaa ccagcgatga
     1801 acggctgaaa ctgatgttcc gcgtcaaagc gcgtatccca ccggaattac tccagcagca
     1861 tctggaatat gtcaaaacgg gcttgccggg cgtagcgtgg gtgcgggtga atgaagaact
     1921 tccgtggcct gacgacctgg tggtgaggtt gccgcaatga cgcatctgga actggttccc
     1981 gtcccgcctg tcgcgcaact ggcgggcgtg agccagcatt atggaaaaac cgttgcgctg
     2041 aacaatatca ctctcgatat tccggcccgc tgtatggtcg ggctgattgg cccggacggc
     2101 gtcgggaagt cgagtttgtt gtcgttgatt tccggtgctc gtgtcattga gcagggcaat
     2161 gtgatggtgc tgggcggcga tatgcgcgac ccgaagcatc gccgcgacgt ctgcccgcgc
     2221 atcgcctgga tgccgcaggg gctgggcaaa aacctctacc acaccttgtc ggtgtatgaa
     2281 aacgtcgatt ttttcgcccg cctgttcggt cacgacaaag cggagcggga agtgcgaatt
     2341 aatgagctgc tgaccagcac ggggttagca ccgtttcgcg atcgtccggc agggaaactc
     2401 tccggcggga tgaagcaaaa acttgggctg tgctgcgcgt taatccacga cccggaactg
     2461 ttgatccttg atgagccaac aacgggggtt gacccgctct cccgcgccca gttctgggat
     2521 ctgatcgaca gtattcgcca gcggcagagc aatatgagcg tgctggtcgc caccgcctat
     2581 atggaagagg ccgaacgctt cgactggctg gtagcgatga atgccggaga agtgctggca
     2641 accggcagcg ccgaagagct gcggcagcaa acgcaaagcg ccacgctgga agaagcattt
     2701 attaatctgt taccgcaagc gcaacgccag gcgcatcagg cggtagtgat cccaccgtat
     2761 caacctgaaa acgcagagat tgccatcgaa gcgcgcgatc tgaccatgcg ttttggttcc
     2821 ttcgttgccg ttgatcacgt caatttccgc attccacgcg gggagatttt tggattcctt
     2881 ggttcgaacg gctgcggtaa atccaccacc atgaaaatgc ttaccggact gctgcccgcc
     2941 agcgaaggtg aggcgtggct atttgggcaa ccggttgatc caaaagatat cgatacccgc
     3001 cgtcgtgtgg gctatatgtc gcaggcgttt tcgctctata acgaactcac cgtgcggcaa
     3061 aacctggagt tacatgcccg tttgtttcac atcccggaag cggaaattcc cgcgcgagtg
     3121 gctgaaatga gcgagcgttt taagctcaac gacgttgaag atgttctgcc ggagtcattg
     3181 ccgctcggca ttcgccagcg gctttcgctg gcggtggcgg tgattcatcg cccggagatg
     3241 ttaatcctcg atgagcctac ttctggtgtc gatccggtgg cgagggatat gttctggcag
     3301 ttgatggtcg atctctcgcg ccaggacaaa gtgactatct tcatctccac ccactttatg
     3361 aacgaagcgg aacgttgcga ccgcatctca ctgatgcacg ccggaaaagt gcttgccagc
     3421 ggtacaccgc aggaactggt tgagaaacgc ggagccgcca gtctggaaga ggcatttatc
     3481 gcctatttgc aggaagcggc agggcagagc aacgaagccg aagcgccgcc cgtggtacac
     3541 gacaccaccc acgcgccgcg tcagggattt agcctgcgcc gtctgtttag ctacagccgc
     3601 cgcgaagcgc tggaactgcg acgcgatcca gtacgttcga cgctggcgct gatgggaacg
     3661 gtgatcctga tgctgataat gggttacggc atcagtatgg atgtggaaaa cctgcgcttt
     3721 gcggtgctcg accgcgacca gaccgtcagt agccaggcgt ggacactcaa cctctccggt
     3781 tcccgttact ttatcgaaca gccgccgctc accagttatg acgagcttga tcgtcggatg
     3841 agtgcgggcg atatcacggt ggcgattgag atcccgccca atttcgggcg cgatatcgcg
     3901 cgtggtacgc ctgtggaact cggcgtctgg atcgacggag cgatgcccag tcgtgccgaa
     3961 acggtaaaag gttacgtgca ggcaatgcac cagagttggc ttcaggatgt ggcgagccga
     4021 caatcgacac ccgccagcca aagcgggctg atgaacattg agacgcgtta tcgctataac
     4081 ccggacgtaa aaagcctgcc agcaattgtt ccggcggtga tcccgcttct gctgatgatg
     4141 atcccgtcaa tgctaagcac ccttagcgtg gtgcgggaaa aagagcttgg gtcgattatc
     4201 aacctttacg tgacccccac cacgcgtagt gaatttttgc ttggtaaaca gttgccatac
     4261 atcgcgctgg ggatgctgaa ctttttcctg ctctgcggcc tgtcggtgtt tgtgtttggc
     4321 gtaccgcata aaggcagttt cctgacgctc accctggcgg cgctgctgta tatcatcatt
     4381 gccaccggaa tggggctgct gatctccacc tttatgaaaa gccagattgc cgccattttc
     4441 ggaacggcga ttatcacgtt gatcccggcg acacagtttt ccgggatgat cgatccggta
     4501 gcttcgctgg aagggcctgg acgttggatc ggcgaggttt acccgaccag tcattttctg
     4561 actatcgccc gcgggacgtt ctcgaaagcg ctggatctga ctgatatgtg gcaacttttt
     4621 atcccgttgc tgatagccat cccgctggtg atgggcttaa gtatcctgct gctgaaaaaa
     4681 caggagggat gatgcgccat ttacgcaata tttttaatct gggtatcaaa gagttgcgca
     4741 gtctgctcgg tgataaagcg atgctgacgc tgattgtctt ctcgtttacg gtgtcggtgt
     4801 attcgtcagc gaccgttacg ccaggatcgt tgaacctcgc gccgatcgcc attgccgata
     4861 tggatcaatc gcagttatcg aaccggatcg ttaacagctt ctatcgtccg tggtttttgc
     4921 caccggagat gatcaccgcc gatgagatgg atgccggact ggacgccgga cgctatacct
     4981 tcgcgataaa tattccgcct aattttcagc gtgatgtcct cgccggacgc cagccggata
     5041 ttcaggtgaa cgtcgatgcc acgcgcatga gccaggcatt taccggcaat gggtatatcc
     5101 agaatattat caacggtgaa gtgaacagct ttgtcgcgcg ctaccgtgat aacagcgaac
     5161 cgttggtatc gctggaaacc cggatgcgct ttaacccgaa ccttgatccc gcgtggtttg
     5221 gcggggtgat ggcgatcatc aacaacatta ccatgctggc gattgtattg accggatcgg
     5281 cgctgatccg cgagcgtgaa cacggcacgg tggaacactt actggtgatg ccgataacgc
     5341 cgtttgagat catgatggcg aagatctggt cgatggggct ggtggtgctg gtggtatcgg
     5401 gattatcgct ggtgctgatg gtgaaaggtg tactgggcgt accgattgaa ggctcgatcc
     5461 cgctgtttat gctgggcgtg gcgctcagtc tgtttgccac cacgtcaatc ggcattttta
     5521 tggggacgat agcgcgttca atgccgcaac tggggctgct ggtgattctg gtgctgctgc
     5581 cgctgcaaat gctttccggt ggatccacgc cgcgcgaaag tatgccgcag atggtgcagg
     5641 acattatgct gaccatgccg acgacacact ttgttagcct cgcgcaggcc attctctacc
     5701 ggggtgccgg attcgaaatc gtctggccgc agttcctgac gctgatggca attggcggcg
     5761 cgtttttcac cattgcgctg ctgcgattca ggaagacgat tgggacaatg gcgtaaggaa
     5821 gggaaacttg acttcatact gtgatgtcat actattgatg tcataaagtg aagtcataag
     5881 gacatgccat gagaaccaag gtacaggctt tacggaagaa acaaaaaaat acattggatc
     5941 aaatatttaa aactcctgtt cctcaaggga tcaagtggtc tgatatagag tcactggtta
     6001 aagcattagg cggagaaatt aaggaaggaa gaggttcgcg ttgtaagttc atactaaata
     6061 tgagcgttgc gtgtttccat cggcctcatc cgtcgccaga taccgataaa ggcgctgtag
     6121 aaagcgtgcg tgactggtta ctaagtatag gggtaaaacc atgatcaaac tgaagacgcc
     6181 aaactctatg gagattgccg ggcaaccagc tgttattacc tacgtgccag aactgaatgc
     6241 atttcgtggc aaattcctgg gcttgtcagg ttattgtgat tttgtctctg acagcattca
     6301 ggggctgcaa aaagaagggg aactttccct gctagagtat cttgaggatt gtaaagctgc
     6361 gggtattgag ccgtatgcgc gtacagaaaa gattaagacc tttacgttac gctatccaga
     6421 atcgctgagt gaacgcctta ataatgcggc tgcgcagcaa caagtctctg ttaataccta
     6481 tattattgaa acactgaatg agcgtttgaa tcatttataa attttagcgg cgtacattac
     6541 gcagaatatc tgcctttaca ggatctatct atgcaactga acttaatttt ataaattaag
     6601 ttcagttaat atcttttctt tcctcgtttc agaaataact caatcttcct tcggcaagca
     6661 ctgcaaatgc ccatgccgca cgccgcgctg ggcgataacg tcatcggcaa aatgctgcac
     6721 gtcgcccata tcacctttca gcacggcgat ttccagacag tcgtcgtgat tgatatgcac
     6781 atgcagcgtg gcgacggaga gatcgtggtg atggtgctgg gtggagacaa tgcggctggc
     6841 taagtcgcgt ttttcgtgtt catacacata ggagagcacc gcgaaacctt gcgtgccgtg
     6901 ctgctgagtg gcctcctggg ccagggcgct acgcagaata tcgcggatag cttcggaacg
     6961 gttgttataa ccacgacgct ggctcaggct gtccagcgtc tccagtaaat cgtcatcaag
     7021 cgtgatggtg actcgttgca tttgcgttaa accttttctg tggcgcgacg gcgcacgggg
     7081 aatgcgggta ataccgcgtt ttgtagcaca cgtccggcgt cagaggaaaa ggttaattta
     7141 tctcccaccg cctgggtttc gacgatttgt ccgttgtcca tcaccatcac ccgctggcaa
     7201 aagcgttcta caagacgtaa gtcgtgggtg atgaacaggc aggcggtgcc aaactgttgt
     7261 tgcagctttt tcagcaggcg aatgacgccc gcctgtaaca cgagatcaag gttagaaacg
     7321 gtttcatcca gaatcagcaa tttcggttcg accgccagcg cgcgtgccag gcagacgcgc
     7381 tggagctggc cgccgcttaa ctgcggtggg cgtttgtcga gaacgctgtc atcgagatcg
     7441 accgccttca gcatttcgct ggcgcgcgcc agttgttcgg attttttcag tgatagcagg
     7501 tggcgcatcg gttcacgcag gatctcgcgc acggttttgc gcggattcac ggcactgatg
     7561 gagtcctgaa acaccatctg aatatcacgg cggaaggctt tacgttgggc gcgattaagt
     7621 ttcgccagcg attcgccgcg ccagctaata ttcccttgtg agggagactc taagcctacc
     7681 agcagccgcg cgagggtgct ttttccgcag ccgctgcgtc ccagcagggc gacggtttcg
     7741 ccgcttttca gggtcaggga aacgttattc agcaccgcct gatgttgatg ttttccgcta
     7801 aatccaccgt gggcatagtg atgggaaagg ccgctgacgt taagtaaggt catgatgcca
     7861 gctccatacc gtagagggcg agatgagcgg aaaccaggct gcgcgtcacc gtatgtttgg
     7921 gggcgttaaa cagcgtttct acatcgccct gttcgacaat tttaccttgt gacatcaccg
     7981 ccacgtcatc cgccagacgc gccacaacgc ccatatcatg ggtgaccagc agcattcccg
     8041 gcgcttgttt ttgcataatg ctttccagca gatcgaggat gcgcgcctgt gctaccacgt
     8101 cgaggtcggt ggtcggttca tcggcgatga taaacggtga ttcacacagc accgccatcg
     8161 caatcatcat gcgctgcaac atgccgccgc tcatctcgaa cgggtacagc ttcagcacgc
     8221 gcgcggcgtt ttccagcccc accgcttcta tggcagcggt aagcgtagcg tcatcggcgg
     8281 gtttccctaa cgccaggcag gtttcccgcg cgtgggtgtg catggtgtgt agcggattaa
     8341 aggcgctgcg cgggttctgc atgatggtgg caattttgat gccgcgcagg gcgcagggat
     8401 aaaccgcttt gccatcggct aaaatttccc ccgccgtctg gcgaacgcca gcgggcaaaa
     8461 tgcccagcgt cgcggcgcag gttaatgatt tcccgctgcc gctaccgccg actaacgcca
     8521 gcacgcgccc gcgttgcagg gttaacgata caccgtgtac cagcggctgc gcggcctgta
     8581 gcgcgatatt acgtagttca atctgttgcg gcattagtgt gcgtgctccg tcaccagatg
     8641 agggtccaga tgatcgcgca gtgcgtcacc caccaggtta aaggccatca cgctgataaa
     8701 cagcgccagc cccggccaga acatttgcag cggctgggtc cagatatact ggcgcgcgtc
     8761 gttaatcatc acgccccatt cggcggtcgg cgcggtcaca ccgaggccaa ggaaagacat
     8821 ccccgagacg tgcagcatca tatggccgat atccagcgtt gccagcacca gcagcgaagg
     8881 gatcaccgcg cctgccagat gatcgacaaa cactcgcaca tggcccgcac cggaaagccg
     8941 tgacgccagc acaaactcgc gttggcgtag tgaaatcacc aggctgcgca ccatgcgtgc
     9001 ataccacgcc cagtgcgaca gggcgatggc gataattacg ttggtcagcc cggtgccgag
     9061 cacgccaacc ataaagaacg acagaatcga ggtcgggaag gtcataaaca tatcggcgac
     9121 gcgcatggtg gcctgatcaa cgcgcccgcc aatcaacccg gcgctgccgc caataaccag
     9181 ccctaatgtc agcaccagca gcaggcaggc cattaccgaa ccgagcgaca cgcgggtcgc
     9241 tgccatcagc cgcgagaaaa tatcgcgacc taagtgatcg gtgcccagcc agtgctgcgc
     9301 atccggcgaa agcaggtgcg acggcaaatc aatcgcctgg gggtcatacg gcagccacca
     9361 ctggctggtg agcgcaatca gcgccagcag ggcgataatg atcagcgcca ggcgtaccga
     9421 ccagcgggaa gagaggaaaa agttcacgcg tgcgctcctt catgacgacg aatgcgcggg
     9481 tccagcgcgg cgttgagcaa atcgacaatc aaattacaga ccacaaaaac caccaccatc
     9541 atcagcgtaa agcactgaat caccggatag tcacggttaa aaatcgccga caccgcatag
     9601 cgcccgacgc caggccaggc aaagatgttt tcgataatca tcgtcccgcc aatcaattcg
     9661 ccaatgtgca tccccacggc ggtgatcatc ggcagcgagg cattgcgcag aatatggcgg
     9721 cgttcggtct gtttgtcatt caggccgcgt agccgcgccc aggtgacgtg acgctgaccg
     9781 gcgacgtcca gcatactggc gcgcagcagt cgcgcgttaa tcgccagcga cataaaggcg
     9841 atggaaaccg caggcaaaat gatgtgctgc cagccgccgt agcccatcgc gggtagccat
     9901 tgcagataca ccgaaaacgc catcactagc aaaaatgcca gccagaagtt gggcatcgac
     9961 acgccaagaa acgcgataaa gcgcacggcg aaatccggca aacgatcgcg gtggcgcgcc
    10021 gcccagatac cgagcggtac ggaagtgagc agaattaata ccagcgccgc acctgcaagt
    10081 tccagcgtgg cgggcaggaa gttcagcata tcgtccagca ccgggcgttg gctggcgaat
    10141 gagataccaa agtcaagatg cagcgccttc cacaaccagg tgccgtactg gacgtacagc
    10201 ggctgatcca gtccgagcat ggtgcgggta gaggccagca tctccggcgt cggcggcagg
    10261 ttagacagac gcaaataatc gagcgccggg tcgccggtac cgaggcgcag catcagaaaa
    10321 atgatcaccg aggcggcgag caccatcggg atcagcagca gaaagcggcg taatacgtaa
    10381 cgcaacatta aggtttcacc ggtttaatct gttcgaacgg aatttcggtg gcgatcggcg
    10441 cgtaggggat gttacccagc tccggttttg ataccaccat cattgagatg taactgatag
    10501 gcagataaac cgcctcgtca tgcagacggg tcagaatgtc gcgatacagt gcctgacgtt
    10561 gcgtttcgtc atgggtcgcc agcacttcgc cgatctcttt atcaatcagc ggtttgtcgg
    10621 ctaatccttg ctgtgcctgg aagtcagcgt gcgacggtac gcgcattgaa ctcaggaagg
    10681 cgtgtggatc gtatggcgcg ccccaggtgc ggtggaaaat catgccaaaa cgaccgtcgc
    10741 gctgacgagc atagatactg ctctcttctt cgccaatcag cgagacatct gcgccaatct
    10801 ggcgcatatc agcctgaatg atttccgcca tcgatttgct taacgcatcg gtgccgatga
    10861 acgacagttc aatgcgcagc ggctgaccat ttttctcgcg gatgtctttg cccgcaggca
    10921 gcgtccaacc ggctttttcc agcaacgctt tcgctttctg cggatcgtac tggcgcggtt
    10981 tcaggccgag gttggcgtag ggcacagaag gggcaaacag ggtgtcggcg acctgctggg
    11041 tgccatacaa cgcgttatca atcagcgatt ttttgtttac cgcgtaatta agagcttcac
    11101 gtactgccag ctcattggtg ggggttttgg cggtattaag cgccagcatc acggtttcga
    11161 tcggctgtga cagttgggta tggtaagccg ggttctggct aaagcgggcg aaggtatcga
    11221 gcggtaataa cccttcgttt ccgtacagca ggtcgatatc gccagtttca aacgccaccg
    11281 cgcgggtagt cgggtccggg atgacgttaa aggtgatctt tttaatcgct ggcttttcgc
    11341 cccagtagtt ttcgttacgg acgaagacat cgtactgatt cagtttcgat tcctgcaaaa
    11401 tccacggtcc ggtgccaatc ggcgctttaa ttccgttcat ggtttcatgg tttttaaact
    11461 gcgagggagc gataaaacgg aaaggacggg gcagggccag ttcttgcagg aaaggatagt
    11521 aggcgctttt cagggtaatt tgcagctctg ttttactgag tgctttaaca tcaacaatct
    11581 ggtttgccag ctccagccag gcgtgacgtt gacggttatc gagcactgcg cggaagtttt
    11641 ctgccgccgc ctcggcatcg aacggttcac cgttggataa tttcacgtca tcacgcaggg
    11701 tgaaggtcca ggttttacca tcttctgaat gagtccagct ttttgccagc cacgggatca
    11761 ccgaaccgtc tgcctgatat ttcaccaatg gttcataaac catgctctgg gcgaacatct
    11821 ggttaggcgt gtaaaggtgc gggtttagtg gcccgacatt caccggccag gcggtagtga
    11881 tttcatctgg tgcagcggca tggacgataa aagacgcaca agccagcagc gcaaatagag
    11941 tgcggcggag tgtggagagc atgataaccc caatggatta aaatagatgg cggaaataag
    12001 tatgacgatt ttaagtattc gtcatactga ttacctgttc tggatcaata gatgggcagt
    12061 cgggtgggcg gatcagttaa ctgaatcgat ccattgcaca ctgtcggcgg tgagcgtaaa
    12121 gggggtgggg gtgcagatcg caaggcttaa attttctagc tgacaatggc tgactgacag
    12181 cgaggagtga taggtgctat ctacgctgac gatttgccag gcgctgccgc cacgctgttt
    12241 aacgatggct tctttgcgcg tccagatgcg ccagaacatt tccagttgct gatcaggatg
    12301 cacggcgtcc atttcagcgt gttccccgag gctgaacacg gcgttcgcca gccagcgcca
    12361 gttggcgcgc gggcgaatca cttcgatatc gcagccgact tcgccttcat cgctcaacag
    12421 cagagcgata tcgtcaccgc tatggcttaa gttgaaccat agcggcgttt ccggcgcgaa
    12481 tgcaggtttg ccttgttcgc catagatgat ctccggtagc ggggaaagcg tgtgcgaaag
    12541 caatgcacgc cccgccagcc agcgttcgcg tcgtggacct tgcggtgctt gctcgcgtaa
    12601 acccggtggc agtggagctg cgcttaaggt cgaaactttc cccagaacta tccgatacat
    12661 atcagggcca acgtttaatg gaaaatgaaa gtgcgtatcg tatcacttgt cgcctcatcc
    12721 cggtaaccga cttttcggtc tgcctagccc cagtaaaatc gccagtttgc taccgccttt
    12781 ggtggtttcc atccagattt tacacacgct ggttaatggc accgaaagta gcatccctac
    12841 cgggccgagc agccatcccc aaattaacaa tgaaagaaat accaccatgg tggacatccc
    12901 caggcgatgg cccatcatcc gtggtcgatg accatatgga ccactaaaaa caatgcgccg
    12961 accagaatac atgcgtaaac accattaaac agcagcacct gaatcattgg cggtacggcg
    13021 gaaattaccg cgccgatatt gggcacgtag ttgagcaaaa acgccagtac tgcccacatc
    13081 agcgcaaact gtacgcccat tagcgccagc cccagccaga cgatgacacc tgtccataaa
    13141 ctgagtagcg tctttaacgc cagataatgc gaaacgcctt taagtgcgcg gtgtaatcct
    13201 gcgatgtgaa tctgtgggtt attcagcgca aaacgcattt tgtaagggac gtggcgcact
    13261 tcaaacagca taaaaactac ggtcatcacc agcaaaagca cgctcgccat tgccccggaa
    13321 agcccgatca ttagcgctgt ggtgaaggta accacttttt ccgagtccat ccgctgcagc
    13381 attcgctccg gcgacatatg caaattaaga aaaggcaaca tctcctgcaa tttaaaaagt
    13441 ttgcgcgtca gctccttatt aaacttcggc agcatagaga taaattcgtt aaacgatgcc
    13501 gccagtacgc cgactagcgc ggttagtgcg atcagcatca ccaccactac aatcgtaatg
    13561 gtaacggggc gttgtactcc ccgacgaata aaccaggtaa cgagcgggtt gaggacgatg
    13621 gcaaaaaaca gcgccagtaa cagctgcaca atgatatctg ccgctgcgtg aatgcccgcg
    13681 aggatcacta ccagcgaggc caacttgagc agaatgtgca tgcccgtttt atcgggttga
    13741 ggggtttcca ttggggcttc cttgtgactt tttgtgttaa gtgtagcggg agtcacgtca
    13801 gcaatattct gattcttact gctgattttc acgtcagcac gtggtaaaaa tgaaacactg
    13861 ttgtaaaaat gtggtgatcc tcatgcccga acccgtagcc gaacccgcgc taaacggatt
    13921 gcgcctgaat ttgcgcattg tctccattgt catgtttaac ttcgccagct acctcaccat
    13981 cgggttgccg ctcgctgtat taccgggcta tgtccatgat gtgatgggct ttagcgcttt
    14041 ctgggcagga ttggttatca gcctgcaata tttcgccacc ttgctgagcc gtcctcatgc
    14101 cggacgttac gccgatttgc tgggccccaa aaagattgtc gtcttcggtt tatgcggctg
    14161 ctttttgagc ggtctgggat atctgacggc aggattaacc gccagtctgc ccgtcatcag
    14221 cctgttatta ctttgcctgg gacgcgtgat ccttgggatt gggcaaagtt ttgccggaac
    14281 gggatcgacc ctgtggggcg ttggcgtggt tggctcgctg catatcgggc gagtgatttc
    14341 gtggaacggc attgtcactt acggggcgat ggcgatgggt gcgccgttag gcgtcgtgtt
    14401 ttatcactgg ggcggcttgc aggcgttagc gttaatcatt atgggcgtgg cgctggtggc
    14461 cattttgttg gcgatcccgc gtccgacggt aaaagccagt aaaggcaaac cgctgccgtt
    14521 tcgcgcggtg cttgggcgcg tctggctgta cggtatggca ctggcactgg cttctgccgg
    14581 atttggcgtc atcgccacct ttatcacgct gttttatgac gctaaaagtt gggacggtgc
    14641 ggctttcgcg ctgacgctgt ttagctgtgc gtttgtcggt acgcgtttgt tattccctaa
    14701 cggcattaac cgtatcggcg gcttaaacgt agcgatgatt tgctttagcg ttgagataat
    14761 cggcctgcta ctggttggcg tggcgactat gccgtggatg gcgaaaatcg gcgtcttact
    14821 ggcgggggca gggttttcgc tggtgttccc ggcattaggt gtagtggcgg taaaagcggt
    14881 tccgcagcaa aatcaggggg cggcgctggc aacttacacc gtatttatgg atttatcgct
    14941 tggcgtgacc ggaccactgg ctgggctggt gatgagctgg gcgggcgtac cggtgattta
    15001 tctggcggcg gcgggactgg tcgcaatcgc gttattactg acgtggcgat taaaaaaacg
    15061 gcctccggaa cacgtccctg aggccgcctc atcatcttaa aacttactga ataaccagcg
    15121 tattaatgat gttttctgcg gtggtctgcg ctttttgctg atcgtcagcg ggcagcgtaa
    15181 tttgcatggt cagcagttga ttacccacgt tacccagaat aacggaagag tacgccgtct
    15241 ggcctttcgc ggagataata ctgtctaact gctgcatttt gtgacctttc agctcaatgg
    15301 ctttattggt taccacttgc agctgcggat cgcggctacg ttgctgatct tccagacgct
    15361 tcgccagcac cgccagatct tctttcggat catcgcccat gatgacgatg actgctttct
    15421 gcccggtggc gtcggaccag acatgcatgt tattggcctg cgttcccagc ttaccgctct
    15481 ggtcggtcat atccgctggc agcgagaaac ttaacttgcc atcaagcagg ttgacgggat
    15541 tcccggtagc gttattttcc gcgaccgaac cctgcgccgt agcgttagtg tctttatcat
    15601 cacaggccgc aagccccata accagcaggc caattccgac atatttaacc agattgcgca
    15661 ttgacttctt cctttcgata aacggccata acggctcatt catccatctt atcacaactc
    15721 tgataacgaa cctttaactc gcctgcaaag cgttgatttc ggatttatct gccagtcttt
    15781 tcaacagcat attgagtaat acgccataca ttggcaggaa gaaaacgata ctgattaaca
    15841 ctttgaaaca gtaatcgacc agcgcgattt ccatccagtg ttcagccata aaggcatccg
    15901 ggctacgcca gaaggcaatg aagaaaaagg ccagcgtgtc gctgacgtta ccgaacagtg
    15961 tggacgctgt cggtgccagc caccagcggc gactctgacg caggcggtta aaaacgtgca
    16021 cgtcgaggat ctgtcccagc gcgtaggcca tgaaactggc ggtggcgata cgggcgacaa
    16081 acaggttgaa gtgggcgagt gcgccgaatc cctgccagga acccatatag aatagcgacg
    16141 agatgacgta ggagatcagc aacgccggga tcatcaccgc aaagatgata cgacgggcca
    16201 gcggtgcgcc aaaaatacgc acggtcaggt cggtagcaag gaaaataaac gggaagctaa
    16261 acgcgcccca ggtggtatgg aaacccaaaa tggagacggg aagctgaacc agatagttac
    16321 tggaggtgat caccagcaga tgaaataacg ataaccagaa caacgcctta tagcgttgag
    16381 tttgcgagaa aacgttcata ttgtaccttt ttgattaacc attggggtga gggaacccaa
    16441 tacgtacgac acgtctgtta ccttaacata ataacgtgct tacccttttt tcgagccgcc
    16501 gcatgatact gctttacgtt gacaatgcaa tggttaattt tcacgcaatc gttaacctgg
    16561 tttgcttacg gactcacagg gcgtaaacta gcgccgtttt tttatgtgat gagaagaaaa
    16621 tgaccgatct cttttccagc cctgaccaca cactcgacgc acttggcctg cgctgcccgg
    16681 aacccgtaat gatggtgcgc aaaactgtgc gcaatatgca gcccggcgaa acgttgctga
    16741 ttatcgccga cgatccggcc actacccgcg atattcctgg gttttgtacc tttatggaac
    16801 acgaactggt tgcgaaagag acggatggac tgccttatcg ttatttgatt cgtaaaggcg
    16861 gttgatgggg gctgattggc ttcgatgccg ccttttcccc tcaccctaac cctctcccca
    16921 gaggggcgag gggaccgatc gcgctcaatg ttgcgatcgg tttgccttat ctcctgcgca
    16981 acaatcttaa cgcattcgct gtcaccagca ccgtcgcacc cgtatctgcc agcaccgcca
    17041 gccatagtcc ggtcatccct aaaagcgtgg tgacgaggaa gatccctttc agtcccagcg
    17101 caatggtgat gttctggcgg atattggcgt gagtggcgcg tgccagttca atcatttgca
    17161 ccaggccgcg caggtggtta tgggttaatg ctgcatcagc ggtttccagc gccacgtctg
    17221 tgccactgcc cattgcaatc ccaatggcgg cagctttcat cgctggcgcg tcgttaatac
    17281 cgtcaccgac catcgccagc ggcgcatgtt gattcagctc ggtcaccgct ttgactttat
    17341 attccggcaa caggcccgct ttaaactcca gccccagctc cccggcaatt gccgccgctg
    17401 cgcgtggatt atcgccggtg aggatcaccc ctttgacgcc cagcgcgttc agttcactga
    17461 tggcagttgc agcatcggcg cgcagggtat cctgcaatgc aatgataccc agcacgtcat
    17521 cattacgtac taccagcaca accgtttgcc cggcgctttc cagttcatta atcaaaccag
    17581 caaatgcatc agcgggatgt ttcccggcag cgcatatcaa cacgcgctca ccgttaacct
    17641 gcgcttcaat gccagacccg accagcgccc gctgtgattc ggcggcggga atggcgagtg
    17701 cagcaacctg tgcttcgcgc acgatggcct gcgccagtgg atgcgtcgcg ctttgctcga
    17761 ccgccgccgc cagtgtcagc agttcagatt cactaatacc cgttgccgga tgaatcgcgg
    17821 taacgcgcgg tttaccgacg gtcagcgtac cggttttatc aaacgccacc tgagtaacac
    17881 gacctagttg ttccagcgcc gctccgcctt taatcaacgc cccacgacgc gctgctgccg
    17941 ccagcccgga ggtaatcgcc gcgggcgtgg agataactaa cgcacacggg cagccaatca
    18001 gcagcagcgt caggccttta taaatccact cctgccagct ggcggcaaac agcagcggtg
    18061 gcaccagcgt caccagcagg gcgacggcca taatcgctgg cgtataaata cggctgaaac
    18121 ggtcgataaa ccgctcaatg ggggcgcggc gctcttcggc ttcttcgatc agtttcagaa
    18181 tccggtcaat ggcgctggca cccggttctg acagcacttc cagcgttacc agacggtcta
    18241 cgctggtggc tcctgcagga actttatcgc ccgtcgcgcg ctccaccgga atggattctc
    18301 cggtcagggc gctttcatca aaactggcaa acggtgagag cagtttaccg tcggcaggca
    18361 aacgcccacc tgcggcgact tcaatcacat cgccaggccg caggctgtta atcgccacct
    18421 cttcccgctc accgttacgc aggcgcgtgg cggtttctgg tttcagcgcc attaacgcgc
    18481 taacgccctg acgcgcgcgg ctggcggccc agccttccag tcgttcacca atcaaaaaca
    18541 gcagcaacac catcgcagct tcagccgttg cgccaataaa cagtgcacca atagcggcta
    18601 cgctcattaa ggtttcaatg gcgaagtagc tgccggattt gatcagccgt aatgcctgat
    18661 gagcaatcgg gtacagccca accagcgtgg tcgcgataaa cgccagttgc ccgaacggat
    18721 gattgaactg ctccagaccc cagctgattg ccatcatcac gattagtgta atcagcggca
    18781 gattctcttt caggcgcgat gcttgtggct cgtcggcggc ctgctcatcg cgcagggaat
    18841 agcccgcttt ttgcaccgca gattcaactt gtgcacgaat gtcattgtcg gcatcaacca
    18901 ccagtttttc ggtggcgaac aacacctgca cctgattcac acctgcaagc tggcgcacgg
    18961 cattttctac cttgcgcgca caggcggcgc agtccatgcc actgactttc cagctatagc
    19021 gggtgccgga gacgttttca gagagcgttg gcgagctgga gcatgcgccg tcgcagcaac
    19081 agtcgttggc gttctgtacc gtggttagcg gtttgaacgc agcaaattga ggggctttct
    19141 tgccgtgatt gtcaggtgtc gacatggcat actccggtta agttttttct cattaaccga
    19201 aggatacact ctggagtcga ctccagagtc aagttttatc agaaatacag tgagcggacg
    19261 atgaggaagt gaccggcaaa gtagcaggcc gcagcaatcg cgttatccgc acggaagcgt
    19321 cggcgatagt ggctccccag ccagacaaag ttactgataa acagcaacga agcgccaaca
    19381 aacgcagaga gcgccggagc ggtcggacgg aagaaccaca gttcacctgc cagccacacc
    19441 atcaccagcg tcatgccgat aaacgtgcag ataggccaac gatactcttc cagccgcgtc
    19501 cagataatcg ccagtaacag cgcacccaga accagcaaca ccagtggcag aggccagaag
    19561 aaagagagcg tcatctgact ggcgaaatag atggtgtaca gcaggtgcga gaggaaaaac
    19621 gcgccgatgg cgtacatcag acgttgacgt ggcaacaggg ttagcgcatc gcccagcagt
    19681 gaggcgcaca gccctgccag caccagatag ctaatggcgt cgaacatcgg cgcttgccag
    19741 gccagtaaca gcaggagaag aagggttaac ggtttaaaca cccagcgttg ccaggttggc
    19801 ccacgatacg atgcatccac agatagccat gcggaaagac agacagcgat aaacgaccaa
    19861 agcatcttag ttccttgaat tcgttatttt ttcgtaagcg gaatgcctac ggtcttgctt
    19921 ccagtttagt ggcgagggtg gacggatgac aacaccataa ctagcagggt atgcttattt
    19981 ccgcattttc cgatgaggga taaagatgag caaaccacct cttttcttta ttgttatcat
    20041 tggcttaatt gtcgtcgccg catcgtttcg ttttatgcaa cagcgacggg aaaaagctga
    20101 taatgatatg gctccgctcc agcaaaagct ggtggtggtg agcaacaagc gggaaaaacc
    20161 gattaacgat cgccgttcgc gccagcagga agtgactccg gcaggcacca gtatgcgcta
    20221 tgaggcaagc ttcaaaccgc aaagcggagg aatggagcag acgtttcgcc tcgacgccca
    20281 gcagtaccat gccctgacag tgggcgataa aggtacgctg agctataaag gaacgcgctt
    20341 tgttagcttt gttggcgaac aataaggtta ttttttaact ttaaatttct tctgccagac
    20401 cagcagttca aacacgccga aaaggaaaat ccgcaccttt tcggcggtgg tcatctgcgg
    20461 gccatctttc ggtaaagtgg atttcaatag cgccagctgc ataccatgca tcagcacggt
    20521 aaaaatcagc gccacgttaa cgaaaatatt cagcgggcgc gggaagggat gcaccagatt
    20581 gaggattaaa aatccccaaa cgcagagcat taacaaacga ccaatattaa tcagcatcac
    20641 tttctccttg tgcttcgcgt tgatacagcc gataagccac ctgacccgcc actttttccc
    20701 gatgtaatga ccagtttgcc ggaacagtgg gcagaccgtt ttcgacttcg ctttcgacat
    20761 aaatcagggc ttcgtcagcc agccagccgt tatcttccag taaatttatc gtctcttcta
    20821 acaagccacg gcggaacggt ggatcgacaa acacgatatt atgcggtgta cctttttgcg
    20881 ccaggaatga catcgcgttg ctgttcacca cgcgtgcatt gcctgctttt agtgtcgcca
    20941 gattcttaat taactgctga gaaaccgcgc gatccatctc aatcaacgtg gcccccgcag
    21001 cgtagcgcga taacgcttcc agtcccagcg cgccgctccc ggcgaagcaa tccagacatt
    21061 gggcgtcaac aatgaccgga gccagccagt taaacaatgt ttcgcgtacg cggtcggtgg
    21121 tggggcgcag acctgggcta tcaggaaccg ggagtttacg gcctcgccac tgcccgccaa
    21181 taatgcggat ttggccgctg ccggaatgat tcggtttttt catgataaat tgctcaatcc
    21241 gccagataac acatacttgc aggcggtgat gtgaattaag ttaagtgata gactatttca
    21301 tcattttttt agctgctatg tacatagcgt taacgctgtg ccatgaagca acagcgagga
    21361 gtgtagtcgc aaatggcgaa agaaaaaaaa cgtggctttt tttcctggct gggctttggt
    21421 caaaaagagc agaccccgga aaaagagaca gaagttcaga atgaacaacc ggttgtagaa
    21481 gaaatcgttc aggcgcaaga gcctgcgaag gcttctgaac aagccgtcga agagcaaccg
    21541 caggcgcata ctgaagccga ggcggaaact tttgctgccg acgttgtgga agtcactgaa
    21601 caggttgctg aaagtgaaaa agcgcagcct gaagcggaag tcgttgcaca gccggaaccg
    21661 gtcgtagaag aaacgccgga gccagtggct atcgaacgtg aagagctgcc gttgccggaa
    21721 gacgtcaacg ccgaagaggt ttcgccagaa gagtggcagg ctgaagcgga aaccgtagag
    21781 attgtcgaag cggcggaaga agaagcggct aaagaagaaa tcaccgacga agagctggaa
    21841 gcacaggcgc tggctgccga agcggcagaa gaggcggtga tggtggttcc tccggtagaa
    21901 gagcagccgg tggaagaaat cgctcaggag caggaaaaac cgaccaaaga aggtttcttc
    21961 gcgcgcctga aacgcagcct gttaaaaacc aaagaaaatc tcggttccgg atttatcagc
    22021 ctgttccgcg gtaaaaaaat cgacgatgat ctgtttgagg agctggaaga acagctgttg
    22081 atcgccgatg tgggcgtgga aaccacacgt aaaattatca ccaatctgac ggaaggcgca
    22141 tcccgcaagc agcttcgtga tgccgaggcg ctctatggcc tgctgaaaga agagatgggc
    22201 gagattctgg cgaaagtcga tgagccgctg aatgttgaag gtaaaacgcc gttcgtgatc
    22261 ctgatggtgg gcgtcaacgg tgtgggtaaa actacgacga ttggtaagct ggcacgtcag
    22321 tttgagctgc agggtaaatc ggtgatgctg gcggcgggcg atactttccg tgcagcagcg
    22381 gttgaacagc ttcaggtctg gggtcagcgc aacaatattc cggtgattgc ccagcatacc
    22441 ggtgcggatt ccgcctctgt tatcttcgac gccattcagg cagctaaagc gcgtaatatc
    22501 gacgtcctga ttgccgatac cgccggacgc ctgcagaaca aatcgcacct gatggaagag
    22561 ttgaagaaaa tcgtccgcgt gatgaagaaa ctcgacgttg aagcgccgca tgaagttatg
    22621 ctgactattg atgccagtac cgggcagaat gcggtaagcc aggccaaact gttccacgaa
    22681 gccgttggct taaccggcat cacgctaacg aaactggacg gcacggcgaa aggcggggta
    22741 attttctcgg tggctgacca gtttggtatc cctatccgct acattggtgt cggcgaacgt
    22801 attgaggatt tgcgtccgtt taaggcggac gactttatag aggcactttt tgcccgagag
    22861 gattaacaat gattcgcttt gaacatgtca gcaaggctta tctcggtggg agacaggcgc
    22921 tgcagggcgt tacgttccat atgcagccgg gtgagatggc gtttctgacc ggtcattccg
    22981 gcgcagggaa aagtaccctc ctgaagctga tctgtgggat tgagcggccc agcgccggga
    23041 aaatctggtt tagcggccat gacatcacgc gtctgaaaaa ccgtgaagtt ccgtttctgc
    23101 gccgccagat tggcatgatt ttccaggatc accatctact gatggaccgt actgtctacg
    23161 ataacgtggc gatcccgctg attatcgctg gtgccagcgg tgacgatatt cgtcgccggg
    23221 tgtcggcggc actggataaa gtcgggctac tggacaaagc gaagaacttc cctattcagc
    23281 tttccggcgg tgaacaacag cgtgttggca ttgcccgcgc ggtggtgaat aagcccgcgg
    23341 tactgctggc ggacgaaccg accggtaacc tggacgacgc gttgtcggaa gggattctac
    23401 gtctgtttga agagtttaac cgcgttgggg taaccgtatt gatggcaacg cacgacatca
    23461 acctgatttc acggcgttcc tatcgcatgc tcaccctgag cgatggtcac ttgcatggag
    23521 gcgtgggcca tgaataagcg cgatgcaatc aatcatattc ggcagtttgg tgggcgtctg
    23581 gatcgcttcc gtaaatcggt cggcggctca ggcgacggcg gtcgtaacgc accgaaacgc
    23641 gcgaaatcct cgccaaaacc ggtaaatcgc aaaaccaacg ttttcaacga acaggtgcgc
    23701 tatgccttcc acggcgcatt gcaggatctg aaaagcaaac cgttcgccac gtttttaacg
    23761 gtgatggtta tcgccatctc cctgacgctg cccagcgtct gttatatggt gtacaaaaac
    23821 gttaatcagg cggcgacgca gtattatccg tcaccgcaaa tcactgttta cttgcaaaaa
    23881 acgctggacg atgacgccgc tgcgggcgtg gtggcacagt tgcaggccga gcaaggcgtg
    23941 gagaaagtga actatctttc tcgtgaagac gcactgggcg agttccgtaa ctggtctggt
    24001 tttggtggtg cgctggatat gctggaagaa aacccgcttc cggcagtggc ggtggtgatc
    24061 ccgaaactcg attttcaggg gacggaatca ctcaatacgc tgcgtgatcg tatcacgcag
    24121 attaacggca ttgacgaagt gcggatggat gacagctggt ttgcccgtct ggcggcgttg
    24181 accgggctgg tcgggcgcgt ttcggcgatg atcggcgtgt tgatggtggc ggcagtgttc
    24241 ctcgtcatcg gcaacagcgt acgtctgagc atctttgctc gccgtgactc catcaacgtg
    24301 cagaaactga ttggtgcgac ggatggtttc atccttcgcc cgttcctgta tggtggcgca
    24361 ctgctgggat tttctggcgc attgttgtca ttaattttgt cagaaattct ggtgctgcga
    24421 ttgtcatcgg cggttgcgga agtggcacag gttttcggaa cgaattttga tatcaatggt
    24481 ttatcgttcg atgaatgcct gctattgctg ctggtatgct cgatgattgg ctgggtggca
    24541 gcgtggcttg ccacggtaca acatttacgc cactttacgc ctgaataata aaagcgtgtt
    24601 atactctttc cctgcaatgg gttccgtagc agggaaagag atcccgttgt ctcttcccgg
    24661 tatttcatct ctatgtcaca ttttgtgcgt aatttattca caagcttgca ttgaacttgt
    24721 ggataaaatc acggtctgat aaaacagtga atgataacct cgttgctctt aagctctggc
    24781 acagttgttg ctaccactga agcgccagaa gatatcgatt gagaggattt gaatgactga
    24841 caaaatgcaa agtttagctt tagccccagt tggcaacctg gattcctaca tccgggcagc
    24901 taacgcgtgg ccgatgttgt cggctgacga ggagcgggcg ctggctgaaa agctgcatta
    24961 ccatggcgat ctggaagcag ctaaaacgct gatcctgtct cacctgcggt ttgttgttca
    25021 tattgctcgt aattatgcgg gctatggcct gccacaggcg gatttgattc aggaaggtaa
    25081 catcggcctg atgaaagcag tgcgccgttt caacccggaa gtgggtgtgc gcctggtctc
    25141 cttcgccgtt cactggatca aagcagagat ccacgaatac gttctgcgta actggcgtat
    25201 cgtcaaagtt gcgaccacca aagcgcagcg caaactgttc ttcaacctgc gtaaaactaa
    25261 gcagcgtcta ggctggttta accaggatga agtcgaaatg gtggcccgtg aactgggcgt
    25321 aaccagcaaa gacgttcgtg agatggaatc acgtatggcg gcacaggaca tgacctttga
    25381 tctgtcttcc gacgacgatt ccgacagcca gccgatggca ccggtgctct atctgcagga
    25441 taaatcatct aactttgccg acggcatcga agatgataac tgggaagagc aggcggcaaa
    25501 ccgtctgacc gacgcgatgc aaggtctgga cgaacgcagc caggacatca tccgcgcgcg
    25561 ctggctggac gaagacaaca agtccacgtt gcaggaactg gctgaccgtt acggtgtttc
    25621 cgctgaacgt gtacgccagc tggaaaagaa cgcgatgaaa aaactgcgcg ctgctataga
    25681 agcgtaattt ccgctattaa gcagagaacc ctggatgaga gtccggggtt tttgtttttt
    25741 gggcctctac aataatcaat tccccctccg gcaaaacgtc aatccccacg cagattgtta
    25801 ataaactgtc aaaatagcta ttccaatatc ataaaaatgg ggtatgtttt agcagagtat
    25861 gctgctaaag cacgggtagt catgcataaa acgaaataaa gtgctaaatc acaacaccac
    25921 aacacacgta ataaccagaa gaatggggat tctcaggatg aatataaagg gtaaagcgtt
    25981 actggcagga tgtatcgcgc tggcattcag caatatggct ctggcagaag atattaaagt
    26041 cgcggtcgtg ggcgcaatgt ccggtccggt tgcgcagtac ggtgaccagg agtttaccgg
    26101 cgcagagcag gcggttgcgg atatcaacgc taaaggcggc attaaaggca acaaactgca
    26161 aatcgtaaaa tatgacgatg cctgtgaccc gaaacaggcg gttgcggtgg cgaacaaagt
    26221 cgttaacgac ggcattaaat atgtgattgg tcacctctgt tcttcatcaa cgcagcctgc
    26281 gtctgatatc tacgaagacg aaggcatttt aatgatcacc ccagcggcaa ccgcgccgga
    26341 gctgaccgcc cgtggctatc agctgatcct gcgcaccacc ggcctggact ccgaccaggg
    26401 gccgacggcg gcgaaatata ttcttgagaa agtgaaaccg cagcgtattg ctatcgttca
    26461 cgacaaacag caatacggcg aaggtctggc gcgagcggtg caggacggcc tgaagaaagg
    26521 caatgctaac gtggtgttct ttgatggtat caccgccggg gaaaaagatt tctcaacgct
    26581 ggtggcgcgt ctgaaaaaag agaatatcga cttcgtttac tacggcggtt atcacccgga
    26641 aatggggcaa atcctgcgtc aggcacgcgc ggcagggctg aaaactcagt ttatggggcc
    26701 ggaaggtgtg gctaacgttt cgctgtctaa cattgcgggc gaatcagcgg aagggctgct
    26761 ggtgaccaag ccgaagaact acgatcaggt tccggcgaac aaacccattg ttgacgccat
    26821 caaagcgaaa aaacaggacc caagcggcgc attcgtttgg accacctacg ccgcgctgca
    26881 atctttgcag gcgggcctga atcagtctga cgatccggct gagatcgcca aatacctgaa
    26941 agcgaactcc gtggataccg tgatgggtcc gctgacgtgg gatgagaaag gtgatctgaa
    27001 aggctttgag ttcggcgtat ttgactggca cgccaacggc acggcgaccg atgcgaagta
    27061 atcattaatc ggcaactttg ggttgccgcc aaattgctaa tatcgagtac gttgcttcat
    27121 gccggatgcg gcgtaaacgc cttatccggc ctacaagatc caaagaaatc agtaaattgc
    27181 aacacacatt gtaggcctga taagcgtagc gcatcaggca atttctcgtt ggtcatcact
    27241 tttgaaatca acttgacgat taacgcttct cccagccgcc ctgttgcgcc gtaaatccca
    27301 gtgcctgcat aaacgccgtc atcacaccgc gatcttccac gcctgcatcc gccatccacc
    27361 agcatgaaac gccaggattg ttacgcaaaa cctcttccag cagatattgc cccacaccgc
    27421 gacgtcgggt gacttcccgc acacgcaggg aatccagtgc tccctcggtg ccgcttaagg
    27481 ttacccgtac ggcagcgagc aggcgctcgt taaaacgcgc ggcgtagata cggtggttat
    27541 cgtcaacctg taaagaggaa ggggaatact ccggccagat cttttgcagg tcaatccggt
    27601 cctggtcgct aaagttttct aatcgaatga tggtcagctt catgggtaac ccgtgtaaat
    27661 cacaaaagtg gaaccagtgt agcgaaataa tttaatcgga ggctttctct tttttatttc
    27721 ttttggcagg tgattaattt tttaacagca ataattacaa agttaaaaca ttagagaatg
    27781 aaaaataccc agcataatcc cctgaatggt agtgaattat tccgcccttt gtgccgttat
    27841 tttatgctga
//
LOCUS       DADLFA010000034        27676 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000034 DADLFA010000000
VERSION     DADLFA010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27676
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.034"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(211..420)
                     /gene="fumD"
                     /locus_tag="JQH65_001257"
     CDS             complement(211..420)
                     /gene="fumD"
                     /locus_tag="JQH65_001257"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HAY6138736.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELDKQAMETVINALVK"
     gene            977..2389
                     /locus_tag="JQH65_001258"
     CDS             977..2389
                     /locus_tag="JQH65_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase I"
                     /protein_id="HAY6138737.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            2700..2936
                     /locus_tag="JQH65_001259"
     CDS             2700..2936
                     /locus_tag="JQH65_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006675846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major outer membrane lipoprotein"
                     /protein_id="HAY6138738.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(3000..4004)
                     /gene="ldtE"
                     /locus_tag="JQH65_001260"
     CDS             complement(3000..4004)
                     /gene="ldtE"
                     /locus_tag="JQH65_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HAY6138739.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLLPVVPAGPNNPLGRYALRFAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRARQ
                     "
     gene            complement(4153..4569)
                     /gene="sufE"
                     /locus_tag="JQH65_001261"
     CDS             complement(4153..4569)
                     /gene="sufE"
                     /locus_tag="JQH65_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HAY6138740.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQNQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            complement(4582..5802)
                     /gene="sufS"
                     /locus_tag="JQH65_001262"
     CDS             complement(4582..5802)
                     /gene="sufS"
                     /locus_tag="JQH65_001262"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HAY6138741.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLHTL
                     FDEKTRLLAITHVSNVLGTENPLAGMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSVLGLNNIAEYELNLMHYALSQLESVPNLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            complement(5799..7070)
                     /gene="sufD"
                     /locus_tag="JQH65_001263"
     CDS             complement(5799..7070)
                     /gene="sufD"
                     /locus_tag="JQH65_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAY6138742.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTSLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMYITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            complement(7045..7791)
                     /gene="sufC"
                     /locus_tag="JQH65_001264"
     CDS             complement(7045..7791)
                     /gene="sufC"
                     /locus_tag="JQH65_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAY6138743.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLAVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            complement(7801..9288)
                     /gene="sufB"
                     /locus_tag="JQH65_001265"
     CDS             complement(7801..9288)
                     /gene="sufB"
                     /locus_tag="JQH65_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAY6138744.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            complement(9297..9665)
                     /gene="sufA"
                     /locus_tag="JQH65_001266"
     CDS             complement(9297..9665)
                     /gene="sufA"
                     /locus_tag="JQH65_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HAY6138745.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            complement(10213..10401)
                     /locus_tag="JQH65_001267"
     CDS             complement(10213..10401)
                     /locus_tag="JQH65_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6138746.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            complement(10501..10911)
                     /gene="menI"
                     /locus_tag="JQH65_001268"
     CDS             complement(10501..10911)
                     /gene="menI"
                     /locus_tag="JQH65_001268"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HAY6138747.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            complement(10908..13964)
                     /locus_tag="JQH65_001269"
     CDS             complement(10908..13964)
                     /locus_tag="JQH65_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAY6138748.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDVLDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDQEMLGLNIVEFAGDDEALIDERVNALCARLDELIASHQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPELDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTL
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            14353..15465
                     /gene="ydiK"
                     /locus_tag="JQH65_001270"
     CDS             14353..15465
                     /gene="ydiK"
                     /locus_tag="JQH65_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HAY6138749.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            15651..15758
                     /gene="rprA"
                     /locus_tag="JQH65_001271"
     ncRNA           15651..15758
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="JQH65_001271"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            15894..16273
                     /locus_tag="JQH65_001272"
                     /pseudo
     CDS             15894..16273
                     /locus_tag="JQH65_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014786.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
     gene            16218..16940
                     /locus_tag="JQH65_001273"
                     /pseudo
     CDS             16218..16940
                     /locus_tag="JQH65_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707590.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            16952..17818
                     /gene="ydiB"
                     /locus_tag="JQH65_001274"
                     /pseudo
     CDS             16952..17818
                     /gene="ydiB"
                     /locus_tag="JQH65_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
     gene            17850..18608
                     /gene="aroD"
                     /locus_tag="JQH65_001275"
     CDS             17850..18608
                     /gene="aroD"
                     /locus_tag="JQH65_001275"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAY6138750.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVACAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            18754..20349
                     /locus_tag="JQH65_001276"
     CDS             18754..20349
                     /locus_tag="JQH65_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HAY6138751.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQSQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGLAFGANVNTRAILDMTSQFNFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEIADGKLNIVQEGRVKKFIQELPEI
                     TFSGKIVLERELDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            20363..21514
                     /locus_tag="JQH65_001277"
     CDS             20363..21514
                     /locus_tag="JQH65_001277"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HAY6138752.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFAEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(21557..22468)
                     /locus_tag="JQH65_001278"
     CDS             complement(21557..22468)
                     /locus_tag="JQH65_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY6138753.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLSNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSEFPQPVSLVG"
     gene            22782..23553
                     /locus_tag="JQH65_001279"
                     /pseudo
     CDS             22782..23553
                     /locus_tag="JQH65_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
     gene            23573..24511
                     /locus_tag="JQH65_001280"
     CDS             23573..24511
                     /locus_tag="JQH65_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HAY6138754.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAAPLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            24567..25856
                     /locus_tag="JQH65_001281"
     CDS             24567..25856
                     /locus_tag="JQH65_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY6138755.1"
                     /translation="MSDDKFDAIVVGADVASSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILQANVVVLAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQMVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KEREDFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYSRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            25853..26146
                     /locus_tag="JQH65_001282"
     CDS             25853..26146
                     /locus_tag="JQH65_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HAY6138756.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            26149..>27676
                     /gene="fadK"
                     /locus_tag="JQH65_001283"
     CDS             26149..>27676
                     /gene="fadK"
                     /locus_tag="JQH65_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416216.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HAY6138757.1"
                     /translation="MHPTGPHLGPDVLFRESKMKVTLTFNEQRRAAYRQQGLWGDASL
                     ADYWQQTARAMPDKIAVVDNHGATYTYSALDHAASCLANWMLAKGIESGDRIAFQLPG
                     WCEFTVIYLACLKIGAVSVPLLPSWREAELVSVLNKCQAKMFFAPTLFKQTRPVDLIL
                     PLQNQLPQLQQIVGVDKLAPATSSLSLSPIIADNTSLTTAITTHGDELAAVLFTSGTE
                     GLPKGVMLTHNNILASERAYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARS
                     VLLDIFTPDACLALLEQQRCTCMLGATPFVYDLLNVLEKQPADLSALRFFLCGGTTIP
                     KKVARECQQRGIKLLSVYGSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDAR
                     KTLPPGCEGEEASRGPNVFMGYFDEPELTARALDEEGWYCSGDLCRMDEAGYIKITGR
                     KKDIIVRGGENISSREVEDILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLS
                     L"
BASE COUNT         6905 a         7001 c         6803 g         6967 t
ORIGIN      
        1 aactgatatt tattatcatt tgaaataaat ttaacttaag cgacaattat ttgtgacgtt
       61 cattgcagaa aataaaaata atattatcgt tataaattaa aatacatacc gcaattcgta
      121 taatttcagc agttgttatt tagaatgatt aactggaccg caactgaagc aagaatatca
      181 ggatgaagct taccgcctca tcctgaatgg tcattttacc agtgcgttaa tgacggtttc
      241 catcgcctgc ttatccagct cactgcgctg aatgcgttta ttggctaatg ctgtacgtaa
      301 tacgcctgcg ataacaatat gctttggttc ctgccccaga tcgcgcattt ccagcacaac
      361 tttaccgaca actctgcaca tttctcggta cagttcatcc tcttttgtcc gatttcccat
      421 tctttatacc tatttatcat gccaactatc agcatatatc aatctaacca attaaacaaa
      481 aatcaaacaa aatcagacaa ataacgcgat aaattatttt aactgtagca attgagcgat
      541 gatatattta tacaccggat gaactttcac ttatcctcac actgacaact ttggcaccag
      601 acgttgcgca aacagcgaag tttttgcgta accttttccc tggaacgtta aatctttgat
      661 aacaatttat tgtctaacaa gttgtatatt ttttgaaacg ctgtttttgt tttccttttg
      721 gattaatttc agcgtataat gcgcgccaat tgactcttga atggtttcag cactttggac
      781 tgtagaactc aacgactcaa aaacaggcac tcacgttggg ctgagacaca agcacacatt
      841 cctctgcacg ctttttcgat gtcacttatc cttagagcga ggcaccacca ctttcgtaat
      901 accggattcg ctttccggca gtgcgcccag aaagcaagtt tctcccatcc ttctcaactt
      961 aaagactaag actgtcatga aaaagaccaa aattgtttgc accatcggac cgaaaaccga
     1021 atctgaagag atgttagcta aaatgctgga cgctggcatg aacgttatgc gtctgaactt
     1081 ctctcatggt gactatgcag aacacggtca gcgcattcag aatctgcgca acgtgatgag
     1141 caaaactggt aaaaccgccg ctatcctgct tgataccaaa ggtccggaaa tccgcaccat
     1201 gaaactggaa ggcggtaacg acgtttctct gaaagctggt cagaccttta ctttcaccac
     1261 tgataaatct gttatcggca acagcgaaat ggttgcggta acgtatgaag gtttcactac
     1321 tgacctgtca gttggcaaca ccgtactggt tgacgatggt ctgatcggta tggaagttac
     1381 cgccattgaa ggtaacaaag ttatctgtaa agtgctgaac aacggtgacc tgggcgaaaa
     1441 caaaggtgtg aacctgcctg gcgtttccat tgctctgcca gcactggctg aaaaagacaa
     1501 acaggacctg atctttggtt gcgaacaagg cgtagacttt gttgctgctt cctttattcg
     1561 taagcgttct gacgttatcg aaatccgtga gcacctgaaa gcgcacggcg gcgaaaacat
     1621 ccacatcatc tccaaaatcg aaaaccagga aggcctcaac aacttcgacg aaatcctcga
     1681 agcctctgac ggcatcatgg ttgcgcgtgg cgacctgggt gtagaaatcc cggtagaaga
     1741 agttatcttc gcccagaaga tgatgatcga aaaatgtatc cgtgcacgta aagtcgttat
     1801 cactgcgacc cagatgctgg attccatgat caaaaaccca cgcccgactc gcgcagaagc
     1861 cggtgacgtt gcaaacgcca tcctcgacgg tactgatgca gtgatgctgt ctggtgaatc
     1921 cgcaaaaggt aaatacccgc tggaagcggt ttctatcatg gcgaccatct gcgaacgtac
     1981 cgaccgcgtg atgaacagcc gtctcgagtt caacaatgac aaccgtaaac tgcgcattac
     2041 cgaagcggta tgccgtggtg ccgttgaaac tgctgaaaaa ctggatgctc cgctgatcgt
     2101 ggttgctact cagggcggta aatctgctcg cgcagtacgt aaatacttcc cggatgccac
     2161 catcctggca ctgaccacca acgaaaaaac ggctcatcag ttggtactga gcaaaggcgt
     2221 tgtgccgcag ctggttaaag agatcacttc tactgatgat ttctaccgtc tgggtaaaga
     2281 actggctctg cagagcggtc tggcacacaa aggtgacgtt gtagttatgg tttctggtgc
     2341 actggtaccg agcggcacta ctaacaccgc atctgttcac gtcctgtaat attgcttttg
     2401 tgaattaatt tgtatatcga agcgccctga tgggcgcttt ttttatttaa tcgataacca
     2461 gaagcaataa aaaatcaaat cggatttcac tatataatct cactttatct aagatgaatc
     2521 cgatggaagc atcctgtttt ctctcaattt ttttatctaa aacccagcgt tcgatgcttc
     2581 tttgagcgaa cgatcaaaaa taagtgcctt cccatcaaaa aaatattctc aacataaaaa
     2641 actttgtgta atacttgtaa cgctacatgg agattaactc aatctagagg gtattaataa
     2701 tgaaagctac taaactggta ctgggcgcgg taatcctggg ttctactctg ctggcaggtt
     2761 gctccagcaa cgctaaaatc gatcagctgt cttctgacgt tcagactctg aacgctaaag
     2821 ttgaccagct gagcaacgac gtgaacgcaa tgcgttccga cgttcaggct gctaaagatg
     2881 acgcagctcg tgctaaccag cgtctggaca acatggctac taaataccgc aagtaatagt
     2941 acctgtgaag tgaaaaatgg cgcacattgt gcgccatttt ttttgtctgc tatttaccac
     3001 tactgcctcg cgcgtaacat attcccttgc tctggttcac cattctgcgc tgactctact
     3061 gaaggcgcat tgctggctgc gggagttgct ccactgctca ccgaaaccgg ataccctgcc
     3121 cgacgataca acgctttatc gactaacttc tgatctacag ccttattgtc tttaaattgc
     3181 gtaaagcctg ctggcagtgt gtatggcatt gtctgaacgt tctgctgttc ttctgccgat
     3241 agtggtcgat gtacttcaac ataacgcatc ccgttaggct ccacggaata tttcaccggt
     3301 tcgttgatca ctttcaccgg cgttcccgtc cgcacgctgg agaataaggc tttaatatcc
     3361 ggtgcattca tgcgaataca gcccgaactg acgcgtaaac cgacgctgtc cggcgcactg
     3421 gtaccatgaa tgaggtattc gccattacca tgcgcgaagc gcagtgcgta acgtcctagc
     3481 gggttatttg gtccggcagg aacgactggc agtaatttaa tgccacgctc cagcgaacgt
     3541 tgacgaatgc ctgccgtagg cgtccaggtt ggattaggga ttttctgccc cacacgcgtt
     3601 tccatcaccg gcgtttccag cccctgcaag ccaataccta tcggataaac ctgcacgata
     3661 ttttctcccg gcggataata ataaaggcgc agctctgcaa ggttaacgat aatcccctga
     3721 cgcggtgcat caggtaataa cagttgtgaa ggaatagtta tcgtcgtacc aggttttggc
     3781 accggggcga tagtgttatt ggcttcaagg atcaacattg ccgcagtatc aaaacgtcgg
     3841 gcaatagcct gaaggttttt atccccttct tgcaccgtat acgtttgatt ttgcccaacc
     3901 agtcggcttc cggttggtgg tagcggataa tcaaccgccc aggcagcctg gatggcgcta
     3961 aaagcgccga taagcgtgag tgtaagcaaa gacgcgcgtt tcattgtaaa cctcctgtat
     4021 ttgccggaga ctcacgctga aacgtcggat ggcgcttatg ttcacctgaa accaaaacac
     4081 tcctgtgcag gtcagtgtaa acattgacca tccggcaatg tgagccaacc ggatgaaagc
     4141 tgtcccttta gtttagctaa gtgcagcggc tttggcgcga attgcgcgaa tcatcgcttc
     4201 cagaccttgt gaacgagatg gggtgagatg ttgggtgagc gccatttttt caaaccacgg
     4261 acgcacatcg aaattgacaa tatcctgcgg cgtcatctga tcgtagagaa taaatacgac
     4321 cgcaataagc cctttcacaa tcgccgcatc gctgtcgccc tgcaattcaa taattccctg
     4381 cgcattctgg cgcatgacaa tccacacctg attctggcag ccctgaatgc tattttgtgg
     4441 gcttcggtct tcgtcgcgta attctggcag acgctggccc agctcaataa tgtagagata
     4501 tttctcttcc cagttggcgc agcgtaaaaa attacgcagc aatttttctt tatccggcaa
     4561 taaagccata gtgcctccct gttatcccag cagacggtga atacgttgca ggccggtcac
     4621 cagacgatcc acttcttcat gagtgttata catggccagc gacgcccgac acatcgcagg
     4681 gacgttgtaa taggccatta atggcatagc gcagtgatgt ccggtacgca cagcaatgcc
     4741 gtaattatcg agaaaactgc caacatcata ggcgtggtgt ttaccgagat taaaagcaat
     4801 aacgccaagc ctgttttgtg ggccatagag agtgagattc ggtacagatt ccagctgtga
     4861 tagcgcgtaa tgcatcagat tcagttcata ctcggctatg ttattaagcc ccagcaccga
     4921 aacatattcc agcgccgcgc caagaccaat gatgcccccg gtattgggtg taccggcttc
     4981 aaaccgccat ggtgctttgg tccaggtagt gccttcactc aggctgacgg tggcgatcat
     5041 agaaccgccc ccttcccacg gcggcatctc ctgcaacaag gcttctttga cataaagaat
     5101 gccaatcccg gtggggccat acagtttatg cccggaaaac acgtaaaaat cgcaatccag
     5161 cgcctgaaca tccaccggat gatgcatcac cgcctgagcg ccatccacca gcacttttgc
     5221 gccatgctgg tgcgcaagcg tgatcattcc cgccagtgga ttttctgtgc caagcacgtt
     5281 ggagacatga gtaattgcca gcaggcgagt tttctcatca aacagcgtat gcagcgtctc
     5341 cagttgcagc gtaccgtcgg ggttgagagg gatcacacgc agctctgcgc caacgcgtgc
     5401 gcaaagcatc tgccagggga caatgttagc gtggtgctcc atctgactga tgatgatgtt
     5461 atcgcccgcc cgcacattgc tgttgcccca gctattggcg accagattga tcccttccgt
     5521 cgtgccgcgg acgaacacca gctcttccgc cgaacgggca ttaataaaca gcgatgcccg
     5581 cttgcgcacg ttctccattt tctcggtcgc ctgggcgctt aaggtatgaa taccgcgatg
     5641 caccgccgcg tagccatgac gataaaactc ggcctcggcg tcaatcacct ggctcggttt
     5701 ctgtgcactg gcggcgctgt cgagataagc cagcggcaaa ccgtttacct cacgcgaaag
     5761 caccggaaag tcggcccgca ctttgtcgac ggaaaaagtc atcttgcacc tcctggcagc
     5821 cgttgaccga ttcgggccag cacctgctgt ttaagcccct catcacgcag tgcttccgtc
     5881 agttcggcag caaaggcgta aatgatcatc tgctgggcat cctgctgatt gatcccgcgc
     5941 gagcgcagat agaacatctg ttcatcatca atacgcccca ccgtcgcgcc gtggctgcat
     6001 ttcacatcat ctgcatagat ttccagctgc ggtttcgtat ccacttccgc cagtttgccc
     6061 atcagcagat tattgttggt catctgacca tccgttttga tggcgtgctg cgcgacgttg
     6121 atcaaaccgt taaataccgc gcggcctttg tcgctgacga tagttttgtg caactgtcgg
     6181 ctgttacaaa aacctttatt gtgttccagc caggtacggg tatcacacac ctcgtttttc
     6241 accggcatcg ccaggctatt gatccgcagc gtgctgtttt cgccattgag ttgcgtactg
     6301 gtgttgtgtc gtaacactgc gccacccagc aggaaactat ggctaaatgc ggtggcatcc
     6361 tcagccagca acaaatcgtt atgagcaaag tggtgactga gcgggttttc aaacgccagc
     6421 ttgatatgct gcaagtgggc gttcgctgcg acattgatag tgaaccgtgc cccggtaaaa
     6481 tgacgagcat cattaaggct gacaaaatgt tcgatcaccg ttgcttcggc accttccgcc
     6541 agatccagat gatgccggta atgggcagtg ttcacctctt cacctgccac gccctgggtg
     6601 atatacatta acagcaatgg ctttgccggc cgttgaccgc gcttcacggc gatatgcgtc
     6661 acgctttgtg ccaggctttc cgtcaaatgc agaaacactt ccgcctgaat agcgtcgggt
     6721 aacccctgac ggtcgtcgtt aatgctcact tcatatccgc tgccttcaat tgcatcgctc
     6781 agcgcgggca cgtaacgtcc atcgacaaac accagccgca cggcgtctaa cgttaacgct
     6841 aaggcatcac gctgctgtgg ggatatctcc cccgcaatgc tgacaaactg gctattggtc
     6901 agcccttcca gcgacgtata tttccagttt tcatgtttac gtgtcggcag tccggtacgc
     6961 agcaattgtt gtaaatgctg ctgtgcttgc ggggagcgtt ttgtcccttc agcttcaaac
     7021 aagtgatgcc actgttgcag cgcgttactg ctgttcggta agccagccat aaccctgctc
     7081 ctccagttgt ttgaccaacg tgaaatcgcc ggatttcaca attcgtccct gatatagcac
     7141 atggacgtaa tcaggcttga tgtagtcgag aatgcgttgg tagtgcgtaa caatgatgaa
     7201 tgagcgcttg ccatcacgca gggagttcac gccatcggcg accactttta atgcgtcaat
     7261 atccagccca gagtccgact catcaagaat gcataactcc ggttccagca ccgccatttg
     7321 caaaatatcg ttgcgctttt tctcgccgcc ggaaaaacca acgtttaccg aacgggttaa
     7381 taaatcttcc ggcatcttca ggagagcgat tttctcttcc atcaaatcct gaaaatcaaa
     7441 gcggtcgagc gtttcctggc cgcgatagct gcgcactgca ttaagtgccg tttgcaggaa
     7501 aaactggtta ctgacgcctg gaatctccac cggatactgg aaggccataa agatgccttc
     7561 gcctgcacga tcttccggcg acagtgcaag caaatctttg cctttgaact caaccgtgcc
     7621 gcccgtcact tcataatctt cgcgcccggc aagcgttgcc gataaggtac ttttgcccga
     7681 accgtttggc cccataatgg cgtgaacttc gccgggacga acggcgaggc ttaatccacg
     7741 caggatagct ttatcttcca cgctgacgtg taaatcttta atacttaaca tgtttattcc
     7801 ttatccgacg ctgtgttcaa gactgatcgc gaggagtttt tgtgcttcaa cggcaaattc
     7861 cagcggcagt tctgagaaca cgtctttgca gaaaccgtta acaatcatcg agatggcgtc
     7921 ttcttcgctg atcccgcgtt gcaggcagta aaacagttga tcttcaccaa tacgtgatgt
     7981 cgttgcctcg tgctccagtt gtgcgctgtt attgcgacat tcgacatacg ggaaggtatg
     8041 cgccccacaa ttagcgccaa tcagcattga gtcgcactga gtgaaattgc gcgcattggt
     8101 tgccgtcggc atgattttca ctaagccgcg ataactgttc tgactatgtc cggcagagat
     8161 ccctttcgag ataatggtcg atttggtgtt tttaccgatg tggatcatct tggtgccggt
     8221 atccgcttgc tgatgaccgc tggtcagcgc cactgagtaa aactcaccaa tggagttatc
     8281 gccgcgcaaa atgcagctgg gatatttcca cgtaatcgcg gacccggttt ctgattgcgt
     8341 ccatgacatt ttgctgtttt cgccttcgca caaagcacgc ttggtgacga agttgagaat
     8401 accgccggtg ttgttatcgc cagggaacca gttttgtacc gtggaatatt tcacctcggc
     8461 gtttttatgg atgatgactt ccaccactgc cgcgtgtaac tgatagctgt cacgcaccgg
     8521 agcggaacag ccttcaatgt agctgacgta gctgtcttcg tcggccacca gaatggtgcg
     8581 ctcaaactgc ccggtttttt ccgcgttaat gcgaaaatag gtggaaagtt ccatcgggca
     8641 gcgcacgcct ttaggcacat aaataaacgt accgtcagag gctaccgccg cattaagcgc
     8701 ggcaaagaag ttgtcattcc ccggcaccac ggtgccgaga tatttacgca ccagttccgg
     8761 gtgatcgtgg atcgcctcac caaaggaaca gaaaataatt ccctgctccg ccagtttttc
     8821 gcgataagta gtggcaaccg aaacagagtc gaaaatggca tccaccgcca cctctttgcc
     8881 ttcccgcacg ggaacgccca actgctcaaa cgccgcctcc acctctttac ttaaaaaggc
     8941 gttcgcgcca gtttgctgca ccgcgccagg ttcagacgcg caagtgtcgt cacaattacc
     9001 gcacgatggt gctgagtagt agctgtaatc ctgataattc agcttgtcgt agtgcgcttt
     9061 caaccagtgc ggttcttcca tctccagcca tgcgcgatag gcgtttagac gaaactccag
     9121 catccactcc ggctcattac gcttcgccga aattgcgcgc accacctctt cgtttatccc
     9181 ctttgccagc tcatcggtgg ctaactgggt gaagaatcct tctttataat tcagcgggcc
     9241 gccggtccag gttttgacat cgtcagttgc ttcagtatta cgagacatag taccgcctat
     9301 accccaaagc tttcgccaca gccacattca ttctgggctt tagggttgtg aaatttgaat
     9361 atctgattaa gtccttcacg aacgaaatcg acttccgtgc catcaataaa cggcatcgct
     9421 tgcagcggga caaacagctt cgcgccgtcg tgttcaaaca gcagatcgtc tttgtccggc
     9481 tcgctaacac tgtcgagcac atagccaaag cccgcgcagc ccgtttgctt cacgcctaaa
     9541 cgcacgccga ccatacccgg ctgctttgcc accagctcac ggatgtgtat cgccgctgcg
     9601 ggtgtcagcg ttaagccttg ccaggcgaaa tcttgtgggt taaaggttcc tgaatgcatg
     9661 tccatcgatt tacctcactt catcgctttc agcgtataac agcatgttgg tgataatgat
     9721 tatcagttca acccagcaaa cgcaggggct ttaccgtaaa acatgctttt tgcctgcttt
     9781 taataagcat agaccctgat gtgtgggtta acaggcacgc taagaattag cgtatctcat
     9841 tgttagataa tggttattat ctaaggagca ttaaggctgt gatgaaaaag aaaaagttgt
     9901 attaaaatga ctatttaaga gataggtaaa aaagtgcagc gttcagaaat aagaaaaccc
     9961 ttaagtctgt gcgacacagg cttaagggtt tctaccccat ccggcgctta tctccggcac
    10021 tctcagtggc ttagctcttg aaggggcgat aagaataatc tcataaagct aacccgccgt
    10081 tttaacacaa actgcgatta gtattatttt tgaacaatat caggcggtag ataagcagta
    10141 ttaagaaggt catcgaacct ggacggaggt taatccaggt cgatttggcg aacttgcggc
    10201 attaagtcag gatcaatgca cgcccagacg ccaggcaaag tagatttctt cttttaattc
    10261 agcagaagag agagtaagca ggtcagcaaa ttcaagttct agttgtttca gacgtttgag
    10321 atattgggca ggtgaaagat tgctctggtc acggcgtaaa aattcaatgg ccagctgggt
    10381 gggatcaagt tgagtagaca tagcatcctc gcttttagac aagacgtgta cagtatacca
    10441 ccgtttactg tgcagataat gaccaaaagc aatatgcgtc acacttttct ggtgacaacg
    10501 tcacaaaatg gcggtcgtca atcgtgacga acagcacaaa cgccctttct catcgaagat
    10561 ttcaatctgc cagacctggt gacgcgaacc gagatgcaac ggtttacata tgccgcgcac
    10621 ccgcccttct cgtgccgagc ggacgtgatt agcattgatt tccagaccaa ccactttttg
    10681 ctcaccttcg gtacataaat aaccggcaac ggaaccgata ctttcggcca gtaccacgga
    10741 tgctcctcca tgcagcaacc cgaaaggctg ctttgtccgc gagtctactg gcattgtcgc
    10801 ttcaagggtg tcatcaccaa tatgttcaaa gcgaatatcc aggaacccca ccatgtttcc
    10861 ttcacccata gcattcagtg cttccagggt gattttccgt ttccatatca tttaataatc
    10921 tccagtaaag cctgcacagg atggcgtacc cccgtgcctt caacccgttt tacctggcta
    10981 cggcaggaat atccggtcgc cagacagcgg ttacgcggca gtcgctgcat agcctgatgc
    11041 caggataact catagatccc gagcgaattt tcatggtttt tcgcttcatg tccgtaagtc
    11101 cctgccatgc cgcagcaacc cacgctgaca ttttccagtt tcgcgccaaa acgggcaaat
    11161 atcgcggccc attgtgctgg cgcacccggc aaggcggtaa cttcggtaca gtgaccaaag
    11221 aaataccatg attcaccgct gactgtagcc acaggctgtg actcaagtgc gcttgccagc
    11281 cattcattcg ccagtaagac gttaaactcg ccacgctcct cgcccagcgc cagtttatat
    11341 tcatcgcgat aacaaagtac cagcgccgga tcgacgccca ccattggcat acccagcttc
    11401 gccatacggt tgaggaaatc cgccgtcttt ttcgccgtct tcgcaaaacg attaagaaaa
    11461 cctttaatat gctgggcttt gccatttggc gaaaatggca gtaacacagg ctggaaacct
    11521 aatttttcga ccagacggac aaaatccgcc accacttgcg catcgtaata gctggtaaag
    11581 gggtcctgca ccaccaacac tgtgcgcgct ttctgctctg cattgagaga ttcaagctgt
    11641 tccagcgtca tgtttgccga gcgatgcccc accatttgtt gttgtagcga ggggaccgac
    11701 agcagcggca aatcaaccat gccgatatgt ttttccgaga gtttgcgcac cagcggctgg
    11761 ttaatgaaga agttaaaggt tttcggcgcg cgtgccatca gcggcgcgta gctctcgacc
    11821 gtagcgacga ggtggtcgcg cagcgggcgt aaataacggg tgtgatagag ctgcagaaaa
    11881 cgagagcgaa actccggcac atcaattttg atggggcaca gggtcgaaca cgctttacag
    11941 gccagacagc ccgacatcgc ctctttgact tcgtgtgaga agtcatattc gcctttattc
    12001 gcatgccagc tattgcgcgt gcgggcaatt aacgtccgca aactgacgcc ggattcaggc
    12061 agttcttgtt ccagtttgag tggatcaacg ccgcgatccg ccaacaaacg cagccattca
    12121 cgcaccagcg ttgcgcgccc tttcggtgaa tgaatccggt tctgggtgat cttcatcgac
    12181 ggacacatcg gactacgggc atcaaagttg aagcataaac cgttgccgtt acactccatc
    12241 gcaccgcgcc actgctggcg taccgcaatg gggatctgcc gatcgaatgt accgcgcttc
    12301 accgcgtcca ctttcatcat cggcgcatcg agaccttctg gcgggcaaat cttccctggg
    12361 ttgagtcggt tatgcgggtc aaatgccgct ttcactttgc gcagttctgc aaaaagttcc
    12421 tcaccgaaaa acgccgggct gtattcagcg cgaaaacctt tgccgtgctc gccccacaac
    12481 aaaccaccgt atttcgcagt cagcgccacc acgtcatcag agatttgctt catcaaaatc
    12541 tcttgttgag gatcgcacat atccagctct ggacggacgt gcaagacacc tgcgtcgacg
    12601 tgaccgaaca taccgtagct taagccgtgg ctgtcgagca gcgcgcgaaa ttcagcaata
    12661 taatccgcca ggtgttccgg cggtacgcag gtatcctcag caaacggaat tggcttagcg
    12721 gcacctttgg cattgccaag cagaccaacg gcttttttgc gcattgcata gatacgttca
    12781 acccccgcca gctcacggca cacctgccag ccgatgacac ctgcttgatg actggcgatc
    12841 agctcatcca accgcgcaca gagtgcattt acccgctcat caatcagcgc ctcatcatca
    12901 ccagcaaatt ccacaatgtt cagcccgagc atctcttggt caggcacatc ggtaatcaac
    12961 tcgctgacgg aatgccagac aatatcttcc cgcgccagat tcagcacttt tgagtccacc
    13021 gtctctaccg aaagcgcccg cgcctcaacc ataaacggcg cgttacgcag cgcagagtca
    13081 aaagagtcat atttgacgtt caccagacgg cgcactttag gcaagcgcgt aatatccagc
    13141 cgcgcttcgg taataaaggc cagcgtccct tctgaacccg tcagaatgcg cgtcaggtcg
    13201 aactcggtca tctcatcgtt aaagacatga cgcagatcgt aaccggtaag aaagcggtta
    13261 agtttgggga agttgtcgat aattaactgg cgttgctgac ggcaacgttg ataaaccgtg
    13321 ttataaattc gcccgattgt ggtattggat ttacccagcg tttccgccaa ttcgacgggt
    13381 aaaggttgcg tatcgagaac atcgcccccc aacaacaccg cgcgtacgcc aagtacgtga
    13441 tctgacgttt tgccatagac cagcgatccc tgaccggatg catcggtatt gatcatccca
    13501 ccgagcgttg cccggttgct ggtcgaaagt tccggcgcaa aaaagtagcc gaacggtttc
    13561 aggtactgat tgagttgatc ttttatcacc ccggcctcaa cgcgcaccca gccctcttca
    13621 gggttaattt cgatgatgcg gttcatatgg cgggacatat caacaataat cccctggttg
    13681 agcgcctgac cgttagtgcc ggtgccgccg ccgcgggggg taaagatcag cgatgaatag
    13741 cgttcctgcg cggcaagacg ggcgatcagc gccacatctg cggttgaacg cggaaatacc
    13801 accgcatcgg ggagaagttg gtaaatactg ttgtcggtcg acattgtcag acgatcggca
    13861 taacttgtcg ccgtatcgcc ggtaaaacct tgttgctcca gctcttgcaa aaaattaagc
    13921 accagttgaa cgacgccggg tgcctgagaa atctgtggaa tcattatatt gcccctttcc
    13981 tgcggtctgt gatgtaggtc gatacactat tctttcaggc ggctgcaata gcgcactgaa
    14041 aggtgatgtt tatttactct atggatttcg agttgcagga aggcggcaag cgagtgaact
    14101 ccaggagctt acaatagtaa gtgactgggg tgaacgaacg tagccgcagc acatgcaact
    14161 tgaaatacga cgagtaaatc gtttgcgtgt tgcctgagtt gttgtaccac atttttttct
    14221 aacacgccca tcagaattaa gggcagaatc ggcctgttaa aaaccgctga aattgctcat
    14281 cattatgcag gtgagtttcg cgtgttcacg tcgcgtcgac gatttgacgc acaaaaaagg
    14341 tgaaaagtag ttatggtaaa tgttcgtcag cccagggatg tcgcacaaat tctgctttcg
    14401 gtgctgtttt tagccatcat gattgtggca tgtctgtgga ttgttcaacc ctttattctc
    14461 ggctttgcat gggccggtac ggtggttatc gccacctggc cggtattgtt acgtttgcaa
    14521 aagatcatgt ttggccgccg ctctctcgcc gttctggtga tgacgctgtt attagtgatg
    14581 gtgtttatca tccctattgc tttgctggtt aacagtatcg tcgacggcag cggcccgcta
    14641 attaaagcca tttccagcgg cgacatgacg ttacccgatc tggcgtggct taataccatt
    14701 ccggtgattg gcgcgaagct gtatgcaggc tggcacaact tgctggatat gggggggacg
    14761 gcgatcatgg cgaaagtccg cccttatatt ggcaccacca ccacctggtt cgttgggcag
    14821 gcggcgcata tcgggcgctt tatggtgcat tgtgcgctga tgcttctctt cagtgccctg
    14881 ctgtactggc gcggtgaaca ggtggcacaa ggcattcgcc attttgcaac ccgtctggca
    14941 ggcgttcgcg gagatgccgc cgtactgctg gcggcacagg ctatccgcgc ggtggcgctg
    15001 ggtgtggtgg tgacggcgtt agtacaggca gtgcttggcg gtatcggcct cgccgtatcc
    15061 ggcgtacctt atgcaacgtt gctaacggtg ttaatgatcc tctcctgcct tgtccagctt
    15121 ggcccgttgc cggtactgat tccggcgatt atctggctct actggactgg cgataccacc
    15181 tggggaacgg tattgttagt gtggagcggt gtggttggca cgctggataa cgtcatccgc
    15241 ccaatgttaa ttcgcatggg tgccgattta ccgctgatcc tgatcctctc tggtgttatt
    15301 ggtggtttga ttgctttcgg gatgatcggt ctgtttattg gtccggttct gttagccgtt
    15361 tcctggcgtc tgtttgccgc gtgggtggaa gaagtcccgc cgccgacgga ccaaccggaa
    15421 gaaattctcg aagaacttgg cgaaatcgag aaaccgaata agtaatttct catcaggcgg
    15481 ctctgccgcc tgattgttaa cccccgctaa ttgatatttg cttgctcttc cccatatttt
    15541 cctgcttacc gccattcagc tggtagtacc tgtcgcaaat tctttacagt ttttaaacta
    15601 atgagacgaa tctgatcgac gcaaaaagtc cgtatgccta ctattagctc acggttataa
    15661 atcaacatat tgatttataa gcatggaaat cccctgagtg aaacaacgaa ttgctgtgtg
    15721 tagtctttgc ccatctccca cgatgggctt ttttttaaca tttttccact tcgctacctc
    15781 gcccctcact ctttccaata atgttgcaca agatactgtg actctcttca agccgtcagc
    15841 aggctgataa tgttatttat attttattgt ttatttcatg cagccattac acgatgaacg
    15901 cttatgaact ccaggcactc cgacatattt ttgccatgac tattgatgaa tgcgcacctg
    15961 gattgcccag accggtgaca gtgaaagctg gcgtcagtgg gaaaatggca agtgtgccat
    16021 tcctgatcgt gtagtcgagc aactgttggc tatgcgtcag caaagaaaaa aacatcttca
    16081 tgccatcata gagaaaataa ataaccgcat cggcaacaac accatgcgtt ttttccccga
    16141 cttaaccgca tttcaacgag tctaccctga tggcaatttt attgactgga agatctatca
    16201 atcggtagcc gctgaaggta atgctgttga aaagcaaatt ccccagccag ttggtggacg
    16261 ccagcgtagc taatgaatta ccgcaaatga acagcaaacc gttagtctgg ctggaaggtg
    16321 tttcatcggt actgttcggt gtagccgcat tctcgacctt ttatgtgatt gtggtgtgga
    16381 tgcccaaata tgcgatggct tttgctggta tgtcagaagc tgaggcatta aaaaccatct
    16441 cttattacag tatgggctcg ttggtctgtg tctttatttt tgccgcacta ctgaaaaaaa
    16501 tggtccggcc catctgggct aatgtattta actctgcact ggcaacaata acagcagcca
    16561 ttatctatct gtacccttct ccactggtgt gtaatgccgg cgcctttgtt atcggtttct
    16621 cagcagctgg cggcatttta cagctcggtg tttcggtcat gtcagagttt ttcccaaaaa
    16681 gcaaagccaa agtcaccagt atttatatga tgatgggtgg actggctaac tttgttattc
    16741 cgctgattac cggttatctg tcgaacatcg gccttcaata tatcattgtt ctcgatttta
    16801 ctttcgcgct gctggccctg attaccgcaa ttattgtttt tatccgctat taccgcgttt
    16861 ttattattcc tgagaatgat gtgcggtttg gcgagcgtaa atttagcacc cggttaaaca
    16921 caattaagca tagaggttaa aggagttaat tatggatgtt accgcaaaat acgaattgat
    16981 tgggctgatg gcctatccta tccgccacag tttatcgcct gaaatgcaga ataaagcctt
    17041 agaaaaagcg ggattgccat ttacctatat ggcctttgaa gtggataacg atagttttcc
    17101 cggagcaatt gaaggattaa aagctctaaa aatgcgcgga actggtgtat caatgccgaa
    17161 caaacaactg gcttgttaat atgttgatga attaacgcca gcagccaaac tggtgggtgc
    17221 catcaacacc atcgttaatg atgatggcta cctgcgcggc tataacaccg acggcacggg
    17281 ccatattcgc gccattaaag agagcggttt tgatatcaaa ggcaaaacaa tggtgctgtt
    17341 aggggccgga ggtgcctcaa cggctattgg cgcgcagggg gcaattgaag gtttaaaaga
    17401 aattaaactc tttaaccgtc gggatgagtt cttcgataaa gccctcgcct tcgcgcagcg
    17461 ggttaacgaa aacaccgatt gtgtcgtcac ggttaccgat ctcgccgatc agcaagcctt
    17521 tgctgaagcc ctggcttccg ccgacatttt aaccaatggc acaaaagtgg gtatgaaacc
    17581 ccttgagaat gaatcattag ttaatgatat cagtctgtta catccgggac ttctggtcac
    17641 tgaatgcgtg tataacccgc atatgacgaa gttattgcag caggcgcaac aagctggttg
    17701 caaaacgatt gatggatacg gcatgttgtt gtggcaaggg gctgaacagt tcacgttatg
    17761 gactggcaaa gatttccctc tggaatatgt taaacaggtc atggggttcg gtgcctgaca
    17821 ggctgaccgc gtgcagaaag ggtaaaaaaa tgaaaaccgt aactgtaaaa gatctcgtca
    17881 ttggtacggg cgcacctaaa atcatcgtct cgctgatggc gaaagatatc gcccgcgtga
    17941 aatccgaagc tctcgcctat cgtgaagcgg actttgatat tctggaatgg cgtgtggacc
    18001 actttgccga cctctccaat gtggagtctg tcatggcggc ggcaaaaatt ctccgcgaaa
    18061 ccatgccaga aaaaccgctg ctgtttacct tccgcagtgc caaagaaggc ggcgagcagg
    18121 cgatttccac cgaggcttat attgctctca atcgtgcagc catcgacagc ggcctggttg
    18181 atatgataga tctggagtta tttaccggcg atgatcaggt taaagaaacc gtcgcctgcg
    18241 cccacgcgca tgatgtgaaa gttgtcatgt ccaaccatga cttccataaa acgccggaag
    18301 ccgaagaaat cattgcccgt ctgcgcaaaa tgcagtcctt cgacgccgat attcctaaga
    18361 ttgcgctgat gccgcaaagt accagcgatg tgctgacgtt gcttgccgcg accctggaga
    18421 tgcaggagca gtatgccgat cgtccaatca tcacaatgtc aatggcaaaa actggcgtaa
    18481 tttctcgtct ggctggtgaa gtatttggct cggcggcaac ttttggtgcg gtaaaaaaag
    18541 cctctgcgcc agggcaaatc tcggtaaatg atttacgcac agtattaact attttacatc
    18601 aggcataagc aataatattt cggcgggaat atcctccccc gccgaactaa aaaaaatata
    18661 ttcaaccgta tttaataaaa atatttcgtg agtctctgtg cgctaaatat ccatctggcg
    18721 tagggaaaat cacacctgaa tcagaaatta acaatgaaac ctgtaaaacc acctcgtatt
    18781 aatggacgag tgccggtcct gtcggcacag gaagcggtga attatattcc cgacgaagca
    18841 acactttgtg tgttaggcgc tggcggcggt attctggaag ccacgacgtt aattactgct
    18901 cttgctgata aatataaaca gactcaaacg ccacgtaatt tatcgattat tagtccaaca
    18961 gggcttggcg atcgcgccga ccgtggtatt agtccgctgg cgcaagaagg tctggtgaaa
    19021 tgggcattat gtggtcactg gggacaatcg ccgcgtattt ctgaactcgc agaacaaaat
    19081 aaaattattg cttataacta tccacaaggt gtacttacac aaaccttacg cgccgccgca
    19141 gcccaccagc ctggtattat tagtgatatt ggcatcggga catttgtcga tccacgccag
    19201 caaggcggca aactgaatga agtcacgaaa gaagacctga ttaaactggt cgagtttgat
    19261 aacaaagaat atctctatta caaagcgatt gcgccagata tcgccttcat tcgcgctacc
    19321 acctgcgaca gtgaaggcta cgccactttt gaagatgagg tgatgtatct cgacgcattg
    19381 gttattgccc aggcggtgca caataacggc ggtattgtga tgatgcaggt gcagaaaatg
    19441 gttaagaaag ccacgctgca tcctaaatct gtccgtattc cgggttatct ggtggatatt
    19501 gtggtggtcg atccggatca aagccaactg tatggcggtg cgccggttaa ccgctttatt
    19561 tctggtgact tcacccttga tgacagcacc aaacttagcc tgcccctaaa ccaacgtaaa
    19621 ttagttgcga ggcgtgcatt attcgaaatg cgtaaaggcg cggtggggaa tgtcggcgtc
    19681 ggtattgctg acggtattgg cctggtcgcc cgggaagaag gttgtgctga tgactttatt
    19741 ctgacggtag aaacaggtcc gattggcgga attacttcac aggggctcgc ctttggcgcg
    19801 aacgtgaata cccgtgccat tctggatatg acgtcccagt ttaattttta tcacggtggt
    19861 ggtctggatg tttgttattt gagttttgct gaagtcgacc agcacggtaa cgtcggcgtg
    19921 cataaattca atggcaaaat catgggtacc ggtggattta ttgatatcag tgccacctcg
    19981 aagaaaatta ttttctgcgg cacgttaacc gcgggcagtt taaaaacaga aattgccgat
    20041 ggcaaattaa atatcgtcca ggaaggacgg gtgaagaaat ttattcagga actaccggaa
    20101 attactttta gcggaaaaat cgtgctcgaa cgggaactgg atgttcgtta catcaccgaa
    20161 cgtgctgtat tcacgctgaa agaagacggc ctgcatttaa tcgaaatcgc ccctggcgtc
    20221 gatttacaaa aagatattct cgacaaaatg gatttcaccc cagtgatttc gccagaactc
    20281 aaactgatgg acgaaagatt atttatcgat gcggcgatgg gttttgtcct gcctgaagcg
    20341 gctcattaac aggagtatca cgatggattt ttctttaact gaagaacaag aactgctgct
    20401 ggccagtatt cgcgaactga ttacgactaa ctttgcggaa gagtatttcc gcacctgcga
    20461 tcaaaacggg acatatccgc gtgagtttat gcgggcgctg gcggataacg gcatttctat
    20521 gcttggcgtg ccggaagagt tcggtggcat ccctgcggat tacgtcaccc agatgctggc
    20581 gctgatggaa gtgtcaaaat gcggtgctcc ggcatttttg attaccaacg gtcaatgtat
    20641 tcacagtatg cgccgtttcg gttctgcaga gcagctacgt aaaacggcag agagcaccct
    20701 ggaaacgggt gatcccgcct atgccctggc gttgacggag ccaggcgcag gctcagacaa
    20761 caacagtgcc actaccactt acacgcgtaa aaacggcaag gtttacatca acggacagaa
    20821 aacctttatt accggtgcga aagagtaccc gtatatgctg gtgttggcgc gcgatccgca
    20881 accgaaagat cccaaaaaag ccttcaccct gtggtgggtc gactccagta agcccggcat
    20941 taagattaac ccgctgcata aaatcggctg gcatatgctc agcacctgcg aagtctatct
    21001 cgacaacgtg gaggttgaag agagcgacat ggtgggcgaa gaaggaatgg gtttcctcaa
    21061 tgtgatgtac aactttgaga tggagcgcct gatcaacgcc gcgcgcagca ccggctttgc
    21121 cgaatgcgcc tttgaagatg ccgcccgcta tgccaaccaa cgtatcgctt ttggtaagcc
    21181 cattggtcat aaccagatga tccaggaaaa actggcgctg atggcgatta agatcgacaa
    21241 catgcgcaac atggtgctga aagtggcatg gcaagccgat cagcatcagt cactgcgcac
    21301 cagcgcggcg ctggcaaaac tgtattgcgc acgtaccgca atggaagtca ttgatgatgc
    21361 gattcaaatc atgggcggtc tgggctatac cgatgaagcg cgcgtctccc gcttctggcg
    21421 tgatgtccgt tgtgaacgta tcggcggcgg tacagacgaa attatgattt acgtagcagg
    21481 tcggcagatc ctgaaagact atcagaacaa ataatctgca ggcggcgcag cttcttaaca
    21541 aactgcgccg ccagatttat ccaacaagac ttaccggttg aggaaattca gataggattt
    21601 cttgttccgc aaaacagttt ttaaattgtc tacggtaatc gctgggcgaa caaccgacat
    21661 ggcgcaaaaa cagtttggca aagtgatcga cattttcata acccacacgc caggagatct
    21721 ctgcctgtga taattcagta ttagtgagtg accattttgc ttccgtcata cgccgttgaa
    21781 taacatagtt aataggggaa atacgatact ctttggtaaa ttcatggcaa atataactga
    21841 cgctggcacg aaattttttc gataactgtt ctaaagtgat tttttcgcga taattattac
    21901 tcagatagaa aagaatatct tttatcagaa catctttttt aatatacccc tgctccgaac
    21961 gataagcatt tttaaaattt tcgtagtaaa gaatggctaa cgtataggca aatgcatccc
    22021 ataacgaaga tgtttggcta tttttacttt gcggcaaaat cacacttagc tcattaaata
    22081 aggtttttat gacttctttt ccctgccccg cggcaattac cggacaagaa tgcggttgca
    22141 gtagctgatt ttcctcagcc ccctgaaact gaaagccgta cagcgcacag gtacacgtcg
    22201 ttgccggatc gttaacgtca gaggccaccg catgcagcct gccgcgttct atcaccacaa
    22261 tgtcatccgc atgtgcgaca tataacgaag aatcgatggt aaaccttgca acccctttct
    22321 tgacgtaaat caactcggtt tcattgtcat gaacgtgatg tccggactcc cattttggat
    22381 catcgctaaa tgcaaaacgt gaaagccgtg gcgttttacc ggcgacaaac agcgtttcac
    22441 tggcgttatc aaaacagcgt tgatacatga caacctccct attccatgag caagcaaaaa
    22501 caatatatgc cggataaaat acggcgtgta ttgcggttat acaaccgcgt ttagcgagag
    22561 ctaaacaaga ttatttacac tgaatgcaag attgtacggt catggaataa ctatattgcc
    22621 gcaaattgca cttttgtttt agcgatcgca tttttttgca agattgttgg caaggaaaac
    22681 agcttgctcc gtcgaaaacc cccaccgcta tcgcacacta ttttcaggcc atttttacct
    22741 tccatcggag atggttccgt atgcgactca caggagaaat catgaaaata ataacctgct
    22801 ttaagctggt gcctgaagaa caggacattg ttgtcactcc agaatacacc ctgaatttcg
    22861 acaatgccga cgccaaaatc agccagttcg atctcaatgc cattgaagct gcaagccagc
    22921 tcgcaacaga tgacgatgag atagccgcgc tgaccgttgg cggctcattg ttgcagaact
    22981 cgaaagtgcg cagtgcgcaa agatgtgcta tcccgcgggc cgcacagcct gtatttggtg
    23041 caggatgcgc aacttgaaca tgcactgcct ctcgataccg caaaagcgct ggcggcagca
    23101 attgaaaaga tcggcttcga tttactgatc tttggtgaag gttccggcga cctttatgcc
    23161 cagcaggttg gcttgctggt cggagaaatt ctgcaacttc cggtgattaa tgcagtgagt
    23221 gctattcagc gtcagggtaa tacactggtg attgaacgca cgcttgaaga tgatgttgaa
    23281 gttattgaac tctctgttcc agccgtgctc tgcgtcacct ccgatattaa cgtgccacgc
    23341 attccttcga tgaaagccat tctcggcgcg ggtaaaaaac cggtaaatca gtggcaggca
    23401 agtgatattg actggagcca gagcgcgcca cttgctgaac tggtcggcat tcgcgtaccg
    23461 ccgcaaacag aacgtaagca catcattatc gataacgatt cgccggaggc cattgccgag
    23521 ctggcggaac atctgaagaa agccctgaac tgaagcctac ggagaagaaa cgatgagtca
    23581 attaaacagc gtctgggtct ttagcgataa tcctgaacgt tatgctgaac tgtttggcgg
    23641 cgctcagcaa tggggccaac aggtgtatgc cattgtacaa aataccgacc aggcgcaggc
    23701 agttatgcct tatggtccaa aatgtattta tgttcttgag caaaacgacg cgctgcaacg
    23761 cactgaaaat tacgccgaaa gcattgctgc cccgctgaaa gataaacacc ccgctatgtt
    23821 gctgttggcc gcgacgaaac gtggtaaagc tctggcagca cggttaagtg tgcaactgaa
    23881 tgcggcgctg gtgaacgatg ccacggcggt ggatattgtc gatggtcaca tttgcgccga
    23941 acaccggatg tatggcgggt tagcgttcgc tcaggaaaag atcaacagcc cgctggcgat
    24001 cattaccctt gcacccggtg ttcaggaacc gtgcaccagt gatacctctc atcagtgccc
    24061 gacagaaacg gtaccttatg ttgctccgcg tcatgaaatt ctctgtcgcg aacgccgtgc
    24121 gaaagccgca agcagcgtgg acctgagcaa agcaaaacgt gtggttggcg tcggtcgtgg
    24181 tctggcggcg caggatgacc tgaaaatggt ccacgaactg gcggcggtgc tgaatgctga
    24241 agtcggctgt tcacgtccaa ttgccgaagg cgagaactgg atggagcgtg aacgttatat
    24301 cggtgtctcc ggcgtgttgc tgaaatccga tctctacctg acgctgggga tctccgggca
    24361 gatccagcat atggttggcg gcaacggcgc aaaagtgatt gtcgccatca ataaagataa
    24421 aaatgcgcca atcttcaatt atgccgacta cggtctggtg ggcgatatct acaaagtcgt
    24481 ccctgccctg attagccagt tgagccgcta attccccttt caaccacatc tcccgctgta
    24541 aaggcagcgg gaagggagca taacgcatgt cggatgacaa atttgatgcc attgtggtcg
    24601 gtgcggacgt tgctagtagc gttgccgcac tggtcatggc gcgagccgga ctggatgtcc
    24661 tggtgataga acgcggcgac agtgccggat gtaaaaacat gaccggcggg cgtctttatg
    24721 cccacacact tgaagcaatc attccaggct ttgcagtatc agcgccggta gaacgcaagg
    24781 tcacacgcga gaaaatctcc ttcttaaccg aagaaagcgc cgttaccctc gattttcacc
    24841 gcgagcaacc agatgttccg caacacgcat cttataccgt attgcgtaat cgtctggacc
    24901 cgtggttgat ggaacaagcc gagcaggcgg gcgcacagtt tatcccgggc gttcgcgtcg
    24961 atgcgttggt tcgtgaagga aacaaggtca ctggcgtgca ggccggggat gatattctcc
    25021 aagcgaatgt ggtggttcta gctgatggcg taaactcgat gcttggccgc tcgctgggaa
    25081 tggttcccgc ttccgatccg catcattacg ctgttggtgt taaagaggtt attggcctca
    25141 caccagaaca gatcaacgat cgctttaata ttacgggcga ggaaggtgcc gcctggctgt
    25201 ttgccggctc cccttctgac ggcctgatgg gcgggggatt tctctatacc aacaaggatt
    25261 ccatatcctt ggggctggtt tgtggattgg gtgatatcgc ccatgcgcaa aaaagcgtgc
    25321 cgcaaatgct ggaagatttt aaacaacacc ccgccattcg cccactaatt agcggcggca
    25381 aactgcttga atattccgcg catatggtgc cagaaggcgg tctggcaatg gtgccgcaga
    25441 tggttaacga tggcgtgatg atcgttggtg acgccgcagg cttctgcctg aatttgggtt
    25501 ttacagttcg tggcatggat ttagccattg catcggctca ggctgccgcc acaacggtga
    25561 tcgccgccaa agaacgcgag gatttctccg ccagcagtct ggcgcaatac aaacgtgagc
    25621 tggaacaaag ctgcgtcatg cgcgatatgc agcatttccg caagatcccg gcgctgatgg
    25681 aaaacccgcg cctgtttagc caatactcac gaatggtcgc cgacatcatg aacgagatgt
    25741 tcaccattga cggcaaacca aaccagccgg tacgcaaaat gatcatggga cacgcgaaga
    25801 aaattgggct gatcaacttg ctgaaagatg gcattaaggg agcaaccgcg ctatgagcca
    25861 gaacgctacg gttaacgtcg acatcaaatt aggcgtcaat aaattccatg ttgatgaggg
    25921 ccacccgcat atcattctgg cggcaaatcc cgatatcaat gaattccgta aattaatgaa
    25981 agcttgccct gccggacttt ataagcagga tgacgccggc aacattcatt tcgattccgc
    26041 cggttgtctg gagtgcggca cctgtcgggt gctgtgcggc aacactattc tcgaacagtg
    26101 gcaatatccc gcaggcacct tcggtattga ttttcgctac ggctaatcat gcatcccaca
    26161 ggcccgcatc tcgggcctga tgttctgttt cgagagtcca aaatgaaagt gacattaacg
    26221 ttcaacgaac aacgtcgtgc ggcgtatcgt cagcaagggt tatggggcga tgcttcactg
    26281 gccgattact ggcagcagac cgctcgtgcg atgccagaca aaattgccgt ggtcgacaat
    26341 catggtgcaa cgtacaccta tagcgcgctc gatcacgccg cgagctgtct ggcaaactgg
    26401 atgttagcga agggtattga atcaggcgat cgcatcgcat ttcaactgcc tggctggtgt
    26461 gaatttaccg ttatctatct tgcctgcctg aaaatcggtg cggtttccgt gccgctgttg
    26521 ccttcctggc gggaagcaga actggtgtcg gtgctcaata agtgtcaggc aaaaatgttc
    26581 tttgcaccga cgttgtttaa acaaacacgt ccggtagatt taatcctgcc gctgcaaaat
    26641 cagcttccac aactacaaca aattgtcggc gtggacaaac tggctcccgc cacctcttcc
    26701 ctctcattaa gtccgattat cgccgacaat acctcactga ccacggcgat aacgacccac
    26761 ggcgatgaat tagctgcggt gctgtttacc tccggaaccg agggtctgcc aaagggcgtg
    26821 atgctaacgc ataacaatat tctcgccagt gagcgggctt attgcgcgcg actgaatctg
    26881 acctggcagg atgtctttat gatgcctgcg ccacttggtc acgcaacggg ctttctgcat
    26941 ggcgtaacgg caccattctt aattggcgct cgcagcgtgt tgttagatat tttcactcct
    27001 gatgcgtgtc tcgcgctgct tgagcagcag cgttgcacct gtatgctagg cgcaacgccg
    27061 tttgtctatg atcttttgaa tgtactagag aaacaacccg cggacctttc agcgctgcgt
    27121 ttctttcttt gcggcggtac cacaatcccc aaaaaagtgg cacgtgaatg ccagcagcgc
    27181 ggcattaaat tattaagtgt ttatggttcc acagaaagtt cgccgcatgc ggtggtgaat
    27241 ctcgatgatc ctttgtcgcg ctttatgcac accgatggtt acgctgccgc aggtgtagag
    27301 attaaagtgg tcgatgacgc acgcaagacc ttaccgccag gttgcgaagg tgaagaagcc
    27361 tcgcgtggcc ccaatgtgtt tatggggtat tttgatgaac ctgaattaac cgcccgtgcg
    27421 ctggacgaag aaggctggta ttgcagcggc gatctctgcc gtatggatga ggctggctat
    27481 ataaaaatta ccggacgcaa aaaagatatt attgtccgcg gcggcgaaaa tattagcagc
    27541 cgtgaagtgg aagatatttt attgcagcat cctaaaattc acgatgcctg tgtggttgca
    27601 atgcccgatg aacgtttagg tgaacgatca tgcgcttatg tcgtgctgaa agcgccgcat
    27661 cattcattat cgctgg
//
LOCUS       DADLFA010000035        27525 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000035 DADLFA010000000
VERSION     DADLFA010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27525
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.035"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(172..702)
                     /gene="ppa"
                     /locus_tag="JQH65_001284"
     CDS             complement(172..702)
                     /gene="ppa"
                     /locus_tag="JQH65_001284"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HAY6138758.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            1012..1968
                     /gene="ytfQ"
                     /locus_tag="JQH65_001285"
     CDS             1012..1968
                     /gene="ytfQ"
                     /locus_tag="JQH65_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HAY6138759.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENRIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            2108..2644
                     /locus_tag="JQH65_001286"
     CDS             2108..2644
                     /locus_tag="JQH65_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAY6138760.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQVISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDT"
     gene            2648..3610
                     /locus_tag="JQH65_001287"
     CDS             2648..3610
                     /locus_tag="JQH65_001287"
                     /inference="COORDINATES: protein motif:HMM:NF012235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAY6138761.1"
                     /translation="MELLFGLMRQLRDRGVSLIFVTHFLDQSYQVSDRITVLRNGSFV
                     GCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTIAPFDLE
                     VRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQNLRSPHQASVLGIGFC
                     PEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIRTPSTEQ
                     PIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCADGLALL
                     VISSELEELVGYADRVIIMRDRKQVAEIRLAELSVPAIMNAIAA"
     gene            3624..4646
                     /locus_tag="JQH65_001288"
     CDS             3624..4646
                     /locus_tag="JQH65_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY6138762.1"
                     /translation="MPQSLPDTTTPKRRFRWPTGMPQLVALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     EVSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            4633..5628
                     /gene="yjfF"
                     /locus_tag="JQH65_001289"
     CDS             4633..5628
                     /gene="yjfF"
                     /locus_tag="JQH65_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HAY6138763.1"
                     /translation="MIKRNLPLMITIGVFVLGYFYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVVGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(5661..6659)
                     /gene="fbp"
                     /locus_tag="JQH65_001290"
     CDS             complement(5661..6659)
                     /gene="fbp"
                     /locus_tag="JQH65_001290"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HAY6138764.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            6835..8208
                     /gene="mpl"
                     /locus_tag="JQH65_001291"
     CDS             6835..8208
                     /gene="mpl"
                     /locus_tag="JQH65_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HAY6138765.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADTANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(8291..8842)
                     /locus_tag="JQH65_001292"
     CDS             complement(8291..8842)
                     /locus_tag="JQH65_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HAY6138766.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEE"
     gene            8936..10288
                     /gene="pmbA"
                     /locus_tag="JQH65_001293"
     CDS             8936..10288
                     /gene="pmbA"
                     /locus_tag="JQH65_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HAY6138767.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGVDCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            10472..10858
                     /gene="cybC"
                     /locus_tag="JQH65_001294"
     CDS             10472..10858
                     /gene="cybC"
                     /locus_tag="JQH65_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HAY6138768.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEGKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(10903..11367)
                     /gene="nrdG"
                     /locus_tag="JQH65_001295"
     CDS             complement(10903..11367)
                     /gene="nrdG"
                     /locus_tag="JQH65_001295"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HAY6138769.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(11466..13604)
                     /gene="nrdD"
                     /locus_tag="JQH65_001296"
     CDS             complement(11466..13604)
                     /gene="nrdD"
                     /locus_tag="JQH65_001296"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004128226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAY6138770.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYSNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(13997..15652)
                     /gene="treC"
                     /locus_tag="JQH65_001297"
     CDS             complement(13997..15652)
                     /gene="treC"
                     /locus_tag="JQH65_001297"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAY6138771.1"
                     /translation="MTNPPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAVLKKVCEFWADRGVDGLRLDVVNLISKDPRFPDDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(15702..17123)
                     /gene="treB"
                     /locus_tag="JQH65_001298"
     CDS             complement(15702..17123)
                     /gene="treB"
                     /locus_tag="JQH65_001298"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HAY6138772.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNARQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAETFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPNYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(17224..18189)
                     /gene="treR"
                     /locus_tag="JQH65_001299"
     CDS             complement(17224..18189)
                     /gene="treR"
                     /locus_tag="JQH65_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HAY6138773.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPEFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLSRSFPER"
     gene            18374..18427
                     /gene="mgtL"
                     /locus_tag="JQH65_001300"
     CDS             18374..18427
                     /gene="mgtL"
                     /locus_tag="JQH65_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HAY6138774.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            18568..21264
                     /gene="mgtA"
                     /locus_tag="JQH65_001301"
     CDS             18568..21264
                     /gene="mgtA"
                     /locus_tag="JQH65_001301"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAY6138775.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQLSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(21470..21856)
                     /gene="ridA"
                     /locus_tag="JQH65_001302"
     CDS             complement(21470..21856)
                     /gene="ridA"
                     /locus_tag="JQH65_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HAY6138776.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAIRR"
     gene            complement(21929..22390)
                     /gene="pyrI"
                     /locus_tag="JQH65_001303"
     CDS             complement(21929..22390)
                     /gene="pyrI"
                     /locus_tag="JQH65_001303"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HAY6138777.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(22403..23338)
                     /gene="pyrB"
                     /locus_tag="JQH65_001304"
     CDS             complement(22403..23338)
                     /gene="pyrB"
                     /locus_tag="JQH65_001304"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HAY6138778.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(23342..23476)
                     /gene="pyrL"
                     /locus_tag="JQH65_001305"
     CDS             complement(23342..23476)
                     /gene="pyrL"
                     /locus_tag="JQH65_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HAY6138779.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(23756..24151)
                     /locus_tag="JQH65_001306"
     CDS             complement(23756..24151)
                     /locus_tag="JQH65_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HAY6138780.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     SYPTWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(24282..24995)
                     /gene="bdcA"
                     /locus_tag="JQH65_001307"
     CDS             complement(24282..24995)
                     /gene="bdcA"
                     /locus_tag="JQH65_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY6138781.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADKDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTYANPANGPMRDMSHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            25066..25659
                     /locus_tag="JQH65_001308"
     CDS             25066..25659
                     /locus_tag="JQH65_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY6138782.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGNKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            25804..26256
                     /locus_tag="JQH65_001309"
     CDS             25804..26256
                     /locus_tag="JQH65_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF386 domain-containing protein"
                     /protein_id="HAY6138783.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPHEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPARVRKAVVKMLMA"
     gene            26379..27525
                     /locus_tag="JQH65_001310"
                     /pseudo
     CDS             26379..27525
                     /locus_tag="JQH65_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234052.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT         6606 a         6812 c         7280 g         6827 t
ORIGIN      
        1 cagcaagtgg ctatgaacag caaaatggtg gtcgggtttg cttgttctta ccgttcaagc
       61 cttgcaaccg ctgagaataa tgatggtgga atagagagtt tatttatcgc gggcataaaa
      121 aaacccttac taaccgaagc ccggcgttca gggttattgc gctagaagaa cttatttatt
      181 ctttgcgcgc tcgaaggaag caacgatttc agctttagcg gcttctgcgt tttcccaacc
      241 ttcaactttc acccacttgc ctttttcgag gtctttgtag tgctcgaaga agtgagcgat
      301 ttgcgctttc agcagttcag gcagatcgtt tacgtcttta atgtgatcgt attctttgct
      361 cagcttgctg tgcggaaccg caaccagttt cgcatcttca ccggcttcgt cggtcatttt
      421 cagaacgcca accggacggc aacggatcac agaaccaggc tgcagcgggt acggagtcgg
      481 gaccagtacg tcaaccgggt caccgtccag agacagggtg tggttgatgt aaccgtagtt
      541 gcacggatag aacatcgcgg tggacatgaa gcggtcaacg aacagtgcgc cgctctcttt
      601 gtcgatttcg tatttgatcg gatctgcgtt agccgggatc tcaataacaa cgtagatgtc
      661 ttccggcaga tctttacccg cagggacgtt gagtaagctc atgtctgttt cctttaaaaa
      721 tatgtggcaa acaagtgccg agtattatag ccaactcgcg ccgaatgtct ttgcttgttt
      781 tcgtcttcat tttccttttt cgccagtttt aagacggtat acagagcagg aaaatccata
      841 accgtagccg catttttcat agtgagatga aagcgattac aaacttgtga ttaacgtttt
      901 atttactttt ttgaagtgtg atgtaacgca atccgttaca taacgaattg tctatagttt
      961 tttcgcgaac atcttttcac caataataat taccccgacg aggacaaccc tatgtggaaa
     1021 cgcttacttg tagtctctgc agtctcggca gccatgtcgt ctatggcgtt ggccgctcca
     1081 ttaaccgtag gattttcgca ggtcggatcg gaatccggct ggcgcgccgc agaaaccaat
     1141 gtggcgaaaa gtgaggccga aaaacgcgga attacgttga aaattgccga tggtcagcaa
     1201 aagcaggaaa accggattaa agcggtacgt tccttcgttg cacaaggggt ggatgcgatc
     1261 tttatcgctc cagtggttgc gacgggttgg gaaccggtat taaaagaggc gaaagatgcc
     1321 gaaatcccgg tcttcttgct cgaccgttcc atcgatgtga aagacaaatc tctctatatg
     1381 accaccgtta ctgccgacaa catcctcgaa ggtaagttga tcggtgactg gctggtaaaa
     1441 gaagtgaatg gcaaaccatg caacgtggtg gagctgcagg gcaccgttgg ggccagcgtc
     1501 gccattgacc gtaagaaagg ctttgccgaa gccattaaga atgcgccaaa tatcaaaatt
     1561 atccgctcgc agtcaggtga cttcacccgc agtaaaggca aagaagtcat ggagagcttt
     1621 atcaaagcgg aaaacaacgg caaaaacatc tgcatggttt acgcccataa cgacgacatg
     1681 gtgattggtg caattcaggc aattaaagaa gcgggcctga aaccgggcaa agatattctg
     1741 acaggttcta tcgatggcgt accggatatc tacaaagcga tgattgatgg cgaagcgaac
     1801 gccagtgttg aactgacgcc gaatatggcg ggacccgctt ttgacgcgct ggagaaatac
     1861 aaaaaagacg gcaccatgcc tgaaaagctg acgttaacca aatccaccct ttacctgcct
     1921 gataccgcaa aagaagagtt agagaagaag aaaaatatgg ggtattgagg gttgctatgc
     1981 ctgatgccga ttcgtaggcc ggataaggcg ctcgcgccgc atccggcgat ggtgcactga
     2041 agcctgatgc gacgcttacc gcgtcttatc atgcctactg ggagcacgct ttacaccggg
     2101 ggaaaccatg acgaccgacc aacaccagga aatcctccgc accgaaggat taagtaaatt
     2161 tttccccggc gtcaaagcgt tagacaacgt tgatttcagc ctgcgccgtg gcgaaatcat
     2221 ggcgctgctc ggtgaaaacg gggcgggaaa atcaacgcta atcaaagcat taactggtgt
     2281 ataccacgcc gatcgcggca ccatctggct ggaaggccag gttatctcac cgaaaaatac
     2341 cgcccacgcg caacaactcg gcattggcac cgtctatcag gaagtcaacc tgctacccaa
     2401 tatgtcggtc gctgataatc tatttatagg ccgcgaaccc aaacgcttcg gccttctacg
     2461 ccgcaaagag atggaaaagc gcgccaccga actgatggca tcttacggtt tctccctcga
     2521 cgtgcgcgaa ccgctcaacc gcttttcagt cgcgatgcag caaatcgtcg ccatttgccg
     2581 ggctatcgat ctctccgcca aagtgctgat cctcgatgaa cccaccgcca gcctcgacac
     2641 ctaggaagtg gagctattat ttggcctgat gcgtcagttg cgcgatcgcg gcgtcagcct
     2701 gatctttgtc actcactttc tcgatcagag ctatcaggtc agcgatcgga tcaccgtttt
     2761 acgcaacggc agtttcgtag gctgtcggga aacgcgcgag ctaccgcaga tcgaactggt
     2821 aaaaatgatg ctggggcgcg agctggatac ccacgcgcta cagcgtgccg ggcgaacatt
     2881 gttgagcgac aaacccgttg ccgcgttcaa aaattacggc aaaaaaggaa cgatagcacc
     2941 gtttgatctc gaagtgcgcc ccggcgagat cgtcggcctg gcaggcttgc tgggatccgg
     3001 acgtaccgaa accgccgaag tgatcttcgg tatcaaacct gctgacagcg gcacggcgtt
     3061 gatcaaaggc aaaccgcaaa acctgcgatc gccacatcag gcttcggtac tgggcatcgg
     3121 cttctgcccg gaagacagga aaaccgatgg catcatcgct gccgcctcgg tgcgggaaaa
     3181 tatcatcctc gctctccagg cccagcgcgg ctggctacga cctatttccc gcaaagagca
     3241 gcaagagatt gccgaacgct ttatccgcca gcttggcatt cgcacacctt caactgaaca
     3301 accgattgaa tttctctccg gcggcaatca gcaaaaagtg ttgctttcac gttggctact
     3361 gacccgaccg caatttctga tcctcgatga gccaacccgt ggcattgatg ttggtgccca
     3421 cgccgagatc atccgcctga ttgaaacgct atgcgccgat ggtctggcgc tgctggtgat
     3481 ctcctccgaa ctggaagaac tggtgggcta tgccgaccgg gtgatcatca tgcgcgatcg
     3541 caaacaggtg gcggagatcc ggctggcaga gctttccgtt ccggctatca tgaacgccat
     3601 tgcggcgtaa ggagaacagt gtgatgcctc aatctcttcc ggacaccact acgccaaaaa
     3661 ggcgctttcg ctggccgacg gggatgccgc agctggtagc actattgctg gtgctgctgg
     3721 tcgacagcct ggtagccccg catttctggc aggtagtgct tcaggatggg cgtttgttcg
     3781 gtagccccat agacattctt aaccgtgcag cccccgttgc gttactggcg attggtatga
     3841 cgctggtgat cgccaccggt gggattgatc tctccgtagg ggcggtgatg gctatcgccg
     3901 gagccacaac ggctgcgatg acggtcgcgg aagtcagcct gccgattgtt ttgttaagcg
     3961 ccctaggcac cggcatcctg gcgggattgt ggaacggcat actggtagcg atcctcaaaa
     4021 ttcagccatt tgtcgccacc ctgatcctga tggtcgccgg gcgcggcgtg gcgcaactga
     4081 tcacctccgg acagatcgtc acgtttaact cgccggatct ctcatggttt ggcagtggat
     4141 cgctgttgtt cctgccaacg ccagtcatta ttgcggtact gacgcttctc ctgttctggc
     4201 tgttgacccg caaaacggca ctggggatgt ttatcgaagc cgttggtatc aacattcggg
     4261 cggcaaaaaa tgccggggta aacacgcgga tcatcgtcat gctcacttat gtgttgagcg
     4321 ggctgtgtgc ggcgattgcg ggcattatcg tggcggcgga tattcgcggt gccgatgcca
     4381 acaacgccgg attatggctg gagctggacg ccattctcgc ggtggtgatt ggcggcggat
     4441 cgctgatggg cgggcgcttt aacctgctgc tctcggtagt gggggcgctg attattcagg
     4501 ggatgaacac cggaattttg ctttcgggct ttccaccgga gatgaaccag gtggtgaaag
     4561 cggtggtggt gctttgcgtg ctgattgtcc agtcgcaacg ctttatcagt ctgattaaag
     4621 gagtacgtag ccgtgataaa acgtaatttg ccactgatga tcaccatcgg cgtctttgtg
     4681 ctgggctatt tctactgcct gacgcagttt cccggttttg cttccacaag agtgatctgc
     4741 aatatcctga ccgataacgc ttttctgggg attattgccg ttggtatgac ctttgtgatc
     4801 ctctctggcg gtatcgatct ctccgtcggt tcggtgatcg cctttactgg cgtgtttctg
     4861 gcaaaagtgg ttggcgattt cggcctctcg ccgctgctgg cgtttccgct agtgctggtg
     4921 atgggctgtg ccttcggcgc atttatgggg cttctgatcg acgccctgaa gatcccggca
     4981 tttatcatta cgcttgcggg gatgttcttt ttgcgcggcg tcagctatct cgtttcggaa
     5041 gagtcgattc cgataaacca tcccatttat gacacgctct caagccttgc gtggaaaatc
     5101 cccggcggcg gtcgccttag tgcgatgggg ctgttgatgc tggcggtggt ggttatcggt
     5161 attttccttg cgcatcgtac ccgttttggc aatcaggtgt acgctattgg cggcaacgcg
     5221 acgtcggcga acctgatggg gatttctact cgcagcacta ctattcgcat ttatatgctc
     5281 tccaccggac tggcaacact ggcggggatt gtcttctcga tttataccca ggccggatat
     5341 gcactggcgg gcgtaggtgt ggaactggac gccatcgcgt cggtggtgat tggcggtacg
     5401 ttgttaagtg gtggcgtcgg gacggtgctt gggacgcttt ttggcgtggc aattcaggga
     5461 ctgatccaga cttacatcaa ctttgacggc acgctgagtt cctggtggac gaaaatcgcc
     5521 atcggtattt tgttgtttat ttttatagca ttacagcgtg ggttaacggt gctgtgggag
     5581 aatcgtcaga gttcgccagt gacaagagtc aacattgcgc agcgataaaa atgcctctcc
     5641 gtgtggagag gcgcaggaga ttacgcgtcc gggaactcac ggataaagcg ttcgacatct
     5701 tcaaccatat ggtcgttgcc gacaaagaac gaacggcgct ggtgcagagt ttccgggatg
     5761 atatccagaa tacgctcttt gccatcgctc gctttaccgc ccgcttgttc cgccaggaat
     5821 gccatcgggt tgcactcata aagcaaacgc agtttgccgt ccgggtggct ggcggtgctt
     5881 gggtagagat aaataccgcc tttcagcagg ttacggtgga aatccgcaac cagtgaaccg
     5941 atataacgtg aggtatacgg gcggttggtg gatttatctt cttcctggca gaatttaatg
     6001 tacttcttca ccccgttcgg aaacttaatg tagtttcctt cgttgatgga gtaggttttg
     6061 cctttctccg ggaagcgcat ccgttcctgg cacaggcaga aaacgcccag cgaaggatcg
     6121 taagtaaagg cgtgaacacc gcatccggtg gtgtaaacca gcatggtaga ggagccgtat
     6181 accacgtaac ctgccgcaac ctgtttgtta ccaggctgga ggaaatcttc ttccgttacc
     6241 ggcgtgccga cgggcgtaac tcggcggtag atggagaaaa tggtaccgac agagacgtta
     6301 acatcgatgt tggacgagcc atccagtggg tccatcagca ccacgtattt tgcatgttca
     6361 cagccttcaa agacaacaat ctcatcttct tcttcagagg caatgcctgc aacgatatcg
     6421 cgtgctttca gtgcggcttt cagtttttca ttagcgaaca agtcgagttt ttgctgaacc
     6481 tcgccctgca cgttctcagc accgctggca cccaggatat caaccagtcc tgctttgttg
     6541 atatcgcgat ggataatctt ggcgcccagt tttattgccg acagcaaagc agtgagctca
     6601 ccggtagcat gagaaaactc gtgctgcttt tcgacaataa attcacctaa cgttttcata
     6661 aaactttccc tgcaatgttt atggagtaaa gcgaccgcaa caatcttaac aaataatctc
     6721 aatgttgcgc tcaggtgaat cgcgccagca aattacggat tatcctgaaa tgcgtttctc
     6781 acttgcccga catatgcgta aaatgagcgg cagattaaaa aaggatagtg acgtatgcgc
     6841 attcatattt taggaatttg tggcacgttt atgggcggtc tggcgatgct ggcgcgccag
     6901 ttaggccatg aagtaacggg ttcggacgcc aatgtgtatc cgccgatgag caccttactt
     6961 gagaagcaag gcattgaact gattcagggt tacgatgcca gccagctcga tccgcagccg
     7021 gatctggtga ttattggcaa cgccatgacc cgtggaaatc cgtgtgtgga agcggtactg
     7081 gaaaaaaaca tcccttatat gtcaggtcca cagtggctgc acgattttgt gctgcgcgac
     7141 cgctgggtgc tggccgttgc cggtacacac ggcaaaacca ccaccgcggg aatggcgacc
     7201 tggattctgg aacagtgtgg ttacaaaccg ggatttgtga tcggcggtgt gccggggaac
     7261 tttgaggttt cggcgcgtct gggcgaaagc gacttctttg ttatcgaagc ggatgagtac
     7321 gactgcgcct tcttcgacaa acgctctaaa tttgtccatt actgcccgcg tacgctgatc
     7381 ctcaacaacc ttgagttcga tcacgccgat atctttgacg acctgaaagc gatccagaaa
     7441 cagttccacc atctggtgcg tatcgttccg gggcagggcc gtattatctg gccggaaaat
     7501 gacatcaacc tgaaacagac catggcgatg ggctgctgga gcgagcagga gctggtgggt
     7561 gagcaggggc actggcaggc gaaaaagctg accactgatg cttccgaatg ggaagtcttg
     7621 ctggatggcg aaaaagtggg cgaagtgaaa tggtcgctgg taggcgaaca taatatgcat
     7681 aatggcctga tggcgattgc ggcggcccgc catgttggcg tagcgccggc agataccgct
     7741 aacgcgctgg gttcgtttat taatgctcgt cgccgtctgg agttgcgtgg tgaagcgaat
     7801 ggcgtcacgg tatatgacga ttttgcccat cacccgacgg cgattctggc aacgctggcg
     7861 gcgctgcgtg gcaaagttgg tggtacagcg cgcattattg ctgtgctgga accgcgctcg
     7921 aataccatga aaatggggat ctgcaaagac gatttggcac cttcattagg tcgtgccgat
     7981 gaagtcttcc tgctgcaacc ggcgcatatt ccgtggcagg tggcagaagt ggcagaagcc
     8041 tgcgttcagc ctgcacactg gagtggcgat gtggatacgc tggcagatat ggtggtgaaa
     8101 accgctcagc ctggcgacca tattctggtg atgagcaacg gcggttttgg tgggatccat
     8161 cagaaactgc tggatggtct ggcgaagaag gcggaagctg cgcagtaatt cggcctcagc
     8221 ctgagatagc attgccggat aaggcgttta cgccgcatcc ggcaaaccat actcacatca
     8281 acaacgaaaa ttactcttca ttctccgcca gttcgcgcag atactggaaa atctggcgtg
     8341 cggatttcgg cggcttattc ccttctttct ctttcttcgc gttgcggatc agagtacgca
     8401 gttgctgacg atcggcatcg ggccacagat ttaatacctc agcgatggcg tcatcacctt
     8461 gatcgatcag acgatcgcgc aggttttcca gtttatggaa cagcaccacc tgctggttgt
     8521 gacggttttt cagcttatcc agcgcctgac ggatcggttc tacatcgcgc tggcgcagca
     8581 tcttaccgat gagctgcaac tggcggcggc gaccttccat cttaatacgc tgggcgagct
     8641 caatagccgc gcgcagatcc gcatctaacg ggatcttatc cagcgcgttt ttccccagat
     8701 caacaatttc cgcgccaagg cgttttagct cctcggcatc acgtttaatt tcacttttac
     8761 tgacccagat aatttcatcg tcttcgtctt cgatgtcatc accgggaacg tcgtcgagcc
     8821 agtcttcggg ctgcttagtc atctcaggct ccttaaaaaa agaggctaat gttaccagtt
     8881 aagatgcgca ctgaaaaacg gttctctgtt agacttcaga gaaactctct acattatggc
     8941 acttgcaatg aaagtaatct ctcaagttga agcgcagcgc aagattctgg aagaagcagt
     9001 ttccactgcg ctggagttgg cctcaggcaa atcggacggt gcggaagttg ccgtcagcaa
     9061 gaccaccggc attagcgtaa gcacgcgtta tggtgaagtg gagaatgttg aattcaatag
     9121 cgatggcgcg ctggggatca ctgtttatca ccagaaccgc aaaggtagcg catcatccac
     9181 cgatttaagc ccgcaggcca ttgcccgtac tgtacaggcg gcgctggata ttgcccgtta
     9241 tacctcgcca gatccctgtg ccggcgtggc agacaaagag ctgctggcct ttgacgcacc
     9301 agatctcgac ttgttccacc ctgcggaagt ttccccggat gaagccattg aactggcggc
     9361 ccgcgcagaa caggcggcat tgcaggcgga caaacgcatc accaataccg aaggtggcag
     9421 ctttaacagc cactacggtg tcaaagtttt tggcaacagc cacggcatgt tgcagggtta
     9481 ctgctcaacg cgtcattcgc tctccagctg tgtaattgcc gaagaaaatg gcgatatgga
     9541 gcgtgattac gcctacacca ttggtcgtgc gatgagcgat ctgcaaacgc cagagtgggt
     9601 tggggtcgac tgtgctcgcc gtactttatc gcgcctgtca ccgcgtaaac tctccaccat
     9661 gaaagcgccg gtcatttttg ccaatgaagt ggcaaccggg ctttttggcc atctggtggg
     9721 ggcgatagcg ggtggatcgg tttaccgtaa atctaccttc ctgctggatt cgctgggtaa
     9781 acaaattctg ccggactggc tgaccattga agagcatccg catctgctga aagggctggc
     9841 gtcgacgcca ttcgacagcg aaggtgtgcg caccgagcgt cgcgatatta ttaaagatgg
     9901 catcctgact cagtggctgc tgaccagcta ttcggcgcga aaactggggc tgaaaagcac
     9961 cggacatgcg ggcggtattc acaactggcg gattgccgga caaggtctaa gcttcgagca
    10021 gatgctcaaa gagatgggca ccgggctggt ggtgacggaa ttgatgggcc agggcgtgag
    10081 tgcaattacc ggtgattatt cccgtggtgc ggcgggcttc tgggtagaaa acggcgaaat
    10141 tcagtatccg gttagcgaaa tcaccatcgc aggtaattta aaagatatgt ggcgcaatat
    10201 tgtcaccgtc ggtaacgata ttgaaacacg cagtaatata cagtgtggtt ctgtgctgtt
    10261 gccggagatg aaaatcgccg gacagtaaaa aaactggcgc gacctgtcgc gctataaact
    10321 aaaaaattac acaaaatcat tcgcactgca tcgagacgga aagtgaatga gggccgggga
    10381 gcgtactcgc agtacgtgac ccggtcgaat gagcgtagtc gtcgatgagg cagtgtgaat
    10441 gatgaagtgt aaataaaaaa ggaagtgagc aatgcgaaaa agcctgttag ctattcttgc
    10501 agtctcctcg ttggtattca gttcggcgtc gtttgccgct gatcttgaag acaatatgga
    10561 aaccctcaac gacaatttaa aagtggtcga aaaagccgat aacgcggcgc aagtcaaaga
    10621 cgcgttaacg aagatgcgcg ccgcagcgct ggatgcgcaa aaagcaacgc cgccgaagct
    10681 cgaaggcaaa tcaccggaca gcccggagat gaaagacttc cgtcacggtt tcgatattct
    10741 ggtcggtcag attgacgacg cgctgaagct ggcaaatgaa ggtaaagtaa aagaagcgca
    10801 ggctgccgca gagcaactga aaacgacccg caacgcctat caccagaagt atcgttaatt
    10861 cctcatttcc ctgttgcctg cactcaggta acagggaact tatcatcgca aatgatgcac
    10921 cacctgattg ctgctgccgc gccagatcag ggaagggtct tttaaatcct gcacaaattt
    10981 gccgtcgacc agcacgttaa tcaaatcaac aacctgcatt tgcgctgcgt tgagttcgtc
    11041 gagtttatag cctgtccaca cccagatgtc tttacccgga cactcggcgc ggatgcgttg
    11101 taccagtttc agaatatccg gcacgttttg cggatgcagc ggatcgccgc cggagaggga
    11161 aataccctgg cgtttgatac gagtgtcatt cagatcgtta atgatctggt cttccattgc
    11221 tttagtaaat ggctgaccgg aatttacccg ccatgtgctt ttgttatagc aaccggggca
    11281 ttcatgaaca cacccggaga caaacagagt gcaacgagtg ccggggccgt tgacgatgtc
    11341 gacaggatag tactgatgat aattcattat ttcgcctggc ttatcagtcc gcgtgtagac
    11401 tgcatccggc aatcaacgcg ggatgcgacg ctttcacatc acatccggca tttgcttagt
    11461 gcagatcaac ctatctgccc atttcccaaa tgtttaacgc ggcgcttaac ttcttcctgc
    11521 ttgccggcgt taaacggacg tgcatccggg ctacctaaat atccgcacac gcggcgagtt
    11581 accgacacac gggaggcgtc atggttacca catttcgggc aggtgaagcc tttgctggtg
    11641 cactcgaact caccggtaaa gccacactcg tagcactcat caatcggtgt attggtgccg
    11701 taatacggta catgctgata gctgtaatcc cagacatctt ccagcgcctt caggttgtgc
    11761 tgaatgtttg aatactcgcc gtagcaaatg aaaccaccgt tcgccagcgg cgggtaaggc
    11821 gcttcaaagt cgatcttgtc gtacgggttc accttcttct ccacatcgag gtggaaactg
    11881 ttggtgtagt aacctttatc ggtcacgccc ggcaccacgc caaactcagc agtatcgaga
    11941 cggcagaagc ggtcgcacag gttttcactc ggcgtgctgt agagactgaa accataaccc
    12001 gtttcttcct tccactgatc cactgcctga cgcagacgtt caacaatggc gatacctttc
    12061 gcgcgaagct gctcgttgtc gtaaacatgc tcgccgccga acagcgcgtt aatggtttcg
    12121 tggatgccga tgtaacccag cgaaatagac gcacgaccgt ttttgaagat ttcagaaaca
    12181 tcatcgtcag cattgagacg cacgccacaa gcaccttcca tatagaggat cggggccacg
    12241 cgcgctttca cgccttcgag acgagcgata cgggtcatca gcgccttacg tgccagcacc
    12301 agacgttcat ccagcagctt ccagaaggtg gcttcatcgc cttttgcttc cagagcaata
    12361 cgcggcaggt tcaggctgat cacgccgagg ttgttacgac catcgtggat ctgctcgccg
    12421 ttttcatttt cccacacgcc gaggaagctg cggcagccca tcggagtttt aaacgaaccg
    12481 gtgactttca ctacctgatc gtagttcagg atatccggat acatgcgctt gcttgcgcac
    12541 tccagcgcca gctgtttgat gtcgtagttc ggatcgcctt ttttatggtt caggccatcg
    12601 cgaatcgcaa acaccagttt cgggaacacc gcagttttac ggtttttacc gaggcctgcg
    12661 atacggttac gcaggataga ttcctgaatc aggcgcgact cccagctggt gcccagacca
    12721 aaaccaaagg ttacaaacgg cgtctgaccg ttggcggtgt gcagggtgtt tacttcgtac
    12781 tccagcgact ggaaggcatc gtagcactct ttaatggtac gagagttagc gtagccttcg
    12841 gcgtccggga tgttccactc ttctgcggtc ttgcgatgtt tgttgtagct ggcggtgaca
    12901 aacggtgcca gcacttcatc gatacggtta atggtggtgc cgccataaat atggctggcg
    12961 acctgagcaa taatctgcgc agttacagcg gttgccgtag agatcgactt cggcggttca
    13021 atctcggcgt tccccatctt aaagccctgg gtcagcatgc ctttcaggtc gatcaacatg
    13081 cagttgaaca tcgggaagaa cggcgagtaa tcgagatcgt gatagtgaat atcgccacgc
    13141 tcatgtgcct gcaccacgtc acgcggcagc aggtgctgac gtgcatagtg tttagccacg
    13201 atcccggcca gcaggtcgcg ctgggttgga atcaccttgc tgtctttgtt ggcgttttcg
    13261 ttgagtaacg aggcgttggt ctgctcgacc agaccacgga tctcctgatt caggcgaccg
    13321 cgtttttcac gttcaatgtc gcgatcgtga cggtactcga tgtaagcacg agccagttgt
    13381 ttgtatggac ctgacatcag ctggttttca acagcggtct ggatctcatt gatatccacc
    13441 tggttgcggc cctgcatctg ctcgctgaca actgcagcaa cagtggcgca ataatcggca
    13501 tcatcgactt ccgctgcttt agctgcacgc agaatcgctt ctttgatgcg ctctgattta
    13561 aacggcactt tgcagccgtc tcgtttcatc acatgcggtg tcatgatcgc tccatatttt
    13621 taagaacagg ttatccacag aaattgggaa aggcgttttc cggttttttc gtttcgtttt
    13681 ccggtgcttt ccgcaatcgc catccgcttt tccacaacgc cgcacctttt tcgtggggag
    13741 ctgtagtagc aattatagtc gattaataca acatattggg ttgggacgca ttttaaagtc
    13801 tatatatagt gctttgcatc aaggatgttt gaactttttt tgatgtagct caaagtaaaa
    13861 agcagagcgt acggatgacg ggcgctacag cgttatgtaa attttttaat gaatttgctg
    13921 gttgaaaaat aaacaaaaac aacatgctga cagacaaaac ccggaatgac cggcattacc
    13981 ggggcttagg gaagatttac ttctgtaacc accagacagc ctcaaaaggc cgtaaattca
    14041 tggcacaggg ttgtggtgag gcttcttcgt agttatgcat cacaagctgc cagttgccgc
    14101 gcatttgccc tggctgccag ggttggatct cacggctaag gttggcaatg accagcaagg
    14161 tttgcccctt ccattcacgg cgatagcacc acaatacagg gctgtttggc agcagatcct
    14221 ggtaattgcc ccatgtcagg atggcttcct gcttacgcag tgcgattaac ttttggtagg
    14281 tgtaaaacac cgaggaatca tcggccagcg cggcttctac gttgatttgt tgatagttat
    14341 cacccaggcc aatccacggt tcgccagccg taaatccggc attatcgccg ttgctccatt
    14401 gcatgggcgt gcggctgttg tcacgggatt tactggcgag gattgccaat aactcgtcgg
    14461 catcacgacc atcgttgcgc agctcggcaa acatattgag gctctctacg tcgcgatagt
    14521 cagtaatgcg cgtgaaatgc gggttggtca tgccaatctc ttcgccctgg tagatatacg
    14581 gcgttccctg catgccatgc agcaccatcg ccagcatttt tgccgcaggc acgcggtatt
    14641 caccttcatc accaaagcga gaaacaatgc gcggctgatc gtggttacac cagaacaagg
    14701 cattccatgc tacgttgtgc attccttgtt gccagtggcg gaacaatgtt ttcaacgcca
    14761 caaagtcagg tttagccagc gtccattttt caccaccggg ataatcgacc ttcaggtgat
    14821 gaaaattaaa ggtcatcgac aattcactgc ctgtcagtgc cgcgtatcgc tggcaatgct
    14881 caaggctggt ggaggacatt tcacctacgg tcattaaccc gcgtggcgta aacacatcgc
    14941 ggttcatctc gtgcaaaaac tcgtgtgctc gtggcccgtc ggtgtagaag cgacgcccgt
    15001 cgccatccag gtcatcaggg aaacgcgggt ctttggagat cagattcacc acatccaggc
    15061 gcaacccgtc gaccccacga tcggcccaga actcacagac ttttttcagc actgcgcgta
    15121 ccgctggatt ctcccagttg agatccgcct gttctggtgc aaagagatgc aaatagtact
    15181 gttcgctttc cgcatgccag cgccacgcac taccgccaaa ttttgaacgc cagttgttcg
    15241 gtggcgtttc tggttctcca tcgcgccaga tataaaactg gcggtaaggg ctttctttgt
    15301 tcagcgcctc gcgaaaccag gcatgttggg tagaggtatg gttaaacacc atatcgagaa
    15361 tgatacgaat cccgcgcgat tttgcctgcg tcaccagttc gtcaaaatcg tccagcgtgc
    15421 cgtaggtggg atcaatcgcc gtatagttcg ctacgtcgta accgttatcg acctggggag
    15481 agacataaaa gggggttagc cagatggcat caacgcccag tttatgcaga tagtccaggc
    15541 gttggataac gccacgtaaa tcgccggtac cgctacccgt ggtgtcctga aaactctttg
    15601 gataaatctg gtagataacg ccgttttgcc accagtgggg aggattagtc attacgttat
    15661 tcctgcaaat gcgagggggc gcaattgcgc cccgaagaaa attaaacaat gtccagcgtg
    15721 cccaggcggt atttccgctg atagataaac gaggtgagta caatcgggat gatgatggcg
    15781 atagccattg ccagcgcaaa cacctgccag tagttcggtt gaatcgagag aattcccggc
    15841 aggccgccta cgccgatgcc attcgccata acgccgttca ggccgcatag caatcctgcc
    15901 agaccagaac caatcatcgc gcacagcatc gggaagcgat atttcaggtt gatgccgtac
    15961 attgcaggct cagtgacccc aagccaggcg gagatcgccg caggcacgga gatctcgcgt
    16021 tcattgtgct tgcggctgga aatgataatg ccgatcacgg cggagccctg agcgatattc
    16081 gacagcgcaa tcagtggcca cactggcgta ccacccatgc tttgaatcat ctgcaaatca
    16141 atagcaagcg tggtctggtg taccccggtg atcaccagcg gggcgtacag gaagccaaac
    16201 aatgccgcgc caatcggagc aaagctgccg gtcatcaggt gacgtaccgc aaaggcaacg
    16261 ccatcgccaa tcatgcgacc aaacggacca atcagcgcat gggcgaggaa caccgcgagg
    16321 atcagcgaac agacgggcac caccaccaga tagaggtaat ctggcacgat gcgtttaagg
    16381 cgagtttcaa taacgcccag cgccagcccg gctaacagtg ccgggatcac ctgcgcctga
    16441 tagcccactt tggcgatgct gaacatgcca aagtcccaca cttccggcag ctgctgcccg
    16501 agcagataag cgttcatcag ttggggggaa accagtgtca cgccaagcac gataccaagg
    16561 atcggtgtgc cgcccatttt tttcaccgct gaccagcaaa tcccgaccgg caggtagaag
    16621 aagatcgctt caccgatcaa ccacagaaaa tcgtagatcg tttgcaggga agggtacatt
    16681 tgcgccagcg tttgaccgtt gctcatgggc aaatcgccga tcacattgcg aaaaccgagg
    16741 atcaaaccgc cgctaatcaa cgcgggcagc aacgggaaga aggtctctgc gaaatgagag
    16801 atcaactgct catgccattt catattctgc cgggcggctt tttttacctg ctctttatca
    16861 acctgcgcct gtccggttga cgctatcagt gcttgatagt agtcacccac gttggtgcca
    16921 atcaccacct gaaattgcct ggcattggtg aaacagcctt tcaccatggg gagttgctca
    16981 atttctttcg gtctggcatt ggccggttgg ttgaggacaa agcgtaggcg agtaatacag
    17041 tggctcaccg tcgcaatatt gccgcgcccg ccgaccagtt caatcaaccg atcgatatcc
    17101 gtttggttta ttttgctcat cataaagccc catggcagat gacatttttg gttggctgca
    17161 gaatatattg cgcggaaaaa tttaaaaacg ggaacgttcc cgaaacgcag cgaagatcac
    17221 aatttatcgt tcaggaaacg atctggacag ggtggcgggg atgatgattt gttgcggttc
    17281 gctgcgcccg gttacctgtg cgatcaactg gcaggccgcc tggcgtccag cttcggcgta
    17341 accgggatct acggtgacga tctccggatg gaggaatttc attaacggcg tattaccgac
    17401 gctcgccagt tgcaaggtgt cgatgcgttg ctcttgcagg tatttacttg cgccaagtgc
    17461 cagcgtgtcg gttgcgcaca gtaaggcggt agtttcaggc gtaatcactt ttgctacgtt
    17521 ctcatagcct tgcttcatag caaggcctgg cagagcggca acgggatgca gtttatgcgc
    17581 tttgcagaac gccaggtagg cttcgtgacg tcgcttaccg gtcgtcacgt cactgtgcgg
    17641 cacgccgaga taactgatat tacgatgccc ctggtcatac agccgttgca tcaggatctt
    17701 gattgcccct tcgtcgtcat aacagaccga agcaaagcct tttgcgtcac gcgccagcag
    17761 aaccagcgat gactgccagt gggctaacat ttcttctgtt atgccggtaa aaccgaacag
    17821 cactacgccg tcgatattac gccgtttcag cacccccaaa tgttcggcaa ctaattgcgg
    17881 ggaaaactga ctttccatca tgattgggtc gtaaccttgt tcatagaact ctggcagcat
    17941 ggtttgaacg gcgagatttt ctgacaacga atccaggcgg gtaacaatga tggcgaccac
    18001 tttatcgctt tgcccacgca tagcgcgcgc agagcgggaa ggggaaaatc catgctgatt
    18061 catcactgct tcaacacgct cgcgggtgcg ctggctcacg ccgctttcgt tattcagcac
    18121 ccgggaaact gtagatttcc ccacgccgct taagcgcgcg atgtctttga tggtcagccg
    18181 attttgcatc ctgttgtcct gtaacgtgtt gtttaattat ttgagcctaa ctttacccgt
    18241 gcattcagca atgggtaaag tctggtttat cgttggttta gttgccagca ggtattatat
    18301 cgccatagat gctacgaata ttattggatt ctccttatta tttgcggcgc ttttttcact
    18361 taccggaggt tatatggaac ctgatcccac gcctctccct cgacggagat taaaactttt
    18421 ccggtaagcc cgtcttttca cggcgttacc ggatgcgtaa ggccgtgacg ttttaacgtc
    18481 cctgctcagc tttattacct tcaggtaagg cttcgccacg cctgaagaca tttctgtact
    18541 gtttcagaca gtgcggaggg actccttatg tttaaagaaa tttttacccg gctcattcgc
    18601 catttacctt cccgtctggt tcatcgtgat ccattgcctg gcgcgcagca gacagtgaat
    18661 acggtggtcc cgccgtcctt aagtgcgcat tgcctgaaaa tggcggtgat gcccgaagaa
    18721 gaattgtgga aaacgttcga cacccatccg gaagggttaa atcaggcgga agtggaatct
    18781 gcccgcgaac aacatggtga aaataaatta cccgcacaac aactgtcgcc gtggtgggta
    18841 catttatggg tctgctatcg caaccccttt aatattttac tcaccattct cggcgctatt
    18901 tcttacgcca cggaagattt atttgctgcg ggcgttatcg cgctaatggt cgctatttct
    18961 acgttgctga actttattca ggaagcacgt tccactaaag cggcagatgc cctgaaagcg
    19021 atggtcagca ataccgcgac ggtgctgcgc gtaattaacg acaaaggcga aaatggctgg
    19081 ctggagatcc cgatcgacca gctggtgccc ggcgatatta taaaactggc ggcgggggat
    19141 atgatcccgg cagatttacg tatcttgcaa gcgcgggatc tgttcgtcgc tcaggcgtcg
    19201 ttaaccggtg agtctctgcc cgtggaaaaa gccgctacca ctcgccagcc ggagcacagc
    19261 aatccgctgg agtgcgacac gctgtgtttt atgggcacca ccgtggtgag cggcacggca
    19321 caagcaatgg tgattgctac aggtgccaat acctggtttg gtcaactggc ggggcgtgtt
    19381 agtgagcagg aaagcgagcc gaatgccttt cagcaaggga tcagccgcgt cagtatgctg
    19441 ctgattcgct ttatgctggt gatggcaccg gtggtgctgt taatcaatgg ctacaccaaa
    19501 ggcgactggt gggaagcggc gctgtttgcg ctttcggtcg cggtaggcct aacgccggaa
    19561 atgttgccga tgattgttac ctcgacgctg gcgcgcggcg cagtaaagct gtcgaaacag
    19621 aaagtgatcg tcaaacatct ggatgctatt cagaactttg gcgcaatgga tattctgtgc
    19681 actgataaaa ccggcaccct gacgcaggat aaaattgtgc tggagaatca taccgatatc
    19741 tccggtaaaa ccagcgaacg cgtgctgcat agcgcgtggt tgaacagtca ttatcagacc
    19801 ggacttaaaa acctgctcga tacagcggtg ctcgaaggta cggatgaaga gtcagcgcgc
    19861 tcgctggcca gtcgttggca aaaaatcgat gagattccgt ttgatttcga gcgtcgccgg
    19921 atgtcggtgg tagtggcaga aaataccgag caccatcagc tggtttgcaa aggtgcattg
    19981 caggaaatcc tcaatgtgtg ttcgcaggtg cgtcacaatg gcgagattgt gccgctcgat
    20041 gacatcatgc tgcgtaagat taagcgggtt actgatacgc tgaatcgtca ggggctgcgc
    20101 gtggttgcgg tggcgacgaa atacctgcca gcgcgtgaag gagattacca gcgggcggat
    20161 gaatccgacc tgatcctcga aggatatatt gcttttcttg atccgccaaa agagacaacc
    20221 gctccggcac tgaaggcatt aaaagcgagt ggaattaccg taaaaatcct tactggcgac
    20281 agtgagttag ttgcagcgaa agtgtgccat gaagtggggc tggacgcggg agaggtggtc
    20341 attggtagtg atattgaaac gctatctgac gacgaactgg caaatcttgc acagcgcacc
    20401 acgctgtttg cccgcctgac gccgatgcat aaagaacgca ttgtgacctt actgaagcgc
    20461 gaagggcatg tggttggctt tatgggcgat ggtattaatg atgcgcccgc cttacgcgcg
    20521 gcggatatcg gcatttctgt ggacggcgcg gtagatattg cccgtgaagc ggctgatatc
    20581 atcctgctgg aaaaaagcct gatggtgctg gaagaggggg ttattgaggg acgtcgcact
    20641 ttcgccaaca tgctgaaata catcaaaatg acggcgagtt ctaactttgg taatgtgttc
    20701 agcgtgctgg tggcgagtgc tttcttgccc ttcctgccga tgttgccgtt acacttgctt
    20761 attcagaacc tgctgtacga tgtgtcacag gtggcgatcc cgtttgataa cgtcgacgac
    20821 gagcaaattc aaaagccgca gcgttggaat ccggcggatc tggggcgctt tatgatcttc
    20881 ttcggaccga tcagctcgat cttcgatatt ctgacgtttt gcctgatgtg gtgggtattc
    20941 catgccaaca cgccggaaac gcaaacgctg ttccagtcgg gatggtttgt ggtgggctta
    21001 ctgtcgcaaa cgctgattgt gcatatgatc cgcacccgcc gtgtgccgtt tattcagagc
    21061 tgtgcatcgt ggccgttaat gatcatgacc gtgatcgtga tgattgtcgg gatcgcattg
    21121 ccgttttcac cgctggccag ttatctgcaa ttacaggcgt tgccgttaag ctatttcccg
    21181 tggctggttg cgattctggc agggtatatg acgttaaccc agttggtgaa aggattttat
    21241 agccgtcgtt acggctggca gtaaagaata aactaggcat ggtggcccgg attcagattg
    21301 cagacaaagt gagttttgtt catgccggat gcggtgtaaa cgcctaatcc ggcctacaaa
    21361 ttcaatcaat tgcaggaatc acgtaggcct gataagcgta gcgcatcagg cgattttgcg
    21421 tttgtcatca gacttaatcc gggcatgata gcccggattt ccatcaagat tagcgacgaa
    21481 tagcgatcgc ttcgatctca atctttacgt ctttcggcag acgggcaact tcaacgcaag
    21541 aacgtgccgg gaaggtggcg ttgtgttcgg tgaagaaggc ttcgtaagtg gcgtttacgg
    21601 ttgcgaagtc gttcagatct tttacaaaca cggtagtttt aacgatgtcg cccactttca
    21661 gaccagcggc ttcgacgatc gcttttacgt tatccagcga ctgacgtgcc tgtgcagcga
    21721 cgtctgccgg tacttcgccc gttttcggat ttaccgggat ctgaccggag gtgatgatca
    21781 tatttcccag atcaacgccc tgtacgtaag gaccgatagc tgctggtgca ttttccgtcg
    21841 cgatagtttt gctcatgatt tctcctttat tacagcggta aaaagtctgg ctcattatag
    21901 ggagccagac ttttattacc aaccgcaatt aattggccag caccacatta tgggaaaact
    21961 ctttttcaca gtatttgcat ttgagcgcga tgtcattggc gcgttttcgc acggcaaagc
    22021 tggatgaaac cggttcggca tggctgatac agttgctgtt cgggcagacc aacacattgt
    22081 cgatgcgctc tggcagactg gggcgcgatt tacccaccac ttcatagttg tcgatacggt
    22141 taaccgtggc ttgcggcgca tacaatgcca gttgatctac ctgatcttca ctcaaaaagg
    22201 tattttcgat tttgatcaga tctttgcggc ccatctcgcc agaaggcagg ttcagaccaa
    22261 tggtgatgcg ctgatccgtt tcggtcagct tgaacagact caacagctta aaaccgatct
    22321 gggcggggat atggtcaatt accgtgccgc gtttaatagc ttcaacctgc aatttattat
    22381 cgtgtgtcat ctctatttcc ccttacagta ccagatcgcg attcagaacc agtgccagta
    22441 acgcctggcg agcgaaaatc ccgttgcctg cctgctggaa gtaccaggcg tgtggcgttt
    22501 tatcaacatc cgtcgcaatt tcatcaacac gcggcagcgg atgcagtact ttcatattgg
    22561 ctttggcgtt gtggagatcg ctggcgcgaa gaacaaactg cgctttcacg ttggcgtatt
    22621 cggatgggtc caggcgctct ttttgcacgc gggtcatata caggatgtct acttccgcca
    22681 tcacttcttc aatagagctg tgcagactcc atgcgatccc tttttcatcg agcatatcca
    22741 gaatgtattg cggcattgcc agcgcgtccg gcgcgatgaa gtaaaaacgg ttgccgtcga
    22801 acttcgctaa cgcctgagtc agggagtgaa cggtgcggcc atatttcagg tcaccaacca
    22861 ttgcgacgtg gagattgtcc agacgcccct gggtttcctg aatagtgaat aagtccagca
    22921 aggtttgcgt cggatgttgg ttggagccat caccggcatt cagtaccggt acattgccgg
    22981 aaaactcggt ggccaggcgc gccgcacctt cctgcggatg acgcatcact atcgcatcga
    23041 cgtaagtgct gataaccgaa atggtatcgg ccagcgtttc gcccttttta cccagtgatg
    23101 tattggcgct gtcggagaag cccaccacgc tggcacccag gcggtgcatg gaagtttcga
    23161 aagagaggcg ggtacgggta gaggcttcga agaagcagct ggcaatgact ttgtgcttca
    23221 acagctctgg ttgcgggttt gctttcagtt ttgccgctgt tgccagcacc agattaaggt
    23281 catcgcgact aaggtcgttt atggaaatga tatgtttctg atatagcgga ttagccatct
    23341 tttatctcct gacgcctggg caaaaaaaag cccctcgatt gaggggctgg gaatgggtga
    23401 tcaacgggaa gaaaaacggc aggccagcgt cttttttcag acgcggtaag acaaaatgtc
    23461 gaacacactg aaccatacat cctcccggca aattgtccgg cattatactc atcgtcagaa
    23521 gcggtgcaag caattgatgc aatattttgt cagcgcaaac ggtttatttg aatttaaagt
    23581 taaaatatat gcatttttat ttactgtgat tctgggggga actgtcgttc ggtatcaata
    23641 attgcagaca tttcctgctt ttttcttttt cactttcacg caatcagata tgcattttat
    23701 tccattcatc cgacttatag ggcggagtgt gaaaacgaac ggctagcact attgcttaga
    23761 gctcagggat gcgcgctatc actttaattt caaaatcaaa gcctgccagc catgtaacgc
    23821 ccaccgccgt ccaggttgga taagatggcg cgctaaatat ttcatttttc accgtcatga
    23881 tgtcttcaaa ttgtttttct ggatcggtat gaaagctcgt aacatcaatg atatcgtcaa
    23941 aagtgcatcc cgcagctgcc agggtcgcat gcaaattatc aaaggccagt ctgacttgtt
    24001 gctgaaaatc gggttctggt gttccgtcct ctcgacttcc tacttgcccg gaaacaaaca
    24061 gcaaatcgcc ggaacgaata gccgcagaat aacgatgctc agcatacagt gaatgtcgtc
    24121 cagcagggaa aacagcggtt ctttctacca tttggttatc ctcaagattt acgacatgaa
    24181 cagaagattt atatttacca ggagccgctt ttagcggacg acgtgagtaa acaaaaccca
    24241 gacatcatgg gaaatggctg ggcttaattg agcgtagtcg gttatgcgcc aaacgcgcca
    24301 tcaatggtat gcatcgcgcc ggtaacaaaa ctggcttctg gccctgctaa ccatgcgacc
    24361 ataccagcga cctcttccgg ttgcccatgt cttttgatgg ccataaaact atgcgacata
    24421 tcgcgcattg gcccgttggc gggattagcg taggtatcaa ttggccctgg ctggacgacg
    24481 ttgatggtga tcccacgcgg tccgaaatca cgggccagcc cgcgcgccat gccttgcagg
    24541 gcagatttac tggcggcata agcagccatg cctgcaacag gcatacgatc gccattcacg
    24601 gagccgatga ttaagatgcg tccgccttcg ggcatctgcc gggcggcttc aacagaggca
    24661 tggtaaggag catgaatatt gattttgaaa aggcgatcaa tatcgtcggc atttaattcc
    24721 agggcatcgc caaagacgcc aatacctgca tttaccacca ggatatccaa tgcgccgctc
    24781 ttacgaacga catcaataac agcgtctttg tcagcactat ctgtgaatac tgctgtcgct
    24841 ccagtctctt gtgccaggcg ttcagcggca tctttcgacc ccgcataggt gaatcgtaca
    24901 ttggccccat cggtgacgaa acgacgtacg atagcggcac cgattccacg gctaccaccg
    24961 aggatgagaa ctgtcttacc tgtaaaagcg cccataagga ctccttgatt tattatgtaa
    25021 catgcattac aaaactgttt taactttctg tcaacaagtt ttgtaatggt cactaaaaaa
    25081 caatctcgcg ttccaggtcg tcccagacgt ttcgctcctg agcaggcagt ctctgcggca
    25141 aaagtgcttt ttcaccaaaa aggtttcgat gctgtcagtg ttgctgaagt tactgattat
    25201 cttggtatta accccccgag cctctacgcg gcttttggca ataaggctgg gttatttagc
    25261 cgtgtactca atgaatatgt cggtacggaa gctattccgc ttgccgatat tcttcgtgat
    25321 gatcgtccgg taggggagtg cctggctgag gtattaaaag aagcggcgcg ccgatatagc
    25381 caaaacggcg gctgcgctgg ctgtatggtt cttgaaggca ttcatagtca tgatccacaa
    25441 gcgcgtgata ttgccgttca atattatcac gccgcagaaa cgaccattta tgactatatc
    25501 gccaggcggc atccacaaag cgcacaatgt gtgactgatt ttatgagtac cgtgatgtca
    25561 gggctttctg cgaaggcacg ggaggggcac tccatcgaac aactctgtgc aacagctgca
    25621 ctggcggggg aagcgataaa aactattctc aaggagtgat gcgggccttg acccgaaagg
    25681 cgggaacgcg cctgccgata agttgtgata agacaataat tcacgcgtta aggctagcgg
    25741 aattgatcat cttttcgtat aacgatggaa atgaaacgtt gttttaatta aggagtggaa
    25801 aagatgatca tcggaaatat tcataatctt cagccgtggc taccccagga gttacgccag
    25861 gcgattgagc atatcaaagc acacgttacg gcagaaacgc caaagggcaa gcacgatatc
    25921 gaaggcaatc gtctgtttta tcttatctcg gaagatatga ccgagccgca cgaagctcgc
    25981 cgtgcggagt accatgcccg ctatctcgac attcagattg tgttaaaagg tcaggaaggc
    26041 atgaccttca gcacgcaacc tgcaggcacc ccggataccg actggttagc tgataaagac
    26101 atcgcatttt tgccggaagg cgttgatgag aaaacagtta tccttaatga aggtgatttt
    26161 gttgtgtttt atccggggga agtgcataaa ccgctgtgcg cagtgggcgc gccagcccgg
    26221 gttcgcaaag cagtagtgaa gatgctgatg gcgtgatgac ttttcgccgt aaataactcc
    26281 aggtttacgg cgagtttgtg aaaagagcgt tttttgatat ttttttgtga gtaaaatttg
    26341 taatgcttag acgttcttat taactcaagg agttcgtcat gagcaaaata tcagatttga
    26401 attattctca acacattaca ttagccgaca attttaaaca aaaaagtgaa gttttaaata
    26461 cctggcgtgt tggaatgaat aattttgccc gtaatgccga ggggcaggat aacacaagaa
    26521 atatccttga tcctaagaca tttttggaat ttttggtaaa aatatttact ctaggttatg
    26581 tggattttag caaacgctcc aacgaagcgg gaagaaatat gatggctcat attgagtcct
    26641 catcttatat caaaaataat gatggcagtg agataataaa gtttgttatg aataatcctg
    26701 aaggggaacg agcggattta tctaaggtgg aaatagaaat cacactttcc actttcacta
    26761 ctatggggac tcgtcaagga catacggcta ttatatttca acaacctgat ggttcgacta
    26821 atcgttatga aggaaagtcc tttgaaagaa aagatgagag ttcattacac ctgattacta
    26881 acaagattct ggcatgttac caacgcgaag ctaacaagga aatagctcgt ctattaaata
    26941 tttcacagga gttaaataat tctcaggact tgaataactc gcaggtgagt tgtaaagatt
    27001 cagttgattc tacgattacg gatttattag aaaaaccatt gaataatgca ttattagcaa
    27061 taaggaaaga acatctgcta ttaatgcctt atgtatgtaa tgaatcgatt tcatacttgc
    27121 ttggtgaaaa agggatactg aaagaaatag atgaccttaa cgcagtaaat aattatcttc
    27181 ttaataataa gaaagctact gacaatgaaa ttaatgacat caaagttaac ctgtctcata
    27241 ttcttattga ttccttagat gatgcaaaag ttaaccttac accggtcatc gattctggat
    27301 gactttttta aaatccccat atattaatga tgtaagaata ctggattggt gttttaataa
    27361 aagaatgcaa tattttggtg attctgaaaa gataaagtat gcatgctccg tgataaatca
    27421 tattgatttt agccgcgatc aatctaaaga ttttagctgc gatcaatcta aaattaaaat
    27481 agctgagacc ttatttttca atctcgataa agaaccctat aaaaa
//
LOCUS       DADLFA010000036        27434 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000036 DADLFA010000000
VERSION     DADLFA010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27434
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.036"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            454..3639
                     /gene="rne"
                     /locus_tag="JQH65_001311"
     CDS             454..3639
                     /gene="rne"
                     /locus_tag="JQH65_001311"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HAY6138784.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPAAPA
                     QPGLLSRFFGALKALFSGSEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPHHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(3836..4779)
                     /gene="flgL"
                     /locus_tag="JQH65_001312"
                     /pseudo
     CDS             complement(3836..4779)
                     /gene="flgL"
                     /locus_tag="JQH65_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-filament junction protein FlgL"
     gene            complement(4791..6434)
                     /gene="flgK"
                     /locus_tag="JQH65_001313"
     CDS             complement(4791..6434)
                     /gene="flgK"
                     /locus_tag="JQH65_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HAY6138785.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNKGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEKDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSATQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(6500..7441)
                     /gene="flgJ"
                     /locus_tag="JQH65_001314"
                     /pseudo
     CDS             complement(6500..7441)
                     /gene="flgJ"
                     /locus_tag="JQH65_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
     gene            complement(7441..8538)
                     /locus_tag="JQH65_001315"
     CDS             complement(7441..8538)
                     /locus_tag="JQH65_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HAY6138786.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSDGSLQSVRSSARLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(8550..9248)
                     /gene="flgH"
                     /locus_tag="JQH65_001316"
     CDS             complement(8550..9248)
                     /gene="flgH"
                     /locus_tag="JQH65_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HAY6138787.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVVDARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(9301..10083)
                     /gene="flgG"
                     /locus_tag="JQH65_001317"
     CDS             complement(9301..10083)
                     /gene="flgG"
                     /locus_tag="JQH65_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HAY6138788.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMQQKLTQL"
     gene            complement(10221..10976)
                     /locus_tag="JQH65_001318"
     CDS             complement(10221..10976)
                     /locus_tag="JQH65_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
                     /protein_id="HAY6138789.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKIDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHQVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(10996..12924)
                     /gene="flgD"
                     /locus_tag="JQH65_001319"
                     /pseudo
     CDS             complement(10996..12924)
                     /gene="flgD"
                     /locus_tag="JQH65_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706995.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
     gene            complement(12936..13340)
                     /gene="flgC"
                     /locus_tag="JQH65_001320"
     CDS             complement(12936..13340)
                     /gene="flgC"
                     /locus_tag="JQH65_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HAY6138790.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(13344..13673)
                     /gene="flgB"
                     /locus_tag="JQH65_001321"
     CDS             complement(13344..13673)
                     /gene="flgB"
                     /locus_tag="JQH65_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HAY6138791.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTLGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYQMSLSALSGQIKGMM
                     NVLQSGN"
     gene            13829..14488
                     /gene="flgA"
                     /locus_tag="JQH65_001322"
     CDS             13829..14488
                     /gene="flgA"
                     /locus_tag="JQH65_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HAY6138792.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLHTFFSAQLVGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTHFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARMRMVSGLVVSGVVDADGNILIN
                     L"
     gene            14564..14875
                     /gene="flgM"
                     /locus_tag="JQH65_001323"
     CDS             14564..14875
                     /gene="flgM"
                     /locus_tag="JQH65_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HAY6138793.1"
                     /translation="MSIDRTSPLNPVSTVQPRETTDAPVTNTRAAKTTDSTSTSTSTS
                     TSVTLSDAQAKLMQPGSSDINLERVEALKLAIRNGELKMDIGKIADALINEAQQDLQS
                     N"
     gene            14880..15296
                     /gene="flgN"
                     /locus_tag="JQH65_001324"
     CDS             14880..15296
                     /gene="flgN"
                     /locus_tag="JQH65_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HAY6138794.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(15336..16871)
                     /gene="murJ"
                     /locus_tag="JQH65_001325"
     CDS             complement(15336..16871)
                     /gene="murJ"
                     /locus_tag="JQH65_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HAY6138795.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(17038..17904)
                     /locus_tag="JQH65_001326"
     CDS             complement(17038..17904)
                     /locus_tag="JQH65_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAY6138796.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGE"
     gene            complement(17906..18553)
                     /locus_tag="JQH65_001327"
     CDS             complement(17906..18553)
                     /locus_tag="JQH65_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HAY6138797.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAELESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(18564..19148)
                     /gene="rimJ"
                     /locus_tag="JQH65_001328"
     CDS             complement(18564..19148)
                     /gene="rimJ"
                     /locus_tag="JQH65_001328"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HAY6138798.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            19384..20586
                     /gene="mdtH"
                     /locus_tag="JQH65_001329"
     CDS             19384..20586
                     /gene="mdtH"
                     /locus_tag="JQH65_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HAY6138799.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHRLM
                     AGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSYMG
                     FSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKRAA
                     RRLLERDA"
     gene            20650..21297
                     /gene="grxB"
                     /locus_tag="JQH65_001330"
     CDS             20650..21297
                     /gene="grxB"
                     /locus_tag="JQH65_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HAY6138800.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            21431..21991
                     /locus_tag="JQH65_001331"
     CDS             21431..21991
                     /locus_tag="JQH65_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY6138801.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            22097..23143
                     /gene="pyrC"
                     /locus_tag="JQH65_001332"
     CDS             22097..23143
                     /gene="pyrC"
                     /locus_tag="JQH65_001332"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAY6138802.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVMPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHNFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREAHFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            23217..23462
                     /gene="dinI"
                     /locus_tag="JQH65_001333"
     CDS             23217..23462
                     /gene="dinI"
                     /locus_tag="JQH65_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HAY6138803.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGVTKEDKQRISEILQETWESADDWFVSE"
     gene            23752..24006
                     /gene="bssS"
                     /locus_tag="JQH65_001334"
     CDS             23752..24006
                     /gene="bssS"
                     /locus_tag="JQH65_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HAY6138804.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            24121..25239
                     /gene="solA"
                     /locus_tag="JQH65_001335"
     CDS             24121..25239
                     /gene="solA"
                     /locus_tag="JQH65_001335"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HAY6138805.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            25287..25400
                     /locus_tag="JQH65_001336"
     CDS             25287..25400
                     /locus_tag="JQH65_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2770 domain-containing protein"
                     /protein_id="HAY6138806.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            25661..26227
                     /locus_tag="JQH65_001337"
     CDS             25661..26227
                     /locus_tag="JQH65_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HAY6138807.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFTLGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            26231..26806
                     /locus_tag="JQH65_001338"
     CDS             26231..26806
                     /locus_tag="JQH65_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HAY6138808.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(26848..>27434)
                     /locus_tag="JQH65_001339"
     CDS             complement(26848..>27434)
                     /locus_tag="JQH65_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAY6138809.1"
                     /translation="NHYEYEVGHFENAFEIPADTFREQLPKAVEMMQAHKDKKIVMYC
                     TGGIRCEKASAWMKHNGFNKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGER
                     ISDEIIAHCHQCGAPCDSHTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPE
                     EQRRRRAGRENGNKIFNKSRGRLNTTLGIPDPTE"
BASE COUNT         6580 a         7302 c         6911 g         6641 t
ORIGIN      
        1 gggatgcgtt catataaaaa ctgtgagtaa gcgggtgata aatggtaaaa gtcatcttgc
       61 tataacaagg cttgcagtgg aataatgaag ccgtttccgt gtccatcctt gttaaaacaa
      121 gaaattttac ggaataaccc attttgcccg accgatcatc cacgcagcaa tggcgtaaga
      181 cgtattgatc tttcaggcag ttagcgggct gcgggttgca gtccttaccg gtagatggaa
      241 atatttctgg agagtaatac ccagtctgtt tctttgataa ttgcgctgtt tttccgcatg
      301 aaaaacgggc aaccgacact ctgcgcctct ttgagctgac gataaccgtg aggttggcga
      361 cgcgactaga cacgaggcca tcggttcaca cccggaaagg cgttactttg cccgcagctt
      421 agtcgtcaat gtaagaataa tgagtaagtt acgatgaaaa gaatgttaat caacgcaact
      481 cagcaggaag agttgcgcgt tgcccttgta gatgggcagc gtctgtatga cctggatatc
      541 gaaagtccag ggcacgagca gaaaaaggca aacatctaca aaggtaaaat cacccgcatt
      601 gaaccgagtc tggaagctgc ttttgttgat tacggcgctg aacgtcacgg tttcctccca
      661 ctaaaagaaa ttgcccgcga atatttccct gctaactaca gtgctcatgg tcgtcccaac
      721 attaaagatg tgttgcgtga aggtcaggaa gtcattgttc agatcgataa agaagagcgc
      781 ggcaacaaag gcgcggcatt aaccaccttt atcagtctgg cgggtagcta tctggttctg
      841 atgccgaaca acccgcgcgc gggtggcatt tctcgccgta tcgaaggcga cgaccgtacc
      901 gaattaaaag aagcactggc aagccttgaa ctgccggaag gcatggggct tatcgtgcgc
      961 accgctggcg tcggcaaatc tgctgaggcg ctgcaatggg atttaagctt ccgtctgaaa
     1021 cactgggaag ccatcaaaaa agccgctgaa agccgcccgg ccccgttcct gattcatcag
     1081 gagagcaacg taatcgttcg cgctttccgc gattacttac gccaggacat cggcgaaatc
     1141 cttatcgata acccgaaagt gctcgaactg gcacgtcagc atatcgctgc attaggtcgc
     1201 ccggatttca gcagcaaaat caaactgtac accggcgaga tcccgctgtt cagccactac
     1261 cagatcgagt cgcagatcga gtccgccttc cagcgtgaag tccgtctgcc gtccggtggt
     1321 tcgattgtta tcgacagtac cgaagcgtta actgccatcg acatcaactc cgcacgcgcg
     1381 acccgcggcg gcgatatcga agaaaccgcg tttaacacca acctcgaagc tgccgatgag
     1441 attgctcgtc agctgcgcct gcgtgacctc ggcggcctga ttgttatcga cttcatcgac
     1501 atgacgcctg tacgccacca gcgtgcggta gaaaaccgtc tgcgtgaagc ggtgcgtcag
     1561 gaccgtgcgc gtattcaaat cagccatatt tctcgctttg gcctgctgga aatgtcccgt
     1621 cagcgcctga gcccatcact gggtgaatcc agtcatcacg tttgtccgcg ttgttctggt
     1681 actggcaccg tgcgtgacaa cgaatcgctg tcgctctcta ttctgcgtct gatcgaagaa
     1741 gaagcgctga aagagaacac ccaggaagtt cacgccattg ttcctgtacc aatcgcttct
     1801 tacctgctga atgaaaaacg ttctgcggta aatgccattg aaactcgtca ggacggtgtg
     1861 cgctgcgtga ttgtgccaaa cgatcagatg gaaaccccgc actaccacgt gctgcgcgtg
     1921 cgtaaagggg aagaaacccc aaccttaagc tacatgctgc cgaagctgca tgaagaagcg
     1981 atggcgctgc cgtctgaaga agagttcgct gaacgtaagc gtccggaaca acctgcgctg
     2041 gcaacctttg ccatgccgga tgtgccgcca gcgccaacgc cagctgaacc tgccgcgcct
     2101 gttgtagctc cagcaccgaa agctgcaccg gcaacaccag cagctcctgc acaacctggg
     2161 ctgttgagcc gcttcttcgg cgcactgaaa gcgctgttca gcggtagtga agaaaccaaa
     2221 ccgaccgagc aaccagcacc gaaagcagaa gcgaaaccgg aacgtcaaca ggatcgtcgc
     2281 aagcctcgtc agaacaaccg ccgtgaccgt aatgagcgcc gcgacacccg tagtgaacgt
     2341 actgaaggca gcgataatcg cgaagaaaac cgtcgtaatc gtcgccaggc acagcagcag
     2401 actgccgaga cgcgtgagag ccgtcagcaa gctgaggtaa cggaaaaagc acgtaccacc
     2461 gacgagcagc aagcgccgcg tcgtgaacgt agccgccgcc gtaacgatga taaacgtcag
     2521 gcgcaacaag aagcgaaggc gctgaatgtt gaagagcaat ctgttcagga aaccgaacag
     2581 gaagaacgtg tacgtccggt tcagccgcgt cgtaaacagc gtcagctcaa tcagaaagtg
     2641 cgttacgagc aaagcgtagc cgaagaagcg gtagtcgcac cggtggttga agaaactgcc
     2701 gctgccgaac caattgttca ggaagctcca gctccacgca cagaactggt gaaagtcccg
     2761 ctgccagtcg tagcgcaaac tgcaccagaa cagcaagaag agaacaatgc tgataaccgt
     2821 gacaacggtg gcatgccgcg tcgttctcgc cgctcgcctc gtcacctgcg cgtgagtggt
     2881 cagcgtcgtc gtcgctatcg tgacgagcgt tatccaaccc agtcgccaat gccgttgacc
     2941 gtagcgtgcg cgtctccgga actggcctct ggcaaagtct ggatccgcta tccaattgta
     3001 cgtccgcaag atgtacaagt tgaagagcag cgcgaacagg aagaagtaca agtgcagccg
     3061 atggtgactg aggtccctgt cgccgccgct gtcgaaccgg ttgttagcgc gccagttgtt
     3121 gaagaaatgg ctgaagtcgt agaagccccc gtaccggttg ccgaaccgca accggaagtg
     3181 gttgaaacga cgcatcctga agtgattgcc gccgcggtaa ctgaacagcc gcaggtgatt
     3241 accgagtctg atgttgccgt agcccaggaa gttgcagaac acgcagaacc ggtggttgaa
     3301 ccgcaggaag agacggcaga tattgaagaa gttgccgaaa ctgctgaggt tgtggttgca
     3361 gaacctgaag ttgttgctca acctgccgcg ccagtcgtcg ctgaagtcgc agcagaagtt
     3421 gaaacggtag ccgcggttga accagagatc accgttgagc ataaccacgc taccgcgcca
     3481 atgacgcgcg ctccggcacc ggaatatgtt ccggaggcac cgcatcacag tgactggcag
     3541 cgccctactt ttgccttcga aggtaaaggt gccgcaggtg gtcatacggc aacgcatcat
     3601 gcctctgccg ctcctgcgcg tccgcaacct gttgagtaat aattagctca aagtaatcaa
     3661 gccctggtaa ctgccagggc ttttttattt catgtttgaa tcaacatatt aaaattcatc
     3721 aaatggcatc cttgctaacc aacaatgcaa aataggcaaa aaaaatgccc gccccggcga
     3781 tccctgagcg ggcagacaaa acatacccag tttcatgata tgttttaaag cgagcttatt
     3841 tgttgagctg gaagagcgac aatccctgca tatcggtaaa tgctttatac gatgcctgca
     3901 atgccgtttg ctgcatgatg taagatgaaa tagttgcatt ccagtccaca tcaaccagat
     3961 cgctcatctg ctgcgtttgc cctaaagcgc gatcgctacc taaggaatcc agcgactcca
     4021 gttcgttcag ctgcgtgcct aattccgcgc gaacggcaag gacattattc agcgagtttt
     4081 tcagtccacg gttggtttta tccaatgccg cagcggcgat ttctttatcc gcttcgctat
     4141 ccgcgaccgg cgttttcagg gctgcgatgg cgctatccag catggcaaaa agattggttt
     4201 cagaagcgct accgtctggc tccgcaactg cgttgctggt aatactgtcg aaaattttgt
     4261 cacccgtgtg ccctatcacc atcgaacgcg aagcatcgac ctgttgctta atactttctg
     4321 ctccaccgac gtatttccct ttctcttcgc taaacggcgc agtctctgtt ttataaccgg
     4381 caaaaatgta gcgcccgtta ccgtcagtgg tgttcgccag attcagcaac tggtcacgaa
     4441 gcccctgaat atccgtagcc agcgaggccc ggtcatcgtc actcaaggtg ccattgctgg
     4501 cttttcctga gcattctgga tagcagtggt gacctggcta agtacactct cttccagtga
     4561 cactttttga atggcgaaag tacgcgccag cgtgtactgg ctgttttgcg cctgtgcctg
     4621 ggagagaact acggcttgtg atgctgcaat gggatcgtca gaagggttaa cgactcgctt
     4681 acctgtcgac atctgctcgc cgtacttcat ccattccgcc tgagaattgg tgatgccacg
     4741 catgttttgc tggtacatca tctgggtact gaaacgcatc ttatctcccc ttagcgaatg
     4801 ttaatcagcg catcaaaaat cgcgtttgcc gtctgcagaa cctgcgcatt cgccaggtaa
     4861 tactgctgaa aacgttgcag atttccgtac tcttcgtcga gattgacgcc agagatagac
     4921 tgctgctgat tggaaagctg cgtcaccaca ttaccttgcg tggcgctact ggttttcaac
     4981 gtcgcggttt tattaccgat atcactcact aacgaggcat aagcgtcgtt aaaggatttc
     5041 gcaccgccca ccgttttact gttgctttgc agatccagca gggcctgacc gttgcggtta
     5101 tcgctatcac ccgcatcttt ttcgctcgcc atcgctattt tcgcttcgtc ggtgattaat
     5161 acatccatgt tgacgatggc gtcacttact ggtttcagcg tgaagctgtc gttaacggca
     5221 ggcgttcctg taaacgtcaa ctccagacca tcaaatgcca ctttaccgtt ggcatccggc
     5281 gtcaccgtaa aagtggtatt gctggcaagg cgggtgacct gccactgatt attatcgaac
     5341 gagattttgt aatctgtcgc cagtaccgcg gaggcatcag ttaccgtggc accgatcgca
     5401 acgtcacctt tgtttttcgt gttttgcaaa accgcgggct taccgatagc aaagaaatct
     5461 tcaccagcat cgccgttggc atcaaatccg gctttgtgtt gggtgttgaa agcctcagca
     5521 aatgccagcg ccagttgtcc aagcgtatta cgcgtctggt ccagatcctg agaacggaat
     5581 gtcagaatgc cgcccagcga cccggtattc agtaatttct ccgggatctc aatattgcct
     5641 gccgtcccat caacgtaagc gacagtcgta cgagaagggt cggcgctgga aggaactgcc
     5701 gccagttgcc gcgccgtact tccctgaacc agtgagtaac cattggccat cgtgatgtta
     5761 taagtgccgc catcctgaac gctgacttct acaccaacaa tctggtttaa ttcgctcacc
     5821 agttgatcgc gttgatccag cagattgtta ggtgacgccc ctgcccccac gcctgtcagg
     5881 cgggagattt ggtcgttcag gctggcgatt tgtttagcgt aattgttgat ctgatcaacg
     5941 ctggcaccta tcgcgatatt gacctgtttg tcctggtcgc gaagatactg atcggtggtt
     6001 ttaaactgat tcaccaaccc ttctgatttc ccaatcagcg cctggcgcgc tgccgggtct
     6061 tccgcgttac tcaccagcgt ttgcaggctg gtgaagaaat cctgcatctg tgttgccagc
     6121 gaagaggtac tggtggagag catattgtcg attttcgaca tctgctcata gcgggcagtc
     6181 aggccgctac tttgcgtctg cgccgcacgt aactggttgg tgataaacgc atcatactca
     6241 cgctgcacac cagaaacgta gacaccattg ccaacccagc cgccagcgcc caacgtgcta
     6301 ttggcctgcg ccataatagt ggtttggcgg gtatatccgg caacgttata gctggagata
     6361 ttattacttg ccgtatttaa cgccgcctgg gccgcgttca gtccgctcat ggcgttatta
     6421 atcaagctgg acatggaggt tccttataag ccttcaatta cagagtatta tcggcagcga
     6481 cccgccggac ttgagttatt caaaacagat tatcaatgtt catactgtag gttttgctca
     6541 ccttgtcgct tatcgatttc atctgctgaa tcatgttggt gagtttgcgg gcatagtgag
     6601 gatcggtggc atagcccgcg tcctgtaggg cctgcgcccc ctgctccgca ctcgtggcgg
     6661 tggtcacggc ggcgtagcgc gggttacgcg ttaacagccc aacgtaatcc gactatgctt
     6721 ccagatacga gctgtagacc cgaaacttcg cttttacttt cttcgcttcg ccgttttcat
     6781 attcagtcgt ggtgatttca gtaactggcc ctttccagtt gccagaggct ttgacaccaa
     6841 acaggttata gctcggctcg ccgttttcgc ggcggatttg ccgttgcccc caaccagatt
     6901 ccagcgccgc ctgagcgagg atcaaatgat gtggcacacc gctttgctgg cttgccagtt
     6961 gggcgggcag cgagagttgc gcgaggaatg ctttactgtc acccggcagc gaatcatcgt
     7021 agttacgtgg cacggccttt tgcaccagct gcgaaagcgc ctgattttga taacgcacca
     7081 cagtttcgag cgggaatttc atcggtgctg ctggcgtgga ctcctctggc aatggttgtt
     7141 ctggcgtcat ctgtttaacc atcatctctg caagccccag acctttgccc gccgtcatct
     7201 gttgggcaat ctgctggtca tacatactgg tatacaggcg agtgtgctcg ctgctgaaca
     7261 ggccatcttt tggtaaagcg tcgcgcatgc ttttcaacat catctgcacg aacatccctt
     7321 ccacctgacg ggccaccgga cggatatttg ccgccggatc ttcgcccgct ttcgccttta
     7381 gttcgttgag tgattgcgca tcccaggccg cacttgccag tagtttgcta tcgctgatca
     7441 tcagatgatt tccagttttg cccgcagaca tcccgcactt tgcattgatt gcagtatgga
     7501 catcagatcc atcggcgtag cgcccagcgc attgagcgcg cgcaccacgt tattgagcct
     7561 ggcgctggaa cgtacgcttt gcagcgaacc gtcgctctgg cgtaaatcga tctgcgtttg
     7621 tggagtaacc acagtctgtc caccaccaaa cggtgtatct ggctggctga cattggcctg
     7681 acgattaact gttactgaga gattcccctg cgctaccgcg cagctgtcga gggtcacttc
     7741 gcgattcatc accaccgaac cggtgcgcga gttaatcact actttagcgt cctgcggggt
     7801 gacattaacc tgcatattct ggatatcggc aaggaagcgg acctgggaac tgttgccact
     7861 cggtacgcgc acctgaatag tccgcgcatc taacgcggtg gcgctgccat atccacgcac
     7921 gcggttgatg gtgtcagcga tttgctgcgc catgctgaaa tcttcgtcgt taagttgcaa
     7981 attaagggta ttcccgacgc caaactggct gggcaattca cgttcaataa ccgcaccatt
     8041 ggtgatccgt ccaccgttca gttggttaac ctgaacactg ctaccgccag cggaggctcc
     8101 tgcgccgcca accagaatat tgccctgcgc cagcgcatac acctgactgt caacgccctt
     8161 aagcggtgtc atcaacaacg tacctccacg caagcttttg gcattaccca tggaagaaac
     8221 caccacatcg atggtttgcc cctgacgtcc aaacggagga agtgacgctg tcaccattac
     8281 cgcagcgacg ttttttagct gcatattggt gcccgtcgga acggtaattc ccagctgtga
     8341 gagcatgtta ttaagcgttt gtgtggtaaa cggcgtctgg gttgtctggt caccggtgcc
     8401 atccagcccc accaccagac catagccaat cagtgagttt tgccttaccc cctgaacact
     8461 ggtgagatcg cgaatacgct cagcctgagc cgccgtcgtg accagtagaa gaattaatgc
     8521 agagagaaat ttaatcacca cagcctcact tacattggcg acaggttaag gaagaaacgc
     8581 tgcaaccagc ccatattttg cgcttcgtta atgtagccat tgcctacgta ttcaatgcgc
     8641 gcatccacca cctgagtaga cggtacggta ttgctgccgc tgatagtgcg tggattaacc
     8701 acgccagaga agcgaataaa ttcggtaccc tgattaatgg caatctgttt ttcacccacc
     8761 acatgcaggt tgccgttgac cagtacctgg tcaaccgtca ccgtcaacgt gccgctaaag
     8821 gtattgctgg cattggcccc gccctttccg ttgaacgtgt taccaccgga ggcttcgaca
     8881 tcggcacgag cgttaccaaa cagcccctgc aaatagcgcg gcacagtatc aaagccaaaa
     8941 ttagttttac cgtcacggct ggcattcgca gaggagcttt tgctggcgct gacgttctcc
     9001 tgcaacacga tggtcagcgt atcgccaata ttgcgtggtc gacgatcttc aaacagcggt
     9061 tgatagccat agttaatcgg ctgagcagac tggaaaatag aaccgttggc gacgggcgtc
     9121 ggaccgggaa ccggttgtgc actggtcgcc ccctgcacca gcggcgtgga gggtatccag
     9181 gcgcagccgg ttagtgaaag caccaacaag ctggaaatgg cataagtatg cgcagcgttt
     9241 ttttgcatgg ctatcatctt caaaaatcag taaaccggtg aacctgccac cggttaagcc
     9301 ttagagttgc gtcagttttt gctgcatctg atcggtggtg gacaccgctt tactgttgat
     9361 ttcgtaagcg cgttgcacct gaatcatatt gaccagttct tccgccacgt tgacgttaga
     9421 cgtttcaaca tacccttgat acagcaatcc cgcgccgttc aggcccggcg tgctttcgtt
     9481 cggtgcacca gaggattgcg tttcggtgta gaggttttcg ccaatgctct ccagcccggt
     9541 gtcattcata aaggtggtga gattgagctg cccaacctga accggagctg cctggccttg
     9601 ttgggttacg ctgaccacgc catcacgacc gatggtgata cttaacgcat tcgccggaat
     9661 ggtgatcgct ggctgcacct gaaaaccacc agccgtcacc agctgcccgt tctgatccac
     9721 ctggaaagag ccgtcacggg tataggctga tgaaccatct ggcaacatca cctggaaaaa
     9781 gccctgccct ttaatcgcga catctttgct gttgttggtc tgcgacagat ttccctggct
     9841 gtgtaagcgt tcagtggcga ccgggcgtac ccccgtgccg atttgtaatc cggagggtaa
     9901 ggtggtttgt tcggaagact gtgcccccgg ctggcgaatg gtttgataaa gcagatcttc
     9961 aaacaccgcg cgctgacgct taaaaccgtt agtactgacg tttgccaggt tgttggcaat
    10021 gacgtccata ttggtttgct gggcgtcaag gcccgttttg gcgatccata atgaactgat
    10081 cataaaatgt cctgtagtgg attagtgaca agcagagcag aagcacgcga cagtgtgaat
    10141 tccccctcac tctaaccctc tccccaaagg ggcgagggaa tagtccgcgc gcttttattg
    10201 caatgtcatc aatcgttcaa ttaactcatc gacagcagtt ggttggcacg gcctgcgtta
    10261 tcatcgacgc tgctgatcac cttcatctgc atttcaaaac gccgcgcgct ggcaatcatg
    10321 tcgctcattg ccgcaacggc attgacgtta ctgccttcca gaacccccga catcacacgc
    10381 aaggttggat ctgcctgcag taccggccct cgcgtggcct gggtttctgc gcttaaacga
    10441 aaaatgccgt cgtcaccgcg ctgcacttcg ctgcccgtgg ctttcaccag tttcagacgc
    10501 cctactggcg caactgtatt tgccggatcg cccggattga gcgccgagat tgtgccatcg
    10561 gcagcaatag tgatttccgc tccttccggc acagcaattg gccccgcctc gcctatcacc
    10621 tgatgcccct gaattgtcag ttgcccggtg ggatcaacct gaatgctgcc attacgcgta
    10681 tacccttcgc tgccgtcagc ggtctgcacg gccagccagc catcctgctg caacgcaacg
    10741 tccagcgggc gcgaggtgta atctatcttg ccgggcgtca tgtctgcacc cggtgttgac
    10801 gccgttacca gcgtgcgcgt gggcagtgaa agcccttcca ccggcactgc gcgcaaggcg
    10861 tttaactgcg cgcgaaaacc gggcgtagag gcattggcca gattactggc ggttaccgcc
    10921 tgttgattca gcgtctggct ggctgctccc atcgcagtat aaattgcgtg atccattgag
    10981 ctatcccgtc agcgattagc gtaagttaac cagcgtgttg aggatctggt cctgggtttt
    11041 gatggtctgg gcgttagact gatagttacg ctgggcaacg atcatattga ccagttcttt
    11101 actgagatcg acgttggacg cttccagcgc accgttggtc agggtgccaa agtttcccgt
    11161 cccggctgtc cccaacagcg ccacgccaga agattgcgtc gcagaccaga cgttgtcgcc
    11221 ttcggatgcc agaccttcgt tgttggcaaa gttcgccagt acaatctgcc ccagcagttg
    11281 agtttgttcg ttggaatagt tgccgacaac cgtaccgtca tcattgattt gataactcac
    11341 cagatcgccc ggtttgtagc cgttctgggg ggttgccaca atattgttag cgccggtatt
    11401 ttgctgcatg gagttgagga agctcagact aaacgtggcg ggttctgcac cgttaattgc
    11461 gccggttgcg atattattcg ccatcgcacc atccactaat gtgccattag cattaaattc
    11521 cagtgttgtc gctgtcttcg caatgctgtt tggatcactg ctatcctggg tgtagacctg
    11581 ccagttatta tccccggtct tcacaaagta gacgctcatg tcatgagcat taccctgact
    11641 gtcgaaaaca gtcaccgaac cttttttgtt atagctatcc gcattgctgg cgctgaatgg
    11701 cgtaacagta ggaagcggat cactggaatt caggttgatc tgcatcgacg ccgtggtggt
    11761 agttttcgct gccatcaggg tattcgggat cgaaatattg gtcggattcg ccccttgctg
    11821 aatagtcggc ggcgtaccgg ttgccgggta acccgtcagc tgtaaacctt gcatattcac
    11881 caggttacgg ttttcatcca gcttaaattg tccgttacgg ctgtagaaca ccgaaccgtt
    11941 gctgtctacc agacggaaaa aaccgttctg gctgatagca acgtccagac ctcgcccggt
    12001 gttggtggtc gtgccatctg taaagtcctg agtgataccg gcaactttta cccccagtcc
    12061 cactttcgaa ccggcaaaca tatcggcaaa agaggccgtg cctgatttaa agccgtaggt
    12121 ggcggagttg gcaatattgt tgccaataac atcgaggtta gtggcggcag cgtttaatcc
    12181 gctaaccgct tgagaaaagg ccatgactga ctcctgaaat gtgaaggctt aaattatctg
    12241 ccgtacttcg tcgagggtgg tggtgccgta agtgccgaga tccagcgtat taccgctgtt
    12301 gccgcggatc acaccctgca ccagagcaaa ctgcagcggc tgggcaacca gttgtgtacc
    12361 accgttactg gcggtaatcg ctacattgta agaaccgttc ggcgcagtgc tgccatcagt
    12421 caacgtaccg tcccaggtga aactgtgaac tccggcggtc agttcaccaa tatcaatggt
    12481 gcgcacaacc gcgccatttt tatcggtgat ggtggccgta actttgtctg ccgcctgttg
    12541 cagctcaaca ccaaacggcg tggtcgtggt cacagcccct tcttcactgc cggttcccgc
    12601 aagaacagtt gtgccgggga tcatcacgcc gtgaccgatc aggttactgg cctgtaacga
    12661 ctggctgtta tcaatctgtc cggaaataga tccgagcatg gtattgagtt tttcaatccc
    12721 gctgaccgtg ctgatttgtg ccaattgcga cgtcagctcg ttgttttcca ttggattggt
    12781 cgggtcctgg tttttcagct gcgccaccag caaagtcaga aaactgcttt gtaaatctgc
    12841 ggcgttgctg cccgtgagcg aactactgct ggtggtactg acgccggtat ttgtcggatc
    12901 ggtggtggtt accgcaatgg acatagcttt ctcctttatt gaccgagcgt aagggttttc
    12961 agcatcatgc ttttcaccgt gttgagcact tcaacattgg cctgatagct gcgtgacgcc
    13021 gacatggtgt taaccatctc tccgacaaca tcaacgttcg gcatttttac gtagcccttt
    13081 gcatccgcca gcggattacc cggttcataa accagtttgt ccggggcctg actttctata
    13141 acatcggcaa cctttacgcc gcctgtcgca gcacctggtg cagcgttaac ctggaatacc
    13201 acctgttttg cccgatatgg ctgtccatcg ggaccggtca cgctatcagc attcgccaga
    13261 ttactggccg ccacgttcag gcgctgggac tgggcagtta acgccgaccc ggcgatatca
    13321 aaaatattca gcagtgccat ccgttaattt ccgctctgta aaacgttcat catgcctttg
    13381 atttgcccgc tcaacgcact aaggctcatc tggtattgca gttctgcggt aggaggcgtc
    13441 agcgcctgcg ccggaatgtg ttgcgttgag gtcatcgtca gagcaaccac actggttgca
    13501 tcccgtccac gttgcatgac ttttttaagt tcactggcaa aatcgatatc gcgcgcctga
    13561 taaccaaggg tatcggcatt ggcgatgttt gctgccagca cttcctgacg ctgggcgcgc
    13621 agattgagcg cctcttgttg aaaacgtaag gcggcgtcga gcttatcgag catatctcct
    13681 ccgcaggtat caaaattctg ccatcagctt aaatgccttt acgcgcgcgt tatcggcgga
    13741 ataaacgcaa aatgggtcgc tatttatgcc gttgatgacc attggggacg ggtacaattc
    13801 acgttgtaga aatagctggg gggcgaaaat gctggcaata aaacgtagcg tggcgatcat
    13861 cgcgatactg ttcagtccgt taagtgcggc gagcaatctc acatcgcaat tgcacacctt
    13921 ttttagcgcc cagctcgtgg gggtaagtga tgaggttcgt gtttctattc gtacagcacc
    13981 caatctacta ccgccgtgcg agcagccatt gctttcgatg agcaataatt cccgcctgtg
    14041 gggcaatgtg aatgtgttgg cacgctgcgg taacgacaaa cgatatttac aggttaatgt
    14101 gcaggccaca ggaaattatg tggttgccgc gatgcccatt gtgcggggag gaaagctgga
    14161 agctggcaat gtcaaactaa aacgcggacg actggatacc ctgccaccgc gtacggtgct
    14221 ggatatcaat caacttgttg atgccattag cctacgcgat ctatcacccg atcaaccaat
    14281 ccaattaacc cattttcgcc aggcatggcg ggtaaaagcg ggacaacgcg tcaacgtgat
    14341 cgccagcggt gacgggttta gcgccaacgc agaaggtcag gcgctgaaca atgcagccgt
    14401 cgcacagaat gcgcggatgc gcatggtatc gggactggtg gtcagcggcg ttgttgatgc
    14461 agatgggaat attcttataa acctgtaagc tgttaaagat tacccgtccc ttgccgataa
    14521 ataagcaaca catgataaaa gcgccctcaa tgaggaataa accatgagta ttgatcgcac
    14581 ttcgcctctg aatcccgtaa gcaccgttca accgcgcgaa accactgacg cgccggtaac
    14641 gaacacccgg gcggcaaaaa caaccgactc caccagcacc agcaccagca ccagcaccag
    14701 tgtgacgtta agcgacgcgc aagcaaaact gatgcaaccc ggcagcagtg atatcaatct
    14761 tgaacgtgtc gaagcgttaa aactggcgat tcgtaacggt gaactaaaaa tggacatcgg
    14821 caaaattgcc gatgcgctga tcaacgaagc tcagcaagac ttgcagagta actgatcgta
    14881 tgacacgtct tgcagagatc cttgaccaga tgtccgctgt gcttaacgat ctcaaaacgg
    14941 taatggatca agagcagcaa catctctcta tggggcagat caacggcagc cagttgcaat
    15001 ggattacaga acaaaaaagc tcactgctgg cgacgctgga ttacctcgaa cagttacgca
    15061 agaaagaacc caatacagca aatagcgttg atattagtca acgctggcag gaaattactg
    15121 tgaaaacgca gcaactacgc caaatgaatc aacataacgg ttggttactg gaaggacaga
    15181 ttgagcgcaa tcaacaggcg ctggaaatgt tgaaaccgca tcaggaaccg acgctatatg
    15241 gggcgaacgg tcagacctca acaacccatc gcggcggtaa aaagatttcg atctgaagat
    15301 tatctccggc ctgcactgca ggccggaatg cattgttaca ccgtccggcg ggcaaattct
    15361 ttaactttga agcccagtac cgccagtgca gcgaagtacg cggcaatccc cgccagcacg
    15421 accgccatta aacgcagtaa gcgccagggc atggtaccca atgaccactc cggcatgata
    15481 tgtaacatac ctaaaagcac gccagacatc accagtaccg ccaccaccag acgcaacaga
    15541 aacgccatcc agccgggttg cggagtaaag attttctgct tacgcaactg ccagtaaagc
    15601 agcgaagcat tcagacacgc cgccagacca atagaaagtg acagcccggc atgtttcaac
    15661 ggaccaataa acgccaggtt catcaattgc gttaaaatca gcgtaacgat ggcaattttc
    15721 actggcgttt taatgtcctg gcgggaataa aagccaggag ccaacacttt cactacaatc
    15781 aggccaatca aacccaccga gtaggcaatt aacgcccgct gggtcatcag cgcatcaaac
    15841 gcggtaaatt taccgtactg gaacagcgaa acggttaacg gacctgaaag aatgcccaac
    15901 gcaaccgcac tcggcagcgc caacaggaaa caaagacgca acccccagtc catcaaacgg
    15961 ttgtattcat catgattgcc actggcaaaa cttttcgaca gcgacggcag caaaatggtg
    16021 ccaagcgcca cgcccagcac accggacgga aactccatta agcggtcggc gtaatacatc
    16081 caagacaccg aaccggaagc aagaaacgag gcaaaaatgg tgttgatgat taaggagatc
    16141 tggctcacag agacgccaag gatcgccggt cccatctgtt tcaccacgcg cattgctccg
    16201 gcatcgtgga agttaatgcg cggcaggacc agcatgccga tcttcttcag gtgcggtagc
    16261 tgatacacca gctgcaggat gccgccgacc gtaacagccc acgccagcgc cagcaccggt
    16321 gggttaaagt acggtgcggc aaacagcgcg aaaccaatca tgctgatgtt aagcagtgtt
    16381 ggcgcaaacg ccggaatcga gaagcggttc cacgtattca gaatcgctcc caccagcgac
    16441 gccagggaga tcagcaagat atagggaaag gtaatcttta gtagctggct ggtcagggca
    16501 aatttgtcag ctgtatcagc gaagcctggc gcggtcacca tgatcaccca cggtgcggcg
    16561 agcatgccag cgaccgtcac aaccgccagc gcaagtgtca gcaggccaga aacataagag
    16621 acaaagaccc gcgtggcgtc ttcaccctgc ttacttttat attccgccag aatcggtaca
    16681 aatgcctggg aaaatgcccc ttcggcaaag atacggcgta acaagttagg aagtttaaaa
    16741 gcgacgaaaa aggcgtcggt tgccatccct gcgccaaaga ttctggcgac aattgcgtct
    16801 cgtgcgaagc caagcacacg cgaaaacatg gtcatcgagc tgacggcggc cagcgatttt
    16861 aataaattca tcggtgttct aatccagacc aacgggcaaa acgcctgcaa gacaggcgct
    16921 gaaagaggcg aatagtctac ctggattatg gtgaattgct accgccagat gttacagggt
    16981 ttattcactc atcgcatcgc gccagatctt gtcaacgata cgttgcgcca gcacggccta
    17041 ttcgccggcg gtttgcggaa ctgtctgatt ttgcacacat tcaatgaagt gccgcgcaca
    17101 gccgacaaat ccacgttgtt caagagtgct ctgccaacca ggaatcggtt tatgcactac
    17161 gccctgcccg cgctcctcac gccattcgcg catatccgta atgtcgatga gcgcaccgtc
    17221 agtcacggcc tgcacggttt cacgctgact tccggcacgg cgatgcatac aggtggtgat
    17281 ctgcaaagga ccggccgaaa aatggtgctc ggcaaacagc atttcgccag cgtcgttagt
    17341 cagtagcgta ccgccatcca gagaggcttt gccgcccgac aaccacagcg cggtatccac
    17401 cacatgcaga taatcatcca gcaacgtgaa ataaagatcg tgtggcccga cactattgct
    17461 acgatgttta tccattctta gcgaagctgc ggtagcgagt tgcgttttta actcaccgta
    17521 gagtggtgcg aaacgacggt taaaaccgac catcaacgtc agttttttcc gcgccgccag
    17581 ttccaccagc cgttcagcat cgcgcagatt ttctgccagc ggtttatcga cacagacatg
    17641 tacacccgca ttgagtaacg tactgaccac gtcaaagtgg ctggcggtgc tggaatgcac
    17701 aaaaaccgca tcgcaactgg cggcaaggct ggataacgaa tcggcataag gaatgcgcca
    17761 gctttcacaa attggcaggg ctttcgcgcg cgtaggcgac caggctcctt gtaacatcca
    17821 gtcagacgct gccgccagca ccggtaacca cgctttttgc gcaatgccgc ctaatcccac
    17881 aacgccgata cgtaattttt tcactttaat ctcccagatg ggccagcaac gaatcaagac
    17941 gctgtttcag ttctgccact tcgatttcca gggcttcgac gcgggcctgt aaatcaccat
    18001 caaccgcgtt cgacatatcc gttaccgccg gctgatcctc tacctcacca ctgaaaagat
    18061 gcatgtagcg gctttcgcgt ttacccggtt cgcgggccag acgcaccaca aaaggaccat
    18121 cttcgcgatt tgccagttgt tccagcgtcg actccaattc cgccatatcg ctgaattcat
    18181 acattcgcgc ggcgcggctg cgcagttcac ccggcgtctg ggcaccacgc aataacaacg
    18241 tggtgattaa cgccacttcc gctgcgctca gtttcagatc gccaaattct gaattacaaa
    18301 aacgttgttc atatttggtg acccgattac caaagccact caccgtgcgc agataatggc
    18361 gtttgaccag attatccagt tgctcctgca cttcagattc actcagattc atgaccggtt
    18421 cacggttcgt tttctgatta caggccgtga ctacaccatt gactgagagc gggtattgct
    18481 ccggcgtggt cacctgcttt tccagcaagc aaccaatcac gcgagcttca agggcagtaa
    18541 gttgatattt catgagattc tccttagcgg ccgggcgtcc agtctggggt agttaatgcc
    18601 gtcagtacgt gatcgcgcca ttgtccatca atcaacagat agtctttcgc atagccttct
    18661 ttttcaaaac ccagtcgcgc cagtaaatca ccgctgcgtt tattgtgcgg catataatta
    18721 gccataatgc gatgaatatg ttgggtgcgc tgcatataac gaatggctgc ggtcagggct
    18781 tcaaacatga gtcctttgcc ctgccatttt tgcccaatcg aataaccgag atagcaggca
    18841 tgaaaagagc cacgaacaac attggaaaaa ttggcaacgc caataatctc tttttcgtcc
    18901 gggtcgaata agccaaagta gaaagctgaa ccttgtttat gaaattcgtt aatcatcccc
    18961 agcctggcct gccagcctga tggataacag tggctttcgt cgcgcactgg ctcccagggc
    19021 ttgaggaaat ggcgattctc tgcgtaataa tccgcaagac gccaggcatc acgatcatgc
    19081 accagacgca cgaccagtcg gtctgtggtt aagcgcactt ttggcacgtt actgcgatag
    19141 ccaaacattc tatacctact ccttcccgta acgtctttaa tacgctgtaa gtattactat
    19201 acctgcgcaa ccagaccctg tgaaaagtcc aaaatgccat tttctgcatt attcacatat
    19261 ttgataaaaa ctctctatca aagcgacgtt ttgataaaaa aatattgtcc ccgaagtgat
    19321 gggaaaagtc ttagcggcaa tgcagaatag aaagtgctca tcttttattt cccgggaggg
    19381 gaaatgtccc gcgtatcgca ggcgaggaac ctgggtaaat atttcctgct catcgataat
    19441 atgctggtcg tgctggggtt ctttgttgtc ttcccgctga tctctatccg cttcgttgat
    19501 caaatgggct gggccgccgt catggtcggt attgctctcg gtctacgcca gtttattcag
    19561 caaggtctgg gtattttcgg cggcgcaatt gccgaccgct ttggtgccaa accgatgatt
    19621 gttaccggta tgctgatgcg cgccgccgga ttcgccacaa tgggtatcgc ccacgaaccg
    19681 tggctattgt ggttttcatg cctgctctcg ggactcggcg gcacgttgtt tgatccgccg
    19741 cgttcggcgc tggtggtgaa actaatccgt ccacaacaac gtggtcgttt tttctcgctg
    19801 ttgatgatgc aggacagtgc cggtgcggtc attggcgcat tgttggggag ctggctgttg
    19861 caatacgact ttcgcctggt ctgcgccaca ggggccgttt tgtttgtgct gtgtgcggcg
    19921 ttcaatgcgt ggttgttacc ggcatggaaa ctctccaccg tacgcacgcc cgttcgcgaa
    19981 ggcatgaccc gcgtgatgcg tgacaagcgt tttgtcacct atgtcctgac gctggcgggt
    20041 tactacatgc tggctgtaca agtgatgctg ccaattatgg tcaacgacgt ggctggcgcg
    20101 ccctctgccg ttaaatggat gtatgccatt gaagcgtgtc tgtcgttaac gttgctctac
    20161 cctatcgccc gctggagtga aaagcatttt cgtctggaac accggttgat ggctgggctg
    20221 ttgataatgt cattaagcat gatgccggtg ggcatggtca gcggcctgca acaacttttc
    20281 accctgattt gtctgtttta tatcgggtcg atcattgccg agcctgcgcg tgaaacctta
    20341 agtgcttcgc tggcggacgc aagagctcgc ggcagctata tggggtttag ccgtctgggt
    20401 ctggcgattg gcggcgctat tggttatatc ggtggtggct ggctgtttga cctgggcaaa
    20461 tcggcgcacc agccagagct tccgtggatg atgctgggaa ttattggcat cttcactttc
    20521 cttgcgctgg gttggcagtt tagtcagaaa cgcgccgcgc gtcgtttgct ggaacgcgac
    20581 gcctgatttg atactccccc ctgactcctc catactgaaa gtgaactgac agccaaatgg
    20641 aggagtcacg tgaagctata catttacgat cactgccctt actgcctcaa agcccgcatg
    20701 attttcggcc tgaaaaatat ccccgtcgaa ttacatgttc tgctcaacga cgacgcagaa
    20761 acacccaccc ggatggtcgg tcaaaaacag gttcccattc tgcaaaaaga tgacagccgc
    20821 tatatgccag aaagcatgga tatcgttcac tatgtcgata aactcgacgg caaaccgtta
    20881 ctgaccggca aacgttcccc tgccattgaa gagtggctgc gcaaggtcaa tggctacgcc
    20941 aacaaactgc tgttgccgcg ttttgccaaa tcggcatttg atgagttttc tactcccgcc
    21001 gcgcgcaaat atttcgtcga caagaaagag gccagcgcgg gtaattttgc cgacctgctg
    21061 gcccactctg acggtctgat taagaatatc agcgatgatt tgcgtgcgct ggacaaactg
    21121 atcgtcaaac cgaacgccgt gaatggagaa ctttcggaag atgatattca gctatttccg
    21181 ctactgcgta atctgacgct ggtagccgga atcaactggc caagccgcgt tgctgattac
    21241 cgcgataata tggcgaaaca gacacaaatc aatttgttat catcaatggc gatttaatcc
    21301 tgacccgccg cggcagcctg tattcgcggg ttgccgccgt tttcagtttt ttctgctggc
    21361 gagtttatcg ctggtgacac atgctgcgtt gtgtcaggat attgacgcag atttcatgtc
    21421 gaggactacc atgaacaagt ttttatttgc cgctgcattg atcgtcagtg gcctgctcgt
    21481 tggctgtaat caactcaccc aatacaccat caccgaacaa gaaattaacc agtcgcttgc
    21541 gaaacataat aatttctcaa aagatatcgg tttacccggc gtggctgacg cccatattgt
    21601 tctgacaaac ttgaccagcc aaattggtcg cgaagagccg aataaggtta ccctaaccgg
    21661 agacgccaat ctggacatga actccctgtt cggtagtcag aaagcgacca tgaaactgaa
    21721 gctgaaagcg ctgcccgtgt ttgataaaga aaaaggtgcg atcttcctga aagagatgga
    21781 agtggtcgat gcgacggtac aaccggaaaa aatgcaaacg gtgatgcaaa cgttgcttcc
    21841 ctatttgaac caggcattac gcaattactt taaccagcaa cctgcttacg tcctgcgcga
    21901 agatggcagt cagggcgaag caatggcgaa aaaactggcg aaaggcattg aagtgaagcc
    21961 aggcgaaatt gtcattccgt ttacggatta atgacgaggg cgcattcgcg ccctttattt
    22021 ttcgtgcaaa ggaaaacgtt tccgcttatc ctttgtgtcc ggcaaaaaca tcccttcagc
    22081 cggagcatag agattaatga ctgcaccatc ccaggtatta aagatccgcc gcccagacga
    22141 ctggcacctt cacctccgcg atggcgacat gttaaaaact gtcatgccgt ataccagcga
    22201 aatttatgga cgggctattg taatgcccaa tctggctccg cccgtgacca ctgttgaggc
    22261 tgccgtggcg tatcgccagc gtattcttga tgccgtacct gccgggcaca atttcacccc
    22321 attgatgacc tgttatttaa cagattcgct ggatcctaat gagctggagc gcggatttaa
    22381 cgaaggcgtg ttcaccgctg caaaacttta tccggcaaac gcaaccacta actccagcca
    22441 cggcgtgacg tcaattgacg caatcatgcc ggtacttgag cgcatggaaa aaatcggtat
    22501 gccgctactg gtgcatggtg aagtgacaca tgcagatatc gacatttttg atcgtgaagc
    22561 gcactttata gaaagcgtga tggaacctct gcgccagcgc ctgactgcgc tgaaagtcgt
    22621 ttttgagcac atcaccacca aagatgctgc cgactatgtc cgtgacggaa atgaacggct
    22681 ggctgccacc atcactccgc agcatctgat gtttaaccgc aaccatatgc tggttggtgg
    22741 cgtgcgtccg cacctgtatt gtttacccat cctcaaacgc aatattcacc aacaggcatt
    22801 gcgtgaactg gtcgccagcg gttttaatcg agtattcctc ggtacggatt ctgcgccaca
    22861 tgcacgtcat cgcaaagaga gcagttgcgg ctgcgcgggc tgcttcaacg ccccaaccgc
    22921 gctgggcagt tacgctaccg tctttgaaga gatgaatgct ctgcagcact ttgaagcatt
    22981 ctgttctgta aacggcccgc agttctatgg cttgccggtc aacgacacat tcatcgaact
    23041 ggtacgtgaa gagcaacagg ttgctgaaag catcgcactg actgatgaca ccctggtgcc
    23101 attcctcgcc ggggaaacgg tacgctggtc cgttaaacaa taaaaatcgt agcttcctgt
    23161 tgtcattagg ttattttacc tgtataaata accagtatat tcaacagggg gctattatgc
    23221 gaattgaagt caccatagcg aaaacttctc cattgccagc tggggctatt gacgccctgg
    23281 ctggcgaact ttcccgccgt attcagtatg cgtttcctga taatgaaggc cacgtatcgg
    23341 tacgttatgc cgcagcgaat aatttatcgg ttattggcgt aacaaaagaa gataaacagc
    23401 gcattagcga aattctccag gaaacgtggg aaagcgccga tgactggttt gtcagcgaat
    23461 aatatgcagt gatttttgtt tttgccgggt cgccccggct tttttatttt acttacaaat
    23521 tttgagtaca aaatttctac acttcctcaa aataattgca caatatgcta aaaaaatatg
    23581 aatgacatgc tgttttattg ttcatataaa tcttaaaagc agaaatttat ctgttccccc
    23641 ctttaaaaat tcggtgaata cccttactta ttggtatact gaagttgctt cagataaaaa
    23701 cgcattgaac ctcgaataac gttgtctagt aacacgaatt agggggccat gatggaaaaa
    23761 aataatgaag tcattcagac tcatccgctc gtagggtggg acatcagcac cgttgatagc
    23821 tatgatgcgc tgatgttgcg tttgcactac cagaccccaa ataagtccga gcaggaaggg
    23881 actgaagttg gtcagacgct ctggttaacc actgatgttg ccagacaatt tatttcgata
    23941 ttagaagcag gaatcgccaa aattgaatcc ggtgatttcc cggtaaacga gtatcggcgt
    24001 cattaacatg acatgaccat ccacccaaaa ggcacctcac cggtgccttt accatttctg
    24061 ggttgtatat ctgcattcag ttaattactc tgcttaaacc atgagagcag agagaaaaaa
    24121 atgaaatacg atctcatcat tattggcagc ggttccgtag gcgctgccgc cgggtattat
    24181 gcaacccgcg ccggtttaaa cgtgctaatg accgacgccc atatgccacc gcatcaacac
    24241 ggcagccacc acggcgatac gcgattaatt cgccatgctt atggtgaagg cgaaaagtat
    24301 gtcccgttgg tcctccgcgc gcaaacgctg tgggatgaac tttcccgcca caacgaagat
    24361 gatcccattt ttgtacgctc tggtgtcatt aacctcggcc cggctgactc cgcatttctc
    24421 gccaacgtcg cccacagcgc cgaacagtgg caactcaacg ttgaaaagct cgatgcgcaa
    24481 gggattatgg cccgctggcc agaaatacgc gtcccggaca actacatcgg cttatttgag
    24541 actgattccg gttttttgcg cagcgaactg gcgattaaaa cctggatcca actggcgaag
    24601 gaagcgggct gtgcgcaact gttcaactgc ccggtcaccg caattcgtca tgacgatgat
    24661 ggcgtaacta ttgaaaccgt tgacggtgag tatcaggcga aaaaagcgat tgtctgcgcg
    24721 ggaacatggg taaaagacct gctcccggag ctgcctgtcc agcctgtacg taaagtattt
    24781 gcctggtatc aggccgatgg ccgctatagc gtgaagaata aattcccggc gtttaccggt
    24841 gaactgccca atggcgatca atattatggt tttccggcag aaaacgacgc gttgaagatt
    24901 ggcaaacata acggaggcca ggttatccat tcagcggatg aacgtgttcc gtttgcggaa
    24961 gtggtcagcg atggttcgga agccttcccg ttcttgcgca atgtattgcc gggtatcggt
    25021 tgctgcctgt acggcgctgc ctgcacctat gataattcgc ctgacgaaga ttttattatc
    25081 gataccctac ccggccacga taatacactg ctcattaccg gcctgagtgg gcacggtttt
    25141 aaatttgcgt cagttttagg ggaaatagct gccgattttg cgcaagacaa aaaaagcgat
    25201 tttgatttga cgccattcag gctttcccgc ttccaataat catcattacg gcctccagaa
    25261 tgaggccgtt tttacaggag tatttaatgc gtcggctgtt gcactatctc atcaataata
    25321 ttcgcgagca tctgatgctt tatctttttc tctggggatt gttggcaatt atggatctga
    25381 tctatgtgtt ttatttttag ttttccgcac tgacataaac tgacaaatat ttaatctccc
    25441 tttcatttag cgtgaaaaat tgaatgagat tattcctgct tctacagcac tcaccagaca
    25501 tctctgatgc tgaacccact aaacttatga agattctttc attattaatt caaaattatt
    25561 gaaataaaaa taaatatcat tcaatttgtt atttttgtgt tgcaattcat tctccgacac
    25621 gccattttct ttctctcgca aattcgtatt ggatgcactt atgtcattca caaatacccc
    25681 tgaacgctat ggtgttatat ctgcggcctt ccattggcta agcgcaatta ttgtctacgg
    25741 catgtttact ttaggtctgt ggatggtcac gctcagttat tacgatggct ggtatcacaa
    25801 agcacccgaa ctgcataaaa gcatcggtat tttgttaatg atggggctgg ttattcgcgt
    25861 tctgtggcgt gtcatatctc caccgccggg tccgctgccg agttattcgc ccatgactcg
    25921 tcttgccgca aaggctggac atctcgcatt gtacctattg ctgttcgcca tcggcatcag
    25981 cggctatctg atctcaactg ccgatggtaa accgatcagc gtttttggct ggtttgacgt
    26041 ccccgcgacc cttgccgacg ctggcgcaca ggcagacttt gccggtgccc tgcatttttg
    26101 gctcgcgtgg agcgtcgtag tactgtccgt tatgcacgga tttatggccc tgaagcatca
    26161 tttcatcgat aaagacgaca ctctgaagcg catgctggga aagtcgtcat ctgactatgg
    26221 agtataaaaa atgaaaaaaa gcctgcttgg tttaaccttc gcgtccctga tgttctctgc
    26281 cggttcagcg gttgccgccg attacaaaat tgacaaagaa ggtcagcacg cctttgttaa
    26341 tttccgcatc cagcaccttg gctatagctg gttatacggc acctttaaag atttcgacgg
    26401 tacttttacc tttgacgaaa aaaatccggc tgccgataaa gtgaatgtga caattaacac
    26461 caccagcgtc gatactaatc acgccgaacg cgataaacat cttcgcagtg cagatttcct
    26521 caataccgca aaatatccac aggcaacatt cacctccacc agcgtgaaga aagacggtga
    26581 cgaactggat attaccggcg atctgacgct gaatggcgta accaaacccg tcacgctgga
    26641 agcgaaatta attggtcagg gcgacgaccc atggggtggt aaacgtgcag gcttcgaggc
    26701 cgaaggcaaa attaagctca aggacttcaa tatcaagaca gatttaggtc cagcttctca
    26761 ggaagtggat ctgattattt cagtggaagg cgtacagcag aagtaagcgt gaaatgttgc
    26821 cggatggcac gcatccggca atgatattta ttctgttgga tcaggaatgc ccagtgttgt
    26881 attcagacgt ccacgagact tattaaagat cttattgcca ttttcacgtc ctgcccgacg
    26941 gcgtcgctgt tcttctggcg gtaacgcgct ttcttcgcag caaatctcac tacaacaacc
    27001 tttgtatttt tccgcgcata ctggacactg aataaacagc aggtggcagc catcattttt
    27061 acagttggta tggctgtcgc acggcgcgcc gcactggtgg caatgcgcga taatctcatc
    27121 cgatatacgt tcgcccatcc gctcgtcaaa aacaaaattt ttgccaataa aacgcaccgg
    27181 caagccctgc tcgcgcgcct tacgggcgta ttcaataatt ccgccctcga tatgccagac
    27241 tttgttgaat ccgttatgtt tcatccaagc actggccttt tcacaacgaa tgccgccggt
    27301 gcagtacatg acgatttttt tatctttatg tgcctgcatc atctcgactg cttttggcag
    27361 ctgctcacgg aaggtatctg ccggaatttc gaacgcgttt tcaaagtgcc ccacttcata
    27421 ctcatagtgg ttac
//
LOCUS       DADLFA010000037        27167 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000037 DADLFA010000000
VERSION     DADLFA010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27167
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.037"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(37..1335)
                     /gene="eno"
                     /locus_tag="JQH65_001340"
     CDS             complement(37..1335)
                     /gene="eno"
                     /locus_tag="JQH65_001340"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAY6138810.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(1423..3060)
                     /gene="pyrG"
                     /locus_tag="JQH65_001341"
     CDS             complement(1423..3060)
                     /gene="pyrG"
                     /locus_tag="JQH65_001341"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HAY6138811.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(3288..4079)
                     /gene="mazG"
                     /locus_tag="JQH65_001342"
     CDS             complement(3288..4079)
                     /gene="mazG"
                     /locus_tag="JQH65_001342"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HAY6138812.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4150..4485)
                     /gene="mazF"
                     /locus_tag="JQH65_001343"
     CDS             complement(4150..4485)
                     /gene="mazF"
                     /locus_tag="JQH65_001343"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HAY6138813.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKRIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(4485..4733)
                     /gene="mazE"
                     /locus_tag="JQH65_001344"
     CDS             complement(4485..4733)
                     /gene="mazE"
                     /locus_tag="JQH65_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HAY6138814.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(4811..7045)
                     /locus_tag="JQH65_001345"
     CDS             complement(4811..7045)
                     /locus_tag="JQH65_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HAY6138815.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7093..8394)
                     /gene="rlmD"
                     /locus_tag="JQH65_001346"
     CDS             complement(7093..8394)
                     /gene="rlmD"
                     /locus_tag="JQH65_001346"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAY6138816.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     SALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8451..11207
                     /gene="barA"
                     /locus_tag="JQH65_001347"
     CDS             8451..11207
                     /gene="barA"
                     /locus_tag="JQH65_001347"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HAY6138817.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11439..12743)
                     /gene="gudD"
                     /locus_tag="JQH65_001348"
     CDS             complement(11439..12743)
                     /gene="gudD"
                     /locus_tag="JQH65_001348"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HAY6138818.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIMIIK
                     DNSGHTGVGEIPGGEKIRKTLGEYKNVLTLVRNTFADRDAGGRGLQTFDLRTTIHVVT
                     GIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGDRKATPLPYQSQPDDSC
                     DWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAESIVALAQRFPQA
                     RITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMAEFRRATGLPTAT
                     NMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGLTWGSHSNNHFDI
                     SLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQVPEKPGLGVEIDM
                     DQVMKAHELYQKHGLCARDNAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12764..14104)
                     /locus_tag="JQH65_001349"
     CDS             complement(12764..14104)
                     /locus_tag="JQH65_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HAY6138819.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELHVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14106..15476)
                     /locus_tag="JQH65_001350"
     CDS             complement(14106..15476)
                     /locus_tag="JQH65_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY6138820.1"
                     /translation="MFRSATTSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRAT
                     LSIAGSEMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWS
                     MFTLLQGFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSI
                     FNSAQYFATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQ
                     KELEYIAAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYF
                     FITWFPVYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKT
                     PIVMGMLLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSG
                     GLFNMFGNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIEL
                     KPVAGQ"
     gene            complement(15893..16342)
                     /locus_tag="JQH65_001351"
     CDS             complement(15893..16342)
                     /locus_tag="JQH65_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAY6138821.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLCYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENLEPETESNPWVEQWGTLLS"
     gene            complement(16360..17142)
                     /gene="truC"
                     /locus_tag="JQH65_001352"
     CDS             complement(16360..17142)
                     /gene="truC"
                     /locus_tag="JQH65_001352"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HAY6138822.1"
                     /translation="MLEILYQDEWLVSVNKPSGWLVHRSWLDRDEKVVVMQTVRNQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17142..17471)
                     /locus_tag="JQH65_001353"
     CDS             complement(17142..17471)
                     /locus_tag="JQH65_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HAY6138823.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQHALILAELEKLDA
                     LFADDAS"
     gene            complement(18093..18638)
                     /gene="syd"
                     /locus_tag="JQH65_001354"
     CDS             complement(18093..18638)
                     /gene="syd"
                     /locus_tag="JQH65_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HAY6138824.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18706..19554
                     /gene="queF"
                     /locus_tag="JQH65_001355"
     CDS             18706..19554
                     /gene="queF"
                     /locus_tag="JQH65_001355"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HAY6138825.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19666..21030
                     /gene="ppnN"
                     /locus_tag="JQH65_001356"
     CDS             19666..21030
                     /gene="ppnN"
                     /locus_tag="JQH65_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HAY6138826.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            21587..22876
                     /locus_tag="JQH65_001357"
     CDS             21587..22876
                     /locus_tag="JQH65_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HAY6138827.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            22934..24301
                     /gene="sdaB"
                     /locus_tag="JQH65_001358"
     CDS             22934..24301
                     /gene="sdaB"
                     /locus_tag="JQH65_001358"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HAY6138828.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            24323..25168
                     /gene="xni"
                     /locus_tag="JQH65_001359"
     CDS             24323..25168
                     /gene="xni"
                     /locus_tag="JQH65_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311685.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HAY6138829.1"
                     /translation="MRGLFPISHPAVACSGIECYPYRLIFKGVIVAVHLLIVDALNLI
                     RRIHAVQGSPCVETCQHALDQLIMHSQPTHAVAVFDDENRSSGWRHQRLPDYKAGRPP
                     MPEELHDEMPALRAAFEQRGVPCWSTSGNEADDLAATLAVKVTQAGHQATIVSTDKGY
                     CQLLSPTLRIRDYFQKRWLDAPFIDKEFGVQPQQLPDYWGLAGISSSKVPGVAGIGPK
                     SATQLLVEFQSLEGIYENLDAVAEKWRKKLETHKEMAFLCRDIARLQTDLHIDGNLQQ
                     LRLVR"
     gene            complement(25273..26410)
                     /gene="fucO"
                     /locus_tag="JQH65_001360"
                     /pseudo
     CDS             complement(25273..26410)
                     /gene="fucO"
                     /locus_tag="JQH65_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
     gene            complement(26438..27085)
                     /gene="fucA"
                     /locus_tag="JQH65_001361"
     CDS             complement(26438..27085)
                     /gene="fucA"
                     /locus_tag="JQH65_001361"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HAY6138830.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIAFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
BASE COUNT         6573 a         7282 c         6547 g         6765 t
ORIGIN      
        1 cggaggctgg catttttaaa tcagataaag tcagtcttat gcctggcctt tgatctcttt
       61 acgaccgttg tacggtgctt tttcgcccag agcttcttcg atacgaatca gctggttgta
      121 tttagcaaca cggtcagaac ggctcataga accagttttg atctggcctg cagcagtacc
      181 aacagccagg tcagcgatgg tagcgtcttc agtttcgcca gaacggtgag agataactgc
      241 agtgtagcca gcatctttcg ccatcttgat tgcagccaga gtttcggtca gagaaccgat
      301 ctggttgaat ttgatcagga tggagttagc gatacctttt tcgatacctt ctttcaggat
      361 cttggtgttg gttacgaaca ggtcgtcacc aaccagctgg attttgtcgc ccagaacttt
      421 ggtctggtat gcgaaaccgt cccagtcaga ttcgtccaga ccgtcttcga tagaaacgat
      481 cgggtactgt ttggtcagtt cttccaggaa gtgagtgaat tcttcagagg tgaacgcttt
      541 gttgccttcg ccagccagaa cgtatttacc atctttgtag aattcagaag ctgcgcagtc
      601 catcgccaga gtgatgtctt tgcccagttc ataaccagca gctttaacag cttcagcgat
      661 aacagccaga gcttcagcgt tggaacccag gttcggcgca tagccacctt cgtcaccaac
      721 agcagtgttc atgcctttcg ctttcagaac ttttgccagg tgatggaaaa cttcagaacc
      781 catgcggatg gcttctttca cagttttcgc gccaaccggc tgaatcatga attcctggat
      841 atcaacgttg ttgtcagcgt gctcaccacc gttgatgatg ttcatcatcg gaaccggcat
      901 agagtatttg cccggagtac cgttcagttc agcgatgtgc tcgtacagcg gcataccttt
      961 agcagctgca gcagctttgg cgttagccag agatacagcc aggattgcgt tcgcgccgaa
     1021 tttggatttg ttttcggtgc cgtccaggtc gatcatgatc ttgtcaatgc cagcctgatc
     1081 tttagcatct ttgccaatca gcgcctgagc gatcgggccg tttaccgcag caacagcttt
     1141 ggttacgcct ttacccagga aacgggattt gtcgccatcg cgcagttcca gagcttcacg
     1201 ggaaccagta gaagcacctg acggagcagc tgccataccg acgaaaccac cctccagatg
     1261 tacttcggct tcaacagtcg ggttaccacg ggagtcgatg atttcacgac cgatgatttt
     1321 tacgattttg gacattaggt tttcctcaag tcactagtta aactgaaact ccagacaaac
     1381 aacacgtacc cagggtacgc gttgccgttc taactttttt acttacttcg cctgacgttt
     1441 ctggaactcg ctggcggctt tcacaaagcc tgcaaacagc gggtgaccat cacgtggagt
     1501 agaagtaaac tccggatgga actggcaagc cacgaaccac gggtgattcg gaacttcgat
     1561 gatctcgacc aactgatcat ccccggaacg gcccgcaacg cgcagacctg catcttcaat
     1621 ctgtttcaac agcatgttgt tgacttcgta acggtgacga tgacgctcaa caattgtcgg
     1681 cgcattgtac agctggcgaa ccaggctatc gtcaaccaac tggcactgct gtgcgccgag
     1741 acgcatggta ccgccgagat cgctcttctc gctacgaact tcaacgttgc cgttttcatc
     1801 gcgccactcg gtaatcagcg ccacaaccgg gtacttacag tctggcacaa attccgtaga
     1861 gttggcgttc tccatgttgg caacatggcg agcgtaatca attaacgcca cctgcatacc
     1921 caggcaaatg cccagataag gaatattgtt ctcacgcgca aaacgcgcgg tcgtaatcat
     1981 gccttctacg ccacgatagc cgaaaccgcc aggtacgagg attgcgtcca gacctttaag
     2041 gatttcaacg ccgcgcgttt caacatcttg tgaatcgatc agtttgatgt tgacgctgac
     2101 acgattcttc agcccaccgt gtttcagtgc ttcgatcact gatttataag catccggcag
     2161 ttcaatgtac ttgccgacca taccgatggt gacttcactt accgggttcg cttcttcgaa
     2221 gataacctgt tcccattcgg acagattcgc ttccgggcag tttaagctga atcgtttaca
     2281 aatataatcg tccagcccct gagatttcaa caggcccgga attttataga tggaatcgac
     2341 gtctttcaga gaaataaccg ctttttccgg aacattacag aacaatgcaa tcttcgcacg
     2401 ttcgttcgcc ggaacagcgc gatctgaacg acaaatcagg atgtcaggct ggataccgat
     2461 ggagagcagc tcttttacag agtgctgagt cggtttggtt ttgacttcac cagacgctgc
     2521 catgtacggc accagcgtca ggtgcataaa cagagtgtgc tcacggccaa tttcaacagc
     2581 catctggcga atcgcttcga ggaacggcaa ggattcgata tcacctactg taccgccgat
     2641 ttctaccagt actacgtcat gaccttcgcc accttccagc acgcgctctt tgattgcgtt
     2701 agtgatgtgc ggaataacct gcacggttgc gccgaggtag tcaccgcggc gttctttacg
     2761 cagaacgtca gagtagatac gacccgtggt gaagttgttg cggcggctca ttttggtacg
     2821 aatgaaacgc tcgtagtgcc ccaggtccag gtcggtttca gcgccgtctt cagtaacgaa
     2881 cacttccccg tgttggattg ggctcatagt acctggatcg acgttgatgt acggatccag
     2941 tttcatgatg gtcacattga ggccacgggc ttcaagaatg gctgcgaggg aggctgcggc
     3001 aatgccttta cccagagagg atacgacccc gccggtcaca aaaatatagt tcgttgtcat
     3061 gctgaacctg agaagttagg ttgaaaagac gatggaataa ccaggacggg aaagcagtat
     3121 acccgaacat gacctgtgcc acaaactttc attatccctc ctcttcgcca gcgcactatt
     3181 gaaatcagga gtgagaaaat agcccctttg gggtaaatgt ttttgacgca aatcaagcgc
     3241 ttgtcattta aaaaatgaca caaatggcac ttgaccgcgt aattccctta gagatcaatt
     3301 tcctgccgtt ttacctgttg ccagacttct tccattgttt cgaggtcaac acctgtcatt
     3361 tccagtccac gcgcggcaac aatacgctcc acttcgcgaa aacgacgctc gaatttttcg
     3421 ttcgcttttt gcaatgcgat ttctgctttc gtccctaaat ggcgagccag attaaccgtg
     3481 gcaaacagca ggtcccccat ttcctcctcc agtttagcct ggtcgacaac agcctgccgc
     3541 gcttcgtaca tcacctcgtc gatctcttcg tagactttat caaccaccgg gccaagcgtc
     3601 gtccagtcga agccaacgtt ggcgcaacgt ttctggattt tttgcgcccg cattaaagcc
     3661 ggtaaactac gaggaatatc gtccagcgcc gaatgctgcg ctttctgcgc gcgctcttcg
     3721 gttttgattt gctcccaacg ggcaagcact tcactactgt tttcggcaga actatcagca
     3781 aaaacatgcg gatggcgacg ctctaattta tcgctaatag cagcgcaaat atcattaaag
     3841 tcaaagcgcc cttcttcctg agccatttgc gcgtaaaaca ccacctggaa tagcagatcg
     3901 cccagttcac cgcgaagatc gtcaaaatct tcacgggcga tggcgtccag cacttcgtag
     3961 gtttcttcaa gggtgtaagg cgcaatggtg gcaaatgtct gctctttatc ccacgggcag
     4021 ccgttttccg gatcgcgcag gcgctgcata atagtgagca aacgatcgat ttgattcatt
     4081 gaattgtcct gaaaattgcg ggtctgtcag gtggaaacct gtgaccagaa tagaagtgag
     4141 ttagtaacac tacccaatca gtacgttaat tttggcttta atgagttgta attcctctgg
     4201 ggcaactgtt cctttcttcg ttgctcctct tgcccgccag gcgattcttt ttacctgatc
     4261 agctaacgct acgccatcac gttcctgacc ggataaaaca acttcgaacg gatatccttt
     4321 tgattgcgtt gtacaaggaa cacacagaca catacctgtt ttgttgttgt acatgaaagg
     4381 actcaggaca acagctggac gatgtccagc ttgctcgcta ccttttgtcg ggtcaaaatc
     4441 aacccaaatc agatcgccca tatcgggtac gtatcggctt accattacca gacttcctta
     4501 tctttcggct ctccccagtc gatattctcg tggaggtttt ccggcgtgat gtcgttgacc
     4561 agttcagcaa gcgtaaatac gggctcttta cgcactggct caataattaa tttgccatcc
     4621 accaggtcaa tcttcacttc atcatcaata ttcagattga gcgcctgcat taacgtagcc
     4681 gggatccgca ccgccggtga atttccccaa cgctttacgc tactgtggat catcaccctt
     4741 tcctcaaacc gctatcatat gtagatacag tatatatcaa tctacattgt agatacgagc
     4801 aaatttctgc ctaactcccg tgcaaccgac gcgcgtcgat aacatccggc acctggttga
     4861 gtttacccag cacgcggccc agcacctgca ggttgtaaat ctcaatggtc atgtcgatgg
     4921 tcgccagttg ctgtttggtg tcgctacggc tggcaacgcc aagtacgttc accttctcgt
     4981 tggcgagaat ggtcgtgata tcacgtaaca acccactacg atcattagcc accacgcgga
     5041 ccaccagcga atatccggcg gagtagctct caccccatac cgcgtcaaca atgcgttctg
     5101 gcgcatgaga gcgcagttcc gccagttgtt cgcaatcggc gcggtgtact gaaataccgc
     5161 gcccctgggt aatgaagccg acaatctcat ctccaggaat cggctggcag cagcgcgcga
     5221 tgtggtgcat caggttgcca acaccttcga ctaccacgcg accgttatct ttactgcggt
     5281 tttgcggcgt gtagcttttt tgctgaagtt gcttcagcgc ggcggcgtcc tgctcttcgg
     5341 cactcggctt attaaattgc gattgcagga agttcaccat ctgattgaga cggatatccc
     5401 cgccaccaat cgccgccagc aactcgtcga catcattaaa gttgtaacgc ggcagcagat
     5461 gtttttctgc ttctttcagg ctgatcccca gatgttccag ctcgtcgtca aggatttgcc
     5521 gcccagccag aatgtttttg tcacggtcct gtttacggaa ccaggcgtga attttcgaac
     5581 gcccacggct ggttgtgacg taaccgaggt ttgggtttaa ccagtcacgg ctggggttcg
     5641 gctgtttctg ggtgataatt tcaatctggt cgcccatctg cagctggtag gtgaacggca
     5701 caatgcgccc gccaattttt gccccgatgc agcggtgtcc gacatcactg tggatgtggt
     5761 aagcgaagtc cagcggcgtt gatcccgcag gcaaatcaac gacatcacct ttcggcgtaa
     5821 agacatacac ccggtcgtca aagacctggc tgcgtacttc gtcgagcatt tcgccggaat
     5881 cagccatctc ttcctgccac gcaatcagtt tacgcagcca ggcaatccgg tcttcatgtc
     5941 ccgaacgtgc gccgccagct gccgcgccct ctttatattt ccagtgcgca gcaacaccca
     6001 actctgcatc ttcatgcatc tgtttggtgc ggatttggat ctcaacggtt ttgccacctg
     6061 gccccagaac cacggtatga atagactgat aaccgtttgg tttcgggtta gcgacataat
     6121 cgtcaaactc atccggcagg tggcgatagt gagtgtgcac tatccccagt gcggcataac
     6181 aatcctgtaa acgctcggcg acaatacgta ccgcacgcac atcaaacagc tcatcgaagg
     6241 cgaggttctt tttctgcatt ttgcgccaga tgctgtagat gtgtttcgga cggccataca
     6301 cttcagcttt aacgccttca gctttcatct cagcgcgcag atgaccgaca aactcttcga
     6361 tatagtgttc gcggtcgaga cggcgttcat gcagcagttt tgcgatgcgt ttgtattcgg
     6421 tcgggtgcag ataacggaag cagtaatctt ccagctccca tttcagttgc ccaatcccaa
     6481 gacggttcgc caacggcgcg tagatattgg tgcactcttt tgccgccagt acgcgttcat
     6541 cttccggcgc atcttttact tcgcgcagat gagcaatacg ctccgccagt ttgatgacca
     6601 cgcagcgaaa atcatcgacc atcgccaata acatccggcg aacgttatcg acctgttcgg
     6661 aggaaacaga atcagtgtgc gtcgctttca gctggcggat cgccgccata tcacgcacgc
     6721 cgtgaataag gttaacgaca gacttaccga cgctctcacg cagcacatct tcgctgacta
     6781 cgttggcatc ggccagaggg aacagcagcg ccgcccgcag cgtgtcaatg tccatactta
     6841 atgtcgagag gatctccacc atctcaacac cacgccacaa taacagactg gcatccggat
     6901 gcccctgcgt ctgctgcaga caatacgccc aggtttcggc taagcactca cacgacttct
     6961 ggctggtaat acccagactt gcgatccatt tttccggatc aaattcacca gccttattga
     7021 tatgtgcact tcttaccgca accattgtcc tctcctttag ggaccagacc tgccgaaatc
     7081 ggcaaatcgt aactatttaa cgcgcgagaa aagtaccatc gattccagat gtcccgtgtg
     7141 tgggaacata tccagcatcg ccagtcgcgc aatggtatat cctgctttta ataacgcttc
     7201 gctatcccga gccagcgttg cagggttaca ggatacataa actatacgaa taggttccag
     7261 ttttataatt tgctgcataa cacctgcggc acctgctcgc gccgggtcca gcaacacttt
     7321 atcgaagccg tttttcgccc acggctgctt tgtgacatct tcttcaagat tttcgtgata
     7381 aaacgtcaca ttctgtaagc cattaagacg cgcattctgc tggccttttt ccaccagcgc
     7441 cggaacacct tctacaccga ccacactggc agcttgtgtc gccaatggca gtgtaaagtt
     7501 gcccataccg cagaacagat ccagtacgcg atcttcaggt tgcacatcca gccattccaa
     7561 cgcactcgct accatttttt ggttcacacc cgcattgacc tgaataaaat cgcgcgggct
     7621 aaaagttaag cgcaacccgt tagagtcata ccagggcatc tcaccagaga cggtttcgag
     7681 tatctcacta tcgggggcga gatacagatc caggccttca gaatgcgaaa agcgttccag
     7741 tttttcgcga tctgccgaac ttagcggtgc ggtatggcgc aaaatcatca gcgtgccgct
     7801 ggttgcctgt accagttcaa catgaccaag atggcgcatt gcttgtaagc tgcccaggca
     7861 tgccctgact ttgggcagca atgcttcaag ttggggcact aaaatgggac attgtttaac
     7921 gtcgacaatg tcactggagc ccgctttgcg aaaccccatc tgaagttgct gtgttttcgg
     7981 taagtagttc agacttaaac gcgcgcgacg gcgatagccc cagggaacat cggcgatcac
     8041 ttcagagact tcgtgtttca ttaatcgggc gagtgccgca cttttgcttc gctgctgtaa
     8101 atccacgctg gcgtgttgtt gctgacagcc gccgcatacg ccaaaatgag gacagcgtgg
     8161 cgtttcgcgt tccgggctat cgcttaaccg gcgtacgact ttagcgcggg catactgttt
     8221 tttatcttca gtaacagtaa cttccgcgtt ttcctgcggc aataatccgg ggataaatag
     8281 cgttttgccg ttatgtcgcg ccacgccctg accaaaagag tcgaggtcgt tgactgaaac
     8341 ggttatgatc tgacgcgtcg tcgtgcgtcg ttttgcagag tagaattgcg ccattggcga
     8401 gactttctca atttaacagt gtgaccttaa ttgtcacata acggaactcc atgaccaact
     8461 acagcctgcg cgcacgcatg atgattctga tcctggcacc gaccgtcctt attggtttat
     8521 tgctgagtat atttttcgtc gtgcatcgct ataacgactt gcagcgtcaa ctggaagatg
     8581 caggtgccag cattattgag ccgcttgcag tttctactga atatggcatg agcctgcaaa
     8641 atcgcgaatc tatcggtcag ttaataagcg tactgcatcg tcgccattcc gatattgttc
     8701 gcgcgatttc ggtttatgat gaaaataacc gactctttgt cacctccaat tttcatcttg
     8761 atccctcatc aatgcagctc ggcagcaacg tgccgtttcc tcgccagctc actgtcactc
     8821 gtgacggcga tattatgatc ctccgcacgc cgattatttc tgagagttac tcccccgacg
     8881 aatcgcccag tagcgatgcc aaaaatagtc aaaatatgtt gggatatatt gcgcttgagc
     8941 tggatcttaa atcggttcgc ttgcagcaat ataaagagat ctttatttcc agcgtgatga
     9001 tgctgttttg tatcggtatt gcgcttattt ttggctggcg cttaatgcgc gatgtaaccg
     9061 gtccgattcg caacatggtg aataccgtcg accgcatccg tcgcgggcaa ctcgacagcc
     9121 gggtggaagg atttatgctc ggcgagctgg atatgctgaa aaacggtatc aactcgatgg
     9181 caatgtcgct ggctgcttat cacgaagaga tgcagcacaa tatcgaccag gcgacgtccg
     9241 atctgcgtga aacgctggag cagatggaaa ttcagaacgt tgagttggat ctggcgaaaa
     9301 agcgcgccca ggaagcggcg cgtattaaat ccgagtttct ggcaaatatg tcacacgagc
     9361 tgcgtacacc actgaatggt gttattggct ttacccgcct gacgctgaaa acagaattaa
     9421 caccaacgca gcgcgatcac ctgaatacga ttgaacgttc ggcaaataat ttgctggcaa
     9481 ttattaatga tgttctcgac ttctcgaaac tggaagcagg taagctgatt ctggaaagta
     9541 ttccattccc actacgcagc acgctggatg aagtcgttac tctgctggca cattcttctc
     9601 acgataaagg gttggaactg acgctcaata ttaaaagcga cgtgcctgat aacgtgatcg
     9661 gcgacccact gcgattacag caaatcatca ctaacctggt ggggaatgca attaaattca
     9721 ccgagaatgg caacattgat attctggtag aaaaacgtgc gctgagtaat accaaagtgc
     9781 agattgaagt gcagattcgg gataccggca ttggtattcc tgaacgcgat caatcgcgct
     9841 tattccaggc cttccgacag gctgatgcca gtatttcccg ccgtcatggt ggcaccgggc
     9901 tggggctggt gattacacaa aaactggtta atgaaatggg cggcgatatt tcgttccata
     9961 gccagccgaa tcgcggttca actttctggt tccacattaa tctcgatctg aacccgaaca
    10021 ttattatcga agggccatcc acccagtgcc tcgcaggtaa gcgcctggcc tatgtcgaac
    10081 caaactccgc agcagcgcaa tgcacgctgg atattttaag tgaaacgccg ctggaagtgg
    10141 tttatagccc aacgttctcc gcgctgcctc ccgcgcatta cgacatgatg ttgttaggca
    10201 tcgcggtgac cttccgcgag ccgctaacaa tgcaacatga gcgattagcg aaagcggtat
    10261 cgatgaccga tttcctgatg ctggcacttc cttgccatgc acaagtcaat gctgaaaaac
    10321 tcaagcaaga tggtatcggc gcgtgtctgc tgaaaccatt aacacctaca cgcctgttgc
    10381 ctgccctgac ggaattttgt catcacaaac aaaacacgct tttgcctgta accgatgaaa
    10441 gtaagctggc aatgacagtc atggcggttg atgacaaccc cgctaacctg aaacttatcg
    10501 gcgcattgct ggaagatatg gtgcaacatg tggaactttg cgatagcggg catcaggcgg
    10561 ttgaacgggc gaaacagatg ccgttcgatt tgatcttaat ggatattcaa atgccggaca
    10621 tggatggcat tcgggcctgc gaactcatcc accagctccc ccatcagcaa caaacgccgg
    10681 ttatcgcggt aacggcgcat gcaatggccg ggcaaaaaga gaagctgctt ggcgcaggga
    10741 tgagcgatta tctggcgaaa ccgattgaag aagagcgatt gcataatttg ttgttgcgct
    10801 acaagcctgg cagcggtatt tcctctcgcg tcgtgacgcc cgaagtcaac gaaattgtgg
    10861 tgaacccgaa tgcgaccctc gactggcaac tggcactacg ccaggcagca ggaaaaaccg
    10921 atttagcgcg cgatatgctg caaatgttac tcgatttcct gcctgaagtt cgcaacaaag
    10981 ttgaggaaca gctggttgga gaaaacccgg aaggcctggt tgatttgatt cataaactgc
    11041 atggcagttg cggctatagc ggtgtgccgc gtatgaagaa tctctgccaa ctgatcgaac
    11101 aacagctacg tagcggtact aaagaagaag atttggagcc ggagctgctg gaactgttgg
    11161 acgagatgga taatgtcgcg cgcgaagcca gcaaaattct cgggtaacgg acgtcttgtc
    11221 gagtagcaat cggcttttta tgacgctggt ttcagattgg tgacaaagtg cctgtgttta
    11281 tgccggatgc ggcgttaacg ccttatccgg cctacaaaac caattaaatt caatgaatta
    11341 aaaaatgatg tagacctgat acatagcaca acaggctatt ttgcgtttag catcagtctc
    11401 aagccggctc cagatagagc cggttttggt tttctgtctt aacgcaccat gcacgggcgc
    11461 ttgttatcga acgtccagcc aggaatcaga tactgcattc ccatcgcatt gtcacgcgcg
    11521 caaagcccgt gtttctgata cagctcatgg gctttcatca cttgatccat atcgatttct
    11581 acacccagcc ccggtttttc cggcacctga accagaccgc ctttgatctc aaacggttct
    11641 ttggtcaagc gctgattgcc ttcctgccaa atccagtgcg tatcaatcgc ggtgatttta
    11701 cccggtgcag cggcggcaac atgggtaaac atcgccaggg aaatatcgaa gtggttgttt
    11761 gagtgtgaac cccaggtcag gccaaattca tggcacattt gcgccacacg taccgaacct
    11821 tgcatcgtcc agaaatgcgg atccgccagc gggatatcaa cggattgcag ggagagcgta
    11881 tggcccatct gccgccagtc ggtggcgatc atattggttg cggtcggcag gccagttgcg
    11941 cgacggaact ctgccatcac ttcacgcccg gagaaacctt gctccgcacc acacggatct
    12001 tctgcataag ccagcgaccc tttcaggtat ttaccaattt taatcgcttc gttcagcgac
    12061 caggcaccgt taggatcgag cgtaatacgc gcctgcggga agcgttgcgc cagtgccaca
    12121 atagactcgg cctcttcttc cccggccagt acaccgcctt tcagtttgaa atcgttgaag
    12181 ccatattttt catacgccgc ttccgccagg cgcaccaccg catccggcgt catcgcttct
    12241 tcatgacgca ggcgatacca gtcgcatgag tcatccggct ggctttgata cggcagcggt
    12301 gtggctttgc gatcaccgac gaagaacaga taaccgagca tttcgacttc gctacgctgt
    12361 tgaccatcgc ccagcaggga tgccacgttt acccccagat gctgccccag cagatccagc
    12421 attgccgctt ctatcccggt aactacatga atagtggtac gcaaatcaaa tgtctgcaaa
    12481 ccgcgcccac cagcatcacg atcggcaaaa gtattacgca ccagcgtcag aacgtttttg
    12541 tattcaccca gcgttttacg gattttctcg ccgccgggaa tttcccctac gccagtgtga
    12601 ccagaattat ctttgataat cataatatta cgcgtaaaga acggtgcgtg tgcaccactc
    12661 agattcatca gcatactgtc atgacccgcc accgggatga cctgcatttc agtaacaaca
    12721 ggcgtcgtaa attgagaact catatttatg tcctttttca gaatcaatga cggccgaaaa
    12781 cgggacgttt acggtcaaag gtccagccgg ggatcaggta ctgcatcgga cctgcgtcgt
    12841 tacgcgcacc gccaggcaga cgtttatagg cctcatgtgc cttttgtacc tgttcccagt
    12901 ccagttccac gcccagaccg ggcgcatcag gaacggcaat ttttccgttt ttaatctcca
    12961 gcggattttt ggtcaggcga caatcgccct cctgccaaat ccagtgggta tcgatagcgg
    13021 taggattacc cggtgccgcc gcgcccacat gggtaaacat cgccagagag atatcgaaat
    13081 ggttattaga atggcagccc caggtcagcc cccagtcgtc gcaaagctgc gccacacgga
    13141 ctgcaccgga aagcgtccag aagtgcggat cggcaagtgg aatatctacc gcattgagca
    13201 tcaccgcatg acccatttcg cgccagttgg tggcgatcat gttagtcgcg acgggcaagc
    13261 cggtcgcccg ccgaaactcc gccataactt cacgaccgga gaaaccctgt tctgcgccgc
    13321 atggatcttc ggcataggta agaacatcat tcagcccttt gcatagagaa atggcttcat
    13381 caagtagcca ggcaccgttg ggatcaacgg taatccgcgc atcagggaag cgtttcttca
    13441 atgcacgaac agtgtcgatt tcttgctcgc caggtaacac cccgccctta agtttgaaat
    13501 ctttaaagcc atagcgatcc tgtgaggctt ccgccagacg cacaacggct tcgctgttca
    13561 tcgctttctg atggcgcaac tgataccact catggttgcc cggcgtattt tccagataag
    13621 gaagatcggt tttggtccga tcaccgatat aaaacagata accgaggacg gtaatagtct
    13681 cgcgttgctt gcctggcccc aacagttcgc agaccggaac attcagcgcc ttacctagca
    13741 ggtcaagcaa ggcggcttcc agcgccgcca cggcattaac gtgcaattca aaagtccagg
    13801 cacctttgcc gaaggtatca aaatcggctg cctgattacc tttatgcacc tgctgaacca
    13861 ctttattcag gcgcgcaact tcctggccca gaaccatcgg aatagcatcg accagtgttt
    13921 gataaatcac ctctccgccc ggcgcttcac caatgccggt atgcccggcg ttatcggtga
    13981 gtaccacaat attgcgggtg aaatatgcgt tatgtgcccc accaatatta agcaacatgc
    14041 tatcgtgccc ggcgaccgga atgactttca tatcagtaat aacaggactg gattgtgtcg
    14101 tcatcttatt gccccgcgac aggtttcagc tcgatacgct tgatatcgcc caccagcacc
    14161 aggtagctca gtaccgcgat taaggcatga acaccaacat aaatcagcgc cccattaaag
    14221 gagccagtcg tgccaacgat ataaccaatt gcgattggcg tgacgatgcc agaaatgtta
    14281 ccgaacatgt tgaacaggcc accggaaaga ccgctgatct cttttggcgc ggtatctgcc
    14341 attactgccc aacccagcgc cccgatgccc ttaccgaaga aggccagcgc cataaagccg
    14401 atgatcatcc actcaacgtt gacgtagttg cagaacacca tcaccatcga cagcaacatg
    14461 cccattacga tcggtgtttt acgcgcaatg ttcagcgatc ccgtgcggcg catcagccag
    14521 tcggaaataa tcccacccag cacaccgccg ataaaaccgc aaaccgccgg aacggaagcc
    14581 acaaagcccg ctttcagaat tgacatccca cgcgcctgca ccagataaac cggaaaccag
    14641 gtaatgaaaa agtaagtcag ggcgttgata cagtactgac cgatataaac gccgatcatc
    14701 atccgtgacc cgagcagttg tttgatctgc ccccacttca cgctgaacgg cactttaact
    14761 ttggtgtttt gctgatccat attgatcagc gcgccacccg cggcgatgta ctccagctct
    14821 ttctgattta cccccggatg ttgatttggc tcgtggatga ctttcaacca gataaagctg
    14881 atgacaatcc ccagaccgcc cataaagaag aagacgtgtg accagcccac ttcatgcgtc
    14941 agccagccca taatcggcgc gaagatcacc gttgcgaagt attgagcgga gttaaaaatc
    15001 gacaccgccg ttcccctttc ctgcgccgga aaccaagccg caacaatgcg actgttgccg
    15061 gggaaagatg gcgcttcagc aagcccgacc aggaagcgca gagtaaacag ggcaacgata
    15121 atgccgaatc cactaaagat atcgacgaag ccttgcagca aggtaaacat cgaccagata
    15181 aagatcgacc agaagtagac gcgttttgaa ccaaaacgat ccagcaacca accaccaggg
    15241 atctgcccga taacataagc ccatgagaag gcagagaaca catagcccat tcccacggga
    15301 tcaaggccga tatctttggc catttccgaa ccggcaatag aaagcgtagc gcggtcgccg
    15361 tagttgaagg atgtgacgat aaacaacatc accactatcc agtaacgagc attcgtgcgt
    15421 ttttccacac tgctcgcagc ctgacttaaa gaactcgttg ttgcactcct gaaaattcgc
    15481 gttagccacg ctaactctgg actgcgacat agccaggaaa tcagaggtga cgtagggtgt
    15541 tttttgccgt ttttataggt cgttcgccga atacggcgcg tgtttatatc tggcaatagc
    15601 agtataaaaa gcgcgccata gcggctcacc gtgcaacaac acaacattaa tgcgttcaat
    15661 gaggcccgat tttggcatta gccctggacg gtggaatcca cttcacggaa atgaaaacaa
    15721 gaacaagaaa ggaagggtta aaacgaagaa ataaagaaga gtatgaaatg gatcgcttga
    15781 ctccaggcaa acgccagtaa aaatccgcgc tatgaagcag tttttactgg catttgcctg
    15841 aaaagattcg attcagcacc gctaaaacga catttaccgc tcgctgaaca tatcaggaca
    15901 acagcgtgcc ccactgttcg acccacggat ttgattccgt ttccggttcc aggttctcac
    15961 tggcgtcaat caacagcatt tcgccaactc gctgagcgct ctgttcctgc aacaaggcat
    16021 cgaactgttt gccgccattg cagaaattta cataactgct gtcgccgagc gcaatcacgc
    16081 cataacacag attcggctgg aagcccagac tatctttgat tccctgaaag agcggcacaa
    16141 tgctatcagg aaggtcgccc tgcccggtcg tggacgtaac caccagaaca tacttatcct
    16201 gataaggcag ccagtcgctt aattcaggat cttcaaatac cgttgctttg tggccctgtg
    16261 cggtcagaat tgcttctgct tcttccgcca ctaacagcga atttccgtac atggtgccga
    16321 caaaaatacc aatttccgcc ataccgtttt ctccctggat taggaactta tctctccatc
    16381 ctgacccgat ggcgcactga actcaaccct ttcattttca ggaagcagac cgcgccagcc
    16441 aaattgtgat aacgcctgca tccaggtgtc gtccaggccc gcgtgaatag tcagcggctc
    16501 gccagtaaaa ggatgcgtca atgacaactg actggcatgt agcattaacc gctggaggcc
    16561 aaaatgctca gcaccgctgc gattctggcg taaatcgcca tgtttgctat cgccaataat
    16621 cggatgacgc aaatgggcaa gatgtcggcg aagctgatgt ttgcgtccgg ttttcggctc
    16681 cagttccacc aggccgtagc gcgtggtcgg gtaacgtccg gtcgctaccg gcatttctac
    16741 ggtcgccaga ccgcgataat gcgtcactgc tggctgcggg cctttatctt cgcgggcaaa
    16801 tttatcagcg attttgtcca gttcttccac cagtggataa tccagcaccg cttcttccat
    16861 caaccagccg cgcacaatcg catggtaacg tttctggatt tggtgctgtt caaactgttg
    16921 tgccagcagc cgtccggcct cgctggataa tcccatcaac aacacaccag aagtgggtcg
    16981 gtccagacga tgagcagtaa aaacatgctg tcctatctgg ttacgcacgg tttgcatgac
    17041 cactactttc tcgtcgcgat ccagccagct acggtgaacc agccagccgg agggtttatt
    17101 taccgaaacc agccattcat cctgatagag tatttccagc attagctcgc atcatccgca
    17161 aaaagagcat ccagtttttc caactcagcc agaataagcg cgtgttgcgg atggtccgtc
    17221 gccagcgcca tttcataata gggtgcaaca gcaaaagcgc ccggtaacgg ctggttgtta
    17281 tttaacaaat cgtgcattcg cgggatcagc acccactgca accactccag tggttccatg
    17341 gtgtccataa agaacggttg ggtactatta aattgatgcg gctggggttc atcgtttcgc
    17401 cagtgctgat gttcacgcag taacgcttca agcgcctgca actggagacg aacgcggtca
    17461 tgagtggtca taaagcaacc tcaataagaa aaactgccgc gaaggatagc aggaataaaa
    17521 aaagggagca ctgtattcac agcgctcccg gttcgtttcg cagcattcca gctacttttg
    17581 ttgctccctg ctcatccttg acaacttttc ctctggcctt gcggccaatc gttcatcctg
    17641 aactattgct tcctgctcac accaccccga tgtgatactt catcctgaag tgtccttggc
    17701 cttcctgacc agccgaatca tcctgaccgg ttctcattct ccatcctgga ggtgtccttt
    17761 aacgcgtcct gcgtcatcct cttcgcttca tccagaagcc tttccctgaa acaccatcct
    17821 ggtgtgtcct gcagaagtgt catcatcctg atgttcactt cgttgtctga ctccctgtcg
    17881 acgaagatag aatcgtcttt ttcgcgaagt cttacaaggc gcttacagac aatgccttaa
    17941 ggaaaatttc atatgaaagt taagaattaa caatcaacat tttgatttta aatgaaaata
    18001 acgattctta tctttttaag tattcgctta tttcctggcg atctcctaca gagtctgtaa
    18061 gagatctctc acaaccagat ggggtatttg gattacagaa gaggcttaag ttgattaagg
    18121 aattccgcaa gatttgaggc taaatgcgtc cgtttacgtg ttcccagcgt ctctttgcac
    18181 acttcaccgg agagattaca caccgagatg acctctaact cttcttccag cgtcgcgata
    18241 aatagcgtgg gcggcagttt tagccttttc tgagtcacca gatgaccaat cagattttcc
    18301 tgcacccgac ggaagtcgtc ttcactccag gtttgcagca atgtcagttt gatatcgcca
    18361 aactgcgcgt gcatatcccc ggcaaactga gtcgtataaa aggtatgaat tgtgggttgt
    18421 atcacaatat caaaagcgcg ttcaaccgcg tttacatttt gttccccggt aaacggctgg
    18481 ggttgccagt atacggcatc ttcggtagtt gaaataatgc acggtgaagg aacaccgtac
    18541 agttcctcac ttaacggcca gcttttatgc tcttcatgcc acgcatcgca gtagcgtgca
    18601 gtaaaatctt tcagggcttg tgcggtcaaa tcgtccacta atttctctct tcacttaaac
    18661 cagatacact tgtcgtttag tttatctggt ttatgacggt gaaacatgtc ttcttatgca
    18721 aaccatcagg cacttgcggg cctgactctt ggaaaatcaa ccgattaccg ggatacctat
    18781 gacgccagcc tactgcaagg cgttccacgc agcctgaatc gcgacccgct gggtctgaaa
    18841 gcggataacc tgccttttca cggtacggat atctggacgc tgtatgaact ttcctggctg
    18901 aatgcgaaag gtttgccgca ggtcgctgtc ggtcatgttg aacttgatta caccagcgta
    18961 aatctgattg agtcgaagag ttttaagctc tatctcaaca gttttaacca gacgcgtttt
    19021 aataactggg atgaggtgcg ccagacgctg gagcgcgact taagcacttg cgctcagggt
    19081 aagattagcg tggcgttata tcgtcttgat gaactggaag gccagccgat aggtcatttt
    19141 aatggcactt gcattgatga ccaggatatc actatcgata actatgaatt cactactgac
    19201 tatctggaga atgccacctg tggtgaaaaa gtagtggaag agacgcttgt cagccacctg
    19261 ctgaaatcaa actgcctgat cacccatcaa ccagattggg gttcgatcca aattcagtat
    19321 cgtggacgcc aaattgacag agaaaaactg ctgcgttacc tggtctcatt ccgtcatcac
    19381 aacgagttcc acgaacagtg cgtggaacgc atctttaatg acctgttacg cttctgccag
    19441 ccagaaaaat tgagcgttta cgcacgttat acccgtcgtg gcggtctgga cattaacccg
    19501 tggcgcagta atagcgattt tgtcccatcg accacaagac tggttcggca ataaattttt
    19561 tctcaatttt gcgtgctgga ttcacgcaga aggttgtgaa aggtcatcag gcagggctat
    19621 tgtaatcaaa gggaatgacg atattcgtcc cataaggagt ttttcttgat tacacatatt
    19681 agcccgcttg gctccatgga tatgttgtcg cagctggaag tggatatgct taaacgcacc
    19741 gccagcagcg acctctatca actgtttcgc aactgttcac ttgccgtact gaactccggt
    19801 agtttgaccg ataacagcaa agaattgctg tctcgttttg aaaatttcga tattaacgtc
    19861 ttgcgccgtg aacgcggcgt aaagctggaa ctgattaatc ccccggaaga ggcttttgtc
    19921 gatgggcgaa ttattcgcgc tttgcaggcc aacttgttcg cggtcctgcg tgacattctc
    19981 ttcgtttacg ggcaaatcca taacaccgtt cgttttccca acctgaatct cgacaactcc
    20041 gtccacatca ctaacctggt cttttccatc ttgcgtaacg ctcgcgcgct gcatgtgggt
    20101 gaagcgccaa atatggtggt ctgctggggc ggtcactcaa ttaacgaaaa cgagtatttg
    20161 tatgcccgtc gcgtcggaaa ccagctgggc ctgcgtgagc tgaatatctg caccggctgt
    20221 ggtccgggag cgatggaagc gccgatgaaa ggtgctgcgg tcggacacgc gcagcagcgt
    20281 tacaaagaca gtcgttttat tggtatgaca gagccgtcga ttatcgccgc tgaaccgcct
    20341 aacccgctgg tcaacgaatt gatcatcatg ccagatatcg aaaaacgtct ggaagcgttt
    20401 gtccgtatcg ctcacggtat cattatcttc cctggcggtg tgggtacggc agaagagttg
    20461 ctgtatttgc tgggaatttt aatgaacccg gccaacaaag atcaggtttt accattgatc
    20521 ctcaccggcc cgaaagagag cgccgactac ttccgcgtac tggacgagtt tgtcgtgcat
    20581 acgctgggtg aaaacgcgcg ccgccattac cgcatcatca ttgatgacgc cgctgaagtc
    20641 gctcgtcaga tgaaaaaatc gatgccgctg gtgaaagaaa atcgccgcga cacaggcgat
    20701 gcctacagct ttaactggtc aatgcgcatt gcgccagatt tgcaaatgcc gtttgagccg
    20761 tctcacgaga atatggctaa tctgaagctt tacccggatc aacccgttga agtgctagct
    20821 gccgacctgc gccgtgcgtt ctccggtatt gtggcgggta acgtaaaaga agtcggtatt
    20881 cgcgccattg aagagtttgg tccttacaaa atcaacggcg ataaagagat tatgcgtcgt
    20941 atggacgacc tgctacaggg ttttgttgcc cagcatcgta tgaagttgcc aggctcagcc
    21001 tacatccctt gctacgaaat ctgcacgtaa tctcccctcc cccggtgacg tttgttgccg
    21061 ggttttctcg tttttggtca cttactcatc aactcatttc atttgttata tgaatgtttc
    21121 ttaccaccct cacgcgacaa atatcatcac agttaatatg tcatacaatt tatgttgcaa
    21181 cgcaaacgtt tccctatttt cataaaccgt attttatcgc tatgaaaaag aaattatcgc
    21241 catgattaac taaaagtatt gattttttca gttcaaccta catatattgc gcgccctgga
    21301 agaagtcaga tgtcgtttaa tgggcaaata ttgcccttaa attctctttt acttttgatt
    21361 tacagagtaa agcgttggga taatctatct tccaagtaga ttattgtatt tgagatcaag
    21421 atcactgata gatacataac ttgtgtgtat ctttccgccc tcaaattatt acggcggtaa
    21481 atgattaagc catcgccgat agacagattt catttttacg gtcaggcacc ttcccgggct
    21541 gaactggcta aaagctgaat tatttgcatt cctccaggag aaatagatgg aaacgactca
    21601 aaccagcacg attgcgtcga aagactctcg tagtgcctgg cgcaagacag acaccatgtg
    21661 gatgctgggc ctttacggca cggcaatcgg cgcgggcgtg ctgttcctgc caatcaacgc
    21721 cggtgttggc ggtatgatcc cgctgatcat catggctatc cttgcgttcc cgatgacgtt
    21781 ttttgctcac cgcggcctga ctcgcttcgt actgtctggt aaaaacccgg gcgaagacat
    21841 caccgaggtt gtagaagaac actttggtat tggcgcaggt aaactgatta ccctgctcta
    21901 cttcttcgct atctacccga tcctgctggt ttatagcgtg gcaatcacca atacagttga
    21961 aagcttcatg tctcaccagc tgggtatgac gccaccgccg cgtgcgattc tgtcgctgat
    22021 cctgatcgtg ggtatgatga ccatcgttcg cttcggtgag cagatgatcg ttaaagcgat
    22081 gagtattctg gtgttcccgt ttgttggcgt attgatgctg ctggctctgt acctgatccc
    22141 gcagtggaac ggcgcagcac tggaaacgct gtctctggac actgcatctg caaccggaaa
    22201 cggtctgtgg atgaccctgt ggctggcaat tccggtaatg gtgttctcgt tcaaccactc
    22261 tccgatcatc tcttctttcg ccgttgcgaa gcgtgaagag tacggcgata tggcagaaca
    22321 gaaatgctcg aagatcctgg cattcgcaca catcatgatg gtgctgaccg taatgttctt
    22381 cgtcttcagc tgtgtactga gcctgactcc ggcagacctg gctgcggcta aagagcagaa
    22441 catctcgatt ctgtcttacc tggctaacca ctttaacgca ccggttatcg cgtggatggc
    22501 tccgattatc gcgattatcg ctatcaccaa atccttcctc ggccactacc tgggcgcacg
    22561 tgaaggcttc aacggtatgg tgattaaatc tctgcgtggt aaaggtaagt ctatcgaaat
    22621 caacaagctg aaccgtatca ctgcgctgtt catgctggta acgacctgga ttgttgccac
    22681 cctgaacccg agcatcctgg gtatgattga aaccctgggc ggtccaatca tcgcgatgat
    22741 cctgttcctg atgccgatgt acgcaattca gaaagtaccg gcaatgcgta agtacagcgg
    22801 tcacatcagc aacgtattcg ttgtcgtgat gggtctgatt gcaatctccg caatcttcta
    22861 ctctctgttc agctaagtcc tttcgcgccg ctttcgggcg gcgcttcctc cgttttaacg
    22921 cgatgtattt cctatgatta gcgtattcga tattttcaaa atcggcattg gcccttccag
    22981 ttctcatacc gttggaccaa tgaaagcggg taaacaattt accgacgatc tgattgcccg
    23041 taacctgctt aaagacgtga cccgcgtggt ggttgacgtg tacggctcgc tctctctgac
    23101 cggtaaaggc caccacactg atatcgccat tattatgggc ctggcgggta acctgccgga
    23161 taccgtggat atcgattcca tccctggttt tattcaggat gtgaatactc atggtcgcct
    23221 gatgctggca aacggtcagc atgaagtgga gttcccggtt gatcagtgca tgaacttcca
    23281 cgccgacaac ctttctctgc atgaaaacgg tatgcgcatt accgcgctgg cgggcgataa
    23341 agtcgtttac agccagactt actactctat tggcggtggc tttatcgttg atgaagagca
    23401 ttttggccag caggatagcg caccggttga agttccttat ccgtacagtt cagcagccga
    23461 tctgcaaaaa cattgtcagg aaaccgggct gtcactctct ggcctgatga tgaaaaacga
    23521 gctagcgctg cacagcaaag aagagctgga acagcacctg gcgaacgtat gggaagtcat
    23581 gcgtggcggt attgagcgcg gtatttccac cgaaggcgtg ttgcctggca aactgcgcgt
    23641 tccacgccgt gctgcggcac tacgccggat gctggtcagc caggataaaa ccaccactga
    23701 cccgatggcg gttgttgact ggatcaacat gtttgcactg gcagtgaacg aagagaacgc
    23761 tgctggcgga cgcgtggtga ctgcgccgac taacggtgcg tgcgggatta tcccggcagt
    23821 gctggcgtac tacgacaagt ttatccgcga agtgaacgct aactcactgg ctcgttacct
    23881 gctggtagcc agtgccattg gttctcttta taagatgaac gcgtcaattt ctggtgctga
    23941 agtcggctgc cagggtgaag ttggtgtggc gtgctcaatg gcggcggctg gtctggcaga
    24001 actgttaggt gcaagcccgg cgcaggtgtg catcgcggcg gaaatcgcca tggagcacaa
    24061 cctcggtctg acgtgtgacc cggtcgccgg acaggtccag gtgccatgca tcgagcgtaa
    24121 cgccattgcg gcagtaaaag cggtgaacgc cgcacgtatg gcgctacgcc gtaccagcga
    24181 gccgcgcgtc tgcctcgata aagttatcga aaccatgtac gaaacaggta aagatatgaa
    24241 cgccaagtac cgcgaaacct ctcgcggcgg cctggcaatg aagatcgttg cctgcgatta
    24301 atcgctctcc agaggcctcg ttttgcgagg cctcttcccg atttctcatc cagccgtagc
    24361 ctgttccggc atcgaatgtt acccttatcg cctgatcttt aagggggtta tcgtggctgt
    24421 tcatttgctt attgtcgatg cactgaatct tattcgtcgc attcatgccg ttcagggatc
    24481 gccctgtgtc gaaacctgcc agcatgcgct cgatcagctc attatgcaca gccagccaac
    24541 ccacgcggtc gccgtttttg atgatgaaaa ccgcagtagc ggctggcgtc atcagcgttt
    24601 accagattac aaagcgggtc gaccgccaat gccggaagag ttgcacgacg agatgcctgc
    24661 attacgcgcc gcctttgagc aacgcggcgt cccgtgctgg tcaaccagcg gcaacgaagc
    24721 cgatgactta gccgccacgc tggcggtcaa agtgacacag gccgggcatc aggcaacgat
    24781 tgtttcgaca gataaaggct actgtcagtt actttcaccg acattacgta ttcgtgatta
    24841 cttccagaaa cgttggctgg atgcgccatt tatcgataaa gaatttggcg ttcaaccgca
    24901 gcagttgccc gattactggg gacttgcggg gatcagcagt tcaaaggtac cgggtgttgc
    24961 gggaatcgga ccaaaaagcg ccacgcagct gctggtcgag tttcagagtc tggaagggat
    25021 atatgagaat ctggatgcgg ttgccgaaaa gtggcgcaaa aaattagaaa cccataaaga
    25081 gatggcgttt ctgtgccgcg atattgcccg cttacaaacc gatttgcata tcgacggcaa
    25141 tttacagcaa ttgcggttgg tacggtaacg gcgagccgga tgcaaacgat tatcaacaca
    25201 ttgaatttaa tgcacttata taccggataa gacgcggcta gcgtcgtatc cggcattatc
    25261 acatcagcgc atttaccagg cggtatggta aagctctaca atatcctcaa gcgttgcttc
    25321 acgcgggttg ccaccggtac aaacatcatc cagtgccgcc tgcgccagtg ccggaatgtc
    25381 ttccttgcgt acaccaacat cacgcaaatg tggcggaata ccgacatcac ggttgagagc
    25441 aaacaccgct tcaacagcgg cattacgcgc ctcttccagc cactttcacg cccataacgc
    25501 gcgcgatatc gcggtacttc tcaccggtaa agtcagcgtt atagcgcatg acatgcggta
    25561 acaggatggc gttcgcaaca ccgtgtggag tgttgtaaaa cgcgcccagt ggatgcgcca
    25621 taccatgcac caaccctaac ccaacattcg agaagcccat acccgcaaca tactgcccga
    25681 gcgccatttc ttctccggca tccttatcac cagcaaccga tcctcgcagc gccccagcaa
    25741 tgatttcaat cgctttaatg tgcagtgcat cggttagcgc ccacgcgcca cgggtaatat
    25801 acccctcaat agcatgagtg agcgcatcga cacccgtcgc agctttcagc gctggaggca
    25861 taccatccat catgtcagcg tcaataaacg ccacctgcgg gatatcatgc ggatcaacgc
    25921 aaacaaactt gcgccgtttc tcttcgtcag tgatcacgta gttaatggtc acttctgccg
    25981 cagtacctgc tgtggtagga attgccagaa tcggtacact gggtttattg gtcggggaaa
    26041 gcccttccag gctacgcaca tcggcaaact ccgggttgtt gctgataatg ccaatcgctt
    26101 tacaagtatc ctgtggagaa ccaccaccaa tagcgatcag gtaatccgcg ccgctattct
    26161 ggaatacacc gagcccttct ttgacgacag taattgttgg gttgggcact acgccgtcgt
    26221 aaatcgccca tgccagccct gcagcatcca tcttatcggt cactttcgcc accacgccgc
    26281 attgcaccag cgttttatcg gtgacgatca gcgccttctg ataaccacgg cgtttcacct
    26341 catcggttaa agccccaaca gcaccccgac caaaccatgc cgtttcgttc agaatcattc
    26401 tgttagccat catccttctc cttgttgctt tacgaaatta ctcttcaatc cgtaacccat
    26461 aggttttgaa tttctccagc actacggcaa tctcttcatc gctcagcact ggcaccgggt
    26521 ccgtaatcgc cagggtcgtc aggtaaagtt gcgccagcac ttcaacttca tgcgccagcc
    26581 ataacgcttt ttccagattc acctcacaag cgataagccc atgatgttgt aacaaagttg
    26641 ccttacgatt tttgagagcc agcgcaacat gttcagaaag ttcgcgtgtt ccaaaggtcg
    26701 cataaggcgc gcaaggaata gaattaccac ctgccgccgc aatcatgtag tgaatagcgg
    26761 ggatcggtcg gttaagaatg gaaactgctg tgcaatgaac ggcatgattg tgaacaaccg
    26821 cgttggcatc cggtctgctt tgataggccg ccatatggaa acgccattcg cttgagggga
    26881 gctttccttc ctcatgttta ccgttaccat caataaaggc aatatgcgac tccgtcagtt
    26941 tttcatatgg aatgcctgta ggcgtaatca gcatcccatc ctgataacgt acactgacgt
    27001 tccccgctgt cccctggttc agtcccaggc gggtcatttc caggcaagtg tcaataatct
    27061 gacgagcaag tttatttcgt tccattagct acctctctct gattcaaaac agggcaataa
    27121 tgttgttcct ttcacactat tgaattagcc gtttaattac ccaccat
//
LOCUS       DADLFA010000038        25805 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000038 DADLFA010000000
VERSION     DADLFA010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25805
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.038"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(82..2301)
                     /locus_tag="JQH65_001362"
     CDS             complement(82..2301)
                     /locus_tag="JQH65_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HAY6138831.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSSEEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVLHPAYGNVRIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(2412..3824)
                     /gene="ftsP"
                     /locus_tag="JQH65_001363"
     CDS             complement(2412..3824)
                     /gene="ftsP"
                     /locus_tag="JQH65_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HAY6138832.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDSRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(3899..4636)
                     /gene="plsC"
                     /locus_tag="JQH65_001364"
                     /gene_synonym="parF"
     CDS             complement(3899..4636)
                     /gene="plsC"
                     /locus_tag="JQH65_001364"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HAY6138833.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(4869..7127)
                     /gene="parC"
                     /locus_tag="JQH65_001365"
     CDS             complement(4869..7127)
                     /gene="parC"
                     /locus_tag="JQH65_001365"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001639862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAY6138834.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LVSERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(7673..8155)
                     /locus_tag="JQH65_001366"
     CDS             complement(7673..8155)
                     /locus_tag="JQH65_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY6138835.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPKAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTVWGIIRHWLPASGEKMRKAPILFHYTNLAEGMTEQRLETDVYVPLA
                     "
     gene            complement(8208..8600)
                     /gene="ygiW"
                     /locus_tag="JQH65_001367"
     CDS             complement(8208..8600)
                     /gene="ygiW"
                     /locus_tag="JQH65_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HAY6138836.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            8752..9411
                     /gene="qseB"
                     /locus_tag="JQH65_001368"
     CDS             8752..9411
                     /gene="qseB"
                     /locus_tag="JQH65_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HAY6138837.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLASEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            9408..10757
                     /gene="qseC"
                     /locus_tag="JQH65_001369"
     CDS             9408..10757
                     /gene="qseC"
                     /locus_tag="JQH65_001369"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HAY6138838.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDS
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNVQGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPSGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            10867..11448
                     /gene="mdaB"
                     /locus_tag="JQH65_001370"
     CDS             10867..11448
                     /gene="mdaB"
                     /locus_tag="JQH65_001370"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HAY6138839.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            11479..11793
                     /locus_tag="JQH65_001371"
     CDS             11479..11793
                     /locus_tag="JQH65_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAY6138840.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(11841..13733)
                     /gene="parE"
                     /locus_tag="JQH65_001372"
     CDS             complement(11841..13733)
                     /gene="parE"
                     /locus_tag="JQH65_001372"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAY6138841.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQIYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(13762..14343)
                     /gene="yqiA"
                     /locus_tag="JQH65_001373"
     CDS             complement(13762..14343)
                     /gene="yqiA"
                     /locus_tag="JQH65_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HAY6138842.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(14343..15170)
                     /gene="cpdA"
                     /locus_tag="JQH65_001374"
     CDS             complement(14343..15170)
                     /gene="cpdA"
                     /locus_tag="JQH65_001374"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HAY6138843.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(15195..15617)
                     /locus_tag="JQH65_001375"
     CDS             complement(15195..15617)
                     /locus_tag="JQH65_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HAY6138844.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTTVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(15618..16247)
                     /gene="nudF"
                     /locus_tag="JQH65_001376"
     CDS             complement(15618..16247)
                     /gene="nudF"
                     /locus_tag="JQH65_001376"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HAY6138845.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHSLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            16452..17933
                     /gene="tolC"
                     /locus_tag="JQH65_001377"
     CDS             16452..17933
                     /gene="tolC"
                     /locus_tag="JQH65_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HAY6138846.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSIVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            18081..18752
                     /locus_tag="JQH65_001378"
     CDS             18081..18752
                     /locus_tag="JQH65_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HAY6138847.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLASCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            18758..19918
                     /locus_tag="JQH65_001379"
     CDS             18758..19918
                     /locus_tag="JQH65_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HAY6138848.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLPLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(19956..20771)
                     /gene="ygiD"
                     /locus_tag="JQH65_001380"
     CDS             complement(19956..20771)
                     /gene="ygiD"
                     /locus_tag="JQH65_001380"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311954.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HAY6138849.1"
                     /translation="MTPLVKDIIMSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMT
                     LPRPQAIVVVSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLV
                     ELLAPVPVTLDKEARGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLA
                     ALRDEGIMLVASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVN
                     YLDHEGGALSNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            20887..21648
                     /gene="zupT"
                     /locus_tag="JQH65_001381"
     CDS             20887..21648
                     /gene="zupT"
                     /locus_tag="JQH65_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HAY6138850.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGISPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAVAGIMVALSVDELMP
                     LAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(21706..21876)
                     /locus_tag="JQH65_001382"
     CDS             complement(21706..21876)
                     /locus_tag="JQH65_001382"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6138851.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(22136..22789)
                     /gene="ribB"
                     /locus_tag="JQH65_001383"
     CDS             complement(22136..22789)
                     /gene="ribB"
                     /locus_tag="JQH65_001383"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HAY6138852.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(22893..23041)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            23103..23453
                     /gene="ubiK"
                     /locus_tag="JQH65_001384"
     CDS             23103..23453
                     /gene="ubiK"
                     /locus_tag="JQH65_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HAY6138853.1"
                     /translation="MTYPYRTTMVLNTYQYRETTMIDPKKIEQIARQVHESMPKGIRE
                     FGEDVEKKIRQTLQAQLTRLDLVSREEFDVQTQVLLRTREKLALLEQRMSELENRSTE
                     IKKQPDPETLPPTL"
     gene            23737..24288
                     /locus_tag="JQH65_001385"
     CDS             23737..24288
                     /locus_tag="JQH65_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HAY6138854.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILNSQSGEVTLGTPVVITFNNTNSYQELNFKVRM
                     ESPGKDATPGNVYAQADYKIAYE"
     gene            24348..>25805
                     /locus_tag="JQH65_001386"
     CDS             24348..>25805
                     /locus_tag="JQH65_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY6138855.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLNAIINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEEAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQGQLDVKVEEE
                     DGQVTQWQVASNSIPYLTRKGQIRYTTAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNV
                     SLHGGSVLTNRDYQSLAAGVGFNLNSLGSLSFDVTRSDTQLHNQDKETGYSYRANYSK
                     RFESTGSQLTFAGYRFSDKNFVTMNEYINDTNHYTN"
BASE COUNT         6459 a         6802 c         6372 g         6172 t
ORIGIN      
        1 tcggcatata aataaatgcc taatgagata gattctgatg taatatcccc ggtagcaatt
       61 ctgctaccgg gaacagcgaa tttaacgtcc aaccgccgct ttaggccgtt tcttcgcacc
      121 agcattcacc ggacgagatt gcgtagacga cgcttttttt gccgtagcag gcgtctgacg
      181 ctgggtcgcc atcggcgtat gtttcgtcaa cgccggacgg gtattgcggt tctggcgacg
      241 agcttcacgc atctcttcaa tggttggcgc aggcactaag caatcgcgac ggctaccaat
      301 cagatgcttt ttacccatcg cttccagcgc ctggcggatt aacggccagt ttgccggatc
      361 gtggtaacgc aacaacgctt tatgcaaacg acgctgcttg tcgcccttcg gtacgaagac
      421 gtcttcactc ttataaccaa tcttcgccag cggttttttc ccggtgtaat acatggtggt
      481 tgagttcgcc agcggcgatg ggtagaagtt ctgtacctgg tcgagacgga aacgatgctt
      541 tttcagccac agcgccagat tcaccatatc ttcatcacgc gtaccggggt gcgcggagat
      601 gaaatagggg atcagatact gctctttacc tgcctgtttt gagtaagtat cgaacagctc
      661 tttaaagcgg tcatagctgc ccatgcccgg cttcatcatc ttcgataacg gcccttcttc
      721 ggtatgttcc ggggcaatct tcagataacc gccgacgtga tgggtcgcca gttctttgat
      781 atagcgcgga tcttccacgg cgatgtcata acgcacacca gaggcaatca ggatcttttt
      841 aatgcctttc agatcacgcg cacggcgata gaggttgatc gttggttcgt ggttcgtgtc
      901 catgtgcgga caaatatccg gataaacgca cgacaaacgg cggcaggttt gctcagcgcg
      961 tggcgatttg cagcgcaaca tatacatgtt ggcagttggc ccaccgagat cggaaattac
     1021 gcccgtaaaa cctggaacgg tgtcgcggat cgcttcgatc tcattaatga tcgaatcttc
     1081 ggaacggctc tgaataatgc gcccttcgtg ctcggtgata gaacagaaag agcagccgcc
     1141 aaagcagcca cgcataatgt tgaccgaaaa acggatcatt tcgtaagccg gaatacggac
     1201 attgccatag gccggatgta gcacgcgctt gtatggcagc gcaaaaacgc tgtccatctc
     1261 ttcggtagaa agcggaatag caggcgggtt gatccacaca tagcggtcgc cgtgtttttg
     1321 catcaatgcg cgggcacagc ctgggttggt ttcgtggtgc agaatacgcg aagcatgggc
     1381 gtacagcact ttatcgccct tcactttctc gaaagaaggc agcaacacgt aggttttttc
     1441 ccacggtttc gggcgcggtg gctgcacggt tacggctttg gcttcctgct ttttcggtgc
     1501 caccggtttg ttatccgcgc acggcaaatc ttcaccatac ggatgcggga ttgggtcgat
     1561 ttttccaggg gtatcaagac gggtggaatc cacgccgctc cagccaggca gggcctcttt
     1621 cacgataatc gcggtattac gcacatcgcg gatttcacta atcggctcgc ccatcgccag
     1681 acggtgcgcc acctccacca gcggacgctc accgttacca aacatcagca tgtcggcttt
     1741 cgaatccacc agcacggaac ggcgcacggt atcggaccag taatcataat gtgcggtacg
     1801 acgcagacta gcctcaatac cgccgaggat caccggtaca tctttccacg cctctttaca
     1861 acgctgggta taaaccagtg tggcgcgatc cgggcgctta cccgcgacgt tatccggcgt
     1921 gtaggcatcg tcatgacgta aacggcgatc ggcggtataa cggttgatca tcgaatccat
     1981 gttgccagca gtaacaccga aaaacagatt cggtttaccc agacgcataa agtcgtcttt
     2041 gctgctccag tccggctggg cgatgatccc gacgcgaaag ccctgcgctt ccagcatacg
     2101 accgcaaatc gccatcccga agcttgggtg atcgacatac gcgtcgccag taaccaaaat
     2161 gatgtcgcag ctatcccagc caagttgatc catctcttca ctagacatcg gcaaaaacgg
     2221 tgccggtcca aaacaggcgg cccagtactg cggccaggag aacaggtcgc gatccggttg
     2281 gatcagggag atagagctca ttttgcttcc agaaatgata aaaaaataat caaaggccgg
     2341 ggattataag ccggaacgaa agagaaatcg aaaagtattc catactcgcc ctcctcgggc
     2401 gagtatgaag attacggtac cggattgact aacagttgcc caatcgagcc acggtccgcc
     2461 atttccagcg tctgactgtt gaagtagaac gggaagtgcg cccaggaagg ctgaccgaaa
     2521 taaacaagca gctccacttg tccgtcaacc caaacggtat ctttccagcc tctgtcttcc
     2581 gggaacggca tcgcaccatt cacgttacgg atctggaaca tcacgccttc aatatggaac
     2641 gcttgcggct cgtccgcgcg taccgtccag cgttcccacg ttccttgctg cgcggtgaca
     2701 tcaatacggt tgacgtccca cagttgtcca ttaatacccg ggtcatcacc cagactgata
     2761 tcgcgactgc gaatcggcga accggccatg atttcagttg gcagcaagcg catcggaaga
     2821 ctgtctgtga ccagcggcag aaggccggtt gggcgcagcg ttagcaccag ggtagaaacc
     2881 agaatgctgg atggctcaaa gaagccacga atacgatcaa caatgctcgc cgcttcgcca
     2941 caggtgatcg acacttcatc accgttgctc atatccacca gaatctcgcg gcgctcgccc
     3001 ggtgccaacg aaagttgctt caccgataca ggagcaggca ggaatccctg atcgccagaa
     3061 atcacatgta acgggcgact atcgctcatc tgtagttgat agcgacgaga gttcgacgcg
     3121 ttcagcagtc gcaagcgcac ccagccacgc gagacttcaa cgtacgggct ttgtacaccg
     3181 ttaaccagca gcgtatcacc aacaaagccg ccgcttcccg gttcgttgta ttctggcgta
     3241 ccaaagttat ccagccgttt atcctggata atgaccggaa aatcatccac accataatgg
     3301 ttggggatag gcagcgactt gctgacttca tcttccacca gccacattcc ggcaaggccg
     3361 ttatagacct gctgagccgt gcggttggga gtattggcgt gataccacag agtagctgcg
     3421 ttctggcgaa tgggcagtac gggtgcccag tcagcgtttg gcgacatcat ccgtgccgga
     3481 ccgcccatca gcgggcctgg tacctgtagc ccggccaccg tcattgagac attttctgtc
     3541 aggcggttgc tgtaaataag cttaacatcg tcgcccttcc agacgcggat agtcggcccc
     3601 aggtaacgac cattgattcc ccagaccgac gcgcgtgtcc ctggcgtaaa tgaccagtgc
     3661 gcacgttgta cagtcataaa cagcggttgc ccacggcgag attcaagtag cggcggaacg
     3721 ggtagcggtt gctgttgccc ggctgcgctg gccttcaggg gaacagcgcc ggcacaaagt
     3781 gcaatccccg atgcctgaat gaactgacgc cgactgagtg acatatttgc tccatgtaaa
     3841 actggctaaa gtaataccgt gcaggaaagt gttccccgca aaaatacaga ttgccgactt
     3901 aaacttttcc ggcggcttcg cgttctgcga cttctttatc gagctcggcg attttttgtt
     3961 ccattatcga acgacaatgg gcagccagct cacgaacctg atctttgcca tactgactga
     4021 cgtcaattgg cggcagcatt tcgacaatca ccagaccgtt gtgcagtcga ttaagattaa
     4081 tcttattcga agttgtagag acgcacacgg gaataatcgg gacgcccgcc gcaattgccg
     4141 cgtgaaatgc tccagtcttg aacggtagca ggccgcgacc acggctgcgg gttccttccg
     4201 ggaacatcca gatggaaata cggcgttttt tgaaatgatt cacgacttcc gcaatggtgc
     4261 cgtgagcttt agtgcgattg tttctgtcga tcaataagtt gccggttaac cagtacaact
     4321 gcccgaagaa ggggatccac agcaagctct ttttacctac cgtcaccgtc ggcggttgca
     4381 caatgttcga tgctgtcacc atgtcatagt tgttctggtg gttagcgata tagatagcat
     4441 tgccgtagct ttcagcatct gcaggtttgc ggcactcaac tttcaggccg aacagcggcg
     4501 caagacggcc aaacatatgc ccaaaggtgg ccacatgttt cgggttacgc gggctgaaaa
     4561 ggcagtaaat ggagccgaat acacagacta agatgctgta aatcacggta ataataagac
     4621 gaaagatata tagcataaca acctctgaag gtctgagaga ctggcattgt acgtcacctg
     4681 aaatcaggtt agggcattgt taaaagccgc tctctggaaa agcgtattgt taatcgcaac
     4741 gcatgaataa acaacggttt caagggaatt tttatcgttt ccgggggttg aatacgctgc
     4801 cggatgacga cttaacgttt catccggcgt tccttgcaag cgggaggaaa caggccctcc
     4861 ccggcatatt actcttcgct atcaccgctg ctggcacggc gaggagagtc gatctcaaca
     4921 cgatcgatac gctgcaaacc gcgcatcaac gtaccgcggc gtccacgttc gccagtgact
     4981 ttctgtaact cttccgggcg cagtttaatt ttgcgtttcc caacatgaat ggtcagcgtg
     5041 ctttgcggcg gcagaacgta caattgcgcc agaccatctt ctccacgcgc ggcttctgcc
     5101 gatggaatgt tgataatctt gttgcctttg cccttcgaca gctgcggcag atcgcttacc
     5161 gggaacatca acatacggcc tgcctgagtg attgccagca gcatatcgga agcatcttca
     5221 atcaccaccg gcggcataac atgggcattt tccggtaagg tgatcaaagc cttacctgca
     5281 cggttacgcg ccaccagatc gttaaaggtg cagacgaaac cgtaacccgc atcggaagcc
     5341 atcagcagtt tctgatcgtc gctttccatc agcatatggt caacggtcgc cccaggcggc
     5401 aacgttaatt tgccggtgag tggttcgccc tgaccacgcg ccgacggcag cgtaatcggg
     5461 tcgatggcat agctacgacc ggtggaatca acaaacacta ccggttggtt gctcttacct
     5521 ttcaccgccg ctttgaagct atcacccgct ttataattca ggcccggcgc gtcgatatca
     5581 tggcctttag cgctgcgtac ccagcccatc tgcgacagca caatggtgac aggttcagac
     5641 ggcagcatgt cgtgctcgct catcgctttc gcttcttcgc gttcctgcaa cggcgaacga
     5701 cgatcgtcac cgtaggcttg cgcgtctgcc tgcagttctt tcttcagcag gttattcatt
     5761 ttacgctcgg aaaccaaaat gccctgcaac tggtcgcgct ctttttccag ctcactctgc
     5821 tcaccgcgaa tcttcatctc ttccagtttg gcaagatgac gcagtttcag ttcgaggatc
     5881 gcttccgcct gggtttccgt aaggccaaac cgcgacatca gcgccggttt cggttcatct
     5941 tcgttacgaa tgatctcaat cacttcgtcg atgttgagaa acgccaccag caaaccttcc
     6001 aggatatgca ggcgcttgag gactttctcc agacgatagt tcagtcggcg gcgcacggta
     6061 tcgcgacgga acaccagcca ttcggagagg atttccagca ggtttttcac cgccggacga
     6121 ccatccagac cgatcatatt gaggttaatg cgatagctct tttccagatc ggtggtagcg
     6181 aagaggtggt tcatcacctg atccatatcc acgcggttgg aacgcggcac aatcaccaga
     6241 cgggtcgggt tctcgtggtc agattcatcg cgcagatcgt caaccatcgg cagctttttg
     6301 ttgcgcattt gcgcagcaat ttgctccagt acgcgcgcac ctgaaacctg atgcggcaat
     6361 gcgctgataa ccaccgcgcc atcttctttc ttccacaccg cgcgcatacg cactgaacca
     6421 cgtccgttct cgtagatttt acggatctcg gcgcgcgaag tgataatttc cgcttcagtc
     6481 ggataatccg gcccctgcac gatatccagc agctgatcga gcgtggtttt cggctggtcg
     6541 attaatgcga ttgccgcctg agctacttca cgcaggttat gcggtggaat atcggtcgcc
     6601 atgccgacgg caataccggt ggtgccgtta agcaaaatgt ttggcagacg ggcaggcagc
     6661 attttcggct cctgcaaagt gccgtcgaag tttggcaccc agtcagccgt cccctgcccc
     6721 agctcgctca atagcagctc ggaatatttc gacaaccggg attcggtgta acgcattgcc
     6781 gcgaacgatt tcggatcgtc cggcgcgccc cagttcccct gaccatcaac cagcggataa
     6841 cggtaagaga acggctgcgc catcaggacc atcgcttcat aacaggcgct atcgccgtgc
     6901 ggatggtatt tacccagtac gtcaccgacg gtacgggccg attttttaaa tttggcgctg
     6961 gcattcaggc ccagttcaga catcgcatac acaatgcggc gctgaacagg tttaagacca
     7021 tcgccaataa acggcaacgc acggtccatg atcacgtaca tggagtagtt taagtaggcg
     7081 ttttccgtaa attcatgtag cgcaaggcgc tctgccatat cgctcattaa ttctgattcc
     7141 tcaacttatt cgccagcctt caaaggggca atattgccgg agatactaca ttatctgccg
     7201 cgtcgagtca caggagatca gcgtgaatag cgccgccgga cgtgctccga cggcgataaa
     7261 ggcatcaatg tgccttgata tacaactctt tgctgtagac ttgatcggca tagagggcag
     7321 cacaaggttg cgcccatgcc acaccactcg gcatgggggt ccgctccggg cgattcgaga
     7381 agttgaggtt atgcgagtcc agggaagcct aaagcatcaa agtagtcgac tgtaagtata
     7441 cggttcagcc cagtttagct gtgtctccac cagtgccccg cgcctcctgc cataagtgcg
     7501 gcttgctcat ggataccccc gagacggctt acggcgcacc ggataataaa tttcgcgtat
     7561 acatggtatt ccttaccgga gaaattataa gcccaacaac aatacgcctg tggcattgtt
     7621 cgctcaaact tatcgcgagt gatttggctc acactccggt aaagattccc catcacgcta
     7681 acggcacata aacatccgtt tccagtcgct gttctgtcat accttcggca agattggtgt
     7741 agtgaaacag aatcggtgct ttacgcattt tctcgccgct tgcaggcagc cagtggcgaa
     7801 tgatgcccca taccgtgtgt gaaatatcgt ccagctcgcc aacgtggcgg gccacggcat
     7861 aacgtccacc ggtaagttca ccattgctca caccataacg attatcggga atcggttcgc
     7921 taacgctgcc gcagatatca aagcgaaacg ctttcggtgc ggtggtggca gggtcgtccc
     7981 aggcgacgcc aaaagtctgg ctttggttaa caggagaaag ccccgtctct ttacgccaca
     8041 tgataaattt cgccacactg tagttgagca actcagggct acagcggtgc ggcaacatcg
     8101 ccacagggat tgatgggaaa tcgataatgt ttacatccag tgtcaggttt gtcattttgc
     8161 ctccctgcga aattattccc gggaacgcgt cccgggagcg gtaacaatta cggatttact
     8221 ttgcggatct gtttgacgtc aatttcaaca gaattccagt ctttatcgac ttccccctga
     8281 atctcaaccg tatctttcgg cgtcaccgtc acaccgttcc agcgtttgtg gtcgatatca
     8341 acattgatag taccgctggc atctttaaac acgtagagat cgtcagagat gcgttcaacg
     8401 atattgccgc gcagggttac ccaggtgtcg tcacgcaggg attttgcgct ttctacagtc
     8461 gttacgctgc cgttcggccc ctggaatcct ccggcctgac tttgcgttgc cgatgggcca
     8521 gaaaaaccgc cctgctctgc tgccatcacc ggtgcactgc acagggccat tactgcgatt
     8581 actgctgcga attttttcat gtttattact ccctttaatg tctgtttccg agcatttaac
     8641 aagatagtcc ttaacaactt cttaagggaa aaaaataaaa tttaatgctg tacagagcgc
     8701 gttacaacac ggtttactgg cagcaaatac ggttatcgca gggatgaaaa aatgcgaatt
     8761 ttactgatag aagatgacat gctgattggc gacggcatca aaactggcct tagtaaaatg
     8821 ggttttagtg tcgactggtt tacacaaggt cgtcagggaa aagaggcgct ttatagcgca
     8881 ccttatgatg cggtgatcct ggatttaacc ttaccaggca tggatggtcg cgatattttg
     8941 cgcgaatggc gagagaaagg tcagcgtgag ccggtactga tcctgaccgc gcgtgatgcg
     9001 ttagcggaac gtgtagaagg gctgcgtctg ggagctgacg attatctgtg taaacctttt
     9061 gcgttgatag aagtcgccgc caggctggag gctctgatgc gccgaaccaa cggccaggcc
     9121 agcaacgagc tgcgccacgg taacgtcatg ctcgaccccg gcaaacgtat cgccacgctg
     9181 gctagcgaac ccttaacact gaaaccaaaa gaatttgccc tgctggaatt actgatgcgt
     9241 aacgctggtc gggtactgcc gcgcaaactg attgaagaga aactgtatac ctgggacgaa
     9301 gaggtcacca gtaatgccgt tgaagtgcat gtgcatcatc tgcgacgcaa actcggtagt
     9361 gattttattc gtaccgtgca tggtattggc tacacattag gtgagaaatg aaatttaccc
     9421 aacgtcttag tctgcgcgtc aggctgacgc taatcttttt aattctggcc tcggtgacct
     9481 ggctgctttc cagctttgtc gcctggaaac aaacaacgga taacgtcgat gaattgttcg
     9541 acacccaact gatgctgttt gccaagcggt taagtacgct cgatctcaac gaaatcaacg
     9601 cggcggatcg catggcacag acgccaaata aattaaaaca cggtcatgtt gatgacgatg
     9661 cgctgacctt tgccatcttt acccacgacg gcagaatggt ccttaatgat agcgataacg
     9721 gagaagatat tccctatagc tatcaacggg aaggttttgc tgacgggcaa ctggtcggtg
     9781 aagacgatcc ttggcgtttt gtctggatga cctcacctga tggcaaatat cgcatcgttg
     9841 ttggccagga atgggaatac cgtgaagaca tggcgctggc gattgttgcc gggcaattga
     9901 tcccgtggct ggtcgcactg ccgattatgt taatcatcat gatggtacta ctgggtcgtg
     9961 aactcgcgcc gctgaacaaa ctggcgctgg cactacgtat gcgtgaccct gactcggaaa
    10021 aaccactaaa cgcgactggc gtacccagcg aagtgcgacc actggttgag tcgctaaatc
    10081 aactgttcgc ccgcacacat gcgatgatgg ttcgagaacg acgctttacc tccgatgcag
    10141 ctcacgaact acgtagcccg ttaacagcgc tgaaagtgca aaccgaagtt gcgcagctct
    10201 ctgacgatga tccgcaggca cggaaaaaag cactgctcca attacattcc gggatcgatc
    10261 gcgctactcg tctggttgat caactgctca cgctatcgcg gctggactca ctggataacc
    10321 ttcaggacgt cgcggagatc ccgcttgaag atctcctgca atcgtcggtg atggatattt
    10381 accacacggc gcagcaggcg aaaattgacg tgcgactgac actcaatgtc cagggcatca
    10441 aacgcaccgg acaaccgcta ttgctaagtt tgttggtgcg aaatttgctg gataacgccg
    10501 tgcgttacag tccacagggc agcgtggtgg acgtcacgct gaatgccgat aatttcatcg
    10561 tgagggataa cggccccggt gtgacaccag aggcactggc gcgaattggc gaacgcttct
    10621 atcgcccctc cggacaaacc gctaccggca gtggacttgg gctatcgatt gtccagcgaa
    10681 tcgccaagct gcatggcatg aatgttgagt ttgggaatgc ggaacaaggt ggatttgagg
    10741 cgaaagtaag ctggtaaggg taagactttt gctaaattcg caaaagactt tgcacatttt
    10801 gctaatttca ccgtaccgct ctgtgacgta ctatagtcgg caaacgtctc accttgaggt
    10861 taaaaaatga gcaacattct gattatcaac ggcgcgaaaa aattcgccca ctccaatggt
    10921 caactgaatg acaccctgac cgaagtcgcg gatggcacac tgcgcgacct cgggcatgat
    10981 gttcgcatcg ttcgcgccga cagcgactac gatgtcaaag cggaagtaca aaactttctc
    11041 tgggctgatg tggtgatctg gcagatgcca ggctggtgga tgggcgcgcc gtggacagtg
    11101 aaaaaataca ttgatgatgt attcaccgaa ggtcacggga cgctgtatgc cagcgatggc
    11161 cgtacccgca aagatccgtc aaaaaaatac ggttccggcg gcctggtaca gggcaaaaaa
    11221 tatatgcttt ctctgacctg gaacgcacca atggaagcct tcaccgaaaa agatcaattc
    11281 ttccacggcg ttggcgtcga cggtgtgtat ctgccgttcc ataaagcaaa ccaattcctt
    11341 ggcatggaac cgctgccgac atttatcgct aatgacgtga taaaaatgcc tgatgttccc
    11401 cgctatactg aagaatatcg caagcatctt gtggaaattt ttggttaact agagctcagg
    11461 ctttagaagg agttaaccat gcttaccgta atcgcagaaa tccgtactcg tcctggtcaa
    11521 catcaccgtc aggcggtatt ggatcagttt gctaaaatcg ttccaaccgt actgaaagaa
    11581 gaaggttgcc acggctatgc gccaatggtg gattgcgctg ctggcgtgag tttccagtct
    11641 atggcaccgg attctatcgt gatgattgag cagtgggaaa gcatcgcgca ccttgaagcg
    11701 catctgcaaa ccccgcacat gaaggcgtac agcgaagccg taaaaggtga cgtgctggag
    11761 atgaatattc gtattctgca gccagggatt taatcctgcc ttgtttgccc ggccatcctg
    11821 accgggcaat gttctttcct ttaaacctca atctccgcca tgtcgccttt ctcttgcaac
    11881 cagttgcggc gatcttccga gcgtttcttc gccagtagca tgtccatcat cgcgtcagta
    11941 cgctgatcgt cttcatcatc gatagtcaac tgcaccagac ggcgagtgtt cggatcaagc
    12001 gtggtttcgc gcaattgcat cgggttcatt tcccccagac ctttaaaacg ctggacgttc
    12061 ggcttgcctt tcttgcgttt taattgctca agtacgccct ctttctcttc ttccgtcagc
    12121 gcgtaataaa cctctttccc gagatcaata cggtagagcg gtggcagtgc gacgtaaacg
    12181 tgaccgtgtt tcaccaacgc gcggaagtgt tttacaaaca aagcgcagag cagcgtggca
    12241 atgtgcagac catcggagtc agcatccgcc aggatacaga tcttgccgta acgaagctgg
    12301 ctcaaatcgt cgctgtcagg atcgataccg atcgctaccg aaatatcgtg cacttcctgc
    12361 gaagccagca cttcgtcgga agagacttcc caggtgttaa ggatcttacc tttcagtggc
    12421 atgatcgcct gatattcgcg atcgcgcgcc tgcttggcag atccgcctgc ggagtcacct
    12481 tccacaagaa acagttcggt gcggttaagg tcctgcgcgg tacagtcagc cagtttgcca
    12541 ggcagcgccg gaccgctggt cagcttttta cgcaccactt ttttagccgc acgcatacga
    12601 cgctgggcgc tggaaatcgc catctctgcc agcaactccg ccgcctgaac gttctggttc
    12661 agccacagga taaaggcatc tttcaccacg ccagaaacga atgccgcgca ctgacgcgaa
    12721 gagagacgct ctttggtctg cccggcaaac tgcggatcct gcatttttac tgacagcaca
    12781 taggcgcagc gatcccagat atcttccgcc gacagcttta caccgcgcgg cagaatgttg
    12841 cggtattcac agaactcacg catcgcgtcc agcaggccct gacgcagacc attaacatgg
    12901 gtaccgccct gcatcgttgg gatcaggttg acgtagcttt cggtcagcag ttcaccgcct
    12961 tccggcagcc acagtagcgc ccagtccacc gcttcagtat cgccagcgaa attaccgata
    13021 aacggttttt ctggcagcgt cggtaaaccg tttaccgctt ccgccaggta atcattcaga
    13081 ccgtcctgat agcaccagcg ctgttcggtg ttgttgatct catctttaaa ggtaatctca
    13141 acgccggggc acagtactgc tttggctttc agcacatgcg tcaggcgtga aacagaaaaa
    13201 cgcgggctat cgaagaaggt ttcatccggc cagaaatgga cgctggttcc ggtattgcgt
    13261 ttaccgcaag tgccgacaac ctgtaaatcc tgcacctttt cgccattttc aaaggcgatg
    13321 ttataaatct gaccatcgcg gcgcacgtta acttctacgc gcttcgacag ggcgttaacc
    13381 accgaaatcc ccacgccatg caggccgcca gagaactggt aatttttgtt agagaattta
    13441 ccgcctgcat gcagacggca aagaatcagt tcaaccgccg gtacaccctc ttccgggtga
    13501 atatccaccg gcatcccgcg cccatcgtca ataacttcta acgactggtc agcatgtaaa
    13561 ataacgtcca cgcgttttgc gtgacccgcc agtgcttcat ccacactgtt atcaatgact
    13621 tcttgcccca aatggttagg gcgagtggta tcggtataca tccccggacg gcggcgaacc
    13681 ggctcaagcc cggtgagtac ctcaatggca tcagcgttat aagtttgcgt catggtttaa
    13741 gttagtaatt cgagttgatc gtcagagatg gtgcagacca agaaaatcga cgatcgggtt
    13801 gaaataatct tcgaagcccg tgaatgcgtg gttgccgcct tctatgacag tctggcggca
    13861 ggaagcgtag tacgccaccg cctggcggta atccagcact tcatctcccg tctgttgcag
    13921 cagccagatc aaatccggcg cttccagcgg gtcaatctgc atgactttaa gatcgtaaat
    13981 atggcgtgac tctagcacat attgctgccc ggtgtagggg ttctcgttct gaccgagata
    14041 gtccgtcagc agttcaaacg ggcgcaccgc cgggtttacc accactgcgg gcagcataaa
    14101 acattgtgac aaccaggtgg cgtaatatcc ccccagtgac gaaccgacaa tacccagcga
    14161 atcgccgcca tgttccagga caatggattc cagcagctct gccgcgtcgg aaggatacgg
    14221 cggcaactgc ggaatgatca tctcaacgtc agggtgatgt tccgccagcc agttttttaa
    14281 caagctcgct tttgcagagc gcggcgagct gttgaaaccg tgtaaataaa gaagcgtaga
    14341 catcagtagc cttctgaagc ggtatcaggt tggaaacgtg tgtccgccag gcgatgcacc
    14401 tcggtggtca gcgtgccatc agcatgtaac tcgagagtac gccagccggg cgcgatggta
    14461 tccagcgtaa agttggaaca gtgcggctta aactgcacac aggtcgacgg cgttgccagc
    14521 aggcggcgac cgttccagtc gagatccagc tcctgatgaa tatgaccgca cagcaagtat
    14581 ttgacgtgcg gaaacttcgc cagcacggta tccagttcgc ccgcgttacg cagactgtgt
    14641 tgatcgagcc aactacaacc cgcaggtagc ggatgatgat gcagcagcag caacgtatgg
    14701 cgttctggcg catcggccag tttacgttcc agccactcaa gctgaaactc gctcagctca
    14761 ccgtgcggca cgccaaacac ctggctatcc agcaacagga tttgccattg ctcaccaata
    14821 aacacgcgct tcgccgggga gatacccgca tcctgtaacg cgctgtacat cgcgggctgg
    14881 aaatcgtggt tgcccggcag ccagacgcag ggcgcacgaa aacttgcgat gccttcagcg
    14941 aaatgctgat aggccgcaga ggattgatcc tgcgctaaat cacctgtcgc gacaatcagg
    15001 tcgaattcgt gctgatgtgg acgaatcgcc tccagcaccg cctggtagct ttcccaggtg
    15061 tttaccccta acagggcttc gtgcttttgt gcaaacaggt gagtatcggt aatttgtaaa
    15121 atcctgactc tggcctcacc agccagagga agggttaaca ggctttccaa atggtgtcct
    15181 taggtttcac gacgctagta aaccggaatc gccatcgctc catgtgctaa acagtatcgc
    15241 aaccagtccg ctaaaaactg gttaatttga tgcttttcgt cgcgttgatg caacttttta
    15301 ttaggataat cataccgcgc tttgaagcga aaaatctgct ggcttgaaca cacttcagcc
    15361 accatcgcgt catgatacag acgcaccgtc attgacggaa ggctccagta actgatcgcg
    15421 ggcgcagtct gttctattgt caccagggta gtgtatcggg tcgattccac agtcgtcagc
    15481 cgatattgtg cgtttgccac ctgatagctt acagtttcgc cgggtgcgtc attgcgcggt
    15541 aacaaacggc gcaattgtga aaagttcatc tcgcacaggc gcatcatttc aggaaagtca
    15601 ggtgtgtaac gcttcattta tgcccactca ttttttaacg cttggtggtg cagctgcagc
    15661 cattgcaaag cgatgaccga cgctgcgttg tcgattttcc cctcttctac ccactggtat
    15721 gcctgttccc ggcttaccac atgaacgcga atatcttcgt tttcatcagc cagaccatga
    15781 ataccgcttg cggtcgtggc gtccacttcg cccaccataa ttgacgaacg ctcactggtg
    15841 ccccccgggc ttgccaggaa acttaacacc ggtttggtcc gtttgactat cagccccgcc
    15901 tcttcaatcg cttcgcgacg ggcaacatct tccacacttt caccctcttc aatcatcccg
    15961 gcaaccatct ccagtagcca gggggtttcg ctagtgtcgt acgcggcaat tcgaatctgc
    16021 tcaatcagca caacttcatc acgcactggg tcaaagggta gcaagactgc ggcgtgaccg
    16081 cgctcaaaaa tttcccgccg cacctcatga ctcatttgcc cgttgaatag actatgacga
    16141 aatctataaa gatctaatga aaaaaagccg cgataaagtg tttctcgtgc aataatttct
    16201 acatcgtttt tgccaaatgt aacgggcagg ttgtctggct taagcattgt taatgtcctg
    16261 gcactaatag tgaattaaat gtgaatttca gcgacgtttg actgccgttt gagcagtcat
    16321 gtgttaaatt gaggcacatt aacgccctat ggcacgtaac gccaaccttt tgcggtagcg
    16381 gcttctgcta gaatccgcaa taattttaca gtttgatcgc gctaaatact gcttcaccac
    16441 aaggaatgca aatgaagaaa ttgctcccca ttcttatcgg cctgagcctt tctgggttca
    16501 gttcgttgag ccaggccgag aacctgatgc aagtttatca gcaagcacgc cttagtaacc
    16561 cggaattgcg taagtctgcc gccgatcgtg atgctgcctt tgaaaaaatt aatgaagcgc
    16621 gcagtccatt actgccacag ctaggtttag gtgcagatta cacctatagc aacggctacc
    16681 gcgacgcgaa cggcatcaac tcgaacgcga ccagtgcgtc cctgcagtta actcaatcca
    16741 tttttgatat gtcgaaatgg cgtgcgttaa cgctgcagga aaaagcagca gggattcagg
    16801 acgtcacata tcagaccgat cagcaaacct tgatcctcaa caccgcgacc gcttatttca
    16861 acgtgttgaa tgctattgac gttctttcct atacacaggc acaaaaagaa gcgatctacc
    16921 gtcaattaga tcaaaccacc caacgtttta acgtgggcct ggtagcgatc accgacgtgc
    16981 agaacgcccg cgcgcagtac gataccgtgc tggcgaacga agtgaccgca cgtaataacc
    17041 ttgataacgc ggtagagcag ctgcgccaga tcaccggtaa ctactatccg gaactggcgg
    17101 cgctgaatgt cgaaaacttt aaaaccgaca aaccacagcc ggttaacgcg ctgctgaaag
    17161 aagccgaaaa acgcaacctg tcgctgttac aggcacgctt gagccaggac ctggcgcgcg
    17221 agcaaattcg ccaggcgcag gatggtcact taccgacgct ggatttaacg gcttctaccg
    17281 ggatttctga cacctcttat agcggttcga aaactcgtgg tgccgctggt acccagtatg
    17341 acgacagcaa tatgggccag aacaaagtgg gcctgagctt ctcgctgccg atttatcagg
    17401 gcggaatggt taactcgcag gtgaaacagg cccagtacaa ctttgttggt gccagcgagc
    17461 aactggaaag cgcgcatcgt agcatcgtgc aaaccgtacg ttcctccttc aacaacatta
    17521 atgcatctat cagtagcatt aacgcctaca aacaagccgt agtttccgct caaagctcat
    17581 tagacgcgat ggaagcgggc tactcggtcg gtacgcgtac cattgttgat gtgttggatg
    17641 caaccaccac gctgtacaac gctaagcaag agctggcaaa tgcgcgttat aactacctga
    17701 ttaatcagct gaatattaag tcagccctgg gtacgttgaa cgagcaggat ctgctggcac
    17761 tgaacaatgc gctgagcaaa ccggtttcca ctaatccgga aaacgttgcc ccgcaaacgc
    17821 cggaacagaa tgctattgct gatggttatg cgcctgatag cccggcaccc gtcgttcagc
    17881 aaacatccgc acgcactacc accagtaacg gtcataaccc tttccgtaac tgatgacgac
    17941 gacggggctt cggccccgtc tgaacgtaag acaacgtaaa gatacgggtt atctgccgca
    18001 ttcttccccc ttctcgcttc aatttcgacc agccatcctc tattctgatg ggtatttacc
    18061 actggtcccg gaagacaaaa atgaaacgga caaaatccat acgccacgca tcgttccgca
    18121 aaaactggag cgcacgccat ctgacaccag tcgctctcgc ggttgccact gtttttatgc
    18181 tggctagctg tgaaaagagt gatgaaacag tgtctctcta tcaaaatgct gacgactgtt
    18241 cagctgcaaa cccaggcaaa agcgccgaat gtaccaccgc gtacaacaat gcgctgaaag
    18301 aagccgaacg tactgcgccg aaatacgcca cccgtgaaga ctgtgttgct gaatttggtg
    18361 aaggtcagtg ccagcaggca ccagcccagg ctggcatggc accagaaaac caggcgcagg
    18421 cccagcaatc cagcgggagt ttctggatgc cgctgatggc cggttacatg atggggcgtc
    18481 tgatgggcgg cggcgcggga tttgcacagc agccgctgtt ctcctcgaaa aacccagcca
    18541 gcccggctta cggtaaatat accgacgcga cgggtaaaaa ctatggcgca gcccagccag
    18601 gccgcaccat gaccgtaccg aagacagcca tggcaccaaa accggcgacc actactaccg
    18661 ttacccgtgg cggttttggt gaatctgttg ccaaacaaag cactatgcag cgtagcgcaa
    18721 ccggcacctc ttctcgttca atgggtggct gataccgatg gaaagagtca gtattaccga
    18781 gcgcccggac tggcgtgaaa aagcccacga atacggtttc aattttcaca ccatgtacgg
    18841 cgagccgtac tggtgtgaag atgcttacta caagttgccc ctcgcccagg ttgaaaagct
    18901 ggaagaagtc accgccgaac tgcaccagat gtgcctgaaa gtggtggaaa aagtgatcgc
    18961 cagcgatgag ctgatgacca aattccgcat tccaaaacac acctggagtt ttgtgcgtca
    19021 gtcatggctg actcaccagc catcgcttta ttcgcgtctt gatctggcat gggatggcac
    19081 tggcgaacct aaactgctgg aaaataacgc cgatacacca acgtcactat acgaggcggc
    19141 gttctttcag tggatctggc tggaagatca gcttaacgcc ggtaacttgc cggagggcag
    19201 cgaccagttt aacagtctgc aagaaaagct aatcgatcgc tttgtcgagt tgcgtgaaca
    19261 gtatggcttc cagttgctgc atctcacctg ctgtcgcgac acggtggaag atcgcggaac
    19321 cattcagtat ttgcaggact gcgcgacgga agctgaaatc gctaccgagt tcctctacat
    19381 tgacgatatc gggttaggtg aaaaaggtca gttcacggat ttacaggatc aggtaatttc
    19441 caacctgttc aagctgtatc catgggaatt tatgttgcgt gagatgttct ccaccaagct
    19501 ggaggatgca ggcgtacgct ggctggaacc ggcgtggaag agcattatct ccaacaaagc
    19561 gcttctaccg ctactgtggg agatgttccc gaatcacccg aacctgctgc ccgcttattt
    19621 tgcggaagat gatcatccgc aaatggaaaa atatgtggtt aaaccgatct tctcccgtga
    19681 aggcgcaaac gtgtcgatca ttgagaacgg caaaaccatt gaagcagcgg aaggtccgta
    19741 tggcgaagaa gggatgattg ttcagcaatt ccatccgtta ccgaaattcg gcgacagcta
    19801 tatgctgatt ggtagctggc tggtgaacga tcaacccgcc ggaattggca ttcgtgaaga
    19861 ccgtgcattg atcacccagg atatgtctcg gttttatcca catatttttg ttgagtaatc
    19921 aatgatgccg gatggcattc gccatccggt aattgttaac ctatctgcac cgacagcata
    19981 ctcaggctgc ccatttctat accatcgacc ggaatggtaa ttggctcctg cccatcccac
    20041 gcacctaaga catacaacaa cggcagatag tgctccggcg ttgggttcga taacgcgcca
    20101 ccttcatggt cgaggtaatt caccagagga tgctgctcta ccgccccttg ccacgtcaga
    20161 ttcgctttca catactcatt aaacgacatc gcccaaggat atggtgaact atcaccgtgc
    20221 cacttcactg tacgcaggtt atgcaccacg ttgccactgg cgaccaacat tattccttca
    20281 tctcgcagcg ctgccagttt gcgccccatt tcgaaatgcc aggcggcagg tttgctactg
    20341 tcgatactca actgcaccat cgggatatcg gcgtcaggat acatcttaat cagcacgccc
    20401 caggaaccgt ggtcaaagcc cctggcttct ttatccagcg tcaccgggac cggcgctaac
    20461 agctcaacca gatgctgtgc cagcacaggc gaacccggag caggataatg cgtatcgtac
    20521 agcgcctgcg ggaagccacc aaagtcatga atcgtgggcg gcgtctccat cgcggtcact
    20581 cctgttccac gggtaaacca gtgagccgaa accaccacaa tcgcttgcgg gcgtggcaat
    20641 gtcatcccca acttctgcca gctgcgggta tacaaattat cttccagcac gttcatcgga
    20701 ctaccgtgac ctaaaaacaa tgctggcata cgtgttgaag acatgatgat atccttaact
    20761 aagggtgtca ttttgatatc ctcacaatac acttgttcgg cggagtaaga acccggataa
    20821 caatgatgat gatcatcagt tattttgacg atctgcctga aggtgaagat ttataaggag
    20881 ttgtcgatgt cagtacctct cattctgacc atactggcgg gggcagccac gtttattggc
    20941 gcgtttctcg gcgttctcgg acaaaaaccc tcgaaccgct tactggcgtt ttcgctaggt
    21001 tttgcggcgg ggatcatgtt gctcatctca ttaatggaaa tgcttcctgc cgcactagca
    21061 gctgaaggaa tatcgcctgt gttgggttat ggaatgttta tcttcggtct gcttggctac
    21121 tttggcctgg accgcatgtt gccgcatgcg catccgcagg atttaatgca aaaatcggtg
    21181 cagccgttgc caaaatcgat caagcgcaca gccattctgc tcactctcgg catcagtctg
    21241 cataacttcc cggaagggat tgccaccttt gtcacggcga gcagcaacct ggagctggga
    21301 tttggcatcg cactggccgt cgcgttgcac aatatccctg aaggtctggc agtggcaggc
    21361 ccggtttatg cggcaacggg gtctaaacgc accgcgattc tgtgggcggg gatttctgga
    21421 ctagcagaaa ttcttggtgg tgtgctggcg tggttaatcc tcggtagcat gatttccccg
    21481 gtggtcatgg cggcggttgc aggaattatg gtggcgctct cggttgatga attaatgccg
    21541 ctcgccaaag agattgaccc taataataac cccagctatg gcgtactatg tggaatgtca
    21601 gtgatgggat tcagtttagt gctgctacaa acggcgggaa ttggttaaaa acaacatcag
    21661 aagttgtcca gcacatggac aacttctgcc agcttcacga aagccttatt ctaccttttc
    21721 ggctgtctct tctctcgtac tgtttaagta tttgttccgc gcttcaagca gtgcgtctct
    21781 gtcctggcga aacgcccgac aataacgttt caaatgcagg aaataaccca ccacaaccag
    21841 agcaatcaat acaatccagt accaggcgat aaacatcctt tacccttaat atttaacaat
    21901 actaaacatt tagcgcataa atttcacata tcctttttcg gatatattca tatggtcgca
    21961 taataacgga acaattatat gattttgatc ataatgaaaa agaatgcctg atattaatga
    22021 aattttctca tagaaagaga agcggaatta acatcttgca tagcagggca aaataaaaac
    22081 cgaagccagc tacaacttcg gtttcttgat taaggcagta attaagcagc ggttttcagc
    22141 tggctttacg ctcatgtgcc tgacggtatg ccaccaggtc ttcaatagtc acgagcgcca
    22201 tattgtgttt attggcaaac tcaatgcact ctggtgcacg cgccatcgtg ccatcgtcat
    22261 tagtcagctc acacagcaca ccagccggtt taaagcctgc cagcgtcatc aaatcaatag
    22321 tcgcttcagt atgaccgcca cgcgtcagta caccacctgc ctgcgcgcga agtgggaaaa
    22381 cgtggcccgg gcgattcaga tcagaaggtt ttgcgccatc ggcaatcgct gcgcgaacgg
    22441 tcgtaatacg atcagcggca gaaacaccgg tagtcacacc ttcagccgct tcaatggtca
    22501 cggtaaaacc ggtgccatag gcgctggtgt tattttctac catcattggc agatcaagtt
    22561 gtttacggcg atcttcagtg atgcacaggc aaacaatacc gctaccgtgg cgaatggtca
    22621 gcgccatctg ctcaacggtc atggtttctg ccgggaagat catatcgcct tcgttttcac
    22681 ggtcttcatc atcaagcacc attacaccgc gtccttcacg cagcgcagcc agtgcatttt
    22741 caacacgttc gaaaggcgta ccaaaagagg aaagtagcgt ctgattcatg gtaaaaaaaa
    22801 ctcactaaaa ttatggttac cagaatcagg gcagtcttag gagtggcggc atatagccaa
    22861 aataacgtga gcgggtccat gcccgacaga atcgttactc tctcccatcc ggactctaac
    22921 cgtcggcccc ggaattacac cggatctgct gtcctttgag ttcgcaccca aagcgctcgc
    22981 gggctttcaa ctgagttgat ttaccgccgg tggggaattt cgccccgccc tgagaataag
    23041 cggattcact ataacgctaa tgattagcgg cagcaacgca tagcttcaca taattctggt
    23101 ttatgactta cccttatcgc actacaatgg tactcaacac ctatcagtac agggaaacta
    23161 caatgattga cccgaaaaaa attgagcaaa tcgctcgcca agttcacgaa tcaatgccta
    23221 aaggaatcag ggagttcggg gaagatgtgg agaaaaaaat ccgccaaacc ctacaagctc
    23281 agctgacgcg cctcgacctt gtaagccgtg aagagttcga cgtccaaacg caagtcctgt
    23341 tacggactcg tgagaaactg gcgctgcttg aacaaagaat gagcgaactg gaaaatcgca
    23401 gtactgagat taaaaagcag ccagatccag agactctccc cccaacgctg taaacatttg
    23461 tctttaatgc actgtaaatt atgaattaat cttctaattt acagtgcttt ctattacctt
    23521 atcgcttaat tccattcaga atatcaccac aaaaatgaat gagtgcaatt gaaaatatta
    23581 ttgtcatgta tttttttaag ttttacttaa tccatattta gataaactaa tttcataaaa
    23641 cccgaaaagt aaaatataat atttgaatga ttagcaatcc ttaaataaca gttactaatc
    23701 attcattttc tacttatatt tatataaagg aatattatgt ctgcttttaa aaagtcactt
    23761 cttgttgctg gcgtagcaat gattctttcc aataacgtat ttgctgacga aggtcacggc
    23821 attgttaaat ttaaaggtga agttatctct gctccatgtt caattaaacc aggtgacgaa
    23881 gacttaactg taaatctggg tgaagtagca gataccgttc tgaaaagcga ccagaaatct
    23941 ttggctgagc ctttcactat tcacctgcaa gactgcatgt tgagccaggg tggaaccact
    24001 tattcaaaag cgaaggttac tttcaccaca gcgaatacta tgacgggtca gactgacctg
    24061 ctgaaaaaca ccaaagaaac tgaaatcggt ggtgccacag gcgttggcgt acgtattctg
    24121 aatagccaga gcggcgaagt gactttaggt actccggtag taattacatt caacaatact
    24181 aacagctatc aggaactgaa cttcaaagtt cgtatggaat ccccgggcaa agatgctacc
    24241 ccgggcaacg tttacgctca ggctgactat aagattgctt acgagtaatc acattaggcc
    24301 gggttcgccc ggccacatca ttaacggatt aatgataagt ggaacagatg tataaaaaat
    24361 taaaattaac cacaataagc gaattgatta aaaatattta ttgttcatta tccgttctca
    24421 tcattggttg tgcgtcagct tatgccgttg aattcaacaa agatttaatc gaagccgaag
    24481 atcgtgaaaa cgttaacctt tcccaatttg aaactgatgg ccaattaccc gtaggtaaat
    24541 actcattaaa cgctattatt aataataaaa ggacgccaat ccatctggac ctgcaatggg
    24601 tattaattga taaccaaact gcagtttgtc tgactccaga gcaattaaca ttattaggct
    24661 ttactgatga gattattgaa gaggctcagc aaaacctgat cgatggttgt taccctatcg
    24721 aaaaagaaaa acaaattaca acttatctcg ataaagggaa aatgcaatta tccatatctg
    24781 cacctcaggc atggttaaaa tacaaagatg caaactggac gcctcctgaa ctttgggatc
    24841 atggtattgc tggggcattt cttgactaca atttatatgc ctctcattat gcaccacatc
    24901 agggcgataa ttcacaaaat ataagttcct atgggcaggc tggggttaat cttggggcct
    24961 ggcgcctgcg tactgattac cagtacgatc agtcatttaa caatggcaaa agccaggcga
    25021 acaacctgga ttttccgcgt atttatttgt ttcgcccaat cccagcaatt aatgcaaaac
    25081 taactatagg tcaatacgat actgaatcct ctattttcga ctctttccat ttttctggcg
    25141 tttcgttgaa aagcgatgag aatatgttgc cgcctgatct acgtggttac gcaccgcaaa
    25201 tcacgggtgt tgcacaaacg aatgcaaagg tcactgtctc acagaacaac cgtattattt
    25261 atcaagaaaa tgttcctcct ggcccatttg ctattactaa tttattcaat acattacagg
    25321 ggcaactgga cgtcaaggtt gaagaagagg acgggcaagt cacgcaatgg caagttgcat
    25381 ctaatagtat tccttatctg acgcgtaaag ggcagattcg ctacaccact gctatgggta
    25441 aaccgaccag cgttggtggt gattccttac aacaaccctt cttctggact ggtgaattct
    25501 catggggttg gctgaacaat gtatccctgc atggtggttc agttttaaca aaccgtgatt
    25561 atcaatctct ggctgccggc gttggcttta accttaactc attgggttca ttatcttttg
    25621 atgtcacacg atctgatact cagttgcata atcaggataa agaaacgggt tatagctacc
    25681 gcgctaacta ttcaaaacgt tttgaatcta ccggtagcca gctcactttc gctggttacc
    25741 gtttctctga taaaaacttt gtgacaatga atgaatatat caatgacact aaccattaca
    25801 cgaat
//
LOCUS       DADLFA010000039        25633 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000039 DADLFA010000000
VERSION     DADLFA010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25633
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.039"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..423)
                     /locus_tag="JQH65_001387"
     CDS             complement(<1..423)
                     /locus_tag="JQH65_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="required for cellulose biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HAY6138856.1"
                     /translation="MRDIVGPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDYQGPEKIKLFSMLNHEKGLYYLTRDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLT"
     gene            696..884
                     /locus_tag="JQH65_001388"
     CDS             696..884
                     /locus_tag="JQH65_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6138857.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            896..1648
                     /gene="bcsQ"
                     /locus_tag="JQH65_001389"
     CDS             896..1648
                     /gene="bcsQ"
                     /locus_tag="JQH65_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HAY6138858.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHRQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAIAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            1645..4257
                     /gene="bcsA"
                     /locus_tag="JQH65_001390"
     CDS             1645..4257
                     /gene="bcsA"
                     /locus_tag="JQH65_001390"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HAY6138859.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPLDPVRYLIQTCWLLIGTSRKETPKPRRR
                     AFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSLIL
                     ALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRYTS
                     TLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVDIF
                     VPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIARTTH
                     EHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHFFS
                     PDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIAVE
                     TVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLDNP
                     LTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFVLP
                     HMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGGLV
                     EEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVLGG
                     AVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQILEG
                     QKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWALWQ
                     DSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFILRRPER
                     SETAQPSDQALAQQ"
     gene            4268..6607
                     /gene="bcsB"
                     /locus_tag="JQH65_001391"
     CDS             4268..6607
                     /gene="bcsB"
                     /locus_tag="JQH65_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HAY6138860.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYMPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETRSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERVWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            6614..7720
                     /gene="bcsZ"
                     /locus_tag="JQH65_001392"
     CDS             6614..7720
                     /gene="bcsZ"
                     /locus_tag="JQH65_001392"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HAY6138861.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            7702..11175
                     /gene="bcsC"
                     /locus_tag="JQH65_001393"
                     /pseudo
     CDS             7702..11175
                     /gene="bcsC"
                     /locus_tag="JQH65_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            11257..13245
                     /gene="hmsP"
                     /locus_tag="JQH65_001394"
     CDS             11257..13245
                     /gene="hmsP"
                     /locus_tag="JQH65_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HAY6138862.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            13428..14714
                     /locus_tag="JQH65_001395"
     CDS             13428..14714
                     /locus_tag="JQH65_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HAY6138863.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            14934..16430
                     /locus_tag="JQH65_001396"
     CDS             14934..16430
                     /locus_tag="JQH65_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAY6138864.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(16526..17674)
                     /locus_tag="JQH65_001397"
     CDS             complement(16526..17674)
                     /locus_tag="JQH65_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAY6138865.1"
                     /translation="MFKSDAARTRQRLSARKGRTLPPGRGKFPHSTTESFRNTFWLKK
                     IMEHCFNIVDQQTTTAQTNANFLQIRFTTMSKKIAVIGECMIELSEKGADVKRGFGGD
                     TLNTSVYIARQVDPAALTVHYVTALGTDSFSQQMLDAWHGENVDISLTQRMENRLPGL
                     YYIETDSTGERTFYYWRNEAAAKFWLESEQSTAICEELANFDYLYLSGISLAILSPTS
                     REKLLSLLRECRANGGKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDA
                     LWGQQPVEDVIARTHNAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTA
                     AGDSFSAGYLAVRLTGGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            17687..18454
                     /gene="pdeH"
                     /locus_tag="JQH65_001398"
     CDS             17687..18454
                     /gene="pdeH"
                     /locus_tag="JQH65_001398"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HAY6138866.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLGDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            18524..20584
                     /locus_tag="JQH65_001399"
     CDS             18524..20584
                     /locus_tag="JQH65_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HAY6138867.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(20818..22140)
                     /locus_tag="JQH65_001400"
     CDS             complement(20818..22140)
                     /locus_tag="JQH65_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HAY6138868.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLLILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETQHQSL"
     gene            complement(22551..23564)
                     /gene="yhjD"
                     /locus_tag="JQH65_001401"
     CDS             complement(22551..23564)
                     /gene="yhjD"
                     /locus_tag="JQH65_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HAY6138869.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(23613..24584)
                     /locus_tag="JQH65_001402"
     CDS             complement(23613..24584)
                     /locus_tag="JQH65_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY6138870.1"
                     /translation="MLEKTINNAICALLFRCEQQSVKEMDKIHAMQLFIKVAELESFS
                     RAADFFALPKGSVSRQIQALEHQLGTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLNE
                     LDGLFQQDATSISGKLRIDIPPGIAKNLLLPRLSEFLYLHPGIELELSSHDRPVDILH
                     DGFDCVIRTGALPEDGVIARPLGKLTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTP
                     HLGVHPLGFEVASVNGVQWFKSGGMLTVNSSENYLAAGLAGLGIIQIPRIAVREALRA
                     GRLIEVLPGYRAEPLSLSLVYPQRRELSRRVNLFMQWLAGVMKEHLD"
     gene            25032..>25633
                     /locus_tag="JQH65_001403"
     CDS             25032..>25633
                     /locus_tag="JQH65_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY6138871.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         6035 a         6481 c         7124 g         5993 t
ORIGIN      
        1 ggttaacctg ctccatttat tcattttatc caaccatgag cgaagccgct cggcaggaat
       61 gttttgccat gcgttatttg cgcaaacaag aataaaaagg taattatggg gatcaataga
      121 acactgcaaa tcacgggtca aatagtatag acctttttca tgatttagca ttgaaaataa
      181 ttttattttt tccggccctt gataatcatc taattgaaag attttcgccg gatcgctgtc
      241 catgctaatg accgcgacgt gtgcggtttc agcctgggat gcaattgttt gattcgccag
      301 actgatagca tcttcatggc gatcgacgtt aaaccaccag acgccgcctg ctggcatatg
      361 tcgcagctca tcccataatg atgagatacc gatagagaat acagggccca caatgtccct
      421 catgtcacca ttttgtctga ttatcagttt actagcgaaa gctaagaaat aaacctacca
      481 ttgaaattaa aacttatcag atttagcatg tgaatcaatt tagaaggtca caagacgtgt
      541 gattttagtc ttttcgtttt taactttttc tgttgcgtaa cggaaagtca aaaagtgagc
      601 aaattcccgt ctcgccgtaa acaataaacc ggcgaaatgc tcacggttag aattgtctca
      661 tgaacggtac ggttatttca tagggatcaa gcaaaatgaa taacaatgaa ccagatactc
      721 tgcctgatcc cgcgataggc tatatcttcc agaatgatat tgtggcgtta aagcaggcat
      781 tttcactgcc tgatattgat tatgccgata tttcccaacg cgaacagttg gccgcggcat
      841 taaaacgctg gccgttgctg gcagagtttg cgcaacaaaa gtaggggatt ggtgaatggc
      901 cgtactggga ttgcaggggg tgcggggagg cgtggggaca acaaccatca ccgccgcatt
      961 agcctggtca ttacaaatgt tgggagaaaa tgtcctggtg gtcgatgcct gcccggacaa
     1021 cttgttgcgc ctgtcattta atgttgattt tacccaccgt cagggctggg ccagagcgat
     1081 gctggatggc caggactggc gtgacgctgg gttgcgctac acctcgcagc tcgatttgct
     1141 gccttttggt cagttatcca ttgaagaaca agaaaatcca cagcacaggc aaacccggct
     1201 gagcgatatt tgctccggct tacagcaact aaaagccagc gggcgttacc agtggatttt
     1261 aatcgactta ccgcgtgatg cctcgcagat aacccaccag ctgctgagtt tgtgcgatca
     1321 ctcgctggca atcgtcaatg tggatgccaa ctgccatatc cgactgcatc agcaagcgct
     1381 gccggatggc gcacatattt tgattaatga cttccgtatt ggcagtcagg ttcaggacga
     1441 tatttaccag ctttggttgc aaagccagcg ccgattactg ccgatgctca ttcatcgtga
     1501 tgaagcgata gctgaatgcc tggcggctaa gcaaccagta ggtgaatatc gcagtgatgc
     1561 gctggcggct gaagagatac tgacgctggc gaactggtgc ctgttgaact actccgggct
     1621 gaaaacgcca gtcgggagtg catcatgagt atcctgaccc ggtggttgct tatcccgccg
     1681 gtcaacgcgc ggcttatcgg gcgttatcgc gattatcgtc gtcacggtgc gtcggctttc
     1741 agcgcgacgc tcggctgttt ctggatgatc ctggcctgga tttttattcc gctggagcac
     1801 ccgcgctggc agcgtattcg cgcagaacat aaaaacctgt atccgcatat caacgcctcg
     1861 cgtccgctgg acccggtccg ttatctcatt caaacatgct ggttactgat cggtacatcg
     1921 cgcaaagaaa cgccgaaacc gcgcaggcgg gcattttcag gtctgcagaa tattcgtgga
     1981 cgttaccatc aatggatgaa cgagctgcct gagcgcgtta gccataaaac acagcatctt
     2041 gatgagaaaa aagagctcgg tcatttgagt gccggggcgc ggcggttgat cctcggtatc
     2101 atcgtcacct tctcgctgat tctggcgtta atctgcgtta ctcaaccgtt taacccgctg
     2161 gcgcagttta tcttcctgat gctgctgtgg ggtgtagcgc tgatcgtacg gcggatgccg
     2221 gggcgcttct cggcgctaat gttgattgtg ctgtcgctga ccgtttcttg ccgttatatc
     2281 tggtggcgtt acacctctac gctgaactgg gacgatccgg tcagcctggt gtgcgggctt
     2341 attctgctct tcgctgaaac gtacgcgtgg attgtgctgg tgctcggcta cttccaggtc
     2401 gtatggccgc tgaatcgtca gccggtgcca ttgccgaaag atatgtcgct gtggccgtcg
     2461 gtggatatct ttgtcccgac ttacaacgaa gatctcaacg tggtgaaaaa taccatttac
     2521 gcctcgctgg gtatcgactg gccgaaagac aaactgaaca tctggatcct cgatgacggc
     2581 ggcagggaag agtttcgcca gtttgcgcaa aacgtggggg tgaaatatat cgcccgcacc
     2641 actcatgaac atgcgaaagc gggcaacatc aacaatgcgc tgaaatatgc caaaggcgag
     2701 ttcgtatcga ttttcgactg cgaccacgta ccaacgcgat cgttcctgca aatgaccatg
     2761 ggctggttcc tgaaagagaa acagctggcg atgatgcaga cgccgcacca cttcttctcg
     2821 ccggacccgt ttgaacgcaa cctggggcgt tttcgtaaaa cgccgaacga aggcacgctg
     2881 ttctatggtc tggtgcagga tggcaacgat atgtgggacg ccactttctt ctgcggttcc
     2941 tgtgcagtaa ttcgtcgcaa accattggat gaaattggcg gtattgctgt cgaaacggta
     3001 accgaagatg cgcacacgtc tctgcgcctg caccgtcgtg gctacacctc tgcatatatg
     3061 cgtattccgc aggcggcggg gctggcgacc gaaagtctgt cggcgcatat cggtcagcgt
     3121 attcgctggg cacgcgggat ggtgcaaatc ttccgtctcg ataacccgct taccggtaaa
     3181 gggctgaaat tcgcccagcg gctgtgctac gtcaacgcca tgttccactt cttgtcgggc
     3241 attccacgac tgatcttcct gactgcgccg ctggcgttcc tgctgcttca tgcctacatc
     3301 atctatgcgc cagcgttgat gatcgccctg ttcgtgctgc cgcatatgat ccatgccagc
     3361 ctgaccaact cgaagatcca gggcaaatat cgccactctt tctggagtga aatctacgaa
     3421 acggtgctgg cgtggtatat cgcaccaccg acgctggtgg cgctgattaa cccgcacaaa
     3481 ggcaaattta acgtcaccgc caaaggtgga ctggtggaag aagagtacgt cgactgggtg
     3541 atctcgcggc cctacatctt ccttgttctg ctcaacctgg tgggtgttgc ggtcggcatc
     3601 tggcgctact tctatggccc gccaaccgag atgctcaccg tggtcgtcag tatggtgtgg
     3661 gtgttctaca acctgattgt tcttggcggc gcagttgcgg tatcggtaga aagcaaacag
     3721 gtacgccgat cgcaccgcgt ggagatgacg atgcccgcgg caattgcccg cgaagatggt
     3781 cacctctttt cgtgtaccgt tcaggatttc tccgacggtg gtttggggat caagatcaac
     3841 ggtcaggcgc agattctgga agggcagaaa gtgaatctgt tgcttaaacg cggtcagcag
     3901 gaatacgtct tcccgaccca ggtggcgcgc gtgatgggta atgaagttgg gctgaaatta
     3961 atgccgctca ccacccagca acatatcgat tttgtgcagt gtacgtttgc ccgtgcggat
     4021 acatgggcgc tctggcagga cagctacccg gaagataagc cgctggaaag tctgctggat
     4081 attctgaagc tcggcttccg tggctaccgc catctggcgg agtttgcgcc ttcttcggtg
     4141 aagggcatat tccgtgtgct gacttctctg gtttcctggg ttgtatcgtt tattctgcgc
     4201 cgcccggagc ggagcgaaac ggcacaacca tcggatcagg ctttggctca acaatgatga
     4261 taacgcgatg aaaagaaaac tattctggat ttgtgcagtg gctatgggga tgagtgcgtt
     4321 cccctctttc atgacgcagg cgacgccagc aacgcaacca ctgatcaatg ctgagccagc
     4381 tgtagccgcc cagacggaac aaaatccgca ggtggggcaa gtgatgccgg gcgtgcaggg
     4441 cgctgatgcg ccagtcgtgg cgcagaacgg tccttcgcgt gatgtgaagc tgacctttgc
     4501 gcaaattgcg cctccgccgg gcagcatggt gctacgtggc attaacccga acggcagcat
     4561 tgagtttggt atgcgcagcg atgaagtggt gacgaaggcg atgctcaacc tcgaatacat
     4621 gccatcgcca tcgttactgc ctgtccagtc gcagttaaag gtttatctca atgatgagct
     4681 gatgggcgta ctgccagtga ccaaagaaca gttgggtaaa aaaacgctgg cgcaaatgcc
     4741 cattaaccca ctgtttatta ccgacttcaa ccgtgtgcgg ctggagtttg tcggccatta
     4801 tcaggacgtg tgcgaaaacc cggccagcac cacgctttgg ctggatgttg ggcggagcag
     4861 tggactggat ctgacctatc agaccctgaa tgtgaagaat gacctgtcac acttcccggt
     4921 gccattcttt gacccgcgcg ataaccgtac taacaccttg ccgatggtct ttgcgggtgc
     4981 gccggatgtt gggctgcaac aagcatctgc cattgtcgcc tcgtggtttg gttcgcgttc
     5041 tggctggcgt gggcagaact tcccggtgct ctataaccaa ctgccggatc gtaatgccat
     5101 tgtctttgca accaacgaca aacggccgga cttcctgcgc gatcatccgg cggtaaaagc
     5161 cccggtgatt gagatgatca accatccgca gaatccttac gtcaaactgc tggtggtgtt
     5221 tggtcgtgac gacaaagacc tgttgcaggc agcgaaaggt atcgctcagg gtaacattct
     5281 gttccgtggt gaaagcgtgg tagtgaatga agtgaaaccg ctgctaccgc gtaagccgta
     5341 cgatgcgccg aactgggtac gtaccgatcg tccggtcaca tttggcgaac tgaaaaccta
     5401 tgaagaacag ttacaatcca gcggtcttga gccagcagcg attaacgttt cgctaaacct
     5461 gccgccggat ctctacctga tgcgcagtac cggcattgat atggatatta attaccgcta
     5521 caccatgccg ccggtgaaag acagttcgcg gatggatatc agcctgaata accagttcct
     5581 gcaatccttc aacctgagca gcaaacagga ggcgaaccgc ctgctgctgc ggattccggt
     5641 attacaaggt ttgctggatg gcaaaacaga tgtctctatt ccggcgctga aactgggcgc
     5701 gaccaaccag ctgcgcttcg actttgagta tatgaacccg atgccgggcg gttcggtgga
     5761 taactgtatt accttccagc cggtgcagaa tcatgtggtg attggtgacg actccaccat
     5821 cgacttctcg aagtattacc acttcatccc gatgccggat ctacgcgcct ttgctaacgc
     5881 gggcttccca ttcagccgga tggcggatct gtcgcaaacc atcaccgtga tgccgaaagc
     5941 gcctaacgaa gcacagatgg aaacgttgct gaatactgtt ggttttatcg gcgcacagac
     6001 gggcttcccg gcgattaatc tgacggtgac cgatgatggc agcaccattc agggcaaaga
     6061 tgccgacatc atgatcatcg gtggtatccc ggacaaactg aaagacgata agcagatcga
     6121 cctattggtg caggcgaccg aaagctgggt gaaaacaccg atgcgccaga ccccgttccc
     6181 cggcattgtg ccggacgaga gcgatcgcgc ggcagaaacc cggtcaacgc tgacctcttc
     6241 cggtgcgatg gcggcggtga ttggcttcca gtcgccgtat aacgaccagc gcagcgtgat
     6301 tgcgctgttg gcagatagcc cacgcggtta tgaaatgctt aacgatgcgg tgaacgatag
     6361 cggcaaacgc gccaccatgt tcggttcggt cgcggtgatc cgcgagtccg gtatcaacag
     6421 cctacgtgtt ggcgacgttt attacgtagg tcatctgccg tggttcgagc gcgtgtggta
     6481 tgcgctggca aaccatccga ttctgctggc ggtgctggcg gctatcagtg tgatattgct
     6541 ggcatgggta ctgtggcgtc tgctgcgaat tattagtcgt cgtcgtctta acccggataa
     6601 cgagtaattg aagatgaatg tgttgcgtag tggaatcgtg acgatgctgt tgttggctgc
     6661 ctttagtgtt caggcagcct gtacctggcc tgcctgggag cagtttaaaa aggattacat
     6721 cagtcaggaa gggcgcgtca tcgaccccag cgacgcgcgc aaaatcacca cctccgaagg
     6781 gcaaagttac ggtatgttct ttgccctggc ggctaacgac cgtgcagctt tcgataatat
     6841 tctcgactgg acgcagaaca atctcgctca gggttcttta aaagaacgtt tgcccgcctg
     6901 gctgtggggc aagaaagaga acagtaagtg ggaagtgctg gacagcaatt cggcctccga
     6961 tggtgatgtc tggatggcct ggtcgttgct ggaggcgggg cgtttgtgga aagagcagcg
     7021 ttataccgac atcggcagcg cgttgctaaa acgtatcgcg cgggaggaag tggtgacggt
     7081 gcctgggctg ggttccatgt tgttaccggg caaagtgggt tttgctgagg ataacagctg
     7141 gcgttttaac cccagctacc tgccgccgac gctggcgcag tatttcaccc gctttggcgc
     7201 gccgtggact acgctgcgcg aaaccaatca acgtttattg ctggaaaccg ccccgaaagg
     7261 cttttcgcca gactgggtgc gttatgagaa agacaaaggc tggcagctaa aagccgaaaa
     7321 aacattgatc agcagctacg acgccatccg cgtttacatg tgggtaggca tgatgcctga
     7381 cagcgatccg cagaaagcgc ggatgctcaa ccggtttaaa ccgatggcga cattcactga
     7441 gaaaaacggt tatccgccgg aaaaagtgga tgtggctacg gggaaagcgc agggtaaagg
     7501 accagtcggt ttttctgccg ccatgctgcc ctttttacaa aaccgcgatg cgcaggccgt
     7561 tcagcgtcag cgcgtggccg ataactttcc cggcagcgat gcctattaca actatgtgct
     7621 gaccctgttt ggacaaggct gggatcaaca ccgtttccgc ttctcgacaa aaggtgagtt
     7681 attacctgac tggggccagg aatgcgcaaa ttcacactaa acatattcac gctttccctc
     7741 ggtctggccg tcatgccgat ggtcgaggca gcaccaaccg ctcagcaaca gttgctggag
     7801 caagttcggt taggcgaagc gacccatcgt gaagatctgg tgcaacagtc gttatatcgg
     7861 ctggaactta ttgatccgaa taacccggac gtcgttgccg cccgtttccg ttctttgtta
     7921 cgttagggcg atattgatgg cgcgcaaaaa cagctcgatc ggctgtcgca gttagcgccg
     7981 agttcaaatg cgtataaatc gtcgcggact acgatgctac tttccacgcc ggatggtcgt
     8041 caggcactgc aacaggcacg attgcaggcg acgaccggtc atgcagaaga agctgtggcg
     8101 agttacaaca aactgttcaa cggtgcgccg ccggaaggtg acattgctgt cgagtactgg
     8161 agtacggtgg cgaaaattcc ggctcgccgt ggcgaagcga ttaatcagtt aaaacgcatc
     8221 aatgcggatg caccgggcaa tacgggcctg caaaacaatc tggcgctatt gctgtttagt
     8281 agcgatcgcc gtgacgaagg ttttgccgtc ctggaacaga tggcaaaatc gaacgccggg
     8341 cgcgaagggg cctctaaaat ctggtacggg cagattaaag acatgcccgt cagtgatgcc
     8401 agtgtgtcgg cgctgaaaaa atatctctcg atctttagtg atggcgatag cgtggcggct
     8461 gcgcaatcgc aactggcaga acagcaaaaa cagctggccg atcctgcttt ccgcgctcgt
     8521 gcgcaaggtt tagcggcggt ggactctggt atggcgggta aagccattcc cgaactacaa
     8581 caggcggtgc gggcgaaccc gaaagacagt gaagctctgg gggcgctggg ccaggcgtat
     8641 tctcagaaag gcgatcgcgc caatgcagtg gcgaatctgg aaaaagccct cgcactggac
     8701 ccgcacagca gcaacaacga caaatggaac agtctgctga aagtaaaccg ctactggctg
     8761 gcgatccagc agggcgatgc tgcgctgaaa gccaataatc ctgaccgggc agaacgcctg
     8821 ttccagcagg cgcgtaatgt cgataacacc gacagttatg cagtgctggg gctgggcgat
     8881 gtggcgatgg cgcgaaaaga ttatcccgcc gccgaacgtt attatcagca gaccttgcgt
     8941 atggacagcg gcaacactaa cgccgtgcgc gggctggcaa atatttaccg ccagcaatcg
     9001 ccagaaaaag ctgaagcgtt tatcgcctcg ctctctgcca gtcagcggcg tagcattgat
     9061 gatatcgaac gcagcctgca aaacgaccgt ctggcacagc aggcagaggc actggaaaac
     9121 cagggcaaat gggcgccggc ggcagcactt cagcggcaac gactggcgct ggaccccggc
     9181 agcgtatgga ttacttaccg actttcgcag gatctctggc aggccggaca acgcagccag
     9241 gccgatacgt taatgcgcaa tctggcgcag cagaagtcga acgacccgga gcaggtttac
     9301 gcttacgggc tgtacctctc tggtcatgac caggacagag cggcgctggc gcatatcaac
     9361 agcctgccgc gcgcgcagtg gaacagcaat attcaggagc tggttaatcg actgcaaagc
     9421 gatcaggtgc tggaaaccgc taatcgcctg cgagaaagcg gcaaagaagc tgaagcggaa
     9481 gcgatgctgc gtcagcaacc accttccacg cgtattgacc tcacgctggc tgactgggcg
     9541 cagcagcgac gtgattacac cgccgcccgc gctgcatatc agaatgttct gacgctggag
     9601 ccaactaacg ccgatgccat tctggggctg acggaagtgg atattgctgc cggtgacaca
     9661 gcggcggcac gtagccagct ggcgaaactg cccgctaccg ataacgcctc gctgaacaca
     9721 cagcggcgcg tggcgctggc gcaggcgcag cttggagata ccgcagcggc gcagcagacg
     9781 tttaataagt tgatcccgca ggcaaaatct cagccaccgt cgatggaaag cgcgatggtg
     9841 ctgcgcgacg gtgcgaagtt tgaagcgcag gcgggcgatc caaagcaggc gctggaaacc
     9901 tacaaagacg ccatggtcgc atccggtgtg actacgacgc gtccgcagga taacgacaca
     9961 ttcacccgac tgacccgtaa cgacgagaaa gatgactggc tgaaacgcgg cgtgcgcagc
    10021 gatgcggcgg gcctctatcg ccagcaggat cttaacgtca ccctcgagca cgattactgg
    10081 ggttcgagcg gcaccggtgg ttactccgat ctgaaagcgc acactaccat gttgcaggtg
    10141 gatgcgccgt attctgacgg gcggatgttc tttcgcagtg atttcgtcaa tatgaacgtc
    10201 ggcagtttct ccactaatgc cgatggcaaa tgggatgaca actggggcac ctgtacatta
    10261 caggactgta gtggcaaccg cagccagtcg gactccggtg ccagcgtgga ggtcggctgg
    10321 cgaaatgacg tctggagctg ggatatcggt accacgccga tgggcttcaa cgtggtggat
    10381 gtggtcggcg gcatcagtta cagcgatgat atcgggccgc tgggttacac cgttaacgcc
    10441 caccgtcggc ccatctccag ttctttgctg gcctttggtg ggcaaaaaga ctccccgagc
    10501 aataccggga aaaaatgggg tggcgtacgt gccgacggtg tggggctaag tctgagctac
    10561 gataaaggtg aagcaaacgg cgtctgggca tcgcttagtg gcgaccagtt aaccggtaaa
    10621 aatgtcgaag ataactggcg cgtgcgctgg atgacgggct attactataa ggtcattaac
    10681 cagaacaatc gccgcgtcac aatcggcctg aacaacatga tctggcatta cgacaaagat
    10741 ctgagtggct actcactcgg tcagggcggt tactacagtc cgcaggaata cctgtcgttt
    10801 gctataccgg tgatgtggcg ggagcgcacg gaaaactggt cgtgggagct gggggcgtct
    10861 ggctcgtggt cgcattcacg caccaaaacc atgccgcgtt atccgctgat gaatctgatc
    10921 ccgaccgact ggcaggaaga agctgcgcgg caatccaacg atggcggcag cagtcagggc
    10981 ttcggctaca cggcgcgggc attacttgaa cgacgtgtta cttccaactg gtttgttggc
    11041 acggcaattg atatccagca ggcgaaagat tacgcaccca gccatttcct gctctacgta
    11101 cgttattccg ccgccggatg gcagggtgac atggatttac cgccgcagcc gctgatacct
    11161 tacgccgact ggtaagtttt cagatagcgc ctctcttaat gccgctgcga tcgggtatac
    11221 tcgggcggca atctgggatt tccgggggga gacaatttgc gcgtaagtcg ctcgttaaca
    11281 atcaagcaga tggcaatggt ggcagccgtt gtcctggtgt tcgtttttat tttttgcacc
    11341 gttttgctgt tccatctggt ccagcagaat cgctataaca cggctacgca actggaaagc
    11401 attgctcgct ctgtacgcga acccttatct tcagctattt tgaaaggcga tattcccgaa
    11461 gcggaagcta ttcttgccag cattaaaccg gcaggcgtgg tcagccgtgc cgatgtagtg
    11521 ctgcctaacc agttccaggc gctgcgtaaa agttttattc cagagcgccc ggtgccggta
    11581 atggttactc gcctgtttga gctaccggtt caaatctcgc tgggcgttta ctcgctcgaa
    11641 cgtccggcaa acccgcagcc aattgcctat ctggtactac aggcggattc cttccgtatg
    11701 tataagttcg tgatgagcac cctctcaacg ttagtgacca tttacttact tttgtcgctt
    11761 atcctgaccg tcgccatcag ctggtgcatt aaccgcctga ttttgcatcc gttacgcaat
    11821 attgctcgcg aacttaacgc catcccagcc caggagcttg ttggtcacca actggcatta
    11881 ccgcgtctgc atcaggacga tgaaatcggt atgttggtgc gcagttacaa cctcaaccag
    11941 caattgctgc agcgccatta tgaagaacag aacgaaaatg cgatgcgctt cccggtgtcg
    12001 gatttgccga acaaagcctt gctgatggag atgctggagc aggttgtcgc gcgtaaacaa
    12061 accaccgcgc tgatgatcat cacctgtgaa accctgcgtg atactgcggg cgtgctgaaa
    12121 gaggcgcaac gagaaattct gctgctgacg ctggtggaaa aactcaaatc ggtactgtcg
    12181 ccacgtatga tcctcgcgca gattagcggt tatgactttg ctgtcattgc caacggtgta
    12241 caggaaccgt ggcacgcaat caccttaggt cagcaagtgc tcactatcat gagcgagcgc
    12301 ctgccgattg aacgtattca actccgtccg cactgtagca ttggcgtggc gatgttctac
    12361 ggcgatctca ccgccgaaca gctttacagt cgcgctattt ctgcggcatt taccgctcgc
    12421 cataaaggca agaatcagat tcagttcttt gatccgcagc agatggaagc cgcccagaag
    12481 cggttgacgg aagagagcga tatccttaat gcactggaaa atcatcagtt tgctatttgg
    12541 ttacagccac aggtcgagat gaccagcggt aaactggtca gtgcggaagt gttactgcgt
    12601 atccagcaac cggatggcag ttgggacctg ccggatggct taatcgatcg cattgagtgc
    12661 tgtgggctga tggttaccgt cggtcactgg gtgctggaag agtcctgtcg attgcttgca
    12721 gcctggcaag agcgcggcat tatgctgccc ttgtcggtaa acctctctgc gctgcaactg
    12781 atgcacccaa atatggtggc ggatatgctg gaactgttaa cccgctatcg cattcaaccg
    12841 ggaacactga ttctggaagt gacagaaagc cgacgtattg acgaccctca tgctgcggtg
    12901 gcaatcctcc gtccgctgcg caatgccgga gttcgggtgg cgctggatga tttcggcatg
    12961 ggctacgcag ggctgcgtca gctgcagcat atgaaatcgt tgccaatcga cgtactgaaa
    13021 atcgacaaaa tgtttgttga aggcttgccg gaagatagca gcatgattgc tgcaattatc
    13081 atgctggcgc agagcctgaa cttacaaatg attgccgaag gcgtggagac tgaagcacaa
    13141 cgcgactggc tggcaaaagc gggcgttggt attgcccagg gcttcctttt tgctcgccca
    13201 ctccctattg aaatcttcga agagagttac ctggaagaaa agtagctacc ccaaactgat
    13261 tacaaaactt taaaaagtgc tggtttgtgc gagccagctc aaacttttta acctttttgt
    13321 ttcaattatg atccaggtac atttctgtga tgttgtctgg gtgttatttt aaggccgcag
    13381 gtacctcata accttacaag acctgtagtt ttactaaagg acaccctatg aaaacctctc
    13441 tgtttaaaag cctttacttt caggtcctga cagcgatagc cattggtatt ctccttggcc
    13501 atttctatcc tgaaataggc gagcaaatga aaccgcttgg cgacggcttc gttaagctca
    13561 ttaagatgat catcgctcct gtcatctttt gtaccgtcgt aacgggcatt gcgggcatgg
    13621 aaagcatgaa ggcggtcggt cgtaccggcg cagtcgcact gctttacttt gaaattgtca
    13681 gtaccatcgc gctgattatt ggtcttatca tcgttaacgt cgtgcagcct ggtgccggaa
    13741 tgaacgtcga tccggcaacg cttgatgcga aagcggtagc ggtttacgcc gatcaggcga
    13801 aagaccaggg cattgtcgcc ttcattatgg atgtcatccc ggcgagcgtc attggcgcat
    13861 ttgccagcgg taacattctg caggtgctgc tgtttgccgt actgtttggt tttgcgctcc
    13921 accgtctggg cagcaaaggc caactgattt ttaacgtgat cgaaagtttc tcgcaggtca
    13981 tcttcggcat catcaatatg atcatgcgtc tggcacctat tggtgcgttc ggggcaatgg
    14041 cgtttaccat cggtaaatac ggcgtcggca cactggtgca actggggcag ctgattatct
    14101 gtttctatat tacctgtatc ctgtttgtgg tgctggtatt gggttcaatc gctaaagcga
    14161 ctggtttcag tatcttcaaa tttatccgct acatccgtga agaactgctg attgtactgg
    14221 ggacttcatc ttccgagtcg gcgctgccgc gtatgctcga caagatggag aaactcggct
    14281 gccgcaaatc ggtggtgggg ctggtcatcc cgacaggcta ctcgtttaac cttgatggca
    14341 catcgatata cctgacaatg gcggcggtgt ttatcgccca ggccactaac agtcagatgg
    14401 atatcgtcca ccaaatcacg ctgttaatcg tgttgctgct ttcttctaaa ggggcggcag
    14461 gggtaacggg tagtggcttt atcgtgctgg cggcgacgct ctctgcggtg ggccatttgc
    14521 cggtagcggg tctggcgctg atcctcggta tcgaccgctt tatgtcagaa gctcgtgcgc
    14581 tgactaacct ggtcggtaac ggcgtagcga ccattgtcgt tgctaagtgg gtgaaagaac
    14641 tggaccacaa aaaactggac gatgtgctga ataatcgtgc gccggatggc aaaacgcacg
    14701 aattatcctc ttaatctcac cacttatgcc cgcactccct tccccgagtg cgggtgtttg
    14761 cgcataattg acccctgtgc ctgaacttac cacctgggtg agttcaggtt tatttcactt
    14821 cttccgtgac attttcatgt tcttgcggtc taacacgaag tgtttttaac gtcatttaga
    14881 ctgactgaca acgggcagtt ttttattacc aggatagttg atcaggggtt cacatgcagg
    14941 gcacaaaaat tcgactttta gcgggcggtt tgctgatgat ggccactgct ggctatgtgc
    15001 aggcagatgc gctccagcct gatccagcat ggcaacaggg gacgctttcc aacggtttac
    15061 agtggcaagt gctgaccacc ccccagcgtc ccagcgatcg tgttgaaatt cgcctgctgg
    15121 ttaataccgg ttcgctcgcc gaaagtacac aacagagcgg ttacagtcac gccatccctc
    15181 gtattgcgct aacgcaaagc ggtggccttg acgcagcaca ggcgcgttca ttgtggcagc
    15241 aggggatcga ccctaaacgc ccgatgccgc cggtaattgt ctcttatgac accacgctgt
    15301 ttaatctgag tttgcccaat aaccgtaacg atttgctgaa agaagcgctc tcttatctgg
    15361 caaatgccac tggcaaattg accatcacac cagaaaccat caaccacgcg ctgcaaagtc
    15421 aggacatggt ggcaacctgg cctgccgata ctaaagaggg ctggtggcgc tatcgtctga
    15481 aagggtcaac cttgttaggt cacgatcctg ccgatccgct gaaacaaccc gttgaagcgg
    15541 aaaaaattaa agatttctat cagaaatggt acaccccgga tgcaatgacg ctactggtgg
    15601 tgggaaacgt ggatgcgcgc tcggttgtcg accaaatcaa caaaacgttt ggcgaactga
    15661 aaggcaaacg tgaaacgcca gctccggtgc cgacgctttc tccgctgcgt gcggaagcgg
    15721 tgagtattat gactgacgcg gtgcgtcagg accggttatc tatcatgtgg gatacgccgt
    15781 ggcagccgat tcgtgaatca gccgcactgc tgcgctactg gcgtgcggac ctggcccgcg
    15841 aggcgctgtt ctggcatgtt cagcaagcgt taagcgccag taacagcaaa gacatcggtc
    15901 ttggatttga ctgccgtgtg ctgtatctgc gtgcgcagtg tgccatcaac atcgaatcac
    15961 caaacgacaa gctgaacagc aaccttaatc tggtggcgcg tgaactggcg aaggttcgcg
    16021 ataaaggtct gccggaagaa gagttcaatg cgttagtggc gcaaaagaaa ctggagctgc
    16081 agaaactgtt tgccgcctat gcgcgggctg ataccgatat tctgatgggt cagcggatgc
    16141 gttcgttgca aaatcaggtc gtcgatatcg cgccggagca gtatcagaaa ctgcggcagg
    16201 atttccttaa cagcctgacg gtggagatgt taaatcagga tctgcgtcag cagttgtcga
    16261 atgatatggc gttaatactg ctgcagccga aaggcgagcc ggaatttaac atgaaagcgt
    16321 tgcaggcggc ctgggatcaa atcatggccc catcgactgc tgctgccgcc acctctgtcg
    16381 ccacggatga cgtacatcct gaagtgacgg atattccacc tgcacagtaa tgcctgatgg
    16441 tgatgagtta gcacaaccat caggtattta tggatgagct ggatagaggg gtaacacttt
    16501 atccagcctt ttgcatatgc tgcgtttacg ctggcatcgc ctcacgcggg ataatcgcgc
    16561 cgcgatactg aataacggta cttgcggtca ggtgcccgcg tttcgccgcg ttttccgcac
    16621 tgccgcccgt caaacgtacc gccagataac cggcgctgaa agagtcgcca gctgcggtgg
    16681 tatcgatcac tttttctttc ggcagtttca ccgccggaac atccactaac ccttcgccag
    16741 caatggacac caggcaagaa tccgccccgc gtttcaccac cacttctttc acgcccgcgt
    16801 tatgggtgcg cgcaatgacg tcttccaccg gctgttgacc ccacagcgcg tcttcatcgt
    16861 ccagcgtcag gaaggcgata tccgtgcatt ccagcatttg ttggtacacc tgctgtgtct
    16921 cttctttgct ggcccacagg cgcggacgat agttattgtc gaaaatcact tttccgccgt
    16981 tggcgcggca ttcgcgcagc agggaaagca gcttttcgcg gctggtcggg cttaagatcg
    17041 ccaggctaat cccgctcagg tagagataat cgaaattcgc cagctcttcg caaatcgccg
    17101 tagactgctc actctccagc cagaatttgg cggcggcttc gttccgccag tagtagaacg
    17161 tacgctcgcc ggtgctgtcg gtttcaatgt agtaaaggcc cggcagacgg ttttccatcc
    17221 gttgggtcag ggaaatatca acgttctcgc cgtgccaggc gtccagcatc tgctggctaa
    17281 aactgtccgt tcccagcgcc gttacgtaat gaacggttaa tgccgcagga tcgacctgac
    17341 gggcgatata gacggaagtg ttcagggtat cgccgccgaa accgcgctta acgtccgcgc
    17401 ctttctcgga aagctcaatc atgcattcgc caatcacggc aatctttttg gacatagtcg
    17461 tgaacctgat ctgtaaaaaa ttagcgttag tttgcgctgt ggtggtttgc tggtcaacaa
    17521 tattaaaaca gtgttccatt atttttttga gccagaacgt attcctgaaa gattccgttg
    17581 tggagtgagg aaatttaccc cggccaggcg gtaaagttct gcccttacgc gccgataatc
    17641 tttgtcgagt ccgggcagca tcacttttaa acacaggaca tctttgatga taaggcaggt
    17701 tatccagcga ataagcaacc ctgaagcaag catcgagagc ttgcaggaac ggcgtttttg
    17761 gttgcagtgt gagcgtgctt acacctggca gccgatctat caaacatgcg ggcggttaat
    17821 ggccgtggag ctattaacgg tggtcacgca tcccttgaac ccttcgcaac gcctgccgcc
    17881 ggatcgctat tttactgaaa tcaccgtcag ccatcggatg gaggttgtga aagagcagat
    17941 tgatttgctg gcgcaaaaag ccgacttctt tatagagcac ggcctgctgg catcggtcaa
    18001 tattgatggc cctacgctca tcgccctgcg tcagcaacca aaaatcctgc gccaaattga
    18061 gcgtcttccc tggctgcgtt tcgaactggt ggagcatatc cgtctgccga aagattcaac
    18121 ctttgcctcg atgtgtgaat ttggcccgct gtggctgggt gattttggta ccgggatggc
    18181 aaatttctct gcactaagtg aagtgcgtta tgactacatc aaaatcgcgc gagaactgtt
    18241 tgtgatgctg cgtcagtcgc cggaaggacg cacactcttt tctcagcttt tacatctaat
    18301 gaatcgctat tgtcgcgggg tgattgttga gggcgtagaa acgccggaag agtggcgtga
    18361 tgtacagaac tcgcccgcat tcgccgcaca aggctggttt ctttcacgcc cggcaccgat
    18421 agaaacgctg aatacggcgg ttctggcgct ataagctgcc tcattttccg cctggctgga
    18481 ctatctttag gactggcaca ggaagaatat gaggaagcga acgatgagca aggcaggcaa
    18541 aataactgct gcgatttcag gggctttctt gttgttgatt gtcgtggcga tcattttgat
    18601 tgcaactttt gactggaatc gactcaaacc gaccatcaac cagaaagtct ctgcggagtt
    18661 gaatcgtccg ttcgctatcc gtggcgatct gggcgtggtg tgggagcggc aaaaacaaga
    18721 aactggctgg cgcagctggg tgccgtggcc tcatgtacat gcagaagaca tcattcttgg
    18781 caatccaccg gatattcccg aagtcacgat ggtgcatttg ccgcgagtag aggcaacgct
    18841 ggcaccgctg gcgctgctga ccaaaacggt ctggctgccg tggatcaagc tcgaaaagcc
    18901 cgacgcgcgc ctgattcgcc tctccgaaaa gaacaataac tggacgttta atctcgccaa
    18961 cgatgataac aaagacgcga atgcaaagcc gtcggcatgg tcgtttcggc tggataatat
    19021 tcttttcgat caagggcgga tcgccattga tgacaaagta agcaaagcgg atctggaaat
    19081 ttttgttgat ccgttaggca agccgctgcc gttcagcgaa gttactggat cgaaaggtaa
    19141 agcggataaa gaaaaggtgg gcgattacgt ttttggcctg aaggcgcagg gacgttataa
    19201 cggtgaaccg ctcacgggta cgggaaaaat aggcggtatg ctggcgctgc gtggcgaagg
    19261 gacgccgttt ccggtacagg ctgatttccg ttcaggtaat acccgtgttg cttttgatgg
    19321 cgtcgtgaat gacccaatga agatgggcgg tgtcgattta cggcttaaat tttctggcga
    19381 ttcactgggt gatctctatg aactgacggg cgttctgctg cccgataccc cgccgtttga
    19441 aacggatggt cggctggtag cgaaaatcga cactgaaaaa tcgtcggtct ttgattatcg
    19501 cggttttaat gggcgaattg gcgatagcga tatccacggt tctctggtct acaccaccgg
    19561 aaagccgcga ccaaaactgg aaggtgatgt cgagtcgcgg caattgcggc tggcggacct
    19621 gggaccgttg attggcgttg attcggggaa aggggcagaa aagtcgaaac ggtctgaaca
    19681 gaagaagggc gaaaaaagcg ttcagcctgc gggcaaagtg ctgccttatg accgcttcga
    19741 aaccgataaa tgggacgtta tggatgccga tgttcgcttc aaaggccgac gtatcgagca
    19801 tggcagtagc ctgccgatta gcgatctttc tactcatatc atcctcaaaa atgctgacct
    19861 gcgcctgcaa ccgctgaaat ttggcatggc gggcggcagc attgcggcga atatccatct
    19921 ggaaggcgat aaaaagccga tgcaggggcg ggcagatatt caggctcgtc gactgaaact
    19981 gaaagaactg atgcccgatg tggaactgat gcagaagacg ctgggggaaa tgaacggtga
    20041 cgcggaacta cgcggtagcg gtaactcggt ggcggcgctt ttaggcaaca gtaacggcaa
    20101 cctgaaactg ttgatgaatg acgggctggt gagccgcaac ctgatggaga ttgttgggct
    20161 gaatgtcggc aactacattg tcggtgcgat atttggtgac gatgaggtgc gggtgaactg
    20221 cgcggcggcg aatctgaata ttgccaacgg cgtagcgcgc ccgcagattt ttgctttcga
    20281 tactgagaac gcgttgatta atgttaccgg cacggcaagt tttgcttcgg aacagctgga
    20341 tttgactatt gatccggaga gtaaagggat tcggattatc acactgcgtt cgccgctgta
    20401 tgtgcgtggg acgtttaaaa atccgcaggc tggggtgaaa gccggaccgc tgattgcccg
    20461 tggtgccgtt gcggcggcac tggcaacgct ggtaacaccg gcggcggcgt tattggcact
    20521 gatctcacct tccgaagggg aggctaatca gtgtcggacg atattgtcgc agatgaagaa
    20581 gtgatgaggt tggattgtgt ttgctggtga gttcgggagc agcgtgttcc cctcacccta
    20641 atcctctccc caaaggggcg aggagactgt tttgtgcgtg ttgaaacaac gagtaccgct
    20701 tttccgttca ctttctgggg gagggctgtt atgttcgcga tataacatct cgctccgctt
    20761 ttctccctct ccctttcagg gagagggctg gggtgagggt ggctgcccac acgatactta
    20821 caacgactga tgttgcgtct catgggtcag cagcagggcg attaacgtca agccagccat
    20881 cgccgccaga tataacccca ccgcacctaa cccgtagtta gtctgcaacc aggctgcgat
    20941 atacggcgca acggaagccc cgagaatcga cgatacgttg taagagaacg atgctccggt
    21001 gtaacgcact tctgtcggga acagctctgg taacagcgcg cccatcggac cgaaggtcag
    21061 ccccatcaga cttaacccca gcagcaggaa ggcaaaaacc agaatcgggt tgccagaacc
    21121 gagcaaaggg ttaaaggcga atagcgcgaa caggatgagc agcgtggtga tgattaccat
    21181 gcttttacgg cgaccaaagg catcagccag taatccagcg actggcacca tcacgccaaa
    21241 accaataact gccatcatca acatccacaa cacttcgtta cgcggcaggc caagcccaac
    21301 tggcgcggcg gcggtactaa aggtcatgga gtagaccgtc atgatgtaaa acagcgtata
    21361 ggttgccagc ataatgaacg tacccagtac ggttacgcga acatgtttgg tcagcagcgt
    21421 acccagcggg atcttcacct gttttttcgc tttagcgact ttctcaaaca ccggcgactc
    21481 atgcagcgac acgcgaacat acaggccgat aatgaccagc accgccgaga agataaatgg
    21541 cacacgccag ccccagctca taaactgctc gtcggtcagt agccaggaaa gcagcaagaa
    21601 agtgccgttg gcaaagaaga agccaatcgg tgcgcccagc tgcgggaagg agccgtacaa
    21661 tgcacgtttg cgcggtgggg cgttttcagt cgccagcagc gccgcgccgc cccattcacc
    21721 acctaagccc agaccctgac caaatcgagc cagcgccagc agcagcgggg cgaaaatacc
    21781 aatcgtggca tagcctggca gcagaccaat caccacggtc gaaatcccca tcgttagcaa
    21841 cgaggcgacc agcgtcgctt tacgcccaac gcgatcgcca aaatgaccaa aaacggcaga
    21901 gccaatgggg cgcgcgacga aggcgatggc gaaggtggcg agcgactgta gcgttgctgc
    21961 cgtaggatcg ccctgcggga agaagatatg cggaaacaca ataacggccg cagtggcgta
    22021 aatgtaaaag tcgaagaact caatggctgt gccaatgaga gaggcgacaa ggactttatt
    22081 acgcgagttg atcggcgtgt attcttgctc gtggtcgagt gttgtggctg ttgcttgcat
    22141 aatcttttct tattttttgg ctaacgaata gccatattac gcacagcaaa agcgatattt
    22201 caatgtgtaa caaatcgcgg aaatggtcaa aaaccaggca aaaaggggat aatgtttagg
    22261 ccagtgattt ttcttctatg aaatgcttca cataatttta taaatagtgg ataaaactgg
    22321 tttcaggtta aataaaagtt accaactgga tttatatgct ggggttatga gttagaatga
    22381 aattgtttgc tcccaaatag atggctgaag aggctctggg gacgagtttc aagaggttat
    22441 gtgcgcatta tagccggatg cgatgctggc gcatcttatc cggcctacgg ggagccaggc
    22501 cgaataaaat cgtatgccgg ataaggcgtt tacgccgcat ccggcctatt ttaaggctgc
    22561 gttttccctg gcattcgcgg gtcgtcttta tattcggcgg tggcaatcca cgccgcgcaa
    22621 aacagcgtca aacgggcgaa gaagtagaaa aatgccatca gccccagcac ggaaccaaat
    22681 gctgcgccag agggggattt catcagcgat ggcagggtgt aggtcatcac gattttaatc
    22741 acttcaaaac caatagcagc cagaaatgtt ccgcgaatca gggctttttt gcgcggacgg
    22801 tggcgcggca accgccagaa gatccagaag aaaagcagat agttggcgaa gatggaaatc
    22861 gccaggccaa tcaatcgcca cgtcggtttc agccactcaa tgctgttcag gtgcagggcg
    22921 ctaataatca tttgctgcgc cgaaccggca accgaggtga tcgaaagcgt cacaatcagc
    22981 gcaatcaaca aaccaatcag tgaaataaaa tcacgcagat attttaccca gaacttctcc
    23041 tgatcttgcg gcgagcgttc ccagacatca cgcgactggg cacgaatcgc ttcacgcagg
    23101 ttacccatcc agttgatgcc ggaataaagt gccaccgcca ggccgacaag ccctacagtc
    23161 gtacgctgct gaacggcggt gttgatggtg tttttcaacg tggcggctag tgtcggatcg
    23221 ctgatgtttt gcagaatttt gtcgaagata tcctgtagca acatcggatg ggaggccagc
    23281 acaaagcccc ctgcggcaaa cgacaccatc aaaatcggga tcatcgacaa aaatgagaaa
    23341 taggtgatag ccgcaccaaa ctggttaccc agccgatcgt taaagcgttc tgtcgcgcga
    23401 atcaggtgcg caataaccgg ttggcgttgg actttttcgg cagtggtggt gacggtttcc
    23461 agcgcctggc taactttgcc gcctttctcg ctattatcca actgcttaat cggctcgtgc
    23521 tccagatcct gggtgggacg tttgatctcg ttttcctgcg tcatccgctc ttttgtcctt
    23581 ctgtcgtggt cattcttaaa aagtatagtc ggtcagtcca ggtgctcttt cattacgcca
    23641 gccagccact gcataaacag gtttacacgc cgggaaagct cccgacgctg cgggtaaacc
    23701 agcgaaaggg agagcggctc ggcacggtag ccaggtaata cttcaataag ccgcccggca
    23761 cgcagggctt cgcgcacggc aatgcgcggg atctgaataa tccccagacc ggcaagaccg
    23821 gcggcgagat agttttcgct actatttacc gtcaacatgc cgccagactt aaaccactgg
    23881 acgccattaa cgctggcaac ctcaaaacct aacggatgta cacccaggtg cggtgtataa
    23941 cgcactattg cgtgtgaagt cagatcatcg gggctttgcg gataaccaaa gcgcgtcaga
    24001 tagtgcggac tggcacagtt gaccatggtc agtttgccga ggggacgggc gataacgcca
    24061 tcttccggta acgcgccagt gcgtatcacg caatcaaaac catcatgaag aatatcgacc
    24121 ggacggtcat ggctactcag ttccagctca attcccggat gcagatagag aaattccgac
    24181 aggcgcggca gtaacaggtt tttcgcgatt cctggcggga tgtcgatgcg taatttaccg
    24241 ctgatactgg tggcatcctg ttgaaacaga ccgtccagtt cgttgagatt actcaacaca
    24301 tcttttgccc gttgataata ggtcatgcct tctggcgtga gtttgacccg tcgcgtggtg
    24361 cgctggagaa gctgggtgcc aagttgatgt tccagtgcct gtatctggcg cgaaacactt
    24421 ccctttggca aagcaaagaa atccgctgcg cgggaaaaac tttccagctc cgcgactttg
    24481 atgaacaact gcattgcgtg aattttatcc atttctttga ccgattgttg ttcacagcga
    24541 aacagtaacg cgcatatcgc attgtttatt gttttttcta acatctaatg ccgggtttca
    24601 caatgttgtc atgacgaatg tggcgatttc tcagcaggaa aagggcggaa tagctttatg
    24661 agaaaggtga atccccttaa acagagcgta tttttcagtg attcccttta aagtgaatag
    24721 aggacgtatt ggctatttgt gcttattttt gtctcattgc catttgttat tttggcttgt
    24781 agtcgcggaa tttcattttt aattgcatgc gtactaacca ccaatataag gaaatcctca
    24841 aaattgtttt aaatagtgcc ctcgacatgt aatttcaggc gtttagctgt aactatttat
    24901 atcggtaact attttctacg cgtcatctct gtagaatggc caccgccaaa tggcgaattc
    24961 ttattttttc tcgcgggtaa tacccttcca ctacttaagt ctttttaaca tattcaggtg
    25021 gagattcagt tatgcaaata gtcatgtttg acaggcagtc aatatttatt catggaatga
    25081 aaatcagttt acagcagcgt attccagggg tgagtattca gggggccagt caggcagacg
    25141 agttatggca aaagctggaa agttaccctg aagccttagt tatgctcgat ggcgatcagg
    25201 atggtgagtt ttgctactgg ttgctgcaaa aaaccgtggt gcagtttcct gaggttaagg
    25261 tgttaatcac ggcgacggat tgcaataaac ggtggttaca ggaagttatc cattttaatg
    25321 tgctggccat tgtgcctcgt gattcaaccg tcgagacatt tgcgttggcg gtaaatagtg
    25381 cggcgatggg gatgatgttt ctgcctggag actggcggac gacgccggaa aaggacatta
    25441 aagatctcaa atcgttaagc gcccgccagc gggagatttt aaccatgtta gcggcgggcg
    25501 agtcaaataa ggagatcggc agagcgctga atatcagtac cggaacggta aaagcgcatc
    25561 tggaatccct gtatcgccgt ctggaagtga aaaatcgcac ccaggcggca atgatgttaa
    25621 atatctcctc ctg
//
LOCUS       DADLFA010000040        25509 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000040 DADLFA010000000
VERSION     DADLFA010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25509
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.040"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..183
                     /locus_tag="JQH65_001404"
     CDS             <1..183
                     /locus_tag="JQH65_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HAY6138872.1"
                     /translation="ACMAITNLTAILLLSPVVHTIASDYLRQRKLGVRPVFDPLRYPD
                     IGRQLSPDAWDDVSQE"
     gene            253..1029
                     /gene="yaaA"
                     /locus_tag="JQH65_001405"
     CDS             253..1029
                     /gene="yaaA"
                     /locus_tag="JQH65_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HAY6138873.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(1182..1478)
                     /locus_tag="JQH65_001406"
     CDS             complement(1182..1478)
                     /locus_tag="JQH65_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HAY6138874.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGNRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(1692..2978)
                     /gene="thrC"
                     /locus_tag="JQH65_001407"
     CDS             complement(1692..2978)
                     /gene="thrC"
                     /locus_tag="JQH65_001407"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HAY6138875.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(2979..3911)
                     /gene="thrB"
                     /locus_tag="JQH65_001408"
     CDS             complement(2979..3911)
                     /gene="thrB"
                     /locus_tag="JQH65_001408"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HAY6138876.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(3913..6375)
                     /gene="thrA"
                     /locus_tag="JQH65_001409"
     CDS             complement(3913..6375)
                     /gene="thrA"
                     /locus_tag="JQH65_001409"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HAY6138877.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTSGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGACRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     gene            complement(6455..6520)
                     /gene="thrL"
                     /locus_tag="JQH65_001410"
     CDS             complement(6455..6520)
                     /gene="thrL"
                     /locus_tag="JQH65_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HAY6138878.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(6734..7420)
                     /locus_tag="JQH65_001411"
     CDS             complement(6734..7420)
                     /locus_tag="JQH65_001411"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HAY6138879.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLVESLHDVDFTVATTARSRAKYHYYATPVELVLLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPTKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(7820..7960)
                     /locus_tag="JQH65_001412"
     CDS             complement(7820..7960)
                     /locus_tag="JQH65_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6138880.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            8056..8772
                     /gene="arcA"
                     /locus_tag="JQH65_001413"
     CDS             8056..8772
                     /gene="arcA"
                     /locus_tag="JQH65_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HAY6138881.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(8832..10184)
                     /gene="creD"
                     /locus_tag="JQH65_001414"
     CDS             complement(8832..10184)
                     /gene="creD"
                     /locus_tag="JQH65_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HAY6138882.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMPLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDWATKYVILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTMDDQLRIWK
                     "
     gene            complement(10242..11666)
                     /gene="creC"
                     /locus_tag="JQH65_001415"
     CDS             complement(10242..11666)
                     /gene="creC"
                     /locus_tag="JQH65_001415"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HAY6138883.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDSQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTVVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVALKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(11666..12355)
                     /gene="creB"
                     /locus_tag="JQH65_001416"
     CDS             complement(11666..12355)
                     /gene="creB"
                     /locus_tag="JQH65_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HAY6138884.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QLVPDVMILDVGLPDISGFELCSQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLHAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(12368..12841)
                     /gene="creA"
                     /locus_tag="JQH65_001417"
     CDS             complement(12368..12841)
                     /gene="creA"
                     /locus_tag="JQH65_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HAY6138885.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            13052..13921
                     /gene="robA"
                     /locus_tag="JQH65_001418"
     CDS             13052..13921
                     /gene="robA"
                     /locus_tag="JQH65_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HAY6138886.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(13918..14565)
                     /gene="gpmB"
                     /locus_tag="JQH65_001419"
     CDS             complement(13918..14565)
                     /gene="gpmB"
                     /locus_tag="JQH65_001419"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HAY6138887.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAARRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            14617..15138
                     /gene="yjjX"
                     /locus_tag="JQH65_001420"
     CDS             14617..15138
                     /gene="yjjX"
                     /locus_tag="JQH65_001420"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP phosphatase"
                     /protein_id="HAY6138888.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(15223..15549)
                     /gene="trpR"
                     /locus_tag="JQH65_001421"
     CDS             complement(15223..15549)
                     /gene="trpR"
                     /locus_tag="JQH65_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HAY6138889.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(15639..17576)
                     /gene="sltY"
                     /locus_tag="JQH65_001422"
     CDS             complement(15639..17576)
                     /gene="sltY"
                     /locus_tag="JQH65_001422"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HAY6138890.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            17787..19454
                     /gene="ettA"
                     /locus_tag="JQH65_001423"
     CDS             17787..19454
                     /gene="ettA"
                     /locus_tag="JQH65_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HAY6138891.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(19761..20993)
                     /locus_tag="JQH65_001424"
     CDS             complement(19761..20993)
                     /locus_tag="JQH65_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide
                     kinase/transcriptional regulator NadR"
                     /protein_id="HAY6138892.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(21014..22396)
                     /gene="radA"
                     /locus_tag="JQH65_001425"
     CDS             complement(21014..22396)
                     /gene="radA"
                     /locus_tag="JQH65_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAY6138893.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(22445..23413)
                     /gene="serB"
                     /locus_tag="JQH65_001426"
     CDS             complement(22445..23413)
                     /gene="serB"
                     /locus_tag="JQH65_001426"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HAY6138894.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            23519..24163
                     /locus_tag="JQH65_001427"
     CDS             23519..24163
                     /locus_tag="JQH65_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HAY6138895.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            24191..25207
                     /gene="lplA"
                     /locus_tag="JQH65_001428"
     CDS             24191..25207
                     /gene="lplA"
                     /locus_tag="JQH65_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HAY6138896.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAEIFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
BASE COUNT         6131 a         7029 c         6335 g         6014 t
ORIGIN      
        1 gcctgcatgg ctattaccaa tttgaccgcc attttactgc tctcgcctgt ggttcatacc
       61 attgccagtg attatctacg ccagcgtaaa ctcggcgtgc gcccggtgtt tgatccgttg
      121 cgttatccgg atatcggtcg ccagctttct ccggacgcgt gggatgatgt ttcgcaggag
      181 taatcacaac tatcgatcaa ctcattctct ttttttgcta aagtcggcat aaatttcctg
      241 caaggactgg atatgctgat tcttatttca cctgcgaaaa cgcttgatta ccaaagcccg
      301 ttgaccacca cgcgctatac gctgccggag ctgttagaca attcccagca gttgatccat
      361 gaagcgcgga aactgacgcc tccgcagatt agcacgctga tgcgcatcag cgacaaactg
      421 gcgggtatca acgccgctcg ctttcatgac tggcagccag atttcacgcc ggcgaatgcc
      481 cgccaggcga ttctggcgtt taaaggtgat gtctacaccg gcttgcaggc cgaaaccttc
      541 agcgaagacg atttcgattt tgcccaacag catttgcgaa tgctttccgg cttgtatggc
      601 gtactgcgcc cgctcgattt aatgcagcct tatcgtctgg aaatggggat ccgtcttgag
      661 aatgcccgag ggaaagatct gtatcaattc tggggagata tcatcaccaa caagctgaac
      721 gaggcgctcg cagcacaagg cgataatgtg gtgattaatc tggcgtcaga tgagtatttt
      781 aaatcggtga agccgaagaa attgaatgcc gagattatca agccggtgtt cctcgatgag
      841 aagaacggca aatttaagat catcagcttc tacgctaaga aagcacgcgg tctgatgagt
      901 cgtttcatta ttgaaaatcg gctgaccaaa ccagagcaac tgactggttt taatagcgaa
      961 ggttacttct ttgatgaagc ttcctccagc aatggcgaac tggtgtttaa acgctacgag
     1021 cagcgttaag tttgcagtca atgccggatg cggcgtgaac gccttatccg gcctacaaaa
     1081 gcatgcagat tcaatatatt gcagaattca cgtaggcctg ataagacgcg ccagcgtcgc
     1141 atcaggcgct gaatgccgga ttgttacccg gcatttgtca tttagcggtg atgtttgcct
     1201 ggaccatgac cgccgtgatg atcatgagga gctttcttat ggtggcgcgg cggtggcggc
     1261 ggtccgtgtg ggtgccagcg attgcctcgc cattcataat gttgtttcca ccagccgtgg
     1321 tcgcgccagt gacctccatc ccagtaatag ccacgattat cacgattgcc tatctgtaat
     1381 tttactgacg ggactaacgt aatttccgca gcctgtgctg ccatgggagc gaccagaacc
     1441 agggaaagtg cgagtacgat agattgcatc tttttcacag gttactcctt ttatcattgc
     1501 ccgttatggg tcgatatccg gagattacgc gatgagaacg acggttgtta ttctccgcaa
     1561 tcctaatctc taagttaccg catttattga gaatatctcc tttttccctg tcgtttttct
     1621 ccataatttc ttcataaaaa agccgggctg cataaaagca aacccggcct gattgagata
     1681 atgaatagat tttactgatg attcatcatc aatttacgca acgcagcaaa atcggcgggc
     1741 agattatgtg aaagcaaggg taaatcagca cgttctgcca gctcttttgg cagatccaac
     1801 gtttcaccga gaatcgcttc cacgctctct ttaaatttcg ccggatgcgc ggtgccgagg
     1861 aacaagccat attcgcctgg attcaactgg tcacgcagcg cacgatacgc tacggcagcg
     1921 tgcggctccg aggtgtagcc cagttctttt aactcacgca ttgtctgttg cgtggtttca
     1981 tcatccacgg ctgcataacc cagctctttc agttgccaga ttttgcggcg gaacaactct
     2041 tccacacgcg gccagttgtt cggctgactc acgtccatcg cgttggataa cgtcgcctga
     2101 gtcgctttgg gtgaccactg accgtcgtgc aggaaacgtg gcacggtatc gttcacgttg
     2161 gtcgcagcaa taaaacgttt caccggcaga ccgagtgact tcgccagcag acccgccgtc
     2221 aaatcgccga agtttccgct tggcaccgag acaaccagct ggttgcgcgc ttcctgcggc
     2281 agctgcgcaa ctgcttcaaa gtagtagcaa atctgcgcca gcaaacggct gatgttaatc
     2341 gagttagctg agtttaaccc cagcgccact ttcagctctt catcatcaaa cgcctgcttc
     2401 accagcgcct gacaggcatc gaaatcgccg tcgatggcaa cagtttcgat attgccgccc
     2461 aatgtacaga acagtttttc ttgcagtgga ctgattttgc ctcgtggata gaggataacc
     2521 actttcacat tcggtaaacc gtagaaagca tgagccactg ccgctccggt atcaccggag
     2581 gtcgcggtca gaatggtcac tggcttatcg cccgcaatat gggtcagcat ttgtgccata
     2641 aagcgaccgc cgaaatcttt aaatgccagc gttggcccgt ggaacaattc cagacaaccg
     2701 acatcgcttt caacattggc gaccggagcc gggaaggcaa acgccgcgcg cacgcgctct
     2761 tccaggattt cctgcgggat ttcatcacca ataaacgccg agaggatctt cgcactgcgg
     2821 gtgacaaaat ccagcttcag catctcatca atttcagtca ggctgaattc cggcaggtcg
     2881 tgcggaaaaa acagcccctg atttttgccc aacccctggg ttacggcttg cgcaaagctg
     2941 acctgctcat tgtgatcttt cagattgtag agtttcattt agttttccag tactcgtgcg
     3001 cccgccgtat ccagccggca aatatgaaca aaaccttcct gattttgcag gtagttctta
     3061 cccaaccagt cggcaacgcg ctgggcggta tccggcttgt cacacagagc gaacaaggtc
     3121 gggccggagc cggagatacc gctcgctacc gcgccgattt ccgcaaccgc ctgccgcgcc
     3181 tgccggaagc caggcagtaa ccgttcacgg tagggttcag cgataacatc tttcatcagc
     3241 ttcgcggcaa gctcaggctg acgggaatag caggcgtgaa tgaagccagc cagatgtcgc
     3301 ccgtgcgcaa tgcaatcctg gcggcgatac tgcgccggta aaatagccct ggcttctgcc
     3361 gttgagactt taatccccgg atacgccagc acccacagcc actcatcaaa ccctggcact
     3421 tgctggctga tgatgtcgtt ttcttcgatc atcaactgca taccaccaag aaaacacggt
     3481 gccacgttgt cgtaatgaat gctgccggag attcgtcctt ccagctcgcc catcaaagcc
     3541 agcaaacgag tgtcattaag cggcttgcca cagtgttcat tcatcgccat cagcgccgcg
     3601 accaccgaac aggcgctgga gcctaagccc gaaccgattg gcatattctt ttccagagtc
     3661 atcgccactg gaatttgctt gccaagctcc tggcaaaaac gctcccagca ctgataaacg
     3721 atattttccc gtggttctga cggcagctta tcggcaaagc gtccgaggtt gttgagactg
     3781 aatgtctctg ccgcctcaac cgtgactaca tctccgagca atgcaccatc aacaggtgtc
     3841 accgccgccc cgagcacatc aaacccgacg ctcatattgg cactggaagc cggggcataa
     3901 actttaacca tgtcagactc ctaacttcca tgagagggta cgtagcagat cggcaaagac
     3961 accggcagct gtaacgtcat tgcccgcacc atatccgcgc agcaccaacg gcagcggctg
     4021 ataatagtgg ctataaaagg ccagggcgtt ttcgccattt ttcactttga acagcggatc
     4081 attaccatcc acttcggcaa tcttcacgcg gcaggcacca tcttcatcaa tattgccaac
     4141 atagcgcaaa acttttcctt catcacgggc cttcgccacg cgcgcggcaa agagatcgtc
     4201 gagctgtgac agattcgcca taaaagcggc aacatcaccc tcagcgttaa actctgcggg
     4261 cagcacaggt tcaatttcaa tatccgccag ctccagttca cgtcccgttt cacgagcgag
     4321 aatcaatagc ttacgcgcta catccatacc agaaagatca tctcgcggat ccggttcggt
     4381 ataacccatt tcccgcgcca gagtagtcgc ctcggagaaa ctcatgcctt cgtctaactt
     4441 gccgaagata taagaaagcg aacctgaaag aatgccggag aacttcatca attcatcacc
     4501 agcattgagc agattttgca ggttctcaat aaccggtaat ccagccccaa cgttggtgtc
     4561 atagaggaat ttacgccgcg atttttccgc cgcatgacgc aacagatggt agtaatccat
     4621 cgacgaggtg ttggcctttt tgttcggcgt gacaacgtgg aaaccttcgc gcaagaagtc
     4681 ggcatattga tccgccactg cctggctgga ggtgcagtca acaatgaccg ggttcagcag
     4741 atgatattct ttcacgaggc gaattaagcg cccgagatta aacggctctt tggcttgcgc
     4801 cagttcttcc tgccagtttt ccagatttag gccatgcaca ttggtgagca gtgccttcga
     4861 gttggcaaca ccgcagacac gtaagtcgat atgtttattc ttcagccagc tttgttgacg
     4921 cttcagttgc tccagcagcg caccgccaac gccaccgacg ccaatcacaa acacttcgat
     4981 aacctgatcg gtattgaaca gcatctgatg agtaacgcgc acgccagtgg tcgcatcatc
     5041 gttatttacc acgacagaga ttgagcgttc agaagatccc tgagcaatgg cgacaatgtt
     5101 gatattggcg cgggccagcg cggcaaagaa tttcgccgag atcccacgca aggtgcgcat
     5161 accatcacct accaccgaga taatggccag ccgttccgtc accgccagcg gctccagtaa
     5221 gccttctttc agttccaggt agaactcttc ctgcattgcc cgttcagctc gcacacagtc
     5281 gctttgtgga acgcagaaac tgatactgta ttcggaagat gattgcgtaa tcagcaccac
     5341 ggaaatacgg gcgcgtgaca ttgcagcaaa gacgcgcgcc gccatgccga ccattccttt
     5401 catccccgga ccagaaacgc tgaacattgc catattattc agattggaaa tgcctttgac
     5461 cggtaattcg tcttcatcac ggctggcacc aatgagcgta cctggtgctt gaggatttcc
     5521 ggtattttta atcaggcaag ggatctggaa ctgggcgatg ggggtaatgg tgcgggggtg
     5581 aagaacttta gcgccgaagt aggaaagctc catcgcttcc tggtaggaca tcgacttcaa
     5641 caacctcgca tcgggcacct gacgcgggtc gcaggtatag accccgtcaa cgtccgtcca
     5701 aatctcgcaa caatcggcgc gtaaacaggc agccagcacc gccgcggagt agtcggaacc
     5761 gttacgtcca agtaccacca gttcgccttt ttcattaccg gaggtgaaac ctgccatcaa
     5821 caccatgtga tcagccggaa tgcggcttgc cgcaatacgg cgggtggact cagcaatatc
     5881 gacggtagat tcgaggtaat gccccactgc cagcagtttt tcgaccggat cgataacggt
     5941 aacgttgtga ccacgagctt ctaacacgcc ggccataatg gcgatcgaca ttttctcgcc
     6001 acggcaaatc agcgcagcgt tgatgctatc cgggcactgc cccaacaaac taatgccatg
     6061 caggacatgt tttatttggg caaattcctg atcgacgaaa gttttcaatt gcgccagcgg
     6121 gaaccccggc tgggcggcgg cgagtcccgt caaaagttcg gcaaaaatac gttcggcatc
     6181 gctgatattg ggtaaagcat cctggccgct aatggttttt tcaatcatcg ccaccaggtg
     6241 gttggtgatt ttggcggggg cagagaggac ggtggccacc tgcccctgcc tggcattgct
     6301 ttccagaata tcggcaacac gcagaaaacg ttctgcattt gccactgatg taccgccgaa
     6361 cttcaacact cgcatggttg ttacctcgtt acctttggtc gaaaaaaagc ccgcactgtc
     6421 aggtgcgggc ttttttctgt gtttcctgta cgcgtcagcc cgcaccgtta cctgtggtaa
     6481 tggtgatggt ggtggtaatg gtggtgctaa tgcgtttcat ggatgttgtg tactctgtaa
     6541 tttttatctg tctgtgcgct atgcctatat tggttaaagt atttagtgac ctaagtcaat
     6601 aaaattttaa tttactcacg gcaggtaacc agttcagaaa ctgctatcag acactctttt
     6661 tttaatccac acagagacat attgcccgtt gcagtcagaa tgaaaagctg aaaaatactt
     6721 actaaggcgt tttttatttg gtgatatttt tttcaatatc atgcagcaaa cggtgcaaca
     6781 ttgccgtgtc tcgttgctct aaaagcccca ggcgttgttg taaccagtcg accagtttta
     6841 tgtcatctgc cactgccaga gtcgtcagca atgccatgac tcgttcgcgt aaagcttgca
     6901 gttgatgttg gtctgccgtt gtgtcacttt tcgtcggttg ttgtattaat gttgctaatt
     6961 gatagcaata gaccatcacc gcctgcccca gattgagcga aggataatcc gccaccatcg
     7021 gcacaccagt aagaacgtca gccaacgcta actcttcgtt agtcagcccg gaatcttcgc
     7081 gaccaaacac cagcgcggca tggctcatcc atgaagattt ttcctctaac agcagcacca
     7141 gttctactgg cgtggcgtag taatgatatt tcgcccgact gcgcgcagtg gtggcgacag
     7201 tgaaatcgac atcgtgtaac gattcaacca gtgtcgggaa aactttaata ttatcaataa
     7261 tatcaccaga tccatgtgcg acccagcgag tggctggctc cagatgtgcc tgactatcga
     7321 caatccgcag ttcgctaaaa cccatcgttt tcattgcccg cgccgctgcc ccaatatttt
     7381 ctgctctggc gggtgcgacc agaataatcg ttatacgcat attgccactc ttcttgacca
     7441 aatgaccgcg aacagggtga tcactgtcaa cttattacac ggtgcgaatt tacaaatcct
     7501 taacgtaagt cgcagaaaaa gccctttact tatcttaagt aaggctaaac tatttcctga
     7561 ctgtactaac ggttgaattg ttaaaaaatg ctacatatcc ttctgtttac ttaggataat
     7621 tttataaaaa ataaatctcg acaattggat tcaccacgtt tattagttgt atgatgcaac
     7681 tagttggatt attaaaataa tgtgacgaaa gctagcattt agatacgatg atttcatcaa
     7741 actgttaacg tgctacaatt gaacttgata tatgtcaacg aagcgtagtt ttattgggtg
     7801 tccggcccct cttagcctgt tatgttgctg ttaaaatggt taggatgaca gccgtttttg
     7861 acactgtcgg gtcctgaggg aaagtaccca cgaccaagct aatgatgttg ttgacgttga
     7921 tggaaagtgc atcaagaacg caattacgta ctttagtcat gttacgccga tcatgttaat
     7981 ttgcagcatg catcaggcag gtcagggact tttgtacttc ctgtttcgat ttagttggca
     8041 atttaggtag caaacatgca gaccccgcac attcttatcg ttgaagacga gttggtaaca
     8101 cgcaacacgt tgaaaagtat tttcgaagcg gaaggctatg atgttttcga agcgacagat
     8161 ggcgcggaaa tgcatcagat cctctctgaa tatgacatca acctggtgat catggatatc
     8221 aatctgccgg gtaagaacgg tcttctgtta gcgcgtgaac tgcgcgagca ggcgaatgtt
     8281 gcgttgatgt tcctgactgg ccgtgacaac gaagtcgata aaattctcgg cctcgaaatc
     8341 ggtgcagatg actacatcac caaaccgttc aacccgcgtg aactgacgat tcgtgcacgc
     8401 aacctgctgt cccgtaccat gaatctgggt actgtcagcg aagaacgtcg tagcgttgaa
     8461 agctacaagt tcaatggttg ggaactggac atcaacagcc gttcgttgat cggccctgat
     8521 ggcgagcagt acaagctgcc gcgcagcgag ttccgcgcca tgcttcactt ctgtgaaaac
     8581 ccaggcaaaa ttcagtctcg tgctgaactg ctgaagaaaa tgaccggccg tgagctgaaa
     8641 ccacacgacc gtactgtaga cgtgacgatc cgccgtattc gtaaacattt cgaatctacg
     8701 ccggatacgc cggaaatcat cgccaccatc cacggtgaag gttatcgctt ctgtggtgat
     8761 ctggaagatt aatcggcttt accaccgtca aaaaaaacgg cgctttttag cgccgttttt
     8821 atttttcaac cttatttcca gatacgtaat tgatcgtcca ttgtaacttc tttactggct
     8881 ttcattttcg gcagtgaaaa cgcataccag tcgatattac gagtcacaaa catcatgccg
     8941 gccagcgcca ccaccagcac actggttccc aacaacagcg cgctatcggc agagttgagc
     9001 agtccccaca tcacaccatc cagcaacaac agcgcgaggg taaacaacat gctgttgcgc
     9061 cagcctttca ataccgcctg caaataaata ccgttcatta acgccccaat cagactggcg
     9121 attatccatg ccacggtaaa accgatatgt tcagaaagcg ccagcaagag caaataaaac
     9181 atcaccaatg aaagccccac cagcaaatat tgcattgggt gtaaacgttg cgcggtgagc
     9241 gtttcaaaaa caaagaacgc cataaaagtc agtgcaatca gcagaatgac gtacttagtc
     9301 gcccagtccg ttaattggta ttgatcggct ggcgtcgtca ctgcgacgct aaacgcgggg
     9361 aagttttccc agccggtatc attgcctgaa gcaaaacgct caccgagatt attagcaaac
     9421 cagctgcttt gccattgcgc ctgaaaacct gactcgctaa cttcccgttt ggctggtaga
     9481 aaatcaccta aaaaactggg atgcggccag ttgctggtta agggcatttc gctattacgc
     9541 ccggcaggca ccacagaaag atcgccggta ccgcttaaat tcagtgccat attcagctgc
     9601 aggttctgct tccgccagtc cccttcgggt aaagggatat gcacgccctg cccgccttgc
     9661 tctaacccgg taccgggttc aatggtcagt gccgttccgt taacttcagg cgctttcacc
     9721 acaccaatac cacgcgcatc cccgacgcta atcacaataa agggcttgcc taagatgata
     9781 tttggcgcgt tgagctcgct aagacgcgaa acatcgaaat cggcttttag cgttaaatca
     9841 ctgtgccaga cctgaccggt ataaatccct atcttgcgtt cttccacgtt ctgattgcca
     9901 tcaaccatca atgactcagg taaccaaaaa tggataaaac ttcgtttccg ctccacggtt
     9961 ttatcctctt cctgcaccgt ataaagctcg gtcacaggaa tagcgataag cggcccaacg
    10021 agtttttgcg gcccgctggt actttggcga atcgcatctt ccacatcgct acggtaatcg
    10081 gcacgttcga caatcacttg ccgaatcagc attatcggaa tcaacaacag caatactgca
    10141 ccaaacaagg tggtcatttt ccagaacagg ggggatttca acatagtctt ctcctttgca
    10201 atggcagcag gatacgaagg ctatgtggga agaatttgaa gctatgtgaa gtgacggtga
    10261 agtcgaagcg aggccagcac gccaccttcc tgcacgttgc gcagcgtgac ttcgccgtta
    10321 aacaaacggg cgacctcact gacgaacgcc aaccccagac cgctgctttt ttgcccattt
    10381 gcacgcggca aagagtaaaa gcgttcaaaa atacgtgaca gcgcgtagtc aggaatacca
    10441 ctaccggtat ccagcacctt aagggcgacg tgttcctgat ccacttcggc gcttagcgtt
    10501 atgcgaccgc tctcgggggt aaaatcgatg gcgttatcca gtaaattccc cagcgcctgc
    10561 tccagtaacg ccggttcagc agcaacatta acctcagtgg gcataacatg tagagtgatg
    10621 tttttttctg ccaactgcac ggtgcgcgct tcgctgacgc ggcgaaataa tgccgccaca
    10681 tcaacaacag tcagaacgac ttcctgacga ttctccagtc ttgcctggcg tagtaacgtt
    10741 tctaccaatg cctgcatacg cgcattttgc gtcagaatgt tgtcggtaaa acgagccacc
    10801 acttccggtg gcggaccttc gcgtaaaatt tccgccgcgc cacgaatcgc cgccagtggg
    10861 ctttttagct catgggttaa cgcataaaca tactgctcaa tatagttttt cccttccagc
    10921 ttcacgcgca tactttccag cgcctgcgca agcttacgca actcgctact gccgagttca
    10981 ggaagaggaa cgggcttatt gtcagtgacg gaatcggcat agcgagtgag ccgggcaatg
    11041 gagcggttga tccaccaaac catgcctgcg ccaatcacca gtgcaatccc cagcaaaatg
    11101 gcgctggccc ataaaattcg ccgctcgcta cgcttaatga ccggagccat cgccgcgttc
    11161 ggtttgccta cgctcaaaac gccaataagc cgcgagccgt ccataatcgg cgcggcaaca
    11221 tacatcaccg aactttcggg atcggcagga ttttgcaacg tgctgcgcgc accatactga
    11281 ccacgcaacg ttagccagac gtcattccag cgcgaataat cctgcccaac ggctttattt
    11341 gccgaatcga acaatacttt accctgcgaa tcggtcatat agacatgata ttcattgcgc
    11401 actttgttaa tgccaccgat attggcgcga aacggacgat gttgtagctg attaaacgcc
    11461 tgcgccagtt gcccatgcgt tgggtcccca gagagcaaat cgggacgcgc cagctccgcc
    11521 agcaacgttg cggtgtcgat caacgtcccc tccgttgctc ttcgcacgcc cggtttaact
    11581 tctttgacaa aaatagccag tacgaaccag gccgccaccg ccaccagtaa aaaatagccc
    11641 agcagcaacc gcatgccgat acgcattaca ggcccctcag gctatatccc atgccgcgat
    11701 gagtattaat cggtgaaaga tcggggttga tggcacgcag cttggcatgc aacgttttaa
    11761 tgtgggtatc aacggtgcga tcgtaggtgt cctgcgcatc ttcccacacg ctatccatca
    11821 gttgctggcg ggaccagacg cggcctggcg acttgagtaa cgtcttcagc aataaaaact
    11881 cataccgcgt cagcgttaat ggcgtgtcaa accagctgat ctgcgccgcg ggttcattca
    11941 attcaaaatg accaatacgg atgacgggag acggcgacga gaacttcttc acccgacgca
    12001 gtaaggtgcg caccctggcg cacacttcgc ggggtgaaaa cggtttagcc acatagtcgt
    12061 cagcgccaat ttccagccca agcaggcgat cgacctcttc acttcgggcc gtcaggaaca
    12121 gtacaggtaa cgccggatgg agcgccagta actggctgca caattcaaag ccgctgatat
    12181 caggcaaacc aacgtcgaga atcatgacgt cgggaaccag ctggcgagct ttatccagca
    12241 ccggcaagcc tcgctcaaag acctcgacgg caaaaccttc ctgctgcaac atgtagacca
    12301 gcgtgtcggc tatcccttgc tcatcttcca ctaaccagac cgtttcccgt tgtataaatc
    12361 gcctctgtta ttgccgccac ggcatgacag gaaccgcgct aatcgcgttt ttcggcgaac
    12421 cttctacaac tttgtcggag taagccagat aagcgagcgc gttgcgtttg gcgtcataaa
    12481 agcgcacgac ctgtaacgac ttaaagacca gggacgtgcg ttttttgaat actacctcgc
    12541 cctgagcttt gccgttttta atacgatccg acagttcaat cggcccgact tgctgacaag
    12601 aaatagccgc atcggaggta tcttccgcca gacccaatcc ccctttaata ccacctgttt
    12661 tcgcccggct cacataacag gtgacatttt tcacatcggg atcgtcaaag gcttccacaa
    12721 caattttgtg atccgggccg atcattttaa atacggtgtc gaccgaacca atctcttctg
    12781 catgagccaa tggccccagc attatcaggc ttaaagacag gatcaaatgc ttgtatttca
    12841 tattgttacc atttttatct attacacgag caattagttc gtcacggtaa ataacatatt
    12901 gattaagaac aaaatctcaa ttcttttatt tccgtcaagc cctaaaacat actctactaa
    12961 gaaaaaaaca ctgaatgcta aaacagcaaa aaatgctatt atccaattac ctgatgtcag
    13021 gtgctcgttg ttgaaaggat gaggatattt tatggatcag gccggcatta ttcgcgacct
    13081 tttaatctgg ctggaaggtc atctggatca gcccctgtcg ctcgacaatg tagcggcgaa
    13141 agcaggttat tccaagtggc acttacagag aatgtttaaa gatgtcactg gccatgctat
    13201 tggcgcgtat attcgtgctc gtcgtttgtc gaaatcggcg gtcgcactac gcctgactgc
    13261 gcgtccgatt ctggacatcg cgctgcaata ccgcttcgac tctcaacaga catttacccg
    13321 cgcattcaag aagcagtttg cccagactcc tgcactttac cgccgttctc ctgaatggag
    13381 cgcctttggt attcgcccgc cgctacgtct gggtgaattc acaatgccag agcacaaatt
    13441 tgtcaccctg gaagatacgc cgctgattgg tgttacccag agctactcct gttcgctgga
    13501 gcaaatctct gatttccgcc atgaaatgcg ttatcagttc tggcacgatt ttctcggcaa
    13561 cgcgccgacc attccgccag tgctctacgg cctgaatgaa acgcgtccga gtcaggataa
    13621 agacgacgaa caagaggtat tctataccac cgcgttagcc caggatcagg cagatggcta
    13681 tgtactgacg gggcatccgg tgatgctgca gggcggcgaa tatgtgatgt ttacctatga
    13741 aggtctggga accggcgtgc aggagtttat cctgacggta tacggaacgt gcatgccaat
    13801 gctcaacctg acgcgccgta aaggtcagga tattgagcga tactacccgg cagaagacgc
    13861 caaagcggga gatcgcccaa ttaatctgcg ctgtgaactg ctgattccta tccgccgtta
    13921 acgctgcagc tcatctaatg caggggcgtc cagatgcgag atgtcccctg cggtttcaac
    13981 gacccagcca gacgccagcc acaggctttc ctgataatcc acgcgcgaaa tagaacagtt
    14041 acgcagacgt aagcggcgcg ctgcccatgc tggtaatccg agaatcgtac tcaccagaca
    14101 tcccagtgca ataccatggc tcaccagcag cggtcggctt ccctgcggta agtcccggca
    14161 agattccagt gccgcattga cgcgatcgct gagctcctgc attgactcgc cttcaggaat
    14221 acgcccgtca acggtgccat tgaccagctg ccgacgccag ttctcttctt cttcggtcag
    14281 agaatcgata tgtctttttt ccagcacacc catgtttaat tcacgcaggc gagaatcaaa
    14341 gatgatgtca cagccgcagg cctgggcgat gatttccgcc gtacgccggg tgcgtcctaa
    14401 atcgctactg ataatatgcg taatgccaag ctctttggca cgggttgcca cctgcatcgc
    14461 ctgttgctca cctttggcgg tcagcgggct gtcagactgg ccctgaatac gtcgctcggc
    14521 gttccactgc gtttcaccgt ggcggactag gtatacctgt aacatgcttt ttttccgtat
    14581 actgctgtaa cgttaatttt ctgagtaatg ctgattatgc accaagttgt ctgtgcgacc
    14641 accaatcccg ctaaaattca ggccattctg caggcatttc acgagatctt cggcgaagga
    14701 tcctgccata ttgcatccgt tgccgtcgag agcggtgtac cggaacagcc ctttggcagt
    14761 gaggaaacgc gcgctggcgc acgaaatcgg gtagcaaatg cccgccgttt acttccagag
    14821 gctgattttt gggtggcgat tgaagctggc atcgatggtg acagcacttt cagttgggtg
    14881 gtgattgaaa acaccagcca gcgcggcgag gcgcgttcgg ctactttgcc gttacctgcg
    14941 gtgattctgc agaaagtgcg tgaaggtgag gcgcttggtc ccgtgatgtc gcgttatacc
    15001 ggtattgatg agattggtcg taaggaaggg gcgatcggcg tgtttaccgc cgggaaactc
    15061 actcgcgcca gcgtctatca ccaggcggtg atcctggcgc tgagtccgtt tcacaatgcc
    15121 gtgtatcagg cattgtagga cggataaggc gttcacgccg catccggcat tcggtgcacg
    15181 atgcctgatg cgacgctgcc gcgtcttatc aggcctacaa aatcaatcgc ttttcagcaa
    15241 cacctcttcc agccactggc gcagctccac gggcgcggct ttcaggctgt tagatccacg
    15301 cgtaatcgtc gcgatgcccg cgccgagttc attttttaac tcacgctgac tcatttcgcc
    15361 acgcaacagc tcttcgacaa tacgcacgcg agtccccaac gcttcgcgct catctggcgt
    15421 cagcatcagg tttaacaacg gtaaatggag atcgttttgg taggcattct taagcaggtc
    15481 gacaaaacgt aaccactcct ggtgacgctg ttctgccatc gctgctgaat agggtgattg
    15541 ttgggccata atatgtcgcc attgttagcg ggggaagcaa aatgcctccc ccggttgtac
    15601 tcgctaaaga gtacgatagc atatcataaa cgtgcggatc agtaacgacg tccccattcc
    15661 gtggcgctca tcaacgtcgg tttatccccc atgaaataac ggtagtaagc gtcataagcc
    15721 agcacgttct tcacataacc gcgcgtctcg gagaacggaa tactctcgac aaatgccaca
    15781 gcgtcgatac gcccggcgct gttgccaagc caggttcgca cccgtccagg tccggcgtta
    15841 taagctgctg aggagaaaat acggttattg ccaaactgct gataaacata ttgcaggtaa
    15901 ctggtgccaa tgttgatatt cgtttccgga tccagcaatt gcccagggct gctataacca
    15961 ggaatagaga acatcttcac cgtatgggtc gctgtaccag gcataatctg catcaggccg
    16021 ctggccccta ccggtgattt cactttcgga ttccaggcgc tctcctgacg ggcaatcgcc
    16081 atcgcatagc tttgcgggat ctctttccca ctggtgtaac gtttgaaaag atcgttgtaa
    16141 gccagcggga atcgctcttc cagatgatcc caaagcttcc cggcgatcgt tgcctgaacg
    16201 ctaagatccc accattggtt attgaaagca taccgagcca gttgagcctg ctctgttttt
    16261 gacttgctct tcaccagatt ggcccactcg ctacgcgcgg tgttatcgag attccagtac
    16321 atcaactcgc gcacgcgcgc catctccggc ccctgagtca gggcgctgtc aacattctgc
    16381 ggcgctttat caatcttcag ctcatactct tctccgatgc gttgtgctgc aaccatcggg
    16441 tagaaaccac gctgttgcat gagttgatgc aaaatctctt tcgcttcagc ttcacgtccg
    16501 cgttccagca ataaatccgc ctgccagtaa cgccattcat ctttctcttt tgcttccatc
    16561 ggcagacgcg ccagccaggt attcaggccg cgacgatcgc cggtgccaag tgccattcgc
    16621 acacggcgtt caatcaacga cgtggattgc gagcgcataa tggcgtcatc gcgccatttc
    16681 gcctgctcgt cggtgacatc gttgcccatc aaacgccagg cgacgatatc gcgcagctcc
    16741 tgaatctgat cttcattaag ctgctgcgcc tgggcaagcg acgggatcat cagccgtgca
    16801 ttttcagcat cctgccgcgc cacactggca aacgccaccg ctgccatttg acgggtaaaa
    16861 tcggtcgctc cggttgtacg cgcgaaggtc agtaccgtat tagggttgtt cgccagtgaa
    16921 atgattgccg aggcgatagt ctggtaatcg gcaggcatct gccctgccag cactgttacc
    16981 aggccagtgt tacccgcttt catcgccaga cggatacgct ctaaatacgc cagcggatct
    17041 tgtttacctg acgcacgcca gacgctaaat aacttgtcac aggcgttagg ctggctcttg
    17101 ccggttagcc acagctcttt cgccccttgc caggcttctt cactctgccc ggtgttccat
    17161 ttcgcatagt agtaattaca ttgcgcttcg gtagttccgg gcttttccgg gctaaaggct
    17221 aacaagccac gccagtcttc acgccgcgcc agttcattga cgaaacgaga ttgcagcgtg
    17281 cgagcgggag gaagcgtggg gttagcgcga acaaagttag tgaccgtcac cgccggttga
    17341 ttcatcagat catcggtgat ctggcggtat tccaggtagg gataaagcgg ataatccttc
    17401 agtccaggca tcatttgttc gaccacatcc atttgtcgat tatcccaggc ctgcttgatt
    17461 tgcgcgtaac ggctacgctg ctcatccagt gagtcggctc gcgccacgct gctgaccgtc
    17521 agcagacaga caccggcagc caacagccgc caggtaactt gtttggcttt ttccacaagc
    17581 gcatcctcta agtgtaaata catcaatgca gcgtcatgcc gcgtaataga tttatgctaa
    17641 ccagtcattg ccgtttacgc cacgttacgg acactttttt acttttactg cgaaggtgat
    17701 cgaccaactg ggattaggcg gggaaaaagg ctacacttcg cctttgaaaa ctcatcatca
    17761 cgataaacat agaggcgaag tccaacgtgg ctcaattcgt ttataccatg catcgtgtcg
    17821 gcaaagttgt tccgccgaaa cgtcatattt tgaaaaacat ctctctgagt ttcttccctg
    17881 gggcaaaaat tggtgtcctg ggtctgaacg gcgcgggtaa gtctaccctg ctgcgcatta
    17941 tggcaggcat tgataaagac atcgaaggtg aagcgcgtcc gcagccagac atcaagattg
    18001 gttacctgcc gcaggaaccg cagctgaacc cggaacacac cgtgcgtgag tccattgaag
    18061 aagcggtttc tgaagtggtt aacgccctga aacgcctgga tgaagtgtat gcgctgtacg
    18121 ccgatccgga tgccgatttt gacaagctgg ccgctgaaca aggccgtctg gaagagatca
    18181 ttcaggctca cgacggtcat aacctgaacg tacagctgga gcgtgcggcg gatgcgctac
    18241 gtctgccgga ctgggacgcg aaaatcgcta acctctccgg tggtgagcgt cgtcgcgtag
    18301 cgttgtgccg cctgctgctg gaaaaaccag acatgctgct gctcgacgaa ccgaccaacc
    18361 acctggatgc cgaatccgtg gcctggctgg aacgcttcct gcacgacttc gagggcaccg
    18421 tggtggcgat tacccacgac cgttacttcc tcgataacgt tgcaggctgg atcctcgaac
    18481 ttgaccgcgg tgaaggtatt ccgtgggaag gcaactactc ctcctggctg gagcagaaag
    18541 atcagcgcct ggcgcaggaa gcttcacaag aagcggcgcg tcgtaagtcg atcgagaaag
    18601 agctggagtg ggtacgtcag ggaactaaag gccgccagtc gaaaggtaaa gcacgtctgg
    18661 cacgctttga agagctgaac agcaccgaat atcagaaacg taacgaaacc aacgaactgt
    18721 ttattccacc tggaccgcgt ctgggcgata aagtgctgga agtcagcaac ctgcgtaaat
    18781 cctacggtga tcgcctgctg attgatgacc tgagcttctc gatcccgaaa ggggcaatcg
    18841 tcgggatcat cggtccgaac ggcgcgggta aatcgaccct gttccgtatg atctctggtc
    18901 aggaacagcc ggacagcggc accatcactt taggtgaaac ggtgaaactg gcatcggttg
    18961 atcagttccg tgactcaatg gataacagca aaaccgtttg ggaagaagtt tccggcgggc
    19021 tggatattat gaagatcggc aacaccgaga tgccaagccg cgcctacgtt ggccgcttta
    19081 actttaaagg ggttgatcag ggtaaacgcg ttggtgaact ttccggtggt gagcgcggtc
    19141 gtctgcatct ggcgaagctg ctgcaggttg gcggcaacat gctgctgctc gacgaaccaa
    19201 ccaacgacct ggatatcgaa accctgcgcg cgctggaaaa cgccctgctg gagttcccgg
    19261 gctgtgcgat ggttatctcg cacgaccgtt ggttcctcga ccgtatcgcc acgcacatcc
    19321 tggactacca ggatgaaggt aaagttgagt tcttcgaagg taactttact gagtacgaag
    19381 agtacaagaa acgcacgctg ggcgcagacg cactggagcc gaagcgtatc aagtacaagc
    19441 gtattgcgaa gtaatgcgta aaatgccgga tgcggcgtga acgccttatc cggcctacaa
    19501 accaagaaat ttcaatacgt tgtgaatctc caggcctgat aagacgcggt aagcgtcgca
    19561 tcaggcattg agcaccgaat gccggatacg gcgtgaacgc cttttccggc ctacaatagc
    19621 cttagtatcc gcaatttcgt agcgggtcac ccgtgctgat tcttttaaac cttgtttaat
    19681 ctcgacagcc tcttccagag aggttttcaa ctcatcaaaa aacttatttt atacctcgcc
    19741 tttgagccgt ttcatcgcgg ttatctctgc tcccccatca tctcccgcac cagctccacg
    19801 cagcgcagga aacggctgtc ataatcgtcc tcttcaaccc gcacgaattc gatattgttc
    19861 tcttccagca tctccaccag caagttctgg aactctttgc gatccaccga actgccgagg
    19921 ctgcgtaaac catccgccac ccacggcgtg ttgttctcca gcaggatcac cagatcgaaa
    19981 cggtattcat caatcaacgc ctgtacgaac ggatgctcac gcccttcgta ctttttgcag
    20041 aacgcctggg tagtgacaaa atcagtgtca ataaacgcca ctttattggc atatttcact
    20101 gcaaaatcaa tgtattgtgc gtggcccagc gcgattttat cgtaatcgga atactgcaat
    20161 gcgatctcat caccgccgag gtgtgaaaag acataatcgc gaccatattc ccacgcactg
    20221 gtggtattga agatattggc aagtttgttt accagggtgg atttaccgct cgactcgcca
    20281 ccaaggatcg ccacggtacg tacaaagaac ggcttcactt ccgtaggaat atattcccag
    20341 tagcggaacg ggttttcgcg gatctgcgca ccgctgatac tcataaaggt acgtttcgga
    20401 tcgaccagca ccgtatcgat ccccagatgt tccatatact gtggcgcatc ggcttcttcc
    20461 gaggtgtaga tcaggtccgg ctgaatcccc ttttcagcca taaacttttt gatgccgttg
    20521 ctccacacat cccagccgtg cggatacggc tccatgccct cctcgttgaa agcatgaatg
    20581 cgaatatttt tctgatattt aaaagtttgt aataaccagc gcagacgatc cggcaccgtg
    20641 ggctgctgcg acatggcgct gtcttcaaac aacgcgcggt cgcgggtatc gtcaaaaccc
    20701 ataatgatat gcagctcgtc aacctggcta caggcgcgct ggataaggta gatatgtccg
    20761 gtatgcagcg ggtagaactt accgaatacg acaccaatgg ttttcttctg tcggggaaat
    20821 tcaagcccca aaaaacggtg caacgcctcc agcttttgcg cgctggggct tttaattttg
    20881 gcattcagta actggcttaa ataccctttg gtcataccgc tggcatcagc tacctgctgt
    20941 agcgtgcagc cctgttgctt gatggcagtt ttcaggtaat caaatgacga catatctccc
    21001 tccgtatctc tcattataag tcgtcgaaca cgctaagcgc gtcggagagt tttttaacgc
    21061 caaaaatctg catcccttcc ggcgcttttt tcggtacgtt agccgccgga acaatcgccc
    21121 ggcgaaaacc gtgtttcgcc gcttctgaaa ttcgttcctg accgctgggc accgggcgga
    21181 tctcccctgc cagcccgact tcaccaaaca ccaccagatc ctgtggcagc ggtctgtcac
    21241 gcaggctgga aaccatcgcc agcagtaacg ctaaatcggc actggtttcg gttaccttca
    21301 cgccgccgac cacgttcaca aatacatcct gatcggccat ttgcagacca ccgtgacggt
    21361 gcaagacagc cagcaggatt gccagacggt tttgttccag ccccactgcc acgcggcgcg
    21421 gattcgccat catcgagtga tcgaccagcg cctgaatctc caccagcaac ggacgcgttc
    21481 cttcccacac caccatcacg gagctgccgg aggtcacttc atctccgcga cttaagaaaa
    21541 ttgccgaagg gttgctgact tcacgcagcc cctgttcggt catcgcgaag acgcccagct
    21601 cattcaccgc gccgaagcgg tttttatggc tgcgcaaggt gcgaaaacgg gagtcggcat
    21661 caccatccag aagcaccgaa cagtcgatac agtgttccag cactttaggg ccagccagcg
    21721 agccatcttt ggttacgtgc ccaaccatga caatcgccac accgcgcgtt ttggcgaagc
    21781 gcgtcagata agccgccgtt tcacgcacct gcgccacgct accaggcgat gattgtacat
    21841 ccgccatatg catcacctgg atcgagtcaa ttaccatcag tttcggttgc tcttcttcgg
    21901 caatcaggca gatctgttcg atgctggttt ccgacaacat attgagattg tcagtcggca
    21961 ggccaaggcg atgagcgcgc attgccacct gttgcagcga ctcttcgcca gtgacataca
    22021 gcgttttcat ctgctgggcc agtttgcaca gcgtttgcag tagcagcgtg gatttccccg
    22081 cacctgggtt accgccaatc agaatggcgc ttcccggcac cacgccgccg cctagtacgc
    22141 ggtcgaactc tttaaatccg gtggaaaaac gcggcagctc ttcaaggctg atatcggaga
    22201 gtttctggac ttttgccacc ccggcgctac cggcatagcc gctgagacgc tcgttacgcg
    22261 ccaccgttgg cgacgcagca agacgcacct cggtgatggt gttccaggca tgacaggcac
    22321 tgcactgccc ctgccagcgc ggataatcgg ccccgcattc attacaaaca aaggcgcgtt
    22381 ttggagcttt tgccacggtg tacctcgtta atgctgtgcc gcccgcagga tggcgggcga
    22441 gcaattactt ctgattcagg ctgccagaga ggatgcagaa tacccccatc aggtcagcgt
    22501 gacggatggt gacttccgcc ttttcattca cttttggctt ggcatggtag gcaatcccca
    22561 gccctgctgc tttgatcatc ggcaggtcat tagctccatc gccaatcgcc acggtctgcg
    22621 ccagcgggat ttcatactcc tgcgcgaggc gagtcaaagt tttcgctttg tactgcgcgt
    22681 ctacgatgtc gccgatcaca ttgccggtaa atttaccgtc catgatctcc agttcattgg
    22741 ctaccacggc ggtcaggcgc agcttgtcgc gcagatattc agcaaagaaa gtaaagccgc
    22801 cggaggcaat cgccactttc cagcccagcg tttccagctt gagcaccagt tgcgttaagc
    22861 ctggcatcag cggcagattt tcacgcacct gttgcagaat attggcgtca gcgcctttca
    22921 gcgtcgccac acggctgcgc aggctggcgg taaaatcgag ttcgccgcgc atcgcccgtt
    22981 cggttacttc cgccaccatc tcgcccgttc cggccagttt ggcaatttca tcaatacatt
    23041 caatctggat ggcggtggaa tccatgtcca tcaccagcaa acccggcgtg cgcaggtgcg
    23101 ggattttccc cagcggggcg acatccagtt gcgcttcatg ggccaggcgc gtagcccgtg
    23161 cggtgagtga acctgccaga cgaatcacct gataatcttc cacgcaccag gcggcaacaa
    23221 tcaccatcgc cgcacccagt ttgctctggt attgggtcag acgctgttta tccagcccac
    23281 gaccatacag cagccagccg ctacgacctg cgtggtaatc cagtggcatc acttcatcac
    23341 cacttaatga aagaggcaga ccaggccata aagagacatc ttcaggcagg tcgcaccagg
    23401 taatgttagg cattaaggat cctgtaaaat cgttcgaagc agggaaaata acgcatgagg
    23461 ctaccttgta tccattgctt ctggcaacat taagtctcaa attttcaaag ggtggaagat
    23521 ggctcgcaca aaactgaaat tccggctgca tcgggcagtg attgtcctgt tctgtcttgc
    23581 cttgttagtg gcgctgatgc agggagcgtc atggtttagt caaaaccacc agcgacaacg
    23641 taatccacag ctggaagaac tggcccgcac cctggcgcgt caggtaacgc tgaacgttgc
    23701 accgctgatg cgtaccgact caccggatga aaaacgcatt caggcgatcc tcgatcagtt
    23761 aacggatgaa agccgtatcc tcgacgcggg tgtgtatgac gaacaaggcg atcttatcgc
    23821 acgttctggc gaaagcgtcg aagtgcgcga ccggctggcg ctcgacggta aaaaagcggg
    23881 cggctatttt aaccagcaga ttgtcgaacc aattgccggg aaaaacggac cgctcggcta
    23941 tctgcgcctg acgctcgaca cccatacgct cgccaccgaa gcccaacagg tggataacac
    24001 cactaacatt ttacgcctga tgttgctgct ttcactggca atcggcgtag tgctgacccg
    24061 cacgctgcta cagggtaaac gtacccgctg gcagcaatcg cccttcctgt taaccgccag
    24121 caaaccggtg ccggaagagg aagaaagcga gaaaaaagag tgacccatta ctacaagaaa
    24181 ggaaatcgtt atgtccacat tacgcctgct catctctgac tcttacgacc cgtggtttaa
    24241 cctggcggtg gaggagtgta tttttcgcca gatgcccgcc acgcagcgcg ttctgtttct
    24301 ctggcgcaat gccgacacgg tagtgattgg tcgcgcgcag aacccgtgga aagagtgtaa
    24361 tacccggcgg atggaagaag ataacgtccg cctggcacgg cgcagtagcg gtggcggcgc
    24421 ggtgttccac gatctcggca atacctgctt tacctttatg gctggcaagc cggagtacga
    24481 taaaaccatc tccacgtcga ttgtgctcaa tgcgctgaac gcgcttggcg tcagcgccga
    24541 agcgtccggg cgtaacgatc tggtggtgaa aaccgccgaa ggcgaccgca aagtctcagg
    24601 atcggcctat cgcgaaacca aagatcgtgg cttccaccac ggcaccttgc tgctcaatgc
    24661 cgaccttagc cgcctggcaa actatctcaa tccggataaa aagaaactgg cggcgaaagg
    24721 cattacctca gtgcgttccc gcgtgaccaa cctcaccgag ctgctgccgg ggatcaccca
    24781 tgagcaggtt tgcgaggcca taaccaaggc ctttttcgcc cattatggcg agcgtgtaga
    24841 agcggaaatc atctccccgg acaaaacgcc agacttgcca aacttcgccg aaatctttgc
    24901 ccgtcagagt agctgggaat ggaacttcgg tcaggctccg gcattctcgc atctgctgga
    24961 tgaacgcttt agctggggcg gcgtggaact gcatttcgac gttgaaaaag gccatatcac
    25021 ccgcgcccag gtgtttaccg acagcctcaa ccccgcgccg ctggaagccc tcgccgggcg
    25081 actgcaaggc tgcctgtacc gcgcggatat gctgcaacaa gagtgcgaag cgctgttggt
    25141 tgacttcccg gaccaggaaa aagagctacg ggagttgtcg acgtggatag cgggggcggt
    25201 aaggtaactc gctgattaaa ataccaaagg aagggaatat gatcgatctg gaaaaccagg
    25261 agcgggagat tatcaattta atgttaagcc agcgtatttc ctggctagcc gccgtgcgta
    25321 tcaggcataa attatcgctt gctgaagtaa gtaaaatgtt aggtatctcg attaattctc
    25381 taaaatagat cgaaaagacg gaacggttta gctcaaacat caaaagcaaa atggcagaaa
    25441 tttatggttg tccaccagag ttgctgatat gcccttcctg gatgacggct gaacataaat
    25501 gaatagatt
//
LOCUS       DADLFA010000041        25135 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000041 DADLFA010000000
VERSION     DADLFA010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25135
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.041"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..404)
                     /locus_tag="JQH65_001429"
     CDS             complement(<1..404)
                     /locus_tag="JQH65_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAY6138897.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGTIVGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLP"
     gene            783..1094
                     /gene="rpsJ"
                     /locus_tag="JQH65_001430"
     CDS             783..1094
                     /gene="rpsJ"
                     /locus_tag="JQH65_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAY6138898.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            1127..1756
                     /gene="rplC"
                     /locus_tag="JQH65_001431"
     CDS             1127..1756
                     /gene="rplC"
                     /locus_tag="JQH65_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAY6138899.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            1767..2372
                     /gene="rplD"
                     /locus_tag="JQH65_001432"
     CDS             1767..2372
                     /gene="rplD"
                     /locus_tag="JQH65_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAY6138900.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            2369..2671
                     /gene="rplW"
                     /locus_tag="JQH65_001433"
     CDS             2369..2671
                     /gene="rplW"
                     /locus_tag="JQH65_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAY6138901.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            2689..3510
                     /gene="rplB"
                     /locus_tag="JQH65_001434"
     CDS             2689..3510
                     /gene="rplB"
                     /locus_tag="JQH65_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAY6138902.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            3527..3805
                     /gene="rpsS"
                     /locus_tag="JQH65_001435"
     CDS             3527..3805
                     /gene="rpsS"
                     /locus_tag="JQH65_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAY6138903.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            3820..4152
                     /gene="rplV"
                     /locus_tag="JQH65_001436"
     CDS             3820..4152
                     /gene="rplV"
                     /locus_tag="JQH65_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAY6138904.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            4170..4871
                     /gene="rpsC"
                     /locus_tag="JQH65_001437"
     CDS             4170..4871
                     /gene="rpsC"
                     /locus_tag="JQH65_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAY6138905.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            4884..5294
                     /gene="rplP"
                     /locus_tag="JQH65_001438"
     CDS             4884..5294
                     /gene="rplP"
                     /locus_tag="JQH65_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAY6138906.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            5294..5485
                     /gene="rpmC"
                     /locus_tag="JQH65_001439"
     CDS             5294..5485
                     /gene="rpmC"
                     /locus_tag="JQH65_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAY6138907.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            5485..5739
                     /gene="rpsQ"
                     /locus_tag="JQH65_001440"
     CDS             5485..5739
                     /gene="rpsQ"
                     /locus_tag="JQH65_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAY6138908.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            5904..6275
                     /gene="rplN"
                     /locus_tag="JQH65_001441"
     CDS             5904..6275
                     /gene="rplN"
                     /locus_tag="JQH65_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013975775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAY6138909.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            6286..6600
                     /gene="rplX"
                     /locus_tag="JQH65_001442"
     CDS             6286..6600
                     /gene="rplX"
                     /locus_tag="JQH65_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAY6138910.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            6615..7154
                     /gene="rplE"
                     /locus_tag="JQH65_001443"
     CDS             6615..7154
                     /gene="rplE"
                     /locus_tag="JQH65_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAY6138911.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            7169..7474
                     /gene="rpsN"
                     /locus_tag="JQH65_001444"
     CDS             7169..7474
                     /gene="rpsN"
                     /locus_tag="JQH65_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAY6138912.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            7508..7900
                     /gene="rpsH"
                     /locus_tag="JQH65_001445"
     CDS             7508..7900
                     /gene="rpsH"
                     /locus_tag="JQH65_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAY6138913.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            7913..8446
                     /gene="rplF"
                     /locus_tag="JQH65_001446"
     CDS             7913..8446
                     /gene="rplF"
                     /locus_tag="JQH65_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAY6138914.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRIKEAKKK"
     gene            8456..8809
                     /gene="rplR"
                     /locus_tag="JQH65_001447"
     CDS             8456..8809
                     /gene="rplR"
                     /locus_tag="JQH65_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAY6138915.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            8824..9327
                     /gene="rpsE"
                     /locus_tag="JQH65_001448"
     CDS             8824..9327
                     /gene="rpsE"
                     /locus_tag="JQH65_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAY6138916.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            9331..9510
                     /gene="rpmD"
                     /locus_tag="JQH65_001449"
     CDS             9331..9510
                     /gene="rpmD"
                     /locus_tag="JQH65_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAY6138917.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            9514..9948
                     /gene="rplO"
                     /locus_tag="JQH65_001450"
     CDS             9514..9948
                     /gene="rplO"
                     /locus_tag="JQH65_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAY6138918.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            9956..11287
                     /gene="secY"
                     /locus_tag="JQH65_001451"
     CDS             9956..11287
                     /gene="secY"
                     /locus_tag="JQH65_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAY6138919.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            11319..11435
                     /gene="rpmJ"
                     /locus_tag="JQH65_001452"
     CDS             11319..11435
                     /gene="rpmJ"
                     /locus_tag="JQH65_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAY6138920.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            11582..11938
                     /gene="rpsM"
                     /locus_tag="JQH65_001453"
     CDS             11582..11938
                     /gene="rpsM"
                     /locus_tag="JQH65_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018356918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAY6138921.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            11955..12344
                     /gene="rpsK"
                     /locus_tag="JQH65_001454"
     CDS             11955..12344
                     /gene="rpsK"
                     /locus_tag="JQH65_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAY6138922.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            12378..12998
                     /gene="rpsD"
                     /locus_tag="JQH65_001455"
     CDS             12378..12998
                     /gene="rpsD"
                     /locus_tag="JQH65_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAY6138923.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            13024..14013
                     /gene="rpoA"
                     /locus_tag="JQH65_001456"
     CDS             13024..14013
                     /gene="rpoA"
                     /locus_tag="JQH65_001456"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAY6138924.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            14054..14437
                     /gene="rplQ"
                     /locus_tag="JQH65_001457"
     CDS             14054..14437
                     /gene="rplQ"
                     /locus_tag="JQH65_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAY6138925.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            14544..14912
                     /locus_tag="JQH65_001458"
     CDS             14544..14912
                     /locus_tag="JQH65_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HAY6138926.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILRGIRHDDPQYQEVSRRLSLLELKLRQAGF
                     STDFLRGDYADKLLDKINDN"
     gene            14923..15348
                     /gene="zntR"
                     /locus_tag="JQH65_001459"
     CDS             14923..15348
                     /gene="zntR"
                     /locus_tag="JQH65_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HAY6138927.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            15404..15622
                     /gene="arfA"
                     /locus_tag="JQH65_001460"
     CDS             15404..15622
                     /gene="arfA"
                     /locus_tag="JQH65_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome alternative rescue factor ArfA"
                     /protein_id="HAY6138928.1"
                     /translation="MSRYQHTKGQIRDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(15619..16029)
                     /gene="mscL"
                     /locus_tag="JQH65_001461"
     CDS             complement(15619..16029)
                     /gene="mscL"
                     /locus_tag="JQH65_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAY6138929.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(16159..17535)
                     /gene="trkA"
                     /locus_tag="JQH65_001462"
     CDS             complement(16159..17535)
                     /gene="trkA"
                     /locus_tag="JQH65_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HAY6138930.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(17557..18846)
                     /gene="rsmB"
                     /locus_tag="JQH65_001463"
     CDS             complement(17557..18846)
                     /gene="rsmB"
                     /locus_tag="JQH65_001463"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAY6138931.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAKFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(18892..19839)
                     /gene="fmt"
                     /locus_tag="JQH65_001464"
     CDS             complement(18892..19839)
                     /gene="fmt"
                     /locus_tag="JQH65_001464"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAY6138932.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERYIRAFNPWPMSWLEIERQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLA"
     gene            complement(19854..20363)
                     /gene="def"
                     /locus_tag="JQH65_001465"
     CDS             complement(19854..20363)
                     /gene="def"
                     /locus_tag="JQH65_001465"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAY6138933.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            20493..21617
                     /gene="dprA"
                     /locus_tag="JQH65_001466"
     CDS             20493..21617
                     /gene="dprA"
                     /locus_tag="JQH65_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAY6138934.1"
                     /translation="MVDTDIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     YALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLATSLLEHGGALVSEFPLDVSPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            21589..22062
                     /locus_tag="JQH65_001467"
     CDS             21589..22062
                     /locus_tag="JQH65_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HAY6138935.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLTEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLEI
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            22091..22633
                     /locus_tag="JQH65_001468"
     CDS             22091..22633
                     /locus_tag="JQH65_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6138936.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            22638..23210
                     /gene="tsaC"
                     /locus_tag="JQH65_001469"
     CDS             22638..23210
                     /gene="tsaC"
                     /locus_tag="JQH65_001469"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HAY6138937.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVLGETGGRLNPSEIRDALTGELFRQG"
     gene            23215..24033
                     /gene="aroE"
                     /locus_tag="JQH65_001470"
     CDS             23215..24033
                     /gene="aroE"
                     /locus_tag="JQH65_001470"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAY6138938.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLQGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHLGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            24030..24287
                     /locus_tag="JQH65_001471"
     CDS             24030..24287
                     /locus_tag="JQH65_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HAY6138939.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(24263..24817)
                     /locus_tag="JQH65_001472"
     CDS             complement(24263..24817)
                     /locus_tag="JQH65_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAY6138940.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGGVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6422 a         5973 c         6547 g         6193 t
ORIGIN      
        1 aaaggcagcg ggttttttaa tgattctttt cctctcatga gcagaccaat aactacggct
       61 ccgcaagcga atgaggcagc aaggaaaacc agtcgtggta agaaggccca tgaatgccag
      121 gcgccaaggg cggcgagaaa cttcacatca ccatagccta atccttcttt atgtcgcagt
      181 atgcgatacc cccagtaaat gacagcgaat gtgccgtaac caacaattgt cccccataac
      241 gcatcggcta aaccatcagg atgacaaact tgatagaaca aaagacctga ccagagtaac
      301 gggcacgtaa atctgtcagg taacaagcca tgctttgcat cccagaaaaa aagtagggct
      361 gataaacaag cgtacaaaat cagaaaggga agggtagtgt ccataaaaca tcctctgtaa
      421 ttggagtatc accatcgaat aactccggat gctcagcaac agagagagcg ctgatttgtt
      481 aacctgattc cggtgaaaaa cgctatcttc acaaccttac aaatttcgtg gaatgcagct
      541 cgcaatctgc gcgttgcgat taatttctac ccgatgcttg cgtcaggctc agatttgcgt
      601 ataatgcgcg ggcttgtcgt agttgacagc aggttcaatc tgaacctcag ttagccgaat
      661 ttggctacct aacaatgctc ccaatcgggg agctacgtaa gaacggttac actctcccat
      721 caatcgtaat gggtctgagg agtaatcatt ttcgtttata aaataattgg agctctggtc
      781 tcatgcagaa ccaaagaatc cgtatccgcc tgaaagcgtt tgatcatcgt ctgatcgatc
      841 aagcaaccgc ggaaatcgtc gagactgcca agcgcactgg tgcgcaggtc cgtggtccga
      901 tcccgctgcc gacacgcaaa gagcgcttca ctgttctgat ctccccgcac gtcaacaaag
      961 acgcgcgcga tcagtacgaa atccgtactc acttgcgtct ggttgacatc gttgagccaa
     1021 ccgagaaaac cgttgatgct ctgatgcgtc tggatctggc tgccggtgta gacgtgcaga
     1081 tcagcctggg ttaatcaggt cattgagcga ttgagaggtt gaaacaatga ttggtttagt
     1141 cggtaaaaaa gtgggtatga cccgtatctt cacagaagac ggcgtttcta tcccagtaac
     1201 cgtaatcgaa gttgaagcaa accgcgttac tcaggttaaa gacctggcta acgatggcta
     1261 ccgtgctatt caggtgacca ccggtgctaa aaaagctaac cgtgtgacca agcctgaagc
     1321 tggccacttc gctaaagctg gcgtagaagc tggccgtggt ctgtgggaat tccgcctggc
     1381 tgaaggcgaa gagttcactg taggtcagag cattagcgtt gaactgtttg ctgacgttaa
     1441 aaaagttgac gtaactggca cctctaaagg taaaggtttc gcaggtaccg ttaagcgctg
     1501 gaacttccgt acccaggacg ctactcacgg taactccttg tctcaccgcg ttccgggttc
     1561 tatcggtcag aaccagactc cgggcaaagt gttcaaaggc aagaaaatgg caggtcagat
     1621 gggtaacgaa cgtgtaaccg ttcagagcct tgacgtagta cgcgttgacg ctgagcgcaa
     1681 cctgctgctg gttaaaggtg ctgtcccggg tgcaaccggt agcgacctga tcgttaaacc
     1741 agctgtgaag gcgtaaggag atagcaatgg aattagtatt gaaagacgcg cagagcgcgc
     1801 tgactgtttc cgaaactacc ttcggtcgtg atttcaacga agcgctggtt caccaggttg
     1861 ttgttgctta tgcagctggt gctcgtcagg gtactcgtgc tcagaagact cgtgctgaag
     1921 taactggttc cggtaaaaaa ccgtggcgcc agaaaggcac cggccgtgcg cgttctggtt
     1981 ctatcaagag cccgatctgg cgttctggtg gcgtgacctt tgctgctcgt ccgcaggacc
     2041 acagtcaaaa agttaacaag aagatgtacc gcggcgcgct gaaaagcatc ctgtccgaac
     2101 tggtacgtca ggatcgtctg atcgttgtcg agaagttctc tgtagaagcg ccgaaaacta
     2161 agctgctggc acagaaactg aaagacatgg ctctggaaga tgtgctgatc atcaccggtg
     2221 agctggacga aaacctgttc ctggctgcgc gcaacctgca caaggttgac gtacgcgatg
     2281 caactggtat cgacccggtt agcctgatcg ccttcgacaa agtcgtaatg actgctgatg
     2341 ctgttaagca agttgaggag atgctggcat gattcgtgaa gaacgtctgc tgaaggtgct
     2401 gcgtgcaccg cacgtttctg aaaaagcgtc tactgcgatg gaaaaatcca acaccatcgt
     2461 actcaaagtt gctaaagacg cgaccaaagc agaaatcaaa gctgctgtgc agaaactgtt
     2521 tgaagtcgaa gtcgaagtcg ttaacaccct ggtagttaaa gggaaagtta aacgtcacgg
     2581 acagcgtatc ggtcgtcgta gcgactggaa aaaagcttac gtcaccctga aagaaggcca
     2641 gaatctggac ttcgttggcg gcgctgagta agtcggagga gtaatacaat ggcagttgtt
     2701 aaatgtaaac cgacatctcc gggtcgtcgc cacgtagtta aagtggttaa ccctgagctg
     2761 cacaagggca aaccttttgc tccgttgctg gaaaaaaaca gcaaatccgg tggtcgtaac
     2821 aacaatggcc gtatcaccac tcgtcatatc ggtggtggcc acaagcaggc ttaccgtatt
     2881 gttgacttca aacgcaacaa agacggtatc ccggcagttg ttgaacgtct tgagtacgat
     2941 ccgaaccgtt ccgcgaacat cgcgctggtt ctgtacaaag acggtgaacg ccgttacatc
     3001 ctggccccta aaggcctgaa agctggcgac cagattcagt ctggcgttga tgctgcaatc
     3061 aaaccaggta acaccctgcc gatgcgcaac atcccggttg gttctactgt tcataacgta
     3121 gaaatgaaac caggtaaagg cggtcagctg gcacgttccg ctggtactta cgttcagatc
     3181 gttgctcgtg atggtgctta tgtcaccctg cgtctgcgtt ctggtgaaat gcgtaaagta
     3241 gaagcagact gccgtgcaac tctgggcgaa gttggcaatg ctgagcatat gctgcgcgtt
     3301 ctgggtaaag caggtgctgc acgctggcgt ggtgttcgtc cgaccgttcg cggtaccgcg
     3361 atgaacccgg tagaccaccc acatggtggt ggtgaaggtc gtaactttgg taagcacccg
     3421 gtaactccgt ggggcgttca gaccaaaggt aagaagaccc gcagcaacaa gcgtactgat
     3481 aaattcatcg tacgtcgccg tagcaaataa ttttagagga taagccatgc cacgttctct
     3541 caagaaaggt ccttttattg acctgcactt gctgaagaag gtagagaaag cggtggaaag
     3601 cggagacaag aagcccctgc gcacttggtc ccgtcgttca acgatctttc ctaacatgat
     3661 cggtttgacc atcgctgtcc ataatggtcg tcagcacgtt ccggtatttg taaccgacga
     3721 aatggtcggt cacaaactgg gtgaattcgc accgactcgt acttatcgcg gccacgctgc
     3781 tgataaaaaa gcgaagaaga aataaggtag gaggaagaga tggaaactat cgctaaacat
     3841 cgccatgctc gttcttctgc tcagaaggtt cgccttgttg ctgacctgat tcgcggtaag
     3901 aaagtgtcgc aggctctgga tattttgacc tacaccaaca agaaagcggc tgtactggtc
     3961 aagaaagttc tggaatctgc cattgctaac gctgaacaca acgatggcgc tgacattgac
     4021 gatctgaaag ttacgaaaat tttcgtagac gaaggcccga gcatgaagcg cattatgccg
     4081 cgtgcaaaag gtcgtgcaga tcgcatcctg aagcgcacca gccacatcac tgtggttgtg
     4141 tccgatcgct gagactctgg agactagcaa tgggtcagaa agtacatcct aatggtattc
     4201 gcctgggtat tgtaaaacca tggaactcta cctggtttgc gaacaccaaa gaattcgctg
     4261 acaacctgga cagcgatttt aaagtacgtc agtacctgac taaggaactg gctaaagcgt
     4321 ccgtatctcg tatcgttatc gagcgtccgg ctaagagcat ccgtgtaacc attcacactg
     4381 ctcgcccggg tatcgttatc ggtaaaaaag gtgaagacgt agaaaaactg cgtaaggtcg
     4441 tagcggacat cgctggcgtt cctgcacaga tcaacatcgc cgaagttcgt aagcctgaac
     4501 tggacgcaaa actggttgct gacagcatca cttctcagct ggaacgtcgc gttatgttcc
     4561 gtcgtgctat gaagcgtgct gtacagaacg caatgcgtct gggcgctaaa ggtattaaag
     4621 ttgaagttag cggccgtctg ggcggcgcgg aaatcgcacg taccgaatgg taccgcgaag
     4681 gtcgcgtacc gctgcacact ctgcgtgctg acatcgacta caacacctct gaagcgcaca
     4741 ccacttacgg tgtaatcggc gttaaagtgt ggatcttcaa aggcgagatc ctgggtggta
     4801 tggctgctgt tgaacaaccg gaaaaaccgg ctgctcagcc taaaaagcag cagcgtaaag
     4861 gccgtaaata aggagcgtcg ctgatgttac aaccaaagcg tacaaaattc cgtaaaatgc
     4921 acaaaggccg taaccgcggt ctggcgcagg gtacggatgt tagcttcggc agcttcggtc
     4981 tgaaagctgt tggccgtggt cgtctgactg cccgtcagat cgaagcagca cgtcgtgcta
     5041 tgacccgtgc agttaagcgt caaggtaaga tctggatccg tgtgttcccg gacaaaccga
     5101 tcactgaaaa gccgctggca gtgcgtatgg gtaaaggtaa aggtaacgtg gagtattggg
     5161 ttgccttgat tcagccgggt aaagtcctgt atgaaatgga cggtgttccg gaagagctgg
     5221 cccgtgaagc attcaagctg gcagcagcga aactgccgat taaaaccacc tttgtaacta
     5281 agacggtgat gtaatgaaag caaaagagct gcgtgagaag agcgttgaag agctgaacac
     5341 cgagctgctg aacctgctgc gtgagcagtt caacctgcgt atgcaggctg caagtggcca
     5401 gctgcaacag tctcacctgt tgaagcaagt gcgtcgcgat gtcgcacgcg ttaagacttt
     5461 actgaacgag aaggctggtg cgtaatgacc gataaaatcc gtactctgca aggtcgcgtt
     5521 gttagcgaca aaatggagaa atccattgtt gttgctatcg aacgttttgt gaaacacccg
     5581 atctacggta aattcatcaa gcgtacgacc aaactgcacg tacatgacga gaacaacgaa
     5641 tgcggtatcg gtgacgtggt tgaaatccgc gaatgccgtc cgctgtccaa gactaaatcc
     5701 tggacgctgg ttcgcgttgt agagaaagcg gttctgtaat acagtacact ctctcgatac
     5761 gaataaacgg ctcagaaatg agccgtttat tttttctacc catatccttg aagcggtgtt
     5821 ataatgccgc gccctcgata tggggatttt taacgacctg attttcgggt ctcagtagta
     5881 gttgacatta gcggagcact aaaatgatcc aagaacagac tatgctgaac gtcgccgaca
     5941 actccggtgc acgtcgcgta atgtgtatca aggttctggg tggctcgcac cgtcgctacg
     6001 caggcgtagg cgacatcatc aagatcacca tcaaagaagc aattccgcgt ggtaaggtca
     6061 aaaaaggtga tgtgctgaag gcggtagtgg tgcgcaccaa gaagggtgtt cgtcgcccgg
     6121 acggttctgt cattcgcttc gatggtaatg cttgtgttct tctgaacaac aacagcgagc
     6181 agcctatcgg tacgcgtatt tttgggccgg taactcgtga gcttcgtagt gagaagttca
     6241 tgaaaattat ctctctggca ccagaagtac tctaaggagc gaatcatggc agcgaaaatc
     6301 cgtcgtgatg acgaagttat cgtgttaacc ggtaaagata aaggtaaacg cggtaaagtt
     6361 aagaatgtcc tgtcttccgg caaggtcatt gttgaaggta tcaacctggt taagaaacat
     6421 cagaagccgg ttccggccct gaaccaaccg ggtggcatcg ttgaaaaaga agccgctatt
     6481 caggtttcca acgtagcaat cttcaatgcg gcaaccggca aggctgaccg tgtaggcttt
     6541 agattcgaag acggcaaaaa agtccgtttc ttcaagtcta acagcgaaac tatcaagtaa
     6601 tttggagtag tacgatggcg aaactgcatg attactacaa agacgaagta gttaaaaaac
     6661 tcatgactga gtttaactac aattctgtca tgcaagtccc tcgggtcgag aagatcaccc
     6721 tgaacatggg tgttggtgaa gcgatcgctg acaaaaaact gctggataac gcagcagcag
     6781 acctggcagc aatctccggt caaaaaccgc tgatcaccaa agcacgcaaa tctgttgcag
     6841 gcttcaaaat ccgtcagggc tatccgatcg gctgtaaagt aactctgcgt ggcgaacgca
     6901 tgtgggagtt ctttgagcgc ctgatcacta ttgctgtacc tcgtatccgt gacttccgtg
     6961 gcctgtccgc taagtctttc gacggtcgtg gtaactacag catgggtgtc cgtgagcaga
     7021 tcatcttccc agaaatcgac tacgataaag tcgaccgcgt tcgtggtttg gatattacca
     7081 ttaccactac tgcgaaatct gacgaagaag gccgcgctct gctggctgcc tttgacttcc
     7141 cgttccgcaa gtaaggtagg gttactaaat ggctaagcaa tcaatgaaag cacgcgaagt
     7201 aaaacgcgta gctttagctg ataaatactt cgcgaaacgc gctgaactga aagcgatcat
     7261 ctctgatgtg aacgcttccg acgaagatcg ttggaacgct gttctcaagc tgcagactct
     7321 gccgcgtgat tccagcccgt ctcgtcagcg taaccgctgc cgtcaaacag gtcgtccgca
     7381 tggtttcctg cggaagttcg ggttgagccg tattaaggtc cgtgaagccg ctatgcgcgg
     7441 tgaaatcccg ggtctgaaaa aggctagctg gtaattgtca ccaattgaat cacgggagta
     7501 aagacagatg agcatgcaag atccgatcgc ggatatgctg acccgtatcc gtaacggtca
     7561 ggccgcgaac aaagctgcgg tcaccatgcc ttcctccaag ctgaaagtgg caatcgccaa
     7621 cgtgctgaag gaagaaggtt ttattgaaga ttttaaagtt gaaggcgaca ccaagcctga
     7681 actggaactt actctgaagt atttccaggg caaagctgtt gtagaaagca ttcagcgtgt
     7741 cagccgccca ggtctgcgca tctataaacg taaagatgag ctgccgaaag ttatggcggg
     7801 tctgggtatc gcagttgttt ctacctctaa aggtgttatg actgatcgtg cagcgcgcca
     7861 ggctggtctt ggtggcgaaa ttatctgcta cgtagcctaa tcggaggaaa aaatgtctcg
     7921 tgttgctaaa gcaccggtcg ttgttcctgc cggcgttgac gtaaaaatca acggtcaggt
     7981 tattacgatc aaaggtaaaa acggcgagct gactcgtact ctcaacgatg ctgttgaagt
     8041 taaacatgca gataataccc tgaccttcgg tccgcgtgat ggttacgcag acggttgggc
     8101 acaggctggt accgcgcgtg ccctgctgaa ctcaatggtt atcggtgtta ccgaaggctt
     8161 cactaagaag ctgcagctgg ttggtgtagg ttaccgtgca gcggttaaag gtaatgtgat
     8221 taacctgtct ctgggtttct ctcatcctgt tgaccatcag ctgcctgcgg gtatcactgc
     8281 tgaatgtccg actcagactg aaatcgtgct gaaaggcgct gataagcagg tgatcggcca
     8341 ggttgcagcg gatctgcgcg cctaccgtcg tcctgagcct tataaaggca agggtgttcg
     8401 ttacgccgac gaagtcgtgc gtatcaaaga ggctaagaag aagtaaggta acactatgga
     8461 taagaaatct gctcgtatcc gtcgtgcgac ccgcgcacgc cgcaagctcc aggagctggg
     8521 cgcaactcgc ctggtggtac atcgtacccc gcgtcacatt tacgcacagg taattgcacc
     8581 gaacggttct gaagttctgg tagctgcttc tactgtagaa aaagctatcg ctgaacaact
     8641 gaagtacacc ggtaacaaag acgcggctgc agctgtgggt aaagctgtcg ctgaacgcgc
     8701 tctggaaaaa ggcatcaaag atgtatcctt tgaccgttcc gggttccaat atcatggtcg
     8761 tgtccaggca ctggcagatg ctgcccgtga agctggcctt cagttctaag gtagaggtgt
     8821 aagatggctc acatcgaaaa acaagctggc gaactgcagg aaaagctgat cgcggtaaac
     8881 cgcgtatcta aaaccgttaa aggtggtcgt attttctcct tcacagctct gactgtagtt
     8941 ggcgatggta acggtcgcgt tggttttggt tacggtaaag cgcgtgaagt tccagcagcg
     9001 atccagaaag cgatggaaaa agcccgtcgc aatatgatta acgtcgcgct gaataacggc
     9061 actctgcaac accctgttaa aggtgttcac acgggttctc gcgtattcat gcagccggct
     9121 tccgaaggta ccggtatcat cgccggtggt gcaatgcgcg ccgttctgga agtcgctggg
     9181 gttcataacg ttctggctaa agcatatggt tccaccaacc cgatcaacgt ggttcgtgca
     9241 actattgatg gcctggaaaa tatgaattct ccagaaatgg tcgctgccaa gcgtggtaaa
     9301 tccgttgaag aaattctggg gaaataaacc atggcaaaga ctattaaaat tactcaaacc
     9361 cgcagtgcaa tcggtcgtct gccgaaacac aaggcaacgc tgcttggcct gggtctgcgt
     9421 cgtattggtc acaccgtaga gcgcgaggat actcctgcta ttcgcggtat gatcaacgcg
     9481 gtttccttca tggttaaagt tgaggagtaa gagatgcgtt taaatactct gtctccggcc
     9541 gaaggctcca aaaaggcggg taaacgcttg ggtcgtggta tcggttctgg cctcggtaaa
     9601 accggtggtc gtggtcacaa aggtcagaag tctcgttctg gcggtggcgt acgtcgcggt
     9661 ttcgaggggg gtcagatgcc tctgtaccgt cgtctgccga aattcggctt cacttctcgt
     9721 aaagcagcga ttacagccga agttcgtctg tctgacctgg ctaaagtaga aggcggtgtt
     9781 gtagacctga acacgctgaa agcggctaac attatcggta tccagatcga gttcgcgaaa
     9841 gtgatcctgg ctggcgaagt aacgactccg gtaactgttc gtggcctgcg tgttactaaa
     9901 ggcgctcgtg ctgctatcga agctgctggc ggtaaaatcg aggaataagt agcagatggc
     9961 taaacaaccg ggattagatt ttcaaagtgc caaaggtggc ttaggcgagc tgaaacgcag
    10021 actgctgttt gttatcggtg cgctgattgt gttccgtatt ggctctttta ttccgatccc
    10081 tggtattgat gccgctgtac ttgccaaact gcttgagcaa cagcgaggca ccatcattga
    10141 gatgtttaac atgttctctg gtggtgctct cagccgtgct tctatctttg ctctggggat
    10201 catgccgtat atttcggcgt cgatcattat ccagctgctg acggtggttc acccaacgtt
    10261 ggcggaaatt aagaaagaag gggagtctgg tcgtcgtaag atcagccagt acacccgcta
    10321 cggtactctg gtgctggcaa tattccagtc gatcggtatt gctaccggtc tgccgaatat
    10381 gcctggtatg caaggcctgg tgattaaccc gggctttgca ttctacttca ccgctgttgt
    10441 aagtctggtc acaggaacga tgttcctgat gtggttgggc gaacagatca ctgaacgagg
    10501 tatcggcaac ggtatttcaa tcattatctt cgccggtatt gtcgcgggac tcccgccagc
    10561 cattgcccat actatcgagc aagcgcgtca aggcgacctg cacttcctcg tgttgctgtt
    10621 ggttgcagta ttagtatttg cagtgacgtt ctttgttgta tttgttgagc gtggtcaacg
    10681 ccgcattgtg gtaaactacg cgaaacgtca gcaaggtcgt cgtgtctatg ctgcacagag
    10741 cacacattta ccgctgaaag tgaatatggc gggggtaatc ccggcaatct tcgcttccag
    10801 tattattctg ttcccggcga ccatcgcgtc atggttcggg ggcggtactg gttggaactg
    10861 gctgacaaca atttcgctgt atttgcagcc tgggcaaccg ctttatgtgt tactctatgc
    10921 gtctgcaatc atcttcttct gtttcttcta cacggcgttg gttttcaacc cgcgtgaaac
    10981 agcagataac ctgaagaagt ccggtgcatt tgtaccagga attcgtccgg gagagcaaac
    11041 ggcgaagtat atcgataaag taatgacccg cctgaccctg gttggtgcgc tgtatattac
    11101 ctttatctgc ctgatcccgg agttcatgcg tgatgcaatg aaagtaccgt tctacttcgg
    11161 tgggacctca ctgcttatcg ttgttgtcgt gattatggac tttatggctc aagtgcaaac
    11221 tctgatgatg tccagtcagt atgagtctgc attgaagaag gcgaacctga aaggctacgg
    11281 ccgataattg gtcgcccgag aagttacgga gagtaaaaat gaaagttcgt gcttccgtca
    11341 agaaattatg ccgtaactgc aaaatcgtta agcgtgatgg tgtcatccgt gtgatttgca
    11401 gtgccgagcc gaagcataaa cagcgccaag gctgattttt tcgcatattt ttcttgcaaa
    11461 gttgggttga gctggctaga ttagccagcc aatcttttgt atgtctgtgc gtttccattt
    11521 gagtatcctg aaaacgggct tttcagcatg gaacgtacat attaaatagt aggagtgcat
    11581 agtggcccgt atagcaggca ttaacattcc tgatcataag catgccgtaa tcgcattaac
    11641 ttcgatttat ggcgtcggca agacccgttc taaagccatc ctggctgcag cgggtatcgc
    11701 tgaagatgtt aagatcagtg agctgtctga aggacaaatc gatacgctgc gtgacgaagt
    11761 tgccaaattt gtcgttgaag gtgatctgcg ccgtgaaatc agcatgagca tcaagcgcct
    11821 gatggatctt ggttgctatc gcggcttgcg tcatcgtcgt ggtctcccgg ttcgcggtca
    11881 gcgtaccaag accaacgcac gtacccgtaa gggtccgcgc aaaccgatca agaaataatc
    11941 ggggtgattg aataatggca aaggcaccaa ttcgtgcacg taaacgtgta agaaaacaag
    12001 tctctgacgg cgtggctcat atccatgctt ctttcaacaa caccatcgtg actatcactg
    12061 atcgtcaggg taacgcgttg ggttgggcaa cagccggtgg ttccggtttc cgtggttctc
    12121 gcaaatccac tccgtttgca gctcaggttg cagcagagcg ttgcgctgac gccgtgaaag
    12181 aatacggcat caagaatctg gaagttatgg ttaaaggtcc gggtccaggc cgcgaatcta
    12241 ctattcgtgc tctgaacgcc gcaggtttcc gcatcactaa cattactgat gtgactccga
    12301 tccctcataa cggttgtcgt ccgccgaaaa aacgtcgcgt ataacgcctc gttttccagg
    12361 tttgttggag aaagaaaatg gcaagatatt tgggtcctaa gctcaagctg agccgtcgtg
    12421 agggcaccga cttattcctt aagtctggcg ttcgcgcgat cgataccaag tgtaaaattg
    12481 aacaagctcc tggccagcac ggtgcgcgta aaccgcgtct gtctgactat ggtgtgcagt
    12541 tgcgtgaaaa gcaaaaagtt cgccgtatct atggtgtgct ggagcgtcag ttccgtaact
    12601 actacaaaga agcagcacgt ctgaaaggca acaccggtga aaacctgttg gctctgctgg
    12661 aaggtcgtct ggacaacgtt gtataccgta tgggcttcgg tgccactcgt gcagaagcac
    12721 gtcagctggt tagccataaa gcaattatgg taaacggtcg tgttgttaac atcgcttctt
    12781 atcaggttag tccgaatgac gttgtaagca ttcgtgagaa agcgaagaag cagtctcgcg
    12841 tgaaagccgc tctggagctg gctgagcagc gtgaaaagcc aacttggctg gaagttgatg
    12901 ctggcaagat ggaaggtacg tttaagcgta agccggagcg ttctgatctg tctgcggaca
    12961 ttaacgaaca cctgatcgtc gagctttact ccaagtaaag cttagtacca aagagaggac
    13021 acaatgcagg gttctgtgac agagtttcta aaaccgcgcc tggttgatat cgagcaagtg
    13081 agttcgacgc acgccaaggt gacccttgag cctttagagc gtggctttgg ccatactctg
    13141 ggtaacgcac tgcgccgtat tctgctctca tcgatgccgg gttgcgcggt gaccgaggtt
    13201 gagattgatg gtgtactaca tgagtacagc accaaagaag gcgttcagga agatatcctg
    13261 gaaatcctgc tcaacctgaa agggctggcg gtgagagttc agggcaaaga tgaagttatt
    13321 cttaccttga ataaatctgg cattggccct gtgactgcag ccgatatcac ccacgacggt
    13381 gatgtcgaaa tcgtcaagcc gcagcacgtg atctgccacc tgaccgatga gaacgcgtct
    13441 attagcatgc gtatcaaagt tcagcgcggt cgtggttatg tgccggcttc tacccgaatt
    13501 cattcggaag aagatgagcg cccaatcggc cgtctgctgg tcgacgcatg ctacagccct
    13561 gtggagcgta ttgcctacaa tgttgaagca gcgcgtgtag aacagcgtac cgacctggac
    13621 aagctggtca tcgaaatgga aaccaacggc acaatcgatc ctgaagaggc gattcgtcgt
    13681 gcggcaacca ttctggctga acaactggaa gctttcgttg acttacgtga tgtacgtcag
    13741 cctgaagtga aagaagagaa accagagttc gatccgatcc tgctgcgccc tgttgacgat
    13801 ctggaattga ctgtccgctc tgctaactgc cttaaagcag aagctatcca ctatatcggt
    13861 gatctggtac aacgtaccga ggttgagctc cttaaaacgc ctaaccttgg taaaaaatct
    13921 cttactgaga ttaaagacgt gctggcttcc cgtggactgt ctctgggcat gcgcctggaa
    13981 aactggccac cggcaagcat cgctgacgag taaccggatc acaggttaag gttttactga
    14041 gaaggataag gtcatgcgcc atcgtaagag tggtcgtcaa ctgaaccgca acagcagcca
    14101 tcgccaggct atgttccgca atatggcagg ttcactggtt cgtcatgaaa tcatcaagac
    14161 gactctgcct aaagcgaaag agctgcgccg cgtagttgag ccgctgatta ctcttgccaa
    14221 gactgatagc gttgctaatc gtcgtctggc attcgcccgt actcgtgata acgagatcgt
    14281 ggcaaaactg tttaacgaac tgggcccgcg tttcgcgagc cgtgccggtg gttacactcg
    14341 tattctgaag tgtggcttcc gtgcaggcga caacgcgccg atggcttaca tcgagctggt
    14401 tgatcgttca gagaaagcag aagctgctgc agagtaatct gaagcaacgt aaaaaaaccc
    14461 gccccggcgg gtttttttat acccgtagca tccccactta tctacaatag ctgtactctt
    14521 tttgttcatc ccctggagta tttatgtggt tacttgacca gtgggcagag cgtcatatag
    14581 cagaagcgca agcgaaaggt gagtttgata atctggcagg tagcggcgaa ccattgatac
    14641 tggatgatga ttctcatgtg ccaccggaat tacgtgcggg gtatcgcttg ctgaagaatg
    14701 ctggttgctt accgccagaa cttgagcagc ggagggaagc aattcagctt ctggatatcc
    14761 tgagaggtat ccgtcacgat gatccgcaat atcaagaggt tagccgtcga ttgtcattac
    14821 tggaattgaa gctgcgacaa gctggattta gtaccgattt tttacgcggc gattatgctg
    14881 acaagttgtt ggacaaaatc aacgataact agtggagtat gtatgtatcg cattggtgag
    14941 ctggcaaaaa tggcggaagt aacacctgac acgattcgtt attacgaaaa acagcagatg
    15001 atggagcatg aagtgcgtac tgaaggcggg tttcgcctgt ataccgaaag cgatctccag
    15061 cgattgaaat ttatccgcca tgccagacaa ctaggtttca gtctggagtc gatccgcgag
    15121 ttgctgtcga tccgcatcga tcctgaacac catacctgtc aggagtcaaa aggcattgtg
    15181 caggaaagat tgcaggaagt cgaagcacgg atagccgagt tgcagagtat gcagcgttcc
    15241 ttgcaacgcc ttaacgatgc ctgttgtggg accgcccata gcagtgttta ttgttcgatt
    15301 cttgaagctc ttgaacaagg ggcgagtggc gttaagagtg gctgttgatt ttttgcactg
    15361 gcaggattac actcgcgccg taaaataacc aattggagtt tttatgagcc gatatcagca
    15421 tactaaaggg cagataaggg ataatgcgat agaagcatta ctacatgatc ccttattccg
    15481 acagcgcgta gagaaaaata agaaggggaa aggcagttac atgcgaaaag gaaaacatgg
    15541 caatcggggg aactgggagg ccagtggcaa gaaagtaaat cactttttta ccactggtct
    15601 tctgctttca ggcgcttgtt aagagcggtt attctgctct ttcagcaaat cacgaatttc
    15661 tgccagtaat acttcttctt tagttggtgc aggtgcggca gctggttctt cttttttccg
    15721 attcagtttg ttgattagct taatcgccat aaagatggca aaggccacaa tcagaaaatc
    15781 aaagacgttt tgaatgaaga caccgtaatg catcacaaca gcagggatat ccccctgcgc
    15841 atcgcgtagc gtgacagcaa actgtttaaa gtcgatcccg ccaattaata agcccagagg
    15901 tggcatgatg atatcggcaa ccagtgaaga tacaatcttc ccgaatgccg caccgataat
    15961 gacacccacc gccaaatcca ccacgttccc gcgcatcgca aattcgcgaa attctttaat
    16021 aatgctcatg ttattctccc tatgaagccg acaaccataa gtctaacaaa tgttaagcca
    16081 ttttcctgcc aggaatgaat ttaattaatc cttgataatc aaagggaaat aataaggcgt
    16141 cattagacgc cttattaatt acaagaagaa agggcttggc tggaagagtc tttcgacgtc
    16201 ggtaataaac tttttatctg tgaggaacat aattacgtga tcgccttgct caatgcgcag
    16261 attgtcattg gcaatcatca cgtcgtttcc acgtaccacc gctccaataa tcgttcctgg
    16321 cggtagcttg atttcgtcaa tgactctgcc gacaacgcgt gaggtgcttt catcaccgtg
    16381 agcaacggct tcaatagctt ctgctacgcc gcggcgcaat gaggaaacac caacaatatc
    16441 tgcttttcgc acatggctaa gcaacgcaga aatagttgct tgttgtggtg aaattgcaat
    16501 atcgatcacg ctcccctgaa ccagatccac ataagcgcga cgctggatca ataccatcac
    16561 ctttttcgca cccatacgtt tggcaagcat ggcggacatg atattggcct cgtcatcgtt
    16621 ggtgacggca ataaacagat caacttgatc gatatgttct tcggccagca gttcttgatc
    16681 cgacgcatca ccaaaaaaga cgatcgtatt ctgcaacttt tccgccagtt cggcagcgcg
    16741 ctgctgatta cgttcgatga gtttaacgct gtaatctttt tccagacgac gcgccagccc
    16801 tgcaccgata ttaccgccac caaccagcat gatccgctta tacggttttt ccagtcgctg
    16861 taattcactc atcaccgcgc ggatatgctg tgaagcggca ataaagaaca cttcatcacc
    16921 agcttcaaca atggtcgaac cttgtggacg aatggggcga tcgtggcgga aaattgctgc
    16981 cacacgagta tcgatatgtg gcatatgttc gcgcatggtc gaaagtgcat taccaatcag
    17041 cgggccgcca taataggctt taactacagc gaggctgact ttaccctcag cgaagttcac
    17101 cacctgcaat gcgcctggat actcaatcag tcggtaaata ttatcgataa ccaactgctc
    17161 tggtgcgatc agatgatcaa tcggtacagc atctgaatga aatagcttat cggcatcgcg
    17221 tacgtagtct ggtgagcgga tgcgagcgat gcgattaggg gtgttgaaaa gtgagtaggc
    17281 tacctggcag gcaaccatat tggtttcatc tgaactggtt acagcaacca gcatatcggc
    17341 gtcgtcggca cctgcctccc gcaatacgcg tggatgagag ccatgcccct gcacgactcg
    17401 caggtcaaat ttatcctgca aggtccgcag acgctcaccg ttggtatcga caacagtaat
    17461 atcgttgttc tcgccaacca ggttttccgc cagtgtgccg ccaacctggc cggcacccag
    17521 aatgataatt ttcatcagtc tcgacccgtt atctcatcac tttttgatta gcttagcgta
    17581 aaagaagccg tcgccctctt cggcaccagg cagattttgt ttacccggtt gctctggtgt
    17641 tcctgtttcg caaagttcgg catcagcggt acgttgcaaa aaagctttaa tctgcaggct
    17701 attctcttcc ggtaacaccg aacaggtggc atagaccagg gttccaccgg attttaaatg
    17761 cggccaaatg gcgtcgagaa tttcagactg caattgcgcg agttccggga tatcgcgatc
    17821 gcgacgtaac catttaatat ccggatggcg acgaatcacc cccgttgctg aacaaggcgc
    17881 atctaataaa atgcgatcaa actgttgctc gccacaccat tgggaagggt aacggccatc
    17941 accttgtttc acggttgcct tcataccaag gcgttttaaa ttgtcgtaaa cgcgagagag
    18001 gcgctgttcg tcaatatcaa ccgcaacaac ctgcgcttct ggtgccacct caaggatatg
    18061 cgttgtttta ccgccggggg cggcacaaag atccagaatg tgttcaccgt tttgtggcgc
    18121 aagccaggtc atgcaacctt gtgctgatgc atcctgaacg gtaacccatc cgtcttcaaa
    18181 accaggtagc gcatgaacag gtgcaggtgt ttccagacgt acagcatcag ggtaatccgc
    18241 atgcgggaaa cctttcattc ctgcttcatc cagcaatgca agccagctgt cgcgggaatg
    18301 atgcgtacga ttaacgcgca gccacattgg cggacgctgg ttattggctt cgacgatgga
    18361 ttgccactgc tctggatacg ctttttgcag acgcttcagc aaccaggaag gatgcagata
    18421 acgtgcatca ctggcattaa acttggctaa taactcttct tgctgacgct ggaactggcg
    18481 taatacaccg ttaatcaacc ctttaagttg cggacgctta attgcgatag cgccttcaac
    18541 cgtttcagcc agcgcagcat gaggtggaat gcgggtataa agcagttgat acaaaccaac
    18601 cataatcagg taatgcacag tgcgctgttt gccggtcatc ggacgggcca ttaacttatt
    18661 aatcagccag tctaactgcg aaagcgtacg cagtacgcca aagcacaact cttgaagaag
    18721 tgctttgtct ttatcagaaa ctttttgctg gagcggtggc agaatgttgc ttaatgattg
    18781 cccttgctcg acgacttgtt caacggcctg ggccgccatg ctacgtaaat tacgttgttt
    18841 tttcataagt ataaaaatgc ccggtaagac cgggcttaga agagtggact atcaggccag
    18901 acggttgccc ggaacaaacc attcccgacg agaattcagg aggtcttgcg cgctcatcgc
    18961 tttcttaccc gcaggttgta acgagagcag attcaggatt ccatcaccag ttgcaacctg
    19021 aatgccttgt ttgttggctt caaggatcgt tcctggtgca gcgttggttg ccgtatcaat
    19081 gaccgacgct ttccagactt taaccggctg tctttcaatt tccagccagc tcattggcca
    19141 tggattgaaa gcgcgaatgt agcgttcaag ctgtgctgcc gaaagtgacc agtcaatacg
    19201 cgcttcttct ttactcaact tttcggcgta agtgacaaga gtttcgtcct gaacttctgg
    19261 tttcgccgtg ccgtctgcca gttgtttcaa cgtggtgata agcccttgtg ggccaagctc
    19321 tgccagcttg tcgtacagcg taccactggt atcttctgca gtaatcgggc aggagagctt
    19381 atagagcatg tcaccggtgt ctaaaccgac atccatttgc ataatggtca caccagtttc
    19441 tgcatcaccc gcccatagtg agcgttggat tggtgcagca ccgcgccagc gtggcagcag
    19501 tgaaccatga acgttgatac agccaagacg cggcatctcc agcactgctt taggcagaat
    19561 taagccatag gcgacgacga ccataacatc agcctgcaga tcggcgacca gttgctggtt
    19621 ttcttgtgga cgcagggaaa caggttgaaa aacgggcaga cctttttcct cagccagaac
    19681 tttaaccggg ctgggcatca gttttttacc gcgtcctgcc ggtcggtctg gctgggtgaa
    19741 cacgccaacg acgttatgac cagaagacaa cagcgcgtcg agatgacgcg ctgcaaagtc
    19801 aggtgtaccc gcaaaaataa tacgtagtga ttctgacacg ttagttctta tccttaagcc
    19861 cgggctttca gacgatccag tttttcaact ttctgacgaa tacgttgttg tttcaacggt
    19921 gacagataat ccataaacag tttgccgacc aggtgatcca tctcatgctg aatacagatg
    19981 gctaacagac cgtctgcttc caattcaaat ggtttaccgt cgcggtccag ggcgcgaatt
    20041 ttaactttct ctgcgcgtgg cactaaagca cgttgttcag ggatcgacag gcaaccttct
    20101 tcaatgcctg tttcgccgct tttttctaaa agctccggat tgattaacac cagccgttcg
    20161 tcacggtttt ccgaaacatc aataacaatg atacgttgat ggatatcaac ctgggttgcc
    20221 gccaggccaa taccttcttc tgcgtacatc gtctcgaaca tatcatcgac gatacgctga
    20281 atttctgcat tcacttcttc taccggttta gcaactttgc gaagccgctc gtccggaata
    20341 tgtaacactt gcaaaactga cataaatctc cagagatgtg ttcaggagtt agaaagatta
    20401 tttcttctat tctagacaaa tccccctctg attgacagca tcactgacca atcgcaaaga
    20461 ttgctaaggc tgcttatggc agggagataa ggatggtcga tacagatatt tggctgcgtt
    20521 taatgagcat cagcagcttg tacggcgatg atatggtccg tatagctcac tggctggcaa
    20581 aacagtcgca tattgatgcg gttgtattgc agcaaacagg gcttacattg cggcaggcac
    20641 aacgctttct ttcatttccg cgaaagagta tcgaaagctc actttgttgg ttggagcaac
    20701 ccaaccatca tttaatccct gcggacagcg aattttatcc tcctcaactt ctggcgacga
    20761 cagattaccc cggcgcactg tttgttgaag gagaactgta cgcgctgcat tcatttcagc
    20821 ttgccgtagt ggggagtcgg gcgcattcat ggtatggcga gcgatgggga cgattatttt
    20881 gcgaaactct ggccacgcgt ggagtgacaa ttacgagtgg actggcgcgt ggaatcgatg
    20941 gtgtggcgca taaagcggcc ttacaggtaa atggcgtcag cattgctgta ttggggaacg
    21001 gacttaatac cattcatccg cgccgccatg cccgactggc taccagtttg cttgaacatg
    21061 gtggggcact tgtctcggaa tttcccctcg atgtttcacc ccttgcttac aatttcccac
    21121 gaagaaatcg cattatcagt ggtctaagta aaggtgtact ggtggtggaa gcggctttgc
    21181 gcagtggttc gttggtgaca gcacgttgtg cgcttgagca ggggcgtgaa gtttttgcct
    21241 tgccaggacc aatagggaat ccgggaagcg aagggcctca ctggttaata aaacaaggtg
    21301 cgattcttgt gacggaaccg gaagaaattc tggaaaactt gcaatttgga ttgcactggt
    21361 tgccagacgc ccctgaaaat tcattttatt caccagatca gcaagacgtg gcattgccat
    21421 ttcctgagct cctggctaac gtaggagatg aggtaacacc tgttgacgtc gtcgctgaac
    21481 gtgccggcca acctgtgcca gaggtagtta ctcaactact cgaactggag ttagcaggat
    21541 ggatcgcagc tgtacccggc ggctatgtcc gattgaggag ggcatgccat gttcgacgta
    21601 ctaatgtatt tgtttgaaac ctatattcac acagaagctg agttgcgtgt ggatcaagac
    21661 aaacttgaac aggatcttac cgacgcagga tttgatcgag aagatatcta caatgccctg
    21721 ctttggctgg aaaagcttgc ggattatcag gaaggactga cagaaccgat gcaactggcc
    21781 tctgatccac tctccatgcg tatttatacc cctgaggagt gtgaaagact ggatgccagc
    21841 tgtcgtggtt ttctgctttt ccttgagcaa attcaggtgc ttaaccttga aatccgtgaa
    21901 atggtgatag agcgagtgct cgcgctggat accgcagagt tcgacctgga agacctgaaa
    21961 tgggtaatcc tgatggtgtt gttcaatatt ccgggctgcg aaaatgcgta ccagcaaatg
    22021 gaagaattac tctttgaagt gaatgaaggt atgctgcatt aatcatttct ttaattcagc
    22081 ataagttgtt atggcgaaat cagcactgtt cacggtgcgt aataatgagt cctgcccaaa
    22141 gtgcggggct gaactggtta ttcgatccgg gaaacacggt ccgtttcttg gatgctcaca
    22201 gtatccggcg tgtgactacg tccgtcctct gaaatcttca gcggatggac atattgtcaa
    22261 agttctggag gggcaggttt gccctgcatg tggcgcaaat ctggtattac gccagggacg
    22321 ctttggtatg tttattggtt gcagtaacta ccctgaatgc gaacataccg aacttatcga
    22381 taaaccggac gaaacagcaa ttacatgccc ccaatgtcgg acgggccatc tggtccagcg
    22441 ccgctcccgt tatggcaaaa catttcactc ctgtgatcgc tacccggagt gtcaatttgc
    22501 cattaacttc aaacccatag ctggagaatg ccctgagtgt cattatccac tactcatcga
    22561 aaagaaaacc gcgcagggtg taaaacactt ttgtgccagt aaacaatgtg gaaagccggt
    22621 ttcggcggaa taataacgtg aataataacc tgcaaggaga cgctatcgca gctgcgatag
    22681 atgtcctcaa tgaagaacgt gtcatcgcct atccaacgga agccgttttc ggtgttgggt
    22741 gcgatcctga tagcgaaaca gcagtgatga gactgttgga gttaaaacag cgtccggttg
    22801 ataaggggct gattttaatc gcggcaaatt acgagcagct taaaccctat attgatgaca
    22861 ccatgctgac tgatgcgcag cgtgaaacca ttttttcccg ctggccaggt cctgtcacct
    22921 ttgtctttcc cgcgcctgcg acaacaccgc gctggttgac gggccgcttt gattcgcttg
    22981 ctgtacgagt caccgaccat ccgttggtgg ttgctttgtg ccaggcttat ggtaaaccgc
    23041 tggtttctac cagtgccaac ttgagtggat tgccaccttg tcgaacagta gacgaagttc
    23101 gcgcacaatt tggcgcggcg ttcccggttg tgcttggtga aacggggggg cgtttaaatc
    23161 cttcagaaat ccgcgatgcc ctgacgggtg aactgtttcg acaggggtaa cataatggaa
    23221 acctatgctg tttttggtaa tccgatagcc cacagcaaat cgccattcat tcatcagcaa
    23281 tttgctcagc aactgaatat tgaacatccc tatgggcgcg tgttggcacc catcaatgat
    23341 ttcatcaaca cactgaacgc tttctttagt gctggtggta aaggtgcgaa tgtgacggta
    23401 ccttttaaag aagaggcttt tgccagagcg gatgagctta ctgaacgggc agcgttggct
    23461 ggtgctgtta ataccctcaa gcggttagaa gatggacgct tgcagggtga caataccgat
    23521 ggtataggtt tgttaagcga tctggaacgt ctgtctttta tccgtccggg tttacgtatt
    23581 ctgcttatcg gcgctggtgg agcatctcgc ggcgtactac taccactcct ttccctggac
    23641 tgtgcggtga caatcactaa tcggacggta tcccgtgcgg aagagttggc taaattgttt
    23701 gcgcacactg gcagtattca ggcgttgggt atggacgaac tggaaggtca tgagtttgat
    23761 ctcattatta atgcaacatc cagtggcatc agtggtgata ttccggcgat cccgtcatcg
    23821 ctcattcatc taggcattta ttgctatgac atgttctatc agaaaggaaa aactcctttt
    23881 ctggcatggt gtgagcagcg aggctcaaag cgtaatgctg atggtttagg aatgctggtg
    23941 gcacaggcgg ctcatgcctt tcttctctgg cacggtgttc tgcctgacgt agaaccagtt
    24001 ataaagcaat tgcaggagga attgtccgcg tgaatcaggc catccagttt ccggacaggg
    24061 aagagtggga cgagaataaa aaatgtgtat gttttcccgc tctcgtgaat ggtatgcaac
    24121 tgacatgcgc gatctctggc gagagtctgg cgtatcgctt tactggagat acgccagaac
    24181 agtggttagc gagttttcgt cagcatcgct gggacctgga agaagaagcg gaaaacttaa
    24241 ttcaggaaca aagtgaagat gatcaaggct gggtctggtt accctgatcc agatattcgt
    24301 ccttccattt cacgtaatta ttcgcggaat agcgtaaccc agccttctct tcatcactta
    24361 acgggcggat ctgtttgacg gggctaccga gatacagata tccgctctcc agccgtttat
    24421 tttgtgggac cagactaccc gcaccaatca tcacatcatc ttctactatt gcgccatcaa
    24481 gtaaaattga gcccatccca accaaaactc gattgccaat ggtgcagccg tggagcatca
    24541 ccttgtgacc aacagtgaca tcttcgccaa tggttaatgg gttgccatct gggttgtacg
    24601 aggatttatg agtgacatgc aacatactgc catcctggat attggtgcgt gctccgatct
    24661 gtacataatg tacatctcca cgaatcacaa cgagcggcca gatccccaca tcatcagcca
    24721 gacgaacgcc accaatcacg acactgctat cgtcgatcat tacgcgctga ccgatttgtg
    24781 gaaaaagatc gcggtatggg cgtaaaacat cagacatact tacctcagca ataaatgatt
    24841 tactaatgac tttgggggca ttattggcct tgtgcaagtc ttttagtatg caaaaaagca
    24901 ccgttttgtg tgcgattgca gcaaaaaggg tgaaaaaaca acaaacagaa aaaaagatca
    24961 aaaaaatact tgtgcaaaat attgggatcc ctataatgcg cctccgttaa gacgacaacg
    25021 tgaaacactt cacaggatgg tcggaacaac gaagagaaaa aatcctgaaa ttcagggttg
    25081 actctgaaag aggaaagcgt aatatacgcc acctcgcgac agtgagctga aagcc
//
LOCUS       DADLFA010000042        25119 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000042 DADLFA010000000
VERSION     DADLFA010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25119
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.042"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..393
                     /gene="hyfA"
                     /locus_tag="JQH65_001473"
     CDS             <1..393
                     /gene="hyfA"
                     /locus_tag="JQH65_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HAY6138941.1"
                     /translation="QLNESLCIGCKLCAVVCPFGAISASGSRPVNAPAQYVFQADGSL
                     KDGEENAPTQHALLRWEPGVQTVAVKCDLCNFLPEGPACVRACPNQALRLITDDSLQR
                     QMKEKQRLAASWFANGGEDPLSLTQEQH"
     gene            393..2410
                     /gene="hyfB"
                     /locus_tag="JQH65_001474"
                     /pseudo
     CDS             393..2410
                     /gene="hyfB"
                     /locus_tag="JQH65_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            2421..3368
                     /gene="hyfC"
                     /locus_tag="JQH65_001475"
     CDS             2421..3368
                     /gene="hyfC"
                     /locus_tag="JQH65_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HAY6138942.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLLLTPLFTGISRQIRARMHSRRG
                     PGIWQDYREIHKLFKRQEVAPISSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            3385..3495
                     /locus_tag="JQH65_001476"
     CDS             3385..3495
                     /locus_tag="JQH65_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoin racemase"
                     /protein_id="HAY6138943.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGFCSPH"
     gene            3507..4614
                     /locus_tag="JQH65_001477"
                     /pseudo
     CDS             3507..4614
                     /locus_tag="JQH65_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442177.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit D"
     gene            4626..5276
                     /gene="hyfE"
                     /locus_tag="JQH65_001478"
     CDS             4626..5276
                     /gene="hyfE"
                     /locus_tag="JQH65_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HAY6138944.1"
                     /translation="MIGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            5281..6861
                     /gene="hyfF"
                     /locus_tag="JQH65_001479"
     CDS             5281..6861
                     /gene="hyfF"
                     /locus_tag="JQH65_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HAY6138945.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVDVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            6851..8566
                     /gene="hyfG"
                     /locus_tag="JQH65_001480"
     CDS             6851..8566
                     /gene="hyfG"
                     /locus_tag="JQH65_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HAY6138946.1"
                     /translation="MNVNSSSNSGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLHKDAMDYRL
                     RPEPTTDSETYAFINEGNSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            8576..9115
                     /gene="hyfH"
                     /locus_tag="JQH65_001481"
     CDS             8576..9115
                     /gene="hyfH"
                     /locus_tag="JQH65_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HAY6138947.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTNKA
                     DLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLRAQASVCPECKQ
                     RATLINDDTDVPLVAKEQL"
     gene            9112..9870
                     /gene="hyfI"
                     /locus_tag="JQH65_001482"
     CDS             9112..9870
                     /gene="hyfI"
                     /locus_tag="JQH65_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HAY6138948.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKRHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            9863..10276
                     /gene="hyfJ"
                     /locus_tag="JQH65_001483"
     CDS             9863..10276
                     /gene="hyfJ"
                     /locus_tag="JQH65_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HAY6138949.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHRSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTENEDWLAQVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFALLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            10306..12318
                     /locus_tag="JQH65_001484"
                     /pseudo
     CDS             10306..12318
                     /locus_tag="JQH65_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416986.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
     gene            12340..13046
                     /locus_tag="JQH65_001485"
                     /pseudo
     CDS             12340..13046
                     /locus_tag="JQH65_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001698835.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            12997..13185
                     /locus_tag="JQH65_001486"
     CDS             12997..13185
                     /locus_tag="JQH65_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
                     /protein_id="HAY6138950.1"
                     /translation="MPFAISPPPFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIG
                     GAVLVSMCYRAIYLRQES"
     gene            complement(13223..14284)
                     /locus_tag="JQH65_001487"
     CDS             complement(13223..14284)
                     /locus_tag="JQH65_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAY6138951.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            14497..15960
                     /gene="bepA"
                     /locus_tag="JQH65_001488"
     CDS             14497..15960
                     /gene="bepA"
                     /locus_tag="JQH65_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HAY6138952.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADNADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHLTQRHLARAMEDQQRSTPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            15981..16340
                     /gene="arsC"
                     /locus_tag="JQH65_001489"
     CDS             15981..16340
                     /gene="arsC"
                     /locus_tag="JQH65_001489"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HAY6138953.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(16478..17224)
                     /gene="hda"
                     /locus_tag="JQH65_001490"
     CDS             complement(16478..17224)
                     /gene="hda"
                     /locus_tag="JQH65_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HAY6138954.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(17274..18563)
                     /gene="uraA"
                     /locus_tag="JQH65_001491"
     CDS             complement(17274..18563)
                     /gene="uraA"
                     /locus_tag="JQH65_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAY6138955.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEEADITDK"
     gene            complement(18649..19275)
                     /gene="upp"
                     /locus_tag="JQH65_001492"
     CDS             complement(18649..19275)
                     /gene="upp"
                     /locus_tag="JQH65_001492"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAY6138956.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            19599..20636
                     /gene="purM"
                     /locus_tag="JQH65_001493"
     CDS             19599..20636
                     /gene="purM"
                     /locus_tag="JQH65_001493"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAY6138957.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            20636..21274
                     /gene="purN"
                     /locus_tag="JQH65_001494"
     CDS             20636..21274
                     /gene="purN"
                     /locus_tag="JQH65_001494"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAY6138958.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDVTARVQTQEHAIYPLVISWFADGRLKIHENAAWLDGQRLPPQGYAADE"
     gene            21446..23512
                     /gene="ppk1"
                     /locus_tag="JQH65_001495"
     CDS             21446..23512
                     /gene="ppk1"
                     /locus_tag="JQH65_001495"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HAY6138959.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVKFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNPEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPHLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            23517..25058
                     /gene="ppx"
                     /locus_tag="JQH65_001496"
     CDS             23517..25058
                     /gene="ppx"
                     /locus_tag="JQH65_001496"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HAY6138960.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRVEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
BASE COUNT         5718 a         6577 c         6684 g         6140 t
ORIGIN      
        1 caactcaacg aaagcctctg tattggctgc aagctttgcg ccgtggtctg cccatttggc
       61 gcaatcagcg cttcaggaag ccgtccggtg aatgcccctg cgcaatatgt ttttcaggct
      121 gacggctcac tcaaagatgg cgaagaaaac gcgccgaccc aacatgcttt gctgcgctgg
      181 gaaccgggtg tccagaccgt cgcggtgaaa tgcgacctgt gtaatttctt gccagaaggt
      241 ccggcctgcg ttcgcgcttg cccgaatcag gcgttacggc tgattaccga cgatagcctg
      301 caacgtcaga tgaaagaaaa acagcgcctt gccgcaagct ggtttgccaa tggcggggag
      361 gatccccttt ccctcactca ggagcaacac taatggatgc cctgcaatta ttaacctggt
      421 cgctgatcct ctttctgttt gccagtctgg cttcgctgtt tttactcggt ctggacagac
      481 tggctattaa gctttccggc atcacatcgc tggtgggcgg cgtgattggc atcatcagcg
      541 gaattacgca attacatgct ggtgtaactt tagtcgcccg ttttgccacc ccttttgact
      601 ttgccgattt aaccctgcga atggatagcc tctcggtatt tatggtgctg gttatctcct
      661 tgctggtggt ggtttgttcg ctctattcat tgacttatat gcgcgaatac gagggcaaag
      721 gcgcggcggc gatgggcttc tttatgaatc ttttcatcgc atcgatggtt gccctgctgg
      781 tgatggacaa cgctttttgg ttcatcgtgc tgtttgaaat gatgtcgctg tcttcctggt
      841 ttctggtcat tgccaggcag gataaaacgt cgatcaacgc tggcatgctc tactttttta
      901 tcgcccacgc cggatcggtg ctgattatga tcgccttctt gctgatgggg cgcgaaagcg
      961 gcagcctcga ttttgccagt ttcgcacgct ttcactttct ccggggctgg cgtcggcgat
     1021 gtttctgctg gcctttttcg gttttggcgc gaaagccggg atgatgccgt tgcacagctg
     1081 gttgccgcgc gctcaccctg ccgcaccatc gcacgcttcg gcgttgatgt ctggcgtaat
     1141 ggtcaaaatc ggtattttcg gcatcctgaa agtagcgatg gatctgctgg cgcaaacggg
     1201 tttgcctctg tggtggggca ttctggtgat ggcgatcggc gcaatctccg cgctcctggg
     1261 cgtgctatat gcgctggcgg aacaggatat caaacggctg ctggcctgga gtaccgtcga
     1321 aaacgtcggc attattttgc tggcagtcgg tgtggcgatg gacggtctgt cactgcacga
     1381 cccgctgctc accgtggttg gactgctcgg cgcactgttt catctgctca accatgcgct
     1441 gttcaaaggg ctgctatttc tcggcgcggg cgcgattatt tcgcgtttgc atacccacga
     1501 catggaaaaa atgggggcac tggcgaaacg gatgccgtgg acagccgcag catgcctgat
     1561 tggttgcctc gcgatatcag ccattcctcc gctgaatggt tttatcagcg aatggtacac
     1621 ctggcagtcg ctgttctcac taagtcgtgt ggaagccgta gcgctacaac ttgcgggtcc
     1681 tattgctatg gtaatgctgg cagtcactgg tgggctggca gtaatgtgct tcgtcaaaat
     1741 gtacggtatt actttctgtg gtgcgccgcg cagtacacac gctgaagagg cacaggaagt
     1801 gccaaatacg atgatcgtcg ccatgctact gctcgcggca ctctgcgtat taattgcgct
     1861 tagtgccagt tggctggcac cgaagataat gcatattgcc catgcgttta ccaatacccc
     1921 tcccgtcact gtcgccagcg gaatagcact tgtacccggc acgtttcata cacgggtcac
     1981 tccttcatta ctgttgctgt tactactggc gatgcctttg ctgcctggcc tttactggct
     2041 gtggtgtcgt tcgcgccgcg cagcgtttcg tcgcacagga gatgcctggg catgcggcta
     2101 cggctgggaa aacgcgatgg ctccgtcagg caatggcgtg atgcagccgc tgcgtgtggt
     2161 cttctctgcg ttatttcgtc tacgacaaca gctcgaccct acgctgaggc taaataaaga
     2221 tcttgcgcac gtcaccgcca gggctcagag cacagaaccc ttctgggatg agcgggtgat
     2281 ccgccccatc gtgagcgcca cccaacggct ggccaaagaa atacagcatc tgcaaagcgg
     2341 cgactttcgt ctctattgcc tgtatctagt tgccgcactg gttgtgctgc taatcgctat
     2401 tgccgtctaa ggaaatcacc atgagacaaa ctctttgcga cggatatctg gtcatttttg
     2461 cgttagcaca ggccgtgatt ctgctgctgc taaccccgct ttttacgggt atttcccggc
     2521 agatacgcgc gcgtatgcac tcccgccgcg ggccggggat ctggcaggat tatcgcgaga
     2581 tccacaaact gtttaaacgc caggaagttg cgcctatatc ttcaggtctg atgttccgcc
     2641 tgatgccgtg ggtattaatc agcagcatgc tggtgctggc gatggcccta ccacttttta
     2701 ttaccgtttc cccttttgcg ggcggcggcg atctgatcac ccttatctat cttcttgccc
     2761 tgtttcgttt tttctttgct ctttccggac tggataccgg aagcccgttt gcgggagtcg
     2821 gtgccagtcg cgagttgacg ctcggcattc tggtcgaacc catgctcatt ctctcactgc
     2881 tggtattggc gctgatagca ggttccacgc atatcgagat gatcagcaat acgctggcaa
     2941 cgggctggaa ctcgccgcta accaccgtac tggcgttact ggcctgtggt tttgcctgct
     3001 tcattgagat gggaaaaatt ccctttgatg ttgctgaagc agaacaggaa ttacaggaag
     3061 gcccgctgac cgaatattcc ggtgccgggc tggcgctagc gaaatggggg ctggggctga
     3121 aacaggtcgt gatggcagca ctgtttgtgg ccctgtttct gccctttggg cgcgcgcaag
     3181 aactttctct cgcctgcctg ctgacttcac ttgtcgttac gctgctcaag gttttgctga
     3241 tttttgtact ggcctcaatc gcagaaaaca cgctggcacg cgggcgtttt ttactcattc
     3301 accatgtgac ctggcttggc ttcagccttg ctgcgcttgc atgggtcttc tggttaaccg
     3361 gtctgtaagg agcactgacg gaatatggaa aatcttgctc tgacgacgtt aatgctgcct
     3421 tttatcggcg cactggtcgt ttcgttttcg ccacaacgtc gggccgccga atgggggttt
     3481 tgttcgccgc actgaccacg ctgtgcatgt tgtcgctgat ctccgcgttt tatcaggccg
     3541 ataaagttgc cgtcacgttg acgttggtca acgtggggga tgtggcattg tttggcctgg
     3601 tcattgatcg cgtgagtacg ctgattctgt ttgtggtggt gtttctcggt ttgctggtca
     3661 cgatctactc cacgggttat ctgacggata aaaatcgcga acacccgcat aacggcacga
     3721 atcgttatta cgcatttttg ctggtgttta tcggcgcgat ggcgggactg gtactctcct
     3781 cgacgctgct cggtcagttg ttgttttttg aaattagggc ggctgctcct gggcgttgat
     3841 cagttattac cagagcgata aagcgcagcg ttcaacacta aaagcgttgc ttatcaccca
     3901 tatcggttcg ttggggttgt atcttgccgc cgccacgctg tttttgcaga ccggaacgtt
     3961 tgtgcttagc gcgatgagcg agttacatgg cgacgcacgt tatctggttt atggcggcat
     4021 cctgtttgcc gcgtggggga aatcggccca gctaccgatg caagcgtggc taccggacgc
     4081 aatggaagcg ccaacaccga tcagagccta tctccacgcc gcatcgatgg tgaaagtggg
     4141 cggctggcac tggagggcag catcgcctac atcgtcaacc acgcgttcgc taaaagcctg
     4201 tttttccttg tagcaggtgc gctgagttac agctgcggca cgcgcttgtt gccgcgtctg
     4261 cgtggcgtat tgcactccct gccgttgcca ggcgtgggtt tctgcgtggc agcgctggcg
     4321 attaccggtg tgccgccgtt caacggcttc ttcagtaaat tcccgctgtt tgccgccggt
     4381 tttgcgttgt cagtggagta ctggatcctg ctgcccgcca tgattctgct gatgattgaa
     4441 tcggtcgcca gtttcgcctg gtttattcgc tggtttggtc gcgttgtgcc tggcaaaccg
     4501 agcgaggccg tcgccgatgc cgcaccgctg ccagggtcaa tgcgcctggt gttgattgta
     4561 ctgattgtga tgtcgctgat ttccagcgta atcgccgcga cctggttgca gtaaggagat
     4621 gatgaatgat cggttctatg atcgtaaata atctggcggg actgatgatg ctgacatcgc
     4681 tgtttgtgat tagcgtcaaa agctatcgcc tgtcatgcgg attttacgcc tgccagtcgc
     4741 tggtgctggt gtctattttc gccactctct cgtgcctgtt cgccgcagag caactgctga
     4801 tctggtccgc cagcgccttt atcaccaaag tgctgctggt accgttaatc atgacttacg
     4861 ctgcacgaaa tattccccag aacatcccgg aaaaagcgtt attcggtccg gcaatgatgg
     4921 cactgctcgc ggcgttaatt gtcctgcttt gcgcatttgt cgttcagccc gtgaagctac
     4981 cgatggctac cgggctgaaa ccggcgctgg cggtagcgtt aggtcatttt ctgcttggcc
     5041 tgctgtgcat tgtcagccag cgcaatatcc tgcggcaaat ttttggttac tgcctgatgg
     5101 aaaacggctc ccatctggtg ctggcgcttc ttgcctggcg agcaccggaa ctggtggaaa
     5161 taggtatcgc taccgacgcc atcttcgccg tcattgtgat ggtgttactg gcaagaaaaa
     5221 tatggcgtac ccacggcacg ctggacgtga acaacttgac cgcgctgaag ggataatgag
     5281 atgagttatt ctgtgatgtt cgctttactc ctgctcacgc cgctgctttt ttcgctgctc
     5341 tgttttgcct gccggaaacg ggggctctct gcgacttgta cggtgactgt attacatagc
     5401 ttagggatca cactgctgct gattctggca ctctgggtgg tccaaactgc cgctgatgca
     5461 ggagaaatat tcgctgcggg actgtggctt catattgatg gtctgggcgg tttgttcctc
     5521 gccattcttg gtgtgattgg ctttctcacc ggtgtttact cgattggcta catgcgtcat
     5581 gaagtggcac acggcgagct ttcacccgtt acactgtgcg attactacgg tttcttccat
     5641 ctgtttttgt tcaccatgct gctggttgtt accagcaata acctgattgt gatgtgggcg
     5701 gcgatcgaag ccaccacctt aagctcggcg tttctggtag gcatttacgg tcagcgttca
     5761 tcgctggaat cagcatggaa gtacatcatt atttgtactg ttgatgtcgc ttttggtctg
     5821 ttcggtaccg tgctggtata cgccaacgcc gccagcgtta tgccgcaggc agaaatggcg
     5881 atattctgga gcgaggttct taagcaatcg tccttgcttg acccaacatt aatgctgttg
     5941 gcctttgtgt ttgtgctaat tggctttggt accaaaaccg ggctatttcc catgcacgcc
     6001 tggctgccgg atgctcacag tgaagcgccg agtccggtca gcgccctgct ctccgccgta
     6061 ttgctgaact gcgcgctgtt ggtgctgatt cgctattaca tcattatttg ccaagccatc
     6121 ggcagcgatt tccccaaccg gttgttgctc atcttcggca tgttgtcggt tgccgtggcg
     6181 gcatttttca ttctggtaca gcgggacatt aagcgtctgc tggcgtactc cagcgtggag
     6241 aacatggggc tggtcgcggt ggcgctaggc attggcgggc cgctgggaat ttttgccgcg
     6301 ctgctgcaca ccttaaacca cagtctggca aaaacgctgc tgttctgcgg ttccggcaat
     6361 gtactgctca agtacggcac gcgcgatctc aacgtcgtct gtgggatgct caaaatcatg
     6421 ccatttaccg ccgtgctgtt tggcggcggt gcgctggcgc tggcagggat gccgcccttc
     6481 aacatttttc ttagcgaatt tatgaccgtt accgcaggac tggcacgtaa tcacctgctg
     6541 attatcgtcc tgctgttatt gctgttaacg ctggtgctgg cgggcctggt acggatggct
     6601 gcgcgggtgt taatggcgaa accgccgcag gccgttaacc ggggtgatct cggctggttg
     6661 accacctcgc caatggtgat tctgctggtc atgatgctgg cgatgggaac gcatattcca
     6721 caacctgtca tcaggatcct ggcgggcgct tccactatag tcctctcagg gacgcacgac
     6781 ctgcctgcac aacgtagcac ctggcatgat tttttgcctt caggcaccgc atctgtttcg
     6841 gagaaacaca gtgaacgtta attcatcgtc aaatagtggc gaagcgattc tcgccgccct
     6901 gaaaacgcag ttccccggcg cggtactgga tgaagagcga caaacgcctg aacaggtcac
     6961 cattacggtg aaaatcaatc tgctgcctga cgttgtacat tatctttatt atcaacatga
     7021 tggctggctt ccagtcctgt ttggcaacga cgagcggaca cttaacggtc attacgcggt
     7081 ttattatgcc ctttcaatgg aaggggccga aaaatgctgg atcgtggtga aggcactggt
     7141 cgatgccgac agtcgggagt ttccgtcagt cacaccgcgc gtccctgccg cggtctgggg
     7201 cgagcgagaa attcgcgata tgtacgggct gattccggtt ggcctgccgg atcagcgtcg
     7261 cctggtgttg cccgatgact ggccggaaga tatgcatccg ctgcacaaag atgcgatgga
     7321 ttatcgactg cgccctgaac cgacgacaga ttccgaaacg tatgcgttta tcaatgaggg
     7381 caacagcgat gcgcgggtga tccctgtcgg cccgctgcat atcacctccg atgaaccggg
     7441 tcacttccgc ttgtttgtgg atggcgagca aattgtcgat gctgattacc gcctgtttta
     7501 tgtccatcga ggcatggaga aactggcaga aacgcggatg ggctacaacg aagtgacctt
     7561 cttatcggac cgcgtgtgtg ggatttgcgg ttttgcccac agtgtggcct ataccaactc
     7621 ggttgaaaat gcactgggga ttgaggtgcc gcaacgagcg cataccattc gctcgattct
     7681 gctggaagtc gaacggctac acagtcattt gctcaacctt ggcctctcct gccattttgt
     7741 tggttttgat accggcttta tgcaattttt ccgcgtgcgg gaaaagtcga tgacgatggc
     7801 ggaattgctg accgggtcgc gtaaaaccta cggtctgaat ctgattggtg gtgttcgccg
     7861 cgatattctc aaagagcaac gtctgcaaac gctgaaactg gtgcgcgaga tgcgcgccga
     7921 cgtgtcggag ctggtagaga tgctgcttgc tacgccgaat atggaacaac gcactcaggg
     7981 cattggcatt ctcgaccgac aaatcgcccg tgattatagc cctgtagggc cgctgatccg
     8041 cggcagtggt tttgcccgtg atttgcgctt tgatcacccc tacgccgact acggcaatat
     8101 tccaaaaaca ctgtttacct tcaccggcgg cgatgttttc tcccgcgtga tggtccgtgt
     8161 caaagagacg tttgattcgc tggcaatgct ggaatttgcc ctcgacaaca tgccggatac
     8221 cccactgctg accgaaggct ttagctataa acctcacgca ttcgcgctgg gctttgttga
     8281 agcgccacgc ggtgaagacg tgcactggag catgctcggt gataaccaaa aattgttccg
     8341 ctggcgctgc cgtgccgcca cctacgccaa ctggccggtg ttgcgttaca tgctgcgcgg
     8401 caataccgtt tctgacgcac cgctgattat cggtagcctt gatccctgct actcctgtac
     8461 cgaccgtgtg acgctggtag atgtgcgcaa gcgccagtca aaaaccgtgc cgtataaaga
     8521 gatcgaacgc tacggcattg atcgtaaccg ttcgccgctg aagtaaggac agaagatgct
     8581 gaagttactg aaaactatta tgcgcgccgg aaccgcgacg gtgaaatatc ccttcgcgcc
     8641 actggaggtc agcccaggct ttcgcgggaa accggacctg atgcccagcc aatgtattgc
     8701 ctgcggcgcc tgtgcttgtc cggcaaatgc gctgactatc cagaccgacg accagcaaaa
     8761 ttcgcgcacc tggcagctct atctggggcg ttgtatttac tgcggacgtt gtgaagaagt
     8821 gtgcccgacc agagccatcc agcttaccaa taactttgaa ctgaccgtca ccaataaagc
     8881 cgatctctat acccgcgcga cgttccatct acaacgttgc agccgttgcg aacgcccgtt
     8941 tgccccgcaa aaaaccgtcg cactggctgc tgaattgttg gcacagcaac aaaatgcgcc
     9001 acaaaaccgc gaaatgttgc gggcgcaagc gagcgtctgc ccggagtgca aacaacgcgc
     9061 gacgctgatc aacgacgata cagatgtacc gctggtggct aaggagcagc tatgagtcca
     9121 gtgcttacac aacatgtcag ccagcccatc acgctggatg agcaaacgca aaagatgaag
     9181 cggcatttgc tacaggatat ccgtcgctca gcctacgttt atcgcgtcga ttgcggcggc
     9241 tgcaacgcct gtgaaatcga aatttttgct gccatcaccc ccgtattcga cgcagaacgt
     9301 tttggcatta aggttgtttc atcaccgcgt cacgccgata ttttgttatt tactggcgca
     9361 gtcacccggg cgatgcgtat gcctgcactt cgggcgtatg agtctgcccc cgatcataaa
     9421 atctgtgttt cctacggcgc gtgcggtgtc ggcggcggta ttttccacga tctctacagc
     9481 gtctggggcg gcagcgacac cattgtcccc attgatgttt ggatccccgg ctgcccgcca
     9541 acaccggccg ccaccattca cggtttcgcc gtggcgctcg gtttgctgca acagaagatt
     9601 cacgctgtgg attatcgcga tcccaccggg gtgactatgc aaccgttgtg gccacagatc
     9661 ccgccatcac agcgtatcgc cattgagcga gaagcgcggc ggctggcggg ctatcgtcag
     9721 gggcgagaaa tttgcgatcg gctcctgcgc catttaagcg acgatcctac aggaaatcgg
     9781 gttaacacct ggttgcgcga tgccgacgat ccacgtctca atagtatcgt tcagcaactc
     9841 tttcgcgtac tccgggggtt acatgactga agagtgcggg gaaattgttt tctggacgct
     9901 gcgaaaaaag tttgtcgcca gtagcgacga gatgccggaa caccgctctc aggtaatgta
     9961 ttactcgctg gctatcggcc atcacgttgg cgtgattgat tgtctgaatg tcgccttccg
    10021 ctgcccactg acggaaaacg aagattggct tgcacaggtc gaagaggagc aagcccgacg
    10081 taagatgctg ggggtgatga cctttggtga gattgttatt gacgccagcc acaccgccct
    10141 gttgacccgg gcattcgcgc tactggcaga tgacgcgacg tctgtgtggc aggcgcgtag
    10201 cattcaattc attcatctgt tggatgaaat tgtgcaggaa ccggccatct atctgatggc
    10261 cagaaaaatt gcgtgagaag gatttctcat taataaggac tgttgatggc tatgtcagac
    10321 gaggcgatgt ttgccccgcc acaaggaata acaattgaag cggtaaacgg aatgctcgcg
    10381 gagcggttag cacagaaaca cggtaaggcg tctttattac gcgccttcat cccgatgccg
    10441 ccgccgttca gcccggtaca acttattgaa ctgcatgtta tcaaaagcaa cttctattac
    10501 cgctaccatg atgatggcag cgatgtgacg gcaacaacag agtatcaggg cgagatggtc
    10561 gattattcgc gtcacgccgt ccttctcggc agtagtggaa tggcggagct acgctttatt
    10621 cgtacccacg gcagccgttt tactccctag gattgcacac tgtttaactg gctggcacgc
    10681 attatcactc cggttctgca atcatggctc aatgatgaag aacaacaggt ggcgctgcgt
    10741 ttgctggaga aagatcgcga tcatcatcgg gtactggttg atattactaa tgcagtgctg
    10801 tcacatcttg atctcgacga tctgatcgct gacgtcgctc gtgagatcca tcattttttc
    10861 ggtctggctt cagtcagtat ggtactgggc gatcatcgaa agaacgagaa gtttagcctg
    10921 tggtgcagcg atctttctgc ctcacattgt gcgtgtctgc cacgcaatat gcctggcgac
    10981 agtgtattgc tgacacaaac gctacaaacc cgacaaccga ccttgacgca ccgtgcagac
    11041 gatctgtttc tctggcaacg cgacccgtta ttactcttac ttgcatctaa cggctgcgaa
    11101 tctgcgctcc ttataccgct tacctttggc aaccatacac cgggtgcatt gttgctggcg
    11161 catacctctt ccactctctt tagtgaggaa aactgccagc tactacaaca catagccgat
    11221 cgcatcgcta ttgccgttgg caatgccgat gcctggcgta gcatgaccga tttgcaggaa
    11281 agtttgcagc aagaaaacta ccagcttagc gagcagctcc tttcgaatct gggcatcggt
    11341 gacattatct atcaaagcca ggcaatggaa gacctgctcc agcaggtaga tattgtagcg
    11401 aagagcgaca gtacggtgtt gatttgtggt gaaaccggaa ccggcaaaga ggtgatcgcc
    11461 agagcgctcc atcaacttag cacgcgacgc gacaaaccgc tggtcaaaat caactgcgct
    11521 gccatccccg ccagtcttct ggaaagtgag ttattcggtc atgacaaagg ggcgtttact
    11581 ggtgcgatta atacacatcg tggtcgtttt gaaattgccg atggcggcac gttgtttctc
    11641 gatgaaattg gcgatctgcc gttagaactt cagcctaaac tgctgcgcgt attgcaggaa
    11701 cgggagattg agcgtctcgg cgggagtaga acgatcccgg tgaatgtcag agtcattgcc
    11761 gccaccaacc gtgatttgtg gcaaatggtt gaagatcgcc agtttcgcag caatctcttt
    11821 tatcgcctga atgtcttccc attggaattg ccgccgctac gcgaccgtcc ggaagatatc
    11881 cctcttttag caaaacattt cacgcaaaaa atggcgcgcc atatgaatcg cgcaattgac
    11941 gccatcccga ccgaggcgct acgccagttg atgtcgtggg actggccggg caacgtgcac
    12001 gagctggaaa acgtgattga gcgagcggta ctgctgactc gcggtaacag tctgaattta
    12061 catcttaatg tccgacaaag ccgtttactg ccgacgctaa atgaagattc agcgcttcgc
    12121 agttcaatgg cgcagttgct gcacccgacg acgccagaga atgacgaaga agaacgtcag
    12181 cgcattgttc aggtattgcg agaaaccaat ggcattgttg ccgggccccg tggcgcggcg
    12241 acacgattag ggatgaagcg caccacgctg ctgtcacgaa tgcagcgtct ggggatctcg
    12301 gttcgcgagg tgttgtaatc tgcttttgca ggagtatgca tgagaaacaa actctctttc
    12361 gacttgcagt tgagcgccag aaaagcggca atcgctgaac ggattgccgt ccataaaatt
    12421 gcccgcagta aagtgtcggt ctttttaatg gcgatgtccg ctggcgtgtt tatggcgatc
    12481 ggatttactt tttacctttc cgttatcgcc gatgccccgt cttcacaggc attaacccat
    12541 ctggtgggcg gcctttgctt tacactcggc tttattttgc tggcggtttg cggcacagcc
    12601 tgttcacctc gtcggtaatg acggtgatag caaaaagtcg gggcgttatt agttggcgaa
    12661 cttggctgat taacgcactt ctggtggcct gtggtaatct ggcaggtatt gcctgtttca
    12721 gtttgttaat ctggttttcc gggctggtga tgagtgaaaa cgcgatgtgg ggagtcgcgg
    12781 ttttacactg cgccgagggc aaaatgcatc atacatttac tgaatctgtc agcctcggca
    12841 ttatgtgcaa tctgatggtt tgcctggcgc tgtggatgag ttattgcggg cgttcgttat
    12901 gcgacaaaat cgtcgccatg attttgccca tcaccctgtt tgtcgccagt ggctttgagc
    12961 actgtatcgc caatttgttt gtgattccgt tcgccattgc cattcgccat ttcgcccccc
    13021 cccttctggc aactggcgca cagtagcgca gacaattttc cggcactgac ggtcagccat
    13081 tttattaccg ccaatctgct cccggtgatg ctgggtaata ttatcggcgg tgcagtgctg
    13141 gtgagtatgt gttatcgggc tatttattta cgtcaggaat cctgaaaaat cagcccggcg
    13201 aaacagttcg tcgggctgaa gattattctt gcgcgatttg cccatcgggc caggcgtgga
    13261 tcacggcttt gatcagcgtc gccaatggaa tggcgaagaa tacgccccag aatccccaca
    13321 aaccaccgaa gatcaccacc gataaaataa tcaccagcgg atgcaggtta accgcttcgg
    13381 agaacaacac cggtactagc aggttgccgt ccagcgcctg aataatcaga tacaccgcga
    13441 agcagctcca gaattccgtg cctgcgccaa actggaatag cgccacgcca accaccggaa
    13501 tggtcaccac aaatgcgcca atgtacggaa taagaaccga gaagccgacc agcaccgcca
    13561 gcagcagcga atagttcagc ccaaagagca agaaccccag ccaggtggcg atccccacca
    13621 cgatcatctc cagcactttg ccgcggatat agttggtgat ttgttgattc atctccttcc
    13681 acacctgtcc tgccagtcca cggttgcgcg gcagcacccg acgaacggcg ttcagcatct
    13741 gctctttgtc tttcagcagg aagaagacca tcaatggcac cagcaccaga tagacggcaa
    13801 tggtcagcaa accgaccagc gaggcgaggg aaattttcac caccgaatcg cccatggtca
    13861 gcatccgact gcgcatattt tcggccattg catcaataat gcccgcgtcc attaacgccg
    13921 gatagcggcg cggcaacgtg gcggcaaagt cagaaagctt attgagcatc cccggcatat
    13981 cgcggattaa gtagatgccc tgttgccagg cgataggcag taccacgaac gccatcagta
    14041 gcaatatacc gacaaaaacc accaatacaa tcgacgtcgc ccagcggcgg gagcagccaa
    14101 tagattgcag gcgcacggtt ggccattcca gcaaataggc cagcacaata gccaccagca
    14161 acggagcaag caggccacta aagaaaaaga taatgccaaa tccggcaact aaaataacca
    14221 gcaaggcaat cgcttccggg tcgctaaaac ggcggcgata ccattgcatc aacatttcga
    14281 gcataaaaac cttccctgaa cctcaagagc gggattgcga tccgcaattg tatcgaaatg
    14341 tcacaaaaaa gacttcgctt tttatgacgg attcaggaaa ctgaaaagtc atttgagtgg
    14401 gctaatcttc gccgttacac tcaaaggcgg cgcggtggga acgatatttc acagtatcgg
    14461 tcaaatgact atcttcagaa atacaggata gaggttatgt tcaggcagtt gaaaaaaaac
    14521 ctggttgcaa ccctcattgc tgctatgacc attggtcagg tagccccggc gtttgccgac
    14581 aacgcagaca ccttgccgga tatgggaacc tccgcaggaa gcacgctttc cattggtcag
    14641 gaaatgcaga tgggcgacta ttatgtccgc cagctacgcg gcagcgcgcc gttaattaat
    14701 gacccgctgt taacgcaata tattaattcg ctggggatgc gtctggtttc gcatgccaat
    14761 tcggttaaga caccgtttca tttttttcta atcaacaacg acgaaattaa cgcctttgct
    14821 ttctttggcg gcaacgtggt gctgcactct gccctgttcc gttattccga taacgaaagt
    14881 caactggctt cagttatggc gcacgaaatc tcccacctca cccaacgtca cctggcgcga
    14941 gcgatggaag atcagcagcg cagcacgccg ctgacctggg tcggcgcgtt aggttctatt
    15001 ttactggcga tggccagtcc gcaggcgggg atggcggcgc tgaccggtac actggcggga
    15061 acgcgtcagg ggatgatcag tttcacccag caaaatgaac aggaagcgga ccgcattggt
    15121 attcaggtgc tgcaacgctc gggattcgat ccgcaggcga tgccaacctt cctcgaaaaa
    15181 ttactcgatc aggcgcgtta ctcctcgcgc ccgccggaaa ttttattgac tcacccgttg
    15241 ccggaaagtc gtctggcaga tgcccgcaac cgtgctaatc agatgcgacc gatggtggtg
    15301 cagtcgtcgg aagatttcta tctggcgaaa gcgcgcacac tggggatgta taattccgga
    15361 cgtaaccagc tcaccagtga tttgctggat gaatgggcga aaggaaacgt tcgtcagcaa
    15421 cgagcggcgc aatatggtcg tgctttacag gcgatggaag ccaataaata cgacgaggcg
    15481 cgtaaaacgc tgcaaccgtt actggcggca gaacctggca acgcatggta tctcgatctg
    15541 gctaccgata tcgatcttgg gcaaaacaaa gccaatgagg caatcaatcg cctgaaaaat
    15601 gcccgtgatt tgcgcaccaa tccggtgttg cagctcaacc tggcaaacgc ttatctgcaa
    15661 ggcggtcaac cacaagaagc ggccaatatt ctcaatcgct acacctttaa taataaagat
    15721 gacagcaacg gctgggattt actggcacag gcggaagccg cgctaaataa ccgcgatcag
    15781 gagctggctg cgcgagcaga aggttatgcg ctcgccggac gactcgatca ggccatttcg
    15841 ctgttgagta gcgccagttc gcaggtgaaa ttaggcagcc tgcaacaagc gcgttacgat
    15901 gcgcgcatcg accagttgcg ccagctgcag gaacgcttta agccttatac caagatgtaa
    15961 ttcgcattaa aggaagaaca atgaccaaac aggtaaaaat ctaccataac ccacgctgtt
    16021 caaagagccg ggaaacgctg aacctgctga aagaaaacgg cgtggagcca gaagtggtac
    16081 tctaccttga gacacccgcc gatgcggcaa cgctgcgcga tttgctgaaa atgctgggga
    16141 tgaatagcgc ccgtgaattg atgcgccaga aagaggatct ttataaagaa ctgaacctgg
    16201 ctgacagttc gcttagcgaa gaggcgctga ttcaggcaat ggtggataat ccgaagctga
    16261 tggagcgccc gattgtggtc gcgaatggca aagcgcggat tggtcgtcca ccggagcagg
    16321 tactggaaat cgtgggttaa gatgtgctcc atcgctagtt gaagcacatt gccggatgcg
    16381 acgcttgcca tgtcttatcc ggcctacgaa tactatggat taacttcgcg tagttcggat
    16441 aaggcgttcg cgccgcatcc gacaataaac accttatcta caacttcaga atttctttca
    16501 caaacggaat ggtcagctta cgttgcgcgg taatcgacgc acgatccaac tgatccaacg
    16561 tcataaatag cgtgcgcatt tctctgtcga gccgcttcag caagaaacgc cccacatctt
    16621 ccggcagttc aaaaccacgc aaacgcgcgc gtaactgtag cgcctgcaac ttatcttcat
    16681 cagaaagtgg ctgcaatttg tagatctgcc cccagtcgag tcgcgacgcg agatccggta
    16741 atcccagatt caactgccgc ggtggacgat cgccggtgat caacaaccgt gttttgcccg
    16801 attccagaat tcgattgtag agatcgaaaa tcgccatctc ccacaactca tcgcctgcaa
    16861 tacactcaat gttgtcgata caaaccagcg acaaatgctc cataccgtcg agcacttccg
    16921 gaacaaacca agtgcgttta tccagcggga catagcccac cgcatcgcca cgctgcgaca
    16981 attccgcgca agccgcgtgc agcagatggc tgcgccccgc gccttcgcgt gcccagagat
    17041 agatgtaacc gctatgttcc tgacgcagca cgttttgcag cgcggccagt aaagaggagt
    17101 tatcccccgg ccagaaactt gcaaaggttt cgtcgtcagg aagataaagt ggcaaagaga
    17161 gctgtgccgg tgtgttcaga gatacctcaa ccaggatttc acaaaatcgc gagaagttta
    17221 ccacagaatc ccatgatgtt tgaaccgggc agcaacactg cccggtcggt acattatttg
    17281 tctgttatat ccgcctcttc tgcgtccagc accacttctt cagggcgcag cacactgatc
    17341 agtttgaaaa tcaaacttaa gccgataccg acgatggtcg ccagcgccat accttttaac
    17401 tccgccgcgc cgatgtttac cttcgcgcca ctgacgccga tgatcaaaat cacggaagtc
    17461 aggatcaggt tctgtgcttt gttgtaatcc actttcgatt cgatcaaaac acgaataccg
    17521 gaagcaccga tgacaccata aagcagcagc gaaacgccgc ccatcaccgg caatgggatc
    17581 atctggatag cggcagccag tttaccgacg caggaaagca ggatagcgaa aatcgccgcc
    17641 ccgccgataa cccaggtact gtaaacacgg gtgatcgcca tcacgccaat gttttctccg
    17701 taagtggtat ttggcgtgga gccaaagaag ccggagatca cggtcgacag gccgttagca
    17761 aacatcgaac ggtgcagacc tggatcgcgc agcagatctt ttttgacgat attagccgtt
    17821 actaccaggt gccctacgtg ttcggcaata accactaacg ccgctggcag aatagtcaga
    17881 atggcaaacc actcgaagcg cggcgtatag agggttggca gcgcaaacca gtgagcatta
    17941 ataatcggcg tggtatcgac aattcccatt gcgaaagaga gcgcgtagcc caccagcacg
    18001 ccaattaaaa tcgggataat tgccaggaaa ccacgaaaca gcacggaacc taaaaccgtg
    18061 accgccaggg tggtaataga gatgatgatg gttttggagt ctggcgtttg cccttcagcc
    18121 gggagtaaac ccgccatacc ggcagctacg cccgccagct ccagaccgat gacggcaacg
    18181 attgcgccca ttgccgcagg tggaaacagc acgtccagcc agccggtccc cgctttcttc
    18241 acgataaaag aaaccaggca gaacagcacg ccgcacataa taaagccgcc cagcgcgact
    18301 tcatacccta acggcaacag taacaatacc ggtgaaataa aggcaaagct ggaaccaaga
    18361 taagccggaa ttttcccttt acagatgaag agatacagca gcgttccaat accgttaaat
    18421 aacagtacag tcgccgggtt aatatgaaat aagacgggca ccaggacggt tgcaccaaac
    18481 atggcgaaca aatgttgcaa actaagcggg attgtctgta aaagtggcgg tctttcactc
    18541 accccgatag cacggcgcgt catagtatta tcctctgtat tatgtgttat aggcgcttta
    18601 ctcaaaaaaa agccgactct taaagtcggc tttaattatt tttattcttt atttcgtacc
    18661 aaagattttg tcaccggcat cgccgaggcc cggaataatg tatccgtgct cgttcagtcc
    18721 ctgatcaatc gatgcggtat acagttcgac gtccgggtgc gctttttcca gcgcagcgat
    18781 accttctggc gcagcgacca gcaccagaac tttgatgctg ctgcagcccg cttttttcag
    18841 caggtcgatg gtcgcgataa cggaaccacc ggttgccagc attgggtcaa cgatcagcgc
    18901 catacgctca tcgatgttag aaaccagttt ctggaagtac ggtaccggct ccagcgtttc
    18961 ttcattacgg tacataccga caacgctgat gcgcgcgctc ggaacatttt ccagcacacc
    19021 gtccatcata ccaagacccg cacgcagaat tggcacaacg gtaattttct tacctttgat
    19081 ctggtcgatt tctaccgggc cgttccagcc ttcgatagtt actttttccg tttcgaggtc
    19141 ggcggtcgct tcgtaagtca gcaggctacc cacttcggaa gcgagttcgc gaaagcgctt
    19201 ggtgctgata tcttgctcac gcatcagtcc cagcttgtgt ttgacgagtg ggtgtttgac
    19261 ttccacgatc ttcatactct ttctcctttg aggggcagcc acaaaaaaat cgacggatta
    19321 tacctccttt cttcaaggcg gcaatattct tttcgttgac tttagtcaaa atgataacga
    19381 tttgagataa agttatttta tattcaaatg gttatgaaag aagatcattc catccgaaaa
    19441 ctaacctttg ccctggcaca agtcttcttt cgccgcgcgt ctggggaaaa gacgtgcaaa
    19501 aaggttgtgc aaagcagcct cgcaaacgtt tgctttccct gttagaattg cgccgaattt
    19561 tatttttcta ccgcaagtaa cgcgtgggga cccaagcagt gaccgataaa acctctctta
    19621 gctacaaaga tgccggtgtt gatattgacg cgggtaatgc tctggttgga agaatcaaag
    19681 gcgtagtgaa gaaaacgcgt cgtccggaag tgatgggcgg tctgggcggc ttcggtgcgc
    19741 tgtgtgcatt gccgcaaaaa tatcgtgaac ccgtgctggt ttccggcact gacggcgtag
    19801 gtaccaagct gcgtctggca atggacttaa aacgtcacga caccattggt attgatctgg
    19861 tcgccatgtg cgttaatgac ctggtggtgc aaggtgcaga accgctgttt ttcctcgact
    19921 attacgcaac cggaaaactg gatgttgata ccgcttcagc ggtgatcagc ggcatcgcgg
    19981 aaggttgtct gcaatcgggc tgttcactgg tgggtggcga aacggcagaa atgccgggga
    20041 tgtatcacgg tgaagattac gatgtcgcgg gtttctgcgt gggcgtggta gaaaaatcag
    20101 aaatcatcga cggctctaaa gtcagcgacg gcgatgttct gattgcactc ggttccagcg
    20161 gtccgcactc gaacggctat tcgctggtgc gcaaaattct tgaagtcagc ggttgtgatc
    20221 cgcaaaccac cgaacttgat ggtaagccat tagccgatca tctgctggca ccgacccgca
    20281 tttacgtgaa gtcagtgctg gagttgattg aaaaggtcga tgtgcatgcc attgcgcacc
    20341 tgaccggcgg cggcttctgg gaaaacattc cacgcgtatt gccagataat actcaggcag
    20401 tgattgatga atcttcctgg cagtggccgg aagtgttcaa ctggctgcaa acggcaggta
    20461 acgttgagcg ccatgaaatg tatcgcacct tcaactgcgg cgtcgggatg attattgccc
    20521 tgcctgctcc ggaagtggac aaagccctcg ccctgctcaa tgccaacggt gaaaacgcgt
    20581 ggaaaatcgg tatcatcaaa gcctctgatt ccgaacaacg cgtggttatc gaataatgaa
    20641 tattgtggtg cttatttccg gtaacggaag taatttacag gcaattattg acgcctgtaa
    20701 aaccaacaaa attaaaggca ccgtacgggc agttttcagc aataaggccg acgcgttcgg
    20761 ccttgaacgc gcccgccagg cgggtattgc aacgcatacg ctcatcgcca gcgcgtttga
    20821 cagtcgtgaa gcctatgacc gggagttgat tcatgaaatc gacatgtacg cacccgatgt
    20881 ggtcgtgctg gctggtttta tgcgcattct cagcccggcg tttgtctccc actatgccgg
    20941 gcgtttgctg aacattcacc cttctctgct gccgaaatat cccggattac acacccatcg
    21001 tcaggcgctg gaaaatggcg atgaagagca cggtacatcg gtgcatttcg tcaccgatga
    21061 actggacggt ggcccggtta ttttacaggc gaaagtcccg gtatttgctg gtgatacgga
    21121 agatgacgtc accgcccgcg tgcaaaccca ggaacacgct atttatccac tggtgattag
    21181 ctggtttgcc gatggtcgtc tgaaaataca cgaaaacgcc gcgtggctgg atggtcaacg
    21241 tctgccgccg cagggctacg ctgccgacga gtaatgcccc cgtagttaaa gcgccagctc
    21301 tgccgctggc gtttttcaat tcacctgtaa atcgcaagct ccagcagttt ttttccccct
    21361 tttctggcat agttggacat ctgccaatat tgctcgccat aatatccagg cagtgtcccg
    21421 tgaataaaac ggagtaaaag tggtaatggg tcaggaaaag ctatacatcg aaaaagagct
    21481 cagttggtta tcgttcaatg aacgcgtgct tcaggaagcg gcggacaaat ctaacccgct
    21541 gattgaaagg atgcgtttcc tggggatcta ttccaataac cttgatgagt tctataaagt
    21601 ccgcttcgct gaactgaagc gacgcatcat tattagcgaa gaacaaggct ccaactctca
    21661 ttcccgccat ttactgggca aaattcagtc ccgggtgctg aaagccgatc aggaattcga
    21721 cggcctctac aacgagctat tgctggagat ggcgcgcaac cagatcttcc tgattaatga
    21781 acgccagctc tccgtcaatc aacaaaactg gcttcgtcat tattttaagc agtatctgcg
    21841 tcagcacatt acgccgattt taatcaatcc tgacactgac ttagtgaagt tcctgaagga
    21901 tgattacacc tatctggcgg tggaaattat ccgtggagac accatccgtt acgcgctgct
    21961 ggagatccca tcagataaag tgccgcgctt tgtgaattta ccgccagaag cgccgcgtcg
    22021 acgcaagccg atgattcttc tggataacat tctgcgttac tgccttgatg atattttcaa
    22081 aggcttcttt gattatgacg cgctgaatgc ctattcaatg aagatgaccc gcgatgccga
    22141 atacgattta gtgcatgaga tggaagccag cctgatggag ttgatgtctt ccagtctcaa
    22201 gcagcgttta actgctgagc cggtgcgttt tgtttatcag cgcgatatgc ccaatgcgct
    22261 ggttgaagtg ttacgcgaaa aactgactat ttcccgctac gactccatcg tccccggcgg
    22321 tcgttatcat aattttaaag actttattaa tttccccaat gtcggcaaag ccaatctggt
    22381 gaacaaacca ctgccgcgtt tacgccacat ttggtttgat aaagcccagt tccgcaatgg
    22441 ttttgatgcc attcgcgaac gcgatgtgtt gctctattat ccttatcaca cctttgagca
    22501 tgtgctggaa ctgctgcgtc aggcttcgtt cgacccgagc gtactggcga ttaaaattaa
    22561 catttaccgc gtggcgaaag attcacgcat catcgactcg atgatccacg ccgcacataa
    22621 cggtaagaaa gtcactgtgg tggttgagtt acaggcgcgt ttcgacgaag aagccaacat
    22681 tcactgggcg aagcgcctga ccgaagcagg cgtgcacgtt atcttctctg cgccggggct
    22741 gaaaattcac gccaaactgt tcctgatttc acgtaaagaa aacggtgaag tggtccgtta
    22801 cgcacacatc gggaccggga actttaacga aaaaaccgcg cgtctttata ctgactattc
    22861 gttgctgacc gcagatgcgc gtatcaccaa cgaagtacgg cgggtattta actttattga
    22921 aaacccatac cgcccagtga catttgatta tttaatggtg tcaccgcaaa actctcgccg
    22981 tctgttatat gaaatggtag accgcgaaat cgccaacgcg cagcaagggc tgcccagtgg
    23041 tatcaccctg aagctaaata accttgtcga taaaggcctg gttgatcgtc tgtatgcggc
    23101 ctccagctcc ggcgtaccgg ttaatctgct ggttcgcgga atgtgttcgc tgatccccaa
    23161 tccggaaggc attagcgaca acattcgtgc catcagtatt gttgaccgtt accttgaaca
    23221 tgaccgggtt tatatttttg aaaatggcgg cgataaaaag gtctaccttt cttccgccga
    23281 ctggatgacg cgcaatattg attatcgtat tgaagtggcg acaccgctgc tcgatccgca
    23341 cctgaagcag cgggtgctgg acatcatcga catattgttc agcgatacgg tcaaagcacg
    23401 ttatatcgat aaagaactca gtaatcgcta cgttccccgc ggcaatcgcc gcaaagtacg
    23461 ggcgcagttg gcgatttacg actacatcaa atcactcgaa caacctgaat aaccctatgc
    23521 caatacacga taaatcccct cgtccgcagg agtttgctgc ggtcgatctt ggttcaaaca
    23581 gttttcacat ggtcatagcc cgtgtggtag atggtgccat gcagattatt ggccgcctga
    23641 aacagcgagt gcatctggcg gacggcctgg ggccagataa tatgttgagt gaagaggcaa
    23701 tgacgcgcgg tttgaactgt ctgtcgctgt ttgccgaacg gctacaaggg ttttctcctg
    23761 ccagcgtctg tatagtcggc acccatacgc tgcgtcaggc gctgaacgcc actgactttc
    23821 tgaaacgcgt ggaaaaggtc attccctacc cgattgaaat tatttccggt aatgaagaag
    23881 cccgtctgat ttttatgggc gtggaacata cccaaccgga gaaaggtcgc aaactggtta
    23941 ttgatattgg cggcggatct acggaactgg tgattggtga aaatttcgaa ccgattctcg
    24001 ttgaaagccg ccggatgggt tgtgtcagct ttgcccagct ttacttccct ggcggggtca
    24061 tcaataaaga gaattttcag cgcgctcgta tggcggcagc acaaaaactg gaaactttaa
    24121 cctggcaatt ccgtattcag ggctggaacg tggcaatggg cgcttccggt accataaaag
    24181 ccgcccatga agtgttaatg gaaatgggcg agaaagacgg gataattacc ccggaacgtc
    24241 tggaaaaact ggtaaaagaa gttttacgtc accgtaattt cgcatcgctg agtttaccgg
    24301 gtctttccga agagcggaaa acagtcttcg ttcctggact ggcgatttta tgcggtgtgt
    24361 ttgatgcttt agccatccgt gaactgcgcc tttctgacgg ggcgttacgc gaaggcgtac
    24421 tgtacgaaat ggaaggacgt ttccgtcatc aggatgtgcg tagtcgcacc gccagcagcc
    24481 tcgccaacca gtatcacatc gacagcgaac aggcccgacg ggtgctggat accactatgc
    24541 aaatgtacga acagtggcgg gaacagcaac cgaagctggc gcatccgcaa ctggaggcgc
    24601 tactgcgatg ggccgccatg ctgcatgagg tcgggttgaa tatcaaccac agcggtttgc
    24661 atcgccactc cgcttatatt ctgcaaaaca gtgacttgcc gggttttaat caggaacagc
    24721 agctgatgat ggcgacactg gtgcgctatc accgcaaagc gattaaactc gacgatctgc
    24781 cgcgctttac cttgtttaag aagaaacagt tcctgccact gattcagcta ttgcgccttg
    24841 gcgtattact caacaatcaa cgtcaggcaa ccaccacacc gccaacattg acactgatta
    24901 ctgatgacag tcactggaca ctgcgtttcc cgcatgactg gtttagtcag aatgcgttgg
    24961 tactgcttga tctggaaaag gagcaagaat actgggaagg cgtggctggc tggcggttga
    25021 aaattgaaga agaaagtaca cctgaaatcg ccgcttaatg ctttgcgggc cgacgagaaa
    25081 tgtcggcccg cattattcag gcactttcgc gaatgggtt
//
LOCUS       DADLFA010000043        25002 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000043 DADLFA010000000
VERSION     DADLFA010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25002
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.043"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..808
                     /locus_tag="JQH65_001497"
     CDS             <1..808
                     /locus_tag="JQH65_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAY6138961.1"
                     /translation="PRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTG
                     GRLLMFGVGVIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVIN
                     LTFTVLAIMTVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAF
                     AMSWGPVCWVLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHN
                     GFSYWIYGCMGVLAALFMWKFAPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(852..1250)
                     /gene="psiE"
                     /locus_tag="JQH65_001498"
     CDS             complement(852..1250)
                     /gene="psiE"
                     /locus_tag="JQH65_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HAY6138962.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLIIVDHK
                     SPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            1520..1798
                     /locus_tag="JQH65_001499"
     CDS             1520..1798
                     /locus_tag="JQH65_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6138963.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(1845..3941)
                     /locus_tag="JQH65_001500"
     CDS             complement(1845..3941)
                     /locus_tag="JQH65_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HAY6138964.1"
                     /translation="MKKRHLLSLLALGISMACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNVGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     MADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVLKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(3941..4678)
                     /locus_tag="JQH65_001501"
                     /pseudo
     CDS             complement(3941..4678)
                     /locus_tag="JQH65_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
     gene            complement(4675..5313)
                     /locus_tag="JQH65_001502"
     CDS             complement(4675..5313)
                     /locus_tag="JQH65_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HAY6138965.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKILLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(5427..5669)
                     /locus_tag="JQH65_001503"
     CDS             complement(5427..5669)
                     /locus_tag="JQH65_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6138966.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(6131..7780)
                     /gene="pgi"
                     /locus_tag="JQH65_001504"
     CDS             complement(6131..7780)
                     /gene="pgi"
                     /locus_tag="JQH65_001504"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAY6138967.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            8305..9654
                     /gene="lysC"
                     /locus_tag="JQH65_001505"
     CDS             8305..9654
                     /gene="lysC"
                     /locus_tag="JQH65_001505"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HAY6138968.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNDGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKHIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            9935..10177
                     /locus_tag="JQH65_001506"
     CDS             9935..10177
                     /locus_tag="JQH65_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HAY6138969.1"
                     /translation="MTEREQRELKTLLDRARIAHGRVLTNSETNSIKKEYIDKLMVER
                     EAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(10310..11182)
                     /gene="rluF"
                     /locus_tag="JQH65_001507"
     CDS             complement(10310..11182)
                     /gene="rluF"
                     /locus_tag="JQH65_001507"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HAY6138970.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            11530..12477
                     /locus_tag="JQH65_001508"
     CDS             11530..12477
                     /locus_tag="JQH65_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HAY6138971.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIVGALRGKYINCLVTNSSTAELLLK"
     gene            12547..13353
                     /locus_tag="JQH65_001509"
     CDS             12547..13353
                     /locus_tag="JQH65_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY6138972.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALVWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            13363..13770
                     /locus_tag="JQH65_001510"
     CDS             13363..13770
                     /locus_tag="JQH65_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HAY6138973.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDARVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            13770..14264
                     /locus_tag="JQH65_001511"
     CDS             13770..14264
                     /locus_tag="JQH65_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HAY6138974.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIDTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            14330..15127
                     /locus_tag="JQH65_001512"
     CDS             14330..15127
                     /locus_tag="JQH65_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAY6138975.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSAFGVQALRVAIPALIVSL
                     FVSADMVSNMLSAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            15138..15980
                     /locus_tag="JQH65_001513"
     CDS             15138..15980
                     /locus_tag="JQH65_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000437910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAY6138976.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITTALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VTMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLSRF
                     FAPA"
     gene            16014..17243
                     /locus_tag="JQH65_001514"
     CDS             16014..17243
                     /locus_tag="JQH65_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAY6138977.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRMLILGGTGPMGLLAIDYALHGPVNPSQLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLTHAEAISHD"
     gene            17626..18315
                     /gene="pepE"
                     /locus_tag="JQH65_001515"
     CDS             17626..18315
                     /gene="pepE"
                     /locus_tag="JQH65_001515"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HAY6138978.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(18406..20037)
                     /locus_tag="JQH65_001516"
     CDS             complement(18406..20037)
                     /locus_tag="JQH65_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HAY6138979.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTAALTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQSDEDEGRDEY"
     gene            complement(20257..23940)
                     /gene="metH"
                     /locus_tag="JQH65_001517"
     CDS             complement(20257..23940)
                     /gene="metH"
                     /locus_tag="JQH65_001517"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAY6138980.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYCESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEAQDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTDNAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNCIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            24140..24964
                     /gene="iclR"
                     /locus_tag="JQH65_001518"
     CDS             24140..24964
                     /gene="iclR"
                     /locus_tag="JQH65_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HAY6138981.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMEVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         5878 a         6610 c         6535 g         5979 t
ORIGIN      
        1 tcctcgctgg ctgatgtcac gcggcaagca agaacaggcg gaaagtatcc tgcgcaaaat
       61 tatgggcaac acgcttgcaa ctcaggcagt acaggaaatt aaacactccc tggatcatgg
      121 ccgcaaaacc ggtggtcgtc tgctgatgtt tggcgtgggc gtgattgtca tcggcgttat
      181 gctctccatc ttccagcaat ttgtcggcat caatgtggtg ctgtactacg cgccggaagt
      241 gttcaaaacg ctgggggcca gcacggatat cgcgctgttg cagaccatta ttgtcggagt
      301 tatcaacctc accttcaccg ttctggcaat tatgacggtg gataaatttg gtcgtaagcc
      361 actgcaaatt atcggcgcac tcggaatggc aatcggtatg tttagcctcg gtaccgcgtt
      421 ttacactcag gcaccgggta ttgtggcgct actgtcgatg ctgttctatg ttgctgcctt
      481 tgccatgtcc tggggtccgg tatgctgggt actgctgtcg gaaatcttcc cgaatgctat
      541 tcgtggtaag gcgctggcaa tcgcggtggc ggcccagtgg ctggcgaact acttcgtctc
      601 ctggaccttc ccgatgatgg acaaaaactc ctggctggtg gcccatttcc acaacggttt
      661 ctcctactgg atttacgggt gtatgggcgt tctggcagca ctatttatgt ggaaatttgc
      721 cccggaaacc aaaggtaaaa cccttgagga gctggaagcc ctctgggaac cggaaacgaa
      781 gaaaacacaa caaactgcta cgctgtaatc ttcctgtcca gcacgccgcg ccagttcggc
      841 gtgctgtctt tttactcccg cttcagccgt ttcgaattac acagccacag agtgatcacc
      901 agtaacagga tcgcagccga gtagatcaac acatccagtg gcgatttatg atcgacgatg
      961 atcaagcgca caatggcggt gatcccaata tagacaaagt aacgtagcgg gaagtgaaaa
     1021 ccggactgaa agtacttcac aatcagcgcg ataaattcga aatagagaaa gtaaaccacc
     1081 agctcatatt tgctggtttg ttctggcgcg aacagcacat ctgccagatg caccgtttct
     1141 ttgccgagga agacaaccaa aatcaggccg aggcacagca ggccaagatt gagtacggtc
     1201 tgcaaaatgg tggagataaa ctccacgcgc ggacgagaga gtgacgtcat agcagtacct
     1261 ccttcacagt ggcgagcttc ctgtataacg caaaaatgtg acggagatct atattttttg
     1321 tgtatgtttt gttcaacctg gatttggagc gcgcctccac caaagccgga tatacctacc
     1381 cgtaggccgg ataagccgcg cctggcggcg catccggcaa gacaaaccgc acccaagccg
     1441 gtaattcaaa aatcacccat tcaggtgatt cccagccacc agcaatgcgc gaaaaatcga
     1501 caatgttcct ctggaacaaa tgaaacgtaa cctgataaag gtggtaaaaa tgaagcctta
     1561 ttttgctgct ttgatgttat cagtctctgt tcttcctgct tatgctggtc cgctcggtac
     1621 tgctgataaa gccgacctac cgcaaagcaa tgtctccagc ccaatgatgg cccagtcgct
     1681 caggcaacca gacctgcaac cgatatcgac ggacaggaag acagaatgtt ttcgccttta
     1741 cacaccagat cgcaaaccgg gggtgaactg cgttcctgat ggctcgacag gccattaaga
     1801 atgaaggtgc cagtgagtgc gaaaactggc acctttccca tgacttagcg gaaattgacg
     1861 ctacggtcgc tggtcatgtc atacaactgg aacttacgcc caagttgctg accaccgtca
     1921 cgcgtcagcg gcgtccagcc aatcgccgca cggctgcgtg tcgggccaga cgagaagaga
     1981 tccaacggta ccgagacata cacgcctttg gtgaagtccc cttcgccgta ctcctctttc
     2041 aaaacgttgg tgatcgtggc atagccaccc accaccacgc cgctgtcaaa gcgtttggcg
     2101 atctccagcg tgccgccttt atcccctgcc agatactgcc cgacgctggc tttcaccaac
     2161 acgtcctggg cgaaagatgg cgtccagtag gcggtcagat gtccggtttt cacgctgtag
     2221 tcggtgaatt tcatcatatc ttttgcacta cgccagtcgc gctgtttaac gtagttggca
     2281 tccagaccaa acgcccagtt actgtccagc ggacgataca acacttcggc ccccgcaccg
     2341 ccaaacatgg tttcgagata acctccatag acctgaccgt agaagccgtt gcccagatgc
     2401 tggaagtagt tggcttgcag gttattcaca tagacatcgt tctgcacata ctcgcgcaca
     2461 tgggtacgaa cgcgcggtaa gtgcgagtcc tgcggcggat tggtgtagtt aaacttgtcg
     2521 tagttgttgg caagatttgc aaacaggctg ccggtggtca gcagatggtc cgtcagccac
     2581 aaatctgcca ttcccatcac gcccagctga tacatgtaaa agttttccgg gccaccgacc
     2641 gactggttca gcaccggatc gatatggaaa tcaaagcgtg atttgtcgat ataccagccc
     2701 tgctcggtgg actgcggaac cactggctcg acgcgttttt gcgccagctt cgtttcgtgg
     2761 cccaacggct ctccggcgag atgatttttc aggctggcga catcggtttc cgtcgtcacc
     2821 tgcggcatgt taagacgatt ttccgtaatg cggatcgttt tgatcccatc cggcagatcg
     2881 ttcatcacga tccgattagc acggatgatc ccttcgcgcg aatcacgata tttcacctgc
     2941 tcgccggtga catacagcgt atcgcctttc gcctggatct gcggatcggc aagtccgaca
     3001 ttgtatttca acagcgttaa ctgattcgcc accaccgaat gctgcaaaat ggcatcctgc
     3061 ggctgcggtt gatattgcgg gcgggcgtta tcgttgtaag acgggcgcag atcgttaaag
     3121 ttggtgcgca gcgtgacgcc aaacataaag gtgttgccac gttcatagct aaggttaacg
     3181 tcggcccaat cggtaacgcg ataaatcgca ccgacgttaa acttactttt ttgctccagc
     3241 ttcccggcaa aatcctgctg ataattattg ccttcatact ccagtttcag gcgcagcggt
     3301 tgccagggcg tctggtattc cacgccgcca aacagtgagg caggaccgtg gaacatctgg
     3361 ctaccgtcga tagatcccgc ctgtttgtag ctgttatcgc gatagcaata tttatcactg
     3421 gctgaacaga gcggattttt cacattaccg ctggtgccca gataacccca gcccaggccg
     3481 agcgtaaaat cgaacggccc ccaggctttg ctggcaacaa gatattccgc atcaaacagc
     3541 cccgtaccgc cgatatccct cgcaccaacc gccacttgcg gcagccagta actctcttcc
     3601 cacaaacgca gtttgagatc gaaggcttta tctttatacg tttgatcgcc agagaacgct
     3661 tcgacgctgc tgtactgccg ggtgcgcacg tcggtgtagc gcagcgttgt ttccagccac
     3721 gggaagagtt gcactgaagc tgagtaataa cggtactgat cgttatcgcg atagttcagg
     3781 ctcaactccc cttcccgcgc catgcgtgcg gtgggcgttt gtaataatcc tacgccaccg
     3841 aaatccgact gcgacgggcc aatgggcgca ggatatgttt cgccgtagca agccatgcta
     3901 atgcccagcg ccagtaagct aagcagatgt ctttttttca ttattggggt atccgctgcg
     3961 tcagagtctg aagaatgtcg gcgttaaggg catcaggcgt ctcactccag acggagtccg
     4021 cgaggccaac ataaataatg ctgccgggca tcggctctac gtgacgcttg ttccagtaag
     4081 ccaccggcgc tttttgcgtg cgtccgtccg ggtaaaccac ccacgcgtag ctgcgatccg
     4141 caccgctaag caggttctgc ccggagagat agctcgccac gtcgcgacct ggagtgaatg
     4201 gctgcttgcc aggacggctg ataagcccga acaacgtgac cgtggacggc ggtggtccga
     4261 cccacagcgt gtagttgccc tgcaacggtg ggttaccgcg ttcggcaacg cgcacgatat
     4321 cgggatcaag attgattttt tgcctgcccg tcactttcaa cgcctgaatt tgctggcgta
     4381 aggcgttaat ggcggcggca tcgtcggcgc tggagtctgc ggcctgttca gccagccgtg
     4441 tcagcaacgc ctgctgctga cgcaatgctg ccgccgttgc cagctcttcg ctaatcaccg
     4501 caccgggcca ccagttgttt gccagccgcg gttagcctac cagatcgatt aaatgctctg
     4561 cgcctgttag tgttttggcc tcactgctgc cattgctgaa caccttgacg gttcctgccg
     4621 cgaagaccga tgacgctccc acactcaaaa gcaacgcgac aaaagtttgt ttaatcattg
     4681 ttttgccgcc ttgatgagag tggttttcac cgggaaataa tccgcgccga gatactgttc
     4741 cgactggcga atttgccctt cgctgtcgat ccagtagcgg ttatgccagc gcgcctggtc
     4801 ggtggagact tcttcgtcca gcacgcgaac cagggtttca tcgctgccga ctttgacggt
     4861 atcggtgcca tcccatttga agactgagcg tgcggtggcg tagcgtacct gctggtactc
     4921 ggtccagccc atcgtgcgcg tccatgttgc accatcaaca atttgtgcgg gtttaatcag
     4981 cgggtcggcg gcaaggttat tcacttcaat caggttgtcg ccgccaagca aaatcttcac
     5041 cagacgacca tgttgtgtga caagagtggc ttgatcctga gtgacccatt tttgttgtcc
     5101 gtcttcagcg aaggccagca ccacaaacaa ttgcggcccg ccattaagct gcatgtactg
     5161 gctggcgtag ggcatatttt gaatatcatc gtccgtcagc tgtacgcctg gcgtgccgaa
     5221 cagactgtcc cacagtgaat tgcccagctc tttagtcgtg gctgaacagg cctgtaatag
     5281 caggcagata agaatgagtg caggtcgctt cacgactctt ctccagaggg gcagaataac
     5341 cacaccggag tgtggttatg gtaaatccct tcatacttca agccacagat gcaatgactc
     5401 aaattatttt ggggatacat actttattac tgggtactgg tggtcgtggt tgtggtggtc
     5461 ccggtattag aaccatcacc gccaccggtt gccgcgagcg cgacacccac cgccgagctt
     5521 acggtgctga cgctggtcgc ggaggaacta cccgccgaaa ccgacgttgc tgccgaccct
     5581 gccgcttcgc ccacctgtac aggtgcagcc cacgcagaag tcgccgcaag cgcagatatg
     5641 gcaaaaatgc catacagaac ttttttcata acaatttccc ttcattgaat gaatggagat
     5701 ttacccaaaa acatttcggg cgggatcgag tatacacaac taaagcatgc gaattacggc
     5761 gcggggaatt agcgatggcg ttttaggata attggaatga atatcaaata taagcttcaa
     5821 cttatattta aataacaata aaaatcataa acttaaaata aaaacaaaag taattaatct
     5881 gcgtattctc cgaaataaac aacgattaaa aatctatttt aggattaacc tcacggtatg
     5941 atttccgtta aatcacagac aaggggattt atctgataaa acaatgccga cttattgtcg
     6001 gcattgtttt taaggttatt taactcctca tactatgcat taatgtcgat attggcatca
     6061 ggctccggtt gccggatgcg gcgtaaacgc cttatccggc ctacatatcg gcatcaggca
     6121 cgcatgatga ttaaccgcgc cacgctttat agcggttaat cagaccattg gtcgagctat
     6181 cgtggctgct gatttctttg tcatctttca gctctggcag aatacggttc gccagttgtt
     6241 tacccaactc cacgccccac tggtcgaagg taaagatgtt caggatcacg ccctgagtaa
     6301 agattttgtg ctcatacagc gcaatcaacg cccccaggct gaacggagtg atttcgcgca
     6361 gcaggatgga gttggtcggg cggttacctt cgaacacttt gaacggcacc acgtagtcaa
     6421 gcgttgccgg atctttaccc tgatcgcgat attcctgctc aaccacttcg cgggatttac
     6481 caaacgccag cgcttcggtc tgggcgaaga agttagacag cagtttctgg tggtgatcag
     6541 agagcgggtt atgggtgata gccggggcga tgaaatcgca cggtaccatt ttggttccct
     6601 ggtggatcag ctggtagaac gcgtgctgac cgttagtgcc tggttcaccc cagataatcg
     6661 ggccagtctg gtaatccaca acgttaccgt tacggtcaac atacttaccg ttggactcca
     6721 tattgccctg ctggaagtac gccgcgaaac ggtgcatata ctggtcatac ggcagaatcg
     6781 cttcagtttc cgcaccaaag aaattgttgt accagatgcc aatcagcgcc agcaggacag
     6841 gcaggttttt ctcggcaggc gtggtggaga aatgcttgtc catcgcgtgt gcgccagaaa
     6901 gcagttcaac gaagttatca aagccgatgg agagaacaat cgacaggcca atcgctgacc
     6961 acaaagagta acggccgccg acccagtccc agaactcgaa catgttggca gtatcaatac
     7021 caaactcgcc aacggctttg gcattggtgg aaagcgccgc aaagtgtttt gcaacgtgtt
     7081 tttcatcacc tgccgctttc aggaaccagt cacgcgcgct atgggcgttg gtcatagttt
     7141 cctgagtggt gaaggtttta gatgctacca agaacagcgt ggtttccggg tttacttttt
     7201 tcagcacttc agcgatgtga gtcccatcga cgttagaaac aaagtgcatg ttcaggtgat
     7261 ttttgtacgg acgcagtgct tcggtcacca tgtacgggcc gaggtcagaa ccaccgatcc
     7321 cgatgttcac tacgtcagtg attgctttgc cggtataacc cttccactca ccggagataa
     7381 tcgcttccga gaaggttttc atcttctcca gcaccgcgtt gacttccggc attacgtctt
     7441 tgccatcaac cagaatcggg gtattgctac ggttacgcag cgctacgtgc agcacggcgc
     7501 ggttttcagt gcggttgatc ttctcgccag agaacatcga cttaatcgcg cccgccagat
     7561 cgcactcttt cgccagatcc tgtaatttcg ccagcgtctc ttcagtgatg cggtttttgg
     7621 agtaatccac cagcatctga tcgtcgaatg ttgcggagaa cttagaaaaa cgatcgccgt
     7681 ctttagcaaa aagatcggcg atcgtaacgt ctttcatttc atcgaagtgt ttctgtagtg
     7741 cctgccaggc agcggtctgc gttggattga tgtttttcat tagcaatact cttctgattt
     7801 tgagaattgt gactttggaa gattgtagcg ccagtcacag aaaaatgtga tggttttagt
     7861 gccgttagcg taatgttgag tgtaaaccct tagcgcagtg aagcatttat tagctgaact
     7921 actgaccgcc aggagtggat gaaaaatccg catgaccccg tcgttgacaa ccgccccgct
     7981 caccctttat ttataaatgt actacctgcg ctagcgcagg ccagaagagg cgcgttgccc
     8041 aagtaacggt gttggaggag ccagtcctgt gataacacct gagggggtgc atcgccgagg
     8101 tgattgaacg gctggccacg ttcatcatcg gctacagggg ctgaatcccc tgggttgtca
     8161 ccagaagcgt tcgcagtcgg gcgtttcgca agtggtggag cacttctggg tgaaaatagt
     8221 agcgaagtat cgctctgcgc ccacccgtct tccgctcttc ccttgtgcca aggctgaaaa
     8281 tggatcccct gacacgaggt agttatgtct gaaattgttg tctccaaatt tggcggtacc
     8341 agcgtagctg attttgacgc catgaaccgc agcgctgata ttgtgctttc tgatgccaac
     8401 gtgcgtttag tagtcctctc ggcttctgct ggtatcacta atctgctggt cgctttagcg
     8461 gaagggctgg aacctggcga gcgattcgaa aaactcgacg ctattcgcaa catccagttt
     8521 gccattctgg aacgtctgcg ttacccgaac gttatccgtg aagagattga acgtctgctg
     8581 gagaacatta ctgttctggc agaagcggcg gcgctggcaa cgtctccggc gctgacagat
     8641 gagctggtca gccacggcga gctgatgtcg accctgctgt ttgtcgaaat cctgcgcgaa
     8701 cgcgatgttc aggcacagtg gtttgatgta cgtaaagtga tgcgtaccaa cgaccgattt
     8761 ggtcgtgcag agccagatgt agccgcgctg gcggaactgg ccgcgctgca gctgctccca
     8821 cgcctcaatg acggcttagt gatcacccag ggatttatcg gtagcgaaaa taaaggtcgt
     8881 acaacgacgc ttggccgtgg aggcagcgat tatacggcag ccttgctggc ggaggcttta
     8941 cacgcatctc gtgttgatat ctggaccgac gtcccgggca tctacaccac cgatccacgc
     9001 gtagtttccg cagcaaaaca cattgatgaa atcgcgtttg ccgaagcggc agagatggca
     9061 acttttggtg caaaagtact gcatccggca acgttgctac ccgcagtacg cagcgatatc
     9121 ccggtctttg tcggctccag caaagaccca cgcgcaggtg gtacgctggt gtgcaataaa
     9181 actgaaaatc ctccgctgtt ccgcgcgctg gcgcttcgtc gcaatcagac tctgctcact
     9241 ttgcacagcc tgaatatgct gcattctcgc ggcttcctcg cggaagtttt cggcatcctc
     9301 gcgcggcata atatttcggt agacttaatc accacgtcag aagtgagcgt ggcattaacc
     9361 cttgatacca ccggttcaac ctccactggc gatacgttgc tgacgcaatc tctgctgatg
     9421 gagctttccg cactgtgccg ggtggaagta gaagaaggtc tggcgctggt cgcgttgatt
     9481 ggcaatgacc tgtcaaaagc ctgcggcgtt ggcaaagagg tattcggcgt actggaaccg
     9541 ttcaacattc gcatgatttg ttacggcgca tccagccata acctgtgctt cctggtgccc
     9601 ggcgaagatg ccgagcaggt ggtgcaaaaa ctgcatagta atttgtttga gtaaatactg
     9661 tatggcctgg aagctatatt tcgggccgta ttgattttct tgtcactatg cttatcaata
     9721 aacgagcctg tactctgtta accaacgtct ttatcgagaa taattgcctt taattttttt
     9781 atctgcatct ctaattaatt atcgaaagag ataaatagtt aagagaaggc aaaatgaata
     9841 ttatcagttc tgctcgcaaa cgaattcccg tacactgaac tctctggctc actgagggca
     9901 ccatcatggc actccccgta ttacccaaaa agagatgact gaacgcgagc agcgtgaact
     9961 taaaacactg ctggatcggg cgcggattgc gcatggtcgc gtactgacca actccgaaac
    10021 caacagcatt aagaaagagt acatcgataa attaatggtc gagcgtgagg ctgaagcgaa
    10081 aaaagcccgc caattgaaga aaaagcaggc ttataaaccg gatcctgaag cgtcatttag
    10141 ctggtcagca aatacgtcga cgcgcggcag gcgctaatga acaatcaacg cctgagattt
    10201 tcactcgtct gtgtggacac aatcttcccc tcaccctaac cctctccccg aaggggcgag
    10261 gggactgtcc gagctctttt tggacgtttt catcagtctg aggcgttaat cagcgtccct
    10321 tctttttacg ccccggcgag gtaaaacgct taccgttgga cgccgggcga ccgccttttt
    10381 ccgccgtttt ttccatctta acgactggac gtttgatgcc cgctgttttc ggtttggcct
    10441 tcgctttagg ttttgcctcg gaagaggaat tttcgatgag cttaaagagg tcgattaact
    10501 catcgtcggt taaatcgcgc cactccccca gcggaatgcc gcttaagctg acgttcataa
    10561 tgcgcgtacg ttccagcttt ttcacttcat agccgaaatg ctcgcacatg cgccggatct
    10621 gacggttcag cccctgcacc agggtaatgc ggaagacaaa cggcgcttct tttttgactt
    10681 tgcacttttt cgtcaccgtc ccgaggatcg gcacccccgc ccccatacca cgaataaatt
    10741 catcggtaat cggtttatcg accgtcacca gatactcttt ctcatgatcg ttacccgcac
    10801 ggaggatctt attcaccaga tcgccgtgat tggtgaggaa aatcagcccc tgggagtctt
    10861 tatccaggcg gccaatcggg aacacgcgtt tgctgtggtt aacgaaatcg acaatgttat
    10921 cgcgctcgcc atcttcggtg gtgctgacaa taccgacggg cttgttcagg gcgataagta
    10981 ccaaatcttc ggcttcccga ggctcaatca actgaccgtt tactttcaca atgtcgccgg
    11041 gtttcacctg atcgccaatg gtggctcgct tgccattaag gaacacattg ccttgctcga
    11101 tatagcgatc cgcttcgcgg cgtgagcaaa ttccgctttc gctgatgtat ttatttaaac
    11161 ggactgatga gtcgggcagc ataaattctc ctgtaaaacg cggaatatac cgtacctctt
    11221 agcggggaaa aacagggtta tgcggcaacg tcgaaattaa atgtgatctg ccacgccttt
    11281 tatcacaaaa gtgtctccgg aagtacgatg caaatttgcc tggcaaaata aggataatca
    11341 acatattacg aatcgctgca cacctgtgct gatgactact acgcagagaa acaaatgtgc
    11401 aagagaaagc gctatatctc cccatgcaaa gagtgaaaaa tcgctttgaa aggccctcat
    11461 gatggggtta tatcagatca agcggtgaga aagcgtattt gcacaaatgt gcagaaccag
    11521 gaggcggcaa tggaaaacag tgacgatatc cgtttgattg tgaagattgc ccaactctat
    11581 tacgaacagg atatgacgca ggcgcaaatc gcgcgcgaac tgggtattta ccgcaccacc
    11641 atcagccgct tgcttaaacg aggccgcgat cagggaattg tcaccatcgc catcaactat
    11701 gactacaacg aaaatctctg gctggagcag caactgaagc aaaagtttgg cctgaaagac
    11761 gttgtggtgg tgtcgggaaa tgatgaggat gaagagactc aactggcgat gatggggtta
    11821 cacggcgcgc aactgctgga tcgcttgctg gagcctggcg atattgtcgg tttttcctgg
    11881 ggtcgcgcgg tgagcgcact ggttgaaaac ttgccgcagg cggggcaatc gcggcagtta
    11941 atctgcgtac caattattgg cggaccgtcc ggtaaactcg aaagccgcta tcatgtaaac
    12001 acattaacct acagcgcggc agcgaagctg aaaggggaat cgcatctcgc ggattttccg
    12061 gctctgctgg ataacccatt aattcgtaat gggatcatgc agtctcagca ctttaaaacc
    12121 atctctgcct actgggataa tctggatgtc gccctggtgg gaattggctc accggccatt
    12181 cgcgacggcg ctaactggca tgcgttttat ggtggtgaag agagtgacga cctgaatgcc
    12241 cgccaggttg ctggcgatat ttgctcgcgc ttttttgata ttcacggcgc aatggttgaa
    12301 acgaatatga gcgaaaaaac actctctatc gaaatgaata aattaaagca ggcacgatat
    12361 tccattggca ttgccatgag tgaagaaaaa tacagcggaa ttgttggtgc actgcgtgga
    12421 aaatatatta attgtctggt aacgaatagc agcacagctg aactattact gaaataaaaa
    12481 tcttcccgat gtttagcgta ataaaaatta cggcggggcc ctattatcta aaatcaggag
    12541 tctgttatgc aaacgtggtt aaatttgcag gataaaatca ttattgtcac cggcggcgca
    12601 tccggtattg gtctggcgat tgtagaggaa ttattagcac aaggcgcaaa cgtacagatg
    12661 gtagatattc acggtggcga tggtcaatat gaaggtcata aaggctatca gttttggccg
    12721 accgatattt ccagcgccaa agaggtaaat catacggtag cggaaattat ccagcgtttt
    12781 ggtcgcatcg acggtctggt caataacgcc ggggtcaatt tcccgcgtct gctggtcgat
    12841 gagaaagcgc ctgccgggca gtatgaactc aacgaagctg cattcgaaaa aatggtcaat
    12901 atcaaccaga aaggcgtttt tctgatgtcg caggcggtgg cgcgacagat ggtcaaacaa
    12961 catgatggcg tgattgtgaa tgtttcctca gaaagtggac tggaaggctc agaaggccaa
    13021 agctgttacg ccgctaccaa agccgcgctt aatagcttca cgcgatcctg gtcgaaagaa
    13081 ttgggtaaac acggtatccg tgtggtcggt atcgcgccgg ggattctgga aaaaacagga
    13141 ctgcgcacgc cggaatatga agaagcgctg gtgtggacgc gcaatatcac cgtcgagcag
    13201 ctgcgtgaag gctataccaa aaacgccatt cctattgggc gcgccggaag attagcagaa
    13261 gtcgctgatt ttgtttgtta tctgctgtct gaacgcgcca gctatatcac cggagtaacc
    13321 actaacattg cgggcggcaa aacgcgcggc taaggaggct ttatggtcaa tgccattttt
    13381 tgcgcccacg gcaaattagc ttgcgccatg ctggaatcgg ttcagatggt gtacggcgac
    13441 gccagggtcg aggcagttga atttgtgccc ggcgagaacg ccggagacat cgtagcaaag
    13501 ctggaaaagt tagtaagcat tcacaatcaa gatgagtggc tgattgccgt ggatttacag
    13561 tgcggcagcc cgtggaatgc ggcagcgatg cttgcgatgc gcaatcctcg tctgcgggta
    13621 atcagcggcc tttctctgcc gctggcgctc gaactggttg ataaccagga cagcatgaat
    13681 gttgatgaac tgtgtgaaca cctgacgcaa atcgcaaagc aaacctgcgt tgtctggaaa
    13741 caactggcaa cggcagagga ggatttctga tgaacattac gctcgcccgc attgatgacc
    13801 gcctgatcca cggtcaggtc actaccgtct ggtcgaaagt ggctaacgcc cagcgcatta
    13861 ttatctgcaa tgacgaagtt tataacgatg aagttcgccg gacattattg cgtcaggctg
    13921 cgccaccggg aatgaaggtt aacgtggtca atattgaaaa agccgtcgct gtttatcaca
    13981 acccgcaata tcaggacgaa accgtttttt atcttttcac tcgcccacag gatgccttag
    14041 cgatggttcg ccaaggggta aaaatagaca ccctaaatat tggcggcatg gcctggcggc
    14101 ccggtaaaaa acagttaacc aaagcggttt cattagatga tgatgatatt aacgcatttc
    14161 atgagttaaa taatcttggc gtcatactgg atttacgcgt cgtagcgtca gatccctcaa
    14221 tcaatattat cgacaaaata aacgaacagt taatcgcaaa ttaaataata gcgcttgtgt
    14281 acatatgctt taacaggcag ggatgacgac acttaaaagg tgaaaaatca tggaaataag
    14341 taccctacaa atcattgcta tatttctttt ttcctgtatt gccggaatgg gcagcgtgct
    14401 ggatgaattt cagactcatc gcccgttaat tgcctgtacg gtgattggtt taattctcgg
    14461 tgatttaaaa accggaataa tactcggtgg tacgctggaa ttgatagctc tcggctggat
    14521 gaacgtcggc gcggcgcaat ctccggattc tgcactcgcc agcataatct ccgccattct
    14581 ggttatcgtt ggtcagcaaa gcatcgccac cggaatcgcc atcgcgttgc ctgtggctgc
    14641 ggcaggccag gtgctgactg tgtttgcccg taccatcacc gtagtgttcc agcacgcggc
    14701 ggataaagca gcggaagaag cacggtttcg cactctcgat attctgcatg tctccgcgtt
    14761 tggcgtgcag gcgctgcgcg ttgctattcc ggcactgatt gtctcactgt tcgtcagcgc
    14821 cgatatggtg agcaatatgc tgagcgcgat accggaattc gtgacgcgcg gtctgcaaat
    14881 tgctggcggt tttatcgtgg tggtcggtta cgccatggtg ctacgtatga tgggcgtgaa
    14941 atatttgatg cctttctttt tcctcggttt cctcgcaggt ggctacctcg atctcagtct
    15001 gctggcgttc ggtggcgtcg gcgtgatcat ggccctggtc tacatccagc taaatccaca
    15061 gtggcgtaaa gctgaacctc aactccaggc caccgcctcc accgcccttg accaacttga
    15121 cgattaacgg agccatcatg gaacagagaa aaattacacg cagcgatctg gtgagcatgt
    15181 ttctgcgctc caacctgcaa caggcgtcct ttaactttga acgtattcac gggctgggtt
    15241 tttgctacga catgatcccc gccatcaaac gactttaccc attaaaagag gatcaggttg
    15301 cggcgctcag gcgacacctg gtgttcttca ataccacgcc agccgtatgt ggcccggtca
    15361 tcggcgtcac tgccgccatg gaagaggcgc gggccaacgg cgcggaaatt gatgacggta
    15421 ccattaacgg catcaaagtc ggtctgatgg gaccattggc aggagttggc gatccactgg
    15481 tctggggaac gctgcgcccg attaccaccg cgctcggcgc atctctggca ctttcgggca
    15541 acattctcgg cccgctgctg ttcttcttta ttttcaatgc ggtgcgtctg gcgatgaagt
    15601 ggtatggcct acagctcggc tttcgcaaag gggtgaatat cgtcagcgat atgggcggga
    15661 atgtgctgca aaaactcacc gaaggcgcgt cgattctcgg actgtttgtg atgggcgtgc
    15721 tggtcaccaa atggacgtca atcaacgtac cgttggtggt ttcacaaacg catgccgccg
    15781 atggctccac cgtcaccatg accgtgcaga acattctcga ccaactttgc cctggtttgc
    15841 tggcgctcgg tctgacgctg cttatggttc gtctgctcaa caaaaaaatt aacccggtat
    15901 ggctgatttt cgccctgttt ggcttaggga ttatcggtaa tgcgctgggc ttcctgtcca
    15961 gattcttcgc cccggcatga ctgccggggc catcgctcaa catgaggtgg tttatgaaaa
    16021 caacagctct gcgtctttat ggtaaacgtg atttacgcct ggaaaccttt gaccttcctg
    16081 aaatgcagga ggatgaaatc ctcgcgacgg tggtcactga cagcctgtgc ctctcttcct
    16141 ggaaagaggc caatctgggt gaaaaccata aaaaagtacc cgacgatgtg gcgaccaacc
    16201 ccatcatcat cggccacgag ttttgcggcg atattctggc cgtgggtaaa aagtggcagc
    16261 acaaattcca gccgggtcag cgttatgtga ttcaggccaa cctgcaactc cccgaccgcc
    16321 cggactgccc cggctactcc ttcccgtggg tgggcggcga agccacgcat gtagttattc
    16381 ccaacgaggt catggaacaa gattgcctgc tggcatacga cggcgaaacc tattttgaag
    16441 gctcactggt tgaaccgctt tcctgcgtga ttggcgcgtt caacgccaac tatcatcttc
    16501 aggaaggtag ttataaccac acgatgggga ttcgcccgca agggcgcatg ctgatcctcg
    16561 gcggcaccgg accaatggga ctgttggcga ttgattatgc gctacatgga cccgttaacc
    16621 cgtcgcagct cgtcattacc gataccgaca acgataaatt gagttatgcg cgcaagcact
    16681 atccatcaga accgcaaaca ctgattcatt atctcaatgc cgccgatgca gcatttgata
    16741 cgctaatggc gctgagtggc ggtcacggct tcgatgatat tttcgtcttt gtgcctaatg
    16801 aaggactggt gactctcgcc tcttccttgc tggcgacaga tggttgcctg aatttcttcg
    16861 ccggaccgca ggataaacat ttcagcgcgc caattaattt ctacgatgtg cattatgcat
    16921 ttacccacta cgtgggcacg tcaggcggca ataccgacga catgcgcgcg gcggtcaaac
    16981 tgattgaaga gaaaaaagtg caggccgcaa aagtggtaac acatattctt gggctgaatg
    17041 ccgcgggcga aaccacgctt gaattgcctg ccgtcggcgg cggcaaaaag ctggtgtata
    17101 ccgggaaata cctgccgctg acgtcactca cgcagattca ggatcaagca ctggcggcga
    17161 ttctggcgcg tcatcagggg atctggtccg gtgaggcgga gcaatatctg ctcactcatg
    17221 cagaggcaat ttcccatgat taatcgcgct acttaactct gcatgtcgct ggctggtcgc
    17281 cgcggcaact tcgcgacctg atgtgcggct gattgctgaa gcggcccaat gtgcacgaga
    17341 aggataaaaa aacatcagcg ccttaagggg cgctcaaatt ggtggcaaag tacacattgt
    17401 ttatgccgga tgcggaataa agccttattc ggcctacata atctcgaaaa ttcaatatat
    17461 tgcagttatc tggtaggcct ggtaagcgta gcgcatcagg caatgctgtg tttgtcatca
    17521 gtctcagctc ccggtgcaat acgtgacatc gtgagccatg tcagtaagtg gttaaattcg
    17581 cagtggtagc atgccttccg tgatttctta ctaaccggag aagtaatgga actgctttta
    17641 ttgagtaact cgacgctgcc gggtaaagcc tggctggaac atgcactgcc gctaattgct
    17701 gaacagttgc agggtcgccg ctcagcggtg tttatccctt tcgctggcgt aacgcagacc
    17761 tgggatgatt acacagcgaa aacggctgcg gttctcgctc cgctgggtgt ttctgtcacc
    17821 ggtattcata gcgttgtcga tcccgttgcc gcgattgaaa atgctgagat cgtgattgtc
    17881 ggcggcggga atactttcca gttgctgaaa cagtgccgcg agcgcgggct gctggcacca
    17941 attactgacg tggttaaacg tggtgcgctg tatattggct ggagcgcagg cgctaacctt
    18001 gcttgcccaa ccattcgtac caccaacgat atgccgattg tcgatccgca aggtttcgat
    18061 gcgctaaatc tgttcccgtt gcaaatcaac ccgcacttca ccaacgcgct gccggaaggc
    18121 cataaaggtg aaacccgtga gcagcgtatt cgcgaactgc tggtcgtcgc gccagaactg
    18181 acgattattg gtctaccgga aggtaactgg atcacagtga gtaaaggtca cgctacgctg
    18241 ggtggcccga acaccaccta tgtgtttaag gccggtgaag aagcggttcc gctggaagct
    18301 ggtcaccgtt tttaagtgaa ttgccggatg catgatccgg catcttgttc atagtgcctg
    18361 atgcgacgct tgacgcgtct tatcaggcct acaggattca caatattaat actcgtcccg
    18421 tccttcatct tcatccgact gttccagcac actgtaagcc accgaacaaa acagcgagtt
    18481 caggcgctgc atatctccca gtaagcctaa atgtagcgaa ctggtttcaa tgctctgcac
    18541 gttttgctga tgcaggcgat cgacgtgggc gtgggaatag cggcgattaa gaatgcgaaa
    18601 gcgatgctta ctacggcgca aacgacgagc gctggtgaca tcgccagaga agaacaccga
    18661 cattgccagc tttaaattac tgagcaattg ctcatagagc gcatccagtt ccttcaaccc
    18721 atcaagcgaa aatgcccgcc gtgctgccag tgatttgtca gcaatttcgc tgcccatgcg
    18781 ctcgacgata tcggaggcct gttcaaggtt gagcgacatt tcaataatct ccgcccagcg
    18841 gcgcgactct tcctccgcca gctcttcttt tggcatccgc gccagataca gcttaatggc
    18901 ggtgtagaga acgttgatat catctgccag cttacgcagc tctttctcct gccgtggctc
    18961 accgtgcatc actttattca acccttccat catctgttcc atggcgtcgc caatgcgcag
    19021 ggtttcgcgc gcggcgttcg ccagagcaag cgtgggcgta tccagcgcgc tgacatccag
    19081 atgtttaggc cgtagctggg tatccagttc cggttcatcg cgaataatcg ttttgcaaaa
    19141 ccgtgccatc gggtcaacaa atggcagcat gaccaggcaa cgtacaaggt tgtagaagac
    19201 gtggaaatag atcaccagtt ccgcttttgg caatgacagc ttccccattg tctctgccag
    19261 caaatggaca aacggcagga taatcaggct acccaccagc ttaaacagca gactacccag
    19321 cgcgacacgg cgggctgcgg cattggcggc actgttgttg agcatcgcca gcaggccgga
    19381 accgaggtta gcaccaatca ccagacagag cgccacgggg aaggagataa tgcctgcggc
    19441 ggtcagagcc gcggtcagca gtacagcagc aaggctggag tagctgataa tggcgaacat
    19501 cgcgccaatc agcgcatcca gcagaatatc gccggtcagc gaggcaaaga tcacctgaac
    19561 gccgtttgcc tgggtgatcg gcgttacggc ctgcacaatc aactccagcg ccagcaaaat
    19621 cagcccaaga ccaataccga cgcggcccag ttgcccggcg cgtgactgtt tgcgtccgag
    19681 gaaaaaaatc acgccgataa aaataagtaa cggtgacagc caggataagt cgaaggtgag
    19741 aatacgcgcc attagcgccg tcccgacatc tgcccccagc acaatgacca gagccggtgc
    19801 gagggctacc agatcctgag cgacgaacga ggtcaccagc atggtggtgg cattactgct
    19861 ctgtaccagt gcggtaacgc cgatccccgc gcaaaaggcg agcggctttt tttcgacgct
    19921 ccggctaagg acagtacgca aacgcgcgcc gaagacgcgc attacgccgg ttcgaacaat
    19981 atgagtcccc cagaccagca gggcgacggc agaaagcagg tgaagcagcg ttaacacgag
    20041 ttcaggttct ccttatcgtt atatgttcct gaagcaattg aatgaccaaa ggaccggaaa
    20101 cacgctgact ggcatcttta ctgtgccttt tgacattcgt ttttccggct aagattattc
    20161 cagcctgatt ccagtataag tgtttcatat taataaagag acagggcact cattgagcgc
    20221 cctgtttgtt gtaaggaaaa gtgacagatt tgtgaatcag tccgcgtcat accccagatt
    20281 cggtgccagc cagcgctcaa catctgagac gctcatacct ttacggcggg catagtcttc
    20341 aacctgatcg cgctgaattt gcgccacggc gtagtacttg ctgtccgggt ggctgaaata
    20401 ccaacccgaa accgatgcac cgggccacat ggcgaaagat tctgtgagtt tcatgccggt
    20461 atgtttttcc acttccagca gctcccagat ggtggctttt tccgtatgtt ccgggcaggc
    20521 cgggtagccc ggtgccggac ggatgccctg gtagttttcg cggatcagtt cttcgttgct
    20581 gaggttctcg ttcggcgcat agccccagta gactttacgc acacgctcat ggagatactc
    20641 cgcaaaggct tcggctaaac ggtcggcaag cgctttcacc atgattttgt tgtaatcatc
    20701 gtgctgcgct tcaaaggcat cagccagtgc gtcctcttcc agcccgccag tcacggcaaa
    20761 tgcgccgatg taatctgctt taccagaaag cttcggcgca acaaagtcag cgagacagta
    20821 gttagcgaat cccgtttttt cggtctgttg acgcagatgg tggctgacat tgatcacatg
    20881 ggtgcgcgtt tcgtcacggt agatttcaat gtcatcgccc acacggtttg ccgggaacag
    20941 gcccaccacg ccacgcggat tcagcgtttt ctcggcgctt aatttatcca gcatgtcgtt
    21001 ggcgtcttta aacagccgct gcgcctcaac gcccaccact tcatcttcca gaatgcgcgg
    21061 atacttcccg gccagcgacc aggtcataaa gaacggtgtc cagtcgatgc aattacgcag
    21121 agtttcgatg ctggcttcaa cttcctgcac gccgagacgg tgcgccaccg gcggcgtgta
    21181 agcctgccag tcaaaagcga agtcgttatc gcgcgccgct tccagcgtga ccggtggtgt
    21241 gcgcggtttc ttgcgcccgt gctgaatacg tacggtttcg tactccttgc gggtacgagc
    21301 gacaaaatca tcgcgctgag tatcggaaag cagcgccgcc accacaccaa cggtgcgcga
    21361 ggcattctgc acatacaccg tcgggccgct gtagttctgc tcaattttca ccgccgtgtg
    21421 cgcttttgag gtggtcgcgc caccgatcag taacggaatg gtgaagccct gacgctccat
    21481 ctctttcgcc acgttaacca tctcgtccag cgacggcgtg ataagccccg aaaggccaat
    21541 caaatcagca ttcacttctt tagcagtacg gagaattttt tccgcaggca ccatcacgcc
    21601 gagatcgaca atttcgtagt tgttacattg cagcaccacg ccaacgatat ttttaccgat
    21661 atcgtggacg tcgcccttca cggtggcgat caccatcttg ccgttggttt tgccctgctc
    21721 tttgctggct tcaataaacg gttcgagata cgccaccgcc tgtttcatga cgcgcgccga
    21781 tttgaccacc tgtggcagga acattttgcc ttcgccaaac agatcgccga ccacgttcat
    21841 gccgtccatc aacgggcctt caatcacttc aatcgggcgc gtagcctgct ggcgggcttc
    21901 ttcggtatcc tgctcgataa actcggtaat gcctttgacc agcgagtatt ccagacgttt
    21961 attcacttcc cacgagcgcc actccgcctg ctgggcgtta tcggtgtcgt cggttttgct
    22021 gccgcgatat ttctcggcaa gctccagtaa acgctcagtg ccatcgtcac gacgattaag
    22081 aatcacatct tccaccgcgt cgcgcagttc agcgggtagg tcgtcgtaaa tcgccagttg
    22141 ccccgcgttg acgatcccca tatccatgcc attgcgaata gcgtagtaga ggaacactgc
    22201 gtgaatggct tcgcgcaccg gatcgttgcc acggaatgag aaagaaacgt tagatacgcc
    22261 gccggaaatc agcgcgtgcg gcagttcgcg tttgatgtct tcacacgcgc cgataaagtc
    22321 ctgcgcgtag ttgttgtgct cttcaatacc ggtggcgacc gcgaagatgt tcgggtcgaa
    22381 gatgatatct tctggcggga agccaacctc ttcggtgagg attttgtacg cccgacggca
    22441 aatctcgatt ttccgtgcgc gagtatcggc ctgtccctgt tcatcaaagg ccatcaccac
    22501 cactgccgca ccgtagcgac gcaacaattt cgcgtgatgg ataaaggcgt cgacgccctc
    22561 tttcatcgag attgagttaa caatgccttt gccctgaata cacttcagcc ctttttcgat
    22621 aacgtcccat tttgaggagt cgatcataat cggcacgcga gcgatatccg gttcaccggc
    22681 aatcagattg agaaaacgca ccatcgccgc ttcggcatcg agcatccctt catccatgtt
    22741 gatatcgata atctgcgcgc cgttttccac ctgctgacgc gccacatcct gcgcctcgct
    22801 gtatttctct tctttgatca ggcgcttgaa cttagcagag ccggtgacgt tggtgcgttc
    22861 acccacgttc acaaacaggc tatcgtcgcc aatgttcagc ggctccaggc cagacaaacg
    22921 gcaggctacg ggaatttccg gcagtttgcg cggcgctaat ccttctactg cacgactcat
    22981 cgctgcaata tgttgcggcg tggtcccgca gcaaccaccg acgatattga gaaagcccgc
    23041 ttgcgcccat tcacgtatct gttttgccat cgtgtcggcg tcgagatcgt attcaccaaa
    23101 ggcgttaggc agcccggcgt tcgggtgggc ggtgacgtag cattccgcaa tccgtgatag
    23161 ttcctgcacg tactggcgca gttcatcggg ccccagcgca cagttcaggc caaaggttag
    23221 cgcttcggcg tggcgcaatg agttgtaaaa tgcttcggtg gtctgcccgg agagcgtgcg
    23281 cccggaggcg tcggtgatgg tgccggagat cataatcggc agctcaacgc ccagcgcttc
    23341 aaactccgtt ttcaccgcaa aaaccgccgc tttggcgtta agggtgtcga aaacggtttc
    23401 aatcaggatc agatccgcac caccttccac cagcgctttg gtggattcgc aataggccgc
    23461 caccagctgg tcaaaagtga tgttgcggaa tgccggatcg ttgacgtccg gagaaataga
    23521 cgccgtgcgg ttggttgggc cgagaacacc ggcaacgtag cgcggtttct ccggcgtgcg
    23581 cgcggtccac tcgtcagcac aggcgcgcgc cagtttcgcc gccgcaaagt tgatttccgc
    23641 cgacagggat tccatctggt aatccgccat cgcaatggtc gtggagttga aggtgttggt
    23701 ttcgatgata tccgcgcccg cttcaaagta ggcgttgtgg atagcggcga tcacttccgg
    23761 tttgctgagc accagcaggt cgttgttgcc tttgagatcg catggccagt cggcaaagcg
    23821 ttcaccacga aaatcggctt cgttcagtcg ataactctgg atcatggtgc ccataccgcc
    23881 gtccagcacc agaatacgtt catttaactg cgcacgcagt tgttccactt tgctgctcac
    23941 acttgctccc gacacgctca acccagattt aataaaaatt caacaaacca tactggcata
    24001 aacgcatctg tggtaaaagc gaccaccacg caacatgaga tttgttcaac attaactcat
    24061 cggatcagtt cagcaactat tgcattagct aacaataaaa atgaaaatga ttcccacgat
    24121 gcagaaaaag gagactgtca tggtcgcacc cattcccgcg aaacgcggca gaaaacccgc
    24181 cgttgccacc gcaccagcga ctggacaggt tcagtcttta acgcgtggcc tgaaattact
    24241 ggagtggatt gccgaatcca atggcagtgt ggcactcacg gaactggcgc aacaagccgg
    24301 gttacccaat tccacgaccc accgcctgct aaccacaatg caacagcagg gtttcgtgcg
    24361 tcaggttggc gaactgggac actgggcaat cggcgcacat gcctttatgg tcggcagcag
    24421 ctttctccag agtcgtaatt tgttagcaat tgttcaccct atcctgcgca atctaatgga
    24481 agagtctggc gaaacggtca atatggaggt gcttgatcaa agcgatcacg aagcgattat
    24541 tatcgaccag gtacagtgta cgcatctgat gcgaatgtcc gcgcctatcg gcggtaaatt
    24601 gccgatgcac gcttccggtg caggtaaagc ctttttagcc caactgagcg aagaacaggt
    24661 gacgaagctg ctgcaccgca aagggttaca tgcctatacc cacgcaacgc tggtgtctcc
    24721 tgtgcattta aaagaagatc tcgcccaaac gcgcaaacgg ggttattcat ttgacgatga
    24781 ggaacatgcg ctggggctac gttgccttgc agcgtgtatt ttcgatgagc accgtgaacc
    24841 gtttgccgca atttctattt ccggaccgat ttcacgtatt accgatgacc gcgtgaccga
    24901 gtttggcgca atggtgatta aagcggcgaa ggaagtgaca ctggcgtacg gtggaatgcg
    24961 ctgacttttt ctggcgggca gaggcaatat tctgcccatc at
//
LOCUS       DADLFA010000044        24917 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000044 DADLFA010000000
VERSION     DADLFA010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24917
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.044"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(38..376)
                     /locus_tag="JQH65_001519"
                     /pseudo
     CDS             complement(38..376)
                     /locus_tag="JQH65_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(492..1043)
                     /gene="maa"
                     /locus_tag="JQH65_001520"
     CDS             complement(492..1043)
                     /gene="maa"
                     /locus_tag="JQH65_001520"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HAY6138982.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKGVPDNVVVGGNPARIIKKL"
     gene            complement(1215..1433)
                     /gene="hha"
                     /locus_tag="JQH65_001521"
     CDS             complement(1215..1433)
                     /gene="hha"
                     /locus_tag="JQH65_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HAY6138983.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(1459..1833)
                     /gene="tomB"
                     /locus_tag="JQH65_001522"
     CDS             complement(1459..1833)
                     /gene="tomB"
                     /locus_tag="JQH65_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HAY6138984.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNYGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(2379..5528)
                     /locus_tag="JQH65_001523"
     CDS             complement(2379..5528)
                     /locus_tag="JQH65_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HAY6138985.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(5551..6744)
                     /gene="acrA"
                     /locus_tag="JQH65_001524"
     CDS             complement(5551..6744)
                     /gene="acrA"
                     /locus_tag="JQH65_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HAY6138986.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDNKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTAPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            6886..7533
                     /gene="acrR"
                     /locus_tag="JQH65_001525"
     CDS             6886..7533
                     /gene="acrR"
                     /locus_tag="JQH65_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HAY6138987.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            7661..11023
                     /gene="mscK"
                     /locus_tag="JQH65_001526"
     CDS             7661..11023
                     /gene="mscK"
                     /locus_tag="JQH65_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HAY6138988.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLELQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIVGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYIDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(11235..11396)
                     /gene="rsmS"
                     /locus_tag="JQH65_001527"
     CDS             complement(11235..11396)
                     /gene="rsmS"
                     /locus_tag="JQH65_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HAY6138989.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(11410..11937)
                     /gene="priC"
                     /locus_tag="JQH65_001528"
     CDS             complement(11410..11937)
                     /gene="priC"
                     /locus_tag="JQH65_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HAY6138990.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRTT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTH"
     gene            12007..12384
                     /locus_tag="JQH65_001529"
     CDS             12007..12384
                     /locus_tag="JQH65_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HAY6138991.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            12537..13088
                     /gene="apt"
                     /locus_tag="JQH65_001530"
     CDS             12537..13088
                     /gene="apt"
                     /locus_tag="JQH65_001530"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAY6138992.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            13217..15088
                     /gene="dnaX"
                     /locus_tag="JQH65_001531"
     CDS             13217..15088
                     /gene="dnaX"
                     /locus_tag="JQH65_001531"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAY6138993.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVPSALEKAP
                     AKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELAAKLAAEAIERDPWAARVS
                     QLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGAQQKLAEALSTLKGSTVEL
                     TIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQTLRRFFDAELDEESIRPI"
     gene            15141..15470
                     /locus_tag="JQH65_001532"
     CDS             15141..15470
                     /locus_tag="JQH65_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAY6138994.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            15470..16075
                     /gene="recR"
                     /locus_tag="JQH65_001533"
     CDS             15470..16075
                     /gene="recR"
                     /locus_tag="JQH65_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAY6138995.1"
                     /translation="MQTSPLLTQLMEALRCLSGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            16185..18059
                     /gene="htpG"
                     /locus_tag="JQH65_001534"
     CDS             16185..18059
                     /gene="htpG"
                     /locus_tag="JQH65_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HAY6138996.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            18240..18884
                     /gene="adk"
                     /locus_tag="JQH65_001535"
     CDS             18240..18884
                     /gene="adk"
                     /locus_tag="JQH65_001535"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAY6138997.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            19016..19978
                     /gene="hemH"
                     /locus_tag="JQH65_001536"
     CDS             19016..19978
                     /gene="hemH"
                     /locus_tag="JQH65_001536"
                     /EC_number="4.99.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HAY6138998.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEYIEMMANLVAAYR"
     gene            complement(19975..20934)
                     /gene="aes"
                     /locus_tag="JQH65_001537"
     CDS             complement(19975..20934)
                     /gene="aes"
                     /locus_tag="JQH65_001537"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HAY6138999.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIVGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            21086..22390
                     /locus_tag="JQH65_001538"
     CDS             21086..22390
                     /locus_tag="JQH65_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HAY6139000.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(22523..24199)
                     /locus_tag="JQH65_001539"
     CDS             complement(22523..24199)
                     /locus_tag="JQH65_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HAY6139001.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(24437..>24917)
                     /locus_tag="JQH65_001540"
     CDS             complement(24437..>24917)
                     /locus_tag="JQH65_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY6139002.1"
                     /translation="SYYTFYLMQKFGLSIQNAQLHLFAFLFAVAAGTVIGGPVGDKIG
                     RKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFSAILVYAQELLPGRIG
                     MVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLLGMLTIFLPDNRHKD"
BASE COUNT         6229 a         6493 c         6396 g         5799 t
ORIGIN      
        1 aacaaatggt agtgtccgct attgccagta cacctcacag cgcggcaggt acaaagcgcg
       61 tgttgtacca ttcgccgtta tagcaaacgc ggaaatccag cacgtcgata tcttcatagc
      121 ggtagcgtgg gtagacatcg aaaaagaaga aaccattaag caggataaat aatacaacca
      181 gtagccagac agagcctgac agcgtttcgg actgcaacat caccgccagg gtggcaaaaa
      241 aagcaacgta cataccgata aacagccccg gatgtttacg gataaaactg atactaaagc
      301 ggggtttgtt gtcgcgtttt tcgcgggtat tcagagactc aatcgtttcg gtcagcaggc
      361 gttgtatttc ggtcatcgtt tgccttcgtg gtatctgcaa ctttccaggg aacaccctat
      421 tttaacgtgg gaatagtaga ggggaagtaa cagatctacc acaattttgc ataacagttg
      481 cgaaaaaccg attacaattt tttaattatt ctggctggat taccgcccac gacaacgttg
      541 tccgggacac cttttgtgac aactgcacct gaagctacca cgacgttatc accaatggtc
      601 acaccagggt taatgaccgc gcgtccgcca atccagacgt tattaccgat ggtgacgggt
      661 ttccccagtt cagcaccgct attacgtgct acagggtcga tgggatgtgt tgccgtgtag
      721 atatgaacgc ctggtgccaa catacagtta tcaccgatgc gaatagggca gacatcaagc
      781 atcacgcaat cgaagttggc gaaaaaatta ttgccgagaa aaatgttata gccataatca
      841 cagcgaaacg ttggctcaat ataagcctct gtcacctgac cgaataaatc agcgagaatt
      901 tgctggcgta atgtgtgctc ttccgccagg gaatgattgt atcggtgaat aagctgacga
      961 gcgcgcaggc gatcgcgaga taacgtctca tctgccgagc gatacaactc accagcaatc
     1021 atcttttctt tttctgtgct cattactgac ctccggttgc tgaaaatgcg gcaacacagt
     1081 aagacgaaaa gtcagtgaac agaacgttcc cattacatct gtgtgataaa gatcacatag
     1141 ggtatgtctt catggtgaaa aagtataaaa ttcttaataa acagccggtt atagctccga
     1201 aagcgaatta tttattagcg aataaatttc catactgagg aagggatctt gtcgtatagt
     1261 ttattcatgg tcaattcggc gaggcggtga tctgcggctg agtaaaatac cgccagttca
     1321 ttatctgata attcgtattt atttttctcg ataacacgct ccagcgtgtc aattgtctgg
     1381 caacgacgta aacgcattaa ataatcggtt ttcgttaaag gtttttcgga catacttcta
     1441 cccatggttg taataattct aacaagataa actcgcagga ttctctttcg tcgcattgac
     1501 aaaacaacgg aatagtcgat tacctgactt ccgccatttc tgaagatcct gcatattaat
     1561 accataacta ctgaacaaca taaaggtgtc atccagatat tcgtcgatct gctcaatgag
     1621 cttattgtct tcattatact taattttgta attaagtgcg aaggtcgcaa tatgctcaat
     1681 cagttcattc aactggaggt tgatcgccga ggttgggtcg ttaacccagc cataattgct
     1741 ttcttcaagg tttgcaaggc agtcatgata cagggtttca cagagaaact taagctgtgc
     1801 gatatcatgt cttttgggtg agtattcatc cataacgcgt ccccttctta gcggttgaac
     1861 taacggacac ctttcgggat ggaaaaaact tactgacctg gacttgccct ctcgtttgtt
     1921 agcttaacta taagccactc tttgcaggta ttcattgcat tattacgaaa aattacaatt
     1981 aggaaggaat cgggacagga ctcacttttt catactagca ctaacccgca gcaggtaaaa
     2041 gcagtttgct cgaactgttc ataacatttc aggattatcg ccagatgaaa ttggtgacac
     2101 taataccagg attgctctga atatgacgta ataaccgagg aatgaataaa gaattaccgc
     2161 aaataattaa gaatagcctc taaatgatta tggattatta aatctattag cgctacttaa
     2221 tataaattaa ctaacaatca gtatttttta tgaattttat ccgtggttaa tactggtttt
     2281 cgtatgagat cctgagttgg tggttcaatt actccttaat gttcgtaggt tatgcataaa
     2341 aaaggccgct tgcgcggcct tagtgattac acgttgtatc aatgatgatc gacagtatgg
     2401 ctgtgctcga tatcttcatt cttgcggcta aagcggcggc gaaccaccac aaagaatacc
     2461 ggaacgaaga agattgccag taccgttgcg gtcaccatcc cgcccattac accggtacct
     2521 actgcgttct gcgcgccgga accagcacca gtactgataa ccagcggcat aacgccgagg
     2581 ataaacgcca gcgaggtcat caggatcgga cgtaaacgca tccgcactgc atcaagcgtc
     2641 gcttcaatca gacctttacc ttctttatcc atcaagtctt tggcgaattc gacgataagt
     2701 atcgcgttct tcgccgacaa cccaatggtt gtgagcaggc ctacttggaa gtaaacgtca
     2761 ttggtcaggc cacggaaggt ggcagccagc aacgcaccga taacccccag cggaacgacc
     2821 agcataacgg agaacggaat cgaccagctc tcgtacagcg ccgctagaca caggaatacg
     2881 acaatcaacg aaatcgcgta cagtgaaggt gcctggttgc cggagaggcg ttcctgatag
     2941 gacatccccg tccagtcata gccaacaccg gtaggcagtt tgctcgccag ttgttccatt
     3001 agctccattg cttcaccggt acttttaccc ggtgccgcct ggcctaagat ttccatggat
     3061 ggcaggccat tgtaacgttc cagacgcggc gaaccgtact cccaacgaga agaggagaat
     3121 gccgagaacg gcaccatctg accatcagca gcacgaacat accagtcgcc gatatcatcc
     3181 ggcagcatac ggtatttcgc ttctgacatg acgtaaactt tcttcacacg accgcggtcg
     3241 ataaagtcgt tcacatagct accgccccat gcagcgccca gagtggtgtt aatgtcgttg
     3301 atagaaacac ccagcgcctg cgctttttcc tggtcgatat caatcttaaa ctgcggggta
     3361 tcttccagac cgtttggacg tacgctggtc aacatatcag gatgctttgc tgcttctgca
     3421 agcagctggt tacgcgcctg agtcagtttt tcgtgaccaa ggccagcctg gtcaatcagc
     3481 tcaaagtcaa agccggttgc agtacccagt tccacgattg cgggcaggtt aaaggcgaaa
     3541 accatcgcat ctttgatttg cgagaaagcg cgtgttgcac gcatggtaat cgcttcaact
     3601 ttgttttctt cgcccggacg atcggcccag tccttcaagg aaacgaacgc aataccggta
     3661 ttctgaccac gtcccgcaaa gccgaagccg ttaacggcga acaccgactc aacgttgttc
     3721 ttttctttgg tcagatagta atgcgttacc tcattgagca ctttctgcgt acgttcctgc
     3781 gttgcacctg ctggcagctg aaccatggtc ataaatacgc cctggtcctc atctggcaag
     3841 aaggagcttg gcagacgcac gaacagatag gccatgccga ccacgatgat cagatacagc
     3901 accaggtaac gccccgtact gcgcagaata ccgcctacgc tgtcggtgta gtggtgcgtg
     3961 ctcttctcga acatgcggtt aaaccagccg aagaagcctt ttttaccttc cccgtgatcg
     4021 cctttggcaa tcggtttcag catggtggca caaagagccg gagtcaggat caacgctacc
     4081 agtaccgaca gcgccattgc tgaaacaatg gtaatagaga actgacgata gatagcacca
     4141 gtagaaccgc caaagaaggc catcggtacg aataccgctg acagtaccat cgcgataccg
     4201 accagagcgc cctgaatctg ccccatcgac ttacgggtag cttcttttgg cggcaaacct
     4261 tcttccgcca taacacgctc cacgttttct accacaacga tagcgtcatc caccaacaag
     4321 ccgatggcga gcaccatccc gaacattgtt agcgtgttta tcgagaagcc aaaggcggca
     4381 aggacggcaa aggtcccgag caataccacc ggtacggcaa tggtcggaat caacgtcgcg
     4441 cggaagttct gcaggaacag atacataacc aggaacacga ggatgatcgc ttcgaccagc
     4501 gttttaacca cttcgtgaat agagattttc acgaacggcg tggtgtcgta cgggtaaaca
     4561 attttcagac ccgacgggaa gaacggttcc atcttcgcca gttcagcacg gattgccgca
     4621 gcggtatcca gcgcatttgc accggtcgcc agcttgatcc ccagaccgga agccggttgg
     4681 ccgttaaact ctgcgatgat gtcgtagttc tcaccaccca gctcaatctt cgccacatca
     4741 cgcagcagga cgcgggaacc atcctgattc actttcagca ggattttgcc gaactcttca
     4801 gtagaggtca gacgcgtctg agcaataata gaggcgttaa gctgttggcc tttcaccggc
     4861 ggcgtaccac cgagctgacc cgccgcaacc tgggcgttct gcgctttgat ggcggtaatg
     4921 acatcaaccg gcgttagctg gaatttgttc agctcattcg ggttcatcca gatacgcatc
     4981 gcgtactgtg aaccgaacaa ctgaacatca cccacgcccg acgtacggct gatggcatct
     5041 ttcatattcg ccgctacgta gtcagagata tcctcctgcg tcatggtgcc atcggtgttg
     5101 ataacgccga caaccatcag gaagctgctg gatgatttct caacgctcac cccttgctgc
     5161 tgaacttctt gcggcagcaa cggcatcgcc agctgcagtt tgttctgcac ctgaacctgc
     5221 gcgatatccg catcagtacc agactcaaag gtcagggtga tctgcacggt ccccgtggag
     5281 tcactgttag aggacatgta catcaggtta tcgataccgt tcatattctg ttcgataacc
     5341 tgtgtcaccg tgtcctgcac tgttttcgca tcagcgccgg ggtaggaggc ggagatcgtt
     5401 actgccggcg gtgcaatcgt aggatattgc gccaccggca gtttgaggat cgccagcccc
     5461 cctgccaaca tgatgataat ggcgatcacc cacgcaaaaa tcgggcgatc gataaagaaa
     5521 ttaggcatgt cttaacggct cctgtttaag ttaagacttg gactgttcag gctgagcacc
     5581 gcttgcggct tgctggttat tatcagcggt aacttcttgt gcttttacct ggacaccagg
     5641 acgcactttc tgcagcccac ttattactac gcgatcgcct gctttcagac cttctgtcac
     5701 cagccactta tcgccaatag cctggcttgc aacgatcgga cgggtttcca ctttgtcatc
     5761 cgcgccaact accagtacgg tggcatcacc acgcggcgta cgggttacgc cctgttgcgg
     5821 gactaaaata gcgtttggat taagcccttc ttccagacgt gcgcgcacga acatacccgg
     5881 cagcagagtg tgatccgggt tcgggaagat agcgcgtagg gtgatagacc cagtggtctg
     5941 atcaacggta acgtcagaga attccagcgt accgtcctgc gggaacttaa tgccgtcact
     6001 ggtgatcagc gacactttgg ctttgccgtt ctcttgtttc agcgtgccat tcgccagttc
     6061 ctgtttcagg cgcaggaagt cgttgctgga ctgggtcaca tcaacgtaga tcggatcaag
     6121 ttgctgcacg gttgccagcg cagtcgcctg accgttctgt accaatgcgc cttccgtcac
     6181 gttcgactta ccaatgcgac cgctaatcgg agcggtgact ttggtgtaag ccagattgat
     6241 ccgcgcagtt tcaacggcag ctttcgccgc agttaccgca gcattcgcct gttgcgcatc
     6301 agccagagcc tgatcgtact cttgcttact gatgtactga gtaccgagca gtttctgata
     6361 acgattcacc gtcaattgcg cgatattggc tgcagcctgg gctttcgcca gatcaccttt
     6421 cgcactgtcg tatgtcgcct gataggtcgc aggatcaatc tgatagagag agacaccagc
     6481 ttcgatgtcg ctaccttctt tgaaattacg cttcaggata atcccgctaa cttgaggacg
     6541 aacttctgcg atccggtagg cactggtgcg acccggaagc tcggttgtga tctgcagagg
     6601 ttcagttttg actgttacta cgccaacggc gggcatctgc tggccacctt gttgggcctg
     6661 tttgttgtca catcctgtta gggctaagct gcctgagagc atcagaacga ccgccagagg
     6721 cgtaaaccct ctgtttttgt tcatatgtaa acctcgagtg tccgatttca aattggtcaa
     6781 tggtcaaaag ttaataaacc cattgctgcg tttatattat cgtcgtgcta tggtacatac
     6841 attcacaaat gtatgtaaat ctaacgcctg taaattcacg aacatatggc acgaaaaacc
     6901 aaacaagaag cgcaagaaac gcgccaacac atcctcgatg tggctctacg tcttttctca
     6961 cagcaggggg tatcatccac ctcgctgggt gagattgcaa aagcagctgg cgttacgcgc
     7021 ggtgcaatct actggcattt taaagacaag tcggatttgt tcagtgagat ctgggaactg
     7081 tcagaatcca atattggtga actagagctt gagtatcagg caaaattccc tggcgatcca
     7141 ctctcagtat taagagagat attaattcat gttcttgaat ccacggtgac agaagaacga
     7201 cgtcgattat tgatggagat tatattccac aaatgcgaat ttgtcggaga aatggctgtt
     7261 gtgcaacagg cacaacgtaa tctctgtctg gaaagttatg accgtataga acaaacgtta
     7321 aaacattgta ttgaagcgaa aatgttgcct gcggatttaa tgacgcgtcg ggcagcaatt
     7381 attatgcgcg gctatatttc cggcctgatg gaaaactggc tctttgcccc gcaatctttt
     7441 gatcttaaaa aagaagcccg cgattacgtc gccatcttac tggagatgta tctcctgtgc
     7501 cccacgcttc gtaatcctgc cactaacgaa taaccctgaa tctgactcca ggatttttcc
     7561 tggacatttt cgtcgttgct attctggttc actgcgtcgt gatattcttg cggtttgact
     7621 ttttcaggtc gttcttcagg ttcagaaacc ttcattcatc atgactatgt tccagtatta
     7681 caaacgatca cggcattttg ttttttcagc atttattgct tttgtttttg tcttgttatg
     7741 ccagaacacg gcgtttgcgc gggcgtcatc gaatggtgat ctgccgacaa aagcggacct
     7801 gcaggcgcaa cttgactcac taaataaaca aaaagatctt tctgctcagg acaaactgga
     7861 gctgcaggat ctgacagata cattagccac cctcgataaa atcgatcgca taaaagaaga
     7921 gacagttcag ctacggcaaa aagtcgctga agcgccggaa aaaatgcgcc aggcgaccgc
     7981 ggcgttaaca gcacttagcg atgtcgataa cgacgaagaa acgcgcaaaa ttctgagtac
     8041 gctttcgttg cgccagctgg aaactcgcgt tgcccaggcg ctggacgatt tgcaaaacgc
     8101 acaaaacgat ctggcgtctt ataacagcca gctggtttcg ttacagacgc agcccgaacg
     8161 cgtgcaaaat gcgatgtata acgcttcgca gcagctgcaa caaattcgca gtcgtctgga
     8221 tgggactgat gtcggcgaga ctgccttacg tcccagccag aaagtgttaa tgcaggctca
     8281 gcaggcgttg ctgaatgcgg agattgacca gcagcgtaaa agcctggaag ggaacaccgt
     8341 cttgcaggat accttgcaaa agcaacgtga ttacgtgacg gcgaacagcg cccgtctgga
     8401 gcaccagttg cagctgttgc aagaagcggt aaacagcaag cgcctgactt taactgaaaa
     8461 aacggcgcag gaagccgtct ccccggatga agccgcgcgt attcaggcta atccgctggt
     8521 gaaacaggaa ctggaaatta accagcagtt aagtcagcgt ctgattaccg cgactgaaaa
     8581 cggtaatcag ttgatgcagc aaaacattaa agtcaaaaac tggctggagc gggcgctgca
     8641 atcggaacgc aatattaaag agcagattgc cgtcctgaag ggcagcctgc tgttgtctcg
     8701 tatcctttac cagcaacaac aaacgctgcc ctcggcggat gaactggaaa acatgaccaa
     8761 ccgcatcgcg gatttgcgtc tcgaacagtt tgaagttaac cagcagcgtg atgcactctt
     8821 ccagagcgat gcgttcgtca acaaactgga agaaggtcac accaacgaag tcaacagcga
     8881 agttcacgat gcgttattgc aagtggttga tatgcgtcgc gaattgctgg atcaactcaa
     8941 caaacagttg ggtaaccagc tgatgatggc cattaacctg caaatcaacc agcagcagtt
     9001 aatgagcgta tcgaaaaacc tgaaatccat cctgactcag caaatctttt gggtgaacag
     9061 taaccgtcca atggactggg actggatcaa agcgttcccg caaagcctga aagatgaatt
     9121 taagtcgatg aaaatcacgg tgaactggga aaaagcctgg cctgccgttt ttatcgcttt
     9181 cctcgccggt ttgccgctgc tgttgattgt cgggctgatc cactggcgtc tgggctggct
     9241 gaaagcgtat caacaaaaac tggcttccgc tgtgggttcc ctgcgtaacg atagccagct
     9301 caacacacca aaagcgatcc ttatcgacct gatccgcgcg ctgccggtgt gcctgattat
     9361 tctcgcggtt ggcttgattc tgttgaccat gcagctcaac atcagcgaac tgctatggtc
     9421 gttcagcaaa aaactggcga ttttctggct ggtgtttggc ctgtgctgga aggtactgga
     9481 gaagaacggc gttgccgtgc gtcacttcgg catgccggaa cagcagacca gccactggcg
     9541 tcggcaaatt gtccgcatca gtctcgcatt gctgcctatc catttctggt ctgtggtggc
     9601 agaactttcc ccgctgcatc tgatggatga tgtgctgggg caagcgatga ttttcttcaa
     9661 cctgctgctg attgccttcc tggtatggcc aatgtgccgc gaaagctggc gtgataaaga
     9721 gtcgcacacc atgcgactgg tcaccattac cgtgctgtcg attatcccga ttgcattgat
     9781 ggtgctgact gccacgggtt atttctacac cacgctgcgc ctctccgggc gttggattga
     9841 aaccgtttat ctggtgatca tctggaactt gctctaccag acggtattgc gcggcttaag
     9901 cgtagcggcg cggcgtatcg cctggcgtcg tgcgctggcg cgtcggcaga atctggtgaa
     9961 agagggcgca gaaggtgccg aaccgccgga agaacccacc attgcactgg aacaggttaa
    10021 ccagcagacg ctgcgtatta ccatgttgct gatgtttgcg ctgttcggtg tcatgttctg
    10081 ggcaatttgg tccgatttga tcaccgtgtt cagctatatc gacagcatta cgctctggca
    10141 ttacaacggt actgaagctg gcgctgcggt ggtgaaaaac gtcaccatgg gcagtctgtt
    10201 gtttgcgatt atcgcctcaa tggtggcctg ggcgttgatt cgcaacctgc ctggtttgct
    10261 ggaagtgctg gtgctctcgc gactgaatat gcgccagggc gcgtcgtatg ccattactac
    10321 catccttaac tacatcatta ttgccgttgg tgcgatgacg gtgttcggat cgctgggcgt
    10381 ctcttgggat aaactccagt ggctggcggc agcattatcc gtaggtcttg gttttggttt
    10441 acaagaaatt ttcggtaact tcgtctccgg tttgatcatt ctgttcgaac gtccggtgcg
    10501 tattggcgat acggtaacca ttggtagctt ctcggggacg gtaagtaaga tccgtattcg
    10561 tgcgacaacg attaccgatt tcgatcgcaa agaagtgatc atcccgaaca aagcgtttgt
    10621 taccgagcgt ctgatcaact ggtcgttgac tgacactact acgcgtctgg tgatccgtct
    10681 cggcgtggcc tatggctccg atctggaaaa agtgcgtaaa gtgttactga aggcggcgac
    10741 tgagcaccca agggtgatgc acgaaccaat gccggaagtc ttctttacgg cgtttggtgc
    10801 cagcacgttg gatcatgagc tgcgtctgta tgtgcgtgaa ctgcgtgacc gtagtcgtac
    10861 tgtcgatgag ctgaaccgta ctatcgatca gctgtgccgt gaaaacgaca tcaacattgc
    10921 ctttaaccag cttgaagtgc atctgcacaa cgagaagggc gatgaggtga cggaagtaaa
    10981 acgcgactac aaaggcgatg acccgacgcc agcggtaggg taataaccaa agggggcgac
    11041 atttagttgc cccgagatta ctgacaaagt gcgcgttgtt catgccggat gcggcgtgac
    11101 cgccttatcc ggcctacgaa accgcaagaa ttcaatgtat tgcaggagcg gtgtaggcct
    11161 gataagcgta gcgcatcagg cagttttgcg tttgcccaca atcttagggg acatttagcg
    11221 gccccattta tttctcactt ttccgcctca tcatcgcgcg ttaatttctt ttcataatcg
    11281 cgttttacaa tatccagcgc gcgcagcacg gtgcgggctg ggatctgatt ttcttccagc
    11341 agcacaatca aatcgacagc cagtttgaca tcgtcagggg cattttccag tgacatactc
    11401 tctccattgc tagtgggtta agcgcgctag cctgttttcg attttttcca gcgcatggcg
    11461 gcagcgtgcc aggcgcgctt cataggcttc cacttcacga tgcagcgttt gctgttccac
    11521 gagatcggtc acccgcgcca ggcgggctct gcgttcggca accatctcac gtagccgccg
    11581 ctcaaaatcc tgatgctgaa tacgtttacg ctgccagcgg gcaattttcg gtggcgcact
    11641 gtcccactcg cgcaatgacc aggcggaggc ttcacgcgcg atggcttcca gttgtgccgc
    11701 cagatgttcc gccagccagg ccacttgcgg cagctgttgc tgctcaactg catgacgaag
    11761 cgcagccaga ttatcgccag cctcatcgag acaagcctgc agtgttgtcg tacgggtttg
    11821 aaaaaggtgt ctgtcaaaac gggcacttag cgtggcaaat tgcgctaccg gggcacaacg
    11881 ctgacgcagc gtagcgagct gtccttccag tttttccagc agcagggcgg ttttcacgat
    11941 aagacctcaa tgaaaatgat aattgttatg ctaaagtagc cactctgtaa gctgacaaca
    12001 ctcaatatgc aacgaatcat tttaatcatc attggctggc tggcggtagt gctgggtacg
    12061 ctgggcgtgg tattaccggt attaccgacg acgccgttta tcctgctggc ggcctggtgc
    12121 tttgcccgtt cttccccgcg ctttcacgcc tggttgctgt accgctcatg gtttggcagc
    12181 tatctacgtt tctggcagaa acatcatgcg atgccgcgcg gcgtcaaacc gcgggcgatt
    12241 ttgcttattt tgctcacgtt tgccatttct ctgtggttcg tccagatgcc atgggtgcgc
    12301 atcatgttgc tggtaattct cgcctgtttg cttttctata tgtggcgaat tccggtgatt
    12361 gatgaaaagc aagaaaagca ctgaagcaca acaatcgcag ttgcaattat tgcgtacagc
    12421 cagtacattc tggcgttttc gagcacaggc gcaggcggtc aaaggttaaa caactgttac
    12481 ttttgatacg tttaaaacgc gccgtgagta ccaccgtaac aagcaggcat acacttatga
    12541 ccgcgactgc acagcagctt gagtatctca aaaatagcat caaaagcatt caggactacc
    12601 caaaacccgg cattcttttc cgcgatgtca ccagcttact ggaagacccg aaagcttacg
    12661 ctctcagcat cgacttgctg gttgagcgtt acaaaaatgc gggcattacc aaagttgtcg
    12721 gcaccgaagc gcgtggcttc ttgtttggcg ctccggtagc tctgggtctg ggcgttggct
    12781 ttgtaccggt ccgtaaaccg ggcaaactgc cgcgtgaaac catcagtgaa acttacgacc
    12841 tggaatacgg caccgatcag ctggagatcc acgttgatgc catcaaaccg ggcgacaaag
    12901 ttctggtggt ggacgatctg ctggcaaccg gcggcactat cgaagcgacc gttaaactga
    12961 tccgtcgtct gggtggtgaa gtggctgacg ctgcgttcat tatcaacctg ttcgatctcg
    13021 gcggcgaaca gcgtctcgaa aaacagggca ttaccagcta cagccttgtc ccgttcccgg
    13081 gccattaatt atcgccagtc ttgtgctgcc cacgctacgg acagcacaag atgtgcattc
    13141 agcctcgccg ttctgacggg gctgtgttag cattacccct tcgtgaatcc accttccagc
    13201 gtttcagagc ctgccaatga gttatcaggt cttagcccga aaatggcgcc cacaaacctt
    13261 tgctgacgtc gtcggccagg aacatgtgct gaccgcactg gcgaacggct tgtcgttagg
    13321 gcgtattcat catgcttatc ttttttccgg tacccgtggc gtcggaaaaa cctctatcgc
    13381 ccgactgctg gcgaaggggc taaactgcga aaccggcatt accgcgacgc cgtgcggcgt
    13441 gtgcgataac tgtcgtgaaa tcgagcaggg gcgctttgtc gatctgattg aaatcgacgc
    13501 cgcctcgcgc accaaagttg aagatacccg cgacctgctg gataacgtcc agtacgctcc
    13561 ggcgcgtggt cgtttcaaag tttatctgat cgacgaagtg catatgctgt cgcgccacag
    13621 ctttaacgca ctgttaaaaa cccttgaaga gccgccggag cacgttaagt ttctgctggc
    13681 gacgaccgat ccacagaaat tgccggtgac gattttgtcg cgctgtttgc aatttcatct
    13741 caaggcgctg gatgtcgagc aaattcgcca tcaacttgag cacatcctca acgaagaaca
    13801 tatcgctcac gagccgcggg cgttgcaatt gctggcgcgc gccgctgaag gcagcctgcg
    13861 agatgcctta agtctgaccg accaggcgat tgccagcggt gacggccagg tttcaaccca
    13921 ggcagtcagt gcgatgctgg gtacgcttga tgacgatcag gcgctgtcgc tggttgaagc
    13981 gatggtcgag gccaacggcg agcgcgtaat ggcgctgatt aatgaagccg ctgcccgtgg
    14041 tatcgagtgg gaagcgttgc tggtggaaat gctcggcctg ttgcatcgta ttgcgatggt
    14101 acaactttcg cctgctgcac ttggcaacga catggccgcc attgagctgc ggatgcgtga
    14161 actggcgcgc accataccgc cgacggatat tcagctttac tatcagacgc tgttgattgg
    14221 tcgcaaagaa ttaccgtatg cgccggaccg ccgcatgggc gttgagatga cgctgctgcg
    14281 cgcgctggcg ttccatccac gtatgcctct gcctgagcca gaagtgccgc gccagtcctt
    14341 tgcacccgtc gcgccaacgg cagtaatgac gccaacccag gtgccgccgc aaccgcaatc
    14401 agcgccgcag caggcaccga ctgtaccgct cccggaaacc accagccagg tgctggcggc
    14461 gcgccagcag ttgcagcgcg tgcagggagc aaccaaagca aaaaagagtg aaccggcagc
    14521 cgctacccgc gcgcgtccgg tgccatcggc gctggaaaaa gcgccagcca aaaaagaagc
    14581 gtatcgctgg aaggcgacca ctccggtgat gcagcaaaaa gaagtggtcg ccacgccgaa
    14641 ggcgctgaaa aaagcgctgg aacatgaaaa aacgccggaa ctggcggcga agctagcggc
    14701 agaagccatt gagcgcgacc cgtgggcggc acgggtgagt caactttcgc taccaaaact
    14761 ggtcgaacag gtggccttaa atgcctggaa agaggagagc gacaacgcag tatgtctgca
    14821 tttgcgctcc tctcagcggc atttgaacaa ccgcggtgca cagcaaaaac tggctgaagc
    14881 gttgagcacg ttaaaaggtt caacggttga actgactatc gttgaagatg ataatcccgc
    14941 ggtgcgtacg ccgctggaat ggcgtcaggc gatatacgaa gaaaaacttg cgcaggcgcg
    15001 cgagtccatt attgcggata ataatattca gaccctgcgt cggttcttcg atgcggagct
    15061 ggatgaagaa agtatccgcc ccatttgatc gtaagcacag cttacgttcg tcatccttaa
    15121 cgtgattgag agagaaacct atgtttggta aaggcggtct gggtaacctg atgaagcaag
    15181 cccagcagat gcaagaaaaa atgcagaaaa tgcaggaaga gatcgcgcag ctggaagtca
    15241 ccggcgaatc tggcgcaggt ctggtaaaag tgaccatcaa cggtgcacac aactgccgtc
    15301 gcgtagagat cgacccgagc ctgctggaag acgacaaaga gatgctggaa gacctggtgg
    15361 ctgcagcatt caacgacgca gcacgtcgta ttgaagaaac gcagaaagaa aaaatggcct
    15421 ctgtatcctc cggaatgcag ctgccgcctg gctttaagat gccgttctga tgcaaaccag
    15481 cccgctgtta acacagctta tggaagcact gcgctgtctg tcgggcgttg gcccgaagtc
    15541 ggcgcagcgt atggcgttca cgctgcttca gcgcgatcgt agcggcggga tgcgtctggc
    15601 gcaggcgctc acccgggcga tgtcggaaat cggccactgc gccgattgcc gcactttcac
    15661 cgaacaggaa gtctgtaaca tctgttcgaa tccgcgtcgt caggaaaacg gtcaaatctg
    15721 tgtggtggag agtccggcgg acatctacgc cattgagcag acggggcagt tttctggtcg
    15781 ttattttgtg ttgatggggc atctgtcacc gctggacggc atcggtccgg atgatatcgg
    15841 gcttgatcgt ctggaacagc gtctggcaga ggaaaaaatc actgaagtga tcctcgccac
    15901 caatcccacg gttgaaggtg aagctaccgc taactacatt gccgagcttt gcgcgcaata
    15961 tgacgtggaa gctagccgaa tcgctcatgg cgtaccggtt ggcggcgaac tggaaatggt
    16021 cgatggcacc acgctgtcac actcccttgc cgggcgtcat aagattcgtt tttaagcaaa
    16081 cgagagcagg atcacctgct ctcgcttgaa attattctcc cttgtcccca tctctccctc
    16141 atcctgtttt taaccttaaa atggcattat tgaggtagac ctacatgaaa ggacaagaaa
    16201 ctcgtggttt tcagtcagaa gtgaaacagc ttctgcacct gatgatccat tctctctatt
    16261 ccaataaaga aatcttcctg cgtgagctta tctctaacgc ctccgatgcg gcggacaagc
    16321 tgcgtttccg tgcgctatct aacccggacc tgtacgaagg tgatggcgaa ctgcgcgttc
    16381 gtgtctcttt cgataaagac aagcgcacgc tgaccatctc cgataacggc gtggggatga
    16441 cccgcgacga agtgattgac catctgggga ctatcgctaa atccggtacc aaatcattcc
    16501 tcgaatccct gggttctgac caggcgaaag acagccagct gatcggtcag tttggtgttg
    16561 gtttctactc tgcgtttatc gtggccgaca aagtgactgt gcgtactcgc gcggcaggcg
    16621 aaaaaccaga aaacggcgtc ttctgggaat cggctggcga aggtgaatat accgttgccg
    16681 acatcaccaa agaagatcgt ggtactgaaa tcaccctgca tctgcgtgaa ggcgaagacg
    16741 agttcctcga tgactggcgc gtgcgttcca tcatcagcaa atactccgac catatcgcgc
    16801 tgccggtaga gatcgaaaaa cgcgaagaga aagacggcga aaccgttatc tcctgggaga
    16861 aaatcaacaa agcgcaggcg ctgtggactc gtaacaagtc ggaaatcacc gatgaagagt
    16921 acaaagagtt ctacaaacac atcgcccacg actttaatga tccgctgacc tggagccaca
    16981 accgcgttga aggtaagcag gagtacacca gcctgctgta catcccgtcc caggctccgt
    17041 gggatatgtg gaaccgtgat cataaacacg gcctgaaact gtacgtccag cgtgtgttca
    17101 tcatggacga cgcagaacag ttcatgccga actatctgcg cttcgtgcgt ggtctgattg
    17161 actccagcga tctgccgctg aacgtttccc gtgaaatcct ccaggacagc acggtaacgc
    17221 gtaacctgcg caatgcgctg accaagcgtg tgctgcaaat gctggaaaaa ctggcgaaag
    17281 acgacgcgga aaaataccag accttctggc aacagtttgg cctggtactg aaagaaggtc
    17341 cggcggaaga tttcgctaac caggaagcga tcgccaaact gctgcgtttt gcttctaccc
    17401 ataccgattc ttctgcgcag accgtatctc tggaagacta cgtttcccgc atgaaagaag
    17461 ggcaggagaa aatctactac atcaccgcag acagctatgc ggcagcgaag agcagcccgc
    17521 acctggaact gctgcgtaag aaaggcatcg aagttctgct gctttccgac cgcatcgatg
    17581 agtggatgat gaactatctg actgagttcg acggtaaacc gttccagtcg gtgtctaaag
    17641 ttgacgagtc gctggaaaaa ctggctgacg aagttgatga gagcgcgaaa gaagcggaga
    17701 aagcactgac tccgttcatc gaccgagtga aagccttgct cggcgagcgc gtgaaagatg
    17761 tccgtctgac tcaccgtctg accgatacgc cagcgatcgt ttcgaccgac gcggacgaaa
    17821 tgagcactca gatggcgaaa ctgttcgctg cggcgggcca gaaagtgcca gaagtgaaat
    17881 acatcttcga actgaacccg gatcacgtac tggtgaaacg tgcggcagat actgaagatg
    17941 aagcgaagtt cagcgagtgg gtagaactgc tgctggatca ggcgctgctg gcagaacgcg
    18001 gcacgctgga agatccgaac ctgtttattc gtcgtatgaa ccagctgctg gtttcctgat
    18061 gtcatgccgg atgacctttg tgtcatccgg catttttctt ttcatcatct gcactttccg
    18121 caaattatct cgccattaac cgtttcagcc tcaggtgcct ttcttgaggc aatcgcctgt
    18181 tggtggtatc gtttatcgct ttttcaaaaa attcgacaca ttttaagggg attttcgcaa
    18241 tgcgtatcat tctgcttggc gctccgggcg cggggaaagg gactcaggct cagttcatca
    18301 tggagaaata tggtattccg caaatctcca ctggcgatat gctgcgtgct gcggtcaaat
    18361 ctggctccga gctgggtaaa caagcaaaag acattatgga tgctggcaaa ctggtcaccg
    18421 acgaactggt gatcgcgctg gttaaagagc gcattgctca ggaagactgc cgtaatggtt
    18481 tcctgttgga cggcttcccg cgtaccattc cgcaggcaga cgcgatgaaa gaagcgggca
    18541 tcaatgttga ttacgttctg gaattcgacg taccggacga actgattgtt gatcgtatcg
    18601 taggccgccg cgttcatgcg ccgtctggtc gtgtttatca cgttaaattc aatccgccga
    18661 aagtagaagg caaagacgac gttaccggtg aagaactgac tacccgtaaa gacgatcagg
    18721 aagaaaccgt acgtaaacgt ctggttgaat accatcagat gactgcaccg ctgatcggct
    18781 actactccaa agaagcggaa gcgggtaaca ccaaatacgc gaaagttgac ggcaccaagc
    18841 cggttgctga agttcgcgct gatctggaaa aaatcctcgg ctaatcaagc cgcagcagat
    18901 ctgctttcgg gcaggtctgc tcatccacct caggcaatac ctctcacagc aattagttct
    18961 tcttcctcac ttttccgcta caattatcaa caagttgaat cgataagagg cggtaatgcg
    19021 tcagactaaa accggtatcc tgctggcaaa cctgggtacg cccgatgccc ccacacctga
    19081 agcggtaaaa cgctatctga aacaattttt aagcgacaga cgcgtggttg atacctcacg
    19141 gttgttatgg tggccattgc tgcgcggcgt tattttgccg ctacgctcgc cgcgtgtggc
    19201 gaagctgtat gcctctgtct ggatggaagg tggctcgccg ctgatggttt acagccgcca
    19261 gcaacagcag gcgctggcac aacgtttacc ggagacgcct gtagcgctgg gaatgagcta
    19321 cggctcgcca tcactggaaa gcgccgtcga tgaactcctg gcagagcatg tagatcatat
    19381 tgtggtgctg ccgctttatc cgcaattctc ctgttctacg gtcggtgcgg tatgggatga
    19441 actggcacgc attctggcgc gcaaacgtag cattccgggg atatcgttta ttcgtgatta
    19501 cgccgataac cacgattaca ttaatgcact ggcgaacagc gtacgcgctt cttttgccaa
    19561 acatggcgaa ccggatctgc tgctgctctc ttatcatggc attccccagc gttatgcaga
    19621 tgaaggcgat gattacccgc aacgttgccg cacaacgact cgcgaactgg cttccgcact
    19681 ggggatggca ccggaaaaag tgatgatgac ctttcagtcg cgctttggcc gggaaccctg
    19741 gctgatgcct tataccgacg aaacgctgaa aatgctcgga gaaaaaggcg taggtcatat
    19801 acaggtgatg tgcccgggct ttgctgcgga ttgtctggag acgctggaag agattgccga
    19861 gcaaaaccgt gaggtcttcc tcggtgccgg cgggaaaaaa tatgaatata ttccagcgct
    19921 taatgccacg ccagaatata tcgaaatgat ggctaatctt gttgccgcgt atcgctaaag
    19981 ctgagcggta aagaactgag cgccgtcgcg aagagcctcg tcggcggttt tcatcattcg
    20041 tgaataatgc aaaaaggcat gcagcgtacc tgggtagagt ttgaactcac agggctgctg
    20101 atgcgccgct aacgtctggt aaagcagacg gctgtcatcc agcagcggat cgaactccgc
    20161 cccggcaata aaacagggcg gaacttcgcg agtgagatca ttattaaaca gacagtaata
    20221 cggtgactcg cggtccgcgt cgttgcttaa atacgcctct tcatacatct gcaaatcctg
    20281 ttgcgttaag ccatcccaga caccgcccaa cagacgacga gtcacggaat cccgtaatcc
    20341 gtaaagccca taccacagca aaacacccac aattttaccg caatcgatct gtttatcacg
    20401 caaccacaac gcactggcga gcgccagcat ggcacctgcg gaatcaccgg caaagccaat
    20461 gcgggacata ttgatttgat aatcctccgc ctgctggtgg aaataacaac aagcagccac
    20521 aatttcctct atcgcttgcg gaaaacgcgc ttcaggtgaa agggggtaat caataccaat
    20581 caccgtacat tggctgtagc ttgccagcag gcgcatgatg cgatcgtggg tatcgagatt
    20641 gccgagaata aaaccgcctc catgcaaata aaatagcgtc gccgggctat ctggctgcgg
    20701 acaaaagaga cgtgtttcca cctgcccata ttttgttgga accatgtaag ctctggttgc
    20761 catttctgga gcgcccgcat tccagaatcg gcgctcaagc gtgtaatact gtcgttgctc
    20821 agcaatcgtt cccgttgcgg gccaggacgg taaatccggc tgaagagtat tcacaacggt
    20881 cttcatttca gcagaaataa ggtccagaac aggtagtttg ttttccggct tcatacaaaa
    20941 actcctttca aattacgtca ttgtaaggaa ccactgccat gaaaatgcga tcccgcctgc
    21001 tgatattgaa actggctgcg tctcgcgcgc tcccgtcaga ttgtgttaac attcgccgct
    21061 cagttaacca cccgtaaaaa caaccatgaa atttcccggt aaacgtaaat ccaaacatta
    21121 cttccctgta aatgcacgcg atccgctgct tcagcaattc cagccagaaa acgaaaccag
    21181 cgccgcctgg gtcgtaggta ttgaccagac gctggtcgat attgaagcga aagtggatga
    21241 tgaattcatt gagcgttatg gattaagcgc cgggcattca ctggtgattg aggatgacgt
    21301 agccgaagcg ctttatcagg agctaaaaca gaaaaacctg attacccatc agtttgcggg
    21361 tggcaccatt ggtaacacca tgcacaacta ctcggtgctc gcggacgacc gttcggtgct
    21421 gctgggcgtc atgtgcagca atattgaaat tggcagttat gcctatcgtt acctgtgtaa
    21481 cacttccagc cgtaccgatc ttaactatct acaaggcgtg gatggcccga ttggtcgttg
    21541 ctttacgctg attggcgagt ccggggaacg tacctttgct atcagtccag gccacatgaa
    21601 ccagctgcgg gctgaaagca ttccggaaga tgtgattgcc ggagcctcgg cactggttct
    21661 cacctcttat ctggtgcgtt gcaaaccggg tgaacccatg ccggaagcaa ccatgaaagc
    21721 cattgagtac gcgaagaaat ataacgtacc ggtggtgctg acgctgggca ccaagtttgt
    21781 cattgccgag aatccgcagt ggtggcagca attcctcaaa gatcacgtct ctatccttgc
    21841 gatgaacgaa gatgaagccg aagcgttgac cggagaaagc gatccgttgt tggcatctga
    21901 caaggcgcta gactgggtag atctggtgct gtgcaccgcc gggccaatcg gcttgtatat
    21961 ggcgggcttt accgaagacg aagcgaaacg taaaacccag catccgctgc tgccgggcgc
    22021 tatagcggaa ttcaaccagt atgagtttag ccgcgccatg cgccacaagg attgccagaa
    22081 tccgctgcgt gtatattcgc acattgcgcc gtacatgggc gggccggaaa aaatcatgaa
    22141 cactaatgga gcgggggatg gcgcattggc agcgttgctg catgacatta ccgccaacag
    22201 ctaccatcgt agcaacgtac caaactccag caaacataaa ttcacctggt taacttattc
    22261 atcgttagcg caggtgtgta aatatgctaa ccgtgtgagc tatcaggtac tgaaccagca
    22321 ttcacctcgt ttaacgcgcg gcttgccgga gcgtgaagac agcctggaag agtcttactg
    22381 ggatcgttaa gttatcgtcg gttcgtaggc cagataaggc gttcacgccg catctggcat
    22441 ttggctctcg atgcctgatg cgacgctggc gcgtcttatc atgcctacat atttttcata
    22501 ttttacatcc ggcaaccacc gtttaccccg tcaccacctc acccgccggt ggcgtttcca
    22561 gcagttccag catggtacgg gcgatttcac gctcgcccat cactacctga ttcgcaccac
    22621 gttcggtgat atacgccact tcatcgtcat aatgggcgcg ggcaataatc tcaatatccg
    22681 gatttttcgc gcgggcagat gccacaatct cacccgcttc ataaccgttg ggaatcgtca
    22741 ggatcagcca ttttgcacat tccagatgcg ccagttgcat aatttcttcg ttcgccgcat
    22801 tgcccaatac tgcgcggacc ccgcgctctc gcagctcatc aacacgggtt cgtgacgtct
    22861 caatcaccac cagcggaata tcagaggcga gcaatttctc ccccagcagg ctgcctacac
    22921 gaccgtaacc caccagtagc gcatggttgc aaatatccac tgggatctgc ttctcttctt
    22981 cgattgcctc ttccagcgtc tgctcttcca gcgtttcggt cttcgccaga tatttctcca
    23041 gtagtgcgaa cagtaccggg ttgagcataa tcgacaggat cgcccctgcc agtaccaggt
    23101 tttgtccggc ctgcggcagt aaattcaatg ccattcccag tcccgccagg ataaacgcga
    23161 actcaccaat ctgcgccagg ctggcggcga tggttaatgc cgtacgttgg gagtgaccaa
    23221 acagtcgcac caggaaaaat gcggctaacg acttaccaaa cagaataatc gccagcgtcg
    23281 ccagcactgc cagcggttgc tgaatcagaa ttaacggatc aaacaacatc ccgacggaga
    23341 caaaaaacag caccgcaaac gcgtcgcgca atggcagcgt atcgtgggcg gcacggtgac
    23401 tcagttcaga ctcgttcagt accatcccgg caaagaacgc accgagtgca aaggagacat
    23461 caaacagctc taccgcacca aaggcaatcc ctaacgccag cgccagcacc gacagggtaa
    23521 acagctcgcg agaaccggtt gccgcgctgc gtgccataat ccacggcacc agacggcgac
    23581 ctaccagcat cataatggcg ataaatgcga tcactttgcc gatggtgatc cccatatcga
    23641 ctgcaagagt ggcaaagccc acatcgccct gttccatcat tcctgccact gcgggcagca
    23701 acaccagcgt cagaaccatt accaggtctt ccacaatcaa ccaaccgatg gcgatttgcc
    23761 cacgctgact gtcaattaat tgccgttctt caagtgcgcg cagtaacacc acggtactgg
    23821 cggtggaaag acataaaccg aacacgatac cggtcattaa cgaccagccc agcacggcag
    23881 agagcgccat acccagcagc gtcgccacgg ctatctgggc gatcgcaccg ggaatggcga
    23941 tggcctttac cgccatcaaa tccttcagcg aaaagtgcaa accgacgcca aacatcaaca
    24001 gaatgacgcc cagttcagcc agttccggcg caagcttggt atcggcaaca aagcccggag
    24061 tgaatggtcc tgccagcaca cccgctaaca gatatcccac cagaggagaa atacgtagtt
    24121 tattggccag catgccgagg ataaaggcga gcacaaggcc gccaacaatg gtggtgataa
    24181 gcggggtggc gtgatgcatt ccgtctcctt ttcctggtgg ttattgtcca tttttggccg
    24241 ggaaaaccaa aattacaggt aatagtttat gacaatttca ttgatgatgt tcatgaataa
    24301 ttattgaatt ttgcagaaaa atggaattag ctgcaaaaaa agcacggatc ggaaaacaga
    24361 aggcggttta acagaggaaa ggcttatggc gactgtaaaa cgatgcagcc aaagtttgcc
    24421 tttggctgca atgaaatcag tctttatgcc ggttatcagg caggaatatg gtcaacatcc
    24481 ccaatagtgg caggaaagca cagattttat agactaactc gatgctggtg tgatcggcga
    24541 taagccccag aactgccgct cccagacctc ccatgccaaa agcaaaaccg aaaaagagtc
    24601 cagaaaccat accgatacgt cccggaagta gctcctgagc gtagaccaga atggcagaga
    24661 atgccgaagc gaggataaat ccaataatca ccgttaaaac ccccgtccag tgcaggctgg
    24721 cgtagggtaa aatcagcgta aacggcgcaa cgccgaggat agagccccaa atcacatatt
    24781 tccgtccaat tttatcccct acaggcccgc cgatcaccgt acctgccgca acggcaaaca
    24841 ggaaggcaaa cagatgaagc tgggcattct ggatagataa tccgaatttt tgcatcagat
    24901 aaaaggtgta atagctg
//
LOCUS       DADLFA010000045        24732 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000045 DADLFA010000000
VERSION     DADLFA010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24732
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.045"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..88
                     /locus_tag="JQH65_001541"
     CDS             <1..88
                     /locus_tag="JQH65_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HAY6139003.1"
                     /translation="PLINKEEQPDKGFLASISRGIMPSLPFL"
     gene            complement(144..2999)
                     /gene="valS"
                     /locus_tag="JQH65_001542"
     CDS             complement(144..2999)
                     /gene="valS"
                     /locus_tag="JQH65_001542"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAY6139004.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(2999..3442)
                     /gene="holC"
                     /locus_tag="JQH65_001543"
     CDS             complement(2999..3442)
                     /gene="holC"
                     /locus_tag="JQH65_001543"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HAY6139005.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            complement(3602..5113)
                     /gene="pepA"
                     /locus_tag="JQH65_001544"
     CDS             complement(3602..5113)
                     /gene="pepA"
                     /locus_tag="JQH65_001544"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HAY6139006.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAILHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            5380..6480
                     /gene="lptF"
                     /locus_tag="JQH65_001545"
     CDS             5380..6480
                     /gene="lptF"
                     /locus_tag="JQH65_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HAY6139007.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6480..7562
                     /gene="lptG"
                     /locus_tag="JQH65_001546"
     CDS             6480..7562
                     /gene="lptG"
                     /locus_tag="JQH65_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HAY6139008.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQTFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(7723..9225)
                     /locus_tag="JQH65_001547"
     CDS             complement(7723..9225)
                     /locus_tag="JQH65_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HAY6139009.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEKQGAAHFFGEPMLDIKDWMRTDTNGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGFQASTKQQNNPPAKGKAVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(9355..10374)
                     /gene="ahr"
                     /locus_tag="JQH65_001548"
     CDS             complement(9355..10374)
                     /gene="ahr"
                     /locus_tag="JQH65_001548"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HAY6139010.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELRPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIKHVRDGKARYRVV
                     LKADF"
     gene            10570..10654
                     /locus_tag="JQH65_001549"
     tRNA            10570..10654
                     /locus_tag="JQH65_001549"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10604..10606,aa:Leu,seq:caa)
     gene            10818..12082
                     /locus_tag="JQH65_001550"
                     /pseudo
     CDS             10818..12082
                     /locus_tag="JQH65_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522431.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
     gene            complement(12137..13147)
                     /locus_tag="JQH65_001551"
     CDS             complement(12137..13147)
                     /locus_tag="JQH65_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6139011.1"
                     /translation="MIYITSESKETLMIKYEGKMKKNIRVIPKNIHSKLRRLGNTVVA
                     GTSIAFTENQLKSGALEHLGIYFDNGVNAYETSVIPDPLQGKYSLKNVFGEEIVRKDL
                     PKETHYTEIESPNWGDSSNGTHTVRLPYEKYPRDIIPPTLIAIEINHKQPSDSHFIFN
                     FRVTRILDKQSNKFDDELLFDLNLLQENLGKCGIEDADKPISTYADTLIVSWEIFPPG
                     SKEETLARIFRGKNITSDKKNVAENRYDFFMSLEPKKIVTGNSTFSNYIGAMLEDDLV
                     VFENIEYGNAIYILYDNWDDISKLSRIDLLSGRAGSNFDRIIHSGNWKDEVRKKVAAG
                     RL"
     gene            complement(13098..13634)
                     /locus_tag="JQH65_001552"
     CDS             complement(13098..13634)
                     /locus_tag="JQH65_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6139012.1"
                     /translation="MLKAIAGIAKYTWIYMAIFIISLMIISSSDINYVYNDFANYCTL
                     LVSVSGMVFTIMGIWLAFLYPNALQRIVNPKTIETVDFTEQLEDTRRLEAIVGSVLKS
                     SFVMITLLVVYLVKLVFFKSHLYLESQDVIKNTSLALIIAISYMQIEAVISVILANVM
                     FINDLHNKREQRDIDDQV"
     gene            complement(13635..14714)
                     /locus_tag="JQH65_001553"
     CDS             complement(13635..14714)
                     /locus_tag="JQH65_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6139013.1"
                     /translation="MNVGHLNFFKVNKCGLYKVNDDNTYGLELSETFDLIQDWVGTKS
                     LALTIPWDPKEKPNSSKCYCKDIYKDENTGDFLVMLWKSDTDSTGSLLGASEDGEIGS
                     SSVVKYTNSYRGKKVIWGRPCFYWVIPELETIVSIKFDHSVCDSELFQDYVHSSITNR
                     VKHSKRVKNKTEKGYIRLSNTDDEDLYKYMYRFDMKLRSLETTHTELGKLIPKITHIV
                     RRETIIINPNDARADWLKTFSTLVPFVSGKKNAKTRQIEIKAEAKPSLNEVQEIIEKY
                     SAEDREKRLWDNVGFATDKGITWVDKYRMRDILNVPSDDYSTYSAAYIYEQISHKRKE
                     FISPILKELKALQSQPRIKKASGED"
     gene            complement(15112..15684)
                     /locus_tag="JQH65_001554"
     CDS             complement(15112..15684)
                     /locus_tag="JQH65_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Polarity suppression protein"
                     /protein_id="HAY6139014.1"
                     /translation="MESTALQQVFDTCQNNKAAWLQRKNELAAAEQEYLRLLSGEGRN
                     VSRLDELRNIIEVRKWQVNQAAGRYIRSHEAVQHISIRDRLNDFMQQHGTALAAALAP
                     ELMGYSELTAIARNCAIQRATDALREALLSWLAKGEKINYSAQDSDILTTIGFRPDAT
                     SVDDSREKFTPAQNMIFSRKSAQLASRQSV"
     gene            complement(15758..16258)
                     /locus_tag="JQH65_001555"
     CDS             complement(15758..16258)
                     /locus_tag="JQH65_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transactivation protein"
                     /protein_id="HAY6139015.1"
                     /translation="MIYCPSCGHVAHTRRAHFMDDGTKIMIAQCRNIYCSATFEASES
                     FFSDCKDSGMEYISGKQRYRDSLTSASGSMKRPKRMLVTGYCCRRCKGLALSRTSRRL
                     SQEVTERFYVCTDPGCGLVFKTLQTINRFIVRPVTPDELAESLHEKQELPPVRLKTQS
                     YSLRLE"
     gene            complement(16255..16989)
                     /locus_tag="JQH65_001556"
     CDS             complement(16255..16989)
                     /locus_tag="JQH65_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoprotein 3"
                     /protein_id="HAY6139016.1"
                     /translation="MSDNTIPEYLQPALAQLEKARAAHLENARLMDETVTAIERAEQE
                     KNALAQADGNDADDWRTAFRAAGGVLSDELKQRHIERVARRELVQEYDNLAVVLNFER
                     ERLKGACDSTATAYRKAHHHLLSLYAEHELEHALNETCEALVRAMHLSILVQENPLAN
                     TTGHQGYVAPEKAVMQQVKSSLEQKIKQMQISLTGEPVLRLTGLSAATLPHMDYEVAG
                     TPAQRKVWQDKIDQQGAELKARGLLS"
     gene            17541..17807
                     /locus_tag="JQH65_001557"
     CDS             17541..17807
                     /locus_tag="JQH65_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HAY6139017.1"
                     /translation="MQAVFSSPSPAPVTPLMPLPDITQERFLRLPEVMHLCGLSRSTI
                     YELIRKGEFPPQVSLGGKNVAWLHSEVTAWMAGRIAGRKRGYDA"
     gene            17804..18403
                     /locus_tag="JQH65_001558"
     CDS             17804..18403
                     /locus_tag="JQH65_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000980235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HAY6139018.1"
                     /translation="MMMPALQKLPFSGLPFSGICGYSFSAAAKSAAGRENPSNSQATY
                     DAPRVFFCVAINATERQIMVWCVVSRADGIPCILPASAHYAAESMVAQAGQPPGWPVS
                     CEAGILTPVWAIAIERENSGDSVICYSQEAAIMATTLTPSHPEFVFVFAAVRRADRHP
                     RICMLRTVAGDERSARRSLVRDYVLSLAARLPVVEVSRA"
     gene            18396..18683
                     /locus_tag="JQH65_001559"
     CDS             18396..18683
                     /locus_tag="JQH65_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Derepression protein"
                     /protein_id="HAY6139019.1"
                     /translation="MRNKKAPQTVSARHDAREHLSIEAYHKLNRASAVSQFVGGDLIH
                     RELSGLHQLYIPHIFSYLNEDIDFVLNELKAKGLCRDFLAQQKDRGDRTHV"
     gene            18676..19131
                     /locus_tag="JQH65_001560"
     CDS             18676..19131
                     /locus_tag="JQH65_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016569899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6139020.1"
                     /translation="MFDFPQPGEIYRSAGFPDVAVVGILEDGIPWEMPYRCPDIVWNP
                     YRRKFSILVRILADGRTTDIPLGRFLREFTCDRPDLFKRSPVNRHAVLKEMAGDPELQ
                     KWREKYLDIYPQDTVPVSRAAPVAREWREIPRTEPDPEITPDNSYRNYL"
     gene            19267..19587
                     /locus_tag="JQH65_001561"
     CDS             19267..19587
                     /locus_tag="JQH65_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 domain-containing protein"
                     /protein_id="HAY6139021.1"
                     /translation="MKTPLPPVLRAALYRRAVACAWLTVCERQHRYPHLTLESLEAAI
                     AAELEGFYLRQHGEEKGRQIACALLEDLMESGPLKAAPSLSFLGLVVMDELCARHIKA
                     PVLH"
     gene            19602..21935
                     /locus_tag="JQH65_001562"
     CDS             19602..21935
                     /locus_tag="JQH65_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAY6139022.1"
                     /translation="MKMNVTATVSHALGHWPRILPALGIQVLKNHHQPCPVCGGSDRF
                     RFDDREGRGTWYCNQCGAGDGLKLVEKVFGVSPSDVAAKVAAVTGSLPPADPAVTAAA
                     VAETDAARKNAAALAQTLMAKTRPGTGNAYLTRKGFPGRECRMLTGTHRAGGVSWRAG
                     DLVVPLYDDSGELVNLQLISADGRKRTLKGGQVRGTCHTLEGQNQAGKRLWIAEGYAT
                     ALTVHHLTGETVMVALSSVNLLSLASLARQKHPACQIVLAADRDLSGDGQKKAAAAAD
                     ACEGVVALPPVFGDWNDAFTQYGGEVTRKAIYDAIRPPAESPFDTMSEAEFSAMSTSE
                     KAMRIYEHYGEALAVDANGQLLSRYENGVWKVLPPLDFARDVAGLFQRLRAPFSSGKV
                     ASVVDTLKLIIPQQEAPSRRLIGFRNGVLDTQNGTFHPHSPSHWMRTLCDVDFTPPVE
                     GETLETHAPAFWRWLDRAAGGRAEKRDVILAALFMVLANRYDWQLFLEVTGPGGSGKS
                     IMAEIATLLAGEDNATSATIETLESPRERAALTGFSLIRLPDQEKWSGDGAGLKAITG
                     GDAVSVDPKYRDAYSTHIPAVILAVNNNPMRFTDRSGGVSRRRVIIHFPEQIAPQERD
                     PQLKDKITRELAVIVRHLMQKFSDPMLARSLLQSQQNSDEALNIKRDADPTFDFIGYL
                     ETLPQTSGMYMGNASIIPRNYRKYLYHAYLAYMEANGYRNVLSLKMFGLGLPVMLKEY
                     GLNYEKRHTKQGIQTNLTLKEESYGDWLPKCDDPATT"
     gene            22282..22476
                     /locus_tag="JQH65_001563"
     CDS             22282..22476
                     /locus_tag="JQH65_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAY6139023.1"
                     /translation="MWIKDALVWIRATDPNLLIKNYLTFRFIDGLKIDWSEKQWGHKR
                     GHISLLCFIICFLSLTYFDI"
     gene            22694..23962
                     /locus_tag="JQH65_001564"
     CDS             22694..23962
                     /locus_tag="JQH65_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HAY6139024.1"
                     /translation="MALTDTKVRSAKPEEKEYSLVDGDGMSLLVKPGGSKYWRFRFRF
                     GGKQHLMAFGVYPDVSLADARKKREEARKLVAAGIDPREHKRAVKEEQAKEIITFEKV
                     AREWLVTNQKWSEEHANRVKKSLEDNIFPAIGSHNIAELGTRDLLIPIKAVEKSGRLE
                     VASRLQQRTTAIMRYAVQSGLIDYNPAQEMAGAVASSNRQHRPALELKRIPELLQKID
                     EYTGRPLTRWATELTLLIFIRSSELRFARWSEIDFETSMWTIPPEREPIPGVKHSQRG
                     AKMRTPHLVPLSKQALAILKQIKQFCGEHELIFIGDHDPSKPMSENTVNSALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSKDAVERQMSHMERNSVRAAYIHKAEHLDERKLM
                     LQWWADFLDANREKGITPFDYAKINRGNGE"
     gene            24353..>24732
                     /locus_tag="JQH65_001565"
     CDS             24353..>24732
                     /locus_tag="JQH65_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6139025.1"
                     /translation="MNFKKKLEEHFKQFEASPVLFVGSGVSRRYLGVPCWQDLLKHFA
                     EAIGENHIKLKTKSNGDLPEYAQLLVSAYAEKWWDTEEGQLALSEKEQEKTFINEQSP
                     LKLSISKYIENAHKNIIDNDELKH"
BASE COUNT         5890 a         6405 c         6135 g         6302 t
ORIGIN      
        1 tcctttaatc aataaagaag aacaaccaga taaaggcttc ttagccagta tttcacgggg
       61 aattatgcct tctttaccat ttctgtaaga gataaaaaag gccggagcat gctccggcct
      121 tcgttttcat cactgtgttt tgattacagc gcggcgataa cagcctgctg ttcaatcagc
      181 ttcgctttcg cttctgcata gccttccagc ttctcacgct ctttcgcgat gaccgcttcc
      241 ggtgcgcggg cgacaaagcc ttcattcgcc agcttgttct cgatacggct gatttcgcct
      301 tcgatcttcg ccacttcttt cgccagacgc gccagctcat cttctttgtt gatgaggcca
      361 gccatcggga tcagcagctc tgcgccgtcg atgatcttcg taacggaaac cggacctttg
      421 tcatcggcag gcagcacggt gatgctttcc agacgcgcca gggtttgcag gaagccacgg
      481 ttttcattta cgcgacgttc tgcatccgcg ctgcaaccac gcagcagcag ctccagcggt
      541 ttgcccggcg cgatgttcat ttctgcacgg atgttacgta ccgcaacgat cgcctgtttc
      601 agccactcgg tgtcagccag tgcggcttca tcaacctgag atgcatcgta ctgcgggaac
      661 ggctgcagca tgatggtgtc ggcagtgata ccgcaaagta ctttcacacg ctgccagatg
      721 gtttcggtga tgaacggaat gatcggatgc gcgaggcgca gcagaccttc cagtacagtc
      781 accagcgtat ggcgagtacc gcgcagttcg gcttcggttc caccgttcat taccggctta
      841 gtcagctcca gataccagtc gcagaactgg ttccaggtga actcatacag aatgcctgca
      901 gcgatatcga agcggaagct gtccagcgct tcgcggtacg ctttgatggt ctggttgaac
      961 tctgccagaa tccagcggtc cgccagcgac agcgtcattt caccaccgtt gaagccgcaa
     1021 tcctgacctt ccgtgttcat cagcacaaag cggctggcgt tccacagctt gttacagaag
     1081 ttacggtagc cttccaggcg tttcatatcc cagttgatgt cacgaccggt agaagccagc
     1141 gccgccaggg tgaagcgcag ggcatcggtg ccgtgcggct caatgccgtt cgggaactgt
     1201 ttctcggtgc gcttacggat tttgtccgcc agctgcggct gcatcatatt gccggtacgt
     1261 ttctccagca gttccggcag cgaaataccg tcaaccatat ccagcgggtc aataacgtta
     1321 cccttggatt tggacatctt ctggccttcg tcgtcacgaa tcaggccggt catgtaaacg
     1381 gtgtggaacg gcacctgcgg tttgccattt tcatctttga tgaagtgcat ggtcatcatg
     1441 atcatgcggg caatccagaa gaaaatgatg tcgaaaccag ataccatcac gctggttggg
     1501 tggaactggc gcagtgcgtc ggtgttttcc ggccagccta gggtagagaa ggtccacagc
     1561 gcagaagaga accaggtgtc gagaacgtct tcgtcctgac gcagggcaac gtcggcaccg
     1621 aggttatttt ccttgcgcac ttcttcttcg ttgcggccaa cataaacgtt acctgcttcg
     1681 tcgtaccatg cagggatacg gtgaccccac cacagctgac gagagataca ccagtcctga
     1741 atatcgcgca tccaggagaa gtacatgttt tcgtactgct tcggtacgaa ctgaatgtcg
     1801 ccgttctcaa ccgcctcaac cgccggtttc gccagtacat cggcacgcac gtaccactgg
     1861 tcggtgagca tcggttcgat aactacgccg ccacggtcgc cgtaaggcac ggtcaggtcg
     1921 tgcggtttaa tttcttccag caggccgagc gcgtcaaccg cagcaacgac tgctttacgt
     1981 gcagcaaaac gctccagttt ctggaactct gccgggattt cgctggaata aacgtcagat
     2041 tcgttacctt tggtatcgaa cacctgggcg ctttcacgga tatcgccgtc aaaggtcagg
     2101 atgttgatca tcggcagcgc gtgacgttta cccacttcat agtcgttaaa gtcgtgcgcc
     2161 ggagtgattt tcacgcagcc ggtgcctttt tccatgtcgg cgtgttcgtc gccaacgatc
     2221 ggaatacgac ggttaaccag cggtagaatg acatatttgc caatcaggtc tttgtaacgc
     2281 ggatcttccg ggttaacggc cacgccggta tcgcccaaca gggtttccgg acgggtagtc
     2341 gcaaccacca gataatcttt accgtctgcg gttttcgcgc cgtcagccag cggatagcgg
     2401 atgtgccaca tcgaaccttt cgattcgcgg ttttccactt ccaggtcaga gatagcggtg
     2461 cgcagtttcg gatcccagtt taccaggcgt ttgccacggt aaatcaggtc ttctttatac
     2521 agacgaacga aaacttcttt caccgcattg gacaggcctt cgtccatcgt gaagcgttca
     2581 cgctcccagt cgacggagtt gccgagacgg cgcatctgac gggtaatcgt gccgccggat
     2641 tccgctttcc attcccaaat tttgtcgatg aaagcttcgc ggccgtagtc gtgacgggtt
     2701 ttaccttctt ctgcggcaat cttgcgctca acgaccatct gggtagcgat cccggcgtgg
     2761 tcagtaccga cctgccacag ggtgtttttg ccctgcatgc gctgatagcg gatcatggta
     2821 tccatgatgg tttgctggaa ggcgtgaccc atatgcaaac tgccggtgac gttcggcggc
     2881 gggatcatga tgcagaaact ttcctggctt tcatcgccat taggcttaaa gtagccctgc
     2941 ttttcccagt gctcgtaaag cggctgttcg atatcttgtg ggttatatgt cttttccatt
     3001 atttccaggt tgccgtattc aggttgaaac cagccacgcg gtaggcttta tagcgttcgc
     3061 gcgccagttg tttcagagaa tcttcataag gaacgaagtc taccacttct gtgaaagcgg
     3121 tggcaaaatc tgcaaagctt gttcgcagac taatcaatat atcgcgcggg ctgctgctgc
     3181 gtttttgcgg ccaggcgatc tccaccggcg caccgccgcg cggtccttct cccgctaaat
     3241 tatgcggaac aaagctttct gccggacgcg cccacagggc ttcatccagc cggtaagcct
     3301 gcttttcatc ttcacaggcg atgagcacgc gcttaccgct gcgccaacgt tctgcggcaa
     3361 tttcacacac cagttgctca acggcgctta agccatcgac ggtggtgtca ttgtccagaa
     3421 ggtagaacgt cgcgtttttc atatatgggg cttcttgtgg tggatttaaa tacgcatcag
     3481 gtatacaccg cagcgcctga tgcgacgctg ttgtgtctta tcaggcctac gagttcagtg
     3541 ctgtgtaggt cggataaggc gttcacgccg catccggcaa taacagcctt gcctgacgca
     3601 attactcttc gccgttaaac ccagcgcggt ttaacaggaa ctgtgccagc aacgccaccg
     3661 gacgaccggt ggcgcctttt gctttaccag aacgccaggc ggttccggcg atatctaggt
     3721 gcgcccagtt gtacttacgg gtaaagcgcg acaggaagca acctgcggta atcgccccac
     3781 caggacgacc gccaatgttc gccatatcgg caaaattgga ctccagctgt tcctgatact
     3841 cgtcacccag cggtaagcgc catgcgcggt caccggattg ttcagacgcg gcaatcagtt
     3901 catgggccag cggattatgg ttcgccatca gaccggtaat gtgatgaccc agcgcgatca
     3961 cgcaggcacc ggtcagcgtc gccacgtcaa tcaccgcttc cggctcaaaa cgctcaacgt
     4021 aagttaacac gtcgcacagt accaggcggc cttcagcgtc ggtattcagc acttcaacag
     4081 tttgaccgga catggtggtt aacacatcgc ccggacgata ggctcgtccg ccaggcatgt
     4141 tttcgcagcc tgccaacacg ccgataacgt taatcggcag ttgtagctcc gcgaccatcc
     4201 gcatcacgcc gtaaaccgct gccgcaccgc acatatcgta cttcatctca tccatgcctt
     4261 ctgaaggctt gatcgagata ccgccggagt cgaaggttaa acctttaccc accagcacga
     4321 ttgggcgtgc atcttccgac gcgttgcctt tgtactcaat caccgacatc agcgattcgt
     4381 tttgcgaacc ctgaccgacc gccagatagg aatgcatccc cagctctttc atctgctgtt
     4441 cgccgataac gcgggtgatg acattcttgc tgtagctgtc agccagctgg cgcgcttgtg
     4501 aagcgaggta agcggcgtta cagatattcg gcggcatatt gccgagatct tttgctgctt
     4561 taatcccggc ggcaatcgcc agaccgtgca ggatcgcgcg ctcaccgctg gtcagttcac
     4621 ggcgggtcgg cacgttgaac accattttac gcagcggacg acgcggttcg ctcttgttcg
     4681 ttttcagctg atcgaaactg tagagcgtct cttttgccgt ctcgacagcc tgacgcactt
     4741 tccagtagtt gttacggcct ttaacgtgca gttcagtcag aaagcagacc gcttccattg
     4801 agccagtatc attcagcgta ttaatggttt tctgaataac ctgcttgtac tgacgctcat
     4861 ccagctcacg ttctttgccg caaccaataa ggagaattcg ctcggaaagt acattcggaa
     4921 catggtgcag caacaatgtc tgccccggtt ttccttccag ttcgccccga cgtagcaggg
     4981 cgctgatgta cccatcgctg attttatcga gctgttctgc aatcggagaa aggcgacgtg
     5041 gttcgaagac gcccacgacg atgcaggcac tccgctgttt ctccgggcta ccgcttttta
     5101 cactaaactc catgcactac gctcctgaat cttaaagaca acggcggtgg ctacagatag
     5161 aattgcaagc tttcgtaact catgtccgct gttgcgatga cttcgtgtta atcttaacgt
     5221 tattacggca ttggcacgtc agaacaaaat ctgagaggtg aatccgttga gtataattat
     5281 cttagcgacg atttcgacga ctcaagagaa taaatgacgt ttaagccatg aaacaagcta
     5341 aaatcctgca aaagacgagt ttttacgggc gtatttaaag tgataatcat aagatatctg
     5401 gtgcgggaga cgctcaaaag ccagctggcg atactcttca tcttgctttt gatcttcttc
     5461 tgtcaaaagt tagtgaggat cctcggcgca gcggttgacg gcgatattcc ggcgaatctg
     5521 gtgctctccc ttctcgggtt gggcgtgccg gaaatggcgc agcttatcct gccattaagc
     5581 ctgttcctcg ggctgctgat gacgctgggc aaactgtata ccgaaagtga aattacggta
     5641 atgcatgcct gcggcctgag caaagcggtt ctggtgaaag cggcaatgat ccttgcggta
     5701 ttcactgcaa tcgtcgcggc ggttaacgtg atgtgggcgg gaccgtggtc atcgcgtcat
     5761 caggatgaag tgttagcaga agcgaaagcg aaccctggca tggcggcgct ggcgcaaggg
     5821 caattccagc aagcgactaa tggcagctcg gtgctgttca tcgaaagcgt tgacggcagc
     5881 gatttcaaag atgtgttcct cgcgcaaatt cgaccaaaag gtaatgcacg tccttctgtg
     5941 gtggtggccg attccggaca tttaacccag ctgcgcgacg gctcccaggt cgtcactctc
     6001 aaccagggaa cgcgcttcga aggcactgca ctgttacgtg atttccgcat tacggacttc
     6061 caggattatc aggcgatcat tggtcaccag gcggtagcgc tcgacccgaa cgataccgac
     6121 cagatggaca tgcgtacatt gtggaacact gacaccgatc gtgcccgcgc agaactgaac
     6181 tggcgtatca cgttggtatt caccgtgttt atgatggcac ttatggtcgt accgctgagc
     6241 gtggttaacc cgcgtcaggg acgcgtacta tcgatgctgc cagccatgct gctgtatcta
     6301 cttttcttcc tgatccagac ctccctgaaa tcgaacggcg gtaaaggtaa gctggacccg
     6361 acgctgtgga tgtggaccgt taacctgatt tatctggctt tggcgattgt tctcaacctt
     6421 tgggacaccg tgccggtccg ccgcctgcgc gccagttttt cgcgtaaagg agcggtgtga
     6481 tgcaaccttt tggcgtactt gaccgctata tcggtaaaac tattttcacc accatcatga
     6541 tgacgctgtt catgctggtg tcgctgtcgg gcattatcaa atttgtcgat cagctgaaaa
     6601 aagccgggca ggggagttac gacgcgttag gcgcaggaat gtataccttg ctgagcgtgc
     6661 cgaaagatgt gcaaacattc ttcccgatgg cggctctgct tggggcgttg cttggtcttg
     6721 ggatgctggc gcagcgcagc gaactggtgg tgatgcaggc ttctggtttc acccgtatgc
     6781 aggttgcgct gtcggtgatg aaaaccgcca ttccgctggt cttgctgacg atggcgattg
     6841 gtgaatgggt cgcgccgcag ggcgagcaga tggcgcgtaa ctaccgtgcg caggcgatgt
     6901 acggcggctc gttgctctct acccagcaag gcttatgggc gaaagatggc aacaacttcg
     6961 tttacattga gcgggttaaa ggtgacgaag agttaggtgg catcagtatt tatgccttta
     7021 acgagaatcg tcgtctgcaa tccgtacgct atgccgctac tgcgaagttt gacccggaac
     7081 ataaagtctg gcgtctgtcg caggttgatg aatctgatct gaccaatccg aaacagatca
     7141 ccggttcgca gacggtgagc ggcacctgga aaaccaacct cacgccggac aaactgggcg
     7201 tggtggcgct ggacccggat gcactctcaa tcagcggttt gcacaactac gtgaagtatc
     7261 tgaagtcgag cggtcaggat gccggacgtt atcagctcaa tatgtggagc aaaatcttcc
     7321 agccgctatc cgtggcggtg atgatgctga tggcgctgtc gttcatcttt ggcccactgc
     7381 gtagcgtacc gatgggcgtg cgtgtggtca ccggtatcag cttcggtttt gtcttctacg
     7441 tactggacca gatcttcggc ccgctgacgt tggtttatgg catcccgccg atcatcggcg
     7501 cactgttgcc aagcgccagc ttcttcttaa tcagcctgtg gctgttaatg agaaaatcgt
     7561 aaccgaatga acgaataaag ggagcgaaag ctccctttat tattgttagc aaagtgtgct
     7621 tcgttcatgc ctgaaaaatc atgaaaatcc aatatattgc agtggtgtag gcatgataag
     7681 acgcgccagc gtcgcatcag gcaaagacaa atctcgcctg acttaccttc ttcttccccc
     7741 cagcaaactc cccaacatcc cacgtacaat ctgattcgtc acctggcggg cggcgctttt
     7801 cgccattgtt tgcaccacac catctttctt cccgccgcgt ggtccggtag tgccaaacaa
     7861 aatatccttc aatccaccaa gaataccgtc atccaccgcc accgctttcc ctttcgcggg
     7921 aggattattt tgctgcttgg tactggcctg aaagcctttt tgcaacatct cataggcgga
     7981 ttcccggtcc acgtcatcct cgtacttgcc atacaccggg gagtggttaa tcaagccatt
     8041 gcgctcatct tccgtcaccg gtcccatccg cgaacaaggc gcaatcacca tcgcccgttc
     8101 caccacagaa ggacttcctt ttgcatcgag aaacgagatt aacgcctcgc cggtccccag
     8161 ttcctggatt gccttttcgg tatcaaacgc cggattggcc cgcatggttt gcgccgcagc
     8221 ttttaccgct ttctgatctt tgggcgtaaa agcccgcaaa gcgtgttgaa cgcgattacc
     8281 gagctgcccg agcacattat ccggaatatc agacgggttt tgcgaaacga accagacgcc
     8341 cacgcctttt gagcgaataa gccttattac ctgctcaatc ttatccagca gtacctgcgg
     8401 tgcatcgtta aacagcagat gtgcctcgtc gaagaaaaac accagttttg gtttctccag
     8461 atcgcccgct tccggcaatt gttcatacaa ctctgaaagc atccacagca ggctggcggc
     8521 gtacagtttc ggcatctgat aaagcttctc ggcgctgagg atattgataa cgcctttacc
     8581 gttggtatcg gtgcgcatcc agtctttgat atccagcatc ggctcgccaa agaaatgcgc
     8641 cgcaccttgc ttttccagcg acagtaaacc gcgctggatg gcaccaaccg atgcgctact
     8701 gatattaccg tactgattct ggaaagattt ggcgttatcg ccgatgtact gggtaatcgc
     8761 ccgcagatct ttaaagtcga gcagcaacaa tccctgatcg tcagcaatac ggaagatgat
     8821 attcagcacg ccagattgca catcgttgag attcaacagc cgcgccagca acagcggccc
     8881 caggtctgaa accgtcgccc gcaccggatg gcctttctcg ccaaagatat cccacaccac
     8941 caccggatta gcatgcggtt gccagtcatt gacgccgata tttttaagcc ttgtgagcag
     9001 tttttccgac gccgttcctg cctgcgcgat accggtcaga tcgcctttca catcagccat
     9061 aaacaccggc acgccgattt ccgacaatga ctctgccagt ttttgcagcg taacggtttt
     9121 acccgtcccc gttgcgccag taatcagccc gtgacggtta gccattcccg gcagtaaaaa
     9181 aagttctgtg tccggcgtgc gggcaattaa taggggttcg ctcatgagat ttcctccagt
     9241 ttcctgcctg gagtataggc aacggctgcg cactcttcac cgctaaattc agcagattgc
     9301 gagacgcctt ccaaagagat ttaagtgccg ggcgacaggc acccggcaaa gggatcagaa
     9361 gtcggctttc agaaccacgc ggtaacgcgc cttaccgtcg cgcacatgct tgatggcatc
     9421 gttaattttc gacatcggga acagttcggt ggtcggcgca accttgctac gggcggcaaa
     9481 gcgcatcagc tttcgcagct cataaggcgt gccggtagca gaaccagaga tgctgcggtc
     9541 gcccgcaatt aacgtaaagg ccggaacaga cagcggcgtg agaaccgcac cgaccgtatg
     9601 gaaattaccg ccataggtca gcgcctcaaa ataaggctgc cagtcgaggc tgacgttcac
     9661 agtattgata atgagatcaa actgccccga cagggctttc agtgcctgcg gatcgcggct
     9721 attcaccact ttatcggcac ccatcgccag tacttcctgc tctttcgccg gattagaact
     9781 aaaggccgtc acctcgcatc ccattgcgtg cagaagtttt atagcgatat gccccagccc
     9841 gccaatacca attaccccaa cgcggctggt agcagtgata tggtgcatca acagtggttt
     9901 aaagaccgtg ataccgccgc acaacagcgg cccggcggac tcgatatcaa tattttctgg
     9961 cagtggaatc acccattgcc agtccgcacg caacttctcg gcaaagccac cgcgattcat
    10021 aatcgtcggc accgcacctt gctcgcagtt gatctgatta ccgctaatac aggcgtcgca
    10081 gtgaccacag ctacgcgccg tccagccaat cccgacacgc tgaccgacct gcaaaccttt
    10141 atcctgcgcg gcgctcccga gtgccaccac gcgcccaatc acctcatgcc cggcaaccag
    10201 cggatattgt gaaaatcccc attcattgtc gatcatcgat agatcggaat ggcagatccc
    10261 gcaataatcc acctgcactt caacatcttg tggcctcagc tcaccgggat cgtactcata
    10321 aacttccagt tcgccgcccg cttcttttgc ggcatagctt tttatcatcg acattttttg
    10381 gtccttctct ggtgttgttt gggaagtgta gagcatggca gggcggggtg cctggagtgt
    10441 gacaaaggtt acacatcgct gtgtgcaatg ctgaaaattt cagcacttag cgaggtgcga
    10501 gcaagctggc gcttgcatgg tggcgtgcga caggtataat ccacaacgtt ttccgcatac
    10561 ctcttcagtg ccgaagtggc gaaatcggta gacgcagttg attcaaaatc aaccgtagaa
    10621 atacgtgccg gttcgagtcc ggccttcggc accattctta cttccaagac catccgagaa
    10681 agtccaaata tccctttaaa atcaatgctt gcagcgattt tttcgtcctg cgtcttccga
    10741 ggttgtccgt tgaaatccgg atatcattgg gggcataatt gggggcatct taacttcgat
    10801 tagaaatgtg cccccaaatg aagctcaacg ccagacaggt cgagaccgca aagccaaaag
    10861 acaaaaccta caaaatggcc gatggtggtg gtttgtatct tgaggtttcg gccaaaggtt
    10921 ccaaatactg gcgcatgaaa tacagacgtc cctctgacaa aaaagaggat cgtctcgcgt
    10981 ttggtgtttg gcctactgtg acgcttgctc aggcaagagc aaagcgcgat caagctaaaa
    11041 agctgttagc gcagggcatt gatccaaaag ccgaacagaa agaagctcag gccgagaatt
    11101 cgggggcata ttctttcgaa actatcgctc gtgaatggca tgctagtaat aagcgctgga
    11161 gtgaagatca ccgatcacgc gttctacgtt atcttgagct ttatatcttt cctcatattg
    11221 gttcatctga catccgtcag ctcaaaacta gccacctatt agccccgatt aaaaagttga
    11281 tgccagtggt aagcatgacg ttgcacagcg ccttcagcag cgtgtcacgg ctattatgcg
    11341 ttatgcagta cagaacgatt acatcgactc aaatccagcc agtgatatgg ccggtgcgct
    11401 atcgacaacc aaagcgcgac attaccccgc tttgccctcc agccggttcc ctgagtttct
    11461 tgctcgtctt gctgcatatc gtggccgtgt aatgacgcgg atcgcggttg agctttcctt
    11521 actaactttt gtgcgttcca gtgagttacg tttcgcgcgt tgggatgagt tcgacttcga
    11581 taaatctctc tggcgagtac ctgcaaaacg agaagaaatt aaaggtgtgc gttactcgta
    11641 ccgtggcatg aagatgaaag aggagcatat cgttcagctt agtcggcagg cgatgatttt
    11701 gctaaaccag ttaaagcaga ttagtggaga taaagagctg ctttttccgg gtgatcatga
    11761 cgcaactaag gttatgagcg aaaacacggt aaatagcgca ttgcgtgcga tgggctatga
    11821 cacgaaaacc gaagtttgcg ggcacgggtt tagaacgatg gcccgtggtg cgttgggcga
    11881 gtccggatta tggagtgaag atgctataga acgacagttg agccattcag agcgtaataa
    11941 tgtacgtgct gcttatattc acacctctga acatttggat gaacgtcgat tgatggttca
    12001 atggtgggct gattatctcc aagcaaataa gggcaaaaca ataacacctt atgattttgc
    12061 taaaataaac aaaagtcgat agtgaaaata aacgcctata atatataggc gtttatttct
    12121 gtattaaaca ctttgatcat aatcttccag cagcaacttt ttttcgtact tcatctttcc
    12181 aattgccact atggattatt ctatcgaaat tacttcctgc tcttcctgat aataagtcaa
    12241 ttcgagatag cttgctgata tcatcccagt tatcatagag gatatatata gcattaccat
    12301 attctatgtt ttcaaataca accaaatcgt cctccagcat agctccaata taattactga
    12361 acgtgctgtt tcctgtgacg atttttttag gttcaagact catgaagaag tcatatctat
    12421 tttcagcgac atttttcttg tcggaagtga tatttttacc tctaaaaatt cttgctaaag
    12481 tttcttcttt actacctggt ggaaatattt cccatgagac tataagtgta tcagcataag
    12541 tcgatatggg tttatcagca tcctcaattc cacatttacc aagattttct tgaagaagat
    12601 ttaaatcaaa taataattca tcatcaaact tatttgattg tttgtctaaa attctagtta
    12661 ctctgaagtt aaatatgaaa tggctgtccg agggctgttt gtgattaatt tcaatggcta
    12721 tgagtgttgg tggtataatg tctctgggat atttctcgta aggtaatcta acagtatgtg
    12781 taccattaga gctatcaccc cagttaggtg actcaatttc agtgtaatga gtttcttttg
    12841 ggaggtcttt tctgacaatt tcttcaccaa aaacattttt aagtgaatat ttaccctgca
    12901 atggatctgg tatgaccgac gtctcataag cattaacacc attatcaaaa tatattccaa
    12961 ggtgctctaa tgctccactt tttaattggt tttccgtaaa tgcaatagaa gtgccagcaa
    13021 caactgtatt accaagcctt cttaacttgc tatgaatatt tttaggtata acacgtatat
    13081 tctttttcat ttttccctca tacttgatca tcaatgtctc tttgctctcg cttgttatgt
    13141 aaatcattga taaacataac atttgcaagt attacgctaa ttactgcctc aatctgcatg
    13201 tacgaaatag caataattaa tgctaaagat gtgtttttta ttacatcttg gctctctaaa
    13261 taaagatgac ttttgaaaaa cactagttta actaagtata caacaagtaa agtaatcata
    13321 acgaaagatg attttagaac acttccaact attgcttcta atcttcttgt atcttccagt
    13381 tgttcggtga aatcaacggt ttctatagtt ttggggttca caatacgctg caaagcattt
    13441 gggtaaagaa acgctaacca aattcccata atagtaaaaa ccattccaga aactgatacg
    13501 agaagagtgc aatagttggc aaaatcgtta tacacatagt taatatcgga tgacgaaata
    13561 atcatcagcg atataatgaa tatcgccatg tatatccatg tgtatttggc tatgcctgct
    13621 atagctttca atattcaatc ctctccagat gctttcttta ttcttggttg agattgtaat
    13681 gcttttaatt cttttaatat aggggaaata aattcttttc ttttgtgtga tatttgttcg
    13741 taaatatatg cggcggaata tgttgaatag tcgtcagaag ggacattgag aatatcacgc
    13801 attctatatt tatccaccca agttataccc ttgtctgtag caaatccaac attgtcccaa
    13861 agacgttttt ctctatcctc agccgaatac ttttcaatga tttcttgaac ttcattcaag
    13921 gatggttttg cttctgcttt tatttcaatt tgtcttgttt tggcattttt ctttccggac
    13981 acaaatggaa cgagagtgct aaaggtcttt aaccaatcag ctcgtgcatc atttggattt
    14041 ataataatag tttccctgcg aactatgtgt gttatttttg gtattagttt acctaactca
    14101 gtatgagtgg tttctaagct tctgagcttc atatcaaacc tgtacatata tttatataag
    14161 tcttcgtcat cggtattaga tagccttata taacctttct cggttttatt tttaaccctt
    14221 ttgctatgtt taactctatt agtaatagat gagtgtacat agtcttgaaa tagttcagaa
    14281 tcacaaacag aatggtcgaa tttaatggat actatagttt caagttcagg tataacccaa
    14341 taaaagcatg gccgccccca gataactttt ttccctctgt agctattagt atatttaacc
    14401 accgatgagc ttccgatttc accgtcttca ctagctccta ataaagagcc cgtactatca
    14461 gtatcagatt tccataacat gactaagaaa tcaccagtat tttcatcctt ataaatatcc
    14521 ttgcaataac actttgaact gtttggtttt tcttttggat cccatggtat cgtgagtgca
    14581 agagatttag ttcccaccca atcctgtatg aggtcaaatg tttcgctaag ttctaaacca
    14641 taggtattgt catcatttac cttgtataaa ccgcacttat taaccttgaa gaaattgaga
    14701 tgccctacat tcatgctctt tccttagctt cttatcttgg gatatgtatc atgatcaaaa
    14761 attaagcata accccattga cttcgtgcgg gcaatgtttt caccctagaa tttgatcggt
    14821 ttaacgttaa atattcataa gttacatgag catttttcac ttcttcccaa ttacagacgt
    14881 catgagctga atattaataa aataattatc ctcacggcat ggcgcgcaat gctctccccg
    14941 ccacgcccgc ccgctttgca gggcggtttt aatgcagttg cacagtcacg ctcaggctgc
    15001 gccgggaatg gcgcggtctg cagaaaatga ggcaggaaaa cgcatgcaaa gccatgcacc
    15061 ttatcgatgc atggcttttt tcagtaaaaa cgggcggatt ttcggggaat tttacacaga
    15121 ctgacgtgat gccagttgcg cacttttacg cgaaaaaatc atgttctgcg caggggtgaa
    15181 tttttcacgg ctgtcatcca ccgaagtcgc gtcaggcctg aatccgatgg tcgttaaaat
    15241 gtcactatcc tgtgcggaat aattaatttt ttcccccttc gcaagccagg acagaagggc
    15301 ttcacgcagg gcatctgtgg cacgctgtat ggcacagttt cgggcaatgg ccgtcagctc
    15361 actgtagccc atcagctccg gtgccagtgc cgccgccagt gctgtgccgt gctgctgcat
    15421 aaaatcattc agccggtcgc ggatgctgat gtgctgaacg gcttcatgcg aacgaatata
    15481 acgaccggca gcctgattca cctgccattt tctgacttcg ataatattgc gtaattcgtc
    15541 caggcgactg acgtttctgc cttccccgga cagaagccgc agatattcct gttcggccgc
    15601 agccagctca tttttgcgtt gcagccatgc tgctttgtta ttctgacagg tgtcaaagac
    15661 ctgctgtaag gctgtgcttt ccatcgttat ctctttctca tcatgctgaa gaataaaaat
    15721 acggtgtgcg gcgacggccg gtgttaaccg gcagccctca ttccagacgc agcgaatatg
    15781 attgtgtttt taagcgtact ggcggcagtt cctgtttttc atgcaggctt tctgccagtt
    15841 cgtccggcgt gaccgggcgg acaataaaac ggttgatggt ctgaagcgtt ttaaacacca
    15901 gaccacagcc cggatccgtg cacacataaa aacgctcggt gacttcctga gacagacgcc
    15961 gcgatgttct tgacagtgca aggcctttac atctccgaca acaatatccg gtaacaagca
    16021 ttcttttcgg gcgtttcata ctgccggagg ctgacgtcag tgaatcgcgg tatctctgtt
    16081 tgcctgaaat gtattccatt cctgaatctt tacagtcaga gaaaaagctt tcactcgctt
    16141 caaatgtcgc agagcaataa atattccggc actgtgcaat cattatcttg gtgccatcgt
    16201 ccatgaaatg tgcgcgacgg gtgtgagcaa catgtccaca cgacgggcag taaatcatga
    16261 cagcagtcct ctggccttaa gctctgctcc ctgctggtct attttgtcct gccacacctt
    16321 gcgctgtgcc ggtgtgcctg ccacctcata atccatgtgc gggagtgttg ccgctgacag
    16381 tccggtcagc cggagaaccg gctcgccggt gaggctgatt tgcatctgtt taattttctg
    16441 ttccagcgat gatttcacct gctgcatgac agccttttcc ggtgcgacgt agccctgatg
    16501 gccggtggtg ttggcgagcg gattttcctg taccaggata ctcagatgca ttgcccggac
    16561 aagcgcctca caggtttcat tcagggcgtg ttccagctca tgctctgcat acagactcag
    16621 caggtgatga tgtgccttcc ggtaggcggt ggccgtgctg tcacacgccc ctttcaggcg
    16681 ttcacgttca aaattcagca ccacggccag attgtcatat tcctgtacca gctcccggcg
    16741 tgccacgcgc tcaatgtggc gctgtttcag ctcgtcgctc aggacaccac cggctgcacg
    16801 aaaggccgtg cgccagtcgt cagcgtcatt tccgtcggcc tgcgccagcg catttttttc
    16861 ctgctctgcc cgttcaatgg ccgtgacggt ctcatccatc aggcgggcgt tctcaaggtg
    16921 ggcggctctg gccttttcca gttgtgccag tgcgggttgc agatattcag ggatggtgtt
    16981 gtcagacatt ttccggctcc tcgtcacttc aggttaagaa aattgtgacg tacaccggac
    17041 aacaacacga cgcattgcag atgtgccagc cctgacacag gagactcatc ctcagaccgg
    17101 caagccagga aaaggtcgca ggaaaaaccg gcttactgtt tgttttttta tattttactg
    17161 ttcacctctg ttcaccataa gaaaaaagat aagtaataca gtaagttaaa gggtgaacaa
    17221 tcgcagtaat gactgttcac cgtctgttca ccactgttca cccgctcata gactttttgt
    17281 gctgtttatt actttttatt tttattaatc cactgaaata aataagaaaa aacaatttgt
    17341 atttcactat aattttttcc agctccttcc agagcgtttt aaagccatct ggcgctgatg
    17401 tataaaaaat acacagccat tgtaagactg ccggaacaaa tcccccctgt tgcatctgcc
    17461 aaaaatattc acaaaataaa gcgctacccg aagccggacg gacttatccg gtgctgtatg
    17521 gacattaacg aggtagcccg atgcaagctg ttttttcttc cccgtctccc gcccctgtga
    17581 cgccactgat gccgctgccg gacatcacgc aggagcgttt tttacgtctg ccggaagtga
    17641 tgcacctgtg cggcctgtca cgctcgacca tctatgaact catccgtaag ggggaatttc
    17701 cgccgcaggt gagtcttggc ggtaaaaatg tggcctggct gcactctgaa gtcaccgcat
    17761 ggatggccgg acgcattgcc ggacgcaaac gggggtacga cgcatgatga tgcccgctct
    17821 gcaaaaactc cctttttctg gcttgccttt ttccggcatt tgcggatata gtttttccgc
    17881 tgccgcaaaa tcggcagccg ggcgtgagaa cccgagcaat tcacaggcga catatgacgc
    17941 gccacgcgtc tttttttgtg tcgcaatcaa tgccacagag cgccagatta tggtgtggtg
    18001 tgtggtcagt cgtgcagatg gcattccgtg catcctgccc gcgtccgctc attatgccgc
    18061 agagtcaatg gtagctcagg cggggcagcc tccgggctgg ccggtatcct gtgaagccgg
    18121 tattctcacc cccgtctggg ctatcgccat cgagcgtgag aactccggcg atagcgttat
    18181 ttgctattca caggaggctg ccatcatggc tacaaccctt accccctcac accctgaatt
    18241 tgtctttgtg tttgcggctg tccgtcgcgc agaccgtcat ccccgtatct gtatgcttcg
    18301 caccgtggcc ggtgatgaac gcagtgcccg ccgttccctt gtccgtgact atgtgctctc
    18361 ccttgctgcc cgtctgccgg tggtggaggt gtcccgtgcg taataaaaaa gcccctcaga
    18421 ccgtctcagc acgtcatgac gcccgtgaac acctcagcat tgaggcttac cataagctca
    18481 accgcgccag cgccgtatcc cagtttgttg ggggtgattt gattcaccgt gaactctccg
    18541 gcctgcatca gctctacatt ccgcatattt tcagctacct gaatgaagat attgattttg
    18601 tgctgaatga gctgaaagcc aaaggcctgt gccgcgattt tctcgcccag cagaaagacc
    18661 ggggagacag gacgcatgtt tgattttccc cagcccggtg agatttaccg ttctgccggt
    18721 tttcccgatg tggccgtggt cggcattctg gaagacggta ttccgtggga aatgccgtac
    18781 cgctgcccgg acattgtctg gaacccgtac cgccgtaaat tcagtatcct tgtgcgtatc
    18841 ctcgccgacg ggcgcaccac agacatcccg ctggggcgtt ttctgcggga atttacctgt
    18901 gaccgtcctg acctgtttaa acgcagcccc gtaaaccggc atgcggtact gaaagaaatg
    18961 gccggagacc cggaattaca gaaatggcgg gagaaatatc tggatattta cccgcaggac
    19021 actgttccgg tcagccgggc agcaccggta gcgcgggaat ggcgggaaat tccccgcacg
    19081 gagcctgacc cggaaatcac cccggataac agttaccgca attatctgta attaaaaaac
    19141 gacacccgaa aaattaaatg tgcgtattcg cgcagggata cgcacgtctt caggagacgc
    19201 agatatgcct tatcagttaa tgcaaccggc acggaatgca gtcatctgtc acagggagga
    19261 aagcaaatga aaacaccctt accgcccgtc ttacgcgcag ccctttaccg tcgtgctgtc
    19321 gcctgtgcct ggctgaccgt gtgcgaacgt cagcaccgct acccgcatct cacccttgag
    19381 tcactggagg cggccatcgc cgctgagctg gaaggctttt atctgcgcca gcacggtgag
    19441 gaaaaagggc gtcagatagc ctgtgccctg ctggaagatt taatggaatc cggccccctg
    19501 aaggccgcac cgtcgctgtc ctttctcggg ctggttgtga tggatgaact ctgtgcccgt
    19561 cacataaaag cgccggtact gcactgaagg agaacaacac catgaaaatg aacgtaaccg
    19621 ccaccgtcag ccatgcgctc ggccactggc cgcgtattct cccggcgctg gggattcagg
    19681 tgctgaagaa ccatcatcag ccctgtccgg tctgtggcgg gagtgaccgc ttccgttttg
    19741 atgacaggga ggggcgcggc acctggtact gcaatcagtg tggtgccggt gacggcctga
    19801 aactggttga aaaggtgttt ggtgtctccc cgtccgacgt ggccgcaaag gtggctgccg
    19861 tgaccggcag tctgcccccg gctgacccgg cagtgacggc cgccgccgtt gctgaaacag
    19921 acgctgcccg gaagaacgcc gccgcactgg cacaaaccct gatggcaaaa acccgtcccg
    19981 gaaccggtaa cgcctacctg acccgcaagg gctttcccgg ccgggaatgc cggatgctga
    20041 ccggcacaca cagagccggt ggcgtgagct ggcgcgccgg tgaccttgtg gtgccactgt
    20101 atgacgacag cggcgaactg gttaaccttc aattaatcag tgctgacggc cgtaagcgca
    20161 ccctgaaagg cggacaggtc aggggcacct gtcacaccct tgaaggacag aatcaggccg
    20221 gaaaacgtct gtggatagcg gagggatacg cgaccgcact taccgtacat cacctgaccg
    20281 gtgaaacggt gatggtggcg ctttcttccg tgaacctcct ttctctggcc agccttgccc
    20341 ggcagaagca tccggcctgt cagattgtcc ttgccgcaga ccgtgacctc agcggtgacg
    20401 gccagaaaaa agccgccgca gccgcagatg cgtgtgaagg tgttgttgcc ctgccgccgg
    20461 tcttcggtga ctggaatgat gccttcacgc agtacggcgg ggaggtcacc cgtaaggcca
    20521 tttacgatgc catccggcca ccggctgaaa gcccgttcga caccatgagc gaagcagagt
    20581 tttccgccat gagtaccagc gaaaaggcca tgcgtatcta tgagcattac ggcgaggcgc
    20641 tcgcggtcga tgccaacggc cagcttctgt cccgctatga aaatggtgtc tggaaggtgc
    20701 tgccgccact ggactttgcc cgggatgtgg ccgggctgtt tcagcgtctg cgcgcgccgt
    20761 tctcctccgg gaaggtggcc tccgtggtgg acaccctgaa gctgattatt ccgcagcagg
    20821 aagccccctc ccgccgcctg attggctttc gtaacggtgt gctcgacaca cagaacggca
    20881 cgttccaccc gcacagtccg tcacactgga tgcgtaccct gtgcgatgtg gatttcaccc
    20941 caccggtgga aggggaaacg ctggaaaccc acgcccccgc gttctggcgc tggcttgacc
    21001 gtgccgccgg tggccgtgcg gaaaaacgcg acgtgattct ggccgcactg tttatggtgc
    21061 tggcaaaccg ctacgactgg cagctctttc tggaggtgac cggtcccggc ggcagcggca
    21121 aaagtatcat ggccgaaatc gccaccctgc tggccgggga ggataacgcc acgtcggcca
    21181 ccattgagac gctggaatcc ccgcgtgaac gtgccgcgtt aactggcttc tcactgatac
    21241 gcctgccgga ccaggaaaaa tggagcggcg acggtgccgg actcaaggcc atcaccggcg
    21301 gcgatgcggt gtccgtggac ccgaaatacc gggatgcgta ctccacgcat atcccggcgg
    21361 tgattctggc cgtgaacaat aacccgatgc gcttcaccga ccgcagcggc ggcgtgtcac
    21421 gccggcgggt gattattcac ttcccggaac agatagcccc gcaggagcgc gacccgcagc
    21481 ttaaggacaa aatcacccgc gagctggcgg tcatcgtgcg tcacctgatg cagaagttca
    21541 gcgacccgat gctcgcccgg tcactgcttc agtcccagca gaactcagac gaggcgctga
    21601 acatcaaacg ggatgccgac ccgacgtttg attttatcgg ctatctggaa accctgccgc
    21661 agaccagcgg catgtatatg gggaacgcca gtatcatccc gcgtaattac cgtaaatacc
    21721 tctatcacgc ctatctggcc tacatggagg caaacggcta ccggaatgta ctcagtctga
    21781 aaatgttcgg gctggggctg ccggtgatgc tgaaggaata cggactgaat tacgagaagc
    21841 gccataccaa acaggggata cagaccaacc tgacactgaa agaggaaagc tacggcgact
    21901 ggctgccaaa atgtgacgac cctgcaacaa cctgacccac ctgaccggca tctgccggtc
    21961 tttttttatc ccgacctccc ccgaaggtga acaatccact gttcaccctt caccgtatat
    22021 tcacccgtta tcacactgaa attaaaagag aaaaatgaaa ggtgaacagt gtgaacaatc
    22081 aaatcaaaaa aaactttttt ctccctgtgc gattttcagt acgggggatt aatcaccggt
    22141 atgagtcaca ccggcagaat gccggaggtg aagaatcgaa tgttcaccct tcacccatta
    22201 ttcaccacct atcactctga aataaaagga gaaaattaaa tggtgaacag tgtgaacagt
    22261 tctttcgaaa aaaaattttt tatgtggata aaagatgcac tggtttggat ccgggctacg
    22321 gatccaaact tactgattaa aaattattta actttccgat ttattgatgg gctgaaaata
    22381 gattggtcag aaaaacagtg ggggcacaaa agggggcata tttcattgtt gtgttttatt
    22441 atttgttttt tatcattaac ttattttgat atttgagtcc ggccttcgca ccaaaagtat
    22501 gtaaatagac ctcaactgag gtcttttttt atgcctgaaa tccgcgctac acaaggcttt
    22561 tccagctttt cccctcaact gaagtcaacc tgactcaacc cacatcaaca ggcttgtgag
    22621 tatactaatg agtatatttg ccgattcgat attgcttgta tactcacgag ataccaatgg
    22681 aaggaagacc attatggccc taacggatac taaagttcgc tctgcgaaac ctgaggagaa
    22741 agagtattca cttgttgacg gcgacggcat gtccttactt gtaaaacctg gcggttcaaa
    22801 gtattggcga tttcgtttcc gttttggcgg taaacaacat ttgatggcgt tcggcgttta
    22861 cccggatgtt tcgctggcgg atgcgcggaa gaaaagggaa gaggccagaa agctggtagc
    22921 tgctggtatc gatcctcgtg agcataagcg tgctgtgaaa gaagagcagg cgaaagagat
    22981 tattactttc gagaaggttg ccagagagtg gctcgtaacc aaccaaaaat ggtcggaaga
    23041 gcatgctaat cgtgtaaaaa agagcctgga ggacaatatc ttcccggcaa ttggttccca
    23101 caatattgct gaactgggca cccgcgatct gttaattcct attaaagccg tggagaaatc
    23161 cggacgtctt gaagtggctt cccgccttca acagcgcacc acggccatca tgcgttatgc
    23221 agtgcaaagc gggttaattg attacaatcc ggcgcaagag atggcggggg cggtagcttc
    23281 cagtaatcga cagcatcgtc cggcgctgga gttaaagcgt atcccggaat tactccagaa
    23341 aatcgatgaa tacaccggca ggccactaac ccgctgggca accgaactta ccctgcttat
    23401 ttttattcga tccagtgagc tgcgttttgc tcgctggtca gaaatcgatt ttgaaacgtc
    23461 aatgtggacc atcccgcctg agcgagaacc tattcccggt gtgaaacatt ctcagcgagg
    23521 agcaaagatg cgtacacctc atctggtgcc gctttcaaag caggcgctag cgatcctgaa
    23581 gcagataaaa cagttctgtg gcgaacatga gctgattttt attggcgatc acgatccgag
    23641 taagcccatg agtgaaaaca cggtgaacag tgcattgcgg gtgatgggct atgacaccaa
    23701 ggttgaggtt tgcggccacg gcttccgcac tatggcctgt agctcattga ttgagtcagg
    23761 gttatggtcg aaggatgcgg tggaaaggca gatgagccat atggaacgca attcggtgag
    23821 ggcggcgtat atccataaag cggaacatct ggatgagcgc aagctgatgt tgcagtggtg
    23881 ggctgatttt ctggatgcga accgggagaa ggggattacg ccgtttgact atgcgaagat
    23941 caatcggggt aatggcgagt gagttatcta aaagcgcaca ttcgcctcta aattgaaagc
    24001 tgctgactta ttcaaacgag gaattggtca gcagtgccaa atagccgagt tttcactaag
    24061 gtaagccttt ttattgaacc taatcggcag taatgctcca cctaagccta aatttaaagt
    24121 catgattact tataaaaggg gagtggaccc ttgattgagc cctacatcgt aacaaaggta
    24181 cagcaattcg gctacatcag gagtgatagt caattagtta atctaataat taattgaatc
    24241 taaataatta aatttcttac gtaaaattga ccaatttact ggtggtgtaa gccgcaatat
    24301 cttaatgtaa tcagtataaa ggggcttgat ttcttttaaa gatagtatcc gtatgaattt
    24361 taagaaaaaa ctcgaagagc attttaagca gttcgaagca tccccagtgt tatttgtcgg
    24421 ctccggtgtg tctcgtcgtt atcttggtgt gccatgttgg caagaccttt tgaagcattt
    24481 tgctgaggca attggggaaa atcatataaa attaaaaaca aaatctaatg gtgacttacc
    24541 cgaatatgca caattattgg tgtctgcata tgctgagaaa tggtgggata ctgaagaagg
    24601 acaattagca ttaagtgaaa aagaacagga gaaaactttc attaacgaac agtctccttt
    24661 gaagttgtca atatctaaat atattgagaa tgcacataaa aatattatag ataatgatga
    24721 gttaaaacat ga
//
LOCUS       DADLFA010000046        24719 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000046 DADLFA010000000
VERSION     DADLFA010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24719
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.046"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(47..337)
                     /locus_tag="JQH65_001566"
     CDS             complement(47..337)
                     /locus_tag="JQH65_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY6139026.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGKNL"
     gene            complement(596..1477)
                     /gene="yddG"
                     /locus_tag="JQH65_001567"
     CDS             complement(596..1477)
                     /gene="yddG"
                     /locus_tag="JQH65_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HAY6139027.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEAGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTQQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            1709..4756
                     /gene="fdnG"
                     /locus_tag="JQH65_001568"
     CDS             1709..4756
                     /gene="fdnG"
                     /locus_tag="JQH65_001568"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2294..2296,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HAY6139028.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIAVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLAGQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGERVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            4769..5653
                     /gene="fdxH"
                     /locus_tag="JQH65_001569"
     CDS             4769..5653
                     /gene="fdxH"
                     /locus_tag="JQH65_001569"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HAY6139029.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            5646..6299
                     /gene="fdnI"
                     /locus_tag="JQH65_001570"
     CDS             5646..6299
                     /gene="fdnI"
                     /locus_tag="JQH65_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HAY6139030.1"
                     /translation="MSKSKMIVRTKFIDRVCHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(6350..6634)
                     /locus_tag="JQH65_001571"
     CDS             complement(6350..6634)
                     /locus_tag="JQH65_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HAY6139031.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(6780..7790)
                     /gene="adhP"
                     /locus_tag="JQH65_001572"
                     /pseudo
     CDS             complement(6780..7790)
                     /gene="adhP"
                     /locus_tag="JQH65_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
     gene            complement(7924..9621)
                     /locus_tag="JQH65_001573"
     CDS             complement(7924..9621)
                     /locus_tag="JQH65_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAY6139032.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     IDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            complement(9778..9915)
                     /gene="sra"
                     /locus_tag="JQH65_001574"
     CDS             complement(9778..9915)
                     /gene="sra"
                     /locus_tag="JQH65_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HAY6139033.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            complement(10017..10232)
                     /gene="bdm"
                     /locus_tag="JQH65_001575"
     CDS             complement(10017..10232)
                     /gene="bdm"
                     /locus_tag="JQH65_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HAY6139034.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            10578..11009
                     /gene="osmC"
                     /locus_tag="JQH65_001576"
     CDS             10578..11009
                     /gene="osmC"
                     /locus_tag="JQH65_001576"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HAY6139035.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSFMLGEAGFTPTSIDTIADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            complement(11065..11991)
                     /locus_tag="JQH65_001577"
     CDS             complement(11065..11991)
                     /locus_tag="JQH65_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAY6139036.1"
                     /translation="MSDTLLTLRGVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALVEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATSGCEVRQSLAIREDGRELRCWRAL"
     gene            complement(11984..12970)
                     /locus_tag="JQH65_001578"
     CDS             complement(11984..12970)
                     /locus_tag="JQH65_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY6139037.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIVLLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVRADVIHHPRHPYTIGLLQCAPEHGVPRQLLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            complement(12967..13863)
                     /locus_tag="JQH65_001579"
     CDS             complement(12967..13863)
                     /locus_tag="JQH65_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HAY6139038.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLIWMLKGSPLTVTGAVIIVLML
                     LMMFFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            complement(13860..14882)
                     /locus_tag="JQH65_001580"
     CDS             complement(13860..14882)
                     /locus_tag="JQH65_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY6139039.1"
                     /translation="MTFWSILRQRCWGLVLVMAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            complement(14884..17019)
                     /gene="ddpX"
                     /locus_tag="JQH65_001581"
     CDS             complement(14884..17019)
                     /gene="ddpX"
                     /locus_tag="JQH65_001581"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: protein motif:HMM:NF013586.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HAY6139040.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLNSPVLYPLAHSTSVNQETVMKRSIPFRPTLLALVLATTFPVA
                     HAAVPKDMLVIGKAADPQTLAPAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGD
                     LASSWKASDDQKEWTFTLKDNAKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLK
                     IDAPDEHTVKFTLSQPFAPFLYTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGP
                     FMLKSWQKGQQLVLVPNPHYPGNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPV
                     DQLNALKQENKVNVAEYPSLRVTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILS
                     GNGKQMRGPIPEGMWGYDATAMQYNHDETKAKAEWDKVTSKPTSLTFLYSDKDPNWEP
                     IALATQSSLNKLGINVKLEKLANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFE
                     SDKKGLPGNRSFYENSEVDKLLRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKN
                     YQLAMNKEVKGFVFNPMLEQVFNINTMSK"
     gene            complement(17277..19676)
                     /gene="dosP"
                     /locus_tag="JQH65_001582"
                     /gene_synonym="pdeO"
     CDS             complement(17277..19676)
                     /gene="dosP"
                     /locus_tag="JQH65_001582"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HAY6139041.1"
                     /translation="MKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNQHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFRGKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNHDYL
                     LSTAHNAIDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            complement(19701..21082)
                     /gene="dosC"
                     /locus_tag="JQH65_001583"
                     /pseudo
     CDS             complement(19701..21082)
                     /gene="dosC"
                     /locus_tag="JQH65_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
     gene            complement(21454..22773)
                     /locus_tag="JQH65_001584"
     CDS             complement(21454..22773)
                     /locus_tag="JQH65_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HAY6139042.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIT
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            complement(22904..24439)
                     /gene="gadC"
                     /locus_tag="JQH65_001585"
     CDS             complement(22904..24439)
                     /gene="gadC"
                     /locus_tag="JQH65_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HAY6139043.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
BASE COUNT         6171 a         6225 c         6221 g         6102 t
ORIGIN      
        1 tgtaatataa ggtcagtgat catattgtga tgggcactta actcaatcag agattcttgc
       61 cgcgcgagcc gttggcttca tcgttatagc ctttaaaatc atattcccag tgaccaaaac
      121 caatcatttg atcattgatt tgcgtttctc ctttaaagcc gagacgggca taagttttat
      181 caccatcgtc acgcttatca tcagtaaaat aacgtagagc ggtaaccttg ccataaaggt
      241 cgagtttatt accatccttg ttatagactt cggcagcgtg tgctacgttc gcagctaaca
      301 aaccagttac aaccaccgca actattttta atttcattta acagaatcct tttaattatc
      361 gtcaaacgta ttttctaaac gaattttaaa cggcgtcatt tataaatgac atactgtttt
      421 taaaatagaa tggtgattaa aaacaatgag aaaaatagat gagataatat tatttatcga
      481 tatgtgatcg aagtcgaaat gagatataag gtgaattact ggtatttgaa atttattttt
      541 taatattgtc ggaatttatc tgattaactg ccgggccgta gacccggcaa ttattttaac
      601 cacgacgtgt cgccagccag cagagcaggg aaccgccgca gaccattagc gcgccttgcc
      661 agaatgagaa cgacagcggg gcgctgagca gcacggctgc aagcgctgag gaaagtacgg
      721 gcgtaaaata cgagcctacc gccattatgg tgacattgcc atgcaatata ccgacattcc
      781 atgcagcata agcaaatcct aaggtaaatg ccgcagagat gagtttaatc atgacgggcg
      841 tgctaaatac catttctggt tgttgcgtaa gaaaatagta aacccacaga cttgctcccg
      901 ttagcaggac aaaaacggta attccgttaa atccgcgtgc gtatttattc gttactgtgc
      961 aataggctgc ccagataaac gcaccaataa acgccaggaa ataactcaat gggctggtgg
     1021 tgatattatt gatgatttca tcataatgta acccattctc accgcctaac acccagcaga
     1081 cgccgaccag ggctaataat aatcctggta caatcagcca gttggttttc tgaccattaa
     1141 acagaatggc aaagagaatt gtcaggctgg gccacagata gttcaccata cccgcttcaa
     1201 tcgcctgatg acgggtcgcc gcatacccta aggaaagcgc cagacagatt tcatagctga
     1261 cgaataacag actcccggcg agtaaatagc ctttcggaat ctgccgaata cgcggaaatc
     1321 caaccgtgaa gattaacagc agcccgctta atgaatagat agcagctgcg ccgccgaccg
     1381 ggccgagccc ctcgctgaca ccgcgaatca atcctaccat cgtgctccac aggacgatcg
     1441 ctattagccc tatgagcgtt gctttttgtc gtgtcatgct cgctgttttg tctctcttgc
     1501 cgttaaaaat taagctgaat tttatagcat ttttttaact ggcctgtcag gcagtggtgc
     1561 gtttttctac cgctattgag gtaggtcaat ttgcgcaggc ggattatttt gtggcaaaca
     1621 cgtgttcttt ttgatttcgc gcaaaaagat tcagaatttt actgttagtt tcctcgcgca
     1681 gtaatatccc tgaaaaaaga ggaaagcaat ggacgtcagt cgcagacaat tttttaaaat
     1741 ctgcgcgggc ggtatggctg gaacaacggt agcggcattg ggctttgccc ctaagcaagc
     1801 actggctcag gcgcgaaact acaaattatt acgcgctaaa gagatccgta acacctgcac
     1861 atactgttcc gtaggttgcg ggctattgat gtatagcctg ggtgatggcg cgaaaaacgc
     1921 cagagaagcg atttatcaca ttgaaggtga cccggatcat ccggtaagcc gtggtgcgct
     1981 gtgcccaaaa ggggccggtt tgctggatta cgtcaacagc gaaaaccgtc tgcgctaccc
     2041 ggaatatcgt gcgccaggtt ctgacaaatg gcagcgcatt agctgggaag aagcattctc
     2101 ccgtattgca aagctgatga aagctgaccg tgacgctaac tttattgaaa agaacgagca
     2161 gggcgtaacg gtaaaccgtt ggctttctac cggtatgctg tgtgcctccg gtgccagcaa
     2221 cgaaaccggg atgctgacac agaaatttgc ccgctccctc gggatgctgg cggtagacaa
     2281 ccaggcgcgc gtctgacacg gaccaacggt agcaagtctt gctccaacat ttggtcgcgg
     2341 tgcgatgacc aaccactggg tggatatcaa aaacgctaac gtcgtaatgg taatgggcgg
     2401 taacgctgct gaagcgcatc ccgtcggttt ccgctgggcg atggaagcga aaaacaacaa
     2461 cgatgcaacc ttgatcgctg tcgatcctcg ttttacgcgt accgcttctg tggcggatat
     2521 ttacgcacct attcgttccg gtacggacat tacgttcctg tctggcgttt tgcgctacct
     2581 gatcgaaaac aacaaaatca acgccgaata cgttaaacat tacaccaacg ccagcctgct
     2641 ggtgcgtgat gattttgctt tcgaagacgg cctgttcagc ggctacgacg ctgaaaaacg
     2701 ccagtatgat aagtcgtcct ggaactatca gttcgatgaa aacggctatg caaaacgcga
     2761 tgaaacactg actcatccgc gctgtgtgtg gaacctgctg aaagcgcacg tttcccgcta
     2821 cacgccggac gtcgttgaaa acatctgcgg tacgccaaaa gccgacttcc tgaaagtgtg
     2881 tgaagtgctg gcctccacca gcgcaccgga tcgcacaacc accttcctgt acgcgctggg
     2941 ctggacgcag cacaccgtgg gtgcgcagaa catccgtact atggcgatga tccagttgct
     3001 gctcggtaac atgggtatgg ccggtggcgg cgtgaacgca ttgcgtggtc actccaacat
     3061 tcaggggctg actgacttag gtctgctctc taccagcctg ccaggttatc tgacgcttcc
     3121 gtcagaaaaa caggttgatt tgcagtcgta tctggaagcg aacacgccga aagcgacgct
     3181 ggctggtcag gtgaactact ggagcaacta tccgaagttc ttcgttagcc tgatgaaatc
     3241 tttctatggc gatgccgcgc agaaagagaa caactggggc tatgactggc tgccgaagtg
     3301 ggaccagacc tacgacgtca tcaagtattt caacatgatg gacgaaggca aagtcaccgg
     3361 ttatttctgc cagggcttta acccggttgc gtccttcccg gacaaaaaca aagtggtgag
     3421 ctgcctgagc aagctgaagt acatggtggt tatcgatccg ctggtgactg aaacctctac
     3481 cttctggcag aaccacggcg agtcgaacga tgtcgatccg gcgtctattc agactgaagt
     3541 attccgtctg ccttcgacct gctttgccga agaagatggt tctatcgcta actccggtcg
     3601 ctggttgcag tggcactgga aaggtcagga cgcgccgggc gaagcgcgta acgacggcga
     3661 aattctggcg ggtatctacc atcatctgcg cgagctgtac caggccgaag gtggtaaagg
     3721 cgtagaaccg ctgatgaaga tgagctggaa ctacaagcag ccgcacgaac cgcaatctga
     3781 cgaagtggct aaagagaaca acggctacgc gctggaagat ctctatgacg ctaatggcgt
     3841 gcttattgcg aagaaaggtc agttgctgag tagctttgcg catctgcgtg atgacggtac
     3901 aaccgcatct tcttgctgga tctacaccgg tagctggaca gagcagggca accagatggc
     3961 taaccgcgat aactccgacc cgtccggtct ggggaatacg ctgggatggg cctgggcgtg
     4021 gccgctcaac cgtcgcgtgc tgtacaaccg tgcttcggcg gatatcaacg gtaaaccgtg
     4081 ggatccgaaa cggatgctga tccagtggaa cggcagcaag tggacgggta acgatattcc
     4141 tgacttcggc aatgccgcac cgggtacgcc aaccgggccg tttatcatgc agccggaagg
     4201 gatgggacgc ctgtttgcca tcaacaaaat ggcggaaggt ccgttcccgg aacactacga
     4261 gccgattgaa acgccgctgg gcactaaccc gctgcatccg aacgtggtgt ctaacccggt
     4321 tgttcgtctg tatgaacaag acgcgctgcg gatgggtaaa aaagagcagt tcccgtatgt
     4381 gggtacgacc tatcgtctga ccgagcactt ccacacctgg accaagcacg cattgctcaa
     4441 cgcaattgct cagccggaac agtttgtgga aatcagcgaa acgctggcgg cggcgaaagg
     4501 cattaataat ggcgagcgtg tcactgtctc cagcaagcgt ggctttatcc gcgcggtggc
     4561 tgtggtaacg cgtcgtctga aaccgctgaa tgtaaatggt cagcaggttg aaacggtggg
     4621 tattccaatc cactggggct ttgagggtgt cgcgcgtaaa ggttatatcg ctaacactct
     4681 gacgccgaat gtcggtgatg caaactcgca aacgccggaa tataaagcgt tcttagtcaa
     4741 catcgagaag gcgtaagggg gcgaacagat ggctatggaa acgcaggaca ttatcaaaag
     4801 gtccgcaact aactccatca cgccgccttc tcaggtgcgt gattacaaag cagaagtcgc
     4861 aaaacttatc gacgtttcca cctgtatcgg ctgtaaagcc tgtcaggtgg cgtgttcgga
     4921 gtggaacgac atccgtgatg aagtggggca ctgcgtcggg gtttacgata accccgccga
     4981 tctgagcgcc aagtcctgga cggtgatgcg ctttagcgaa accgaacaga acggcaagct
     5041 ggagtggctg atccgtaaag acggctgtat gcactgtgaa gatcccggct gcctgaaggc
     5101 gtgcccgtct gctggtgcaa tcattcagta cgctaacggg attgtcgatt tccagtcgga
     5161 aaactgcatc ggctgtggtt actgcattgc cgggtgtccg tttaatattc cgcgcctcaa
     5221 caaagaggat aaccgggtat ataaatgcac gctctgcgtc gatcgcgtca gcgtcggcca
     5281 ggaaccggct tgtgtgaaaa cctgtccgac cggggctatc cacttcggca ccaagaagga
     5341 gatgctggag ctggcggaac agcgcgtggc gaaactgaaa gcgcgtggtt acgaacatgc
     5401 tggcgtctac aacccggaag gggtcggtgg tacgcacgtt atgtacgtgc tgcatcacgc
     5461 cgatcagccg gagctgtatc acggtctgcc gaaagatccg aagatcgaca cctcggtaag
     5521 cctgtggaaa ggcgcgttga aaccgctggc agcggctggc tttattgcca cttttgccgg
     5581 gttgattttc cactacatcg gtattggccc gaataaggaa gtggacgatg acgaggagga
     5641 tcatcatgag taagtcgaaa atgattgtgc gcaccaaatt tattgatcgc gtctgtcact
     5701 ggaccgtggt gatttgcttc ttcctggtgg cgctgtccgg gatttcgttc ttcttcccga
     5761 cgctgcaatg gctgacgcaa accttcggta cgccgcagat gggacgcatt ttgcacccgt
     5821 tcttcggcat tgcgattttc gtcgcgctga tgtttatgtt tgtgcgtttt gtgcatcaca
     5881 acatcccgga taagaaagat attccgtggc tattgaacat tgtcgaagta ttaaaaggca
     5941 atgagcacaa agtggcggat gttggtaagt acaacgccgg gcaaaagatg atgttctggt
     6001 cgatcatgag catgattttc gtgctgttgg tgaccggggt gattatctgg cgtccgtact
     6061 ttgcgcagta cttcccgatg caggttgttc gctacagcct gctgatccac gcggctgccg
     6121 gtatcatcct gatccacgcc atcctgatcc atatgtatat ggcattctgg gtgaaaggat
     6181 cgattaaagg gatgatcgaa gggaaggtaa gccgtcgctg ggcgaagaaa caccatccgc
     6241 gctggtatcg tgaaatcgag aaggcagaag cgaaaaaaga gagtgaagaa gggatataat
     6301 ctcttttgaa ctttaagctg aaaatggcgc tttaagaagc gccatttttt tattgcgtaa
     6361 ccagacggcg cagtcgcgac acatccaccg ttttttcagc ctctgccaga ctccaggcgt
     6421 tttgcagatt cagccacatt tgcggcgaac tgccgatcac cacggaaagt ttaattgcca
     6481 tttctggcgt caaagctgct tttccggtca gcaatcggct tgccgttgag ggcgcaattt
     6541 ccattgctct ggcaaactcg cgcaggctga cattaagttc gtccagtgat tcctgaataa
     6601 tgtcccccgg gcggggatga ttggccattt tcattagtgt ctttactctc agtagcgttc
     6661 cttctctggg aacactatca ttattgttcc ttcctacgca acaataatcc atttagccat
     6721 ttcatgttgg agagtgtgcg atgctgcgac ccgaacatgg gagtcgcagc aaagacctct
     6781 tagtgacgga aatcaatcac catacggcca cggattttgc cttcttccat ctcggtaaag
     6841 atggtgttga tgtccgctaa cggacgcagg gcgactttcg gcaccacttt gccttcggcg
     6901 gcaaactgga aggcttcggt tagatcctgg cgcgtgccga ccagcgaacc gaccacttca
     6961 atgccatcca gcacaagacg tgggatatcc aggctcatag actccggcgg cagaccgaca
     7021 gccacaacac gaccgcctgc acggacagca tcaactgccg agttaaacgc agctttagct
     7081 actgctgtta ccaccgcagc gtgagcgcca ccggttttct cctgcacaat ttaggcggcg
     7141 tcttcggtgc gtgagttaat cgctaaatct gcacccattt cggttgccag ttttaactgc
     7201 tcatcattga catcaatggc gatcactttg gcgttaaaga cattcttcgc gtattgcagg
     7261 gcgaggttac ccagaccgcc aagaccctag atagcaatcc actgaccggg acgaattttt
     7321 gacagtttaa cggctttgta ggtggtgacc cccgcacagg taatgctgct ggccgccgcc
     7381 gagtccagac catctggcac ttttaccgcg taatcggcga ccacgatgca ctcttccgcc
     7441 atcccgccat caacgctgta tccggcattt ttaactgaac ggcagagcgt ttcgttacca
     7501 ctgttacagt attcgcaatg accgcatcct tcgtaaaacc acgctacgct ggcacgatcg
     7561 cctggtttta atgaggtgac acctgggcca acttctgcca ccacaccgat gccttcatgg
     7621 cccagaatta ccccagtttt gtcaccaaaa tcgccattct taacatgaag atcggtatga
     7681 catacaccac aacactccat tttcagcagg gcttcgccat gtttcagtga gcgcagtgtt
     7741 ttatccgtaa cgtcaacatg atgatccttc gtaacaactg cagccttcat agttcctcct
     7801 tttcggatga tgttctgcat agcaggtgag gcaaatgaga tttattcgcc actacccagt
     7861 atggatgaga tctgaaaaag ggagagggaa atagcccggt agccttcact accgggcgca
     7921 ggcttagatg gaggtacggc ggtagtcgcg gtattcggct tgccagaaat tatcgtcaat
     7981 ggcttgttgc aaagcttcag cagacgtttt caccgccacg ccttgctgct gcgccatttt
     8041 gccaaccgca aacgcaattg cgcgggagac tttctgaata tctttcagtt ccggtagtac
     8101 cagaccttcg ccgttcagca ccagcggcga atactgagca agcgtttcac ttgctgacat
     8161 cagcatctca tcggtgatac gtgacgcgcc ggaagcaata acacccagcc cgatgccagg
     8221 gaaaataaag gcgttgttac actgggcgat agggtagatt ttatctttcc ataccactgg
     8281 attaaacggg ctgccagtgg cgaccagcgc gttaccttcg gtccaggcga taatgtcctg
     8341 cggtgtggct tccacgcgtg acgtcgggtt agacagcggc atcacgatcg gacgcggaca
     8401 gtgtttatgc atctcacgga tgatctcttc cgtaaacagc ccggtctgtc ctgagacgcc
     8461 aatcagaata tctggtttta cattgcgcac cacatccagc agtgacagca catcgctgtc
     8521 ggtatcccag tcactgaggt tttcgcgttt ctgcaccagt ttggtctgga aaggcagcag
     8581 gttcggcatc ttgtccgtca gcaggccaaa gcgatcgacc ataaagactt tctgccgcgc
     8641 cgcttcctcg cttaatcctt cgcgctgggt ctgggcgatg atcatttcgg caatgccgca
     8701 tcccgctgaa cctgcaccaa ggaagacgat ttttttctcg cttaactgac cacctgccgc
     8761 tcggcttgct gcgatcagtg tgccgactgt taccgccgca gtgccctgaa tgtcatcgtt
     8821 aaaagaacaa atttcattgc gatagcggtt aagtaacggc atcgcatttt tttgagcaaa
     8881 gtcttcaaac tgcaacagca cgtctggcca gcgttgtttc acagcctgga taaattcatc
     8941 aacgaattca tagtattcgt cgtcaatgat acgcggatta cgccagccca tatacagcgg
     9001 atcgttaagc agctgttggt tgttcgttcc gacatccagc accaccggaa gggtatacgc
     9061 cgggctgatg ccgccacagg cggtatagag cgacagttta ccgatcggaa tgcccatccc
     9121 gccgatgccc tggtcaccaa gccccagaat acgttcaccg tcagtcacca caatcacttt
     9181 gatattatgg ttcggtacgt tttgcagaat atcgtccata ttgtggcggt tctggtaaga
     9241 gataaacacg ccgcgtgaac ggcggtagat ctcagaaaaa cgctcacagg ctgcgccgac
     9301 ggttggggta taaataacag gcatcatctc atcaagatga ttgtttacca gacggtagaa
     9361 gagggtttcg ttggtgtcct ggatgttacg caggtagatg tgtttgtcga tttcggtttt
     9421 gaatccctga tactggatcc atgctcgttc cgcttgttct tcgatggttt cgaccacttc
     9481 cggcagtaac cccagcaggt tgaagttacg gcgttcttcc atgctgaagg cactgccttt
     9541 attcaacaac ggaaattcca gcagtacagg gccagcgtaa gggatataaa gcgaacgctg
     9601 tttttttgtt tttggttcca tgtcactcac tcttttttga atatccatcc ctggcacgct
     9661 tttatcgtct ttgctttacc gccagggcgt cggccccaag tataaagcag ataaaaacaa
     9721 aaacaccatt gcgcaggcaa tggtgtttaa tcgtcattga ggactgatgg ttatgaatta
     9781 cttttcagcg gggcgttttc tgccggttgg gttatttacc acgctggatt tgtcaccttc
     9841 ggtaactatt ttgcgctggt tagaaatttt atggtccagt ccaagaatat gacgtgcctg
     9901 acggttcgat ttcattttaa ctcctcaatc ctgttgctag ttttaaggac aacatcgccg
     9961 tagcgaagaa acacgtgcta aacccctaaa ttaggttgcc gatcaagcat agcaccttaa
    10021 agcgtagggt gctggccact gaccacataa ttgatcgttt gctggtagat atcactgagg
    10081 atgtcgttat cagaagcttc cacccatttg gtcagctcca tgagaatgtc atcttcagtg
    10141 acaacaccgt gctctgcccg aaggccttgc tcaatggcat taaccagagc gggttctgct
    10201 gttgaatttt ctgcctgata ataagtaaac atagtgattc tccgtgtctg tgtatttatg
    10261 gtgtctgcta cggatcgcag atttataaag cacattcagc atggcaaata tttgccgctt
    10321 tgttgttaag attagtcctg gttgatgatt tttatatttt aacctcatga tattgatagg
    10381 tattattatt gtggtgagtt gattaatatt aatgcaatat ctcttgggtt atatattccc
    10441 ggttatctat tgtggaaatt taatttaagt gcagaagtaa tatttctgcc ggatttttat
    10501 tcggaatatc ctgcttatcc tcgtgctgtt tctcacgtag tctataattt cctttttaag
    10561 cccacatgag agcaacaatg acaatccata agaaaggtca ggcacactgg gaaggcgata
    10621 tcaaacgcgg gaagggaaca gtatccaccg agagtggcgt gctgaaccaa cagccgtatg
    10681 gatttaacac gcgttttgaa ggcgaaaaag gaactaaccc tgaagaactg attggcgcag
    10741 cgcatgccgc atgtttctca atggcgcttt cattcatgct gggggaagcg ggatttacgc
    10801 caacatcgat tgataccatc gccgatgtgt cgctggataa agtggatgcc ggttttgcga
    10861 ttacgaaaat cgcactgaag agtgaagttg cggtgccggg tattgatgcc tctacctttg
    10921 acggcatcat ccagaaagca aaagcaggat gcccggtctc tcaggtactg aaagcggaaa
    10981 ttacgctgga ttaccagttg aaatcgtaaa gcattgccgg atgacgtgtc aggcgcgtga
    11041 atgcctgatg cgttgttagc atctctacag cgcccgccag caccggagct cacgtccgtc
    11101 ctcccttatt gctaatgatt gccggacttc acatccgctg gttgcaaggg ggcaacgttc
    11161 gtagaaaaaa cagccttgcg gcaacgtgcg gtttcccggc agatccgttt tacgtaatgc
    11221 ccattcttcc tccagcggtt tatcaatggc ggggagggaa tccagcaata atcgggtgta
    11281 tggatgtgcc ggagccgtta gcacctgttg cgcgtcgccc agttctacaa tctgcccgag
    11341 atacatcacc gccacccgat cgctcatatg acgtatcacc gagacattgt gtgaaatcag
    11401 cacataggtc agcccgtgat tttcctgtag cgttaccagt aaattgagga tctgcgcctg
    11461 cacggagata tccagcgcag aggttggctc atcaagcaca atcacgtcag gctgcgaaga
    11521 gagtgctctg gcaatggcga tacgttgccg ctgcccgccg gagaacgcat gaggcaggcg
    11581 gtcgagatac tccggacgaa tacccacctg cacagccagc tcctctacca acgctcgccg
    11641 ctgttgttca ctactacgct tagctatcca gagcggttct gtgatgatgc gccacaccgg
    11701 taagcgcgga ttgagcgaag agagcgggtc ctgaaacacc atctgcataa tgcgttgtga
    11761 gccagaacgg atgtactgcc cgtggctcgg ttgcagcata cccattaaaa gctgtgcgag
    11821 ggtgcttttg ccgcagcctg actcgccgac gatccctaag gtttcaccac ggcggatctg
    11881 taaatcaata ccattaatcg catgaacatg ttccgtagtt ttaccaagcc agtttttacg
    11941 ggccgggaaa ttgatatgga cgccgcgtaa cgttaataac gtgtcagaca cttatgacct
    12001 cctgctgcgg ataccaacag gcgcagcgct ggttgttgtc accacacgct gtcagcgccg
    12061 ggacgttttc acactgtgca cctgccgcat agcaacgatc gcgaaaagcg cagccgtcag
    12121 gcaaatgggt gaggtttggt accgtccctg gaatggcggg taatagctgg cgtggtactc
    12181 catgttctgg tgcacattgg agcaaaccaa tggtatacgg atgccgggga tgatggataa
    12241 cgtctgccct cacgccgctt tcaattacgc ttccggcata catcacgtaa acgctatcgc
    12301 acagttgcga caccacggcc atatcatggc tgatgaacag aaccgcagtt ccactggcgc
    12361 gggctttatg tttaagcaaa cgcagtacct gcaactgcac cgtgacgtcc agcgccgtag
    12421 tcggttcgtc ggcaataatc aattgcggct cgcaggagaa tgccagcgca atcattaccc
    12481 gctggcgcat accacctgaa agctcaaacg gatagcgcga cataacttcc acggcatccg
    12541 ggatttgcat ctcttccagc aggacaatcg ctttagctct ggcttcccga cgacttattg
    12601 gttgatgatg gcggatcacg tccatcatct gaagacctat tcgacgtgtc ggattgaggg
    12661 cggtcatcgg ttcctgaaag atcatcgcca ctcgtgcgcc gcgccactga cgaagctgtt
    12721 tttcccgagc gttaagaaca tcgtctccca gcagtgaaat ctgcccccga tgtacgcaat
    12781 aactgcccgt cggtagcaga cgcataatca gcattgcggt gactgattta cctgagccgg
    12841 attctcccac cagaccgaca atttcaccgc ggttaatctg caaggacaca ttgttgagcg
    12901 cgtgaacatc accgttaaaa ccggggaaac tcaaatgcag ctgttgaatg tccagaacgg
    12961 gctgggtcat gactgctttc ctcctgcttt cgggtcaagc agatcgcgaa taccatcacc
    13021 aaagagatta aacccaacgg cggtaagcaa aatcgctgcc cctggaaatg cgcaatacca
    13081 ccactgatcg agtacatagt tgcgaccatt cgctaccatc gcgccccatt cagcactcgg
    13141 ttgttgagca cccaggccaa taaatcccaa cgtggcggcc attaaaatcg cgctaccgat
    13201 atccagcgat gcctgcacga tcagcggcgg cagggagtta cgtaaaatat gccagctgat
    13261 cagatgccaa cgcgacgcac caaaggtttt cgccgcctga acataggtat actggcgtac
    13321 aactaatgct tgcccccgcg ccaggcgcac ataaaaggga attcgcacaa tagcaatcgc
    13381 cagcatggcg ttaaataaac tcggcccgag agcggcagcc agtgccattg tcagtaccaa
    13441 cgaaggaatc gacagcataa tgtccatgat gcgcatgatg atggcgtctg cgcgtccgcc
    13501 aagcacaccg gatagacatc cgagtagcga accaatcata cccgcaatgg caaccacgac
    13561 taatccggcg agaattgatt gctgactgcc gaccagtacg cggctgaaca gatcgcgtcc
    13621 cacttcatcg gtgccaaacc agtgcgccgc agaaggcggc aaaaggcggg cggttaaatc
    13681 aatggcgttg ggatcatgcg tcgcaagcca cggtgaaaaa aacatcatca atagcattaa
    13741 tacaatgatg actgcgccgg tcacggtgag cggactgcct ttcagcatcc atatcagttt
    13801 tgcaccgtta aatcgcgttt gtttttgtgg gcgtacggcg gacgtttcct cgcttagcat
    13861 cattcaccac ctccacgtcc gatacgcgga tcaatccaca aatagagcaa atccaccacc
    13921 aggttgacca gcacataagc aaatgaaacc acgacggcaa agcccatcac tgccgggaag
    13981 tcgagcgcct gtattgatgt tactacccat gcacccattc cgggccaggc aaaaacggtt
    14041 tcggtgagca ctgcgccata caacaaatcg cccagcgcca gacccaatac ggtaatcgat
    14101 gggatcaacg cattgggtag cgcataacat aaaacgatat accagccggg caagccgctg
    14161 gcccgggcgg tacgaatgta gtcttcactc aattgttcca gcatcgctga gcggatctgg
    14221 cgagcgacaa ttcccaggtg aacgaacgcc agcgttaatg ccggtaagat gagatgttgc
    14281 aacgcattga agaagacttc accgttgcct tcaagcagcg catcgagcag ataaaagccg
    14341 gtaacgtgcg ttggtggatc cagccagtca tcaagccttc cgccgccggg aagaatttgc
    14401 agatgaccat aaaacagcac aatgacgccc agtccaagcc aaaacgcagg tgtggagatt
    14461 ccggtaatgg ccattattcg caccagatga tccagccagc gatttcgcca gactgcagag
    14521 agtatgccca gcgggatgcc aatcaggagt gccagcagca gggcacaaaa agccagttcc
    14581 agcgtcgccg ggaaaaatat acgcaactct tccaacaccg gacgcccggt acgaatggat
    14641 gttcccaggt caccatgaaa caggtcgctg acgtagcggt aaaactgtac atacagtggc
    14701 tggtccagtc ccagttgctg gcgaatattt tccacgatag catcgctggc gcggtcaccc
    14761 gccagtaacc gcgccggatc gccagggatc agatgcgaga taataaacgt aatcacgcag
    14821 acgcccgcca tcacgagcac cagcccccag cagcgttggc gtaaaatact ccagaaggtc
    14881 acgttattta ctcatggtat tgatattgaa gacctgttcc agcatgggat tgaacacaaa
    14941 gcctttcacc tctttgttca tcgccagttg gtagtttttc tggaacaggt acacataagc
    15001 agcgtcatca atgacgattt tctgtgcctg ctggtagtcc cgggtacgct gcgtctggtc
    15061 ggtggtcgca agcgcattgc gcagtaactt atcgacctca ctgttttcat agaacgagcg
    15121 gttacccggc agaccttttt tgtctgactc aaaccagtaa ttcataaaca tatacgggtc
    15181 ggcaaaatcc ggactccagt tgccaatcgc aatgtcgtaa tcacctttac ccactctgtc
    15241 gcgcatggtg gcgttcgcca gcttttccag cttcacattg atgcccagct tgttgagact
    15301 ggattgtgtc gccagagcaa taggctccca gttcggatcc ttatcggagt agagaaacgt
    15361 caggctggtg ggtttgctcg tcactttatc ccattcggct ttggctttcg tttcgtcatg
    15421 gttgtattgc attgccgtcg catcgtagcc ccacatgcct tccggaatcg ggccgcgcat
    15481 ctgttttccg ttaccactca gaatgccgtt aaccatgccc tgataatcgg tagaccagga
    15541 aatggcccga cgcagatccg cctgattaag aggcgctttg ctgttattca gatacaggta
    15601 ggtgacgcgc agtgacggat actctgccac attgactttg ttttcctgct tcagggcgtt
    15661 gagttgatcc accggcagcg catcggcaat gtcaatatca ccacgggaga gctgcaggcg
    15721 acgggaggca ctttcaccaa taatttttac cgatactcgc ttaaagttcg gtttattgcc
    15781 agggtaatgc ggatttggca ccagaactaa ttgctgacct ttttgccagc ttttcagcat
    15841 aaatggtccg gaaccggcgg tattttgcgc gaggaagccg cgggcatcat ccgctgcatg
    15901 ttcctttaac accgccggat tgataatgga tgcaccgtca ttcgccagcg tgtagaggaa
    15961 cggtgcgaat ggctggctaa gggtaaactt caccgtatgt tcgtcgggag catcaatctt
    16021 taaatctttg ggaaatgctt ctgctggccc ctggccgatt tttagtagcc gctcaaaaga
    16081 gagttttact gcttcggcag tgaccggtgt gccatcggca aatttagcat tgtccttcag
    16141 ggtgaacgtc cactcttttt gatcgtcaga cgctttccag ctacttgcca gatcgccttc
    16201 aacgtcggtt gagcctttat caccgtccgt tttgtactga accagccgct gataagacgg
    16261 gtaggtcacc gtccagtcgt tattatctat tgttaccgcc ggggcgaggg tttgtggatc
    16321 ggcggcctta ccaatcacca gcatatcttt cggtacggcg gcgtgcgcaa ccgggaaagt
    16381 tgtggcaagg acgagcgcga gcaatgtagg acgaaacggt atcgatctct tcatgacagt
    16441 ttcctgatta actgacgtgc tgtgtgccag gggatataaa acaggtgaat tgagtgggta
    16501 actcgctgcc tggggtaatt cgaagtgcca ccattcgctg gagataccga caaaaccgcc
    16561 accagtcata atcgcattca gcaacagccg attgcgctga gcggcgggcg ggacggaagg
    16621 gtgataggca tgggagcgct cgtgcatttc atcgaaccct gcgcccatat cgaggatgtt
    16681 cccgtgctca tcacgaagcg tcaggtcgat cgccgtgcca cggctgtgat tagaaccgac
    16741 cgtcacatca acaacatatt gcgggtctgg gcaggcttgc cacaacatcg cctgtgcttg
    16801 ttgtgggcga tacgcatcgt aaatcaccag ttgtaagcct gacagctggg cgatactgat
    16861 acttttcgcc agcgcggtaa tcgcatcctt gtgtagcaga caacgcgctt gctgataaat
    16921 agctttacct gtgatgttat cagcgcaggc gtatttcaat tcgatctcca gatcagggaa
    16981 gattacggct aaatcaacca gttcggtggt atccgacata aacggttcct gtataagaca
    17041 aaaattgctg cgctttccgc ttatgcagat ctcatgccat gccgggataa gcgccagaat
    17101 gctggcttaa agttatattt tttaactttt gatcaacatt tgtgcagcgt agtgcagttt
    17161 tggtgcaaga ggggaagtta aggaaggaat ctcccggaat cgtagatgaa atcacagtat
    17221 ttaagtgacg gtgtcacgtt aaatgaaaac ccgcgagtgc gggcgagagg aatttgtcag
    17281 attttcagcg gtaacacgct gctcatccag cctggaattt cttcggcggg tagggggcgg
    17341 gaaaagaaat atccctgaat aacgcgacag tggatcttgc gtagcatctc aaattgctct
    17401 ttggtttcga cgccttccgc cacgacggtt aaattgaggc tttgcccaat gctggtaatg
    17461 gcttcaagta aggcaaggat gcgtttttcg gtcagacaac gatcgacaaa acttttgtca
    17521 attttgattt ccgttaccgg aagactgact aagcgggata atccggaaaa gcccgtacca
    17581 aaatcatcta ccgataagcc cacgcccata tcacgcagga tctgaatgcg cttaaagatt
    17641 tcggtatcgt gttccatcat catgctttcc gtgatttcta ccgtcagctg gtggccgtca
    17701 ataccccagg cgtgcattgc atcagacacc tgattaggca gttgattact gcgaaagtgc
    17761 agtgccgaca agttaacgga aagcgccggg atatgaatat tctggctacg ccattctgct
    17821 aactgacggc aagcttccgc gatgacccag cgcccaatat tttcgatttc accaatctct
    17881 tctgcgagag gaataaaccg tgaagggggc acatgaccat gctggggatc gtgccagcgc
    17941 gcaagggctt cgatgccgta cagttcaccc gtttctgcga agatttgcgg ctggtaaaca
    18001 agtttcagtt ggttattgct aatcgcttct ttcagcgctg cgcctaaaac caaacgctct
    18061 tttaccattt cgttcatcgc cgggctgaag aactgccagc cgttaccgcc attcttgcga
    18121 atataatcta ttgcattgtg agcagtggag agtaggtaat cgtggttttt acccacgtcg
    18181 tagctgatgc caatactcaa ggtaagcggg aatggtttat cgtcaatcat tatcggcttg
    18241 ctgaccacat tccgtagctc atcggcgatc tgggtaatgt tactgacgtc gttttccagg
    18301 ctcacgagga caaactgcgt accttcgata cgacagagat actgatccgg tttgagtttt
    18361 ccacgaaagc gattgaccac ttccagcaat gcctgatcgg cccacgcata gccaaggcta
    18421 tcaatcacat cctgaatatg gtcaacaccg atgagataca ccaccggaga gacggctttg
    18481 tcgaccaggt catcgaggta attgtgctgg ttattgcgat ttggcagacc ggtcatcgga
    18541 tcaaattgga tgagttgttc aatatgctgg cggctttttt cctgttccag cgccagcgcg
    18601 gccatatgct gactgatatc tgccacgcgt tcgataaagg cgctggtttc tgctcctgac
    18661 gaggttttaa tttgcaggat ccccgcaggc gcgccatcac gctgacgaat ggtcgctgac
    18721 cagctttgcg cattttgaac ttctgcaccg tgcgaagatg acgcccagtg tatcggcatc
    18781 ccgttgcgca gtgcgaacag cgaaacatgc gattcgttga gaacagattc gatgttacga
    18841 caaatgattt cccccatttc atgaaatggc gggctgctgc acatggcggc gagaatattg
    18901 ccttcaagct ggcggatttg ccgttcttcg gtgatatccg agaaagtcat taccaggttc
    18961 tgcagatgcg cgagcacgtc ataaaccggg ctgatagagg ctttaatcca gattttttca
    19021 ccggtgcgcg tcaacagcag aaattcgtcc tgatcgcggg cggttttcca tagcaactgt
    19081 tgtaaacgaa tgcggttatc ggcagggaat tcaggaatgt tcaggagtgt atcgggctgc
    19141 ataccgctgg cttcgctaat gcagtaacca aacatttcgg taaatgcgcg attgcactgc
    19201 acaatatggc gttccggatc gaggacaatc accggtcggt cgagatggtc aacggcaata
    19261 atcaattgtc gggtctgttc tttttgcgcc atttctacgc tggcatcccg taccagcgcc
    19321 aggtaataaa ctttcccctc ggcgctcact ttcgatagcg caaaacgggt ccagatttta
    19381 ctgccgtctt ttttctccag ctgcagctcc cgactcatcc cctcgacgcg cgctttacca
    19441 ccttcacggt tatgacgaat gtattcagga tgcgcaggac gcaaatcccg cggaatcagc
    19501 atatcaatgt tattgccaat gacttcttca cgtttgtacc cccagagctt ctctgcggcg
    19561 gggttgaaaa acatcacttc atcattttcg ttaattaaca ccgcgcccat catattttgt
    19621 tcaagggcgg ggaaaaaaat gccatcggcg gcattttccg catcggttag cttcatgatt
    19681 acctctgcat cctggcgcat ctaaagactg gctttccaga gttcaacacg gtttctacct
    19741 cgtcttttgg cgatatacag agcttcatcg gctatttgaa tgaggcgctc atagtcagga
    19801 tgaccattaa acatggcggc accgatggaa agtgagaggg caatatcctc gccgtttgcg
    19861 gctttcagtt tggttttctc cacccgactg cgaatacgtt ctgcggtacg taacgtttcg
    19921 ttttcagaag cttcagtcaa aacaatgata aattcatcgc ccccgtagcg aaaaacataa
    19981 tcactactgc ggacgttgtc ataaaaggcc tgagagactt tacgcagaat ttcatcgcca
    20041 gtgttatggc cccacgtatc gttgatctct ttgaatttat caacgtcaat aatcagcact
    20101 gacagcggtg taccggtccg gttggcatgg gcaatttcgc gtttgaagat agtcggcagg
    20161 aagcggcggt taagtaattt cgtcagtaca tccataccga cttcgtggcg cgatacttct
    20221 tcaaacaatt cacgcagcaa ggtaataatt tgcgatacag tatttcttat ctgtaataaa
    20281 aatttcaccc gcagacttct gttattcaaa ttcctggtgt tacgcatggt ttgattgaat
    20341 ataccgtcga aatcctgaat cagacgggag atatgcctac ttcggcaatg ccactaaaat
    20401 aatgtcgacc tttatggtta aaccacaggc caaaatcagc ctggcttaaa ggcaaactac
    20461 tgcctaaatc agaatccagc aggattttat agataatatc tatttcccat gaaagtattg
    20521 aggctatttg ccgttctttt tcttcttcgg cgttttctag taacgagaag atacgatagt
    20581 tttcatcttc ctttgaggca ctactgtcac taaaggtaaa cgcgcgggtc atcacttcca
    20641 tagcgatatc aatactgtta atcgagaaat ggtagacctg aagtttttct gcggcagaat
    20701 aatccgaaga gaagattacc ggatagagga tttttttcag cacccgaaac cccatctcga
    20761 caatttctac cggaattcct atgcgggcat gcacttccgc gacggtatgc tggatttgta
    20821 ttagcctttc gacatcgtca acctgggcag aaagcacgtt aataatccag cgttccatcg
    20881 cacttttcaa ctgccgctca acttgttcat tactcaagaa ttcttcggca tgcgggtcga
    20941 tgcggacaat tcgataaaac tcgatactca gataatgagc atgcgcaacg gcaatttccg
    21001 cggctttagc acgaatgagt ggatctgcct gttcgacaag tccggtccac tcatctttca
    21061 ttcttttaaa atacatctcc ataattcaca cccttataag gctgggaaat cagacggaat
    21121 caaaacgcaa cgtgcgagat cgactaactg caccatattc tcctgaaata tgaagatata
    21181 ctgaaaagaa ataagcgact taggacagtt tcaatctacg ctactgttct tcagaagagt
    21241 atagcccatc gtaattattt ttcggtgaca gcgaatatcg tatggttttt catattcata
    21301 catttttatt agggatttat agctgtttaa ctaagtgtgg ttaattgcgt ggttaatttg
    21361 acttatgtaa gcatgattac tagtgggata tttcacaagg tgtaacaagc cggtgggtaa
    21421 agcaccggct tgttacaaag taagcatggg agtttaactg ccccaacgac tttgcagata
    21481 gctgaccgct tgttgagtct gcggtttatt cagatagtcc tcacggaaca agatggtgcc
    21541 gctaatttct ggtaccgcat cgttaagatc gagctgcttt ttcagttccg gtacgccgcc
    21601 gttaatcatc cagtctggct ctatctttga aggttcaccc actttataga aggcgatacc
    21661 gatatacagg cgggtcctgg tcggtttaac gacatccgcc caccattttg ccaacacgtc
    21721 ataacgcgcg gcactccgtg agaacggcca gtaaatttgg ggagcaatgt aatccagcaa
    21781 tccttgttcc acccatcgac gggtgtcagc gtaggattcg tcataggctg ccgcgcctcg
    21841 ggtatcggaa ccgagcggat cgtgtgatcg gttacgccac acgcctgccg ggctaacgcc
    21901 aaattcgact cccggcttaa tgcttttaat ggtgtgcgat acttttgtaa ttaactgctg
    21961 agtattgttg cgccgccagt ctgcttttga cgcaaatgcg cctccgtatt tacggtacgt
    22021 ttcgttatca tttagccgtg aacccggtga ctccgtatag aaatagtcgt caaactgcac
    22081 gccatctacc ggatagcggg aaaccacttc ggcgactatt gatgtgatcc agtcctgaac
    22141 ctcagggatg cccgggtcga ggacaaagcg atcgccagac gttctgatcc agtcacggtg
    22201 ttgcacatag acgctcgccg gttgttgaga cagagtgcta ttcagttccc tgatagtacc
    22261 gggcttcgta ttaaccgata cgcgataggg gttaaaccag gcgtgtactt tcatcccacg
    22321 cttgtgggct tcgtcgagca taaattgcag cggatcgtaa cccggatttt caccaatctt
    22381 accggtcata agatcggacc acggcaaaat ttttgatggc cacagggcgg taccgtccgg
    22441 cttgacctgg aaaaagaccg tgtttatacc gagacgttgc agatgatcca gtttgtcgat
    22501 catcgcctgt tgttgtacac gggcccggct ggtggggtta ctaatgttaa ccgaggaaac
    22561 cggtggccag tcgaggcgag acaccgtggc cagccagatg ccacgcatcg gttgtgacga
    22621 ttgttgcgtc gtggctggtg gctttgaacc tgctggtggt gtcaccatgg actctggagg
    22681 cgtgctttta caactacaca gtaaaagtgc cagtgcaacc agtatcgctg gtcttctaat
    22741 cgttaatttc ttgtttcggg agcagatatc catatgtgct ggtttccggt aaacagatgt
    22801 gcgctcattc tcgtacttat cccggttaag tcaatacgac agcaagcacg aaaaagggag
    22861 cgatgaatta tcgctccctt gtcttataac cattcagaca tggttagtgt ttcttgtcat
    22921 tcatcacaat atagtgtggt gaacgtgcac gcgggtgcag gaagaagtga ccttttggtg
    22981 cgttctgact gttgattggc tccagagtga cgccggtatt cgctttgcct ttacgatcat
    23041 gaacagcata gagaataaag ggcagggcaa gtaccaccag gaaactaacg accagtaatt
    23101 caacatacat atcggtagag tcaccctgga tgttatccgg cggcaggaag gaaacaataa
    23161 acgccataat tgaagtcagc agaccgacaa ttgccacgac cagtttcacc cctttaccac
    23221 cagggatatt aaatgtgcgt tttaagtcag gatgtttaag aaccaacaca atgtagccaa
    23281 taaacagcat gaaataagca cacagataaa tcaccaccgt cagcgccagt gcgatcagga
    23341 aggacatgtt gttaccgcca ccggtattgg tgaggatgat caacgcgata gaagtaatca
    23401 ccagctgcga aatgaccagc gttaccggta cgccattttt gttcatttta gcgaatgccg
    23461 ctggcagcag gtttttctgc gctgtgacat acatcccgcg agaaggacca acaatccagg
    23521 aggcgatttc cgccagaaca cccagcaaca gcagtgcgga gatcacgcga accgtccact
    23581 caatttccgg tgccacatgg gacatcagaa cggtaaaggt ttgcattacc cctgcggaga
    23641 ggttgatttc attacccgga atgaccatcg caatagataa accgccaaca gagcttaagc
    23701 agattgccgc caccatcagc agtaacatag ccagtggata gtcgcgcccg gggttgctca
    23761 tttcattgac gtgagttgcg gaagcttcta cgcccatata actcaaaatg aaggcaacaa
    23821 atacaaccag tgtgcccact ttagagaagt cagggaagaa ggtcttcgaa tccatttcga
    23881 tagcaacggg ggcaccggag tgcagataaa tagccgctaa tgcgatcaaa ataaatgcag
    23941 gtaacaggat accggcgaag aagccaactt tagcaattcg cgccgtgtat ttcgtgccac
    24001 caaactgcgt taatgccagc gcccaaagaa tgatgagtgc tgcaatagtt ttggtaatgg
    24061 ggtcttcatt cagcgctggc catttcagga tgtaggagag tgcccctaac acgaaataga
    24121 gcatcggaat aaaaccaatg gcgatttgca gatagccaaa tgagatcgct gcaaatcccc
    24181 atctcggccc cagagtattt gatacccagg cgaagacacc accttcttcc cagccgtcga
    24241 cggtggccat ttccgcagca caaagtccca cgggaataaa ccataaaatc ccgcctaata
    24301 gcaggaagaa gactaatgaa aagcccgatg ttgcgaaggt agggtattca taaacagcca
    24361 ttaccatcga tgccgttatg gcaaagaatc cgagtaatgt gagctgctta gctttacctg
    24421 tctgtactga tgtagccata ttatccccct aaaacggtat tcctgtcgga accgcacctg
    24481 ttttgttctg aagcgtattc agaacaatat tttccgttgc taatgccagt gaacagactt
    24541 tggaaattgt cccgaaacgg gttcgtttcg ggacaccgtt accgttaaac gttatcaggt
    24601 atgtttaaag ctgttctgct gggcaatacc ctgcagtttc gggtgatcgc tgagatattt
    24661 cagggaggct ttgtagtctt ccagcaacag ttcagcaaag tccatttcga agccgcgac
//
LOCUS       DADLFA010000047        24084 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000047 DADLFA010000000
VERSION     DADLFA010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24084
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.047"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            322..549
                     /gene="feoA"
                     /locus_tag="JQH65_001586"
     CDS             322..549
                     /gene="feoA"
                     /locus_tag="JQH65_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HAY6139044.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            566..2887
                     /gene="feoB"
                     /locus_tag="JQH65_001587"
     CDS             566..2887
                     /gene="feoB"
                     /locus_tag="JQH65_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HAY6139045.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAIDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            2887..3123
                     /gene="feoC"
                     /locus_tag="JQH65_001588"
     CDS             2887..3123
                     /gene="feoC"
                     /locus_tag="JQH65_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HAY6139046.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            3326..4204
                     /gene="rpnA"
                     /locus_tag="JQH65_001589"
     CDS             3326..4204
                     /gene="rpnA"
                     /locus_tag="JQH65_001589"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HAY6139047.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMGCLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLTDI
                     MRFTGVSEEELAAASQ"
     gene            complement(4233..5003)
                     /gene="bioH"
                     /locus_tag="JQH65_001590"
     CDS             complement(4233..5003)
                     /gene="bioH"
                     /locus_tag="JQH65_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HAY6139048.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSQGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDDWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPRSKSYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            5041..5724
                     /gene="gntX"
                     /locus_tag="JQH65_001591"
     CDS             5041..5724
                     /gene="gntX"
                     /locus_tag="JQH65_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HAY6139049.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCRWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            5783..6358
                     /gene="nfuA"
                     /locus_tag="JQH65_001592"
     CDS             5783..6358
                     /gene="nfuA"
                     /locus_tag="JQH65_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HAY6139050.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            6719..8035
                     /gene="gntT"
                     /locus_tag="JQH65_001593"
     CDS             6719..8035
                     /gene="gntT"
                     /locus_tag="JQH65_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HAY6139051.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(8080..10164)
                     /gene="malQ"
                     /locus_tag="JQH65_001594"
     CDS             complement(8080..10164)
                     /gene="malQ"
                     /locus_tag="JQH65_001594"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HAY6139052.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLETMFADDGVNKLLKD
                     LDRRRRAAAKKQ"
     gene            complement(10174..12567)
                     /gene="malP"
                     /locus_tag="JQH65_001595"
     CDS             complement(10174..12567)
                     /gene="malP"
                     /locus_tag="JQH65_001595"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HAY6139053.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQEVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATIGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKLLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLQDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            13179..15909
                     /gene="malT"
                     /locus_tag="JQH65_001596"
                     /pseudo
     CDS             13179..15909
                     /gene="malT"
                     /locus_tag="JQH65_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(15952..16968)
                     /gene="rtcA"
                     /locus_tag="JQH65_001597"
     CDS             complement(15952..16968)
                     /gene="rtcA"
                     /locus_tag="JQH65_001597"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HAY6139054.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGAVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIEIDGVTR
                     VSIE"
     gene            complement(16972..18198)
                     /locus_tag="JQH65_001598"
     CDS             complement(16972..18198)
                     /locus_tag="JQH65_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HAY6139055.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKDSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAEDLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIKICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDVMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMTAQSD
                     LVEVIYTLRQVVCVKG"
     gene            18387..19985
                     /locus_tag="JQH65_001599"
     CDS             18387..19985
                     /locus_tag="JQH65_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-dependent transcriptional regulator"
                     /protein_id="HAY6139056.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWHPTLCLCQQESLVIDRL
                     ELLHDARSCSLFETLKRDIASVSPETEVVSIEIELHNPWDFEEVYACLHDFARGYKFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQTSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFFDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSATGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(19967..20725)
                     /locus_tag="JQH65_001600"
     CDS             complement(19967..20725)
                     /locus_tag="JQH65_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HAY6139057.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(20742..21572)
                     /gene="glpG"
                     /locus_tag="JQH65_001601"
     CDS             complement(20742..21572)
                     /gene="glpG"
                     /locus_tag="JQH65_001601"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HAY6139058.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVVMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIIAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(21617..21943)
                     /gene="glpE"
                     /locus_tag="JQH65_001602"
     CDS             complement(21617..21943)
                     /gene="glpE"
                     /locus_tag="JQH65_001602"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HAY6139059.1"
                     /translation="MDLFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            22133..23638
                     /gene="glpD"
                     /locus_tag="JQH65_001603"
     CDS             22133..23638
                     /gene="glpD"
                     /locus_tag="JQH65_001603"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HAY6139060.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HQPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(23690..>24084)
                     /locus_tag="JQH65_001604"
     CDS             complement(23690..>24084)
                     /locus_tag="JQH65_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6139061.1"
                     /translation="VMAGIGWQFSRRTPPELKNRLHCWLVFSPVYFWLILSNFILYIS
                     PEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGFALGYYCHWCRKNR
                     NQRKKWGDWVTCLSLAILAQGPLFNYLQ"
BASE COUNT         5660 a         6559 c         6092 g         5773 t
ORIGIN      
        1 caaactcata atatattccg gcaatattta tcacttcatt aacaactgaa accttaatta
       61 aacattagcc agtccgggta attcactatt cgaattatat tttcgctgcg atataacctt
      121 gagccacatc aacattgagt cagattatta ttcaaaccaa cattcgcaca cattttaagt
      181 attgctgata gaaaccattc tcattatcat tgtgttattg attatttaat ctctcctttg
      241 ttggcaaatc atctggtctc atgtcgctgt caaacgcccc atgaggtagt tatccagtta
      301 atgagaaaca agtaggcacc tatgcaatac actccagata ctgcgtggaa aatcactggc
      361 ttttcccgtg aaatcagccc ggcatatcgc caaaaactgc tttctcttgg catgttacct
      421 ggctcctctt ttaatgtggt gcgcgtcgct ccactcggcg accccattca tatcgaaacc
      481 cgtcgtgtga gcctggtatt acgcaaaaaa gatctggcct tattagaagt ggaagcggtt
      541 tcctgttaat acggtgataa caacaatgaa aaaattaacc ataggcttaa ttggtaatcc
      601 aaattctggc aagacaacct tatttaacca gctcactggc gcacgtcagc gtgtaggtaa
      661 ctgggctggc gttaccgtcg aacgtaaaga agggcaattc tccaccaccg atcatcaggt
      721 cacgctggtg gacctacccg gcacctattc tctgaccacc atctcatcgc agacctcgct
      781 cgatgagcaa atcgcctgtc actacatttt gagtggcgac gccgacctgc tgattaacgt
      841 ggtggatgcg tctaaccttg agcgtaacct gtacctgacg ctacaactgc tggaactcgg
      901 cattccctgc attgtggcac tgaacatgct cgacattgcc gagaagcaaa atattcgtat
      961 tgaaattgat gctctgtcgg cgcgtctggg ctgtccggtg atcccgctgg tttcaacccg
     1021 tggtcgcggt attgaagcgc tcaagctggc gattgatcgc tataaagcta acgagaatgt
     1081 ggaactggtg cattacgcac agccgctgct caacgaagca gattcactgg caaaagtgat
     1141 gccttccgac atcccgctga aacaacgtcg ctggctgggc ctgcaaatgc tggaaggcga
     1201 tatctacagc cgcgcctacg ccggtgaagc gtcgcagcat ctggatgccg ccctcgcccg
     1261 tctgcgtaat gagatggacg atccggcgct gcacattgcc gatgcgcgtt accagtgcat
     1321 tgctgccatc tgtgatgtgg taagcaacac cctgacggca gaacccagcc gtttcaccac
     1381 tgcggtagat aaaatcgtgc tcaaccgttt cctcggtctg ccgattttcc tctttgtgat
     1441 gtacctgatg ttcctgctgg ctatcaacat cggcggggcg ttacagccgc tgtttgacgt
     1501 cggctccgtg gcgctatttg tgcatggtat tcaatggatt ggctacacgc tccacttccc
     1561 ggactggctg actatcttcc tcgcccaggg cctgggtggc ggcattaaca ccgtgctgcc
     1621 actggtgccg cagattggca tgatgtacct gttcctctcc ttccttgagg actccgggta
     1681 tatggcgcgt gcggcgtttg tgatggaccg tctgatgcag gcgctgggct tgccggggaa
     1741 atcctttgtg ccgctgatcg tcggtttcgg ttgtaacgta ccgtcggtaa tgggtgcacg
     1801 tacgcttgat gcaccgcgtg aacgtctgat gaccatcatg atggcaccgt ttatgtcctg
     1861 cggcgcgcgt ctggctatct tcgcagtatt cgcggctgcc ttcttcgggc agaacggtgc
     1921 gctggcggtc ttctcgctgt atatgctggg tattgtgatg gcggtgctga ctggcctgat
     1981 gctcaagtac accatcatgc gcggtgaagc gacgccgttt gtcatggagt tgccggtcta
     2041 tcatgtacca cacgttaaaa gcctgattat ccagacctgg cagcgtctga aaggcttcgt
     2101 tctgcgtgct ggtaaagtga tcatcatcgt cagcattttc ctgagcgctt tcaacagctt
     2161 ctcgctgagc gggaaaatcg tcgataacat caacgactcg gcgctggcgt ccgtcagccg
     2221 ggtgatcacc ccggtcttca agccaattgg cgtgcatgaa gataactggc aggcaacggt
     2281 tggcctgttt acaggtgcca tggcgaaaga agtggtagtg ggtacgctca acaccctcta
     2341 caccgcagaa aatattcagg acgaagagtt caatccggca gagtttaacc tcggtgaaga
     2401 gctgttcagt gcgatagatg aaacctggca gagcctgaaa gacaccttca gccttagcgt
     2461 actgatgaac cccattgaag ccagcaaagg caacggcgaa atgggtaccg gggcgatggg
     2521 cgtgatggat cagaaattcg gtagcgcagc agcagcttac agctacctga ttttcgtcct
     2581 gctgtatgta ccatgtatct cggtgatggg agccatcgcc cgtgaatcaa gccgtggctg
     2641 gatgggcttc tccatcctgt gggggctgaa tatcgcttac tcgctggcaa cattgttcta
     2701 tcaggtcgcc agctacagtc agcacccaac ttacagcctg gtgtgcattc tggcggttat
     2761 cctgtttaac atcgtggtta tcggtctgct gcgccgcgcg cgtagccggg tggatatcga
     2821 actgctggca acccgcaagt cggtaagcag ttgctgcgca gccagcacca ccggtgattg
     2881 ccattaatgg cttcacttat tcaggtgcgc gatttgctgg cgttacgggg ccgtatggaa
     2941 gcggcccaga taagccagac attgaacact ccacagccaa tgattaacgc catgctgcaa
     3001 caactggaaa gtatgggcaa agccgtgcgg attcaggaag aacctgacgg ctgcctctct
     3061 ggcagttgta aaagctgccc ggaaggaaaa gcctgtctgc gcgagtggtg ggcgcttcgt
     3121 taaccttact ccatcgcctg ttttggaaag cccggtatgc gtctgcatcc gggctttttt
     3181 gcgtgcggct ttccataaaa atgcaactct tgcagcacgg cgtaagttcc tttgaaagca
     3241 tctcgcaggg atgaaaactc gctaatacac aggtgtggag tggcgcgtag agtcgcggca
     3301 ttcaaacaac aggtgaagga acgccatgag caaaaagcag agttccaccc cacacgatgc
     3361 gctgttcaaa ctctttttac gccaaccgga cacggctcgt gattttcttg cgtttcattt
     3421 accggcaccc attcacgcgc tttgtgatat gaaaaccctc aagctggagt cgagcagctt
     3481 tattgatgac gatctgcgtg aaagctattc cgatgtgctg tggtcggtga aaacggaaca
     3541 aggaccagga tacatctatt gtctgattga acatcaaagc acctcaaaca aactgatcgc
     3601 atttcgcatg atgcgttacg ctattgccgc aatgcaaaat caccttgatg ctggatacaa
     3661 aacgttgccg atggtggtgc cattgttgtt ttaccacggt attgaaagcc cctatcccta
     3721 ttcgctgtgt tggctggatt gtttcgccga tcccaaactg gcaaggcagc tttatgcctc
     3781 cgcatttccg ctgattgatg tcaccgtcat gcctgatgat gaaatcatgc agcaccgacg
     3841 tatggcgctg ctggagttaa ttcaaaaaca tattcgtcaa cgcgacctga tggggctagt
     3901 agagcaaatg ggctgcttat taagtagtgg atacgctaat gacagacaaa tcaaagggct
     3961 gtttaattac atactgcaaa ccggcgacgc ggtacgtttt aacgatttta tcgacggcgt
     4021 tgccgaacgt tcaccgaaac acaaggagag tttaatgaca attgcggaaa gattgcggca
     4081 ggagggggaa caatccaaag ccctgcatat agccaaaata atgcttgaat ccggagtccc
     4141 tcttacagac atcatgcgct ttaccggggt gtcagaagaa gagttggctg cggcgagcca
     4201 gtaaatttct gtctcgccat ttcaaaagtt gcctacaccc tctgcttcaa cgccaccagc
     4261 aggtgacaaa actcgaccgg atgcgaaata aacggcgcat gggccgcttt ggcgaagata
     4321 tatgatttgc tgcgaggcca gagtttatcc agcatcggca ccactttgcg cggcaccaga
     4381 ccatcgagat agccatacaa tcgcaaaaac ggcatgggca cgttttgcag cggctgacgg
     4441 agatcaaccg ttttcaggat ttccagcccg ccattaagca cgtcaacctc cggcatcggt
     4501 aacgccagaa cggttttctt caacgcccgc gcatcctggc gcgccgtttc agtccccatg
     4561 gtttgtaacg ccaggaaccg ctccactgta cgctgaaaat catcactgag ttgctgctga
     4621 aatcccgcca gcacgtccgg ttttatcccc ggccaatcgt cacgagcact aaaacaaggt
     4681 gacgacgcca cggtgaccag cgcctgaaca cgctcgggat gggttaacgc aatctggctt
     4741 gccaccagcc cgcccagact ccagcctaac caaatggctt tatcaggtgc ctgttgcagc
     4801 acggcttcgg ccatatcagc aagtgacagc gcaccaaatc cctggctgcg cccgaagccg
     4861 ggcagatcaa caaggtgcag cgtaaaatgc gagctaagtt cctcgtcaat gcaacgccac
     4921 acttcggcat tcagtcccca tccgtgcagc agcacaagat gaacattccc ctgacctttg
     4981 gtctgccacc agatgttatt catccgctat tgttctcttt tgacttacaa ggatgaacat
     5041 atgctaacag taccgggatt atgctggcta tgccgaatgc cgctggcgtt aggtcattgg
     5101 gggatttgtt cggtctgctc acgcgccgcc cgtacagata aaacgttatg cccacaatgt
     5161 ggattacccg ccacacactc acatcttccc tgcggtcgct gcctgcaaaa accaccgccc
     5221 tggcaaagac tggtcacggt tgccgactat gcgccgccgt taagtccgct tatccaccag
     5281 cttaaatttt cccggcgcag tgaaatcgcc agcgccctgt cacgtctgtt actattggaa
     5341 gtcttacacg ctcgtcgcac caccgggttg caattgccgg atcgcatcat cagcgttccg
     5401 ttatggcagc ggcgtcactg gcgtcgggga tttaatcaga gcgatttgct gtgtcagccg
     5461 ttatcacgct ggttgcactg ccgatgggat agcgaagccg tcacacgaac acgggccact
     5521 gcgacccagc attttctcag tgcccggcta cgcaagcgca acctgaaaaa tgcctttcgt
     5581 cttgaattgc ccgtgcaagg tcgccatatg gtgattgtgg atgatgtcgt taccaccgga
     5641 agtaccgtcg cagagattgc gcagttgctt ttacgcaatg gtgcggcgac tgtccaggtc
     5701 tggtgccttt gtcgaacctt gtagagcctc gatgataggc gtattataac caactaaaat
     5761 agtcaactat taggccatta ctatgatccg tatttccgat gctgcacaag cgcactttgc
     5821 caaactgctg gcaaaccagg aagaagggac acaaatccgc gtatttgtga ttaaccctgg
     5881 cacgcctaac gctgaatgtg gtgtttctta ttgtccgccg gatgccgtgg aagccaccga
     5941 cactgccctg aaatttgacc tgctgaccgc gtatgttgat gagttaagcg ctccttacct
     6001 ggaagatgca gagatcgatt ttgttaccga ccagttgggt tctcagctaa cactgaaagc
     6061 gcctaacgcc aaaatgcgta aagtggcaga cgatgcacca ctgatggagc gcgtggagta
     6121 tatgctgcag tcgcagatca acccacagct tgctggtcac ggtggccgcg tttcactgat
     6181 ggaaatcacc gaagacggtt acgccattct gcaatttggc ggcggctgta acggttgttc
     6241 catggtcgat gtgacgctga aagaagggat cgagaagcag ctgctaaacg aattcccgga
     6301 gctgaaaggt gtgcgcgatc tcaccgaaca ccagcgcggc gaacactcct actactaagt
     6361 tatcccctct tttgtagatt gcccgatgcg acgctaaagc gtcatttcgg gcctacgttg
     6421 aacatgtgcc agacgttggc gcagcattgc tttggcaaat ccctcccttc gtttctccgc
     6481 gataatatga ccaacctctc ataatttaaa tttactccgc tctggtgatt ctcaaacgcc
     6541 agatgttacc ggtatcattc acatgggtac caaacatact cctgacatct gactacaata
     6601 attagtttta gtgggtatca gtcgtggtgc cgcaatatct ctgttcccga ttgggataat
     6661 tagagtttgt cgtcagaaaa ttgacgttac ccataacaaa tgaaaggcca ggtaaatcat
     6721 gccattagtc attgttgcta tcggtgtaat cttgttgttg ctcctgatga tccgtttcaa
     6781 aatgaacggc ttcatcgctc tcgtcctcgt ggcgcttgct gttggattaa tgcaagggat
     6841 gccgctggat aaagttattg gctccatcaa agccggtgtc ggcgggacgc tcggtagcct
     6901 tgccctgatc atgggttttg gcgcgatgct gggcaaaatg ctggcagact gcggtggcgc
     6961 acaacgtatc gccaccaccc tgattgccaa atttggtaaa aaacacatcc agtgggcggt
     7021 ggtactgacc ggttttaccg ttggttttgc cctgttctat gaagtgggct ttgtgctgat
     7081 gctgccgctg gtgtttacca tcgcggcttc tgcgaatatt ccactgctgt atgttggtgt
     7141 accaatggca gctgcactgt ctgtgaccca cggcttcctg ccaccgcatc cgggtccgac
     7201 tgcgattgcc accattttca atgccgacat gggtaaaacc ctgctgtacg gtactattct
     7261 ggcaatcccg accgtgattc tcgccggtcc ggtttacgct cgcgtgttga aaggtatcga
     7321 taagccgatc cctgaaggtc tgtacagcgc gaaaaccttc agcgaagaag agatgccgag
     7381 ctttggcgtc agcgtctgga cctctctggt gccggtagtg ctgatggcga tgcgtgcgat
     7441 tgccgaaatg atcctgccga aaggtcacgc tttcctgccg gtagcggagt tcctcggtga
     7501 cccggtaatg gctacgctga ttgccgtgct gattgcgatg ttcacctttg gtctgaaccg
     7561 tggtcgttca atggatcaga ttaacgacac gctggtttct tccatcaaaa tcattgcgat
     7621 gatgctgttg atcatcggtg gtggcggtgc gttcaagcag gtgctggtag acagcggcgt
     7681 ggacaaatac attgcttcca tgatgcacga aaccaacatt tctccgctgc tgatggcctg
     7741 gtcgattgct gccgtactgc gtatcgcgct gggttccgca accgttgcgg caatcactgc
     7801 gggtggtatc gcggcaccgc tgattgcaac gacgggtgtt agcccggagc tgatggttat
     7861 tgcggtgggt tccggtagtg tgattttctc tcatgtgaac gatccgggct tctggctgtt
     7921 caaagagtac tttaacctga ctatcggcga gaccatcaaa tcctggtcga tgctggaaac
     7981 cattatctcg gtgtgcggtc tggtaggctg tctgctgctg aatatggtga tttgataaca
     8041 ctgactgccg gatgcatcgc atccggcatc aaccgcactc tactgcttct tcgctgcagc
     8101 tctgcgccgt ctgtccaaat ccttcagcaa cttattcacg ccatcatcgg caaacatcgt
     8161 ctcaagcgtt gcggaaagct tgcgtcgcca gtttttatac tggtaactgg tgccaggaat
     8221 attcaccggt tcggccatat ccagccagtc ttccggctgt agtcctaaca gagcactgtt
     8281 actgtcggca atgtagcgct gcaaaccacg attaagcgtc ggcgtcatcg acatcaacga
     8341 tgccttatgc ccggcacgtt ttggcagaca accatattta tgcagtgcat ccagcagccc
     8401 ttgcttcgcc agttcgcgat cctgatacag accgcgcagt accacttcat ccggatacag
     8461 ccccagggtt ttgcccaacg ttagatcccc gctctcccag taaccgcgca gcgtaggcag
     8521 gtcatgtgtc gccgcaaccg ccatcgactg ctccggatac gctttcggtg cacggaacgt
     8581 cttctcgtgg tcgttttcga aatagagcac tttgtaggag tacacaccgc tgctgcgcag
     8641 cttaccgaca atctctaccg gtacggtacc gagatcttca ccaatcacca tacagcgatg
     8701 acgtttactt tcgagtgcca gaatcgagag cagatcatcc accggatagt gaacatacgc
     8761 gccctgatct gccgtctcgc catacggtat ccaccacaaa cgcagcatcg acatcacatg
     8821 gtcaattcgt aatgcgccgc agttttgcat attggcacgc aacagctcga taaacggttc
     8881 ataggcacgc gcggtgatga tatgcgggtc cattggcggt aatccccagt tctgccccaa
     8941 cgggccgagg atatccggcg gcgcgccaac cgacgctttc aggcaatata gttcacggtc
     9001 acaccaggtt tccgccccac cttccgctac gccaaccgcc agatcacgat acaagccaat
     9061 cggcatttca tagccctggc ttatctccca gcaggcggca aactggctgt aagccagcca
     9121 ctgcaaccag agataaaaat cgacgtcatc acgatgttct tcgcagaact gacgcacttc
     9181 tggtgaatcc acgttctgat acatctctgg ccatgcaggc cagccccagc gcatttcgtc
     9241 ctctttcact tgctgggcat gtagcgcatc aaaggctgcc tgccagaaca ggctgtcgcc
     9301 ctgctctgca acaaactggc gaaacgcggt catctgctca tcatcacgtt gcgcgaaacc
     9361 tttccacgcc attcgtaatg ctgtcatttt tagggcggta accgtggagt aatcgaccca
     9421 gtcggcatcg cgcgcctgtt gcagcgtctg ttgcgtggtc ggcaactgcc accaggcctg
     9481 agcctcttcg ctaagatgga aatcttcaac ggcgttaacg tcgatataaa tcacattcag
     9541 ccaacggcga gaagacggac tgtatgggct ggcactctcc ggatttgccg gatagagcgc
     9601 atgaatcggg ttcaggccga tgaacgaccc gccacgtttt gccacatcca ccagcatcgc
     9661 tttgagatcg ccaaaatccc caatacccca gtttttttcc gatcgcagcg tataaagctg
     9721 aacgcaggca ccccacagct tttgtttatt cagcaacgcc tgcggttcgt aacagcgttt
     9781 cggggcgaca atcacccggc aatgcgcgcg ctggtcgtcc tgggtgagtg tcagcgtgtg
     9841 ataaccttcc ggcagcttcg tcggtagatt gaacgctttg ccccccgtta catggccttt
     9901 gtactgtgtt ccttcttcgg tggtcagcag ccagctatat tcgccgctgc cctccaccac
     9961 catcggcatt tttttgccgc tggtataaac catgacattc gggactggcg ttaccgccac
    10021 tttcgtggcg gtacgttgat gcatcgcgtc aagcaaacgc cgtttggttt cggcgctaat
    10081 cgactgcggt ttaccgtggg cattgatgta attggggcta atccccgccg ccagcgcggc
    10141 attatccaga cgtttgcttt ccatcgagct tccttagcgt tttgcctgcc agatacgagc
    10201 ctgataatcg cgaatagagc gatccgagct aaacataccg cagcgggcgg tattgaggat
    10261 cgccgcgcga gtccaggcct cctggtcgcg gtacagcaca tccacctgct tttgtgcctc
    10321 aacataggct gcgaaatccg ccatcaccag atacggatcg ccgccttgtt tgccgatact
    10381 gtgcagcatc tggtcgaagg catgcttatc gccgtcgctg tatttaccgc tttccagctc
    10441 tttcaatact gcgtccagca ccttatcttt cttccgccat ttcaccgggt cgtagccttt
    10501 ggccagaatt gccttcactt gttccacggt atgaccaaaa ataaagatat tttcttcacc
    10561 gactttctcg gcgatttcaa cgttcgcacc atccagcgta ccgacagtaa gcgcaccatt
    10621 gagcgccagt ttcatattgc cggtaccgga agcttcttta cctgcagtcg aaatttgttc
    10681 ggagatatcc gccgccggga tcagtttttc cgccgccgaa acgcaataat cctgcaggaa
    10741 caccaccttc aacttatcgc caaccagtgg atcgttgttg atcacgtcag ccactttgtt
    10801 gatcgcaaag ataatattct tcgccaggta gtagcccggt gccgctttcg cgccgaagag
    10861 gaagacgcgc ggtacgcgat cagcctgcgg gttttcacga atttctttgt acaacgccag
    10921 aatatgcagc agattcaggt gctggcgttt gtactcatgc agacgtttaa tctgaatatc
    10981 gaaaatcgcc tgtggattga tctcaatacc ggtacgaact ttcacaaact ccgccagacg
    11041 gactttattc gcctgcttga tctcgcgata ttgctgacgg aatttcgcat catcagcgaa
    11101 tttttccaga ttgatcagct gatcgagatc gttagcccac tctttttgca gtaatttatc
    11161 caacagagcc gccagtgcag ggttgcactg tttgatccag cgacgtgggg taataccgtt
    11221 ggtgacgtta tggaatttgt tcggccatag ctggtgatat tccgggaaca gatctttcac
    11281 caccagatcc gagtgcagcg ccgcaacacc gttcaccgcg aaaccgccaa ccacacacag
    11341 gttcgccata tgcacttgtt tatcgtgcac caccgccagt ttggcccaca ctttttcatc
    11401 gcccggccag gttttctcca ccagcgtttt aaagcgagta ttaatttcgt taataatctg
    11461 catatggcgc ggcagtaagc ctttcaccag tttcacatcc cagcgttcca gcgcttctgg
    11521 catcagggta tggttggtgt aagcgaaagt tttgctggta atggcccagg cgtcatccca
    11581 gctcatctgg tgctcatcga tcagcacgcg cagcagttct ggaatcgcga tagttgggtg
    11641 ggtatcgttc agctgaataa cttcataatc cgccagttcg tgcagtttac gccccgccag
    11701 atgatggcga cgcaaaatat ccgctaccga acaggcacac tggaagtact gctgcatcaa
    11761 gcgcagcttt ttaccggcag tatggttgtc gtttggatag agaactttgg tcagtttttc
    11821 cgcattgatg ccctgctgtt cggcacgcag gaaatcaccg tcgttaaatt tagtcagatc
    11881 aaacggatgc gcgtgcgtcg cctgccacag acgcagcggc tgcgccacgc cattacgata
    11941 gccgacaacg gggagatccc acgcttgacc ggtaatggta aactccggct cccagcgtcc
    12001 gtctttcgtc actttaccgc caatccctac ctgcacatcc agtgcttcgt tgtggcggaa
    12061 ccacgggtag ttactgcgat gccagtcatc cggcgcttca acctgtttgc catcgacaaa
    12121 agactggcgg aacaaaccat attgatagtt cagaccgtaa cccgtcgcag actgaccgat
    12181 agttgccatt gagtcgagga agcacgccgc cagacgtccc agaccaccgt tacccagcgc
    12241 cgggtcgatc tcttcttcca gcaggtccgt cagattgatg tcataagcct tcaacgaatc
    12301 ctgtacttcc tgataccagc cgagattcaa caggttgttg cccgtcaggc gaccaatcaa
    12361 aaactccatt gagatgtagt taacatgtcg ctgattcgcc accggcttgg cgaatggctg
    12421 agcacgcagc atttcggcca gtgcttcact cactgctagc caccactggc gaggagtcat
    12481 ttcagccgca gaatttaagc cataacgctg ccactgacgt gaaagcgctt cctgaaattg
    12541 cttatcgtta aaaataggtt gtgacatagg agttccactt ttcttagatt ttcaacacaa
    12601 cgttatcgct agtttgccag gctcgatgtt gaccttcctc atcctgcggg ggattaggca
    12661 gggaggagtt gcggggatga gcaaggaaat gtgatctcaa ccacttaaag ctagtgcaaa
    12721 ccacaggatt agcagcaaat caatgcaata cagcgcagaa aatctgtatc taagtgcaaa
    12781 aaatggccgt tgcgtatttt caaaaagcgg aaggtaactc tataaattaa gtaaaggagt
    12841 gaaacagttt cacaagtaaa atatccagtg tgctccatct cattcttaat agatttatta
    12901 agatcatctt tttagatggc actttcatca ggaatgaaga agaaaccctt gcttaaatga
    12961 atctgatgaa cataagggaa accagtattc acgctggatc agcgtcgttt taggtgagtt
    13021 gttaataaag atttggaatt gtgacacagt gcaaattcag acacataaaa aaacgtcatc
    13081 gcttgcatta gaaaggtttc tggccgacct tataaccatt aattacgaag cgcaaaaaaa
    13141 ataatatttc ctcattttcc acagtgaagt gattaactat gctgattccg tcaaaactaa
    13201 gtcgtccggt tcgactcgac cataccgtgg ttcgtgagcg cctgctggct aaactttccg
    13261 gcgcgaacaa cttccggctg gcgctgatca cgagtcctgc gggctacgga aagaccaccc
    13321 tcatttccca gtgggcggca ggcaaaaacg atatcggctg gtactcgctg gatgaaggtg
    13381 ataaccagca agagcgtttc gccagctatc tcattgccac cgtgcagcag gcaaccaacg
    13441 gtcactgtgc gatatgtgag acgatggcgc aaaaacggca atatgccagc ctgacgtcac
    13501 tcttcgccca gcttttcatt gagctggcgg aatggcatag cccactttat ctggtcatcg
    13561 atgactatca tctgatcact aatccagtga tccacgagtc aatgcgcttc tttattcgcc
    13621 atcaaccaga aaatctcacc ctggtggtgt tgtcacgcaa ccttccgcaa ctgggcattg
    13681 ccaatctgcg tgttcgtgac caactgctgg aaattggcag tcagcaactg gcatttaccc
    13741 atcaggaagc gaagcagttt tttgattgcc gtctgtcatc gccgattgaa gccgcagaaa
    13801 gcagtcggat ttgcgatgac gtttccggtt gggcgacggc actacagcta atcgccctct
    13861 ccgcccggca gaatacccac tcagcccata agtcggcacg ccgcctggcg ggaatcaatg
    13921 ccagccatct ttcggattat ctggtcgatg aggttttgga taacgtcgat ctcgcaacgc
    13981 gccattttct gttgaaaagc gccattttgc gctcaatgaa cgatgcgctc atcacccgtg
    14041 tgaccggcga agaaaacggg caaatgcgtc tcgaagagat tgagcgtcag gggctgtttt
    14101 tacagcggat ggatgatacc ggcgagtggt tctgctatca cccgctgttt ggcaacttcc
    14161 tgcgccagcg ctgccagtgg gaactggcgg cggagctgcc agtgggaact ggcggcggag
    14221 ctgccggaaa tccaccgtgc cgccgcagaa agctggatgg cccagggatt tcccagcgaa
    14281 gcgattcatc atgcgctggc ggcaggcgat gcgctgatgc tgcgcgatat tctgcttaat
    14341 cacgcctgga gtttgttcaa ccatagcgaa ctgtcgctgc tggaagagtc gcttaaggcc
    14401 ctgccgtggg acagtttgct ggaaaatccg cagttagtgt tattgcaggc gtggctaatg
    14461 caaagccaac atcgctatgg cgaagttaac accctgctag cccgtgctga acatgaaatc
    14521 aaggacatca gagaaggcac catgcacgca gaatttaacg ctctgcgcgc ccaggtggcg
    14581 attaacgatg gtaatccgga tgaagcggaa cggctggcaa aactggcact ggaagagctg
    14641 ccaccgggct ggttctatag ccgcattgtg gcaacctcgg tgctgggtga agtgctgcac
    14701 tgcaaaggcg aattgacccg ctcactggcg ctaatgcagc aaaccgaaca gatggcacgc
    14761 cagcacgacg tctggcacta cgccttatgg agtttaatcc agcaaagtga aattctgttt
    14821 gcccaagggt tcctgcaaac cgcgtgggaa acacaggaaa aagcgttcca gctgatcaac
    14881 gagcagcatc tggaacagct gccgatgcat gagtttctgg tgcgcatccg agcacaactg
    14941 ttatgggcct gggcacggct ggatgaagcc gaagcgtcgg cgcgtagcgg gattgaagtc
    15001 ttgtcgtctt atcagccaca gcaacagctt cagtgcctgg caatgttgat tcaatgctcg
    15061 ctggcccgtg gtgatttaga taacgcccgt agccagctga accgtctgga aaacctgctg
    15121 gggaatggca aatatcacag cgactggatc tctaacgcca acaaagtccg ggtgatttac
    15181 tggcaaatga ccggcgataa agccgccgct gccaactggt tgcgtcatac ggctaaaccg
    15241 gagtttgcga acaaccactt cctgcaaggt caatggcgca acattgcccg cgcacaaatc
    15301 ttgctgggcg agtttgaacc tgcggaaatt gttctcgaag aactcaatga aaatgcccgc
    15361 agtctgcggt tgatgagcga tctcaaccgt aacctgttgc tgcttaatca actgtactgg
    15421 caggccggac gtaaaagtga cgcccagcgc gtgttgctgg acgcattaaa actggcgaat
    15481 cgcaccggat ttatcagtca ttttgtcatc gaaggcgaag cgatggcgca acaactgcgc
    15541 cagctgattc aactcaatac gctgccggaa ctggaacagc atcgcgcgca gcgtattctg
    15601 cgagaaatca atcaacatca tcggcataaa ttcgcccatt tcgatgagaa tttcgttgaa
    15661 cgtctgctaa atcatccgga agtgccagaa ctgatccgca ccagcccgtt gacccaacgt
    15721 gaatggcagg tcctggggct gatctactcc ggttacagca atgagcaaat tgcgggcgag
    15781 ctggaagtgg cggcaaccac catcaagacg catatccgca atctgtacca aaaactcggc
    15841 gtcgcccacc gtcaggccgc ggtacaacac gcgcagaaac tgctgaagat gatgggatat
    15901 ggggtgtgag tttagccgga taatgcattg gatccggttt acatctttac atcattcaat
    15961 actcacccgc gttacgccat ctatttctat caaaccaaac cgcaccggca agaaacgctc
    16021 caccacggcg atattggtca gcaaatggca tgaaggatgg gcgaccgtaa attctcccgc
    16081 gcccgccagc gccatcggta gcaccagctg gtcggcgaga tattccccca ctgccgccgg
    16141 gcttgccagg tagcgtttca cctctttcac taactgcgcg gctaccacct cggcactgac
    16201 gcgcttttca ccgacgacga aaaagcgttc agtgatattt tcactttcga cttccagcga
    16261 gacggtatta cccggcccct ggtcgcgcgg caggttatga atattctgtt catgcaggga
    16321 aaaactcccc gccagtgtag cgatttcacg ctcagcaaca tggcgcggca caccagctaa
    16381 tagaacttct ccacgcatct gcacaatgtt cccgcgctcg ccaagttgca aggtgttaaa
    16441 cgatgccacg ggcgagactt ccgttgccac cacgccgcct ccggcaggat aaaaaccgtg
    16501 acgtaacagc gtggtttgct gatgaattcc tattttcgcc agcagcggct ccagcacccg
    16561 gcggataaaa tccgcaggcg gcgccgacgg gttatcggtg cctccgctca cttcaacacg
    16621 cgaaggtcca tcggcaaacc acagcgcggg cagcaccgtt tgcagcacca gcgtacagct
    16681 tccggcacta ccgatagcaa agcggtactc gccgccgcgc acggcgccag gccggaagac
    16741 cagacgctgc gaccccagct ccgcgccttc caccgttgcc ctacaaattt ccgcagccgc
    16801 ttttaccgcg gtcagatgct ggcgcaacag ccccggtttc gcccgcccgg cacgaatgct
    16861 ggtgatggta aatggctggc cggttatcat cgacaggctc agcgccgagc gcagaatctg
    16921 cccgccacct tcgccctgtg cgccatccag cgcaatcatc cttttcatca tttatccttt
    16981 tacgcacacc acctgacgca gggtatagat aacttccacc agatcgcttt gtgccgtcat
    17041 caccgcatca atatctttat acgccatcgg gatttcgtcg atcacttcgg catctttacg
    17101 gcattccaca tgcgcggtgg cacgaatttg atcttccacg ctgaacagtt ttttcgcttt
    17161 agttcggctc attacccgcc cggcaccgtg gctgcacgaa cagaacgact cttcatttcc
    17221 cagcccacgg acgataaagc tttttgctcc catcgaaccg ggaataattc catattgacc
    17281 agcacgcgca gacaccgcgc ctttacgcgt cacgtagatc tcttcaccaa agtgctgttc
    17341 tttttgcaca tagttgtggt gacagttgat ctcttccatc gccagcgttt gtggctgtct
    17401 gaccgttttc tgcgtaatgc tctgcaatgc cgttaccacg ttttccatca tcacatcgcg
    17461 gttaaggctg gcaaaaagct gcgcccaggc cacggctttc aggtaatcat caaagtattc
    17521 cgtaccttcc ataaagtacg ccagatcacg cgacggcaac gtctcaagcg tttcctgcat
    17581 ctctttttgt gccagatcga taaagtaagt cccgatggcg ttaccaattc cgcgtgaacc
    17641 ggagtgcagc ataatccaca cctggtccga ctcatcaagg cagattttaa taaagtggtt
    17701 accggttccc agcgttccca ggtgtttata gttattggta ttcaggaaac ggggatattt
    17761 ttgcgttaac cactgataac cggcttcaag ctcagcccat ttcgcatcga cgttgacagg
    17821 cgggttttcc catgcgcctt tatcacgttt acaacgcccg gtagtacgcc cgtgcggcac
    17881 ggccgtttca atcgcctgac gcagctctgc caggttttca ggcaggtctt ccgccgttaa
    17941 cgcggtacgc agcgcgttca ttccacagcc aatatccacg cccaccgccg ccggaataat
    18001 cgcccctttg gtcgggatca cgctaccaat ggtggaatct ttacccaggt gtacatcagg
    18061 cattaccgca atatgtttga aaataaacgg catcttcgcc gtattaataa gttgctgacg
    18121 cgcatcggct tctaccggca cgcctttggt ccacattttt accggggcat tttcagtggt
    18181 cagtaattcg taattcattt tgtttttctc ttttcgttgt ttgctgtcct gataattgca
    18241 accgtcgtgc cagaaaatta aataagtaaa ttaatttttt aattcattgt ttttaaaaag
    18301 attatatctt tatgtccgta accggagatt tcccgcaaag ccgatttacc gataatgaaa
    18361 tatcgtcttt tataaggata tctaagatgc gtaaaacagt agcttttggc tttgtcggaa
    18421 cggtgctgga ttatgccggg cgcggcagtc agcgctggtc aaaatggcat ccgacactct
    18481 gtttatgcca gcaagaatcg ttggtcatcg atcgactgga attattgcat gacgcccgct
    18541 cgtgctcgct atttgaaacg cttaaacgcg atatcgccag cgtttcgcca gaaacagaag
    18601 tggtgagcat tgagattgaa ctgcataacc cgtgggattt cgaagaggtt tacgcctgcc
    18661 tgcatgattt cgcccgtggt tacaagtttc agccagaaaa agaagactat ttaattcaca
    18721 tcaccaccgg cacccacgtc gcgcagattt gctggtttct gctggcggaa gcgcgttatc
    18781 tacccgcccg gttgatacaa acttcaccac cgcgcaaaaa agaacaaccg cgcggtgccg
    18841 gtgaagtgac gattatcgat ctcgatctaa gccgttataa cgccatcgcc agccgctttg
    18901 ccgaggaacg ccagcaaacg cttgattttc ttaagtccgg cattgccacg cgtaaccccc
    18961 acttcaaccg catgattgag cagattgaaa aagtggcgat taaatcccgc gcaccgatcc
    19021 tgcttaacgg cccaaccggt gcgggaaagt catttctggc gcgacgtata tttgaattaa
    19081 aacaggcgcg gcatcagttt agcggcgcgt ttgtggaagt gaactgcgcc accctgcgcg
    19141 gcgataccgc catgtcgacg ctgtttggtc atgtgaaagg cgcgtttacc ggggcgcggg
    19201 aatctcgtga aggtttatta cgcagcgcca acggcggaat gttgtttttt gatgagattg
    19261 gcgaactggg cgcagacgaa caggcaatgc tgctaaaagc cattgaagag aaaacctttt
    19321 acccgtttgg cagcgatcgc caggtgagca gtgattttca gcttatcgcc ggaacggtgc
    19381 gcgatttgcg ccagctggtt gccgaaggca aatttcgcga agatctgtac gcgcggatca
    19441 atctctggac cttcaccctg ccgggtctac gccagcgcca ggaagatatt gaaccgaacc
    19501 ttgattatga agtggagcgc cacgccacac tcactggcga cagcgtgcgt tttaacaccg
    19561 aagcgcggcg cgcctggttg gcttttgcga cctctcccca ggcaacatgg cgcggtaact
    19621 ttcgcgaact ttctgccagc gtcacgcgga tggccacctt tgccactagc ggacgcatca
    19681 ctctggacgt ggttgaagat gagataaacc gtctgcgcta taactggcag gagagccgcc
    19741 cctccgcgct tacggcgttg ctgggcgcag aggcagaaaa catcgatctc ttcgaccgta
    19801 tgcaactgga acacgttatc gctatctgcc gccaggcaaa gtcgctttct gcaaccggac
    19861 gacagctttt tgatgtttcg cgccagggaa aagccagcgt caatgacgcg gatcggctac
    19921 gcaaatacct ggcgcgtttt ggtctgacgt gggaagccgt gcaggatcag cacagctcca
    19981 gttgaatatg atggtccgtc agcacctgca ttacgcttac tggcggcggg gcgtcggtgt
    20041 agacggcatc taccatgctg atgctgccca tattgaccat cgcgttacgg ccaaatttcg
    20101 agtgatcgac aaccagcata acgtggcgcg agttctcaat aatggcgcgc ttggtgcgaa
    20161 cttcgtggta atcgaactcc agcagcgagc cgtcgctatc gatgccgctt atccccagaa
    20221 tgccgaaatc aaggcggaac tgggagataa aatcgagcgt cgcttcgcca atgatcccgc
    20281 catcgcggct gcgtaattcg ccaccagcga gaataatgcg gaaatcttct tttaccatca
    20341 acgtgttagc aacattgaga ttgttggtga caatacgcaa attgctgtga ttaagcagcg
    20401 cgtgcgccac cgcttccggc gtggtgccga tatcgataaa cagtgtcgag ccgttgggga
    20461 tttgctccgc cactttgcgg gcaatcctct ctttttcttc ggtctgggtg gctttgcgat
    20521 cgtgccacgg cgtgttaacc gaactggaag gcagcgccgc accgccatgg tgacgcagga
    20581 tcaggttttg ctccgccagc tcattgaggt cgcggcgaat agtctgcggg ctgacggaga
    20641 aatgctctac cagctcttca gtactgacat aaccctgctg tttaaccagt tcgataatac
    20701 cgttgtgacg ttgtgtttgt ttcatttata aatccctgga attattttcg ttttcgcgca
    20761 ttgagcgaat caacaaaagc catcgctaaa cccacggcta acccggcgat gtgtgctccg
    20821 ttcgccatcg acatcccaaa caaatcaaac catccggcga taatccagat cagcgcaaag
    20881 ataattaacc cacgttgcag gtaaatgcca ctttgcggat cgcgctcacc acgtagccag
    20941 acgtagccca tcagcgcata caccacgcca gaaagcccgc caaaccacgg cccgctgaat
    21001 ttttgctgca catagccgct taacagggca ctaatgagag taataacaat tagcttaccg
    21061 ctaccgaggc gtttttccac cgcaccgccg agataccacc accagagcag gttaaagagg
    21121 atatgcatca gcgagaagtg cattaacgcg tgggtgaagt aacgccagaa ctcaaatttc
    21181 agtgttggat cgaatggcca ggccagccat aacatcactt cctgatcgcc gagaatttgc
    21241 atggcgataa acaccaccac gcaggcgatc atcaccaccc aggttaccgg acctgcgcgt
    21301 tcacgcaagg cagcaaagaa aggataacgg cgataatgca gaccactgcc ggtatggccc
    21361 gcctgccagc tggccgccag ataacgcgga tctgccgggt tttcgagaaa acgcgccagc
    21421 tccgcccgca cgcgctcggc ctgggactca tccgccagcc agacatcgct ttggttatgt
    21481 tgttgaatcg taaggataac accctgcgtc gccatgtaat caacaaacgc ctgcgccacg
    21541 cgggggttag caaaagaggt aatcatcaac atcgttgctg tcgcttattc cacacaaaag
    21601 gggacagtat aaagcgttac gcgccgtacg ccacctctgc gggaaactga cgctgccagg
    21661 cttcaaaacc gccgtcaatg ctatagacca catcgtagcc ctgttgcagc agatactgcg
    21721 ccgcgccttt gctgctattg ccgtgataac acatcaccat caccggagtg tcaaagtcgt
    21781 tatcacgcat aaacgcgccc agcgtgtcgt tggttaaatg gaaagcctgc accgcatgtc
    21841 ccattgcgaa actctgtgga tcgcgaatat cgaccagcac cgcctctttt tcctgcaact
    21901 tctggtgcgc gtcggcaacg ttaatacatt cgaacagatc catgcgtctc tctttcttta
    21961 caaacaagtg ggcaaattta ccgcacagtt tacgtcgaag cggcagataa acgccataat
    22021 gttatacata tcactctaaa atgttttttc aatgttacct aaagcgcgat tctttgctaa
    22081 tatgttcgat aacgaacatt tatgagcttt aacgaaagtg aatgagggca gcatggaaac
    22141 caaagatctg attgtgatag gtggcggcat caatggtgct ggtatcgcgg cagacgccgc
    22201 tggacgcggt ttatccgtgc tgatgctgga ggcgcaggat ctcgcttgcg cgacctcttc
    22261 cgccagttca aaactcattc acggtggcct gcgctacctt gagcactatg aattccgcct
    22321 ggtcagcgag gcgctggctg aacgtgaagt gctgctgaaa atggctccgc atatcgcctt
    22381 cccaatgcgt tttcgcctgc cacatcgtcc gcatctgcgc ccggcgtgga tgattcggat
    22441 tggtctgttt atgtacgatc atctgggtaa acgcaccagc ctgccgggat caaccggttt
    22501 gcgttttggc gcaaactcag tgttaaaacc ggaaattaag cgcggattcg aatattctga
    22561 ctgttgggta gacgacgccc gtctggtact cgccaacgcc cagatggtgg tgcgtaaagg
    22621 cggcgaagtg cttactcgga ctcgcgccac ctctgctcgc cgcgaaaacg gcctgtggat
    22681 tgtggaagcg gaagatatcg ataccggcaa aaaatatagc tggcaagcgc gcggcctggt
    22741 taacgccacc ggcccgtggg tgaaacagtt cttcgacgag gggatgcatc agccttcgcc
    22801 ttatggcatt cgcctgatca aaggcagcca tattgtggtg ccgcgtgtgc atacccagaa
    22861 gcaggcctac attctgcaaa acgaagataa acgtattgtg ttcgtgatcc cgtggatgga
    22921 cgaattttcc atcatcggca ccaccgacgt agagtataaa ggcgatccga aagcggtgaa
    22981 gattgaagag agtgaaatca actatctgct gaaagtgtat aacgcgcact ttaaaaaaca
    23041 gcttagccgt gacgatatcg tctggaccta ctccggtgtg cgtccgctgt gtgatgatga
    23101 gtccgactcg ccgcaggcta ttacccgtga ttacaccctt gatatccatg atgaaaacgg
    23161 caaagcaccg ctgctgtcgg tattcggcgg taagctgacc acctaccgaa aactggcaga
    23221 acatgcgctg gaaaaactga cgccgtatta tcagggtatt ggcccggcgt ggacgaaaga
    23281 gagcgtgctg ccgggtggtg ccattgaagg cgaccgcgac gattacgcag ctcgtctgcg
    23341 ccgtcgctat ccattcctga ctgaatcgct ggcgcgtcat tacgcacgca cttacggcag
    23401 caacagcgag ctgctgctcg gcaatgcggg ggcgataagc gacctcgggg aggatttcgg
    23461 tcatgagttc tacgaagcgg aactgaaata cctggtggat cacgaatggg tccgccgcac
    23521 cgacgatgcc ctttggcgtc gcacaaaaca aggcatgtgg ctgaatgcgg atcaacaatc
    23581 tcgtgtgagt cagtggctgg tggaatatac gcagcagaag ttgtcgctgg cgtcgtaagg
    23641 atgatgcccg gttgaagtgc aaccgggcta tttggattcc ttagactatt cattgtaaat
    23701 aattgaataa cggcccctga gctaaaatcg ccaaacttaa gcacgttacc caatcgcccc
    23761 acttcttcct ctggtttcta ttttttctgc accaatggca ataatatccc aatgcaaaac
    23821 caagctgtgc actaatcggg ccgacaaaat cagtccaagg ctgcggccaa aaaggggaaa
    23881 aattcagggg aggccagaca aacgtcagaa agaaatttcg gatattttcc agcaacgctg
    23941 atttctctgg agaaatataa agaatgaaat tcgagagaat taaccagaaa tagaccggcg
    24001 aaaagaccag ccagcaatgc agtctgtttt ttaattcagg aggcgttctg cgggagaatt
    24061 gccaaccgat gccagccatt acac
//
LOCUS       DADLFA010000048        23492 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000048 DADLFA010000000
VERSION     DADLFA010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23492
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.048"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(297..611)
                     /gene="pspE"
                     /locus_tag="JQH65_001605"
     CDS             complement(297..611)
                     /gene="pspE"
                     /locus_tag="JQH65_001605"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HAY6139062.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(686..907)
                     /gene="pspD"
                     /locus_tag="JQH65_001606"
     CDS             complement(686..907)
                     /gene="pspD"
                     /locus_tag="JQH65_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HAY6139063.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(916..1275)
                     /gene="pspC"
                     /locus_tag="JQH65_001607"
     CDS             complement(916..1275)
                     /gene="pspC"
                     /locus_tag="JQH65_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HAY6139064.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(1275..1499)
                     /gene="pspB"
                     /locus_tag="JQH65_001608"
     CDS             complement(1275..1499)
                     /gene="pspB"
                     /locus_tag="JQH65_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HAY6139065.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(1553..2221)
                     /gene="pspA"
                     /locus_tag="JQH65_001609"
     CDS             complement(1553..2221)
                     /gene="pspA"
                     /locus_tag="JQH65_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HAY6139066.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKESGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            2388..3365
                     /gene="pspF"
                     /locus_tag="JQH65_001610"
     CDS             2388..3365
                     /gene="pspF"
                     /locus_tag="JQH65_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HAY6139067.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPATVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(3485..4750)
                     /gene="puuE"
                     /locus_tag="JQH65_001611"
     CDS             complement(3485..4750)
                     /gene="puuE"
                     /locus_tag="JQH65_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HAY6139068.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(4788..6020)
                     /locus_tag="JQH65_001612"
     CDS             complement(4788..6020)
                     /locus_tag="JQH65_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004984233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAY6139069.1"
                     /translation="MPFDTLNESITCDVCVVGGGYTGLSSALHLAEAGFDVVVLEASR
                     IGFGASGRNGGQLVNSYNRDIDVIEKSYGMDTAPMLGSMMFEGGEIIRERIKRYQIDC
                     DYRPGSLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAIRREVASDRYTGALLDH
                     SGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVVRTAKGQVTAKYVIVAG
                     NAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNYCVEDCNYLLDYYRLTA
                     DNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDYRWTGNFLLTLSRMPQF
                     GRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAFANLPHYPFPGGRTLRV
                     PFTAMGAAYYSLRDRLGV"
     gene            complement(6059..7546)
                     /gene="puuC"
                     /locus_tag="JQH65_001613"
     CDS             complement(6059..7546)
                     /gene="puuC"
                     /locus_tag="JQH65_001613"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HAY6139070.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLVGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FSDCSDLQKAASSTAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWMRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(7821..8378)
                     /gene="puuR"
                     /locus_tag="JQH65_001614"
     CDS             complement(7821..8378)
                     /gene="puuR"
                     /locus_tag="JQH65_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HAY6139071.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(8405..9168)
                     /gene="puuD"
                     /locus_tag="JQH65_001615"
                     /pseudo
     CDS             complement(8405..9168)
                     /gene="puuD"
                     /locus_tag="JQH65_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
     gene            9380..10798
                     /locus_tag="JQH65_001616"
     CDS             9380..10798
                     /locus_tag="JQH65_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HAY6139072.1"
                     /translation="METNIVEVENFVQQPEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDNRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKLLVRLMAEKHKMHSTFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            10947..11112
                     /locus_tag="JQH65_001617"
                     /pseudo
     CDS             10947..11112
                     /locus_tag="JQH65_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11102..12487
                     /gene="puuP"
                     /locus_tag="JQH65_001618"
     CDS             11102..12487
                     /gene="puuP"
                     /locus_tag="JQH65_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HAY6139073.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSINLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YDGDRTPVSET"
     gene            12621..12866
                     /locus_tag="JQH65_001619"
     CDS             12621..12866
                     /locus_tag="JQH65_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HAY6139074.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            13179..14822
                     /gene="sapA"
                     /locus_tag="JQH65_001620"
     CDS             13179..14822
                     /gene="sapA"
                     /locus_tag="JQH65_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HAY6139075.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            14819..15784
                     /gene="sapB"
                     /locus_tag="JQH65_001621"
     CDS             14819..15784
                     /gene="sapB"
                     /locus_tag="JQH65_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HAY6139076.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            15771..16661
                     /gene="sapC"
                     /locus_tag="JQH65_001622"
     CDS             15771..16661
                     /gene="sapC"
                     /locus_tag="JQH65_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HAY6139077.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            16661..17653
                     /gene="sapD"
                     /locus_tag="JQH65_001623"
     CDS             16661..17653
                     /gene="sapD"
                     /locus_tag="JQH65_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HAY6139078.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVIADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            17655..18461
                     /gene="sapF"
                     /locus_tag="JQH65_001624"
     CDS             17655..18461
                     /gene="sapF"
                     /locus_tag="JQH65_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HAY6139079.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGKVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            18529..18882
                     /locus_tag="JQH65_001625"
     CDS             18529..18882
                     /locus_tag="JQH65_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HAY6139080.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            19250..20038
                     /gene="fabI"
                     /locus_tag="JQH65_001626"
     CDS             19250..20038
                     /gene="fabI"
                     /locus_tag="JQH65_001626"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAY6139081.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            20183..21310
                     /locus_tag="JQH65_001627"
     CDS             20183..21310
                     /locus_tag="JQH65_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HAY6139082.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTARDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEYTKSSTAELCQLAETLSLHPISLSLLERLLNSTRVNTQPDNKLAALLCARIN
                     GSPACFAACMDSSNEYKKISPLLRKGENEINQWADRHSVEHATVQAIQWLTRAPDRFS
                     TAQFSPLLEHEQSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            21378..23312
                     /gene="rnb"
                     /locus_tag="JQH65_001628"
     CDS             21378..23312
                     /gene="rnb"
                     /locus_tag="JQH65_001628"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HAY6139083.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGAIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAMRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(23390..23492)
                     /locus_tag="JQH65_001629"
                     /pseudo
     CDS             complement(23390..23492)
                     /locus_tag="JQH65_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         5307 a         6208 c         6117 g         5860 t
ORIGIN      
        1 gacgcgttac aagtttctgt aaatgagttg cggtaaatgt tccaccgtag atttcgtcca
       61 ggtaatcagt aattttctgt ttcatgttgc ctttcagtgg tagcgatccc atgaaacgag
      121 agactactcc gcagaaaata aactgtcaat tgagcacaag ggttttagtc acgcaggaaa
      181 tcatttttta ccagggaaaa agcgtatttg tgagctgacg caaataatct atggggatgt
      241 aaataaggtg tgacagaaaa aaaacggcgc ataagcgccg ctcatggtga attcttttaa
      301 cctttgacct tcggcattgc gatgtctttc aagccaccgg cgttctcaac gtgggtatat
      361 cccatctcgc taaggatctc ttttgcttgc cctgactggc gtccggcatt gcaatacact
      421 ttcacggtgt cgtttttatc cggaacggcg gtggcaatgc gctctttcac ttccttcagg
      481 ggaatattga tggccccctg aacgtgctct tgctgatact gctctggaac acgaacatcg
      541 atccagtgtt cagcggcgaa aacgggcagt gaaaacacca gtgccagagc aagtaagcct
      601 tttttaaaca tgtaagcgtc cttctatgga tgaaaattgc catccattat acgcctgaga
      661 tttaacaatt tttgcgaaac tcccctcacc ttttataacg ctgtgccagt ttattagcag
      721 cccgacttaa cagcggttcc agcgccacag ccagcaacat tttcagcggt cggcgagcaa
      781 ctgattttat cgcccagccc gctacacccg ccgggccata gcgcagtgcg gtaagaagta
      841 ccagcttgcc tgctaatttg aaaccaggct ttaccttttg cccggcctgt tgccagcgag
      901 tattcataac tttcctcaca gttgacggaa acggctacgt aacgtgaaag tatcggaagt
      961 gacataacgt tccatctcgc gtaaacgcgt ttcacttgcc gccagttcac ggtcgacttc
     1021 atccagcaat tcgctgctgg agggtagctg ctcaccaaag gccatgttgt ccggcattgg
     1081 atcaagcgca aatgacaaaa tgatgtaagc aaccagggta aacagcgcca gaccgaagaa
     1141 aatcgacagc accaccagga tacgcaccag ttttaccggt acatcaaaat agttggcaat
     1201 cccggcgcag acgccgcgga ccatgccctg ctgtggaata cgccataatt ttttattgag
     1261 attaatgccc gccattagcg atccctccag ttcggatgtt ctgcatcaag aatagattcc
     1321 agcgcctgaa tacgttcgcg catccgtttt gcttcatcag ccagttgcgc taatcgctgc
     1381 tgctcacttt gcgacaattc actgcgacca gaacgattgc tgtaatgcag ccataaccag
     1441 atcggtaaaa caaacagcac aaaaatggtt aacggaatag ccagaaatag cgcgctcata
     1501 agtactcctt agacgatgag cggcggcgcg ttcggacgcc gcctagatgt tattattgat
     1561 tgtcttgctt cattttggct tttaattgtg ccagttgttc gctgattgca tcatcggctt
     1621 tcagttcggc aaactgatcg tccagcgatt tttgtttacc gaagctgtgg ctttctgctt
     1681 ccgcttccat ctggtcaata cgacgttcga aagattcgaa acgagccatt gcttcatcca
     1741 gtttgccact gtccagctga cgacgcacat cgcgcgacga gtttgccgcc tgatggcgta
     1801 acatcaatgc ctgctggcga gcgcgtgttt cgctcaattt gttttccagc tcaccgctct
     1861 ctttcttcat gcgtgccagc gtatcgtcca ccagcgtcac ttcatgttcc agactcttaa
     1921 tcagatcggt cagtttctgt ttttcaatta acgccgcacg tgccaaatcc tctctctctt
     1981 tcagcagcgc cagttcggct ttttcctgcc attcaacttc acgcgccgac gcttgttcaa
     2041 tacggcgagt cagctgtttc ttttctgcca acgcacgcgc cgaagtagaa cgtacttcaa
     2101 ccagtgtatc ttccatctcc tggatcatca gacgcaccag tttctgtgga tcttccgctt
     2161 tctctaacag agcgttgatg ttggcattca cgatgtcggc aaagcgagaa aaaataccca
     2221 taatgttgtc ctcttgattt ctgcgttcag gattgtcctg ctgaactgtt aatacaattt
     2281 gcgtgccaat tttttatctt tttgatttat aaagatctga ttgaagaatc atcagcaaca
     2341 tgccaggatg aattagcgaa ttacactaac aagtggcgaa tttcatcatg gcagaataca
     2401 aagataattt acttggtgag gcgaacagct ttctcgaagt gctggaacag gtttcgcatc
     2461 tcgcaccgct ggacaaaccg gtgctcatca tcggcgaacg cggcaccggt aaagagctga
     2521 ttgccagccg cctgcattat ctctcctccc gttggcaagg gccgtttatt tcccttaact
     2581 gcgcggcgtt aaatgaaaat ctgctggatt ccgaactgtt tggtcacgaa gcgggggcgt
     2641 ttaccggtgc gcaaaaacgt catccaggga gatttgaacg tgccgacggc ggtacgctat
     2701 ttcttgatga actcgctacg gcaccgatga tggtgcagga gaaattattg cgcgtgattg
     2761 agtacggtga actggagcgc gttggcggca gccagccatt gcaggtgaat gtgcggttgg
     2821 tatgcgcgac gaatgccgat ctcccggcga cggtcaatga aggcactttt cgcgctgacc
     2881 tgctcgaccg actggctttt gatgttgtac aactgccacc actgcgcgag cgcgaaagcg
     2941 acataatgtt gatggcagaa cactttgcca tccagatgtg tcgggaaatc aagctgcctc
     3001 tgttcccggg gtttacggag cgcgccagag aaacattgct gaattatcgt tggccgggaa
     3061 atattcgtga attgaaaaac gtggtggaac gttcagtgta tcgccacggc accagcgatt
     3121 atccgcttga tgacatcatt attgatccct ttaaacggcg tccgcctgaa gacgctatcg
     3181 ccgtttcaga aaccacctcg cttccaacac tgccgctgga tttacgtgag tttcagatgc
     3241 agcaggaaaa agagttgctg caactcagtt tgcaacaagg gaaatataac cagaagcgcg
     3301 cggctgaatt actggggtta acttatcatc agttccgcgc gttgttgaaa aagcaccaga
     3361 tttagcgaag ttgagcaata caacttgccg gatgcggcgt gaatgcttta ttcggcatac
     3421 ataatcaata agtcgtttgc aggcctgata agcgcagcgc atcagacatt attgcgttgg
     3481 gctattaatc gctcagcgca tcctgcaaaa ttttcattgc cgcatcgaat tgcgcatccg
     3541 gaatggtcag cggatacagg aagcgaatca cgttgccgta tgcgccacag gtcagcagga
     3601 gcagcccctg cgccagcgcg cgttgctgga ttttctgtgc aatcgccgct gacggctcgc
     3661 ccgtttgcgg atcgttaaac tctgccgcaa tcatcgaccc caggccgcgt accgcagcaa
     3721 tggccggaac gctttctttg gcatcaatca acgtgttttt gagacgctgg cccagttgat
     3781 tcgcgcgttc gcagagtgat tctttgtcga taatattgag caccgcgtgc gcggcagcca
     3841 ccgccagcgg gttcccggcg taggtgccac ccaacccgcc cggcgcgggc gcgtccataa
     3901 tattcgcgtt accgaccacg cccgaaagcg gcatcccgcc cgcgaggctt ttcgccatcg
     3961 tcattaagtc cggcttatcg gcgtaatgat ccatggcaaa cagtttaccg gtacgcgcaa
     4021 agccgctttg cacttcatcg gcaatcatca caataccgtg ctcgtcgcac aggcggcgaa
     4081 tggcggcaac cagctctttt ggcgcaacgt tgaaaccgcc ctcgccctgc accggttcga
     4141 aaataatggc cgccacctgc ttcgcttcaa tgtccgattt aaacaagcgt tcgatggcgt
     4201 cgagggagtc ctgtgttgaa atgccgtgta aatctgacgg ataaggtacg tgatacaccg
     4261 aaccagggaa cgggccgaag ccgattttgt acggcgcaac ttttccggtc agcgccatgg
     4321 tcatatatgt acgaccgtgg aagccgccgc taaacgcaat cacgccaggt cgtccggtat
     4381 gggcgcgagc aattttcacc gcgttttcca ccgcttccgc gccggtagtg aagaatgcgg
     4441 ttttagcctg cccgctcact ggggcaaggg cgttaatttt ctccgccagc gtgacgtagc
     4501 tttcgtacgg cacaatctga tacgccgtgt gggtaaactg ttgcaattgc tgctccaccg
     4561 ccgcgaccag atcagggtgg cgatgcccgg tattcagcac cgcaatgcct gcggcgaaat
     4621 cgatgtattc gttgccctct acgtccttca gcgtagcgtt ttctgccgac tgggcgaaga
     4681 agttacacat cacgccaacc ccgcgtgggg tagcagaaag acgacgctga tggaattcat
     4741 tgttgctcat gtttcacacc ttttgactct tcactgttaa tcggaaatta aacgcccaga
     4801 cgatcgcgca ggctgtaata agccgcgccc atcgcggtaa atggcacgcg cagcgtgcgc
     4861 ccgccgggga acgggtaatg cggcagattg gcgaaggcat cgaaacgctc ggcgtcaccg
     4921 cgcagcagtt cagcaatcaa acgcccggcg aggtgggtac aggtgacgcc gtggccgctg
     4981 tagccctgca tgtaatagat gttggtatcg aggcgaccga actgcggcat ccgtgaaagg
     5041 gtcagcagga agttgcccgt ccagcgatag tcgattttga cgcccttcag ctgcgggaag
     5101 gttttcagca gtttcggcac aacaagacgc tcaacgtcat ccgggtcgcg cgcgccgtag
     5161 accacgccgc cgccgtacag caaacggttg tcggcggtaa gacggtagta atcaagcaga
     5221 taattacagt cttccacaca gtagtttttc gggatcagcg aacgggctaa atcttccgac
     5281 agccgttcgg tggtgatcac ctgcgtgccg cacggcatgc tgcgtttcgc cagttccggc
     5341 tctactttat cgcccagata cgcattcccg gcgacaatca catacttcgc cgtcacctga
     5401 cctttggcag tgcgcacaac ggctggcgtg gtgtgctgga tctgcgtcac ggcagaaagt
     5461 tcatacacgc gcccgccgtt gaggcggatg gcgtccgctt cgccaatggc gaggtttagt
     5521 gggtgaatat gcccaccgct gtgatccagc agcgcaccgg tatagcgatc gctggctact
     5581 tcacggcgaa tggcgttggc gtccagcaat tccagctgtt tattgccgta gcgttcccag
     5641 ttctctttct gctcttcaag tgtggcgagc tgtttatcat tcatcgccac aaacaggctg
     5701 ccggggcggt agtcgcagtc aatctgataa cgtttgatac gttcgcggat gatctcacca
     5761 ccttcgaaca tcatgctgcc gagcataggg gcggtgtcca tgccgtagct tttttcgatc
     5821 acgtcgatgt cgcggttgta ggagttcaca agctgcccgc cattgcgccc gcttgcgcca
     5881 aagccgatgc gtgaggcttc gagaaccact acgtcaaagc ccgcttccgc cagatgcagc
     5941 gcggaggaga gcccggtata gccgccgcca accacgcaaa cgtcgcaggt gattgactca
     6001 ttcagggtgt cgaatggcac gtatttatgc gtaataactg ctggtatgtt cggtcatttc
     6061 aggcctccag gcttatccag atggttttca gttcggtgaa tttttcaagg gcatgcaggg
     6121 atttgtcgcg accgttgccg ctctgcttat agccgccaaa cggcacggtc atatcgccgt
     6181 cgttgtagtt attgacgaag acggaaccgg ctttcaggcg gcggctcata cggtgcgcgc
     6241 gggagaggtc gcgcatccat accgccgcgc caaggccgta ctggctgtcg ttggcaagct
     6301 gtagcgcctg ttcttctgat gtgaaacgcg tgaccaccag caccggaccg aaaatctctt
     6361 cgcggcttaa ggacgcattc gggtctacat ccacaaagat ggtcgggccg atggcggcag
     6421 ccagcccggc gttacggcca tccagcagca gttgcccttt gccttcgccc tcacgaataa
     6481 agctatggac cgagtcggcg tgggcgcagt cgattaaagt gcccatggtg gttgcggggt
     6541 caagcggatg gcccggctgc cagttttgcg cctgctgttt taacagggct aagaattcat
     6601 cggcgatgct ctcttccagc aacaggcgtg ttccggcgat gcacacctga ccctggttgt
     6661 agaagatgcc tgctgcggtg gagctcgccg ctttttgcag atccgaacaa tcggagaaaa
     6721 cgatgttggc gcttttgccg cccgcttcca gccagacgcg tttcatgttg ctgtcgcccg
     6781 catctttcag cagctgtttc ccggtacggg ttgaaccggt aaaggcaatg gcgtcgatat
     6841 cgttatgacg cgacagcgcc tgccccgctt catgaccaaa acccgtcacc acgttcaaca
     6901 caccatccgg caagcctgct tctttcgcca gccccacgag acgaatcgca ctgagcggtg
     6961 atttttcaga cggttttaga atcacgctgt ttcccgccgc cagcgccggg ccgagtttcc
     7021 agcaagtcag caacagcggg aagttccacg gcacgatggc ggcaatcacg ccgaccggtt
     7081 cacgcacgat catcgccagc tcatggctac tggtggtcgc cacttcgcca tacactttgt
     7141 cgatcgcttc ggcgtaccag cgaatggcgc gcgccgcgcc gggaatatca tcacgcagac
     7201 tgtgacgaat cggtttgccg gtgtcgagag tttccagcag tgccagctct tcggcgtggg
     7261 cttccattaa atcggcgagt ttattcagta ccgctttacg tttagccgga gaagagagtg
     7321 accagtcgcc gcgttcaaat acgccgcgtg ctgcgctcac cgcacggtcg atatcgacgc
     7381 tcttgccgcg ggcaattttc gccagcggtg cctgggtgac tggatcaacg gtttcaaagg
     7441 tttcattttc cgccgcagca gtatattcac cgttaataaa taagcggttt tcaatggcga
     7501 gacttaacgc tttatcctgc cagtaagcca gatgatgaaa attcattatg actcctgttt
     7561 cacgtctatc agatatatgc agtttgtgaa cgtcagggcg cagagatccc cgggccgtat
     7621 gcccgtttat ttctggcgac attctttata tacacttcat ccttcaagcc gcctctgcct
     7681 tggctgcgtt cgctcccccg aatcacttac ctgagtaagc tcatcgggat tcgctcactt
     7741 gccgccttga tgcaacttga atgattttgt gtatagattg ccgggcgcat tgcgcttacc
     7801 cggcttacag aacaaaaaga ttaaaacgtg gtgggcgtat gggcgctgat aattcggcaa
     7861 atacctgccg acgtattact gaaactgtgc gggatgccgg tattaatggc ataactttga
     7921 cccgcgacga ggtggtaatc ctgaccatta atcgtcagaa caatttcacc ttccagtact
     7981 gtgcctattt cctcaccctg atgcttaatt ctttccccag tggttgtgcc cggctggtac
     8041 gtttcaaaga tcatcgccag cgtgcgattc gggttaccgt tatgaaccag cttcattgac
     8101 acaccctgac tgcccatctc aattaagtcg tcctgattaa tgacgacctg cggctcatca
     8161 ggtttttccg gctcggaaaa gaattccgag agtgacagac catacacctt cagcagcttt
     8221 tgcagcgtac tgatggcagg gctgacttta tcttgttcta tcgtactgat agcactgtga
     8281 gtcagcccgg agagttcggc ggcacgacgt tgtgaaagcc cctgctgctg gcggatttcc
     8341 gacaagcgtt ttcctggcgc cagtccctca tcactcatat ttgcatttcc ttaaactgta
     8401 gtggtcagag tcgctgtttt tcagcgatat ggtgctgaca agcggtgata aagccctcga
     8461 acaatatacg cgaaagagcg tactcgctac tgttccattc cgggtgccac tgtacgccca
     8521 gcgcaaaagg atgattgatg acgctaaccg cctcaactaa gccatccggc gagcgagctt
     8581 caacacgcaa ccgtgggcta acgaccttcg ccccttgtcc atgtagagag tttacccaaa
     8641 agttgctaca ttcaggtaac aacgcagaca gtaatccccc ctcttcaacc tgaacttcat
     8701 gcgacggtgc atattgctgt tccaccggca gttcggatct tcccgatgtt ccagcaattc
     8761 aggctgttcg cacagcttgc gatgcaacga accaccggtt gccaccacca gttcttgtaa
     8821 accccggcag atggcgaaaa tggggatgcg cctttcgagt gcggcattaa ttatcgccat
     8881 gctcagaaga tcacgcccgg gatcggcgtc aggctcatcg ccgttttcac catataggtg
     8941 cggctgcaca ttgctgggac taccaggaag ataaatgcca tcgagtttcg gcaaaagttg
     9001 ttcaagtaac gacggttccg ccagcgcatg tggtagcgca attggcaagc cgcctgcgtg
     9061 gatgatggca ttcaggtact tttcttgcag agtctgggtc gcatgaccct taagcctgtt
     9121 cctgcacatt acgacaccga taaccggatt gttcattata tttttcatgc tcaatctcac
     9181 aaagtggact aaattatcgc cattactgcg ctttaaatga cctttatgtt caatattttt
     9241 tcaatctagc agtgggtttt catttttgca aactaaattt aacatttggc aaacatttag
     9301 tttgcataca gattcgaatg gtggtcatta tattttacgc tttgataacg agcggaaaac
     9361 aaaccaaagg cgaagaatca tggaaaccaa tatcgttgaa gtagagaact ttgttcagca
     9421 gccagaagag aggcggggta gcgcctttac gcaggaagtg aaacgctacc tggagcgcta
     9481 cccgaatacg caatatgttg atgttttact tactgatttg aatggttgct tccgtggaaa
     9541 acgtatccct gtttcaagcc tgaagaagtt ggagaaaggg tgttatttcc cggcctcggt
     9601 gtttgcaatg gatattctgg gcaacgtagt agaagaagcg ggtctgggtc aggaaatggg
     9661 cgagccggat cgtacttgtg ttcctgttct cggttcctta accccttctg ccgccgatcc
     9721 agagtttatc ggtcagatgc tcctgaccat ggtcgatgaa gatggcgctc cctttgacgt
     9781 tgagccgcgg aacgttctta accgcctgtg gcagcagcta cgccagcgcg gattgttccc
     9841 ggttgtagcg gtagagctgg agttctattt actggatcgt cagcgcgacg ctgaagggta
     9901 tctgcaaccg ccctgcgcgc ccggcaccga taaccgcaat acacaaagcc aggtttactc
     9961 cgttgataac ctcaaccact tcgccgacgt gctcaatgat attgatgaac tggcgcagtt
    10021 acagttaatt cccgccgacg gtgcggtcgc cgaagcctcg ccgggtcagt ttgaaatcaa
    10081 cctttaccat accgataacg tgctggaagc ctgcgatgat gcgctggcgc taaaactcct
    10141 ggtgcgtctg atggcagaaa agcataagat gcactccact tttatggcga agccgtatga
    10201 agagcacgcg ggcagcggga tgcatatcca tatcagtatg caaaataatc gtggcgagaa
    10261 tgtgctttct gacacagaag gcgaagattc gccgctgctg aaaaagatgc tcgccgggat
    10321 gattgacctg atgccgtcgt cgatggcgtt gctggcacca aacgtgaact cgtatcgccg
    10381 cttccagccg ggaatgtatg tgccgacgca ggcgtcgtgg ggccataaca accgcaccgt
    10441 cgccctgcgt attccgtgcg gcgaccgtca taatcaccgc gtggaatatc gcgtggcggg
    10501 tgccgatgcc aacccatatc tggtgatggc agcgattttt gccggtattt tgcatggcct
    10561 tgataacgag ctgccgttgc aggaagaagt cgaaggcaac gggctggaac aggaaggctt
    10621 acccttcccg attcgccaga gcgatgccct gggtgagttt atcgagaatg atcacctgcg
    10681 ccgctattta ggcgaacggt tttgccatgt gtatcacgcc tgtaaaaatg atgagctgtt
    10741 gcagtttgag cgcctcatca cagaaacaga aattgagtgg atgttgaaaa acgcatagta
    10801 ccccttgcgg ggttgtttca tcgacctgag cggagccagg cctgacattt gctggtaatc
    10861 cagacatttc ccggatgtcg cgttatggag cgcaggcggc aacgggcttc cgttaacgat
    10921 taagactgtg acgaggaaat gagatgatgc cgatgattaa atccccgcat ggcgagggag
    10981 gctgcgtatg cgcccctcct gctactgact ggactccgtc ccctttgctt cccttgctaa
    11041 acaggtttga tttcaggtcg acacgaccgc aaaccttatt acgcaggggg aggcagcaat
    11101 tatggctatt aattcaccac tgaatattgc tgcgcaaccc ggcaaaaccc gtctgcgaaa
    11161 atcactgaaa ttgtggcagg tggtgatgat gggtctggcc tatctcacgc cgatgaccgt
    11221 atttgatact tttggtattg tctccggcat tagcgacggt cacgttccgg cgtcctattt
    11281 gctggcgctg gcgggcgtgc tgtttaccgc tatcagctac ggcaaactgg ttcgccagtt
    11341 tccggaggcc ggttcggcct atacctacgc gcaaaagtcg attaacccgc acgtcggatt
    11401 tatggtcggc tggtcatcgc tgctggatta tctctttttg ccgatgatca acgtcttgtt
    11461 ggcgaaaatc tatctctccg ccctcttccc ggaagtgccg ccgtgggtgt gggtagtaac
    11521 cttcgtcgcc attttaaccg ccgcgaatct gaagagcatc aacctggtcg ctaacttcaa
    11581 taccctgttt gtactggtgc aaatctccat catggtggtg tttatcttcc tggtagttca
    11641 gggactgcat aaaggagaag gcgttggcac cgtctggtca cttcagccgt ttatcagcga
    11701 gaacgcgcac ctgatcccga ttattaccgg ggcgacgatt gtctgtttct ccttcctcgg
    11761 tttcgatgcg gtgaccacgc tttcggaaga gacgccggac gccgcacgcg tgatcccgaa
    11821 agccatcttc ctgacggcgg tctacggtgg cgttatcttt atcgcggcgt cgttctttat
    11881 gcagctgttc tttcccgata tcagccgctt taaagatccg gatgccgcac tgcctgaaat
    11941 tgcgctgtac gtcggcggca agctcttcca gtcgattttc ctctgcacca cgtttgtgaa
    12001 cacgttagcc tctggcctgg cttcacatgc cagcgtgtcg cgtctgctgt atgtgatggg
    12061 gcgtgacaat gtgtttccgg agcgcgtgtt tggctatgtg cacccaaaat ggcggactcc
    12121 ggcactcaac gtcattatgg tcgggattgt cgcgttgtcg gcgctgttct tcgatttagt
    12181 taccgccaca gcgttaatta acttcggtgc tctggtggcg tttaccttcg tgaatctgtc
    12241 ggtgtttaat catttctggc ggcgtaaagg aatgaataaa agctggaagg atcacttcca
    12301 ctatttgctg atgccgctgg ttggcgcgct gacggtgggt gtgctgtggg ttaacctcga
    12361 gtcaacgtca ctgacactcg gtctggtatg ggcttcgtta ggcggcgcat atttatggta
    12421 tctgatccgc cgctatcgca aagtgccgtt gtatgatggt gacagaacgc cggtgagtga
    12481 aacgtaatag tcggatgcgc ggcgcattat cctgccggag ggtaatgcac cgcgccatta
    12541 actataaaaa gatgtatgac ccggatcacg agtgctatca tccgccctta tgcttagcgc
    12601 attaacccca ggggcaaata atgaaccacg atatcccgct aaaatatttt gatattgccg
    12661 atgagtacgc gaccgaatgc gcagagcctg tcgcagacgc tgaacgcaca ccgctggccc
    12721 actacttcca gttactgctc acccgtttga tgaacaatga agagatcagc gaagaagccc
    12781 aacatgaaat ggctgctgaa gcggggatta atcccgtacg cattgatgag atcgcagagt
    12841 ttttgaatca atggggtaat gagtaattat ttaattgcag acgatcggtc agtatcgttg
    12901 tccggataaa gcgttcacgc cgcatccggc taacaatgac acgcactgca gcgtgcttta
    12961 atgggtttct cacgatcgta ccgctctgct cctcgctaag ttgttctgtg agttatagtt
    13021 atagaggaag cacgggtacg aatgttgaac agatggcgac gccacctgtt caacgaccaa
    13081 ccacacggcc ctcattacga cccctgaaca caaacggtaa tggcaaatgt cattaaatat
    13141 tagtaagata gcctctttac ataaaatccc ttaatattat gcgccaggta ttatcgtctc
    13201 ttttggtgat tgctggactt gtgagtggtc aggcaatcgc cgcgcctgaa tcccccccgc
    13261 atgctgatat ccgcgacagc ggttttgtct attgcgtcag cgggcaagtc aacaccttta
    13321 acccatccaa agcgagcagt gggttaattg tcgataccct tgccgcccag ttttatgatc
    13381 gactgctgga tgtcgatccc tatacctatc gcctgatgcc ggaacttgcc gaaagctggg
    13441 aagtactcga caacggcgcg acctatcgct tccacctgcg tcgcgatgtt ccgtttcaaa
    13501 aaaccgactg gtttactccc actcgtaaaa tgaatgccga cgatgtggtg tttaccttcc
    13561 agcgaatttt tgaccgcaac aacccgtggc ataacgtcaa cggcagcaac ttcccctact
    13621 tcgacagcct gcaatttgcc gataacgtga aaagcgtccg caaactggat aatcataccg
    13681 ttgagttccg actcgcccag ccggatgctt cttttttgtg gcacctcgca acccattatg
    13741 cttcggtcat gtcggcagaa tatgcccgga agttagagaa agaagatcgc caggagcaac
    13801 tcgaccgtca accggtcggc accggtccgt atcagttgtc ggaataccgc gccgggcaat
    13861 ttattcgcct acaacgtcat gatgacttct ggcgcggtaa accgttaatg ccgcaggtag
    13921 tggtggattt aggctccggc ggcaccggac gtctgtcgaa actcctgacc ggggaatgcg
    13981 acgttctggc ctggcctgct gccagccagc tatccatttt gcgtgacgac ccgcgcttgc
    14041 gtttaacgct gcgtcctggg atgaacgtcg cctatctggc atttaacacc gccaaaccgc
    14101 cgctaaataa tcccgctgtc cgccatgcac tggcactggc gattaataac cagcgcctga
    14161 tgcaatccat ctattatggt acggctgaaa cggcggcctc tattttaccg cgcgcctcgt
    14221 gggcctatga caacgaggct aaaattactg aatacaatcc ggcgaaatcg cgcgaacagt
    14281 tgaaggcgtt ggggctggaa aacttaacgc tgaaactgtg ggtgcccaca cgttcgcagg
    14341 cgtggaaccc cagtccactg aaaactgccg aactgattca ggcggatatg gcgcaggttg
    14401 gcgtaaaagt ggtgattgtg ccggtagaag gtcgctttca ggaggcgcgg ttgatggata
    14461 tgagccatga tctgacgtta tccggttggg cgacggacag taacgacccg gacagtttct
    14521 tccgtccttt actgagctgc gcggcaattc attcacagac caacctcgcc cactggtgcg
    14581 atccgaaatt cgacagcgtg ttgcgtaagg cgctctcctc gcagcagctg gcggcgcgta
    14641 ttgaagccta tgacgaagcg cagagtattc tggcgcagga attgcccatt ttgccgctgg
    14701 cgtcgtcatt gcgtttgcag gcctatcggt acgatatcaa aggtctggta cttagcccgt
    14761 ttggtaacgc ctcctttgct ggggtgtatc gcgagaaaca ggatgaggtg aaaaaaccat
    14821 gattatcttc accttacgcc gcattttgtt attgattgtc accttgttcc tgctgacctt
    14881 cgttggcttc agcttgagct atttcacccc tcatgcgccg ttacaaggcg cgtcactgtg
    14941 gaatgcctgg gtgttctggt ttaacggcct gatccactgg gattttggtg tttccagcat
    15001 taacggtcaa ccgattgccg aacagttaaa agaggtcttc ccggcgacga tggaactgtg
    15061 catcctcgcc ttcggttttg cgctgattgt tggcatcccc gtggggatga ttgccgggat
    15121 tacgcgccat aagtggcagg ataacttgat caacgccatc gccttactgg gcttttcaat
    15181 cccggtgttc tggctggcgc ttctgttgac gctgttttgt tcactcacgc taggttggtt
    15241 gcccgtttca gggcgtttcg atctgctcta cgaagtgaaa ccgattaccg gttttgcgtt
    15301 gattgatgcc tggctttcgg actcaccatg gcgggatgaa atgatcatga gcgcaatccg
    15361 ccatatgata ttgcccgtga tcaccctgtc ggttgcgcca acaactgaag tgatccgcct
    15421 gatgcgtatc agcactatcg aagtgtatga ccagaactat gtaaaagcgg cggcgacccg
    15481 cggtttgtcg cgctttacta ttttgcgtcg ccacgttctg cataacgcgt taccgccggt
    15541 tattcctcgc ctgggcttac agttttccac catgctgacg ctggcgatga tcaccgaaat
    15601 ggtttttagc tggccgggtt tagggcgctg gttaattaac gccatccgcc agcaggatta
    15661 cgcagccatt tccgccggag tgatggtgtg tggctcactg gtcattattg ttaacgtgat
    15721 ttctgatatt ttgggtgcca tggctaaccc tctgaaacat aaggaatggt atgccttacg
    15781 atagcgtata cagcgaaaag cgcccgccgg gcacgctgcg taccgcctgg cgcaaatttt
    15841 atagtgacgc ctctgcgatg gtcggcctgt acggctgcgc ggggctggct gtactgtgta
    15901 tttttggcgg ctggtttgcg ccttacggca tcgaccagca atttctcggt tatcaattat
    15961 tgccgccatc atggtcgcgc tatggcgaag tttctttctt cctggggact gacgacctcg
    16021 gacgcgatgt tttaagccgt ttactgagcg gagctgcgcc aaccgtgggt ggcgcatttg
    16081 tggtgacgct tgccgcgacg atctgtggcc tagtactggg caccttcgcc ggggcgacac
    16141 acggcctccg ctcagcggtg cttaaccata ttctggatac cttactggcg atcccttcgc
    16201 tgctgttggc aattatcgtt gtagcgtttg ccggaccgag tttgtcccac gctatgtttg
    16261 ctgtctggct ggcattgctg ccgcgtatgg tacgttcgat ttacagcatg gtgcatgacg
    16321 aactggaaaa agagtacgtt atcgccgccc gtctggatgg cgcatcaacg ctgaatattc
    16381 tctggtttgc tgtgatgcca aacatcaccg ccgggctggt caccgagatc acccgcgcac
    16441 tgtcgatggc aattctcgat atcgccgcgc tgggctttct cgatctcggc gcacaactcc
    16501 cctcgcctga atggggagcg atgctcggtg atgcgctgga actgatttat gtcgccccgt
    16561 ggactgtcat gctgccaggt gcggcaatta tgattagcgt gttgttggtt aacctccttg
    16621 gcgatggcgt tcgtcgtgcg attattgcgg gggtggaata atgccattac tcgatattcg
    16681 taacctgacc attgaattta aaaccggtga tgagtgggtt aaagccgtcg accgggtaag
    16741 tatgacctta accgaaggtg aaatccgcgg tcttgttggt gaatccggtt ccggcaagag
    16801 tttgattgcg aaagcgattt gtggggtgaa taaagataac tggcgtgtta ttgctgaccg
    16861 tatgcgtttt gatgatatcg atttgctgcg tctctccgca cgcgaacggc gcaaactggt
    16921 tggccataac gtgtcgatga ttttccagga accgcagtcg tgtcttgacc cttcagaacg
    16981 tgtgggtcgc cagttgatgc aaaacatccc agcctggacc tataaaggcc gttggtggca
    17041 gcgttttggc tggcgcaaac gccgtgccat tgaactgctg caccgcgtgg ggattaaaga
    17101 tcacaaagat gcgatgcgca gttttcccta tgagttgacc gaaggtgaat gtcagaaagt
    17161 gatgatagcc attgcactgg cgaatcaacc gcgtctgctg attgctgacg aaccgaccaa
    17221 ctcaatggag ccaacaaccc aggcgcaaat ctttcgcctg ctgacgcgtc tcaaccaaaa
    17281 cagcaatacc actattttgc ttatcagcca tgacttacaa atgcttagcc aatgggcgga
    17341 taaaattaac gtgctttact gcggtcaaac agtggaaacc gcgccgagta aggagttggt
    17401 gacgatgcca catcatcctt atacccaggc gctgatccgc gcgataccag acttcggcag
    17461 cgcgatgccg cataaaagtc gcctcaatac gctgcccggc gctatcccac tgctggaaca
    17521 gttaccgatt ggttgtcgtc tggggccacg ttgcccgtat gcacaacgag aatgcattgt
    17581 gacgccacgt ttgacggggg cgaaaaatca tctctatgcc tgtcatttcc cgctgaacat
    17641 ggagaaagag tgagatgatc gaaacgctgc ttgaagtgcg taatttaagt aaaacgttcc
    17701 gctaccggac cggctggttt cgccgtcaga ccgtagaagc ggtaaaaccc ttgagcttta
    17761 cgctacgtga aggccagaca ctggcgatta ttggcgagaa tggttcgggt aaatccacgc
    17821 tggcaaaaat gctggcggga atgatagagc ctaccagcgg cgagttattg attgacgatc
    17881 atccactgca ttttggcgat tattccttcc gcagtcagcg tattcgcatg atttttcagg
    17941 atccttcgac ctcgttgaat ccccgtcaac gtatctcgca aattctggat tttccactgc
    18001 gcctgaacac cgacctggaa ccggaacagc gtcgcaaaca gattattgaa acgatgcgta
    18061 tggttgggct gctaccggat cacgtcagtt actatccgca tatgttggca cccggacaaa
    18121 aacagcgtct gggtctggcg cgcgcgctga tattgcgccc aaaagtcatt attgccgatg
    18181 aagcactcgc ttcactggat atgtcgatgc gttcgcagtt gattaatctg atgctggagt
    18241 tacaggaaaa acagggcatt tcgtatattt atgttaccca gcatatcgga atgatgaagc
    18301 acattagcga ccaggtgctg gtgatgcatc agggcaaggt tgtcgagcga ggcagcaccg
    18361 cggatgtgct ggcatcgccg ctgcatgaac tcaccaaacg gctgattgcc gggcactttg
    18421 gcgaagcatt gacagcggat gcgtggcgta aagatcgcta attttctgaa tcctccttcc
    18481 agattatcca cctcattctg acaagagatt aaatcttgtc agaatgaggt gatggataaa
    18541 ataaaatcaa atgcgcgtga tttaagacgc aatctcactt tgcaggaacg aaagctctgg
    18601 cgatatcttc gcagccgacg ttttggtgat ttcaaatttc gccgtcaaca tccggtgggg
    18661 agctacattc tcgattttgc ttgctgctcg gcgcgcgtag tcgttgagct ggatggtggg
    18721 cagcatgatt tggcagttgc ctatgatacc aggcgcacta gctggcttga gtcgcagggc
    18781 tggaccgtgc tgcgtttctg gaataacgag attgattgta atgaggaggc ggtgctggag
    18841 attattctgc aggaactgaa ccgccgatca ccctcaccct gaaagggcga gggggccgac
    18901 cgagccgaat agctgttgtg gtgaaaacat ggagactgtg ctggagaata ttcggcaagg
    18961 tctgaaccat cccagccatc gccataaaag ggttaggggc tgtatgagcc tgtttgttgc
    19021 tggggtaaca atatttgcac aatatggtcc cctcgcccct ctggggagag ggttagtgta
    19081 aggggaaaag cgcccaccct gccgcagcct gctccggtcg gacctagcaa ctatagctac
    19141 tcacagccag gttgattata ataaccattt atctgttcgt actgtttact aaaacgacga
    19201 atcgcctgat tttcaggcac aacaagcatc aacaataagg attaaagcta tgggttttct
    19261 ttccggtaag cgcattctgg taaccggtgt tgccagcaaa ctatccatcg cctacggtat
    19321 cgctcaggcg atgcaccgcg aaggagctga actggcattc acctaccaga acgacaaact
    19381 gaaaggccgc gtagaagaat ttgccgctca attgggttct gacatcgttc tgcagtgcga
    19441 tgttgcagaa gatgccagca tcgacaccat gttcgctgaa ctggggaaag tttggccgaa
    19501 atttgacggt ttcgtacact ctattggttt tgcacctggc gatcagctgg atggtgacta
    19561 tgttaacgcc gttacccgtg aaggcttcaa aattgcccac gacatcagct cctacagctt
    19621 cgttgcaatg gcaaaagctt gccgctccat gctgaatccg ggttctgccc tgctgaccct
    19681 ttcctacctt ggcgctgagc gcgctatccc gaactacaac gttatgggtc tggcaaaagc
    19741 gtctctggaa gcgaacgtgc gctatatggc gaacgcgatg ggtccggaag gtgtgcgtgt
    19801 taacgccatc tctgctggtc cgatccgtac tctggcggct tccggtatca aagacttccg
    19861 caaaatgctg gctcattgcg aagccgttac cccgattcgc cgtaccgtta ctattgaaga
    19921 tgtgggtaac tccgcggcat tcctgtgctc cgatctctct gccggtatct ccggtgaagt
    19981 ggtccacgtt gacggcggtt tcagcatcgc tgcaatgaac gaactcgaat tgaaataatc
    20041 attctgttgg taaatattgg gcggcgttct gccgcccgtt atctctgtta tacctttctg
    20101 atatttgtta tcgccgatcc gtctttctcc ccttcccgcc ttgcgtcagg ataacgattt
    20161 cctttacgac caaggagcgc ccatggaaca acgccacatc accggcaaaa gccactggta
    20221 tcatgaaacg caatccagta ctgcggagta tgacgttctg cctctggtcc cggaagccgc
    20281 aaaggtcagc gatccctttc tgctcgacgt gatccttgat gaagaaacgc tagctccctt
    20341 cctttcatgg ctggtccctg cgcgcgttct tgcagtggaa ttgttccctg accagcttac
    20401 cgtgacccgt tcacagacct tcaccgctta tgaacgcttg tctacggccc tgacggttgc
    20461 tcaggtttgc ggcgtccagc ggttatgtaa ctactattcg gcgcgactta cgccgctccc
    20521 cgggcctgat tcctctaggg aaagcaatca taggttggca caaatcacgc aatatgcccg
    20581 ccaactggct agctcgcctt ctattatcga caaccgatcg cgccagcatc tgaatgacgt
    20641 cggtcttact gccagggact gtgtaatcat taaccaaatc attggtttta ttggctttca
    20701 ggcgcggacc attgcgacat ttcaggctta tctcgggcac ccggtacgct ggttacccgg
    20761 tctggaaata caaaactacg ccgacgcgtc actgtttgct gatgaatcaa tacgctggcg
    20821 aagcagctat gaggtggaaa aactacctga agagtacaca aaaagttcaa cagcagaact
    20881 ttgccaactg gccgaaacac tctctctcca ccctatttca ctttcccttc tcgaaaggtt
    20941 gttaaacagt acacgggtta atacacagcc ggataataag cttgcggcgt tgttatgcgc
    21001 gcggataaat ggcagtcctg cttgttttgc cgcctgtatg gattcatcaa atgaatataa
    21061 aaaaatcagc ccccttctgc gcaagggcga aaatgaaatt aaccaatggg ctgaccgtca
    21121 ttctgttgag cacgctaccg ttcaggcgat acaatggctg acccgagcac ccgatcgctt
    21181 tagcaccgcc cagttcagcc ctttactcga acacgaacaa tcatcaacgc agattattaa
    21241 tctgctggta tggagcgggc tgtgtggctg gataaatcgc ttaaaaatcg cgttgggtga
    21301 gacatattaa ccttgccgcg tcagacagat tcgcgtaaaa ctgtcagccg ctctaatggc
    21361 caccaaaata gacaattatg tttcaggaca acccgctgct agcgcagctt aaacagcaac
    21421 tgcattccca gacgccacgc gctgaagggg tggtaaaagc cacagaaaaa ggctttggct
    21481 tcctggaagt cgacgcgcaa aaaagttatt tcattccgcc gccgcagatg aaaaaagtca
    21541 tgcatggcga ccgaattatc gcggtgatcc acagtgaaaa agaacgtgaa tccgcagagc
    21601 cagaagaact ggttgaaccg ttcctgactc gtttcgtggg taaagttcag ggcaaaaatg
    21661 accgtctggc catcgttcct gatcatccac tcttaaaaga cgccattcct tgccgcgcag
    21721 cccgtggcct gaaccacgag tttaaagaag gcgactgggc ggttgccgaa atgcgccgtc
    21781 atccgctgaa aggcgatcgt tctttctatg ctgaattgac acaatacatc acttttggtg
    21841 acgaccactt tgtaccgtgg tgggttacac ttgcgcgcca taatctggaa aaagaagcac
    21901 cagacggcgt cgctaccgaa atgctcgatg aaggtctggt tcgtgaagat ctgaccgcgc
    21961 tggattttgt caccatcgac agtgccagca cagaagatat ggatgacgcc cttttcgcta
    22021 aggcgttgcc ggatgacaaa cttcagctga ttgtggcgat tgccgatcca accgcgtgga
    22081 ttgctgaagg cagcaagctg gacaaagccg cgaaaattcg cgcattcacc aactatctgc
    22141 ctggcttcaa catccctatg ctgcctcgcg agctttctga cgatctctgc tcactgcgcg
    22201 ccaatgaagt ccgcccggta ctggcatgcc gcatgacgct ctccgctgat ggcgccattg
    22261 aagataatat cgaattcttt gccgccacca tcgaatccaa agcaaagctg gtgtatgacc
    22321 aggtttctga ctggctggaa aataccggtg actggcagcc tgaaagtgaa gcaattgccg
    22381 aacaagtccg tttgctagcg caaatttgcc aacgccgcgg cgagtggcgt cataaccacg
    22441 cactggtgtt taaagatcgc ccggattacc gctttattct cggtgaaaaa ggtgaagtgc
    22501 tggatatcgt cgccgagcct cgtcgcattg ccaaccgtat cgtcgaagaa gcgatgattg
    22561 ccgctaacat ttgtgcggcc cgcgtactgc gcgataagct cggttttggc atctataacg
    22621 tgcatatggg ctttgatccg gcgaatgccg acgcgctggc agcgttgctg aaaacgcacg
    22681 gtctgcatgt cgatgccgaa gaagtgctca cgctggacgg tttctgcaaa ctgcgtcgtg
    22741 aactggacgc gcaaccaact ggtttcctcg acagccgcat tcgtcgcttc cagtcatttg
    22801 ctgaaattag cactgaaccc ggtcctcact ttggcctcgg tctggaagca tacgccacct
    22861 ggacttcgcc gatccgtaaa tatggcgaca tgatcaacca ccgtctgctg aaagcggtta
    22921 tcaaaggcga aactgcgacg cgcccacagg atgaaatcac tgtccaaatg gccgagcgtc
    22981 gccgtctcaa ccggatggca gaacgtgatg ttggtgactg gttatacgca cgcttcctga
    23041 aagacaaagc cgggaccgac acccgtttcg cagcggaaat tgtcgatatc agccgtggcg
    23101 gcatgcgtgt gcgtttggtt gataacggcg ctatcgcctt tattccggca cctttcttac
    23161 acgctatgcg cgatgaactg gtttgcagcc aggaaaacgg cactgtacaa attaaaggtg
    23221 aaacggttta caaagtaact gacgttattg acgtcaccat tgccgaagtc cgcatggaaa
    23281 cccgcagcat tattgcgcgc ccggtcgcgt aatctccttt cacggcctat tcctcatgga
    23341 tgggccgttt atttccccgc tctaccttcg ttatatccag gcagattttt taactgactt
    23401 tcgtttgaaa actggcgttt ttccaaaact ggattacaaa taaatatata aaaaaacaat
    23461 gcgtttagcc gccgttaatt tcatgtaagt ca
//
LOCUS       DADLFA010000049        23030 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000049 DADLFA010000000
VERSION     DADLFA010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23030
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.049"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..562)
                     /locus_tag="JQH65_001630"
     CDS             complement(<1..562)
                     /locus_tag="JQH65_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY6139084.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGG"
     gene            complement(781..1758)
                     /gene="epmA"
                     /locus_tag="JQH65_001631"
     CDS             complement(781..1758)
                     /gene="epmA"
                     /locus_tag="JQH65_001631"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HAY6139085.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLNLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            2083..3891
                     /gene="frdA"
                     /locus_tag="JQH65_001632"
     CDS             2083..3891
                     /gene="frdA"
                     /locus_tag="JQH65_001632"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HAY6139086.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRMAGEQATERAATAGNGNEEAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3884..4618
                     /locus_tag="JQH65_001633"
     CDS             3884..4618
                     /locus_tag="JQH65_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HAY6139087.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4629..5024
                     /gene="frdC"
                     /locus_tag="JQH65_001634"
     CDS             4629..5024
                     /gene="frdC"
                     /locus_tag="JQH65_001634"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HAY6139088.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPDAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            5035..5394
                     /gene="frdD"
                     /locus_tag="JQH65_001635"
     CDS             5035..5394
                     /gene="frdD"
                     /locus_tag="JQH65_001635"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HAY6139089.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5457..6590
                     /gene="blaEC"
                     /locus_tag="JQH65_001636"
     CDS             5457..6590
                     /gene="blaEC"
                     /locus_tag="JQH65_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HAY6139090.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQMPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6679..7212
                     /locus_tag="JQH65_001637"
     CDS             6679..7212
                     /locus_tag="JQH65_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HAY6139091.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDGGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(7209..7526)
                     /locus_tag="JQH65_001638"
     CDS             complement(7209..7526)
                     /locus_tag="JQH65_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound-resistance protein
                     SugE"
                     /protein_id="HAY6139092.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7708..7854)
                     /gene="ecnB"
                     /locus_tag="JQH65_001639"
     CDS             complement(7708..7854)
                     /gene="ecnB"
                     /locus_tag="JQH65_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HAY6139093.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7965..8090)
                     /gene="ecnA"
                     /locus_tag="JQH65_001640"
     CDS             complement(7965..8090)
                     /gene="ecnA"
                     /locus_tag="JQH65_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HAY6139094.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(8142..8708)
                     /gene="efp"
                     /locus_tag="JQH65_001641"
     CDS             complement(8142..8708)
                     /gene="efp"
                     /locus_tag="JQH65_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAY6139095.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8750..9799
                     /gene="epmB"
                     /locus_tag="JQH65_001642"
     CDS             8750..9799
                     /gene="epmB"
                     /locus_tag="JQH65_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HAY6139096.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRVGVTLLNQSVLLRDVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQLLIKQGS"
     gene            10173..11042
                     /locus_tag="JQH65_001643"
     CDS             10173..11042
                     /locus_tag="JQH65_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6139097.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEVEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(11245..11598)
                     /locus_tag="JQH65_001644"
     CDS             complement(11245..11598)
                     /locus_tag="JQH65_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HAY6139098.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(11736..13382)
                     /gene="groL"
                     /locus_tag="JQH65_001645"
     CDS             complement(11736..13382)
                     /gene="groL"
                     /locus_tag="JQH65_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAY6139099.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(13426..13719)
                     /locus_tag="JQH65_001646"
     CDS             complement(13426..13719)
                     /locus_tag="JQH65_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004391824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAY6139100.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            13995..15251
                     /gene="yjeH"
                     /locus_tag="JQH65_001647"
     CDS             13995..15251
                     /gene="yjeH"
                     /locus_tag="JQH65_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HAY6139101.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAAQVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPCAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRSASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIVLAGLWLLLPKRKTPENGIIT"
     gene            complement(15267..15743)
                     /gene="fxsA"
                     /locus_tag="JQH65_001648"
     CDS             complement(15267..15743)
                     /gene="fxsA"
                     /locus_tag="JQH65_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HAY6139102.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            16080..17516
                     /gene="aspA"
                     /locus_tag="JQH65_001649"
     CDS             16080..17516
                     /gene="aspA"
                     /locus_tag="JQH65_001649"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HAY6139103.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            17634..18935
                     /locus_tag="JQH65_001650"
     CDS             17634..18935
                     /locus_tag="JQH65_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HAY6139104.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            19051..19389
                     /gene="cutA"
                     /locus_tag="JQH65_001651"
     CDS             19051..19389
                     /gene="cutA"
                     /locus_tag="JQH65_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HAY6139105.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            19365..21062
                     /locus_tag="JQH65_001652"
     CDS             19365..21062
                     /locus_tag="JQH65_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HAY6139106.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFTFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNHALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NITANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            21099..21674
                     /locus_tag="JQH65_001653"
     CDS             21099..21674
                     /locus_tag="JQH65_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY6139107.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKQLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            21781..21856
                     /locus_tag="JQH65_001654"
     tRNA            21781..21856
                     /locus_tag="JQH65_001654"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21814..21816,aa:Phe,seq:gaa)
     gene            22054..>23030
                     /locus_tag="JQH65_001655"
     CDS             22054..>23030
                     /locus_tag="JQH65_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HAY6139108.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGTGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQTVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPISENVMNAS"
BASE COUNT         5740 a         5943 c         5698 g         5649 t
ORIGIN      
        1 gcccaccatt taataacaaa atagtaatgc ttactaacag caatactaaa ttcagacaca
       61 ttactgcaat accgcccacc gcagtaatgc gggcaatttt attaatcccc tttgaagcaa
      121 cgacggtgac cagaatcatc catgctactg ccagcagacc aaccacctgc gtaggctcca
      181 gtccggcaat acgccagtgc tgggtcatgt cgctaccata gaggaatgtt gagaacggta
      241 cccaaacttt cgcggaggta ctcaccatcc agatgatata agaggaaaac cacataaacg
      301 taccaatgaa ggcaaaacgt ggtccgacac tattattcat ccaggaatag ataccacctt
      361 cttctttgcg ataagcagct cccatttcag ccatcattaa ggcgaatgga ataaagaata
      421 acaatgcaga aaatatataa aagggaatcg cactataacc cattaagtaa taagccgatg
      481 ggctattggc aaatccaaat acggaagtaa agatcatcaa tatgagtcct atcagactca
      541 tcttttttat cgtgtgaggc atgaaaccat ccttcatttc agcgcgtgta taacacgtgc
      601 ccactgaact gcactgcgca acattgagca ggcagcgtaa aaaccaatac agaattgata
      661 cccggatgat atcaaatatc cagtcttaaa ttgtggcctt taacggaaaa atgaaaacat
      721 atgctacaga atggcgctta tcacgccatt cttcgctgtt aattcagtaa tttttcagaa
      781 ttatgcccgg tcaacgctaa aggcgatgac ttcagccagt gtctccgcgc ccagcgccaa
      841 catcaccaga cgatcaacac ctaatgccac gccggaacag tcaggcatac cgactttcaa
      901 ggcttcaatc agattctggt caatggggtg ctgcggcaaa ccgcgcgccg cgcgcttacg
      961 gttatcttgt tcaaagcgtt gttgctgctc acgggcatcc gttaattcat ggaaaccatt
     1021 cgccagctca atacctttat aataaacctc aaagcgttca gcgacccgat gatcttcggt
     1081 actgatttgc gccagtgatg cctggctggc tggaaagtgg tacacaaagg tcggtttttc
     1141 tttgccaata tttggctcta cgccaaaggt aaacagcaat tgtagcaacg tgtcgcggtc
     1201 ttcttcggta tcagcaacgt tactcaaatt cagtttcgct gcgacttccc gcagttgcgt
     1261 tttgtcggca gagagcgggt caatttccag ataacgcaag aaagcttgtt gataagaaag
     1321 gctttctgct gccgggcagt ccagcacctg ttgtaagaga tcgtccacct cgttcatcaa
     1381 ccggtacata tcatagtgcg gtcgatacca ctcaagcata gtgaactcag ggttgtgata
     1441 acgccccatc tcttcattac ggaagctgcg gcacagctgg aataccggcc cacaaccggc
     1501 aaccagcagg cgtttcatat ggtattccgg gctggtcatt aaccagagat tcatcccctg
     1561 cgaatgcccg gggccaacga aacgtgtctc aaacgggacc aaatgaatat cggttaccgt
     1621 cgcctggctc atacaaggcg tctccacctc cagcactcca cgatcagcaa agaaacgacg
     1681 gatctccgcc ataatcgccg cgcgttttaa taagttagga atggatgcgc tcggctgcca
     1741 ggatgccgtt tcgctcatag ttaaatctcc agtttttgac aagggcacga agtctactcg
     1801 caacgcgacg gcgagacaaa ttttacgcag gaatcaaaca gcggtgggca gtgactaaaa
     1861 aaagcacgat ctgatggttt agtaattaaa ttaatcatct tcagtgataa tttagccctc
     1921 ttgcgcacta aaaaaatcga tctcgtcaaa tttcagactt atccatcaga ctatactgtt
     1981 gtacctataa aggagcagtg gaatagcgtt cgcagaccgt aactttcagg cacttaccct
     2041 gaagtacggg gctgtgggat aaaaacaatc tggaggaatg tcgtgcaaac ctttcaagcc
     2101 gatcttgcca ttgtaggcgc cggtggcgcg ggattacgtg ctgcaattgc tgccgcgcag
     2161 gcaaatccaa atgcaaaaat cgcactaatc tcaaaagtat acccgatgcg tagccatacc
     2221 gttgctgcag aagggggctc cgccgctgtc gcgcaggatc atgacagctt cgaatatcac
     2281 tttcacgata cagtagcggg tggcgactgg ttgtgtgagc aggatgtcgt ggattatttc
     2341 gtccaccact gcccaaccga aatgacccaa ctggaactgt gggggtgccc atggagccgt
     2401 cgcccggatg gtagcgtcaa cgtacgtcgc ttcggcggca tgaaaatcga gcgcacctgg
     2461 ttcgccgccg ataagaccgg cttccatatg ctgcacacgc tgttccagac ctctctgcaa
     2521 ttcccgcaga tccagcgttt tgacgaacat ttcgtgctgg atattctggt tgatgatggt
     2581 catgttcgcg gcctggtagc aatgaacatg atggaaggca cgctggtgca gatccgtgct
     2641 aacgcggtcg ttatggctac tggcggtgcg ggtcgcgttt atcgttacaa caccaacggc
     2701 ggcatcgtta ccggtgacgg tatgggtatg gcgctaagcc acggcgttcc gctgcgtgac
     2761 atggaattcg ttcagtatca cccaaccggt ctgccaggtt ccggtatcct gatgaccgaa
     2821 ggctgccgtg gtgaaggcgg tattctggtc aacaaaaatg gctaccgtta tctgcaagat
     2881 tacggcatgg gcccggaaac tccgctgggc gagccgaaaa acaaatatat ggaactgggt
     2941 ccacgcgaca aagtttctca ggccttctgg cacgaatggc gtaaaggcaa caccatctcc
     3001 acgccgcgtg gcgatgtggt ttatctcgac ttgcgtcacc tcggcgagaa aaaactgcat
     3061 gaacgtctgc cgttcatctg cgaactggcg aaagcgtacg ttggcgtcga tccggttaaa
     3121 gaaccgattc cggtacgtcc gaccgcacac tacaccatgg gcggtatcga aaccgatcag
     3181 aactgtgaaa cccgcattaa aggtctgttc gccgtgggtg aatgttcctc tgttggtctg
     3241 cacggtgcaa accgtctggg ctccaactcc ctggcggaac tggtggtctt cggccgtatg
     3301 gccggtgaac aagcgacaga gcgtgcagca actgccggta atggcaacga agaggcaatt
     3361 gaagcgcagg cagctggcgt tgaacaacgt ctgaaagatc tggttaacca ggatggcggc
     3421 gaaaactggg cgaagatccg cgacgaaatg ggcctagcaa tggaagaagg ttgcggtatc
     3481 taccgtacgc cggaactgat gcagaaaacc atcgacaagc tggcagagct gcaggaacgc
     3541 ttcaagcgcg tgcgcatcac cgacacctcc agcgtgttca acaccgacct gctctacacc
     3601 attgaactgg gccacggtct gaacgttgct gaatgtatgg cgcactccgc aatggcacgt
     3661 aaagagtccc gcggcgcaca ccagcgtctg gacgaaggtt gcaccgagcg tgacgacgtc
     3721 aacttcctca aacacaccct cgccttccgc gatgctgatg gcacgactcg cctggagtac
     3781 agcgacgtga agattactac gctgccgcca gctaaacgcg tttacggtgg cgaagcggat
     3841 gcagccgata aggcggaagc agccaataag aaggagaagg cgaatggctg agatgaaaaa
     3901 cctgaaaatt gaggtggtgc gctataaccc ggaagtcgat accgcaccgc atagcgcatt
     3961 ctatgaagtg ccttatgacg caactacctc attactggat gcgctgggct acatcaaaga
     4021 caacctggca ccggacctga gttaccgctg gtcctgccgt atggcgattt gtggttcctg
     4081 cggcatgatg gttaacaacg tgccaaaact ggcatgtaaa accttcctgc gtgattacac
     4141 cgacggtatg aaggttgaag cgttagctaa cttcccgatt gaacgcgatc tggtggtcga
     4201 tatgacccac ttcatcgaaa gtctggaagc gatcaaaccg tacatcatcg gcaactcccg
     4261 caccgcggat cagggtacta acatccagac cccggcgcag atggcaaagt atcaccagtt
     4321 ctccggttgc atcaactgtg gtctgtgcta cgccgcgtgc ccgcagtttg gcctgaaccc
     4381 agagttcatc ggtccggctg ccattacgct ggcgcatcgt tataacgaag atagccgcga
     4441 ccacggtaag aaggagcgta tggcgcagtt gaacagccag aacggcgtat ggagctgtac
     4501 tttcgtgggc tattgctccg aagtctgccc gaaacacgtc gatccggctg cggccattca
     4561 gcagggcaaa gtagaaagtt cgaaagactt tcttatcgcg accctgaaac cacgctaagg
     4621 agtgcaacat gacgactaaa cgtaaaccgt atgtacggcc aatgacgtcc acctggtgga
     4681 aaaaattgcc gttttatcgc ttttacatgc tgcgcgaagg cacggcggtt ccggctgtgt
     4741 ggttcagcat tgaactgatt ttcgggctgt ttgccctgaa aaatggcccg gacgcctggg
     4801 cgggattcgt cgacttttta caaaacccgg ttatcgtgat cattaacctg atcactctgg
     4861 cggcagctct gctgcacacc aaaacctggt ttgaactggc accgaaagcg gccaatatca
     4921 ttgtaaaaga cgaaaaaatg ggaccagagc caattatcaa aagtctctgg gcggtaactg
     4981 tggttgccac catcgtaatc ctgtttgttg ccctgtactg gtaaggagcc tgagatgatt
     5041 aatccaaatc caaagcgttc tgacgaaccg gtattctggg gcctcttcgg ggccggtggt
     5101 atgtggagcg caattattgc gccggtgatg atcctgctgg tgggtattct gctgccactg
     5161 gggctgtttc cgggtgatgc gctgagctac gagcgcgttc tggcgttcgc gcagagcttc
     5221 attggtcgcg tattcctgtt cctgatgatc gttctgccgc tgtggtgtgg tttacaccgt
     5281 atgcaccacg cgatgcacga tctgaaaatt cacgtgcctg cgggcaaatg ggttttctac
     5341 ggtctggctg ctatcctgac agttgtcacg ctgattggta tcgttacaat ctaacgtatc
     5401 gccaatgtaa atccggcccg cctatggcgg gccgttttgt atggaaacca gaccttatgt
     5461 tcaaaacgac gctctgcgcc ttattaatta ccgcctcttg ctccacattt gctgcccctc
     5521 aacaaatcaa cgatattgtg catcgcacaa ttaccccgct tatagagcaa caaaagatcc
     5581 ccggtatggc ggtggcggta atttatcagg gtaaacctta ttactttacc tggggctatg
     5641 cggacatcgc caaaaagcag cccgtcacac agcaaacgtt gtttgagtta ggttcggtca
     5701 gcaaaacatt tacgggcgtg cttggtggcg atgctattgc tcgaggggaa atcaagttaa
     5761 gcgatcccgc aacaaaatac tggcctgaac ttaccgctaa acagtggaat gggatcacac
     5821 tattacatct tgcgacctac accgctggcg gcctgccatt gcagatgccg gatgaagtga
     5881 aatcctcaag cgacttgctg cgcttctatc aaaactggca gcctgcatgg gctccaggaa
     5941 cacaacgtct gtatgccaac tccagtatcg gtttgttcgg cgcactggct gtgaagccgt
     6001 ctggtttgag ttttgagcag gcgatgcaaa ctcgtgtctt ccagccactc aaactcaacc
     6061 atacgtggat taatgtaccg cccgcagaag aaaagaatta cgcctgggga tatcgcgaag
     6121 gcaaggcagt tcatgtttcg ccaggggcgt tagatgctga agcttatggt gtgaagtcga
     6181 ccattgaaga tatggcccgc tgggtgcaaa gcaatttaac cccccttgat atcaatgaga
     6241 aaacgcttca acaagggata caactggcac aatctcgcta ctggcaaacc ggcgatatgt
     6301 atcagggcct gggctgggaa atgctggact ggccggtcaa tcctgacagc atcattaacg
     6361 gcagtgacaa taaaattgca ctggcagcac gcccggtaaa agcgattaag cccccaactc
     6421 ctgcagtacg cgcatcatgg gtacataaaa cgggggcgac cggcggattt ggtagctatg
     6481 tcgcgtttat tccagaaaaa gagctgggta tcgtgatgct ggctaacaaa aactatccca
     6541 atccagcgag agtcgccgcc gcctgtcaga ttctcaacgc tctacagtaa aatttcatcg
     6601 ggtccgaatt ttcggacctt ttctgcgctt ttccttgctg tcatctacac ttagaaaaaa
     6661 accagtaagg aaacatttat gcgcctgctc cctctcgttg ccgcagcgac agctgcattt
     6721 ctggtcgttg cctgcagttc tcctacgccg ccgcgtggcg tgacagtagt gaataatttc
     6781 gacgctaaac gctatctcgg tacctggtat gagattgccc gttttgatca ccgttttgaa
     6841 cgtggactgg aaaaagtcac cgcaacatac agtctgcgtg atggcggcgg cctgaatgtc
     6901 atcaataaag gctataaccc tgacagagaa atgtggcagc agagtgaagg gaaagcgtac
     6961 tttaccggcg caccaacacg cgctgcgctg aaagtgtcat tctttggtcc tttctatggc
     7021 ggttataacg tcattgcact tgatcgggaa taccgccatg cgctggtttg cggcccggac
     7081 cgcgactacc tgtggatact ctcccgcacg ccaaccattt ctgacgaagt gaaacaggag
     7141 atgctggcag tcgcgacccg ggaagggttt gatgtcagta agtttatttg ggtacagcag
     7201 cccggtagtt agtgagtgct gagtttcaga ccaataatcc ccaatacgat taacgccaga
     7261 ctcgccaggc gcatcgggtt agcggactca ccgagcagca caatgccggt gatagccgcg
     7321 ccgactgcgc caatacccgt ccacacggca taagccgtcc ctactggtaa cgatttcatc
     7381 gcccaggcaa gtagcgccat actgacaatc atcgccgtca cggtaataac actcggcgtc
     7441 aaacgactaa agccgtgggt atatttcagg ccaacggccc ataccacttc cagcagacca
     7501 gcaataacta agataatcca ggacatatca ggctccggag aattggggcc gtccccggtg
     7561 aaaagacgcg tttgcaggtc gtcctgcaaa gctgatggtt gaacggatat tttgccaggt
     7621 ggcaggggaa tttcaactat taccttagtg actatcgcag aaaaaacaaa cggcacgaca
     7681 cagctgtcgt accgttattg cttattgcta ttgctgcgct ttcgttgctg cgccagaaat
     7741 cgcgttaccg ccatcagaaa tgtcttcacc aacgccacgc gtggtgttgc aggcagttaa
     7801 tactgttgaa agcaccagaa cagaaaagat cgctgcaatt gtcttcttca ccataacgtc
     7861 ttccttttag gtaatgttat ttatgtttgc ctatagcaaa ttaataatag acaacatcgc
     7921 caaaaatgac ggaaatctga tgatttttcg gaagagaaga aaaattagct ggcagcgcga
     7981 gagatggagt tgccgagatg tttgatgtct tcgccgaaac cgcgagcggt gttacagccc
     8041 gtgagcagcg tactggcaag caaaacaaga acgataaggc gtttcatcat ctctgacctg
     8101 aataagtgat ggtgcagcct gcaggccgca ccacaaccgc attacttcac gcgagagacg
     8161 tattcaccag agcgggtatc cactttgatg acttcgccga tttgtacgaa cagcggaacc
     8221 ttaaccacag cgccagtaga cagggtagcc ggtttgccgc cagtacctgc ggtatcacct
     8281 ttcaggcccg gatcggtatc aacgatttcc agttcaacga agttcggcgg agtaacggag
     8341 atcggctgac cattccacag agttacgata cactctgcct gatccagcag ccatttagcg
     8401 ttatcgccaa ttgcttttgc atcagcagac agctgctcga aagtttcgtt gttcatgaag
     8461 tgccagaact caccgtcgtt gtacaggtaa gtcaggttca tatcgacaac atcagcgcct
     8521 tcagcggaat cagtagattt gaaggttttt tctacgcgag taccggtcag cagacgacgc
     8581 agtttaacgc gagcaaatgc ctggccttta cccggtttta cgaattcact cgcttcaacc
     8641 gcgtaaggtt cgccgtctaa catgatttta agaccagcac gaaaatcgtt gctatagtac
     8701 gttgccataa ggccctctga aatttgttaa ttggtagcta agccacaaaa tggcgcatat
     8761 tgtaacccta aataccccat ccagagaaga ttggttaacg caacttgccg atgttgtgac
     8821 cgatcctgat gaacttctgc gtcttttgaa tatagacgcg gacgaaaaac tgttggccgg
     8881 acgcagcgcc aaaaagctgt ttgccctgcg tgtgccccgc tcatttatcg atcgcatgga
     8941 gaaaggcaat ccggacgatc ctcttttgcg tcaggtactt acctcgcaag atgagtttgt
     9001 cgtcgcgtcc ggattctcca ccgacccact ggaagaacag cacagcgtag tgcctggttt
     9061 gttgcataaa taccacaacc gggcgctttt gctggtcaaa ggcggctgcg cggtaaattg
     9121 ccgctattgc ttccgtcgcc acttccccta tgccgaaaat cagggcaaca agcgtaactg
     9181 gcagactgca cttgagtatg ttgctgcgca tccggaactg gacgagatga ttttctccgg
     9241 cggcgatccg ctgatggcga aagatcacga gctggactgg ttgctcacac aactggaagc
     9301 catcccgcat atcaaacgtc tgcggattca cagtcgtctg ccgattgtga tcccggcacg
     9361 catcacagat gcgctggttg aacgcttttc acattctacg ctgcaaatcc tgctggtgaa
     9421 tcacatcaac catgccaatg agatagatga aacattccgt caggcgatgg ctaagttgcg
     9481 ccgggtaggc gttactttgc tgaaccagag cgttctgtta cgtgatgtga acgataacgc
     9541 acaaacgctg gcaaacctga gtaatgcgtt gttcgatgcc ggcgtgatgc cgtattacct
     9601 gcatgtgctc gataaagtac agggcgcagc gcattttatg gtgagtgatg acgaagcacg
     9661 gcagattatg cgtgagttgc tgacattggt gtcgggatat ctggtgccga aactggcgcg
     9721 agaaattggc ggcgaaccca gcaaaacgcc gctggatctc cagctacgcc agcagttact
     9781 gattaaacag ggaagttaaa cattaaaatt gcctgatgcg ctacgcttat caggcctaca
     9841 tcgtgcattg aaattgaatg gatttgcaat gaactgtagg ccggataagg cgtttacgcc
     9901 gcatccggca agagcaacaa acgcaagtac agcaacctgt cattaaactg agaaatttaa
     9961 tctttgcccg ttgcataacc tttaattatt catatgcatt gctgttgtta ctttttatat
    10021 agctaacacc aataattaac ctttcttttc tgtttatata gagtgagcac ggctatttat
    10081 gttattttaa caactattac taccaagccc atttatctgg cttataaatt aaaattaatt
    10141 atttaattga gaaatggtta gggagaacct acatggccat aagcatcaag ggtgttaata
    10201 ccggtgttat tcgcaagagt aataatttca ttgcactggc gctgaaaatt aaagaacctc
    10261 gcaataaaga atcgttgttc tttatgtctg tcatggaact gcgcgacctg ctgattgcgc
    10321 tggaaagtcg tctgcatcaa aagcataaac tggatgctgc tgcacgcctt cagtatgagc
    10381 aagctcgcga taaagtcatt aagaaaatgg cagaaaacat cccagaaatt ctggttgatg
    10441 agcttaaaaa tgccgatatt aatcgtcggg ttaacacgct ggaacttacc gataatcaag
    10501 gcgaaaatct taccttcgta ttaacgctac atgacggtag caaatgtgag ttagtcgtca
    10561 atgagttgca gattgaaatg ctagcgcgag caatcattca tgcaattaat aatgcggaaa
    10621 tgcgtgaact ggcattacgt attacctcac tgttagattt cctgccgtta tatgacgttg
    10681 actgccagga aaatggcaat ctggaatatg acacctattc ccaaccggaa tggaaacata
    10741 acttgttcga ccattatctg gcggtgcttt accgctttaa agatgaaagt ggcaaagaac
    10801 aatttagcgg cgcagtggtg aaaacgcgtg aagcaacgcc gggtaaagaa gtagaggcga
    10861 tcactcgccg aatgcttgat ttcagcccac gactgaaaaa actcgcgggt gtgccatgtc
    10921 aggtctatgt tcgcaccgta gcggcgaata acgcccaacc gctaacccag gatcaatgct
    10981 tacgcgcgct gcatcactta cgtgttcaat ccaccagcaa aactgcacca caggcgaaat
    11041 aatctcatct ttgctaatgc gaggctggag ccatccagcc tgaaatgact gataacgtga
    11101 tcgttggtca tgccggatgc ggcgtaaacg ccttatccgg cctacaaatt ctcaagcatt
    11161 caatcagttg caatgcctgc tgtaggcctg ataagcgaag cgcatcaggc aataatacgt
    11221 ttgtcaccca gcctctggat cacatcagtt cgggcactta taaacctgac cgataatctg
    11281 gctatccgtc gggacaaaac tggacaacat tccctgcgac gggctgctga tgccataaat
    11341 cacgttaccg cccattgcag ccgcctggtt gcgcagatcg tttgctgcgc cgcgcataga
    11401 accgccctct tctccgtgtt gcccggaaag ccagttgctt tgcttacctg tcgcagtacc
    11461 aatcagctgg cactctgcgc ctggctgctc gtccacaatg cgtacactct gaccggcagc
    11521 actcaattcg ttgctggagc tacaacccgc catcagtagc gcggcaccga caatccctgc
    11581 taagtatttt acgtgcatgt tattccccat aatcaatgag ctggacgcca ctcgcgtcgt
    11641 ccgtgtctga atcttatact agaaagatga ccaaaagaaa aacccccaga catttctgcc
    11701 cgggggtttg tttatttctg cgaggtgcag ggcaattaca tcatgccgcc catgccaccc
    11761 atgccgccca taccgccagc agcgcctaag tcagctgcat cgtttttcgg caggtcggta
    11821 accatgcact cggtggtgat catcaggcca gccacagaag ccgcgtactg cagagcagaa
    11881 cgggttactt tggttggatc caagataccc atgtcgatca tgttgccgta ttcttcggtt
    11941 gctgcgttgt aaccgtagtt gccgtcgccg cctttaacag tgttagcaac aacagacggt
    12001 tcttcgccgc agttcaatac gatctgacgc agcggagctt ccattgcacg cagtgcaact
    12061 ttgataccca cgttctggtc ttcgttctga ccacgcaggt cagccagttt agacgctacg
    12121 cggatcagcg caacaccacc accagcaacc acgccttctt ctaccgcagc acgggtcgcg
    12181 tgcagggcat cttcaacgcg tgcttttttc tctttcattt caacttcggt agcagcacct
    12241 actttgataa ctgcaacgcc gcctgccagt ttcgctacgc gctcctgcag tttttcacgg
    12301 tcgtagtcag aagttgcttc ttcaatctgc tgacggatct gagcaacacg gccctggatt
    12361 gcagcttctt cacccacgcc atcaatgatg gtggtggtgt ctttgttgat cacaacacgt
    12421 ttagcctgac ccaggtcttc cagggttgct ttttccagct ccataccgat ctcttcagag
    12481 attacggtac cgccagtcag ggttgcgata tcctgcagca tagctttacg gcgatcgccg
    12541 aagcccggag ctttaaccgc agcaactttc acgatgccac gcatggtgtt aacaaccaga
    12601 gttgccagcg cttcgccttc aacatcttca gcgatgatca gcagcggttt acctgcttta
    12661 gcaacggctt ccagaaccgg cagcatttcg cggatgttgg agattttctt gtcagccagc
    12721 aggatgaacg ggctttccag ttctactgcg ccagtttccg gcttgttgat gaagtaagga
    12781 gacaggtagc cacggtcgaa ctgcatacct tcaaccacgt ccagttcgtc ctgcagaccg
    12841 gtaccgtctt caacggtgat aacgccttct ttaccgactt tgtccatcgc ttcagcgatc
    12901 agtttaccta cggtttcgtc ggagttagcg gagatggtac caacctgagc aatcgcttta
    12961 gagtcagagc atggtacgga cagcgctttc agttcttcaa ctgcagcggt aacagctttg
    13021 tcgataccac gtttcaggtc catcgggttc atgcccgcag caactgcttt cagaccctca
    13081 gtgatgatag cctgagccag tacggttgca gtggtggtac cgtcgcctgc agcgtcgttc
    13141 gctttagagg caacttcttt caccatctgc gcacccatat tttcgaactt gtcttccagt
    13201 tcgatttcac gagcaacgga aacaccatct ttggtgatgg tcggtgcacc gaaagattta
    13261 tccagaacta cgttacggcc tttcggaccg agggtaactt tcactgcatc tgccagtacg
    13321 tttacgccgc gcagcatttt cacacgagcg tcgttaccga attttacgtc tttagctgcc
    13381 attatcttta ttccttaaat tcgtatgttc agtgtcgtgc gaggattacg cttcaacaat
    13441 tgccagaatg tcgctttcgg acatgatcaa cacttcttca ttgtcgatct tctcagattt
    13501 cacaccgtag ccatcgttga aaataacgat gtcgccaact ttcacatcca gcggcttcac
    13561 ttcgccattt tcaaggatac ggccattgcc gacagccagc acttcgccgc gggtggattt
    13621 agccgctgca gagccggtca gaacgatgcc gccagcagat ttagtttcaa cttctttacg
    13681 cttgacgatc acgcgatcat gcaatggacg aatattcatt gataactctc ctttgagaaa
    13741 gtccgtatct gttatgggtg acgccggagc ttacgtggtt tcccggctgg tgaccagaga
    13801 aatggggatg aggattcgcc ccttcaaggg ggaaaaagaa aaaaaattct gatcagcaca
    13861 aaatcgggtg aaaaccctga ttcacctcac atttcatcgc aatttcttca tcgccgtcat
    13921 aagcgaatct gattgtgcta ccatcgaaaa tctacgcagt tgcccaaaat ttgggcgcaa
    13981 tcggatatca acccatgagt ggactcaaac aagaactggg gctggcccag ggcattggcc
    14041 tgctatcgac gtcattatta ggcaccggcg tgtttgccgt tcctgcgtta gctgcacagg
    14101 tagcaggcaa taacagcctg tgggcgtggc ccgttttgat tatcttagtg ttcccgattg
    14161 cgattgtgtt tgcgattctg ggtcgccact atccctgcgc aggcggtgtc gcgcacttcg
    14221 tcggtatggc gtttggttcg cggcttgagc gagtcaccgg ctggttgttt ttatcggtca
    14281 ttcccgtggg tttgccagcc gcgctacaaa ttgccgccgg gttcggccag gcaatgtttg
    14341 gctggcatag ctggcaactg ttgttggcag aactcggtac gctggcgctg gtatggtata
    14401 tcggtactcg cagtgccagt tccagtgcta atctacaaac cgttattgcc ggacttatcg
    14461 tcgcgctgat tgtcgctatc tggtgggcgg gcgatatcaa acctgcgaat atccccttcc
    14521 cggcacctgg taatatcgaa cttaccgggt tatttgctgc gttatcagtg atgttctggt
    14581 gttttgtcgg tctggaagca tttgcccatc ttgcctcgga atttaaaaat ccagagcgtg
    14641 attttcctcg cgcattgatg attggtctgc tgctggcagg attagtctac tggggctgta
    14701 cggtagtcgt cttacacttc gacgcctatg gtgaaaaaat ggcggcggca gcatcgcttc
    14761 caaaaattgt agtgcaactg ttcggtgtag gagcgttatg gattgcctgc gtgattggct
    14821 atctggcctg ctttgccagt ctcaacattt atatacagag cttcgcccgc ctggtctggt
    14881 cgcaggcgca acataatcct gaccactacc tggcacgcct ctcttctcgc catatcccga
    14941 ataatgccct caatgcggtg ctcggctgct gtgtggtgag cactttggtg attcatgctt
    15001 tagagatcaa tctggacgct cttattattt atgccaatgg catctttatt atgatttatc
    15061 tgttatgcat gctggcaggc tgtaaattat tgcaaggacg ttatcgacta ctggcggtgg
    15121 ttggcgggct gttatgcgtt ctgttactgg caatggtcgg ctggaaaagt ctctatgcgc
    15181 tgatcgtgct ggcggggtta tggctgttgc tgccaaaacg aaaaacgccg gaaaatggca
    15241 taatcacata atccggcgtt tcgacattaa tcctggcgat cgtctttatg atcaaggcgg
    15301 tcgcgctcat catcctttcg ctggtactca ccatcaaaag tattaccgcc accggtcccg
    15361 gcgctaaaac cgccgcctgg catacgagaa aagcgcaaat gcggcatcaa cttcactgtc
    15421 agatgctttt gcaccggcgg taataaaagc agaagaccga ggaagtcggt gaaaaagccc
    15481 ggtaataaaa gcagcaaacc ggcaatgatc agcgaaacac ttttaatcat ctccgccgct
    15541 gggttttcac ccgccgccat tttttgctgc atcagcacga aattcttaaa gccctggtta
    15601 cgtaccagtg acatcccgat aactgacgtg aatataacca gcacgagggt cagcaatacc
    15661 cccaatacat gggcaacctg aataaagatt gaaatctcaa tatagacata aaggaaaatg
    15721 gcaataaaag gtaaccagcg caaaggtttc tcctgtaata gcagccggta aaacccggct
    15781 acctgaatgg gttgcgaatc gcgttcagct tatattgtgg tcattagcaa aatttcaaga
    15841 tgtttgcgca actatttttg gtagtaatcc caaagcggtg atctatttca caaattaata
    15901 attaaggggt aaaaaccgac acttaaagtg atccagatta cggtagaaat cctcaagcag
    15961 catatgatct cgggtattcg gtcgatgcag gggataatcg tcggtcgaaa aacattcgaa
    16021 accacatata ttctgtgtgt ttaaagcaaa tcattggcag cttgaaaaag aaggttcaca
    16081 tgtcaaacaa cattcgtatc gaagaagatc tgttgggtac cagggaagtt ccagctgatg
    16141 cctactatgg tgttcacact ctgagagcga ttgaaaactt ctatatcagc aacaacaaaa
    16201 tcagtgatat tcctgaattt gttcgcggta tggtaatggt taaaaaagcc gcagctatgg
    16261 caaacaaaga gctgcaaacc attcctaaaa gtgtagcgaa tgccatcatt gccgcatgtg
    16321 atgaagtcct gaacaacgga aaatgcatgg atcagttccc ggtagacgtc taccagggcg
    16381 gcgcaggtac ttccgtaaac atgaacacca acgaagtgct ggccaatatc ggtctggaac
    16441 tgatgggtca ccagaaaggt gaatatcagt acctgaaccc gaacgaccat gttaacaaat
    16501 gtcagtccac taacgacgcc tacccgaccg gtttccgtat cgcagtttac tcttccctga
    16561 ttaagctggt agatgcgatt aaccaactgc gtgaaggctt tgaacgtaaa gctgtcgaat
    16621 tccaggacat cctgaaaatg ggtcgtaccc agctgcagga cgcagtaccg atgaccctcg
    16681 gtcaggaatt ccgcgctttc agcatcctgc tgaaagaaga agtgaaaaat atccaacgta
    16741 ccgctgaact gctgctggaa gttaaccttg gcgcaacagc aatcggtact ggtctgaaca
    16801 cgccgaaaga gtactctccg ctggcagtga aaaaactggc tgaagtcact ggcttcccat
    16861 gcgtaccggc tgaagacctg atcgaagcga cctctgactg cggcgcttat gttatggttc
    16921 acggcgcgct gaaacgcctg gctgtgaaga tgtccaaaat ctgtaacgac ctgcgcttgc
    16981 tctcttctgg cccacgtgcc ggcctgaacg agatcaacct gccggaactg caggcgggct
    17041 cttccatcat gccagctaaa gtaaacccgg ttgttccgga agtggttaac caggtatgct
    17101 tcaaagtcat cggtaacgac accactgtta ccatggcagc agaagcaggt cagctgcagt
    17161 tgaacgttat ggagccggtc attggccagg ccatgttcga atccgttcac attctgacca
    17221 acgcttgcta caacctgctg gaaaaatgca ttaacggcat cactgctaac aaagaagtgt
    17281 gcgaaggtta cgtttacaac tctatcggta tcgttactta cctgaacccg ttcatcggtc
    17341 accacaacgg tgacatcgtg ggtaaaatct gtgccgaaac cggtaagagt gtacgtgaag
    17401 tcgttctgga acgcggtctg ttgactgaag cggaacttga cgatattttc tccgtacaga
    17461 atctgatgca cccggcttac aaagcaaaac gctatactga tgaaagcgaa cagtaatcgt
    17521 acagggtagt acaaataaag aaggcacgtc agatgacgtg ccttttttct tgtgaacagt
    17581 aacttaaaaa caacaaccta atatcaactt gttaaaaaac aaggaaggct aatatgctag
    17641 ttgtagaact catcatagtt ttgctggcga tcttcttggg cgccagattg gggggaatag
    17701 gtattggttt tgcaggcgga ttgggggtgc tggttcttgc cgctattggc gttaaacccg
    17761 gtaacatccc gttcgatgtc atttccatta tcatggcggt tatcgccgct atttctgcca
    17821 tgcaggttgc tggcggtctg gactatctgg ttcatcagac agaaaagctg ctgcgccgta
    17881 acccgaaata catcacgatc ctcgcaccta tcgtgaccta tttcctgaca atctttgctg
    17941 gtactggcaa catctctctg gcgacactgc cagttatcgc tgaagttgcg aaggaacaag
    18001 gcgttaaacc ttgccgtccg ctctctactg cagtggtatc cgcgcagatt gcgatcaccg
    18061 catcgccaat ctcagcggca gtggtttaca tgtcttccgt gatggaaggt catggcatca
    18121 gctacctcca tctgctctcc gtggtcatcc cgtccaccct gctggcggtt ctggtgatgt
    18181 cctttctggt cactatgctg ttcaactcca aactctctga cgatccgatt tatcgcaagc
    18241 gtctggaaga gggcctggtt gaactgcgcg gtgaaaagca gattgaaatc aaatccggtg
    18301 caaaaacgtc cgtctggctg ttcctgctgg gcgtagttgg cgtggttatc tatgcaatca
    18361 tcaacagccc aagcatgggt ctggttgaaa aaccgctgat gaacaccacc aacgcaatcc
    18421 tgatcatcat gctcagcgtt gcaactctga ccaccgttat ctgtaaagtc gataccgaca
    18481 acatcctcaa ctccagcacc ttcaaagcag gtatgagcgc ctgtatttgt atcctgggtg
    18541 ttgcgtggct gggcgatact ttcgtttcca acaacatcga ctggatcaaa gataccgctg
    18601 gtgaagtgat tcagggtcat ccgtggctgc tggccgtcat cttcttcttt gcttctgctc
    18661 tgctgtactc tcaggctgca accgcaaaag cactgatgcc gatggctctg gcactgaacg
    18721 tttcaccgct gaccgctgtt gcttctttcg ctgcggtgtc tggtctgttc attctgccga
    18781 cctacccgac gctggttgct gcggtacaga tggatgacac gggtactacc cgtatcggta
    18841 aattcgtctt caaccatccg ttcttcatcc cgggtactct gggtgttgcc ctggccgttt
    18901 gcttcggctt cgtgctgggt agcttcatgc tgtaatgatc aatcgcgggg cgttcacgcc
    18961 ccgctttctt tcccgccgac taacatcctt cccccgtacg ttgtatagtg acctctctct
    19021 tgcggttcca tctgttcttg cgaggtgttt atgcttgatg aaaaaagttc gaataccgcg
    19081 tctgtcgtgg tgctatgtac ggcaccagat gaagcgacag cccaggattt agctgccaaa
    19141 gtgctggcgg aaaaactggc ggcctgcgcg accttgatcc ccggcgctac ctctctctat
    19201 tactgggaag gtaagctgga gcaagaatac gaagtgcaga tgattttaaa aactaccgta
    19261 tctcaccagc aggcactgct ggaatgcctg aagtctcatc atccatatca aaccccggaa
    19321 cttctggttt tacctgttac acacggagac acagattacc tctcatggct caacgcatct
    19381 ttacgctgat cctgctactt tgcagcactt ccgtttttgc cggattattc gacgcgccgg
    19441 gacgttcaca atttgtcccc gcggatcaag cctttacttt tgattttcag caaaaccaac
    19501 atgaccttaa tctgacctgg cagatcaaag acggttacta cctctaccgt aaacagatcc
    19561 gcattacgcc ggaacacgcg aaaattgccg acgtgcagct gccgcaaggc gtctggcatg
    19621 aagatgagtt ttacggcaaa agcgagattt accgcgatcg gctgacgctt cccgtaacca
    19681 tcaaccaggc gagtgcggga gcgacgttaa ctgtcaccta ccagggctgt gctgatgccg
    19741 gtttctgtta tccgccagaa accaaaaccg ttccgttaag cgaagtggtc gccaacaacg
    19801 cagcgtcaca gcctgtgtct gtttcgcagc aagagcagca caccgcgcaa ttgccctttt
    19861 ccgcgctctg ggcgttgttg atcggtattg gtatcgcctt tacgccatgc gtgctgccaa
    19921 tgtacccact gatttctggc atcgtgctgg gtggtaaaca gcggctctcc actgccagag
    19981 cattgttgct gacctttatt tatgtgcagg ggatggcact gacttacacg gcgctgggtc
    20041 tggtagttgc cgccgcaggg ttacagttcc aggcggcgct acagcaccca tacgtgctca
    20101 ttggcctcgc catcgtcttt accttgctgg cgatgtcaat gtttggcttg tttactctac
    20161 aactcccctc ttcgctgcaa acacgtctca cgttgatgag caatcgccaa cagggcggct
    20221 cacctggcgg tgtgtttgtt atgggggcga ttgccggact tatctgttca ccatgcacca
    20281 ccgcaccgct tagcgcgatt ctgctgtata tcgcccaaag cgggaacatg tggctgggcg
    20341 gcggcacgct ttatctctat gcgttgggca tgggcctgcc gctgatgcta attaccgtct
    20401 ttggtaaccg cttgctgccg aaaagcggcc cgtggatgga acaagtcaaa accgcgtttg
    20461 gttttgtgat cctcgcactg ccggtcttcc tgctggagcg agtgattggt gatgtatggg
    20521 gattacgctt gtggtcggcg ctgggtgtcg cattctttgg ctgggccttt atcaccagcc
    20581 tacaggctaa acgcggttgg atgcgcgtgg tgcaaataat cctgctggca gcggcattgg
    20641 ttagcgtgcg cccacttcag gattgggcat ttggtgcgac gcataccgcg caaactcaga
    20701 cgcatctcaa ctttacacaa atcaaaacgg tagatgagtt aaatcatgcg ctcgttgaag
    20761 ccaaaggcaa accggtgatg ttagatcttt atgccgactg gtgcgtcgcc tgtaaagagt
    20821 ttgagaaata caccttcagc gacccgcagg tgcaaaaagc gttagcagac acggtcttac
    20881 ttcaggccaa catcacggcc aacgacgcac aagatgtggc gctgttaaag catcttaatg
    20941 tccttggcct accgacaatt ctcttttttg acggacaagg ccaggagcat ccacaagcac
    21001 gcgtcacggg ctttatggat gctgaaacct tcagcgcaca tttgcgcgat cgccaaccgt
    21061 gaacgacact tgcagcggga aagacggagg aaaatagcgt gcaacgtgaa gatgtactgg
    21121 gagaagccct gaaattatta gaattacaag ggattgccaa caccacgctg gagatggttg
    21181 ctgaacgtgt ggattatcca ctggacgagc tacgccgctt ctggccagac aaagaggcaa
    21241 tcctctacga tgcgctgcgt tatcttagcc aacagataga tgtctggcgt cgtcagctga
    21301 tgctggacga aacgcaaacc gccgaacaaa agcagctggc acgttatcag gcgttatcgg
    21361 agtgcgttaa aaacaaccgc tatccgggct gtctatttat cgctgcctgt acgttttatc
    21421 ccgatcctgg ccaccctatt catcaactgg ccgatcagca aaaaagcgcg gcctacgatt
    21481 tcacccacga actgttaacc acactggaag ttgacgatcc ggcgatggta gcaaagcaga
    21541 tggaactggt gctggaaggc tgtttaagcc gaatgctggt gaatcgtagc caggcggatg
    21601 tcgacactgc acatcggctg gcggaagata tcctgcgctt cgcccgctgc cgccagggtg
    21661 gtgcattgac ctgacagaaa cacagaaaag aagcgatttg ccgcaatctt aagcagttga
    21721 atcgctttta ctgaaattag gttgacgaga tgtgcagatt acggtttaat gcgccccgtt
    21781 gcccggatag ctcagtcggt agagcagggg attgaaaatc cccgtgtcct tggttcgatt
    21841 ccgagtccgg gcaccaaatt catatcaacg gacctctacg gaggttcgtt tttcgtttca
    21901 gaacgccacg atttaagcgt tctgcctaca aatcaattct accgaactca accagattcc
    21961 ccccacatca accccattgt gtgggtataa ttgcgggcat accccagttc gacagaattt
    22021 gtaccctctt taacgcattc agaaggctga actatggcac tgactgacgc aaaaatccgg
    22081 gctgcaaagc ccactgacaa ggcttataaa ctcactgacg ggactggcat gttcctgctg
    22141 gtacatccta atggctcccg ttactggcgt ctccgttatc gtattctggg taaggagaag
    22201 actctggcac ttggtgtgta tccagaagtt tctctctccg aagctcgtac aaaacgggat
    22261 gaggcccgaa aactgatttc ggagggggtt gacccttgcg aacagaaaag agctaaaaaa
    22321 gtagtccctg atttacagct ctcttttgaa catattgcgc gacgctggca tgccagtaat
    22381 aaacaatggg cacaatcaca cagcgataaa gtactcaaaa gcctcgagac tcacgttttc
    22441 ccctttatcg gcaaccggga tatcacaaca ctcaataccc cggatctgct tatccctgtt
    22501 cgtgctgcag aagctaaaca aatttatgaa atcgccagtc gtctgcagca aagaatatct
    22561 gccgtaatgc gttatgccgt acagtctggc atcatcagat ataatcctgc tctggatatg
    22621 gctggcgcat tgactacggt aaaacgccag catcgccccg ctcttgatct ttctcgcctg
    22681 cctgaacttt tgtcgcgtat tggcagttac aaagggcaaa ctgtcaccca gcttgccgtt
    22741 atgctgaatt tactggtttt tattcgttcc agtgaactca gatacgcccg ctggtctgaa
    22801 attgatattg acaatgccat gtggactatt ccagccgaac gcgaacccct gccaggcgta
    22861 aaattctcac accggggctc caagatgcga acaccacatc ttgtgccact cagcaaacag
    22921 gctgtagcca tactgacaga actacagaca tgggctggtg aaaatggtct gatctttacg
    22981 ggagcacatg acccgcgtaa accaatcagt gaaaatgtaa tgaacgcatc
//
LOCUS       DADLFA010000050        22931 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000050 DADLFA010000000
VERSION     DADLFA010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22931
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.050"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..745)
                     /locus_tag="JQH65_001656"
     CDS             complement(<1..745)
                     /locus_tag="JQH65_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY6139109.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSVGAVLLAAALVGTSSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLTAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISS"
     gene            963..2615
                     /locus_tag="JQH65_001657"
     CDS             963..2615
                     /locus_tag="JQH65_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HAY6139110.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDVEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQAPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVTKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(2652..3131)
                     /gene="ybaK"
                     /locus_tag="JQH65_001658"
     CDS             complement(2652..3131)
                     /gene="ybaK"
                     /locus_tag="JQH65_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HAY6139111.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMAAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(3335..4129)
                     /locus_tag="JQH65_001659"
     CDS             complement(3335..4129)
                     /locus_tag="JQH65_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6139112.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMTPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQITMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            4267..4608
                     /locus_tag="JQH65_001660"
     CDS             4267..4608
                     /locus_tag="JQH65_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HAY6139113.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSTL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(4823..7327)
                     /gene="copA"
                     /locus_tag="JQH65_001661"
     CDS             complement(4823..7327)
                     /gene="copA"
                     /locus_tag="JQH65_001661"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HAY6139114.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARVVTAEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALI
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNPALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKVVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            7589..8521
                     /gene="glsA"
                     /locus_tag="JQH65_001662"
     CDS             7589..8521
                     /gene="glsA"
                     /locus_tag="JQH65_001662"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAY6139115.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAVVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENIEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGLN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            8524..9816
                     /locus_tag="JQH65_001663"
     CDS             8524..9816
                     /locus_tag="JQH65_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY6139116.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVAHAFGAYAVQFLHEGSQEEHLILLYALGSIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGDV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            9941..10348
                     /locus_tag="JQH65_001664"
     CDS             9941..10348
                     /locus_tag="JQH65_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CueR"
                     /protein_id="HAY6139117.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(10349..10807)
                     /locus_tag="JQH65_001665"
     CDS             complement(10349..10807)
                     /locus_tag="JQH65_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HAY6139118.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(10804..11721)
                     /locus_tag="JQH65_001666"
     CDS             complement(10804..11721)
                     /locus_tag="JQH65_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HAY6139119.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            11867..12544
                     /gene="fetA"
                     /locus_tag="JQH65_001667"
     CDS             11867..12544
                     /gene="fetA"
                     /locus_tag="JQH65_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HAY6139120.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNRQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            12531..13310
                     /gene="fetB"
                     /locus_tag="JQH65_001668"
     CDS             12531..13310
                     /gene="fetB"
                     /locus_tag="JQH65_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HAY6139121.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDIFWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(13373..14227)
                     /gene="cnoX"
                     /locus_tag="JQH65_001669"
     CDS             complement(13373..14227)
                     /gene="cnoX"
                     /locus_tag="JQH65_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HAY6139122.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(14288..15097)
                     /gene="ybbO"
                     /locus_tag="JQH65_001670"
     CDS             complement(14288..15097)
                     /gene="ybbO"
                     /locus_tag="JQH65_001670"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HAY6139123.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNNMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(15087..15710)
                     /gene="tesA"
                     /locus_tag="JQH65_001671"
     CDS             complement(15087..15710)
                     /gene="tesA"
                     /locus_tag="JQH65_001671"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HAY6139124.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            15681..16367
                     /locus_tag="JQH65_001672"
     CDS             15681..16367
                     /locus_tag="JQH65_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY6139125.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            16364..18376
                     /locus_tag="JQH65_001673"
     CDS             16364..18376
                     /locus_tag="JQH65_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY6139126.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSMVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMQLNVLCRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRCDLLDRWQQLPPESPNYFLINIATEQVAPLKAFL
                     AEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNWP
                     PKADEVSMEEGLAKRLNVALGGTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFPE
                     GALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVG"
     gene            18377..18775
                     /locus_tag="JQH65_001674"
     CDS             18377..18775
                     /locus_tag="JQH65_001674"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAY6139127.1"
                     /translation="MLEQVSRALEVMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRT
                     LGAGKKLLRTTLWCEFAMLGFVSGLVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLP
                     CSGALLLSLFGGWLGARLVKGKALFRQFAG"
     gene            19204..22931
                     /locus_tag="JQH65_001675"
                     /pseudo
     CDS             19204..22931
                     /locus_tag="JQH65_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT         5288 a         6004 c         6484 g         5155 t
ORIGIN      
        1 agctgctgat gctcgccata tagaaatatt tcgagaaaat gaggattaac agaatgctga
       61 ctgccagtac cactttgttg cgcggcagtg gattgattat cgtcgctttg ggttttcctt
      121 tattcattcg gtgctgtgcc gagtaccaac ggctaatttg cgccaacacc acgatcgcca
      181 gcagtgccgc aagcacaaac caggcaacgt tgcctttgcc ataaggcgcg ataatcaccg
      241 ccgtcagcaa gggtcccagg gaactgccaa agttgccgcc gacctgaaag atagattgcg
      301 ccaggccatg ccgcccgccg gaagccatac gggctacgcg agaagattcc ggatgaaaga
      361 ccgatgaact ggtaccgacc agcgccgccg ccagcagaac tgcgccaaca ctgcccgcca
      421 gcgcaagcag caccagacca cttaaggtaa agcacatgcc aattggcaac gaccacggca
      481 tcggatattt atcggtccag tagccgacca ctggttgcag tagcgaagag gcgagctgga
      541 aggtgagggt tatcatgcca atctgcataa atgtcagaga aaattctgac tgaagcagag
      601 gataaatcgc cagaatcagc gattggatca tgtcgttcag cagatgtgag aggctgatag
      661 cacctaaaat accaaacgat gttcgggcct tggtcgttga cgcagccgcg cccgccacag
      721 gctgggtttg ttcactcatt gccataggaa agtcactttt tcagggttgc gatgtaaaga
      781 atgatcttat ttgtgattat tacccgacta acatacctgt aagcgtcgcc tgaaggaagt
      841 ctcaacgccg aatacagaat ttctaatctg gatgcagatt tatcttcacc ggacgcagac
      901 ttgtctatta tgttgcgtca tactattttt caacacgttg aaatcaggtc agggagagaa
      961 gtatgaaatt attgcagcgg ggcgtggcgt tagcgctgtt aaccacattt acactggcga
     1021 gtgaaactgc tctggcgtat gagcaggata aaacctacaa aattacagtt ctgcatacca
     1081 atgatcatca tgggcatttt tggcgcaatg aatatggcga atatggtctg gcggcgcaaa
     1141 aaacgctggt ggatggtatc cgcaaagagg ttgcggctga aggcggtagc gtgctgctac
     1201 tttccggtgg cgacattaac actggcgtgc ccgagtctga cttacaggat gccgaacctg
     1261 attttcgcgg tatgaatctg gtgggctatg acgcgatggc gatcggtaat catgaatttg
     1321 ataatccgct caccgtatta cgccagcagg aaaagtgggc caagttcccg ttgctttccg
     1381 cgaatatcta ccagaaaagt accggcgagc gcctgtttaa accatgggcg ctgtttaagc
     1441 gtcaggatct gaaaattgcc gtcattgggc tgacaaccga tgacacagca aaaattggta
     1501 acccggaata cttcactgat gtcgaatttc gtaagcccgc cgatgaagcg aagctggtga
     1561 ttcaggagct gcaacagaca gaaaagccag acattattat cgcggcgacc catatggggc
     1621 attacgataa tggtgagcac ggctctaacg caccgggcga tgtggagatg gcacgcgcgc
     1681 tgcctgccgg atcgctggcg atgatcgtcg gtggtcactc gcaagctccg gtctgcatgg
     1741 cggcagaaaa caaaaaacag gtcgattacg tgccgggtac gccatgcaaa ccagatcaac
     1801 aaaacggcat ctggattgtg caggcgcatg agtggggcaa atacgtggga cgggctgatt
     1861 ttgagtttcg taatggcgaa atgaaaatgg ttaactacca gctgattccg gtgaacctga
     1921 agaagaaagt gacctgggaa gacgggaaaa gcgagcgcgt gctttacact cctgaaatcg
     1981 ctgaaaacca gcaaatgatc tcgctgttat cgccgttcca gaacaaaggc aaagcgcagt
     2041 tggaagtgaa aataggcgaa accaatggtc gtctggaagg cgatcgtgac aaagtgcgtt
     2101 ttgtacagac caatatgggg cggttgattc tggcagccca aatggatcgc actggtgccg
     2161 actttgcggt gatgagtgga ggaggaattc gtgattctat cgaagcaggc gatatcagct
     2221 ataaaaacgt actgaaagtg cagccattcg gcaatgtggt ggtgtatgcc gacatgaccg
     2281 gtaaagaggt gattgattac ctgaccgccg tcgcacaaat gaagccagat tcaggtgcct
     2341 acccgcaatt tgccaacgtt agctttgtga cgaaagacgg caaactcaac gacctgaaaa
     2401 tcaaaggcga accggtcgat ccggcgaaaa cttaccgtat ggcgacatta aacttcaatg
     2461 ccaccggcgg tgatggctat ccgcgccttg ataacaaacc gggctatgtg aataccggct
     2521 ttattgatgc cgaagtgctg aaagcgtata tccagaaaag ctcgccgctg gatgtgagtg
     2581 tttatgaacc gaaaggtgag gtgagctggc agtaatccga aagtgccgga tatttgcatc
     2641 cggcacaatg cttaatcgcg gcgggcgata tcagcaaatt tggcatcgag gatcttcgcc
     2701 agatcgcctg ccgccagttc gatatccagt ccgcgcttgc cgccagaaac ataaatagtg
     2761 gcaaattctt gtgcgggggc gtcgataatc gtcggcagac gttttttctg ccccagtggg
     2821 ctaatccccc caaccaggta tcccgtcgaa cgctgcgcgg ccatcggatc ggccatctca
     2881 actttcttgg cacccagcgc ttttgctact tttttaagat ccagttgacc tgcgaccggc
     2941 gtaacggcca ctgccagatg tttcatatca ccgttcactg ccaccagtag cgttttgtag
     3001 acttgatccg gattcaaacc taattttttg acgacttcat cgccaaaatt ggtttcagcc
     3061 ggatcgtgct cgtaggtatg gatttgaaac gaaatcttgt ttttttcgag taatttaact
     3121 gcgggtgtca tggtaatttt cctgccttaa acaaaaaaac gaagcaagca tacgcctgat
     3181 ggcggtccaa aaaaaagagt catcttgcct aagagtattg gcaggatggt gagattgagc
     3241 gacaatcgag ttacaccgtc gcttaaagtg acggcataat aataaaaaaa tgaaattcct
     3301 ctttgacggg ccaatagcga tattggccat ttttttagcg caacatttgc ggcaaattcc
     3361 cttctccata caggtgtagt gcaccgaccg cgaccacata tcgccccggc ggcatggcgc
     3421 gtaatttatc ccgccaggcg agatttcgct gatgcatcag cacatcgtac agcgactgac
     3481 tgaacgtatt gggcagcgtt atatcattat tttgcggagg tgcattcagc caccagctca
     3541 tcatttgttg cagcaaccgt gcgttggtat gccagtgggt cagcgtatcg tccagcagcg
     3601 ccagtccttt gtcagggagc tggagcaaca tggtaatctg gttttcagcc ccttccagtt
     3661 caatcacggg tttatgttgt tgcttcgccg cctgcaataa ctggtaatcg ataccgtatt
     3721 ctgcccgcag ccccagtttt tgtgcctgcg tcgcctgaag aaccatcgcg atttgccaca
     3781 gcggttgagt agaaaagagc gacggagaaa tgcccatctc ctggctaatg tgctgcagat
     3841 tttggagttg ctcctcgtta atgcgctctt ccagcgcctc gcaagtaggc aaattagcaa
     3901 aaggcgtatc gctggtggaa acatccgcct cgacgatcag cgcatcggcg tttttgagct
     3961 ttttgagcaa acgggtgggc aggggagtca tatcgtggct acccatatga atactgccaa
     4021 tcagatgaaa atggcgattg ccgggaaggg tgatatcgat ggcaggccag gtgtaatgat
     4081 taccgcgcag cgcggcccaa agcgttttta cccggtacaa cagatccata cgacctccct
     4141 ttgtgaaata tcatgctagc gcgcggtgag ggatggcgca aatggagttg gggacttgtt
     4201 taatgtgttt gtatgattca gtatgttctt gcatcgctat tcacagggaa gcaacagtta
     4261 aaaaccatga aacaggcaac aagaaaaccg acgacacccg gagatattct tctctatgaa
     4321 tatctggaac cgctcgattt gaaaatcaat gagttagcag agttgctgca tgttcatcgt
     4381 aatagcgtca gtacactgat caataataat cgtaaactca ccactgagat ggcattccgt
     4441 ctggcgaaag tttttgatac cacagtcgat ttttggctaa acctccaggc ggcagttgat
     4501 ctttgggaag ttgaaaacaa catgcgcacc caggaagaat tgggacggat tgaaacagtg
     4561 gctgaatatt tggcacgccg tgaagagcgt gcaaaaaagg tcgcgtaagg ccaaaacatc
     4621 gcttcatctc gtaggccgga taaggcgttc acgccgcatc cggcatccga gcaccattgc
     4681 ctgatgcgac gcttacccgt cttatcaggc ctacaaacct gtgcctgaac cgtaggccgg
     4741 ataaggcgtt tacgccgcat ccggcagtca tgcgtcgatg ccaaatgcgc cgccctaaag
     4801 cagcgcatcc gcaatgacgt acttattcct tcggtttaaa ccgcagcaac cggttggcgt
     4861 tactcactac ggtaatcgac gagagcgcca ttgccgctcc ggcaactacc gggttaagca
     4921 gtgttccagt aaacggccac aaaataccag cggcgaccgg aataccaatg ctgttgtaga
     4981 taaacgcacc gagcaggttc tgcttcatat tgcgcagcgt tgcgcgggaa atcgcgagcg
     5041 catctgcaac acccatcagg ctatggcgca tcagggtaat cgccgcggtt tcaatggcaa
     5101 catcactgcc gccacccatc gcaataccga catccgcctg agccagcgct ggcgcgtcgt
     5161 taatgccgtc gcccaccatt gccacctgac gtccttcact ttgcagacgt ttgatcgctt
     5221 cggctttacc gtctggcagc accccggcga ttacctcatc aatccctgct tctttggcga
     5281 tcgcattggc ggtggttggg ttatccccgg tcaacatcac cagacgatat cccgctttat
     5341 gcaggcgttg cagcgccgcc acgctatcac tacgcaacgg atcgcgtaca gccagcaggg
     5401 ctactacttt cccgtcaatc gccagcagca caggcgttgc tccttgcgat gcctgagctg
     5461 aaatatcggc ttcaatcgct ttagtatcga cttgctggtc atttaacagc gccggattac
     5521 ccagcaataa cgcatgacct tcagcttcac cgcttacgcc cagcccgcgc aatgtgcgga
     5581 aaccgttgac ctgcggcagc tgcatatcgc ctgctttatc gaggatcgct cgtgccagcg
     5641 gatggctgga accttgctcc agcgccgccg ccagacgcaa tgcttgcgct tcatcaacat
     5701 cagcaaatgt ttttactgcg acaacctgcg gcttcccttc ggtcagcgtc ccggttttat
     5761 cgaacacgac ggtatcgagt gtactggcgc gttgcagcgc gtcggcgtcc cgcaccagca
     5821 cgccaaactc agccgcccgc ccgacgccgg aaataatcga cattggcgtc gccagcccca
     5881 gcgcacacgg acaggcaata atcagcaccg tggtggctat caccagggta tagacaatct
     5941 gcggtgccgg accaaagaaa taccagattg ctgcactgat cagcgcaatc accactacta
     6001 ccggcacaaa tacggctgag attttatccg ccagctgacc gatttctggc ttgctgctct
     6061 gggcctggcg caccatgcga ataatccttg acagcgtggt atggctgcca accgcactgg
     6121 cgcgaaatag cacgctaccg tcctgtacca ctgtcccggc atggacgctt tcgccttcgc
     6181 ctttttgctg cgggattggt tcgcccgtca gcatcgcttc atccagccat gcttcgccct
     6241 gggtaatctc gccatctacc ggcacgcgat cgccggtcgt caggcgcagt aacatccctg
     6301 gctgaacttc cgccagcggc acgctttttt caccctctgc ggtgaccact cgcgccgtcg
     6361 gtggggttaa atcgagtaat ttctccagcg ccttagaaga acgctgacgc gcgcgcgctt
     6421 ccagcatatg gccgagatta atcagaccga taatcatcgc actggcttca tagtaaagat
     6481 gccgcgcttc catcgggaac cactgcggcc acaggttgac gctcatcgaa tagagccacg
     6541 ccacacccgt acccagcgcc accagcgtat ccatcgtcgc cgcaccgttc agcaggcttt
     6601 tccacgcgct gcggtaaaaa tgcccgccgg cgaaaaccat taccgccagg gttatcaggc
     6661 cgataactaa ccacaggctg cggttgtcag cggtgaccat catgttatcg ccgatcatcc
     6721 cccagaccat taccggaata cccaccgcca gtgcgacaat cgcctgccag cggaagcgct
     6781 tcatcgtagc gacggcggtt tcctgctggc gctcgcggcg tttcgcgtca tcttcaatcg
     6841 cttccgcgcc gtagcccgct ttttccaccg cctgcactaa atcttgtggg gaggcactgc
     6901 ccatcaccag cgcagtacgc tccgccaggt ttacccgtgc ctgagtgacg cccggtacgc
     6961 tttgcagcgc attttgtacg cgggtgacac agctggcgca gctcatgccg ctcagcagca
     7021 actgctggct gtcatcgtca tcggcggtcg ctgccggaag cgcctcagaa accgctgtca
     7081 gtgcttccga cgggattgat gactccgcca gcggtttagc ctttgggtgg cttacagatg
     7141 cgtcataacc cgcttgtttg atggtttcga tcagctgttc tgcactggca gtcccggtaa
     7201 cgtgcgcttc agtgatagac acatccgctt gttccacatc cgggcgctgt tcgagacttt
     7261 ctttcacgcg tttaacgcag tgaccgcagg acaggccgtc cagggtcagg tcgatagttt
     7321 gtgacataaa acactccttt aagacagttt tgactggctg tgataaaggt taaaccttcc
     7381 agcaagggga aggtcaagaa attaataaac caggcgggta aaagtccgta aagattaaaa
     7441 aatcggctcg atttgcatca ggattagaca tttatttctt tgttttcctg tagttaagtt
     7501 gctggtgcta agttaaatca ggatgcctga aaatcggcac cggggtgagg aattacctcc
     7561 cgcatctata aaaaggagtt aacaaaagat gttagatgca aacaaattac agcaggcagt
     7621 ggatcaggct tacacccaat ttcactcact taacggcgga caaaatgccg attacattcc
     7681 ctttctggcg aatgtaccag gtcaactggc ggtagtggct atcgtgacct gcgatggcaa
     7741 cgtctatagt gcgggtgaca gtgattaccg ctttgcactg gaatccatct cgaaagtctg
     7801 tacgttagcc cttgcgttag aagatgtcgg cccgcaggcg gtacaggaca aaattggcgc
     7861 tgacccgacc ggattgccct ttaactcagt tatcgcctta gagttgcatg gcggcaaacc
     7921 gctttcgcca ctggtaaatg ctggcgctat tgccaccacc agcctgatta acgctgaaaa
     7981 tattgaacaa cgctggcagc gaattttaca tatccaacag caactggctg gtgagcaggt
     8041 agcgctctct gacgaagtca accagtcgga acaaacaacc aacttccata accgggccat
     8101 agcctggctg ctgtactccg ccggatatct ctattgtgat gcaatggaag cctgtgacgt
     8161 gtacacccgt cagtgctcca cgctcatcaa tactgttgaa ctggcaacgc ttggcgcgac
     8221 gctggcggcg ggtggtctga atccgctgac gcataaacgc gttcttcagg ccgacaacgt
     8281 gccgtacatt ctggccgaaa tgatgatgga agggctgtat ggtcgctccg gtgactgggc
     8341 gtatcgcgtt ggtttaccgg gcaaaagcgg tgtaggtggc ggtattctgg cggtcgtccc
     8401 tggcgtgatg ggaattgccg cgttctcacc accgctggac gaagaaggta acagtgttcg
     8461 cggtcaaaaa atggtggcat cggtcgctaa gcaactcggc tataacgtgt ttaagggctg
     8521 atcatgatga acacggaagg taataacggt aacaaacccc tcggtctatg gaatgtcgtt
     8581 tccatcggta ttggggcaat ggtgggggcg gggatcttcg cgctgctggg gcaggctgcg
     8641 ttgctaatgg aagcctcgac ctgggtcgcc tttgcttttg gcggtattgt ggcgatgttt
     8701 tccggttatg cctatgcgcg tctgggggcg agctatccca gcaatggcgg cattatcgac
     8761 ttctttcgtc gcggattagg caacggcgtc ttttcgctgg cgctctcgtt actgtacctg
     8821 ttgacgctgg cggtgagcat cgccatggtc gcccatgctt ttggcgctta tgccgtgcag
     8881 tttttgcatg aaggcagcca ggaggagcac cttattttgc tctacgcgtt ggggagcatt
     8941 gcggtgatga cgcttttcaa ctccttaagc aaccatgcgg tagggcggct ggaagtgatc
     9001 ctcgtcggca ttaaaatgat gatcctgtta ttgctgatta ttgccggtgt ctggtcgctg
     9061 caaccagcgc atatttccgt ctctgcgccc cccagctccg gtgcgttctt ctcctgtatt
     9121 gggataactt tccttgccta tgcgggcttt ggcatgatgg cgaacgcggc ggataaagtg
     9181 aaagatccgc aggtcattat gccacgggcg tttctggtgg cgattggcgt taccacgttg
     9241 ctttatatct cgctggcact ggttttgctt agcgatgtat cggcattaga gttagaaaaa
     9301 tatgccgata ccgccgtagc gcaggctgct tctccgctgc tcggagatgt gggttatgtg
     9361 atcgtcgtca tcggcgcttt actggcgacg gcttcagcca ttaacgcgaa cctgttcgcc
     9421 gtgtttaaca tcatggacaa catgggcagc gaacgcgaac tgccgaagct aatgaataaa
     9481 tccctgtggc ggcagagtac ctggggcaac atcattgtcg tggtgttgat tatgctgatg
     9541 acggcggcac tgaatttagg ctcactcgcc agcgttgcca gcgccacctt tttgatttgc
     9601 tacctggcgg tgtttgtggt ggcgatccgc ctgcgtcatg atattcacgc ctcgttgccg
     9661 attcttatcg ttggtacgtt ggtgatgttg ttggtgatcg ttggctttat ctacagtctg
     9721 tggtcccagg gtagccgtgc gttgatatgg attattggct cactcttact cagccttatt
     9781 gtggcaatgg tcatgaagcg caataaaacc gtataacatc tctctgtgcg cagtacttcc
     9841 tgtattattg tggtggcggt cgatattcgc actggcaaaa aaacgtgctt gaatatctgt
     9901 tgaaaccctt taacaaagca caggaggcgt tgcgcgaacg atgaacatca gcgatgtagc
     9961 aaaaattacc ggcctgacta gcaaagccat tcgcttctat gaagagaagg ggctggtgac
    10021 gccgccgatg cgcagcgaaa acggttatcg cacctacacg cagcagcatc tcaacgaact
    10081 gaccttactg cgccaggcac ggcaggtggg ctttaacctg gaagagagcg gcgagctggt
    10141 gaatctgttt aacgacccgc agcggcacag cgccgacgtc aaacggcgca cgctggagaa
    10201 ggtggcggag atcgaacgac acattgagga gctgcaatcc atgcgcgacc agctgctggc
    10261 actggcgaat gcctgccctg gcgatgacag cgccgactgc ccgattatcg aaaatctctc
    10321 cggctgctgt catcatcggg cagggtgatt aagacgagac ggctcggata tgtagggtta
    10381 tcccttctat cgcaatgact tcaacatgcg tacctgcacc gagatcctcg ctggcgctga
    10441 caggccatga actgtcaccg acgcgcatat gaccgcgccc gttgaccagc ggagattcca
    10501 gcacaaaacg tcggccaatc agctgctgcc cgcgctggtt taaatgacta tcgctgtgct
    10561 tttgttcgcg cacccgccgc gacaaccatt tccaccacag ccaggcggcg agcagcgtca
    10621 ggatggcaaa cattacccct tgccactccc aacccagcgg caccagccag accaccaggc
    10681 cggtaatcac cgctgccacg ccgctccaca acaaataacc atttccgccc agcatctcgg
    10741 ctgccagcag caaaccgccg agactgagcc agaaaatatg tggatgaacg accattaact
    10801 ccatcatggc tgagtccgtt tgttggcgct gtctttcacc agttcggcaa tcccggcaat
    10861 cgaccccatc aggctgctgg cctctaatgg catcatcact actttgctgt tactggagga
    10921 accgatctgc tgtaacgctt cggtgtattt ctgcgctacg aagtagttca ccgcctgaat
    10981 atcaccggag gcgatagctt cagacaccat tttggtggcg cgggcttctg cttcggcgga
    11041 acgttcacgc gcttcagcct gtaaaaacgc cgactgacgt tcgccttccg ctttcaggat
    11101 ttgcgactgt ttttcacctt cggctttgag gatttccgcc tgacggatcc cttccgcttc
    11161 aagaatgtaa gcgcgtttgg tacgttccgc tttcatctgc gcgttcattg aagagataag
    11221 ctctgccggt gggcgcacgt cgcgaatttc aatacgggtg actttaatcc cccacgggtt
    11281 ggtggcttca tcgacaatac gcagcaggcg tgagttgatg ctgtcgcgct gagagagcat
    11341 ttcgtcaagt tccattgaac ccaacacggt acggatgtta gtcatggtca ggttgatgat
    11401 cgccagctcc agattgctga cttcataggc agcgcgtggc gcgtcaatca cctgaataaa
    11461 acagactgcg tcgatggtaa cgttggcgtt atctttcgag ataacttcct gggaagggat
    11521 atcgagcact tgctccatca tattgatctt gcgaccaatg cgatccataa acggcaccac
    11581 cagactgagc cccggctgta acgttttggt atagcgacca aaacgttcta ccgtccattg
    11641 atagccctgc ggcacgattt tgacgcccgc gccgacaatg accagcgcga caaaaatgag
    11701 aatcgggata aagataagca tcggaaaaac ctcctgttgt accgtccata atcagcaaaa
    11761 ttgctgcttg attaaacaaa ttatacctga ttactgaaag agagttcccc cttattcctg
    11821 cgaaggataa actgttttta gtaaaaatca gaaaaaggga acagcgatgc aggaaaatag
    11881 tcctttgctt cagctacaaa acgtaggata tctggcgggt gatgcgaaga ttcttaataa
    11941 catcaatttt tcgctgcgtg ctggcgaatt taagttaatt accggtcctt ctggttgtgg
    12001 caaaagtacg ctgctaaaaa tagttgcttc attgatcagc ccaaccagcg gaacgttact
    12061 gtttgaaggt gaggatgtca gcacactaaa gccagaaatc taccgccaac aagtctctta
    12121 ctgcgcccag acaccgacgc tgtttggcga tacggtatac gataatctga tctttccctg
    12181 gcagatccgt aaccggcagc ctgacccagc catttttctc gattttctcg aacgcttcgc
    12241 cttgccggac agcattttga cgaaaaatat cgccgagcta tctggtggtg aaaaacaacg
    12301 catctcactg attcgtaacc tgcaatttat gccgaaggtt ttattgctgg atgaaatcac
    12361 cagtgcgctg gatgaaagta ataaacataa cgttaatgag atgatccatc gttatgtgcg
    12421 cgagcaaaat attgccgtgc tgtgggtgac acacgataaa gacgaaatta atcatgcgga
    12481 taaagtgatt acactgcaac cgcatgccgg agaaatgcag gaagcacgct atgaactcgc
    12541 ataatattac taacgaatca ttggcactgg cattaatgct ggtggtggtg gcaatcttaa
    12601 ttagccataa agaaaaactg gcgctggaga aagatatttt ctggagcgtc gggcgcgcga
    12661 taattcagct gattattgtc ggctatgtgc tgaagtatat tttcagcgtg gatgatgcca
    12721 gcctgacatt attgatggtg ttatttatct gctttaatgc ggcgtggaac gcgcaaaaac
    12781 gcagtaaata tattgctaaa gcttttatct catcgtttat tgccattacg gttggggcgg
    12841 gaattaccct ggcggtgctg attctctcag ggtcgattga atttatcccg atgcaggtga
    12901 tccctatcgc cgggatgatt gccggtaacg ccatggtagc ggtggggttg tgttacaaca
    12961 atttagggca acgggtcatt agcgaacagc aacagattca ggagaaactg agtcttggcg
    13021 cgacgccgaa gcaggcttca gcgatattga ttcgcgacag tattcgcgcg gctttaattc
    13081 cgacggtcga ttcagcaaaa acggttggct tagtgagttt accaggaatg atgtccgggc
    13141 tgatatttgc cgggattgat ccggtgaagg cgattaaata tcagattatg gtgaccttta
    13201 tgctgctctc aaccgccagc ttgtcgacca ttattgcctg ctatttaacc tatcgtaagt
    13261 tttataattc gcgccaccag ttggtggtga cgcaattgaa gaagaaatga tgcgatgccg
    13321 gatgcgcagc atcgcatccg gcgttgtggt tcatgtgccg aacaacggaa tatcaataca
    13381 acaatgcata cagctggcgg cgatacttcg acgccagtgc atcacccgta cccagcgcag
    13441 cgaggatctc ctggaacgtt ttacgcgtct gaccgtctgc ggcggtgaga tctttacgca
    13501 gatgcccgaa cagcaactcc agcgcctctt cattgcgccc aacctgatgc agttgcagcg
    13561 ccagttgcgt cgccagtgcg gcatcttctg gattctccgc cacctgctgt tgcaactgtt
    13621 gaatttccgg cgtatcagcc gcctgcttca gcagttcgat ttgcgccacc agcccctggt
    13681 agcgggtgtc ctgatcctgc aacggaatgg ttttcagcac cgcttccgca tcttcagaac
    13741 ggttcagcgc aatcagcgtt tctgccagca gcaggccgat ctccccgttc tgattcgaca
    13801 actgccaggc gtctttcagc aatggcaggg catcggtgta attgccttcc tgcatcagtt
    13861 gcatcgcctg ctgcgctttc agctcttctt cgcgcggcag cactttatcc agcagggcgc
    13921 ggatcgcctc ttccggttgc ggcccctgga agccatctac cggttgcccg ttctggaaca
    13981 gatacacggt cggaatcgcg cgcagaccaa actgggcggc aatcatctgc tccgcgtcgc
    14041 agtccagctt cgccagaata aattgcccgt tgtactgcgc cgcgaggctt tccaggatcg
    14101 gggttaactg caaacagtgc tggctacgtt cagaccaaaa atagaacagc accggagtgg
    14161 tcatcgactg ttcaagaacc tgttgcaggt tagattcgtt aatgttgaca atattttcta
    14221 cggacatgga gtcgctctct gttgtcgata ttttctttga catgggggct taagcgcgcg
    14281 cttcaactca cccctgcaat attttgtcca tcacgcgccc aggcagcagg cgcttgagca
    14341 ccattactgc ccaggtcacc agcgttaccg gatagcgcat cttcggcttc tcgctaataa
    14401 aagcatggcg cactttgtcg accaccgctt ccggtcccaa cgtaaagcgg gcggcgatgc
    14461 cgggattttc gactggttta tcactttgcg tctggttgac gttgtcggtg aagcgagtac
    14521 gaatgggacc gggttcgatc aggctgactt taattccgct gtggcgtagc tccatgcgca
    14581 gcgcgtctga ccacgcctcc agcgcatatt tactggccgc gtaagcgcca cgacccggcg
    14641 tggagattaa tcccatcacc gatgatgtca tcacaatacg cccttcaccg tgcggtaaca
    14701 tcgcgggtaa caggcgcatg gtgagctggt gtgcgccgaa aaagttggcg gaaaactgct
    14761 gttccatctg cgcacggctg atggtggaaa gggggccata catgccgaat ccggcattgt
    14821 taaagatccc atacagacaa ttatcggtca gggcgatcac ctcgtctgct gcgcgatcaa
    14881 cactttctgg tgaatccaga tcgatcaaca cgccggtaaa tcccatgttg ttcatacgct
    14941 caacatcatc cggttttcgg caacctgcca gcacatgaaa accctggcgt tttaattcga
    15001 gcgcgctttc caggccaatt ccactggaac atccggtaat taagaccgat ttttgcataa
    15061 ctttacctgt caggatctcc gttgctttat gagtcatgat ttactaaagg ctgcaactgc
    15121 ttcgccatcc agtcggcaat aaacggctgg gcgtcgcggt tgggatgaat accgtcatcc
    15181 tgcatccatt gtggcttgag gtagacctct tccataaaaa agggcagcag cggaacatca
    15241 aactctttgg cgagtttggg gtaaatggcg ctaaaggctt cattataacg gcgaccatag
    15301 tttgcaggca gacgtatttg cattaacaat ggttcagcgt tggcggcttt gacatcctgc
    15361 aaaatctggc gcagcgtttg ctcggtttgc tgtggctgaa aaccacgcaa accgtcattg
    15421 ccgcccagtt caaccagcac ccagcgcggc tgatgctgtt tcagcagagc cggaaggcgc
    15481 gccagtcctt gttgcgaggt gtcgccgctg atgctggcat taactaccga cgttttactc
    15541 tgccacttat cattcaacaa ggcaggccag gccgcgctgg cagacattcg atacccggcg
    15601 ctcaggctat cacccagaat caataacgtg tccgctgcgg cggcacggaa ggttaacagg
    15661 accaggaaca ggaagggcaa atgccagcgg aaaacattgt tgaagttcat catcttaaga
    15721 agtccgtcgg tcagggggag catgaactct ccatcctcac cggagttgag ctggttgtca
    15781 aacgtggcga gaccatcgca ctggtgggcg agtcgggatc gggtaagtca accttgctgg
    15841 cgatcctcgc cgggcttgat gacggcagca gtggcgaagt gagtctggtg ggacaaccgc
    15901 tacataatat ggacgaagaa gcgcgggcaa agttgcgcgc gaagcacgtc ggctttgttt
    15961 ttcagtcatt tatgttaatt cctaccctta acgcgctgga aaacgtcgag cttccggctc
    16021 tgctgcgcgg tgagagtagc gcggaaagtc gtaacggggc gaaagcgttg ctcgaacagt
    16081 tagggctggg taaacgtctg gatcatcttc cggcacagct ttccggcggt gaacagcaac
    16141 gagtggcgct ggcacgagcc tttaatggtc gacctgatgt gctgtttgcc gacgaaccca
    16201 ccggcaacct tgaccgccag acgggcgata aaattgccga cctgctgttt tccctcaacc
    16261 gtgaacatgg caccacgttg attatggtga cccacgacct gcaactggcg gcacgctgcg
    16321 accgctgctt acggctggtg aacgggcagt tgcaggagga agcatgattg cacgttggtt
    16381 ctggcgcgaa tggcgttcgc cgtcgctatt aattgtctgg ctggcgctaa gcctggcggt
    16441 ggcctgcgtg ctggcgctgg gcaatatcag cgatcgcatg gagaagggct taagccagca
    16501 aagccgtgag tttatggcgg gcgatcgggc gttgcgcagt tcacgcgaag tgccgcaagc
    16561 gtggctggag gaagcgcaaa agcgcggcct gaaagtcggc aagcagctga ctttcgccac
    16621 aatgaccttt gcaggcgaca caccgcagct ggcgaacgtc aaagcggtgg atgatatcta
    16681 cccgatgtat ggcgatctgc aaactaatcc ccctggcctg aaaccgcagg cgggcagcgt
    16741 attgctggcc ccacgcctga tggcactgct taacctgaaa acgggcgaca ccattgacgt
    16801 gggcgatgcc accttgcgga ttgccggaga agtgattcag gaaccggatt ccggttttaa
    16861 ccccttccag atggctccgc gtctgatgat gaatctggcg gatgtcgata aaaccggagc
    16921 cgtgcagccg gggagtcggg tcacctggcg ttataaattc ggcggcaacg agaaccagct
    16981 cgacggctat gagaaatggt tgttacctca gcttaaaccc gaacaacgct ggtacggtct
    17041 ggaacaggac gaaggcgcgc tggggcgatc gatggaacgc tcgcaacagt tcctgctgct
    17101 ttcggcgctt ctgaccttgc tgctggcagt ggcagcggtg gcggtagcga tgaatcatta
    17161 ctgtcgcagt cgctacgatc tggtggcgat cctcaaaacg ctgggggcag ggcgagcgca
    17221 actgcgtaag ctaatcgtcg gtcagtggtt gatggtgctg acgctttcag ccgttaccgg
    17281 tggggccata ggcctgttgt tcgaaaacgt gttgatggtg ctgctcaagc ccgttctgcc
    17341 tgctgcacta ccgccagcca gcctctggcc gtggctgtgg gcgcttggca ccatgacggt
    17401 catctcgctg ctggtggggc tacgaccata ccgcttgttg ctggcaacgc agcctttacg
    17461 cgtattacgt aatgatgtgg tagcgaacgt ctggccgctg aagttttatc tgccgattgt
    17521 cagtatggtg gttgtgctgc tgctcgccgg attaatgggt ggcagcatgc tgctttgggc
    17581 ggtgctggcg ggcgcggtag tactggcttt gctgtgcggt gtgctgggct ggatgcagct
    17641 gaatgtactt tgccgcatga cgctgaaatc gctacctctg cgcctggcgg ttagccgcct
    17701 gttacgtcag ccgtggtcaa cgttaagcca gctttcggca ttttcgctct cctttatgct
    17761 gctggcgctg ttgctggtat tgcgttgcga tctgctcgac cgctggcagc agctaccgcc
    17821 agaaagcccg aactactttt taattaacat cgccacagaa caggtagcac cgctaaaagc
    17881 gttcctcgcg gaacatcata tagtcccgga atcattttat ccggtagtgc gggcgcggct
    17941 gacggcgatt aacgataagc cgacagaagg taatgaagat gaggcgctta atcgcgaact
    18001 caatcttacc tggcaaaata cgcggcccga tcataatccg attgtcgccg gtaactggcc
    18061 gccaaaagcc gatgaagtgt cgatggaaga ggggctggcg aaacgcttaa acgttgccct
    18121 cggtggtacc gtgactttta tgggcgatac ccaggagttc cgcgctaaag tgaccagcct
    18181 gcgcaaagtg gactgggaaa gtctgcggcc taatttctat tttattttcc cggaaggggc
    18241 attagacggg caaccgcaga gctggcttac cagtttccgc tgggagaatg gcaacggcat
    18301 gttgacgcaa ctcaaccgcc agttcccgac cattagcctg ttagatattg gcgcgatttt
    18361 aaaacaggtc ggttaggtgc tggagcaggt aagtcgggcg ctggaggtga tggtggtgct
    18421 ggtcaccgcc tgcggtatgt tgctgttgct ggcacaggtg caggtgggaa tgcgccagcg
    18481 tcatcaggag ctggtggtat ggcgcacact cggtgcgggg aaaaaactgc tgcgcacgac
    18541 attgtggtgt gagttcgcca tgcttgggtt tgtttccggc ctggtggccg caattggtgc
    18601 ggaaacggca ctggcagtgt tgcaggcgaa agtgtttgat ttcccgtggg agccagactg
    18661 gcgattgtgg attgttctgc cgtgcagcgg tgcgctgctg ctgtcgcttt tcggcggctg
    18721 gctgggtgcg cgactggtta agggtaaggc gctgttcagg cagtttgcgg ggtgatgaga
    18781 gtaaataagg ggcgttctgg ttgaatggaa cgccttgttt aaattaattc tacaatatgt
    18841 ttcattgatg atgttattga attggtgatt tcatatcttt ctattgctga tattaatctg
    18901 aattggtgga gtttaataat tttaaatata gccctacaaa aataactcag actaataaaa
    18961 tcataaatca tatgcgttga atggatatta tccatatagt gaatttgttg atgatgaatt
    19021 catctgtgct aaaaatttta gtttaataaa atattgaaag tgacctgtaa taacagttat
    19081 tgttgattga gaacaaataa gtttatgtga aaaatatata aatacattag ctggtcatgt
    19141 gtgtcatttt atttttttgt tgctaacaca gggatatgaa caataactaa aagggcactt
    19201 tatatgagcg gaaaaccagc ggcgcgtcag ggagatatga ctcagtatgg cggtcccatt
    19261 gtccagggtt cggcaggtgt aagaattggc gcgccaactg gcgtggcgtg ctcggtgtgt
    19321 ccgggcggga tgacctcggg caacccggtg aacccgctgc tcggtgcaaa agtacagccc
    19381 ggcgagacgg accttgcgct gcccgacccg ctgccgttca ttctctcccg cacctacagc
    19441 agctaccgga cgaagacgcc tgcgccagtg ggaacttttt ggccccggct ggaaagcgcc
    19501 ttctgatatc cgcttacaga tacgcgatga tgcactggta ctcaacgaca acggcgggcg
    19561 gagcattcac tttgagccgc tgctgccggg gaaggctgtg tacagccgca gtgagtcaat
    19621 gtggctggtg cgtggcggta aggcagcgca gccggacggc cacacgctgg cacgactgtg
    19681 ggcgtcgctg ccgccggata tccggttaag cccgcatctt tacctggcga ccaacagcgc
    19741 acaggggccg tggtggatac tggggtgatc tgagcgggtg ccgggtgctg aggacgtact
    19801 gccagcgccg ctgccgccgt accgggtgct taccgggatg gcggaccgct tcgggcggac
    19861 gctgacgtac cggcgtgagg ccgcaggcga cctggccggg gaaatcaccg gcgtgacgga
    19921 cggtgccggg cgggagttcc gtctggtgct gaccacacag gcgcagcagg cggaagaggc
    19981 ccggcagcag gcggtcgtcg ccggagcaaa acgaccggac attcctgatt cactgccgga
    20041 gagcacagag tacggccccg acaggggtat ccgcctttcg gcggtgtggc tgatgcacga
    20101 cccggcatac ccggagagcc tgcccggtgc gccactggcg cggtacacgt atacggaagc
    20161 cggtgaactg ctggcggtat atgaccgcag caatacgcag gtgcgcgctt tcacgtatga
    20221 cgcgcagcac ccgggccgga tggtggcgca ccgttacgcg ggaaggccgg agatgcgcta
    20281 ccgctacgac gatacggggc gggtggtgga gcaactgaac ccggcagggt taagctaccg
    20341 ctatctttat gagcaggacc gcatcaccgt caccgacagc ctgaaccggc gtgaggtgct
    20401 gcatacagaa ggcggggccg ggctgaaacg ggtggtgaaa aaagaactgg cggacggcag
    20461 cgtcacgcgc agcgggtatg acgcggcagg aaggctcacg gcgcagacgg acgcggcggg
    20521 acggcggaca gagtacggtc tgaatgtggt gtccggcgat atcacggaca tcaccacacc
    20581 ggacgggcgg gagacgaaat tttactataa cgacgggaac cagctgacgg cggtggtgta
    20641 cccggacggg ctggagagca gccgggaata tgatgaaccg ggcaggctgg tatcggagac
    20701 atcgcgcagc ggggagacag tacgctaccg ctacgatgac gcgcacagtg agttaccggc
    20761 gacgacaacg gatgcgacgg gcagcacccg gcagatgacg gcggttcacc gcgaggaagg
    20821 tatcagcctt taccgccact atgacaaccg tggccggtta acctcggtga aagacgcaca
    20881 gggccgtgaa acgcagtatg agtacaacgc cgcaggcgac ctgactgccg ttatcacccc
    20941 ggacggcaac cggagcgaga cacagtacga tgcgtgggga aaggcggtca gcaccacgca
    21001 gggcgggctg acgcgcagta tggagtacga tgctgccgga cgtgtcatca gtctgactaa
    21061 cgagaacggc agtcacagcg acttcagcta cgatgcgctg gaccggctgg tacagcaggg
    21121 cggctttgac gggcggacgc aacgttatca ttatgacctg accggaaaac tcacgcagag
    21181 tgaggatgag gggcttgtca ccctctggca ctacgatgcg tcggaccgca tcacgcaccg
    21241 gacggtgaac ggcgacccgg cagagcagtg gcagtatgac ggccacggct ggctgagaga
    21301 aatcagccac ctgagcgaag gccaccgtgt cgctgtccac tacggctacg acgataaggg
    21361 ccgcctgacg ggcgaacggc agacggtgga gaacccggag acgggggaac tgctgtggca
    21421 gcatgagacg aaacacgcct acaacgaaca ggggctggca aaccgcgtca cgccggacag
    21481 cctgccgccg gtggagtggc tgacctacgg cagcggttac ctggcgggca tgaagctggg
    21541 cgggacgccg ctggtcgagt atacgcggga caggctgcac cgtgagacgg tgcgcagctt
    21601 cggcagcatg gcaggcagta atgccgcata tgaactgacc agcacataca cccccgcagg
    21661 ccagttacag agccagcacc tgaacagcct ggtgtatgac cgtgactacg ggtggaatga
    21721 caacggcgac ctggtgcgca tcagcggtcc gcgacagacg cgggaatacg gctacagcgc
    21781 cacgggcagg ctggagagcg tgcgcaccct cgcaccggac ctggacatcc gcatcccgta
    21841 tgcgaccgac ccggcgggaa accggctgcc ggacccggag ctacacccgg acagcacgct
    21901 cacggcgtgg ccggataacc gcatcgcaga ggatgcgcac tatgtttacc actatgatga
    21961 atacggcaga ctgacggaga agacggaccg catcccggcg ggtgtgatac ggacggacga
    22021 cgagcggacc caccactacc actacgacag ccagcaccgc ctggtgttcc acacgcggat
    22081 acagcatggc gagccactgg tcgagagccg ctacctctac gacccgctga gccgccggac
    22141 ggggaaacgg gtgtggcgtc gcgagcgtga cctgacgggg tggatgtcgc tgtcgcgtaa
    22201 accggaggtg acgtggtacg gatgggacgg cgaccggctg acgacggtac agaccgacac
    22261 cacgcgtatc cagacggtat accagccggg gagcttcgcg ccgctcatcc gcatcgaaac
    22321 agacaacggt gaacgggaga aagcgcagtg ccgcagcctg gcggagaagt tacagcagga
    22381 agggagcgaa gacggtcacg gtgtggtgtt cccggctgaa ctggtggggc tgctggacag
    22441 gctggagggg gaaatccggg caaactgcgt gagcagtgaa agccgccagt ggcttgcgca
    22501 gtgcgggctg acggtggagc ggctggcggc acagatagag ccggtttatt tgcctgagcg
    22561 aaaaatacat ctttatcact gtgaccaccg gggcctgccg ctggcgctta tcagtgaaga
    22621 cggcaatacg gcgtggagcg cggaatatga tgaatggggc aaccagctta atgaggagaa
    22681 cccgcatcac ctgcaccagc cgtaccggct gccggggcag cagtatgata aggagtcggg
    22741 gctgtactac aaccggaacc ggtactacga tccgttgcag gggcggtata tcactcagga
    22801 cccgataggg ctggaggggg gatggagtct gtatgcgtat ccgctgaatc cggtgaatgg
    22861 tattgatcca ttagggttaa gtcccgcaga tgtagcgcta ataagaagaa aagatcaact
    22921 aaaccatcaa a
//
LOCUS       DADLFA010000051        22917 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000051 DADLFA010000000
VERSION     DADLFA010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22917
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.051"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..418)
                     /gene="yeeE"
                     /locus_tag="JQH65_001676"
     CDS             complement(<1..418)
                     /gene="yeeE"
                     /locus_tag="JQH65_001676"
                     /inference="COORDINATES: protein motif:HMM:NF016064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HAY6139128.1"
                     /translation="MTYEAGAFPWPGTVIGGYIFGLGIVLAGGCATGTWYRAGEGLIG
                     SWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVAVLLVITLW
                     VVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVT"
     gene            complement(428..610)
                     /locus_tag="JQH65_001677"
     CDS             complement(428..610)
                     /locus_tag="JQH65_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001467426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HAY6139129.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALI"
     gene            complement(789..2147)
                     /gene="plaP"
                     /locus_tag="JQH65_001678"
     CDS             complement(789..2147)
                     /gene="plaP"
                     /locus_tag="JQH65_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HAY6139130.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(2137..2199)
                     /gene="yoeI"
                     /locus_tag="JQH65_001679"
     CDS             complement(2137..2199)
                     /gene="yoeI"
                     /locus_tag="JQH65_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HAY6139131.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(2414..3343)
                     /locus_tag="JQH65_001680"
     CDS             complement(2414..3343)
                     /locus_tag="JQH65_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY6139132.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQHFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(3389..4213)
                     /locus_tag="JQH65_001681"
     CDS             complement(3389..4213)
                     /locus_tag="JQH65_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY6139133.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGSGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGMVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(4296..4550)
                     /gene="yoeB"
                     /locus_tag="JQH65_001682"
                     /pseudo
     CDS             complement(4296..4550)
                     /gene="yoeB"
                     /locus_tag="JQH65_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
     gene            complement(4547..4798)
                     /gene="yefM"
                     /locus_tag="JQH65_001683"
     CDS             complement(4547..4798)
                     /gene="yefM"
                     /locus_tag="JQH65_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HAY6139134.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            5081..5131
                     /gene="hisL"
                     /locus_tag="JQH65_001684"
     CDS             5081..5131
                     /gene="hisL"
                     /locus_tag="JQH65_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HAY6139135.1"
                     /translation="MTRVQFKHHHHHHHPD"
     gene            5277..6176
                     /gene="hisG"
                     /locus_tag="JQH65_001685"
     CDS             5277..6176
                     /gene="hisG"
                     /locus_tag="JQH65_001685"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HAY6139136.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            6182..7486
                     /gene="hisD"
                     /locus_tag="JQH65_001686"
     CDS             6182..7486
                     /gene="hisD"
                     /locus_tag="JQH65_001686"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HAY6139137.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDEALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPAAD
                     MARRVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSLESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            7483..8553
                     /gene="hisC"
                     /locus_tag="JQH65_001687"
     CDS             7483..8553
                     /gene="hisC"
                     /locus_tag="JQH65_001687"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HAY6139138.1"
                     /translation="MSIVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYGVSAETIGVECRTVPTLDNWQLDLQGISDKLEGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     ECLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            8553..9620
                     /gene="hisB"
                     /locus_tag="JQH65_001688"
     CDS             8553..9620
                     /gene="hisB"
                     /locus_tag="JQH65_001688"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HAY6139139.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAIDRANSYVIGDRATDIQLAENMGITGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLAHCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            9620..10210
                     /gene="hisH"
                     /locus_tag="JQH65_001689"
     CDS             9620..10210
                     /gene="hisH"
                     /locus_tag="JQH65_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HAY6139140.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            10210..10947
                     /gene="hisA"
                     /locus_tag="JQH65_001690"
     CDS             10210..10947
                     /gene="hisA"
                     /locus_tag="JQH65_001690"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HAY6139141.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYATQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            10929..11705
                     /gene="hisF"
                     /locus_tag="JQH65_001691"
     CDS             10929..11705
                     /gene="hisF"
                     /locus_tag="JQH65_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015853929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HAY6139142.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEVVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            11699..12310
                     /locus_tag="JQH65_001692"
     CDS             11699..12310
                     /locus_tag="JQH65_001692"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HAY6139143.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVAFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLLLVNPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(12407..13384)
                     /gene="wzzB"
                     /locus_tag="JQH65_001693"
     CDS             complement(12407..13384)
                     /gene="wzzB"
                     /locus_tag="JQH65_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HAY6139144.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWASTAIVTQPDVGQIAGYTNAMNVIYGSAIPKVSDIQASLIGRYSTA
                     FSALAETLDNQEEAEKLTIEPTVKNQSLPLAVSYVGETPEGAQKQLAKYIQQVDDQVN
                     EELGKDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIDNLDVDKLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(13531..13623)
                     /locus_tag="JQH65_001694"
                     /pseudo
     CDS             complement(13531..13623)
                     /locus_tag="JQH65_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            13793..14797
                     /locus_tag="JQH65_001695"
     CDS             13793..14797
                     /locus_tag="JQH65_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015572392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HAY6139145.1"
                     /translation="MKFLVTGAAGFIGFYLSKRLLAAGHQVVGIDNLNDYYDVNLKQA
                     RLDLLKHDNFSFYKIDLADREKMAALFADERFERVINLAAQAGVRYSLENPNAYADAN
                     LIGFLNILEGCRHNNVQHLLYASSSSVYGMNRKMPFSTDDSVDHPVSLYAATKKANEL
                     MAHTYSHLYGLPTTGLRFFTVYGPWGRPDMALFKFTKAMLEGKSIDVYNFGKMKRDFT
                     YIDDIAEAIIRLQDVIPEKNPQWAVETGSPATSSAPYRVYNIGNSSPVELMDYINALE
                     EALGIEANKNMMPLQPGDVLETSADTKALYDVIGFKPETSVKEGVKNFVEWYRNFYKV
                     "
     gene            complement(14863..16029)
                     /gene="ugd"
                     /locus_tag="JQH65_001696"
     CDS             complement(14863..16029)
                     /gene="ugd"
                     /locus_tag="JQH65_001696"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HAY6139146.1"
                     /translation="MKITISGTGYVGLSNGILIAQNHEVVALDIVQAKVDMLNKRQSP
                     LVDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFNTSSVEAVIR
                     DVTEINPNAVMIIKSTIPVGFTESIKERFGIENVIFSPEFLREGKALYDNLHPSRIVI
                     GEQSERAKRFAALLQEGAIKQDIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADSILARKPKVVGVYRLIMKNGSDNFRASSIQGIMKRIKAKGVPVIV
                     YEPAMKEDDFFRSRVVRDLDAFKQEADVIISNRMSADLADVADKVYTRDLFGND"
     gene            complement(16278..17684)
                     /gene="gndA"
                     /locus_tag="JQH65_001697"
     CDS             complement(16278..17684)
                     /gene="gndA"
                     /locus_tag="JQH65_001697"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAY6139147.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(17812..18867)
                     /locus_tag="JQH65_001698"
     CDS             complement(17812..18867)
                     /locus_tag="JQH65_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020580627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAY6139148.1"
                     /translation="MSNTAIIGTVGVPASYGGFETLVENLIETEKVNYIVYCSSKNYP
                     KKLSTYKNAKLVYIPLSANGISSVIYDAFSILHAIVFRKVNTLLILGVSGCFILPIIK
                     KISSVKIITNIDGVEWRRDKWGRFAKKFLKWSEKIAVKNSNIVISDNKEIANYVCSEY
                     MVNSQVIAYGGDHSLVFETKRCNDVENSESKYALALCRIEPENNVHVILESFSKVTKK
                     LKFIGNWGSSDYGRSLKKKYSKYNNIEIIDPIYDVEQLNIIRSGCEFYVHGHSAGGTN
                     PSLVEMMFFAKPIICFDCKYNRYTTFEQACYFRDLKDLIIKIESPKERTQGVKLKSLA
                     EKHYTWNRIREQYEQLL"
     gene            complement(18860..20152)
                     /gene="wzy"
                     /locus_tag="JQH65_001699"
     CDS             complement(18860..20152)
                     /gene="wzy"
                     /locus_tag="JQH65_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060634342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O53 family O-antigen polymerase"
                     /protein_id="HAY6139149.1"
                     /translation="MLAWAPKLLLLFFLLSGLIKAISLYININLNYVFIPLLILASLF
                     YLLINRTIKMKAQGACFIFFFSISAILFLVNNIVVNNVYTQLQLMLAVTIQYIFPIII
                     ICLAGSGKLKLDIFDSLINIITKLTFLSLLIFGLCVMYDSKMVEEFYSQLYNHGLIIN
                     PFQTSEEGITLRFSGIFSSGFILASYCCFAIIYYYFNKSVNRIKFAFLFVGFVIMVVL
                     TYNRNGMLAFAVTSLFIMIHTYLRRYYLTLANLYYVFLIGILFIAPSFILYFGGDIQN
                     SYSSIDYSAFTKLSTLFSRIDAWLQVLNINDVSRLLFGSGLVQGLGEDDMNFYVDNGY
                     LYLLHQSGLIALVLYIATWSYMFISLSKNLKKRRNHNFVSVDEINICLLLILVSFTLA
                     LLNNLFFEPVFLILVLMKCVTVNNKSSSIYEKNEQTYE"
     gene            complement(20156..21250)
                     /locus_tag="JQH65_001700"
     CDS             complement(20156..21250)
                     /locus_tag="JQH65_001700"
                     /inference="COORDINATES: protein motif:HMM:NF025072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAY6139150.1"
                     /translation="MKKIAILLPGPRREPVGGYKVLYQYGNYLASVGWQIDFIYLTNR
                     IVCYNRFSGLVGKIKAKLFSLLKFYKWFDFTSSNINHYVIKSLKKRDIENFNIVICSS
                     VETFIYANSSCRVNHKKLVYFVQDYENWNIPENILNYTLSHKDVDYITISDDLVKKIT
                     KMGGDVSLTLYNGINKNDFFVNIPWNERDRASFLFLYHPSERKGCDLLLRAIQKIHEV
                     DSSIKFSCFSAYTKPENFPSFIHYVYQPTVKQLNNLYNENRYFICSSIYEGFGLTAAE
                     ASFSGAIVVTTRNGGVEQFIKNEVTGFVLKERTLNSFMVAIMKLSQKDEELNLFAIES
                     SKKVRIQLDLDINFKKIDSYFKARYNNKRN"
     gene            complement(21251..22480)
                     /gene="wzx"
                     /locus_tag="JQH65_001701"
     CDS             complement(21251..22480)
                     /gene="wzx"
                     /locus_tag="JQH65_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060634343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O53 family O-antigen flippase"
                     /protein_id="HAY6139151.1"
                     /translation="MVKKIVNQFIYQFGNYILPFLIIPFLINKLSIAVYKDFVVIQSL
                     VLFFAVLINYGLDLFGVRYISNNVKHLRKCKSFLYISILSRLCIFIFCSFFLLVSLMA
                     LTKPDLLYWLLCFFWMLSFVFQSNWYLQGCGDFKLITFYGLLPKLIVYPFVFILVNND
                     NDAWVYLFLCAIANYASNILMMIHIRRKLNVVAFKRTNLLRNVWIFLVKGWYIFLSQA
                     SVTLISYANIFFLPLVLEPKSFVVFSTAERIVKVLSITTTPILNVIFPHISSLIENNK
                     QRVFIMISRISLFSLIVFLLCYTLYVFIGYYLIQLIFPTISQELYSVLLLLLFNVFFV
                     FQNNLFGTQIGLNYGKDKSFTKIIAINGILNIIAMPVFSKLYHLHGTILTSLCIQLSI
                     LICMYMLAKKCGYKFKV"
     gene            complement(22461..>22917)
                     /locus_tag="JQH65_001702"
     CDS             complement(22461..>22917)
                     /locus_tag="JQH65_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAY6139152.1"
                     /translation="FLNKLSKKFSIIIWGKFFDDSIRDKLNENIKIAGAFKYSDLPSI
                     MKSFKVGLLPYLPEKSHDESPLKMYEYFKYGIPCLSSMNFEIKSRWFVNYLEYSKRDL
                     LYEKINELIVSNNSESIKASIQKEWLFEIKVNKIIEDIIRRKECGKKNS"
BASE COUNT         6546 a         5369 c         5022 g         5980 t
ORIGIN      
        1 cagtgacaaa gggatgccag cgtttttcaa acagaatatg agcgatcccg gttcggcgcg
       61 gcggtaaggt cgcgactttg agttttggct tcttcaactc tttcatcacc acccagagcg
      121 ttatcaccag caaaacggca accaacggcc acacagacaa attaaaagtc tcagcaatag
      181 agttatgttc agtactgtag tgctgcaagg tttgatttaa accactggca tgtggagaac
      241 gcatcaccgc actcatcacc atataagtga aaagcgcgat ccaactgccg atcaatccct
      301 cacccgcgcg ataccaggtc ccggtggcac atccacccgc cagaacaatt cccagcccga
      361 agatataccc acctataaca gtaccaggcc acgggaacgc cccggcttcg taagtcagta
      421 ggcccgccta aattaacgca aaaaccccca cactttgtac cgaaattgca atcagcaagg
      481 cgtaaaacat gcgattattt ttcgcgatat acatatcgcg aaaaccacca gtcaggcaaa
      541 aacgcccacg ctgcatgaca aatcccagca gagcaccaca aattagcccg cttaatatca
      601 ttgaaaacat aaattaatta accagatgaa tgttaatgag gaaattattc atgactggtg
      661 gaatgcagac caataaccaa attctctaaa ttagaacaaa tggttcttaa tgaggatttc
      721 gataaaaaag aataaaaaaa ccggagccaa atattcattc gactccggtt gattattaga
      781 aaagatgatt atgctacgtc ttcgtactgc ggaaccgggt tacggaagct cttggtgacg
      841 caagccaggt aaatcagacc gatagcagcc cagatcagac ccagaaccat tgagctttct
      901 tccaggttaa cccacagcgc accaacggtc agcgcaccac acatcggcag gaacagatac
      961 tggaagtgat ctttcagcgt cttattacgc ttctcacgga tccagaactg cgagatgacc
     1021 gacaggttaa cgaaggtgaa cgccaccagc gcaccaaagt taatcagcgc cgttgccatt
     1081 accaggtcga agttgattgc cagcagggca atcgcgccaa ccaggatgat gttcatcgcc
     1141 ggagtacgcc atttcgggtg tacataaccg aagaagcttt tcgggaatac gccgtcacga
     1201 cccattacgt acatcagacg cgctacgcct gcatgcgccg ccataccgga cgccagtacg
     1261 gtaatggtgg agaagatcag cgcgccaacc tggaacgctt tacccgcaac gtacagcatg
     1321 atttcaggct gtgacgcatc cggatcttta aagcgagaga tatccgggaa gtacagctgc
     1381 aggaagtaag ttgcaaagat gaagatcatg ccgccgatca gcgcggtcag gaaaatcgca
     1441 cgcgggatca cgcgctctgc atctttggtt tcttccgaca ggttgctgat gccgtcaaag
     1501 ccggtaaagg agaagcacag gattgtcgcc ccggtaatca tcgggataac atgcgcatcg
     1561 ccagaccaga atggacgagt gctcgccagc gtaccagcgc cttcaccttc aaatacgcca
     1621 taaacaacca tgcccagaat caccgcgatc agcaccacct gcaacacgac gattacagtg
     1681 ttgaagttcg ctacggattt cagactacgc aggttaaagg cggtcatgaa ggccaccaac
     1741 gccaccacaa acatccacga tgggatggaa ggcaccagag cttcaaaata gattttcgcc
     1801 agcagaatgt tgatcatcgg cgcgaacaga tagtcgagca gagaagacca acccaccata
     1861 aagccgacag tcgggctaat ggatttctgg gcgtaagtgt atgcagagcc agcagaagga
     1921 tagcggcgaa ccagcttccc gtagctcaga gccgtaaaca ggatcgcaat caatgcgaac
     1981 gcataggctg tcggcacatg accatccgtg aggcctgaaa cgataccaaa agtatcaaac
     2041 agcgtcatcg gctgcatata ggcaagaccc atcattacaa ccggaactaa cgtaagcgtt
     2101 ttacgcaatt ccacgcgaga ggtgtttgga gtaacgttat gcgacatggt cattctcctt
     2161 tacggtgata accgtcgcgt aagcaaaaaa ttgccccatt tttttggatt cctcagcgac
     2221 aacaactgtc gatttttagt aaatatctat ccggtacgaa gcccggcctc ttggtatgaa
     2281 tgattggttt gaagcaaaaa ataaccgacg ctgatgaaac gtcggttttt agtcattttt
     2341 tgacagcggc gcattgtgcc taaatggggg ggaaatgaca agagaatgag aggcttgtca
     2401 taataatttt tctttaaatg gctgattttc cgtcatcaga ttttgcataa acaccggcga
     2461 tcgcactatt tgctaaaatt tcatcccgcc accagccact ggcaagtcct gcggtctgtt
     2521 cattccagcc aatccagacg ggttcaaaga gagtttgggc gaccactttc ttctcgatta
     2581 acgcgccact atcgagaaaa tgttgcgcca gataacgggg taaatagccg cagccgaggc
     2641 cgctaatttg cagctccagc ttggttttaa aatcgaaaac ggtaatggct tcttgttcgt
     2701 cgagaagttg tgaggccgta gaagcgcctg cctgcgcagt atcccccacc acaatcgccc
     2761 gatatcgctt aataatacga cgatttaacg gctcttcttc cagggctaac ggatgatgag
     2821 gcgcgaccgc aaagacttgt tccagatcgc ccaaccgtga aaaaccaaac tcactggaag
     2881 atggtggctc atgcatcgcg ccaacaataa tatccgctcg cccctgagtc agggcatccc
     2941 atgaaccacc cagtacgcca ttgataaact tcaggcgcgt cacgctatga tgttgataaa
     3001 aagcttcaat cagtggcgct agcagagaaa aaggaaacgt gtcgtccacg ccaatcacca
     3061 gctcgttttc ccagccttca tgcagtttga tcgcctgctt ttccagttcc cgcacggtat
     3121 gcagaacttc ccgccctttc tctaataaca tttttccggt gcgggtgaat ttagcgcggt
     3181 gaccgctacg atcaagcaat tgaatattaa ggtcgctttc cagcttgtga acggtgtaac
     3241 tcagagcgga cggcgtcttg tagagtttgg ccgacgccgc cgcaaaactg ccttcttttt
     3301 ctaaggcatc gaggatcatc aacacatcca gcagtggttt catacacgcc cccttgcagt
     3361 gtatgcggcg tcctgctggc gtgatcgttt actccagcgg cattaccagc ggatcgggat
     3421 actggtattc aaatcccagt tcattacaaa tccgactgcc atcaataatc ttacctttgc
     3481 cgctgtccag actatttctg aactgcggag gttccagccc cagtaaacgg gccatctgcg
     3541 gatagaaaac attacgcgca gggtgagctg gcgcacatat attatagatg tgtccgcctt
     3601 taggtgcctg taacaacaga gtgatagcgc caataacatc ttctaaatgg actaaattaa
     3661 caccatgttc accatcaggc gcggtttttc cggcaaagaa gcgtccggga tgacgccccg
     3721 gtccgaccag gcccgcaaga cgcagaatat cgaccgaagt accgggtaaa ttgtgcagcc
     3781 agtcttcgag ttcttctaat actcgtccac tgttggttac cggattacgc ggggtagttt
     3841 ctttcaccat gccttgcgca tcgccataga cagatgttga gctggtaaaa ataattcggg
     3901 gaatacgatg ggccagcgcg ctatccacta actcttgtac cgcttgtaaa tagaactcat
     3961 cgccggagcc gctacgacgt gccggaagcg taatgaccag tgcatcggca tccatcaggg
     4021 catccagatc gtcagaatcg caaactaact caggctccat gcgaagcaga tagctatcaa
     4081 tgccactcat tcgggccgct tcgacaccat cttgtgtggt tttactcccg gtgacttgcc
     4141 agcctcgcgc tgaaagtgac atcgccagcg gcatgcctaa ccaccctaac ccgacaattg
     4201 cgaccttttt catccgttat ctcctgatac accttagatc tataaggcta cgctagcgta
     4261 tcaaaactga caattcattc tatgaatgaa tctgttcaat aatgataacg acatgctgca
     4321 atgagcagtg aatcgtcggt aaccgcgtat accagacggt gctcctctgt aatgcgtcgg
     4381 gactagaaac ctgacaaatt atgtttcagg ggttctggct tccccttacc ttcaaatggc
     4441 gttctgcggg tatctttgat aagttcattg atctttttaa caattcgctt atctgtttcc
     4501 tgccagtaca gataatcatc ccatgattcc tcagaccaga ttagtttcac tcaataatgt
     4561 ccttttctgt tcctttgcct gatttcaggc tatcgattga gtccatcaat ctccgggcgt
     4621 tagcggggga acgcagtaga taagccgtct cttccagcga gttgtattct tcgagtgaca
     4681 tcagaacaca agcctctcca ttctgacgag taataaggat cggggcatga tcttcaacgg
     4741 ctttcatcat tgttgccgac aaattctgac gcgcttcgct gtagctaatt gtacgcatgt
     4801 caatctcctc ttttgtacag ttcattgtac aatgatgggc gttaattaac tatttattaa
     4861 ttagtttgta gatcaaggta ttgtcagtga gacgaaaatc caggcttcgc tatttttggt
     4921 gccgtcagct tagaggacag tcctcttagc cccctccttt ccccgctcat tcattaaaca
     4981 aatccatcgc catagaatat ataaaaaagc ccttgctttc taacgtgaaa gtggtttagg
     5041 ttaaaagaca tcagttgaat aaacattcac agagactttt atgacacgcg ttcaatttaa
     5101 acaccaccat catcaccatc atcctgacta gtctttcagg cgatgtgtgc tggaagacat
     5161 tcagatcttc cagtggtgca tgaacgcatg agaaagcccc cggaagatca ccttccgggg
     5221 gcttttttat tgcgcggttg ataacggttc agacaggttt aaagaggaat aagaaaatga
     5281 cagacaacac tcgtttacgc atagctatgc agaaatccgg ccgtttaagt gatgactcac
     5341 gcgaattgct ggcgcgctgt ggcattaaaa tcaatcttca cacccagcgc ctgatcgcga
     5401 tggcagaaaa catgccgatt gatattctgc gcgtgcgtga cgacgacatt cccggtctgg
     5461 taatggacgg cgtggttgac ctcgggatta tcggcgaaaa cgtgctggaa gaagagctgc
     5521 ttaaccgccg cgcccagggt gaagatccac gctactttac cctgcgtcgt ctggatttcg
     5581 gcggctgccg actttcactg gcaacgccgg ttgatgaagc ctgggatggc ccgctctcct
     5641 taaacggtaa acgtatcgcc acctcttatc ctcacctgct caagcgttat ctcgaccaga
     5701 aaggtatctc ttttaaatcc tgcttactga acggttctgt tgaagtcgcc ccgcgtgccg
     5761 gactggcgga tgcgatttgc gatctggttt ccaccggtgc cacgctggaa gctaacggcc
     5821 tgcgcgaagt cgaagttatc tatcgctcga aagcctgcct gatacaacgc gatggcgaaa
     5881 tggaagaatc caaacagcaa ctgatcgaca aactgctgac ccgtattcag ggtgttatcc
     5941 aggcgcgtga atcaaaatac atcatgatgc acgcaccgac cgaacgtctg gatgaagtca
     6001 tcgccctgct gccaggtgcc gaacgcccaa ctattctacc gctggcgggt gatcaacagc
     6061 gcgtagcgat gcacatggtc agtagcgaaa ccctgttctg ggaaacgatg gaaaaactga
     6121 aagcgctggg tgccagttca atcctggtcc tgccgattga gaagatgatg gagtgatcgc
     6181 catgagcttt aacacaatca ttgactggaa tagctgtact gcggagcaac aacgccagct
     6241 gttaatgcgc ccggcgatct ccgcttctga aagcattacc cgcactgtta acgatattct
     6301 cgataacgtg aaagcacgcg gcgatgaggc cctgcgggaa tacagcgcga agtttgataa
     6361 aaccacggtt accgcactga aggtgtctgc tgaggaaatt gccgccgcta gcgaacgcct
     6421 gagcgacgag ctaaaacagg cgatggcggt ggcagtgaag aatattgaaa ccttccacac
     6481 tgcgcaaaaa ctgccgccgg tagatgtaga aacgcagcca ggcgtgcgtt gccagcaggt
     6541 cacgcgcccg gtcgcttcag ttgggttgta tattcctggc ggctccgccc cgctcttctc
     6601 aacggtatta atgctggcga ctccggcgcg tattgcgggc tgtaaaaaag tggtgctgtg
     6661 ctcaccgccg ccgattgccg atgagatcct ttatgcggcg cagctgtgcg gtgtgcagga
     6721 cgtgtttaac gtcggcggcg cacaggccat tgccgcactg gcgtttggta cggaatctgt
     6781 gccgaaagtg gacaaaatct tcgggccggg caacgccttt gtcaccgaag cgaaacgtca
     6841 ggtgagccag cgtctggacg gtgcggcgat cgatatgccc gcaggcccgt cggaagtgtt
     6901 ggtcattgcc gacagtggcg caacgccgga tttcgtggct tctgatttgc tttctcaggc
     6961 tgaacacggc ccggactcac aggtgatttt actgacgccc gctgctgata tggcgcgtcg
     7021 cgttgccgag gctgtcgaac gccagctggc agaactgccg cgtgctgaaa ccgcccgtca
     7081 ggcactgaac gccagccgcc tgatcgtgac taaagattta gcgcagtgcg tagagatctc
     7141 caaccagtac ggcccggagc acctgatcat ccagacccgc aacgcccgcg atctggtcga
     7201 tggcattacc agtgccggtt cggtatttct tggtgactgg tcactggaat cggcaggtga
     7261 ttacgcctcc ggcaccaacc acgttctgcc gacttacggt tacaccgcca cctgttccag
     7321 cctcgggctg gcagatttcc agaagcgcat gaccgtgcag gaactgtcga aagaaggttt
     7381 ctccgcgctg gcttcgacca ttgaaacatt ggccgccgcc gagcgcctga ccgcccataa
     7441 aaatgccgtt actttacgtg ttaacgccct taaggagcaa gcatgagcat cgtgactatt
     7501 accgatttag cgcgtgaaaa cgtgcgcaac ctgacgccgt atcagtcggc gcgtcgtctg
     7561 ggcggtaacg gcgatgtctg gctgaacgcc aacgaatatc ccacagccgt ggaatttcag
     7621 cttactcagc aaacgctcaa ccgctacccg gaatgtcagc cgaaagcggt gattgaaaat
     7681 tacgcgcaat atgcaggcgt aaaaccggaa caggtgctgg tcagccgtgg cgcggacgaa
     7741 ggtattgaac tactgattcg cgctttttgc gaaccgggta aagacgccat cctctactgc
     7801 ccgccaacgt acggcatgta cggcgtcagc gccgaaacca ttggcgtcga gtgccgcaca
     7861 gtgccgacgc tggacaactg gcaactggat ttacagggca tttccgacaa gctggaaggc
     7921 gtaaaagtgg tctatgtttg cagccccaac aacccgaccg ggcaactgat caatccgcag
     7981 gattttcgca ctctgctgga gttaacccgc ggtaaagcga ttgtggttgc cgatgaagcc
     8041 tatatcgagt tttgcccgca ggcatcgctg gctggctggc tggcggaata tccgcacctg
     8101 gctattttgc gcacactgtc gaaagctttt gctctggcgg gccttcgttg cggatttacg
     8161 ctggcaaacg aagaagtcat caacctgctg atgaaagtga ttgcccccta cccgctctcg
     8221 acgccggttg ccgacattgc ggcccaggcg ttaagcccgc aggggatcgt cgccatgcgt
     8281 gagcgggtgg cgcaaattat tgcagaacgc gaatgcctga ttgccgcact gaaagagatc
     8341 ccctgcgtgg agcaggtttt cgactctgaa accaactaca ttctggcgcg ctttaaagcc
     8401 tccagtgcag tgtttaaatc tttgtgggat cagggcatta tcttacgtga tcagaataaa
     8461 caaccctctt taagcggctg cctgcgaatt accgtcggaa cccgtgaaga aagccagcgc
     8521 gtcattgacg ccttacgtgc ggagcaagtt taatgagtca gaagtatctt tttatcgatc
     8581 gcgatggaac cctgattagc gaaccgccga gtgattttca ggtggatcgt tttgacaaac
     8641 tcgcctttga accgggcgtg atcccggaac tgctgaagct gcaaaaagcg ggctacaaac
     8701 tggtgatgat cactaatcag gatggtcttg gaacacaaag tttcccgcag gcggattttg
     8761 atggcccgca caacctgatg atgcagatct tcacctcgca aggcgtgcag tttgatgaag
     8821 tgctgatttg cccgcacctg cccgccgatg aatgcgactg ccgtaagccg aaagtaaaac
     8881 tggtggagcg ttatctcgct gagcaagcga tagatcgcgc caacagttat gtgattggcg
     8941 atcgcgcgac cgatattcaa ctagcagaaa acatgggcat tactggttta cgctacgacc
     9001 gcgaaaccct gaactggccg atgattggcg agcaacttac caaacgagac cgttacgccc
     9061 atgtagtgcg caacaccaaa gagacgcaga ttgacgttca ggtgtggctg gatcgcgaag
     9121 gtggcagcaa gattaacacc ggcgttggct tctttgatca tatgctggat cagatcgcca
     9181 cccacggcgg tttccgcatg gaaatcaacg tcaaaggcga cctctatatc gacgatcacc
     9241 acaccgtcga agataccggc ctggcgctgg gtgaagcgtt aaaaattgcc ctcggcgata
     9301 aacgcggtat ttgtcgcttt ggttttgtgc tgccgatgga cgaatgcctt gcccactgcg
     9361 cactggatat ctctggtcgc ccgcacctgg aatataaagc tgaatttacc taccagcgcg
     9421 tgggcgatct cagcaccgag atgatcgagc acttcttccg ttcgctctct tacaccatgg
     9481 gcgtgaccct gcacctgaaa accaaaggta aaaacgatca ccaccgtgta gagagcctgt
     9541 tcaaagcttt tggtcgcacc ctgcgccagg ccatccgcgt ggaaggcgat accctgccct
     9601 cgtcgaaagg agtgctgtaa tgaacgtggt gatccttgat actggctgcg ccaacctgaa
     9661 ctcggtgaag tctgccattg cgcgccacgg ttatgaaccc aaagtcagcc gtgacccgga
     9721 cgtcgtgttg ctggccgata aactattttt acccggcgtt ggcaccgcac aagcagcgat
     9781 ggatcaggtg cgtgagcgcg agctgtttga tctcatcaaa gcctgtaccc aaccggtgct
     9841 aggcatctgc ttagggatgc aactgctggg gcggcgcagc gaagagagca acggcgtcga
     9901 tttgctgggc atcatcgacg aagacgtgcc gaaaatgacc gactttggtc taccgctgcc
     9961 ccatatgggc tggaaccgcg tttacccgca ggcaggcaac cgcctgtttc aggggattga
    10021 agacggcgcg tacttttact ttgttcacag ctacgcaatg ccagtcaatc cgtggaccat
    10081 cgcccagtgt aattacggcg aaccgttcac cgcggcggta caaaaagata acttctacgg
    10141 cgtgcagttc cacccggagc gttctggtgc cgctggcgct aagttgctga aaaacttcct
    10201 ggagatgtga tgattattcc ggcattagat ttaatcgacg gcaccgtggt gcgtctccat
    10261 cagggcgatt acggcaaaca gcgcgattac ggtaacgacc cgctgccgcg cttacaggat
    10321 tacgccacgc agggtgccga agtgttgcat ctggtggatc tgaccggggc aaaagatcca
    10381 gcaaaacgtc aaatcccgtt gattaaaacc ctggtcgcgg gcgttaacgt tccggtgcag
    10441 gttggcggcg gcgtgcgtag cgaagaagac gtggcggcgt tactggaagc gggcgttgcg
    10501 cgcgtggtgg tcggctccac cgcggtgaaa tcaccagaaa tggtgaaagg ctggtttgaa
    10561 cgtttcggtg ccgatgcctt agtgctggcg ctggatgtcc gtattgacga gcaaggcaac
    10621 aagcaggtgg cagtcagcgg ctggcaagag aactcgggcg tttcactgga acaactggtg
    10681 gaaacctatc tgcccgtcgg cctgaaacat gtgctgtgta ccgatatctc gcgcgacggc
    10741 acgctggcag gttctaacgt ctctttatat gaagaagtgt gcgccagata tccgcaggtg
    10801 gcatttcagt catccggcgg cattggcgac attaacgatg ttgctgccct gcgtggcact
    10861 ggcgtgcgcg gcgtaatagt tggtcgggca ttattggaag gtaaattcac cgtgaaggag
    10921 gccatcgcat gctggcaaaa cgcataattc cgtgtctcga cgttcgtgat ggtcaggtgg
    10981 tgaaaggcgt acagtttcgc aaccatgaaa tcattggcga tatcgtgccg ctggcaaaac
    11041 gctacgccga agaaggtgca gacgaactgg tgttctacga tatcaccgct tccagcgatg
    11101 gccgtgtggt agataaaagc tgggtatctc gcgtggcgga agtgatcgac attccgttct
    11161 gcgtggcggg tgggattaag tctctggaag atgccgcgaa aattctttcc tttggcgcgg
    11221 ataaaatttc catcaactcg cctgcgctgg cggatccgac gttaattact cgcctggcgg
    11281 atcgttttgg cgtgcagtgt attgtggtgg gtattgatac ctggtacgac gctgaaaccg
    11341 gtaaatatca tgtgaatcaa tataccggcg atgaaagccg cacccgcgtc actcagtggg
    11401 aaacgctcga ctgggtacag gaagtgcaaa aacgcggtgc cggagaagtc gtcctcaaca
    11461 tgatgaatca ggacggcgtg cgtaacggtt acgacctcga acaactgaaa aaagtgcgtg
    11521 aagtttgcca cgtcccgcta attgcctccg gtggcgcggg caccatggaa cacttcctcg
    11581 aagccttccg tgatgccgac gttgacggcg cgctggcagc ttccgtattc cacaaacaaa
    11641 taatcaatat tggtgaatta aaagcgtacc tggcaacaca gggcgtggag atcaggatat
    11701 gttaacagaa caacaacgtc gcgaactgga ctgggaaaaa accgacggac ttatgccggt
    11761 gattgtgcaa cacgcggtat ccggcgaagt gttaatgctg ggctatatga acccggaagc
    11821 cttagacaaa accatagaaa gcggcaaagt cgccttcttc tcgcgcacta aacagcgact
    11881 gtggaccaaa ggcgaaacgt cgggcaattt cctcaatgtg gtgagtattg caccggactg
    11941 cgacaacgac acgttactgt tactggtgaa tcctatcgga ccaacctgcc ataaaggcac
    12001 cagcagctgc ttcggcgaca ccgctcacca gtggctgttc ctgtatcaac tggaacaact
    12061 gctcgccgaa cgcaaatctg ccgacccgga aacctcctac accgccaaac tgtatgccag
    12121 cggcaccaaa cgcattgcgc agaaagtggg cgaagaaggc gtggaaaccg cactggcagc
    12181 gacagtacat gaccgctttg agctcaccaa cgaggcgtct gatttgatgt atcacctgct
    12241 ggtgctgttg caggatcagg atctggactt aacgacggtg attgagaacc tgcgtaaacg
    12301 gcatcagtga gttgcggggt aagcggatgc gatattgttg tagcatccgg aaaaaaaaac
    12361 gggcaaggtg tcaccaccct gccccttttc tttaaaaccg aaaagattac ttcgcgttgt
    12421 aattacgcag agcgttacgc cccaagacaa tccccgcgcc aaccatgccg cccagcagca
    12481 ccgccagaat caaggtaatt gcctttttcg ggctatcgcg acgaataggt aacgtcggtt
    12541 tcattacata gcggtaagca tgaatatcaa gtttatcaac gtcaagattg tcgatatcca
    12601 gcaggttttg acgagtctga tagtagtttg gtgagaacac caacggacga gtcgcttcat
    12661 gcttaatcat cgactccagc gcttcgctcc ccaaaaggaa caacgtatct tgtgtcacat
    12721 cttcagtctg ctgaacctgt ggctttgtca cctgcgcctg attcgcatac tgcaacgctt
    12781 cctgaatctg acggatacgc agatctttct gctcctgcgc aactacttcc tgagttttca
    12841 gagaatcctg caagtttttc atacgcagcg cgatgttgtc tttcaggtct tttcccagtt
    12901 cttcatttac ctggtcgtca acttgctgga tatacttcgc cagctgcttc tgagcacctt
    12961 caggtgtttc accaacataa gacaccgcta aaggtaatga ctgatttttg acggtaggct
    13021 caatggtcag cttttctgct tcttcctggt tatccagcgt ttccgctaat gctgagaatg
    13081 cagtgctgta acgtccgata agtgaagcct gaatgtcgga gactttcggt atagccgatc
    13141 cgtagatcac attcatcgcg ttggtataac cagcgatttg ccccacatca ggttgagtaa
    13201 cgatagctgt ggaagcccac ttttctttgg caacggccag ataccctata gccagggcaa
    13261 tagcaacaat gacagagatg ataattgtca tcttgccacg ccacaactgc accagtaaat
    13321 caatcaaatc aatctgttcc ggatcatggt tttgcccaga aacattatta ttttctactc
    13381 tcatcattac cctaacggac gaaataattt taagtcgcca tagtttagcg ataatcgcca
    13441 tgaatgctat aggataaatg ataaaaataa tgaattatta caaagggaac ataagagaat
    13501 cagggcgaaa atcgccatga taacaggatg ttagtcactg ccaaagagat cgcgggtgta
    13561 gactttgtct gccacatcct taagctcttc tgccatacgg ttggagataa tgacgtcggc
    13621 ttgtatagtc agcttagaac agctttgatt gtaaaatgag cacgttacca gaaaaaacag
    13681 ccaagtttag aactgatacc gattatcttt ttttcttgtc taacggtata atttaacttt
    13741 cagttatgcc agatgaagat tggctatatt ctagcctgag cgaggattat aaatgaaatt
    13801 tctggttacg ggagctgctg gctttatcgg tttctatcta agtaaacggc ttcttgcagc
    13861 tggtcatcag gttgtaggca ttgacaactt aaatgattat tacgatgtca acctcaaaca
    13921 agcacgactt gatttactca agcacgacaa cttcagtttt tataaaattg acctggccga
    13981 tcgtgagaaa atggcggcac tgtttgcaga cgagcggttc gaacgcgtaa taaacctcgc
    14041 tgcgcaagcc ggtgtacgtt actctcttga aaatcccaat gcatatgcag atgcaaacct
    14101 gattggattc ctgaacatac tagaaggatg tcgccataat aatgttcagc atctacttta
    14161 cgcttcctcc agttctgttt atggcatgaa ccgcaagatg cctttctcta cagatgactc
    14221 tgtagatcat cctgtttcgc tttatgcagc aactaaaaaa gcgaatgaac tcatggccca
    14281 tacatattct catttgtatg gcttaccgac cacagggctg cgtttcttta cggtttatgg
    14341 tccatgggga cgtccggata tggcattatt taaattcact aaagccatgc tggaaggtaa
    14401 aagcattgat gtttacaact tcggcaaaat gaagcgtgac tttacttaca ttgatgatat
    14461 tgccgaagct attattcgct tacaggatgt tattccagaa aaaaacccac agtgggctgt
    14521 agaaacaggc tcgcctgcaa caagttcagc accatatcgt gtctataaca ttggtaatag
    14581 ttcgcctgtg gagttgatgg actatatcaa tgcgttagaa gaggctctgg gtattgaagc
    14641 caacaaaaat atgatgcctc tccaacccgg tgatgtactg gaaaccagtg ctgatacaaa
    14701 agcactgtat gacgtaatag gattcaaacc tgaaacgtca gttaaagaag gggtaaagaa
    14761 ctttgtagaa tggtatcgta acttctataa agtttgattt tacaaaacca taagaaaagg
    14821 ccctaattta ttagggcctt ttcttagaat gaaacaaaat aattaatcat tgccaaacaa
    14881 gtcgcgcgta taaactttat ctgctacatc agccagatcg gcagacatac ggttagaaat
    14941 aataacatca gcttcttgtt tgaacgcatc cagatcacgt accacgcgcg accggaaaaa
    15001 atcgtcctct ttcatagctg gctcataaac gattacaggc acacctttcg ccttgattcg
    15061 cttcataata ccctgaatcg aggaagcacg aaaattgtct gaaccattct tcataatcaa
    15121 acgatagacg ccaacaactt tcggtttacg tgcaaggata gaatcggcaa taaaatcttt
    15181 gcgcgtgcgg ttggcgtcaa caattgccga gatcaggtta ttcggcacag actggtaatt
    15241 tgccagtaac tgcttagtat ctttcggcag acaataacca ccataaccga atgacgggtt
    15301 gttgtagtga ttaccgatac gcgggtcaag gcatacgccc tcaataatct ggcgtgaatt
    15361 aagtcccagg ctttcagcat aactatcaag ttcattgaaa tacgctacac gcatcgccag
    15421 ataagtgttc gcaaaaagtt taatcgcctc agcctcggtt gagtcagtaa acaatgttgg
    15481 tatgtcttgc ttaatggcgc cttcctgtaa taacgcagca aaacgtttag cgcgttcaga
    15541 ctgctcgcca atcacaatgc gtgatgggtg taagttatca taaagtgctt taccttcacg
    15601 caaaaactca ggcgaaaaga tcacattttc aataccaaaa cgttctttaa tggactctgt
    15661 aaaaccaaca gggatagttg attttataat cattaccgcg ttgggattaa tttctgtcac
    15721 atcacgaatg accgcttcca cgcttgaggt attaaaataa tttgttttcg gatcataatc
    15781 ggtaggtgtg gcaataataa cgtaatcggc atttttatac gcgtcatact tatctgtcgt
    15841 agcgcggaaa ttgagatctt tagtcgccag atactcttca atctccttat caacaagcgg
    15901 tgactgcctc ttgttaagca tgtccacttt ggcctgaacg atatccagtg caaccacttc
    15961 gtggttttgc gcaatcagaa taccatttga aagaccaaca taacctgttc ctgaaattgt
    16021 tattttcatt agctctgact tcttccggtt aaacatttag agtggtcatt aatccaccac
    16081 cacagcttca tctactgcgg gatttttaac gctgatgtag atgaactgtc aaggcagcga
    16141 tcctgctgtg cggcgctgta ttatatcgcg tttttaacta taaattataa aaaaaggccc
    16201 taacctgccg ctttgtataa taaaaaagcc cggagggttt ctccgggcct tgctttgatt
    16261 aattgattta aatcagatta atccagccat tcggtatgga acacaccttc tttatcaatg
    16321 cgcttataag tatgcgcacc gaaatagtca cgctgtgcct ggatcaggtt cgcaggcaga
    16381 acagcggcac ggtagctgtc ataataggca accgcagcgg cgaaggtcgg aaccgggata
    16441 ccgttctgta ctgcataagc aacgacatca cgcagcgcct gctggtaatc atcggcaatt
    16501 tgtttaaagt acggagccag cagcaggtta gcgatctgcg gattttcggc atatgcatca
    16561 gtgatttttt gcaggaactg cgcacggatg atgcagccag cacggaaaat cttcgcgatt
    16621 tcgccgtagt tcagatccca gttgtactct tcagacgcag cacgcagctg agagaagccc
    16681 tgggcgtaag aaacgatttt gcccagatac agagcacgac gaactttttc gatgaactca
    16741 gccttgtcgc cagctggctg cgcttgcggg ccagagagaa ctttagatgc ggcaacacgc
    16801 tgatctttca gagaagagat ataacgtgca aacacagact cggtaatcag cgacagcggt
    16861 tcgccgagat ccagcgcact ctggctggtc catttacccg tacctttgtt agccgcttca
    16921 tccaggatca catcaaccag gtagttaccg tcttcatctt ttttggtgaa gatatctttg
    16981 gtgatgtcga tcaggtagct gctcagttca ccgttattcc actcggtaaa ggtctgcgcc
    17041 agttcttcgt tggtgaggtt caggccacct ttaagcagag aataggcttc agcaatcagc
    17101 tgcatatcgc cgtattcaat accgttgtga accatcttca cataatggcc tgcaccatcg
    17161 gcaccgatat aggtaacaca tggctcaccg tcttcagcca ctgcggcgat tttagtcagg
    17221 atcggcgcaa ccagttcata ggcttctttc tggccaccag gcataatgga aggccctttc
    17281 agcgcgccct cttcaccacc ggaaacaccg gtaccaatga agttaaagcc ttcagcagaa
    17341 agctcacgat tacgacgaat ggtgtcctgg aagaaggtgt taccaccatc aatgatgatg
    17401 tcacctttat cgaggtatgg cttgagggaa tcaatagcag catccgtgcc tgcacctgct
    17461 ttcaccatta acaggatgcg acgaggcgtt tccagagatt caacaaactc tttcaccgta
    17521 tagtaaggaa ccagtttttt gcctggattt tcggcaatca cttcttccgt cttttcacgg
    17581 gaacggttga aaatagagac ggtataacca cggctttcga tattgagcgc aaggttgcgc
    17641 cccatcactg ccataccgac gactccgatc tgttgctttg acattgttta ctcctgtcag
    17701 ggggtgttca ccacgggttt gcgcggcttg aatcaagttt gaagagtacc ttataacctc
    17761 aactttacct acctgaaata tctatatgtt cttaatattc ttactccatt tttacaaaag
    17821 ctgctcatac tgttcacgaa ttctattcca tgtgtaatgt ttttcagcca gcgacttcaa
    17881 cttaacacct tgtgttctct ctttgggtga ttctatttta attataagat ccttcaggtc
    17941 tctgaaataa cacgcttgtt caaatgttgt ataacgatta tacttacaat caaaacaaat
    18001 aatcggcttt gcaaaaaaca tcatttcaac tagtgatgga ttagtacctc cagcagaatg
    18061 tccatgcaca tagaactcac atccgcttct aataatattt aattgttcaa catcataaat
    18121 tggatcaata atttctatat tattgtactt tgaatatttt ttcttaagac tacgcccata
    18181 gtcgctactt ccccaattac caataaactt caattttttg gtgactttag aaaaagattc
    18241 taatataaca tgaacattgt tttctggctc aatacgacag agagcaagag catatttact
    18301 ttcggaattc tccacatcgt tacatctttt tgtttcaaaa actagtgaat gatctccgcc
    18361 gtaggcaatt acttgagaat ttaccatata ttctgagcac acatagtttg cgatttcctt
    18421 attatcagaa atcacaatat tagaattttt cacagctatt ttttcagacc attttaaaaa
    18481 ttttttagca aacctacccc atttatctcg acgccattct actccatcaa tgtttgtaat
    18541 tattttaaca gatgatattt tttttattat aggaagaata aagcaaccag agactcctaa
    18601 aataaggagt gtatttactt ttcggaatac aatcgcatgc aatatgctaa atgcgtcata
    18661 aataacactt gatattccat tagcgctaag aggaatataa accaatttcg catttttata
    18721 tgtgcttagt tttttggggt aattttttga tgagcaataa acaatataat taaccttttc
    18781 agtttcaatt aaattttcaa caagtgtttc aaaccctcca tacgaagcag gaacacctac
    18841 agtaccaata atggcggtat tactcatatg tttgttcatt tttttcataa atactacttg
    18901 atttattatt cacagttaca catttcatta atacaagaat tagaaatacg ggttcaaaaa
    18961 atagattatt aaggagtgcc aaggtgaaac ttacaagaat aagaagtaag catatattta
    19021 tttcatctac tgaaacaaaa ttatggtttc gtcttttctt taagttttta gagagagaaa
    19081 taaacatgta actccaagta gcgatatata aaacaagagc tataagacca ctctgatgca
    19141 aaagatataa ataaccatta tcgacgtaaa aattcatatc atcttcccct aacccctgca
    19201 ctaaaccgga accaaataat aaacgagaga cgtcattaat attcaaaacc tgaagccagg
    19261 catctatacg agagaataat gtactcaatt ttgtaaaagc tgaataatct atagaagaat
    19321 atgaattctg aatgtcacca ccaaagtata atataaaact tggtgctatg aacaatattc
    19381 caatcaaaaa aacataatat aaattcgcaa gagttagata gtatcgacga aggtaagtat
    19441 gaatcattat aaataaagat gtaactgcaa atgccaacat cccgtttcta ttatatgtca
    19501 aaacaaccat tatgacaaaa ccaacaaata gaaaagcaaa ctttatacga ttgacagatt
    19561 tattaaaata ataataaatt attgcaaagc aacaataact agctaagata aacccagatg
    19621 aaaatatccc actaaaacga agagtaatac cttcttcact tgtttgaaat ggattaatta
    19681 ttaaaccatg attgtataat tgcgaataaa actcttcaac cattttagag tcatacatca
    19741 cacataaccc aaatattaaa agtgataaaa aggtaagctt tgttattata ttaatcaaag
    19801 agtcaaaaat atccaatttc aactttcccg aaccggctaa acaaatgatt ataattggaa
    19861 aaatatattg tatcgttact gcaagcatta attgtaattg tgtataaaca ttattaacaa
    19921 cgatgttatt tacaagaaac aaaattgcag atatggaaaa gaaaaatata aaacatgccc
    19981 cttgagcttt catttttatt gttctgttaa ttaacagata aaacagagag gctaatatta
    20041 atagaggaat aaacacataa ttaagattaa tatttatata caatgatata gccttaatta
    20101 atccagacaa taaaaaaaac agcaataaca actttggtgc ccaagctagc ataaattaat
    20161 tcctcttgtt attatatcta gccttaaagt aactatcaat tttcttgaaa tttatatcga
    20221 gatctagttg tattctgact tttttactac tttcaatagc aaaaagattt agttcttcat
    20281 ccttttgtga aagcttcata atagcaacca tgaaggaatt tagcgttctt tccttgagaa
    20341 caaagccagt cacttcattt ttgataaatt gttctacgcc accatttctc gttgtaacta
    20401 ctattgcccc actaaaagat gcctctgctg cagtcaaacc aaatccttca tatatactac
    20461 tgcaaatgaa gtatctattc tcattatata aattatttag ctgtttaact gtgggttgat
    20521 atacgtaatg aataaacgaa gggaaatttt ctggttttgt ataagcacta aagcaactga
    20581 atttaattga gctatcaact tcgtgaattt tttgtattgc tcttaataag agatcacaac
    20641 cctttctttc actaggatga tatagaaata aaaatgaagc tctatccctt tcattccatg
    20701 gaatgttaac aaaaaaatca tttttgttta ttccattgta caaggtgaga gaaacatccc
    20761 ctcccatttt tgtaatcttt ttcaccaagt catcagaaat agttatataa tcaacatctt
    20821 tatgtgaaag tgtatagttt aaaatattct caggaatatt ccagttttca taatcctgta
    20881 caaaatatac taatttttta tggttaactc tacatgacga attggcgtat ataaacgtct
    20941 caacagagga gcaaatgaca atattaaaat tttcgatatc tctttttttt agagatttaa
    21001 tcacataatg atttatatta gaggatgtaa aatcaaacca tttgtagaac tttaataatg
    21061 aaaataattt tgcttttatt ttacccacaa ggccagaaaa tcgattataa caaacaatac
    21121 ggttcgtcaa ataaataaaa tcaatctgcc atccaacaga tgccaaatag tttccatatt
    21181 gatacaagac tttatatcca ccaacaggct cccttcttgg tcctggtagt aaaatagcta
    21241 tttttttcat ttagacctta aatttataac cacatttttt agccaacata tacatacata
    21301 tcaaaattga taattgtatg cataaggatg ttaatattgt gccatgcagg tgatacagtt
    21361 tactaaatac aggcatggct ataatattta aaataccatt gattgcaata attttagtga
    21421 agcttttgtc tttcccataa ttcaatccaa tttgtgtacc aaagagattg ttttggaata
    21481 cgaagaatac attaaataac aacaataata atacagagta taattcttgt gatattgttg
    21541 gaaaaataag ctgtatgaga taatagccta taaaaacata caacgtgtaa cataacagaa
    21601 aaacaattaa actaaataat gatattcgag aaatcattat aaatactctt tgcttattat
    21661 tttcaattaa tgatgaaata tgtggaaaaa taacgtttag aataggcgtt gttgtaatcg
    21721 ataacacctt cacgattctt tcagcagttg aaaagacaac gaaactttta ggttccaaca
    21781 ccaaaggtag aaagaaaata ttggcataag aaatcaatgt cacactagct tgagatagga
    21841 atatatacca ccctttaact aaaaatatcc atacatttct gagcaaatta gtacgtttaa
    21901 aagctactac attcaacttc ctcctaatat gtatcatcat tagtatgttt gatgcataat
    21961 ttgctattgc acataaaaat agataaaccc aagcatcatt atcattattg accaaaataa
    22021 aaacgaaggg ataaacaata agcttaggaa gtaagccata gaacgtaatt aacttaaaat
    22081 caccacatcc ttgcaaatac caatttgatt gaaatacaaa gcttagcatc caaaaaaaac
    22141 atagcaacca ataaaggagg tcgggtttag tcaatgccat caacgacaca agcaaaaaga
    22201 atgagcaaaa tataaaaata cagagccgtg acaatatgga aatataaaga aaactcttac
    22261 attttcttag atgctttaca ttattactaa tatatctaac accaaacaga tccagcccat
    22321 aattaattaa aacagcaaaa aataatacta atgattgaat aacaacaaaa tctttataga
    22381 ccgcaataga gagtttattg attaagaaag gaattataag aaagggtaga atataattcc
    22441 caaactggta aataaattga ttaactattt tttttaccac attctttcct tcgaataata
    22501 tcttcgataa ttttattaac ttttatttca aataaccatt ctttttgtat gctggcttta
    22561 atgctttccg aattatttga gacaattaat tcatttattt tctcatataa caaatctctt
    22621 tttgagtatt ctagataatt aacaaaccat cgactcttaa tttcaaaatt catagatgat
    22681 aaacatggaa tgccatattt aaagtactca tacattttca atggtgattc atcatgcgat
    22741 ttttctggca gatatggcaa taatccgact ttaaaagatt tcattattga aggaagatca
    22801 gagtatttaa atgcccccgc tatttttata ttttcattca gcttatcacg tatagaatca
    22861 tcaaaaaatt ttccccaaat gatgatactg aatttttttg ataatttatt taaaaaa
//
LOCUS       DADLFA010000052        22864 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000052 DADLFA010000000
VERSION     DADLFA010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22864
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.052"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            39..968
                     /gene="aaeR"
                     /locus_tag="JQH65_001703"
     CDS             39..968
                     /gene="aaeR"
                     /locus_tag="JQH65_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HAY6139153.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            complement(1096..2541)
                     /gene="tldD"
                     /locus_tag="JQH65_001704"
     CDS             complement(1096..2541)
                     /gene="tldD"
                     /locus_tag="JQH65_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HAY6139154.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVGHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVRGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(2697..6497)
                     /locus_tag="JQH65_001705"
     CDS             complement(2697..6497)
                     /locus_tag="JQH65_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein"
                     /protein_id="HAY6139155.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGGNDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFHAPLEIAD
                     GQATISWLNNDKGFHLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDDVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKLLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPAAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLPVNVKVDGNLSSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDLTISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            complement(6565..8034)
                     /gene="rng"
                     /locus_tag="JQH65_001706"
     CDS             complement(6565..8034)
                     /gene="rng"
                     /locus_tag="JQH65_001706"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HAY6139156.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            complement(8024..8617)
                     /gene="yhdE"
                     /locus_tag="JQH65_001707"
     CDS             complement(8024..8617)
                     /gene="yhdE"
                     /locus_tag="JQH65_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HAY6139157.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAHKKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVVLADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            complement(8626..9114)
                     /gene="mreD"
                     /locus_tag="JQH65_001708"
     CDS             complement(8626..9114)
                     /gene="mreD"
                     /locus_tag="JQH65_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAY6139158.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            complement(9114..10199)
                     /gene="mreC"
                     /locus_tag="JQH65_001709"
     CDS             complement(9114..10199)
                     /gene="mreC"
                     /locus_tag="JQH65_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAY6139159.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPTTGIAQPTPQQPTTGNAATA
                     PAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            complement(10265..11308)
                     /locus_tag="JQH65_001710"
     CDS             complement(10265..11308)
                     /locus_tag="JQH65_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HAY6139160.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNN
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            complement(11613..13553)
                     /gene="csrD"
                     /locus_tag="JQH65_001711"
     CDS             complement(11613..13553)
                     /gene="csrD"
                     /locus_tag="JQH65_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HAY6139161.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKYPGMSLRLVYQDPMGNYFHSLMTTAPLTGAISFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            13705..14679
                     /locus_tag="JQH65_001712"
     CDS             13705..14679
                     /locus_tag="JQH65_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY6139162.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVMVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAIIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            15657..16127
                     /gene="accB"
                     /locus_tag="JQH65_001713"
                     /gene_synonym="fabE"
     CDS             15657..16127
                     /gene="accB"
                     /locus_tag="JQH65_001713"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAY6139163.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            16138..17487
                     /gene="accC"
                     /locus_tag="JQH65_001714"
     CDS             16138..17487
                     /gene="accC"
                     /locus_tag="JQH65_001714"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAY6139164.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(17579..18626)
                     /locus_tag="JQH65_001715"
                     /pseudo
     CDS             complement(17579..18626)
                     /locus_tag="JQH65_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787050.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
     gene            complement(18623..19585)
                     /locus_tag="JQH65_001716"
     CDS             complement(18623..19585)
                     /locus_tag="JQH65_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAY6139165.1"
                     /translation="MSKVFTIGEILVEIMASKIGQPFDQPGIWNGPYPSGAPAIFIDQ
                     VTRLGVPCGIISCVGNDGFGDINIHRLAADGVDIRGISVLPQEATGSAFVTYHNSGDR
                     DFIFNIKNAACGKLSAQHVDENILKDCTHFHIMGSSLFSFHMVDAVKKAVTIVKANGG
                     VISFDPNIRKEMLDIPEMRDALHFVLELTDIYMPSEGEVLLLSPHSTPERAIAGFLEE
                     GVKEVIVKRGNQGASYYSANEQFHVESYPVEEVDPTGAGDCFGGAWIACRQLGFDVHR
                     ALQYANACGALAVTRRGPMEGTSRLAEIEAFIQRHDMSIREAAQ"
     gene            complement(19607..20596)
                     /locus_tag="JQH65_001717"
                     /pseudo
     CDS             complement(19607..20596)
                     /locus_tag="JQH65_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525780.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(20597..22096)
                     /locus_tag="JQH65_001718"
     CDS             complement(20597..22096)
                     /locus_tag="JQH65_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAY6139166.1"
                     /translation="MTDVILDVSHIAKTFGHVQALKDITLSLRKGRVHTLLGENGAGK
                     STLMKILAGVYPPTQGTITLRGETITINNPQHSRQLGIAIIFQELSLSNNMTVAENIY
                     ANNEPRRFGIINDKKMLADCQNLLAERGIPLDPLEMVGNMSMAHRQLVEIAKALSYAA
                     DVVIMDEPTSSLSDNEAEILFNIIEKLKQRGCAVIYISHRMDEIMRISDDISVIRDGE
                     YIATHEKKNSDIQHLIAQMVGREMKNIWPARLGEIPDENVPAKLEVKNLSHPSLFKEI
                     SFAVRPGEVLGFFGLVGAGRSDVMKALFGLVSYQGTVLIDGKEVRITNPKQAIDHGIA
                     FVTENRKEEGLVLMHDVNMNTHHVAFQYNASRMGLINHRQEEAKTLQSIARMNTKVSS
                     VHQAVGALSGGNQQKIVLSKWLEKTPRILLLDEPTRGVDVGAKFEIYNVIRQLAAAGT
                     AIILVSSELPEVMALSDRLVVMRNKTIADIYSCENLTQTQVMTAATGVR"
     gene            complement(22157..>22864)
                     /locus_tag="JQH65_001719"
     CDS             complement(22157..>22864)
                     /locus_tag="JQH65_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAY6139167.1"
                     /translation="LFANQDLNKQLSDVEDFITKKVDAIIISPVDSKGVQAAIIKADK
                     AGIPVITVDVAAEGVPVVSHVATDNYAGGVEAGKLMGKLLNGKGTVASISYPALQSVV
                     ARVDGFKKGLSDTPDIKIVAEQPSITRAEALTTAQNIMQANANLNGLFGFGDDAALAA
                     VIAAKSAHNDNIKIIGFDGMKEARDAVDSEKTFAAVIRQYPDQMGAKAIDAAVDHLNG
                     KPVEKMIPVQPGVYTGK"
BASE COUNT         5668 a         6192 c         5459 g         5545 t
ORIGIN      
        1 attatgtgat ctaaatcact tttaagtcag agtgaataat ggaacgacta aaacgcatgt
       61 cggtgtttgc caaagtagtt gaatttggct cttttaccgc cgccgccaga cagctacaga
      121 tgagcgtttc gtccatcagt cagacggtgt caaaactgga agatgagttg caggtaaagc
      181 tgttaaaccg tagcacacgc agcattggcc tgaccgaagc cggtagaatt tactaccagg
      241 gctgccgtcg tatgcttcat gaagtgcagg atgttcatga gcaactgtat gccttcaata
      301 acacccccat cgggacgcta cgcattggct gttcttcaac tatggcacaa aatgttctcg
      361 ccgggctgac agccaaaatg ctgaaagaat acccaggttt gagcgtcaat ctggttaccg
      421 gaattccagc ccccgacctg attgccgacg gtctggatgt ggtgatccgc gtcggcgcgt
      481 tgcaggattc cagcctgttt tcccgccgtc ttggcgcgat gccaatggtg gtgtgcgccg
      541 cgaaaagcta tctcacacaa tacggcatac cggaaaaacc cgccgattta agtagccatt
      601 catggcttga atacagcgtg cggcccgaca atgaatttga actgattgca ccggaaggga
      661 tctcgactcg cctgatcccg caagggagat ttgtgactaa tgatccgatg acgctggtgc
      721 gctggctaac ggcgggtgcc gggatcgcct acgtgccgct gatgtgggtg atcaacgaga
      781 tcaatcgtgg ggagctggag atcctgctgc cgcgttatca gtcagatcca cgcccggttt
      841 atgcgttata taccgaaaaa gataagctgc cgctgaaggt acaggtcgtg atcaactcgc
      901 tgacggatta ttttgttgag gtcggtaaat tgtttcagga gatgcacggg cgcgggaaag
      961 agaagtaatt catattgtac tgttacgttc tacaaacctg tgccaacggg ttcccctcac
     1021 cctaacccca gaggagcgag gggaccgttc gtgcacgtag aaagattaat tatccttctg
     1081 aaaataatga aattattacg cagtaccgcc aacagtcagg ttatcaactt tcagcgttgg
     1141 ctggcccacg ccaaccggca aactttgccc ttctttaccg cagacaccca cgccgttatc
     1201 cagtttcagg tcgttgccaa ccatcgaaat ctgctgcatg gtttcgatac cggaaccaat
     1261 caacgttgcg cctctcaccg gcttcgttac tttaccgttt tcaatcagat atgcttctga
     1321 agtggagaaa acgaatttgc cggaggtgat atccacctga ccgccaccaa agttcggtgc
     1381 atagataccg tactcaacgg attcaataat ttcctgcggg gtcgatttac ccggcagcat
     1441 ataggtgttg gtcatacgcg gcatgggcag atgggcgtag gattcacggc gaccgttgcc
     1501 agtcggcgtc atccccatca aacgcgcgtt gagtttatcc tgcatgtagc ctttcagaat
     1561 gccgttctca atcagcacgt tgtactggcc tggcgtacct tcgtcatcaa tcgccaccga
     1621 accacggcga tcgaccatcg tgccgtcatc aaccacggta cacagttctg aagccaccag
     1681 ctccccgacc tgcccactaa atactgaagt gccgcggcgg ttgaagtcgc cttccagacc
     1741 gtgacctacc gcttcatgca acagcacgcc cggccaacct gcgccaagta ctaccggcat
     1801 ggtgcccgct ggtgcagcaa cggcagaaag attgaccagc gccatacgca ctgcttcttt
     1861 tgcccatgca tccgcacgga cttcgccgtc aagatcggca aggaagaact cataaccaaa
     1921 acgaccgcca ccgccactgg caccgcgttc gcgtttgcca tcttcttcga ccagaacgct
     1981 cacggaaaga cgcaccagcg gacggacatc cgccgctagc gtgccgtcgg tggccgcaac
     2041 cagaattaat tcatagacac cactgaggct ggcagtcact tcctgtacgc gcttgtccgc
     2101 ttcgcgagca accttatcga cgcgacgcag gatatccagc ttctcttcac ggctcatgct
     2161 ttgcagcgga tctaccgagg tatacaacgg gctatgccct accgcgccca gcgtctgtac
     2221 tttgccatca ccactatcac ggacgatggt gcgcgccgct tgcgcactct gttccagcgc
     2281 cagcaggctg atttggtcag cgtaagcaaa tccggttttt tcaccgctga ttgcacgcac
     2341 gccaacgccc tgatcgatgt tgtaagaacc atctttaata atgcggtctt ctaaaaccca
     2401 ggattcgtga tagctcgact gaaaatagag atcgccataa tcaaggcgac gttcggccag
     2461 ttgaccgagg atcgcgaaca agtcctgatg tttcaggccg ttcgccgcta gcaattgttc
     2521 acttactagg ttaagactca tcgtttttgc tactcgttag ttactgcagt agaggatttt
     2581 ttacggctgc cggatgcggc gtgaacgccc tatccgacct acggttatgt tctggctcgt
     2641 tggcctttgg caacgattat catatgagat tggggcaatt acgcgccctc gtcaaatcat
     2701 tgcgcttttt ctttacgcgg ttggcgcaac acttcgttga tttgcggatc gtccagcgga
     2761 cccgaaatgt gatagcgcaa aatggagact ttgctccaca gcggccccag cactttactg
     2821 gctgcaaata ctgccgcgcc aacaatgggg ttaaccgcaa aagccgcagc cacgcccacc
     2881 gtcgcagaaa tctctggtgc gacaaccgct tccatattca ggtcgcgacg taccagattt
     2941 accgaccctt tcatggcgat atccgcctcc aggccatcca ccagcgtgtc gtcggtgtgc
     3001 ataacgccgt ctttaatcca cgcggtgctg cgaatggagt caaaatagaa cccttcgcca
     3061 aaagtgtctc tgaaatcaaa acgcagctta cgcatcaggg catcaacgct caataagcgc
     3121 agcaattgcc cggcatgtcc ggtattgatt tcggtaattt cgcctttacc cagttgagta
     3181 tgaatgatgc cattcaacgt cgcctcatct ggttgccagg gtgctttgcg ccagtgtaaa
     3241 tcgtaatcca cattaaatga cgactggcgt atgggcgtcg tgacaccaaa aaattctgcg
     3301 gcggcatcaa ttttctgccc gcgcagtttt cctttcagcg aggtacgttc attccccgga
     3361 ttattaaccc attcaccatc ggcagtaagc cgcgagaaac cagtatcaat cagtccattg
     3421 gtcagcgtta acgtatcgcc agaaatggtg agatcactgt caatacgacc gaatttttgc
     3481 ccccagaacc agcactctgt gcatcgtatt tgtgcgtccg gccagccacg gaagttaatg
     3541 cgctccgttg tcgggaaagg tgatgacggt gttgaatcac cacgagtttt cgccacgctc
     3601 gggttgtaat aaagatattt gatattcgcc agccacggcg cgttattacg catcgccagc
     3661 gtggcattga tttcacgccc ctgtgcctca accagggtgc catttgccgt cggttgcgaa
     3721 acaatactca ggttattcca ttgctgattt cccagtgaca acataggcgt acgtaacgtt
     3781 atgtgttgtg ggaaactcgc tgcaccaccg acactctccg cagcgccttt ctggaacagg
     3841 gccagccact cggcaccatt catcggcggc atattgagtt caacgccact ttgttccggc
     3901 aacggcggga gcgttttact gtctgccgcc caaatagcac gatcgagcgt cagcttttga
     3961 ccaagcaacc agcggctatt gaaatggtta tccgcaccag cctgtccggt taattcaaag
     4021 ctgctgagat tgccatcaac cttaacgttt accggcagtg gttcacccgc aggtttggct
     4081 aacggtgaag gtaagtgact gctcacatta ttcagatcgc cgttcagctc tacgttatag
     4141 gtcgcgccag catgataagg cagatcaatg cccactttac catcccacgc cacgctgcca
     4201 ctcaatgctt cgttcaccgc tgcaggcaga acgccggttt tcgccggttg ccagttaccg
     4261 ttgaggttta ccgcaacctg gtaggctttt gccccttctt tggtggaaaa atccacgttc
     4321 aacggctgat taaaccagct tgctgtcagt ggttcacttt gcagatcgcc attgataaag
     4381 ctgaatttac cgctcaaatt tttcagggtg ctgtcgagta gtttgataaa cagactgtta
     4441 ttacgcagcg tcacttcacc tttcgcggtt accagttcgc cgttcagcgg gatatcaaga
     4501 tgtaagcgag cattcacatc gccgtcgagc tggagttctt gcagggtcgc acccagagaa
     4561 tctttcagcg gtgtctcatc aaagtaaggg ccaacggctt tacccggacc tttaatgtca
     4621 gcgtcaatca gcagtttttc tttcgagtag tcagggatca ctgcggtgag attactcgcg
     4681 cgcacgccgc ccagattaac gtcatcggtt ttcatccata aaccgtcgtt aataaagtcc
     4741 agttcaatat caaggttagt taatgcaggc cagtccggct ggaaggcaaa cttcgcgtta
     4801 cgcagcggca ccagcacttc aaactgacct tcgttgtgtt tataggggaa gagttgcgga
     4861 ttgccaccat aaaccagcgt cgcgttatcc gcttcaccgc cctgaatcgc gccacttaag
     4921 taatcaacta ggtctttacc catcaagttt tccgggaaat agcgccaggc ttgtgaacca
     4981 tcatcggtac tgatgccagc cagaataccc agccagggtt catcgttagc aggttgcagg
     5041 taacgaaaac cgccgcgcgc atggacagct ttggctttaa cgtcaatatt acgcccatcc
     5101 aggtggaaac ctttgtcatt gttcagccag cttatagttg cctggccgtc ggcgatttcc
     5161 agtggcgcat ggaataccgt ttcgtaaggc atctttgcct gcttcatcga cgcggtaagc
     5221 aaaccatttt caacgctgcc ggaaagcgtc ccggagaagt gttccgcacc cggtaataat
     5281 ttccattgct tccaggccag atcgctccac gatgcctgaa aacgggtctt gtctgccgcc
     5341 tgaagcggga tatccagcgc cagagtgtta atcttgccgc tcggttgtgt ggagcgccaa
     5401 acatcaccca gtgcaggtga aagtttcgcg gccagcgggc gtatgccctc caggcctgcc
     5461 agctccagat tactggcgcg aatccggagt tcgtcactgc gtttattgtc tttgccgcca
     5521 acgtcctgtt ccggtatcca ggccagcgtc aatgctccgc tcggccaggg tttgccgtcc
     5581 atcgtgatcc gtgtatcggg aatagagaac tgccagcccg gattttcacg cgtaatatgc
     5641 gcggtcagat tatccaccga cagcgtatgc gtttgcttct cgcccaacca gctggcaccg
     5701 ccctgtttca gccagacgtc accgccggtt acatcgcctt tgtcgatcgt catccagcct
     5761 tcaagggaga actgtgccgt ttccagagca atattgtcct gcatccattt accgagccac
     5821 ggcttcaggt cgatgtcatc cgcctgtagc cagacgcgac cattgcttaa caacccctca
     5881 tcatcgcgca aatccatgcg cacctgcatc acgccgtgct gtccggtaag gctggagagg
     5941 cttaccaggc cttccgcacg gtgtcgacgt ggatcgttca gccaggtgag ttgtgggatc
     6001 gccagctcgg cgcgctgacc ggatggcgtc agaaaactga cttcactgtc gcggagatcg
     6061 aaatggtcaa attgacgaag aaacagatcg ctgatgtgac tggcttccag gctatcatta
     6121 ccaccaccgc tggtgatagg agtgttggtg cgaaagcgca gctgccagaa agtgaggtcg
     6181 cgaaactgcc agcgcatatg taacaagctc tgccagacat ccagcgccag agtaacgcgt
     6241 ttaaccgaaa attcgccgcc atcttttagt tctgcacgga tgtcgtgtgc ttcaagcgtc
     6301 gggccaaaat tctgccagct ggctgagagc tgactggctt ctaccggcat gccagtcgcg
     6361 gattctattt tgttgaggat ttccggacgc caggcgtcaa gatgcggtaa agcaatacgc
     6421 aggccgctaa ccagcagggc tgcgatcaca acgagcgcgg ctccagtaag cagtaaaatc
     6481 cccggcaatc gcctcacccg tcactccttg tctgctaaaa atgtgactca aaaacccttt
     6541 gccggatggc ggcccagcat ctgtttacat cattacgacg tcaaactgct cctggttata
     6601 gagcggttca atttgtactt taacctgttt gccaacgaaa atttccactt ccgccagcga
     6661 gtgtgactct tcgcctttca aggcttcagc tactgccgga gaagcataga ccaggaaacg
     6721 gtcggagtcg taagcatggt ggacacgaac aatctcgcgc atgatttcat agcataccgt
     6781 ttccacggtt ttcaccgttc cgcgaccgtg gcaggttggg cattcgttac acagtacgtg
     6841 ctcaatgctt tcgcgggtgc gtttacgcgt catctccacc agccccagcg ccgaaaaacc
     6901 attaacgctg gttttcaccc ggtctttgct caacgcctgc tccagcgagt gcagcacgcg
     6961 gcggcggtga tcttcattat tcatatcgat gaaatcaata atgataatcc cacccagatt
     7021 acgcaaccgt aactggcgag cgatagcctg cgtcgcttca atattggtat tgaaaatggt
     7081 gtcgtccaga ttgcgatgac cgacaaacgc tccggtattg atgtctacgg tggtcatcgc
     7141 ttcggtctgg tcgataatca gataaccacc ggatttcagt tctactttgc gttccagcgc
     7201 tcgctggatt tcgttttcga catcaaagag atcgaaaatc ggctggcgtc cggtgtaatg
     7261 ctccagcttg ctggtcatct cgggaatgta ctccgaggta aactccagca atgcttcgta
     7321 agtcaggcgt gagtcaacgc gaatgcggtc cagttcggcg tcggcgaaat cacgcagaac
     7381 acgctgcgcc agcgccagtt cgccgtacag ctgataacgg gtctgcgggc gttttttacg
     7441 ctccataact ttggtccaga cgcgtttcag ataagcggca tcggaggcca gttcagcctc
     7501 gccaacccct tccgctgcgg tacggatgat aaacccgccc tgctcgtcgc aatactctgc
     7561 gaccactttt ttcaggcgtt cacgttctga ttcgctttca atacgttggg aaaccccaac
     7621 gtgagaagcc cctggcataa acaccagata gcgagaaggc agcgtgatat cggtggtcag
     7681 gcgcgcacct ttagtgccaa gcggatcttt caccacctgc accatcagat cttgcccctg
     7741 acgaaccagt tccgagatgt cgcgcaccgt gaattgcttt tgttcttcac ccgccacaca
     7801 ttcggtgtgc ggcatgatat cggatgcatg aagaaacgcg gctttatcca gcccaatatc
     7861 tacaaaagcc gcctgcatac ccggaagtac acgacttaca cgacccttgt agatattgcc
     7921 tactattccg cgtcgcgcct cacgttcaat atgaatttcc tgcagaatac cgccatcaat
     7981 atacgccact cgcgtttccg aaggcgttac gtttactaac aattcagccg tcatgtttat
     8041 cccttttctc acgcagtgcg ttaaaattac ttaataattc atacgtttca accagcggta
     8101 agccgactac ggcgtgatag ctgccattta tcttcctgac aaaacagcca cccagcccct
     8161 gaataccgta tgcacctgct ttatctaacg gttcaccgct ggcgacatag cccgcgatgt
     8221 cttcgtctgt taacgttctg aaagtcacat cggtgaccac caggcaatcg agaatgtgct
     8281 ggctgtcggc caacaccact gccgtcatca cctgatgggt ctgacccgat aatttgcgca
     8341 acatctgcgc cgcatgctct gcgtcgcgcg gtttctccag cacttctcca ttcaggataa
     8401 cgatagtatc cgcacccagc accgggagat cctgcgccgt ttgcgcgaca cccgcctgtg
     8461 ctttcttgtg tgccagacgc acaacatact gctgcgcgct ctcctgcggc tgacgctgct
     8521 cctcaatgcc cgtaacaata cgttcaaagg tcacgccaag ttgcgcaagt aactcctgac
     8581 gacgcggaga accggaagct aaatacagag aagtcataga aacctttatt gcactgcaaa
     8641 ctgctgacgg actttacgca tcagcaagaa aatccacggc cagagcaccc cattgactac
     8701 actactccag aacacttccg gtctgaaaga gacgttaatc actaaaaact ctgcccagaa
     8761 aacaataata tccaccacca gcgaaagcaa catgacgact agcgcctgct gccataatgc
     8821 gaggttgcgg aaaagctggt atttcagcgc caccaggtaa gcaatgatgc tcatcgccaa
     8881 tacgcgtacg ccaagcgtcg agccgctgat cagatccagt atggcaccca tcacaaaacc
     8941 tgtgcccaca tttacgcgat ggggcaaggc caggatccaa tacaacaaga taagtaacac
     9001 ccagtttggc cggaaaacaa tcaggttatc cggccagggc atgatttgca gcaacagcgc
     9061 aatgaggaaa gagagccaga ttacccaacg tccctggcta cgatagctcg ccactattgc
     9121 cctcccggcg cacgcgcagg cggttgagca ccactttgcg gcggcgtagc cctttgtgga
     9181 gagcgattag cagcaggcgc agtagctgca tttcctgtcg tcggttgctg cggagtcggc
     9241 tgagcgatcc ccgttgtcgg ttcaggtaac tttggcccca tcgcgtctgg cgaaggcaat
     9301 acctgcggca tcatctgcat cagacgttca ttagcaacac gatgcacctc ttccggcgtc
     9361 atcgggttag cgccgttacg atccgcgccc cacagcagca gcagataacg caaacgttgc
     9421 agtcctgcag tcggacgcgc ctgaatcaca gtataagcgc gctgggtatc gagttttacg
     9481 gaagagacaa ccgcgaccgg atagccttcc gggaaacgac cgcccagacc ggaggtcacc
     9541 agcacatcac caacacgaat atccgtattc gctggcagat gctcaagctg caaatcatct
     9601 gtacaaccgt taccggctgc aattacgcgg atatcgttgc gcagcacctg gattggcagc
     9661 gcgtgggtcg catcacaaat cagcagcacg cgactggtca gtttagcgac ggccaccacc
     9721 tgaccaacaa cacctttgtc gctgatgacc ggctggcctt cataaacgcc attaacgcta
     9781 cctttatcga taacaacttg atcgctataa ggatcgttaa ccgtggagat aacctgagtc
     9841 accattttct gctcatcctg acgcagcggg gaacccagca gctcgcgcag acgcgcgttc
     9901 tcctgtttgt attgtccaag catcagcagt tcactgtttt tcagcaacag ttcctgacgt
     9961 aacgcccggt tttcaagttc tagttggtca cgcgaggcca gcgtctgcga tacgccatcc
    10021 agcaattcac gaggagcatt ggaaacaaag tagaaaggac tgacggcggt atccatataa
    10081 gtacggattt gactgaacgt ccccaggcgg ctgtcggcaa taataatgcc gagcgccacc
    10141 agcaccgcca gaataaggcg aatctgtagc gacgggccac ggctaaaaat tggcttcata
    10201 agttatgcgt attctcgtat cagaccaggc agggtaaaca gacatttccc ctgcctgcat
    10261 ccgattactc ttcgctgaac aggtcgccgc cgtgcatgtc gatcatttcc agcgctttgc
    10321 cgccaccgcg cgccacacag gtcagcgggt cttcagcaac aacgactgga atgccggttt
    10381 cttccattaa caaacggtca aggttacgca gcagtgcgcc accaccggtg agcaccatgc
    10441 cgcgctcgga gatgtcggaa gccagttccg gcgggcactg ttccagtgca accattaccg
    10501 cgctcacaat accggtcagc ggttcctgca gtgcttcgag gatttcgttg gagttcaggg
    10561 taaaaccgcg tggaacacct tctgccaggt tacggccacg aacttcgatt tcacggactt
    10621 catcgcccgg gtaagccgaa ccgatttcgt gcttgatacg ttctgcggtg gcttcaccga
    10681 tcagagaacc gtaattacga cgcacatagt tgatgatagc ttcgtcgaaa cgatcaccac
    10741 caatgcgcac ggaagaggag taaaccacgc cgttcaagga gataacagca acttcagtgg
    10801 taccaccacc gatatcaacc accatagaac cggtcgcttc agaaaccggc aggccagcac
    10861 caattgcggc agccatcggt tcttcaatca ggaagacttc acgggcacca gcgccctgcg
    10921 cggattcacg aattgcgcgg cgttcaacct gggtcgcgcc aaccggtaca caaaccagaa
    10981 cgcgcgggct tggacgcata aagttgttgc tgtgcacttg tttgatgaag tgctggagca
    11041 ttttttcagt cacgaagaag tcggcgataa cgccgtcttt cattgggcga atggcagcaa
    11101 tattgcccgg cgtacggccc agcatctgct tcgcgtcatg acctactgca gctacgcttt
    11161 tcggtgaacc ggcacgatcc tgacgaatgg ccaccacgga aggctcattc aatacgatgc
    11221 cttgtccttt tacataaatg agggtattcg cagtacccag gtcaatggac aagtcattgg
    11281 aaaacatgcc acgaaatttt ttcaacatac taagggataa tcctgaaagc tggggcggaa
    11341 aagaaaatcc gcttacttta ccaaccacac gcagcagcga caaggcgcaa aaatcatctg
    11401 ctacggtgaa aattagtgca gttcgtttcc tttgttacaa atctctgcct gagtccagaa
    11461 aggcttaatg catcagcagc attcctcgcc tgtttgcaac cgcgtaaggt cattcatctg
    11521 catatgtgct gcaacaatct ggcgagcaga caagcacact cccatgagac gcagcgcgcg
    11581 ttattctacg tgaaaacgga ttaaacggca ggttaaaccg agtatctttg tgaatatttt
    11641 ttcacgttag tatcaagtgg ctgtgaggac gcgaaaaaat ccccttgccc gcctgtaaca
    11701 ccgcgctgaa tcagggtctg ccactcgctt cgcgaacgca caccggtggc gtaaacctgg
    11761 gtgctggtcc cggagcaggc ttccaccagg ctttgaacca gcagctggtt ctccgttcgc
    11821 ttctcaatgt ttctgaccag ccccggatgg agcttgagta actcaacatt aagttctttg
    11881 atccaactgg tgcttaccag cgtcaaacca gcctggttga cggctacccg tacccctaaa
    11941 gcattcacta aacgaataac aggttgtaaa cgactgatat gttgacctac atcggcctct
    12001 gcaagttcaa taattatgcg ttttcgttgt gatttttcac attgcattaa cgtatcgcgc
    12061 agccaacgct gaaaacgcgg gcgaatcagc gactcaacgg taacctgaat cgccagattt
    12121 tcctctggcc agtaacgcaa tagtggaata agacggctga tttgcagacg gtcatactct
    12181 tccgataagc caaactgcaa gaccatcggc atatactccg ccgagctaac ctcttcatta
    12241 ccatcgaaga tgcggcacat gagttcgcga tgatgaacct gaccttcgcg agtaaccgcc
    12301 ggtttttgat aaaggcgcgg gccgccgcga ctgagcattt gctcgataag cgtacgccag
    12361 cgaacattac cgcgtccttt ttcaggcaac gagtcatcgt aaatagccca gctattgccg
    12421 ccctgcaatc ccgcattacg cgtggcagac tctgcatgtt ccattacctg ctcggtatcc
    12481 tgaccactac gccaggcgca gataccaatg tggatcatat cgtcgcgatc gagcatttta
    12541 ttgttcggca aggtatcgac ggctttgatt aactgaccgg cgatgctctc tgcctctttt
    12601 aacgtccggt gcggtaacag cgcagcaaaa tcactgcggt ggtaacgcgc cagcagtgcg
    12661 ccagggtagc gcatcataaa tgtcgacagc agattcgtca gagtgaagaa ctgttcttca
    12721 acctggctgt gcccccaagt atcgctcaac atattgaaat ccggcagacg aatcatcatc
    12781 acgatcccgt gggtacctac tttctcctga tcttccagta acgttgctaa ctgattatcg
    12841 aaaaagagtc ggttattgag gccggttttc acgtcctggg cggcataaga gcggatcagc
    12901 gtatcaagac ggctgtgttg ttcgcgtgcg ttctgaattt cacgaagcag cgtatccagc
    12961 gcactgctgg ttctgggcgg ccattcatag atggttccca acacattaga gccacgctca
    13021 ccgtttaaga tacgagtagc ccgggtttcc agcaattctt gcccggcaag ttgccgttgt
    13081 aaccagcgta ccgccaagaa gagcataaca atgataaagc taatcgcccc cgtgagcggc
    13141 gcggtggtca tcaacgaatg gaaatagttg cccatcggat cctgataaac cagacgcagc
    13201 gacatccccg gatactttat caacggaacg ctcagttcac gaaacagatc gctggagcca
    13261 accggacgat aactaccatt tctggccagg gtataaacct gtttatcacc atggagcagg
    13321 tctacacgaa cgatatctgc cgacatcatt aattcggtaa tttgtggcct taatgtgctg
    13381 aagtcattcg acacaaggtg ggtatcaatc gccgtcgcca ccgcctgaac gcgatgacta
    13441 aacttatact gaattgcgtt gtagaaactt agcgaacagc ccagcaaagt cacaaaaatt
    13501 gttaacccgg tgagcagcgt aacaaaggcc gaaaatttcg tcgttaatct catccttgtg
    13561 ttaactccga tagtgaggaa gcgggcatac tagcaaatca gatttatctc gcaatttatt
    13621 gcgcttcatc ggctttgctt ttccattagc gagtataatc ttcagaaatt attttccaat
    13681 ccatcatgca catgaggacc acttatgcag gcgttacttt tagaacagca ggacggcaaa
    13741 actctcgcat cagtacagac tctggacgaa agtcgcctgc cggagggcga tgtcatggtc
    13801 gatgttcact ggtcgagcct gaactataaa gatgcgctgg cgattaccgg taagggaaaa
    13861 atcatccgta attttccgat gattcctggg atcgattttg ccggaactgt acgcaccagc
    13921 gaagatccgc gttttcatgc cggtcaggag gtgttactca ctggctgggg cgttggtgaa
    13981 aaccactggg gtgggctggc ggagcaggcg cgagtgaaag gtgactggct ggttgccatg
    14041 ccgcaagggc tggacgcgcg taaagcaata attatcggta ctgccggttt taccgccatg
    14101 ctgtgtgtga tggcgctgga agatgccggt gttcgcccgc aggacgggga gattgtcgtg
    14161 acgggtgcca gtggtggcgt cggcagtacc gccgtggcgc tgctgcataa gttgggttat
    14221 caggtcgttg ccgtttccgg tcgcgaaagt acccatgaat atctgaaaag tttaggtgcc
    14281 agccgtattc tccctcgtga tgagtttgcc gaatcccgtc ctctggaaaa acaagtctgg
    14341 gctggggcaa ttgacactgt tggcgacaaa gtgctggcaa aagtgctggc gcaaatgaat
    14401 tacggcggct gcgtggcggc ctgtggtctg gcgggtggtt ttactctgcc aaccacggtc
    14461 atgccattta ttctgcgtaa tgtccgtttg caaggggtgg attcagtaat gacgccacca
    14521 gaacgccgcg cacaagcctg gcagcgactg gtcgccgatt taccggaatc attctatacc
    14581 caggcggcaa aagagatatc tctgtcagag gcaccgaact ttgccgaggc catcattaat
    14641 aaccagatcc agggtcgcac gctggtgaag gttaactaac catttagcaa ggaataataa
    14701 gagagggaac tcattttgac atcattattc aatcggttaa cgggaaaagc ggttagccgg
    14761 acagctttcg tcgaacacct tggtcaggaa gttgtacaac atcatccaaa ctggaaagtc
    14821 atgatttcga ctgaccacaa attgatgcgc attgatactc ctctaaacag ccattattga
    14881 tacgcctccg tcgcctgtta ggtttatggt gctttgcctg ggcgacacta cgcttaacct
    14941 gttactgatt tctggtacca tggggtgaat aatctgattt tatttgacta caaattaacc
    15001 actcgaacct atttaatgct gagaattgtc agtcggttga ttttgcgtgc tttagcattc
    15061 acatctatcc agacgatgca gtgaaaattg ggtaatcccc agcaaccgct gcgtaatgtc
    15121 gtctatcttg tcgcgatcct ggcatcccta cattatttgt ggtctgtgaa gattatctca
    15181 ttgcagcccc tcatcttcgc agggctggct ttacagcttt tcaccttacg ttataagaag
    15241 ttccgtcgat gatggcgcta atttcgtgaa ttgtgcggct tgttgcaaat tacacggtgt
    15301 tgaaggttat ttacatgtta gctgttgatt atcttccctg ataagaccag tatttagctg
    15361 ccaattgcta cgaaatcgtt ataatgtgcg acctcgtcct ccctgacgca gtttttgcgc
    15421 tgcggaaaag gtgacattgg cgcaacgaag gtatattttg ttttttgccg gaggatagca
    15481 gcagatcgct gcacaatgtc cgtcaagtct aacattgata ctctggggca aaatagaccg
    15541 gcgtcccggc ctgctggaat ttatcgctat gcatacagct gtcggggcat acgctttaca
    15601 gacggcggtg aaacgcctgt cacaatcaca ctaaacaaag agtacggaac ccactcatgg
    15661 atattcgtaa gattaaaaaa ctgatcgagc tggttgaaga atcaggcatc tccgaactgg
    15721 aaatttctga aggcgaagag tcagtacgca ttagccgtgc agctcctgcc gcaagtttcc
    15781 ctgtgatgca acaagcttac gctgcaccaa tgatgcagca gccagctcaa tctaacgcag
    15841 ccgctccggc gaccgttcct tccatggaag cgccagcagc agcggaaatc agtggtcaca
    15901 tcgtacgctc cccgatggtt ggtactttct accgcacccc aagcccggac gcaaaagcgt
    15961 tcatcgaagt gggtcagaaa gtcaacgtgg gcgataccct gtgcatcgtt gaagccatga
    16021 aaatgatgaa ccagatcgaa gcggacaaat ccggtaccgt gaaagcaatt ctggtcgaaa
    16081 gtggacaacc ggtagaattt gacgagccgc tggtcgtcat cgagtaacga ggcgaacatg
    16141 ctggataaaa ttgttattgc caaccgcggc gagattgcat tgcgtattct tcgtgcctgt
    16201 aaagaactgg gcatcaagac tgtcgctgtg cactccagcg cggatcgcga tctaaaacac
    16261 gtattactgg cagatgaaac ggtctgtatt ggccctgctc cgtcagtaaa aagttatctg
    16321 aacatcccgg caatcatcag cgccgctgaa atcaccggcg cagtagcaat ccatccgggt
    16381 tacggcttcc tctccgagaa cgccaacttt gccgagcagg ttgaacgctc cggctttatc
    16441 ttcattggcc cgaaagcaga aaccattcgc ctgatgggcg acaaagtatc cgcaatcgcg
    16501 gcgatgaaaa aagcgggcgt cccttgcgta ccgggttctg acggcccgct gggcgacgat
    16561 atggataaaa accgtgccat tgctaaacgc attggttatc cggtgattat caaagcctcc
    16621 ggcggcggtg gtggtcgcgg tatgcgcgta gtgcgcggcg acgctgaact ggcacaatcc
    16681 atctccatga cccgtgcgga agcgaaagct gctttcagca acgatatggt ttacatggag
    16741 aaatacctgg aaaatcctcg ccacgtcgag attcaggtac tggctgacgg tcagggcaac
    16801 gctatctatc tggcggaacg tgactgctcc atgcagcgcc gccaccagaa agtggtcgaa
    16861 gaagcaccag caccgggcat taccccggaa ctacgtcgct acatcggcga acgttgcgct
    16921 aaagcgtgtg ttgatatcgg ctatcgcggt gcaggtactt tcgagttcct gttcgaaaac
    16981 ggcgagttct atttcatcga aatgaacacc cgtattcagg tagaacaccc ggttacagaa
    17041 atgatcaccg gcgttgacct gatcaaagaa cagctgcgta tcgctgccgg tcaaccgctg
    17101 tcgatcaagc aagaagaagt tcacgttcgc ggccatgcgg tggaatgtcg tatcaacgcc
    17161 gaagatccga acaccttcct gccaagccca ggcaaaatca cccgtttcca cgcacctggc
    17221 ggttttggag tgcgttggga gtctcatatc tacgcgggct acaccgtacc gccgtactat
    17281 gactcaatga tcggtaagct gatttgctac ggtgaaaacc gtgacgtggc gattgctcgc
    17341 atgaagaatg cgctgcagga gctgatcatc gacggtatca aaaccaacgt tgatctgcag
    17401 atccgcatca tgaatgacga gaacttccag catggtggca ctaacatcca ctatctggag
    17461 aaaaaactcg gtcttcagga aaaataattt gtgacgcgcg gtgttttcat cgcgcttagc
    17521 aaagaacgta atcaacgccg ggcctgttaa cgctgcccgg cgtttttatt gccgttagct
    17581 aacggtagcc tgcaatttcc gctttcaacg cgctcatttc agcatcgcca tcaacaattc
    17641 ccagctcaaa gcagtatttc tttgtttccc ctggctcaat aaagggcaga cgcccctgcg
    17701 ctcgttcttt atcacgaccg tcaggaaaac tattactgac ctccagtccc ataacatact
    17761 cacccgcgcc taaatttttc cactggataa agttgggtag ctgcatggtg tcgaatttct
    17821 cgtaaattcc ccaccccagt tcggcgttca ccagagcaac tttactcatc ccctgactgt
    17881 cgctattcaa cgtgagatag taaacctgct cgtcaaaacc gggctgtggg gtacaaatct
    17941 ccagatgact ggttaaccct tccgccgcat gcggagttcg tggcgttacg cttttagccg
    18001 acagcaagat ttctggcctg tggcgataat aaaggccagc cgtaattaaa gtgataaaga
    18061 atttgcagcg gctggcgggt gaaaccatga ttagtcacct tgtcttcaat gcgcagcaca
    18121 ttttcgccat aacggcagcg aatggtcctt tccagcgtca gattttcgcc gaacagtcgc
    18181 gtttcccgta ctttgccatt gatgacaaat tcaatgccgt cgtcggtccg ttctgtgcga
    18241 taccccacct cttctgctgg agtcgcagcc aaacgcccgt gtaaccccag cgtttctcct
    18301 tcatcctcac aaggcgtgcc aatgtagcta agtccgcagg tagtcagtaa ccccgcgtaa
    18361 aagctacgta aaaatccgtt gccctgagac tcaaaaaaag cgggggcgac aatgccgttg
    18421 ggggtgataa aagaaagcgc cgtgtccttg tagctggccc atgcaatatc cagcgcgcga
    18481 tcttttacca ccgtaaatgc cagtcccgca ccagtacgga catcaaacgc ttctgtaccc
    18541 tttgctctgc cactacgtaa ctcatagtgt ttaattccgg caacctgcga gagatcgcca
    18601 atccacgacc aaatttcgtt tttcattgtg ctgcttcccg aatggacatg tcatggcgct
    18661 ggatgaacgc ttcgatttcc gcaaggcgag acgttccctc cattgggcca cgtctggtta
    18721 ccgccagcgc accgcaggca ttggcatatt gcagcgctcg atggacgtca aaccctagct
    18781 ggcggcaggc aatccacgcg ccaccaaagc agtctcccgc accggtcggg tcaacttctt
    18841 ccaccggata gctctcaaca tgaaactgct cattcgcgga gtagtaactg gcaccttgat
    18901 tgccgcgctt cacaatcacc tctttaacac cttcttcaag aaaacctgcg attgcacgct
    18961 ccggcgtcga atgtggcgac aagagcaaca cttcgccctc gctgggcata tagatatccg
    19021 ttaactcaag aacaaagtgg agggcgtcac gcatttctgg aatatcgagc atctctttac
    19081 gaatattagg gtcgaaagaa ataacaccac catttgcttt aacgatagtt accgcttttt
    19141 ttactgcgtc taccatatga aacgaaaaca gggaagagcc cataatatga aaatgggtac
    19201 agtccttcag aatattttca tcaacatgct gcgccgataa tttaccgcaa gcggcattct
    19261 taatattaaa aataaaatcg cgatcgccag agttatgata agtaacaaag gcactcccgg
    19321 tagcttcctg aggtaagact gaaatccccc tgatatccac accgtcagca gccagacgat
    19381 gtatattaat atcaccaaag ccatcattcc ccacacagct aatgatgccg cacggaacgc
    19441 caagacgtgt cacctggtcg ataaaaatag cgggcgcacc actgggataa gggccgttcc
    19501 agatacctgg ttgatcaaat ggctggccga ttttactcgc cataatttca accagaattt
    19561 ctccaattgt gaagacctta ctcatatgat taattccttg aaatgattag ctgcgttttc
    19621 ttttatttct tacgtcaaaa taaacggcta caagaatgac taatccttta accaccatct
    19681 ggtaaaaata gggaacattc attaagttca tgccattatt aataacgccg atagttaatg
    19741 cgccgatcac cgtccccacc acggttccat agccgccgtt caggctggtg cctcccagta
    19801 cagccgcagc aatagcatcc agttcgtagc tcaacgcgga attcggttac ccggaataga
    19861 ggcgtgaggc gaggatcatc ccgctgatac ctgccagcac ggctgtaatc ataaagactt
    19921 taattttcaa gcgggtgaca tttatccctg aataaattgc cgcttcttta ttaccaccag
    19981 tgatataaat ttttcgtcca aaggttgttt tagtaagaat gaagtggtta atcagcaata
    20041 agatgattaa tatccagatt ggaataggta tatagagaaa ttctccattc cccagtgcca
    20101 gaaaggcatc atctttaatc atgatgggca taccatcggt cactatataa gcgataccac
    20161 ggaagatccc catagtagca acggtgacaa taaacgacgg gatccccgct atagccgtca
    20221 agccaccatt tatcatcccc agagccagtg ctgcaataat ggataatggg accaataaaa
    20281 taaatggaat tccccatgaa gccccaagcg cagcaaatgt gccaatcaat gcaatggttg
    20341 aaccgaccga taaatcaata tcgccaagca aaagtacata agtcattcca taggcggtaa
    20401 ttgcaattat cgaaacctga agaataatat tagtaatgtt gttggcggta aaaaaactgt
    20461 cattaataaa actgaacaga atgattaaca ccaccattcc actgaggata ccgccatatt
    20521 gcctgaagac ttttttaaaa ttatttgcaa tgggaatttc cgaaggtttc ggactggcaa
    20581 gagttttctc tgtcatttaa cgtactcctg ttgcagcggt cattacctgt gtctgcgtta
    20641 aattttcaca tgaataaata tcggcaatcg ttttattacg cattaccacc agacgatcgc
    20701 taagtgccat gacttctggc agttcagagg acaccagaat aattgctgtc cctgccgcag
    20761 ccagttgacg tatcacgtta tagatttcaa attttgcgcc gacatccact ccgcgcgttg
    20821 gctcatcgag caataaaata cgcggagttt tctccagcca cttcgataac acgattttct
    20881 gttgattacc gccgctcagc gctcccactg cctgatgtac agaactcact ttagtgttca
    20941 ttctggcaat tgattgcagc gtcttcgcct cttcttgtcg gtgattgatg agtcccatcc
    21001 gcgaagcgtt atattgaaac gccacatggt gcgtgttcat attgacgtcg tgcatcagca
    21061 ctaacccttc ttccttgcgg ttttcggtca caaaagcaat gccatgatca atagcctgtt
    21121 taggattagt gatcctgact tccttaccat caatcaacac agtcccctga tacgaaacga
    21181 ggccaaagag cgccttcatc acatcggaac gcccggcccc caccaggcca aaaaatccga
    21241 ggacctcccc cggacgcaca gcgaaactga tctctttaaa aagcgaagga tgggaaagat
    21301 tttttacttc cagttttgca ggaacatttt catccggtat ttccccaaga cgtgctggcc
    21361 aaatattttt catttcccga ccaaccattt gggcaatcaa atgctggata tcactgtttt
    21421 tcttttcatg cgtggcgata tactcaccgt cgcggatcac cgaaatatca tctgaaatcc
    21481 gcataatttc atccatgcga tgagagatat aaataaccgc acagcctcgc tgctttaatt
    21541 tttcaataat gttaaacaga atctccgctt cattatcact tagcgaagat gtcggttcat
    21601 ccataatcac gacatccgct gcatagctta aggctttagc aatttctacc agttgcctat
    21661 gtgccatcga catattgccg accatttcta gtgggtctag tggtatgccc cgttccgcga
    21721 ggagattctg gcaatctgcg agcatttttt tatcgttaat aatgccaaaa cgtcgcggtt
    21781 cattattggc ataaatattt tccgccacgg tcatattatt actcaggctc aattcctgaa
    21841 aaataatcgc aattcctaac tggcgtgaat gctgcggatt attaatggtg atagtttcac
    21901 cacgtagtgt tatcgttccc tgggttggcg gataaacacc ggccagtatt ttcattaatg
    21961 tcgatttacc ggcaccattc tcgcctaata aagtatgcac ccgcccttta cgtaaagaga
    22021 gggtaatatc ttttaatgct tgaacatgac caaatgtctt cgcaatatgc gaaacatcaa
    22081 gaataacatc cgtcataatg acctcggtgt cacaacaaaa cgccaataac gttttgttgt
    22141 gaggtgaaga tgtttactat ttacctgtat aaacgccggg ttgtaccggg atcatttttt
    22201 ctaccggttt accattgagg tgatcaacag cggcatcaat ggctttagca cccatttgat
    22261 cgggatattg ccgaatcacg gccgcaaacg ttttttcact gtcaacggca tcacgcgctt
    22321 ctttcatgcc atcaaaacca atgattttga tgttgtcgtt atgcgcagat tttgctgcga
    22381 taaccgctgc cagcgccgca tcatcaccaa agccaaacag accattcagg ttggcatttg
    22441 cctgcataat gttttgcgca gtcgttaacg cttcagcccg cgtaatacta ggctgttcag
    22501 caacaatttt aatatccggt gtatcgctta accctttctt aaaaccatca acgcgcgcaa
    22561 ccaccgattg tagtgccgga taagaaatac tggctacagt acctttacca ttcaaaagtt
    22621 tgcccattaa cttgcctgct tcaacgcctc ccgcatagtt atcggtcgca acatgcgata
    22681 cgaccggaac gccttccgca gcgacgtcga cggtgataac gggaataccg gccttatcgg
    22741 ctttaattat cgccgcctgg acgcctttcg aatcgaccgg agagataata atggcatcca
    22801 cttttttagt aataaaatcc tcaacatcag aaagttgttt gtttaaatcc tgattggcaa
    22861 atag
//
LOCUS       DADLFA010000053        22776 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000053 DADLFA010000000
VERSION     DADLFA010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22776
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.053"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..454)
                     /locus_tag="JQH65_001720"
     CDS             complement(<1..454)
                     /locus_tag="JQH65_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY6139168.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLET"
     gene            complement(484..1200)
                     /locus_tag="JQH65_001721"
                     /pseudo
     CDS             complement(484..1200)
                     /locus_tag="JQH65_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            1496..2272
                     /gene="exuR"
                     /locus_tag="JQH65_001722"
     CDS             1496..2272
                     /gene="exuR"
                     /locus_tag="JQH65_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HAY6139169.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            2617..3279
                     /locus_tag="JQH65_001723"
     CDS             2617..3279
                     /locus_tag="JQH65_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY6139170.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            3283..3666
                     /gene="mzrA"
                     /locus_tag="JQH65_001724"
     CDS             3283..3666
                     /gene="mzrA"
                     /locus_tag="JQH65_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HAY6139171.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            3813..4181
                     /locus_tag="JQH65_001725"
     CDS             3813..4181
                     /locus_tag="JQH65_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HAY6139172.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            4219..4524
                     /locus_tag="JQH65_001726"
     CDS             4219..4524
                     /locus_tag="JQH65_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HAY6139173.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            4527..4931
                     /locus_tag="JQH65_001727"
     CDS             4527..4931
                     /locus_tag="JQH65_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6139174.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            4921..5220
                     /locus_tag="JQH65_001728"
     CDS             4921..5220
                     /locus_tag="JQH65_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6139175.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            5316..5798
                     /locus_tag="JQH65_001729"
     CDS             5316..5798
                     /locus_tag="JQH65_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HAY6139176.1"
                     /translation="MILSIDSNDANTAPLHKKTISSLSGAVESMMKKLEDVGVLVARI
                     LMPILFITAGWGKITAYSGTQQYMEAMGVPGFMLPLVILLEFGGGLAILFGFLTRTTA
                     LFTAGFTLLTAFLFHSKFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIDRLLNKKW
                     "
     gene            5868..6771
                     /locus_tag="JQH65_001730"
                     /pseudo
     CDS             5868..6771
                     /locus_tag="JQH65_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
     gene            7064..7428
                     /locus_tag="JQH65_001731"
                     /pseudo
     CDS             7064..7428
                     /locus_tag="JQH65_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            7670..8026
                     /locus_tag="JQH65_001732"
     CDS             7670..8026
                     /locus_tag="JQH65_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAY6139177.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGVWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
     gene            complement(8077..8973)
                     /locus_tag="JQH65_001733"
     CDS             complement(8077..8973)
                     /locus_tag="JQH65_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY6139178.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEAQVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            9078..9779
                     /locus_tag="JQH65_001734"
     CDS             9078..9779
                     /locus_tag="JQH65_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HAY6139179.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            9802..9966
                     /locus_tag="JQH65_001735"
     CDS             9802..9966
                     /locus_tag="JQH65_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6139180.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(10100..11410)
                     /locus_tag="JQH65_001736"
     CDS             complement(10100..11410)
                     /locus_tag="JQH65_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HAY6139181.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNS
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(11438..12770)
                     /locus_tag="JQH65_001737"
                     /pseudo
     CDS             complement(11438..12770)
                     /locus_tag="JQH65_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
     gene            complement(13044..14408)
                     /gene="tdcG"
                     /locus_tag="JQH65_001738"
     CDS             complement(13044..14408)
                     /gene="tdcG"
                     /locus_tag="JQH65_001738"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HAY6139182.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGALSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(14480..14869)
                     /locus_tag="JQH65_001739"
     CDS             complement(14480..14869)
                     /locus_tag="JQH65_001739"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HAY6139183.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(14883..17177)
                     /gene="tdcE"
                     /locus_tag="JQH65_001740"
     CDS             complement(14883..17177)
                     /gene="tdcE"
                     /locus_tag="JQH65_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HAY6139184.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFEAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNIMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(17211..18419)
                     /gene="tdcD"
                     /locus_tag="JQH65_001741"
     CDS             complement(17211..18419)
                     /gene="tdcD"
                     /locus_tag="JQH65_001741"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HAY6139185.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(18445..19776)
                     /gene="tdcC"
                     /locus_tag="JQH65_001742"
     CDS             complement(18445..19776)
                     /gene="tdcC"
                     /locus_tag="JQH65_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HAY6139186.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(19798..20787)
                     /gene="tdcB"
                     /locus_tag="JQH65_001743"
     CDS             complement(19798..20787)
                     /gene="tdcB"
                     /locus_tag="JQH65_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HAY6139187.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(20886..21824)
                     /gene="tdcA"
                     /locus_tag="JQH65_001744"
     CDS             complement(20886..21824)
                     /gene="tdcA"
                     /locus_tag="JQH65_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HAY6139188.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRLLIEVG"
     gene            22013..22357
                     /gene="tdcR"
                     /locus_tag="JQH65_001745"
     CDS             22013..22357
                     /gene="tdcR"
                     /locus_tag="JQH65_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HAY6139189.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
BASE COUNT         5969 a         5763 c         5308 g         5736 t
ORIGIN      
        1 ctgtctccag aacacctttg gtggtttgag caaacatcag gtttttagcc agagaaccgt
       61 tagttaacgc atcggaagca aagattttgg ttgcggtaaa agtggttgcc gcatcagctt
      121 taatttcttc accgccccag gttactgtca gcggaacaat cttgctagca tccaggcttt
      181 gaaccagttg cgccggggaa actaactgca tttttacatc tttggtaacg tcgttagacc
      241 agattttggt catcaactgg taagattgca gaccctgccc cggcagatat tgcatttcta
      301 ctgctttcgg caacgcggtg ttatcggtct gtgtcatatc cagagcggca tcaacgttgg
      361 ccgttacagt aatatccttt tgtactgcca atgcggagcc tgcaacagaa aacatcgctg
      421 cgaccagaag agattttgca aatacctttt tcattactat tccttcgttt attacaatga
      481 taattaatgg accgccagtt caaactcgcc attatctttg gtgatccagt tattatattt
      541 gacaacgatt ttgtcgtagc cagatatgct ttcaagattt ctttcctgac cagggaagat
      601 gtttttattt acggtctgta tcttacaatt ttctttgcta ttatttttac aataagcgat
      661 cgttaaaatc tttaagcgtt gattccctgc attaactaat ttttgggcag catttacttc
      721 taacttagca ataggttgct gaggtggaac actaacaaca atcccccaaa ttaagctaac
      781 agttaactgc atagagaaat cttttttatc tgtggcaacg tcatcaatac tgggaaccgc
      841 ttcaaattta acgcgttaat ttttttcttt ttgctctggt tccattgcaa caaagcgcac
      901 agttttgcta ctaccggctg gtaaagcgaa tttaggtggc ataacaacca gatgatttgc
      961 atcaccggat ttaatctcaa cttcattttc ctgaggcgtc gatggattat cgatacggaa
     1021 tactgtcgcc ttgatgtact gaacttcatt acttttagat attacgcgaa cgttgccttc
     1081 atcctgacta ttaatggaaa cagccatagg ataaacagtc atatttgact gtgccaaaaa
     1141 aggagcgaac acgaataaaa gcggcgcaag ccgtgataag agattaattt tcgccaccat
     1201 ataaatttga tcttacaata ttgtttccaa aaagttggtg tattagagat gtagatttat
     1261 tgtaagtcaa tataaaaaag catttttagt taatattaaa tttaaaggct gggaattaac
     1321 ttgctgaaat ttagtagctt tcacaatgac gatacttatt ttttaggaat ttataaatat
     1381 tcacaatatt aattcaatac attaattcaa tcaccaaatt tatatatgac caatggcata
     1441 cccatgaata aagtggtatg ataacttcgt ccgcattcca ctccgggaga cgcatatgga
     1501 aatcactgaa ccacgccgtt tgtatcaaca acttgccgct gacctgaaag agcgcattga
     1561 acagggcgtc tatctggtgg gtgataaact gcctgccgaa cgctttattg ccgatgaaaa
     1621 gaacgtcagc cgaacggtag ttcgtgaagc catcatcatg ctggaagttg aaggctatgt
     1681 ggaggtgcgt aaaggttcag gtattcatgt ggtttccaac cagccgcgcc atcagcaggc
     1741 ggctgacaat aatatggaat tcgccaatta cggtccgttt gagttgcttc aggctcgcca
     1801 gctcatcgaa agtaatattg ccgagttcgc ggcaactcag gtaacgaaac aagacatcat
     1861 gaaactgatg gccattcagg aacaggcgcg cggcgaacaa tgcttccgtg attccgagtg
     1921 ggatttacag ttccacattc aggtagccct ggcgacgcag aactccgccc tggcggccat
     1981 cgttgaaaaa atgtggaccc agcgtagcca taacccgtac tggaaaaaac tacacgaaca
     2041 cattgattcc cgtaccgttg ataactggtg tgatgaccac gatcaaattc tcaaggcgtt
     2101 aattcgcaaa gatccccatg ctgctaagct ggcaatgtgg cagcatctgg aaaacaccaa
     2161 aatcatgtta tttaacgaaa ccagcgacga tttcgagttc aatgccgacc gctatctatt
     2221 cgccgaaaac ccggtagtgc atctcgatac ggccaccagt ggcagtaaat gaaactcctg
     2281 tccgacaggc gctgcgacgc gcctgttcgc ttcgtcggta agcgaaaagt ataaagtgtc
     2341 agcctgtgta aatcctctcg ccaccctccc ctgcattcag caaaatcaga ctccacggac
     2401 atgtaatttt gataacgaac aacgttgacc tttgttacaa ttagattcaa tttgaattta
     2461 tgtttttgaa tgctttctta tctcacgatt taacagggaa tagttcaggc tgtgttgatg
     2521 tatcaaaccc gcagaacata ccaaaacagc aataacattg cggtagtgca tcttttaaaa
     2581 ccagcgtggc gttaaccgat tcaccaggaa taatgaatgg aacttttgac ccaattgctg
     2641 caagccctgt gggcgcagga ttttgaaacc ctggccaatc catcgatgat tggcatgttg
     2701 tattttgtct tgtttgtaat tttgttcctt gaaaacggct tgcttccagc ggccttttta
     2761 ccgggcgaca gtttactggt attggtcggc gtgttgattg cgaaaggcgc gatgggttac
     2821 ccgcaaacga ttctgctgct gaccgttgcc gccagcctcg gctgctgggt cagctatatt
     2881 caggggcgat ggctgggcaa tacccgcacc gtacaaaact ggctatctca tttacccgcg
     2941 cattatcatc aacgcgcaca ccatcttttt cataaacacg gtttatcggc gctgttaatt
     3001 ggtcgcttta ttgcgtttgt cagaacactg ctgccgacga ttgccgggtt atcagggctg
     3061 aataacgcgc gctttcagtt tttcaactgg atgagcggtc tgctgtgggt attgatcctg
     3121 acaactctgg gttacatgct cggcaaaacg ccggtatttt taaagtacga ggaccagctg
     3181 atgtcatgcc tgatgctgct cccggtggtg ctgctggtgt ttggcctggc aggttctctg
     3241 gtcgtgttat ggaaaaagaa atatggaaat cgggggtaag ggatgcaaat acctcgcatg
     3301 tcgcttcgcc agctagcctg gtccggcact gttttacttc tggtcggcac gctgttgctg
     3361 gcctggtccg cggttcgcca gcaagagtct acgctggcga ttcgtgccgt tcatcaaggc
     3421 acaacgatgc cagacggttt ttcgatctgg catcaccttg acgctcatgg cattcctttc
     3481 aaaagtatca cccccaaaaa cgacactctg ttaattacat ttgactccag cgaccagagc
     3541 gccgccgcaa aagcggtcct cgacagaaca ttgccccatg gctacatcat tgcgcagcag
     3601 gacaataaca gtcaggctat gcagtggctg acccggttac gggataactc tcatcgcttc
     3661 ggataacttc ctgttatccg aaacatatca ctcactttgg tgatttcacc gtaactgtct
     3721 atgattaatg aggcggtggt taacactacc gcttcgttgt ctggacaatc gttcctttgt
     3781 aacaggtccg aatcacaatg gaaggttcaa gaatgaaata ccgcatcgct ttagctgttt
     3841 ctctctttgc tcttagtgcc ggtagttatg ccactaccct gtgtcaggaa aaggagcaaa
     3901 atatccttaa ggagatcagc tatgccgaaa aacaccaaaa ccagaatcgt attgacggtc
     3961 tgaataaagc cctgagtgaa gtccgggcca actgttcaga tagccagctg cgtgccgatc
     4021 atcacaagaa aatcgcaaag cagaaagatg aggtggcgga acgccagcaa gatttagccg
     4081 aggcgaagca aaaaggcgat gccgataaga ttgccaaacg cgaacggaaa ctggcagaag
     4141 cgcaggaaga gctgaaaaag ctggaagcgc gcgactacta actcacaata gtcactactt
     4201 actcacctgg agaaaactat gtcgaaagaa cacactacgg aacatctgcg tgctgagttg
     4261 aaatcccttt ccgatacgct ggaagaggtg cttagctcat ctggcgagaa gtcgaaagaa
     4321 gagttgagta agattcgtag caaagcggag caggcactga aacagagccg ttatcgcctg
     4381 ggtgaaaccg gtgatgccat cgccaaacaa acccgtgtcg cggcggcgcg tgctgatgag
     4441 tatgtgcgtg aaaatccgtg gaccggcgtg ggaattggcg ctgcaatcgg tgtagtgctc
     4501 ggcgttctgc tgtcgcgtcg ttaattatgg cggacactca tcacgcgcaa gggcccggta
     4561 aaagcgttct gggcatcggg cagcgaattg tttccatcat ggttgaaatg gtggaaacac
     4621 gtctgcggct ggcggtggtg gagctggaag aggaaaaagc taatctcttt caacttttac
     4681 tgatgctggg cctgacgatg cttttcgctg catttggtct tatgagcctg atggtgctaa
     4741 ttatttgggc ggttgatccg caatatcgcc tgaatgcgat gattgccacc accgtggtgt
     4801 tgctgctact ggcactgatt ggcggtatct ggacgttacg taaatcgcgt aaatccacct
     4861 tgctgcgcca tacacgccat gagctggcaa acgatcggca actgctcgag gaggagtccc
     4921 gtgagcagta aagtcgaacg tgaacgacgt aaggcgcaac tgcttagcca gatccagcaa
     4981 caacggctgg atctttccgc cagtcgtcgt gaatggctgg aggcaacagg cgcttacgat
     5041 cgtcgctgga atatgctgct aagtctgcgc tcctgggcgc tggttggcag tagcgtgatg
     5101 gcgatctgga cgattcgcca tcctaatatg ctggtccgct gggccagacg cggttttggc
     5161 gtatggagcg cctggcgtct ggttaaaacg accctcaagc agcaacagct tcgcggttaa
     5221 cttccctctg gccggagcca ttccggccct atccctcaaa ttttttgaag atttttgaca
     5281 gttttccttg ctaacaatca tcattcacca cgtttatgat tctctccatc gacagcaacg
     5341 acgctaatac cgcgcccttg cacaaaaaaa caatcagcag cctgagtggc gcagtggaga
     5401 gtatgatgaa aaaattagaa gatgttggtg tactggtagc gcgcatttta atgccgattc
     5461 tgtttattac cgcaggctgg ggaaaaatca ccgcttactc tggtacccaa caatatatgg
     5521 aagcaatggg tgtcccgggt tttatgctgc cgctggtgat tctgctcgag ttcggtggtg
     5581 gtctggcaat cctgttcggt ttcctgactc gcaccacagc cctgtttact gcgggcttta
     5641 cgctgctgac ggcattttta tttcacagca agtttgctga aggcgtcaac tcgctgatgt
     5701 tcatgaaaaa cctgacaatt tctggcggat tcctgctgct ggcaattacc ggtccgggcg
     5761 cgtatagcat cgaccgcctg ctgaataaaa agtggtaagc acgcgctata cttaacgata
     5821 aaaaacgagg aggaagctcc tcgtttttgc tattggagga gagaaaaatg gggcaactga
     5881 ttgacggcgt ctggcatgac acctggcccc actggcacag gcggttttat cgcagagaaa
     5941 gatcgttatc atctctatgt ttcactcgcc tgcccgtggg cgcaccgcac gctgatcatg
     6001 cgcaaactca aaggactgga accgtttatt tccgtttccg tagtgaaccc gctgatgctg
     6061 gaaaacggct ggacctttga tgacagtttt ccgggagcaa ccggagatac gctctatcaa
     6121 catgaatttc tgtatcagct ttatctccac gccgatccac actacagcgg acgagttact
     6181 gttcccgtgc tgtgggacaa aaagaaccac accattgtca gcaacgaatc agcagaaatc
     6241 atccgcatgt tcaataccgc gtttgatgcg ctgggcgcaa aagcgggtga ttactaccca
     6301 ccagccctgc aaacgaaaat tgacgaactt aacggctgga tttatgacac cgttaacaac
     6361 ggcgtgtata aagctggttt tgccaccagc cagcaagcct acgacgaggc ggtggcgaaa
     6421 gtgtttgaat cgctggcgcg actggagcag attttaggcc agcatcgtta cctgaccggc
     6481 aaccagctaa ccgaagccga tattcgcctg tggactacgc tggtgcgttt tgatccagtg
     6541 tatgtgaccc acttcaagtg tgataagcac cgcatcagcg attacctgaa tctgtatggc
     6601 ttcctgcgcg atatctacca gatgccgggg atcgccgaaa ctgtcaattt cgatcacatc
     6661 cgtaatcatt acttccgcag ccataagacc atcaacccta cggggattat ttcaattggt
     6721 ccgtggcagg atctcgatga accgcatgga cgagatgttc gcttcggtta aaaaaaggca
     6781 ccttacgagg tgcctctgat aattagctaa gtgcatgttg ttcatgtcgg atgcggcgtg
     6841 aacgccttat caaacctaca aaacgtttat attcaatatg ttaaaatgac cgcgtaggcc
     6901 agataagcgt agcgcatctg gcgattttgc gcttgtcatc agtcttagcg caccttcacg
     6961 gcactttatt aattccttga acgaatattt actgccattt cattcacgtc tattcttaat
     7021 ttgctgcttt aaaaacaagt gattgagcaa aattgaggca aaaatggact ggtatctgaa
     7081 agtactaaaa aattatgtcg gtttccgggg tcgtgcgcgg cgcaaagagt actggatgtt
     7141 tattctggtc aacatcatct ttacgttcgt gctggggctg ctggataaaa tgttaggctg
     7201 gcaacgcgcc ggtggcgaag gcatcctgac gacaatctac ggtattctgg tgtttttacc
     7261 gtggtgggcg gttcagttcc ccgcctgcac gacaccgacc gctcggcgtg gtgggcactg
     7321 ttgttcttaa tcccgtttat cggctggctt attatcatcg tctttaactg ccaggcgggt
     7381 acgccaggcg aaaaccgctt tggaccagat ccgaaactgg aaccttaaaa caaatctgct
     7441 cgccggatgt ctgactcatc cggcttttat catcatgttg atgaatgaat aatttactaa
     7501 tgtttattta aaatatttca atgtcgttat ataaattacc tataaaaaac aaccatgtat
     7561 ttatcttaaa tatcaaaacc tttataacat ccgaaaaaga aagatacgaa tagcatttac
     7621 acaatacccg gaatagactt tcgtcacttt taattaaagg gatgttttta tgcagtggta
     7681 tttagccgta ttaaaaaatt atgttggttt ctctggtcgc gcacgtcgta aagagtactg
     7741 gatgtttact ctgattaacg ccatcgtcgg cgctattatc aatgtcattc aattgatttt
     7801 aggtttagag tttccattcc tttctctcat ttacctggca gcaacaatca ttccggtcat
     7861 tgctctgtgt gttcgtcgtc tgcacgatac tgaccgttca ggtgtgtggg cgcttctcta
     7921 tctggtaccg atcattggct ggttagttct gtttgtattt gcctgtttag aaggcaattc
     7981 tggcagcaat cgttacggaa acgatcctaa gtttggttca aattaatctt agaattgggg
     8041 cgatatttcg ccccttttta ttaacaataa taaatattat tttccgctaa aaagtttggg
     8101 aatttcccgc agacaccagg atttcgcttc ccccatactg tcacgacgcc aggccataat
     8161 aatatcgatc tcgctggtcg attccgggct gacgacacgc aaccgccctt ccgcaatatc
     8221 tttttcgacc atcggatacg gcatcgtcgc cacgccaagc cccgccagta atgcctgacg
     8281 tttatcttca atcgtgctca ccgttaagcg cggctgtttg tccagcagct gtacggtcaa
     8341 caccgggcgc tcacgagcgg tatccgctac cgcaattcca cgatatttca cgcgcgtcac
     8401 ttcagataac ggttccggct cctgatgaat cgggtgatca ggcgcggcaa cgtagacgtt
     8461 cattaaggta tagagcttgc gcgagttgat ctccgacgag gaacgaaaat gcatatccgg
     8521 cgcgataacg atatccgccc gcccctgctc cagtcgttcc cacgcccccg ccagcacttc
     8581 ggtgatgatt gccagttggg tattggcttt tgccgccagt ttgtcgatta acgggaaaaa
     8641 ggcaggtgtc ggtacctgcg cctcggtcac aatggtgaga tgcgtttccc aaccgcgcgc
     8701 gagggcttcc gcatcggtag tcagtttatc tgctgcttcc agcaaaacgc gccctcgctc
     8761 cagcaacatc ctcccgacat tggtgaattt ggtgcgatgg cccgagcggt caaacagcac
     8821 cacatccagc tcttcttcca gtttttgcat ggtgtagcta agtgcggaag gcacgcgtcc
     8881 cagctcatcc gccgccgccg caaaactgcc ccggcgatcg atcgcatcca taacccgtag
     8941 tgcttccagc gttaatgccc tttctttggc catttcgttc tcattcagga aatttgaaca
     9001 taccgggcag aatatctggc taacaatgta gcgtccagcc ccttaccata aaaggaagta
     9061 aagagaggtc aagaattatg attactaccc gaactgccag gcagtgtgga caagcagact
     9121 acggatggtt gcaggcccgg tatacttttt cctttggaca ctacttcgac ccgaaattgt
     9181 taggctatgc ctccctgcgt gtgcttaacc aggaagtgct ggccccaggt gccgcctttc
     9241 agccgcgaac ctatcccaaa gtcgatattt taaatgtgat tctggatgga gaagcagagt
     9301 atcgcgacag cgaaggcaat catgttcagg ccagcgccgg tgaggcgttg ctgctctcta
     9361 cccagccggg tgtcagctac agcgaacaca atctcagcaa agacaaaccg ttaacacgaa
     9421 tgcagctttg gctggacgcc tgcccgcagc gagagaatcc gctgattcaa aaactggcgc
     9481 ttaatatggg caagcagcaa ttaatcgcct cgccagaggg tacgatggga agcctgcaat
     9541 tacgccagca agtgtggctg caccatatcg tgctcgacaa aggcgaaagt gcgaatttcc
     9601 agttgcatgg gccacgcgcg tatttgcaat cgattcacgg gaaatttcat gcgcttacgc
     9661 atcatgaaga gaaagcggcg ctgacctgcg gtgatggggc gtttattcgt gacgaggcta
     9721 acattacgct ggttgccgat tccccactgc gcgctttgct gatagatttg cctgtctagt
     9781 tgtttttaca gggagatgat gatgagtaaa aaatcggcca aaaagcgcca gccagtgaag
     9841 cccgtggtgg cgaaagaacc tgctcgcacc gccaaaaatt ttggctatga agagatgttg
     9901 agcgagctgg aagccatcgt cgcggatgct gaaacgcgtt tagccgagga tgaagctacc
     9961 gcgtaaagac aggttgccgg atgcggcgaa aacgccttat ccggcctaca aaatccaaca
    10021 acattaacga attaaacaac ccgtaggccg gataaggtgc gccagcatcg catccggcac
    10081 aatactcaaa acctggcgtt tatctggcct tgctcgccat aatctcgata atctgccgat
    10141 ccgtttgctg catcgaatgg cttgctaacg cacacaggtt ggcaatcgac tgctcaacat
    10201 catgcgccac gatcccttca ttgccggtca cggcggtatc atccagcgcc attaacaccg
    10261 ctttccacgc agccgaagca ctggtcgaaa ccttcatcgc gcagctgttc gacgcaccat
    10321 cgcaaatcat gccgctgaca tcgccgatca tactgctgat cgccatcgag atagtttcat
    10381 aacgcccatc caccagccat gccatcccgg cggcggcccc cattgctgcg gtcgttgcgg
    10441 cacaaagtgc agacaaacgc ggtaactggt tatggatgta aattgcgctc aaatgagaaa
    10501 gcatcagcgc acgcgccagt cgttcatcat ccgctccgaa gtgttctgct acaaccacca
    10561 caggcattgt tgcggtgatc ccctggttac ccgagccgga gttactcata gccggaagcg
    10621 tagcgccgcc catacgcgca tcggatgccg cgctggtacg aatcacaatg gatgaagaga
    10681 gatctttcgc cagcaaaccg cgctcgcact gtttttccag cgtcgcgcca atatgcagcc
    10741 cccacttacc gctcaaacct tcctgcgata acgcacaatt tagcttcgcg gaatcgagaa
    10801 taaagcggat cgccgcaaac gggacttcat tgacgaactt caggatctca gccagcgtcg
    10861 ttctggaaag caccgtcagc ggagactctt gctcgccctc tgccacacac gcctgctggg
    10921 taaacaccac actattgtgc gtctcaatat gcacaatgtt ggtatgcccg ccgacgatgg
    10981 tgacacacgc ccacttctca ccgttccaga ctttggcgcg tgagaagagg atttcattgc
    11041 aaggttcctg gatcttaacg gagactttcc ccgccgccag cagtgctttg gcatcggcaa
    11101 ttgcctgcgc agttgcgtct ttcagcactt ccagcccggc gttggcattt ccacctaacg
    11161 cccccagcgc cgccgcaatc ggcagcccca ccattcccgt gccgggaacg gtgacgccca
    11221 gaccgttctt catcagattt ggcgaaaccc aggcttctac acgttcaacc ggaccttcca
    11281 gttctgccgc agcaaccgcc gccgccagcg ccagtgaaat cggttcagta catcccagcg
    11341 ccggttttac ttcctcctga acggcgagga tgtaacgctg ccataacgga tttaaagtcg
    11401 aatcaaacat attaaaaacc ttaaaatttc aggtaaatca agaaaatgcc aggaacggag
    11461 aaacacaaag caacaaacca gtgacgataa tcaggtacag agacatccct ttgtatttgt
    11521 gcaatgccgg tactttgtaa accagccacg ccgggatcag gcaccctacc atgccgaaaa
    11581 tcgggctaca gatagaggtg aagctcaaca ccggtgcgtt cagtacgatg gcgctccagg
    11641 ccagcaaaat ggcgaaaatc atgatgccgc gctgaacaag attttcgtta atcttctcgg
    11701 caggcatctt gcgacgcagg atgttcatta cgatcccttg cgttgcttcg cgaaagccta
    11761 aatagacgcc aaagaatgca gtcatgacag caaagatatt gagaatgacg ctgaccactt
    11821 tcacccacgc tgcgccgtca ccgctaataa actgtgcggc aatcgccagc gcggaaatat
    11881 tctgctcgta ggcttttacc gcttcgtcat gtcccatcgc cagcgtgaac gacacggcgt
    11941 agaaaaagac ggtgacaaat aaaatgccaa acgcgatatt cattgcccgc aatgctttat
    12001 gccgcgccac ttcaattgat ttttcccgcg agcgataaga gatcaccatc gggcttaacg
    12061 tctggataaa cagaatcgac gtcagggtaa acggcagcgt aataatggcg tttttcacca
    12121 gcagccccag cggcggtagc gaaccgacgt tatacagatg ccacattccc accatcgaca
    12181 cgcccagcgc agcgactacc agcagcttgg tcagcaccat gccggtcgaa attttgaata
    12241 acaatttctc gccgcgtgaa gagatcgcca ccagaatgca aatcagtacc agaccataaa
    12301 agggactgtc tgacagcacc ccttccgtca cgccgaaggt atgcagatag gaagcactat
    12361 cgttggtgat ggcggtggaa tagacgaaca tccaaatcac cagcattacg aaatagagcg
    12421 cacctaacag gatgccccag tttttaccta aataaccgct aatgacactc gggtaatctt
    12481 tacattctgt gtgattctgc cagcgtatta ataaacaacc gctgaaacag atacattgcc
    12541 gggtaaccaa tcaccgatga gagcaaaaat acccacaatc ccatcaaacc gacctgcacc
    12601 gggagaaaaa caatccccgc gccaatcgcc atcccgatac tcataatcac ccagccggtg
    12661 tcagtactgt cgaatttgat cgcttctcgc cactcgctct cactcattcc agcacgaccc
    12721 gccggggtcg aagcgtctgc aatgacgcct ttattcgatg caatttccat aatttctcgc
    12781 tcaatatttt gtagggctta ttattttttt ccgagccgca tcaaggcgat atgcggtcta
    12841 cgtttttgca ggcgagttat tagaataaag aaatgatacg cgcgaggtca gagaaaatct
    12901 tcacaatcaa gactttgaaa taacggagat ggataagaat tttctactaa attaatcgca
    12961 agagaatatt cattctctat ttgcggcaga tcacaaaaaa aggtgcacat ttgtgcaccc
    13021 aaggatgaaa gctgacagca atgtcagccg cagaccactt taatggccag tcctccgcgt
    13081 gatgtttcgc ggtatttatc gttcatatct ttgccggttt catacatcgt ctcgatcact
    13141 ttatcgagtg aaacacgcgg tgccgaggtg cggcgcatcg ccatccgcgc ggcgtttact
    13201 gctttcacgg cattaatggc attacgttca atgcacggga tttgtacctg tccggcaacc
    13261 ggatcgcagg tcagcccaag gttatgctcc atcgcgattt ccgccgcatt gcatacctgc
    13321 gccggactgc cgcccagtag ttcagttaac cctgccgccg ccattgaaca ggccacgcca
    13381 atctccccct gacagccgac ctccgcgcca gagatggagg cgttcatttt atacagcgcg
    13441 ccaatagccc ccgctgccag aaaatagcgg gcaattgacc gctcgtttac cggacgacgg
    13501 aacttatcgt aataagccag cactgccgga ataatgccgc acgcaccgtt agtcggtgcc
    13561 gttaccacgc gcccgccagc tgcgttttct tcactaaccg ccagcgcgta catgttgatc
    13621 cagtcgatga cattcatcgg atcgttagag atgttatcgc tagaaaccag ctgacgacgc
    13681 agcgctacgg cacgacgcgg cacattgagc ggaccaggca gcacgccttc agtgttcatc
    13741 ccgcgttcaa taccgtcatg catcacttgc cagatacggg caaaccctgc gtcaatttcc
    13801 gctttgctgc gcagcgccag ctcgttgtgc atcatcaggc cagatataga caggccgttg
    13861 taatcacaca ttttcagcag ttcacctgct gagtggaaat cgtaaggtac ggacgtttcg
    13921 acatcgtgcg acaggccgaa gtgttcttct tcgacaataa acccgccgcc gacagagtaa
    13981 taggttttac ttaatagcgc ttcctgtccc ttccaggcag tgatccgcat tccgttctca
    14041 tggcgaggca acatttcggg atggaagata atgttctttg ctacaggaaa atcaacaata
    14101 tgcgcaccag atgccactgg cagccgcccg ctgcgcgtta ctaactctat aaatgcaggg
    14161 atctcatcaa tgacaacatc ctgcggactg tttcctgcca gtcccatgat aatggcgaca
    14221 tccgtggcat ggcctttgcc cgtcagtgac aacgccccat acagatcgac cacaatatgg
    14281 ctcgtcgcgg ttaataagcc gctactttcc agccgatcaa taaaactttt tccggcattc
    14341 attggcccca cggtatgcga actggaggga ccaatcccaa ttttgaaaat atcgaatgca
    14401 ctaatcatat ccacaccctc ggattgccgt tcagtgaagt ggagcggaac gaccttgcga
    14461 ccgtcccgct cacgaggctt tacgcactac gtactgcgat agcttcaatt tccagcttca
    14521 catctttcgg caaacgcgca acctgcacac agctccgggt cgggtaggtc gcctgatgct
    14581 catcgaagaa ctgcttatag acttcattga tggtggcaaa atcattcaga tcggtgatga
    14641 acacggtcat cttgatgata tcgcccacgc tcagcccggc agcaaccacg atcgctttga
    14701 cgttttcgag gcttaaacgc gcctgatctt gcacatcagc cgggatctca ccggtctgcg
    14761 ggcaaaccgg aatttgcccg gaggtgaaga ccatgctgcc taaatcaacg ccctgaacat
    14821 aagggccgat tgcgcctggg gcacgttgcg tttcgataat ctttttcata catcctccgg
    14881 cgtcagagcg cctgggtaaa agtacgtgaa ataacatcct gttgctgttc acgggtcagt
    14941 gcgttgaagc gcacggcgta gccagagaca cggattgtca ggttaggata tttttccggg
    15001 tgctcgatgg catccagcag catttcccga ttcattatgt tgacgttgag gtgttgaccg
    15061 ccttcgacat ccgcttcgtg gtggaaatac ccatccagca ggccgacaag gttggtttta
    15121 cgtactggat cttctttgcc cagcgccgca ggaacgattg agaaggtgta cgagatccca
    15181 tctttggcgt aggtgaacgg cagtttcgcc accgacgtca atgaggccac ggcacctttg
    15241 cggtcacgac catgcatcgg gttagcgccc ggcgcgaacg gtgttccggc gcgacgaccg
    15301 tccggcgtat taccggtttt ctggccgtac accacgttag aagtgatagt cagaatcgac
    15361 tgggtaggga cggcgttgcg ataggttggc agcgctttaa ttttcttcat aaagcgttca
    15421 accaggtcgc aggcaatgct gtctacgcgc tcgtcgttgt tgccgtactg cggatattca
    15481 ccgtcgattt caaagtccac cgccaggccg ttttcgtcac ggattggttt cacgcgggca
    15541 tatttgatgg cagacaggga gtccgtcgcc accgacaggc ccgcgatgcc gcatgccata
    15601 gtgcgataga catcacgatc gtgcagcgcc atcagcgaag cttcgtagct gtacttgtcg
    15661 tgcatgtagt gaatgatatt cagcgcgctg atgtactgca ccgccagcca gtccatgaag
    15721 tgatcgaggc tgtccatcac tttgtcgtaa tccagcacgt cgtccatcag cggtgctgtt
    15781 ttcggcccga cctgaatctt cagcttctcg tccaccccgc cgttaattgc gtagagcagc
    15841 gttttcgcca ggttagcgcg tgcaccaaag aactgcattt gcttaccaat caccattggg
    15901 ctgacgcagc aggcaatcgc gtaatcgtcg ctgttgaagt cagtacgcat cagatcgtca
    15961 ttttcatact gcaaggaaga ggtgacgatc gacacctgcg cggcatattt tttgaaggca
    16021 atcggtaatt cttccgacca aagaatggtc aggttaggtt ccggtgccgg ccccatagtg
    16081 tgcagggtgt gcaaatagcg gaaggagttt ttggtcacca gcgtacgacc gtccagcccc
    16141 atcccgccga tcacttccgt cgcccagatt gggtcgccgg agaacagcga atcaaattcc
    16201 ggtgtacgca ggaagcgtac catacggatc ttcatgatga agtgatcgat cagttcctgt
    16261 gcctgctgct cattgagtac gccagcttca aagtcgcgct caatgtagat atcgaggaac
    16321 gatgccgtgc gtcccagcga catcgcgccg ccattttgcg atttcactgc cgccagataa
    16381 gcgaagtaga gccactgcac cgcttcttgc gcattctgcg ccgggcgaga gatatcaaag
    16441 ccatatttcg ccgccatttc ctgaatctgc aacagcgcat gacgatgctc tgccagctcc
    16501 tcacgcagac ggatggtggc ttccagatcc tcgccttttt ccagacgaga ctggagatcg
    16561 gcaaattgca gttcgcgttc acgtaccaga taactgatgc catacagcgc tacgcggcga
    16621 tagtcaccga taatgcgccc acggccatag ccatctggta aaccggtcag cacgccagat
    16681 ttacggcagc gcagcatatc cggtgagtaa acatcaaata cgccctggtt atgggtttta
    16741 cgcagatcgg taaacagata ttcaaattca ctgtccattt cacggccata ggcgtggaat
    16801 gaacttttaa tcatattaat gccaccgaac gggtgtagcg cacgtttcaa cggcgcatcc
    16861 gtttgcaggc caacaatttt ttccagcggc tggttaatat atcccgcatc atgagcggta
    16921 attgtggtgg caatattggt atcgaaatca accggcgcgt gggttgcgtt ttcgatacgg
    16981 ataccttcca ttactttttc ccacaattcc gtggtggcag gcgtcgcttc ggcgaggaaa
    17041 gattcatcgc cttcatacgg tgtatagtta tgttgaataa aatcgcggac attaatttcg
    17101 tttttccagt ccgtaccttt aaagccaagc catgcgtcgg cgtacagctt atcgctggta
    17161 tcaatatcta ccttcatgaa aaataatctc tctacaatac ttcaactaaa ttatgcaaat
    17221 tctgcgggcg cgttaacttt gcctaaatga atggcatcca aagcaatcat tttttcttcg
    17281 ttagtcggaa taacagcgca aatgacacgc gcattttcac tggaaacaat tcgctcacca
    17341 caggagttag agcgattatt catttctgta tcaatctcta cgcctaatac agccaaatgt
    17401 tccatgacca gacgacgaat taagcttgaa ttctctccta ttccgccggt gaatataatt
    17461 ccatccaggc gacgtaatga agctgcgtgt ccggcaatat gacgggcaat tcggtgaaca
    17521 aaggttttaa ttgccagttg cgcgcgttcg tgaccttcat gccaggcttt ttccagaaca
    17581 cgtaaatccg aagaaagacc ggaaatacct aataatcccg actctttatt cactacgcgt
    17641 tccaggtcac ccaagctctg gttggtttgg ctggcgaccc aggacatcgc accaaaatcg
    17701 acatcgccac tgcgggtacc catcatcaag ccttccagcg gcgtcattcc cattgaggtg
    17761 tcaacactct gaccgttgcg aactgcgcag attgacgcgc cattgccaag atgcgccaca
    17821 accaggccgg agtcatcttc cgctagattc agcaaagaat gtgcgcgctg ggaaacatag
    17881 cggtgcgacg tgccgtggaa accataacgg cgtacaccta actcttcata atatttccac
    17941 ggcaggccgt ataaataagc ttccggagcc atcgtctggt ggaaactggt atcaaatacc
    18001 gccacctgag ttacgcccgg aaataattgc tgcgccgatt caataccact taaattggcg
    18061 taattatgca ggggtgccag tggagaaacg cgacggatat tatcaatgac ctcatcggta
    18121 ataatggcgg actcggtaaa aatactgccg ccgtgagcga tgcggtggcc aattaaggcc
    18181 acactgtcat ttaaattccg tttttccagt tcaaatgcaa ttgccttcaa tgcaccttcg
    18241 tagctgtggt gagccagcgg tgctggctct cccccattta cggataagaa tgcattttct
    18301 gagttaatac cgtcggcaat acctgacatt aatacttcac agtcgctggc atcgagtacg
    18361 gaaaacttaa tcgaagacga accacagtta ataaccaaaa caaccggaaa ttcattcatc
    18421 tcttttctca tcctgagtta cggattaaaa cagtttgtat acgatgttca ggatggtcag
    18481 cagaccaatc acggtaacaa acacgttatc cagacgacca cggtatttcg ccagagacgg
    18541 tgctttacgg atggcataca tcggcaacag gcacagcagg gatgcgataa tcggtgcgcc
    18601 catggcttca ataaggtcga ggatgttcgg gttggcgtag gcaacaaccc aggtggagcc
    18661 catgatgaag atcatgctga tagtgttcag tttacccagc gacactttag ttttgtcgcc
    18721 tttataaccg aacttcagaa tcagaccatt caagccttcc agcgtcccca gatagtgacc
    18781 gaagaaagat ttgaagatag ccacgagtgc gatgatggaa gccgcatatt ccagtgtaat
    18841 cgcgaacgtt gttttggtac cggtcatgga cgcaaagtgg ttagccagat aagaaagcac
    18901 tggaatattc tgcgctttgg cttccgccat gttggccgga gacagcgtaa acaggcagct
    18961 aaaggcaaag aacatcacca ctgcaaccat cagcatgctg gcacgagaaa tgatttggga
    19021 acatttacgt tcggtgaagt cgcgaccgaa gtctttctca tactcttcac gtttagaaac
    19081 cacgaaggaa gagacgattg gcgagaagtt aaaggagaaa accatgatgg aaatccccag
    19141 ccagacagtg atcaggatac cgtcatgacc ggttaacgac agcgaaccga ggtcaacctg
    19201 gtcgataact gcagagttcc agtaagggat cagcgacaga gaaatcagca ccaggctggc
    19261 gataaacggc cataccaggt agctcatcac tttaaccatc agatccttac caaaccagat
    19321 gacgaaagcc atcagcagca acaggaacag cgccacaaag ccgcgattca gcggtgcaaa
    19381 tccgagctgg ttttcccaga acgtcataaa ggtattggta atagtaacgc cataaatcca
    19441 cagcagtggg caaatcgcga agaagtacag gaacgtgata accacgccgc cagttttacc
    19501 aaaatgctct tccaccgttt ccgtaatgtt gccggaaggg ttagagccag aaagacacag
    19561 acgcgccagc gcccggtggc aataaaatgc gatggggtat gccaatacca acatcagaag
    19621 aatcgggatc agtccgccaa aacctgcgcg gatagggaag aacagcaccc cggcgccgat
    19681 tgccgtacca aacaagccta acgtccatgt ggtatctgat ttacgccagg acgattgttt
    19741 tgtctggctg gatacaatgc tatctgaagt actcatatcc tatcctcaac gaattaatta
    19801 agcgtcaacg aaaccggtga tttgagagac gcgagaaaga tcgatattgc cgccggaaat
    19861 aatactgacg gtttttctgt tttgaatata ttggtctaat ttaccgctta ataatgcagc
    19921 acatgccaga gcacctgcgc cttcggtgac gactttattg cgctgaatta aggcaatcat
    19981 actgtttctg atttcgtctt cgctgaccag cacgatgtca tcgactaatt cacgaacgat
    20041 ttcgtaagtt aaattacccg ggcgggagac atcacaacca tccgccaggg tgccggtagt
    20101 tcggtgcgtg gttatttctc cggagtggaa agaagccgcc atgccgtgaa cgttttctga
    20161 ctgtacgcca ataacacgaa tggtcgggtt aatagattta attgccaccg caataccagc
    20221 aattaaacca ccaccaccaa ttggcacaat cacgttatcg acatcataga gatcttccat
    20281 aatttccaga ccaatcgttc cctggccagc aatcactttc ggatcatcgt aaggtgggat
    20341 aaaaatacga ccttccattt cgacaatttc gctcacttta gcgatagtgt cgttgaagtt
    20401 atcaccatgc agaacgactt ctgcggagta gtcgcacgtt gccgctactt tggattttgg
    20461 cgcacctttt ggcatcacca ctttaccgtc gatacccagc atcgcgcagg agagcgaaac
    20521 cccttgcgca tggttgcccg cagaacaggc caccacgcct ttgcgttttt ccgcatcggt
    20581 cagtgaactt aatttattaa atgcgccacg aattttaaat gaacccgtac gctgcatatt
    20641 ttcaaacttc aggaatattt cacctttgca acgttcacta aaatagttgg agcgaggcat
    20701 gcctgtttta taaattcgcc cagccagtcg ttgtttcgct tcaataatat catcaatagc
    20761 aaccggcaga tcgtatgtaa tatgcattat aaaacctctt cgcctgaatt aaatatgtag
    20821 ataaccgtaa ccgacacctg caaaacagac aggtggatta tttatgttta aataaaaaca
    20881 aataactaac caacttctat taatagcctt cgtctacacc cattataaga tgaatactct
    20941 ttggctaatt ccaccaaaac cgatgctgct tttttaatac gataattttt cgaccatacc
    21001 gcggcatatt gtgccacagg taatgtttct tcaactggaa tagtaataaa ttgatttgaa
    21061 ccaaaaggtg acgtcatatc acaaggaatt acagttaaga aatcagcatt gagaacaaga
    21121 ttataaattg tcacgactga gtcggtttta acgatgtttt caatactgat gccatttctt
    21181 tgtaacgtag taagaagctc gctgtagtac cccatattgg tttgtggcaa cacccactgt
    21241 tcgttcttca acgactccag cgtggtggtg ccggtgcatg ttcgggactt actggctacc
    21301 agcacaaact cggactcgaa cagcggctca acatgtaaat cctgaagctt catttctgca
    21361 cttaacgtac caatcgcaaa atccagtcga ccgtcgcgga ttgccggtaa gaacgaagac
    21421 agttgcgctt catacataga aacctgcgct ttcgggaaca cctctttgaa cttgttgatc
    21481 atccctgaca taaaagtaaa accaatcaat gaaggaaaac caaatgagac ttccaccacc
    21541 gcctcagaag acataccgct tatctcatta accatatttt tcatttcacg ggtaatggat
    21601 tcggaacggg agagtaacaa ttgaccggca ggtgttaatg ttacaccggt atttttccgc
    21661 accactaatt ccacaccaaa ataatcttca atatcgttaa tgattttact gacggccggt
    21721 tgagttaacc ctaattcttt tgcagccgag ccgatagagc cacttctaat gacttcctga
    21781 aagactacca ggtgctgcgt tttcggaaga agaatagtgc tcataacgac ctacgttaat
    21841 tacctcattg acggcatgaa gtgtatcaaa atgaaatgaa caggatatgt gcgaccactc
    21901 acaaattaac tttcattact ttccagggta tcgttattac aattaaatat ctttaaatat
    21961 caacaagtta aaacataaaa atcagcataa accctgattt ttatcaaaaa aaatgacggg
    22021 gataaccata ttttatggcg ataacatcat tcgttatgtg gttaatacaa aaaaggggct
    22081 gagaccatat ttcaagcaat taccggataa ttaccaggca aaatttgagt taaatttaat
    22141 gagcaagttc tcaaatttta ttataaataa accattttca gcgataaata ctgcggcacg
    22201 tcacattttt tcacgctatt tgttggagaa caaacattta ttttatcaat attttaaaat
    22261 ttcgaataca ggtattgatc atctcgaaca attgattaac gtcaactttt tctcttctga
    22321 caggacgtca ttttgtgaat gcaatcgttt tccataaatt cttctcccct cataggcgac
    22381 gaatagcatt ttgtgttgag gatcacaaaa caaataattg ctggtcgccg cgataaggtc
    22441 agacgaagtc aacaagggga attttcactg tgctttacat cagcgtagga cacagaatgt
    22501 tgcttcgttt actgtaacgc cgggtaaatg agcgtttttt gatagtgcgt aagtgtcgtg
    22561 cgaggaaaat aagcatctat tattgtttgt catgacgaca aggcaggcat caatgaaagg
    22621 cttcccaatt gcacatattt ttcacccttc aatcccgcca atgcacgcag tggttaacaa
    22681 tcacaataga aatattgatt attggacggt aaaaagaaag tttgcagaaa ttgtctccac
    22741 caatgacgtt aaaaaaattt acagtataag taatga
//
LOCUS       DADLFA010000054        22765 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000054 DADLFA010000000
VERSION     DADLFA010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22765
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.054"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            235..718
                     /locus_tag="JQH65_001746"
                     /pseudo
     CDS             235..718
                     /locus_tag="JQH65_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-associated peptidase"
     gene            900..1323
                     /locus_tag="JQH65_001747"
                     /pseudo
     CDS             900..1323
                     /locus_tag="JQH65_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368730.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
     gene            complement(1390..2847)
                     /gene="nuoN"
                     /locus_tag="JQH65_001748"
     CDS             complement(1390..2847)
                     /gene="nuoN"
                     /locus_tag="JQH65_001748"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HAY6139190.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQSLISIVRLAMPLM"
     gene            complement(2854..4383)
                     /gene="nuoM"
                     /locus_tag="JQH65_001749"
     CDS             complement(2854..4383)
                     /gene="nuoM"
                     /locus_tag="JQH65_001749"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HAY6139191.1"
                     /translation="MLLPWLILIPFIGGFLCWQTEHFDVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4547..6388)
                     /gene="nuoL"
                     /locus_tag="JQH65_001750"
     CDS             complement(4547..6388)
                     /gene="nuoL"
                     /locus_tag="JQH65_001750"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HAY6139192.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEILHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYFCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQSVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWQGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6385..6687)
                     /gene="nuoK"
                     /locus_tag="JQH65_001751"
     CDS             complement(6385..6687)
                     /gene="nuoK"
                     /locus_tag="JQH65_001751"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HAY6139193.1"
                     /translation="MIPLQHGLILSAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6684..7238)
                     /gene="nuoJ"
                     /locus_tag="JQH65_001752"
     CDS             complement(6684..7238)
                     /gene="nuoJ"
                     /locus_tag="JQH65_001752"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HAY6139194.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7250..7792)
                     /gene="nuoI"
                     /locus_tag="JQH65_001753"
     CDS             complement(7250..7792)
                     /gene="nuoI"
                     /locus_tag="JQH65_001753"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HAY6139195.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7807..8784)
                     /gene="nuoH"
                     /locus_tag="JQH65_001754"
     CDS             complement(7807..8784)
                     /gene="nuoH"
                     /locus_tag="JQH65_001754"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HAY6139196.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8781..11507)
                     /gene="nuoG"
                     /locus_tag="JQH65_001755"
     CDS             complement(8781..11507)
                     /gene="nuoG"
                     /locus_tag="JQH65_001755"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HAY6139197.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSLE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTQLETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11560..12897)
                     /gene="nuoF"
                     /locus_tag="JQH65_001756"
     CDS             complement(11560..12897)
                     /gene="nuoF"
                     /locus_tag="JQH65_001756"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HAY6139198.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12894..13394)
                     /gene="nuoE"
                     /locus_tag="JQH65_001757"
     CDS             complement(12894..13394)
                     /gene="nuoE"
                     /locus_tag="JQH65_001757"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HAY6139199.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13397..15199)
                     /gene="nuoC"
                     /locus_tag="JQH65_001758"
     CDS             complement(13397..15199)
                     /gene="nuoC"
                     /locus_tag="JQH65_001758"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HAY6139200.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15293..15955)
                     /locus_tag="JQH65_001759"
     CDS             complement(15293..15955)
                     /locus_tag="JQH65_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017490251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HAY6139201.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15971..16414)
                     /gene="nuoA"
                     /locus_tag="JQH65_001760"
     CDS             complement(15971..16414)
                     /gene="nuoA"
                     /locus_tag="JQH65_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HAY6139202.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17045..17983)
                     /gene="lrhA"
                     /locus_tag="JQH65_001761"
     CDS             complement(17045..17983)
                     /gene="lrhA"
                     /locus_tag="JQH65_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HAY6139203.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            18902..20119
                     /locus_tag="JQH65_001762"
     CDS             18902..20119
                     /locus_tag="JQH65_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAY6139204.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20203..20801
                     /gene="yfbR"
                     /locus_tag="JQH65_001763"
                     /pseudo
     CDS             20203..20801
                     /gene="yfbR"
                     /locus_tag="JQH65_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
     gene            complement(20860..22692)
                     /locus_tag="JQH65_001764"
     CDS             complement(20860..22692)
                     /locus_tag="JQH65_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HAY6139205.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPVGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
BASE COUNT         5842 a         6274 c         5697 g         4952 t
ORIGIN      
        1 tgtatagatc taacaaccgt aataaaacaa atactcataa ctcattataa ataatgataa
       61 aataacgtct tttattgata atttatatat gggaaaaatt ccttttgtta tattgtttct
      121 gatttcactc cctctgatta tttttaccaa ccctgggtca ctgcattacc accaacgccc
      181 cgacacgtca gtcctgagca attagaaaaa acagttcgtt atcttacaca aactgtgcat
      241 ccacgtaatg ccgacaatat tgataatctg aataggtccg tagagtatat aaaagagttc
      301 tttgtcagta gcggtgccag agttaccgcg cagggcgtcc ccgattggct attacgactc
      361 tgcgcctggt agccaggatt acctttaccc gcaatgtcct ggctttatcc cgatcggggg
      421 gactttattg cctggtcggc agaatacagg atatcaacgc cgttcgtcag gtaaaaacgg
      481 cattgttatc atcccgggat ttatctgttt attctatgaa tgccccatgg tttattcccg
      541 gtattgattt ctctgaccac ctgaattatt ggcaacacga tattcccgcc gtaatgatta
      601 ctgacaccgc tttttatcgt aataaacaat accacttgcc cggtgataca gcagacagat
      661 tgaattatca gaaaatggct cagatggtgt tataaccttg ttacacaaca gtaaataata
      721 aaaatttctc gaggcgggtc cagaaacagc gacatgtaca ttttattttc cctcgtctgt
      781 tttaagatgc gaaacataaa attcaatgga ggaaaaataa atgggaatga ttggttattt
      841 tgcagaaatt gactcagaaa agattaacca atctgcttga gtctactgaa aaacctttga
      901 tggataatat acatgacacg ctttcaggat tacgccgact ggatattgat aaacgctggg
      961 attttttaca ctttgggctg acaggcaccc ctgcttttga tcccgcgaag aatgatccgc
     1021 tgagtcgtgc cgtgcttggt gaacacagcc tcgaagatgg catagatggc ttcctgggat
     1081 tgacctggaa tcaggaatta gccgcgacaa tcgaccgact ggaatcactg gatcgaagta
     1141 agctgcgtaa acagttttcc attaagcgac tatgaaatgg agatttatcc gggagtgacc
     1201 ttttcagaag agttagaggg gcaactcttt gcctcgatca tgcttgatat ggaaaagctg
     1261 attagcgcgt atcgacgtat gctgcgacaa gggaatcatg cgctgacggt tattgttggg
     1321 tgatggtgtc agagcgccaa aaaaaaccgc cgaacatgtc ggcggttttc tgacttacaa
     1381 actaacagat tacatcagcg gcattgctaa ccgcacaata ctaatcagcg attgtggcca
     1441 tacacccagc accagtacca acagtgcaga aatcagcacc acgataccgc ccgcgctgta
     1501 ctgccagttt gacggtgcat cgcgacccgg ttgttccggt gcgtgcaggt acaggctcac
     1561 cgccacgcgc aggtagtagt agaggccgat tgccgaaccg acaaccacgg cacccaccag
     1621 ccaccacaag tgtgcctgga caccgaccgc cagcacgtag aacttaccga taaagcccag
     1681 cgtcatcggg ataccggcca gagacagcat catcaccgtc atcactgccg cgaggatcgg
     1741 acgatgccag aacagaccgc ggtaagagaa cagggaatca gcatccgggc cacgatacgg
     1801 gctggacatc aggctgacca cgccgaacgc gccgaggctg ctgaacagat aaccggccag
     1861 gtaaacccct accgcttcca tcgacatctc gccggtttgc agcgcaatca gcgccaccag
     1921 cagatagccg aggtgagaga tagatgagta accgagcaga cgtttgatgt tggtctggct
     1981 caacgccatc aggttaccga agatgatgga ggcaaaggcg ataatcgcca gtactacgcg
     2041 aatcgcttcg ctgtcaccca ccggtgcata gaggaacaga cgcatcacca caccgaagat
     2101 agcgattttg ctcgccgtcg ccaggaaagt ggaaaccggc gcaggcgcgc cctggtatac
     2161 gtctggcgtc cacaggtgga acggcaccag agagagtttg aagccgaggc caacaatcat
     2221 catgccgaaa cctgccagca acagcggctc gttgagcata ccgtcgccga ggtttttacc
     2281 cagcgcgaca aacgacaggt cgccagactg cgcatacacc agcgccatac caaacagcag
     2341 gaaagaagac gctgcggcag aaaggatggt gtatttgata ctggcttcca gcgaacgttt
     2401 ctggcggaaa gcgtaaccga ccaggccaaa cagcggcaaa gagatcagtt caataccgag
     2461 gaacagagac gccagatggt ttgcattcgc cagcaggatc ccgcccagcg cggcaattaa
     2521 caccaacagg tagaactcat ccttgttgtc gttatagcct tcaagccacg ggtaggcgaa
     2581 agtacaggtg gcgaggctcg ccaacaatac cagcccggtg taaagcatgg cgaaaccatc
     2641 aacgcgcatc agcggcgtaa cgtccatagc gcccgcctgg ccaacaaacc agagcgaaac
     2701 cagcgccgcg ttaagcccaa taaccgagag cgtagcgttg aggaaatgat tgcgtcgcca
     2761 cgcaatggag agcatcacaa ccaccaccgt taagccgacg atcagcaacg gtagcagtgc
     2821 gatcaggttt tgtggagtta ttgtcatggc gatttacggc cttgtagtag taacggaatt
     2881 aacaaaccac tgctggatat tgccaatcgc ggagtgcgag gtatccagaa tcggctgcgg
     2941 atagaagccc agcagtacca gcagcaccac cagcaacagg atcataaaca gctcacgcag
     3001 cgacatccct ggcagttcct ggctggcaat ctggcttttc gctttaccga agtaagcgcg
     3061 atgcaacatc gccagcgaat aaacagatgc aaagaccagc ccaaaggtag agataacggt
     3121 aatcaccggg acaacctgga agctgccgaa cagaatcata aattcgccga cgaagttacc
     3181 ggtgccaggc atcccaagcg ttgccaccgc aaagaacagc gacaatgctg gcagccattt
     3241 catcttgctc cacagaccgc ccatcatgcg catgtcgcgg gtatggatac gttcataaag
     3301 ctgaccacaa agaataaaca gacccgccgc cgacaaaccg tgcgcaatca tctggattac
     3361 cgcgccctgg taggccaact ggctgccggt gtagatagca atcagcacga agcccatgtg
     3421 ggaaaccgag gtgtaggcga tcagacgttt gatatcggtc tgggcgaagg ccatccacgc
     3481 accgtagaag atgccgataa cacccagcca catagcaatt ggcgcgaact ctgccgacgc
     3541 gttcgggaac agcggcaggg agaaacgcag caaaccgtaa gcggcagttt tcagcaagat
     3601 ccccgcgaga tcaacggaac cggcggtcgg agcctgggag tgcgcatccg gcagccagcc
     3661 atgcagcgga accaccggca ttttgactgc gaaggcgatg aagaagccca gcattaacag
     3721 gtattccaca ccactggaca ttggcgtatt cagcagctct tcatagttga aggtccagac
     3781 gccggtcgca ttgtagtgaa caaaaaccag cgccaggatg gcaatcaaca tcaccaggcc
     3841 actcgcctgg gtgtaaatga agaacttggt tgccgccgtg atacgcgttt taccgtcaga
     3901 ggctttatgc ccccacagtg cgatcaggaa gtacatcggc accagcatca tttcccagaa
     3961 gaagaagaac aggaacatgt cgatggcaag gaacacgccg ataacgccgc ccaggatcca
     4021 catcaggttg aggtggaaga agccctgata tttttcgatc tctttccacg aacagagtac
     4081 cgccagcaca ccgagcagac cggtcagcac gaccatcagc agcgacagac cgtcaatggc
     4141 gagatgaata gagataccaa aacgcgggat ccacggcatg tcgaattcag actgccactg
     4201 cggaattccg gcggattgcg tcagtgaata accgccctgc aaccacagtt gcagcgacag
     4261 cgccagcgtc aatcccatgg tgatcagcgc gatccagcgt ggcaccttga cgtcaaagtg
     4321 ttcggtctgc cagcacagga agccgccaat aaagggaatt aatattagcc agggtagtaa
     4381 catggcgatc tttattcctt gtaaaagtcc cgtcaggacc ggattttcaa cgaattctca
     4441 cgacagaaaa ttctcttcgg actgggggta gtgtcggatg cgccgcgaag gcagcttatc
     4501 cgacctacgt tcttatatgc ccaggggcaa tcccacaatc cttaactcaa cgcagtacca
     4561 tcaacagtgc cagcaccacg accgcaccga tgctcatgga tgccacatac cagcgcagat
     4621 aaccgttctc gcttaacagc aggcctttac ctgcaaagcg ggaaagaacc gccggaatgt
     4681 tcatcattga gttcagcgga tcgcgtttca gcaaccaggc aatacccaga aacggcttga
     4741 cgaacacttt gtcgtacagc cagtcaaagc cccaggcgtt gtaccaccag gtgcccagca
     4801 gacggcccgg cgcactgttg gcaatggagg tcaccagagt acgtttaccc tgccacagcc
     4861 aggctgccag cagaatgccg accaccgcga ccacgccaga ggtaatttcc agggtcaaca
     4921 tgctgccgtg cgccagttcc gtcgtttgcg gaagcacgct ctgcagcggc ggtacaatca
     4981 gtgcgccaac gaaggtggaa aggatcagca gcacaatcag cggcaggctg tgagttaccc
     5041 ctttcacggc gtgagcgtga atttgttctt ttccgtggaa gacgatgaaa atcatacgga
     5101 aggtgtagag cgaggtcata aacgcaccga ccagacctgc caccatcaga ttgatatgac
     5161 cattcgccat cgcacccgcg aggatctcat ccttactgaa gaagcccgca gtgaccagcg
     5221 gtagtgccga cagtgctgcg ccgcccacca ggaagcagaa ataaaccagc ggaatagatt
     5281 tacgcagacc gcccatcttg aagatgttct gttcgtgatg gcaggccaga atgacggaac
     5341 cggatgccag gaacagcagc gctttaaaga acgcgtgagt catcaagtgg aaaatcgccg
     5401 catcccatgc ctgcacgcca agcgcgagga acatgtagcc aatctggctc atggtagagt
     5461 aagcgagaac acgtttgatg tcggtctgta ccagcgcggc aaaaccggcc agcagcagcg
     5521 taaccgcccc gacaatgccc accagatgca gaatttccgg cgtcatcagg aacaggccgt
     5581 gggtacgggc gatcaggtag acacccgcgg ttaccatggt tgcggcgtgg atcagcgcgg
     5641 agacaggcgt cgggcccgcc atcgcgtcgg caagccatgt ctgcaacggc aactgcgcag
     5701 atttaccgac cgcaccgccc agcagcatca gcgtcgccca catcagcatg ttattgccgt
     5761 cagcaaagtg cgctggtgcc agttccacca tttcgcggaa gttcagggtg cccagttcgt
     5821 tgtaaagaat gaacagcgcg aaagcgagga acacgtcacc cacacgggtc acgacgaacg
     5881 ctttcattgc cgctgcgcca ttcttcggat cggtgtaata gaacccgatc agcagatagg
     5941 agcacaggcc cacgccttcc cagccgaggt acatcagcag caggttatcg gcgagaacca
     6001 gaactaccat gctggcgatg aacaggttgg tgtaggcgaa gaagcgagag tagccctctt
     6061 caccgcgcat gtaccaggag gcgtacatgt gaataaggaa acccacacca gtgaccaccg
     6121 agagcatggt cagcgacagg ccgtccagca ccaggttaaa accgatgtta aagtcgccta
     6181 ccgacatcca cgtccacagc ggctggctgt atgcctgctc gccgttagcg aagaaatcaa
     6241 cgccgataaa ggcggttacc agcgccgcca ggcccacaga gccaacgccg acgatcgccg
     6301 aaacgttttc agaccagcgc ccacgggaga acgccagcag gacgaagcca atcaatggca
     6361 aaataatggt taaggcaagc atgttcatcc gcgcatctca cttactgaat cgatgttcag
     6421 gttctggcga cgacggtgaa gttgcagcag cagcgcaagg ccgatactcg cttctgccgc
     6481 cgcgaggctg atggcgagaa tgtacatcac ctgaccgtcg gtctgacccc agtagcttcc
     6541 ggcgaccaca aaggccagcg cggaggcgtt aatcatgatt tccagaccaa tcagcataaa
     6601 cagcagattg cgacggataa ccagaccggt taagccaaga acgaataaga ttgccgagag
     6661 gatcagtcca tgttgtaagg ggatcatgcg tgctcctccg tttttctttt cgcgctgtcg
     6721 tctttacgat tgctcagcac ttcacccgca cgctcttcac gaccgacgtg gaaggccaca
     6781 accagacccg cgagcagcag catagaagcc agttccaccg ccagtacgta aggcccgaac
     6841 agcgtaatac ccactgcttt agcactgatt ggcgtaccgt cgataccctg atcattaaca
     6901 ccaaggatgg cgtaaacaat caccaccagc atgatggccg acaaaattgc cggaccaatc
     6961 cacacctgcg gtttcagcca ctggcgttcc tgttcgattt ctgaaccgcc caggttgagc
     7021 atcatcacca cgaacacgaa cagcaccata atggcacccg cgtagacgat aatttccagc
     7081 gcaccggcga agtaagcgcc cagtgagaag aacaccccgg aaatcgccag cagcgaaata
     7141 atcaggtaca gcagtgcgtg taccggattg gtatgggtga tcactcgcaa ggtcgcaagt
     7201 atggctatca ggccacagat ataaaaagcg aactccattg cccctctcct tacggtaaca
     7261 ggctcttgac gtcgataggc ttggcttcgt tctctgcttc gcccttatct ttgccgtcga
     7321 ttgccatacc tgccatccgg tagaagttat attccgggta tttgcccgga ccggagatca
     7381 gcagatcctc tttttcgtaa accagatcct ggcgcttgta ttcccccatt tcgaaatccg
     7441 gggttaactg aatcgccgtg gtcggacagg cttcttcgca cagaccacag aaaatgcagc
     7501 gtgagaagtt gatgcggaaa aattccgggt accagcgacc gtctttggtt tctgcttttt
     7561 gcagcgagat acagccgacc gggcaggcta ccgcgcagag gttacaggct acgcaacgct
     7621 cttcgccgtc cgggtcgcgg gtcagaacga tacgaccacg ataacggggc ggcagataga
     7681 ccggctcttc cgggtacatt cgcgtttcgc gtttggcgaa cgcgtgcagg ccgatcatcc
     7741 agatactacg aacctgggtg ccgaaaccta ctaacaattc ttttaaggtc atggtcttat
     7801 tgccccttat tgcgcctgcc agagaatgac agccgccgtt accagcaagt tgatcagcgt
     7861 cagcggcagg cagattttcc agccgaagga cattacctgg tcataacgcg gacgcggtaa
     7921 cgacgcacga atcaaaatga acatcatcat aaagaacgcg gttttcaacg cgaaccagat
     7981 gaatggcggt aacaacgggc cttgccagcc accgaagaac agcgtcacca tcaatgcaga
     8041 gatggtcaca atcccgatgt actcacccac gaagaacaga ccgaacttca taccggaata
     8101 ttcaatgtgg taaccatccg ccagttcctg ctcggcttcc ggctggtcaa acgggtgacg
     8161 gtgacatacc gccacgcccg cgatggcaaa ggtaataaaa ccaaagaatt gcgggataac
     8221 gttccacaca tgcgcctggc tgttgacgat gtcggtcatg ttgaatgaac cggcctgcgc
     8281 caccacgccc atcagggaaa gcccgaggaa cacttcgtag ctcagggtct gcgcagaagc
     8341 acgcatcgca cccagcaacg agtatttgtt gttacttgac cagcccgcaa acagcaccgc
     8401 gtaaaccgcc agacctgcca tcatcaggaa gaacaaaatc ccgatgttca ggtcggcaac
     8461 cacccaaccc ggactgactg gcacaatcgc aaaggccagc agcagcgagg taaaggcaat
     8521 catcggtgcc agggtaaaga tgacgcgatc cgagaatttc gggatccagt cttctttaaa
     8581 gaacattttg atcatgtccg caaccagctg gagcgaaccg ccccagccaa cacggttagg
     8641 tccataacgg ttctggaaca gacccagcag gcgacgttcg ccaaagctca tgaatgcccc
     8701 acaggtgaca accaccagca ggatcaccac cgctttgagg atggtcagca ggatctcaat
     8761 cagttccggt gatatccaac tcattgttgt gcctccttga gatcctcaag atgcgcgcca
     8821 gccagcaccg gagcaatgcc ggacataccc atcggcaagc ccacctgccc tgccgtcagt
     8881 ccttcggcga tttcaaccgg cagcgtgacc gtgttgccat cgtaactaaa ggagacgcgt
     8941 gtacctgcgt tcacacccaa cttcgcggca tccgctgggt tgagtttgat gtacggctgc
     9001 ggcatacggc tctggaagac cggagcacgc tgtgacaatt catcgctgcc aaacaggtgg
     9061 taatacggcg caatacgcca tttcccgtcc tgcggctgga agcgtgccgg tacgctggtg
     9121 aagtaatcca gaccattctc gctggtttca aacagacgca cgcccggatc gccaaagcgc
     9181 agtttgccgc ccacttcgtc ctggaatttg ttccacgcct gcggggagtt ccagcccggt
     9241 gcccaggcaa acggcacttg cgaacggtgc gcggtcggct ggttgttacc ttccatcgag
     9301 aaggtgaaca tggtgtcaat atcctgcggc tgacgcggct catgaacgct gatattggcg
     9361 cgcatggcgg tacgaccgct gtaacggtgc ggttcacggg ccagtttctg cccacgaata
     9421 cggaatgtcg catccggcgc agcatctttg atacctgcca gttccgggat tttcgccaca
     9481 acagcgtcaa tcacatggtc gagctgcgtc cagtccactt cacggctcag cagggtgctg
     9541 tgcagcgagt gcagccagcg ccagctttcc agcatgacag ttttgctgtc gtaataagcc
     9601 ggatcgtaaa cctggaagaa acgttgggcg cgaccttcat tattaattac cgtaccgtcg
     9661 ctttcagcaa agctggcggc ggaaagtacc agatgggcgt tttccatgat cgctgtacgc
     9721 tgatggtcaa ccaccatcac cagcggtgct ttagccagcg cagcattcac gcgggtagca
     9781 gaagcgtgac gatgcagatc gttttccaac accaccaccg cgtcggcgcg tccggtttcc
     9841 agttgggtta acgcttcttc aagcgaaccg ccacccataa tgcccagccc catgctgttg
     9901 acggaacggg caatcatggt gataccgacg tcagcgccgc gacctttcag ggctttcgcg
     9961 acgttagccg ccgcctgaat cacctctaag ctaccggcgt tcgtcccgga gataatcaac
    10021 ggtttcttcg cacctgccag tgcctgcacg atgacgtcga ttttgctttg cagctcaggt
    10081 tcgataccgt caaccgctgg tgcagagtta tccagcgcat gggcgatggc aaaacctaaa
    10141 cgcgcctgat cttcaaccgg tgcgcggtaa gtccacgccg cgatatcatc caaacgggtg
    10201 tcatcaacgt tggtaacaaa cagcggatgc ttcgcacgtt gaccgatgtt aaggattgcc
    10261 gcaatctgcc agtcagccac tttctgtgct gccgccattt cgcgcgcttt acctttcacc
    10321 gcctgacgca ctgccagcgc gacgcgcgcg ccggtctggg taacgtcttc gcccagcacc
    10381 agtaccgcat cgtaagattc gatttcgcgc agagccggag tataaatgcc gccttcacgc
    10441 agcactttca gcgccagttg cagacgttcc tgctcaccgt gagcgatacc ggtgtagaag
    10501 ttttcttcgc ccaccagttc acgcagtgca aagttgcttt ccacgctggc acgcggagaa
    10561 ccaataccga tcactttctt cgactgacgc agaatatctg ccgcgccctg cattgcctgt
    10621 tcggcgttga gggtaatgaa atcatcgcca cgacgctgta ctggctgacg cggacgatcc
    10681 ttcaggttga cgtaaccgta accgaaacga ccacggtcgc agaggaagta gtggtttacc
    10741 gtaccgttgt aacggttttc gatacgacgc agttcgccgt aacgttcacc ggggctgatg
    10801 ttacagccga tggaacattg ctggcagatg ctcggcgcaa actgcatatc ccatttacgg
    10861 ttgtaacgct cggagtgcgt tttgtcggta aatacgccgg tcgggcaaat ttcgaccagg
    10921 ttaccggaaa attcgctttc cagcgtgccg tcttccgggc gaccgaagta gacgttgtcg
    10981 tgcgcgccat aaacgcccag atctgtaccg tcagcgtaat ctttgtagta acgcacacag
    11041 cggtagcagg cgatgcagcg gttcatttcg tgagatatga atggccccaa atcctgatta
    11101 cggtgggtac gtttggtgaa acggtagcga cggaagctgt gtccggtcat cacggtcata
    11161 tcctgaagat ggcagttacc gccttcttca cataccggac agtcgtgcgg gtggttggtc
    11221 attaaccact cgaccacact ttcacggaac tgtttcgctt cttcgtcgtc aatggaaata
    11281 aaggtgccat cggaagccgg tgtcatacag gacatcacca ggcgaccacg cgtgtcttcc
    11341 gcgttttggt attgcttcac cgcacactgg cggcaagcac cgacgcttcc cagcgccgga
    11401 tgccagcaaa agtaaggaat atcaaggccc agagacagac aagcttccag caggttgtcc
    11461 gctccgttga cctcgtattc tttgccgtct acatgaattg tagccattag catgcttcca
    11521 gttttctcgg tcagggaccg agcgttaatc gaaattcggt taccagcgct ctttcagcag
    11581 gttcggctga atcccattaa tcaaatgggt attgctgaac ggctgtttga ttcccgcctc
    11641 aaattcttcg cggaaatatt tgatggcgct ctgtaacggc tccactgcac caggtgcgtg
    11701 ggcacagaaa gttttacccg ggcctaagaa tcgacacagt tgctcaagtg tttcaatatc
    11761 gcccggctgg ccttcgccac gctccagcgc acgcagaatt ttcacgctcc acggcagacc
    11821 gtcgcggcac ggcgtacacc agccgcagga ctcacgggcg aaaaactctt ccaggttacg
    11881 caccagcgac accatgttga tctcatggtc aaccgccatc gccagcgccg tacccagacg
    11941 gctgcccgct ttaccgatac tttcgaattc catcggcaga tcaaggtgcg cttcggtcag
    12001 gaagtcagtc cccgcgccgc ctggctgcca ggctttaaat ttcagaccat cacgcatacc
    12061 accggcgtaa tcttcgagga tctcgcgtgc ggtggtgccg aacggcagtt cccacagtcc
    12121 cggatttttc acccgaccgg agaagcccat cagcttggtg ccagcatctt tacttttcga
    12181 gatgttctga taccactcca cgccgttagc gaggatcgcc ggaacgttac acagggtttc
    12241 gacgttgttg acacaggtcg gtttgcccca tacgccggag gttgccggga aggggggttt
    12301 cgagcgtggg ttagcacgac gcccttctag ggagttgatt aacgctgttt cttccccgca
    12361 gatgtagcgc cctgccccgg tatggacgaa cagttcgaag tcgaaacctg ttcccataat
    12421 gtttttgcca agcaagcccg cttcagtggc ttcggcaatg gcgcggcgca gatttactgc
    12481 cgcttcgata tattcgccac gcaggaagat gtagccacgg taagctttca gcgcaaacgc
    12541 ggagatgagc ataccttcca ccagcaggtg cggcagttgc tccatcaaca ggcggtcttt
    12601 ataggtgccc ggctccattt catcggcgtt acacagcagg taacggatgt tcatggattc
    12661 gtctttcggc atcaggctcc acttcaggcc agtagaaaag cccgctccgc cgcgcccttt
    12721 cagaccagcg tcttttacct gattaacgat ttcgtccgga gacagcccgg tcagcgcctt
    12781 acgcgcccct tcgtaaccgt ttttgctgcg gtattcgtcc agccacactg gctgtttgtc
    12841 atcgcgcaga cgccaggtca gcggatgcgt ttcgggagta cggataatgt ttttcattta
    12901 taccgctcca gcagttcagg gatcgcttcc ggggtcagat gcgcgtgagt gtcctcatcg
    12961 atcatcatgt ttggcccttt atcacagttc cccaggcagc aagttggcag cagcgtaaag
    13021 cggccatcaa atgtcgtttg ccctggtttg atgttcagct ttttctcgag cgccgcctga
    13081 ataccctgat acccgttgat atgacagacc acgctgtcgc aataacggat cacatggcga
    13141 ccaaccggct ggcggaagat ctgactgtag aacgttgcca caccttcgac gtcgcttgcc
    13201 ggaataccca gcacatcggc gatcgcgtgg atcgcaccat ccggcaccca gccacgctgc
    13261 ttctgaacga ttttcagcgc ttcaatggac gccgcacgcg ggtcttcgta gtggtgcatc
    13321 tcgtgctcaa tcgcttcacg ctctgccgca ctcagctcaa aagcctcggt ttgtggttgt
    13381 tgattctcgt gcataattag cggtccacat ctgacataac aaaatcgata ctgcccagat
    13441 aaacaatcag gtcagacacc aggctgccgc ggatcgccgc cggaatttgc tgcaaatgcg
    13501 cataactcgg cgtgcggata cgagtgcgat aactcatggt gctaccgtcg ctggtcaggt
    13561 agtaactgtt gatccccttg gtcgcctcaa tcatctggaa agattcattg gcaggcatca
    13621 ccggccccca cgacacttgc aggaagtggg tgatcagggt ttcgatatgt tgcagcgtgc
    13681 gctctttcgg cggcggcgtg gtcagcgggt gatccgcttt gaacgggcct tccggcatgt
    13741 tgttgaggca ctgctcaaga atgcgcagac tctggcgcag ctcttccact ttcagcatta
    13801 cgcgggtgta gcagtcagaa acaccaccac caaccgggat ttcaaagtcg aagttttcat
    13861 agccagaata aggacgcgcc ttacgcacgt cgaagtcgat cccggtagca cgcaggcccg
    13921 cgccagtggt gccccactcc agcgcctctt tcgcgccata ggcggcaacg ccctgggaac
    13981 gacctttcag aatggtattt tgcagcgccg ctttctcgta agacgccaga cgtttcggca
    14041 tccagtcgag gaactcacgc agcaggcgat cccagccgcg cggcaggtcg tgcgctacgc
    14101 cgccaatacg gaaccacgcc gggtgcatac ggaaacccgt gatcgcttcc accagatcgt
    14161 aaattttctg acgatcggta aaggcgaaga acacgggcgt cattgcgccg acgtcctgaa
    14221 taaaggtcga gatgtacagc aggtggctgt tgatacggaa cagttcggag agcataacgc
    14281 gaatgacgtt aacgcgatcc ggcacggtga tcccggccag tttctctacc gccagcacgt
    14341 aaggcatttc gttaacgcag ccgccgaggt attcaatacg gtcggtatac ggaatgtagc
    14401 tgtgccagga ctggcgctcg cccattttct ccgcaccacg gtggtggtag ccgatgtctg
    14461 gtacgcagtc gacaatctct tcgccgtcga gttgtaaaac gatacggaaa gccccgtgcg
    14521 ccgacgggtg gttcggaccg aggttgagga acatgaagtc ctcgttttcg gtgccgcgct
    14581 tcatccccca ctcttccggt ttgaaggtca gggcttccat ctccagatcc tgtttggctt
    14641 tggtcagctc aaacggcgag aattcggtag cgcgcgccgg gtaatcttta cgcagcgggt
    14701 gacctttcca ggtttgcggc atcatgatgc ggcgcaggtt cgggtgaccg tcgaaagtaa
    14761 tgccaaacag atcccaggtt tcacgctcat accagttagc gttcgggaac agtttggtga
    14821 aggtcggtac gtgcaggtcg ttttctgcca gcgccacctt cagcatgatg tcgcggttac
    14881 gatcgataga aatcagatgg tagaaaacgg aaaaatccgc ggcaggtaac ccttcgcggt
    14941 gtgtgcgcag acgttcgtcc atgccgtgta agtcaaacag catgacgtaa ggtttcggca
    15001 gtttctttaa gaaatcgcca acttccagta attgttcacg cttgatccac acaacgggaa
    15061 ccccggtgcg agtcgcctga acagtaaagg catccggccc aaaacggttg cgcagttcgc
    15121 caattaccgg atcatcaaga tgatcgcggg tctgccaggc gggttcttgc gcggttaagt
    15181 cggtcatatt gttcaccatt gcaaatggtc cgtggtgact gtcggcgcag taagcttcgc
    15241 gaaatagatt taggaataag cgaagaaaaa tccctttgcc gacaggcgca aattaaatct
    15301 cgtcaggtgt acgcaggttg gttacggcaa tgcgttcacc gcgcttgcgt tcgcgctctg
    15361 attgcatatt ggcgcgataa acgccctgat cgccaaccac ccaggagagc ggacgacgtt
    15421 ctttgccgat agattcctgc aacagcatca gtgcctgcat gtacgcttca ggacgcggcg
    15481 ggcagcccgg gatatacaca tcaaccggga tgaatttatc gacgccctgc acaacggaat
    15541 aaatatcgta cataccacca gagttggcac aggcacccat tgagataacc cattttggtt
    15601 ccagcatctg gtcatacaga cgctgaataa ccggtgccat tttggtaaag caggttcctg
    15661 caaccaccat caggtcagcc tgacgcggcg aagcacgcaa tacttctgcg ccaaaacgcg
    15721 ccacgtcatg caccgcggta aacgaagtca ccatctcaac gtaacagcag gaaagaccga
    15781 agttatacgg ccaaattgag tttttacgac cccagttaac catgtcattg agcttgccca
    15841 taaacacgtt tttgttaact tcttgctcca gagggtcggt tacgatctcc tgcttttgca
    15901 gggggtaacg gtcgttctca ccgttgggat ctatgcgggt gagcgtataa tccatcttaa
    15961 tgcctcgcgg ttagcgttga cgattagcga tactgttcgt ttccgggttc atacgctcgc
    16021 ggcgtgaacg cgcgggcgtc cagtccagcg cgccaatacg caccagataa accagacctg
    16081 ccagtaacac aaaaataaaa attgcagctt ccacaaagcc tacccagccg ctttcgcgga
    16141 tagaggttga ccatgcgaac agatacagcg cttcaacgtc gaagataacg aagaacatgg
    16201 ccaccagata aaacttggcg gacaggcgta agcgggcgga gccgaccgag tcgataccgg
    16261 attcaaacgg cacgtttttc gacctcgcgc gtgcgcgacc gcctaaaaac caaccaccta
    16321 ccagcattag gcaacacagg ccaatggcaa cgataagaaa gatagcgaat gcccagtgat
    16381 gagcgatgac ttcagtggat gttgacatac tcattgctta ctcatcaaaa gtagcgccag
    16441 attcactgct cttcacggca gatggacgcc acatcgattc atggggagga ataaaaaaaa
    16501 ccttacaatc actgtagaaa ttcttttata cagctaattg atgtggtctt ttactccttt
    16561 ctataacctt ttgtcaactt taacaaaagt ttcttcacat tagtttacat aatatcaaca
    16621 ccattagcat ttaatgccct ttcaccccag atccttgacg actccaggat aattagatgt
    16681 tgttgaatcg tgtccgttgt gaagcaatgg aaaaaatacg ggtctatttt gacaggaatt
    16741 tgtgtcgatt cctcccccca aaagagagta ttttcttgat ctgtgacacg cttttgtcat
    16801 tccataacaa aaacgcagca acaaatttac gtatttttta acatcattgt agcaggtgat
    16861 ttttttcagg cgattatttg tgcgttcggg acgtgaatct ctggtgattg aaaaatgaac
    16921 agttttgtac gttctgcact atgcgaggaa ggatttttac taaaaaaaag ccgctggggt
    16981 ttaaaacacc cccagcggct cgttttttac actattgtct caggaattat ctatcgtccg
    17041 tcgattactc gatatccctt tcaatcaaca atgaatcatc cccttccgga gcagacattg
    17101 ggctgtattg ccacggatta tggtagctct ccatcgcctg atagatcacc tgggccagct
    17161 cattattact ggacgggtca tagcacagca aatattcggt atcaggtagc ggcggtaagc
    17221 catctactcc actcagcaca cgcaggtccg ggctcatcat ctcaaccggc cttgccgtca
    17281 cgccaagacc ggctttcact gccgcacgaa cggccggaag cgtcgaggcg acataagcca
    17341 gtcgccatgg aatatctgct ttattaagcg ttgccagcac catatcgcga aacggacttg
    17401 gatcatccag caatacaaga gggatcggct cgcctttttg caatacgtat tccgctgcgc
    17461 agtaccagtg tgttggcgag gtacgcaggt tcaatgcctt aaacgctgaa ggacgatggg
    17521 tggttaccat caaatccact tcctgggatt ccagcatttc tgccatatag gcattacgtt
    17581 taacgcggac atccagcgct aatttcggat aaaccgaact cacgcgattt aacaggaaag
    17641 gtaagatcgt atcggcagat tcatctgaag caccgatagt taacacgccc tgaagattac
    17701 tgaacattaa tgatgagcag gcctcatcat taaaacgcag gattttcctg gcgtaaccaa
    17761 gaagttgaat gccatgttca gttaacagtt tgttgcgacc gtgacgagcg aacagttctt
    17821 tcccaacgag ttgttccaga cgctgcattt gctgacttac ggcggactga gtacgacaca
    17881 cagcggcagc tgcggcagca aaagtgttca gatcggcaac agcaacaaat gttctcagca
    17941 gatcgaggtc gaggttaatt atcggacgat ttgcacttat catatattat cacttactgg
    18001 cggctcatac tgagctggtt aatgctgtgc acacacaaac aagcaattcc atttgtaatg
    18061 tgcctccctg gcagtttcat ccggaacccg gacgaaagta aaaatgcata tgagttgcac
    18121 taaaaaagcg actcacattg ttccgttata atgcctgaag tagatcacag aatatatctt
    18181 cagggatcgc atatctatta agttactcac tcttttctat ttatgacatg cgcgtgtttg
    18241 tataaatgta aatgtgagtc cttgttccac tctcgtgcag catcgctggt catacgcgaa
    18301 cacgtacaaa cagcaatggt gaggcggcat caagagcagg gatccgtcat ttatgcgagc
    18361 attttacccc aaaaaccttt tatttataag ggccattgcg aatcatctga tgcaaagtta
    18421 tgttatgtta ggcaaagtaa tcttctatta ttaataagca catcaaaacc tttttgaata
    18481 ttaaataata attaattagc atcatcctca ttcattaatt ccgcttaaca atagtttcac
    18541 accttgcccc attacgacac ctcggaaatc atcacgctgt taaactctcc gtaatgcgga
    18601 aatatcatca tcgcgtttca ttagtgaatt cttaaatgag gcattttcac acaattatct
    18661 tacagataaa aaaaccagac ttacaattaa gaatcagaac aattcactat ataacattgc
    18721 atgtaaagca tatacacctc attattttgt catcattaag ttattaacag cacaatcgag
    18781 ccttcccctc tggcaaaatc ttattctgca gaccttcaaa acaccgtcct gggggagtac
    18841 attgttctaa gctgacttcc acggcaggga gtggcgataa cagcaaaaaa ggtcaagatt
    18901 catgtccccc attgaaaaat ccagcaaatt agagaatgtc tgttatgaca tccgtggtcc
    18961 ggtgctgaaa gaagcaaaac gcctggaaga agaaggtaac aaggtactga aactgaacat
    19021 cggcaaccca gccccgttcg gttttgacgc gccagatgaa atcctcgttg acgtgatacg
    19081 caacctgcct accgctcaag ggtattgcga ttccaaaggt ctttactccg cgcgtaaagc
    19141 catcatgcag cactaccagg ctcgtggcat gcgtgatgtt accgtggaag atatttacat
    19201 cggcaatggt gtatcggagc ttatcgttca ggcaatgcag gcattgctga acagcgggga
    19261 cgaaatgttg gttcctgcac cagattaccc actatggacc gcggcggttt cgctttccag
    19321 cggtaaagcg gtgcattatc tttgcgatga atcctctgac tggttcccgg acctcgatga
    19381 tattcgcgct aaaattacgc ctcgtacgcg tgggatcgtt attatcaacc caaataaccc
    19441 aaccggcgcg gtatattcca aagagctttt aatggagatt gtggagattg cacgtcagca
    19501 taatctcatt atcttcgccg atgaaattta tgacaaaatt ctttacgacg acgctgagca
    19561 tcactcaatt gcgccgctgg cacctgacct gctgaccatt acctttaacg gactgtcgaa
    19621 aacgtaccgc gttgcaggct tccgtcaggg gtggatggtg ttgaacgggc cgaaaaaaca
    19681 cgccaaaggc tacatcgaag gtctggaaat gctggcttca atgcgcctgt gtgctaacgt
    19741 tcctgcgcaa cacgccattc agaccgcgct gggtggttat cagagcatca gtgaatttat
    19801 tacccctggc ggtcgtcttt atgagcagcg taaccgcgcg tgggaactga tcaacgatat
    19861 tccgggcgtt tcctgcgtga aacctcgtgg tgcgctgtat atgttcccga aaatcgacgc
    19921 caaacgcttt aacattcacg acgatcagaa aatggtgctg gatttcctgt tgcaggaaaa
    19981 agttctgttg gtgcaaggga cggcattcaa ctggccgtgg ccggatcact tccgcattgt
    20041 cacgctaccg cgtgtcgatg atatcgagct gtctttgagc aagttcgcgc gtttcctttc
    20101 tggttatcat cagctgtaat cttaatttca ctgccggaga ttgcatccgg cagcgttatc
    20161 ccgccacaat gacctgatga tgtcatcata cgtaaggtca ctatgaaaca gagccatttt
    20221 tttgcccatc tctcccgcct gaaactcatt aaccgctggc cgctaatgcg caacgtgctg
    20281 acggaaaatg tgtccgaaca cagtttgcag gtagcgatgg tcgcccattc gctggcagct
    20341 atcaaaaatc gaaaatttgg cggtaatgtc aacgccgaac gtatcgcttt actggcgatg
    20401 taccacgatg cctcagaagt gccaccggcg atctccctac tccggtgaaa tacttcaatt
    20461 cgcaaatcgc tcaggagtac aaggctattg aaaaaatcgc tcagcaaaaa ctggtcgata
    20521 tggttccgga agagctgcgg gatatctttg cgccgttaat tgacgagcat gcatatagcg
    20581 atgaagaaaa atcgctggtg aaacaggcag atgcactgtg tgcatatctg aaatgtctgg
    20641 aagaactcgc ggccggaaat aatgaattct tgctggcaaa aacgcgactg gaagcgacgc
    20701 ttgaagcgcg tcgcagccag gagatggact acttcatgga agtatttgtt cccagcttcc
    20761 atctttcgct cgatgagatt agccaggatt caccgctgta agcagccgga gtctgcgtcg
    20821 catcaggcaa taagcgccgg atgcgacatc aggctcttgt caaaacggaa acagcatcgg
    20881 gatcatcacc acacaaaccg ccatcacgat aatggtgaac ggtaccccca acttcacaaa
    20941 gtcactgaag ctgtaatttc ccggacctaa aaccagtgtg ttaacaggtg aagaaaccgg
    21001 tgtcataaag gcggcggatg ctgccatcgc cacgaccatc gcgaatggat aaggcgacac
    21061 ccccatcgtt ttggcagcag ccagcgcaat cggagccatc aacaccgccg tcgcggtatt
    21121 agagataaat agcccaataa ccgccgacaa gacaaacaaa caccccagca tcatatgtgg
    21181 cccgtaacca ccgccaatgt ccatcagccc tttcaccgcc agcgcgacac ctcccgtttt
    21241 ctgtaatgcc acagcaaacg gcatcatccc aacgatcaaa ataatgctcg gccagtgaat
    21301 ggatttatag gcgctttcag catctataca gcggaatttc cccatcagca ggcaggcgat
    21361 gatagcggca acaggattag gaatttcatc tgtcagcatt aacgccacca ttagcaccag
    21421 acagaaaatg gcatggggtg cctggctgtg cgcgggcgat gcttcactca cctcttccgg
    21481 taagttcagc gctacgaagt cgcggccctg tttggccagc ataccgatca gtttccagtt
    21541 acccacaacc aggatgatat cgcccagcag cagaggctca tccgccagcg aaccttccag
    21601 cgccacgcca ttgcgcttta gccccaccac attcagtccg tagcgggtac gaaaaccaat
    21661 ttcgcgcacc gatttaccaa tcagttctga ctcaggaatt aatgaaatct ctgccatgcc
    21721 cacatcaagg gcctggtcag aaaaatactc gccgcgcagt accatcggct ccagcaattg
    21781 ctcactacaa aattgccgga gatcgacatc agccgcagac atatcaataa gcaaaacgtc
    21841 acgcgcgcga aattcagaaa ccccattaac gttcacgata acgcgacgaa aacgccgcca
    21901 gcgttcaaca ccgatgacgt tagcgccata acgctcacgt aatttgagat catccagccg
    21961 ttgaccaatc attggcgatc cggggcgaat agccagacgt cgcgcacgcc cggtcagtcg
    22021 atattcacgg ataagatcgc gaaaggttcg acgcgtccag ccttcgcgct gcggggtctg
    22081 ggtatcccct ttcagcatga aacgcatcac taacatatac aaaataccca gcaccagcac
    22141 gaccaggcca acaggtgtta cgctaaagaa actgaagccg tgatagcctt cacgcagcaa
    22201 ttcactgttg actaccaggt tcggcggcgt cgccaccagc gtcatcatgc cgctaatcag
    22261 cccggcaaaa cttaacggca tcatcagacg tgacggcgaa gtttgcatac gcatggcaac
    22321 gcttaacacc acgggaataa agatagcgac aacgccggtt gaactcataa acgcgccaag
    22381 ccccgcgacg gtcagcatca acaaaaccaa cattttgatt tcactgttgc ccgccacttt
    22441 gaccagccat gttcccatta cggtggcaac accggtacgg accaaaccat cgccaataat
    22501 aaacaaggcg gcaatcagga caacgttagg atcagaaaag ccggaaaata cttcggggac
    22561 cgtcagcgtt ccgcttaatg caaacgcgac aataacaaac aaagcgaccg catccatacg
    22621 cactctgccc gtcgcaaaca agacgatggc aacggccagt aatgaaagaa cccaaatcaa
    22681 ttcaccgttc acaacttatc cttgttaatt gagggggatg acttgattct gccataaaaa
    22741 agccccgacg agacggggct aaatc
//
LOCUS       DADLFA010000055        22467 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000055 DADLFA010000000
VERSION     DADLFA010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22467
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.055"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            53..244
                     /locus_tag="JQH65_001765"
     CDS             53..244
                     /locus_tag="JQH65_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY6139206.1"
                     /translation="MKYGKLGYIVPRPQKIRGRWLIDRRAVFVGPGETGIAPEIHTGD
                     DDALKEILTHVTEATKKQH"
     gene            213..1319
                     /locus_tag="JQH65_001766"
     CDS             213..1319
                     /locus_tag="JQH65_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY6139207.1"
                     /translation="MSPRPRKNSTDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTD
                     KGKAEKIASTANQRIAAAEAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDTRLKN
                     GDIAATTHKEKTRMAAYLVSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLW
                     VDIYKEAQHAGEVPPGWNPPEATRKPIPKVTRARLTMEDWQKIYNATPEKHFIRNAML
                     LAIVTGQRRDDICHMRFSDVWNEHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGC
                     RDRILSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGIIPPAGKTPPTFHEQRSLSE
                     RLYRAQGIDTKTLLGHKVQATTDRYNDTRGQEWV"
     gene            1499..2293
                     /gene="potC"
                     /locus_tag="JQH65_001767"
     CDS             1499..2293
                     /gene="potC"
                     /locus_tag="JQH65_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HAY6139208.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMISPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            2290..3336
                     /gene="potD"
                     /locus_tag="JQH65_001768"
     CDS             2290..3336
                     /gene="potD"
                     /locus_tag="JQH65_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HAY6139209.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETCIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPGMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(3492..4313)
                     /gene="cobB"
                     /locus_tag="JQH65_001769"
     CDS             complement(3492..4313)
                     /gene="cobB"
                     /locus_tag="JQH65_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAY6139210.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     KIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(4329..5240)
                     /gene="nagK"
                     /locus_tag="JQH65_001770"
     CDS             complement(4329..5240)
                     /gene="nagK"
                     /locus_tag="JQH65_001770"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HAY6139211.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCRGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQQLQAPEIIALYNQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(5269..6513)
                     /gene="lolE"
                     /locus_tag="JQH65_001771"
     CDS             complement(5269..6513)
                     /gene="lolE"
                     /locus_tag="JQH65_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HAY6139212.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDKVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEMTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     LIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(6513..7214)
                     /gene="lolD"
                     /locus_tag="JQH65_001772"
     CDS             complement(6513..7214)
                     /gene="lolD"
                     /locus_tag="JQH65_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HAY6139213.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(7207..8406)
                     /gene="lolC"
                     /locus_tag="JQH65_001773"
     CDS             complement(7207..8406)
                     /gene="lolC"
                     /locus_tag="JQH65_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HAY6139214.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSINPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRMMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGNEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEDSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMIFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPMAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            8668..9741
                     /locus_tag="JQH65_001774"
     CDS             8668..9741
                     /locus_tag="JQH65_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HAY6139215.1"
                     /translation="MKQKELWINQIKGLCICPVVIYHSVITFYPHMTTFPHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            9869..13315
                     /gene="mfd"
                     /locus_tag="JQH65_001775"
     CDS             9869..13315
                     /gene="mfd"
                     /locus_tag="JQH65_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAY6139216.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTKHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDVARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            13459..14421
                     /gene="ldtC"
                     /locus_tag="JQH65_001776"
     CDS             13459..14421
                     /gene="ldtC"
                     /locus_tag="JQH65_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HAY6139217.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVDEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     TRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(14504..14761)
                     /gene="bhsA"
                     /locus_tag="JQH65_001777"
     CDS             complement(14504..14761)
                     /gene="bhsA"
                     /locus_tag="JQH65_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HAY6139218.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISAKAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            15002..15634
                     /gene="comR"
                     /locus_tag="JQH65_001778"
     CDS             15002..15634
                     /gene="comR"
                     /locus_tag="JQH65_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HAY6139219.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(15696..16235)
                     /locus_tag="JQH65_001779"
     CDS             complement(15696..16235)
                     /locus_tag="JQH65_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HAY6139220.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(16462..17766)
                     /locus_tag="JQH65_001780"
     CDS             complement(16462..17766)
                     /locus_tag="JQH65_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAY6139221.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHSELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTHDPDIYAIGDCASCTRPEGGFVPPRAQAA
                     HQMATCAMNNILAQINGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(18166..18708)
                     /locus_tag="JQH65_001781"
     CDS             complement(18166..18708)
                     /locus_tag="JQH65_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY6139222.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(18731..19756)
                     /gene="nagZ"
                     /locus_tag="JQH65_001782"
     CDS             complement(18731..19756)
                     /gene="nagZ"
                     /locus_tag="JQH65_001782"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HAY6139223.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPEKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSTLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELCFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKASSTRLNQLHERW
                     QEEKAGH"
     gene            complement(19767..20591)
                     /gene="thiK"
                     /locus_tag="JQH65_001783"
     CDS             complement(19767..20591)
                     /gene="thiK"
                     /locus_tag="JQH65_001783"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HAY6139224.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDTALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(20575..21213)
                     /gene="lpoB"
                     /locus_tag="JQH65_001784"
     CDS             complement(20575..21213)
                     /gene="lpoB"
                     /locus_tag="JQH65_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HAY6139225.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPIVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQ"
     gene            complement(21227..21604)
                     /locus_tag="JQH65_001785"
     CDS             complement(21227..21604)
                     /locus_tag="JQH65_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HAY6139226.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQKPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(21607..21966)
                     /gene="hinT"
                     /locus_tag="JQH65_001786"
     CDS             complement(21607..21966)
                     /gene="hinT"
                     /locus_tag="JQH65_001786"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HAY6139227.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVVAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
BASE COUNT         5601 a         5900 c         5574 g         5392 t
ORIGIN      
        1 cgacgagtgg aaaccaaaac ggttcagtct cccaataaca aacactaccc tggtgaaata
       61 cggaaaacta ggatacatcg ttccaagacc acaaaaaatt cgtgggcgtt ggctgataga
      121 tcgccgagca gtatttgttg ggccaggtga aacgggaatt gcgccggaaa ttcatactgg
      181 cgatgatgat gcactgaagg agattttaac tcatgtcacc gaggccacga aaaaacagca
      241 ctgacgtagc cggtctttac gaaaagtttg atcgcagaac tggcagagtt tactaccagt
      301 ataaaaatcc tgtgactgga aaatttcacg gactcggaac agacaaaggt aaggcagaaa
      361 aaatcgcttc cacagccaat cagcgaatag ctgcagcaga agctgaatat ttcatgcgca
      421 aaattgatga aagtccgtca gcaacaaaac gtcggggtat cagattaaag gcatgggttg
      481 atcgatatct gaaaatacag gacacgcgac tgaaaaatgg agatattgca gctacaactc
      541 acaaagaaaa aactcgaatg gctgcatacc tggtttcccg tctgggaaac cacccattga
      601 aagaactgga agtaagagac tttgcattaa tactggatga gtggctggat aaagacatgg
      661 tcagcacagc gagagtaaat cgtggattat gggttgatat ttataaagaa gcacagcatg
      721 caggggaagt tcctcctgga tggaatcctc cggaggctac ccgtaaaccg atccctaaag
      781 taaccagagc caggctcacc atggaagact ggcaaaaaat ttacaatgca acgcctgaaa
      841 aacactttat ccgtaacgca atgcttcttg cgattgttac tggtcagcgc cgtgatgaca
      901 tttgccacat gcgtttttca gatgtgtgga acgaacactt gcatatcacc cagggaaaaa
      961 ccggaatgcg tctggcgtta ccgcttacac tacgctgtga tgccattggg ataacgttaa
     1021 aagaagttat tgatgggtgc cgagacagaa tattaagtcc atatctaatc catagtcggc
     1081 accagaaaca accgaagccg atgagtaaag acaacctgag cgactacttt gccaaagcac
     1141 gggatctggc tgggataatt ccaccagcag gaaaaactcc gccaacattt catgaacaac
     1201 gctctctatc agaacggctg taccgtgcac agggtatcga tacaaaaaca ttactaggac
     1261 ataaagtcca ggcaaccacc gatcgctata acgatactcg aggtcaggaa tgggtttaag
     1321 ctggttattt gacaaacacc atatggaata tccgtcacca tctcaggaaa aaagtgttgt
     1381 aaaatgcgtg gcctagtttt ggggaaacgt tttggagaga ttttggagaa aggaaaaaat
     1441 tgaataaatt caaaatcctg aaagtgctcc accttatgaa taagaaggtg gaactcgaat
     1501 gatcggtcga ctgcttcgcg gcggttttat gaccgctatc tacgcgtacc tgtatatccc
     1561 aatcattatt ttgattgtga actcctttaa cagctcgcgc tttggcatca actggcaggg
     1621 ttttaccacc aaatggtata gcctgctgat gaacaacgac agcctgttac aggcagcgca
     1681 gcattcacta acaatggcgg tgttttcggc gacgtttgct acgcttatcg gttcactgac
     1741 ggcagttgcg ctgtaccgtt atcgctttcg cggtaagccg ttcgttagcg gaatgctgtt
     1801 tgtggtaatg atatcaccag atatcgtgat ggcaatttcg ctgctggtgc tgtttatgct
     1861 gctgggtatt cagcttggct tctggtcgct gctgttctcg catatcacct tctgcctgcc
     1921 atttgtggtg gtgacggtgt attcgcgcct gaaaggtttt gacgtgcgga tgctggaagc
     1981 ggcgaaagat ctcggtgcca gcgaatttac aattctgcga aaaatcatac tgccactggc
     2041 aatgccagcg gtggcagcgg gctgggtgtt aagctttacc ctgtcgatgg acgatgtggt
     2101 agtttcttcg ttcgtcaccg gaccaagtta tgaaattctg ccgttaaaaa tttattcgat
     2161 ggtcaaagtc ggcgtatcgc cggaagttaa cgcgctggca accatattac tggtgctgtc
     2221 gctggtgatg gttattgcca gtcagcttat tgctcgtgat aaaacgaaag gtaacacagg
     2281 ggacgttaaa tgaaaaaatg gtcacgccac ctgctcgcgg cgggtgctct ggcactgggc
     2341 atgagcgccg ctcacgccga tgacaacaac acgctgtatt tctacaactg gaccgagtac
     2401 gtgccgccag gactgcttga acagttcacc aaagaaacct gtattaaggt tatctattcg
     2461 acttacgagt cgaacgaaac catgtacgcc aagctgaaaa cgtacaaaga cggtgcgtat
     2521 gacctggtgg ttccttcaac ctattacgtc gataaaatgc gtaaagaagg gatgatccag
     2581 aagatcgaca agtcgaagtt aacgaacttc agcaatctcg atccaggcat gctcaacaag
     2641 ccttttgacc cgaataacga ctactccatt ccgtatatct ggggggcgac ggcgattggt
     2701 gttaacggtg atgcggtgga tccgaaatct gtcaccagct gggccgatct gtggaagcca
     2761 gagtacaaag gcagcctgct gttgaccgac gatgcccgtg aagtgttcca gatggcgctg
     2821 cgtaagctgg gctattccgg taacaccacc gatccgaaag agattgaagc tgcatataac
     2881 gagctgaaaa aactgatgcc aaacgtcgca gcgtttaact ccgataaccc ggctaacccg
     2941 tacatggaag gcgaagttaa cctcggcatg atctggaacg gttctgcttt cgttgcacgc
     3001 caggcgggta cgcctattga cgtggtgtgg ccgaaagaag gcggcatatt ctggatggac
     3061 agcctggcga tcccggcaaa tgccaaaaac aaagaaggcg cgctgaaatt gatcaacttc
     3121 ctgctgcgcc cggatgtggc aaaacaggtt gctgaaacta tcggttatcc aacgccaaac
     3181 cttgcggcgc gtaagctgtt aagtccagaa gtggcgaacg ataaaacgct ctacccggat
     3241 gctgaaacca ttaaaaatgg cgaatggcag aatgacgttg gcgcagccag cagcatttat
     3301 gaagagtatt atcagaagct gaaagcagga cgttaattca gcaagcgggc ggtggtatta
     3361 ccgcccgttt tttatttagc aaggatgtgg ctaatgagtc aggacagtag agttttatcc
     3421 gactctttcg tcggtattgt ggtccgctcg taggcctgat aagcgtagcg catcaggcaa
     3481 tgcttcctgc tttataatcc cttcagcaac ttttcaacaa actcaggcac cacctggctt
     3541 gccgggccgt aatatttctc ggcaaattca ttaccgacct gacttggttc gagattcagt
     3601 tccacggtgt gcgcgccatg cagtttcgct tcgtgaacaa acccagccgc cggataaaca
     3661 tgcccggaag taccaatggc aatgaaaata tcagccatcg acaacgccat ataaatttca
     3721 tccatgccga gtggcatttc gccaaaccaa acgacgtgcg ggcgcaaggg tgccgggaac
     3781 tggcaacaat ggcatttatc ttctggcgta acgtctccgg tccagtcgag aacctgacca
     3841 ctttgcgaac aacgaacttt cagcaattcc ccatgcatat gaatgacatt ggtattacct
     3901 gcgcgttcat gcaggttgtc tatattctgc gttaccagca aaaagcgatc gccgagagcg
     3961 tcttgcagtt tagccagcgc aagatgcgcg gcgttaggct gaatttttgg ctgctgcagc
     4021 tgtcgacgac gggcgttata aaacgcttgc accagttcag gatcgcgatc gaaaccttcc
     4081 ggcgttgcca catcttcaac ccgatgttct tcccacaggc catctgcggc gcgaaaggta
     4141 cgaatacctg attccgcaga aattcctgcc cctgtcagta cgagtactct tggtttttcc
     4201 attgcttccg gcaccacttt atctctgaaa aaaatacgct gacgcaaacg ctcgcgcagg
     4261 cggcgtttat ttttacgaaa acgacttaac cgatgacccc gacgcgacag catagcaacc
     4321 tctgttgttt aatcggttag atgtaggaag gccgcaccac gcattcctcc cgcatcaccg
     4381 tggcgcgcgc gttcaatgcg cggaacacga gctacaggta agagatgacg aggcagcctg
     4441 tccgccagtt gcgttgtgat tgccgggaaa ttcgataagc caccaccaat gacgaccagg
     4501 tcagggtcaa caatggtcag gatatttccc agacaaaccg ctaataaatc cagataacgc
     4561 tcaacgtgcg cccttgcctg ctcatcgcct tgattataaa gcgcaataat ttcgggagcc
     4621 tgcaactgtt gatgataata gtgttgatac agccacgcaa aaccgcgacc agacagataa
     4681 ttctcaatgc agccatgctg accacagccg cagcggcgta acgggaaatc caaccccatc
     4741 atggttaacg catcaaccgg cagacgcata tggccaaact cgccggtaat gtagctttta
     4801 ccggtaatcg gtttgccgtt gaaaatcagc ccaccgccaa cgccggtacc aagaatcaac
     4861 cccatcacca gtggatattg cgtaaattca tcatcccagg cttctgaaag ggcaaaacag
     4921 ttggcatcgt tatcaaggcg tacatcgcga tcaagacgtg cgctcaggtc ggcacgcagc
     4981 ggtttaccgc tggcagcggg gacattggcg gcatacagcg taccatcttc tgtttccggc
     5041 attcccggta taccaatgcc gacagaacct ctacagccaa aacgttgatc agcttcggct
     5101 accagctcgc acactgcatc taaaaatgcg tcatagctgt cacgcggtgt cggcacccgc
     5161 ttttcccact gcaactgccg accgctatca aacacgccaa gcgcaatttt tgttccacca
     5221 atatcaaacc cgtaatacat tgccgctcct tgttttaatg tactgccttt actggccgct
     5281 aaggactcgc gcagggtcaa tattactggc gcgccgcgcc ggataccaac ttgccaaaag
     5341 actcagcaac aatgctgtga ccagtacgta gaagacgtcc agccagtgca attccgatgg
     5401 caggaagtca ataaaataga tatcgctgga gaggaactga tgaccgatca gcttttcaat
     5461 ccgctcaata atcggggtaa gttgcagtga aacaactacg ccgataatca gcccacacag
     5521 gctgccgaac agccctgcca gcaatccata ccagacaaag atggcgcgaa ttaaaccatc
     5581 tttcgccccc agcgttctta atactgcgat atcgccactc ttatctttca ccgccatcac
     5641 taaggtggag acgatgttga aacaggccac gccaatcacc agtaccatcg ccagatacat
     5701 aatggcgcgg atcatctgga tatcgcgata catatagccg taagtaccaa tccagctttt
     5761 aatataaaca tagctgttgg tcatttcacc cgcatcgcgt accagcttat tggcgttgaa
     5821 aacatccgtc attttaaggg caatacctga cacgctggaa cccatatcaa gatattgttg
     5881 ggcatccgcc agcgggatca tggcaaaact gtgatcgagt tggccactca actgcaaaat
     5941 acccgcaacg tgcaaacgca cacgttttgg ctgcatcagt ttatgctcag gattcgagtt
     6001 ggggatcatg atcgacaccc aatcgccctg cttcactttc agcgcatccg ccacgccttt
     6061 gccgatgata atttgctgtt cgcccgcttt aaaattgcgc cacgcatccc cctgaacaaa
     6121 cgagggtaat gcgctcagac gctgttcctg ttgtgggtta acgcccttca cctggattgc
     6181 gcgcagattc gctccacttt ccaccagccc ggtgaaattg atatacggcg cagcggcggc
     6241 aatacccggc actttctgca ctttatccag tgcttcctgc cagttagtcc atggctgatt
     6301 taccgcttcg atttcaccat gcggcaccac cgccagaatg cggttattca gttcgcgttc
     6361 aaagccgttc atcgcgctta agccgacgat caataccgcc acaccgaggg caatgccaat
     6421 ggtagaaatg acggagatca gcgacaccat gccgccgcgc cgccgtccgc gactaaaacg
     6481 caggccaatt aataacgata aaggcatcgc cattactccg cccccatcag gctcagttcc
     6541 gccgtcagac gtccatcacg catttctaac tggcggctca tacgtttcgc cagttgcagg
     6601 tcgtgagtaa ccaccaggaa ggcggtgccc tgcaagcgat tcaattcccc aagcaactgg
     6661 aagatgctgt cggcgttacg cgcatcgagg ttaccggtag gctcatccgc cagtaccaga
     6721 cgcggattat tgaccagcgc acgggcaatc gctacacgct ggcgttcgcc gccagaaagt
     6781 tcagatgggc ggtgattcgc acgatgctcc agccccaccg cttttaacat ctccagtgca
     6841 cggctgttga tttcagcggg ctttttcttg ccaatcagca gcggcatagc cacgttttcc
     6901 agggcggtaa aatccggcag caggtggtga aactgataaa taaagcccag tttctggttg
     6961 cgcagttctg cttttgctgc cgacgacagt ttgctcatcg gttgaccgtt aaagatcaca
     7021 tcgccggagg ttggcgtatc cagtccgccc agcaggtgca gcaaggtgct tttaccggaa
     7081 ccagagctac cgacgatcgc catcatttca ccttcgccga cgctgaaact gacattgtgc
     7141 agtacatcgg tttgcacact gccttcctga tagcgtttgc acaggttgtc gcattgcaac
     7201 aggatcttat tcataacgta aagcctcagc gggttgtgtg gcggcagcgc gccatgaagg
     7261 gtaaagcgta gacagcagcg cgatagccat cgccaccagc gcaataacaa tgacctgtaa
     7321 aggttcgata gccatcggca gcgccgcgcc atcaagcagg acgccgatta ttggcatcag
     7381 attatttaac tggctggcaa gcagtgcgcc aagcgccgct ccgaggatcg caccgataat
     7441 cccggcgctg gccccttgca ccataaagat catcatgatt tgtcgcggag ttaagccttg
     7501 cgtttgcagg atcgctactt cgccctgctt ctccatcacc atcagcccca gcgaggtaat
     7561 aatgttaaac gccgcaacgg cgacaatcag gctcagcagt aaacccatca tatttttttc
     7621 catgcgtacg gcctggaaca actcgccttt acgatcacgc cagtcctgcc atttgctgtc
     7681 ttcaggcagt ttttgctgac ttaatgagtc gactttcagc ggctcatcca gccacaaacg
     7741 ccagccggta atattgcctg ccggataacg catcagacgc gaggcatcct caatattcac
     7801 cagcatttca ttgccatcga cttcactgtt ggcggcgaaa gtgccaatca cattgaacag
     7861 gcgctgactt ggaatacgcc ccatcggcgt gaactggctg gcagatggta ccatcatgcg
     7921 gatttgatca ccgcgattaa cgcctagctg tgaggcaagt tgttcgccga ggatgacatt
     7981 atatttcccc ggctcgaggt cagtttgttt cacattgacc agatacggcg ttagtggatc
     8041 tttttgcgcc ggatcgatac ccagcatcac cccgaccgcc acgctgcgcg cgctttgcag
     8101 taccacatca ccggtagtaa taggtgcgac gcgattaacg ccgtccagtt tgactgccgt
     8161 ttccgggagt tgctgcgggt taatagagcc atgctcagaa gagagaattg cctgtggcat
     8221 caggccaagg atgttgtttt gcagctcgcg ctcaaagccg ttcatcactg acaatactgt
     8281 gaccagcgcc atcaccccga gggtaatgcc gatggtagaa agccaggaga cgaaacgacc
     8341 gaagcgatcc gctgcacgcc cacgcatgta acgcaggcca ataaatagag cgacaggttg
     8401 gtacatgaaa tccgtctggt tgctgtagca aagacacgag tatataagcg aaagcaagat
     8461 gggtaaacgt ctgaactgta agtgatcgtc agggctaatc tgaaaagccc ccgtaaatca
     8521 atcggctaaa ttgccatgtt ttatcatcaa tcttgccgcc tgatcttaat ctgaaaaatc
     8581 atccaataca gacagatacc cattacacct cgggtcataa cccgcaggat caaccgcatg
     8641 taaattaaaa aagagcgtgt tgtcctgatg aaacaaaaag agctatggat taaccagatc
     8701 aaagggttat gtatttgtcc ggtggtgatt tatcactcgg tcattacctt ttatccgcat
     8761 atgaccactt ttccgcatcc gttatcggaa gtcctgagca aatgctggat ctatttcaat
     8821 ctttaccttg caccctttcg tatgccggtt tttttcttta tatccggcta tttgattcgc
     8881 cgctatatcg acagcgtacc gtggggaaat tgtctcgata aacggatctg gaacatcttc
     8941 tgggtgctgg cgctttgggg cgtggtgcag tggctggcgt taagtgcgct gaaccagtgg
     9001 ctggcacctg agcgcgattt aagcaatgcc tccaatgccg cttatgccga ttccaccggt
     9061 gagttcctgc acgggatgat caccgccagc accagcttgt ggtatctgta tgcgttaatt
     9121 gtctatttcg tggtatgtaa aatttttagc cgtctggcgc tgccactatt cgccttgttt
     9181 gtactgctga gtgtggcggt taatttcgtt cccacgccgt ggtggggaat gaacagtgtg
     9241 atccgcaatt tgccttatta cagccttggc gcatggtttg gcgcaacaat aatgacctgt
     9301 gttaaagagg taccgttgcg ccgccatctg ctgatggctt ctttgcttac cgttctggcg
     9361 gtcggtgcct ggttgtttac tatctcgctg ctgttgtcgc tggtatcgat tgtggtgatc
     9421 atgaagctgt tttatcagta cgagcaacga ttcggtatgc gctccaccag cctgctgaat
     9481 gtgattggct ccaacaccat tgctatctac accacccatc gcattctggt tgaaatattc
     9541 agcttaactc tgcttgcgca aatgaacgca gcacgctggt cgccgcaagt cgaactgaca
     9601 ctcctgctgg tttatccctt tgtcagtttg ttcatctgta ctgttgcggg cttgctggta
     9661 agaaaacttt cacagcgtgc attcagcgat ctgttgttct ccccgccttc actgcccgct
     9721 gccgtcagtt actcccgcta agccatcgcc ccagccgggg cgattcaatt tgctaaacca
     9781 tgtcgattac ggataatgaa cagcattgct tatcagagat atgcccccat atgttgaggc
     9841 atatcctaac gagaatctga caaccgttat gcctgaacaa tatcgttata cgctgcccgt
     9901 caaagcgggt gagcagcgtc tgctgggcga gttaaccggc gcagcctgtg caacgctggt
     9961 agcggaaatt gccgaacgtc acgccggtcc ggtggtactc attgcaccag atatgcaaaa
    10021 tgctctgcgt ttgcatgatg aaatcagtca gttcaccgat cagatggtga tgaatctggc
    10081 ggactgggaa actcttccct acgacagctt ttcgcctcat caggacatta tctcctcgcg
    10141 cctttccacc ctttaccagc taccgacgat gcagcgtggc gtactgattg ttccggtgaa
    10201 tacgcttatg cagcgcgttt gcccacacag ttttctccac ggtcatgcgc tggtgatgaa
    10261 aaaaggtcag cgcctgtcac gagatgcatt acgaacccaa ctggacagcg ccggttatcg
    10321 ccatgttgac caggtgatgg agcacggcga atacgccacg cgcggcgcgt tgctggatct
    10381 cttcccgatg gggagtgagc tgccttatcg tcttgatttc tttgatgatg aaatcgacag
    10441 cctgcgggtg tttgacgtcg acagccagcg cacgctggag gaagtagaag cgatcaatct
    10501 gctgcccgcg cacgaatttc cgaccgataa agcggcaatt gaactgttcc gcagccagtg
    10561 gcgcgatacc ttcgaagtga agcgcgatcc ggaacatatt taccagcaag tgagtaaagg
    10621 cacattacct gccgggatcg agtactggca gccattgttc ttcagcgaac cactgccgcc
    10681 gctgttcagt tatttccctg ccaatacctt gctcgtgaat actggcgatc tggaaaccag
    10741 tgccgaacgt ttccaggctg acacgctggc gcgttttgag aatcgcggcg tcgatccgat
    10801 gcgcccgctg ttgccaccac aatcgctctg gctgcgggtg gacgagctct tctcagagct
    10861 gaaaaactgg ccccgggtgc agctaaaaac taaacattta ccgacaaaag ccgcgaatgc
    10921 caatttaggt ttccagaaac tgccagacct agccgttcag gcgcaacaaa aagcgccgct
    10981 ggatgcgctg cgtaagttcc tcgagacttt cgacggtccg gtggtgttct cggtagaaag
    11041 tgaaggtcgc cgtgaagcgc tgggtgaact gctcgcacga attaaaattg ctccgcaacg
    11101 cattatgcgt cttgatgaag ccagcgaccg tgggcgttat ctgatgattg gcgctgccga
    11161 acatggtttt gtcgataagg tgcgtaatct ggcgctgatc tgcgaaagcg atctgctcgg
    11221 tgaacgtgtt gcccgccgtc gtcaggattc tcgccgcacc atcaaccccg atacactgat
    11281 ccgtaacctt gcggagctgc atattggtca gccggtggtc catctggagc acggtgtcgg
    11341 gcgctatgcc ggaatgacca cgctcgaagc gggcggcatt actggcgagt atttgatgct
    11401 cacctatgcc aacgacgcca aactgtatgt tccggtgtcg tcactgcatc tgattagccg
    11461 ttacgcaggt ggcgcggaag aaaacgcccc gctgcataaa cttggcggcg atgcgtggtc
    11521 acgcgcgcgg cagaaagcgg cggaaaaagt gcgtgatgtg gcggcggaat tgctggatat
    11581 ctacgcgcaa cgcgccgcca aagagggctt cgcgtttaaa cacgatcgtg agcagtatca
    11641 gttgttctgc gacagcttcc cgtttgaaac cacgccggat caggcgcagg ccattaatgc
    11701 ggtacttagc gacatgtgtc agccgctggc aatggatcgt ctggtgtgcg gcgatgttgg
    11761 ctttggtaaa acagaagtgg cgatgcgcgc cgctttcctg gcagtagata accacaagca
    11821 ggtggcggtg ttggtgccta ccacccttct cgcgcagcag cattacgaca acttccgcga
    11881 ccgtttcgcc aactggccgg tacgtatcga aatgatctcc cgtttccgca gcgccaaaga
    11941 gcagacgcaa atccttgcgg aagtggcgga agggaaaatc gatattctga tcggtacgca
    12001 caaactgctg caaagtgacg tcaagtttaa agatttaggc ctgctgattg tcgatgaaga
    12061 acaccgcttc ggggtgcgtc ataaagagcg cattaaagcg atgcgcgcga acgtggatat
    12121 tctgacgctt actgcaacgc cgatcccacg cacgctgaat atggcaatga gcggaatgcg
    12181 tgacctgtcg attatcgcca cgccgcccgc ccgtcgtctg gcagttaaaa cctttgtccg
    12241 tgagtatgac agcctggtgg tccgggaggc gatcctgcgt gaaattttgc gcggggggca
    12301 ggtttattat ctctacaatg atgtggaaaa catccagaaa gctgccgaac ggctggcaga
    12361 actggtgcca gaagcgcgga tcgccatcgg tcacgggcag atgcgcgagc gcgaactgga
    12421 acgggtgatg aatgatttcc atcatcaacg tttcaacgtg ctggtttgta caaccattat
    12481 cgaaaccggg atcgacatcc cgacagccaa caccattatc attgaacgcg cggatcactt
    12541 cggtctggcg cagctgcacc agttacgcgg tcgcgtcgga cgttcacatc atcaggcata
    12601 tgcatggctg ctgacgccgc atccaaaagc gatgactacc gatgcacaaa aacgtcttga
    12661 agcgattgcc tcgctggaag atctcggtgc aggctttgcg ctggcaacgc acgatctgga
    12721 gatccgcggc gcgggtgaac tgcttggcga agaacaaagt ggctcaatgg aaaccatcgg
    12781 tttctcgctg tatatggagt tgctggaaaa cgccgtcgat gcactgaaag ccggacgcga
    12841 gccgtcgctg gaagatctca ccagccagca aacagaagtc gagctgcgga tgccgtcgct
    12901 attgccagat gatttcattc ctgacgtgaa tacgcgtttg tcgttctata aacgtattgc
    12961 cagcgccaaa acggaaaacg aactggaaga gatcaaagtc gagcttatcg atcgcttcgg
    13021 cctgctgccg gatccggcgc gtaccctgct ggatgttgcc cgtctgcgcc agcaagcgca
    13081 gaaactgggg atcaggaagc tggaaggtaa tgagaaaggc ggggtgatcg aatttgccga
    13141 gaagaatcac gttaatccgg cctggttgat tggtttgctg caaaaacagc cgcagcatta
    13201 ccgccttgat ggtccgacgc gcctgaaatt tattcaggat ttgagtgagc ggaaaacgcg
    13261 tatcgaatgg gtacgccagt ttatgcgtga actggaagag aacgcgatcg cttaatggta
    13321 aatcgggggc gtttctgcgc ccccatcggc aacatttaca aattctttgc acttccctgc
    13381 actatccgac actgtcacca tccataattc aggcttatct gtttattaca ataaccttat
    13441 atttattatg gatttttggt gatgatcaaa acgcgttttt ctcgctggct aacgtttttt
    13501 acgttcgccg ctgccgtggc gctggcgcta ccggcaaaag ccaacacctg gccgctgccg
    13561 ccagcgggca gtcgtctggt tgacgaaaac aaatttcatg tggtggaaaa tgacggtggt
    13621 tctctggaag ccatcgccaa aaaatataac gtcggctttc tcgctctgtt acaggctaac
    13681 cccggcgttg atccttacgt accgcgcgcg ggcagcgtgt taacgatccc gttgcaaacc
    13741 ctacttccag acgcgccgcg cgaaggcatt gtaatcaaca ttgcggagct gcgtctctat
    13801 tactacccgc cgggtaaaaa ttcggtaacc gtgtatccaa taggtattgg tcagttaggt
    13861 ggtgacacgc tgacaccgac aatggtgacc accgtttcag acaaacgtgc aaacccaacc
    13921 tggacgccaa cggcaaacac ccgcgcccgt tataaagcac agggaattga gttgcctgcg
    13981 gtagtgccgg ctggaccgga taacccaatg ggccatcatg cgattcgtct ggcggcctat
    14041 ggcggcgttt atttgcttca tggtacgaac gccgatttcg gcattggcat gcgggtaagt
    14101 tctggctgta ttcgtctgcg ggatgacgat atcaaaacac tctttagcca ggtcacccca
    14161 ggcaccaaag tgaatatcat caacactccg ataaaagtct ctgccgaacc aaacggtgcg
    14221 cgtctggttg aagtacatca gccgctgtca gagaagattg atgacgatcc gcagctgctg
    14281 ccaattacgc tgaatagcgc aatgcaatca tttaaagatg cagcacaaac tgacgctgaa
    14341 gtgatgcaac atgtgatgga tgtccgttcc gggatgccgg tggatgtccg ccgtcatcaa
    14401 gtgagcccac aaactctgta aatcctggat aaaaaaaatc cccgcgacac ggcatgtcgc
    14461 ggggaaaaaa tcagcagtag caggcattaa tgagggttaa tgcttattta taaattactg
    14521 ctgttccatg gagggtattc ggaccggtta cagaagtaat acggaaagat tttgcaccca
    14581 tctcatccgc tttttgcgcc agctgctctt ccagcgatcc cagatttgtc cccgctttag
    14641 cactgattgt accgactttt tgttggcctt ctggcgttga ctgaacttcg acagccgcaa
    14701 agctggcaaa tgacatggag cttaaaatcg ccgcagcgat gagggttttt acgtttttca
    14761 taatagtggc cttatgcaga tgaatgacgt cgcaagcaat tacttaacga tcgataacta
    14821 aatgacagat gtgatctgga tcacatacaa gatatttttt ataacaatca ttaattaatt
    14881 taaaaatctt taaatttcag ttaaatagaa agcaattatt tttatgggat ctggactggt
    14941 gaacggaggt gattattttt ataataatct ttcattcaca gtcaccaggt acaacggcat
    15001 catggcaact gactcaacac aatgtgtaaa aaaaagccgt ggccgcccaa aagtgttcga
    15061 cagggatgcc gcgcttgata aggccatgaa attgttctgg caacacggtt atgaagcgac
    15121 ttctcttgcg gacctcgtcg aagcgaccgg agccaaagcg cccacgctat acgcggaatt
    15181 taccaacaaa gaggggttat ttcgtgccgt tctcgaccgc tatatcgatc gttttgccgc
    15241 taagcatgaa gcacagctgt tttgtgaaga gaaaagcgtg gagtctgcgc tggctgacta
    15301 ttttgctgcc atcgccaact gctttaccag caaagacacc ccggcaggct gcttcatgat
    15361 caacaactgc accaccctct ccccagattc aggagatatc gccaatacgt tgaaatcacg
    15421 ccatgcgatg caagagcgca ctttgcagca gtttttatgt caacgacaag cgcgcgggga
    15481 aatcccgacc cactgtgacg tgacacatct ggcagaattc cttaattgta ttattcaggg
    15541 gatgtcgatc agcgcacgcg aaggtgcatc gctggaaaaa ctaatgcaga ttgccggaac
    15601 gactttgcgt ttatggcccg aactggtgaa ataagtcgca aaaaaagccc ctcagcctga
    15661 gcgaatgagg ggccaaattg cagagtacag ccttgttata ctttgttatt caaaatcagt
    15721 tgcccattac tatctagcgg gatctgcgta cccggatcgc ggtccatgcg gattttgccc
    15781 tgctgatcgc caatcttata ggtcacatca taaccgagca ttttttctga cttgtcatac
    15841 accgttttac aacgctgttg cgtagtcgtg taagtatcgc tttcctggag agagccctgg
    15901 atctggttac cggcatatcc accacccagc gcccccacaa cagtggcgac atctttaccg
    15961 cgaccaccgc caaactgatg ccctatcacg ccgccagcaa cagcgccgag caccgacccg
    16021 gtaatgcgat tttcatcctg caccggtcga cgatgggtca ctgtgacgtt gcgacactcc
    16081 tgacgcggtg ttttaaccgt ttccttgatt ggggttgcag aaacaacctg agcgtattgc
    16141 gggccacgtt caaacacgtt cagactggcc actgccgcta cgcccagcgc agctgcgaca
    16201 ccaatcccga tacccgccaa cattgattta ttcacgggaa cacctccttc tttgcagtta
    16261 tgccaatcat gcaagcgaag gagaaaagca gctatcagac agaagatgaa aagcgcggga
    16321 atggctggaa atacttaatg attcggagga gtctgaagga atgcctgatg cgacgcttaa
    16381 cgcgtcttat caggcctacc gctcgtacca tttgtccagg gcggatgagg cgtttatgcc
    16441 acatccgcca gtgtacgtcg attaatgcaa cttcaaacgc ggacggataa cgcggttaat
    16501 actccccacc agcatcatta atccggtttt aaagtaacca tgcagcgcaa tctgatgcat
    16561 tcggtatagc gagatatata caaagcgcgc aattcgtcct tcaatcatca ttgagccgcg
    16621 cgtcaagtta cccatcaggc taccaacggt ggagaagttc gacagcgata ccagtgaacc
    16681 atgatcttta tactgataat ttttcagcgg cttaccgttt atctgcgcca gaatattgtt
    16741 cattgcgcaa gtcgccatct ggtgtgcagc ctgagcacgc ggcggaacaa agcccccttc
    16801 cggacgcgtg catgacgcgc agtcgccaat agcgtaaatg tctggatcgt gggtggtttg
    16861 cagcgtcggt tccaccacca gctggttgat acggttagtt tcaagaccac cgatatcttt
    16921 caggaagtct ggcgctttga tcccggctgc ccacaccatc agatcagcct caatatattc
    16981 accatcttta gtgtgcaggc cgccttcatc agcactggtg accatggttt gcgtcagcac
    17041 gcgaacgcca agtttcgtta actcactatg ggccgcagca gagatgcgtg gcggtaatgc
    17101 aggcaaaata cgttctcccg cttctactag cgttacgttc agggcttcgt tggtcagacc
    17161 tttataaccg tagctgtgca gttgcttgac cgcgttatgc aactcagcgg agagttctac
    17221 acccgtcgcg ccgccaccga caatcgcaat gttcactttg ccattcgcgc ccaggttggc
    17281 ggagtatttc aggaacagat tcagcatctc ctggtggaag cgacgcgcct ggtgcgggtt
    17341 atcgaggaaa atgcagttct ctttgacgcc tggcgtattg aaatcgttag aggtgctacc
    17401 cagcgccatg accagggtat cataggcgat tttacgttcc ggaaccagca gttcaccttt
    17461 ctcgtcgcgt agttctgcaa tagtgattgt tttcgcttca cgatcaatat caatgacgga
    17521 acccaactgg aactggaaac catgattgcg cgcatgggcc agatagctca acgcatcgac
    17581 gccttcatca agcgagccag tcgccacttc gtgcagcagc ggtttccaca ggtggctgtg
    17641 gttacgatcg accagcgtaa ttttggcttt tttcttacgt cccagcttat gccccagctg
    17701 tgttgccatt tccagcccac cagcaccgcc gccgacaatc acaatctttt tcaatggcgt
    17761 agtcaacgtg acccccttaa atttattaac caattgttaa ttaatggtta ttaacatagc
    17821 tcttaattaa caacaggtta cagcaatgaa aatgttcttc gctattgaga ataacatgaa
    17881 tggtgcattg gtcataccaa aattgatgtt aatcaagttt tgttgctgaa aagataggcg
    17941 actttaccgg gtgagatcaa aaaatagagt ttattgttta ttatttgagc gaatttgtga
    18001 gagtgatata gagtgagggg tgtatgcagg gcgttcggta ggcctgataa gaggcgcatc
    18061 aggcattgat gctcgactgt cggatgcggc gtaaacgcct tatccgactt acagaattcg
    18121 gagcgcatca ggtaccggca aggttacctg atgcgacgag ggcatttacc cgagggtttt
    18181 gaacgcttta atgcgctgca aatgcgggga gatattcttg aatttgtgcg tctgctcttc
    18241 gtcccagaca atctcgtaat aatgatgcaa ctcttcagat gtccgctggc tgttaagcgc
    18301 ttcatcatta cgcgacaaaa tcaccaggca acgatcgcga ttcttctcgc ggaagttggt
    18361 cacacactta gtggcaatat cggcatactc ttccgggcga tcaatcttgc cttccatgtt
    18421 ctcataaggg aacaaattag ggttgaagat cacctggcgg atatcgcaaa gaaaaccaat
    18481 ccgttccgcc cagtatccgc ccagaccaac gccgcaaatc agcggacgct cgtcgacgtt
    18541 cagttgcaac attttgtcca cttctttaag cagatgctgc atatcatgtt tcggatgccg
    18601 cgtactgtag cttatcaagc gtacatccgg gtcaataaac tgcaattgta agactttctc
    18661 gtggttaccc ggactgttag agtcaaaacc gtgtaaatag ataatcatcg cttcctcaca
    18721 taagccaggg ttagtgacct gctttctctt cctgccagcg ttcatgtaac tgattcagac
    18781 gggtgctgct cgccttccag cgagccgagt ccatcagttc ctgtcgcgaa aatgaacctt
    18841 tatgatacaa acgtgtaaca cgttctgcct tgatcggtga cagattatct aacacgctga
    18901 cggccccttt acgattattg cagaccagga tcatatcgca acctgcgtcc agtgatgcct
    18961 gcccgcgttc ggcataactg cccataatcg cggcaccttc catcgataaa tcgtcagaga
    19021 aaattacacc gtcaaaacac agttcctgac gcaaaacggt tttcagccag taagaagaac
    19081 cgctcgccgg acgcggatca acatcactgt agatcacatg cgcaggcata atggcgtcga
    19141 gtttattttc gcggattaac gtgctgaaaa ccgacatatc tttggcacga atttccgctt
    19201 gcgggcgtgg gtcgcacggc gtctctttgt gtgaatctgc agttactgca ccgtgtcctg
    19261 ggaagtgttt cccggtcgtt ttcattccgg cttcatgcat cccatcaata aaccgactag
    19321 cgattgccag ggctttttct gggtcggcat gataagagcg ctcgccaatc gccgcgctga
    19381 tatgtcctac atccagcact ggcgcaaagc tgatatcaat atccatagcg atcatttcgc
    19441 tggccatcag ccaacccgcc tcttgcgcca gtttgccgcc ctcttccatt cccaacagcg
    19501 cagcaaagga ttgtgctgcc ggtaagcggg taaaaccttc gcgaaaacgc tgcacgcgtc
    19561 caccttcctg atctaccgcc accaccagat gattgcgcga tgctgcgcgg atctggcgca
    19621 ccagttcacg taactgggca ggatcatgat agttacgcgt aaagagaatc agccctccca
    19681 ccagcggatg cgccagtatt tcacgctctt ccgcgtccag ttcataacct tcgacatcca
    19741 acattactgg acccacactg ctctccttat tgttttatta atagctgccg ccaggtgtca
    19801 tcggccagcc tgataaattg ttgatcgccg gtttgtcgcc agcggtactc aaaccaccct
    19861 gctttgagca tcagcagcca gggaaaccat cgcctgacct gacgccataa ttgcgccgga
    19921 taaatcttcg cgcgagtggc atagtcattg accaattgcc ggtgctgttc agtattttcc
    19981 acccacaccg ccgccagttc cagcgcggta tcaccatctc cggcatactc ccagtcgatg
    20041 agttttaacc ctgatgcgct atgcactaaa tttccggcgt ggacatccat atgcaatgga
    20101 cttaagcgta aaggtcgtgg ttcccgcgct ttgcgcagac gttttaacat tcgcagccaa
    20161 cccacagtcc gccgcgccgg atcgctttgc tgccagtaca gttccagtaa cggcaacagc
    20221 gttattcgcc agccaaaacg tggttgttga tgtagataat acagcaagcc tgccagttcg
    20281 ttggtatccg gcaaatacgt ttttaccgcg ccgggcagat agtcgactac catccagtca
    20341 cggagatata aatgcggctt cggtgcaatg cttgcgggta gttgtgataa agcccgatac
    20401 tggcgcaaga acgcggactg cggcgcatca ggatcgtgcg gctgacgcac aacaaaacgc
    20461 tggccctgat gttcaatgag aaaactaccg ccactaagcc cactattaaa cgtcgtgacg
    20521 ggatgaaact gcgggaaaaa gcgcgacagc aattcgtcgc gcgtgatggg attattactg
    20581 cgaaacggca cctttacctg accagataat ttcgcccgtc tgcaccagca tcagctgcat
    20641 ttgtagggtc ggagcgttaa cgttgccaga ggcgctggag tacagcacgt aatgagcgcc
    20701 gacattgcgg gcaatgccta tggctttact acgggtgcct aaactgtcct gcggcgacaa
    20761 acctaactgt tgcttagcca tcgacagttg ctgggcggaa accagggtaa atttcccgtt
    20821 attagccagc gcatttcgca gcgtttcggt cgcttctgcg gcattcagcg aaccgttagt
    20881 acggttgtta acgctatcaa ccagcaggac gctacccgca gtcaccccgt cagccccaag
    20941 catcttactg actatcggct gcattgcgcc attccagtca taatggcgaa tatgcggcgc
    21001 aggcggtgcc gttcgatctt cgtgctcaat tgggcctggc tgctgcggga tcgtcggcac
    21061 cgagggcact gtggggacag gctgttgtgg ctcggctggt tgttccggcg ctggtttcac
    21121 ttcttctacc ggtgcaggtt cacgttgccc cacacacccg gcgagaaaca tcgccagcgc
    21181 ggtaatcaag gcgtagcgac tcatttttgt catcaagatt caccccttac aaatatagat
    21241 aaagtctgac tttgtgcgcc cccagaaaat tggcgctgcc gtacagcgtt accgccgaat
    21301 gtgcgggaat ggtaacgctg cgtggccttt ccagaggatg catctccagc cctctggcgt
    21361 cataccagta aaaacgataa tgaacggtaa cgggtttttg cctttcgtta taaagcgttg
    21421 aggatgctga aggttgaata tcagacgtcg aaaggacggg cttttctgca ctgatgcccg
    21481 cagccaataa agatgactcc atcaccagcg attgctcatc attcaccgga atttccggat
    21541 gtgaacgaca gcccaccagc agtaacaaca ccagagacac cagcccaaag catccttttc
    21601 tcatcgttac agacctttat gcgccagcat tggtcccagc ggacggccac ccaacaagtg
    21661 catatggatg tggtaaacct cttgtccgcc atggcggttg gtattcatga tcagacgata
    21721 gccatcttcg gcaatacctt cttgctcagc aatttttgcc actacggtga tcatgcgtcc
    21781 cagcgcctgc tcatgctcag ctgagacgtc gttcacagtc ggaatgagga tattcggaat
    21841 gatcagaata tgcgttggcg cttgcggcga aatatcgcga aacgccgtta ccagatcatc
    21901 ctggtagacg atatcggagg ggatctcacg acgaataatt ttgctgaata tagtttcttc
    21961 tgccacgaca ttttcctttt tcaaatagcc atgcgttgcg ccatgctacg ccgcgccaca
    22021 ctgcgagtat agagtatggt cgagttaaac ctgctctttc aaccttaact cacgatttct
    22081 taagcaaaaa atcgttttgc tgccgtatat atcgccatta ttcccatttc tgcctgcgaa
    22141 tgcgctgaaa agacattctc tgtttggttt acacgacaaa tatgaatgcg tatatttctc
    22201 atttgcattt acaaacaaaa ttattcgcac actaaatata atcgctcatc accctcctcc
    22261 ggcatgagcc tgacgacaac ataaaccatg agatttcaaa tgctttcaac acaatttaac
    22321 agggataatc aatatcaagc catcaccaaa ccgtcactac ttgccggttg catagcactg
    22381 gcactattac cttctgccgc ttttgctgca ccagccactg aagaaacggt gattgttgag
    22441 ggttcagcca cagctccaga tgatggc
//
LOCUS       DADLFA010000056        22278 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000056 DADLFA010000000
VERSION     DADLFA010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22278
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.056"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(18..93)
                     /locus_tag="JQH65_001787"
     tRNA            complement(18..93)
                     /locus_tag="JQH65_001787"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:His,seq:gtg)
     gene            complement(152..228)
                     /locus_tag="JQH65_001788"
     tRNA            complement(152..228)
                     /locus_tag="JQH65_001788"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(192..194),aa:Arg,seq:ccg)
     gene            complement(331..1716)
                     /locus_tag="JQH65_001789"
     CDS             complement(331..1716)
                     /locus_tag="JQH65_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY6139228.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAVGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNESGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(1907..2647)
                     /gene="rffM"
                     /locus_tag="JQH65_001790"
     CDS             complement(1907..2647)
                     /gene="rffM"
                     /locus_tag="JQH65_001790"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HAY6139229.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(2650..4002)
                     /gene="wzyE"
                     /locus_tag="JQH65_001791"
     CDS             complement(2650..4002)
                     /gene="wzyE"
                     /locus_tag="JQH65_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen assembly polymerase"
                     /protein_id="HAY6139230.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTREVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(3999..5078)
                     /gene="rffT"
                     /locus_tag="JQH65_001792"
                     /gene_synonym="wecF"
     CDS             complement(3999..5078)
                     /gene="rffT"
                     /locus_tag="JQH65_001792"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HAY6139231.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRVLAIAAGEVA"
     gene            complement(5075..6325)
                     /gene="wzxE"
                     /locus_tag="JQH65_001793"
     CDS             complement(5075..6325)
                     /gene="wzxE"
                     /locus_tag="JQH65_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HAY6139232.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(6327..7457)
                     /gene="rffA"
                     /locus_tag="JQH65_001794"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(6327..7457)
                     /gene="rffA"
                     /locus_tag="JQH65_001794"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HAY6139233.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(7462..8136)
                     /locus_tag="JQH65_001795"
     CDS             complement(7462..8136)
                     /locus_tag="JQH65_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-fucosamine acetyltransferase"
                     /protein_id="HAY6139234.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(8114..8995)
                     /gene="rfbA"
                     /locus_tag="JQH65_001796"
     CDS             complement(8114..8995)
                     /gene="rfbA"
                     /locus_tag="JQH65_001796"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAY6139235.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLVQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDQERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(9014..10081)
                     /gene="rffG"
                     /locus_tag="JQH65_001797"
     CDS             complement(9014..10081)
                     /gene="rffG"
                     /locus_tag="JQH65_001797"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAY6139236.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSDRFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(10081..11343)
                     /gene="wecC"
                     /locus_tag="JQH65_001798"
     CDS             complement(10081..11343)
                     /gene="wecC"
                     /locus_tag="JQH65_001798"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HAY6139237.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(11340..12470)
                     /gene="wecB"
                     /locus_tag="JQH65_001799"
     CDS             complement(11340..12470)
                     /gene="wecB"
                     /locus_tag="JQH65_001799"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HAY6139238.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKAPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELVANYPFIDPNKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(12526..13572)
                     /gene="wzzE"
                     /locus_tag="JQH65_001800"
     CDS             complement(12526..13572)
                     /gene="wzzE"
                     /locus_tag="JQH65_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HAY6139239.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(13584..14687)
                     /gene="wecA"
                     /locus_tag="JQH65_001801"
     CDS             complement(13584..14687)
                     /gene="wecA"
                     /locus_tag="JQH65_001801"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HAY6139240.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMALGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(14927..16186)
                     /gene="rho"
                     /locus_tag="JQH65_001802"
     CDS             complement(14927..16186)
                     /gene="rho"
                     /locus_tag="JQH65_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAY6139241.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(16271..16372)
                     /gene="rhoL"
                     /locus_tag="JQH65_001803"
     CDS             complement(16271..16372)
                     /gene="rhoL"
                     /locus_tag="JQH65_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide rhoL"
                     /protein_id="HAY6139242.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(16513..16842)
                     /gene="trxA"
                     /locus_tag="JQH65_001804"
     CDS             complement(16513..16842)
                     /gene="trxA"
                     /locus_tag="JQH65_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HAY6139243.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            16973..18238
                     /gene="rhlB"
                     /locus_tag="JQH65_001805"
     CDS             16973..18238
                     /gene="rhlB"
                     /locus_tag="JQH65_001805"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HAY6139244.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEVLEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            18372..19856
                     /gene="gppA"
                     /locus_tag="JQH65_001806"
     CDS             18372..19856
                     /gene="gppA"
                     /locus_tag="JQH65_001806"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HAY6139245.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(19903..21924)
                     /gene="rep"
                     /locus_tag="JQH65_001807"
     CDS             complement(19903..21924)
                     /gene="rep"
                     /locus_tag="JQH65_001807"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HAY6139246.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKYYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
BASE COUNT         5593 a         6108 c         5543 g         5034 t
ORIGIN      
        1 gtcacaactt ctaataatgg ggtggctaat gggattcgaa cccacgacaa ctggaatcac
       61 aatccagggc tctaccaact gagctatagc caccactaca aatcttgtta cgcggtatta
      121 ctaccaccgc agctcaagcg ccgggactaa atggcgcgcc cgacaggatt cgaacctgag
      181 acctctgcct ccggagggca gcgctctatc cagctgagct acgggcgctt agcgccgttg
      241 cgggggtgga taatacggac ttcccacccc tctgtctagt ccctttttaa ataaattgcg
      301 cgtttggtta cggtttgtgc gttttgctgc ttattcctcc agtttatgcg ctttcccgtg
      361 gcgattaagg ccaaaaactt tataaatcgc cgtcaccgcc agcataaaga tgatgccaac
      421 aaacagcgac atacgcgtat cttcattaaa gtacatgccg atcaaaacgc aaatcaggaa
      481 tgccattgtt acgtagttgg cccacgggaa caggattgag cggaacggat ggctggcaat
      541 cgccgcttta tgtgcacgcc gaaaacgcag ctggcttatc aatatcacaa accatggcac
      601 catccccgga agcacgctgg cactgtagac gtagacaaac acacgctgcg gattgggaat
      661 gatgtagttc aggcatgagc caattagcag aatagcaata gatactgcca cacccgcaac
      721 tggtacgccg tgacgggaaa ctttcgccat cgccgccggt aactgacggt ttttcgccag
      781 tgcgtagagc atacgtccgc aactgtacat gccgctgtta cagccagaga gcgcagccgt
      841 cagcaccaca aagttgataa tgcccgccgc tgcggtaata ccgattttgg caaaagtcag
      901 tacgaacggg ctgccgttgc tgccactttc attccacggg aagatggtga cgataacgaa
      961 aatcgcgcct acgtagaaaa tcaggatccg ccacagcact ttgcctacgg cactgcgcaa
     1021 cgtcacctgc ggattcttcg cttcaccggc agtaatgcca atcagctcca cgccctggta
     1081 ggacgccacc acaatacaca gcgcggtcag gaaccctttc cagccacccg caaagaaacc
     1141 gccatgctct gtgagattgc taaaaccaat cgactgcccg ccattgccaa agccaaagaa
     1201 aatcacgccc aggccaatga caatcatcac gataatcgtg gtgactttga tcatcgcgaa
     1261 ccagaactcg atttcgccgt acaaccgcac cgccgccaga ttcgccaacg ccaccagcgc
     1321 cactgcgatc aatgcgggta tccactgcgc catctccggg aaccagaact ggacataaac
     1381 gccaacggcg gtgatttctg agatccccac cgccatccac ataaaccaat aagaccaggc
     1441 ggtgagataa ccaaagaacg ggctcatata acgatgcgca taaacggcga acgaaccggt
     1501 aaccggttcg aggaacaaca tttcgcccat tgaacgcatg atgaaaaaga cgaatagccc
     1561 ggcgatgata taggccaaca atacggatgg cccggcccat ttcaaggtac tggcggcccc
     1621 cataaacagg ccgacgccaa tggtgccccc cagggcgatg agttcgatat gtcgagcttc
     1681 cagcccacgc tgtagctctg gtttgttatc tgccataaat cctcgtgttg tgtttgcatg
     1741 ctttccggtg ttaccggtta tcgttatggg tacatcgagt gttgcgaatg ttttcgtaat
     1801 tcaggagaaa tggcaaataa agcattaaaa atttgaatgc tttgtgtaat aaaaaagcag
     1861 acaggcgacg gagtgaccac tccgtcgctt tacaaagaga ggaaaatcag aggttgccgg
     1921 tgtagtgcca gcgtaaataa cgcagcaaac gaagctgacg cttaatgcgg ctcggctgcg
     1981 aaagcaggcg gtagagccac tccagtccca gcgtttgcca gattttcggt gcgcgtttta
     2041 cgtgaccggt gaaaacatcg taagtcccgc caacgcccat atacagcgca tctggatgca
     2101 ccagacggca gtcgcgcatg aagatctcct gctttggcga tcccatcgca acggtgacga
     2161 tttgcgctcc gctggcatga atgcgttcaa acagcgcctg acgctgctcg ggtttaaaat
     2221 aaccatcctg actgccaacg atattcacat tccactggtt gcgcagttta gcttcagttt
     2281 gcgccagcac ttcaggttta ccgcccacaa gaaataccgg cgtcccttct ttgcctgcgc
     2341 gcgccatcag ctcttcccag agatcggcac cggcaacgcg ggaaacctgc gcctgcgggt
     2401 actttttacg tactgaacgt acaacgctga tgccatccgc atatttaaat tcggcagcgt
     2461 taattaactc cctgacctcg gcgttatctt caatggtcag cattttttca gcattaatgg
     2521 caaccagcgt tccctgctta agctgcccgt cagcaaacag ataatcgagg gcgtgctgca
     2581 tatcacgcca accaatcaac tgtaagccac gcagcgtata ggtcggtgcc gtggtgttgt
     2641 tattcattgt tatccttcaa cctgcgtccg gagcgatgat tttgtacgtt tatgaatgag
     2701 tccggcgctt tcaaaaagcc agtacaacag ttttgcgatc atcagacatg cgccgaagac
     2761 cacgataaaa aagactacgc gtgagacaaa cgaatccagc ccttcacgtg ccagcacgat
     2821 catattgaag atcgccccaa agcagaaact gtgcaatatc gcagccttat agcgattagg
     2881 ctcgcggttg cccagctcat acagccagtc gaaccatttg atgatcagac caaccacgat
     2941 cgccccgagc gggatgaaca acgcgccgcc catcaccacc agtgagccta taagcgtagg
     3001 cgagatcgcc agtccggagt ggttattcag cacttccctg gtaaagtagt tggctgagtt
     3061 cagcaccata ctcgggcgac ccggccacag ccaggaaggg ataaagacat agaaatcgcg
     3121 gacaattgga gccaggccct ggaagtcgat gttgtcgtag ttctgcaaca gcaacgccag
     3181 attctcccac ggcgagaagg tgtcgcgagt gagatagaga aacgtataga acgcttcatc
     3241 gccgctcaca ttcattccat agcgttttag cgccagccag aacatgccca caatccccag
     3301 cacgcccgcc gccgccagca tccacaacga aatccagccg cgaataatgc caataaacag
     3361 gaagatagcg aatgcgatga tgatattggc gcgagtgccg ccgacaatca tataagtcag
     3421 caagccaaag gcgaccgtgc tgacgaggaa aaacagccac gctttgctat cctggcgcag
     3481 aaagtagacc accagcatcg ccgggatgaa aaagtaaaag aagcgtttta acgccacgcc
     3541 ggagacttca ctggagaaga tctgactgta ggagttaagc cggaacagca aaaagccgtt
     3601 gtgcatgaag aagatgccga cgcttaccag cgcgatgccc atcaggatca cccacgtaag
     3661 attggtctcc acgcggttca tggtaaacag cggacggcgc ggtacatcag caacgcgttt
     3721 gcgtaggcgg gttttgtagg tgacatagta aaccgcgtag aagcagcccg cagaaagcaa
     3781 cgcctgcaac aagatttctg gcggcgcgac accaacatca aagcgaaata ccagcacgct
     3841 ggtcagcggg aagccgaaga aaaaggtgag caaaaacagc aatgaaaaga agacattgaa
     3901 gttaaagcgc acacggcgaa actcaaacca agtcagcgtg gcaataaaca gcgtgcagag
     3961 cagccagacc acaaacaggc cactgaattg cagcagactc atgcgacctc cccggcggca
     4021 atcgccaaca cccgctgcca gccttgtaga tagttagggc taaagaaggc aatggtgttt
     4081 ttatccaccg acgccaactg gcgctgcgct tcacgcacaa tatcctcgtt gagatcgtcg
     4141 gtagtaaaca gcaccggcaa atgctgttcc gtcatatcct gccagaacgg attttcccgg
     4201 ttaagcacac aaggaatgcc cgcctgaatc agtaagcaaa gcgtaccaat gccctgctgg
     4261 cgggcaaaaa taaagtaacc aagatcgcac tgacgaagta gcgccagata ggcgtcaaat
     4321 tccagttttt cgctcaaaat ttgcagattt tcttcgctga ataactccag ccccgcctga
     4381 cgaacttcct caatgtacgc ttcgttatta ggcggatatc ccatcggcac caccactttt
     4441 accgtatcgc caaattgctg atgaacggcg cgcaaggcag caatatggtc attgctgcgg
     4501 tcaccggagt tccccaccag aatggtcatt ttcccttcac gttgccgatc gttcgccatc
     4561 gtattgagag aagggtccat ccgcgtcggg aagtacagca gttcgccccg cacctttgga
     4621 tgcgttttgg caaaaaagct caaatcaccg cgggtggcaa atacacagcc gactcgcttt
     4681 tgcgccaggc gacgtagcgg gtaaaaaagc ttatatctca agccactgga aagctcatac
     4741 aggtctgccc cccagatatg ccagaaaaac tggctgggct taatgccacc actcagcaga
     4801 gccagccaca gtgtgggatt gaactgaccg tggaaaaaaa atcgctgctg acggttagct
     4861 tttgctttcg cgatgaccgc ttccgccagc gattttttcc cagggaaaaa ttgcacagaa
     4921 agcgccggac agctatcact taagccgtcg tccttgccaa caaccataaa ctcgcgcgcg
     4981 tgctcgctcg tcgcggccag cgcgtcattg aaaaaccgca aaacggttcg gttatggtga
     5041 gggatatccg atcccagtac gtgaatcagt acagtcatgc ccgcctacgc cagagtaaaa
     5101 acacgccaca acaaagagaa aaatagacga tataagttgc catatatgcc tgcgccgcgc
     5161 ccagtgcacc atgcgcaggg attagccagt gggcaaatac catcaataaa gtgaactggc
     5221 tgacttccgc cagaatataa aaccgcagtg acgctttggc gatcaccaga taaccaaaga
     5281 cataagcgcc cacttttagc acatcaccca ctaactgcca ggcaaagaga tcgcgcatag
     5341 cggtaaattt attcgacaac agcaaccaga tagcaaaatc acgcagcaac cagacggtaa
     5401 aactcgccgc cgccactgcc ggtaagacga attttagcga tttaaccact tcccgggtga
     5461 tatcgcgctt ttccgttagc cgcgacaacg tgggcagcaa atatacgctg aacgatgccg
     5521 taatgaattg caggtaggca tcggaaatac tgctcacccc ttgccagatc cccacttcat
     5581 cccagctata ctgcgccgcc agcagtttac gcatcatgat gtaagcaaca ggcaaggtca
     5641 ccgacgtaat caacgccatg agcgtaaatt tgctcaactg ccctgccaga ccgttatccc
     5701 agctgggttt cagatagctt aacgggatga caccacgttt gatcaacatg atggcggcag
     5761 gaattaccac cagcgcggga atcagcgcca gacccagcaa cgccccttca taaccgccca
     5821 aacggtaact gacgtagtac gcgagaacgc caatcaagct gccgacaatc agcgataacg
     5881 cattacctgc ggcatcgcga aagcctttca tcagcgccag taacaggttg ccccaggcga
     5941 tccccatttg caccagcgcc actaaacgca ccagcccctg atagtcggta ttaccaaaca
     6001 atccctggct gattggcgca gctgccagca caaaaaccag cgccatcagc gtagagaaac
     6061 caagtaccat cgctgatgaa gtgccgacca cgcggcgcag ctgttgcgga ttatcatggt
     6121 actgggcaac gtatttggtt acaccgttaa agatgccagc cccggcaagc acgccgagca
     6181 cggtaatcaa ctggcggaaa tttgccgcca gcccaagccc cgccggacca aatgacaccg
     6241 ccagcaactt accgaccagt aacccggcac caatcttgac cagtgtactg gccgccgtcc
     6301 acaaggacgc ttttgccaac gacatatcag gagaagtagt tcaacaaagt cgcaattacc
     6361 gtacgctgat tgacgggcga caggttgtag aacagcggca ggcgcagcag gcgctcgctc
     6421 tctttggtgg tgtagcgatc ttcaccgtgg aactcaccaa agtgttcccc cgcagggcaa
     6481 ccgtgcagcg gaatgtaatg aaacaccgcc atgatttccg cttctttcag aaagttaatc
     6541 aacgcgctcc ggtcatcaat atcccgcagt ttaatgtaga acatatgcgc gttctgcacg
     6601 cagccatcag gaatcgacgg cagctcgata cgcccggctt tcgccagagg cgctaacgca
     6661 tcgtagtaat tttgccacag cgccagacgt tgctggttga tacgatccgc tgcttccagt
     6721 tgcgcccaca ggtatgcagc ttgcagatcg gacatcaaat agctggagcc aatatcgcgc
     6781 caggtatatt tatcgacctg accacggaag aactggctgc ggttagtgcc cttttcacgg
     6841 atgatctcgg ctcgttcgat taacgcttta tcgttaatca gcgtcgcgcc gccttcacca
     6901 cccgccgtgt agtttttggt ttcatggaag ctaaagcagc caatatgacc aatggttccc
     6961 agtgcacgcc ctttgtaagt ggacatcacg ccctgagcgg catcttccac cacaaacaaa
     7021 ttatgctttt tcgccaacgc cataatggtg tccatttcgc aggccacacc cgcgtaatgg
     7081 accggcacga taacgcgcgt tttgtcggtg atcgctgctt caatcaacgt ttcgtcgatg
     7141 ttcatggtgt ccgggcgaac atccacaaag acgattttcg cgccacgcag cacaaaggca
     7201 ttggcggtgg agacaaaggt gtagctcggc atgatcactt catcgccagg ctggatatcg
     7261 agcagcagcg ccgccatctc cagcgaagcg gtgcaggacg gcgtcagtaa cactttggcg
     7321 ctgccaaaac gttgctccag ccactgctgg cagcgacggg taaaaccgcc atcgccacac
     7381 agtttgccgc tacccattgc cgactgcata tagtcgagtt cggttcccac caccggcggt
     7441 gcgttaaatg gaatcatgtg atcacctgta taaccagtac gcggtgcttt ctacattcgc
     7501 accactttgt atgtatcgtt taagcgcggc ggtgttgccc atttgggtcg ccacccgcaa
     7561 agttgtttta ccgcgagcat acgcccagtt tagcgccgtt tgcatcagct cagcacctgc
     7621 accgcgtcca gccagcaggc caattcgcgc atctgtcgca ttgagttccc gtaaagagac
     7681 atagccgcga atatcgccgg acgccgcacg tagaatcaga cattgatgat caaaggtgcc
     7741 gcgcacggca ttttcaatcc actgtgcata aaagcgaccg ctggcgtcag gcgcatacca
     7801 cggcgcacga aaacggcttt gcgcaaatgc ggcgctagct aactgacgta atgcgggaat
     7861 atcggtctct tgtgccacga cagcaccgct atcactgaca ttgttcacgg gtagcgccaa
     7921 atcaacttca ccttctacca gggagaatcc cagctgttgc agggcatcca gttcacccgt
     7981 atttgatgcc gcaattttgg cctgcacccg tgaccacggc gctaacgcgt ctggcgtcag
     8041 gagcggtgct tcagacgtaa tgcgcacgat ggcgctgtta acaccaaaga aggcgttttc
     8101 ccaggttagt ggctcaatac tggcgcggac gggcacgaag taactccagc agatattggc
     8161 cgtagccagt tttcgctaat gaactggcag cacgcttcac accctcgtca tcgagccagc
     8221 cgttacgcca ggcgatctct tccaggcagg caatcttaaa gccctggcgt ttttctactg
     8281 tctgcacaaa ggtactggct tcaatcaggc tgtcgtgagt gccagtgtcc agccaggcaa
     8341 atccgcgccc gagcagttca acggtcaggt tgcccgcctc gaggtacatc tggttgatgg
     8401 aggtaatctc cagttcacca cgctccgacg gcttcacctg ctttgcgtac tccacgactt
     8461 tactgtcgta gaaataaagc ccggtcaccg cccagtttga cttcggctgt tttggctttt
     8521 cttccagcga gatagcgcgg aaattgtcgt caaactccac cacgccaaag cgttcctggt
     8581 ccatcacctg atagccaaat accgtcgccc cttccgtgcg cgccgcaaca tgacgcagct
     8641 tcggactgaa gccctgaccg aagaagatgt tatcgcccag caccagacaa gaaggttcac
     8701 cgttgaggaa ggtttcaccg atgataaagg cctgcaccag accgtccggg ctgggctgtt
     8761 cggcgtattc cagctggata ccgaactcac taccatcgcc cagcaggcgc tggaaataac
     8821 ctttatcttc cggcgtagtg atgatgagaa tttcgcggat accggccaac atcagcaccg
     8881 acagcggata gtaaatcatt ggcttatcgt aaatcggcaa cagttgcttc gatacgccgc
     8941 gcgtaatcgg atgcaatcgg gtgccggaac cgcccgccag gataatacct ttcatgtttg
     9001 cctccgctga aaattagcct ttcagaccta aacgctcgcc ctgatagctg ccgtcctgca
     9061 cctgcttcca ccagctttca ttagccagat accactgcac cgttttacgc attccacttt
     9121 caaaggtttc ctgtggcagc cagccaagtt cacgggcaat tttcgaagca tcaatggcat
     9181 aacgcagatc atgccccgga cggtcagcga caaaggtgat caagtcacga tagtgcacca
     9241 cgccgtgcgg cttgttcgga gccagttctt ccagcagctc gcaaatggtt tccacaacat
     9301 cgagattctt acgctcgttg tgaccaccaa tattataggt ttcaccgact ttcccggtgg
     9361 tcgccacgca atacagcgcg cgggcgtgat cttccacata cagccagtca cggatttgct
     9421 gcccgttgcc atataccggc agcggtttac ccgccagcgc gttgaggatc atcagcggga
     9481 tcagtttttc cggaaagtgg taaggaccgt agttattcga gcagttggtg ataagcgtcg
     9541 gcagaccgta ggtccgcagc caggcgcgca ccagatggtc gctgctggct tttgacgcgg
     9601 aataagggct gctcggcgca tacggcgtgg tttcggtgaa gaaatcatcc gtcgagtgca
     9661 ggtcaccata tacttcgtcg gtggagatat gatgaaaacg gaacgctgat tttttatctt
     9721 ccgtcagcgt attccagtaa gcccgcgccg cttcaagcaa tgtataagtc ccgacaatgt
     9781 tggtttcaat aaacgctgcc gggccgtcaa tagaacggtc aacatggctt tcggctgcca
     9841 gatgcatgac acagtctggc tgatgctcag tgaatacgcg tgccagttct gcccgatcgc
     9901 agatatcaac tttctcaaag gcaaagcgat cgctttgcgc gaccggtgcc agcgacatca
     9961 ggtttccggc gtaggtcagc ttatcgacca ctaccaccgc gtcgctcgtt tcgttgatga
    10021 tataacgcac cagcgccgag ccaataaacc cggcaccacc tgttatcaga atttttctca
    10081 tcagcgccag actcctttgg catcgacgac atactgctga tggacattgt cgccattgat
    10141 aactttaaac tgactatgat cgaccagcat caccagcacg tctgccgttg ccagcgcctc
    10201 gtcaagctgc gccagagtac aaagcccggt cagttttttc ggcaactggt ggatgttagg
    10261 ctcaacaacc agagtttcgc cgctatgcca ctgggcgatc agttcagcga tttccatcgc
    10321 cgggctttcg cgcaggtcat caatattcgg tttaaacgcc agaccaaagc aggcgatttt
    10381 cagttcactg gcgcgtttat cggtagccgc caggcaatca gccaccgccg ctttcacctg
    10441 atcgataacc cagaacggtt tgtgatcgtt cacttcgcgc gcggtacgga taagccgcgc
    10501 ctgctggggg ttctgtgcca cgataaacca cggatcaaca gcaatgcagt gaccgcccac
    10561 gccagggcca ggctgaagaa tattaacgcg agggtgacga ttcgccaggc gaatcagttc
    10621 ccagacgtta atcccctgat cggcacaaat cagcgacaat tcattagcaa aagcgatatt
    10681 cacatcgcgg aagctgtttt cggtgagctt acacatttcc gccgtccgcg agttagtgac
    10741 gacacactca ccttcgagga aaattttgta cagttcgctg gcgcgggtcg aacaaaccgg
    10801 cgtcatacca ccaatcacgc gatcgttttt aatcagctcg accataacct ggcccggtaa
    10861 cacgcgttcc gggcagtaag caatgttgac gtccgcctgc tcgcccacct gctgtgggaa
    10921 agtgagatcc ggacgcatct ctgctaacca ttctgccatc ttctcggttg accccaccgg
    10981 cgaggtggat tcaaggatca ccagcgcgcc ttttttcagc actggcgcaa tggagcgagc
    11041 agccgattca acgtaggtca tatctggctc atgatcgcct ttaaacggcg tgggtacagc
    11101 aatcagccag gcatccgctt caactggcgt cgtgctcgct cgtaaaaaac cgccttctac
    11161 ggcagttttt actacgctcg ccaaatcagg ctcgacgata tggatttcgc cacgattgat
    11221 ggtatcaacc gcgtgttggt tgatatcgac accaatcacc tgtttttgcc gtgaggcaaa
    11281 cgctgctgct gtaggcagcc cgatgtaacc cagtccgata acagaaatgg tcgcaaaact
    11341 catagtgata tccgattatt ttttaacgct tccagaatgc gagagcatgc ctgaccatca
    11401 ccatacgggt tatgggcgcg gctcatagtt tgatattcgt tttcgtcttt taaaagacgc
    11461 gtcacttcct cgacaattcg ctgcttatcc gtgcctacca gacgcaccgt acccgccgtc
    11521 accgcttccg gacgctcagt ggtatcgcgc atcaccagca ccggtttccc cagcgaaggc
    11581 gcttcttcct gaatgccgcc tgagtcggtc aaaatcagcc aggcgtggtt catcaaccag
    11641 acaaacggta aatactcctg gggatcgatc agaatgacat ttttcacatg ccccagaatg
    11701 cgattgaccg gctctctgac gttcgggttg agatgcaccg gatagacaat ctggatgtcc
    11761 tggtgcgtgg tggcaatgtc tgccaacgcg tggcagattt cttcaaaacc acgaccgaaa
    11821 ctctcacgcc tgtgaccggt caccagaatc atctttttat tggggtcgat aaacgggtaa
    11881 tttgccacca gttctgaacg cagcgtgtcg ctgctcatca cctgatcacg cacccataac
    11941 agtgcatcaa tgactgtatt accagtaatg aagattcggc tatccgcaac gttttcacgc
    12001 agcaagtttt gccgggaagt ttcggttgga gagaagtgat acatcgccag atgcccggtc
    12061 aatgtacggt tagcctcttc cggccacggc gaatagagat cgcccgtgcg taaaccagcc
    12121 tcaacgtgac caacaggaat acgctgataa aacgccgcca ggctggttgc cagcgtcgtt
    12181 gttgtgtcgc cgtgaaccag cacgacgtct ggtttgaact cggcaagaat aggttttagc
    12241 ccttccagaa tccgacaggt tatctctgtc aggccctgtc ctggctgcat tatgttgaga
    12301 tcgtagtcag gtacaatgga aaagagtttc agcacctgat cgagcatctc ccgatgctgc
    12361 gcagtgacgc aaactttagc ctcaaaaaaa ggagcttttg ccaacgcatg caccaacgga
    12421 gccatcttga tggcttccgg gcgcgtacca aatacagtca gtactttcac atcgattctc
    12481 ttcgaataag cggcgagcgc ctttgcgctc gccgcagcag tgttgctatt tcgagcaacg
    12541 gcgggttaat gcgacaccag ccccgatcag ccccccgaca atgccccaca taatcatcag
    12601 gaaggcacga cgtgggctat cgcgttttac cggttcttcc ggcgtacgca aatagcgata
    12661 ggtctgaaaa cgcggatcca gggttggacc aacattcagg gtgtttaaca tggcccgatt
    12721 ctgatcatag tcgagatcaa aggccggacc gacggcctgt aaattttcca gtcgagcctg
    12781 gagcattgga cgcccaagca ggaacatttc tgaatcaggt aattcctcgg caggcacatc
    12841 tgtcgcactg cgcgaaatat tatgctgctc agcaattttc agcgcctgct caatgctgtt
    12901 catccggcgg tcgtagatgg ctttcgccac ctcttcctga cgcttcacct gagctttcat
    12961 ctggatggtg cgtgccgccc atgcgccttt cagctcatca ttcagatggc tggctgcacg
    13021 ctggctggca aaagcaacat actgacgtaa caggttatta gcgtcaggcg cggtttcggc
    13081 aataagcttc acgctgtcat tgaccgcgcg ggtaaagtct ccggggataa actggatgtt
    13141 gttaatcatt tcatccagca acgccgcatc ggctttgctg ttgcccacca tccgctgttt
    13201 gtaatagtcg gtttgcagcc agaactctct gcgggtatcc cacgaggcca gttgcataac
    13261 aaactcttta taggcttcgt ccatgaccga tggttgatcg gcagaagcca tgtttgaacg
    13321 gacatccagg ttacgcaaaa attgctgctg cgagtaatat ccccccagca tattcaccgt
    13381 tggacgatcg gtaatcgccg tcgagctcca ctcctgacga gcaaaaaaag tatacgccag
    13441 cgcgattaac gcaaacgcca gccccatgcc aataatccat agcttcccag cccacaaggt
    13501 acgaaacaac ccacgaatat ccagttcatt ttcagcgtct tcggccggtt tcccaggcat
    13561 tggttgtgtc atcacatcct catttatttg gttaaattgg ggctgccacc acgatttcta
    13621 cgcagtctgc gttttacgcg cttaataaag cgagcaactt tccaggcacg cttaatgcaa
    13681 tatccataga ggaagaatgc tagcaaaaag agcaccagca tgacccactc cgggacaaaa
    13741 tgagaatatt ctgccagcac gccaatggaa gcgagcagtg ctgcggcaag ggtaatcagc
    13801 acaaacgcct gacgggaagt aaacccggca cgcatgatca aatggtgaat atgctgacgg
    13861 tcaggagaga atgggctcat gcctttacgc aggcgacggt acataatcgc caccatatcc
    13921 attagcggaa tggcgattat ccacaaagcg gtaaccgggc tgatgggatg ggttttgccc
    13981 tgggtcgttt cgagcaggat ccagataacg gtaaaaccaa tcagcgtact gcccgcatca
    14041 cccataaaga ctttgtagcg gcgacccagg ataccaaggt taagcatgat gtatggcagg
    14101 atggcggcga tcatcgcaaa gcaccagatt gcgaggctgg tttgcccgtc gaaccacaaa
    14161 atcataccga ttgctgcaaa cgagacgcag gacaacccgc ccagcaagcc atcaatgcca
    14221 tcaaccatgt tgaacgcatt aatggccgcc cagacggcaa atagcgtcag gaagtaacca
    14281 aacggtccga gcgccatctc ccaggagcca aagatataac ccaggctact gagataaagt
    14341 ttgccgaaca ccatcataac aatgccaaca gcggcctgta tggtggcacg gatttttacg
    14401 ctgatatcaa aacggtcatc cagcgcgcca atgaaaacaa gcacaccggc acaagcgaga
    14461 tagagagatg catgcggaat atagtaatcg acaattccga acgtgaagca aatccctgcg
    14521 taaaccgaaa tccccccaac gagaggtatc aatccctggt gacgtttgcg gaagtttggt
    14581 ttatccacta aaccgacttt ttttgccacc ttacgggcaa aaaacagaaa cagtgtcgtg
    14641 aataaaaaaa tactgatgag atcagtactc actgtcagta aattcacaat gcatgctctc
    14701 agagaaaatt attagcagaa gtataaccac gaagaccttt attcagaagg gaaagtccgt
    14761 tccgaaccca ccagtcctgt gcaaaaatta accatttgat atgcaaattg cataacatgc
    14821 cagcaaattc caggaatttt cctaacatcg ccagcgcggc attaagatta cagatataaa
    14881 agcaaaacgc cacgtaaaca cgtggcgttt ttggcataag acaaatttat gagcgtttca
    14941 tcatttcgaa gaaatcgtca ttggtcttgg tcatggccag tttattaatg aggaattcca
    15001 ttgcatcgat ttcgcccatc gggtgaatga ttttgcgcag gatccacatt ttctgcagtt
    15061 cttcctgagt cgtgagcagc tcttctttac gggtaccgga acggttgtag tcgatagccg
    15121 ggaagacgcg tttttcagcg atcttacgag agaggtgcag ttccatgttg cctgtacctt
    15181 taaactcttc gtaaataact tcgtccattt tagaaccggt atcgataagc gccgtcgcga
    15241 taatggtcag gctgccgccc tcttccacgt tacgcgccgc accgaagaag cgtttcggac
    15301 gatgaagggc attggcgtcc acaccaccgg tcaacacttt acctgacgcc ggaacaacag
    15361 tgttgtaagc gcgcgccaga cgagtgatgg agtcgagcag aatgataacg tctttcttgt
    15421 gctcaaccag gcgtttggcc ttctcgatca ccatttccgc aacctgaacg tggcgagatg
    15481 cgggttcgtc aaaggtagaa gcaacaactt caccttttac cagacgctgc atctcggtta
    15541 cttcttccgg acgttcgtcg atcagcagaa ccatcagcac acaatccggg tggttgtaag
    15601 caatgctctg agcaatgttc tgcagcagca tagttttacc ggctttcggc ggtgccacaa
    15661 tcagaccacg ctgaccacga ccgataggtg atgccagatc cagtacgcga gcggttaaat
    15721 cttccgtaga accgttacca cgttccatac gcagacgaga gtttgcgtgc agcggggtta
    15781 agttctcaaa gaggattttg ttgcgggcgt tttcaggttt gtcgaagtta acttcgttaa
    15841 ctttcagcag cgcaaaatag cgttcacctt ctttcggcgg gcgaatctta ccagagatgg
    15901 tatcaccagt gcggaggttg aaacggcgga tttggctagg ggaaacgtag atgtcatcag
    15961 gaccggcgag gtaggagctg tctgcggaac ggaggaaacc aaatccatcc tgcaatatct
    16021 ccagtacgcc atcaccaaag atatcttcgc cactctttgc gtgctgcttc aggatggcaa
    16081 aaataatgtc ctgcttacgc atacgagcca ggttttccag ccccatattt tcgccgagag
    16141 tgatcagctc agaaaccggc gtattcttta attcggtaag attcatagtg gtgtgagttc
    16201 ttaaacttgg ggtaaatctc gaacttaatg ttgtgaatgg tatggcagga tcatccatgc
    16261 ctgtttacgg tcatcgactc atgtcttttc gctgcctggt cacaggagag tacgcagaac
    16321 tgaaacgaca agacggattg agtgacgagc cagaaatctg ttcacttcgc atttaagata
    16381 aaacgggaag cgttggaaat tacaagattc aaacttaata aggtatgttt aatacgaagt
    16441 caacactaag ttagcatgac tcacgccggg cgtccagttt ttagcgacgg ggcacccgaa
    16501 catgaaattc ccttacgcca ggttagcgtc gaggaactct ttcaactgac ctttagacag
    16561 tgcacccact ttggttgccg ccacttcacc gtttttgaac agcagcagag tcgggatacc
    16621 acggatgcca tatttcggcg cagtgccagg gttttgatcg atgttcagtt ttgcaacggt
    16681 cagtttgccc tgatattcgt cagcgatttc atccagaatc ggggcgatca ttttgcacgg
    16741 accgcaccac tctgcccaga aatcgacgag gatcgccccg tccgctttga gtacatccgt
    16801 gtcaaaactg tcgtcagtca ggtgaataat tttatcgctc atatataact ccacaggaat
    16861 aagcctggcg tgttggtttc gttgttggtg taacattaac caactaaagg ttgactttat
    16921 ttcaccggat acgctttcgt aaagcaatag taagctgata ttctaccaca ctatgagcaa
    16981 aacacattta acagaacaga agttttccga cttcgccctg catccgaagg ttgtagaagt
    17041 ccttgaaaaa aaagggtttc ataactgtac gcccattcag gcactggccc ttccgctgac
    17101 gctggcgggt cgtgacgtag ccgggcaggc gcaaaccggt accgggaaaa cgatggcgtt
    17161 tcttacgtca acgtttcatt atcttctctc tcatcctgcg attgccgatc gcaaggtgaa
    17221 tcaaccacgt gccttaatta tggcaccgac gcgtgaactt gccgtgcaga tccatgccga
    17281 cgcagaaccg ctggcggaag ctaccggcct gaagctgggt ctggcttacg gtggtgatgg
    17341 ctacgacaaa cagctgaaag tgctggaaag cggcgttgac attctgattg gcactacggg
    17401 gcgtttaatt gactacgcca agcagaacca cattaacctc ggtgccattc aggtggtggt
    17461 actggacgaa gccgatcgta tgtacgatct gggctttatt aaagatatcc gctggctgtt
    17521 ccgccgtatg ccgcctgcaa accagcgcct caacatgctg ttctccgcca cgctttcgta
    17581 ccgggtacgt gaactggcgt tcgagcagat gaacaatgcc gaatatattg aagtggaacc
    17641 ggaacagaaa acgggccacc gtattaaaga agagcttttc tacccttcta acgaagaaaa
    17701 aatgcgtttg ctgcaaacgc tgatcgaaga agagtggcca gacagagcga ttattttcgc
    17761 caataccaaa caccgttgtg aagagatctg gggccacctg gcggcagatg gtcatcgtgt
    17821 cggtttgttg acaggcgatg tcgcgcagaa aaaacgtctg cgtattcttg atgaatttac
    17881 ccgtggcgat ctggatattc tggttgccac cgacgttgcc gcgcgtggtt tgcatattcc
    17941 ggcagtgacg cacgtcttta actacgattt acccgatgac tgtgaagatt acgttcaccg
    18001 tattggtcgt acaggtcgcg caggcgcaag cggtcactct atcagcctgg cgtgtgaaga
    18061 gtatgcattg aatttgcctg ctattgagac ctatattggt cactcaattc cggtaagcaa
    18121 atacaatccg gacgcattga tgaccgatct gccaaaaccg ctgcgcctca cgcgcccacg
    18181 tacaggcaat ggtccgcgtc gtactggcgc tccgcgtaat cgtcgtcgtt caggttaaat
    18241 aaaatgtcat actcaaaatc attcaaactg tatcaagatg gcaagagaat gaatcccgat
    18301 gagcatactg tagtaagtga ttcaggtgaa agaatgcagc caacacagag acagattgaa
    18361 ggatgaagag tatgggttcc acctcgtcgc tgtatgcagc cattgatctc ggttcgaata
    18421 gttttcatat gctggttgtg cgcgaggtgg ctggaagcat ccagacgctg acgcgaataa
    18481 aacgcaaagt gcgtctggct gctggcctga atagcgaaaa tgccctgtct aatgaagcaa
    18541 tggagcgcgg ttggcaatgt ctgcgcctgt ttgctgaacg tctgcaagat atccctccct
    18601 cgcaaattcg cgttgtcgct acggcgacat tacgtcttgc cgtcaatgcg ggtgatttta
    18661 ttgccaaagc gcaggaaatc ctcggttgtc cggtacaggt gatcagcggt gaagaggaag
    18721 cacgtctgat ttatcagggc gttgctcaca caactggcgg tgccgatcag cgcctggtgg
    18781 tggatatagg cggtgccagt actgaactgg taaccggcac gggtgcacaa accacctcgt
    18841 tgttcagcct gtcgatgggc tgcgtcacct ggctggaacg ctattttgcc gatcgtaatc
    18901 tggggcagga aaattttgat gctgcagaaa aagcggcacg cgaagtgtta cgtccggttg
    18961 ccgatgaatt acggtatcac ggctggaaag tgtgcgttgg tgcttccggc accgtgcagg
    19021 cgttacagga aatcatgatg gcacagggga tggatgaacg cattaccctg gaaaagttgc
    19081 agcaattgaa acagcgagcc attcattgcg gtcggctgga ggaactggag attgacgggc
    19141 tgacgctgga acgtgcgtta gtgttcccga gtggtctggc gatcctgatc gccattttta
    19201 ccgaactgaa tattcagtgt atgaccctgg cgggcggtgc gctgcgtgaa ggcctggtct
    19261 acggcatgtt gcatctggcc gtcgagcagg atattcgcag ccgtacgctg cgtaatattc
    19321 agcgccgctt tatgatcgat attgatcagg cgcagcgcgt agccaaagtt gcggctaact
    19381 tcttcgatca ggtggaaaag gaatggcatc ttgaagcaat aagccgcgat ttgctcatca
    19441 gcgcctgtca gcttcatgaa atcggcctga gcgttgactt taaacaagcg ccgcaacacg
    19501 ctgcttatct ggtgcgtaat ctggatcttc ccggttttac ccccgcacag aaaaaattgc
    19561 tggcgacgct actgctcaac cagactaatc cggtcgatct ctcatcgctg catcagcaaa
    19621 atgccgtacc gccgcgcgtc gcagaacaac tctgccgttt actccgcctg gccatcattt
    19681 ttgccagccg tcgccgtgac gatctcgtgc cagagatgac attacaggct aaccatgaac
    19741 tgttgacctt gacgcttccg caaggttggc taacccaaca tccgctgggt aaagagatta
    19801 ttgctcagga aagccagtgg cagagctatg tccactggcc gctggaagtg cattaatgag
    19861 taagtgccgg atgcgatgct gacgcatctt ttccggcctt gattatttcc ctcgttttgc
    19921 cgccatcatc gctttcagat tcgccagatg gctttgccct ttctgcatcc gttcttcggc
    19981 gctgaccact ttgcgctcct gttcccaaat cagatcatcc tgcggcagct ccagcaaaaa
    20041 gcggctcggc tccgggcgca ccagctcgcc gtactgacgg cgttctttac acagcgtaaa
    20101 ggtcaattcc ttctgggcgc gggtaatgcc gacataggcc agccgccgct cctcatcgat
    20161 attatcttca tcgatgctgc tctggtgcgg caaaaaccct tcttccatac cgaccatgta
    20221 gacataagga aactccagcc cttttgacgc gtgcagagtc atcagttgca cctgatccag
    20281 ctcttcttca ctctcaccac gctccatcat gtcgcgcaaa gtaaagcgcg tcaccacctg
    20341 ggtgagcgtc atcggctcat ccagttcgct gccttccagc atctccgtca tccagctaaa
    20401 cagttggttg acgttcttca tgcgcatttc ggcggctttc gggctgggcg atgtttcgta
    20461 cagccaggat tcataatcca tgccgtggat cagatcgcgc accgcggcaa tcggctcccg
    20521 ctccgccaga cgctggattt ctgccaacca gtgagtaaag cgggtcaatg cttcataacc
    20581 acgtccgcta agcgtctgac tcaggcccat atcaaagctg gcggtaaaca tgcttttatt
    20641 gcgcgtcatc gcccactcac ccagcttttt cagcgtcgcc gggccaatct ctctcttcgg
    20701 cgtgttaacg atacgcagaa atgcgctgtc atcgtccggg ttagtcagca cgcgcagata
    20761 agccagcaag tccttgattt caggacgaga gaaaaacgac gtaccaccag aaattttgta
    20821 cgggatgcgg ttttgcatca ggaatttttc aaacactcgc gactgatggt taccgcgata
    20881 aagaatagcg taatatttgt actgcgtttt attgacgaag tgatgcgcaa tcagttcacc
    20941 agtgacgcgc tcggcttcat gttcttcgtt attcgcgctt aaaactttaa gctctgtgcc
    21001 gtaacccagt tcggagaaca gacgcttctc aaagacgtgc gggttattgg cgatcaggat
    21061 gttcgccgct ttcaaaatac gtccggaaga gcggtagttc tgctcaagct taatcacctt
    21121 cagcgccgga aaatcctgac tcagcagcac caggttttgt ggacgtgcgc cgcgccagga
    21181 gtagatcgac tggtcgtcat cgcccaccac ggtaaagcgc gcgcggctgc ccaccagcag
    21241 tttcaccagt tcatactggc tggtgttggt gtcctgatac tcatccacca gcagatagcg
    21301 aattttgttc tgccagcgct cacggacttc ttcattgcgt tgcagcagca acgtcggcaa
    21361 taaaatcaga tcatcgaagt cgagaacatt gcacgctttc aggtgtgcat catacagccc
    21421 ataacaatgg gcaaaaatac ggtcccgctc accaatcgca ctggctgccg cctgggacgg
    21481 tgttttgaga tcgttcttcc agttagagat ggtcgaaatc aactgttgca ggagaacttt
    21541 gtcatcttca atcagccctt cggtcaactc tttgagcaac gccagctgat cggtatcgtc
    21601 aaacagcgaa aagttcgcct tcatccccag cgccgcatac tcacgcttga tgatatccag
    21661 ccccagcgtg tggaaggttg agatcatcag cccacgcgcc tctttgcgac ccagcgtctg
    21721 tccgacacgc tctttcatct cgcgcgccgc cttattggta aaggtcaccg ccgcgatatg
    21781 tcgcgcctga taaccgcaac cgcggatcag atgggcgatt ttattggtga tcacacgagt
    21841 tttaccggaa cccgcgcccg ccagcaccag gcaggggccg gtaacgaatt cgacagcttg
    21901 ttgttggccg gggtttagac gcataggtgt attgctcaat cttcgaacgg ggggaggatt
    21961 gtagcagaat cgcatttgaa tgtgaccgcg tgtaagccgc taaccggttg gctgcggatg
    22021 tttaaatcag agagggaaga ccgaaaccgg agttcatcgt gaatccatta gacttagaaa
    22081 atatcgggtc gtaacgttgt gttattactt tttcagagtt tactaaggag aggtgtactc
    22141 gtggcgatta atttcagtcc taaggttggt gaaatactgg aatgcaactt tggtaactat
    22201 cctgttagcc agaatggaca gttcagcacc acgtattacg atggccgcat tcctcctgaa
    22261 atgataaaaa acaggtta
//
LOCUS       DADLFA010000057        20734 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000057 DADLFA010000000
VERSION     DADLFA010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20734
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.057"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..260)
                     /locus_tag="JQH65_001808"
     CDS             complement(<1..260)
                     /locus_tag="JQH65_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6139247.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAILRCTGNSGHYH"
     gene            complement(513..983)
                     /gene="bcp"
                     /locus_tag="JQH65_001809"
     CDS             complement(513..983)
                     /gene="bcp"
                     /locus_tag="JQH65_001809"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HAY6139248.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(983..1555)
                     /locus_tag="JQH65_001810"
     CDS             complement(983..1555)
                     /locus_tag="JQH65_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HAY6139249.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            1701..2579
                     /gene="dapA"
                     /locus_tag="JQH65_001811"
     CDS             1701..2579
                     /gene="dapA"
                     /locus_tag="JQH65_001811"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAY6139250.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            2596..3630
                     /gene="bamC"
                     /locus_tag="JQH65_001812"
     CDS             2596..3630
                     /gene="bamC"
                     /locus_tag="JQH65_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HAY6139251.1"
                     /translation="MAYSVQKSRLAKVAGVSLILLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGVRTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTIDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSASDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            3843..4556
                     /gene="purC"
                     /locus_tag="JQH65_001813"
     CDS             3843..4556
                     /gene="purC"
                     /locus_tag="JQH65_001813"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAY6139252.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKRFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            4724..5587
                     /locus_tag="JQH65_001814"
     CDS             4724..5587
                     /locus_tag="JQH65_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HAY6139253.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHNMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            5602..7617
                     /locus_tag="JQH65_001815"
     CDS             5602..7617
                     /locus_tag="JQH65_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Met) cytidine acetyltransferase"
                     /protein_id="HAY6139254.1"
                     /translation="MAELTALYTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALL
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQYFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGVGQQLIASALQYTQDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYCLRRDAQALAQWNGETLPVD
                     PLNDIVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLAGRKMLLVRQREEAAHALFALNDVRTERLRDRITQWQFFH"
     gene            7691..8389
                     /gene="ypfH"
                     /locus_tag="JQH65_001816"
     CDS             7691..8389
                     /gene="ypfH"
                     /locus_tag="JQH65_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAY6139255.1"
                     /translation="MKHDHFVVQSPDKPVQQLLLLFLGVGDNSVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(8499..8699)
                     /locus_tag="JQH65_001817"
     CDS             complement(8499..8699)
                     /locus_tag="JQH65_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HAY6139256.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(8727..9854)
                     /gene="dapE"
                     /locus_tag="JQH65_001818"
     CDS             complement(8727..9854)
                     /gene="dapE"
                     /locus_tag="JQH65_001818"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HAY6139257.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(9858..10214)
                     /locus_tag="JQH65_001819"
     CDS             complement(9858..10214)
                     /locus_tag="JQH65_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HAY6139258.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPSIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            10595..10654
                     /gene="ypfM"
                     /locus_tag="JQH65_001820"
     CDS             10595..10654
                     /gene="ypfM"
                     /locus_tag="JQH65_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HAY6139259.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(10753..13865)
                     /gene="acrD"
                     /locus_tag="JQH65_001821"
                     /pseudo
     CDS             complement(10753..13865)
                     /gene="acrD"
                     /locus_tag="JQH65_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
     gene            complement(14028..15728)
                     /gene="narQ"
                     /locus_tag="JQH65_001822"
     CDS             complement(14028..15728)
                     /gene="narQ"
                     /locus_tag="JQH65_001822"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HAY6139260.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQHLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            15934..17913
                     /gene="aegA"
                     /locus_tag="JQH65_001823"
     CDS             15934..17913
                     /gene="aegA"
                     /locus_tag="JQH65_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HAY6139261.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     LAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            17981..18556
                     /gene="nudK"
                     /locus_tag="JQH65_001824"
     CDS             17981..18556
                     /gene="nudK"
                     /locus_tag="JQH65_001824"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HAY6139262.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            18682..19725
                     /locus_tag="JQH65_001825"
     CDS             18682..19725
                     /locus_tag="JQH65_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HAY6139263.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPEASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKNPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(19835..20730)
                     /locus_tag="JQH65_001826"
                     /pseudo
     CDS             complement(19835..20730)
                     /locus_tag="JQH65_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300650.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
BASE COUNT         5010 a         5301 c         5549 g         4874 t
ORIGIN      
        1 aaatggtagt gtccgctatt gccagtacac ctcaggatcg catcatccct ctgggagatg
       61 gcattgaccg ggcagacctg caggcaaggg gcttcctcac agtgatgaca cacgacaggg
      121 gcagtgattg ttgacgtctt cgccagggcc aggcgcgggt gttgctgtaa accttgcgtt
      181 ttatgcacgt ccgaacaggc agcgagacag gtattacatc ctgtacacca cagtggttcg
      241 gccaccacaa agcggttcat gtcggctcct tgaaaatgat atgaaacatt gcatttcata
      301 ttgcataaag gatgccagtc tgattaattt aatatattca ataaattatg tattaacagg
      361 gttctgaacg gtacaaaaat gatggtcatt aacgttcagc aaaaaatgga ttcgacaccc
      421 gtgtcgcagg ggaaagcgag aatgtgatct tgatcacgag aagtgaggtg tgctgaccgc
      481 aagcgcagcc agcacggaat ggagcaaagt aatcaggcgt gttctttcag ccagttcagc
      541 acaacgtcgt ggtgattgct ggttttaaaa tcgtcaaaga catgttcgat tttgccatca
      601 gcgtcaatca ggaagctgat gcgatgaata ccatcgtagg ttttgcccat gaaggacttt
      661 tcaccccaga cgccgaattg ttcgcacacc tggtggtcct catcagacag gagcgtaaag
      721 ttaagcagct ctttttccgc aaaacgggag agtttttcgg gtttatcggt gctgataccc
      781 agcacatcaa cgcccgcttt tttcaactca tccatgttat cgcgtaagcc gcaggcctgt
      841 acggtacagc cgggggtcat ggctttcggg tagaaataaa ccagaacacg ctgtccctgg
      901 aagtcggtca aattaacttg ttctccgtct tgatccggca agctaaattt cggtgcgata
      961 tcaccggctt tcagtggatt cattacttaa ctccatcctg ttcatcatgt tgggaataat
     1021 tgacgacgtt aatactgcct tgtgcattga gttctgtaca tagggctttg aacgcttgct
     1081 caatatttgc tgcgtccgca gatgcggggc tgtgggcggt gatctgaata tgcaactgcg
     1141 cagcccgttc attttcagca ggttgcgtgc gcgacaccag ctccgcaatg ttcatatgat
     1201 gcgcgtcgaa aagtgctgtg aagcgttcaa ttaaatgcgg ggagtctgca acatcgacct
     1261 gaacccagac agatgctggc attggcggac gcggacgcgc cgtcgtgcgc ttcatcacga
     1321 ttaaaagatc cagttcggca cctttcaacg gtaacgttga ttcaatcaga gtaatggcat
     1381 tccatgaacc ggaaagcagc atgataaacg tgaactcttc tcccagcatc gccaggcgac
     1441 tgtcttcaat attacagccg caactactga catgacgggt gatggtgttc acaattccag
     1501 ggcgatcggc acccaacgca gtgatcacca gataatgttg cgatgacagt gtcaaactgg
     1561 ttattccttt aaggggtgag ttgttcttaa ggaaagcata aaaaaaacat gcatacaaca
     1621 atcagaacgg ttctgtctgc ttgcttttaa tgccatacca aacgtaccat tgagacactt
     1681 gtttgcacag aggatggccc atgttcacgg gaagtattgt cgcgattgtt actccgatgg
     1741 atgaaaaagg taatgtctgt cgggctagct tgaaaaaact gattgattat catgtcgcca
     1801 gcggtacttc ggcgatcgtt tctgttggca ccactggcga gtccgctacc ttaaatcatg
     1861 acgaacatgc tgatgtggtg atgatgacgc tggagctggc tgacgggcgc attccggtga
     1921 ttgccgggac cggtgctaac gctactgcgg aagccattag cctgacgcag cgcttcaatg
     1981 acagcggtat cgtcggctgc ctgacggtaa ccccttacta caatcgtccg tcgcaagaag
     2041 gtttgtatca gcatttcaaa gccatcgctg aacatactga cctgccgcaa attctgtata
     2101 atgtgccgtc ccgtactggc tgcgatctgc tcccggaaac ggtgggccgt ctggcgaaag
     2161 taaaaaatat tatcggaatc aaagaggcaa cagggaactt aacgcgtgta aaccagatca
     2221 aagagctggt ttcagatgat tttgttctgc tgagcggcga tgatgcgagc gcgctggact
     2281 tcatgcaatt aggcggtcat ggggttattt ccgttacggc taacgtcgca gcgcgtgata
     2341 tggcccagat gtgcaaactg gcagcagaag ggcattttgc cgaggcacgc gttattaatc
     2401 agcgtctgat gccattacac aacaaactat ttgtcgaacc caatccaatc ccggtgaaat
     2461 gggcatgtaa ggaactgggt cttgtggcga ccgatacgct gcgcctgcca atgacaccaa
     2521 tcaccgacag tggtcgtgag acggtcagag cggcgcttaa gcatgccggt ttgctgtaaa
     2581 gtttagggag atttgatggc ttactctgtt caaaagtcgc gcctggcaaa ggttgcgggt
     2641 gtttcgctta ttttattact cgctgcctgt agttctgact cacgctataa gcgtcaggtc
     2701 agtggtgatg aagcctacct ggaagcggca ccgcttgcgg agcttcatgc cccggctgga
     2761 atgattttgc cggtgacctc cggtgattat gcaatcccgg tgaccaacgg tagtggtgct
     2821 gtcggtaagg cgctggacat tcgtccacca gcccagccgc tggcactggt ttctggcgtg
     2881 cgtacccagt tcacgggcga taccgcttca ttgctggtgg aaaatggtcg tggcaatact
     2941 ctgtggccgc aggtggttag cgtgctgcag gcgaaaaact acaccatcac ccaacgtgat
     3001 gatgctggtc agacactgac catcgattgg gtacaatgga accgtctgga cgaagacgag
     3061 cagtatcgtg gtcgttatca aatctctgtt aagccgcagg gttatcagca ggcggttacg
     3121 gttaaactgc tgaacctgga acaggcgggc aaaccggttg cagacgcggc ttccatgcag
     3181 cgttacagca cggagatgat gaacgttatt tccgccggtc tggataaatc tgcctctgac
     3241 gccgcgaacg ctgcgcaaaa tcgtgcctcc accactatgg acgtacaaag tgcagctgat
     3301 gacaccggtt taccgatgct ggtcgtacgc gggccgttca atgtggtttg gcaacgtctg
     3361 ccagcggcgc tggaaaaagt gggcatgaaa gtgaccgaca gcacccgttc gcagggtaac
     3421 atggccgtaa cttataagcc gctgtctgac agcgactggc aggaactggg cgcgagcgat
     3481 ccaggcctgg catccggtga ctataaactg caggttggcg atttagataa ccgcagcagc
     3541 ctacagttca tcgatccgaa aggtcatact ctgactcaga gtcagaacga cgcgctggta
     3601 gctgtcttcc aggctgcgtt tagcaagtaa aaatacaggg ctggagtcat ccggcccttt
     3661 tttctgatat gatacgcaaa cgtgtgcgtc ggcagaaaaa tgcaatttta gcggtaattc
     3721 gcacgaaatt tgtttgtcgg acgtaggtcg gataaggcgt tcttgccgca tccgacaaaa
     3781 catccggcac accagacagc aaaagatttt aaaacgttaa ttcacaccca ggagtgataa
     3841 agatgcaaaa gcaagctgag ttgtatcgtg gtaaagcgaa aaccgtatac agcacggaaa
     3901 acccggacct gttggtgctc gaattccgca atgatacgtc agcaggggat ggcgcgcgca
     3961 ttgagcagtt tgatcgcaaa ggtatggtga acaacaagtt caactacttc attatgagca
     4021 aactggctga agcgggtatc ccgactcaaa tggagcgtct gctctccgat accgaatgtc
     4081 tggtgaaaaa gctggatatg gtgccggttg agtgtgtcgt gcgtaaccgt gctgctggct
     4141 ctctggtgaa acgtcttgga atcgaagaag gtattgagct gaacccgccg ctgttcgatc
     4201 tgttcctgaa aaacgatgcc atgcacgatc cgatggtcaa cgaatcttac tgcgaaacct
     4261 ttggctgggt gagcaaagag aacctggcgc gtatgaaaga gctgacctac aaagcgaacg
     4321 acgtgctgaa aaaacggttc gatgatgctg gtctgattct ggtcgacttc aagctggagt
     4381 ttggtctgta caaaggtgaa gtggtactgg gcgatgagtt ctccccggac ggcagccgcc
     4441 tgtgggacaa agaaacactg gagaaaatgg acaaagaccg tttccgccag agcctcggtg
     4501 gcctgatcga agcctatgaa gccgtcgccc gccgcctggg tgtacagctg gactgatttt
     4561 tctgttcatc atcttgccgt gcagctggca cggcaagaca accgctctcg gaatatatgc
     4621 attgaacgct cattcctccg ccatttcatc ccgctttcct gtggtaatct tcctctgaac
     4681 cacctacgat catttctata atgaatatat tgttgaggta tctatgcgtt ggcaagggcg
     4741 acgtgaaagt gacaatgttg aagacaggcg caacagctcc ggtggtccgt ctatgggcgg
     4801 tcccggtttt cgcctgccaa gcggtaaagg cgggctgatt ttactgatag tcgtgctggt
     4861 tgcaggctac tatggtgttg atttaaccgg gttaatgact ggacagccgg tttcccaaca
     4921 acaatcaacg cggtccatta gccctaatga agacgaagct gcaaaattca cctcggtgat
     4981 tctggcaacc acggaagaca cctggggaca acagttcgag aagatgggta agacctatca
     5041 gcaaccgaag ctggtcatgt accgtggaat gacgcgcacc ggctgcgggg cgggccagtc
     5101 cattatgggg ccgttctatt gcccggcgga tggcacagtt tatatcgatc tctccttcta
     5161 tgatgacatg aaagacaaac ttggcgcgga tggcgatttt gcccaggggt acgttatcgc
     5221 ccatgaagtc ggtcatcatg tgcagaaact gttaggcatc gagccgaaag ttcgtcaact
     5281 gcaacaaaac gcgacgcagg cggaagtgaa ccgcttatct gtgcgtatgg aactccaggc
     5341 cgactgtttt gccggtgtct gggggcataa catgcagcag caaggcgttc tggaaaccgg
     5401 cgatctggaa gaggcgctga atgcggcgca ggccatcggc gatgaccgtt tacaacagca
     5461 aagtcagggg cgagtagtac cagacagttt cacacatggc acttctcagc aacgctacag
     5521 ctggtttaaa cgtggtttcg acagcggcga tccggcacaa tgcaatactt ttggtaaaag
     5581 catttaattt ccggggcagg gatggctgaa ctgactgcgc tttacacatt aacagcgcaa
     5641 atgaaacgtg aagggatccg ccgcttgctg gtgttgagcg gggaagaggg ttggtgtttt
     5701 gatcatgcgc ttaagttgcg tgatgcctta cttggcgact ggctgtggat ttcgccgcag
     5761 ccagatgctg aaaaccactg ttctccctcg gcgctacaaa ctttacttgg gcgcgagttc
     5821 cggcatgcgg tattcgacgc ccgccacggc tttgatgccg ctgcctttgc ggcacttagc
     5881 ggaacgttga aagcgggaag ctggctggtt ttgttactcc ctgtatggga agagtgggaa
     5941 aaccaacctg atgccgactc gctgcgctgg agtgattgcc ctgaccctat tgcgacgccg
     6001 cattttgtcc agcatctcaa acgcgtactt acggcggata acgacgctat tctctggcgg
     6061 caaaaccagc cgttctcgtt ggcgcatttt actccccgta ctgactggca ccccgcgacc
     6121 ggcgcaccac aaccagaaca acagcaactc ttacagcagc tactgaccat gccgccgggc
     6181 gtggcagcgg taacggctgc gcgtgggcgc ggtaagtcgg cgctggcagg gcaactcatt
     6241 tctcgtattg cgggcagtgc gattgtcacc gcgcccgcaa aagcggcaac ggatgtactg
     6301 gcacaatttg cgggcgagaa gtttcgcttt attgcgccgg atgccttgtt agccagcgat
     6361 gagcaagccg actggctggt ggtcgatgaa gccgcagcca tacctgcgcc gttgttgcat
     6421 caactggtat cgcgttttcc tcgaacgttg ttaaccacta cggtgcaggg ctacgaaggc
     6481 accggacgtg gttttttgct gaaattttgc gctcgctttc cgcatttaca ccgttttgaa
     6541 ctgcaacagc cgatccgctg ggcacaggga tgcccgctgg aaaaaatggt tagtgaggca
     6601 ctggtttttg acgatgaaaa cttcacccat acaccacaag gcaatatcgt catttccgca
     6661 tttgaacaaa cgttatggcg aagcgagcca gaaacgccgt taaaggttta tcagttattg
     6721 tctggtgcgc actaccggac ttcgccgctg gatttacgcc ggatgatgga tgcaccaggg
     6781 caatattttt tacaggcggc tggcgaaaac gagattgccg gagcgctgtg gctggtggat
     6841 gaggggggat tatctcaaca actcagtcag gcggtatggg caggttttcg tcgcccgcgg
     6901 ggtaatctgg tggcccagtc gctggcggcg cacggtagca atccactggc agcgacattg
     6961 cgtggacggc gggtcagccg gatagcagtt catccggctc gtcagcgcga aggcgttggg
     7021 caacagctca ttgccagcgc tttgcaatat acgcaagacc tcgactatct ttcggtgagt
     7081 tttggttaca ccggggagtt atggcgtttc tggcaacgct gcggttttgt gctggtacga
     7141 atgggtaatc atcgtgaagc cagcagtggt tgctatacgg cgatggcgct gttaccgatg
     7201 agtgatgcgg gtaaacagct ggctgaacgt gagcattact gtttacgtcg cgatgcgcaa
     7261 gctctcgcgc agtggaatgg cgaaacactt cctgttgatc cactaaacga tatcgtgctt
     7321 tctgacgacg actggcttga actggccggt tttgcttttg ctcatcgtcc gctattaacg
     7381 tcgttaggtt gcttattgcg tctgctacaa accagtgaac tggcattacc ggcgctgcgt
     7441 gggcgtttac agaaaaatgc cagtgatgcg cagttatgta ccacacttaa acttgcaggc
     7501 cgcaagatgt tactggtccg tcagcgggaa gaggccgcgc atgcgctgtt cgcacttaat
     7561 gatgttcgca ctgagcgtct gcgcgatcgc ataacgcaat ggcaattttt tcactgactc
     7621 cttcaatttt ctcccatggt cattgtgagc cagcggcgta aaattaatga catcagttaa
     7681 ggagttcgcc atgaaacatg accattttgt tgttcaaagc ccggataaac ctgtgcaaca
     7741 gttgttactg cttttcctcg gtgtcgggga taactcggtg gcaatggggg aaataggcag
     7801 ttggtttgca ccgctgtttc ctgatgcgct ggtggtgagt gtcggtggcg cggagccgag
     7861 cggtaatccg gcggggcgtc agtggttttc ggtgcagggt attacggaag ataatcgcca
     7921 ggcgcgtgtg gatgccatca tgccgacgtt tattgaaacg gtacgctact ggcagaaaca
     7981 gagtggagta ggggctaacg ccacggcgtt aatcggtttc tctcaaggcg cgattatggc
     8041 gctggagagt attaaagccg aaccgggcct tgcttcacgc gtcattgctt ttaacggacg
     8101 ctatgcgagc ctgccggaaa ccgcgtctac agcaacgaca attcacctga ttcatggcgg
     8161 tgaagatccg gttatcgatc tggcgcatgc ggttgcggcg caggaagcgt taatcagtgc
     8221 cggtggtgac gtgacgctgg atatcgtgga ggatttaggc catgcgatcg acaatcgcag
     8281 tatgcaattt gccctggatc acttgcgtta taccattccg aaacattatt tcgacgaagc
     8341 gctaagtggc ggtaagcctg gcgatgatga tgtgattgaa atgatgtaaa agacagatta
     8401 cctggcctga taagacgcgt gagcgtcgca tcaggcatcg ttcaccattg ccggatgcgg
     8461 cgttaacgcc ttatccggcc tacggttgag cactcagctt acttcttcgg ttgatccttt
     8521 ttcggccagt cgtcgtcatc gtcccactta tcgttgaaat cacgatgcgg gggaagctcc
     8581 ggcttgttgg cgagaaattt cttgtggtcg acgcgcttga gatctttaat cacgttcagc
     8641 aggacgccta ccaaaaagac aatcaccaga atccaccaat atttagccag ccagtccatg
     8701 cttatttcct cttgcagaac cactcatcag gcgacgagct gttccatgat acgttgatac
     8761 atacgggcaa gtagctgcag gtcggcagcg ttcacacatt cattaatttt atgaatagtg
     8821 gcattgaccg gcccgagttc caccacctgc gcccccatgc gagcaataaa gcgcccgtcg
     8881 gacgttccgc ctgtggtcag tagctgcggt ttaatttcat tatagtgctc aaccgcgtta
     8941 acgaccgcat ccaccagttt accgcgcgcg gtcaaaaatg gctgcccgga aagccaccaa
     9001 tccaccgtat agcgcagttg atgtttttca agcagggcaa gcacctgcgc tttgatcatc
     9061 tcatcagtca gttcggtgct gaagcggaag ttaaactgca caaacagttc acccggaata
     9121 acattgttac tgcccgttcc cgcctgaata ttagcaatct gcatactggt cgccgggaaa
     9181 aattcattgc cctgatccca ctcaatagcc actaattcat taaggaaagg tgctgcgcga
     9241 tgtactggat tgtcagccag atgcgggtag gccacatgcc cctgaacgcc atgaatggta
     9301 aggttgcagg ttaacgatcc gcgacgacca tttttcacca catcaccaac cacttcgata
     9361 ctcgacggtt cgccgaccag gcagtaatcg agacgctcat tacgtgccat caacgcttcg
     9421 acgactttta ccgtaccgtt gtgggcactg gcttcttcat cagaggtgat cagaaatgcc
     9481 agtcgccccg tatggttggg atgttgtgcg acaaagcgtt ctgccgctac caccatcgcc
     9541 gccagcgagc ctttcatatc tgccgcaccg cgcccgaata acatgccatc acgaatggtg
     9601 ggttcaaacg gcggattgat ccaacgatcg gcgtcgccag gcggcaccac gtcggtatgc
     9661 ccggcaaagg ctaacgtttc accttgccca cgccatgccc aaaaattctg cgtatcggca
     9721 aagtccatgc gttcaacggt aaaaccgatc gcctgcaaac gttcaatcaa caaagcctgg
     9781 catcctgcgt catcaggact cagggaaggg cggcgaataa gctgttgtgt cagctcaata
     9841 accgggcacg acatagacta cacctcatgg aaaaattgct gataactgga atcactgaaa
     9901 cccagcagca taggcttacc aggcgcgcag agcaatggac gtttgataat tgaaggcatt
     9961 tcagtcatta atgccgccgc agaggccgca tcggtgattt tattgcgggt ggtttcgtcc
    10021 agtttacgcc aggttgtacc acgggtgttc agtaacgctt cccagcctaa ttcgttgata
    10081 aaaccgttca ataattcgct gtccagccca tcgacgcggt aatcatgaaa acgatagtcg
    10141 atgttattgg cttccagcca acggcgagcc tttttaatgg tgtcacaatt tttgatgccg
    10201 taaagtgtaa ccataatgaa tcctttttcg atctatgaga ataaatactc atttaagggt
    10261 gaaaacattt tgcctgcaaa tgattatcag gaaatataac attatcgaag tattcaaatt
    10321 aacaactatt aaataatatt aaacattttt aacgtaaaca ttaagaatat tcatcttgtt
    10381 atctgtctgt attttaacca gaattaaatc agtattagat gccgtatgaa tatctcgaaa
    10441 tgttgctgtg tatcacatac aaattatcac cgcaggacca tagtatttac ataaggtttg
    10501 tcccccggag gtagtcatcg cagcaatcga gtaaattagc ttcaccacgg tatattgttt
    10561 ccgtagaata cccataatta taagagaggt tgttatgatt gaacgtgaac tggggaactg
    10621 gaaagacttt atcgaagtta tgcttcgtaa gtaattcctg agacaaagta caaacagcaa
    10681 gaacccgctg agcaggttct taatcggaaa gttgaaaaag gcgacacatt agcatgtcgc
    10741 cttttttatt gcttattccg ggcgcggctt cagcgggaag cggcggcgca ccagtacaaa
    10801 gaacagcggc acgaagtaaa tagccagaat agtggccgaa atcatcccgc ccattacgcc
    10861 agtacccacc gcatgctgac caccggaacc ggcacccgtg ctggttgcca ttggcaatac
    10921 gccgaagata aatgccagcg aggtcatcag aatcgggcgt aaacgctgac ggcaggcgtg
    10981 gagcgtcgct tcaaacaggt cgtggccttt ttggttcatc tcgttagcaa actcgacgat
    11041 caggatggcg tttttcgccg ataaaccaat gaccgttaac aggcccacct ggaagtaaac
    11101 gtcgttttcc agcccgcgca tccaggttgc cagcagcgcg ccgataaccc ccagcggcac
    11161 gaccagcatt accgagaacg gcaccgacca gctttcatac aacgccgcca gacacaggaa
    11221 taccaccagc aaggaaatgg cgtacagcgc cggagcctgc gcgccggaaa gccgctcctg
    11281 atacgacatc gccgtccact ccagaccaaa gccgtttggc aactgcttca ctaacgattc
    11341 cattatatcc atcgccgtac cggtactgac ccccggtgcg gcttccccaa caatctcaac
    11401 cgcagaataa ccgttatagc gttccagacg cggcgagcca gtctcccagc gtgaggtcgc
    11461 gaaagcagag aagggcacca tgccgccatc tttatttcgg acataccaga gattaatgtc
    11521 atctggcagc atgcgatacg gcgcagctgc ctgcacatag actttcttca cacgaccgcg
    11581 atccataaag tcattcacat agctcgaacc ccaggcggtt tgcagtgtgt cgttaatatc
    11641 gtcgatagca acgcccagcg cctgagcttt acgctggtcg atatcaatct gcaactgcgg
    11701 actgtcatcg aggccgttat ggcgcacacg ggttagctcc gggttttccg ccgccagcgc
    11761 cagcaactgg ttacgtgctg ccatcagcgc atcgtgacct gctccagcgt ggtcctgcaa
    11821 ctccatatca aaacccgcag aactaccaag accgctaatt gctggcgggc tgctggcgat
    11881 aacgcgagct tctttaatct tgttaaacgc ctttgttgca cgctcgataa tggcaaacga
    11941 ggtgccggtc ttactgtcgc gttcgctcca gtctttcagg cggataaaca ttcgcgccac
    12001 gttttgcccg ttacccccag ggccagaacc aacggtggca aacaccgaca tgatgttgtc
    12061 tttttcatgg gtgaagtaat atttctcgat ttgctcaacg actttcaggg tctgttgttg
    12121 ggttgaaccg ctgggcaatt gtaccgaggt agtaaacatg ccacggtctt ccagcggtaa
    12181 gaacgacgtc ggcaaacgca ggaacaggaa caccatgccg ccaagcagca ggacataaat
    12241 cacaatccag cgcaggctgc ggtggagaat tttcgccacc cctttttcgt agcgttcggc
    12301 gttgcggtta aacatctggt taaaccaggc aaaaaagcct ttttgcccgt gatgctcacc
    12361 ttttttcagt ggcttaagta gtgtggcaca cagagccgga gtgaggatca tcgctaccag
    12421 tactgacagc accatcgccg caacaatggt aatagagaac tggcgataga tggcaccggt
    12481 ggtgccgccg aagaaggcca ttggtacaaa taccgccgaa agcaccatcg caatcccgac
    12541 cagtgccccc tggatctgcc ccatcgattt acgtgtggct tcgcgaggag tgagtccttc
    12601 ctcactcata atacgttcga cgttttccac caccacgatt gcgtcatcca ccagcagacc
    12661 gatcgccagt accatcgcga acatggttaa ggtgttgacg ctgtaaccga aggcgtaaag
    12721 tacggagaag gttcccatca acaccaccgg cacggcgata gtgggtatca gcgtggcgcg
    12781 gaagttttgc aggaacagat acataacgag gaaaactagg gcgatagctt ccagcagcgt
    12841 tttcaccacg tcttcaatgg aggctttaac aaacgaggtt gtttcatacg ccaccttgta
    12901 ttccagtccg tgcgggaagt actgcgccag ctcgtcgaga cgattgagca ccagctccct
    12961 gtcgccattt cgtttgcgcc ggaggccagt tttaccccca gcccggaggc tggcttacca
    13021 ttgaagcggc taagataatc gtatttctcc gcccccattt cgacggtggc gacatcgccc
    13081 agccttacct ctgagccgtc ttgattcacc cgcaaggtga tatcgcggaa ctgttctggt
    13141 gtttgcagca gtgactgggc gttaatggtg gcgttgagcg cctgcttatc gacggaaggt
    13201 gtaccaccaa gttgcccaac cgcaatctgc gcgttctgtg actcaatggc atcagtgaca
    13261 tctttagccg tcatctggaa actgttgagt ttcgccgggg ccagccagat acgcatggaa
    13321 tattgcgaac cataggcatc gatatccccg acgccattca cgcggcttaa cgggtcctga
    13381 atattactgg caacataatc ggcaatatcc tgtttatcca tcgaaccatc ggtagagacg
    13441 aaagcaatgg tcagaatatt ggtatcgccg gttttacgca ccgtcacgcc ctgattttgc
    13501 accgcctgcg gtaactttcg catggctgat tgcagctggt tttgtacttg ctgcacggct
    13561 tcgtccggat cggtgcctgc tttaaaactt aaagtgacag atgcctgacc ggtgccactg
    13621 ctctgagatg acatatacat gagattatcg aggccggtca tattttgctc gataacctgg
    13681 ggcacggtgt tttccagcgt ctgggccgat gcgccgggat agttagcggt cactcgcaca
    13741 ttcggtggcg cgagatcggg gtattgttca acgggcaatg aaaaaatcgc tagggtccct
    13801 gtcagacaca acaggattgc cagcacccag gcaaaaatgg ggcgatcaat aaagaaattc
    13861 gccattaaaa gaggacctcg tgtttctgcg tatcgttgta ggcttgactt gcttcactgt
    13921 agcggcaagg tacgagccaa tcgtggagaa aaaaggagtt aatgtaaatt atcatgccct
    13981 tttatactga accttaagtg caagtattct ttggtcagta ggaggcatta catcaactga
    14041 ctttcctcac cctctgcaga gcgaaagcta atactcacta acgtgccgcc gccggaaggt
    14101 tgtgaaaaag tcagcgtccc acccagccgt tccgcgcgtt cgcgcatgat attcagacca
    14161 taatgacctt cgggttcttt cggttcaccg ataccaatcc cgttatcacg gatataaacc
    14221 gtgtgattgc cgtccggcgc ggtgacgcaa ctgacggcga tttcgctggc gttggcgtgc
    14281 ttcatcgcat tcagcaccgc ttcgcgaata atttgcaaca aatgcacctg catttgcgca
    14341 tccagcgcaa gggttggcag acggcagtcg agggtcagtt tggcgctggt ttgattttgt
    14401 aacgtatcca gcatttccct caacgcggag gggagatccg cctgctgcag cgtcaggcgg
    14461 aaagtagtca acagctcgcg taactgccga taagcatcat tcaacgcctg ggaaaaatcg
    14521 gccatgatac tttgtgcggt ggcgttatct tccggtatcg aacgcttcag taacgtcaac
    14581 tggatacgta agtaagaaag tacctgagcc agcgagtcgt gcaattcgcg ggcgatggtt
    14641 gcacgttctt ccatcaacaa taaatgctga aaatgcttct gcgcctgatt aaagtacaaa
    14701 ccgcgtccca gcatcgacga aacgctgtta agcagcggtt ctgatgatga aacgtgacta
    14761 ttttgccagt gcagttcgcc gtaaaccgtc tcttgcattg tcaccggtaa aatctgcatc
    14821 ggcaattccg ggtttggttg cccttcgcta atccgccagt tttcaccgac atttaactcc
    14881 agatattcag ccgcttcatt gtcgcgaaca atctgcaaaa tatggcggaa acaatgcaca
    14941 tcaatctggc ttgtgttcag cgcctgcgaa cattgataca acacctccag acgacgcttg
    15001 gcctcgtgga gatcgcgggt cttttcttct actgacgcct ccagcgaaag gtacaattta
    15061 tgcagctcgc tcgacatctg gttaaaggtt tttgcaagca gaccaagctc attcggcaga
    15121 ctggtatcca gcggcggcga gtcgaactgc ccgtgttcaa tacgttgact ggcggtaacc
    15181 agctgattca gcggggcaac cacctgatgg cgtatacggc gcaaagtaaa aaagaccagc
    15241 gtgaaaatac cgataccgcc agccagggaa atcgccacca ccagcagcat tttgcgttca
    15301 gcgtagtgct gtaaagccag cacgaacagg tctatctgat taacataatt attaatattg
    15361 gcctgatacc acggcaaatc gcccttgctg agccgattat tcatctccag ccagttggca
    15421 ttcagatgcg catagcgagt ttttactgct tctggcacat accagacgtt gagattggtt
    15481 aataccggtg aatgcagtgc ctgctgaaac aactggcgat gtgcattgag ttgtggactg
    15541 ccactttgca agtcgtagcc caggcggtaa ctctgcatac gcagcgatcc ggcaatattg
    15601 atagcctcag cgtcgcgcaa actgctcgcc agagttagca gagcgatacc cgtggaaaga
    15661 atcgacagca gcacaatgta aaaaaaggcc cgggccagac tggccgagac gggtcgttta
    15721 acaatcacac gcgtcttctc cacaaaaaat cattaatgtt ccagttcgct gaaacgcaat
    15781 ttagtcagtg atgggattat ttgagctgcc acagtttata gcgaaatagt tacttattct
    15841 gggcacatga acattgccga ttcccctgta agtcgggtaa taacaacact catataaaga
    15901 ataaggtttt tacaaccaaa aaagaaggtc gttatgaatc gttttattat ggccaacagt
    15961 cagcaatgtc tgggttgtca tgcttgtgaa atcgcctgtg tcatggctca caatgatgag
    16021 caacatgtcc tgagccaaca ccattttcat ccccgaatta cggttatcaa acatcagcag
    16081 caacgtagtg cagtgacctg tcaccattgt gaagatgcgc cctgcgcccg tagctgccct
    16141 aatggcgcaa tcagccacgt tgatgacagc attcaggtca atcagcaaaa gtgtattggc
    16201 tgtaaatcct gcgtggtggc ctgtccgttt ggtacgatgc aaatcgtcct gacacccgtc
    16261 gcggcaggaa aagtaaaagc cacggcgcat aaatgcgacc tttgtgcagg gcgcgaaaac
    16321 ggtcctgcct gtgtcgagaa ttgcccggcg gacgcgctgc aactggtcac tgacgtcgca
    16381 ctctccggca tggcgaaatc ccgccgcttg cgcaccgcac gtcaggagca tcaaccgtgg
    16441 catgccagta ccgctgcaca agaaatgccg gtaatgagta aagtcgaaca aatgcaggca
    16501 acgcccgcgc gtggcgagcc ggataaactg gcgattgaag cgcgcaaaac cggttttgat
    16561 gaaatttatc tgccatttcg cgccgacctg gcacaacggg aagcctcgcg ctgccttaag
    16621 tgcggcgagc acagcgtttg tgaatggacc tgcccgctgc ataaccatat tccgcagtgg
    16681 attgaactgg tgaaagccgg aaacatcgac gccgccgtcg agctttctca ccagaccaac
    16741 accctgccgg aaattaccgg acgcgtttgt ccgcaagacc gtttgtgtga aggtgcctgt
    16801 actattcgcg atgagcacgg cgcggtaact atcggcaaca ttgaacgcta tatttcagat
    16861 caggcgttgg cgaaaggttg gcgtcctgac ttaagtcatg tcaccaaagt ggacaagcgg
    16921 gtggcgatta tcggggcagg tccggcaggg ctggcctgtg cggatgttct gacccgcaat
    16981 ggcgtggcgg tgacggtgta cgatcgccat ccagaaatcg gtggcttgct cactttcggc
    17041 attccttctt tcaaactgga taaatccctg ctggcacgcc gtcgggaaat cttcagcgcg
    17101 atggggattc acttcgaact caattgtgaa gtgggtaaag atgtctcttt ggattcgctt
    17161 ttggaacaat acgacgcggt cttcgttggc gtaggcactt accgttccat gaaagcgggt
    17221 ttacccaatg aagatgcgcc gggcgtttat gacgcgctgc cgttcctcat cgccaacact
    17281 aaacaggtga tggggctcga agagctaccg gaagagccgt ttatcaatac cgccggactt
    17341 aacgtcgtgg tactgggcgg cggcgacacc gcgatggact gtgtgcgtac tgcgctgcgc
    17401 cacggcgcga gtaacgtcac ctgcgcttat cgtcgtgatg aagctaacat gccaggctcg
    17461 aagaaagagg tgaagaacgc ccgcgaagag ggggccaact tcgaatttaa cgtccagccg
    17521 gtggcgcttg agctgaatga acaaggtcac gtttgcggga ttcgtttcct gcgcacgcgt
    17581 cttggcgagc cggatgccca ggggcgtcgg cgtccagtac cggtggaagg cagtgaattt
    17641 gtcatgccag ccgacgcggt gattatggcg tttggcttca atccgcacgg gatgccgtgg
    17701 ctggagtcgc acggtgtaac ggtagacaaa tggggccgca tcatcgcgga tgtggaaagc
    17761 cagtaccgtt accagaccac caatccgaaa atcttcgccg gtggcgacgc cgtgcgtggt
    17821 gcggatctgg tggttaccgc aatggcagaa ggacgtcatg cggcacaggg gattattgac
    17881 tggctggggg taaaatcagt caaatctcac tgatagcctg cgcagacaaa cccgacttca
    17941 cagcgtaaga taattgttca tttcgcgctg tggagtcggt atgacgcaac aaatcaccct
    18001 cattaaagac aaaattctct ccgataacta tttcaccctg cacaacatta cctacgatct
    18061 cacgcgcaaa gatggcgaag ttatccgcca taaacgtgaa gtttacgatc gcggtaatgg
    18121 tgcgacgatc ctcctgtaca acgcgaagaa aaagaccgtg gttctgattc gtcagttccg
    18181 tgtcgctacc tgggttaatg gcaatgaaag cgggcagctg attgaaacct gcgccgggct
    18241 gctggataac gacgaaccgg aagtgtgcat tcgtaaagaa gcgattgaag agacgggcta
    18301 tgaagtgggc gaagtgcgca aattatttga actgtatatg tcgccaggcg gtgtgactga
    18361 gctaatccac ttttttatcg ccgaatacag tgacaatcag cgcgctaacg ccggtggcgg
    18421 tgtcgaagat gaagatattg aagtgctcga gctgccgttc agccaggcgc tggagatgat
    18481 caaaaccggc gagatacgtg acggtaagac ggtgttattg cttaactatt tgcaaacgtc
    18541 acatttaatg gactgaaaaa taacaataat atttcgttgt ttattattgg ctcaatccga
    18601 caaatccgat tgagccgcgc tacctgcgca acgaagatac gacttgtgct gtttgtttga
    18661 acttctgggg tcgtaccgtc catgcgctat cgcattttcc ttctcttttt tttcgctttg
    18721 ttgccgacgt ctttggtgtg ggcggcacca gcgcaacggg cgttttccga ctggcaggtc
    18781 acctgtaata accaaaactt ctgcgtggcg cgtaatacgg gcgatcataa tggactggtg
    18841 atgaccctga gccgcagcgc cggggcgcat accgatgccg ttttacgtat tgagcgcggc
    18901 ggattgaagt cgccggaggc gtcagaaggg gagatagcgc cacggctgct gttagatggc
    18961 gagccgttgg cgttaagtgg tgacaagtgg cggatttcac catggttatt agtaaccgat
    19021 gatacggcaa ccatcaccgc gtttttgcag atgattcagg aagggaaggc gatcacctta
    19081 cgcgacggca atcagaccat ttcgctgagt ggcttaaaag cggcgttgtt gtttattgat
    19141 gctcagcaaa agcgcgttgg cagtgaaacc gcgtggatca agaaagggga cgaaccgccg
    19201 ctcagcgtac cgcccgcgcc tgcgctgaaa gaggtcgcgg tggttaaccc aacgccgacg
    19261 ccactctcac tcgaagaacg caacgatttg ctggattatg gaaactggcg gatgaatggt
    19321 ctgcgctgct cgcttgatcc attgcgtcgt gaggtgaatg tcactgcgct gactgatgat
    19381 aaagcgctga tgatgattag ctgtgaggca ggggcctata acaccattga tttggcatgg
    19441 attgtgtcgc gtaaaaatcc gctggcctcg cgcccggttc ggttgcgttt gccgttcaac
    19501 aacgggcagg agacgaatga actggaactg atgaacgcaa catttgatga gaaatcgcgt
    19561 gaactggtga ccttagcgaa agggcgcgga ttaagcgatt gtggcattca ggcgcgctgg
    19621 cgctttgacg gtcagcgttt tcgcctggtg cgttacgccg cagaacccac ctgcgataac
    19681 tggcatgggc cagacgcctg gcccacgttg tggatcaccc gttaaacaaa atggcgggga
    19741 catgtaggcc ggataagacg ctgtaggccg gataagacgc gtcagagtcg catccggcaa
    19801 tcagcgtccg gcaatcagca tccggcgatc accatcaggc acctttcact cccagcacct
    19861 tatgcgcttt tgccacaata ttctcggcgg taaagccaaa gaacgggaac agcttatccg
    19921 ccggagcaga ttccccataa cctgtcatcc caacaatcgc ccctttcaaa ccaacatact
    19981 tgtaccagta atcggcaata cctgcttcca ccgccacgcg agccgcaacg ttagaaggca
    20041 acaccgactc ccgatattcc tcatcctggg cgtcgaaaat atcagtcgag ggcagggaaa
    20101 ctacgcgtac attgcgacct tctcccgcta atttctctgc cgcttgcagg gggatttcca
    20161 tctctgaacc ggtggcaatc aaaataatat ctggcttacc gccgctgtct ttcagcacat
    20221 aaccgccacg ggcaatctct tttacctgat ccggcgtacg ttccacctgg gccagattct
    20281 gccttgagag gatcagtgcc gtcggtccgt tgtggcgctc aaccgccagc ttccagccca
    20341 ccgccgcttc cacctgatcg cacggtcgcc aggtgctgaa atttggcgtt aagcgcaggc
    20401 tggccagttg ctcaacagcc tggtgcgtcg gaccatcttc gcccaggcca attgagtcgt
    20461 gggtataaac cataatctgc cgcgctttca tcagtgccgc catccgcgcg gcgttacggg
    20521 cgtattcaac aaacatcagg aacgtggcgg tatacggcac aaagccgccg tggtgcgcga
    20581 tgccgttggc tatagcggtc atgccaaatt cacgcacccc gtagtgaatg tagttgcccg
    20641 ccggatcttc cttcagcgaa acagaacctt tccagatggt caggttgctg gagccagatc
    20701 cgccgaaccg ccgagcaact caggcagcat cggc
//
LOCUS       DADLFA010000058        20714 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000058 DADLFA010000000
VERSION     DADLFA010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20714
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.058"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(73..4533)
                     /gene="mukB"
                     /locus_tag="JQH65_001827"
     CDS             complement(73..4533)
                     /gene="mukB"
                     /locus_tag="JQH65_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HAY6139264.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLDGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVVFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVCADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAAYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLVISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGTDEAPSQAS"
     gene            complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="JQH65_001828"
     CDS             complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="JQH65_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HAY6139265.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDEIEENQPDSGEEE"
     gene            complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="JQH65_001829"
     CDS             complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="JQH65_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HAY6139266.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLQVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="JQH65_001830"
     CDS             complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="JQH65_001830"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HAY6139267.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7458..8237
                     /gene="elyC"
                     /locus_tag="JQH65_001831"
     CDS             7458..8237
                     /gene="elyC"
                     /locus_tag="JQH65_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HAY6139268.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTAKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8214..9107)
                     /locus_tag="JQH65_001832"
     CDS             complement(8214..9107)
                     /locus_tag="JQH65_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HAY6139269.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="JQH65_001833"
     CDS             complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="JQH65_001833"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HAY6139270.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10004..10186)
                     /locus_tag="JQH65_001834"
     CDS             complement(10004..10186)
                     /locus_tag="JQH65_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HAY6139271.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10238..11470)
                     /locus_tag="JQH65_001835"
     CDS             complement(10238..11470)
                     /locus_tag="JQH65_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HAY6139272.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQVITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="JQH65_001836"
     CDS             complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="JQH65_001836"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HAY6139273.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGSKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAEQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="JQH65_001837"
     CDS             complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="JQH65_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HAY6139274.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14379..16547)
                     /locus_tag="JQH65_001838"
     CDS             complement(14379..16547)
                     /locus_tag="JQH65_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HAY6139275.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQQWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     IGRFLQASVIEPNCSLRAQYLASLQTTLQPYMWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRGGQVFLPGRWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLQEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VYDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAITHGDCPLIKLSIAINSKDINGLILHIRVK"
     gene            complement(16754..17038)
                     /gene="ihfB"
                     /locus_tag="JQH65_001839"
     CDS             complement(16754..17038)
                     /gene="ihfB"
                     /locus_tag="JQH65_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HAY6139276.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17198..18871)
                     /gene="rpsA"
                     /locus_tag="JQH65_001840"
     CDS             complement(17198..18871)
                     /gene="rpsA"
                     /locus_tag="JQH65_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAY6139277.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18982..19665)
                     /gene="cmk"
                     /locus_tag="JQH65_001841"
     CDS             complement(18982..19665)
                     /gene="cmk"
                     /locus_tag="JQH65_001841"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAY6139278.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VATFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(19838..20601)
                     /locus_tag="JQH65_001842"
                     /pseudo
     CDS             complement(19838..20601)
                     /locus_tag="JQH65_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
BASE COUNT         4988 a         5722 c         5029 g         4975 t
ORIGIN      
        1 tatgtgccat ttttttagtg cagaaacgga gttttcggaa aaagaaaagg cggcattgct
       61 gccgccttaa ttttaactcg cctgagaagg cgcttcgtca gttcctggaa gcgtttcagg
      121 gagttgcggc gcaaatcctc gcaggccgac gacatgaaca tgttcggtat tctggaagac
      181 tttacgcacc agtttatagg tggtgccttt ctccgggctg atattttccg gcgctgcgat
      241 gatgagctgc atttgcaaac gctcacacaa ttcaaacagc gtggcgatag aacgagcatc
      301 cagtcgcgct gcttcatcga ggaacagcag acggcaagga gagatatctt taccgcgcag
      361 gcggcgagat tcatcttccc agctttgtac caccatcacc agaatcgaca taccggtacc
      421 aatcgcctca ccggtcgaca atgcaccaga ctctgcgcgc aaccaaccat cggaaccacg
      481 gttaacctca acttccattt ccagatagtt gcggtaatcc agcagttctt cgccaatggt
      541 ctgcggcgtg cgctgcccca tatcaatctg cggattaaga cgctgataca atttcgccaa
      601 cgcttccgag aaggtcagac ggttgctgtt aaacagatcc tgatgctgct cgtgctgttc
      661 agagagcaca tccagcagca tggcgtgcgt ttcacgcacg ttcacgttga ggcgcacgct
      721 gttcacctga ccaaacgata cgttctgcaa cccctggttg agcatacgga tacggttctg
      781 ctcgcgctga atggttttgc gaatgatgtt cgccacgctg cgggaactga tcaccagttt
      841 ctgttcacgg gaggttaatt cttcggtcag acggctaagt tcgatctcca tctgttcgat
      901 agcttccacc ggatcatcgg tacgaataat atcctgacga atacgttcac gcagatgctg
      961 ataagccgcc acgaagaact gaattttacg ctccggacgt ttcggatctt ccgacatgcg
     1021 cagcacgtcg cgcagatgtt cgttatccgc caccgccaga cgcagcgcac ccaatgcctt
     1081 atccgacata gagcgcaaat catcagcgga gagataagcc agctcacgac ggtgtaagcg
     1141 gcgctcaacg ccgttatctt tcaccatgcg catcaccgca caccagcccg ctttggcggt
     1201 cactacctgc tcgcgcatct caaagtaatc ccgctccagc ttgcgcagtt tgcgggtcag
     1261 gttgtccatc tccgcttcgc agaaggtaag cgctttttcc agttgattgc ggcgtgaacg
     1321 gttattgctc agttgcgcat gcagctcgtc acggcgaata cgcgcccgct cttctgcccc
     1381 gctatcagca cacacgccga tatcctgcaa ttcacgttgc agatcgttga gtagctcttt
     1441 tttggtgtcg taagaacttt tcagcgaagc cagcacctgg ttgtactgac tcaactgcgc
     1501 tgcgtgaccg cgcaacgctt cgcgagcacg ggtacgctcc gcttccgcct gttccagacg
     1561 ttcacgcagt ttttcgttga gatcgctgtt accgctaagc atttctgccg agtcagagta
     1621 gctaaagtgc gcacgacgct gcaccacttc cgtcagggca aacgcctgct gacgggcatc
     1681 gcgctgcatc tgttgagagt acgcgtaatc ttcttttaac tgttcgaact gttccgggtc
     1741 gctctgcaat accgaaacga tcggttccag tttcgccagt tgattgccaa actgctgaac
     1801 aaaacgcgcg gcttcctggg cttcatccag acgttcgcgg atttcatcga cgcgatccgc
     1861 caggctatca tcagccaaca ggttgagacg cggcagaatg cggttcagcg ccgtaacgcc
     1921 ctctttcgcc tgctcaaact gaatacgctg ctgctggtta tcattttcat gattacttaa
     1981 cgcccgctcc agttcgacgc gacggctgtt cagttgacgg atttctgctt ccgggtcaga
     2041 ctcaaacaca accgccagat gactgccgat aaagcggctg aacgcctgat gcagacgctg
     2101 agttttctgt acatcaaagg agagcgtggc gaagcgttcg gaaagcactt cacgctcggc
     2161 atggaggctt tcaatacggc tttcacgcgc agcacgacca aacagcggca cttccgggaa
     2221 acgtgaataa cgccactgac gatcggcgat tttcaccact accgcttttt ccagctcatc
     2281 aacgctgaac acgctgtcat cgaatgactg cggatctcct tcgatcagat agagatcttc
     2341 cgggcaatcg gtcaagccat ctaggtgttc agttacctgt gacagatctg gcaccacgat
     2401 ggcgtggcgt gacgggccat acagcgctga gaagtacggc gcatcttcca ggctaacgtc
     2461 gtcataaatt tctgacagca gcacaccacc aaaacgctcc gccagcgcgt tcagacgctg
     2521 atcttcagag ccgccaggct ggcttaaacg ttcgatctct tcatcgacgg cgtttttgcg
     2581 cgcgcccact tcatcgcgtt caacaatcgc ctctcgctca cgctccagca actgttgcag
     2641 atattcggtg acgtcctggc tggaggtaaa ctcttcgccg cactgttcgc tcaactggtt
     2701 gagactgttt tgcgctgcca gccaaaccgg cgcacgctgc atcaaactct gaatgcgaga
     2761 ctgcagctgt tcctgctcct ggcgcagtgc catgcgctct tcacgggcgt tagacacgct
     2821 atcggaaaga gaggcaatgc gtgcttccag ttcctgatgc agggcttcca gttcgtcgat
     2881 atcaaaattc ttgccctgac gtttgcagaa atctgccagc agacgctcag cttcttgctg
     2941 ctcgcgtaga cgctgttcca gttcgcttaa tcgcatccgc agcggctgaa cctgctctgc
     3001 caggtgacgc tgatcgaccc cttcgcgcaa tagttcgcga gcgacatccc acgcctcgtt
     3061 acgcgccagt gggccgttga ttgccaccac cagctgataa gcctgctcaa actggctgtg
     3121 cgcggtttgc gccatgctca ttttctgctc aagagagagc attttttcag tcgcttccag
     3181 ctctttcgcc tggaaggttt ccagccattc ggcggcgctg tcggcggtta agtccggcag
     3241 atggcacagt tctttggcac gattaagcgc agcaatcgcc tggttatact ggatcgcgcg
     3301 cgtttgctgg acgtccagcg cctgctggta gtcagcaagc tggcttttca gctcgtccac
     3361 ttccagttcg gcagcttccg cgcgagcctc gttctcttcc tggcgttcga tggcttctgc
     3421 caccacttcg ttttgctctt ccagacggat ctgcagctca tcgagatccg cttcgtagcg
     3481 ttcgatcttc tcctgctgac gcagtgcggt ttgcaccagg ttcaggtgat cgctggccgc
     3541 ctgataatcc gcttccagat cgccttcggc accgttgtgc tctgccagct cacgcgccat
     3601 atcgacgtgt ttgtactgct cagccgccag ttgctgacgc gaagtatgta actcgcgacg
     3661 aaactccagg gctttgtcga gatggacacg gcgctcgttg gcgtgacgca tgtagtccgc
     3721 cgccacgtag ttggtagctt cgctgatcag atgcttaaac aggtcacggt ccgactgggt
     3781 gacacgaatc gcttccagcg tcatgcggtt ttcacgcagc gccgcttcca tgtcctggaa
     3841 cgctttacgc acgccgctgt tttccggcaa cagatagtcg cgcagagaac gggtaatggc
     3901 actggagatc ccgccataca gcgaagcttc gatcagacga tagaatttgc tacggtcaga
     3961 tgcggagcgc agacgacgcg cgatgatgcc cagatcgaac atcagcgagt ggtaatcggt
     4021 aatggagttg aactgtttaa actgcacgcc ttccatcgcc tcgagcttgt ctttcagctc
     4081 gttaagcggc agcacgcgcg cctggcgttc gttcagggtt tcggtcacca gctgtgtcgg
     4141 ctgcaccgac atcggcagtc cctgaatggc aaatggtttg atatccactt tacgatcgcg
     4201 tccggcaacc tgttgcagac gcacaccgac caccacgcgc tggtggcgcg agttaatggt
     4261 gtcgagcatc gaataacaga cacccgcttt cagcttaccg tgcagacctt tatcgcgcga
     4321 accgctggtg gccccggctt ccgtagtgtt acggaaatgc agcagggtca ggtcggggat
     4381 cagcgccgta acgaacgccg ccatggtggt ggatttaccc gccccgttac cgccagaaag
     4441 cgtcgtgacc agctcgtcaa ggtcaaaagt tcgggcaaaa aagccgttcc agttaatcag
     4501 cgtcagtgag cgaaatttac cgcgttcaat cattattctt cctctccgct atctggctga
     4561 ttctcttcga tttcatcgtt gagttgcaga tgattttcaa tcggcattgc ttcgccatca
     4621 cgaatcaggc gacgctgtgc ttcacgggga tcgtcgccag cacgcacatc ggctccgaag
     4681 cggaacaccg attcggtaat gcgaaacttg ctgctgtcgt ggcccataaa ccacaccatg
     4741 cctaaacgac gcagacggtt gagcgaagaa cgtactttct cctgcaactt ctgacggtca
     4801 acgtctgaac cggttgaacg gttgttcacc agtttcagca gttttgcttc atcggccagg
     4861 gtgagcagtt cgtcgtacag ttcctgctgg gtgaaaatcc cctcattcgc cagccgttcc
     4921 gggctgagat agagataaca gaggattttc ccgaccatca tatccagttc cgacaagacg
     4981 gaacgcggga taagagtggt ggaacgtggg cgtaaataga agaacccttc cggtgcgcga
     5041 ataagctcaa cgttataacg cgcgtaaaac tcttccagat attcctgaaa atccatcagg
     5101 aatgcatgat tatccagttc gtcgaggcca atatggcggc ctgaacgtaa ggcgctgtcc
     5161 agcgccggaa ataacggatt cgccagcgcc tgcgccagct taaccggcat cacttgttca
     5221 atatttgtcg atgacatgcg cctgtacctt ggctccgtaa tcattaatcg gctgccattt
     5281 cgctggcagt ccggtgaaat ctgcttgcgc tacgccaaga cgtaccgcct gatcaataac
     5341 aatacgcgca acgtcaaagt gacgtgcacg cggatactgt gacagatatt cgcgtaccac
     5401 cagaccaaga tccagcggca cttgtctggt tttgtacacg gcaagttgtt cttcgatgat
     5461 cgccgccagc tgttcgcgga tctcgttaaa ctcttcgtat tccagatcct caggaagttc
     5521 cccagtcact tcttcatcgc gcagtgccat ctcttcgtca cgcatatcca gcagacgatc
     5581 ggcattggca taagttagcg cccacggctc atcaaaatag gtttgtaccg actgacgtaa
     5641 ccgctgagca aagacgcggt ttttatccat atcgatcgcg gtacgaataa atttgtgtac
     5701 gtggcggtcg tagccaatcc acaagtcgat ggattgctgg ccccaactga taatacgatc
     5761 gagtttgctc tgcagatcga acaccagacg atcgacgaaa tgcagatcgt catgggtcat
     5821 cgtcgcatcc tgaatgcgca acagattagc ctgcaatttg tcgcctgccg cttccagcgt
     5881 atcctgcaat tcacgcagcg ttccggaagt ttccgaaagc aacaattcac agctggaaat
     5941 cgccgcccgc cagtctttgt tcagcaactg ggcgatatcg tccttcacct gctgctgctg
     6001 ttcgtccatc agacgttgcg tcaggtcgat actgtcgaaa atttctgcta ccgaatattt
     6061 cagtggcgca tagacattac ggtgccagtg aaattcatca ccgccctctt cggcggcatc
     6121 tgctgcgcgt ttgagctcac ccgccacaat cgacaactgc atagaaagac gcagcgtaga
     6181 aaactcgcgc tgacggatgt agtagtcagt aatgccgatg ccgagcggcg tcagacggta
     6241 aattgcgttc ccttccgcct gctcgctggt aaagcggttc agcagacgtt gacgcaccat
     6301 gtcgttgatc gcgttattgg cgcgcacgcc gatggtttcg ctggtttgct caaacgcatc
     6361 actcacatgg cggaatgcat ccaccagctc gccttcactc atctcaccat ccagacgctc
     6421 gccgttcagc gtggcaaccg ccagcagaaa agagagtcgg tctacctgca gcgagatgga
     6481 gaagtcattt tttctggccc aggcaaccag ttcggggact gtctgggaaa attcactcat
     6541 actttatcct tgctctgcgg tttgcgcgcg gtgacatgaa tataacgccc cagggtaata
     6601 tacggttcct gacggcaata acgcgtttct aattcaagta atgcttcata gcagtcgcgc
     6661 tgctggtgct tctcgcgcag ataatcatga aacacgcgaa cgcctgtctt acccataatt
     6721 tgccaaccag cttcttccag ccacagataa acctgcgccg ggtcgcgtgg ataatctggc
     6781 gaaagcgtcc gttttttctt tttcggcatt cccgcctgca cgtaatcaaa attcccggcg
     6841 accatgttat gcatcaacaa accatgcgca ttgtagaaca ttaacgacaa cacgccgcct
     6901 ggacgcaaca ctgaccacag ggtctgcaat acactgcggg gatcggccac ccactcgagc
     6961 accgcatgga acaatatcag atcaacgggc gtttccaaat gcgaagcaac atcctgagcg
     7021 gcgcaatgta taaattgcat gttgtcgctc acaccttttg cttctgccgc ctgttttgcg
     7081 cggtcgatca tctgcgcaga aagatcgcat aaaatgacct gatgcccacg ctcggccatt
     7141 ttgattgcgg tctgcccttc tccaccgcca gcatccagca cacgcagttt ttgcgggccc
     7201 atttccgcca gcacgcgatc gagatcctgc cacagaatag cctgtcgaag ctgccctttg
     7261 gtggtgccgt aaatgttacg ggaaaacttt tccgcaatat catcaaaatt gcgatcctgc
     7321 attgctttct ccaccggttg gctaagacgt tttctgataa agacgctatt ttgtcacagc
     7381 cacggcgaga atgaacccgt ctggtgtaat atcgctggcg atcccttgct atatggttaa
     7441 aaaaggaacc agaaaggatg ctttttacac tgaaaaaagt gattggcaat atgttgctgc
     7501 cacttccgct gatgttgtta attattggcg ctggcctggc gctgctgtgg tttagccgtt
     7561 ttcaaaaaac cgcgaagatt tttattagca ttgggtggct ggcactcttg ttattgagtt
     7621 tgcaaccggt agccgaccgc ctgctgcgtc ccatcgaaag cacctatccg acatggaata
     7681 attcgcaaaa agtggactat atcgtggtgc tcggaggtgg ttatacctgg aacccgcagt
     7741 gggcaccgag ctctaattta atcaataaca gtctgccacg cctgaatgaa ggtattcgct
     7801 tatggcgcga aaatccggga tcaaaactga tcttcacggg aggcgtagca aaaaccaata
     7861 cggtgagtac agcggaagta ggtgccagag ttgcgcaatc gctgggcgtg ccgcgcgagc
     7921 aaattatcac cctggatttg ccaaaagata ccgaagaaga agctgcggca gtgaaacagg
     7981 cgattggtga cgcccctttc ctgctggtga cctccgcatc acatctgccg cgggcgatga
     8041 tcttttttca gcaggaaggg ttaaatcctc taccagcccc ggcgaatcag ttggccatcg
     8101 actcgccgct taatccgtgg gaacgggcaa tcccttctcc ggtctggctg atgcatagcg
     8161 atcgtgttgg ctatgaaacg cttggccgca tctggcagtg gctgaaaggt ccgtcaggcg
     8221 agccacggca agagtgattt tgatgccaga tcgaagttgc gccgactaaa tcgtccggta
     8281 ttaaccagtt gcgcaacttc atcccataac acatacaacc aacgccgcca gatgaacgac
     8341 tccgccactg gcgcgcgttg caggtaactc cagagcaaat cttccgccag agaattatcc
     8401 attagtcgga acagttcgta ttctcgcggt gcccaaagca ttagtcccgg cccgaccatc
     8461 gccagtaact gatcgctgcg cgaatctttc agcatgctgc gtaaacagaa gttaccgtga
     8521 atcaacacac aattgtcgtt aaagccttca aataatgccg ggagacattc gcgagtgcga
     8581 aacaggatcc gcttatcctg catcgtcaga ccggtgttat tgaactgatt gagcgtggtc
     8641 catagcactt caacatgttg ccggtaccat gagggccaga aattttcctg agtattgtcg
     8701 accgcgccga cgcaaccgcg actgtcctga cggtgccatg ccagtaaggc ttcaacgatt
     8761 tggtctttga gctgttccca gcgttctggt gttcgggctg gtgcctccac cgaaacacca
     8821 cgcatccgct ccagtagcag gacatcaggg ccggggtgtt cttcatgcgt catcacacca
     8881 taaatagtcg gcatacggac agtcccactt cttgccagca tggtggtttt ccatgccagt
     8941 tgtcgggctt ttccgggcgt actaaagctt cttgccatta acggcattgg gtttccctgg
     9001 ctgtcataca aagcccacaa cgccgtatcc gctttttcat tgacgcactc aatacgactg
     9061 agtttttcgc ccagtaaatg gcttaattcg gcacgcagct gttccattca gattaccctc
     9121 ataaaagcta ctgctttcat aatgagcgcc aatataaaag atgtcacctg aatgagatca
     9181 attgttgaaa agaaaaattg aggaaaatcg taacggtacg acactcctcc caaaattggc
     9241 tgaagtgtcg tgaagtgaaa ttagcgcatt tcagcgcgaa cgcgctcaag atcttcaggg
     9301 gtatccacac ctgtgccagg aacttcctga gcaacagcaa catggatttt ttcgccgtac
     9361 cacagaacac gaagctgctc taacatttcg atgtgttcta acggacttgc ctgccagttg
     9421 acgtaacgac ggataaagcc tgcacggtag ccataaatgc caagatgacg caggaagtta
     9481 tcgccaacgg tttcaaggcc ttctgcaaaa cgatcacgat cccaaggaat ggtggcgcgt
     9541 gagaagtaca gtgcataccc ttcagcgtcg agaaccactt tcaccgcatt cgggttaaac
     9601 gcttcttccg cattgtggat tggcaccgcc agagtcgcca tacccacctg acgctgagcg
     9661 aggttatcag caacctgacg aatgattgtc gcagggatca tcggttcatc accctgcaca
     9721 ttaacgatca ccgtgtcgtc gctgaatgcg catttttcga caacttccgc cagacgttct
     9781 gttcctgact gatgatcggc gcgcgtcata catacttcac cgccagcggc ttcaacggcg
     9841 cgggcaacat cctcatgatc ggttgccacg atgatgcgct cggcacctga ttcacgcgcg
     9901 cgttcaagaa catgaacaat catgggtttg ccgttaatat caaccaatgg tttaccgggc
     9961 agacgcgtgg acgcgtagcg cgcgggaata atgaccacaa aactcatgat ttactctcat
    10021 cagcagtcag cacgcgggct tccgtttcca gcaacactgg aatgccatcg cggagcggga
    10081 aagccaggtt atccagtttg caaataagct cttgtttttc ctggttatac caaagttttc
    10141 cgttgcaaac cgggcaggca atgatttcaa gcaaacgatg atccatagtt cctccagatg
    10201 gatcgggtta tgaatgcata aatcttatca taatcattta tgcgacgggg tctatttccc
    10261 agccagtgcg gcaatcagta aagagacctt gcgggcagcg tcctaatgtc acccgcgttg
    10321 cctgctgcca gctcgcgaaa tcagtaatca cctgacgtaa ccctttttgc agcattgtcg
    10381 ttggtttaat gccttcctgt aaccacagag agataacttc aaggatgcct gtctggcgat
    10441 gcattttggc atccattcgc ccaactaatt gcccacgatg taataatggc agaacaaaat
    10501 agccatactg gcgtttcggc gctggggtat agcactccag ccggtagcta aaatcaaaaa
    10561 gctgctctgc gcgtttgcga tcccagacaa caggatcaaa aggcgaaagt accgcgctgt
    10621 gcgttgcagt gagctttccg gccagcgcac gctcgagtag cggcagcaaa tcagcatgca
    10681 gccaaagatt gcccaatttt tcaacatgca cagcaatgat ttgctgctgt tcagccctcg
    10741 cttcgcgcca cgccgccagt gccggacgtt tcagccgata gtaatctgcc agccactgtt
    10801 cgcggaatat tcccaggcta cgcgcactgt tatccagcat gatgatttct gcttctgttt
    10861 gcgaaacgag atcgcgctca tcatcccagt caggcatgac acggtgggtt aaatcataaa
    10921 cgcgctggaa gttgcgccgt tcaatcacca tcacctttcc ggcagtaaat aaaccttcca
    10981 gatgccgttt atgcggtttc cattcccacc agccgcttgc acctttacga ggatgctcaa
    11041 aatcggctga acgtaccggc cccttatcat gaatatgctg aattaactgt gcaatttccg
    11101 cctcatgttc ctgcatccag gcgtctttgt atttccagcc cattttttca ggtgccagca
    11161 tgcggtggcg aataagacga aagtcgctac gcggcataaa gcaggcttca tgcgcccagt
    11221 attccattaa ttcgccacgc gccagagact catccagcca ctgggcagga taatttccca
    11281 gacgactgaa aagcaccaga tatggactac gggcaacaat attgatggta tcgatttgca
    11341 gcaaggacat gcgggagatc gttgccggaa tatcctccaa cgacgctcga cggcggggtt
    11401 tgtttaacag gccttgtgcg gcaaggtgaa gattacgcgc atcagcaagg gagaggtgcg
    11461 gcagcgacat tcatgactcc atcaatcgaa cgctgccgcg gcgtaactag ttgccagaag
    11521 ccagcgaggt tagttgcgta agcagtttcg ctggttcatc acctgaaagc tgtgcgtcta
    11581 caggcaaata ccaccaattt tcttctgcaa aggcccggca tttcaccgca tctttttcag
    11641 tcattaccag cgtttgctcg gcgcttacca acgcactgac atccgcatgg ttcaaagact
    11701 gatgatcggc cagcggtaca catttttccg gttgtacgcc acacatcttc agcgtggcaa
    11761 aaaagcgcgg cggatgccca atccccgcca tcgccactac atgttcaagc tgagcaacgt
    11821 cacaacgcgt accggtacgt aaattcaccg cctgacccgg cagcagatgc atgggaattt
    11881 caccgctgcg agggacaccg ccgttgacga ttaccgcatc aaccgacttt aagcgccccg
    11941 ctcgctcacg cattggcccc gccggcaacc accagccatt gccaaagcga cgcacaccat
    12001 caataacgac aatttccaca tcacgcgcca gacggtaatg ctgtaaaccg tcgtcggtta
    12061 cgatgatctg cacatcaggg tgttgcgcca gaatggcttt taccgcatca gaacgaacgg
    12121 gagaaaccgc aacaggcgca tcagtgcgtt gataaatcaa cacaggttca tcacccgcct
    12181 gtgctgttgt ggtatctgcc gacaataaca gcggataaga ttcagcctta ctaccatatc
    12241 cccgcgatac gacccccacg cgaataccgc gctgttgcaa ctgttccacc agccagacaa
    12301 cgaccggggt ttttccgttg ccgcctgcgg tgagattacc aaccacgaca accggtacgg
    12361 gggcacgcca ggcgcgcttc agttttagtt tatagcaaag acggatcgcg ccactcacca
    12421 ggccatacaa ccaggagagt ggcagcaata gccgccacaa aggggattca ccagaccaga
    12481 ttttttcgat cattggccaa actgcatttt gtgaagttgc gcgtaaacgc cgcggtgctc
    12541 aagcaaatcg ttatgcgtac cgcgttccac aatgacacca tcctcgacga ccacgatttc
    12601 gtctgccttt tcaatggtag acaagcggtg ggcaatcacc agagaggtac ggtttttctg
    12661 caactcatcc agtgccgcct gaatcgcacg ttcggattcg gtatccagag ccgaggtagc
    12721 ttcatccaga atcagaatcg ggctatcacg cagcaaggct cgagcgatag caatacgctg
    12781 acgctgaccg ccagagagca gcacgccgtt ttcaccaatc actgtatcga gaccgttatc
    12841 catcttattg atgaagtcca tggcgtaggc catacgcgcc gcttcttcaa tttgctcacg
    12901 gctgtactgt tcagtccgtg cgtaagcaat gttgttagca accgtatcgt taaacagatg
    12961 gacattctgc gacaccagag caacctggtt acgtaacgac gccagggtat actcgcgcag
    13021 atcgtgacca tccatcagga tttcgccttc atcaatatcg taaaaacgag tgatcaggct
    13081 ggcgatggtt gatttacctg aaccagagcg tccaaccaga gcaaccgtct tccctgccgg
    13141 aattttcagg ttgatgttac gcaatgcagg tacgtcacgt cccggataag taaaggtgac
    13201 attgcggaat tccacgtcgc cagtcgcacg ctcgatcacg cgcttacctt cgtctttctc
    13261 ctgctcactg tccagaatgg taaacagcgt ctgacaagcc gccataccgc gctggaactg
    13321 ggcgttaacg ttggtcagtg atttcagcgg acgcatcagt gcaatcattg aagagaaaac
    13381 aacggtaatc gtaccagcag tcaggctatc catgacactt gggaagctcg ccgcatacag
    13441 aacaaacgcc agcgccaaag aggcgatcag ctgaatgatc ggatcagaga tggaagaggc
    13501 tgaaaccatt ttcatcccct gaagacgcat tcggttgctg actttatcaa agcgtttcgt
    13561 ttccacttcc tgaccaccga aaatcaatac ttctttgtgg cccttcagca tttgttcggc
    13621 gctggtggtc acctgcccca tggtgttctg catgttttta ctgatgttgc gaaaacgctt
    13681 cgatactacg cgaatcgcaa tcgaaacaat cggtgccagc acaatcaaaa tgatcgacag
    13741 ttgccaactg taatagaaca tcatgatgaa caggccgatg attgacgcac cttcacgcac
    13801 aaccgttatc agtgcgccgg aagaagaaga agcaacctgt tcggaatcgt aggtaatacg
    13861 tgacaacagc gtccccgttg actgtttgtc aaaaaatgaa accggcattc ccatcatgtg
    13921 accaaacagg cggcgacgca tggtcattac cacctttcct gatacccagg agatacagta
    13981 gctggagaca tagctggtga taccacgtaa aatcatcagc ccgatcacca ccagcggcat
    14041 ccacaccagc acggagcgat ctgttttacc aaagccatca tcaagaagtg gcttaaggag
    14101 cgataacatg aaggtatcgc tggctgcgtt gaggattaac gctacgcccg ccacgatcag
    14161 acccgcttta aaaggcgcaa tggttggcca cagtcggcgg aacgtctgcc acgtagagag
    14221 atctttgtcg ttatgcattc aaaaaaccag catttgttga aatagccgca tattctaccc
    14281 gttatccact ggcacgccaa accactgatg ataccaacga ggtaaaattt gctctctgag
    14341 gctgctaatc cgccagcctt gcgctgaaaa attgaccgtc acttgaccct gatgtggagt
    14401 atcaagccat tgatatcctt gctgttgata gcgatgctta actttattag agggcaatcg
    14461 ccatgcgtta tagcgttata gcgcgatgcc gatgcgagtg ccacttttcc attcactcgc
    14521 tgaattaatg gcaacgatga tgaggtatta ctgccatggt gaggtacctg aagcaatgtt
    14581 gtctgcactt gctgccagta acggcttagc atcttttgtt cagcggggac ttcaatatca
    14641 ccggttagaa gaatgctatt cgtcccgtca taaaccttaa ccacacagga atggttattt
    14701 cctttatcgt tgctagcttg taaaggccag tgcacgctga aacgcaatcc ttgccattgc
    14761 cacgcttcgc cacgcacaca gggctgatga tgttcccagt ttaacggact tctgatccat
    14821 aacatcggcc atgtgtgcaa tattgagtcc agccctcccc ggtgatccag atgttcatga
    14881 ctcagaataa cgccttccgg ttcaagatta tgccagtgga gccaggggat aatcagttgt
    14941 tgcccactat ccccttcggg ccaggccagt cctgtgtcat agagaatcgc tttgccgttt
    15001 ctggctatca ccattgccag cccttgcccg acatcaagca tgtacacctg ccactcgtca
    15061 ggtcgaggtt tttgccacag cggccagcac atcagcaagc ctacagccac acacattgct
    15121 ggcaacgttc gccaggcgtt taatcgccat accaccagta agaaccatgg ggaaaatgat
    15181 agccattgcc aacgttcagc aatgttgatc cacccttctt gcaaactctt taacccccag
    15241 aaaagtaaag ccaaagaccg gtcggcaaga aaccataacc cttgctccag gattaacggc
    15301 ccgcttaaat gcacaaccat cgcggcgagg atcaacggaa ccgtgataaa tgtcaccaag
    15361 ggaattgcta atagatttgc aataaacgag gtcagactaa tgccatgaaa tatgacgatt
    15421 tgcacaggca taagcaggag tgtgattccc agttgcaaat gaatgaggga aacaactgca
    15481 cgcaataccg gcggcagttg ccactcagga cagggaaacc actggtacca aaaaatcagt
    15541 gccgcgaccg cagcggcaga gagccataaa ctttgcgaga gaatggcaac aggatccatc
    15601 agcaaaattg ccgccagaca acatatccat acatcccagc cactccactg tcgcccactt
    15661 aacttaagca ttccccacgt agcaagcgcc accacggtac gcaatgcagg aggttgcatt
    15721 cccgtcaacc aggcataaaa agcagcacag cagattccac caattaatgg catttgccag
    15781 tggatccagc gcccaggcag aaaaacttgc ccaccgcgaa tgagtccggc agccagcaac
    15841 gccgcaaaag cgatgtgcaa tcccgatatc gccattaaat gcgccgttcc agtatcacgc
    15901 atgatatttt tgatttcttt ggggacggat aaccgttccc ccatacctaa accaagaata
    15961 accgcattcc acatataggg ttgcagcgtt gtttgtagtg acgccagata ctgtgcacgc
    16021 aggctacaat tcggttcaat gacacttgcc tgcagaaagc ggccgatgag cggctgatgc
    16081 tgggcaatgg cataacgctg gctatcaaag ccgccatcat taagttggcc atgaactgca
    16141 cgaactttga gtttcattga ccattgttgt ccagcacaaa ccgcttgcgg aagatattcg
    16201 ccatacagca caagacctgg cgcagggaat atacgtcgac cttgtagatg agtaatttga
    16261 ccgtaatgag tggtcatgcc gtcagttgca gtgatctcaa caattgcatc ctgcgtcgcg
    16321 ccagttaagg tttctcctgc ccacaaaatt tgctttgctg ataatatgcc ccaaacgaaa
    16381 aacagcaacg tcagcgcgat ataacggacg gttttaacag ggataaatgc cagtacgcag
    16441 gcgaagagag tcagaaacgc aagggttaat gtcccaggca attggggcaa aatcagcaac
    16501 ggaaaaattc cgcaaattat gcatacgccg actgtcgtta ttttcatgca cccctccctg
    16561 tttatggaaa gtgtgcaact ttgttgagcg gttgtctggt gggaattttg cgttccccga
    16621 ggcacattca gaagaattta acgaaagggt gaaaactgct caaaactctg cgaggcagct
    16681 tccagttcct gattagagaa atgaaaaaag cacccgacag gtgcttttct cgcgttcaag
    16741 tttgagtaaa aacttaaccg taaatattgg cgcgatcgcg cagttcttta ccaggtttaa
    16801 agtgaggaac gtattttcct tccagttcta ctttatcgcc agtcttcgga ttacgtccgg
    16861 tacgtggtgc gcggtagtgc aaagagaaac tgccgaaacc gcggatttca atacgctcgc
    16921 cctgcgcaag agtcgaggcc atatgctcca gcatctcttt tactgcatct tcaaccgtct
    16981 tggcgggaat gtgcgattgc tgggtggcaa gtctttctat caattctgac ttggtcatga
    17041 ttcctccggt tccttaaagg caaattaagc ggctgctgtt gctgcattga ttaagggcgg
    17101 ccgtagccgc ccttagtgct tgattacagg acgaaacctg caatctgtca agtaaactca
    17161 acaaacttcg gaataaaaat cccgaagagt cagagaatta ctcgccttta gctgctttga
    17221 aagcttcagc cattgcgttg ttggagaagt ttgcatcttc ctgtttgtta acagttgcga
    17281 ttgcatcttt ctcgtcagct tcgtctttcg cacgaacaga caggctgatt gcgcggtttt
    17341 tacgatcaac gccggtgaat ttagcttcaa cttcgtcgcc aacgctcaga actagggtag
    17401 cgtcttcaac gcggtcacgg gatgcttcag aagcacgcag gtaaccttca acgccgtcag
    17461 ccagttctac ggttgcgcct ttagcgtcaa ctgcagttac tttaccggtt acgatagcgc
    17521 ctttcttgtt cagagcaacc cagttgttga acggatcttc tgcgagctgt ttaacgccca
    17581 aggagatacg ttcacgttct gcgtcaacct gcagaacaac tgcagcgatt tcgtcgcctt
    17641 ttttgtattc acgaactgct tcttcgcctg caacgttcca ggagatgtca gacaggtgaa
    17701 ccaggccgtc gatgccgccg tctaggccga tgaagatacc gaagtcagtg atagacttga
    17761 ttttaccttc aacacggtcg cccttgttgt gggtttccgc gaactgctgc cacgggttag
    17821 ctttgcactg tttcagaccc agggagatac gacgacgttc ttcgtcgata tccagaacca
    17881 taacttccac tacatctcca acgttaacaa ctttggacgg gtggatgttt ttgttggtcc
    17941 aatccatttc ggaaacgtgt accaggcctt caacgccttc ttcgatttca acgaagcagc
    18001 cgtagtcggt caggttggtc acgcgaccag tcagtttggt accttccgga taacgtttag
    18061 cgatagctac ccacggatct tcgcccaact gtttcaggcc cagggataca cgggtacgtt
    18121 cgcggtcgaa cttcagcact ttaacagtga tttcgtcgcc cacgttgacg atttcgctcg
    18181 gatgcttaac gcgtttccag gccatgtcag tgatgtgcag caggccgtca acgccgccca
    18241 gatcaacgaa tgcaccgtag tcagtgaggt tcttaacgat acctttaact tccatgcctt
    18301 cctgcaggtt ttccagcagc tgatcgcgct ctgcgctgtt ttcggattcg ataacggcac
    18361 gacgagaaac aacaacgttg ttgcgcttct gatccagctt gattacttta aattcaagct
    18421 ctttgccttc caggtgcaga gtgtcacgca ccggacgaac gtctaccaga gaacctggca
    18481 ggaacgcacg aataccgttc agctcaacag tgaagccgcc cttaactttg ccgttgataa
    18541 caccggtaac agtttcagca tcttcgtaag ctttttccag cgtgatccag gcttcgtgac
    18601 gtttagcttt ctcacgggac agcagagttt caccgaagcc gtcttctact gcgtccagag
    18661 caacgtcaac ttcgtcacct acctggattt ccagctcgcc ctgggcgttt ttgaactgct
    18721 cagccgggat ggcggactca gatttcagac cagcgtcaac cagtactacg tctttgtcga
    18781 tagcaacaac aacgccacga acgatagaac ccgggcgggt ttcgatttct tttaaggact
    18841 cttcaaagag ttgagcaaaa gattcagtca tgtttaatct tcaggtttat atttaacgtc
    18901 cacctggctc catgccggat ggggttgttt aacatacccg ctgtcattcc attgcaacgg
    18961 gggtactgca aattcggtcg cttatgcgag agccaatttc tggcgcgcgt attgtagcgc
    19021 tttttcaatc acttgctcaa tgcttaaggt ggtggaatcc aacactaaag catcggctgc
    19081 cggaaccagt ggcgctaccg ctcggttacg atcgcggtcg tcgcgttctt tgatctcggc
    19141 caaaaggcgc tcaaagttaa cactaaagcc cttctcctgc aactgtagca tgcggcgatg
    19201 cgcacgttct tccgaggagg cgtcaaggaa aattttcact ggtgcatcag ggaataccac
    19261 cgttcccatg tcgcggccat cggcaatcag acctggtaat tcgcgaaacg cgcgttggcg
    19321 acgcaataat gcttcacgaa cgcgtgggaa tgtcgcgact tgtgaagccg cattcgccac
    19381 ttcctgagta cgaatttcgc cgctgacatc ttccccttcg aggatcactt ccagattgcc
    19441 attggttgac acaaaacgta catccagatg ggatgccagc ggtaccagcg catcttctga
    19501 cgcaacatca acatggtgat gtaatgccgc caatgccagt acgcgataaa ttgcacccga
    19561 atccagcaga tgccattgca atgcttccgc catagcctta cacaaggtgc ctttccctgc
    19621 accgcttggg ccatcaatgg taataaccgg ggcaattgcc gtcatcttta tctccttaaa
    19681 acaggcgtac cgttaacata accgcgcgca ttatacgcgc cgacgccaca aatcgttaac
    19741 gttgtgtgca aaaaccagtt ttgtctgtat tcagatgggg atttattgcg caattatagc
    19801 gggctggcag aagaccagcc cgaaagatga caatgattta cttaccagag gcgatacgat
    19861 cacggatgtg ttgcgcacgc tctgttgatg gtgggtgaga gtcaaacatg gactgggtgc
    19921 gaccgccatc cagactagcc agtttttcaa agctgccaac cagcccctag gtgctgatgc
    19981 cacgtttctt caacagatca aaggagaaat catctgcttc ggactcttta tcacgggagt
    20041 atttagcatt aatagcgcct tctgcgatat cacccaattg tgagcgggaa agctgggaag
    20101 ccacaccgct ggtagctgaa atggcatcgc gtgcggcaac gatcgcataa gaaactttca
    20161 tttcagccag cgagtgaccc aacgcgacgt ggcccagttc atggcccaga acaccttcaa
    20221 tttcgttgtc attcatcatg tccatcaggc cactgtagac acggacacag ccgttcgcca
    20281 tcgcccatgc gttgacgtcg ctggtcatat aaacctatag ttgaccggcg tgccattaat
    20341 attgttaccc aatgctttgg cgatttttgc cagacgttta ccgtacttgc tggatttact
    20401 tgcgacttga ttgccgctgt ccatttcggc acagccctga ttggcaatcg cttttgcatc
    20461 ggcatcgctt aatgttgctg ctttataggc atttaaaccg gagctgatag ccatattggt
    20521 atcaataccg tgggtatttt gacaccctgt cagtaatgct gcagaggtcg caatcgccag
    20581 cagtaattta gtattcttca tgtgaatcat ccgttgatta atcatatata aacattatgt
    20641 atcaacaact tacattgaca agcgacgcta ccattaatca aaatgactca caaggtccga
    20701 aaaaaaacgc cgac
//
LOCUS       DADLFA010000059        20685 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000059 DADLFA010000000
VERSION     DADLFA010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20685
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.059"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..257)
                     /locus_tag="JQH65_001843"
     CDS             complement(<1..257)
                     /locus_tag="JQH65_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HAY6139279.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGICTGEGLEIVGQGDC"
     gene            complement(248..718)
                     /locus_tag="JQH65_001844"
     CDS             complement(248..718)
                     /locus_tag="JQH65_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HAY6139280.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHICFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(903..1697)
                     /gene="thyA"
                     /locus_tag="JQH65_001845"
     CDS             complement(903..1697)
                     /gene="thyA"
                     /locus_tag="JQH65_001845"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAY6139281.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYAPHPGIKAPVAI"
     gene            complement(1704..2579)
                     /gene="lgt"
                     /locus_tag="JQH65_001846"
     CDS             complement(1704..2579)
                     /gene="lgt"
                     /locus_tag="JQH65_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAY6139282.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(2730..4976)
                     /gene="ptsP"
                     /locus_tag="JQH65_001847"
     CDS             complement(2730..4976)
                     /gene="ptsP"
                     /locus_tag="JQH65_001847"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAY6139283.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGLPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPILLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(4989..5519)
                     /gene="rppH"
                     /locus_tag="JQH65_001848"
     CDS             complement(4989..5519)
                     /gene="rppH"
                     /locus_tag="JQH65_001848"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HAY6139284.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNTSAYRRKRG"
     gene            6204..6893
                     /gene="mutH"
                     /locus_tag="JQH65_001849"
     CDS             6204..6893
                     /gene="mutH"
                     /locus_tag="JQH65_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HAY6139285.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWTPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            6962..7675
                     /locus_tag="JQH65_001850"
     CDS             6962..7675
                     /locus_tag="JQH65_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HAY6139286.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            7813..8031
                     /gene="ygdR"
                     /locus_tag="JQH65_001851"
     CDS             7813..8031
                     /gene="ygdR"
                     /locus_tag="JQH65_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HAY6139287.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            8139..9179
                     /locus_tag="JQH65_001852"
     CDS             8139..9179
                     /locus_tag="JQH65_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HAY6139288.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPSIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKTAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(9211..10404)
                     /gene="lplT"
                     /locus_tag="JQH65_001853"
     CDS             complement(9211..10404)
                     /gene="lplT"
                     /locus_tag="JQH65_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HAY6139289.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNSTPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(10397..12555)
                     /gene="aas"
                     /locus_tag="JQH65_001854"
                     /pseudo
     CDS             complement(10397..12555)
                     /gene="aas"
                     /locus_tag="JQH65_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417313.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
     gene            12944..13975
                     /gene="galR"
                     /locus_tag="JQH65_001855"
     CDS             12944..13975
                     /gene="galR"
                     /locus_tag="JQH65_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HAY6139290.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(13982..15244)
                     /gene="lysA"
                     /locus_tag="JQH65_001856"
     CDS             complement(13982..15244)
                     /gene="lysA"
                     /locus_tag="JQH65_001856"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAY6139291.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSVPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRSLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            15366..16301
                     /gene="lysR"
                     /locus_tag="JQH65_001857"
     CDS             15366..16301
                     /gene="lysR"
                     /locus_tag="JQH65_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HAY6139292.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASICAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(16288..16980)
                     /gene="ygeA"
                     /locus_tag="JQH65_001858"
     CDS             complement(16288..16980)
                     /gene="ygeA"
                     /locus_tag="JQH65_001858"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HAY6139293.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(17109..18524)
                     /locus_tag="JQH65_001859"
                     /pseudo
     CDS             complement(17109..18524)
                     /locus_tag="JQH65_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
     gene            complement(18839..19600)
                     /gene="kduD"
                     /locus_tag="JQH65_001860"
     CDS             complement(18839..19600)
                     /gene="kduD"
                     /locus_tag="JQH65_001860"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HAY6139294.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(19630..20466)
                     /gene="kduI"
                     /locus_tag="JQH65_001861"
     CDS             complement(19630..20466)
                     /gene="kduI"
                     /locus_tag="JQH65_001861"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAY6139295.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLHNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
BASE COUNT         5298 a         5533 c         5018 g         4836 t
ORIGIN      
        1 atacagtcac cctgtccgac aatttccagc ccttcgccgg tacagatgcc atgacaataa
       61 cccgccctct ggagatgctt cgcgacggta aatacccgca gccagatttc atcttccagc
      121 gccagcttac gggtgctcgt taaactttca cgctgtaacg caggcagaaa gcgtgcagcc
      181 cccagcaaca atacgctact gatcgccata gcaatcaaca cttccagcag agaaaaacct
      241 tgctctttta caggcatcct tctgtttctc cttgctgaca aagccggagt cttccccacg
      301 gcgaaaccac cagccaccac tcgcccgttg agtttttgaa gcaaatatgc ccggcccatg
      361 cggtattgcg caggccaaag aaagcaagcg aaggtgtcag gtcgctcatt tcgacttcgg
      421 gccagcgtgg cacaaagacc aatggtgaac tgccatgaca ggtattggcc ccagcagcgg
      481 aactcacaag gcaccataac gtcccctccc tgataacgct gatactgtgg tcgcggttat
      541 gccagttggc atcttcacgt aaatagagca aatagtcccg cgcctggctg gcggtttgcc
      601 atagccgttg cgactgctgc cagtattgcc agccatagag tccacttgcg cttagcatga
      661 ccaaaatcag catcgcgacc agcgtttcaa tcagcgtata accacgttgt gttttcatgc
      721 cggcagtatg gagcgaggag aaaaaaagac gagggccagt ttctatttct tcggcgcatc
      781 ttccggacta tttacgccgt tgcaggacgt tgcaaaattt cgggaaggtg tctcgaagaa
      841 tttaatggag ggtaaaaaaa accgacgcac actagcgtcg gctctggcag gatgtttcgt
      901 aattagatag ccaccggcgc tttaatgccc ggatgcggag cgtagccttc aatctcaaaa
      961 tcttcgaaac ggtagtcgaa gatggattcg ggtttacgtt tgataatcaa cttcggcagc
     1021 ggacgcggtt cgcggcttaa ttgcagatga gtttgatcca tatgattgct gtacaggtgc
     1081 gtgtcgcccc ccgtccagac aaaatcgccc acttccagat cacactgctg cgccatcata
     1141 tgcaccagta acgcgtagct ggcaatgtta aacggcaggc cgaggaaaac gtcacaggag
     1201 cgctgataaa gctggcaaga gagtttgccg tctgccacat agaactggaa gaacgcatgg
     1261 cacggtgcca gcgccatttt atccagttcg cctacgttcc acgctgaaac aataatgcgg
     1321 cgcgaatccg ggtcgttttt cagctggttc agtaccgtag tgatctggtc aatatgacga
     1381 ccatccggcg ttggccaggc gcgccactgt ttaccataca ctggcccgag gtcgccgttt
     1441 tcatcggccc attcgtccca gatggtgaca ttgttttcgt gtagataagc aatgttagtg
     1501 tcgccctgca gaaaccacag cagttcatgg atgatggaac gcaggtggca acgtttagtt
     1561 gtcaccagcg ggaatccatc ctgcaggtta aaacgcatct gatgaccaaa aatggaaagc
     1621 gttccggttc cggtacggtc gtttttctgt gtgccttcgt cgagcacttt ttgcatcagt
     1681 tctaaatact gtttcatggt tcctcaggaa acgtgttgct gtgggctgcg acgatatgcc
     1741 cagaccatca tgatcacacc cgcgacaatc atcgggatgg aaagaatttg tcccatgctg
     1801 atgtactgca cccaggcacc agtaaactgc gcgtcgggct ggcggaaaaa ctcaacaatg
     1861 atgcgaaacg cgccgtaacc aatcaggaac aaacctgaga cagctcccat tgggcgcggt
     1921 ttacgaatat acaggttgag gataataaac agcactacac cttccagcaa cagctcgtaa
     1981 agctgtgatg ggtggcgcgg cagcacaccg taagtatcga aaatggattg ccactgcggg
     2041 ttggtttgca gcagcaaaat atcttctgta cgggagccag ggaacagcat ggcaaacggg
     2101 aagttcgggt caacgcggcc ccacaattca ccgttaataa agttgcccag acgcccggca
     2161 ccaagaccaa acggaatgag tggtgcgata aaatcagaga cctggaagaa ggaacgttta
     2221 gtacggcggg cgaagataat catcaccacg ataacgccaa tcaggccgcc gtggaaggac
     2281 atgccgccgt cccagacacg gaacagatac agcggatcgg ccataaactg cgggaagttg
     2341 tagaacagaa cataaccaat acgtccaccg aggaagacgc cgaggaagcc cgcatagagt
     2401 aagttttcaa cttcattttt ggtccagcca ctgcccggac gattcgcccg acgtgttgcc
     2461 agccacattg caaaaatgaa acccaccaga tacatcaggc cgtaccagtg aagcgccacg
     2521 ggtcctattg agaaaatgac cggatcaaac tccggaaaat gcagatagct actggtcatc
     2581 tgtcaccaca agttcttgtt atttcgctga aagagaacag cgattgaaat gcgcgccgca
     2641 ggtttcaggc gctccaaagg tgcgaataat agcacaaggg gacctggctg gttgccggat
     2701 accgttaaaa gatatgtata tgatccgcgc tataaccctc cgcgaatcag cccgcccatg
     2761 ccgcgacgct ccataaaggc tgcaacctga tgacgaactt cggtcgccag ttgcgcttcc
     2821 agactacgct gcgcaagatt ttctgcttcg gcaaaatcaa tgcgccgcag caggtatttt
     2881 acccgcgcta cagaacgtcc gttcatagac agatggcgat acccaagccc aatgaggatt
     2941 gccacgcaca tgggatcgcc cgccatttca ccgcacaaac ggagatcgat accatgtatt
     3001 tccgcttccc gggcgatcat tgccagagct cgtaacattg caggatgaag gctgtcataa
     3061 atgttcgcca cccgggtatt gttgcgatca acggccagaa tgtattgagt cagatcgttg
     3121 gtgccaacag agatgaaatc gacccgcttt gccagatgcg gcagcataaa taccattgac
     3181 ggtacttcca gcatgatgcc gatacgtggt ttgggaattt cgtaaccgat catctcctcg
     3241 acttcacgtc cggcacgttc aatcaggcgg cgtgcttcgt caacttcatc gaggctggtg
     3301 accatcggca acagaatatt caggttgccc gtagcggcat tagcacgcag catcgcccgc
     3361 acctggatca agaagatctc cggctgatcg agcgtaatgc gaatcccacg ccaacccagg
     3421 catggattct cttcgctgat cggcatgtaa ggcagctgct tatctgctcc gacatccagc
     3481 gtacgcaagg tgacgggttt atcattgaac atttgcagca tcccctgata ctgcgccacc
     3541 tgttcttctt ccgacgggaa accactttgc agcatgaatg ggatttcagt gcgataaaga
     3601 ccgatgccat caatacggct gcccagtttt tcttcatgtt ccgggcttaa accagcattg
     3661 agcatgactt ttatacgctc accgcttttt aactgcgcgg gtaaattgac gtcatcttcc
     3721 gccagacggc taagctcaat ctcttcacta attagccgct gatattcttg cagcagtatc
     3781 ggctccggat cgaccagcaa ttcaccgcga tagccatcaa cgatcagcgt cctacgatgc
     3841 agcaccgaag gctgaatatc cgcgcccatc acggtaggga gccccagcgc acgtaccatg
     3901 atcgcagcat gggagttggc tgcgccatct cgcacgacaa caccgactaa gcgatcctgg
     3961 ggcagctcag caagagttgt cgctgacagt tcatctgcca ccagaatgaa acgttccggc
     4021 caggcgttcg gcccttgatt agcgtcatca agatgaaaca gcaatcgctg acccagcgca
     4081 cgtaaatcgc cagcccgctc tttgagatag ttatcgctta gcgcggcaaa ctgttcggca
     4141 aatttttcaa tgaccgtttt taccgcccac tctgccaccg agcctttatc aacctcggca
     4201 aacaattcgc gacgcagccg ggtgtccgaa agcaggtgcg agtaaagatc gaaaatagcc
     4261 gccgtttctt tttgtgctcc ggcggcaaag cgtttgctgt agcggcgaaa ctcgtttgcc
     4321 gcttcttcca gcgccccggt caatcgttcg cgttccagag ccggatccag cgttgatgcc
     4381 tgatacacct gttccattaa aggtaacgtg gcatcctgcc agccttcggc aatcgccaca
     4441 ccaggtgctg ccggtaatgc gcggattcgc gtctggcgat attgcccaaa caaggcagtc
     4501 aactgcgact gagaaagaat agctgccatc tgggtggcaa gcgtcaccag gaaggattct
     4561 tcactttcgt catactggcg caactctcgt tgctgtacca ccagtacacc aagcaactgg
     4621 cgacgttgaa taattggtac gcctaaaaac gcgcggaaac gttcttcttt tacggagggg
     4681 atgtatttga agctggggtg cttttgcgca tctgcaaggt ttatcggttc cgccagcctg
     4741 ccaaccaggc cgacgatccc ttcatcaaac gcgagcgtta cagtgcgacc gcgtggtttt
     4801 ttcagccccc gggtcgccat caggtagtaa caacgtcgat catgatcggc caggtagacc
     4861 gaacagacct cggtatccat cgcaagacag atgtcggtaa ccagaatatt taacgcctca
     4921 ttcaggcgtg gtgcgctggc taccttttcg actatttcgc gcaggcgagt gagcataatt
     4981 ggcgtgactt aacctctttt acgtcgataa gcagatgtgt tttgtggttt tggcgtattt
     5041 tcctgcagtg acatcaccac actcgcgaac tctttcatta ccctacggta gacatcacgt
     5101 ttaaatgaca ccacctgtct gaccggatac cagtaactta cccatcgcca gccgtcaaac
     5161 tctggtgtac tgctggtttg catattgatt tctgcatcgc cgctcaccag ctgcaagaga
     5221 aaccattttt gtttttggcc gatacaaacc ggcttcgtgt cccaacgcac caaacgtttc
     5281 ggtaatttgt agcgcaacca gttacgcgtt gaagcaagga ttcgaacgtc tttgcggctt
     5341 aatcctactt cttcaaacaa ttcacggtac atcgcctgct ctgcggattc tccggggttg
     5401 attccgcctt gcggaaattg ccaggagtgc tgaccaaatc gccgggccca cattacctgc
     5461 ccctggcgat tacaaatcac gatacctacg tttgggcggt agccatcgtc atcaatcacc
     5521 ggactacctc aaaataaagc tttatatacg aatgattgtt tcatactcca ggaagacggt
     5581 aaaccactct ctgcagggca ttacacacta ataacaattg aataactcac agttatgtgc
     5641 agagttataa acagaggaag gggtggatag ccgtttttat tcaccttttc tgtggataga
     5701 gttgtgaaga actacggaat tactacggga aaacccggag cattctgaat aagcccgata
     5761 taggcatttt aaaatattca ttataagtca atgcattaca tgtaatttga gtaatgaaaa
     5821 agagtaattc gtgacccagg tcacacctct catttacggg ttggcgaaag atcaaccaat
     5881 gccgtattta tccacagaat gtgccactaa gttaagcact gaaccactaa aaactggagt
     5941 ttcgtcgcac gtcaaggctg taaatggaaa cagtagtgga ggtttttcac agttatccca
     6001 gctttctgtg gataacatgg tgtaagatcc tgtttatttt cagtgaccag atttggaaaa
     6061 cctattgcag tgctgcgcaa ctcgattatc ggcagcctaa aaagcaatat aaatcagaga
     6121 attgaacaac acatgtggaa aaagttacac tgcgaatatt cggtacataa ttgctgtttg
     6181 ttttttaacc aaggtatcat gacatgtccc aacctcgccc actgctctct cctcccgaaa
     6241 ctgaagaaca gttgttagcg caagcacagc aactttctgg ttatacattg ggagaactgg
     6301 cggcacttgc cgggctggtt acgccagaga atttaaaacg cgataagggc tggattggcg
     6361 tgttactgga gatctggcta ggtgccagcg cagggagtaa acctgagcaa gattttgctg
     6421 ctctgggcgt ggaacttaaa actatccctg tggatagtct tggtcgtccg ctggaaacaa
     6481 cattcgtttg tgttgccccg ttaacgggta atagcggggt gacctgggaa accagccacg
     6541 tgcgccacaa gctcaaacgc gtactgtgga taccggttga aggcgagcgc agcatcccgc
     6601 tggcgcagcg tcgcgtagga tccccgttgc tgtggacccc gaatgaagag gaagaccgac
     6661 agctacgcga agactgggaa gaattaatgg atatgattgt tctcggtcag gttgagcgga
     6721 tcaccgctcg tcacggggaa tatttacaga taagaccgaa agcagcgaat gcgaaagcgc
     6781 ttaccgaagc cattggtgcc cggggcgaac ggattctgac gctgccacgc ggcttttatt
     6841 tgaagaagaa tttcaccagt gcactactgg cccgtcattt tctgatccag tagccatcgc
     6901 tttgacctgc cgctttccgg gcatataatt accgcttcat ttttttggca gggcttttta
     6961 gatgttattt gcatggataa ccgatcctaa cgcctggctt gcgctcggta cgctgacgct
     7021 gctggagatc gttcttggga tcgacaatat tattttcctt tctctggtgg tggcaaagct
     7081 tcccacagca caacgtgctc atgcgcgccg tctggggttg gcgggagcca tggttatgcg
     7141 tctggcgctg ctggcatcaa tcgcctgggt tacgcgcctg acaaatccgc tttttacaat
     7201 attcagtcag gaaatttctg cccgtgattt gattctgctt ctgggtggct tgttccttat
     7261 ctggaaagcc agcaaggaaa tccacgaatc cattgaaggt gaagaagaag ggctgaaaac
     7321 acgcgtttca tcattcctcg gcgctatcgt gcagattatg ctgctggata ttatctttag
     7381 cctcgactcg gtgattacgg ctgtgggtct gtcagatcac ctgtttatta tgatggcagc
     7441 cgtggtgatt gccgtaggcg tgatgatgtt cgccgcgcgc tcgattggtg attttgtcga
     7501 acgccatcct tcggtaaaaa tgctggcgct ctctttcctg attctggtgg gctttaccct
     7561 gattctggaa agtttcgaca tccacgtacc gaaaggttac atctacttcg cgatgttctt
     7621 ctctattgcg gttgaaagcc tcaacttgat tcgcaacaaa aagaatccgc tctgatactc
     7681 cgtacgctct cctgcgggag ggcgtactca ctccttttac cctcacagtt taagattttc
     7741 ctgctttcaa gatatataac gtcgttttat aaacagacta ttatcatagg taggccaaat
     7801 gaggataacc ggatgaaaaa atgggcagta ataatttctg cagtcggact ggcgtttgct
     7861 gtttccgggt gttccagtga ttacgtcatg gcgaccaaag atggccgtat gattttgacc
     7921 gatggaaaac ctgaaattga tgatgatacc gggctggtga gttatcacga tcagcaaggt
     7981 aacgcgatgc aaattaaccg tgatgatgtt tcgcaaatta ttgaacgtta acaaataagg
     8041 tcagcatccg gctggcctta agatttttct ctttcccttt tcccttccct ctgccatttt
     8101 tatattcctt atgtcgtgat tataaaaagg aaacggctat gcaatatcac cgtatacccc
     8161 acagttcgct ggaagtcagc acgctggggc ttggcacgat gacgtttggt gaacagaaca
     8221 gcgaagccga cgcccacgca caactcgact atgccgtcgc tcaaggcatt aaccttatcg
     8281 acgttgccga aatgtaccca gtacctccgc gccccgaaac gcaagggtta accgaaacct
     8341 acgtcggcaa ctggctggcg aaacatggca gccgcgaaaa gttaattatc gcctccaaag
     8401 tgagcggacc gtcgcgcaat aatgacaagg gcatccgccc ggatcaggcg ctggatcgga
     8461 agaatatccg cgaagcgctg catgacagcc tcaagcgcct acagactgat tacctcgatc
     8521 tttatcaggt gcactggccg cagcgcccga ccaactgctt cggcaaactc ggttatagct
     8581 ggacggattc tgcgcctgcg gtttcgctgc tggatacgct ggacgcactg gcagagtacc
     8641 aacgcgcggg aaaaattcgt tatatcggcg tgtcgaacga aactgcattt ggcgtaatgc
     8701 gctacctgca tctggcggac aaacacgatc tgccgagtat tgtcaccatt cagaaccctt
     8761 acagtctgtt aaaccgcagt tttgaagtag gtctggcaga agtcagccag tatgaagggg
     8821 tcgaactgct ggcctattca tgcctgggtt tcggcacgct gaccgggaaa tatctcaatg
     8881 gtgcaaaaac cgctggcgca cgtaatacgc tctttagtcg gttcacccgc tatagcggtg
     8941 agcaaacgca aaaagccgtc gcggcgtatg ttgatatcgc cagacgtcat ggcctggacc
     9001 ctgctcagat ggcgctcgcg tttgtacgcc gtcaaccgtt tgttgccagc actctgctgg
     9061 gcgcaaccac gatggatcag ctgaaaacta acatcgaaag tttgcatctg gagttaagcg
     9121 aagacgtatt agctgaaatt gaagcggtgc atcaggttta tacttatccg gcaccataag
     9181 attaacgccg gttaaaaccg gcgttaaata ttaatgacgg cgctgccaga tccacagcgc
     9241 cgttattgcc agcgcaaaca gcgcaccgaa gccaatgcca atgggcacga ccgggatgcc
     9301 taccattacc gccagcgagt aaatgcccag catcaacaac atggcgctgt tttcgccaag
     9361 gttttgtact gcaatcgcat tccccgcccc gacgcttttt ttaccccgct cctgtagcaa
     9421 cgcattgagc ggaacgacaa aaaagccccc catcacgcca atcagcatca acaaggcata
     9481 ggctggcagc agctcgtgtt gcagggaaaa aatcagtacc accacgccaa tcaaaatccc
     9541 ggctggcata cagcgtgaca cggtttccag cgtaactaac ttcgctgccg cacctgcgcc
     9601 aaccacgata ccaatcgcta ccatcgcgtt gagataggtg ggcgtagagt tatcggtaat
     9661 gcccagcgcc accggtaccc acagcaccaa caggaaacgc agcgtgacac ccgctcccca
     9721 gaataaactg gtgcccacca gcgaaaaacg cgtttcacca ttgcgccata gcgaggtgca
     9781 ggcattcagg aaactgcggg tcatgttgat gagattccaa gactgccccg gacgcgccgc
     9841 cgccagtttg ggaatgtaga tattggcaac gaccgcacca ccgtaggcca gtgcgcatgc
     9901 ggccagggcg acgaggacat gccagtcagc cagcacacca ccggctacgg aaccgagcaa
     9961 aatcgccgct atggtagaag cttccattaa accgttagct ttcactaact tactacccgt
    10021 ggttaattcg ccgagaatac cgtatttcgc cggtgaatag gctgcagcac caacccccac
    10081 cagcgtatag ccgagaaacg gattgatacc aaagcagata ctggctgcgc ccagcagctt
    10141 caggccgttg gcaaacatca tcacccggcc tttggcgaag ctatccgcca cctgcccgac
    10201 aaacggcgca aaaagaatgt aagcacctac aaacaccatt tgcaggatgg gctggctcca
    10261 ctccggatag aactgcgctt tcagtaacgc cagagtggca aacagtaggg cattatcgcc
    10321 aaacgcagag agaaactgcg ccacgataac cgctttcatc cccttcgacc acaacgaagt
    10381 gttagtgtgc actgactcac tcatcgtgtt gttccgcttc gtctacccag cttttcaacg
    10441 tgacaaagtc aggtttgccg ctgccaagta atggcatctg tttcagatag cgaatatcgc
    10501 gcggtacagc aagctccggc acgccgtgct cgcgggcata ctgttgcaac ttatcgagcg
    10561 tcagttcgtt atctgtggtg aaaagcacca gtgcctcgcc tttgctggca tcgctcttaa
    10621 tcgcagtggc atggacttta tctggcgaaa caccaagtgc cagttgttcc accatttcca
    10681 gcgacaccat ttcgcctgca attttggcaa agcgttttgc gcggccctga atctgcacaa
    10741 agccctgctc gtcaaaacgc acaatatcgc cagtgtcata ccagccgcgc tccatttcgc
    10801 cgcgaacatt ctcggcggtg ggcacttcca gtacacctgg cttctccacc cgcagatagc
    10861 cgttcattat gttcggccct ttcagttgca ggcgtccgcc ctcttcgata ccaggaaccg
    10921 acaacaggcg cgcatccatt cctggtagaa tacgccctac cgtaccgggt ttcgccgcca
    10981 tcggtacgtt gatagaaacg acaggcgcgc actcggttac accatagcct tcaaggatgc
    11041 gcaggccaaa tttatcctgc caaagctgtt tggtactttc ttgtagtttt tctgcgcctg
    11101 ccaccacata gcgtagacga tagaagtcat acgggttggc gaaacgcgcg tagtgaccga
    11161 ggaaagtcga ggtaccgaac aacacggtgc aactgcggtc atacaccaac tccggcacaa
    11221 tgcggtaatg cagcgggctt ggataaagga acacttctgc acctgtaagc agtggcgtaa
    11281 acaggcctac cgtcagccca aaggagtgaa acagcggtaa tgccgacata aagcgatcgt
    11341 tggtggtgaa gtcggcaatc gttttaatct gctcgacgtt cgccagaatg cttttatggc
    11401 tatggacgac gccttttgga tggccttcag aaccggaggt aaagaggatc agcgcctctt
    11461 cttccggctg ctgttttacc tgcgccaggc gcggcatcag caaatgagcg aagatccata
    11521 ctttgtcggc agtggtgaca tctgctttta aatctaccag atagacccag cgcacctgag
    11581 taagttgctc cggcagatgc cagagtttgc ctttatcgag aaactggcgg gaagtgaaga
    11641 tggttttgat ttcagccgcc gtaatagcac tggtcagccc ttttacccca gcagtgtagt
    11701 tcatcattgc gggaatgcgg cgacgcgcga tggccccaaa atcactgccg cactgatgcc
    11761 tgcattgggc agcattaagc cgatgcgttc gccttcaaca ctgtattttt caaggatgcg
    11821 tccaacaaac agcgttttcg taagcaattt gcgataggag tctggggtaa agttgacgtc
    11881 ttcgacacat ttcttcccgg ctccgaagcg gtacattgca ctcagtaaag attcgtacag
    11941 agtttcacgc gggcgcaccg ccatacgcgc ttccatcatt atttgatgca gcatttcgcc
    12001 agcgattttg cgacggtcac gggcacgcga cgcatccggc atcgccacct gcgttggagg
    12061 caaaatatgc agagtaattt gcgggaacaa gcgacgttta accagacctt tcaggcggct
    12121 gaagtgcgta agttccgccc cttcaatacg cacaggaata accgttgcac cagacttcgc
    12181 cgcgacaaaa cccgcgccat cgtagatttt catcagcgag cctgtagtgg tgatgcgtcc
    12241 ttcagggaaa atcaccactg gtcggccctg ttccaccaga cgtaccagat gtttaatagc
    12301 cataggttgc gtcgggtcga gaggaacaaa gtcgataaat gatttcagcc aacgcatata
    12361 ccactgttgg cttattgagg tgtaaacggc aaacactgga cgcacaggta aaaacagtcc
    12421 aagcaaaatg ccatcaataa aagagacgtg attaggcgta attagaacgc gctcgccctt
    12481 cagtgcctgg gtgtcacccg taacgcgaac gcgatacaaa acacggcaca aatttcgaaa
    12541 aaagctaaaa agcattccaa ctccctttgc tctgattcag taaaagcgaa tggagggaga
    12601 ttacacgaga taaagaacgc gagcgacagt aaattaggtg cgaaaaaaaa cctgcgcatc
    12661 cgcgcaggtt ggtgcaagag acagggtacg aagagcgtac cgaataatct caccaatcaa
    12721 tacctctggg atcaccactt tagcaacctg aagccaaacg ccaccagcgg tcaatcgcaa
    12781 cagcgtttcg caaagtgtaa ccaaaggttt gcaatctctt tttttgtctt acttatttgc
    12841 gtttggctca cattcccacg atgaaaacac gccactcctt gaaccaacgg gcgttttccg
    12901 taacactgaa agaatgtaag cgtttaccca ctaaggtatt ttcatggcga ccataaagga
    12961 tgtagcccga ctggcaggcg tttcagtcgc caccgtttcc cgcgtcatta ataattcacc
    13021 caaagccagc gaagcttccc ggctggctgt gcatagtgca atggagtctc ttagctatca
    13081 cccgaacgcc aacgcccgtg cgctggcgca gcagaccact gaaacggtcg gtctgatcgt
    13141 tggtgatgtt tccgatccgt ttttcggtgc aatggtgaaa gcggtcgaac aggtggctta
    13201 tcacaccggt aatttcttat tgattggcaa cggttaccac aacgaacaaa aagagcgtca
    13261 ggctattgag caactgatcc gccatcgctg tgctgcgttg gtcgtccatg ccaaaatgat
    13321 cccggatgcc gatttagcct cattaatgaa acaaatgccc ggtatggtgc tgatcaaccg
    13381 tatcctgcct ggctttgaaa accgttgtat tgctcttgac gatcgttacg gtgcctggct
    13441 ggcaacgcgt catttaattc agcaaggtca tacccgcatt ggttatctgt gctctaacca
    13501 ctctatttct gacgccgaag atcgtctgca agggtattac gatgcccttg ctgaaagtgg
    13561 tattgcggcc aatgaccggc tggtgacatt tggcgaacca gacgaaagcg gcggcgaaca
    13621 ggcaatgacc gagcttttgg gacgaggaag aaatttcact gcggtagcct gttataacga
    13681 ttcaatggcg gcgggtgcga tgggcgttct caatgataat ggtattgatg taccgggtga
    13741 gatttcgtta attggctttg atgatgtgct ggtgtcacgc tatgtgcgtc cgcgcctgac
    13801 caccgtgcgt tacccaatcg tgacgatggc gacccaggct gccgaactgg ctttggcgct
    13861 ggcggataat cgccctctcc cggaaatcac taatgtcttt agtccgacgc tggtacgtcg
    13921 tcattcagtg tcaactccgt cgctggaggc aagtcatcat gcaaccagcg actaaccgca
    13981 gttaaagcaa ttccagcgcc agtaattctt cgatggtctg gcgacggcga atcaaccgcg
    14041 cctgaccatt atcaaacaga acttctggta acagcgaacg gctattgtag ttggatgaca
    14101 ttgatgcgcc atatgcccct gtatcatgca gtaccagata atcacctgcc ttcacttccg
    14161 gcaaggcgcg ggtttcaaca tttccccctt cctgctgggt aaagacatcg cccgattcac
    14221 ataacggtcc ggcgacgacg gtttccaccg ttggcgcgtg ttccagagaa cgaccatcag
    14281 ctgccagggc actgatatgg tggtaactac cgtacattgc cgggcgcatc agatcgttga
    14341 acccggcatc aaccagcaca aagtggcggc tccccatttg tttgacgctc cgcacctgag
    14401 taattaatac gccagactgc gctaccagga agcgacccgg ttcaatttcc agtttcacag
    14461 ggtggcccaa atggcgggcg atttgctcac gcgcggcatt ccacagacca taataatgtt
    14521 cggtatcaac cgcctcttca ccctgttgat aaggaacaga aagcccaccg cccgcagaaa
    14581 tagcctgtaa atcctgaccg aattcgatga cctgacgcac catagcacca cacacctgtt
    14641 ccagatgggc ataatcaacg ccagaaccaa tgtgcatgtg aatgccgacc agctgcagat
    14701 gatgacgttg tatcacgtcc agtgcggcgg gcagatcggt gtaccagata ccgtgcttgc
    14761 tgttttcgcc accggtattg gttttttggc tatgtccgtg accaaacccc ggattaacgc
    14821 gcagccatac ccgatgccct ggcgaaacct ggcccagttg gtcgagcata tcaacagaac
    14881 ccgcattcac cggaatttgc aattcactga cgcgttcaag cgtcgcctga tcgataacat
    14941 ctgccgtaaa aacaatatca tcggggtgcg tttgcggatt gtaacccgcc gccaacgcac
    15001 gctctatttc gcctaacgag acggaatcca ctttcacgcc ctgctcacgc attaagcgca
    15061 aaatatgaat attggaacag gctttctgtg caaagcgcac cacatcaaac tgtttcagcg
    15121 ctgcaatctg ccgacgaata atttgcgcat cgtagaccca caccgggcag ccaaattcag
    15181 cgggcaaacg cagcagattt tcggcggtga gatcggtatc ggtgctgaac agtgaatgtg
    15241 gcataacaaa ctccagataa gtgctttttt atgattacgc cacatcataa aaagaataaa
    15301 aaatatcgat ttatgtcgag tctatgcaaa aatgatatgg attaccggat tgcgagagag
    15361 cgctaatggc cgccgttaac ttacgtcata ttgaaatttt tcatgcggta atgaccgccg
    15421 gaagcctgac tgaggcggca cacctgctac acacctcaca gccaaccgtc agccgcgaac
    15481 ttgcgcgctt tgagaaggtg atcgggctga aattgtttga gcgcgtacgt gggcgattac
    15541 atcctaccgt gcaaggactg cgtctgtttg aagaagtgca acgatcctgg tacggactgg
    15601 atcgaattgt cagcgctgca gaaagtctgc gcgagtttcg ccagggagaa ctgtctattg
    15661 cctgcctgcc ggtcttttcg caatcttttt taccgcagct cctgcaaccc tttctggcac
    15721 gttatcccga tgtcagctta aatatcgtgc cccaggaatc accgctactt gaagagtggc
    15781 tctcggccca gcgtcatgat ttaggactca ctgagacact ccatacgcct gcgggaacag
    15841 aacgtaccga attactctct ttagatgaag tgtgtgtgtt acctccgggt catccgctgg
    15901 cggtaaaaaa ggtattaacg ccggatgatt ttcagggtga gaactacatc agcctttccc
    15961 gtactgacag ctatcgccag ttgctggatc agctatttac tgaacatcag gttaaacgac
    16021 gcatgatcgt agaaacccac agcgccgcgt caatctgcgc aatggtacgg gcgggggtag
    16081 gtgtttcggt ggttaacccg ctcaccgcac tggattatgc ggcaagcggt ttagtggtgc
    16141 ggcggttcag tattgcggtt ccgttcacag tcagcctgat ccgccccctg caccgcccgt
    16201 catcagcgct ggtgcaggcg tttagtgagc atttacaagc ggggttaccg aaactggtca
    16261 cttctcttga cgctattttg tcgtcagcta cgacagcata aaagcgacag catcctcggc
    16321 atggatcgcc gcagtatcaa acacaggcaa aacactgcgc tcttctggca ccagtaaacc
    16381 aatttctgtg cagccaaaaa tgacgccctg tgcgccctgt tctgcaaggc gagcaatcac
    16441 ttgcgcataa taagcgcgtg acgcttcggt aaattgcccc agacacagtt cttcaaaaat
    16501 aatctgatta attttcgctc gttcatccgc ttcaggaata agacaattga tggaaaattg
    16561 ttccgtcagc cgcccgcgat aaaaatcctg ttccatggtg taacgcgtac ccagcagcgc
    16621 cacacgagtc attccggccc cggtaattgc acgtccggtg gcatccgcaa tgtgtaagaa
    16681 aggcagagtg caacgtgact caatggcatc cgccacttta tgcatcgtat tggtacataa
    16741 cacaatacct tctgcgcccg cccgctgtaa gccaagcgcc gcctcagcca gaatgtcccc
    16801 ggttttatcc cattccccgc gacgctggca ctcttctatt tcatgaaaat cgacgctatg
    16861 tagcagcact tgcgcagagt gaagcccacc aagccgctgt ttaatgcctt catttatcaa
    16921 acgatagtaa ggaatggtgg attcccagct cattcctccc agcaaaccaa ttgttttcat
    16981 tcctctctcc tgatgtgtgt taccgcaaca aggctgaata aggaacgaga caaacgcctc
    17041 aacggccaag tgcccaatct ctattaacga aaaaagggcc ggatgtacag cacatccggc
    17101 ccgtgaaatc agatgccgat atttctcaac ttctcgcctg ccatcagttt gcgttcgata
    17161 tgttccagcg tgacattttt ggtttccgga atgagccaga aagtgatgcc cacaaacgca
    17221 atgttcagcg cagtgtagag ccagaacgta ccggcagcgc caatgctgtc gagcagcgtc
    17281 aggaaggtcg ccgataatca tattcgacac ccagttcgtg gtggtcgaac aggtaatacc
    17341 gaaatcgcgg catttcagcg gctgaatttc agagcacagg atccacacca ctggcgcggc
    17401 gctcatcgca taaccggcaa tacacatcat cgtcatgcca acagagagcc aggacaatcc
    17461 actggaagcc gtaccgttat caaactgcat caggcaatag cccagcacca gagtgcctaa
    17521 cgccatcacg ctgaaaccaa ttttcagagc cggtttaccc ctgctttatc taccgtaaac
    17581 accgcaataa aggtggcgaa cataaaggtc agccctacga ccagagtcgc aatcatctgt
    17641 tgttctgtgg tcgtaaagcc cgccattttg aagatacgcg gcgcgtagta catgatgatg
    17701 ttcataccgg taaactgctg catcgcctgc aacaacatac cgagaaacac agcacgacgg
    17761 acgttacggt tgatcttaaa cagtgcccaa ccgccctgtt ttaacttcag gctttcacga
    17821 atttcgttga gttcttctcg cgctttttcc gacgtatcgc gcagcatacg caatacttct
    17881 tccgcctcaa tatgacgccc cttttccgcc aaccagcgcg ggctatttgg caggaagacc
    17941 accagaataa tcagcagaac tgctggtaaa gcaagaaccc ccaacattgc gcgccagtta
    18001 ccgctataac tgaacgctgt atcggataaa aacgccagca cgatgccgag tgtgaccatc
    18061 aactggtaca tactgatcat cttaccgcga acgttttcac ttgccatttc agaaagatac
    18121 agaggagcgg tgtaagacgc gatcccgaca gcaatgccca gccccacacg agcggcgatt
    18181 aacatctcta cgctggtcgc aaaagcggac cctatagaac cgagtacaaa caggatggcc
    18241 cccgccatca ggctgtattt acgccccagg cggaacgaca gccaaccatt aaacagcgca
    18301 ccaattgctg cgccgagcat catgctacta accacccatt cctgcaaatg actggtcagc
    18361 acaaagtgat cggtaatgaa cggcaacgct ccggcgatta cgccgatatc aagaccaaat
    18421 aacaatcctg cgaccgcagc agctaccgaa acaaacatat tcatacgccg cgtatcccgc
    18481 aaagaacgtg gcgttaaagc agattccgta ttgatagtaa ccattttttc ctgccagcag
    18541 agagtaagac atagtgaaaa aatacgtgaa caactcacgc aggtgtcagg tcggaaacag
    18601 cataaatatg gattaaattg ctgcgacatg tcgttatgtg atggatattc caattttcaa
    18661 attaagttga attattgaga ttattattaa ccacctaatt ttacagcaga taaaattcat
    18721 aaagttcatt aattgataat taatatggat tatttcataa ccatgatatg gattatgatg
    18781 atctacaggt ataaaaaacc ctgccatgcg gcagggtcat aaaagtaaga agaatgaatt
    18841 aacgcgccag ccaaccgcca tccacggcaa tggtataacc attcacataa tctgaagcgc
    18901 tggaggcaag gaacactatc ggccccatca ggtcactcgg cagtccccaa cgaccagctg
    18961 gaatgcggtc gagaatttcc gcgctacgtt gttcatctgc ccgtagttgt tgagtattgt
    19021 tggtcgccat gtaacccgga gctatcgcat taacattaat gttgtgttta gcccattcgt
    19081 tcgccatcaa tcgcgtcaca cccatcacgc cgctttttga tgcggtataa gaaggcacac
    19141 ggatcccgcc ctggaaggag agcattgacg cgatattgat aatcttgccg ccattgcctt
    19201 gcgcgataaa gtgtttcgcc gctgcctgag acatgaagaa tacgctcttg atattcaggt
    19261 tcatgacatc gtcccagtct ttttcgctga actcgagagc gtcttcgcgg cgaatcaatc
    19321 cggcgttatt caccaggata tcaatatgac caaattcagc taccgcgcga tccagcagtc
    19381 caggaatgcc atcaatcttt cgcagatcgg cggtcaggct taaaaaacga cgccccagcg
    19441 ccgtgacctg ctcgatggtt tcagtcggtt caacgatgtt aatgccaaca atgtcacagc
    19501 ccgcttgcgc cagccccaac gccatcccct ggcccagccc agtatcacaa ccagtgacga
    19561 ccgcaacttt accttcgaga gaaaatgcac ttaaaatcat aacaatacct tattcgttta
    19621 tgcccacaac tagcgcaaat ctttaacggc cacatggtcc atatcatcaa agacctggtt
    19681 ttcaccaacc atgccccaga taaaggtata agctttggtt ccgacgccgg aatggatcga
    19741 ccagctcggg gagatcaccg cctgctcgtt atgcatcaca atgtgacgcg tttcttgcgg
    19801 ttgccccatc atgtggaaaa cgcaggcgtc atcatccata ttgaaataga aataaacttc
    19861 catccggcgc tcgtgggtgt gacacggcat ggtgttccac aagttacctg gagccagctc
    19921 cgtcagcccc atactcaatt ggcaggtttc cagcacatcc gggacaaaat atttgttaat
    19981 agtgcgacgg ttactggtga ggttatcgcc taacgtgact ggagatactt cgtccggggt
    20041 gacttttttg gtgggatacg tcgtatgcgc gggtgcgcaa ttgtaataaa actttgccgg
    20101 agtggcggta tcaatactgg caaagacaac ttcttttgca cctttaccaa catacagggc
    20161 gtcgcggtga ccgatttcat agcattggcc atcgacagta atcgtaccgg caccgccaat
    20221 attgataaca cctaactcgc gacgttcaag gaaatagctt acgccgagtt gtttaccaac
    20281 ttccccgcca acggaaaccg ttttagttat cggcataatg ccgccaacaa taattcggtc
    20341 aatgtggctg taaaccatgg tgtactcatc ggcgacaaat accttttcaa ccaaaaattc
    20401 attgtgcagc ccttgggtat ccagcgtttt tgcgtgcgca ctgtggatgc tctgtcttac
    20461 gtccacatca acctccgata atcagtgaag cgtgaaaata gaaaacgata gtgaaacact
    20521 gtttcgtttt ctctataaag ggatgatatg cccatgggct agggttttca attaaaaata
    20581 aaacaatgtt ttatttttaa gttaaggatt aaaaaaagtg cagtgtcgat cacgaatatg
    20641 tcatgagaat ggaaaaatga gggagcccag aaaatgtatg tcgac
//
LOCUS       DADLFA010000060        20657 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000060 DADLFA010000000
VERSION     DADLFA010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20657
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.060"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..876
                     /locus_tag="JQH65_001862"
     CDS             <1..876
                     /locus_tag="JQH65_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY6139296.1"
                     /translation="DQTSWQLSHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQ
                     YGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKG
                     FSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTN
                     IINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAED
                     GGFTLEINGSEKELYLLSGQQFLKCPLSVEKKRASIRYSGDVTCSVVTYSQLPELKQP
                     KLRGNVQTAQREVAP"
     gene            873..1238
                     /locus_tag="JQH65_001863"
     CDS             873..1238
                     /locus_tag="JQH65_001863"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HAY6139297.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGQRASDES"
     gene            1330..1962
                     /locus_tag="JQH65_001864"
     CDS             1330..1962
                     /locus_tag="JQH65_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HAY6139298.1"
                     /translation="MKNLPIGGVWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFP
                     EFAHATPRVQLDLYPTGSVNGSNYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAA
                     GRRDGDFSIYRQGGTSTDEGERIDYRVKMYNPETGGQIDVRNNENMVWNSINLKSVRP
                     VVLPGIRYAVMCVPTPLALAVDKFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(2050..3348)
                     /locus_tag="JQH65_001865"
     CDS             complement(2050..3348)
                     /locus_tag="JQH65_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY6139299.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSRGGLA
                     IARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFSLVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQESHNDPAPQH"
     gene            3831..5243
                     /gene="uxaC"
                     /locus_tag="JQH65_001866"
     CDS             3831..5243
                     /gene="uxaC"
                     /locus_tag="JQH65_001866"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAY6139300.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLLSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            5258..6745
                     /locus_tag="JQH65_001867"
                     /pseudo
     CDS             5258..6745
                     /locus_tag="JQH65_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
     gene            6828..7379
                     /locus_tag="JQH65_001868"
     CDS             6828..7379
                     /locus_tag="JQH65_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HAY6139301.1"
                     /translation="MTAYWLAQGVGFIAFLIGITTFFNRDDRRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTRALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(7384..8628)
                     /gene="sstT"
                     /locus_tag="JQH65_001869"
     CDS             complement(7384..8628)
                     /gene="sstT"
                     /locus_tag="JQH65_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAY6139302.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDLSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(9244..10209)
                     /gene="alx"
                     /locus_tag="JQH65_001870"
     CDS             complement(9244..10209)
                     /gene="alx"
                     /locus_tag="JQH65_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HAY6139303.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINARVNYRHDKQRGE"
     gene            complement(10493..11497)
                     /locus_tag="JQH65_001871"
     CDS             complement(10493..11497)
                     /locus_tag="JQH65_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAY6139304.1"
                     /translation="MEYPRVMIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQ
                     AQHFANDFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPL
                     ASNLAEVDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSS
                     RYQRYLDGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAH
                     GVVVMDYGDFSVTLLHSKVSDSVLESEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDL
                     TQPQHINTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL
                     "
     gene            complement(11558..12241)
                     /locus_tag="JQH65_001872"
     CDS             complement(11558..12241)
                     /locus_tag="JQH65_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HAY6139305.1"
                     /translation="MQPRLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKNYDE
                     ASGMQKALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYSIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHSQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            complement(12318..12821)
                     /locus_tag="JQH65_001873"
     CDS             complement(12318..12821)
                     /locus_tag="JQH65_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAY6139306.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            12906..14042
                     /gene="rlmG"
                     /locus_tag="JQH65_001874"
     CDS             12906..14042
                     /gene="rlmG"
                     /locus_tag="JQH65_001874"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HAY6139307.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQSWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            14327..14641
                     /locus_tag="JQH65_001875"
     CDS             14327..14641
                     /locus_tag="JQH65_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HAY6139308.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNMIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            14638..15054
                     /gene="higA"
                     /locus_tag="JQH65_001876"
     CDS             14638..15054
                     /gene="higA"
                     /locus_tag="JQH65_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HAY6139309.1"
                     /translation="MIAIADILQAGEKLTAVVPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLADLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(15099..17117)
                     /locus_tag="JQH65_001877"
     CDS             complement(15099..17117)
                     /locus_tag="JQH65_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HAY6139310.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLHTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSESGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELNARRAIAQGTRLALEI"
     gene            complement(17543..19894)
                     /gene="ygjK"
                     /locus_tag="JQH65_001878"
                     /pseudo
     CDS             complement(17543..19894)
                     /gene="ygjK"
                     /locus_tag="JQH65_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
     gene            complement(19911..>20657)
                     /gene="ygjJ"
                     /locus_tag="JQH65_001879"
     CDS             complement(19911..>20657)
                     /gene="ygjJ"
                     /locus_tag="JQH65_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HAY6139311.1"
                     /translation="PDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWG
                     DFTPDLGYVIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDD
                     KYTYPAMMNGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADG
                     LDVPGSESGMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGR
                     FATAFDSRFYMKNASHDPSDQIFLMQYFYW"
BASE COUNT         5035 a         5410 c         5431 g         4781 t
ORIGIN      
        1 gatcaaacgt catggcagtt atcgcatacc ttttataatg attcattcag ccataaagaa
       61 cttggcgtaa ccgtcggagg cctgaacacc gataccataa acagcgcggt taacgggcgt
      121 tgggatgggc aatacggaaa tgtctacgct accgtatctg atagttatga ccgtaagaat
      181 catgatcatc tttcggcctt tacgggcact tatagctcta cgctggctgt cagtcgctat
      241 ggcgttaatt tgggagccag tggtacagac gatttgctgg gtgcggtatt ggtggatgtg
      301 aaaggcttct ctgaacagga tgaagagagt caggatctgc aactcgaagc gcgggtggca
      361 ggcagccgaa ctttgcagct tggtcaaagc gacagtgtgt tgttccctta tcctggattt
      421 cagtctggtt ttgttgaggt taacgacagt agccagggca atcagcaggg gacaacaaac
      481 atcattaacg gtgcagggaa tcgtgaatta atgttgttgc ctggcaagct gcgctatcgc
      541 gaagtgtctg ccagctttaa ctacaactat atcggtcgtt tattactgcc ggcagcggtg
      601 aaaaaattcc cgattgtggg gctgaacagc gccatgttac tggtagctga agatggcgga
      661 tttacacttg aaattaacgg cagcgaaaaa gagctctatc tgctttccgg gcagcaattc
      721 cttaagtgtc cactgagtgt tgaaaagaaa cgcgccagca ttcgttacag cggagatgtt
      781 acttgtagtg tggtgactta ttcacaatta ccggagttga aacagcctaa attacgtgga
      841 aacgttcaga cggcgcaaag ggaggttgca ccatgagaaa tcgattgatt gcggcgatat
      901 tgggcttgtt tggcacgctc actggcgttc aggcagctcc tgacgtgacc agtgaaatta
      961 cgtatgattt ggcatctggt agagcggatt attacttctg gaaggatgaa gcgtcggcag
     1021 gaaataatgg atatatgtgg tatgaatgtt cgtatcctga cctccaacaa acctgtacag
     1081 ctaatggaaa tatatcgaca gtacaaatct atttaactga acaacgcagt gggatgcgtt
     1141 ggccggtaaa actcaaagga tttaaaacag ccattgtaag tagcgatgaa gcgccgctag
     1201 gatgcaaggg ggggcaaagg gcttcagacg aatcttaagg attctaatag atcttcatgt
     1261 acagaagatg gtcaacatta ttatatatac gatacaaagt ttcttacgct ctaccttgag
     1321 cagacagaga tgaagaattt gccgattggt ggcgtctgga aggggaaagt taaattacat
     1381 tcgaacagcc cggcccagga ctatttcgca aatattaccc tgaatacgct cgaccccaac
     1441 catattgacg tgttcttccc ggagttcgcc cacgccacgc cgagggtgca gttagacttg
     1501 tatccaacag gaagcgttaa cggcagcaac tacgcgcaag atctgaccat gttggacatg
     1561 tgcctgtacg atggttttaa cggtaatgcc atcagttatg aaatcatgct caaagatgaa
     1621 gggcgaccag ctgcagggcg cagagacggt gacttctcta tctatcgtca gggaggaacc
     1681 tctaccgacg agggagaacg cattgattac cgggtcaaaa tgtacaaccc ggaaaccggt
     1741 gggcaaattg atgtgcgcaa taatgaaaat atggtctgga acagcattaa cctgaaaagt
     1801 gtgcgtccgg tggtactgcc cggtattcgc tatgccgtga tgtgtgtgcc gacacccttg
     1861 gctctggcag tcgataaatt cagcgtgatg gataaacagg ccgggtacta catgggcaaa
     1921 ttgtcagtaa tctttacgcc ttccttgcca accatcaatt aaccctgcaa agcggacaaa
     1981 cgcttttttc tatataaacg acgaagccgc ctgataaggc ggcttcgtcg ttataactta
     2041 cgttaaacgt taatgttgcg gtgcgggatc gttatgtgat tcctgcgcta cctcaatcgc
     2101 cggtttgttc tgcaacacgg tccagataac cagcgcacct aacaggtcga acactgccag
     2161 tactgcgaac agcgggctga agccgatggt gtcagccagt gcaccgacaa ccagcgaaaa
     2221 cagcgtactt gccagccatg cggacatccc ggttaaaccg ttcgccgtcg ccacttcgtt
     2281 acgaccgaac acgtcagaag agagcgtaat cagcgcgcca gacagtgcct ggtgagcaaa
     2341 accaccgata cacagcagca taattgcgac atacgggttg gtgaacaggc cgatcatacc
     2401 cgggccaatc atcagcactg cacccagcgt tacgaccatc ttacgggaaa cgatcaggtt
     2461 cacaccaaac caacgctgga acagcggcgg caggtaacca ccgaggatac aaccgaggtc
     2521 agcaaacagc atcggcatcc aggcgaacat cgcgatctct ttcaggttaa agccgtaaac
     2581 tttaaacatg aacagcggga tccacgcgtt aaaagtaccc caggctggtt ctgccaggaa
     2641 acgcggcagc gcgatacccc agaactgacg gttacgcagg atctgaccaa cggacatttt
     2701 cttcgccgtg ctcacctggt gctgggcttc ctgaccatta ataatatagt cgcgttcttc
     2761 atcggtcaga tgcttctggt cgcgcggatg tttatagaaa atcagccatg ccatcgccca
     2821 gataaagctc aatgcaccgg agatgataaa tgccatctgc cagctgtgca ttacgattgc
     2881 ccataccacc agcggcggcg caatcatcgc accaatcgaa gaacctacgt taaagtaacc
     2941 taccgcgatg gaacgctctt tcgccgggaa ccattcggag ctggctttca gacccgccgg
     3001 gatcatcgct gcttccgcgg caccgactgc accacgagca atcgccaggc cacccctgct
     3061 accagccagc gcggttgcac cacagaatac ggcccacagt acagcaaaca ttgcataacc
     3121 gattttcgta cccagcacat ccagtacata gcccgctacc ggttgcatga ccgtataagc
     3181 agcagaatag gctgcgatga tataggaata ctgttgggtg gagatgttta actcttccat
     3241 cagagttggc gcagctgccg ccacagtgtt acgcgtcagg taaccaagca cggtgccaag
     3301 cgtcaccagt gcgatcatat accaacgtaa ccctttaatt ttacgcatgt aaaacctcat
     3361 cgttttgtta tatccgcagg aagcgcggcc atcatcccgc cagaggctga agatgttagt
     3421 tacgaccggg taaccgaaaa aaaaccggca cgccccgaac gttgccatga aagtttcgtg
     3481 ctaattcggc ttccgtaccg gttaacccga ttcatctaac aaatgaatga atcggcaatt
     3541 tgcattccgt cgacttatga tttgcgacgg cagaaagatg acttgtcata caactttaaa
     3601 aggtgagagc catcataaat gtgcgaatat ttgtagggac attacctgac gacagcgagg
     3661 ccagtactgg cgcggcctgc agcgagattt accactttga gagtaatttt tttaactacg
     3721 tttattgatc taactcacga aaatatcttc ggactctgga aattggtgtg ataactttgt
     3781 cagcatcgca ccataagcaa gctagctcac tcgttgagag gaagacgaaa atgactccgt
     3841 ttatgactga agatttcctg ttagataccg aatttgcccg ccgtctgtat cacgactacg
     3901 caaaagacca gccgattttc gattaccatt gccatttgcc gccgcagcag attgcggaag
     3961 actatcgttt taaaaacctg tatgacatct ggctgaaagg cgatcactac aaatggcgcg
     4021 ctatgcgtac caacggtgtg gccgagcgtc tgtgtaccgg tgatgcgtct gaccgtgaaa
     4081 aatttgacgc ctgggcggcg actgttccgc acactatcgg caacccgtta taccactgga
     4141 cgcacctcga actgcgtcgt ccgtttggta tcactggcaa attgctttct ccgtcaactg
     4201 ccgatgaaat ctggaacgaa tgtaacgaac tgctggcgca ggataacttc tccgcgcgcg
     4261 gcatcatgca gcagatgaac gtgaaaatgg tcggcaccac cgatgacccg atcgattctc
     4321 tggagcatca cgcagagatc gccaaagacg gctctttcac catcaaagtg ctgctgagct
     4381 ggcgtccgga caaagccttc aacatcgaac aggcgacctt taacgactac atggcgaagc
     4441 tgggcgaagt ttccgatacc gacattcgcc gctttgctga cctgcaaact gccttgacca
     4501 aacgtctgga tcacttcgcc gctcacggct gtaaagtgtc tgaccatgcg ctggacgtgg
     4561 tgatgtttgc tgaagcgaac gaagcggaac tggacagcat tctggcgcgc cgtctggctg
     4621 gcgaaaccct gagcgagcac gaagtggcac agttcaaaac tgcggtactg gtgttccttg
     4681 gtgccgaata tgcacgtcgc ggctgggtac agcagtacca cattggcgcg ctgcgtaata
     4741 acaacctgcg tcagttcaaa ctgctggggc cggatgtagg ctttgactcc atcaacgacc
     4801 gtccgatggc agaagagttg tctaagctgc tgagcaagca gaacgaagaa aacctgctgc
     4861 cgaaaaccat tctgtactgc ctgaacccgc gcgataacga agtgttgggc accatgatcg
     4921 gtaacttcca gggcgaaggt atgccgggca aaatgcagtt cggttccggc tggtggttta
     4981 acgatcagaa agacggtatg gaacgtcaga tgacccaact ggcgcagctc ggtttgctga
     5041 gccgctttgt cggtatgctg actgacagcc gtagcttcct gtcatacacc cgtcacgaat
     5101 acttccgccg cattctgtgc cagatgatcg gtcgctgggt ggaagcgggc gaagcaccgg
     5161 cggacatcaa cctgctgggc gagatggtga aaaatatttg ctttaacaat gcgcgtgact
     5221 acttcgccat tgaactgaac taaggtctgg ggttgatatg caatacatca agatccatgc
     5281 gctggataac gtcgcggtcg ctttagcgga tttggctgaa ggcacagaag tcagtgtcga
     5341 taaccagact gttacgctgc gccaggatgt tgctcgtgga cataaatttg cgttaacgga
     5401 tatcgcaaaa ggggccaatg tcatcaaata tggcctgccg attggttatg cattggcgga
     5461 tattgcggcg ggggaacacg tccacgccca caatacgcgc acgaatctga gcgatctgga
     5521 tcagtatcgc tatcaacctg attttcagga tctgcctgcg caagcggcag atcgtgaagt
     5581 gcagatctat cgtcgcgcta acggcgatgt cggggtgcgt aacgagctgt ggatcctgcc
     5641 aaccgtgggc tgtgtcaacg gcatcgcgcg gcagatccag aaccgttttc tgaaagagac
     5701 caacaacgct gaaggtaccg acggcgtgtt cctcttcagc cacacctacg gctgctcaca
     5761 gctgggcgac gatcacatta atacccgcac catgctgcaa aacatggtgc gccacccgaa
     5821 cgcaggcgca gtgctggtga ttggtctggg ctgtgaaaac aactaggttg ccgcattccg
     5881 tgaaacgctg ggcgatatcg atcctgaacg cgttcatttc atgatctgcc aacagcagga
     5941 tgatgagatc gaagccggga tcgagcattt gcatcagctg tataacgtga tgcgcaacga
     6001 taaacgcgag ccaggcaaac tcagcgaact gaagtttggt ctggagtgcg gtggttctga
     6061 cggtctttct ggtattactg ctaacccgat gctggggcgt ttctctgact acgtgattgc
     6121 taacggcggt actaccgtac tgaccgaagt gccggagatg tttggcgcag agcagttgct
     6181 gatggaccat tgccgcgacg aagcaacgtt tgaaaaactg gtcaccatgg tcaacgactt
     6241 taaacagtac tttattgccc atgatcagcc gatctacgaa aacccatcac cggggaacaa
     6301 agcgggcggt atcaccacgc tggaagacaa atcacttggc tgtacccaga aagcgggttc
     6361 cagtgtcgtg gttgacgtgc tgcgttacgg cgagcgtctg aaaacgccag ggctgaactt
     6421 gttaagtgcg ccgggtaacg atgccgtagc gaccagcgcc ctggcgggtg cgggctgcca
     6481 tatggtgctg ttcagtactg gtcgcggcac gccatacggt ggatttgtgc cgacggtgaa
     6541 aatcgccacc aacagtgaac tggcggcgaa gaaaaaacac tggctcgact ttgacgcggg
     6601 tcagctgatc cacggtaaag cgatgccgca gttgctggaa gaatttatcg acaccatcgt
     6661 tgagtttgcc aacggtaaac aaacctgcaa cgagcgtaac gacttccgcg aactggcgat
     6721 cttcaaaagc ggcgtaacgc tataaagtcg aaatgagttg ttaataaaaa acggcgtttc
     6781 ataatgtgaa gcgccgtttg cttttccata cctgataagg atttatcatg accgcgtatt
     6841 ggctggccca gggcgtgggt ttcatcgcct ttctgattgg tatcacaaca tttttcaatc
     6901 gtgacgaccg tcgcttcaaa aagcagcttt cggtctatag cgccgttatt ggcgtacatt
     6961 tttttcttct gggcacctat cccgctggtg ccagtgccat ccttaatgcc attcgtacat
     7021 tgattacctt acgcacgcgc agcttatggg taatggcgat ttttattgtg ctgactggcg
     7081 gaattggcct cgcgaagttc catcatcctg tcgaactatt gccggttatc ggtacgattg
     7141 tcagtaccag ggcgctgttc cgctgtaaag ggctcaccat gcgctgcgtg atgtggtttt
     7201 caacgtgttg ctgggtgatt cacaacttct gggcggggtc gataggcggc acaatgattg
     7261 agggtagttt tctgcttatg aatggcttga atatcattcg tttctggcgg atgcagaaaa
     7321 ggggaattga tccgtttaaa gtagagaaaa cccctcccgc cgtagacgaa aggggttaaa
     7381 caattaatta cgcagggcgc tatttgccag acgatcgtct tccgcctggc aagctgccgc
     7441 agtgaacagc acgtcagttg aagagttcag cgcggtttcg caagagtcct gcaatacgcc
     7501 gatgataaag ccgacggcaa ccacctgcat ggcgatatcg ttcgaaatac cgaacatatt
     7561 acaggccagc gggatcagca gcagagaacc ccccgccacg ccggatgcgc cacaggcaca
     7621 cagagaagcc accacgctca acagcagcgc cgtgggcaga tcgaccggga tacccagcgt
     7681 attaaccgca gccagcgtca acacggtaat ggtgattgct gcgcccgcca tattgatggt
     7741 ggcacccagc ggaatagaaa cggaataggt atcgcgatcc agattcagct tttcacacag
     7801 tgccatattc accggaatgt tcgccgcaga gctgcgggtg aagaaggcat acacgccgct
     7861 ttcgcgcagg cacagcagca ccagcgggaa cgggttacga cgaattttcc accacaccag
     7921 caatgggtta accaccagcg ccaccagtaa catacagcca accagcacga ccagcagttg
     7981 cgcgtagccc cacagtgtgg agaaaccggt ggttgccagg gtagaagaaa ccagaccaaa
     8041 aataccaatc ggtgcgaagc gaatgaccag tttcaccata aaggtgacgg cattcgacag
     8101 atcgttgacc aggtttttgg tggtctcgtt accgtgacgc agtgcgaaac cgaggccaat
     8161 tgcccacacc aggatcccga tgtagttacc tttcagcagc gcgtcgatgg ggttggaaac
     8221 catgctcatt accagcccgc gcatcacttc tacaatgcct gacggtggcg aaatatcacc
     8281 cgcgctactg gataagtgca gggtagaagg gaaggcgaag ctgaaaacga ctgcggccag
     8341 agcagcagag aaggtaccca gcagatagag gaacaaaata gggcggatat tggttttctg
     8401 cccgtgctgg tggttagcaa tagatgccat caccagcatc aacaccagga tgggggcaac
     8461 ggctttcagt gcgccgacga acaaagtacc taacagacca acagcttccg ccgcgggttt
     8521 tgagatccat gccagaagaa tccccagaac aaggccgacc aggatttgtt ttaccaggct
     8581 gccatgagcc agacgccgga atagccccgg tgaacgttgc gtagtcattt ttcgatcctt
     8641 tcattgtgtt gtcgcacatc cctggtgcgt tctgtcgacg catctttcag gatgtaacaa
     8701 agtgtttgca aggtcgagta taaggaaagt tacagcttcg ggaagcgaaa aatgctggat
     8761 tttaaagaga ggtcatattt tgcaacttaa ttgttacatt tgtttgacat aaacagaggg
     8821 aaggactggc tactaaggct gattagataa catatgggca aaactatttg gaatatgaga
     8881 acggttgcgc tcctttaatg tgctatgcca tataaagtca ctggcgcagg gagcgcgcag
     8941 ggggcagcca atcgccgccg ccccctgcac ccccaggctc tggcgaacaa aatcgccgct
     9001 gcgcgattcc ctcagcttat cccttgcggc taccgggtcg ggcgcaaggg aacgtccctg
     9061 taagatgcgc cctcgccgcg catccatgcg cgtcgccccg gcctccaggg aacgcttcgg
     9121 cgattttgac gccaccaaca cccgtgcggt ctttttgtaa agagctacaa gccaattaca
     9181 taattaatag ctcacctttg tctggaatga gttatctcta ggaatggctt aatatacagc
     9241 gaatcattct ccccgctgct tatcatgccg ataattcacc ctggcgttga taataaacgt
     9301 catcaccaga atgccaaaca ccacgcccag cgagacggcg attggaatat ggtagaagtc
     9361 gacaatcagc atcttgatac cgataaacac caggatcacc gccaggccat atttgagcat
     9421 cgagaaacgc tctgctacgc ccgccagcag gaaatacatc gcacgcaggc cgaggatcgc
     9481 aaacaggttt gaggtcagca caatgaacgg gtcagtggtc acggcgaaga tagccggaat
     9541 gctatccacg gcgaaaatca cgtcgctcaa ttccaccaga atcagtacca gcatcagcgg
     9601 tgtggcgtac agcaatccat tcttacgcac aaagaaatgc tcgttgtcga tggtgtcggt
     9661 catgcgcaaa tgaccgcgta gccagcgcac cagcggcttg tcgccaatgc ctgattcatc
     9721 ttcatgggcg agcgccatct tcacgccggt aaacagcagg aaggcaccga agatatacag
     9781 gatccagtcg aactgcgaaa ttagccagct gccagtgaag atcatgatgg tacgcagaac
     9841 aatcgccccg agcacgccat acaccagcac gcggcgttgt aatgccgccg gaacagagaa
     9901 atagctgaac aacatcagcc agacaaagac gttatcgacc gccagcgatt tctcaatcag
     9961 ataacccgtg agaaaggcca gtgcctgtgg atcggcaacg gcgcgacctt cggtttgcac
    10021 cagataccac cagaaagcgg cgttaaacag taacgacagc gtcacccaaa ccagcgacca
    10081 ggccgcagcc tgtttcatgg tcatggcatg tgccccacga cgcccctgca acaacaggtc
    10141 gatagccagc ataatggcga caacaacagc gaatccgccc catagcaacg gcgtgccgac
    10201 agtattcata gaagttcctt acacataaaa aaaacggcca acgtcggaag acgtcagccg
    10261 ctgcttttta tgcatagacc tcgccttccg gcaaggtctc acttacaaca aaaaaggaat
    10321 acgtctgcgt attcctttcg ggttgcccgg tgaccggatg tggtttttca cacatcgtaa
    10381 tgacgatcga ccggcaatga agttactccc ctttgcaggt aacaaagtat gacagaccat
    10441 tcagccagtc aatggtctgt tacatacctt ttacactgtt ttactttggc aattatagtt
    10501 ttacgctatc tgccggaaaa atcaccccag tctggcggcg gatctcagtc agcagtttgg
    10561 cggtgatgcg actgaccgcc agccctggat gatccaccag atgctcatcc accagggtag
    10621 caaacagctc tgcttcgtag agcatggtat taatatgctg cggctgggtg agatcctgca
    10681 tctggctgcc acgcggcacg aagcacactt tctggcattc agacagtttt tcaatcacca
    10741 gcgatcccgc ttcgccctga atctcgcttt ccaggacaga atcactgacc tttgagtgca
    10801 gcaacgtaac gctgaaatca ccgtaatcca tcaccaccac gccgtgggca tctacgccgc
    10861 ttgccaacaa actggcggtt gcctgcacgc ttttcggttc accgaataat gccaccgccg
    10921 acgccaggca gtaaaagccg atatccataa ttgaaccgtt agagaatgcc ggattaaagg
    10981 tgttgggatt ctcaccatcc aggtaacgtt gataccgcga ggaatactgg caatagttga
    11041 aaaagacttt acgcaatttg ccgactttcg gcagcgcctg gcgcagcaaa tgaaagttcg
    11101 gcaggctggc ggttttaaat gcctcaaaca gcaccacctg attttcccgc gcacaggcaa
    11161 tggcggcatc cacttccgcc agattcgacg ccagtggttt ctcgcaaatc acatgaattt
    11221 tatggctaag gaaaagttgt gtctgggaaa agtgcagggc attcgggctg gcaatataca
    11281 ccgcgtcaat ggcatcgctt tccgccatcg cttccagcga ggtaaacaga tgctcgacag
    11341 aaaaatcatt ggcgaagtgc tgggcctgtt caaggctgcg ggaatatacg gcggttaact
    11401 tgtatttacc gctctcatgg gcggcctcga cgaactggcg agtgatccag ttcgtaccaa
    11461 tcacagcgaa acgtatcata acgcgtggat actccataaa gggattctgt cgtcaatgta
    11521 gcacgccttt ttcatcttcg acgtgcgcag atacgcatta ttcttttgcc ggacagccat
    11581 gctcactaaa cggcccaatc atgaccgatg gcccgagata tttcggctgg ctatggagga
    11641 ttttggcgtc aatcacggcg ctgacacgag cgagtttctc tcgcagttga gtataaaaac
    11701 cgtgcttcat attgaggtca ggcgcgttaa aaccgatagc gtcgatgctg tactgttttg
    11761 ccagccagat ggcgcgttgg ttatgaaatt cttgcgagat aatggtgata tggttttcac
    11821 caaaaacctt tttggcacgc actaccgaat ccagcgttga gaatccggca tagtcgcaga
    11881 agatcacttt cgccggtacg cctttagcga tcaaagcctt ctgcatacct gatgcttcat
    11941 cgtaattttt acgtccgtta tcgccactta ccagtagcca tttcactttt ccggcgtggt
    12001 acaacgctgc tgcggtatca atacgtcggg taaagtagcg attacccggc ctggccccca
    12061 gcaataagcc cacgttacgc gccggaactg cgttgacatc gctccaggtc agctgcttgc
    12121 tggcgttgac cattactcga tcggcgataa aaatggtcgc tcctgccaca agtaacagta
    12181 agcaggcaat ctttagagtc cggcgagaaa aacaaatacg gagaagaagg cggggctgca
    12241 tggtcgttat ccttaacgaa aggggggagg tcgattttag gcgctaaaga aagaaaagat
    12301 aacgcctgac atcattttca gattttgtcc tgcccaagcg acaactgcgt tagccacagc
    12361 cgggtgtcga actccagctg gtggtactgc ggctccatat ggcagcacaa ctggtaaaac
    12421 gctttgttat gctctttctc cttcaggtgc gccagttcgt gcaccacgat catacgtaaa
    12481 aacggttccg gcgcgttgcg aaacacagta gcgacgcgga tctctgcttt tgctttcagc
    12541 ttgccgccct gcacacgaga aacagcggta tgtaacccga gcgcgttttt tagcacatgg
    12601 atcttattgt catacatcac tttattgatc ggcggggcat tgcgcagaaa ctgatttttt
    12661 agatcctgag tatattgcca gagggcttta tcggtggcgt agtcgtgtgt tcccggatag
    12721 cgttttgcca gcacatcgcc cagacgctgt tcgttaatca gcgttcgcac ctgggaaagt
    12781 agctgctcgg gataaccctg aagataagta agattgctca taaccgccca cgaaaaagag
    12841 aaaaagggta tactcacgca cccttttcag gggcaacgtc gaaattttat cattcaggag
    12901 ggccgatgag ccacttagac aacggtttcc gttcactgac actacaacgt tttccggcga
    12961 cggatgacgt taacccgcta cagtcgtggg aagcggcgga tgaatatttg ctgcaacagt
    13021 tggacgacac agaaatccgc ggcccggtgt tgatcctgaa tgatgccttt ggtgcgttaa
    13081 gctgcgcgct ggcggaacat aagccgtaca gcattggcga ctcatacatc agtgaactgg
    13141 cgacgcgcga gaatttacgc ctcaacggga ttgatgagtc gagcgtgaag tttctcgaca
    13201 gcaccgccga ctacccgcaa cagccgggtg tggtgctgat caaagtgccg aaaacactgg
    13261 cattgctgga acaacaactg cgtgcgctgc gcaaagtggt cacgtcggat acacgtatta
    13321 ttgccggtgc caaagcccgt gacattcaca cttccacgct ggaactgttc gaaaaagtgc
    13381 tcggcccgac caccaccacg ctggcatgga aaaaagcgcg cctgattaat tgcacgttca
    13441 atgaaccgcc gctggccgat gcgccgcaga ccgttagctg gaagctggaa ggtactgact
    13501 ggactatcca taaccatgcg aatgtcttct cccgcaccgg gcttgatatc ggcgcgcgct
    13561 tctttatgca acatctgcca gagaatctcg aaggggagat tgtcgatctc ggttgcggta
    13621 atggcgttat tggtctgacg ctgcttgata aaaatccgca ggcgaaagtg gtgtttgtcg
    13681 atgaatcgcc gatggcggtt gcttccagcc gtttgaacgt tgaaaccaac atgccagagg
    13741 cgttggatcg cagcgagttt atgatcaaca acgcgctctc cggcgtggag cctttccgct
    13801 ttaatgctgt gctctgcaac ccgccgtttc accaacaaca tgcgctgacc gataacgtcg
    13861 cctgggagat gttccaccat gcccgccgct gcctgaaaat caacggcgag ctgtatattg
    13921 ttgccaaccg tcacctggat tacttccata aactgaagaa gattttcggc aactgcacca
    13981 ccatcgccac gaataataaa tttgtggtgc tgaaagcagt gaagctgggg cgtcgtcggt
    14041 aagattggga gttacctgcc ggatgcggcg ttaacgcctt atccggccta cgttcagcac
    14101 gttactccca ggccggataa gacgcggcaa gcgtcgcatc cggcaatgtc tgcccagttc
    14161 tggaatcgcg cttccataaa acaaaaaatc cctctcacca tcctctacat tctcttgttt
    14221 agcgtttttc tacgtttatt cttccgtcac acagataaat tcatacgttg ccaatctgtc
    14281 aacggatgtt atcatattgc caatttgtca acggacgtga tgaagaatgc acctgataac
    14341 tcaaaaagca ttgaaagatg ctgcggaaaa atacccgcaa cataaaacgg agttggtggc
    14401 tctggggaac atgattgcta agggatattt caaaaaacct gagtcattaa aagcagtatt
    14461 cccatctctg gataacttca aatatctgga taagcattat gttttcaatg ttgggggcaa
    14521 tgaattacgt gttgtagcaa tggtcttttt tgaatcgcaa aagtgctaca tacgtgaagt
    14581 tatgacgcat aaagaatacg atttctttac cgctgttcat cgtactaagg ggaaaaaatg
    14641 attgcgattg ccgacatctt gcaagcagga gaaaagctaa ctgcggtggt accttttctg
    14701 gcgggtattc agaacgagga acaatacacc caggcgctgg aactggtaga tcatctgctg
    14761 ctcaacgatc ctgaaaaccc cttgctggat ctggtgtgtg ccaaaataac cgcgtgggaa
    14821 gaatcagcgc ccgaatttgc ggaatttaat gccatggctc aagccatgcc tggcggtata
    14881 gccgtgattc gtacccttat ggatcaatat ggtttaaccc ttgccgatct gccggaaatt
    14941 ggcagtaaat ctatggtgtc acgcgttttg agcgggaaga ggaaattaac gctggaacac
    15001 gctaaaaaat tggcaacgcg attcggcatt tctcccgcct tgtttattga ttaacgcgac
    15061 gtgccggatg cgttgcatcc ggcgcttcaa ataacgattt aaatctccag cgccagccgt
    15121 gttccctggg caattgcccg tcgtgcgttc agctccatag ccacatcgca gccgccaatt
    15181 aaatgcacag ttttcccgct atcaatcagc ggttgcgcca gcgcgcggtt tggctcttgc
    15241 cctgcgcaga tcaccacatt gtccacagct aatacctgcg tttcgccgtt gatcaccaca
    15301 tgcagcccgt catcgtcaat cttctgataa cttacgcctg ggatcatttt cacaccccgc
    15361 gagagcaggg tggtgcgatg gatccagccg gtggttttgc ctaacccctg tcccggtttg
    15421 ctggctttgc gctggagcat cacaatttgc cgtgggctac gcgggatctg cattccctgc
    15481 gggcttaagc caccagcctg ttgtaggctg ctgtcgatcc cccactcatt acagaacctg
    15541 gcgatattct ggctggtgga ttcgcccggc tgacttaaat acatcgccgt atcaaaacca
    15601 atcccgccac aaccgatgat ggcaaccttg ttgcctaccg gcgctttgtc gcgcagtaca
    15661 tcgagataac tcaatacctt cggatgatcg atcccgtcga taggcggaac gcgcggcacg
    15721 atcccactgg cgaggatcgt ttcatcgaac gcctgtaact gatccgccgt cacggtgtga
    15781 ttgagtttca gcgtcacgcc cgtcacttcg atcatccggt ggtaatagcg cagcgtttcg
    15841 taaaactcct ctttgccggg gatctgtttg gcgatattaa actgcccgcc gctctcgctg
    15901 tgggcatcaa acaatgttac ctgatgccca cgcgccgccg cgttaatggc aaacgccagc
    15961 ccagcaggtc ccgcaccgac caccgccaga ttttttttct gcacggcagg aaggattggc
    16021 attttggttt cgtggcaggc gcgaggattc accaggcacg aggtgacttt gccaacgaag
    16081 atctgatcga ggcaggcctg attgcagcca atgcaggtgt tgatctcatc ggctcgcccc
    16141 gattgcgctt ttgacagcag ttccgcatca gcaagaaacg gtcgtgccat cgataccata
    16201 tcggcatcgc cgcgcgagag aatatcgtcg gcaacctgcg gatcgttaat ccggttggtg
    16261 gttaccagcg gcagcgagac gtggcctttc agtttgcgcg tgacccagct aaatgcgccg
    16321 cgcggcacgg gcgtggcaat ggtcggaata cgtgcttcat gccagccaat gccggtgttg
    16381 ataatggtcg cgcccgccgc ttcaatggcc tgcgccagct ctaccgtttc ggcaaaagtc
    16441 ccgccgtctt cgaccaggtc gagcatcgac agtcggtaga taataatgaa gtcgttgccg
    16501 acgcgttcgc gcaccgcacg cactacttct acggcaaatc gcatccggtt gcggtaatcg
    16561 ccgccccact ggtcactacg ctgattggtg tgcagcgtca gaaattcgtt gatcaaatac
    16621 ccttcggacc ccattacctc tacaccgtcg tatcctgcct cccgtgccag ttgcgcgcag
    16681 tgggcgaaat cgtcgatcag ttgcaggatc tcttcatggg tgagttcgtg aggaacgaaa
    16741 cggttgatgg gggcctgcaa tgcggaggga gcgaccagat gcggttggta gctgtagcgc
    16801 ccggtatgca aaatttgcag ggctattttg ccgccttcct gatgtaccgc ttcggtaatg
    16861 gtgcgatggt gtgggatctg gctggcgtcg ttgagcattg cgccaccttc catgccaacg
    16921 cctgttaaat ctggtgcaat gccgccgctg acaatcagcg ccacgccgtg acgggcgcgt
    16981 tcggcataaa acgctgccag ccgctcggca ccgtccgggt attcctccag cccggtgtgc
    17041 attgagccca tcaacacgcg gttttttaac gtggtaaaac ctaaatccag cggggcgaac
    17101 agcgacgggt agctcataaa attgtccagt atgtaaaata attgttatgt ggtcggatga
    17161 gttctaattt agccgtcgcc ggagaaaagg gaaaagggga gtgcggggtt tgtgatggga
    17221 ttcaaaaaga atacgcccgg tggtaacgcc ggatgcgacg cttgccgtgt tttatccggc
    17281 ctgggggatt gcacatgtag ggcggataag gcgttcacgc cgcatccgcc agtggcgcgg
    17341 tgcagatgcc ggatgcgacg cttgtcgcgt cttatccgac ctacgggaac gcacatgtag
    17401 ggcggataag gcgttcacgc cgcatccgcc agtggcgcgg tgcagttgcc ggatgcaacg
    17461 cttgccgcgt tttatccggc caggaggatt gcacatgtag ggcggataag gcgtttacgc
    17521 cgcatccgcc agcagttgca aattattgct tacggaaaaa atcgttatac aacatataca
    17581 aatgcgcggc actccaggag aaatttggtg cgccttgctg cgcgccagtc agcgggttgt
    17641 aattctcctg aattgggcca tcggcggtta atcctttggc gtgccggaag aacgtatccg
    17701 ccagcttcag ggcatcatca cgataaccgt aacgctccat ccctttcaga gcaaaccaga
    17761 actgatccac ccatacgcgc ccgcgccagt agatatcagc gccaaaagcc ggattggtta
    17821 acgccgccgt tcccagcggg acaaaggtgt tgaactcttt aggatcgagc atcaccttca
    17881 ccaccgcgtc ggcattggcc tgcgttgccg caccgttaaa cagcggcgac cagccttccg
    17941 gccctttacc gcgctcaaca atcggtttgc ccgcgcagcc gttcgccagt ggtttatctt
    18001 caatacgtac gtcatagtag aactgggtag tcgggtcgaa catacaggtg ttgatgtagt
    18061 ccgcgagctg ctgtgccaac tggcgatagc gtttggcctc ttctggctta ccgaggatgg
    18121 ttgccatctc cgccagataa tggttatcgc tgtacatata gctggcctga tccaccgact
    18181 cctgcaatag cgagtagccc agcaacgttc cgtcctgact gcggttttcg gcgaatttca
    18241 ccgtccagtc gctacgtttg ccaccattag cgacatattt atccaactgt tctttgtcga
    18301 taaaaccaaa gacggcggca tcatcacgcc ccgattccca cgacgcagcg acctgtgccg
    18361 gaatttccag actgtcgtac tggcctttct ccaccacgcg ggcgtagttg ttcagcccag
    18421 actgcgtctc ttctttgttg cctttcttca ccgcaaacag catctcgccg ctctcagtgt
    18481 tgtgggcttt gtcgcgggtc gcgccatatt ccggcacgcc gttgccgttg tgatcgcggt
    18541 tacgtaacca ccagtcgtga taggcgacca gtttcgggta catctctgcc aaccaggctt
    18601 tatcctgggt gacgttgtac acttctatca ccgaccaggc ggcaaggctg ggtttggtat
    18661 tacgttcgtt ccagttgccg ccatcgccac cacgctcggg gctaaggttc cacgctatca
    18721 ggtcggggac aaagcccaca tcctgcggac gcacgcggtc gccaggctgg atctgccagg
    18781 agaagaccgc gcggatattc tctttggcga tgtccggatt gaaatgcgcc atcgcaaacg
    18841 cctgcttcta ggtatcccac ggccaggtct gattgccgga gaaccagcgc ccggtcaccg
    18901 acggtgtaac ggtgttaaat ttcaccgcac cgcccggtga gcgccagtta ccgttgagcg
    18961 tttcgatcgc tttcaccgcg acgcgcgtct gttccggcgt cgcatccgga ttggttaacc
    19021 ctttcttcag gtattcttcc cagcgttgct gcgaggcggt gagataaaac gccggacgcg
    19081 ccagaatatc gcggatctgc atttgctctt tgctaacttc ctgagcggtc agcagatggg
    19141 aataggtggt gtagagcgtg gtcgaaccgt tgatatgcgc cttactggta aagcgattac
    19201 cgttgatttc agtctgcacc ggcagggatt tatgcacctg atattccgat tcgccggagg
    19261 tcagcagatc ccaggtggcg cgcactttgc caaaggtgac tttcaggcca tcacgggtgg
    19321 cgctgatttt gcgctgatag tcagggtatt cgccagcaat ggttttatcg gaaagcggtt
    19381 tcccttcttt cgcttccagt ttttccagca gttcgccgtc ccacaccaga tccagcggtt
    19441 tattgctggt gattttggtt tccagtagtg acgtgcgcgg cgtggcgaag cgcagagtca
    19501 tttcgacttg cacatctttt gctgtcagtt tttgcaccag cgcaccggga atactgtatg
    19561 cctccagcgt aaagtcgact ttcttgccgt cctgccagac ggtcaggcgg tcgaaattgc
    19621 tggccataaa gttgatgtac tcttccgtca gcagcgcaac gcccggaaag ccgcccatgg
    19681 tgttagggcc gtctggcaac agatgaccat gccaggctcc gagatcgaaa aacggattaa
    19741 aacgctggtg atcgtcgtaa tcgtaatcct tcatgtactg cggcgcgcca gtacggttaa
    19801 tcacgttttt ataattgtcg gcgttagcgg catgggcgga aaaacttatc agcagagcgc
    19861 aggttactgg cgttaaaata gtttttattt tcatgaaatt acagtcctta ttaccagtag
    19921 aaatattgca tcaggaagat ttggtcgctg ggatcgtgag aggcattttt catgtagaag
    19981 cggctatcaa aagccgtggc gaaacgtccg ccgccgtatt cgtaccagat accaaactgc
    20041 atgaaagagg tggtttcatc gtcagcgtgg tcagggcgat gtttggcgta ggtgtaagcg
    20101 gtggagagat aaacgccctt taacgcttca tacatggcgc tcaccatcat cccactttca
    20161 cttcccggta cgtccagccc gtcggcgcgt ccggtatggt gccaggcgcg agcggcaaag
    20221 ttaggagtta gcaggcccgt gagatagagc tgtcctgtgc cgtcggtgat ttccagaccg
    20281 ttcatccagg tgatgccttc ggcaaggtca tactggatgt aaccgttcat cattgccgga
    20341 taggtgtatt tgtcgtcata gcgatcgtat ttgccaaaat gtagccaggc tttgctttca
    20401 tcatgatgtc cggcaggagt cgcggtgacg ctataacgaa agttgccgct taagttctgt
    20461 accttcagcg cagtcataat gtcgcgggta ttgggaatga cgtagcccag atcgggggtg
    20521 aaatcgcccc accattgcag gtcatccagc gaggaatcct gacgcagact gagcatgatt
    20581 tcggtgccat cattggtacg ataaccgcca tagaaacggt taattccgcc ctcgaaacca
    20641 ccccagctat ggtctgg
//
LOCUS       DADLFA010000061        20603 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000061 DADLFA010000000
VERSION     DADLFA010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20603
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.061"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(56..481)
                     /gene="nudI"
                     /locus_tag="JQH65_001880"
     CDS             complement(56..481)
                     /gene="nudI"
                     /locus_tag="JQH65_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HAY6139312.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEYGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYTWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            760..1302
                     /locus_tag="JQH65_001881"
     CDS             760..1302
                     /locus_tag="JQH65_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6139313.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            1402..2604
                     /locus_tag="JQH65_001882"
                     /pseudo
     CDS             1402..2604
                     /locus_tag="JQH65_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
     gene            2824..3606
                     /locus_tag="JQH65_001883"
     CDS             2824..3606
                     /locus_tag="JQH65_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAY6139314.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLSWQPAAVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYPDVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKSIACARDISRLLGWKSPFDLQAS"
     gene            3621..4826
                     /gene="rhmD"
                     /locus_tag="JQH65_001884"
     CDS             3621..4826
                     /gene="rhmD"
                     /locus_tag="JQH65_001884"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HAY6139315.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLVVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            4883..6172
                     /locus_tag="JQH65_001885"
     CDS             4883..6172
                     /locus_tag="JQH65_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY6139316.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVMAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            6190..6993
                     /gene="yfaU"
                     /locus_tag="JQH65_001886"
     CDS             6190..6993
                     /gene="yfaU"
                     /locus_tag="JQH65_001886"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HAY6139317.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTSYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVSSATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(7034..7936)
                     /locus_tag="JQH65_001887"
     CDS             complement(7034..7936)
                     /locus_tag="JQH65_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HAY6139318.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(8129..9319)
                     /gene="glpC"
                     /locus_tag="JQH65_001888"
     CDS             complement(8129..9319)
                     /gene="glpC"
                     /locus_tag="JQH65_001888"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HAY6139319.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(9316..10575)
                     /gene="glpB"
                     /locus_tag="JQH65_001889"
     CDS             complement(9316..10575)
                     /gene="glpB"
                     /locus_tag="JQH65_001889"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HAY6139320.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLH
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLLCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPHFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(10565..12193)
                     /gene="glpA"
                     /locus_tag="JQH65_001890"
     CDS             complement(10565..12193)
                     /gene="glpA"
                     /locus_tag="JQH65_001890"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HAY6139321.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            12466..13824
                     /gene="glpT"
                     /locus_tag="JQH65_001891"
     CDS             12466..13824
                     /gene="glpT"
                     /locus_tag="JQH65_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HAY6139322.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            13829..14905
                     /gene="glpQ"
                     /locus_tag="JQH65_001892"
     CDS             13829..14905
                     /gene="glpQ"
                     /locus_tag="JQH65_001892"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAY6139323.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(14947..15189)
                     /locus_tag="JQH65_001893"
                     /pseudo
     CDS             complement(14947..15189)
                     /locus_tag="JQH65_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            15368..16018
                     /gene="inaA"
                     /locus_tag="JQH65_001894"
                     /pseudo
     CDS             15368..16018
                     /gene="inaA"
                     /locus_tag="JQH65_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
     gene            complement(16072..16326)
                     /gene="yfaE"
                     /locus_tag="JQH65_001895"
     CDS             complement(16072..16326)
                     /gene="yfaE"
                     /locus_tag="JQH65_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HAY6139324.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(16326..17456)
                     /locus_tag="JQH65_001896"
     CDS             complement(16326..17456)
                     /locus_tag="JQH65_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase 1 subunit
                     beta"
                     /protein_id="HAY6139325.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDSEVDTDDLSNFQL"
     gene            complement(17603..19888)
                     /gene="nrdA"
                     /locus_tag="JQH65_001897"
     CDS             complement(17603..19888)
                     /gene="nrdA"
                     /locus_tag="JQH65_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HAY6139326.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINNLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGECP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
BASE COUNT         4846 a         4992 c         5525 g         5240 t
ORIGIN      
        1 aattcgttaa ccactaaatt ttcaatctga cagccagtga tggctgtcat cattgttaaa
       61 gaagaccttt caaacgtaac gtttttcggg tggcaacatt caaatcataa tgcaccagat
      121 cttcaggttt tacccacgtg tagtcctgaa actcttcgtt tattttcact tctcggttgg
      181 cagaaacgca gtcaaaaatc aggtaaatca tataaatctc ttccttgcga ccatctgcat
      241 acgtcttggt gcgaatatca tcgctgaagg tccacggcgt gatttctgtc aaaagcagct
      301 gttctcccag ttcttcgcga atttcgcggc gtagtgcctc ttcaattcgt tcgccatact
      361 ccacgccgcc acccgaaagc gcccattgac cggggaaaac gccgcgatcg tcggccattt
      421 tgcacagcag ataagcacca tcattttgaa tcaaagggca tacaatagtc cgttgtcgca
      481 caggggtatt ccttataaat aaaaagaaac catgagaata cggtaactca gaaatatgtg
      541 aaagtgacag taatctcttt tgcgatgata actgcactta ttattatttt tgtgtgtatc
      601 ggtaaagtgt gtttattttt tcggcgttta attatttaag aagtatttag ttacaataag
      661 tgactagatc ccttttttcg ctcattgaat cctctgtgcc aggcagtaca atcccgcagc
      721 tttgggaatt ttgattaatt gcttgtggga aaggggataa tgaaaaaaat tgcgctagca
      781 ggtctggccg gaatgctgtt ggtttctgca tcggtcaatg caatgagcat cagcggtcag
      841 gcgggtaaag aatacaccaa tattggtgtc ggttttggga ctgaatcgac gggcctggcg
      901 ttaagcggta actggacaca taacgacgac gacggtgacg tcgctggcgt ggggctgggg
      961 ttgaatctgc ctctagggcc gttaatggcg accgttggcg gaaaaggcgt ttacaccaac
     1021 ccgaattacg gcgatgaagg ttatgcagca gcggtaggag gtggtttgca gtggaaaatt
     1081 ggcaacagct tccgtttgtt tggcgagtat tactactctc cggattcact ctccagcggt
     1141 attcaaagtt atgaggaagc gaatgctggc gcgcgttaca ccattatgcg tccagtcagt
     1201 attgaggcgg gttatcgcta cctgaatttg tcgggtaaag atggtaaccg cgacaacgcc
     1261 gtggctgacg gcccgtatgt tggggttaac gccagtttct gatcccttcc ggcgcggcat
     1321 tctgtcgcgc cattcagttt cttactacgc cttatctccc tttgcgttat agtgattcga
     1381 taacaaaagc aggagataac catgttaaaa gtggaaatgt tatccaccgg ggatgaagtg
     1441 ttacacgggc aaatcgttga cactaacgct gcctggctgg ccgatttttt ctttcatcag
     1501 gggctgccat tatctcgcct caatacggtg ggggataacc ttgatgactt agtcaccatt
     1561 cttcgcgaac gtagtcagca cgccgatgtg ctgatcgtta acggcgggct gggaccgacc
     1621 agcgatgatt taagcgcact cgccgctgcg acagcaaaag gtgaaggcct ggtgctgcat
     1681 gaagcctggc tcaaagagat ggaacgctat ttccacgaac gtggacgagt aatggcaccg
     1741 agcaaccgta aacaagcgga gctgcctgcc agtgctgaat ttatcaataa cccggtaggc
     1801 accgcctgtg gttttgccgt gcagcttaat cgttgcctga tgttctttac tcccggcgta
     1861 ccgtcagaat ttaaggtgat ggtcgagcac gaaatcctgc cgcgcctgcg cgagcgtttt
     1921 tctttaccac agccgccggt ttgtctgcgt ttaactacct ttggtcgttc ggaaagcgat
     1981 ctggcacaaa gcctggacac tctacaactg ccgccgggcg taacaatggg ctatcgctct
     2041 tcaatgccga ttatcgaact gaaactcacc ggaccggcaa gcgagcaaca ggcgatggaa
     2101 aaactgtggc tggacgttaa acgtgttgcc ggacagagcg tgattttcga aggcaccgaa
     2161 ggactgcccg tgcagatcag tcgcgaattg caaaaccgcc agttcagcct gacgttgagc
     2221 gagtaattca ccggtggttt attggctttg caactttctc gcgcaggtgc tccattgctg
     2281 gcgtgtgaag tggttccttc acaggaagaa aacctggcgc aaactgcgca ctggattaca
     2341 gaacggcggg ccaaccattt tgccgggctg gcactggctg tttcgggttt cgagaacgaa
     2401 catctcaact ttgcgctagc cacaccagac ggcactttcg ctctgcgtgt gcgtttcagc
     2461 actacgcgct acagcctggc tatccgtcag gaagtgtgcg caatgatggc actgaatatg
     2521 ctgcgccgtt ggttaaacgg ccaggatatc gccagtgagc atggctggat tgaggttatt
     2581 gagtccatga ccttatctgt ctgattccgc cactctgtgc cggtttactg caccggcaca
     2641 ttccccctcc ggcaaaactt gtccaacctg ttgcactcaa taagaaaaaa tccgtgcact
     2701 gattcacaaa tttgtgcgta atttctccag tccgctggac gaggagcggg atttgatgcg
     2761 cttcacaatt ttactccccc tgagcaacga gactgattcc agtatctaag actggagtcc
     2821 agtatgttgg aatcaagcaa agttcccgcc ctgacacggg ctattgatat tctcaatttg
     2881 attgcccgca ttggcccctg tagtgctgcg acgatcattg acacattggg aatccctaaa
     2941 agcacggcct atttgctgct taatgaactc aggcgtcagc gttttcttag ccttgatcac
     3001 caggaaaatt tttgtttgtg gaccaggctg gtggagcttt ctggtcatgc gttaagcaag
     3061 atggatctcc gggaactggc tcgaccgcgt ttgacgcaac tgatggatac caccgggctg
     3121 ttatgtcatc tggggattat tgataacggc agcgcttatt acattttaaa agtggagtca
     3181 tcagccacta tcagcgtgcg ttcccatgaa ggaaaaagcc tttcgcttta tcgctccgga
     3241 ataggtaaat gcctgctttc ctggcaacct gcagcggtac agcaaagtat tatcgaaggg
     3301 ctggtctggg agcaggcgac acccacaacg attactcatc cacagcaact tcacgaagaa
     3361 ctggcgcgta ttcgccgtca gggctggagc tatgacaacg gcgaagatta ccccgatgtc
     3421 cgttgtgtcg ctgcgccggt gtttaacgcc aataacgagc ttaccgctgc gatctcggtg
     3481 gtcggtaccc gcttacaaat caatgaagaa taccgtgatt atctggcggg caagtccatt
     3541 gcctgcgcca gggatatttc acgtctgttg ggatggaaaa gtccctttga cttacaagcc
     3601 tcataacaat ggagaacatc atgaccctac caaaaattaa acaggttcgc gcctggttta
     3661 ctggcggtgc gacagcagaa aaaggcgctg gcggcggtga ttatcacgac cagggggcga
     3721 atcactggat agacgatcat attgccaccc cgatgagtaa ataccgcgat tacgagcagt
     3781 cacgccagtc atttggcatt aacgttcttg gcacgttggt tgttgaagtc gaagcagaaa
     3841 acggccagac cggattcgct gtttcgacag ccggtgaaat gggctgtttt atcgtcgaaa
     3901 aacaccttaa ccgtttcatt gagggtaaat gtgtcagtga tatcaaactg atccacgatc
     3961 aaatgctcaa tgccaccctg tattactccg gctctggtgg cttggtgatg aatacgattt
     4021 cctgtgtcga tctggctctg tgggatctgt tcggcaaagt ggtcgggctt ccggtttata
     4081 aacttttagg cggcgctgtt cgtgatgaga ttcagttcta cgccacaggt gcgcgtccgg
     4141 atctggcaaa agagatgggc tttatcggtg gcaaaatgcc gacgcactgg gggccacatg
     4201 atggcgatgc ggggatccgc aaagatgccg ctatggtcgc ggatatgcgt gaaaaatgcg
     4261 gtgaagattt ctggttaatg ctcgactgct ggatgagtca ggacgtgaac tatgcgacca
     4321 aactagccca cgcttgcgcg ccctataacc tgaaatggat cgaagagtgc ctgccgccac
     4381 agcagtatga aggttatcgc gaactgaaac gcaacgcgcc agtcggaatg atggtcacca
     4441 gcggtgagca ccacggcaca ctgcaatctt ttcgtacgct ttcagaaacc ggtatcgaca
     4501 ttatgcagcc ggatgttggc tggtgcggtg gtttaaccac gctggtggaa attgccgcaa
     4561 tcgccaaatc cagggggcaa ctggtggtgc cgcacggttc gtctgtttac tctcaccatg
     4621 cggtgatcac cttcaccaat acgccattca gcgaattcct gatgaccagc ccggattgtt
     4681 caacgatgcg tccgcagttt gacccgattc tgctcaatga gccggttccg gtgaatggtc
     4741 gtattcataa atcagtgctt gataaacccg gttttggcgt cgaactcaat cgtgactgca
     4801 atttgaaacg cccctacagc cactaatcac tccgggcgtt gctgtgacaa cggtaacgcc
     4861 cacatattct aaggagcttt ttatgagcac cgctttgctt gacgccgtgg tgaagaaaaa
     4921 ccgtgtgcgt ttaattccgt ttatgttggc gctgtatgtg ctggcgtttc tcgaccgttc
     4981 gaatatcggt tttgccaaac agacctacca gattgatacc gggttgagta atgaagctta
     5041 tgcgctggga gcaggcattt tctttgtggt atatgcgttt ctgggggttc cggcgaatct
     5101 tttgatgcgc aaactggggg ccagaacctg gattggtacg acaacactgc tgtggggatt
     5161 tctttcggca gccatggcat gggccgatac tgaagcgaaa tttctgatag ttcgcactct
     5221 gcttggtgct gcggaggccg gatttttccc tggtatgatt tatctcacct cgcaatggtt
     5281 tccgcagcgt aatcgcgcca gcattatggg gctgttctat atgggcgcac cgctggcgtt
     5341 aacactggga tcaccgcttt ccggcgcgct gttggagatg catggattta tggggcatcc
     5401 cggctggttc tggatgtttg tgattgaagg attgttggca gtcggtgctg gggtattcac
     5461 attcttttgg cttgatgaca caccggagca ggcacgtttt ctgagtaaac aagaaaaaac
     5521 gttgcttatc aatcaactgg caagtgaaga acaacagaaa gtgacttctc ggctgagcga
     5581 tgcgctgcgt aatggccgag tctggcaact agcgattatc tacctgacca ttcaggtggc
     5641 ggtttacgga ttaattttct tcctgccgac ccaggttgcg gcattgctgg gaacaaaagt
     5701 gggctttaca gcgtcggtgg tcaccgccat tccgtgggtt gcggccttgt ttgggacctg
     5761 gcttattccg cgctattccg ataaaaccgg cgaacggcgt aatgtcgcag cgctgacatt
     5821 actggcggcg ggcattggta ttggtctgtc cgggctgctt tctccagtaa tggcgatcgt
     5881 agcgctgtgt gttgcagcta tcgggtttat tgccgtgcag ccggtgttct ggacgatgcc
     5941 gacacaactg ctttccggta cggcgctggc tgcggggatt ggttttgtaa acctgtttgg
     6001 tgcagtgggc gggtttattg ccccgatcct gcgcgtgaaa gcagaaacgt tatttgccag
     6061 cgatgcggca ggattactga cactggcagc ggtggcggtc atcggttcgc tgattatttt
     6121 cactctgcgt gtaaatcgta ctgttgcgca gaccgacgtg gcacatcatt aaataggtta
     6181 aggaacacga tgaacgcatt attaaccaat ccctttaaag aacgtttacg caagggcgaa
     6241 gtgcaaattg gtctgtggtt aagctcgact acttcttata tggcagaaat tgccgctact
     6301 tccggttatg actggttgct gattgacggg gagcacgcgc caaacaccat tcaggatctt
     6361 tatcaccagc tacaggcggt agcgccctat gccagccaac ccgtgatccg tccggtggaa
     6421 ggcagtaaat cgctgattaa acaagttctg gatattggcg cgcaaactct attgatcccg
     6481 atggtcgata ctgccgatca ggctcgtcag gtgtcgtctg ccacgcgcta tcctccctac
     6541 ggtgagcgtg gtgttggggc cagtgtggca cgggctgcgc gctggggacg cattgagaat
     6601 tacatggcgc aagtcaacga ttcgctttgt ctgttagtgc aggtggaaag taaaacggca
     6661 ctggataacc tggacgagat cctcgacgtc gaagggattg atggcgtgtt tattggacca
     6721 gcggatcttt ctgcgtcgtt gggctacccg gataacgccg ggcacccgga agtgcagcga
     6781 attattgaaa ccagcattcg gcggatccgt gctgcgggta aagcggctgg ttttctggct
     6841 gtggctcctg atatggcgca gcaatgcctg gcgtggggag cgaactttgt cgctgttggc
     6901 gttgacacga tgctctacag cgatgccctg gatcaacgac tggcgatgtt taaatcaggc
     6961 aaaaatgggc cacgcataaa aggtagttat tgatatcaaa ggcccatggg gatcggctgt
     7021 gggcctgtgt taattagtgg ttattcgctg ccagatcggc ttcgcttagc tgggtggccg
     7081 cgagcacctg gtcacgatca atgccctgtt ccagcatgcg caaagcaatt ttaatggctt
     7141 gttcatgcat accttcctgc cagccaattt gatgcccttc ctgccgtagc cgttcagcaa
     7201 tagtcattaa tctctccttt tgtagtggtg aacgttcggc aatctcctca ataaaacggg
     7261 tgaaacggga ggtatcgccg cattgcagca gataattaaa cagtgtttgt agctggctgt
     7321 cattagtgaa gcctctaacc aaaagcgtgg tgatcctgtc gaccatgccg attaaatcgc
     7381 ggtcgcgaat atgcttttga atcagttcca gcagagctat acgccgatgt tgcatgatct
     7441 catcgtcagg tacgatggtg atatccacca acggaaaagc ttcggtgtac aactgccggg
     7501 caagttgcgg atcgtcaaac tcatccagcc agttaagtga gtacgggtag ggcgatgttt
     7561 cgccatgata aaacagcaat ggcaccacca gcggaactct gtcatagcct ttatccaggt
     7621 gacgctgcat ggcggcagtg gcatagcgca ttagccgaaa agccatattc ttttctgcag
     7681 agctttgatg ttcaatcacg cagtagatat agccgtcacc gtcgctggtt tccacggacc
     7741 acaaaacatc cgagtagtaa gcgcgtaaac ttttttcaat aaaactagtg ggttccaggc
     7801 gtaaggtttg caggttgcaa agcttgcgca gtggttctgg taaatgtatt tcgagaaaat
     7861 cccgtgcggt ttcgggtgtg aacataaagg ttttaaatac cgcatcatgc ggggaggagg
     7921 ttgttgattc ggtcatggtt gtccgtcacc tgatatttat gatgacgcga cattacggtt
     7981 atcctaggcc tttgacatct gccagtttca gcctttgcga gacgttttca gaggaatttt
     8041 cactgcgttg caacgcatac attgggcacg gaatcgtgta cgcaaaaatg cccggtaacc
     8101 acatgcttac cgggcatcag aaaggagttt aagccagcgc ctgggccagt agcgtaatcg
     8161 gatgttcgca gcgaagactt gtggacatct caatctgcca tttacaggtt tcgcagtcgg
     8221 tgaccaccag atctgcgccg ctttcttcta tctggcggaa cagtggtgcg ccgatggctt
     8281 gtgaggtggg gtagttctct tttttgaaac cgtaagtacc cgcaataccg cagcactggg
     8341 aatccagcac cgttaactca agccccggga ttttacgcaa cagctccagg gtgtagagcg
     8401 tccagcccat tttttccata tggcacggag tgtgataaac cactttcagc ggcagcggtt
     8461 tcagcggtaa cgttttgcct tcgtccagct tgcgccacag ccagcgggtt gccagttcga
     8521 tatgatcgcg caagcctttg ttgtcgacat tcagcacttc cgggtattcg tcgcgcaggg
     8581 caaatgtaca ggttgaggag gtggcaatca ccggaatgcc ttttactccc acagcttcgc
     8641 ggatcgactc tacattcgta attgcctgtt tgcgtgcttt atcggtaaag ccgttggcga
     8701 ttagcggtac gccgcagcat ttttctttgc tgagcagttg tacaccggta cccattgcgt
     8761 tgagcacttt aattaaatct ttacctaact gcggatggtt gtagttaacg aagcagccgt
     8821 gaaagaaagc gacctggtct ttatattgtg cttgctgagc cgctatgctg cgataccagc
     8881 gacggaacgt gccgaaggag tatttcggta gcgtgcgacg atgatcgatt tttaacgccg
     8941 catcaagcag ctgccgcacc ggtttcagcg aggtagcggt gttgacgatt ggtgcgaacg
     9001 gcgtggaaac gctacccatc aggtcggtat gactcaacac aaaattacgc agcgacgggc
     9061 gcgtggtgtc atattttgcc cgcgcgcgct ggataatatc gccaatcttc acatcggacg
     9121 ggcaggcgac ttcacaacgt ttgcagttga tgcaatattt cagcgcctcg tcatacagtg
     9181 cgccatcttt caaacgcaga cgctcgccat ccggcccggc ttgttttggc cctggataac
     9241 cgggattcac ccggctcacc gggcaggcgg tggtgcagac ggtgcactta atgcagtttt
     9301 cgaagctggt gtcgttcatt gttggcctcc tgcgcgttgg gcaatctgtt gagcggcatg
     9361 taaagcactg acggcacaaa caccgccgcc gcatccctgg gcgatgggat caaatccgcc
     9421 cagcaccgaa ccgatggcaa acaggttctc aatggtttgc cctgcctgcg acgggcgtag
     9481 cgtctcatca gtggttacac cgaactgctg ccacggttgc ggcgcaaaaa aatcgccctt
     9541 ataccattca ccccgcgtgg cggtttgtag cacatcaagg ccgagaatcg gctcgcgaat
     9601 gccgttacgt tccgctacca gtccgccact aaagaaactg ccgctggcga gaaccgcgaa
     9661 atgtggacgt agcggaatat cggcgtgatt gcgggtccag atttcattca ctacgccatt
     9721 tttacaggtc acttttttca cttcatcgcc cggcatccac acgccaccct ggcgcacaaa
     9781 ctggcgctgt aactggtttt gcagacgaat gcccagcacg gaaggcggca gcgttggcaa
     9841 aagcatcagt gaacaaagta gtttttcatt caaccaacgc cacagtttgt catcggccag
     9901 gccgaagcag gcaggcatca ggatcatttc gcaggtattg gcgacaggaa taagcgcatc
     9961 aagtaacagc ggccagtttt cttcattatc aaggaaacgg gcgatattca cagcgcgaaa
    10021 ttcggtggcg ttattgcgca ggacatccag ttccggcagc tctatttctg cggtttcaac
    10081 cgaaagatcc agttcgtgca acgaagccgc cgcaagatgc gcctgaaaat ccatcaggcc
    10141 gcttatcccg accacacata ttttcttcgc gggcaacggc cagacgggga cttctggcga
    10201 acttagccag gtagagcgca gagtgccgag cggcgtaatc cgctggtgag ccagttctac
    10261 gctgccctgc aattgcgcac cgctttcagc gattaatgcc tgcgcctggc aagcgagatc
    10321 gagcacgcgt tgtggcccga gaagagagta aggatgtgca ggtgcctgct gacgcaaaga
    10381 ttccagtcca ctgtgaatgt ctgccacggg ttgaccatct ggcagatggc tcagcaaatc
    10441 cagcgaaccg gatgagaaat gcaacgcgct ttgaccacgc gtgacaatgg cacagcgcag
    10501 gccgtgtttt tgcagttgca ggccacagag taatccggcg aggccgccgc ccataatgac
    10561 agtatcaaag cgcatctttc tgctccttct ccagaccaca cagtccctga taaacccagc
    10621 gggtaaattc gctttcgcgc agtgcgtctc cccaggcgat gggttgcacg cctttccagc
    10681 gttcgttaag gaaggtggaa agttgctcga tagattgcgc ggacgtcgtg acgttaaacc
    10741 gttgcagcag tccggcagcg cggcaggcgc agagttcgcc ctggcaggtg cccatcccca
    10801 cacgggtacg acggcgtaaa tccagcaggc tattaacgtt taaattttct accgcgtact
    10861 gcacttcacc cgcagttacc gcttcgcact cacataccag gctacggtgc agacggcctt
    10921 cgctcagcca ggcaggcgtg cgatcgccat gacgataaac cgcagaaccg cgcagcgggg
    10981 caggcaggga gatgacttta cgcaaggtaa cttcagcggg ttcttgtgaa ccaggcagtg
    11041 ccagatcggc agtcgtacag gggcgcgtgt tgcccagttt gcggcatacc gcgtcggtag
    11101 cccattcagc catcagccga taggtcatca gtttgccacc ggtgatggtg ataaatccgt
    11161 ccagaccatc gcgttcagca tggtcgagca gcacgatgcc acggctgacg ttacgtccgc
    11221 tcggatcgtc atcgctggca accagcgggc gcacgccaga ataggcccgc aaaatgcgcg
    11281 ttttcgccat cacgggggcc agtttttccc cttcacgcag cagaatatca acctcttctg
    11341 ccgtcactcg attatcgtca atctcgttgt aatcaatacg taaagaggtg gtaccaatca
    11401 gcgaaatggt atcgccaggc accagaatat cggcgtcgga aggtttacgg cagcggttga
    11461 tcacatgctg gttaatgcgg tgatccatga tcagcagcga tcctttcgcc gggaacatgc
    11521 gaatgcgcag atcggcatat tcggcaatgt gttgccccca gatcccagcg gcattaacca
    11581 cgacaggtgc atgaagggcc tgagtttcgc cggtgagatg gttacgtaca cgaacaccgc
    11641 acaccgtcgc gccttcacga atcagccccg tgacttcatg agcggtaagg ataacggcac
    11701 cgtgttcttt ggcatccagc atgtttgctg cggtcagacg aaatggatca acggtgccat
    11761 ccggaacttt caccgcgcca atcagtgccg ggttaacggc aggttcgata atgcgcgctt
    11821 gctgcgggtc tatagcttct gcgctgatcc ctgcttcttc gcaggcgcga ataaaagtgg
    11881 cctggaatga gaggtcatct tccggcaggg tgataaacag gccgttggtt ggttcaacgc
    11941 agtgacgtgc aatgcgtttc aggatctggt tttcactaat gcattcgcgg gccgattccg
    12001 catcggttac cgcatagcgc gcaccgctgt gcagcaggcc gtggttacgc ccggtggcac
    12061 cggttgcgat gtcgtggcgc tcaaccaaaa tcacgcgcag cccgcgcagg gcacagtcgc
    12121 gggcaatccc ggctcccgtt gcgccgccgc caatgataat cacgtcactt gattgcgagt
    12181 cgcgagtttt cattgttatc cctctgaagt tcgtttttta ccatttagcc atagtaaaaa
    12241 catgaattgt ttgatttcgc gcatgttcgc tcataattcg aaagtgaaac gtgatttcat
    12301 gcgtcatttt gaacattttg taaatcttat ttaataatgt gtgcggcaat tcacatttaa
    12361 tttatgaatg ttttcttaac atcgcggcaa ctcaagaaac ggcaggttct ctcactgaat
    12421 caggctgtta atcataaata agaccacggg ccacggaggc tatcaatgtt gagtattttt
    12481 aaaccagcgc cacacaaagc gcgcttacct gctgcggaga tcgatccgac ttatcgtcga
    12541 ttgcgctggc aaattttctt ggggatattc tttggctatg cggcttacta tttggttcgt
    12601 aagaactttg cgcttgctat gccttatctg gtcgagcagg gattctctcg cggtgattta
    12661 ggtttcgcgc tttcggggat ctcgatcgct tatggatttt cgaaattcat aatgggttcg
    12721 gtatcagatc gctcgaatcc gcgcgttttc ctccccgcag gtttgattct ggcggcggca
    12781 gtgatgttgt ttatgggctt tgtgccatgg gcgacgtcga gcattgcggt gatgtttgta
    12841 cttttgttcc tctgcggttg gttccagggg atggggtggc cgccgtgtgg tcgtactatg
    12901 gtgcactggt ggtcgcagaa agaacgtggc ggcattgtgt cagtgtggaa ctgtgcgcac
    12961 aacgttggtg gtggtattcc gccgctgctg ttcctgctgg ggatggcctg gttcaatgac
    13021 tggcatgcgg cgctctatat gcctgctttc tgcgccattc tggtggcatt attcgccttt
    13081 gcgatgatgc gcgatacccc gcaatcctgt ggcttgccgc cgatcgaaga gtacaaaaat
    13141 gattatccgg acgactataa cgaaaaagcg gaacaggagc tgacggcgaa gcaaatcttc
    13201 atgcagtacg tactgccgaa caaactgctg tggtatatcg ccatcgccaa cgtgttcgtt
    13261 tatctgctgc gttacggcat cctcgactgg tcaccgactt atctgaaaga ggttaagcat
    13321 ttcgcgctgg ataaatcctc ctgggcctac ttcctttatg aatatgcagg tattccgggc
    13381 actctgctgt gcggctggat gtcggataaa gtcttccgtg gcaaccgtgg ggcaaccggc
    13441 gttttcttta tgacactggt gaccatcgcg actatcgttt actggatgaa cccggcaggt
    13501 aacccaaccg tcgatatgat ttgtatgatt gttatcggct tcctgatcta cggtcctgtg
    13561 atgctgatcg gtctgcatgc gctggaactg gcaccgaaaa aagcggcagg tacggcagcg
    13621 ggctttaccg ggctgtttgg ttacctgggc ggttcggtgg cggcgagcgc gattgttggc
    13681 tacaccgtgg acttcttcgg ctgggatggc ggctttatgg taatgattgg cggcagcatt
    13741 ctggcggtta tcttgttgat tgttgtgatg attggcgaaa aacgtcgcca tgaacaatta
    13801 ctacaaaaac gcaacggagg ctaatggcat gaaattgacg ctgaaaaacc ttagcatggc
    13861 gatcatgatg agcactatag tcatgggaag cagtgcaatg gcggcggaca gcaacgaaaa
    13921 aatagtcatc gcccatcgcg gtgccagtgg atatttgccg gagcatacgc tgccagcaaa
    13981 agcgatggcg tatgcgcagg gagcggatta tctggaacag gatttggtga tgaccaaaga
    14041 cgaccatctg gttgttctgc atgaccatta tctcgatcgt gttactgatg ttgccgatcg
    14101 tttcccggat cgggcgcgca aagacggtcg ttactacgcg atagatttca cgctggatga
    14161 aattaagtcg ttgaaattta ccgaaggttt cgatattgaa aacggtaaaa aagtgcagac
    14221 ttatccgggg cgtttcccaa tgggtaagtc cgacttccgg gtgcacacct ttgaagaaga
    14281 gattgaattt gttcaggggt taaatcactc taccgggaaa aatatcggta tttatccaga
    14341 aatcaaagcg ccgtggttcc atcatcagga agggaaggat attgcggcaa aaacgctgga
    14401 agtgctgaag aaatatggtt acaccggtaa agacgataaa gtttatttgc aatgttttga
    14461 tgctgatgag ctgaagcgta ttaagaatga gctggaaccc aaaatgggca tggagctcaa
    14521 tctggtacag ctgattgcct ataccgactg gaatgaaacg cagcagaaac agccggatgg
    14581 aagctgggtt aattacaact acgactggat gtttaagccg ggtgccatga aacaggtggc
    14641 ggaatatgca gatggtattg gtccggatta ccatatgttg attgaggaga catcgcagcc
    14701 gggtaatatc aaactcactg gcatggtgca agatgctcag cagaataaac tggtagtgca
    14761 tccttatacc gtgcggtcag ataaactgcc tgaatacact cctgatgtga atcagttata
    14821 tgatgctctg tataacaaag cgggtgtaaa tgggctgttt actgatttcc ctgataaggc
    14881 agtaaaattt cttaataaag agtaatgtta ctgcaggatg gcgttcatgc gccatcctgt
    14941 ctcatatcaa atttttccaa tcgccactaa ttgttccatg cacatttgta gtaaaaacag
    15001 tggactgcct tctaccggct tttctttagc cagcaacgaa atctgtcgat agaaagttgg
    15061 ttcgagagga acggaacaca attcacctgc aatgcttttc agcgttaact ctggttgtaa
    15121 ggcgatgcct aatccctgtc gaataaaatt catcgctgta tccgcctgat taaattcaca
    15181 ttttatctgc tgggcgcact attagtggta gtggctggtg gaatctatgc gctgcaaatg
    15241 aagggtaaaa gcggtgtcaa gctagtagtg gcaaaagaaa ttcattaata cgacacgttt
    15301 cattaagatt ttcctcaggt aaccacctat agtcattgtc gttaataaaa acgacaggcg
    15361 atagggtatg gcagtttcag caaagtacga cgaatttaat cactggtggg caacagaggg
    15421 cgactgggtt gaagagccta actatcgccg taatggtatg agcggggtgc aatgcgtcga
    15481 gcgcaacggc aaaaagctgt atgtaaagcg catgacgcat catctgtttc attctgtacg
    15541 ttatccgttc ggccgaccaa cgattgtccg tgaggttgcg gtaattaaat aactggaacg
    15601 ggccggtgtc atcgtgccga agatcgtttt tggtgaagcg gtgaaaattg agggtgaatg
    15661 gcgagcgttg ctggtgactg aagatatggc ggggttcatc agcattgctg actggtatgc
    15721 ccagcatgca gtatcgcctt attctgacga agtacggcaa gccatgttga aagcagtggc
    15781 gctggcgttt aagaaaatgc atagcattaa tcgtcagcat ggctgttgtt atgttcgcca
    15841 tatctatgtg aaaacagaag gtaaggcaga agctggtttt cttgacctgg agaaaagtcg
    15901 tcgccgcttg cgtcgcgata aagcgattaa tcatgacttc cgccagctgg aaaaatatct
    15961 ggagccaatc cccaaagcgg actgggagca ggtgaaagcg tattactacg ccatgtaatc
    16021 gttgtaggcc agataagaca cataagcgtt gtatctggca ctacaaccaa ttcacatctc
    16081 gatttcaata tcgccttttg cccggcaaca acagggcaaa atttcccccg gctgaataaa
    16141 ggctaacggt tcggcaatcc agtcaacttg acctgcaacc aggcgtgtgc gacaggagcc
    16201 gcagtaacct tcgcgacact ggtactcaac cgccacattg tgggattcca gcgctgccag
    16261 aagggaaggg tgttcatcct ggcacagcag ttgtgtgcca gtgatgcgca gggtaacgcg
    16321 ggccatcaga gctggaagtt actcaaatcg tcggtgtcca cttccgagtc aatctgcccg
    16381 accagataag aactgacttc cacttcctgc ggagcaacct gcacgttatc agacaccagc
    16441 caagtgttga tccacgggat cgggttggag cgcgtctgga acggcagatc caaaccgact
    16501 gcctgcatac ggatattggt gatgtattca acgtactggc agagaatgtc tttattcaga
    16561 ccaatcatcg aaccgtcgcg gaacagataa tccgcccagt ctttctcctg ttgagctgcc
    16621 tgaacaaaca ggtcatagca ctcctgctta cactcttcgg caatttccgc catctcagga
    16681 tcgtccgcgc cgctgcgcag cagattcagc atatgctggg tgccggtcag gtgcagggct
    16741 tcgtcgcggg caatcaggcg aataattttg gcgttgcctt ccatcaattc gcgttctgca
    16801 aaggcaaagg aacaagcaaa gctgacgtag aagcggatcg cttccagcgc gttaacgctc
    16861 atcaggcaaa gatacagttt tttcttcagc tcgcgcaggc taacggttac ggttttaccg
    16921 ttaacggtgt gggtgccttc gcccagaagg tgccagtagc tggtcatttc gatcagttca
    16981 tcgtaatagc tggagatccc ttccgcacgt ttctggatct gctcgttggt gacgatatcg
    17041 tcaaacacaa cagacggatc gttaacgata ttacgaatga tatgagtata ggaacgggaa
    17101 tgaatcgttt ctgagaacgc ccaggtttcg acccaggttt ccagttccgg aatagaaata
    17161 agcggcaata gcgccacgtt cggactacga ccctgaatgg aatccagcag cgtctgatat
    17221 ttcaggttgc tgataaaaat gtgtttttcg tgctccggca gcgcctggta atcgatacgg
    17281 tcgcgggaga cgtcaacttc ttccggacgc cagaagaaag agagctgctt ttcgatcagc
    17341 ttttcgaaga tgtcatattt ttgctgatcg tagcgggcca cgttgaccgg ctgaccaaag
    17401 aacatcggtt ctttgagctg atcatttttc gtctgtgaaa aggtggtata tgccatgagt
    17461 gtgtcctgtt gggagtttaa tgccggataa ggcattttac gccgcatcct gtgcctgatg
    17521 cgacgctggc gcgtcttatc aggcctacaa atccgagccg taggccggat aaggcgttca
    17581 cgctgcatcc ggcatctcaa tatcagatct tacatgcgcc gctttcgcag ccatcgtcct
    17641 ggattgacgg taccagatcg tcctgcgcgt cttcagcgcc gtcacgggtg ttctgataat
    17701 acagcgtttt gaccccgaat ttgtaggcgg tgagcaggtc tttcagcaac tgctgcatcg
    17761 gcacttttcc tgacgggaaa cgtgacggat cgtagttggt gttagcagag atcgactgat
    17821 cgataaattt ctgcatgata cctaccagtt gcaggtagcc atcgttaccc ggcatttccc
    17881 acagcagctc ataggcgtcg tgcaggtgct cgtagtccgg caccacctgg cgcaaaatac
    17941 cgtctttcga cgctttgatg ctgacgtaac cgcgcggcgg ttcaataccg ttagtggcgt
    18001 tagagatctg cgaagaggtc tcggacggca tcagggcaga aagcgtggag ttacgcaggc
    18061 cgtgcgtttt gattgattca cgcaatgctt cccagtcgta atgcagcggc tcattagcga
    18121 tggcatccag atctttctta taggtatcga tcggcaggat ccctttcgcg taagtggttt
    18181 cgttaaacca cgggcactcg ccttgctctt tagccagctc attagacgct ttcagcaggt
    18241 aatactgaat cgcttcgaag gttttatgcg tcaggttgtt ggcgctgccg tcggagtagc
    18301 gtttaccgtg cttcgccagg tagtaagcga agttgatcac accaataccc agcgtacgac
    18361 gacccatcgc tccacgtttg gcggccggga tcgggtaatc ctgataatcc agcagcgcgt
    18421 caagtgcacg aaccgccaga attgccagct cttccagttc atccaggtta ttaattgcgc
    18481 ctaggttgaa agcagacagc gtacacagcg cgatttcacc gttctcgtcg ttgacgtcgt
    18541 tcagcggttt ggtcggcagg gctatctcca ggcacaggtt agactgacgc actggcgcga
    18601 tggccggatc aaacgggcta tgggtattac agtggtcaac gttctgaata tagatacgac
    18661 cggtagacgc acgttcctgc atcatcagcg agaacagctc aacggctttc acacgctgct
    18721 tgcggatgct gtcgtctttc tcatatttgg tatacagacg ttcaaactct tcctgatcgg
    18781 cgaagaacgc gtcgtacagc cccggtacgt cggacgggct gaacagggtg atatcttcac
    18841 ctttcagcag acgggtatac atcagtttgt tgatttgtac cccgtagtcc atatgacgca
    18901 cgcggttgcc ttccacacca cggttgtttt tcaacaccag caggctttcc acttccagat
    18961 gccacatcgg gtagaacagc gttgccgcac cgccgcgcac accgccctga gagcaggatt
    19021 tcaccgctgt ctggaaatgt ttgtagaacg gaatgcagcc ggtatggaac gcttcaccac
    19081 cgcgaatcgg gctacccagc gcacgaatac gcccggcgtt gatgccgatc ccggcacgct
    19141 gggaaacgta tttaacaatc gcgctggagg tggcgttgat ggaatccagg ctgtcaccgc
    19201 actcgatcag tacgcaggag ctgaactgac gagtcggggt acgcacgccg gacatgattg
    19261 gcgtcggcag cgaaatttta aatgtggaaa ccgcgtcgta aaaacgcttc acatattgca
    19321 ggcgcgtttc acgcgggtag ttcgagaaca ggcacgcggc aaccagaata taaaggaact
    19381 gggcgctctc atagatttcg ccggtcacgc ggttctgtac cagatatttg ccttccagct
    19441 gcttaacggc agcataagag aaggtcatat cacggtcgtg atcgataaac gtgtccatct
    19501 gcttgaactc ttcttccgtg tagtcttcca gcagatgatt atcgtatttg cccatctcga
    19561 ccattttcac cacgtggtcg tacagcgcag gcggctcaaa ctggccgtag gcttttttac
    19621 gcaggtggaa gatcgccagg cgcgcggcga gatactgata atccggcgca tcacgggaga
    19681 tcaggtctgc ggcagccttg ataatggttt cgtggatgtc agaggtcttg ataccgtcat
    19741 aaaactgaat gtgggagcgc agctcgacct gggaaatcga aacgttatgc agtccttctg
    19801 ccgcccaatc cagaacgcga tggattttgt cgagattgat gcgctctgtg ctaccgtcgc
    19861 gctttgtcac cagcagattc tgattcatgt atgtcgtacc tgtttttgga attctttccg
    19921 caatagtttc atgcagacta cctgtagtac gggaagctgc ttagaacact atatataggg
    19981 ggtatgtttg ggattcactg caagatagtg tgaaaatggc cctcttgcaa gtgcataact
    20041 ttgtggataa ctcaggaagg aaaaagtggc tttcgcgcac cttaggtcag acaaggtgtc
    20101 cgggaaagtc aatgggaaga gaaaaatttg ttaaaaataa ctgttcggcg atttcttgaa
    20161 cggcagaata ggttggataa attgatatag atggtttatt aatctttttg ctaatgattg
    20221 caaaaattga tctaagcacg agtcatcaca taatctgatg tatcagttgt ggtcaatata
    20281 taagcgaatg taagcgattg ttgatgtcga aatggttgtt ttctattcgg ttattgttaa
    20341 atgcgaagcc attttgttaa tttaccttat attataaaaa aattattcat tactttaatt
    20401 tagtttaaat ataactggta tcccttaagg ttattaactt gaattatgac gcactgatat
    20461 tattcatcaa ataataacaa aatagccatt gcatcgggtt ggacaattta caaaagaaag
    20521 ataacccata ttgtataatg cgtttaaata tgccgtctta tataaaatga tggtcagatt
    20581 aatatgcgga ttatctttct acg
//
LOCUS       DADLFA010000062        20594 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000062 DADLFA010000000
VERSION     DADLFA010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20594
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.062"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..251)
                     /locus_tag="JQH65_001898"
     CDS             complement(<1..251)
                     /locus_tag="JQH65_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAY6139327.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARS
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKR"
     gene            complement(344..991)
                     /gene="rnt"
                     /locus_tag="JQH65_001899"
     CDS             complement(344..991)
                     /gene="rnt"
                     /locus_tag="JQH65_001899"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HAY6139328.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMIAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(1094..1501)
                     /gene="gloA"
                     /locus_tag="JQH65_001900"
     CDS             complement(1094..1501)
                     /gene="gloA"
                     /locus_tag="JQH65_001900"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAY6139329.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFMEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(1582..2679)
                     /gene="nemA"
                     /locus_tag="JQH65_001901"
                     /pseudo
     CDS             complement(1582..2679)
                     /gene="nemA"
                     /locus_tag="JQH65_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
     gene            complement(2716..3315)
                     /locus_tag="JQH65_001902"
     CDS             complement(2716..3315)
                     /locus_tag="JQH65_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY6139330.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYTAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            3418..3657
                     /locus_tag="JQH65_001903"
     CDS             3418..3657
                     /locus_tag="JQH65_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HAY6139331.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            3706..4598
                     /locus_tag="JQH65_001904"
                     /pseudo
     CDS             3706..4598
                     /locus_tag="JQH65_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
     gene            4679..5200
                     /gene="sodC"
                     /locus_tag="JQH65_001905"
     CDS             4679..5200
                     /gene="sodC"
                     /locus_tag="JQH65_001905"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HAY6139332.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVITPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(5201..6809)
                     /locus_tag="JQH65_001906"
                     /pseudo
     CDS             complement(5201..6809)
                     /locus_tag="JQH65_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016165.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(6890..7199)
                     /locus_tag="JQH65_001907"
                     /pseudo
     CDS             complement(6890..7199)
                     /locus_tag="JQH65_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378749.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7213..8070)
                     /locus_tag="JQH65_001908"
     CDS             complement(7213..8070)
                     /locus_tag="JQH65_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY6139333.1"
                     /translation="MSIKTIKYFSIIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEK
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LRGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(8136..8309)
                     /locus_tag="JQH65_001909"
     CDS             complement(8136..8309)
                     /locus_tag="JQH65_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HAY6139334.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPL"
     gene            8510..8944
                     /gene="slyA"
                     /locus_tag="JQH65_001910"
     CDS             8510..8944
                     /gene="slyA"
                     /locus_tag="JQH65_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HAY6139335.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(8991..9458)
                     /gene="slyB"
                     /locus_tag="JQH65_001911"
     CDS             complement(8991..9458)
                     /gene="slyB"
                     /locus_tag="JQH65_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HAY6139336.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            9732..10841
                     /gene="anmK"
                     /locus_tag="JQH65_001912"
     CDS             9732..10841
                     /gene="anmK"
                     /locus_tag="JQH65_001912"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HAY6139337.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            10939..11268
                     /gene="mliC"
                     /locus_tag="JQH65_001913"
     CDS             10939..11268
                     /gene="mliC"
                     /locus_tag="JQH65_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HAY6139338.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            11327..11983
                     /gene="pdxH"
                     /locus_tag="JQH65_001914"
     CDS             11327..11983
                     /gene="pdxH"
                     /locus_tag="JQH65_001914"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HAY6139339.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            12112..13386
                     /gene="tyrS"
                     /locus_tag="JQH65_001915"
     CDS             12112..13386
                     /gene="tyrS"
                     /locus_tag="JQH65_001915"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAY6139340.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            13448..14308
                     /gene="pdxY"
                     /locus_tag="JQH65_001916"
     CDS             13448..14308
                     /gene="pdxY"
                     /locus_tag="JQH65_001916"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HAY6139341.1"
                     /translation="MKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNHT
                     QYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKAA
                     NPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNNV
                     EEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQP
                     VGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIAK
                     PEHYFSATKL"
     gene            complement(14352..14957)
                     /gene="gstA"
                     /locus_tag="JQH65_001917"
     CDS             complement(14352..14957)
                     /gene="gstA"
                     /locus_tag="JQH65_001917"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HAY6139342.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNETLKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(15063..16565)
                     /gene="dtpA"
                     /locus_tag="JQH65_001918"
     CDS             complement(15063..16565)
                     /gene="dtpA"
                     /locus_tag="JQH65_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HAY6139343.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(17177..17812)
                     /gene="nth"
                     /locus_tag="JQH65_001919"
     CDS             complement(17177..17812)
                     /gene="nth"
                     /locus_tag="JQH65_001919"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAY6139344.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(17812..18507)
                     /locus_tag="JQH65_001920"
     CDS             complement(17812..18507)
                     /locus_tag="JQH65_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HAY6139345.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(18511..19131)
                     /gene="rsxG"
                     /locus_tag="JQH65_001921"
     CDS             complement(18511..19131)
                     /gene="rsxG"
                     /locus_tag="JQH65_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HAY6139346.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(19135..20193)
                     /gene="rsxD"
                     /locus_tag="JQH65_001922"
     CDS             complement(19135..20193)
                     /gene="rsxD"
                     /locus_tag="JQH65_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HAY6139347.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSTLLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGADMNTLHLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(20194..>20594)
                     /locus_tag="JQH65_001923"
     CDS             complement(20194..>20594)
                     /locus_tag="JQH65_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="part of membrane-bound complex thought to be
                     involved in electron transport to nitrogen; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HAY6139348.1"
                     /translation="NAEPEEQIDPRKAAIEAAIARAKARKLEQQQANAEPEEQVDPRK
                     AAVEAAIARAKARKLEQQQANAVPEEQVDPRKAAVEAAIARAKARKLEQQQTNAEPEE
                     QVDPRKAAVAAAIARAQAKKAAQQKVVNED"
BASE COUNT         5187 a         5186 c         5310 g         4911 t
ORIGIN      
        1 ttacgcttat gcgcctcgcg ggtatcttcg ccgccttcgc ggaagagccg atcgagggcg
       61 tagagaaatg ctgccagcgt tttcccggag ccggtcggtg caatcaccag cgcatgttcg
      121 cttcgcgccg ccacatgcca ggtttgcgac tggacagcgg tcggctgttt aaaggcgcga
      181 agaaaccagt cgcgggtcgc cggtgaaaac acgtccggca ggagcgatga agggtctgga
      241 ttatctgcca ttaacgctat tccccgtgaa tatcatgcag gatgcgtgga atagcctcac
      301 tatagatgcc tgaatcattg catgtcatca ggcatcgact cgattacacc tcttcggcgg
      361 caggtagcgg ccagcctccc agacgtttcc agcggttgac gatttcacaa aacagcacag
      421 cagtgcgttc ggtgtcgtac agcgcggagt gcgcctgggt gctgtcgaag tccatgccag
      481 cggtctggca agcctttgac aatacggttt gtccgagtgc cagcccggcc agtgcagcag
      541 tgtcaaaagt ggcgaaaggg tggaacgggt tacgtttcag tgaggcgcgt tctgcggcgg
      601 ctatcataaa gctgtgatca aaattggcat tgtgcgccac cataatggcg cggttacagc
      661 cgctcgcttt aataccttta cgtacaactt taaaaatttc gtgcagcgcc tcgtattcgc
      721 tgaccgcgcc acgatcggga tcgttcgggt caatgccgtt gaaggcgagg gcttctggtt
      781 gcaaatttgc gccgacaaat ggttcgacgt ggaaatgtaa tgtggtgtcc ggcatcagcc
      841 agccttgttc atccattttc agggtgatgg cggcaatctc aagcagcgca tcggttttgg
      901 cgttaaatcc ggctgtttca acatcgatca caacaggata aaaaccacga aaacggtcgc
      961 acagaccggt aagttgagcg ttatcggaca tctgattctc ttaatacgga taaattgcag
     1021 cgcgcattat gacaaatatt acttgtcgat gcagtaaaga tgcgggcgcg atgagttcac
     1081 gcccggcagg agattagttg cccagaccgc gaccggcgtc tttctcttcg attaactcaa
     1141 ttttgtaacc gtccggatct tccataaacg cgataaccgt agtaccgcct tttaccggac
     1201 ccgcttcacg ggtcacgtta cccccgtttt gacggatttt ttcgcacgct tcagcggcgt
     1261 tatctacgct aagcgcgatg tgaccataag cagtgccgag ttcgtattta tccacgcccc
     1321 agttgtaggt cagttcaatc accgcttctt cggtttccgg gccgtagcca acaaacgcca
     1381 gtgagtattt gtattccggg ttttcgctgg tacgcagcag tttcatgccc agcactttgg
     1441 tataaaaatc gatggagcgt tgcaaatcgc caacgcgcag catggtatga agaagacgca
     1501 tctttttatc ctcaaaatgg ctaacagatt caaaatgttg ttttagtata gcggcggctt
     1561 tacgccgctc gcaatgttgg attacaacgt cgggtaatcg gtatagcctt ccgcgccgcc
     1621 accgtagaaa ctttcggcac gctgtgggtt aagctcagct ttgcgctgca agcgggcgac
     1681 cagatccggg ttcgcaatcc agtcacgacc aaatgccacc gcatcaatta accctttgcc
     1741 gatcagcgtt tcagcttttt ctactgtgta tgcacctgcg ccgataatcg gaccgtggaa
     1801 acgggcgcgt actttttcgc ggaacgcatc agtatacggt tcaccccccg cccaatctgg
     1861 ttctgacata tgcagataag caatgccgcg tttacccagt tgttcaatca gatacagtgc
     1921 atcggcttct tcattcgggc cgttatccgt gttctggaaa gtaccgattg gcgaaatgcg
     1981 aatgccaatg cgatcggcac cccattcttc aatcccggca tcgaccactt ccagtaccag
     2041 acgtgcgcga ttttccacgc tgccgccgta ctgatcggta cgatggtttg aagaaggaga
     2101 aaggaactga tgcagcaaat aaccgtgagc agagaggagc tctaccagat caaaaccggc
     2161 ttcacgcgcg ttagcaatgg cctgacggaa atcattgacg atacctggaa tctcttctag
     2221 ttcaagcgca cgcggcatgg atgtttcaac acggatcgcc tgaccatttt catcgcgcag
     2281 agaagtacgt gttcccgcgc taagtgctga aggcgctacc ggtgcctgac cgccaggttg
     2341 caggctggcg tgagaaatgc gtccggtgtg ccacagctac acggccatat gaccattttc
     2401 agcatgaacg ccagcggtga tttttttcca tgcggcaatt tgctccggac tatggatgcc
     2461 aggcgcacct gcatatcctt ttacctgggc agaaatttgc gtggcttcac taataatcaa
     2521 accggcactg gcacgttggc gatagtattc cgccatcaac ggggtaggaa tgtcacccgg
     2581 ttcaatactg cgcagacgcg tcagcggtgc cataaaaata cggtttgccg ccgtgatcgc
     2641 gcccactttc agtggggaat acagtttttc agatgacata aaggctcccg gtcgtctggt
     2701 gataaaaaat gcctgctaaa cggcaggcgt cgcaataatg ttttttacat gggccagcgc
     2761 gttttccagt ggctcgaaac tgcgcgaaat tttggcctgc agattcgcac ccagccatag
     2821 tgcgtaaagc acttgtgcct gttgtagcgg ttcgccacaa aaggttaaac aatggttctc
     2881 acggccattt tccagcgcct gagagagcag ggcgatcacg ccgcgagcac ctttatccat
     2941 cgcgctgcgc atatcttctg acagatcgca cacttcggca gagagtttta ctgtcaggca
     3001 accactgatg gttccatgtt ggcaaaactg gttcagtgtt tgctggtaat aagccagtat
     3061 gcggtcgcgg tagttacctt cgccggattg cagcaactca gtcagtcgct ggtgatatgc
     3121 ggtgtaatga cgctcaagca tggcaacgcc aaacgcttct ttagagcgaa agtagtgata
     3181 gaaggaccct ttcggcactt cagcggtttt tagtaattcg cttagcccca tcccggtgaa
     3241 tccacgttgc aggcaaagtt gctcgcccgt cgccaggaga tgttcgcgag tatcatgttc
     3301 ggtgtgtttg ttcatgcgtt ggagtgtagt agaccagtcg gtctagtgca agcaaagttg
     3361 ctactttgga cattaacagc ttcaataatg gtaataatct tatctgagga gggcgaagtg
     3421 gcggagcaat tagagttctt tcccgtccag agcccgtgcc ggggaatttg ccagtctgac
     3481 gaacgcgggt tttgtcgcgg ctgttttcgt agtcgggatg agcgttttaa ctggaataaa
     3541 atgagcgatg gcgaaaaaca agaggtgctg cgcctttgtc ggcagcgatt aatgcgcaaa
     3601 ttacgcgcaa ataagccagc gtcatcagac gaaccggagc aaccatcact cttttgaacc
     3661 taatcttgcg tatactcctc tcatatctga taagaggaag cgattatggt tcagcgtatt
     3721 actattgcgc cgcaaggccc ggagttttcc cgttttgtga tgggctactg gcgattgatg
     3781 gactggaata tgtccgcccg ccagctggtc agttttattg aagagcatct ggatctcggc
     3841 gtgaccaccg tggaccatgc tgatatttat ggtggctatc agtgcgaagc ggcgtttggc
     3901 gaggcactga aactggcacc tcacctgcgt gaacggatgg aaatcgtcag taaatgcggt
     3961 atcgcgacga ccgcgcgtga agaaaacgtc attggtcatt acatcactga ccgcgatcac
     4021 atcattaaga gcgccgaaca gtcgctaatt aatctcgcga ccgatcatct ggatctgctg
     4081 ttaatccacc gaccagaccc gttaatggat gccgatgaag tggcggacgc gttcaaacat
     4141 ctgcatcaga gcggcaaagt gcgtcatttt ggcgtatcga actttacgcc tgcgcaattt
     4201 gccctgttgc aatcacgtct gccgtttacc cttgccacta atcaggtgga aatatccccg
     4261 gtgcatcagc cgttactgct ggatggcacg ctcgaccaac tacaacaact gcgtgttcgt
     4321 ccgatggcgt ggtcctgcct tggtggtggt cgtctgttta atgatgatta tttccagccg
     4381 ctgcgtgatg aactggctgt ggtggcagag gagttaaacg cgggctcgat tgaacaggtg
     4441 gtttctgggt attacgttta ccatcgcagc cgctgccaat tatcggttca ggtaaaattg
     4501 agcgcgtacg ggcagctgtc gaagcagaaa cactgaaaat gacccgtcaa caatggtttc
     4561 gtattcgtaa agcggcactg gggtacgacg taccgtaagc acttttagga atagccgccg
     4621 ttcaaaaatg tgtcactggt ttacacttat tcaggaatgc acaatgaacg gaggtcctat
     4681 gaaacgtttt agtctggcta ttctggcgct ggttgttgca accggcgcac aagctgccag
     4741 tgaaaaagtc gagatgaacc tcgtcacgtc gcaaggggta gggcagtcaa ttggtagcgt
     4801 caccattact gaaaccgata aaggtctgga gttttcgccc gatctgaaag cattaccccc
     4861 cggtgaacat ggcttccata ttcatgccaa aggaagctgc cagccagcca ccaaagatgg
     4921 caaagccagc gccgcggaat ccgcaggcgg gcatcttgat ccacaaaata ccggtaaaca
     4981 tgaagggcca gaaggtgccg ggcatttagg cgatctgcct gcactggtcg tcaataatga
     5041 cggcaaagct accgatgccg tcatcacgcc tcgtctgaaa tcactggatg aaatcaaaga
     5101 caaagcgctg atggtccacg ttggcggcga taatatgtcc gatcaaccta aaccgctggg
     5161 cggtggcggt gaacgctatg cctgtggtgt aattaagtaa ttaagaggcc tgcgtacctt
     5221 gcggtggtgc ttgctcaagt tgcgaaagcg agcagtacag ccgccagaca attgccgcca
     5281 gctcacgggc ggcaggttga tgatgacggg caaggctgtc gcaaatccgc tgtaattctt
     5341 caagtgtggc cgccagtgat ttttgctgaa cgccacgctc actcatcact ccccgcaaca
     5401 gtgaaataca gttatcccgt actcgcgata acggatcgga acgcgattcc cagtcgcgca
     5461 attgccagac aacatgagaa cagttcagca gcactacacc ccagcgtaat aaccaacggc
     5521 gagccagcgc atcctggctg ttactcaact gactgacgtg atgataagtg agcgattcaa
     5581 attcgctttc actcagtgtt ggatggcggc ttagctgatc gacaaaatcc cggcgcagcg
     5641 cgcgaatatg gcggcggctt ttacgagcat ccgatcctgg acgcagaatg gcgaacgcta
     5701 gccaagccag cgcaacgcca acgatttttg ccagattatc gttaagaaag tcagcaaaat
     5761 catacaccgg gggattagtg acagcgataa acgaacccat aaaaacaatc agttgccccc
     5821 acagtgcggc aaatttgggc atctgcaatt taagaagctg cattgtcgcc agcagtggaa
     5881 agagaaacag taaaaattgc cacagatcgc taatctggac catcaggcca aatttgacca
     5941 caaagctgaa tagcgaaagt aacaccagcg tgcgcatcag cagtgacaac gacttaaacg
     6001 gtgctgcgac ggcggagtag agtacgcagc taattgctgc cagcgttaat gcattggcac
     6061 cggcatccta ttgcgatgca atactccatg cgccaatcat catcaacgta caaaatgtac
     6121 gcagcccgct ccacatagct tcggcgttgt cggtatgacg agccagagcg gatgtccgtg
     6181 cgagtctggc atgatcatct acaccgcttt gcagacgatg taattcctga ctactttgca
     6241 gataaaggcg gcaaaaataa cgtagtcgct gccagaaggc gacgtcgccg gtagtcggcg
     6301 acgttggtcg ggcgtagcgg ggcgataata cgtgcgacgg tgtaagcatc tgtttgcgaa
     6361 ctggcgagcg ccgtcagcaa ttgttcgaga atttctcgcg tggcacctgg cggtgagggc
     6421 cagttgagca acatacgtcg caggctggag atgacactgg tcatacgtaa ttgctggtgg
     6481 agtagcgcat taaggcgcgc gttttgctgg cgaaaacgat agtggctcca gaaagcctgg
     6541 atacgcagca aattcatggt cagtatctgc ccaatccccc cctcatgtgc tgcacgaatg
     6601 gcatcgtttg tttcaggctg ccagagtaaa ctggcatgtt ccagtaatcg ggcgtgcatg
     6661 tttttcaatg cggttaaaag ggctgtagca tcggaactgc taggcaggat catcatcatc
     6721 atgccgccgc acaaaatacc gactatcacc tcgcaaacgc gcgcctgagc gatatcctac
     6781 agctggctgg cctcagtaat attaaccatc ggaaaggcga tgatggcagc cgtgtagccg
     6841 ccagttgaaa tgcatacgcg acgttattcg tgaagtgcgc acaggcccag gtacaaaagc
     6901 caagccacgc cgacatgctc aatagaaaaa accacggctc attgagcgta tgcccggcaa
     6961 gaagcagtgc cgcaatggct cccagtaaac tgccagcgat gcgtccgagg cttttgctga
     7021 tgacaccgcc aacggtggga aagctaacca ctgcagccga ggtcatcgcc caatagggtt
     7081 catccagatt caaataatag gcaaccgtca gcgccagaca catggcaatg gtattgcgta
     7141 acgcataacg ccattgcgcc agcgtggcct tgaaccaggg caaattgcgc aaggaccatg
     7201 acgatgcgtt cattattgac ctacggcaat gctacaggtg gtgcccgaca ccagcgtgac
     7261 atcgcctgga actttatcca gcgcaaagcg gacgggaacg cgttgggcga gacgaaccca
     7321 ggggacgtta ggtttcacat ccggaataag gcttgagtca ctttcaacgc tttgatcata
     7381 aatcgcccga ccgatactgg aaacgtgacc ccgtaacgtt ttgttatcac tatagagggt
     7441 aatttgtgct ggtgcacctt cgcggatatg gcgcaacttg gtttcttcaa aataaccaat
     7501 gacgtaaaac gagtggctgt cgacaagggc aaacagcggt ttcccggtgt cagcgtaatc
     7561 gccgatgcgg gtagtgaggt tagtcaccca accactcacc ggggcgcgaa tttccgtttg
     7621 cgccagttgc cattgtgtct gtttcagcgt ggcctgtgcg gcattaacac tggcctgcat
     7681 cgctttaaca ttgaggttag cggtatcgag ttcttcggca gaaataaaat tttgtgagag
     7741 atgacgacga cgattcgctt cgttattggc ttttgcgagg tcagattgcg ctttcgccag
     7801 ctgagcctgt gcgttcagct cggcgatctg aaagggcgtt ttatcgatcg tgagcagaag
     7861 atcccccgcg ttaaccagtt ggttgtcttt tatattcagc tcaacaatac gtcccgacac
     7921 ctgtggggtg atagaaacct tttctgcgcg tattttcccg tcgcgtgtcc acggagattg
     7981 catgtaataa ttccacagcc accatccggc aagaaccgca actatcgcta caatgattat
     8041 tgagaaatac ttaattgttt taattgacat agttaccacg caatcagtat tgccagagca
     8101 aggcaaacgc aaatcgcaaa cagcgataaa tccatttaca agggatgcca gatgtcaccg
     8161 gcgtagatcc agccacgaag caggcggtgt acgacaagcc agatgacgaa tccaaacgcg
     8221 aaggctttga aaaacggagg aaaatagacg gacgcaccga acaccagatc ttgtaagggc
     8281 aatcctgttg cattgagcat aaacttcaca aagtagattc ctttacgacc gaatatattg
     8341 cgttagatta ataaatattc tttaagtgcg aaaaatttac gcgcaatttc tgaaaaatat
     8401 gtctgacggt aaccaaatgc agcaatacat ttgttttagc aataaaattg ctgcacacta
     8461 ttctaaaagc cgcataatat cttagcaagc taattataag gagatgaaat tggaatcgcc
     8521 actaggttct gatctggcac ggttggtgcg catatggcgt gctctgatag accatcgcct
     8581 gaaaccgctg gagttaacac aaacccattg ggttacgtta cacaatatcc atcagttacc
     8641 tccagaccag tcgcaaattc aactggcaaa agcgattggc atcgagcagc catcactggt
     8701 ccgtactctg gaccaactgg aagaaaaagg gttaatttcg cgtcaaactt gtgccagcga
     8761 tcgtcgggct aaacgtatta aactgacgga aaaggcagag ccactgatca gcgaaatgga
     8821 agctgttatt aacaaaaccc gcgcggaaat attacatggc atctccgcag aggaactgga
     8881 gcaactgatt acgctcatcg caaaacttga gcataatatc attgagttac aggccaaagg
     8941 gtgaaatgaa gggggcgtgt ggtcagtaac ctgaccacac gcaaacttat ttagcgcgga
     9001 gaaacggtca cctgactgcc attgctggcc agtacgacac gttggcccgg agagaaacga
     9061 gtgttgcctt gtttctgtac caccatgatg gtattaccat cgtctttacg aatttccagc
     9121 tcgacaccct gcgttttgtt cattgcactc tgtacgccct gaccagctac gccacctgca
     9181 acagcgcctg ctgcagtagc cagagaacgc ccggttccgc caccaacagt attccccagg
     9241 aaaccaccaa gaacagcacc gccaattgca ccgataacgt tggaatcatc accgccctga
     9301 atctgtaccg gacgtacgtt aacgatggtg ccatagctga cattctgtac ttgtttcgct
     9361 tcagaagcgg tataaacatc ccctgacagg gtgtcgttat taacacaacc gacaagagac
     9421 agacctacca ttgaaacaac caatacgcgt ttaatcattg aaaaatctcc tgttcaccat
     9481 gaaacgccac gcaagcatcc ctcatggtca aagtatatgg catatgtgaa tccatggtta
     9541 catgttctac caaatatgag caaatcataa tgaaagtagg atcaaccaat tataaacaaa
     9601 acattcatga tgattattga tttgaagata atgttttact accaacggta aaaatcggtc
     9661 attccgcatg gtattcacga aaagtttatg tttgagtggt gcgcaaacgt cagattgaaa
     9721 aaggatgaac tatgaaatcg ggccgcttta ttggcgttat gtcaggcacc agccttgatg
     9781 gtgttgatgt tgtgttggcg acaatagatg aacaccgggt cgcacagctg gcaagcttga
     9841 gctggccgat cccggtgtct ctgaaacagg ctgtactgga tatttgccag ggccagcagc
     9901 ttacactttc gcagtttgga cagcttgata ctcaactcgg gcgacttttt gctgatgcgg
     9961 tcaatgcctt gcttaaggaa caaaacctgc aggcgagaga tatagttgcg atcggctgtc
    10021 acggtcaaac cgtctggcat gaaccgacgg gcgtggcacc acacacttta cagattggcg
    10081 ataacaatca aattgtggca cgcaccggaa ttacggttgt cggtgatttt cgccgtcgcg
    10141 atattgcctt gggaggacaa ggcgcaccgc tggtacctgc gttccatcat gccttgctgg
    10201 ctcacccaac cgagcgacga atggtgctca atattggcgg catcgccaat ctgtcactgc
    10261 tcattcctgg gcagccggtt ggcggctacg ataccggtcc tggtaacatg ctgatggatg
    10321 cctggatctg gcgtcaggcc ggtaaacctt acgataaaga tgccgagtgg gcacgggcgg
    10381 gtaaagttat tctcccactg ctgcaaaata tgctcagcga cccatatttc tcgcaacctg
    10441 caccgaaaag caccggacgc gaatacttta actacggctg gctggagcgc catttacgcc
    10501 attttccggg tgttgatccc cgtgatgttc aggccacact ggcagaactc accgccgtga
    10561 ccatttctga acaagttttg ttgagcggtg gctgcgaacg attgatggta tgtggtggag
    10621 gtagtcgtaa tccgctactc atggcgcgtc tggcggcatt actgccaggc acagaagtca
    10681 ccaccaccga tgccgttggc attagtggcg atgacatgga agcattggct ttcgcctggc
    10741 ttgcctggcg gacgctggcg ggattaccag gaaatctgcc ttccgtcact ggcgcaagcc
    10801 aggagacggt actgggggct attttccctg ctaacccgtg atatcagagt taactgaatt
    10861 tttcttaccg cgtctgccgt tacacttgga acgttaggga gggcgtattg ccctccagac
    10921 caggaaagtc ttcgggaaat gtctatgaaa aaactgttac tgatctgttt gccggtgctt
    10981 ctcaccggct gtagcgcgtt caatcaactg gttgagcaca tgcaaaccgc tacgctggaa
    11041 taccagtgtg atgaaaaacc gttgacggtc aaactgaata atccgcgcca ggaagtcagt
    11101 tttgtttacg ataatcaact actgcatctc aaacagggca tttcagcgtc tggtgcacgt
    11161 tacactgacg gaatctatgt tttctggtcg aaaggcgatg aagcgactgt ctataaacgc
    11221 gaccgcatcg tattgaataa ctgtcagtta caaaatccac agcgttgaga tttttccagg
    11281 ggcggcgcac aatagcgtca cccactgaca atccgtaaag aaaaccatgt ctgataacga
    11341 cgaattgcag caaatcgcgc atctgcgccg tgaatacacc aaaggcgggt tacgccgtcg
    11401 cgatcttccc gccgatccat taaccctttt tgaacgttgg ctctctcagg cgtgtgaagc
    11461 caaactggca gaccctaccg cgatggtggt cgctaccgtg gatgaacatg ctcagcctta
    11521 tcaacgcatc gttttactca aacattacga cgaaaaaggc atggtgtttt acaccaacct
    11581 cggtagccgt aaagcacatc aaattgaaaa taatccgcgc gttagcctgc tgttcccgtg
    11641 gcataccctt gaacgccagg tgatggtgat cggtaaagca gaacgacttt cgactctcga
    11701 agtgatgaaa tattttcata gccgcccgcg tgatagccag attggtgcat gggtttcgaa
    11761 gcagtccagt cgcatttctg cccgcggtat ccttgaaagt aaattcctgg agctgaagca
    11821 gaagtttcaa cagggcgaag tgccattgcc gagcttttgg ggcggttttc gcgtcagcct
    11881 tgaacagatt gagttctggc agggcggtga gcatcgcctg catgaccgct ttttgtacca
    11941 gcgagaaaat gatgcgtgga agattgatcg tcttgcaccc tgaaaagatg caaaaatctt
    12001 gctttaatcg ctggtactcc tgattctggc actttattct atgtctcttt cgcatctggc
    12061 gaaaagtcgt gtaccggcaa aggtgcagtc gttatataca tggagatttt gatggcaagc
    12121 agtaacttga ttaaacaatt gcaagagcgg gggctggtag cccaggtgac ggacgaggaa
    12181 gcgttagcag agcgactggc gcaaggcccg atcgcgctct attgcggctt cgatcctacc
    12241 gctgacagct tgcatttggg gcatcttgtt ccattgttat gcctgaaacg cttccaacag
    12301 gcgggccaca agccggttgc gctggtaggc ggcgcgacgg gtctgattgg cgacccgagc
    12361 ttcaaagctg ccgagcgtaa gctgaacacc gaagaaactg ttcaggagtg ggtggacaaa
    12421 atccgtaagc aggttgcccc gttcctcgat ttcgactgtg gagaaaactc tgctatcgcg
    12481 gcgaacaact atgactggtt cggcaatatg aatgtgctga ccttcctgcg cgatattggc
    12541 aaacacttct ccgttaacca gatgatcaac aaagaagcgg ttaagcagcg tctcaaccgt
    12601 gaagatcagg ggatttcgtt cactgagttt tcctacaacc tgttgcaggg ttatgacttc
    12661 gcctgtctga acaaacagta cggtgtggtg ctgcaaattg gtggttctga ccagtggggt
    12721 aacatcactt ctggtatcga cctgacccgt cgtctgcatc agaatcaggt gtttggcctg
    12781 accgttccgc tgatcactaa agcagatggc accaaatttg gtaaaactga aggcggcgca
    12841 gtctggttgg atccgaagaa aaccagcccg tacaaattct accagttctg gatcaacact
    12901 gcggatgccg acgtttaccg cttcctgaag ttcttcacct ttatgagcat tgaagagatc
    12961 aacgccctgg aagaagaaga taaaaacagc ggtaaagcac cgcgcgccca gtatgtactg
    13021 gcggagcagg tgactcgtct ggttcacggt gaagaaggtt tacaggcggc aaaacgtatt
    13081 accgaatgcc tgttcagcgg ttctttgagt gcgctgagtg aagcggactt cgaacagctg
    13141 gcgcaggacg gcgtaccgat ggttgagatg gaaaagggcg cagacctgat gcaggcgctg
    13201 gtcgattctg aactgcaacc ttcccgtggt caggcgcgta aaactatcgc ctccaatgcc
    13261 atcaccatta acggtgaaaa acagtccgat cctgaatact tctttaaaga agaagatcgt
    13321 ctgtttggtc gttttacctt actgcgtcgc ggcaaaaaga attactgtct gatttgctgg
    13381 aaataatgca ttaagtggaa agggggagtg agaaatcact ccccctggtt tttatacagg
    13441 gaacatgatg aaaaatattc tcgctatcca gtctcacgtt gtttatggtc atgcgggtaa
    13501 cagtgcggca gagtttccta tgcgccgcct gggcgcgaac gtctggccgc tgaacaccgt
    13561 tcaattttct aatcacaccc agtacggcaa atggactggc tgcgtgatgc cgcccagcca
    13621 tttaaccgaa attgtgcaag gcattgccgc cattgataaa ttacacacct gtgatgccgt
    13681 gttaagtggc tatctgggat cggcggagca gggtgaacat atcctcggta tcgtccgtca
    13741 ggtgaaagcc gcgaatccgc aggcgaaata tttttgcgat ccggtaatgg gtcatccgga
    13801 aaaaggctgt atcgttgcac cgggtgtcgc agagtttcat gtgcggcacg gtttgcctgc
    13861 cagcgatatc attgcgccga atctggttga gctggaaata ctctgtgagc atgcggtaaa
    13921 taacgtcgaa gaagcggttc tggcagcgcg cgaactcatt gcgcaagggc cacaaattgt
    13981 gttggttaaa cacttggcgc gagctggcta cagccgtgac cgttttgaaa tgctgctggt
    14041 caccgccgat gaagcctggc atatcagccg tccgctggtg gattttggta tgcgccagcc
    14101 ggtaggtgtt ggtgatgtga cgagcggttt actgctggtg aaactgcttc agggggcaac
    14161 gctgcaggag gcgctggaac atgtgaccgc tgcagtctac gaaatcatgg tgaccaccaa
    14221 agcaatgcag gaatatgagc tgcaagtggt ggctgctcag gatcgtattg ccaaaccaga
    14281 acattacttc agcgcaacaa agctctgaaa tatctcattt cggcccacaa cgacctgtgg
    14341 gccgatggct attactttaa gccttccgct gacagtgcgt cttgtacttc cggacgttca
    14401 gccatacgtt gcataaatgc tgcaatgtgc tctaaccctt ccagattcag tttcaccgcg
    14461 tatgcccagc gcagaaccgt aaacagatag gcatcagcaa ttgtaaatct ttgcccgcag
    14521 atccagtgct catccttcag tgtctcgttc acatattgca gcttcttctc cagctgcgcg
    14581 cgaactgtcg gtttgtactc ttccggtgta tcagggcgaa acagaggtgt gaaaccttta
    14641 tgcagctcgg tggcgatgta attcagccat tcgatggttt tatagcggga aatactgttt
    14701 accggtgcca gcaactggcg gtcggggacg ctgtcggcaa gatactgcat aatcgctacg
    14761 ccttccgtca gcaaagtacc gtcatccagc agcaatgcag gcacctgccc cttagggtta
    14821 acggcaaagt aatcgtcacc gttttcgaga cgttttttca ttaaatccac actgacgagg
    14881 gtaaaatcct ttccgctctc acgcagggtg atatgggaag cgagagagca ggcacccggt
    14941 ttgtagaaca atttcatcgg taactccttt tgtctgtggt ttaaagtatg gtagtgcgtt
    15001 gctgaacaaa aaaaaagccg ctaacgaaaa gttagcggct tgtaaaagag tttccctaaa
    15061 ttttacgcta cagcagcttt cgccgctttg tctgcagcgt catcctgcgt catacggtgc
    15121 agtttaggcg cggtcagcag catcagtact gcaataatgg cagtagcgac accaatctgc
    15181 aagaatacgc gaccataaac ttccagtgac atcagcgggt cggtaacgtt atccggcaca
    15241 gccatcatac ccgcaacata accaccaatc aggtttgcac cggcagtggt caggaaccag
    15301 ctacccataa tgaagcccat cagacgctgc ggaacgagtt gtgcaaccat tgccagaccc
    15361 agaccagaga tcatcagttc cccgatgctc tgcaggccat agcttgcgac cagccagctt
    15421 acagacacga taccagcgtc agacgcgaat ttcgcaccca gcggcagaat caggaacgca
    15481 ccagaacaca tcaccatgcc gattgcaaac ttggttggca tcggcagggt atcgcccatc
    15541 ttgttataga tagcggccag aatcggacta ccgatgatga tccagaacgg gttcagtgcc
    15601 tgatactgct caggttctac ggccagaccc agaatggagt gctcaacgtt acgaatcgca
    15661 aagaagttca gtgacgttgg catctggctg tacagcacga agaagataat ggcttcgagc
    15721 atcaggatga aggcaacgat cattttacga cgcgcagcac ctttcatcgc gaaggcttct
    15781 ttaccgaaga taaccacgat accgaaggca acaacgccca gcgccatacg cgcaacttcc
    15841 tgattgtgca gcagccaggt ggcgatagcg atcagtgcca caacaccaat aatggtcagt
    15901 agcaggttac ggtagttgat aggctcgaag tctggttttg aaccgtactg tttaacccag
    15961 cgttggcaga aggcgaagtt aacgatagtg atcagcaggc ctacaacgct caacgcaaac
    16021 gcaacactcc agccgtattt cgcggccagc cacggcgtag caatcataga gaagaaagag
    16081 ccgatgttga cggacatgta gtacatggtg aatgcaccgt ccagacgcgg gtcgtttttc
    16141 tcatagcatg tagaaagcag agaagacggg ttagctttaa acaggccgtt accgaccgca
    16201 atagccgcca tacccatata aacgataccg gcgtcgtgac cagaccaggc aaccagagca
    16261 taaccaatcg ccagcacaat agcgccgagc ataattacgc gtttagtacc cagtaccttg
    16321 tcacctaacc agccgccgat agcgaccaga ccataaacca gggcactaaa ggaagagaaa
    16381 agggtgattg aatccgcttc agacataccc agttgtttaa ccaggtaaac agccataatt
    16441 ccttgtaggc cgtaataacc aaaacgttcc cataactcaa tcgagaagat gagatagaac
    16501 gccttcggtt gtttgaaagc gttcaaactg acgctttcag ttggtttttg gtttgcagtg
    16561 gacactttta cctctttttt ttacatccca tattaacggg ggtgctttct cgcgttatgt
    16621 ctaaaaaaca tacgcgcaat tgttatagtg ggaggggaaa cggcaggtaa tgttcactat
    16681 cttgcacttt cagacaagtc ctttgtaata atctgttaca tataactgag gcggggaatt
    16741 caaccattcc gggaaatgtt atccagcgtt aaatttttca aaaaacgtaa cggcagattt
    16801 tttcactgca ctgagagggg ggtgatggct taacggcgat atgttctgct atttagcgtt
    16861 tttactagtg agatagtcca gtttctgaaa aatagccagt gtaatgtttt gtaggtcaat
    16921 atttgttgcg tgtgtaaatt taaatgattt taacctgatt cttgcaatgt ttttacattg
    16981 cgacagcatc gtgatcttga tcacgaatga atagaaaatt tcgctattaa aaaaacaatt
    17041 aacctgcatt tttggttatc cgcaaacgga ttacctgatg ataacgctaa gcaatggcat
    17101 cattcacgtt aaaaagatgt tttaaaagag gataaagaaa ggttatcaat ggggtaatcg
    17161 gtgttacccc ttttcttcag atgtcaactt tctctttgta ttcacaaaga tcttcaataa
    17221 tacaagagcc acagcggggc ttgcgggcaa tgcaggtata acgcccgtgc aggatcaacc
    17281 aatggtggca gtcgacttta aactctgccg gaaccacttt cagtagcttt tcttctacct
    17341 gttcaacgtt tttccccggc gcaaattgag tacgattaca aacgcggaaa atgtgcgtgt
    17401 cgacagcaat agtcggccag ccgaatgcag tgtttaatac gacgttggct gttttacgac
    17461 ctacgccggg cagggcttca agcgcagcac gatcttccgg aacctcgcca ttatgctgct
    17521 ccagtaagat acggcaggtt ttgatgatat tttctgcttt gctgttataa agcccaatcg
    17581 ttttgatata ggttttaacc ccttcaacgc ccagttcaag catcgctgca ggcgtattcg
    17641 ccaccgggta gagtttcgcc gtcgccttat taacactgac atcggtcgcc tgagcggaaa
    17701 gcagtacggc aatcagcaat tcaaaaggcg aactgaaatt aagctcggtg gtgggatgag
    17761 gattgttctc acgcaggcga gtgaggatct ccaggcgttt tgctttgttc atcagacatt
    17821 ccctgtttca ccgtttggca atgcacgttc ggcagctgct tcagtacggc gctttttcat
    17881 tctttcatca atcaggtatt ttcctgccag catcagtccc aggccaataa atgcacctgg
    17941 tggcagcatc gccagcagga aaggggagtc ggtgtggaaa atctccacgc gtaatacttt
    18001 tgcccagcta cctaacagcg catctgcacc gtcaaacaat gtgccattgc cgataatttc
    18061 gcgtagtgaa cccagcacga acatggcgca ggttgcgccc ataccaattg aaaagccgtc
    18121 cagtgccgaa agcgccggac cttttttggc ggcgaaggct tcagcgcggc ccacaacgat
    18181 acagttagtg acaatcagcg gaataaaaat ccctaatgat tgatacaggc caaaggcgta
    18241 ggcgttgatc agcatctgta cagcgctgac caccgaggcg atgatcatca cgtaaatggg
    18301 aatgcggatc tcggctggcg tccagtgacg cagcgtcgaa atggtcaggt tggtcagcgt
    18361 cagtaccagc gtagtcgcaa gtcctaaacc cagagcatta gtggcagtgg acgtgaccgc
    18421 caacagagga caaaggccga gcaactggac cagcgcagag ttgtttttcc acaacccctg
    18481 aacaataacg tctttaattt cgctcacgct ttattctcca caggcaggaa gttgagaaag
    18541 ttgtgccggt aacgtctgag cgtacaatcc ggcgcgtttt accgcattaa ccaccgcgcg
    18601 gggagtaatc gtcgcgccgg tgaactggtc gaaatcacca ccatctttct tcaccgccca
    18661 gtgcgcatca tccgcaccac tgattttttt acccgcaaaa tgggtgatcc agtcagaaag
    18721 gcgcagttcg attttatcgc caagccctgg cgtttcgtgg tgctctgtca cgcgcgtgcc
    18781 aagtaccgtg ccgttaaaat cggctcccac cagcagctga atcgcaccgg aatagccatc
    18841 tggcgcggtt gcttccagaa cggcggctac cggtttgtca tcctgtttgg cgatgtaaac
    18901 ccgatgctca cctttaccta actctggcgc agttaccaga tagcaactct gtgccagcgc
    18961 attgttatag cgttcggctg gcagcacctg atcaaataat gccttttgtt gcagacttgc
    19021 ctgttcagca atcgtcgttt tggtcatctg gttgatggcc gcagttaacc ctgttgaacc
    19081 cgctgcaaat agcgctaacg taatgccgtg ttttcggata gttttcagca tggtttaccc
    19141 tttgcgatgg ccgtagacgc gcggacgcgt gtagtaatcg atcagaggaa ccgtgatgtt
    19201 cgccagcagg acggcaaaag ccacgccgtc aggatagccg ccgaaactgc ggatcaacca
    19261 gactaataag cccgcaagcg cgccgaaaat aagacgacca cgattggtcg tagaagcggt
    19321 aaccgggtca gtcaaaataa agaatgcgcc gagcatggtc gcaccagaca gcagatgaat
    19381 ttgcggtgct gccagtgttt ctggtgagaa caaccagccc aacgttgcgc ataacgccag
    19441 cgttactaag aagctgaggg gaatatgcca gcgaatcgct ttctgccata gcaaccacag
    19501 gccgccagcc agccaggcga gatttaccca ttgccaacca gcgcccgcca gaataccgct
    19561 gtagatcgga tattgcataa tctgttcaac cgaatgaccg gcacggacag aggttttaaa
    19621 tgtatccagc ggtgtcgcct gactaatgcc atcaatacct aagtgtagtg tgttcatatc
    19681 agcaccactg gcggtatgcc cgctgaaaat aacctgtatg gcgtcgataa aaccagggat
    19741 gttgaccgca atttcatgtg gcggtaacca gctggtcatc tgcaccggga aggagatcag
    19801 taagaccaca taaccaatca ttgccggatt aaacgggttt tgccccagac cgccatacaa
    19861 ctgtttagcg atgatcaccg caaacaccgt acccagcacg accatccacc atggcgcgag
    19921 ggggggaata cttaccgcca gcaataagcc tgtcagcaat gctgagttat ctttcaacgt
    19981 tgcggctacc gactgcttgc gtagtttgag tacgagagct tcggctaaca gagtactaac
    20041 cgatgccaac aggatctgaa cgagagtacc ccaaccaaaa aaccacagtt gtgctgcgat
    20101 tcctggcacg gctgcgagca acaccaacag cataatgcgc gatgtctggc gctggttatg
    20161 ggtataaggg gagctagcta ttctgaatac catttagtcc tcgtttacaa ccttttgctg
    20221 ggcggctttt ttggcctgag cgcgggcaat agccgcggca actgccgctt tgcgcggatc
    20281 aacctgttct tctggttctg cgttagtctg ttgctgttcc agcttgcgcg ctttggcacg
    20341 ggcaatagcg gcttcgacgg cggctttgcg cggatcaacc tgttcttcag gtaccgcatt
    20401 agcctgttgc tgttccagct tgcgtgcttt ggcgcgtgca atagccgctt cgacggcagc
    20461 tttgcgcgga tcaacctgtt cttctggctc cgcattagcc tgttgctgtt ccagtttgcg
    20521 cgctttggct cgggcaatag cggcttcgat ggcggctttg cgcggatcaa tctgttcttc
    20581 tggttccgca ttag
//
LOCUS       DADLFA010000063        20404 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000063 DADLFA010000000
VERSION     DADLFA010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20404
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.063"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            127..942
                     /locus_tag="JQH65_001924"
     CDS             127..942
                     /locus_tag="JQH65_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAY6139349.1"
                     /translation="MAQINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            1070..1651
                     /gene="sodB"
                     /locus_tag="JQH65_001925"
     CDS             1070..1651
                     /gene="sodB"
                     /locus_tag="JQH65_001925"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HAY6139350.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(1797..2972)
                     /locus_tag="JQH65_001926"
     CDS             complement(1797..2972)
                     /locus_tag="JQH65_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY6139351.1"
                     /translation="MKINYPLLALALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAA
                     GMLISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSR
                     ILTSLNHGAFFGLGSVVAASVEPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGW
                     RMSFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGA
                     MFTLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYPGGKLADRSVNGTLKGFLL
                     LLMVIMLAIPFLARNEFGAAISMVVWGAAAFAVVPPLQMRVMRVASEAPGLSSSVNIG
                     AFNLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(3138..3227)
                     /gene="ynhF"
                     /locus_tag="JQH65_001927"
     CDS             complement(3138..3227)
                     /gene="ynhF"
                     /locus_tag="JQH65_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HAY6139352.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            3526..4551
                     /gene="purR"
                     /locus_tag="JQH65_001928"
     CDS             3526..4551
                     /gene="purR"
                     /locus_tag="JQH65_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HAY6139353.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(4548..5480)
                     /locus_tag="JQH65_001929"
     CDS             complement(4548..5480)
                     /locus_tag="JQH65_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY6139354.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            5593..6804
                     /locus_tag="JQH65_001930"
     CDS             5593..6804
                     /locus_tag="JQH65_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HAY6139355.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKAGNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEAGCQNH
                     GNAEVAHSESH"
     gene            7095..8243
                     /gene="cfa"
                     /locus_tag="JQH65_001931"
     CDS             7095..8243
                     /gene="cfa"
                     /locus_tag="JQH65_001931"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HAY6139356.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSMVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(8283..8924)
                     /gene="ribE"
                     /locus_tag="JQH65_001932"
     CDS             complement(8283..8924)
                     /gene="ribE"
                     /locus_tag="JQH65_001932"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HAY6139357.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            9139..10512
                     /gene="mdtK"
                     /locus_tag="JQH65_001933"
     CDS             9139..10512
                     /gene="mdtK"
                     /locus_tag="JQH65_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HAY6139358.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSVI
                     ILQRASR"
     gene            complement(10553..11809)
                     /locus_tag="JQH65_001934"
     CDS             complement(10553..11809)
                     /locus_tag="JQH65_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6139359.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDGVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            12117..12193
                     /locus_tag="JQH65_001935"
     tRNA            12117..12193
                     /locus_tag="JQH65_001935"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12151..12153,aa:Val,seq:gac)
     gene            12198..12274
                     /locus_tag="JQH65_001936"
     tRNA            12198..12274
                     /locus_tag="JQH65_001936"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12232..12234,aa:Val,seq:gac)
     gene            12382..12687
                     /locus_tag="JQH65_001937"
     CDS             12382..12687
                     /locus_tag="JQH65_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HAY6139360.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            12813..14417
                     /locus_tag="JQH65_001938"
     CDS             12813..14417
                     /locus_tag="JQH65_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HAY6139361.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFNRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFVQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(14429..15241)
                     /locus_tag="JQH65_001939"
     CDS             complement(14429..15241)
                     /locus_tag="JQH65_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6139362.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFRRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIAIAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGSGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(15245..16030)
                     /locus_tag="JQH65_001940"
     CDS             complement(15245..16030)
                     /locus_tag="JQH65_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HAY6139363.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(16027..16695)
                     /locus_tag="JQH65_001941"
     CDS             complement(16027..16695)
                     /locus_tag="JQH65_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAY6139364.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(16759..17397)
                     /locus_tag="JQH65_001942"
     CDS             complement(16759..17397)
                     /locus_tag="JQH65_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HAY6139365.1"
                     /translation="MNHRDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAELYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(17410..19511)
                     /locus_tag="JQH65_001943"
                     /pseudo
     CDS             complement(17410..19511)
                     /locus_tag="JQH65_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246021.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            complement(19481..20158)
                     /locus_tag="JQH65_001944"
     CDS             complement(19481..20158)
                     /locus_tag="JQH65_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HAY6139366.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRGIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITVRILQRKKSWL
                     TVGQVIY"
BASE COUNT         5108 a         5312 c         4765 g         5219 t
ORIGIN      
        1 tcgccaacga aacgtatttt ttaacaataa aagctattaa ctttctcttc ttctatgcat
       61 tagaatcatc aagttttgta aatcagacgc aggcatgata gacctgcctt aacagaggga
      121 cgctcagtgg cgcagataaa ccgtatttcg atcacgctct gtgctttact ttttaccacc
      181 ctgcctttaa cgcctatggc ccatgcttca aagcaagcca gggagagttc tgctaccact
      241 catatcacca aaaaagcaga taaaaagaaa agcacggcaa ccaccaaaaa gacccagaaa
      301 acagcgaaaa aagccgccag taaaagtacg accaaaagca aaaccgcttc ttccgttaaa
      361 aaatcttcca ttaccgcttc taaaaacgcc aaaactcgca gcaaacacgc cgtcaataaa
      421 acggcctcag ccagctttac cgaaaagtgt accaaacgta agggctataa atcgcattgt
      481 gtgaaagtca aaaatgccgc gtcaggaact cttgccgacg cgcacaaagc gaaggtgcaa
      541 aaagctacga aagtggcaat gaataaactg atgcagcaaa ttggtaagcc atatcgttgg
      601 ggtggcagct caccgcgtac cggttttgat tgcagcggcc tggtttatta cgcttataaa
      661 gatttggtga aaattcgtat tccgcgtacg gcgaatgaaa tgtatcacct gcgtgatgca
      721 gcgccaatcg aacgtagtga actgaaaaac ggcgacctgg tctttttccg tactcaggga
      781 cgcggcacag ccgatcatgt cggcgtgtat gtcggcaacg gcaaatttat tcagtcaccg
      841 cgcacaggtc aggaaattca aatcacttct ctcagtgaag actactggca gcgccactat
      901 gttggcgctc gtcgggtaat gaccccaaaa acacttcgct aaaactttac cctgttgtta
      961 cggcaacagg gtaagttcat cttttgtctc accttttaat ttgctaccct atccatacgc
     1021 acaataaggc tattgtacgt atgcaaatta ataataaagg agagtagcaa tgtcattcga
     1081 attacctgca ctaccatatg ctaaagatgc tctggcaccg cacatttctg cggaaactat
     1141 cgagtatcac tacggcaagc atcatcagac ttatgtcact aacctgaaca acctgattaa
     1201 aggtaccgcg tttgaaggta aatcactgga agagattatt cgcagctctg aaggtggcgt
     1261 attcaacaac gcagctcagg tctggaacca tactttctac tggaactgcc tggcaccgaa
     1321 cgccggtggc gaaccgactg gaaaagtcgc tgaagctatc gccgcatctt ttggcagctt
     1381 tgccgatttc aaagcgcagt ttactgatgc agcgatcaaa aactttggtt ctggctggac
     1441 ctggctggtg aaaaacagcg atggcaaact ggctatcgtt tcaacctcta acgcgggtac
     1501 tccgctgacc accgatgcga ctccgctgct gaccgttgat gtctgggaac acgcttatta
     1561 catcgactat cgcaatgcac gtcctggcta tctggagcac ttctgggcgc tggtgaactg
     1621 ggaattcgta gcgaaaaatc tcgctgcata ataactgatg gcaaatgcag cattgcctga
     1681 tgcgcttcgt ttaccatgcc aacgcggatg aggcgttaac agcgcattcg gcaagataaa
     1741 tcgcacgttg tcagcaactg taacgcagaa ggttatcctt ctgcgttttt gtttaattag
     1801 ctgttagcaa cgcaaactgt ttcaggttgt tttctggctg acataaacac cagcaataat
     1861 gccagtcccg cgacaatcgc tcccatcacc ggcacaaagc tgtatcccag cccagcggaa
     1921 attaccgcac caccagcagc tgctcccagc gcatttccaa gattaaaggc accaatattg
     1981 actgatgaag acagacctgg cgcttcactg gcgacacgca tcacgcgcat ctgtaacggc
     2041 ggtacgaccg caaaggctgc tgcgccccac accaccatgc taatagctgc gccgaactca
     2101 ttgcgggcca ggaacgggat tgccagcata atcaccatca acaacaacaa aaagcctttc
     2161 aacgtgccgt taactgaacg atctgccagt ttgccgccgg gatagttacc gatagagaat
     2221 ccgacaccaa tcagcaccag cattgccgtg acgaacaccg gtgttgcgtg ggtaatactt
     2281 tgcagtaccg gagagatata ggtgtagaga gtaaacattg caccagctcc cagtaccgtc
     2341 gtcagcaatg cagacaacac ctgcggacgc attaataccg ccagctcttt tttcacttca
     2401 ggtcgtgccc ctgcaccacc tttaggtaat gagaagaaca gacttaccat tgaaatcact
     2461 cccagccccg ccgttgccag aaatgacatc cgccagccga tggtttcacc caaccaggtc
     2521 gccgccggca cgccaccgat atttgccagg gttaacccca taaacatagt ggcaactgcg
     2581 ctggcctgtt tatgttttgg ctccacgctt gcggccacga ctgaacccaa accaaaaaat
     2641 gctccgtgat tcaggctggt caaaatgcgt gaaagcatca gggtcatata atccggcgcg
     2701 atggcggaaa gtacgttgcc gagcgtgaaa attgccatca ggaaaatcaa cgcactgcgg
     2761 cgggcacgat gagaaagtag aagcgtcatc agcggcgcgc caaccattac gccaactgca
     2821 taggcactga ttaacattcc ggcagcggga atcgagacat ccacaccgcg cgcaatgacg
     2881 ggcaacaagc ccattggcga gaactccgtt gtcccgatac caaacgcgcc aatcgccagc
     2941 gccagcgcca gcaacggata gttaattttc atgccttatc tccacctctt cgcgtcatta
     3001 cgcgatattc attaaagtgg cgaaagcatg acagcaatca caaaaaaatg aaaataacaa
     3061 aaagagaaaa cacttttgcc attttgctaa caaacaggaa ggagatgcga gggagaacgc
     3121 gctccctcga gaggaaatca gtgcagcgcg gcagtcaaac ccacggccac gatcaaaccg
     3181 aggacgataa tcgttgttac cagtgaaaat ttaaggtcgg tgctcatcaa gttttctcct
     3241 tttttattac cacacaaata gtgatattac gcatttttac acactgtgat gaaaaaatct
     3301 cccgtcattt ataatgataa gtgtttttac cacttcccct tttcgtcaag atcggccaaa
     3361 attccacgct tacactattt gcgtactggc cattgacccc ttcctgacgc tccgtgtcgt
     3421 ttttccggcg taccgcaaca cttttgttgt gcgtaaggtg tgtaaaggca aacgtttacc
     3481 ttgcgatttt gcaggagctg aagttagggt ctggagtgaa atggaatggc aacaataaaa
     3541 gatgtagcga aacgagcaaa cgtttccact acaactgtgt cacacgtaat caacaaaaca
     3601 cgtttcgtcg ctgaagaaac gcgcaacgcc gtgtgggcag cgattaaaga attacactac
     3661 tcccctagcg cggtggcgcg tagcctgaag gttaaccaca ccaagtctat cggtttgctg
     3721 gcgaccagca gcgaagcggc ctattttgcc gagatcattg aagcggttga aaaaaattgc
     3781 ttccagaaag gttacaccct gattctgggt aatgcgtgga acaatcttga gaaacagcgg
     3841 gcttatctgt cgatgatggc gcaaaaacgc gtcgatggtc tgctggtgat gtgttctgag
     3901 tacccagagc cgttgctggc gatgctggaa gagtatcgcc atatcccaat ggtggtgatg
     3961 gactggggtg aagcaaaagc tgacttcacc gatgcggtca ttgataacgc gttcgaaggc
     4021 ggctacatgg ccgggcgtta tctgattgaa cgcggtcacc gcgaaatcgg cgtcatcccc
     4081 ggcccgctgg aacgtaacac cggcgcaggc cgccttgccg gttttatgaa ggcgatggaa
     4141 gaagcgatga tcaaggtgcc ggaaagctgg attgttcagg gtgactttga acccgaatct
     4201 ggttatcgcg ccatgcagca aatactgtcg cagccgcatc gccctactgc cgtcttctgt
     4261 ggtggcgata tcatggcaat gggcgcactt tgtgctgctg atgaaatggg tctgcgcgtc
     4321 ccgcaggatg tttcgctgat cggttatgat aacgtgcgca acgcccgcta ttttacgccg
     4381 gcgctgacca cgatccacca gccaaaagat tcgctgggtg aaacagcgtt caacatgctg
     4441 ttggatcgta tcgtcaacaa acgtgaagaa ccgcagtcca ttgaagtgca tccgcgcttg
     4501 attgaacgcc gctccgtggc tgacggcccg ttccgcgact atcgtcgtta atcacccgtt
     4561 gcgggagtct cttccggctc ccgcagccac tccttattca gcgtctcact atcgccgaga
     4621 tactcaagca gccaggttaa cgcaggcgac atatcatttt gctgccatgt cagacaacat
     4681 gccgaatccg ggaaggggtt ttccaattca agtgctaccc acttcccctc attaagccac
     4741 ggtttggcga aatgtgttgg caccatccct atgcataatc ctgccgagat acaggttgcc
     4801 gatgattccc agtcaggcac gacaactctt ttttggttat ccagtaacca ggtaatacgt
     4861 ttaggtagcg ttcgcgaggt gtcttcgcgc accaacgacg gccagttgcg caacgtatca
     4921 tcgctgaatg ggccatccat caacgccagc gggtggtggc tggcaacaac gcaactccag
     4981 cttagcatcc ccatatcccg gaaggcataa cgaccgccta ccggaatcgc gcgtgttgcg
     5041 ccaatcgcca gttccacgcg cccgtcggaa agcgcatccc agacaccgtt gaacacttcc
     5101 tgaaagacaa gaagttcgac atcatcaaaa tggcgataaa aatcaacgat catctgccgt
     5161 gtacgttctg gcctgacaat attatccact gcgatagcta actgaccgcg ccagccgttc
     5221 gctatctgct gacattgctg gcgggtgatc tgcatttttt tgacaacaga gcgcccttct
     5281 ttgagaaacc acgctccagc agcggtcagc tccacatcac ggtgccgtcg ttcaaagagc
     5341 ggcaccgcca gccactcttc cagctgacgc acggtatagc tgaccgcaga aggaacgcga
     5401 tgcagctcct gtgccgcagc gctaaaacta ccattacgcg ctaccgcatc aacaacttcg
     5461 agtgaatatt ctgaccacat agtctgcctg caaaattttt gaaatcagtc atcaaatatt
     5521 accgtttcac aacactaatt tcactcccta cactttgcgg cggtgtttaa ttgagagatt
     5581 tagagaatat acatgcaacc tgggaaaaga tttttagtct ggctggcggg tttgagcgta
     5641 ctcggttttc tggcaaccga tatgtatctg cctgctttcg ccgccataca ggccgacctg
     5701 caaacgcctg cgtctgctgt cagtgccagc cttagtctgt tccttgccgg ttttgccgca
     5761 gcccagcttc tgtgggggcc actctccgac cgttatggtc gtaaaccggt gttattaatc
     5821 ggcctgacaa tttttgcgtt aggtagtctg gggatgctgt gggtagaaaa cgccgctacg
     5881 ctgctggtat tgcgttttgt acaggctgtg ggtgtctgcg ccgcggcggt tatctggcaa
     5941 gcgttagtga cggattatta tccttcacag aaagttaacc gaatttttgc gaccatcatg
     6001 ccgctggtgg gtctatctcc ggccctggct cctctgttag gaagctggct gctggtccat
     6061 ttttcctggc aggcgatttt cgccaccctg tttgccatta ccgtggtgct gattctgcct
     6121 attttctggc tcaaacccac gacgaaggcc ggtaacaata gtcaggatgg tctgaccttt
     6181 accgacctgc tacgttctaa aacctatcgc ggcaacgtgc tgatatacgc ggcctgttca
     6241 gccagttttt ttgcatggct gaccggttca ccgttcatcc ttagtgaaat gggctacagc
     6301 ccggcagtta ttggtttaag ttatgtcccg caaactatcg cgtttctgat tggtggttat
     6361 ggctgtcgcg ccgcgctgca gaaatggcaa ggcaagcagt tattaccgtg gttgctggtg
     6421 ctgtttgctg tcagcgtcat tgcgacctgg gctgcgggct tcattagcca tgtgtcgctg
     6481 gtcgaaatcc tgatcccatt ctgtgtgatg gcgattgcca atggcgcgat ctaccctatt
     6541 gttgtcgccc aggcgctgcg tcccttccca cacgcaactg gtcgcgccgc agcgttgcag
     6601 aacactctac aactgggtct gtgcttcctc gcaagtctgg tagtttcctg gcttattagt
     6661 atcagcacgc cattgctcac caccaccagc gtgatgttat caacagtagt gctggtcgcg
     6721 ctgggttaca tgatgcaacg ttgtgaagaa gctggctgcc agaatcatgg caatgccgaa
     6781 gtcgctcata gcgaatcaca ctgatctata tcgatatact tatacttagg ctgctaacaa
     6841 aattttgttg tatgattgaa attagcggcc tatactaatt tcgagttgtt aaagctacga
     6901 taaatattat gtttttacgg ggacaggatc gttcccgact cactatggat ggtcatttcg
     6961 gcaagggttc ctcctttccc tctgttctac gtcggattat agactcgcgg ttttttctgc
     7021 gagatttctc acaaagccca aaaagcgtct acgctgtttt aaggttctga tcaccgacca
     7081 gtgatggaga aactatgagt tcatcgtgta tagaagaagt cagcgtaccg gatgacaact
     7141 ggtaccgtat cgccaacgaa ttacttagcc gtgccggtat agccattaac ggttctgccc
     7201 cggcggatat tcgtgtgaaa aaccccgatt tttttaaacg cgttctgcaa gaaggctctt
     7261 tggggttagg cgaaagttat atggatggct ggtgggaatg tgaccgactg gatatgtttt
     7321 ttagcaaagt cttacgcgca ggtctcgaga accaactccc ccatcatttc aaagacacgc
     7381 tgcgtattgc cggcgctcgt ctcttcaatc tgcagagtaa aaaacgtgcc tggatagtcg
     7441 gcaaagagca ttacgatttg ggtaatgact tgttcagccg catgcttgat cccttcatgc
     7501 aatattcctg cgcttactgg aaagatgccg ataatctgga atctgcccag caggcgaagc
     7561 tcaaaatgat ttgtgaaaaa ttgcagttaa aaccagggat gcgcgtactg gatattggct
     7621 gcggctgggg cggactggca cactacatgg catctaatta tgacgtaagc atggtgggcg
     7681 tcaccatttc tgccgaacag caaaaaatgg ctcaggaacg ctgtgaaggc ctggatgtca
     7741 ccattttgct gcaagattat cgtgacctga acgaccagtt tgatcgtatt gtttctgtgg
     7801 ggatgttcga gcacgtcgga ccgaaaaatt acgataccta ttttgcggtg gtggatcgta
     7861 atttgaaacc ggaaggcata ttcctgctcc atactatcgg ttcgaaaaaa accgatctga
     7921 atgttgatcc ctggattaat aaatatattt ttccgaacgg ttgcctgccc tctgtacgcc
     7981 agattgctca gtccagcgaa ccccactttg tgatggaaga ctggcataac ttcggtgctg
     8041 attacgatac tacgttgatg gcgtggtatg aacgattcct cgccgcatgg ccagaaattg
     8101 cggataacta cagtgaacgc tttaaacgga tgtttaccta ttatctgaat gcctgcgcag
     8161 gtgctttccg cgcccgtgat attcagctct ggcaggtcgt gttctcacgc ggtgttgaaa
     8221 acggccttcg agtcgctcgc taaaggctat tctatcgccc cctctccggg ggcgatttca
     8281 gatcaggctt ctgtacctgg ttgattcatg gcattttctc gtgccgccag cacacgttct
     8341 accgtatcta ccactgcctg agtttgtgga tcaatttcaa tgttgacgcg tgcgccgagt
     8401 tttttcttcc caagagtcgt gcgttccaga gtttccggaa ttaaatggac gcaaaaacgc
     8461 gttggcgtga cttcgccgac ggtcaggcta ataccgtcga tgccaataaa tcctttgtac
     8521 aggatatatt tcatcaactg actatcctgg actttaaacc agatctggcg attattttcc
     8581 gaggttaata ttttcgccac ttcagcggtg gtcataatat gacctgacat taagtgtccg
     8641 ccaatttcat cactgaattt cgccgcacgc tcaacgttta cccaatcccc cacttttaaa
     8701 tcgccaagat tggtaatgcg taacgtttct ttcatcaggt caaaactgac atggttgccg
     8761 ttaatttccg tcacggtcag gcagcaaccg ttatgcgcca cggaagcacc ggtttccagg
     8821 ccgtccagca tgtggtcggg taactccacc acatgcgtac gaaaatttgg tttctcgtca
     8881 atcgacacca gttttgcggt gccctgtaca atccccgtaa acatacttac aactcctgaa
     8941 atcagttaag acattctgtt cagcacaata gcaggtggca aacgccctta ccagtgaagg
     9001 ggtaagaatg actatttttt cactggagaa ttaataaatc ctcgctacaa tagactgaat
     9061 ttcccctgct tcttcttttt gctgcccatt caggcggctt tttagtctct catataacta
     9121 caaataaaag gtgttcacgt gcagaagtat atcagtgaag cgcgtctgtt attagcatta
     9181 gcaatcccgg tgattctcgc gcaaatcgcc caaactgcga tgggttttgt cgataccgtg
     9241 atggcgggcg gctatagtgc caccgacatg gcggcggtcg ctatcggtac ttctatctgg
     9301 cttccggcga tcctctttgg tcacggactg ctgctggcat taacgccggt tatcgcgcaa
     9361 ttaaatggtt ccggtcgacg tgagcgcatt gcgcatcagg tgcgacaagg tttctggctg
     9421 gcaggttttg tttccgttct cattatgctg gtgctgtgga atgcaggtta cattatccgc
     9481 tccatggaaa acatcgatcc ggctctggcg gacaaagccg tgggttatct gcgtgcgttg
     9541 ttgtggggcg cgccgggcta tctgttcttc caggttgccc gtaaccaatg tgaaggtctg
     9601 gcaaaaacca agccgggtat ggtaatgggc tttatcggcc tgctggtgaa cattccggtg
     9661 aactatatct ttatttatgg tcatttcggt atgcctgagc tcggtggcgt tggttgtggc
     9721 gtggctactg cggcggtgta ttgggtcatg ttccttgcca tggtttctta cattaaacgc
     9781 gcccgctcca tgcgcgatat tcgtaacgaa aaaggcaccg cgaaacccga tcctgcggtt
     9841 atgaaacgac tgattcaact cggtttaccg attgcgctgg cactattctt tgaagtgaca
     9901 ctgtttgccg tcgtggctct gttagtgtct ccgctcggta ttgttgatgt cgcaggacac
     9961 cagattgccc tgaactttag ttcactgatg tttgtgcttc caatgtcgct ggcggcagcg
    10021 gtaactatcc gcgtaggtta tcgtctgggt cagggctcaa cgctggatgc gcaaaccgct
    10081 gcgcggaccg ggcttatggt gggtgtctgt atggcaaccc tgacggccat tttcacggtt
    10141 tcactccggg agcaaatcgc cctgttgtac aacgacaatc ccgaggttgt aacgctggct
    10201 gcgcatttga tgttgctggc ggcggtatat cagatttctg actcaatcca ggtgattggc
    10261 agtgggattt tgcgtggtta taaagatacg cgttccattt tctatatcac ctttacagct
    10321 tactgggtgc tgggcttgcc aagcggctat attctggcac tgaccgatct ggtcgttgaa
    10381 cctatggggc cagcaggctt ctggataggc tttattatcg gcctgacatc ggcagccatt
    10441 atgatgatgt tgcgtatgcg gttcctgcaa cgtctgccgt cagttatcat tctgcaacga
    10501 gcatcccgct aataaagaca aggcgcaacc ttcacgggtt gcgcctgtgt ttttacgcag
    10561 gctggagcgt tgcgccaatc ccgtcttcgt ctggctgtaa tttcagagcg ttgcacagag
    10621 tttgccgact ggcatctgtt atctcaacgt gtatatcacc ggtaacttcc cctttcacat
    10681 tgatgaagtc aggtgagatt tctttcgcac tggtttgcag gcaaatcgct ccctctccgc
    10741 gcaggcgttt cactttcagt tgcacatgcg ttgccgcacc agtgatcagc cacagttttc
    10801 cgccattcag attgatgtta ccaacaataa aagtgacacc gtcacctttt accgtactgc
    10861 cgtcgagaat caacacaccg ccctgctgta cagtaatcgt tgattgttgc agatctgcgc
    10921 catagccaat aatcattgaa gcgccgtcca gtaatgcaac actcccggtc agttcaaccg
    10981 gtgcgcgatc ttcctctacg acaaaatttt cgtccgcgct ggtgggtgac aacaggatca
    11041 ctgttccacc ttgtattacc gcattttcca gcgtaccggc atgcacttca gcccgaccac
    11101 ccacattcac ggacaggttt tgcgtcttac cggaactgta gagctgaagg ccatccgtcc
    11161 ccagacgata aatggatcca ttctcaacaa tggtgtcaga gtcttcacca ccgagattat
    11221 tcatggtgcc accagcaagc aacgtatcac tggctttccc ggccagcata aaaacgccgc
    11281 cctgattaat ctcacaattg ctggcttccc cacctggata caccaacaat tcaccacctt
    11341 catctaccac gaccgctgag gttgtcccat taaccaacag gccgccctct tgatcgagaa
    11401 tgattttttc cgcgcgatgt ccttccagta cacgcaggtt accgccattt tccagcaaca
    11461 aaccgcaggc ataaccctga tcaacgctga attcaccttc gggatggcgg ccattaaccg
    11521 tagcgagcgt agagagacta attgcgcctc cctcggactg aacaatatct gtggcaatgg
    11581 caccgtcgct gaccgatagc gtgccattgt taccgtactg tgtgcatttg gcgatcccac
    11641 cggttgccac tttcaaccag ccagcccccg tgactacggt attttcggct cttccgccag
    11701 cttcaacaat caactcgcct tcagtaagtt gcgtcgcgcc aatgacctcg ccggtcttaa
    11761 caatttgcac attcccgtcg ctcatcaagt ttttcgcatc agatcccatg atttattcct
    11821 ttgctgcatc tgtgtgcctt tattgctacc taaatgtaaa ggctacggag gatttatcca
    11881 cgacagattt gagatggtgg caaacaactc tgtttaaact ctgatacacg aattattggg
    11941 ttgtatcaga tgtaaatgcg atcctgaata aaaatcgccc ttgcaaatca acaaaatata
    12001 gccaatttgc ttaaaagctc tgcaaacggt cgaaatagcg cagaaaatta cgttttgcct
    12061 cttgccacct tcccactctg ccgctaatat tcgtccccgt tgtcacctac aacgttgcgt
    12121 tcatagctca gttggttaga gcaccacctt gacatggtgg gggtcgttgg ttcgagtcca
    12181 attgaacgca ccatcctgcg tccgtagctc agttggttag agcaccacct tgacatggtg
    12241 ggggtcggtg gttcgagtcc actcggacgc accagatttt cttaatctgg tcttctcctt
    12301 tttccctctg tttcttctct gtatccaata cgttaaaaga tttacactgt cttcgtatgc
    12361 gttatcagaa ggagaatcgc tatggcaact ttgttacaac ttcattttgc ttttaatggc
    12421 ccatttggcg acgcaatggc tgagcagctt aaaccgcttg ctgagtcgat taatcaggaa
    12481 cctggttttc tgtggaaggt atggacagaa agtgaaaaga accacgaagc cggtgggatc
    12541 tatcttttta ctgatgaaaa aagcgccctt gcctatctgg aaaaacatac tgcccgactt
    12601 aaaaatctcg gcgttgagga agttgtcgcc aaagttttcg atgtcaatga gccactgagt
    12661 caaatcaatc aggcaaaact cgcctgacag aatttaatca agggcggtta gcgccctttt
    12721 catccctgtc tgaaatttct caaattctaa aaatctcaac caaacttatc tgataacact
    12781 aaattcgaaa gaatgcgtac aggtaagtaa caatgaaaaa aattgctatt gtgggtgccg
    12841 ggcctacggg gatctacacc ttattctcgc ttctacagca acaaactcca ctttctattt
    12901 ctatcttcga gcaggctgac gaggccggtg tcgggatgcc atacagtgat gaggaaaact
    12961 caaaaatgat gctggcaaat attgccagta ttgaaatacc gccgattaat tgtacgtatc
    13021 tcgaatggct acaaaagcaa gaagccagcc atctccagcg ttatggcgtt aaaaaagaaa
    13081 ccttgcacga tcgtcagttt ttaccgcgaa ttctgctggg cgaatatttc cgcgatcaat
    13141 ttttacgatt agtagaccag gcacgacagc aaaaatttgc agtggctgtt tatgaatcat
    13201 gccaggttac cgatctgcaa attacaaatg ctggcgtcat gctcgctaca aatcaggatt
    13261 tacccagcga gacgtttgat ttagcggtga ttgccacggg tcacgtctgg cctgatgaag
    13321 aagaagcaac cagaacgtat tttcccagtc cgtggtcagg cctgatggaa gcaaaggtcg
    13381 atgcgtgtaa cgtgggtatt atgggaacat ccttgagcgg actggatgcg gcaatggcag
    13441 tggctattca gcatggttcg ttcattgaag atgataaaca acacgtcgtt tttaaccgcg
    13501 ataacgcaag tgaaaagcta aatattacgt tgatgtcgcg cacgggtatt ttacccgaag
    13561 ccgatttcta ttgccctatt ccctacgagc ccttacacat cgtcaccgat caggcattaa
    13621 atgctgagat tcaaaaaggt gaagagggcc ttttggatcg ggtatttaga ttgatagtag
    13681 aggaaatcaa gtttgctgat ccagactgga gccaacgcat agccttagag agcctgaatg
    13741 tcgattcctt tgttcaagcc tggtttgccg agcgcaaaca acgcgaccca tttgactggg
    13801 cagaaaaaaa tctccaggaa gtcgaacgca ataaacgaga aaaacatact gttccctggc
    13861 gttatgtcat tctgcgcctg catgaagccg tacaggaaat tgtaccacat ctgaatgaac
    13921 acgaccataa acggttcagt aaaggccttg cccgggtttt tatcgataat tatgcggcaa
    13981 tcccttcaga gtctattcgt cggctgctgg ccttacgtga agcggggatc attcatattc
    14041 tcgccctcgg tgaagactac gaaatggaaa ttaatgagtc gcgcaccgtc ctgaaaacgg
    14101 aagacaacag ctactcgttt gacgttttta ttgatgcccg cggacagcgt ccgcttaaag
    14161 tgaaagatat tcctttccct gggctacgcg agcaattaca gaaaacaggg gatgaaatcc
    14221 ctgatgttgg cgaagattat acgttacagc aacccgaaga tattcgtgga cgcgtagcgt
    14281 tcggcgcgtt gccctggttg atgcacgacc agcctttcgt tcagggactt acggcatgtg
    14341 cagaaattgg tgaggcgatg gctcgggcgg tcgtaaaacc tgcatcccgt gcacgtcggc
    14401 gtctttcgtt tgattaaagg taaagctatc agcactcaac aaactgatgc tgttcattaa
    14461 gacgatagcg gactccggtt cgaatattgt tttcaccttc aatggcgacg gcgaagcgaa
    14521 ctctttcgcc gtcatgatat gccagtgcgg cgcttccccc tgacccgaga ataattgagt
    14581 cgaccacgcc cgtgctcact accgttgaat tttcaccgct ggcaatgatg tgtacattgt
    14641 cgccgctgtt ggcaattctg gcgtttgcgc caaagctggc gatttgcacc aggtcaccat
    14701 tactggcgac atgacaccgt tcacccaatg tacaaacgcg tactcgcatt ccggtattcg
    14761 caatccgact actatctcca gcgctgctta tccgcgaaga attaccggtt acagcaaccc
    14821 gtgcgcgttc accggagctg ccaatgtgac tgttaaagcc gacacttcca atccgcacgc
    14881 tataacccat actggcaatt tgcgcggcat acccagcgat tgcgattcgg gcgttatcct
    14941 cacaacagcc gatgcgagca tgatcgtcgc tgcgggcaaa ctgactgcaa aatggtatct
    15001 ctccggggtc tgtggccagt ttttgcatag aggaaacttc ctgatgcgcg aagttttcat
    15061 ccgccagcca ttttttccac gcatactcaa ccagactctc cgcccagtcc gtatatcctt
    15121 cctcaatcag tgcatgatga atatcggcat aactaccgcc gcgtcggaaa tggcgcagga
    15181 accagcgata catcaccgct ccgatacgcc attctcgcag gtctgctcgt gtaatgatca
    15241 ttcgttagtg ccggtgatag ccatcgacca tgcttttaaa ggtttcgtgt ggcgtacgcc
    15301 ccgtggtgca aagataaaga tgtccgaaga taaaaaagag gcttataaat gccagagcaa
    15361 aatgcgcctg caataaccag tatcttacgc caggaaacac atctcccacg gcttgcggat
    15421 agagacacaa cagccccgtc aatagtaaca acggcagcaa tccatacatg acaccaacat
    15481 aggcgacctg ctgtaaggga ttaaatttag actgtgttgt tgccgggaaa ggatgttctt
    15541 ccccctgcat aatgccaaac aaataaaatc gcgtttgttt tgccgctcgt tccagccacc
    15601 cctgacgacg aatgcgatag tggtgaccat tatccccaac ggcattgatc agcacaaagc
    15661 cgagccagca tgccagtaac aaaaatccgc aaacttcatg caccgcaacc agacttttaa
    15721 ccgcagttgc gcccaccatc gcaaagtgat ttatcagccc acttgccagc aacaatacaa
    15781 agagtaacgc attcgaccag tgccataatc tgaccgcttt gctgtaaaga taaaccttct
    15841 caccatgatc ggtggctgat ttcttcacgc cacgagcacg aagcaaggcg tgcagcccca
    15901 gcaccaacca catcccaacc agcaatactc cggcaatgat caaccacacc ggccaaaatt
    15961 cgggagtgaa ctgtggcaca tagttcgcta actggctctg aaactgttca gcatgttgcg
    16021 acgggttcat acattttcct ttttaatcaa atgttgaccg aaccggcgat acagatgcgg
    16081 tttcccggca ccaggtagct gatattgata gtatttatta tcctgcaacc acgcctgaat
    16141 ttctgggcta tcttcgcgac caaagatcaa tgcgtgttcc ggacaggcgc tgacgcaaat
    16201 gggcggaaag ccttttgcca gtcgggactc agcgcagaaa tcacatttat ctgccacttt
    16261 agtgacaggg ttaaggtagc gaacctggta cggacaagcg ccaatgcaat aactgcagcc
    16321 gatgcactgc gatttttcta cccgcacgat cccctgctca tcacgccacg atgcccccgt
    16381 cgggcaaacg tcgatgcacg gagcatcttc gcagtgctga catgactggc gaaaaaagtg
    16441 atactgcgtt tcgttgtcgt tatcggtgac tggaatgtgt gctatcgata aacggcttcc
    16501 ctgagcgggg acgtggttag ttttacggca tgcgcgggcg caaatattac agccattgca
    16561 ccgtgactcg tcatgaatca tggcataccg aacttccttt tcttgcctcg tgttcgctaa
    16621 cagagaagac gcagaaccag taaaaaagat tactgttccc atccccagaa caaatttgcg
    16681 tcgagtgaag gacatctgat tatcccctaa tgcagtggct gcaactgtcc accctatcca
    16741 tgacatctcc ctgaaatttc aggggttctg aaactgctct acctcgaacc attcgcataa
    16801 gccataagcc ttgccatagg tcataaatcg ctcactaaac aagtacaact ccgccgtcga
    16861 tgactccacc tgccgtatat cggcatattc aggtgcaaca tccatcgcgg cgatcgcggc
    16921 ttcaatctgc gcttcctgga agtaatatgg cgcttgcatc agcatggcga ctttgtaagg
    16981 acgaggatat gtctggcact caaaacgaac ggcgtgtgca atggcgcgac aaatgtcctg
    17041 ctccacaact tgcaaagaca tcgccgcgta gttttcactc atggcctgcg ttgagtaata
    17101 gtaatcatcc tggcttcccg tcatacaagc gatatcagcg tagtctccgt tctgcttaat
    17161 gctttccagc aaaactgaca gctcctcttc tgcaaccgaa tagggtggcg agagaaacac
    17221 ggcgcgcgcc accagctgcc cagccgcaga atgctggcga atgaactctg ccaggatttc
    17281 agccggttct ggttcggtta ccgtatcaac ggggtgacgc atcccctgta accattgccg
    17341 cttcgcctcg tcaacttcgc tgactttcgc taacggcaat tcatcccgat ggttcatctc
    17401 acccattcct tacacaggca gtagattgtg tgctgccaga tcggcggcaa tatcttccag
    17461 ccccagacgc tgcaatgttt cgcgggtggg actaccaagc tgtgggtccc agcccatttc
    17521 tttgtagaac atcgtgagcg aagcatgcat atcgtcacga tccattttgt cagtaccttc
    17581 agtaaagacc gggatctgcg gatccttgtc gaatacccag gaacagataa gatcgtgttc
    17641 attacgcata tctttggttt gcataagttt taccgtgtag gcacgatgca acgtaaagat
    17701 acgctctgca gctaaatcta atttttcctg agtcatctct tcgccggtga tcgctttgaa
    17761 gaatttggct tcaagcgcca gatcgccccg gtaattacgg cttttcagcg gcgaaacggt
    17821 cattggccag acccagttac acagggtgac ggcgttatgc aaacagaccc gcaacagcga
    17881 ccacttagca tatttaattt ttgcgtcatt gattggcgtg tagtttttgg tttcatcgta
    17941 ggcatcttca gaaccaaaaa gctctttcgc cacttcacgt tgcagtttca atggcaagcc
    18001 ggaaccaata aagttgatat gggtatgtgt catgcagtca cggttgaaca tacagttaac
    18061 aatggaaccg acctgcgctg acgcttcatt ggcatggtga accggatagc caaacggcga
    18121 ccagagttta tttttcgcgt agccccagta ctcttcaccc aaattccagc gttctgcgat
    18181 ggcatatgaa ccatcagcca ggtgactcag ctcacccaca cgatgcgcca gacggtagta
    18241 aaaatcttta atgaaattaa cgtcacccgc ttccagttga tcccagtgaa tttccgcata
    18301 ctcttcagca ggcagaacac acttgaacac ccctttgctg taacaataag taaagtcgcg
    18361 atgcaactgc ccgtagttac accataggcc gtagtcatcg aacaaattca gaccaaccaa
    18421 gttaccaatc acacggccat cgtctttatc ttcaaaatct ttcggaccgt tcgggaagat
    18481 ggtggtatgg acaaagttgg ctacacaagt gttaccacct gtgctgggca cgccaaactc
    18541 tttcacccga ggaatattca tttgggtcat acaacggatc gggcaagagt ggcagccact
    18601 catttttact gtgtatttct ctgccgccgg acctaagtca aaaacggatt tataggtacg
    18661 aaagccgacc gtgttctgat tgcctggcgg aatttccccc gtttcaatcg gaccaccttc
    18721 agccgcgccc caaaacagcc ctttacgtgc tgtccaacgt gacttgggat ctgaatactc
    18781 tgcccacgat tgcggcgtac ttggcacgac atggttgtta ttcgcaccaa taagttcagt
    18841 catcatgtaa tcgttgagac gcttcatctc ctgacgatcg gcaatgttga cccctttcgt
    18901 cccttcaacc gcaatcgctt tcaggttttt cgaacccata attgcgccag ttcccgcacc
    18961 gccgctgtgg ttacggctat tcaacatgcc agagagagga acaaggtttt ccccagcctg
    19021 gccaatagcc gccacacagg tttccggact ggtcaatcga caaatttctt ccgtcgttgc
    19081 gcgcgtccct tttccccata agaaatcggc tttttccagg ctaactttgt catctttaat
    19141 cttcagccat accggtgatt tcgccttccc ttcgataata atgacgtcgt atccagcgaa
    19201 tttcatttgc gctgcaaaaa gccacccata tgggcatcga caactaaatt tcctttggta
    19261 aaagtagaaa gtgaggtgat atttacgcga gaactacagg gggcaccaga tccagttaat
    19321 gggccggtag caaagactaa tttattcgct tcatcgaaag gtttcgtgcc tggcggtact
    19381 tcgtcataca taattttgta gccgaagccc atgccaccga caaaactttt aaacttactg
    19441 gaatcttcga gggtaatatt tcctgtcgtg agattgactc ttaatatatt acctgtccaa
    19501 ccgttagcca tgattttttc ctttgcaaga ttctcacagt aatatctttc cactcgataa
    19561 tttttaacgc ccctgttggg caggcgtttg cacattcacc gcataacaca catttcgagg
    19621 attttttact ttcggtattt acggtggcca tcatccacgg gcatgccgta gtacaggcgc
    19681 tacagccaat accgcgttta tgatcgacgg taatacagcc ttctttctgc tgccaggtaa
    19741 tcgcaccaat cgggcagacg ttcatgcatt gcggctcttt gcattgacgg caggtgtccg
    19801 cggtatagtt gagatcgcca tacaggccgc cgccagagcc aaccccgttg tcgccaaaga
    19861 aataattgcg atggattttg atacgggaga agaatgtccc tactgagcca tcgttgaagt
    19921 tggtacaaga gatttcacag cggtggcaac ccgtgcatcg cgctcgctgg gtcaccagca
    19981 cccctttggg tgtatttatc aaccctactg tgccgctatc aatatcttcc tgtttgcaac
    20041 ccaaaagtga cagtaacgca ggggcaattg ttagaccagc aaggcctttt cctgatatac
    20101 gcaagaattc taatcgcgtt aagccaatat ctaatagtgg acgatcaacc gggttcattt
    20161 atttcgcatc ctcttcgcaa acacagtgct gacaattcag cagcctgata ttaagcattc
    20221 aattaatacg ctgctgactc tgtccccaac accagatatt ttccctaacc cgatagtgta
    20281 tgagaaaaga tttaacgccg ataaactaat cctttatggt taggagaaaa aataatgtga
    20341 ttattctgag cccttaacat tgatcgttat caattaaaat tacaaagcaa gaaattataa
    20401 gtga
//
LOCUS       DADLFA010000064        20113 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000064 DADLFA010000000
VERSION     DADLFA010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20113
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.064"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..1985
                     /locus_tag="JQH65_001945"
     CDS             <1..1985
                     /locus_tag="JQH65_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HAY6139367.1"
                     /translation="VGAFLAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDT
                     FNTEQVEVIKGPSGTDYGHSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVN
                     QVIGDTTAVRLNVMGEKTHDAGRDKVKNERYGVAPSVAFGLGTANRLYLNYLHVTQHN
                     TPDGGIPTIGLPGYSAPSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDI
                     NDNTTIRNTTRWSRVKQDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILT
                     NQTNLTSTFYTGSIGHDVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTR
                     NGANANGQTDTFAIYAFDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCP
                     AGVAKGSPVTTVDTAKSGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQS
                     GSGNSANRTDFKPQKANTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQY
                     GKKRVEGYEISVAGNITPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLW
                     SQYQATDDISVGAGARYIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLN
                     VYNLFDTDYVASINKSGYRYHPGKPRTFLLTANMHF"
     gene            1991..2704
                     /gene="ybiX"
                     /locus_tag="JQH65_001946"
     CDS             1991..2704
                     /gene="ybiX"
                     /locus_tag="JQH65_001946"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706682.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HAY6139368.1"
                     /translation="MWGAGPTFWRNCMMYHIPGVLSPQDVARFREQLEQAEWVDGRVT
                     TGAQGAQVKNNQQVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNN
                     ETYGFHVDGAVRSHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGD
                     LVLYPSSSLHCVTPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENE
                     EILSLLNLYHNLLREWSEI"
     gene            2778..3044
                     /gene="ybiI"
                     /locus_tag="JQH65_001947"
     CDS             2778..3044
                     /gene="ybiI"
                     /locus_tag="JQH65_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HAY6139369.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            3309..3569
                     /locus_tag="JQH65_001948"
     CDS             3309..3569
                     /locus_tag="JQH65_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY6139370.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLTEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(3797..4882)
                     /locus_tag="JQH65_001949"
     CDS             complement(3797..4882)
                     /locus_tag="JQH65_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate/lactate/ureidoglycolate dehydrogenase"
                     /protein_id="HAY6139371.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(5023..5985)
                     /gene="ybiB"
                     /locus_tag="JQH65_001950"
     CDS             complement(5023..5985)
                     /gene="ybiB"
                     /locus_tag="JQH65_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HAY6139372.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLEATGEAATISDGLARVNQAF"
     gene            complement(6013..8163)
                     /gene="dinG"
                     /locus_tag="JQH65_001951"
     CDS             complement(6013..8163)
                     /gene="dinG"
                     /locus_tag="JQH65_001951"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HAY6139373.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            8283..8765
                     /gene="ybiA"
                     /locus_tag="JQH65_001952"
     CDS             8283..8765
                     /gene="ybiA"
                     /locus_tag="JQH65_001952"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HAY6139374.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRALLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            complement(8997..10361)
                     /gene="rhlE"
                     /locus_tag="JQH65_001953"
     CDS             complement(8997..10361)
                     /gene="rhlE"
                     /locus_tag="JQH65_001953"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HAY6139375.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSSDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            10590..11261
                     /gene="cecR"
                     /locus_tag="JQH65_001954"
     CDS             10590..11261
                     /gene="cecR"
                     /locus_tag="JQH65_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HAY6139376.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNTTTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            11261..12259
                     /gene="hlyD"
                     /locus_tag="JQH65_001955"
     CDS             11261..12259
                     /gene="hlyD"
                     /locus_tag="JQH65_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HAY6139377.1"
                     /translation="MMKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLKLYGNVDIRT
                     VNLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQVKAGVSVAQAQYDLML
                     AGYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQA
                     TLKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTR
                     AVEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQ
                     IGFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            12252..13988
                     /locus_tag="JQH65_001956"
     CDS             12252..13988
                     /locus_tag="JQH65_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY6139378.1"
                     /translation="MNDAVITLNGLEKRFPGMGKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATMIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELASEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            13981..15114
                     /locus_tag="JQH65_001957"
     CDS             13981..15114
                     /locus_tag="JQH65_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY6139379.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            15125..16231
                     /locus_tag="JQH65_001958"
     CDS             15125..16231
                     /locus_tag="JQH65_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY6139380.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIATNYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(16193..16603)
                     /locus_tag="JQH65_001959"
     CDS             complement(16193..16603)
                     /locus_tag="JQH65_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6139381.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            16736..17487
                     /locus_tag="JQH65_001960"
                     /pseudo
     CDS             16736..17487
                     /locus_tag="JQH65_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
     gene            17484..18725
                     /gene="clsB"
                     /locus_tag="JQH65_001961"
     CDS             17484..18725
                     /gene="clsB"
                     /locus_tag="JQH65_001961"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HAY6139382.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNKLTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIEPHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWHPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            18791..19681
                     /locus_tag="JQH65_001962"
     CDS             18791..19681
                     /locus_tag="JQH65_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HAY6139383.1"
                     /translation="MIVLLVVYAKKVDWEEVWKVIRDYNRVALLSAVGLVVVSYLIYG
                     CYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTWVGGIGMRYRLYSRLGLPGSTIT
                     RIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVAQTTLRILGIGLLMIIAVYLWFC
                     AFAKHRHMTIKGQKLVLPSWKFALTQMLISSVNWMVMGAIIWLLLGQSVNYFFVLGVL
                     LVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGSIIAALLAYRVLYYFIPLLLALI
                     CYLLLESQAKKLRAKNEAAM"
     gene            complement(19717..>20113)
                     /locus_tag="JQH65_001963"
     CDS             complement(19717..>20113)
                     /locus_tag="JQH65_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BAX inhibitor (BI)-1/YccA family protein"
                     /protein_id="HAY6139384.1"
                     /translation="IIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVII
                     VNAALMSERPIWVISCLVIVLWPGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALT
                     LYYYFIGFFGILAAIAITLVWQRHTRFFH"
BASE COUNT         4722 a         5447 c         5301 g         4643 t
ORIGIN      
        1 gcgtgggtgc gtttttggcg ggtgagaacg gtaactccac cactggcgac gccatttata
       61 tgcgtggtgc cgatacctct aacagtattt atattgatgg cattcgcgat atcggcagcg
      121 tctcgcgcga caccttcaat accgagcagg tcgaagtgat taaagggccg tccggcaccg
      181 actacgggca cagcgcaccg acgggctcga tcaatatgat cagcaaacag ccgcgcaatg
      241 attccggcat tgacgcctcc gccagtattg gcagcgcctg gttccgccgc ggcacgctgg
      301 acgtcaatca ggtcattggt gataccaccg cggtgcgcct gaatgtaatg ggcgaaaaaa
      361 cgcacgatgc cggacgcgac aaagtcaaaa atgagcgtta cggcgtcgcc ccttctgtcg
      421 cttttggcct tggtacagcg aatcgtttgt atcttaatta tctgcatgtc acccagcaca
      481 acacgccaga cggcggcatt ccgaccatcg gtttgccggg ctattctgcc ccatctgcgg
      541 gaacggcggc cctgaatcat tccggaaaag ttgatactca taacttttac ggcacggatt
      601 ccgattacga cgattcgacc accgacaccg ccaccatgcg ttttgagcac gacatcaacg
      661 ataacaccac cattcgcaat actacccgtt ggtcgcgcgt aaagcaggat tacctgatga
      721 cggcgattat gggcggggcg tcgaatatta ctcagcccac cagcgatgtg aatagctgga
      781 cctggtcacg cacggcgaat accaaagatg tgagtaataa aattctcacc aaccagacca
      841 acctgacctc gacgttctat accggttcta tcggtcatga tgtcagtacc ggcgtggaat
      901 ttacccgtga aacgcagact aactacggcg ttaatccggt gacgttaccc gcggtaaata
      961 tttatcatcc tgacagcagc attcatcccg gcggcctgac gcgcaacggc gcaaacgcca
     1021 atggtcagac ggataccttc gcaatttacg cctttgatac gctgcaaatc acccgtgatt
     1081 ttgagctgaa cggcgggatc cgtctggata attatcatac tgaatatgac agtgccaccg
     1141 cctgcggcgg cagcggacgc ggtgccatca cctgcccagc tggtgtggca aaaggttctc
     1201 cggtcaccac cgtcgacacc gccaagtcgg gcaatctggt gaactggaaa gccggggcgc
     1261 tgtatcacct gacggaaaac ggtaatgtct atattaacta tgccgtttcc cagcagcctc
     1321 cgggcggcaa caacttcgcc cttgcgcagt ctggcagcgg taacagtgcc aaccgcacag
     1381 attttaaacc gcaaaaagcc aacaccagcg agattggcac caaatggcag gttctggata
     1441 aacgcctgtt gctcaccgcc gcactgttcc gtactgatat cgaaaatgaa gttgagcaaa
     1501 atgatgacgg aacttactcg caatacggta agaaacgtgt cgaaggctat gagatatccg
     1561 tggccgggaa tatcactccc gcgtggcagg tgattggcgg ctatacccag caaaaagcaa
     1621 ccatcaaaaa cggcaaagat gttgcccagg atggttcctc atcgctgccg tataccccgg
     1681 agcacgcctt caccttatgg agccagtatc aggcaaccga tgatatctct gttggcgcgg
     1741 gcgcacgcta tatcggcagt atgcataaag gttcagacgg cgcggtggga acgccagcgt
     1801 ttaccgaagg ttactgggtc gccgatgcca aactggggta tcgggttaat cgcaatctcg
     1861 acttccagct aaacgtttac aacctgtttg ataccgatta cgtcgcctca atcaacaaga
     1921 gcggctaccg ttatcacccg ggcaagccaa gaaccttctt gctcacagcc aatatgcatt
     1981 tctgattcag atgtggggcg caggccccac tttttggaga aattgtatga tgtaccacat
     2041 tcccggcgtg ttatcgccac aggacgtcgc tcgttttcgc gaacaactgg aacaagccga
     2101 atgggtggat ggacgcgtca ccaccggcgc acaaggtgcg caagttaaga acaatcaaca
     2161 ggtagacacc cgcagcacgt tatacgccgc cctgcaaaat gaggtgctga acgcggttaa
     2221 ccaacatgct ttattctttg ccgcggcctt gccgcgtacc ctttccacgc cgctgtttaa
     2281 tcgctatcag aacaatgaaa cctatggttt tcatgtggat ggcgcagtac gcagccatcc
     2341 acaaaacggc tggatgcgca ctgacctttc tgccacgctg tttttaagcg atccacaaag
     2401 ctacgacggc ggcgaactgg tcgttaatga caccttcgga caacatcggg taaaactccc
     2461 ggcaggtgat ctcgtgttgt atccctccag cagcctgcat tgcgtgacac ccgtaacccg
     2521 cggcgtacga gtggcatcat ttatgtggat ccagtcgatg atccgcgatg ataaaaagcg
     2581 cgccatgctg tttgaactag acaaaaatat tcagtcgctg aaaagccgct atggtgaaaa
     2641 tgaagagatc ctgtcgctgc tcaatcttta tcataatctg ctgcgggaat ggtcggagat
     2701 ctgacgcttg aactgcccag gttcgtccct atattagcta cacttaactg tacaagcatt
     2761 gatatgggga ggacgatatg gcatccggtt gggctaacga tgacgccgtc aacgaacaga
     2821 tcaacagtac aattgaagat gcgattgccc gcgctcgggg tgaaattccg cgtggcgaaa
     2881 gcctgtatga atgtgaagag tgcggtgccc ccatcccgca agcccgtcgg gaagccattc
     2941 ctggcgtgcg cttatgtatt cattgtcagc aggagaaaga tttacaaaaa ccagcgtata
     3001 caggatataa tcgcagaggt tcgaaagaca gccagttacg ttaactacaa ctggcgagat
     3061 gcatagcgag tataatttgt attttgcgtg gcattccgtg cctttaacgc tgctgtgact
     3121 gccacgcggc ataaaacaag tccataagta caacaaataa atggtttatc agtatgttag
     3181 atgtcaatca atttatttga acaaggcggt caattctctt cgattttatc tctcgaaaaa
     3241 aaacgtgata ctcatcacat cgacgaaaca acgtcactta tacaaaaatc acctgcgaga
     3301 gattaattat gaaaactatc aatactgttg ttgctgctat ggctctttca actctatcat
     3361 ttggcgtgtt cgcggcggaa ccggtaacgg catcccaggc acagaacatg aataaaatcg
     3421 gcgtggtttc tgccgatggc gcatccaccc tcgatgccct ggaagcgaaa ctgactgaga
     3481 aagccgcggc agccggtgcc agtggataca gcatcacttc tgccaccaac aacaataaat
     3541 taagcggtac tgcggtaatt tataagtaat tcgtaggacg gataaggcgt ttacgccgca
     3601 tccggcaaca gatgcctgat gcgacgctac cgcgtcttat caggcctaca aaatccgaac
     3661 cgttggacgg atcaggcggt tacgctacgt ccagcacaag tatttccctg tctgtttgcc
     3721 gacagacgca tatgctctaa ccctcattga tcctatatta cccttgtttg cccgtccgtt
     3781 actggacggg ctttttttag ctggctaact gctgacagaa agcctgcaac gtctcttccg
     3841 acataccaat ctgccgcgcc gcatcacaaa tggcctgcca gcttcccgca tccaatggaa
     3901 tcccctgctc ctgccgttcg cgacgcgtgt tcacttccca ctcgcccggt agcaaaatcg
     3961 gcttatcgtc atcatgcggc gaggctttca cccactcggc aaaggcttca gtctgcgcgc
     4021 tacaatccgg cgcgccgaac agttccgggt tgatgatgat agtggtcatg cagttaagaa
     4081 tggcatcggg actggtttgt aacgtttcct gatgcgtcgt tttaccgcca gaaagcgccc
     4141 cgccaagaat ttcacacatc gctgcaaggg cgtagccttt atgttcggca aaggtcaaca
     4201 gcgaccccaa cggcgactcc tgcattaccg ccggattggt cgtcggcacg ccgttaacgt
     4261 caatcaggca acctggcggc acggggacgc ctttatgcca ggcgacgcga gttttaccaa
     4321 atgcgatggc gctggtggcg taatcaagca acagcgggaa attatcttta cgagggaaaa
     4381 ccacacagaa cggattggtg ccaaagcggc tgtcgcgacc gtggaacggt gcgaccattg
     4441 gaataccgac cacgctaaca aagtggatag agacaaaccc cgccgctgca cactgctccg
     4501 cccagtaacc gatacggccg atatgatgcg agttatgtag cgccacggca gcaataccgt
     4561 gctgatgcgc tttctcaatc cccagcgcca tcgcttcgtg tgccgcgacc tgaccgaatg
     4621 cgcgatcgcc atcgagcgtg actgccgccc ccgcctcttt aacgattttg gcatgatggt
     4681 taatttgcag atgcccctga ctccaggaac gcacatagct tgggatcatg ccaataccat
     4741 gagaatcatg ccctgccagg tttgccgcga ttaaatgatc ggccactaat ttcgcttctt
     4801 gttcctcgct acccatctga cgaaatacag cctgaataaa actgtgcagc gtctgagcat
     4861 caaagcgatg accactttcc atggttaact cctgtttatg ttatgtgttt gttgtttttt
     4921 tatgctgcac gccgggcgcg ggaaggtcaa taaagatgca ggaatttcat attgcggtgg
     4981 ggtaaaagcc ggatgacatg gctcatccgg ctgagaaaag aattaaaatg cctgattaac
     5041 gcgcgccagg ccgtcgctga tagttgccgc ttcacccgta gcctccaggc agcaagccat
     5101 ctggattttc agcgattcgg gaatcggttc gctgccagca aggcaacgct caatccactg
     5161 cgccgtggtt tctgggtctt ttgcttgtgg cagtaactcg ctaccagcag tgtcctgttt
     5221 ttcatacagc acccgcatcc cttcacggtc aatgagattg atctgcgggc agcgctgcgg
     5281 attagcatac acttcgcctt cagtaccatg cattagtagc gcccgcccgc cgatatcgct
     5341 aaagaacttc gcgactcgcc cgatgtattc cggatgcgaa acgctggaaa gacgcagcgc
     5401 ctcaccttcg gcaaatggcg tcgccagttt cgccagggta tgtgcactgt tacgcacgcc
     5461 catccgccag cgcatcgcca gttgtttttc cagcggcggg caaaacgcgc cgacaggcat
     5521 aaacaccggt tgatgttcgt cgagcttcgc ctgcgcctgc ccgccgtgca acgttggtgt
     5581 aatacccatc agttcgaaaa tggtttctgt cagcacgcga gttggatctt cgctaacccc
     5641 gtgaaccacc acaggaaaac cgagtttgtg caggagaata gccaacaacg gcgtcaggtt
     5701 ggcctgttta cgcgcgccgt tgtaactggg gatgacaatc ggcatcggct tgcctgcagg
     5761 cggcgtcagc ttgatggtgt gattttgcat ggcttcgtaa aagccgagca tctctgcttc
     5821 cccttcccct ttgatacgta gcgcaatcaa tacgccgccc aactcaaggt cagggacttc
     5881 accattgagc atatgggcat acagaccgcg cgcagtatcc cggtctaaat cgcgcgcgtg
     5941 gtttttcccg cgcccgatct ctttaatgat tttgcgatag tccatttacg actccttacc
     6001 tgactcacat cattaacgcc gccgacggcg tggggatttc gttttttctt tctttttaac
     6061 tataccttca gggacttccg gttgctctat cggaaatacc ggtaatgcat ccagtagtcg
     6121 cttgccataa tttttggtca gcaagcgttt gtcgtagata accacttcgc cccagcaacc
     6181 gtggcttcga atcagtcgcc caacctgctg aatcaggtta aacgaggcgc tcggcaggct
     6241 ttgcacctca aacggatagc ggttgaggct tttcagccat tccccttcgg tgatcaccac
     6301 cgggctgtcg atgggcggaa aagcgatttt gtggatatgc acctggctga gcagatcacc
     6361 tttcaaatca agcccttcgg caaatgactg taagcccacc agcacactac gctcaccgtt
     6421 ggcaacgcgt ttgcggtgca gttcaactaa gcgataacgc ggctgatcgc cctgcaccag
     6481 cagcatcagg cgtaaatccg tcacatagtc gagaaagcgc tgcatcgccc gtccgctggc
     6541 aaacagtacc aacataccga gatgtttttt gctctccacc tgctcacgga aaaaggccgc
     6601 catttcggca atatgctgct cttcgttgtc gatggaaggc tcaaagcgca tccggggaat
     6661 aacaattttg ccctgttcgc agtggttaaa gggggaatcc agcgccacaa aacggtcgcc
     6721 cgctttctct ttcagcccgc tcatctcctg caaacgcgaa aaactgttca gcgaacgcaa
     6781 ggttgcggag gtgacaataa tgtgcggaat actgcgccac agcagccttt ccagctgatc
     6841 gctgacacgt attcccacgc agtgaaacca gagatgcagc tgcccttcgc gctcttcccg
     6901 cgtcgcccat ttggtcaccg gcgcaccgga agattgcgcc agagacgcca gccgccagag
     6961 tttgctttgc gcctcgaaca tccccaacgc gcggttcatc tgcaaaatca accgatgcag
     7021 acgcacaata tcgtggctgc cggttttctc acttaaatcg ttaagaaata actccgccag
     7081 accacgcagc atctcggtga gttttgccag ccgctggcag atctccagca cttcatcagg
     7141 cagttcgccc atcgcaaaac ggtgctctgc ctcctgcccg gcaggcatgt agagattgag
     7201 aatgttgttt aacgaggcga taagctcata caactcttca caatgcgcat tcaaacgttc
     7261 agggatcgcc agcggtggga tggtcttcgg gcgaaactgc tccatgcagg tagcgaccag
     7321 tttcgtgaac aagtccagct gtagtcgata ccagggcgcg gtgatttcag cgctcatctc
     7381 cagcgcatcc cgggccacat ctggcaggtg atgaccttcg tccagcacca gcagcagatt
     7441 tttcgggtca ggcagtacgg cttcgctttc catcgccgcc atcaccagcg catggtttgc
     7501 caccaccact tccgcttcct gaatctcccg acgagcaaca aaaaacgggc attcacgata
     7561 gtagtaacag ttacggttaa ggcagctggc tttgtcggta ctcaaacgac gccagagatc
     7621 atcatctata gcgatatccg tatgatcgcg cagaccatcc catttataag tgtcgagatc
     7681 gcccttcagc ttcgcacaac gtttttgctc ttcctgatta tttggcgtca gttcgtcgtc
     7741 aagaaacgcc agcagatcct gttgcgtggg ttcagtgctg gcgagcgccg tcagattacg
     7801 cggacaaacg tagcgcccac gcccgaaagc ggcagtgaat ttaagatcgg gaatgatctt
     7861 tttcagcagc ggtaaatctt tgctgtaaat ctgatcctgc aacgccacgt tggcggtact
     7921 caccaccagc gttttttgct cttcgcgggc aatggcgatg ccggggatca aataggagag
     7981 cgttttccca acgccggtgg gggcttcaat cgccagatgc cgcccttctt ctccggccag
     8041 cgttttggcg acgtccgcaa tcatctgccg ctgcggcgca cggggaataa agtcggggat
     8101 ctgttcctga agcgccttat accaggcggc aatttgcgct ttaagcgcgg cggttaatgc
     8161 catgagaaaa cctgaaatac tgtataaaca gccaatattg tggcattttt cggttttcag
     8221 gggtaatggt ttttctgttt ctggaaagcg gctcgcaata accaccaaca taatgcggtt
     8281 taatgcccgt tcgagcacaa aggatccaac acgtcatgca agacaccatc ataaatttct
     8341 acagcaccag cgatgactac ggtgattttt ctaactttgc cgcctggccc atcaaggttg
     8401 acgggaaaac ctggcccacc tccgaacact attttcaggc acaaaaattc cttgatgaaa
     8461 aataccgcga agagatccgt cgggtttctt cccccatggt cgccgctcgc atggggcgta
     8521 atcgttccaa gccacttcgt aaaaactggg agtcggttaa agaacaggtg atgcgtaaag
     8581 cacttcgtgc taaattcgaa cagcatgcag aactgcgtgc gctcttgctg gcaaccgcgc
     8641 ccgcaaaact ggttgagcat acggaaaacg atgcttactg gggagacggt ggtaatggta
     8701 agggcaagaa tcgactgggc taccttttaa tggagttgcg cgaacaattg gctatagaga
     8761 agtaacgtct cggtggctta tgggcggtaa aacagataaa ttttttcccg tgttctcaaa
     8821 ttgctgacaa gtgcttgttg tgaaatgccg gatgcggcgt aaacgcctta tccggcctac
     8881 aaaagcatgc aaattcaata tattgcagag ttaacgtagg cctgataagc gtagcgcatc
     8941 aggcagtttt gcgtttgttc atcagcctga tgccgggcat agcccggcat aaaagattac
     9001 tgcgcagcgg caggtttacg cggacggcgg cgacgttgtt gttcgcctgc cggtttggca
     9061 tcgccgaggc ggcgagacgg tttttctgca ggtttcgcgc ttgcagattt tgcgccacct
     9121 tccccacggc gtggttgctg ttgaccgcga ccaccacctt gcccacgacc gccgccgcca
     9181 cgttgctggc gaccgttctg gatcggttcg gctttgattg acgggtccgg ctcatagccc
     9241 ggaatcgcaa tgcgcgggat ctcttttttc agcagttttt cgatatcacg cagcagtttg
     9301 tgttcatcaa cacacaccag cgacaacgct tcaccggtag cagccgcacg accggtacgc
     9361 ccgatacggt ggacataatc ttccggtacg tttggcagtt catagttgac gacgtgcggc
     9421 aactcttcga tatccagacc gcgcgcagcg atatcggtcg ccaccagtac gcgaatgtca
     9481 ctcgatttaa aatcagccag cgcacgggta cgcgcacctt gcgatttatt cccgtggatc
     9541 gccgcactac ggatgccatc tttattgagc tgctcagcca gatggttagc gccgtgtttg
     9601 gtacgggtaa acaccagcac ctgctgccag ttccctttac caatcatgtg cgacagcaat
     9661 tcgcgtttgc gtttcttatc gacaaagtga acgtgctggg tcacctgatc agacgcggta
     9721 ttgcggcgtg ccacttcgat ttccagcggg ttgtgcaaca gtttttccgc cagggcttta
     9781 atatcgtcag agaaggtcgc agagaacagc aggttctggc gtttcgctgg cagttttgtt
     9841 aacacgcgac ggatatcgtg gataaagccc atgtcgagca tgcggtcagc ttcatcgagg
     9901 acgaggattt caacctgatc cagcttcact gcattttgat gttccaggtc cagcaaacgt
     9961 cccggtgttg ccaccagaac atcaacgccg ccgcgcagct tcatcatttg cgggttaatg
    10021 ctgacaccgc caaacaccac cagcgaacga atgttcaaat atttgctgta atcacggacg
    10081 ttttcgccaa tctgcgcagc cagttcacgg gtcggggtaa gaatgagcgc acgtaccgga
    10141 cgacgccctt tggcgtgcgg ctggcgagtg atcaggtgtt gcaacagcgg cagcgtaaag
    10201 cccgctgttt taccggtacc ggtctgggcg ctagccatca ggtcgcggcc ttccagcacc
    10261 gcagggatcg cctgctgctg aataggggtg ggttcacggt aaccctgctc ggcaacggcg
    10321 cgcaggatat cagggcttaa acccaaagaa tcgaaagaca taactactcc gaaccgcccc
    10381 gaccgttacc ggtgtagttt tcagggagat actgaaaagg aaaatgacaa aaaccacaat
    10441 gtcatgaggg agcggagtgt agcagttttt gtgacgcagc gcataaatta tccctgtgac
    10501 tggcgttgct aaaattctta gccatactgg acaactcccc tatggagtca taatcttaat
    10561 caatcatttg attaagaatg gactgtgcga tgaataatcc tgccatgaca atcaagggtg
    10621 aacaggcgaa aaaacagctg attgctgccg cactggcgca gtttggtgaa tatggaatga
    10681 acaccaccac tcgcgagata gccgcccagg ccgggcagaa tatcgctgcc atcacctact
    10741 acttcggttc gaaagaagat ttgtacctcg cctgcgccca gtggattgcc gattttattg
    10801 gcgagcagtt ccgtccgcat gccgaggaag ccgaacgctt gttcgcgcaa ccacagcctg
    10861 atcgggctgc catccgtgaa ctgatccttc gcgcctgcag gaacatgatt aagctgctca
    10921 cccaggatga caccgtcaac ctcagcaagt ttatctcccg tgagcagctc tctcccacgg
    10981 cagcctacca cctggtgcac gaacaggtga ttagtccgct acacagccac ctgacacgcc
    11041 tgattgccgc ctggaccggc tgcgacgcca atgacacccg catgatcctt catacccatg
    11101 cgttgattgg tgagattctg gcgttccgtc ttggcaaaga aacaattctg ttacgtaccg
    11161 gctggaccgc gttcgatgaa gaaaagaccg aactgatcaa ccagacggtg acctgtcata
    11221 tcgacctgat tttgcaagga ttatcgcaaa ggagtttgta gtgatgaaaa aacctgtcgt
    11281 gatcggactg gcggtagtgg tacttgccgc cgtggttgcc ggaggctact ggtggtatca
    11341 aagccgccag gataacggcc tgaagctgta tggcaacgtg gatattcgta cggtgaatct
    11401 tagtttccgt gttggggggc gcgttgaatc gctggcggtg gacgaaggtg atgcgatcaa
    11461 agcgggccag gtgctgggcg aactggatca caagccgtat gagattgccc tgatgcaggt
    11521 gaaagcgggc gtctcggtgg cacaggcgca gtatgacctg atgcttgccg ggtatcgcga
    11581 tgaagaaatc gctcaggccg ccgcagcggt gaaacaggcg caagccgcct atgactatgc
    11641 gcagaacttc tataaccgcc agcaaggact gtggaaaagc cgcaccattt cggcaaatga
    11701 cctggaaaat gcccgctcct cgcgcgacca ggcacaggca acgctgaaat cagcacagga
    11761 taaattgcgt cagtaccgtt ccggtaaccg tgaacaggac atcgctcagg cgaaagccag
    11821 cctcgaacag gcgcaggcgc aactggcgca ggcggagctg aatttacagg actcaacgtt
    11881 gatagcaccg tctgatggca cactgttaac gcgcgcggtg gagccaggca cggttctcaa
    11941 tgaaggtggc acggtgttta ccgtttcact aacgcgtccg gtgtgggtgc gcgcttatgt
    12001 tgatgaacgg aatcttgacc aggcccagcc ggggcgcaaa gtgctgcttt ataccgatgg
    12061 tcgcccggac aagccgtatc acgggcagat tggtttcgtt tcgccgactg ctgaatttac
    12121 cccgaaaacc gtcgaaacac cggatctgcg taccgacctc gtctatcgcc tgcgtattgt
    12181 ggtgaccgac gccgatgatg cgttacgcca gggaatgccg gtgacggtgc aattcggtaa
    12241 cgaggcagga catgaatgat gccgttatca cgctgaacgg cctggaaaaa cgctttccgg
    12301 gcatgggcaa gcccgccgtc gcgccgctcg attgtaccat tcacgccggt tatgtgacgg
    12361 ggctggtggg gccggatggt gcaggtaaaa ccacgctaat gcggatgctg gcgggattac
    12421 tgaaacccga cagcggcaat gccacgatga ttggctttga tccaatcaaa aacgacggcg
    12481 cgctgcacgc cgtgctcggc tatatgccgc agaaatttgg tctgtatgaa gatctcacgg
    12541 tgatggagaa cctcaatctg tacgcggatt tgcgcagcgt caccggcgag gcacgtaagc
    12601 aaacttttgc tcgcctgctg gagtttacgt ctcttgggcc gtttaccgga cgcctggcgg
    12661 gcaagctctc cggtgggatg aaacaaaaac tcggtctggc ctgtaccctg gtgggcgaac
    12721 cgaaagtgtt gctgctcgat gaacccggcg tcggcgttga ccctatctca cggcgcgaac
    12781 tgtggcagat ggtgcatgag ctggcaagcg aagggatgtt aatcctctgg agtacctcgt
    12841 atctcgacga agccgagcag tgccgtgacg tgttactgat gaatgaaggc gagttgctgt
    12901 atcagggaga accaaaagcc ctgacacaaa ccatggccgg acgcagcttt ctgatgacca
    12961 gtccacacga gggcaaccgc aaactgttgc aacgcgcctt gaaactgcca caggtcagcg
    13021 acggcatgat tcaggggaaa tcggtacgtc tgatcctcaa aaaagaggcc acaccagacg
    13081 atattcgcca tgccgacggg atgccggaaa tcaacatcaa cgaaactacg ccgcgttttg
    13141 aagatgcatt tattgatttg ctgggtggtg ccggaacctc agaatcgccg ctgggtgcaa
    13201 tattgcatac ggtagaaggc acacccggcg agacggtgat cgaagcgaaa gaactgacca
    13261 agaaatttgg tgattttgcc gccaccgatc acgtcaactt tgccgttaaa cgtggggaga
    13321 tttttggttt gctggggcca aacggcgcgg gtaaatcgac cacctttaag atgatgtgcg
    13381 gtttgctggt gccgacttcc ggccaggcgc tggtgctggg gatggatctg aaagagagtt
    13441 ccggtaaagc gcgccagcat ctcggctata tggcgcaaaa attttcgctc tacggcaacc
    13501 tgacggtcga acagaattta cgctttttct ctggtgtgta tggcttacgc ggtcgggcgc
    13561 agaacgaaaa aatctcccgt atgagcgagg catttggcct gaaaagtatc gcctcccacg
    13621 cgaccgatga actgccatta ggttttaaac agcggctggc gctggcctgt tcgctgatgc
    13681 atgaaccgga cattctgttt ctcgacgaac caacgtccgg cgttgatccc ctcacccgcc
    13741 gcgaattttg gctgcatatc aacagcatgg tagagaaagg cgtcacggtg atggtcacca
    13801 ctcactttat ggatgaagca gaatattgcg accgcatcgg cctggtgtac cgtgggaaat
    13861 taattgccag cggcacgccg gatgatttga aagcgcagtc ggccaacgac gagcaacccg
    13921 atcccaccat ggagcaagcc tttattcagt tgatccacga ctgggataag gagcatagca
    13981 atgagtaacc cgatcctgtc ctggcgtcgc gtacgggcgc tgtgcgttaa agagacgcgg
    14041 cagatcgttc gcgatccgag tagctggctg attgcggtag tgatcccgct gctactgctg
    14101 tttatttttg gttacggcat taacctcgac tccagcaagc tgcgggtcgg gattttactg
    14161 gaacagcgta gcgaagcggc gctggatttc acccacacca tgaccggttc gccctacatc
    14221 gacgccacca tcagcgataa ccgtcaggaa ctgatcgcca aaatgcaggc ggggaaaatt
    14281 cgcggtctgg tggttattcc ggtggatttt gcggaacaga tggagcgcgc caacgccacc
    14341 gcaccgattc aggtgatcac cgacggcagt gagccgaata ccgctaactt tgtacagggg
    14401 tatgtcgaag ggatctggca gatctggcaa atgcagcgag cggaggacaa cgggcagact
    14461 tttgaaccgc ttattgatgt acaaacccgc tactggttta acccggcggc gattagccag
    14521 cacttcatta tccccggtgc ggtgaccatt atcatgacgg tcatcggcgc gattctcacc
    14581 tcgctggtgg tggcgcgaga atgggaacgc ggcaccatgg aggctctgct ctctacggag
    14641 attacccgca cggaactgct gctgtgtaag ctgatccctt attactttct cgggatgctg
    14701 gcgatgttgc tgtgtatgct ggtgtcagtg tttattctcg gcgtgccgta tcgcgggtcg
    14761 ctgctgattc tgttttttat ctccagcctg tttttactca gtaccctggg gatggggctg
    14821 ctgatttcca cgattacccg caaccagttc aatgccgctc aggtcgccct gaacgccgct
    14881 tttctgccgt cgattatgct ttccggcttt atttttcaga tcgacagtat gcccgcggtg
    14941 atccgcgcgg tgacgtacat tattcccgct cgttatttcg tcagcaccct gcaaagcctg
    15001 ttcctcgccg ggaatattcc agtggtgctg gtggtaaacg tgctgttttt gatcgcttcg
    15061 gcggtgatgt ttatcggcct gacgtggctg aaaaccaaac gtcggctgga ttagggagaa
    15121 gagcatgttt catcgcttat ggacgttaat ccgcaaagag ttgcagtcgc tgctgcgcga
    15181 accgcaaacc cgcgcgattc tgattttacc tgtgcttatt caggtgattc tgttcccgtt
    15241 cgccgccacg ctggaagtga ctaacgccac catcgccatc tacgatgaag ataacggcga
    15301 gcattcggtg gagctgaccc aacgttttgc ccgcgccagc gcctttactc atgtgctgct
    15361 gctgaaaagc ccacaggaga tccgcccaac catcgacaca caaaaggcgt tactcctggt
    15421 gcgtttcccg gctgacttct cgcgcaaact ggataccttc cagaccgcgc cgttgcagtt
    15481 gatcctcgac gggcgtaact ccaacagtgc gcaaattgcc accaactacc tgcaacagat
    15541 cgtcaaaaat tatcagcagg aactgctgga aggaaaaccg aaacctaaca acagcgagct
    15601 ggtggtacgc aactggtata acccgaatct cgactacaaa tggtttgtgg tgccgtcact
    15661 gatcgccatg atcaccacta tcggcgtaat gatcgtcact tcactttccg tcgcccgcga
    15721 acgtgaacaa ggtacgctcg atcagctact ggtttcgccg ctcaccacct ggcagatctt
    15781 catcggcaaa gccgtaccgg cgttaattgt cgccaccttc caggccacca ttgtgctggc
    15841 gattggtatc tgggcgtatc aaatcccctt cgccggatcg ctggcgctgt tctactttac
    15901 gatggtgatt tacggtttat cgctggtggg attcggtctg ttgatttcat cactctgctc
    15961 gacacaacag caggcgttta tcggcgtgtt tgtctttatg atgcccgcca ttctcctttc
    16021 cggttacgtt tcgccggtgg aaaacatgcc agtatggctg caaaacctga cgtggattaa
    16081 ccctattcgc cactttacgg acattaccaa gcagatttat ttgaaggatg cgagtctgga
    16141 tattgtgtgg aatagtttgt ggccgctact ggtgataacg gccacgacag ggtcagcggc
    16201 gtacgcgatg tttagacgta aggtgatgta acttcttatc tttcgccagc aaagacacta
    16261 ccgccgggcc ggcaaggatt gccagccctg caattgccag caaaaagttg ttttccagta
    16321 cgcgggacaa cagccacagc acaatcagcg ctgcgccaaa ataccaggtc gtggtcagct
    16381 cttccaacac gtctgccacc tcacgccagc gttgctcggg gtggcgctga aacaccaaca
    16441 tcatcactac cgtgacacaa tacagtgcgc ataatcccac gccgacgtga accagagtct
    16501 tactcatcca gcccaccacc agcagcgcca ctaccagtaa atgcaacata atctgccagc
    16561 aacttagacc cgttgcgaca cgaacacgtt gttgccactt catggcttct ctcctgaaga
    16621 atttatctct acttactcag agtaccgaac tttacggaaa attccgtaag accgcacctt
    16681 tttgtgacgc cgactacact atttcaatga gataagaggc aaagacagga aaattatgcc
    16741 cgatcaaaca caacaatttt cgttcaaggt gctcaccatc aacattcaaa aaggttttac
    16801 cgcgtttaac cgacgcttca ttttgccgga acttcgcgac gcggtgcgta ccgtcagcgc
    16861 cgatattgtt tgcctgcagg aagtgatggg cgcgcacgaa gttcatccgc tgcatgtgga
    16921 aaactggccc gatacctcgc actacgagtt tctcgccgac actatgtgga gcgattttgc
    16981 ctacggtcgc aatgccgtat acccggaagg gcatcacggc aacgccgtac tgtcgcgtta
    17041 tcccattgaa cattatgaga atcgcgatgt ttcggtcgat ggtgcggaaa agcgcggcgt
    17101 gctctactgc cgcattgtgc cgccgatgac cggaaaagcg attcatgtga tgtgcgtaca
    17161 tctgggcctg cgtgaggcgc accgtcaggc gcagcttgcg atggggtgaa tgagctaccg
    17221 gacggcgaac cggtattggt ggcgggtgat ttcaatgact ggcggcaaaa agctaatcat
    17281 ccgttaaaag tgcaggccgg actggatgag atttttaccc gcgcccacgg acgcccggcg
    17341 cgcacgtttc cggtgcaatt tcctctacta cgactggaca ggatctacgt caaaaatgcc
    17401 agcgccagcg cgccaaccgc gttgccgctg cggacatggc gacacctttc tgatcatgcc
    17461 cctttaagtg cggagattca tttatgaaat gtagctggcg cgaaggcaat aagatccagt
    17521 tgctggaaaa cggcgagcaa tattatcccg cggtgtttaa ggcgattggc gaggcacaag
    17581 aacgcatcat tcttgaaacg tttatctggt ttgaggataa cgtcggcaaa cagctgcatg
    17641 cggcactact ggcagcagcg caacgcgggg ttaaagcgga agtcttgctg gatggctacg
    17701 gttcgccgga tctcagcgat gagtttgtca ataaactgac ggcagctggc gtagtgttcc
    17761 gctactacga tccccgccct cgcctttttg gtatgcgcac caatgtgttt cgccggatgc
    17821 atcgcaaaat tgtggtgatc gacgcgcgta tcgcctttat tggcgggctg aattactccg
    17881 ccgagcatat gtccagctac ggtccagagg ctaaacagga ttacgcggta cgccttgaag
    17941 ggccgattgt cgaagatatt ctccagtttg agctggaaaa cctgcctgga cagagcgcgg
    18001 cacggcgctg gtggcgacgt catcacaaag cggaagagaa ccgccagccg ggagaagcgc
    18061 aggtattgct ggtctggcgc gataacgaag aacatcgcga tgatattgaa ccccattatc
    18121 tgaaaatgct cactcaggcg cggcgggaag tgattatcgc caacgcctac ttcttccccg
    18181 gctatcgatt tttacacgcc ttgcgtaaag cggcacggcg cggggtgcgg atcaaactga
    18241 tcattcaggg cgaaccggat atgccgattg tcagagtcgg tgcgcgcttg ctgtataact
    18301 atctggttaa aggcggcgtt caggtttttg agtaccgccg ccgcccgctc cacggcaaag
    18361 tggcattgat ggacgatcac tgggcgacag tagggtccag taatctcgat ccgctcagtt
    18421 tgtcactgaa tctcgaagca aatgtcatca tccacgatcg tcattttaac cagacgctgc
    18481 gcgataatct gaacggcatt attgccgcag attgtcagca ggtggatgaa accatgctgc
    18541 ccaaacgcac ctggtggaac ctgaccaaaa gcgtgctggc gttccacttt ttacgccact
    18601 tcccggcgct ggttggctgg catccggcac acacaccacg tctgacgcag gttgatcccc
    18661 ccgcacaacc gacaatggaa acgcaggatc gggtagaaac tgaaaacacg ggggtaaaac
    18721 cctgatgagt aaatcacacc cgcgctagcg cttagcaaag aagatcctca cctggctgtt
    18781 ttttatcgcg gtgattgtgt tgctggtggt ctacgccaaa aaagtggact gggaagaggt
    18841 ctggaaggtc atccgcgact acaatcgcgt tgcgctgctt agtgcggtcg ggctggtggt
    18901 cgtcagctat ctgatttacg gctgctatga cctgctcgcc cgcttttact gcggtcacaa
    18961 actggcgaag cgccaggtga tgctggtgtc gtttatctgc tacgccttca acctgacgct
    19021 cagtacctgg gtcggcggca ttggtatgcg ctatcgtttg tactcccgac tagggttacc
    19081 gggcagcact attacgcgga ttttctcgct cagtattacc accaactggc tgggctatat
    19141 tttactggca gggattatct ttaccgcagg cgtggtggag ttgccggatc actggtatgt
    19201 cgctcaaact acgctgcgta ttctcggcat tggcttactg atgattatcg cggtttattt
    19261 gtggttttgc gctttcgcga agcaccgcca tatgaccatc aaagggcaaa agctggtgct
    19321 gccttcatgg aaattcgccc tcactcagat gctgatttcc agcgttaact ggatggtaat
    19381 gggggcgatt atctggctgt tacttggtca aagcgtgaac tatttctttg tactgggcgt
    19441 gttactggtt agtagtattg ctggcgtcat cgtgcatatt ccggcgggga tcggtgtgct
    19501 ggaagcggtg tttatcgcac tactggctgg ggagcatacc tcgaaaggga gcattatcgc
    19561 cgcgctgctc gcttatcgcg tcctgtatta ctttatcccg ctgctgctgg cgctgatctg
    19621 ttacctgcta ctggaaagcc aggcgaagaa gctacgggcg aaaaatgaag cggcgatgtg
    19681 aaatttagtt aagttcttta gtatctgcat tcacgactaa tgaaaaaaac gcgtatgcct
    19741 ttgccagaca agcgttatag ctatcgcggc aagtatccca aagaagccga taaaatagta
    19801 atagagtgtt aatgcacctg taaaagcgca acgaaccgga ctccacaact cttcactatg
    19861 gcattttcct gccagttcaa ggagcttatt tcgtccatgc gagataatgc ctggccataa
    19921 cacaatcact aagcaactta ttacccaaat gggccgttca ctcattaatg ccgcattcac
    19981 gatgattacc agggccaacc ctgtcaacgt catcataatg ataaaacggt gagaaccagg
    20041 atcaacatta aataataaac cggccagcat actgattaaa aacatcgcgc ccgcagtgct
    20101 gaacacaatg ata
//
LOCUS       DADLFA010000065        19875 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000065 DADLFA010000000
VERSION     DADLFA010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19875
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.065"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            54..830
                     /locus_tag="JQH65_001964"
     CDS             54..830
                     /locus_tag="JQH65_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HAY6139385.1"
                     /translation="MTSESCALDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTG
                     RAGPRPRTQALLRFLADHSRSKDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDM
                     GRRLCAEAVEALKAQCVANPDVQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVG
                     TPFFVRYGRVKIEDQIGEILGAKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEAD
                     RTCISNIHQGGTPPVEAAAVIVDLAKRMLEQKASGINMTR"
     gene            840..1499
                     /gene="eutL"
                     /locus_tag="JQH65_001965"
     CDS             840..1499
                     /gene="eutL"
                     /locus_tag="JQH65_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HAY6139386.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            1512..2012
                     /gene="eutK"
                     /locus_tag="JQH65_001966"
     CDS             1512..2012
                     /gene="eutK"
                     /locus_tag="JQH65_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HAY6139387.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAAGRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVREPD
                     APVIVAKSADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            2058..3110
                     /gene="eutR"
                     /locus_tag="JQH65_001967"
     CDS             2058..3110
                     /gene="eutR"
                     /locus_tag="JQH65_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HAY6139388.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFVTDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(3116..4015)
                     /gene="hemF"
                     /locus_tag="JQH65_001968"
     CDS             complement(3116..4015)
                     /gene="hemF"
                     /locus_tag="JQH65_001968"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HAY6139389.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(4019..4888)
                     /gene="amiA"
                     /locus_tag="JQH65_001969"
     CDS             complement(4019..4888)
                     /gene="amiA"
                     /locus_tag="JQH65_001969"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HAY6139390.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            5102..5527
                     /locus_tag="JQH65_001970"
     CDS             5102..5527
                     /locus_tag="JQH65_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase YpeA"
                     /protein_id="HAY6139391.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADMLSLGKRLIEDEEY"
     gene            5514..5963
                     /locus_tag="JQH65_001971"
     CDS             5514..5963
                     /locus_tag="JQH65_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HAY6139392.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFIIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            6170..6745
                     /locus_tag="JQH65_001972"
     CDS             6170..6745
                     /locus_tag="JQH65_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HAY6139393.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFRGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            6841..7740
                     /gene="yfeX"
                     /locus_tag="JQH65_001973"
     CDS             6841..7740
                     /gene="yfeX"
                     /locus_tag="JQH65_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HAY6139394.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(7798..9102)
                     /gene="pbp4b"
                     /locus_tag="JQH65_001974"
                     /pseudo
     CDS             complement(7798..9102)
                     /gene="pbp4b"
                     /locus_tag="JQH65_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
     gene            complement(9107..10531)
                     /gene="murP"
                     /locus_tag="JQH65_001975"
     CDS             complement(9107..10531)
                     /gene="murP"
                     /locus_tag="JQH65_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HAY6139395.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKSVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRSQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(10535..11431)
                     /gene="murQ"
                     /locus_tag="JQH65_001976"
     CDS             complement(10535..11431)
                     /gene="murQ"
                     /locus_tag="JQH65_001976"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAY6139396.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            11595..12452
                     /gene="murR"
                     /locus_tag="JQH65_001977"
     CDS             11595..12452
                     /gene="murR"
                     /locus_tag="JQH65_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HAY6139397.1"
                     /translation="MLYLTKISNAGSEFTENEQKIADFLQANVSELQSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNTTALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCVEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVASLKMIQR
                     SSELTQRLK"
     gene            12581..13372
                     /gene="ucpA"
                     /locus_tag="JQH65_001978"
     CDS             12581..13372
                     /gene="ucpA"
                     /locus_tag="JQH65_001978"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HAY6139398.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            13676..14692
                     /locus_tag="JQH65_001979"
     CDS             13676..14692
                     /locus_tag="JQH65_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY6139399.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKTDVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            14692..15525
                     /gene="cysT"
                     /locus_tag="JQH65_001980"
     CDS             14692..15525
                     /gene="cysT"
                     /locus_tag="JQH65_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HAY6139400.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            15525..16400
                     /gene="cysW"
                     /locus_tag="JQH65_001981"
     CDS             15525..16400
                     /gene="cysW"
                     /locus_tag="JQH65_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HAY6139401.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            16390..17487
                     /gene="cysA"
                     /locus_tag="JQH65_001982"
     CDS             16390..17487
                     /gene="cysA"
                     /locus_tag="JQH65_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HAY6139402.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            17621..18532
                     /gene="cysM"
                     /locus_tag="JQH65_001983"
     CDS             17621..18532
                     /gene="cysM"
                     /locus_tag="JQH65_001983"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HAY6139403.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPNNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(18535..18903)
                     /locus_tag="JQH65_001984"
     CDS             complement(18535..18903)
                     /locus_tag="JQH65_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HAY6139404.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLSQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            19008..19859
                     /gene="pdxK"
                     /locus_tag="JQH65_001985"
                     /gene_synonym="thiJ"
     CDS             19008..19859
                     /gene="pdxK"
                     /locus_tag="JQH65_001985"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HAY6139405.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
BASE COUNT         4575 a         5043 c         5607 g         4650 t
ORIGIN      
        1 cggcccccgc gctgtcagaa gcaaagtgca ccaccaccac ctgtgcggca ccggtgacct
       61 cggaaagctg cgcgctggat ttaggttctg cggaagcaaa agcgtggatt ggtgttgaaa
      121 acccgcatcg cgcagacgta ttaacagaac tgcgtcgcag caccgtggcc cgcgtttgta
      181 ccggtcgtgc cggtccgcgt ccgcgtaccc aggcgttgct gcgtttcctg gccgatcact
      241 ctcgttcgaa agataccgta ctgaaagaag tgccggaaga gtgggtgaaa gcgcagggct
      301 tgctggaagt acgctccgaa attagcgaca aaaatctcta cctgactcgc ccggatatgg
      361 gccgccgcct gtgtgcagaa gctgttgaag cgctgaaagc acagtgtgtt gctaatccgg
      421 acgtacaggt cgttatttct gatggcctgt caactgatgc catcaccgtg aactacgaag
      481 agatcctgcc gccgctgatg gcgggcctga aacaggccgg gctgaaagtc ggcacgccat
      541 tctttgttcg ttatggtcgc gtgaagattg aagatcagat cggtgagatc ctcggcgcga
      601 aagtggtgat cctgctggtg ggggaacgtc cggggttggg tcagtcagaa agcctctcct
      661 gttacgccgt ttactcgccg cgtatggcga ccaccgttga ggccgatcgc acctgcatct
      721 ctaacattca ccagggcggc acgccgccgg ttgaagccgc agccgtcatt gtggatttgg
      781 cgaaacgtat gctggagcag aaagcatccg gcatcaacat gacccgataa ggaggcatca
      841 tgccagcttt agatttgatt cgaccgtcgg taaccgccat gcgggtgatt gcctccgtta
      901 acgctgaatt tgcgcgtgaa ctaaaattgc cgccgcatat tcgtagcctc gggctgattt
      961 ctgctgattc cgatgacgtc acctacattg cggctgacga agcgaccaag caggcgatgg
     1021 tcgaagtggt atatggccgc tcgctgtatg cgggagcagc acacggaccg tcaccgactg
     1081 ccggtgaagt gctgattatg cttggtgggc caaacccggc ggaagtgcgt gctggtctgg
     1141 atgcgatggt tgcgcatatt gaaaatggcg ctgctttcca gtgggctaat gacgcagaaa
     1201 acaccgcatt cctggcacat gtagtttcgc gtaccggttc ttatctctca tcaaccgccg
     1261 ggatcacgct tggcgatccg atggcgtatc tggtggcacc gccgctggaa gcgacctacg
     1321 gcattgatgc agcgttgaaa tctgccgacg tacagctggt gacctatgtc ccgccaccgt
     1381 ctgaaaccaa ctactcggca gcatttttaa ccggtagcca ggccgcgtgt aaagcagcct
     1441 gtaacgcctt taccgatgca gtgctggaaa tcgcgcgtaa tccaatccag cgtgcgtaac
     1501 ggaggttgcc gatgatcaat gcactgggat tgctggaagt ggacggcatg gtcgccgcga
     1561 tagatgcagc ggatgccatg ctcaaagcag ctaacgttcg tctgctcagt cacgaagtgc
     1621 ttgaccctgg tcgcttaacg ctggtggtgg aaggcgatct ggcggcgggt cgtgcggcgc
     1681 tggacgcagg ttgtgctgcc gcgatgcgta ccgggcgtgt catcagccgc aaggagatcg
     1741 gtcggccaga cgatgacacc cagtggctgg ttactggctt taaccgccag ccgaagcaac
     1801 ccgtaaggga acccgacgcg ccagttatcg tcgcgaaatc tgctgacgag ttgttggcgc
     1861 tgttaacatc agtacgtcag ggaatgacgg caggagaagt ggctgcccac tttggctggc
     1921 cgctggaaaa agccagaaat gcgctcgaac agctcttttc tgccgggacg ttacgtaaac
     1981 gcagtagtcg ttatcgtctc aagccccatt aacctgtcgg aggtgctggg tgtcctacaa
     2041 cacccggcat taacatcatg aaaaagaccc gtacagccaa tttgcaccat ctttatcatg
     2101 aacccttacc cgaaaacctg aagctcacgc cgaaggtcga agtggataat gttcatcaac
     2161 gacagacaac ggatgtctat gaacatgctt taacgattac cgcctggcag cagatttacg
     2221 atcagctgca tccgggcaag tttcatggtg aatttacgga aattctactc gatgatattc
     2281 aggtttttcg tgaatacacc ggtctggcgc tgcgtcagtc gtgcctagtc tggccgaact
     2341 cgttctggtt tggcattccg gcgacgcgcg gtgagcaggg atttatcggt tcgcaatgtc
     2401 tgggaagcgc ggaaatcgcc acccgccctg gtggcactga atttgaactg agcacgccgg
     2461 atgattacac gatcctgggc gtggtgcttt ctgaagatgt catcacccgg caggctaact
     2521 ttttgcataa cccggatcgg gtattacata tgctgcgtag ccagtcggcg ctggaagtga
     2581 aagagcagca taaagccgcg ctgtggggct ttgtccaaca ggcgctggcg acgttttgcg
     2641 agaacccgga aaatctccat cagcctgcag tgcgaaaagt gctgggggat aatttgctaa
     2701 tggcgatggg ggcgatgctg gaagacgcgc aaccaatggt gacggcggaa agcatcagtc
     2761 atcagagtta ccgtagatta ctttcccgcg cccgtgaata tgtgctggaa aatatgtctg
     2821 agccggtgac ggtgctggac ttgtgtaatc aactgcatgt cagtcgccgc acgctacaaa
     2881 acgcgtttca cgctatttta ggcattggcc caaacgcgtg gctgaaacgc attcgcctga
     2941 acgccgtacg ccgcgaactg ataagcccgt ggtcgcaaag cacaacggta aaagacgccg
     3001 ccatgcagtg gggattctgg catctggggc aatttgtcac ggattaccag cagctgtttg
     3061 ccgagaagcc gtcgttgacg ttgcatcagc ggatgcggga atgggggtga gggagttaca
     3121 cccaatccct gaccttaata aactcactta acgccgcttc tgggctgcca tcttttggct
     3181 gataatcata ctcccagcgt accagtggcg gcattgacat caggatcgac tcggtgcgcc
     3241 cgccagtttg caggccaaac agagtgccgc gatcccagac cagattgaac tcgacataac
     3301 gaccgcgacg gtagagctga aaattgcgct cgcgctcgcc gtaggccatt gctttacgtc
     3361 gctctacaat tggtaaataa gcgtcggtgt agcctttgcc taccgcctgc ataaaggcaa
     3421 aacagtggtc gaaatctggc gtgttcagat catcaaagaa cagcccgcca ataccgcgct
     3481 gttcgttgcg atgtttgagg tagaagtaat cgtcgcacca ctttttgtaa cggggataaa
     3541 cgtcttcacc aaatggcagg cacaggtcac gggcggtgcg gtgccagtga atggcgtctt
     3601 cttcaaaacc atagaaaggg gttaaatcga agccgccgcc aaaccaccag acgggctctg
     3661 cacccggttt ttcggcaata aaaaaacgca cattcgcgtg gctggtggga acatacgggt
     3721 tatgcggatg cactaccagt gaaacgccca tcgcctcgaa actgcgcccg gcaagttccg
     3781 ggcgatgagc ggtggcggaa gcaggcatcg cctcgccgtg gacgtgagaa aagttgacgc
     3841 ctgcctgttc gaaaacacca ccattacgca acacccgact acgcccgccg ccgccagctt
     3901 cgcgctgcca actatcttcg acaaattctg cgccatcgac ggcggtcagc tgctgacaaa
     3961 tcgtatcctg aaggttgagc agaaactgtt taacctggtg tgcgtcgggt ttcataactt
     4021 atcgcttttt cgaatgtgct ttctggttgt cgaaccagtg gaaataactg atcacgcctt
     4081 cagcaatcgc tgtggcgatt ttctgacgaa acgccgccgt gcctaacagc cgctcttctt
     4141 ccgggttggt gataaacgag gtttccacca gcaccgaagg aaccgacggc gatttcaaca
     4201 ccacaaacgc ggcttgttcg gtgttgcggc tgtgcagttt atgcaccggc ttaatctttt
     4261 tcagaatatg cgagccaagc gtcaggctgt ttttaatagt atcggtttgc accagatcaa
     4321 acagcacttg ctgcaatagg tgatccttgt cagtcgcctt tttaccggca acttcatcgg
     4381 cgcggttttc gcgttcagac aggtatttcg ccattgcgct acttgcccca cggttagaga
     4441 gggcaaatac cgaagcaccg gcagctttcg ggttggtaaa gccatcggca tgaattgaca
     4501 taaacagatc tgcgccatgt ttatgggcga tttcaacgcg atcgtaaagt gggataaacg
     4561 tatcgccaga acgcgttaaa cgcgcatcaa tcccatgatt acgcaaaatg gaacggacgt
     4621 ttttagcaat cgccagcacc acatgttttt ctttcgaacc gttgcgtccg atcgctccgg
     4681 tatcaattcc gccgtggcct ggatcgagaa caacgacacg tttgccgcca gattttttgg
     4741 ctttcggctt gctgtgtccg ttgctggttt ttaaaggttc gtctttggcg atggcttgcg
     4801 acattcctga caacgtcagg gcagccaaac cggctttcag cacctggcgg cgcgaagtga
     4861 gtgtttttag tggtttaaaa gtgctcatac ggcctgagtt gtaataataa agttccagat
     4921 gttatatcgt atcgcgtaat ccgttacgac ggtttgatgt tgagaattgt tttacttttc
     4981 atttcaatag ttgacaactc cccattatgc cactttttca accgattttc gtcagatatt
     5041 ggctaattct accgttcacg ccataatcag ccttttaacc cgcaaaaagg taacgaatac
     5101 catggagata cgcgtatttc gccaggaaga tttcgaagag gtcatcaccc tttgggagcg
     5161 ttgcgacttg ctgcgtccgt ggaacgatcc ggaaatggac atcgagcgta agatgaacca
     5221 tgacgtcagt ttgtttttgg tcgctgaggt gaacggtgaa gtggtcggaa cggtgatggg
     5281 cggttatgac gggcatcgcg ggtctgctta ttatcttggc gtgcatccag agtttcgtgg
     5341 gcgtgggatt gccaatgcgt tgcttaatcg gctggagaaa aagctgattg ctcgtggctg
     5401 cccgaaaatt cagatcaatg tgccggaaga taacgacatg gtgctcggaa tgtatgaacg
     5461 cctgggatat gaacacgccg acatgctgag tctgggtaag cgtttgattg aagatgaaga
     5521 gtactgagtt ccatcctgtc cattatgatg cgcacggacg cctgcgttta cccttgctct
     5581 tctggcttgt gctattgctt caggcgcgaa cctgggtgct gtttatcatt gccggtgcgt
     5641 cgcgcgagca gggtactgcg ctgctaaatc tgttttatcc cgatcacgat aatttctggc
     5701 tggggttgat tcccggtatt ccggcggtgc tggcgttttt gttgagcggt cggcgggctt
     5761 cgtttcctcg cacctggcat gtgctctatt ttctgttgct gttggcgcag gtggttttac
     5821 tttgctggca accgtggctg tggctgaacg gtgaatccgt tagcggtatc ggtctggcgc
     5881 tggtagtggc ggatattgtg gcgttaatct ggctgctgac caatcgacgt ttacgcgctt
     5941 gttttaatga agaaaaagaa taacgcagag gtgcgctttg tttatgccgg atgcggcgtg
     6001 aacgccttat ccggcctaca aaacatcgaa aattcaatat attgcaggag aaagtaggcc
     6061 tgataagcgt agcgcatcag gcaattttgt gtttgtcatc aacctttaac ggcacttttt
     6121 ggtgaatttg cactccaagc aacgttattg aataaccaaa ggaagtgaca tgaaatcgct
     6181 gcgtttaatg ttatgcgcta tgccgttgat gctgaccggc tgttccacga tgtcgtcagt
     6241 taactggtcg gctgctaacc cgtggaactg gtttggatcg tccaccaaag tgagcgagca
     6301 gggtgtgggt gaattaacgg cgtccacacc actgcaagaa caagccattg ctgatgcgct
     6361 tgatggcgat tatcgcctgc gcagcggaat gaaaaccgcg aacggcaacg tggtgcgctt
     6421 ttttgaagtg atgaaaggcg acaacgtggc gatggtgatt aacggcgatc aaggcacgat
     6481 cagtcgcatt gacgtgctgg atagcgatat tcctgctgac accggtgtta aaatcggtac
     6541 accgtttagc gacctttaca gcaaagcatt tggcaattgc caaaaagctg atggtgatga
     6601 taatcgtgcc gtcgaatgta aagccgaagg cagtcaacat attagctacc agttcagagg
     6661 ggaatggcgt ggtcctgaag ggttaatgcc ttcggacgat accctgaaaa actggaaagt
     6721 cagtaaaatt atctggcggc gttaatttgc gtctgaacaa accgccgccg cgaaaaaaca
     6781 cgtaaaatag cgctcaacaa tgccacggat tgcgtggcat tcttattttc aggaggaaca
     6841 atgtctcagg ttcagagtgg cattttgcca gaacattgcc gcgcggcgat ttggatcgaa
     6901 gccaacgtga aaggggaagt tgacgccctg cgtgcggcca gtaaaacatt tgccgacaaa
     6961 ctggcaactt ttgaagcgaa attcccggac gcgcatcttg gtgcggtggt tgcctttggt
     7021 aacaacacct ggcgcgctct gagcggcggc gttggggcag aagagctgaa agattttccg
     7081 ggctacggta aaggccttgc gccgacgacc cagttcgatg tgttgatcca cattctttct
     7141 ctgcgtcacg acgtaaactt ctctgtcgct caggcggcga tggaagcctt tggtgactgc
     7201 attgaagtga aagaagagat ccacggcttc cgttgggtgg aagagcgtga cctgagcggc
     7261 tttgttgacg gtacagaaaa cccggcgggt gaagagacgc gccgcgaagt ggcggttatc
     7321 aaagacggcg tggatgccgg cggcagctat gtgtttgtgc agcgttggga gcacaacctg
     7381 aagcagctca accggatgag cgttcacgat caggagatga tgatcgggcg caccaaagag
     7441 gccaacgaag agattgacgg cgacgaacgt ccggaaacct ctcacctcac ccgcgttgat
     7501 ctgaaagaag atggcaaagg gctgaagatt gttcgccaga gcctgccgta cggcaccgcc
     7561 agtggcactc acggtctgta cttctgcgcc tactgcgcgc gtctgcataa cattgagcag
     7621 caactgctga gcatgtttgg cgataccgat ggtaagcgtg atgcgatgtt gcgcttcacc
     7681 aaaccggtaa ccggcggcta ttacttcgcg ccgtcgctgg acaagctgat ggcgctgtaa
     7741 gcgtttctga ttctatgatt aaaggccagc cagtaacaaa actgactggc ctttttatta
     7801 cttttgcttc aacgccgcat acacctgatc aactacccaa ccataagttg caatcggtaa
     7861 ctgaccgctt tcgaacatat tgggattttt ttgtggatcg gcaactggcg aatggggctt
     7921 gttgcttaac atcacaatcg ccatatgatt gaccggatcg ataacggtta ctgtcccggt
     7981 ccagccagtg tgaccgtagg tttgcgggct tgccagcgtg ccaaacgtcg gcgtcatggt
     8041 ggcattacca ttcacgcgcc agccgaggcc aaaagtggca tcttccttag agctggtggt
     8101 gaacatcttc accgtttccg cactgaacag ctgcacatcg ccatagcccc cgccgttcag
     8161 catcgtttgc attaacaccg caatatcgcc ggtattggaa aacaaacctg cgtgcccgga
     8221 aacgccgccc atcgaataaa aggctttttc atcgtgcacc tgaccccaga gagtggaggt
     8281 gcggatattc gggaaatgga tcacgccatc gcgggtattg ccgttcaatt ccgtggcggc
     8341 aatttgttgc ggtttaaagc ctttcagcag cgggttaaat accgtatgtg tcaggccgag
     8401 cgggcgataa atcgactctt caacatagcg gtcaagcggc tgaccggtaa ccgactcgac
     8461 gataaatcca agcagcatat aatcgacatc gctgtagata tgtttgctgc cgggctgata
     8521 ttccagcggc gtgcgcttga tcatctccag cgtctggcct ttatcctagg aatataacgc
     8581 gcccgcgacc gctttattcg ggtattgcgg atctgccggg aaaccgccgc tgtgatgcag
     8641 caggtcagaa atccgcaggg tgtttttgcc tttaatggtg tcatttggac tatcggcaaa
     8701 ccccggaata tatttcgcaa tccgatcgtc aggatgcagt ttgccttcgc tcatcagctt
     8761 ttgcagggcg aagttcgtgg cgtacatttt ggtgtttgag gccaggtcat acagcgtccc
     8821 ggtggtggct ttgacaggct gttccatcaa cacgctgcca tcgtactttt tcgccgctcc
     8881 ccaggctttg cgatacacaa tctgattatc tttaatgatc agcaggttta cgccgggata
     8941 accggcatca acttgctggc taatccagcg atccatctgg ttaagccgtt tgacgttaaa
     9001 ccctgctttc tctggcgagc tttctgtcag aacagggtat tttgcggcgc tgacgctaca
     9061 actaacagcc agcagagaaa gatagagcat tgtccgtttc atgatatcag tccagattga
     9121 cgttacggca gccaaagagc gtggtgaaaa taaacccgca aacccatgcc accagaatcc
     9181 cgccagcata aaccgccatg gccggaagga tgccttgtgc ggaagtcatc agcggcagcg
     9241 ctaccagacc agacggccca aaggcgctgt ttaagcccat cggcagaccc caccaggcta
     9301 tcaggccgat aaacaaaccg cccgccgcac cgcctaaaca ggcggtgaca aacggcttca
     9361 tgcggggcag ggtgacaccg taaatcagcg gttcaccaac gcccagcagg ccgggaataa
     9421 tcgccccgcg tacctgactg cgtagcgcac tgtgcggttg cgaccgccag tagagtgcca
     9481 gcgccgcgcc cacctggccc gcgcctgcca ttgaaaggat gggaaataag ctgttgaatc
     9541 cctggctgtc cattaacgcg aggtaaacag gaataaagcc ctgatgcacg ccaaatacca
     9601 cggcgatcag gaacagcccg gctaaaaccg cacaaccgaa cggattactg ttcaggtgca
     9661 taaacagcca tgacatacct tcgaataacc agccacccag cggcataatg atcaggtacg
     9721 caagcgtagc ggtgatcagc agggtgatta acgaggtcag cagcatgtcg agatcgtccg
     9781 gcataaagcg gcgcaccatg ccttcaatgc gggcgcaggc ccacgcggca atcagcacgc
     9841 cgataatatt gccgcgcgga tcgatgggca gaccaaagaa atcgtgaaaa ccggcgtagt
     9901 aaccggtggt tgcagcgggg ttataaccga gcaaaaagag tgcggcaata attgcgccat
     9961 tgacgcccgt gccgccaaat gcctgggcgg cgttatagcc caccagaatc accaggaagg
    10021 tgaacaaacc tttgctgaac accttcataa aattcagcgc gtcgggtagc gtgccctgag
    10081 catctgccgg aacgtgcatc accgtggcaa ttaacgtcgc tatccccagc agcagaccgg
    10141 cggcaataaa accggggatc agcggcgtga agatggtggc gaatttggcg agaaattgtt
    10201 gcacgccgga ggtttgtttg gcttttaact gacgtttgtt ctgggcggcg atttctgcgg
    10261 catcctgtac cggtgcctcg cccagcagct cgctcatcgc ttttgcggca cgatgcgctt
    10321 taccaggtcc aaaaacgacc tgcacctggt cgctggtcaa aatgacgctc ttcacgcctt
    10381 ccagcgtttt gatattggga tcaaccagtg aactgtcatg tacacccaga cgcaggcgcg
    10441 tcatacagtt accacaactg gcgatatttc ccggtccgcc gacacgggta agaatggtgt
    10501 tcagaagttc actgctgatc tctttggcca tgggttattc cttgtctaaa acctgacgaa
    10561 taaagccgcc gtgttgatcc aggcgttttt tagcttctgc ggcatcgaga tttttcagca
    10621 ccatcacaat ggccgtttta cagttgcgct cgcaggcaat taacgccgct tccgcttgtt
    10681 cggcattaca tccggtggcg tttttaacga tattgacctg gcgcacatgc agtttttcgt
    10741 tggtggcgac cacatcgacc ataaggttgc cgaacacttt gccggattta atcatcagcc
    10801 cggtggaaag catattgagc accagtttct gcgctgtacc tgctttcatc cgcgaagaac
    10861 cggtaacaac ttcggcacct acaatcggtg taatggcaaa ctcagcggtg gttgaaacgg
    10921 cgctccccgg attacaggaa attcccactg tgcggcagcc gagctggcgt gcgtattcca
    10981 gtccggcaat cacatacggc gtgcgaccgc tggcagcaat gccaaccacc acatcctgtg
    11041 ccgttaaatt aatatttttc agatcattaa caccgccttc ccggctatct tccgcgcctt
    11101 ccaccgcgtg ctgaatggca tattcgccgc cagcaatcaa accaaccacc agacccggtt
    11161 tcacgccgta ggtgggcgga cattcgctgg catccagaat ccccagacga ccggatgttc
    11221 ccgcaccgag gtaaatcaga cgcccgccgc cgcttacctg ggcgtggata acatcgatcg
    11281 ccgcggcgat atccggcagt acgcgctcaa cggcaagcgg tacggtttta tcttcatcgt
    11341 tgataatccg gcacatttcc agcgttgata cgcggtcaat ttcagccgag gcggcgttcg
    11401 agccttcagt aatcatcttt tcaagttgca tcggtggtga ccttactatt tacgatcaaa
    11461 taattgcaga tgtatggaat catatatcct gaattgacag acttaaagga atcattgagt
    11521 ctacactcgc agagtgtgaa gagcgtcata agaacttaag cggaatttat gctgccagcc
    11581 caggagagtg gaagatgttg tacctgacga aaataagcaa tgcggggagt gaattcaccg
    11641 aaaacgagca aaaaatcgct gactttctgc aggccaacgt gagtgagctg caatccgttt
    11701 cttcacggca gatggcgaaa cagctcggca ttagccagtc gagcatagtg aaatttgccc
    11761 aaaaactcgg cgcgcaagga tttactgaat tgcgtatggc gttaataggc gaatacagtg
    11821 ctagccggga gaaaacgaat accacggcgc tgcacctgca cagttcgatt accagtgatg
    11881 attcgctgga agtgattgcc cgcaaattga atcgcgaaaa agagctggcg ctggagcaaa
    11941 cctgtgcgtt gttcgactac gcgcggctgc aaaaaatcat tgaggtcatc agtaaagcac
    12001 cgtttatcca gataacgggc ctgggcggat cggcgctggt ggggcgcgat ctgtcgttca
    12061 aactgatgaa aattggctat cgcgtggcct gcgaagccga cacgcacgta caagccaccg
    12121 tttcccaggc gttgaaaaaa ggcgatgtac aaatcgctat ctcttacagc ggtagtaaaa
    12181 aagagattgt cttgtgcgta gaagcggcac gaaagcaggg ggcaacggtg attgccatta
    12241 cttctctggc tgactctccg ctgcggcggc tggcgcattt cacgctcgat accgtttccg
    12301 gtgaaaccga atggcgtagc tcgtcgatgt ctacccgcac ggcgcaaaat tctgttaccg
    12361 atttactgtt tgttggtctt gtgcaactca atgatgtggc gtcattgaaa atgattcagc
    12421 gcagcagtga gctaacacaa cgcctgaaat gatgcataaa gcagcgactg gattgagatt
    12481 ttcctgaatt agtgagctga tccgcagcaa tattttgttt atcctgtatt ttcagaggga
    12541 atggagtgta acgctctgta ttaacaagga gagcattaaa atgggtaaac tcacgggcaa
    12601 gacagcactg attacgggcg cattgcaggg aattggcgaa ggaattgcca gaacttttgc
    12661 acgtcatggc gcgaacctaa tcttgctgga tatctcccct gagatcgaaa agctggcgga
    12721 cgaactgtgt ggtcgtggtc atcgctgtac ggcggttgtc gccgatgtgc gtgacccggc
    12781 gtcggtagcc gcagctatca aacgcgcgaa ggaaaaagaa gggcgcattg atatcctggt
    12841 gaataacgca ggcgtttgtc gtctgggcag tttcctcgat atgagcgatg acgatcgcga
    12901 tttccatatt gatatcaata ttaaaggcgt atggaacgtc acgaaggcgg tgctgccgga
    12961 gatgattgcg cgcaaagatg gtcgcattgt gatgatgtct tcagtcactg gtgatatggt
    13021 ggccgatcct ggcgaaacgg cgtacgcctt aacgaaagcg gcgattgttg gcctgacaaa
    13081 atcgctggcg gtggagtacg cgcagtctgg tattcgcgtt aacgccattt gcccgggata
    13141 cgtgcgcaca ccaatggcgg aaagcattgc ccgccagtcg aacccggaag atccagagtc
    13201 ggtgctgact gaaatggcga aagcaatccc gatgcgtcgc ctcgccgatc cgctggaagt
    13261 cggcgaactg gcggccttcc tcgcatcgga tgaatccagc tatttaaccg gtacacagaa
    13321 tgtgattgat ggcggcagca cactgccgga gacggttagc gtcggtatct gattcacctc
    13381 tgtttcctcc ctgcatgtgt ggggaggatt tcgtcttgaa ctaagttcac caggctattt
    13441 tatttgtcat tttggccccg ggcagtgctc gaaatactca cgtactatgt gtacgctccg
    13501 gtttctccgc gctgtccgtg tccaaactgc ctgcaacaat tacgcctgtt gaaccaagtt
    13561 cttattccct ttttaacttc caaatcacca aacggtatat aaaaccgtta ctcctttcac
    13621 gtccgttata aatatgatgg ctattagaaa gtcattaaat ttataagggt gcgcaatggc
    13681 cgttaactta ctgaaaaaga actcactcgc gctggtcgct tctctgctgc tggcgggcca
    13741 tgtacaggca acggaactgc tgaacagttc ttatgacgtc tcccgcgagc tgtttgccgc
    13801 cctgaatccg ccgtttgaac agcaatgggc aaaagataac ggcggcgaca aactgacgat
    13861 aaaacaatct catgccgggt catcaaaaca ggcgctggca attttgcagg gcttaaaaac
    13921 cgacgttgtc acttataacc aggtgaccga cgtacaaatt ctgcacgaca aaggcaagct
    13981 gatcccggct gactggcagt cgcgcctgcc gaataatagc tcgccgttct actccaccat
    14041 gggcttcctg gtgcgtaagg gcaacccgaa gaatatccac gactggaacg acctggtgcg
    14101 ctccgacgtg aagctgattt tcccgaaccc gaaaacgtcg ggtaacgcgc gttataccta
    14161 tctggcggca tggggcgcag cggataaagc tgacggtggc gacaaagcca aaaccgaaca
    14221 gtttatgacc cagttcctga aaaacgttga agtgttcgat actggcggtc gtggcgcgac
    14281 caccactttt gccgagcgcg gcctgggcga tgtactgatc agcttcgagt cggaagtgaa
    14341 caacatccgt aaacagtatg aagcgcaagg ctttgaagtg gtgattccga aaaccaacat
    14401 tctggcggaa ttcccggtgg cgtgggtcga taaaaacgtg caggccaacg gtacggaaaa
    14461 agcagcaaaa gcctacctga actggctcta cagcccgcag gcgcagacca tcatcaccga
    14521 ctattactac cgcgtaaata acccggaagt catggacaaa ctgaaagata aattcccgca
    14581 gaccgagctg ttccgcgtgg aagacaaatt tggctcctgg ccggaagtga tgaaaaccca
    14641 cttcaccagc ggcggcgagt tagacaagct gttagcggcg gggcgtaact gatgtttgca
    14701 gtttcatcaa gacgcgtgct gccgggcttt accttaagcc tcggcaccag tctgctgttt
    14761 gtgtgcctga ttttgctgct gccgctctcc gcgctggtga tgcagctggc tcagatgagc
    14821 tgggcgcagt actgggaggt gatcaccaac ccacaagtgg tcgcggccta caaagtgacg
    14881 ctgctgtcgg cgtttgtggc atcgattttt aacggcgttt tcggtctgct gatggcgtgg
    14941 attttaacac gttatcgctt tccgggccgc acgctgcttg atgcgctgat ggatttaccc
    15001 tttgcgctgc caacggcagt cgccggttta acgctggcct cgctcttttc cgtaaacggt
    15061 ttttacggtg aatggctggc gaagtttgat atcaaagtca cctatacctg gcttggtatt
    15121 gcggtggcga tggcctttac cagcattccg tttgtggtgc gtaccgtgca gccggtgctg
    15181 gaagagttag gcccagaata tgaagaagcg gcggaaacgc ttggtgcaac gcgctggcag
    15241 agtttctgca aagtggtgct gccggaactt tctccagcgc tggtggcggg cgtggcgttg
    15301 tcgtttaccc gtagtcttgg tgagtttggc gcggtgatat ttatcgccgg gaacatcgct
    15361 tggaagacgg aagtgacgtc gctgatgatt tttgtgcgct tgcaggagtt tgattacccg
    15421 gcggcgagcg cgattgcttc ggtgatcctc gcggcatccc tgctgctgct gttctcaatt
    15481 aacactctgc aaagtcgctt tggtcggcgt gtggtaggtc attaatggcg gaagttaccc
    15541 aattgaagcg ttatgacgcg cgcccgatta actggggcaa atggtttctg attggcatcg
    15601 ggatgctggt atcggcgttc atcctgctgg tgccgatgat ttatatcttc gtacaggcgt
    15661 tcagcaaagg gctgatgccg gttttacaga atctggccga tccggacatg ctgcacgcca
    15721 tttggctgac ggtgatgatt gcgctgattg ccgtaccggt gaacctggtg ttcggcattc
    15781 tgctggcgtg gctggtaacg cgctttaact tccccggacg ccagttactg ctgacgctac
    15841 tggacattcc gtttgccgta tcgccggtag ttgccggtct ggtgtatctg ctgttctacg
    15901 gctctaacgg tccgctgggc ggctggctgg atgaacataa tttgcagatt atgttctcct
    15961 ggccgggaat ggtgctggtc accatctttg tgacctgtcc ttttgtggtg cgcgagctgg
    16021 tgccggtgat gttaagccag ggcagccagg aagacgaagc ggcgattttg cttggcgcgt
    16081 ctggctggca gatgttccgt cgcgtcacat tgccgaacat tcgctgggcg ctgctgtatg
    16141 gtgtggtgct aaccaacgcc cgcgcgattg gcgagtttgg tgcggtgtcg gtggtttccg
    16201 gctcgattcg cggcgaaaca ctttcgctgc cgttacagat tgaattgctg gagcaggact
    16261 acaacaccgt cggctccttt accgccgccg cgctgttgac gctgatggcg attatcaccc
    16321 tgtttttaaa gagtatgttg cagtggcgtc tggagaatca ggaaaaacgc gcgcagcagg
    16381 aggagcatca tgagcattga gattgccaat attaagaagt cgtttggtcg cacccaggtg
    16441 ctgaacgata tctcactgga tattccttct ggtcagatgg tcgcgctgct ggggccgtcc
    16501 ggttccggaa aaaccacgct gctgcgcatt attgccgggc tggagcatca aaccagcggg
    16561 catattcgct tccacggtac cgacgtgagc cgcctgcacg cacgcgatcg taaagtcggt
    16621 ttcgtgttcc agcattacgc gctgttccgt catatgacgg tgttcgacaa tatcgctttt
    16681 ggcctgacgg tgctgccacg tcgcgagcgc ccgaatgccg cagccatcaa agcgaaagtg
    16741 acaaaattgc tggagatggt ccagcttgcg catctggcgg atcgttatcc ggcacagctt
    16801 tccggcggcc agaaacagcg cgtggcgctg gcgcgcgcgc tggctgttga accgcaaatt
    16861 ctcctgcttg atgaaccgtt tggcgcgctg gatgcgcagg tgcgtaaaga gctgcgtcgc
    16921 tggctgcgtc aactgcatga agagctgaaa ttcaccagcg tgttcgtgac ccacgaccag
    16981 gaagaagcga ccgaagtagc tgatcgtgta gtggtgatga gccagggcaa tatcgagcag
    17041 gctgatgcgc cggaccaggt atggcgcgaa ccagcgaccc gatttgtgct cgaatttatg
    17101 ggcgaagtga accgcctgca gggaaccatt cgcggcgggc agttccatgt tggcgcacat
    17161 cgctggccgc tggggtatac gcctgcgtat caggggccgg tggatctctt cctgcgcccg
    17221 tgggaagtgg atatcagccg ccgtaccagt ctcgattcgc cgctgccggt acaggtactg
    17281 gaagccagcc cgaaaggtca ctacacccaa ttagtggtgc agccgctggg gtggtacaac
    17341 gaaccgctga cggtcgtgat gcatggcgac gatgccccgc agcgtggcga gcgtttattc
    17401 gttggtctgc aacatgcgcg gctgtataac ggcgacgagc gtatcgaaac ccgagatgag
    17461 gaacttgctc tcgcacaaag cgcctgatag gttgagtgaa tgttaaacgc ccggaggcgc
    17521 ttcccgcgat ccgggctttt taatggcaag gtttgtaacc tgtagacctg ataagacgcg
    17581 caagcgtcgc atcaggcaac accacgtatg gatagagatc gtgagtacat tagaacaaac
    17641 aataggcaat acgcctctgg taaagttgca gcgaatgggg ccgaataacg gcagtgaagt
    17701 ctggttgaaa ctggaaggca ataacccggc aggttcggtg aaagatcgtg cggcactttc
    17761 gatgatcgtc gaggcggaaa agcgcgggga aattaaaccg ggtgatgtgt taatcgaagc
    17821 caccagtggt aacaccggca ttgcgctggc aatgattgcc gcgcttaaag gctatcgcat
    17881 gaaattgctg atgcccgaca acatgagcca ggaacgccgt gcggcgatgc gtgcttatgg
    17941 tgcggaactg attcttgtca ccaaagagca gggcatggaa ggtgcgcgcg atctggcgct
    18001 ggagatggcg aatcgtggcg aaggaaagct gctcgatcag ttcaataatc ccgataaccc
    18061 ttacgcccat tacaccacta ccgggccgga aatctggcag caaaccggcg ggcgcattac
    18121 ccattttgtc tccagcatgg gaacaaccgg cactatcacc ggcgtttcac gctttatgcg
    18181 cgaacaatcc aaaccggtga ccattgtcgg cctgcagccg gaagaaggca gcagtattcc
    18241 cggcattcgc cgctggcctg cggaatatct gccggggatt ttcaacgctt ctctggtgga
    18301 tgaggtgctg gatattcatc agcgcgatgc ggaaaacacc atgcgcgaac tggcggtgcg
    18361 ggaaggaata ttctgtggcg tcagctcagg cggcgcggtt gctggagcac tgcgggtggc
    18421 aaaagctaac cctggtgcgg tggtggtagc gatcatctgc gatcgtggcg atcgctacct
    18481 ctccaccgga gtgtttgggg aagagcattt tagccagggg gcggggattt aagattattg
    18541 cgcaggcacc gttttatccg tctgcgcaat tagcgcatgt aaaaaaggct gtgcgttctc
    18601 atcgctctta ccggggatct tcacaatata cggcttcccg gtaatcgatg acgacgaagc
    18661 aaccttatca ataaactgct cggcagtgtc aatgcggtta cgggtattgc cgagcttcag
    18721 acgcagatga gaaaccgctt catcgcaggt atgttcatca ccgttgcgca caaatatcaa
    18781 atcctttttc tgtgataatc cctccagcat ggcgttgatg cgggcttctt catgggcagt
    18841 taacttcgcg taagcgggga gtgtcattag tagtgtaatg accagacaaa tgattttctt
    18901 cattgaatta ccttatcctt gttttgtaat agccgatttg actgccagaa tcaggtaaag
    18961 tttcggttgg aatacgatga ggatcatcgg caaggagaaa aaattaaatg agtagtttgt
    19021 tgttgtttaa cgataagagc agggcgctgc aggcggatat cgtcgccgtg cagtcgcagg
    19081 tggtttacgg cagcgtgggc aacagcattg ccgtgcctgc tatcaaacag aacggtctga
    19141 atgtttttgc cgtgccgacg gtattgctga gcaatacgcc gcattatgac actttctacg
    19201 gtggtgcgat tccggatgaa tggtttagcg gttatttgcg tgcgcttcag gagcgtgatg
    19261 cgctgcgcca gctgcgcgct gtaaccacgg gctatatggg aacggcatcg caaatcaaaa
    19321 tccttgccga gtggctgact gcgctacgca aagaccatcc tgacctattg atcatggtcg
    19381 atccggtgat tggcgatatt gatagcggaa tttatgtcaa acctgacctt cccgaggcgt
    19441 atcgacaata tttactgccg ctggcgcagg gaattacccc caatatcttt gagctggaaa
    19501 tcctgaccgg taaaaattgc cgcgatctcg acagtgccat tgctgccgca aaaagtctgc
    19561 tttcagacac attaaaatgg gtggtgatta ccagcgcctc cggtaatgaa gaaaatcagg
    19621 agatgcaggt ggtggtggtc agtgccgaca gcgtgaatgt catttcccat tcacgggtaa
    19681 aaaccgacct gaaagggact ggcgacctgt tttgtgctca gcttatcagt ggtttgctga
    19741 aagggaaggc gttaaacgat gcggtgcacc gtgcggggtt gcgcgtactg gaagtgatgc
    19801 gctacaccca gcagcatgag agcgatgaat tgattttgcc gccgctggcg gaagcataaa
    19861 aaatggcgcc gatgg
//
LOCUS       DADLFA010000066        19822 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000066 DADLFA010000000
VERSION     DADLFA010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19822
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.066"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(191..1003)
                     /locus_tag="JQH65_001986"
     CDS             complement(191..1003)
                     /locus_tag="JQH65_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HAY6139406.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGMHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKRS"
     gene            1265..2026
                     /gene="udp"
                     /locus_tag="JQH65_001987"
     CDS             1265..2026
                     /gene="udp"
                     /locus_tag="JQH65_001987"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HAY6139407.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            2167..3594
                     /gene="rmuC"
                     /locus_tag="JQH65_001988"
     CDS             2167..3594
                     /gene="rmuC"
                     /locus_tag="JQH65_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HAY6139408.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            3689..4444
                     /gene="ubiE"
                     /locus_tag="JQH65_001989"
     CDS             3689..4444
                     /gene="ubiE"
                     /locus_tag="JQH65_001989"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HAY6139409.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            4458..5063
                     /gene="ubiJ"
                     /locus_tag="JQH65_001990"
     CDS             4458..5063
                     /gene="ubiJ"
                     /locus_tag="JQH65_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HAY6139410.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            5060..6700
                     /gene="ubiB"
                     /locus_tag="JQH65_001991"
     CDS             5060..6700
                     /gene="ubiB"
                     /locus_tag="JQH65_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HAY6139411.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            6779..7048
                     /gene="tatA"
                     /locus_tag="JQH65_001992"
     CDS             6779..7048
                     /gene="tatA"
                     /locus_tag="JQH65_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HAY6139412.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            7052..7567
                     /gene="tatB"
                     /locus_tag="JQH65_001993"
     CDS             7052..7567
                     /gene="tatB"
                     /locus_tag="JQH65_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HAY6139413.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELIQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            7570..8346
                     /gene="tatC"
                     /locus_tag="JQH65_001994"
     CDS             7570..8346
                     /gene="tatC"
                     /locus_tag="JQH65_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HAY6139414.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            8388..9170
                     /gene="tatD"
                     /locus_tag="JQH65_001995"
     CDS             8388..9170
                     /gene="tatD"
                     /locus_tag="JQH65_001995"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HAY6139415.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAVQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDFTPKPSS
                     RRNEPAHLHHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(9167..9655)
                     /gene="rfaH"
                     /locus_tag="JQH65_001996"
     CDS             complement(9167..9655)
                     /gene="rfaH"
                     /locus_tag="JQH65_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HAY6139416.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            9822..11315
                     /gene="ubiD"
                     /locus_tag="JQH65_001997"
     CDS             9822..11315
                     /gene="ubiD"
                     /locus_tag="JQH65_001997"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HAY6139417.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGVPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            11361..12062
                     /gene="fre"
                     /locus_tag="JQH65_001998"
     CDS             11361..12062
                     /gene="fre"
                     /locus_tag="JQH65_001998"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HAY6139418.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDYQIVVDIPYGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLSELEALSLKHP
                     GLRVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(12254..13417)
                     /gene="fadA"
                     /locus_tag="JQH65_001999"
     CDS             complement(12254..13417)
                     /gene="fadA"
                     /locus_tag="JQH65_001999"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HAY6139419.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCN
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(13427..15630)
                     /gene="fadB"
                     /locus_tag="JQH65_002000"
                     /pseudo
     CDS             complement(13427..15630)
                     /gene="fadB"
                     /locus_tag="JQH65_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490315.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
     gene            15820..17151
                     /gene="pepQ"
                     /locus_tag="JQH65_002001"
     CDS             15820..17151
                     /gene="pepQ"
                     /locus_tag="JQH65_002001"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HAY6139420.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHASVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHLQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFSGIRIEDNVVIHENNVENMTRDLKLA"
     gene            17151..17765
                     /locus_tag="JQH65_002002"
     CDS             17151..17765
                     /locus_tag="JQH65_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HAY6139421.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGIGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            17804..19255
                     /gene="trkH"
                     /locus_tag="JQH65_002003"
     CDS             17804..19255
                     /gene="trkH"
                     /locus_tag="JQH65_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HAY6139422.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            19267..19812
                     /gene="hemG"
                     /locus_tag="JQH65_002004"
     CDS             19267..19812
                     /gene="hemG"
                     /locus_tag="JQH65_002004"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HAY6139423.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMSSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAQLTNKPTLK"
BASE COUNT         4594 a         5025 c         5427 g         4776 t
ORIGIN      
        1 gtagatggta aatctctaag gtgtcttcca ttgaagagac tcatttatga ctatgacttg
       61 ccgaccttct taaggtcaaa tagcggagtc gtaatctcca atcaattcat actgattatt
      121 ttttggggga cagagatggc agcaaaacgc actcgttcac tcaccatctc aaatatagac
      181 taagaggaaa ctaactccgc ttcccaccat actgcttaaa ccattccagc atacgctgcc
      241 agccatcttt cgcagattcg gcatgatagc tcgggcgata atcagcgttg aatgcatgcc
      301 cggcgtccgg gtacacgata atctctgctt tcgcattagc agcccgcagc gcctggcgca
      361 tggtttcaac gctctcctgc ggaatgctgt tatcctgacc accatataag ccgagaaccg
      421 gcgcgttaag atcggttgcg atatcaacag gttgtttcgg tgaatttagc gacttgtcgc
      481 ccgtcagttt gccgtaccac gccactgcag cttttagctg tggattatgc gcggcataca
      541 gccaggtgat acgtccaccc cagcagaatc cggtgatcat taaacgatga acatcgccgc
      601 cgttgcggga agcccaactg gcgacatgat cgagatcggc cagcacctgc gagtcaggca
      661 cttttgctac cagaccgcta agcaacgtgg ggatatcggc aaaatcattc ggatcgcctt
      721 cgcggaagta aagttcaggt gcgatagcca gatacccctc cagcgccaga cggcgacaaa
      781 tgtcgcggat atgttcatgc atgccaaaaa tttcctgcac tacaatgacc actggcagtg
      841 ggccatcgct ttgctttggt ctggcatggt aagcaggcat gttatcccct tgtgaaggga
      901 tagaggtgaa gcccgccaca atcgcgtcgt ccggggtctg aacgatggtc gaagcgagag
      961 gcgatgcagc aggtgcaaat ccagattgtt gtgttgttgc catggtattc tccgtaccct
     1021 tataaaaatg ttgcgcaatg ttaactatag tcagcatgca acaaatcaca ttgcctgaat
     1081 cggctcatct tttatgcagt cctgcagaat gaagagtgat ttatgtgatt tgcatcactt
     1141 ttggtgggta aatttatgca acgcatttgc gtcatggtga tgagtatcac gaaaaaatgt
     1201 taaacccttc ggtaaagtgt ctttttgctt cttctgacta aaccgattca cagaggagtt
     1261 gtatatgtcc aagtctgatg tttttcatct cggcctcact aaaaacgatt tacaaggggc
     1321 tacgcttgcc atcgtccctg gcgacccgga tcgtgtggaa aagatcgccg cgctgatgga
     1381 taagccggtt aagctggcat ctcaccgcga attcaccacc tggcgtgcag agctggatgg
     1441 taaacctgtt atcgtctgct ctaccggtat cggcggcccg tctacctcta ttgctgttga
     1501 agagctggca cagctgggca ttcgcacctt cctgcgtatc ggtacaacgg gcgctattca
     1561 gccgcatatt aatgtgggtg atgtcctggt taccacggcg tctgtccgtc tggatggcgc
     1621 gagcctgcac ttcgcaccgc tggaattccc ggctgtcgct gatttcgaat gtacgactgc
     1681 gctggttgaa gctgcgaaat ccattggcgc gacaactcac gttggcgtga cagcttcttc
     1741 tgataccttc tacccaggcc aggaacgtta cgatacttac tctggtcgcg tagttcgtca
     1801 ctttaaaggt tctatggaag agtggcaggc gatgggcgta atgaactatg aaatggaatc
     1861 tgcaaccctg ctgaccatgt gtgcaagtca gggcctgcgt gccggtatgg tagcgggtgt
     1921 tatcgttaac cgcacccagc aagagatccc gaatgctgag acgatgaaac aaactgaaag
     1981 ccaagcggtg aaaatcgtgg tggaagcggc gcgtcgtctg ctgtaattct cttctcctgt
     2041 ctgaaggccg acgcgttcgg ccttttgtat ttttgcgtag cgcctcgcag gaaatgcctt
     2101 tccaactgga cgtttgtaca gcacaattct attttgtgcg ggtaagttgt tgcgtcagga
     2161 ggcgttgtgg atttctcaat catggtttac gcagttattg cgttggtggg tgtggcaatt
     2221 ggctggctgt ttgccagtta tcaacatgcg cagcaaaaag ccgagcaatt agctgaacgt
     2281 gaagagatgg tcgcggagtt aagcgcggca aaacaacaaa ttacccaaag cgagcactgg
     2341 cgtgcagagt gcgagttact caataacgaa gtgcgcagcc tgcaaagtat taacacctct
     2401 ctggaggccg atctgcgtga agtaaccacg cggatggaag ccgcacagca acatgctgac
     2461 gataaaattc gccagatgat taacagcgag cagcgcctca gtgagcagtt tgaaaacctc
     2521 gccaaccgta tttttgagca cagcaatcgc cgggttgatg agcaaaaccg tcagagtctg
     2581 aacagcctgt tgtcgccgct acgtgaacaa ctggacggtt tccgccgtca ggttcaggac
     2641 agcttcggta aagaagcaca agaacgccat accctgaccc acgaaattcg caatctccag
     2701 caactcaacg cgcaaatggc ccaggaagcg atcaacctga cgcgcgcgct gaaaggcgac
     2761 aataaaaccc agggcaactg gggcgaggta gtattgacgc gggtgctgga ggcttccggt
     2821 ctgcgtgaag ggtatgaata tgaaacccag gtcagcatcg aaaatgacgc ccgctcgcgg
     2881 atgcagccgg atgtcatcgt gcggctgccg cagggaaaag atgtggtgat cgacgccaaa
     2941 atgacgctgg tcgcctatga acgctatttt aacgccgaag acgactacac ccgcgaaagc
     3001 gcgctacagg aacatatcgc gtcggtgcgt aaccatatcc gtttgctggg acgcaaagat
     3061 tatcaacagc tgccggggct gcgaactctg gattacgtgc tgatgtttat tcccgttgaa
     3121 cccgcttttt tactggcgct tgaccgccag ccggagctga tcaccgaagc gttgaaaaac
     3181 aatatcatgc tggttagccc gactacgctg ctggtggcgt tgcgtactat tgccaacctg
     3241 tggcgttatg agcatcaaag ccgcaacgcc cagcaaatcg ccgatcgtgc cagcaagctg
     3301 tacgacaaga tgcgtttgtt catcgatgac atgtccgcga ttggtcaaag tctcgacaaa
     3361 gcgcaggata actaccgcca ggcaatgaaa aaactctctt caggtcgcgg aaatgtgctg
     3421 gcgcaggcag aagcgtttcg cggtttagga gtagaaatta aacgcgagat taatccggat
     3481 ttggctgaac aggcggtgag ccaggatgaa gagtatcgac ttcggtcggt tccggagcag
     3541 ccgaatgatg aagcttatca acgcgatgat gaatataatc agcagtcgcg ctagcccatt
     3601 aggggtagtt aagccgggta gaaatctagg gcatcgacgc ccaatctgtt acacttctgg
     3661 aacaattttt tgatgagcag gcattgagat ggtggataag tcacaagaaa cgacgcactt
     3721 tggttttcag accgtcgcga aggaacaaaa agcggatatg gtcgcccacg ttttccattc
     3781 cgtggcatca aaatacgatg tcatgaatga tttgatgtca tttggtattc atcgtttgtg
     3841 gaagcgattc acgattgatt gcagcggcgt acgccgtggg cagaccgtgc tggatctggc
     3901 tggtggcacc ggcgacctga cagcgaaatt ctcccgcctg gtcggagaaa ctggcaaagt
     3961 ggtccttgct gatatcaatg aatccatgct caaaatggga cgcgagaagc tgcgtaatat
     4021 cggtgtgatt ggcaacgttg agtatgttca ggcgaacgct gaggcgctgc cgttcccgga
     4081 taacaccttt gattgcatca ccatttcgtt tggtctgcgt aacgtcaccg acaaagataa
     4141 agcactgcgt tcaatgtatc gcgtgctgaa acccggcggc cgcctgctgg tgcttgagtt
     4201 ctcgaagcca attatcgagc cgctgagcaa agcctatgat gcatactcct tccatgtgct
     4261 gccgcgtatt ggctcactgg tcgcgaacga cgccgacagc taccgttatc tggcagaatc
     4321 catccgtatg catcccgatc aggataccct gaaagccatg atgcaggatg ccggattcga
     4381 aagtgtcgac tactacaatc tgacggcagg ggttgtggcg ctgcatcgtg gttataagtt
     4441 ctgacaggag accggaaatg ccttttaaac ctttagtgac ggcaggaatt gaaagtctgc
     4501 tcaacacctt cctgtatcgc tcacccgcgc tgaaaacagc ccgctcgcgt ctgctgggta
     4561 aagtattgcg cgtggaggta aaaggctttt cgacgtcatt gattctggtg ttcagcgaac
     4621 gccaggttga tgtactgggc gaatgggcag gcgatgctga ctgcaccgtt atcgcctacg
     4681 ccagtgtgtt gccgaaactt cgcgatcgcc agcagcttac cgcactgatt cgcagtggtg
     4741 agctggaagt gcagggcgat attcaggtgg tgcaaaactt cgttgcgctg gcagatctgg
     4801 cagagttcga ccctgcggaa ctgctggccc cttataccgg tgatatcgcc gctgaaggaa
     4861 tcagcaaagc catgcgcgga ggcgcaaagt tcctgcatca cggcattaag cgccagcaac
     4921 gttatgtggc ggaagccatt actgaagagt ggcgtatggc acccggtccg cttgaagtgg
     4981 cctggtttgc ggaagagacg gctgccgtcg agcgtgctgt tgatgccctg accaaacggc
     5041 tggaaaaact ggaggctaaa tgacgccagg tgaagtacgg cgcctatatt tcatcattcg
     5101 cactttttta agctacggac ttgatgaact gatccccaaa atgcgtatca ccctgccgct
     5161 acggctatgg cgatactcat tattctggat gccaaatcgg cataaagaca aacttttagg
     5221 tgagcgacta cgactggccc tgcaagaact ggggccggtt tggatcaagt tcgggcaaat
     5281 gttatcaacc cgccgcgatc tttttccacc gcatattgcc gatcagctgg cgttattgca
     5341 ggacaaagta gctccgtttg atggcaagct ggcgaagcag cagattgaag ctgcaatggg
     5401 cggcttgccg gtagaagcgt ggtttgacga ttttgaaatc aagccgctgg cttctgcttc
     5461 tatcgcccag gttcataccg cgcgattgaa atcgaatggt aaagaggtgg tgattaaagt
     5521 catccgcccg gatattttgc cggtcattaa agcggatctg aaacttatct accgtctggc
     5581 tcgctgggtg ccgcgtttgc tgccggatgg tcgccgtctg cgcccaaccg aagtggtgcg
     5641 cgagtacgaa aagaccttga ttgatgaact gaatttgctg cgggaatctg ccaacgccat
     5701 tcagcttcgg cgcaattttg aagacagccc gatgctctac atcccggaag tttaccctga
     5761 ctattgtagt gaagggatga tggtgatgga gcgcatttac ggcattccgg tgtctgatgt
     5821 tgcggcgctg gagaaaaacg gcactaacat gaaattgctg gcggaacgcg gcgtgcaggt
     5881 gttcttcact caggtctttc gcgacagctt tttccatgcc gatatgcacc ctggcaacat
     5941 cttcgtaagc tatgaacacc cggaaaaccc gaaatatatc ggcattgatt gcgggattgt
     6001 tggctcgcta aacaaagaag ataaacgcta tctggcagaa aactttatcg ccttctttaa
     6061 tcgcgactat cgcaaagtgg cagagctaca cgtcgattca ggctgggtgc caccagatac
     6121 caacgttgaa gagttcgaat ttgccattcg tacggtctgt gaacctatct ttgagaaacc
     6181 gctggccgaa atttcgtttg gacatgtact gttaaatctg tttaatacgg cgcgtcgctt
     6241 caatatggaa gtgcagccgc aactggtgtt actccagaaa accctgctct atgtcgaagg
     6301 ggtaggacgc cagctttatc cgcagctcga tttatggaaa acagcgaagc ctttcctgga
     6361 gtcgtggatt aaagatcagg tcggtattcc tgcgctggtg agagcattta aagaaaaagc
     6421 gccgttctgg gtcgaaaaaa tgccagaact gcctgaattg gtttacgaca gtttgcgcca
     6481 gggcaagtat ttacagcaca gtgttgataa gattgcccgc gagcttcagt caaatcatgt
     6541 acgtcaggga caatcgcgtt attttctcgg aattggcgct acgttagtat taagtggcac
     6601 attcttgttg gtcagccgac ctgagtgggg gctgatgccc ggctggttaa tggcaggtgg
     6661 tctgatcgcc tggtttgtcg gttggcgcaa aacacgctga ttttttcatc gctcaaggcg
     6721 ggccgtgtaa cgtataatgc ggctttgttt aatcatcatc taccacagag gaacatgtat
     6781 gggtggtatc agtatttggc agttattgat tattgccgtc atcgttgtac tgctttttgg
     6841 caccaaaaag ctcggctcca tcggttccga tcttggtgcg tcgatcaaag gctttaaaaa
     6901 agcaatgagc gatgatgaac caaagcagga taaaaccagt caggatgctg attttactgc
     6961 gaaaactatc gccgataagc aggcggatac gaatcaggaa caggctaaaa cagaagacgc
     7021 gaagcgccac gataaagagc aggtgtaatc cgtgtttgat atcggtttta gcgaactgct
     7081 attggtgttc atcatcggcc tcgtcgttct ggggccgcaa cgactgcctg tggcggtaaa
     7141 aacggtagcg ggctggattc gcgcgttgcg ttcactggcg acaacggtgc agaacgaact
     7201 gatccaggag ttaaaactcc aggagtttca ggacagtctg aaaaaggttg aaaaggcgag
     7261 cctcactaac ctgacgcccg aactgaaagc gtcgatggat gaactacgcc aggccgcgga
     7321 gtcgatgaag cgttcctacg ttgcaaacga tccagaaaag gcgagcgatg aagcgcacac
     7381 catccataac ccggtggtga aagataatga agctgcgcat gagggcgtaa cgcctgccgc
     7441 tgcacaaacg caggccagtt cgccggaaca gaagccagaa accacgccag agccggtggt
     7501 aaaacctgct gcggacgctg aaccgaaaac cgctgcacct tccccttcgt cgagtgataa
     7561 accgtaaaca tgtctgtaga agatactcaa ccgcttatca cgcatctaat tgagctgcgt
     7621 aagcgtctgc tgaactgcat tatcgcggtg atcgtgatat tcctgtgtct ggtctatttc
     7681 gccaatgaca tctatcacct ggtatccgcg ccattgatca agcagttgcc gcaaggttca
     7741 acgatgatcg ccaccgacgt ggcctcgccg ttctttacgc cgatcaagct gacctttatg
     7801 gtgtcgctga ttctgtcagc gccggtgatt ctctatcagg tgtgggcatt tatcgcccca
     7861 gcgctgtata agcatgaacg tcgcctggtg gtgccgctgc tggtttccag ctctctgctg
     7921 ttttatatcg gcatggcatt cgcctacttt gtggtctttc cgctggcatt tggcttcctt
     7981 gccaataccg cgccggaagg ggtgcaggta tccaccgaca tcgccagcta tttaagcttc
     8041 gttatggcgc tgtttatggc gtttggtgtc tcctttgaag tgccggtggc aattgtgctg
     8101 ctgtgctgga tggggattac ctcgccagaa gacttacgca aaaaacgccc gtatgtgctg
     8161 gttggtgcat tcgttgtcgg gatgttgctg acgccgccgg atgtcttctc gcaaacgcta
     8221 ttggcgatcc cgatgtactg tctgtttgaa atcggtgtct tcttctcacg cttttacgtt
     8281 ggtaaagggc gaaatcggga agaggaaaac gacgctgaag cagaaagcga aaaaactgaa
     8341 gaataaattc aaccgcccgt cagggcggtt gtcatatgga gtacaggatg tttgatatcg
     8401 gcgttaattt gaccagttcg caatttgcga aagaccgtga tgatgttgta gcgcgcgctt
     8461 ttgacgcggg agttaatggg ctactcatca ccggcactaa cctgcgtgaa agccagcagg
     8521 cgcaaaagct ggcgcgtcag tattcgtcct gttggtcaac ggcgggcgta catcctcacg
     8581 acagcagcca gtggcaagct gcgactgaag aagcgattat tgagctggcc gtgcagccag
     8641 aagtggtggc gattggtgaa tgtggtctcg actttaaccg caacttttcg acgccggaag
     8701 agcaggaacg cgcttttgtt gctcagctac gcattgccgc agaattaaac atgccggtat
     8761 ttatgcactg tcgcgatgcc cacgagcggt ttatgacatt gctggagccg tggctggata
     8821 aactgcctgg tgcggttctt cattgcttta ccggcacacg cgaagagatg caggcgtgcg
     8881 tggcgcgtgg aatttatatc ggcattaccg gttgggtttg cgatgaacga cgcgggctag
     8941 agctgcggga attgttaccg ttgattccgg cggagaaatt gctgatcgaa actgatgcgc
     9001 cgtatctgct cccgcgcgat ttcacgccaa agccatcatc ccggcgcaac gagccagccc
     9061 atctgcacca tattttgcaa cgtattgcgc actggcgtgg agaagatgcc gcatggctgg
     9121 ctgccaccac ggatgccaat gtcaaaacac tgtttgggat tgcgttttag agtttgcgga
     9181 actcggtgtt cttcacactg tgcttaatct ctttattaat aagattaagc aatagcatgg
     9241 aacgagcctc accatcgggt tcggtgaaaa tggcctgaaa gccttcgaac gcgccttcgg
     9301 taataatcac cttatcaccc ggataaggtg ttgccggatc gacaatgtct ttcggtttat
     9361 ataccgatag ctgatgaatc accgccgatg ggactatcgc tggcgacgcg ccaaagcgca
     9421 cgaagtggct gacaccgcgg gtcgcgttga tagtcgtggt atgaatcact tctggatcaa
     9481 attcgacaaa caggtagttg ggaaacaatg gctcactgac tgcagtacgt tttccacgca
     9541 cgattttttc cagggtgatc atcggtgcca ggcaattcac agcctgtctt tcgaggtgtt
     9601 cctgggcacg ttgaagttgc ccgcgcttgc agtacagtaa ataccaggat tgcataatga
     9661 ctcttatccg tttaatcggg gcgcaaggat agcaaaagct ttacgctaag ttaattatat
     9721 tccccggttt gcgttatacc gtcagagttc acgctaattt aacaaattta cagcatcgca
     9781 aagatgaacg ccgtataatg ggcgtagatt aagaggctac aatggacgcc atgaaatata
     9841 acgatttacg cgacttcttg acgctgcttg aacagcaggg tgagctaaaa cgtatcacgc
     9901 tcccggtgga tccgcatctg gaaatcactg aaattgctga ccgcactttg cgtgccggtg
     9961 ggcctgcgct gttgttcgaa aaccctaaag gctactcaat gccggtgctg tgcaacctgt
    10021 tcggtacgcc aaagcgcgtg gcgatgggca tgggacagga agatgtttcg gcgctgcgtg
    10081 aagttggtaa attattggcg tttctgaaag agccggagcc gccaaaaggt ttccgcgacc
    10141 tgtttgataa actgccgcag tttaagcaag tattgaacat gccgacaaag cggctgcgtg
    10201 gtgtgccctg ccaacaaaaa atcgtctctg gcgatgacgt cgatctcaat cgcattccca
    10261 ttatgacctg ctggccggaa gatgccgcac cgctgattac ctgggggctg acagtgacgc
    10321 gcggcccgca taaagagcgg cagaatctgg gcatttatcg ccagcagctg attggtaaaa
    10381 acaaactgat tatgcgctgg ctgtcgcatc gcggcggcgc gctggattat caggagtggt
    10441 gtgcggcgca tccgggcgaa cgtttcccgg tttctgtggc gctgggtgcc gatcccgcca
    10501 cgattctcgg tgcagtcacc cccgttccgg atacactttc agagtatgcg tttgccggat
    10561 tgctgcgcgg taccaagacc gaagtggtga agtgtatctc caatgacctt gaagtgcccg
    10621 ccagtgcgga gattgtgctg gaagggtata tcgaacaagg cgaaactgcg ccggaagggc
    10681 cgtatggcga ccacaccggt tactataacg aagtcgatag tttcccggta tttaccgtga
    10741 cgcatattac ccagcgtgaa gatgcgattt accattccac ctataccggg cgtccgccag
    10801 atgagcccgc ggtactgggc gtggcgttga acgaagtgtt tgtaccgatt ctgcaaaaac
    10861 agttcccgga aattgtcgat ttttacctgc cgccggaagg ctgttcttat cgtctggcgg
    10921 tagtgacgat caaaaaacag tacgccggac acgcgaagcg cgtcatgatg ggcgtctggt
    10981 cgttcttacg ccagtttatg tacactaaat ttgtgatcgt ttgcgatgat gacgtcaacg
    11041 cacgcgactg gaacgatgtg atttgggcga ttaccacccg tatggacccg gcgcgggata
    11101 ctgttctggt agaaaatacg cctattgatt atctggattt tgcctcgcct gtctccgggc
    11161 tgggttcaaa aatggggctg gatgccacga ataaatggcc gggggaaacc cagcgtgaat
    11221 ggggacgtcc catcaaaaaa gatccagatg ttgtcgcgca tattgacgcc atctgggatg
    11281 aactggctat ttttaacaac ggtaaaagcg cctgatgcgc gtttgttttg ccctatttat
    11341 cgatccgaca gagaaagcgc atgacaacct taagctgtaa agtgacctcg gtagaagcta
    11401 tcacggatac cgtatatcgt gtccgcatcg tgccagacgc ggccttttct tttcgtgctg
    11461 gtcagtattt gatggtagtg atggatgagc gcgacaaacg tccgttttca atggcttcga
    11521 cgccggatga aaaagggttt atagagctgc atattggcgc ttctgaaatc aacctttacg
    11581 cgaaagcagt catggaccgc atcctcaaag attatcaaat cgtggtcgat attccctacg
    11641 gagaagcgtg gctgcgcgat gatgaagagc gtccgatgat tttgattgcg ggcggcaccg
    11701 ggttctccta tgcgcgctcg attttattga cggcgttggc gcgtaatccc aatcgcgata
    11761 tcaccattta ctggggtggg cgtgaggagc aacatcttta tgatctctcc gagcttgagg
    11821 cgctttcgct gaagcatcct ggcctgcgtg tggtgccggt ggttgaacaa ccggaagcgg
    11881 gctggcgtgg gcgtactggc accgtgttaa cggcggtctt gcaggatcat ggtacgctgg
    11941 cagagcatga tatctatatt gccggacgtt ttgagatggc gaaaattgct cgtgacctgt
    12001 tttgcagtga gcgtaatgcg cgggaagatc gtctgtttgg cgatgcgttt gcatttatct
    12061 gagatataaa aaaacccgcc cctgacaggc gggaagaacg gcaactaaaa ctttattcag
    12121 tggcatttag atctatgtcg catccggcaa tcggtgcacg atgcctgatg cgacgctggc
    12181 gcgtcttatc aggcctacat tggtgcagga tcggtagacc ggataaggcg ttcacgccgc
    12241 atccggcaag tggttaaacc cgctcaaaca ccgtcgcaat accctgaccc agaccgatac
    12301 acatcgtcgc cagaccaaac tgaacgtctt tgcgttccat cagattcagc agcgtggtgc
    12361 tgatacgcgc accggaacag cccagcggat gacccagcgc gatcgcacca ccattgaggt
    12421 tgatcttctc gtcaatctgc tccattagcc cgagatcttt aatgcacggc aggatctgcg
    12481 cggcaaaggc ttcgttcatc tcaaacacgc cgatatcgct ggcagaaagc cccgcttttt
    12541 tcagcgccag cttcgaggcc ggaaccgggc cgtaacccat aatcgatggg ttacaaccaa
    12601 cgaccgccat cgaacgcaca cgagcgcgcg gcttaagacc taattcatgg gcgcggcttt
    12661 cactcatcac cagcatggca gctgcgccat cggaaagtgc agaagatgtg cccgccgtta
    12721 ccgtaccgtt tactggatca aacgccggac gcagcgtggc gagggcttcc acggtggttt
    12781 ccggtcgaat cacctcgtcg taattaaact gcttcaggac gccgtcggca tcgtgaccac
    12841 cggtcgggat gatttcattt ttaaatgcgc ccgactgcgt ggcggcccag gcgcgggcgt
    12901 gtgaccgcgc ggcaaaggca tcctgcattt cacggctgat accgtgcata cgcgccagca
    12961 tttctgccgt taagcccatc atgcccgccg ctttggcgac attgcggctc aggccggggt
    13021 gaaaatcgac gccgtgactc attggcacat ggcccatatg ctccacgccg ccaaccagac
    13081 atgcctgcgc atcgccagtc atgatcattc gtgctgcgtc atgcagtgcc tgcatggatg
    13141 aaccacacaa gcgattgacg gtaaccgccg ggacagagtg cggcacttct gccagcagcg
    13201 ccgcgttacg ggcgatatta aaaccctgct ccagcgtctg ctgcacacaa ccccagtaaa
    13261 tatcgtcgag ggccgccgct tccagcgccg ggttacgcgc cagcaggcta cgcattaaat
    13321 gagcggagag atcttctgca cgcacgttac gaaaagcacc gcccttcgaa cggcccatcg
    13381 gggtgcgaat tgcatcgaca atgacaacct gttccattgt gactccttaa gccgttttca
    13441 ggtcgccaac cggacgggct ggctcaaccg gaggatagta cggttcgtta tgacgcgctt
    13501 tattacgcag accttccggc acttcataca gcgggccgag gtgctgatat tgctgtgcca
    13561 tatcgaggta ttttgcgcta ccgagggtgt ccagccagcg gaacgcgccg ccgtggaacg
    13621 gagggaagcc caggccgtag accagcgcca tatccgcttc cgccggagtg gcgataatgc
    13681 cctcttccag acagcggacc acttcgttga ccatcgggat catcatgcgg gcgataatct
    13741 cttcttcgct gaaatcgcgc ttcggctggc tcacttctgc cagcaggtct tcaacggcgg
    13801 cgtcttcttc tttcttcggc ttacctttgc tgtcttcttt ataacgccag aaaccgaggc
    13861 cgttcttctg accaaagcgg ttggcatcaa acagcgcgtc gatggcatcg cggtaatctt
    13921 tctgcatccg ctgcgggaag cctgctgcca tgacagcctg agcgtgatgc gcggtatcaa
    13981 tgcccacgac atccagcaga tatgccggac ccataggcca gccaaactgt ttttccatca
    14041 ctttgtcgat cttgcggaaa tccgcgccgt cgcgcagcag ctggctgaaa ccggcgaaat
    14101 acgggaacag cacgcggtta acaaagaagc cggggcagtc gttaaccaca atcggcgtct
    14161 tgcccatctt gctcgcccag gcgacaactt tcgcgatggt ttcgtcggag cttttctcgc
    14221 cgcgaataat ttctaccaac ggcatccggt gtaccgggtt aaaaaagtgc attccgcaga
    14281 agttttccgg gcgttccagc gcgttggcca gttcgctgat aggaatggtt gaagtgttag
    14341 acgccagtac ggtatccggg cgtacttttt gttcggtttc tgccagtacg gcttttttca
    14401 ctttcgggtt ttcgacaacc gcttctacca caacatccac gcggtcaaac ccggcgtagt
    14461 cgagcgttgg gtggatcgtg gagatcacgc cagccagttt cagaccatcg atcttgccgc
    14521 gttcaagctg cttgttcagc agtttcgcgg cttcggtcat gccgagggtt aacgacttgt
    14581 cgttgatatc tttcatgaca accggtacgc ctttccacgc agactggtaa gcaatgccgc
    14641 cacccatgat gcctgcgccc agtacagcgg cctgtttcgg tgtttcaacg tctttggtga
    14701 gtttcttcgc tttgcctttt acatattgat cgttaaggaa aatgccgacc agtgcgcggg
    14761 cttcgttggt gtgcgccagc gggacaaagc ttttgttttc cagatttaat gcttcttcac
    14821 gaccaaaacg ggccgcagct tcaatggttt ttactgcggt gatgggcgcc ggataatgtt
    14881 tccccgccgt ttgtgcgacc atccctttag cgatagtgaa gctcatggtg gcttcaatct
    14941 tgctcagttt tagtggttcc agcttcggct gacgttttgc tttccagtcg aggtcgccgt
    15001 taatggcctg gcgtaaaacc gcctttgcgc cttcaaccag tttttctgct ttgactacgc
    15061 catccaccag accgattttc agcgcctgat ccgcgccgac atctttaccg gcggcaatga
    15121 tttccagcgc actgtcagcg cccagcatac gtggcatacg tacagaaccg ccaaagcccg
    15181 gcatgatgcc cagtttggtt tccggcagac cgatgcgcag atccggcgtc gccagacgat
    15241 aatcggtcgc cagcacgcat tcgcagccac cacccagcgc atagccgttg acggcagcaa
    15301 tggtcggcac cggcagatct tccaagcgat taaacacgct attggcaaag tgcagccact
    15361 gacttaactg ttcttcagga acgaggaacg aggaacgagg aacagggaca aaaattcggt
    15421 gatatcagca ccgacgataa aggctgcttt gttcgaacgc agcagcagcc cttttagatc
    15481 tgattgctgt tccagcacgc cgatggcctc gccgaggctg gcgacggtcg cagtgtcgag
    15541 tttattaact gaacctgggg catcaaatac cagttcggca atgccatctt ccagccagtc
    15601 aaggtacagg gtgtcgcctt tgtaaagcat gtcagtctcc tgaatccgca aggtgatctg
    15661 gtcgtaccag atgcatcgaa gtgtgtattt tgtgttaaaa acatgtaaat aaaagattaa
    15721 agaaatgccg atctgatcac gctcggcaga aatcacgctc tggatgaacg atgtgctaag
    15781 atgcggagac ttaaggtcaa aaaaacagaa gggtaaaaaa tggaatcact ggcctcgctc
    15841 tataaaaatc atatagctac cttacaagaa cggactcgcg atgcgctggc gcgcttcaag
    15901 ctggatgcat tacttattca ctccggcgag ctgttcaacg tttttctcga cgatcatccc
    15961 tatccgttta aagtgaaccc gcaattcaaa gcgtgggtgc cggtaactca ggtgccaaac
    16021 tgctggttgc tggtggatgg cgtgaacaag ccgaaactgt ggttctatct gccggttgat
    16081 tactggcaca acgtcgaacc gctgccgacc tccttctgga ctgaagatgt ggaagtgatc
    16141 gcactgccga aagccgatgg cattggtagc ctgctgcccg ctgcgcgcgg caatatcggt
    16201 tatatcggtc cggtgccgga acgtgcgctg caactgggta ttgaggccag caacatcaac
    16261 ccgaaagggg tgatcgacta cctgcattac taccgctcct tcaaaaccga gtacgaactg
    16321 gcctgtatgc gtgaagcgca gaaaatggcg gtcaacggtc atcgtgcggc agaagaagcg
    16381 ttccgttctg gcatgagcga gttcgatatc aacatcgcct atctgaccgc gaccggtcat
    16441 cgtgataccg acgtacctta cagcaacatt gtggcgctta acgaacacgc ttcggtgctg
    16501 cattacacca aactggatca tcaggcaccg gaagagatgc gcagcttcct gctggatgcc
    16561 ggggccgaat ataacggcta tgcggctgac ctgactcgta cctggtcggc aaaaagcgac
    16621 aacgactacg cacagctggt gaaagacgta aatgatgaac aacttgcgct gatcgctacc
    16681 atgaaagctg gcgtcagcta tgtggattac cacctccagt tccatcagcg catcgccaaa
    16741 ttgctgcgta aacatcaaat catcaccgat atgagtgaag aagcgatggt cgaaaacgat
    16801 ctcaccggac cgtttatgcc gcacggtatc ggccatccgc tgggcctgca ggtgcatgac
    16861 gtagccggtt ttatgcagga tgatagcggt acacacctcg cggcaccggc aaaatatccg
    16921 tacctgcgct gcacccgtat tctccagccg ggcatggtgt taaccatcga accgggtatc
    16981 tacttcatcg aatcgctact ggcaccgtgg cgtgaagggc agttcagcaa gcacttcaac
    17041 tggcagaaaa ttgaagcact gaaaccgttc agcggcattc gtatcgaaga caacgtggtg
    17101 atccacgaaa ataacgtgga aaacatgacc cgggatctga aactggcgtg atggaaagct
    17161 ggttaattcc tgcggcaccg gtcacggtcg ttgaagagat caaaaagagc cgcttcataa
    17221 cgatgttggc gcataccgat ggcgttgagg cggcgaaagc gtttgttgaa tcggtgcggg
    17281 cagaacaccc cgatgcccgc caccattgcg tggcgtgggt ggcgggtgcg ccggatgatt
    17341 ctcaacagct gggtttctct gacgacgggg agccggcggg aacggcaggt aaaccgatgc
    17401 tcgcccagct aatgggcagc ggcatcgggg aaattaccgc tgtggtagtg cgctactacg
    17461 gcggcatatt gcttggcacc ggtgggttag tgaaagcgta tggcggcggc gtgaatcagg
    17521 cgctgcgcca gctaacgacc caacgcaaga cgccattaac cgaatatact ttgcaatgtg
    17581 aatatagtca gttaaccggc attgaagcgt tgctggggca gtgtgacggc aaaattatca
    17641 acagtgatta tcaggcattc gttctgctgc gggtggcgct tccggcggcg aaagtggctg
    17701 aattttccgc aaagctggcg gattttagcc gtggttcatt gcaattgtta gcgattgaag
    17761 aataatcccc acttcatttt gcagaactaa ggaagcggca gagatgcatt ttcgcgccat
    17821 tacccgaatc gttggactac tggtcatctt attttcaggg accatgatta tccctgggct
    17881 ggtagcactc atctaccggg atggagcggg ccgcgctttt acccagacct tttttgtcgc
    17941 cctcgccatt ggctctatgc tgtggtggcc gaaccgcaaa gagaagggcg aactgaaatc
    18001 ccgtgagggg tttctgatag tggtgctgtt ttggaccgtg ctgggtagtg tcggtgcgct
    18061 cccttttatc ttctcggaaa gcccgaacct cacgattacc gatgcgtttt ttgaatcttt
    18121 ctctggcctg accaccaccg gggccactac gctggtgggg ctggattcgc tccctcatgc
    18181 catcctcttt tatcgccaga tgctgcaatg gtttggcggg atggggatca tcgtgttagc
    18241 ggttgcgata ctgcctatcc tcggcgtggg tgggatgcag ctctatcgcg cagaaatgcc
    18301 cggcccgctg aaagataaca aaatgcgccc gcgaattgcg gaaacggcga aaaccctgtg
    18361 gttgatttat gtcttgctga ccgtcgcctg tgcgctggcg ttgtggtttg ccggaatgga
    18421 tgcctttgat gccatcggcc atagctttgc gactatcgct attggcggct tctcgacaca
    18481 tgatgccagt atcggttatt tcgacagtcc gactattaac actatcattg ctatcttcct
    18541 gctgatctcc ggttgtaact acggtctgca cttttcactg ttaagtgggc gtagtctgaa
    18601 ggtttattgg cgcgatccgg aatttcgcat gtttatcggc gtacagttta cgctggtggt
    18661 tatttgtaca ctcgtactgt ggtttcataa tgtctacagt tcggcgctga tgacaattaa
    18721 ccaggcgttt ttccaggtgg tatcgatggc gacaaccgcc gggtttacga ctgacagcat
    18781 tgcccgctgg ccgctctttt tgccggtact gcttttatgt tcagctttta tcggcggttg
    18841 cgccgggtca acgggcggtg gcctgaaagt gatccgcatc ctgctgctgt ttaagcaggg
    18901 gaaccgtgag ctgaaacgac tggtgcatcc gaacgccgtc tatagcatta agctggggaa
    18961 tcgcgcactg ccggaacgta tcctcgaagc cgtttgggga tttttctccg cctatgcatt
    19021 ggtgtttatt gtcagtatgc tggcgattat cgccacgggc gtggatgatt tttctgcctt
    19081 tgcctcagtt gttgcgacat tgaataacct ggggccaggg cttggcgtgg ttgctgataa
    19141 ctttaccagt atgaacccgg tggctaaatg gatcctgatt gccaacatgc tgtttggtcg
    19201 tctcgaggtc tttacattgc tggtgctctt taccccgact ttctggcgtg aatgatggag
    19261 taatacgtga aaacattaat tcttttctca acaagggacg gacaaacgcg cgagattgcc
    19321 tcctacctgg cttcggaact gaaagaactg gggatccaga cggatgtcgc caatgtgcac
    19381 cgcattgaag aaccacaatg ggaaaactat gaccgtgtgg tcattggtgc ttctattcgc
    19441 tatggtcact accactctgc gttccaggaa tttgtcaaaa aacatgcgac gcgactgaat
    19501 tcgatgccga gcgcctttta ttccgtaaac ctggtggcgc gcaaaccgga gaagcgtact
    19561 ccacagacca acagctacgc gcgcaagttt ctgatgagtt cacaatggcg tcccgatcgc
    19621 tgcgcggtca ttgccggggc gctgcgttac ccacgttatc gctggtacga ccgttttatg
    19681 attaagctga ttatgaagat gtcaggcggt gaaacggata cgcgcaaaga agttgtctat
    19741 accgattggg agcaggtggc gaatttcgcc cgagaaatcg cccagttaac caacaaaccg
    19801 acgctgaaat aagcataaag aa
//
LOCUS       DADLFA010000067        19367 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000067 DADLFA010000000
VERSION     DADLFA010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19367
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.067"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(31..1860)
                     /gene="glmS"
                     /locus_tag="JQH65_002005"
     CDS             complement(31..1860)
                     /gene="glmS"
                     /locus_tag="JQH65_002005"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAY6139424.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIMVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2022..3392)
                     /gene="glmU"
                     /locus_tag="JQH65_002006"
     CDS             complement(2022..3392)
                     /gene="glmU"
                     /locus_tag="JQH65_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAY6139425.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILISNGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDVNLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3743..4162)
                     /gene="atpC"
                     /locus_tag="JQH65_002007"
     CDS             complement(3743..4162)
                     /gene="atpC"
                     /locus_tag="JQH65_002007"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAY6139426.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQSGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4183..5565)
                     /gene="atpD"
                     /locus_tag="JQH65_002008"
     CDS             complement(4183..5565)
                     /gene="atpD"
                     /locus_tag="JQH65_002008"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAY6139427.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5592..6455)
                     /gene="atpG"
                     /locus_tag="JQH65_002009"
     CDS             complement(5592..6455)
                     /gene="atpG"
                     /locus_tag="JQH65_002009"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAY6139428.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHLYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6506..8047)
                     /gene="atpA"
                     /locus_tag="JQH65_002010"
     CDS             complement(6506..8047)
                     /gene="atpA"
                     /locus_tag="JQH65_002010"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAY6139429.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8060..8593)
                     /gene="atpH"
                     /locus_tag="JQH65_002011"
     CDS             complement(8060..8593)
                     /gene="atpH"
                     /locus_tag="JQH65_002011"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAY6139430.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8608..9078)
                     /gene="atpF"
                     /locus_tag="JQH65_002012"
     CDS             complement(8608..9078)
                     /gene="atpF"
                     /locus_tag="JQH65_002012"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAY6139431.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9140..9379)
                     /gene="atpE"
                     /locus_tag="JQH65_002013"
     CDS             complement(9140..9379)
                     /gene="atpE"
                     /locus_tag="JQH65_002013"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HAY6139432.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9426..10241)
                     /gene="atpB"
                     /locus_tag="JQH65_002014"
     CDS             complement(9426..10241)
                     /gene="atpB"
                     /locus_tag="JQH65_002014"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAY6139433.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10250..10630)
                     /gene="atpI"
                     /locus_tag="JQH65_002015"
     CDS             complement(10250..10630)
                     /gene="atpI"
                     /locus_tag="JQH65_002015"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HAY6139434.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11247..11870)
                     /gene="rsmG"
                     /locus_tag="JQH65_002016"
     CDS             complement(11247..11870)
                     /gene="rsmG"
                     /locus_tag="JQH65_002016"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAY6139435.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(11934..13823)
                     /gene="mnmG"
                     /locus_tag="JQH65_002017"
     CDS             complement(11934..13823)
                     /gene="mnmG"
                     /locus_tag="JQH65_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAY6139436.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14202..14645)
                     /gene="mioC"
                     /locus_tag="JQH65_002018"
     CDS             complement(14202..14645)
                     /gene="mioC"
                     /locus_tag="JQH65_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HAY6139437.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(14735..15193)
                     /gene="asnC"
                     /locus_tag="JQH65_002019"
     CDS             complement(14735..15193)
                     /gene="asnC"
                     /locus_tag="JQH65_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HAY6139438.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQHPIMRTIKP"
     gene            15345..16337
                     /gene="asnA"
                     /locus_tag="JQH65_002020"
     CDS             15345..16337
                     /gene="asnA"
                     /locus_tag="JQH65_002020"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HAY6139439.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYLDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(16342..17793)
                     /gene="viaA"
                     /locus_tag="JQH65_002021"
     CDS             complement(16342..17793)
                     /gene="viaA"
                     /locus_tag="JQH65_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HAY6139440.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(17787..19283)
                     /gene="ravA"
                     /locus_tag="JQH65_002022"
     CDS             complement(17787..19283)
                     /gene="ravA"
                     /locus_tag="JQH65_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HAY6139441.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEVEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
BASE COUNT         4631 a         5118 c         4848 g         4770 t
ORIGIN      
        1 tcggcagccc ttttttatct atgatagtga ttactcaacc gtaaccgatt ttgccaagtt
       61 acgcggctgg tcaacgtcgg tgcctttgat cagcgcgaca tggtaagcca gcagctgcag
      121 cggaacggtg tagaagatcg gtgcaatcac ctcttccaca tgcggcatct cgatgatgtg
      181 catgttatcg ctacttacaa aacccgcatc ctgatcggcg aagacataca actgaccgcc
      241 acgcgcgcga acttcttcaa tgttggattt cagtttttcc aacagttcgt tgttcggtgc
      301 aacgacgata accggcatat ccgcgtcaat cagcgccagc gggccgtgtt tcagctcgcc
      361 agcagcgtag gcttcagcgt gaatgtagga gatctctttc agcttcaatg cgccttccag
      421 tgcgattggg tactgatcgc cacggcccag gaacagtgcg tgatgtttgt cagagaaatc
      481 ttctgccaga gcttcaatgc gtttgtcctg agatagcatc tgctcaatac ggctcggcag
      541 cgcctgcaga ccatgcacaa tgtcatgttc aatggaggca tccagacctt tcaggcgaga
      601 cagcttcgcc accagcatca acagcacagt taactgagtg gtgaatgctt tagtggacgc
      661 cacgccgatt tccgtacccg cgttggtcat cagcgccaga tcggattcgc gcaccagaga
      721 agaacccgga acgttacaga ttgccagcga accaaggtaa cccagctctt tcgacagacg
      781 caggcccgcc agggtatccg cggtttcgcc agactgtgac aaggtgatca tcaggctgtt
      841 acgacgcacg gcagatttac gatagcggaa ttcagaggcg atttcgacgt cgcacggaat
      901 acctgccagc gattcaaacc agtagcggga aaccataccg gagttataag aagtaccaca
      961 ggcgaggatc tgaatatgct caaccttcga cagcagttcg tcggcgttcg gtcccagctc
     1021 gcttaaatca acctgaccgt ggctgatgcg cccagtaagg gtgtttttga tcgcgttcgg
     1081 ctgttcgtag atctctttct gcatgtagtg acggtaaata cctttatcgc ccgcgtcata
     1141 ttgcagattg gattcgatat cctgacgttt tacttccgcg ccagttttat cgaagatgtt
     1201 taccgaacgg cgagtaattt ccgcaatatc gccctcttca aggaagataa agcgacgggt
     1261 caccggcagc agcgccagct ggtcagaagc gataaagttt tcgcccatac ccaggccaat
     1321 caccagcgga ctaccagaac gtgccgccag cagggtatcc gggtgacggg agtccatgat
     1381 cactgtaccg tacgcaccgc gcagctgcgg gatagcacgc agaacagcct cacgcagagt
     1441 cccgccttgt tttagctccc agttcaccag atgggcaatc acttcggtgt cggtttcaga
     1501 aacgaaggta tagccacgcg cttttaactc ttcacgcagc ggttcatggt tttcgatgat
     1561 gccgttatgc accaccataa tgtgttcaga aacatgcgga tgcgcgttag cttctgaagg
     1621 ttcaccgtgg gtcgcccagc gagtgtgagc aataccggtg ccgccatgca gaggatgttc
     1681 ttccgctgcc tgagccagca tctggacttt accgaggcga cgcaggcggg tcatatgacc
     1741 ttccgcatca acaacggcca gaccagcaga gtcatatccg cggtattcca gacgacgtaa
     1801 accttcaaga aggatttctg ctacatcacg ttgcgcgatc gcgccaacaa ttccacacat
     1861 agtttttgat tccgatttat atcgttgtcg gtcaacctgt atgcccgttt tttcgggcgc
     1921 cccgagcctt gtagagagtg gggttatttt tatagttact gcttgtggga gggagattat
     1981 gttatctcct catcccagat gaccggatta cccggccaga attacttttt ctttaccgga
     2041 cgacgccagc cttctttctg agtctgcggc acacggctga tagctaatgc attttcgccg
     2101 acattacgcg tcacagttgt acccgcagca atggtcgcgc ctttgcctac tgttaccggg
     2161 gccaccagct gagtgtcgga accgacaaac acatcgtcgc cgataatggt cttaaattta
     2221 ttcgcaccat cgtagttgca ggtaatggtt cccgcgccga tgttaacgtt atcgccaatt
     2281 tccgcatcgc ccaggtaagt cagatgacca gctttcgagc ctttacccag acgcgctttt
     2341 ttcatctcaa caaagttacc gacgtgtgca ccttccagca actcagcacc aggacgcaga
     2401 cgggcaaacg ggccaatagt acaggccgct gccagattca cgtcttccac aacggtatac
     2461 ggactgattt cgcaatcatc gccaatcacg ctgtttttaa tcacgcaacc ggtgccgatt
     2521 ttcacgcgat gaccgagagt cacgttgccc tcgatgataa cgttagtatc aatttcaaca
     2581 tcgcgcccgt gagtaagcgt accgcgcaga tcaaaacgcg ccggatcgcg cagcataacg
     2641 cctgctaaca gcagtttttc agcctgttcg gactggtaaa cgcgctccag acgggagagt
     2701 tgcaggcggt tattcacacc ttctacttcg cttaaacgtt gcggatgaac ggcgacgatt
     2761 tcacgccctt cctgatacgc cagcgcaata atgtcggtga tgtagtattc gccctgagca
     2821 ttattgttgg tcagcttcgc cagccagcgt ttcatatctg cgccgttaga gatcagaatg
     2881 ccggtgttga tctcctgaat ctgacgctgc tcgtcggtgg catctttgtg ctcaacaatg
     2941 ccggtaactt tgccattttc acgggtgata cgtccataac cggtcggatc atccagtttc
     3001 accgtcagca gaccaatgcc accctgcggt ttagcatcac gcaggcgctg gagtgtttcg
     3061 acagagatca gcggcacgtc gccgtagagc attaaaatgt cttcatcatc ggcaaagaaa
     3121 ggtgcggcct gctgcattgc atgacccgta cccagctgct ctgcctgaag cacccagtta
     3181 aggttgtcgt ctttcagcgc ctgtttaagc agatcgccgc cgtgaccgta caccaggtga
     3241 acgtgcgctg cgcctaattc attcgcagca tcaatgacat gctgaaccat cgctttcccg
     3301 gcaagggtat gcagcacttt cggaagatcg gaatacatgc gcgtgccttt gcctgcggca
     3361 aggatcacta cgctcatagc attattcaac atacgcgtcc tgactgtaat ttgagaacga
     3421 atttaaaccg cttcaccttg aaaaaactac atttttttca tcgtgaaatg gacagaggat
     3481 aaattgttca ataacggatt atccccgctg acgaaagtgc cattttcgac catcgtcacg
     3541 ccttttgtct gcagaaaatt aaggcagtac cttcatttaa gcgtcaggtg gatgtttttg
     3601 ctcttatttc gatcaatgaa taaacgggaa aacaggggtg ttttatatca gcaggatcta
     3661 tgtgaacgct cttcaggacg gatcacgcgt aaaaaaaaac cagcctgttt ccagactggc
     3721 ttttgtgctt ttcaagccgg tgttacatcg cttttttggt caactcgata acgcgcagct
     3781 gcgcgatcgc tttggccagt tccgcagacg cctgagcgta atctacgtcg ccgtgagagc
     3841 tgctaatgtg ctcttcagcc ttacgtttcg cttccatggc tcgcgcttcg tcgagatcct
     3901 ggccgcgaat tgcggtgtcg gccagaacgg tcacgttgcc agactgcact tcaagaatgc
     3961 cgccagacag atagataaac tcttcgtgac cgtgctgttt cacgatgcga atcataccag
     4021 gcttaatggc ggtgagcagc ggtgcgtggc cagggtagat ccccagttca ccttcgctac
     4081 ccgttacctg gattttctcg accagaccag agaacatttg ttgctctgcg ctgacgacgt
     4141 ccaggtggta agtcattgcc atatcaccct ccgattaagg cgttaaagtt ttttggcttt
     4201 ttccacagct tcttcgatgg aaccgaccat gtagaacgcc tgctccggca ggtgatcgta
     4261 ttcgccttcc atgatgcctt taaagccacg gatggtgtct ttcagggaga cgtatttacc
     4321 cggagaaccg gtgaatactt ctgccacgaa gaacggctgg gacaggaagc gctggatctt
     4381 acgagcacgc gctaccacca gtttgtcttc ttcagacagt tcatccatac ccaggatggc
     4441 gatgatgtct ttcagttcct gataacgttg caggatggac tgaacgccac gcgcggtgtc
     4501 gtagtgttcc tgaccaacca ccagcgggtc cagctgacgg ctggtggagt ccagcgggtc
     4561 aacggccggg tagataccca gagacgcgat ctgacggctc agtaccacgg ttgcgtcaag
     4621 gtgcgcaaag gtggttgccg gagacgggtc agtcaagtca tccgcaggta cgtataccgc
     4681 ctgtacggag gtgatagaac cagttttggt ggaggtgata cgttcctgca gaacgcccat
     4741 ctcttccgcc agggtcggct gataacctac cgctgaaggc atacggccca gcagtgcgga
     4801 tacttccgta ccggccaggg tgtaacgata gatgttgtca acgaacagca gaacgtcacg
     4861 accttcgtca cggaatttct cagccatggt cagaccggtc agagcaacgc gcagacggtt
     4921 tcccggcggc tcgttcatct ggccatacac cagggatact ttgtcgataa cgttggagtc
     4981 ggtcatttcg tggtagaagt cgttaccctc acgagtacgt tcacctacgc ccgcaaacac
     5041 agagtaaccg gagtgctcga tcgcgatgtt acgaatgagc tccatcatgt ttacggtttt
     5101 acctacaccc gcaccaccga acagaccaac tttaccgccc ttagcgaacg gacacatcag
     5161 gtcgataact ttgataccgg tttccagcag ttcctgagag tttgacagct cttcgtagga
     5221 aggtgctgcg cggtgaatcg cccaacgctc ttcttcaccg atctcgcctt tcatgtcgac
     5281 cggttcaccc agtacgttca tgatacggcc cagagtcgct ttacctaccg ggacttcgat
     5341 cgggtgttcg aggtctttta catccagacc gcgacgcaga ccgtcggagg aacccattgc
     5401 gatggtacgc acgataccgc cgccgagctg ctgctgaact tccagcacca gacgctcatt
     5461 accattttgc acctcaagag catcgtacac gcgcggtacg gcatcctgag ggaattcgac
     5521 gtcaactacg gcgccgatta cctggacaat ctttccagta gccatcttaa atcctctacg
     5581 aaataacctg tttaaaccgc ggcggccccc gagacgatct cggtgagttc ctgagtaatg
     5641 ctggcctgac gagctttgtt gtataccaac tgcagctctt taatcaggct gccgccattg
     5701 tcggtcgcgg ctttcatcgc caccatacgg gcggcctgct cgctggccag gttttcaacc
     5761 acgccctgat aaacctgaga ttcgacataa cgacgcagca gggtatccag caacgccttc
     5821 ggatcgggtt cgtacaggta atcccaggat ttatgtttca gatcatcatc atctgatgcc
     5881 ggtaacggca gcagctggct gatggtcgga acctgagaca tggtgttaat aaatttgttg
     5941 ctgacaatgt aaagcttgtc cagacggcct tcgtcgtagg cctgcaacat cacttttacc
     6001 ggaccgatca gttcggacag ggaagggtta tcccccatgc cggtgacctg ggcaacaaca
     6061 ttgccgccca cggagttgaa gaacgacacg cctttcgagc cgatcattgc gaggtcgcat
     6121 tgaacgcctt tgtcggtcca ggtcttcatt tccgccagca gttttttgaa caggttaatg
     6181 ttcaaaccac cgcacaaacc acggtcggtc gacaccacca ggtagcccac gcgtttaacg
     6241 tcgcggtctt ccaggtaaag gtgcttatat tccagattac catgtgcaag gtgaccaatc
     6301 actttgcgca tggtttctgc ataaggacgg ctggccgcca tgcgatcctg cgatttacgc
     6361 attttggaag cggcgaccat ctccatcgct ttagtgatct tttgcgtgtt ctggacgctt
     6421 gcgatcttac tacgtatctc ttttgcgccg gccatgagct tctcctcaat gccttgcggc
     6481 ctgccctaag gcaagccgcc agacgttacc aggattgggt tgctttgaag gaatcgagga
     6541 tgcctttcag tttgccttcg atttcgtcgt tgtagccacc ggtctggttg atctcttgca
     6601 tcaacggagc gtgatcacgg tcgacgtaag ccagcagagc ggcttcgaag ctgccaattt
     6661 tcgacagttc aacatccgcc aggtaaccac gttctgctgc gaacagaacc agagactgct
     6721 gcgcaacgga catcggcgca tactgtttct gtttcagcag ttcggtcact ttctgaccgt
     6781 ggtcaagctg cttacgtgtt gcatcgtcaa ggtcggatgc aaactgagag aacgctgcca
     6841 gttcacgata ctgtgccaga gcggtacgga taccaccgga cagttttttc atgatcttgg
     6901 tctgtgctgc accaccaaca cgggatacgg aaatacccgg gttaaccgca ggacgaatac
     6961 cggcgttgaa caggttggtt tccaggaaga tctgaccatc ggtaatggag attacgttgg
     7021 tcggaacgaa cgcagaaacg tcacccgcct gagtttcgat aatcggcagt gcggtcagag
     7081 aaccggtttt ccctttcact tcacctttgg tgaaggcttc aacgtattcg gcgttaacac
     7141 gtgcagcacg ctccagcaga cgagagtgga ggtagaaaac gtcgcccggg aatgcttcac
     7201 gtcctggcgg acgacggagc agcagggaga tctgacggta agcaacagcc tgtttagaca
     7261 ggtcatcgta aatgatcagc gcatcttcac cgcggtcacg gaagtattcg cccattgcgc
     7321 aaccggcata cggtgccagg tattgcagtg cagcggattc agacgcggtt gctaccacaa
     7381 cgatggtgtt agccagtgcg ccgtgctctt ccagtttacg taccacgtta gaaatggtgg
     7441 acgctttctg gccgatagcg acatagatac atttgatacc ggaatcgcgc tggttgatga
     7501 tggcatcgat agccagtgcg gttttacctg tctgacggtc accgatgatc aattcacgct
     7561 gaccacgacc gattgggatc atggagtcaa cggctttata accggtctgt accggctgat
     7621 ctacggactg acgttcgata acgcccggag cgattgcttc tacagcagag aagccgtcgt
     7681 gatccagcgg acctttaccg tcgattggtg cacccagagt gttaaccaca cggcccagca
     7741 ggccacggcc aaccggaact tccaggatac ggccagtaca cttaactttc atgccttcgg
     7801 caaggtcagc gtacggaccc ataacaaccg cacctacaga gtcgcgctcg aggttcagtg
     7861 cgatagcgta acggttaccc ggcagggaga tcatttcacc ctgcatacaa tcggccaggc
     7921 cgtgaatgcg gataacaccg tcacttacag aaacaatagt accttcgttg tgagcttcac
     7981 tcacaacatt gaactgagca atgcgctgct tgatcagttc gctgatttcg gtggaattca
     8041 gttgcatgct ccagtcccct taagactgca agacgtctgc aaggcgctca agacgaccgc
     8101 gtacgctgcc atcaatgacc atatcacccg ctcggatgat aacgcctgcc attacagact
     8161 tatcgatttt gcaattcagc ttaactttgc gtgacagacg tttttccatc gcagcagaaa
     8221 ttttcgcgag ctgttgttca ctcagtgcgg cagcggaaat gacgtctacc tcagcggtag
     8281 cctcactcac ggcacgaagg tgaataaact gctccagaac atccgggagc gcgttaagac
     8341 gaccattttc agccattacc cgaatcaggt tctgaccgtt ttcgtccagt tgctcaccac
     8401 aaactgcgat aaacgactcg gcgagcgttt ctggcgcaag cgcgccagag agaagctctg
     8461 ccatttgttc gtttttggtt acctcggcgg caaacgccag catgtcctgc cagcgttcta
     8521 cactttggtg ttcgacggca aagtcaaaag ctgctttggc gtaggggcga gctaccgtaa
     8581 taaattcaga catcagcccc tccctcctta cagttcagcg acaagtttat ccacgatgtc
     8641 gctgttagca gcttcatcca cggaacgttc gatgatcttc tcggcgccag caacagccag
     8701 gatagcaact tgcttacgca gctcttcacg ggcacgttta cgctcggctt caatttctgc
     8761 ctgcgcctgg gccacgattt tagtacgttc ctgttctgcc tcagctttcg cttcgtccag
     8821 aatctgcgag cggcgtttgt tcgcctgctc gatgattacc tgggcttccg ctttcgcttt
     8881 tttcagctgg tcggtcgcgc tggcctttgc aaggtcaagg tccttatgtg ctcgttctgc
     8941 ggaagcaagg ccgtcagcaa tttctttttg acgtttttcg atggctgcca ttaatggcgg
     9001 ccatacgtac ttcatgcaga acagaacgaa caggacaaac gcgatggcct ggccgaggat
     9061 tgttgcgtta agattcacag cacaatgcct ctatttagtt aacgttctga tattgctctt
     9121 taaataaaag caacgcttac tacgcgacag cgaacatcac gtacagaccc agacctacag
     9181 cgatcatcgg gatagcatcc accagaccca taacgataaa gaactgagta cgcagcagag
     9241 gaatcagatc aggttgacgc gctgcgcctt ccaagaattt acccccgagg atgccgatac
     9301 cgatcgcagc accgattgcc gccagaccca tcatcacagc ggcagccatg tacagcagat
     9361 ccatattcag gttttccatg acagtctcca gtttgtttca gttaaaacgt agtagtgttg
     9421 gtaaattaat gttcttcaga cgccatcgac agatagacga tcgtcagaac catgaagatg
     9481 aaggcttgca gcgtaatgat caggatgtgg aaaatggccc acggcacatt caggatccac
     9541 tgtgaccacc acggcaacag accagcaatc agaatgaaaa tcagctcacc ggcatacatg
     9601 ttaccgaaca gtcgcaaacc gagtgaaact ggtttggaca gcaggcttac cccttcaagg
     9661 attaagttga caggaatgaa cgcccagtga ttgaacggct gcagcgtcaa ctctttcgtg
     9721 aagccgccga tgcctttcat tttgatgctg tagaacagaa tcaggataaa tacgcccaat
     9781 gccatagaca gcgttacgtt cacgtccgca gacggaacca cacgcagtgc aggcagaccc
     9841 agtacatgtt cagcaatgta cggcagcagg tcgataggca gtaaatccat caggttcatc
     9901 aggaataccc agacgaagat cgtcagggcc agcggagcaa tcagcttgct tttgccatgg
     9961 tacatgtctt tcacgctacc attaacaaag ccgatcacca gctcaatcgc ggtctgaaac
    10021 ttacctggca caccgctggt cgcctttttg gctacgctac ggaataaaac caggaacaac
    10081 agacccagca ccaccgagaa gaacatggag tcaatattga ttgtccagaa ggtggctggg
    10141 gggttttgtg gatccaccag cgagaatgta cgcaggtcca gctgaaggtt attcaggtgg
    10201 tgtcctatgt aatcctgcgg cgtcatattt tctgaagcca tgatgccttt taccctttgt
    10261 tgttaattac agccggtgcc agtatctgaa ccaccagcac caaaacccac gtaacgatca
    10321 gcggcaagaa taccgccttt aaaaccgcca acgccaccac cagtaacacc aacatcgcca
    10381 gaactttgaa agcttcgcca aatgcgaatg tccaggccac ccggcctttc gctggtgtat
    10441 gcgcctggtg acgccaagca aatatcataa acaaaacgtt aggcagaaag actgccaggc
    10501 ccccgcttat tgcagagacg ccccagaagg ggtctttgag gctgaacagc aatccacttg
    10561 ctatcaccac cagtaactga acgagcagaa gcttccgagc aacgtttcga ctcacgagcg
    10621 acacagacat cacgtttttt actcctgctc ccttcgaggt atgccgcgtg tcgtataaaa
    10681 ctttctttaa ggcttagagt caagcatcaa aaagcggtca aattatacgg cgcgcccccg
    10741 tgatttcaaa caataagtag ccaaaaggtg aataaatgtt taaatatttt tccagtgcat
    10801 acaattgcga cttttctact aaccctgttc gatcatgaaa aactgtaaat aacgcgtaaa
    10861 cactggtgat aaagcgtgct tcagatcaca tattgcgcat gttcgcgcac agcatattta
    10921 tttacttggc aaatgatgcc tttgcaagtt tatgatattt cagtctaaaa acagatactg
    10981 ttttaataaa tgacatttac acaacaaaaa ccacccattg acatttttaa taatgtttta
    11041 gcagccaatg atggttctta gcgccgattt ttagcagact gatattttca ctaatgactt
    11101 attttctgct taccaaaaaa agccacgtta tcttgttgat gcaaaagagt gaacgtggcg
    11161 ttaaatgtaa ccagttatat cagtagaaaa cctggttgtt gttaacagtc taaccggtca
    11221 attttttatg atttttttga taaaaattaa attttatttg ctttaatcac caccagatga
    11281 cgttcgccat ccagggctgg aacctgaagt ttaaccactg attcgacctg atattcttcg
    11341 ggcaacaaag cgatttcatc ttccggcatt tgccctttca gcgcgtagaa acggccttgc
    11401 tcaccaggaa gatggtggca ccagctcacc atatcgttca gagaggcaaa agcgcggcta
    11461 attacgccat caaatggcgg ctctgaagga aactcttcta ccctgctctg tacaggttca
    11521 atattctcca gtttaagctc atgttgcacc tgacgaagga aacgcacgcg tttaccaagg
    11581 ctatccaaca gagtgaaatg ggcttcagga cgcacgatag agagtggaat gcctggcagt
    11641 cctggtccgg tgccgacatc gataaaccgt tcaccttgca gatacggtgc caccacaatg
    11701 ctatcgagaa tatggcgtac cagcatctca ttaggatcgc ggaccgaagt caggttgtac
    11761 gctttgttcc atttatgcag catattcacg taggcaataa gctggttttt ctggtgatcg
    11821 gtaagcgaaa tacctgcgtc tttcagcagt aaggagagtt tgttgagcac ggtgattacc
    11881 tgttcttgat gcgttgcctg gtaagcgggt gcttaccagg catttttaat gcgttatgcg
    11941 ctacgacgca gcataccctg ttttttcagc cacaccagca gaatggagat agccgcaggc
    12001 gtgacgccag aaatacgcga agcttggccg atagaggctg gtttgtgatc gttaagtttg
    12061 gcgatcactt cgttagaaag accggatacc tggcggtaat ccagtgtcgc gggtagcagg
    12121 gtgttctcgt tacgcagctg cttttcgatc tcatcttgct ggcgcgcgat ataaccttcg
    12181 tatttaacct gaatctcaac ctgttccgcc gcctgttcgt ctgtcaacgc aggggcaaac
    12241 ggcgtcagcg tggttaattt ttcataagtc atttccggac gacgcagcag atcttcacca
    12301 ctggcttcac gggaaagcgg cgcagtcagg tgagcattca cttcggctgc agcttccgcc
    12361 gacggggtta cccaggtcga tttcagacgc tgacgctcac gctcgatatt ctcaagtttc
    12421 tcgttaaagc gcgcccaacg ttcgtcatcc accaggccca gttcacgacc gatttcagtc
    12481 aaacgcagat ccgcattatc ttcgcgtagc atcagacgat attctgcgcg cgaagtaaac
    12541 atacgatacg gttctttggt tcctaaagtg cacaggtcat caactagtac gccgagatac
    12601 gcctgagaac gtgccggagc ccaaccttct ttgtcagcag acagacgggc agcgttaaga
    12661 ccggccagca aaccttgcgc agcggcttct tcgtaaccgg tagtgccgtt aatctgacca
    12721 gcaaagaaca gcccctggat aaacttgctc tccagcgtcg gtttcaggtc gcgaggatcg
    12781 aagaagtcat actcaatggc ataacccgga cgcacgatct tcgcgttttc catcccctgc
    12841 atagagcgga cgatttgcat ctgcacatcg aacggcaggc tggtggagat accgttcgga
    12901 taaatttcat tagaggtcag tccttccggt tcaaggaaga tctgatgctg atttctgtcg
    12961 gcgaagcgca tgactttgtc ttcgatcgac gggcagtagc gtgggccgac accttcgatc
    13021 acccctgcat acattgggct acgatcgagg ttactgcgga tcacatcatg ggttttctcg
    13081 ttggtatgag tgatataaca cggcacctgc tggggatgct gggacgcatt gcccataaac
    13141 gagaataccg gcattgggtt atcgccatgc tgttgcgcca gcacactaaa gtcgatagtt
    13201 cgagcatcaa tacgcggtgg tgtcccggtt ttcagacgac caacgcgcag cggcagttca
    13261 cgcaaacggc gagaaagcgg aatggacggc ggatcaccag cacggccacc gctgtaatta
    13321 tccagaccga tatgaatttt accgtcgagg aacgtcccaa cggtgagcac gacggctttg
    13381 gcacggaact tcagtcccat ttgggtaaca gcaccgacca cgcgatcgtt ttcgacaata
    13441 agatcttcaa ccgcctgctg gaagatcatc aggttcggtt ggttctccag cgccgtacgt
    13501 accgcctgac ggtagagcac acgatccgcc tgagctcggg tagcgcgaac cgccggtcct
    13561 ttgcttgcgt ttagtatcct aaactggata cccgcttgat cgatcgcttt cgccatcaga
    13621 ccgccgagtg catccacttc ttttaccaga tgtcccttcc caataccgcc gatcgccggg
    13681 ttgcagctca tctgccccag agtgtcgata ttgtgtgtca aaagcagagt ctgttgaccc
    13741 atacgcgccg cggccatcgc ggcctcggtg cctgcatgac ccccgccaat gatgatgacg
    13801 tcaaaaggat ccggataaaa catggtgatt gcctcgcata acgcggtatg aaaatggatt
    13861 gaagcccggg ccgtggattc tactcaactt tgtcggcttg agaaagacct gggatcctgg
    13921 gtattaaaaa ggagatctat ttatttagag atctgttcta ttgtgatctc ttattaggat
    13981 cgcactgccc tgtggataac aaggatccgg cttttaagat caacaacctg gaaaggatca
    14041 ttaactgtga atgatcggtg atcctggacc gtataagctg ggatcagaat gaggggttat
    14101 acacaactca aaaactgaac aaccgttgtt ccttggataa ctaccggttg aaccaagctt
    14161 cctgacagag ttatccacag tagaacgcac gatctgtata cttatttgag taaattaatc
    14221 cacgatccca accattcttc tgccggatct tccggaatgt cgtgatcaag aatgttgatc
    14281 ttcagtgttt cgcctgtctg ttttgcaccg gaatttttga gttctgcctc gagtttatcg
    14341 atagccccac aaaaggtgtc atattcacga ctaccaatac cgattgcgcc aaagcggacc
    14401 gcagaaagat cgggcttctg ttcctgcaat gcttcataga aaggagaaag gttgtccgga
    14461 atatctccgg caccgtgggt ggagctgata atcagccaga tccccgaggc agataaatct
    14521 tccaacagcg gaccgtgcag cgtttcggtg gtaaaacccg cctcttccag cttttcagcc
    14581 aggtgttctg ctacatattc ggcaccgccg agggtgctgc cgctgataag agtgatatct
    14641 gccataaacc gccaccttta ttaagagtgg cgtattgtac gctgtgaacg cgttgggatc
    14701 tacctgtgga aaagtatggg attaaaaaag ccgatcaggg cttgatagta cgcatgatcg
    14761 ggtgttgcag gacgatcaat gtctcggtgg actgaatttc atcaattgtt tggatcttgt
    14821 tgataagtac atgctggaga gcgtcgatcg aacggcacat tacttttata aagatgctgt
    14881 agtggcctgt tgtgtaatag gcttcagtga cttcatcaag gctttccagc tttgccagcg
    14941 cggaagggta gtctttggcg ctctttaata taatgccgat aaagcagcct acgtcataac
    15001 cgagctgctt cgggctgaca tcaatacgcg ccccggtaat gatccccgcc tgcttcattt
    15061 tctctacccg aacgtgaatc gtccccggac tgacgccaaa ttgtttcgcc agttcggcgt
    15121 aagcggtgcg cgcattgccc attaatgctt ccaggatgcc acggtccaga ttgtcgatca
    15181 gataattttc cataggattt tcttatgcag attgatgatt cattctattt tagccttctt
    15241 ttttaatgaa tcaaaagtgt gttaggcttt ttattgaatg attattgcat gtgtgtcggt
    15301 ttttgttgct taatcataag caacaggacg caggagtata aaaaatgaaa accgcttaca
    15361 ttgccaaaca acgccaaatt agcttcgtga aatctcactt ttctcgtcaa ctggaagaac
    15421 gtctggggct gatcgaagtc caggcaccga ttcttagccg tgtgggggat ggcacgcagg
    15481 ataacttgtc gggctgtgaa aaagcggtgc aggtaaaagt gaaagctctg cctgatgccc
    15541 agttcgaagt ggttcattca ctggcgaagt ggaaacgtca gaccttaggg caacacgact
    15601 tcagcgcggg cgaagggctg tacacgcaca tgaaagccct tcgccccgat gaagaccgtc
    15661 tttctccgtt gcactcggtc tatgtagacc agtgggactg ggaacgcgta atgggcgacg
    15721 gtgagcgtca attctcgact ctgaaaagca cggtagaggc gatctgggcg ggaattaaag
    15781 caaccgaagc tgcggttagc gaagagtttg gcctggcacc gttcctgccg gatcagatcc
    15841 acttcgtaca cagccaggag ttactgtctc gttatctgga tctcgatgcc aaagggcgtg
    15901 agcgggcgat agcgaaagat cttggcgcgg tattccttgt cgggattggc ggcaagctga
    15961 gcgatggtca tcgccacgac gtgcgcgcac cggattatga tgactggagc accccgtcag
    16021 agctgggtca tgcgggtctg aacggcgata ttctggtgtg gaaccctgta ctggaagatg
    16081 cgtttgagct ttcttccatg gggatccgcg tggatgccga cacgctgaag catcagctgg
    16141 cgctgaccgg tgatgaagat cgtctgcagc tggaatggca tcaggcgctg ctgcgcggtg
    16201 aaatgccgca gaccatcggc ggcggtatcg gccagtctcg tttgactatg ctgctgctgc
    16261 aactgccgca tatcggccag gttcagtgtg gagtatggcc agctgctgtt cgcgagagcg
    16321 tcccttctct gctgtaataa tttatcgccg ccagcgtctg agcaggcggc ttcgcatccc
    16381 ggtatcaaag cgccagatat gatcgaaaat gcgcatgatg ccgggtttgc cgtgtgccga
    16441 catcgccacg gcatgaaagc gatgctgatg tacccgctgc agctctttca ctttactcgt
    16501 cacgtcgtca ggcaaccgct gagcgataaa atcagaaatc accaccgcat cggcatcaaa
    16561 ccattccctg ctttgcaagc gttccataat ggcgcgaaaa caactggcaa gatcggtgcc
    16621 gccacgaaac tgctggctta aaaaacggat tgcttgttcg atgccttgtg ggcctgaaag
    16681 ctcataacgg acgatctcgg tggaaaatag cataatatag cagcgccggt tttctgcgag
    16741 agcaatgcgc atcaaggcca ggcagaacgc tttcgcacac tgttcattaa agccgcccat
    16801 tgagccggaa gtatccacac agacaataaa cggcccgcgc ggctgttcat cgtaatcttt
    16861 atgtaccacc ggacgttcga tcactttttc acgccacgac tcaccgtgca ggcgataggt
    16921 gagcaactgt ttttccacca gccgacggta aaactcatac tccagttccg ttatccctag
    16981 tgtcgccagt tctggcggca ggagacgtaa aatatcatcg ctttgttgca gaccatcaac
    17041 ctgctcagga accgtcgccg gttcgcgcac catggtgcgg aaggtttcca tctgcgcatc
    17101 gttgcgcggt attgatttgg cttcccgaga acgccccagc tgctctgcca ggcgtttcag
    17161 ttccggctgt tcgttaagaa attcaccgta tttcacaatc aactgatagt cgccacgttt
    17221 aagctggccg gcgctcatat cccacagacg accagctgcg gtattgttat ctgcgagaat
    17281 cggttcaagt tgtccgctca gcgtcatgcg ttcctgaact tcacttaaca gttgttcgcg
    17341 ttcttcttct aatagctgtt gatttaacgt cgttgcttgc acgatcagac ttaaacgcca
    17401 gcgctggaga aaaagcgtgt gtaacgctga agttattgtg ctgttagcat caaccaactg
    17461 ccgggcttgt tctgcccatg gggaattcag acgatgcagt aagtccagga tctgtggtag
    17521 ctgcacaata aactgcggcg tggagaggag ctggctttgc tgatagcaca tcacctcttc
    17581 ggtgagttct ggcgggactc gggcatcttt cagccgactg cgcagcgcct cacgccagcg
    17641 gggaacatca tcagtgattg ctgccttcag tcgtgggaat ttttcaaaga agaccgccag
    17701 ctgcggtgag gccagcagtg cgatgatcat ctcttcgatc aatccctctt cgctgacggc
    17761 cagcatcaca ttaagcgtat ccagcgttag cattgttgtg cctggcgaat ctgtgcaccg
    17821 acatcctgta ggctggcttc aatgcgacct aaccagtcgc cagggataaa cagacatttt
    17881 tgctgttcgc tgaataaagc gtgttgctta cgccagtcgc tttcaagctc ttccagttgt
    17941 tgttttattt cacctggcag accttcagcc gatgaaccgg gaagtgccag cgtactgcct
    18001 tgtaaactta catcgcgtac aacaagatgt tgggtgctat caacttccag attcagtttc
    18061 tgggcaaagc cgataccgtt cagtttgccg cgaatttcac cacctttact cagccactgt
    18121 tccagcgcgc tacgctcaaa ggagatatga accacttcca tatcatgcag ttttaacggt
    18181 ttttgcagca gcagagtcag agtggaagca gtaacgttaa cagggagttg atactgctga
    18241 cgacggctaa aaatgccgcc cagacgaatt accgttaagg ctgttttatc gctttgttgc
    18301 tgctgaagtt gcaggtgacg ttgcacaatc gcgcctaggc gggtcaacat cccttgctgt
    18361 tgccaggcgt gaccggtcat caatacatca atttgttgtt gtatcaaatt caggctttgc
    18421 gcgtcatacc acaggcaatc tttcagcaaa atgagatcta ccggggcaac agcactgcga
    18481 ccgctaaaaa aggcgctggc ctgcaataat cggatcgctt ttttccagcg acgatccgag
    18541 acataaggcg catccggtaa tttatccagt tgctggcgca gcataaaaat cagctcaaat
    18601 acatgatcgg gcagcgtaat ttcaccaatc tctttctgcc agcgttcata ttcttcatct
    18661 gtgacctgca aggcatcagg aaccggattg tcgttttcat cctgttgact ggtcagcatg
    18721 gagcggaaat tcgctttatc ctgcacttta tctaaccaca gacgaatcag catgcggtca
    18781 tataacgctt ccagactgct gtctgcttcc ggcagctcgt tggaggccgc caccagcaga
    18841 cgcatcggga ttttttctac gtgtgcaccg ttgcggaact ggcgctcgtt aatggcggtg
    18901 agcaaggtat taagaattgc cgggcccgct ttccagatct catccagaaa gacaatttct
    18961 acttccggca ggtaaccgct ggttaaacgt tcatagcgcc cttcatcttt tagcgcctga
    19021 atagaaaggg gaccaaaaac ttcttccggc gtggagaagc gggtcatcag atattcaaac
    19081 gcgcgggcat tctgaaaggc gaattttaag cgccgggcga tcaaactttt ggcaatacct
    19141 ggcgggccaa ggaggaacac actttcacca cttaatgccg ctaataaaca caagcggatg
    19201 gcgtggctac gttcataaag ccccttttcc aacgaactgc tcaggcggga aattctttcc
    19261 gctaataaat gagggtgagc cataatgaag tggcgtcctt tcgtcaaaag ttctgcgtaa
    19321 attgcgagta tagacgtttc ttgctggtgg ctaaaatagt ctcaaag
//
LOCUS       DADLFA010000068        19309 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000068 DADLFA010000000
VERSION     DADLFA010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19309
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.068"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..731
                     /gene="rihB"
                     /locus_tag="JQH65_002023"
     CDS             <1..731
                     /gene="rihB"
                     /locus_tag="JQH65_002023"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HAY6139442.1"
                     /translation="MRKQIVADNIHGETGLDGPVFESLTRQAESTHAVKYIIDTLMAS
                     DGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFTPSAEFNIFADPEA
                     ARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSDIMNFTLKTQFENY
                     GLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCDELGVLGKPANTKV
                     GITIDTDWFWGLVEECVRGYIKTH"
     gene            831..2081
                     /locus_tag="JQH65_002024"
     CDS             831..2081
                     /locus_tag="JQH65_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAY6139443.1"
                     /translation="MDVMRSVLGMVVLLTIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPAGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDHLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFAHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRVPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(2137..3225)
                     /locus_tag="JQH65_002025"
     CDS             complement(2137..3225)
                     /locus_tag="JQH65_002025"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAY6139444.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGIADIRYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGAGDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(3228..4085)
                     /gene="nfo"
                     /locus_tag="JQH65_002026"
     CDS             complement(3228..4085)
                     /gene="nfo"
                     /locus_tag="JQH65_002026"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAY6139445.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(4159..5208)
                     /locus_tag="JQH65_002027"
     CDS             complement(4159..5208)
                     /locus_tag="JQH65_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HAY6139446.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            5308..6189
                     /locus_tag="JQH65_002028"
     CDS             5308..6189
                     /locus_tag="JQH65_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY6139447.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANIPR"
     gene            6394..7863
                     /gene="lysP"
                     /locus_tag="JQH65_002029"
     CDS             6394..7863
                     /gene="lysP"
                     /locus_tag="JQH65_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HAY6139448.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            8156..10136
                     /gene="cirA"
                     /locus_tag="JQH65_002030"
                     /pseudo
     CDS             8156..10136
                     /gene="cirA"
                     /locus_tag="JQH65_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(10168..11004)
                     /gene="yeiG"
                     /locus_tag="JQH65_002031"
     CDS             complement(10168..11004)
                     /gene="yeiG"
                     /locus_tag="JQH65_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HAY6139449.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            11262..11930
                     /gene="folE"
                     /locus_tag="JQH65_002032"
     CDS             11262..11930
                     /gene="folE"
                     /locus_tag="JQH65_002032"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HAY6139450.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            11947..13104
                     /locus_tag="JQH65_002033"
     CDS             11947..13104
                     /locus_tag="JQH65_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HAY6139451.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            13246..14286
                     /gene="galS"
                     /locus_tag="JQH65_002034"
     CDS             13246..14286
                     /gene="galS"
                     /locus_tag="JQH65_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HAY6139452.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAIFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTMRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            14566..15564
                     /gene="mglB"
                     /locus_tag="JQH65_002035"
     CDS             14566..15564
                     /gene="mglB"
                     /locus_tag="JQH65_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HAY6139453.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQSVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            15625..17145
                     /gene="mglA"
                     /locus_tag="JQH65_002036"
     CDS             15625..17145
                     /gene="mglA"
                     /locus_tag="JQH65_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HAY6139454.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLRRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDKSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            17161..18171
                     /gene="mglC"
                     /locus_tag="JQH65_002037"
     CDS             17161..18171
                     /gene="mglC"
                     /locus_tag="JQH65_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HAY6139455.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPCWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(18414..19309)
                     /gene="preA"
                     /locus_tag="JQH65_002038"
                     /pseudo
     CDS             complement(18414..19309)
                     /gene="preA"
                     /locus_tag="JQH65_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
BASE COUNT         4600 a         4773 c         5118 g         4818 t
ORIGIN      
        1 ttatgcgtaa acaaatcgtt gccgataata ttcacggtga aaccggactg gatggtccgg
       61 tattcgagtc gctgacccgc caggcagaaa gtacccacgc ggtgaaatac atcattgata
      121 ccctgatggc aagcgatggc gatatcactc tggtgccggt tggtccgctt tcaaatatcg
      181 cggtggcaat gcgtatgcaa cccgcgatcc tgcccaaaat ccgtgaaatt gtgctaatgg
      241 gcggcgctta cggtacaggc aacttcacgc catctgccga gttcaacatc tttgctgatc
      301 cggaagcagc ccgcgtggtg ttcacctccg gcgttccatt agtgatgatg ggcctcgatc
      361 tcaccaacca gactgtttgc accccggacg tgattgcacg gatggaaagg gcaggcggcc
      421 ctgccggaga gctgttcagc gacatcatga acttcactct caaaacgcag ttcgaaaact
      481 acggcctggc tggcggcccg gtgcacgacg ccacctgcat cggttatctg attaaccctg
      541 atggcattaa aacccaggag atgtacgtcg aagtggacgt caacagtggc ccttgctatg
      601 ggcgtaccgt ctgcgacgag ctgggcgttc ttggcaagcc cgctaatacc aaagtcggca
      661 tcactattga taccgactgg ttctggggct tagtcgaaga gtgcgtgcgc ggctacatca
      721 aaacccatta agtgttgata acaagccggg tcagtatcgc aagatccaag cccggctatt
      781 tctgatgggc agaaagccgt cgcccaatat gacatgaaga gataataact atggatgtca
      841 tgagaagtgt tctgggaatg gtggtattgc tgacgattgc gtttttgctg tcagtaaaca
      901 agaagaagat cagcctgcgt accgttggcg cggcgttagt gttacaggtc gtgattggcg
      961 ggattatgct ttggttaccg gcagggcgtt gggtcgctga aaaagtcgct tttggcgtgc
     1021 ataaagtgat ggcgtacagc gacgcgggta gcgcatttat cttcggttcg ctggtcgggc
     1081 cgaaaatgga cacgctgttt gatggcgcag gatttatctt tggtttcagg gtattaccgg
     1141 caattatctt cgtcactgca ctggtgagta ttctctacta catcggtgtg atggggattt
     1201 taattcgcat tctcggcggt atattccaga aagcattaaa tatcagcaag attgagtcat
     1261 tcgtcgcggt caccaccatt ttcctcgggc aaaacgaaat tccggcgatc gtcaaaccct
     1321 ttatcgatca tctgaatcgc aatgaattat ttacagcgat ttgtagtggc atggcctcga
     1381 ttgctggttc gacaatgatt ggttacgccg ccctgggcgt acctgtggaa tatttgctgg
     1441 cggcatcgtt aatggcgatc ccaggcggga tcttgtttgc tcgcctgtta agcccggcta
     1501 cggaatcttc gcaggtttcc tttaataacc tctctttcac cgaaacaccg ccaaaaagca
     1561 ttattgaagc cgctgcgaca ggggcaatga ccgggctgaa aatcgccgcc ggtgtagcga
     1621 cagttgttat ggcatttgtc gccatcattg cgttaattaa tggtattatc ggcggcgttg
     1681 gcggctggtt tggttttgca catgcctcgc tggagtccat tttaggttac ctgttggccc
     1741 cattggcgtg ggtgatgggg gttgactgga gtgatgcaaa tcttgccggg agtttgattg
     1801 ggcagaagct ggcgatcaat gaatttgtcg cttatctcaa tttctcgcca tatctgcaaa
     1861 cgggtggcac tctggatgct aaaaccgtgg cgattatttc tttcgcgttg tgcggtttcg
     1921 ctaactttgg ttctatcggg gtggtggtgg gggcgttttc tgcggttgcg ccacaccgtg
     1981 tgccggaaat cgcccaactt ggtttacgcg cgctggcggc ggcgacactt tctaacctga
     2041 tgagtgctac tattgcagga ttctttattg gtttagcgta gtgcgcaatt tatgagccgg
     2101 ataagatgcg gtcaagcgtc gaatccggca acattgttac accattggca ctaatgaaag
     2161 cgcgttatcg gcagacaggg tagggttgtt taagctgccg ctggcgcgcg aaattgccgc
     2221 gcaggctacc gcaaaacggg cgctgtcgcg gaaactgtat ccttcaagaa agctatatac
     2281 caggcccgcc ataaaaccat cgccagcacc aaaactgtct actgtcgtat gcgctggcgc
     2341 ggtcagcaaa aattgttctc catccttttc gctgcaataa actgactcat cgggcaaata
     2401 aacaaacagt tgctgaacac cttgctgatg caatgcattc actgcggcat tacggtcagc
     2461 atcgctggtg atcgcctgtc cccataaaat ttccagctcc ggtaaagtgg gtttcagggt
     2521 gtgaatatgc gccagccagt gtttgatttt gcccgctttg aattctgaaa cggtatcgac
     2581 aaacaccggg atttcatcag cgagggtaaa gacccattcc agcgcctcgg ctgtcaggtt
     2641 gcaatcagcc agtaccacgc ccgcatgacg aagtaaatcg cgcgacccgt tcagtagttg
     2701 cggtgtcaac tgttccagca gatgggtatc gttaatcgcc agcacggttt gatcgtctcg
     2761 attggcaatt gccagatacg tcgatgtgct ttgaccatgc aaacgaacgc agccggagac
     2821 attcacgccg gcacggcgcg tttcttccag gagcatttcg ccataaaagt catcgccaat
     2881 cactgaaagc aaatggacgt cacggcctaa cagcgccaga ttgtgggcga tgttgcgtcc
     2941 cacgccgccc gccgagcaat gaattgtacc gggatgagaa gccgcttgcg ggtaacggat
     3001 atccgcgatc ccgcgaatat ccatattgat tgtccccacc actacgcagt attcctgctc
     3061 ggtgagaatg taacctttgc ctttaatccg ccctttgcgc attaaatcca taatatgcgc
     3121 cgcaacgcgc gaacgactga tttgcagcat gtccgcaatt tcgttctgct gaatcagcgg
     3181 gttacgccgt aaaattgcaa ggatctcctt ttcccggtta ttcatcttca ggctaccgct
     3241 ttttcagttt gttgcgcttt cagccaggcg atctcttctg cccagatatc cgggttgatg
     3301 gtttcaagga tcagcggaat gccgtcgaaa cggtcgtcct gcatgatcca gcggaacgca
     3361 tcatgaccga tattgccttc accgaggcta tgatggcggt caacgcggct gccaaaggtg
     3421 cttttcgcat cgttaaggtg catcccgcgc agatacttaa agccaacaat acgggcaaaa
     3481 tccgcgaacg ttttctcgca ttcggctgga gtacgcaaat catacccggc agcgaaagca
     3541 tggcaggtat caatgcagac gccgacacgg gatttatctt ccacgccgtc gataatcgcc
     3601 gcgagatgtt cgaatttaaa ccctaagtta ctgccctgac cggcggtgtt ttcaatcacc
     3661 gctgttacac cttgagtttt atccagcgca atgttgatgg attcggcaat acgcgcaagg
     3721 caatcctctt ctgaaatctg catcagatgg ctgccagggt ggaagttgag caaagaaagc
     3781 cccagttgtt cgcaacgctg catttcatct ataaaggcat cgcgcgattt ttccagagct
     3841 tcagtgaccg gatgtccgag gttaatcaga tagctgtcgt ggggaagaat ttgcgccgat
     3901 gtgtagtgat atttttcaca ggctgctttg aattcatcga tggtttgcgt cgtgagcggt
     3961 gcggcacgcc actgacgttg gtttttggtg aacaaggcaa acgcggttgc gtcgatttcg
     4021 gcggcgcgaa ttgcggcatt tgccagaccg ccagcagcac taacgtgcgc tccaatgtat
     4081 ttcatgcgag gactcctgtt aaacccgctg gaggaaaacg gtaatgatag cgggttaaca
     4141 ggagcaagat gtagtggttt atgcgatgac gctttgaatc acatagttaa tcgcaccacc
     4201 accaacaatc agccaggcaa acagtaccag tgccatcagc agaggtttcg ccccggcttt
     4261 tttcagcgcg ctgacgtgag tggtcagacc cagcgccgcc atcgccattg ccagcaggaa
     4321 ggtatccagc gttaccagca tgttcaccac gctctgcggt aacaggtgga acgagttaaa
     4381 gatggcaacg acgatgaaca agatggcaaa ccacggaata gtgattttgc ttttctcgcc
     4441 gctgttcgcc ccagacagct gtttaacacg cgccgccagc aggatgagga acggagccag
     4501 catcatcacg cgcagcattt tggaaataac tgctgcgttt tccgcatccg ggctgatggc
     4561 atgacctgcc gccaccacct gcgccacttc gtgcacagta gaaccaatgt agataccgaa
     4621 agtttccgga ctaaaccatt gagacatcag cggatatatc gccgggtaga ggaaaatcgc
     4681 gacggtcccg aagataacaa cggttgcaac agccacggtt actttactgg cttccgcttt
     4741 cactaccggc tcagtcgcca gtaccgcggc agcaccacag atactgctac cggcaccgat
     4801 caaccagctg gtgtgcttat ccagaccaaa cactttctgc cccaggaagc aagccagcag
     4861 gaaggtactg gacagcgtca acacgtcaat gatgatccca ctgataccga catcggcaat
     4921 ttgcgagaac gtcagacgga agccataaag aatgataccc agacgtaata aatattgctt
     4981 ggcaaacagc acaccaccgt cacagctttt ccagatgtgc ggatagatgg tgttgcctaa
     5041 aaccatcccc aacaagattg cgagggtgag ggcactaaac ccggcacccg caaccgcggg
     5101 aatggaacca ccccacaggg cgaccccggt gataactgca ctcagggcta accccggaat
     5161 aaaatgccac agtgtacgat gttgtttctg taaggtgata ttcgtcataa ccctctcctt
     5221 ataaccgggc ataaggttac gactaactgg tttaaaaata aaattgatta tatatttata
     5281 attaatcttt ataagtggta agcgactatg cacatcaccc tccggcagtt ggaagttttt
     5341 gcagaagtat tgaaaagtgg atcaaccacc caggcgtcgg tgatgctggc gttgtcgcaa
     5401 tcagcagtga gcgcagcctt gaccgacctg gaagggcagc ttggcgtgca actgtttgat
     5461 cgcgtgggga aaagactggt tgttaatgaa cacgggcggc tgctctatcc gcgtgcgttg
     5521 gcattgcttg aacaggcggt tgaaatcgaa caactgtttc gcgaagacaa cggcgcgatt
     5581 cgtatctatg ccagtagtac catcggtaac tacattctgc ctgcagttat cgcccgttat
     5641 cgccatgatt atccgcagtt gccgattgaa cttagcgttg ggaacagcca ggacgtgatg
     5701 caagcggtgc tggatttccg cgttgatatt ggctttattg aaggaccgtg ccacagcact
     5761 gaaatcattt ctgaaccgtg gctggaagac gagctggtgg ttttcgccgc gccgacttcg
     5821 ccgttggctc gtggtccggt caccttagaa cagctggccg ctgcgccgtg gatcctgcgt
     5881 gaacgcggtt ccggcacgcg ggagattgtc gattatctat tgctgtcaca tttaccgaag
     5941 tttgagatgg cgatggaatt aggtaactcc gaggcaatca aacatgcggt gcgtcatggg
     6001 ttgggaatta gttgcctgtc gcgacgggtg attgaagatc aattgcaggc aggcacatta
     6061 agtgaagttg cggtccctct gccgcgcctg atgcgtacgt tgtggcgtat acatcatcgg
     6121 caaaaacacc tttccaacgc gctacggcgc tttctggact attgcgatcc cgcgaatata
     6181 ccgcgttaag ttgctgtaca agaacatgct ggtgctgtgt cgatttcgtg acgcagcgcc
     6241 ctcagcatgc attcgccaga aaagagattg gctgctttac ttataatccc agggcgatca
     6301 tgaaggtgtc ttataaccgt gtatttctgc cggaaggatt gccaatcgtc tgctacaatc
     6361 gcgcctcatt tttaagatgg atagcatttt tgtatggttt ccgaaactaa aaccacagaa
     6421 gcgccgggct tacgccgtga attaaaggcg cgtcacctga cgatgattgc cattggcggt
     6481 tccatcggta caggtctttt tgttgcctct ggcgcaacga tttctcaggc aggtccgggc
     6541 ggggcattgc tctcgtatat gctgattggc ctgatggttt acttcctgat gaccagtctc
     6601 ggtgaactgg ctgcatatat gccggtttcc ggttcgtttg ccacttacgg tcagaactat
     6661 gttgaagaag gctttggctt cgcgctgggc tggaactact ggtacaactg ggcggtgact
     6721 atcgccgttg acctggttgc agctcagctg gtcatgagct ggtggttccc ggatacaccg
     6781 ggctggatct ggagtgcgtt gttcctcggc gttatcttcc tgctgaacta catctcagtt
     6841 cgtggctttg gtgaagcgga atactggttc tcactgatca aagtcacgac agttattgtc
     6901 tttatcatcg ttggcgtgct gatgattatc ggtatcttca aaggcgcgca gcctgcgggc
     6961 tggagcaact ggacaatcgg cgaagcgccg tttgctggtg gttttgcggc gatgatcggc
     7021 gtagctatga ttgtcggctt ctctttccag ggaaccgagc tgatcggtat tgctgcaggc
     7081 gagtccgaag atccggcgaa aaacattcca cgcgcggtac gtcaggtgtt ctggcgaatc
     7141 ctgttgttct atgtgttcgc gatcctgatt atcagcctga ttattccgta caccgatccg
     7201 agcctgctgc gtaacgatgt taaagacatc agcgttagtc cgttcaccct ggtgttccag
     7261 cacgcgggtc tgctctctgc ggcggcggtg atgaacgcag ttattctgac ggcggtgctg
     7321 tcagccggta actccggtat gtatgcgtct actcgtatgc tgtacaccct ggcgtgtgac
     7381 ggtaaagcgc cgcgcatttt cgctaaactg tcgcgtggtg gcgtgccgcg taatgcgctg
     7441 tatgcgacga cggtgattgc cggtctgtgc ttcctgacct ccatgtttgg caaccagacg
     7501 gtatacctgt ggctgctgaa cacctccggg atgacgggtt ttatcgcctg gctggggatt
     7561 gccattagcc actatcgctt ccgtcgcggt tacgtattgc agggacacga cattaacgat
     7621 ctgccgtacc gttcaggttt cttcccactg gggccgatct tcgcattcat tctgtgtctg
     7681 attatcactt tgggccagaa ctacgaagcg ttcctgaaag ataccattga ctggggcggc
     7741 gtagcggcaa cgtatattgg tatcccgctg ttcctgatta tttggttcgg ctacaagctg
     7801 attaaaggaa ctcacttcgt acgctacagc gaaatgaagt tcccgcagaa cgataagaaa
     7861 taagtttcct cccttccttg ctaagccctc tcaaccgaga gggctttttc aattccattt
     7921 ccctgacaaa tcatgcggat ataaaattta acatttggat tgataattgt tatcgtttgc
     7981 attatcgtta cgccgcaatc aaaaaaggct gacaaatcag aggctgttcc ggctttctgg
     8041 gatgatcacc tgcatacaaa taagtccacc gcgatgctgc cgtacgcaag gggacgtgaa
     8101 gaaggtgtga gcgataaccc attttatttt cgtagttacc tcatggagat atggaatgtt
     8161 taggttgaac cctttcgtac gggtcaggct gtgtttgtcc gctatttctt gtgcatggcc
     8221 tgtgttagcg gtcgatgatg atggcgaaac gatggttgtc actgcatctt ccgttgaaca
     8281 aaacctcaaa gatgcacccg ccagtatcag cgtcattacc caggaagacc tgcagcgaaa
     8341 accggtacag aatctgaagg atgtcctcaa agaagtgcct ggcgtacaac tgacgaacga
     8401 aggggataac cgtaagggcg ttagtattcg tggtctggac agcagctaca ccctgattct
     8461 cgtcgacggt aaacgcgtta actcccgcaa tgccgtcttc cgccacaatg atttcgatct
     8521 gaactggatc ccggtcgatt ccatcgaacg tattgaagtg gttcgtggcc cgatgtcgtc
     8581 gctatacggc tccgatgcgc tcggcggtgt agtgaatatc atcaccaaaa aaatcggtca
     8641 gaaatggtcg ggtaccgtta ccgtcgatac caccattcag gaacatcgcg atcgcggtga
     8701 tacctataac ggtcagttct ttaccagtgg accattaatt gatggtgtgc tgggaatgaa
     8761 agcttacggc agcctggcaa aacgtgaaaa ggatgacccg caaaactcaa cgaccaccga
     8821 taccggagaa atgccgcgta ttgaaggatt ctccagccgc gacggcaatg tcgaatttgc
     8881 ctggacaccg aatcaaaatc acgattttac tgccggatac ggtttcgacc gtcaggaact
     8941 cgctggacaa aaaccgcctg gaacgccaga actactccgt cagccataat gggcgttggg
     9001 attacggcac cagcgaactg aaatactacg gtgagaaagt cgagaacaaa aaccctggca
     9061 acagcagccc gataacttcc gaaagcaata cggtcgacgg caaatacacg ttgccgctga
     9121 cggcgattaa tcagtttctc acggttggcg gtgaatggcg tcacgacaaa cttagcgatg
     9181 cggtgaacct gaccggggga accagctcca aaacgtctgc cagccagtac gcgctgtttg
     9241 tggaagatga atggcggatc ttcgagccgc tggcgctgac gaccggcgtg cgtatggacg
     9301 atcacgaaac ctacggtgaa cactggagtc cgcgtgccta cctggtttat aacgccaccg
     9361 acaccgtaac ggtgaaaggg ggctgggcga cggcatttaa agcaccttct ctgttgcaac
     9421 ttagccctga ctggacgagc aattcctgcc gtggcgcatg taagattgtg ggtagcccgg
     9481 atctgaaacc agaaaccagc gaaagttggg agctggggct ttactacatg ggtgaagaag
     9541 gctggctgga agggggtgaa tccagcgtta ccgttttccg taacgatgtg aaagatcgta
     9601 tcagcatcag ccgtacgtct gacgtcaacg ctgcaccggg ctaccaaaac tttgttggtt
     9661 ttgagacggg cgctaacgga cggcgcatac cggtatttag ctactacaac gttaacaaag
     9721 ctcgtattca gggcgtggaa accgaactga aaattccgtt caacgatgaa tggaaactgt
     9781 cgatcaacta cacctacaac gatggtcgtg atgtcagcaa cggcgaaaac aaaccgctat
     9841 ccgatctgcc gttccatact gctaacggta cgctggactg gaaaccgctg gcgctggaag
     9901 actggtcatt ctatgtttct ggtcactata ccgggcagaa acgcgccgac agcgcgacgg
     9961 ctaaaacacc gggcggttat accatctgga ataccggcgc ggcctggcag gtgactaaag
    10021 acgtcaaact gcgcgcaggc gtgctgaacc ttggcgacaa ggatctcagt cgtgacgact
    10081 acagctataa cgaagacgga cgtcgttact ttatggcagt ggattatcgc ttctgatgag
    10141 aagatgcccg gcgaaccggg cggactttca cttcagtaaa tactgcgcat ggaagcgcag
    10201 gtgatcctct ataaaagagg cgatgaagta gtaactgtga tcatatcccg gctgaatacg
    10261 cagcgtcatc ggccacgctt tctggcgcgc ggcttctgcc agtaccgcag gttgcaactg
    10321 gtcggcaaga aactgatcat tatcgccctg atcgataagc gtcgggatcg catcctgcgc
    10381 gttactggca tacatcagcg cgcaactgtc ccattccagc catgcatttt tgtcctcacc
    10441 taaatagctg ctaaacgctt tgattcccca cgggacgctg cacggattca caattggcgc
    10501 aaaggccgaa acgctggtgt atttacccgg atttttcagc gccataatca gcgcaccgtg
    10561 accacccatt gagtgaccgc taatggcgca gcggtcgctg acattaaatt gcgactgaac
    10621 cagcgccggt aattcatcgc gcagataatc atacatccgg taatgcgtcg cccacggcgg
    10681 ttgcgtggca ttaagataaa agcctgcgcc ctggcctaaa tcgtagccat cgtcgttggc
    10741 aaccttttcg ccgcgcgggc tggtgtctgg catcaccagt acaatcccca gttctgccgc
    10801 cacgcgctgg gcacccgcct tggtggtgaa gttctcgtca ttgcaggtta atccggaaag
    10861 ccagtacagc actggtggcg gggtgtgatc acgagggggt gggagaaaga tactgaacgt
    10921 catcgggcag tttaaggtac tggagtcgtg tcgccagcgt tgctgccagc cttcaaaaca
    10981 gcggtgctct tcgagcattt ccatgcgtgg ctccttgttg tgttgtttgc aatttcttca
    11041 tcataccgtt tattcaatgt gctgtgagta actttcactt ccgtatttgc ataacgatgt
    11101 tttaacatct gctgatgaaa ggcagcggca attacaataa ttatcgctgt gaatactgga
    11161 ttatgtgcgc cgcctcacgc acaataatca ggctgtaaat cagcttaata actttgcccc
    11221 cacgcagggc ggaggcgtca cacctgcagg agaaatcata aatgccatca ctcagtaaag
    11281 aagcggccct ggttcatgaa gcgttagttg cgcgaggact ggaaacaccg ctgcgcccgc
    11341 ccgtgcatga aatggataac gaaacgcgca aaagccttat tgctggtcat atgaccgaaa
    11401 tcatgcagct gctgaatctc gacctggctg atgacagttt gatggaaacg ccgcatcgca
    11461 tcgctaaaat gtatgtcgat gaaattttct ccggtctgga ttacgccaac ttcccgaaaa
    11521 tcaccctcat tgaaaacaaa atgaaggtcg atgaaatggt caccgtgcgc gatatcactc
    11581 tgaccagtac ctgtgaacac cattttgtta ccatcgatgg caaagcgacg gtggcttata
    11641 tcccgaaaga ttcggtgatc ggtctgtcaa aaattaaccg tatcgtgcag ttctttgccc
    11701 agcgtccgca ggtgcaggaa cgtctgacgc agcaaattct tattgcgcta caaacgctgc
    11761 tgggcaccaa taacgtggct gtctccattg atgcggtgca ttactgcgtg aaagcgcgtg
    11821 gcatccgcga tgcaaccagt gccacaacaa cgacctctct tggtggattg ttcaaatcca
    11881 gtcagaatac gcgccacgag tttctgcgcg ctgtgcgtca tcacaactga ttaaaaggca
    11941 ggaaccatgg agcgcaacgt aacgctcgat tttgttcgcg gcgtcgccat tctggggatc
    12001 ctgctattaa acatcagcgc ctttgggcta ccaaaggcgg cttatctcaa tcccgcctgg
    12061 tacggcgcta ttacgccgca ggatgcatgg acctgggcat ttctcgatct catcggccag
    12121 gtgaaattcc tcacgctttt tgcgctgctg tttggtgcgg gcctgcaaat gttgctgccc
    12181 cgtggcagac gctggatcca gtcgcggtta acgctgttag tcttgctggg ttttattcac
    12241 ggtttattgt tctgggacgg cgatattctg ctggcttacg ggctggtggg cttaatctgc
    12301 tggcggctgg tgcgcgatgc gccatcggta aaaagcctgt ttaatacagg cgtcatgctt
    12361 tatctggtgg ggcttggcgt tttgctgtta ttggggctga tttccgacag ccagaccagc
    12421 cgcgcctgga cgccggatgc atcggctatt ttgtatgaaa aatactggaa gcttcacggc
    12481 ggcgttgaag cgatcagtaa tcgtgccgat ggtgttggca acagtttact ggcactgggc
    12541 gcacagtatg gctggcaact ggcagggatg atgctcattg gtgcggcgct gatgcgcagc
    12601 ggctggctga aagggcagtt cagcttacgt cactatcgtc gtactggttt tgtgctggtg
    12661 gcgattgggg tgaccattaa ccttcctgcc atcgccctgc aatggcagct ggactgggcg
    12721 tatcgctggt gtgccttctt acttcaaatg ccgcgggaac tgagtgcgcc gtttcaggcg
    12781 attggctatg cgtcgctgtt ttatggcttc tggccgcaat tgagccgctt taagctggtg
    12841 cttgcgatcg cctgcgtcgg acggatggcg ctgaccaact atctattgca aacgctgatt
    12901 tgtaccatgc ttttttacca cctcggtttg tttatgcatt ttgaccgcct ggagctgctg
    12961 gcgtttgtta ttccggtatg gctggcgaat atcctcttct ctgttatctg gctgcgttac
    13021 ttccgccagg ggccggtgga atggctctgg cgtcagttaa ctttgcgtgc tgccggaacg
    13081 gcaatatcta aaacatcaag ataacgatct ggatcacatt cgttaacaaa acggctgtaa
    13141 ccgtttccat tgctgtgact cgattcacga agtcctgtat tcagtgctga caaaatagcc
    13201 gccagcaagc agtcatttac tgcaatctca taacaggtag tgaatatgat caccattcgt
    13261 gatgtagcgc gtcaggctgg cgtctctgtg gcaacggttt cccgggtgct caataacagc
    13321 acgctggtca gtgccgacac gcgtgaagca gtaatgaaag ccgtgagtga gctggattat
    13381 cggccaaacg ccaatgccca ggcgctggca actcaggtta gcgacaccat tggcgtggtg
    13441 gtgatggacg tttctgatgc gtttttcggc gcgctggtaa aagcggtgga tctagtcgct
    13501 cagcagcatc agaaatacgt gctaatcggc aatagctatc atgaagcgga aaaagagcgt
    13561 cacgccattg aggtgttaat tcgccagcgt tgtaatgcgt tgattgttca ctcaaaagca
    13621 ttgagtgacg atgaactggc gcaatttatg gataacattc ccggtatggt gttaatcaac
    13681 cgcgttgtgc cggggtacgc ccatcgttgc gtttgcctgg ataatctcag cggtgcccga
    13741 atggcgacgc gcatgttgct gaataacggt catcaacgta ttggttatct ttcttccagt
    13801 cacggcattg aagatgacgc catgcgtaaa gcaggctgga tgagtgcgtt gaaagagcag
    13861 gatattattc cgccggaaag ctggattggc actggtacgc cggacatgcc gggcggtgag
    13921 gcggcgatgg ttgaactgct ggggcgcaat ctacaactta ccgctatatt tgcttataac
    13981 gacaatatgg ccgctggcgc actgacagca ttaaaagata atggcattgc gattccgtta
    14041 catctctcaa tcatcggttt cgatgatatt cccatcgccc gttacaccga cccgcaatta
    14101 acgaccatgc gttatcccat tgcttcaatg gctaaattag ccaccgaact ggccttgcag
    14161 ggggcagcag gcaatattga tcctcgtgcc agccactgtt ttatgccgac gttagtgcgt
    14221 cgccattctg tcgcaacgcg ccagaatgcg gcggcgatca ctaactcaac aaatcaggcg
    14281 atgtaaccgc tttcaatctg tgagtgattt cacagtatct taacaatgtg atagctatga
    14341 ttgcaccgtt ttaacgttgt aacccgtatg taacagtgaa taatcacttt caccgaggta
    14401 atagcgtcat aacaacaatt aaagccgttt tctggagcgt taccgggcat ggaagaacga
    14461 attttaaaaa gtgagcttcg gcgttcagta acacttcatt aactctactg ccctgccgag
    14521 catttatctc aagcactacc ctgcataaga aaaaccggag ataccatgaa taagaaggtg
    14581 ttaaccctgt ctgctgtgat ggccagcatg ttattcggtg ccgctgcaca cgctgctgat
    14641 actcgcattg gtgtaacaat ctataagtac gacgataact ttatgtctgt agtgcgcaag
    14701 gctattgagc aagatgcgaa agccgcgcca gatgttcagc tgctgatgaa tgattctcag
    14761 aatgaccagt ccaagcagaa cgatcagatc gacgtattgc tggcgaaagg ggtgaaggca
    14821 ctggcaatca acctggttga cccggcagct gcgggtacgg tgattgagaa agcgcgtggg
    14881 caaaacgtgc cggtggtttt cttcaacaaa gaaccgtctc gtaaggcgct ggatagctac
    14941 gacaaagcct actacgttgg cactgactcc aaagagtccg gcattattca gggcgatttg
    15001 attgctaaac actgggcggc gaatcagggt tgggatctga acaaagacgg tcagattcag
    15061 tccgtactgc tgaaaggtga accgggccat ccggatgcag aagcacgtac cacttacgtg
    15121 attaaagaat tgaacgataa aggcatcaaa actgaacagt tacagttaga taccgcaatg
    15181 tgggacaccg ctcaggcgaa agataagatg gacgcctggc tgtctggccc gaacgccaac
    15241 aaaatcgaag tggttatcgc caacaacgat gcgatggcaa tgggcgcggt agaagcactg
    15301 aaagcacaca acaagtccag cattccggtg tttggcgtcg atgcgctgcc agaagcgctg
    15361 gcgctggtga aatccggtgc actggcgggc acagtactga acgatgctaa caaccaggcg
    15421 aaagcgacct ttgatctggc gaaaaacctg gccgatggca aaggtgcggc tgatggcacc
    15481 aactggaaaa tcgacaacaa agtggtccgc gtaccttatg ttggcgtaga taaagacaac
    15541 ctggctgaat tcagcaagaa ataagactga ttgggcgcag tcgtttactg cgcccttatt
    15601 gtatggccaa caaggtaaaa ttatatggtc agctcaacga ctccgtcttc cggggaatac
    15661 ttgttggaaa tgagcggtat caacaagtcc tttcctggtg ttaaggcact tgataacgtt
    15721 aatttaaaag tccggccaca ttctatccat gcattaatgg gggaaaacgg tgcaggaaaa
    15781 tcgacattat taaaatgcct gtttggtatt tatcaaaaag actccggcac cattttattc
    15841 cagggtaaag agatcgattt ccattccgcc aaagaggcac tggaaaatgg tatttcgatg
    15901 gtacaccagg agttaaacct ggtattacaa cgttcggtga tggataacat gtggctgagg
    15961 cgatatccca ccaaaggcat gtttgtcgat caggacaaaa tgtaccgcga aaccaaagcg
    16021 atttttgatg aactggatat tgatatcgat ccgcgtgcgc gcgtcggcac attatccgtt
    16081 tcgcaaatgc agatgatcga aatcgccaaa gcgttttcct ataacgcgaa aattgtgatt
    16141 atggatgaac cgacttcttc gttaaccgaa aaagaggtca atcatctgtt cactattatt
    16201 cgtaaattaa aagagcgcgg ctgcggtatt gtttatatct cgcataaaat ggaagaaatc
    16261 ttccagttat gtgatgaagt taccgtattg cgcgacggtc agtggatcgc caccgaaccg
    16321 ctggcaggac tgacgatgga caagatcatc gccatgatgg ttgggcgttc tcttaatcag
    16381 cgttttcctg acaaagaaaa caagccgggc gaagtcatcc tcgaggtgcg taacctaacg
    16441 tcactgcgcc agccgtcgat tcgcgatgtc tcgtttgatc tgcataaagg ggagatcctc
    16501 ggtattgccg ggctggtggg ggcgaaacgt accgatattg ttgagacgtt atttggtatt
    16561 cgcgagaaat cggctggcac cattacgttg cacggcaaac agatcaataa ccataatgcc
    16621 aacgaagcca taaaccacgg atttgcactg gtaactgagg agcgccgctc aacgggaatt
    16681 tatgcctatc tggatattgg ttttaactcg ttaatttcca atattcgcaa ctacaaaaat
    16741 aaagttggtt tactggataa atcgcggatg aaaagcgata cccagtgggt gattgattcg
    16801 atgcgggtaa aaacaccggg gcatcggacg caaattggtt cgctctcagg tggtaatcag
    16861 caaaaggtga ttattgggcg ctggctacta acgcaaccag aaatattaat gctcgatgaa
    16921 ccgacgcgcg gtattgatgt cggggcgaag tttgagatct atcagttaat tgccgaactg
    16981 gcgaagaaag gcaaagggat tattattatc tcctctgaaa tgcctgagtt gttagggata
    17041 acagaccgta ttctggtcat gagcaatggt ctcgtttccg gaattgtcga cacaaaaaca
    17101 acaacgcaaa acgaaattct gcgtcttgcg tctttgcacc tttaagatta ggggcttccc
    17161 atgagtgcgt taaataagaa aagttttctt acttacctga aagagggcgg tatttacgtc
    17221 gttcttttag ttttgctggc gattattatt ttccaggacc caacattttt aagtctgttg
    17281 aacttaagta atattctcac ccagtcatcg gtgcgtatta ttatcgcgct cggtgtggca
    17341 gggttaattg tcacccaggg gaccgacctt tctgcggggc gtcaagtagg gctggcagca
    17401 gtggtggcag cgacattatt gcagtccatg gataacgcca ataaagtgtt cccggaaatg
    17461 gcgacgatgc cgattgcgct ggttattctg attgtctgtg ccattggtgc ggtgatcggt
    17521 ttgatcaacg gtctgattat cgcttatctc aacgttacgc cgttcattac cacgctcggc
    17581 acgatgatca tcgtctatgg catcaactcg ctctattacg actttgtcgg ggcgtcgcca
    17641 atttctggtt ttgacagtgg cttctctacc tttgctcagg gctttgtcgc gctggggagt
    17701 ttccgtctct cttacatcac cttctacgcg ttgattgcgg tggcgttcgt ctgggtgttg
    17761 tggaacaaaa cccgcttcgg taagaacatt tttgccattg gcggtaaccc ggaagcggca
    17821 aaagtatctg gtgtcaacgt cggcctgaac ctgctgatga tctacgcgtt gtctggcgtg
    17881 ttctatgcct ttggcgggat gttagaagcc ggacgtatcg gctctgccac caacaacctc
    17941 ggctttatgt atgagctgga tgctatcgcg gcgtgcgtgg taggcggtgt atcgttcagc
    18001 ggcggtgtgg ggacggtgat tggcgtggtg accggggtaa ttatttttac cgtcatcaac
    18061 tatggcctga cgtatatcgg cgtaaaccca tgctggcagt acatcatcaa aggggcgatt
    18121 attatcttcg ccgtagcgct ggattcactg aaatacgcgc gtaagaaatg atgtgaatgt
    18181 ggtccgcatt cggtcataaa cgcacataac atcatgatct gtgtggtttt gtaggccgga
    18241 taaggcgttc acgccgcatc cggcaacagg agccaaatgc cggatgcaac gctggcgcgt
    18301 tttatccggc ctacaataag ctcaacatac tgatttgtat ggttttgtag gccggataag
    18361 gcgttcaagc cgcatccggc gacaggaacc aaatgccgga tgtgacgcgg gttctacaac
    18421 gttaccgggt gttctttctc gcctttctta aacttcactt ccccgagctc aatacaaccc
    18481 accgggcaga cgtgaccaca aagcagacaa cccacacatt tctcggtatt acaatgcggt
    18541 gtgcgggttt tctcgctcca ttccatcgcc tggtgactgc cgtcgtaaca ggaaatataa
    18601 cagcgtccac agccaacaca tttgtcaaga ttgatacggg gatagacaat ataactgcgg
    18661 tctaaatctt ccgccgggac aatattgtta ttcgccagac ctaccatctc ctgcaacgaa
    18721 tcaaatccct gatcggcgag ataatgcgat aacccgctcg ccatatcttc tacgatgcga
    18781 ttgccgtact gcataatgcc ggtggtgacc tgtaacgttg ctgcgccgag caataaaaac
    18841 tccgccgcat cctcccaggt ttcaatgccg ccgataccgc tgattgggaa atcgcgcagt
    18901 tctggatggg tgcgcatttg ctgaatgaag cgcagggcga tcggttttac cgctttaccg
    18961 gaatatccgg aaatactcga tttaccgtta acgatcggca taccgatttt ctgattaaga
    19021 tcgatattgg tgatggattt aacggtgtta attgccgcaa tgccatctgc gccgccgcgc
    19081 ttcgccgcca gcgccacttc gcacatatca ccgatattcg gcgtcatctt cgccagcatt
    19141 ggcagcgtgg aaccgcgttt caccgcccga caatattttt ctaccagcac cggactttgc
    19201 ccgacatcgc tacccatcgc atgagaagtc atttgcggac aggagaagtt acactcgatc
    19261 atatccgcgc cagctcctcc cattgctgct cattttcccc catgatcga
//
LOCUS       DADLFA010000069        19150 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000069 DADLFA010000000
VERSION     DADLFA010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19150
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.069"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..909)
                     /locus_tag="JQH65_002039"
     CDS             complement(<1..909)
                     /locus_tag="JQH65_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HAY6139456.1"
                     /translation="MTNFTTSTPHDALFKSFLTHPDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRPLPLVIPMLFYHGSRSPYPWSLCWLDEFAAPTTARKLYSAAFPLVDV
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVVLLVTECANDSQITALLNYIL
                     LTGDEARFKAFISELTRRMPHHRERIMTIAERIHNDGWLLGRERGRKEGKVEGERSLL
                     RLLLQNGADPEWIQRYTGLSAEQMQAL"
     gene            1376..2608
                     /gene="mdtM"
                     /locus_tag="JQH65_002040"
     CDS             1376..2608
                     /gene="mdtM"
                     /locus_tag="JQH65_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HAY6139457.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVFRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGSLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEER"
     gene            2649..3929
                     /locus_tag="JQH65_002041"
     CDS             2649..3929
                     /locus_tag="JQH65_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HAY6139458.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLSNF"
     gene            4045..5197
                     /locus_tag="JQH65_002042"
                     /pseudo
     CDS             4045..5197
                     /locus_tag="JQH65_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase subunit D"
     gene            5207..5975
                     /locus_tag="JQH65_002043"
                     /pseudo
     CDS             5207..5975
                     /locus_tag="JQH65_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5972..6229
                     /locus_tag="JQH65_002044"
     CDS             5972..6229
                     /locus_tag="JQH65_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HAY6139459.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGLTESIYCQQDGVWRCIAHYRVSPR"
     gene            6183..7154
                     /locus_tag="JQH65_002045"
     CDS             6183..7154
                     /locus_tag="JQH65_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiK family protein"
                     /protein_id="HAY6139460.1"
                     /translation="MVSGAALHITGFLRVKDSLRSLRILLDILLICLKGITVTKSISL
                     SKRISVIVILFAIVAVCTFFVQSCARKSNHAASFQNYHATIDGKEIAGITNNISSLTW
                     SAQSNTLFSTINKPAAIVEMTTNGDFIRTIPLDFVKDLETIEYIGDNQFVISDERDYA
                     IYVISLTPNSEVKILKKIKIPLQDSPTNCGFEGLAYSRQDHTFWFFKEKNPIEVYKVN
                     GLLSSNELHISKDEALQRQFTLDDVSGAEFNQQKNTLLVLSHESRALQEVTLVGEVIG
                     EISLTKGSRGLSHNIKQAEGIAMDASGNIYIVSEPNRFYRFTPKSSH"
     gene            7222..8394
                     /locus_tag="JQH65_002046"
     CDS             7222..8394
                     /locus_tag="JQH65_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY6139461.1"
                     /translation="MPSSSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVIMAE
                     GAFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLVSALARGLLILAMAWL
                     PPFILVLLIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIALGNEYVLAGL
                     HFAFSSQTLWQGAGALSGMMLIALTLLMPSKKHAIAPMPLAKTEQQIMSWWLLAILYG
                     LAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPCLT
                     ANLLVQAISVLLTLASDSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLLGF
                     VTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS"
     gene            complement(8407..9064)
                     /gene="kptA"
                     /locus_tag="JQH65_002047"
                     /pseudo
     CDS             complement(8407..9064)
                     /gene="kptA"
                     /locus_tag="JQH65_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709901.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
     gene            9212..9894
                     /locus_tag="JQH65_002048"
                     /pseudo
     CDS             9212..9894
                     /locus_tag="JQH65_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9891..10352
                     /locus_tag="JQH65_002049"
     CDS             9891..10352
                     /locus_tag="JQH65_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6139462.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            10365..11537
                     /gene="iadA"
                     /locus_tag="JQH65_002050"
     CDS             10365..11537
                     /gene="iadA"
                     /locus_tag="JQH65_002050"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAY6139463.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGSKPGVTVFHMGDSKKALQPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            11602..12513
                     /gene="hypT"
                     /locus_tag="JQH65_002051"
                     /gene_synonym="qseD"
     CDS             11602..12513
                     /gene="hypT"
                     /locus_tag="JQH65_002051"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HAY6139464.1"
                     /translation="MDDCCAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEYGEAIFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(12506..12898)
                     /gene="iraD"
                     /locus_tag="JQH65_002052"
     CDS             complement(12506..12898)
                     /gene="iraD"
                     /locus_tag="JQH65_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HAY6139465.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(12895..12978)
                     /locus_tag="JQH65_002053"
     CDS             complement(12895..12978)
                     /locus_tag="JQH65_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide"
                     /protein_id="HAY6139466.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            13570..14400
                     /locus_tag="JQH65_002054"
     CDS             13570..14400
                     /locus_tag="JQH65_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HAY6139467.1"
                     /translation="MSMPLSNALQSQIITDNHFLHHPKVDNKLTREYEYTRMDTENIY
                     LLPLTRGNNHNYDGKSVVEIRKLNIGKQSWPFNYATDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGYNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIGMGN
                     DPIIGIEAYPGTAEIHAKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(14541..15314)
                     /gene="uxuR"
                     /locus_tag="JQH65_002055"
     CDS             complement(14541..15314)
                     /gene="uxuR"
                     /locus_tag="JQH65_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HAY6139468.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(15529..16989)
                     /locus_tag="JQH65_002056"
                     /pseudo
     CDS             complement(15529..16989)
                     /locus_tag="JQH65_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
     gene            complement(17070..18254)
                     /gene="uxuA"
                     /locus_tag="JQH65_002057"
     CDS             complement(17070..18254)
                     /gene="uxuA"
                     /locus_tag="JQH65_002057"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAY6139469.1"
                     /translation="MEQTWRWYGPNDLVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            18594..18798
                     /locus_tag="JQH65_002058"
                     /pseudo
     CDS             18594..18798
                     /locus_tag="JQH65_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19041..>19150)
                     /locus_tag="JQH65_002059"
     CDS             complement(19041..>19150)
                     /locus_tag="JQH65_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY6139470.1"
                     /translation="TSAVSLGLTANYARTGGQVTAGNVQSIIGVTFVYQ"
BASE COUNT         4475 a         4870 c         4805 g         5000 t
ORIGIN      
        1 taatgcctgc atttgctctg ccgaaagtcc ggtatatcgt tgtatccatt caggatcggc
       61 cccattctgc aacaacaatc ggaggaggct ccgttcccct tctactttcc cttctttcct
      121 ccccctctcc cttcccaaca gccatccatc attatgaatt cgctctgcaa ttgtcattat
      181 tcgctccctg tggtgtggca ttcgcctggt aagttcgctg ataaacgcct taaaacgcgc
      241 ttcatcgcca gtcagtaaaa tgtaatttaa cagcgcagtt atctggctgt cattagcaca
      301 ctctgtaacc agtaatacta ccagttggtc aataagcccc atcaaatcgc gctggcgaat
      361 atgcttttgg atcaactcca acagggcgac tctgcgatgc tgcacaatct cgtcgtctgg
      421 cacgacagtg acatccacca gcgggaacgc tgcgctataa agtttccgtg cggtagtcgg
      481 ggcggcaaat tcgtccagcc agcacaggga ccaggggtaa ggactacggc taccgtgata
      541 aaacagcatc gggataacca gcggtagcgg ccggcgttta tcatgctcga tatggcgctg
      601 catcaccgcc atggaatatc gcatcaggcg aaatgccata tgaatatcct cgcggctctg
      661 atgttcaatc actacgtaaa tataaccatc accttcacgg gtctttaccg accacagaat
      721 atcggagtgt agcgcccgca atttttcatc gacaaagctg gcggattcca gttttaagct
      781 gtcgagatcg cacagttcac gtaaatcttt gggtaagtgg atctccataa aatcccgcgc
      841 ggtgtcaggg tgagtgagaa aggatttaaa taatgcgtca tgtggcgtgc tggtcgtgaa
      901 gtttgtcatg gcgatccgtc acctgtagat aagatggccc gactttagcg actaacaaaa
      961 ccattctcac ccggcaattt ctcgttcccc gaggtgcttt ccaggcgtct gcaaacgata
     1021 catttttccg gaaacaggct cgcgttttac atttcctgta cccggcagat attccccttt
     1081 ccaccgcgct cttttgatta accgcaaatt ttaattacac ttaaggtgta tattttctat
     1141 gcaacccatc aattaaagag gtgtaatgtg ctgatgacta tttgtaatcg ttatacattc
     1201 tgacctggag tcagaaggta tttctctgtc tgtgtgttca caggcagtgt ggttgattac
     1261 atgaaattca gtacatttgc agtctcgttg cccttctcgc ctgcctttcg tcattaccga
     1321 cggtattgaa tttcgttttc cccgttgggg ttctccgaac aaggagttgt ttgttatgcc
     1381 acgttttttt gcccgccatg ccgccacgct gtttttcccg atggcgttga ttttgtatga
     1441 ctttgccgcg tacctgtcga cggatctgat ccagccgggg atcattaatg tggtccgtga
     1501 ttttaatgcc gatgtcagtc tggccccggc agccgtcagt ctctatctcg cgggcggtat
     1561 ggcgttacag tggctgctgg ggccgctttc cgacaggatt ggccgcaggc cggtgctgat
     1621 taccggggcg ctaatattta cccttgcctg cgctgcgaca atgttcacaa cgtctatgac
     1681 acagtttctt atcgcgcgtg caattcaggg caccagtatc tgttttattg ccaccgttgg
     1741 ttatgtcacg gtgcaggagg cgttcggcca gacaaaaggg atcaagttga tggcgattat
     1801 cacctccatc gtactgattg cgccgattat tggcccgctt tccggcgcag ctctgatgca
     1861 ctttgtgcac tggaaagtcc tttttgccat cattgcagtt atgggtttta tctcatttgt
     1921 tggcttactg ctggcgatgc cagagacggt gaagcgcggc gcggttccat ttagcgccaa
     1981 aagcgtcttt cgcgattttc gtaatgtctt ttgcaatcgg ctgttcctct ttggcgcagc
     2041 aaccatctct ttaagctata tcccaatgat gagttgggtg gctgtctcgc cggtgatcct
     2101 gatcgacgcg ggcagcttaa caacttcgca gttcgcctgg acacaggttc cggtgttcgg
     2161 cgcggtgatt gtcgcgaatg ccatcgtggc gcgtttcgtt aaagatccga ccgaaccgcg
     2221 ctttatctgg cgtgctgtac ccattcaact ggtcggcctc gcgctgttga ttatcggcaa
     2281 tctgctgtca ccgcacgtct ggctgtggtc ggtgctgggc accagtttgt atgctttcgg
     2341 gattggtttg atcttcccga ccttattccg ctttacgctt ttttccaata acttaccgaa
     2401 agggaccgtc tccgcatcgc tgaatatggt gatcctgatg gtgatgtctg tctcggtcga
     2461 aatcggccgc tggctgtggt ttaacggcgg tcgcttgccg tttcatctgt tagccgtcgt
     2521 ggcgggcgtt atcgtcgttt tcaccctggc aggattgctt aatcgcgtgc gccaacatca
     2581 ggctgccgag ctggcggagg agcggtgatt tttgcgcgat cctgccgtca ggctctattc
     2641 ttaacgttat gaataaactc attgaactca gacgcgccaa aatgttggcg ctctctttac
     2701 tgcttatcgc cgctgctacc tttgtcgtta cgctgttttt gccgcccaat ttttgggtga
     2761 gcggcgtgaa ggcgattgct gaagcggcga tggtcggcgc gctggcggac tggtttgcgg
     2821 tggtggcgct gtttcgccgc gtgccgattc cgatcatttc tcgtcatacg gcgattatcc
     2881 cgcgtaataa agaccggatt ggcgaaaatc tcggtcagtt cgtgcaggaa aaatttctcg
     2941 atacccagtc gctggtggca ttgattcgac gtcacgaacc ggcgttgttg attggcaact
     3001 ggtttagtca gccggagaac gcccgccgcg ttggtcagca tctgttgcag atcatgagcg
     3061 gttttcttga actgaccgat gatgcgcgta ttcagcgcct gcttaagcgc gcggtccatc
     3121 gggcgattga taaggtcgat ctttccggaa ccagtgcgtt gatgctggag agtatgacca
     3181 aaaacgatcg tcatcaggtg ctgctcgata cgctgatcgc acagttgatc gcccttctcc
     3241 agcgcgataa atcgcgcaag tttattgccc agcaaattgt tcgctggctg gagagcgagc
     3301 atccgctgaa agccaaaatt ttgcctactg aatggctggg cgaacatagc gcggagttgg
     3361 tttctgacgc ggtgaattct ttgcttgatg atatcagccg cgatcgtgcg catcagatcc
     3421 gtcatgcgtt tgatcgcgcc acttttgccc tgatcgacaa gctgaaaaac gatccagaaa
     3481 tggcagcgcg agccgatgcc gtaaaaagtt atctgaaaga agatgaagct tttaaccgct
     3541 atctcagtga attgtggggg gatttacggg aatggctgaa agcggatatc aacagtgaag
     3601 attctcgtgt gaaagaacgt atcgcgcggg cgggtcaatg gtttggcgaa acgttaattg
     3661 ccgatgatgc cttgcgggca tcgttaaatg gtcacctgga acaagccgcg caccgcgtcg
     3721 cgcctgagtt ttccgcattc ctgacgcgcc acatcagcga tacagtaaaa agctgggatg
     3781 ctcgggatat gtcgcggcaa atcgagttaa atatcggcaa agatctgcag tttatccgtg
     3841 tcaacggtac gctggttggc ggttgtattg ggctaatttt atatttgttg tcgcagctcc
     3901 cggccttgtt ccccctcagc aatttttaga aatccatgaa aaactaagtg gctaaccgcg
     3961 agagagatca aataaaccac actaaaagaa tggtaataat gtggcctctt ttcattatct
     4021 cccgtggtac ggggaaggaa aatcatgtca cttatcaccg atctacccgc cattttcgat
     4081 cagttctctg aagctcgcca gaaaggcttt ctcaccgtca tggatctcaa ggagcgcggc
     4141 attccgctgg ttggcactta ctgcaccttt atgccgcaag agatcccgat ggcagccggt
     4201 gcggttgtgg tttcgctctg ttctacctct gatgaaacca ttgaagaagc ggagaaagat
     4261 ctgccgcgca acctctgccc gctgattaaa agcagctacg gcttcggcaa aaccgataaa
     4321 tgcccctact tctacttttc ggatctggtg gtcggtgaaa ccacctgcga cggcaaaaag
     4381 aaaatgtatg aatacatggc ggagtttaag cccgttcatg tgatgcagtt gccgaacagc
     4441 gttaaggacg atgcctcgcg tgcgttatgg aaagccgaga tgctgcgctt gcaaaaaacg
     4501 gtagaagaac gttttgggca cgagattagc gaagatgctc tgcgcgatgc cattgcgctg
     4561 aaaaaccgcg aacgtcgcgc actggccaat ttttatcatc ttgggcagtt aaatcctccg
     4621 gcgcttagcg gcagcgacat tctgaaagtg gtctacggcg caaccttccg gttcgataaa
     4681 gaggcgttga tcaatgaact ggatgcaatg accgcccgcg ttcgtcagca gtgggaagaa
     4741 ggccagcgac tggacccgcg tccactcatt ttaatcaccg gctgcccgat tggcggcgca
     4801 gcagaaaaag tggtgcgggc gattgaagag aatggcggct gggttgtcgg ttatgaaaac
     4861 tgcactgggg cgaaagcgac cgagctaatg cgtggcggaa acgggcgatg tctacgacgc
     4921 gctggcggat aaatatctgg cgattggctg ctcctgtgtt tcgccgaacg atcagcgcct
     4981 gcaaatgctc agccagatgg tggaagaata tcaggtcgat ggcgtagttg atgtgatttt
     5041 gcaggcgtgc catacctacg cggtggaatc gctggcgatt aaacgtcatg tgcgtcagca
     5101 gcacaacatt ccttatatcg ctattgaaac agactactcc acctcagatg tcgggcagct
     5161 cagtacccgt gtcgcggcct ttattgagat gctgtaagga gtggcagtga catattcgat
     5221 tggcattgat tctggttcta ccgccaccaa agggatctta ctggcagacg gcgtgattac
     5281 gcgccgtttc ctcgttccaa caccctttcg tccggcaaca gcaattactg aagcctggga
     5341 aactctgcgc gaagggttag agacaacgcc gtttctgacg ctcaccggct atggacggca
     5401 actggtggat tttgccgata aacaagtgac ggaaatctcc tgtcacgggc tgggcgcacg
     5461 gtttcttgcg ccagcaacgc tgcgcggtaa tcgacatcgg tggtcaggac agcaaagtga
     5521 ttcagcttga tgatgacggt aacctgtgcg atttcctgat gaatgacaaa tgcgcggcgg
     5581 gcaccgggcg tttcctggaa gtgatctcgc gcacgcttgg caccagcgtc gagcaactcg
     5641 acagcattac cgaaaatgtc acgccgcatg ccatcacgag tatgtgcaca gtgtttgccg
     5701 aatcagaagt gatcagcctg cgctcagcgg gcgtcgcgcc agaagcgatt ctcgccggag
     5761 tgattaacgc gatggcgcgg aggagtgcca atttcattgc tcgtctctcc tgtgaagcgc
     5821 cgattctgtt tactggtggc gttagtcatt gccagacgtt tgcccggatg ctggaatctc
     5881 acctgggaat gccggtaaat acccatcctg atgcgcaatt tgctggcgca attggcgcgg
     5941 cggtaattgg tcaacgagtg aggacacgcc gatgaaagag tttttatttt tatttcactc
     6001 cacagtcggc gtcatacaaa ctcgcaaagc gttgcaggca gcgggcatga cttttcgcgt
     6061 cagcgatatt ccccgtgatt tacgcggcgg ctgcgggtta tgcatttggc tgacctgtcc
     6121 ccccggcgaa gaaatacaat gggtgatccc tgggctgact gagtcaattt attgccagca
     6181 ggatggtgtc tggcgctgca ttgcacatta ccgggtttct ccgcgttaag gattcattaa
     6241 ggtcgctacg tatacttctc gatatcttat tgatttgcct taaagggatt accgtgacaa
     6301 aaagtatttc gttaagtaaa cgcatctccg ttattgtgat tctttttgcc attgtggcag
     6361 tgtgtacttt cttcgtgcaa tcttgcgccc gtaaaagcaa tcatgcagca tcatttcaga
     6421 attaccacgc caccattgac ggcaaagaaa ttgcaggcat aacgaataat atctcttcgc
     6481 tgacctggtc ggcacaaagc aatactcttt ttagcactat caacaaacct gccgccattg
     6541 ttgaaatgac caccaatggt gattttatcc gcacaattcc tctggatttt gttaaggatc
     6601 tcgagacgat cgaatacatt ggcgataatc agtttgtcat tagcgatgag cgcgattacg
     6661 ccatttatgt gatttcactg accccaaact cggaagtaaa aatcctcaaa aaaataaaaa
     6721 tcccgctgca agactcccca accaattgcg gttttgaagg gttagcttat tctcgccagg
     6781 atcacacttt ctggtttttt aaagagaaaa atccgattga ggtctacaaa gttaacgggc
     6841 tgttaagtag caatgaatta cacatcagca aagacgaggc actacaacgg caatttactt
     6901 tagatgatgt gtccggcgca gaatttaacc agcaaaaaaa tacgcttctg gtgctgtctc
     6961 atgaatctcg cgcactccag gaagtaaccc tggttggcga ggttatcggt gaaatatcac
     7021 taaccaaagg tagtcgaggt ttgtcgcata acatcaaaca ggccgaaggg attgcgatgg
     7081 atgcctctgg taatatttat atcgttagcg aaccaaatcg tttttatcgc tttaccccta
     7141 aatcatcgca ttaacaggct ggcatatttc acctttttgt tattaacttg ttaacaacaa
     7201 aaatgacata caaggttaaa catgccttcg tcctcgcatc ccgtagaacg cttttctttc
     7261 agcaccgcgc ttttcgggat gctggttctg accctaggta tgggattagg tcgcttcctt
     7321 tatacgccta tgttgcccgt cattatggcg gaaggggcgt tttcatttag ccagctctcg
     7381 tggattgcca gcggcaacta tgcggggtat ctggctggca gtctgctatt ttcgtttggc
     7441 gcatttcacc agccgtcgcg cctacgccca tttctgttgg tttctgccct ggcgagaggg
     7501 ttgttgatcc tcgcaatggc atggttgccg ccatttattc tggtgttatt gattcgcttc
     7561 ctggcgggtg tcgccagcgc cggtatgctg atttttggtt cgacgctgat tatgcagcac
     7621 acgcgccatc cttttgtgct ggcagctttg ttttctggcg ttggcattgc actgggcaac
     7681 gaatatgttc tggcaggcct gcattttgcc ttctcctcgc aaacgttatg gcaaggcgcc
     7741 ggggcgcttt ctggcatgat gctgattgca cttacgcttt taatgccctc gaaaaaacac
     7801 gccatcgcac caatgccatt ggcaaaaacg gagcaacaga taatgagctg gtggttactg
     7861 gctattctgt acggccttgc gggatttggt tatatcatcg tcgcgactta cctgccgctc
     7921 atggcgaaag atgcaggctc accattgtta accgcccatc tctggacgtt agtcggctta
     7981 tcaattgtgc caggctgctt tggctggcta tgggcagcaa aacgttgggg ggctttgcct
     8041 tgcctgacgg cgaatttgct ggtgcaggct atcagtgtgc tgcttactct cgccagcgac
     8101 tctcctctct tgcttatcat tagtagtatc ggttttggcg gtacctttat gggcacgaca
     8161 tctctggtga tgactatcgc ccgccaactg agcgttccgg gaaatctcaa tcttttaggc
     8221 tttgtgacgc tcatttacgg cattggacaa atcctcggcc cggcgctgac cagtatgctc
     8281 ggcaatggaa catcggcgct tgccggcgcc actctctgcg gcgcggcggc gttgtttatt
     8341 gctgcgctta tcagcacagt acaattattt aagctgcaag tggtcacttc atgaggagtg
     8401 tttcccttac cactcaagaa attcgaccgc aaccgttgat gtcagccaga ctccgttttc
     8461 cgcttgccag aaaggaatgc ctcgtttcgc catttcctgt gctttgacgg ttaagacaac
     8521 cggcgatccg tgtcttgctc ccactttgcg tgccgttgct tcatcagcag agagatgcac
     8581 gtaatgacga tcccctgcaa tcagcccctg ttttttttat ctcgtcaagg aaacggcttg
     8641 ccgtaccgtg gtagagaaac tgtggcggtg ttttttcggc aaaggaaata gcgacctgcg
     8701 aagtcgaatg cccctgaacc gcgcggatgc acctaccatc actggaataa ctaaaacgct
     8761 ttttatcgct ggttgcgacc acggtatcca gcaatgcacg ggttaatcgc ttgcccgctt
     8821 tttgggcaca aagtatgagc ttatcgatat ccgcccatcc ttcacggtcc agtacgatac
     8881 caatcgcctc cggtttgtgt cggaggacaa aacttaaaaa cttgctcgta tccgccagct
     8941 ctttttcgtt gtgctttgcc atctcgtttt cgtccctgga ttttctgttt ttatttacca
     9001 atgtcattgt cactgggttt atgaattttc tgcaactcaa tcctgcgcag aaccttttcc
     9061 acacagcgta atgtttcccc tatgcaaaaa acgcatagcc agaaacatac ttgcattttc
     9121 cccataccca catggctgat aaggtttttc agttccagga attttcctgg ctcaagcaac
     9181 acacaaacac aaaaaaacaa tggggattgt tatgacacaa caaggggatg ctgtagccgg
     9241 ggagctggcg acagaaaaag tgtgcattaa agggtatctg gctttttttc tcaccataat
     9301 cttttttccg gtgttttttc cggaacagac agctggtggc gcgtttttga tttttccgtt
     9361 ctgaacggat cgtttgggca actgcctggt gcgaacggag ccaccacctc atttcgcggc
     9421 gcgggtggtg cgggggctaa agatggcttt ctctttgcgc tggagctggc accgtcagtc
     9481 attctctctc tggggattat ttcaattacc gatggtcttg gtggattacg cgccgcgcag
     9541 cagttaatga cacctgtgct aaaaccgttg ttgggcattc cagggatctg ctcgctggcg
     9601 ctgatcgcta acttgcaaaa cactgatgcc gccgccggta tgaccaaaga gctggcgcag
     9661 gaaggtgaaa ttaccgaacg cgataaagtg attttcgccg catatcaaac cagcggtagc
     9721 gccatcatca ccaactactt ctcttccggc gtcgcggtgt tcgcctttct gggcacgtcc
     9781 gtaatcgtac ctttagccgt catcctggtg tttaaatttg tcggagccaa cattttgcgc
     9841 gtctggctta acttcgaaga acgtcgcaac ccaacgcaag gagcgcaagc atgacaactc
     9901 aggtccgcaa aaacgtcatg gatatgttta tcgatggcgc gcgccgtggc tttactatcg
     9961 ccacgaccaa cctgctgcca aacgtggtga tggcgtttgt catcattcag gcgctgaaaa
    10021 ttaccggcct gctcgactgg gtggggcata tctgtgaacc cgtaatggcg ctgtggggat
    10081 tacccggtga agcggcaacc gtccttttag ccgcattaat gagtatgggg ggcgcagtcg
    10141 gtgttgccgc cagtctggca accgctggcg cattaaccgg tcacgatgtc accgttttac
    10201 tgcccgcgat gtatttaatg ggcaacccgg tgcaaaatgt cggtcgctgc ctggggacag
    10261 ccgaagtgaa tgccaaatat tacccgcata tcattacggt ctgcgtgatt aacgcattgc
    10321 tgtcgatctg ggttatgcag cttattgttt aacaaggagt taccatgatt gattataccg
    10381 cagccggttt taccctgctg cagggagcgc atttgtatgc gccggaagat cggggaattt
    10441 gcgatgtcct cgtcgctaac ggcaaaatta tcgccgttgc cagcaatatc ccttctgaca
    10501 ttgtaccgaa ctgcacggtt gtcgatctca gtgggcagat cctctgccca ggttttattg
    10561 atcaacacgt ccatttgatt ggcggtggcg gcgaagcagg tcccacgacg cgcacgccgg
    10621 aagtggcgct aagtcgcctg acggaagcgg gcgtcacgtc agtggttggt ctgctgggca
    10681 ccgactctat ctctcgccac ccggaatccc tgctcgccaa gacccgtgcg ctcaatgaag
    10741 aaggcatcag cgcctggatg ctgaccggcg cttatcatgt cccttcccgc actattacgg
    10801 gttccgtgga aaaagacgtg gcgattatcg atcgtgtgat tggcgtgaaa tgcgccatct
    10861 ctgatcaccg ttctgccgca ccggacgttt atcacctggc taatatggcg gcagaatccc
    10921 gcgttggcgg tttgctcggc agtaaacctg gcgtcaccgt gttccacatg ggcgacagta
    10981 aaaaggcgtt acagcctatt tatgacctgc tggaaaactg cgatgtgccg atcagcaagc
    11041 tgctgccgac ccacgttaac cgcaacgtac cgttgtttga gcaggcgctg gagttcgcgc
    11101 gcaaaggcgg caccatcgat atcaccagca gcattgacga accggtcgcc cctgccgaag
    11161 gtattgcccg cgccgttcag gcgggtattc cgctggcacg cgtcaccctc agctccgacg
    11221 gcaacggtag ccagccgttc ttcgatgacg aagggaattt aacccatatc ggtgttgcag
    11281 gttttgaaac gttgctggaa accgtgcagg tgctggtcaa agactatgat ttcagtatca
    11341 gcgatgccct gcgcccgctc accagtagcg tagccggttt ccttaacctg agcgggaaag
    11401 gcgaaattct gcctggcaat gacgcagact tactagtcat gacgccagaa ttgcgcattg
    11461 agcaggtata tgctcgcggc aaactgatgg tcaaagacgg caaagcctgc gtgaaaggaa
    11521 cgtttgaaac ggcttaaacg gttgtcagcc gccctaatgg acgtagaatg cccgcaaggg
    11581 cggctgacag agtaaaacgt aatggatgac tgttgtgcgg ttttgcataa tattgaaacc
    11641 aaatggcttt atgattttct gaccctggaa aaatgccgca atttttccca ggcggcagtc
    11701 agtcgtaacg tctcgcaacc ggcattcagc cgccgcatcc gcgcgctgga acaggcgatt
    11761 ggcgttgaat tgtttaaccg ccaggtgacg ccgctgcaac tctcagaaca agggaaaatc
    11821 ttccattcgc agatccgcca tctgttgcaa cagttagaaa gtaaccttgc agagctgcgt
    11881 ggcggcagcg attacgcgca acgtaaaatc aagatagccg ctgcacactc tctttccctc
    11941 ggtctgttac cgtccattat cagccagatg ccgccgctct ttacctgggc gattgaagct
    12001 attgatgtcg atgaagcagt cgataaactg cgtgaagggc aaagtgactg tattttttcc
    12061 tttcacgacg aagatttgct ggaagcaccg tttgaccaca ttcgcttatt tgaatctcaa
    12121 ttgttccccg tttgcgccag tgacgaatac ggagaagcaa tttttaacct cgcgcagcca
    12181 cactttccgt tactgaatta cagccgcaac tcctacatgg ggcgattgat taatcgcacc
    12241 ctgacgcgcc acagcgagtt aagtttcagc accttttttg tctcttcgat gagcgagcta
    12301 ttaaagcagg ttgccctcga cggctgtggg attgcctggc tgccggagta tgccatccaa
    12361 caagaaattc gcagcgggca actcgttgtg ctgaatcggg acgaactggt gatcccgatt
    12421 caggcctacg cataccgtat gaatacccgc atgaatccag ttgccgaacg cttctggcgt
    12481 gaactgcgcg agctggagat tgtgcttagc tgacattctc aagcgtcgca ctgcgccaac
    12541 cgccattatc ccagaccaga tcaaacgtta gcggttcatc gtcgtggtac agcattatct
    12601 caaggctgat cgccagagtg tggcagtacg cttctttcaa attaacctgc aacgccgcaa
    12661 tccgtggctc atgaaataac agtgcagaac gcagctgacg gcaataccag ctctggagat
    12721 gtttgtcgct cgggttatgc acttgccact cgggcatgcc tgaaacaagt aatgacggta
    12781 atgccgaatg aggagaatta aaaagagtga ggagatcgct gcataccgat ttacgcagca
    12841 atgacgtttt attgcctgaa atttcaggta aaacagcctg aagtgattgg cgcatcattt
    12901 tgcgcactcc tgacgtttag caacatatgc ggcttgccct gaacaccggg caccactgta
    12961 ttgatgctca ttttccatag ccattccctt ttgttcatta catccatgtc agtctgagac
    13021 aggcctgaat cctgtcagca aagtataaaa ccttacttgc ctatgaatat ctactgactt
    13081 ttcaggaaaa aagtcatgat ttgataaaaa tcatcaaact caaaaaaaaa ccgctagtat
    13141 attctgaata ttccgaatag ttacgtagat gaaaatactt gtcaccgccg ctacttaatt
    13201 ccggtgggaa attatttatt tccgcataat cagccatgta ttatttgaaa caattagttt
    13261 gtaaataata taaattgaca acatatcaaa tacatcaaaa ataccattca taggcattat
    13321 taatatagac atcaatatag caacatcaaa ttccaattag taataagata tgctttttaa
    13381 ttgcatgaaa ataatttcaa atgagcgttt tttgatactt tagagtgttt cctacccttt
    13441 aatatcaccg tgtagagttg atatcactac aacagaaaca tgcatgtcat ttttttactt
    13501 cttttttact acagaaaaat ttatctattt ttctaaacat cggtgaaata ctcataaaag
    13561 gtaactatta tgtctatgcc attaagcaat gcccttcaga gtcaaattat tacagataat
    13621 cacttccttc atcatccaaa agtcgacaat aagttaacac gtgaatatga atatacacgc
    13681 atggatacgg aaaatatcta tcttctgcct cttactcgtg gaaataacca taattacgat
    13741 ggaaaaagcg ttgtcgaaat tagaaaactt aatattggca aacagtcctg gccatttaat
    13801 tatgcgacag atacatgcag agaattcaat ggtattacaa caacaggacg tatgctctat
    13861 cgaaacttga aaatcacctc agctttagac gaaatatatg gtggcatatg taaaaaggcg
    13921 catgctgcta cagagttagc agaaggttta cggctaaatt tatttatgaa gagccctttc
    13981 gatcctgttg aggactatac agttcatgaa ataacattag gccctggtta caatgttcct
    14041 ggttatgccg gaaccacgat aggatatatt tcaacccttc cagcttctca ggcaaaaaga
    14101 tggaccaatg agcagccaag aattgatatc tatattgatc agataataac tgtctcaggg
    14161 gtggcaaatt cttccggatt cgcccttgcc gcattactta atgctaatat tggaatggga
    14221 aatgacccaa taattggtat agaagcctat cctggtaccg cggagatcca tgcaaaaatg
    14281 ggatataacg ttatccccgg tgatgaagat gcgccactca aacgcatgac tctgcaaccg
    14341 tcatctcttc cagaactgtt tgaattaaaa aatggtgaat ggaattatat tggcaaataa
    14401 ccactgagaa gaatgagtac taacattcag gttatataga tatttaaaca ataccctgca
    14461 atttacaaag taatactcat tgcgacttct taatttaaga aagcgcaagg aacgtttacc
    14521 cttgcgctta tcataatcag tcaggcgtcg actttatcca gcggccatga atcaaacaga
    14581 tagccgtcaa aataaatatc gtcaacgttc gagaattcca gcagacgttg cttaacgttt
    14641 tccagatgct gccacattgc cagcttcgcc gctcgggcat cttttttgat taacgcggca
    14701 aggatctgtt tgtgatcgcc caaccactct ttgcgataca ggctgtcatc cagatggctg
    14761 tgcaactgga tccacattgg attgttttcc cgccactgcc aggactgacg gaataactcc
    14821 accagcatgc tgttatgcgt tgcttcagca atggcgagat ggaactgcat gtcaccgctt
    14881 tcgctgctac ccggcgcgct ggaagccagt tcacgctctt ccagttgcaa tgcctgacgc
    14941 attttgacga tatcttcgcg ggtagcctgc agggcggcaa attccgcgat gttgctctca
    15001 agaagctgtc gcgcctgcaa caattcaaag gggccggcat cattgcagac gttggcatcc
    15061 ggactgtctg tgttgtggct gcctgagcta tcaagaacat agataccggc accccggcgc
    15121 acttccacca gccctttgat ctccagcatg atcagcgctt cacgtaccac cgtccgcgtg
    15181 acatcaagca tttctgcaat ttcacgctcc ggcggcagcc gttcgccagg attgtacggc
    15241 gtctttatga tcagatcgcg gatcatcgcc ccgacttcct ggtaaggtct ttgcgcagag
    15301 gtggcagatt tcatggtgtt gtgtccgtta ggtgcggtta atcaaatttt tgccggatgc
    15361 ggcgtgaacg ccttatccgg cctacaacgg gcaattttac ggcccccgtt aatctcaaac
    15421 tataccatta gcactggaag ttagcacgta caggtgggca aaaaaacgaa aactggtcaa
    15481 ccaaatatca gtttgattga ccagtaagtc tgtagggtaa tcagttagtt acagcgcagc
    15541 cacacactcg cgcgcaccgc gttcgcacag ctgctgatat gccgccgtca ccgcgccaac
    15601 aaagtcggca ttctgcggca gatcatcggc aaaaataccg ctcaggccca gcagcgcttt
    15661 cacgcggtct gcgccctgat actgcgcgtt aatcttctgg aactcggcca gcatcgggtc
    15721 gaccacgtca atggcattac cctgctcatc cacgccctgg gtgtaacgca tccagccagc
    15781 cacgcccagc gccaggtgac gccagctgcc gccattttgc aggtgcagac gcaccgggtc
    15841 cagcagacgc tgcggtaact tctggctgcc gtccatcgca atctgccagg tacggtgacg
    15901 cagagacggg ttgctgaaac gctagatcag cagcgtcgca taggccttca ggtccgtacc
    15961 ctccggcata gacagcgttg gcgcttgttc ctgcatcatc agggcaaagg ccgctttgcg
    16021 ataatccggg ttagtcatgg tgtcggcaat agtttcatag ccgccgaggt aaccgaggta
    16081 tgccaggaaa gagtggctgc cgttcagcat acgcagcttc atcatttcga acggcacaac
    16141 gtctgcaacg aactgtgcgc ccactttatc ccagtccggg cgaccattaa cgaagttatc
    16201 ttcaatcacc cactgacgga acggttcgca ggcaatggcg cacgggtcgt aaacacccag
    16261 ctggtcagca atttcctgta aggtttctgg cgtcgccgcc ggaacgatgc ggtcaaccat
    16321 ggtgcacggg aaggtgacgt tctcttcaat ccatgccgcc agctgcgggt cacgtgcctg
    16381 agccagcccc agtaccgcga ccttcgccac atgaccgttt tcacgcacgt tatcgcagga
    16441 catcaccgta aatgctttca gccctttttc acgacgcaga cgcaaggctt cgacgatgta
    16501 accaatcgcg gacttcggcg cagtcgggtt ttctagatcg tgcttgatca gcgggttatt
    16561 gagatccagt tgaccgcttg ccgcatcagc gcagtagcct ttttccgtga ccgttagaga
    16621 gacaatcgcc gtttgcggac gcgccatcgc gttgagaata ccttcgcagc cgtcgatttc
    16681 cggatgcagc gcttctttca tcgaaccgat aattttcagc tcggtgcttt ctgcgccttt
    16741 ttccgctacg gtgtacagca gttgctgttt tttcaggttt tcgatcagca cgcggtcgtt
    16801 gcctggcatc aggttaactt cgcagatgcc ccagtcgctg tcggtgcttt ccagcagatg
    16861 atgggtatac agcgcctgat gtgcgcggtg aaacgccccg caaccgagat gcacaatgcg
    16921 tgattccaga cgagaatgat cccatgacgg acgggcaacc ggcagattgc tgtcaacaat
    16981 agtagtcatc gtaaactcca gaatccgtct tcagagagtg acggcaagga agaaggaacc
    17041 ggcacggcgc gcagccatgc cggtggatat taacggctaa agaaagcgcg ctggatcgcc
    17101 agttcgacac cgcgaacttc ggccaggcct ttcagacgac caattgcgga gtaacctggg
    17161 ttggttttct tcttcaggtc gtccagcatc tgatgaccgt ggtccggacg catcgggatc
    17221 aggtcttctt tgccttccgc tttacgacgg tgttcttctt caacaatcgc tttcaccact
    17281 tcgtacatat caacgtcacc gttcaggtgc gccgcttcgt ggaaggtttt cgggttatct
    17341 tcacgcatgg tggagcgcag atgggtgaag taaatacgcg gaccgaactg cttgatcata
    17401 tcaaccagat cgttgtcagc acgcacgccg taggaaccgg tgcacatggt gaaaccgttc
    17461 gccatgctgt ttacggtatc aaccatccac tgcatatctt caatggtgga aacaatgcgc
    17521 ggcaggccga ggatcgggcg cggcggatcg tccgggtgaa ccgccatacg cacgccaact
    17581 tcttcagcaa ctggaataat cgctttcagg aagacggcaa agttttcgcg cagcttcgct
    17641 ttatcgatat ctttgtacag ctccaggtgc ttacggaact ggtcgagggt atacccttct
    17701 tccgcgcccg gcagaccggc aatgatgtta cgcgtcagac gcgctttgtc ttcatcgctc
    17761 atagtggcga agcgttcagc tgcctgagca atttcttctt cggtgtaatc cgcttccgcg
    17821 cctggacgtt tcaggatatg catttcgaat gcagcgaatt cgatctggtc gaagcgcaga
    17881 gctttggagc cgtctggcag cacgtattcg aggtcagtac gggtccagtc gagcaccggc
    17941 atgaagttgt agcacacggt gcgaatgccg cactgcgcca ggttgcgcag agtctgctga
    18001 tagttagcga tccactgctc atagttgcca gtgtgggttt tgatatcttc gtgaattggt
    18061 acgctttcta cgacagacca caccaggcct gcgtcttcaa cgatcgcctt gcgtttgagg
    18121 atctcttcta cggaccatac ttcgccgttc gggatatggt gcagcgcggt aaccacgcca
    18181 gttgcgcccg cctgacggac atcagctaaa gaaaccagat cgtttgggcc gtaccagcgc
    18241 caggtctgtt ccatgatttt tcctctttca catagtgggt tccacagcaa agagacgcgg
    18301 aaccctggag ttgtttaggt atccggatga acaaccagac cgcagccgtg gcgttgcatc
    18361 ggatgagatg gcgtataagt tctacctttt ggtaagatat atcaacccaa atcctgaaat
    18421 tggttaacca aatcacaaga atttcactct catggttgtt gctgcaaatt gcagcaaaag
    18481 gtaatcgatg gttattgatc agaggatgtg acattcatcg caacaatggt tgaccaattt
    18541 acataacatg tcgcccaaaa taacaacggt tcaaccaggt aaggtaaaac gttatgcatg
    18601 tgcttaacat tctctgggta gtattcggca ttggtctgat gctggtactg acaacgaccg
    18661 caggttcaga cacattcact gcattagcaa tgcctcttag attattttga cctgtcagta
    18721 aaagacacgt tgaaaacctg gggtctgctg gaactggtca actccgtggt tgggctgatt
    18781 attagcaagg tagcgtaaaa gaacaccaga gcctgccaat ggcaggctct gactgatgac
    18841 aaagtctaaa aatgtgcccg gacagtcccc tcgcccctcc ggggagaggg ttagggtgag
    18901 gggaacagac cagcaaagtg cgctttgttt atgccagatg cgacgctgac gcgtcttatc
    18961 tggcctacaa agggctaacg tgcaggtttt tagcttcagg taatattgcg taactgcatt
    19021 agcaatgccc tgtgatttct ttattgataa acaaaagtca cgccaataat cgattgcaca
    19081 ttccctgcag tcacctgccc tccggtacgt gcgtaatttg ccgttaatcc cagacttacc
    19141 gccgaagttc
//
LOCUS       DADLFA010000070        19134 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000070 DADLFA010000000
VERSION     DADLFA010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19134
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.070"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(31..2478)
                     /gene="glgP"
                     /locus_tag="JQH65_002060"
     CDS             complement(31..2478)
                     /gene="glgP"
                     /locus_tag="JQH65_002060"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HAY6139471.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2497..3930)
                     /gene="glgA"
                     /locus_tag="JQH65_002061"
     CDS             complement(2497..3930)
                     /gene="glgA"
                     /locus_tag="JQH65_002061"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HAY6139472.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRASLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3930..5225)
                     /gene="glgC"
                     /locus_tag="JQH65_002062"
     CDS             complement(3930..5225)
                     /gene="glgC"
                     /locus_tag="JQH65_002062"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HAY6139473.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLKAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5243..7216)
                     /gene="glgX"
                     /locus_tag="JQH65_002063"
     CDS             complement(5243..7216)
                     /gene="glgX"
                     /locus_tag="JQH65_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HAY6139474.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWDSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(7213..9399)
                     /gene="glgB"
                     /locus_tag="JQH65_002064"
     CDS             complement(7213..9399)
                     /gene="glgB"
                     /locus_tag="JQH65_002064"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HAY6139475.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDASISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9672..10775)
                     /gene="asd"
                     /locus_tag="JQH65_002065"
     CDS             complement(9672..10775)
                     /gene="asd"
                     /locus_tag="JQH65_002065"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAY6139476.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10967..11560
                     /gene="yhgN"
                     /locus_tag="JQH65_002066"
     CDS             10967..11560
                     /gene="yhgN"
                     /locus_tag="JQH65_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HAY6139477.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11617..12957)
                     /gene="gntU"
                     /locus_tag="JQH65_002067"
     CDS             complement(11617..12957)
                     /gene="gntU"
                     /locus_tag="JQH65_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HAY6139478.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGPLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12961..13488)
                     /gene="gntK"
                     /locus_tag="JQH65_002068"
     CDS             complement(12961..13488)
                     /gene="gntK"
                     /locus_tag="JQH65_002068"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAY6139479.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMLRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13627..14622)
                     /gene="gntR"
                     /locus_tag="JQH65_002069"
     CDS             complement(13627..14622)
                     /gene="gntR"
                     /locus_tag="JQH65_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HAY6139480.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVSYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14846..15541)
                     /locus_tag="JQH65_002070"
     CDS             complement(14846..15541)
                     /locus_tag="JQH65_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HAY6139481.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15664..16701)
                     /locus_tag="JQH65_002071"
     CDS             complement(15664..16701)
                     /locus_tag="JQH65_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY6139482.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFISDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLSVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYDLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     PEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17033..17521
                     /gene="yhhY"
                     /locus_tag="JQH65_002072"
     CDS             17033..17521
                     /gene="yhhY"
                     /locus_tag="JQH65_002072"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY6139483.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            17758..18957
                     /gene="hcp"
                     /locus_tag="JQH65_002073"
     CDS             17758..18957
                     /gene="hcp"
                     /locus_tag="JQH65_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HAY6139484.1"
                     /translation="MSNIVYLTVTGEQQGSISAGCGTSESTGNRWQSGHEDEIFTFSL
                     LNNINNTGLGSQFHGITFCKLIDKSTPLFINSINNNEQLFMGFDFYRINRFGRLEKYY
                     YIQLRGAFLSAIHHQIIENQLDTETITISYEFILCQHLIANTEFSYLALPENYNRLFL
                     PNSKNQTNNRFKTLNSKAIGRLLAAGGVYNGNIEGFRDTAEKLGGDAIKGYDQILNEK
                     TAGIAIATASILLTKRSNVDTYTEINSYLGKLRGQQKLLDGIDIIEIIYIKRPSKDLA
                     NLRKEFNKTVRKNFLIKLAKTSEASGRFNAEDLLRMRKGNVPLNYNVHHKLSLDDGGT
                     NDFENLVLIENEPYHKVFTNMQSRIAKGILVGESKITPWAIPSGSIYPPMKNVMNASH
                     TIKHGAV"
BASE COUNT         4903 a         5062 c         4651 g         4518 t
ORIGIN      
        1 cctttggccc cgttctattt attggtgaac ttacaatctc accggatcga tatgccagat
       61 atgatcggcg tactctttga tagtacggtc agaagagaag tagcccatat tggcaatgtt
      121 cagcatcgct tttgcggtcc actcttcctg aagctcgtag agttcgtcga ctttatcctg
      181 acaatcgaca tagctgcgat aatccgccag aacctgatag tgatcgccga agttgatcag
      241 cgaatcaacc agatcgcgat agcgacccgg atcttccgga ctgaatacac cgctgccgat
      301 ttgcgtcagc acctgatgca gctcctcatc tttctcgtag tattcacgcg gtttgtagcc
      361 ctgacgacgc agttcttcca cttcttccgc tgtgttacca aaaataaaga tattgtcagc
      421 accgacatga tccagcatct cgacattcgc accgtccaac gtaccgatag tcagcgcacc
      481 gttaagcgca aacttcatgt tactggtgcc ggaagcttcc gtccctgcca gcgaaatctg
      541 ttcagacaga tctgccgccg gaatgatcaa ctgcgccagg ctaacgctgt agttcgggat
      601 gaacacgact ttcagcttat cgccaatctg cggatcgttg ttgatcactt tcgctacgtc
      661 attgatcaaa tgaataatgt gcttcgccat gtaataggcc gaagccgcct taccgccaaa
      721 aatattcacg cgcggtaccc acttcgcatc cgggtcggcc ttgatgcggt tatagcgggt
      781 aatcacatgc aacacattca tcaattgacg tttgtattcg tgaatacgtt tgatttgtac
      841 atcgaacaac gcctttggat tcaccaccac attcagctgc tgggcgatat actctgccag
      901 acgctttttg ttctccagct tcgcctgatg cacagcgtga ttaaccattg ggaaatcaca
      961 gtgttgttgc agctcattaa gcaggctaag gtcggtgcgc cagttacggc ccaggtgttc
     1021 gtccagcacg gctgaaagcg atgggttcgc taccgctagc cagcgacgcg gcgtcacacc
     1081 gttggtgacg ttggtgaaac gacccgggaa gattttcgca aagtcggcaa acaacgattg
     1141 caccatcaga ttagagtgca gttccgatac accgttaact ttgtggctca caacaaccgc
     1201 cagccaggcc atacgcacac gacgaccgtt ggattcatca atgatcgacg cccgtcccag
     1261 cagatcggta tcgttcggat actgttcctg caaggttttc aggaaatagt cgttgatttc
     1321 aaagatgatc tgcaggtgac gcggcagaat tttacccagc atatcaaccg gccaggtttc
     1381 cagcgcctcg ctcatcagcg tgtggttagt gtaggagaag acctgacaac acacctcaaa
     1441 cgcgtcgtcc cagctaaatt ggtgctcatc gatcagcaga cgcatcatct caggaatcga
     1501 cagtaccgga tgggtatcat tgagatgaat cgcgatttta tccgccaggt tatcgtaggt
     1561 tttatgcaac tgataatggc ggcttaaaat gtcctgaatg gtcgaggaaa ccagaaagta
     1621 ttcctgacgc aggcgcagct cacgcccgga gtaggtggag tcatccggat acagtacgcg
     1681 agatacgttc tcggagtggt ttttatcttc cactgccgcg aagtagtcac cttggttgaa
     1741 tttaccgagg ttaatttcgc tactggcttg cgcactccac aaacgcaacg tgttggtcgc
     1801 gtcggtgtcg taaccaggga ttatctgatc gtaagcgact cccagaatct cttcggtttc
     1861 aatccagcgc gtttttttac cttcctgctg aatgcgacca ccaaaacgga ctttatagcg
     1921 cgtgttgtgg cgtttgaatt cccacgggtt accgtattcc agccagtagt ctggcgactc
     1981 tttctggcta ccgttaacga tgttctgctt gaacataccg tagtcatagc ggatgccgta
     2041 accgcgcccc ggcaacccta acgtcgccag agaatcaagg aagcaagccg ccagacgtcc
     2101 caggccaccg ttaccgaggc ctgggtcatt ttcttcatca atcagctctt cgagatttaa
     2161 ccccatcgct tccagtgcgc cctgtacatc ttcgtaaatt cctagcgaca acatggcgtt
     2221 ggagagcgta cggccaatca aaaactccat cgacaggtag taaacctgac gagtttcttg
     2281 cgacaactgg gcacggtttg aacgtaacca gcgctccacg agacgatcgc gcacagcaaa
     2341 taacgttgcg ttcagccatt catgtttatt ggcgacgacc gggtcctttc caatcgtaaa
     2401 catcagcttg taagcgatag agtgcttaag agcttctacg ctaagcgtgg gcgatgaata
     2461 tgtaaacgga gcattcatat aggcgtttcc tgaaaactat ttcaagcgat agtaaagctc
     2521 acggtacgac ttcgccgcga cctgccagct aaaatccatt gccatagcct gacgttgcac
     2581 aaaccgccac agtgaagcac gagaccacag tacaaaagca cgtcgaatag cccgtaacag
     2641 cgaccaggca ttactatctt caaagacaaa cccactggcg acgccatctg caaggttctc
     2701 aagagaacag tcagaaaccg tatcagcaag cccaccggtg cgccgcacta acggcagcgt
     2761 accgtacttc aatccataaa gttgcgttaa gccgcacggt tcaaaacggc tgggcaccag
     2821 aatgacgtcc gcgccgccca taatgcgatg cgaaaatgct tcgtgatagc caatctgaac
     2881 gcccacctgg ccggggtatt ccgctgccgc cgcaaggaaa ccttcctgca gcaccggatc
     2941 gcccgcgccg agtagcgcca gctgcccgcc ctgctccaga agacccggta aggcttccag
     3001 caccaggtcg agacctttct ggctggtcag acggctcacc actgcaaaaa gcggcacttt
     3061 atcgtcaacc ttaagcccca ttgcgatttg taactggcgc ttattttccg ctttatcttc
     3121 caacgtatcg cgggtgtaac gcgaggccaa cagtaagtcc gtctctggac tccagatttt
     3181 ctcgtccacg ccgttcagta cgccggaaag acgcccttca cggtgacgct gttgcaacag
     3241 accttccata ccgtaggcaa actgcggttc ggtgatctcg cgagcgtagg ttggactgac
     3301 cgccgtaatg tgatcggcat agtacagacc ggccttcagg aaagagattt gtccgttgaa
     3361 ttccagccca tgaatattaa agaatgacca tggcaattgg atgtcattca tgtgatgtgc
     3421 ataaaacatg ccttgatagg ccaagttgtg cacagtaaac accgacttcg ccggacgccc
     3481 gcgcgccgcc agatacgcag gcgcaaggcc tgcatgccag tcgtgcgcat gcaccacatc
     3541 aggacgccag aatgggtcaa gcccgctggc catttctgcc ccaacccacc ccagcagcgc
     3601 aaaacgcaat acgttgtcgg tataggcaaa taagttggta tcgtgatacg ggcttcccgg
     3661 acgatcatag agatgcggcg cgtcaatcag gtatatgcca accccgttgt aatgaccgaa
     3721 caacagcgtg atatgtccgg cgaaggtatc acgacgggat actacctgcg catcggtcac
     3781 gccacggcga atatcgggaa atgcaggcaa cagtacgcga gcgtcaacgc cgtctgcgat
     3841 ttgtgctgcg ggtaatgccc caataacatc agccagaccg ccggttttaa gcagcgggaa
     3901 catctctgaa catacatgta aaacttgcat tatcgctcct gtttatgccc taacttccgt
     3961 agcatttcgc gcgttaccag cacgatgcct tcttctgaac gatagaaacg acgtgcatct
     4021 tcctctgcgt tttcaccaat caccatgcct tccgggataa cacaagcacg atcgatgacg
     4081 cagcggcgca gacggcacga gcgacctacc catacttccg gtaacaatac ggcggaatca
     4141 atgttgcaga atgaattcac gcgaacgcgc gagaacagaa cggactgcac caccaccgaa
     4201 ccggagatca cacaaccgcc ggataccagt gagttaaggg tcatcccgtg gctaccggag
     4261 cgatcctgca cgaatttcgc tggcggtaat gattcattgt aggtgcgaat tggccaattg
     4321 cgatcgtaca tatccagttc cggcaccaca gaggccagat cgagattcgc tttccagtaa
     4381 gctttcagcg tacccacatc gcgccagtac ggctcggcat ccgggtcgga ttgtacgcaa
     4441 gagagcggga acgggtgcgc ataggccaga ccggcttcgg tgatcttggg aatcaaatct
     4501 ttgccaaagt cgtggctgga gttctcatca cgatcgtctt cttccagcag ttcatacaga
     4561 tagtcggcgt caaagacgta gatacccata ctcgccagag atttgctcgg atcgttcggc
     4621 attgacggtg ggttagcagg tttttcaacg aattcgataa ttttatcgtt ctcatcaacc
     4681 gccataacgc caaatgcgga ggcttcttca atcggtactg gcatacaagc aacggtgcaa
     4741 cgtgcgcctt tttcgacgtg atcgataagc atacgcgagt agtcttgctt gtagatatgg
     4801 tcgcccgcca ggatcaccac gtattccgct ttataacggc ggataatgtc gaggttttgg
     4861 gtgaccgcat ctgcggtgcc gcgataccag ttttcccctt tcattctctg ctgtgctggc
     4921 agcagatcga caaactcgtt catttcttca ttgaagaatg accagccgcg ctgaatgtgc
     4981 tgcaccagag tgtgggactg gtactgggtg atcacgccca tacgacggat cccggagttg
     5041 atgcagttag acagcgcaaa gtcgataatg cggaacttac cgccgaagtg tacggccggt
     5101 tttgctcgct tattggttaa atccttcagg cgggtaccac gtcctcccgc cagtatcagg
     5161 gcaacagatt tcaatggcag ctggcgcgcc aacattaagt gatcgttctt ctctaaacta
     5221 accatgacta actccttttt tatcatctct ggaacacaca caatccgtgt gcaggtccct
     5281 gccagacagc cgtaatcact gggttatcct ctccagcgaa tgggggaatg gcgtgccact
     5341 ccccagcagg taaaacaatc tctgttacct caagcgtggc gttaattgcg atcaaaaagc
     5401 gatccgagag cagaatttgc agctgtttcg gcccgttttg ccactcatcc gtgcttaaag
     5461 gttgagcata tcgatttagc caacggacat tgccatcgcc ttcttcccac cagcgattct
     5521 ccaccaaagc gggaatgcgc ttgcgcagat ggattaacgc ggcggtaaat gcggttaaac
     5581 cactgcttgc ctgcgaccag tccaaccagg ttaattggtt atcctgacag taggcattgt
     5641 tattgccatg ctggctgtga ccatgttcgt caccggccag taacatcggc gtaccctggg
     5701 agagcaacaa cgttgttaac aaggcgtgaa tactgtcgcg ccgccgttca accaggtcaa
     5761 gagaaccgcc taacccttct ttaccatgat tgttactgta attgttgttg gtcccgtcgc
     5821 gattttcttc tccgtttgct tcattgtgtt tatggttgaa gcaaacgcag tcgcgaagcg
     5881 taaaaccgtc atgcgcagtg acgagattaa tcgcggcact cggcagacga ccattacgtt
     5941 taaaaacatc gctggaggca gcaaaacgcc cggcaaacgc ccccagaggc aaatcataat
     6001 gtagccagaa acgacgggca gcatcgcgga aatgatcgtt ccactcggca aacagcggcg
     6061 ggaaatttcc cacctgataa ccaccagggg cgatatccca cggttcagca attaacttca
     6121 cctgcgagag caccgggcag ttctggatag cggtaaacaa cggcgcatcc tgacggaact
     6181 ctggcgtacg gcccatgact gccgccagat caaagcggaa accatcgacg tgacaggttt
     6241 ctacccaata acgcaggcag gcgctggcat aatccaccac cgccggatga ctcaaattga
     6301 gcgtgttgcc gcaaccggtc cagttgtgat aatcgccgtc ttctcttatc caataatagc
     6361 tacggttatc gatcccacgc agtgagaata acgggccgtc gaggtccagt tccgcactat
     6421 ggttgagcac gatatcaaga atgacttcga tacccgcttt atgcagtgct ttgattgcat
     6481 cgcgaaactc atccagcgcc gtttctggcg agcaggcata cgccggatgc agcgcaaaca
     6541 tcgccaccgg gttgtaaccc cagtagttac ttagccccat gcgttgcaga cgtggttcac
     6601 tggcaaactg cgccactggc agcagttcca gcgcggtaat gcccaattgt ttcaaatagt
     6661 tgatcatcac cggatgcccg agggctttat aagtgccacg gatctcgacc ggaatctccg
     6721 ggtgcaagta cgttaatcct ttgacatggg cttcataaat gatggtgctg tcccacggcg
     6781 tgcgcggcgg ggcatcatct tcccagtcat agtgatcaac caccactacg catttcggcg
     6841 caatggcggc gttgtcgcga tagtcaggtt cattatgacc ggcgtgcagc agcgggttat
     6901 ctttaaattc cccgtcaatt tgccgcgcgc aaggatcaat caacaacttc gccgggttaa
     6961 agcgatgccc ctcggcgggt tgccaggggc catgaacgcg ataaccataa cgcaaacccg
     7021 ggcgcgcatc cggcagataa ccgtgccaaa tgtcgccact gtgccctggc aagtcatagc
     7081 gatgttcctg gccattggcg tcaaagacac acagttctac ccgctcggca tgagcggaga
     7141 aaagtgtgaa gttgacgccc tgaccgtcgt aatgcgcgcc gaggggagcg ggtttgccaa
     7201 tggcgagttg tgtcattctg cctcccgaac cagccagata gtggccagcg gtggtagtgt
     7261 caggcttagt gaatgctgac gaccgtggct ggcaatctca tcgctgtgta ccgtgccgcc
     7321 attgcctgca ttactgccgt gatagtgcat ggaatcggta ttgaggattt cacgccattt
     7381 gcctggctga ttaatgccga agcgataatc atgacgcggc accggcgtaa agttactggc
     7441 aacgataatt tcgttaccct ctttatcgcg acgcacaaag atcagcaccg agcgttcttt
     7501 gtcatccacc accagccatt caaagccgta cgggtcaaaa tccagttcat gcattgcttt
     7561 atggtggcgg taggtgaggt tcagatcgcg caccagacgc tggacaccgt ggtgccagtt
     7621 atcgccacct tccaacagat gccagtcgag gctggcgtca tggttccact cacggccctg
     7681 ggcaaattcg ttccccatga acagcagttt cttgcccggg aatgcccaca tccagccata
     7741 gtaggcgcgc aggttagcga atttctgcca tgcgtcgccc ggcatgcggt cgagaatcga
     7801 ttttttaccg tggaccactt catcatgcga caacggcagg acgaagtttt cagtgtagtt
     7861 gtagagaatc ccgaaggtga gtttatcgtg atgatactga cgataaaccg ggtcgagctt
     7921 catgtagtcc agggtgtcat gcatccagcc gaggttccac ttgtaccaga agcccagacc
     7981 gcccatatcc tgcggacgag aaacgccagg gaaatcggta gactcctcag ccattgtcac
     8041 cgcaccggaa acctgctcac caagaatacg gttggtatta cgcaagaatt caatcgcttc
     8101 aagattctcg cgcccgccaa attcgttcgg gatccactcc ccctctttac ggctgtagtc
     8161 gcgataaatc attgacgcca ccgcatcgac gcgcagcgca tcaataccaa aacgttcaat
     8221 ccagtaaagc gcgttaccga cgaggaagtt actgacttca cggcgaccat aattgtagat
     8281 cagcgtattc cagtcctgat gatagccttc gcgcggatcg ctgtgttcat acaagttcgt
     8341 gccatcaaat tcggcaagcg caaagtcatc ggtcgggaag tggcctggca cccagtcgag
     8401 aatcacgttc agacccgctg cgtgtgcggc atcaatgaaa taacggaagt cgtcgcgagt
     8461 accaaaacgg cgggttggtg catacaggcc ggttggctga taaccccaac tgccatcgaa
     8521 gggatgctcg ttaatgggca gtagttcgag gtgggtaaag cccatccatt tagcataagg
     8581 caccagttga tcggccagct cgcggtagct caaccagaaa ttgttatcgg tgtggcgacg
     8641 ccaggagccc aggtgaactt cataaataga gattgacgca tcaaactgat tcgctttttt
     8701 gcgctcttca gtctgtacaa ccttttccgg cagcccgcaa ataagagacg cggtttccgg
     8761 gcgcatttgc gcttcgaagg cataagggtc ggacttcaga cgcaagttgc cattggcatc
     8821 aatcatctcg tatttataga gctgaccgtt atgcgcccca gggataaaca gttcccaaat
     8881 gccgctctct ttacgcagac gcatcgggtg acggcgaccg tcccagtagt tgaattgccc
     8941 aaccaccgag acccgacggg cgtttggagc ccaaacagag aaacgcgtac ctgtgacgcc
     9001 atccatagta tctgcatgcg cgcctaaggt ttcatacggg cgcaggtgag taccttcaga
     9061 taatagccag gcatccattt cctggattag cgggccaaaa cggtaaggat catcaatcag
     9121 gttttgctga ccatgccaga caacagccaa ctgatagcgg aaaaaattct tacgtcgcgg
     9181 aatgacaccg ctaaagaatc cccgtgagtc aagacactcc agttttgcga gtttgcgccc
     9241 ggttttcggt tcaatcaccc acacatcggt agcgtcgggt aaaagggcac ggacttccag
     9301 tcccgcggtg gttttatgca ttcccagtac ggaaaaagga tccgcaaaat ggcctgcaat
     9361 tagcgcgtta atcacgtctc tatcgatacg atcggacatg cttgtcttcc tgttttattg
     9421 tgtcacccca tccaattgga tttttgactt ctggttgtga catttttttg acctgaacgg
     9481 cgcagcactc tgtgcatcct ctctgcgtcg tcctcacttc aggtaaggct gtgaatactc
     9541 atgtattcag ccacccttaa agaatagcca atgctctatt taactcccgg taaatcatga
     9601 aacatctgcg cttactcctg tattacgcac taacaggggc ggcatcgcgc cccagattta
     9661 atgaataaag attacgccag ttgacgaagc atccgacgca gcggctccgc ggccccccac
     9721 agcagctggt cacccacggt aaaggctgac aggaactccg gtcccatatt cagcttacgc
     9781 aggcggccta ccggcgtggt cagcgtgccg gtaacggcag ctggggttag ctcacgcata
     9841 gtgatttccc gatcgttcgg aacgactttc gcccacggat tgtgcgcagc cagcagttct
     9901 tcgacggtcg gaatagacac atcttttttc aatttaatag tgaatgcctg gctgtggcag
     9961 cgcaatgccc cgacacgcac acataaacca tctaccggaa ttacggaaga tgtgttgagg
    10021 atcttgttgg tttccgcctg ccctttccac tcttcgcggc tctgaccgtt atcgagctgt
    10081 ttgtcgatcc acggaatcag gctacccgcc agcggcacgc caaagttatc caccggcagc
    10141 tcaccgctac gggttaaggt tgtgactttg cgttcgatat cgagaatagc agaggacggg
    10201 gtcgcgagtt catctgccac atggccatac agatggccca tctgggttaa taactcacgc
    10261 atatgtcgcg caccaccgcc ggaagcggcc tggtaggttg caacggacac ccaatcaaca
    10321 agatcattgg cgaataaacc acccaacgac atcaacatca ggcttacggt acagttaccg
    10381 ccaacaaaag tcctgatgcc attatttaat ccgtcggtaa tgacgtcctg attgacgggg
    10441 tcaagaatga tgatggcgtc atctttcatg cgcagagacg atgctgcgtc aatccagtaa
    10501 ccttgccatc cgctttcacg aagctttgga tagatttcgt tggtataatc gccgccctga
    10561 caggtcacaa tgatatcgag ggcctttagc gcctccagat caaaggcatc ctgaagtgtg
    10621 ccagtggttc cgccaaaaga cggcgcagcc tggccaagct gagaagtaga aaagaagaca
    10681 gggcgaatgg cgtcgaagtc gcgctcttca accatgcgtt gcatgagaac ggagccgacc
    10741 ataccgcgcc agccgataaa accaacattt ttcataagcg ttttttcctg caaagatgtg
    10801 tgctgtataa atgtgccggt ctcctcttgg cacatctttc accatacaaa aagcagccaa
    10861 agtcgcaagt gaaattaatc aatgatagcg aagccatcag taatgcgact tatcctgctt
    10921 tgttagcaca cagaaagtcc gcggcaatta tcagggaatt tgagttatga atgaaatcat
    10981 ttctgcagca gttttattga tcctgattat ggatccgctc ggaaacctgc ctattttcat
    11041 gtccgtactg aaacatactg aaccgaaaag acggcgggca atcatggtgc gagagttgct
    11101 tattgctctc ctggtgatgc tggtgttcct gtttgcgggt gagaaaattc tggcatttct
    11161 cagcctgcga gcagaaaccg tctccatttc tggcggcatc attctgtttc taatcgccat
    11221 taagatgatt ttccccagcg cttcaggaaa tagcagcggg cttccggcag gtgaagagcc
    11281 atttatcgtg ccgttggcaa ttccgttagt cgccgggccg actattctcg ccacgctgat
    11341 gttgttgtct catcagtacc cgaatcagat gggacatctg gtgattgctc tgctgctggc
    11401 ctggggcggc acctttgtca tcctgctaca gtcttcgcta tttttacgtc tgctgggcga
    11461 gaaaggggtg aacgcacttg aacgcctgat gggattgatt ctggtgatga tggcaaccca
    11521 gatgttcctc gacggcattc gaatgtggat gaaggggtaa cgaggagagt ttttcgaaaa
    11581 gaacccggta gcgtcacaac taccgggcga acaaacttaa ctcaacagct ggaacgcaat
    11641 catcccaacg atggcaccaa cagtgccgag gatggtttcc atcatggtcc aggttttcag
    11701 cgtttcggct tcggtcgcgc cggtaaattt accgaacaac cagaaaccgg cgtcgttaac
    11761 gtggctgaca acaatcgaac caccagcgat acaaatcgac agtgccgcca tttgcgcacc
    11821 ggagtagttc agttgttcaa tgaccggcat caccagcccc accgccgtta aacaggcaac
    11881 ggtggcagac ccctgaatga tgcgcactgc agcggccagc acgaagcagg tgatagcaat
    11941 cggcaggccc atgccggtta acgcttcgcc cagtgccgga cctacgccag agtcaaccag
    12001 cacctgtttg aacacgccgc ctgcaccaat caccagcaga atgatccccg ccggttgtag
    12061 cgcgtgaccg caaatctcca tcactttgtc ttttggcatg ccctgacgca ttgccaggcc
    12121 ataaatcgct accagacaag caaccagaat cgcggtaaat ggatgaccaa taaactcgaa
    12181 ccattcgtaa gcagttgatc cttctggcac aaaacgagcg gcaatggttt tcagccccac
    12241 cagcaccagc ggcaacagga tcagcgacag gctgaatccg aaggacggca ttttgccttc
    12301 gccgagatgc ggttcgctga tgtcgtcagg aatatgcaac tccacgtaac ggctgatgaa
    12361 attaccccac agcggcccgg caataatcat tcccggaatt gccgcacaca gaccaatcag
    12421 gatcatccag ccaaagtcgg cattcatctg cgatgccagc aacatcggcg ctggtccagg
    12481 caccagaaat gccgccgctg ccgccacgcc tgcgaataat gggattacca gcttcaccag
    12541 gttcgtaccg gtgtggcgcg ccattgagaa agcaacgcta atcagcagaa ctatcgccac
    12601 ttcaaagaac agcggtagcg cacagaccaa ccccgcaagg ccgatggcat aatgcgcgcg
    12661 gctgtgaccg aaggatttga gcattttgac ggcaatctga tcgactgcgc cggtttcatg
    12721 taagatcttg ccaaacatag ctcccagggc gacaaccacc gccaggaagc cgagggggcc
    12781 tcccatccct ttttccatcg tcgctgcgat tttatcgagc ggcataccgg aaaaaaggcc
    12841 agcccccatg gacaccacca tcaaagccag gaaagcgtgc atacgcgcct tcatgactaa
    12901 aaacagcagc agtaaaacag accctactgc tgttaaaaca agcgttaatg tagtcactac
    12961 ttatttgcct tttttaataa cctcaatggt gcttgccaca acaccttcca gcggttgatc
    13021 gatatccacc accagtacat cggtttcgtc cgcacccggc tcctgcagcg tttcaaactg
    13081 cgtcaccaac atttgggttt taaagaaatg gcctttgcgc gctttcaagc gactttcaat
    13141 cacatcaaaa tcgcctttca ggtagatgaa agagagattc ggattacctt cacgcagcaa
    13201 gtcgcgatag tgctttttca gtgcagaaca gacgatcagc gacactttat tggtgcgcag
    13261 catagcaaac gcggcgtcgt tcagcgcctg caaccagggt ttgcgatcgt cgtcattcag
    13321 cggttcgcca gacgccattt tttcgatatt gcgccgtgga tggaggaaat caccatcaag
    13381 aaacgcggca tgaagttgat gcgccacttc actggcgacc gcagatttgc cgctgcccga
    13441 tacgcccatc aagacgtaaa tgtggtgatc atggttagtc gtgctcaaag tggtgccccc
    13501 acaatacaag aattaaaaat gttacgggta actgttatcg gtaacattgt ccagccggac
    13561 aatatcagaa gcaatatcca tccgtgcctt aagtgtataa gtgtgagcta cttcaaattt
    13621 gtaggcttaa atagatccgc ccggtgacaa ggtgaaacct aaatctaaca ttttcggtgt
    13681 cacagattcg ccacgaatac gcgccagcag gcgttcagcg ccaatactgc ccatccgctc
    13741 acgcggcgtc agcacgctcg ctaaccgcgg ctccatcacc tggccaatgt catgaccgtg
    13801 gaaaccggca atcgccatat cgtcaggaac cttcaatccc agacgctgac attcaaacgc
    13861 cgcgccgacc gccaggtcat cgttggtaca gaacacgcca tccagctgcg gatattcccg
    13921 ccgcgcctgg cgaatcagtt caataccgga agagtaagaa gaagattgct caaccatcac
    13981 gctatacgac accagacctg catccagcat cgcctgttcg tatcccttct gtttgatgat
    14041 agtacgttcg tcgagacgcg cgccgagata ggcaatgtgg caatgcccgc gagcaataat
    14101 ggcggtggtc atctggcgtg ccgcttcaaa gttatcaaaa ccgacggcga tatcgaggca
    14161 cggcgactgg ctgtccatga gttccaccac cgggatcccc gccacttcaa tcatctttaa
    14221 ggtgcgcggc gtgtgggtac gttcggtgag gatcagtccg tcgatattcc atgagagcat
    14281 ggattcgaga cgttcttgct ccatttccgg tttataaccg tagtgagcca gcatggtctg
    14341 ataaccgtgc gcgtcggtga cgctttcgat tccgcgtaat acttccgcga aaacctggtt
    14401 ggtgagagaa ggtaacagga cgccaatcgc ccggctggtg gcgttagaga ggatatcggg
    14461 cgcacgattg ggaatatagc ccagttcatc aagagcggcg gcaatcttgc cgcgtagagc
    14521 gacggaaacc tgctccgggt tgcgtaaaaa acggctgacc gtcattttgg tcacgcctac
    14581 acggtcagcc acatcctgaa gtacgggtct tttctttttc atcgtcctga aggtacaaaa
    14641 gagatagatt ctcttagttt aacacggacg catcagaacc ttcccgggga aaacagtgct
    14701 tgtttacaat tattagatat acactaaatc aattgcatcg cattgtgcta acgacgcggc
    14761 aaaaccccgg aagcgtgcat cagcacgcga ccggagcgag cgaggaagcc tgcaaaaagg
    14821 caggcttcaa agatgccgtt gagttttaaa ccggcggcag atcgaacagt aacacttcgc
    14881 tgtcgctatc cgcatggatg gagattgcct gctcatccca gattgccaga ccatcgctgg
    14941 tcgaggcttt cacgccgtta atggtgacat tgcctttcac cacctggatc cagacgcggc
    15001 gttcagcggc aatctgatgc accgactgct catctttcag caacgcccag cggtacagtt
    15061 ccatatcctg atgcactttc aatgaaccat ctcgcgcatc cggcgagagc accagctgtt
    15121 tgccctgtac ggcatcgaag cgacgctgtt cataacgcgg cgtaataccg ttttcttcag
    15181 gcatgatcca gatctgatac agatgcagac gctcggtgct gcttgggttg tactctgagt
    15241 gacgaatacc cgtacccgca ctcataatct ggaactcacc cgccggaacc tgctctttat
    15301 tgcccatgct gtcctgatgc tcaacagtac cttccagcac gtaggtcaaa atttccatat
    15361 ctttatgcgg gtgagtgccg aagccctgcc ctgcttcaat cacgtcgtcg ttaatcacgc
    15421 gcagcgcgga gaagcccata aagttcggat cgtaatagtt ggcaaaagag aaagtatgcc
    15481 aggagtccag ccagccatga tttgcatgac cacgttcatt tgctttgcgt aagtagatca
    15541 tttgtattcg ccccctgaat gatttcgatg ggcttagtgt ggacccgatc tgcctgggat
    15601 gatagagggt gaaaattgcc ccctctgttc aaaaaaattg aactattcga ggggccatca
    15661 aacttaattc gcgagagtca ctgtggaggg agaggcttgc tcaaatccgc gttcaaggat
    15721 ttccagattg gtaagaactt cagattcctt gacgtaattt ggcgcaccgt gggtgatggt
    15781 ttgatacaac gcatcataaa cgcgcccgta atcgcccatc tccggcttca tctcttctct
    15841 gaccgtcacg ccctcgtcat tgacatactc cggcacaccg accgaatcat ccgctgcgaa
    15901 tcccggttcg cccggcataa tattagcctt caggctggtt tcctgctggt cgataccgta
    15961 tttaataaac gaacctttct taccgtgaac gataaatttc ggataatcga ttttcaccag
    16021 atggctggtt ttgacgatgg ctttcaggtc gccataaaac agttgcgctt caaaggtgtc
    16081 gtcaggattg gctttattac gcaggctgcg gatgtcataa gcgacgtgat ccgggcgacc
    16141 gaacagagaa ataatctggt ccatcgtatg cacaccaagg tcataaaacg cgccatcctg
    16201 cggcagccca ggtttggttt ctgccaccgg gcggtaatag tcaaaatggc tttccacttc
    16261 aacaatctct cccaacttgc cactttcaat cgcttttttc gctgtcagga agcaggagtc
    16321 aaagcgacga ttctgatacg gcgtgacgct cagccctttg cttttcgcca acgcaaacag
    16381 ctccttcgcc tgggcaagcg tcggggtgaa cggtttttcg accagcacat ttttcccggc
    16441 ttccagcgcg cgtttcgcgt actcgaaatg gctgtccgcg tgggtgcaga caacaaccag
    16501 cttaacatcg ggatcgttta gtacttcgtc gagatcgctg atgaaatgga tatgggaata
    16561 aatgggagcc tgttcttccg gcttcgcatg gcgacgaaaa atatgcgcga catgccagct
    16621 atccttgcgg ttaagtacat acggcagatg gtaacgggtg gtgcttttgc cgaagccaat
    16681 aaaggcgcag ttgatgacca tgatttcgtc ctttttaagg tggttattta caccttagcg
    16741 caaagcggac gtggttccta ctggagtgcg cataacgaac acaagcactc ccgtggataa
    16801 attgagaacg aaagatcaaa aaaaagccag cacccggctg gctaagtaat actggaagca
    16861 atgtgagcaa tgtcgtgctt tcaggttctc cgcgagggtc ttcctgatcg cgagacaata
    16921 ataatcattc tcattcgcac ttgtccaaca ctttttgcaa aaaaatgcat ttgactcgca
    16981 tttgaaagtc aatgatgttg aaagggacat ttaccccaaa gaggacaaag gaatgagtga
    17041 gatagtaata cgccacgcag aaacacggga ttacgaggcc atcaggcaga ttcacgccca
    17101 gccggaggtg tattgcaaca cactacaggt gcctcatcct tccgatcata tgtggcagga
    17161 gcgactcgcc gatcgtcccg gcatcaagca actcgtcgcc tgtattgatg gagacgtcgt
    17221 gggccatctc accattgacg tgcaacagcg cccacgccgc agtcatgttg ccgattttgg
    17281 tatctgtgtc gactctcgtt ggaagaaccg cggcgtcgcc agcgccctga tgcgagagat
    17341 gattgaaatg tgcgacaact ggttgcgggt agatcgcatt gaactaaccg tgtttgtcga
    17401 taacgccccg gcaattaagg tctataaaaa atacggcttt gaaattgaag ggactggtaa
    17461 gaagtacgca ttgcgtaatg gtgaatatgt cgatgcatat tatatggcgc gggtgaagta
    17521 agatagtgcc ctttttctga gatggaaaaa gggtgtcatt caaaatcgac ataccttcct
    17581 ttaaggtatt tatttaccca atacatatat tgagatttaa ctcataatta cttcctaaag
    17641 tgtaatattt tattttttaa tatatacgcc tacaatttcc tggagtaaat aaataacaat
    17701 taacaagcat aatattgcca ttgataaaat agcatgccat aaaaggactt ttcagggatg
    17761 agtaatattg tttacctgac agtaacggga gaacaacaag gaagcatctc cgcaggttgt
    17821 gggacttctg agtctacagg taatcgttgg cagagcgggc atgaggatga aatatttaca
    17881 ttctcactct taaataatat taataatacg gggcttggtt cacagttcca tggtataaca
    17941 ttttgtaaat taattgataa aagcactcca ttatttatta attccattaa caataatgaa
    18001 caattattta tgggatttga cttctatcga ataaatagat ttggtagatt ggaaaagtat
    18061 tattatatac aactaagagg cgctttttta tcggctattc atcaccagat cattgaaaac
    18121 caactggata cagaaacaat aactattagt tatgaattta tcctctgtca acatcttatc
    18181 gcaaataccg agttcagcta tttggcactc cctgaaaatt ataaccgatt gtttttacca
    18241 aattcaaaaa accaaacaaa taatcgtttc aaaacgttaa acagcaaagc tattggcagg
    18301 ctacttgctg ctggtggcgt atacaatggg aacattgaag gattcagaga tactgcggaa
    18361 aaactgggtg gagatgcaat aaaaggctat gatcaaatac taaatgaaaa aacagcgggc
    18421 atagcgatag caacagcatc tattctttta acaaagcgtt ctaatgttga tacatataca
    18481 gaaataaata gttacttagg caaacttaga ggtcaacaaa aacttcttga tggtatagac
    18541 ataatagaaa taatatacat taagagacct tcaaaagact tagctaactt acgaaaggag
    18601 tttaataaaa ctgtaagaaa aaattttctt atcaaacttg caaaaacctc cgaagcatct
    18661 ggaagattca acgccgaaga ccttttaaga atgagaaagg gcaatgttcc tctaaattat
    18721 aatgttcacc ataaactatc tctagatgat ggtggtacta atgatttcga aaatttagta
    18781 ttaatcgaaa acgaaccata tcataaagtt tttactaaca tgcaatcacg aatagctaag
    18841 ggaatattag taggtgaaag caaaatcact ccctgggcca ttccatctgg ctcaatttat
    18901 cctcccatga aaaatgtaat gaacgcatcc cacacaatta aacacggagc agtatgatgg
    18961 ttaaaagggt gaaactggca ggtgtggagc ctcgcagatt tccggcatca tagtgcccaa
    19021 tacgggatgc ataaagctcc ctcagactag aaactccaca aatgaaatat acaccggttg
    19081 gcgttgatat cgcaaaacat gtcattcaga ttcacttcat caatgagcac acag
//
LOCUS       DADLFA010000071        18706 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000071 DADLFA010000000
VERSION     DADLFA010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18706
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.071"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(151..699)
                     /gene="pgsA"
                     /locus_tag="JQH65_002074"
     CDS             complement(151..699)
                     /gene="pgsA"
                     /locus_tag="JQH65_002074"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAY6139485.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLLFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(756..2588)
                     /gene="uvrC"
                     /locus_tag="JQH65_002075"
     CDS             complement(756..2588)
                     /gene="uvrC"
                     /locus_tag="JQH65_002075"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAY6139486.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEDEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(2585..3241)
                     /gene="uvrY"
                     /locus_tag="JQH65_002076"
     CDS             complement(2585..3241)
                     /gene="uvrY"
                     /locus_tag="JQH65_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HAY6139487.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            3536..3712
                     /locus_tag="JQH65_002077"
     CDS             3536..3712
                     /locus_tag="JQH65_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6139488.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            3699..3923
                     /locus_tag="JQH65_002078"
     CDS             3699..3923
                     /locus_tag="JQH65_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HAY6139489.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(3990..4738)
                     /gene="sdiA"
                     /locus_tag="JQH65_002079"
                     /pseudo
     CDS             complement(3990..4738)
                     /gene="sdiA"
                     /locus_tag="JQH65_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(4968..5720)
                     /gene="yecC"
                     /locus_tag="JQH65_002080"
     CDS             complement(4968..5720)
                     /gene="yecC"
                     /locus_tag="JQH65_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HAY6139490.1"
                     /translation="MSAIEVKNLVKKFQGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     LGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(5717..6385)
                     /gene="tcyL"
                     /locus_tag="JQH65_002081"
     CDS             complement(5717..6385)
                     /gene="tcyL"
                     /locus_tag="JQH65_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HAY6139491.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPILSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(6400..7386)
                     /gene="dcyD"
                     /locus_tag="JQH65_002082"
     CDS             complement(6400..7386)
                     /gene="dcyD"
                     /locus_tag="JQH65_002082"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HAY6139492.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLNLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(7491..8291)
                     /gene="tcyJ"
                     /locus_tag="JQH65_002083"
     CDS             complement(7491..8291)
                     /gene="tcyJ"
                     /locus_tag="JQH65_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY6139493.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(8379..8930)
                     /gene="fliZ"
                     /locus_tag="JQH65_002084"
     CDS             complement(8379..8930)
                     /gene="fliZ"
                     /locus_tag="JQH65_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HAY6139494.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQAHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDENGWQVEQQSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(8976..9695)
                     /locus_tag="JQH65_002085"
     CDS             complement(8976..9695)
                     /locus_tag="JQH65_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HAY6139495.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLSTKLGKL"
     gene            complement(10015..11721)
                     /locus_tag="JQH65_002086"
     CDS             complement(10015..11721)
                     /locus_tag="JQH65_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HAY6139496.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRILELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGKGTIANKAATVSDLTAAGATGTGPYAVTTNNTALSASDALS
                     RLKTGDTVTTTGSSAAIYTYDAAKGNFTTQATVADGDVVNFANTLKPAAGTTASGVYT
                     RSTGDVKFDVDANGDVTIGGKAAYLDATGNLSTNNAGIASSAKLSDLFASGSTLATTG
                     SIQLSGTTYNFGAAATSGVTYTKTVSADTVLSTVQSAATANTAVTGATIKYNTGIQSA
                     TASFGGANTNGAGNSNDTYTDADKELTTTASYTINYNVDKDTGTVTVASNGAGATGKF
                     AATVGAQAYVNSTGKLTTETTSAGTATKDPLAALDEAISSIDKFRSSLGAIQNRLDSA
                     VTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLL
                     QG"
     gene            11987..13393
                     /gene="fliD"
                     /locus_tag="JQH65_002087"
     CDS             11987..13393
                     /gene="fliD"
                     /locus_tag="JQH65_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HAY6139497.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAKNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKKQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            13418..13828
                     /gene="fliS"
                     /locus_tag="JQH65_002088"
     CDS             13418..13828
                     /gene="fliS"
                     /locus_tag="JQH65_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HAY6139498.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            13828..14194
                     /gene="fliT"
                     /locus_tag="JQH65_002089"
                     /pseudo
     CDS             13828..14194
                     /gene="fliT"
                     /locus_tag="JQH65_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015018.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
     gene            14272..15759
                     /gene="amyA"
                     /locus_tag="JQH65_002090"
     CDS             14272..15759
                     /gene="amyA"
                     /locus_tag="JQH65_002090"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HAY6139499.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYNLFDLGEFDQKGSIPTKYGDKVQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVITDEHGEATFFCNGGSVSVWVIEEVI"
     gene            complement(15793..16206)
                     /locus_tag="JQH65_002091"
     CDS             complement(15793..16206)
                     /locus_tag="JQH65_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY6139500.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLALMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            16393..17598
                     /gene="yedE"
                     /locus_tag="JQH65_002092"
     CDS             16393..17598
                     /gene="yedE"
                     /locus_tag="JQH65_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HAY6139501.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            17595..17828
                     /gene="yedF"
                     /locus_tag="JQH65_002093"
     CDS             17595..17828
                     /gene="yedF"
                     /locus_tag="JQH65_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HAY6139502.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVRDIQQDGPTIRYLIQK"
     gene            17935..18606
                     /locus_tag="JQH65_002094"
     CDS             17935..18606
                     /locus_tag="JQH65_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAY6139503.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPFFIYRADGQPIFMAAIGSTPFEHGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAIGNVKNQGAEL
                     IQPVC"
BASE COUNT         4634 a         4831 c         4573 g         4668 t
ORIGIN      
        1 ttcattaagc aatcagttaa tcacttgatt ttattatcgt ctggcaatca gtgccgccgt
       61 tcgatgcgtt gcattctact tacctggcgc gatgagtcaa cgatattttt caccactttt
      121 gatcgtttgc tgaaaattac gccgaaacga tcactgatca agcaaatctg cacgcgcagc
      181 gctcaaatat tgcaacattg accacagagt cagcaccgca gccacaaaga aaagtgcaat
      241 accggcgtac tcaacccaaa tgttcggacg ccacagcagc caggccaacg ccaccatctg
      301 ggcagtggtt ttcactttcc caatccagga gacagccacg ctactgcgtt tacccaactc
      361 cgccatccat tcgcgtagcg cagaaataat aatttcacgg gcgatcatcg ttgccgccgg
      421 tagggtcacc caccagctgt gataatgctc ggttaccagc accatggcga tagccacgag
      481 aactttatct gctacagggt caaggaaagc accaaaccgg gtactctggt tccagcggcg
      541 tgccagaaaa ccatcgaacc agtcagtcac cgccgcgacg cagaaaatga gcgcggcggc
      601 aaacggcgac caggtgacag gcagataaaa gagcaataca aagaatggga taaggatgac
      661 acggaacagt gtaagcaacg tagggatatt aaattgcata atgacgggta actatctgtt
      721 gtcagtaaga ttacccctat gttgctacag agacatcaat gtttcaacga ccagaagatc
      781 ttttctgcca gaccttgcga aatacccggc acttttgcaa tttcctcgac gctggcgtta
      841 cgtaaacctt gcaaaccgcc catatatttc aacaacattt gccgacgttt tggcccgacg
      901 ccttcaatgg tttccaggga actggtattt ttgaccttcg cccgtttttt acggtgcccg
      961 ccaatcgcgt gatcatgtga ttcatcgcga atatgctgga taacatgcag cgcgggtgaa
     1021 tctggcggca aactaaatcc ctcatcttcc ggctcaaaga acagcgtttc cagtccagcc
     1081 ttacgatctg ctcctttggc aacgccaagt agcagcggat gatttttatc ccatgagaca
     1141 tccagttcgg cgaagacatt tttcgcctgc gcaagctggc ctttgccgcc gtcgataagg
     1201 atcacatccg ggatcttact gtcgtcaatg gctttaccat aacgccgacg cagcacctga
     1261 ttcatcgccg cataatcatc gcccggcgtg atgcctgtaa tgttatagcg ccgatactcc
     1321 gcacgcagcg ggccgttagc atcaaacacc acacaggaag cgacggtttg ttcgcccatg
     1381 gtatggctga tgtcaaagca ctccatccgc ttcacttccg gcaatttcaa cacgctggca
     1441 agcgcggtca gtcgctggtg aacggtagat tgctgcgaaa gtttgctggt taaggccgtc
     1501 gccgcattgg tgcgcgcgag tttcagataa cgcgccctat cgccgcgagg tttggtttga
     1561 acattaatct tgcgtcccgc cagttctgaa agggaatcgg cgagcagcgt tttatcgcta
     1621 agattaaaat cgagcaggat ctcacccggt aaggtgcgca tctggctgcc ttgtaaatag
     1681 aactgaccta cgaaggtttc taccacctcg ctcagttccg taccgccagg cactttcggg
     1741 aaatagctgc ggctgccgag cactttgccc tgacgaatga acaatacgtg gacacaagcc
     1801 atgcccgcat cgaacgccac accaataacg tcaaggtcgt cgccggtatt ggaaacaaac
     1861 tgtttttcgg tgacgcgtcg caccgcctga atttggtcgc gaatacgtgc cgcttcttca
     1921 aactccagat tctgactggc agtttccata cggctaatga gttgcgtaag cacctgatca
     1981 tctttgccag acaaaaacag gcgcacatac tcgacctgct gagcgtattc ttcttcactc
     2041 accagtcctt caacgcacgg tcccagacag cgccctatct gatattgcag acacggacgc
     2101 gagcgattgc gataaacact attttcgcac tggcgaatgg ggaaaatctt ttgcagtagc
     2161 gccagagttt cacgtacggc atagccattc gggaacgggc cgaaatattc acctttggca
     2221 tgcttcgcac cacgatgcat cgccagacgc gggtgggtat cgccactcag gaagataaaa
     2281 ggataagatt tatcatcgcg tagcaaaacg ttgtaacgcg gctgatagag tttgatgtag
     2341 ttgtgttcca gcaacagcgc ttcggtttct gtatgagtaa ccgttacatc aatttgctga
     2401 atctgggcga ccagcgcttc ggttttgcgc gaagcgaggt tgctacggaa atagctggaa
     2461 agccgttttt tcaggtcttt cgctttgccg acatagataa ccgtaccacc agcatcgtac
     2521 atgcgataaa cgcctggctg gctggttacg gtttttaaaa acgcttttgc gtcaaactga
     2581 tcactcactg acttgataat gtctccgcat tacacagacc atggcgaatt gccaggtgag
     2641 tcagctcaac atcgccatga atgtttagtt tactgaacat acgatagcgg tagctgttca
     2701 ccgttttcgg actgagattg agctgttctg agatctcatt gaccttctgg cccttggtga
     2761 tcatcagcat aatctgcaat tcacgttcag acaaactggc aaatgggctt tctgtttttt
     2821 ctggttcgat ctggcttaac gccatttgtt gagcgatgtc agaagcaatg taacgctgcc
     2881 ctgaatagac agaacgaatc gcactcacga cttcctgcgg agccgcgcct ttgctgaggt
     2941 agcccgcagc accggcctgc atgactttcg ctggtaaagg gttttctgta tgaacggtaa
     3001 gcatgatgat tttgacatca gctgtggaac gcgcgatttt acgcgtcgcc tcaagaccgc
     3061 caatgcccgg catactcatg tccattagca ccacgtcaac ggcatttgtc cggcaccact
     3121 taacggcgtc ttcaccgcac gatgcctcac cgacgacttt tatacccttt atatcttcca
     3181 gaatgcgtcg tatccctgcg cgcaccagtt cgtggtcatc aacaagtaga acgttgatca
     3241 aagaaatatc tccagaaata gggataacgc tactgatagt tagtcattcg tatattagcg
     3301 gtttttattg caactttgaa acgttaaaaa tggtgggtat tcgatttttc tctcgttttt
     3361 gtcaattcga ttgtccacag ttatggtcac gcccgtcacc aacggcttac cggatcgttt
     3421 cacagtaagc cctacgatga aatgtgacaa aaattgactt tattcagcaa aaatgaaaat
     3481 cagcccgctg aatatgtaac attaattaac ccgaacaata cgggcaaaaa cattcatgat
     3541 aaaaatattt attggtcatt atattaacgt attttatagc actgccgata tcacgctcaa
     3601 aaaacaacca ctgctatttt tagcaaagct tatggtatac tccgccgcct taacattttt
     3661 caccgcaaat tttcattgca acatgacgag gaaaataaat gagtacgcct gatttttcta
     3721 ctgccgagaa taatcaagaa ctggcaaatg aagtctcctg cctgaaagcg atgctgacgc
     3781 tgatgctgca ggcgatggga caagctgacg cgggccgcgt gatgttaaag atggaaaaac
     3841 agcttgcgct gatcgaagac gaaacccagg ctgcagtatt ttccaaaacg gttaagcaaa
     3901 ttaaacaagc ctaccgtcag taatataaaa ccggctgaga gcgtgccttt cagccggttt
     3961 ttgcatctgg cacgcagaaa gaaaagcgat caaattaagc cagtagcggc cgcgtaacag
     4021 gccacctggg tcttatttgg tgcattgatt tttttctgca tgtttttctg atggaaattg
     4081 accgtattct cagagattga caaaatcatc gctatctctg ctgatgtctt cccttccgcc
     4141 gtccatttca gaatttcttt ttcgcgcttg ctgaaattca tctctggcgt catcactatt
     4201 tcatcattta aacgcatcag agccatcaga ctttcgcgca ccagtaactg catttttaat
     4261 tgcagttcat cactaagaat gggtatttcg cgcgtgctgc aacgggaaaa ggacaaaaag
     4321 cccagcgccc gatttggcag cattaaatac tgagtgacac cgcggcgtaa accatgtgcg
     4381 tgcgcggctt cccataatgg ctgtgcttcg ctgaataagt catcattcca cattaaatgg
     4441 ccctgactaa agttttcagg gttcagcaca ggatcaattg cgagaaaatt agcacaggat
     4501 caattgcgag aaaatttttt gcctgataat aactaaccca cgactcaggg taattggtgt
     4561 agaaagccac tttaggtcgc gtgaatggta ccgggtggcg aacacataac gaatagtaat
     4621 cgtactccag ctgctgagcc tgaagctcta tttcatggta gacctcttct gcggcctcca
     4681 tcctctgaaa acgcaacagc atcgtgcgac gccaactgaa aaaatccgta tcctgcatag
     4741 taaaccgcaa cgcccctgag agtgagtatc atttataatg ataattcaaa ctatcacata
     4801 aataagattt atatataatt tatattattc aggcaatgaa ttacttttgc aagccatcgc
     4861 attctcttat gttattaatg agttatgctg atttgttaag cagttttatc acgcttgaaa
     4921 tggcgtcccg ccccgacagg tgaatcgtcg gggctgattt tttcttatta ttgcagcaga
     4981 aacttctcga ggaactgtcg ggtgcgaggc tgctggggat cggcaaataa cgcttttgct
     5041 gccccctgct cgactatccg ccccaggtcc ataaaaatcg cccggtccgc aacatcccgg
     5101 gcaaagctca tttcgtgcgt cacaatcacc atcgtgcgct tttcctgcgc cagctggcgg
     5161 attgtgttca ggacttcgcc caccagctct ggatccagcg ctgacgttgg ctcgtcaaac
     5221 aaaatcacct caggacgcat tgccagcgca cgcgcaatcg ctacacgctg ctgttgaccg
     5281 ccagacaaac gacgtggata gctggtttct ttaccggcca gcccaacttt cgccagcagc
     5341 tcgcgagcgc gcgccgtggc ctcttctttc ggttcgcctt tgacgatcac cggcccttca
     5401 ataatgtttt ccagcaccgt acgatgcgga aacaaattaa agttctggaa gacaaacccg
     5461 acgtgctgac gcaactggcg aatcagcgat ttttgctgac ttaatgaacg tgcagtatca
     5521 atagtgatat cgccaacggt gatcgttccc gcttccggtt gttccagcag atttatgctg
     5581 cgtagcaacg tggttttgcc ggaaccactc ggaccgataa ttgccaccac ttcgccaggc
     5641 tttacctcga ggtcgatacc gtgcagcacc gtctgacctt ggaatttttt caccaggttc
     5701 ttaacttcaa tggcactcat ttgggttctc tctcctggcg attaagttga ttctcaaaat
     5761 ggttctgcaa cgtcgataac acagtcgcca tgatccagta gatgagcgaa gccgccaggt
     5821 acatggtgaa cacctccagc gtgcgcgagg taatcaactg cgcctgacgg aacagctccg
     5881 gcacctggat tgtcgcggcc agcgacgtat cttttaccag actgataaag ctgttcgaca
     5941 gcggcggcaa cgccacccgc gccgcctgtg gcaaaatcgc gcgacgcatg gtctgccacg
     6001 gcgtcatacc aatactggca gcggcttccc actgaccttt atcaatagaa gaaatggcgg
     6061 cgcgcagcgt ttcggcggca taggcggcag tgttcagcga cagaccaatc attgctgaca
     6121 gaatgggatc taattcgata ccaaactgcg gcaggccgta atagatcata aacagttgtg
     6181 cgatgagcgg cgtaccacga aaaatagaga tataaaaacg cgccagccag cgcacgggcc
     6241 agattggcga caggcgcatc agcgcgagaa taaacccgag tagtaagcca aaaaacatgc
     6301 cgccgatact gagttgcagg gtatacccag cccctttcaa caggaacggc aaggaatcaa
     6361 taaccagttg tatactttct tgcatgagcg ttcccggagc taaacgtggg gatgataggc
     6421 gaacagcgca ggcgcgccgc cggtatgaat aaacagaatc ggcccttcat ctttgaagcg
     6481 tttctgactg ataccgtcaa tcagccccgc catcgctttt ccggtataca caggatcaag
     6541 cagaatgcct tccagacgcg ccagcaattt cactgcttcc atgccttcgt cgttcggcac
     6601 gccgtagcca ggtgcaaaat agtcatccca gagtaaaatt tccgctgatg cggtcagctc
     6661 cagttctttc gcaatcgcct gttgtaggtt aaccactttc ggcaattgat cggcaacgga
     6721 acgcgacacg gtcacgccaa tcagttcgct ttcaggcatc aggtgttcca gcccaacagc
     6781 caatccggcg tgagttccgg cactgcccga tgcgaccacc accgacgaaa tattaaccgc
     6841 cccttcacac tgttgcgcga tttccagcgc actctccaca taacccagcg cgcccagagc
     6901 attagaaccg ccaaccggaa tgacatacgg gcgaaagcct tgtgcttcga ctcgcgtcgc
     6961 cagctcttcc agttgggcat tgggatcggt cagtgcgtcg cacatttcaa tctgggtatt
     7021 gaacagattc agcaacaaac gattgccgtt ggttaaatag ttttctgcgg ttgtgccaat
     7081 aggattttcc agcagcgcca cgcagtgcag gccgagtttc gccgcaactg cggcagtctg
     7141 ccgcacatgg ttagactgga tcgcgccggc agtaatcagc gtatcggcac cttcgcgcag
     7201 ggcatctgcc gcgagaaatt ccagcttacg taatttattg ccgcccattg ccatgggtgt
     7261 gacgtcatcc cgtttgatga aaatttcccg tccaagataa tcagaaaagc gcggcagata
     7321 ttcgagcggc gttggcgcgc cgataaactc cagccgtgga aaacgggtta aattatgcag
     7381 tggcataata gcctccgatg tgtgttgttg tgattttctt attatgcacg ctgaaaacgc
     7441 gtaaataaaa aaggcgctag tgaaagcgcc cttttttgtc attatgctga ttatttggtc
     7501 acatcagcac caaaccattt ttcggaaagg gcttgcagag tgccatcttt ttgcatttcc
     7561 gcaattgcat cgttcactgc tttcagcagg tcttcatttc ctttacgcag cgccacgcca
     7621 gactcctgac gggagaatgc ttcaccggtt actgccagcg tatcgttggt tttcttcacc
     7681 agatccagcg ccgccagacg gtcaacgagg atcgcatcga tacggcctac gcgcagatcc
     7741 tgatatttgg tcgggtcatc atcataggta cgcacatcga cgccctgaac attctgccgc
     7801 agccactctt catagttggt gcccagaccg acacccactt ttttgccttt cagatcagca
     7861 gccgttttaa tggtgccttc gttacctttt ttcaccagcg cctgaatacc agaaatggtg
     7921 tacggggtag agaaatcgta ttttttcttg cgctcatcag aaatggtgac ctgattaatc
     7981 accacatcaa tacgtttaga gtccagcgac gctagcatac cgtcccattt ggtcggtttt
     8041 agtgacgcct caacgccaag atgttttgcc agctgttggg caaattccac ttcaaaaccg
     8101 gttaatttgc cgtcatcccc ctgaaaactg aacggcggat aagttccttc cagccctacc
     8161 agtagcgtac cgcgctcttt gactttatta agcagacctt catctgcaaa acttttaacg
     8221 ctcatgcccg caaccagcgc cacggccatc acacccatca atgcctgacg tcccagatgt
     8281 gctaatttca tattcacccc gaatgttgtt atgtctgttt gcagtgtaga gccattgttt
     8341 gtaaacacaa aaacaactcc gctacatctt attcttattt aatatatatc agaagcaggc
     8401 aggctggagg atttctgcac aagtccggta cacgtttgac gctgatagtg ttgatacttc
     8461 cgtaatgcga tgcggtaatt gttggtgctg gtggtgggca gccacggggc gaggatttca
     8521 tcaaggaaac cgtcctgcag ctgatcgagg gaaatatttt gctcatgtag atgattcccc
     8581 aaacggcgca aacgaacgac atattcacgc accgtacctg ggctcatttc agtttgctca
     8641 aaaagaaatt gcttaaagcc gataatatcg aaaaaatcac tctgcgtttt gcaatgtaaa
     8701 tcaccgcaaa atcggcacaa tgccgcccat gactgttgtt ccacttgcca gccattttca
     8761 tcaagcagcg tgtccaggcg ggaaatctcg attttattca ctattttgcc gtcgcgaact
     8821 aaggtaatgc gatcgagtaa tttacggcaa tgcgcgcaat gggcctggct gtgtttaaag
     8881 tctttaagat agcggcttaa tggccgtctt ttcaggtgct gcaccatcat taagaactcc
     8941 tggtagtcaa agttaaagtg cggcatttac tgacgttata acttacccag tttagtgctt
     9001 aaccgtttaa tggcctggct gtgtaactga ctgacccgcg attccccgac ctccagcacc
     9061 gcgccaatct ctttgagatt cagctcttcc tggtaataga gggttaatac cagtttttcg
     9121 cgctccggca acgtttcgat agcttccatc acccgctggc gcagattact gtccagcagt
     9181 tgttgtagcg ggttttctcg ctgatgatca tcagtaacca gttcgatgct atcgccgtgc
     9241 tcttcgcgcc actcatcgta ggagaaaagc tggctgttat tggtgtcgag caacatttgg
     9301 cgataatcgg caatatcgat ccctaaacgt tccgctacct cagtttccgt ggcgttgcgg
     9361 ccaagttcct gctccagttg ccctattgcc tgtgccactt cacgcgcgtt gcgtcgcacg
     9421 ctgcgcggca cccagtcacg gctgcgaagt tcatccagca tagcgccacg gatacgctgc
     9481 actgcgtaag ttgtaaatgc cgttccttgt agggcgtcat agcgttcgac ggcattaagt
     9541 aacccaatgc cgcccgcctg tagcagatcg tcaagttcca cgctcgcggg cagtcgaacc
     9601 tgcaggcgca atgcttcgtg acgcaccagc gggacataac gctgccacag cgagtgttta
     9661 tccattacac cttcagcggt atagagtgaa ttcacgataa acagccctgc gttatatgag
     9721 ttatcggcat gattatccgt ttctgcaggg tttttaatcg gacgattagt gggtgaaatg
     9781 aggggttatt tgggggttac cggtaaattc catgcagaaa aaaccccgcc ggtggcgggg
     9841 aagcacgttg ctgacaaatt gcgctttatg ttgccggatg cggcgtaaac gccttatccg
     9901 gcctacaaaa atgtgcaaat tcaataaatt gcaattcacc ttgtaggcct gataagtgca
     9961 gcgcatcagg caatttggcg ttgccgtcag tctcagttaa tcaggttacg acgattaacc
    10021 ctgcagcaga gacagaacct gctgcggtac ctggttggct tttgccagca cggagttacc
    10081 ggcctgctgg atgatctgcg ctttcgacat gttggacact tcggtcgcat agtcggcgtc
    10141 ctgaatacgg gactgcgctt cagacaggtt ggtagtggtg ttgttcaggt tggtgactgc
    10201 agaatccaga cggttctgaa tagcacccag ggatgaacgg aatttgtcga tggagctgat
    10261 agcttcatcc agggcagcca gaggatcttt ggttgcagtg cctgcactgg tggtttcagt
    10321 ggtcagtttg cctgtagagt taacataagc ctgtgcccca acagtagctg caaatttacc
    10381 agttgcacct gcgccatttg aagctacagt tactgtaccg gtatccttat cgacgttgta
    10441 gttgatagtg taagatgcgg ttgtggtgag ctctttgtct gcatcagtat aggtgtcatt
    10501 cgaattacca gcaccattag tattcgcacc accgaaggac gccgttgcag actgaatacc
    10561 tgtattatac ttaattgtcg caccagtaac tgctgtgtta gccgttgcag cactctgcac
    10621 tgtgctcagt acagtatcag cgcttacagt tttggtgtag gttacgccag aagttgccgc
    10681 tgcaccaaag ttataagttg tgccagacaa ctggatagaa ccagttgtcg ctaaggtact
    10741 accgctagca aacagatcgg acaatttcgc tgaagatgca atgccggcgt tgtttgtaga
    10801 taggttacca gtggcgtcca ggtacgcggc tttaccaccg atggtcacat cgccattagc
    10861 atctacatca aacttcacat caccagtact acgagtataa acacctgatg cagtagtgcc
    10921 agccgctggt ttcagagtat tcgcaaagtt aacaacatcg ccatctgcaa ctgttgcttg
    10981 agtggtgaag ttccctttag ccgcatcata agtatagatc gcagcactcg agccagtagt
    11041 agtaactgta tctccggttt tcaggcgaga cagtgcatcg ctagcgctga gtgctgtatt
    11101 gtttgtggtc acagcataag gacctgttcc cgttgcacca gcagcggtca gatcgctgac
    11161 tgtagcagct ttgttcgcaa tagtgccttt gccattcacg ttaaaaccat tcagccccag
    11221 cgtatctgag tcaattttct tcagatcaat cgtgatagtc tggccgtcat tcgcaccaac
    11281 ctgaattttc attgaaccgt ctttcgccag tacgttcacg ccgttgaact gggtctggcc
    11341 agatacgcgg tcaatttcgt ccagacggga tttgatttcg tcctgaatgg agtccagatc
    11401 cgaatcggag ttagtcccgg tagaagcctg aaccgtcagt tcaagaatac gctgtaagtt
    11461 gttgttgatt tcggacagcg cgccttcagt ggtctgcgca acggagatac cgtcgttggc
    11521 gttacgggcc gcctgagtca ggcctttaat attagaagta aaacggttag caatcgcctg
    11581 acctgcggcg tcatccttcg cgctgttaat acgcaagcca gaagacagac gctcgataga
    11641 actcgacagc gcagactggt tcttgttgat attattttga gtgatcagcg agaggctgtt
    11701 ggtattaatg acttgtgcca tgattcgtta tcctatattg caagtcgttg attacgtatt
    11761 gggtttccac ccgtcggctc aatcgccgtc aaccctgtta tcgtctgtcg taaaacaacc
    11821 tttagaattt ttttcacaaa cagccatttt ttgttagtcg ccgaaatact cttttctctg
    11881 ccccttattc ccgctattaa aaaaaacaat taaacgtaaa ctttgcgcaa ttcaggccaa
    11941 taaccccggt attcgtttta cgtgtcgaaa gataaaagga aatcgcatgg caagtatttc
    12001 atcgctggga gtcgggtcag gtctggattt aagttcaatc cttgatagcc tcaccgccgc
    12061 gcaaaaagcg acgctaaccc ctatttcaaa tcagcaatcg tcgtttaccg ctaaacttag
    12121 cgcctacggt acgctgaaaa gcgcgctgac gactttccag accgccaata ctgcattgtc
    12181 taaagccgat cttttttccg ccaccagcac taccagcagc accaccgcgt tcagtgccac
    12241 cactgcgggt aacgccatcg ccgggaaata caccatcagc gtcacccatc tggcgcaggc
    12301 gcaaaccctg accacgcgca ccaccagaga tgatacgaaa acggcgatcg ccaccagcga
    12361 cagcaaactc accattcaac aaggcggcga caaagatccg attaccattg atatcagcgc
    12421 ggctaactca tcgttaagcg ggatccgtga tgctatcaac aacgcaaaag cgggcgtaag
    12481 tgcgagcatc attaacgtgg gtaacggtga atatcgtctg tcagtcacat caaatgacac
    12541 cggccttgat aatgcgatga cactctcggt cagcggtgat gatgcgctac aaagttttat
    12601 gggctatgac gccagtgcca gcagcaacgg tatggaggtc tcggtcgccg ccaagaatgc
    12661 gcagctgacg gtcaacaacg tcgccattga gaacagcagc aacaccatca gcgacgcgct
    12721 ggaaaacatc accctgaacc tgaacgatgt caccacgggc aaccagacgc taaccatcac
    12781 tcaggacacc tccaaagcgc aaacggcgat taaagactgg gtgaatgcct ataactcgtt
    12841 aatagatacc ttcagtagcc tgaccaaata caccgccgta gatgcgggag ctgatagcca
    12901 gagttctagc aatggcgcac tgctcggcga ctccacgctg cggacgattc agacgcagtt
    12961 gaagtcgatg ctgagtaata ccgtcagttc ttccagctat aaaacgctgg cgcagattgg
    13021 tatcacgacc gatcccagcg atggcaaact ggaactggat gccgacaaac tcaccgctgc
    13081 actgaaaaaa gatgccagcg gcgtaggtgc attgattgtt ggcgatggta aaaaaaccgg
    13141 catcacgacc accatcggca gcaacctgac cagttggctt tcgacaacgg gcattattaa
    13201 agccgctacc gatggcgtta gtaagaccct gaataagtta actaaagact acaacgccgc
    13261 cagcgatcgc attgatgcgc aggtcgcgcg ctacaaaaaa caatttaccc aactggacgt
    13321 tttaatgacc tcgttaaaca gcaccagcag ctacttaacg cagcagttcg aaaacaacag
    13381 taattccaag taatcattat tcatcgggag acaggtcatg tacgcggcaa aaggcaccca
    13441 ggcctatgca caaattggcg tcgaaagcgc cgtaatgagc gccagccagc agcagctggt
    13501 caccatgcta tttgatggag tgctgagcgc actggttaga gcgcgcctgt ttatgcagga
    13561 caacaatcag caaggcaaag gcgtctcttt gtcaaaagcg atcaacatca ttgagaacgg
    13621 actgcgggtg agtcttgatg aagagagcaa agacgaacta acccaaaact tgattgctct
    13681 ttatagctat atggtcaggc gcttgctgca agccaattta cgcaacgatg tctccgcagt
    13741 cgaagaagtg gaagcattaa tgcgcaatat tgccgatgcc tggaaagagt cgttactctc
    13801 cccttctttg attcaggacc cagtctgatg aaccatgcac cgcatttgta tttcgcctgg
    13861 caacaactcg tcgaaaaaag ccagctcatg ttacgcctgg caacggaaga acaatgggac
    13921 gaactcatcg ccagcgaaat ggcgtatgtg aatgcggtgc aggagtatag cacacctgac
    13981 tgaagaggtt gccccgtcca ccacgatgca ggagcagctc cgcccgatgc tgcgcctgat
    14041 tctcgacaac gaaagcaagg taaagcagtt attacagatt cgtatggatg aactggcgaa
    14101 actggtcggt cagtcatcgg tgcaaaaatc ggtgttaagt gcctatggcg atcagggcgg
    14161 ctttgtgctg gctccgcaag ataacttctc gtgaatctta acgagctaat ggttcgcgaa
    14221 taatccgatt acggctacgc ttctaatgtt ccccttgaat ggagtcgaag aatgcgtaat
    14281 cccacgctgt tacaatgttt tcactggtat tacccggaag gcggtaagct ctggcctgaa
    14341 ctggccgagc gcgccgacgg ttttaatgat attggtatca atatggtctg gttgccgccc
    14401 gcctataaag gcgcatcggg cgggtattcg gtcggctacg actcctataa tttatttgat
    14461 ttaggcgagt ttgatcagaa aggcagcatc cctactaaat atggcgataa agtacaattg
    14521 ctggccgcca ttgacgccct gaaacgtaat gacattgcgg tgctgttgga cgtggtagtc
    14581 aaccacaaaa tgggcgcgga tgaaaaagaa gctattcgcg tgcagcgtgt aaatgctgac
    14641 gaccgtacgc aaattgatga agaaatcatt gagtgcgaag gctggacgcg ttacaccttc
    14701 cccgcccgtg ccgggcaata ctcacagttt atctgggatt tcaaatgttt tagcggtatc
    14761 gaccatatcg aaaaccctga tgaagatggc atatttaaaa ttgttaacga ctacaccggc
    14821 gaaggctgga acgatcaggt tgatgatgaa ttaggtaatt tcgattatct gatgggcgag
    14881 aatatcgatt ttcgcaatca tgccgtgacg gaagagatta aatactgggc gcgctgggtg
    14941 atggaacaaa cgcaatgcga cggttttcgt cttgatgcgg tcaaacatat tccggcgtgg
    15001 ttttataaag agtggatcga acacgtacag gaagttgcgc caaagccgct gtttattgtg
    15061 gcggagtact ggtcgcatga agtcgataaa ctgcaaacgt atattgatca ggtggaaggc
    15121 aaaaccatgc tgttcgatgc gccgctgcag atgaaattcc atgaagcatc gcgcatgggg
    15181 cgcgactacg acatgacgca gattttcacg ggtacattag tggaagccga tcctttccac
    15241 gccgtgacgc tcgttgccaa tcacgacacc caaccgttgc aagccctcga agcgccggtc
    15301 gaaccgtggt ttaaaccgct ggcgtatgcc ttaattttgt tgcgggaaaa tggcgttcct
    15361 tcggtattct atccggacct ctacggtgcg cattacgaag atgtcggtgg tgacgggcaa
    15421 acctatccga tagatatgcc aataatcgaa cagcttgatg agttaattct cgcccgtcag
    15481 cgtttcgccc acggtgtaca gacgttattt ttcgaccatc cgaactgcat tgcctttagc
    15541 cgcagtggca ccgacgaata tcccggctgc gtggtggtca tgtcgaacgg ggatgatggc
    15601 gaaaaaacca ttcatctggg agagaattac ggcaataaaa cctggcgtga ttttctgggg
    15661 aaccggcaag agagcgtaat aaccgacgaa cacggcgaag caaccttctt ttgcaacggc
    15721 ggcagcgtca gcgtgtgggt tatcgaagag gtgatttaaa ttcatccccg gcggcaagcc
    15781 ggggagattt cattacggca gtggcgtcgg taacggcgct ttatccagcg cggcggcaca
    15841 ctctgccgtc gggcgatcca cacgctccat cgtcatacca tcgtactcaa tggtgttacc
    15901 gtcacgctca acctcgtaca gctcacgttt tacggtaacg ttagtgagat catcagacat
    15961 caatgccaac ttacccggca cagcaatcac ccgctgccac tgacggcaat ccagagtatc
    16021 accctcttta gtgacaatca ggctgcctat cgcttccgga ctgaccagcg cacgttgcgg
    16081 ccctttggtc tgccagtagc ccgccagcca gtccggcgcg ggggttttca cgacattgtt
    16141 atagttttcg acttcggcac agcccgccag caacatgagc gcgcctgcaa ttgcgagttt
    16201 tttcatcatt cgtcctgcat gagaagaaaa gaggattgtg gcattaaagc cctgatgtcg
    16261 ccagcctttc caggttcaga gagaaatttg atctaactat tatttttgtg aattattacc
    16321 ccgaaaataa atttgcatga tgtatgattc gcgactcttg tttcaaacct tctgagttca
    16381 gaggctacat tcatgtcatg gcagcaattc aaacacgcct ggttgattaa attctgggca
    16441 cccatccctg cggtcatcgc ggcgggtatt ctctccactt actattttgg cattactggc
    16501 accttttggg ctgtcacggg tgaatttacc cgttggggcg gccagctcct gcagctgttt
    16561 ggcgtccatg ctgaagagtg gggttatttt aaaattatcc atctggaagg atcgccatta
    16621 acccgcatcg acgggatgat gatcctcggc atgtttggcg gctgctttgc cgcagcgctg
    16681 tgggccaaca atgtcaaact gcgcatgcca cgcagccgta tccgcattat gcaggccatt
    16741 attggcggta ttatcgctgg ttttggcgcg cgtctggcaa tgggctgtaa cctggcggcg
    16801 ttctttaccg gtattcctca gttctcgctg catgcctggt tctttgccat cgccactgcc
    16861 attggttcat ggtttggcgc acgctttacc ctgctgccca tcttccgtat tcccgtgaaa
    16921 atgcagaaag tttctgccgc ctcaccgctg acgcaaaaac cggatcaggc gcggcgtcgt
    16981 tttcgtctcg ggatgctggt ctttttcggt atgctgggct gggcgctgct cacggcgatg
    17041 aaccaaccaa aactggggct ggcaatgctg tttggcgtcg gctttggttt actgattgaa
    17101 cgtgcgcaaa tctgctttac ttcggcgttc cgcgatatgt ggatcaccgg acgtacccat
    17161 atggcgaaag caatcattat cggtatggcg gtgagtgcca tcgggatctt cagttacgta
    17221 cagttaggcg ttgaacccaa aatcatgtgg gcgggaccaa acgcggtaat tggtggttta
    17281 ctgtttggtt ttggcatcgt gctggcaggc ggctgcgaaa ccggctggat gtaccgcgcg
    17341 gtagaaggcc aggtgcacta ctggtgggtc ggtctgggca atgtgatcgg ctcgacgatt
    17401 ctggcgtatt actgggatga tttcgctccg gcgctggcca ccgactggga caaaatcaac
    17461 ctgctgaaaa cctttggccc gatgggtggc ctgctggtga catatttgct gttgtttact
    17521 gcgctgatgt tgattatcgg ctgggaaaaa cgcttcttcc gccgtgcggc accgcagact
    17581 gctaaggaga tcgcatgaaa aatatcgttc ctgattaccg ccttgatatg gtgggtgaac
    17641 cctgccctta tccggcagtc gcaacccttg aggcgatgcc gcagttaaaa aaaggggaaa
    17701 tcctggaagt ggtgagcgac tgtccacagt cgatcaataa tattccactg gatgcgcgta
    17761 atcacggcta tacggtgcgg gatattcagc aagacgggcc gaccattcgt tatttaattc
    17821 agaagtaatc cctcattccc gctgtggccc acagcgggaa catcgttgcc tctgacacat
    17881 agattttacc cgccccctct cctattcttt tcagtatgat ctcaggaggt ttctatgtgt
    17941 ggacgctttg cccaatccca aacgcgtgaa gattaccttg cgcttctcgc ggaagatatt
    18001 gaacgcgata ttccctacga tcccgaaccc attggcagat acaacgtcgc gccgggaacc
    18061 aaagttcttc tgctcagtga acgtgatgaa caccttcatc tggatccggt tttctgggga
    18121 tatgctcccg gatggtggga taaaccgccg ctgattaacg cccgcgtaga aactgccgca
    18181 accagtcgca tgtttaaacc gctctggcaa catggtcggg caatctgttt tgccgatggc
    18241 tggtttgagt ggaaaaaaga aggcgacaaa aaacagcctt tttttatcta tcgtgctgat
    18301 ggacaaccta tttttatggc cgcgataggt agcacaccat ttgagcatgg tgacgaagcc
    18361 gaaggatttt taattgtcac tgctgcagca gaccagggtc tggtagacat tcatgaccgc
    18421 cgcccgctgg ttctgtcgcc agaagctgca caggaatgga tgcggcaaga gattggcggt
    18481 aaagaagcat cagaaattgc taccaacggc tgtgtgccag caaaccagtt cacctggcac
    18541 ccggtatcgc gcgcgatagg taatgtcaaa aaccagggcg cggagttaat tcaacctgtt
    18601 tgttgacatc ctccacgccc tgaaggacga ggctccctgc tacgttcagg ctgtcgcctg
    18661 aatcatttcg gtgggttcct gcttcaacgg gcggcctgac tgcacc
//
LOCUS       DADLFA010000072        18505 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000072 DADLFA010000000
VERSION     DADLFA010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18505
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.072"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..893
                     /locus_tag="JQH65_002095"
     CDS             <1..893
                     /locus_tag="JQH65_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY6139504.1"
                     /translation="WFNIKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFIT
                     IGVLGIFLAIGWYMLYRNREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTM
                     WGMMLGFSGINYTAWLYLAWLPGYLQTAYNLDLKSTGLMTAIPFLFGAAGMLVNGYVT
                     DWLVKGGMAPIKSRKICIIAGMFCSAAFTLIVPQATTSMTAVLLIGMALFCIHFAGTS
                     CRGLIHVAVASRMTASVGSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTA
                     AGALAYIFLVRQPINDPRKD"
     gene            complement(942..2597)
                     /gene="tsr"
                     /locus_tag="JQH65_002096"
     CDS             complement(942..2597)
                     /gene="tsr"
                     /locus_tag="JQH65_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HAY6139505.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQRETSAVVKTVTPAAPRKMAVADSEENWETF"
     gene            complement(2716..3162)
                     /gene="hpaR"
                     /locus_tag="JQH65_002097"
     CDS             complement(2716..3162)
                     /gene="hpaR"
                     /locus_tag="JQH65_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HAY6139506.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
     gene            3434..4723
                     /locus_tag="JQH65_002098"
     CDS             3434..4723
                     /locus_tag="JQH65_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HAY6139507.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEDTAK"
     gene            4720..6186
                     /gene="hpaE"
                     /locus_tag="JQH65_002099"
     CDS             4720..6186
                     /gene="hpaE"
                     /locus_tag="JQH65_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HAY6139508.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRDHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            6188..7039
                     /gene="hpaD"
                     /locus_tag="JQH65_002100"
     CDS             6188..7039
                     /gene="hpaD"
                     /locus_tag="JQH65_002100"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HAY6139509.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            7049..7429
                     /locus_tag="JQH65_002101"
     CDS             7049..7429
                     /locus_tag="JQH65_002101"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HAY6139510.1"
                     /translation="MPHFIVECSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            7540..8343
                     /gene="hpaH"
                     /locus_tag="JQH65_002102"
     CDS             7540..8343
                     /gene="hpaH"
                     /locus_tag="JQH65_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HAY6139511.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            8354..9142
                     /gene="hpaI"
                     /locus_tag="JQH65_002103"
     CDS             8354..9142
                     /gene="hpaI"
                     /locus_tag="JQH65_002103"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HAY6139512.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLSDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIRESGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            9317..10693
                     /gene="hpaX"
                     /locus_tag="JQH65_002104"
     CDS             9317..10693
                     /gene="hpaX"
                     /locus_tag="JQH65_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HAY6139513.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGLLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            10703..11589
                     /gene="hpaA"
                     /locus_tag="JQH65_002105"
                     /pseudo
     CDS             10703..11589
                     /gene="hpaA"
                     /locus_tag="JQH65_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224927.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
     gene            11840..13402
                     /gene="hpaB"
                     /locus_tag="JQH65_002106"
     CDS             11840..13402
                     /gene="hpaB"
                     /locus_tag="JQH65_002106"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HAY6139514.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSANDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDADGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQHIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            13420..13932
                     /locus_tag="JQH65_002107"
     CDS             13420..13932
                     /locus_tag="JQH65_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HAY6139515.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            14325..16475
                     /gene="btsT"
                     /locus_tag="JQH65_002108"
     CDS             14325..16475
                     /gene="btsT"
                     /locus_tag="JQH65_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HAY6139516.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            16606..16809
                     /locus_tag="JQH65_002109"
     CDS             16606..16809
                     /locus_tag="JQH65_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HAY6139517.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            16820..17776
                     /gene="yjiA"
                     /locus_tag="JQH65_002110"
     CDS             16820..17776
                     /gene="yjiA"
                     /locus_tag="JQH65_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HAY6139518.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(17878..>18505)
                     /locus_tag="JQH65_002111"
     CDS             complement(17878..>18505)
                     /locus_tag="JQH65_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase"
                     /protein_id="HAY6139519.1"
                     /translation="PGSAILNVWLTSMLKRTVVAAVPMPFDKWYSASGYETTQDGPTG
                     SLNISVGAKILYEAVQGDKSPIPQAVDLFAGKPQQEVVLAALEDTWETLSKRYGNNVS
                     NWKTPAMALTFRANNFFDVPQAAAEETRHQAEYQNRGTENDMIVFSPTTSDRPVLAWD
                     VVAPGQSGFIAPDGTVDQHYEDQLKMYENFGRKSLWLTKQDVEAHKES"
BASE COUNT         4226 a         5124 c         4939 g         4216 t
ORIGIN      
        1 actggttcaa catcaaagag cgcggacgcc cgatgggctt cttcaacgca gcttctacca
       61 ttggcgttgc cgtaagccca ccgattctgg cggcgatgat gctggtgatg ggctggcgcg
      121 ggatgtttat taccattggt gtactgggga tttttctcgc catcggctgg tatatgctct
      181 atcgcaaccg cgagcacgta gaactgactg ccgttgagca agcttatctc aatgcaggta
      241 gcgtcaatgc ccgccgagat ccgctcagtt ttgccgaatg gcgcagcctg ttccgtaacc
      301 gtacaatgtg gggaatgatg ctcggattca gtggcatcaa ctacactgcg tggctgtatc
      361 tggcctggct tcctggttac ctgcaaacag cctataacct ggatttaaaa agcacagggt
      421 tgatgacggc tatccctttc ctgtttgggg ctgccgggat gctggtcaac ggttacgtta
      481 ccgactggct ggtcaaaggg ggaatggctc cgattaaaag ccgtaagatc tgcattattg
      541 ccgggatgtt ctgttctgcc gcctttacgc tgatagtacc acaagcgaca acatccatga
      601 cggcggttct gctgattggc atggcactgt tctgtattca ctttgccgga acatcctgcc
      661 ggggcttgat ccacgtcgca gttgcttctc gcatgactgc gtcggtgggc agtatccaga
      721 actttgccag cttcatctgc gcctcttttg cgccgatcat tactggtttt attgttgata
      781 ccacccactc attccgtctg gcactaatca tctgcggttg cgtcaccgca gcgggggcac
      841 tggcgtacat cttcctggtt cgtcagccga tcaacgaccc acgtaaagat taatctcctt
      901 atgcccgata acattttgct tatcgggcat tttcatggcg attaaaatgt ttcccagttc
      961 tcctcgctat ctgccacggc cattttacgc ggcgcagctg gcgtcacggt ttttaccaca
     1021 gccgatgttt cacgctgctg ttgctgaatc cggaacactg ccactgcttc ggtcaggcga
     1081 ctggcctgct cttccagcgc ggcggcggca gcggcagact cttccaccag cgcggcgttc
     1141 tgttgagtta cccggtccat ctcagcaacc gctaagccaa cctgatcgat accacggctc
     1201 tgctcatcag aagcagaagc aatttcgccc ataatgtccg tcacgcgggt cacggcgctg
     1261 acaatctccg ccattgtttc cccggcgctt tcgaccagcg tagagccaac atccactttc
     1321 cccaccgagt cttcaatcag gcttttaatt tcacgagccg cctgggcgct gcgctgggcc
     1381 agattacgca cttctcccgc gaccaccgca aaaccgcgcc cttgctcacc cgcacgcgca
     1441 gcctcaaccg ccgcgttcaa agccagaata ttggtctgga aggcaatgcc gtcaattacg
     1501 ctgataatat cggcgatttt ctgcgaactg gtggagatat cgcgcatagt ctgcaccacg
     1561 ttatctacca ctttgccgcc gcgttgcgcc gtttcagaag cacttaacgc cagatggctg
     1621 gcctggcgcg cattttcggc gttctgcttc accgttgcgg tcagttgctc catgctggct
     1681 gccgtctctt ccagcgaagc ggcctgttgc tcggtgcgcg aagagagatc gttattgccg
     1741 gtggcgattt cgctggcacc gctatagatg gcattggccc cgttgcgcac atcaccgacg
     1801 gtacgcatca gctctccctg catatggcgc aaactctctg ccagttgccc catctcatta
     1861 gagccatcca cctcaatcgg tttcaccaga tcgccgcctg caatatgacg aatgctgtca
     1921 atcaggcgat tcattggcgc taccagcgag gctttaatac cgaaccagac ggcgaagatg
     1981 accgccagta cgacgatcat cacgcccacc agaatccaca tcgcctggct gtaggaggca
     2041 ttgttatcgc tgacggcgat atcatagagc cgatcgtttt gctccatgta agccacatac
     2101 tgcttctcga aaccgtcctg atatccctgg gtcggctgat caaagaactc gttgattttg
     2161 cctgcaccta acagttggat cagctccgcc agcgcattgt gataaatatc gtaattacgt
     2221 ttgatctctg ccgctgcggc ggtgctctga cgcgggtcac gcggcaacgc ttcgtaatcc
     2281 gcccagtttt tttccgcctg tttcagcgaa atactggcac tctgcatcag ctcagcaacg
     2341 gttgaaccgc taccaatatt attctgatcc atcatgtagc ggatacccgc gcggttgagg
     2401 gtgttacgcg tctgcaacaa cgcgacccag ctgccattca gcgtggattg ctgctggcga
     2461 atggtttgta aaacagtgaa attttctttg tcattcttta aggcattaaa gaacagaccg
     2521 cctgatgtca gttgtaaaag gccaaaaacg gccaaaacca gcagtaagct ggtcacgatc
     2581 ttgatacgtt ttaacatggt ttctctttcc tgtggaccga tgcaagattt tcggcttgga
     2641 aagcaaaaac tttatgaaaa ggtgtaaccc gccatccatg gaaaaaccag gcgctggtta
     2701 tcagatacta aaaagttatt cattatcgcc cgggatggct tcttgtcggg aattgcccag
     2761 agcaataaat tcctctaaca gatgggttaa ctgttgcatt ttctcggcag taaactgagc
     2821 ttcaatctgc cgataggctt cttcaatctg tgtctgagcg cggttataca gcgcctgccc
     2881 ctctttggtt agcgagatat agagcttccg ctgatcattg atcggcttta atcgcaacac
     2941 taaaccgtcg cgttccatcc gcgtgagaat cccggtcagg cttgggcgca aaatgcaggc
     3001 gcgatacgcc agatcgtgaa aatccattga tgggctttcc gccagaatac gcacgatgcg
     3061 ccactgttgc tcagtcaaat tatgccgttt aacgattggg cgaaaataac tcatcgccgc
     3121 ttctcgcgcc tggagcaacg caatggttag tgagtcgtgc attatctttc ccctgaaaaa
     3181 ttcaaaatca ttaatataga aacagttaat cgtcacgtta ccgggactgc gctgcccctg
     3241 caattcctga ttttatcaaa attgatgata ttttattaac ttactgatat ttaatatttt
     3301 attatcttta aatgtaaata gtttgttaat tagatcacat ttacatcact tacttttgcc
     3361 aaactacgca atcgggaata aaagcaatca ttaacatatt aatgattaag ataatcgaat
     3421 ccgaggagtg gtaatgaaag gcactatctt cgccgtagcg ttgaaccatc gcagccaact
     3481 tgatgcatgg caggaagcgt tccagcaatc cccctacaaa gccccgccta aaactgcggt
     3541 ctggtttatt aaaccgcgca atacggtgat tggttgcggt gaaccgattc cctttccaca
     3601 gggtgaaaag gtactgagcg gtgcgactgt tgcgctgatt gtgggaaaaa cggcgacgaa
     3661 agtacgtgaa gaagatgcgg cagagtacat cgccggatat gcgctggcta acgacgtcag
     3721 cctgccggaa gagagctttt accgcccggc aatcaaagca aaatgtcgtg atggattctg
     3781 ccccattggc gaaaccgtgg ctctcagcaa tgtcgataat ctgaccatct ataccgagat
     3841 caacgggcgt cctgccgatc actggaatac cgccgattta caacgtaacg ccgcgcagtt
     3901 gctgagcgcc ctgagcgaat ttgccacgct gaatccaggc gacgccattc tgctcggcac
     3961 gccacaggcg cgcgtggaaa tacagccagg cgatcgcgtt cgtgttctcg cagaaggttt
     4021 cccgccgctg gaaaatccgg tagtggacga acgtgaagtg accacgcgca agagcttccc
     4081 aacgcagcca cacccgcacg gcacgctgtt tgccctcggc ctgaactacg ccgaccacgc
     4141 cagcgaactg gaatttaagc caccggaaga accgctggta ttcctgaaag cgccaaatac
     4201 cctcactggc gataaccaga cctccgtgcg tccaaacaat attgaataca tgcactatga
     4261 agcggagctg gtggtagtta ttggcaaaca ggcgcgtaac gtcagcgaag ccgatgccat
     4321 ggattatgtc gcgggctaca ccgtgtgtaa cgactacgcc attcgtgact atctggaaaa
     4381 ctactaccgc cctaacctgc gggtaaaaag ccgcgacgga ctgacgccga tgctttcaac
     4441 catcgtgccg aaagaggcga tcccggaccc gcataatctg acccttcgca ccttcgtcaa
     4501 cggcgagtta cgccagcaag gcaccaccgc cgatctgatc ttcagcgtgc ccttcctgat
     4561 cgcctactta agtgaattta tgaccctgaa tccgggcgac atgatcgcca ccggcacacc
     4621 caaaggctta tctgacgtgg tgcctggcga tgaagtagtg gtggaagtag aaggcgtggg
     4681 ccgcctggtg aaccgaattg tgagtgagga tacagcgaaa tgaaaaaagt aaatcattgg
     4741 atcaacggca aaaatgttgc aggtaacgac tacttccaga ccaccaatcc ggcaacgggt
     4801 gaagtgctgg cggatgtggc ctctggcggt gaagcggaga tcaatcaggc ggtagcggca
     4861 gcgaaagagg cgttcccgaa atgggccaat ctgccgatga aagagcgtgc gcgcctgatg
     4921 cgccgtctgg gcgatctgat cgaccagaac gtgccagaga tcgccgcgat ggaaaccgcg
     4981 gacaccggcc tgccgatcca tcagaccaaa aatgtgttga tcccacgcgc ttcccacaac
     5041 tttgaatttt tcgcggaagt ctgccagcag atgaacggca agacctatcc ggttgacgac
     5101 aagatgctca actacacgct ggtgcagccg gtgggcgttt gtgcgctggt atcgccgtgg
     5161 aacgtaccgt ttatgaccgc cacatggaag gtcgcgccgt gtctggcgct gggcaatacc
     5221 gcggtactga aaatgtcgga actctccccg ctgaccgctg accgcctggg tgagctggcg
     5281 ctggaagccg gtattccggc aggcgtgctg aacgtggtac agggctacgg cgcaaccgca
     5341 ggggatgcgc tggttcgtga tcatgacgta cgtgccgtgt cgttcaccgg cggtacggcc
     5401 accgggcgca acatcatgaa aaacgccggg ctgaaaaaat actccatgga actgggcggt
     5461 aaatcgccgg tgctgatttt tgaagatgcc gatattgaac gcgcgctgga cgccgccctg
     5521 ttcaccatct tctcgatcaa cggcgaacgc tgcaccgccg gttcacgcat ctttattcag
     5581 caaagcatct acccggaatt cgttaaacgc tttgccgaac gcgccaaccg tctgcgcgtg
     5641 ggcgatccga acgatccgaa tacccaggtt ggcgcgctta tcagccagca gcactgggaa
     5701 aaagtctccg gctatatccg tctcggcatt gaagaaggcg ccaccctgct ggcgggcggc
     5761 ccggataaac cgtctgacct gcctgcacac ctgaaaggcg gcaacttcct gcgcccaacg
     5821 gtgctggcag acgttgataa ccgtatgcga gtcgcccaag aagagatttt cgggccggtc
     5881 gcctgcctgc tgccgtttaa agacgaagct gaaggcttac gcctggcaaa cgacgtggag
     5941 tacggcctcg cgtcgtacat ctggacacag gatgtcagca aagtgttacg cctggcgcgt
     6001 ggcattgaag ctggcatggt gttcgtcaac acccagaacg tgcgtgacct gcgccagcca
     6061 tttggcggcg taaaagcctc cggcaccggg cgtgaaggcg gtgagtacag cttcgaagtg
     6121 ttcgcggaaa tgaagaacgt ctgcatttcc atgggcgacc atccaattcc gaaatgggga
     6181 gtctgatatg ggtaagttag cgttagccgc caagatcacg cacgtaccgt cgatgtatct
     6241 ctctgaactg ccagggaaaa accacggttg ccgccagggc gcgatcgacg ggcataaaga
     6301 gatcagcaag cgttgccggg aaatgggcgt cgataccatt atcgttttcg atacccactg
     6361 gctggtcaac agtgcttatc acatcaactg tgcagaccat tttgaaggcg tctacaccag
     6421 caacgagctg ccgcatttta tccgcgatat gacctacaac tacgagggca acccggagtt
     6481 ggggcagctt attgccgatg aagccttaaa gctcggcgtg cgggcaaaag cgcacaacat
     6541 tcccagcctg aaactggaat acggcacgct ggtgccgatg cgctacatga atgaagacaa
     6601 gcacttcaaa gtggtctcca tttcggcttt ctgcacggtt cacgattttg ccgacagccg
     6661 caagctgggc gaagcgattc tgaaagcgat cgaacagtac gacggcaccg tggcggtcct
     6721 tgccagcggt tcgttatcgc accgctttat tgacgatcag cgtgcagaag aagggatgaa
     6781 cagctacacc cgcgagttcg accgccagat ggacgagcgc gtggtgaagt tgtggcgcga
     6841 aggccagttc aaagagttct gcaatatgct gccggagtac gccgactact gctacggcga
     6901 aggcaatatg cacgacacgg tgatgctgct ggggatgctc ggctgggata aatacgacgg
     6961 caaggtggag tttatcaccg agctattccc aagctctggc accggtcagg ttaacgctgt
     7021 tttcccgctt cccgcgtaag gacgttttat gccgcacttt atcgttgaat gcagtgacaa
     7081 catccgcgaa gaagccgacc tgccggggtt gttcgccaaa gtgaatccga cgctggcagc
     7141 cacgggtatt tttccgctgg cgggtattcg cagccgcgtg cattgggtcg atacctggca
     7201 gatggccgac gggcagcgtg attatgcctt cgtgcatatg acgttgaaaa tcggcgcagg
     7261 tcgcagcctg gaaagccgtc agcaggcggg tgaaatgctg tttgaactga ttaaaacgca
     7321 cttcgccgcc ctgatggaga gccgcctgct ggcgttgtcg tttgagattg aagagctgca
     7381 tccgacgctg aattttaaac aaaacaacgt gcacgcattg tttaagtagc gcgcagattg
     7441 cccggtggcg ctgcgcttac cgggcctaca aaaccccaaa ccgtacaccg taggccggat
     7501 aaggcgcagc cgcatccggc aatgccacag gatatcgcta tgttcgataa acacacccac
     7561 accctgatcg cccagcgtct ggatcaggca gaaaaacagc gcgaacagat ccgcgcgatc
     7621 tcgctggatt acccagagat caccatcgaa gacgcttacg cggtgcagcg cgaatgggtt
     7681 cgcctgaaaa tcgccgaagg ccgtacgctg aaaggccaca aaatcggtct gacctcaaaa
     7741 gcaatgcagg ccagctcgca gatcagcgaa ccagattacg gtgccctgct ggatgacatg
     7801 ttcttccacg atggcagcga tatcccgacc gatcgcttta tcgtgccgcg cattgaagtg
     7861 gagctggctt tcgtgctggc aaaaccgctg cgtgggccaa actgcacgct gttcgacgtc
     7921 tacaacgcca cggactacgt gatcccggcg ctggagctga tcgacgctcg ctgccacaac
     7981 atcgatccgg aaacccagcg cccgcgcaaa gtgttcgaca ccatttctga taacgccgcc
     8041 aacgccgggg tgatcctcgg tggtcgtccg atcaaaccag acgaactgga tctgcgttgg
     8101 atctccgccc tgatgtatcg caatggcgtg attgaagaaa ccggcgtcgc cgctggcgtg
     8161 ctgaatcatc cggcaaacgg cgtggcctgg ctggcgaaca aactcgcacc gtatgacgta
     8221 caactggaag ccgggcaaat cattctcggt ggttcgttca cccgcccggt tccggcgcgt
     8281 aagggcgaca ccttccacgt cgattacggc aacatgggtt ccattagctg ccgctttgtt
     8341 taaggagaga acgatggaaa acagctttaa agcggcgctg aaagcaggcc gtccgcagat
     8401 cggattatgg ctggggctga gtagcagcta cagcgcagag ttactggccg gagcaggatt
     8461 cgactggttg ttgagcgacg gtgagcacgc gccgaataac gtgcaaaccg tgctcaccca
     8521 gctacaggcg attgcgccct accccagcca gccggtagta cgtccgtcgt ggaacgatcc
     8581 ggtgcaaatc aaacaactgc tggacgtcgg cacacaaacc ttgctggtgc cgatggtaca
     8641 aaacgctgac gaagcccgtg aagcggtacg cgccacccgt tatccccccg ccggtattcg
     8701 cggtgtgggc agtgcgctgg cccgcgcctc gcgctggaat cgtattcctg attacctgca
     8761 aaaagccaac gatcaaatgt gcgtgctggt gcagatcgaa acgcgtgagg caatgaagaa
     8821 cttaccgcag attctggacg tggaaggcgt cgacggcgtg tttatcggcc cggcggatct
     8881 gagcgccgat atgggttatg ccggtaatcc gcagcacccg gaagtacagg ccgccattga
     8941 gcaggcgatc gtgcagatcc gtgaatcggg caaagcgccg gggatcctga tcgccaatga
     9001 gcaactggca aaacgctatc tggaactggg cgcgctgttt gtcgccgtcg gcgttgacac
     9061 caccctgctc gcccgcgccg ctgaagcgct ggcagcacgg tttggtgcgc aggccaccgc
     9121 cgtgaagccc ggcgtgtatt aatgccggat gcgctgcact tatccggcct acactcgcac
     9181 caaaccgtag gccggataag gcgtttacgc cgcatccggc aaaaagccgt accaaatcgc
     9241 ggaccggata aggcgttacg ccgcatccgc cataaacctt gtcgtaccct acaaaaatcc
     9301 cattagagga agaaaaatga gcgacacctc acctgccata ccggagagta tcgatccggc
     9361 gaatcagcat aaagcgctga ctgccggaca acaggcggtt attaagaagc tatttcgccg
     9421 cctgatcgtc tttctgttcg tgctgtttat cttctcgttc cttgatcgca tcaacatcgg
     9481 ctttgccgga ctcacgatgg gacgcgacct cggtctgagc gccaccatgt ttggcctcgc
     9541 taccaccctg ttctatgccg cttatgtcat cttcggcatt cccagcaaca ttatgctgag
     9601 tattgtcggt gcgcggcgct ggatcgccac catcatggtg ctctggggca tcgcctctac
     9661 tgccaccatg tttgccactg gccccaccag cttgtacgta ctgcgtatac tggttggcat
     9721 taccgaagcc ggctttctgc ctggtattct gctgtattta accttctggt ttccggccta
     9781 tttccgcgcc cgtgccaacg ccttgtttat ggtagcaatg ccggtaacga cagcgttggg
     9841 ctcgatcgtt tccggctaca ttttgtcgct ggatggcgtc atggcattaa aaggctggca
     9901 gtggctgttt ttgctggaag gcttcccgtc ggtattactc ggcgtcatgg tgtggttctg
     9961 gcttgatgac tcaccggaca aagctaagtg gctgacgaaa gaagacaaaa aatgcctgca
    10021 agagatgatg gataacgatc gtctgacgct ggttcagcca gagggagcca tcagccacca
    10081 cgccatgcaa caacgcagta tgtggcggga gatcttcact ccggtggtga tgatgtatac
    10141 cctggcgtat ttctgcctga ccaacacact tagtgcgatc agcatctgga caccgcagat
    10201 cctgcaaagt tttaatcagg gcagcagtaa tatcaccatc ggcctgctgg ccgccgtacc
    10261 gcagatttgt accattctcg ggatggtcta ctggagccgt cactcagatc gccgccagga
    10321 acgaaggcat cacaccgccc ttccttattt gttcgctgcc gcaggttggt tactggcttc
    10381 ggcaactgat cacaacatga tccagatgct ggggatcatt atggcttcga ccggatcatt
    10441 cagcgcaatg gcgattttct ggacaacacc ggatcagtcc atcagcctgc gggcacgagc
    10501 gatcggtatt gcggtgatca acgccactgg caacattggt tcagcgttaa gtccgtttat
    10561 gatcggctgg ttgaaagatt tgaccggcag ctttaacagt ggattgtggt ttgttgccgc
    10621 gctgctggtg attggtgcgg ggattatctg ggcgattcca atgcagtcct cccgtccgcg
    10681 agcgaccccg taaggaacga cgatgtgtga ccgtcagatt gccaatattg atatcagcaa
    10741 agagtacgat gaaagcctgg gcacggacga tgtgcattat cagtcgttcg cccgcatggc
    10801 ggcatttttt ggccgccata tgctgccaca tcgccacgaa cagtactttc agatgcattt
    10861 cctcaatagc ggacagattg agctacagct tgacgatcat cgctactcgg tggaagcgcc
    10921 cctgtttgtc ctgacgccgc cgtcagtacc tcatgcgttt attacggagt ctgacgccga
    10981 cggtcatgtg ttgacggtac gggaagatct gatctggccc ctgctggaag ttctttatcc
    11041 gggcactcgg gaaaccttcg gcctgccggg ggcctgtcac tggcagataa acccgacgaa
    11101 ctggcggcgc tggaacacta ttggcaactg atagagcggg aatcggtgga acaactgcct
    11161 ggacgggaac acaccctgac gttactggca caggcagtgt tcaccctact gctgcgtaac
    11221 gcaaaactcg acgaccatgc cgccagcggg atgcgcggag aattaaaact gttccagcgt
    11281 tttaatatgc tgattgaaag ccattttcat cagcactgga cagtaccgga ttacgctaac
    11341 gaactgcata tcaccgaatc acgactcacg gacatctgcc gtcgctttgc caaccgtccg
    11401 ccaaaacggt tgattttcga caggcagctg cgagaagcca agcggctgct gctgttttct
    11461 gataacgccg tgaacaatat tgcctggcaa ctcggtttta aggatccggc ttattttgcg
    11521 cgctttttta atcgcttagt cggttgctcg cccagtgctt atcgcgccaa aaaagtacct
    11581 gtgacgtgaa aatcccttag ccttaacggg aaaccaggca ccacctgcta ttcccctttc
    11641 tccgcctgga gcaaggggat ttagtaacgt caaaaatcgc taaaagcgac ctcgatcaca
    11701 aaaccgtacc cgacctgaaa agtaccagcc attgtcccaa aagtctcttc ccgtaatcgc
    11761 ccctgcaagc tttaattgaa taacaaaaac aacacacaaa attaacaatc gtattccgat
    11821 taatactgta gaggtcgaca tgaaaccaga agatttccgc gccagtaccc aacgtccgtt
    11881 caccggggaa gagtatctga aaagcctgca ggatggtcgc gagatctata tctatggcga
    11941 gcgagtgaaa gacgtcacta ctcatccggc atttcgtaat gcggcagcgt ctgttgccca
    12001 actgtacgac gcgctacaca aaccggagat gcaggactct ctgtgctgga acaccgacac
    12061 cggcagcggc ggctataccc ataaattctt ccgcgtggcg aaaagtgcca acgacctgcg
    12121 ccagcaacgc gatgccatcg ctgagtggtc acgcctgagc tatggctgga tgggccgtac
    12181 cccagactac aaagctgctt tcggttgcgc actgggcgcg aatccgggct tttacggtca
    12241 gttcgagcag aacgcccgta actggtacac ccgtattcag gaaactggcc tctactttaa
    12301 ccacgcgatt gttaacccac cgatcgatcg tcatttgccg accgataaag taaaagacgt
    12361 ttacatcaag ctggaaaaag agactgacgc cgggattatc gtcagcggtg cgaaagtggt
    12421 tgccaccaac tcggcgctga ctcactacaa catgattggc ttcggctcgg cacaagtgat
    12481 gggcgaaaac ccggacttcg cactgatgtt cgttgcgcca atggatgccg atggcgtaaa
    12541 attaatctct cgcgcctctt atgagatggt cgcgggtgct accggctcgc catacgacta
    12601 cccgctctcc agccgcttcg atgagaacga tgcgattctg gtgatggata acgtgctgat
    12661 cccatgggaa aacgtactga tctaccgcga tttcgatcgc tgccgtcgct ggacaatgga
    12721 aggcggtttt gcccgtatgt atccgctgca agcctgtgtg cgcctggcag tgaaattaga
    12781 cttcattaca gcactgctga aaaaatcact cgaatgtacc ggcaccctgg agttccgtgg
    12841 tgtacaggcc gatctcggtg aagtggtggc gtggcgcaac accttctggg cattgagtga
    12901 ctcgatgtgt tctgaagcga cgccgtgggt caacggggct tatttaccgg atcatgccgc
    12961 actgcaaacc tatcgcgtac tggcaccaat ggcctacgcg aagatcaaaa acattatcga
    13021 acgcaacgtt accagtggcc tgatctatct cccttccagt gcccgtgacc tgaacaatcc
    13081 gcagatcgac cagtatctgg cgaagtatgt gcgcggttcg aatggtatgg atcacgtcca
    13141 gcacatcaag atcctcaaac tgatgtggga cgccattggc agcgagtttg gtggtcgcca
    13201 cgaactgtat gaaatcaact actccggtag ccaggatgag attcgcctgc agtgtctgcg
    13261 ccaggcacaa agctccggca atatggacaa gatgatggcg atggttgatc gctgcctgtc
    13321 ggaatacgac cagaacggct ggactgtgcc gcacttgcac aacaacgacg atatcaacat
    13381 gctggataag ctgctgaaat aacgcagcag gaggttaaga tgcaattaga tgaacagcgc
    13441 ctgcgctttc gtgacgcgat ggccagcctg tcggcagcgg taaatattat caccaccgag
    13501 ggcgacgccg gacaatgcgg gattacggca acggccgtct gctcggtcac ggatacacca
    13561 ccatcgctga tggtgtgcat taacgccaac agtgcgatga acccggtttt tcagggcaac
    13621 ggtaagttgt gcgtcaacgt cctcaaccat gagcaggaac tgatggcacg ccacttcgcg
    13681 ggcatgacag gcatggcgat ggaagagcgt tttagcctct catgctggca aaaaggtccg
    13741 ctggcgcagc cggtgctgaa aggttcgctg gccagtcttg aaggtgagat ccgcgatgtg
    13801 caggcaattg gcacacatct ggtgtatctg gtggagatta aaaacatcat cctcagtgca
    13861 gaaggtcacg gacttatcta ctttaaacgc cgtttccatc cagtgatgct ggaaatggaa
    13921 gctgcgattt aagctccagc cccttcacgt attcgtcgaa ggggcatttt tattgtgatc
    13981 tcaatcacat atccacccga tgaataattc ttgcccaacc gctcaccgcc attcctgacc
    14041 gcttacgtcc ctaaaataac cactcagtta tttaccttac tttacgcgcg cgtaactctg
    14101 gcaacatcac tgcaggatag cggtcaattt acctcctcaa acgcaacgca aacctagaac
    14161 ggcttcggcc tatgattaac aaattactct atgaattttg gtttgcagga aggcagcaag
    14221 tgagtgaatc cccgggagct tacaacagta agtgacagag gtgaacgaac gcagctgccg
    14281 cacctgtaag ccaaaagacg acgagtaaaa tgccaggttt tactatggat acgaaaaaac
    14341 tattcaagca cataccctgg gtgattctcg gaatcatcgg tgcattctgc ctcgcggtag
    14401 ttgcattacg tcggggggag cacgtcagcg ccctgtggat cgtggtcgcc tctgtgtcgg
    14461 tgtatctggt ggcgtatcgc tactacagtc tgtacatcgc ccagaaggtg atgaaactcg
    14521 accccacgcg cgcgacgcct gcggttatta acaacgacgg tctgaactac gttccgacca
    14581 accgttacgt gttgtttggt caccacttcg ccgctatcgc cggtgccggt ccgctggttg
    14641 gtccggtgct cgccgcgcag atgggctacc tgcctggcac gctgtggctg ctggcggggg
    14701 tagtactggc cggtgcggtt caggacttta tggtgctgtt tatctcctct cgccgtaacg
    14761 gcgcatctct cggtgagatg atcaaagaag agatgggacc agtaccgggg actatcgcgc
    14821 tgtttggctg tttcttaatc atgatcatca tcctcgccgt tctggcgctg attgtggtta
    14881 aagccctggc cgaaagtccg tggggtgtct tcaccgtttg ctcaaccgta ccgattgcgc
    14941 tgtttatggg tatctacatg cgctttatcc gtccggggcg tgtaggtgaa gtttctgtta
    15001 ttggtatcgt gctgctggtt gcctctatct atttcggtgg cgtgattgct cacgatccgt
    15061 actggggtcc ggcactgacc tttaaagaca ccaccattac cttcgcgctg attggctatg
    15121 cgtttgtttc cgcactgctg ccggtgtggc tgatcctcgc accgcgcgac tatctggcaa
    15181 ccttcctgaa aatcggcgtt atcgtcggcc tggcgctggg tatcgtggta ctgaacccgg
    15241 aactgaaaat gcctgcaatg acccagtaca ttgacggtac tggcccgctg tggaaaggcg
    15301 ctctgttccc gttcctgttc atcaccatcg cctgtggtgc ggtatctggc ttccacgcgc
    15361 tgatctcttc cggtacgacg ccgaaattgc tggctaacga aaccgacgcg cgtttcattg
    15421 gctacggcgc aatgctgatg gaatccttcg tggcgattat ggcgctggtt gctgcgtcca
    15481 tcatcgaacc gggtctttac ttcgcgatga ataccccgcc tgctggcctt ggcatcacca
    15541 tgcctaacct gcatgaaatg ggtggcgaga acgcgccgat catcatggcg cagctgaaag
    15601 acgttaccgc acacgcggcg gcgaccgtca gctcctgggg cttcgtgatt tcgccagagc
    15661 agatcctgca aaccgcgaaa gacattggtg agccttctgt cctgaaccgt gcaggtggcg
    15721 cgccgacgct ggcggtaggt atcgctcacg tgttccacaa agtgctgccg atggctgaca
    15781 tgggcttctg gtatcacttc ggtattctgt tcgaagccct gttcatcctg accgcgctgg
    15841 atgcgggtac tcgttctggc cgctttatgc tgcaagacct gctgggtaac ttcatcccgt
    15901 tcctgaaaaa aaccgactct ctggttgccg gtatcatcgg tactgcgggc tgtgtgggtc
    15961 tgtggggcta cctgctgtat cagggcgtgg tcgatccgct gggcggcgtt aagagcctgt
    16021 ggccgctgtt cggtatttcc aaccagatgc tggcagccgt agcgctggta ctgggcaccg
    16081 ttgtgctgat taagatgaag cgcacccaat acatctgggt aactgttgtt ccggctgtat
    16141 ggctgcttat ctgcaccacc tgggcgctgg gtctgaaact gttcagcacc aacccgcaga
    16201 tggaaggctt cttctacatg gcaagccagt acaaagagaa gattgctaac ggtactgacc
    16261 tgacggcgca gcagattgcc aacatgaacc acatcgttgt gaacaactac accaacgcag
    16321 gcctgagtat tctgttcctg attgtggtgt acagcatcat cttctacggt ttcaaaacct
    16381 ggcttgcggt gcgtaacagc gacaaacgta ctgacaaaga aactccgtac gttccaatcc
    16441 cggaaggcgg cgtgaagatc tcttcgcacc actaaccggt tgccggatgc gctgcgcgta
    16501 tccggcctac aaagtgtcta tcgtaggccg gataaggcgt ttacgccgca tccggcaaat
    16561 ccctagcccg gaccttccgg gctttacgca tatctggaag gcaaaatgtt tggtaactta
    16621 ggacaggcaa aaaaatatct cggccaggcg gcgaagatgt tgattggcat tccagactac
    16681 gacaactacg tcgagcatat gaagaccaac catcccgaca agccgtacat gagctatgaa
    16741 gaattcttcc gcgaacgcca gaacgcgcgc tacggcggcg atggtaaagg cggtatgcgc
    16801 tgttgttaat ggagagagca tgaacccgat tgcagttacc ctactcaccg gttttttagg
    16861 cgcgggcaaa accaccctgc tgcgccatat tcttaacgaa caacacggct acaagattgc
    16921 cgtgattgaa aacgaattcg gcgaagtctc cgttgatgat caactgattg gcgaccgcgc
    16981 cacgcagatc aaaacgctga ccaacggctg catctgctgt tcgcgctcca acgagctgga
    17041 agacgcacta ctcgacctgc tggataatct cgacaagggc aatattcagt tcgaccgtct
    17101 ggtcattgaa tgcaccggca tggccgatcc cggcccgatt attcagacct tcttctccca
    17161 tgagatttta tgccagcgct acctgctgga cggggtgatt gcgctggtgg atgcggtaca
    17221 tgccgatgag cagatgaacc agttcaccat cgcccagtcc caggttggct acgccgaccg
    17281 tattctgctg acgaaaaccg acgtcgcagg cgaagcagag aaactgcgtg aacgcctggc
    17341 gcgcatcaac gcccgcgcac cggtctacac cgttacccac ggcgatatcg acctgggcct
    17401 tctgttcaac accaacggtt ttatgctgga agaaaacgtc gtcagcacca aaccgcgttt
    17461 ccactttatc gcggacaaac aaaacgatat ttcgtcgatt gtggtggaac tggattaccc
    17521 ggtagatatc agcgaagttt cccgggtgat ggaaaacctg ctgctggaat cggcggataa
    17581 actgctgcgt tacaaaggga tgctgtggat tgacggcgaa cctaaccgcc tgctgttcca
    17641 gggcgtccag cgcctctaca gcgccgactg ggaccgcccg tggggcgatg aaaaaccgca
    17701 tagcacgatg gtgtttatcg ggattcagtt gccggaggat gagattcggg cggcgtttgc
    17761 gggactcaaa aagtaaccag ccctccaaca tcacaatacc tgagacttta tcaccctcat
    17821 attcctccag aatatgaggg cattcgggct taattatctc tgaacgtgca acacttccta
    17881 cgactcctta tgcgcctcca catcctgctt cgttaaccag agcgacttac gaccaaaatt
    17941 ttcgtacatt ttcagctgat cttcatagtg ctgatcaact gttccatcgg gagcaataaa
    18001 cccactctga ccgggtgcga ccacatccca ggcaagcacc ggacgatcgc ttgtcgttgg
    18061 tgagaaaaca atcatatcgt tttctgttcc acggttttga tactccgcct gatgacgcgt
    18121 ttcttccgct gcggcctgcg gtacatcaaa gaaattattt gcccggaacg ttaaggccat
    18181 tgcaggtgtt ttccagttac tcacattatt gccatagcgt ttggaaagag tctcccaggt
    18241 atcttccagc gcagccaaca caacctcctg ctgtggtttc ccagcaaaca gatcaaccgc
    18301 ctgtgggatt ggtgatttgt ctccctgcac cgcctcatac aaaatttttg ctccaacact
    18361 tatattcagc gaaccagttg ggccgtcctg ggttgtttcg tagccactgg cgctgtacca
    18421 cttatcaaat ggcataggta cggcagccac tacggtacgc ttcaacatac tggtcagcca
    18481 aacgttcagg atggcagagc ctggc
//
LOCUS       DADLFA010000073        17926 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000073 DADLFA010000000
VERSION     DADLFA010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17926
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.073"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..118)
                     /locus_tag="JQH65_002112"
     CDS             complement(<1..118)
                     /locus_tag="JQH65_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HAY6139520.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKDGNKVDFYGKMV"
     gene            679..1044
                     /locus_tag="JQH65_002113"
     CDS             679..1044
                     /locus_tag="JQH65_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAY6139521.1"
                     /translation="MEKCDFYHIIVLSLNFPGYLKMEYGSTKMEERLSRSPGGKLALW
                     AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLGALVGFLFMALVGAL
                     LGSIAWYTRPRPARSRRYE"
     gene            1084..1779
                     /locus_tag="JQH65_002114"
     CDS             1084..1779
                     /locus_tag="JQH65_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAY6139522.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCH
                     VIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            1846..3264
                     /locus_tag="JQH65_002115"
     CDS             1846..3264
                     /locus_tag="JQH65_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAY6139523.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMAKGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            3245..3715
                     /locus_tag="JQH65_002116"
     CDS             3245..3715
                     /locus_tag="JQH65_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HAY6139524.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGSPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(3704..4624)
                     /gene="yedA"
                     /locus_tag="JQH65_002117"
     CDS             complement(3704..4624)
                     /gene="yedA"
                     /locus_tag="JQH65_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HAY6139525.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLMAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            4797..5714
                     /locus_tag="JQH65_002118"
     CDS             4797..5714
                     /locus_tag="JQH65_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HAY6139526.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDSLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKIRGQAH"
     gene            5793..5975
                     /locus_tag="JQH65_002119"
     CDS             5793..5975
                     /locus_tag="JQH65_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HAY6139527.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            6146..7840
                     /gene="yedQ"
                     /locus_tag="JQH65_002120"
                     /pseudo
     CDS             6146..7840
                     /gene="yedQ"
                     /locus_tag="JQH65_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
     gene            complement(7837..8652)
                     /locus_tag="JQH65_002121"
     CDS             complement(7837..8652)
                     /locus_tag="JQH65_002121"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HAY6139528.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGVPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(8950..9166)
                     /gene="yodD"
                     /locus_tag="JQH65_002122"
                     /pseudo
     CDS             complement(8950..9166)
                     /gene="yodD"
                     /locus_tag="JQH65_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
     gene            9275..9517
                     /gene="dsrB"
                     /locus_tag="JQH65_002123"
     CDS             9275..9517
                     /gene="dsrB"
                     /locus_tag="JQH65_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HAY6139529.1"
                     /translation="MPEKRNCSTLAQSHRRKTMKVNDRVTVKTDGGPRRPGVVLAVEE
                     FSEGTMYLVSLEDYPLGIWFFNEAGHQDGIFVEKAE"
     gene            complement(9561..10184)
                     /gene="rcsA"
                     /locus_tag="JQH65_002124"
     CDS             complement(9561..10184)
                     /gene="rcsA"
                     /locus_tag="JQH65_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HAY6139530.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSNKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(10469..11254)
                     /gene="fliR"
                     /locus_tag="JQH65_002125"
     CDS             complement(10469..11254)
                     /gene="fliR"
                     /locus_tag="JQH65_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HAY6139531.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFVIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            complement(11263..11532)
                     /gene="fliQ"
                     /locus_tag="JQH65_002126"
     CDS             complement(11263..11532)
                     /gene="fliQ"
                     /locus_tag="JQH65_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HAY6139532.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            complement(11542..12278)
                     /gene="fliP"
                     /locus_tag="JQH65_002127"
                     /pseudo
     CDS             complement(11542..12278)
                     /gene="fliP"
                     /locus_tag="JQH65_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly.; frameshifted; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
     gene            complement(12278..12643)
                     /gene="fliO"
                     /locus_tag="JQH65_002128"
     CDS             complement(12278..12643)
                     /gene="fliO"
                     /locus_tag="JQH65_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HAY6139533.1"
                     /translation="MNNHATVQSSTPVSAAPLLQVSGALIAIIALILAAAWQVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            complement(12646..13059)
                     /gene="fliN"
                     /locus_tag="JQH65_002129"
     CDS             complement(12646..13059)
                     /gene="fliN"
                     /locus_tag="JQH65_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HAY6139534.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMHRLSR"
     gene            complement(13056..14060)
                     /gene="fliM"
                     /locus_tag="JQH65_002130"
     CDS             complement(13056..14060)
                     /gene="fliM"
                     /locus_tag="JQH65_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HAY6139535.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            complement(14065..14529)
                     /gene="fliL"
                     /locus_tag="JQH65_002131"
     CDS             complement(14065..14529)
                     /gene="fliL"
                     /locus_tag="JQH65_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HAY6139536.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALNTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            complement(14634..15761)
                     /gene="fliK"
                     /locus_tag="JQH65_002132"
     CDS             complement(14634..15761)
                     /gene="fliK"
                     /locus_tag="JQH65_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HAY6139537.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLISDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADQNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            complement(15758..16201)
                     /gene="fliJ"
                     /locus_tag="JQH65_002133"
     CDS             complement(15758..16201)
                     /gene="fliJ"
                     /locus_tag="JQH65_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HAY6139538.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERLSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            complement(16220..17588)
                     /gene="fliI"
                     /locus_tag="JQH65_002134"
                     /pseudo
     CDS             complement(16220..17588)
                     /gene="fliI"
                     /locus_tag="JQH65_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
     gene            complement(17588..>17926)
                     /gene="fliH"
                     /locus_tag="JQH65_002135"
     CDS             complement(17588..>17926)
                     /gene="fliH"
                     /locus_tag="JQH65_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HAY6139539.1"
                     /translation="ASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
BASE COUNT         4399 a         4485 c         4715 g         4327 t
ORIGIN      
        1 caaccatctt gccgtagaaa tcaactttat tgccatcttt attgtaaatt tcagccgcat
       61 ttgctgcgcc agcaactaat aacgccggga ccagcattgc cagaactttt cttttcatta
      121 tgtattccct tgtgattata atcttcatga atatatcaat aagtgccgtt atccaaaaaa
      181 aagcacattt ggatactatt ctatgaagtt catttcattt taagtattac atgcaacaaa
      241 tgtatttaaa agatttcaaa atgtttctaa tctatttaat ataattcaga gatatgaatt
      301 ataaatattc atgaaaatta taaatgtata tcaatatgtt atgagttgag cggaaacata
      361 gggggtatca atgattgttg cgccaaacgg ctggtgtgat caggcgcaca ttaatgaagg
      421 cataaaaaaa agccggcatc atgccggccc aaaaaatata aacaagacga taaaaggcta
      481 ataacggaag catcatgaca cagaataata aaaaggtata attgtaatca gtgctaatat
      541 attgattaaa tgaattaata aagccgatat caaagataaa taaaatcgct caaaacgatt
      601 tttcctgtat tcactgccgt tgcgcaaaat ttatctattt gttcaaaaaa tgattgagtc
      661 ttgactggct catccaatgt ggaaaaatgt gacttttatc acataatagt actaagtctg
      721 aattttccgg gttatctcaa aatggaatac ggttcgacaa agatggaaga gagactctct
      781 cgcagccctg gcggcaaact ggctttgtgg gctttctata catggtgtgg ctatttcgtc
      841 tgggcaatgg cgcgttacat ctgggtgatg agccggatcc ccgatgcccc cgtgtcggga
      901 tttgaaagcg atttaggctc taccgcggga aagtggttag gggcactggt cggattttta
      961 tttatggctc tggtcggggc attgttaggc agcattgcct ggtacacccg acctcgtccc
     1021 gcacgtagca gacgctatga ataaaaactg ctaaggtggc gactctttgg caacggagcg
     1081 cgtatggact tacaacactg gcaggcacag ttcgaaaact ggttgaaaaa ccatcatcag
     1141 caccaggacg cggcccatga cgtgtgtcat tttcgccgcg tctgggctac ggcacagaag
     1201 ctggcggcag atgacgatgt cgatatgctg gtgattttaa ccgcctgtta ttttcacgat
     1261 attgtgagcc tggcgaaaaa tcatccgcaa aggcagcgtt cttcaatcct ggcggcagaa
     1321 gagacgcgtc gcctgctccg tgaagagttc gagcaatttc cggcggagaa aatcgaggcc
     1381 gtttgtcatg tcattgccgc tcacagtttc agcgcgcaaa tcgccccctt aacaacggaa
     1441 gctaaaatcg tccaggatgc ggatcggctg gaagccctgg gggcgattgg cctggcgcgc
     1501 gtgtttgccg tttcaggggc attgggcgtg gcgctgtttg atggtgaaga tccgttcgca
     1561 cagcatcgcc cgcttgatga taaacgctac gcgctggatc atttccagac taagttgctg
     1621 aagctaccgc aaaccatgca aaccgcaaga ggcaagcagc tggcgcagca caacgcgcat
     1681 tttttagtcg agtttatggc gaagctcagt gccgaactgg cgggggagaa tgaaggtgtc
     1741 gatcacaagg tgatagatgc gttttcaccc gcgggataag cgcgtggccc taaatggctg
     1801 taattatgtt aacctgtcgg ccatctcaga tggccggtta aatctatgca ggaaaatata
     1861 tcagtaaccg attcatacag caccgggaat gccgcacagg caatgctgga gaaactgctg
     1921 caaatttatg atgttaaaac gctggtggcg cagcttaatg gtgtgggtga gaatcactgg
     1981 agcgcggcaa ttttaaaacg tgcgctggcg aatgactcgg catggcaccg tttaagtgag
     2041 aaagagttcg cccatctgca aacgttgtta cccaaaccac cggcacatca tccgcattat
     2101 gcgtttcgct ttatcgatct atttgccgga attggcggca tccgtcgcgg ttttgaatcg
     2161 attggcggac aatgcgtgtt taccagcgaa tggaacaaac atgcggtacg cacttataaa
     2221 gccaaccatt attgcgatcc ggcgacgcat cattttaatg aagatatccg cgatatcacc
     2281 ctcagccata aagaaggcgt gagtgatgag gcggcggcgg aacatattcg tcaacacatt
     2341 cctgaacacg atgttttact ggccggtttc ccttgtcagc cattttcgct ggctggcgta
     2401 tcgaaaaaga actcgctcgg gcgggcgcac ggttttgcct gcgataccca gggcacgctg
     2461 ttttttgatg tggtacgcat tatcgacgcg cgtcgtccgg cgatgtttgt gctcgaaaac
     2521 gtcaaaaacc tgaaaagtca cgaccagggt aaaacgttcc gcatcatcat gcagacgctg
     2581 gacgaactgg gctatgacgt ggctgatgca gaagataacg ggccggacga tccgaaaatc
     2641 atcgatggta aacattttct gccgcagcac cgtgaacgca tcgtgctggt gggttttcgt
     2701 cgcgatctta atctgaaagc cgattttact ctgcgtgata tcagcgaatg tttccctgca
     2761 cagcgagtga cgctggcgca gctgttggac ccgatggtcg aggcgaaata tatcctgacg
     2821 ccggtgctgt ggaagtacct ctatcgatat gcgaaaaaac atcaggcgcg cggtaacggc
     2881 ttcggttatg gaatggttta tccgaacaat ccgcaaagcg tcacgcgtac gctgtctgcg
     2941 cgttattaca aagatggcgc ggaaatttta atcgatcgcg gctgggatat ggccaaaggt
     3001 gagaaagact ttgacgatcc gctgaatcag caacatcgtc cacgtcggtt aacgcctcgg
     3061 gaatgcgcgc gcttaatggg ttttgaagcg ccgggagaag cgaaattccg cattccggtt
     3121 tcggacactc aggcctatcg ccagttcggt aactcggtgg tcgtgccggt ctttgccgcg
     3181 gtggcaaaac tgcttgagcc aaaaatcaaa caggcggtgg cgttgcgtca gcaagaggca
     3241 caacatggcc gacgttcacg ataaggccac tcgcagcaaa aatatgcgcg cgattgccac
     3301 gcgtgatacg gcgattgaga agcgcctcgc cagtctgtta accgggcagg gcctggcgtt
     3361 tcgcgttcag gacgccagtc tgcccggaag cccggatttt gtcgttgatg aatatcgctg
     3421 cgtgatattt acccatggct gcttctggca tcatcatcac tgctatctgt ttaaagtgcc
     3481 tgcgactcga accgagttct ggctggagaa gataggtaaa aatgttgaac gcgatcgccg
     3541 cgatatcagt cgtttgcagg aactcggctg gcgcgtactg attgtctggg agtgtgcatt
     3601 acgtgggcgc gagaagctga cggatgaagc gcttaccgag cgtctggaag agtggatctg
     3661 cggcgaaggt gccagcgcgc agatcgacac gcaggggatt catttactcg cttgatgcgt
     3721 cctgaataac tggcgcaact acgggttttg ccgggaagag atattttccc aacgtgacca
     3781 gtaccaccgc gaagacaatt acgccgagcg ccagccattc aatcttcgac agcgtctctc
     3841 cgcccagtcc cgtacccagc aagaccgcga ccaccgggtt aacgtaggcg tagctggtgg
     3901 cgagagccgg actgacatta cggattaaat acatataagc gttgatggcg ataatcgaac
     3961 caaacagcgc cagatagccg accgcaagga agcctgaaag ggaagggagc gccgtcagtt
     4021 tttcacctgc aatcatcgac gcgatcatta acaccacgcc tgccgccagc atctcaatcg
     4081 cacccgccat catccctaca ggtaaggtaa tgcgcgagcc ataaaccgag ccaaacgccc
     4141 agctaatcga gccgattaaa atcagaatcg cgccccacgg attgccgctt aaatttccac
     4201 cgctattgag catgatgatt ccggcaagcc caatggcgat acccacccat tccaatttgc
     4261 gcgttttaat gccaaacagg cggctgaagc acagggtaaa gaggggcacg gttgcaacca
     4321 caacggcggc gatgccggaa ggaacatttt gatgctcggc aaccgtcacc atgccattac
     4381 cgacagccag caataacagg ccaatcagcg cggcattgag cagcggacgt agcgggggga
     4441 gcttgtgtcc gcgcagtagc aaaaatgcca tcaataaaat accggctgcc aggaatcgaa
     4501 cgcctgccat cattaacgga ggccagcttt ccacgccaat ccgaatgaca aaataggttg
     4561 agccccaaat gatatacaac gcaaacagcg cgccaaaaag cggtaacaac tggcggaaac
     4621 gcataatccc tcacggtgga aataaaaagg tggttcatag taaacgtgaa aatcattctg
     4681 ctggcgagag atataattgc acttgattgt taaaaaaatg ttgacctgtg aagcaagtat
     4741 cagagctgcc gtttttgctt catacttaca cccttcaaca ataaaaatga gagggaatgc
     4801 ttttggccgg gagtagttta ctgacgttgc tcgatgatat cgccacacta ctggacgata
     4861 tctccgtgat gggcaaactg gcggcgaaga aaaccgccgg tgtattaggg gatgacttat
     4921 cgctcaatgc gcaacaagtt tcaggcgtgc gggccaaccg ggaacttccc gtggtctggg
     4981 gcgtggcgaa aggatcgctg attaataaag tgattctggt gccgctggcg ctgatcatca
     5041 gtgcgtttat cccgtgggcg attacgcctc tgttgatgat tggtggcgcg tttctctgct
     5101 ttgaaggagt agagaaagtg ctgcatatgc tggaggcgcg taaacataaa gaagatccgg
     5161 cgcagagcca gcagcgtctg gagaagctgg cggcgcagga ttcgctgaag tttgaaaagg
     5221 acaaaataaa aggggcgatt cgtaccgatt ttatattgtc tgccgaaatc gtcgccatca
     5281 cgctggggat tgtggccgaa gcgccgttgc ttaatcaggt gctggtgctt tcaggcatcg
     5341 cgctggtagt gaccgtgggc gtctacggtc tggtaggggt tatcgttaag attgatgacc
     5401 tggggtattg gctggcggaa aaatccagcg cgctgatgca ggcattaggt aaagggttat
     5461 tgatcattgc gccctggctg atgaaagcgt tatcgattgt cggcacgctg gcgatgttcc
     5521 tcgtcggcgg cgggattgtg gtacatggta ttgcgccgct gcatcacgcc attgaacatt
     5581 tcgctgggca gcaaagtgca gtggtggcga tgatattacc gactgtttta aatctgattc
     5641 ttggatttat catcggcggc atcgtggtgc tgggagtgaa agccgtagcg aaaatacgcg
     5701 gtcaggcaca ttaatactcg cacaggctat tatgcaaaat tcgccatctt caactaaggt
     5761 gaacaagttt cactcgaaaa aaggaggcga gtatgagctt tatggttagt gaggaagtta
     5821 cggttaaaga gggcggcccg cggatgattg tcaccggata ctccagcggt atggttgagt
     5881 gtcgttggta tgacggttac ggggtcaagc gggaagcttt tcatgaaacc gagcttgttc
     5941 cgggggaggg gagtcgttct gcggaagaag tttgaaaggg tatcattaac accaggccgg
     6001 atcacttccg gcctggtgtg ccaatgcctg caatgaatga tagtcgcgtc ttatcatgcc
     6061 accattataa aattatcgct gttttgcggt acgctatcgc caggttatgc cagaatcata
     6121 aaaaagcagg ttgggagtcg tcagggtgca gcacgagaca aaaatggaaa accagagctg
     6181 gttgaaaaaa atcgcacgcc gcctggggcc tggtcatgtc gttaatctct gctttatcgt
     6241 ggtattgctt ttttccacct tgctgacctg gcgtgaagtg gtagtgctgg aagatgccta
     6301 tatctccagt cagcgtaatc atctggaaaa cgtagccaac gcgctcgata agcatttgca
     6361 gtataacgtc gacaaactga tctttttgcg taatggcatg cgcgaagctc tcgtagcgcc
     6421 actggatttc acttcactgc gtaatgctgt aaccgagttc gaacagcatc gcgacgagca
     6481 cgcctggcaa attgaactca accgacgacg caccctgtca gtcaatggcg tatcggatgc
     6541 attagtcagc gaggggaatc tcctgtctcg cgaaaatgaa agcctcgaca atgaaattac
     6601 cgctgcactg gaagttggtt acttgctgcg actggcgcac aacacctcgt cgatggttga
     6661 acaggcgatg tatgtctcgc gtgccggatt ttacgtttcg acgcagccga ccttgtttac
     6721 gcgcaatgta ccaacgcgtt attacggcta tgtcacccaa ccctggttta tcggccattc
     6781 gcaatgagaa aatcgtcacc gcgcggtacg ctggtttact tcgcaaccgg aacacgccag
     6841 caatactgaa ccgcaggtta ccgtcagtgt tccggtagac agtaataact actggtatgg
     6901 cgtgctgggg atgagtattc ccgtgcgtac catgcagcaa tttttaagaa acgccatcga
     6961 taaaaacctc gatggtgagt atcagctcta tgacagtaag ctgagatttt tgacctcttc
     7021 caatcctgac catccaacag ggaatatttt tgatcctcgt gaactggcct tgctggcgca
     7081 ggcgatggaa catgacacgc ggggcggcat tcgtatggac agtcgctatg ttagctggga
     7141 acgtctggac catttcgacg gtgtgctggt gcgcgtccat acgctaagcg aaggcgtgcg
     7201 cggcgatttc ggcagtatca gcattgcatt aaccctgctg tgggcgctct ttaccaccat
     7261 gttactcatc tcctggtatg tgattcgccg gatggttagc aacatgtatg ttctgcaaag
     7321 ctcgttgcag tggcaggcgt ggcacgacac cttaacccgt ttatataatc gtggcgcact
     7381 gtttgaaaaa gctcgtccgc tcgcgaaatt gtgtcagacg caccaacatc ctttttctgt
     7441 cattcaggtc gaccttgacc attttaaagc gattaatgac cgctttagtc atcaggcggg
     7501 cgaccgtgtt ctttctcatg ctgccggatt aattagcagt tccttgcgtg cgcaggacgt
     7561 tgccgggcgg gtcggtggtg aggagttttg tgtgattctg ccaggcgcga gtctgacgga
     7621 ggctgcggaa gtcgcagaac gtattcgcct gaagttaaat gaaaaagaga tgttgattgc
     7681 taagagtacg acgatacgca tcagtgcctc gttgggggta agtagcagcg aggaagccgg
     7741 tgattatgat tttgaacaac tccagtcact ggctgaccgt cggctttatc tcgctaaaca
     7801 ggccgggcgt aatcgggtat gcgcgagcga taacgcttaa cgagcggaga aaaaatggtc
     7861 cagcccttca cgccatcctt ccggtccttc acgctgcgtt cgccagacgc gggccggatc
     7921 ctcatcatgc agatgcaccc cttcacggtt tagccctttc acaatcaccg cgtaatccat
     7981 tacctccagt aagggcgcat cgtttggccc atcgcccagg ccaagtgtgg ttgggcgttt
     8041 gcctgataat tgttgatagg tcgcgataat ccagttggcg gcctgatctt ttccggcaga
     8101 ggcatccagg acgtgccaga agcgcgcacc ctgcataaac tgtaagccca gttcgttcag
     8161 acgagcggta aattgtgcca tacgctcgtc actgtcgcgc cagattagcg ttaccgacgc
     8221 ctcatgcagc tgcgtcagcg ccgcctggct acggcttaat cccgtccatt cggcgatggt
     8281 tgcatcgtcg acatcatcga aagtcgtgaa tttaaaatgt tctttctcgc gtagcgtatt
     8341 taaaacctgg ctgatttcgc catggctaat acctgagatg atgcgtggaa aaccgtctat
     8401 atcctgccat tgttcagcaa gctggatcac tgcgccattt tcggcaatca gtggtacccc
     8461 ttgtaacccc aacgtttttt gcaagtacag catttccgct gaagttttac tgctacagag
     8521 aatgacggga acattcgctt cgcgtaaacg gctgagccag ggggcagccg gttgccagtc
     8581 ataactatga ctgtccagca gggtgccatc aagatcgcta aaaaccagta gtggttgttg
     8641 aattgaaaac atggtcgcgc agttctcccc ttaccaggat gattctcaaa acagcacaaa
     8701 taatcaaaga tgattttttc gggagcgatc ccgttgacgc cagagccacc aaggggtgac
     8761 gcacgtcaca tttctattca taagtagcgt taatcattca tatcgcgaat attttcttgt
     8821 cagcgaaaaa aattgcggat aaggtgatga acacatcaga tttcctggtg taacgaattt
     8881 tttaagtgct tcttgcttaa gcaagtttca tcccgacccc ctcagggtcg ggattttttt
     8941 attgtgcatt caacgattca cttcagagac gctgaagtca tgaatatcca gttcgaaggc
     9001 atccgccaat tcacgccatg tatgatattc acgtccatcg acgctaacgt gttcagaatc
     9061 gttctggctg cgatgaactt atttcattga tcgccgccag cagggcatcg acatcgacgc
     9121 tgacctcacg ttttgcggta tcgctgtact cttttgcggt tttcatcatc tttcctcgca
     9181 accgttactc tgttgttgcc gccagtcgtt aagtccgcct ggcatctctt aagtatagac
     9241 cgtcgagaaa atcagcactc gcgcgcgcct ggcgctgcca gaaaaacgaa attgttctac
     9301 actggcacaa agccacagga ggaaaacgat gaaggtgaat gatcgggtaa cagtcaaaac
     9361 ggatggcggt ccgcgtcgtc ctggcgtggt actagcagtt gaggagttta gtgaaggcac
     9421 aatgtacctg gtttcgctgg aagactaccc gctcggcatc tggttcttta atgaagcagg
     9481 gcatcaggac ggtatctttg tggagaaagc agagtaatct tattcagcct gactggtggg
     9541 aaaccaccag tcagaatgtg ttagcgcatg ttgacaaaaa taccattagt cacattatcc
     9601 gtcagtcgga cgacatggta gataacctgt ttattatgcg ttttgatctt acgtttaata
     9661 ttacctttat tcgatgaaac ggtcttggct ttgatattca tttggtcaga gatttgaatg
     9721 gttccctgac ctgccatcca cattcgcaac atactcgatt cggttcggct caatgataac
     9781 gtcggcatat ttaaaaaaga ggttatcgtt gtctcttttt tcagaatatc gccaaggata
     9841 tcgtcgagag attccggttt aatcgattta gaacttatca ataaattttt tctgaccaat
     9901 agatattcat caaaatgaac attggcaatt gccataaaaa cgataaataa cgtattggga
     9961 tgttgattaa tgatgtgctt gatgtgctga ctgttagaag catcatggat gaaacagtcc
    10021 tcattaataa acaccactga agggcgctgt gaatcacaag ctatggcaag gtcatcaacg
    10081 gtttcaatgt cgttgatttc tcttttttta acccctctac tcaacagata cccggttaaa
    10141 cctagtcggg tgtaactaca taaatccata ataatcgttg acatggcata ccctcactca
    10201 atgcgtaacg ataattcccc ttacctgaat atttcatcat gactaaacgg aacaacatgg
    10261 gtcacctaat gcgccactct cgcgattttt caggcggact tactatcccg taaagtgttg
    10321 tataatttgc ctggaattgt cttaaagtaa atgttgcgat atgtgagtga gcttaaaaca
    10381 aatatttcgc cgcaggagta tcctggaaga tgttcgtagg tattattttt cggataatcc
    10441 ttaggataac atgataaacg ttacggaatt atattaatgg caattcacta ataatatcag
    10501 ccagcaaatt aaacatctca ctgaataaat gttcgcaaaa aggtgcaatt aacggcatta
    10561 atgccgccat taaagagata ccgacagtca gagttaatgg aaatccaata acaaaaatgg
    10621 ataattgcgg ggccatacga ttcagtaaac ctaatgccag attcagggtc agcagcagag
    10681 taatgagcgg taacgccagc atcagcccgt taaggaaaat caaactcccc gctttggtga
    10741 gtgccagaaa cgcattgctg ttcaacggtt cgccaccaat tggcagggtg tgaaaggtat
    10801 cgaccagcag tgaaatcaac cataaatgac cgttaaatgt caggaacagc agtaacgcca
    10861 gcatatccat gatacgcgct aaaacgggca tattgagatg gctggccgga tcgacaaacg
    10921 tcgcaaatga cagccccatt tgcagaccga taatttcgcc agcggttcgc acagcggcaa
    10981 aggcaaattg catggtaaaa ccaagcgcaa tgccgatcag gatctgctgc acggccagcc
    11041 acagagcaaa gaacgaaaaa acaggaacat cgttggcagg taatgatggg gcaatgacga
    11101 acgtgatcat cattgccagc cccagtttta cccgtttcgg cacgctgcgt tcgctcagaa
    11161 ttggtgcggt agaaatgagc gccagcacgc gcagtaacgg ccagaagtac aggcttaacc
    11221 aggaaagcca ttgatcgctt gtcacctgca tcattgtacg gcctacccga tgatatacgg
    11281 caggttagtg aacaaggtgc ggacgtaatc cagcaacaga ttgagcatcc acggtccggc
    11341 aataataatg gcaataaata cggcgatgat ttttggaata aacgacagcg tcatttcgtt
    11401 gatctgcgtg gcggcctgca aaatactgat gataaggccc gtgaccagcg ccaccagcaa
    11461 cagcggggca gccagtgcca gcgcgacttt catcgcttca gtccccatca tcatgaccga
    11521 ttcaggtgtc attttgcctc tctagctgta aaagctctgc gccagcgaac cgaccagcaa
    11581 ttgccagcca tccaccagta caaacagcat cagtttaaag ggcagagcaa tggtggcggg
    11641 gggaccatca tcatccccaa tgccatcaac acgctggcaa tcaccaggtc gataatcaaa
    11701 aaaggaatga aaatcgtgaa gcctatctga aatgcggttt tcaactcgct ggtcacgtag
    11761 gccgggcgca aaatgcgcat cggcacggct tcaggtccct gcaacgggcc ggtattcgct
    11821 agtctggcaa acaaccctaa atctgcctca cgggtctgac gcagcataaa ctcacgtagc
    11881 ggctgcgccc ctttttccag cgcctcctgc attgatattt tctcttcgct gaatggctgg
    11941 tacgcatcga cataaatttt gtcgatcacc ggtgacataa taaaaaaggt caaaaatagt
    12001 gccagcccca gcaatacctg gttaggtggc gcggagggcg ttcccagcgc gttacgcaat
    12061 aaaccaaaaa caatgatgat gcgggtgaaa ctggtcatca tcagtaaaat tgccggaata
    12121 aacgtcaacg aggtgatgaa caccagcgtc tgcaccggga gcgaccagct ttgtccaccg
    12181 ccaggcagcg gctggctggt gatacccggc agttgcgcga aggcgagggg cgtaaccagc
    12241 cagagaagga caggtgcgac agacaacaaa cgacgcatca ggatctccca ctacgcttaa
    12301 gcaaattttt catgaccgac tgaaaatcgg tctgcggtat ctcttctgtt ggtgctgaag
    12361 ggggaagttt atgcagcaga ttgatttgcc ctgcggtaac gccgagcacc agccgtgcat
    12421 cttccacatc gaccaccaca acccgttcac gcgcgcccag cgaagcactg gcgctaattt
    12481 tcagaccgtt aacgccagtg cgtttcgggg caaatcccaa ccgttttacc tgccaggcag
    12541 cagcgaggat cagggcaata atggcgatga gtgcgccgct gacctgcagc agtggcgcag
    12601 cagaaaccgg cgtggaagat tgcacagtag cgtggttatt catcactaac ggctcaggcg
    12661 atgcattcgc tcagacggag taatgatatc ggtgatccgc acgccatatt tatcggcaac
    12721 gaccaccact tcgccctggg cgattaaata accgttgatc agaatatcca gtggttcgcc
    12781 cgccagaccg tccagcgcca cgacggaccc ttgcgtcaga cgcaacagct ctttgatggt
    12841 catccgcgta cggcccagct cgacggtcag cttgaccgga atatccatta tcaggtcgat
    12901 atcctgcaac gttccgctga catcaccacc gccaaattgc tggaacaccg cgtcggcagc
    12961 gcttttgctg ctggttgatt tttgttcgct caacgcttca gcccacagat cgtccattgc
    13021 gccgttgttg tcatcggccg gattattcat gtcactcatt tgggctgttc ctcgttcaga
    13081 gaatttaaaa tcgggttaat caaatgttct atccgtaacg catactgacc gttgagagtg
    13141 ccgtactgac tggtcagcac cgggacgccg tcaacatggg cgatgatgcg atcaggtttt
    13201 tctatcggca ggacgtcgcc gggtttcagt tttaaaatct gcgacaggcg tagcgagata
    13261 tcggcaaagt tggcgaccag ctccagctgt gaatgctgca cctggcgcac caggttatcg
    13321 cgccagttct gatcttcatt acgcgagttt tccagcggcg ggttaaccaa caattcccgt
    13381 agcggctcga tcatgctgaa tggcagacag atattaaatt cgccggtcag gttgccaatc
    13441 tccacatgga acggcgtgtt aaccacaatg tcgttcggcg aggtggtgat attggtaaat
    13501 ttcacctgca tttccgaacg cacgtactca acttccagcg gattaatcgc cttccaggcg
    13561 tcgctatagc cttcaagcgc cagctttaac atgcggttga tgacgcgctg ttcggtatgg
    13621 gtaaactcgc gaccttccac tttggtcggg aagcgtccat cgccgccaaa caggttatcc
    13681 acggcgataa acaccagact cggtgaaaac accaccagcc ctgtgccgcg cagcggtttc
    13741 agatggataa ggttcaggtt ggtcggcacc ggcaggttgc gggcaaattc atggtacggc
    13801 tgaatgcgga tggccccgac ggttatatcc gggctacgac gcagcaggtt gaacagcccc
    13861 atacgaaaat ggcgggcaaa gcgctcatta atgatttcca gcgcctgcaa acgttcgcgc
    13921 acaacccgtc gttgggtatt cggatcgtac ggacgaatgt cactttcgcc gctaacactg
    13981 gctgtcggtt cgtctttgac ttcgctgtca ccattcaaca gcgcatcaat ttcagcttga
    14041 gaaagaatac tatcgcccat gtcgttatcg cagaataaaa gcggtataca gcacgtcggt
    14101 gacatcctgt ttcggttgcc cggcaacaag cggggtggaa agcgtggttt taatctcggc
    14161 aatcaggttt ttcttgcctt cttctgtcgc cagtacggca gcatcctgac gcgaaaacag
    14221 taacagcaaa cggctacgga cttccggcaa atactcactc agccgcgagc gggtagcttc
    14281 atctttcagg cgcagggtta tgccgatata aagcacgcga tccgcatcgc ccaaattgac
    14341 cgtgaaggta ttcagcgcgt agaagaccgg ggagggtacg acgcgttgct gcgctttgtc
    14401 gtcagcggca acctgatgcg aatgccagta gctgtaacct gcgctggcac aggccgcgag
    14461 ggtaatgaat accagaatcg ggatccaaag cgatcgcttg cttttcttgc ttatcgcgta
    14521 atcagtcatg tgttgcggtc ttcctgtgtc gctactgctt atcggctaat tatcctgcgt
    14581 tttgagcgca acaaagcggg gaaaagacgc ggattacggt gctacctctg acgttaggcg
    14641 aaaatatcaa cgccgctgtt gcctgttaca cgcccttgta aagagacggg aaccggaagc
    14701 gtatcgtcgt cttcccccgc cagaggttca tggtttactg tgcgttggct ttgctgttgt
    14761 tgggaagcgg cctgctgctg accactaaag ctttcgccac tgatgttgct ttgccctaac
    14821 tgaatgccac tttcggccag ctgcgtacgc agtaccggaa gcgctgcttc cagcgcggcg
    14881 cgtacatgct gatgcggtga aaccatctgg atttgcgcct ggttatcatc cactttgagg
    14941 gagatttgca cttcacctaa atcctgcggg tgcagacgca actctgcact ttgttgcccc
    15001 tggcgggtga acagcgaaat atgctggctt aatgattgtt gccattcgtg agaacccagc
    15061 ggtgcactca aaacaggcgc ggcgacggtg ggcagtggct gtgtctggtg tggagtaatt
    15121 agcgggctgg cggcagcggt caccggtgac ggtgtgctga tgacttccgc tttactctgg
    15181 gcttctgcta ccagcggtgt taatggctga gctggcgtgc cgggggcatc atcaggctgt
    15241 gctgttgtga gttgcgcaga agtcagtttt gtgaagagcg ttggtttctc tgccggtaac
    15301 acggttgacg gcacatcagt cactttgggc gtattgtcaa aacccggcaa catcgcaaaa
    15361 agggcgctca ggcttgcggt gacgtcttca ttcagatcat ccgctttttc gtcttttgtc
    15421 gtattttggt cagccaccgc agccaacgtc atcgtctgag cggtggttaa cggtgttgat
    15481 gtggattgtt cgtcgttgat gacaggcagt gtttcatcca cggggatcag taaatccgct
    15541 tgttgcgcgt cggcaagaat atcgctgatc agcggctcgc cttttgtcgt gggcttatct
    15601 gttgccacca gcaactgggg ggccgctttg tcggtagttg tctcgcctgc taatgcttcg
    15661 ctcaacaacg cgagaaaatc ttgtgcagca tcgctggctt tgccgccagg caatgtggtg
    15721 gtgtcaacgt cggcggtaat taagggcgct aagcgaatca ttcaggtttc ctcatggcgg
    15781 cgcgctgggc gaactcatcc atctttttct gatcgaggcg gttttctgca agcagtgccg
    15841 ccgtggatag ccgttcctgc agtgtctgcc aggcctgcaa acgttgtttc ttttctcgcc
    15901 agctgttcag ggcaatgtca actttctgcg tccactgatt aagttgctgg cgatgctgag
    15961 taatggcttt ttccagcgtc tggataaact gctgatagtt gatccagcgg ttgctggtca
    16021 tcccggcact catatcgctg ttgaggttat tgcgatattc attctgataa tcgatcagca
    16081 ttttgagctg ttcttccgcc tgctgacacc cgcgacgcat ttcacccagc aggcgcgcgg
    16141 catcctctac ctctttttct gccagatctt tcagggtcgc cagcgcgcca tgttctgcca
    16201 tctgccgtta tctcctgggt tatgacactg tcgggaaaat acgctccagc ccctggagag
    16261 acgcttccca gtccgcgcgt tcaaaaatgc cttgttgcaa atagccctcc agctgcggcc
    16321 acagggcgat ggctttgtcg agcatcggat cgctgccttt ggaatacgcg ccgacgctaa
    16381 ccagatcgcg gttacgctga aaactcgaca acagctgttt gaaggtgcgc actcgcgcgt
    16441 aatgttgctc actgatcaac gccgtcattg cgcggctgat cgacgcttca atatcgatag
    16501 ccggatagtg cccggcttcc gccagtcggc gagacagcac aatgtgaccg tcgaggattg
    16561 cccgcgcaga gtcggcaatg gggtcctgct ggtcatcgcc ttcagtgagc acggtataaa
    16621 acgcggtaat cgatccgccg ccgctaatgc catttccggc acgctcgacc agtgccggta
    16681 atttggcaaa caccgacggg ggataacctt tagtggcggg tggttcgcca atcgccagcg
    16741 caatttcacg ctgggccatc gcgtagcggg tgagggagtc cataatcagc aacacatgct
    16801 gaccacgatc gcgaaaatct tcggcaatgc gcgtggcata ggcggcaccc tgcattcgca
    16861 ggagaggaga aacatccgcc ggagcggcaa tcaccacgga gcgtgcacgc ccttcggcac
    16921 cgaggatatt ctcaataaaa tcttttactt cgcgtccgcg ttcaccaatc aaacccacga
    16981 caatgacatc ggcgcgggtg taacgtgcca tcatccccag cagcacactt ttaccaacgc
    17041 cggacccggc aaacagcccc atacgctgcc cacgcccaac ggtaagcagg gcattgattg
    17101 ggcgcacgcc ggtgtccagc acatgttcaa tcggtgtacg ttgcaacggg ttaaatggcg
    17161 gggtaatcag cgcaccggtt tccgtcgtat cgggggaggc catcgagcgg tttaccgctg
    17221 ccgtccagaa cacgacctaa taacgccgga ccgagcggta actgtttgcc gctttgcagc
    17281 ccttctgccg aaatgttttt ggcataaaca cgcgcgccgg gcaggacacc ttcgacttcc
    17341 tccagcggca ttaaaaacag ccgttgaccg ttaaagccaa cgacttcact ttctacttcg
    17401 tgcgtttcag tgccgttctg gcgctcaatc acacaggttg cgccgagcgg caattgtaat
    17461 ccggtggctt ccagcaccag cccggtagcg cgggttaatc gcccgtagcg acgtaccgca
    17521 ggcaactgcg ccattttggc ttcaaagtta tccagcgtgg ttagccagcg agtcaggcgc
    17581 gtggtcatta caccactcct ggtgctgcca gacggcagag ttcttgccag cgagtggcga
    17641 cactggcgtc gagatcgcct tcatcggcgg agactttaca gccgccagga tggagggtgg
    17701 gatcgccccg caagcgccag ccatgcaaac ttaaggtagc gccgagcata tcatccacac
    17761 gttgcagatc atccgggtgc acgcgcagct gtggtttacc gctgaataac ggttcttgct
    17821 gcaacaactg ttggatctgt ttgatgagtg ccgagttatc cactgttggc gtctgaccga
    17881 tgacctgacg tgctgcctcc agcgccatct gcatcaggcg cgaagc
//
LOCUS       DADLFA010000074        17868 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000074 DADLFA010000000
VERSION     DADLFA010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17868
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.074"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            1..679
                     /gene="csgG"
                     /locus_tag="JQH65_002136"
                     /pseudo
     CDS             1..679
                     /gene="csgG"
                     /locus_tag="JQH65_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(743..1234)
                     /locus_tag="JQH65_002137"
     CDS             complement(743..1234)
                     /locus_tag="JQH65_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HAY6139540.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(1336..1890)
                     /locus_tag="JQH65_002138"
     CDS             complement(1336..1890)
                     /locus_tag="JQH65_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY6139541.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWMEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(1914..2651)
                     /locus_tag="JQH65_002139"
     CDS             complement(1914..2651)
                     /locus_tag="JQH65_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HAY6139542.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(2706..3644)
                     /gene="ghrA"
                     /locus_tag="JQH65_002140"
     CDS             complement(2706..3644)
                     /gene="ghrA"
                     /locus_tag="JQH65_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HAY6139543.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHWEDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            3878..3965
                     /locus_tag="JQH65_002141"
     tRNA            3878..3965
                     /locus_tag="JQH65_002141"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3912..3914,aa:Ser,seq:gga)
     gene            4010..4146
                     /locus_tag="JQH65_002142"
     ncRNA           4010..4146
                     /ncRNA_class="other"
                     /locus_tag="JQH65_002142"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(4237..4692)
                     /locus_tag="JQH65_002143"
     CDS             complement(4237..4692)
                     /locus_tag="JQH65_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HAY6139544.1"
                     /translation="MVAEGILLERKEWLITRCEKMYFDNLEKWFGHYCHIHCQVSLGR
                     LINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKIVCVIELDDPSHERADRIIR
                     DRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPLVAATKSEPVH"
     gene            complement(5504..6568)
                     /gene="phoH"
                     /locus_tag="JQH65_002144"
     CDS             complement(5504..6568)
                     /gene="phoH"
                     /locus_tag="JQH65_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HAY6139545.1"
                     /translation="MVTSCTGHVLDNQRATTRGVFSSGSHLVTLHFQPHPFFSCVTDA
                     VNGARSRFSAFYPKANYGLQGSQPSDVRAHNRAANGACDEYKQLKVLSMGRQKAVIKA
                     RREAKRVLRRDSRSHKQREEESVTSLVQMSGVEAIGMARDSRDTSPILARNEAQLHYL
                     KAIESKQLIFATGEAGCGKTWISAAKAAEALIHKDVDRIIVTRPVLQADEDLGFLPGD
                     IAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIGKVEIAPFAYMRGRTFENAVVILDE
                     AQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLPRGVCSGLSDALERFEEDEMVGIVR
                     FGKEDCVRSALCQRTLHAYS"
     gene            complement(6914..8185)
                     /gene="efeB"
                     /locus_tag="JQH65_002145"
     CDS             complement(6914..8185)
                     /gene="efeB"
                     /locus_tag="JQH65_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HAY6139546.1"
                     /translation="MQYKDENGVNEPSRRRLLKVIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFSLPGVKDANDYLGSALLRV"
     gene            complement(8191..9318)
                     /locus_tag="JQH65_002146"
     CDS             complement(8191..9318)
                     /locus_tag="JQH65_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HAY6139547.1"
                     /translation="MTINFCRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITAQAEDLAQLRGVLGLD"
     gene            complement(9376..10204)
                     /locus_tag="JQH65_002147"
                     /pseudo
     CDS             complement(9376..10204)
                     /locus_tag="JQH65_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
     gene            10240..10542
                     /locus_tag="JQH65_002148"
     CDS             10240..10542
                     /locus_tag="JQH65_002148"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6139548.1"
                     /translation="MQRKVNRSDSDYHYGKEKYKRNVYKMMLECKQREVMPGDINDYS
                     RVERWDCLQRTMCSCRMPRKRHIQPTKLSRLNASELPCKTDKRVYQAISSIRDYQI"
     gene            complement(10873..12381)
                     /gene="putP"
                     /locus_tag="JQH65_002149"
     CDS             complement(10873..12381)
                     /gene="putP"
                     /locus_tag="JQH65_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HAY6139549.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            complement(12540..12749)
                     /locus_tag="JQH65_002150"
     CDS             complement(12540..12749)
                     /locus_tag="JQH65_002150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6139550.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            12804..16766
                     /gene="putA"
                     /locus_tag="JQH65_002151"
     CDS             12804..16766
                     /gene="putA"
                     /locus_tag="JQH65_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HAY6139551.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNCNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLENLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLVDLATRSRRRLMIRQVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTYETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQCNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELHKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGIQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(16806..17444)
                     /gene="rutR"
                     /locus_tag="JQH65_002152"
     CDS             complement(16806..17444)
                     /gene="rutR"
                     /locus_tag="JQH65_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HAY6139552.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFIIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            17732..>17868
                     /locus_tag="JQH65_002153"
     CDS             17732..>17868
                     /locus_tag="JQH65_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HAY6139553.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     AL"
BASE COUNT         4489 a         4819 c         4380 g         4180 t
ORIGIN      
        1 ggtatacaac attcaggacg aaaccgggca atttaaaccc tacccggcaa gtaacttctc
       61 cactgctgtt ccgcaaagcg ccacggcaat gctggtcacg gcattgaaag attctcgctg
      121 gtttataccg ctggagcgct agggcttaca aaacctgctt aacgagcgca agattattcg
      181 tgcggcacaa gaaaacggca cggttgccat taataaccga atcccgctgc aatctttaac
      241 ggcggcaaat attatggttg aaggttcgat tatcggttat gaaagcaacg ttaaatcggg
      301 cggtgttggg gcaagatatt ttgggatcgg tgcagacacg caataccagc tcgatcagat
      361 tgccgtgaac ctgcgtgtcg tcaatgtgag taccggtgag atcctctcct cggtgaacac
      421 cagtaagacg atactttcct atgaagttca ggccggggtt ttccgcttta ttgactacca
      481 gcgcttgctg gaaggggaag tgggttacac ctcaaacgaa cccgttatgc tgtgtctgat
      541 gtcggctatc gaaacagggg tcattttcct gattaatgat ggtatcgacc gtggtctgtg
      601 ggatttgcaa aataaagcag aacggcagaa tgacattctg gtgaaatacc gccatatgtc
      661 ggttccaccg gaatcctgac agataaaaaa gccggaatta tcatccggca ttttactgtt
      721 taagcgaaag cgtgagtgcc cctcacgctt tatttttgat ttcctgctca cggtgagccg
      781 tctttgcact acgcgccgcc agccacaggc cgctgttttt cattgcgtaa ccaaatacca
      841 gcccaagcgc cagcgaaggt aacaccagtt tccagtcacc ctgcccggca aacgtcgcgc
      901 atgcgcctat aaacgtcccc ggaacaaatg aaagcagcag ctgtttggcc tgaatacaca
      961 tcagaaacgc cacaataccg gttatgacat aaccgagaat ttccagatgt ggtgccagtg
     1021 cactaccgta aataatgacc atcgcccaca ccacgccact aagcagggtt gcagcggaga
     1081 tcgccagccc tttcagccca ccttgcgggc aggcaaagta ggccgtacag cccaggaagc
     1141 ccgcccagct tagtaagccc agggaaacag ccacccatcc ccagataccg gagagaatgc
     1201 ctgttgtgat tgcaatagag agaagtatgt tcatgccgcg catgatagca gaaaacgcgg
     1261 ccatgaatga gatcgagcac acattttatg caacaacaaa ttatctgttg cagttatgag
     1321 tgacgatgat cacatttact cttcagaatc ttcttccagc tcgtcccaca ttgcactaat
     1381 ggcatcacgg gttagcggtg ccatggtgcg ccagaaaggc gtggttgcat gggcctccac
     1441 tttgccgagg aacgcaccac accagggtaa cagatactca ctgaacagtg tttccagtgc
     1501 ttcgctctca tcttccgttg actgatcttc catccaggaa gccgcaagca gcaatgtgcc
     1561 gatgtgatcg gctggcgtat ccgccagtgg catccctcgt tcggaaagaa aagcgcgcac
     1621 ttccgcttcc gttgcgtcct caacccatgc gctacgatat ggcggcacgg cacattcatc
     1681 gccgataaac aacgcattgt aatcggcaga gacttgcgcc atatcacagc ttttctgtaa
     1741 acgcgtcagt aactcatcct gctccagtgg ccagttcgca gccagtttcc cctcacgaat
     1801 cagggtaaac agcggcacca gtaaaggatc ttgcggttgg cggtaataga gcgaacccag
     1861 cacacgacag aggatagaaa actcgttcat taatatattt ccattactag taattaaagg
     1921 tctgcaaatt ccgcaatcgg tgccataccg cgagattcaa ggaagttcag taagcggcgc
     1981 ggagaaacat tcaaaatgcg ctctggcgga aaatctaccg cgtcgaggat tttaagacac
     2041 tcttcaaatt cccccatggt aaacgcggtg tgagaatccg agcctaatgc cacccaacct
     2101 ccagcatcac gtaccgctgc ggctaccgca cgacagttgt cttcactgcc cttacgtgag
     2161 tgtaaaaatg aagaattatt gatttccagc gccacctgat gtttcgcggc tgcttcagca
     2221 acggctttca catctatttc atatttggga tttccgggat ggcttattat atgcacattg
     2281 ccgctggcga tagtggcgat catcgcttgt gtattggtcg ctttgtcatg tggtgcaaaa
     2341 accggctcat gaaaaccggc aataatcaga tccagcgagt caaacatttt accgctgcaa
     2401 tcaatttcac catcaacatt tttaatgtta gcttcgatgc cgcgcaggat ccctacccca
     2461 tcaaccactc gcggccagat acgcatgtta atgaagtgcc agtgatgcgg agcatcttcc
     2521 atatccgggc catgatcggt gatcgcaaaa agtttaatgc ccttttgttt ggcctgggca
     2581 atgtaatcac tcaatgtgct atatgcatgt gtgctggcaa cggtatgcat atgaaggtcg
     2641 acgggataca taactctctc ctgtattcaa ttcccgccaa ggatagcagg aatcctgatg
     2701 ctttattagt agccgcgtgc gcggtcgact tgcccgcaga ccctctcccc tttttcgagc
     2761 tgggcaatgg tgcgagaaat gtactccaca gcttcagcgg gacgggtaat cgcggcgaca
     2821 tgtggtgtta tcgtcacgcg tggatgttgc cagagcggac tttcaggcgg taagggttca
     2881 cgattaaaaa catccaacat tgcgccttta actttgccgc tatccagcgc cgcgagcagg
     2941 tcatcttcca caacatgaac accacgcgcc aggttgagga gatacgcgcc atccggtaat
     3001 ttttcgagta attgttgatt aataatgccg acggtttcag gggtattcgg taacaaatta
     3061 atcaataccc gacattggct cagaaatgca gacagttctt cccgtccggc aaagctttgc
     3121 acgccaggcc acgatttacg ggttcgactc cagcaacgca gcggaaagcg ccaggtttgc
     3181 agactctgag caactttact gcccagtacg cctgcgccca aaatgccgat ggtaaaatct
     3241 tcccaatgat attcaggcag cggttgccaa tgcgaactat tttgctggat gcgataatcg
     3301 tcaaaacgtc gaaaccaatg cagcacctga ctgacagcat attcctgcat ttgctcgccc
     3361 ataccagtat cttccaggcg aaaaagtgga acagaagggt tcagcatttc agggtgtgcc
     3421 tgtagcttgc tcaaaataga atcaacaccg gccccgagtg cgaacaccgc tttaagatcg
     3481 cgccctgcca gcatttcaac aggaggatgc cagactaaag cataatcagc agagtcatta
     3541 tctccgcttt tccatgctct gactcttgcc tgaggaatag ctttgcgcag tgcctcaatc
     3601 caccattggg tatcgaacgt tgggtgataa aagatgatat ccatactgac tcccgaaaag
     3661 cgttgtgcga aatttattcg cacttatcgt tatgatctac aaaggccacc agcataacaa
     3721 atccgtggtc ggtggcaaaa aaagcagatt tcgcttatta aaaccacaca ttgattgaag
     3781 tttgaataaa cgcgcgattt tttcaaaaag tttgttgacc tcaggtcatg atttccctaa
     3841 attagcgccc gttccagcaa gacaggaaca acaatttggt gaggtgtccg agcggttgaa
     3901 ggagcacgcc tggaaagtgt gtatacggca acgtatcggg ggttcgaatc cccccctcac
     3961 cgccatattt aaagaagagc tcgtacgaaa gtacgggctt ttttttcgta tattgcacac
     4021 accgggggga tgagaagccc cgaccggggt tcgacaactg gcgacagcca gttggacaga
     4081 ccgtgaacgc agtgagcggg ctgcccgcag ggcgagcgaa gcgagtcaat ccccccctca
     4141 ccgccatctt taaagaagag ctcgtacgaa ggtacgggct ttttttcgta tattgcacac
     4201 accgggggga tgagaagccc cgaccgggat acttagttaa tgcaccggtt cactctttgt
     4261 cgccgcgact aatgggcgaa ttttgtcacg agtctcccag accggaggaa tttcctccaa
     4321 tttagtgaca gaagctcgta caaaaggcac ccgtgcaaga gtcataattg tatccaacca
     4381 acggtcacga ataatgcgat ccgccctttc atgactcggg tcatccagct caataacaca
     4441 gactatttta ttgttcttct ttgaaaccag aacataatcc attgccatgc cattataaat
     4501 tgcaaagaaa cgctttcttt cttcttcgct aaactcaggt tgttctggaa agttgatcaa
     4561 gcgccccaga gagacctggc aatggatatg acaataatgt ccaaaccatt tttccagatt
     4621 atcgaaatac attttttcac aacgagtgat taaccactct tttctttcca ataaaatacc
     4681 ttcggcaacc attccctgaa ataactcatc tcgctcttgc caacgagagt cacgataggt
     4741 tttctgcggt atatagctcc ttgtcgacgc cttagcggca ggggaagttg cagatgaagg
     4801 gacgggttgc gatttagcga tgattaccga tgaggcaact tttcggagtg tcaattttct
     4861 ggcaacagca aaaccggcga taaatgcaat tgtcaaacaa gttacaaaag ccataaagct
     4921 cataattaat tttcacctat ccatgttgat tatatttcat taccaatgtt actcgctgca
     4981 gcgtgttaca tgccaacaat gcaacgatca atatatagtt agtatcatgt tttgattatt
     5041 acaaccatcc ctatttagcc attgcggctt gccttccaca aaaaaataac aacacacaat
     5101 attagcaaag aattaattaa gattaacctt acatttataa tattatccct aaagaataaa
     5161 tcagaggaat atattcggtg ttaaacagtc agtcaaagta tagcccattg ttgattaaac
     5221 ctcataagca aaattggcac cagatctcct gtacaggttg aatggccgaa aaaagatagg
     5281 cagatgctca tttttttggc ttctggaaga aaaactctcc gcgctggcag tattacactg
     5341 gtctgatgct atgacgaaaa atgcctggta agagtatcta aaccagactc cagttcagct
     5401 tatcaacacg ctcaaagtcg cccccctgaa cgctaacctg aagtgaaaat ctgttgaaat
     5461 aaaacaaagc ccggttcgcc cgggctctgc accgataaca cacttaactg tatgcatgca
     5521 gcgtacgttg gcaaagtgcc gaacgtacgc aatcctcttt tccgaaccgg acgatcccaa
     5581 ccatttcatc ttcttcgaaa cgttccagcg cgtcacttaa tccggagcac acgccgcgag
     5641 gcaaatcgca ttgcgtgata tcaccgttga cgataaccgt cacgttctcc ccgaggcggg
     5701 ttaaaaacat tttcatttgc gcggcagtca cattctgcgc ctcgtcaaga atgacgactg
     5761 cattttcaaa ggtacgtcca cgcatatagg cgaacggcgc gatttccacc ttccctattt
     5821 ccggtcgcag gcagtactgc ataaaggaag cccctaagcg ccggaccagc acgtcgtaga
     5881 ccgggcgaaa atagggagca aacttttctg cgatatcacc aggtaagaag ccaagatctt
     5941 catcggcttg cagaactgga cgggtgacga taatcctgtc gacatcctta tgtatcaggg
     6001 cctctgccgc ttttgctgcg ctgatccagg tttttccgca cccggcttcg cccgtggcga
     6061 atatcagctg cttactctca atagccttca gatagtgcaa ttgcgcttca tttcgcgcga
     6121 ggatgggcga ggtatcgcga ctgtcgcggg ccataccaat ggcttctacg ccgctcatct
     6181 gcacaagcga ggtgaccgat tcttcttcac gctgcttatg gctgcgcgaa tcccgtctca
     6241 gcacacgttt tgcctcgcga cgagctttga tcactgcttt ttgtcttccc atggagagca
     6301 ccttgagttg tttgtattca tcacacgcgc cgttggcagc gcgattatgc gcacgaacat
     6361 cagagggttg gcttccttgt aagccatagt ttgcttttgg ataaaatgcc gaaaaacggc
     6421 tacgcgcacc gtttacggcg tcggtaacac atgaaaagaa aggatgaggt tgaaaatgca
     6481 aagtgacgag atgactaccg gaggagaaaa ctccgcgagt ggtggcgcgt tgattatcta
     6541 aaacatgtcc agtacaggac gttaccatcc gcgatctcca tagtgactga ctatcactgc
     6601 cgggaacttc cgctgctact taataagtac aacagatctc gcattttatt gcaacaatat
     6661 atttacttat atttaactat aaaacactat ttcagtgaca ttagtttcta ctggaaagat
     6721 gacagagtga tgacagtgat gaaaaaagct gtgtgctttc agcaggattt gcatcaggca
     6781 tcagtgcacg atcgccggat gcggcgtgaa cgccttatcc agcctaccaa acccgcactg
     6841 attgtaggca tgataagacg cgcaagcgtc gcatcaggca tctgcgcaca aatgccttat
     6901 ccgcctaaaa acattaaacc cgcaataacg cgcttccgag ataatcgttc gcgtccttca
     6961 ctcccggcag cgaaaaaaaa taaccgccgc cgataggttt aacgtattcc tccagcgctt
     7021 cgccattgag ccttttttgt actgtcagga agcctttttc cagatcgtgt tggtagcaga
     7081 caaacagcaa ccccatatcc agttgtccgg agttggtgac ccccaacgaa taactgtagc
     7141 cgcgacgcag catcaggctg gactcactct ccgccgtgcg gggattcgcc agccggatat
     7201 ggctgtccag cgcgatcacc ttcccttccg ggtcgctggc gtaatcaggc acatcatgct
     7261 catgctgcat tcccagcggc gcaccggttt gcttatcacg gccaaaaatc gtctgctgtt
     7321 ctttcagcgg cgttctgtcc caaaattcca ctcgaaactg aatcaagcgt actgcctgat
     7381 agctgccacc gattgtccac gcaggctcct gctgatctgc cgttacccac accacttttt
     7441 gcatcaactt atcattctgg ctatcgggat tggcagtgcc gtctttaaag cccagcaaat
     7501 taatcggcgt ctctttgcct ttactacgcg ctgcgtgatc ggaaataaac ccttcccgct
     7561 tccagcgcac gctgagcaaa tccggcgtgt gtttgatgat atcgcgcagc gcatggatca
     7621 ccgtgtcctg ggtgttggcg caaatttgta gcaacacatc accatggcat aacgccgcat
     7681 ccagcgagtc gttggggaaa cgcgtcatct tctgcaactt tttcggcatc tgtggcgcaa
     7741 ggccaaaccg ctcatcaaac aatgagtgac ccaccgataa cgtgatggtg agattatcgg
     7801 gcgcaatgta gccgccaaga atgccggaat cgagtggtgg caggcgcgga tttggcgttt
     7861 ctggtgccgc tccgccctga gtcagaaaag caaaacgctg agtcaacaag cgaaacaacc
     7921 gttcaagatc ggctttatcg ctggcaagca catcaaacgc caccagcatc attgcggcct
     7981 gttgtggcgt caggatccct gcctgatgct caccataaaa cggctgtttc tcattgcgtg
     8041 catccggtga aagcgtaccc ggcgcacttt gcgttttttg tgcatgagcg accggacaac
     8101 ttcccgccag cgccagcgca cctatcactt tcagtaaacg tcggcgtgac ggttcattca
     8161 cgccgttttc atctttatac tgcataacgc ttaatccagt cccagcacac cgcgaagttg
     8221 cgccagatct tccgcctgcg cagtaatcgg tcctttcagt gcattccggt cggcatcggt
     8281 caatttgtcg taggtttcaa aaccgtcttt agtacggtat ttcgccagaa tggtatcgac
     8341 ctttttaaag ttggcatcga cttttgccag cagttccggg ttggcttttt gcagttgtgg
     8401 acgcagcaaa tcgacaattt tctgcgagcc ttcaacgtta gcctggaaat cccacagatc
     8461 ggtgtggctg tagcgatctt cttcaccgct aattttgctg gctgccactt cctcaatcag
     8521 tccggctgcg ccgccgacca cttttgaagg tgggaaagcc agttcactga tgcgtttttg
     8581 caaatcgacc acatctgtat aaagctgctc agcgtactga tccatccctt tggtggtgtt
     8641 gtcgccaaac aatgcttttt ccagacggtg gaaaccagtg aattttgggt cggcggcttt
     8701 ttgctcgtaa tcatcttcac gggcgtcaat gctgccatcc agatcggaga acagttcagc
     8761 aatcggttca atgcgctcat agtgctggcg cgtcggcgca tacagtgctt tcgctttttc
     8821 gatatcgcca gctttaatcg cgtcggtaaa ggctttggtg tcggtcacca gctgcgtggt
     8881 ttccgccatg acatacgctt tatatgcagt aattgcacca ccaagactta acagcgcatc
     8941 actttgcgcc gcatccgccg ttgcctcacc tttgacgatc aacttccctt tcgggttagt
     9001 cagcagaccg caggtcatat cgtattcgcc aggctgtaaa ttcgccgtca ttttctggct
     9061 aaaaccaggg gcgatatttt cccgctcttc caccaccatc acgcctttga ggatctccca
     9121 ctccagcgcc ttctggctat ggttctgaat aatgaactgt gttttcccgg cgttaaccgt
     9181 aatggtcatc ggttcgcact gcttatccgt cacggtcact ttgacctgcg gcacatcagc
     9241 ggcgttagcc ataaaagcag aagaaaacag cgcagccacg ctcaactgca atgcgttacg
     9301 gcagaagtta atggtcatga cgagactatc cctttaaagt atgttgtaac taagcaagag
     9361 tttgcgtcgt atttgttacg cggagcgaga cgctgtcgcc cctgcgcgtg gtggcagagc
     9421 aaatgccacc agcgccggga tgagataaat aaaccagacg gcgacttcgc tgacgctcgg
     9481 cgcttcctga tagccaaaaa tcccttccat cagcgtgcca aacagcgagt gagttgagag
     9541 caccgcactc atatcgaagg cgatttcctg aaagtggttc cacaatccgg cttcatgaaa
     9601 tgcgcgaatg gcaccagctg ccagccctgc ggcgacgaag agaataaaca ggctggtcca
     9661 tttaaaaaat gcaccaagat tgaggcgaat accgccccag tagagcagaa agccgagcac
     9721 cacggctgta gcaagaccga gcattgcacc cagcggcggc cagatcccga catcttgttg
     9781 aaatgccgcc agcaggaaaa agaccgactc cagcccttcc cttgcaacgg caaaaaagac
     9841 catcatcacc agcgcccagc catgatgatt tccacgctac aatgcgctat cgactgcctg
     9901 ttccagttgc acttacgttg cgcgacactt tgcgcatcca gaaaaccatc caggtaagga
     9961 tcaccacggc gatcaccgcc acgatacctt caaacagttc ctgttctttt tgcggaaatt
    10021 cgccggtggt ttcgttaatg aagatcccca ggcccaggca caacgcagcg gcaagcaaca
    10081 cgccaatcca catcacacca atccatcggc ctcgctgggt acgcttaaga tagctggcaa
    10141 tcaaactgac aatcagcgcg gcttcaagtc cttcgcgcaa cataatgaga aacggaacaa
    10201 acatgccacc cacccttaaa cgtttttctg agtcaatcta tgcaaagaaa agtaaatcgc
    10261 agtgatagcg attatcatta cggtaaagaa aaatacaagc ggaatgttta taaaatgatg
    10321 ttggaatgta aacaaagaga ggtaatgcca ggggatataa acgattattc cagggtagaa
    10381 agatgggatt gtttgcaaag aaccatgtgt tcttgccgga tgccgcgtaa acgccatatc
    10441 cagccgacaa aactgtccag actcaatgca tcagagcttc catgtaaaac tgataagcgg
    10501 gtgtatcaag caatttcgtc catccgcgat tatcaaatct aactttttgc ccggaacata
    10561 ataacagctc gggtgttggt ggcgtcaaaa tcgctgaagc gttccccgaa ggccgggaca
    10621 gcccacatgg atgtgggctg agggcgcgtc ttacaggacg ttacctcgcg cccgacccgg
    10681 tagccgaagg ggataagctg aaggtaccgc gcagcggcga ttttgttcgc cagagcccgg
    10741 gggtgcacag ggggcggcgg cgattggccg ccccctgcgc gctccctgca ccattcaatt
    10801 tatatggcga ggcacattaa gggagcgcaa cccgctgctg aagccaaaca aaaccgccgc
    10861 aggctaagtc ccttagcttt cctgcaaccg tgacggcgga gccgaatgat agtgcgcatc
    10921 ggcctcggca aagcgttttt gcatcgccgc tgacggcgct ttacccagca aactaaacac
    10981 tacaatcccg atactgccga agatgaagcc cggaataatt tcgtacagtc ccagccagcc
    11041 gaactgcttc cagacgataa ccgtcagcgc accgatgatc atccccgcca gtgcaccgtt
    11101 acgcgtcatg cgtgaccaca tcaccgagaa cagcaccacc ggaccaaacg ccgcgccaaa
    11161 gcctgcccac gcgtagctca ctaagcccag cacgcggttt tccgggtttg ccgccagcgc
    11221 aatcgccacc agcgccacca ccagcaccat cacacgccct acccacacca gctctttctg
    11281 gctggcatgt ttacgcagaa acgctttgta taaatcttcg gtaatcgcac tggagcacac
    11341 cagcagttgg caacttaagg ttgacattac cgccgccaga attgccgaca gcagaatccc
    11401 ggcaatccac gggttaaaca gaatttgcgc cagttcgata aacacgcgct cggcgttctg
    11461 gtttaccgcg cctgctaccg ccgggtgctc attaaagtaa gcaatcccaa agaagccgac
    11521 agccaccgcc cctgccaggc agaggatcat ccaggtcata ctgatacgac gcgcatggac
    11581 aatgctgtgg tgagaatccg ccgccataaa acgcgccaga atgtgcggct gcccgaagta
    11641 acccagcccc caacccatca gtgagataat ggcaacaaag ttcagacctt tgagcatatc
    11701 aacgttttcg atgctctttt gtttgatcac ttccagcgag tcaccaaagc caccgacact
    11761 gataatgacg ataaccggtg tcaggatcag ggcaaaaatc atcaggctgg cctgtacagt
    11821 gtcagtccag ctcaccgcga ggaaaccgcc aataaaggtg taaaggatcg tcgccgcagc
    11881 tcccgcccac agagccgttt cgtagctcat gccaaaggta ctttcaaaca gacgcgcgcc
    11941 tgccacaatg cccgaagcgc aataaatggt gaagaacagc aaaataacca gcgcggagat
    12001 aatgcgcaaa atgcggcttt tatcttcaaa acgcccggtg aaataatccg gcaatgttaa
    12061 ggcgttatta ttgtattcgg tatgcacacg caaccgcccg gccaccagct tccagttgat
    12121 ccacgcgcct aatgtcaggc caatggcgat ccagctttcg gaaatcccgg aaagaaaaac
    12181 cgcgccaggc aaccccatta acagccagcc gctcatatcc gacgcacccg ccgataatgc
    12241 cgtcacaaat ggcccaagac tacgaccgcc cagaatatag tcgtcaaagt ttttcgttga
    12301 tcgccaggcg ataaacccaa tcaatatcat gccaaagata tagacacaaa atgtcaccaa
    12361 catcggtgtg ctaatagcca tctaaagtct ccaaaaaatt attatcggca atgtcgaaac
    12421 ttgccgttat atctgcaacc ggaacggggt aacagagttt attttttacc agggcgaccg
    12481 tatcctgccg gaagcgctgg ttattcacaa tcgatttaac acaccattta cattaaattt
    12541 tagtgctcag cgacactatt tttcatcagg ttgcactctc tcacattttt tgcggttgca
    12601 cctttcaaaa atgttaactg ccgcagagaa aaagtctgag ttattttttt aatccctgtc
    12661 atatcaattt cttttattaa catttcattc atttttaagc ttgctacgca ggtcacattt
    12721 aacatggttg cacaaagttg caacatcatg gatatttcac gataacgtta agttgcacct
    12781 ttcagaacaa caggagtaat ggcatgggaa ccaccaccat gggggttaag ctggacgacg
    12841 cgacgcgtga gcgtattaag tctgccgcga cacgtatcga tcgcacgcca cactggttaa
    12901 ttaagcaggc gattttttct tatctcgaac aactggaaaa cagcgatact ctgccagagc
    12961 tacctgcgct gctttctggt gctgccaatg agagcgatga agcaccgact ccggcagagg
    13021 aaccacacca gccattcctc gactttgccg agcaaatttt gccccagtcg gtttcccgcg
    13081 ccgcgatcac cgcggcctat cgtcgcccgg aaaccgaagc ggtttctatg ctgctggaac
    13141 aagcccgcct gccgcagcca gttgctgaac aggcgcataa actggcatat caactggccg
    13201 ataaactgcg taatcaaaaa aatgccagtg gtcgcgcagg tatggtccaa gggttattgc
    13261 aggagttttc gctgtcatcg caggaaggcg tggcgctgat gtgtctggcg gaagcgttgt
    13321 tgcgtattcc cgacaaagcc acccgcgacg cgttaattcg cgacaaaatc agcaactgta
    13381 actggcagtc acacattggt cgtagcccgt cactgtttgt taatgccgct acctgggggc
    13441 tgttgtttac tggcaaactg gtttccaccc ataacgaagc cagcctctcc cgctcgctga
    13501 accgcattat cggtaaaagc ggtgaaccgc tgatccgcaa aggtgtggat atggcgatgc
    13561 gcctgatggg cgagcagttc gtcacgggtg aaaccatcgc ggaagcgtta gccaatgccc
    13621 gcaagctgga agagaaaggt ttccgttact cttacgatat gctgggcgaa gctgcactga
    13681 ctgccgcaga cgcacaggcg tatatggttt cctatcagca ggcgattcac gccattggta
    13741 aagcgtctaa cggtcgtggc atctatgaag gtccgggcat ttcaatcaag ctgtcggcgc
    13801 tgcatccgcg ctacagccgc gcccagtatg accgggtaat ggaagagctt tacccgcgtc
    13861 tgaaatcact caccctgctg gcgcgtcagt atgatattgg tattaacatc gatgccgaag
    13921 aggccgatcg tctggagatc tccctcgatc tgctggaaaa tctctgtttc gagccggaac
    13981 tggcaggctg gaacggcatc ggttttgtta ttcaggctta tcaaaaacgc tgcccgttgg
    14041 tgatcgatta cctggttgat ctcgccaccc gcagccgtcg ccgtctgatg attcgccagg
    14101 tgaaaggcgc gtactgggat agtgaaatca agcgtgcgca gatggacggc cttgaaggct
    14161 atccggttta tacccgcaag gtgtataccg acgtttctta tctcgcctgt gcgaaaaagc
    14221 tgctggcggt gccgaatcta atctacccgc agttcgcgac gcacaacgcc catacgctgg
    14281 cagcgattta tcaactggca ggtcagaact actatccggg tcagtacgag ttccagtgcc
    14341 tgcatggtat gggcgagcca ctgtacgagc aggtcacagg gaaagttgcc gacggtaaac
    14401 ttaaccgacc gtgtcgtatt tatgccccgg ttggcacata tgaaacgctg ctggcgtatc
    14461 tggtgcgtcg cctgctggaa aacggtgcta acacctcgtt tgttaaccgt attgccgaca
    14521 cctctttgcc actggatgaa ctggtcgccg atccggtcac tgctgtagaa aaactggcgc
    14581 aacaggaagg gcaaactgga ttaccgcatc cgaaaattcc cctgccgcgc gatctttacg
    14641 gtcacgggcg cgacaactcg gcagggctgg atctcgctaa cgaacaccgt ctggcctcgc
    14701 tctcctctgc cctgctcaat agtgcactgc aaaaatggca ggccttgcca atgctggaac
    14761 aaccggtagc ggcaggtgag atgtcgcccg ttattaaccc tgcggaaccg aaagatattg
    14821 tgggctttgt gcgtgaagcc acgccgcgtg aagtagaaca ggcgctggaa agtgcggtta
    14881 ataacgcgcc aatctggttt gccacgcctc cggctgaacg cgcggcgatt ttgcatcgcg
    14941 ctgccgtgct aatggaaagc cagatgcagc aactgattgg tattctggtg cgtgaagccg
    15001 gaaaaacctt cagtaacgct attgccgaag tgcgcgaagc ggtcgatttt ctccactact
    15061 acgccggaca ggtgcgggat gatttcgcta acgaaaccca ccgtccatta gggcctgtgg
    15121 tgtgtatcag tccgtggaac ttcccgctgg ctattttcac cgggcagatc gctgccgcac
    15181 tggcggcagg taacagcgtg ctggcaaaac cggcagaaca aacgccgctg attgccgcgc
    15241 aagggatcgc cattttgctg gaagcgggtg taccccctgg cgtggtgcaa ttgctgccag
    15301 gtcaggggga aaccgtgggc gcgcaactga cgggtgatga tcgcgtgcgc ggggtgatgt
    15361 ttaccggttc aaccgaagtc gctacgttac tgcagtgcaa tatcgccagc cgcctggacg
    15421 ctcagggtcg ccctattccg ctcatcgctg aaaccggcgg catgaacgcg atgattgtcg
    15481 attcttcagc actgaccgaa caagtcgtcg tggatgtgct ggcctcggcg ttcgacagtg
    15541 cgggtcagcg ttgttcggca ctgcgcgtgc tgtgcctgca agatgagatt gccgaccaca
    15601 cgttgaaaat gctgcgcggt gcaatggccg aatgccggat gggtaatccg ggtcgcctga
    15661 ccaccgatat cggtccggtg attgatagcg aagcgaaagc caatattgag cgccatattc
    15721 agaccatgcg tagcaaaggc cgtccggtgt tccaggcggt gcgggaaaac agcgaagatg
    15781 cccgtgaatg gcaaagcggc acctttgtcg ccccgacgct gatcgaactg gatgactttg
    15841 ccgaattgca caaagaggtc tttggtccgg tgctgcatgt ggtgcgttac aaccgtaacc
    15901 agctaccaga gctgatcgag cagattaacg cttccggcta tggtctgacg cttggtgtcc
    15961 atacgcgtat tgatgaaacc atcgcccagg tcactggctc ggcccatgtt ggtaacctgt
    16021 atgttaaccg taatatggtg ggcgcagtgg ttggtgtgca gccgttcggc ggcgaagggt
    16081 tgtccggtac cgggccgaaa gcaggcggtc cgctctatct ctaccgtctg ctggcgaatc
    16141 gcccggaaag tgcgctggca gtgacgctcg cgcgtcagga tgcagagtat ccggtcgatg
    16201 cacagttgaa agccgcattg actcagccgc taaatgcact gcgggaatgg gcagcaaatc
    16261 gtccagaatt gcaggcgtta tgtacgcaat atggcgagct ggcgcaggca ggaatacaac
    16321 gattgctgcc ggggccgacg ggtgaacgca acacctggac gctgctgccg cgtgagcgcg
    16381 tgttgtgtat tgccgatgat gagcaggatg cgctgactca gctcgccgcc gtgctggcgg
    16441 tgggcagcca ggtactgtgg ccggatgacg cgctgcatcg tcagctagta aaggcattgc
    16501 catcggcagt cagcgaacgt attcaactgg cgaaagcgga aaatataacc gctcaaccct
    16561 ttgatgcggt gatcttccac ggtgattcgg atcagcttcg cgcattgtgt gaagccgttg
    16621 ctgcgcggga tggagcaatt gtttcggtgc agggttttgc ccgtggcgaa agcaatatcc
    16681 ttctggaacg gctgtatatc gaacgttcgc tgagtgtgaa taccgctgcc gctggcggta
    16741 acgccagttt aatgacgatc ggttaaacag gtagccggag gatgttacac cctcctccgg
    16801 catctttaac gtggtcgaat cccctcaata ataatccgct gcacgttttc aaccgtttga
    16861 ttgaaaaata cctcatcgcg caacgttgcg cctgtcaccg cctccacctg aggggcgaaa
    16921 tcggcgtaat gttgagtgga agcccaaata ataaaaatca aatgctgcgg atcaatcggc
    16981 gcgagtttgc cgcttttgac ccaaccggca atcagcgccg atttctcatc aattaatgcc
    17041 ttcaaatcgc ccgtcagttc atccattaac agcggcgcgc ctgccagcat ctccatacag
    17101 aacaggcgcg aagcctgcgg ataatcgcgt gagacttcca gcttcagacg gatgtactct
    17161 ttgatcgccg ccagcggggc gaaatcttca cgaaacgctt ttaacggtgc cagccagata
    17221 tcgagaatct gccgcagcac ggcaatatac agcgcctctt ttgacggaaa gtaatacagc
    17281 agattggttt ttgaaacacc cgccaactct gcgatctgct ccagccttgt gccgtgaaaa
    17341 ccgaattgtg aaaaagtgtc cagtgctgcg ctaagaatcg cttttttctt cgcgcttact
    17401 gcgcgcgaac gtttacccgt tgttttcact gcgccttgcg tcatgcgctc tcccctcttg
    17461 tagattggca acaggatagc aaaacccccg cccggcctcg accttctgca ctctcatcgc
    17521 gcacggatgc atgttttatg tgcaactgtt ttgaccgttt agtccacttt ttaccagata
    17581 ttttagataa cacaatgcta acatattaat aacattcact ttttaaaaac tggcatccgc
    17641 tttgcaaaca agccaatacg cggctgaaaa ggatatgcag gatgcagcgc cccgtctgac
    17701 tttcacttta cgagacgaag agaggttgat gatgaaaatt ggcgtattcg tacctattgg
    17761 caacaacggc tggctcattt cgacccacgc gccgcagtac atgccgacct ttgaactgaa
    17821 taaagccatt gtgcaaaaag cggagcatta ccatttcgat ttcgccct
//
LOCUS       DADLFA010000075        17798 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000075 DADLFA010000000
VERSION     DADLFA010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17798
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.075"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..385
                     /locus_tag="JQH65_002154"
     CDS             <1..385
                     /locus_tag="JQH65_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY6139554.1"
                     /translation="LAEVRQHTWSSGTNGVVAATFSLRLKGKPVSFVVPLAFVKNLDK
                     TLTVNTGALLTMSVSANGGMPPYKYAWKKDGQPVDGQTTDTFSKPGAQSADAGKYTCV
                     VTDSAEKAQSVTSVECTVTVSAAAG"
     gene            400..774
                     /locus_tag="JQH65_002155"
     CDS             400..774
                     /locus_tag="JQH65_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY6139555.1"
                     /translation="MKKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            870..1079
                     /locus_tag="JQH65_002156"
     CDS             870..1079
                     /locus_tag="JQH65_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HAY6139556.1"
                     /translation="MWMEFDRISPLGDERGDIRNAQIVKAVFGAQGMNVALKDVMLCW
                     GEDEDKPEVDPFAALEDALSFAAQS"
     gene            1127..1387
                     /locus_tag="JQH65_002157"
     CDS             1127..1387
                     /locus_tag="JQH65_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6139557.1"
                     /translation="MATLRELIIKIVSFRGTHIDAFFRRASSAAHTNARGLITVDLFC
                     CTEAIARQVPEAGSEVLSSVIASGREVLTRGLIAGDDVLTRE"
     gene            complement(1522..3123)
                     /locus_tag="JQH65_002158"
     CDS             complement(1522..3123)
                     /locus_tag="JQH65_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc49 family transposase"
                     /protein_id="HAY6139558.1"
                     /translation="MNIRIWSGILPCMDISALNTTNDIEKLRAMALAMVQEVMSENAE
                     KERELLEKSRRIQLLEEMLKLVRQQRFGKKCETLAGMQRSLFEEDVDADIAALTAHLD
                     KLLPQSPEEDEKASRSRPIRKPLPVHLPRVEKMIQPDTDHCPECDEPLHYIRDAVSEK
                     LEYIPAHFVVNRYVRPQYSCPCCQKVFSGEMPAHILPKSAVEPSVIAQVIINKYGDHL
                     PLYRQQQVFARSDVGLPVSSMADMVGAAGAALSPLAALLHRELINRPVVHADETTLKI
                     LNTKKGGKSCSGYLWAYVSGERTGPSVVCFDCRTGRSHEYPENWLQGWGGTLVVDGHK
                     AYRTLANKVPEITLAGCWAHARRGFADLYKISKDPRAAIAVKKIAGLYRLEKKISSRP
                     VEKIRQWRQRYARPILEELWSWLEEQEPQCSPGKALHKAIAYALSHRVELSRFLEDGA
                     VPLDNNVCERAIKNVVLGRKSWLFAGSQMAGERAAQIMSLLETAKRNGLEPHAWLTDV
                     LMRLPEWPEERLAELLPLEGFTFSG"
     gene            complement(3130..3522)
                     /gene="tnpB"
                     /locus_tag="JQH65_002159"
     CDS             complement(3130..3522)
                     /gene="tnpB"
                     /locus_tag="JQH65_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAY6139559.1"
                     /translation="MLSPDNVFIAIKPVDMRRGIDSLTQYIQDELRSTWHEGAAFVFV
                     NKVRSRIKVLRWDKHGVWLCTRRLHKGSFRWPRANDAAWHLTPDEFNWLIAGVDWQQV
                     KGHDLTKWVWQNEPELRPENTKNTLLTQ"
     gene            complement(3509..3838)
                     /locus_tag="JQH65_002160"
     CDS             complement(3509..3838)
                     /locus_tag="JQH65_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HAY6139560.1"
                     /translation="MSKPRWTIEQKRQHVAAWRASGLTRQQYCELNDIPFTSLREWPQ
                     DVAKAERRANEPTVLPVHIAPPLHADVPQPVTNEPVTLFLPGGVRMCCQPSQLTDVFR
                     ALKYAQS"
     gene            3883..7143
                     /locus_tag="JQH65_002161"
     CDS             3883..7143
                     /locus_tag="JQH65_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAY6139561.1"
                     /translation="MTEDPCEDDFPVTTLKISANSQSFQSEIQRASRMGSEYYRTLQN
                     GGRHAAAAAREQRRALAELNSQLTEIRGSAVGMAGAFAGAFATGHLISLADEWGSVNA
                     RLKQASQSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVT
                     EAISTGLKLSGAGVAESGSVITQFSQALAQGVLRGEEFNAVNENGDRVIRALAAGMGV
                     ARKDLKAMADDGKLTADKVVPALISQLGILRDEYAAMPETVSGSITKVENAFMAWVGE
                     VNEACGVTKTLSGVLNGVAGQIDNVATAVGALVAVGVARYFGNMASGAMSATAGLVTA
                     ARNEVALAEAQFRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAATQERLNR
                     NIAARSAAQNALNSTTAVGSRLMSGALGLVGGVPGLVMLGAAAWYTLYQNQEQAWESA
                     RQYALTIDEIAHKTPSMSLPEASDNEGRTRAALTEQNRLIDEQASRVKSLQEKIAGYQ
                     YVLANPGWTTGDGFMINHLTSVKTVTEGLAQATEQLAVEQSRLALMQEKAQSIQDVLA
                     GLEDRRVALIRQQAAEQNKVYQSMLVMNGQHTEFNRLLGLGNELLQQRQGLVNVPLRL
                     PQATLDDKQQSALTKTERELALSRLKGEEKERVRLGYAADDLGFVGDPYQEARQRYIS
                     NALEAWRNNEANKPKSRGGKSETEKAEDSFSRLLKQQKEQLALAGQNTELAKLKYQTA
                     QGELKTLTEMQKQELLRNAALIDQQKIREQLRSREETLKNDNVAARASNEAELLGYGQ
                     GERLRERMRELQQIRDSFRQKDADLQSQYQTGDISEDFYRQALAQNAQYLSERLKEQE
                     AFYAESDVQRADWQKGLQEGFSNWVDNASDYASQAAQLATEGISGMVNNITEMLNGNK
                     VEWRSWASSVLQEISKVLMNAAIVNGIKTAANSMSGAGGFIGSIGSWLGGAVANAKGG
                     VYTSANLSAYSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAVGSMN
                     GSAGLVYSPVYHIAIQNDGTNGQIGPEAAGSLVQLIDQRVQAVMLSMRRDGGMLSG"
     gene            7136..7477
                     /locus_tag="JQH65_002162"
     CDS             7136..7477
                     /locus_tag="JQH65_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY6139562.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVARVRFGDGYEQRRPTGLNARL
                     KTFQAVFRVTDEATRRWLEEFLSWHGGYRAFLWRPPKHNRTVRVVCREWSVTDNARYS
                     DFSCTIEQVVN"
     gene            7477..8175
                     /locus_tag="JQH65_002163"
     CDS             7477..8175
                     /locus_tag="JQH65_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HAY6139563.1"
                     /translation="MQDIHEESLSESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRKYEAYPIDGSGFEMNGRGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSQLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWTYRSDECGYTGGAVADEFDKPTTDIRKDRCSKCMRGCELRRNV
                     GNFGGFLSINKLSQ"
     gene            8186..8929
                     /locus_tag="JQH65_002164"
     CDS             8186..8929
                     /locus_tag="JQH65_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY6139564.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVISTPEGERYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGEIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIDMPDFHREDDWWRNGQNLYLDNMAVT
                     GFYRVPLSSAPPGDILLCCFGASVPNHATIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSVWRHRHWHASAFTGICNDLAAASACM"
     gene            8827..9507
                     /locus_tag="JQH65_002165"
     CDS             8827..9507
                     /locus_tag="JQH65_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HAY6139565.1"
                     /translation="MATTNAFCLASPPLARICLHGDLQRFGRRLSLYVNTAAEAIRAL
                     SMQMPGFRRQMNEGWYQIRIAGDDTAPEAVYARLHKQLGEGTVIHIVPRLAGAGKGGL
                     QIVLGAAAIVGSFFTAGASMALWGSALAAGGFSATTMLFSLGASMILGGVAQMLAPKA
                     KTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGGGK
                     VVVIGRQG"
     gene            9748..13221
                     /locus_tag="JQH65_002166"
     CDS             9748..13221
                     /locus_tag="JQH65_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HAY6139566.1"
                     /translation="MGKGGGRAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPESFESSGSETVLGVEVTRAKPVTR
                     TITSANIDRLRVTFGVQSLVETTSKGDRNPSSVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQFLASVILDNLPPRPFNIRMVRETADSTTDQLQNRTLWSSYTEIIDVKQCYPNTAIV
                     GMQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCL
                     WDMLTHPRYGMGKCLGAADVDKWALYAIGQYCDQTVPDGFGGTEPRMTFNAYLAQQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYTNSDVVVDDNGVGFRYSFSAL
                     KDRHTAVEVNYTDPQNGWQTSTELVEDPEAILRYGRNLLKMDAFGCTCRGQAHRAGLW
                     VIKTGLLETQTVDFTLGSQGLRHTPGDIIEICDNDYAGTMTGGRVLSIDAATRTLTLD
                     REVTLPETGTSAVNLINGSGKPVSVDITAHPAPDRIQVSTLPDGVETYGVWGLSLPSL
                     RRRLFRCVSVRENTDGTFAITAVQHVPEKEAIVDNGARFEPQSGSLNSVIPPAVQHLT
                     VEVSAADGQYLAQAKWDTPRVVKGVRFSLRLTSGSGEDSRLVTTAITADTEHRFSGLP
                     LGEYTLTVRAINSYGQQGEPATTTFRINAPAKPATIELTPGYFQITAVPRLAVYDPTV
                     QFEFWFSEAKIADTSQVETSARYLGTGSQWSVSGPHIKPGKDFWFYVRSVNLVGKSAF
                     VEASGRASNDAEGYLGLFREKIGKLHLAQGLWELIDNSQLADEMAEMKTTITETRNEI
                     TQTVSKTLEDQSATIQQIQRVQKDTNDDLAALYMLKVQKTKNGIPYVAGIGAGIEDTD
                     GQPLSNILLLADRIAMINPEDGNTTPLFVAQGNQLFMNDVFLKRLFAVSITSSGNPPT
                     FSLTPEGRLTARNADISGNVNANSGTLNNVTINENCRVLGKLSANQIEGDLVKTVGKA
                     FPRDSRAPERWPSGTVTVRIYDDQPFDRQIVIPAVAFSGAKHEREHTDIYSSCRLIVR
                     KNGAEIYNRTALDNTLIYSCVIDMPAGHGHMTLEFSVSAWLVNNWYPTASISDLLVVV
                     MKKATAGISIS"
     gene            13289..13888
                     /locus_tag="JQH65_002167"
     CDS             13289..13888
                     /locus_tag="JQH65_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HAY6139567.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLHARTNAPG
                     SDNLNGINVKYRYEFTDALGLITSFSYANAEDEKKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            13953..14949
                     /locus_tag="JQH65_002168"
                     /pseudo
     CDS             13953..14949
                     /locus_tag="JQH65_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216474.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S74"
     gene            14962..15360
                     /locus_tag="JQH65_002169"
     CDS             14962..15360
                     /locus_tag="JQH65_002169"
                     /inference="COORDINATES: protein motif:HMM:NF025253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HAY6139568.1"
                     /translation="MLTTANTWTQAQTFNGGINGNLTVTGNGSFNDIQIRSDKRNKRN
                     LVKLDNALDRLEALTGYLYEIQYSADGWQTSVGLIAQDAQKALPELVTEDADVISGEK
                     RLRLNYNGIIALLVEGFKTLRHEIKELREK"
     gene            15446..15958
                     /locus_tag="JQH65_002170"
     CDS             15446..15958
                     /locus_tag="JQH65_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6139569.1"
                     /translation="MGVTSGWVGSSAKSETGEQWMGAAGTKLGLSKPFMMSQMVGRTM
                     GCKIATEYYKWKSSDKVDNWGAVGADWPLEEKSKGTITNAASCGSGRLVGAVVTLSHF
                     LTNSTPTAAVYLAGGKAVNITVNVGGATQTMIYQGVVSGFQYYWSGSVSSAFVEAMKK
                     TGVPQDLKIS"
     gene            16034..16246
                     /locus_tag="JQH65_002171"
     CDS             16034..16246
                     /locus_tag="JQH65_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HAY6139570.1"
                     /translation="MPEKFFRTDADNNDVPMTAASWMALSEATEQAMFAKGVEINTRQ
                     LQMKAEVEALTDLKAIRSYVVGWPAG"
     gene            complement(16388..16713)
                     /locus_tag="JQH65_002172"
                     /pseudo
     CDS             complement(16388..16713)
                     /locus_tag="JQH65_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            complement(16849..17097)
                     /locus_tag="JQH65_002173"
     CDS             complement(16849..17097)
                     /locus_tag="JQH65_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HAY6139571.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(17452..>17798)
                     /locus_tag="JQH65_002174"
     CDS             complement(17452..>17798)
                     /locus_tag="JQH65_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAY6139572.1"
                     /translation="PAKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRR
                     RGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIA
                     RVRSVEEILHAL"
BASE COUNT         4197 a         4290 c         5436 g         3875 t
ORIGIN      
        1 cctggcggaa gtgcgtcagc acacctggtc atccggtacc aacggtgtgg tggctgcaac
       61 gttctcactg cgtctgaaag gcaaaccggt gtcctttgtg gtaccgctgg cgtttgtgaa
      121 aaatctggat aagacactta ccgtgaatac cggtgcgctg ctgacaatgt cagtcagtgc
      181 caacggggga atgccgccgt ataaatacgc ctggaagaag gatggtcagc cggttgacgg
      241 gcagacgaca gacaccttca gtaagccagg tgcgcagtcc gctgatgcgg ggaaatatac
      301 ctgcgtggtg accgattcgg cagagaaagc acagagtgtg acgtctgttg aatgcaccgt
      361 gacagtgagc gcagctgccg gataagggga tgggtcatca tgaaaaagga tctgaaaacg
      421 ctggcgctgg ccagactgtc agggtttcgt cataaaacgg tgaaggtgcc ggaatggggg
      481 aatgtcagcg tggtgctgcg ggagccttcg gcagaggcct ggtatctgtg gcaggaagtg
      541 ctcaatggtg atggagagga tgacgatacc ctgtcggtgg tggcgaaaac ccgccgtaac
      601 ttggaagcgg atgtgacgct gttctgcgat gtcctgtgtg atacggacct gcagcgggtg
      661 ttcactccgg acgaccgtga gcaggtgctg gccgtctatg gtccggtaca tgcccggttg
      721 ctgcgtcagg cactggaact gatcgctgat gcagagtcgg ccagaaaaaa gtagcccgcc
      781 cggaaattcg ctttctgatg cgacttgcgc tccgtctggg gcgcacctta tccgaactgc
      841 gccacagcct gagtgcgagc gaggcgatga tgtggatgga gttcgacagg atatccccgc
      901 tgggtgatga gcgcggggat atccgtaatg cacagatcgt gaaagcggtt ttcggggcac
      961 aggggatgaa tgttgcactg aaggacgtca tgctctgctg gggcgaggat gaggataagc
     1021 cggaggtgga tccgtttgcg gcgctggaag acgcgctgag ctttgcagca cagtcatgaa
     1081 tgatgagaac cgctgaggcg gtttttttac gcccggagaa aggtgaatgg cgacgttacg
     1141 tgaactgatt atcaaaattg taagcttccg gggaactcat attgacgcgt ttttcaggcg
     1201 cgcgtcatcg gcggcgcata cgaacgctcg tggtttgata acggttgatt tattttgttg
     1261 cacagaggcg atcgccaggc aggtaccgga ggcgggctct gaagtactga gtagcgtgat
     1321 cgcgtcaggc cgcgaagtac tgactcgggg attaattgca ggtgatgatg tactgacccg
     1381 ggaatgactc cgggctgatt caggttctta ttcgctgtca gcccgcgtgg ttgctggtct
     1441 ggctcatttc ctgccgtcag gagttcaact cccgactgca ggttatggcc cggtgcacga
     1501 acacctgacg gcaggtatca ctcacccgga gaaggtaaat ccctcaagag gcagcaactc
     1561 tgccagtcgc tcctccggcc actccggcag acgcatcagg acgtctgtca accaggcatg
     1621 cggctccaga ccgttgcgtt tcgcggtttc cagcaagctc attatttgcg cggcgcgttc
     1681 tcccgccatc tgcgaaccgg cgaacagcca cgattttctg cccagaacca cgtttttgat
     1741 ggcccgttca cacacattat tatccagcgg caccgcacca tcttccagga agcggctcag
     1801 ttccacgcga tgagacagcg cataggcaat agctttgtgt aatgcctttc ccggagaaca
     1861 ttgcggttcc tgctcttcaa gccatgacca cagttcttcc agtatcggac gggcataacg
     1921 ctgtcgccac tggcggattt tttccacggg gcggctactg atcttcttct caagacggta
     1981 caaccccgcg attttcttca cggctatggc agcccgtgga tctttactga ttttatacag
     2041 gtcggcgaag cccctgcggg catgggccca gcatccggcc agcgtgatct ccggcacttt
     2101 gtttgccaga gtccggtaag ctttatgtcc gtcgacaacc agcgtcccgc cccagccctg
     2161 aagccagttt tcaggatact catggctacg tccggtccgg cagtcgaagc acacaactga
     2221 cggtcccgtc ctttctccac tgacgtatgc ccacagataa ccggagcagg atttaccgcc
     2281 tttcttcgtg ttcaggatct tcagggtagt ctcatctgca tgcaccaccg gacggtttat
     2341 caactcgcga tggagtaacg ccgccagggg agataatgcg gcacccgccg cgccaaccat
     2401 gtcagccatc gaactgacgg gcagcccgac atctgaacgg gcaaagacct gttgctggcg
     2461 atacagaggc aggtggtcac cgtatttatt gatgatcacc tgtgcgatga ctgatggctc
     2521 aacggcactt ttcgggagga tatgtgccgg catttcaccg ctgaacacct tctggcaaca
     2581 gggacaactg tattgcggac ggacataacg gttcaccaca aagtgagcgg gaatatactc
     2641 cagcttttca ctcaccgcat cgcggatata gtgcagcggc tcgtcacatt cagggcaatg
     2701 gtcagtgtcc ggctggatca ttttttccac ccgtggaaga tgaaccggta agggtttgcg
     2761 tatcgggcgt gaacgggacg ctttttcatc ttcttcaggg gattgcggga gcagtttatc
     2821 cagatgtgcg gtaagcgcgg cgatatcggc atcaacatcc tcttcgaata gggagcgttg
     2881 cataccagcc agcgtttcac atttttttcc gaagcgctgt tgacgaacca gtttcagcat
     2941 ttcttccaga agctggatgc gccggctttt ctccagtaat tcccgctctt tttcggcatt
     3001 ctccgacatg acttcttgta ccatggcaag tgccatagca cgcagttttt cgatgtcatt
     3061 cgtggtgttg agagcggaga tatccataca ggggagtata ccactccaga tacggatatt
     3121 cattattttt cactgagtta gcagtgtatt tttcgtgttt tcagggcgca gttcaggttc
     3181 attctgccag acccatttcg tcaggtcatg tcccttaacc tgctgccagt caacaccggc
     3241 aatcagccag ttaaactcgt cgggagtgag gtgccaggca gcgtcatttg cacgtggcca
     3301 gcggaagctg cctttgtgca gacggcgggt acacaaccac accccgtgtt tatcccaccg
     3361 gagaactttg atacgcgaac ggactttgtt aacaaagacg aaggcggctc cctcatgcca
     3421 tgtcgaccgg agttcatcct gtatatactg cgtcagtgag tcgatgcccc ggcgcatgtc
     3481 aacaggttta attgcgatga agacattatc aggactgagc atatttgagt gccctgaaga
     3541 catcggtaag ttgtgacggc tgacagcaca tccgtactcc accgggaagg aagagcgtga
     3601 ctggctcatt cgtgacgggc tgtggtacat cagcatgcag tggtggcgca atatgaacgg
     3661 gaagaacggt cggttcattt gcccggcgtt cagcttttgc gacatcctgc ggccattcgc
     3721 gaagtgatgt gaagggaata tcgttgagtt cacagtattg ctgacgtgta agaccactgg
     3781 cgcgccaggc agcaacgtgc tgccttttct gttcaatggt ccatcggggt tttgacatcg
     3841 attatctccg gtaagttggt gaatggatgt cggtaatact aactgaccga agatccctgt
     3901 gaagatgact tccccgtaac gacactcaaa atttcggcaa attcacagtc attccagtcg
     3961 gagatccagc gggcgtcccg tatgggcagt gaatattacc ggaccctgca gaatggcgga
     4021 cgtcatgccg ctgctgccgc acgggaacag cgccgcgccc ttgcagaact gaacagtcag
     4081 ttgacggaaa ttcgcggttc tgctgtcgga atggctggcg catttgccgg tgcctttgcc
     4141 accggacacc tgatttcgct ggccgatgag tggggttccg tgaatgcccg tctgaaacag
     4201 gcatctcagt catcggatga attctcgtca tcacagaaag tgctgatgga tatcagtcag
     4261 cgaacaggta ccgcgttttc ggataatgcg gccctgtttg cccgttcggc tgcctcgatg
     4321 cgtgaatatg gttacagtgc tgatgatgtg ctgaaggtga cggaggccat ttcgacagga
     4381 ctgaaactgt caggggctgg agttgcggaa tccggttcgg tgatcaccca gttcagccag
     4441 gcactggcac agggtgtact gcgtggcgaa gaatttaatg ctgttaacga aaatggcgac
     4501 cgggtgatcc gcgcgcttgc tgcggggatg ggtgtggccc gtaaagatct gaaggcaatg
     4561 gcggatgacg gaaaactgac agcggataaa gtggtccctg cgttaatcag ccagctgggg
     4621 atattacgtg atgaatatgc ggccatgccg gaaacggttt ccggtagtat cacgaaggtg
     4681 gaaaacgcct ttatggcctg ggtgggcgaa gtgaatgagg cctgcggggt gacaaaaacg
     4741 ctctccggcg tgctgaacgg tgttgccgga cagattgata atgtggcaac agctgtgggg
     4801 gcgctggttg ccgtcggggt tgcccggtac tttggcaata tggcctccgg agcgatgtct
     4861 gccacggcag gacttgtgac ggctgcacgt aatgaagttg cactggcgga agcacagttc
     4921 aggggaacgc agattgccac ggcgcgggca agggcagccg tgtaccgtgc tcagcaggcc
     4981 gtggcggcag cccgcgggac ggagatgcag attgctgcag aggcccgtct ggcggccaca
     5041 caggaacgcc tgaacagaaa tattgctgcc agaagcgccg cccagaatgc gctgaacagt
     5101 acaacggcgg tgggctcacg tctgatgagc ggtgcgctgg ggctggttgg tggcgtaccc
     5161 ggactggtga tgctgggggc tgcagcatgg tacacgctgt accagaatca ggagcaggcc
     5221 tgggagtctg cgcgccagta tgcactgacg atagatgaaa tcgcgcataa aacgccgtca
     5281 atgtctttgc ctgaagcctc agataatgaa ggacgaacac gggcggcgct gacagagcag
     5341 aaccggctga ttgatgaaca ggctagtcgg gtgaaatccc tgcaggaaaa aatcgcagga
     5401 tatcagtatg ttctggcgaa cccgggctgg acgaccggtg acggattcat gataaaccat
     5461 ctgacctcgg tgaagaccgt aacggaaggg cttgctcagg caacagagca gcttgccgtt
     5521 gagcagtccc gtctggcact gatgcaggaa aaagcgcagt ccattcagga tgtgcttgcc
     5581 gggctggaag accgtcgtgt ggcgttaatt cgtcagcagg cggcagagca gaataaggtg
     5641 taccagtcca tgctggttat gaacggtcag catacggaat tcaaccgtct gctggggctg
     5701 ggtaatgaac tgcttcagca gcggcaggga ctggtgaatg tgccgttacg gctgccacag
     5761 gccactctgg atgataaaca gcagagtgcc ctgacaaaaa cagagcgtga gctggccctg
     5821 tccagactga aaggggaaga aaaagagcgt gtccgactgg ggtatgcggc ggatgacctc
     5881 ggttttgtgg gtgatccgta tcaggaggcg agacaacgtt atatcagtaa tgccctggaa
     5941 gcctggcgca ataacgaggc gaataaaccc aaatcccggg gtggaaaatc agagacggaa
     6001 aaagcggaag acagtttttc ccggctgctg aagcagcaga aagagcaact ggcactggcg
     6061 gggcagaata cagagctggc gaagctgaaa taccagactg cgcagggcga actgaaaacc
     6121 ctgacggaga tgcagaagca ggaactgctg cgcaatgcgg ccctgattga ccagcaaaaa
     6181 atccgggagc agttgcggtc ccgggaagag accctgaaga atgataatgt ggctgctcgt
     6241 gcatcaaatg aagcggaact gctggggtac gggcagggag agcggctccg tgaacgcatg
     6301 cgggagttgc agcagatccg cgacagtttc cgccagaagg atgcggacct tcagtctcag
     6361 tatcagaccg gggatatcag tgaggatttt tacagacagg ctctggcaca gaacgcgcag
     6421 tatctgagtg aacgtctgaa agagcaggaa gccttttatg ccgaatcgga tgtgcagcgt
     6481 gcggactggc agaaagggct gcaggaggga ttcagtaact gggtggataa tgcgtccgat
     6541 tacgcctcac aggcagcaca gcttgcgacg gagggtatct cagggatggt gaataacatc
     6601 acggagatgc tgaacggaaa taaagtggaa tggcgcagct gggcctcatc agtgctgcag
     6661 gaaatatcaa aagttcttat gaatgccgcg attgtcaacg ggatcaagac ggcggcaaac
     6721 agtatgtccg gagcgggagg atttatcggc agtattggta gctggctggg cggtgcggtg
     6781 gccaatgcaa aaggcggtgt gtatacctcg gcaaacctga gtgcgtacag caacagtatt
     6841 gtggatacgc ccacgtactt tgcctttgca aaaggggcgg gactgatggg ggaggccggt
     6901 cctgaagcca ttatgcccct gacccgggcg gcggatggct cgctgggtgt gcgagcggtg
     6961 ggcagtatga acggcagtgc aggtctggtg tattccccgg tctaccatat cgccattcag
     7021 aatgacggga ctaatggcca gatagggccg gaagctgcgg gcagccttgt gcaactgatt
     7081 gaccagcggg tgcaggcggt gatgctgtcc atgcgacgtg acggaggaat gctgagtggc
     7141 tgagataaaa acgctgcatc tggtcccgcg tgaagggatg caggtgagtg agaaaccgtc
     7201 ggtggcgagg gtacggtttg gtgacggtta tgaacagcgc cgcccgacgg gacttaatgc
     7261 ccgactgaag acgtttcagg cggtgttccg ggtgacggat gaggcgaccc ggcgatggct
     7321 ggaagagttt ttatcgtggc atggtggtta ccgtgccttt ttgtggcgac cgccgaaaca
     7381 taaccggacg gtgagggtgg tatgccggga gtggagcgtc acagataacg ccaggtacag
     7441 tgatttcagt tgtacgattg agcaggtggt gaactgatgc aggatattca cgaagaaagt
     7501 ctgagcgagt cggttaaatc agagcagtca ccgcgggtgg tactctggga aattgacctg
     7561 acggtgcagg gtggtgagcg gtattttttc tgtaatgagc tgaatgaaaa aggggagccg
     7621 gtcacctggc aggggcgtaa gtatgaggca tacccgattg acggcagcgg ctttgagatg
     7681 aacggccggg gcagcagtgc cagaccgtcg ctgacggtgt ccaatctgtt cggtctggtc
     7741 accgggatgg cggaagacct gcagagtctg gtgggggcca cggtggtccg ccgccgggtg
     7801 tatgcccgtt ttctggatgc ggtgaatttc gttgcgggca atccggaggc ggacccggag
     7861 caggagctga gtgaccgctg ggtggtggag cagatgtcgc agctgacagc catgacggcc
     7921 tcgtttgtgc tggctacacc gaccgagacg gacggggcgc tgtttcccgg tcgtatcatg
     7981 ctggcgaaca cctgtatgtg gacctaccgc tctgatgagt gtggttacac gggcggggct
     8041 gtggcggatg agttcgataa acccaccacg gatatccgta aggacagatg cagcaagtgc
     8101 atgcgcgggt gtgaactgcg caggaatgtc ggcaattttg gcggtttcct ttccattaat
     8161 aaactttcgc agtaaatccc gatttatgac acagactgaa tcagcgattc tggcgcatgc
     8221 ccggcggtgt gcgcctgcgg agtcgtgcgg cttcgtgata agcacgccgg agggggagcg
     8281 gtatatccct tgtgtgaata tttctgcaga gccggaggcg tattttcgta tcgcaccgga
     8341 agactggctg cgggcagaga tgcaggggga gattgtggca ctggtccaca gtcatcccgg
     8401 tggtctgccc tggctgagtg aggctgaccg gcggctgcag ataaaaagcg cactgccctg
     8461 gtggctggtc tgccgggggg aaattcataa attccgctgt gtgccacatc tgacaggacg
     8521 gcgctttgag cacggggtga cggactgtta cacgctgttc cgggatgcat accatctggc
     8581 gggaattgat atgccggatt ttcatcgcga ggatgactgg tggcgcaacg gccagaacct
     8641 gtacctggac aatatggcgg tcaccggctt ttaccgggtg cccctgtcct ctgcaccgcc
     8701 gggcgatatt ctgctgtgct gctttggtgc ttcggtaccg aaccatgcca ccatttactg
     8761 cggcaacggt gagctgcttc accatctgcc tgaacaactg agtaaacggg agaggtattc
     8821 cgaaaaatgg caacgacgaa cgcattctgt ctggcgtcac cgccactggc acgcatctgc
     8881 cttcacgggg atttgcaacg atttggccgc cgcctcagcc tgtatgtgaa cacggcagcg
     8941 gaagccattc gcgccctgtc gatgcagatg ccgggctttc gccgtcagat gaacgaaggc
     9001 tggtaccaga tacgtattgc cggtgatgac acggcaccgg aggcggtgta tgcccgtctt
     9061 cacaaacagc tgggtgaggg aacggtcatc cacattgtgc cgcgactggc cggggccgga
     9121 aagggtggac tgcagattgt gttgggggcg gcagccatcg tgggctcttt cttcactgcc
     9181 ggggcatcaa tggcgttatg gggttcagcc ctggcagccg gtggtttttc tgccaccacg
     9241 atgctgtttt cacttggagc cagcatgatt ctgggcggtg tggcccagat gctggccccg
     9301 aaggcaaaaa caccggatta ccgcgcaacg gataacggca gacagaacac gtacttttcc
     9361 tcgctggata acatgattgc ccaggggaac ccgatgccgg tgccttacgg ggaaatgctg
     9421 gttggctccc gccgtatatc ccaggacatc agcacccgtg atgaaggcgg gggcggaaag
     9481 gtcgtggtta tcgggcgaca gggataaaac ataaaaaaat cccgcagtga tcgcggagct
     9541 gcggggacag acaaatgaag atcaatgtga aggagttgtt tttgttactc gggcaaaaaa
     9601 acactaacgc agcgaaatta taagcgccac agtcagtgtg tgaaaatgtg aagatattca
     9661 gaaattttat tccgtcatga cgcaggcacc cggtgaggtg cctgttgttt ttgtgagtga
     9721 acaattatca cggtaagagg tgatgtaatg ggcaaaggtg gcggcagggc gcacacaccg
     9781 cgtgaggcga aagacaatct caaatccacg cagatgatga gcgtgattga tgcgattggt
     9841 gagggaccga tagaaggccc ggtgaaaggc ctgcagagta ttctggtgaa caaaaccccg
     9901 ctgacggaca cggacggtaa tcccgtgata cacggtgtga ccgcggtctg gcgtgccggg
     9961 gagcaggagc agacaccacc ggaaagcttt gagtcctccg gctctgaaac tgtactgggt
    10021 gtcgaagtga ccagggcaaa accggtaaca cgcaccatta cgtcagcgaa cattgaccgc
    10081 ctgcgggtga ccttcggggt gcagtcactg gtggagacca cgtcaaaggg tgaccgtaat
    10141 ccgtcctctg tccgtctgct gattcagtta cagcgtaacg gtaactgggt gacagaaaag
    10201 gatgtcacca ttaacggcaa gaccacctca cagttccttg cgtcggtgat tctggataat
    10261 ctgcctcccc gcccctttaa catccggatg gtcagggaga cggcggacag caccacggac
    10321 cagctgcaga acagaacgct gtggtcgtca tacaccgaaa tcatcgatgt gaaacagtgc
    10381 tacccgaaca cggccattgt ggggatgcag gtggatgcgg agcagtttgg tggtcagcag
    10441 atgacggtga actaccatat ccgcggtcgc atcatccagg tgccgtcaaa ctatgacccg
    10501 gaaaaacgca cgtacagtgg tatctgggac ggcagtctga aaccggcata cagcaacaac
    10561 ccggcctggt gcctgtggga catgctgact cacccgcgct acggcatggg aaaatgtctg
    10621 ggggcggcgg atgtggacaa gtgggcgctg tatgccatcg ggcagtactg cgaccagacg
    10681 gtcccggatg gtttcggggg gaccgagccg cggatgacct ttaatgcgta cctggcacaa
    10741 cagcgtaagg cgtgggacgt tctcagtgat ttctgctctg cgatgcgctg tatgccggta
    10801 tggaacggtc agacgctgac gttcgttcag gaccgcccgt cggatgtggt gtggccgtac
    10861 accaacagcg atgtggtggt ggatgataac ggcgtgggat tccgctacag cttcagtgcc
    10921 ctgaaggacc ggcacacggc ggtggaggtg aattacaccg acccgcagaa cggctggcag
    10981 acctccacgg aactggtgga agacccggaa gccatactgc gctacggacg caacctgctg
    11041 aagatggatg cgtttggctg tacctgccgc ggtcaggctc accgtgcagg tctgtgggtg
    11101 ataaagaccg gactgctgga aacgcagacg gtggacttca cgctcgggtc acaggggctg
    11161 cgtcacacac cgggtgacat tattgaaatc tgtgataacg actatgctgg gactatgacc
    11221 ggcggacgtg tcctgtccat tgatgctgcc acccgcaccc tgacgctgga ccgtgaggtt
    11281 accctgccgg agacaggtac atcggcggtg aacctgatta acggcagtgg taagccggtg
    11341 agtgtggaca tcaccgcaca ccccgcgccg gaccggatac aggtcagtac cctgcctgat
    11401 ggtgtggaga catacggggt gtggggactc tccctgccgt cactgcgccg tcgcctgttc
    11461 cgctgtgtct ccgtccggga aaacacggat ggcacctttg ccatcacggc ggtgcagcac
    11521 gtaccggaaa aagaagccat cgtggataac ggtgcccgct ttgagccgca gtcaggctcc
    11581 ctgaacagcg tcatcccacc ggcagtgcag cacctgacgg tggaggtgag cgcagctgac
    11641 ggccagtatc tggcacaggc gaaatgggac acgccgcggg tggtgaaggg ggtgcgcttc
    11701 agtctgcgac tgaccagcgg aagcggagaa gacagccgtc tggtgaccac cgctatcact
    11761 gcggatacag agcatcgttt cagtggtctg ccgctcgggg aatacaccct gacagtcagg
    11821 gcaattaaca gttatggcca gcagggcgaa ccggccacca ccaccttccg gattaacgcg
    11881 ccagcaaaac ccgccaccat tgaactgacg ccggggtatt ttcagataac agcggtaccg
    11941 cgtcttgcgg tgtatgaccc gacggtacag tttgagttct ggttttcgga ggcaaaaatc
    12001 gcagacacat ctcaggtgga aacctctgcc cgttatctgg ggaccggcag tcagtggagt
    12061 gtatccggcc cgcacattaa gcccgggaag gatttctggt tttacgtgcg cagcgtcaac
    12121 ctggtgggga aatctgcttt tgtggaggcc agtggacggg ccagcaatga tgcagaaggg
    12181 tatctggggc tgtttcggga aaaaatagga aaactgcatc tggctcaggg gctgtgggag
    12241 ctgatagaca acagccagct tgcggatgag atggcggaga tgaagaccac catcaccgaa
    12301 acccgcaatg aaatcacaca gacggtcagt aaaacgctgg aggaccagag cgccaccata
    12361 cagcagatac agcgcgtgca gaaggacaca aatgatgacc ttgctgcact ttacatgctg
    12421 aaggtacaga aaacaaaaaa tggcataccc tatgttgccg gtattggagc ggggattgag
    12481 gatactgatg gccagcccct gagcaacata ctgctgctgg ctgaccgtat tgcgatgatt
    12541 aacccggagg acggcaacac cacgccgtta tttgtggcgc aggggaatca gttgttcatg
    12601 aacgatgtgt tcctgaagcg gctgtttgcg gtgagtatca cctcgtccgg caatcccccg
    12661 acgttttccc tgacgccgga gggcaggctg accgcaagaa atgctgatat cagcggtaac
    12721 gtgaatgcga actccgggac gctcaacaac gtcacgatta acgagaactg ccgggttctg
    12781 ggaaaactgt ccgcgaacca gattgaaggc gatctcgtta aaacagtggg taaggctttt
    12841 ccgcgggact cccgggcacc ggagcggtgg ccatcaggga ccgttaccgt caggatttat
    12901 gacgatcagc cttttgaccg gcaaattgtt attccggcgg tggcattcag cggcgctaag
    12961 catgagagag agcatactga tatttactcc tcatgccgtc tgatagtgcg gaaaaacggt
    13021 gctgaaattt ataaccgtac cgcgctggat aatacgctga tttacagttg cgttattgat
    13081 atgcctgccg gtcacggtca catgacgctg gagttttcgg tatcagcatg gctggtgaat
    13141 aactggtatc ccacagcaag tatcagcgat ttgctggttg tggtgatgaa gaaagccacc
    13201 gcaggcatca gtatcagctg aattttataa cccatatacg ggcgccagaa atggcgcctt
    13261 ttttattgca gaaaagcgag aggtaattat gcgtaaagtt tgtgcagcca ttttgtccgc
    13321 agccatctgt ctggctgtat ccggtgcgcc tgcatgggcg tctgaacatc agtccacact
    13381 gagcgcgggg tatcttcatg cccgtacgaa cgctcccggc agcgataatc tgaacgggat
    13441 taacgtgaaa taccgttatg agtttacgga cgcgctgggg ctgattacgt ccttcagtta
    13501 tgccaatgct gaagatgaga aaaaaacgca ctacagcgat acccgctggc atgaggattc
    13561 cgttcgtaac cgctggttca gcgtaatggc ggggccgtct gtgcgcgtga atgaatggtt
    13621 cagcgcgtat gcgatggcgg gtgtggctta cagccgtgtg tcgactttct ccggggatta
    13681 tctccgcgta actgacaaca aggggaaaac gcacgacgtg ctgaccggaa gtgatgacgg
    13741 tcgccacagc aacacgtctc tggcgtgggg ggctggcgtg cagtttaacc cgaccgaatc
    13801 cgtggccatt gatattgctt atgaaggttc cggcagtggc gactggcgca ctgacggttt
    13861 catcgtgggt gtcggctata aattctgatt agccaggtaa cacagtgtca tgacagcccg
    13921 ccggttcagg cgggcttttt tgtggggtga atatgacagt aaagatttct ggtgtactga
    13981 aagacggcac aggaaaaccg gtacagaact gcacaatcca gctgaaagca aaacgtaaca
    14041 gtaccacggt ggtggtgaac acgctggcct cagaaaatcc ggatgaagcc gggcgttaca
    14101 gtatggacgt tgagtatgat cagtacagcg ttattctgtt ggtggaggga ttcccgccgt
    14161 cacatgccgg gactatcacc gtgtatgaag attctcaacc cggtacgctg aatgattttc
    14221 tcggtgccat gactgaggat gatgcccgtc cggaggcact gcgtagcttt gaactgatgg
    14281 tggaagaggt ggtgcgtaac gcagaggagg cgaagaagaa tgccggagag gcggagacgt
    14341 cagcgaggaa tgccggcata tcagccagtc aggcagaaga gagcgctgca aatgctgaca
    14401 cttcagcagg ggatgcatcg gagtcagccc ggcaggcggc agaaagtgca gcctcagcaa
    14461 agcagtcaga ggaggcgtcc tcgtcctcgg cctctgcggc cgctcaaaaa gccagtgagt
    14521 cattacaaag tgcaacagat gctgagttgt caaaaaagac ggcagaaagt gcagccggta
    14581 atgcagtcag ggatgcaacg accgcaacag aaaaagcccg ggagtcagca gaaagcgcac
    14641 agtcagcgga acaaagcagg atagcggcgg aagaggccgt aaaccgaatc cccaccgtgg
    14701 tgggacctcc cgggccaaag ggggaaccgg ggcccgcggg tcctcagggg ccgaagggag
    14761 ataaaggaga gcgtggcgac accggcccgg caggggccaa aaggtgacaa aggcgatccg
    14821 ggagtggctg gaccagcagg tccggcaggt gcgccggggc cgaaaggcga taaaggtgat
    14881 ccgggagtag caggtccagc aggtccggcg gggccacagg gaccgaaggg agacacagga
    14941 gcccccgggc aaggaacaga actgcttact actgccaata catggactca ggcacaaact
    15001 tttaatggtg gtattaatgg caatttgacg gtgaccggaa acggctcatt taacgatatt
    15061 cagatccgtt cggataaacg caacaagcga aatctggtaa aactggataa tgcgttagat
    15121 cgtctggagg cacttactgg ttatctttac gagatacagt actctgccga cggttggcaa
    15181 acgtcggttg gtttaattgc tcaggatgca caaaaagcat tgcctgaact ggtaactgaa
    15241 gacgcagacg ttatatctgg tgaaaaacgt ctgcgtctta actacaacgg cataattgca
    15301 ttgttagtcg agggctttaa aacacttcgt catgagatta aagaactccg ggagaagtaa
    15361 acgacagctg ttgtagtttc tggtttctac tgaatttaaa ttgtggggtg acactcaccc
    15421 cacgcattca gaagggggag atgagatggg ggtaacatcg ggatgggtag gctcttcggc
    15481 taagagcgaa acaggtgagc agtggatggg agccgccgga accaaactgg gactgagtaa
    15541 accttttatg atgagccaaa tggttgggcg aactatgggc tgtaaaattg caactgagta
    15601 ctataaatgg aaatcctctg acaaggttga taactggggc gcagttggcg ctgactggcc
    15661 tttagaagaa aaaagtaaag gtacaattac aaacgccgca agctgcggat cagggaggct
    15721 ggtaggggct gtcgttacac tttctcactt tttgacgaac tctacaccga cagctgctgt
    15781 ttatttagcc ggtggtaaag cagttaacat caccgtaaac gtaggtggtg ctacacaaac
    15841 catgatttat cagggcgttg ttagtgggtt ccagtattac tggtcaggtt ctgttagttc
    15901 cgctttcgtg gaggcaatga aaaagacggg agtaccccag gatctaaaaa ttagttaaat
    15961 ggtaaataaa taattttaaa aacttcacgt tctacatacc ggagacgccg gatatatagg
    16021 atattgtttt aagttgccag agaaattttt ccggacggat gctgataata atgatgtgcc
    16081 aatgacggca gcatcatgga tggcactgag tgaagcgaca gagcaggcga tgtttgcgaa
    16141 gggagtggag attaatacac ggcagttgca gatgaaagcc gaggttgagg cgctgacaga
    16201 cctgaaggcg atccgcagtt atgttgtcgg atggcctgct ggctgaataa aaaaacggga
    16261 ccacgaccag tcccggaacc atgagtttta gggtattagt ttgttatcat agttagcgtg
    16321 ctaagtatgc catgtcaggt tgattagtga agtgatgttg ttcgcatttt tgcacggcgg
    16381 agaatattca gatttttgga aatccatatt tttcccgtgc gcgattacat gcttcattta
    16441 cgatgctatt ttcgccggac attgcgaatt ccctgcatgt ggacggtttt tgtaaacaga
    16501 gcaatatgcg ttttctccgg ggggggccgg caagagcgcc acatcgggga tttttctgat
    16561 tggtgccgcg catacagcga tgaaaaggag atatttgctc agtgagactg accgaaacct
    16621 ttccgccagc atcgtcagct tctgcccagt aaaaagagac gcggaaaatg cacaacaggc
    16681 accacacgtc atgcatggat tcagattgct cataattcac cagtacagct ataaatcgta
    16741 aagagaacag cggtacatcg tatgtaagaa cgtaccgcgg ctggctggtg aacttccgat
    16801 agtgcgagta ttgaatgatt tccagccgtt accgatttta cgtgctaatt agtgaacaaa
    16861 ccactcgtca gcagactccc aggtatcttt cagagtttcc tgaacaaaag ttttagctga
    16921 atctttatcg gcggtgcgcg taacagaaag gccatcgttg ctggtagctt ttacgatcac
    16981 ctctacatcg tcataacgtt tactgatgcg tcgggttaat tcttccttta acgcatccac
    17041 agcaccggtt ggcattttag tcattttttc tttggctatg cagatttcaa tacgcataaa
    17101 agtccctcta cactgtgttt gtatacagta tcatttttaa ctgtatggat aaacagtatt
    17161 aagggtgaag gaaagtgatt ttttggggca tgcatggggc aaaaatattt gttgtggggc
    17221 attttggggc atgattggga cactttgtta catatgaact ttgccgattt ttatatgaca
    17281 tggaaataca acgctttgat tcttctaaat aaaaaacatg ctcttgggcg ttctttagtg
    17341 atttttaaaa attccgtatc acgcagttaa agtggcgggc atactcttca aggctggtga
    17401 tccccaaccg cacccattta ggatgcgacc attgcggtag accgatgtaa atcataacgc
    17461 gtgaaggatc tcttccacgc tacgcacacg ggcgatgcgc gggtagatat gattaatgct
    17521 gttattgtgt tgctcggcgc tggcggcact acaggcgtct tcggcaatca ccagattaaa
    17581 gccaagttcc caggcattgc gggcggtgga ttcaacaccg atattggtcg agatcccaca
    17641 taacactatt gtatcgatac ctcggcggcg cagttgcaac tccagatccg taccgtaaaa
    17701 cgcaccccac tgacgtttga tgatttcgat atcactgtca gttgcaccta atgcagctgg
    17761 atgttgccac cagttttcgg gcaacacttt agcggggg
//
LOCUS       DADLFA010000076        17692 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000076 DADLFA010000000
VERSION     DADLFA010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17692
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.076"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..224)
                     /locus_tag="JQH65_002175"
     CDS             complement(<1..224)
                     /locus_tag="JQH65_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HAY6139573.1"
                     /translation="MTWNLLALATALQTVPEQNIDVTNSENALIIKMNDYGDLQINIL
                     FTSRQMIIETFICPVSSISNPDEFNTFLLR"
     gene            complement(351..1082)
                     /gene="rlmB"
                     /locus_tag="JQH65_002176"
     CDS             complement(351..1082)
                     /gene="rlmB"
                     /locus_tag="JQH65_002176"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAY6139574.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     AQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLVRT
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(1263..3704)
                     /gene="rnr"
                     /locus_tag="JQH65_002177"
     CDS             complement(1263..3704)
                     /gene="rnr"
                     /locus_tag="JQH65_002177"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAY6139575.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAECRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRLVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3743..4168)
                     /gene="nsrR"
                     /locus_tag="JQH65_002178"
     CDS             complement(3743..4168)
                     /gene="nsrR"
                     /locus_tag="JQH65_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HAY6139576.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4373..5671)
                     /gene="purA"
                     /locus_tag="JQH65_002179"
     CDS             complement(4373..5671)
                     /gene="purA"
                     /locus_tag="JQH65_002179"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAY6139577.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5775..5972)
                     /locus_tag="JQH65_002180"
     CDS             complement(5775..5972)
                     /locus_tag="JQH65_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HAY6139578.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(6054..7058)
                     /gene="hflC"
                     /locus_tag="JQH65_002181"
     CDS             complement(6054..7058)
                     /gene="hflC"
                     /locus_tag="JQH65_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HAY6139579.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(7061..8320)
                     /gene="hflK"
                     /locus_tag="JQH65_002182"
     CDS             complement(7061..8320)
                     /gene="hflK"
                     /locus_tag="JQH65_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HAY6139580.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTYPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8406..9686)
                     /gene="hflX"
                     /locus_tag="JQH65_002183"
     CDS             complement(8406..9686)
                     /gene="hflX"
                     /locus_tag="JQH65_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAY6139581.1"
                     /translation="MFDRYDAGEQAVLVHIYFTKDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9763..10071)
                     /gene="hfq"
                     /locus_tag="JQH65_002184"
     CDS             complement(9763..10071)
                     /gene="hfq"
                     /locus_tag="JQH65_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HAY6139582.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(10157..11107)
                     /gene="miaA"
                     /locus_tag="JQH65_002185"
     CDS             complement(10157..11107)
                     /gene="miaA"
                     /locus_tag="JQH65_002185"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAY6139583.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQAHDEVLQVVGAIAG"
     gene            complement(11100..12947)
                     /gene="mutL"
                     /locus_tag="JQH65_002186"
     CDS             complement(11100..12947)
                     /gene="mutL"
                     /locus_tag="JQH65_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAY6139584.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12957..14294)
                     /gene="amiB"
                     /locus_tag="JQH65_002187"
     CDS             complement(12957..14294)
                     /gene="amiB"
                     /locus_tag="JQH65_002187"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HAY6139585.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKHSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14313..14774)
                     /gene="tsaE"
                     /locus_tag="JQH65_002188"
     CDS             complement(14313..14774)
                     /gene="tsaE"
                     /locus_tag="JQH65_002188"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAY6139586.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14746..16293)
                     /gene="nnr"
                     /locus_tag="JQH65_002189"
     CDS             complement(14746..16293)
                     /gene="nnr"
                     /locus_tag="JQH65_002189"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HAY6139587.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLFAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLRQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16292..17431
                     /gene="queG"
                     /locus_tag="JQH65_002190"
     CDS             16292..17431
                     /gene="queG"
                     /locus_tag="JQH65_002190"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAY6139588.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSKSEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQPICPWNRYSQLTTEEDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17414..17467)
                     /locus_tag="JQH65_002191"
     CDS             complement(17414..17467)
                     /locus_tag="JQH65_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6139589.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         4070 a         5012 c         4390 g         4220 t
ORIGIN      
        1 tttcttaata agaaggtatt aaattcatct ggattgctga tactacttac cggacaaata
       61 aaggtttcga taatcatttg gcgggaagta aaaagaatat tgatttgcaa atcgccataa
      121 tcattcattt taataattaa tgcgttctcg ctatttgtta catcaatatt ttgttcaggt
      181 acagtttgca gcgccgtcgc cagcgccaac agattccatg tcatatatta ttccatatag
      241 atttagttta aatattaata aaatgaatat ttgcagtacg taattatctt accagctata
      301 gacaaaaaaa aaccatccaa atctggatgg cttttcataa ttcagaggaa ctagctgcgc
      361 tgacgcaccg cttcaaataa gcaaattccg gttgcaaccg aaacgttcag agaagaaaca
      421 cttcctgcca ttgggatgct gatcaactca tcgcaatgtt cacgggtcag gcgacgcata
      481 ccttcacctt ccgcgcccat caccagcgcc aggcgtccgg tcattttgct ctgatagagt
      541 gtatgatccg cctcacctgc cgtaccgacg atccagatat tctcttcctg caacatacgc
      601 atggtacgca ccaggttagt cacccgaatc agcggaacgc tttctgccgc gccacaggcc
      661 acttttttcg ccgtggcgtt gagctgggcg gagcgatctt tcggcacaat caccgcatga
      721 acaccagcgg catctgcgct acgcaggcac gcgccgaggt tatgcggatc ggttacaccg
      781 tcaaggatca gcaggaacgg ctgatcgagc gaagcgatca gatccggcaa atcattttcc
      841 tggtactgac gtcctggctt cacgcgggcg ataatgccct gatgcacggc accgtcgctt
      901 ttctcgtcga gatattggcg gtttgccagc tggataacca cgccctgggc ttcgagggcg
      961 tgaatcagcg gtaacagacg tttatcttca cggcctttta aaataaagac ttcctgaaaa
     1021 cgttcagggg cgcgctccag cagggcctgc actgcgtgga tgccgtaaat catttcgctc
     1081 attaatgtac tcgttgttta cgttttgcct gatgcactac gtttatccgg cctacatgat
     1141 ctctgcaata tattgaattt gcgtgattat gtaggccgga taaggcgttc acgccgcatc
     1201 cggcatgaac aaagcgcatt ttgtcagcaa tctaaccctc ttctgtttga agagggtaat
     1261 tatcactctg ccactttttt cttcgccgca cgcttcgctt tggtcgctgc agctattttc
     1321 tgcgttttcg ccgatggctt tttcgctttt ctcgcgtctt tcttcgctgc tttcggcttc
     1381 gtttttttct caccgcggaa ggcgctgtct ggctcaaagt ttaccttttt accgaccaga
     1441 cgacgcttgc cgcctttttt acctgcatcg ccttttttcg ctttctcgcg cgccgtttta
     1501 ccgacgttgc gcggtgcgcg ttcgctggag atcaggctaa agtcgatttt gcgctcgtcc
     1561 atattaaccg cttcgacgcg aacttccacg cgatcgccca gacgataagt ctggccgctg
     1621 gattcaccca tcagccgttg ccctacctgg tcaaagcgat agtagtcatt gtccagcgaa
     1681 gagacatgga ccagaccatc aatgaataag tcgtccagac ggacaaagaa tccaaagcca
     1741 gtgacgctgg aaattacgcc tttaaagacg ttacctacct ggtcgagcat gaagtcacac
     1801 ttcagccagt cagccacatc gcgcgttgct tcgtcggcac gacgttccgc catcgaacag
     1861 tgctgaccca gttgcagcat ttcttccatc gaataatggt agccgccggt ttcagtggtg
     1921 ttgccctgat gcccctgctc tttcgccagc agatatttaa tggcgcggtg cagcgtcagg
     1981 tctggataac gacgaatcgg cgaagtaaag tgcgcatagg actgcaatgc caggccaaag
     2041 tgaccacggt tttctggatc gtaaatcgcc tgtttcatcg agcgcagcag catggtttgc
     2101 agcatttctg catcaggacg atcggcaacc gactccagca gctccgcgta gtcacgcggt
     2161 tccggcttgt taccgcccgg cagttccagc cccagctccg ccagtactga acggaaagag
     2221 gtaatcgctt cggtgctcgg cttgtcgtga atacggaaca atgctggttc tttggctttc
     2281 tcaacgaaac gcgccgccga gatattcgcc agaatcatgc actcttcaat taacttgtgt
     2341 gcgtcgttac gctgagtctg ttcgatacgc tcaatacggc attcagcgtt gaaaataaac
     2401 ttcgcttctt cgctctcaaa tgagatcccg ccacgttctt cacgggcttt atccagcact
     2461 ttatagaggt tatgcaactc ttcgagatgc ttaaccagcg gagcgtactg ctcgcgcaga
     2521 tcctgatcgc cctgcagaat atgccagact ttggtgtagg tcagacgcgc gtgagagctc
     2581 atcaccgctt cgtagaattt gtagcccgtc aggcggcctt tcgacgaaac cgtcatctcg
     2641 cacaccatac acaggcggtc tacctgcgga ttgagcgagc acagaccgtt agagagcact
     2701 tccggcagca tagggataac ctgcgaaggg aagtacaccg acgtaccacg attacgcgct
     2761 tctctatcca gcggcgttgg cggacgcaca tagtagctga cgtcggcaat cgcgacccat
     2821 aaacgccagc cgccgccgcg ttttttctcg cagtaaaccg catcgtcaaa gtcacgggcg
     2881 tcttcgccat caatggtgac cagcggtaaa tcgcgcaaat cgacacggcc cgcttttgct
     2941 tcttccggca cttcttcttt cagcccggca acctgttgct caacagcctg cggccagatg
     3001 tacggaattt catgggtacg caaagcgata tcaaccgcca tgccggtgcc catattgtcg
     3061 cccagcactt cgacgatttt acccaccgct ttggtacggc gagtcgggcg ttgggtcagt
     3121 tcaacaacca ccacaaagcc catccgcgcg cccatgatct gatcgggcgg gattaagata
     3181 tcgaagctca gacggctatc gtcaggaacc acaaagccga cgcccgcttc ggtaaagtag
     3241 cgaccaacaa tctggctggt ttttggcacc agtacgcgga caatacgcgc ttcacgacga
     3301 cctttacggt cagcacccag cggctgagcc agcacctgat cgccatgaat gcaggttttc
     3361 atctgctcgc tggagagata caaatcatct ttacgccctt caacccgcag aaagccgtag
     3421 ccatcacggt ggccaataac ggtacctttc accaggtcga ggcgttccgg cagcgcatag
     3481 cactgacgac gagtgaagac cagttgacca tcgcgctcca tcgcgcgcag gcggcgacgc
     3541 aggccttcaa gctgctcttc gccttcaatg tgcagttcta ccgccagctc atcacggctg
     3601 gccggttttt cacgtttggt taaatgttcg aggataaatt cccgactagg gatgggattc
     3661 gcgtattttt cagcttcgcg ttcctggaaa ggatcttgtg acatctcggt tcctccgttg
     3721 tcatctctga tgaagatttt cgtcactcca ccagcaataa tttataaagc ggttgattct
     3781 cttcaaccaa atcggcaagc gtgtagttat ccagttccgt aagaaaactt tgcacggcct
     3841 tagaaagcgc ctgtttcaac cgacaggcag gtgtaatgtg gcaaaactca ctgctgcaat
     3901 tcaccagcga taagggctcc agctcgcgca ccacatcacc aatacgtatc gcactcgccg
     3961 gtttacccag gcgaatgccg ccattttttc cacgaacagc agtcacgtag ccggcacgac
     4021 taagttgatt gattattttg accatatgat tacgggagac gccgtagacg tcagtcactt
     4081 cagaaatact ggtcatccgc ccttctggca atgacgccat gtagatcagc gcacgtaatc
     4141 cgtaatcagt gaaactcgtt aactgcacat caacctcaaa agggaaatcg ggaaaaaata
     4201 catttatatt gatgataaac cagccacaag ctgtgtcgct aatttatttc agttcgggga
     4261 aggaaaaaag cgaggattta acactgtgtc ggatagcgta atggcttaac cgacacagca
     4321 atgtatcaca ctgttagccc ggcaggcaaa atatctgcca ggcgtaccag aattacgcgt
     4381 cgaacgggtc gcgcagaatc atagtttcag tacgatccgg accggtagag atgatatcga
     4441 tcggcacacc ggtcagctct tcaatacgct tgatgtagtt cagcgccgcc tgcggcagcc
     4501 cgctacgatc tttcacgccg aaagtggatt cagaccagcc cggcatggtt tcgtaaatcg
     4561 gctctacacc tttccagtcg tcagctgcca gcggagtggt agtcacttcg cgaccatccg
     4621 gcatacggta agccacgcag agtttaacct ctttcaggcc atccagaacg tccagtttag
     4681 tcaggcagaa gccagacagg gagttcagct gtaccgcacg acgaacggca acggtgtcca
     4741 gccagccggt acgacgacga cgacccgtcg ttgcgccgaa ttcgttaccc tgcttgcaga
     4801 ggaactcgcc agtttcatca aacagttcgg tcgggaaagg acctgcaccc acacgagtgg
     4861 agtaagcttt gaggataccc agaacgtaat caacataacg cgggcccagg ccggaaccgg
     4921 tcgccacgcc accagcagtg gtgttggaag aagttacgta cggataagta ccgtggtcga
     4981 tatccagcag cgtaccctgc gcaccttcaa acatgacgaa atcgccacgc tgacgcgcct
     5041 ggtcgagcag gtcagaaacg tcaaccacca tagaagtcag gatgtcggca acagccatcg
     5101 tatcatccag aactttctgg taatcaaccg cttcagcttt gtagtagtta accaactgga
     5161 agttgtgata ttccatcact tctttcagtt tttcagcgaa ggtttctttg tcgaaaaggt
     5221 cgccaacacg cagaccgcga cgtgccactt tatcttcata agcaggcccg ataccacgac
     5281 cggtggtgcc gatcgctttc gcgccacgcg ctttctcacg cgcgttatcc agcgcaacgt
     5341 gataatcaag gatcagcgga catgcttcag acagcagcag acgctcacga acggggatgc
     5401 cacggtcttc cagttctttc atctctttca tcagcgcggc cggagacagc acaacaccgt
     5461 taccgatgat gctggttaca ttctcgcgga gaatacctga tggaataaga tggagaacgg
     5521 ttttttcacc gttgattacg agagtatggc ctgcgttgtg accgccctgg tagcgtacaa
     5581 catatttagc ccgttcagtc agaagatcga cgatcttacc tttaccttcg tcaccccatt
     5641 gggtgcccag tacgacgacg ttgttaccca tttttcaaaa tcaccgtttg cttaaaaatg
     5701 gattctacca tcgctttttc agagttacag cactttttgc actcaaaaat gacctcgatc
     5761 agtctgtttt ttgttcagcc aatcgttttc ctcaacatgt agtagaccac aacgcccgca
     5821 accacaagtc caccgccaaa acgacgtaaa atattatcgg gcaaattggt catcgcagag
     5881 atcatcttct tccatgcctt cgggtaaagc atcggcccta aaccttccag taccaaaacc
     5941 agggcaagcg ccagccagat tgtcgaattc attcattatc cttatagaaa aagaaaacca
     6001 ccgacatccc tgaggatgcg gtggctttat tgacctgtac cgcagtcgtt atattaacgc
     6061 gttgcggaag tcggcgtctt catgtagcgg aagaaatcgc tatccgggct catgaccatc
     6121 acgtcctgat tgccagagaa gctgttctca taagcacgca ggctacggat gaatgcgtag
     6181 aagtccggat ctttactgaa tgcatcagca aacagtttgg ctgcttcggc atcgccttca
     6241 ccacgcatga tgcggccctg acgctcagct tctgccagcg ttctggtcac ttcatagtcg
     6301 gcagtcgcgc gcagtttttc cgcttcttcc tgaccttgtg aacggtgacg acgcgctacc
     6361 gcttcacgct cggcgcgcat acggttgtag atcgcttcag acacttcggt cggcaggttg
     6421 atctgcttga tacgcacatc gacaacttca atacccagcg ccgccatact gttcgggttg
     6481 atgaccggaa ctttgccctt cgtctcagcc gttacgcgct ctgccgcttc ggcaatggcg
     6541 ttatctgctg ccggggtagt aacttcatct tctgtacccg cagaaccgga gttcagcgcg
     6601 tcacgtactt cgagggtcag acgaccacgg gaatcagtga cgatatcttt cacgtccagg
     6661 cgaccaattt cagaacgcag acggtcagag aacttacgtt tcaacagcac ttccgcttgc
     6721 gaaatgtcgc caccacccgt tgccaggtag taacggctga aatcgctgat gcgccatttg
     6781 atgtaagagt cgacgatcag gtctttcttc tctttggtca caaagcggtc ggcctggttg
     6841 tccatggtct gaatacgtgc gtcgagcatt ttcaccgttt caatgaacgg tatcttgaaa
     6901 tgcagacccg gctcataaac cagaggtttg ttgtcatcgt cacgcagtac cttaccaaaa
     6961 cgcagcgtaa taccgcgctc accttctttg acgacaaaga cagacatgta aagcactacc
     7021 agcacgatga tgataatcgc gataactgac ttacgcatcg ttattccccc tgacgctggt
     7081 agtcgttacg ctgcgcgttg gcgcggcgtt ggtccataat atcgccctga ctggtggacg
     7141 acgtgttgct tgctccactg gttgtggagg aagaggctgg cggcaaacgc agcagattgc
     7201 tggcaccgtt atcgctcttc gccgcagggg cgttaccacc tttcagcatc tggtctaacg
     7261 gcagaaccat caggttgcca cctttatcgt taaccagcac tttgcgggtg ttacccaaca
     7321 ctttttccat cgtctcgata tacagacgct cgcgagtaat ttccggcgcg gctttatatt
     7381 ccggcagaag tttagcaaag cgcgccactt caccctgagc ttccaggatg gtctgggcct
     7441 tgtacgcacg cgcctcttcg aggatacgtt gcgcctgacc gttcgcacgc ggctgaactt
     7501 cgttggtata cgcttctgct tcacgaatgt attgctgttc gttttcacgc gcggcaatcg
     7561 catcgtcaaa cgccgctttt acttcttccg gcggacgagc agcctggaag ttgacgtcca
     7621 gcagagtgat gcccatgtca tacggacgaa tcgtctcttc cagttcgcgc tgggtatcgc
     7681 tacgaatcac ggtacgacct tccgtcagaa tgcggtccat ggtgtattta ccgataaccc
     7741 cgcgcagggc gctgtcggta gcctgacgca ggctgtcatc cgggctggtc acgctataca
     7801 gatatttttc cggataggta acgcggtact gcacgttcat ctcaacgcgc actacgttct
     7861 cgtccgacgt cagcatcaca ccggaagcgg ccagttcacg cacggcttcc acgtttaccg
     7921 gtttgacttc gtcgataaac gtcggtttcc agttcagacc cggctcaacc agatggctga
     7981 atttaccaaa gcgtgttacc acgccgcgtt cggcttcttt aatggtatag aaaccactgg
     8041 ccgcccagat aatgacaatc gctgccgctg cgatggtaac gacacgaccg ccaagctgcg
     8101 ggcgcgggcc ttgcgatgaa ctgccaccgc cagatccggt gcctttaccg ccgcccagac
     8161 caccgagctt tttgctcagt ttgcggaaga tatcatctaa atcaggtggc ccttgatcgc
     8221 gaccgccttt gtttccattt ccctcagagt tgccgccagg tttgctgctt ccccacgggt
     8281 cgcggtcttg tccgttatta ccgggctgat tccacgccat gtttgtgctc catatttgtt
     8341 atgcggtgat ccctgttgtc tttcgacgcc agaggatatg actccacgct tcagacgcta
     8401 cgccgttaga tcaggtaatc gatcaacgcc ggttcttgtt tacagaggcg acgccagtca
     8461 acgatcggca tacgaacttg cagacttacg ctgccgtcct cctccatcca ctctttttct
     8521 attgcctgaa gctgataaaa acgacttctc agacgccctt cctgcggtgg cagacgcaat
     8581 gtatgctgcg ccacctcgcc ggaaagccgc tccgtcaaag cctgaaaaag ctgtggtatc
     8641 cccgctccgg tctgtgcgga aagccagaca cggatcggtt tgttctcttc gtcccgatca
     8701 atacgcggtt cgaaatcttc cagcatatcg atcttgttca tcaccagcag ggttgggatc
     8761 tcgtgagcgt cgatctcttc aagaaccgta ttcaccgctt cgatgttttc ttgtacacgc
     8821 acatccgccg catcaatgac gtgcagcagt aatgtggctt gccgcgtctc ttgtaacgtg
     8881 gctttaaatg ccgccaccag atcgtgcggc agatggcgaa taaaccctac ggtatctgca
     8941 agtacggttt caccgacatc cgcgacgtca atacgccgca acgtcgggtc gagggtggca
     9001 aacaactggt ctgccgcgta gacccgcgct tcggtgatgc gattgaaaag ggtagattta
     9061 ccggcgttgg tatatcccac cagcgaaaca gtaggaacgt cggctttgat acgcgattgc
     9121 cgcccctgct cacgctgctt ttcaactctt tccaggcgcg actgtatctg cacgatgcga
     9181 ttacgcaaca aacgacggtc ggtttcgagc tgggtttcac ccggaccacg caaacctatc
     9241 ccgcctttct gtctttcaag gtgggtccag ccacgcacca ggcgcgtagc cagatggcgc
     9301 agctgcgcca gctcaacctg caacttaccc tcatgggtac gcgcacgttg ggcgaaaata
     9361 tctaaaataa ggccggtgcg gtcgatgaca cgacactcgc acaaacgctc caggttacgc
     9421 tcttgcgccg ggctcagggc atggtcaaaa agaacgaccg aagcacccgt cgctttgaca
     9481 gcttccgcaa tttcaactgc tttaccttca cctacaaaat actttgggtg cggcgcttta
     9541 cggctaccgg taatcacctg caatgcttcg acaccggcgg aagaaaccag agattcaaac
     9601 tcctggaggt cttccatatc tttgtctttc gtaaaataga tgtgtaccag taccgcctgc
     9661 tcaccagcat cataacggtc aaacaagcgt ataaccctct aaatagatca gcggggaacg
     9721 caggatcgct ggctccccgt gtaaaaaaaa cagcccgaaa ccttattcgg tttcttcgct
     9781 gtcctgttgc gcggaagtat tctgcgcgct gctaccatga tggtagttac tgctggtacc
     9841 gccaccggcg ttgttactgt gatgagaaac cggacgagac gggacaacag tagaaatcgc
     9901 gtgcttgtaa accatctggc tgaccgtgtt tttcaacagg atcacgaact gatcaaaaga
     9961 ctcgatttgc ccttgcagct taataccatt caccaaataa atagaaactg gaacacgttc
    10021 ccgacgcagt gcgttcagga acggatcttg taaagattgc cccttagcca ttctctcttt
    10081 tccttatatg cttatttgta ctttgaacct ttcgattctg aaaaaattgc gcacgatacg
    10141 tctcaattgt acacattcag cctgcgatag caccaacaac ctgtaatact tcgtcatgcg
    10201 cctgttctgg tttttcactg tcaagccagt gaaccccttc ccaaccacgc agccaggtta
    10261 tctgccgctt cgccaactgt ctcgtggcgc aaacacctcg ataaaccatt tcatcgtatg
    10321 agatttcgcc ttcaaggtaa gaccacatct ggcgataacc cacgcaacga atggaaggca
    10381 agtccgtatg caaatctcct cgggcaaaaa gcgcccggac ttctgcttca aaacctgaag
    10441 ccaacatctg atgaaaacgc tgctcaatgc gttgatggag cagttcacgg ctcgccgggg
    10501 cgatggcgaa ctgatgcacc tgatacggta gagcgtctcc tgacgtttgc gtcagttccg
    10561 ttaaagtttt acccgaaatg aaaaaaactt ccagtgcccg ggaaagcctt tgtggatcat
    10621 ttggatgaat ccttgctgcc gcaaccggat ctacctcctg aagttgacga tgcaatgact
    10681 cccaaccttg ctctgccgcc tgttgctcaa ttctggcccg cacttccggg tctgccgacg
    10741 gtagcggcga caacccttcc agcaatgcct tgaaatacaa catcgtaccg cccactaaca
    10801 gtgggatccg ccccgccgcg gtgatatcgg ccatttccgc cagcgcatcg cggcgaaaat
    10861 cagcagccga gtaagcctgc gacggatcgc gaatatccag caatcggtgc ggcgcggcga
    10921 gtaactcttc agcgttcggc ttcgccgtcc cgatatccat ccctttgtaa ataagggcag
    10981 aatcaacgct tatcaactct actggtaaaa ttttacgcag ctcaatggct aacgccgttt
    11041 taccggaggc cgtcggcccc atcaaaaaaa tcgccttagg caggctcgcc ttactgatat
    11101 cactcatctt tcagggcttt tatcgccgga tgtaaatcaa cagattgtaa cagaccaccc
    11161 ggcggcgttt tcacaagttg cggacataac cgttccacgt ccgccagcag ggttatggcc
    11221 tgtgccattg accactgcgc atgttcgctc atcagatttc gtgcaatcca ctgcgcaata
    11281 ttgccaggtt cgaatacgga ctgcttcgcc aggtagccta tcagttcagg aatcaagatt
    11341 tgtaaatttt gttggcgtaa gggtaaaggc acggccctga tggtcacatg ctgtgcatct
    11401 gactggaaat caatacccaa ttccgccagg gcagactgcg ctttttctaa tgccgatttt
    11461 tcttcgccag aaacttttag ccgcaacgga ataagcagcg gctgagcgca aactggcgct
    11521 tcacccgggg tcagttgtac ctgacgcagc caacgttctg ccactggcaa ggctaaaagt
    11581 gaaatgttgc cgtcgcgctc cagcaacgca cagtcggaat ggacgatagt cagtacccga
    11641 ccaaaactct gactgttcgc cgcaagtgca ggttcctgcg gttccggcgc ttttaatttt
    11701 tgcatcggcg cgggcgtttg caaaagctgg cgatacactt caccttgctg tttctggtag
    11761 cctggctgcg cattcggcca aggggcagcc ggacgactgc ctgatgctgg cgcaggagtg
    11821 tagcgcggag ctaccggctc acgagctgcc ggttctgcaa agtgattgcg ccccgccgcc
    11881 acgcggtttt ccggaatgga acgcggtgca ggttggggtt catcgtccag cggtagcggc
    11941 gtttccagtt gctgttgtag cacgctcagc acgccctgat agataaaatc atgcaccaga
    12001 cgcgactgat ggaaacgcac ttcgtgtttg gcggggtgca cgttgacgtc cacctgatgc
    12061 ggatcgatct ccagatacag cacaaatgcc ggttgctgat cggcccccag tttgtcttcg
    12121 caggcctggc ggatcgcgtg attgatcagg cgatcgcgca tcatgcgacc gttcacgtag
    12181 caatactgaa tttctgccag tgcgggcgtg gtgtgatttg gatcggccac ccagccgcgt
    12241 agcgtgagat cgccgtgttg ccattcaatc gccagcgctt gttcaagaaa agcggtgccg
    12301 caaatcgcgc ctaagcgccg ttctttttgc ccgccttccg gcactgcgcg gtactgacgc
    12361 acaattttac cgttatgcga caggttgatc gtgacgtcga aacgcgccag cgcaatgcgg
    12421 cggatgatct catcaatgtg gttaaattcg gttttctcgg tgcgcaggaa tttgcgccgc
    12481 gccggggtgt tgtagaacag atccagcacc tccagcgtcg tccccacagg atgcgccgcc
    12541 ggttttaccg tcacgttcat atcgcgccct tcggcatagg cctgccaggc ttcctgctgt
    12601 tctgcggtgc gtgaagtgag cgtcaggcgg gaaaccgaac tgatactcgc cagcgcctca
    12661 ccgcgaaagc ccaggctgat aatggcttcg agatcgtcca gagaggcgat tttactggtg
    12721 gcatgacgag ccagcgccag cgccagctca tcttttttga taccgcagcc gttatcacga
    12781 atgcggataa gtttcgcccc accgcgttcg atatcaatat cgatacgcgt cgcacctgca
    12841 tcgaggctgt tttccaccag ttctttgact accgacgcag gtcgctcgac cacctcacct
    12901 gcggcaatct ggttcgccag ttgtggcggt aagacctgaa ttggcatcaa tcgtccttag
    12961 tttggcagcg tgcgatctgg cgtcgtcacc gtactggccg tttgtgccgt tgcaccctgc
    13021 ggcgcagatt gcatcggatg cgcaaggaaa taattgcgca ggcctttgta aatggcttct
    13081 gccagctgtt gttggtaatc gtcgctcgcc agcaaacgtt cttcgctgtt gttgctgata
    13141 aaaccggttt cgaccagtac tgacgggata tccggcgaac gcagaacgcc gagactcgcg
    13201 tgttctggtc ggcgtttatg tatttcgcca atgcgttgca actgactgat catactggtc
    13261 gctacatcat accctacccg ctgggaatga ccgaactgta aatccagcac cgcctggctt
    13321 aaatagggat cagactgact gttcgccagc acatcgcccg ccccacccag cagctccgaa
    13381 tgtttctcgt gctgctccag ccagctggcc atttcgctgt tggcgcgacg gttagagagc
    13441 acccatacgg aagcgccagt cgcactgcgg ttcggtgcgg catcagcgtg aatcgacacg
    13501 aggaaattag cgttttgctt acgtgccaca tcgctgcgcc ccatcaccga aataaagtaa
    13561 tccccgtcac gggttaaaac gcctttaaac atcggatcgt cattgagcaa agtacgcaat
    13621 ttacgcgcga tggcgatggt gacatttttc tcccgcgtac caccggggcc gatagcgcca
    13681 gggtcctgac cgccgtgtcc ggcatcaata gcgatgataa ttttatcgcc agtgttagcc
    13741 gtcgcgcgcg ctgccggacg cgttaccgta ttactgctga taacacccgt agtgcggtta
    13801 ctttccgttt taaacggatt gcgcgccggt tcgctgacgc gcggtgcgac aaccgcaggc
    13861 gtttcaacgc gtttcgcaac cacgggcggc ggaggaggcg gtggcggcac atcggcgtta
    13921 atcgtaaaga cgacagtgta attgctgcca ttctgccgct tcaccgcttc ggttttacca
    13981 ttttcggtaa gatcgaccac cagccgtagc gtttgtgcat ctttaggcgt tccagagcga
    14041 atcgccttca ccagattatt gccgctgaac aacaacggca gtccctgaat cacgcccgtt
    14101 tgtttgatat cgagcgccac ggtgcgtttg ctttgatggc taaacgcata atcaggatcg
    14161 ccaataaaac tcaacgttat ccgcgcctgt tgattaccgt tagaaacctg aatatcagag
    14221 agcgtcgcgg cacccaccgg cgtgcacagc agcagcagcg tcgctaccaa ccaatttctg
    14281 atgcgataca tcatcccgcc acctttcaaa ggttaaccgg ctaaacgcgc cagcaacaat
    14341 tcacccgcag aggaaaccgc actcacgcgc gcctcacggc cttgtgcctg ataatcaatg
    14401 tgtatttcga catccgggtc aggaagaaca cctgtacctt gttgtggcca ctccaccagg
    14461 cagatggcat cgttggcaaa ataatcgcgg atccccataa actccagctc ctcgggatcg
    14521 gcaaggcggt acaaatcaaa gtgataaacc attaaattgt cgagcgtata gggttcgacc
    14581 agcgtataag tggggctttt gacattaccc tgatgaccca gagcttgtaa aaagccccgg
    14641 ctaaaggtgg tcttacctgc gcctaaatcg ccatacagat agattacggt tgcgccatcg
    14701 caggctttcg ctacccgctc gcccaggtct aatgttgcct gctcatcagg gagcggaatt
    14761 actcgattca tcatggtttt tatcagtcac ttccgggtta acaatacgct gtagcgtgga
    14821 aaagagatcg gttgccagca tcccgcgcgt tccaaaacgc gccgccagta cgtcagctgc
    14881 cgcaccgtgc gcgacacagc ccgcacaggc ggcatcatac ggcgacagtt tttgcccaag
    14941 caatgcgcca ataataccag agagcacatc gcccatgccg ccgctcgcca tgcctgcatt
    15001 tccggcatca ataatgccta aagcgtcagg atgggcggcg accacggttc cggcaccttt
    15061 cagcaccgct acgccgccat aacgttgtac cagacgtttg gcgcaatgta agcggtcact
    15121 ttcaatttca gcgacggaac agcctaacaa ccgtgcggcc tcgccaggat gcggcgtgat
    15181 cacgcgattg tgacgcttat cgggattgat tgccagcagg ttcaatgcat cggcatccca
    15241 caacatcggt ttgcgaaaat tctcaacttt ttgcagtgct tttttccccc actcttgctg
    15301 gcccagaccg ggaccaatca ccaccacatc ggcccattcc aggctttcgg taagagagtc
    15361 catcgtcagt tcatgcacca tcaattccgg tcgtgcagtc agcagcggcg caatgttttc
    15421 actgcgggtc agtactcgga ctaaaccagc accagcacgc agcgccgctt cccccgtcat
    15481 acgaatagcc cccgccgtgc cgtgatcgcc accgataatt accagccgcc cgtgatcgcc
    15541 tttatgcgaa gtcgggcgac gcggttttag ccagtgagaa agttgttctg ctgaaaaccg
    15601 ctgaattttc gtctcctgac ctgccagcca actatccagc cccagtgagt caaaatgcag
    15661 ttgtccggta acatcccgcg cttttccagt gagcaagcct agtttcagcg caataaaagt
    15721 gatggtgtga tcggcgttga tcaccgcgcc tggcgtagcg ccagtttcag ccagcaggcc
    15781 ggaagggata tcaaccgcca caatcggcgc aggatgggaa ttagcgtggt cgattaactg
    15841 gctaatggat tcgcggggcg cttgccgcaa accggtaccg agcagcgcat caacaatcag
    15901 atctaccgat tcgggccaga caatattcga agcatggatc tcgccacccg cgtttaacca
    15961 tgcttcgcgt gccagcgcgg cctcttccgg caacggtttg tcgctctcct gggcaaacaa
    16021 cgtgacctca atgccgaccg ctttggccag tcgcgcgacc acgtagccat cgccgccgtt
    16081 attaccatga ccgcacagca ccagccagtg gcgggcgtca ggatacgccg aacgacacac
    16141 ctggaatgcg gcctcgccag cgcgaagcat cagctcatag agtgtgagcc ccagcacatc
    16201 tgccgcctcg cgttctccgc ggcggatatc gtcggcgtac cagacggtgt gtggtatact
    16261 tacggggttt ttcttcattg tatggtccgt catgtcagag cccctcgatc tcaatcagtt
    16321 agcgcaaaaa attaaacagt gggggctgga actgggcttt cagcaggtag gtattaccga
    16381 taccgatctc agcaagtccg agcccaaact gcaagcatgg ctggacaaac aataccacgg
    16441 cgaaatggac tggatggcac gtcacggtat gctgcgcgct cgccctcatg agttattgcc
    16501 cggtacgctg cgcgtgatca gcgtgcggat gaattacctt cctgctaacg ccgcatttgc
    16561 cagcacgctg aaaaacccca aactcggcta tgttagccgt tatgcgctgg gccgtgacta
    16621 tcacaaactt ctgcgcaacc gactcaaaaa gctgggcgag atgattcagc aacattgtgt
    16681 ttcgctgaat tttagaccgt ttgtcgattc tgcgcctatt ctcgagcgcc cgttagctga
    16741 aaaagctggg ctcggctgga caggtaagca ctcacttatc ctcaatcgcg aggccggttc
    16801 gttcttcttt ttaggcgaat tgctggtcga tattccgctg cccgtggatc aaccagtcga
    16861 ggaaggatgc ggtaaatgcg tggcctgtat gacgatttgc ccgaccggtg ccatcgtcga
    16921 gccatatacc gtcgatgctc gccgctgtat ctcttatctc accatcgaac ttgaaggggc
    16981 gatcccggaa gagttgcgac cgttaatggg aaaccgtatt tacggttgcg atgactgcca
    17041 gcctatctgc ccgtggaatc gctattcaca actcaccaca gaagaggatt tcagcccgcg
    17101 taagccgcta cacgcaccgg aactcattga gttattcgcc tggagcgaag agaagttttt
    17161 aaaagtcacg gaaggatcgg cgattcgtcg aattggtcac ctgcgttggc tgcgtaatat
    17221 cgccgtagca ttaggcaatg ccccttggga tgaaacgatt ttgacagcgc tggaaagtcg
    17281 taaaggtgag cacccacttc tcgatgagca catagcgtgg gcgattgcgc agcaaataga
    17341 gagacgaaat gcgtgcatag tcgaagtgca attgccgaaa aaacagcgtc tggttcgggt
    17401 gattgaaaaa gggttaccgc gtgacgcctg attcatccac agcttgtgaa taaaaataaa
    17461 aacgcattgc aattcaagac gaataaaatc gtcaagtgat cgactcgaca aaacggaatc
    17521 ttaattttta acttatctat caataggtta aaaaaatact attcaccgtg cgaagtttct
    17581 ttgaaattaa gattacagcc tgaggctgtg gataactctg tttacaagag tttgtcagag
    17641 acaacgaaaa ccagcaataa cgcatcgctg tggatatttt attgagagaa ga
//
LOCUS       DADLFA010000077        17503 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000077 DADLFA010000000
VERSION     DADLFA010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17503
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.077"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..744
                     /locus_tag="JQH65_002192"
     CDS             <1..744
                     /locus_tag="JQH65_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HAY6139590.1"
                     /translation="SSTDKPAKLKFHQYARATLLSNRGRDHNCDGRRTVEIHKLDLSD
                     WQAFNKLATKCNAYDGVTMNGDNSFGWNHEATLDNIHAQKYNKAYAGARLTAELKYLL
                     QDVESFEPNSKYTIHEVVLGPGYGTPDYTGQTIGYVVTLPAQMPNCWSSELPTIDLYI
                     DQLHTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNSAAIYSGLGFERVPQDRD
                     IRSDRMHLTPANHPDLWTQENGEWIYLRN"
     gene            complement(815..1264)
                     /gene="rplI"
                     /locus_tag="JQH65_002193"
     CDS             complement(815..1264)
                     /gene="rplI"
                     /locus_tag="JQH65_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAY6139591.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(1306..1533)
                     /gene="rpsR"
                     /locus_tag="JQH65_002194"
     CDS             complement(1306..1533)
                     /gene="rpsR"
                     /locus_tag="JQH65_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAY6139592.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(1538..1852)
                     /gene="priB"
                     /locus_tag="JQH65_002195"
     CDS             complement(1538..1852)
                     /gene="priB"
                     /locus_tag="JQH65_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HAY6139593.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(1859..2254)
                     /gene="rpsF"
                     /locus_tag="JQH65_002196"
     CDS             complement(1859..2254)
                     /gene="rpsF"
                     /locus_tag="JQH65_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAY6139594.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            2581..2856
                     /locus_tag="JQH65_002197"
     CDS             2581..2856
                     /locus_tag="JQH65_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY6139595.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(2931..3482)
                     /locus_tag="JQH65_002198"
     CDS             complement(2931..3482)
                     /locus_tag="JQH65_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1440 domain-containing protein"
                     /protein_id="HAY6139596.1"
                     /translation="MAINGLFVRTDPQRRRYGVALLVGVLSGIISAFVKWGAEVPLPP
                     RTFSGGRDEFNPPYIFLRDYLGIDPTQTVYTFSEHIINPVMVTHIIFSLVFAIGYCIV
                     AEIFPKVKLWQGILAGLIVTVAVHGIFCPALNLTPPLTQLPFDEYASEILGHIFWFWI
                     IEIMRRDLRNRITHEPDPEVAIR"
     gene            complement(3579..4265)
                     /locus_tag="JQH65_002199"
     CDS             complement(3579..4265)
                     /locus_tag="JQH65_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAY6139597.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGKYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(4265..5119)
                     /locus_tag="JQH65_002200"
     CDS             complement(4265..5119)
                     /locus_tag="JQH65_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HAY6139598.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARDWVKARMA
                     KAGMVEAA"
     gene            complement(5129..5779)
                     /gene="ulaD"
                     /locus_tag="JQH65_002201"
     CDS             complement(5129..5779)
                     /gene="ulaD"
                     /locus_tag="JQH65_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAY6139599.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(5793..6257)
                     /gene="ulaC"
                     /locus_tag="JQH65_002202"
     CDS             complement(5793..6257)
                     /gene="ulaC"
                     /locus_tag="JQH65_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HAY6139600.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(6267..6572)
                     /gene="ulaB"
                     /locus_tag="JQH65_002203"
     CDS             complement(6267..6572)
                     /gene="ulaB"
                     /locus_tag="JQH65_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HAY6139601.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            complement(6588..8036)
                     /gene="ulaA"
                     /locus_tag="JQH65_002204"
     CDS             complement(6588..8036)
                     /gene="ulaA"
                     /locus_tag="JQH65_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005009981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAY6139602.1"
                     /translation="MSAQHSGCAEYPVTSSSGDRLYNIFTVFFNQVMTNAPLLLGIVT
                     CLGYILLRKSVSVIIKGTIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISD
                     TYASMMATIDRMGDAYSWVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGL
                     IAVTLFIFGYSMWTTIICTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASW
                     IAYKVAPFLGKKEESVEDLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAM
                     AGKVNWTVYILQTGFSFAVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAA
                     IYSFAPNAVVWGFMWGTIGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFG
                     GWRAALKICLVMGMIEIFGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMA
                     VIIVIALAYMFFAVRALRAEEDAEKQLAEQSA"
     gene            8340..9404
                     /gene="ulaG"
                     /locus_tag="JQH65_002205"
     CDS             8340..9404
                     /gene="ulaG"
                     /locus_tag="JQH65_002205"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAY6139603.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTPPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQAAPQEIRVQWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            9512..10267
                     /gene="ulaR"
                     /locus_tag="JQH65_002206"
     CDS             9512..10267
                     /gene="ulaR"
                     /locus_tag="JQH65_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HAY6139604.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(10264..11013)
                     /gene="yjfP"
                     /locus_tag="JQH65_002207"
     CDS             complement(10264..11013)
                     /gene="yjfP"
                     /locus_tag="JQH65_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAY6139605.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSSDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            11195..11524
                     /gene="bsmA"
                     /locus_tag="JQH65_002208"
     CDS             11195..11524
                     /gene="bsmA"
                     /locus_tag="JQH65_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HAY6139606.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTNHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            11673..11948
                     /gene="yjfN"
                     /locus_tag="JQH65_002209"
     CDS             11673..11948
                     /gene="yjfN"
                     /locus_tag="JQH65_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HAY6139607.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(12065..13690)
                     /locus_tag="JQH65_002210"
     CDS             complement(12065..13690)
                     /locus_tag="JQH65_002210"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HAY6139608.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSLLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMCHVLSRMALQLEGQTALLFRLARAWDRCADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(13774..14832)
                     /locus_tag="JQH65_002211"
     CDS             complement(13774..14832)
                     /locus_tag="JQH65_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HAY6139609.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNAIQERLISRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQ
                     QAGQESRFIYIEDLGLGVGGVLTDLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQW
                     VEPLWKSILSNKGLMPLLWRFFPGHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGN
                     VTIFDGQNNVVDHADGDYADEPMIYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIRED
                     NTLITKDTSRFVPHYIAG"
     gene            complement(14835..15473)
                     /locus_tag="JQH65_002212"
     CDS             complement(14835..15473)
                     /locus_tag="JQH65_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HAY6139610.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWLNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(15483..15881)
                     /locus_tag="JQH65_002213"
     CDS             complement(15483..15881)
                     /locus_tag="JQH65_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HAY6139611.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAVSLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(15899..16558)
                     /locus_tag="JQH65_002214"
     CDS             complement(15899..16558)
                     /locus_tag="JQH65_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HAY6139612.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSRIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKTMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(16609..17307)
                     /locus_tag="JQH65_002215"
     CDS             complement(16609..17307)
                     /locus_tag="JQH65_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HAY6139613.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSR
                     AQDVLARLQRQQGE"
     gene            complement(17380..>17503)
                     /locus_tag="JQH65_002216"
     CDS             complement(17380..>17503)
                     /locus_tag="JQH65_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HAY6139614.1"
                     /translation="QKMMPLSSVGISSVQQEEYYIVFGALSLKSSLEDILLEIT"
BASE COUNT         4467 a         4676 c         4186 g         4174 t
ORIGIN      
        1 tcgtccaccg acaaaccagc aaaattaaaa tttcatcaat acgcacgcgc tacattactg
       61 tcaaatcgtg gaagagatca taattgcgat ggcagacgta ctgttgagat ccacaaacta
      121 gatctctctg actggcaagc attcaataag ttagcaacga agtgtaatgc ttacgatgga
      181 gtaacaatga atggtgataa ttcttttgga tggaaccatg aagctacatt agataatatt
      241 cacgcccaga aatacaataa agcttacgcg ggtgcgaggt taaccgcaga actgaaatat
      301 ttattgcaag atgtcgaatc cttcgaaccc aacagtaaat acacaataca cgaagtggtt
      361 ttgggacctg gttatggtac tccagactat accggccaaa cgataggata cgttgtaacc
      421 cttcctgccc aaatgcctaa ttgctggtca tctgaactac caacaatcga tctttatatc
      481 gaccaacttc acactgttac aggcgtttct aatgccctcg gattcatcat tgctgcattg
      541 ctaaacgcgt attctgattt accccatgat ttgaaaattg gtctgcgttc cttatcaaat
      601 tcagcagcaa tctactcggg tttaggtttc gagcgagtgc cgcaggaccg agacataagg
      661 agtgatcgta tgcatctaac gccagcaaac catcctgact tatggacaca agagaatggt
      721 gaatggatct acttgcgaaa ctaatacctg acgaaaaata gaaagcaaaa cgccgaccaa
      781 tggtcggcgt ttttacgtct cgttgaataa cgaattattc agctactacg tttacgatca
      841 ctttcgcgaa tacttcgctg tgaacctgga agctcacttc gtgttcgcca gtggtacgca
      901 gaacgccgtt cggcagacga acttcgctct tagccacttc aacgccagct gcagttacag
      961 cgtcagcgat gtcgcgagta ccgatggaac cgaacagttt accttcgtcg ccagctttag
     1021 acgcgatggt aacagtttcc agtgcattga ttttctcagc gcgagcatta gcagctgcca
     1081 gaacttcagc cagtttagct tccagttcag cgcgacgtgc ttcgaagaat tcaatgtttt
     1141 tcttggtagc tggaacagct ttaccctgcg gtaccaggaa gttacgagca tagcccgctt
     1201 taacgtttac ctgatcacct aggctaccca ggtttgctac tttatcaagc agaataactt
     1261 gcattacctt atcctctcaa agtcgtatta atggaccgtg accgattact gatggcgatc
     1321 agtgtacggc agcagggaca ggtagcgagc gcgtttgata gcgcgagcca gctgacgctg
     1381 gtattttgca cgggtaccgg tgatacggct tgggacaatc ttaccgcttt cggtgatgta
     1441 gtttttcagc gtagcgatat ctttatagtc gatctcttga acgccttccg cggtgaaacg
     1501 gcagaacttg cgacgacgga aataacgtgc catatggcta gtctccagaa tctatcaatt
     1561 caatctgctc ggcatgcaaa accattttgc tcagtccgtt ctttgccttg tggcatgaaa
     1621 tgaacccctg aacggttatg cgactgccga ccgttatact gtgagtaatg gcttggtttt
     1681 cgtgtccgct aacaataacg ggcatttggc accacgcctg ccggtgaaaa ccggcttcct
     1741 cctgcacaga acgatgctca agcacgaact ggcagtgagg aattcctgat ggactgacct
     1801 ttcgaagggg agtcctgcac acggtgccgg acaacaccag acggttggtc atcagaaatt
     1861 actcttcaga atccccagct tcagcatcat cagcggtttc gtttgcgaaa tcatcgcgac
     1921 gctcacggcg ctcgtctttc gctttaacca tcggagatgc ttcggtaaca gcgtgcttgg
     1981 tacgcataac catgctgcgg ataacggcat cgttgaagcg gaaggtagtt tccagctcat
     2041 cgatcacttc ctgcggagct tcaacgttca tcagaacgta gtgtgctttg tgcagtttgt
     2101 tgatcgggta agccagctga cggcggcccc agtcttccag acggtggatc ttgccttctg
     2161 caccagtgat ggcagcagtg tagcgctcga tcatgcccgg aacctgttcg ctctggtcag
     2221 gatggaccat aaaaacgatt tcgtaatgac gcatcgaatt gctccttacg gattattcag
     2281 cctcctgtct gggtcagccg aatcccgggg aggcaaggaa cgtgttaaag gtcggctgaa
     2341 aaatgacgct ttatagtact tgtatgcccc actaaactca aggccagccg acaaataaat
     2401 ctcatctttc gctcaaaaga actaactcgc tgatttgaag ttcatcacat tccgtcacag
     2461 tgttttttcg aacaactgca tcagaaatgg tcaccatcaa cttctttatc attgattaaa
     2521 attaaatcag aagatcatca cagaggagag cacacaaagt cagccgtcga ggaggtatct
     2581 atgttcagtc gtgttttagc ccttctggct gtgcttttgc taagtgcaaa tacatgggca
     2641 gccattgaaa ttaataacca ccaggccaga aatatggacg acgtacagag cttaggcgtg
     2701 atttatatca atcataattt cgccactgaa agcgaagcac gtcaggcatt aaatgaagag
     2761 acagatgcgc agggcgcaac gtactaccac gtaattctga tgcgggaacc ggggagtaac
     2821 ggcaatatgc acgccagcgc ggatatttat cgctagcacc aggtataacc aacgaaacat
     2881 tgccatagtt tgctttgccc ccttcgcagg gggctttttt atacggttat tcaccggatt
     2941 gcgacttccg gatctggctc atgagtaatg cgattacgta agtcgcggcg cataatttct
     3001 attatccaga accagaaaat atgccccagg atttcagagg catattcgtc aaaaggcaat
     3061 tgagtgagcg gcggcgttaa attcagcgcc gggcaaaata taccgtggac tgccactgtc
     3121 acaataagcc ctgctaatat cccctgccat aatttcactt tcgggaaaat ttccgcgaca
     3181 atacaatacc ctatcgcaaa aaccagtgag aagatgatat gcgtcaccat caccgggttg
     3241 ataatatgtt ccgagaatgt atacacagtt tgggttgggt cgatacctaa ataatcccgt
     3301 agaaagatat acggcgggtt aaattcatcg cgaccaccgg aaaatgtgcg cggtggcaag
     3361 gggacttccg ctccccattt aacaaatgct gaaataattc ctgaaagtac acccacaagt
     3421 aacgcaacgc catagcggcg acgttgcgga tccgttctga caaacaatcc attgatagcc
     3481 ataaaaacac ctgttgttta tacgattttt taactgaagc ggccccggaa tcagaccaat
     3541 tacatcccgc aggaggtaat aattccgggg cgcactgctt acttctgccc gtaataggcg
     3601 ttaggaccgt gcttacgcat gaagtgttta ttcatgagga agctgtcgat gtgattcagt
     3661 tgtgggttaa tactgcgcgc aatccacgcc attttcgcca cttcttccat caccaccgcg
     3721 ttatgcaccg cgtcgtgggc atctttcccc caggcgaacg gcccgtgctg atacaccaca
     3781 attcccggcg tatgcagcgg ctcggcgtca cccagcgttt cgataatcac tttgccggtg
     3841 ttcagttcat actcgccctg cacctcttct tcgcttaatc cacgcgtaca cgggatgtcg
     3901 ccaaagaagt agtcggcgtg cgtggtgcct aacgccggga tcgccagccc cgcctgcgcc
     3961 catgcggtgg catgagtgga atgggtatgg acaatgccgc cgagcgacgg gtaacgacgg
     4021 tagagttcaa gatgcgtcgc ggtgtcagaa gacgggcgat acttcccttc taccaccttg
     4081 ccgctcatat cgaccaccac catatcgtct gctttcatgg tttcgtaggc aacgccgctg
     4141 ggcttgatca ccaccagccc gcgttcgcgg tcgatagcgc tgacgttgcc ccaggtaaag
     4201 gtcaccagcc catagcgcgg cagatccatg ttggcttcaa acacctgctg ttttagcttt
     4261 tgcattatgc cgcctccacc atgcctgctt tggccatgcg cgctttcacc caatcacgcg
     4321 ctttcaccac ttctgccgcc gggtcttccg ccgtttcgct ccacatctca atcaggtacg
     4381 gcccgcaata gccactctgt ttgagcgttt cgaagcaacg ttcgaaatcc accacacctt
     4441 cgccaaatgg cacgtttttg aagacgcccg gtttggtgtc tttcacatgc accgcgacga
     4501 tatgcccgat tccggcctgc aactccatct gcacatcgtt gtcccacgcc gacaggttgc
     4561 cgatatctgg gtagagctgg aaccacgggt tgttgagata gtgcgcgtaa cccagcgcct
     4621 tgctgatgga gttcatcaac ggataatcca tgatctccat cgccagcgtc acctgcgcgc
     4681 ggctcgccat ctcaacgctc tctttcaggc cgtcacggaa acgacgacgc gtttcgttat
     4741 tggcttcctg atagtaaacg tcatagcccg ccagctggat cacgcgaatg ccaacgtcct
     4801 gggcgaactg gatggcttta cgcataatct ccagcccctg cgcccgcacc gcgtcatctt
     4861 cgctgcccaa cgggaaacga cgatgggcag aaaggcacat ggacggcaca cgcacgccgg
     4921 tttcaacaat cgcattcacc agcgccagac gctgctcgcg gctccagtcg aggcgcgaca
     4981 ggcgctcgtc agtttcatct accgacatct cgacaaaatc gaagcccaac gtttttgcca
     5041 gtcgcaggcg ttccagccag cactccccgg cggggagcgc tttttcatag atgccaagcg
     5101 ggatttgttt ggacaacata tccgctcctt agccccacag ttcggcgatg gaacgtttga
     5161 actgacgcgc ggcttcaacc ggagaagcgg catcacggat gctgcgaccg gcgataaaca
     5221 catgaattgg aatgcctttg aacagcggca gatcttccag cgccaggcca ccggtgacgg
     5281 tgactttgaa gcccatatcg gaaagacgtt tgatcgcagt gatatcggct tcaccccacg
     5341 ccacgccagc ggcctgcgcg tcacggctgc ggtgataaac cacctgctga atacctgcgt
     5401 cgcgccactg ttgcgcctgt tcccaggtcc agtaaccggt cagttcgatc tgcacgtcgc
     5461 cgttaaactc ttttgctacg tccagcgcac ctttggcggt gttgatatcc gcacaacaaa
     5521 tcacggtcac ccagtcagcg ttggcttcga agcacatacg agaaagaatt ttgcccgcat
     5581 cggcaatttt ggcgtctgcc agcacgattt tgtgcgggta gagcgctttc agatcgcgaa
     5641 ccgcacgcac gccttcgccc acgcacagaa tggtacccac ttcgataatg tcgacttctt
     5701 cggcaatcag gcgagtggtt tcgtaggcgc tatccatagt ctggttgtcc agcgcgactt
     5761 gcaacatcgg taatgacatc ttcaattcct tcttaagctg ccgcgttggt gcggtcaatg
     5821 agatccagta cttcctgctc ggtacggcag gcacgtaaac ggtcgaaatt ttcttcatct
     5881 tcaaacaggt tgacgatctg catgatgccc acttcctggt gcgtgttggc atcgaccgcc
     5941 gccatggtga tgaggatatc caccgggtcg ttatcttcgt ggttgaactc cagcggcttt
     6001 ttcagcgtca ccagcgcaaa accggttttc ttaacgccct cttccgggcg accgtgcggc
     6061 attgccaggc ctggtgcgag cacaaagtac ggaccgtgct gttcaaccgc atccagaatc
     6121 gcctggtagt aacgcggctc gacaacatcc gccgccacca gcaggtcaac gccgattttt
     6181 actgcgtcct gccatgtctc agcttcagcc tgcaggcgga tggatttatt ctccgccagc
     6241 gaatcacgta atttcatggc gcgtccttac ttcacatcct gcgggaaatg cgctttgatc
     6301 acttccagca gtttcgggcc aaagtcggca ggagagagca tgttgcgcac gccaaccacg
     6361 tatttgttgc cggtcacggt gatttcgccc gcaatgtgcg tagaagcgat gatgatatcc
     6421 gcgccgctca actcgctttt gtattcgcca accgcgcagc tatttaccgt gtggtcaatg
     6481 tttgattggg ttaaaaactg gtccactttc atcttcatga tcatggaact gccttgtccg
     6541 ttgccacaca cagccagaat acgtacggtc ataatcaaaa ctccttatta agcagactgt
     6601 tctgccagtt gtttttctgc atcttcttct gcgcgcagtg cgcggacagc gaagaacata
     6661 taagccagtg caattacaat gatgacggcc ataaaggcga taccgatgga gaagaagccc
     6721 tgcatcatcg gcggtgccag aatcgaccag tccgccatgc ccatccaggc gctcataccg
     6781 gtgagtttca ccgcccagac gcagccaaag atttcaatca tccccatcac cagacatatc
     6841 ttcagcgccg cacgccagcc gccgaagtgg ttagcgaaca cgccgatggt ggcgttggag
     6901 aagaacatcg ggataaagcc gggaataatc aggatcgagg aaccgcaggc gaccaggatg
     6961 ccaaccgcaa tcagctgacc gatggtgccc cacataaagc cccaaaccac ggcgttcggc
     7021 gcgaagctat agatagccgc acagtcaatc gccagaaccg caccagggat caggcgctgg
     7081 gagataccgt taaatgcttc agagagttcc gccacaaaca tgcgcacgcc ctgagtgatg
     7141 atgaagatcg ccaccgcgaa ggagaaacca gtttgcagga tgtacaccgt ccagttcact
     7201 ttgcctgcca tcgcctgcac ggtgtcgata ccgaaggaga gtagaatggc accaaagaag
     7261 atggtcatca caatcgccgt ggagacgatg ttgtcgtgga aaatattcag ccagcctggc
     7321 agtttgaggt cttcaacgct ttcctctttt ttgccgagga acggcgcaac tttataggca
     7381 atccatgatg caaactgttg ctggtgaccg atggagaaac cgcagccgtc cgtcacttcc
     7441 tgagtcggct tgtacatcat attggaggta atgccccagt agagcgaaac cagaatcgcc
     7501 gtgcagataa tggttgtcca catggagtag ccgaaaataa acaacgtaac ggcaatcagc
     7561 cccgcctgct ggaacatgat atgaccagtc agcatgatgg tacgaatacc ggtaatgcga
     7621 cgcagcagca cgtaacagat gttcagcgcc agcgctaaca acaccgcgta acctacccag
     7681 ctataggcat cgcccatgcg gtcgatggtt gccatcatcg acgcgtaagt atcggaaatt
     7741 gcgccgttaa tgccgtagac ttcggacatt ttcgccacca ccggtttgaa ggtgctggtg
     7801 aggatgccgg accctgcctg caacaacatg aaaccaatta tggttttaat cgtgccttta
     7861 ataataacgc tgacactttt gcgcagtagg atgtagccca gacaggtcac aatacccagc
     7921 aacaacgggg cattggtcat gacctggtta aaaaacacgg taaagatgtt gtagagacga
     7981 tctcctgaag aagaggtaac cgggtattcc gcacacccgg aatgttgtgc actcactcta
     8041 attttcaaaa gcaatcacaa caagattatt tatgattaaa tgtgacgcga cccgcaaata
     8101 attccatacg gatagagtgg gattcacaca acgaaacaat tatttattta aatttagtta
     8161 aattcattta gttaatttaa ctatacattc cattaaatct ttccacactc aaaatcaaca
     8221 acaacttccc tggcaaaaag tcaaaaaatg gtcattgcct tcctgaaaaa atagtgtcag
     8281 gattaatcaa aaccaatcac atattgattc gaattgaact atcatgaagg taaatggcga
     8341 tgagtaaagt gaaaagtatc acccgagaat cctggatcct gagcactttc ccggagtggg
     8401 gtagctggct gaatgaagaa attgaacaag agcaggtcgc ccctggcaca tttgcgatgt
     8461 ggtggctagg ctgcaccggg atctggttga aatcagaagg tggcaccaac gtttgcgttg
     8521 atttctggtg cggcactggc aaacaaagtc acggtaaccc gttaatgaaa cagggtcacc
     8581 agatgcagcg catggctggc gtgaaaaaac tgcagccaaa cctacgcacc cccccgtttg
     8641 ttcttgatcc gtttgcgatt cgccagatcg acgcggtact ggcgactcac gatcacaacg
     8701 atcatatcga cgttaacgtc gctgctgccg tgatgcagaa ctgtgctgat gacgtaccat
     8761 tcattggacc gaaaacctgc gttgatttgt ggattggctg gggcgtaccg aaagagcgtt
     8821 gcatcgtggt caaaccgggc gatgtggtga aagtgaaaga cattgaaatt catgcgcttg
     8881 atgctttcga ccgtactgca ctgatcaccc tgcctgccga tcaaaaagcg gctggcgtac
     8941 tgccggacgg catggacgat cgcgcggtga actatctgtt caaaacgcct ggcggctccc
     9001 tgtatcacag cggcgactcc cactactcta actattatgc gaagcacggt aacgaacatc
     9061 agatcgacgt ggctttaggt tcatacggcg aaaacccgcg cggtatcacc gacaaaatga
     9121 ccagcgccga tatgctgcgt atgggtgaag ccctgaatgc gaaagtggtt atcccgttcc
     9181 accacgatat ctggtcaaac ttccaggccg ctccgcaaga gatccgcgtg cagtgggaga
     9241 tgaaaaaaga tcgtctgaag tatggcttca agccgtttat ctggcaggtt ggcggcaaat
     9301 ttacctggcc actggataaa gacaacttcg agtaccacta tccgcgcggt ttcgatgatt
     9361 gcttcactat tgaaccggat ctgccgttca aatcattcct gtaatctgtc gtttataccg
     9421 gatagcgtgg agaagttcgc gctatccggc gtagtatttc ctggctattt caaatatcat
     9481 ctaaaaaaat caaattttat cggaatagct catgactgaa gcacaaagac atcaaatcct
     9541 cctggaaatg ctcgcacaat tgggctttgt gaccgttgag aaagtcgttg agcgtctggg
     9601 aatttcgcct gccactgcgc gacgcgatat caataaactt gacgaaagcg gcaaactcaa
     9661 aaaggtgcgc aatggcgcag aagctattac ccaacagcgc ccgcgctgga cgccgatgaa
     9721 tctgcatcag gcgcagaatc acgatgaaaa agtacgtatt gctaaagcgg cctcgcagct
     9781 ggttaatccg ggcgaaagcg tagtcatcaa ctgcggctcc accgcgtttc tgcttgggcg
     9841 ggaaatgtgt ggcaagccag tgcaaatcat cactaattat ctaccgctgg caaattacct
     9901 gatcgatcaa gaacatgaca gcgtgatcat tatgggtgga cagtacaaca aaagtcagtc
     9961 catcacttta agcccgcagg gcagcgaaaa cagtctctat gccgggcact ggatgtttac
    10021 cagcggaaaa gggctgaccg cagaagggtt gtataaaacc gatatgctga cagcaatggc
    10081 agagcagaag atgctgagcg tggtagggaa actggtggta ctggttgata gcagtaagat
    10141 tggcgaacgc gcgggaatgc tttttagccg tgccgatcaa atcgatatgc ttatcaccgg
    10201 caaaaatgct aacccggaaa tcctgcaaca actggaagcg caaggggtca gcattctgcg
    10261 tgtttaaaga tgctggcgga aaaatgtcac ggcagcatct aacgcctcag gcgtaatgcg
    10321 gtgacgcacg cctggctgcc atgaacaggt tagcagttta tcccgtcccg tctcgcttaa
    10381 ggcctgctgc aaacgtagtg attcgtcggc aggcacaacg tcatcgtcga ggccatgcca
    10441 cagcagtaga ggtctgtccc caagttgttc caggtggttt gtcgcttccc actctgccag
    10501 cggcgcgata atgttattga attcattctg ctgtgctgcc gtttcaggta tcagcggtgg
    10561 aaacagtgaa cgggcgagcg atgtaaaata gcccgatccc atcatgctgg cggtacattt
    10621 caccgtgggg tggcgagcgg taattcccag tgccgtcatc gcgcccatcg acgcgccacc
    10681 gactgccaga cggtcatcaa gcagccagtt ttcttcggct attgccgcac gtaaagtagt
    10741 gaattcctgc atactttgta gcaagatttg ccagaattga tttaaccgcc gcgcttcgtc
    10801 gctactaaaa cggctaccgt gatcgggcgc atccggcatg atcacccgca aaccagcctg
    10861 cgccagcgca acggcaaaat agctatacac caggctggat gaagtaaagc cgtgataaaa
    10921 aattacgcac ggtaacgggg tatctttttg cccgacagga taagcatgaa gaacgggaat
    10981 atctgccagc tcgcgtgatt ctatttcaat catctgtctc tcctttttgt ctggtatgac
    11041 atgaagaaat ccttagcaca agtttctgct ggcggtatta gttcataatg ttgagatgtg
    11101 ggttacgctt tcgtcacgtt ttcattcgaa aatcgcgtac caggtaacaa ttcgggaaca
    11161 ttcccccaaa gttaaattaa taaggcacta cactatggtt agcaggaaac gtaatagcgt
    11221 tatttaccgg tttgccagtt tattattggt gttgatgtta agtgcctgta gcgcactgca
    11281 aggtacgcca cagccagcac cgccagtaac gaaccatccg caagagattc gccgcgacca
    11341 gacgcaaggg ttacagcgaa taggtagcgt aagcacgatg gttcggggtt ctccggatga
    11401 cgcattagca gaaatcagag caaaagctgt cgctgcaaaa gctgattatt acgttgtcgt
    11461 catggtggac gaaaccatcg tgacaggaca gtggtattca caagccattt tgtaccgtaa
    11521 ataaatcaga caacttacat agtcagcttt acattgcttt gcgtccctat gcgtttacct
    11581 gtgcacaata aattaccggc tggaagcctg ggcgtttatt accgcgatgg aatgccctgc
    11641 aacatgtggg gaaacgaaaa atggagctga cgatgaaaca attacttgct tcaccctcgc
    11701 tgcaattagt gacttatcct gcgagcgcca cggcacagtc tgcagaattc gccagtgctg
    11761 actgcgtgac gggcctgaat gaaataggtc agatctcagt tagcaatatt tcaggggatc
    11821 cgcaggacgt ggaacgtatc gtggcgttaa aggccgatga acaaggtgcg tcatggtacc
    11881 gcattattac catgtacgaa gaccagcagc ctgataactg gcgcgtacag gcgatactgt
    11941 atgcatgagt gtcatctaca gcaaatgaac aatgccggat tcggcgcgag cgccttgtcc
    12001 ggcctacaat tttcacatat ttcattgagt taatcgaaac cagcgtcgca tcagtcgtat
    12061 acgcttacac acacactccc cccgtcgccc gcagcaataa atcattctgg atctgctctg
    12121 acaaccgtac gccgccgcgc gtatctaaca tcacctgaca ccacgcctgc gccattggcg
    12181 gagaagcata tttcaacatt tgcgcaccgc agcccagcag gaatagctga tgagtaatct
    12241 ctcgccccag ttcttcagct ggcttacgca gctgctgctg taaacgacga accgcgcgat
    12301 caaaatagcg atcctgccct ttcacttcca caaatgcttc cgacaataag tcgtatacgc
    12361 ccgcttgctt attgagaacg cgcaacacat ccaggcacat aatattgccg gaaccttccc
    12421 aaatactgtt taccggcatc tcccggtaaa gccgcggcag ctcgctctcc tcgcaataac
    12481 caatgccgcc cagcacctcc atcgcttcgg ccacaaacgg catacctctt ttgcagatca
    12541 caaattttgc cgcaggcgta aataaacgcg cccacagggc ttctttggca tcggcacacc
    12601 ggtcccacgc tcgcgcaaga cgaaacagca acgccgtttg cccttcaagc tgaagtgcca
    12661 tgcgacttaa gacatgacac ataaggggct gttggatcaa tggattacca aaaacatggc
    12721 gttgatgtgc atgataaatc gccagcgaaa atgcacggcg catcatggca tggctaccca
    12781 gggcgcaatc aaaacgcgtc atcccaccca ttttcaggat cagacgaatt ccttcccctt
    12841 cctgccccaa caaccagcca atggcatcct gaaactccac ttcgcaactg gcgttagagc
    12901 gattacccag cttatctttc agccgctcga ggcgaatcgc gttgcgttgc ccatcaggca
    12961 aaaagcgcgg cacaaaaaag caggacaaac cgcccgtagt ctgcgccagc accagatgcg
    13021 catcgctttg cggcaccgag aaaaaccatt tatgccccac cagccgataa gagccatctt
    13081 ccagacgctc tgcacgggtg gtgttgctca tgacatcgga accgccctgc ttttccgtca
    13141 ttcccatgcc aatcaacaaa ccgcgttttt gcccacctgg caataagaga gaatcgtagc
    13201 gatcgctcag cagcggcgtg gtccagtcct gaaacggcgt gggtaacatc tgtaacaaca
    13261 atggcgtggc ggcaaaggtc atcgttattg gacataacga cccagcctca acctgcgcat
    13321 gtaacataaa acgcgccgcg cgcgccacaa atgcgccgga gcgagcgtct tcttcccagg
    13381 caagattgtg cacccgattg gtacataacg cctgcatcag caggtgccag gcggggtgaa
    13441 aacgcacatc gtccagacgg cgtccttgcg catcgtagcg caataattca ggcggattca
    13501 cattcgccag ccgccccagt tcaagagatt cagccgttcc taactgctga ccaatactgg
    13561 ctagaaaatc gctatcccag ccagcacctt cacgcgttac cgcttcacag agcgcgccat
    13621 cagacaggta taagttgctg ttatttaatg gtataggttg attaaaaacg gtgtgagttt
    13681 gccagtgcac tgtgtctccc tccatcaatg gcagtcaccc ttagtatggt cactgccatg
    13741 gattcctgac agaaagagat tgctaaaaca ttcttatcca gcaatgtaat gcggaacgaa
    13801 acgtgaggtg tctttggtga tcagcgtgtt atcttcacgg atccccattc cgcacgcttc
    13861 atcatcgaca atccagctac cgatgagtgt gtagctatcg ccaaatcgcg gcagaggttg
    13921 aaacgcctgg tagatcatcg gttcatcggc gtaatcacca tcagcgtggt caacgacgtt
    13981 attctgaccg tcgaaaatgg tgacgtttcc gccttcgcgt gagtagatcg gtttacgcac
    14041 atagctttcg ccagcggcaa tctgcggttt ttcgccctca aaccaggatg caagaaggtt
    14101 aggatgacca ggaaagaagc gccaaagcag cggcattagc cctttattac tcaagatact
    14161 tttccataac ggctccaccc attgctcgcg acgcttgcgc agcagcggac cgttatcgtc
    14221 acgcatcatc cactccagcg gatacagctt aaatgcacgc tggatgacat tatcatcaag
    14281 atcggtcagt acgccgccga cgcccaaacc gagatcttca atgtagataa accgcgactc
    14341 ctgccctgcc tgctgggcgc agtcctgcaa gtacagcacg gtactcctgt cttcatcggt
    14401 gtcctgacag cagcaaaaat aaaacggttc ccgactgtaa agctcactaa agcgcgaaat
    14461 caggcgttcc tgaatggcgt tgtactccag cagcttcacc ggcgcattgc cacaccaggc
    14521 aaaatccatc cggccataca gcgaaggatc gcgtgcgcgc cagctttcgg cgatcacatc
    14581 ccagtacaac ggcgggatcg ccagttgcgt caggatctct tcgtctttaa ccgcgcgatc
    14641 caccacctca aggcacatct gatgcagttc cgcagtcggt ttttcgatct gctcttcaat
    14701 ctggcgcaga gtaaaacggt aagcccgact ctcgtcccaa tagatttcat tgtcgatgat
    14761 atgaaagtcg aaaccgttgt tagcggcgat ctggtccaga tcccgtcgca caggaacgtt
    14821 gtgtctcagc atgattagcc tccccaatgc ccacgggcgc tggaagaacg accatagccg
    14881 ccgcgcgaaa cggttgacgc ttttttggtg gtaaagccgc ctgaagagta agactctttt
    14941 ttgccagaac cggagcgcca ggagtaatca ccggaagtgt tgagccagac ggggcgggaa
    15001 gcaaatgagg atccgccgct gttatagacg aacggctcat cgcgatcttt acgaataacc
    15061 cggcttaaca aaaatccaga aacgaccggg atccaactct gttcaacatt gtcgtagtag
    15121 caattttcgt acttagactg acaattctgc tgagtcatat tcttcggaac atcggcataa
    15181 aatgccgctt tggcgttgtt ccagccacgc gcgcaaatgt cggcattatt accgtcatca
    15241 atacaatcct gcactgtcga ataaaacgtt ccgtcgccgt cgttatcgac atcgccacta
    15301 tcgccacaac ctttcaatac aaaaaaagcg gcaccgccca ttagcgccaa cgttaagtat
    15361 ttttgtgcgt agcgattgcg acgcggttca aaaggattat tcggtctacc aatgcgactg
    15421 atcgctccgt gaccaatttt actgttattt cttgatttgc gttttctggc catgatcgtc
    15481 cattaccatg tcatacaagc tgcgttaaaa ataccgccag ccagcgcggc ggttcccata
    15541 aacattcctg ctgcggtatt gtgattaata attttttcgc ttaatgcggg catataaagt
    15601 ctgacaccag caaaaacgag taactgaatc accagtgcaa tcccgcccca ggcgaaatag
    15661 tctggaatac tcaccgcatt gattgccgca ctggataagg ggatcacgta acccaacaat
    15721 gtaccgctga atgccagtga caccgcggta ttattgtttt tgattaattg ccattcgttg
    15781 tgcggtgtaa ttttggagta gataaacaga aaaataatca ccatagccac gccaataaaa
    15841 aaataggcac taaaggcaag aagtgaatcc agtatatgca ttgtaaacgt ccttgtaatt
    15901 aaccaataat atgaagtgat gtcagcggga tatcgacacc caacgcgcgc gaaaataacc
    15961 actctggctc gccgagatcg ttaaaagatt cctcaccatt taacagtaaa tattcgtaag
    16021 tatcttcggt tacttgccgc tggtagccca tggtaaaatt atggacctcc catttggcat
    16081 ggttttgatt ttctactttt tccagcatgt agacgggttc gatatttccc ggttcttcgc
    16141 tgttaaaaaa tcgctgccag cgtttttcct gccagtttaa ggtcattgcc cccatcgttt
    16201 tggcgttgat cgcctcgcgc cagtgactct ctttactgat accgtagctc tcttcataga
    16261 caaagagctt gatatcatca atgtcatcaa tatcttcgcc gccggtggta ttgatttgca
    16321 gaaattcatc gcctgaagtg tagtaacgga aaatctggct accgcctccc agatcgatgc
    16381 ggctgacagc ggctaccgta aattcctcac ccggtagcgc aatcagcaat tcatcttcca
    16441 gcaaacgaaa cgctaacgtg tcgagcgtaa agccactatt gagatgaagt cccagcggac
    16501 cgcgagcgat agcaggctta ttatccttgc caaacagacg ctggaaaaaa ccagacatat
    16561 tttatcctta aaatagttgc cagcctttta gcgaggctgg caaaaaaatt actcgccctg
    16621 ttggcgttgc agtcttgcta atacatcctg ggctctactt ttattgctac cgccaattcc
    16681 ggcttccgcc agcttttcgt caaggtcgcg accgtctgcg actttctcca actgtgcggc
    16741 agcatccagg cgagcctgac gttcggcctg acgcgtttgc aggcgtttta aggattctgc
    16801 cgctgtcgaa acgctggaag atgcgccaac ggtagaggtt gttacagcct gttgtgcacg
    16861 ctgcatggct tcagtggctt taacgacttc catttgctgc tcaaactgag cgatacgctg
    16921 cgctgtcgct gtaactgctt tttccacgcc atcacgggag acttccagat tcgacaacac
    16981 ttgctcttca gcggtaatga gattctcaag gcgcgcgatt tcttcagcaa cttcgttaat
    17041 caacgacgga ttaacgttct tgcttaacgc ttccagcgca cgggcttcca gactggcttt
    17101 gcgctcacgt aaatctttca gcttatcgtg acttaatttc acccgcgcca gcagatcaac
    17161 gcgagatttt ccggctttat cgagttcagc tttagcgtca cgaatatgct gttccagcat
    17221 gcgcacgccc tgggtttctt caatggattc ttccgcctgg gagataaacg atttccccag
    17281 cgtaaataaa ctttttaaaa ttcccataaa tacccgtcct tgtaattagt gtgaatattc
    17341 ttctgtaatt tcagccagat ccaatgcgtt atctaccagc taggttatct caagcaggat
    17401 atcttcaaga gaagatttaa gcgataacgc cccgaaaaca atgtaatact cttcctgttg
    17461 cacgctggag attcctaccg atgacaacgg catcatcttc tga
//
LOCUS       DADLFA010000078        17502 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000078 DADLFA010000000
VERSION     DADLFA010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17502
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.078"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..321)
                     /locus_tag="JQH65_002217"
     CDS             complement(<1..321)
                     /locus_tag="JQH65_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BAX inhibitor (BI)-1/YccA family protein"
                     /protein_id="HAY6139615.1"
                     /translation="MESYSQNSNKLDFQHETRILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPTMYVAIGLLLVCMYGLSKDINKINAVIAGVIYLFLLSLVAIVVASLVP
                     VYAII"
     gene            complement(527..1231)
                     /locus_tag="JQH65_002218"
     CDS             complement(527..1231)
                     /locus_tag="JQH65_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAY6139616.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(1368..1820)
                     /gene="moaE"
                     /locus_tag="JQH65_002219"
     CDS             complement(1368..1820)
                     /gene="moaE"
                     /locus_tag="JQH65_002219"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HAY6139617.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFAGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(1822..2067)
                     /gene="moaD"
                     /locus_tag="JQH65_002220"
     CDS             complement(1822..2067)
                     /gene="moaD"
                     /locus_tag="JQH65_002220"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HAY6139618.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHMAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(2060..2545)
                     /gene="moaC"
                     /locus_tag="JQH65_002221"
     CDS             complement(2060..2545)
                     /gene="moaC"
                     /locus_tag="JQH65_002221"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HAY6139619.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARITGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2548..3060)
                     /gene="moaB"
                     /locus_tag="JQH65_002222"
     CDS             complement(2548..3060)
                     /gene="moaB"
                     /locus_tag="JQH65_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HAY6139620.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGEQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(3082..4071)
                     /gene="moaA"
                     /locus_tag="JQH65_002223"
     CDS             complement(3082..4071)
                     /gene="moaA"
                     /locus_tag="JQH65_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HAY6139621.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(4059..4203)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4468..5376
                     /gene="yvcK"
                     /locus_tag="JQH65_002224"
     CDS             4468..5376
                     /gene="yvcK"
                     /locus_tag="JQH65_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HAY6139622.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(5568..7589)
                     /gene="uvrB"
                     /locus_tag="JQH65_002225"
     CDS             complement(5568..7589)
                     /gene="uvrB"
                     /locus_tag="JQH65_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HAY6139623.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(8168..8845)
                     /gene="bioD"
                     /locus_tag="JQH65_002226"
     CDS             complement(8168..8845)
                     /gene="bioD"
                     /locus_tag="JQH65_002226"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HAY6139624.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(8838..9593)
                     /gene="bioC"
                     /locus_tag="JQH65_002227"
     CDS             complement(8838..9593)
                     /gene="bioC"
                     /locus_tag="JQH65_002227"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HAY6139625.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRRSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(9580..10734)
                     /gene="bioF"
                     /locus_tag="JQH65_002228"
     CDS             complement(9580..10734)
                     /gene="bioF"
                     /locus_tag="JQH65_002228"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HAY6139626.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFVHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYNTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(10731..11771)
                     /gene="bioB"
                     /locus_tag="JQH65_002229"
     CDS             complement(10731..11771)
                     /gene="bioB"
                     /locus_tag="JQH65_002229"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HAY6139627.1"
                     /translation="MAHRPRWTLSQVAELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPNERDMPYLEQMVQGVKDLGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            11858..13147
                     /gene="bioA"
                     /locus_tag="JQH65_002230"
     CDS             11858..13147
                     /gene="bioA"
                     /locus_tag="JQH65_002230"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HAY6139628.1"
                     /translation="MTKDDLAFDQRHIWHPYTSMTSPLPVYPVVSAEGCELILSDGRR
                     LIDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEVRQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHNLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            13206..13682
                     /locus_tag="JQH65_002231"
     CDS             13206..13682
                     /locus_tag="JQH65_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HAY6139629.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVEHIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            13834..15117
                     /locus_tag="JQH65_002232"
     CDS             13834..15117
                     /locus_tag="JQH65_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HAY6139630.1"
                     /translation="MNTFSVSRLALALAFSVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPNFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(15258..17495)
                     /locus_tag="JQH65_002233"
     CDS             complement(15258..17495)
                     /locus_tag="JQH65_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HAY6139631.1"
                     /translation="MFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSSHNTSGNMD
                     KLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGTINGDDHVF
                     GLSAAQRYGGIFVPPHIAIIHQYMREMMAGGGKMILGSDSHTRYGALGTMAVGEGGGE
                     LVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVKNKVMEFVG
                     PGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQLNPQPMAYY
                     DGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAHGKAKLSLL
                     DKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPSSQPVFMDL
                     AKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREGSKPANGQM
                     SAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQGFVKGATQQ
                     PLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRSNPIGLAEF
                     TLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMV
                     YAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEV
                     GDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFN
                     KNRQM"
BASE COUNT         4093 a         4734 c         4440 g         4235 t
ORIGIN      
        1 gataatggca tatacaggaa ctaaacttgc aacgacaatc gccaccaaag agagtaaaaa
       61 cagatatatt acgcctgcga tgacggcatt tatcttgttg atatccttac ttaagccata
      121 catacaaact aataataacc ctatggcgac atacattgtt ggtgaatcat atttggttgc
      181 cgtaatttcg agatacttag ctccccaagc cagtcctgcg gttgccacca aacctaaagc
      241 ggtaatgagc catataccgt ttaatatcct ggtctcgtgc tgaaaatcta atttattgct
      301 gttttgtgag tatgattcca ttcttttagt tgtccttaga aaaaatatta atattcacca
      361 tcaataaatt taaacccacg catacaaaat acttttgtat ttatggatac ggaaaacgct
      421 tccgctcggc cataaggcgt attttatacc tggtacttgt taagtgtcgt ttttagtaag
      481 aaaagaaatc ccgacacatg gccgggatat tgatggtgaa aaagaattaa cggcggttgc
      541 caaaaattcg cagcaacatc aggaacaggt tgatgaagtc cagatacaag gttaacgcgc
      601 caagaatgga atatttgcgc aggttcgacg tgtcgcgggt atcaatctgc tcacccatat
      661 ttttcaattt ctgagtgtca tacgccgtca atccgacaaa gacaatcacg ccgatgtagg
      721 taactgccca catcaatgct tcgcttttca gccagaagtt gaccagcgat gccagcacaa
      781 tgccgattaa cgccataaac agcatattgc caaagccact taaatcgcgc ttcgtggtgt
      841 aaccgtacag gctcattgcg ccgaacatcc cggcagtaac gacgaaagta ctggcgatag
      901 aagcagcggt atagacaatg aatatactgg aaagcgtaag acccgtcagc gccgaataaa
      961 gcataaagag catcgtcgtt acacctgcgc tcagcttttg aatcatcgct gataacacaa
     1021 taaccaatgc taattgcgcg atgatcagac cgattaaaaa gacacggtta gtgaacaaca
     1081 gctccatcac ggccgcggaa ttagccgcat accaggcaac aaatgcggtc agcaacaagc
     1141 caacggtcat ccagccatag acttgcgcca tataagtttg caagccagcc cggggttgta
     1201 cgattgaatc agaacgtggg aatctgtcca tgacgatctc ctgaagatat aaggaatatc
     1261 ttaaggatac tgcaaaatga tgaggctgtg catcgacgca gcgtaaacgc atgtactgag
     1321 cggtgaaatt gccggacgca gcggtgcctt atccggctaa caaaaaacta ccagcgtttt
     1381 gccgcctgct gatcgctctc ccgagcttca acccagcggt cgccttccgg cgtggcttcg
     1441 cgcttccaga acggtgcgcg ggttttgaga taatccataa taaactgccc ggcttcaaac
     1501 gcactgctgc gatgcgcact ggtgacaccg gcaaaaacga tttcatcgcc cggccataat
     1561 tccccgatgc ggtgaatcac agtgacgcgc cccagcggcc agcggttacg cgcttcatca
     1621 acaatttctg ccagtgcttt ttcagtcatt cccggatagt gttcgagggt taatgctttg
     1681 acgctgtcgc ccaggttatg gttgcgcacc ttaccagtaa aggtgactac cgcaccgtct
     1741 tcgtcacgct ccgccagcca cgggtactct tctcctacgc tgaacggctg cggaccaaca
     1801 acaattttgg tttctgccat cttaacctcc ggttaccggc gggaagaaag ctacttcgtc
     1861 gccgtcagtc agcggatggt caaaactcac cagcgtctgg ttgacggcag ccagtaattt
     1921 gccatcttcc agcgccagcg cccagcgatc gctctgcgca gccatgtgct ggcgtaacgc
     1981 ttcaacagtt gggaaatccg cagccacttc ggttgcatct gttcccacca actcgcgcac
     2041 ctgcgcgaaa aaaagaactt taatcatccg cttccacctt aaagtcaccc gacttgccgc
     2101 cgctcttcgc cagcaaacgt accggaccaa tcaccatatc tttttgcacc gctttgcaca
     2161 tgtcataaat ggtcagcgcc gccacggagg ccgcggttaa tgcttccatt tcgacaccgg
     2221 ttttcccggt caggcggcat aaggtttcta tacgcacccg attgtgctcc ggctcggcct
     2281 gtaaattgac ttcaactttg ctgagcatca gcggatgaca gagcgggatc agatcccagg
     2341 tgcgttttgc cgcctgaata ccggtaatac gcgcagtggc aaatacgtcg cctttgtggt
     2401 ggcgaccatc aataatcatc gccagcgtct cgctgcgcat ggtgacaaag gcttccgccc
     2461 gcgcttcacg cacggtttcc gctttggcgg agacatccac catgtgcgct tcgccagcgg
     2521 cgttgatatg ggtcagttgc gacatactta tttcttcaaa tgtggatgga aattacacgg
     2581 acgcgtacgg gcatccagct gcggcgcgat gatattttcc catgcggtac ggcacgcttt
     2641 ggtcgaaccc ggcatggcga aaatcagcgt tttgttggcg acgcccgcaa ccgcacgaga
     2701 ttgcaacgtg gaagtgccaa tctcttcaaa cgacaacatc cggaacactt caccaaaacc
     2761 ttcaacttca cggtcgaaca acggcagcaa tgcttcggga gcctgctcac cttccgtcag
     2821 gccagtacca cccgtaatca ataccacttg tacatcgtcg ctggcgatcc acgcagatac
     2881 ctgagcgcga atagcgtagc ggttttcttt cacaatggct ttatcgacaa cgtgatggcc
     2941 cgcttcttgc gccgaatcgc gcagatagtg accggaggta tcgtcttctt caccgcgacg
     3001 attagaaacc gtaagaatag caatacgggt cgggataaat tcagtgctta cctgactcat
     3061 ctgatctctc cttttgacgt tttagccgcc aatgtacgat aagttttgcg taataccggt
     3121 gttgttttga tgcaggaaat gggtctgttt cttctcccgc agcgccgctg aaatacgcgc
     3181 ttccagcgcc tgttgctggg tatcgtcttc cagcagatcg cgcaggttaa cgccgccttc
     3241 accaaacagg cagagatgga gtttaccaat ggaggaaacg cgcaggcggt tgcaagtggc
     3301 gcagaagtct ttttcatacg gcatgataag gccaatctct ccggcgtaat ctggatggca
     3361 aaagacttgc gcgggaccgt cgctgcgttg acgtaattgg tggatccagc cgcgacgcag
     3421 tagctcgtca cgcagaacct gaccagagat gtgatgctta cggaagagct cgctgccctc
     3481 gcccgtttcc atcagttcga tgaaacgcag ctggataggg cgatgctgga tccagttcag
     3541 aaaggtgtcg agctggtgat gattaacatc acgcatcagc acggtattga ctttgacctt
     3601 ctcaaaaccg gcctcaaatg cagcatcaat ccctgccatg acctggttga atttatcctg
     3661 cccggtaata gcgtgaaact ggcgggcgtc cagactgtcg acactgacgt taatgccagt
     3721 aagtcccgca tcgcgccagt tcgccacatc gcgttccaga cggtaaccat tggtggtgac
     3781 cgcaatctgg cggatagcgt cgttttcccg cacagcggcg atgatatcgg taaagtcgcg
     3841 gcgtaaagac ggctcacctc ctgtcaggcg cactttttcg gtgcccagac tggcgaaggc
     3901 gcgcgtaacc cggcgaattt catcgacggt aagaaagcct ttattggtga cgccgctcgg
     3961 tttgtagcca tccggcaggc agtaggtgca acgaaagtta cacacatcgg taatcgacag
     4021 gcgcaagtag taaaacttac gcgcaaatgc atcagtcagt tgtgaagcca tgtacacctt
     4081 tccagatacg ggaggcgaag tcatttcttc cttcgccctg gtggcaatct ttacgagcat
     4141 tgtcacggcc aaagcaccgt atcagttgac ccaggtgcag aggctagagt gtttagtggt
     4201 taagccgata ctagcgtgta aatgagattt ttaccatcca cactttcgct atgcaatcat
     4261 gtatatagcg tcatgatcgt gctatttcgc tacagaataa gggaaaatgg cagttttact
     4321 ttgatataca tcatcaggga ggcatagcgt gcatcgtcct ttagaggtag aagaaaatag
     4381 tttgtatcct caatattggc aggttaattg ctgtttcccc gcaatttgcg ctactgtagc
     4441 gcggataatc tgactccagg aaactatatg cgcaatcgta cgctggctga tcttgatcgt
     4501 gtcgttgctc tcggcggagg gcatggactg ggacgcgttc tctcatcact ttcgtctttg
     4561 ggttctcgtt taacgggtat cgtcaccacc acggataatg gtggctcgac ggggcgtatt
     4621 cgccgttcag aaggcggcat tgcctggggc gatatgcgca actgcctcaa ccaactgata
     4681 acggaaccga gcgtcgcctc cgcgatgttt gaataccgtt ttggcggcaa tggcgaactt
     4741 tccgggcaca accttggaaa cttgatgtta aaggcgctgg atcaccttag cgtgcggcct
     4801 ctggaagcca tcaatttaat tcgtaatctg ctgaaagtgg atacgcattt gattccaatg
     4861 tcagagcatc ccgtcgatct gatggcgatt gacgatcagg ggcatgaagt ttacggcgag
     4921 gtcaatatcg accagttaac tacgccgatt caagagttat tattaacgcc gaatgtaccc
     4981 gcaacgcgtg aggcagttca cgctatcaat gaagcggatc tcatcattat tgggcctggc
     5041 agtttttata ccagcctgat gccaattctg ctgctgaagg aaatcgccca ggcattacgc
     5101 cgcacgccag cgccgatggt ttatatcggc aatctggggc gtgagttgag tttacctgcg
     5161 gctaatttga agctggaaag caagctggca attatggagc agtatgttgg taaaaaagtc
     5221 attgatgcgg tcatcgtcgg gccaaaagtg gatgtctcgg cggtgaaaga gcggattgtg
     5281 atccaggagg tactggaggc cagcgatatt ccgtatcgtc atgaccgcca gttgttacat
     5341 aacgcgctgg aaaaggcgtt acaggcttta ggttaacgga aatccgaagg aaaattccgg
     5401 cttcctattg aagacaaagt gcgcgttgtt tatgccggat gcggcgtgaa cgccttatcc
     5461 ggcctacaaa ccgcgcaaat tcaatatatt gcggagaaaa tgtaggcctg ataagcgtag
     5521 cgcatcaggc tgttttccgt ttgtcatcag tcttcttcgc tatcctgtta cgatgcggca
     5581 ataaacaact cacgcaactg atgcaactgg tcacgaattt gtgccgcttc ttcgaactcc
     5641 agattctgcg cgtgttgcat catcagccct tccagttcat ggattttctg ctgcaacgct
     5701 ttcggcgaca tatccatcgg cacattatcc ggctcaacaa tcgggcgcga ttttcctctg
     5761 cccttcgctt tggttttggc aatgttctgc cccagcgcca ggatatcgac cactttcttg
     5821 ttcaagcctt gcggcgtaat gccgtgttcc tcgttgtact tctgctgttt ctcacggcga
     5881 cgttcggttt cgccaatcgc tttcgccatt gatggggtga tcttatcgcc gtagagaatc
     5941 gctttaccgt taacgttacg tgccgcacga ccaatggtct ggatcaacga acgttcggaa
     6001 cgcaggaagc cttctttgtc agcgtcgagg atcgccacca gcgacacttc cggcatatcc
     6061 agaccttcgc gcagtaagtt gatccctacc agcacgtcga actcacccag acgcaagtcg
     6121 cggataatct ccatacgttc gacggtgtcg atatctgagt gaagataacg cacgcgctcg
     6181 ccatgttctt cgagatattc ggtaagatct tccgccatcc gcttggtcag tgtggtgacc
     6241 agtacgcgtt cgttaattgc cgctcgctga cgaatctccg aaagaagatc atcaacctgt
     6301 gtcgccaccg gccgcacttc gataatcggg tcaagcaatc cggttggacg caccacctga
     6361 tccaccacat cgccgccgga tttttccagc tcgtaattac ccggcgtcgc cgaaacatag
     6421 atggtttgcg gcgctaatgc ttcgaactct tcaaacttaa gcggacggtt atccagcgct
     6481 gatggcaggc ggaagccgta ctccaccagt gtctctttac gcgcccggtc accgcgatac
     6541 atgccgccaa tttgtggaat ggtgacgtga gattcatcga cgaccagcag cccatcggca
     6601 ggcaggtaat caaacagcgt cggcggtggc tcacccggtc cacgaccgga gaggaagcgc
     6661 gagtagtttt caatccccga acagtagccc agctcgttca tcatctccag atcaaactgg
     6721 gtacgctggg tcagccgctg ctcttccagc agtttgttgt tttccaacag cactttgcgt
     6781 ctggcggcca gttcttcttt gatctcctcc atcgcctgta cgatgcgctc gcgcggtgtg
     6841 acgtagtgcg ttttcgggta gatggtaaaa cgtggaatag tggaaacaat ctgcccggtc
     6901 agcgggtcaa ataacgacaa tcgttccact tcctcgtcaa acagttccac gcgaagtgca
     6961 atgtcatccg attctgccgg gaagatatca atcacctcgc cacgaacgcg gaaagtacca
     7021 cgctggaatg cttgatcatt acgagcgtat tgcagctccg ccagtcggcg cagaatcgcg
     7081 cgctgatcga taatcatacc gaccgtgaga tggagcatca tcttgagata taaatcagga
     7141 tcgcccagac cataaatcgc ggaaacagag gccactacaa ccacatcacg ccgctccagc
     7201 atcgctttgg tggcggataa acgcatctgc tcaatatgtt cgttaaccga ggcatctttc
     7261 tcaatgaaag tgtcggaact cggtacatag gcttccggct gatagtagtc gtagtaggag
     7321 acgaaatact ccaccgcgtt ttccgggaag aactctttca tttcgccata cagctgggcc
     7381 gccagcgttt tgttgggcgc aagtaccatg gttgggcgct gaaggtcagc aatgacattg
     7441 gcaatggtga aggttttccc tgagccagtc acgccaagta acgtctggtg cgccaggcca
     7501 tcttccagcc cctcttcgag acgtcgaatc gcctctggct gatcgccaga aggtttaaaa
     7561 gcggaattca gtttgaacgg tttactcatg agtcgctacc tgaaggagtt gggcgggcag
     7621 gtatgtaatt ttactcgtcg tacttaatta tgccaacaaa ttatactgga taaaaaaaca
     7681 gttcatcacc ataatatttc tgatacagcg taaactccgc tgtcaatcat gagcaaaatt
     7741 tactctgtgg cgagataaaa ctccggcctt accgggttat ccccaaagca acggcttttt
     7801 taacatttgt caagatgagt gatgacagtt ttatggcaag agatgcctgt tcagtgactg
     7861 ccattgcatt tatctaacca gttaaaaaat aaaagatatt tctttgagcc gtctttaacg
     7921 ccaattcgcg tgcaagccgc gtattctctc gcttgcctcg tgttttctaa ctctaataca
     7981 catggttatc cacaggaata gtggataact gtctccagcc cctatccccc gccgcctggg
     8041 caattcccac attcgccgca tgaggcggaa gtaaaatttt tttcgtgatt attttagaat
     8101 ttaattggtt aaattgcagt caatcgaaga cgcgatctcg ctcgcaattt aaccaaatac
     8161 agaatggcta caacaaggca aggtttatgt actttccggt tgccgcattt tctggatttt
     8221 ctgcaagcca ggggatctct cccagcagcg gcgcgggaat catgcgggtg agcgtggtca
     8281 tatattcagc gtgacgtttt cccggaggcg taacatcgtt cgccacccaa cccgccagag
     8341 tcagtccggc gtgttgtatt gcctgcgcag tcaacatcgc gtgattaata cagccgagtt
     8401 tcacaccaac taccagtatc accggcagtt gttcctgtgt tacccaatct gcaaaagtga
     8461 aagtgtcaga aagcggcgta aaccagccgc cagcaccttc cactaacacc cagtcagcct
     8521 gttgttcaag cgcgcgtaat ccggcgctca ttaccaatga ttctatcggc ctgccctctt
     8581 gcgcgctgat gatgtgcggc gaagtgggtt ctgcgaaggt gtaaggattt actgttgcgt
     8641 aatccagctg caggctgctg ttgtgctgta acgccagcgc gtcactatta cgtaagcctt
     8701 ccggggtctt ttcgctgcca gaggcgaccg gtttataacc tgccgtccga taacctactg
     8761 ccttcgcggc ttgtaaaagt gcacaactgg cgacggtttt ccccacttcg gtatccgttc
     8821 cggtgacaaa ataacgttta ctcacgagca atcactccca aaaaaagatg atacgtcaga
     8881 ggatatcgcc cctgttgttg cggccaggcc agttgcaatc gctgcaattg cgaacgcgtt
     8941 aatattcgcg ggtcgcgccc ttcatgaaga tgcgtagcac cgatgccttt cagcgaacgc
     9001 atggcactga gcgcatcatc aaaccatagt gtgatgggct gaatatgatg ttgataatgc
     9061 acgccgttca gcgactgttc gatttcatct ggcggtaaaa agcgattagc atgcggacgc
     9121 tcgtccaccg cctgccacgc ctgatgcagt tcgggtaacg atccctgcac cagcgtggta
     9181 aacgccacca cgcctccggg gcgcaccacc cgatacagct cgcggagtgc cgtggataaa
     9241 ttaccgcacc actgcaccgc gagattgctc catgcaagat cgaacgtcgc agtcgctaac
     9301 ggcagggatt cgatatctcc cgccagataa tggtctgcag catctttctg gcgggcttgc
     9361 acaagcattg gcggtgagag atctaacgcc gtcacctgtg cgtgacgctc tcgccagtgg
     9421 cggctcatcc agcctggccc acaccccgcg tccagtacgt gggtgtattt acgctgtggg
     9481 agcattgcca gtaagacgtc agcactccgg cgctgtaact ctgcgtgttg ctcatagtgc
     9541 gcggctgccc gaccaaatgc cgctgcaatg gcttgtttat taaccgttgc catgcagcac
     9601 ctccagcaga cggtcgatat cctgcatttc atgcgctgcg gttagcgtta agcgcagtcg
     9661 cgcagtacca gcgggtacgg ttggcgggcg aatcgccgtg acccagcagc cttgctggcg
     9721 cagtttttct gccagttgta acgcacggct gttatcaccg acgatcaatg gctggatggc
     9781 gctgcatgaa tcagcaagcg taaacggcaa atcctgtact ccggcacgaa aacgcgcaat
     9841 gagtgacacc agtttttcgc gccgtgcatc cccctcatca cggcgaatga ccgccagcga
     9901 cgcacgtaat gcctgcgcct gagcgggcgg catactggtg ttgtagataa ggtggcgggc
     9961 gaattgcagc agataatccg ccaccgtact ggagcaaagc accgctgccc cgctgacgcc
    10021 aaatcctttg ccaaaagtca ccaccagcaa ttctggtttt accttttgca gccagcagct
    10081 gccgcgcccc tgctccccga taacgcccgt gccgtgggca tcatcgacca tcaaccagcc
    10141 attgtgctgt tgcgttacct gctggatttc cgccagtggc gcactatcgc cgtccatgct
    10201 gaacacgcct tctgtcacca ccagttgctg ccccggacag ggggaagcaa gcagtcgcgc
    10261 cagatgagtg acatcgttat gaacaaaacg gcgaagctgc gacgggctta aactggcagc
    10321 ctccagcaat gaggcatggc taagccggtc agcgacaatg cggtcctctt ttgccatcat
    10381 cgcggcaatg accgcctgat tagcggcaaa accagagata aatagcagtg cccgcgaata
    10441 gcccagccac tcggccagtt cttcttccag cgcctgatgc gcaacgctat aaccgctgac
    10501 gtgaccggaa ccgccgctac cgacgccaaa ttgctccgca ccctgctgcc aggcacggat
    10561 aatttgcgga tgatggctta aacctaaata atcgttactg gaaaagttca gatactggcg
    10621 atcatccgcc accagccagc gtccggctcc ttgcgccact ggataacggc gacgcagggc
    10681 atcggcagca cgccgcgcat cgagcgccgc gttgattttc tcctgccagc tcatagtgct
    10741 gccgcgttgt aatattcgtc agtatccggg gtcatcagcg cctgttcaag acgttgctgt
    10801 tgttcgttat cccctgccag cacggcagtt tgctgcggat ttagccccag tttgcggaac
    10861 agttgcaggt ctttatcttc ttccggattc ggcgtggtca gcagtttgca accgtagaaa
    10921 atcgagtttg cgcctgccat aaagcacatc gcctgagtct gttcgttcat ctgctcgcgt
    10981 ccggcagaaa ggcgcacgta agaggtcggc atcatgatcc gcgcgaccgc aatggtgcga
    11041 ataaaatcaa aggcatcaac gtcatcgtta tcggcaagcg gcgtgccttt caccttcacc
    11101 agcatgttga ttggcacgct ttccggcggc gtcggcaggt ttgccagttg cagcaataat
    11161 ccggcgcgat cttttaccgt ttcgcctaag cccacaatgc cgccagaaca gactttgatc
    11221 ccggcatcgc gcactttttc cagcgtatcg aggcgttcct gataagtgcg ggtggtgatg
    11281 atattgccgt aaaactccgg cgaggtgtcg aggttgtggt tgtagtaatc cagcccggcg
    11341 ttcgcgaggc gctgcgcctg agattcactc aacgtgccca gcgtcataca cgcctccagc
    11401 cctaaatctt ttaccccctg caccatctgc tccaggtacg gcatatcgcg ttcgttggga
    11461 ttcttccacg ccgcgcccat gcaaaaacgc gtcgatcccg ccgctttcgc tttgcgcgcc
    11521 gactccagca cctgttcaac ttccatcaac cgctcggctt ccagaccggt tttatagcgc
    11581 gagctttgcg ggcaatattt gcaatcttcc gggcaagcac cggttttaat cgacagcaac
    11641 gtgctgacct gcacctgacg aggatcgaaa tgctggcgat gcacctgctg cgcttcaaac
    11701 agcagatcca gcaacggttt ttcaaataat tctgcgactt gcgacaatgt ccagcgtggg
    11761 cggtgagcca tggggcttct ccaaaacgtg ttttttgttg ttaattcggt gtagacttgt
    11821 aaacctaaat cttttcaatt tggtttacaa gtcgattatg acaaaggacg atcttgcctt
    11881 tgaccaacgc catatctggc acccatacac atccatgacc tccccgctgc cggtttatcc
    11941 ggtggtgagc gccgaaggtt gcgagctgat tttgtctgac ggcagacgcc tgattgacgg
    12001 tatgtcgtcc tggtgggcgg cgatccacgg ctacaatcac ccgcagctta atgcggcgat
    12061 gaagtcgcaa attgatgcca tgtcgcatgt gatgtttggc ggtatcaccc atgcgccagc
    12121 cattgagctg tgccgcaaac tggtggcgat gacgccgcaa ccactggaat gcgtttttct
    12181 cgcggactcc ggttccgtag cggtggaagt ggcgatgaaa atggcgttgc agtactggca
    12241 agccaaaggc gaagtgcgcc agcgttttct gaccttccgc aatggttatc atggcgatac
    12301 ctttggcgcg atgtcggtgt gcgatccgga caactcaatg cacaacctgt ggaaaggcta
    12361 tctgccagaa aacctgtttg ctcccgcccc gcaaagccgc atggatggtg aatgggatga
    12421 acgcgatatg gttggctttg cccgcctgat ggcggcgcat cgtcatgaaa tcgcggcggt
    12481 gatcattgaa ccgattgtcc agggcgcagg cgggatgcgc atgtaccatc cggaatggtt
    12541 aaaacgaatc cgcaaaatgt gcgatcgcga aggtatcttg ctgattgccg acgagatcgc
    12601 caccggattt ggtcgtaccg gcaaactgtt tgcctgtgaa tatgcagaaa tcgcgccgga
    12661 cattttgtgc ctcggtaaag ccttaaccgg cggcacaatg accctttccg ccacactcac
    12721 cacgcgcgag gttgcagaaa ccatcagtaa cggcgaagcc ggctgcttta tgcacgggcc
    12781 aacttttatg ggcaatccgc tggcctgcgc ggctgcaaac gccagcctgg cgattctcga
    12841 atctggcgac tggcagcaac aggtggcggc tattgaagta cagctgcgcg agcaacttgc
    12901 ccccgcccgt gatgccgaaa tggttgccga tgtgcgcgtg ctgggggcaa tcggcgtagt
    12961 cgaaacaact cgtccggtga atatggcggc gctgcaaaaa ttctttgtcg aacagggggt
    13021 atggatccgg ccttttggca aactgattta cctgatgccg ccctatatta ttctcccgca
    13081 acagttgcag cgtctgaccg cagcggttaa ccgtgcggtg caggatgaaa catttttttg
    13141 ccaataacgg gaagtcagcg tgtgggtttc tggctacact ttctgcaaac tagaaaggag
    13201 ggttcatgaa actcatcagt aatgatctgc gcgatggcga taagctgccg catcgtcatg
    13261 tctttaacgg catgggttac gatggcgata atatttcacc gcatctggcg tgggatgatg
    13321 ttcctgcggg aacgaaaagt tttgttgtca cctgctacga cccggatgcg ccaaccggct
    13381 ccggctggtg gcactgggta gttgttaatt tacccgctga tacccgcgta ttaccgcaag
    13441 ggtttggctc tggtctggta gcaatgccag acggcgtttt gcagacgcgt accgattttg
    13501 gtaaaaccgg atacgatggc gcagcgccgc cgaaaggcga aacccatcgc tacattttta
    13561 ccgttcacgc gctggatgta gagcatattg atgtcgatga aggagccagc ggcgcgatgg
    13621 tcgggtttaa cgttcatttc cactctctgg caagcgcatc gattaccgcg atgtttagtt
    13681 aatcactctg ccagatggcg caatgctatc tggtatcact taaaggtatt aaaaacaact
    13741 ttttgtcttt ttaccttccc gtttcgctca agttagtata aaaaagcagg cttcaacgga
    13801 ttcatttttc tatttcatag cccggagcaa cctgtgaaca cattttcagt ttcccgtctg
    13861 gcgctggcat tggcttttag cgtgacgctg accgcctgta gctcaacacc acccgatcaa
    13921 cgtccttctg atcaaactgc gcctggtacc tcttctcgcc cgattctgtc ggcaaaagaa
    13981 gcgcagaatt tcgatgctca acactatttt gcatccctga caccaggtgc tgcagcgtgg
    14041 aatccttccc cgattaccct gcctgcgcaa cctaactttg ttgtcggccc ggcgggcact
    14101 caaggtgtaa cgcataccac gattcaggcg gcggtagatg cggcaattat caagcgtacc
    14161 aacaagcgcc agtatattgc cgtgatgcct ggtgagtatc agggaacggt gtatgtccct
    14221 gctgctccgg gtggaattac tctgtacggt acaggtgaaa aaccgattga tgtgaagatt
    14281 gggctttccc ttgatggggg catgagccct gccgactggc gtcacgacgt caacccgcgc
    14341 ggcaaatata tgccaggtaa accagcgtgg tatatgtacg atagctgcca gagcaaacgc
    14401 agcgacagta tcggtgttct ctgctctgcg gtcttctggt cacaaaacaa tggcctgcaa
    14461 ctgcaaaacc tgaccatcga aaacacgctg ggcgatagcg tagatgcagg taaccatccg
    14521 gcggtggcac tgcgtactga tggtgaccag gtacagatta acaacgttaa cattctcggt
    14581 cgtcagaaca ccttctttgt caccaacagc ggtgtgcaga accgtctgga aacgaatcgt
    14641 cagccgcgta cgctggtgac caacagctac attgaagggg atgtggatat cgtttctggt
    14701 cgcggcgcag tggtgttcga taacaccgaa ttccgcgtgg tgaactcacg tactcagcaa
    14761 gaagcgtatg tgtttgcacc ggctacgctg tccaacattt actacggttt cctcgccgta
    14821 aacagccgtt tcaatgcttt cggtgatggt gtggcgcaac tgggccgctc gctggatgtt
    14881 gatgccaata ccaacggtca ggtggtgatc cgtgatagcg ccatcaacga aggttttaac
    14941 acggctaaac cgtgggccga tgcggtgatc tctaatcgtc cgtttgcggg taataccggc
    15001 agcgtagatg ataacgacga aatacagcgc aatctgaatg acactaacta caaccgcatg
    15061 tgggaataca ataaccgcgg cgtgggtagt aaagtggttg cagaggcgaa gaagtaagag
    15121 caattaacta tttgccggat gcggcgtaaa cgccttatcc ggcctacggt tcgatgcgat
    15181 ttgtaggtcg gataagatgc gcaagcatcg catccgacaa taagtgccga atgcgaccta
    15241 cattcacatg gcgcttttta catctgacgg tttttattga agttaatcaa actacccgcc
    15301 ttgataatct cgcgctcttc agcagtcaga ctttccatat agagcgtaat ttccgttacc
    15361 ggcgcatctt catggatcac ataaccttta aacgtcgtac ccggattatc cagcgccgct
    15421 ttaatgccag ggatgtaaat gtaatccccc acttcaaagg ttggcacttc cgccatctgc
    15481 agcggtaaca tcccccagtt gatgacgtta gaacggtagc gtttagtcgc gtactcttcg
    15541 gcaatattcg ccagaccgcc aatcacacgc tggcagctcg ccgcctgttc acgcgcagaa
    15601 ccatcgcctg gtttcaccgc atataccatg ctgccaattt cagtttgcag cggatcaata
    15661 tgctcctgac cagcaatctg cttaatgcgc gcaaacacct ctgtcagctc gctgacattc
    15721 cccgccagac gctgattttc cagctcagca gtcgctttac ttctgccaac ataaccggga
    15781 tcgcggcgtg acagggtaaa ctccgccaga ccaatcggat ttgaacgata agaagaggtt
    15841 tcaccggaag gaatcagttc gtcggtggtg gtcacttcgt cgaggatctt cgagcacact
    15901 ttcaggacga tattgtcagt cagcgcaccc aattccggcc agtctttaat gttcggtccg
    15961 taaatcagcg gttgctgagt tgcccctttc acaaagccct gataaacacg gtttttatac
    16021 ggcgttacat cgaaggcgta ctccggcacg ttgtcccagc aatcaagttc gctggcagag
    16081 gttaaatagc caccgtttgc cgcagtcgca gcgatagaac gagcgtccat caacgccacc
    16141 gctgacatct gcccattagc tggcttagag ccttcgcggt tcgggaagtt acgcgtggtg
    16201 tggcgaatac ttaatccgtt gttgattggc gtatcgcccg cgccaaagca tgggccgcag
    16261 aacgcggttc tgatgattgc gcctgcgcca atcaaatctg ctaccacacc ttttttggct
    16321 aaatccataa acaccggctg tgatgacggg taaaccgcca gcgagaaggt gtcattgcca
    16381 caggattgac cgcgcagtgc attcgccgcc gcgatgacgt tttcgtagtt accgccagaa
    16441 cagcccgcga taatcccctg ctgcactttc aggcgaccat tttccacttt atccagcagc
    16501 gagagtttgg ctttaccgtg cgccacgcgt tcggactcaa tttcaatctc acgcagaatg
    16561 tcggtcaggt tctggttcag tgtgtcgatt tcatacacgt tgctcgggtg gaacggcagc
    16621 gcaatcattg gtttgatggc gcttaaatca acgctgatgc agccatcgta gtacgccatc
    16681 ggttgagggt taagctggca gtaatcctgg ccgcgaccgt gcagcgccag ccagttatgc
    16741 acttcttcat cagtttgcca gacggagctt aaacaggtgg tttccgtggt catcacgtca
    16801 acgctgttac ggaaatcggt agagagcgcg ctaacgcccg gtccaacgaa ctccatgact
    16861 ttgtttttga cgtaaccgtt tttgaacacc gcgccaatga tagccagcgc cacatcctgt
    16921 ggccccacat acggcgcagg ttttccggtc agatgcaccg caaccacgcc cggatagtcg
    16981 atatcccagg tgtcattaag cagctgtttt accaactcac cgccgccctc accgactgcc
    17041 attgtcccta atgcaccgta acgggtgtgg ctgtctgacc cgaggatcat tttgcccccg
    17101 cctgccatca tctcacgcat atattgatgg atgatcgcaa tatgcggagg cacaaaaata
    17161 ccgccataac gctgagctgc cgataaacca aaaacatggt catcaccgtt aatggtgccg
    17221 ccgacggcgc agagtgagtt atggcagttg gtcagcacat acggcagcgg gaaacgttcc
    17281 attccggacg ctttagcggt ctgtacaata ccaacaaagg taatatcgtg agaggctaat
    17341 gaatcaaact taattttaag tttatccata tttccggacg tattatgaga agagagaata
    17401 gaccaggcaa tagtgccttt tttggcttct tcttttttaa tttcgccggt gaaatgttct
    17461 tcggcaatta tttcgttatt actggcgaga aacacgcctt tt
//
LOCUS       DADLFA010000079        17495 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000079 DADLFA010000000
VERSION     DADLFA010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17495
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.079"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..642)
                     /locus_tag="JQH65_002234"
     CDS             complement(<1..642)
                     /locus_tag="JQH65_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAY6139632.1"
                     /translation="MSLNFTVIVWLRLSIGGVYELAFCRSSLKSKKYFIILLALAAIA
                     GLGTHAAWSSNGLPRIDNKTLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKG
                     TQDARELGNAFSADIPDFDLYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAI
                     KDLQSKAPDKNIVIFTHNHCLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDG"
     gene            893..2032
                     /gene="arnB"
                     /locus_tag="JQH65_002235"
     CDS             893..2032
                     /gene="arnB"
                     /locus_tag="JQH65_002235"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HAY6139633.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            2036..3004
                     /gene="arnC"
                     /locus_tag="JQH65_002236"
     CDS             2036..3004
                     /gene="arnC"
                     /locus_tag="JQH65_002236"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HAY6139634.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            3004..4986
                     /gene="arnA"
                     /locus_tag="JQH65_002237"
     CDS             3004..4986
                     /gene="arnA"
                     /locus_tag="JQH65_002237"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HAY6139635.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VAHLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHATRQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISIAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4983..5873
                     /locus_tag="JQH65_002238"
     CDS             4983..5873
                     /locus_tag="JQH65_002238"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HAY6139636.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGTPGTAQIPVTLPTWDEVIGRDVKTEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5873..7525
                     /gene="arnT"
                     /locus_tag="JQH65_002239"
     CDS             5873..7525
                     /gene="arnT"
                     /locus_tag="JQH65_002239"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HAY6139637.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            7522..7857
                     /gene="arnE"
                     /locus_tag="JQH65_002240"
     CDS             7522..7857
                     /gene="arnE"
                     /locus_tag="JQH65_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HAY6139638.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7857..8243
                     /gene="arnF"
                     /locus_tag="JQH65_002241"
     CDS             7857..8243
                     /gene="arnF"
                     /locus_tag="JQH65_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HAY6139639.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(8237..8503)
                     /gene="pmrD"
                     /locus_tag="JQH65_002242"
     CDS             complement(8237..8503)
                     /gene="pmrD"
                     /locus_tag="JQH65_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HAY6139640.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(8613..9968)
                     /gene="menE"
                     /locus_tag="JQH65_002243"
     CDS             complement(8613..9968)
                     /gene="menE"
                     /locus_tag="JQH65_002243"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HAY6139641.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQY
                     ALVLEGENTFPALTSLHIQMVEGTHSALWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLLFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9965..10927)
                     /gene="menC"
                     /locus_tag="JQH65_002244"
     CDS             complement(9965..10927)
                     /gene="menC"
                     /locus_tag="JQH65_002244"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HAY6139642.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELLQMPSIAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALRLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(10927..11784)
                     /gene="menB"
                     /locus_tag="JQH65_002245"
     CDS             complement(10927..11784)
                     /gene="menB"
                     /locus_tag="JQH65_002245"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HAY6139643.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(11799..12557)
                     /gene="menH"
                     /locus_tag="JQH65_002246"
     CDS             complement(11799..12557)
                     /gene="menH"
                     /locus_tag="JQH65_002246"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HAY6139644.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(12554..14224)
                     /gene="menD"
                     /locus_tag="JQH65_002247"
     CDS             complement(12554..14224)
                     /gene="menD"
                     /locus_tag="JQH65_002247"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HAY6139645.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPSLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(14313..15608)
                     /gene="menF"
                     /locus_tag="JQH65_002248"
     CDS             complement(14313..15608)
                     /gene="menF"
                     /locus_tag="JQH65_002248"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HAY6139646.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLEEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(15687..15992)
                     /gene="elaB"
                     /locus_tag="JQH65_002249"
     CDS             complement(15687..15992)
                     /gene="elaB"
                     /locus_tag="JQH65_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HAY6139647.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQVSDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(16047..16508)
                     /locus_tag="JQH65_002250"
     CDS             complement(16047..16508)
                     /locus_tag="JQH65_002250"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY6139648.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLDT
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            16573..17490
                     /gene="rbn"
                     /locus_tag="JQH65_002251"
     CDS             16573..17490
                     /gene="rbn"
                     /locus_tag="JQH65_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HAY6139649.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
BASE COUNT         4114 a         4697 c         4437 g         4247 t
ORIGIN      
        1 cccatccaga taaactttgc ctttttccac atgcatgact aaaccatcca gataatcagg
       61 tttaaatgtc gcatcacgct tatttttagc aatatatgtc aggcaatgat tatgggtgaa
      121 aataacgata tttttatcag gcgctttgct ttgtaagtcc ttaattgcac tataaatctc
      181 attaccgcac tgaagaagtc gtttatctac cgtcagcttt ttacccgcgg aaaaccaggt
      241 cgccgactga atggtccgga cggtattact ggaataaaga tcgaaatcag ggatgtcagc
      301 gctaaaagcg ttgcccagtt cacgggcatc ctgggtacct ttaaccgtaa tacctgtttt
      361 atctgacaag cattgattgg ttgaacgatc gcaacgttca gcatggcgaa acaaaacgac
      421 aaccgggtgc tgctgtgcca gtctggccaa tgttttattg tcgatacgtg gcaaaccatt
      481 gctactccag gcggcatgag tacccagtcc ggcaattgca gcgagcgcca gtaaaatgat
      541 gaaatatttt tttgacttca atgatgagcg gcaaaaagct aactcgtaaa ctccacctat
      601 agacaagcgc aaccagacaa ttaccgtgaa attgagcgac atttctggcg ataattagca
      661 gttggtgtaa tattaaaaat cctacgatgt cggcaaaatg cctcaaaatt ttgccaaatg
      721 caaagcctaa ataagaaaaa atataaaaat ttcaatattt acgtctaata ttagtttctt
      781 aaggttaagt taatattcta tccttaaaat ttcgttccaa atggcaaaat atacacaaca
      841 ctctttatag caaatataag tggacaggta ttcaatggcg gaaggaaaag caatgtcaga
      901 atttttgcct ttttcgcgac cagcaatggg cgtggaggaa ctcgctgcag ttaaagaagt
      961 tctcgaatcc ggttggatca caaccggtcc gaagaatcag gcgcttgagc aagccttttg
     1021 ccagttgacg ggaaatcagc atgccatcgc ggtcagttca gccaccgccg gaatgcatat
     1081 cacgctaatg gcgttggaaa ttggcaaggg cgatgaagtg attacgcctt ccctgacctg
     1141 ggtttcaacc ctcaatatga tttccttgtt gggtgcaacg ccggtaatgg tggatgtcga
     1201 ccgcgatacg ctgatggtca cgcctgaagc gatcgagtca gccattacgc cacgcactaa
     1261 agccatcatt ccggtgcatt atgccggtgc gccagcagat attgacgcca ttcgcgccat
     1321 tggcgaacgt tacggcatcg cagttatcga agatgctgcc catgccgtcg gtacgtatta
     1381 caaagggcga catattggcg caaaaggtac cgctattttt tcatttcatg ccattaaaaa
     1441 tattacctgt gcggaaggtg gcctgattgt aactgataat gaaaaccttg cccgccagct
     1501 acggatgctg aaatttcacg gcctgggtgt cgatgcctat gacagacaaa cctggggccg
     1561 agcaccgcag gctgaagtct taacaccggg ctataagtac aatctgaccg atattaacgc
     1621 cgcaattgcc ctgacacagt tagcaaaatt agagcacctc aacacccgtc ggcgcgaaat
     1681 tgcccagcaa tatcagcaag cactggctgc tctccccttt cagccattaa gccttcccgc
     1741 ctggccgcac gttcacgcct ggcatctgtt tattattcgt gtcgatgaac aacgttgtgg
     1801 tatcagtcgc gatgcgttga tggaagcgtt aaaagaaaga ggcattggta ccgggttaca
     1861 tttccgcgcc gctcacacac aaaaatatta tcgcgagcgt tttcccacgc tgtcgttacc
     1921 gaataccgaa tggaatagcg aacgcatctg ttctttgccg ctgttcccgg atatgactac
     1981 cgccgatgcc gaccgtgtca tcacagccct tcagcaactc gcaggacaat aagccatgtt
     2041 tgaaatccac cctgttaaga aagtctcggt ggttattccc gtttataacg agcaggaaag
     2101 cttaccggaa ttaatcaggc gcaccaccac agcctgtgaa tcgttgggga aagagtatga
     2161 gatcctgctg attgatgacg gcagtagcga taattccgcg catatgctgg tcgaagcctc
     2221 acaagcggag aacagccata ttgtgtctat tttgcttaac cgcaattacg ggcaacattc
     2281 agcgattatg gcgggattca gtcacgttac tggcgactta attattaccc ttgatgccga
     2341 tctccagaat ccgccagaag aaatcccccg cctagtggca aaagccgatg aaggttacga
     2401 cgtggtaggg actgtacgcc agaaccgcca ggacagctgg tttcgtaaaa cagcttcgaa
     2461 gatgattaac cggcttattc agcgcaccac cggcaaagcg atgggtgact acggttgtat
     2521 gctgcgcgcc tatcgccgtc atattgtcga tgcgatgttg cactgccatg aacgtagcac
     2581 ctttatcccg attctggcga atatcttcgc ccgccgtgcc attgaaattc cagtacatca
     2641 tgccgagcgt gagtttggtg aatccaaata cagttttatg cgcctgatta atttgatgta
     2701 cgacctggtg acctgcctta ccacaacgcc gctacgtatg ctgagtctgc tcggcagcat
     2761 tattgcgatt ggaggtttta gcattgcggt gctgctggtg attttacgcc tgaccttcgg
     2821 accacaatgg gcggcagaag gcgtctttat gctatttgcc gtgctgttta cttttattgg
     2881 cgctcagttt atcggcatgg gattactcgg tgaatatatc ggcaggatct acaccgatgt
     2941 ccgcgcccgc ccccgctatt ttgttcagca agttatccgt ccatccagca aggaaaatga
     3001 ataatgaaaa ccgtcgtttt tgcctaccac gatatgggat gcctcggtat tgaagctctg
     3061 ctggctgccg gttacgaaat tagcgccatt tttacccata ccgataatcc cggtgaaaaa
     3121 gccttttatg gttcggtggc tcatctggcg gcggaaagag gcattccggt ttatgcgccg
     3181 gataacgtta atcatccgct gtgggtggaa cgcattgccc aactgtcacc agaggtgatt
     3241 ttctcttttt attatcgcca tcttatttgc gacgagattt tgcagctcgc tcccgcaggt
     3301 gcatttaatc tgcacggttc gctgttacca aaatatcgtg gtcgcgcgcc gctgaactgg
     3361 gtgctggtga acggtgaaac ggaaacgggc gttacattgc accgaatggt aaaacgtgcc
     3421 gatgctgggg ccattgtggc gcaactgcgc gttgccattg cgccagacga tatcgctatt
     3481 acgctgcatc ataaattgtg ccatgccacg cgccagctac tggaacagac attacccgcc
     3541 attaaacacg gtaatattct ggaaatcgcc cagcgcgaaa acgaagccac ctgttttggt
     3601 cgcagaacgc cggatgacag tttccttgaa tggcataaac cggcatccgt actgcacaac
     3661 atggtacgtg ccgttgccga tccgtggccg ggtgccttca gctatgttgg caatcagaaa
     3721 ttcaccgtct ggtcgtcgcg tgttcatcct catgccagca aagcacagcc ggggagcgtg
     3781 atttctattg caccgctgtt gattgcctgt ggcgatggcg cgctggaaat cgtcaccgga
     3841 caggcgggcg acggcattac tatgcagggc tcgcaattag cgcagacgct gggcctggtg
     3901 caaggttcac gcttgaatag ccagcctgcc tgcaccgccc gacgccgtac ccgggtactc
     3961 atcctcgggg tgaatggctt tattggcaac catctgacag aacgcctgct gcgcgaagat
     4021 cattatgaag tttacggtct ggatattggc agcgatgcga taagccgttt tctgaatcat
     4081 ccgcattttc actttgttga aggcgatatc agtattcatt ccgaatggat tgagtatcat
     4141 gtcaaaaaat gtgatgtcgt cttgccgctg gtggcgatag ccacgccgat tgaatatacc
     4201 cgcaacccgc tgcgcgtatt tgaactcgat tttgaagaga atctgcgcat tatccgctac
     4261 tgcgtgaagt accgtaagcg aatcatcttc ccgtcaactt cagaagttta tgggatgtgt
     4321 agcgataaat acttcgatga ggaccattct aatttaatcg tcggcccggt gaataaacca
     4381 cgctggattt attcggtatc aaaacaatta cttgatcggg tgatctgggc ctatggcgaa
     4441 aaagagggtt tacagttcac cctcttccgc ccgtttaact ggatgggacc acgactggat
     4501 aaccttaatg cggcacgaat cggcagctcc cgcgctatta cgcaactcat tctcaatctg
     4561 gtagaaggtt caccgattaa gctgattgat ggcggaaaac aaaaacgctg ctttactgat
     4621 attcgcgatg gtatcgaggc gttataccgc attatcgaaa atgcgggaaa tcgctgcgac
     4681 ggtgaaatta tcaacattgg caatcctgag aacgaagcga gcattgagga actgggcgag
     4741 atgctgctgg cgagcttcga aaaacatccg ctgcgccatc atttcccacc gtttgcgggc
     4801 tttcgcgttg tcgaaagtag cagctactac ggcaaaggat atcaggacgt agagcatcgt
     4861 aaaccgagca tccgcaatgc ccaccgctgc ctggactggg agccgaaaat tgatatgcag
     4921 gaaaccatcg acgaaacgct ggatttcttc ctgcgcaccg ttgatcttac ggataaacca
     4981 tcatgaccaa agtaggctta cgcattgatg tcgatacctt tcgtggcacc cgtgaaggcg
     5041 tgccgcgtct gctggaaatc ttgagtaagc ataatattca ggccagcatt tttttcagcg
     5101 tcggcccgga caatatgggc cgccatctct ggcgactggt gaagccacag tttttgtgga
     5161 agatgctgcg ctcaaacgcg gcatcgcttt atggctggga tattttactg gcaggtacgg
     5221 cctggccagg taaagagatt ggtcatgcca atgccgatat cattcgtgaa gcggctaaac
     5281 atcacgaagt cggcctgcac gcctgggatc accatgcctg gcaagcccgt agcggtaact
     5341 gggatcggca aacaatgatc gacgatattg cacgcggtct tcgcactctg gaagagatta
     5401 tcggtcaacc ggtaacctgt tctgccgctg cgggctggcg tgccgaccag aaggtgatcg
     5461 aagcaaaaga agcgttccat ttgcgctaca acagcgattg tcgtggggcc atgccgttcc
     5521 gtccattgct cgaatcagga acccctggca ctgcgcaaat tccggtgacc ttacccacct
     5581 gggatgaagt gattggtcgg gatgtgaaaa cagaagattt taacggttgg ttactcaacc
     5641 gcatcctgcg agataaaggc acgccggttt ataccattca tgcagaagtt gaaggctgcg
     5701 cttatcagca taattttgtg gatctcctca aacgcgcagc tcaggaaggc gtgacatttt
     5761 gccctttaag cgaactgtta tcagagacgt tgccgctcgg acaagttgtt cgcggaaata
     5821 ttgccggacg tgaaggctgg ctgggttgcc aacaaattgc gggtagtcgc tgatgaaatc
     5881 ggtacgttac cttatcggcc tcttcgcgtt tattgcctgc tattacctgt taccgatcag
     5941 cacgcgtctg ctctggcaac ccgatgaaac gcgttatgcg gaaatcagtc gagaaatgct
     6001 ggcatccggc gactggattg tgccccatct gttagggcta cgttatttcg aaaaacccat
     6061 tgccggatac tggattaaca gcattgggca atggctattt ggcgcgaata actttggtgt
     6121 gcgggcaggc gttatctttg cgaccctgtt aactgccgcg ctggtgacct ggtttactct
     6181 gcgcttatgg cgcgataaac gtctggctct actcgccaca gtaatttatc tctcattgtt
     6241 tattgtctat gccatcggca cttatgccgt gctcgatccg tttattgcat tctggctggt
     6301 ggcgggaatg tgcagcttct ggctggcaat gcaggcacag acgtggaaag gcaaaagcgc
     6361 aggattttta ctgctgggaa tcacctgcgg catgggggtg atgaccaaag gttttctcgc
     6421 ccttgccgtg ccggtattaa gcgtgctgcc atgggtagcg acgcaaaaac gctggaaaga
     6481 tctctttatt tacggctggc tggcggttat cagttgcgta ctgacggttc tcccttgggg
     6541 actggcgata gcgcagcggg agcctgactt ctggcactat tttttctggg ttgagcatat
     6601 tcaacgcttt gcactggatg atgcccaaca tagagctccg ttctggtact acgtgccggt
     6661 catcattgcc ggtagcctgc cgtggctggg attactcccc ggtgcactgt acacaggctg
     6721 gaaaaaccgc aagcattccg caaccgtcta tttgttgagc tggacgataa tgccgctgct
     6781 gtttttctcc gtcgctaaag gtaaattgcc cacctatatt ctttcctgct ttgcatctct
     6841 ggcaatgctg atggcgcatt acgctttgct ggcagcaaaa aataatcctc tggcgctgcg
     6901 gattaatggc tggattaaca tcgcttttgg cgtcactggc attattgcca catttgtggt
     6961 ctccccgtgg ggaccaatga acacgccggt gtggcaaacc ttcgagagct ataaagtctt
     7021 ttgtgcctgg tcgatttttt cgctatgggc atttttcggc tggtacacct taacaaacgt
     7081 cgaaaagacc tggtctttcg ccgcgctttg cccgctgggg ctggcgttgc tggtaggatt
     7141 ttcaattcct gacagagtca tggaaggaaa acatccgcaa ttttttgtcg agatgacaca
     7201 agaatcactt caaccaagcc gctatattct tactgatagc gtcggtgttg ccgcaggtct
     7261 ggcatggagc ctgcaacgcg atgacatcat catgtatcgc cagacaggtg agttgaaata
     7321 cggccttaat tatccggatg cgaaagggag atttgtcagc ggtgatgagt tcgcaaactg
     7381 gcttaatcaa catcgtcagg aggggattat tacactcgtg ctttcggttg accgcgatga
     7441 agatatcaac agtctcgcca ttccgcccgc agatgccatc gatcgtcagg agcgtctggt
     7501 gctgattcag tatcgtccca aatgatctgg ctaacattag tctttgccag cttgcttagc
     7561 gttgccgggc agttgtgtca gaaacaggca acctgctttg tggcgataaa caaacggcgc
     7621 aaacatatcg tgctgtggct gggactggcg ctggcttgtc ttggtcttgc catggtgctc
     7681 tggctgctgg tcttgcagaa cgtaccggta ggcattgcct acccgatgtt aagtctgaat
     7741 tttgtctggg tgacgctggc tgcagtaaaa ctgtggcacg aaccggtatc gccgcgtcac
     7801 tggtgtgggg tggcgttcat tattggtggc attgtgatcc tcgggagtac ggtgtaatgg
     7861 gcctgatatg gggattattc agcgtcatta ttgcctcagt tgcgcaatta agcctgggtt
     7921 ttgcggcaag tcatctgccg ccgatgacgc acttatggga ttttatcgcg gctctgttag
     7981 cttttggcct ggatgcccgg atactgttac tcgggctgct gggttatctg ctttccgttt
     8041 tttgttggta taaaacgcta cataaacttg cgctaagcaa agcctatgcc ttgctcagta
     8101 tgagttatgt gctggtatgg attgcttcaa tggtacttcc cggctgggaa gggacttttt
     8161 cgctgaaagc actactggga gtagcctgta ttatgagcgg gttgatgctg attttcctgc
     8221 ccacgacaaa acaacgttac tgagttttcc ctgccgctgt acactgccgt tcccactcat
     8281 cggggctata actgcttgcc gagaggactt tcaccgtatg cagcttttca cgattaatac
     8341 aataaagcgc attctgcaaa ggcgagagta aatcccccac tttcacggcg aagtcactct
     8401 tcacttcggc gatcattttt atcgcgccgc cagcatcgca gagcataagt acatgtctgt
     8461 tatcttgttt attgcagcac gattttttga ccagccattc cattgcatta tcctgtttgc
     8521 taagagtttt cacatcaatt tacagattaa cagataaact ctttatcaac ggcaatggaa
     8581 tggcagaaat gagaagtgaa acggagggtt aattattgct gacgttgcac ccactctttt
     8641 agcgcctgac gtgaaatttt aataccgccg tttttcagct ccggcggtaa agtcagccag
     8701 cgcaccggtt gttgaaaacg agccagctta tctttcaccc attcgccaag atcaacggtt
     8761 tgctggtcat actccaccac cgctaccggc cgatgaccaa actccttgtc ggcaacggga
     8821 acgataaaca cctgcaaaac cgcaggatgt gcagcaataa cacgctcgac ttcttccggc
     8881 tgaataccct caccgccact gaataacaga ttgtctaaac gtccgacaat ggtcagcttg
     8941 ccattatgca tctcaccgcg atcgcgcgta gcgtaccagc cttcgtcatt aaccagtgaa
     9001 accagttgcc cgttacgcca gtaaccttct gccatactgg cagcccgcag ccacacttca
     9061 ttattaacga ttttcacttc ccgacccggc agcggcgaac caacgtctgc caggccgtcg
     9121 gcttctttcg cacataccgt ggaggcaaac tcggtcagac catagccgca aaagcaacga
     9181 atcccctgct cgcgcgcctg ttccgtcaac tcgaccggga tagccgcgcc gccaagtaac
     9241 accgctttca gggaaacgga actgcggtta accagcaaac gccagagttg tgttggcacc
     9301 agtgaagcgt gagtacagcc tgccagcatt tgctccaatg gctgtttatc acgcaccgtc
     9361 atccgcgcac cagcgtataa ccagcgccac ataattccct gaccggagac gtgaaacagc
     9421 ggtaaagaga gcaaccagtc atcgtgatcg ccaaacggaa tcagcgataa cacaccttgc
     9481 gcactggcaa gatgggcctg ataagtatgg acagcggctt tcggtaaacc ggtggagccg
     9541 gaggtcaatg tcattgaaca aagacgtgtt ggttgccaca gagctgaatg tgtgccttca
     9601 accatctgaa tatgcagtga agttaacgcc gggaaagtat tttctccttc cagcaccagc
     9661 gcatattgca gtgtaagatt tggcagtaat tcctcaagca acggttgcgg aagctgtgga
     9721 tttacgggca gcacccgcgc cccgcattgc agtaacgcca gccaggcgag cagcgtttgc
     9781 ggcgtattcc acgcccgcaa catcacgccg ctgccctcaa ccacaccctg cgccgcaaag
     9841 cccgtagcta actcatcgac gcgagcacaa agctcgcgcc agttgagttg ctcgtcatta
     9901 agacgtaagg cgatggcttc tccccgcact tgccgccagt gacgccacgg ccagtcagag
     9961 aagatcataa caaccgctcc agtgcatcaa cttccacgac aggcagcgtg ctacccggcc
    10021 agcgacgtac ctgctgcgcc tgcatcagat ccagcgtgtc cagccctgga atggtgtccg
    10081 gcgttaacca ggcggcaatc cgcgccagtt gcgttaagcc taagctcgac tcaatagaag
    10141 aactgatcac cgccgtcagc ctcaacgcgt gcgccgcctg tacctgctcg cgtacttttt
    10201 ccagactgcc cgtgagcgtg ggtttgataa ctaccgcgcg cacgccctct tcagccacaa
    10261 aggcaaaatc cggctcgcgc aggctttcat cccaggcaat ggcaatgccg gtttcacggg
    10321 caaacgctcg cgaatcatcg cgggttttgc acggctcttc gagaaacgcg atgcggtggc
    10381 gataatccgg gttaacatat ttggcaaact gctgaccttt cagcggtgtc caggcgcgat
    10441 ttgcgtcaag acgcaaatgc agatccggaa ttgcctccag caacagatta accaccatgc
    10501 cgtcgcgcac ggcttcgtac aatcccactt tgaccttcgc cactttctcg cctggcatat
    10561 cagcaagttt gaggatcaga tcgtcaggat cgccattaca cagtggtgcc gcacggtaat
    10621 tggccgcctg cgggagcgta tctgccagct ccgccaatgc acagcttacg ccaaaggcaa
    10681 tggaaggcat ctgcagtagc tcgcaatcgc ctgccagcca gttatttacc caggcaagca
    10741 gcacgctttg cgcctcttcc caggtttcct ggctgaagcc cggcagtggg gagatctccc
    10801 cccacccttc gcgctcgccc tcacgcaggc aaacgtacag cccgtcgcgg gtttttaacc
    10861 gcctgtcgcg cagaaccacc cccgcgtcca tggggatctg ccagcggtat acctgcgcgc
    10921 tacgcattac ggattccgtt taaatttgct gaaatcaggc tgacgtttct ggttgaaagc
    10981 gttgcgacct tcctgacctt cttccgtcat gtagaacagc atggtggcgt tgcccgccag
    11041 ctcctgcagc cccgcctgcc cgtcacagtc ggcgttcagt gcagctttca ggcagcgcag
    11101 cgccatcgga ctgttttgca gcatttcgcg gcaccagcga acggtctctt tttccagatc
    11161 cgccagcggt accacggtgt tgacaaggcc catatccagc gcctgtttcg cgtcgtactg
    11221 acggcacagg aaccagattt cccgcgcttt tttctgtccg acgatgcgag ccatgtagga
    11281 agcgccccag ccgccgtcga aggaaccgac tttcgggcca gtctgaccga agatggcatt
    11341 atctgccgcg atagtcaggt cgcacatcat gtgcagaacg tgaccgccgc cgatggagta
    11401 gccagccacc atcgcgacaa ccggtttcgg acaggtacgg atctgacgct ggaagtccag
    11461 cacgttcagg tgatgtacgc cggaatcatc tttatagccg ccgtaatcac cacgcacttt
    11521 ctggtcaccg ccggagcaga acgctttatc acctgcgcca gtcagaatga tcacgccgac
    11581 gttgtcgtca taacgcgcat ctgccagtgc ctggatcatc tcttttaccg tcagaggacg
    11641 gaaggcattg cgcacctgcg gacgattaat ggtgattttt gcgataccat cggtggattt
    11701 ttcataacga atgtcctcga aaccttcgga gcagtcgtgc cattcaaccg gtgcgtaaag
    11761 cattgcttca tcaggataaa tcatattgtg tcctttagtc agaaacgcaa gatctgcgcc
    11821 agacttgcga ttacgccagc gggattttcc cgatgcgcgt tatgtccggc gcgaggaatg
    11881 acatggcagt cggcagccag ttccgccgcc agggcgcgga atttgctgtc acgttcacca
    11941 cataaataat aaaacgcaaa tgtgcgggcg ctaaggttag cacgtaaatc aggctggacg
    12001 gcgagagaag tcgcctccag catggcggca agggttgcgc cattattgtt gctgcgcagc
    12061 gccaccagct cccggcgttg atcgtcattg agcgaggcaa aaacaggctg ttgataccag
    12121 tcggcaaata ccgccgttaa cggttctgtg cgaaaacgct gcgcccattg gcgatcggaa
    12181 cgctgacgtt ccgcacgttg ttcagcattt tgcagccccg gatgcccgcc ttcgacaata
    12241 accccacaaa gccccgccag cccctggcaa gccgccatca tcgccacccg tccgccaagc
    12301 gagtacccca ccagccagaa gtcaaggatg ttgtaactaa ccaaggtttt acgcagtaag
    12361 tcggtgacat catcaaatcc atcgacgcta atcgccgccg aaccaccatg acctgggaga
    12421 tcaacataca accgtgagta gtcggcaaac gcctcgccca cttcttgcca ttcgtggcaa
    12481 tcgccggaaa aaccgtgcaa aaacaccagc cagggtaaac ctggttttcc gtgttttgcc
    12541 tgcgcgtgca ggatcataaa tggcttacct gcgccagaag ttgctggagc gtttgcgcac
    12601 catcggtgtc gttaaccacc atttcaatca ccgtggtggt tggcgtgcgc caggcgtcgg
    12661 caaatgccgt ttcaagttcc tgccagtttt gcggacgatg atatttcagc tcgaacatcg
    12721 cggcggcgtg ctcaaaatgg acgttttgcg gcatcagata gaaacgctca cgctcgcttt
    12781 gcggcgttgg caacagcgag aaaatttgcc cgccgttgtt gttcaccaca attaatacca
    12841 gcggcgcaga aacctgacgc aataacgcca gcgcgttgag atcgtaaagt gcggagagat
    12901 cgcccacaat cgccagcgtc ggtttgccgc ttgcccgctg aacgcctgcg gcggtcgaaa
    12961 gcagtccgtc gataccgctg gcccctcggt tgctgtacac cgggtaacct gccggaagtt
    13021 gcgaaagcgc atcaatcaga cgtaccacca ggctgttacc gacaaacaat tgcccctgtt
    13081 caggcagata gtcgctgatg cgatgcgcca gttgcgcttc gccaaacgca tcgcgacggg
    13141 caataaccgc ctgcattgcc tgttccgcca ggcgcgggat ttcaacgcac cagggctggc
    13201 gtttttctgc cggatgcagc tccagccagt cggcaatatt ggcaattaag cggcgtccac
    13261 gatggtgcgc cggatcaagc cgcccttcga tgtcatccac aatccagtac tcttcgggtt
    13321 cacagcttgc ctgccattgc aggagccgtt tgcccgtcag gctgcttccc agttgcacca
    13381 caatttgcgc ctgctgcagc tcgctggtcg ctttggcatt gcctaaccaa agatcggcac
    13441 acggcagcgg ctgcccggtt tgtgacagca catcgccaat cagcggccag ccaagagttt
    13501 gcgcccacag ggcaactttt ttgccctctt ccgcactcat gcgcccggca accaccacgc
    13561 cgcgcttttg tcgccagaag aaccagtcgc gctgtttttc actttccaga ctaggcgctt
    13621 cacgcagcca cggtttgtcg tcctgccacc agtcacccag acgctgttgc cagctaagcc
    13681 cggtatcgtc catttcgcca tacagcggtt cagcaaacgg gcagttgata tggactcccc
    13741 ccgcatgaag cgtaccgaga gcgtggtcga tggtagaaac cagccaacgt gcggggatat
    13801 cctgggtcgg gcgcggcaat gaaatactgt gcgtggggtg agaggcgaac attcccggct
    13861 ggcgaattgc ctgattcgcg ccgcagtcaa ttagctccgg cgggcgatcg gcggttaaga
    13921 gaatcagttt ttctccggtt aacccggctt caatcagtgc cggatagaga tttgccaccg
    13981 cagtgccgga ggtcacaatc accgccaccg gctgcttgct gactttcgcc agccccagcg
    14041 ccagatgccc caacccacgc tcatcgaaat gggtgtggtg aatgaatgcg gaattctccg
    14101 ccgccgctaa cgttaacggt gtagaacgcg agcctggggc gatacagatg tgtctgacgc
    14161 cgtgacgcgt taatgcttcc agaatgaccg ccgcccagcg tcggttaaat gcgcttactg
    14221 acatgagttt gtccggtatc aatattgcgg ctaagtataa ggagctacaa aaatagatta
    14281 ttgatatgaa tcggtaatga tgcgactcat tactattcca tttgtaataa agtacgcaac
    14341 cctgccgctt tgttgtcgat ttcctgccac tcttgctcgg ggtcggaacc acggacaatg
    14401 cccgcgccag catataatcg cacgacattg ccgctaattt ttgctgagcg cagggaaacg
    14461 cagaattcgc tttgttgtaa tgagagatag cccgcagaac cggcgtacca ttctcgggtg
    14521 aacggttcgt gacgggcgat aaactgtcgc gccagatcgc gcggtaagcc agcaactgct
    14581 gccgttggct gcaactgatg taaacagatc acatcatccg ctttgttgag tgaagtccag
    14641 atacagcggc gaagatgctg cactttacgc agacgcagta cctgcggcgg taaaacatcc
    14701 agcgtctggg tatcggcctg taatcgttgg cagatatctt ccaccaccag catgttctcg
    14761 cgctggtttt tatcatccgc catcagccac tcttctaact gctgcgcctg cttatcatca
    14821 ggatgatttg ctactgttcc cgccagcgct tcagtacgca gcgctttgtc acgccgccgc
    14881 cataaccgtt ccggtgaaga gccaagaaaa gcattttcgc catcaaaggc catgtaaaaa
    14941 tggtagcaat tcagattcag tcgacgactg gcagccatca tcgccgccgc gttgaccgga
    15001 cttgcgaaat gcaggtcagt tgcccgagcg agcaccactt tgtcgagctc accttcggcg
    15061 atggttttcg ttgccagttc gattaattgc gtccagcccg ttttgtccgg ccagtgttgt
    15121 tctcgcgtgg tggttaaatg taacccaggc aagggcttga tactcaccag tgtggcgata
    15181 aattcttttg cctgaatcgc atcgtgctga agggagcttt cgctgaatag cgtcagccgc
    15241 agcgtggctt taccgccaca gcgtcgccat tccaggcggg gtaaaagtaa attgccctgc
    15301 gacgggtcaa aagcattcag cccccaaatg cgtaagtcgg cgtgttccgg gtgctggcga
    15361 agaaaacgtt gtgcctggtc caacgacgta aaacgggtaa tcgcgcccag gacggcagct
    15421 tcttcatcac cattacgttg ttgccagtag aattgcgggt acgtttgctg actggccagc
    15481 cagctcaagg catcaaaagc gtctttgaga gggaaaggaa tatcgataac ccgaatgccg
    15541 ggtgtcgccg gaatctcttg cgacaaatgg cgcagtagat tttccagcgc cgtagtaagt
    15601 gattgcacgc ggacctctcc ctgttaaaag cctcacatta tacggggtac tacaaaaaaa
    15661 atgcagtacc ccggtgtagg gaggttttaa cggcgtgcca gcaacagtcc tagtaccagc
    15721 ccaacggccg cgcccacacc aattccttgc caaggttttt cgtggacgta gtcatcagca
    15781 cgataaacag cctgcttcgc ccgatagtaa taactgtctg aaacctggct aacccgtttt
    15841 ttcacatcat ccagcgcttt ttctgcacgc gctttcagct caacatattt ctgatcagcg
    15901 ggatcgccag aggagcggag cacctcttcc agtgtctcac taagcagcgt caggtcgtca
    15961 tcgatacgtg tatcaccaaa ctgattagac attctcgttc tccatttgcg taaaacctgt
    16021 cactaactat agacaagggt tgttgattac gcctgaatta cctcgcgtgc catcccaatg
    16081 tgtgggatac catcttcttc atagacctcc gtcacaggga taaaaccgaa actctggtag
    16141 aagttttgca gatgcgcctg cgccccgagg taaacaggct tatcaggcca gtgatgcgtg
    16201 caggtgtcga gggttttact catcagttgc tgccccactt tttcgccacg caacgcttca
    16261 ctgacaatca cccgaccaat aacgaccggc tcaagatcat catcactttt cagaatcctc
    16321 gcatatgcca ccagttcatc atttttccaa ccgagaatat ggcgattatc ccccgtcaaa
    16381 tcatcaccat cgatatcctg ataagggcaa ttctgctcga caacaaacac cgcgcaacgc
    16441 agttgtaata aggcataaag ctgggaaacc gataattcag agtgatgcag atcttgccat
    16501 tcaatcataa tgctctcctt ttagatgtat accttatact agccggaata ttttttgaaa
    16561 cgtgatgaac tcatggaatt aattttttta ggtacttcag ccggtgtgcc aacccgcacg
    16621 cgcaatgtca cggcaatatt gcttaacctg caacatccga cccagagcgg actttggttg
    16681 tttgactgcg gtgaaggcac ccagcatcag ctactgcata ccgcctttaa ccctggaaaa
    16741 ctggacaaga tttttatcag tcaccttcat ggcgatcatc tttttggttt acccggcttg
    16801 ctgtgcagtc gttctatgtc aggcattatc caacccttaa cgatttatgg tccgcaaggt
    16861 atccgtgaat ttgtggaaac cgcgctgcgg attagcggct catggaccga ttatccgctg
    16921 gaaattgtcg aaattggcgc tggcgaaatt ctcgatgatg gcctgcgcaa agtaaccgct
    16981 tatccgctgg aacacccact ggaatgttat ggctatcgta ttgaagaaca tgataaaccg
    17041 ggtgcattaa atgcccaggc attaaaagct gctggcgtgc cgcctggccc actgtttcag
    17101 gaattaaaag caggcaaaac aatcacgctg gaagatggaa ggcagattaa cggcgcagat
    17161 tacttagctg ctccagtgcc aggtaaagcg ctcgctattt tcggcgatac cggcccctgc
    17221 gatgccgcac ttgacctggc taaaggtgtc gatgtcatgg tgcacgaagc gacgctggat
    17281 ataaccatgg aagccaaagc caatagtcgc ggccatagct ctacacgcca ggctgcgaca
    17341 ctagcccgtg aggctggagt cggtaagcta atcattaccc acgtcagctc gcgctatgat
    17401 gacaaaggtt gtcagcacct gttacgtgaa tgcaggtcaa ttttcccggc gactgaacta
    17461 gcgaatgatt tcaccgtgtt taacgtttaa ctctg
//
LOCUS       DADLFA010000080        17483 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000080 DADLFA010000000
VERSION     DADLFA010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17483
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.080"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(34..2631)
                     /gene="topA"
                     /locus_tag="JQH65_002252"
     CDS             complement(34..2631)
                     /gene="topA"
                     /locus_tag="JQH65_002252"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAY6139650.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            3011..3262
                     /locus_tag="JQH65_002253"
     CDS             3011..3262
                     /locus_tag="JQH65_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HAY6139651.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(3298..4347)
                     /gene="sohB"
                     /locus_tag="JQH65_002254"
     CDS             complement(3298..4347)
                     /gene="sohB"
                     /locus_tag="JQH65_002254"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HAY6139652.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMGSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKY
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            4567..5325
                     /locus_tag="JQH65_002255"
     CDS             4567..5325
                     /locus_tag="JQH65_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HAY6139653.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDICPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            5322..5913
                     /gene="cobO"
                     /locus_tag="JQH65_002256"
                     /pseudo
     CDS             5322..5913
                     /gene="cobO"
                     /locus_tag="JQH65_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
     gene            complement(5953..6825)
                     /gene="rluB"
                     /locus_tag="JQH65_002257"
     CDS             complement(5953..6825)
                     /gene="rluB"
                     /locus_tag="JQH65_002257"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HAY6139654.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(7036..8931)
                     /locus_tag="JQH65_002258"
     CDS             complement(7036..8931)
                     /locus_tag="JQH65_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HAY6139655.1"
                     /translation="MAGKFRCILLLIAGLFVSSLSYAENPEIPSYEEGISLFDVEATL
                     QPDGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVTRDGIPEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNHWPF
                     EIYKTRFSLKFPDIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESRDPFYREDFTVL
                     YRWPHALLSNAPAPQTTNIFSHLLLPSTSSLLIWFPCLFLVCGWLYLWKRRPQFTPVD
                     VIETDVIPPDYTPGMLRLDAKLVYDDKGFCADIVNLIVKGKIHLEDQYDKNQQILICV
                     NEGATRNNEVLLPAEQLLLEALFRKGDKVVLTGRRNRVLRRAFLRMQKFYLPRKKSLF
                     YRPDTFLQWGGLAILAIILYGNLSPVGWAGMSLVGDMFIMICWIIPFLFCSLELLFAR
                     DDDKPCVNRAIITLFLPLICSGVAFYSLYINVGDVFFYWYMPAGYFSAVCLTGYLTGM
                     GYIFLPKFTQTGQQRYARGEAIVNYLARKEAATHSGRRRKGETRKLDYALLGWAISAN
                     LGREWALRIAPSLSSAIRAPEIARNGVLFSLQTHLSCGANTSLLGRSYSGGGAGGGAG
                     GGGGGGW"
     gene            complement(8959..9579)
                     /locus_tag="JQH65_002259"
     CDS             complement(8959..9579)
                     /locus_tag="JQH65_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAY6139656.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(9576..10457)
                     /gene="yciV"
                     /locus_tag="JQH65_002260"
     CDS             complement(9576..10457)
                     /gene="yciV"
                     /locus_tag="JQH65_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HAY6139657.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLTTLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            10595..10639
                     /gene="trpL"
                     /locus_tag="JQH65_002261"
     CDS             10595..10639
                     /gene="trpL"
                     /locus_tag="JQH65_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HAY6139658.1"
                     /translation="MKAIFVLKAWWRTS"
     gene            10731..12293
                     /gene="trpE"
                     /locus_tag="JQH65_002262"
     CDS             10731..12293
                     /gene="trpE"
                     /locus_tag="JQH65_002262"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HAY6139659.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLTLLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKNTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRTGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGIVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            12293..13888
                     /gene="trpD"
                     /locus_tag="JQH65_002263"
     CDS             12293..13888
                     /gene="trpD"
                     /locus_tag="JQH65_002263"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HAY6139660.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQRKLEPTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            13892..15250
                     /gene="trpCF"
                     /locus_tag="JQH65_002264"
     CDS             13892..15250
                     /gene="trpCF"
                     /locus_tag="JQH65_002264"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HAY6139661.1"
                     /translation="MQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDALQ
                     GARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLPI
                     VSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMGG
                     LTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGIN
                     TYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAGA
                     IYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQLH
                     GNEDQLYIDTLREALPTHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDWS
                     LLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTLR
                     AY"
     gene            15262..16455
                     /gene="trpB"
                     /locus_tag="JQH65_002265"
     CDS             15262..16455
                     /gene="trpB"
                     /locus_tag="JQH65_002265"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HAY6139662.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVCEFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRETPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            16455..17261
                     /gene="trpA"
                     /locus_tag="JQH65_002266"
     CDS             16455..17261
                     /gene="trpA"
                     /locus_tag="JQH65_002266"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HAY6139663.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLRDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
BASE COUNT         4221 a         4670 c         4347 g         4245 t
ORIGIN      
        1 cctttgttta taaaaacctg acagaattaa aggttatttt tttccttcaa cccatttgcc
       61 atcaacataa aatgctgacc agccagtcgc ctttccgtct ttttccgaag agacatattg
      121 ctgtttggtt ttacggctaa agcgaaccat ggtcttatta ccttccggat cctgctgtgg
      181 cgcatcggcc agataacgca gtttttccgg cagacggtcg cggaagcgat aaagctcttc
      241 caccagtggc gcacgcgttt cacgcgattt cgggaaagtg ttggcagcca ggaaaacacc
      301 cgcagcgccg tcacgcagca cgaaataagc atctgatttt tcgcacggca gctcaggtaa
      361 tggcaccgga tcttctttcg gtggtgccac ttcgccgtta cgtaaaatct tacgtgtgtt
      421 tttacactct tcgttggtgc aggccatgta tttaccgaaa cgccccattt tcaggtgcat
      481 ttcagagcca catttttcac actcaacgat cgggccgtca taacctttaa tgcggaattc
      541 gccctcttcg atctcgtaac cgtcgcaggt tgggttatta ccacagacat gcaacttacg
      601 tttcggatcg atgagatagc tgtccatcgc cgtgccgcat ttcgggcaac gacgttttgc
      661 gcgcagcgcg ttggtttcag cgtcttcgcc ttccagtacg ttcagcactt cgttttccgg
      721 caccaggtta atggtggttt tgcaacgctc tttcggcggc agcgcataac cagaacagcc
      781 aaggaatacc ccggtgctcg ctgtgcgaat ccccattttg cgaccacaag tcgggcagtc
      841 aatgctggtc agaaccatct ggttcgggcg cataccaccc tcttccggat ctttttcagc
      901 tttatctaac tgctgggtga aatccgagaa gaagtgatcc agtacagctt tccactctgc
      961 ttcgtgattt gccacctggt cgaggctgtt ttccatctgt gcggtgaagt cgtagttcat
     1021 taactcgcgg aaattttctt caaggcgatc ggtgacgatt tcgcccattt tttccgcata
     1081 gaaacgacga ttttctactc gcacgtagcc acgatcctga atggtcgaaa tgatcgacgc
     1141 ataggtagac ggacgaccga taccgcgttt ttccagctct ttaaccagcg atgcttcact
     1201 gaaacgggct ggcggcttgg taaagtgctg ggctggtgta agttcaacga gcgtcagagc
     1261 atcgccttta ttaactgctg gtaagatgcg atcttcatcg cctttacgca acgcaggcat
     1321 cacttttgtc cagccatcaa aacgcaaaat acgaccgcgt gctttcaggc ggaaatcgcc
     1381 cgcaccaacg gtcagcgtcg tggagtcata tttcgctggg gtcatctggc aggctacgaa
     1441 ctgacgccag attaactggt acagtttctg cgcatctgct tccatatcct tcagcgattc
     1501 cgccatcaca ttgacgtcag aaggacgaat cgcttcgtgc gcttcctgtg agttttcttt
     1561 gctggcgtac tgattcggac tttccggcag atatttctta ccaaaattat cgctgatata
     1621 accgcgaacc atatttaccg cgtcctgact caggttagtg gagtcggtac gcatgtaagt
     1681 gatatagcct gcttcataca aacgctgcgc catcatcatg gtttttttca cgccaaatcc
     1741 aagacgggtg ctggcagctt gttgcagcgt agaggtaata aaaggagcgc caggtttact
     1801 ggttgtcggt ttgtcttcac gttccagcac gctgtagcgc gctttttcca gcagacttac
     1861 cgcagcctga gtttgttctt tgttgaccgg acggaacggt ttgtcgttct gatgagtcac
     1921 ctgtaacgcc aacgcttcac cagatggcgt ggtcgtgctg gcatcgactt cccagaactc
     1981 ttccggcacg aacgctttaa tttcacgctc acgctcgacc accaggcgaa ccgccaccga
     2041 ctgcacacga ccggcagaca gaccacgagc gatctttttc catagcagcg gcgaaaccat
     2101 ataccccacc acgcggtcca taaagcgacg cgcctgctgg gcattaacac gatcaatatt
     2161 cagctcaccc ggtttgttaa atgcctggcg gatcgcgttt ttagtaattt cgttaaacac
     2221 cactcggcta tagcgcgcat catcaccccc aatcacttcc cgcaggtgcc atgcaatggc
     2281 ttccccttcg cggtcaaggt cggttgcgag atagatgtgg tcggcttttt cagccagttg
     2341 tttcagttca gagacgacct tctctttacc aggcaacact tcatagtgcg cctcccaatt
     2401 gtgccacggg tcaaccccca tacggttgac gagagcgcca cgttcatcct ttttaggctt
     2461 tttagccgtc ttggtggagg tagagtcggc actctttttg gcagctgagc cactggtcgg
     2521 caaatcgcgg atgtgaccga cgctggattt caccacgtag tcactaccca gatacttgtt
     2581 gatcgttttg gcttttgccg gggactcaac gatgacaaga gctttaccca tattcacctt
     2641 acctaatttg attcttccag gaatgcgtcg cacgttgata aaccttccac tggcaacgag
     2701 ttaccgatat ggactcgttc tctgcggata tcaacccctt gtcgtgactc tgttcacgaa
     2761 aattcaggtg actgagttaa caggtcttgg cctacaggcg ctatagcacg acggaatttt
     2821 ggtcgaatgt caagcaaatc tattgccaga ttgacgaaag cgtcacactg tacctgataa
     2881 aatccgatac gcaactttat tagcatgcaa aatccaaagt tgccagtaag gcgctcttgc
     2941 caccagcgtg gtatatttgt ctgcaagcca tgcctggcgt agactaatgt ccttatttaa
     3001 ggagtaaata atgaacaaag agacacaacc catcgaccgc gaaacgctgt tgaaagaggc
     3061 caacaaaatc attcgtgagc atgaggatac gctcgcaggg attgaagcca ccggcgtcac
     3121 gcagcgtaat ggcgtgctgg tatttaccgg cgactacttt ttagatgaac aaggattgcc
     3181 aacagccaaa agcaccgcgg tgtttaatat gttcaaacac ctggcccatg tgctttcaga
     3241 aaaatatcat ctggttgatt aaagcaaaac gcgaggtctt agcctcgcgt ttgtctttta
     3301 catcaatggc ttttgacccc gctgccacca gcgtagcaac aatcgatcgg cgctctctgc
     3361 cgcgctgccg gtgaatcggt caatgagtcg tttacgctgc atatagcgta cattaaccac
     3421 ttcacggcct tccatcaggc taagaataac ttcatcactg gtgttgattt catcaaccag
     3481 gcctttctct accgcctgtt gtccgtacca gtgttcacct gttgccacct gttcaatatc
     3541 cagagacgga cgcatacgct tcacaaaatc tttaaacaac tgatgcgttt cgttcaactc
     3601 ttcgcggaat ttctcccgcc cttcttcggt attttcaccc agcaacgtca gagtacgctt
     3661 atactgcccg gcagtgtgca gttcgatatc aatatatttg cttttcagga agcggttaaa
     3721 gttgggcatt tgcgccacca ccccaatgga acccacaata gcaaacggtg cggaaacaat
     3781 tttgtccgcc acacaggcca tcatgtaacc gccgctggca gcgactttgt ctaccgtaac
     3841 agttaaagga atgtttttat cacgcagacg ctgcagctgc gaagccgcca acccgtaacc
     3901 atgcaccatg ccaccagggc tttccagacg gagcacaacc tgatcctgcg gtttgaatgc
     3961 tgcgagtacc gccgttatct cttcacgtag cgagttcact tcatgggcgt ccatgctgcc
     4021 tttaaaatcc agcacccaga cgcgaggttt actgtcagtt gccacctcgc ccagtttggc
     4081 tttcgctttt gctgctttcg cttcttgctt gtgctttttc ttctgcgctt tgtgccactg
     4141 tttttgctga tgtgagccca tcagcgccgc ggcaagttct tctttcatct ccttatactg
     4201 ttcgctgaga ttgttgaccc gtaactcgcc acgctggcgt ttattacgtt gagcaacatt
     4261 gacaataatg gcggcaatcg ccgcaatcgc tagcacaacg gtaacgattt tcgccaaaaa
     4321 caaaccatat tcagacaaca attccacgag tccccacctt ggttaaacaa cgctttggct
     4381 cacagtttac aacaggcatg ttaagccgtc tcgcagagat gttattgcag cgaaagtatg
     4441 acaaagtatc cattttgcgg tgttatcgcc ccaggctgat tgaaatgccc ttctgtttca
     4501 ggcataaagc cccaaagtca taaagtacac tggcagcgcg acagacaacg tcgttgagga
     4561 gtagccatgc attaccagcc aaaacaagat ttactcaatg atcgcattat cctggtgacg
     4621 ggagccagcg atgggattgg tcgtgaagcc gcgatgacgt atgcacgcta tggtgcgaca
     4681 gtgattctgt tgggccgtaa tgaagaaaaa ttacgtcagg tagccagcca cataaacgaa
     4741 gaaactgggc gtcagccaca gtggtttatt ctcgatttgc tgacctgcac gtccgaaaat
     4801 tgccaacaac tggcacagcg cattgtcgtt aattatccgc gtctggatgg tgttttgcat
     4861 aatgccggat tgctcggcga tatttgccca atgagcgaac aaaatccgca ggtctggcag
     4921 gacgtcatgc aggtcaacgt taatgccacc tttatgctca cccaggcact gcttccttta
     4981 ttactcaaat cggacgccgg ttcactggtc tttacttcat caagcgttgg acgtcaggga
     5041 cgagctaact ggggtgcata tgcagcgtcg aaatttgcca ccgaagggat gatgcaggta
     5101 ctggccgatg aatatcagca gcgcctgcgc gtcaactgca ttaacccagg cggtacgcgc
     5161 accgcaatgc gcgcaagcgc cttcccgacc gaagatccac agaaacttaa aacacccgct
     5221 gatatcatgc cgctctacct ctggctgatg ggcgatgaca gccgccgtaa aaccggcatg
     5281 acctttgacg cccaaccggg ccgtaaacca ggaatttccc aatgagtgat gaacgctacc
     5341 aacagcgtca gcagcgagtg aaagaaaaag tagatgctcg tgtggcacag gccccaggat
     5401 gaacgcggta ttatcatcgt ctttaccggc aatggaaaag gcaaaaccac cgcggcattt
     5461 ggtacggcaa cacgcgcagt tggtcacgga aaaaaagtgg gcgtcgtgca gtttattaaa
     5521 ggcacctggc ctaatggcga acgcaatctg ctggaaccac atggcgttga gtttcaggtg
     5581 atggcaacgg gctttacctg ggatacacaa aaccgcgagt ctgataccgc cgcctgccgc
     5641 gaagtctggc aacatgcaaa gcggatgctt gctgattcct cactggatat ggttttgctt
     5701 gatgaactga cgtatatggt ggcgtatgac tatttgccac tggaagaagt ggtgcaggcg
     5761 ttaaatgaac gtccacatca acagacggtg attatcacgg gtcgaggctg tcatcgggat
     5821 attcttgaac tggcggatac ggtaagtgag ctgcgcccca tcaaacatgc gtttgatgcc
     5881 ggtgttaagg cgcaaatagg gattgattat taatctgtaa aaatgccgga tatgacgtcc
     5941 ggcatacttt gattaaccgt tattacgccc gccagaacga cgaccgccgc tcacctgact
     6001 gtgacgtttc accgcacgac gaatctgatt agccttcatg cgacgacggt ctttttctac
     6061 agcgacttta gagctggttt ctggcggaag ctccaccagt tcgcgcagat agttagtctg
     6121 ggcgaggtcc agctccgtcc agccaccgcg tggcagacct tttggcagtg ggatatcacc
     6181 gtaacgaaca cggatcaggc ggctcacctg cacaccaacc gcttcccaca gacgacgaac
     6241 ttcacggtta cggccttcgg tcagagtcac gttgtaccac tggttgatcc cttcgccgcc
     6301 gctgaacttg atggttttaa aagctgccgg accatcttcc agttgcacgc cacgactcaa
     6361 atcacgcagt ttcgcgtcgt caacctgacc aaatacacgc acggcgtatt cacgttcaac
     6421 ttcacggctt ggatgcatta aacggtttgc cagttcacca tcggtggtga acagcagcag
     6481 accacaggtg ttaacgtcca gacgacctac ggcaatccag cgtgcgccac gcagctttgg
     6541 cagacggtca aacaccgtcg gacgcccttc cggatcgtta cgggtacaca actcaccttc
     6601 cggtttgtaa taggccagaa cgcgacaaat ttgttcagcg gactcacgta ccgaaatcag
     6661 atgaccatcg atacgaattt tcagcccagg ggtaacttca acgcgatcgc cgagtttagc
     6721 aattttgcca tcaacactca cacgaccggc ttcaataata gattcgattt cacggcggga
     6781 gccgtggcca gcgcgggcca gcactttctg taatttttcg ctcatagagc ttccttttgg
     6841 tgtcgccttc ccaggcgtct gatatgcttt tgtgcaacag tgagtcagaa cttacatctg
     6901 actcactgat agtaaagtct gtttgaactc ttcaggcctt aagtaaacga ctcgacgttt
     6961 atcggcctgg ctttgaggcg caacatacta cactatcttt atggttaaag cttgtttcga
     7021 ttaacatcag cttaattacc agccaccacc gcctccgcca cccgcgccgc cgccagcacc
     7081 accaccggaa taacttcgcc ccaacaaact ggtattggcc ccgcaactta ggtgcgtctg
     7141 taatgagaat aaaacgccgt tacgggcaat ctctggagcg cgaatcgccg aagaaagcga
     7201 aggggcaatg cgtaacgccc attccctccc caaatttgcc gatatagccc aacctagcaa
     7261 cgcgtaatcc agtttccgtg tttccccttt ccgccgacgt ccactgtgtg ttgctgcctc
     7321 tttacgcgca agatagttaa cgatagcttc accgcgggca taacgttgct gcccagtttg
     7381 ggtaaacttt ggcagaaaaa tataccccat gccagtgaga taaccggtca ggcaaacagc
     7441 gctaaaataa cccgctggca tataccagta aaagaataca tctccgacat tgatatagag
     7501 agaatagaag gccacgcctg aacaaatcag tggtaaaaac aaagtgatga ttgcacgatt
     7561 aacgcaaggc ttgtcatcat cgcgggcaaa caaaagctca agggaacaaa ataaaaaagg
     7621 aataatccag cagatcataa taaacatatc gcctaccaga ctcattcctg cccaacctac
     7681 gggactcagg ttaccgtaga gaatgatcgc caatattgcc agtccacccc attgcaaaaa
     7741 cgtatcaggc cggtaaaaca aagacttttt acgcggcaga taaaatttct gcatccgtaa
     7801 aaatgccctg cgtaagactc tgttgcgtct ccccgtaaga acgaccttat cgcctttacg
     7861 aaataacgct tccagtaata actgctctgc gggcagtaat acctcattat ttctggtcgc
     7921 gccttcatta acacaaatca ggatttgctg gttcttatca tactgatctt ccagatgaat
     7981 ttttcccttc acgatcagat ttacgatatc ggcacagaaa cctttatcgt cgtaaaccag
     8041 cttcgcatcg agacgtaaca tgccgggcgt gtaatctggc ggaatgacat cggtttcaat
     8101 cacatctacc ggcgtaaatt gcggcctgcg cttccagaga tataaccatc cacaaaccag
     8161 gaatagacac ggaaaccaaa ttaacaacga tgacgtggag ggtaaaagaa gatggctgaa
     8221 aatattcgtc gtttgtggag ccggggcatt gctaagtaac gcgtgaggcc agcggtagag
     8281 taccgtgaaa tcttcacgat aaaacggatc gcgggattca attcttccgt cctcaaggat
     8341 gcggccattt cgatatgtgt cgccctcttc tccagtaaag agatcgattt cgctaaatgg
     8401 attacccgcg atatcaggga acttgagtga aaatcgggtc ttatagattt caaacggcca
     8461 gtggttacca gtcacgttcc agattaacag atctgaatct ccctcacgca ggaaagcatt
     8521 tttaacctga taatgaattt gataatgata gtcgccttga ggcaagaaac gttgtttatc
     8581 gcccacgaca atactcatta acccgatatg ccagtcaaga tgccagggtt caggaatacc
     8641 atcacgagtg acgccaacaa tatgatagtt cagcagtgca gcgtccccat caggctgcat
     8701 ccagagtcgt ggtaaatcac gataaaatcc gtgcttaatc tgctgatttc gcgcctgaaa
     8761 atgaatattt tctttgatgt cgagcacccc atccggttgc agagtggctt caacatcaaa
     8821 gagcgagatc ccttcttcat aagaagggat ctccgggttt tctgcataac ttagagatga
     8881 tacaaacagc cctgctatca acagcaaaat gcagcgaaac ttccctgcca tagtcttcca
     8941 tccgtggaac gcaatttgtt ataagaaagg cttcacatca cccacgcctt cacgcaccac
     9001 gaccggcgta tcatcggtaa gatcaatgac cgttgtcggt ttctgtccga gatagccgcc
     9061 gtggataatc aaatccacct gtttttccag acggtcttta atttcttctg gatcggattc
     9121 ggtaaattcg ctgcctggca gcattagcga agtggaaagc atcggttcgc ccagtgcttc
     9181 aagcaacgcc tgcgcaatcg ggttagaagg cacacgcata ccgatggttt tgcgtttttc
     9241 ctgcaacaga cggcgtggca cctccttcgt ccccttcagg atgaaggtat agttgcccgg
     9301 cgtgttgttt ttcattaaac gaaacgcaac gttatccacg aatgaatagg tcgaaagttc
     9361 agaaagatcg cgacacatca gggtaaagtt gtgaccgtcc ggcagctggc gaatacgaca
     9421 aatacgctcc atcgcgtttt tatcttcaat tttacagccg agcgcatagc cggaatcagt
     9481 tggataaaca atcaccccgc ctttacgcac gatctccacc gcctggttga tcagacgttg
     9541 ctgcgggtta tcaggatgaa tataaaaaaa ctggctcata attccctctc tgtggtgttc
     9601 tgcggctgtt cccatagctg ccagacgcct tcaacgcctg cgggcagcca gagtttacga
     9661 cccagttcga tccacgggca tggctgatga aaatcagatc cttgtgatgc ccataaatga
     9721 tgctgacgcg caagtgtcgt caactgggtg cgttcattgg gcgattgctg gcactgcgcg
     9781 acttccatcg cgtcgccgtg gtgttcggca aaatgcgcaa ccaatctttt cagccattta
     9841 gcactaagat tgtaccgccc aggatgagct agcaccgcct taccgccaga atgatgaatg
     9901 acatcaatag cttgttctat tgtacaccac tgtggcggaa cgtatccggt tttcccgcgc
     9961 gccagatact ttttaaagac atctgccatt gaactggctt tgccgcactc aacaaggaaa
    10021 cgcgcaaaat gaccgcgcgt cactgcgccg ccctgcgcca gtcgttgtgc gccttccagc
    10081 gccccaggta tttgcgcttt ttcaagtctt tcggcaattt gctgcgcccg ctgattgcgc
    10141 cgttctgtct gctgcgcgag gaactcacac attagcggat gagtaatatc aatattcagc
    10201 ccgacaatat gaatttcatg attttcccag accgtggaaa tttccacgcc gggaataaga
    10261 ttcagcgcca gtcccgaacg tgaaatttcc tctcttgctg gcgcgattgc agctgtggtg
    10321 tcatggtcgg tgatcgccag ggtgccgacg cgcatctcga ctgcacggtg caccaatgct
    10381 tctggcgtca ggcagccatc ggaagctgtg gtatggctgt gcaggtcgta aatcactgcg
    10441 taattcgtgt cgctcaaggc gcactcccgt tctggataat gttttttgcg ccgacatcat
    10501 aacggttctg gcaaatattc tgaaatcagc tgttgacaat taatcatcga actagttaac
    10561 tagtacgcaa gttcacgtaa aaagggtatc gacaatgaaa gcaattttcg tactgaaagc
    10621 ttggtggcgc acttcctgaa acgggcagtg tattcaccat gcgtaaagca atcagatacc
    10681 cagcccgcct aatgagcggg cttttttttg aacaaaatta gagaataaca atgcaaacac
    10741 aaaaaccgac tctcgaactg ctaacctgcg aaggcgctta tcgcgacaac ccgactgcgc
    10801 tttttcacca gttgtgtggg gatcgtccgg caacgctgct gctggaatcc gcagatatcg
    10861 acagcaaaga tgatttaaaa agcctgctac tggtagacag tgcgctgcgc attacagcat
    10921 taggtgacac tgtcacaatt caggcacttt ccggcaacgg cgaagccctg ctgacactac
    10981 tggataacgc cctgcctgcg ggtgtggaaa atgaacaatc accaaactgc cgtgtgctgc
    11041 gcttcccccc tgtcagtcca ctgctggatg aagacgctcg cttatgctcc ctttcggttt
    11101 ttgacgcttt ccgtttattg cagaatctgt tgaatgtacc gaaggaagaa cgagaagcaa
    11161 tgttcttcgg cggcctgttc tcttatgacc ttgtggcggg atttgaagat ttaccgcaac
    11221 tgtcagcgga aaataactgc cctgatttct gtttttatct cgctgaaacg ctgatggtga
    11281 ttgaccatca gaaaaaaaac acccgcattc aggccagcct gtttgctccg aatgaagaag
    11341 aaaaacaacg tctcactgct cgcctgaacg atcttcgcca gcagctgacc gaagccgcgc
    11401 cgccgctgcc ggtggtttcc gtgccgcata tgcgttgtga atgtaaccag agcgatgaag
    11461 agttcggtgg cgtggtgcgt ttgttgcaaa aagcgattcg caccggagaa attttccagg
    11521 tggtgccgtc tcgccgtttc tctctgccct gcccgtcacc gctggcggcc tattacgtgc
    11581 tgaaaaagag taatcccagc ccgtacatgt tttttatgca ggataatgat ttcaccctgt
    11641 ttggcgcgtc gccggaaagt tcgctcaagt atgacgccac cagccgccag attgagatct
    11701 acccgattgc cggaacacgc ccgcgcggtc gtcgcgccga tggttcactg gacagagacc
    11761 tcgacagccg catcgaactg gaaatgcgta ccgatcataa agagctttct gaacatctga
    11821 tgctggtgga tctcgcccgt aatgatctgg cacgcatttg cacccccggc agccgctacg
    11881 tcgccgatct taccaaagtt gaccgttact cttacgtgat gcacctggtc tcccgcgtgg
    11941 tcggtgagct gcgccacgat ctcgacgccc tgcacgctta ccgcgcctgt atgaatatgg
    12001 ggacgttaag cggtgcgccg aaagtacgcg ccatgcagtt aattgccgag gcggaaggtc
    12061 gtcgccgcgg cagctacggc ggcgcggtag gttatttcac cgcgcatggc gatctcgaca
    12121 cctgcattgt gatccgctcg gcgctggtgg aaaacggtat cgccaccgtg caagccggtg
    12181 ctggcatagt ccttgattct gttccgcagt cggaagccga cgaaacccgt aataaagccc
    12241 gcgctgtact gcgcgctatt gccaccgcgc atcatgcaca ggagactttc tgatggctga
    12301 cattctgctg ctcgataata tcgactcttt tacgtacaac ctggcagatc agttgcgcag
    12361 caatgggcat aatgtggtga tttaccgcaa ccatattccg gcgcagacct taattgaacg
    12421 cctggcgacg atgagcaatc cggtgctgat gctttctcct ggccccggtg tgccgagcga
    12481 agccggttgt atgccggaac tcctcacccg cttgcgtggc aagctgccca ttattggcat
    12541 ttgcctcgga catcaggcga ttgtcgaagc ttacgggggc tatgtcggtc aggcgggcga
    12601 aattctccac ggtaaagcct ccagcattga acacgacggt caggcgatgt ttgccggatt
    12661 aacaaacccg ctgccagtgg cgcgttatca ctcgctggtt ggcagtaaca ttccggccgg
    12721 tttaaccatc aacgcccatt ttaatggcat ggtgatggca gtacgtcacg atgcggatcg
    12781 cgtttgtgga ttccagttcc atccggaatc cattcttact acccagggcg ctcgcctgct
    12841 ggaacaaacg ctggcctggg cgcagcggaa actagagcca accaacacgc tgcaaccgat
    12901 tctggaaaaa ctgtatcagg cgcagacgct tagccagcaa gaaagccacc agctgttttc
    12961 agcagtggta cgtggcgaac tgaaaccgga acaactggcg gcggcgctgg tgagcatgaa
    13021 aattcgcggc gagcacccga acgagatcgc cggagcagca accgcgttac tggaaaacgc
    13081 cgcgccgttc ccgcgcccgg attatctgtt tgccgatatc gtcggcaccg gtggtgacgg
    13141 cagtaacagc atcaatattt ccaccgccag tgcgtttgtc gccgcggcct gtgggctgaa
    13201 agtggcgaaa cacggcaacc gtagcgtctc cagtaaatct ggttcgtccg atctgctggc
    13261 ggcgttcggt attaatcttg atatgaatgc cgataaatcg cgccaagcgc tggatgagtt
    13321 aggcgtatgt ttcctctttg cgccgaaata tcacaccgga tttcgccatg cgatgccggt
    13381 tcgccagcaa ctgaaaaccc gcactctgtt caacgtgctg ggaccattga ttaacccggc
    13441 gcatccgccg ctggcgttaa ttggtgttta tagtccggaa ctggtgctgc cgattgccga
    13501 aaccttgcgc gtgctggggt atcaacgcgc ggcggtagtg cacagcggcg gtatggatga
    13561 agtttcatta cacgcgccga caatcgttgc cgaactgcat gacggtgaaa ttaagagcta
    13621 tcagctaact gcagaagatt ttggcctgac accctaccac caggagcaac tggcaggcgg
    13681 aacaccggaa gaaaaccgtg acattttaac acgcttgtta caaggtaaag gcgacgccgc
    13741 tcacgaagca gccgtcgcgg cgaatgtcgc catgttaatg cgcctgcatg gccatgaaga
    13801 tctgcaagcc aatgcgcaaa ccgttcttga ggtactgcgc agtggttccg cttacgacag
    13861 agttaccgca ctggcggcac gagggtaaat gatgcaaacc gttttagcga aaatcgtcgc
    13921 agacaaggcg atttgggtag aaacccgcaa acagcagcaa ccgctggcca gttttcagaa
    13981 tgaggttcag ccgagcacgc gacattttta tgatgcgcta cagggtgcac gcacggcgtt
    14041 tattctggag tgcaagaaag cgtcgccgtc aaaaggcgtg atccgtgatg atttcgatcc
    14101 ggcacgcatt gccgccattt ataaacatta cgcttcggca atttcggtgc tgactgatga
    14161 gaaatatttt caggggagct ttgatttcct ccccatcgtc agccaaatcg ccccgcagcc
    14221 gattttatgt aaagacttca ttatcgaccc ttaccagatc tatctggcgc gctattacca
    14281 ggccgatgcc tgcttattaa tgctttcagt actggatgac gaccaatatc gccagcttgc
    14341 agccgtcgct cacagtctgg agatgggggg gctgaccgaa gtcagtaatg aagaggaact
    14401 ggagcgcgcc attgcattag gggcaaaggt cgttggcatc aacaaccgcg atctgcgtga
    14461 tttgtcgatt gatctcaacc gtacccgcga gcttgcgccg aaactggggc acaacgtaac
    14521 ggtaatcagc gaatccggca tcaatactta cgctcaggtg cgcgagttaa gccacttcgc
    14581 taacggtttt ctgattggtt cggcgttgat ggcccatgac gatttgcacg ccgccgtgcg
    14641 ccgggtgttg ctgggtgaga ataaagtatg tggcctgacg cgtgggcaag atgctaaagc
    14701 agcttatgac gcgggcgcga tttacggtgg gttgattttt gtcgcgacat caccgcgttg
    14761 cgtcaacgtt gaacaggcgc aggaagtgat ggctgcggca ccgttgcagt atgttggcgt
    14821 gttccgcaat cacgatattg ccgatgtggt ggacaaagct aaggtgttat cgctggcggc
    14881 agtgcaactg catggtaatg aagatcagct gtatatcgat acgctgcgtg aagctctgcc
    14941 aacacatgtt gccatctgga aagcattaag cgtcggtgaa accctgcccg cccgcgagtt
    15001 tcagcacgtt gataaatatg ttttagacaa cggccagggt ggaagcgggc aacgttttga
    15061 ctggtcacta ttaaatggtc aatcgcttgg caacgttctg ctggcggggg gcttaggcgc
    15121 agataactgc gtggaagcgg cacaaaccgg ctgcgccgga cttgatttta attctgctgt
    15181 agagtcgcaa ccgggcatca aagacgcacg tcttttggcc tcggttttcc agacgctgcg
    15241 cgcatattaa ggaaaggaac aatgacaaca ttacttaacc cctattttgg tgagtttggc
    15301 ggcatgtacg tgccacaaat cctgatgcct gctctgcgcc agctggaaga agcttttgtc
    15361 agcgcgcaaa aagatcctga atttcaggct cagttcaacg acctgctgaa aaactatgcc
    15421 gggcgtccaa ccgcgctgac caaatgccag aacattacgg ctgggacgaa caccacgctg
    15481 tatctgaagc gcgaagattt gctgcacggc ggcgcgcata aaactaacca ggtgctcggt
    15541 caggctttac tggcgaagcg gatgggtaaa accgaaatca tcgccgaaac cggtgccggt
    15601 cagcatggcg tggcgtcggc ccttgccagc gccctgctcg gcctgaaatg ccgtatttat
    15661 atgggtgcca aagacgttga acgccagtcg cctaacgttt ttcgtatgcg cttaatgggt
    15721 gcggaagtga tcccggtaca tagcggttcc gcgacgctga aagatgcctg taacgaggcg
    15781 ctgcgcgact ggtccggcag ttatgaaacc gcgcactata tgctgggcac cgcagctggc
    15841 ccacatcctt atccgaccat tgtgtgtgag tttcagcgga tgattggcga agaaacgaaa
    15901 gcgcagattc tggaaagaga aggtcgcctg ccggatgccg ttatcgcctg tgttggcggc
    15961 ggttcgaatg ccatcggcat gtttgcagat ttcatcaacg aaaccaacgt cggcctgatt
    16021 ggtgtggagc cgggtggtca cggtatcgaa actggcgagc acggcgcacc gctaaaacat
    16081 ggtcgcgtgg gcatttattt cggtatgaaa gcgccgatga tgcaaaccga agacgggcag
    16141 attgaagaat cttactccat ctccgccgga ctggatttcc cgtctgtcgg cccacaacac
    16201 gcgtatctta acagcactgg acgcgctgat tacgtgtcta ttaccgatga tgaagccctt
    16261 gaagccttca aaacgctgtg cctgcacgaa gggattatcc cggcgctgga gtcttcccac
    16321 gccctggccc atgcgctgaa aatgatgcgc gaaaccccgg aaaaagagca gctactggtg
    16381 gttaaccttt ccggtcgcgg cgataaagac atcttcaccg ttcacgatat tttgaaagca
    16441 cgaggggaaa tctgatggaa cgctacgaat ctctgtttgc ccagttgaag gagcgcaaag
    16501 aaggcgcatt cgttcctttc gtcacgctca gggatccggg cattgagcag tcattgaaaa
    16561 ttatcgatac gctaattgaa gccggtgctg acgcgctgga gttaggtatc cccttctccg
    16621 acccactggc ggatggcccg accattcaaa acgccactct gcgcgccttt gcggcaggtg
    16681 tgactccggc acaatgtttt gaaatgctgg cactgattcg ccagaaacac ccgaccattc
    16741 ccattggcct gttgatgtat gccaatctgg tgtttaacaa aggcattgat gagttttatg
    16801 cccagtgcga gaaagtcggc gtcgattcgg tgctggttgc cgatgtgcca gttgaagagt
    16861 ccgcgccctt ccgccaggcc gcgttgcgcc ataatgtcgc acctatcttc atctgcccgc
    16921 caaatgccga tgacgacctg ctgcgccaga tagcctctta cggtcgtggt tacacctatt
    16981 tgctgtcacg agcgggcgtg accggcgcag aaaaccgcgc cgcgttaccc ctcaatcatc
    17041 tggttgcgaa gctgaaagag tacaacgctg cacctccatt gcaggggttt ggtatttccg
    17101 ccccggatca ggtaaaagca gcgattgatg caggagctgc gggcgcgatt tctggttcgg
    17161 ccattgttaa aatcatcgag caacatatta atgagccaga gaaaatgctg gcggcactga
    17221 aagtttttgt acaaccgatg aaagcggcga cgcgcagtta atcccacagc cgccagttcc
    17281 gttggcggca ttttaacttt ctttaatgaa gccggaaaaa tcctaaattc atttaatatt
    17341 tatcttttta ccgcttcgct tacccctgtc gaacgtcaac ttacgtcatt tttccgccca
    17401 acagtaatat aatcaaacaa attaatcccg caacataaca ccagtaaaat caataatttt
    17461 ctctaagtca cttattcctc aga
//
LOCUS       DADLFA010000081        17459 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000081 DADLFA010000000
VERSION     DADLFA010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17459
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.081"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            224..667
                     /locus_tag="JQH65_002267"
     CDS             224..667
                     /locus_tag="JQH65_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY6139664.1"
                     /translation="MDLALVTGQREGDLAAMRWEDIRDGRLYVEQQKTGAKIRISLPT
                     TISRLNLTLTDVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            903..1721
                     /locus_tag="JQH65_002268"
     CDS             903..1721
                     /locus_tag="JQH65_002268"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
                     /protein_id="HAY6139665.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLRPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDMPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1722..2780)
                     /gene="modC"
                     /locus_tag="JQH65_002269"
     CDS             complement(1722..2780)
                     /gene="modC"
                     /locus_tag="JQH65_002269"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HAY6139666.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2783..3472)
                     /gene="modB"
                     /locus_tag="JQH65_002270"
     CDS             complement(2783..3472)
                     /gene="modB"
                     /locus_tag="JQH65_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HAY6139667.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3472..4245)
                     /gene="modA"
                     /locus_tag="JQH65_002271"
     CDS             complement(3472..4245)
                     /gene="modA"
                     /locus_tag="JQH65_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY6139668.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFIIDSTTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVVSKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4411..4560)
                     /locus_tag="JQH65_002272"
     CDS             complement(4411..4560)
                     /locus_tag="JQH65_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump-associated protein, AcrZ
                     family"
                     /protein_id="HAY6139669.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4689..5477
                     /gene="modE"
                     /locus_tag="JQH65_002273"
     CDS             4689..5477
                     /gene="modE"
                     /locus_tag="JQH65_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HAY6139670.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNANNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5545..7017
                     /gene="modF"
                     /locus_tag="JQH65_002274"
     CDS             5545..7017
                     /gene="modF"
                     /locus_tag="JQH65_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HAY6139671.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKILLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQHLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7278..8294
                     /gene="galE"
                     /locus_tag="JQH65_002275"
     CDS             7278..8294
                     /gene="galE"
                     /locus_tag="JQH65_002275"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAY6139672.1"
                     /translation="MRVLLTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGIGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8304..9350
                     /gene="galT"
                     /locus_tag="JQH65_002276"
     CDS             8304..9350
                     /gene="galT"
                     /locus_tag="JQH65_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HAY6139673.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNHDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9354..10502
                     /gene="galK"
                     /locus_tag="JQH65_002277"
     CDS             9354..10502
                     /gene="galK"
                     /locus_tag="JQH65_002277"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAY6139674.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLAEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10496..11536
                     /gene="galM"
                     /locus_tag="JQH65_002278"
     CDS             10496..11536
                     /gene="galM"
                     /locus_tag="JQH65_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HAY6139675.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11738..12490
                     /gene="gpmA"
                     /locus_tag="JQH65_002279"
     CDS             11738..12490
                     /gene="gpmA"
                     /locus_tag="JQH65_002279"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAY6139676.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12648..13700)
                     /gene="aroG"
                     /locus_tag="JQH65_002280"
     CDS             complement(12648..13700)
                     /gene="aroG"
                     /locus_tag="JQH65_002280"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HAY6139677.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            14016..14396
                     /locus_tag="JQH65_002281"
     CDS             14016..14396
                     /locus_tag="JQH65_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6139678.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAATDAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14510..15451
                     /gene="zitB"
                     /locus_tag="JQH65_002282"
     CDS             14510..15451
                     /gene="zitB"
                     /locus_tag="JQH65_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HAY6139679.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSIVVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHAHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(15448..16167)
                     /locus_tag="JQH65_002283"
     CDS             complement(15448..16167)
                     /locus_tag="JQH65_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HAY6139680.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIH
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMLLMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSCALSH"
     gene            complement(16205..17248)
                     /gene="nadA"
                     /locus_tag="JQH65_002284"
     CDS             complement(16205..17248)
                     /gene="nadA"
                     /locus_tag="JQH65_002284"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HAY6139681.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
BASE COUNT         4433 a         4544 c         4362 g         4120 t
ORIGIN      
        1 tcggaaaatc gatactggaa aaactctaac tcggtggcca gtttttgttg accacttcac
       61 agacattttt aaaacagcta tagcttctgg tcttgccgaa gaagatccag cttctgcaac
      121 caggccattc agaactgaag tgaagcgcag tcgactgtta attgacgagt atctgttaat
      181 caggaaaata gcagacgtcc agaacgaatg gttcggcctt tgcatggatc ttgcactggt
      241 aacaggacag cgcgaaggag atctggcggc tatgagatgg gaggatataa gagatggcag
      301 actatacgtg gaacaacaaa aaacaggggc aaaaattcgc atttctctcc ccaccacgat
      361 ttccagattg aatttaacac ttaccgatgt actggataac ttgaaaaaaa taaacggtaa
      421 aaacgaaaag ttactcggag ggaagacagc caggacaatc gcagcacagt tcaggattgc
      481 ccgagacaca tccggattga aatgggaagg tgacccaccg ccatttcatg aaatccgaag
      541 tttatccggt cgcctgcact cagcagaaaa aggcagtgat ttcactcagg cgctccttgg
      601 acatcgttca tccagcatga cggataaata ccgtgacgga cgagggaggg aatggaaaga
      661 catttagaat taatttcgaa tgatttcgaa acaccacttg taattaattg attataaagg
      721 attaattcct aagaggcatt tgcttcatga atgctccttt gcatttagca actgactgta
      781 acagctaaaa ttagtcgctt ttggcggtga atgctgaatt aaaacgcaga attttgtccg
      841 atagtggtat cggtgtacca ttcgcgcaga cgaaaattct taaccttaat ctggaatacg
      901 ccatgaccac acgcgtgatt gctctcgact tagacggcac cttattgacc ccgaaaaaga
      961 ccctgcgtcc ttcatcgata gaagccctgg cccgcgctcg cgaagcaggc tatcaattaa
     1021 tcatcgtcac aggtcgccat cacgtcgcta ttcatccttt ttatcaggcg ctggcgctgg
     1081 atacacctgc tatttgctgt aatggcacct atttgtatga ttatcatgca aaaaccgtgc
     1141 tggaagcgga cccaatgccc gttaataaag ccctgcaact cattgagatg ctgaatgaac
     1201 accacattca cggtctgatg tatgtggatg atgcaatggt ctatgagcac ccgaccgggc
     1261 atgtcattcg cacgtctaac tgggcgcaaa ccctgccgcc ggaacagcgt ccgactttca
     1321 cacaagtcgc ttctctggct gaaacggcgc aacaagttaa cgccgtatgg aagttcgccc
     1381 tgacgcacga tgacatgccg caattgcagc attttggtaa gcatgtcgaa catgaactgg
     1441 gactggagtg tgaatggtcc tggcacgatc aggttgatat tgcacgcggc ggcaacagca
     1501 aaggtaaacg tttgacgaaa tgggttgagg cgcaaggctg gtcgatggaa aacgtcgtgg
     1561 cgttcggcga taactttaat gatatcagta tgctggaggc cgctggtaca ggcgtggcga
     1621 tgggcaacgc cgatgacgcg gtaaaagcgc gcgccaacat tgtgattggt gataacacca
     1681 ccgacagcat tgcccagttc atttatagcc acctgattta atcaggcggt tatcgacaca
     1741 cttttaattt gcgcgtacag ccacaggcct ggtttgatcg ccagttcatc cctggcccac
     1801 gggctgatac gcgcccacag cgttttaccg ccgacttcca gctccacttc cacctgaccg
     1861 ttgtcgtcat aactattaac aacttttgcc cgcaatacgt tacgaatgct ggtttgctgc
     1921 ggcggttgta aaaccagaga aacatcggaa gcctgaatgc ggatacgtag cgcagcttgc
     1981 agcggttcgt ccagcttatt gacccacaaa tgctgatcgc ccagcgccag cgcggtcatc
     2041 gcgtaatgcg gatgatgctc aagcaccgtc actttcagaa tgctactttg ttgctctttc
     2101 ggcagccacg gattcatcac gctactaccc cagacttctt ccagtgcgcc aaaggctttc
     2161 acctgaccgt tttccagtac catcactctg tctgccagat ggaggatctc atccagcgaa
     2221 tggctgacat acaacatcgg gatgttgatt tcccgcgtca gacgttgcag ataaggcaac
     2281 agttcgcgtt tacgcggaat atccagtgac gccagcggtt catccagcaa cagcaattct
     2341 ggtgccgtca gcaaagcccg cccaatcgct acgcgctgtt tttcgcctcc ggacaggctg
     2401 cctggtaaac ggtcaagcaa cggttcaatg cctaaaagcg ccaccagctt atcgaactga
     2461 tcgaccatgc ttttcgccat gccgtagcgc agattgccac gcactttgta atgcggaaac
     2521 agccgcgcat cctgaaaaac atagccaacg cgacgctttt ctggcgtcag gcagatacct
     2581 ttttcggcat catttagtac ccgaccattg aggacaatcc tccctttttg cgggcgcgtc
     2641 agtccactga tggcgttaat cagcgaagtt tttccggcac cggagacgcc aaagatagca
     2701 gtgatgccat tggcgggcag cgtttcatta atagtcaggc aatggttgcc caacgtctgg
     2761 gaaaaattca gttccagcat gattagcgcc ccgcccgttc acggctgatt ctggccagcc
     2821 attctgaaat caacagggag atcatcgcca gcgcaataga aataatgcac agtctcgccg
     2881 cgccactttc cccgccgggg gtctggatca gggtatacat ggcagaggga atggttcgcg
     2941 tttcgccagg aatgttcgac acaaaggtga tggttgcacc aaactcaccg agagaacgag
     3001 caaaagccag taccgtacca acaataattc ccggtaaggt cagcggtaac gtgatagtaa
     3061 agaaaacgcg ccagcgcccg gcacctagtg ttcttgcggc ctgttcgagt ttgacatcaa
     3121 ccccttccag cgccagacga attgcccgca ccatcagcgg aaacgacatg acggcggcag
     3181 cgagaaccgc gccgcgccag ctaaaggcga aggtaatacc aaaccagtca tacagacgtt
     3241 caccgataaa tccgcgccgt cccatcgaaa ctaataataa gtaaccgacg accacgggcg
     3301 gtaacaccag cggtagatgc agtacgctgt cgagcagagc tttgcccgga aacgtgcaac
     3361 gcaccagtaa ccaggcaaaa aagatcccaa acggcaggct aaacagcaca gccagggaag
     3421 aaactttcag gcttaataaa actgcctgcc attctggatc ggtcagtatc attacttggt
     3481 tgtaaatccg taacgtttaa agatttccgc tgcctgcggt cccttcagat aatcataaaa
     3541 tgctttcact gtcgcattgt tatgcccttc cacaacagca accggatatt ccactttttt
     3601 atgtgaatct tccgggaagg tggcaaccac ttttacccct ttgctgacaa ctgcgtcaga
     3661 accgtaaaca atgcccaacg gcgcttcatt gcgttcgacc aacgccagcg cgccacgaac
     3721 atcttccgcc ggagccaatt tcggggagag cgtatcccat gcgcccagtt tttgcagtgc
     3781 ttcttttgca taaatgccag cagggacatg ttccggatcg ccaaccgcga ggcgaccgcc
     3841 attcagcagc gaagtccagt tagttgtgct gtcgatgatg aaatctttct gctcgctggc
     3901 tttcggtgct acaacgacca ggctattgcc gagcagtgtc tggcgcgaag ccgtatcgat
     3961 cgctttttta tcaaccgcat aatccatcca tttctgatcg gcagagataa acagatccgc
     4021 aggcgctccc gcttcaatct gacgggccag agtagatgac gaagcgaaag aagaaaccac
     4081 atccacgcct ttttcttttt tatactgcgt agcaatgtcc tgcattgcgt tagtcagtga
     4141 tgcggcggcg aacaccgtga ttttcccttc atctgccagt gtattgccag caacagcgaa
     4201 agagagtgcc gccccggcaa acaagttcaa ccatttacga gccattggta accccttaat
     4261 gtaacgttat ataggtgaca atataacggc taactcagaa ttttcccagc agttattgct
     4321 aacctttgtg aggtagataa gaaaaaatat cggctggaaa agcaggaagt tgagaaaaag
     4381 aaatgcccga ctaagtgggc attcagggaa tcaatgattt tgtccgggct ggtctttttt
     4441 accaacacca gaaaagatgt tgaatacttc accaagaccg taaatcagac ccaggatgat
     4501 ggccatcacg acaggtacca tgattacggc gaataccaga ctttttaata actctaacat
     4561 ggtcaactcc agatatagtc atgagactat tctaaccgct aagcacagaa aagcactccc
     4621 cttttgtgcg gtcagctttg cgtggcgttc gttttccgtc acaataagac ttttgccagg
     4681 acattgttat gcaggccgaa atccttctca cccttaagct ccaacaaaaa ttattcgccg
     4741 acccgcgccg gatttcgcta ctaaaacaca ttgcgctttc cggttccatt agccagggag
     4801 cgaaagatgc cggtatcagc tataaaagcg cctgggatgc cattaacgag atgaatcagt
     4861 taagcgagca tattctggtt gagcgcgcaa caggcggtaa aggtggcggc ggcgcagtac
     4921 tgacccgcta tggtcagcga ctgattcagc tctatgactt actggcgcaa atccagcaaa
     4981 aagcctttga tgtgttaagt gacgatgacg ccctgccgct gaacagcctg ctggccgcga
     5041 tctcacgttt ttcactgcaa accagcgccc gtaaccagtg gttcggtacc atcaccgccc
     5101 gcgatcatga tgacgttcaa cagcatgttg atgtcttact ggctgacgga aaaacacgcc
     5161 tgaaagtcgc aattaccgca caaagcggcg cacgtctggg gctggatgaa ggcaaagaag
     5221 tgttgatatt gctaaaagcg ccgtgggtag gcattactca ggacgaagcg gtcgcacaaa
     5281 acgccaacaa ccaattaccg ggtattatta gtcatattga gcgcggcgca gagcagtgcg
     5341 aagtattaat ggcgctaccc gacgggcaaa cactgtgcgc cacagtgccg gtaaatgaag
     5401 cgacttctct tcagcaaggg cagaatgtca cggcctactt taatgccgac agcgtgatta
     5461 tcgccacgct gtgctaagcg tgttgacaat ttgttatgaa acacgtatcc ctgtcagtaa
     5521 tcgctgcaca aaatgggata taaaatgtca tcgttgcaaa ttttgcaagg cacgtttcgt
     5581 cttagcgaca caaaaacgct gcaattgcct cagctaacgt taaacgcggg tgatagttgg
     5641 gcgtttgtcg gttcgaatgg aagcgggaaa tcggccctgg cccgcgcgct ggcgggggaa
     5701 cttccgcttt tgaaaggtga acggcaaagc cagttttccc acatcactcg tctctccttc
     5761 gagcaattgc aaaaactcgt cagcgacgaa tggcagcgga ataacaccga tatgctcggc
     5821 cctggcgaag atgacaccgg acgcactacg gctgagatca ttcaggatga agtaaaggat
     5881 gcaccgcgtt gcatgcaact ggcgcagcag ttcggtatta ccgccctcct cgaccgacgc
     5941 tttaaatacc tttccactgg cgagacgcga aaaatcctgc tgtgtcaggc gctgatgtcg
     6001 gagcctgact tgttgattct tgatgagccg ttcgatggcc tggatgttgc ctcacgtcag
     6061 cagctggctg agcgactcgc ctcgttacat cagtccggta ttactctggt actggtgctc
     6121 aatcgcttcg atgagatccc ggagtttgtc cagtttgctg gcgtgctggc ggattgcacg
     6181 ttagcggaaa ctggcgctaa agaggaactg ctccaacaag cactcgtcgc gcaactggca
     6241 catagtgaac agcttgaagg tgtgcaactg ccggagccgg atgaaccttc agcacgtcac
     6301 gccttacccg ccaacgaacc gcgcattgtg ctgaacaatg gcgtggtttc ttataacgat
     6361 cgccccattc ttaataacct tagctggcag gtgaatccag gtgaacactg gcaaattgtc
     6421 gggccaaatg gtgcaggaaa atcgacgtta ttaagcctgg ttactggcga tcatccgcaa
     6481 ggttacagca acgatttgac gcttttcgga cgacgtcgcg gcagcggcga aaccatctgg
     6541 gatatcaaaa agcatatcgg ttacgtcagc agtagtttgc atctggatta ccgggtcagc
     6601 actaccgtgc gtaatgtgat tctttctggc tattttgatt cgattggcat ttatcaggcc
     6661 gtttcggatc gccagcaaaa actggtgcag cagtggctgg atattctcgg cattgataaa
     6721 cgtacggctg acgctccgtt ccatagtctt tcctggggac agcagcatct ggcgctgatc
     6781 gtccgcgcac tggtgaaaca tccgacgttg cttattctcg atgaaccact acaggggctt
     6841 gatccgctca atcgccagct tatccgccgt tttgttgatg tgctgattag cgaaggtgaa
     6901 acgcaattgt tgtttgtttc gcaccacgct gaagatgcgc ctgcctgtat tacccatcgc
     6961 cttgagttcg tgccggacgg tggactctat cgctatgtgc tgacaaaaat atattgagtc
     7021 ggtagtgctg accttgccgg aggcggccta agcgccctct ccggccaacg gttcgacgca
     7081 tgcaggcatg aaaccgcttc ttttttcaga taaaaagcgc aatcattcat aaaccctctg
     7141 ttttataatc acttaatcgc gcataaaaaa cggctaaatt cttgtgtaaa cgattccact
     7201 aatttattcc atgtcacact tttcgcatct ttgttatgct atggttattt cataccataa
     7261 gcctaatgga gcgaattatg agagttctgc ttaccggtgg tagcggttac attggaagtc
     7321 atacctgcgt gcaattactg caaaacggtc atgatgtcat cattcttgat aacctctgta
     7381 acagtaagcg cagcgtactg cctgttatcg agcgtttagg cggcaaacat ccaacgtttg
     7441 ttgaaggcga tattcgtaac gaagcgttga tgaccgagat cctgcacgat cacgctatcg
     7501 acaccgtgat ccacttcgcc gggctgaaag ccgttggcga atcggtacaa aaaccgctgg
     7561 aatattacga caacaatgtc aacggtactc tgcgcctgat tagcgccatg cgcgccgcta
     7621 acgtcaaaaa ctttattttt agctcctccg ccaccgttta tggcgatcag cccaaaattc
     7681 catacgttga aagcttcccg accggcacac cgcaaagccc ttacggcaaa agcaagctga
     7741 tggtggaaca gatcctcacc gatctgcaaa aagcccagcc ggactggagc attgccctgc
     7801 tgcgctactt caacccggtt ggcgcgcatc cgtcgggcga tatgggcgaa gatccgcaag
     7861 gcattccgaa taacctgatg ccatacatcg cccaggttgc tgtaggccgt cgcgactcgc
     7921 tggcgatttt tggtaacgat tatccgaccg aagatggtac tggcgtacgc gattacatcc
     7981 acgtaatgga tctggcggac ggtcacgtcg tggcgatgga aaaactggcg aacaagccag
     8041 gcgtacacat ctacaacctc ggcgctggca taggcagcag cgtgctggac gtggttaatg
     8101 ccttcagcaa agcctgcggc aaaccggtta actatcattt tgcaccgcgt cgcgagggcg
     8161 accttccggc ctactgggcg gacgccagca aagccgaccg tgaactgaac tggcgcgtaa
     8221 cgcgcacact cgatgaaatg gcgcaggaca cctggcactg gcagtcacgc catccacagg
     8281 gatatcccga ttaaggaacg accatgacgc aatttaatcc cgttgatcat ccacatcgcc
     8341 gctacaaccc gctcaccggg caatggattc tggtttcacc gcaccgcgct aagcgcccct
     8401 ggcagggggc gcaggaaacg ccagccaaac aggtgttacc tgcgcacgat ccagattgct
     8461 tcctctgcgc aggtaatgtg cgggtgacag gcgataaaaa ccacgattac accgggactt
     8521 acgttttcac taatgacttt gcggccttga tgtctgacac gccagatgcg ccagaaagca
     8581 acgatccttt gatgcgttgc cagagcgcgc gcggtaccag ccgggtgatc tgcttttcac
     8641 cggatcacag taaaacgttg ccagaactga gcgttgcggc attgacggaa atcgtcaaaa
     8701 cctggcagga gcaaaccgca gagctgggaa aaacgtaccc gtgggtgcag gtctttgaaa
     8761 acaaaggtgc ggcgatgggc tgctctaacc cgcatccgca cggacagatt tgggcaaata
     8821 gcttcctgcc taacgaagct gagcgcgaag accgcctgca aaaagaatat tttgccgaac
     8881 agaaatcacc aatgctggtg gattatgttc agcgcgagct ggcagacggt agccgtaccg
     8941 ttgtcgaaac cgaacactgg ttagccgttg tgccttactg ggctgcctgg ccgttcgaaa
     9001 cgctactgct gcccaaagcc cacgttttgc ggatcaccga tttgaccgac gcccagcgca
     9061 gcgatttggc gctggcgttg aaaaagctga ccagtcgtta tgacaacctc ttccagtgct
     9121 ccttccccta ctctatgggc tggcacggcg caccatttaa tggcgaagag aatcaacact
     9181 ggcagctgca cgcgcacttt tatccgcctc tgctgcgctc cgccaccgta cgtaaattta
     9241 tggttggtta tgaaatgctg gcagagaccc agcgagacct gaccgcagaa caggcagcag
     9301 agcgtttgcg cgcagtcagc gatatccatt ttcgcgaatc cggagtgtaa gaaatgagtc
     9361 tgaaagaaaa aacacaatct ctgtttgcca acgcatttgg ctaccctgcc actcacacca
     9421 ttcaggcgcc tggccgcgtg aatttgattg gtgaacacac ggactacaac gacggtttcg
     9481 ttctgccctg cgcgattgat tatcaaaccg tgatcagctg tgcaccacgc gatgaccgta
     9541 aagttcgcgt gatggcagcc gattatgaaa atcagctcga cgagttttcc ctcaatgcgc
     9601 ccattgtcgc gcatgaaaac tatcaatggg cgaactacgt tcgtggcgtg gtgaaacatc
     9661 tgcaactgcg taacaacagc ttcggcggtg tggacatggt gatcagcggc aatgtgccgc
     9721 agggtgccgg gttaagttct tccgcttcac tggaagtcgc ggtcggaacc gtattgcagc
     9781 agctttatca tctgccgctg gacggcgcac aaatcgcgct taacggtcag gaagcagaaa
     9841 accagtttgt tggctgtaac tgcgggatca tggatcagct aatttccgca ctcggcaaga
     9901 aagatcatgc cttgctgatt gactgtcgtt cactggggac caaagcagtt tccatgccga
     9961 aaggtgtggc tgtcgtcatc atcaacagta acttcaaacg taccctggtt ggcagcgaat
    10021 acaacacccg tcgtgaacag tgcgaaaccg gtgcgcgttt cttccagcag ccagccctgc
    10081 gcgatgtcac cattgaagag ttcaacgctg ttgcacatga gctggaccca atcgtggcga
    10141 aacgcgtgcg gcatatcctg actgaaaacg cccgcaccgt tgaagctgcc agcgcgctgg
    10201 agcagggcga cctgaaacgt atgggcgagt tgatggcgga gtctcatgcc tctatgcgcg
    10261 atgatttcga aatcaccgtg ccgcaaattg acactctggc agaaatcgtc aaagctgtga
    10321 ttggcgacaa aggtggcgta cgcatgaccg gcggcggatt tggcggctgt atcgtcgcgt
    10381 tgatcccgga agagctggtg cctgccgtac agcaagctgt cgctgaacaa tatgaagcaa
    10441 aaacaggtat taaagagact ttttacgttt gtaaaccatc acaaggagca ggacagtgct
    10501 gaacgaaact cccgcactgg cacccgatgg tcagccgtac cgactgttaa ctttgcgtaa
    10561 caacgcaggg atggtagtca cgctgatgga ctggggtgcg actttacttt cagcccgtat
    10621 tccgctttcc gatggcagcg tccgcgaggc gctgctcggc tgtgccagcc cggaatgcta
    10681 tcaggatcag gccgcgtttc tgggggcctc tattggtcgt tatgccaacc gcatcgccaa
    10741 tagccgttat acctttgacg gtgaaaccgt gaccctttcg ccaagtcagg gcgttaacca
    10801 gctgcacggc ggcccggaag ggttcgacaa acgtcgctgg cagattgtga accagaacga
    10861 tcgtcaggtg ctgtttgccc tgagttcaga tgatggcgat cagggcttcc cgggtaatct
    10921 cggcgcaacg gtgcaatatc gtctgaccga cgataaccgt atctccatta cttatcgcgc
    10981 cacagttgat aaaccttgcc cggtgaatat gactaatcac gtctatttca atcttgacgg
    11041 cgagcagtct gacgtgcgca atcacaagtt gcagattctg gcggacgaat atctgccggt
    11101 tgatgaaggc ggcattccgc acgacggcct gaaatctgtc gccggaacgt cttttgattt
    11161 ccgcagcgcc aaaatcatcg ccagtgagtt tcttgccgac gacgatcagc gcaaagtgaa
    11221 aggttacgat cacgcattct tgttacaggc caaaggcgat ggcaagaaag tggcggcgca
    11281 tgtctggtca gcagatgaaa aattgcagct gaaggtctac accaccgctc cggctctgca
    11341 attctactcc ggcaacttcc tcggcggcac accgtcgcgg ggaacagaac cttacgccga
    11401 ctggcaaggg ctggcgttgg aaagcgagtt tctgccggac agcccgaacc accctgaatg
    11461 gccgcaaccg gactgcttcc tgcgtcctgg cgaagagtat tccagcctga cggaatatca
    11521 gtttattgct gagtaatgtt aagccctcgt catgagggct ttatctcata ttgctcaaat
    11581 caccagcaaa caccgacata tttgcaactc aatattcaca acaaccttac actgcgccac
    11641 tattttcgct atggttatgc gtaagcattg ctgttgcttc gtcgcggcaa tataatgaga
    11701 attattatca ttaaaagatg atttgaggag taagtatatg gctgtaacta agctggttct
    11761 ggttcgtcat ggcgaaagtc agtggaacaa agaaaaccgt ttcaccggtt ggtacgacgt
    11821 ggatctgtct gagaaaggcg taagcgaagc aaaagcagca ggtaagctgc tgaaagagga
    11881 aggttacagc tttgactttg cttacacttc tgtgctgaaa cgcgctatcc ataccctgtg
    11941 gaatgtgctg gacgaactgg atcaggcatg gctgcccgtt gagaaatcct ggaaactgaa
    12001 cgaacgtcac tacggtgcgt tgcagggtct gaacaaagcg gaaactgctg aaaagtatgg
    12061 cgacgagcag gtgaaacagt ggcgtcgtgg ttttgcagtg actccgccgg aactgactaa
    12121 agatgatgag cgttatccgg gtcacgatcc gcgttacgcg aaactgagcg agaaagaact
    12181 gccgctgacg gaaagcctgg cgctgaccat tgaccgcgtg atcccttact ggaatgaaac
    12241 tattctaccg cgtatgaaga gcggtgagcg cgtaatcatc gccgcacacg gtaactccct
    12301 gcgtgcgctg gtgaaatatc ttgataacat gagcgaagaa gagattcttg agctgaatat
    12361 cccgactggc gtgccgctgg tgtatgagtt cgacgagaat ttcaaaccgc tgaaacgcta
    12421 ttatctgggt aatgctgacg agatcgcagc gaaagcagcg gcggttgcaa accagggtaa
    12481 agcgaagtaa acgtcattcg tttaaaatga gaaagccgac tgcaagtgag tcggcttttt
    12541 tgttgctaac aatggagcac attgcctgat gcgacgcttc gcgacttatc aggcctgtgg
    12601 tgattcatcg gatacgccac tctgacggcg catccgacaa ttaaacctta cccgcgacgc
    12661 gcttttactg cattcgccag ttgacgtaac agagcatcgg tatcttccca gccgatgcag
    12721 gcatcggtga tgctcttacc gtaggccagc ggctccccgc tctcgaggct ctgattgcct
    12781 tccaccagat ggctttccac catcacgcca ataatggcct tttcgccacc ggcaatctgc
    12841 tggcaaacgt cagcacaaac atccatctgc tttttgaatt gtttggacga gttagcatgg
    12901 ctgaaatcga tcatcacctg tgctggcagg cctgctttgt tcagcccttc tttcacttca
    12961 gcaacgtgct tcgcgctgta gttaggctct ttaccgccgc gcagaatgat atggcaatcg
    13021 ccgttaccgc tggtattcac aatcgccgaa tgcccccatt tcgttacgga caggaagcag
    13081 tgcggcgcac cggcggcatt aatggcatcg atagccactt taatcgtacc gtcggtgcca
    13141 tttttgaagc cgactggaca agaaagccct gatgccagtt cgcggtgcac ctgcgattcg
    13201 gtggtacgtg cgccaattgc gccccagctc atcaggtcag cgagatattg tggggtgatc
    13261 atatcgagaa actcacccgc cgctggcaga ccgctgtcgt taatatcaag cagcaattta
    13321 cgggctatac gaagaccgtc gttgatctgg aagctgttat ccatatgcgg atcgttaatc
    13381 agccctttcc agcccaccgt ggtacgcggc ttttcaaaat agacgcgcat tacgatttcc
    13441 agctcatctt tcagctcttc acgcagcgcc agcaagcgag tggcatactc tttagccgcg
    13501 acaggatcat gaattgagca cgggccaatc accaccaaca ggcgatcatc attacctttc
    13561 aggatcttat ggatcgcttt tcgggcatgg gcaaccgtat tcgcggcatt ttcagtagcg
    13621 gggaattttt ccagcaatgc gacaggagga agtaactctt tgatttcttt gatgcgtaaa
    13681 tcgtcgttct gataattcat gtctgttcca gtgttgccat acttatctta gtgaatgcaa
    13741 tacttccaat ctatatcttc cgtcagaatg tgtaaacggg gttttacatt atgaacggat
    13801 taatcctgga attagcaaga aaaacgccag attgtcgcga aaaacgagat ctctcctaca
    13861 atttctaact gtaactcctt tgtttgttaa ttatttcaag attctctgct gcgtttcata
    13921 aactggctga aatcttaaac caatgcctta tattcacctg caaatgcact gttggaagag
    13981 gttatccgac ataacgacca taacaggagc atcctatgaa aatgacaaaa ctggccacat
    14041 tatttctgac tgccactcta agccttgcca gcggtgccgc actggctgcc gatagcggag
    14101 cgcaaactaa taacggccag gcaaacgccg caactgatgc gggccaggta gcccctgacg
    14161 cccgtgaaaa tgtcgcgcca aataacgtcg acaataacgg ggtaaatacc ggttctggcg
    14221 gcacaatgct gcattcggat ggttcttcaa tgaacaatga cggaatgacc aaagatgaag
    14281 agcacaagaa caccatgtgc aaagatggtc gatgtccgga tatcaataaa aaggtacaaa
    14341 ccggtgatgg catcaataat gatgtcgata ccaaaaccga cggtaccacg cagtaaccct
    14401 gcaaaatagc caataatggg agagcggagt ctctcccttt taatttttca cttctaaaag
    14461 cagtatgatg tcataaagtt aagtgagaat aattaaggga tgactcgtca tggcgcactc
    14521 acactcacac acgtcctcac acctgccaga agataataat gctcgtcgct tgttgtatgc
    14581 tttcggcgtg actgccgggt ttatgctggt agaagtcgtt ggtggttttc tttctggttc
    14641 tctggcattg ctggccgatg cgggtcatat gttgaccgat actgccgccc tgcttttcgc
    14701 cctactcgcc gtgcaatttt cacgtcgtcc tcccaccatt cgccacactt tcggttggct
    14761 aagactgacc acgctcgcgg cgtttgtgaa tgccatcgcc ctcgtggtaa ttaccatttt
    14821 gattgtctgg gaggcgatag aacgcttccg cacgccgcgt ccggtcgagg gcggcatgat
    14881 gatggcaatt gccgtggctg ggttgctggc aaatatactt tctttctggt tacttcatca
    14941 cggcagcgaa gagaaaaacc tcaacgtacg agcagcggca ttgcatgtgc tcggggacct
    15001 gttgggttcg gttggggcta ttattgccgc gttgattatt atctggaccg gctggacacc
    15061 tgctgacccc attctctcga tagtggtgtc gcttctggtt ctgcgtagcg cgtggcgact
    15121 gttgaaagat agcgtgaatg aattacttga aggtgcaccg gtatcgctgg atatcgctga
    15181 actgaagcgt cgtatgtgcc gggaaatccc ggaagtccgc aatgtgcacc atgtacatgt
    15241 atggatggta ggtgagaagc cggtgatgac gctgcatgtg caggtgatcc cgccacacgc
    15301 tcacgatgcc ttgttggatc agatccaaca ctacctgatg gatcactatc agattgagca
    15361 cgccaccatt cagatggaat atcaaccttg tcatgggcca gactgccacc tgaatgaagg
    15421 ggtgtctggc cattcacatc atcaccatta atgggacagc gcgcatgagc ctctttcacg
    15481 tgcgctgttg atccacatcc gactgccgtt gagcgcaata aaggtcagga tgatgtactc
    15541 cagagacatg gcgtaaacgc cctgaagtgc aaaaataacg acgctaatca cgttaataat
    15601 cacccacaac agccagtttt ccacatactt acgcgtcatc agaagcattg ccacgataga
    15661 taacaccatc atgcatgaat cccagaacgg gaaagcgtcc ggttgcagtt caggcatcac
    15721 aacctgtaat cctaatgctt gcatgatcat gactgccacg cgggtcaaaa atgcaaacac
    15781 cggattgata aataccgtca tcagaccaat cgaaacaacg caaaccgcca accagctgag
    15841 cgccttcggc aatggtaacc agtgaatttt caactccgcc tcgttctgac tggtttgtcg
    15901 cgaccacgca taccaaccgt aaatattcgc ggcaaagaaa aacacctgta atagcaggct
    15961 ggcatacagc tgaatctgaa agaaaataat gccaaacaag gtgacgttga tcaggccaaa
    16021 gaagtagttg ctgatcttct ccagactggc aaggccaata cacagcaacc cggcgatcgt
    16081 gcctaccgct tcgatccatg agagatcata accgcctgct cctattggta tatgtaccag
    16141 gatattctgc acactaaaaa aatccatctt tcccccaaag cgtaacgtta cgccttatta
    16201 ttcgttatcc acgtagtgta gccgcaaaat ccagcatacg attgagcggc accagcgccc
    16261 tctctcgcag cctttcatca acatgaacct cgtgattgct tccttcctgt tctaatgcct
    16321 ctgcgatggc ctgaaggcca ttcatggcca tccacggaca atgcgcgcag ctgcggcagg
    16381 ttgcaccctc acctgcggtt ggtgcttcca gtaactcttt atctggcacc gcctgctgca
    16441 ttttgtagaa aataccccga tcggttgcca caataagcct ctgatgtggc aatgttttcg
    16501 cagcagcgat cagttgactg gtggaaccga ccgcatccgc catatcgaca atagcttgtg
    16561 gtgattctgg atgcaccagt atggcagcat ccgggtattc ttcttgcaag cgggttaacg
    16621 cctgagtctt aaattcatca tgcacaatac aggcaccctg ccagcataga atgtctccac
    16681 ccgtctgttt ttgcacgtaa cgccccagat gtttgtcggg tgcccagatg attttttcac
    16741 ccaaactatc aagatgatca ataagttcga cggcaatgct tgaagttacc acccaatctg
    16801 cgcgcgcttt taccgcagca gaagtgttgg cgtagacgac gacagtacga tcgggatggg
    16861 catcgcaaaa tgcgttaaat tcttcaacag ggcagccgag atccagtgaa cattcagcct
    16921 gaagtgtcgg catcagaatt gttttttccg gactgagaat tttggcggtt tctcccataa
    16981 atctcacccc agcgactaac aaagtagaag cgggatgctt tgcaccgaag cgcgccattt
    17041 ccagagaatc agaaatacag ccaccggttt cttctgccag ttgttgaatt tcgggatcgg
    17101 tatagtagtg ggcaaccatc accgcattac gttcttttag cagacgtttt atcttctcgc
    17161 ggtaatacgc tttttcatca atgcttaacg gcgtcggctt cggggggaaa ggataaatcg
    17221 ccgtgtctgg atcaaacatt acgctcatct taccatctcg ttttacaggc ttaacgttaa
    17281 aaccgacatt agcgtaatat tcgctgttat cggtgtaatt tgttttatat gctaaacaag
    17341 atagtcgaat ggtggggaaa agtcactcga attacgccta attccaccga cgtagagttg
    17401 cttgcacagt aagctctgac gatgtcatca ggtttacaaa gtgaaattga ggatgcctg
//
LOCUS       DADLFA010000082        17331 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000082 DADLFA010000000
VERSION     DADLFA010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17331
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.082"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="JQH65_002285"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="JQH65_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAY6139682.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="JQH65_002286"
     CDS             2219..2767
                     /locus_tag="JQH65_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HAY6139683.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="JQH65_002287"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="JQH65_002287"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HAY6139684.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3663..4853)
                     /locus_tag="JQH65_002288"
     CDS             complement(3663..4853)
                     /locus_tag="JQH65_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HAY6139685.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARMAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(5038..6126)
                     /gene="ompF"
                     /locus_tag="JQH65_002289"
     CDS             complement(5038..6126)
                     /gene="ompF"
                     /locus_tag="JQH65_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HAY6139686.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="JQH65_002290"
     CDS             complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="JQH65_002290"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAY6139687.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8284..9486)
                     /gene="pncB"
                     /locus_tag="JQH65_002291"
     CDS             complement(8284..9486)
                     /gene="pncB"
                     /locus_tag="JQH65_002291"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAY6139688.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYKKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9752..12364
                     /gene="pepN"
                     /locus_tag="JQH65_002292"
     CDS             9752..12364
                     /gene="pepN"
                     /locus_tag="JQH65_002292"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HAY6139689.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPEHFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVISLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQFHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(12407..13158)
                     /gene="ssuB"
                     /locus_tag="JQH65_002293"
                     /pseudo
     CDS             complement(12407..13158)
                     /gene="ssuB"
                     /locus_tag="JQH65_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
     gene            complement(13155..13892)
                     /gene="ssuC"
                     /locus_tag="JQH65_002294"
                     /pseudo
     CDS             complement(13155..13892)
                     /gene="ssuC"
                     /locus_tag="JQH65_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001714028.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
     gene            complement(13957..15102)
                     /gene="ssuD"
                     /locus_tag="JQH65_002295"
                     /pseudo
     CDS             complement(13957..15102)
                     /gene="ssuD"
                     /locus_tag="JQH65_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
     gene            complement(15099..16058)
                     /locus_tag="JQH65_002296"
     CDS             complement(15099..16058)
                     /locus_tag="JQH65_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY6139690.1"
                     /translation="MRNIIKLALVGLLSVSTIAVAAESSPEALCIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGMRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFVQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(16051..16626)
                     /gene="ssuE"
                     /locus_tag="JQH65_002297"
     CDS             complement(16051..16626)
                     /gene="ssuE"
                     /locus_tag="JQH65_002297"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HAY6139691.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFVP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHRPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            complement(17083..17322)
                     /locus_tag="JQH65_002298"
                     /pseudo
     CDS             complement(17083..17322)
                     /locus_tag="JQH65_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706859.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4367 a         4439 c         4393 g         4132 t
ORIGIN      
        1 taacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgccgg tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgcgac gtagtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccggtcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcacttg caacgccgcc gctctcggtg attaaccaat ggcagctggc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gcgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggggcaga
     1201 tggtgctatt ggcccggcaa cacgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggct ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccggcct attcgctggt ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcccttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt acctccaact ctggcacgca aagatattct
     1501 gccaaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgtgg
     1561 ctggaacagc agagaagcga ttgacccatg gcaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg ttccgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggctg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atggtcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca gttgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga cctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttccatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattacccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagcaa tcgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attaatctgg tgtgaacaaa cccgtctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata ggaaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc cacacttggt tatgaacgcc tgcataatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aggcacataa aaaagcccgc ttttaatgcg ggcctggatt
     3481 tctggcaaag tgcgctttgt ttatgccgga tgcggcacga gcgccttatc cggcctacaa
     3541 aatcgtgcaa attcaaaata ttgcagggga cgcgtaggcc tgataagcgt agcgcatcag
     3601 gcaatgttgc gtttgtcatc agtctcagcc cgcttttcag cgggcttcat tgtttttaat
     3661 gcttacagca ctgccacaat cgcttcgcac agcggagcca tgttatctgg tgtcatcccg
     3721 gccacattta cgcgaccaga agcaaccgca tatacgccaa actcttcgcg cagacgcagc
     3781 acttgttctt ttgtcaggcc actgaaggag aacatgccgt tctgtttgat gataaagctg
     3841 aagtcgcggt ttgcgccttt ttcctgcagc gtattgacga acaactgacg catacgctga
     3901 atacgctggc gcatatcagt cagctcttgt tcccaaatcg cacgtaacgc atcgttgctc
     3961 aggatggtgg caacaacaga agcgccgtgt gctggtgggt tagagtagtt agcgcgaatc
     4021 gccgctttca tttggctgaa tgcgcgatca acggtttcac tgtcggcagc aaccagagta
     4081 caagcgccaa cacgctcgtt gtacaggcca aagtttttag agtaggaact ggcaacaatc
     4141 agctctttat gcatagccgc gaaagcgcgc agtccttcag catcttcttc cagaccacgg
     4201 gcaaaaccct ggtaagcgaa gtcaaacagc ggtaaccagc ctttctcaac ggagagttgt
     4261 gccagtgtct gccattgttc cagcgtaggg tcgataccgg ttgggttatg gcagcagcca
     4321 tggaacagca ctacgtcgcc agcctgagct tcattcaggc tgttaatcag tgcatcgaag
     4381 tcaagagtgt gattttccgc atcataataa gcgtattcac gaacttccag acctgcagag
     4441 ttaaagacgc tcttatggtt cggccagctt gggttgctca cccacactcg cttaacgctg
     4501 gtattttttg ccaggaaatc ggcagctacg cgtagtgcgc cagtgccccc cggagtctgt
     4561 gccatgcgag cacgtttgtc attgatcagg gcgctacctt taccaaacag cagttcctga
     4621 gtgcagcgac caaattcagg gatgccgtca atgccgaggt aatttttggt ggtttcattt
     4681 tcgagcagat actgttcagc ctttttcacg ctggtcagta ccggggtttt gcccgtctca
     4741 tctttataga caccaatccc gaggttaatt ttgccgggac gttcatcggc acgaaacaga
     4801 tcggccaggc ccagaatcgg gtcggcagga gcggcggtaa tgttctcaaa catgacgagg
     4861 ttccattatg gttacagaag ggaagtccgc tatcagggta acgggagatt tacaaaattc
     4921 caactattac tgatgaaaac gcaggctgtt tttgcaagac gtgagattgc tctggaaggt
     4981 ataaaaaaaa caggaccaaa gtcctgtttt ttcggcattt aacaaagagg tgtgctatta
     5041 gaactggtaa acgataccca cagcaacggt gtcgtctgaa cctacgccca gtttgttatc
     5101 agaatcgatc tggttgatga tgtagtcaac ataggtggac atgtttttgt tgaagtagta
     5161 ggttgcgccc acttcaaagt agttcaccag atcaacatca ccgatacctt ctacgtcttt
     5221 cgctttagat ttggtgtaag cgatggacgg acgcagaccg aaatcgaact ggtattgcgc
     5281 aactaacaga acgtcttgcg ttttgttggc gaagccgctg gtgtttgtaa atttattagt
     5341 gatcggcgta gcgttacggg tttcaccgta gttcgctgcc aggtagatgt tgttcgcgtc
     5401 gtacttcaga ccagtagccc actgttcagc ttttttaccg ttgccaagag gttgagcttc
     5461 ttgcaggttg gtacggtcag ctgcaccata agcaccaacg ataccaaagc cttcgtattc
     5521 gtagctgata gaaccgccaa caccgtcgcc gttagaacgg cgtgcagtgt cacgctcgtt
     5581 tttacccagg tactgaacag cgaagttcag gccatcaacc agaccaaaga agttggagtt
     5641 acgataggta gcaacgccgc caacacgacc aacgaagaag tcatcgctgt atgcagtatc
     5701 accaccaaat tctggcagca tatcggtgta acccagtgca tcataaacca caccgtagtt
     5761 acggccgtaa tcgaaagaac caacgtcagc gtatttaaga cccgcgaatg ccagacgcgt
     5821 tttgttacca gtttgagcgt cagcgccttc agagttgtta ccttggaagt tatattccca
     5881 ctgaccataa ccggtcagat cggaattgat ttgagtttcc cctttaaaac caagacgggc
     5941 ataggtcatg tcgccattgc caccgtaact gttttcaccg ttacccttgg aaaaataatg
     6001 cagaccaaca gctttaccgt acagatctac tttgttgcca tctttgttat agatttctgc
     6061 agcgtttgca gtacctgcta ctaacagagc agggacgatc actgccagaa tattgcgctt
     6121 catcattatt tattaccctc atggtttttt ttatgacacc tgccactgcc gtcaataagt
     6181 tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag
     6241 gcatctttcc attcaaacta acgtttcgct accgtgaaag tgctgcaaag ataaagattt
     6301 agtttcaaaa agaaaaaata tgtaaccaaa agtaaaaatt aaggaacttt gtgaacaccg
     6361 tcatatttcc atagaaacgt gatgatattt acagcaattt ttatctattt atatgattta
     6421 cttatattta aattaactaa gcggaaattt tgtttctgat ggaaacttta tcgacctggc
     6481 acaaaatcct ctttcagaca tccagaatgc caaaaaatag tatgaaaatt gtgctatcag
     6541 ctaagaaaaa actaatccgc aataagaata cgggttattt tttgtgcgta aatgttgcaa
     6601 tgtaatattc cgtaacagga tgatcgttat ggcggggttg caaatagatt gcttgactta
     6661 acaaacagat aacttgacag aaaggataaa aaagagccag caggcgctgg ctctttgagg
     6721 aagaaaatca gaagctggcg ttgcgcggag tacgtgggaa cggaatcaca tcacgcacgt
     6781 tttgcacgcc agttacgtaa gcaatcaggc gttcaaaacc aagaccgaaa cctgaatgcg
     6841 gaacagtacc gtagcgacgc agatcgcgat accaccagta atcttcttta ttcaggccca
     6901 tttccagcat acgctcgtcc agaacgtcca gacgttcttc acgctgggag cctccaatga
     6961 tctcaccgat gcccggagcc agaacgtcca tagccgcaac ggttttaccg tcttcgttga
     7021 ggcgcatata gaacgcttta atatctttcg gatagttttt aaccactacc ggtgctttaa
     7081 agtgttcttc cgccagataa cgctcatgct cagaagagag atcgacgccc cagtaaaccg
     7141 ggttttcaaa cttcctgccg cagttttcga gaatggtcac tgcgtcggtg taatccacct
     7201 gcgcgaaatc ggcctcaatg aagcgttcca gacgtgaaac ggcatcttta tctacgcgtt
     7261 cagcgaagaa tttcatgtcg tctgcgcgtt cttcgagaac cgctttgaag acatatttca
     7321 gcatggcttc agccagaccc gcgatatcgt tcaggttagc aaacgccact tccggctcca
     7381 gcatccagaa ttccgccagg tgacggctgg tgttggagtt ttcagcacgg aaagtcgggc
     7441 cgaaggtata aattttggat aatgcgcaag cgtaggtttc gccgttcaac tggccagata
     7501 cggtcaggaa agactcttta ccaaagaagt ctttgtcgaa atccactttg ccctgatcgt
     7561 tacgcggcag gttttccaga tccagcgtag aaacgcggaa catttcgcct gcaccttcgg
     7621 tatcagatgc ggtaatcagt ggcgttgaaa cccagaagaa tccctgctcg ttaaagaagc
     7681 gatgcagcgc ctgcgccagc gtatggcgaa cgcgcgcgac ggcaccaatc aggtttgtgc
     7741 gcggacgcag gtgagcgact tcacgcagat actcaatgct gtggcgtttt gccgccatcg
     7801 ggtaagtgtc tggatcttca acccaaccag caacttcaac tttgctggcc tgaatttcaa
     7861 attgttgccc ctggcccggc gacgccacga ctttacccgt cacaatgacc gagcagccgg
     7921 tggtcagacg caggacgtct tcattgtaat tgggcagaga attattgatg acagcctgta
     7981 caggatcaaa gcaggaaccg tcataaacgg cgaggaagga gatgccagct tttgaatctc
     8041 ggcgggtacg tacccatccg cgtacggtga cttcgctgtc aacggctacg cggccctgga
     8101 gtacgtcggc tacaggcaca acgctcataa tattctctct gttaatagtc ggaaaaaata
     8161 aacacttgtc cacccgtaaa tggggggata cctatgttac ctggcatctg caatcagaca
     8221 agcagaattc gcaaatgcag cgaaagattt aaaaaaaggg agccgattag ctccctgatg
     8281 atattaactg gcttttttaa tatgcggaag gtcgaacgct ttgcgcagcg cccgaacaaa
     8341 cgctttatca tggcagatag ttttgccagg gctgtcagaa agtttcgcca ccggtttacc
     8401 gttacactct accaacttaa tcacaatatt caggggtttt acctggggga tatcgcaggt
     8461 caggcgagtc ccaataccaa aacttaattg cacgcgggaa gagaagtggc ggtatagctc
     8521 aaccgctttg cgtaaatcca gattgtcaga gaaaaccagc gttttactct gtggatcaat
     8581 tcccagcttt ttataatgtg caatggcttt ttcaccccat tcaaccgggt cgccagagtc
     8641 atgacgcagg ccctgatacc gactggcgaa ctcgacaccg aaatcacgca ggaaagcatc
     8701 catagtgatg cagtcggtta atgcaatgcc aagttggtcg ggatattctt ccagccaggc
     8761 agcaagtgca gctcgctggc tgttggctag gtccgggctg atttgctgat gtgcctggaa
     8821 ccattcgtgc gcctgtgttc ccatcggcgt gagggaaagc cgacgcgcca gatcgtagtt
     8881 gctggtgccc acgaaccagg attcctgttg cagacgctta acgatggttt cttgtacttc
     8941 gcgagaaaaa cggcgacggg tgccaaaatc catcagatgg aagcgcgaca tatcaagacc
     9001 ggcggttaac gccgagaagt cgactaattt gctttccagc gtgtcgaggg cttgcgcaac
     9061 gtcggcctgc ggtgagcgat agcgatgtac catttcactg ataaccgcca gcaaaggaac
     9121 ttcccagagg atgacttcac gccacggacc gcttaagcga atatccagct tgccattatc
     9181 gttggacacg gtgacttgtt ccgggttaaa gcggaattcg cgtaaccagt taagatagtc
     9241 ggccttaaag aaaggcaggg cagagagcca ctgatattca tcatcctgca ggcgcaggtg
     9301 ctgcatcgcc tgaatctgtt cacgaatagc atcggcataa atacccagca gatcgtcacc
     9361 tcggcaacga aactccgccg cgacatgcac atcgtaatag tgatgaaaca cggcttgctg
     9421 catatgcaac ttataagcat ctgtatccag caacgagtgc agaacaggag aagcgaattg
     9481 tgtcataggt gcgcagtagc atcctcgtac aggagcgttt agtacaataa acatcttcag
     9541 gaactgctgg agtatacctt gtttcgcaat ttattgaacc ccgatcacac catatgccac
     9601 ctttctggtc gatggcatta cgcggtgtat gttataaaaa tgtagcaata aaggcgtttg
     9661 tacctgaaaa gatgaagatt ctgcatagcg cgatttacgc aacaggaata gactgaacac
     9721 cagactctat aaaagatgct aaaggttatt tatgactcaa cagccacaag ccaaataccg
     9781 tcacgattat cgtgcgccgg attaccagat tactgatatt gacttgacct ttgacctcga
     9841 cgcgcaaaag acggtcgtta ccgcggtcag ccaggctgtc cgtcatggtg catcagatgc
     9901 tccgcttcgt ctcaacggcg aagacctcaa actggtttct gttcatatta atgatgagcc
     9961 gtggaccgcc tggaaagaag aagagggcgc actggtcatc agtaatttgc cggagcattt
    10021 tacgcttaag atcgttaatg aaataagccc ggcggcgaat accgcgctgg aagggcttta
    10081 tcagtcaggc gatgcgcttt gcacccagtg tgaagccgaa ggtttccgcc atattacgta
    10141 ttatctcgac cgcccggacg tgctggcgcg ttttaccacc aaaattattg ccgataaaac
    10201 caaatatccc ttcctgcttt ccaacggtaa ccgcgttgcg caaggtgaac tggaaaacgg
    10261 acgccattgg gtacagtggc aggacccgtt cccgaaaccg tgctacctgt ttgcgctggt
    10321 ggcaggcgac tttgatgtac tgcgcgacac ctttaccacg cgttctggtc gcgaagtggc
    10381 gctggagctg tacgtcgatc gcggcaacct tgatcgcgcg ccgtgggcga tgacctcgct
    10441 gaaaaactca atgaaatggg atgaagaacg cttcggcctg gagtatgacc tcgacatcta
    10501 tatgatcgtc gcggtggatt tcttcaatat gggcgcaatg gagaataagg gtttgaatat
    10561 ctttaactcc aaatatgtgc tggcccgcac cgacaccgcc actgacaaag attacctcga
    10621 tattgaacgc gttatcggcc atgaatattt ccataactgg accggtaacc gagtcacctg
    10681 ccgcgactgg ttccagctca gcctgaaaga aggtttaacc gtcttccgcg atcaggaatt
    10741 cagctctgac cttggttccc gtgcagttaa ccgcatcaat aatgtgcgca ccatgcgcgg
    10801 attgcagttt gcggaagacg ccagcccgat ggcgcacccg atccgcccgg acatggtcat
    10861 tgagatgaac aacttctata ccctgaccgt ttacgagaag ggcgcggaag tgatccgtat
    10921 gatccacacc ctgctgggcg aagaaaactt ccagaaaggg atgcaactct atttcgagcg
    10981 ccatgatggc agcgcggcaa cctgcgacga ctttgtgcag gcgatggaag atgcgtcgaa
    11041 tgtcgatctc tcccatttcc gccgttggta cagccagtcc ggcacgccga ttgtgaccgt
    11101 caaagacgac tacaatccgg aaaccgagca gtacaccctg accatcagcc agcgcacgcc
    11161 agccacgccg gatcaggcag aaaaacagcc gctgcatatt ccgtttgcca tcgaattgta
    11221 tgacaacgaa ggcaaagtga tctcgttgca gaaaggcggt catccggtga attccgtgct
    11281 gaacgtcacc caggcggaac agacctttgt ttttgataat gtctacttcc agccggtgcc
    11341 tgcgctgctg tgcgaattct ctgcgccagt gaaactggaa tataagtgga gcgatcagca
    11401 actgaccttc ctgatgcgtc atgcgcgtaa tgatttctcc cgctgggatg cggcacaaag
    11461 cctgctggca acctacatca agctgaacgt cgcccgacat cagcaagggc agccgctgtc
    11521 tctgccggta catgtggctg acgctttccg cgcagtgctg ctcgatgaga agattgatcc
    11581 ggcgctggcg gcagaaatcc tgacgctgcc ttctgtcaat gaaatggctg aactgttcga
    11641 tatcatcgac ccgattgcta ttgccgaagt acgcgaagca ctcactcgta ctctggcgac
    11701 tgaactggcg gatgagctgc tggctattta caacgcgaat taccagagcg agtaccgtgt
    11761 tgagcatgaa gatattgcga aacgcactct gcgtaatgcc tgcctgcgct tccttgcttt
    11821 tggtgaaacg catctggctg acgtgctggt gagcaagcag ttccacgaag cgaacaatat
    11881 gaccgatgcg ctggcggcgc tttctgcggc ggttgccgca cagctgccat gccgtgatgc
    11941 gctgatgcag gaatacgacg ataagtggca tcaggacggt ctggtgatgg ataaatggtt
    12001 tatcctgcaa gccaccagcc cggcggcgaa tgtgctggag acggtgcgcg gtctgttgca
    12061 gcatcgctca tttaccatga gcaacccgaa ccgtattcgt tcgttgattg gcgcgtttgc
    12121 gggcagcaat ccggcagcgt tccatgccga agatgggagc ggttaccagt tcctggtgga
    12181 aatgcttacc gacctcaaca gccgtaaccc gcaggtggct tcacgtctga ttgaaccgct
    12241 gattcgcctg aaacgttatg atgccaaacg tcaggagaaa atgcgcgcgg cgctggaaca
    12301 gttgaaaggg ctggaaaatc tctctggcga tctgtacgag aagatcacca aagcactggc
    12361 ttgataaata accgaatggc ggcaatagcg ccgccattcg gggaatttac ccctgttttc
    12421 tcaggcgaat ttcagattca ccacgctgca tcaccctttg caacacttcc gattccagct
    12481 ccgctaatcg caccgaccct aatcgacgcg gaggggggat atccactgtc aaatccagac
    12541 caatttttcc ctcttcaatt aataacaccc ggtcagccat tgctaccgct tcgctcacat
    12601 catgcgtcac caacagtacg gtaaagccgt gctcctgcca aagtgacaca atcaaatcct
    12661 gcatctcgag tcgcgttaag gcgtccagtg ccccgagcgg ttcatcaagc aacaataaac
    12721 ccggtcgatg aatcaacgct cttgccagca ccactcgctg tttctgcccg cctgaaagtg
    12781 cggcaggcca ttccccggcg cgattctcca gtcctaccgc agccaacgcc cgacgcgcgg
    12841 catcgcgcca ctggccttta aggcctaacc caacgttatc aatcaccgat ttccacggta
    12901 gcagacgcgc atcctgaaac atcattcgcg tgtcttcctg aatttcagcc agcggtgtgg
    12961 tgcctgccaa cacatcgcct gcggttggcg tttccagccc ggccagcagg cgcagcaggg
    13021 tacttttgcc accaccgcca caaactgacc tgccggaata tgtaaatcca gttggttcag
    13081 gacgatattt tccgcgtaat gtttgcttac tgcattgagc aacaatggtg tgccctggtt
    13141 cagacgagca gtattcatac cgtggcctcc ttcaaatgat aagccgggtt ccagcgcaac
    13201 cagaggcgct ctaacagctg cgcgctgacg tcagcaagtt tgccgagcag ggcgtaaaga
    13261 ataatagcga ctaccaccac gtccgtttgc aaaaactctc gcgcattcat cgccagataa
    13321 ccaatgccgg aattggcaga aatggtttcg gcaacaatca gcgtcagcca catcaggccc
    13381 aacgcaaaac gcacgccgac cataattgag ggcagggcac caggcaggat cacatggata
    13441 aacagcggta tgccggataa cccatagcta cgcgccatct ccaccagccc gcgatcgata
    13501 ttacggatgc catgccaggt gttgatataa atgggaaaca gcgtaccgag cgccaccaga
    13561 aagatttttg cggattcatc aatgccaaac cacaaaatca ccagcgggat cagcgccaga
    13621 tgcggcacgt tgcgcaacat ctgaattgag gtatccagca gccgttctcc ccagcgagat
    13681 agcccgctaa tcagtcccaa aatcaacccc agcgatccgc caattgaaaa gccaatcagc
    13741 gcccgctagg aactgatcgc cagatgctgc cacagttcgc cgctggcgga gagcgtccag
    13801 aacgccgtca ccaccccttc cggtgagggc aaaatacgcg tcgacagcca gccaactgag
    13861 gaggccagtt gccacaccgc cacgatgccc accggtaaaa actagggggc aacgcgcaat
    13921 aaccacttct tcactggcgt tgccattatt tcttccttag ctttgcgcga ctttacgggg
    13981 gataaaatca ttcgccaccg cttcgccttg cggattcagc ggctgcggct ggggaatttc
    14041 cggaatggcg acatccagat gtgggaacag caactcgccg acccgatacg cttcttccag
    14101 atgcggatag cccgaaagca caaagctgtc gatgccaagc gcggcgtatt cgttgattcg
    14161 cgcagcgacc gtaggaccat cgcccaccag cgccgttccg gcaccgccgc gcactaagcc
    14221 aacgcccgcc cataaattgg ggctgatctc cagattgtcg cgcttgccgt tatgtaacgc
    14281 cgccattcgc tgttgtccta cggaatccgt ccgggcgaat gcggcctgtg ctttggcgat
    14341 agtttcatca tcaagatgcg agattaaccg ctcggcggcc tgccacgctt cgtcgttagt
    14401 ttcacgaaca atcacatgca gacgaatacc gaaacgaatt ttgcgtccat gcgcggcagc
    14461 tttcgcccgc acttgttcga tcttctcttt tactagttct ggcggttcgc cccaggtgag
    14521 gtagagatca acttgttctg ccgccagctc ctgggcgacg tctgacgatc cgccaaagta
    14581 aagcggagga taaggctgtt gaatcggcgg gaagagcagt tttgctccgc gcacatgaat
    14641 atgtttaccg ttgaaattga cggtttcccc cagcaataag cgccgccaga cctaggtaaa
    14701 ttccgccgag gcttcgtagc gctcgctatg atcaaggaac acgccgtcgc ctgccagctc
    14761 ttgtggatcg ctgcctgtga ccagattaaa caacgcacga ccatttgaga gacggtcaag
    14821 cgtggcggcc tggcgagcgg caacggtagg tgaggttacg ctggggcgca gggcgacaag
    14881 aaacttcagc cgctgcgtca ccgggatcat cgacgccgcc accagccacg catcttcaca
    14941 cgatcgcccc gttgggatca gcacaccggt atagcccaga cgatccgccg cttgcgcaat
    15001 ttgttgcaga taaccgtgat caaccgggcg tgaaccttct tccgttccca gatagtgccc
    15061 gtcaccgtgg gtcggtaaaa accagaacat attcagactc ataattgttt tccttccagt
    15121 tgagtgggct gccagatgcg ctggcgaata tcgacttttt tcggcaccag acgattttca
    15181 taaaacagat ctgccgtttg ctgctgtaag gcggcaacct cggcgttaac cggtttgatg
    15241 gtggtaggag ggcgatgatc taagtaagag gcaatcaccg gtgccggtaa gcccatcgtt
    15301 tttgccagta aagcgatgct ttgctcgcgc tggctgcggg ttaacgcatc ggcctcacta
    15361 aaggttgcca gtacgccctg aacaaaagcg ccgttttttt ctgcataggg acgagctgcc
    15421 agataaaacg atccagtttg attgagatcg gtgccgtctt tcagcacccg catgccgccc
    15481 tgtaataatg cagcagagta gtagggatcc cagatagccc aggcgtcaac gttaccttgc
    15541 tggaacgcgg cgcgggcatc agcgggcgtc aggtaagtgg gttggatatc ggtaaactta
    15601 agtccggcct gacgcagtgc acgcagtaaa aggttgtgtg aactggaacc tttctgaaag
    15661 gcaactttgt gacctttaag atcggctacg gttttgatcg ggctgttttc tgccaccaga
    15721 atcacttcgg ctttgggctt cggtggctcg acgcccacat ataccaaatc agccccggca
    15781 gcctgggcaa agattggcgg aatatccccg gtactgccga gatcaatact gccaacgttt
    15841 aaggcttcca acatttgcgg acccgcgggg aactccaccc aggagatttt tgattgcgga
    15901 tagcgttttt ccagtaactg gtggcttttt gccagtacca taccaatact gcctttctga
    15961 tagcctatac ataacgcttc aggcgaggat tctgcagcaa ccgcaatcgt agagacgcta
    16021 agcaatccca ccagcgccag tttaatgatg ttacgcatgg gcattacctc gcagagacag
    16081 aaggtcagga acctgaacat cgcggcggtg caatgcctgc cagaaagttt ctagcgcggt
    16141 atcaagacgg gtttgcagat ttggcgtgaa ctggggtctg tgatggtaat caattacttg
    16201 tgagtcatcg gcaaacacgc cgtgcaggat ctcctgagct ttcagtgcgc ttaaaactgg
    16261 tttaagggca taatcgaccg ccagcagatg ggccacggta ccgcccgtcg ccagcggtag
    16321 caccactttg ccttgcaaag cgcgctctgg cagcaggtcg agcagggttt tcagcgcgcc
    16381 ggaataggcg gctttataca caggtgtagc gactatcagt ccatcagcct gttgcagctg
    16441 ttcggtgaag gtcttgagtg ccggactatc gaaacgagca taaagcagat cttccgggac
    16501 gaagttttgc agattccagt gataaacctc tacatccagg ccatttagtt tttcccgcgc
    16561 atattccagc aaggagctgg agcgagaagg aaagcgagga ctacccgcca gggtgatgac
    16621 acgcatactc tctccttata accaattgtt ctttttttgt taacattgat aacaattcgg
    16681 tcagtctgtc ggagagacaa gaaaatgcca aatataaatt ttgtgtatct ttttctgaaa
    16741 ataaactaaa gaaagggctt tatatatcac gcatatttat ttgttggata gtcattagat
    16801 gttgaatcaa tgaattactc gttcagttaa tatttaaata ttcatgaaat ctataaatta
    16861 aagatttgtc acttattgga tttagtattt ttgatttaaa tgacggtcaa ttattgttat
    16921 tattaagtct aatgtcattt aaaggcatta tcatatttac gaagagatca ggatgatgac
    16981 gatgaaaaaa agtgtattga cggcgtttat aactgtggta tgtgcaacgt ccagcgttat
    17041 ggcagctgat gataatgcta tcacggatgg caaagtaaca tttaatggta aagttattgc
    17101 tccagcttgt accctggtag ctgcgacgaa agattcagta gtcacattgc caaatgtcag
    17161 cgccaccaag ttgcaaacta atggagcagt ctctggcgtt aaaactgatg taccaattgc
    17221 cttagaaggc tgtgatgtta ctgtaactaa aaatgcgacg tttaccttta gtggtacggc
    17281 tgacggcgtg caacctacag catttgccaa ccaggctacc accgatgcag c
//
LOCUS       DADLFA010000083        17160 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000083 DADLFA010000000
VERSION     DADLFA010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17160
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.083"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            254..1432
                     /locus_tag="JQH65_002299"
     CDS             254..1432
                     /locus_tag="JQH65_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY6139692.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPLKFALLVVGRLILGFGESQLLTGALTWGLGIVGAKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(1429..2424)
                     /locus_tag="JQH65_002300"
     CDS             complement(1429..2424)
                     /locus_tag="JQH65_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HAY6139693.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            2523..3659
                     /gene="pdxB"
                     /locus_tag="JQH65_002301"
     CDS             2523..3659
                     /gene="pdxB"
                     /locus_tag="JQH65_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HAY6139694.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIAVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            3725..4738
                     /locus_tag="JQH65_002302"
     CDS             3725..4738
                     /locus_tag="JQH65_002302"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAY6139695.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            4738..5550
                     /gene="truA"
                     /locus_tag="JQH65_002303"
     CDS             4738..5550
                     /gene="truA"
                     /locus_tag="JQH65_002303"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAY6139696.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAPRKDAAWTLGVNANLPDDIAVRWVKA
                     VPDDFHARLSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            5633..6292
                     /locus_tag="JQH65_002304"
     CDS             5633..6292
                     /locus_tag="JQH65_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY6139697.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRCSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGMLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            6448..7362
                     /gene="accD"
                     /locus_tag="JQH65_002305"
     CDS             6448..7362
                     /gene="accD"
                     /locus_tag="JQH65_002305"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HAY6139698.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVEMGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPLGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            7432..8700
                     /gene="folC"
                     /locus_tag="JQH65_002306"
     CDS             7432..8700
                     /gene="folC"
                     /locus_tag="JQH65_002306"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAY6139699.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            8690..9352
                     /gene="dedD"
                     /locus_tag="JQH65_002307"
     CDS             8690..9352
                     /gene="dedD"
                     /locus_tag="JQH65_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HAY6139700.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            9611..10099
                     /gene="cvpA"
                     /locus_tag="JQH65_002308"
     CDS             9611..10099
                     /gene="cvpA"
                     /locus_tag="JQH65_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HAY6139701.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            10136..11653
                     /gene="purF"
                     /locus_tag="JQH65_002309"
     CDS             10136..11653
                     /gene="purF"
                     /locus_tag="JQH65_002309"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAY6139702.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQDYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            11748..12317
                     /locus_tag="JQH65_002310"
     CDS             11748..12317
                     /locus_tag="JQH65_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAY6139703.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            12583..13365
                     /gene="argT"
                     /locus_tag="JQH65_002311"
     CDS             12583..13365
                     /gene="argT"
                     /locus_tag="JQH65_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HAY6139704.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            13586..14368
                     /gene="hisJ"
                     /locus_tag="JQH65_002312"
     CDS             13586..14368
                     /gene="hisJ"
                     /locus_tag="JQH65_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HAY6139705.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            14458..15144
                     /locus_tag="JQH65_002313"
     CDS             14458..15144
                     /locus_tag="JQH65_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311219.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAY6139706.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFFGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            15141..15857
                     /locus_tag="JQH65_002314"
     CDS             15141..15857
                     /locus_tag="JQH65_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY6139707.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGIYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVLHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            15865..16638
                     /gene="hisP"
                     /locus_tag="JQH65_002315"
     CDS             15865..16638
                     /gene="hisP"
                     /locus_tag="JQH65_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HAY6139708.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEAHERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            16835..>17160
                     /locus_tag="JQH65_002316"
     CDS             16835..>17160
                     /locus_tag="JQH65_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HAY6139709.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDA"
BASE COUNT         3872 a         4192 c         4790 g         4306 t
ORIGIN      
        1 tcggataaga cgcgccagcg tcgcatccga cgttacgcgc caatgcggcc tccggcacta
       61 acgcaaaagg gaacctgatg gttccctttt tcacatcatt gacaatcgcc gccagttcca
      121 ggcaaacttc ccgctttgtc gatttccttc tgaaaagacg tacgcgttaa atcctgccaa
      181 cgcactgtaa ccctgaaacc agagagatga gacggggata ctcctcgcct tgcgctgcat
      241 tctggagtaa tgcatgactg ctgtaagcca aaccgaaaca cgatcttctg ccaatttttc
      301 gctcttccgc atcgcttttg cggtttttct cacctacatg accgtagggc tgccgttgcc
      361 ggttatcccg ctgtttgttc atcatgatct gggctatggc aataccatgg tcgggattgc
      421 cgtcgggatt cagtttctgg ctacggtgct gacgcgtggc tatgccgggc gactggccga
      481 tcaatatggt gcaaaacgtt cggcgcttca ggggatgtta gcttgtggtc tggctggcgg
      541 cgcgttgctg ctggcggcga ttttgcctgt ctccgcaccg ttgaaatttg ccctgttggt
      601 cgtcgggcgt ttgattcttg gctttggtga aagccagtta ctgacaggcg ctctgacctg
      661 gggattaggc atcgtagggg caaaacactc tggcaaagtg atgtcatgga acggaatggc
      721 gatttacggt gccctcgctg ttggtgcgcc gcttggcctg ttgattcata gccattacgg
      781 ttttgccgca ctggcgatca ccacaatggt attaccctta ctggcgtggg cctgtaacgg
      841 cacagtgcgc aaagtaccgg ccctggcggg agaacgtcca tcgctgtgga gcgttgtcgg
      901 gcttatctgg aaaccagggt tagggctggc actacaaggc gttggttttg cggttatcgg
      961 gactttcgtt tcgctctact ttgccagcaa aggatgggcg atggcgggct ttactcttac
     1021 cgcgtttggc ggcgcatttg tcgtgatgcg cgtcatgttt ggctggatgc cggaccgttt
     1081 tggcggcgtg aaagtggcga ttgtctctct gcttgtagaa acggtgggct tgttgctgct
     1141 ctggcaagcc ccaggtgcat gggtcgcatt agcgggcgcg gcgttaaccg gagccggatg
     1201 ttcgcttatc tttcctgcgc tgggcgtgga ggtggttaaa cgcgtcccct cacaagttcg
     1261 cggcaccgca ctgggcggtt acgccgcgtt tcaggatatc gccctcggcg tctccgggcc
     1321 gctggcggga atgctggcga ccacgtttgg ttactcttcg gtatttcttg ccggggcgat
     1381 ctctgcggtg ctgggtatta ttgtcacgat actgtcattt cgtcggggtt aacgaaccag
     1441 ccagaccagc atcaggataa tcgcgactaa cagtatccac agcgcgggac gcgttgccat
     1501 attttgcaac gtgtccatca tcggtggaaa aggattaagc agcggttgca tatcacgcgg
     1561 atcaatccct ttaacccgcc gttgataaag ctgattaaga atatcctcgg cctgggcaga
     1621 agaaagcgat gattgcgcag aaaggccata tttttgctgg atatatgccg ataacgccgc
     1681 cagttcactg gcatctaaag gttgttttag cgccatctgt agtgattcca gcgtcggcgt
     1741 attttgctgg cttagcgtct gacgcgcctg tagccaggtc accagatggt taaacagttt
     1801 cgctggaatt aactcgccat ctttcacccc ggaaagttcc agcatcgatt gccagatctg
     1861 tttgctgggt tcccccgttg ccgccgcaag tttggtcacc agctgtttta gcgcattgtg
     1921 ctccgccggt aataaaggac ggtcggtcgc ctcgcgctgc tgcggttgcg gaataaccat
     1981 cttcccttcc tgcaacaggg tgagaatggt ttttaattgc tccggtgaga gctgattcag
     2041 cggcgtctga ccaaagttat gacggatata atccgtgacc gcctgacgat tattccccag
     2101 acgtaaatac tcccctaatt gcgctaaaag ctggcgggca gaatggcttt tttgcgcggc
     2161 cagcagacgt tgcgccagat tatgctcagc ggcagggaag tgacgcgaaa gcagcggtga
     2221 atctcccgac agaccaatat cgtgcctgat acccgcccac agttctgctc tttgctgttg
     2281 cgtcagtgag gtcactttcg tcattaagct ttccagcgaa gtacgttgct gactggataa
     2341 aggctgattg cccgcgccag acggcaggtt atctccctga cctggtggtt gcccaggagg
     2401 agggccggaa ataggttgta tcattacgta tccttatacc tgaaatcttc gcaagtatgc
     2461 ctggccgcga gattatggca cacttgtccg gttaactctc gtctcataca ggtaacacaa
     2521 acgtgaaaat ccttgttgat gaaaatatgc cttatgcccg cgacttattt agccgtttgg
     2581 gtgaggtgac cgcggttccc gggcgtccaa tcgccgtcgc tcaactggca gacgcggatg
     2641 cgctgatggt gcgttcggtc acgaaagtga atgaatcttt gctggcagga aaacccatta
     2701 aatttgttgg cactgccact gcggggaccg accatgtcga tgaagcatgg ttgaagcagg
     2761 cgggaattgg tttttccgct gcacctggct gtaatgcgat tgcggtggtg gaatatgttt
     2821 tctcctcact actgatgctt gccgaacgcg atggattttc actgcacgac cgtacggtgg
     2881 ggatcgtggg cgttggtaac gttggacgtc gattgcaggc gcgactggaa gcgttaggga
     2941 ttaaaacatt gctttgcgat ccgcctcgcg ctgaccgtgg ggatgagggt gatttccgct
     3001 cgctggatga attagttcag catgcggata ttctgacttt ccatacgcca ctcttcaaag
     3061 atgggccgta caaaacgctg catctggcgg atgaaaaact gatccgtagc ctgaagccgg
     3121 gagcgattct gattaacgcc tgccgtggcg cagtcgtcga taatactgcg ttgctgacct
     3181 gcctgaatga aggccagaag ttaagcgtag tgctggatgt ctgggaaggc gaaccggaac
     3241 ttaacgtgga gttgctgaca aaagtggata tcggcacgcc gcatatcgca ggttataccc
     3301 tggaaggtaa agcacgcggt actacgcaag tgtttgaagc ttatagcaag tttattgggc
     3361 atgaacagca cgttgcgctg gatacattac tgcccgcgcc agagtttggt cgcattacgc
     3421 tgcatggccc gctcgatcaa ccgacgctga aaaggctggt gcatttggtg tatgatgtgc
     3481 gccgcgatga cgcaccgctg cgtaaagtcg ccgggatacc gggtgagttc gataaactgc
     3541 gcaaaaacta tcttgagcgc cgtgaatggt catctctgta tgtaatttgt gatgacgcca
     3601 gtgcggcatc attgctgtgt aaactgggtt ttaacgccgt tcatcatccg gcacgttaat
     3661 ctcttcttca tgctctctgc tgtaacattg gcagggagct ttgctatttc tggagtaaac
     3721 caccatgtct gaaggctgga acattgccgt cctgggcgca actggcgctg tgggcgaagc
     3781 cctgcttgaa acgctggctg aacgtcagtt cccggttggg gaaatttatg cactggcacg
     3841 taacgaaagc gcaggcgaac aactgcgctt tggtggtaaa acaatcaccg tgcaggatgc
     3901 cgctgaattc gactggacgc aggcgcagct ggcatttttt gtcgcaggca aagaagctac
     3961 cgctgcctgg gttgaagaag cgaccaactc aggttgcctg gtgatcgaca gcagcggact
     4021 gtttgctctc gaacccgacg taccgctggt ggtgccggaa gtaaacccgt ttgtactgac
     4081 cgattaccgc aaccggaatg tcatcgccgt accagacagt ctgaccagcc agctgctggc
     4141 ggcactgaaa ccgttaatcg atcagggcgg tttgtcgcgt atcagtgtta ccagcctgat
     4201 ttcagcctcc gcccagggca aaaaagcggt cgatgcgtta gcggggcaga gtgcgaaatt
     4261 gctcaacggc attccgattg acgaagaaga tttcttcggt cgtcaactgg cgttcaatat
     4321 gctgccgttg ttgccggata gcgaaggtag cgtgcgtgaa gaacgtcgta tcgttgacga
     4381 agtacgcaaa atcctgcagg acgaagggct gatgatttcg gcaagcgtcg tccaggcacc
     4441 ggtattctac ggtcatgccc agatggtcaa ctttgaagca ctgcgtccgc tggcggcaga
     4501 agaagcgcgt gatgcgtttg ctcagggcga agatattgtg ctctctgaag agaacgaatt
     4561 cccgactcag gtaggggatg cttcgggtac tccgcatctt tctgttggct gcgtgcgtaa
     4621 tgactacggt atgccggagc aagtccagtt ctggtcggtg gccgataacg ttcgctttgg
     4681 cggcgcgctg atggcagtaa aaatcgccga gaaactggtg caggagtatc tgtactaatg
     4741 tccgaccagc aacaactgcc agtttataaa attgcgctgg gcattgagta cgacggcagt
     4801 aagtattacg gctggcaacg gcagaatgaa gtccgcagtg tgcaggagaa gctggaaaag
     4861 gcgctctccc aggtggcgaa cgaacccatc accgtcttct gcgccgggcg tactgacgca
     4921 ggggtacacg gtaccgggca ggtggtgcat ttcgaaacaa ccgcgccgcg caaagacgcg
     4981 gcgtggacct tgggcgtaaa tgcgaattta cctgatgaca tcgccgtgcg ttgggttaaa
     5041 gctgtacctg atgattttca tgcccgactt agcgccacgg ctcgccgtta tcgctacatc
     5101 atctacaatc atcggctgcg cccggcggta ctgagtaaag gggtaaccca tttttacgaa
     5161 ccgctggacg ctgaacggat gcatcgggct gcgcaatgct tgctgggcga gaatgatttc
     5221 acctcgttcc gtgcggtgca gtgccagtcc cgaaccccgt ggcgcaacgt tatgcatatt
     5281 aatgtcacgc gtcacggtcc ttatgtggtg gtagatatca aagcgaatgc ctttgtacat
     5341 catatggtca ggaatattgt cggcagcctg atggaagtcg gtgcccacaa ccagccggag
     5401 agctggatag cagagttgct ggcggcaaag gacagaacgc tggcggcagc aacggcaaaa
     5461 gcggaagggc tgtatctggt cgcggtggat taccctgacc ggtatgatct tccaaaaccg
     5521 ccaatgggcc cgctatttct ggcggactaa cgagaataat gctcggaaca tttcaggctg
     5581 tagccaacgt agttacagcc tgaaagatga cgagtacaag gcataggcaa atatggacct
     5641 gatttatttc ctcatcgatt ttatcctgca cattgacgtg catctggcag agctggtcgc
     5701 ggagtacggc gtctgggttt atgccattct gtttttgatt ctgttctgcg aaacggggct
     5761 ggtggtaacg ccattcctgc ctggtgattc gctgctgttt gttgctggtg cattggcgtc
     5821 gctggaaact aacgatctca atgtccatat gatggtggtg cttatgttga ttgccgctat
     5881 tgtcggtgat gcagtgaact ataccattgg acggttattt ggtgacaaac tgttcagtaa
     5941 cccggattcg aaaatctttc gttgcagtta tcttgataag actcatcagt tttacgagag
     6001 acatggcggc aaaacgatta ttctcgcccg ttttgtaccg atcgtcagaa cgttcgcgcc
     6061 gtttgttgcg ggaatgggcc acatgtcgta ccgtcatttc gccgcttata acgtgatcgg
     6121 cgcactgttg tgggtactga tttttaccta cgcgggttat ttcttcggca tgctcccctt
     6181 cattcagagc aaccttaagc tgatgattgt tggcattatt tttgtttcaa tattgcctgg
     6241 ggttttcgaa tttattcgtc acaaacgcgc tgcggcacgc gccgcaaaat aaaaagtaac
     6301 tccgcggttc gaccactttt ttatccaaag tttcgggctg ttatgtttta atgtgcaaca
     6361 ttcatggtct gttgggggca aaaatggcat tatgcgtccc caaagataaa actggcatcg
     6421 aaccaggttc agacagaaag gtccctaatg agctggattg aacgaattaa aagcaacatt
     6481 actcccaccc gcaaggcgag cattcctgaa ggggtgtgga ctaagtgtga tagctgcggt
     6541 caggttttat accgcgctga gctggaacgt aatcttgagg tctgtccgaa gtgtgaccat
     6601 cacatgcgta tgacagcgcg taatcgcctg catagcctgt tagatgaagg aagccttgtg
     6661 gagatgggta gcgagcttga gccgaaagat gtgctgaagt ttcgtgactc caagaagtat
     6721 aaagaccgtc tggcttctgc gcagaaagaa accggcgaaa aagatgcgct ggtggtgatg
     6781 aaaggcactc tgtatggaat gccggttgtc gctgcggcat tcgagttcgc ctttatgggc
     6841 ggttcaatgg ggtctgttgt gggtgcacgt ttcgtgcgtg ccgttgagca ggcgctggaa
     6901 gataactgcc cgctgatctg cttctccgcc tctggtggcg cacgtatgca ggaagcactg
     6961 atgtcgctga tgcagatggc gaaaacctct gcggcactgg caaaaatgca ggagcgcggc
     7021 ttgccgtaca tctccgtgct gaccgacccg acgatgggcg gtgtttctgc aagtttcgcc
     7081 atgctgggcg atctcaacat cgctgaaccg aaagcgttaa ttggctttgc cggtccgcgt
     7141 gttatcgaac agaccgttcg cgaaaaactg ccgctaggat tccagcgcag tgaattcctg
     7201 atcgagaaag gcgcgatcga catgatcgtc cgtcgtccgg aaatgcgcct gaaactggcg
     7261 agcattctgg cgaagttgat gaatctgcca gcgccgaatc ctgaagcgcc gcgtgaaggc
     7321 gtagtggtac ccccggtacc ggatcaggaa cctgaggcct gataactgat aagggcaggg
     7381 ccactggctc tgcccttttg ctattctcac cgtaacgaat cagcggatac catgattatc
     7441 aaacgcactc ctcaagccgc gtcgcctctg gcttcgtggc tttcttatct ggaaaacctg
     7501 cacagtaaaa ctatcgatct cggccttgag cgcgtgagcc aggtcgcggc tcgtcttggc
     7561 gttctgaaac cagcgccatt tgtgtttacc gttgcgggta cgaatggcaa aggcaccacc
     7621 tgccgtacgc tggagtcgat tctgatggcg gcagggtaca aagtgggcgt ctacagttcg
     7681 ccgcatctgg tgcgttatac cgagcgcgta cgtgtgcagg gtcaggaatt gccggaatcg
     7741 gcccacaccg cctcttttgc ggagattgaa tcggcacgcg gtgatatttc cctgacctat
     7801 ttcgagtacg gtacgctgtc ggcgttgtgg ctgttcaaac aggcacaact tgacgtggtg
     7861 attctggaag tagggctggg cggtcgtctg gacgcaacca atattgtcga tgccgatgtc
     7921 gcggtagtaa ccagtattgc gctggatcat accgactggc tggggccaga tcgcgaaagt
     7981 attggtcgcg agaaagcagg catctttcgc agcgcaaaac cggcaattgt cggtgagccg
     8041 gaaatgcctt ctaccattgc tgatgtggcg caggaaaaag gtgcactgtt acaacgtcgg
     8101 ggcgttgagt ggaactattc cgtcaccgat catgactggg cgtttagcga tgctcacggc
     8161 acgctggaaa atctgccgtt gccgcttgtc ccgcaaccga atgccgcaac agcgctggcg
     8221 gcactgcgtg ccagcgggct ggaagtcagt gaaaatgcca ttcgcgacgg gattgccagc
     8281 gcaattttgc cgggacgttt ccagattgtg agcgagtcgc cacgcgttat ttttgatgtc
     8341 gcgcataatc cacatgcggc ggaatatctc accgggcgta tgaaagcgct accgaaaaac
     8401 gggcgcgtgc tggcggttat cggtatgcta catgataaag atattgccgg aactctggcc
     8461 tggttgaaaa gcgtggttga tgactggtat tgtgcgccac tggaagggcc gcgcggtgcc
     8521 acggcagaac aactgcttga gcatttgggt aacggcaaat catttgatag cgttgcgcag
     8581 gcatgggatg ccgcaatggc ggacgctaaa gcggaagaca ccgtgctggt gtgtggttct
     8641 ttccacacgg tcgcacatgt catggaagtg attgacgcga ggagaagcgg tggcaagtaa
     8701 gtttcagaat cggttagtgg gcacgatcgt gctggtggcg ctgggggtga ttgtacttcc
     8761 agggctgctg gacgggcaga aaaaacatta tcaggatgag tttgcggcta tcccgctggt
     8821 tccaaaagcg ggcgatcgcg atgagcctga tatgatgcca gccgccaccc aggcgttgcc
     8881 gacgcagccg ccggaaggcg cagcggaaga ggtgcgggca ggtgatgccg cagcaccgtc
     8941 gctcgatccg gccactattg cagccaataa caccgagttt gaaccggaac ctgcaccggt
     9001 cgccccaccg aagccgaaac cggtggagcc gcctaaaccg aaggttgaag caccacctgc
     9061 gccgaagcca gaaccgaagc cggtcgtgga agaaaaagca gcgccaacgg gtaaagccta
     9121 tgttgtgcaa ctgggtgcgc tgaaaaatgc cgataaagtg aatgagattg tcggtaagct
     9181 gcgcggtgcc ggttatcggg tttatacgtc gccatccacg ccagtgcagg gtaaaattac
     9241 ccgtattctg gttgggccgg atgcctcgaa agataagctg aaaggttcgc tgggtgagtt
     9301 gaagcaactt tctggcttaa gtggcgtggt aatgggctat acgccgaatt aatacagtct
     9361 tgcctgatgc gacgctggcg cgtcttatca ggcttgggca gtgttagaac cgtaggtcgg
     9421 ataaggcgtt tacgccgcat ccgacacgca ttgcacgatg ccgcaaaggc ataaaaagtc
     9481 gatggcgttg aatatttttt cagcgccatt tttattgatg cgtgggaagg aaatccctac
     9541 gcaaacgttt tctttttctg ttagaatgcg ccccgaacag gatgacaggg cgtaaaatcg
     9601 tgggacacat atggtctgga ttgattacgc cataatcgcg gtgattgctt tttcctctct
     9661 ggttagcctg atccgcggct ttgttcgtga agcgttatcg ctggtgacat ggggttgtgc
     9721 tttctttgtt gccagtcatt actacactta cctgtcagtc tggtttacgg gctttgaaga
     9781 cgaactggtt cgaaatggaa ttgccatcgc ggtactgttt atcgctaccc tgatcgttgg
     9841 tgctatcgtg aacttcgtga taggccagtt ggtggagaaa acggggttgt caggcaccga
     9901 tcgggtgctg ggcgtctgtt tcggtgcgtt gcgcggtgtg ttgattgttg ccgccattct
     9961 cttctttctc gactccttta ccggggtgtc gaaaagcgaa gactggagca aatcacagct
    10021 gatcccgcag ttcagtttta tcatcagatg gttttttgat tatctgcaaa gctcgtcaag
    10081 tttcttgccc agagcgtaag tgctctgaga tgtggcttaa cgaggaaaaa gacgtatgtg
    10141 cggtattgtc ggtatcgccg gtgttatgcc ggttaaccag tcgatttatg atgccttaac
    10201 ggtgcttcag catcgcggtc aggatgccgc cggcatcatc accatagatg ccaataactg
    10261 cttccgtttg cgtaaagcga acgggctggt gagcgatgta tttgaagctc gccatatgca
    10321 gcgtttgcag ggcaatatgg gcattggtca tgtgcgttac cctacggctg gcagctccag
    10381 cgcctctgaa gcgcagccgt tttacgttaa ctccccgtat ggcattacgc ttgcccacaa
    10441 cggcaatctg accaacgctc acgagttgcg taaaaaactg tttgaagaaa aacgccgcca
    10501 catcaacacc acttccgact cggaaattct gcttaatatc ttcgctagcg agctggacaa
    10561 cttccgccac tacccgctgg aagccgacaa tattttcgct gccattgctg ccacaaaccg
    10621 cttaatccgc ggcgcatatg cctgtgtggc gatgattatc ggccacggta tggttgcttt
    10681 ccgcgatcca aacgggattc gtccgctggt actgggaaaa cgtgatattg acgagaaccg
    10741 tacagaatat atggtcgctt ccgaaagcgt agcgctcgat acgctgggct ttgatttcct
    10801 gcgtgacgtc gcgccgggcg aagcgattta catcactgaa gaagggcagt tgtttacccg
    10861 tcaatgtgct gacaatccgg tcagcaatcc gtgcctgttt gagtatgtat actttgcccg
    10921 cccggactcg tttatcgaca aaatttccgt ttacagcgcg cgtgtgaata tgggcacgaa
    10981 actgggtgag aaaattgccc gcgaatggga agatctggat atcgacgtgg tgatcccgat
    11041 cccggaaacc tcgtgtgata tcgcgctgga aattgctcgt attctgggca agccgtaccg
    11101 ccagggcttc gttaaaaacc gctatgttgg ccgcaccttc atcatgccgg gccagcagct
    11161 gcgtcgtaag tccgtgcgcc gtaaactgaa cgccaaccgc gccgagttcc gcgataaaaa
    11221 cgtcctgctg gtcgacgact ccatcgtccg tggcaccact tctgagcaga ttatcgagat
    11281 ggcacgcgaa gccggagcga agaaagtgta cctcgcttct gcggccccgg aaattcgctt
    11341 cccgaacgtt tatggtattg atatgccgag cgccacggaa ctgatcgctc acggtcgcga
    11401 agttgatgaa attcgccaga tcatcggtgc tgacgggttg attttccagg atctgaacga
    11461 tctgatcgac gccgttcgcg ctgaaaatcc ggatatccag cagtttgaat gctcggtgtt
    11521 caacggcgtc tacgtcacca aagatgttga tcaggactac ctcgatttcc tcgatacgtt
    11581 acgtaatgat gacgccaaag cagtgcaacg tcagaacgaa gtggaaaatc tcgaaatgca
    11641 taacgaagga tgatgccttc gctgagggtg ccggtctggc accctgactt gcaactcccg
    11701 ccgaaatact gcaaagtctg cctgcaagtc tgacagggca actatttatg aaacgactca
    11761 ttgtaggcat cagcggtgcc agcggcgcga tttatggcgt gcgcttatta caggttctgc
    11821 gcgatgtcac agatatcgaa acgcatctgg tgatgagcca ggcggcgcgc cagaccttat
    11881 ccctcgaaac ggatttttcc ctgcgcgaag tgcaggcatt agctgatgtc acgcacgatg
    11941 cgcgcgatat ttccgccagc atctcttccg gttctttcca gacgctgggg atggtgattt
    12001 taccctgttc aatcaaaacc ctttccggca ttgtccatag ctatactgat ggcttactga
    12061 cccgtgcggc agatgtggtg ctgaaagagc gtcgcccgtt ggtgctctgc gtgcgtgaaa
    12121 caccattgca cttaggccat ctgcgtttaa tgactcaggc ggcagaaatc ggtgcggtga
    12181 ttatgcctcc tgttccggcg ttttatcatc gcccgcaatc ccttgatgat gtgataaatc
    12241 agacggttaa tcgtgttctt gaccagtttg caataaccct tcctgaagat ctctttgccc
    12301 gctggcaggg cgcataactc cgctgttgcc ctgtttcagg gcaattttgc aaccgcgatc
    12361 aaatcctcga cattttgttt ctgccattca atcgaaacgc tgcgattcaa ccgctatacc
    12421 tgctatcttc aacttcagga caataatgca acgtcttatt aacatattta acgttgaatg
    12481 ttactgttgt cgtcaagatg gcataagacc tgcttgaaag agcctgcaaa cacacaacac
    12541 aatacacaac ataaaaaagc cattttcact tgagggttat gtatgaagaa gtcgattctc
    12601 gctctgtctt tgttagtcgg tctctccgca gcggcttcca gctatgcggc gctaccggag
    12661 acggtacgta tcggaaccga taccacctat gcgccgttct cgtcgaaaga tgctaaaggt
    12721 gattttgttg gctttgatat cgatctcggt aacgagatgt gcaaacggat gcaggtgaaa
    12781 tgtacctggg ttgccagtga ctttgacgcg ctgatcccct cactgaaagc gaaaaaaatc
    12841 gacgccatta tttcgtcgct ttccattacc gataaacgtc agcaggagat tgccttctcc
    12901 gacaagctgt acgccgcaga ttctcgtttg attgctgcca aaggttcacc gattcagcca
    12961 acgctggatt cactgaaagg taaacatgtt ggtgtgctgc agggatcaac ccaggaagct
    13021 tacgctaacg agacctggcg tagcaaaggc gtggatgtgg tggcctatgc caaccaggat
    13081 ttggtctatt ccgatctggc tgcaggacgt ctggatgctg cgttacaaga tgaagttgct
    13141 gccagcgaag gattcctcaa gcaacctgct ggtaaagatt tcgcctttgc tggctcatca
    13201 gtaaaagaca aaaaatactt cggtgatggc accggtgtag ggctacgtaa agatgatgct
    13261 gaactgacgg ctgccttcaa taaggcgctt ggcgagctgc gtcaggacgg cacctacgac
    13321 aagatggcga aaaagtattt cgactttaat gtctacggtg actgatacgt cgctgggaag
    13381 ctgtacctga tggaatgatc atcatggtgc acgctaggtt tgttgcacta tcgtggtgca
    13441 ttgaaatgca tacttaagca tttttaatga aaaataatac atctaacggg gcgggatatt
    13501 ttgccttgat ggtcaatttt atggcacgat aagtgtaaca aacctgtaaa tattccctat
    13561 aaaaagactg tcagttgagg acattatgaa aaaactggtg ctatcgctct ctctggttct
    13621 ggccttctcc agcgcaactg cggcatttgc tgcgattccg caaaacatcc gcatcggtac
    13681 cgacccgacc tatgcgccat ttgaatcaaa aaattcacaa ggcgaactgg ttggcttcga
    13741 tatcgatctg gcaaaggaat tatgcaaacg catcaatacg caatgtacgt ttgtcgaaaa
    13801 tccgctggat gcgttaatcc cgtccttaaa agcgaagaag attgacgcca tcatgtcatc
    13861 gctttccatt acggaaaaac gtcagcaaga aatagccttc accgacaaac tgtacgctgc
    13921 cgattctcgt ttggtggtgg cgaaaaattc tgacattcag ccgacagtcg agtcgctgaa
    13981 aggcaaacgg gtaggcgtat tgcagggcac cacccaggag acgttcggta atgaacattg
    14041 ggcaccaaaa ggcattgaaa tcgtctcgta tcaggggcag gacaacattt attctgacct
    14101 gactgccgga cgtattgatg ccgcgttcca ggatgaggtc gctgccagcg aaggtttcct
    14161 caaacaacct gtcggtaaag attacaaatt cggtggcccg tctgttaaag atgaaaaact
    14221 gtttggcgta gggaccggca tgggcctgcg taaagaagat aacgaactgc gcgaagcact
    14281 gaacaaagcc tttgccgaaa tgcgcgctga cggtacttac gagaaattag cgaaaaagta
    14341 cttcgatttt gatgtttatg gtggctaatc gccatcggtg aaagaaagcc cgctcccttc
    14401 gggtaaccgg agggagacaa gaggcggtaa ttcaccacac acgacaggac aggcagcatg
    14461 ttatatgggt tttcaggtgt tattttacag ggtgcgctcg tcacgctgga gctggctatc
    14521 agctctgtag tgctcgctgt aatcatcggt ttaattggcg ctggcggtaa gctctcgcaa
    14581 aatcggcttt cggggctgat tttcgaaggg tacaccacgc tgattcgtgg cgtgccggac
    14641 ttagtgttga tgctgctgat tttcttcggt ttgcagattg cgctaaacac ggtgacggag
    14701 gcgatgggcg tcgggcagat tgatatcgat ccgatggtcg ctggtattat cactctcggt
    14761 tttatctacg gtgcttattt taccgaaacg ttccgtggtg cttttatggc agtgccgaaa
    14821 ggacatatag aggcggcgac ggcgttcggt tttactcgtg ggcaagtgtt tcggcggatc
    14881 atgtttccgg cgatgatgcg ttacgccctg ccaggcattg gcaacaactg gcaggtgatc
    14941 ctcaaatcta ccgcactggt ttcgttactc ggcctggaag atgtggtcaa agccacccaa
    15001 ctggcaggca aaagtacctg ggaaccgttc tatttcgcca tcgtctgtgg cgtgatttac
    15061 ctggttttca ccactgtttc caatggtgtg ctgctgttcc ttgagcgccg ctactccgtg
    15121 ggtgtgaaga gggctgacct gtgatcgaaa tcttacatga atactggaaa ccgctgctgt
    15181 ggaccgacgg ttatcgcttt actggtgtgg cgatcactct gtggctgctt attttgtcgg
    15241 tagtgatagg cggagtcctg gcgctgtttc tggcgattgg tcgtgtctcc agtaataaat
    15301 acatccagtt tccaatctgg ttatttacct atatttttcg cggtacgccg ctgtatgttc
    15361 agttgctggt gttctattcc ggcatataca cgcttgagat tgttaaggga accgaattcc
    15421 ttaacgcttt cttccgcagt ggcctgaact gtaccgtgct ggcgctgacg cttaacacct
    15481 gcgcttacac taccgagatt tttgctgggg caatccgttc ggttctgcat ggggaaattg
    15541 aagccgccag agcctatggc ttctcgactt ttaaaatgta tcgttgcatt attttgcctt
    15601 ctgcgctgcg tattgcgtta ccggcataca gcaacgaagt gatcctgatg ctgcactcta
    15661 ctgcgctggc gtttacggcc actgtgccgg atctgctgaa aatagcccgc gatattaacg
    15721 ccgccacgta tcaacctttt accgccttcg gcattgccgc ggtgctctat ttaatcatct
    15781 cttatgtcct gatcagcctc tttcgcagag cggaaaaacg ctggttgcag catgtgaaac
    15841 cttcttcaac gcactgagaa tacgatgtcc gagaataaat taaacgttat cgatttgcac
    15901 aaacgctacg gcgaacatga agtgctgaaa ggggtctcac tgcaagcgaa tgccggagat
    15961 gtaataagca tcatcggatc gtcgggatcg gggaaaagta cctttctgcg ctgcattaac
    16021 ttcctcgaaa aaccgagtga agggtcgatc gtggtcaatg gccagacgat caatctggtg
    16081 cgcgacaaag acggtcaact caaagtcgcc gataaaaatc aactgcgctt actgcgcaca
    16141 cgcctgacga tggtattcca gcacttcaat ctctggagcc atatgacggt gctggaaaac
    16201 gtcatggaag cgccgattca ggtgttgggc ctgagcaagc aggaagcgca cgagcgggcg
    16261 gtcaagtatc tggcaaaagt cgggatagac gaacgtgcgc aggggaaata tccggtgcat
    16321 ctttccggcg gtcagcaaca gcgtgtttct atcgcgcggg cgctggcgat ggaaccggaa
    16381 gttttactgt ttgatgaacc tacctcggcg ctcgatcctg aactggtagg cgaagtgttg
    16441 cgtattatgc agcaactggc agaagagggg aaaaccatgg tggtagtgac tcacgaaatg
    16501 ggctttgctc gccatgtttc tactcatgtc attttcctcc atcaggggaa aatagaagaa
    16561 gagggcgcgc cggagcagtt atttggcaac ccgcaaagcc ctcgtctgca acagttcctt
    16621 aagggatcgc tgaaataatt tcctctccgg tggctaacgc tgccggagta ttttcatggg
    16681 gaatttgcga gtcagcttcc gctgaaatgt aaaagttgca gtccgctaaa aatacttctg
    16741 aattcgctgt ccaaagataa aaactgcctg ctgcattggg tgttaatcgt ggttattgtc
    16801 gcgtcatctt aaatttgagg ggtgacggaa cgccatgaca atatcgacaa cttccacgcc
    16861 gcatgatgcg gtatttaaat cttttttacg ccatccagac accgcgcggg attttattga
    16921 tattcatctt cccgcgccgc tgcgcaaact gtgtgattta acgacgctta aactggaacc
    16981 aaacagtttt attgatgaag acctgcggca atattattcc gacctcttgt ggtctgtgaa
    17041 aacgcaggag ggagtgggtt atatttatgt agtgatagag caccaaagta agccggaaga
    17101 attaatggct tttcgcatga tgcgttattc cattgcggca atgcaaaacc atcttgatgc
//
LOCUS       DADLFA010000084        17104 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000084 DADLFA010000000
VERSION     DADLFA010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17104
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.084"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..303)
                     /gene="hlyE"
                     /locus_tag="JQH65_002317"
     CDS             complement(<1..303)
                     /gene="hlyE"
                     /locus_tag="JQH65_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HAY6139710.1"
                     /translation="MTEIVADKMVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETI
                     KELSRFKQEYSQAASVLVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLF"
     gene            676..1095
                     /gene="umuD"
                     /locus_tag="JQH65_002318"
     CDS             676..1095
                     /gene="umuD"
                     /locus_tag="JQH65_002318"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HAY6139711.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            1095..2362
                     /locus_tag="JQH65_002319"
                     /pseudo
     CDS             1095..2362
                     /locus_tag="JQH65_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
     gene            complement(2408..2938)
                     /gene="dsbB"
                     /locus_tag="JQH65_002320"
     CDS             complement(2408..2938)
                     /gene="dsbB"
                     /locus_tag="JQH65_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HAY6139712.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     MISQPFKAKKRDLFGR"
     gene            complement(3084..4625)
                     /gene="nhaB"
                     /locus_tag="JQH65_002321"
     CDS             complement(3084..4625)
                     /gene="nhaB"
                     /locus_tag="JQH65_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HAY6139713.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            4847..5566
                     /gene="fadR"
                     /locus_tag="JQH65_002322"
     CDS             4847..5566
                     /gene="fadR"
                     /locus_tag="JQH65_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HAY6139714.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(5618..7150)
                     /locus_tag="JQH65_002323"
     CDS             complement(5618..7150)
                     /locus_tag="JQH65_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HAY6139715.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            7480..8778
                     /locus_tag="JQH65_002324"
     CDS             7480..8778
                     /locus_tag="JQH65_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HAY6139716.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            8846..9847
                     /gene="dadX"
                     /locus_tag="JQH65_002325"
     CDS             8846..9847
                     /gene="dadX"
                     /locus_tag="JQH65_002325"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005005651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HAY6139717.1"
                     /translation="MSARPRRTRVVKANAYGHGIERIWSALGATDGFALLNLEEAITL
                     RERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHSNWQLKALQNARLKAPLDIYLKVN
                     SGMNRLGFQPDRVLTVWQQLRAMANVGEMILMSHFAEAEHPDGISSAMARIEQAAEGL
                     ECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPSGQWRDIANTGLRPVMTLSSEIIG
                     VQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGYPRHAPTGTPVLVDGVRTMTVGTV
                     SMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAAAAGTVGYELMCALALRVPVVTV"
     gene            complement(10234..11970)
                     /locus_tag="JQH65_002326"
     CDS             complement(10234..11970)
                     /locus_tag="JQH65_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HAY6139718.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGTAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(12065..12979)
                     /gene="ldcA"
                     /locus_tag="JQH65_002327"
     CDS             complement(12065..12979)
                     /gene="ldcA"
                     /locus_tag="JQH65_002327"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HAY6139719.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNKTFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVSAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            13079..13690
                     /gene="emtA"
                     /locus_tag="JQH65_002328"
     CDS             13079..13690
                     /gene="emtA"
                     /locus_tag="JQH65_002328"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HAY6139720.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(13692..14426)
                     /locus_tag="JQH65_002329"
     CDS             complement(13692..14426)
                     /locus_tag="JQH65_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HAY6139721.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            14627..14881
                     /locus_tag="JQH65_002330"
     CDS             14627..14881
                     /locus_tag="JQH65_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAY6139722.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFQTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGLFRLLRRE"
     gene            15060..15500
                     /locus_tag="JQH65_002331"
     CDS             15060..15500
                     /locus_tag="JQH65_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6139723.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     RQRRCKSPGDDSETLTRRNDAIFGNHVWQTFAQYFPPGLEKPAV"
     gene            complement(15579..16924)
                     /gene="treA"
                     /locus_tag="JQH65_002332"
                     /pseudo
     CDS             complement(15579..16924)
                     /gene="treA"
                     /locus_tag="JQH65_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreA"
BASE COUNT         4309 a         4223 c         4423 g         4149 t
ORIGIN      
        1 aaatagcaaa atatacgctg cgagcaattg cgtcgcaaca ccacaccatt catacactgt
       61 ttgggttgct tcaaaatact tatcctggct atccataagt aaggttttaa tatcgccgac
      121 taaaacggag gctgcctgtg aatactcctg tttaaagcga cttaactctt ttatggtttc
      181 atcaaaggtc tgccagggga tgacctgatc gagatattta ttataaagat ctaatgctcc
      241 atctgcggtt tcgattgcgt ttttaactac ttctaccatt ttatctgcaa cgatttcagt
      301 cataatcatt cgcctcttta aatatataaa ttgtaatgaa actcctgttt tacaactatt
      361 aataaatttt acttcatcta attcatagtt agccgggtgg gatgcgtcaa tgtctttatt
      421 tctattaata tgataaatat caaacaatgt ttaatgtcat tatggcgaat gcttctattc
      481 tatttttttg ccgggtgata tttttcattt ctgctggatg agcgtcgtcg ccagaaggcc
      541 acgtgagcac aggataagag aacgaaaaat cagcagtcta tacagcgaca aatattgata
      601 gcctgaatca gtattgatct gctggcaaga atagactact gtatataaaa acagtataac
      661 ttcaggcaga ttattatgtt gtttatcaag cctgcggatc tccgcgaaat tgtgactttt
      721 ccgctattta gcgatcttgt tcagtgtggc tttccttcac cggcagcaga ttacgtcgaa
      781 cagcgcatcg atctgaatca actgttgatc cagcatccca gcgcgactta cttcgtcaaa
      841 gcaagtggtg attctatgat tgatggtgga attagtgacg gtgatttact gattgtcgat
      901 agcgctatta ccgccagcca tggtgatatt gtcatcgctg ctgttgacgg cgagtttacg
      961 gtgaaaaaat tgcaactacg cccgacggta cagcttattc ccatgaacag tgcgtactcg
     1021 cccattacca tcagtagcga agatacgctg gatgtctttg gtgtggtgat ccacgtcgtt
     1081 aaggcgatgc gctgatgttt gctctctgtg atgtaaacgc gttttatgcc agctgtgaga
     1141 cggtgtttcg ccctgattta tggggtaaac cggtggttgt actatcgaat aatgacggtt
     1201 gcgttatcgc ccgaaacgct gaggcaaagg cgctgggtgt taaaatgggc gatccctggt
     1261 tcaaacaaaa agatctgttt cgtcgctgtg gtgtggtttg ctttagcagc aattatgagc
     1321 tttacgcaga catgagcaat cgggttatgt cgacgctgga agaactatcg ccccgcgtcg
     1381 agatttacag tattgatgag gcattctgcg atctgacagg tgtgcgtaat tgtcgcgatc
     1441 tgactgattt tggcagagaa attcgcgcaa cggtgctaca acgtacccat cttactgttg
     1501 gtgtggggat cgcccagacc aaaacgctgg ctaagcttgc caatcatgcg gcaaaaaaat
     1561 ggcagcggca gacggggggg tggtggattt atcaaatctg gaacgccagc gtaaattaat
     1621 gtctgctctc cccgtggatg acgtctgggg gattggacgg cggatcagca aaaaactgga
     1681 cgcgatgggg atcaaaaccg ttcttgattt ggcggataca gatatccggt ttatccgtaa
     1741 acattttaat gtcgtgctcg aaagaacggt gcgtgaactg cgcggtgaac cctgtttgca
     1801 actggaagaa tttgcaccga caaagcagga aattatctgt tcccgctcgt ttggtgaacg
     1861 tatcacggat tatacgtcga tgcggcaggc catttgtagt tacgctgccc gggcggcgga
     1921 aaaacttcgc agtgagcatc aatattgtcg gtttatctcc acatttatta agacgtcacc
     1981 atttgcgctc aatgaacctt attacggcaa tagcgcgtcg gtaaaactgc tgacgcccac
     2041 tcaggacagc agggatatca ttaacgccgc tacgcgatct ctggatgcca tctggcaagc
     2101 gggccatcgc taccaaaaag cgggcgtgat gctgggggat ttcttcagtc agggagtcgc
     2161 gcagctcaat ttattcgatg acaacgcacc gcgccccggg agtgagcaat tgatgacggt
     2221 aatggataca ctgaatgcta aagagggcag aggaacactc tattttgccg ggcaggggat
     2281 ccaacaacaa tggcagatga agcgagccat gctttcacca cgttatacaa cgcgaagttc
     2341 tgatttactg cgggtcaaat aaatatagcg gcaggaaaaa agtgctcccg caggagcgcc
     2401 gaatggatta gcgaccgaac agatcacgtt ttttcgcttt aaacggctgg gaaatcatca
     2461 ccagcaccgc gacaatcagg taagcgataa aaataccgag cagccactgc ggcatttcca
     2521 gacctaaaaa atcccactga cgctcggcgc aatcgccaga ggcgacaaac acttgcggca
     2581 cccatttatc cagcggcagc cattccggga aacgaaccat aaaatcacag gtggcaaacg
     2641 gcgaaggata gagctgaagc atggtgtgct cgtaagttaa ctgcacaccg cggaacgcac
     2701 tatacaacca gataaccatc gctacataac gcagcggagt tttcggggcg atcgcgccaa
     2761 tcagcgcagc acccagaacg ccgaataacg cgcagcgttc ataaatacag agcacgcaag
     2821 gtttcagtaa catcacatgc tggaaccaca gcgccgtcag ttccagtgcc agagcagtaa
     2881 acgccatcaa cagccacgcg ccccggcctt gtgaacattg gttcaaaaat cgcaacataa
     2941 tcatttccct gcaatatgca tagagtgcgc agtttaaacc aattcattcg ctgcgccacc
     3001 aggaggcagc aaaaatagga cgcaattgga caattatcgg gcgaaaaggc aaaccgggca
     3061 tttatatgcc cggtaagttg ttatcaaagc gttgctatcc agcccatttg cataaaccat
     3121 tcggttacag gggcaagcgt aaactcgacg cagagcagcc cgacgagtgt caggacgagg
     3181 gtgtaaggca gggccatcca caccatgcgg ccataagaga ggcgaatcaa tggcgcgagt
     3241 gcagaggtca gcaggaacag gaacgcagcc tgaccgttcg gcgtagcgac agagggcaga
     3301 ttggtaccgg tattaatggc gaccgccagc agctcgtatt gcttcaacgt gatagcgcca
     3361 ctttccattg ccgctttcgc ttcgttgata taaatcgtcc ccacgaagac gttatccgaa
     3421 atggatgaca gcagaccgtt gaaaatatag aacaacgaca gctgagcatg ttccgatgcc
     3481 tgcaacacaa actgaataat cggcgaaaac agttgttggt cgataatcac cgcgactacc
     3541 gagaaaaaca ccgtcaacag tgcggtgaat ggcagagatt cggtgaaggc tttaccgata
     3601 gcatgttcat cggtgacacc ggtcaatgat gtcgccagaa taatgaccga caaaccaatc
     3661 aagccaacct ccgccagatg caacgccagc gcagtcacca gccagacgcc gataatcgcc
     3721 tggacaatca gacggatttt atcctgacgg gtacgctggt ggcggctttg atcgtcaaac
     3781 tgttgcaaca cttcgcggac tttctccggc agcgtttcac cgtagccaaa ccaacgcagc
     3841 ttctctacca gcaggcaggt taacaggcca caaatcagaa ccggaacggt caccggcgac
     3901 atgcgcagga agaaatcgcc aaaatgccag ccagccgctt tagcgatgat caggttctgt
     3961 ggttcgccca ccatggtcat tacgccgcct aatgcggtgc cgacaccggc atgcatcatc
     4021 aggctacgca gaaagccacg gaactgttcc agaaccactt tgtaatgctt gtcgatatga
     4081 ctatcgtctt gcaggtcggt atcttcagta cgggaagagg ctacgcggtg ataaatacca
     4141 taaaaaccga ctgcaacgct gatcaccacc gccacgacgg ttaaggcatc gaggaacgcg
     4201 gagaggaacg ccgccgccac gcaaaaagag agcgacagca gcattttgga acgaatgctt
     4261 aacagcaaac gggtaaatat gaacagcaat agctgtttca taaaatagat acccgccacc
     4321 ataaacatca gtaacagcaa gacttcaaga tttgccgcca cctcttcacg gacgtgttcc
     4381 gcgctggtca tgccgatgaa taccgcttca atagccaaca gaccaccggg gagtagcggg
     4441 tagcatttca gggccatcgc cagagtgaaa ataaattccg ctaccagcaa ccagccagcg
     4501 acgaaagggc tgatgaggaa aattaacgga tttacgatta agaaaatgat gagggcgagt
     4561 ttgtaccagt cgggggactg gcccaaaaaa ttgcgccata gcgcgcggcc ccaggagatc
     4621 tccatgatgg tttcccttac cttacaaata atcaatgatg tttttatgtt taaacgcaaa
     4681 gcttaacggt caggcaggag tgaggcaagt cttgatagtc aaggggaaag agatgcggaa
     4741 aatgaagcct tgatcccttt tttcttcttt ttgtctgcta tcagcgtagt tagccctctg
     4801 gtatgatgag tccaactttg ttttgctgtg ttatggaaat ctcactatgg tcattaaggc
     4861 gcaaagcccg gcgggtttcg cggaagagta cattattgaa agtatctgga ataaccgctt
     4921 ccctcccggg actattttgc ccgcagaacg tgaactttca gaattaattg gcgtaacgcg
     4981 tactacgtta cgtgaagtgt tacagcgtct ggcacgagat ggctggttga ccattcaaca
     5041 tggcaagccg acgaaggtga ataatttctg ggaaacctct ggtttaaata tccttgaaac
     5101 actggcgcga ctggatcacg aaagtgtgcc gcagcttatt gataatttgc tgtcggtgcg
     5161 taccaatatt tccactattt ttattcgcac cgcgtttcgt cagcatcccg ataaagcgca
     5221 ggaagtgctg gctaccgcta atgaagtggc cgatcacgcc gatgcctttg ccgagctgga
     5281 ttacaacata ttccgcggcc tggcgtttgc ttccggcaac ccgatttacg gtctgatcct
     5341 taacgggatg aaagggctgt atacgcgcat tggtcgtcac tatttcgcca atccggaagc
     5401 gcgcagtctg gcgctgggct tctaccacaa actgtcggcg ttgtgcagtg aaggcgcgca
     5461 cgatcaggtg tacgaaacag tgcgtcgcta tgggcatgag agtggcgaga tttggcaccg
     5521 gatgcagaaa aatctgccgg gtgatttagc cattcagggg cgataatccc ttccgtttaa
     5581 aaagcaaacc cctcaaacga ggggtttttt gttgttttta cagatttccc attcttggcg
     5641 ggcaacgttc cagcaactcg acgctgccat cttcgttttg ctgttccagc atcacatcaa
     5701 atccccacag gcgatgcaca tgcttcagca cttctttgcg cccccgatcc agcggtgcgc
     5761 gattatgtgg aatgtaacgc agcgtcagcg aacggtcgcc gcgcaaatcc acgttccaga
     5821 tctgaatatt cggctccaga ttacttaagt tatattgcga cgataaccgg ttacggatct
     5881 cccgataacc ttcttcatta tgaatagcgg aaatctccag ataattatgc cgatcgtcat
     5941 ccagcacggt gaagaagcgg aaatcacgca tcactttcgg tgacagaaac tggctgataa
     6001 aactctcatc tttgaaatca cgcatcgcga aatgcagcgt ttccagccag tcggaaccgg
     6061 cgatatccgg gaaccagtat ttgtcttctt ccgttggcga ctgacaaatc cgtttaatat
     6121 cctggaacat ggcgaacccg agggcatacg ggttgatgcc gctgtaccac gggctgttat
     6181 aggggggctg gaagaccaca ttggtgtggc tgtgcaaaaa ctccagcata aaacgttccg
     6241 ttactttccc ttcatcatac agatggttaa ggatggtgta gtgccagaag gtggcccagc
     6301 cttcgttcat cacctgagtc tgtttttgcg gataaaaata ctggctcacc ttacgcacaa
     6361 tacgcaggat ttcacgctgc catgattcca gcagtggcgc atttttctcc ataaaataga
     6421 gcaggttttc ttgtggttcg gacggatagc ggcgcgcttc agcaaccgtt ttctcttcct
     6481 cgcgcttcgg cagggtacgc cagagcatat tgacctgact ttgtagatac tcttcgcgac
     6541 ttttctgccg ggctttctct tcttgcagcg agattttttg cgggcgtttg taccggtcca
     6601 cgccgtagtt catcagcgcg tggcacgagt ccagaagccg ttctacttca tccacgccat
     6661 aacgttcttc gcactcggta atatatttac gggcaaaaat cagataatcg acaatcgaac
     6721 tggcgtcggt ccaactgcgg aataagtaat tgtttttaaa gaaagagtta tgcccatagc
     6781 aggcgtgtgc catcaccagc gcttgcatgg taatggtgtt ctcttccatc aggtaagcga
     6841 tacacgggtt agagttaatg acgatttcat aggccagtcc ttgctgaccg tgcttataca
     6901 gccgttcagt ctcgataaac tttttaccga atgaccagtg cggatagtta attggcatgc
     6961 cgacgctgga gtaggcatcc atcatctgtt ctgaggttat cacttcaatc tggtgcgggt
     7021 aggtatccag ccggtagagt ttcgccaccc ggtctatctc tgccagataa acatccagca
     7081 ggtcgaacgt ccagtcgggt ccatcgctca accgtgtggt gtccttattc atagaatcga
     7141 tcgtcgccat acgcgcacct cattgttgtc ggcgctctct gtgtggagca cctcatttca
     7201 agcatagaac acctgttaaa aaccgcgtcg ccggagaatt tttttctttg cgatttctta
     7261 ttatcagagt gccactaatc cgcttctgaa cggaatttta tgctggataa aaagggcgtt
     7321 cagcaggaga tactaaagac gccatattgc cgcagagtca gggagatgtg agccagctca
     7381 ccataaaaaa gctgtatgtt gaataatatt ttcaactgag ttatcaagat gtaattagat
     7441 tattattctt ttactgtatc taccgttatc ggagtggcta tgcgagttgt catactggga
     7501 agtggtgtgg taggcgttgc cagcgcctgg tacttaaatc aggcaggaca tgaggtcacc
     7561 gtcattgatc gggagccggg ggccgccctg gaaaccagcg ccgcgaatgc cgggcaaatc
     7621 tcccccggat atgctgcacc gtgggcggca ccaggtgtgc ctttaaaagc gatcaaatgg
     7681 atgttccagc gccatgcgcc gctggcggtt cgtctcgacg gtacgcagtt tcagttgaaa
     7741 tggatgtggc aaatgttacg taactgcgac accagccact acatggaaaa caaagggcgg
     7801 atggtgcgtc tggcggaata cagccgtgat tgcctgaaag cattgcgcgc cgaaaccaat
     7861 attcagtatg aagggcgtca gggtgggacg ctgcaactgt tccgtaccga acaacagtat
     7921 gaaaatgcga cccgcgatat cgccgtgctg gaagatgccg gcgtaccgta tcagctgctg
     7981 gaatccagcc gcctggcgga agtggagcca gcgctggcag aagtagcgca caaactgacg
     8041 ggtggtctgc aactgcccaa tgatgaaacc ggagactgcc agctgtttac ccagaatctg
     8101 gcgcggatgg cagagcaggc gggggttaaa ttccgcttta atacgcccgt tgaccaactg
     8161 ctttgcgacg gcgagcaaat ctacggcgtg aagtgtggcg atgaagtgat taaggccgat
     8221 gcgtatgtga tggcgtttgg ttcttactcg acggcgatgc tcaaaggcat tgttgatatt
     8281 ccggtgtatc cgctgaaagg ctactcgctg actattccaa ttgcgcagga agatggtgcg
     8341 ccagtatcca ccattcttga tgaaacctac aaaatcgcca ttacccgttt cgataaccgc
     8401 attcgtgttg gcggaatggc ggaaattgtt ggttttaaca ccgagctgtt gcaaccgcgt
     8461 cgtgaaacgc tggaaatggt ggttcgcgat ctctatccac gcggcggtca tgtcgagcag
     8521 gcgactttct ggacgggttt gcgcccgatg acgccagacg gtacgccggt tgtcgggcgt
     8581 acacgcttta aaaatctgtg gctgaatacc ggtcacggca cgctcggctg gacgatggct
     8641 tgcggttccg gtcagttgtt aagcgatctg ctctctggtc gcacgccagc gatcccatat
     8701 gaggatctaa gcgtagcgcg ctacagccgt ggatttacgc catcacgtcc gggccattta
     8761 catggcgcac acagctaagg aaacgagatg acccgtccga tacaggccag cctcgatctg
     8821 caggcattaa aacagaatct gtccattgtc cgccaggccg cgccgcacgc gggtggtaaa
     8881 agcgaacgct tacgggcatg gtattgagcg tatctggagc gcgctcgggg ccaccgatgg
     8941 ctttgcatta cttaacctgg aagaggcaat aacgttacgt gagcgcggct ggaaggggcc
     9001 gatcctgatg ctggaaggat ttttccatgc tcaggatctg gagatttatg accagcaccg
     9061 cctgaccacc tgcgtacaca gcaactggca gctcaaagca ctgcaaaatg cgcggctaaa
     9121 agcaccgttg gatatttatc ttaaagtgaa cagtgggatg aatcggttgg gcttccagcc
     9181 cgatcgcgtg cttaccgtct ggcagcagtt gcgggcaatg gcgaatgttg gcgagatgat
     9241 cctgatgtcg cattttgccg aagcggaaca tcctgatgga atttccagcg cgatggcgcg
     9301 tattgagcag gcggcggaag ggctggagtg tcggcgttcg ttgtccaatt cggcggcgac
     9361 tctgtggcac ccggaagcgc attttgactg ggttcggcct ggcattattt tgtatggcgc
     9421 ttcgccgtcc ggtcagtggc gtgatatcgc caataccgga ttacgtccgg tgatgacgct
     9481 aagcagtgag attattggtg tccagacgct aaaagcgggt gagcgtgtgg gctacggcgg
     9541 tcgctatact gcgcgcgatg aacagcgaat cggcattgtc gccgcagggt acgccgacgg
     9601 ttatccgcgc cacgcgccta ccggtacccc tgttttagtg gacggcgtgc gcaccatgac
     9661 ggtggggacc gtctcgatgg atatgctggc ggtcgattta acgccttgcc cgcaggcggg
     9721 tattggtacg ccggttgagc tgtggggcaa ggagatcaaa attgatgatg tcgccgccgc
     9781 tgccggaacg gtgggctatg agttgatgtg cgcgctggcg ctacgcgtcc cggttgtgac
     9841 ggtgtaactt gttgtaagcc ggatcggagg caacgtcttc tgggtgcaaa aaaaatcatc
     9901 catccggctg gtcagcaact gtagttgtta atgtgacaga gccattgccc atgatagtgt
     9961 ccattaaaag gatggacact atttccccgg aacctgaact caccgcacag gcgttctaca
    10021 taaaacgctt acgcttcatt gttgactcgg agtcgacttc agataaatca cgctttaccc
    10081 ttgatggagc ctgtacatag atttgtgtaa ttgcctgatt ttgatatgtt caattcaaca
    10141 tcaaatgaag gttaaattat ggacgacaaa caattgcagg ctcaggctgc gttcagcaaa
    10201 gcatcgcaac cggcgataga tgcttcatta aatttaagat tcagcttcct cttcagccac
    10261 ccgtacgcca atcttcaaca cttcattatc ttctttctcg gccaccgtcc agatcatccc
    10321 ggcaaactct acctggtcac cgacaaccgg tgccgtgcct aacaactgct ggacaatttc
    10381 acccagcgtt tgctgcttat cacgatattc tcgcccgtct tccaggccat atatcagcgc
    10441 aacatcagca tatttggcgc tggcttcgag aatgaagtca ccaaagaagc gttgatccag
    10501 cgcgaccggc ggcgactggc tgaacagttt accgagcgcc ggaagatcgc gttcccgacc
    10561 aattacacac aacacatcgc cttcgcgcag tcgggtgctg ccggtaggat gaagcaactg
    10621 gttatcacga aacagtgccg caatacgcgt ctcttttggc atatgcaaat cgcgcagtgc
    10681 cgcgcccacg caccatttat cggcactcaa ttgataaaca aactgctccc acggattttc
    10741 cggatgaata tccaggccaa cgcgtgacac cggacgtccc actggcggaa ccaccacttt
    10801 ggctttttta gccgcccacg agagtgatgt tccctgcaag agcagtgaaa ccagaaccac
    10861 aaagaaggcg acattaaaga acagacgtgc attctccagc cccgccatca tcgggaacac
    10921 ggccaggatg attggcaccg cgccgcgtaa tcctacccag ctgataaaca cgcgctcacg
    10981 cagattgaaa ccgcggaagg ggagcaatcc agcaaatacc gaaaggggac gggcgaagaa
    11041 tatcatccat gccgacaaaa ttagcgccgg aatggcaata ggcagcagat cgcttgggtt
    11101 aaccaatagc cccagcacca ggaacatggc gatttgcgcc agccaggcga ggccgtcgaa
    11161 attttgcagg atgccgtagc ggttgcgaat cggccgatta cccagcagaa aaccgcacag
    11221 ataaacagcc agaataccgc tgccttccag cgccgtggtt aaagcaaaaa ttaaaatacc
    11281 gccgcttaat gccagcaatg gatataatcc ggcaggcagg gcgatgcgat taatcatttg
    11341 cagcagtaaa taaccaccgc caagtccgat gacaatcccg aggccaaatt gctgcagaat
    11401 atcgaccacg aacatccaac tgacactgct ttcatgttgc tggatcatcg caatcagggt
    11461 aatcgtcaga aagaccgcca ttggatcatt actgccggat tcaatttcca gcgtcgagcc
    11521 aacacgttcg ttaagcccct taccacccag cagagaaaag accgctgcag catcggtaga
    11581 gccgacgata gcgccgatta ataagccttc aatcaaatca agattaaaca gccacgccgc
    11641 catcatgccg gttaaaccag aggtgataag cacgcccagc gtcgccagcg acagtgccgg
    11701 tcctaacgcc acacgaaagg aactggcctg agtgcgcatc ccgccgtcga gcaaaataat
    11761 cgccagtgcc aggttactta ccatgtaggc gaaggggtaa ttatcaaacg ggataccgcc
    11821 gacgccatcg actcctgcca gcatgccgat cgccagaaaa ataaccagaa taggaatgcc
    11881 aagacgggaa gaaaatgaac taagtaatat actgctggtg acaaggatgg atcctaagat
    11941 gaaaaggcta attattgttg tggcatccaa cggtcggtta ctcctgatta cgctgtctct
    12001 tatataaacc ctaccatatt agcggcagag acagcgtttt acttagccct gaagcgtgat
    12061 ttttttacat tttaagaaca ggatgaccgc taatcgtcaa ctgagtgcct tcccgggtgt
    12121 tattcagaat ggcatgtgcg cccaacggca gcgtaaccgt gcgttgttca tgaccaaaat
    12181 cgaggccggt aatgagtgga atcgacaggc gggaacgcaa aaacgcggac actgactcca
    12241 ggttgtaacc tgcgtcataa tcattgggcg tgctgccgct aaagctaccg agaataatcg
    12301 ccttctgacg tggcaaaatt cccgcatgat agagctgcaa cagcatacgt tcgacgcgga
    12361 aagggtgctc gttaatatct tccagtacca ggataccgtt ctcaattttt ggcatccacg
    12421 gtgtaccaat cagtgaaatc agcatcgcaa gattgcctcc ccacaacgtg ccttcggtct
    12481 gacatgtcgg gccttcccct tgccattcaa tggtgaaggt tttattgcgt aacgccagcc
    12541 agaaatggtg ctcggtaaag gcgttcagtt catccgcacc aaagtttgct accagcatgg
    12601 ggccgctaaa ggtgatgaca ttgccttgcg ccagaagacc gcactgaatg gcggtaaaat
    12661 cgctatgtcc gcaaatgagt aacgggtcat gttgttggcg agccaccagt gcctgccagt
    12721 caatatctgc cagtaaacga ctggcaccgt aaccgccgcg tacagccagc acgatggtgt
    12781 tgggtgtagt cagtctggca agggaattga gatcttccag acgctccgtt tccgtacccg
    12841 caaaacgctc acaacgacgg gcaataacct cgacgttatt gacttgatgc cccgcgtcgg
    12901 taaggcgctg gataccacgc agcgcggcgt gctgtttaat gctgtaaccc gatggggcaa
    12961 ttaagtgaaa cagagacatg gcaattcctt gctgacaaca gaaacgaaat gtatatcatg
    13021 ccgcttaggt gtgccgttgt cacctcaacg gcgattccag gctataagga tagaagaagt
    13081 gaaattgaga tggtttgcct ttttgattgt gttattagcg ggttgttcat caaagcatga
    13141 ctatacgaac ccgccgtgga acgcgaaagt tccggtgcaa cgtgcgatgc agtggatgcc
    13201 aataagccag aaagccggtg cagcctgggg cgtcgatcca caattgatca cggcgattat
    13261 cgctatcgaa tcgggtggta atccgaacgc ggtgagtaaa tcgaatgcca ttggtttgat
    13321 gcagttaaaa gcttcaacct ccggacgtga tgtttatcgc cgtatgggct ggagtggtga
    13381 gccgacgacc agcgagctga aaaatccaga gcgtaatatt tcaatggggg cggcttacct
    13441 gaatattctg gaaaccggcc cgctggcagg cattgaagat ccgaaggtac tgcaatatgc
    13501 gctggtggtg tcatacgcta acggggcagg tgcgctgcta cggactttct cgtcagatcg
    13561 gaaaaaggcg atcagcaaaa tcaacgattt ggatgctgac gagttcctcg atcacgtagc
    13621 gcgcaatcat cctgcgccgc aggctccgcg ctatatctac aaacttgagc aggcactgga
    13681 cgcgatgtaa atcagtcgcg tactttgtcc gctttctccc gggcttcccg ctcgagagag
    13741 aaaataatcc gttgtaattg ccgctccacc gtcgggctga cgttaagaaa acggaagctc
    13801 agacggggag tggtgatggt ttcattcttg ccatcaatca ctttgcgctc gctgatggag
    13861 attaactggg cgtcaaagtg aaaaacaccc cattgcccca tgttgacttc aatctgagcg
    13921 aagcgcatgc cttcttgtaa ttcggcaggc tttgctgttt ccagtaatgc gcccatgccg
    13981 cctaacgaca aatcatacag gcggaaacgt aacgtactgt tatccgccag tttggtctgg
    14041 caaaaataag gcggatggag tggggcggag atgcggaaat atcggcgtcg ttgtacaaac
    14101 cataaggtgg gaggcggtac ggtaataaat gccggaagct gcaagtattc actctgctgt
    14161 agttgttcaa cagtaaactc gactttcgca ccctgagttt cggcggtaat ggtaatatgc
    14221 tgcgccttta gcacggcgat gttgtcttcg gcttgactgc cgaaatccag caccagttta
    14281 tccggggtta ttgccagtat tttgctgatc agctgcccgc cattccaact gagacgcaaa
    14341 ggaattgcgg ctttgtgcaa atcgcgtaac acgcccagga cggctaacgg attttgtttc
    14401 aggaactgct catggtaatg actcacgcgg aaaaactcct gactgacaaa ctgtctttgg
    14461 tttatcggtc acagttaaca aaacttaata caaatgcgtg aatatttttt acatgttgtt
    14521 cttaaatcag ccgacatacg cccagcatcg atcccgccct tgcctatact tagagcgttg
    14581 acgtaagcat ttcttgcgtc cgattcatcg aaacgagggc ttgaacatgg gaattattgc
    14641 ctggattatt tttggcctga tagccggcat tatcgccaag ctaatcatgc cggggcgtga
    14701 tggtggtgga tttttccaga cctgtattct cgggatagtc ggtgcggtgg tcggcggctg
    14761 gctggcgacc atgtttggca ttggcggctc catcagcggt tttaatttgc acagcttcct
    14821 ggtggcggtg gtgggagcta ttctcgttct gggcttattc cgcctgctgc gaagagaata
    14881 agattttcat aaggcggata gcgatacaga tgccgctatc cgcttttcac atcagaacgt
    14941 atattcgaca cctgctttac gggtgaaaaa aatcaatata gcacttcgat atccgataaa
    15001 gtcggttccc ggtaacaaca acagaatcct tttttaatta ttgtttcgtt gtttttggtg
    15061 tgattcagaa aataaatatt ttagacagta gccagtcagg cgttaaaaat ggggcggata
    15121 gtgaggcggt tttggctgcg cttattgaac atttagaatt gatcaatcct tctggcagat
    15181 taagccaaaa tacccgttcg gcaatgcttc agttacgcga agaggaatgg tctgaattct
    15241 ttttttggtt gctaaattct ctggagtgtc tggattatgt gattattaat ttaactccag
    15301 aaagcaaaaa aacgttgatg agtgaacacc gtaacaatat tcaggtagca atcgatgctt
    15361 tatataggca acgtcggtgt aaatcaccgg gggatgatag tgaaacactt acccgtagaa
    15421 atgacgctat cttcggcaac cacgtatggc aaacatttgc gcaatacttc ccgccaggac
    15481 ttgaaaaacc ggctgtgtag aaaacaccat agctatggtg ttatctgccg acaacgccat
    15541 ccggagaagc cgaatgacgt tgtcggagta agcgctggtt aaggtgtggg ttgtgcctct
    15601 ttggttgagg gttgcgtcgt tgctgactta acggtcggac gcgtcgccgg aacattgtca
    15661 cacggttgct ctttcgggca gatcaaatcc agcattttca gcgtcacgcc attggtccag
    15721 ccaaagccat cctgtaatgg atattcgcca cctctttttg cccgtagttt tgtaatcctt
    15781 ctgtcgcgac ccactgtaac ggtgcccagc catttggcgc atcccattgt tgcccacttt
    15841 tcaccgacgt ggtgttcagg ccgccgggtt gcagcagatg tgttttcgtc gccgtcgcca
    15901 ttttgttggc gcgatctttc gctgccgcat tgacgtacag cgggaacagg gcggccgcgg
    15961 ttaactgatt gcgcacttta tgacttttca ggtcgtaatc ggcataccag ccttgttgat
    16021 cgttccacag gtatttttcg attccttttt gacgggcatt cgccagcgtt tcgtactggt
    16081 ttgccatcgc gttatctcct gcagctttgc tggcgcgggc gaggattttt tccattttaa
    16141 acatcaggct gttcagatcg accggtacga tgctggtggt gcgtaaggta tttaactgct
    16201 gcgggttgtc catccagcgc gagctgaaat cccagccaga cgcagcggca gagcgcaggt
    16261 cgcggtaaat ttcagtggca ggtcgattcg gattgctttt ggcggtggca atatcttcca
    16321 cccatgactc tggtcgtggc gtatcgcgat cgtcccagta gcggttgaga agggtaccat
    16381 cctgaagttt gacaacgcgt ttttcctgtt gtccggcttg caggttttca acaccgtcca
    16441 tccagtaagc atattctttt tgcatttgcg gcaggtattg cttcaacgcg gcatcgcctt
    16501 catgctgcgc cagtaactct accatcaggg caaagaaggg cggttgcgag cggcttaaat
    16561 agtaactgcg gttgccgttg ggaatatgac cgtaagtgtc tatttcatga gcaaaattgg
    16621 ccaccatatc cgcgacttta tcccagtgac cgctttcggc aagtcctaac atggtgaagt
    16681 aactgtccca gtaatatacc tcgcgaaaac gtccgcccgg cacgacataa ggttccggca
    16741 gcggtaacag agaatcccat ttttcggtgt tttcggtaga acgcgttaat accggccaaa
    16801 gtccgtcaat atgttcgcgc agtgactgcc cctctggcgg aacatatttc tcgccttctt
    16861 tcggcagggt gaaattgacg ttaacgaaat ggcgcagatc aaatccgctc tggttttgct
    16921 gcatccgata atcagcggat cgctgttcgg cacggcatcg gcaaaggttt tttggtccgg
    16981 aaaaagtttg gcgttttgca catcattaaa cagcggccct aataaaatat caggcggctg
    17041 tggtgttacc ggtgtttctt ctgcctgcac cgatagcgca gcgaaacaca aaaagataca
    17101 ggct
//
LOCUS       DADLFA010000085        16618 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000085 DADLFA010000000
VERSION     DADLFA010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16618
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.085"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(1..244)
                     /locus_tag="JQH65_002333"
                     /pseudo
     CDS             complement(1..244)
                     /locus_tag="JQH65_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100289.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
     gene            351..647
                     /locus_tag="JQH65_002334"
     CDS             351..647
                     /locus_tag="JQH65_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY6139724.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(781..1206)
                     /gene="arsC"
                     /locus_tag="JQH65_002335"
     CDS             complement(781..1206)
                     /gene="arsC"
                     /locus_tag="JQH65_002335"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HAY6139725.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(1219..2508)
                     /gene="arsB"
                     /locus_tag="JQH65_002336"
     CDS             complement(1219..2508)
                     /gene="arsB"
                     /locus_tag="JQH65_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HAY6139726.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(2563..2916)
                     /gene="arsR"
                     /locus_tag="JQH65_002337"
     CDS             complement(2563..2916)
                     /gene="arsR"
                     /locus_tag="JQH65_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HAY6139727.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRN
                     LARQNCSADSKNICS"
     gene            3655..3738
                     /locus_tag="JQH65_002338"
     CDS             3655..3738
                     /locus_tag="JQH65_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HAY6139728.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3792..5144)
                     /gene="gorA"
                     /locus_tag="JQH65_002339"
     CDS             complement(3792..5144)
                     /gene="gorA"
                     /locus_tag="JQH65_002339"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAY6139729.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKMLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSEHLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(5216..6058)
                     /locus_tag="JQH65_002340"
     CDS             complement(5216..6058)
                     /locus_tag="JQH65_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HAY6139730.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            6261..8303
                     /gene="prlC"
                     /locus_tag="JQH65_002341"
     CDS             6261..8303
                     /gene="prlC"
                     /locus_tag="JQH65_002341"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HAY6139731.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTQALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            8311..9063
                     /gene="rsmJ"
                     /locus_tag="JQH65_002342"
     CDS             8311..9063
                     /gene="rsmJ"
                     /locus_tag="JQH65_002342"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HAY6139732.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVTTPNAVVTKGHRFDIYAGTPV"
     gene            complement(9112..10581)
                     /gene="dtpB"
                     /locus_tag="JQH65_002343"
     CDS             complement(9112..10581)
                     /gene="dtpB"
                     /locus_tag="JQH65_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HAY6139733.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINSVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(10899..11333)
                     /gene="uspA"
                     /locus_tag="JQH65_002344"
     CDS             complement(10899..11333)
                     /gene="uspA"
                     /locus_tag="JQH65_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HAY6139734.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11724..12059
                     /gene="uspB"
                     /locus_tag="JQH65_002345"
     CDS             11724..12059
                     /gene="uspB"
                     /locus_tag="JQH65_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HAY6139735.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(12302..13801)
                     /gene="pitA"
                     /locus_tag="JQH65_002346"
     CDS             complement(12302..13801)
                     /gene="pitA"
                     /locus_tag="JQH65_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HAY6139736.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKIPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            14033..15235
                     /locus_tag="JQH65_002347"
     CDS             14033..15235
                     /locus_tag="JQH65_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAY6139737.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(15550..16602)
                     /locus_tag="JQH65_002348"
     CDS             complement(15550..16602)
                     /locus_tag="JQH65_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HAY6139738.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNILFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGYVIFGIGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILIYLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
BASE COUNT         4267 a         4237 c         4314 g         3800 t
ORIGIN      
        1 caatccacgt aacatgggta ttgtcgagca actacagaac ggggtaacgg ctcccaacag
       61 agcagccagg agatagcctc tcccttttct ggcccccatc atctgctgga ttttatggtc
      121 tggcaccttt tgtcttatca ggctgacacc ggcactaatc actataaaca gcaacgaaag
      181 ctcgacggca gagaaacaca aacatctctg cggcatcttg cagcatcgca agccaactac
      241 tcatacgtaa cctcattgga taaatctata tttccagaat aatcgaaatg aaattcagat
      301 caagatattt ctggtattat cgaaatatca accagacaga ggggtgtcat atgcagttgg
      361 aagaagtcgc taaagcgctg aaagagctag gtcacccgac ccgtttgttc atatttaaac
      421 atcttgtcaa agccggggaa caagggttgc cggtcggtga gttgcaaaag cagcttggga
      481 tacctggctc cacactcagc caccatatat cagcactggt atcggtagga ctggtcaaac
      541 aaaaccgtga gagtcggacg ctgatgtgtg tttcgcaata cgcaatgctg gaagcgataa
      601 ttgaattctt acgcgaggag tgctgcgtaa atagcaaaat tgcctgatgc gctacgctct
      661 acatatttca tgccatatat tgaatttgca cggttttgta ggtcggataa gacgcgttag
      721 cgtcgcatcc gacataaaca aagcgcactt taccaactat cggcagtgga tatcgccgct
      781 ttatttcagg cgcttacccg cttcatcaac cactttctcg ccatcttcct tggagaatgc
      841 gcctttttgc gcatctggca gaatttccag caccacttct gaagggcggc acaggcgagt
      901 tcccagcggc gtcaccacaa tcgggcgatt aatcagaatc gggtgctgaa gcataaagtc
      961 gattaaccga tcgtcagtaa atttatcttc cgcaaggccc agctcctcat acggttcgac
     1021 gtttttacgc agcagcgcgc gtacggaaat ccccatatcg gcaatgagtt tgaccagttc
     1081 atcgcgcgtt ggcggagttt ccagataatg gataatggtc ggttctgtgc cgctgttgcg
     1141 gatcatctcc agcgtattac gcgacgtgcc gcaggccggg ttgtgataaa tggtaatgtt
     1201 gctcatatca gtatctcatt acaaagtgaa agagagacgt agcgccagcg cagccagcgt
     1261 cacaaacagc acaggcaggg tcatgataat ccctgtacgg aagtaatatc cccagctgat
     1321 agtcatattc ttctgcgaaa gtacgtgcag ccagagtagt gtagccaggc taccaattgg
     1381 ggtaattttc ggtcccaaat cgcagccaat cacattggca taaaccatcg cttctttgat
     1441 aacgccagat gccgtgctgc catcaatgga caacgcgcca accagtaccg tcggcatatt
     1501 gttcataata gaagagagga aggcggtgag gaatccggtg ccgagcgtcg cggcccacag
     1561 gccgttatcc gccagcacgt tgagtacgcc agaaagatat tccgttaatc cggcattgcg
     1621 caggccataa accaccagat acatgccgag cgagaagatg acaatttgcc agggggcacc
     1681 gcgcaggact ttacccgtat taatcgcatg accgcgttta gcgacgacaa ataatatcag
     1741 cgcgcccaca gctgcaatgg cgctcaccgg aatgccgagc ggttccagga cgaaaaatcc
     1801 caccagcaga agcagtaaaa caacccagcc agttttgaac gtagcaggat ctttgattgc
     1861 ttctgcggga gatttcagca gcgccatatc gtagttctgc ggaatatctt tgcgaaaata
     1921 gagatgtagc atcaccagcg tggcaacaat cgcggcgata tccaccggca ccatcaccga
     1981 ggcgtattcg cgaaagccga ggccaaagaa atcagcggaa acgatattca ccaggttgga
     2041 gacaataagc ggcaggctgg cggtatcggc aatgaatccg gccgccatca cgaacgccag
     2101 cgtcgtgcct ttactgaatc ctaaagccag cagcatggcg atgacaatcg gcgtcaaaat
     2161 aagcgctgcg ccatcattgg caaacagggc ggcaacggca gcaccgagca ggacaatcca
     2221 ggtaaacagc aagcgaccac gaccattacc ccagcgtgag acgtgcagcg ccgcccattc
     2281 aaaaaagccg gactcatcca gcagcaggct gatgataatg acggcgataa acgcagccgt
     2341 cgcgttccag acgatattcc acaccaccgg aatatcaccc ggatggacca cgcccgtaac
     2401 taacgccagt actgcgccga gcgttgcact ccagccgatg cctaaacctt tcggctgcca
     2461 gataaccaat acaatggtca ggacaaagat agcgcctgcc agtaacataa tgcctcctgg
     2521 ataaaacaca tgcgaaaata catatgtgtt tagctaaatt ttttaactgc aaatgttctt
     2581 actgtctgcg gaacagtttt gtcgagccag gttgcggaca attgcctgaa tcttttcctg
     2641 ttcacatcgc caggcttcat caattatttt cgccgcccat gctggaatat gcggcgataa
     2701 gcggtaatga acccatttac cttgcttgcg gtccagcaat agcccgcttt cacgcagcaa
     2761 tgccaggtgg cgggagatct tgggctgcga ctggtcgaga gcagtgcaga gatcgcagac
     2821 gcataactct cccagttcgc tgagcagtaa aacgatgccc agacgggttt catcagcaag
     2881 aattttgaac aattggatgg gtaacagaaa tgacatattg cgctcctgat tgttgcaggt
     2941 agtgtctctc ttcgaagcgg ataagtcaaa aacatatatg atttaacgaa tgtgtaagtg
     3001 cagaggaagg taataggtgt gaattttgag ttggctattc atttgaaagg aggtctgaat
     3061 caggacgtaa aaaaaccgac tttgcgtcgg ttttttactt tccagccctg agttggtggc
     3121 tctggctgga gtgagagagt tcttatctaa cagctcaata ctattagcca gtggctaaca
     3181 ttgcaagaat tagcgatttc ttcagctggc ggagggagca cttcggtata tatcgcagtg
     3241 acgatttata tactttcact gggtcatcgt catattaagc cttgtccgaa tccggatgta
     3301 aaaaaccgac tttgcgtcgg ttttttactt tccagccctg agttggtggc tctgactgga
     3361 gtgagatagc catcatctaa cgcatcaatg ctaataccat actgaaacta ttgcaaggac
     3421 gtgctggttt tataacctgc atgtactgta tgattatcca gttagctctg aggcattttc
     3481 actctggcaa tgcgcataaa cgctttcaaa gtcctggtca gaagtacggg tggtgccgtt
     3541 aactgatgct ctggccggag tgagagagtt cttatctaac aatgagacat gcgccgtgac
     3601 aggcagtgga tgagtaagcg gatgcattct cactccatcg catggagaaa acgggtgatt
     3661 gataaagcaa tcatcgttct aggggcgtta attgcgctgc tggaactgat ccgctttctg
     3721 cttcagcttc tgaactgata gcggaaacgt aattaagggc taagagcaca ctactcttag
     3781 ccctttaaca tttaacgcat tgtcacgaac tcttctgccg ccgttgggtg aatggcgacg
     3841 gtattgtcga agtctttttt ggttgccccc atcttcagcg ccaccgcgaa gccctgcaac
     3901 atttcgtcca taccaaagcc aatgccgtga ataccgacaa tcttctcttc cgatccaacg
     3961 cacaccagct tcatgcggca cggctggcgg tgagtggtga cggcggtata catcgcggtg
     4021 aaagaggatt tatacacttt cacctgatcg tcgccatact gctcgcgcgc ctgcggttcc
     4081 gttaaaccaa cagtaccaat cggcggatgg ctgaagacca cggtcggaat gttgctgtaa
     4141 tccagatgct catccggctt gttattaaac aggtgttcag agagacggcg acccgctgca
     4201 actgccaccg gtgtcagctc cactgcaccc gtgttatcgc ccaccgcgta aataccttca
     4261 atgttggtgt tttgatattt atcgacgacg atatagcctt tttcgttggt tttaacgcca
     4321 gcggcttcga ggttgatgtt gtcattggca ggctcgcgac caatcgccca aatcaggcaa
     4381 tccaccgttt cactgcgacc atcttccagc tccagcgtca ggctaccatc ggcatttttc
     4441 actaccgctt tcgggatggc gttggtgtgc agctgcgggc cttcggcgtt catcacttcg
     4501 accagcgttt cggaaatcat cgggtcgaag ctgcgcagcg gcgcatgttt acgcacaaac
     4561 agatgcgttt tcgcgccgag gccgttaatc acgcccgcca gctcaacggc gatgtaaccc
     4621 gcgccaacaa ccgccacgcg ctctggcaaa gcaggaaggg cgaagaagcc atcagaatca
     4681 ataccgtatt ccacgcccgg aatatccggg tggctcggac gaccgcctgt ggcgatcaga
     4741 atatgatcgg ccgtgatggt ttcgccgttt acctccagca ttttggcatc aacgaagcgg
     4801 gcaaagcctt tgattacatc aacgttattt ttaccgagca cgttttcata ggaagtatga
     4861 atacggtcga tataggcggt acggctggcg atcaacgttt cccagttgaa cttgttgatg
     4921 gtggtatcga aaccataatc agggccgtac atatggatcg cttcacggat ttgcgccgcg
     4981 tgccacatca cttttttcgg cacgcagccg acgttcacgc aggtgccgcc cagctctttg
     5041 gcttcaatca gtgcgcattt ctggccgtac atagccgcgc ggttgatgga ggctataccg
     5101 ccgctgccgc cgccgatggc gatgtaatca tagtgtttag tcatgacaga gtgtccttat
     5161 cgttgattac cgcgattgta gcgcgagacg taataggtgc cagcaatggc tgcaattact
     5221 ccggcacgat ccagcttacg gtggcgtgcc cggtgcctgc cggaaccagt ttgctgtgca
     5281 gccacggcag cacgttattc atctgctgtt ccagtttcca cggcgggtta atcacaatca
     5341 tgccggaagc ggtcatgcca cggcgatcgc tgtctggcag taccgccagt tcaatttgca
     5401 gaattttgcg aataccggtc gcttccagat cgtggatcat gcgcttaatt tgctgacgca
     5461 gcaccaccgg ataccacagt gcgtaagtac cagtggcgaa acgtttgtaa ccttctgcta
     5521 tcccgctgac caccgcctga tagtcggttt tcatttcata cggcgggtcg ataaggatta
     5581 aaccacggcg ggaaaccggc ggcagtttgg ccttaagctg ctggaaacca tcggcttttt
     5641 cgacgcgcgc acggctgtct ttctgaaatt cagaacgcag caacgggtaa tcgctcgggt
     5701 gcagttcggt cagttgcagg ctgtcttgtt cgcgcagtag ctggcgagca atcaatggcg
     5761 aaccggggta gtaacgcaac tgaccgctac ggttgaagtg ttttaccaca ttgatgtacg
     5821 cctccagttc tgcgggcaaa tcgtcctgct gccagatacg ggcgatgcct tcgagatatt
     5881 cgccggtacg ctcggcatgt tcgctgccta actgataacg cccggcccct gcgtgggtgt
     5941 cgagatagag aaacggttta tctttctctt tcagcgactc gatgatcagg ctctgaacgg
     6001 tatgtttaag gacgtcggcg tggttgccag cgtgaaagct gtggcgataa ctgagcatgg
     6061 gtaaaggtgt tccggtaagt aaaaatcggc ccgtattcgg ggcgcacaaa agcgtatcag
     6121 gacagtatac cgaaaagagg ccgccgccgc gaaagcgtaa cgtttctcat tgaaattcac
     6181 tacacttaac cccatgctac acacattatg taaagcgcct gttgagcgct tccttaacct
     6241 ctttaaccag gactgcgcga atgacgaatc cgttactgac tccctttgaa ttgcctccgt
     6301 tttctaaaat tctcccggaa catgtcgttc cagccgtgac tcaggcgctg aacgattgcc
     6361 gcgaaaatgt ggagcgcgta gtagcgcaag gggcaccgta cacctgggaa aatctctgcc
     6421 agccgttggc ggaagtggac gatgtgttgg ggcgtatctt ctccccggtc agccacctga
     6481 actcggtgaa aaatagcccg gaactgcgtg aagcctacga gcaaaccctg ccgctgctgt
     6541 cggaatacag cacctgggta gggcaacatg aagggctgta taaagcgtac cgcgacctgc
     6601 gcgatggcga tcattacgcc acgctgaaca cggcgcagaa gaaagcggtt gataacgccc
     6661 tgcgtgattt tgaactctct ggcataggtc tgccgaaaga gaaacagcag cgttacggcg
     6721 aaattgcgac ccgtctttct gaactgggca accagtacag caacaacgtc ctcgatgcga
     6781 caatgggctg gaccaaactc gttaccgacg aagcggagct ggcggggatg cctgaaagcg
     6841 cgctggctgc ggcaaaagcc caggccgaag cgaaagagct ggaaggctac ctgctgacgc
     6901 tggatatccc aagctacctg ccggtaatga cctactgcga caaccaggcc ttgcgtgaag
     6961 agatgtatcg cgcttacagc acccgcgcct ccgatcaagg cccgaacgcc ggtaaatggg
     7021 ataacagcaa ggtgatggaa gagatcctcg ctctgcgtca cgaactggcg caactgctgg
     7081 gctttgaaaa ctacgccttt aaatcccttg ccactaaaat ggcagaaaac ccgcagcagg
     7141 tgctggattt cttaaccgat ctggcaaaac gcgcgcgtcc gcaaggcgaa aaagagctgg
     7201 cacaactgcg cgccttcgcc aaagcggaat ttggcgtcga tgagttgcag ccgtgggata
     7261 tcgcgtacta cagcgaaaaa caaaaacagc acctctacag catcagtgac gaacagctgc
     7321 gtccgtactt cccggaaaac aaagcggtta acggcctgtt tgaagtggtg aaacgtattt
     7381 acggcatcac cgctaaagag cgtaaagatg ttgatgtctg gcatccggat gtacgtttct
     7441 tcgaactgta tgacgagaac aacgaactgc gcggcagctt ctacctcgat ctgtatgccc
     7501 gtgaaaacaa gcgcggcggg gcgtggatgg atgactgcgt aggccagatg cgtaaagctg
     7561 acggttctct gcaaaaaccg gtcgcgtatt tgacttgtaa cttcaaccgc ccggtaaatg
     7621 gtaaaccggc gctgttcact cacgacgaag tgatcaccct gttccacgag ttcggtcacg
     7681 gcctgcacca tatgctgacc cgcatcgaaa ccgctggtgt ttccggtatc agcggtgtgc
     7741 cgtgggatgc ggtcgaactg ccgagtcagt ttatggaaaa ctggtgctgg gagccggagg
     7801 cgctggcgtt tatctctggt cactatgaaa ccggcgaacc gctgccgaaa gagttgctgg
     7861 ataaaatgct ggcggcgaag aactaccagg cggcgctgtt tattctgcgc cagctggagt
     7921 tcggcctgtt cgatttccgc ctccatgccg agttccgccc ggatcagggt gcgaaaatcc
     7981 tcgaaactct ggcagaaatc aagaaactgg ttgccgtagt accgtctcca tcctggggcc
     8041 gtttcccgca cgctttcagc catattttcg ccggtggtta tgccgcaggt tactacagct
     8101 acctgtgggc tgacgtactg gcggcagatg ccttctcgcg ctttgaggaa gagggcattt
     8161 tcaaccgtga aaccgggcag tcgttcctcg acaacattct gagccgtggc ggttcagaag
     8221 agccgatgga tctgttcaaa cgcttccgtg gtcgtgaacc gcagctggat gcgatgctgg
     8281 agcattacgg cattaagggc tgatcattca gtgaaaatct gcttaattga tgaaacaggc
     8341 gccggagacg gcgccttatc tgttctggcg gcccgctggg ggctggagca cgatgaagac
     8401 aacctgatgg cgctggtgtt aacgccggaa catctggaat tgcgcaagcg tgatgagcca
     8461 aaacttggcg gcatctttgt tgattttgtt ggcggagcga tggcgcaccg acgcaaattc
     8521 ggcggtggtc gcggtgaggc ggtggcgaaa gcggtgggca ttaaaggcga ttatttgccg
     8581 gatgtggtgg acgccaccgc agggctgggg cgcgatgcct ttgtactggc ttcagtcggc
     8641 tgccgcgtgc ggatgctgga gcgtaatcca gtggttgccg cgctactcga cgacggcctg
     8701 gcgcgtggtt atgcggatgc ggaaatcggc ggctggttgc aggagcggtt gcagttaatt
     8761 cacgcctcca gcctgacggc gctaaccgat attaccccac gcccgcaggt ggtctatctc
     8821 gatccgatgt tcccgcataa gcagaaaagc gcgctagtga aaaaagagat gcgtgtcttt
     8881 cagtcgctgg tgggaccgga tcttgatgcc gatggattac tggagcctgc acgcttgctg
     8941 gcgaccaaac gcgtggtggt aaagcgcccg gactacgcgc caccgctagc gaatgtcacc
     9001 acgccaaacg cggttgtgac taaagggcat cgctttgata tctatgcagg tacgcccgtg
     9061 taaaagtaaa gcagggtagc gataacgcta ccctgctttc tgcaagaata attaatggct
     9121 ttccggcgtc gcaatcatgc gtttcagcca cggcaccatc aacagcatca ctactgcaac
     9181 gcccagcgtg accagaccaa tcttaccaaa cacgttggta tagacgggca acgtctcaag
     9241 cggatcggta atgttgtccg gcaccgcggt aaatgttgcc acatagccgc ccagcaagaa
     9301 cgcggcagcc tgcgtcagga accacatccc gagaataaag cccatcaaat gctgcggcac
     9361 cagggcagca atcatcgcca ggccaagggc gctaataaac agttcaccta agctctggaa
     9421 taagtacacc agcacgataa accatggcga tgtcagccct tgtgcatccg caaaccacat
     9481 tcccgcagct gccgccgtca aaaagcccag tgagcacata aacatgccga gagtaaattt
     9541 catcggcatc gagaggtctt tgcctttgtt acccagatgc gtgtaaatgc ctgccagtat
     9601 tgggctggcg agtaccaccc agaacgggtt aagcgcctgg aagctgaccg agttgatgga
     9661 aaaaccgaga atttcatgat gcacgttgtt gatggcaaag aagttcagcg atgttggcat
     9721 ctgggcgtag agaatgtaaa acaccaccgc ttcgagcatc aggacaaagg cgacaaacat
     9781 tttattgcgc ccggttttat ccagcttgaa tgcctgacga aagaagatga tggtgacgac
     9841 gatggagaga acaatcagca ccagattggc gacttctacg ttgtgcatca gccatgcaca
     9901 tacgaagatc atcaccacgc tgccaagtaa tacgtacaac agtttgctga agctcatcgg
     9961 cttgaagtcg ggttcagaac caatgtcttt caccattcca cgacaggcga tgtaaaccag
    10021 taatgcgata attaaccccg ccccgcacag gttgtaggtg actgaataac cgaatctatc
    10081 agcgatcaca ggggccagcg ataacgctat caacgagccg atattgatcg acatatagaa
    10141 cagggtgaat gcgccatcaa gccgcggatc tttcggcgga tagcacttcg aaagcaagct
    10201 ggctgggtta gctttaaaca ggccgttacc gacagcgata gtccccaggg cgatgaaaat
    10261 caggtcaggc ttaagtagcg acatgccggt catgaagtag ccaatcgcca gcacaagtgc
    10321 tccgagaaca atggtgcgtt tggtccccag caggtggtcg ccgacatagc cgccaatgga
    10381 aatgaggcca tagaccagcg cagcaaaagc accaaaagtg acaaaagcct gctcttgcga
    10441 gaatccaagc tgtttaacga agaaaaccgc cagtacgccc tgcacgccgt agtagccgaa
    10501 tcgctcccat aactcgacaa aaaagatcat gaaaaatggg cgaggttgct gcagcatccc
    10561 catgggtgtt gttgtattca tatttattaa ccttccaata ccatcctgaa aagtatgaac
    10621 gcgtaaggga taacgcgcga aaaagtttac atctgtcaga gattcttaat attgatgcgg
    10681 tgaatggatt aatacactat tacaacagaa aataaccagc taatcacact aatgatggaa
    10741 aatatgatgg tgttaaatgt tgtattggca ggagattttg attgcgtacg cagatcgtag
    10801 gccagataag gggtttacgc tgcatcaggt aacaaggcgc gccaaaaagc aaaacgcccg
    10861 catatgcggg cgttcaggaa cacgtcgtgg agggaagatt attcttcttc gtcgcgcagc
    10921 ggaacaatca gcatatcaac gtgaacggtg ttgatcagct gacgtgcgga agacatcagt
    10981 ttgctccaga agtcctggtg gtgaccacaa accaccagat ccatatcgta tttcttgatt
    11041 gcatcgacca gaacctggcc caggtcgccg ctgccgctca gggtttcagt gattgggtag
    11101 cctgcattag tggaaagctc ggtcagcgca tgatgtgtct cttcagagat gcgtttctgc
    11161 atatcaccca gattcacatc aataagcccg gtgtataggt cagagtagtt tacatctacg
    11221 tggatcagag aaactttcgc attgtagggg cgagccatag agactgcttt ctctaccaga
    11281 actttgcttt ccggggagag atcgaccgcg atgagaatgt gtttataagc catagtgtta
    11341 ctccttccat aaagttgtcg atgactggcc agctagcgtt tcttgtgccg ttacgtcacc
    11401 cgcgtcctgc gaactacgtg cgcggggcta acccagccgt aaagattcag tgttaagacc
    11461 atccttacct tatagcgacc cggatgatcc gtcaatccgc cttgcttacc cgttagataa
    11521 acaaattttc gacatgaata tattgatagt ggttaacctt ctggaaaaaa aacaacctga
    11581 tctcctacac tatctataga gccgctcgga tgttggtcac cacaaaagcg gtttctccag
    11641 gacctggtgt taactgattg gcagcgtatc gggagcggta gccccggggt ggaactccgc
    11701 gggcaggtcg ccggggagga ggtatgataa gcaccgtcgc attattttgg gctttatgtg
    11761 tcgtttgcat tgttaacatg gcgcgctatt tctcatcact acgcgcgttg ttagtggtac
    11821 tgcgtaactg cgatccattg ctctatcaat atgttgatgg agggggcttt tttacctcac
    11881 atggccaacc caacaaacag gtgcgtctcg tttggtatat ctatgcccaa cgttatcgcg
    11941 atcatcacga tgatgagttt attcgccgct gtgagcgggt gcgtcggcag tttattctga
    12001 ctagcgcatt gtgtggtctg gtggtggtca gcctgattgc attgatgatt tggcattaag
    12061 cacttcagca ataataacgc ataaaaaaag ctggccagta aactgacccg ctcgagactg
    12121 attacaaacg caaaattgcc tgatgcgcta cgcttatcag gcctgcataa tctctgcaat
    12181 ctattgaatt tgctgttttt gtaggccgaa taaggcgttc acgccgcatc cggcatgaac
    12241 aaagcgcact atgtcacaat ctgaagcggg ccagctgacc cgctcgtttt ggtgcgtacg
    12301 attacaggaa ctgcaaggag agccagtaca gcccgccgga aagcagtacc gcagccggaa
    12361 gggtaaacac ccaggccatc agaatgctgg tcacggtttt acgctgtaag ccgccaccat
    12421 ctaccaccat cgtccccgcg acagaagagg agagtacgtg agtagtggaa accggcatcc
    12481 cggtataact cgccaggccg atagacactg ccgccgtcat ctgggcagac atcccctgag
    12541 cgtaggtcat gcctttctta ccgattttct caccgatagt cgttgccaca cgacgccagc
    12601 caatcatcgt accgatacct aacgccagcg cgaccgccat gatgatccac accggtgcat
    12661 actcgatggt gctaagcatg tcggacttca gtttcttcaa caggcgctga tcgtcagcac
    12721 tcacgccagg tatcttcact actttgtcga tagtgtcaga aacgcacagc ataatgcggc
    12781 gcatctggct acgttgatca agcgacagct tgtcgtagct ttccacatcg gtagttaaca
    12841 tacctttcag gcggttgagc gcgttaatgg tattcgacgg atggcaatgg aactccgcgg
    12901 gttgcgttgc gccagcttcc ggagccggta ctaactgatc agcaccggta gcctgtttca
    12961 gcagcgcagg atgctgctca aagtaagctt cgacgttgtt gatggcatca cgggtacggg
    13021 tgatttcgta gccagtggca ttcatattca ccacgaagcc tgctggtgcg acaccaatca
    13081 ataccaacat aaccagacca atgcctttct gaccatcgtt cgcgccgtgc gaaaacgcca
    13141 cgccgatagc ggaaaggatc agcgcaatac gcgtccagaa cggcggcttt ttcttgccgt
    13201 ctttcttttc acgctccgct ggggtcaggt ggatacgggc gcgtttcttg gtgccgctcc
    13261 agtagcgacg cagcaagaaa atcagaccgc cagcaaacac caggccgaca ataggggaaa
    13321 caatcagaga accgaaaata cttaatactt tcgggatatt gagtgcatcc accactgacg
    13381 tcccggtcat caacgcattg gttaaaccaa tcccgatgat cgcgccaatc agtgtatgag
    13441 agctggatgc aggtaaacca aagtaccagg tacccaggtt ccagataatc gccgccagca
    13501 acatagagaa caccatggca aggccatgag acgatcccat attaagcagc agatccgtcg
    13561 gcagcatatg cacaatggca taggcaacac tcagaccacc cagcaaaaca cccaaaaagt
    13621 tgaacaccgc cgccataacc acggcgagct gagaacgcat cgcgcgggta tagataacgg
    13681 ttgccacggc gttggctgtg tcatggaaac cattgatggc ttcgtagaac agcacaaaag
    13741 ccagtgcaag caataataac agcccggtat gcaaatccag gccagcaaac aaatgtagca
    13801 taggacgtta cgccattttg aggacatgaa cgcggcgcat tatcagtgac tttcgcggcg
    13861 cgggcaaagt gaaatataga ctttttttga tttgcctcct gtatggattt cactcaaaaa
    13921 ataattatct tatataattc aggcaaatac ttccttttag taatattgat gctggtgtga
    13981 ccactgagga atctttacaa ttcacgcccg ttttttctaa gaggagcgca acgtggaaag
    14041 gtttgatgcc attattatag gcgctggtgc ggcgggtatg ttctgttctg cgctggcagg
    14101 tcaggcagga cgccgggttc tgctgatcga taatggtaaa aaaccagggc gcaaaatcct
    14161 tatgtctggc ggtgggcgct gcaactttac caacctttat gtcgaaccag gcgcttatct
    14221 gagccagaat ccgcattttt gtaagtctgc actcgcgcgt tttacccagt gggatttcat
    14281 tgatctggtc aataaacacg gcatcgcctg gcacgagaaa acgttaggac aactcttctg
    14341 cgatgactcc gcgcagcaga ttgtcgacat gctggtggat gagtgcgaga agggcaatgt
    14401 aaccttcaga ttgcgtagcg aagtgctgag tgtggcgaag gatgaaacag gcttcacgct
    14461 tgaactgaac ggcatgactg tcggttgcga aaagctggtc atcgcgaccg gtgggctgtc
    14521 aatgccgggg ctgggcgcat cgccgtttgg ttataagatt gccgaacaat ttggcctcaa
    14581 cgtgctgccg acccgtgcgg gtctggtgcc attcactctg cataaaccgt tgctcgaaga
    14641 gttacaggtg ctggcgggcg tggcggtgcc ttccgtgatt accgctgaaa acggcaccgt
    14701 tttccgtgag aacttactct tcacccaccg cggcttatct ggaccggcgg tgttgcagat
    14761 ttccagctac tggcaaccgg gtgaatttgt cagtatcaat ctgctaccgg atgtggacct
    14821 cgaaaccttc ctgaatgagc agcgtaacgc acatccgaat cagagcctga aaaacacact
    14881 ggcggttcat ctaccgaagc ggttggttga acgcttacag caactcgggc aaatcccgga
    14941 tgtttcgcta aaacagctca acgtgcgtga ccaacaggca ctgattagca cattgaccga
    15001 ctggcgcgta caacccaacg gcactgaagg ctatcgcact gccgaagtga cgctcggcgg
    15061 cgtggacacc aacgaactct cttcacggac gatggaagcg cgcaaagtgc ctgggctgta
    15121 cttcatcggc gaagtgatgg acgttaccgg ctggctgggg ggctataact tccagtgggc
    15181 gtggtcgagt gcgtgggctt gtgcgcagga tttgattgca gcgaagtcgt cctgacctgt
    15241 tacctaaaga tgaaaattat tagagccgca ttaaaaatga gctaattttg atagtggcta
    15301 tcttgtgatt attttctaat gagcccgtga actgaaaccc tccaggctta atataaggtg
    15361 gaaggaaaga tgattgaaaa ctcacccagt ggccagatct ttataaaata tgaacaggct
    15421 gaaaaagaca gtaactttgt tgttttttat ccttaaccac tatgcattaa tgctgcgtta
    15481 tttcatgatg cctaagaaaa accagcgtta cgcaaatggt caacgctggt tttatccggt
    15541 acgttgcaat tattttttag cagaacctgc ttctaatatt gaaacgattg agaacaacgt
    15601 aaagcatacc gctcccaaac cgaccaaaac ccgcgaagga ataaaatatc ccatgtcggt
    15661 ctgcgccatt tccgcaagaa acgatgccag gaaaaggcaa aacaggcagg tgaagacggg
    15721 aatcatcggt attctgttgg ctaacgaaca ggtacgtctc cataccagtg ccagtagcca
    15781 gacttttgag aatatgctgt aacagatcat tccgaggtaa ataagaataa tgccgggagc
    15841 cagtcgggcg cttgcatcgg aactgactaa gacgtatatc ccctgcagta ccgtgattga
    15901 gccgagaaaa ataacccaat aacaccacag ccagtgttct ttagttgaaa acgtattacg
    15961 tgtttgatgg acaattgtgg caacaaggcc aattagacag gcacagattg cggttaaccc
    16021 aagcaatacg tgacccgcga cataatgcgg agttatgtca gcactacgta acagcgtaat
    16081 agaccaaatg aaaccgagca gggtgagtaa aacgggaacg gcaatgaggc agttaccgat
    16141 taatgaagaa taagcctgta ccggtgttcc gtcgctcttg ctccccgctg catttttggg
    16201 aatgagcaga aagtgaccgg acgacgctgc caccgtcgat acacatgcgg caatcatacc
    16261 aatgccgaaa ataacatagc cggcgacaaa ctcgtctgcc atcacatcat tgcctgctaa
    16321 cagtgcccaa ccccatatca tggtgataat tgaccctgca tagccaataa tggggaacaa
    16381 tatattgtaa aacgtattaa cgccgcgcgt gagctgcgaa atgataataa atgcggtagt
    16441 gaataatgcc aggcatatgg ctgccagaga aatcagcaca tgacccgcga cgaaatactc
    16501 gctttgccct gaactttcca gcacaaagcc acctaacgcg atacaaatta agcccataat
    16561 caaagggatt aatttgaaaa gccacccgat atatatgttc actttaaact cctcaggt
//
LOCUS       DADLFA010000086        16569 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000086 DADLFA010000000
VERSION     DADLFA010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16569
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.086"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..2017
                     /gene="fhuE"
                     /locus_tag="JQH65_002349"
     CDS             <1..2017
                     /gene="fhuE"
                     /locus_tag="JQH65_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HAY6139739.1"
                     /translation="DYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLEISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAIDMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSGWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNMEGSIVYGAPRNFSITGTYQF"
     gene            complement(2077..3510)
                     /gene="ptsG"
                     /locus_tag="JQH65_002350"
     CDS             complement(2077..3510)
                     /gene="ptsG"
                     /locus_tag="JQH65_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HAY6139740.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMHDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(3805..4602)
                     /locus_tag="JQH65_002351"
     CDS             complement(3805..4602)
                     /locus_tag="JQH65_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY6139741.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(4613..5617)
                     /gene="holB"
                     /locus_tag="JQH65_002352"
     CDS             complement(4613..5617)
                     /gene="holB"
                     /locus_tag="JQH65_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAY6139742.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYTVTWLSREVTMSQDALLAALRLSAGSPSAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(5614..6255)
                     /locus_tag="JQH65_002353"
     CDS             complement(5614..6255)
                     /locus_tag="JQH65_002353"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAY6139743.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(6245..7267)
                     /gene="yceG"
                     /locus_tag="JQH65_002354"
     CDS             complement(6245..7267)
                     /gene="yceG"
                     /locus_tag="JQH65_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HAY6139744.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(7270..8079)
                     /gene="pabC"
                     /locus_tag="JQH65_002355"
     CDS             complement(7270..8079)
                     /gene="pabC"
                     /locus_tag="JQH65_002355"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HAY6139745.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(8199..9440)
                     /gene="fabF"
                     /locus_tag="JQH65_002356"
     CDS             complement(8199..9440)
                     /gene="fabF"
                     /locus_tag="JQH65_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAY6139746.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKAIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNLDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(9528..9764)
                     /gene="acpP"
                     /locus_tag="JQH65_002357"
     CDS             complement(9528..9764)
                     /gene="acpP"
                     /locus_tag="JQH65_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAY6139747.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(9975..10709)
                     /gene="fabG"
                     /locus_tag="JQH65_002358"
     CDS             complement(9975..10709)
                     /gene="fabG"
                     /locus_tag="JQH65_002358"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HAY6139748.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(10722..11651)
                     /gene="fabD"
                     /locus_tag="JQH65_002359"
     CDS             complement(10722..11651)
                     /gene="fabD"
                     /locus_tag="JQH65_002359"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAY6139749.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(11667..12620)
                     /locus_tag="JQH65_002360"
     CDS             complement(11667..12620)
                     /locus_tag="JQH65_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAY6139750.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVANQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(12688..13758)
                     /gene="plsX"
                     /locus_tag="JQH65_002361"
     CDS             complement(12688..13758)
                     /gene="plsX"
                     /locus_tag="JQH65_002361"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAY6139751.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLRPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(13839..14012)
                     /gene="rpmF"
                     /locus_tag="JQH65_002362"
     CDS             complement(13839..14012)
                     /gene="rpmF"
                     /locus_tag="JQH65_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAY6139752.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(14064..14585)
                     /gene="yceD"
                     /locus_tag="JQH65_002363"
     CDS             complement(14064..14585)
                     /gene="yceD"
                     /locus_tag="JQH65_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HAY6139753.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            14784..15368
                     /gene="yceF"
                     /locus_tag="JQH65_002364"
     CDS             14784..15368
                     /gene="yceF"
                     /locus_tag="JQH65_002364"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HAY6139754.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLCEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(15480..16439)
                     /gene="rluC"
                     /locus_tag="JQH65_002365"
     CDS             complement(15480..16439)
                     /gene="rluC"
                     /locus_tag="JQH65_002365"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HAY6139755.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRSQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
BASE COUNT         4102 a         4507 c         4000 g         3960 t
ORIGIN      
        1 tgattacagc gtaacgtcta cctctgcggg taccaaaatg cagatgactc aacgtgatat
       61 tcctcagtcg gtcactattg ttagccagca gcggatggaa gatcagcagt tacaaacgct
      121 gggcgaagtg atggaaaaca cgctggagat cagcaaaagt caggcggatt ccgatcgtgc
      181 tctttattat tcccgcggat tccagatcga taactatatg gttgatggta tccccaccta
      241 ttttgaatcg cgctggaatc tgggcgatgc actttctgat atggcactgt ttgaacgcgt
      301 agaagtagtg cgtggcgcga caggactcat gaccgggacg ggtaatccat ctgcggcaat
      361 tgatatggtt cgaaaacacg cgaccagtcg tgaatttaaa ggcgatgtct cggcggaata
      421 cggtagctgg aacaaagaac ggtatgtggc ggatttacaa agcccactca ccgaagacgg
      481 taaaatccgc gcgcgaattg tcggcggcta ccagaataac gactcatggc tggaccgcta
      541 caacagtgaa aagaccttct tctccggcat tgtcgatgct gatttaggcg atcttacgac
      601 gctttcagcc ggttacgaat atcagcgcat tgatgttaat agccctacct ggggcggttt
      661 accgcgctgg aatactgatg gcagcagcaa cagttacgat cgtgcacgca gtaccgcacc
      721 tgactgggcg tacaacgata aagagatcaa caaggtcttt atgaccctga agcagcggtt
      781 tgctgatacc tggcaagcga cactgaatgc cacccactct gaagtcgaat ttgacagcaa
      841 aatgatgtat gtcgatgcct atgtaaacaa agcggatggt atgctggttg ggccatacag
      901 taattatgga cctggctttg attatgtcgg cggcaccggt tggaacagtg gcaaacgtaa
      961 agttgatgcg ctggatttgt tcgctgacgg tagttatgaa ttgtttggtc gtcagcacaa
     1021 tctaatgttt ggtggcagtt acagcaaaca aaacaatcgt tacttcagtt catgggccaa
     1081 catcttcccg gatgaaattg gcagtttcta caactttaat ggcaatttcc cacaaaccga
     1141 ctggtcacca cagagcctgg cacaggacga taccacacat atgaaatcgt tatatgctgc
     1201 cactcgagtc acccttgccg atccgctgca tctgatcctc ggcgcacgtt ataccaactg
     1261 gcgggttgat acgctgactt acagcatgga gaaaaaccac accacgcctt acgctggtct
     1321 ggtgtttgac atcaatgaca actggtcgac ctacgccagc tatacctcta ttttccagcc
     1381 gcaaaatgat cgtgacagtt caggcaaata tctggctcca atcaccggta acaactacga
     1441 gctgggtctg aaatcgggct ggatgaatag ccgtctgacc accacgttag ccatcttccg
     1501 tattgagcag gataatgtcg ctcagtccac cggtacacct atccccggca gcaacggcga
     1561 aaccgcctat aaagcggtgg atgggacagt cagtaaaggg gtggaatttg aactcaacgg
     1621 cgcaattacc gacaactggc agctgacatt tggcgcaacg cgctatattg cagaggataa
     1681 cgaaggaaac gccgttaatc ctaatctgcc acgcaccacg gttaaaatgt tcaccagcta
     1741 tcggttgcct gtcatgccag agttgacagt cggcggtggt gtgaactggc aaaatcgcgt
     1801 gtataccgac accgtgacac cgtatggcac cttccgcgcc gagcaaggta gctacgcgct
     1861 ggtggatctc ttcacccgct accaggtgac gaaaaacttc tcgttacagg ggaacgtcaa
     1921 taacctgttc gacaaaacct acgataccaa catggaaggt tctatcgtct acggcgcacc
     1981 gcgtaatttc agcattaccg gcacgtatca attctgaata acacctgtaa aaaaggcagc
     2041 catctggctg ccttagtctc cccaacgtct tacggattag tggttacgga tgtactcatc
     2101 catctcggtt ttcaggttat cggatttagt accgaaaatc gcctgaacac cagaacccgc
     2161 aaccactacg cccgctgcgc ccagtttctt caggccagcc tgatccactt tagacacatc
     2221 agcaacgctg acgcgcagac gggtaataca tgcgtcgagg ttagtaatgt tttctttacc
     2281 accaaatgca gcaaccagag ccggtgccat ttcgctggta cctgttgctt ttgcatcttc
     2341 agtcgcgtct tcacgacccg gcgttttcag atccagtgct ttaatcagca cgcggaagat
     2401 ggtgtagtaa acaatcgcat aaccgatacc gacgatcggg aacagccaca gtttgctgct
     2461 gttaccagac agaacgataa agtcgatcag accgtgcgag aacgacgtac cgtcatgcat
     2521 ccccagaaga atacagattg ggaatgccag gcctgccaga atcgcgtgga tgatgtacag
     2581 gatcggcgca acgaacatga aggagaactc gatcggctcg gtgataccgg tcaggaacga
     2641 ggtcagcgcc gcggagatca taataccgcc cactttcgcg cggttttctg gtttagcaga
     2701 gtgccagata gcaattgcgg cagctggcag accgtacatt ttgaacagga agccaccaga
     2761 cagtttaccc gcagtcgggt cacccgccat ataacgcgga atgtcgccgt ggaaaacctg
     2821 acctgctgcg ttggtgtatt caccaatctg catctggaaa ggtacgttcc agatgtggtg
     2881 cagaccaaac ggtaccaggc aacgttcgat gaaaccgtaa atgccaaacg caactaccgg
     2941 gttctggtaa gcagcccact gagagaaggt ctggattgca gaaccaatcg gcggccaaat
     3001 gaaggacagc acaacgccag taaagatggc agccaggcca gaaatgatcg gcacaaagcg
     3061 tttaccggca aagaagccaa gatactcagg cagcttaata cggtagaaac ggttaaacat
     3121 gtacgctgcg atcgcaccgg agataatccc tccgagtacg ccagtatccg ccaggtgttt
     3181 agaggcgatt tcttcagcag gtaaatgcag taccagtggc gcaaccacgg ccatggtttt
     3241 aaccatgatg ccataggcaa caactgcggc cagcgcggat acgccatcgt tattggtaaa
     3301 gccgagggcg acaccgatcg caaaaatcag tggcatgttt gcaaagacgg aaccgcctgc
     3361 ttctgccata acatgcgata caacggcggg cagccagctg aaattcgcgg aaccgacgcc
     3421 cagcagaata cctgcgatag gtagtacgga taccggcagc atcagcgatt taccgacctt
     3481 ttgcaggtta gcaaatgcat tcttaaacat aattgagagt gctcctgagt atgggtgctt
     3541 tttttacgtt ctcacgcgtg gcaagggggg agagcctcgc cgtgtacagg gcatctaagc
     3601 gccctttatt tattacacag agtaaaataa ttcagtgcca atttgtttga cggctatcac
     3661 gtttcaactt cagatcattg gagaagttgc acattttgac aaaacatatg tcacaatatt
     3721 tcaaacgcgg ttattcaccg cccaattttc ggcggtgaac tttactcgtt ttagccatta
     3781 attacgagct ttaaaaaaac tcattcaacg gatggattga aggcgggaag cgtcgatgtg
     3841 aaacagacgg gcgaagttat cggtggttac ctgcgccagt tcttcaacgg caacaccttt
     3901 caacacagcc atgtattctg caacgtcacg aaccatcgca ggttgattct cttttcctcg
     3961 atgcggtacc ggcgcaaggt aaggtgagtc agtttccacc agtaatcgat ccagggggac
     4021 ataacgcgca gcatcgcgca gttgctccgc attacggaag gtcacaatgc cggaaaagga
     4081 gatgtaaaat ccgagatcca gtaatttacc cgccgtttct ctgtcctctg taaaacagtg
     4141 tagtacgcca ccgcaatccg tcactttttc ttcgcgcaga attgccagcg tatcggcacg
     4201 ggcgtcacgg gtatggacga taaccggctt gttcagttca cgaccaatct ggatatgatg
     4261 gatgaaggac tcttgctgac gtactttagt ttccggtgtg taataataat ccagcccggt
     4321 ttcacccagc gctacaacac cctcttctgc cgccagacgg cgtaaatctt ctacatcgta
     4381 gggatcattc tggttgagtg gatgcacgcc acaagaaaat acgacgttgt cacgttcgcc
     4441 taccagatcc cgcatatgta aataacccgg taatgttgtg gcgactgcca gacaaaattt
     4501 cacatcgcgt gcggcggctt tcgccagaac gtcatccacg tccttatgca aagattcata
     4561 atccagacca tcgagatggc agtgtgagtc gactaaaaac atgatgtctc tcttaaagat
     4621 gaggaaccgg tagcacaacg cccggttgca ggtaatgctc aatacgcagt aaaagatcgg
     4681 tgatgagaag ctcgcggttg atgcctgtga cagacattaa ctgttcacga atgtggcaaa
     4741 catcccccag tatagcctgc aggcgcgagg gagaaagatg gtttgccagt tcggcgacca
     4801 ggcccggcac atcaacattg gtcacctgtg cagcgccatg atggcgtttt agcgcatcca
     4861 tcagcaacgt tgccagccag tgtaaacgcg ccggagcttg ttcatgatta agggccgcca
     4921 gcagcgaata ccagtcgccc gatggtacgc tatatgccaa cgcctgacac aatgtttcac
     4981 gagcctgcca gttatctccc tgaaacaacg ccagtgccgc gctaggcgaa ccggcactta
     5041 agcgcaatgc ggcaagtaat gcatcctgtg acattgtcac ttcgcgtgaa agccaggtca
     5101 cggtgtactg ttccggcggc ggcgcaaggt aatgtaaccg acaacgacta cgtaatgttg
     5161 ccagtaaacg ttcaggctcg cgggtagcca ggaaaaacca ggtttctgct ggtggctctt
     5221 caagcgtttt cagcaatgcg ttagccgcgg cgtcggtcag taaggcagca tcggttaccc
     5281 agacaacttt cgcaccacct aggcgtgcgt gctcattcag cttttcggtg acctcacgta
     5341 ccgcatcaat gcccagcgta tttttccctt tttcgggagc cagggtgtag taatcgggat
     5401 gcgtgccagc ctgcatcaac tgacatccac gacagtgacc gcaacttttg tggccctgcg
     5461 gttgttggca gagtaaataa cggctcaggg cgtagattaa agcatcatcg cccatgcccg
     5521 gtaacgcctg aatgagtagc gcatggtgac ctcttccggc ctgatagctg gctaccagtt
     5581 tttcgaaatc aggtcgtaac catggatacc atctcatgcg tccaactcct tcacccagtg
     5641 ggtcacggta gtacggattg catccatcac ggcctccagc ggctgggtgg catcaatggt
     5701 atgaatactt ttatcttgtg cagccagttc cagatagcgg gcgcgggtgc gattaaagaa
     5761 atcgaaagac tcttgctcaa tacgatccag ctcgccgcgc gcacgcgcgc gttttaagcc
     5821 aacttccggg gtaacatcga gatagagcgt taagtcaggg cgaaaatccc cgagaacagc
     5881 atcacgcagt gttgccagca tatgttggtc aataccacgt cctccgccct gatacgcctg
     5941 agtggagaga tcgtggcgat cgccaatcac ccaggtgccg ttagccagag ctggtttgat
     6001 gaccgtttct accagttgaa cgcgcgcggc ataaaacatc agaacttcgg ctttatcggt
     6061 aatgacttca tcgcctaccg atttgatatc cagcaccagg cttcttaact tttcggcaag
     6121 ttgcgtaccg ccaggttccc gagtgaaaac catgtcgcgg atacccagtt gctcaagcgt
     6181 ctcaaccacc acattacgcg cggtagtttt gcctgcgcct tccagcccct caatgacgat
     6241 atacttactg cgcatttttt tccttaagca ctttcagata atcctgcaca gacttgttat
     6301 ggctggcaag attggtatta aacgtgtgac cacctttacc atcggccaca aaatagagat
     6361 acggcgtttt tgccggatgc gcagcagcct tcagcgaatc cgcccccggc gtagctatcg
     6421 cacctggcgg caaaccggta atggtatagg tgttatacgc tgtcggcgtt tccaggtctg
     6481 cacgagaaag tttgccatta taacgctctc ccatcccgta aatcacggtt gggtcggtct
     6541 gcaggcgcat accaatgcgt aaacggttga taaataccga ggcaacctga tcgcgttcac
     6601 tggcaacggc ggtttctttt tcgataattg atgccatcgt caccagctgg tttttatctt
     6661 tataaggcag accgtccgca cgcccttccc aggcgctatc gaccgctttc accattttct
     6721 tgtgcgctcg cttgagtaac gcgacatcgg tggtattggc ggtatacatc caggtgtctg
     6781 gccagaacca accttcaatc cactccgggt tttccagttc aagtgcctgc gctacggtgg
     6841 cgtacttatc gtcactcagc gtgtgcttga tatacggagc ctcacgcaat tgcttgaggt
     6901 agtcgctcag acgcatccct tctaccagtc gcagcgggaa ctgtgcttct ttaccgcttt
     6961 ccagcaattt cagcatctcg cgcacggtca tctgcggtgt aaagcggtaa gtcccggctt
     7021 taaagtgaga aagatccggt tcgatacgca gcaaccattg aaaaacccgt gggcgattga
     7081 tgatcttatc ggcataaagc tgttcaccaa gcgccagacg tccggtccct ggcttcaggg
     7141 taaatatcgt ctcttctttg ataagcaatt tgctgtcggc aagatggcga accttccaga
     7201 cgcccacacc agcggcgata cccagtacca ccagcaataa caagataatc aataacactt
     7261 ttttcatgac taattcgggc gctcacaaag tggggctaaa tattcatata acgttgctga
     7321 cgaaaaggag acatcgccac aggcacgtac gggcatcact ggcattaacg cattacaaat
     7381 aaccatctca tctgcctgca acgcctcttc cagagaggct tgcacttcga caagctgata
     7441 agaggattgt gccagcaaac ggatacagaa ttgtcgcata atgccgttaa cacctgcctg
     7501 atccagtcgc ggcgtataaa ctacattgcc cttccgccag aacaaattag ccgcacagca
     7561 ttccgtaacc cacccttcgc tgtcaaggac cagcgcctca tcagcgtttg tctgctcaag
     7621 atgagagcgg atcaatactt gctcaagccg attaagatgt ttaatacctg caagatgggg
     7681 attgcgcccc agccgcaccg ggcttagcgc caacgtcatc ccttcgttac gcaaacggtc
     7741 gtaatgtgca ggataaaccg taacggagag aatccgcgtt gccggtccac tgttcaatgt
     7801 gctgtaccct cgcccgccac taccgcgact gatcacgact ttcagcacac cattttgctg
     7861 ttctgctgcc agcgttttca tctcttgttc aagctgaggc cagaagtcac agaaaatcat
     7921 caaccgctga caagcatcct gtagtcgctg gatatgcgcc gataacaaac tgactttacc
     7981 gtcgataact ctggcggtgg taaaacaacc atcaccaaac tgcgttgccc gatcgcttac
     8041 tgccagcgat tcctgcttat gaccgttaat taagaacata ccggctcctt atcagagaat
     8101 tgttagtgtg gcagcatgct cactacggaa caagtcggaa taaaagctaa gaaaaaaggc
     8161 ccgcaagcgg accttttata tgggtgggaa atgacaactt agatcttttt aaagatcaaa
     8221 gaaccattag tgccaccgaa gccgaaggag ttacacagcg tgtattccat tccgctgacc
     8281 tgacgtgctt catgcggtac gaaatccaga tcgcaacctt catccaggtt atccaggttg
     8341 atggttggcg gaacagcctg atcgcgcagc gccaggatgg agtagataga ctctactgcc
     8401 cccgccgcac ctaacaggtg accggtcata gatttcgtgg agcttaccaa cacacggctt
     8461 gcagcttcac cgaagatggc tttcaccgcc tgcgcttcag ctttatcgcc agccggcgta
     8521 gaagtaccgt gcgcgttaac gtagccaatc tgactcgctt caatgcctgc atcacgcaga
     8581 gcatttgcca ttgccagagc tgcgcctgcg ccattttctg gcggtgacgt catatgataa
     8641 gcatcgctgc tcataccaaa gccgacgagt tcagcgtaaa ttttcgcacc gcgttttttc
     8701 gcgtgttcgt actcttctag taccagcata ccggcaccat cgcccagtac gaagccatca
     8761 cgctctttat cccacgggcg gctcgccgct tgcgggttat cattgcgggt agataatgca
     8821 cgtgccgcgc caaaaccacc aacgcccagc ggcgtactgg ctttctctgc gccacctgca
     8881 accatcacgt cagcatcgcc atacgcgata atacgcgcag catgaccaat gttgtgcacg
     8941 ccggaagtac aggcggtcgc gatagagatg cttgggccac gcaggccata catgatagtc
     9001 agatgacctg ccaccatgtt cacaatcgtt gacggaacga agaatgggct gatcttacgt
     9061 ggaccaccgt tcatcagaga tgtgtggttt tcttcgatca gtccgaggcc gccaatcccg
     9121 gagccaattg cggcaccaat gcgggttgcg ttctcttccg ttatttcaag gccagaatcc
     9181 tgcatggcct gaacgccagc gacaattcca tattgaatga aggcatccat cttgcgctgt
     9241 tctttgcgcg agataatgtc ctcacagtta aaatccttta ctaagccagc aaatttcgtt
     9301 gcataggcgc tagtatcgaa atggtcgatt aggctgatgc cactctgacc ggcaagcaga
     9361 gctttccagg tagactctac ggtattgccg acaggagaca acatgcccag tccggtcaca
     9421 actacacgac gcttagacac gtttgtcctc cagggaggga aaaaatgatt ctagtgggac
     9481 aaaaagataa aactcaggcg gtcgaacgac cgcctggaga tgttcactta cgcctggtgg
     9541 ccgttgatgt aatcaatggc agcctgaacg gtggtgattt tctcagcttc ttcgtccgga
     9601 atctcagtat caaactcttc ttccagagcc attaccagct caacggtgtc aagagaatcc
     9661 gcgcccaggt cttcaacgaa agaagcattg ttggtaactt cttcctgctt aacgcccagc
     9721 tgttcgccga taattttctt aacgcgttct tcgatagtgc tcatactctt aaatttccta
     9781 tcaaaactcg ctttcgcgat ggttttcgta gtgtataaaa tgttgaaaaa tttgcaacta
     9841 aatcccggca ggtcttacca cgattttacg ttattttgag gccttacccc ccaataacgc
     9901 aaatcatttt gcactaatta ttactcataa ccacgcagac tgcgcgcaac atgagcattt
     9961 tgtgcaaatc gcggtcagac catgtacatc ccgccgttca catgcaaagt ttcacccgtg
    10021 atgtaagctg cttcgtcgga tgccaggaat gcaaccgcgt tggcgatttc ctgtgcgccg
    10081 ccgaggcgac ccgcaggaac ctgcgccagg atacccgcac gctggtcatc gctcagcgca
    10141 cgtgtcatgt ccgtttcaat aaagcccgga gcaacaacgt ttacagtaat accgcgtgac
    10201 gcaacttcgc gcgccagtga tttactgaag ccgatcaagc ccgctttcgc cgcagcgtag
    10261 ttggcctgac cgccatttcc catggtacca accacagaac cgatagtgat aatacgacca
    10321 tgacgctttt tcatcatagc gcgcattacc gcttttgaca gacggaaaac agatgaaagg
    10381 ttggtttcga taatatcgtt ccactcttca tctttcattc gcattaacag gttatcacga
    10441 gtgataccgg cattattgac caggatatcc acttcaccaa attctgcgcg aattttttcc
    10501 agaacagatt cgatagatgc cgggtcggtc acattcaaca tcagaccttt gccgttagca
    10561 cctaaataat cactgatcgc ctgagcgcca ttttcactgg tcgcagtgcc aataactttc
    10621 gcgccacggg ctgcgagcgt ttcagcaatt gcgcggccaa ttccgcggct tgcaccggtt
    10681 accagtgcga tttttccttc aaaattcatg attttcctct tttaaagctc gagcgccgct
    10741 gccatcgctg aaggttcgtt cagcgccgag gcggtcaggg tgtcgacaat gcgtttcgtc
    10801 aggccagtta gcactttgcc cgggccgact tcatagagat gttctacgcc ttgcgctgcc
    10861 atgtactcaa cagacttcgt ccactgaacc gggttataca actgacgtac cagtgcgtca
    10921 cgaatggcat caccattggt ttcgcatttc acatcaacgt tattcacaac aggaactgtt
    10981 ggtgcgttaa aggtgatttt cgctaattct actgccagtt tgtcggctgc tggtttcatc
    11041 agcgcacagt gagacggtac gctcactggt aacggcagcg cgcgttttgc gcccgcagct
    11101 ttacaggcag cgccagcacg ctcaaccgcc tctttatgac cggcaataac cacctgtccc
    11161 ggagagttaa agtttaccgg agaaacgacc tgaccttctg cagcttcttc acacgctttc
    11221 gcaatagacg catcatccag accgatgatt gccgccatag cgcccgtgcc ttctggtacg
    11281 gcttcttgca tgaacttgcc gcgcatctca accagacgca ccgcatcagc gaaatcaatc
    11341 acgccagcgc aaaccaacgc ggagtattcc cccaggctgt gaccggccat cattgccggt
    11401 gctttaccgc cctgctgctg ccatacgcga tacagcgcaa cagatgcagt caacagcgct
    11461 ggctgggttt gccaggtttt attcagttct tcagctggcc cctgctgggt cagcgcccac
    11521 aggtcgtagc ccagcgccgc agaagcttca gcaaacgttt cttcgacaat tggatagctc
    11581 gccgccatat cagccagcat tccaacggtt tgagaaccct gtccagggaa cacaaatgca
    11641 aattgcgtca tgttttaatc cttatcctag aaacgaacca gcgcggagcc ccaggtgaat
    11701 ccaccgccaa aggcttcaag cagaaccaac tgccccggct taatgcgccc gtcgcgtaca
    11761 gcttcatcca gcgcgcacgg gacagaggcc gcagaggtat taccgtggcg atccaacgtc
    11821 accacgacat tgtccataga cataccgagt tttttcgccg ttgcactgat aatacgcagg
    11881 ttagcctgat gcggaaccag ccagtccagt tgagaacggt caagattatt cgccgccagc
    11941 gtctcatcaa cgatgtgcgc cagttccgtt accgcaacct tgaagacttc gttgcccgcc
    12001 atcgtcagat gaattgaatt ctctggattc acgcggtcgg cgtttggcag cgtcagcaat
    12061 tcaccataac taccgtcggc atgcagatgg gtggagatga ttcccggctc ttcagaggca
    12121 gccagcaccg cagcgcccgc gccatcgcca aaaataataa tagtcccacg atctgttgga
    12181 tcgcaggtgc gcgccagtac atcggaaccg acgaccagag catacttcac cgccccagat
    12241 ttcacgtatt gattggctac gcttaatgca taggtgaaac ctgcgcaggc tgctgcaacg
    12301 tcaaatgccg ggcaaccttt aatgcccaac atgctttgaa tctgacaagc tgcgctcggg
    12361 aaagcgtgcg tagcagaagt cgttgccaca acgatcaggc caatctggtc tttctcaatg
    12421 cccgccatct caattgcgcg tgtcgccgct tcaaagccca tggttgaaac ggtttcgttt
    12481 ggcgcggcaa tgtggcgttc gcggataccg gtacgagtga caatccactc gtcagaggtg
    12541 tccaccattt tttccaaatc ggcgtttgtc cgcacttgtt cgggcagata gctgccagta
    12601 ccaataatct tcgtatacat gtacgctcag tcacttttcg gttatatacc gtcacttgca
    12661 aactgcgagt tcgctggcag cgtcctgcta ccgcagagtt ccgcttttgc caccgtccag
    12721 cagctcaaaa ccagctgggt atacagattc caggcgagcg gcaattcgct gaggaacttg
    12781 tcgctgcacc gcctgcactg cctgttcaat cgcgaccgca aaagctcgct gattggctgc
    12841 accatgactt tttatcaccg tgccgcgcaa tcctaacaga caggcgccgt tatactggtc
    12901 ggggttgagg tgactgaatc gcctcgtcag gctcttttgt agccaacgct ttaataacag
    12961 tagccaccac gaccgttttt tcccttcacc ctgagatttc agcagagaaa ggaacatcct
    13021 gacaacacct tccatcgtct ttaacgtgac atttcctgta aagccgtcac aaaccagcac
    13081 atctgtcttg ccagttaaca actcattggc ttcaagatag ccgatataat tgatagaagg
    13141 gattgtttta agcaccgctg aggcatcccg aatactgtcg agacccttta cttcttcttc
    13201 accaatattg agcaacgcca cgcgaggatt gggaatttcc accacctctt cagccagaac
    13261 tgagcccata atggcaaatt gcaccaacat tgtgctgtca caatcgacgt tggcccctaa
    13321 atcgaggacc accgttttgc ccttttgctg atgtggtaat accgtcacca gcgccggacg
    13381 ctcaatcccc tccaggggcc tgagtaataa ttttgccagc cccatcagtg ccccggtatt
    13441 accggcactg acacaggctt gcgctcgacc ttctttcacc agctccaggg ccatacgcat
    13501 tgaactccca cgactagcgc ggatagcttg cgaaggccgg gcatcactgg cgataactga
    13561 ctgcgcagga ataatctgca gacgcgaacg ttgttcaaag tcagctttag caagtaatgg
    13621 cgtgatggcg tcgggattgc cgactaaaag aagagtgagt tgcgaattag aattcagtgc
    13681 ctgcaatgct gcaggcactg tcacggaagg gccaaaatcc cctcccatga catctaacgc
    13741 cagggttaga cgtgtcaagg tatcgtcgcc gcccggtttg gtctttcccc agttgcctgg
    13801 ggaaaatcct cactaagctt catcacgcag acgcgtgatt acttagcgat gaccttgcgg
    13861 ccgcggtagt aaccgtcggc agtgatatgg tgacgcaggt gtttttcacc agaagttttg
    13921 tctacagaca ggctggtgac tgcggtcagc gcgtcatggg aacgacgcat gccacgtttg
    13981 gaacgggttg gtttattctg ttgtacggcc atggacctta ctcctcaatt acggtttatc
    14041 cccgatccaa cggggagcac caattactta cgctttaagc tggctaatac ggcaaatggg
    14101 tttggctttt gcacttcttc aggcagttca ccaaagacca tgtccgcttc ggacacttca
    14161 cagtgttcag aatcatgcac cggaactacc ggcaaggcga ggatgatttc atcttcaacc
    14221 attgcaagca gatcgatttc accgaattcg ttaacctcaa tcggttcata cgcttccggc
    14281 agtgcttcag cctgttcgtc tgaacgcaca ggactaaaac aatacgttgt gtagacctga
    14341 tgagtaaacg gcttcccgca acgctgacac tcgagcgtta ccgtcacctt cgcatcgccg
    14401 tttaacactg cgagacgttg gttatcgata gcgaacgaca tggagcattc cacatcactg
    14461 tccacactga ctacggattc ggcgacgcgc tcaacctgat caggggtata gataccctgg
    14521 taatcaaggc gtttttgagc cgtacgaacc ggatcgagag tcaggggtaa ttttaccttt
    14581 tgcatagggc gcgcatatta actttgtaac gtcatagagt caaagaaaaa ggcagcctgt
    14641 ggttgccttt tgccaataat tcgcacacat tgcgggttac tgctttattt tcgttcagtg
    14701 ctgataacga acatcaacct gcaaaacgaa gcgcatagtt taaaatggct atcatcaata
    14761 cgctatatct gatggaaaaa aatatgccta aacttatttt agcctccacc tcgccatggc
    14821 gtcgcgctct tctggaaaaa ctgcaaatct cttttgaatg tgcagcacca gaggtcgacg
    14881 aaaccccacg cagcgacgaa tcaccgcgac agttggtgct tcgactggca caagaaaaag
    14941 cgcaatctct ggcgtcacgt tatccggatc atttaattat tggttcagac caggtatgtg
    15001 ttcttgatgg cgaaatcacc ggtaaaccgt taacggaaga aaatgcccgt ctgcaattac
    15061 gcaaagccag cggcaatatc gtcaccttct atactggact ggcgttgttt aattcggcga
    15121 atgggcatct acaaacagaa gtggagcctt ttgacgtcca tttccgtcac ctgtgcgagg
    15181 cggagattga taattacgtg cgtaaggagc atcccctgca ctgcgcgggt agctttaaga
    15241 gtgaaggatt tggcattacg ctgtttgagc gtttagaggg acgtgaccct aacacgctgg
    15301 ttggtttacc gctaatcgcg ctgtgtcaga tgttacgtcg ggaagggaaa aacccgctga
    15361 tgggatagtt gtttgcgccg gatgcggtta cgtcgcatcc ggcaatcggt gcaggcggcc
    15421 tgatgcggcg taaacgcctt atcaggccaa ccgcccatta tcaggcttat atgcttgtat
    15481 tagcgcgcgt tacgcagctt ttgcaaacaa cgcttcaaac cttcatccat cggcgcttcg
    15541 atacgcatca cctcaccggt ccccggatgg gtaaacttca acgctgcggc gtgcaggaac
    15601 agacgattta atcccgtgcc tgcttcagtg agctgtctgt caaattcacg gtcaccgtag
    15661 cgatcgtcaa aggcaatcgg atgacccgca tactgtgtat gcacacggat ctgatgagta
    15721 cgtccggtta ccgggctaca acgcaccagg gtggcaaatg catagcgttc ttccacttta
    15781 aagcgtgttt ccgacggttt gccttcctga ctgacacgca cgatacgttc gccgctttgc
    15841 agaatatttt tcaataacgg cgcttgaacg ctcttcacat gcgactgcca ctgactgcgc
    15901 accagcgcca ggtaatcttt ttgcattcct ttttcacgta attgctcatg cagagaacgc
    15961 aacgccgagc gttttttcgc taccagcaac acacctgaag tgtcccggtc gagacgatga
    16021 accagttcaa ggaaccgcgc ttccgggcgc aacgcccgca aaccttcgat aacgccgaag
    16081 cttaaaccac tgccgccatg taccgccgta ccggaaggtt tattcagcac caggatatga
    16141 tcatcttcat ataagatgac gtccgccagc gccgccacct tttgcagatg tggcgaaacc
    16201 gcctcttctt cccgctcagc aacgcgaacc ggtgggatac gcacctcatc acccgcttcg
    16261 agtttatatt caggcttaat acgttttttg ttcacccgca cttcgccttt acgcaaaata
    16321 cggtaaatca tactttttgg tacgcctttc aattgggtac gcaaaaagtt gtcgatacgt
    16381 tgccccgctt cgtcagcggt gatagcaaca atttttacgg atggagtctc tgttttcatg
    16441 gtgggcgatt ctaattagcc aacaggattc gcgccactca tttttctatg cttatattta
    16501 gttttacacc ttattacttc actacgtgat cactttattg atggttatta aaccaatcac
    16561 cagcaagaa
//
LOCUS       DADLFA010000087        16538 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000087 DADLFA010000000
VERSION     DADLFA010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16538
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.087"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(45..190)
                     /locus_tag="JQH65_002366"
                     /pseudo
     CDS             complement(45..190)
                     /locus_tag="JQH65_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001626279.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
     gene            complement(299..393)
                     /locus_tag="JQH65_002367"
     tRNA            complement(299..393)
                     /locus_tag="JQH65_002367"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(357..359),aa:Sec,seq:tca)
     gene            686..2068
                     /locus_tag="JQH65_002368"
     CDS             686..2068
                     /locus_tag="JQH65_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HAY6139756.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVLGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            2078..4369
                     /gene="yicI"
                     /locus_tag="JQH65_002369"
     CDS             2078..4369
                     /gene="yicI"
                     /locus_tag="JQH65_002369"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HAY6139757.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGQDTNNQRNYMFERLDLGVGETVYGLGERF
                     TALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQSVSFEVGSEKVSK
                     VQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTSFTTNYDEAT
                     VNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRRLKAKGLKIC
                     VWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNPDACKWYADK
                     LKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAHIYNELVWNVLKDTVGEE
                     EAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFWSHYIGGFEN
                     TAPAHVYKCWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLKCRMMPYLYR
                     EAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAGDVQFYLPEG
                     RWTHLWHNDELDGRCWHKQQHSFLSLPIYVRDNTLLALGNNDQRPDYAWHEGTAFHLF
                     NLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRNVVKVNGLQD
                     GSQAESEQGLVVKPQGNALTITL"
     gene            complement(4422..6131)
                     /locus_tag="JQH65_002370"
     CDS             complement(4422..6131)
                     /locus_tag="JQH65_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HAY6139758.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPKAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDAHLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLLMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTTLDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGIVSSGEVTSAE
                     LVQ"
     gene            complement(6252..7643)
                     /gene="xanP"
                     /locus_tag="JQH65_002371"
     CDS             complement(6252..7643)
                     /gene="xanP"
                     /locus_tag="JQH65_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HAY6139759.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKTWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITSTSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            7923..9128
                     /gene="gltS"
                     /locus_tag="JQH65_002372"
     CDS             7923..9128
                     /gene="gltS"
                     /locus_tag="JQH65_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HAY6139760.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            complement(9162..11243)
                     /gene="recG"
                     /locus_tag="JQH65_002373"
     CDS             complement(9162..11243)
                     /gene="recG"
                     /locus_tag="JQH65_002373"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAY6139761.1"
                     /translation="MTGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(11249..11938)
                     /gene="trmH"
                     /locus_tag="JQH65_002374"
     CDS             complement(11249..11938)
                     /gene="trmH"
                     /locus_tag="JQH65_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HAY6139762.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(11945..14053)
                     /gene="spoT"
                     /locus_tag="JQH65_002375"
     CDS             complement(11945..14053)
                     /gene="spoT"
                     /locus_tag="JQH65_002375"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HAY6139763.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(14072..14347)
                     /gene="rpoZ"
                     /locus_tag="JQH65_002376"
     CDS             complement(14072..14347)
                     /gene="rpoZ"
                     /locus_tag="JQH65_002376"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAY6139764.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(14402..15025)
                     /gene="gmk"
                     /locus_tag="JQH65_002377"
     CDS             complement(14402..15025)
                     /gene="gmk"
                     /locus_tag="JQH65_002377"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAY6139765.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     DEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            15283..>16538
                     /gene="ligB"
                     /locus_tag="JQH65_002378"
     CDS             15283..>16538
                     /gene="ligB"
                     /locus_tag="JQH65_002378"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HAY6139766.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEIFRQQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMSDRLKELATAGFTLTQTYTRAVKNADEVARVRNEWWKAELPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     SVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLV"
BASE COUNT         3755 a         4226 c         4496 g         4061 t
ORIGIN      
        1 agtcgtataa aaaagggtgt tatggagcgt gtaaaacaac atcttcacct taatggcgat
       61 acagttggct gagagccagc agggggcaaa tcacctgcaa ataaacacgg cacatgctgg
      121 cgcgcatatc gcggatgcgt tcgaggacag aagaaccgac aggcggattc ttcagccgct
      181 catcgtccat cacaaaacct tagaccaggt attcctgaag ccgattgaat attgagcagt
      241 agaaaatatc tggattgaca gtattacaag gggttggaga acgtcagaag aggcattttg
      301 gcggaagatc acaggagtcg aacctgtccg ggaccgctgg cggccccaac tggatttgaa
      361 gtccagccgc ctcaccggag acgacgatct tccgcgcctc gattgctaca tggaggcggg
      421 gcgcattata gctacttcct tgagtttcta catcccccag atcagttttt tccgcctaat
      481 acacgcagcg tattatcacg aaagcattat agttcatata aaactcatat tttacaatga
      541 catatgagcg gaattaacgc taccacgatc acataaaaca agaatcataa attaataacc
      601 agatatcgga atattcgctc tcacagggat ggtttacaaa atgcgttatc ggtctcagca
      661 cccctatagc atcaaggaaa agcagatgaa gagtgaagtg ttgtccgtta aagagaaaat
      721 tggttatggc atgggagacg ccgccagcca cattattttc gataacgtaa tgttatatat
      781 gatgttcttt tataccgata tttttggcat tcctgccggt tttgtcggaa ccatgttttt
      841 ggtcgctcgt gcactggatg cgatttccga tccttgcatg gggttgttgg ccgatcgaac
      901 gcgctctcgc tggggtaaat ttcgtccgtg ggtactgttt ggcgcactgc cattcgggat
      961 cgtctgtgta ctggcctata gcacgccaga tctcagtatg aacggcaaaa tgatctatgc
     1021 agcaattact tacaccctac ttaccttact ttataccgtc gtcaatatcc cttactgcgc
     1081 attgggtggt gtaatcacca atgacccgac tcagcgtatc tcgctgcaat cctggcgttt
     1141 tgtgctggcg accgcgggag gcatgctttc tactgttctg atgatgccac tggttaattt
     1201 aattggcggt gataataaac cactcggttt ccagggcggt atcgcggtcc tttccgtggt
     1261 ggcattcatg atgctggcat tttgtttctt caccactaaa gaacgcgttg aagcaccacc
     1321 tacaacaacg tctatgcggg aagatttacg tgatatctgg caaaacgacc agtggcggat
     1381 tgtcggttta ctaaccattt tcaatatcct ggcggtgtgc gtactcggtg gggcgatgat
     1441 gtattacgtc acatggattt tgggcacgcc ggaagtgttt gtcgcttttc tcaccactta
     1501 ttgcgtgggt aacctgattg gttccgcact ggcaaaacct ctgaccgact ggaaatgtaa
     1561 agtcactatc ttctggtgga cgaacgccct gctggcagtg attagcctcg cgatgttctt
     1621 tgttcccatg caggccagca tcactatgtt tgtcttcatc ttcgtgattg gtgtgttgca
     1681 tcaactggtg acacctatcc agtgggtaat gatgtccgat accgtcgact acggcgagtg
     1741 gtgcaatggt aaacgcctga ccgggatcag ttttgctggc acgctgtttg tgctcaaact
     1801 ggggttggcc ttcggcggcg ctcttatcgg ctggatgctg gcttatggcg gatatgatgc
     1861 ggcagaaaaa gcgcagaaca gcgccacgat tagcatcatt attgcgctat tcacgattgt
     1921 tccggcgatc tgttatttgc tgagcgcgat tatcgctaaa cgctactact cactcacgac
     1981 gcacaatctg aaaaccgtta tggaacagct ggctcagggt aaacgccgtt gccagcaaca
     2041 attcacctct caagaagtgc agaactaagg aacggcaatg aaaattagcg atggaaactg
     2101 gttgattcaa cctggcctca atttgattca cccgcttcag gtgttcgagg ttgaacagca
     2161 ggataatgaa atggtggtct atgctgcccc ccgtgatgtg cgtgaacgta cctggcagct
     2221 tgatacgcct ttatttacgc tgcgcttttt ctccccacag gaaggtattg tcggtgtgcg
     2281 gattgagcat tttcagggag cgctgaataa cggtcctcat tatccgctca atattttgca
     2341 ggacgtgaag gtcacaatcg aaaacacaga acgttatgct gagtttaaaa gtggcaactt
     2401 aagcgcgcgt gtcagcaaag gtgagttctg gtcactcgat tttctgcgca acggtgaacg
     2461 tattaccggt caggacacga ataatcaacg aaattatatg tttgagcggc ttgatcttgg
     2521 cgttggcgaa acagtttatg gtctgggaga gcgttttact gccctggtgc gcaatggtca
     2581 gacggtagag acctggaacc gggacggcgg cacaagtact gaacaggcgt ataaaaatat
     2641 cccgttctat atgactaacc gtggctatgg ggtgctggtc aatcaccccc agagtgtctc
     2701 ttttgaagtg ggatcggaga aagtctccaa agtgcagttc agcgttgaga gcgaatatct
     2761 cgaatacttt gttatcgacg gtccgacgcc gaaagcggta cttgatcgtt atacccgttt
     2821 tactggtcgt ccggcgctgc cgcccgcatg gtccttcggc ctgtggctaa ccacttcatt
     2881 taccaccaac tacgacgaag cgacggtaaa cagctttatc gatggtatgg cggaacgcaa
     2941 tctgccgctg catgttttcc actttgactg tttctggatg aaagccttcc agtggtgcga
     3001 ttttgagtgg gacccgctga ctttcccgga cccggaaggg atgatccgcc gcctgaaagc
     3061 gaaagggctg aaaatctgcg tctggattaa cccctatatc ggtcaaaaat cccccgtctt
     3121 taaagagtta caagagaaag gctatttact caaacgcccg gacggttcgc tatggcagtg
     3181 ggataaatgg cagccaggtc tggcgattta tgactttacc aatccggatg cctgcaaatg
     3241 gtacgccgac aaactgaaag gtctggtcgc gatgggcgtt gattgcttta agaccgactt
     3301 tggcgaacgt atcccaactg atgttcagtg gtttgacggt tccgatccgc agaaaatgca
     3361 taaccattat gcgcacatct acaacgaact ggtgtggaac gtgctcaagg acaccgttgg
     3421 tgaggaagaa gctgtcttgt ttgcccgctc ggcctccgtc ggtgcgcaga aattcccggt
     3481 acactggggt ggcgattgtt acgctaacta cgaatcaatg gcggaaagcc tgcgcggtgg
     3541 tttgtctatt ggcctttcag gttttggctt ctggagccac tatatcggcg gctttgaaaa
     3601 taccgctccg gcgcacgttt acaaatgctg gtgcgcgttt ggtttgctct ccagccatag
     3661 ccgtttacac ggtagcaaat cttatcgtgt gccgtgggcc tacgatgatg agtcctgtga
     3721 tgtggtgcgc ttcttcacgc aactgaaatg ccgcatgatg ccgtatctgt atcgtgaagc
     3781 tgcgcgtgca aacgcgcggg gtacgccgat gatgcgggcc atgatgatgg agttcccgga
     3841 cgatccggct tgtgattacc ttgaccgtca atacatgtta ggcgacaacg tgatggttgc
     3901 gccggtgttc actgaagcgg gcgatgtgca gttctacctg ccggaaggtc gctggacaca
     3961 cctgtggcac aacgatgagc tcgacggtcg ttgctggcat aaacagcagc acagcttcct
     4021 gagtctgccc atttatgtgc gtgataacac cctactggcg ctgggcaaca acgatcaacg
     4081 tcccgattac gcgtggcacg aaggcacggc attccacctc tttaatctgc aagacgggca
     4141 tgaagccgtc tgtgaagtgc ccgctgctga cggatcggtg atctttactt taaaagcagc
     4201 acgtactggc aacacgatta ctgtgactgg tgcgggcgag gcgaagaact ggacactgtg
     4261 cctgcgcaat gttgtgaaag taaatggtct gcaagacggt tcgcaggctg aaagtgagca
     4321 ggggctggtg gtgaagcctc aagggaatgc gctgacaatt acgttgtaat ctattgatgg
     4381 aatgccgggt acgtctgagt cgtacccggc gagattgatg tttactgcac cagctctgcc
     4441 gacgtaactt caccgctgga aacgatgcca gcattcatgg tttgagtcac ttgctgcttt
     4501 gcggcgttca cggcatgcaa ttgcccgcta accgtaggtt tcagcggggc attggcctga
     4561 atatctccac tggcggtaag ctgaatattg ccgtcgccag tcaacggtaa ttcaggccat
     4621 ccccattgtt gcaaaatatt caccggcaca ccgcgtccat tcaggctgat atgggtctga
     4681 cgttgtggcg tttgtgaaac actggcagtg gcttccagaa tgcctttttc agtaaatgca
     4741 ctcagttcgc tgatattcac cgtgctgctg ttggcggtca gcgccagcga cgggcgacga
     4801 acatcaacac gattgaatgt cgcggcggcg gcattcagat tcgccgagcc actccagacg
     4861 ccccatttat gatcggtaac cagcgtcagg ttggcaccgt aaccatcgag cgtggtgagc
     4921 tgccacggga agtcagggtc gatatcaatg atcagattgc ggctggcgct aaatctcttc
     4981 agttgcaggc tgtttaacca accgggtgtc gtttccatcc acaactgttg ccagtttttc
     5041 ggcaaggtat attccagccc ggcaattgcc gcatcatcaa ggatcaacgt tttcccgtca
     5101 cgcagccagt tccctgacgt tctgaccata cccccttccc agcggctggt gaactggcgc
     5161 agcgctacgc cctgcgggga aaattccgcg tttataatcg ggtcaaataa atgcagcgaa
     5221 ccataaatga actcgctagc gttcatcaac agtttgcctt cttgtgtctg ccagtcatct
     5281 ttactgaagg tcatgttgcg caagctgaga tcgaggtcgg tcaccgccca gtccggacct
     5341 tgcaaatgag catcgatcac ttccaggcga ccaatctgca acgacgggac agagcgtaat
     5401 ggcgcaaaga agtcggttag cgatttttcg ctttgtagac gtatatccgc catgcgcagg
     5461 ttttccactt gccagctgcc gtcggcgtta cgctgcgcgt ttccggttaa tgtcccgcgg
     5521 gcgatgtcgg cacccaggtt agtcagcgta acgcgatcgt tatcaatact gccttcaatc
     5581 agtacattgg tggcaggaac atcattgagc gaaagcgatc cggcactaaa ctgaatctgc
     5641 gccttcgtac ccagcacttt accggctttt ggtgaccacg gaactacgcc gccatttacc
     5701 cgctgcgcgc tcagtttcca ttcgctattc gggctattaa acgccatatc acgcagttgc
     5761 agacgatcgg ctttgaacgg tagcggcgcg gtctggtcgg tgagattcag cgtcccgttt
     5821 tccagcagga tggtatcgac atggcgtggt tcggtcagtt gccgactgct tagcgcaatg
     5881 tcgacacttt tggccaccag ggtcgcgggc tgaccatcac gaccaaacgt gacattctcc
     5941 agcacgatat gagatggcgc ggaaaaacgg tgatccatcg ccccgaaggc cagatgatag
     6001 tcgctattct cggaaaccca tgcgctgata tgttctgctc cccagcgggt ttgcagaaga
     6061 aaatagaggc cagcgatcac cactaacaga gcgatgagaa tgtagagaag cagcttccca
     6121 ataaatttca tggtcttcca ttccgtgaaa tgcacataag agagttatgc acgatttacg
     6181 cgcaatcctc aaggcgggaa tggtgaaaga ggtgagactg cggcggtaat tatcttaccg
     6241 ccgccagtga attactgttt ttctggtggg aaaatcagat tcagcacgat ggcagtaata
     6301 ccgcccgcgg cgatcccgga ggagagcagg tttttcagcc attcaggggc aaactgcaaa
     6361 atcagcggct gctgagacac gcccagacca accgccagcg acagcgcgat aatcagaatc
     6421 gcccgacggt tcagcggctc acgagaaacg atacgcacac cggaggcggc gatggtgcca
     6481 aacattacaa gcgttgcgcc gcccagaacc ggttctggaa tgtgttgtac aaaaccgctc
     6541 actgccggga acagacccag cacgatcaac atcagcgcga cgacaaaacc gacatagcgg
     6601 ctggcaacac cagtcaactg gatcactccg ttgttttgcc cgaagcagga gttcgggaag
     6661 gtgttaaaca ccgccgaaac aaacgagttc aggccgtttg ccagcacgcc gcctttcagg
     6721 cgtttcatgt acagcggacc ggacactggc tgttcggaaa cgtcagaggt cgacgtgata
     6781 tcgccaatgg tttccagcga agtgatcata aagaccagca tcagcggcag cagcagactc
     6841 cattcaatgc caagaccgta atagagcggc gttggcacca taatcagttc ttgcgtcatc
     6901 ggttcgttgc tttctggcaa catgcccata aaccacgcca gcgcatatcc ggccgccatc
     6961 gcaatcacca gtgaggccac gcgtaagtaa gggttacgtt gacggttaag caggataatt
     7021 aaggctaaga ccacgcctgc cagcagcaga tttttcggtg cgccgaaggt gttatcgctc
     7081 atggctgcgt aaccgccgcc aatggacgtt aacccaacct gaattagcga caggccgata
     7141 atcatcacca caacgccaga aaccagcggc gtaataatgc ggcgcgccag atgcagaacg
     7201 cgggagatca ccatctcggt gcaacttgcc agcatcaacg tgccgaacaa agccgccatc
     7261 atggtaggaa catcagcacc accggttttc agcgcggtac cgcccataat cagcggggca
     7321 acaaagttga agctggtgcc ctgaatagac aacagcccgg agccaaccgg accccaggtc
     7381 ttaatttgaa taatcgatgc cacaccggag gcaaacagcg acatactaat aatgtgttgc
     7441 gtgtcttgtg ccggtaaacc cagcgcctgg cagattaata gcgctggcgt gatcaccgca
     7501 acgaacatcg ccagcagatg ctgacaggcg gcaaacaggg tttgaggaag cggcggacga
     7561 tcttcaagac ggtaaatcag ttcgctgttt tgagtctgcg caaccggttg cgcattttct
     7621 gactcgaggg tggaaacaga catcgaaaac aatcccgtgg tagcaaagcg ggcattttag
     7681 ctgaccgaaa gagaaaagca aacgtttgcc acatcctcat ctaaatattt aaatgagata
     7741 aatgcacttt tttatataac ttttggtatt tttctgctta aaatccatgc catttgttca
     7801 ttaacagctg agaatttgac ccaggaaagc ataactcgat acccgtcata gttcatgaag
     7861 ttgccactgc gcgatgaagt atgacgagta tgaaagagtg atgcggatac aaaggagtaa
     7921 ctatgtttca tctcgatact ttagcaacgc ttgttgccgc aacgctgacg ttgctgctcg
     7981 ggcgtaagtt ggtccattcc gtctcctttt tgaagaaata caccataccg gaacctgttg
     8041 cgggtggttt gttggtggcg ctggcgctac tagtactgaa aaaaagcatg ggctgggaag
     8101 tcaactttga tatgtccctg cgcgatccgt taatgctggc tttcttcgcc accattggcc
     8161 tgaacgccaa cattgccagt ttgcgtgccg gtgggcgtgt ggttggcatc ttcttgattg
     8221 tggttgttgg tctgttggtg atgcaaaatg ccattggcat tggtatggct agcctgttag
     8281 ggcttgatcc gctgatgggg ctgttggccg gttctattac gctttccggc ggtcacggta
     8341 cgggcgctgc gtggagtaaa ttgttcattg aacgttatgg cttcaccaat gcgacggaag
     8401 tggcgatggc ctgtgcaacg ttcggtctgg tgctgggcgg cttgattggc ggtccggtgg
     8461 cgcgctatct ggtgaaacac tccaccacgc cgaacggtat tccggatgac caggaagtcc
     8521 cgacggcgtt tgaaaagccg gatgtgggac gcatgatcac ctcgttggtg ctgattgaaa
     8581 ctatcgcgct gattgctatc tgcctgacgg tggggaaaat tgttgcgcaa cttttggctg
     8641 gcactgcttt tgaactgccg accttcgtct gtgtactgtt tgttggcgtg attctgagca
     8701 acggtctgtc aataatgggc ttttaccgcg tctttgagcg tgcggtatcc gtgctgggta
     8761 acgtaagctt gtcgttgttc ctggcgatgg cgttgatggg gctgaaactg tgggagctgg
     8821 cttcgctggc gctgccgatg ctggcgattc tggtggtaca gaccatcttc atggcgttgt
     8881 atgccatctt cgttacctgg cgcatgatgg gcaaaaacta cgatgcggca gtgctggctg
     8941 cgggtcactg tggttttggc ctcggtgcaa cgccaacggc aatcgccaac atgcaggcga
     9001 tcactgaacg ctttggcccg tcgcacatgg cgtttttggt ggtgccgatg gtcggtgcgt
     9061 tctttatcga tatcgtcaat gcgctggtaa ttaagttgta tttgatgttg ccgatttttg
     9121 ccggttaacc gatgaagcgg cggtcttctc accgccgcct tttacgcatt cgagtaacgt
     9181 tctgtctccg gcatccagcg ttctatcaag gcttttgcct gttgtgggta acgttcgtga
     9241 atatgtcgcg ccaggcgctg aacttccggg atcatcgcct gatcgcgcag taaatccgcc
     9301 actttaaatt cagcattacc cgtctgacgc gtgcctaaca attcgccagg gccgcgaatc
     9361 tccagatctt tttgcgcaat cacaaaaccg tcgttactgt cgcgcagcac ttgcaggcga
     9421 atttgcgccg ttttagaaag cggcgttttg tagagcagca cgcagtgaga agccaccgcg
     9481 ccacgaccta cgcgcccgcg cagctggtgt aactgcgcca gaccgagacg ctcagggttt
     9541 tcgataatca tcaggctggc gttaggcaca tcaacaccga cttcaataac ggtagtggca
     9601 accagcagat gtagctcacc ttgtttaaac gacgccatca ccgcctgttt ctcggctggt
     9661 ttcatccgcc cgtgtaccag gccaacattc aattctggta gcgccagttt caactcttcc
     9721 caggtagctt ccgccgcctg cgcttccagt aactctgact cttcaatcaa cgtacaaacc
     9781 cagtacgcct gacgaccttc agttatgcag gcatggcgca cgcggtcaat gatgtcggta
     9841 cggcgggtat caggaatagc gaccgtagtc actggcgtgc ggcctggcgg cagctcatct
     9901 atcaccgagg tatcgagatc ggcatacgcc gtcattgcca gcgtgcgggg gatcggcgtg
     9961 gcggtcatga tcaactgatg cggatggaag ccttgctgct ggcctttctc ccataacgcc
    10021 agacgctgat gcacgccaaa gcgatgttgt tcgtcgataa tcaccagcgc cagaccgtta
    10081 aactgcacct gttcctggaa gatggcgtgc gtaccaacaa tcatctgcac ctgaccgctg
    10141 gcgatggctt cctgctgaga cagtcgcgct ttgcctttct gcttaccggc gagccagccc
    10201 acttcgatac cgagcggttc aaaccagttg cggaagttat tggcgtgctg ttcggcgagt
    10261 aattcggttg gtgccatcaa tgccacctgt ttgccgtggg caatcgcacg cagcgcggcg
    10321 agggcggcga ccagcgtttt accggaacct acatcgccct gtaccaggcg catcatcggc
    10381 acatccagcg ccatatcgcg ctcgatctcc gccactacgc gtgcctgtgc gcccgttggc
    10441 ttgaacggta aggctgcgag gagtttattt ttcagcgcat cattggcgct cagcggctgg
    10501 gcatgaaaac gctgtgctcc ggcacgtaag gctaacatgc tgaggttgtg cgccagcagt
    10561 tcttccagaa tcagacgacg ttgcgccgga tgctgcccgg tttccagatc gctaagctgt
    10621 agcgtcggtg gcgggcggtg caaagtgcgc aacgcttccg gtagcgtcat cattccttgt
    10681 gacagttccg gcggcaggag ttcttcaatg gcgcaggtgt cgagcagatc cagcgcctgg
    10741 tcggttaatt tacgcagcgt ggcctgcttt acgccttccg ttgttggata aaccggcgtg
    10801 agcgtttcct gtaattctgg cgtgctgaga tcgccctgca cgcggtattc cgggtggatc
    10861 atctccgcac catatttacc gcgctttgct tcgccataag ccagtacacg gcggcccgtc
    10921 gccaggctat ttttcattgc cgcgctgaaa ttgaaaaagc gcatggtgag gatgccggaa
    10981 ccgtcgctga tctggcaggt catcatccgc cgaccgccga aggagatatt gcagttcagc
    11041 acttcgcctt ccaccgtggc ataaacgccc ggcagtagtt ctccgatggg gtagagatgg
    11101 gtgcgatctt cgtagcgcag gggaaggtgt aagagtagat cctgcacggt atgcaggttg
    11161 attttcgcca gtttgttact aagtgctgcg ccaacgcccg ttagggaact gagtgggaca
    11221 gcatctaaca ggcgacctgt catggcactt accctgcagc ctgcatagta gcccaccagt
    11281 cggcatcagc ttcgatctcg ccttgctgat tgacgtgggg ataaggcagg cctttgcgtt
    11341 ttgcgacttt cgccagcacc ggatagccgc cttcaaacaa caggcgttgt tgctctgctt
    11401 ccggcaacat gctgttttca cgcaggtaca tgcctgcatt ttgccgctga cgctgggctt
    11461 cgtaaagaat gagggctgag gcaacggaaa cattcagcga ctgcaccatg ccgatcatcg
    11521 gaatgatgat gtcctgatcc gccagggcca atgcttcctg cgtgatgccc gttttctcct
    11581 gtcccatcaa aatgcaggtc gggcgagtgt aatcaatttc gcggaaatcg acagcgttat
    11641 cagaaagatg ggttgccaga atctgcatgc cctggccttt gagatgagcg acggcatcgc
    11701 caatggtgcg gtgtgttttc acctgtaccc agctgttact acccgccgct gccgaagcca
    11761 tggtgcgcat gcggctacca ggccagacgg cgtgaacttc atgtacgcca acggcatctg
    11821 cggtacgaat aatcgcagaa acgttatgag gtttgtggac ctgctccatg cagacggtca
    11881 gatcaggctg ccgcctggcg agcatttcgc agatgcgtgc ataacgtgtt gggttcataa
    11941 aacattaatt tcggtttcgg gtgactttaa tcacgtctgg catcacgcgg attttgcgca
    12001 tgatattcgc cagatgcaca cggtcacgag cggtcagacg aataaaggcg ctgtagacgc
    12061 gaccatcttt ctcttccgta ttcaaacttt gaatattcga agtcgtggtg ttaattgccg
    12121 ccgtcaggtt tgccagcgca ccctgatgat tgaacatctc caccttgatt tcggtgatga
    12181 actcctgcgc cgtctcttta tcccattcca cagccataaa cttctctggc tctttctggt
    12241 agccacggat gttacggcag gattcatggt ggatcaccag acctttaccg gggctgacgt
    12301 gggcgataat cgggtcgcca ggaatagggc ggcagcattt cgcaaaggtg atcagcacgc
    12361 catccgcgcc tttgatcggc agatgcccgt ggctttgagt cgccggtgga atggaggcgt
    12421 ccccatgttg tagatttttc gcgaccacca cgctcattgc gttaccaagg ccgatttctg
    12481 ccagcagatc gtcaagcgtt gccagcttca tgcgatccag ctcgcgctga atattttcct
    12541 gcgggatttc attgagctta cggctaccac ccaaagcatg gttgagcaga cgacggccca
    12601 ggcttacaga atcatcacgc ttgaggtttt tcagcaactg acgaattttg gcgcgcgctt
    12661 tcgagctaac gacaaagttc agccaagcgg cattcgggcg agcgcccgga gcggtaatga
    12721 tttcaacggt ctgaccgcta gtaagcggct gcgacagcgg gtaaggctgg cggtcgacgc
    12781 gtgcgcccac gcaggcatgg ccgatatcgg tatgcactgc ataagcgaag tcgacgggcg
    12841 ttgcaccggc aggcagctcg acaatgcgcc cttccggtgt gaaaacgtaa atctcatccg
    12901 ggaagagatc ggatttaacg ctctcgataa attcaaacga actaccggcg ctctgttgca
    12961 gctccagcag gctttgcatc cagcgctggg cgcgtatttg tgcggtagta ctggtttcgc
    13021 cgtgctcttt ataagcccag tgcgcggcaa cacccatctc cgccatctga tccatatctt
    13081 cggtacggat ctggacctca accggcacgc cgtgcgggcc aatcatcgag gtgtgcaacg
    13141 actgatagcc gttcgctttt ggaatggcga tatagtcttt cacgcggccc ggacgcggct
    13201 tgtacaggct gtgcatctgg cccagcacgc gataacaggt gtcagaatca ttgacgatca
    13261 cgcggaaagc gtagatgtcc atgattgagt gaaaacgctg ctctttgagc accattttgc
    13321 agtaaatcga ataaagatgc ttttcgcgac cactgacgcg gcacggtatt cccgcttcct
    13381 gcaaacgccc ttcgatttca gaaagaatct tctggatcat ctctttacgg ttgccgcgcg
    13441 cggctttcac cacttctttg attacgcgat aacggttggg atacagcgcc tcaaaaccca
    13501 gctcttcgag ttcggtttta atgtggtgga tacctaaacg gtgcgccagc gggctgtaaa
    13561 tttcgagagt ttcacgggcg atgcggcgac gtttgtccgg gcgaagtgag cccagcgtgc
    13621 gcatgttgtg ggtacggtcg gcaagtttga tgaggatgac gcggatatcc tgcaccatcg
    13681 ccataatcat cttgcgaaag ttttcggcct gcgcctcttt cttatcgcgg aacttgagtt
    13741 tatcaagttt cgacaccccc tctaccagct cggcgacact tttaccaaaa agctgttcca
    13801 tatcctggta ggtggcggga gtatcttcaa tcacgtcatg cagcagcgcc gccatcagcg
    13861 tttcatagtc gagtttcatc tcggccagaa tgcaggcaac cgctaccggg tgcgtgatat
    13921 agggttcacc gcttgaacgt gtttgcccct cgtgagcatc acgtgcaacg agatacgcct
    13981 gccgcagacg cttgatttgg tcttccggca ggtaggtttg aatcagttga ttcaggcttt
    14041 caaacagata caagggcgac ccgctttgtg attaacgacg accttcagca atagcggtaa
    14101 cggcttgtaa ttcagcggct tcctgctctt gctgttcctg gcgttcgcga acgtcgagga
    14161 tctggttgtt gatcagacct tcttcgattt cgcgcagcgc gattacagtg gttttatcgt
    14221 tttcttccgg taccagcgga tcctttccgc ctacctgcat ctgacgagcg cgacgcgcgg
    14281 cgaccagtac caggtcaaaa cggttaccaa ttttctctac agcgtcctga acagttacgc
    14341 gtgccatact taaaaagctc cacaggtgaa gaaatgactg ggcatgatac tgaaatcagg
    14401 ttcagtctgc caacaatttg ctgattaaag cgtcatgacg ctgcttttgg cggctcatgc
    14461 gcagacgttc ggcgcgaata atggtcttca aatcggtcaa cgcggtatcg aagtcatcat
    14521 tcacaatcag gtaatcatat tcgtcgtaat ggctcatttc tgcaacagct tgcgccatac
    14581 gctttgcaat gacctcttcg ctgtcctgac cgcgaccgcg tagacggcgg tccagttcaa
    14641 ttttagacgg cggtaaaata aagatactcc gcgcgtgcgg catcttctgg cgaatttgct
    14701 gcgcgccctg ccagtcgata tcgagaaaaa catcgacacc ggtcgccagt acttgctcaa
    14761 tggcctcacg cgaagtgcca tagtaattac caaaaacttc tgcgtgttcg aggaacgcat
    14821 ctctgctaat catttcttta aattcatcat gattaacaaa gaaataatgt tcaccgtgga
    14881 cttcaccagg acgcggttgg cgtgtggtgt gtgaaacaga aacctgggtg tcatacaacg
    14941 gttgggtttt taataaagcc tgaatcaggc tggatttacc cgcgccactg ggggcagaaa
    15001 caatataaag cgtgccttga gccatgagta tctttcgtat gtgattagcg aaataaagcc
    15061 tacatacgag cttattatac acggcgcggc catgtgacgt agcccttgct cacacttttt
    15121 gcgcaaagtt gttgcgtttt gcgtagctgc ttccgcattt gcctgtgatt tactgcaata
    15181 aagacgattc tcaggaagcg tcctcgcaga ctccgttttt gccactcggc tttgccttca
    15241 ccacgttgta tatctgccag cctgactaca gcggaggtga agatgaaagt atggatggcg
    15301 atattaataa gtatcttgtg ctggcaatca tctgtgtggg cggtctgtcc ggcctggtcg
    15361 ccagccagag cacaggaaga aattttccgc cagcaacagc aaataaaaca gtgggacgat
    15421 gactactgga aggaaggaaa aagtgaggtg gaagacggtg tttacgatca gttaagcgcc
    15481 cgtcttacgc agtggcaacg ctgctttgtg agcgagcccc gcgacgtgat gatgccgcct
    15541 ttaaatggtg cggttatgca tccggttgcc catacgggtg tacgcaaaat ggcggataaa
    15601 aacgcattaa gtctgtggat gcgagaacgt agcgatcttt gggtgcagcc aaaagttgat
    15661 ggcgttgcgg taaccctggt ttatcgggac gggaaactga acaaagcaat cagtcgcggt
    15721 aatggcctga aaggcgagga ctggacgcag aaagttagct taatttccgc tgtgccgcaa
    15781 accgttagcg ggcctttagc caacagtacg cttcaggggg aaatatttct ccagcgcgag
    15841 gggcatatcc aacaacaaat ggggggaata aatgcccgcg caaaagttgc tggcttgatg
    15901 atgcgccagg acgatagcga cacgctgaat tctctgggcg tttttgtctg ggcatggccg
    15961 gatggaccgc agttaatgtc tgatcgttta aaagagctgg ctaccgcagg ttttactcta
    16021 acgcagacgt atacccgtgc ggtgaaaaat gctgatgaag ttgcgcgcgt acgcaatgag
    16081 tggtggaaag cggaattacc cttcgtcacc gatggcgtag ttgtacgagc ggcgaaagag
    16141 ccagaatccc gccattggct accgggccag gcagagtggc tggtggcctg gaaatatcaa
    16201 cctgtagctc aggttgccga agtgaaggcg attcagtttg cggtgggtaa gagcggtaaa
    16261 atatcggtgg ttgcgtcact cgcatctgtc atgctggatg ataaaaaagt ccagcgggtg
    16321 aatattggtt ccgtcaggcg ctggcaggag tgggatattg cgcctggtga tcagattctc
    16381 gtcagccttg ccggtcaggg tattcctcgc attgatgatg tggtgtggcg cggtgcagaa
    16441 cgtacaaaac cgacaccgcc agaaaaccgc tttaactcgt tgacctgcta ctttgcttct
    16501 gatgtttgtc aggaacagtt catttcacgc ttagtctg
//
LOCUS       DADLFA010000088        16519 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000088 DADLFA010000000
VERSION     DADLFA010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16519
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.088"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            93..2321
                     /gene="crfC"
                     /locus_tag="JQH65_002379"
     CDS             93..2321
                     /gene="crfC"
                     /locus_tag="JQH65_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HAY6139767.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAAQLAL
                     PWSQPNIAERYAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHVNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQLD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            2318..3201
                     /locus_tag="JQH65_002380"
                     /pseudo
     CDS             2318..3201
                     /locus_tag="JQH65_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3465..4967
                     /gene="proP"
                     /locus_tag="JQH65_002381"
     CDS             3465..4967
                     /gene="proP"
                     /locus_tag="JQH65_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HAY6139768.1"
                     /translation="MLKRKKVKPITLRDVTIINDSKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            5033..5170
                     /gene="pmrR"
                     /locus_tag="JQH65_002382"
     CDS             5033..5170
                     /gene="pmrR"
                     /locus_tag="JQH65_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HAY6139769.1"
                     /translation="MSDAHTLLQVNGGECNEKPCLYESLTTVFSVLVVSSFLYIWFAT
                     F"
     gene            complement(5146..6246)
                     /gene="pmrB"
                     /locus_tag="JQH65_002383"
     CDS             complement(5146..6246)
                     /gene="pmrB"
                     /locus_tag="JQH65_002383"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HAY6139770.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(6247..6915)
                     /gene="basR"
                     /locus_tag="JQH65_002384"
     CDS             complement(6247..6915)
                     /gene="basR"
                     /locus_tag="JQH65_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HAY6139771.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKSRIRTVRGFGYMLVA
                     NEEN"
     gene            complement(6912..8555)
                     /gene="eptA"
                     /locus_tag="JQH65_002385"
     CDS             complement(6912..8555)
                     /gene="eptA"
                     /locus_tag="JQH65_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HAY6139772.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTV
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            complement(8659..9996)
                     /gene="adiC"
                     /locus_tag="JQH65_002386"
     CDS             complement(8659..9996)
                     /gene="adiC"
                     /locus_tag="JQH65_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HAY6139773.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTPGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            complement(10133..10894)
                     /locus_tag="JQH65_002387"
     CDS             complement(10133..10894)
                     /locus_tag="JQH65_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY6139774.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(11219..13486)
                     /gene="adiA"
                     /locus_tag="JQH65_002388"
     CDS             complement(11219..13486)
                     /gene="adiA"
                     /locus_tag="JQH65_002388"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HAY6139775.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTTDFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     YWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHAMCVKA"
     gene            complement(13685..14593)
                     /gene="melR"
                     /locus_tag="JQH65_002389"
     CDS             complement(13685..14593)
                     /gene="melR"
                     /locus_tag="JQH65_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HAY6139776.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMMIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            14876..16231
                     /gene="melA"
                     /locus_tag="JQH65_002390"
     CDS             14876..16231
                     /gene="melA"
                     /locus_tag="JQH65_002390"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAY6139777.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATLLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPSTLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPHIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGESSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            16334..>16519
                     /locus_tag="JQH65_002391"
     CDS             16334..>16519
                     /locus_tag="JQH65_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HAY6139778.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIM"
BASE COUNT         3986 a         4001 c         4348 g         4184 t
ORIGIN      
        1 tttcttgcag taattataaa agccgaaaac agaactcaaa aacaatctgg ctaaataaaa
       61 gtaacaaaat ttgctttaag gaagaatttt ctatgtacac acagaccctg tatgagttaa
      121 gtcaggaggc tgaacgcctg ttacagcttt ctcgccaaca gttgcagtta ctggaaaaaa
      181 tgcctctctc tgtacccgga gacgatgcgg cacaactggc tttaccctgg agtcagccta
      241 atatcgccga acgttacgcg atgctgaata atgagttgcg taaaatttcc cgactggaaa
      301 tggtgctggc gattgtcggt actatgaaag cagggaaatc aaccaccatt aatgccattg
      361 ttggtacgga ggttctgcct aatcgtaatc gcccaatgac tgcgctgccg acgcttattc
      421 gccatacgcc cgggcaaaag gaaccggtac tgcatttttc acatgtcgcg ccaatcgatt
      481 gtttaattca acaattacaa cagcgcctgc gtgattgcga tattaagcat ctgaccgatg
      541 tgctggaaat agataaagat atgcgtgcgc ttatgcagcg gatcgaaaat ggcgtcgctt
      601 tcgaaaaata ttatctgggt gcccagccta tttttcattg tctgaaaagt ttgaatgatt
      661 tagtgcgact ggcgaaggcg ctggacgtcg attttccttt ttctgcttac gccgccattg
      721 agcatattcc cgtgattgaa gtggagtttg tccatctggc ggggctggaa agttatcccg
      781 gtcagttgac gttactggat acccccgggc caaatgaagc cgggcaaccg catctgcaaa
      841 aaatgcttaa ccagcagctg gcacgcgcct cggcggtact ggcggtgctg gattatacgc
      901 aactgaaatc gatctccgat gaagaggtcc gtgaggcgat tttggcggtg gggcaatcgg
      961 tgccgctgta tgtgctggtc aataagttcg atcaacagga tcgtaacagt gacgacgccg
     1021 accaggtgcg ggcactgatt tccgggacgc tgatgaaagg ctgtattacg ccacagcaga
     1081 tatttccggt gtcgtcgatg tggggctacc tggcgaatcg ggcgcgctat gagttagcca
     1141 acaacggtaa gttaccagcg ccagagcaac aacgctgggt ggaagatttt gcccatgccg
     1201 cgctcggcag gcgctggcgt catgccgatc tggcggacct cgaacatatt cgtcatgctg
     1261 ccgatcagtt gtgggaagat tcgctgttcg cccagccaat tcaggcgttg cttcatgccg
     1321 cttacgctaa cgcctcattg tatgctctgc gatctgctgc gcataaactg ttgaattacg
     1381 cgcagcaggc gcgggaatac ctggattttc gtgcgcacgg gttaaacgtc gcttgtgaac
     1441 aattgcggca aaatatccat caggtcgaag aaagtttgca gctattgcaa ctcaatcagg
     1501 cgcaggtgag cggcgagatt aaacatgaaa tcgagctggc cctgacctcc gccaaccact
     1561 ttctgcgtca acagcaagat gcgctgaatg cgcagttagc cgccttgttt caggatgatt
     1621 cggagccgtt aagcgagatt cgtacctgct gtgagacact gttacagacg gcgcagaaca
     1681 ccatcagtcg cgactttacg ctgcgttttg ccgagcttga atccaccctt tgccgggtgt
     1741 taaccgatgt tattcggccc attgagcaac aagtcaaaat ggaattgagc gagtcagggt
     1801 ttcgtcctgg gtttcatttt cctgtttttc acggagtagt tccccacgtc aacactcgcc
     1861 agctgttcag tgaagtcatt tcgcgccagg aggcaacgga cgagcagagc acgcgtttag
     1921 gcgttgtgcg tgagactttt tcgcgctggt tgaatcagct cgactgggga cggggaaatg
     1981 agaaatcccc gacagaaacg gttgattaca gtgtgttgca acgagcatta agcgcagaag
     2041 tcgatcttta ttgccaacaa atggctaaag ttctggcaga gcaggtcgat gaatctgtta
     2101 cggcaggcat gaatactttt ttcgctgagt tcgcttcatg tttgacggaa ttacagacgc
     2161 gtttacgcga aagcctggct ctgcgtcaac aaaatgaatc ggtggtcagg ctgatgcagc
     2221 agcaattgca gcagactgtg atgactcacg gctggattta caccgacgcc cagctgttac
     2281 gcgatgatat tcaaacactt ttcacggcag aacgatattg accaagacgt tactggacgg
     2341 ccccggtcgc gtgctggagt cggtttatcc ccgcttttta gtggatctgg cgcagggcga
     2401 tgatgcccgc cttccacaag cccatcagca gcagtttcgt gaacgactga tgcaggaact
     2461 tctttcgcgt gtgcagcttc agacatggac gaacggcggc atgttaaatg cgccgcttag
     2521 cctgcgactg acaatggtgg aaaaactggc gtcgatgctg gatcccggtc atctggcgct
     2581 gacgcagatc gcgcagcatc tggcgctgct gcaaaaaatg gatcaccgcc agcactctgc
     2641 tttcccggag ctcccccagc aaattgccgc cttgtatgag tggttttcag cccgttgtcg
     2701 ctggaaggaa aaggcgttaa cgcaacgagg ccaggcctac tggtgcaggc aggtgatcag
     2761 agcgagcaaa tttttacccg ctggcgtgct ggggcgtata acgcctggtc gttgcctggg
     2821 cgctgtttta tcgttctcga agagttgcgt tggggggcat ttggcgatgc ctgtcgtctg
     2881 ggaagcccgc aagcggtggc gttgctgctg ggtgatttgc gcgtgaaagc gacacaacat
     2941 ctggcagaga gtatcaatgc ggccccgacc acgcgtcact attaccatca gtggtttgcc
     3001 tcttcgaccg ttccgacggg cggggatcat gctgattttt taagttggct gggaaagtgg
     3061 accacggcag ataaacaacc cgtttgctgg tcagtgaccc aacgttggca aactgtcgcg
     3121 ctggggatgc cacgactctg ttcagcgcag cgtttggtgg gggcaatggt cgaggaaatc
     3181 ttctctgtaa atttggcgta aataatcagt tacatcaatg agtcctaaac gaaatccatg
     3241 tgtgaagttg atcacaaatt taaatactgg tagggtaaaa aggtcattaa ctgcccaatt
     3301 caggcgtcaa ctggtttgat tgtacattcc ttaaccggag ggtgtaagca aacccgctac
     3361 gcttgttaca gagattgcat cctgcaattc ccgctcccct tttgcggccg tcgcgctgat
     3421 ttttctggcg tttgcggaaa tgggccaact ctgcgaggaa agctatgctg aaaaggaaaa
     3481 aagtaaaacc gattaccctt cgtgatgtca ccattattaa tgacagtaaa ctgcgtaaag
     3541 ccattaccgc agcatcactg ggtaatgcaa tggaatggtt cgattttggt gtttatggtt
     3601 ttgttgctta cgcattaggt aaagtttttt tcccgggggc tgaccccagc gtgcagatgg
     3661 ttgctgcact tgccactttc tccgttccct ttctgattcg accgcttggc ggactcttct
     3721 ttggtatgtt gggcgataaa tatggtcgcc agaagatcct cgctatcact attgtgatta
     3781 tgtcgatcag tacgttctgt attggcttaa taccgtccta cgacacgatt ggtatttggg
     3841 caccgattct gctgttgatc tgtaagatgg cacaaggttt ctcggtcggc ggtgaatata
     3901 ccggggcgtc gatatttgtt gcggaatact cccctgaccg taaacgtggc tttatgggca
     3961 gctggctgga cttcggttct attgccgggt ttgtgctggg tgcgggcgtg gtggtgttaa
     4021 tttcgaccat tgtcggcgaa gcgaacttcc tcgactgggg ctggcgtatt ccgttcttta
     4081 ttgctctgcc gttagggatt atcgggcttt acctgcgcca tgcgctggaa gaaactccgg
     4141 cgttccagca gcatgttgat aaactggaac agggcgaccg cgaaggtttg caggatggcc
     4201 cgaaagtctc gtttaaagag attgccacta aatactggcg cagcctgttg acatgtattg
     4261 gtctggtaat tgccaccaac gtgacttact acatgttgct gacctatatg ccgagttatt
     4321 tgtcgcataa cctgcattac tccgaagacc acggggtgct gattattatc gccattatga
     4381 tcggtatgct gtttgtccag ccggtgatgg gcttgctgag tgaccgtttt ggccgtcgtc
     4441 cgtttgtgct acttggtagt gttgccctgt ttgtgttggc gatcccggcg tttattctga
     4501 ttaacagtaa cgtcatcggc ctgatttttg ccgggttact gatgctggcg gtgatcctta
     4561 actgctttac gggcgttatg gcttctacct tgccagcgat gttcccgacg catatccgtt
     4621 acagcgcgct ggcggcggca tttaatattt cggtgctggt tgccggtctg acgccaacac
     4681 tggcggcctg gctggtcgaa agctcgcaga atctgatgat gcctgcctat tacctgatgg
     4741 tagtggcggt gattggttta atcaccggcg taaccatgaa agagacggca aatcgtccgt
     4801 tgaaaggtgc gacaccggcg gcgtcagata tacaggaagc gaaggaaatt ctcgtcgagc
     4861 attacgataa tatcgagcag aaaatcgata atattgacca cgagattgcc gatttgcagg
     4921 cgaaacgtac ccgcctggtg cagcaacatc cgcgaattga tgaataagct gaaacggatg
     4981 gcctgatgtg acgctgtctt atcaggccaa ttgaactctt aaggttcact taatctctga
     5041 cgcgcatact ctcctccagg ttaacggagg agagtgcaat gaaaaaccgt gtttatatga
     5101 aagtttaact accgtgttca gcgtgctggt ggtcagcagc tttctttata tctggtttgc
     5161 cacgttctga tctttcttca gccgtaccca ggcccgcgtg ccggaagtct cttgccggtt
     5221 ttgcaggaaa aactgcccgt gatgcaactg tgtaatgcgg ctgacaatac ttaaccccag
     5281 accaatcccg ccataacggc tgtccatacg tacaaacgct ttactcaact ccccgcattt
     5341 actctcatca atacctggtc cttcatcttc aactgccatg accgctccgc catcttcttg
     5401 cagcttaatc ataatgttgc tgccttgcgg gctgtaacga tgggcgtttt ctaccaggtt
     5461 tcgcaataac atccgcagca gggttgcatc accctgaacg gtgatgtcgg cggcgctctc
     5521 tggcaatagc agggtttgct gtcgctggtc gagcatggta ctgagttcgt catacgaggg
     5581 gagaatgaca tcttccagca gttttacatg ttgataatta ccggaagaaa atgactgtcc
     5641 ggcacgcgcc agttgcagca gctgggagac gctctccatc atctgatcaa gccgtgccac
     5701 taacggtgct acatcaatgt gatgcgtttt cgccagcagt tccagatgca aacgcacccc
     5761 cgccagtggc gttcgcagtt cgtgcgcgac gtcagcggta aacaaccttt cgttatccag
     5821 cgtgctggtc aggcgactga ccagatcgtt caacgccgaa accaccgctt cgatttcgag
     5881 ggtggcgctg tgaatggcaa tgggcgttaa gttgtcggca gtgcgcgctt ccagctcttt
     5941 ttgcagctcc gccagcgggc gggtgatgcg gcgtaccgcc tgatagcaga taaatagcgt
     6001 caggctgacc ataaagacgc cggggacaat caggctggcg accgcctcgc ggatctcacg
     6061 catgatgtgg cgatcgttgt tgcgattgtc gcgtagcgcc tgctcaaaca gctgaatctg
     6121 ctcggtactt tcatgccata gccagaagac gctgatcagc tcaaacacca acaaaatggc
     6181 cccgatagtc aatatcagcc gttggcgcag cgatattggt cggcgcagaa aacgcatcag
     6241 attcaattag ttttcctcat tcgcgaccag catatagcca aagccgcgca cggtgcggat
     6301 acgggatttg cccactttgt cgcgcagatt gtggatatgc acttccaggg tgttggtcga
     6361 gggttcattg tcccagttat agatgtcgtt gtagagaatt tcccgatgta ccggactgcc
     6421 tgctttgagc attaaccgtg acagcagagc atattctttg ggcgtcagaa tcaactcttc
     6481 accgcccatc catacctgac ggcgacccat attcagcgtc agattgccaa caatcagctc
     6541 actttcgccc tgattattat ggcgtcgtag cagggcgcgg atacgggcgt gtaactcttc
     6601 cagcgcaaaa ggcttcacca gatagtcatc ggcaccgaca tccagcccgg cgattttgtc
     6661 ggtcagcgta tcgcgagcgg tgaggatcag taccggcagg gtatattttt tctgccggat
     6721 acgggcgaga aaatgcagtc catcttcgtc gggtaaccct aaatccagta ccaccaggct
     6781 gtaatgaccg gcttcaaggc tttgttccgc catccgcgcg gttgtcacgc catcgcacgc
     6841 gtagccttcg gtttgcgccg ccagaatcag tccctgcaat aacagcgtat cgtcttcaac
     6901 aatcagaatt ttcatttact cactctcctg caagtttgca gaatatcatc cgcagcctgg
     6961 taatacttcg tctcaacgcc agttaatccc aatagcgtgg agaataaatt gtcttgtgaa
     7021 tagtgttgcg tttgcgcctg tttttgcagg cagttctggt caacctgata ccgtttttga
     7081 taatcctccg acagccacag cagcatcggc acctgttttt ggctatccgg ggcgatggca
     7141 taaggcagac cgtgcagata gatgccattt tcacctaacg attcaccgtg gtcagaaaga
     7201 taaaccaggc tggtggtaaa tttatcctga tgttctttca gcagattaat cgctttatca
     7261 acaatatagt cgacgtaaac cagcgtgttg tcgtaagtgt tcaccagttg ctctttggta
     7321 caggtctgga tctcattggt gtcgcaggtt ggggtaaatt tcctgaactg cggcggatag
     7381 cggttgtaat aggtcggacc gtggctgccg atggtgtgta agacaatcac gccatcaccc
     7441 tgcaggttat tgatgtactc ttccagcccg tggaacagca cttcgtcata gcattcgccg
     7501 ttgatgcact gaccaggcag attcagcgcg gtgacgttct ggtgaggcac gcggtcgcag
     7561 gcacctttac agccgccatc gttgtcattc cacagcacgt tgatgcccgc tcgctgaatg
     7621 atatccagca cgccttcctg gtgctgtgcc agctcttctt tgtaatgctc acgcggcata
     7681 tccgagaaca tgcacggtac tgaaactgcc gttgccgtgc cgcaagatac ggtattaggg
     7741 aaatagacca cgttatcttt cgccagccgc gggttagttt cacgcgggta gccgttgagg
     7801 gagaagttct ccgcccgcga ggtttcgccg acaatcagga tggtcaaatt tttacgtttt
     7861 tcgttctgca ttaacgggtt gcggtgggcg tcttcaccaa ttcgcaccag cggtagattt
     7921 gccagtcgct gatgggagta ccatgaccag ctggcaacaa tgctgttaga ggggcttaag
     7981 gatttcacca gctctttgtt attgcggaac aacgaggcgt agtctttata aaacagtgcg
     8041 gcgaccagca aaatcagtag tacagaaacc agaatattgg ctccacggaa aagaacactg
     8101 cgcagacgcg aggtggcagg tttgattttt atccagcagg caatcagcgc agcaagcacg
     8161 ccgctgaatc ccagcgttaa taacatttgc ggtgtcatca gcgcataact ttctgccgga
     8221 gtggtatcaa taatattggc aatcatcgag cggtcgatga cgatgccgta agtcattatg
     8281 aaatattgtg cagccgcgcc aaccagaata aacaggcagg ccagtggtcg attaagccat
     8341 aagaaagagc ttagtgtcag gacaatatta atcacgctga aagcgacgac cggcatcgac
     8401 aagaaaacca gtacgttatg cagcgaatcc agcggcagcg cctgcaacac ctgtttaaaa
     8461 aaggcaatat tcaggcagat agagatataa aaagcggcca acaatagcca ggcgagtaaa
     8521 ttcaaagagg gtctttttag taggcgcttc aacatcacgg tgtttccatc gaacaaagtg
     8581 cgcatatgct cgcaaagcaa aattaagcca accttaagtt cttaaggttg gcttttatgt
     8641 ttgctggatt aaggggaatt aatctttgct tattggtgca tctaagggat acgggttttt
     8701 atgtagccgg ttgtaattca gcgcatacat ggcggtgatg accatcaggg tgacaaatga
     8761 ccacataacc tctttcgctc cggaccccac cacggcccag atgcagtaga ggaaggcaat
     8821 ggtagtaact gccagatatg ccgggcgtgc tttaccaaag tgaccgtgtc cgagcagcag
     8881 taacgccgca caggtgtaaa gatatggcac cagtgtaaag atgaccgaga cggaagaaac
     8941 cagaccgaac tctttggtcg cgtttggtga aatgctgctg agctggaaga tggtcatcaa
     9001 aataccgacg ataatcagcc ccgccactgg cgtacccgct ttatttacac gggcaaaaat
     9061 cggtgggaac agtccgtcat cggcagcggc tttcgccgtt tgacccgcca gcaacgtcca
     9121 gccgcccagt gaacctaagc aacccgcagc tgcgcagaag gaaacaatgg ccccgggggt
     9181 gtcacccaac gccatccgtg cggcatcacc gaatggcgaa gcagaaacgc gcagtgcggc
     9241 attagggatc atccccataa tcgcggtggt agaaagtaca tagcaaacgg cggcaatcaa
     9301 taccccacca atggtggcga tagggacatt gcgtttcggg tttttcacca cacctgcggc
     9361 aacggaggca ctttccacac cgatgaacga ccacagcgta acgttaaggg tactttgaat
     9421 tgcaccgaag gtacccaggc cgctgacgtt ccatgccgcc atataggttt caccacggaa
     9481 ccagaaccag ccaaatacgg caatcccgac gatggggatc agcgccagca cggtggcaac
     9541 tgcctgcaca cgggtgatca ttttcggacc aacaatgttc agcaggacga agatccacag
     9601 caccacgacg caggtgatgg ttaataccaa tggatctttc agaatcggga agaagtaact
     9661 taaatatcct acgccaatga ccaccatggc gatattgccg atccagcagg ccagccagta
     9721 gaggacgttg gtttgataac cgagaaacgg gccaaagcag cggcgggcgt aagcgtaaga
     9781 accaccagga cttgggtcga ggaacgacat tttggcgtat accatcgaga gccccagtgc
     9841 accgataatc gtcaccaacc atccataaat ggcaatcccg ccagtggagg ccaggtttgc
     9901 aggtaacaga aaaacacctg accccataat attccccgac accatcaggg tgacggggat
     9961 taagcccact ttgtgagcat cagcatccga agacataatt aaactcctgc gaaggcgagc
    10021 ttcgtgacaa taaattacgt catatcatac gcctgcattc gatagttgaa ttattatcga
    10081 ccgggttatc gctgatgccg ttattaatat agctcttcgt taaggttaaa aatcaggcga
    10141 cagtgcgttc tctgtgctga ctaacataat gcagcggcgt cataccgtag aagtctttaa
    10201 agacagaaat aaagtacgac gtactgttgt agccgcagga ctgtgatacc tgagagatat
    10261 ttttaccgtc catcattaat tcatttacgg cataacgcat gcggcaggtg gtgattattt
    10321 ggctataact ggtgttttcg cttttcagct ttttctttaa cagacttggg ctaagacata
    10381 aacaactggc taccatacta agattccagt ctttgtgaat atcgctttca ataatttgat
    10441 aaacgctgtc acttacacaa ttacgtaaca tatacatcat taatgaaagg aattttttac
    10501 tgtcgagaaa acgggatagc accgtgaata ataatgcgcg tgttcgttcc ttttctgact
    10561 cagtttctgc gggcatcatg ctgtgttgcg cggcaacgcg aaagacgtct ggcgtcaggc
    10621 atggcagggt gagtatgggc gtagcgctac gttgccatac tggtatttgc gagagatctt
    10681 tattcagaaa ccggaggtaa tctttgatga tgtcgtgact aatatgagca accaaagtgt
    10741 tgttaagaga ggagacgtcg ataatattat tttcacaatt taataacgcc atatggttag
    10801 ctttaaggct aataggttct ttcccattca cccttatcca gacatctttt tcagtcaata
    10861 aaacaataca aggttggtcg ctgcaaatcc tcatacatgt actcctgagt gcgaataaaa
    10921 gaccagttct cgctaaagca aagcgatacc gggaaatggt tatataattg tatgacgaat
    10981 ataaacatgc ataaaaataa cgagttttac taatattaca aatttttata gtaatttctc
    11041 atgtacatat ttttttacag ggtaaataac aggcaaaaaa aagcgcgggg tggggccgcg
    11101 caaaaagtat gacatttttg tttgcagcaa tttttatgaa aacgttgcca tactcaaaag
    11161 gcctaaggcc aggcggcgta atgttattta aacaattacg ccttcagcgg aatagtggtt
    11221 acgctttcac acacatagcg tggtaaatac cgtcaataat ttcagtccct tcagtttcgt
    11281 gttcaaatcc agggaaatgg tggtcccagg attgcagcga gcgtaaataa cttacttgcg
    11341 gactgttttt atcgccgaag ttttcaccag acagcagcat cgggattcct ggcggatatg
    11401 ggataactga gtttgccgcg atgcgtcctg gcagattttc aatggatacc agttcgacat
    11461 tgttgtcgac aatcgcgttg tacgcttcac gcggggtgat ttccgccacc ggcaggccgg
    11521 aataggcttc gttcaaccgt gcgccagggt tgttttcttt cagccaggca aacatggtgt
    11581 cacccagatc gtgaatcccc atgttcgcgt aagtgtcagg atattgttca acaagttccg
    11641 gcatcacctg cgccagcggt gtgttggcgt catagtggcg tttgaaggag caaagggtgt
    11701 taaccagagt cccccatttc ccacgggtta cgcccataga gaacaggaac ataatttgga
    11761 agtcagtggt gcgggtaggc acaatgccgt ggcgaccaag ccaggcagtg accagcgccg
    11821 ccggaacacc ggtttcttcc agttcaccat cttcacccat tcccggagca aggatgctga
    11881 ctttaatcgg gtcgagcata ctccagttat ccggaatatc tttgaagccg tgccagcttt
    11941 cgcccggatg cattacccag tagtcctgaa cggtggtcag cagtttggtt ggtgcgtcag
    12001 caaagtcata ggttttgccg gtttgtgggt cggtgacgac ttctttgttc cacggtttga
    12061 agaaccagct accgtcagcg gtgaactctt tatacagccg cgccatcgcc tgacggaaat
    12121 caaccgcttc gtcgatcact tcctgcgtca gtgacaggcc gctgttgccg tccatcatcg
    12181 acaccgccac gtcgttggat gcgcagatgg catacagcgg ggaggtggtg gcatgcatca
    12241 tgtaggcctg gttgaagcgg gagaagttaa tcgccccacg accttcacgt acatgaatat
    12301 aagaagcctg tgaaagcgca ttcagcagtt tgtgggtgga gtgggtggcg aaaacggtag
    12361 gaccgttgtg atcgccaggt tcgccgcgca tggcatagtg atcggcatag atcgggttga
    12421 aacgtgcata gccgtaccag gcttcgtcaa agtgcagacg atcggaggtt ttttccagca
    12481 gatcctgcgc ttctttagcg ttataacaca cgccgtcata ggtgcagttg gtcaccacgc
    12541 agtaagacgg tttttgcccg gctttgtctt tagtcagcgg gctttcactg attttcttct
    12601 gcaaggtttc aggttgcatt tcctgcggat agattggccc gataatgccg tagcggttgc
    12661 ggcttggcac catgtagacc ggtttcgcgc ctgtcagcat caagccttgt tcgatggatt
    12721 tatggcagtt acggtcaacg accacgacat cgttatcggt catgcaagcc tgcatgatgg
    12781 tgcggttaga gccggaagta ccgacgacta ccgaccagga gcgatcggca ccaaatacgc
    12841 gtgcggcata tttttcgctt tcgccaaatg cgccagtatg gtcaagcaaa gaaccgaggg
    12901 aagttcgttc gatgcccata tcggtgcgga acagattttc tccatagtag tcatggtaga
    12961 aacgtccggc gggtgttttg gtaaaaccaa cgccgccctg gtggcctggc gctgcccagg
    13021 aatattcatg gatgtcgcta tacttcatca gcgcgctgaa cagtggcggc aacagctgct
    13081 ggcggtagcg ggtcatcgcg gcaacggcgc gtccggcgat aaagtcggtg gtatcttcca
    13141 gaatccaggc gaattcatcg acaagctcca gcaggtcgcg atccattgcg gcgagggctt
    13201 tttcccgatc gcccaacagg aagaccggca cgttttgttg gcgctcatga agcttaccga
    13261 tcaattgtct gacgttttga tgttcgtccg gatgttccat ttgatagctg aacatcaggc
    13321 agtcaatggc ttcgtttgaa gagagaatgg caaaaccatc atcaaaggag gtggatttaa
    13381 tcacggtaac attttgctgg cttaaagcat ctgccagacg ctcaacggcg ttaccgaccc
    13441 aggtgtcttg atggagaaac tcgctttcaa caattaatac tttcatcatt gcttacccgg
    13501 ttatgaagga atcttcgttg cgggcaagta tcttccggcc ttttcgggct gtaaagcgcg
    13561 tgaaaaacaa acatttttga aatatttatt tttataaaca atgcgttatg tgattatttt
    13621 aaattatttt cactgcttgt tatcgacggc taaactattt ttttggctga tactgatatc
    13681 gtctttagcc gggaaacgtc tggcggcgct gttggctaag tttgcggtat tgttgcggcg
    13741 acatgccgac atatttgccg aacgtgctgt aaaaacgact acttgaacga aagcctgccg
    13801 tcagggcaat atcgagaata cttttatcgg tatcgctcag taacgcgcga acgtggttga
    13861 tgcgcatcgc ggtaatgtac tgtttcatcg tcaattgcat gacccgctgg aatatcccca
    13921 ttgcatagtt ggcgttaagt ttgacgtgct cagccacatc gttgatggtc agcgcctgat
    13981 catagttttc ggcaataaag cccagcatct ggctaacata aaattgcgca tggcgcgaga
    14041 cgctgttttt gtgtgtgcgc gaggttttat tgaccagaat cggttcccag ccagagaggc
    14101 taaatcgctt gagcatcagg ccaatttcat caatggcgag ctggcgaatt tgctcgttcg
    14161 gactgtttaa ttcctgctgc cagcggcgca cttcaaacgg gctaagttgc tgtgtggcca
    14221 gtgatttgat catcatgccg tgagtgacgt ggttaatcag gtctttatcc agcggccagg
    14281 agagaaacag atgcatcggc agattaaaaa tcgccatgct ctgacaggtt ccggtatctg
    14341 ttagttggtg cggtgtacag gcccagaaca gcgtgatatg accctgattg atattcactt
    14401 tttcattgtt aatcaggtat tccacatcgc catcgaaagg cacattcact tcgacctgac
    14461 catgccagtg gctggtgggc atgatatgcg gtgcgcgaaa ctcaatctcc atccgctggt
    14521 attccgaata cagcgacagc gggctgcggg tctgtttttc gtcgctgctg cacataaacg
    14581 tatctgtatt catggatggc tctctttcct ggaatatcag aattatggca ggagtgaggg
    14641 aggatgactg cgagtgggag cacggttttc accctcttcc cagagaggcg aggggagcgt
    14701 ccgagcatca tgaggccgaa aactctgctt ttcaggtaac ttattaccat aaactcagat
    14761 ttactgctgc ttcacgcagg atctgagttt atgggaatgc tcaacctgga agccggaggt
    14821 tttctgcaga ttcgcctgcc atgatgaagt tattcaagca agccaggaga tctgcatgat
    14881 gactgcaccc aaaattacat ttatcggcgc tggttcgacg attttcgtta aaaatattct
    14941 tggtgatgtg ttccatcgag aggcgctgaa aacggcgcat attgccctga tggacattga
    15001 tcccacccgc ctggaagagt cgcatattgt ggtgcgtaag ctgatggatt cagcaggggc
    15061 cagcggcaaa atcacctgcc acacccaaca gaaagaagcc ttacaggatg ccgattttgt
    15121 cgtggtggca tttcagattg gcggttatga accttgcacg gtgactgatt tcgaggtctg
    15181 taagcggcat ggtctggaac aaaccattgc cgatacgttg gggccgggcg gtattatgcg
    15241 cgcgctacgt accattccgc atctgtggca aatttgcgag gacatgacgg aagtctgccc
    15301 cgatgccaca ttgctcaact acgttaaccc aatggcgatg aatacctggg cgatgtatgc
    15361 ccgctatccg catatcaaac aggtagggct gtgccattcg gtgcagggaa cggcggaaga
    15421 gctggcgcgt gacctcaata tcgatccgtc cacgctgcgt taccgttgcg ctggtatcaa
    15481 ccatatggcg ttttacctgg aactggagcg taaaaccgcc gacggcagtt acgtgaatct
    15541 ctacccggaa ctgctggcgg cttatgaagc agggcaggca ccgaagccac atattcatgg
    15601 taatactcgc tgccagaata ttgtgcgcta cgaaatgttc aaaaagctgg gctacttcgt
    15661 cacggaatcg tcagaacatt ttgccgaata tacgccgtgg tttattaagc caggtcgtga
    15721 ggatttgatt gagcgttata aagtaccgct ggatgagtac ccgaaacgct gcgtcgagca
    15781 gctggcgaac tggcataaag agctggagga gtataaaaac gcctcccgga ttgatattaa
    15841 accgtcacgg gaatatgcca gcacaatcat gaacgctatc tggactggcg agtcgagtgt
    15901 gatttacggc aacgtccgta acgatggttt gattgataac ctgccacaag gatgttgcgt
    15961 ggaagtagcc tgtctggttg atgctaatgg cattcagccg accaaagtcg gtacgctacc
    16021 ttcgcatctg gccgccctga tgcaaaccaa catcaacgta cagacgctgc tgaccgaagc
    16081 tattcttacg gaaaatcgcg accgtgttta ccacgccgcg atgatggacc cgcatactgc
    16141 cgccgtgctg ggcattgacg aaatatatgc tcttgttgac gacctgattg ccgcccacgg
    16201 cgactggctg ccaggctggt tgcaccgtta aaacgcgact aaacgctact gcgccggggg
    16261 atttattccg gcgcacacct ctgacgatac caataacaga aggcgggcgt tggtaacagc
    16321 gacccgatac cctatgagca tttcaatgac tacaaaactc agttatggat ttggagcgtt
    16381 cgggaaggat tttgcgatcg gcattgtgta tatgtacctc atgtattact acaccgatgt
    16441 cgttgggctg tctgtgggtt tggtcggtac tttgtttctg gtggcgagga tctgggatgc
    16501 tattaacgat ccgattatg
//
LOCUS       DADLFA010000089        16472 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000089 DADLFA010000000
VERSION     DADLFA010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16472
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.089"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(118..1401)
                     /gene="gltA"
                     /locus_tag="JQH65_002392"
     CDS             complement(118..1401)
                     /gene="gltA"
                     /locus_tag="JQH65_002392"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HAY6139779.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(1607..1714)
                     /locus_tag="JQH65_002393"
     CDS             complement(1607..1714)
                     /locus_tag="JQH65_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6139780.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2095..2499
                     /gene="sdhC"
                     /locus_tag="JQH65_002394"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2095..2499
                     /gene="sdhC"
                     /locus_tag="JQH65_002394"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HAY6139781.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2493..2840
                     /gene="sdhD"
                     /locus_tag="JQH65_002395"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2493..2840
                     /gene="sdhD"
                     /locus_tag="JQH65_002395"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HAY6139782.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2840..4606
                     /gene="sdhA"
                     /locus_tag="JQH65_002396"
     CDS             2840..4606
                     /gene="sdhA"
                     /locus_tag="JQH65_002396"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HAY6139783.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4622..5338
                     /locus_tag="JQH65_002397"
     CDS             4622..5338
                     /locus_tag="JQH65_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HAY6139784.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLNDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5639..8440
                     /gene="sucA"
                     /locus_tag="JQH65_002398"
     CDS             5639..8440
                     /gene="sucA"
                     /locus_tag="JQH65_002398"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HAY6139785.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYSDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8455..9672
                     /gene="odhB"
                     /locus_tag="JQH65_002399"
     CDS             8455..9672
                     /gene="odhB"
                     /locus_tag="JQH65_002399"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HAY6139786.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKSPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLITIK
                     ELLEDPTRLLLDV"
     gene            9948..11114
                     /gene="sucC"
                     /locus_tag="JQH65_002400"
     CDS             9948..11114
                     /gene="sucC"
                     /locus_tag="JQH65_002400"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HAY6139787.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPQLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQLDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKVVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            11114..11983
                     /gene="sucD"
                     /locus_tag="JQH65_002401"
     CDS             11114..11983
                     /gene="sucD"
                     /locus_tag="JQH65_002401"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HAY6139788.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(12087..12809)
                     /gene="mngR"
                     /locus_tag="JQH65_002402"
     CDS             complement(12087..12809)
                     /gene="mngR"
                     /locus_tag="JQH65_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HAY6139789.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            12918..14894
                     /gene="mngA"
                     /locus_tag="JQH65_002403"
     CDS             12918..14894
                     /gene="mngA"
                     /locus_tag="JQH65_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HAY6139790.1"
                     /translation="MVLFYRAHWRDYKNDQVRIMMNLTTLTHRDALRLNARFTSREEA
                     IHALTQRLAALGKISSTEQFLEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVAT
                     LSEPLQWEGVDGPEAVDLVVLLAIPPNEAGTMHMQLLTALTTRLADDEIRARIQSATT
                     PDELLSALDDKGGTQPSASFSNAPTIVCVPACPAGIAHTYMAAEYLEKAGRKLGVNVY
                     VEKQGANGIEGRLTADQLNSATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEAL
                     IQQALTLKRSDETRTVQQDTQPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLL
                     SQIFGLQDLFNEENSWLWMYRKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGL
                     AANMIGSGFLGAVVGGLIAGYLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLML
                     FVVGEPVAWINNSLTAWLNGLSGSNALLLGAIFGFMCSFDLGGPVNKAAYAFCLGAMV
                     NGVYGPYAIFASVKMVSAFTVTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPM
                     AIEDPLRVIGSFVLGSMVTGAIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWF
                     GAALVGAAISTAILLIWRRHAVKHGNYLTDGVMP"
     gene            14912..>16472
                     /locus_tag="JQH65_002404"
     CDS             14912..>16472
                     /locus_tag="JQH65_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAY6139791.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPTDENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGSLQLV
                     DKDSGVRYDRVL"
BASE COUNT         3954 a         4135 c         4486 g         3897 t
ORIGIN      
        1 aatatatgaa tgtccgatag tggattttat ttttacaatt ttttgtgttt aggcatataa
       61 aaattaaccc gccatttgaa cggcgggtta aaatatttac aacttagcaa tcaaccatta
      121 acgcttgata tcgcttttaa agtcgcgttt ttcatatcct gtatacagct gacgcggacg
      181 ggcaatcttc ataccgtcac tgtgcatttc gctccagtgg gcgatccagc caacggtacg
      241 tgccattgcg aaaatgacgg tgaacatgga agacggaata cccatcgctt tcaggatgat
      301 accagagtag aaatcgacgt tcgggtacag tttcttctcg ataaagtacg ggtcgttcag
      361 cgcgatgttt tccagctcca tagccacttc cagcaggtca tctttggtgc ccagctcttt
      421 cagaacttca tggcaggttt cacgcattac ggtggcgcgc gggtcgtaat ttttgtacac
      481 gcggtgaccg aagcccatca ggcggaaaga atcattttta tctttcgcac gacgaacaaa
      541 ttccggaatg tgtttaacgg agctaatttc ttccagcatt ttcagcgccg cttcgttagc
      601 accaccgtgc gcaggtcccc acagtgaagc aatacctgct gcgatacagg caaacgggtt
      661 cgcacccgaa gagccagcgg tacgcacggt ggaggtagag gcgttctgtt catggtcagc
      721 gtgcaggatc agaatacggt ccatagcacg ttccagaatc ggattaactt catacggttc
      781 gcacggcgtg gagaacatca tattcaggaa gttaccggca taggagagat cgttgcgcgg
      841 ataaacaaat ggctgaccaa tggaatactt gtaacacatc gcggccatgg tcggcatttt
      901 cgacagcagg cggaacgcgg caatttcacg atgacgagga ttgttaacat ccagcgagtc
      961 gtgatagaac gccgccagcg cgccggtaat accacacatg actgccattg gatgtgagtc
     1021 gcgacggaaa gcgtggaaca gacgggtaat ctgctcgtgg atcatggtat gacgggtcac
     1081 cgtagtttta aattcgtcat actgttcctg agtcggtttt tcaccattca gcaggatgta
     1141 acaaacttcc aggtagttag aatcggtcgc cagctgatcg atcgggaaac cgcggtgcag
     1201 caaaatacct tcatcaccat caataaaagt aattttagat tcgcaggatg cggttgaagt
     1261 gaagcctggg tcaaaggtga acacaccttt tgaaccgaga gtacggatat caataacatc
     1321 ttgacccagc gtgcctttca gcacatccag ttcaacagct gtgtccccgt tgagggtgag
     1381 ttttgctttt gtatcagcca tttaaggtct ccttagcgcc ttattgcgta agactgccgg
     1441 aacttaaatt tgccttcgca catcaacctg gctttacccg tttgttattt ggctcgccgc
     1501 tctgtgaaag aggggaaaac ctgggtacag agctctgggc gcttgcaggt aaaggatcca
     1561 ttgatgacga ataaatggcg aatcaagtac ttagcaatcc gaattattaa acttgtctac
     1621 cactaataac tgtcccgaat gaattggtca atacttccac actgttacat aagttaatct
     1681 taggtgaaat accgacttca taacttttac gcattatatg cttttcctgg taatgtttgt
     1741 aacaactttg ttgagtgatt gtcaaattag atgattaaaa attaaataaa tgttgttgtc
     1801 gtgacctgga tcactgttca ggataaaacc cgacaaacta tatgtaggtt aattgtaatg
     1861 attttgtgaa cagcctatac tgccgccagg tctccggaac accctgcaat cccgagccac
     1921 ccagcgttgt aacgtgtcgt tttcgcatct ggaagcagtg ttttgcatga cgcgcagtta
     1981 tagaaaggac gctgtctgac ccgcaagcag accggaggaa ggaaatcccg acgtctccag
     2041 gtaacagaaa gttaacctct gtgcccgtag tccccaggga ataataagaa cagcatgtgg
     2101 gcgttattca tgataagaaa tgtgaaaaaa caaagacctg ttaatctgga cctacagacc
     2161 atccggttcc ccatcacggc gatagcgtcc attctccatc gcgtttccgg tgtgatcacc
     2221 tttgttgcag tgggcatcct gctgtggctt ctgggtacca gcctctcttc ccctgaaggt
     2281 ttcgagcaag cttccgcgat tatgggcagc ttcttcgtca aatttatcat gtggggcatc
     2341 cttaccgctc tggcgtatca cgtcgtcgta ggtattcgcc acatgatgat ggattttggc
     2401 tatctggaag aaacattcga agcgggtaaa cgctccgcca aaatctcctt tgttattact
     2461 gtcgtgcttt cacttctcgc aggagtcctc gtatggtaag caacgcctcc gcattaggac
     2521 gcaatggcgt acatgatttc atcctcgttc gcgctaccgc tatcgtcctg acgctctaca
     2581 tcatttatat ggtcggtttt ttcgccacca gtggcgaact gacatatgaa gtctggatcg
     2641 gtttcttcgc ctctgcgttc accaaagtgt tcaccctgct ggcgctgttt tctatcttga
     2701 ttcatgcctg gatcggcatg tggcaggtgt tgaccgacta cgttaaaccg ctggcattgc
     2761 gcctgatgct gcaactggtg attgtcgttg cactggtggt ttacgtgatt tatggattcg
     2821 ttgtggtgtg gggtgtgtga tgaaattgcc agtcagagaa tttgatgcag ttgtgattgg
     2881 tgccggtggc gcaggtatgc gcgcggcgct gcaaatttcc cagagcggcc agacctgtgc
     2941 gctgctctct aaagtcttcc cgacccgttc ccataccgtt tctgcgcaag gcggcattac
     3001 cgttgcgctg ggtaataccc atgaagataa ctgggaatgg catatgtacg acaccgtaaa
     3061 agggtcggac tatatcggtg accaggacgc gattgaatat atgtgtaaaa ccgggccgga
     3121 agcgattctg gaactcgaac acatgggcct gccgttctcg cgtcttgatg atggtcgtat
     3181 ctatcaacgt ccgtttggcg gtcagtcgaa aaacttcggc ggcgagcagg cggcacgcac
     3241 tgcggcggcg gctgaccgta ccggtcacgc actgttgcac acgctttatc agcagaacct
     3301 gaaaaaccac accaccattt tctccgagtg gtatgcgctg gatctggtga agaaccagga
     3361 tggcgcggtg gtgggttgta ctgcactgtg catcgaaact ggtgaagtgg tttatttcaa
     3421 agcccgcgct accgtgctgg cgactggcgg ggcagggcgt atttatcagt ccaccactaa
     3481 cgcccacatt aacactggcg acggcgtcgg catggctatc cgcgcaggcg taccggtgca
     3541 ggatatggaa atgtggcagt tccacccgac cggtattgcc ggtgcgggcg tactggtcac
     3601 cgaaggttgc cgtggtgaag gcggttatct gctgaacaaa catggcgaac gctttatgga
     3661 gcgttatgcg ccgaacgcca aagacctggc gggccgtgat gtggtggcgc gttccatcat
     3721 gatcgaaatc cgtgaaggtc gcggctgtga tggtccgtgg gggccacacg cgaaactgaa
     3781 actcgaccac ctgggtaaag aagttctcga atcccgtctg ccgggcatcc tggaactctc
     3841 ccgtaccttc gctcacgtcg atccggtgaa agagccgatc ccggttatcc caacctgtca
     3901 ttacatgatg ggcggtattc cgaccaaagt gaccggtcag gcgctgactg tgaatgagaa
     3961 aggcgaagat gtggttgttc cgggactgtt tgccgttggt gaaatcgctt gtgtatcggt
     4021 acacggcgct aaccgtctgg gcggcaactc gctgctggac ctggtggtct ttggtcgtgc
     4081 ggcaggtctg catctgcaag agtctatcgc cgagcagggc gcattgcgcg atgccagcga
     4141 gtctgatgtt gaagcatctc tggatcgtct gaaccgctgg aacaataatc gtaacggtga
     4201 agatccggtg gcgattcgta aagcgctgca agaatgtatg cagcataact tctcggtctt
     4261 ccgtgaaggt gatgcgatgg cgaaagggct tgagcagttg aaagttatcc gcgagcgtct
     4321 gaaaaatgcc cgtctggatg acacttccag cgagttcaac acccagcgcg ttgagtgcct
     4381 ggaactggat aacctgatgg aaacggcgta tgcaacggct gtttctgcca acttccgtac
     4441 cgaaagccgt ggcgcgcata gccgcttcga cttcccggat cgtgatgatg aaaactggct
     4501 gtgccactcc ctgtatctgc cagagtcgga atccatgacg cgccgaagcg tcaacatgga
     4561 accgaaactg cgcccggcat tcccgccgaa gattcgtact tactaatgcg gagacaggaa
     4621 aatgagactc gagttttcaa tttatcgcta taacccggat gttgatgatg ctccgcgtat
     4681 gcaggattac accctggaag cggaagaagg tcgcgacatg atgctgctgg atgcgcttat
     4741 ccagctgaaa gagaaagatc ccagcctgtc gttccgccgc tcctgtcgtg aaggtgtgtg
     4801 cggttccgac ggtctgaaca tgaacggcaa gaatggtctg gcctgtatta ccccgatttc
     4861 ggcactcaac cagccgggca agaagattgt gattcgcccg ctgccaggtt taccggtgat
     4921 ccgcgatttg gtggtagaca tgggacaatt ctatgcgcaa tatgagaaaa ttaagcctta
     4981 cctgttgaat aatggacaaa atccgccagc tcgcgagcat ttacagatgc cagagcagcg
     5041 cgaaaaactc gacgggctgt atgaatgtat tctctgcgca tgttgttcaa cctcttgtcc
     5101 gtctttctgg tggaatcccg ataagtttat cggcccggca ggcttgttag cggcatatcg
     5161 tttcctgaac gatagccgtg ataccgagac tgacagccgc ctcgacggtt tgagcgatgc
     5221 attcagcgta ttccgctgtc acagcatcat gaactgcgtc agtgtatgtc cgaaggggct
     5281 gaacccgacg cgcgccatcg gccatatcaa gtcgatgttg ttgcaacgta atgcgtaaac
     5341 cgtaggcctg ataagacgcg caagcgtcgc atcaggcaac cagtgccgga tgcggcgtga
     5401 acgccttatc cggcctacaa gtcattaccc gtaggcctga taagcgcagc gcatcaggcg
     5461 taacaaagaa atgcaggaaa tctttaaaaa ctgcccctga cactaagaca gtttttaaag
     5521 gttccttcgc gagccactac gtagacaaga gctcgcaagt gaaccccggc acgcacatca
     5581 ctgtgcgtgg tagtatccac ggcgaagtaa gcataaaaaa gatgcttaag ggatcacgat
     5641 gcagaacagc gctttgaaag cctggttgga ctcttcttac ctctctggcg caaaccagag
     5701 ctggatagaa cagctctatg aagacttctt aaccgatcct gactcggttg acgctaactg
     5761 gcgttcgacg ttccagcagt tacctggtac gggagtcaaa ccggatcaat tccactctca
     5821 aacgcgtgaa tatttccgcc gcctggcgaa agacgcttca cgttactctt caacgatctc
     5881 cgaccctgac accaatgtga agcaggttaa agtcctgcag ctcattaacg cataccgctt
     5941 ccgtggtcac cagcatgcga atctcgatcc gctgggactg tggcagcaag ataaagtggc
     6001 cgatctggat ccgtctttcc acgatctgac cgaagcagac ttccaggaaa ccttcaacgt
     6061 cggttcattt gccagcggca aagaaaccat gaaactcggc gagctgctgg aagccctcaa
     6121 gcaaacctac tgcggcccga ttggtgccga gtatatgcac atcaccagca ccgaagaaaa
     6181 acgctggatc caacagcgta tcgagtctgg tcgcgcgact ttcaatagcg aagagaaaaa
     6241 acgcttctta agcgaactga ccgccgctga aggccttgaa cgttacctcg gcgcaaaatt
     6301 ccctggcgca aaacgcttct cgctggaagg cggtgacgcg ttaatcccga tgcttaaaga
     6361 gatgatccgc cacgctggca acagcggcac ccgcgaagtg gttctcggga tggcgcaccg
     6421 tggtcgtctg aacgtgctgg tgaacgtgct gggtaaaaaa ccgcaagact tgttcgacga
     6481 gttcgccggt aaacataaag aacacctcgg cacgggtgac gtgaaatacc acatgggctt
     6541 ctcgtctgac ttccagaccg atggcggcct ggtgcacctg gcgctggcgt ttaacccgtc
     6601 tcaccttgag attgtaagcc cggtagttat cggttctgtt cgtgcccgtc tggacagact
     6661 tgatgagccg agcagcaaca aagtgctgcc aatcaccatc cacggtgacg ccgctgtgac
     6721 cgggcagggc gtggttcagg aaaccctgaa catgtcgaaa gcgcgtggtt atgaagttgg
     6781 cggtacggta cgtatcgtta tcaacaacca ggttggtttc accacctcta atccgctgga
     6841 tgcccgttct acgccgtact gtactgatat cggtaagatg gttcaggccc cgattttcca
     6901 cgttaacgcg gacgatccgg aagccgttgc ctttgtgacc cgtctggcgc tcgatttccg
     6961 taacaccttt aaacgtgatg tcttcatcga cctggtgtgc taccgccgtc acggccacaa
     7021 cgaagccgac gagccgagcg caacccagcc gctgatgtat cagaaaatca aaaaacatcc
     7081 gacaccgcgc aaaatctacg ctgacaagct ggagcaggaa aaagtggcga cgctggaaga
     7141 tgccaccgag atggttaacc tgtaccgcga tgcgctggat gctggcgatt gcgtagtggc
     7201 agagtggcgt ccgatgaaca tgcactcttt cacctggtcg ccgtacctca accacgaatg
     7261 ggacgaagag tacccgaaca aagttgagat gaagcgcctg caggagctgg cgaaacgcat
     7321 cagcacggtg ccggaagcag ttgaaatgca gtctcgcgtt gccaagattt atagcgatcg
     7381 ccaggcgatg gctgccggtg agaaactgtt cgactggggc ggtgcggaaa acctcgctta
     7441 cgccacgctg gttgacgaag gcattccggt tcgcctgtcg ggtgaagact ccggtcgcgg
     7501 taccttcttc caccgccacg cggtgatcca caaccagtct aacggttcca cttacacgcc
     7561 gctgcaacac atccataacg ggcagggcgc gttccgcgtc tgggactccg tactgtctga
     7621 agaagcagtg ctggcgtttg aatacggtta tgccaccgca gaaccacgca ctctgaccat
     7681 ctgggaagcg cagttcggtg acttcgctaa cggtgctcag gtggttatcg accagttcat
     7741 ctcctctggc gaacagaaat ggggccggat gtgtggtctg gtgatgttgc tgccgcacgg
     7801 ttatgaaggg caggggccgg agcactcctc cgcgcgtctg gaacgttatc tgcaactttg
     7861 tgctgagcaa aacatgcagg tgtgcgtacc gtctaccccg gcacaggttt accacatgct
     7921 gcgtcgtcag gcgctgcgcg ggatgcgtcg tccgctggtc gtgatgtcgc cgaaatccct
     7981 gctgcgtcat ccgctggcgg tttccagcct cgaagaactg gcgaacggca ccttcctgcc
     8041 agccatcggt gaaatcgacg agcttgatcc gaagggcgtg aagcgcgtag tgatgtgttc
     8101 tggtaaggtt tattacgacc tgctggaaca gcgtcgtaag aacaatcaac acgatgtcgc
     8161 cattgtgcgt atcgagcaac tctacccgtt cccgcataaa gcgatgcagg aagtgttgca
     8221 gcagtttgct cacgtcaagg attttgtctg gtgccaggaa gagccgctca accagggcgc
     8281 atggtactgc agccagcatc atttccgtga agtgattccg tttggggctt ctctgcgtta
     8341 tgcaggccgc ccggcctccg cctctccggc ggtagggtat atgtccgttc accagaaaca
     8401 gcaacaagat ctggttaatg acgcgctgaa cgtcgaataa ataaaggata cacaatgagt
     8461 agcgtagata ttctggtccc tgacctgcct gaatccgtag ccgatgccac cgtcgcaacc
     8521 tggcataaaa aacccggcga cgcagtcgta cgtgatgaag tgctggtaga aatcgaaact
     8581 gacaaagtgg tactggaagt accggcatca gcagacggca ttctggatgc ggttctggaa
     8641 gatgaaggta caaccgtaac gtctcgtcag atccttggtc gcctgcgtga aggcaacagc
     8701 gccggtaaag aaaccagcgc caaatctgaa gagaaagcgt ccactccggc gcaacgccag
     8761 caggcgtctc tggaagagca aaacaacgat gcgttaagcc cggcgatccg tcgcttgctg
     8821 gctgaacata acctcgacgc cagcgctatt aaaggcaccg gcgtgggtgg tcgtctgacc
     8881 cgtgaagatg tggaaaaaca tctggcgaaa tccccggcga aagagtctgc accggcagcg
     8941 gctgctccgg cggcgcaacc ggctctggct gcacgtagtg aaaaacgtgt cccgatgact
     9001 cgcctgcgta agcgtgtggc agagcgtctg ctggaagcga aaaactccac cgccatgctg
     9061 accacgttca acgaagtcaa catgaagccg attatggatc tgcgtaagca gtacggtgaa
     9121 gcgtttgaaa aacgccacgg catccgtctg ggctttatgt ccttctacgt gaaagcggtg
     9181 gttgaagccc tgaaacgtta cccggaagtg aatgcttcta tcgacggcga tgacgtggtt
     9241 taccacaact atttcgacgt cagcatggcg gtttctacgc cgcgcggcct ggtgacgccg
     9301 gttctgcgtg atgtcgatac cctcggcatg gcagacatcg agaagaaaat caaagagctg
     9361 gcagtcaaag gccgtgatgg caagctgacc gttgaagatc tgaccggtgg taacttcacc
     9421 atcaccaacg gtggtgtgtt cggttccctg atgtctacgc cgatcatcaa cccgccgcag
     9481 agcgcaattc tgggtatgca cgctatcaaa gatcgtccga tggcggtgaa tggtcaggtt
     9541 gagatcctgc cgatgatgta cctggcgctg tcctacgatc accgtctgat cgatggtcgc
     9601 gaatccgtgg gcttcctgat aacgatcaaa gagttgctgg aagatccgac gcgtctgctg
     9661 ctggacgtgt agtagtttaa gtttcacctg cactgtagac aggataaggc attatcgcct
     9721 tctccggcaa ttgaagcctg atgcgacgct taacgcgtct tatcaggcct acgggaagca
     9781 ccaatgtagg tcggataagg cgcttgcgcc gcatccgaca agcgatgcct gatgcgacac
     9841 ttacgcgtct tatcaggcct acaggtgacc gacaatgccc ggaggcgata cgaaatattc
     9901 ggtctacggt ttaaaagata acgattactg aaggatggac agaacacatg aacttacatg
     9961 aatatcaggc aaaacaactt tttgcccgct atggcttacc agcaccggtg ggttatgcct
    10021 gtactactcc gcgcgaagca gaagaagccg cttcaaaaat cggtgccggt ccgtgggtag
    10081 tgaaatgtca ggttcacgct ggtggccgcg gtaaagcggg cggtgtgaaa gttgtaaaca
    10141 gcaaagaaga catccgtgct tttgcagaaa attggctcgg caagcgtctg gtaacgtatc
    10201 aaacagatgc caatggccaa ccggttaacc agattctggt tgaagcagcg accgatatcg
    10261 ctaaagagct gtatctcggt gccgttgtag accgtagttc ccgtcgtgtg gtctttatgg
    10321 cctccactga aggcggcgtg gaaatcgaaa aagtggcgga agaaactccg cagctgatcc
    10381 ataaagttgc gcttgatccg ctgacaggcc cgatgccgta tcagggacgc gagctggcgt
    10441 tcaaactggg tctggaaggt aaactggttc agcagttcac caaaatcttc atgggtctgg
    10501 cgaccatttt cctggagcgc gacctggcgt tgatcgaaat caacccgctg gtcatcacca
    10561 aacagggtga tctgatttgc ctcgacggca aactgggcgc tgatggcaac gcactgttcc
    10621 gccagcttga tctgcgcgaa atgcgtgacc agtcgcagga agatccgcgt gaagcacagg
    10681 ctgcacagtg ggaactgaac tacgttgcgc tggacggtaa catcggttgt atggttaacg
    10741 gcgcaggtct ggcgatgggt acgatggaca tcgttaaact gcacggcggc gaaccggcta
    10801 acttccttga cgttggcggc ggtgcaacca aagaacgtgt aaccgaagcg ttcaaaatca
    10861 tcctctctga cgacaaagtg aaagtcgttc tggttaacat cttcggcggt atcgttcgtt
    10921 gcgacctgat cgctgacggt atcatcggcg cggtagcaga agtgggtgtt aacgtaccgg
    10981 tcgtggtacg tctggaaggt aacaacgccg aactcggcgc gaagaaactg gctgacagcg
    11041 gcctgaatat tattgcagca aaaggtctga cggatgcagc tcagcaggtt gttgccgcag
    11101 tggaggggaa ataatgtcca ttttaattga taaaaacacc aaggttatct gccagggctt
    11161 tactggtagc caggggactt tccactcaga acaggccatt gcatacggca ctaaaatggt
    11221 tggcggcgta accccaggta aaggcggtac tacccacctc ggcctgccgg tgttcaacac
    11281 cgtgcgtgaa gctgttgctg ctactggcgc taccgcttct gttatctacg taccagcacc
    11341 gttctgcaaa gactccattc tggaagctat tgatgcaggc atcaaactga ttatcaccat
    11401 cactgaaggc atcccgacgc tggatatgct gactgtgaaa gtgaaactgg acgaagccgg
    11461 cgttcgtatg atcggcccga actgcccggg cgttatcact ccgggtgaat gcaaaatcgg
    11521 tatccagccg ggtcacattc acaaaccggg taaagtgggt atcgtttccc gttccggtac
    11581 actgacctat gaagcggtta aacagaccac ggattacggt ttcggtcagt cgacctgtgt
    11641 tggtatcggc ggtgacccga tcccaggctc taactttatc gacatcctcg aaatgttcga
    11701 aaaagatccg cagaccgaag cgatcgtgat gatcggtgag atcggcggta gcgctgaaga
    11761 agaagcggca gcgtacatca aagagcacgt taccaagcca gttgtaggtt acatcgctgg
    11821 tgtgactgcg ccgaaaggca aacgtatggg ccacgcgggt gcgatcattg ccggtgggaa
    11881 agggactgcg gatgagaaat tcgctgctct ggaagccgca ggcgtgaaaa ccgttcgcag
    11941 cctggcggat atcggtgaag cactgaaaac tgttctgaaa taaatatctg taataagaaa
    12001 tagccctcgc cgcttccctc tacaggaatg gcgaagggct gtcggtttcg acatggttgg
    12061 ccatcgtatg atggcctttt ttgtgcttat cgcgatgatt ttcgctgcgc tatcagggta
    12121 aatttatagt catcggtatt aaaagcgttg cggctatatt caaacacccg accatcaact
    12181 aaatatccac gcgatacttt ttcaagaatc ggctttgtct ggctgatatt aagcagacgg
    12241 ctcatctctt cggttggcat cagaggaatg atttcctgtt cgctacgatc gataaccatc
    12301 ttcttcactt cttcaataaa gtgatatttc gaattttcca tgacctgcca ggtgagatcc
    12361 gggaacaacg caagcggcat ccaggtttct tccagcgcca ttggcttttg cttgcgatag
    12421 cgcacgcgct tcacatgcca cacacgatcc tgcgcggtga tttgtagctg ttgctgaaga
    12481 aaatcgtcag ccggaatcac ttcaaatatc aggacttcac tgtgtgtatc gacgtgacgg
    12541 tccgacagtt tttcatcaaa actggttaac tgaaaaatat cgtaattgac ccgctcttct
    12601 ttgacgtaag tcccgctgcc ctgaatgctt tcgaggatct gctgctcgac tagctggcgc
    12661 aaagcctgac gcaccgtaac ccggctgacg ccaaactctg tttgtagcgc tgattcagtg
    12721 ggtaacgcat cgccaggttt aagctcgcca cgcgcaattt gttcacgaat gcgatcggca
    12781 atctgccggt ataagggctt gtgtcccatt tttagtatct cattaatacg aatttagcca
    12841 ttatgcccga taaattcatc ctgtaaataa tgcaaataca atacaaataa tttcaatcaa
    12901 gtgaaattga tcatataatg gtattgtttt atcgggcaca ctggcgcgac tataaaaacg
    12961 atcaagtgag gatcatgatg aatctgacga ctctgaccca ccgcgatgcg ttgcgtctga
    13021 atgcgcgctt taccagccgt gaagaggcca tccacgcgtt gactcaacgt cttgctgctc
    13081 tggggaaaat ttccagtact gagcaatttc tggaagaagt gtatcgccgt gaaagccttg
    13141 gcccgaccgc cttaggtgaa gggttggctg tgccgcatgg caaaactgct gcggtaaaag
    13201 aagcggcgtt tgcggtcgcc acactcagcg agccgcttca gtgggaaggc gttgatggcc
    13261 cggaagcagt tgatttagtg gtgctgctgg cgattccccc caatgaagcg ggtactatgc
    13321 atatgcaact gctgacagcg ctgaccacgc gccttgcgga tgatgagatt cgggcgcgta
    13381 tacagtcggc gacgacgcct gatgagttgc tctcggcgct ggatgacaag ggaggcacgc
    13441 aaccttctgc ctctttttcc aacgcgccaa ctatcgtctg cgtaccggcc tgtccggcgg
    13501 gtattgctca cacctatatg gctgcggaat atctggaaaa agccggacgc aaactcggcg
    13561 taaatgttta cgttgaaaaa caaggcgcta acggcattga agggcgttta acggcggatc
    13621 aactcaatag tgcaaccgcc tgtatttttg cggctgaagt cgccatcaag gagagtgagc
    13681 gttttaatgg cattcccgcg ctttcagtgc ctgttgccga gccgattcgc catgcagaag
    13741 cgttgatcca acaagcgctt accctcaagc gtagcgatga gacgcgtacc gtacagcaag
    13801 atacgcaacc ggtgaaaagt gtcaaaacgg agctgaaaca ggcactgttg agcggaatct
    13861 cttttgccgt accgttgatt gtcgcggggg gcacggtgct ggcggtcgcg gtattactgt
    13921 cgcaaatctt cgggctacaa gatctgttta atgaagaaaa ctcctggctg tggatgtacc
    13981 gcaagctggg cggcgggctg ctcggaattt tgatggtacc ggtgctcgcg gcctataccg
    14041 cctattctct ggcagataaa ccggcgttag cgccaggctt tgcggctgga cttgccgcca
    14101 acatgatcgg ctccgggttt ctcggcgcgg tcgttggcgg attgatagcc ggttacttga
    14161 tgcgctgggt gaaaaatcac ttgcgtctta gcagtaaatt caatggattc ctgacttttt
    14221 atctctaccc ggtgctcggt acgttgggag cgggcagtct gatgctgttt gtggtgggag
    14281 aacctgtcgc atggatcaat aactcgctta ccgcctggct gaacggtctg tcaggaagta
    14341 acgcgctgtt gctgggtgcc attttcggtt ttatgtgttc ctttgatctt ggagggccag
    14401 tgaacaaagc cgcttatgca ttctgcctgg gcgcaatggt gaacggcgtt tacggcccgt
    14461 atgccatttt cgcctccgtc aaaatggttt cggcatttac cgtaaccgct tccacgatgc
    14521 tcgcaccgcg cctgtttaaa gagtttgaaa ttgagaccgg gaaatccacc tggctgttag
    14581 ggctggcagg tattaccgaa ggggcgatcc cgatggcgat tgaagatccg ctgcgggtta
    14641 ttggttcgtt tgtgctgggc tctatggtaa cgggcgctat tgtcggtgcg atgaatatcg
    14701 gcctttcgac acccggtgcc ggcattttct cgctcttttt acttcatgat aatggcgcgg
    14761 gcggtgttat ggcggcaatt ggctggtttg gtgcggcatt ggtgggggct gcaatctcga
    14821 ctgcaattct cctgatctgg cggcgtcacg cggttaagca tggcaactat ctgactgatg
    14881 gcgtaatgcc ataaacaaaa aggaaacgac gatgaaagcg gtatctcgcg ttcacatcac
    14941 cccgcatatg cactgggatc gagagtggta tttcaccacc gaagagtcac gtattctgct
    15001 ggtcaataat atggaagaga tcctgtgccg actggaacag gacaacgaat acaaatatta
    15061 cgtactcgac gggcaaacgg cgatcctcga agattatttc gcggtgaaac cggaaaacaa
    15121 agaccgtgtg aagaaacagg tagaagccgg caagttgatt atcggcccct ggtataccca
    15181 gaccgatacc acgattgttt ctgcggaatc catcgtccgt aatctgatgt acggaatgcg
    15241 tgactgcctc gcgtttggcg agccgatgaa aataggttat ttaccagatt cctttggcat
    15301 gtccgggcaa ctgccgcata tctacaatgg atttggcatt acccgcacca tgttctggcg
    15361 cggatgttcg gagcgccacg gtactgataa aaccgagttt ttgtggcaaa gcagtgacgg
    15421 tagcgaagtg acggcgcagg tgctgccgct gggctacgcc ataggtaagt acttacctac
    15481 cgacgaaaac ggattacgta aacgcctcga cagttatttt gacgtgctgg aaaaagcgtc
    15541 tgtaaccaaa gagattttgc tgccgaatgg tcatgaccag atgccattgc agcaaaatat
    15601 cttcgaagtg atggataagc tacgtgagat ctaccctcaa cgtaagtttg tgatgagccg
    15661 ctttgaagag gtatttgaga agatcgaagc gcagcgtgaa agtctggcca ccctgaaagg
    15721 tgaatttatc gatggcaaat atatgcgcgt acatcgcacc atcggttcca cgcgcatgga
    15781 tatcaaaatt gcccacgcac gtattgaaaa taagattgtt aatctgctgg aaccgctggc
    15841 aacgctggcc tggacgttgg gttttgaata ccaccacggc ttgctggaga aaatgtggaa
    15901 agagatctta aaaaatcatg cccacgacag tatcggttgt tgttgcagtg acaaagttca
    15961 tcgcgaaatt gtcgcccgct tcgaactggc tgaagacatg gcggataatc tgctgcgttt
    16021 ctacatgcgc aaaattgccg ataacatgcc acagagtgat gccgacaaac tcgtgctgtt
    16081 taacctgatg ccctggccgc gggaagaagt tattaacacc actgtgcggc tgcgcgccag
    16141 ccagtttaat ctacgagacg atcgcggtca gcctgtaccg tattttattc gccatgcccg
    16201 tgagatcgat ccaggcctaa tcgatcggca aatagttcat tacggtaatt acgatccctt
    16261 tatggagttt gatatacaga tcaaccagat tgtcccttct atgggctatc gcacgcttta
    16321 tatcgaagcg aatcagcctg gcaacgtgat tgcggcaaaa agtgacgctg aagggatact
    16381 ggaaaatgct ttctggcaaa ttgcgctcaa tgaggatggt tctctgcaac tggtagataa
    16441 agacagcggt gtgcgctatg accgagtatt gc
//
LOCUS       DADLFA010000090        16381 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000090 DADLFA010000000
VERSION     DADLFA010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16381
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.090"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..190
                     /locus_tag="JQH65_002405"
     CDS             <1..190
                     /locus_tag="JQH65_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6139792.1"
                     /translation="DMLNPTKDTNWNSTCIYKSRHKMLPVNLTQETLFNSKSQDKHAL
                     FPIFTASWRAYRIMNKGA"
     gene            413..910
                     /gene="ubiC"
                     /locus_tag="JQH65_002406"
     CDS             413..910
                     /gene="ubiC"
                     /locus_tag="JQH65_002406"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HAY6139793.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            923..1795
                     /gene="ubiA"
                     /locus_tag="JQH65_002407"
     CDS             923..1795
                     /gene="ubiA"
                     /locus_tag="JQH65_002407"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HAY6139794.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(1950..4373)
                     /gene="plsB"
                     /locus_tag="JQH65_002408"
     CDS             complement(1950..4373)
                     /gene="plsB"
                     /locus_tag="JQH65_002408"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HAY6139795.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPSHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4544..4912
                     /locus_tag="JQH65_002409"
     CDS             4544..4912
                     /locus_tag="JQH65_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAY6139796.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5022..5630
                     /gene="lexA"
                     /locus_tag="JQH65_002410"
     CDS             5022..5630
                     /gene="lexA"
                     /locus_tag="JQH65_002410"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HAY6139797.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5649..7028
                     /gene="dinF"
                     /locus_tag="JQH65_002411"
     CDS             5649..7028
                     /gene="dinF"
                     /locus_tag="JQH65_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709876.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HAY6139798.1"
                     /translation="MPPGVAVCFSSLFIRLVCMAFLTSSDKALWHLALPMIFSNITVP
                     LLGLVDTAVIGHLDSPVYLGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQA
                     LARALVQPLLLALGAGALIALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPAS
                     LANLVLLGWLLGVQYARAPVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYA
                     TLLIGLLMVRKILKLRGISGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVL
                     GARLGSDIIAVNAVLMTLLTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACR
                     QSGIVALLFSVVYLLAGEHIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMF
                     IGATRAAEMRNSMAVAAAGFALTLLTLPWLGNHALWLALTVFLALRGLSLAAIWRRHW
                     RNGTWFAAT"
     gene            7144..7353
                     /locus_tag="JQH65_002412"
     CDS             7144..7353
                     /locus_tag="JQH65_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAY6139799.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7395..7910)
                     /gene="zur"
                     /locus_tag="JQH65_002413"
     CDS             complement(7395..7910)
                     /gene="zur"
                     /locus_tag="JQH65_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HAY6139800.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQSTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            8228..8482
                     /locus_tag="JQH65_002414"
     CDS             8228..8482
                     /locus_tag="JQH65_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6139801.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFDNEDAIKDKLDNRGSIKGKLNNI
                     YGKSIDYAALRHRDIIIAKIDLFIQRITHNLWHARKKMCF"
     gene            8506..9213
                     /locus_tag="JQH65_002415"
     CDS             8506..9213
                     /locus_tag="JQH65_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HAY6139802.1"
                     /translation="MWVNKYIDDCTDEDLNDRDFIASVVDRAIFHFAINSICNPGDNK
                     DATPIEQCTFDVETKNGLPSTVQLFYEESKDNEPLANIHFQTIGSGFLTFVNACQEHD
                     DNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLSQRDMKKYLGEMKR
                     LADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDPTSKEEIKIL
                     TDTSKLIYDLFGVKSEK"
     gene            9576..10613
                     /gene="dusA"
                     /locus_tag="JQH65_002416"
     CDS             9576..10613
                     /gene="dusA"
                     /locus_tag="JQH65_002416"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HAY6139803.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECGMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDAYPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            10747..10989
                     /gene="pspG"
                     /locus_tag="JQH65_002417"
     CDS             10747..10989
                     /gene="pspG"
                     /locus_tag="JQH65_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HAY6139804.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(11154..12137)
                     /locus_tag="JQH65_002418"
     CDS             complement(11154..12137)
                     /locus_tag="JQH65_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HAY6139805.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGSGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            12220..13635
                     /locus_tag="JQH65_002419"
     CDS             12220..13635
                     /locus_tag="JQH65_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase DnaB"
                     /protein_id="HAY6139806.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            13688..14767
                     /gene="alr"
                     /locus_tag="JQH65_002420"
     CDS             13688..14767
                     /gene="alr"
                     /locus_tag="JQH65_002420"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAY6139807.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAGAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            15020..16213
                     /gene="tyrB"
                     /locus_tag="JQH65_002421"
     CDS             15020..16213
                     /gene="tyrB"
                     /locus_tag="JQH65_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HAY6139808.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNLGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         3915 a         3903 c         4336 g         4227 t
ORIGIN      
        1 tgatatgttg aatcctacaa aagataccaa ttggaattct acgtgtatct ataaatccag
       61 acataagatg ttgcctgtta atcttactca ggaaacactt ttcaactcca aatctcagga
      121 taaacatgcg cttttcccca tttttactgc gagttggcga gcttatcgta taatgaataa
      181 gggtgcttaa gtaaaggaaa acatcaccgt tcctggcatc ctgggcggtg atgcccctat
      241 ggttgccctc gccagcgcgg gcatcggcaa agcgtaaggt tcaaaagcga tttaccactt
      301 catgcgattg ttgcgttttt gttgcgcatt agatcactta atttgcttta catctcccgt
      361 aaacactttt ctgcgataca atgcctttac gttatgtaac ggagagttcg gcatgtcaca
      421 ccccgcgtta acgcaactgc gtgcgctgcg ctattttaaa gagatccctg ccctggatcc
      481 gcaactgctc gactggctgt tgctggagga ttccatgaca aaacgttttg aacagcaggg
      541 aaaaacggta agcgtgacga tgatccgcga agggtttgtc gagcagaatg aaatccccga
      601 agaactgccg ctgctgccga aagagtctcg ttactggtta cgtgaaattt tgttatgtgc
      661 cgatggtgaa ccgtggcttg ccggtcgtac cgtcgttcct gtgtcaacgt taagcgggcc
      721 ggagctggcg ttacaaaaat tgggtaaaac gccgttagga cgctatctgt tcacatcatc
      781 gacattaacc cgggacttta ttgagatagg ccgtgatgcc gggctgtggg ggcgacgttc
      841 ccgcctgcga ttaagcggta aaccgctgtt gttaacagaa ctgtttttac cggcgtcacc
      901 gttgtactaa gaggaaaaaa atatggagtg gagtctgacg cagaataagc tgctggcgtt
      961 tcatcgctta atgcgtacgg ataagccaat tggcgcgtta ctgctgctct ggccaacatt
     1021 atgggcgttg tgggtggcga caccgggcgt tcctcagctc tggatcctgg cggtatttgt
     1081 cgcgggtgtc tggctgatgc gcgctgccgg atgtgtggtg aatgattatg ctgaccgaaa
     1141 gtttgatggt catgttaagc gcacggcgaa ccgaccactt cccagcggcg cggtaacaga
     1201 gaaagaggcg cgcgcgctgt ttgtagtgct ggtactgatt tcgtttttac tggtactgac
     1261 gctgaatacg atgaccattc tgttgtcgat tgccgctctg gcgttggcgt gggtgtaccc
     1321 gtttatgaag cggtataccc atctcccgca agtggtgctg ggcgcggcgt ttggctggtc
     1381 gattccaatg gcttttgccg ctgtgagtga gtcggtgcca ttgagttgct ggttaatgtt
     1441 cctcgccaat attctctggg cggtggctta cgacacgcag tatgcgatgg ttgaccgcga
     1501 tgatgatgtg aagattggca ttaaatccac ggcaatcctg ttcggccaat acgataaatt
     1561 gattattggt attttgcaga ttggcgtact ggcactgatg gcgatcatcg gtgagttaaa
     1621 tggcttaggc tggggatatt actggtcaat tctggtggct ggcgcgctgt ttgtttatca
     1681 acaaaaactg attgccaacc gcgagcgtga agcctgcttt aaagcattta tgaataataa
     1741 ctatgttggt ctggtactat ttttagggct ggcaatgagt tactggcatt tctgatgatg
     1801 taaaaaagcc ggatgatcat ccggctttct tctgggttgc ctgatgcgcg gcgcttctca
     1861 ggcctacaca acacatcgca atttattgaa tttgcagatt atggaatgcc ggataaggcg
     1921 ttttcgccgc atccggcaat tctctctggt tacccttcgc cctgcgtcgc actctcaatc
     1981 gtcaaacgca cgtctgatgt aatcaactcc gccagcaact gataaacctt catcgtttct
     2041 gccggttcgg catcgccgct atcgctgata tacccttcat cacgcagcgt cagcaccaga
     2101 gaactgaaca ccgccttgtc gaagaactcc ggcgcgttga taccgtgcag cacggagaga
     2161 cgttgcgcga cggtgcggct ctctttctcc agcgtaccgc ggttgatcga cgggttagcg
     2221 ctcaacagcc agaaggtgat ggcataacgt tgcagcgttt cgcgcgcgcc tgcggccagc
     2281 agctgtagcg tgcgagaatg cgacgggttg atatgcaact catcatcttg cagggtaatc
     2341 agcccctgac gttgcatctc atttgccaac gcatcaataa cgtccggcaa ctcgtcgcga
     2401 tcccagcgca ggaacagctc cgctttcagc attgggtaaa gcacattgac gtgctccatc
     2461 aatacgtcgc gggagatgtg gcgatgctgg gtgacaattg ccgccatcag cgaaggcagc
     2521 accaacatat gcgcaatgtt gttgcgatag taggtcatca gcaccgcttg ctcgcgcggc
     2581 agaatgatga tgtcgccgat agtgtctttc tcaacttcaa acttgttcat ttgcagcgcg
     2641 tgatcgataa gctcgctggc gctggctgaa ggaacggtag agtccgtgga gtaaggtacg
     2701 ttgcgcatca gatccaggta gcagttgagt tgctcggtta actgctcgcg ggtaagtgag
     2761 cgctgacgtg atgccagtag cgcggtacag cagaggttca tggcgtttgc cgcgcctgcg
     2821 ttgttaatgc gtaccatcag atcggcagca atattattga ccgtcggcgt taaccatgcc
     2881 ggacgcaccg cttcgatggg atcgatagat tcacgccagt caggtacatg ctggttaagg
     2941 taggtcatca acggcattgg ttcaccgaag ttgacgtaac cctgaccgag attacgcagc
     3001 ttgcttaaac cgcgcagcat ctgcggcagg ctctctttct ctttcgtcgc accgcgcagt
     3061 tctttggcgt aagtacccac ttccatgacg tgctcataac cgatatagat cggaatcagc
     3121 gtaatcggac gcgtgccgcc acgcagcatc gcctgaatgg tcatcgacag cgtaccggtt
     3181 ttcggatcca gcaaacgccc cgtacgggaa cgaccgcctt ccacgaaata ctcgacggaa
     3241 taaccacggc tgaacagttc gccgagatac tcacggaaaa cggtggaata aagtttattg
     3301 cctttaaacg tacggcgaat aaagaacgcc cccagacggc ggaaaatcgg cccggccggc
     3361 cagaaattca ggttgatccc ggcggcgata tgcggcggca ccagcccctg gtggtacagc
     3421 acgtaagaaa gtagcaggta gtccatgtga ctgcggtggc aaggcacata caccagctca
     3481 tgaccgtcgt gggccagctg gcgaacgcgc tcggcgttat ggacgttgat gccctgataa
     3541 agtcggttcc aggtgaagcc cagaatgcgg tcagtcaggc ggatcatctc gtaagagaaa
     3601 ttcgccgcaa tctcttccat cagcgcaatc gcgttctgct gcgctttttc atgggagatt
     3661 tttttgctgc gcgcttcatc ttctaccgct ttggcaatgg cgcgggaggc gagcagctta
     3721 ttaaacagat cctgacgagc aggcagacgt gggcccacag cagccagacg ttgacgggca
     3781 aagtgcatac gcgccacgcg cgccagtttc tgtgcgatag ttttatccgt gccgtgttca
     3841 tccgccatac ggcgcagcga aactgacggc gagaaacgca caaaactgtc gcgaccgagc
     3901 cacagtacgg cgaaaaattt ctgtacgccg ttaagcatac gcagcggcgg gtttacttcg
     3961 cctttttcac gccccggcgc gcgaccaaac atcaccgata ctggcaccat ctgcacatcc
     4021 agatttgggt tgctacggtg caaatcgaga tagtcgtgga acagcttaat agactcttct
     4081 ttcggcgtgt aataggtgaa tacacgcggc ccgccgtgaa tgaacacata gcgcggcagt
     4141 agcgtgccgt cgatttccag cggctctaac gggtcaggca agtcatgagc cagacactgg
     4201 gcgcgtaacg tcagcaaatc tgctttcgag ttgtacggta aaacgtacat aattggacga
     4261 gaggtatcca gccccagttc cggggcagga tctgccggaa tagacttgct ttttaccagg
     4321 atgcttaatg gtaaattcag taatttgtag taaattcgtg gccagccgga cataaacgat
     4381 gtaaagcctc tggttaataa tgcaaatgcg cggcaaggat agcagaaagt catgggaaat
     4441 tctgtggtat ccgctcatgt ttcgcgcggc gttacgcaaa cccgaatcat cggatttaac
     4501 ggtacactga tattgacgct cataatgtaa aaaggttctt tcaatggcca ataataccac
     4561 tggattcacc cgaattatca aagctgctgg ctattcctgg aaaggtttac gcgctgcatg
     4621 gatcaacgaa gcggcattcc gtcaggaagg cgtagcggta ttgttggcgg tggtcatcgc
     4681 ctgctggctg gatgtggacg caattacccg cgtgctgctt atcagctccg tgatgctggt
     4741 gatgattgtg gaaatcctca atagcgccat cgaagcagtg gttgaccgaa ttggctctga
     4801 ataccatgag ctttccggac gcgcaaaaga tatgggatcc gccgcggtgc tgattgcgat
     4861 tatcgtcgcc gtgataacct ggtgcatcct gttatggtcg cattttggat aacccttcca
     4921 gaattcgata aatctctggt ttattgtgca gtttatggtt ccaaaatcgc cttttgctgt
     4981 atatactcac agtataactg tatatacacc cagggggcgg aatgaaagcg ttaacggcca
     5041 ggcaacaaga ggtgtttgat ctcatccgtg atcacatcag ccagacaggt atgccgccga
     5101 cgcgtgcgga aatcgcgcag cgtttggggt tccgttcccc aaacgcggct gaagaacatc
     5161 tgaaggcgct ggcacgcaaa ggagttattg aaattgtttc cggcgcatca cgcgggattc
     5221 gtctgttgca ggaagaggaa gaagggttgc cgctggtagg tcgtgtggct gccggtgaac
     5281 cgcttctggc gcagcagcat attgaaggtc attatcaggt cgatccttcc ttattcaagc
     5341 cgaatgctga tttcctgctg cgcgtcagcg ggatgtcgat gaaagatatt ggcattatgg
     5401 atggcgactt gctggcagtg cataaaactc aggatgtacg taacggtcag gtcgttgtcg
     5461 cacgtattga tgacgaagtt accgttaagc gcctgaaaaa acagggcaat aaagtcgaac
     5521 tgttgccaga aaatagcgag tttaaaccaa ttgtcgttga ccttcgtcag cagagcttca
     5581 ccattgaagg gctggcggtt ggagttattc gcaacggcga ctggctgtaa catatctctg
     5641 agaccgcgat gccgccaggc gtcgcggttt gtttttcatc tctcttcatc aggcttgtct
     5701 gcatggcatt cctcacttca tctgataaag cactctggca tctcgcctta cccatgattt
     5761 tctccaatat caccgttccg ttgctgggac tggtcgatac ggcggtaatt ggtcatcttg
     5821 atagtccagt ttatttgggc ggcgtggcgg ttggtgcaac ggcgaccagc tttctcttta
     5881 tgctgttgct gtttttacgc atgagcacca ccgggctgac tgcgcaggct tatggtgcca
     5941 aaaatcctca ggcattagcc cgtgcgctgg tgcaaccgtt gctgttggcg ttgggggcag
     6001 gggcgttaat tgcgctgctg cgtacaccga ttatcgatct ggcactgcat attgttggcg
     6061 gtagtgaagc agtcctggaa caggcgcggc gctttcttga aatccgctgg ttaagcgcac
     6121 cggcgtcact ggcgaatctg gtattacttg gttggttact cggcgtgcaa tatgcccgtg
     6181 cgccagtaat tttgttagtg gtcggcaata tcctcaacat tgtgctggat gtctggctgg
     6241 tgatggggct gcatatgaac gtgcagggcg cggcgctggc gacggttatt gcggaatatg
     6301 caacattgct gattggtctg ctaatggtgc gtaaaatcct caaactacgc ggaatttccg
     6361 gcgaaatgct gaaaactgcc tggcgaggaa acttccgtcg cttgctggcg cttaaccgcg
     6421 atatcatgct gcgttcgctg ttgttgcaac tctgtttcgg cgcgatcacc gtacttggtg
     6481 cgcgactggg gagtgacatt atcgctgtta acgcggttct gatgacgcta ctcaccttta
     6541 ccgcctatgc gctggatggt tttgcctacg cggttgaagc gcattccggt caggcgtacg
     6601 gtgcgcgcga cggtagccag ttgctggatg tctggcgggc agcgtgccgc cagtcgggga
     6661 tcgtagcgtt actgttttcg gtagtttatt tgctggcagg ggaacacatc attgcgttgc
     6721 tgacgtcgtt aacccagatt cagcagctgg ccgaccgcta tcttatctgg caggtgattt
     6781 tgccgttggt tggcgtctgg tgttatctgc tggacggcat gtttataggc gcaacgcgcg
     6841 cagccgaaat gcgtaacagt atggcggtgg ccgccgcagg ttttgcgctg acgctcctta
     6901 cgctgccgtg gctgggtaat catgctttgt ggctggcgtt aaccgtcttt ctggcgttgc
     6961 gcgggctttc tctggcggct atctggcggc gtcactggcg caacggtacc tggtttgccg
     7021 caacgtgacg gttaaaaatt ctgaataaat aatcctaagc caaattgctg actacactta
     7081 atctcacgtt cagaagaaaa gtgaacgtac tctcattcac aacctaacga tgaggtcttg
     7141 attatgaata aagatgaagc cggcggtaac tggaaacagt ttaaaggtaa agtgaaagag
     7201 caatggggca aactgaccga tgatgatatg acgatcattg aaggtaaacg tgatcaactg
     7261 gtcggtaaaa tccaggaacg ttatggttat cagaaagatc aggcagaaaa agaggtcgtg
     7321 gattgggaaa cccgcaatga atatcgctgg taattaatcc ctcctgcccg acgtgtacaa
     7381 ggatgtacgc cctcttaacg cggtttcttt ttcacctgca cagagtgatc atgctggcac
     7441 tgttcaggat gacgacacgc ttccacttcc acgcatgccg cacagagccc atgtgcttca
     7501 atcacattat gccgcagggc aaaccccatt tttgccgcca gcgtatgcat gatgtcttcc
     7561 acgccttctg cacactcttc tttcactgcg ccgcagcgat cgcaaataaa catagctgaa
     7621 gtatgggtgg actggtcgaa cagatgacag agcacataac tgttggtgga ttccacctta
     7681 tgcacaaaac cttgctcaag cagaaaatcc agcgcgcgat aaaccgttgg cggcttggct
     7741 tgcggttcag cttcgcgcag taaatcaagc agatcataag cgctgatagc gccatcctgc
     7801 agactcatca ggcgcaacac ttccaggcgc tgtggggtca ggcgcacatt acgctgcgcg
     7861 cagatttttt cagcctgcgc taataactcc tgcgttgtgg tcttttccat cgggcacctc
     7921 aaagtggggg ttaagaatgt ccttacttta ccatgttcca ggaaaaacac cgagaaccct
     7981 tattgttgcc gtaatgttga ttttctgttt tgtaggtaag gtgttatgtt gccttgtcgt
     8041 accattatca acacgataat aattaataat tcatttttaa ataaattgct tcaagttaac
     8101 tcgctattta tttaactggg tttaaacgca tacctgtagt tcagaaatta tgagcgattt
     8161 ttgatagtga ccagatggca aaagaattat aaggaggaca cggtacgaca aaaaaggtag
     8221 atgaatcatg ttgaagataa tacccggtgc aactggatac tttaataaaa cactcaattc
     8281 aaatcaattc gataatgaag atgcaatcaa agataaatta gataatagag gttcaatcaa
     8341 aggtaaatta aataatatat atggtaaatc tattgattat gcagcgctgc gtcatcggga
     8401 tataattatt gcaaaaatag atttgtttat tcaacgaatc acgcataatt tatggcatgc
     8461 acgtaaaaaa atgtgttttt aattgagcaa ataaatgatc tcaaaatgtg ggtcaataag
     8521 tatattgacg attgcactga tgaggattta aacgatcgtg actttattgc atcagttgtt
     8581 gaccgggcta tttttcattt cgcgattaat agtatatgta atcctgggga taataaagat
     8641 gcgactccca ttgaacaatg tacttttgat gtagaaacta agaatggcct tccctccacg
     8701 gttcagctat tttatgagga atctaaggat aatgaacctt tagcgaatat acattttcaa
     8761 acaataggtt ctggtttttt aacgtttgtt aatgcctgcc aggaacatga tgacaacagc
     8821 ttaaaattat ttgcttcgct gttaatttca ctttcatatt ctagtgccta cgcagattta
     8881 tcagaaacag tgtatattaa tgaaaataat gagagctacc tgaaagctca gtttgaaaaa
     8941 ttatctcaac gtgatatgaa gaagtacctg ggagagatga agcgtctggc tgatggggga
     9001 gaaatgaatt ttgatggcta tctggataag atgtcacatc tggtgaatga agggacgctc
     9061 gatcctgata ttttaagcaa aatgcgagat gctgcaccac aattaattag cttcgcgaag
     9121 tcgtttgacc caacctcaaa ggaagagatt aaaatactta cagacacttc taaattaatt
     9181 tatgatttgt tcggggttaa atcggagaaa taatatgtga agttcttcga tagtatggaa
     9241 ggcattatat aaaaggaccc aatatttatt gggtcctttt ttctctatca atgctattag
     9301 cagggagata tatcaccaaa gtttaatgtg tgatttttta tttatcgtcg aacctggatt
     9361 gtttatcatt ggccttaaca aagttaacgg ctaataagat tatttccatc acttcgtgag
     9421 agcttcatgc cttgagagga tctcaatttt cttttgcaat gagacaggcg cttcctgttg
     9481 ttatggtata gtaccccgct attgagcctc ctgaatagtg atgctgaata acataacccc
     9541 atgatatatc gataaaataa tctctacatt tgaaaatgca cggtaattct gaaatgcaaa
     9601 aaatcaacca aaccagcgca atgcctgaaa aaactgacgt tcactggagt ggtcggttta
     9661 gcgttgcacc aatgctcgac tggacggaca gacattgccg ctatttcttg cgtctgcttt
     9721 cccgcaatac gttgctgtat accgaaatgg tgaccacagg ggcgattatt cacggtaaag
     9781 gtgattacct ggcgtacagt gaagaagaac atccggtagc gttgcaactc ggcggtagcg
     9841 atccggcggc gctggcacag tgtgcgaagc tggcagaagc gcgtggatat gatgagatca
     9901 acctgaatgt cggctgcccg tctgaccggg tgcagaacgg catgtttggt gcgtgtctga
     9961 tgggtaatgc gcagctggtt gccgattgcg tgaaagcgat gcgcgatgtg gtgtcgattc
    10021 cggtgacggt gaaaacgcgt attggcatcg atgaccagga cagctatgaa tttctctgcg
    10081 atttcatcaa caccgtttcc ggcaaaggcg agtgtgggat gttcatcatc catgcacgta
    10141 aagcctggct ttcggggtta agcccgaaag aaaaccgtga aatcccgccg ctcgattatc
    10201 cgcgtgtgta tcaactgaag cgtgactttc cgcatctgac aatgtcgatt aacggtggta
    10261 tcaagtcgct ggaagaggcc aaagcacacc tgcaacatat ggatggcgtg atggtcgggc
    10321 gcgaggcgta tcagaatccg ggtattctgg cggcggtaga ccgggagatc tttggttcct
    10381 cggataccga tgcctatccg gtggcggtag tgcgcgccat gtatccgtac attgagcgtg
    10441 aactcagcca ggggacgtat ctcggccata ttacccggca tatgttgggc ttgttccagg
    10501 gtattcctgg cgcgcggcag tggcggcgtt atttaagtga aaatgcccat aaagcgggtg
    10561 cagacattaa tgtgctggaa cacgcgctca aactggtggc ggataagcgt taacttttca
    10621 ccaaaaagta gtcaaattca ccacgccctg cgcaccgtcg cggggcgttt tgctgttaaa
    10681 tcaatagatt atttttggca tgattcttgt aatgccagca agagatttca tatttgggag
    10741 agcatcatgc tggaactact ttttgtgatt ggcttttttg tcatgctgat ggtcaccggc
    10801 gtttcgttgc tgggcattat cgccgcgctg gttgtggcga cggccattat gttcctcggc
    10861 ggtatgctgg cattgatgat taagttgctg ccgtggttac tactggcgat tgcggtggtg
    10921 tgggttatta aggcgattaa agcaccaaaa gtgcctaaat atcagcgtta tgaccgctgg
    10981 cgttactaag ggattgtgcg gatgatcaca acctaaggtt ttatccttag aacaaaatag
    11041 gaattgataa tcaaatctgt cactattgcg cctctaacag attcatcgtg ctgtacccta
    11101 catacagccg actataaaaa gaaagggctt cccaggtgga agccctattt cttttatgga
    11161 atcagcaggc tggaaccttg cgtcgcccgg ctttccagaa tctcatgcgc acgctgcgca
    11221 tccttcagcg gatatttctg ctgctcggcg acatcgacct taatcacacc gctggcaatc
    11281 aaagagaaca gttcattact ggcctcggtt aattcctccc gtgtggtgat atagccttgc
    11341 agggaagggc gtgtcacata caacgagcct ttttgattga gaatgcctaa gttcacaccg
    11401 gtaaccgcac ctgatgagtt gccaaaactg accattaagc cgcggcgttg caggcaatcc
    11461 agcgaccgtt cccaggtgtc tctgcccacg gaatcgtaca ctacgcgcac tttcttaccg
    11521 ccggtgatct ctttcaatcg ctcgaccaga ttctcttcac gatagttaat aacctgccac
    11581 gcgccggctt ttagcgcgct ctgcgctttt tgcgcggttc ctacggtgcc gataagtttc
    11641 gcgcccagag cttttgccca ctggcaggca atcaagccaa cgccgccagc cgctgcgtgg
    11701 aacaggaact gttcatcggg tttaatttca taggttttgc gcagcagata ataaaccgtt
    11761 aagcctttca ggaaagatgc tgcagcttgc tcaaaagaaa tagccgcagg cagaatcgcc
    11821 gctttatccg cattaatgtt atgcacagag ctgtaagcgc ctaacgccga ctgcgcatag
    11881 actacacgat cgcctgcctt aatatgcttt acaccactgc cgactttact cacgatgcct
    11941 gccgcttcgg tacctgatcc gctgggtagt gatggcggcg ggtaaaggcc gctgcggata
    12001 tacgtgtcga taaaattgat gccgatggct ttattttcga cctggatttc attctctgcc
    12061 ggatcggcag gagtgaactc tacggcttga agtacttccg ggccaccgtg cttgtgaaat
    12121 tcaattcgtg ttgccatgtg tcctccagaa cgtatcgtca gggtctgctt catatggtaa
    12181 agtttcgacc cattctttat ctcggtaact ccattcacta tggcaggaaa taaacccttc
    12241 aacaaacagc aggctgaacc ccgcgaacgc gatccacaag ttgccgggct gaaagtgcct
    12301 ccgcactcga tcgaagcgga gcagtcggtg ttgggcggtt taatgctgga taacgaacgc
    12361 tgggacgatg tagccgagcg tgtggtggca gacgattttt acacccgccc acaccgccat
    12421 atctttactg aaatggcgcg tttgcaggaa agcggtagtc ctatcgatct gattaccctt
    12481 gcggaatcgc tggaacgcca ggggcaactc gatagcgtcg gtggttttgc ttatctggca
    12541 gagctgtcaa aaaatacgcc aagtgcggcg aacatcagtg cttatgctga catcgtgcgt
    12601 gaacgtgccg ttgtccgtga gatgatctcg gttgcgaatg agattgctga agccggtttt
    12661 gatccgcagg ggcgtaccag cgaagatctg ctggaccttg ctgaatcgcg cgtctttaaa
    12721 attgccgaaa gtcgtgcaaa caaagacgaa gggccgaaga acatcgccga tgtgctcgac
    12781 gcaaccgtgg cgcgtattga gcagttgttt cagcagccac acgatggcgt taccggagta
    12841 aacaccggtt atgacgatct caacaaaaaa accgctggct tgcagccgtc ggatttgatc
    12901 atcgtcgccg cgcgtccgtc gatgggtaaa acaacatttg cgatgaacct cgtcgaaaac
    12961 gcggcgatgt tgcaggataa accagtactt atcttctcgc tggagatgcc ttcagaacag
    13021 attatgatgc gttctctggc gtcgctgtcg cgcgttgacc agactaaaat ccgtaccggg
    13081 cagctcgatg atgaagactg ggcgcgcatt tccggcacca tgggtatttt gctcgaaaaa
    13141 cgcaatatct atatcgatga ctcctccggc ctgacgccaa cggaagtgcg ttcccgcgca
    13201 cgccgtattg cccgtgaaca cggcggcatc gggcttatca tgatcgacta cctgcaactg
    13261 atgcgcgtac cggcgctttc cgataaccgt acgctggaaa ttgcagaaat ctctcgctcg
    13321 ctgaaagcac tggcgaaaga actgaacgtg ccggtggtgg cgctgtccca gttgaaccgt
    13381 tctctggaac aacgtgccga caaacgcccg gtcaactccg acctgcgtga atctggctct
    13441 atcgagcagg atgcggactt gatcatgttt atctatcgtg atgaggtgta tcacgaaaac
    13501 agtgatttaa aaggcatcgc ggaaattatt atcggtaaac aacgtaacgg cccaatcggg
    13561 acggtacgcc tgacctttaa cggtcaatgg tcgcgcttcg acaactatgc ggggccgcag
    13621 tacgacgacg aataataatt attttatgaa ttaggcaatt aaagcaaaca cttatcaagg
    13681 aacacaaatg caagcggcaa ctgttgtgat taaccgccgc gctctgcgac acaacctgca
    13741 acgtcttcgt gaactggccc ctgccagtaa aatggttgcg gtggtgaaag cgaacgctta
    13801 tggtcacggt cttcttgaga ccgcgcgaac gctccccgat gctgacgcct ttggcgtagc
    13861 ccgtctcgaa gaagctctgc gactgcgtgc ggggggaatc accaaacctg tactgttact
    13921 cgaaggcttt tttgatgcca gagatctgcc gacgatttct gcgcaacatt ttcataccgc
    13981 cgtgcataac gaagaacagc tggctgcgct ggaagaggct agcctggacg agccggttac
    14041 cgtctggatg aaactcgata ccggtatgca ccgtctgggc gtaaggccgg aacaggctgg
    14101 ggcgttttat catcgcctga cacagtgcaa aaacgttcgt cagccggtga atatcgtcag
    14161 ccattttgcg cgcgcggatg aaccaaaatg tggcgcaacc gagaaacaac tcgctatctt
    14221 taataccttt tgcgaaggca aacctggtca acgttccatt gccgcgtcgg gtggcattct
    14281 gctgtggcca caatcgcatt ttgactgggt gcgcccgggc atcattcttt atggcgtctc
    14341 gccgctggaa gatcgctcca ccggtgccga ttttggctgt cagccagtga tgtcactaac
    14401 ctccagcctg attgccgtgc gtgagcataa agccggagag cctgttggtt atggtggaac
    14461 ctgggtaagc gaacgtgata cccgtcttgg cgtagtcgcg atgggctatg gcgatggtta
    14521 tccgcgcgcc gctccatccg gtacgccagt gctggtgaac ggtcgcgaag taccgattgt
    14581 cgggcgcgtg gcgatggata tgatctgcgt agacttaggt ccacaggcgc aggacaaagc
    14641 cggggatccg gtcattttat ggggcgaagg tttgcccgta gaacgtatcg ctgaaatgac
    14701 aaaagtgagc gcttacgaac ttattacgcg cctgacttca agggtcgcga tgaaatacgt
    14761 ggattaatcg ttctgtaata tttgattgtc tgtgccggat gcggcgtgaa tgccttatcc
    14821 ggccaataaa atcctaaaaa ttcaataagt tgatgttctt tcatgctctt ataaaggtcg
    14881 tgcctctggc ggatgttcgt ttgtcatgag tctcactctg ttgctaattg ccgttcgctc
    14941 ctgaacatcc actcgatctt cgccttcttc cggtttattg tgttttaacc acctgcccgt
    15001 aaacctggag aaccatcgcg tgtttcaaaa agttgacgcc tacgctggcg acccgattct
    15061 tacgcttatg gagcgtttta aagaagaccc tcgcagcgac aaagtgaatt taagtatcgg
    15121 tctgtactac aacgaagacg gaattattcc acaactgaaa gccgtggcgg aggcggaagc
    15181 gcgcctgaat gcgcagcccc atggcgcttc gctttattta ccgatggaag ggcttaacag
    15241 ctatcgccat gccattgcgc cgctgctgtt tggtgcggac catccggtac tgcaacaaca
    15301 gcgcgtagca accattcaaa cccttggcgg ctcaggggca ttgaaagtgg gcgcggattt
    15361 cctgaaacgc tacttcccgg aatcaggcgt ctgggtcagc gatcctacct gggaaaacca
    15421 cgtagcaata ttcgccgggg ctggattcga agtgagtact tacccctggt atgacgaagc
    15481 gactaacagc gtgcgcttta atgacctgtt ggcgacgctg aaaacattac ctgcccgcag
    15541 tattgtgttg ctgcatccat gttgccacaa cccaacaggt gccgatctca ctaatgatca
    15601 gtgggatgcg gtgattgaaa ttctcaaagc ccgcgagctt atcccattcc tcgatattgc
    15661 ctatcaagga tttagtgccg gtatggaaga ggatgcctac gctattcgcg ccattgccag
    15721 cgctggatta cccgctctgg tgagcaattc gttctcgaaa attttctccc tttacggcga
    15781 gcgcgtcggc ggactttctg ttctgtgtga agatgccgaa gccgctggcc gcgtactggg
    15841 gcaattgaaa gcaacagttc gccgcaacta ctccagcccg ccgaatttgg gtgcgcaggt
    15901 ggtggctgca gtgctgaatg acgaggcatt gaaagccagc tggctggcgg aagtagaaga
    15961 gatgcgtact cgcattctgg caatgcgtca ggaattggtg aaggtattaa gcacagagat
    16021 gccagaacgc aatttcgatt atctgcttaa tcagcgcggc atgttcagtt ataccggttt
    16081 aagtgccgct caggttgacc gactacgtga agaatttggt gtctatctca tcgccagcgg
    16141 tcgcatgtgt gtcgccgggt taaatacggc aaatgtacaa cgtgtggcaa aggcgtttgc
    16201 tgcggtgatg taatgcagga aagcaggctg gagttaccca gcctgcagtg aaattaaact
    16261 gtcgtcgctt tcactatttc tttgtagatg atttttttga tgccgttgtt ctacgtgaga
    16321 gataataaaa cgttgtcagt tcttttattg ttaagtttat cccaattaac tggaattcct
    16381 t
//
LOCUS       DADLFA010000091        16378 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000091 DADLFA010000000
VERSION     DADLFA010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16378
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.091"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..309
                     /locus_tag="JQH65_002422"
     CDS             <1..309
                     /locus_tag="JQH65_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HAY6139809.1"
                     /translation="IIVQWDANSNGIWDREPVKESDQIGFRLKEHVLETLRGATSCEG
                     KGWDKVTNPDAIIIDTFQVVRQDVSGFSPVLTVNMRAASKSEPQTVVNASYSVTGFNL
                     "
     gene            306..713
                     /locus_tag="JQH65_002423"
     CDS             306..713
                     /locus_tag="JQH65_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HAY6139810.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAETDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGNIVFSPSGWSDFCPLKERALCQLP"
     gene            698..1021
                     /locus_tag="JQH65_002424"
     CDS             698..1021
                     /locus_tag="JQH65_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAY6139811.1"
                     /translation="MSASLKNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRLQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            1034..4402
                     /gene="recC"
                     /locus_tag="JQH65_002425"
     CDS             1034..4402
                     /gene="recC"
                     /locus_tag="JQH65_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HAY6139812.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEHEDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPEWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRLWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGLSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDNI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            4578..7466
                     /gene="ptrA"
                     /locus_tag="JQH65_002426"
     CDS             4578..7466
                     /gene="ptrA"
                     /locus_tag="JQH65_002426"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HAY6139813.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTQARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTTEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            7459..11001
                     /gene="recB"
                     /locus_tag="JQH65_002427"
     CDS             7459..11001
                     /gene="recB"
                     /locus_tag="JQH65_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HAY6139814.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIATVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPKSVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNEKGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIAMMDEMFAGMTLEEA"
     gene            11001..12827
                     /locus_tag="JQH65_002428"
     CDS             11001..12827
                     /locus_tag="JQH65_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RecBCD enzyme subunit RecD"
                     /protein_id="HAY6139815.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(12889..14220)
                     /gene="argA"
                     /locus_tag="JQH65_002429"
     CDS             complement(12889..14220)
                     /gene="argA"
                     /locus_tag="JQH65_002429"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HAY6139816.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            14452..15705
                     /locus_tag="JQH65_002430"
     CDS             14452..15705
                     /locus_tag="JQH65_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HAY6139817.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            15965..16117
                     /locus_tag="JQH65_002431"
     CDS             15965..16117
                     /locus_tag="JQH65_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAY6139818.1"
                     /translation="MIIEKVMNNNCVQASVNGQEVIISGPGVGYNKNMVCRSLSILQT
                     GFLCQK"
     gene            16105..>16378
                     /locus_tag="JQH65_002432"
     CDS             16105..>16378
                     /locus_tag="JQH65_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAY6139819.1"
                     /translation="MSEMSKKNKLYKLIEHVGIEYIFVAEKIVHYAESNLEKKLNPSL
                     LLILADHISNAISRVVSGIQINNVFLEEIKALYKAEYAISRDALTII"
BASE COUNT         3914 a         4034 c         4532 g         3898 t
ORIGIN      
        1 atcattgtgc agtgggatgc gaacagtaac ggtatctggg atcgcgaacc ggtaaaagag
       61 tccgaccaga ttggatttcg tctgaaggag catgtgctgg aaacgctacg cggtgcgaca
      121 tcctgtgaag gtaagggctg ggataaagtc actaatccgg atgccatcat tatcgacact
      181 tttcaggtcg tacgtcagga tgtcagcggc ttctcgccgg tgttgacggt taatatgcgt
      241 gctgccagta agtctgaacc gcaaaccgtg gtgaatgcca gctatagcgt gacaggattc
      301 aacctgtgaa ccgcgaaaag ggtgtttcgt cactggctct ggtcctgatg ctgctggttt
      361 tgggtagttt gctattacaa ggaatgagcc aacaggatcg cagttttgcc tctcgcgtga
      421 gcatggaaag tcagtcattg cgccgccagg ccatcgttca gtcggcgctg gcgtggggaa
      481 aaatgcactc ctggcagacg cagccagcag ttcagtgctc gcagtacgct gaaaccgatg
      541 cccaggtttg tttgcgttta ctggcagata atgaagcctt attgattgcc ggttatgaag
      601 gcgtttcgtt gtggcgaaca ggcgaagtca ttgatggaaa cattgttttt tcgccaagcg
      661 gctggagcga tttttgtccg ctgaaagaga gggcgttatg tcagcttccc tgaagaatca
      721 acaaggcttt agcctgccgg aggtaatgtt ggcgatggtg ttgatggtga tgattgtcac
      781 tgcgttatcg ggtttccagc gaacattaat gaacagtctt gccagcagaa accagtacca
      841 acagctctgg cggcatggct ggcagcaaac gcaactgcgc gcgatttcgc caccagctaa
      901 ctggcaggtc aaccgattgc agacatcgca ggcgggatgt gtcagcatca gcgttacgct
      961 agtttcaccc gggggcagag aaggcgagat gacccgcctg cattgcccga atcgtcagta
     1021 gtcaggagcc gctatgttaa gggtctacca ttccaatcgt ctggacgtgc tggaagcgtt
     1081 gatggagttt attgtcgaac gcgaacggct ggacgatcct ttcgaaccag agatgattct
     1141 ggtgcaaagt accggtatgg cacagtggct gcaaatgacc ctgtcgcaaa agtttggtat
     1201 tgcggcaaac attgattttc cgctgcctgc gagctttatc tgggatatgt tcgtccgggt
     1261 gttaccggag atccccaaag agagcgcctt taacaaacag agcatgagct ggaaactgat
     1321 gactctgctg ccgcaactgt tggagcacga agactttacc ctgttgcggc attatctgac
     1381 tgacgatagc gacaagcgaa aactgttcca gctttcctca aaagcggcgg acctgtttga
     1441 ccagtatctg gtctatcgcc cggagtggct ggcacaatgg gaaacaggac atttggttga
     1501 agggctggga gaagcacagg cctggcaagc gccgttgtgg aaggcgctgg tggaatatac
     1561 ccatgaactc ggacaaccgc gctggcaccg cgcgaatctc tatcagcgct ttatcgaaac
     1621 gctggagtcc gcgacgacct gcccgccggg gttaccttcg cgcgtcttta tatgcggtat
     1681 ttccgcgtta ccgcctgttt atctccaggc gctccaggcg ctgggtaaac atattgaaat
     1741 ccatctcctg tttaccaacc cctgccgtta ttactggggc gatattaaag atcctgctta
     1801 tctggcgaaa ctgctgactc gtcagcgtcg acacagtttt gaagatcgcg aattgccgct
     1861 atttcgcgat agcgaaaatg ccggacagct ctttaacagc gatggtgaac aggatgtcgg
     1921 caacccgctg ctggcctcat gggggaagct tgggcgcgac tacatttatc tcctttctga
     1981 cctggagagc agccaggagc tggacgcctt tgtcgatgtc acgccagata acctgctgca
     2041 taacattcag tctgacattc tggaactgga aaaccgtgcc gttgctggtg tgaacatcga
     2101 agagttttcc cgtagcgata acaaacgccc gcttgatcca ctggatagca gtatcacctt
     2161 ccacgtttgc catagcccgc agcgtgaagt tgaagttttg cacgatcgcc tgctggcgat
     2221 gctggaagaa gacccgacac ttactccgcg cgacatcatc gtgatggtgg ctgatatcga
     2281 cagctacagt ccgtttattc aggctgtgtt tggtagcgca cctgcggatc gttacctgcc
     2341 ttacgccatt tccgaccgtc gggcgcggca gtcgcatccg gtactggaag cgtttatcag
     2401 cctgttatcg ttgccagaca gccgctttgt gtcggaagac gtgctggcat tactggatgt
     2461 gccggtgctg gcggcgcggt ttgacatcac cgaagaaggg ctgcgttatt tacgcctgtg
     2521 ggtcaacgaa tccggcattc gttgggggat agatgacgac aacgttcgcg agctggaact
     2581 ccccgccacc ggacaacaca cctggcgatt tggcctgacg cgtatgttgc tgggctacgc
     2641 gatggagagc gcgcagggcg agtggcaatc ggttctacct tatgatgagt cgagcggctt
     2701 aattgcagaa ctggtggggc atctggcttc actgctaatg cagctaaaca tctggcgtcg
     2761 cgggctggca caggagcgtc cgctagaaga gtggttgccg gtttgtcgcg atatgctcaa
     2821 cgccttcttc ctgccggatg cggaaaccga agcggcgatg acgctgatcg aacaacaatg
     2881 gcaggcgatt atcgccgaag gtttaggtgc gcagtatggc gacgcggtgc cgctgtcact
     2941 attgcgtgat gaactggcac agcgtctgga tcaagaacgt atcagccagc gttttctcgc
     3001 cggaccggtt aacatttgta ctctgatgcc aatgcgttca attccgttca aagtggtttg
     3061 cctgctggga atgaacgacg gcgtttatcc acgtcagctt gcgccattgg gctttgacct
     3121 gatgagccag aaaccgaagc gtggcgaccg tagccgtcgc gatgacgacc gctacctgtt
     3181 cctggaagcg ttaatttccg cgcagcaaaa actctatatc agttatatcg ggctttccat
     3241 tcaggataac agtgaacgtt tcccgtcggt actggtgcag gaactgatcg actacatcgg
     3301 gcaaagtcat tatctaccgg gcgatgaagc gctcaactgt gatgaaagcg aggcaagggt
     3361 aaaagcgcat cttacctgcc tccatacccg gatgccgttt gatccacaaa actaccagcc
     3421 aggcgaacga caaagctatg ctcgtgaatg gctacctgcg gccagccagg ctggtaaagc
     3481 acattctgaa tttgttcagc cgctgccgtt taccttaccg gaaaccgtgc cgctggaaac
     3541 gctacaacga ttctgggcac atccggtgcg ggcatttttc cagatgcgtt tgcaggtgaa
     3601 cttccgtacc gaagacagcg aaatccccga caccgagcca tttattctgg aagggcttag
     3661 ccgttatcaa atcaatcagc agttattgaa tgcactggtt gagcaggatg atgccgaacg
     3721 cttgttccgt cgcttccggg cggcagggga tttaccgtat ggcgcttttg gtgaaatttt
     3781 ctgggaaact cagtgccagg agatgcagca gcttgccgac agagtcattg cctgtcgcca
     3841 gccggggcag agtatggaaa ttgatctcgc ctgcaacggt gtgcagataa ctggctggtt
     3901 gccgcaggtg cagccggatg gcctgttgcg ctggcgtccc tctttattaa gtgtggcgca
     3961 gggaatgcaa ctttggctgg aacaccttgt ctactgtgcc agcggtggta atggtgaaag
     4021 tcgccttttt ctacgcaaag acggcgagtg gcgttttccg ccgcttgcag ccgaacaggc
     4081 tttgcattac ctctcacaac tgattgaggg gtatcgtgaa gggatgtccg cgccattgct
     4141 ggtgttacct gaaagtggcg gcgcgtggct aaaaacctgt tatgacgcgc aaaacgatgc
     4201 catgctggat gacgattcca cgttgcaaaa agcccgtacg aaattccttc aggcttacga
     4261 aggcaacatg atggtgcgtg gcgaaggtga taatatctgg tatcaaaggc tctggcggca
     4321 attaacacca gagacaatgg aggccatcgt tgaacagtcg caacgtttcc tgttaccgct
     4381 gtttcgcttt aatcagtcat gagtgctgta taaaaattgc gcaatctatc cgcttacttt
     4441 atgatgcgca ccagtcacgg actgatggtt atataaacat aggctgactc gtgcagcaca
     4501 agattaaatt ctggcagatg atttgcgtta acgtgttgaa tctggacaga aaattaagtt
     4561 gattatgagg tccgtgaatg ccccgcagca cttggttcaa agcattattg ttgttagttg
     4621 ccctttgggc acccttaagt caggcagaaa cgggatggca gccgattcag gaaaccatcc
     4681 gtaaaagtga taaagataac cgccagtatc aggctatacg tctggataac ggtatggtgg
     4741 tcttgctggt ttctgatccg caggcagtta aatcgctctc ggcgctggtg gtgcccgttg
     4801 ggtcgctgga agatcccgag gcgtaccagg ggctggcaca ttaccttgaa catatgagtc
     4861 tgatggggtc gaaaaagtac ccgcaggctg acagtctggc cgaatatctc aaaatgcacg
     4921 gcggtagtca caatgccagc actgcgccgt atcgcacggc tttctatctg gaagttgaga
     4981 acgacgcctt gcctggtgcg gtagaccgcc tggccgatgc tattgctgaa cctttgctcg
     5041 acaagaaata tgccgaacgt gagcgtaatg cggtgaacgc tgaattaacc atggcgcgta
     5101 cgcgtgacgg gatgcgcatg gcacaggtca gcgcagaaac cattaacccg gcacaccccg
     5161 gttcaaagtt ttctggtggt aacctcgaaa ctttaagcga taaacctggt aatccggtgc
     5221 agcaggcgct gaaagatttc cacgagaagt actattccgc caatctgatg aaggcggtta
     5281 tttacagcaa taaaccgctg ccggagttgg caaaaatggc ggcggacacc tttggtcgcg
     5341 tgccgaacaa agagagcaaa aaaccggaaa tcaccgtgcc ggtagtcacc gacgcgcaaa
     5401 agggcattat cattcattac gtcccggcac tgccgcgtaa agttctgcgc gttgagtttc
     5461 gcatcgataa caactcagcg aagttccgta gtaaaacgga tgaattgatt acctatctga
     5521 ttggtaatcg cagcccaggt acactttccg actggctgca aaagcaggga ttagttgagg
     5581 gcattagcgc caactccgat cctatcgtca acggcaacag cggcgtatta gcgatctctg
     5641 cgtctttaac cgataaaggc ctggctaatc gcgatcaggt tgtggcggca atttttagct
     5701 atctcaatct gttacgtgaa aaaggcattg ataaacaata cttcgatgaa ctggcgaatg
     5761 tgctggatat cgacttccgt tatccgtcga tcacccgtga tatggattac gtcgaatggc
     5821 tggcagatac catgattcgc gttccggttg agcatacgct ggatgcagtc aatattgccg
     5881 atcggtacga tgctaaagca gtaaaagaac gtctggcgat gatgacgccg cagaatgcgc
     5941 gtatctggta tatcagcccg aaagagccgc acaataaaac ggcttatttt gtcgatgcgc
     6001 cgtatcaggt cgataaaatc agcgaacaaa ctttcgctga ctggcagcaa aaagctgcca
     6061 atattgcgct ctccttaccg gagcttaacc cctatattcc tgacgatttc tcgctgatta
     6121 agtcagagaa gaaatatgac catccagagc tgattgttga tgagtcgaat ctgcgcgtgg
     6181 tgtatgcgcc aagccgttat ttttccagcg aacccaaagc tgatgtcagc ctgattttgc
     6241 gtaatccgaa agccatggac agcgcccgca atcaggtgat gtttgcgctc aatgattatc
     6301 tcgcagggct ggcgcttgat cagttaagca accaggcgtc ggttggtggc ataagttttt
     6361 ccaccaacgc taacaacggc cttatggtta atgctaatgg ttacacccag cgtctgccgc
     6421 agctgttcca ggcattgctc gaggggtact ttagctatac cgctacggaa gatcagcttg
     6481 agcaggcgaa gtcttggtat aaccagatga tggattccgc agaaaagggt aaagcgtttg
     6541 agcaggcgat tatgcccgcg cagatgctct cgcaagtgcc gtacttctcg cgagatgaac
     6601 ggcgtaaaat tttgccctcc attacgttga aagaggtgct ggcctatcgc gacgccttaa
     6661 aatcaggggc tcgaccagag tttatggtta tcggcaacat gaccgaggcc caggcaacaa
     6721 cgcaggcacg cgatgtgcaa aaacagttgg gcgctgatgg ttcagagtgg tgtcgaaaca
     6781 aagatgtagt ggtcgataaa aaacaatccg tcatctttga aaaagccggt aacagcaccg
     6841 actccgcact ggcagcggta tttgtaccga ctggctacga tgaatacacc agctcagcct
     6901 atagctctct gttggggcag atcgtacagc cgtggttcta caatcagttg cgtaccgaag
     6961 aacaattggg ctatgccgtg tttgcgtttc caatgagcgt ggggcgtcag tggggcatgg
     7021 gcttcctttt gcaaagcaat gataaacagc cttcattctt gtgggagcgt tacaaggcgt
     7081 ttttcccaac cacagaggca aaattgcgag cgatgaagcc agatgagttt gcgcaaatcc
     7141 agcaggcggt aattacccag atgctgcagg caccgcaaac gctcggcgaa gaagcatcga
     7201 agttaagtaa agatttcgat cgcggcaata tgcgcttcga ttcgcgtgat aaaatcgtgg
     7261 cccagataaa actgctgacg ccgcaaaaac ttgctgattt cttccatcag gcggtggtcg
     7321 agccgcaagg catggctatt ctgtcgcaga tttccggcag ccagaacggg aaagccgaat
     7381 atgtacaccc tgaaggctgg aaagtgtggg agaacgtcag cgcgttgcag caaacaatgc
     7441 ccctgatgag tgaaaagaat gagtgatgtc gccgagacac tagatccttt gcgcttgccc
     7501 ttacagggtg agcgcctgat tgaagcctct gccggcacag gcaaaacctt tacgattgcg
     7561 gcgctctatt tgcgcctgtt acttggacta ggcggttccg ccgcctttcc ccgcccgctg
     7621 accgttgaag aactgctggt ggtcaccttt accgaggctg ccacggcaga attgcgtggt
     7681 cgtatccgta gcaatatcca tgagttgcgc atcgcctgtc tgcgtgaaac taccgacaat
     7741 ccactgtacg aacgtctgct ggaagagatc gacgataaag cgcaagccgc gcagtggttg
     7801 ttgttagccg aacggcagat ggatgaagcg gcagtcttta ctattcacgg tttttgccag
     7861 cgcatgctca acctgaatgc ctttgaatcc ggcatgctgt ttgagcagca gctgattgaa
     7921 gatgagtctc tgctacgtta ccaggcctgc gccgatttct ggcgtcgcca ctgctacccg
     7981 ctgccgcgtg aaatagccca ggtcgtcttt gaaacctgga aagggccgca ggcgttgctg
     8041 cgcgatatta atcgttatct gcaaggcgaa gcgccggtta tcaaagcacc gccgcccgat
     8101 gatgaaacgc tggcttcccg ccacgcgcaa attgtggcgc gtattgctac ggtaaaacag
     8161 cagtggcgcg acgcagtggg tgaactggat gcgctgatcg aatcttctgg tattgatcga
     8221 cgcaagttta accgtagcaa tcaggctaaa tggatcgaca agatcagcgc ctgggcagaa
     8281 gaagagacca acagttatca gttgccggag tcgctggaaa aattctccca gcgtttctta
     8341 gaagatcgca cgaaagctgg gggggaaacc ccgcgacatc cactgtttga ggcgatcgat
     8401 caactgcttg cagaaccatt gtcgatccgc gatctggtga tcacccgcgc attggctgag
     8461 atccgcgaaa cagtagcgcg tgaaaaacgc cgccgtggcg aattgggttt tgatgacatg
     8521 ttaagtcggc tcgattccgc gctgcgtagc gaaagcggtg aggtgttggc agcggcgatc
     8581 cgtacgcgat tcccggtggc aatgatcgat gaatttcagg ataccgaccc ccagcagtac
     8641 cgaatttttc gccgtatctg gcaccatcag ccggaaaccg cattgttgct gattggcgac
     8701 ccgaagcagg ccatatatgc attccggggt gcggatatct tcacttatat gaaggcgcgt
     8761 agtgaagttc acgcccacta cactttagac accaactggc gctccgcacc tggaatggtg
     8821 aacagtgtga acaagctttt cagccagact gatgacgcgt tcatgtttcg cgaaataccg
     8881 tttattccag tgaaatcagc cgggaaaaat caggcgttac gttttgtatt taaaggtgaa
     8941 acgcagcctg cgatgaaaat gtggctgatg gaaggcgaaa gctgcggcgt tggcgactat
     9001 caaagtacca tggcgcaggt atgtgctgcg caaatccgcg actggctaca agccggacag
     9061 cggggcgaag cgttgctgat gaacggcgac gacgcgcgtc cggtgcgtgc ttcggacatc
     9121 agtgtgctgg tgcgcagccg ccaggaggcc gcccaggtgc gcgatgcctt aacgttgctg
     9181 gaaatccctt ccgtttacct ttcgaaccgc gacagtgttt ttgaaactct ggaagcgcag
     9241 gaaatgcttt ggttattgca ggcggtgatg acgcccgaac gtgagaacac cctgcgtagt
     9301 gcgctggcaa cgtcaatgat ggggctgaac gcgctggata tcgaaacgct gaataatgac
     9361 gaacatgcgt gggatgcggt agttgaagag ttcgatggtt atcggcaaat ctggcgcaaa
     9421 cgtggcgtta tgccgatgct gcgggcgctg atgtcggcgc gtaacattgc agaaaacttg
     9481 ctggcaacgg caggcggtga gcggcgtctt accgatatct tgcatatcag cgaactgcta
     9541 caagaagccg gaacgcagct ggaaagtgaa catgcgctgg tacgctggtt atcgcaacat
     9601 atcctcgagc cagacagtaa tgcctccagc caacaaatgc gcctcgaaag tgataaacat
     9661 ctggtgcaga ttgtcacgat ccataaatcg aaagggctgg aatatccgct ggtctggttg
     9721 ccatttatta ccaatttccg cgtccaggat caggcgtttt atcacgatcg ccactcgttt
     9781 gaggcagttt tggatcttaa tgctgcgcca aaaagcgtcg acctcgcgga ggtcgaacgt
     9841 ctggcggaag atctgcgttt gctttacgtg gcgctgacgc gttcggtttg gcattgcagt
     9901 ctcggcgttg caccgctggt gcgccgtcgt ggcgataaaa aaggtgacac cgacgtccac
     9961 caaagtgcgc tcgggcgttt gctgcaaaaa ggggaaccgc aagatgcggc agggcttcgc
    10021 acctgtattg aagcgttatg cgatgatgat attgcctggc aaacggcaca aactggtgat
    10081 aaccagccct ggcaggttaa tgatgcttta actgcagaac tgaatgcgag gacactacaa
    10141 cgattgcccg gcgataactg gcgtgtcacc agctactccg gcttgcagca gcgtggtcac
    10201 ggtatcgctc aggatctgat gccacggctg gatgttgatg ccgcaggcgt ggtcagcgtc
    10261 gttgaagaac cgacgttaac accgcatcag ttcccgcgcg gtgcgtcacc ggggacattc
    10321 ttgcacagtt tgtttgaaga cctcgatttt acccagccgg ttgacccgaa ctgggtacag
    10381 gaaaaactgg aactcggcgg ctttgaatcg cagtgggaac cggtgttgac cgagtggatc
    10441 acggctgtcc tccaggcacc tctcaatgaa aagggggtta gcctgagtca gctttccgac
    10501 cgcgataaac aggtggagat ggagttttac ctgccgatta gcgaaccgct catcgccagc
    10561 cagcttgatg cactaattcg ccagtttgat ccgctatccg ctggctgccc gccgctggag
    10621 ttcatgcagg tacgcggcat gttaaaaggc tttatcgacc tagtattccg ccacgaaggg
    10681 cgttattacc tgctcgacta taaatccaac tggctgggtg aagacagttc ggcttacacc
    10741 caacaggcta tggcagcagc gatgcaggca caccgctatg atctgcaata tcagctttat
    10801 accctggcgc tgcatcgtta tctgcgccat cgcattgctg attacgacta tgatcgtcac
    10861 tttggcggcg ttatctatct gttcctgcgt ggcgttgata aagaacatcc gcaacaaggg
    10921 atttacacaa cccgacccaa cgccgggttg attgccatga tggatgagat gtttgccggt
    10981 atgaccctgg aggaggcgta atgaaattgc aaaagcaatt actggaagct gtggagcaca
    11041 aacagctacg cccgctggat gtgcaatttg ccctgaccgt ggcgggagat gaacatcctg
    11101 ccgtcaccct cgcggcggca ctgttaagtc atgatgccgg agagggacac gtctgtttgc
    11161 cgctttcacg actggaaaat aacgaggcgt cgcatccgct gttggcgacc tgtgtcagtg
    11221 aaatcggtga gctacaaaat tgggaagaat gcttgctggc ttctcaagcg gtcagcaggg
    11281 gagatgaacc cacgccgatg atcctctgtg gcgatcgtct ttatttgaat cgcatgtggt
    11341 gtaacgagcg gacggtagcg cgcttcttca atgaggtgaa tcatgccatt gaggttgatg
    11401 aagctctact ggcgcaaacc ctggacaaac tttttccagt aagcgatgaa attaactggc
    11461 aaaaagttgc agcggcagtg gcgctgacgc ggcggatctc ggtgatttcc ggcggcccag
    11521 gcaccggtaa aacgaccacc gtggcgaagt tgctggcagc gttaattcaa atggccgacg
    11581 gcgaacgctg ccgtatccgt ctggctgcac caacgggtaa agctgccgcg cgcttaaccg
    11641 aatctctcgg caaggctttg cgacagttac cgctgaccga tgaacaaaag aaacgcattc
    11701 cggaagatgc cagcactttg caccgattgc tgggcgcgca gccgggtagc cagcgtttac
    11761 gtcatcatgc cggtaacccg ctgcatcttg atgtgctggt ggtagatgaa gcgtcaatga
    11821 tcgatctgcc tatgatgtcg agactgatcg acgccttgcc cgatcatgcg cgagtgatct
    11881 ttctcggcga tcgtgatcaa ctggcctcgg ttgaggctgg ggctgtgctg ggcgatatct
    11941 gcgcttatgc caacgcgggc tttaccgccg agcgtgccag gcagctaagc cgcctgacgg
    12001 ggactcacgt tccggcagga accggcacag aagcggcatc tttgcgcgac agtctctgcc
    12061 tgctgcaaaa aagctatcgt ttcggcagcg attctggcat tggtcagtta gctgcggcga
    12121 tcaaccgtgg tgataaaacg gcagtgaaaa ccgtttttca gcaggatttt actgatatcg
    12181 aaaaacgact tttacagagc ggcgaagatt atattgcgat gcttgaggaa gctcttgcgg
    12241 gttatggacg ttatctggat ctgctgcaag cgcgtgccga gccggattta atcattcagg
    12301 cgttcaatga gtaccaactt ttgtgcgccc tgcgggaagg gccgtttggc gtggctggac
    12361 tgaatgagcg aattgagcag tttatgcaac agaagcgcaa aattcatcgt catccgcact
    12421 ctcgttggta cgaaggtcga ccggtgatga ttgcccgtaa tgacagcgcg cttgggttgt
    12481 ttaatggcga tatcggtatt gcgctggatc gcgggcaggg gacgcgcgtc tggtttgcga
    12541 tgccggacgg caatattaag tctgtgcaac cgagtcgctt gccagagcac gaaactacgt
    12601 gggcgatgac ggtacataaa tcgcagggat cggagttcga ccatgcggcg ttgattttac
    12661 cgagccaacg cacgccggta gtaacgcgag agctggtata caccgcggtg acccgcgcgc
    12721 gtcgccgtct gtcgctgtat gccgatgagc gtatattaag tgcggcaatc gccactcgta
    12781 ctgagcggcg cagtggtctg gcggcgttgt ttagttcgcg ggaataaacg taattgccgg
    12841 atgcgatgca cgagtgttac gcatgtcgca tccgacaatt ttcatcgctt accctaaatc
    12901 cgccatcaac actttggatt tacgctggta gttgtacaac tgctttttgc tctcgggcag
    12961 taaatcaata tccaccgggg taaatccacg ttcctggaac cagtgaatac tgcgcgtggt
    13021 cagcacaaac aatttgctta agccgctctg cttcgcctga gcggcaatgc gttccagcag
    13081 aacctcgccc cgtgatgaac tgcggtaatc cgggtgaact gccacacagg ccatttcccc
    13141 aatcttctct tccgggaacg gatagagcgc ggcgcaggca atagtcgtgt tatcgcgctg
    13201 aataatggtg aatttgtcga tttccatctc cagctgctcg cgagaacggc gcaccagaat
    13261 accttgctgc tccagcgggc gaatcaactc cagaataccg ccaatatcgt tgattgttgc
    13321 gcgacgaatc tgctcggcgc tttccatcac aatctgcgta ccgataccgt cgcgtgagaa
    13381 caactcttgc aacagcgcgc catcttcctg ataactgatt aaatgacagc gacgcacgcc
    13441 gctgcggcag gctttcactg cgccacgcaa aaagcgcacc gtaccggagt tgtaatcgcc
    13501 tttctcttcc tgggcttcta cccgcgcttg cgcttcgtta gggaaaagtt ctgagacaat
    13561 atcaccgtcg tcattagtga cgccctgtga agagcaaaaa ccaatcatct tctcagcttt
    13621 cagtttgatg gccagttgag tggcaatctc ttccgaggtc agattaaagc tctcgccagt
    13681 gactgaaaca gcgaccggcc ccattagcac tattgcaccg ctgtccagtt gacgatggat
    13741 cgcgtcttca tcaatccgcc ggatacgccc gctatggcag taatccacgc cgtcatcgac
    13801 gcccagcggc tgggcaataa taaaattgcc actgacgacg ttgatatgcg cgccctgtag
    13861 cggcgtgtta ttgagactca tcgacaggcg agcagtgatg tccagttgca atgttcccgc
    13921 agcctgcttc accagttcca gtgttttggc gtcggtcaca cgtatattct tgtgatacag
    13981 cggttcgtgg tgatgcgcgg ccagatttgc gtcgatctgc ggacgtgcgc catagaccac
    14041 caccagacgg atgccgaggc tgtgcaacaa cccgatatca ttaacgatac tggagaaatt
    14101 ctcatgctca atggcttcac cgccgagcat gatgacaaac gtttttcccc ggtgggtatt
    14161 gatataggga accgaatggc ggaatccctc gaccagctcg gttttacgtt cctttaccac
    14221 ggcacacccc tttgcatgat tattctaaat tagtgtattt ttattctgtt ttttcgcagg
    14281 gtgcaagtgt aaattttatg cgagagcgac ttttttatca gtaatgccgt gaatataaaa
    14341 agaatgttta ccgtttatta gatgacagat tatgcgtctt tcgctaaagt ttccggtcaa
    14401 attagtcgtt tacttgttac acagcttaga tttcttttgc tcgggagagg catgtcagga
    14461 tccaacactg caatcagccg tcgtcgttta ctgcaaggcg cgggtgccat gtggctattg
    14521 agcgtaagtc aggtcagtct ggctgcggtc agccaggtcg tggcggtgcg cgtctggcct
    14581 gcgtccagct acacccgtgt gacggtagaa tcaaatcgtc agctgaaata taagcagttc
    14641 gcgttgagta accctgaacg cgtggtggtg gatatcgaag atgtaaacct gaactcggta
    14701 ctcaagggga tggcggcaca aattcgtgca gacgacccgt tcatcaagtc ggcgcgcgtc
    14761 gggcaatttg acccgcagac cgtacggatg gtttttgaat taaagcaaaa cgtaaaaccg
    14821 cagctgtttg cccttgcgcc ggtcgccggg tttaaagagc gtctggtgat ggacctctat
    14881 ccggccaatg cacaggatat gcaggacccg ctgctggcgc tgctggagga ttacaacaaa
    14941 ggcgacctcg aaaagcaggt gccgccagca caaagtggtc cacaaccggg taaagcaggg
    15001 cgcgatcgtc ctattgttat tatgcttgac cctggccacg gcggcgaaga ctccggtgcg
    15061 gtggggaaat acaaaacgcg cgaaaaagac gtggtattgc aaatagctcg ccgtctgcgc
    15121 tctctgatcg agaaagaggg caatatgaag gtgtacatga cgcgcaatga agacatcttc
    15181 attccgttgc aagtgcgcgt agcaaaagcc cagaaacagc gtgctgacct gtttgtctct
    15241 atccatgccg acgcctttac cagtcgtcag ccgagcggtt cctctgtgtt tgcgctctca
    15301 accaaaggtg caaccagtac tgcggcaaaa tatctggcac aaacccagaa cgcctcggac
    15361 ttgattggtg gcgtgagcaa aagcggtgac cgctatgtcg accacaccat gttcgatatg
    15421 gtgcagtcgc tgaccattgc cgacagcctg aagtttggta aagcggtact gaataagctc
    15481 ggtaaaatca acaagctgca taaaaatcaa gttgaacagg ccgggtttgc cgtactaaag
    15541 gcaccagata ttccctccat tctggtcgaa acggcgttta ttagtaacgt tgaggaagag
    15601 cgtaaactga aaacggcgac tttccagcag gaagttgcgg agtctattct tgcggggatt
    15661 aaagcgtatt ttgccgatgg ggcgacgctg gcgagaaggg gatgatgttt ttaatttatt
    15721 caatgaattt atgtttcatt gaataaatat tatttacata aatgttgaac gatggttttg
    15781 tgactgctgt cgaatatttg aatatcggct taaaaaataa gtctgtcatc tcttttatta
    15841 agcacaattc cgtgtaaggt atacgcatta gttaattatc ttgcaactgt taaatcaggc
    15901 aaggcaatgt ttgaagtaat tattacttct gacatgcctt gccttttttt tggggagcca
    15961 tggaatgatc atcgaaaaag tcatgaacaa taattgtgta caggcatcgg tgaacggaca
    16021 ggaggttatc atttctgggc ctggcgttgg ttacaataaa aatatggtat gtcggtccct
    16081 gagcatcctg caaaccggat ttttatgtca gaaatgagca aaaaaaataa actctacaaa
    16141 ttgattgagc atgtgggtat tgagtatatt tttgttgccg aaaagatagt gcattatgcg
    16201 gagagtaatc tcgaaaaaaa actcaatcca tcgctactac tgattcttgc tgatcacatt
    16261 tcgaatgcaa tatcccgagt cgtctcaggc atacaaatta ataatgtttt ccttgaggaa
    16321 atcaaagcgt tgtacaaagc agagtatgca ataagtcgtg atgcattaac aatcatta
//
LOCUS       DADLFA010000092        16340 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000092 DADLFA010000000
VERSION     DADLFA010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16340
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.092"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(206..1306)
                     /gene="chaA"
                     /locus_tag="JQH65_002433"
     CDS             complement(206..1306)
                     /gene="chaA"
                     /locus_tag="JQH65_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HAY6139820.1"
                     /translation="MSNAQEAVKTRHKEISLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            1576..1806
                     /gene="chaB"
                     /locus_tag="JQH65_002434"
     CDS             1576..1806
                     /gene="chaB"
                     /locus_tag="JQH65_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HAY6139821.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKDDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            1964..2659
                     /locus_tag="JQH65_002435"
     CDS             1964..2659
                     /locus_tag="JQH65_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HAY6139822.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGCTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(2703..3056)
                     /gene="ychN"
                     /locus_tag="JQH65_002436"
     CDS             complement(2703..3056)
                     /gene="ychN"
                     /locus_tag="JQH65_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HAY6139823.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            3240..4634
                     /gene="ychO"
                     /locus_tag="JQH65_002437"
     CDS             3240..4634
                     /gene="ychO"
                     /locus_tag="JQH65_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HAY6139824.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYFTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLLANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEYNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(4635..5285)
                     /gene="narL"
                     /locus_tag="JQH65_002438"
     CDS             complement(4635..5285)
                     /gene="narL"
                     /locus_tag="JQH65_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HAY6139825.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(5278..7074)
                     /gene="narX"
                     /locus_tag="JQH65_002439"
     CDS             complement(5278..7074)
                     /gene="narX"
                     /locus_tag="JQH65_002439"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HAY6139826.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDEQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPASSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            7413..8804
                     /gene="narK"
                     /locus_tag="JQH65_002440"
     CDS             7413..8804
                     /gene="narK"
                     /locus_tag="JQH65_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HAY6139827.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            9320..13042
                     /locus_tag="JQH65_002441"
     CDS             9320..13042
                     /locus_tag="JQH65_002441"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HAY6139828.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERAYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNQLMLTLGRGGPVVWLSEADAKELGIADNDWIEVYNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITGQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGND"
     gene            13060..14598
                     /gene="narH"
                     /locus_tag="JQH65_002442"
     CDS             13060..14598
                     /gene="narH"
                     /locus_tag="JQH65_002442"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HAY6139829.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            14595..15305
                     /gene="narJ"
                     /locus_tag="JQH65_002443"
     CDS             14595..15305
                     /gene="narJ"
                     /locus_tag="JQH65_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HAY6139830.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEVIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            15305..15982
                     /gene="narI"
                     /locus_tag="JQH65_002444"
     CDS             15305..15982
                     /gene="narI"
                     /locus_tag="JQH65_002444"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HAY6139831.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFAGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(16165..16296)
                     /locus_tag="JQH65_002445"
     ncRNA           complement(16165..16296)
                     /ncRNA_class="other"
                     /locus_tag="JQH65_002445"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
BASE COUNT         3924 a         4087 c         4384 g         3945 t
ORIGIN      
        1 ctcaattcat cttcacttca tccctgaaat gtttgcaata aagagtacat tccggctttt
       61 caacagctgt tgcagtggtt tcgtgcaggt tgtggtacag gctcgcaatt ctgattaacg
      121 agctgtccag gtagtatgaa gaaatacaat aagcaggaat ttatcttatc tcccccgatt
      181 tccgtcatcg gaggagatat aactgtcagg caaatatcgt catcaaatag gcggcaaaca
      241 gtgccagatg cgctgcgcca ttgagcacgt tagtacgtcc ggtggagaag gagatatggc
      301 acagcactaa agaggccacc atcaccacca tttctggcgc accaagtgca aactgtaatt
      361 cgttacccgt cataaaggca attagcgtga cgacaggtac ggtaagtgaa atggttgcta
      421 acactgaacc aaagaacaga ttcatcgcgc gctggacctg gttgttcaac actgctttta
      481 acgcacctaa accttccggc gacagaatca acagagccac caggaagcca gtaaaggcga
      541 caggggcatt catgctgtcg agcaatgtct ccagcgggct ggcattcatt ttggtgactg
      601 caataacggc aatcagatgg ataatcaacc agatagcatg ccacacgctg ctatgggcag
      661 acggtttacc gtgatgcggg tcgtcgtcat cactgtcatc ttcgtgctcg tagacaaaca
      721 aactttgatg cgttttggtc tggatcagca aaaatacgcc atacattgcc gcagaaatta
      781 atgctaccag taacgcctga ccggttgaaa aattcgccgc aggcagagcc attggaaata
      841 ccagtacgat tatcgccagg gggaacagcg caattaaata ctgcttgata ccaaacagat
      901 tcatatattg ggtggcaaac ttacgaccgc ccaacaataa tgaaaagcca accagcccac
      961 cggtaacaat cataatgatt gaatagagcg tatcacgcat tagcgttggc gcggcgtcgc
     1021 cggttgccat taaagctgaa atcaaactga cttcaagaat aaccactgaa aggctaagaa
     1081 taagcgaacc gtaaggttct cccaggcgat gggctaatac gtccgcatga cggacaacac
     1141 taaaggcgct acttaaaata ccaataagcg caagaagatt gatggcaatg accactggta
     1201 gtgtctggct gcttccccac aggaacagca ctaccagcgc cagaaccggg aaaataagcg
     1261 aaatctcctt gtggcgggtt tttaccgcct cttgagcatt tgacattatg gttatccctt
     1321 tgcagatgaa tttatcgaaa atgtaaaaaa taggtaggaa aaataacaga aagtgtctgg
     1381 atatcggtaa cattttacga atttttaccc cactgtcgat tttttactca ttgggacata
     1441 aaaataagta ttacgtttag acaatgtttg ttttagcgtt tcttaagaac agtctgacat
     1501 gaaaattctt atgttttggc aagagtagat attgttgacc acacttaatg ttcaactttg
     1561 taaaaggagt caacgatgcc gtataaaacg aaaagcgatc tgccggaaag cgtaaagcac
     1621 gttctaccgt ctcatgccca ggatatctat aaagaagcgt tcaacagcgc atgggatcaa
     1681 tataaagata aagacgatcg gcgtgatgac gccagtcgcg aagaaaccgc gcataaagtg
     1741 gcctgggctg ctgtgaagca tgaatatgcc aaaggggatg atgataaatg gcataaaaaa
     1801 tcgtaaaacc ggtagcttag ttaaagctat tcgtgcggtg ttgccttgca agtggtccgt
     1861 ggattgcata ttgtcccgtt agtggtttca aaatgagcag taaaaatgtc cggaagacac
     1921 caaaaagttg tcgcagggaa gtatgcagtg gcggaggtgt aaggtgataa cgcgtgattt
     1981 cttgatgaat gccgattgta aaacggcatt tggtgccatt gaagaatcac tcttatggtc
     2041 agcagagcaa cgagcggctt cgctggcggc gacgctggct tgtcgacctg atgagggacc
     2101 ggtgtggatc ttcggttatg gatcgttgat gtggaatccg gcactggagt ttaccgagtc
     2161 gtgcaccggt acactggttg gatggcatcg cgcgttttgc ctgcgcctga ccgccgggcg
     2221 cgggactgcg caccagccgg gacggatgct tgcactgaaa gagggcggac gcactacagg
     2281 cgtcgcctat cgactgccag aagagacgct ggagcaggaa ctaaccctgt tgtggaagcg
     2341 cgagatgatt accggctgtt atctgccaac ctggtgtcag cttgatcttg atgatggatg
     2401 cacagtaaac gccattgtgt ttattatgga cccgcgacat ccagaatatg aatctgacac
     2461 tcgcgctcag gtcatcgcgc cgttgattgc ggcggcgagc ggtccgctgg gaaccaacgc
     2521 acaatacctg ttttcactgg aacaggagct tatcaaactg ggaatgcagg acgatgggct
     2581 gaatgatttg ctggtatcgg taaaaaaact gctggcggag aattatccgg atggtgtgtt
     2641 acggccggga ttcgcctgag taaacttccc gcatagtggg gcgtcagacg ccccctcaaa
     2701 cattaaaatg tgagcacttt atcggctgac agcgtccatt gcgccagttc cacaagagta
     2761 ccgatttcca ccccatcaat cagaggaagt gtactaatcc cgcgcccgtc ggtacaggtt
     2821 ttgcacaatt tcaccggtac attctgagcg gtaaggatct ccagcatttg ctgaatgttg
     2881 tagccttccc ctggtttttg cccgcgcaac ccggctgtga ccgcatcaga catgaggaac
     2941 agacgcagat ccagattgct ctcctgctct cgtaacgcaa tggccagccg caagctgtta
     3001 aacaaggatt cgctcccgta aggtgcgcca ttggcaacga tcacgatttt ttgcattatt
     3061 tactcctgta ttcagggaat tagacactca tcttctatct tactgcttct gcagcgtctg
     3121 accaatcggt cacattttta aggattttcc tgaaagcgcg agaaaatacg acaaaagttg
     3181 ccagtaatcg ttattcttta aggctatggt tttcattatt accggaagtt accgacgttt
     3241 tgagccgttt cgttcctcgc attatcccgt tttatttact cttgcttgta gcaggcggta
     3301 cagctaacgc acaatctacc ttcgagcaaa aagcggcaaa tccctttgat aataacaatg
     3361 atggtctgcc ggatttaggc atggctcccg aaaatcatga tggggaaaaa cactttgctg
     3421 aaattgtgaa agatttcggc gaaaccagta tgaatgataa cgggctggat actggcgagc
     3481 aggcaaaagc tttcgcattg ggaaaagtcc gcgacgcgct tagtcaacag gttaatcagc
     3541 acgtagagtc ctggctatca ccgtggggaa atgccagtgt tgacgtcaaa gtggataacg
     3601 aaggacattt caccggcagt cgtggaagct ggtttgtgcc gttacaagat aatgatcgtt
     3661 atttcacctg gagccagctt ggtcttactc agcaggataa tgggttggtg agcaatgtgg
     3721 gcgttgggca acgctgggcg cgcggcaact ggctggtggg ttataacact ttttatgaca
     3781 acttgctgga cgaaaatctt cagcgagcgg gctttggtgc cgaagcgtgg ggcgaatatt
     3841 tgcgattatt ggcaaacttt tatcagccgt ttgctgcatg gcatgaacag acagccacgc
     3901 aggaacaacg gatggcgcgc gggtacgacc tgacagctcg gatgcgcatg ccgttctatc
     3961 aacacctcaa taccagtgtc agcctagaac agtattttgg tgatcgtgtt gatttgttta
     4021 actctggtac gggttatcac aatcccgtcg cgttgagtct gggattaaat tacacccctg
     4081 tgccattagt cactgtgacg gcccagcata aacagggtga aagtggcgag aatcaaaata
     4141 acctcgggct gaatcttaat taccgctttg gtgtaccgct caaaaaacaa ctttctgcgg
     4201 gcgaggttgc cgaaagtcag tcgttacgtg gtagtcgcta tgacaatccg cagcgaaata
     4261 atctaccgac tcttgagtac cgacagcgaa aaacgttaac ggtgtttctg gcgacaccgc
     4321 cgtgggatct aaaacctggc gaaacagtgc cgctgaaatt acaaatccgc agtcgttacg
     4381 gtattcggca actgatttgg cagggcgata cgcagatatt aagtttgacg ccgggcgcac
     4441 aagccaacag cgcggagggc tggacgctga tcatgcctga ctggcagaac ggggaagggg
     4501 caagcaatca ctggcgattg tcggtggtgg tggaatataa ccaggggcag cgtgtctcct
     4561 ccaatgagat cacgctaacg cttgtcgaac cgttcgatgc attgtcaaac gacgaactgc
     4621 gctgggaacc gtaatcagaa aatgcgctcc tgatgcaccc ataccgctgc ttccacgcga
     4681 gacttgagct tcattttctt cagcatgtgc ttgacgtgca cttttactgt gctttcggtg
     4741 atatccaggc ggcgggcaat catcttgttc ggcaaaccct gggcaatcag cttgagaata
     4801 tcgcgctcgc gtggggttaa ctggttaaca tcgcgctcag tagtggcacg gttagcgcgc
     4861 aagctggcgg ccagaacagg cgttaatgct tcgcttaata ccatttcgcc agcagctgcc
     4921 tgatgcaatg ctttcagcag atcttccggt tccatatctt ttaacagata accatccgcg
     4981 ccgcgtttca gtgcggtgac cacatcttct tcatggttag agacgctgaa taccacaatg
     5041 cgccctgaga gggacttttc gcgcagttta tccagcgttt ccagaccgtt catgccgggc
     5101 atattgagat ctaacaggat cagatcggga tcaagagact ccgccagttc aataccctgt
     5161 tcgccattac tcgcttcgcc aaccacggtg atatctggtg ccatactgat aagctgtttt
     5221 acgccagttc gcagcatcgg gtgatcgtcg atcagcagga tagtagccgg ttcctgatta
     5281 ctcatgggta tctccttgga cgtctgtgaa agttttttcg ggaataaagg tgaccaccac
     5341 ttcggtgcca cctgattcac gacggcggac gcggcaatcg cctcgtaaac tttgcgcacg
     5401 gtcgcgcatt attatcatgc cgtagtgatt gctgcggatg gcattttcag gcacgccgca
     5461 gccgttatct tggacggtca gtttgacctg attatcgttt tgcgccaccg tcaccacgac
     5521 ctcactcgct tgcgaatgtt tgagggcgtt acttaatgcc tcacgggcaa tttgcaacaa
     5581 gtggattgcc tgatgtgaag gcaccagacg aggcggcaat tgataatcca gcttcaccgg
     5641 gaagccaaat ttggcgctgt actcttcgca actcgcctcc agcgccggac gtaatccagg
     5701 ctcggtgagc tgcaagcgga atgtggtgag caattcacgc aactgcgccc aggatgcatt
     5761 cagttcgtta cggatctgac ttaacagttc gcggctgctt gctggcagcg catcgccctg
     5821 catctgtaaa caactcacct gcatcttcat gcaagagaga gattgggcaa tagaatcatg
     5881 cagttcgcgc gcaatggtgg cacgctcttc catcacgatc aactgttgct gacgttcctg
     5941 atggcgatcc agcgccagcg tggcggtgag ttgttcaacc agggtatcca ccagttgttg
     6001 ttgatcatgg ctaagatgac gcccctgcgg cagggtcgcc agcaaaatac cgtactgcgt
     6061 atgagagtca gccagccgcc acttcagggt tgtgccgcga tcaccaacgg gtaatacgcc
     6121 gcgcgggcag agctggcagc ctttatcatc acaagtcata tctggctggc aggtaaactc
     6181 ctgatgattc tcttcatcat cagtgtcata cacccgcagt tcgatatcac gtagcagggt
     6241 taaattctgt aagccgttga gtacaggtga caggcgttca cacagcgggg cgcgggaatg
     6301 caaacggcgg ttagcctgcc ataaaaaaga gaggatctga tttttatgtt ccagcccggc
     6361 ggttttctcc tgaacccgct gctcaagtac ggcataactt tcggccagtt ctgcagacat
     6421 attgttcaat gcagttccaa gcatcgccat ttcgttgcgc ccgctgatgt tcgcgcgttg
     6481 ggtaaaatcg cgatgactga cggcactcgc cattgccagc agttgccgcc acggttgtag
     6541 cagtcgcgcc cgcaaccaga taatagtgaa caccagtaaa agtgccataa ataccgccat
     6601 tacccgatgg accagtacca ctgtctcaat gcgcatttcc gtggtgcggt caaaaccaga
     6661 taccagctga tcaagcccgg caacaaactg gctgacatcc gctgacaccg tttcgcggtt
     6721 ttgtgcacgc atcagtgcag ggatcagttc attacgccag taatcttgta aaccctgtaa
     6781 ttgcgccagt tgttcgtctc gttctgctgc tcgagttaac tcggcgctaa atgccgtttg
     6841 ttccatctct ttaattaagg gcttgtcttt ctcacttaat ggcactgccg ccaacagacg
     6901 gtaactttgc atgcgcagcg atcccgcttt gttgatcgca tgggcgctgc cctgaacgcc
     6961 ttgcaccagc cagccagaaa ccgccatccc tgccagtcca atagcagtag aaagcaacac
     7021 aataagcgca acctgattaa ccagggtgag cggagagaga caacgtttaa gcatgtaaac
     7081 ctcttccttc aggctttaaa tgagcaataa ccttaatgaa tgtgacgata cattctggaa
     7141 tggcagtatt ctcggctatt ggctgaagta tacccatacc cggaaagagt tactccttat
     7201 ttgccgtgtg gttagtcgct ttacatcggt aagggtaggg attttacagc accgtgaaaa
     7261 atctcataat ttttatgaaa tcactgtact cactatgggt aatgataaat atcaatgata
     7321 gataaagtta tcttatcgtt tgatttacat caaattgcct ttagctacag acactaaggt
     7381 ggcagacatc gaaacgagta tcagaggtgt ctatgagtca ctcatccgcc cccgaaaggg
     7441 ctactggagc tgtcattaca gattggcgac cggaagatcc tgcgttctgg caacaacgcg
     7501 gtcaacgtat tgccagccgt aacctgtgga tttccgttcc ctgtctgctg ctggcgtttt
     7561 gcgtatggat gttgttcagc gctgttgcgg tgaacttacc gaaagtcggt tttaatttta
     7621 cgaccgatca gctatttatg ttgactgcgc tgccttcggt ttctggcgcg ttattacgtg
     7681 ttccatactc ctttatggtt cctatcttcg gtggtcgtcg ctggacggcg ttcagcaccg
     7741 gtattctgat tattccttgc gtctggctgg gttttgccgt gcaggatacc tccacgcctt
     7801 atagcgtctt catcatcatc tctctgctat gcggctttgc tggcgcgaac ttcgcatcca
     7861 gtatggcaaa catcagcttc ttctttccga aacagaagca gggtggcgcg ctgggtctga
     7921 atggtggtct gggaaacatg ggcgtcagcg tcatgcagtt ggttgctccg ctggtggtat
     7981 cactgtcgat tttcgcagta tttggtagcc agggcgtcaa acagccggat gggactgagc
     8041 tgtatctggc gaatgcgtcc tggatatggg tgccgttcct tgccatcttc accattgcgg
     8101 cgtggtttgg catgaacgat cttgctacct cgaaagcctc catcaaggag cagttgccgg
     8161 tactcaaacg gggtcatctg tggattatga gcctgctgta tctggcaacc ttcggctcct
     8221 tcatcggctt ctccgcgggc tttgcaatgc tgtcaaaaac gcagttcccg gatgttcaga
     8281 ttctgcaata cgctttcttc gggccgttta ttggtgcgct ggcgcgttct gcaggtggtg
     8341 cattatctga ccgtctgggc ggaactcgtg tcacgctggt gaactttatt ctgatggcga
     8401 ttttcagcgg cctgctgttc ctgaccttac cgactgacgg gcagggcgga agcttcatgg
     8461 cgttcttcgc ggtcttcctg gcgctgttcc tgacagctgg gctgggtagt ggttccactt
     8521 tccagatgat ttcagtgatc ttccgtaaac tgacaatgga tcgcgtgaaa gcagaagggg
     8581 gttctgacga acgtgcgatg cgtgaagcgg caaccgacac ggcggcggcg ctgggtttca
     8641 tctctgcgat tggcgcgatt ggtggcttct ttatcccgaa agcgtttggt agctcgctgg
     8701 cattaacggg ttcgccagtc ggcgcaatga aggtattttt gattttctat atcgcctgcg
     8761 tagtgattac ctgggcggta tatggtcggc attctaaaaa ataaaccgtt actcgtcata
     8821 ctccgggtta catgtgctgc ggctgcgttc attcacccca gtcacttact ttagtaagct
     8881 cctgggattc attcacttgc cgccttcctg taaaccgaat tatatagagt aaaatatttg
     8941 attatccttt gcgcggcatg atgtcgcgct ttttttaagc gttatttagt tacaacatac
     9001 taatgttata tggtttattt cgccggattt cattaagagc cattaatatg ttacccatgg
     9061 ggaatactcc ttaataccca tctgcataaa aatcttaata gtttaaataa ctacaggtat
     9121 aaaacgtctt aatttacagt ctgttatgtg gtggctgttg attatcctaa aggggtatct
     9181 taggaattta ctttattttt catccccatc actcttgatc gttatcaatt cccacgctgt
     9241 ttcagagcgt taccttgccc ttaaacatta gcaatgtcga tttatcagag ggccgacagg
     9301 ctcccacagg agaaaaccga tgagtaaatt cctggaccgg tttcgctact tcaaacagaa
     9361 gggtgaaacc tttgccgatg ggcatggcca gcttctcaat accaaccgtg actgggagga
     9421 tggatatcgc cagcgttggc agcatgacaa aatcgtccgc tctacccacg gggtaaactg
     9481 caccggctcc tgcagctgga aaatctacgt caaaaacggt ctggtcacct gggaaaccca
     9541 gcagactgac tatccgcgta cccgtccgga tctgccaaac catgaacctc gcggctgccc
     9601 gcgcggtgcc agctactcct ggtatcttta cagtgccaac cgcctgaaat acccgatgat
     9661 gcgcaaacgc ctgatgaaaa tgtggcgtga agcgaaggcg ctgcatagcg atccggttga
     9721 ggcatgggct tctatcattg aagacgccga taaagcgaaa agctttaagc aggcgcgtgg
     9781 acgcggtgga tttgttcgtt cttcctggca ggaggtgaac gaactgatcg ccgcatctaa
     9841 cgtttacacc atcaaaaact acggcccgga ccgtgttgct ggtttctcgc caattccggc
     9901 aatgtcgatg gtttcttacg catcgggtgc acgctatctc tcgctgattg gcggtacttg
     9961 cttaagcttc tacgactggt actgcgacct gcctcctgcg tctccgcaaa cctggggcga
    10021 gcaaactgac gtaccggaat ctgctgactg gtataactcc agctacatca tcgcctgggg
    10081 ttcaaacgtg ccgcagacgc gtaccccgga tgctcacttc tttactgaag tgcgttacaa
    10141 aggcaccaaa actgttgccg tcacaccaga ctacgctgaa atcgccaaac tgtgcgatct
    10201 gtggctggca ccgaaacagg gcaccgatgc ggcaatggcg ctggcgatgg gccacgtaat
    10261 gctgcgtgaa ttccacctcg acaacccaag ccagtatttc accgactatg tgcgtcgcta
    10321 caccgacatg ccgatgctgg tgatgctgga agaacgcgac ggttactacg ctgcaggtcg
    10381 tatgctgcgc gctgctgatc tggttgatgc gctgggccag gaaaacaatc cggaatggaa
    10441 aactgtcgcc tttaatacca atggcgaaat ggttgcgccg aacggttcta ttggcttccg
    10501 ctggggcgag aagggcaaat ggaatcttga gcagcgcgac ggcaaaactg gcgaagaaac
    10561 cgagctgcaa ctgagcctgc tgggtagcca agatgagatc gctgaggtag gcttcccgta
    10621 ctttggtggc gacggcacgg aacacttcaa caaagtggaa ctggaaaacg tgctgctgca
    10681 caaactgccg gtgaaacgcc tgcaactggc tgatggcagc accgccctgg tgaccaccgt
    10741 ttatgatctg acgctggcaa actacggtct ggaacgtggc ctgaacgacg ttaactgtgc
    10801 aaccagctat gacgatgtga aagcttatac cccggcctgg gccgagcaga ttaccggcgt
    10861 ttctcgcagc cagattattc gcatcgcccg tgaatttgcc gataacgctg ataaaacgca
    10921 cggtcgttcg atgattatcg tcggtgcggg gctgaaccac tggtatcacc tcgatatgaa
    10981 ctatcgtggt ctgatcaaca tgctgatttt ctgcggctgt gtcggtcaga gcgggggcgg
    11041 ctgggcgcac tatgtaggtc aggaaaaact gcgtccgcaa accggctggc agccgctggc
    11101 gtttgccctt gactggcagc gtccggcgcg tcacatgaac agcacttctt atttctataa
    11161 ccactccagc cagtggcgtt atgaaaccgt cacggcggaa gagttgctgt caccgatggc
    11221 ggacaaatcc cgctataccg gacacttgat cgactttaac gtccgtgcgg aacgcatggg
    11281 ctggctgccg tctgcaccgc agttaggcac taacccgctg actatcgctg gcgaagcgga
    11341 aaaagccggg atgaatccgg tggactatac ggtgaaatcc ctgaaagagg gttccatccg
    11401 ttttgcggca gaacaaccag aaaacggtaa aaaccacccg cgcaacctgt tcatctggcg
    11461 ttctaacctg ctcggttctt ccggtaaagg tcatgagttt atgctcaagt acctgctggg
    11521 gacggagcac ggtatccagg gtaaagatct ggggcaacag ggcggcgtga agccggaaga
    11581 agtggactgg caggacaatg gtctggaagg caagctggat ctggtggtta cgctggactt
    11641 ccgtctgtcg agcacctgtc tctattccga catcattttg ccgacggcga cctggtacga
    11701 aaaagacgac atgaatactt cggatatgca tccgtttatt cacccgctgt ctgcggcggt
    11761 cgatccggcc tgggaagcga aaagcgactg ggaaatctac aaagccatcg cgaagaaatt
    11821 ctccgaagtg tgcgtcggcc atctgggtaa agaaaccgac atcgtcacgc tgcctatcca
    11881 gcatgactct gccgctgaac tggcgcagcc gctggatgtg aaagactgga aaaaaggcga
    11941 gtgcgacctg atcccaggta aaaccgcgcc acacattatg gtcgtagagc gcgcttatcc
    12001 ggcgacttac gaacgcttta cctctatcgg cccgctgatg gagaaaatcg gtaatggcgg
    12061 taaagggatt gcctggaaca cccagagcga gatggatctg ctgcgtaagc tcaactacac
    12121 caaagcggaa ggtccggcga aaggccagcc gatgctgaac accgcaattg atgcggcaga
    12181 gatgatcctg acactggcac cggaaaccaa cggtcaggta gccgtgaaag cctgggctgc
    12241 cctgagcgaa tttaccggtc gtgaccatac gcatctggcg ctgaataaag aagacgagaa
    12301 gatccgcttc cgcgatattc aggcacagcc gcgcaaaatt atctccagcc cgacctggtc
    12361 tggtctggaa gatgaacacg tttcttacaa cgccggttac accaacgttc acgagctgat
    12421 cccatggcgt acgctctctg gtcgtcagca actgtatcag gatcaccagt ggatgcgtga
    12481 tttcggtgaa agcctgctgg tttatcgtcc gccgatcgac acccgttcgg tgaaagaagt
    12541 gataggccag aaatccaacg gcaacccgga aaaagcgctc aacttcctga cgccgcacca
    12601 gaagtggggg atccactcca cctacagcga caaccagttg atgctgacct taggtcgcgg
    12661 tggcccggtg gtctggctga gcgaagccga tgccaaagag ctgggcattg ccgataacga
    12721 ctggattgaa gtttacaaca gcaacggtgc gctgactgcc cgtgcggttg tcagccagcg
    12781 tgttccggca gggatgacca tgatgtacca cgcgcaggaa cgtatcgtta acctgccggg
    12841 ttcggaaatt actggccagc gtggggggat ccacaactcg gtcacccgta tcacgccgaa
    12901 accgacgcat atgattggtg gctatgccca tctggcatac ggctttaact actacggcac
    12961 cgtaggttct aaccgcgatg agtttgttgt agtgcgtaag atgaagaaca ttgactggtt
    13021 agatggcgaa ggcaatgact aggtacagga gagcgtaaaa tgaaaattcg ttcacaagtc
    13081 ggcatggtgc tgaatctcga taagtgcatc ggctgccaca cctgttcagt tacctgtaaa
    13141 aacgtctgga ccagccgtga aggcgtggag tacgcgtggt tcaacaacgt ggaaaccaag
    13201 ccgggtcagg gcttcccgac tgactgggaa aaccaggaaa aatacaaagg cggctggatc
    13261 cgtaaaatca acggcaaact gcagccgcgc atgggtaacc gtgccatgtt gctgggtaaa
    13321 atcttcgcta acccgcatct gccggggatc gacgattatt acgagccgtt cgattttgac
    13381 tatcagaacc tgcataccgc gccggaaggc agcaaatcgc agccgattgc ccgtccccgt
    13441 tcgctgatta ccggggaacg gatggcgaaa atcgaaaaag ggccgaactg ggaagatgac
    13501 ctgggtggtg agtttgacaa actggcgaaa gacaagaact tcgacaacat ccagaaggcg
    13561 atgtatagcc agttcgaaaa cacctttatg atgtatttgc cgcgcctgtg cgaacactgc
    13621 ctgaacccgg catgtgtggc gacctgcccg agcggtgcga tttacaagcg tgaagaagat
    13681 ggcatcgtcc tgatcgacca ggataaatgc cgtggctggc gtatgtgcat caccggatgc
    13741 ccgtacaaaa aaatctactt caactggaag agcggtaagt ctgagaagtg catcttctgc
    13801 tatccgcgta ttgaagcggg tcagccgacc gtgtgctcag aaacctgtgt cggtcgtatc
    13861 cgttatcttg gcgtgctgct gtacgatgcc gacgcgattg aacgtgcagc cagcaccgag
    13921 aacgagaaag atctttacca gcgtcagctg gaggtgttcc tcgatccgaa cgatccgaaa
    13981 gtcatcgagc aggcgattaa agacggtatt ccgctgagcg ttattgaagc cgcacagcag
    14041 tcgccggttt ataaaatggc aatggaatgg aaactggcgc tgccgctgca tccagaatat
    14101 cgcacactgc cgatggtctg gtacgtgccg cctctgtctc cgattcagtc tgcagcagac
    14161 gcgggtgagc tgggtagcaa cggcattctg ccagacgtcg aaagcttgcg tattccggta
    14221 cagtatctgg cgaatctgct gaccgccggt gataccaaac cggtactgcg cgcactgaaa
    14281 cgtatgctgg cgatgcgtca ttacaaacgt gctgaaaccg ttgacggtaa agttgatacc
    14341 cgtgcgctgg aagaggtcgg tctgaccgaa gcccaggcac aggagatgta ccgttatctg
    14401 gctattgcta actacgaaga tcgctttgtg gtgccgagta gtcatcgtga actggcacgg
    14461 gaagccttcc cggagaaaaa tggctgcggc tttacctttg gtgatggctg ccacggctca
    14521 gataccaaat tcaatctgtt caacagccgt cgtatcgatg ccatcgatgt gaccagcaaa
    14581 acggagccgc atccatgatc gaactcgtga ttgtatcgcg tctccttgaa tatccggatg
    14641 ctgccttatg gcagcatcaa caagagatgt ttgaggtgat tgctgcgtcg aaaaatctgt
    14701 cgaaagaaga tgcccatgcg ctgggcattt tcctgcgcga tttaacggcg atggatccgc
    14761 tcgatgccca ggcccagtac agcgaactgt tcgaccgtgg ccgcgccacg tcactgttgc
    14821 tatttgaaca tgttcacggt gaatcccgtg accgcggcca ggcgatggtg gacctgctgg
    14881 cgcagtacga gcagcacggc ttgcagttaa acagccgcga attaccggac catctgccgc
    14941 tgtatctgga gtacctgtcg cagctgccgc aaagcgaagc cgtggaaggt ttgaaagata
    15001 tcgcgccgat tctggcattg ctgagcgcgc gtctgcaaca gcgtgaaagc cgttatgccg
    15061 tgatgtttga tctgctgctg aaattggcca ataccgctat cgacagcgac aaagtggcgg
    15121 aaaaaattgc cgacgaagcg cgcgatgata cgccgcaggc gctggatgct gtctgggaag
    15181 aagagcaggt taaattcttt gctgacaaag gctgcggtga ttcagcaatc actgctcatc
    15241 agcgtcgctt tgccggtgcc gtcgcgccgc aatatctgaa tatcaccacc ggaggacagc
    15301 actaatgcaa ttcctgaata tgttcttctt tgatatctac ccgtacatag ccggggcggt
    15361 cttcctgatt ggtagctggc tgcgttatga ctacgggcag tacacctggc gcgcggcgtc
    15421 cagccagatg ctggatcgca aagggatgaa cctggcgtcg aacctgttcc atatcggtat
    15481 tctggggatt tttgctggtc acttcttcgg tatgctgacg ccgcactgga tgtatgaagc
    15541 ctggctgccg attgaagtga aacagaaaat ggcgatgttt gctggtggtg ccagcggcgt
    15601 gctgtgtctg attggcggcg tgctgttgct gaagcgtcgt ctgttcagcc cacgcgtgcg
    15661 tgcaaccact accggagcgg atatcctgat cctgtcgctg ctcgttatcc agtgcgcgct
    15721 gggcctgttg accattccgt tctccgctca gcatatggac ggtagcgaga tgatgaaact
    15781 ggttggctgg gcgcagtcgg tggtgacctt ccacggtggc gcttctcaac acctcgatgg
    15841 tgtggcgttt atcttccgtc tgcacctggt gctggggatg acgttattcc tgctgttccc
    15901 gttctcgcgt ctggtacaca tctggagcgt accggtggag tatctgacac gtaagtacca
    15961 gctggtgcgc gctcgtcact aaacgaattt tagttcacat agaccctgct tcggcggggt
    16021 tttttatggg cacggtgcgg ggtgagttgt cggatgcgct tcgcttatcc gacctacagg
    16081 ggaggatatt gtaggcccgg taaacgtggt gccatcgggc aaaattagct cggagtaaca
    16141 ggttttgatt attcaaggaa gcgatggtgg tgggggaagg attactcagc gctgcgcgct
    16201 tcgcccttcg ggtcgttgcc tgcggcaacg ctctctcgct ggcgctcaag tcgaaccttg
    16261 gtcgaagctt ctcatccttc cccgcttggg cagaatattt aattgcggat tcgtttgaga
    16321 gtttacggac ttttgaaaag
//
LOCUS       DADLFA010000093        16113 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000093 DADLFA010000000
VERSION     DADLFA010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16113
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.093"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..321)
                     /locus_tag="JQH65_002446"
     CDS             complement(<1..321)
                     /locus_tag="JQH65_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HAY6139832.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVR"
     gene            complement(326..511)
                     /locus_tag="JQH65_002447"
     CDS             complement(326..511)
                     /locus_tag="JQH65_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HAY6139833.1"
                     /translation="MKILLAALTSSFMLAACTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLY"
     gene            complement(508..3147)
                     /locus_tag="JQH65_002448"
     CDS             complement(508..3147)
                     /locus_tag="JQH65_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HAY6139834.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVIAFEYQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTNINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3355..4344
                     /gene="ldhA"
                     /locus_tag="JQH65_002449"
     CDS             3355..4344
                     /gene="ldhA"
                     /locus_tag="JQH65_002449"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HAY6139835.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPANYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4455..4877
                     /gene="hslJ"
                     /locus_tag="JQH65_002450"
     CDS             4455..4877
                     /gene="hslJ"
                     /locus_tag="JQH65_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HAY6139836.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(4874..5140)
                     /locus_tag="JQH65_002451"
     CDS             complement(4874..5140)
                     /locus_tag="JQH65_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HAY6139837.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5414..8938
                     /gene="nifJ"
                     /locus_tag="JQH65_002452"
     CDS             5414..8938
                     /gene="nifJ"
                     /locus_tag="JQH65_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAY6139838.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DSYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGKRETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTQPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVEGNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            9304..10437
                     /gene="ompN"
                     /locus_tag="JQH65_002453"
     CDS             9304..10437
                     /gene="ompN"
                     /locus_tag="JQH65_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HAY6139839.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGFSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            10578..11012
                     /gene="uspF"
                     /locus_tag="JQH65_002454"
     CDS             10578..11012
                     /gene="uspF"
                     /locus_tag="JQH65_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HAY6139840.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            11595..12485
                     /gene="sitA"
                     /locus_tag="JQH65_002455"
     CDS             11595..12485
                     /gene="sitA"
                     /locus_tag="JQH65_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HAY6139841.1"
                     /translation="MLLGCLALTCSIAFQASATEKFKVITTFTIIADMAKNVAGDAAE
                     VSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNGVPEVIVSS
                     GVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQRNADTYKA
                     KITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINADQQGTPQQV
                     RKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTENGPVPTYID
                     LLKVTTSTLVQGIKAGKREK"
     gene            12485..13312
                     /gene="sitB"
                     /locus_tag="JQH65_002456"
     CDS             12485..13312
                     /gene="sitB"
                     /locus_tag="JQH65_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HAY6139842.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGYMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            13309..14166
                     /gene="sitC"
                     /locus_tag="JQH65_002457"
     CDS             13309..14166
                     /gene="sitC"
                     /locus_tag="JQH65_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HAY6139843.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            14163..15020
                     /gene="sitD"
                     /locus_tag="JQH65_002458"
     CDS             14163..15020
                     /gene="sitD"
                     /locus_tag="JQH65_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HAY6139844.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(15338..>16113)
                     /locus_tag="JQH65_002459"
     CDS             complement(15338..>16113)
                     /locus_tag="JQH65_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66-like element ISSfl3 family transposase"
                     /protein_id="HAY6139845.1"
                     /translation="APGNGKTKTGRLWVYVRDDRNAGSSLPAAVWFAYSADRKGEHPQ
                     LHLAKYQGVLQADAYAGYNVLYETGRVKEAGCLAHARRKIHDEDVRRPTEMTQEALRR
                     IAELYDIEAEIRGSPAEERLAVRKARSVQLMQSLYDWIQLQRKTLSKHAEMAKAFDYI
                     LNHWNALNEFCRDGWVEIDNNIGENALRSVAVGRKNYLFFGSDKGGESAAIIYSLLVT
                     CKQNEVEPEDWLREVIEKLNDWPSNQVHELLPWNFSSVK"
BASE COUNT         4000 a         4096 c         3890 g         4127 t
ORIGIN      
        1 tcgtacccat ttaccgttta tcccttgtac atattccccc ggtttggccc gagctacgag
       61 cttttgcccg gcgagtttcg cgatatcatc tacagacaca ttattttgct ttgccagttg
      121 ttggtaactc gctttacgtt cggcattaat gtcggttact aatttctctg tttcagcatc
      181 cgttttcagc gcaaccagat aaccataaaa tgtttccccg acccgcccct gcgttctggc
      241 ttcatccaga gtcaatgcca ttacattgct gcttaccagc ccaacaagaa acgcacaaag
      301 aagtaatgtt ttcttcatca tcgcctcagt aaagatcgct acgagtttca agcagctctt
      361 cgacatcttt gtctgccttg atgatgatct catgctcaat tttaacgttc atattgatag
      421 tgatcggttc cttgggcgca gcgacttcaa tacgcggcgt acaggcagcc agcataaaag
      481 atgacgtcaa cgcagccagt aaaattttca ttgtttttcc tcacactctt ttccttgcgg
      541 acagtcattt cccggcagac gtgcgttctg ctccaaccat gcctggagat tatcgccaaa
      601 gcgtaaactg cgccacagcg taaaaatatt ctcttcatga tggtaattaa ggtttaccgt
      661 accgctttta ccatcaacgc gactggtacc tgtaatgttg gcctgcatgg ttaataaacc
      721 gagattatct aaattaatat ttgtcgatga acggctaatt tccatatagc gcaaccagtt
      781 aattgctgaa cccgcagtca tattgtcttt caccaccgca tctgcggtgt ctttatccag
      841 ccgcaaggtc attggcccgc tatttgccag ccaaccatct ttcactatcc atttttcgtt
      901 gtttagccat aacggcaatg caccactaaa tgccccggac atggcgaatt gtttcggatt
      961 gacggcgcta accagttcgc tggatgagag attattcagc cgcaacagcg ccgggtcatg
     1021 ttgcggcata cgtaattgtt tcatcagtac gttaccgcct aacacatcga cgctaacatc
     1081 agtcaacagc aagggttctt cttcagtcca cggataacgc ccttgcaaat cagccgtaat
     1141 atttttcgct gtcaccagat taatcacttc ggcaattcgc aacgtaacgg ggccgcgagt
     1201 ccccagatgc caggctccat cggcaaaacg gaaaggcagg acaaaatcga caccgttaac
     1261 ctgattatct ggcatccagg cactaccgcc tttcaacacg ccgtgccctc ccgcgcggaa
     1321 tccttgttca ggcgcagcag aaaatgcaac ctgagcatac agttcaccat cgcgtaagtt
     1381 catcttccag tcgggtggca ccagcggctg gaatacagtc agtgattgtt ttggccacca
     1441 ggcgttaccg cgcagacgaa taccgtccca gcgaccattt acccgaaccg ggccaatctc
     1501 accagcatgt aaatcgcctt taaagaggaa atacgtcgga tcgcgcccat cgacgctaaa
     1561 ttttaaggtt gatggcggta acacactgcc gccagtgaac agcgtttgcc cggcgtccag
     1621 tgacagcgcg ccgctgaagg agggatgctg tgcgtcacgt acccagacga cgggcttgtc
     1681 cagaattaat cgcggctttt ctaccgtcat cgtaccgtat tgtaactggt cgaaaccggt
     1741 ggagaggtcg gtcagcgtaa tcgtgctgtc atgccattca ccttttcctg cgacatccca
     1801 ggtggcattc atcggtgtaa aactgccttt tccccagtag cgccactgcc agcggcccgc
     1861 gtcagggaga aaatcattcg ccagcccatc catatgcagc acgaaatcgc ccagttcatt
     1921 ttcatgcgcc tgcaaaatgg cctgcaaacg tccgtcaaca ccacgttggg tgacttttac
     1981 acccgctaaa ggccagcgga tttcatcgat gtccagcgaa tcgatgactc ttccctttga
     2041 acgaagtaac gcgcctggct caaaggccag cgttgggtca gtcagacttc cacttagctg
     2101 cgcgggtaag cgggcatata aaatgaggtc cgcctgtttc gcttcaccgg tcagctgcag
     2161 aggcagctgg ctgttatcca tgcttaattt tcctgggcca aaatttagca ccgcgttgcc
     2221 tttacccgct tgcccctggg tcagcacact cagtcgtccg ctgatcagag cgttctcaag
     2281 ccctgcctgc cagttgtcga ctttgacacc cagtcggcca ctcaaaggaa aacctgcata
     2341 cggccaactc cagcgaccat cgcttacggt caattgttga cgagtaattt gccacggcaa
     2401 atcgagcaac ggatcgccgt tatcacgtgc cagcacaatc aattgcccgc tattttcctg
     2461 ccagtccagc tctgcatcca ccagtgacgg ttcctgcggc aagtttaacg tcgcagtagc
     2521 atgcccactt acaggaagtc catccggcac gagcggcata gtaaattccc ccaccagttt
     2581 aaccggcggc tgatattcaa acgcgatgac atccagttcg ctgactgtaa gttgttgccc
     2641 tttcagctgg ccttgaaatt taactttttc gccctgataa cgcagttgct ggatatcaga
     2701 ggttaatgcg agagagagtt ttccctgcca ttcctgccag ggagaaaaaa tcagtttatc
     2761 gatattgatc caggtgttag gcagcatgga ctgccactgc gcgagggttt ttggagcagc
     2821 tggcgattgc tccgtctgcg gcaatttcgc cagacaagca gaatcaagtt ctaccgtgcc
     2881 gacgttcaat aaccagcggc tgggatgtga aaggctggcg ttggtgatat gtgcaagctg
     2941 acaatctccc accagataac ggagatcggg aatgattaaa cctttacgcg taatgcgtgg
     3001 gctttcatct aatgcaatac gtgtcccgag cggtagccag atgcccgcca gcgtgggaac
     3061 ccacagcccg agcgtcatca gcagcgtcaa cggcacaaga ataatcagta ataacagcgc
     3121 gagaacggct ttatatttac ccagcatggg tagttaatat cctgatttag cgaaaaatta
     3181 agcattcaat acgggtattg tggcatgttt aaccgttcag ttgaaggttg cgcctacact
     3241 aagcatagtt gttgatgaat ttttcaatat cgccatggtt ttcaattata tttgaaattt
     3301 tgtaaaatat ttttagtggc ttaaatgtga ttcaacatca ctggagaaag tcttatgaaa
     3361 ctcgccgttt atagcacaaa acagtacgac aagaagtacc tgcaacaggt gaacgagtcc
     3421 tttggctttg agctggaatt ttttgacttt ctgctgacgg aaaaaaccgc taaaactgcc
     3481 aatggctgcg aagcggtatg tattttcgta aacgatgacg gcagccgccc ggtgctggaa
     3541 gagctgaaaa agcacggcgt taaatatatc gccctgcgct gtgccggttt caataacgtc
     3601 gaccttgatg cggcaaaaga actggggctg aaagtagtcc gtgttccagc ctatgatcca
     3661 gaggccgttg ctgaacacgc catcggtatg atgatgacgc tgaaccgccg tattcaccgc
     3721 gcgtatcagc gtacccgtga cgctaacttc tctctggaag gtctgaccgg ctttactatg
     3781 tatggcaaaa cggcaggcgt tatcggtacc ggtaaaatcg gtgtggcgat gctgcgcatt
     3841 ctgaaaggtt ttggtatgcg tctgctggcg ttcgatccgt atccaagtgc agcggcgctg
     3901 gaactcggtg tggagtatgt cgatctgcca accctgttct ctgaatcaga cgttatctct
     3961 ctgcactgcc cgctgacacc ggcaaactac catctgttga acgaagccgc cttcgatcag
     4021 atgaaaaatg gcgtgatgat cgtcaatacc agtcgcggtg cattgattga ttctcaggca
     4081 gcaattgaag cgctgaaaaa tcagaaaatt ggttcgttgg gtatggacgt gtatgagaac
     4141 gaacgcgatc tgttctttga agataaatcc aacgacgtga tccaggatga cgtattccgt
     4201 cgcctgtctg cctgccacaa cgtgctgttt accgggcacc aggcattcct gacagcagaa
     4261 gctctgacca gtatttctca gactacgctg caaaacttaa gcaatctgga aaaaggcgaa
     4321 acctgcccga acgaactggt ttaatgttgc agctcccctt caatccaggg gagctgattc
     4381 agataatccc caatgacctt tcattctcta ttcttaaaat agtcctgagt cagaaactgt
     4441 aattgagaac cacaatgaag aaagtagccg cgtttgttgc gctaagcctg ctgatggcgg
     4501 gatgtgtaag taatgacaaa attgctgtta cgccagaaca gctacagcat catcgctttg
     4561 tgctggaaag cgtaaacggt aagcccgtga ccagcgataa aaatccgcca gaaatcagtt
     4621 ttggtgaaaa aatgatgatt tccggcagca tgtgtaaccg ctttagcggt gaaggcaaac
     4681 tgtctaatgg tgaactgaca gccaaagggc tggcaatgac ccgtatgatg tgcgctaacc
     4741 cgcagcttaa tgaactcgat aacaccatta gcgaaatgct gaaagaaggt gcacaagtgg
     4801 atctgaccgc gaaccagtta acgctggcga ccgcgaaaca gacattaact tataagctgg
     4861 cggatttaat gaattaatag ctgccacagc tcccggcggc aagtgactgt tcgctacagc
     4921 gtttgccgtt gggtaatgca cacatcccaa tcgccgtgcc atccagttga cgggcaacag
     4981 aaagcgaacc gccgatcatt gcacaatttg cttctccact actggacatc gacgctttta
     5041 aacctggcgc tacgtgcgcc gcagtcgcct gctgaacagg ttcactacta cacgccgaca
     5101 acaataaagc ggcacaccct acccaaaacg ctgctcgcat cttttcttcc tctgatcttc
     5161 aagccaaacg acaccgccat aaataatagg cagcacagag ggcgacatcg agagctgtcc
     5221 tgcgcgttgc cccgcatttt tactttttta tggctatttt tttgccctct gtttgatcaa
     5281 aacattcatt acgctgatgt gggggacaca aaagcgaaaa tgcagaagaa agccatttgc
     5341 taaaattgaa agattactac tgggcgcgca gcaatttcgt gcgcccctca tttgcgcaat
     5401 gtaagggtgt catatgatta ctattgacgg taatggcgcg gttgcttcgg tggcgtttcg
     5461 caccagtgaa gttatcgcca tctaccctat tacccccagt tccacgatgg cagaacaggc
     5521 tgatgcctgg gccggaaacg gcttgaagaa cgtttgggga gacacaccac gcgtggttga
     5581 aatgcagtcg gaagcgggtg ctatcgctac cgtgcatggc gctttgcaga cgggtgccct
     5641 ttcaacatcg tttacgtcat cgcagggttt gctgctgatg atcccgacac tgtacaaact
     5701 ggcaggcgaa ctcacaccgt ttgtcctgca tgtagcggca cgtaccgttg ccacacatgc
     5761 actctctatt tttggcgatc attccgacgt catggcggtg cgccagacgg gttgcgcgat
     5821 gttgtgtgcg gcaaacgtcc aggaagcgca agactttgct ctcatttcgc acatcgcgac
     5881 gctgaaaagc cgcgtgccat ttattcattt ctttgatggt ttccgcacgt cccacgaaat
     5941 caataaaatt gtcccgctgg ccgatgacac gattcttgat ctcatgccgc aggccgaaat
     6001 tgatgctcat cgcgcccggg cgctcaaccc ggaacatccg gtgatccgtg gtacgtccgc
     6061 caatcctgac tcttatttcc agtctcgcga agccaccaac ccctggtaca acgcagtcta
     6121 tgaccatgtt gaacaggcga tgaatgattt ctctgccgcg acaggtcgtc agtatcagcc
     6181 gtttgaatat tacgggcatc cgcaagctga acgggtgatt atcctgatgg gctcagccat
     6241 tggcacctgt gaagaagtgg ttgatgaatt gctaacccgt ggcgaaaaag tcggcgtgct
     6301 gaaagttcgc ctgtaccgcc ccttctcagc caaacacttg ctacaaactc taccgggatc
     6361 cgtacgcagc gtggcggtac tggacagaac caaagaaccc ggtgcccagg cagaaccgct
     6421 ctatctggat gtgatgaccg cactggcaga agcctttaat aatggcaagc gcgaaactct
     6481 gccccgtgtc attggtgggc gctatggtct ttcatccaaa gaatttggcc cggactgtgt
     6541 actggcggta tttgccgagc tcaacgcggc taaaccgaaa gcgcgcttta cggttggtat
     6601 ttacgatgat gtaaccaatc tgtccctgcc gttgccggaa aacacccagc caaactcggc
     6661 gaaacttgaa gccttgtttt atggccttgg tagtgatggc agcgtttccg cgaccaaaaa
     6721 caatatcaag attatcggta attccacgcc gtggtacgca cagggctatt ttgtttacga
     6781 ctccaaaaaa gcgggcggcc tgacggtttc tcaccttcgc gtgagcgaac agccgattcg
     6841 ttccgcttat ctcatttccc aggctgattt tgttggctgc caccagttgc agtttatcga
     6901 taaatatcag atggctgagc gtttaaaacc tggcggcatt ttcctgctca acacgccgta
     6961 cagcgcagat gaagtgtggt cgcgcttgcc gcaagaagtt caggccgtgt taaaccagaa
     7021 aaaagcgcgc ttctatgtga ttaacgcggc gaaaatcgcc cgcgaatgtg gcctggcggc
     7081 ccgtattaat accgtcatgc agatggcttt tttccatctg acgcaaattc tgcctggcga
     7141 tagcgccctc gcagaattgc agggtgcgat tgccaaaagt tacagtagca aaggccagga
     7201 tctggtggaa ggcaactggc aggctctggc gctggcgcgt gaatccgtag aagaagttcc
     7261 gttgcaaccg gtaaatccgc acagcgccaa tcgaccgcca gtggtttccg atgccgcccc
     7321 tgatttcgtg aaaaccgtaa ccgcagcgat gctcgcaggg cttggtgatg ccctccccgt
     7381 ttcggcgctg ccgccagacg gcacctggcc gatggggacc actcgctggg aaaaacgcaa
     7441 tatcgccgaa gagatcccca tctggaaaga ggaactctgt acccaatgta accactgcgt
     7501 cgccgcttgc ccacactcag ctattcgcgc aaaagtggta ccacccgaag cgatggaaaa
     7561 cgcccctgcc agcctgcatt cgctggatgt gaaatcgcgt gatatgcgcg ggcagaaata
     7621 tgtcttgcag gtggctccgg aagattgcac cggttgtaac ctgtgcgtcg aagtttgccc
     7681 ggcgaaagac cgtcagaatc cagagattaa agccatcaat atgatgtcgc gcctggaaca
     7741 tgtcgaagaa gagaaaatca attacgattt cttcctcaac ctgccagaaa tcgaccgtag
     7801 caaactggaa cgtattgata ttcgtacatc gcagctgatt acaccgctgt ttgaatattc
     7861 cggtgcttgc tccggttgtg gcgagacgcc gtatattaaa ttgctgactc agctctatgg
     7921 cgaccggatg ttgatcgcta acgccaccgg ctgttcttca atttatggcg gtaacctgcc
     7981 ctctacaccg tataccaccg atgccaacgg tcgtgggccg gcatgggcga actctctatt
     8041 tgaagataat gccgaatttg gccttggttt ccgcctgacg gtcgatcaac accgtgtccg
     8101 cgtgctgcgt ctgctggatc aatttgccga taaaatcccg gcggaattac tgacggcgtt
     8161 gaaatcagac gctacgccag aggttcgtcg agaacaggtc gcagctttac gccagcaact
     8221 caacgatgtt gccgaagcac atgaactgct acgtgatgca gatgcactgg tggaaaaatc
     8281 aatctggctg attggtggtg atggctgggc ttacgatatc ggctttggcg gtctggatca
     8341 tgtattgagt ttgacggaaa acgtcaacat tctggtgctg gatacgcaat gctattccaa
     8401 caccggtggt caggcgtcga aagcgacacc gctgggtgca gtaactaaat ttggcgagca
     8461 cggcaaacgt aaagcgcgta aagatcttgg cgtcagtatg atgatgtacg gtcatgttta
     8521 tgtggcgcag atttctctcg gcgcgcagct gaatcagacg gtgaaagcga ttcaggaagc
     8581 ggaagcgtat ccgggaccat cgctgatcat tgcttatagc ccgtgtgaag agcatggtta
     8641 cgatctggca ctcagccatg accagatgcg ccaactcaca gctaccggct tctggccgct
     8701 atatcgcttc gatcctcgtc gtgccgatga aggcaaactg ccgctggcgc tggattcacg
     8761 cccgccgtca gaagcactgg aagaaacgtt acttcacgag caacgtttcc gtcgtctgaa
     8821 ttcgcagcag ccagaagtgg cagaacagtt atggaaagat gctgcagctg atttgcaaaa
     8881 acgctatgac ttcctggcac aaatggccgg aaaagcggaa aaaagcaaca ccgattaaat
     8941 gctctggata aggattatcc aattctaaaa aaaagcccgg acgactgttc gggcttgtct
     9001 ttttatatgt tggaaaatca gtggcaatgc aatggcccaa cagaaaataa aatctgtgac
     9061 aataaaggca tataacccgc gcagaataac gtatagaaaa ttaaaagtat aatttttatt
     9121 ttgtatgtat tatcttcgat acattcacca gacttattat catttcagaa gaattatttg
     9181 attaaggttt tacttaaggc gtaacaaatg atatttatcg gctaactgaa cttctccttt
     9241 aggatgtttt cactccccgg aatggggata aaaggcaaat aaaataacta aaggatttat
     9301 tcaatgaaaa gcaaagtact ggcactttta attcctgccc tgctcgccgc aggtgctgca
     9361 catgcagccg aagtttataa taaagacggc aacaaattag atctgtatgg caaagttgat
     9421 ggcctgcatt atttttctga taattcagcg aaagatggcg atcagagcta tgcgcgtctg
     9481 ggttttaaag gcgaaaccca aattaacgat caactcactg gctacggtca atgggaatac
     9541 aatattcaag caaacaacac tgaatcttca aaaaaccagt catggacccg tctggcattt
     9601 gccgggctga aatttgcaga ttacggttct ttcgattacg gacgtaatta tggcgtaatg
     9661 tacgacatcg aaggctggac cgatatgctg cctgaatttg gcggtgactc ttataccaat
     9721 gcagacaact ttatgactgg tcgagccaat ggcgtcgcga cttatcgtaa tactgatttc
     9781 ttcggtctgg taaatggtct gaacttcgcg gtgcagtatc aaggtaacaa cgaaggagcc
     9841 agtaatggtc aggaaggcac caacaacgga cgtgatgttc gccatgaaaa cggtgacggc
     9901 tggggttttt ccacaacata tgatttaggc atgggcttta gcgctggtgc ggcatacacc
     9961 tcttctgacc gcaccaatga ccaggttaac catactgcgg cgggtggtga taaagcagac
    10021 gcgtggactg ctgggctaaa atacgatgct aacaatattt acctggcaac catgtattca
    10081 gaaacgcgta atatgacccc gtttggcgac agcgattatg ctgtggcaaa caaaacccag
    10141 aattttgaag tcactgccca gtaccagttt gattttggtc tgcgtccggc agtctctttc
    10201 ctgatgtcta aaggccgtga cctgcacgct gccggtggtg cagacaaccc ggcaggtgtt
    10261 gatgataaag atctggttaa atacgccgat gttggcgcga cttactattt caataaaaac
    10321 atgtccacct acgttgacta taaaatcaac ctgttggatg aagatgacag cttctacgct
    10381 gccaatggca tctctaccga tgatattgtc gctttaggtc tggtttatca gttctaaatc
    10441 ctcctgcccg ctgttatggc gggctttttc tgcttattct tcctcctttg atctaaatta
    10501 aaaatgtgaa ctccatcatt acacaaaaag tgtcatctgg cgttacactt tatgcggata
    10561 ctaaaacagg aggttttatg aacagaacga ttcttgtccc tatcgatatt tccgattcag
    10621 aattaactca acgcgtgatt agccacgttg aggcagaggc aaagattgac gatgcagagg
    10681 ttcatttcct gacggtaata ccttcactgc cctactatgc ctctctgggt ttagcgtatt
    10741 ccgcagaatt accggcaatg gatgacctga aagcggaagc caaatcgcaa ctggaagaga
    10801 tcattaaaaa atttaaactg ccaaccgaca gagtgcatgt ccatgttgag gaaggctcgc
    10861 ccaaagaccg cattctggaa ttggcgaaga agatccccgc tcatatgatc atcattgctt
    10921 cccatcgacc ggatatcacc acttatctgc tcggttccaa cgccgcagct gtagtgcgtc
    10981 acgcagagtg ctccgtgctg gttgtgcgct gacactaacg cccgcacatt gctgcgggct
    11041 ttttgattca tttcgcaaat gtgctgacat tttcccctct aatccgtacc atacacgcca
    11101 ctgtttttat atcagatttc ttatgctggg cattccggca tgacgcatac tcttctgatg
    11161 ccatataacg aattgagtcg cttttaaatg tcgcaaaatc aagaaattag taagaaagaa
    11221 caatacaacc tgaacaagta agggcaaaaa tcacaactat ctgactcata agtattttat
    11281 ttatttttca atatgttaaa cattatcagc gacaacaata agctacgata acccactctt
    11341 tttttgcccc atttttgcca tcaacaattt tcctcataaa gtcatttcaa agcctaatag
    11401 ctgctctact tttttcctca atatcatgtc gccagcagga aacaacctaa tccctgtttt
    11461 aggcctcaaa gctgtgttaa tgataattat tttcattttg tataggttat gctataaaac
    11521 atagccgttg ctatgtttgt tctaattttg ctcactatag gtactaaatt atgcactcaa
    11581 taaaaaaagt aaccatgctc ttggggtgcc tcgccctcac ctgctcgatc gcatttcagg
    11641 caagtgcaac tgaaaaattc aaggtcatta caacatttac catcatcgcg gatatggcca
    11701 aaaacgtggc tggagatgct gcagaagtct catccataac caagcctggt gcagaaattc
    11761 atgagtatca gcctacccct ggcgatatta aacgtgcaca gggggcacaa ctgattctcg
    11821 ccaatggtat gaatctggaa ttgtggttcc aacgctttta ccagcatctc aatggggttc
    11881 cagaagtaat tgtctcttcg ggtgtgacgc cagtagggat caccgaagga ccctatgagg
    11941 gcaaacctaa tccccatgca tggatgtcgc cagataatgc tctgatttac gtcgataata
    12001 ttcgtgatgc gttgataaaa tacgatccgg caaatgcaca aacctaccaa cgtaatgccg
    12061 atacttataa agccaagatt acccaaaccc ttgcccccct gcgtaagcag attacggaac
    12121 tccctgagaa tcagcgatgg atggtcacca gtgaaggggc tttttcttat ctcgcacgcg
    12181 atttggggct aaaagagctt tatctgtggc cgattaatgc cgatcaacaa ggaacaccgc
    12241 agcaggtacg taaggttgtt gatatagtta agaaaaatca tatcccggca gtctttagcg
    12301 agagtacgat ttccgataaa ccagcgcgtc aggttgcgcg tgaaaccggc gcacactacg
    12361 gcggtgtgct ctatgtcgat tccctgagca cagaaaacgg cccggtacct acgtatatcg
    12421 accttcttaa ggttactacc agtacgctgg tacagggaat taaagccggg aaaagggaga
    12481 aataatgatg caatctgcag gcattgtcgt taatgatgtt actgtcacct ggcgtaacgg
    12541 gcacacagca ctgcgcgatg catccttcac agtaccaagt ggatctattg ccgctctggt
    12601 tggggtaaac ggttccggaa aatcgacgtt gtttaaagcg attatgggat ttgtgcgtct
    12661 gaccagcgga aagatatctg ttctgggtat tcccacgcga caggcgctac agaaaaacct
    12721 ggttgcctac gtccctcagt cagaagaggt tgactggtca tttcctgtac tggtggaaga
    12781 tgtggtgatg atgggccgct atgggtatat gggtatgttg cgtatagcca aaaagaggga
    12841 tcgtcagatt gtcaccgatg cgctggaacg tgtcgatatg gtggattttc gccatcgtca
    12901 aatcggcgag ctttccggtg gacaaaagaa acgcgtcttt ctggcgagag cgattgcaca
    12961 gcagggtgat gtgatcctgc tggatgaacc ttttaccggc gttgatgtga aaaccgaagc
    13021 gaaaatcatc agcctgttga gggagttacg cgcggaaggc aaaacgatgc ttgtctcaac
    13081 gcacaatctc ggctcagtca cgacattttg cgattatacg gtcatggtca aaggcaccgt
    13141 cctggcaagc gggccgacag acaccacttt tacagccgag aacctggagc tggcttttag
    13201 cggcgtactg cgccatgtca cacttaacgg ctcggaagaa agcattatta ctgacgatga
    13261 acgccctttt gtggcacatc gaccgtcagc ggtgcagagg gaagcaagat gaacgtgcta
    13321 ctggaaccct tcagctatga atatatgctc aatgcgatgt gggtctcggc aatggtcggc
    13381 ggtctctgcg ctttcctgtc atgctatctg atgctaaaag gctggtcgct gattggcgat
    13441 gcgctgtcgc actctattgt cccgggtgtt gcgggggcat atatgctggg gcttccgttt
    13501 tctctgggag ccttcttttc cggcggactg gcagcgggca gcatgctgtt tcttaaccaa
    13561 cgcacccgct taaaagaaga tgccatcatc ggcctgatct tctcgtcctt ttttggcctg
    13621 gggctgttta tggtgtcatt gaacccgaca tccgtgaaca ttcagaccat cgtcctgggc
    13681 aatattctgg caatcgatcc ggcggatatc ctgcaactca ctatcattgg cattctctcg
    13741 atcatcgtgc tttttttcaa atggaaagat cttatggtga cctttttcga cgagaatcac
    13801 gcccgtgcta ttggattaca tccgggcaga ttaaaaatcc tcttcttcac cctgctgtcg
    13861 gtttcgaccg ttgcggcgct gcaaacggtt ggtgcctttc tggtgatctg tctggtggtg
    13921 actcccggtg caacagcatg gttgttaacc gatcgttttc cccgcctgct cataattgct
    13981 gtaactattg gcagtgtaac cagtttcctc ggcgcgtggg tcagttactt tctggacggc
    14041 gcgacaggtg gaatcatcgt ggtggcgcag acactgctgt ttcttttagc atttgtattt
    14101 gcgcccaccc atggactttt ggcaaatcgt cggcgtgcgc ataaagcgct ggaggatcgc
    14161 tcatgatggc attattactg gagccactgc agttcacctt tatgagtcac gcactgctga
    14221 tttcactggt agtttccata ccctgtgcgc tgctgtcggt ctttctggtc ctgaaaggtt
    14281 gggcactgat gggtgacgcg atgagtcatg cggtttttcc tggcatcgtg cttacctgga
    14341 ttctggggtt acctctggct acaggggcat tcgtcgccgg agtattctgc gcggtggcga
    14401 cgggatacct gaaagacaac agccgaatca agcaagatac agttatgggg attgtttttt
    14461 ctggcatgtt tgccgcaggt ctgatcttgt atattgcaat caaaccagac gtacatcttg
    14521 accatattct tttcggcgac atgctcggga taaccatcgg cgatataatc cagacggtga
    14581 ttattgccgg gctggttaca cttgttatta gcgtaaaatg gcgggatttt ttgctgttca
    14641 gctttgatta tcaacaggcg caggtaagcg gtttgcatac gagatggctg cattatgggc
    14701 tgctgtgcat ggtttccctg accattgtgg cgacgctgaa agcagtaggt atcattctct
    14761 ctatttctct gctcattgcg cctggcgcca ttgcagtact cctgacacaa cgctttcata
    14821 ttgcgctcct actggcgact ggcatatcgg taatagtgtc aatgactggt gtctggctgt
    14881 ccttttttat cgacagcgca ccagctccga cgattgtcgt cttattcgcg gtcgtgttca
    14941 tcatgacgtt taccgttacc agcatcaacg cacgcacaaa gggaaacgcc catacacagg
    15001 atctgttatc acccaattag cacaatatcc gaggacagca tcgtccgcca gcctacagaa
    15061 gttcggaaag ccatggaagg tcgttgggag gaggtacgaa ttctatcatg caaaaaatac
    15121 gtaaaatcga taacgcctag aaatcattca atactcgcac tatcgaaagt ttaccagcca
    15181 accgcggcac gttcctgcat acgacgtgcc gcagcttctg ttattccacg ataaaattcc
    15241 taaccgaatt caaatccttc agactattca gctcttcctt cagctcccgc tgacggcgat
    15301 aaatcgtaag cgccccgaga agtacgtagc gtaaggatta ttttacagac gagaagttcc
    15361 agggcagcag ttcatgcact tggttcgacg gccagtcatt gagcttctcg atcacttcgc
    15421 gcaaccagtc ctccggctcc acttcgttct gtttgcaggt gaccagcaga ctgtagatga
    15481 tcgccgcact ttctcctccc ttgtctgagc cgaaaaagag ataatttttt cttccaaccg
    15541 ccaccgatcg taacgcgttt tcaccgatgt tgttgtctat ttccacccag ccgtcacgac
    15601 agaactcgtt cagcgcattc cagtgattca ggatatagtc gaacgccttc gccatctccg
    15661 catgtttcga cagcgttttc ctctgcaact gtatccagtc gtacaatgac tgcatcaact
    15721 ggacgcttct ggcttttctg actgcaagcc gttcctctgc cggactgcca cgtatctccg
    15781 cttctatgtc gtataactct gctatccgtc tgagcgcttc ctgagtcatt tctgtcggac
    15841 ggcgcacatc ctcgtcatgg atttttcggc gggcgtgggc caggcacccg gcttccttca
    15901 cccggcccgt ttcgtacagt acgttataac ctgcataggc atcagcctgc agtacgccct
    15961 gatactttgc caggtggagc tgcggatgtt ctcctttgcg atctgccgaa tacgcgaacc
    16021 agacggctgc cggcagggat gaacccgcat tacgatcatc cctgacgtat acccacagac
    16081 gacccgtttt cgtctttccg ttccccgggg cca
//
LOCUS       DADLFA010000094        15168 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000094 DADLFA010000000
VERSION     DADLFA010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15168
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.094"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..450
                     /locus_tag="JQH65_002460"
     CDS             <1..450
                     /locus_tag="JQH65_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY6139846.1"
                     /translation="GPPKTNAGIRTVTLLQPALEALKEQYKLTGHHRKSEITFYHREY
                     GRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGARWNAAVKRAGIRRRNPYHTRHTFACW
                     LLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARLS"
     gene            502..1437
                     /gene="ttcA"
                     /locus_tag="JQH65_002461"
     CDS             502..1437
                     /gene="ttcA"
                     /locus_tag="JQH65_002461"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HAY6139847.1"
                     /translation="MQENQQITKKEQYSLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPVYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(1566..2939)
                     /gene="dbpA"
                     /locus_tag="JQH65_002462"
     CDS             complement(1566..2939)
                     /gene="dbpA"
                     /locus_tag="JQH65_002462"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HAY6139848.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAVISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(3385..4368)
                     /gene="zntB"
                     /locus_tag="JQH65_002463"
     CDS             complement(3385..4368)
                     /gene="zntB"
                     /locus_tag="JQH65_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HAY6139849.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            4623..5855
                     /gene="dgcM"
                     /locus_tag="JQH65_002464"
     CDS             4623..5855
                     /gene="dgcM"
                     /locus_tag="JQH65_002464"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HAY6139850.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(5876..6439)
                     /gene="smrA"
                     /locus_tag="JQH65_002465"
     CDS             complement(5876..6439)
                     /gene="smrA"
                     /locus_tag="JQH65_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HAY6139851.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIHQALADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(6769..7677)
                     /locus_tag="JQH65_002466"
     CDS             complement(6769..7677)
                     /locus_tag="JQH65_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY6139852.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTPSLITQFRRECGYLQS"
     gene            7853..9153
                     /locus_tag="JQH65_002467"
                     /pseudo
     CDS             7853..9153
                     /locus_tag="JQH65_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
     gene            9153..10598
                     /locus_tag="JQH65_002468"
     CDS             9153..10598
                     /locus_tag="JQH65_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAY6139853.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGYIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSVTP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANFPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFTKQIQ
                     ATLPPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTLNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTLSSLK"
     gene            10635..12161
                     /gene="abgT"
                     /locus_tag="JQH65_002469"
                     /pseudo
     CDS             10635..12161
                     /gene="abgT"
                     /locus_tag="JQH65_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
     gene            12172..12687
                     /gene="ogt"
                     /locus_tag="JQH65_002470"
     CDS             12172..12687
                     /gene="ogt"
                     /locus_tag="JQH65_002470"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAY6139854.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLSDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            12882..13634
                     /locus_tag="JQH65_002471"
     CDS             12882..13634
                     /locus_tag="JQH65_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FNR family transcription factor"
                     /protein_id="HAY6139855.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            13786..14736
                     /gene="uspE"
                     /locus_tag="JQH65_002472"
     CDS             13786..14736
                     /gene="uspE"
                     /locus_tag="JQH65_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HAY6139856.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFEPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(14786..15044)
                     /locus_tag="JQH65_002473"
                     /pseudo
     CDS             complement(14786..15044)
                     /locus_tag="JQH65_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
BASE COUNT         3857 a         3811 c         3734 g         3766 t
ORIGIN      
        1 ggtcccccaa aaacaaatgc cgggatccgg acggtaacat tactgcagcc ggctcttgaa
       61 gcactgaagg agcaatacaa actgaccggg catcatcgca aaagcgaaat cactttttat
      121 catcgggagt acggcagaac tgaaaagcaa aaactgcatt ttgttttcat gcccagggtg
      181 tgtaacgaaa aacagaaacc ttattactcg gtaagcagtt tgggtgcaag atggaatgca
      241 gcagtaaaac gtgctggtat tcgccgccgt aatccgtacc atacgcgaca tacttttgcc
      301 tgctggctgt tgacggcagg agcgaacccg gcatttatag ccagccagat ggggcatgaa
      361 actgcgcaaa tggtgtatga aatttacggt atgtggattg atgacatgaa cgacgaacag
      421 atagccatgt tgaatgcgcg gttatcgtag ttgcaaagtt tgcccccaat ttgccccatt
      481 tagtaccaga gaactgaaat aatgcaagaa aatcaacaaa ttacaaagaa agaacaatac
      541 agcctgaaca aattacaaaa acgtctacgt cgtaacgtgg gcgaagccat tgctgacttc
      601 aatatgattg aagaaggcga tcgcatcatg gtttgcctct cagggggtaa agacagctat
      661 accatgctgg agattctgcg caatttgcag caaagcgcgc caatcaattt ttcgctggtg
      721 gctgttaacc tcgatcaaaa gcaaccgggc ttcccagaac acgttctgcc cgtgtatctt
      781 gaaaagctgg gcgttgagta caagattgtc gaagagaaca cttacggtat cgtgaaagag
      841 aagattccag agggcaaaac cacttgctca ctgtgttctc gccttcgtcg cggtatcctt
      901 tatcgtaccg caacggaact gggggcgacg aagatcgcgc tgggtcacca tcgtgacgat
      961 atcctgcaga cgttgttctt aaatatgttc tacggcggca agatgaaagg tatgcctccg
     1021 aaactgatga gcgatgatgg caaacatatc gttattcgtc cgctggccta ctgccgcgag
     1081 aaagatattc agcgatttgc cgatgcaaaa gcgttcccga ttattccgtg caacctgtgc
     1141 ggttcacagc ctaacctgca acgtcaggtg attgctgaca tgttgcgtga ctgggataaa
     1201 cgttatccag ggcgtatcga gacgatgttc agcgcgatgc agaatgtggt gccgtcgcat
     1261 ctgtgcgata ccaacctgtt cgatttcaaa ggcatcaccc acggttctga agtggttaac
     1321 gggggtgatc tggcgtttga tcgcgaagag atcccactac aaccggcggg ctggcagcca
     1381 gaagaagatg aaaatcagtt ggatgagtta cggctgaatg tggttgaagt gaaataacca
     1441 ggatagcgcc cgatgcgcaa gcgtatcggg ctactcttat ggaggccgga taagacgcgg
     1501 ccaacgtcgc atccggcaat gccgaagaag atgtttactc ttgcacccgg caattcaaca
     1561 tttcattatt ttaataaccg cacccggcac gtttttcctt taatcttccc gccctgtaac
     1621 tgtttccatg ctttatgagc aacagcctga cggaccgcga catagacatg cgccggatgc
     1681 acggcgattt tgccaatatc tgcgccatca agcccgatat ctcctgtcag tgcacctaat
     1741 acatcacccg ggcgcatttt ggcttttttc ccgccatcga tacacaacgt tgccatttct
     1801 gcttccagcg gcacaatgga actattagct ggcggcgttt gccagttaag ttttatctgc
     1861 aacatgtcag aaatgatatt ggcccgctgt gcttcttccg gagcacagaa actgatcgcc
     1921 agaccgctat ttcctgcacg agctgtacgg ccgatgcgat gtacatgaac ttcagggtcc
     1981 cacgccagct caaagttcac caccagctca agcgatttaa tatccagacc acgcgcagca
     2041 acatcagtcg caaccagtac acgggcgcta ccgttagcaa aacgtaccag ggtctgatcg
     2101 cgatcgcgtt gctccaaatc gccgtgtaat gacaatgcac tttgccctac ttcattcagc
     2161 gcgtcgcaga cagcctggca atcttttttg gtattgcaaa acaccacgca agaggatggc
     2221 tgatgcaagc ttaataaccg ttgcaacaga ggaattttgc ctttgctgga tgtctcataa
     2281 aattgttgtt caatgggtgg caaagcatct gttgagtcaa tttcaatcgc caaaggatcg
     2341 cgttgcactc gtccgctgat tacggcgatg gcttccggcc aggttgccga aaacagaagc
     2401 gtctgtcgag atgcaggcgc aaaacggatg acatcatcaa tggcatcgct aaatcccata
     2461 tccagcatgc ggtcggcctc atccatcacc aaagtattca gcgcatccag tgataccgtg
     2521 cctttttgca agtgatccag caaacgaccc ggcgttgcca cgataatatg cggcgcatgt
     2581 tgcaacgaat cacgctgcat accgaacggt tgaccaccgc acaacgtcaa aattttggta
     2641 tttggcagaa aacgcgccag ccgacgcaat tcacctgcca cctgatccgc cagttcacgc
     2701 gtaggacaca gcactaaagc ctgggtttga aatagcgacg catcaatttg ctgtaacaag
     2761 ccgaggccaa aagccgccgt tttgccgctg ccggttttcg cctgcacgcg aacatctttt
     2821 ccggcaagga tcgccggaag cgcggcggcc tgcaccggcg tcatagttaa ataacccaac
     2881 tcattaaggt tcgtgagttg ggcgggaggc aaaacattca gggtagaaaa agcggtcaca
     2941 atctattctc gtggtcatcg acgcaaagtt agcaggcgcg tatcctcgca gatctacgcg
     3001 ggtggtgggt caggcatggg ttgcgggcga gggatcggat cgggcactgg aacaggatcg
     3061 ccaggaatcg gttcagggac aggaatttgc aaataaataa gtgtcgtcat atttccctcc
     3121 ggtcattggg tggactctta aagggtagac gctgataaat aacaggcaaa aaaaagccga
     3181 ctcatcaaag tcggcgtcgt acgaatcaat tgtgctatgc agtaattcaa aaaaggaagt
     3241 aagacaatat ggagcgcaac gcccatcgct tgacgttgca ttcacctgca agggagatat
     3301 tgccctgaat gggtagagag tttattgact tcgctcaaac tttgcggcgt ttttgtatac
     3361 agacagccgg aaaaattgct tttgttacaa ccatttacta cgatgcaacc ataaagcaac
     3421 accaccaata agaacaacta acagaataca aaaaattgaa aatccgaatt gccacccgcc
     3481 gccagggatc ccaccaaggt tgacgccaaa taaccctgtc agaaaggtac tgggtaaaaa
     3541 gaccattgcc atcaatgaca ttgtataggt acgacgagct aaattttcct gcatcacctg
     3601 agcgatttca tccgccatca cgccagtccg tgctatacag gcgtcgattt cgtcaagacc
     3661 gcgcccaagg cgatcggcaa tatcctgcat ccgacggcgt tggtcatcgc tcatccacgg
     3721 caaacgttca ctggcaagac gagcataaac atcacgttgc ggtgccatat agcgacgcat
     3781 cacgattaat tgtttgcgca gcagagccag gaatccacgc ggtggaattt gctgatcaag
     3841 gagattatct tcaaggtcga taattttatc gtgcagctgc tcgataaatt cgctggaatg
     3901 atcggtcaac gcatcgcaca catccaccag ccaccctccg caatcggtcg gacccgtgcc
     3961 ctcttccaga tcgctcacca catcgtccag cgccagcact ttgcgttgtc gggtcgaaac
     4021 aattaaccgc ccgtccatat atacacgcat ggcgaccagt tgatcggggc gttcatcggt
     4081 gctgccgttt atacagcgca atgtaatcag cgtgccttca ccgagacggc tgactcgggg
     4141 ccgtgtgctc tcgcctgcca gcgcatcacg tacgttattg ggaagcagcg gtgttgtcgc
     4201 cagccattgg gcgctatcat ggtgtacata attaaggtgg agccagcagg gatgcgcttc
     4261 atcaatcaca tctgtatttt ccagcggttt aacgccgcct ctaccatcca gcatccaggc
     4321 aaatactgca tccgggacat taacgtccga tcccttaatc gcttccacag tgcctccatc
     4381 atcaacgcat tattttgtag tctagccttc tggccctgtt acgcaacatc tcatcacccc
     4441 attaccctga aatgattaat aaaattctgt ctaaattgaa tacaaaaagc aaaatgcttt
     4501 tccgtataca aaccgtgtga agtgttaaat agcgtctatc attatcagaa ttatctgatc
     4561 atatgacgtg gcttttttgc gatcggatag caacaaaaat tgataaaaat aacgggatct
     4621 caatgattac gcacaacttc aataccctgg acttactcac cagtcctgtc tggattgttt
     4681 cgccctttga ggaacagtta atttatgcca atagcgcggc gcgactgttg atgcaagacc
     4741 tcacgtttag tcagctacga accggaccct attccgtctc ctcacaaaaa gaactgccga
     4801 aatacctctc cgatctgcaa aaccaacacg atattatcga aatcctcact gttcagcgta
     4861 aagaagagga aacagcatta agctgtcggc ttgttttgcg agagctgaca gaaacagaac
     4921 cggtgattat tttcgaaggt atcgaagcgc cggcaacgct gggtttaaaa gccagtcgct
     4981 cggcaaatta tcagcgcaaa aaacaaggtt tttatgcgcg cttttttctg actaactctg
     5041 caccaatgtt gttgattgac ccctcacgag atggccaaat cgtcgatgct aacctcgccg
     5101 cgctcaattt ctacggttat aaccatgaaa cgatgtgcca gaaacatacc tgggaaatta
     5161 atatgctcgg gcgtcgcgtc atgcctatca tgcatgaaat ttcgcattta cccggcggtc
     5221 ataaaccttt gaattttgtt cataaactgg cggatggatc tactcgtcat gtgcagacct
     5281 atgccgggcc gattgaaatt tatggcgaca agctcatgtt atgtattgtg catgatatta
     5341 ctgagcaaaa acggctggag gagcagctgg aacatgctgc tcaccatgac gcgatgaccg
     5401 gattactgaa tcggcgacag ttttatcaca ttacggaacc aggccaaatg cagcacctcg
     5461 ccatcgccca ggattacagc ttgttgctca tcgacaccga tcgttttaaa cacattaacg
     5521 atctctatgg gcattctaaa ggtgatgagg tgttatgcgc cctcgcccgc accctcgaaa
     5581 gttgcgctcg caaaggcgat ttggtgtttc gttggggagg cgaagagttt gtcttattgc
     5641 taccaagaac cccactggat accgcgcttt cgctggctga aactatccgt gtaagcgtgg
     5701 ccaaagtgag tatttcgggc ttaccacgtt ttaccgtcag cattggtgtg gcgcatcacg
     5761 aaggaaatga aagcatcgat gaactgttta aacgcgttga tgatgctttg tatcgggcga
     5821 aaaatgatgg acgcaaccgc gtgctggcgg cataagccgc ggatgcgtct cgagatcaac
     5881 ggctgcgctt agcgtggcgc tcccagtttt cttgcttcgc ctgcgccgtt ttacgtagtg
     5941 cgacgtaaca cgccccgctg ccgccatgat gcggtagcgc ggtgcaatat gcctgaacat
     6001 catcaaattc ggtcagccag cgcgccacat agctgcggac aatattggca tgcgatttat
     6061 cttcccgccc tttaccatga ataatcagca cattacgcaa accatccgcc agggcttggt
     6121 gaatgaaact gaacaccatt ttgcggcact cttccaccgg ctggcgcaaa agattcaggc
     6181 tcgcctgttg cggatattta ccactgcgca gcttatccag caccccatgt tgcaaccctt
     6241 cccgccgaaa ctccagcggc tgacttagcg ggatgatgtc gagaaatccg gtggtgagga
     6301 aattatcaag ctgcagcgtg tcgatacgct gcggcgcacg ttggttacgc gttggatgcc
     6361 agtggacatc tgtagcacgt ttcagcggct ggacatcttc catggcgtca agaaacagcg
     6421 atttgtcgtc aaggttcatg ttacatcctc ccgcaattaa gagcgcgata tgataaccag
     6481 accgggtcgg tccaacaacg tattaccaaa aattccagta ataagttcca aatattgccg
     6541 atattttaag caaaatgctt atgcatgatt attcattcac gatattaata atgtaactta
     6601 tattttcgtg aaatctgtca ctgaagaaaa ttggcaacta aaggttaaaa ccgttataac
     6661 acagtcaccg gcgcagagga gacaatgccg gacttaagac gcggatgcac tgctgtgtgt
     6721 actgtagagt ctggcggatg tcgacagact ctattttttt atgcagtttt aactttgcag
     6781 atagccgcat tctcgccgga attgcgtgat taatgacggg gtcagtggtg tctggcgact
     6841 atcacgccgc tgaatcaaat aataggccgc tttcggtaaa atttcgctaa ccggcaacat
     6901 caccagcccc tgtccgtgca agggatcgca gcccatttct tcaggcagta tgctgagaaa
     6961 atcgcttttt gccaccagac tgatacaggc tgagaacgtc tcgcagacta caccgacctg
     7021 tggcgtttgc gcctgatcgt caagcaattc actcaactgt ttgtagtaac tgccgtgtgg
     7081 cgtcggcatt gtccagctgt aatccagtaa ctgtttgatc gaacgggcac caatggcggg
     7141 gtgtcccggg cggcaaaaga tcgcgaattg cttttccagt aatttctcaa aagtaaattc
     7201 gtggtcgtac ggtccctgat aataggtatt gatggtgaaa tccaattctc cctgacgcaa
     7261 ttcattaatc atcgacacca gttgcccttc cataatgcgt acttttacct gcggatgctg
     7321 ctgatgaaaa cgagatatga cagctggcat cagactgcgg gaaatactgg cccccatgcc
     7381 gatattaatc tgccctgcca gttgcccttg tcgttggcga atatcctctt gggctgcgcg
     7441 cagctcttca agaataagac tggcgtgctg ataaaaactt tcaccggcat cagttaacgt
     7501 cacgccttta ctacggcgaa aaaagagttg cgccgctaat ccttcttcca actcctgaat
     7561 agatttactc agtgccggtt gcgacatatt caacattcgg ctcgctccgc gaatgctgcc
     7621 ctgacgagcc acttcaacaa aagcacgaat ttgatgaatt tttacctgaa aagccatgac
     7681 gccaccgata accgttattt atcagaccaa agaaactggc atctacttta atgcagatga
     7741 ttgtgtcagg gtaatttatg aacggttaaa actgtgaaaa atcagttagt gataagtaaa
     7801 aactatcgct acgtgaaccg ggtcacactt tttactgatg acgggaaagg ttatggagtc
     7861 tttgaatcaa tttgttaatt cgcttgcccc aaaattatcg cactggcgac gtgattttca
     7921 tcactatgca gagtctggct gggtggaatt ccgcactgcc acccttgttg cggaagaatt
     7981 gcagcagctc ggctattcac tggcgctggg ccgcgaagta gttaatgaaa gtagccggat
     8041 gggactacct gatgaactca ctctacaacg cgaattcgag cgcgctcgtc aacagggtgc
     8101 gctagcacaa tggattgcgg tttttgaagg tggtttcact ggtatcgtcg ccaccctgga
     8161 taccggtcgc cccggtccgg tgatggcttt ccgtgtcgat atggacgcgc tggatctcag
     8221 tgaagagcag gatgtcagcc atcgccccta ccgcgacggt tttgcgtcat gtaacgccgg
     8281 aatgatgcat gcctgtggtc atgatggaca taccgccatt gggcttgggc tagcgcatac
     8341 ccttaaacaa ttcgagtccg gactacatgg cgtcatcaaa ctgatttttc agcctgcaga
     8401 ggaaggtacg cgtggcgcgc gggcgatggt cgatgcaggt gtcgtagatg atgttgatta
     8461 ttttactgcc gtgcacattg gcactggcgt acctgcgggc accgtggtgt gcggcagtga
     8521 taattttatg gcaaccacca aatttgacgc gcacttcacc ggtaccgccg ctcacgcagg
     8581 cgcaaaacca gaagacggtc acaatgcctt gttggcggca gcacaagcca ctcttgcact
     8641 gcatgcaatc gccccggagc ttccagagta aacgtgggcg ttatgcaggc aggaagcggt
     8701 cgtaacgttg ttcctgcctc ggcgttgctg aaagtggaaa cacgcggggc cagcgacgtc
     8761 attaatcaat atgtttttga ccgtgcacaa caagcgattc agggcgcagc aaccatgtat
     8821 ggtgtcggcg ttgaaactcg tctgatgggt gcagctaccg ccagttctcc ttcgccgcaa
     8881 tgggtcgcat ggttgcaaag tcaggcggct caggtcgcgg ggggcaatca ggccattgaa
     8941 cgtgttgaag cgcctgcggg ttccgaagat gccacattaa tgatggcccg cgtgcagcaa
     9001 catcaagggc aagcctccta cgtggtgttt ggcacacagc tggcggcagg tcatcacaac
     9061 gaaaaattcg attttgacga gcaggttctc gctattgccg tcgaaacgct ggcgcgcacc
     9121 gcgctcaatt ttccctggac gcgaggtatc tgatgcagga aatctatcgt tttatcgacg
     9181 atgcgattga agccgatcgc caacgttata ccgatattgc cgatcaaatc tgggatcatc
     9241 cagaaacacg ttttgaagag ttctggtcag cggagcatct ggcttcggcg ctggaatctg
     9301 caggcttcac cgttacccgc aacgtaggct atatcccaaa tgcctttatt gcttcgtttg
     9361 gtcaaggcaa accggttatc gccctgctgg gagaatatga cgccctggca ggtttaagtc
     9421 agcaagcagg ttgcgcgcaa cctacatccg tgacgcccgg tgaaaatggt cacggttgcg
     9481 gacacaattt gctgggaacc gccgcctttg ccgctgcaat agccgtcaag aaatggctgg
     9541 aacaatatgg gcaaggcggc acggtgcgct tttatggttg tcctggcgaa gaaggcggct
     9601 cgggtaaaac gttcatggtt cgcgaggggg tatttgatga tgtggatgcg gcactcactt
     9661 ggcacccgga agcctttgcc ggtatgttca atacccgcac gctggcaaac attcaggcat
     9721 catggcgctt taaagggatc gcagcacatg ccgcgaattt ccctcatttg ggacgcagcg
     9781 cccttgatgc cgtaacgttg atgaccactg gcaccaactt cctcaacgaa catattattg
     9841 aaaaagcgcg cgtacactat gccatcacaa atagcggcgg gatctcgccc aacgtggtcc
     9901 aggcgcaggc agaagtgctt tatcttatcc gcgcccccga aatgaccgac gtgcagcata
     9961 tttatgatcg ggtcgccaaa atcgccgaag gtgcggcatt gatgaccgaa accacggttg
    10021 aatgccgctt cgacaaagcc tgttccagtt atctcccgaa tcgcacctta gaaaatgcca
    10081 tgtaccatgc cctatcccat tttggtaccc cggaatggaa ctccgaagaa ctggctttta
    10141 cgaaacaaat tcaggctacg ctccccccca acgatcggca aaacagtctg aataatatcg
    10201 ccgcaaccgg tggcgaaaac ggcaaggctt ttgcactacg tcatcgtgaa acggtactgg
    10261 cgaatgaggt cgctccatat gccgccaccg ataacgtgct tgcggcatcg actgatgtcg
    10321 gcgacgtcag ttggaaactg cctgttgccc agtgtttcag cccctgtttt gccgtcggta
    10381 caccgctaca tacgtggcaa ctggttagcc aggggcgaac atctattgct cataaaggaa
    10441 tgctgctggc ggcgaaaact atggcagcaa ccacactcaa tctcttcatt gattcagggc
    10501 tattgcaaga atgccaacaa gagcatcagc aagttacgga cacgcaaccg tatcactgcc
    10561 ctatcccaaa aaacgtgaca ctgtcatctt taaaataaca acaacaacgc aaacacaaca
    10621 accgaggaat gcccatgagt atgtcatcca taccgtcgtc ctcctaatcc gggaagctct
    10681 atggctgggt cgaaagaatt ggtaacaagg ttccccatcc ttttttgctc tttatctatt
    10741 tgattatcgt actcatggtg acgacggcaa ttttgtcggc ctttggcgtc agtgcgaaaa
    10801 acccgaccga tggaacgccg gtggtggtga aaaacctgct cagtgtggaa ggattacact
    10861 ggtttttacc caatgttatt aaaaacttta gcggttttgc tccacttggt gcgatcctgg
    10921 cgctggtttt aggtgccggt ctggcggagc gcgtcggctt actgccagcg ctaatggtta
    10981 aaatggcatc gcatgttaat gtccgctacg ccagttatat ggtgctgttt attgcttttt
    11041 tcagccacat ttcttccgat gcggcgttag tgatcatgcc accgatgggt gcgctgattt
    11101 ttctggcggt gggcaggcat ccagttgcag gtttactggc tgccattgca ggcgtaggtt
    11161 gcggctttac ggctaattta ctgattgtca caaccgacgt gttgctgtcg gggatccgca
    11221 cggaagcggc ggctgcgttc aatccgcaaa tgcacgtcag tgtaattgat aactggtatt
    11281 ttatggccag ctccgtagtc gtactgacga ttgttggcgg cctgataacc gacaaaatca
    11341 tcgagccacg gttaggtcaa tggcagggaa acagcgatga gaaactgcag acattgaccg
    11401 aaagtcagcg ttttggttta cgcatagcag gtgtcgtatc gctacttttt attgctgcga
    11461 tcgcgctgat ggtgatcccg gaaaacggga tattgcgcga tccgattaat cacaccgtga
    11521 tgccatcacc ctttattaaa ggtatcgtgc cactaatcat tctttttttc tttgtggttt
    11581 cgctggctta tggcatcgct acccgcacaa ttcgacatca ggcggattta ccgcatttaa
    11641 tgattgaacc gatgaaagag atggcgggat ttatcgtgat ggtttttccc ctcgcccagt
    11701 ttgtcgccat gtttaactgg agcaacatgg ggaaattcat cgccgtgggg ctgaccgata
    11761 tactggaaag ttcagggctt agcggcatcc cggcgtttgt cggtctggcg ttgctttcct
    11821 ctttcttatg catgtttatt gccagtggtt ccgcaatctg gtcgattctg gcccccattt
    11881 tcgtaccaat gtttatgcta cttggctttc acccggcatt tgcgcaaatc ctctttcgta
    11941 ttgccgactc atccgtattg cctttagcgc cggtatctcc ttttgttcca ctgtttcttg
    12001 gattcctgca acgctacaaa ccagacgcga aactgggtac ttactattcg ttagtcttgc
    12061 cctatccgct tatctttttg gtggtatggc tgctgatgtt gctggcgtgg tatcttgttg
    12121 gtctgccgat aggtccgggg atttacccac gtttgtctta agagagaacg gatgctgaga
    12181 ttacttgaag aaaaaattgc cacgccactg ggtccactgt gggtgatttg cgatgagcaa
    12241 tttcgcctgc gggcggttga atgggaagag tacagcgaac gcatggtgca gctgctggac
    12301 atccattatc gcaaagaagg ctatgagcgc atttctgcca ccaacccagg cggtttaagc
    12361 gacaagctta gtgattattt tgccggtaat cttagcatta ttgatacgct tcccactgcc
    12421 acagggggga cgccatttca gcgcgaagtc tggaaaactc tacgcactat cccctgcggg
    12481 caggtaatgc attacggcca actggctgaa caattgggcc gccctggcgc ggcgcgtgcc
    12541 gttggtgcgg caaacggatc gaatcccatc agcatcgtcg taccttgcca tcgggttatt
    12601 ggccgaaacg gcaccatgac cggatatgca ggcggagttc agcgaaaaga gtggttattg
    12661 cgccacgaag gttatctttt gctgtaaaca ttaaacaatt tgtgccagct tgttcacact
    12721 tttatgtaaa gttaccctta acaacttaat ggttttcaaa tagatagaca tatatttaca
    12781 tctaatatcg gaattctctg ctgttaaggt ttgcttagac ttacttgctc cctaaaaaga
    12841 tgttaaaatt gacaaatatc aattacggct tgagcagacc tatgatcccg gaaaagcgaa
    12901 ttatacggcg cattcagtct ggcggttgtg ctatccattg ccaggattgc agcatcagcc
    12961 agctttgcat cccgttcaca ctcaacgaac atgagcttga tcagcttgat aatatcattg
    13021 agcggaagaa gcctattcag aaaggccaga cgctgtttaa ggctggtgat gaacttaaat
    13081 cgctttatgc catccgctcc ggtacgatta aaagttatac catcactgag caaggcgacg
    13141 agcaaatcac tggtttccat ttagctggcg acctggtggg atttgacgcc atcggcagcg
    13201 gccatcaccc gagcttcgct caggcgctgg aaacctcgat ggtatgtgaa atcccgttcg
    13261 aaacgctgga cgatttgtcc ggtaaaatgc cgaatctgcg tcagcagatg atgcgtctga
    13321 tgagcggtga aatcaaaggc gatcaggaca tgatcctgct gttgtcgaag aaaaatgccg
    13381 aggaacgtct ggctgcattc atctacaacc tgtcccgtcg ttttgcccaa cgcggcttct
    13441 cccctcgtga attccgcctg acgatgactc gtggcgatat cggtaactat ctgggcctga
    13501 cggtagaaac catcagccgt ctgctaggtc gcttccagaa aagcggtatg ctggcagtca
    13561 aaggtaaata catcaccatc gaaaataacg atgcgctggc ccagcttgct ggtcatacgc
    13621 gtaatgttgc ctgatttttc cgcataactc actatccttc tgtcatatca ttaaattttt
    13681 ctgatttatt gatctggcag aaggttcatc actgtttcat tcaccagata tgggttaatc
    13741 ttttaattac aaactgcgtt gacagttgtt gtaaggagac cctgtatggc tatgtatcag
    13801 aacatgctcg ttgttatcga tcctaaccag gacgaccaac cagcattgcg gcgagctgtt
    13861 tatttacatc aacggattgg tggcaaaatt aaagcctttt tgccgatcta tgacttctca
    13921 tacgaaatga ccaccctgct ctccccggac gaacgtaccg ctatgcgtca gggcgtcatc
    13981 agccagcgta cagcctggat ccacgagcag gcaaaatatt atctcaatgc tggcgttccc
    14041 attgaaatta aagtggtctg gcataaccgt cctttcgaag ccatcattca ggaagtgatc
    14101 agcggcgggc acgatttggt gctaaaaatg gcgcaccaac atgaccgtct ggaagcggtg
    14161 atttttacgc caacggactg gcatctgtta cgcaaatgcc caagcccggt gtggatggtg
    14221 aaagaccagc cgtggccgga aggaggtaag gcgctggtgg cggtgaatct cgccagtgaa
    14281 gagccgtatc ataatgcgct caatgaaaaa ctggtcaaag agacgatcga actggcagaa
    14341 caagtcaacc ataccgaagt tcatctggtt ggcgcttacc cagtaacgcc aattaatatc
    14401 gcgattgaac tgccggaatt tgagccgagc gtttataacg atgccattcg tgggcaacat
    14461 ttgctggcaa tgaaagccct gcggcagaaa ttcggcatta atgaaaacat gacgcacgta
    14521 gaaaaaggtc tgcccgaaga ggttattcct gatttggcgg agcacttaca ggcggggatt
    14581 gtggttctgg gcacggtagg acgcaccggt atttcagcag cattcctcgg caacacggcg
    14641 gaacaggtga ttgatcatct tcgctgcgat ctgttggtca ttaaacctga ccagtatcag
    14701 acacccgttg aactggatga cgaagaagac gattaacgac tcttcaataa ctgacaaggc
    14761 ccgagttatc gggcctattt tttacttact gctcttcgcc gccaaggaaa taaattccca
    14821 acgggattgc cagcaacaca gtaaagacca ggctataaac aaaaatcatc gatgactgaa
    14881 tcacatacat tgatgtcgtc cactcagaca gcggaatatt gtactgttct ataacgccgc
    14941 cgatagtcgc gcgagtcaca accgacgccg caataatgac aaaccgccaa caaaccaaag
    15001 agaaatttct tccctttcgc tgacttcagt ttcgccataa tcatgatgta atccttgtgc
    15061 agacaaattt cttgttacct aaacgtaaca ataccatgac atttcaccgt tgtctgctct
    15121 gaaaacctca cctcttagct ggatttacaa tcaacgcatt gattagtt
//
LOCUS       DADLFA010000095        15120 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000095 DADLFA010000000
VERSION     DADLFA010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15120
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.095"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..421
                     /locus_tag="JQH65_002474"
     CDS             <1..421
                     /locus_tag="JQH65_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY6139857.1"
                     /translation="LMNNSDARKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRL
                     AEFGGLRHKAYRLGGDEFAMVLYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTL
                     SIGYAMTIEHASAEKLQELADHNMYQAKHQRAEKLVR"
     gene            437..919
                     /locus_tag="JQH65_002475"
     CDS             437..919
                     /locus_tag="JQH65_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HAY6139858.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(996..1343)
                     /gene="rplS"
                     /locus_tag="JQH65_002476"
     CDS             complement(996..1343)
                     /gene="rplS"
                     /locus_tag="JQH65_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAY6139859.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(1385..2152)
                     /gene="trmD"
                     /locus_tag="JQH65_002477"
     CDS             complement(1385..2152)
                     /gene="trmD"
                     /locus_tag="JQH65_002477"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAY6139860.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(2183..2731)
                     /gene="rimM"
                     /locus_tag="JQH65_002478"
     CDS             complement(2183..2731)
                     /gene="rimM"
                     /locus_tag="JQH65_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAY6139861.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(2750..2998)
                     /gene="rpsP"
                     /locus_tag="JQH65_002479"
     CDS             complement(2750..2998)
                     /gene="rpsP"
                     /locus_tag="JQH65_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAY6139862.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(3135..4496)
                     /gene="ffh"
                     /locus_tag="JQH65_002480"
     CDS             complement(3135..4496)
                     /gene="ffh"
                     /locus_tag="JQH65_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAY6139863.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            4588..5454
                     /locus_tag="JQH65_002481"
     CDS             4588..5454
                     /locus_tag="JQH65_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311501.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HAY6139864.1"
                     /translation="MQRLEQRSPDAILLLFLIAQTVDITMPVFALLALVAYSVSLALI
                     VPGLLQKNGGWRRMAIISAVIALVCHAIALEARILPDGDSGQNLSLLNVGSLVSLMIC
                     TVMTIVASRNRGWLLLPIVYAFALINLALATFMPNEYITHLEATPGMLVHIGLSLFSY
                     ATLIIAALYALQLAWIDYQLKNKKLAFNQEMPPLMSIERKMFHITQIGVVLLTLTLCT
                     GLFYMHNLFSMENIDKAVLSIVAWFVYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLA
                     YFGSRIVQQLIS"
     gene            5475..6761
                     /locus_tag="JQH65_002482"
     CDS             5475..6761
                     /locus_tag="JQH65_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAY6139865.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(6816..7409)
                     /gene="grpE"
                     /locus_tag="JQH65_002483"
     CDS             complement(6816..7409)
                     /gene="grpE"
                     /locus_tag="JQH65_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAY6139866.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            7532..8410
                     /gene="nadK"
                     /locus_tag="JQH65_002484"
     CDS             7532..8410
                     /gene="nadK"
                     /locus_tag="JQH65_002484"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HAY6139867.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCKKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            8496..10157
                     /gene="recN"
                     /locus_tag="JQH65_002485"
     CDS             8496..10157
                     /gene="recN"
                     /locus_tag="JQH65_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAY6139868.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMAYGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALATQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            10306..10647
                     /gene="bamE"
                     /locus_tag="JQH65_002486"
     CDS             10306..10647
                     /gene="bamE"
                     /locus_tag="JQH65_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HAY6139869.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(10709..10999)
                     /locus_tag="JQH65_002487"
     CDS             complement(10709..10999)
                     /locus_tag="JQH65_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HAY6139870.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(10989..11465)
                     /gene="ratA"
                     /locus_tag="JQH65_002488"
     CDS             complement(10989..11465)
                     /gene="ratA"
                     /locus_tag="JQH65_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HAY6139871.1"
                     /translation="MILFVGFLLMEIVMPQISRTALVPYSAEQMYQLVNDVQSYPQFL
                     PGCTGSRILESTPGQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGG
                     WKFTPLSQEACRIEFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            11597..12079
                     /gene="smpB"
                     /locus_tag="JQH65_002489"
     CDS             11597..12079
                     /gene="smpB"
                     /locus_tag="JQH65_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAY6139872.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            12294..12656
                     /gene="ssrA"
                     /locus_tag="JQH65_002490"
     tmRNA           12294..12656
                     /gene="ssrA"
                     /locus_tag="JQH65_002490"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(12817..15120)
                     /locus_tag="JQH65_002491"
                     /pseudo
     CDS             complement(12817..15120)
                     /locus_tag="JQH65_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         3709 a         4060 c         3544 g         3807 t
ORIGIN      
        1 gttgatgaac aattccgatg cccgaaaaac gtcggcgtta ctatttcttg atggcgataa
       61 tttcaaatat atcaatgata cctggggtca tgcgacgggc gatagggtct tgattgaaat
      121 cgcaaaacgg ttagctgaat ttggcgggct gcgacataaa gcataccgcc tgggcggcga
      181 tgaattcgct atggtgctct atgatgtaca gtcggaatct gaagtgcaac agatatgctc
      241 agcactgaca caaatcttta atctcccgtt tgatcttcat aatggccatc agaccaccat
      301 gacattaagc attggttacg cgatgaccat tgagcacgcc tctgcggaaa aattacaaga
      361 gcttgccgat cacaatatgt atcaggccaa acaccagcgt gccgaaaagc tggtgagata
      421 acaaggatat atcgatatga taaagcacct ggtagccccc ctgattttca cctcactaat
      481 actgactggc tgccagtccc ctcagggaaa gtttactcct gagcaagtcg ccgctatgca
      541 atcttatgga tttactgaat ccgccggcga ctggtcgctg ggcttatcag atgccattct
      601 gttcgcaaaa aatgactaca aattgctccc ggaaagccag caacagatcc aaaccatggc
      661 agccaaattg gcctcgacag ggctaacaca tgcccgtatg gatggacaca ccgataacta
      721 tggtgaagac agttacaacg aaggcttatc attgaaacgg gcgaatgtcg tggccgatgc
      781 atgggctatt ggtggacaaa ttccacgcag caatctcacc acacagggtt taggaaaaaa
      841 atatcccata gccagtaaca agaccgccca gggccgcgcc gagaaccgcc gcgtcgcagt
      901 ggtgattaca accccttaaa acacgagcaa acagacaaaa aaagggccag ccaattggcc
      961 agcccttctt aacaggatgt cgcttaagcg aaatcttagt taagacgctc tttgatacga
     1021 gcagccttac cagtacgctc acgcaggtag tacagtttag ctttacgaac agcaccacga
     1081 cgtttgacag aaatgctgtc aactaccgga gagtgagtct ggaagacacg ctcaacgcct
     1141 tcgccgttgg aaattttacg aacagtgaat gcagagtgca gaccgcggtt acgaatagcg
     1201 ataaccacgc cctcgaatgc ctgcagacgt tttttggaac cttcaacaac ccatactttc
     1261 acttccacgg tatcacccgg acggaaggaa ggtacgtcct gcttcatctg ctcttgttca
     1321 agttgcttaa taatgttgct cataatttaa tctcttatcc tgggtaaact gatatctcgg
     1381 gggcttacgc catcccatca tgtttatgtt gctgttgtgc gtgttccgtt ttgaactccg
     1441 ccagcaacct tgcttgctct tcagtcagag ccaggttttc cagaagttca ggtcttctaa
     1501 gccaggtacg gcccagcgac tgtttcaaac gccagcgacg tatctcggca tggttgcccg
     1561 acagtaacac tggcggaact tccatccctt ctaacacctc aggccgcgta tagtgcgggc
     1621 aatccagcaa tccttcagca aaggaatctt ccgttgccga ggcttcatgt cccagtaccc
     1681 ccggaataaa ccgggaaacg gagtcaatca gcgtcattgc tggtaactca ccaccactga
     1741 gaacgtaatc gccgattgac cattcttcgt caatttcggt ttggatcacg cgctcatcta
     1801 taccttcgta gcgaccgcac accagaatca atttttgatt cgttgccaat tcgctgacgc
     1861 ccgcttgatc aagcttgcgt ccctgtggtg acagataaat cacctttgcg ccttcacccg
     1921 ccgcggcttt tgctgcatga atggcgtccc gcaagggttg caccatcatt aacatccccg
     1981 gtccgccgcc gtaaggacga tcgtccacgg tacggtgccg gtcatgcgtg aagtcgcgag
     2041 gactccagct ctggatgctc agcaggccat ttttaactgc ccggccagtt accccgtaat
     2101 cggtaattgc gcggaacatt tcaggaaaca ggctaattat gccaatccac atagcgccgt
     2161 cttttaccgt ttatccggtg gtttaaaaac caggatccca atctacttcg attgaacgag
     2221 tagtgagatc gactttcttg ataacctgcc catcgaggaa cggtacgaga cgttccttga
     2281 taccaaacgc atctttcagg tttgccttaa tgacgagaac gtcattagat ccggtttcca
     2341 tcatatcgac gactttaccg agatcgtagc cttcagtggt tactacctgg cagcccatca
     2401 ggtctttcca gtagtagtca ccctcttcaa gctgaggcaa ctgcgatgaa tccacgacaa
     2461 tttcacaatt cgtcagcagg ttcgccgcat cacgatcgtc aacgcctttc agcttgatga
     2521 tcatgtcctg attgtggtgc ttccagcttt ccagctggac ttgctgccac tgacccgcct
     2581 tctggataaa ccagggctga tagtcaaaaa tgctttcggc gtcttcggtg gaagaaaaca
     2641 ctctgagcca cccacgaata ccgtaagacg aacccatttt tcccaaaacg atgggatcaa
     2701 caggtgcttg cgcggtgagt tgtttgctca tcatgaccac cgtgacagat taagctgctt
     2761 tgtttacttc tttgatcagc gcagcaacgc gatcagaaat agttgcgccc tggccaaccc
     2821 agtgagcgat gcgatccaga tccaggcgag tgccttcttc tttttcgcta gcgattgggt
     2881 tgaagaaacc aacgcgctcg atgaagcgac cgttgcgtgc attacggctg tcagcgacaa
     2941 caacctggta gaacggacgc tttttagcgc cgtgacgtgc taaacgaata gttaccataa
     3001 catcctcttg tgtgaataaa acaaccggac cccatcgagg aacggagtcc ggtgtcatat
     3061 taaaagcccg aaaattttac tgatttctgg agaaaatgca atcagcagtt aataactctg
     3121 tcgcataagg tcatttagcg accaggaaat cccggcggca tcatcccctt catgcttctc
     3181 atcatcttcg ccattccgcc cttcttcatt ttcttcatca tgcgctgcat gtcgtcgaac
     3241 tgtttcagaa gacggttaac gtcctgcacc tgcataccgc aaccggcagc aatacggcgt
     3301 ttacgcgaac ctttgatgat ttctggctta gcgcgctctt tcatcgtcat cgagttgatg
     3361 atggcttcca tacgcaccag cactttatcg tccatctgtg acttgacgtt atccgggatc
     3421 tgccccatgc ccggcagctt gcccatcagg ctggccatgc cgcccatatt tttcatctgg
     3481 cgcagctgct caagaaagtc gttgagatcg aagccgtcac cttttttcag cttgctggct
     3541 aatttctctg cctgcgcgcg gtcaactttg ctttcgatat cttcgatcag cgacagtacg
     3601 tcacccatgc cgagaatacg cgacgcgatg cggtccggat ggaacggctc cagcgcctca
     3661 gttttctcgc caacaccgag gaacttgatc ggtttgccag tgatgtgacg aatagagagc
     3721 gccgcaccgc cgcgggcatc gccgtccact ttggtcaaca ctacgccggt aagcggtaac
     3781 gcttcattga atgcttttgc cgtattggcc gcatcctgac cggtcatggc gtcaaccaca
     3841 aacagggttt caaccgggtt aatcgacgca tggacttgtt tgatctcgtc catcatcgct
     3901 tcgtcaacgt gcagacgacc agcggtatcc accagcagca cgtcgtagaa tttcagtttg
     3961 gcttctttca gcgccgcgtt aacgatatct accggcttct gaccaacatc agaagggaag
     4021 aaatcaacgc ccacctgctc tgccagcgtc tcaagctgtt tgattgccgc cgggcgataa
     4081 acgtcggcag aaaccaccag cactttcttc ttgtgcttct cgcgcaggaa cttaccgagt
     4141 ttaccaacgc tggttgtttt accggcacct tgcaggcccg ccatcagcac gaccgcaggc
     4201 ggttgcgccg ccaggttcag ggtctggttc tcttcgccca tcgccgcaac cagttcgtta
     4261 cggacgattt tgacgaactc ctgccccggc gtcaggctct tattaacttc atgaccaacc
     4321 gctttctctt ttacgcgatt gataaactca cgcactaccg gcagagcgac atccgcctcc
     4381 agcagcgcca tgcgcacttc gcgcagcgta tcttttacgt tgtcttcagt taggcgtcca
     4441 cggccactga tattgcgcag cgtgcgcgac aaacgatcgg ttaaattatc aaacattgtc
     4501 tctcgcctgg ggtggaaacg gttggccgca atcgcgacac atcatcagta ttttgccgca
     4561 gtataacatg aaggcgtctt tgttgttatg caacggttgg agcagcgttc acctgacgct
     4621 atactgcttc tctttcttat tgctcaaact gttgacatca ctatgcccgt ttttgctctg
     4681 ctcgcgcttg tcgcctactc cgtcagtctt gcgctgattg ttcccggtct gctgcaaaaa
     4741 aacggcggct ggcggcgcat ggctattatt tctgcggtca ttgcgctggt ctgccacgca
     4801 atcgctctgg aagcccgcat cctgcccgac ggtgatagcg gacaaaacct cagcctgctg
     4861 aacgttggtt cattggtcag tttgatgatc tgtacggtaa tgaccattgt ggcttctcgc
     4921 aatcgtggct ggttgctgct acccattgtc tatgcctttg cgcttatcaa cctggcgctg
     4981 gcgaccttca tgcccaatga atacatcacc catctggaag caacgcctgg gatgctggtg
     5041 cacattggct tatcgctctt ttcctatgcc acgctaatta tcgccgccct gtacgcgctg
     5101 caactggcgt ggattgatta ccaactgaag aacaagaagc tggcatttaa ccaggaaatg
     5161 ccgccattga tgagtatcga gcgtaaaatg ttccacatca cgcagattgg cgtggtgctg
     5221 ctaacgctca cgctttgcac tggcctgttc tacatgcaca acctatttag catggaaaat
     5281 atcgacaagg ctgtgctctc tatcgtggcg tggtttgtct atattgtgct gctgtgggga
     5341 cattaccatg aaggatggcg tggacgccgc gtcgtctggt ttaacgttgc gggcgcggta
     5401 attctgacac tggcctactt cggcagccga attgtccagc agttaatcag ctaaacccag
     5461 aaaaggagtt tcccctggaa cacatttcta ctactacgtt gatcattatt ctgatcatca
     5521 tggtggtcat ttcagcctat ttttccgggt ccgaaaccgg aatgatgacc ctcaaccgct
     5581 atcgtctgcg acatatggcg aaacagggta atcgctcggc caaacgcgtc gaaaaattgc
     5641 tgcgtaagcc agaccgcctg ataagcctgg tgttaatcgg caataacctg gtcaatattc
     5701 ttgcctccgc gctcggcact attgttggga tgcgtttgta cggcgatgcg ggcgtggcaa
     5761 ttgcgactgg tgtgctgact tttgtcgtac tggtatttgc tgaggtattg ccgaaaacca
     5821 ttgccgcgct gtacccggaa aaagtcgctt atccgagtag ttttctgctg gctccgctgc
     5881 aaattttgat gatgccgctg gtctggttgc tgaatgctat cacccgtatg ctgatgcgca
     5941 tgatgggtat caaaaccgat atcgtggtta gcggctcttt gagcaaagaa gagttgcgca
     6001 ctatcgtgca cgaatcacgc tcacaaattt cccgtcgcaa tcaggatatg ctgctgtcgg
     6061 tgctcgatct ggaaaaaatg accgttgatg acatcatggt gccgcgcagt gaaattatcg
     6121 gtattgatat caacgatgac tggaaatcga ttctgcgcca actctcccac tcacctcacg
     6181 ggcgcatcgt gctctaccgt gattcgctgg acgacgccat cagtatgctg cgagtacgtg
     6241 aagcctggcg attgatgtcg gagaaaaaag agttcaccaa agaaaccatg ctgcgcgccg
     6301 cggacgagat ctattttgtg ccggaaggta cgccgctcag cacgcagttg gtaaagtttc
     6361 agcgcaacaa aaagaaagtc ggcctggtcg tcaacgagta tggagacatt caggggctgg
     6421 tgacggttga agatattctg gaagagattg tcggcgattt cactacgtcg atgtcgccaa
     6481 cacttgccga agaggtcacg ccgcaaaacg acggttcggt gattatcgat ggcaccgcca
     6541 acgtgcggga aatcaacaaa gcctttaact ggcatctacc ggaagatgat gcccgcacgg
     6601 ttaatggcgt cattcttgag gcactggaag agatccctgt cgcaggcacc cgcgtgcgta
     6661 ttggcgagta cgatatagat attctcgacg tacaggacaa tatgattaag caggtaaaag
     6721 tttttcctgt gaaaccgctg cgcgagagtg tggcggagta acgaaaacgg ccctgcattc
     6781 gcatgcaggg ccgtgaatta ttacgaaaac agaaattacg cttttgcttt cgctacagtt
     6841 accatcgccg cacgaatcgt acgaccattc agcgtataac ccttctgcat aatgcccagt
     6901 acgttacctg gcgcaacgtc atcagattcc accattgcga tagcctgatg cacattcggg
     6961 tccagcggca cgttagtttc ggcgatcact tcaacaccaa acttacgcac aacatccagc
     7021 atcgacttca gcgtcagctc aatgccttca accatcgcag acatatccgg gttagcttta
     7081 tcagccactt ccagcgcacg atccaggcta tcaatcaccg gcagcaattc gttgatgaat
     7141 ttctccagcg caaatttgtg ggctttttca atatccagtt cagtacgtcg acgcaggttt
     7201 tccatttcgg cttttacacg caaaatgccg tcacgttcac gggtctgggc ttcagccagc
     7261 tgagcttcga gattcgcaat tttttcatcg cgcggatcca cctgctcagc agaagcttct
     7321 ggctcaactg cctcaatctc ttcgtgctga tccatgataa tttcttccgg ggcttgcccc
     7381 tcaggcgttt tctgttcttt actactcatg aatttctccg cgtttttttc gcattcatct
     7441 cgctaacttc gcttattatg gggatcagtt tcagggtttc aagggaagca ctcacattgt
     7501 catcaatctt cgcaacaagg acctcggaaa aatgaataat catttcaagt gtattggcat
     7561 tgtgggacac ccacggcacc ccactgcact gacaacacat gaaatgctct accgctggct
     7621 gtgcaaaaaa ggttacgagg tcatcgttga gcaacaaatc gctcacgaac tgcaactgaa
     7681 gaatgtgaaa actggcacgc tcgcggagat tgggcaacta gctgatctcg cggtagtcgt
     7741 tggtggcgac ggtaatatgc tgggcgcggc acgcacactc gcccgttacg atattaaagt
     7801 tattggaatc aaccgtggca acctgggttt cctgactgac cttgaccccg ataacgccca
     7861 gcaacagtta gccgatgtac tggaaggcca ctacatcagc gagaaacgtt ttttgctgga
     7921 agcgcaagtc tgtcagcaag attgccagaa acgcatcagc accgcgataa atgaagtggt
     7981 gcttcatcca ggcaaagtgg cacatatgat tgagttcgaa gtgtatatcg acgagatctt
     8041 tgcgttttct cagcgatctg atggactaat tatttcgacg ccaacaggct ccaccgccta
     8101 ttccctctct gcaggcggtc ctattctgac cccctctctg gatgcgatta ccctggtgcc
     8161 catgttcccg catacgttgt cagcacgacc actggtcata aacagcagca gcacgatccg
     8221 tctgcgtttt tcgcatcgcc gtaacgacct ggaaatcagt tgcgacagcc agatagcact
     8281 gccgattcag gaaggtgaag atgtcctgat tcgtcgctgt gattaccatc tgaatctgat
     8341 tcatccgaaa gattacagtt atttcaacac attaagcacc aagctcggct ggtcaaaaaa
     8401 attattctaa ttttacgcca gcctctttac tgtatataaa accagtttat actgtacgca
     8461 ataacagtaa tggtttttca tacaggaaaa cgactatgtt ggcacaactg accatcagca
     8521 actttgctat cgttcgtgag cttgagattg attttcatag cggcatgacc gtaataactg
     8581 gcgagaccgg cgcgggtaaa tctattgcaa tagatgccct cggtctttgt ctcggtggtc
     8641 gcgctgaagc cgacatggtg cgtaccggcg ctgctcgcgc tgacctgtgc gcccgttttt
     8701 ctctgaaaga tacgccagcg gctctgcgct ggctggaaga aaatcagctt gaagacgggc
     8761 atgaatgttt gcttcgtcgc gtaatcagca gcgatggtcg ctcccgtggt ttcatcaacg
     8821 gtacagctgt tcctctgtca caactgcgcg aactgggtca gttgctgatt cagatccatg
     8881 gtcagcacgc tcatcaatta ctcaccaaac ctgagcacca aaaattcctg cttgatggct
     8941 atgccaatga aacctctcta ctgcaggaaa tgaccgcacg ttatcagttg tggcatcaaa
     9001 gctgccgtga cctcgcgcat catcaacagt taagtcagga acgcgccgcc cgtgcggaac
     9061 tgctgcaata ccaattaaaa gaacttaacg aatttaatcc gcagcccgga gagtttgaac
     9121 aaatcgacga agagtacaaa cgtctggcga acagcggtca attgctgacc accagccaga
     9181 atgcattggc attaatggcc tacggtgaag acgcaaacct gcaaagtcag ctttacacgg
     9241 ctaaacaact ggtgagcgaa ttgattggca tggacagcaa actgtccggc gtacttgata
     9301 tgctggaaga agctaccatc cagattgctg aagccagcga tgaactgcgc cactactgcg
     9361 atcgtctgga tctcgatccc aaccgactat ttgaacttga acagcgcatc tcaaaacaga
     9421 tttcgctggc acgtaaacat cacgtcagcc ctgaggcatt gccacagtat taccagtcgc
     9481 tactggaaga acagcagcaa ctggacgatc aggccgactc acaagaaacg cttgcgctgg
     9541 cggtaacgaa acatcatcag caggcactgg aaatcgcgcg cgcattacac caacaacgcc
     9601 agcaatatgc agaagaactt gcacagctga tcaccgacag tatgcatgcg ctctcaatgc
     9661 cgcatgggca gtttacgatc gatgttaaat ttgacgagca tcacctgggc gctgacggtg
     9721 ccgatcgtat tgagtttcgg gtaaccacca acccaggtca gccaatgcag cctattgcca
     9781 aagtcgcatc cggtggtgaa ttgtcccgca tcgcactggc aacccaggtc atcacggcgc
     9841 gtaaaatgga aaccccggca ctgatttttg atgaagtgga tgtagggatt agcggtccaa
     9901 cagcggcagt tgtcggcaaa ctgctgcgtc aactcggcga atcaactcag gtgatgtgtg
     9961 ttacccacct gccacaagtc gcgggatgtg gtcatcaaca ctattttgtc agcaaagaaa
    10021 ccgatggtgc gatgacagaa acgcatatgc aatccctgaa taaaaaagcg cggttacaag
    10081 agctggcgcg cctgcttggt ggcagtgaag tcacacgtaa tacactggcg aatgcgaaag
    10141 aactgcttgc agcgtaaact tttttcctgc ttcacggtca gagtaaacag caaaacgccg
    10201 taagaccgga aagcaaaagg ttttaaagtg atgaaaggtc tattatcatc ggcatattac
    10261 agatgagcca cgtactgcta gggcccgaaa aggaatcaaa tcactatgcg ctgtaaaacg
    10321 ctgactgctg cagcagcagt actattgatg ttgaccgcag gctgttccac tctggagcga
    10381 gtggtttacc gtcctgacat caaccagggg aactatctga ccgctaacga cgtatccaaa
    10441 atacgtgttg gcatgacgca acaacaagtt gcgtacgcat tgggtacacc gctgatgtcc
    10501 gatccatttg gtacgaatac ctggttctat gtcttccgcc agcaaccagg tcatgaaggt
    10561 gtaacgcagc aaacgctgac gctgaccttt aacagtagcg gtgtgttgac caatattgat
    10621 aacaaacctg cgctgagtgg taactaataa taaagttgct ctcaaagacg ttaaaaaagg
    10681 tgctcaatga gcaccttttt tctgtctgtt atttattcgc tgatttttct gctcgttgcc
    10741 tgcgaagctc tttcggatcg gcaatgagag ggcgataaat ctccacccga tcgccatcat
    10801 gcacactatc gcttagtttt gccggacggc tgtaaatgcc gactttgttt ttagttaaat
    10861 cgatatcggt acgcaattcc agcaagccac tggcgcgaat agcttcttca accgtcgcgc
    10921 cctcctgcag cgtcacgcgc tgcaggtact gcttctcagg tagcgcataa gccacctcaa
    10981 cggcaatttt acctggcact gtaaacctct ttcgcacgaa ccgtaaaagc ctggaccata
    11041 ttagacgcca gctctttaaa cacgcgacca aaggcgagtt caatcaactt attggtaaac
    11101 tcaaagtcga gatgaaactc gatacgacac gcctcctggc tcagcggcgt aaacttccat
    11161 ccaccaatca atttcttgaa cggcccatcc accagattca taagaatact ttggttactg
    11221 gtcaactggt tgcgggtagt aaacgttttg ctgatcccag ccttagagac atctaccgcc
    11281 gcagtcatct gcccaggagt ggactccaga atccgacttc cggtacaacc cggcaaaaac
    11341 tgaggataag actgaacgtc attcactaac tgatacattt gctccgcgct gtagggtacc
    11401 agtgcggtcc ggctaatctg aggcataact atttccatca acaaaaatcc aacaaataat
    11461 atcatttatc ctgctgtaaa aaaaacgcta tcccggcgct gggtaacatc gggttcatgc
    11521 taagatagag ccttgtcccc cgcaggattg atatggggtg ttttcgattt cagattaccg
    11581 atgattcacg acgcttatga cgaagaaaaa agcacataaa cctggttcag cgaccatcgc
    11641 gcttaacaag cgcgcccgtc acgaatactt tatcgaagaa gagttcgaag cgggacttgc
    11701 cctgcaaggc tgggaagtta aatccctgcg cgcaggaaaa gccaatatca gcgacagcta
    11761 cgtccttctg cgtgacggag aggcatttct gtttggcgct aacatcacgc caatggccgt
    11821 ggcctccacg catgtggtgt gcgatcctac ccgtacccgc aagttacttc tcaaccagcg
    11881 cgaactggac tcattgtacg gtcgcgtcaa tcgagaaggc tataccgtag tggcgctctc
    11941 cctgtactgg aaaaatgcct ggtgcaaagt gaaaatcggc gtcgccaaag gtaaaaaaca
    12001 gcacgataaa cgttcagata tcaaagaacg cgaatggcag gtggataaag cacgtatcat
    12061 gaaaaacgcc caccgttaaa cctgcactcc aattattgac cagttcctca ccgcgcctcc
    12121 ctctccggcg gcgcgaatga acatcttatt ggctatcaca tccgacacaa atgttgccat
    12181 cccattgctt aatcgaataa aaatcaggct acatgggtgc taaatcttta acgataacgc
    12241 cattgaggct ggtcatggcg ctcataaatc tggtatactt gccgttacac attggggctg
    12301 attctggatt cgacgggatt tgtgaaaccc aaggtgcatg ccgaggggcg gttggcctcg
    12361 taaaaagccg caaaaaatag tcgcaaacga cgaaaactac gctttagcag cttaataacc
    12421 tgcttagagc cctctctccc tagcctccgc tcttaggacg gggatcaaga gaggtcaaac
    12481 ccaaaagaga tcgcgtggaa gccctgcctg gggttgaagc gttaaaactt aatcaggcta
    12541 gtttgttagt ggcgtgtccg tccgcagctg gcaagcgaat gtaaagactg actaagcatg
    12601 tagtaccgag gacgtaggaa tttcggacgc gggttcaact cccgccagct ccaccaatca
    12661 tgattggacg gtataaggac aacaccaata aaaacaggag gttagcagtc tcagcaggac
    12721 accgaccaga cggtgaagag acataaaagg atacgcaaag gagccgcggc tcctggtgac
    12781 atgaaagccc acagatgtgg gctttttcgt tgatggtcag aacgaccagt tcacaccagc
    12841 taccccgttc cacggggatt ccacaccggc accatggcta taccccaccc caagatgccc
    12901 gcttaacgta ctgctgaatg aggctttaat acctgcctgg tatattccac gtctgcccga
    12961 caaatcattg acgaaattac cgtcactatt cactttcacc cggttatcat cgacaaattc
    13021 tttgcgcaca gccgccttca gccacggctc aacttccata ccgttcccca gacgcatgtt
    13081 gtaactcagc gttgcaccca gttcacgata taaacctgcg ggtatcgact gatttcgatt
    13141 ccatgccatt ggataaatga tattcagggt tatcagcggt gaacccccgt taacgatgca
    13201 tacggcgtca ggttccagtt accatcggta aatcgcatcc cggtttcaat gtgaccgccc
    13261 agcccgttgc tgcgataact gccgttggtg gctccaccgc tgccaaccgt ggagtcgcga
    13321 gcttcggtac ctgctaatac caataaatta ccgccatttt ccagcaacat attggtcgct
    13381 aaattgccgg aaatggaaaa agtgccgtac tggtgagtac cgctgatttc aataccgtta
    13441 gccgtgctcg tctggagagc ggcaccgctg ttctggacga tatctgtcgc tttgccatta
    13501 tcgttaactg tcagcgtacc gccttcattg atctttgttt ttattgcctc tccgttagct
    13561 gaaactgttt gtattccgcc gtcgttaatc gttgtctcat tcgccacccc ctcgacgatt
    13621 tgttcgccgc cggtgagcgt cgtgcctgtc gcagtggctt ttgttttgac gatctcccgt
    13681 ccgcccgtat tgacctgtgt tttgtcagaa gaggtgtctg actccacggt taacacgccg
    13741 ccatttgcca gcaggatatt gttcgccgca ccctgctcga tgctgaacgc gacgccatcc
    13801 gcgcgtgttc ctgtgacccg cgtcgccctg gtggttgcaa ccaaagcgcc ctggctactc
    13861 tgctgtatcc ccgttgcgct gcctttctcc cgcacatcga gtgtgccgcc gtcattaagc
    13921 accgagtttt cagccagacc gccctcatta actacctgtg aacccccatt aataatggaa
    13981 ccttccgctg tcccgtttgc cataatttgt tgtaggccag agacgatatc ggtattgatt
    14041 gccttaccat aattctgaac ggtttgcgtg ccaccattga tgtgtgtttt ctctgttgac
    14101 ccaccatcaa caatttgttc accactttcg atatttgcct cagtggctaa tccatatacc
    14161 gtttgcttgc cacctttgat atttgcttta tcagaagtgg cactggcata tattgtttgg
    14221 gtgccagcac tattgagtac agtgccaaca tcttttccat aaacatccat tttgccgttg
    14281 gcattaataa tcgtatcaac tgcccgggaa ccagtgacga ctgttaatga gccagcgttt
    14341 tccagcacta catttttagc ttctgaattc ctgatgtaga aagcatcacc ataactgttg
    14401 gttcctttga taagggttcc ggaagttgtg gaagcaatta atgcgccgcc ggattgttgc
    14461 tcaacatgct tagcctcacc accgtcctga acctccagaa cgccaccatt attaagtctg
    14521 gtggtatctg ttttagcctc cttctggaca atcagcttac cgccagcatc aacggtagta
    14581 tttttcgccg aggttttagc cactaccgtc agttcgccgg tattttccag cacaacataa
    14641 ttagcctccc ctccgggtaa tagtgaagtg agagagcttg ttgtatcctt cgatatcagt
    14701 ccctgcgccc gtgttggcaa ctaaagcact gcccgtctcc tggttaaccc catgtgctat
    14761 accgccggta tagacaatca gcgagcctcc ggcgctaata ttgctgccag ttgccgtacc
    14821 atctttctca acaacctgcc ggctcccggt ggaaatattt gtcgtgtcag ctttcccgcc
    14881 gctcttgata ttttgtgttc cgctgttgat attggtaccc gtggcaatac catgattatt
    14941 aatattctgt gtaccaccat tgattatggt attggtcgcg tttccggtaa catccattac
    15001 cccgccgtta tctattcggg tcgcatcagc tttagcattc gttaaaactg acattgttcc
    15061 tttatcttta ataatcgtct tatttgccga accatatgcg tttatgtcta aatgaccacc
//
LOCUS       DADLFA010000096        15093 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000096 DADLFA010000000
VERSION     DADLFA010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15093
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.096"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..164
                     /locus_tag="JQH65_002492"
     CDS             <1..164
                     /locus_tag="JQH65_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimH domain protein"
                     /protein_id="HAY6139873.1"
                     /translation="RNGKILATGENVSLGTVNKSKVSLGLSATYGQTGNKVAAGTVQS
                     VIGVTFIYE"
     gene            498..2777
                     /gene="ydeP"
                     /locus_tag="JQH65_002493"
     CDS             498..2777
                     /gene="ydeP"
                     /locus_tag="JQH65_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HAY6139874.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHTASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIEHISGLSQTQIAELADAYAAADRTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3025..3222
                     /gene="safA"
                     /locus_tag="JQH65_002494"
     CDS             3025..3222
                     /gene="safA"
                     /locus_tag="JQH65_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HAY6139875.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
     gene            3297..4058
                     /gene="ydeO"
                     /locus_tag="JQH65_002495"
     CDS             3297..4058
                     /gene="ydeO"
                     /locus_tag="JQH65_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HAY6139876.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHTGMNTGNTMSALAI"
     gene            4460..6142
                     /locus_tag="JQH65_002496"
     CDS             4460..6142
                     /locus_tag="JQH65_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAY6139877.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYNLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            6194..7351
                     /locus_tag="JQH65_002497"
     CDS             6194..7351
                     /locus_tag="JQH65_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HAY6139878.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CSFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(7317..7439)
                     /locus_tag="JQH65_002498"
     CDS             complement(7317..7439)
                     /locus_tag="JQH65_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6139879.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            7654..9339
                     /locus_tag="JQH65_002499"
     CDS             7654..9339
                     /locus_tag="JQH65_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HAY6139880.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     LLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDIFSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVL"
     gene            9377..11749
                     /locus_tag="JQH65_002500"
     CDS             9377..11749
                     /locus_tag="JQH65_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HAY6139881.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVVGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYSGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            11794..14589
                     /locus_tag="JQH65_002501"
     CDS             11794..14589
                     /locus_tag="JQH65_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAY6139882.1"
                     /translation="MEIIMRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGL
                     RYMIYPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETF
                     ESMGLRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAE
                     RGVITEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQR
                     WYQPNNMTFIVVGDIDSKKALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDK
                     ENRVNGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTA
                     RSVKIAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWL
                     KNAVDQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQL
                     RKNQDAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQ
                     AEISSKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIA
                     LANKAVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLIN
                     QRITHSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIV
                     QFTAADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPL
                     TRATDNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIRE
                     QASGAYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQEL
                     NEYQQNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVK
                     QYLSHPVNTYTGVLLPK"
     gene            14951..>15093
                     /locus_tag="JQH65_002502"
     CDS             14951..>15093
                     /locus_tag="JQH65_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY6139883.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IIN"
BASE COUNT         4423 a         3283 c         3283 g         4104 t
ORIGIN      
        1 tgcgtaatgg aaaaatactg gctacgggag aaaatgtctc cctggggaca gtaaataaaa
       61 gcaaagtatc tctgggttta agcgcaacct atggacaaac aggaaacaag gttgcagccg
      121 gaaccgtcca gtcagtcatt ggtgtcactt ttatctatga ataattgtga ttttgatctt
      181 agtctatgcg ctccattcac gaaaattgcg cggataatta ttttgtgaag gctattatcc
      241 tacacctgta agaaaatccg cgcacaattg aagccagagt cttatttttt atattgaaaa
      301 atatgttaat ttaaacacta tgagagatga tgcttaactt atcgtattta atctattgtt
      361 taacgataat agattaaaaa tagaaagaga gtagtcccct actcttatat atccatgttg
      421 gcgataatcc ctttgtatgt actgtgcatc atcgctatta caaatcctaa taattcattt
      481 ccacacagga taagtagatg aagaaaaaaa ttgaatccta ccagggtgct gcaggtggtt
      541 ggggtgctgt taaatccgta gcgaatgcag tacgtaagca gatggatata cgccaggatg
      601 ttattgccat gtttgacatg aataagccag agggctttga ctgtccgggt tgtgcatggc
      661 cagatcctaa gcacactgcg tcattcgaca tttgtgaaaa cggcgcaaaa gcaatcgcct
      721 gggaagtcac ggataagcag gtaaatgcct ctttctttgc tgagaatacg gttcaatcat
      781 tacttacctg gggagatcac gagcttgagg ctgcgggacg tctcactcag cctttgaaat
      841 atgatgccgt cagcgactgt tacaagccat taagctggca acaagctttc gacgaaattg
      901 gcgcacgcct tcaaagctat agtgatccca atcaggttga attctatact tcgggccgca
      961 cttccaatga agctgccttt ctttatcagc tttttgcccg tgaatacggg agcaataact
     1021 ttcccgactg ctccaacatg tgccatgaac cgaccagtgt gggtttggca gcgagtatcg
     1081 gcgtgggtaa agggactgtg ttgctggaag actttgagaa gtgcgattta gtcatttgca
     1141 ttgggcataa ccctggtaca aaccaccccc gcatgctgac ttcgttgcgc gctttagtga
     1201 aacggggagc gaaaatgatc gccatcaatc ctttacagga acgtggcctg gagcgattta
     1261 ccgcaccgca aaacccgttt gaaatgctga cgaactctga gactcagttg gccagtgcgt
     1321 actacaacgt gcgcattggt ggcgatatgg cgttgctcaa ggggatgatg cgcctgttaa
     1381 ttgagcgcga tgatgctgca agtgccgcag gtcggccctc attgcttgat gacgaattta
     1441 ttcaaacgca taccgtcggc tttgacgagc tacgccgtga cgttctcaat tccgagtgga
     1501 aagatatcga acatatttct ggactaagtc agacacaaat cgccgaactg gctgacgcat
     1561 atgccgctgc cgatcgcacc attatctgtt acggaatggg gatcactcag cacgaacatg
     1621 gtacccagaa tgtacagcaa ctggtcaatc tgctgttgat gaaaggtaac attggcaagc
     1681 ctggtgcggg tatctgccca ctacgaggtc actctaatgt acagggcgac cgaaccgtcg
     1741 gtatcaccga gaaaccgtct gcagagtttc tggctcgcct gggtgagcgc tatggcttca
     1801 ccccacctca tgcacctgga catgctgcaa ttgccagcat gcaagcaata tgcacggggc
     1861 aggctcgagc attgatctgc atggggggca actttgcact ggcaatgcca gatcgggaag
     1921 cgagcgctgt accgttaacg caattagatt tggcggtaca cgtagccact aagcttaacc
     1981 gctctcatct gctgaccgca cggcatagct atattctgcc ggttctgggg cgtagcgaga
     2041 ttgacatgca aaaaagcggt gcgcaggcgg taaccgttga ggattcaatg tcgatgattc
     2101 atgcctcgcg tggcgtgtta aaacccgccg gtgtaatgct gaaatcagag tgtgcagtgg
     2161 tcgcgggaat cgcgcaggca gcactacccc agagcgtggt agcctgggag tatctggtgg
     2221 aagattatga tcgcattcgc aatgacattg aagctgtgtt accagagttc gccgactata
     2281 accagcgcat ccgacatcct ggaggttttc acctgataaa tgcagctgct gaaaggcgct
     2341 ggatgacgcc gtcaggtaag gctaatttca ttaccagcaa agggctgtta gaagatccct
     2401 cttcagcgtt taacagtaag ctggtcatgg cgacagtacg cagccacgat cagtacaaca
     2461 cgacgattta tggtatggat gatcgctatc gaggggtatt cggtcaacga gatgtggtct
     2521 ttatgagtgc taaacaagct aaaatttgcc gtgtaaaaaa cggcgaaaga gttaatctta
     2581 ttgcgcttac gccagacggt aagcgcagct cacgccgcat ggatagatta aaagtggtca
     2641 tttaccctat ggctgaccgc tcactggtga cctattttcc agaatcgaat cacatgctaa
     2701 ctcttgataa ccacgatcca ttaagtggca ttcctggtta taaaagtatt ccggttgaat
     2761 tagaaccatc aaattaattc gcctcctcat ttcttctgct gtcatccgca cagcagaaga
     2821 atttctcatt gactattatc gcgcaatttg ctcacattga tcaaattaaa ccacacacta
     2881 taagatataa acctctgcct acagctgtaa gaaactccgc tcagtactga agtgccagtc
     2941 ctatttcctc ttttctccag cctattatat taagcatact gattaacaat ttttaacgtt
     3001 atccgctaaa taaacatatt tgaaatgcat gcgaccacag tgaaaaacaa aatcacgcaa
     3061 agagacaact ataaagaaat catgtctgta attgtggttg tcttattact gacccttacg
     3121 ttgatagcca ttttttcggc aattgatcag ctgggtattt cagaaatggg tcgcattgca
     3181 agagatctta cacatttcat tatcaatagt ttgcaagact gaaaataaac agcaaattat
     3241 aaatatgaaa tgttaaaaaa gtatcgataa aaactttatt gttttaagga gataaaatgt
     3301 cgctcgtttg ttctgttata tttattcatc atgccttcaa cgctaacatt ttagataaag
     3361 attacgcctt ctctgacggc gagatcctga tggtagataa cgctgttcgt acgcattttg
     3421 aaccttatga gcggcatttt aaagagatcg gatttactga aaataccatt aaaaaatatc
     3481 tacaatgcac taacatccag acagtgacgg tgcctgttcc tgcgaagttt ttacgtgctt
     3541 caaatgtacc gactggattg cttaatgaaa tgattgctta tctcaactcg gaagaacgca
     3601 atcatcataa tttttcagaa cttttgcttt tttcttgcct gtctattttt gccgcatgca
     3661 aaggtttcat tacactatta actaacggtg tgctatccgt ttccggaaaa gtgagaaata
     3721 ttgtcaacat gaagctggcg cacccatgga agctgaaaga tatttgtgac tgcctgtaca
     3781 tcagtgaaag cctgttgaag aaaaaactaa agcaagagca aacgacattc tcacagattc
     3841 tattagatgc cagaatgcag cacgcaaaaa atttgatacg cgtagaaggt tcagtcaata
     3901 aaattgccga acaatgtggt tatgccagta cttcttattt tatttatgcg ttccgcaaac
     3961 atttcggcaa cagtccgaag agagtttcta aggagtaccg ttgtcaaagt cacacgggta
     4021 tgaatacggg caacacgatg agtgctttag ctatttgatt atttgctaac gagtagtcaa
     4081 ccacgcacgc tacgtaagaa ttatatgggg cagccattcc ctgccccgcg ttgtttttag
     4141 gcgatatatt tattgaaata aataagtgac atccatcaca tatttatgca cttgcataac
     4201 ctgttgcatg attatttatg ttctcaattc agcattttgt cagtaaaatg caataattta
     4261 ttaaatatca ataaattagt tgtttatcgg cgagaaatta cttaatagaa cagaaagtaa
     4321 tgtcaacgct ttatggactg ttatttccct ttttttagct aaatctgcta tctctttatg
     4381 tgactaactt cacttacatc cacttatttc tcttcgtaaa attacttcgg aattaagtac
     4441 aataagaaga ggaacattta tgaagtctgc attaaagaaa agtgtcgtaa gtacctcgat
     4501 atctttgata ctggcatctg gtatggctgc atttgctgct catgcggcag atgatgtaaa
     4561 gctgaaagca accaaaacaa acgttgcttt ctcagacttt acgccgactg aatacagtac
     4621 caaaggaaag ccaaatatta tcgtactaac catggatgat cttggttatg gacaacttcc
     4681 ttttgataag ggatcttttg acccaaaaac aatggaaaat cgtgaagttg tcgataccta
     4741 caaaataggg atagataaag ccattgaagc tgcacaaaaa tcaacgccga cgctcctttc
     4801 attaatggat gaaggcgtac gttttactaa cggctatgtg gcacacggtg tttccggccc
     4861 ctcccgtgcc gcaataatga ccggtcgagc tcccgcccgc tttggtgtct attccaatac
     4921 cgatgctcag gatggtattc cgctaacaga aactttcttg cctgaattat tccagaatca
     4981 tggttattac actgcagcag taggtaaatg gcacttgtca aaaatcagta atgtgccggt
     5041 accggaagat aaacaaacgc gtgactatca tgacaacttc accacatttt ctgcggaaga
     5101 atggcaacct caaaaccgtg gctttgatta ctttatggga ttccacgctg caggaacggc
     5161 atattacaac tccccttcac tgttcaaaaa tcgtgaacgt gtccccgcaa aaggttatat
     5221 cagcgatcag ttaaccgatg aggcaattgg cgttgttgat cgtgccaaaa cacttgacca
     5281 gccttttatg ctttacctgg cttataatgc tccgcacctg ccaaatgata atcctgcacc
     5341 ggatcaatat cagaagcaat ttaataccgg tagtcaaaca gcagataact actacgcttc
     5401 cgtttattct gttgatcagg gtgtaaaacg cattctcgaa caactgaaga aaaacggaca
     5461 gtatgataat actattattc tctttacctc cgataatggt gcggttatcg atggtcctct
     5521 gccgctgaac ggggcgcaaa aaggctataa gagtcagacc tatcctggcg gtactcacac
     5581 cccaatgttt atgtggtgga aaggaaaact tcaacccggt aattatgaca agctgatttc
     5641 cgcaatggat ttctacccga cagctcttga tgcagccgat atcagcattc caaaagacct
     5701 taagctggat ggcgtttcct tgctgccctg gttgcaagat aagaaacaag gcgagccaca
     5761 taaaaatctg acctggataa cctcttattc tcactggttt gacgaggaaa atattccatt
     5821 ctgggataat taccacaaat ttgttcgcca tcagtcagac gattacccgc ataaccccaa
     5881 cactgaggac ttaagccaat tctcttatac ggtgagaaat aacgattatt cgcttgtcta
     5941 tacagtagaa aacaatcagt taggtctcta caatctgacg gatctacagc aaaaagataa
     6001 ccttgccgcc gccaatccgc aggtcgttaa agagatgcaa ggcgtggtaa gagagtttat
     6061 cgacagcagc cagccaccgc ttagcgaggt aaatcaggag aagtttaaca atatcaagaa
     6121 agcactaagc gaagcgaaat aactaaacct tcatgcggcg gaattttccg ccgccttatt
     6181 gagcgagata gcgatgcacg ttacagccaa gccctccagt tttcaatgta atctcaaatg
     6241 tgattactgt ttttaccttg aaaaagagtc gcagtttact catgaaaaat ggatggatga
     6301 cagcactctg aaagagttca tcaaacaata tatcgcagcg tctggcaatc aggtctattt
     6361 tacctggcaa ggcggtgaac ccactctggc tggcctggat tttttccgta aagttattca
     6421 ctatcaacaa cgctatgcag gccaaaaacg tatttttaat gcattacaaa cgaatggcat
     6481 tttattgaat aatgaatggt gttcctttct caaagaacat gaatttctgg ttggtatctc
     6541 gatcgatggc ccccaggagt tacatgaccg ttacagacgc agtaattcag gtaacggtac
     6601 ttttgcaaaa gtgatagcag ccatcgagcg tctgaaatca tatcaagttg agtttaatac
     6661 gttaaccgtc attaataacg ttaatgtcca ttaccctctt gaggtttatc attttttaaa
     6721 atctatcggc agtaaacata tgcaatttat cgaattgctc gaaaccggga cgccgaatat
     6781 tgatttcagt ggtcatagtg agaacacatt ccgtatcatt gatttttctg tgcctcccac
     6841 ggcttatggc aagtttatgt caaccatttt tatgcaatgg gttaaaaacg atgtgggtga
     6901 aattttcatt cgtcagtttg aaagctttgt cagccgtttt ttggggaatg ggcatacaag
     6961 ttgtattttc caggagtcct gcaaggataa tctggttgtt gaaagtaatg gagacattta
     7021 cgaatgcgac cattttgtct atccacagta caaaattgga aacattaata aatctgaact
     7081 caaaacgatg aacagtgtac aactgacagc gcaaaaaaaa cggatttcag cgaaatgtca
     7141 gcaatgtgca tataaaccta tctgcaatgg cggttgtcct aagcatcgta ttactaaagt
     7201 aaacaatgag actgtttctt atttttgcga aggttataaa atcctttttt caaccatggt
     7261 accttatatg aacgccatgg ttgagttagc taagaacaga gtaccgcttt accacattat
     7321 ggatgttgca aaacaaatgg agaataatta ataacgctct cttttaactc cattcttagc
     7381 ctccttctta tgaccttaac aaggcacccg acataaattc ctgtcgggtg tttcgtcatc
     7441 atcatcttcc cttgcgaaaa tcctggctta cgtggtatgc gtcagggcat tagcaatacc
     7501 cttgattatc aatagcataa ccataaccat aaccataacc actgaaaatt aaaaatttct
     7561 atatatttca tattgttaaa tgatacttaa ataaatttca tcagtaaatc attgccatag
     7621 ataatcattg gatggcacaa tcaattttcg ctaatgataa ccattcccat tacgttttgt
     7681 atgttgatag caaagtatct ctgtctgctg aagccctttt ggctgcgtaa gaataataaa
     7741 acttcagtgt tgttaatcat tattatcctc gcaatgatcc ttggcgtggt taaaattcag
     7801 gtctggttaa acgactggaa taatgatttt ttcaatgcgc taagccagaa agaaaccgat
     7861 aagctttggc agcttgttct ttggttccct gccctgctgg ggatctttgt attaatatcc
     7921 gtaaacaaaa catggttaat taaactactg actattcgct ggcgcgaatg gttaacagat
     7981 tactacctta atcgctggtt tgcagataaa aactactact tcacgcagat ctacggcgag
     8041 cataaaaata cagataaccc tgaccaacgt atcgctgaag acattctctt actgatcagc
     8101 aaaacgctca gtctttcatt tggcttcatc cagtcactta gcatgctgat cacctttacc
     8161 gttattcttt ggcaaagcgc gggtacgctc tcattcactg ttggtggaac agaatggaat
     8221 atccaggggt atatggtcta taccgtcgtg ctcattgtga tcggtggaac tttatttacc
     8281 cataaggttg gtaagcgtat tcgtccgctt aatgtggaaa aacaacgtag cgaagcgaca
     8341 tttcgaacta atcttgtgca gcataataag caggcagaac tgattgcgct aagcaatgct
     8401 gagtctctac aacgccagga gttgcgcgac aattttcata ccattaaaga gaactggcat
     8461 cgtttaatga atcgtcagcg gttgcttgat tactggcaaa atatttattc gcgctcgctt
     8521 agcgttcttc cctactttct gttattgccg cagtttatta gcgggcagat caatctgggc
     8581 ggactgatga aatcgcgcca ggcatttatg ctagtatcga acaatttaag ctggtttatt
     8641 tataaatatg acgaacttgc tgaactggct gcggttatcg atcgcttgta tgagttccat
     8701 caactcactg aacagcgccc tacgaataag cctaaaaatt gccaacatgc ggtacaagtg
     8761 gctaatgcga gtattcgtac gcctgataat aagatcatat tagagaactt gaactttcat
     8821 gtttcgccag gcaaatggct attactgaaa ggctactctg gtgcgggaaa aaccacactg
     8881 cttaaaacat tatcccactg ctggccgtgg tttaaaggtg atattttttc tcctgctgac
     8941 agttggtatg tgtcacaaac accgttaatc aaaaccggct tactgaaaga gattatttgt
     9001 aaagcacttc ccctgcccgt agacgataaa tcgttgagcg aagtactgca tcaggttggt
     9061 cttgggaaat tggctgcgcg tattcatgac cacgatcgct ggggagatat tctttccagc
     9121 ggcgaaaaac aacgtatcgc cctggcacga ttaattttac gacgtccgaa atggatattt
     9181 cttgacgaaa ctacctctca tcttgaggaa caagaggcta tccgcttact gcgtttagtg
     9241 cgtgaaaaac tacccacaag cggcgtcatt atggttacac atcaacccgg tgtctggaac
     9301 ctggccgatg atatttgtga cattagcgcg gttttataaa aacaataaaa gccctgctgt
     9361 acggagatat aaataaatga agcgagttct tattcctggc gtcattttat gtggcgctga
     9421 tgtggcgcag gccgtcgatg acaaaaacat gtacatgcat ttttttgaag agatgacggt
     9481 ctatgctcct gtccctgtac ccgtaaacgg caacacgcat tacaccagtg aaagcatcga
     9541 gcgtttaccg accgggaatg gcaatatcag cgatctgctg agaaccaacc ctgcggtacg
     9601 catggattca acgcaaagta cctcgttgaa ccagggagat attcgccctg agaaaatctc
     9661 tattcacggt gcgtcgccct accagaatgc ctatttgatt gacggtatta gtgcaactaa
     9721 taacctgaac ccagcgaatg agtccgatgc cagtagtgca accaatatta gcgggatgtc
     9781 acaggggtat tatcttgatg tcagcttact ggacaatgtg acgctttatg acagttttgt
     9841 gccggttgaa tttggtcgct tcaatggcgg ggtaattgat gcaaagatca aacgcttcaa
     9901 cgctgatgat agcaaggtga aattgggtta tcgcactacg cgttcggact ggttaacatc
     9961 gcatatcgat gagaataaca agagcgcatt taatcaaggt tcttcaggaa gtacttattt
    10021 ctctccagat tttaaaaaga acttttatac cttgtcgttt aatcaggagc tggctgataa
    10081 ctttggcgtt accgccggtt tatcgcgccg ccagtctgat atcacccgcg cggattatgt
    10141 ttcgaatgac ggcattgtcg tcggtcgggc acagtataaa aacgttatcg atactgcatt
    10201 gagcaaattt acctggtttg ccagcgaccg ctttacccac gatttaacct taaaatatac
    10261 cggctccagc cgtgattata ataccagcac cttcccgcag tctgatcgcg aaatgggtaa
    10321 taaatcctat ggtcttgcat gggatatgga tacgcaactc gcatgggcca aactacgtac
    10381 caccgttggt tgggatcata ttagtgatta tacccgtcac gatcatgaca tctggtacac
    10441 cgaactttca tgtacatatg gtgatattac agggcgttgt acccgtggcg gattaggaca
    10501 catttcccag gctgtagata attacacctt caaaacacgc ctggactggc aaaaattcgc
    10561 cgtgggtgat gtttcgcatc aaccctactc cggcgcggaa tacatctatt ccgatgcatg
    10621 gactgaacgc cataaccagt ctgaatccta tgtgattaat gctgcaggaa agaaaactaa
    10681 ccataccatt taccataaag gtaaaggcag cctaggaatt gacaactaca cgctgtatat
    10741 ggcggatcac attagctggc ggaatgtgtc gttaatgccc ggtgtgcgtt atgactatga
    10801 caactatctg tcaaaccaca atatctcccc gcgctttatg acggaatggg atatttttgc
    10861 tgatcaaacc tcaatgatta ccgccggtta taaccgttac tatggcggga atattcttga
    10921 tatgggatta cgtgatatcc gcaatagctg gacggaatcg gtatcaggta ataaaaccct
    10981 gacgcgttat cagaatttga aaacgcctta taacgatgaa ctggcaatgg gattgcagca
    11041 aaaaatcggt aagaacgtta ttgcacgcgc aaactatgtt taccgtgaag cgcatgatca
    11101 aatcagcaaa agcagtcgta ccgacagcgc gactaaaacc accattactg aatataacaa
    11161 cgatggcaaa accaaaacgc attcgtttaa cctcagtttt gaactggccg aacccctgca
    11221 tatccgccag gtagatatta acccgcaaat tgtctttagc tatatcaaga gcaagggcaa
    11281 cttgtcgtta aacaatggtt atgaggagag caataccggt gataaccagg tggtttataa
    11341 cggtaatctg gtctcttacg atagcgttcc agtggcagat tttaataacc cattaaagat
    11401 ctccttaaac atggatttca cgcatcaacc gagcgggtta gtgtgggcga atacgctggc
    11461 gtggcaagaa gcgcgtaaag ctcgcattat ccttggtaag acaaatgcgc aatacatcag
    11521 cgaatattca gattacaagc agtatgttga cgaaaaactg gatagcagcc tgacctggga
    11581 cacccgcttg tcctggacgc cacaatttct gaaacaacaa aacctgacga tcagtgccga
    11641 tattctcaat gtactggata gcaaaaccgc tgttgataca acgaataccg gtgtggcgac
    11701 ctacgccagt ggccgtactt tctggcttga tgtcagcatg aaattttaat agttcggatt
    11761 ttacttaacc gggtaaattc gcccggtttt cgcatggaga taattatgag aaacctctgt
    11821 ttcttactga cgttagtggc aactctgttg ctccccgggc ggctgattgc cgccgcctta
    11881 ccgcaggatg aaaagttaat taccgggcaa ctggacaatg gcttgcgata tatgatttat
    11941 ccgcatgctc aaccaaagga tcaggtaaat ttatggctgc aaattcatac cggttcattg
    12001 caggaagaag acaatgagcg cggcgtggct cattttgtag aacatatgat gtttaacggc
    12061 acaaaaacat ggccgggtaa taaagtcatc gaaacatttg agtcaatggg cctgcgtttt
    12121 ggtcgcgatg ttaatgccta taccagctat gacgaaacgg tgtatcaggt gagtttgccg
    12181 actacgcaga aacaaaatct gcaacaagtg atggcaatct tcagtgaatg gagtaatgcc
    12241 gcaacctttg aaaaactcga agtagacgct gaacgtggcg taattactga ggaatggcgt
    12301 gcccatcagg atgcgaaatg gcgcacctct caggcgcgcc gccctttcct gctggctaat
    12361 acccgtaatt tagaccgtga acctatcggc ctgatggata ctgtcgccac ggtcacaccg
    12421 gcacaattgc gccaatttta tcaacgctgg tatcaaccaa ataatatgac ctttatcgtg
    12481 gtcggcgata tcgacagtaa aaaagcgctg gcgctgataa aggataattt aagtaagctt
    12541 ccggctaaca aagcagctga aaatcgcgtc tggccgacaa aagccgaaaa ccacctgcgc
    12601 tttaatatca ttaatgataa agaaaaccgg gtgaacggca tcgcactcta ttatcgcctg
    12661 ccaatggtac aagtgaacga tgagcaaagc tttatcgaac aagctgaatg gagcatgtta
    12721 gttcagctgt tcaatcaacg tctgcaggaa cgcatacagt cgggcgagtt gaagactatt
    12781 tctggcggca ctgcgcgcag cgttaaaatt gcacccgatt atcagtcgct gtttttccgt
    12841 gtaaatgcac gagacgataa tatgcaggat gctgcgaatg cattaatggc agagttggca
    12901 accattgatc agcatggttt ttctgctgaa gaactcgatg atgtcaaatc tacccgcctc
    12961 acctggctga aaaatgcggt tgatcagcaa gctgaacgtg atttacgtat gctgaccagt
    13021 cgcctggcat ccagctcatt aaataatacg ccgttcttgt cgccggaaga gacatatcaa
    13081 ctttcgaaac gtctgtggca gcaaattacc gtgcaaagtc tggcggaaaa atggcagcag
    13141 ttaagaaaga accaggacgc attttgggag caaatggtaa acaatgagct tgccgccaaa
    13201 aaagcattgt ctcctgcggc tatcctggcg ctggaaaaag agtacgccaa caaaaagctg
    13261 gcggcttaca tcttcccagg cagaaattta tcgttaacag tagacgctga cccacaggcg
    13321 gaaattagca gcaaagaaac gctggctgag aatctgacat cattaacact ttccaatggt
    13381 gccagggtta ttctggcaaa atccgcgggt gaagagcaaa agctacaaat tactgccgta
    13441 tcgaataaag gcgatttaag tttccctgcg cagcaaaaat cacttatcgc gctggcaaat
    13501 aaagcggtaa gcggaagcgg cgttggtgag ctctcctctt ccagcctgaa acgctggagt
    13561 gcggaaaatt cggtaaccat gagcagtaaa gtcagtggca tgaatacgtt gctctccgtt
    13621 agcgcgcgga ctaataaccc tgaacctggt tttcagttga ttaaccagcg aatcacccac
    13681 agcacgatta acgacaatat ttgggcatcg ctacaaaatg ctcaaattca ggcgttgaaa
    13741 acgctcgacc agcgtccagc ggagaaattc gcccagcaga tgtatgagac gcgctatgct
    13801 gatgaccgca cgaaattact gcaagaaaat cagattgtac agtttactgc cgcagatgcg
    13861 ctggctgccg atcgccagtt gttttcatct ccagcggata tcacgtttgt cattgtcggt
    13921 aatgtctcag aagacaaact cgtggcgtta attacgcgtt acttaggatc aatcaaacac
    13981 tctgattcgc cattagccgc aggtaaacca ttaactcgcg cgacggacaa cgcatcggtt
    14041 actgtaaaag aacaaaatga acctgtggca caggtttcac agtggaagcg ttatgattcc
    14101 cggacacctg ttaatctggc gacgcgtatg gcgctcgatg cttttaacgt cgcactggca
    14161 aaagatctac gtgttaatat tcgtgaacag gcatctggag catacagcgt ttcttctcgc
    14221 ctctcggttg atcctcaggc caaagatatc agtcatttgc tggcttttac ttgtcaacca
    14281 gaacgacatg atgaactgtt aacgttagcg aatgaagtga tggttaagcg tctggctaaa
    14341 gggatcagtg agcaagaact gaatgaatac cagcaaaacg ttcagcgcag cctcgatatc
    14401 caacagcgta gcgttcaaca attagcgaac actattgtaa atagtcttat tcaatatgac
    14461 gatcctgcag catggactga gcaggagcaa ttgttgaaac aaatgacggt agagaatgtt
    14521 aacactgccg ttaaacaata tctttctcat ccggtaaata cttataccgg agtattattg
    14581 ccaaaataat aacagccccg tcaacacatc gttggcgggg attttagcaa tattcgctat
    14641 ttttatgtaa taattttata aatgcgttca aaataataat caagtactaa tagtgatatt
    14701 ttaaggtctg atttttacgt gataattcag gagacacaga atgcgcataa aaataacagc
    14761 ataaaacacc ttaccaccac ccaagaattt catattgtat tgtttttcaa tgaaaaaaca
    14821 ttattcgcgt aatatctcac gataaataac attaggattt tgttatttaa acacgagtcc
    14881 tttgcacttg cttactttat cgataaatcc tactttttta atgcgatcca atcattttaa
    14941 ggagtttaaa atggataaga agcaagtaac ggatttaagg tcggaactac tcgattcacg
    15001 ttttggtgcg aagtctattt ccactatcgc agaatcaaaa cgttttccgc tgcacgaaat
    15061 gcgcgacgat gtcgcattcc agattatcaa tga
//
LOCUS       DADLFA010000097        14935 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000097 DADLFA010000000
VERSION     DADLFA010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14935
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.097"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..450
                     /locus_tag="JQH65_002503"
     CDS             <1..450
                     /locus_tag="JQH65_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HAY6139884.1"
                     /translation="QEVNAALFKEYLPLLQQSEPTIKQPVRWKNALGELNANLDISIA
                     DPAKSSSSTNKDIKSLNFDVKLPLNVVTETAKQLNLSEGMDAEKAQKRADKQISGMMT
                     LGQMFQLITIDNNTASLQLRYTPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(491..1423)
                     /locus_tag="JQH65_002504"
     CDS             complement(491..1423)
                     /locus_tag="JQH65_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY6139885.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
     gene            complement(1543..1785)
                     /locus_tag="JQH65_002505"
     CDS             complement(1543..1785)
                     /locus_tag="JQH65_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6139886.1"
                     /translation="MSVPVYQIMGKGCHRQDKNMSFSPLFVKIFCRSSGSLANRHVDN
                     FVHKLFTSLLIYYHAFYSMMFVFVFHVVKNKAIPNN"
     gene            1787..4573
                     /gene="polA"
                     /locus_tag="JQH65_002506"
     CDS             1787..4573
                     /gene="polA"
                     /locus_tag="JQH65_002506"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAY6139887.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLQQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNPLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            complement(4954..5586)
                     /locus_tag="JQH65_002507"
     CDS             complement(4954..5586)
                     /locus_tag="JQH65_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAY6139888.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRHSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            6168..6677
                     /gene="yihI"
                     /locus_tag="JQH65_002508"
     CDS             6168..6677
                     /gene="yihI"
                     /locus_tag="JQH65_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HAY6139889.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            6866..8239
                     /gene="hemN"
                     /locus_tag="JQH65_002509"
     CDS             6866..8239
                     /gene="hemN"
                     /locus_tag="JQH65_002509"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAY6139890.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(8429..8539)
                     /gene="yshB"
                     /locus_tag="JQH65_002510"
     CDS             complement(8429..8539)
                     /gene="yshB"
                     /locus_tag="JQH65_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HAY6139891.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(8650..10059)
                     /gene="glnG"
                     /locus_tag="JQH65_002511"
     CDS             complement(8650..10059)
                     /gene="glnG"
                     /locus_tag="JQH65_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HAY6139892.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            complement(10071..11120)
                     /gene="glnL"
                     /locus_tag="JQH65_002512"
     CDS             complement(10071..11120)
                     /gene="glnL"
                     /locus_tag="JQH65_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HAY6139893.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLEIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            complement(11294..12703)
                     /gene="glnA"
                     /locus_tag="JQH65_002513"
     CDS             complement(11294..12703)
                     /gene="glnA"
                     /locus_tag="JQH65_002513"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HAY6139894.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            13076..14899
                     /gene="typA"
                     /locus_tag="JQH65_002514"
     CDS             13076..14899
                     /gene="typA"
                     /locus_tag="JQH65_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HAY6139895.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDY"
BASE COUNT         3862 a         3746 c         3851 g         3476 t
ORIGIN      
        1 caagaagtga atgctgcatt gttcaaagag tatttaccgt tattacaaca aagtgagccg
       61 accattaaac aaccggtaag atggaagaac gcactcggcg aactaaatgc caatctggat
      121 atcagtattg ccgacccagc caaatcttca tcatccacaa acaaagatat caaatcgctc
      181 aattttgatg tgaagttacc gcttaatgtc gtcacagaaa ccgcaaaaca gcttaattta
      241 tctgaaggaa tggatgcgga aaaagcgcaa aagcgggctg ataaacaaat cagcgggatg
      301 atgaccttag gtcagatgtt tcagttaatc acgattgaca acaataccgc ctcgctgcaa
      361 ctgcgttata caccgggtaa agttgttttt aacggacagg agatgagcga agaagaattt
      421 atgtctcgtg ccggacgttt tgttcattaa aatactcact cgcctctgct cattcagagg
      481 cgagcaaaat ttacttgtcc tgccgctgtg atgacatcaa cgacgtcaga agtcggtctt
      541 tttcctgcca cagtgagttc agccattgct gaaaatggcg cttaaagctt ttgtcattga
      601 tgtagtcgcc atgtaactca tcggcaatag gctgtaaatc cacatgaacg acaattcgcg
      661 tcagtttacc gcttaacata tcgaagaacg gctgacggtt attgtccgga taacacagcg
      721 taacattaag cagtttatcg aattgtttac ccagtacatt tagcgccatc gcaatgcctg
      781 ccgcctttgg tggcaacaag ttttgaaaag tggagtgggt ttgctgatgt ttttcttgcg
      841 tgaaacggga gccttcaacg aaattgacaa tggtggtggg atgcaggcga aacttttcac
      901 aagaacgccg agtggtttca acatctttac cacgtcgctc cggatgacgt aataaataag
      961 cgcgggaata acgcttcata aacggcatat ccagcgccca gcacgccagg ccaagaaatg
     1021 gcacccaggc cagctgttgc ttgaggaaat atttattcat cggaatgtgc ttacgaaaca
     1081 gcacgcacag tacgacaata tctgcccaac tacggtggtt acaaataagc agataccagt
     1141 tcttcttact taacccttcc agcccgtgaa cttcccattg caggtgtggg ttcagatgca
     1201 gtaataccgc cagaccttca caccagcaat acatcataaa atcacaaaaa cgcgaaacct
     1261 ttcgccaaat gactggcaca ggtagcaata gttttacaat cccggcaatg atgatcggga
     1321 cggaacaaaa aatagtgacc aaaatggtca atacgatact cagcaaaagg gttatcgcag
     1381 cgagtattct cgtcataatg aatttattca aaaggttagc catagacagt gcgctaaaga
     1441 agcgcaaggc gcagatttta gcagaaaacg acccaaataa cggatgatcc ttaaggagaa
     1501 aaataattca tatctatcca cattagaaaa aatcccatta tctcaattat tagggatggc
     1561 tttattttta actacatgaa aaacaaagac aaacatcata ctgtaaaaag catgataata
     1621 aattaaaagc gatgtaaata atttatgcac aaagttatcc acatgacgat ttgcgagcga
     1681 tccagaagat ctacaaaaga ttttcacgaa aagcggtgaa aaactcatgt ttttatcctg
     1741 tctgtggcat cctttaccca taatctgata aacaggcacg gacattatgg ttcagatccc
     1801 ccaaaatcca cttatccttg tagatggttc atcttatctt tatcgcgcat atcacgcgtt
     1861 tcccccgctg actaacagcg caggcgagcc gaccggtgcg atgtatggtg tcctcaacat
     1921 gctgcgcagt ctgatcatgc aatataaacc gacgcatgca gcggtggtct ttgacgccaa
     1981 gggaaaaacc tttcgtgatg aactgtttga acattacaaa tcacatcgcc cgccaatgcc
     2041 ggacgatctg cgagcacaaa tcgaacctct gcacgcgatg gttaaagcga tgggactgcc
     2101 gctgctggcg gtttctggcg tagaagcgga cgacgttatc ggtactctgg cgcgcgaagc
     2161 cgaaaaagcc gggcgtccgg tgctgatcag cactggcgat aaagatatgg cgcagctggt
     2221 gactccaaat attacgctta tcaataccat gacgaatacc atcctcggac cggaagaggt
     2281 ggtgaataag tacggcgtgc cgccagaact gatcatcgat ttcctggcgc tgatgggtga
     2341 ctcctccgat aacatccctg gcgtaccggg cgtcggtgaa aaaaccgcgc aggcattgct
     2401 gcaaggtctg ggcgggctgg atacgctgta tgccgagcca gaaaaaattg ctgggttgag
     2461 cttccgtggc gcgaaaacaa tggcagcgaa gctccagcaa aacaaagaag ttgcttatct
     2521 ctcataccag ctggcgacga ttaaaaccga cgttgaactg gagctgacct gtgaacaact
     2581 ggaagtgcag caaccggcag cggaagagtt gttggggctg ttcaaaaagt atgagttcaa
     2641 acgctggact gctgatgtcg aagcgggcaa atggttacag gccaaagggg caaaaccagc
     2701 cgcgaagccg caggaaacca gtgttgcaga cgaagcgcca gaagtgacgg caacggtgat
     2761 ttcttatgac aactacgtca ccatccttga tgaagaaaca ctgaaagcgt ggattgcgaa
     2821 gctggaaaaa gcgccggtat ttgcatttga taccgaaacc gacagccttg ataacatctc
     2881 tgctaacctg gtcgggcttt cttttgctat cgagccaggc gtagcggcat atattccggt
     2941 tgctcatgat tatcttgatg cgcccgatca aatctctcgc gagcgtgcac tcgagttgct
     3001 aaaaccgctg ctggaagatg aaaaggcgct gaaggtcggg caaaacctga aatacgatcg
     3061 cggtattctg gcgaattacg gcattgagct gcgtgggatt gcgtttgata ccatgctgga
     3121 gtcctacatt ctcaatagcg ttgccgggcg tcacgatatg gacagcctcg cggaacgttg
     3181 gttgaagcac aaaaccatca cttttgaaga gattgcgggt aaaggcaaaa atccactgac
     3241 ctttaaccag attgccctcg aagaggccgg acgttacgcc gccgaagatg ctgatgtcac
     3301 cttgcagttg catctgaaaa tgtggccgga cctgcaaaaa cacaaagggc cgttgaacgt
     3361 cttcgagaat atcgaaatgc cgctggtgcc ggtgctttca cgcattgaac gtaacggtgt
     3421 gaagatcgat ccgaaagtgc tgcacaatca ttctgaagag ctcaccttgc gtctggctga
     3481 gctggaaaag aaagcgcatg aaattgcagg tgaagaattt aacctttctt ccaccaagca
     3541 gttgcaaacc attctgtttg aaaaacaggg tattaaaccg ctgaagaaaa cgccgggtgg
     3601 cgcgccgtca acgtcggaag aggtactgga agaactggcg ctggactatc cgttgccaaa
     3661 agtgattctg gagtatcgtg gtctggcgaa gctgaaatcg acctacaccg acaagctgcc
     3721 gctgatgatc aacccgaaaa ccgggcgtgt gcatacctct tatcaccagg cagtaactgc
     3781 aacgggacgt ttatcgtcaa ccgatcctaa cctgcaaaac attccggtgc gtaacgaaga
     3841 aggtcgtcgt atccgccagg cgtttattgc gccagaggat tatgtgattg tctcagcgga
     3901 ctactcgcag attgaactgc gcattatggc gcatctttcg cgtgacaaag gcttgctgac
     3961 cgcattcgcg gaaggaaaag atatccaccg ggcaacggcg gcagaagtgt ttggtttgcc
     4021 actggaaaca gtcaccagcg agcaacgccg tagcgcgaaa gcgatcaact ttggtctgat
     4081 ttatggcatg agtgctttcg gtctggcgcg gcaattgaac attccacgta aagaagcgca
     4141 gaagtacatg gacctttact tcgaacgcta ccctggcgtg ctggagtata tggaacgcac
     4201 ccgtgctcag gcgaaagagc agggctacgt tgaaacgctg gacggacgcc gtctgtatct
     4261 gccggatatc aaatccagca atggtgctcg tcgtgcagcg gctgaacgtg cagccattaa
     4321 cgcgccaatg cagggaaccg ccgccgacat tatcaaacgg gcgatgattg ccgttgatgc
     4381 gtggttacag gctgagcaac cgcgtgtacg tatgatcatg caggtacacg atgaactggt
     4441 atttgaagtt cataaagatg atgttgatgc cgtcgcgaag cagattcatc aactgatgga
     4501 aaactgtacc cgtctggatg tgccgttgct ggtggaagtg gggagtggcg aaaactggga
     4561 tcaggcgcac taagattcgc ctgaacatgc ccttttttcg taagtaagca acataagctg
     4621 tcacgttttg tgatggctat tagaaattcc tatgcaacaa ctgaaaaaaa ttacaaaaag
     4681 tgctttctga actgaacaaa aaagagtaaa gttagtcgcg tagggtacag aggtaagatg
     4741 ttctatcttt cagacctttt acttcacgta atcggatttg gctgaatatt ttagccgccc
     4801 cagtcagtaa tgactggggc gttttttatt gggcgaaaga aaagatccgt aatgcctgat
     4861 gcgctatgtt tatcaggcca acggtagaat tgtaatctat tgaatttacg ggccggatac
     4921 gccacatccg gcacaagcat taaggcaaga aaattattcg ccgtcctgcg tttcttctac
     4981 aggctgcatc tcgctaaacc aggtatccag tttctgccgc agcttgtcca cgccttgttt
     5041 cttcaacgaa gaaaacgttt caacctgcac atcaccgtta aacgccagta cagcttcacg
     5101 caccatattc aattgcgctt tacgtgcgcc gcttgccagt ttgtccgctt tggtcagcag
     5161 caccagaacg gcgatattgc tgtctaccgc ccactcaatc atctgctgat ccaaatcttt
     5221 cagcggatgg cgaatatcca ttagcaccac cagaccttgc aggctatgac gtttttcgag
     5281 gtattcgccg agcgcacgct gccatttgcg cttcatctct tccgggactt ccgcataacc
     5341 gtacccaggc aagtcaacca gacgcttgcc gtcagccact tcaaacaggt tgataagctg
     5401 ggtgcgccct ggggtttttg aggtacgagc caggcttttc tggttagtca gcgtgttcag
     5461 cgcgctggat ttacctgcgt tggaacggcc tgcaaaagcc acttcaattc cggtatcgga
     5521 aggtaggtgg cgaatatcag gcgcactcat cacaaaatgc gtctgttgat aattcaaatt
     5581 agtcaaagcg gtcgtctccg tcagtcaaag ctttgtggcg attatacctg aaccataata
     5641 aaaggcggat ttttcggcgt gagcgttgta agtaaaagcc atacgctttg tgagacattg
     5701 ccgatagtct ttatgcgaaa tagcagagaa aattctgcga tgcatgtcaa ataagctata
     5761 taaatcagtg aattgctttt atataaaact ctgaaaatgt gcaaatactg atggcggttg
     5821 attgtttgtt taaagcgaag gcgtaaagta gcacccatag agcgaggacg ctaacaggaa
     5881 caatgactca ggatgagggt caggagcgcc aggaggcgaa gacagaggat tgtcaggaag
     5941 acaaacgtcc ggagacgtaa ttaaacggaa atggaatcaa cacggattgt tccggctaaa
     6001 ggaaaaacag ggtgtgttgg cggcctgcaa ggattgtaag acccgttaag ggttatgagt
     6061 caggaaaaaa ggcgacagag taatctgtcg ccttttttct ttgcttgctt tctgttagat
     6121 tccgccgcaa atcgatactg aataaaacgg ctaaaagacg aaccattatg aaaccatcat
     6181 cttcaaactc acgcagcaaa ggtcatgcaa aagcgcgtcg aaaaacacgc gaggagctgg
     6241 atcaggaagc tcgtgatcgc aagcgtcaga aaaaacgtcg tggtcatgcg ccgggcagcc
     6301 gcgcagcggg cggaaacacc acgtcaggca gcaaaggcca gaacgcacca aaagatccac
     6361 gtattggcag taaaacccct attccattgg gcgtgactga aaaagtcacc aaacagcaca
     6421 aaccgaagag tgagaaacct atgctttcac cgcaggcgga gttggaatta ctggaaacgg
     6481 atgagcgtct ggatgcgctg ctggaacgtc tggaagcagg cgaaaccctg agtgccgaag
     6541 agcaatcctg ggtggatgct aaactggatc gtattgatga gttgatgcag aaactcggcc
     6601 tctcttatga cgatgacgaa gaagaggaag aagacgagaa gcaagaagac atgatgcgtc
     6661 tgctgcgggg caactaacgg attgccgccg tgggccttcc cgttctgctg ataacccttc
     6721 cggttatatg ttatctggtg tggttattcg ttaaactaca gcggttgtcg cggcgacaaa
     6781 agtggctgcg caaccggctg atgacccgaa acgggcatcc gccggtacgc cgtagccgcc
     6841 agagacgcca tcggaaggag tgagcatgtc tgtacagcaa atcgactggg atctggccct
     6901 gatccagaaa tataactatt ccgggccacg atacacctcg tacccgaccg cgctggagtt
     6961 ttcagaagac ttcggcgaac aggcgttttt acaagccgtg gcgcgctatc ctgagcgtcc
     7021 attatctctc tacgtacata tcccgttctg ccataagctt tgttacttct gcggttgcaa
     7081 taagattgtt actcgccagc agcacaaggc cgatcagtat ctggacgcgc tggagcaaga
     7141 aatcgtccat cgtgcaccgc tgttcgccgg gcgtcacgtc agccaattgc actggggcgg
     7201 cggaacgccg acgtatctga ataaagcgca aatcagccgc ctgatgaagc tgctgcgcga
     7261 aaacttccag ttcaatgccg atgcggagat ttcgatcgaa gtcgatccgc gggaaatcga
     7321 actggatgta ctcgatcatt tacgcgccga aggctttaat cgcctgagca tgggcgtgca
     7381 ggacttcaac aaagaagtgc aacgtctggt taaccgcgag caggatgaag agttcatctt
     7441 tgcactgctt aaccatgcgc gtgagattgg ttttacctcc accaacatcg acctgattta
     7501 cggcctgccg aaacagacgc cggagagttt cgcctttacc ctgaaacgtg tggcggagct
     7561 gaaccccgat cgtctgagtg tctttaacta cgcgcatctg ccgaccattt ttgctgctca
     7621 gcgcaaaatc aaagatgctg acctgccgag tccgcagcaa aaactcgata tcctgcagga
     7681 aaccatcgcc ttcctgacgc aatcgggcta tcagtttatc ggtatggatc actttgcccg
     7741 tccggatgac gagctggcgg tggcccagcg tgaaggcgtg ctgcatcgta acttccaggg
     7801 ctacaccact cagggcgata ccgatctgct ggggatgggc gtttccgcca tcagcatgat
     7861 tggcgactgc tacgcgcaga accagaaaga gttgaagcag tactatcagc aagtggatga
     7921 acaaggcaat gcgctgtggc gtggtattgc gctaacgcgt gatgactgta ttcgccgcga
     7981 tgtgattaag tcgctcatct gcaacttccg tctggattac gcccctattg agaaacagtg
     8041 ggatttgcac ttcgctgatt actttgcgga agatctcaag ctgctcgccc cgttagcaaa
     8101 agatgggctg gtggatgtgg atgagaaggg aatacaggtg acggcgaaag gtcgcttgct
     8161 gatccgcaac atttgcatgt gctttgatac ctatctgcgc cagaaagcgc ggatgcagca
     8221 gttctcacgg gtgatttgaa tagtgaatgg cgcttcgttt acaaagtaga aaacgaaagc
     8281 gccatcaatg tgctgagaac aagattgcct gatgcgctac gcttatcagg cctacaaaac
     8341 ctgttgaaaa tattgctttt gtaggccgga taaggcgttc acgccgcatc cggcatgaac
     8401 aaagagcact tcatcagcaa tctgaaactc aactaaacag cccaatcatt gcggcacaca
     8461 gcacggcagc aacggcagtt tgcggtgtgt ggctgtcaac tgcgccactc gataccagat
     8521 taattattga ttccagcata atgactctcc ccgttttccg ggcagatcat actgaactta
     8581 tcggaacagt aaagcgtaaa ataccagcaa tttgcgctca ataatcaatc tttacacaca
     8641 agctgtgaat cactccatcc ccagctcttt taacttacgc gtcagggtgt tgcggcccca
     8701 gccgagtagc cgcgccgctt cctgtttatg cccctgcgta tgtcgcaacg cggtcgtcag
     8761 taacgtccgc tccagctctg gctgcgcttc ggaaagcaga ttttgatgac cggaacgcag
     8821 cgctctgtct gcccactgcg caagaagcgt cgcccagctg tccggttgca tttgcgaagt
     8881 actctccgca accgttgatt caaacagttc gccgggcaaa tcctgaatca acacttcctg
     8941 cccggcggcc atcaccgtta gccagcggca ggtgttttcc agctggcgca cgttgcctgg
     9001 ccacgccaga cgcgtcagag cagcttcggt ttccggatgc agtaacttcg cttctacgcc
     9061 cagttcgcgc gcggcaacct gtaaaaaatg gcgcgccaga cggggaatat cttcccgacg
     9121 ttcgcgcagc ggcggtagat gaacgcggat aacgtttagg cggtggaaca gatcctcacg
     9181 gaacttacct tcctgcactc gctgttcgag attctggtga gtggcagcga taatccgcac
     9241 atccactttc accggcgcat agccgccaac gcggtaaaac tgaccgtctg ccagcacgcg
     9301 cagcaaacgc gtctgcacat ccagcggcat atcaccaatt tcgtcgagga ataatgtacc
     9361 gccatcggcc tgttcaaaac gcccctgacg aatggtattc gcgccagtaa acgcgccttt
     9421 ctcgtggcca aacagttctg attcgatcaa atcttttggg atagctgcca tattcagcgc
     9481 gataaacggt gctttggcgc gcggactgtg gcgatgcagg gcatgagcga ccagttcttt
     9541 accggtgccg gattcgccgt taatcagcac gctaatagaa gaacgcgaaa gccgaccgat
     9601 aatacggaac acgtcctgca tggctggcgc ttcgccgatg atatcggtcg ttgggccgtt
     9661 aagctgaata ttacgcggct gctgctgttc ctggtaatga ctgatagcgc gctcaaccag
     9721 cgccacggct tcgtcgatat caaacggttt gggcagataa tcaaacgccc cttgttgata
     9781 ggcgctgacg gcagcatcca gatcggaatg tgcggtcata atgatgaccg gaagcattgg
     9841 atggcgctgt ttaatctgct tgagcagcgc cagcccgtcc attcccggca tacggatatc
     9901 tgaaagcagc acatccggcg ttttgctcgc cagcgcctcc agtacttccg cgccgttctc
     9961 aaatgtcgta caggttaaac ccgctccagc gagcgcacgt tcaagcaccc aacggatgga
    10021 actatcgtca tcgactaccc agactatccc tcgttgcata aacgtcacct ttatttcctg
    10081 ataggcaggt aaaccgagaa ctcggtatga cctggccaac tggtaaattc aattttgcct
    10141 gaatgctgat caatcaaatt acgggcgatg gataagccaa gcccggtgcc accttcgcgg
    10201 ccgctgacca tcgggtaaaa cagcgtatcc tgcaaatgag gcggaatgcc cggcccgtta
    10261 tcttccacat caatccgcgc cgccagccgg tagcgctcgc cgtgtaaggt cagttgaaac
    10321 gcggtgcggg tacgcagaat gatttcaccg ccttccggcc ccagcgcctg tagcgcattg
    10381 cgcacaatat tcagcaagac ctgttcaatt tgatccgggt cgtgcgccag ttccggcagg
    10441 ctgggatcgt aatcacgaat caaccgcacg ttgtccggca gttccatcga caccagcgtt
    10501 accacgcgtt cagccacttt gtgaatactt tcggtaacgc gcgtaccggg cagctgcggc
    10561 cccaacagac ggtcgaccag atttcgcagc cggtccgcct gttcgataat cactttggta
    10621 tattcgagta gtgatgggtc aggtaacgct ttgctgagca gctgcgccgc gccacgtaaa
    10681 ccgccaagcg gatttttaat ctcatgtgcc aggccgcgca ctaaatcacg ggcagcaacc
    10741 tgctgggcgt gctgtagctg ttcctgactt aagcggcgct ggttatccat cggagccatc
    10801 tccagcagga tcatgccgtc cggcatacgc tgggccgtca cagaaaggat atgcgagcgc
    10861 ccgtcgatga ccagcgtcac ttcgttatcg gtaaaacctt gccccgcctc cagactttct
    10921 tgcatcagct cgatatttaa tgagaagtag ctcaacagtt ccggtaacgg tgtaccaaac
    10981 aatttgcggg agctttgggc gagcagttgt tgcgcggcag ggttggcgta atggatctcc
    11041 aggttgtcat cgattaacaa aatactgtta atcagcgagt tgaggatctg cccagcatcg
    11101 ggctgcgtgc ctgttgccat aaagcagtct cctgaacagg ttgcaccatt ttagtgcatt
    11161 atagcttttt acggataaaa agcgcgaagc atcagagaat tgacggagaa aaaagcccat
    11221 gcagagatgg gctgaaagtt tccacggcaa ctaaatcccg gcgttgttgc gccgggtagt
    11281 acaacttcaa ctattagacg ctgtagtaca gctcaaactc taccggatgc ggagtcatac
    11341 gcacgcggtc atcttcttcg cgacgcagag cgatgtacgc atcaattgct tcgtcagtga
    11401 acacgccacc ggctttcagg aactcgcggt ccagatccag ttcgttcagt gcttcttcca
    11461 gagagcctgc aacctgtggg atctctttcg cttcttctgg cggcaggtca tacaggtttt
    11521 tgtccatggc ttcacccgga tggatcttgt tcttgatacc atcaagaccg gccatcagca
    11581 gggcagcaaa gcacaggtac gggttagctg ccggatccgg gaaacgtact tcgatacgac
    11641 gtgctttcgg agaagaaacc accggaatac ggatagacgc agaacggtta cgcgcagagt
    11701 aagccagcat taccggtgct tcatagcccg ggaccagacg cttataagag ttggtggtcg
    11761 ggtttgccag ggcgttaatc gctttagcgt gtttgattac gccgccaatg tagtacagcg
    11821 cctgctcaga cagacctgcg tatttgtcgc ctgcgaacag gttaacgccg tttttagaca
    11881 gagacatgtg gcagtgcata ccggagccgt tatcaccgaa catcggtttt ggcataaagg
    11941 tcgcggtttt accgaagcgg tgcgctacgt tgtgcacaac atatttgtag atctgaattt
    12001 cgtcagcttt tttggtcatg gtattgaagc gggtagccac ttcgttctga ccagcagtcg
    12061 ctacttcgtg gtgatgggct tcaaccacca gccccatctg ttccatcacc agacacattt
    12121 cagaacgaat atcctgagcc gagtctaccg gtggaaccgg gaagtaaccg cctttcactg
    12181 ccggacggtg acctttgtta ccaccttcgt attgggtgga ggagttccat gcgccttcga
    12241 tatcgtcgat agcaacgtgg gaaccggaga tagatgatcc gaaacggatg tcatcgaaca
    12301 ggaagaattc aggttctggc ccgaacagta cggtgtcggc aatgccagtg gaacgcaggt
    12361 aatcttcggc gcgcttcgca atggagcgcg ggtcacggtc atagccttgc agggtgccag
    12421 gttcaaggat gtcgcaacgg ataatcaggg tggagtcggc gaagaacggg tcaatcactg
    12481 cggtggatgc gtctggcatc agcaccatgt cggactcgtt aatgcctttc cagccgccaa
    12541 tcgaggagcc gtcaaacatt ttgccttctt cgaagaattc agcattcacc tgatgagcag
    12601 ggatagtgac gtgctgttct ttacctttag tatcggtgaa gcgcaaatca acaaacttca
    12661 cttcgtgctc gttcagcatc gtcagtacgt gttcagcgga catactttaa ctctcctgga
    12721 ttggtcatgg tcgtcgtggt aacgaaatct gcaattattt tggccgtgtc gccgtaaaaa
    12781 agataaagcg aaatctgtgc caacttttaa attgccccta aaaggcgtta tcatgcgcac
    12841 catcgtgcaa aagggctgca ccacgatgtg aatgttgcac caatatagtg cttcaatgga
    12901 aacattaagc accatgttgg tgcaatgacc tttggataac cctttttatg ctccgtgaaa
    12961 gcgatcacaa agggactctg caatacttgt ttgcggagga tgtttgtgat cctgttttgt
    13021 agtgcgatta atccgtgtac aataacgcgc tatttctaat gcctgaggca aagttgtgat
    13081 cgaaaaattg cgtaatatcg ccatcatcgc gcacgtagac catggtaaaa ccaccctggt
    13141 agacaagctg ctccaacaat ccggtacgtt cgactctcgt gccgaaaccc aagagcgcgt
    13201 gatggactcc aacgatttgg agaaagagcg tgggattacc atcctcgcga aaaacaccgc
    13261 tatcaaatgg aatgattacc gtatcaacat cgttgatacc ccggggcacg ccgacttcgg
    13321 tggtgaagtt gaacgtgtaa tgtccatggt agactcagtg ctgctggtgg ttgacgcatt
    13381 tgacggcccg atgccgcaaa cgcgcttcgt aaccaaaaaa gcgtttgctt acggcctgaa
    13441 gccgattgtt gttatcaaca aagttgaccg ccctggcgcg cgtcctgatt gggttgtgga
    13501 tcaggtattc gatctgttcg ttaacctcga cgcgaccgac gagcagctgg acttcccgat
    13561 cgtttacgct tctgcgctga acggtatcgc gggtctggac cacgaagata tggcggaaga
    13621 catgaccccg ctgtaccagg cgattgttga ccacgttcct gcgccggacg ttgaccttga
    13681 cggtccgttc cagatgcaga tttctcagct cgattacaac agctatgttg gcgttatcgg
    13741 cattggccgc atcaagcgcg gtaaagtgaa gccgaaccag caggtcacta tcatcgatag
    13801 cgaaggcaaa acccgcaacg cgaaagtcgg taaagtgctg ggccacctcg gtctggaacg
    13861 tatcgaaacc gacctggcgg aagctggcga tatcgttgcg atcacgggcc ttggcgaact
    13921 gaacatttct gacaccgttt gcgacacgca aaacgttgaa gcgctgccgg cactctccgt
    13981 tgatgagccg accgtttcta tgttcttctg cgttaacacc tcgccgttct gcggtaaaga
    14041 aggtaagttc gtaacgtctc gtcagatcct ggatcgtctg aacaaagaac tggtacacaa
    14101 cgttgcgctg cgcgtagaag aaaccgaaga cgctgatgcg ttccgcgttt ctggtcgtgg
    14161 cgaactacac ctgtctgttc tgatcgaaaa catgcgtcgt gaaggtttcg aactggcggt
    14221 atcccgtccg aaagttatct tccgtgaaat cgacggtcgt aaacaagagc catatgaaaa
    14281 cgtgactctg gacgttgaag aacagcatca gggttctgta atgcaggcgc tgggcgaacg
    14341 taaaggcgac ctgaaaaaca tgaatccaga cggtaaaggc cgcgtacgtc tcgactacgt
    14401 gatcccaagc cgtggtctga ttggcttccg ttctgagttc atgaccatga cttccggtac
    14461 tggtctgctg tactccacct tcagccacta cgacgacgta cgtccgggtg aagtgggtca
    14521 gcgtcagaac ggcgtactga tctctaacgg tcagggtaaa gcggtcgcgt tcgcgctgtt
    14581 cggtctgcag gatcgcggta agctgttcct cggtcacggt gcagaagttt acgaaggtca
    14641 gattatcggt attcatagcc gctctaacga cctgactgta aactgcctga ccggtaagaa
    14701 actgaccaac atgcgtgctt ccggtactga cgaagccgtt gttctggttc cgcctatccg
    14761 catgactctg gaacaagctc tggagttcat cgatgatgac gaactggtag aagtgactcc
    14821 gacctctatc cgtattcgta aacgtcacct gacggaaaac gatcgtcgtc gcgccaaccg
    14881 cgcaccgaaa gactattaat ttcgtttttt agttataaaa aacctgccat cgctg
//
LOCUS       DADLFA010000098        14560 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000098 DADLFA010000000
VERSION     DADLFA010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14560
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.098"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..209)
                     /locus_tag="JQH65_002515"
     CDS             complement(<1..209)
                     /locus_tag="JQH65_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAY6139896.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="JQH65_002516"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="JQH65_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAY6139897.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="JQH65_002517"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="JQH65_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006714698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAY6139898.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="JQH65_002518"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="JQH65_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003852912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAY6139899.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="JQH65_002519"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="JQH65_002519"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HAY6139900.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="JQH65_002520"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="JQH65_002520"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HAY6139901.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="JQH65_002521"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="JQH65_002521"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HAY6139902.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYRGGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="JQH65_002522"
     CDS             complement(4598..5320)
                     /locus_tag="JQH65_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY6139903.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5488..6300)
                     /gene="fkpA"
                     /locus_tag="JQH65_002523"
     CDS             complement(5488..6300)
                     /gene="fkpA"
                     /locus_tag="JQH65_002523"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003827768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAY6139904.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6521..6739
                     /locus_tag="JQH65_002524"
     CDS             6521..6739
                     /locus_tag="JQH65_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HAY6139905.1"
                     /translation="MQDLSLEARLAELESRLAFQESTIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6788..7378)
                     /gene="slyD"
                     /locus_tag="JQH65_002525"
     CDS             complement(6788..7378)
                     /gene="slyD"
                     /locus_tag="JQH65_002525"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAY6139906.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7473..7673)
                     /locus_tag="JQH65_002526"
     CDS             complement(7473..7673)
                     /locus_tag="JQH65_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HAY6139907.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7683..9494)
                     /gene="kefB"
                     /locus_tag="JQH65_002527"
     CDS             complement(7683..9494)
                     /gene="kefB"
                     /locus_tag="JQH65_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HAY6139908.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEE
                     DEKPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYY
                     GDATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHE
                     LLQAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHA
                     DTVQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9494..10048)
                     /gene="kefG"
                     /locus_tag="JQH65_002528"
     CDS             complement(9494..10048)
                     /gene="kefG"
                     /locus_tag="JQH65_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HAY6139909.1"
                     /translation="MISQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAH
                     YPDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLA
                     GKYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQS
                     AKELANHARAYGDWLANPLSPGGR"
     gene            10176..12089
                     /locus_tag="JQH65_002529"
     CDS             10176..12089
                     /locus_tag="JQH65_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY6139910.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALSQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12089..13111
                     /locus_tag="JQH65_002530"
     CDS             12089..13111
                     /locus_tag="JQH65_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAY6139911.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVIFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13105..13323
                     /locus_tag="JQH65_002531"
     CDS             13105..13323
                     /locus_tag="JQH65_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HAY6139912.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13377..14246
                     /locus_tag="JQH65_002532"
     CDS             13377..14246
                     /locus_tag="JQH65_002532"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HAY6139913.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14301..>14560)
                     /locus_tag="JQH65_002533"
     CDS             complement(14301..>14560)
                     /locus_tag="JQH65_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HAY6139914.1"
                     /translation="IDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTG
                     RDLKDAAVARAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
BASE COUNT         3599 a         3968 c         3587 g         3406 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgattgtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt taatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccgga ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccatgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt cacctatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcgaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagaccca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcacttgcc
     4441 ggtgaggtca attggttagc gttatagacc cctccccgat agaaaaagac gctgcttaac
     4501 tcatggcctt ctactatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctggaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaaa cactcctttt
     5341 caggagcctg tcgttaactt ttcagggcag gctcattaat gatgcgggta actaaattaa
     5401 tacagcggag gttccgcttt ccagcactaa ttatatccgg cctgtaataa aaaaaccgcc
     5461 gcctggtcag gcggcggttc ttaatgctta ttttttagca gaatctgcgg ctttcgcatc
     5521 agcttccggc tttgcatcag ccttcggtgc tggtttcaca tccagcagct ctacgtcaaa
     5581 caccagggta gaattcggtg ggatccccgg aacacccgct ttgccgtaag ccagttctgg
     5641 tggaataacc agtttgatct taccgccttt cttgatgttc ttcagacctt ctgtccaacc
     5701 cgggataaca ccgtccagac ggaaagaaag cggttcacca cgggtgtaag agttgtcgaa
     5761 ctctttaccg tcgatcagcg tacctttgta gttcactaca acagtatcgc tgtctttcgg
     5821 tgcttcgcct ttaccggctt ctactacctg ataaaccaga ccagttgaag aggttttcac
     5881 acctttctct ttggcaaatt tctcgcggta ctctttacct tttgcttcgt tatcagccgc
     5941 gtctttttcc atcttcgcct gagcagaaga cttcacgcga gcttcgaatg cttgcagagt
     6001 ctgttcgatc tcttggtcgg agagtttgct cttatcagca aatgcatcct gaacaccagc
     6061 gatcagctga tctttatcca gtttgatgcc cagtttttct tgttctttaa gagagttttc
     6121 catgtaacga cccagcgaag cacccagtgc ataagctgat ttctgatcgt catttttgaa
     6181 cgctgctttg ctgtcagcag ttgtagcagg ttttgcagct tcagcagcaa aagtgattgg
     6241 tgcatgcagg gcaacggcca ttgtggtcgc cagcagcgtt actttaaaca gtgatttcat
     6301 ccatatctcc aggatcgggg catctcaccc cagggttaac tattatcaga agggtactat
     6361 aaagcgttgt cgaacaaatc tacatacaga cacgccctat tatcatctat tttcagactc
     6421 tttttgttta aattagtttc gatgaccgcg aaatgagtgc tgtctcgggc agcaaagtta
     6481 agtagaatcc gcggcaacca ttcgacaaaa gaggtgaatc atgcaggatt tatcattgga
     6541 agcacgcctg gcagagctgg agagccgact ggcttttcag gaaagcacca ttgaagaact
     6601 gaacgtcacg gtgaccgctc atgaaatgga gatggcgaaa ctgcgcgatc atctgcgtct
     6661 gctgaccgag aagttaaaag ccagccagcc gtcgaacatc gcgtcgcagg ctgaagaaac
     6721 gccaccgcca cattattgag gcgtaaaaaa agcggggatt ccccgctttt ttgtcacctt
     6781 ttcggtatta gtggcaacca caaccgccgt tgcctttacc gccacagcag ccttcgccac
     6841 cgtgttcatg accgtgatcg tggccatgac cgccgcagca accgtcgtgg tcgtgatcgt
     6901 ggtgatgatc gtgcgcgccg tgaacgtgac catgagccag ttcttcttcg gtcgcttcac
     6961 gaatcgccac aacctcaacg ttgaatttca ggttctgacc ggccagcatg tggttaccat
     7021 caaccactac gtgatcgtct tcaaccgcag tgatttcaac cggtaccggc ccctggtcgg
     7081 tttcagccag gaaacgcata cctacctgca gttcatcaac gcccataaat acgtctttag
     7141 gaacacgttg caccaggttt tcgtcgtact gaccgtaagc gtcgttcgcg ccaacagcga
     7201 catcaaattt gtcgccaact tcatgacctt ccagcgccgt ttccaggcca gagatcaggg
     7261 aaccgtgacc atgcaggtag tccagcggcg cactcaccgg agactcatca accaacacac
     7321 cgtcttctgt acgtacctga taggccaggc tgaccaccag gtcttttgct actttcatga
     7381 tatctcctga gcatgggaag aatagtggcg cagattgtag cggaattctg cagccgtgta
     7441 ctcactagct taaaaaaact cggcgcatat cgctagtccg gatgaaaaat cccgatcact
     7501 tgctcatctt tgcgaacgtg atcgcgggct tctttgtctg cttctcgcat ctgatgtccg
     7561 cacttaacgc attcaacaat atcaatatta ttttcgcgcc acatcgccat tgaatcctgt
     7621 gcctgacagg ccgggcattt tgcgcccgca ataaaacgtt ttcggattgc catctttacc
     7681 ctctactcaa attcatccca gccgtccagc tggcgtcgtt cttgttgcat ttcacgctgg
     7741 aaaatctctt ccagttcgcg tcgggcttcc ctggcgcgag aaatttgtac agtatcggca
     7801 tgcattggga tgagctctcg cagcatccgc atatccaggc ggcgaaaatg cagttgcgcc
     7861 cgctgcgcct gatgcggatg catcccaagc gtgaccaaag tcttgcgccc cagctctaac
     7921 gcactggaga atgtttcacg ggaaaactgc gtcacccctg cctgtaataa ctcatgcgct
     7981 tccacacgtc cgcgcgctcg cgcaagaata tgcaaatgcg gaaagtgctg ctggcatatt
     8041 tccaccagct tcatggtgtc ttccggctcg ttacaggtaa tgacgataga ctcagcggcc
     8101 tctgcacccg cagaacgtaa tagatcgacc tgcgtggcgt cgccgtaata aactttgtag
     8161 ccatatttac gcatcaggtt aacggcgctg atatcccgct ccagcacggt aatgcgcatt
     8221 ttatttgcca tcagcaaacg accaatcacc tgaccaaaac gcccgaagcc cacgacaatg
     8281 acctggggtt tatcatcgtt gacccacggt ttttcgtctt cttcttccgg tccgttaaac
     8341 tggcgggata accatttatc caccagcttc atcagcaacg gcgtggtcat catggaaagc
     8401 gtcaccgtca ccgtcaccag caacagcgcc atctggtcgc cctggaataa acgttgtgaa
     8461 gaagcggtag aaaagaggac aaaggcaaac tcaccgccct gactcaacac gccagcaaac
     8521 tgcatccgct ctgagctacg cacgccatac aatcgcgcca gcagatacag cacgagaatt
     8581 ttcaccgcca ccagcacaac cacgcttatc actacccaca acagatgggt ataaagcacc
     8641 ccgaggttga gcgacatgcc gacagagata aagaacaaac cgagcagcaa gcctttgaag
     8701 ggatcgatag ccgtttccag ttcatggcga tattcacttt ccgccagcag cacgcccgca
     8761 ataaatgtac cgagcgccat cgacagcccc agcgcatcca taaacaatgc ggaacccaac
     8821 accagcagca gcgtcgcggc ggtgaacact tcccgcacgc cagaagctgc aataaagcgg
     8881 aataccggac gcagtaaata gcgcccacca atcagcatgc cgacaaacgc cagcaccttc
     8941 atgccgatct tcatccagtc gaaatgttcg tctgccgacc ccgccaacaa cggcactaac
     9001 gccagtgctg ggatcactgc cagatcctga aacagcagaa ccgaaaaccc gagctggccg
     9061 gattcgctgc gattcatccc tttctcacgc atcaattgca acgccattgc agttgaagac
     9121 atcgcaaggc caatgccacc gaccaccgcc gcctgccagg cgaaatccgt cagcatcaat
     9181 aatcccgcca gcaacgccgc gcttaacagc acctgtgccg cgcctacgcc aaaaatcgaa
     9241 cgccgcagtt gccaaagttt ggagggattc aactcaaggc cgatgataaa catcaggaat
     9301 accacgccga gttccgaaaa gtggaggatc tcatcgacgt cgctaataaa ccccagcccc
     9361 cacgggccaa ttgcaatccc tgccagcaaa tatcccaaca cagcgccaat ccccagccgc
     9421 gatgccaacg gcaccgcagc caccgccgcg aagagaaaca gcactcctgc gagtaaaaaa
     9481 tcggaacctt ccatcagcgg cctcctggag acagcggatt tgccagccag tcaccgtagg
     9541 ctctggcgtg gttcgccagc tcctttgcgc tttgccgtct cgcccagtaa ataatgatgg
     9601 gacttaacca atgcatccgg cacatgcccg ccgccagttc aaaggggcgc agcacatcgc
     9661 tcatcgggta gcgattcagc gcgtcataac ggtaagcact ttccggctcg ccggtggtaa
     9721 tcacgttacg ccagtacttt cccgccagtt ggtttcctcc cggcccgctg gcaaaaccac
     9781 gacttaatac ccggtccagc cactctttca gtagcgccgg gcagctatag gtataaagag
     9841 gatgctgaaa gacaatcacc tcgtgctcgc gcagtaatgc ctgctcacgg gggatatcaa
     9901 taaaaaaatc gggatagtgc gcgtaaaggt cgtgcacggt aacattgctg agctgcgtgg
     9961 ccggtttaag cagtacccgg tttgccaccg agtcctgaga ttccggatgg gcatacagca
    10021 gcaaaacttt cgctggctga gatatcatcc ccctcccggt atggtttttg tgtatagtcg
    10081 ctgttttggg ctaccattgc gcccggtgcg gcagctcgcc catacattac attatcataa
    10141 tgataagtta acatagtctg aacatacggc gccttatgat tgttttctcc tcgttacaaa
    10201 ttcgtcgcgg cgtgcgcgtc ctgctggata atgccaccgc caccatcaac cctgggcaga
    10261 aagtcggcct ggtgggtaaa aacggctgtg gtaaatctac cctgctggca ttgctgaaaa
    10321 atgaaatcag cgccgacggc ggcagctaca cctttccggg aagctggcaa ctggcgtggg
    10381 tgaatcagga aacgccggcg ttatcgcaag cggcgctgga atatgtcatt gacggcgacc
    10441 gtgaatatcg tcaactggaa gcgcagctgc acgacgccaa cgaacgtaac gacgggcacg
    10501 ccattgcgac cattcatggc aagctggatg ctattgacgc atggagtatt cgctcccgtg
    10561 ccgccagcct gctgcacggc ctcggtttca gcaatgaaca actggagcgc ccggtaagtg
    10621 atttctccgg tggctggcgt atgcgtctta accttgccca ggcgctgatt tgccgttcag
    10681 acttgctgct gctcgacgaa ccgactaacc acctcgatct cgatgccgtt atctggctgg
    10741 aaaaatggtt gaagagctat caaggcacgc tgatcctgat ctctcacgac cgcgacttcc
    10801 tcgatccgat cgttgataaa attattcata tcgaacaaca aagcatgttc gagtacaccg
    10861 gcaactacag ttcgtttgaa gtacagcgcg ccacccgtct ggcgcaacaa caagcgatgt
    10921 atgaaagcca gcaggaacgc gtggcacatc tgcaaagtta tatcgaccgt ttccgtgcca
    10981 aagccaccaa agcgaagcaa gcccagagcc gcattaagat gcttgagcgt atggagctga
    11041 ttgcccccgc gcacgtcgac aacccgttcc gctttagctt ccgcgcgccg gaaagcctgc
    11101 caaatccgtt actgaagatg gaaaaagtca gcgcgggcta tggcgatcgc attattctcg
    11161 actcgattaa actgaacctg gtgcccggct cgcgcattgg tctgttaggc cgcaacggcg
    11221 cgggtaaatc gacattaatc aaactgttag ccggtgaact tgcgccagtc agcggtgaaa
    11281 ttggtctggc gaaagggatc aagctcggct acttcgccca gcatcaactt gaatacctgc
    11341 gcgccgacga atcacctatt caacatctgg cacgtttagc gccgcaggag ctggaacaaa
    11401 aactgcgtga ctacctcggc ggctttggtt tccagggcga taaagtaacc gaagaaacgc
    11461 gccgcttctc cggtggggaa aaagcccgcc tggtgctggc attaattgtc tggcagcgtc
    11521 cgaatctgct gctgctcgac gaaccgacta accaccttga cctcgacatg cgtcaggcac
    11581 tcaccgaagc attaatcgag tttgaaggcg cgctggttgt cgtttcgcac gaccgtcatt
    11641 tgctgcgttc caccactgac gatctctacc tggttcacga tcgtaaagtc gaaccgttcg
    11701 acggcgatct ggaagattat caacagtggt tgagcgacgt acaaaagcag gaaaaccaga
    11761 ccgacgaagc gccaaaagag aacgcgaaca gcgcccaggc acgtaaagat cagaagcgtc
    11821 gggaagcgga gctgcgtgcg caaacccagc cgctgcgtaa agagattgcc cgtctggaaa
    11881 aagagatgga gaagctgaac gcgcaactgg cgcaggcgga agagaaactc ggcgacagcg
    11941 aactgtatga ccagagccgt aaagcggagt tgaccgcctg cctgcaacag caagccagcg
    12001 ccaaatccgg cctggaagag tgcgaaatgg cgtggctgga agcccaggag cagcttgagc
    12061 agatgctgct ggaaggccaa agcaactgat ggcgcagata acgacgaccg atgccaatga
    12121 attcagcagc agtgctgaat tcacccctat gcgcggcttt agcaattgtc atctacaaac
    12181 tatgctgccg cgtttgtttc gtcgtcaggt gaaattcacc ccgtactggc agcggctgga
    12241 gttgcccgac ggcgattttg tcgatctcgc gtggagtgaa gcccctgcac aggctcggca
    12301 taaaccgcgt ttagtgatat ttcacgggct ggaaggcagt ctcaatagcc cttacgccca
    12361 cggtctggtt gaggcggcgc aaaagcgcgg ctggctgggc gtggtgatgc attttcgcgg
    12421 atgcagcggt gaaccaaacc gtatgcaccg catttaccat tcgggcgaaa ccgaagacgc
    12481 cagttggttt ttacgctggc tgcaacgcga atttggtcat gcgccaacgg ctgccgtcgg
    12541 ctattcgctc ggcggtaata tgctggcctg tttgctggca aaggaaggca atgatctccc
    12601 ggttgatgcg gcggtgattg tctctgcgcc gtttatgctg gaagcctgta gctatcatat
    12661 ggaaaagggc ttttcccgcg tttatcagcg ttacttgctg aacctgttaa aagccaatgc
    12721 cgcgcgcaag ctggcagcct accccggaac gctgccgatt aatctcgcgc agttaaaatc
    12781 ggtacgtcgc atccgtgaat ttgacgatct aatcaccgcc agaattcacg gctacgccga
    12841 cgctatcgac tattatcgtc agtgtagcgc catgccgatg ctgaaccgga tcgccaaacc
    12901 gacgctgatt attcacgcca aagacgatcc gtttatggat catcaggtga tcccgaaacc
    12961 ggaaagtctc ccctcgcagg tggagtatca actgactgaa catggcggtc atgttggctt
    13021 tattggcggt acattacttc atccgcaaat gtggctggag tcacgcattc ctgactggtt
    13081 aacaacgtat ctggaggcga aatcatgttg attccgtggc aagacctctc ccccgaaacg
    13141 ctggaaaatt taattgaaag ctttgtgtta cgtgaaggca ccgattatgg tgaacatgag
    13201 cgtacacttg aacagaaagt cgccgacgtc aaacgccagc tacagtgcgg agaagcggtg
    13261 ctggtatggt cggagctgca cgaaacggtc aatattatgc cgcgcagtca gtttcgcgaa
    13321 taacctgcaa ccgtcggcgg acgagataat aatgatagct aagtcatgga gttactatgt
    13381 ctgccaaaca tccggtcatt gcggtaacag gatccagcgg cgcggggacc accaccacca
    13441 gcctcgcgtt tcgtaaaata ttcgcgcagt taaatctgca tgcagctgag gtggaaggcg
    13501 acagttttca ccgttacacc cgcccggaaa tggacatggc gatccgcaaa gcgcgcgacg
    13561 ccgggcggca tatcagctac ttcggccccg aagcgaacga cttcggcctg ctggaacaaa
    13621 ccttcattga atacggtcag agcggcaaag ggaaatctcg caaatatctg catacctacg
    13681 acgaagccgt accgtggaat caggtaccgg gaacattcac cccctggcaa cctttaccag
    13741 aacccactga tgtactgttt tatgaaggtt tacacggcgg cgtagtcacg ccacagcata
    13801 acgttgcgca gcatgtggac ttactggtcg gcgtagtgcc tatcgttaac cttgagtgga
    13861 ttcaaaaact gatccgcgac accagcgagc gcgggcactc acgagaagca gtgatggact
    13921 cagtagtgcg ttcaatggaa gactatatca actacatcac accgcagttt tcccgcaccc
    13981 atcttaactt ccagcgcgtt cccaccgtcg acacttcaaa cccgttcgcg gcaaaaggta
    14041 tcccgtcgct ggatgaaagc tttgtggtga tccattttcg taatctggaa gggatcgatt
    14101 tcccctggct gctggcgatg ttgcaaggct cattcatttc ccacatcaat acgttagtgg
    14161 taccgggcgg caaaatgggt ctggcaatgg aattaattat gctgccgctg gtgcaacgat
    14221 tgatggaagg aaagaaaatc gagtaactct gctattacgc cggataaaat actatccggc
    14281 ttcacaacgg gatagttaaa tcacgcggca accacttcat acgagtgagt aatattcacc
    14341 gctttttcca gcatcaacgc cactgaacaa tatttctcgg cagagagatc aaccgcacgc
    14401 gcaaccgctg cgtctttcag gtcgcgaccg gtgacgataa aatgcagatt aatgtgcgta
    14461 aacaggcgtg gtgcctcttc gcggcgttca gaggtcaatt ttacttcaca atcgaccaca
    14521 tcctgacgcc ctttttgcag gatcgaaacc acatcgatgg
//
LOCUS       DADLFA010000099        14429 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000099 DADLFA010000000
VERSION     DADLFA010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14429
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.099"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..787)
                     /gene="fimH"
                     /locus_tag="JQH65_002534"
     CDS             complement(<1..787)
                     /gene="fimH"
                     /locus_tag="JQH65_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HAY6139915.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLG"
     gene            complement(807..1310)
                     /gene="fimG"
                     /locus_tag="JQH65_002535"
     CDS             complement(807..1310)
                     /gene="fimG"
                     /locus_tag="JQH65_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HAY6139916.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGETKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(1323..1853)
                     /gene="fimF"
                     /locus_tag="JQH65_002536"
     CDS             complement(1323..1853)
                     /gene="fimF"
                     /locus_tag="JQH65_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HAY6139917.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(1863..4499)
                     /gene="fimD"
                     /locus_tag="JQH65_002537"
                     /pseudo
     CDS             complement(1863..4499)
                     /gene="fimD"
                     /locus_tag="JQH65_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein FimD"
     gene            complement(4566..5291)
                     /gene="fimC"
                     /locus_tag="JQH65_002538"
     CDS             complement(4566..5291)
                     /gene="fimC"
                     /locus_tag="JQH65_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HAY6139918.1"
                     /translation="MSNKNANVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWMENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQFAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(5328..5867)
                     /locus_tag="JQH65_002539"
     CDS             complement(5328..5867)
                     /locus_tag="JQH65_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY6139919.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPVNWKRLYSGSTSLHFIAKYRATG
                     RWVTGGIANAQAWFSLTYQ"
     gene            complement(5932..6480)
                     /gene="fimA"
                     /locus_tag="JQH65_002540"
     CDS             complement(5932..6480)
                     /gene="fimA"
                     /locus_tag="JQH65_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/fibrin-binding adhesin FimA"
                     /protein_id="HAY6139920.1"
                     /translation="MKIKTLAIVVLSALSLSSTAALAAATTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQIVQLGQVRTASLAQEGATSSAVGFNIQLNDCDTNVASKAAVAFLGTAID
                     AGHTNVLALQSSAAGSATNVGVQILDRTGAALTLDGATFSEQTTLNNGTNTIPFQARY
                     YATGVATPGAANADATFKVQYQ"
     gene            complement(6957..7553)
                     /gene="fimE"
                     /locus_tag="JQH65_002541"
     CDS             complement(6957..7553)
                     /gene="fimE"
                     /locus_tag="JQH65_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria regulatory protein FimE"
                     /protein_id="HAY6139921.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTLERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(8031..8633)
                     /gene="fimB"
                     /locus_tag="JQH65_002542"
     CDS             complement(8031..8633)
                     /gene="fimB"
                     /locus_tag="JQH65_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria regulatory protein FimB"
                     /protein_id="HAY6139922.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTPPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
     gene            10089..10805
                     /gene="nanC"
                     /locus_tag="JQH65_002543"
     CDS             10089..10805
                     /gene="nanC"
                     /locus_tag="JQH65_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HAY6139923.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSSDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            10825..11931
                     /locus_tag="JQH65_002544"
     CDS             10825..11931
                     /locus_tag="JQH65_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HAY6139924.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNREGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAVDKINAYYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQHGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            11996..12976
                     /locus_tag="JQH65_002545"
     CDS             11996..12976
                     /locus_tag="JQH65_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HAY6139925.1"
                     /translation="MDAIISPDYYYVLTIAGQSNAMAYGEGLPLPAREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLSFIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLIMGYYGSAYRSPENWTTALRSSHFSAAARRGIISDRFVEAILQFWRGK"
     gene            complement(12984..13106)
                     /locus_tag="JQH65_002546"
     CDS             complement(12984..13106)
                     /locus_tag="JQH65_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012139841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY6139926.1"
                     /translation="MISFVFLLLVCPNCHAMLHRRKPPFTPEELKALMDANKSN"
     gene            13188..14060
                     /locus_tag="JQH65_002547"
     CDS             13188..14060
                     /locus_tag="JQH65_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY6139927.1"
                     /translation="MASSKSLQQAIANIKIWHKGEQRAPHKPLLLLYVLAGYLNGHPR
                     LFDYGSEIYEPLHSLLERFGPQRSQYRPDMPFWRLQGDGFWQLHNAELCSTAGSSRQP
                     PVKELNEYHVAGGFDEQHYALVTGNKKLINTLAQQILEAHFTESIQEEIADELGFDLQ
                     QIRKQRDPLFRKNVLRAYNYQCAICGFNMRHDDTTVALEAAHIKWKQHGGPCEIPNGL
                     ALCAIHHKAFDKGSIGLDENMRVLVSDAVNGGGSVERLFWDFDGKIIALPQVRKNYPY
                     EGFVEWHRNEVFRG"
     gene            14105..14284
                     /locus_tag="JQH65_002548"
     CDS             14105..14284
                     /locus_tag="JQH65_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6139928.1"
                     /translation="MQDLAASLQLAFDSGAKRVLLPISWAMDIPTVPAELFTKFQVSF
                     YSEPVDAVYKALDVN"
BASE COUNT         4043 a         3328 c         3151 g         3907 t
ORIGIN      
        1 ctcctaacga taccgtgtta ttcgctggaa taatcgtacc gttgcgcgtc aactgtacgc
       61 cgacgccctg cgctggtgaa aacgacgcgg tattggtgaa aatcgagttg cccgcatctg
      121 cggttgtgcc ggagaggtaa taccccaggt tttggctttt cgcacaataa acggtaagag
      181 gaattggcac tgaaccaggg tagtccggca gagtaacggt gacatcacga gcagaaacat
      241 cgcagccgcc agtaggcacc accacatcat tattggcgta aatattccac acaaactgga
      301 aatcatcgct gttatagttg ttggtctgtc gcaaaataag cacggcaatt aatgagccag
      361 ctttaatcgc caccccgccc gcactgctca caggcgtcaa ataaagcgcc accggccacg
      421 gcttatccgt tctcgaatta taaacaaccc gcggcgtttc gctggtggtc ggaaatggat
      481 agctactgcc actatatttt acggtcccgg aaaaattaga taacacgccg ccataagccg
      541 agcctcgttg cagtgtgaca tagtctgtaa tggtttccgg ataatcgtta tggcaaaaga
      601 tttgcgtcga aagatctacg accaggtttt gccccacatt cacgacaggc gcaaggttta
      661 cataaacatt agcgctgcca ccgccaatag ggatagcagt accattggcg gttttacagg
      721 cgaatgacca ggcatttacc gaccagccca tcagcagtac agcaaacagg gtaataactc
      781 gtttcattac aattatctct tcgggttcag ctgtaggtat aggtgatgct aatcactgcc
      841 tgaatggttc cctgagtggc tccgccattt accgtcaatg ctctgacctg taacgggaag
      901 tgcgctgatt gtgaggaatc atccacctga actgttttgg tttcgccagt attcaatgtg
      961 ttgccactgt catcctgtag ctctaactgg atgttttgcg cggtcccctg gtttttataa
     1021 tatccggtac tgtcggctgc cccgctgaag ctggcagtga ccctcgacgt tcccaccgga
     1081 caattagtca actcaagcgc aacatcatgc caggccgatg ccgccccggc agacatcaga
     1141 ctgaaagaat aaagatcgcc gagatcaacc gtggcattgg tggtggaaac tgtgcacggt
     1201 ttggcgacga ccttaccgtt caccgtgatg gtgacatcgg ctgcctgtat tgtcgcactt
     1261 gcgagcgcca atattgccgc caataaatac ccacgtttgc gccatttcat gagcatctcc
     1321 agttactgat attcaagagt gaaggtagcc gtggcattga tatgccccgc agtgacaggc
     1381 acctgtgtcg ccattagccg ggcgtaaaaa ttcaacgtat ttggtttacc cggcgtcagg
     1441 gtcgtccacg aaatcgcgga cgatggagca ttaaggggta tctgattttg ctgctcattc
     1501 agaagctgta ttcccagtcc cgaagccgct gacaccgtat tttcaagtgc aagcaggttg
     1561 gcattgtggc tatctgcaac gccggtaaac ccaaccttta cggcagaaac ggcgttacca
     1621 cagggtgaca gcaaaatacg aaatggaacg acaggagtcg ttgcgccaat gttgttaaat
     1681 tgcttcgccg cgttttccat cagatcaaca gtaaaattgg ttgattcagc ggccacacta
     1741 cagccgttat ctctgacata gccgcggata gtaatcgtgc tatccgcagc caaaacgcga
     1801 cttaccgcca gccacaaaaa agcgcacaga agataaaaag gtttgtttct catcacgccc
     1861 ccttaacgac attcagctga tagctgggtt aataactgct gctgactctc tggtggcagt
     1921 tgataattgg cgatacagtg agcattttcc tcttctcccc atttcacctg aacttttccc
     1981 gctaaaggca ttccgctgag gtaaacctga ccattatccg caacaatgcc gctactctgg
     2041 ctactctctg atgtcaccat cgccccaaac ggcagcggct tattattgtg ggttagcgtc
     2101 atgagcagtt ttatcccaac gcgcgcttta aactctgctc gcacgatcgc cccacgagtg
     2161 ggaacaacgt tagcgaccgc gttatctaaa tcgacgttat cagccagggt attggtatcc
     2221 agcgccactc tgttttcccg atattcggtg gcataaggca gcacggcata accgcgccag
     2281 tcggtacgca cccccgtctg gttttcgact tttgcatctt ttgcgccagg cgctttaaca
     2341 agtaccaccg tatcgtttaa cggctgcccc agcgttacgc cattggcatg agccagtacc
     2401 ccaccgctga ctccgtaata gagctgctta atatcatcgc tatggctgta accgatattg
     2461 gcattgccgt aaccaccgcg ataattcagc gtggcgtagc ctgtacttcc gctattacca
     2521 tcgcctcccc cggcatagcc ggtttgcacg ctatagctga ggttgttgtc ttccagcaac
     2581 gtaccgtata caccagccag attggtcatc cgaccgttga gatcgtgtga cacgctgtag
     2641 ctggcactgg catgtcgcca ctgagattta ctgtcagaac gcagccagtg gctgaaagga
     2701 atattgacgt taagcgctaa catctgatcc cgcccttttt gccaggcgtt tttcgtcagg
     2761 ctatagctga gcgtccagtt gatatcttcg aacgcagtat ttaatccagc ctagaattgc
     2821 tcatcgacat tactcgttcc ccaataagtt tgatggctac cactcaaata cagtgttgat
     2881 gtgcgcccga gttgctgagt aacggtgagc tgtaatttcc cgcgtttgtt ataagcgagg
     2941 ttgtaatagt cggtgaattt cggcttaacc tgaataactc catcctgtgt ttcgatgttg
     3001 tagccattca ttcgactgta tgttgtatca gcgaaattaa aatatccgct ggtcgaataa
     3061 cggtaaccca ctaactgaat attcgtgcct gactcattga gcgatttgtt atagagaaaa
     3121 cgcaccgatt gtccgtcatg ctgactgtca tcgggaagtg tggaattagc ctgcgtcata
     3181 tccacagaca gagcgcccag tgcccccatg tttttcccga taccgaaatt aaaagcacga
     3241 taacgatccg ccagttgcgt tccaccatat attgtccagc cagccggaag gccgtggagt
     3301 aatgtacttt ggaaaaagcg gggtttttcc tgctgcgtat ttccactacg gtattctcct
     3361 gccgtaatgg aataacgagt atgcccttca cgttgcaaaa gcgggactga cgaatagggt
     3421 acggtaaaaa tctgcgtgct gccgtcagcc tctttgatcg ttacctgcaa gtcaccacta
     3481 ttacctgcgg catagatatc gttgatggta aaaggccccg gtggcaccgt actattataa
     3541 atgtcatacc cattttgttt aatagtgacc tgtgcagtac cacgagcaat accgtggatc
     3601 accggggcaa atcctctttg gctatcgggt aacatattgt catctgaggc caattgtgcg
     3661 ccgcgaaagt taataccatc gaaaatatcg ccctgggtat aaccatcacc cagcgtcagc
     3721 cgggaacgta acggtattat gtctcgctca agccaggtat tgatatgctg ccatttattt
     3781 ttgctacctg atgatctgtc gctactgtta taactccagc tggtattgtc gcgtaaacac
     3841 cacgcaccaa tatttaaccc actctgtagg tttaaatatg cataatggct gttaccccca
     3901 atctgattct gtacactatt tccgctgaaa ttataattga gcaatccggc attaataccg
     3961 ggatcccata actcaggagg aatataacca cgcgcgcgat tactcataaa tgcctgaggg
     4021 atcgtcaggt tcagtcgctg ctgaccaaca tccagatgcg cagtagcgtc atggatcatt
     4081 gaggttaatg gcacgcaggc atcatctgcc agcagattca taccggagac agaagccgca
     4141 ttcagcccca tactggcaag ttgcgcgcgt gtcaggcagg gaacaatccc ttgttcactg
     4201 tcgcccgtat taaatgtgac atcacgcgtt gccatataac cattattcaa atagatatcg
     4261 acgcgatacg tccctggcgg taattcttgc ccattttcaa aacgcgataa atcggccaca
     4321 gcctggggat catccgctaa aaagcgcgga ttaaaataga gttcggcaga tgacaaaggt
     4381 gcctgtgcgg caaaagcaca ggcgacaacg agccggacaa aaaaaccagc caaacgatgc
     4441 ttacgaatat gcaagcattg tgtgtttcgc tggtaaagtc ttaaattcag atatgacatt
     4501 attcctccgg tttaaaatta cccggcagtc aattcttttt attcaggcga gaaattcccc
     4561 ctgcgttatt ccattacgcc cgtcattttg ggggtaagtg cgccataatc atttattgtt
     4621 cggtaagtaa tattgcttcc tgcatcagaa ggcaatttaa ccgtgctttc gcccattgga
     4681 ggcaccaatg cattttcaag aacccgggtt ccggcattca actctgttac cgtcagataa
     4741 tagggtgtcg ggttaatcag cgtcagagaa ttcgcgctac gacgaaatct taatttttct
     4801 gcggcctgat cgggtggcaa cgctaattta gccgggcgat agtacagttt aatgcggctg
     4861 ataattgcga actgtagcgt attctcagtc aattttgatt tatccattga cggaatcgct
     4921 ttaacattca tccagaataa actttcccgg tcctgtggca attggttatt tgttgcatca
     4981 agaatacgca aggtattctc tttttttccc ttcatcgcaa acagaggagg cgtcacgata
     5041 aaacgaccat cctttacacc atcggcattt tccatccatg attgaattaa ataggtacta
     5101 ttttcatcat tatttgtcac ggcaagttgc acttgttttt gccctgccgg ataaattacg
     5161 cgagtcgcac ctaaggccac tcccgcttca gcgcgtccgg caaccatcat tgccatgaac
     5221 atcaggatac ctgccagcaa gcagaatgtt atttcctgcg atttccttac attggcgttt
     5281 ttattactca ctgtcctgtt cctgttatca cattatctgc tgaacaatta ctgataggtt
     5341 aaagagaacc aggcctgggc attggcgatg ccgccagtaa cccaacgccc ggtagcacga
     5401 tatttggcga tgaaatgtag cgaagtagag cctgagtaaa gccgtttcca gtttactgga
     5461 ggacgattaa tcggtacgag gtttccttca tcatcaaaca acgctacgcc aatattggtg
     5521 gctatccctg gcccctctcc cacggaaagc acatccggat ttttaccatc cgcgacaccg
     5581 tgaaacgcca cacccacacg ttcactcacc accgtgctac attcccgtaa atgaataaca
     5641 aaaggcaccg gtgcgctatc ttcccctacc gcatgaaacc ggttactgct gatttgcccc
     5701 atattgaccg tcatttgttt atcaccggct tcaatccggc aagtttccgc aataatgacg
     5761 ccctgaaatt gcatatttcc gccgggcagc gtagtattcc atttatttcc ggccagagca
     5821 aacattggca gcaacgacgc taatagaaat aatctcttcc ttttcatcgt tatttttatc
     5881 gcacaagggt gggcatccct gcccgtaatg acgtccctga acctgggtag gttattgata
     5941 ctgaaccttg aaggtcgcat ccgcattagc tgcgccaggg gttgcgacac cggttgcata
     6001 ataacgcgcc tggaacggaa tggtgttagt accgttattc agggttgttt gctcactgaa
     6061 tgtcgcacca tccagcgtca gcgcagcacc cgttctgtcc aggatctgta caccaacgtt
     6121 tgttgcgcta cccgcagctg aactctgcag agccagaacg ttggtatgac ccgcatcaat
     6181 cgccgtacct aaaaaggcaa ctgcggcttt agatgcaaca ttggtatcgc aatcattcag
     6241 ctgaatgtta aaaccgacag cagaactggt tgctccttcc tgtgccagcg atgcggtacg
     6301 aacctgtcct aactgaacga tttgatcaac agagcctgca tcaactgcgc aagcggcgtt
     6361 aacaacttcc cctttaaagt gaacggtccc accattaacc gtcgtggcag cggccagagc
     6421 cgctgtagaa ctgagggaca gagccgacag aacaacgatt gccagagttt taattttcat
     6481 gctgctttcc tttaaaaaaa ctatttctaa atcgacatgg gcagtcgttc tgtacacttt
     6541 gttttgtcaa cgagtttgac tgccaacact gcacagtttt cccccaaaag atgaaacatt
     6601 tggggccatt ttgactcata gaggaaagca tcgcgcacaa actttttcag tttatttgtt
     6661 ggcttaatat tctattgtta tctttattta tagattttta tattgcatga ggtggttttt
     6721 ggagagaaga atgaggaaga tgcgtcgagc cacagaaacg ttagctttac atatagcgga
     6781 ggtgatgtga aattaattta caagagaaat aatttacata tcaaacagtt agatgatttt
     6841 tgtcgttttt taaaattttt atgcttgaga aaaaatacgt aacttattta tgatatggac
     6901 aatttggccc caattgtctt gtatttattt tttaacttta ttatcaataa gttaattcaa
     6961 acctcttctc tttttaattt ttcgtttatg agattatttc tttcccataa tccggcaaaa
     7021 cgagcagcat tactggcggt ataacgtaca gtatggcgaa tatttcgatg cccgagataa
     7081 tcctgaatta aacgagtatc tgcaccacgc tccgccagtt cataaccgca agcatgcctt
     7141 aacatatgag gatgagtctg cgttacggtt ccagcttcaa taccggcatc acgaataatg
     7201 cgataggcct gctggcgaga aagccgactc ccgcggcgag aaataaatat ggcgtcggtc
     7261 cggtcagcgc ctttccagtt agcacgttcc agggtccagc gttccacagc ttcacgctca
     7321 tcaaaacgta acgggtgaac ggtagaaaat ccgttcttca gtcggcgaat atttattcga
     7381 ccttcattaa ggtcaagatc ctgataatgc agatcaagca gttcactaat acgcatccca
     7441 tgccgatatg ccaacagaat aagacaataa tctctggctc ccgttgcccc gtaacaaacc
     7501 gcctgcatca tggcctgaac ttctttaccg gtaagataac gacgtttact cacaatagta
     7561 gtactcctaa ctgagatata tttgaatgaa tacctatagg aaacctcaat cggtcaaaat
     7621 tagcccaata gataaaaaga taaccacaca cgaagtgatg tggctatcag tcaattacat
     7681 taaatatcat acaaaaataa aatatcactg atgtgacaac atctattttc attgatttga
     7741 ttcaatataa caatagattg tagtagatta gcttccagtt gcttaaaaaa taaagaaaaa
     7801 agtgcgaaaa caggagaatt taagtgatgt gtaaatttat gaatgatttt gtttatttat
     7861 tttgaagaac ataaaccatt gtattttgta tggatatggt taacttaatg cttattatga
     7921 taattaagaa ttatacttac aataacatca attaataaat ggttaatcat ttttgatata
     7981 tcgtaagaat aatgtagttt ttaacaccat ccctggtatc tcaactatct ctataaaaca
     8041 gcgtgacgct gtcgtcctct ggctctatcc cagatgccgt aaaaacgccc tgcattgctg
     8101 gcggtatacc agacagtatg acgaatattg cgatgcccaa gataatcctg gataagtcgc
     8161 gtatctattc ccatattcgc caaagcaaaa ccacacgaat ggcgtaacat gtgcggatga
     8221 atctccagtg acaacccggc attaccaccg gaagtcgaga taatatggta aaactgttgc
     8281 cgagaaagcg gattcccctt acgcgataaa aatacccact cgctctcagc atgcgggtac
     8341 gaagtacgga tactcaacca gttttttaaa gcctgaactt ctttattcaa tagcgggggc
     8401 gttgttgaaa agcctttttt taatcgatgg atatatatac actttgcctt aagatcaata
     8461 tccgaaatcc tcaatcgaca aatttcactc gcccggaaac catgaataaa acaaagcaaa
     8521 gtcagacaat aattacgtgc tgcatgaggc ccggtatttg ctgctttaag gagtgattcg
     8581 atttcactat gggtcaggaa gttccttttt ttgttatcag ccttattctt catcgttttt
     8641 cccttataat tacagacgcg cactagctgt gctgggttag caataatcca acatttttat
     8701 cctgattatg tttataaaag cgaacgtttg cttaataact aacgtaagtg gaccagttct
     8761 tctgagtgaa cttaaatggg gtagcaacag ttaattataa taacgttgcc atacaaacgc
     8821 tatgctcttg catgctatgt acctttatat atttatcaat cggatacgaa tctaataata
     8881 cccctcatca ttaatgcaac taatcttatc tatatatcat gtgatatgtt ctgtaacaag
     8941 taaaatcagc ataaaaaatt cagcaaaatt taatattcgt tcaactagcc gtctcacata
     9001 tgacattatt agtcaactcc atttgccaca aaatggctat ttcatggaac gcagtaattt
     9061 cttagtggct attatcatgc taattcttag gtccctagcg attattcctg gctgattcgt
     9121 cagcttacgc catgagattg ttacattcct ttttatcctg gagatatggg ggaacgcagg
     9181 agatgatttt ttcatctaca ttcgttggta aatatagctt tggtcactac gacttaaacc
     9241 ttcctcaatc acagccattc gccgggcgat acggcaaaca aactaagtga aacatcatcc
     9301 tgaaaacacc aacatcaaca agcctctcca gatcgacttc agaagtgacc agttacaagc
     9361 cacaaaccag gaaacatatt tcatgatgag aattatgctc agcctgatgg cgggtaaaca
     9421 ctgattttca ccaagctcac tgagttagct tgcaaagctt cccttgttta gactccaaat
     9481 aaattattta taaaacaaac atttaaacaa agatctttat tttttgaaga ccaccgcaag
     9541 tgttcgtctg gcttcacatg gcatcttcct cttagaaaaa gatcgacata ttttgtgaca
     9601 cgaattgcaa atctggtttt gttgtatgga ttgcgtgatt tttgatctgg tataacaggt
     9661 ataaaggtgc accaagatag tcaatgagac agggcatctc gcaatctatg gcaaacatca
     9721 cttcagttct ttctcatcgg gtgatgaaaa cgcacttcag tctgaaagga atatgaaaat
     9781 gagatcaaca gacactctat tttatgactc tgggtaaaat ggattgagta agtgatatag
     9841 cttacgaaaa ttcaaatcaa ttaaacatca gaagagattt tatactcagg tatttaatct
     9901 ggatctctgt ttatttaaat aatgtgaaaa gagatttttc acaggagacc ttatacaaaa
     9961 aaatataaaa tacagctacc ggttgccaaa gacactataa gcctggcaaa aaatattaca
    10021 caacataaat gctaattgtt tatgcgggct ttgtattgct ttctgtatcc tacaaatgag
    10081 tgaaatttat gaaaaaggct aaaatacttt ctggcgtatt attactgtgc ttttcgtccc
    10141 cattaatttc tcaggctgcg acactggacg tacgtggtgg atatcgtagt ggaagccacg
    10201 cctatgagac tcgactcaaa gtcagtgagg gatggcaaaa tggatggtgg gcaagcatgg
    10261 aaagtaatac ctggaatacc attcatgata ataaaaagga aaatgccgca ctcaatgatg
    10321 ttcaggttga agttaattac gcgattaaac ttgatgatca atggacggtg cgcccgggaa
    10381 tgttaacgca ttttagcagc aacggcacac gctacggacc ctacgtaaaa ctgtcctggg
    10441 acgcgacaaa agatcttaat tttggcattc gctatcgtta cgactggaaa gcttaccgac
    10501 aacaagactt atccagtgat atgtctcgtg ataacgttca tcgttgggat ggatatgtca
    10561 cttaccatat taatagtgat ttcaccttcg catggcaaac gacgctatac agcaaacaga
    10621 acgattatcg ctatgcaaac cataagaaat gggcgacgga aaatgcattt gttctacaat
    10681 accatatgac gcccgatatt acgccataca tagaatatga ctaccttgac cgtcagggtg
    10741 tttacaacgg cagagataat ttatcggaaa acagttatcg cattggtgtg tcatttaaac
    10801 tgtagtagac aggagacagt cacaatgaat aaaacaataa cggcgcttgc tatcatgatg
    10861 gcttcatttg ccgcaaacgc gtctgtatta ccggaaactc ctgtgccatt taaaagtggt
    10921 accggagcaa ttgataacga cactgtctac attggtttag gtagcgcagg tacggcatgg
    10981 tacaagctgg atacacaggc caaagataaa aaatggacag cgttagctgc attccctggt
    11041 ggaccaagag atcaagcaac ctctgcattt attgatggca atctgtatgt gtttggcggc
    11101 attggcaaaa acagagaggg cttgactcag gtatttaatg acgtacacaa atacaacccc
    11161 aaaaccaata gctgggttaa attgatgtcg cacgcaccga tgggcatggc gggtcatgtg
    11221 acttttgtac acaacggcaa ggcttatgtt actggcggtg ttaaccagaa tatcttcaat
    11281 ggctattttg aagatctcaa cgaggctgga aaagattcaa ccgctgtaga taaaatcaat
    11341 gcctactatt ttgacaaaaa agcagaagat tatttcttca ataagtttct gttgtctttt
    11401 gatccctcaa cacagcaatg gagttacgct ggcgaatcgc cctggtacgg aacggctggt
    11461 gcggcggttg tgaataaagg tgataaaacc tggcttatta atggcgaagc caaacccgga
    11521 ttgcgaacgg atgccgtatt tgaacttgat ttcaccggta ataatttaaa atggaataag
    11581 cttgctcccg tctcatcacc agatggcgtc gctggcggtt ttgcggggat aagcaatgat
    11641 tctcttatat ttgccggagg ggccggattc aaaggttcac gagaaaatta ccagcacgga
    11701 aagaactatg cgcatgaagg cctgaaaaaa tcatatagca ctgatattca tctttggcat
    11761 aacgggaaat gggataaatc gggtgaatta tcgcaaggtc gggcctacgg agtatcattg
    11821 ccctggaata atagtctatt gattattggc ggtgaaactg caggcggcaa agcggtgacg
    11881 gattcagttt tgatctctgt gaaggataat aaagtcacag tacaaaacta acgcttcagg
    11941 gccccagtaa aggggccctg ctatcaattt atcatttcaa ttacgcgaaa attatatgga
    12001 cgcaataata tcgcccgatt attactatgt tcttaccatt gctggtcagt ctaatgctat
    12061 ggcgtatggc gaaggactgc cattaccggc cagggaagat gcgcctcatc ccagaattaa
    12121 acaattagcg agatttgcgc atacgcatcc cggaggcccg tcatgtcact ttaacgacat
    12181 tattccacta actcactgcc cacacgatgt tcaggatatg cagggttatc accatcctct
    12241 ggcaacgaat catcaaacac agtacggcac cgttggccag gcactgcata ttgcacggaa
    12301 attactgtcc tttattcctg ataacgcagg ggttctcatc gttccgtgtt gccgtggcgg
    12361 atcggctttt accgcgggca gcgaagggac atattcagaa cggcacggag ccagccatga
    12421 tgcttgtcgt tggggaacgg atactccgct ataccaggat ttagtcagca gaacgcgagc
    12481 cgcactggca aaaaatccgc agaacaaatt cctcggcgta tgctggatgc aaggcgaatt
    12541 tgacttaatg accagtgact acgcgtcaca ccctcaacac tttaatcata tggttgaagc
    12601 ctttcgtagg gatctaaaac aataccattc tcagcttaat aatattactg acgcgccgtg
    12661 gttttgcggc gataccacct ggtactggaa agaaaatttc cctcatgcgt atgaagctat
    12721 ttatggcaat tatcaaaata atgttttagc taatattatt ttcgtcgact tccagcaaca
    12781 aggtgaaaga ggactgacga acgcgcctga tgaagacccg gacgatttaa tcatgggata
    12841 ttacggttca gcgtaccgct caccggagaa ctggacgacg gcactgcgaa gcagtcattt
    12901 cagcgcggca gcccgtcggg ggattatttc tgacaggttt gtagaagcaa ttttgcagtt
    12961 ttggcgcgga aagtgagcgt tcattaattt gatttattcg catccatcaa cgcttttaac
    13021 tcctccggcg taaacggtgg tttgcgtcgg tgcaacatcg catggcaatt agggcacaca
    13081 agcagcaaaa aaacaaatga gatcaaggta atatatcttc acttgcaggc ttgaacagca
    13141 gaaaatttgc gagcatgatc gcaattattt cactgctagg gatatcaatg gcttccagta
    13201 aatcgctaca gcaggcaatt gccaatataa aaatctggca taagggtgaa cagcgcgcac
    13261 cgcataaacc attgttattg ctatacgtat tagcgggata cctgaatgga catccacgcc
    13321 ttttcgatta tggatcggaa atctacgaac ctctgcacag tttactggaa cgttttgggc
    13381 cacagcgttc acagtaccga cctgatatgc cgttctggcg attacaaggc gacggattct
    13441 ggcaattaca caatgccgag ctttgctcaa cagcagggag cagccgacaa ccgccagtga
    13501 aggaactgaa cgaataccac gttgcaggcg gtttcgatga acagcactac gccctggtaa
    13561 ccggcaataa aaaactcatc aatactctag cgcaacagat actcgaagcg cacttcaccg
    13621 agagtattca ggaagaaatt gctgacgagc tgggttttga tctccagcaa atccgtaaac
    13681 agcgcgaccc gttatttcgc aaaaatgtat tacgcgctta taactaccag tgcgcgatat
    13741 gtggctttaa tatgcgccac gacgacacca ccgtcgcgct ggaagcggcg catataaaat
    13801 ggaaacagca cggcggtcct tgtgaaattc ctaatggcct ggcgctttgc gcaattcacc
    13861 ataaagcctt tgataaagga tcgattggcc tggacgaaaa tatgcgggta ctggtatcag
    13921 atgcagtaaa cggcggaggg agcgttgaga gattattctg ggattttgac gggaaaatta
    13981 ttgcgttgcc gcaggtgcgt aaaaattacc cttatgaggg gtttgtggag tggcatcgga
    14041 atgaggtttt tagggggtga ggttgataac tatcgcagta tgttcaatat tgctgacata
    14101 atcagtgcag gatcttgccg ctagtttaca actcgccttc gatagcggcg caaaacgagt
    14161 tctgttgccg atatcttggg ctatggatat tccaacagtt ccggcagagt tgtttaccaa
    14221 gtttcaggta agtttttact cagaaccggt tgatgcggtt tataaggcgc tggatgtgaa
    14281 ttaacacatt cctcagctta tttacaaaag gattattccc atttgatata atatcagtgc
    14341 caatttactt ttttcaatgg aatatatctc ggctaaataa caataaaact tatcgctaat
    14401 ggattaaata aacaattttc taattcatt
//
LOCUS       DADLFA010000100        14293 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000100 DADLFA010000000
VERSION     DADLFA010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14293
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.100"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..1150
                     /gene="bcsE"
                     /locus_tag="JQH65_002549"
     CDS             <1..1150
                     /gene="bcsE"
                     /locus_tag="JQH65_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HAY6139929.1"
                     /translation="HCSLLVINPGNNNDKQFSLLLEEYRSLFGLASLRFQGDQHLLDI
                     AFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAEIQPRSDEKRILSNVAVLEGAPPLSE
                     HWQLFNNNEVLFNEARTAQAATVVFSLQQNAQIEPLARSIHTLRRQRGSAMKILVREN
                     TASLRATDERLLLACGANMVIPWNAPLSRCLTMIESVQGQKFSRYVPEDITTLLSMTQ
                     PLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGVLVALRPVPGIRVEQALTLCRPNRTG
                     DIMTIGGNRLVLFLSFCRINDLDTALNHIFPLPTGDIFSNRMVWFEDDQISAELVQMR
                     LLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRIPEPMRLLDDAVERSS"
     gene            1147..1338
                     /gene="bcsF"
                     /locus_tag="JQH65_002550"
     CDS             1147..1338
                     /gene="bcsF"
                     /locus_tag="JQH65_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HAY6139930.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            1335..3014
                     /gene="bcsG"
                     /locus_tag="JQH65_002551"
     CDS             1335..3014
                     /gene="bcsG"
                     /locus_tag="JQH65_002551"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HAY6139931.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVETTGGAPVVGDMPAQTTPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTTVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(3101..3208)
                     /locus_tag="JQH65_002552"
     CDS             complement(3101..3208)
                     /locus_tag="JQH65_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HAY6139932.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            3684..4955
                     /locus_tag="JQH65_002553"
     CDS             3684..4955
                     /locus_tag="JQH65_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAY6139933.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            complement(4985..5989)
                     /gene="dppF"
                     /locus_tag="JQH65_002554"
     CDS             complement(4985..5989)
                     /gene="dppF"
                     /locus_tag="JQH65_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HAY6139934.1"
                     /translation="MSTQEATLQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLSGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(5986..6969)
                     /gene="dppD"
                     /locus_tag="JQH65_002555"
     CDS             complement(5986..6969)
                     /gene="dppD"
                     /locus_tag="JQH65_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HAY6139935.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            complement(6980..7882)
                     /gene="dppC"
                     /locus_tag="JQH65_002556"
     CDS             complement(6980..7882)
                     /gene="dppC"
                     /locus_tag="JQH65_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HAY6139936.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDMLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            complement(7892..8911)
                     /gene="dppB"
                     /locus_tag="JQH65_002557"
     CDS             complement(7892..8911)
                     /gene="dppB"
                     /locus_tag="JQH65_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HAY6139937.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPASGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            complement(9219..10559)
                     /locus_tag="JQH65_002558"
     CDS             complement(9219..10559)
                     /locus_tag="JQH65_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001488423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY6139938.1"
                     /translation="MYTFHLRKGVKWHDNKEFKPTRELNADDVVFSFDRQKNAQNPYH
                     KVSGGSYEYFEGMGLPELISEVKKVDDNTVQFVLTRPEAPFLADLAMDFASILSKEYA
                     DAMMKAGTPEKLDLNPIGTGPFQLQQYQKDSRIRYKAFDGYWGTKPQIDTLVFSITPD
                     ASVRYAKLQKNECQVMPYPNPADIARMKQDKSINLMEMPGLNVGYLSYNVQKKPLDDV
                     KVRQALTYAVNKDAIIKAVYQGAGVSAKNLIPPTMWGYNDDVQDYTYDPEKAKALLKE
                     AGLEKGFSIDLWAMPVQRPYNPNARCMAEMIQADWAKVGVQAKIVTYEWGEYLKRAKD
                     GEHQTVMMGWTGDNGDPDNFFATLFSCAASEQGSNYSKWCYKPFEDLIQPARATDDHN
                     KRVELYKQSQVVMHDQAPALIIAHSTVFEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            complement(10569..10799)
                     /locus_tag="JQH65_002559"
     CDS             complement(10569..10799)
                     /locus_tag="JQH65_002559"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6139939.1"
                     /translation="MLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNPQLFTSGTTY
                     DASSVPLYNRLVEFKIGTTEVIPGLAEKWEVS"
     gene            complement(11570..11646)
                     /locus_tag="JQH65_002560"
     tRNA            complement(11570..11646)
                     /locus_tag="JQH65_002560"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11610..11612),aa:Pro,seq:cgg)
     gene            complement(11738..13429)
                     /gene="eptB"
                     /locus_tag="JQH65_002561"
     CDS             complement(11738..13429)
                     /gene="eptB"
                     /locus_tag="JQH65_002561"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HAY6139940.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAKEAAAN"
     gene            complement(13669..14211)
                     /gene="lpfE"
                     /locus_tag="JQH65_002562"
     CDS             complement(13669..14211)
                     /gene="lpfE"
                     /locus_tag="JQH65_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HAY6139941.1"
                     /translation="MKRTILALCLLPLSFSSFAATTDLGPKGTLKFTLKISQGACELV
                     KDSVEVDMGTAVLKKPVRIGTEINPTPFSIGLKNCSEVVRAYVTMDGTPNADDSNLFA
                     LDAGGATGVGLKIKTAAGVQQFPENTTPIPVEFAINGDGAHQLNYIASYVPIRADATI
                     GRADATVDFTVQYSTNNFTS"
BASE COUNT         3393 a         3805 c         3597 g         3498 t
ORIGIN      
        1 ccattgttcg cttttggtaa ttaatcccgg aaataataac gataaacaat tttcattgtt
       61 gcttgaggaa taccgttcac tttttggtct tgccagtttg cgttttcagg gtgaccaaca
      121 tttgctggat attgccttct ggtgcaacga aaaaggggtc agcgcccgtc agcagcttag
      181 cgttcagcaa caaaatggta tctggacatt agttcaaagc gaagaggcgg agatccaacc
      241 acgcagcgac gaaaaacgca ttctgagtaa tgttgctgta ctggaaggtg cgccgccgct
      301 atcggaacac tggcaactgt tcaacaataa cgaagtcctg ttcaatgaag cccgtaccgc
      361 tcaggcggcg acggtggtct tttctttaca gcaaaatgcg caaatcgagc cactggcccg
      421 cagcattcat accctgcgtc gccagcgcgg tagtgcgatg aaaatcctcg tgcgggaaaa
      481 taccgctagc ctgcgcgcca ccgatgaacg tttgttattg gcctgcggtg caaatatggt
      541 tattccgtgg aatgcgccac tctcccgttg tctgacgatg atcgaaagcg tgcaagggca
      601 gaagtttagt cgctatgtgc cggaagatat cactaccttg ctgtcaatga cccagccgct
      661 caaactgcgt ggtttccaga agtgggatgt gttctgtaat gccgtcaaca acatgatgaa
      721 taaccctcta ttacctgccc acggtaaagg cgttctggtt gccctacgtc cggtaccggg
      781 tatccgcgtt gaacaagccc tgacgctgtg tcgccctaac cgtaccggcg atatcatgac
      841 cattggcggt aatcggctgg tgctgtttct ctcattctgt cggattaacg atctggatac
      901 cgcgttgaat catattttcc cattgcctac tggcgacatt ttctcaaacc gtatggtctg
      961 gtttgaagat gatcaaatca gtgccgagct ggtgcagatg cgcctgcttg ccccagaaca
     1021 atggggcatg ccgctgcctt taacgcaaag ttctaaaccg gtcatcaatg ccgagcacga
     1081 tggtcgccac tggcgacgaa taccagaacc catgcgactg ttagatgatg ctgtggagcg
     1141 ctcatcatga tgaccatcag cgatatcatt gaaattattg tcgtttgcgc actgatattt
     1201 ttcccgctgg gctatctggc gcggcactct ttgcgacgca ttcgcgacac cttacgtttg
     1261 ttctttgcta aacctcgtta tgttaaacca gccgggacat tacgccgcac ggaaaaagcc
     1321 agggcaacca aaaaatgact caatttacgc aaaataccgc catgccttct tccctctggc
     1381 aatactggcg cggcctttcc ggctggaact tctattttct ggttaagttc ggcctgttgt
     1441 gggcgggata tcttaacttc catccgctcc tcaatttggt gtttgccgcg tttctgctga
     1501 tgcccattcc gcgctacagc ctgcatcgct tgcgccactg gattgccctg ccgatcggct
     1561 ttgctttgtt ctggcatgac acctggttgc ctggcccgga aagcataatg agccagggtt
     1621 cgcaggtggc ggggttcagt accgattatt taatcgacct tgtcacacgc tttattaact
     1681 ggcagatgat tggggccatt tttgttttat tagtggcctg gttattcctg tcacaatgga
     1741 ttcgcattac cgtttttgtg gttgccatac tgctatggct gaacgtactt accctggcgg
     1801 gaccaagttt ctccttgtgg ccagccggac aaccgacgac cactgtaaca acgacgggtg
     1861 gtaacgcagc ggcaaccgtt gagacgacgg gtggcgcacc ggtagtgggt gatatgcccg
     1921 cacaaactac accgccaaca acggcgaacc ttaacgcctg gctgaataat ttctataacg
     1981 cggaggcgaa acgtaaatcg accttcccgt cttcgctgcc cgctgatgct cagccatttg
     2041 aactactggt gattaacatc tgttcgcttt cctggtcgga tatagaagcc gccgggttga
     2101 tgtcgcatcc actgtggtcg catttcgata ttgagttcaa gaactttaac tccgccacct
     2161 cctacagtgg cccggcggcg atccgtttac tgcgcgccag ctgcgggcag acttcgcaca
     2221 ctaatctgta tcaaccggca aataacgact gctatctgtt tgataacctt tcgaaactgg
     2281 gctttaccca gcacctgatg atgggacata acggccagtt cggcggtttt ttgaaagaag
     2341 ttcgcgaaaa tggcggcatg cagactgaat tgatggatca aacaaatctg ccggttattt
     2401 tgctgggctt tgatggttcg ccggtttatg acgataccac cgtgcttaac cgctggctgg
     2461 acgttaccga aaaagataaa aacagccgta gtgccacgtt ctacaacacg cttccactgc
     2521 atgacggcaa ccattatccg ggggtcagca aaacagcgga ttacaaagcg cgggcgcaga
     2581 aattctttga tgaactggac gccttcttta ctgaactgga gaaatcgggt cgtaaagtga
     2641 tggtggtcgt ggtgccggaa cacggcggcg cgctgaaggg cgacagaatg caggtatctg
     2701 gcctacgtga tatccctagc ccgtctatca cagacgtccc cgttggggtg aaattcttcg
     2761 gcatgaaggc accacatcag ggggcaccga ttgtcatcga ccaaccgagc agcttcctgg
     2821 ctatctccga tctggtggtt cgcgttcttg atggcaagat tttcaccgaa gacaatgttg
     2881 actggaaaaa actcaccagt gggttgccac aaacagcacc ggtctccgag aactcaaatg
     2941 cagtagttat tcaataccag gataaaccgt acgttcgcct gaacggcggc gactgggtgc
     3001 cttacccgca gtaagcacga aaaaggccgc agagtttaac ctctgcggcc tgatccgggc
     3061 gcaaattgcc attacggcag cctgacgccc gcatgacacg ttacttccgc ttattcagcc
     3121 agttcacgat catactggca agaatgccag caatgaccgg agccgctaaa tcatgccaga
     3181 aggccatgcc cagttctgcg agcgtcatat agccgcctgt gttgtaatgc caacgtttcg
     3241 cggctattct tgagttgttt gggttcaaga ttagcccccg tgatgttgtc aggtgcatac
     3301 ctgcaacgtg cgggggtttt ctctctccag caaccaatgc caccagggat aaagcccccg
     3361 caacattgcg cctcaccgga accctttcgg cttgccgctg attttacgat tgctttttac
     3421 tgtcagcacc cggcaatctt cctcattgcg cgatgccttc cagcttaatt tctttatttg
     3481 caatagttgc agttcatatc cgggctgact ccagactggt agtgaaatgc gcaattttgt
     3541 agaattgcgc ctcctcgcga cctccccagt gaatctgttg cctcattttt tacatactgc
     3601 gtattcgact tctccacctg ttgcgcaaga gaaactgggt ttattcattt ttgcgaggcc
     3661 gacttctttc tggacaggac tttatgcagc acaacacact atcgaaacac aatcagaaat
     3721 tgccgtttac acgctacgac ttcggctggg ttttattatg cataggcatg gcgattggtg
     3781 ccggaaccgt gctgatgcca gtacaaattg gcttgaaggg aatttgggta tttattaccg
     3841 cagcgatcat tgcttatcct gccacctggg tagtgcagga catttattta aaaacccttt
     3901 ctgaaagcga ttcctgtaat gactacaccg atattatcag tcattacctg gggaagaact
     3961 ggggaatttt cctcggggtt atctactttt tgatgattat ccacgggatt tttatctact
     4021 ctctctccgt ggttttcgac agcgcctcgt acctgaaaac cttcggttta accgatgccg
     4081 atctttcaca atctctactt tataaagtcg ctattttcgc cgtactggta gcgattgcgt
     4141 ctggtggtga acgattactg tttaagattt ccgggccaat ggtggtggtc aaagtaggga
     4201 ttattgtcgt gttcggtttt gcgatgatcc cgcactggaa tttcgccaat ataaccgcct
     4261 tcccgcaagc ctccgtcttt ttccgcgatg tcctgcttac aattccattt tgcttctttt
     4321 ctgcggtatt tattcaggta cttaacccaa tgaatattgc ctatcgtaaa cgggaagcag
     4381 ataaagtact ggcaacccgg ctcgcgctgc gtacccaccg aattagttat atcacgctca
     4441 tcgcggtgat cctgtttttt gccttttcgt ttaccttctc aattagccac gaagaagccg
     4501 tttctgcctt tgaacaaaat atctcggcac tggcactggc cgcgcaggtg atccctggac
     4561 atatcattca tatcacctct acggtgctga atatctttgc cgtactgacc gcattctttg
     4621 gcatttatct cggtttccac gaggctatta aaggcattat tctcaatctg ttaagccgaa
     4681 ttattgatac caagaaaatt aactcacgca tgctgactct ggcgatctgc gcttttatcg
     4741 tcattacgtt gacgatttgg gtttcgtttc gtgtatcggt gctggtgttc tttcagttgg
     4801 gaagcccgtt atatggcatt gtgtcgtgcc tcattccgtt tttcctgatc tataaagtcg
     4861 cacaactgga aaaacttcgc ggatttaaag cctggctaat tctgctttac ggcattttgc
     4921 tatgcttgtc gccactgttg aagctgattg agtaaaccgg agcgcatggc cccggttttg
     4981 tgagttaacg ctgcggattt tcatcctgat caacagcaaa acaagctacc agttgaccgc
     5041 cgtagtcttt tagctgcggc tgcaactggg tgcaggggcc gaagcgccga cgacagcggg
     5101 cgttgaaggc gcaacccggc ggtggattca gtgggcttgg tagttcaccg ctgagcttga
     5161 tgcgctcgcg gcgatcgtcc gggttcaggc gcggcgtcgc ggaaagtagc gcctgagtgt
     5221 acggatggcg cgggttattg aagatttggt ctttcgttcc cttctccacg cagcggccca
     5281 ggtacatcac catcacttca tcagcaatgt gctccaccac cgacaggtcg tgggagataa
     5341 agacataaga cagccccaac tcctgctgca aatccatcat cagattcagc acctgcgcgc
     5401 gcactgaaac atccagcgcg gaaaccggtt catcggcaat caccacatcc gggtcgagca
     5461 tcagaccacg ggcgatggcg atacgctgac gctgaccgcc ggagaacata tgcggatagc
     5521 ggtcatagtg ctcggttttc aggccgactt tcgccatcat cgacagggct ttttcccgac
     5581 gctgttcttt gcttaagctg gtgttgatca gcagcggctc ttcaagaatt tgcccgactt
     5641 ttttacgcgg attcagcgaa ccgtaagggt tctggaagac gatctggatt ttctgccgac
     5701 gcagcttctg cgcctgcgga tcgtgcttaa gcagatcctg cccctggtaa tacagctcgc
     5761 caccggtggg cgtttcaatc atcgtcagca accgaccgag ggtcgattta ccgcagccag
     5821 attcgcccac tactgccagc gttttgccac gttcaaggtt aaacgaaacg ccatccagcg
     5881 ctttaaccag acgttccggc gcgaacatgc ctttcttcac cggataatgt tttttcaggt
     5941 cgatagcctg caacagcggt tgttgcaggg tggcctcttg cgtactcata gtgtcggcct
     6001 cccggcatca tcaagtgggt aatggcattt ggactgacgc ccgtcagcga gcatattcag
     6061 cgccggttct tcagcgcgac atctgtcagt ggcatagggg cagcgcgggt taagcaggca
     6121 gccgttcggg cggtcgtact tgccgggaac gacacctggc aacgacgcca gacgttcttt
     6181 gtcctgagca aattctggca gcgcacgcag caatgcctga gtatacgggt gacgcggcgc
     6241 atggaagatg gcgtgcgcat cgcccgtttc caccacctgg cctgcataca tcacaatgat
     6301 tttatgcgca gcttccgcca ccagcgccag gtcatgggta atcaacacca gcgccatgtt
     6361 ctctttctgc tgtagctcca gcagtagttc gatgatttgc gcctgaatgg tcacgtccag
     6421 cgcggtggtc ggctcatcgg caatcagcag ttttggtcga caggcaatcg ccatggcgat
     6481 catcacgcgc tggctcatgc cgccggaaag ctgatgcggg taaacatcca gacgcgaagc
     6541 cggatcggga ataccgacct gattcagcag gtcaatcgcc cgctgacggc gggtactttt
     6601 gttgccgccc tgatgcacct taatcgcttc catgatctgg aaacccacgg tgtagcacgg
     6661 gttaaggctg gtcatcgggt cctggaagat catcgccact tcggcaccca ccaggttgcg
     6721 gcgctctttt tctgatatac gctgcaaatc ctgaccgtta aacaccagtt tttccgccat
     6781 tacgcggccc ggataatcaa tcagccccat aatcgccagg gaactgaccg acttaccgga
     6841 gccggactca cccacaatcc caaccacttc gccctgtttt acgctgtagc tgatgcggtc
     6901 tacggcgcgg aacggtgcgc tttcgtcgcc gaaatgcacc gataatttat ctacatttaa
     6961 taacgccatc tcgaacctct tactgcttca gtttgggatc gagcgcgtca cgcagaccgt
     7021 cacccatcag gttaaatgcc agcaccgtca gcaggatcgc cagacccggg aaggtcacga
     7081 cccaccaggc gctttgcgcg aactgcaaca cgtcggagag catggtgccc cactccggcg
     7141 ttggcggctg tgcccccatg ccgaggaaac caagagcagc catatcgaga atggcgttag
     7201 agaaaccgag cgacgcctga acaatcagcg gcgcaaggca gttcgggaag atgttaataa
     7261 acatctgacg catcgccccg gcacccgcca cgcgagacgc ggtgacgtaa tcgcggttaa
     7321 cttccaccag cacggcggcg cgggttaagc gcacatagtg cggcaaggca acgaaggtca
     7381 gtgccagcgc ggcgttacca atcgacgggc cgaaaattgc caccagcacc agcgccagca
     7441 gcagacttgg cagcgccagc atgatatcga ccacgcgcat aatgatgtta tcgactaggc
     7501 cgccaaagta accggcgatc aggccgagaa taacgcccat aatcagcgat aacacaacta
     7561 ccagacagcc aaccagcagc gacaggcgcg caccgtacat caggcgcgac agcatatcac
     7621 ggcctacgtc atcggtgccc agcaagtgcg ccatgctgcc gccttcctgc caggctggcg
     7681 gggcgagcag tgcatcgcgg aactgttccg ccgggttata gggtgcaatc cagttggcaa
     7741 agatcgcgat gaacagcacg atgacgacgt aaaccagccc gacgaccgcg cctttgttgc
     7801 gtttaaaata gtgccagaac tcctgtaacg gggtcatcgg caccggtgcg ctaatcactt
     7861 tattttcagt aacctgtgac atgatgtccc cttacttctt atgacgaata cgcgggttca
     7921 ccacgccgta cagcagatcg accagcaagt tgacgaggat aatcatcgtc gccaccagca
     7981 atacgccgcc ctgcactacc ggatagtcgc ggcgttgcag tgcgtcaatc aaccagcgtc
     8041 ccagaccggg ccacgagaag atggtttcgg tcagaatcgc ccccgccagc aatgttccca
     8101 cctgcaggcc gataacggtc accaccggca gcatcgcgtt acgcagcgca tggacgataa
     8161 tcacccgcat gcgggttagc cctttggcgc gcgcggtgcg gatgtaatcc tcgcccagca
     8221 cttccagcat cgaggagcgt gtcatacgca caatgaccgc cagcggaata gtacccagca
     8281 caatggcagg caagatcata tgggcgacgg catcgataaa gttgccgtct tcaccccaga
     8341 tggcggtgtc gattagcata aaaccggtta acggattgga gtcatcgagg aacaccatat
     8401 cgctcacgcg accggaggcg ggcgtcaggt tccagtgcac cgaaaccagc atgatcagca
     8461 tcatgcccca ccagaagatc ggcatcgagt agccggtcag cgccaggcca actgcagtgt
     8521 gatcgaaaat ggaaccgcgt ttaaccgcag ccagcacacc aaccggaata ccgactgccg
     8581 tagcaaaaat catcgcgcag acgccaagtt ccagcgtggc ctgaaagcgc ggcacgaact
     8641 cttcccatac tggaattcgg cttttcattg aaatgcctag atcgccgtgc attacgcccc
     8701 aaatgtaatg gagatactgc tgccacatcg gtttatctaa gccgagttca gccagcagct
     8761 gcgcgtgacg ctcaggggag atcccacgtt cgcctgccat gatcatcacc ggatcgcccg
     8821 ggatcatgtg gacaaaggca aatgtgagaa gggtaatacc gataaacgtg gggatgacga
     8881 gtcccaaacg tcggagaata aactgcaaca taacccggat tctctatagt gacccgcagc
     8941 ctggaactgc gtgtctgtat tgctcacaaa tcaattcccg cccttgtcgg acagagaatg
     9001 aagtattgct gccggatggc gctgcacgca gaggcacagc gccaccgggt atacagattg
     9061 ttgacaaagt gcgctttgtt tatgccggat gcggcgttaa cgccttatcc ggcctacaaa
     9121 atcgtgtaaa ttcaatacat tgcagaattt tcgtaggcct gataagcgga gcacatcagg
     9181 cagttttgcc tttaccatca gtcttgtatg gcttttaatt attcgataga gacattttcg
     9241 aagtgatgtt tgcctaatgg atcaaccaca tagcctttga cttctttgcg taccggttca
     9301 aacacggtgg agtgagcgat gatcagtgcc ggagcctgat cgtgcatcac cacctgcgat
     9361 tgcttgtaca gttcaacgcg tttgttatgg tcgtcggtag cacgcgccgg ttgaatcaga
     9421 tcttcaaacg gtttgtagca ccattttgag tagttagaac cttgttcaga ggcggcgcag
     9481 ctgaacaggg tggcgaagaa gttatccggg tccccgttat cgccagtcca gcccatcatt
     9541 accgtctgat gttcgccatc tttcgcacgc ttgaggtact caccccattc gtaggtgacg
     9601 attttggcct gcacgcccac tttcgcccag tctgcctgaa tcatctccgc catgcagcga
     9661 gcgttcgggt tatacggacg ttgtaccggc atcgcccaca ggtcgatgga gaaacctttt
     9721 tccagacccg cttctttcag caaggctttc gctttttcag gatcgtaggt gtaatcctga
     9781 acgtcgtcgt tatagcccca catggttggc gggatcaggt ttttcgctga tacacccgcg
     9841 ccctgataaa ccgctttgat gatcgcgtct ttgttcaccg cgtaggtcag tgcctggcga
     9901 actttcacgt catccagcgg ttttttctgc acgttatacg agaggtagcc gacgttcagc
     9961 cccggcattt ccatcaggtt gatggattta tcctgcttca tgcgagcgat atctgccggg
    10021 ttcgggtacg gcatcacctg gcattcattc ttctgcaatt tcgcgtaacg cacggaagca
    10081 tcaggggtaa tagagaagac cagcgtatcg atctgcggtt tggtgcccca gtagccatca
    10141 aacgctttgt agcggatacg ggaatctttt tggtactgct gtaactggaa cggaccggtt
    10201 ccgattgggt tgaggtccag tttttccggt gtaccggctt tcatcatcgc atcagcatat
    10261 tcttttgaca gaatagaggc gaagtccatt gccagatcag cgaggaacgg cgcttccggg
    10321 cgagtcagta cgaactgaac tgtgttgtcg tccacctttt tcacttcact gatcagctcc
    10381 ggcaagccca tgccttcgaa gtattcgtag ctgccgccag agactttatg gtacgggttt
    10441 tgcgcgtttt tctgacggtc gaacgagaac accacgtcat cggcgttcag ttcacgcgtc
    10501 ggtttgaatt ctttattgtc gtgccacttc acacctttac gcagatggaa ggtatagatt
    10561 ttaccgtctt agctgacttc ccacttttca gcgaggcccg gtatcacttc agtggtgccg
    10621 attttaaatt caaccaggcg gttataaagc ggtacggaag aggcgtcata ggtggtgccg
    10681 gaggtaaaca gctgcgggtt aaacccttcc ggagagcctt ctgagcaata aaccagagtt
    10741 ttagcctgaa cacttgctgc gacggtcata gccaccaggc tgagaccaag cttcagcatc
    10801 cctgactttt tcaaggaaat acgcattatt ctgctccaat tgtgatgttt gttgttttaa
    10861 ccctttgcag tgggttgtcg ctacctgacc ttttttgttt tttgcccggt cgggtcaacg
    10921 ttatgaggtg gggatgccgt actaattaac atcagtgtaa cagtgcggat cctccataaa
    10981 atgcccctcg tgacctacaa tctgtcaaca gaatgtgaaa acgtcaatac agccaaccgg
    11041 gatttacacc aacggtgaga acccacaaac aaagattaaa aaacttcaaa cagctatttg
    11101 cagcagagaa atttgtgcta ctccaacata accagaacaa tcagcttaat atttagcaac
    11161 atttggtgat taagttttat gcatttaatg aaaaaaatct gaggaaaagg tggatatctg
    11221 agcgattaat accatgaacg ctaaaacgca cagcggaaaa tgccagcgcc agccataagt
    11281 aacgcaaaaa aagatttaag caaatataaa aaaagacaat ggtttctgtc acaaatccgt
    11341 taatggatag tgagatatgg ggcgcaaagt tggcaggaaa atgtgtggtg tgttggtcga
    11401 ataaggcggt cgcgccgcat ccgacaataa gtgcggtgcc gttgcctgat gcgacgctgg
    11461 cgcgtcttat caggcctacg tcgagtcgaa cctcccagcc cccggaggtt ctcatcctca
    11521 tgggcttcaa atgcaaaaaa gcctgctcgc tgagcaggct tttcgaattt ggtcggtgat
    11581 agaggattcg aacctccgac cccttcgtcc cgaacgaagt gcgctaccag gctgcgccaa
    11641 tcaccgaatg cggggcgcat cttactgcgc agatacgccc tcgtcaatcc cttaatagca
    11701 aaatgccttt tgatcggcga gaaagtcagc aggccgctta gttagccgct gcctcttttg
    11761 cctgcgggat gtgacaccag ttgttgtttt cgttaattcc accatccggt gaagtatagc
    11821 caagacaacc catgatggta tcgtacagct ctacgtgacg gcgtggcact ttcatgtcgg
    11881 cttctttttt cagctgcgca aatgcctgcg cattggccgg attttccaga tatttatctg
    11941 acatccagac catcatcggt acgcggaact gctccggcgg tgccaattca cgcggcgtgc
    12001 cgtgcaggtg ctcgcgttca ttaattgact caccgtggtc agctgcgtag aacacaatcg
    12061 ctttcttatc gcgaacctga tcaatcacgc tggagataaa gtgatccaca taggtcaccg
    12121 agttgtcata ggagttgatc atctgcgctt tggtacagcc gctatcaaca ccaatacatt
    12181 ccggcttcca ctgcgcgaag ctacgcggat aacgctgggt gtagttaaaa tgcgaacctt
    12241 tggtatgcag aatgatcaga tgcttaccat ccgggttgcg ccctagcgat tgctgcattt
    12301 cgtctaccag caacatatca tctaccggct tgccacgatt acgtggctcc gcaccaatct
    12361 gctcacgata agcaatgttg tccgccatcg tgttgctgta gaaccacatt tcgctctgca
    12421 tagcgtagag gtcagaactg aatcctaact gcttcagaac cgcgaaaatg ttctgttctt
    12481 ttaatgtgcg ctgcggatta tcttccgcgc ccccctgacg tacaaacatg caacgcagtg
    12541 agagtttggt tgcggtatca caggagtaac cacggaacgc cgccagattt ttctcctggg
    12601 ccagtttcgg cgtggtatta cgctcatagc cgaaaatacc catatggtcc caacgcgtgg
    12661 tttcaccgat gataaacacg acataagtat catcaacgtt ttgtggagcc tggtatgtaa
    12721 acttcttcgc cggattaagc aatgaattat tatcggaaga ttcatcgacc cgcgcccagg
    12781 catacagccc caacgcagaa agccagtttg atggcagata agagttcgct acgacaccgc
    12841 cataactcgg caaatcaacg ccggtcgccc tctccacttt cttctgctgg atatccagca
    12901 aacgaatcgg tgcccaaacc ataatacccg ccagtacgac gaccgccagg ctgcgaatac
    12961 gctgccctgg ggttcgcagt tgtcggagca aggtgtagcg acagcggtta ttccagataa
    13021 ggataagagg caatgcacta acggcgatta accagagaat aaagttcaga ccaacaactt
    13081 cttttgacag gtcgatatcg gtggtcatga cggaagcgat gatgccataa ccaatgacca
    13141 cattaaggaa ggtcatgtaa tagctggcac ctgcggaaaa gagcaccacc agcgacgcca
    13201 gaatacgcca gctgcggcgg ccaaacagcg atagaagacg tagtaaaaag aaggtcacca
    13261 gtacggtggc ggccagttca acaacagcag aaacgccttt ccagacggta aaatcgtgcg
    13321 catagctgcc gaagcggcga taaaaaaccg cgccattcat aaaaaggcca atatagattg
    13381 caagcaaaaa gctcagcttc tgctgtgtaa tcgatttgat gtatctcatg caaacaaacc
    13441 ctggacaaac aggtgataac accctgctta gcggcttttc aggcccggca ggggaaaagt
    13501 gcggaggtag tcagataagt aaaatctaca cgcgcgaata agtagaccac agcaaagcga
    13561 aaaagtgtgg ggaaagagtg tgcatgaagc acaaatttac aaaaatgcaa gtttgaaaag
    13621 agactttagc gttacaaaaa gcagtaaaaa gggccagtta actggccctt aagatgtgaa
    13681 attattcgta ctgtactgta cagtaaagtc gaccgttgca tctgcgcgac caatagttgc
    13741 atcggcgcga ataggcacgt agctggcgat atagttcagt tgatgtgcgc catcaccgtt
    13801 aattgcaaac tcaaccggta tcggcgttgt attttccggg aactgttgta ctccagcagc
    13861 ggttttgatt ttcagcccaa cgcctgtagc gccacccgca tccagcgcaa aaagatttga
    13921 gtcgtctgca ttgggtgtac cgtccatagt cacgtaggct ctgaccactt cgctacagtt
    13981 tttcaggccg atggaaaaag gagtgggatt aatttccgtt ccgattctta ccggcttttt
    14041 gagaaccgcg gtgcccatgt caacctcaac gctgtctttc accagttcgc aagcaccctg
    14101 gctgatttta agggtgaatt tcaatgttcc tttcggccct aaatccgtgg ttgccgcgaa
    14161 tgacgaaaag gaaagaggca gcaggcataa cgcaagtatt gttcgcttca tgactgttac
    14221 tccagttcga catgtatggt agcaataccg ctataatccc cggcagcggg taattttccg
    14281 gtagtgctaa cgg
//
LOCUS       DADLFA010000101        14221 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000101 DADLFA010000000
VERSION     DADLFA010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14221
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.101"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            505..1329
                     /gene="gadX"
                     /locus_tag="JQH65_002563"
     CDS             505..1329
                     /gene="gadX"
                     /locus_tag="JQH65_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HAY6139942.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            1697..2425
                     /gene="gadW"
                     /locus_tag="JQH65_002564"
     CDS             1697..2425
                     /gene="gadW"
                     /locus_tag="JQH65_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HAY6139943.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSLGTFS"
     gene            complement(2788..5901)
                     /locus_tag="JQH65_002565"
     CDS             complement(2788..5901)
                     /locus_tag="JQH65_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HAY6139944.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIGAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5926..7083)
                     /gene="mdtE"
                     /locus_tag="JQH65_002566"
     CDS             complement(5926..7083)
                     /gene="mdtE"
                     /locus_tag="JQH65_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HAY6139945.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            7143..7421
                     /locus_tag="JQH65_002567"
     CDS             7143..7421
                     /locus_tag="JQH65_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6139946.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(7422..7949)
                     /gene="gadE"
                     /locus_tag="JQH65_002568"
     CDS             complement(7422..7949)
                     /gene="gadE"
                     /locus_tag="JQH65_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HAY6139947.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITLTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(8748..9320)
                     /gene="hdeD"
                     /locus_tag="JQH65_002569"
     CDS             complement(8748..9320)
                     /gene="hdeD"
                     /locus_tag="JQH65_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HAY6139948.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            9575..9907
                     /gene="hdeA"
                     /locus_tag="JQH65_002570"
     CDS             9575..9907
                     /gene="hdeA"
                     /locus_tag="JQH65_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HAY6139949.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            10023..10349
                     /gene="hdeB"
                     /locus_tag="JQH65_002571"
     CDS             10023..10349
                     /gene="hdeB"
                     /locus_tag="JQH65_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HAY6139950.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            10413..11060
                     /locus_tag="JQH65_002572"
                     /pseudo
     CDS             10413..11060
                     /locus_tag="JQH65_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(11102..11632)
                     /locus_tag="JQH65_002573"
     CDS             complement(11102..11632)
                     /locus_tag="JQH65_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY6139951.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDGMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEAAVSLSRKQHQVLSCIANQMTTEDILEKLKITLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(11788..12354)
                     /gene="slp"
                     /locus_tag="JQH65_002574"
     CDS             complement(11788..12354)
                     /gene="slp"
                     /locus_tag="JQH65_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HAY6139952.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(12602..13825)
                     /locus_tag="JQH65_002575"
     CDS             complement(12602..13825)
                     /locus_tag="JQH65_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6139953.1"
                     /translation="MSIDFTPGIINTYHGNIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQINNIRYEKNKSAECRIIVVANPKPDKAIITKISVEEGIPITFSV
                     QTMFSDTNFIAEQRADLPTNIKDIQSLYQKMTKLYIEHSENKNRMKVFAGTNFIDFNM
                     TGQNLSGFVLTLSRFYFEDLLNINFTDANLGDTIFYIKNTLPPKLYKDGQYLDKQIEG
                     LFSTLLTINDNFLRAKAEIASTIIKFLEARITNLSYNDILKYQQEFQKQCYKQVKAST
                     TLSRYNKIQTWAEMSEYQFEVFQFETLNPKKMSHTPYLKRPLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
BASE COUNT         3946 a         3020 c         3154 g         4101 t
ORIGIN      
        1 attatgtgtc gtcgcggctt cgaaatggac tttgctgaac tgttgctgga agactacaaa
       61 gcctccctga aatatctcag cgatcacccg aaactgcagg gtattgccca gcagaacagc
      121 tttaaacaca cctgataaca taacgttgta aaaaccgaat gcccagcctt taaaaaaaca
      181 gctgggcatt cggttgctta ttaatcgcaa taatatattg gctgtctatt catcgtgttg
      241 ataagatata tgttatatga atgtttatta aatgtagcga ttgcctttaa tgtcacgaaa
      301 agggattatt tgcttgctat taatttcact gcgttcttaa acgcgttttt gtattataaa
      361 tccagtcatc ctgcccgtag aacatcacac attatcatcc tgttctcccg cttcgtttaa
      421 atttatttat caatcaattt gacttaagag ggcggcgtgc tacattaatt aacagtaata
      481 tgtttatgta atattaagtc aactatgcaa tcattacatg ggaattgtct aattgcgtac
      541 gcaagacata aatatattct caccatggtt aatggtgaat atcgctattt taatggtggt
      601 gacctggttt ttgcggatgc aagccaaatt cgagtagata agtgtgttga aaattttgta
      661 ttagtatcaa gggatacgct ttcattattt ctgccgatgc tcaaggagga ggcattaaat
      721 cttcatgcac ataaaaaaat ttcttcatta ctcgttcatc actgtagcag agatattcct
      781 gtttttcagg aagttgcgca actatcgcag aataagaatc ttcgctatgc agaaatgcta
      841 cgtaaaagag cattaatctt tgcgttgtta tctgtttttc ttgaggatga gcactttata
      901 ccgctgcttt tgaacgtttt gcaaccgaac atgcgaacaa gagtttgtac ggttatcaat
      961 aataatatcg cccatgagtg gacactagcc cgaatcgcca gcgagctgtt gatgagtcca
     1021 agcctgttaa agaaaaaatt gcgcgaagaa gagacatcat attcacagtt gcttactgag
     1081 tgtagaatgc aacgtgcttt gcaacttatt gttatacatg gcttttcaat taagcgagtc
     1141 gcagtgtcct gtggatatca cagcgtgtcg tatttcattt acgtctttcg aaattattat
     1201 gggatgacgc ccacagagta tcaggagcga tcggcgcagg gattgccgaa ccgtgactcg
     1261 gcggcaagta ttgttgcgca aggaaatttt tacggcactg accgttctgc ggaaggaata
     1321 agattataga attttactca gacataaaaa aaacctggca taggggaccg ggaagaggat
     1381 agtctgccgt ctccagacta ataaaccgct ataacactcc ctgttggcac gggaaacttt
     1441 gtgctctcag taagttaaat ataactttta ctggaaataa gatcagccat ttttttataa
     1501 acataagcta tacgctgtgc gaaaatatat tcgtgctgca tttacttatt atcaattaac
     1561 tgttatgcaa aactactttg tggataaatt ttggtcctgc caaatctggc agtttttgcg
     1621 ctaagaaaca gtctggcaac atttcattag tatactgaaa ttgaaataat cgcagtatga
     1681 aatataaggg ataatcatga ctcatgtctg ctcggtgatc ctcattcgtc gttcattcga
     1741 tatttatcat gaacagcata aaatatcgct gcataacgag agtatcgtgc tgctggagaa
     1801 aaatttggca gacgattttg cgttttgttc accggatacg cgacgactgg atatcgatga
     1861 gctgacagtt tgccattact tacaaaatat tcgtcagcta ccacgcaatt tagggttaca
     1921 tagcaaagac cgtctattaa ttaaccagtc accccccatg ccgctggtga cggcgatttt
     1981 tgatagcttc aatgaatccg gggtaaattc accgatactg agcaatatgc tctacctttc
     2041 ctgtttatcg atgttttctc ataagaaaga actgatcccc ttacttttca atagcatcag
     2101 cactgtttca ggaaaagttg aacgccttat tagctttgat atcgccaaac gttggtatct
     2161 gcgcgatatc gcggaaagaa tgtataccag cgagagtctc atcaaaaaaa agttgcagga
     2221 tgaaaatacc tgtttcagta aaatattact cgcttccagg atgtcgatgg ccagacgatt
     2281 actcgagtta cgtcaaattc ctctgcatac tattgcagaa aaatgtggct atagcagtac
     2341 gtcgtacttt ataaacacat ttcgacaata ttatggtgta acgccacatc agtttgcgca
     2401 acattcgcta ggtacctttt cctgacatat tttgcatttg aatattggtc aggatctcac
     2461 acctgcttca tgtgaaactc ttccctgatg atttctgccg ggctaccggc tagttctctt
     2521 tcgcagtttg ccaggcggta taggtatttt caggaggaaa agactcggca tgtttgggat
     2581 tattaagctg acaattcata cgattacggg aatgtgggtc agtattgtgc tgttcaaatt
     2641 gatgacgaat ggctggagtg gtttctactt tcagtgctgt gtactaagcc tggtattcct
     2701 gacggttagc tggttgttga gtggtgaatg gttagcagga aagagtaagg ctgaaccttc
     2761 acgttcaacc ttactctcat ttacacgtta cgctttttta aagcgggcaa agagatgttc
     2821 cactacaacg aaaaagaccg gaacgaagta aattgccagc actgttgcgg caaacatccc
     2881 gcccatcacg ccggtaccta ccgcgttttg cgcgccagaa ccggcaccat gactgataac
     2941 cagcggcagc acgccgagaa taaaggccag agaggtcatc aggattgggc gtaaacgcat
     3001 ccgcgccgct tcgatgattg cccctatcgg cgttttccct tctttctgca tcatctcaac
     3061 ggcaaattcg acaatcagga tggcgttctt ggcggaaagc ccgatggtgg tcagcaaacc
     3121 aacctggaag tagacgtcat tacttaagcc gcgcagatcg gtggccagta atgcgccaac
     3181 gacgcctaac ggaacaacca acatcaccga gaacggaatt gaccagctct catagagtgc
     3241 ggcgagggcg aggaacacca cgaccagtga aatggcatac agtgcaggag cctggtttga
     3301 ggataacgct tcctgatacg acagtccggt ccatgagtag ccgacgcctg ccggaagttt
     3361 agcgaccagg tctgccataa atttcatagc gtcaccggta cttttcccgg ccgccgcttc
     3421 acctaaaatc tccattgacg ggatgccgtt gtagcgttcc aatcgcggtg aaccataggt
     3481 ccattcggta gacgagtagg cagaaagcgg tgccatcgtg ccagaggcgt tgcgtacata
     3541 ccattggttg atgttatccg gcaacatacg gaacggcgtg cctgcctgga catacacttt
     3601 tttcacccgc ccctggttga ggaagtcgtt cacgtagctg ctgccgaagg cggtggaaat
     3661 tgtctggttg atatcagaca gcgccacacc cattgcctca gctttcgcgg cgttgacgtt
     3721 cactttgaac atcggcgtat cttccaggcc gttcgggcgt accccggtga cctggtcagg
     3781 cgattgcgct gccagtgata acagctcgtt tcgtgcctgg gttagttttt cgtgccccag
     3841 gttaccgttg tccagcagtt ccatatcaaa acctgacgcg gtacccagtt cagccaccgc
     3901 gggtaagttg aacgggaaga cgacggcttt attgatactg cttaacgcaa tcattgcccg
     3961 ctgaatgatc gcggtaaccg agttttcctc accgacacgt tcagaccacg gcttgagact
     4021 gataaacgcc aggccgttgt tttgcccctg gccgctgaag ccaaagccgc caacggtaaa
     4081 caccgactga acattatctt tctcttttgt aagataataa tccgtcacct gttgcagcac
     4141 tttcgtggtg ttaaccatgg tggcaccgga aggtaactgc gcggtggtca taaatacccc
     4201 ctgatcctct tctggtaaga aagaggtcgg tgtacgcagg aacagcaccg ccatcccggc
     4261 gcaaatcagc aggtagacca ccatgtagcg accggtacaa cgcaacagcg agcgggtgct
     4321 atcggtatag tgctgagttg atttttcaaa cagcgtgttg aagcgtgcga acagggcgtt
     4381 aggtttgtga ccgccttccg gcgcggcttt cagaatggtg gcgcacaggg caggggtcag
     4441 gctcattgcc acaaatactg aaagcagcat ggaggagatc agcgtgatgg agaactggcg
     4501 gtagatctcc ccggttgcgc cgctcataaa ggccatcggc ataaacactg cggaaagaac
     4561 aacagcaata ccgaccagcg cacgttggat ctgccccatc gatttatgcg tcgcttcctt
     4621 cggtggtagc ttatcttccg caatgacacg ctcgacgttc tccaccacca cgatggcgtc
     4681 atccaccagt aaccctatcg ccagcaccat cccgaacata gttaacgtgt tgatggtgaa
     4741 accgaccgcc gacaagatcg caaacgtccc gagaataacc accggtacgg caatcgtcgg
     4801 gatgattgtg gcacggaaat tctgcaaaaa cagatacatg accaggaaga cgaggatgat
     4861 agcctcaacc agtgttttaa aaacttcctg aatagaaatt tcgataaacg gcgtggtgtc
     4921 gtaaggataa accgtcttca gacttgcggg gaaataggct gataagcggt tcagctcctc
     4981 tttgactgcc cgcgaggtat ccagggcgtt tgctccggta gccagtttga tggcgatccc
     5041 ggcagcaggt ttgccgttat agcgcgccac ggtggaataa tcttccgctc caagttcgac
     5101 gcgggcgaca tcacgcagca gcacttgcga accatcttgc tgaactttca acaggatttt
     5161 gccaaattct tccggcgttt gcagacgcgt ctgcacaatg atcgaggcgt ttagttgctg
     5221 gtctgccgcc tgtggcatgc cacccagttg accaccggaa atctggttgt tttgcacctt
     5281 aatctgggaa ataacatcgg aaggtaccag attgtattta ttgagttttt gcgggtccag
     5341 ccagatacgc atggcgtact cggaaccaaa gagctgtacg ctaccaacgc ccgcggtacg
     5401 gcttagcggg tctttgatat tagacgctac atagtccgcg atatcgtact ggttgaggct
     5461 gccgttatca gaaataaacg ccgctaccat cagaatatta ctgctcgact tatcaacgct
     5521 aatcccctgc tgctgcaccg cttcaggtaa tgaaggcata gcgagttgca gtttattttg
     5581 cacctgaacc tgtgcgatat caggagatgt cccagtctcg aaggtcagag tgatagaggc
     5641 attacccgcc gcatcactgg ttgaagacat gtacatcagg ccatcaagcc cattcatatt
     5701 ttgctcaatc acctgagtga ccgagtcttc taccgtttgc gcatcggcac ctggataggt
     5761 agcgctgacg gtaatggtcg gtggcgcaat ctgcggatac tgcgcaaccg gtaagttcat
     5821 gatcgccaga ccacctgcaa gcatcataat aatggcaagt acccaggcaa aaaccgggcg
     5881 atcaataaaa tagttagcca tgaaagtccc cttaagcctg caacgttatt gtttcgattc
     5941 ggtgctggcg ttttcctggc tggaggaaat tgctcgtgct ttgatacccg gacgaatgcg
     6001 ttgcaaaccg gaaacgatca cccgatcgcc agcctgcaag ccagaggtga caacccactg
     6061 gtcgccgatg gctttgctgg cttcaatttc gcgtagctgc acaacatcgt ctttatccag
     6121 aatgagcgcc gttgctttac cctgggcgtt gtgggtgacg ccttcctgcg gcaccagtaa
     6181 tacattctgg cggctacctt catccactaa tgccgtgacg tacatgccag gcagcaagtc
     6241 accatttggg ttggggaaaa tcgcccgtaa cgtcacggag cccgtggttt catccaccgt
     6301 cgggtcggag aatttcagtg tgccggtctg gctgtagcgt ttaccatttt ccagattgag
     6361 ctgtactggc gtactgccct gaacctgttt gatttgccca ctggcgacct cttctttcat
     6421 gcgcaagaaa tcctgcaccg actgcgtgag atcgacataa atcgggtcca gacgttgtac
     6481 ggtaaccagc gaatctgcct gattagcggt aacgagtgcg ccgacggtca ccgacgattt
     6541 cccgctgacg cccgtaatcg gcgaggtgac attcgcgtat tgcagattga tcgtcgcctg
     6601 ttcaacagcc gctttggcga cggtgacatt ggcttctgct tcattcagct gggtgcgcgc
     6661 ggtgtcgtaa tcctgacggc taacgtagtt ggtcttcagc aacgatgcct ggcggttaaa
     6721 ggtgatgcgg gcattgctgg cggtagagag cgctttcgcc agcgagcctt tggcggagtt
     6781 tagctcggcc tgtaaaggtg caggatcaat ctgatacagc gaatcgccct ggttcacttt
     6841 atcgccttcg ataaagttgc gtttaatgat aataccgccc acctggggac gtatctcggc
     6901 aacttcataa ggaacggttc taccgggcaa ttcgctcaac acattgaccg aaccggggga
     6961 gagtgtgacg acaccgacct caggcgtcat ggcggcggcg ttttccgccg atttgtcatc
     7021 gcaggcggtg agcatcgcgc cgcagaataa caacggtatt aacagctttc ttcttctgtt
     7081 cattttagtc cctgaaaatt cttgagaaat aacggttctt taaattgcgc gcgaggagag
     7141 atatgaggga tttacagacc agcggcatag tggggttgtc agccagtaag gtgggataca
     7201 ggcacagtga tcgacatggt gaggtcaacg acaaaaaaaa tgttgccgtt ctgccaacag
     7261 ttcctgccag cattcgggca acgaagggtt ctactggtgg atacacatac caggggaata
     7321 aagtcgtttt agcctctcct ttagtaatca ccgggggtaa tgaaatcagc atctgcatcc
     7381 ctcgtcatgc cagccatcaa tttcagttgc ttatgtcctg actaaaaata agatgtgata
     7441 cccagggtga cgatgtcgct catacgtctt agtttcaact tgtacataat gttgaatttc
     7501 tgaatcttta atgtttgttg cgaaatattg agcgtcaacg tgatattgct ttcattttta
     7561 tttttcagaa tcagttgcat aattttgaac tgattatgtg aaaaacataa ttgtgaattc
     7621 ttatggggca agtgtttacc ataagtaatc tcaagaagat ttttgatact ttctttgcgg
     7681 ctaacaaaat aaataggcgc tttagctttt agtttactga tgtgataagg tgccaaaaca
     7741 ataattctct cggcatctaa tttctccaga aatttaatcg cttcttcatc aaggatatga
     7801 ttgtgatagg tatcaatgat aaccttgaag ggtttattgc ctactttttt gatctctgac
     7861 agggaattga tttttatcat ttcgtgatta tctttcaact gccaaaagcc ctgtaaaaga
     7921 aaagaatctt tcgtcatgag aaaaatcata acttgctcct tagccgttat cgtttcgaac
     7981 atgtcatcct tttcattatt tacatccttg tccgaatcgt tgttcaatat agtaaacgcc
     8041 tataagaact ttacccttcg tgaactaata cgtctattaa cactaatcaa tacttcagat
     8101 ttatccatca cgcactccat atattgatcc taagtaagat tcctggttgt tatcagcttg
     8161 taaatagtaa acaaaaagtt aacgtttgcg gattactgcc caagaataag ttatcaataa
     8221 ccagtttgtg atctctgaag aatattacta aagttaaaat gtaatccgat ttaaatatcg
     8281 agtctccttg tttcgactta agctggcaat tggattgcca gctttctttt aacgcattga
     8341 aattacctga gtaaataacg cattcatgtc ttcaggtttt tatatagcgt ctggagacac
     8401 ggatatttat gcaatgtaat caacgccagt tgcaaaaaaa tgaacccgga ttacttattt
     8461 aaccgcccat agcatcaacg cttacggaac tctttgttca ctttcccaca gcacttttaa
     8521 aacattaaac ctgactacgg aaaatatcag ccatgataat gtttgaaatg gctaatttgc
     8581 catggagtga aaaaaattag atgaaattca gtaggttgaa ataatcacta gcaggtaatt
     8641 atttcaatga tagtgcgcaa ttgatctaca acactgcgta gcggagagag tattaatcgg
     8701 atcatagtca catcaagtga ctatgatccg ggtgacaacc ggggtaatta ttgctgctta
     8761 acgaacaaac tggcgaagct gaacaggctg gcggcgctaa atatcagttc aattcccacc
     8821 agtgtggaaa ccagcgttac agacaccatc ggcgttgcac caaggaatat ccaggcaatg
     8881 acgatatcca gcacaccaat aacgagctgt agccagctgc ctttcattga acgctgacga
     8941 taccaactca tcaggcgaat aacccctgca acacagaaca aaccggcaat aaatgccgca
     9001 atggcaaaaa tgcccagctc cggtgcgcgg atgaagaaat agccgatcaa taaataggcg
     9061 actgcgacga ggaaaccgga taataccggc cagaaattat gactgcggtt gctgaataac
     9121 ccgacaataa gcgcaatacc cgagcagatt aataatgcac ccactactgt gcttaaaata
     9181 tcgccagaga cgaacgggaa actgatacac agcaacccga cgataaacag cagcacggca
     9241 ataaactgga ttgctctgcg atgtttttta agcatctcca gatcaaactt caaaattgtt
     9301 gccttatcta tatataacat agaaccaccc tataaaatta agaagaaaat cccctgctat
     9361 caatctatgc caaaaacgcg tctaagaatg cagtcgattt aataaaaatt tcctaattgc
     9421 agtatctgat gcatctgtaa ctcattgtat tgaaataaaa atatctgatt ttgatatttt
     9481 ccatcaacat gacatataca gaaaaccagg ttataacctc agtgtcgaaa ttgatttgtg
     9541 acggctcttt cactttatag ttgaggatat tacgatgaaa aaagtattag gcgttattct
     9601 tggtggtctg cttcttctgc cagttgtgag caatgcagcg gatgcgcaaa aagcagctga
     9661 taacaaaaaa ccggtcaact cctggacctg tgaagatttc ctggctgtgg acgaatcctt
     9721 ccagccaact gcagttggtt ttgctgaagc gctgaacaac aaagataaac cagaagatgc
     9781 ggttttagat gttcagggta ttgcaaccgt aaccccagct atcgttcagg cttgtactca
     9841 ggataaacaa gccaacttta aagataaagt taaaggcgaa tgggacaaaa ttaagaaaga
     9901 tatgtaattc cgggaatgcg ttacatcgta cttccttgca tattgaacag gccggaatat
     9961 cttctttaaa agcagctatt cctcctgttc atatataatc tatatattga atgggttaca
    10021 aaatgaatat ttcatctctc cgtaaagcgt ttatttttat gggcgctgta gcggctttgt
    10081 cactggtgaa cgcacaatct gcgctggcag ccaatgaatc cgctaaagat atgacctgcc
    10141 aggaatttat tgatctgaat ccaaaagcaa tgaccccggt tgcatggtgg atgctgcatg
    10201 aagaaacagt atataaaggt ggcgataccg ttactttaaa tgaaaccgat ctcactcaaa
    10261 ttcctaaagt gatcgaatac tgtaagaaaa acccgcagaa aaatttgtat accttcaaaa
    10321 atcaagcatc taatgacttg ccgaattaat gaggtacaag taaaaaggag tagcaagttg
    10381 agccatcttg ctgctccttt ttgcattttt atatgacagc agaatttatt atacgtctta
    10441 tactcgcggc aattgcctgt ggcgctattg gcatggaaag gcaaatgtga ggcaaaggag
    10501 cagggttacg cacacatgta ttaattggca tgggaagcgc cctgtttatg attgtttcga
    10561 aatatggttt tgctgacgtg ctgtctttag atcacgtcgg actcgacccc agccgtattg
    10621 ctgctcaggt ggtgacgggc gtcgggttta tcggtgcagg taacattctc gttcgtaacc
    10681 aaaatattgt aggtttgacg acggcagcgg atatctggat gaccgccgct ataggtatgg
    10741 ttattggcag tggtatgtac gaattgggta tgtatggttc agtgatgacc ttgctggtgc
    10801 tggaagtctt ccatcaatta accttccgcc tgatgaacaa aaattaccat ttacagctga
    10861 cattagtgaa cggtaatacg gtatcaatgc tcgactggtt taagcagcaa aaaataaaaa
    10921 cggatttagt ttcgttgcag gaaaatgaag atcatgaagt ggtcgccatt gatatccagc
    10981 ttcatgccac aacgtcgata gaagatctgt tgcgattatt aaaaggtatg gcaggggtga
    11041 aaggcgtttc aattagctaa tctgtgtggc agcgtagtca gactcaccgt aagcctgaaa
    11101 ttcacaccag ataatcaata tgctgatggc gtaccagctc attgatccgc ttaagattga
    11161 ggatcatcat gatattgtgt ttatggcagt agaacgtttt tagcgttatt ttcagtttct
    11221 ccagaatatc ctccgttgtc atttgattcg caatgcagct cagaacctga tgttgcttgc
    11281 gcgaaagtga cacagccgct tcaggcacag agcagtaact gccattgatg agtgcgacaa
    11341 aatcgatgat agtgatagtc cgcggaacaa aggtcaccgg cactttcccc aggcagcgtt
    11401 taatattaaa gggggagaaa ataatcacat ggacaggttt taatcgttca atttgagtga
    11461 gaaaattaga atgaaataca ttattcatta atgtatcaat aatgacaaag gcattctgat
    11521 gcaacattcc gtcgatctct tcttcgttct gtatatgaac gacattacct ttgctcaaaa
    11581 tgtttttcat cgcggtgaag aacatcgtat ccctggtaat tataagaaac ataaccgact
    11641 cctggtgcgg acattaattt tataatcagt ttgccaaagg taattaacag gacagggctt
    11701 ataataataa tagttagctt gctgtgtatt aattttatgt aatgcacgat gcagatgctg
    11761 agaaagcatc tgcatctttc ggtggtgtta tttgaccagc tcaggtgtta cctgactcac
    11821 cgcattggtg tagtaaggcg caccccagcc cggttccggc cagaatgcac catagccgta
    11881 atcccatagg ttataggtgg tattaaccac ttctctcaaa tgccacacct ggatgccctg
    11941 catattcact tccaggaagt tatacgggac tttattgata aagccaggtt gttcaccctg
    12001 aatggtaccg aggatggtaa caaagtgatt acgatagttc actgggtcaa ggaagccgct
    12061 ttgtctggcg agcagtcggc cctgatagtt ggcttcaata tcaggtttcg catagctatc
    12121 caacggtaat acagcgattt ctaacaacgt atccgttttg ccattgataa cgttgataac
    12181 cttcccacca aagcgcgctt gttgaccaac atataacccc ggctggttat gaacagcaac
    12241 aaaacttttt tgaatatcag gttggttatt gcctttgata ttttgcggaa ttgaactaca
    12301 tgcggcaagc aaaaatgaaa ggctgaggat gagtgcacct tttgtcatgt tcatgttact
    12361 atccttatca acaaatatta aaaccataat agatatttct ttatagtttt catctgattc
    12421 tgaggcgata tgcctgatag taacgtaaac ctgtctgatg tttatatccc ttttgtttag
    12481 ttcaattcca ttgattttgg ggcgtggcat aagtatccat cgccccataa tataatcctt
    12541 tgttttaaaa ttacataaaa tctattttta ttcaataatt ataaagtgct aataatcaat
    12601 attatggtat tatattctga taatgatttg ataagcgaat tcttttgcca gatggtattt
    12661 ctatttccac cccataattt atatcttttt cattaggaag tggcctcttt aaataaggcg
    12721 tgtgtgacat tttttttggg ttaagcgtct cgaattgaaa tacttcgaac tgatattcag
    12781 acatttctgc ccatgtttga attttattgt atcgagagag tgtcgtagag gctttaactt
    12841 gcttataaca ttgtttttgg aattcttgct gatattttaa tatgtcattg tatgaaagat
    12901 tggttatcct ggcctctaaa aatttaatta ttgtcgaagc aatttccgct tttgctcgta
    12961 aaaagttgtc attaatggtt aataatgttg aaaataaacc ttcgatttgt ttgtcaagat
    13021 attgtccatc tttatataat ttggggggta gggtgttctt tatgtaaaaa atagtgtcac
    13081 ctaaatttgc gtcagtgaaa tttatattaa gtagatcttc gaaataaaac cttgacaagg
    13141 ttagaacgaa cccggaaagg ttctgtcctg tcatattgaa atcaataaag ttggttccgg
    13201 caaagacttt catcctgttt ttattttcac tgtgctcaat atataacttc gtcatttttt
    13261 gatacaggct ttgaatatct tttatattag ttggtaagtc agctcgttgt tcagcaataa
    13321 aattagtatc tgaaaacatg gtttggacac tgaatgttat gggtattccc tcttctacgc
    13381 taattttagt tatgattgcc ttgtcgggtt taggattagc gacaacaatg attcgacatt
    13441 cagcactttt gtttttttca tatctaatgt tattaatctg attaagtatt gattgtgtct
    13501 gttgcgtaag tggtgtcgaa cttatcgttt caatgttatt aataagactt acaagttgaa
    13561 ccacaacttt tgagtagtct gcatttgtat atgtggtgtt cgtgtggcat gcattaattt
    13621 gttggagttg ttgttgcgct aaatcaaatt gttcagggtt gtatccttct ccagagaatg
    13681 tggaattaat gggttgttgt tcttcccaat tatcacaagg ccacttcggt gtgtctggcg
    13741 ttttaacatt atcggtattt gttgtgcagt tataaatatt gccgtgatat gtatttatta
    13801 tacctggggt aaagtcaatt gacataacat tccttattca tcatctgttt tatgctaagg
    13861 tttttaaatt ggataattag aacaggaatg gattttcttc tacacacaat aaatataatg
    13921 cgttatatct tcctgatata attaccttta aattcccttt catttccacc cggtaatatt
    13981 tgtgtataaa aaaagcagtg atagtatcgt aaaggaaaac gttaacaact atcaaaaagc
    14041 gctcatttta aaaagttatt atgttctccg atagtgtgtg aagtaatatt tttgtgatgt
    14101 tttagatgtg taataatgaa ccaggttaaa gtgtatttaa aaatgtgttg tattagcgac
    14161 tgaaatctgc cttttaaaaa ggaggagggt actgagacaa agagtcattg cttctccgat
    14221 a
//
LOCUS       DADLFA010000102        14198 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000102 DADLFA010000000
VERSION     DADLFA010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14198
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.102"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(92..388)
                     /locus_tag="JQH65_002576"
     CDS             complement(92..388)
                     /locus_tag="JQH65_002576"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6139954.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSIFSDYSFSIEMHNNLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
     gene            672..2303
                     /gene="oppA"
                     /locus_tag="JQH65_002577"
     CDS             672..2303
                     /gene="oppA"
                     /locus_tag="JQH65_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HAY6139955.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2389..3309
                     /gene="oppB"
                     /locus_tag="JQH65_002578"
     CDS             2389..3309
                     /gene="oppB"
                     /locus_tag="JQH65_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HAY6139956.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3324..4232
                     /gene="oppC"
                     /locus_tag="JQH65_002579"
     CDS             3324..4232
                     /gene="oppC"
                     /locus_tag="JQH65_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HAY6139957.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFIHNRAAVACLM
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            4244..5257
                     /gene="oppD"
                     /locus_tag="JQH65_002580"
     CDS             4244..5257
                     /gene="oppD"
                     /locus_tag="JQH65_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HAY6139958.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTLGRLCACFKPVE
                     ELL"
     gene            5254..6258
                     /gene="oppF"
                     /locus_tag="JQH65_002581"
     CDS             5254..6258
                     /gene="oppF"
                     /locus_tag="JQH65_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HAY6139959.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGIKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6311..6640)
                     /locus_tag="JQH65_002582"
     CDS             complement(6311..6640)
                     /locus_tag="JQH65_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HAY6139960.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPVEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6675..8135)
                     /gene="cls"
                     /locus_tag="JQH65_002583"
     CDS             complement(6675..8135)
                     /gene="cls"
                     /locus_tag="JQH65_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAY6139961.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEIVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            8278..8451
                     /locus_tag="JQH65_002584"
     CDS             8278..8451
                     /locus_tag="JQH65_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HAY6139962.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8506..9759)
                     /gene="kch"
                     /locus_tag="JQH65_002585"
     CDS             complement(8506..9759)
                     /gene="kch"
                     /locus_tag="JQH65_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HAY6139963.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCLAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(10059..10355)
                     /locus_tag="JQH65_002586"
     CDS             complement(10059..10355)
                     /locus_tag="JQH65_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009448315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HAY6139964.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            10570..11298
                     /gene="tonB"
                     /locus_tag="JQH65_002587"
     CDS             10570..11298
                     /gene="tonB"
                     /locus_tag="JQH65_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HAY6139965.1"
                     /translation="MTSMTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTALARLTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            complement(11338..11736)
                     /gene="yciA"
                     /locus_tag="JQH65_002588"
     CDS             complement(11338..11736)
                     /gene="yciA"
                     /locus_tag="JQH65_002588"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HAY6139966.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(11841..12380)
                     /locus_tag="JQH65_002589"
     CDS             complement(11841..12380)
                     /locus_tag="JQH65_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HAY6139967.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(12410..13153)
                     /locus_tag="JQH65_002590"
     CDS             complement(12410..13153)
                     /locus_tag="JQH65_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HAY6139968.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAVLLIYLFRLYMLIRQ"
     gene            complement(13181..13270)
                     /locus_tag="JQH65_002591"
                     /pseudo
     CDS             complement(13181..13270)
                     /locus_tag="JQH65_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            13510..14148
                     /gene="ompW"
                     /locus_tag="JQH65_002592"
     CDS             13510..14148
                     /gene="ompW"
                     /locus_tag="JQH65_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HAY6139969.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
BASE COUNT         3825 a         3319 c         3475 g         3579 t
ORIGIN      
        1 cgcagttatc aaaagcaaaa ggaataggta aaaatattct tctcaaatta cagttagtta
       61 taaggatttt cttaactgct tctcctcacc atcatgttat tttcgccaca tcataatcct
      121 gggcttgctg aagaataatt gaaatgatat tagtaattcc actgcctttg gtagaggaaa
      181 gtgctaaata ataatcaatt gttaaattat tgtgcatttc aatactgaaa ctgtaatcag
      241 aaaagataga catgcttagc caatctctat ttgattgaat tgaaagatgt ttgttaaggc
      301 atggatgcaa gctatagatt ctgatacggt caataaaaga gaattgctta acaattttgc
      361 aaaatgtatt ggcgagtaag aaccgcattt agtactttcc gggcaaccgc cagacgattc
      421 tttattggta atgagaataa ttaacaatta aagagcgtcg cgaaagaata atgtgtctcg
      481 acaggggaga cacagtacga atcgacataa ggtgatcgtc tgaatcacca gaataaataa
      541 agtcggtgat agcaatccgt aacgataaag taacctgaca gcagaaagtc tccgagcctg
      601 tgcagggtcc caatccggga ttacacatgc tggttaatac cagtaattat aatgagggag
      661 tccaaaaaac aatgaccaac atcaccaaga gaagtttagt agcagctggc gttctggctg
      721 cgctaatggc agggaatgtc gcgctggcag ctgatgtacc cgcaggcgtc acactggcgg
      781 aaaaacaaac gctggtacgt aacaatggtt cagaagttca gtcattagat ccgcacaaaa
      841 ttgaaggtgt tccggagtct aatatcagcc gagacctgtt tgaaggctta ctggtcagcg
      901 atcttgacgg tcatccggca cctggcgtcg ctgaatcctg ggataataaa gacgcgaaag
      961 tctggacctt ccatttgcgt aaagatgcga aatggtctga tggtacgcca gtcacagcac
     1021 aagactttgt gtatagctgg caacgttctg ttgatccgaa cactgcttct ccgtatgcca
     1081 gttatctgca atatgggcat atcgccggta ttgatgaaat tcttgaaggg aaaaaaccga
     1141 ttaccgatct cggtgtgaaa gctattgatg atcacacatt agaagtcacc ttaagtgaac
     1201 ccgttccgta cttctataaa ttacttgttc acccatcaac ttcaccggtg ccaaaagccg
     1261 ctatcgagaa attcggcgaa aaatggaccc agcctggtaa tatcgtcacc aacggtgcct
     1321 ataccttaaa agattgggtc gtaaacgaac gaatcgttct tgaacgcagc ccgacctact
     1381 ggaacaacgc gaaaaccgtt attaaccagg taacctattt gcctattgct tctgaagtta
     1441 ccgatgtcaa ccgctaccgt agtggtgaaa tcgacatgac ttataacaac atgccgatcg
     1501 aattgttcca gaagctgaaa aaagagatcc cggacgaagt tcacgttgat ccatacctgt
     1561 gcacttacta ttacgaaatt aacaaccaga aaccgccatt caacgatgtg cgtgtgcgta
     1621 ccgcactgaa actaggtatg gaccgcgata tcattgttaa taaagtgaaa gcgcagggcg
     1681 acatgcccgc ctatggttac actccaccgt atactgatgg cgcaaaattg actcagccgg
     1741 aatggtttgg ctggagccag gaaaaacgta acgaagaagc gaaaaaactg ctggctgaag
     1801 cgggttatac cgcagacaaa ccgttgacaa tcaacctgtt gtataacacc tccgatctgc
     1861 ataaaaagct ggcgattgct gcctcttcat tgtggaagaa aaacattggt gtaaacgtca
     1921 aactggttaa ccaggagtgg aaaacgttcc tcgacacccg tcaccagggt acttttgatg
     1981 tggcccgtgc aggctggtgt gctgactaca acgaaccaac ttccttcctg aacaccatgc
     2041 tttcgaacag ctcgatgaat accgcgcatt ataagagccc ggcctttgac agcattatgg
     2101 cggaaacgct gaaagtgact gacgaggcgc agcgcacagc tctgtacact aaagcagaac
     2161 aacagctgga taaggattcg gccattgttc ctgtttatta ctacgtgaat gcgcgtctgg
     2221 tgaaaccgtg ggttggtggc tataccggca aagatccgct ggataatacc tatacccgga
     2281 atatgtacat tgtgaagcac taatggcaat acgtggggca ggagtgtcct gctccacggt
     2341 gtctgatttt tatcgcatta cagaaggcac aggccagaag gtagggcaat gttaaaattt
     2401 attctacgtc gctgtctgga agcgattccg acgctattta ttcttattac tatttcgttc
     2461 tttatgatgc gcctcgcgcc gggaagccct tttaccggcg aacgtacttt accgccagaa
     2521 gtgatggcca atatcgaagc gaaatatcat cttaatgatc cgatcatgac acagtatttc
     2581 agctacctga aacaactggc gcacggtgat ttcggtccat cgtttaaata taaagattat
     2641 tcggtcaatg acttggttgc atccagtttt cccgtttctg ccaaactggg agccgcagca
     2701 tttttccttg cggtaatact gggtgttagt gctggcgtta ttgccgcatt aaaacaaaac
     2761 accaaatggg actataccgt gatggggctg gcaatgaccg gggttgttat ccccagtttt
     2821 gtggttgcac cattattagt catgatattt gcgatcattt tgcattggct gccgggcggt
     2881 ggctggaatg gtggggcgct taaattcatg atattgccga tggtggcgtt gtcactcgct
     2941 tatatcgcca gtattgcgcg tattacccgt ggctctatga ttgaagtatt acactccaat
     3001 tttattcgta ctgcccgggc gaaagggtta cctatgcggc ggatcatttt acgtcacgca
     3061 ttaaaacctg ctctgttacc cgtgctctct tatatgggcc ccgcatttgt cggcattatt
     3121 accggttcta tggttatcga aaccatttat ggtttgccgg ggattggaca actgttcgtt
     3181 aatggcgcat tgaaccgtga ctattcctta gtgttaagcc tgaccatcct ggttggtgct
     3241 ttaaccattt tgtttaatgc cattgtcgat gtgctatatg cggttatcga cccgaaaatc
     3301 cgttactgat actggagctc gcgatgatgt taagtaagaa aaacagcgag acgctggaaa
     3361 atttcagtga aaagctggag gtcgaagggc gcagcttgtg gcaggacgca cgtcgacgtt
     3421 ttattcataa ccgtgcggcg gttgcctgtc tgatggtgct ggtgctgatc gcgttatttg
     3481 taatcctggc accgatgctt tcccagtttg cctatgacga tactgactgg gcgatgatgt
     3541 ccagcgcccc ggatatggag tccggtcact actttggtac tgactcatcc ggtcgcgacc
     3601 tgcttgtgcg cgttgcgatt ggtgggcgta tctcactcat ggtcggtgtt gctgcggcac
     3661 tggtggctgt ggtcgtcggg acactttatg gttcgctttc cggttatctg ggcggtaaag
     3721 tggattcggt aatgatgcgt ctgctggaaa tcctcaactc cttcccattc atgttcttcg
     3781 tcattttgct ggtgaccttt ttcggtcaaa acatcctgct gattttcgtg gcgataggca
     3841 tggtttcctg gctggatatg gctcgtattg tgcgtgggca aaccctgagt ctgaagcgca
     3901 aagagtttat tgaggcggca caagttggcg gtgtatcgac atcgggcatt gttattcgcc
     3961 acattgtgcc gaatgtactc ggtgtggtgg tggtctacgc atcacttctg gtgcccagca
     4021 tgatcctctt tgaatctttc cttagcttcc tggggttggg tacgcaagag ccgttaagca
     4081 gctggggtgc attgctgagt gatggcgcga actcgatgga agtctctcca tggttattgt
     4141 tgttcccagc gggattcctc gtggtgacgc tgttttgttt caactttatc ggcgatggct
     4201 tgcgtgatgc cctcgacccg aaagatcgtt aaggagtgca gccatgagcg taattgaaac
     4261 tgcaactgtg ccgctcgcac aacaacaggc tgacgcactg ctgaacgtga aagatttgcg
     4321 tgtcaccttt agtaccccgg acggcgacgt cacggcggtc aatgatttga atttttccct
     4381 acgtgccgga gaaacgctgg gtattgtagg tgagtctggt tcgggtaaat cgcaaactgc
     4441 atttgcgttg atgggcctgt tggctgccaa cggacgtatt ggcggatcgg caaccttcaa
     4501 tgggcgtgaa atcctcaatt tgccagagca tgaactcaat aaactgcgcg ctgaacaaat
     4561 ctcaatgatt tttcaggacc caatgacttc gttgaatccc tatatgcgcg tcggtgagca
     4621 gttgatggaa gtgctgatgc tgcataagaa catgagcaaa gctgaggcgt ttgaagagtc
     4681 ggtgcggatg ctcgatgcgg taaaaatgcc ggaagcacgt aaacgcatga aaatgtaccc
     4741 gcacgagttt tctggcggca tgcgtcagcg agttatgatt gcgatggcat tgctatgtcg
     4801 acctaagctg ctgattgcgg atgaacccac tacagcgctg gacgtcaccg tacaggcgca
     4861 gatcatgacg ctattaaatg aactgaaacg ggaatttaat accgccatca ttatgattac
     4921 ccacgatctt ggcgtggtgg cggggatctg tgacaaagtg ctggtaatgt acgccgggcg
     4981 cacgatggaa tatggcaacg cgcgcgatgt cttttatcaa cccgttcatc cttattctat
     5041 cggtttgctc aacgcggtgc cgcgtctcga tgcggaaggt gaaacaatgt tgaccatccc
     5101 gggtaatccg ccaaacctgc tgcgattacc gaaaggttgc ccgttccagc cacgttgtcc
     5161 gcatgcgatg gaaatttgta gtagcgcacc gccgctggaa gagtttacgc ttggccgtct
     5221 gtgtgcttgc tttaaaccgg tggaggaact gttatgaatg ctgtaaccga aggaagaaaa
     5281 gtcctccttg aaatcgccga tctgaaagtg cactttgaaa tcaaagatgg caaacagtgg
     5341 ttctggcaac cgccgaaaac gctcaaagcc gtcgatggtg tcacgcttcg cctgtatgaa
     5401 ggggaaacat taggtgtggt aggggaatcg ggatgcggta agtccacctt tgctcgcgcc
     5461 atcattggcc tggttaaagc gaccgacggt catgttgcct ggttaggtaa agagttgctg
     5521 ggcattaagc ccgatgaatg gcgtgccgtt cgcagtgata ttcagatgat tttccaggat
     5581 ccgttggcat cgctaaaccc gcgcatgacc atcggcgaga tcatcgctga accactgcgt
     5641 acttatcatc cgaaaatgtc acgtcaggaa gttcgcgagc gcgtgaaggc gatgatgctg
     5701 aaagtcgggt tattgcctaa cctgattaac cgctatccgc atgagttctc cggtgggcag
     5761 tgccagcgta tcgggattgc tcgtgctctt attcttgaac cgaagctgat tatctgtgat
     5821 gagccggtgt cggcgctgga cgtgtcaatt caggcgcagg tggtcaacct gctccagcag
     5881 ctgcaacgtg agatgggatt gtcattaatt tttattgctc atgacctggc cgtggtaaaa
     5941 cacatttccg atcgtgtgtt ggtgatgtat ctcggccatg cggtagaact ggggacctat
     6001 gatgaggtct accacaatcc actacatcct tacaccaggg cattgatgtc ggcagtcccc
     6061 atacctgatc cggatctgga gaagaacaaa accatccagt tactggaagg ggaattaccg
     6121 tcgccgatca acccgccttc cggttgtgtt ttccgtaccc gttgcccgat tgccggtccg
     6181 gagtgcgcca aaacacgtcc tgtgctggaa gggagtttca gacacgccgt ttcttgcctg
     6241 aaagtcgatc cgctttaaac acaataaggg ctgacagttg tcagcccttt ttcacgccga
     6301 aagcgattat ttattcccgc cagatgatat ggcaaagttt gtgatctttt tcgcggcata
     6361 acagaatgcg ggcgaaaaca tcgttgattt caccatcctc actgtccgcc agaccaatca
     6421 ccacttcggc aaaaaagtcc gggttcaaat cgaaatcaac atgttcctgc cagtcttcca
     6481 ccggatcgaa taactccgcg ccgccgcgct cttcaaactg aagattgaac agcagaacat
     6541 cagctgggtc gagattgtcg gcagccagtt cgagaaaaat atcgtaggcc tgctcaagcg
     6601 tttcatcttc agtcaggcga ttgtttagat ccatatccat agtcactacc tgtttaacct
     6661 ctgttggaga cgttttacag caacgggctg aagaagtaaa acagtcgctc ggcgacacgt
     6721 tgccataatg gacgttttag ccataaacgg gcatcgagca gacgtgaacg cgaaatataa
     6781 tcgtcctgaa cggcggcaag gtctgcacca aaacctttat cgtcgattgc cagggtaatc
     6841 tcgaaattca gccacagact acgcatatca aggttaacgg tgccaaccag acttagttcg
     6901 ccatcgacca gcacgctctt ggtatgcagt aacccgcctt caaactgata aattttaacc
     6961 ccagcagcaa gaagttccgt aaagaatgcg cgactggccc agccgaccag catcgagtca
     7021 ttttttcgcg gaaggataat actgacatcc accccgcgct gtgccgccgt gcaaatcgca
     7081 tgaagtaaat catcgcttgg cacaaagtag ggcgtggtca tgatcaaata ttcacgcgcc
     7141 gaataagccg cagtcaataa tgcctggtga atgagatctt ccggaaagcc ggggccagaa
     7201 gcaattgtgt gaatggtgtg accgctggcc tgttcaaacg gcataatatt gacatctggt
     7261 ggtggcggca gaatacgttt tccggtttca atctcccagt cgcaggaata aataatcccc
     7321 atcgcggtgg cgatggggcc ttccatacgc gccatcagat caatccattg ccctacgccc
     7381 gcatcttgtt tgaagtagcg aggatcgacc atattcatgc tgccggtgta cgcgatgtaa
     7441 ttatcgatca tgatcatctt gcgatgttgg cgcaggtcca tacggcgtaa aaacacacgc
     7501 atcagattga cctttaaggc ttcgaccact tcaataccgg cattacgcat tagctcgggc
     7561 cacgggctgc ggaaaaaagc cacactcccg gcggagtcga gcatcaatcg gcaatgaatg
     7621 ccgcgtcgcg cagcggccat taatgattca gccacctggt ccgccatgcc gccgggctgc
     7681 cagatataaa acacaatctc aatattatgg cgcgcgagct ggatgtcgcg gattaacgcc
     7741 tgcatcacat catctgactc ggtcatcagt tgtagctgat tccctttgac cccagcgatc
     7801 ccctgacgac gctcgcaaag cttgaataat ggcgcagcga cactgctatt ttcttcggcg
     7861 aagatatgct tacaggcttt aaggtcgtta agccattttg cggtggaagg ccacatcgct
     7921 ctggcgcgct cagcgcggcg tttgcctaaa tggagctcgc caacggcaag ataggcaata
     7981 attccgacta acggcagaat gtaaataatc aacagccagg ccatcgcgga gggaactgcg
     8041 cgtcgtttca ttagaatgcg taaagttacg cctgcaatga gcaaccagta tcccagaatg
     8101 gccaaccaac tcaccaacgt ataaacggtt gtcatagatt agaaatcctt ttgaaagcgc
     8161 attgttatga gtttacgcat atggattcat ctggcaaata aaaacgcggg aaaagcactg
     8221 gtcagcgtcg ggggcggggg tataatgacc attctgttat tgcatagagt agttaacatg
     8281 aagcggagta gaacggaagt ggggcgctgg cgcatgcagc gtcaggctag ccgacgtaaa
     8341 tcgcgttggc ttgaggggca atcgcgccga aatatgcgta tccacagcat caggaagtgc
     8401 attctaaaca aacagcgtaa ctcgttattg tttgcgatct acaatatcta aatgtactgg
     8461 gcaccattgc ggtgcccaat aagtttactc ctccatcttt aaactctatt tttgcgccga
     8521 ttctttactg tcagctttcg tttcttgttc atcgttatcg ctgaaaatgc catgaccgga
     8581 gttcaacaac attgaaacga gcatatcatt attaatctct tcaccattta aaactcgcgc
     8641 cagaatttcg ctgccaaaca gttgcggtga gagaatgata tccggatgta ccatcttaat
     8701 cttattcagg tttttgctat cactgacggc gagaactgtt ttgacatcac tgctcatatc
     8761 ttttgccgag agtacaacaa acgcgttatc tgcatcgtta tcactcagcg ccagaatggc
     8821 caggcaacga tcgattcccg ctttctttaa tactgaactg tcattactgt caccggggat
     8881 aacatcagcg ttatcgccta agcgttgctc aagttgcttg atatcatctt caggcaagtt
     8941 gctgataacc gttacgtttt gtccgcgttg attcagttgc agaatcgtat tgatggcgag
     9001 aatcgaatgt ccgcaaacaa taaaatgatc tttacgatgc attgtatgat tgtttccttt
     9061 tacaagtttg ttgaatcccc cgcggataag cggaccaaaa attgaggtca tagatgtggc
     9121 aaaaacggta ataccggaaa taatgaccga aatagtgaac aatcgtgctg attcagaaac
     9181 agggacaata tcgccatagc cgacggttga catggtttct atcgaaaaat agaacgcggt
     9241 catcaaactt tctattcgcg gattaaaacc ttcgcttaaa taaagcgcac cgtaggttga
     9301 gtaaaacagt aatgtcgtga aactaataaa tgcaaaaatt gtcccggctg cggcgctact
     9361 gtgggagaag tctttgcgca gtatcagcaa aaacaccagc gtaaaaatgc aaaatccaat
     9421 actaaatttc aaccagggat aaaaatgcag ggtataaatt agcgctatca acaacaaaat
     9481 gatactgatt gcccatgcga gctttgcccg gaacagtaag ccaatggagt tcaggacaag
     9541 aaagacgccg agcataaata gtggggcgtt tgccagcaag gagagatcca actctgaaaa
     9601 tgctttgata tggaaaatat ctagcaaatt gaccgacata ccatagattg ttttaaaaat
     9661 aagcaatcca tttaaaaaga cggccagcgc gagaatgtcg tgccgtaggg tcacccataa
     9721 atttgttgct gtttgtttga atgtagccca gtgactcact gatacctctg taaccacaat
     9781 tttgtttaat tatcttagcg tgcacatttt ctgaacatac atgcagcgcg tgaatgtgtt
     9841 aaacaatagt agatgacttt atccgcagta atcaaaggtt ttgagcgagc aactgtacct
     9901 caggaaaggt catttttcct gaatatgctc acatcatata aagaaataca gataaagtta
     9961 ttatctgctt gtggtggtga atgcactgac cggctataag gaaaggccaa acaagaacac
    10021 ggttgcaaaa accgtgccct taaatattga atctctattc agaacacttt cttaaatggt
    10081 ttcactgaaa cgtgttcata gactcctgcc gctacgtacg ggtcagcatc ggcccaggcc
    10141 tgagctgctt ccagcgattc aaattcagca ataacggttg agccagtaaa tcccgcagcc
    10201 cctggatcgt tactgtctac cgctggcatt ggaccagctg tcaacaaacg accttcatca
    10261 tgcagtaact gtaaacgtgc taaatgtgcc ggacgaacgg aaaggcgttt ttcgaggcta
    10321 tcagctttgt cttgagcgta aataacatac aacacgggca caactccttg ttcgggaaag
    10381 ttgtaaagta cgttatttga aagggcgaag atctgcaacg gaaagatgat gtctttgtta
    10441 agaccatgca taaagtaagg ttaattacgg caaaaatgac attttcactg atcctgatcg
    10501 tcttgcctta ttgaatatga ttgctatttg catttaaaat cgaggcttgg tttttctact
    10561 gaaatgatta tgacttcaat gacccttgat ttacctcgcc gcttcccctg gccgacgtta
    10621 ctttcggtct gcattcatgg tgctgttgtg gcgggtctgc tctatacctc ggtacatcag
    10681 gttattgaac tacctgcgcc tgcgcagcca atttctgtca cgatggttgc gcctgctgat
    10741 ctcgaaccgc cacaagccgt tcagccgcca ccggagccgg tggtagagcc agaaccggaa
    10801 cctgagccga tccccgaacc gccaaaagaa gcaccggtgg tcattgaaaa gccgaagccg
    10861 aaacctaagc caaaaccgaa gccggtgaaa aaggtacagg agcagccaaa acgcgatgtc
    10921 aaacccgtag agtcgcgtcc ggcatcaccg tttgaaaata cggcactggc acgcctgaca
    10981 tcaagtacag caacggctgc aaccagcaag ccggttacca gtgtggcttc aggaccacgc
    11041 gcattaagcc gtaatcagcc gcagtatccg gcacgagcac aggcattgcg cattgaaggg
    11101 caggttaaag ttaaatttga cgtcacgccg gatggtcgcg tggataacgt acaaatcctc
    11161 tcagccaagc ctgcgaacat gtttgagcgt gaggtgaaaa atgcgatgcg cagatggcgt
    11221 tatgagccgg gtaagccagg cagtgggatt gtggtgaata tcctgtttaa aattaacggc
    11281 accaccgaaa ttcagtaagc agaaagtcaa aagcctccga ccggaggctt ttgactatta
    11341 ctcaacaggt aaggcgcgag gttttccttc aggatcaacc gcgacatact taaataatgc
    11401 ttctgtcgct ttatagcgtt gcccgattgg ttcagacgct acttttttca cccacacttc
    11461 aatattaatg ctgaccgatg tcgtcccttt ctggacacag cgtgcatagc agcacaccac
    11521 atcgccgacc gcaaccggcc gtaagaaagt cattccttca acccgcacgg tcactacgcg
    11581 accgtgggca atttctttcg ccagaatagc gccgccaata tccatttgtg acattaacca
    11641 accaccaaag atgtcaccat tggcattggt atcggcgggc atggctaaag tacgtaaaac
    11701 aagatcgccc tgagggacgt tatgtgttgt agacatggta aaaccgactt aaaaggaaaa
    11761 tcacaggcgc gatgctacta tgattttcct gaggagaaca gaggagatac agcgccagcc
    11821 ccgaaggact ggcagtctgg ttaggattta tcttcctgcg gcatgtggcg gtagatatag
    11881 ataccgctta acaatgtgaa gattaaggta agggcggtca gtccaaagac tttaaagttg
    11941 acccaaatat tttgcggcag ccagaatgcg atgtagatgt ttgccagacc gcaaaggata
    12001 aagaaaacag cccaggccag attcagcttc gaccataccg gttgcggcag cgtgagttct
    12061 ttacccagca tccgctgaat tagcggcttt ttcatcaccc attggctgac taacagggct
    12121 cccgcaaaca gagcataaat gactgtaacc ttccatttaa taaactcatc attgtggaag
    12181 aacagcgtca agccaccgaa gacgaccacc agaacaaaag tgatcagggc catcttctca
    12241 accttacgaa agcgaaccca gctatagata agcacaatcg ccgtggcgac gatcagcgcc
    12301 gcagtagccg catagatgtc ataaatcttg taaaacgcga aaaagacaac cagcggtaaa
    12361 aaatcaagaa actgcttcat tttacgattc cgtaatcaaa tgcttaaggt tattggcgaa
    12421 tcaacatata cagacggaac agatagatga gcaatacggc tgaaatcagg ttgctcaagg
    12481 tgttcgccag tacggcacca atttccgggg ttaatgcggc aaaagaagag gcaaaaagca
    12541 gcaacagtgt ttttgccagc aaccagctca gtactgcggg tgccaccaga cgcatattcg
    12601 cccaagtcag ccgcatactg ctacgcatcg aggcaaaaat gcccattttg tcctgtacca
    12661 gcatcaccgg tgccagggcc agtaaaatgg ccatgataat tcccggaacg accactagca
    12721 tgatgccaat ctgtactaaa agggtagtca gaaaaatcag aataaataac tttggcaata
    12781 tcggtgcact ggcaccaata gctcgcagcg cactgactct ctgacccgca gacaccagct
    12841 ggataattaa tattacgcct ccggcgagaa tggcgttacc gattaatcct gaaaaagtgg
    12901 acgccgctga agcctgcagc aaaatttgtt gctgttccgg tgacatattc tgaaccaggt
    12961 cgaacaaccc actactgccg ctaacgggca cgccgtcatt gagctgcgca agctgtgcat
    13021 cactgggtga gaaaacatgc cctaacacca ctgtgataaa cgcacatagc aacgatacca
    13081 acagaatggt cataaattga ttacggaaga aatttccggt gtcacggtat acggactgcg
    13141 ccgtgataga catgcactct ccttgagttt tatgcaggtg ttaattagcg ggcaattgta
    13201 ccctggttaa gcctcgggtg gcagcatcag gtcttgtatg gaaaagcata tctttgtaaa
    13261 gcggaggtaa ttgctggcct tcaggcgtgg gcaaaccgcg gcaagtggcg atcgagttaa
    13321 tatttgcgta gcgaaaatat ttaaaaattg atttaaatca cgttaaccag gattctcaat
    13381 gcaacttcta aattaatcca gatcaataaa gggtgaatta tcatatgtaa tgtgatctat
    13441 gtaggatcat ttgttactcc aatgtaggta tattcgtcac gtttttataa ccataacgac
    13501 ggagcggata tgaaaaagtt aacagtggcg gctttggcag taacaactct tctctctggc
    13561 agtgcctttg cgcatgaagc aggcgaattt tttatgcgtg caggttctgc aaccgtacgt
    13621 ccaacagaag gtgctggtgg tacgttagga agtctgggtg gattcagcgt gaccaataac
    13681 actcaactgg gcttgacgtt tacttatatg gcgaccgaca acattggtgt ggaattactg
    13741 gcagcgacgc cgttccgcca taaaatcggc acccgggcga ccggcgatat tgcaaccgtt
    13801 caccatctgc cgccaacact gatggcgcag tggtattttg gtgatgccag cagcaaattc
    13861 cgtccttacg ttggggcagg tattaactac accaccttct ttgataatgg atttaacgat
    13921 catggcaaag aggcagggct ttccgatctc agtctgaaag attcctgggg agctgccggg
    13981 caggtggggg ttgattatct gattaaccgt gactggttgg ttaacatgtc agtctggtac
    14041 atggatatcg ataccaccgc caattataag ctgggcggtg cacagcaaca tgatagcgta
    14101 cgcctcgatc cgtgggtgtt tatgttctca gcaggatatc gtttttaatt ccgcacaaaa
    14161 acgaccccgt aatatacggg gtcaataagg acatggca
//
LOCUS       DADLFA010000103        13911 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000103 DADLFA010000000
VERSION     DADLFA010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13911
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.103"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..510
                     /gene="tnpB"
                     /locus_tag="JQH65_002593"
     CDS             <1..510
                     /gene="tnpB"
                     /locus_tag="JQH65_002593"
                     /inference="COORDINATES: protein motif:HMM:NF013544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAY6139970.1"
                     /translation="SASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKKLARLQRQLS
                     RKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSKS
                     AAGTVSLPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWSGGQVLAVPPALRVLWSY
                     SERKSPVTK"
     gene            443..670
                     /locus_tag="JQH65_002594"
     CDS             443..670
                     /locus_tag="JQH65_002594"
                     /inference="COORDINATES: protein motif:HMM:NF018933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY6139971.1"
                     /translation="MLFRQRCACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNIL
                     AAGHAVLACGEMVQSGRPLKQEPTEMIQATA"
     gene            841..1188
                     /locus_tag="JQH65_002595"
     CDS             841..1188
                     /locus_tag="JQH65_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HAY6139972.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            1178..1540
                     /locus_tag="JQH65_002596"
     CDS             1178..1540
                     /locus_tag="JQH65_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HAY6139973.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            1537..2034
                     /locus_tag="JQH65_002597"
     CDS             1537..2034
                     /locus_tag="JQH65_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAY6139974.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGGENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(2042..3226)
                     /gene="emrD"
                     /locus_tag="JQH65_002598"
     CDS             complement(2042..3226)
                     /gene="emrD"
                     /locus_tag="JQH65_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HAY6139975.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCVGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFLFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(3645..3734)
                     /gene="tisB"
                     /locus_tag="JQH65_002599"
     CDS             complement(3645..3734)
                     /gene="tisB"
                     /locus_tag="JQH65_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HAY6139976.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            4299..4397
                     /gene="ivbL"
                     /locus_tag="JQH65_002600"
     CDS             4299..4397
                     /gene="ivbL"
                     /locus_tag="JQH65_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HAY6139977.1"
                     /translation="MTTSMFNAKLLPTALSAAVVVVRVVVVVGNAP"
     gene            4503..6191
                     /gene="ilvB"
                     /locus_tag="JQH65_002601"
     CDS             4503..6191
                     /gene="ilvB"
                     /locus_tag="JQH65_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HAY6139978.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            6195..6485
                     /locus_tag="JQH65_002602"
     CDS             6195..6485
                     /locus_tag="JQH65_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase isozyme 1 small subunit"
                     /protein_id="HAY6139979.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            6559..7149
                     /gene="uhpA"
                     /locus_tag="JQH65_002603"
     CDS             6559..7149
                     /gene="uhpA"
                     /locus_tag="JQH65_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HAY6139980.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            7149..8651
                     /gene="uhpB"
                     /locus_tag="JQH65_002604"
     CDS             7149..8651
                     /gene="uhpB"
                     /locus_tag="JQH65_002604"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HAY6139981.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            8661..9980
                     /gene="uhpC"
                     /locus_tag="JQH65_002605"
     CDS             8661..9980
                     /gene="uhpC"
                     /locus_tag="JQH65_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HAY6139982.1"
                     /translation="MLPFLKAPADAPLMTDKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            10118..11509
                     /gene="uhpT"
                     /locus_tag="JQH65_002606"
     CDS             10118..11509
                     /gene="uhpT"
                     /locus_tag="JQH65_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HAY6139983.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(11554..13320)
                     /gene="adeD"
                     /locus_tag="JQH65_002607"
     CDS             complement(11554..13320)
                     /gene="adeD"
                     /locus_tag="JQH65_002607"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAY6139984.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLDL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVYQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFVFTTLEVTE"
     gene            13495..>13911
                     /locus_tag="JQH65_002608"
     CDS             13495..>13911
                     /locus_tag="JQH65_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAY6139985.1"
                     /translation="MNNDNTDYVSNESGMLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMIS"
BASE COUNT         3129 a         3611 c         3873 g         3298 t
ORIGIN      
        1 tcagcatcaa tggtcgggct ggatgctggc gtggctaaac tcgccacgct gtcagatggc
       61 acagtctttg agcctgtaaa cagttttcag aaaaaccaga agaagctggc gagacttcag
      121 cgacagttaa gccgcaaggt caaattcagc aacaactggc agaagcagaa acgcaaaata
      181 cagcgactgc attcctgtat cgcaaatatc cgcagggact accttcacaa agtcacaacg
      241 accgtcagca aaaaccacgc aatgatagtc attgaggatt tgaaggtcag caacatgtca
      301 aagtcagcag cgggtacggt cagcctgccg gggcgcaatg tccgggcaaa atcaggttta
      361 aaccgttcga tactggatca gggctggtat gaaatgcgcc gccagcttga gtacaagcag
      421 ctctggagtg gcggtcaggt gcttgctgtt ccgccagcgt tgcgcgtgct gtggtcatac
      481 agcgaaagaa aatcgcctgt cacaaagtaa attcagatgc caggtatgtg gatatacagc
      541 gaacgccgat gtaaatggcg ctcgtaacat tttagcggcg gggcacgccg ttcttgcctg
      601 tggagagatg gtgcagtcag gccgcccgtt gaagcaggaa cccaccgaaa tgattcaggc
      661 gacagcctga acgtagcagg gatcctcgtc cttcagggcg tggaggatgt caatttgcgc
      721 tatgataccc atctgacctc cttgtagcgg atggcaaaag catatcagga aacgggcgga
      781 cgaaattctt aaaaaaaacc gctaaagata gagcgttagt gaaattgcaa aggagcaaca
      841 atgaaaattt cccgccttgg agaagcgccc gattaccgct tctcgctggc taatgagcgt
      901 acttttctgg cgtggatccg taccgcacta ggatttctgg cggcgggggt tgggcttgat
      961 caacttgcgc cagatttcgc cacgcccgtc attcgtgaac tgctggcgtt gttgttgtgt
     1021 ctgttttccg gcgggttagc aatgtatggc tacctgcgct ggttacgcaa tgaaaaagcg
     1081 atgcgtctga aagaggacct gccctacacc aacagcttat taatcattag cttaatattg
     1141 atggtcgtgg cggtgatcgt tatgggactg gtgttgtatg ccggatagcc gcaaagccag
     1201 acgcattgcc gacccaggac tgcagccgga gcgcacatca ctggcgtggt ttcgtaccat
     1261 gctgggctac ggcgcgttga tggcgttggc tatcaaacac aactggcacc aggcgggcat
     1321 gttattctgg atttcgattg gcattctcgc catcgtggcg ctgatcctct ggcactacac
     1381 ccgtaatcgc aatttaatgg atgtcacgaa tagcgatttt tcccaatttc acgtagtccg
     1441 tgacaaattt ttgatctccc tcgcggtgtt atctctcgca atactgtttg ctgtaacgca
     1501 tatacatcaa cttatcgtat ttattgagag agtcgcatga caggaagtca ggtcattgat
     1561 gctgaagagg acaggcataa actggttgta gagtacaagg atgctctgca acctgccgat
     1621 ttttatcaca atttcaaaca gcggggcatc cgctccgtac agctcattcc ctatcttgaa
     1681 tttgatgacc ggggcgatct gacggccgct tcggtgacag cagagctgtg ggggaaattt
     1741 ttaattgccc tgttcgagtg ttgggtgcgg gcagatatca gtcgtatctc gattgagctt
     1801 tttgacgcca ccttacaaaa atggtgtggc ggcgaaaacc cgcaaccgcg ccgtgactgc
     1861 caggcgtgcg actggcatcg cctgtgcccg cacgctcggc aggagacgcc agacagcgtg
     1921 ctttgtgcgg gctatcaggc gttttattct tactccgcac cgcatatgcg ggtgatgcgt
     1981 gatctgataa agcagcatcg ttctcccatg gagctgatga cgatgctgcg gtgacgtgcg
     2041 cttaaacggg ctgcccctga tgcgacatcc gcgtcgccag cggcagccag cacagcacga
     2101 tcaacaatcc cattaaggtc atcaataacc ccaggctacc ctgaccggtt tgcggcaaca
     2161 tcgcagagag cgacgccagc acgccggaac caatgttttg cagaccgccg accagcgcgc
     2221 cagccgtgcc cgccaggaag aggaacggct ccatcgcgcc gctggtcgcc agcggaaaca
     2281 gcatcccggc accgaaaaag aacagcgcgg cgggaacgag cagcgtccag acattcatca
     2341 cgccaaacca gtcggggatc cacatcagca agccagccag caggcagcag ataaccgact
     2401 gccacattaa cgtggagaag cgtttattgg gacgtccggc aaaccatgcg ccaaaaaacg
     2461 ctgccggaat cggcagaata aacaaaatac tgaccgtcat actgctcagc cctaacaccg
     2521 cgcccatcag cacgccggag caggcttcaa aggcggcaat cccggccaga ccgccaatca
     2581 gcatcagcaa ataacagtta aaaccgctgt taccgaaaag cgttttataa ctggtaagca
     2641 ggcgcgtgcg cggtgcatcg accggacgcg tttccggcat ccagcgggcc atactgaagg
     2701 tcacaccaac acaaagaacc aacaaaaaga gataacaggc gcgccagttc cacatcgtat
     2761 ccagcagacc gccgattagc ggtgcgagca acggactgac gagaatcccc atgtttaaca
     2821 ggctgttagc atggcgcaac tgtgtccgtt catataaatc tcgcggcaaa gtacgcgcca
     2881 ttacgccgcc aacgccggtg cccatcccct gcatcgcgct ggcggcaatc aataccgtca
     2941 aactggaggt cgtgaccgcg accagcgttg ccagcataaa aatggacatt ccgacgagga
     3001 tcaccggtcg gcgacccaca cggtcggaaa tcgggccata aaacagctgt gagacaccgt
     3061 aagtcagcag ataagcgccc attacgctct gcaccgcccc ttcacggacg ttgagatcgc
     3121 gcgccatatc ggcaatagct ggaatataaa tggtttgcgc catctgaccg acggccacga
     3181 gtaataccaa catcaataac aaattgacgt ttctttgcct tttcattatc acggatgctt
     3241 ttataaaaat aattagaata agtcactacg cattcccata aatgcgggag aggaatctat
     3301 cacattgatg aatgttagcc agatatacgc caggaatggg gaattgttta gcggcagaat
     3361 atgtaaacaa aagcggcaat aaatgttgcc gggagggatt acgtgcgaac tgtaaccctc
     3421 tccccaaagg ggcgaggggg aaaccaccgt tccctttcag aaaatgagct aaacattgca
     3481 cgggacggtc ccctcgcccc tttggggaga gggctagggt gaggggcaca ggcagcgcag
     3541 gtgcaaacat tctgctggta gaagaagccc ccaactccaa cccgcacgct aaatataaaa
     3601 ggggagcggt tacccgctcc cctttggtgc gacttgaatc tgaattactt caggtatttc
     3661 agaacagcat caagcagttg cagtgctgca acaatgagtt tgaggataag aatggcgata
     3721 tccaccaggc tcatacgcgt ctcctgtggt tcaggagcac tgccagctga cgtacctttc
     3781 cgctgcctgt tgccagctgt tgtgcacgcg gtatctcaag gagaaacgtt gtgcttgttg
     3841 acataacaca gtgtgctcac gcggggttaa ggcgctgacg gcaccacccg tttcagccag
     3901 gacttagtgc gccgggtaac gatgcggccc ccgcataata cattgcgtac agtgatatta
     3961 tagaagttta ctgtataaat aaacagtgat atttggacaa aacgcaaact gtgatcgata
     4021 ctcaactatg ccctaaatac gtcaaaattc gtgccgaaat tgcgcgttct gcgcggaaca
     4081 cgtatacttt cagtgttgac ataatacagt gtgctttgcg gttaccagct gcaggcggct
     4141 gatgaaacct cgctccggcg gggttttttg ttacctgcaa ttcagtacaa aacgtgatca
     4201 gcccctcaat tttcccttgc tgaaaaattt tccattgtct cccctgtaaa gctgtgcttg
     4261 tataaatatt gttaaacaca aaaccaacaa ggtccccaat gactacttcc atgttcaacg
     4321 caaaactact accaactgcg ctatccgccg cagtggtcgt cgtgcgtgtg gtggtggtcg
     4381 tcggcaatgc gccgtaggga ctggaacaac acacgattcc aaaaccccgc cggcgcaaac
     4441 cgggcggggt ttttcgttta agcacctccc ggaaagtcgg cccagaagaa aaggactgga
     4501 gcatggcaag ttcgggcaca acatcgacgc gtaagcgctt taccggcgca gaatttatcg
     4561 ttcatttcct ggaacagcag ggcattaaga ttgtgacggg cattccgggc ggttctatcc
     4621 tgcctgttta cgatgcctta agccaaagca cgcaaatccg ccatattctg gcccgtcatg
     4681 aacagggcgc gggatttatc gctcagggga tggcgcgcac cgacggtaaa ccggcggtct
     4741 gtatggcctg tagcggaccg ggtgcgacta acctggtgac cgccattgcc gatgcgcggc
     4801 tggactccat cccgctgatt tgcatcaccg gtcaggttcc cgcctcgatg atcggcaccg
     4861 acgccttcca ggaagtggac acctacggca tctctatccc catcaccaaa cacaactatc
     4921 tggtcagaca tatcgaagaa ctcccgcagg tcatgagcga tgccttccgc attgcgcaat
     4981 caggccgccc aggcccggtg tggatagaca ttcctaagga tgtgcaaacg gcagtttttg
     5041 agattgaaac acagcccgct atggcagaaa aagccgccgc ccccgccttt agtgaagaaa
     5101 gcattcgtga cgcagcggcg atgattaacg ctgccaaacg cccggtgctt tatctgggcg
     5161 gcggtgtgat caatgcgccc gcacgggtgc gtgaactggc ggagaaagcg caactgccta
     5221 ccaccatgac tttaatggcg ctgggcatgt tgccaaaagc gcatccgttg tcgctgggta
     5281 tgctggggat gcacggcgtg cgcagcacca actatatttt gcaggaggcg gatttgttga
     5341 tagtgctcgg tgcgcgtttt gatgaccggg cgattggcaa aaccgagcag ttctgtccga
     5401 atgccaaaat tattcatgtc gatatcgatc gcgcagagct gggtaaaatc aagcagccgc
     5461 atgtggcgat tcaggcggat gttgatgacg tgctggcgca attgatcccg caggtggaag
     5521 cgcaaccgcg tgcagagtgg caccagttgg tagcggattt gcagcgcgaa ttcccgtgtc
     5581 caatcccgaa agcgtgcgat ccattaagcc attacggcct gatcaacgcc gttgccgcct
     5641 gtgtcgatga caatgcgatt atcaccaccg atgtgggtca gcatcagatg tggaccgcgc
     5701 aagcttatcc gctcaatcgc ccacgccagt ggctgacctc cggtgggctg ggcacgatgg
     5761 gttttggcct gcctgcggcg attggcgcgg cgctggcgaa cccggatcgc aaagtgttgt
     5821 gtttctccgg cgacggcagc ctgatgatga atattcagga gatggcgacc gccagtgaaa
     5881 atcagctgga tgtcaaaatc attctgatga acaacgaagc gctggggctg gtgcatcagc
     5941 aacagagtct gttctacgag caaggcgttt ttgccgccac ctatccgggc aaaatcaact
     6001 ttatgcagat tgccgccgga ttcggcctcg aaacctgtga tttgaataac gaagccgatc
     6061 cgcaggctgc attgcaggaa atcatcaatc gccctggccc ggcgctgatc catgtgcgca
     6121 ttgatgccga agaaaaagtt tacccgatgg tgccgccagg tgcggcgaat actgaaatgg
     6181 tgggggaata agccatgcaa aacacaactc ataacaacgt aattctggag ctcaccgttc
     6241 gcaaccatcc gggcgtaatg acccacgttt gtggcctttt tgcccgccgc gcttttaacg
     6301 ttgaaggcat tctttgtctg ccgattcagg acagcgacaa aagccatatc tggctactgg
     6361 tcaatgacga ccagcgtctg gagcagatga taagccaaat cgataagctg gaagatgttg
     6421 tgaaagtgca gcgtaatcag tccgatccga cgatgtttaa caagatcgcg gtgttttttc
     6481 agtaaccgct caaggcttga acaacatcgc gcttatcgtt aaggtaagcg cgtttttttt
     6541 acccgccagg acaagaccat gatcaccgtt gcccttatag acgatcacct catcgtccgc
     6601 tccggctttg cgcagctgct ggggctggaa cctgatttgc aggtagttgc cgagtttggt
     6661 tcggggcgcg aggcgctggc ggggctgccg gggcgcggtg tgcaggtgtg tatttgcgat
     6721 atctccatgc ccgatatctc cggtctggag ctgctaagcc agctgccgaa aggtatggcg
     6781 acgattatgc tctccgttca cgacagtcct gcgctggttg agcaggcgct taacgcgggg
     6841 gcacgcggct ttctttccaa acgctgtagc ccggatgaac tcattgctgc ggtgcatacg
     6901 gttgccacgg gcggctgtta tctgacgccg gatattgcca ttaaactggc atccggtcgt
     6961 caggacccgc taaccaaacg tgaacgccag gtggcggaaa aactggcgca aggaatggcg
     7021 gtgaaagaga ttgccgccga actgggcttg tcaccgaaaa cggtacacgt ccatcgcgcc
     7081 aatctgatgg aaaaactggg cgtcagtaac gacgtagagc tggcgcgccg catgtttgat
     7141 ggctggtgat gaagacgttg ttctcccgct taattaccgt tattgcctgc ttttttatct
     7201 tctctgccgc atggttttgc ctgtggagta tcagcctgca tctggttgag cgccctgata
     7261 tggcggtgct gttatttccg tttggtctgc gtctggggct aatgctgcaa tgcccgcgcg
     7321 gatactggcc ggtgctgctg ggcgcggagt ggctgctgat ttactggcta acgcaggcgg
     7381 tcggtttaac ccattttccg ttattgatga tcggtagttt actgacgtta ctgcccgtag
     7441 cgctgatctc gcgctatcgc catcagcgtg actggcgcac cttgctgtta cagggggcgg
     7501 cgttaacggc ggcggcgttg ttgcagtcgc tgccctggct ttggcacggc aaagagtcgt
     7561 ggaatgcgct gttgctgact ttaactggcg gcctgacgct ggccccgata tgtctggtgt
     7621 tctggcacta tctcgccaat aacacctggc tgccgctcgg cccgtcactg gtttctcagc
     7681 caatcaactg gcgcgggcga catctggtct ggtacttgct gctgtttgtt atcagtctct
     7741 ggctccagtt gggattgccg gacgaactgt cgcgctttac gccattctgt ctggcgctgc
     7801 cgattatcgc gctggcctgg cactatggtt ggcaaggggc gctgattgcg acgttgatga
     7861 acgccatcgc gctgatcgcc agtcaaacct ggcgcgatca tccggtggat ttattgctct
     7921 cgctgctggt gcaaagtctg acagggttgt tgcttggcgc tggcatccag cggttgcgtg
     7981 aacttaacca gtcgctgcaa aaggaactgg cgcgcaatca gcatctggct gaacggttgc
     8041 tggaaaccga agagagcgtg cgccgtgatg tggcgcgtga gctgcatgat gatatcggtc
     8101 agaccatcac tgctattcgt actcaggcgg gcattgttca gcggctggcg gcagataacg
     8161 ccagcgtgaa gcagagcggg cagctcatcg aacaactatc gctgggcgtt tacgacgcgg
     8221 tgcgccgttt gttgggtcgg ttacgtccgc gccagttgga tgatctcacc ctggagcagg
     8281 ccatccgctc actgatgcgg gaaatggagc tggaagggcg cggtattgtc agccatctcg
     8341 aatggcgaat cgatgaatca gcgttaagcg aaaaccagcg cgtgacgctg tttcgtgtct
     8401 gccaggaagg gctgaacaac attgtgaaac atgctgatgc cagcgcggtc accctgcaag
     8461 gctggcagca ggatgaacgg ttgatgctgg ttattgaaga cgatggcagc ggtttgccgc
     8521 cgggttccgg gcaacaaggt tttggcctca ccggaatgcg cgagcgcgta acggcgctgg
     8581 gtggcacatt acacatttcc tgtctgcacg gcacgcgtgt cagcgtttct ctacctcaac
     8641 gctatgtcta aggtttgatg atgttgccgt ttctgaaagc gcctgccgat gcgccattaa
     8701 tgactgataa atatgaaatt gatgcccgct atcgctactg gcgtcggcat attctgctga
     8761 ccatctggct gggttacgcg ctgttttact tcacgcggaa aagttttaac gccgccgtac
     8821 cagaaatcct tgctaacggc gtgctcagcc gtagcgatat cggcctgtta gcgaccctgt
     8881 tttacattac ctatggcgtg tcgaagtttg tctccggcat tgtcagcgat cgctcaaatg
     8941 cccgttattt tatggggata gggcttatcg ccacgggcat tatcaacatt ctgtttggct
     9001 tctcgacgtc gctatgggcg tttgccgtac tctgggtgct gaacgccttt ttccagggct
     9061 ggggttcacc ggtgtgtgcg cgtctgttaa cggcctggta ttcacgtacc gagcgcggtg
     9121 gttggtgggc attatggaac acggcgcata acgtcggcgg cgcactcatt cccattgtga
     9181 tggcagcggc tgcactacat tacggctggc gtgccgggat gatgattgct ggttgtatgg
     9241 cgatcgtcgt gggaattttt ctctgctggc ggctgcgcga tcgcccgcag gcgttaggtt
     9301 taccggcggt cggtgaatgg cgacacgacg cgctggagat agctcaacaa caagaagggg
     9361 cagggctgac gcgtaaagag atcctcacca aatatgtgtt gctgaatccg tatatctggc
     9421 tgctttcgtt ttgctatgtg ctggtctatg tggtccgggc ggcgatcaac gactggggca
     9481 atttgtatat gtccgagacg ctgggcgtcg atctggtcac ggcgaatacg gcagtgacga
     9541 tgtttgaact gggtggattt atcggtgcgc tggtagcggg ttggggctcg gacaaattgt
     9601 ttaacggcaa ccgagggccg atgaatttga ttttcgccgc cggaattttg ctttcagtcg
     9661 gctccctgtg gctgatgcca tttgccagct acgtgatgca ggcaacctgc ttcttcacca
     9721 ttggtttttt tgtctttggc ccacagatgt taatcggtat ggcggcggca gagtgttccc
     9781 acaaagaggc ggcaggggcg gcgacggggt ttgtcggctt gtttgcttat ctgggggcgt
     9841 cgcttgctgg ttggccgctg gcgaaagtac tcgatacctg gcactggagc ggattttttg
     9901 tggttatctc tatcgccgcc gggatttccg cactgctgtt actgcccttt ttgaacgccc
     9961 agacaccgcg cgaagcgtga tgcatctcac cttttcactt catatccggc aaaactaaga
    10021 aattttccag gttttgcctg gacgctatct caggcctgat ttgctgctga tttttacaat
    10081 gcatgcctca cgcaggtatt catttcagga gtaacccatg ctggctttcc taaaccaggt
    10141 tcgcaagccg accctggacc ttccgctcga agtgcggcgc aaaatgtggt tcaaaccgtt
    10201 catgcaatcc tacctggtgg tctttatcgg ctacctgacg atgtacctga ttcgcaagaa
    10261 ctttaacatc gcgcagaacg atatgatttc gacctacggg ttgagcatga cgcagctggg
    10321 gatgatcggc ctgggtttct ccatcactta tggcgtgggt aaaacgttgg tttcatacta
    10381 cgccgacggc aaaaacacca aacaattcct gccgttcatg ctgatcctgt ctgccatttg
    10441 tatgctgggc ttcagtgcca gtatgggcag cggctcggtt agcctgttcc tgatgattgc
    10501 cttctacgcc ttaagcggct ttttccagag taccggcggt tcgtgcagtt actccaccat
    10561 caccaaatgg acgccgcgtc gtaaacgcgg gacattcctc ggtttctgga atatttctca
    10621 caaccttggc ggtgcaggcg cagcaggtgt ggcgctgttc ggggcaaatt acctgttcga
    10681 tggccatgtc atcggcatgt ttatcttccc gtcgattatc gcgctgattg tcggttttat
    10741 cggcctgcgt tacggcagcg actccccaga atcttatggc ctcggcaaag ctgaagaact
    10801 gttcggcgag gagatcagcg aagaggacaa agagacagaa tctaccgata tgaccaagtg
    10861 gcagatcttt gttgagtatg tgctgaaaaa caaagtgatc tggctgctgt gcttcgccaa
    10921 cattttcctc tatgtggtac gtattggtat cgaccagtgg tcaaccgtat acgcgttcca
    10981 ggaactgaaa ctctctaaag cggtggcgat tcagggcttt acgctgtttg aagctggtgc
    11041 gctggtcggt acgctgctgt ggggctggct ctctgacctg gcgaacggtc gccgtggcct
    11101 ggtggcctgc atcgcgctgg cgctgattat cgccacgctc ggtgtgtatc aacatgccag
    11161 caacgaatat atctatctgg cttctctctt tgcgttgggt ttcctggtct ttggcccgca
    11221 attgttgatt ggtgtggctg ctgttggctt tgtacctaaa aaagcgattg gcgctgccga
    11281 tggtattaaa ggcacctttg cttacctgat tggtgacagc tttgccaagt taggtctggg
    11341 aatgattgcc gatgggacgc cggtattcgg ccttaccggc tgggcaggca ccttcgccgc
    11401 acttgatatc gccgcgattg gttgtatctg cctgatggcg atagtggcgg tgatggaaga
    11461 acgcaaaatc cgccgcgaga aaaaaattca gcagttgaca gtggcataaa cgtaactggt
    11521 gacttttgcc cggcatgacg ccgggctttt ttattattcc gtgacttcca gcgtagtgaa
    11581 gacaaacttc tcgccatcaa atagcccctg actggttagt ttgagcgcgg ggatcactgg
    11641 cagagaaaga aacgccatct gaataaacgg ctcatcgggt aacggaccgc attcacgggc
    11701 ggcggctttc aaggcgtcaa tttgttccgc cagcgactgc gccgtgtcgg tgctcatcag
    11761 gccggcaatg ggcaacggca gatgactttg gacctggccg ttacgtacca cgcacagccc
    11821 gccgccatcc tgaatcacct gatagaccgc cagcgccatc tcttcggcac tgcgaccgat
    11881 caccacaata ttatggctgt catggctgac cgtcgcagcc agcgcacctt cattcagtcc
    11941 aaagccgcca agcaaaccac aagccggagc cagccgttgc ccgtaacgct caagtacggc
    12001 aataaagcac acatcatcgc gatcaaaacc attttcgctg tagacgctgg agtgggagtg
    12061 cgtaatcaat tcgttatgga tgacgtcaat gacccgatag cgttttccgg gcgtaaattg
    12121 cagggcaaag tcgctggcgg aaactggctg gcgggcaatg gtgttgccat atggcggagc
    12181 ggattgtgcc agtctcgccg actcttccgc ctgtaaggtt tgcgcatcaa tcggctcgcc
    12241 tttcaccagt acctgctgca ccgtgacctt acgcgcatcg ctcaacagga cgatatcggc
    12301 ctgcttgccg ggtgccagta agtcgaggtg attcagacca aagtggcgcg ccgtcgacca
    12361 gctggcgacg cgatatgcca catgcagcgg cacattgtgt tgttcgatca ggcggcgaat
    12421 taaggcatcg atgtgtcctt catgggcgat ctcccacggg ttacggtcat cggtacagag
    12481 catgcattgc gggctgttaa attcgttgat caacggtgcc agcgcgttga gattgcgggc
    12541 agcggaccct tcgcggatca tcaacgacat gccgagttgt aatttccggc gtccttcttc
    12601 cagctgataa ctttcgtggc agttttcaat acccgcagta atataggcgt taagttcttt
    12661 accacccaaa cccgggcagt gaccgtccag cgtcaggtgg cgaaatgcat ccagtttatc
    12721 gagcagcgca ttctgcccgc taattacgcc agggtagtcc atcatttctg caaggccggt
    12781 aacctgcgga tggtcccgcc aggcgagcat ctgttcaagg gtaaaactgg caccgttaac
    12841 atcgcagcct tccagggcgg gtacgcaaga gctgacctgt aagtactggt tttgccttgc
    12901 ctgttcggca cagcgggcaa accaggcgaa tccggcttct cccatcacgt tgacgatttc
    12961 atgagggtcg caaataacgg tcgtcaggcc gcgcggcagg gtagcggttt caaaggtgac
    13021 cggcgtcatc atgctggatt caatatgcag gtgagcatca ataaaccctg gcaccgccgt
    13081 tgcgccgcga gcatcaatcc gctgcaaagc cggagcatca gcgtattctg cgccaacacc
    13141 ggcaatgtaa cgtcctttaa tcacaattgg gccggaaatt tctccgccat tgatcaggtc
    13201 gagaatagag acattatcaa taatataatc ggcaacagcg tcgccacggg aaacggctaa
    13261 caattcctgg tattcagccc ggctaatgtg atgaaattta tggttaatgg aattattcat
    13321 atttttctca ttttgatttt atcaatttga ttattattta tcataaagaa acgtcgcgag
    13381 attattaaag aatatctatt aatgtgcaat tgaaatgtga taattatcac tgaaccgttg
    13441 ttgtcatttg ttttacaaaa gcaagggatt aaatctcctc caatggacaa aaaaatgaat
    13501 aatgacaata ccgattacgt gagtaatgaa tcagggatgc tttcgcgatt atttaaacta
    13561 cctcagcatg ggaccaccgt ccgcacagaa ttgattgcgg ggatgaccac ttttttaacc
    13621 atggtgtaca tcgtttttgt gaacccgcaa atcctcggcg cggcacaaat ggacccgaaa
    13681 gtggtgtttg ttaccacctg tttgattgcc ggtatcggca gtattgcgat ggggatattt
    13741 gctaacttac ccgtggcgct ggctccggca atggggctga acgccttctt tgccttcgtg
    13801 gtcgtggggg cgatgggcat ctcctggcag accgggatgg gcgcaatatt ctggggcgca
    13861 gttggactat ttttgctcac gctgtttcgt atccggtact ggatgatctc c
//
LOCUS       DADLFA010000104        13818 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000104 DADLFA010000000
VERSION     DADLFA010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13818
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.104"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(112..2038)
                     /locus_tag="JQH65_002609"
                     /pseudo
     CDS             complement(112..2038)
                     /locus_tag="JQH65_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017264.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
     gene            2445..3143
                     /locus_tag="JQH65_002610"
     CDS             2445..3143
                     /locus_tag="JQH65_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAY6139986.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRISMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPTHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWFA"
     gene            3249..3324
                     /locus_tag="JQH65_002611"
     tRNA            3249..3324
                     /locus_tag="JQH65_002611"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3282..3284,aa:Phe,seq:gaa)
     gene            complement(3473..4009)
                     /locus_tag="JQH65_002612"
     CDS             complement(3473..4009)
                     /locus_tag="JQH65_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAY6139987.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLAQHLAGRSPREKGMLLAAV
                     VFLFSVGYYVLIWQPLSERIVQQETMLQQLVAMNTRLRSAAPDIIAARKSATTTPAQV
                     SRVISDSASAHSVVIKRIAERGENIQVWIDPVVFNDLLNWLNALDEKYALRVTQIDAS
                     AAEKTGMVNVQRLEFGRG"
     gene            complement(4011..5189)
                     /gene="gspL"
                     /locus_tag="JQH65_002613"
     CDS             complement(4011..5189)
                     /gene="gspL"
                     /locus_tag="JQH65_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HAY6139988.1"
                     /translation="MSSILEIFFPLCAADPIHWQRRTPDVEHGIWSDVANEQLQQWLQ
                     TDAIRLYIPGEWISVWQVELPDVARKQIPTILPALLEEELNQDIDELHFAPLNIDQQL
                     ATVAVIHQQHMRNIAQWLQENGITRATVAPDWMSIPCGVMAGDAQRVICRIDECRGWS
                     AGRALAPVMFRAQLNEQDLPLSLTVVGIAPEKLSAWAGADAERLTVTALPAVTTYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSRTQA
                     EEQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLSDIEM
                     RGFTFDQKRQILHLQLRAANFASFDKLRSALATDYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            complement(5186..6163)
                     /gene="gspK"
                     /locus_tag="JQH65_002614"
     CDS             complement(5186..6163)
                     /gene="gspK"
                     /locus_tag="JQH65_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HAY6139989.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPLAVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMIRQQININ
                     TLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            complement(6166..6765)
                     /gene="gspJ"
                     /locus_tag="JQH65_002615"
     CDS             complement(6166..6765)
                     /gene="gspJ"
                     /locus_tag="JQH65_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HAY6139990.1"
                     /translation="MRRTRAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVADH
                     DQKLNLMQQTMSFLTHDLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHDGYLERLAWPLTDAAGSVKPTMQKLIPADSLRLQFYDG
                     TRWQESWSSVQAIPVAVRMTLHSPQWGEIERIWLLRGPQ"
     gene            complement(6762..7133)
                     /gene="gspI"
                     /locus_tag="JQH65_002616"
     CDS             complement(6762..7133)
                     /gene="gspI"
                     /locus_tag="JQH65_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HAY6139991.1"
                     /translation="MKRGFTLLEVMLVLAIFALAATAVLQIASGALSNQHVLEEKTVA
                     GWVAENQTALLYLMTREQRAVRHQGESDMAGSRWYWRTIPLNTGNALLQAVDIEVSRH
                     EDFSSVIQSRRAWFSAVGGQQ"
     gene            complement(7193..7693)
                     /gene="gspH"
                     /locus_tag="JQH65_002617"
     CDS             complement(7193..7693)
                     /gene="gspH"
                     /locus_tag="JQH65_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HAY6139992.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATDSEPPAKKAAQDF
                     LTRFAQFKDRAVIEGQTLGVLIDPPGYQFMQRRQGQWLPVSATRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYS
                     AAQNAC"
     gene            complement(7697..8152)
                     /gene="gspG"
                     /locus_tag="JQH65_002618"
     CDS             complement(7697..8152)
                     /gene="gspG"
                     /locus_tag="JQH65_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HAY6139993.1"
                     /translation="MNSLSRTHKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     NRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADSRNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            complement(8169..9392)
                     /gene="gspF"
                     /locus_tag="JQH65_002619"
     CDS             complement(8169..9392)
                     /gene="gspF"
                     /locus_tag="JQH65_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HAY6139994.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEA
                     RMNASAGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVMLVVATGVVTILLTAVVPKIIEQFDHLGHALPSSTRALIAM
                     SDALQASGVYWLAGLLALLVLGQWLLKNPTMRLRWDKTLLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFPP
                     MMLYIIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            complement(9392..10884)
                     /gene="gspE"
                     /locus_tag="JQH65_002620"
                     /pseudo
     CDS             complement(9392..10884)
                     /gene="gspE"
                     /locus_tag="JQH65_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
     gene            complement(10884..12944)
                     /gene="gspD"
                     /locus_tag="JQH65_002621"
                     /pseudo
     CDS             complement(10884..12944)
                     /gene="gspD"
                     /locus_tag="JQH65_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
     gene            complement(12974..13803)
                     /gene="gspC"
                     /locus_tag="JQH65_002622"
                     /pseudo
     CDS             complement(12974..13803)
                     /gene="gspC"
                     /locus_tag="JQH65_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238556.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
BASE COUNT         3112 a         3975 c         3559 g         3172 t
ORIGIN      
        1 ctgattcgcg acgtaagaaa gtggcaggct catatgacgg acctctgtaa tgcggtatcc
       61 aacccgcgaa tatcagcgtg attcaccgtc ggatttactg ctacgtcgcc ctcagggaat
      121 taaacctaaa aagaaaatcg tcaaaattag agattccggt ttgacacgga aagtcatatc
      181 agcgctatgc gcatcctggg gggtcatcac cactgacggg aaactctgct ggcggaacat
      241 cgctttttgt aggtctttga cgtcgaaact gacatacaga tcgtgcatct cctgacacaa
      301 ctggcgcaaa gtgtagtcgc gatagcgcac cggatatttg ttataaacgc tcggcaacac
      361 ctcagccagc ggcgagtcat cctcaatatg ctgttcaaac tgcgccaaca tctctaccag
      421 ttgtgccagc ttctcgtggc tttccgccgg agttaataag aacagaatgg agttgagatc
      481 gcacttctcc ggcacaatgc cgttctcacg cagatagtgc gccagaatcg tcgccggaac
      541 gccaaagtcg ctatattcgc cggtttcggc attgatacct ggtgtagtga gtaacagctt
      601 gcacggatca acaaaatact gatccgcggc atatccttca aagccgtgcc acttcgcccc
      661 cggctcaaaa ctgaaaaaac ggcggtcgct ggctaacact gatgtcggat aatcctgcca
      721 caatttgcca tcaacaacgg gcgggataaa cgggcggaac agcttacagc gcgcaagaat
      781 agccttgcgc gactcaatac ccaactcaac gcactcagcc cacagccgac gcccactctc
      841 cccttcatga attttggcgt taacatccag tgcagcaaac agcggataga aagggctggt
      901 agaagcatgg agcataaagg cgttattcaa ccgcttatgc gggcaaaaac gcgcctgtcc
      961 gcggatatgg ttatcttttt tatggatctg cgacgtctgt gagaatcccg cctgctgttt
     1021 gtgcaccgac tgagtcacaa agatccccgg atcgttttcg ttaagttcta atagcagcgg
     1081 cgagctatcc gccatcatcg ggataaactg ttcgtaaccg acccacgcgg aatcaaacag
     1141 aatgtaatca cacagatgcc caacggtatc gatcacctga cgggcgttat agacagttcc
     1201 gtcataggtt cccagctgaa taatcgccag gcgaaacggg cgcggcagct cggctttttc
     1261 tggcgcaacg tcgcgaattt gctggcgcag atactcttca ttaaaacagt gcgcatcaat
     1321 accgccaatg aaaccaaacg ggttgcgtga agcttccaga tagaccggcg tcgcccccgc
     1381 ctgaatcagc gcgccgtgat gattcgactt atggttgtta cggtcgaaga gcaccagatc
     1441 gccacgcgtt aacagcgcat tcgtcaccac tttattcgct gccgatgtgc cgttcagcac
     1501 aaaataggtt ttatcggcat gaaagacttt ggctgcgaat ttctgcgcat ctttcgccaa
     1561 taagcagatc gcccaatttt acgtcagcgt tacacatatc ggcgcgaaag acgttctcac
     1621 caaagaaatc gtaaaaatgg cgtccggcag gatgcttttt aaaaaacgca ccatgttgat
     1681 gtccagggca agcaaaggtg ctgttgccca tctcaacgta ctgcgtcagc gtgtcataaa
     1741 acggtggcag caaattctct tcatactgac aggctgcgga ttccagctcc agccactgct
     1801 gctcgttgcc gttgattacc gccgtaacgc ccgcaggtaa ttcaacagca tgttcggaat
     1861 acaaaaacac cggtagatga aaaccggtgc gcttaagcaa cgcaagaatg ccactgcgac
     1921 tatccgcagc ggtaatgacg actgccacga cgtccgtaaa atcagtatct cccaacgcca
     1981 ccacgcgacg atgagaagaa agtcgggata ccagttcact actggcggca atattcattg
     2041 atttcataag cgcaaacccg tttcggggaa gtaagaatac cggacaaggt ggaaaaccct
     2101 gccccatgag atatgggtca aactggtcac cagctccgac cgccagacat cagtaaaagc
     2161 agaaacgctc tgattttact gttgtcctgc agtgagcgtg cgttaacttc accgcatgag
     2221 cagtaacata gaaagggaaa cgtttcgcgc gaaacggcga taagcgagag aatgtaagga
     2281 ggtggcgtgc atcgggcaaa ctccgtacag agaggagaaa attcgcgcaa tcatggcacc
     2341 tttcgctaaa gcgtgcaagc caggactttg cgaccagaca agccatcaac aaggcaaaaa
     2401 gttcataata agaaaatcaa acaatacagt taacaggaac tcttgtggtc atcggccctt
     2461 ttatcaacgc tagtgcagtc ttactgggtg gcgttctcgg cgcactgctc agccaacgct
     2521 taccggaacg tatccgcatc tccatgacat ctatttttgg tctggcatcg ctggggattg
     2581 gtattttact ggttgtgaaa tgcgccaacc ttccagcgat ggttttagct acgctacttg
     2641 gcgctctaat tggcgaaatt tgtttgctgg agaaaggtgt caatacagca gtcgccaaag
     2701 cacaaaatct gtttcgtcac tcacgtaaga agccaacgca tgaatctttt attcagaatt
     2761 atgtcgcgat tattgtcctg ttttgcgcca gcggcaccgg gatcttcggg gcgatgaacg
     2821 aagggatgac cggcgatccg agtattttaa tcgccaagtc atttcttgat ttctttacgg
     2881 cgatgatctt cgcctgctcg ctgggtattg cggtgtcggt aattagtatc ccattactga
     2941 tcatccagtt aacgctggcg tgggctgccg cgctgatatt accgctgacc acaccgtcga
     3001 tgatggcaga cttcagcgcc gtaggcggtt tattgctgct ggcaaccgga ttacgcatct
     3061 gtggcattaa aatgttcccg gtggtcaaca tgcttccagc actccttctg gcaatgccgc
     3121 tttccgccgc ctggtttgcc tgacaatgcg tgcaatagcg gcaaagtgat gatagattgt
     3181 gcagtctgca gtaaattgaa gaaatttgat tgacgagacg aggcgaatca ggtttaatgc
     3241 gccccgttgc ccggatagct cagtcggtag agcaggggat tgaaaatccc cgtgtccttg
     3301 gttcgattcc gagtccgggc accactaatt cttaagaacc cgcccacaag gcgggttttt
     3361 gcttttggat ctgacaataa ccttcacgaa aaaagtttgc ttataaagtc tgggggaatt
     3421 actctcgcca cgttaacgag agtaatttta ttgatactaa tctcctgata ctttaccccc
     3481 gtccaaactc cagccgctgg acattcacca tcccagtctt ctcagcagca ctggcatcaa
     3541 tttgtgtcac ccgcagcgca tacttttcat ccagcgcgtt taaccagttc agcaggtcat
     3601 taaacaccac cggatctatc cagacctgaa tattctcccc acgctcggct atccgcttga
     3661 tgactaccga gtgcgcggaa gcactgtcac tgataacccg cgatacctgc gcaggcgttg
     3721 ttgtggcgga ttttcgcgcc gcaataatat ccggcgcggc gctcctcagt ctggtgttca
     3781 tcgccaccag ttgctgcaac attgtctcct gttgtactat ccgttcgctc aacggctgcc
     3841 agatgaggac gtaatatccg acgctaaaca ggaacaccac cgctgccagt aacatgccct
     3901 tttcacgcgg agaacgcccc gccaggtgtt gtgccagcca gtgttcgcca cggcttaact
     3961 ggcgttcacg ccattgctga aaatagtgaa taaatttatc gcgtaacatg ttatttcctc
     4021 cgcaacgtta cgccgccgga aaccgcatca ccctctttct gtaacgcgtc ctgttgcaca
     4081 acataatcgg ttgccagtgc actacgcagt ttgtcgaaac tggcaaagtt cgcggcccgt
     4141 agctggaggt gaagtatctg gcgtttttga tcaaaggtaa aaccacgcat ttcgatgtcg
     4201 gaaagtgacg ctgatttcag ggtgctggct atcgctgaca actcggcgag cagccgggta
     4261 tcgtcggcct gtgggcgata ttttttcagc gccatcgtca cctgagagcg taaattcaca
     4321 atccgcttct gctccgggaa caacgttaag aactgttcct ccgcctgggt gcggctttgc
     4381 gccacctgtt cgctgacgct ccataacgtc acgccccgct ccactgccag cgcaactaga
     4441 atcagcaata tcggcagaat catcacccgc cagcgcgccc actgttttcg gtagctgacg
     4501 cgaggctgcc acggccctgt cagcaggttc ccttccggtt cgccataagt ggtaacggcg
     4561 ggcagagctg taacggtcag gcgttcagcg tctgcgccag cccatgctga tagcttttcc
     4621 ggtgcaatgc cgactacggt tagcgaaagc ggtaaatcct gctcattgag ctgggcgcgg
     4681 aacatgaccg gagccagcgc ccgcccggcg ctccatcccc ggcattcatc aatgcggcag
     4741 ataacccgtt gcgcatcgcc agccataacc ccacaaggaa tggacatcca gtctggcgcg
     4801 acggtagcgc gggtgatgcc gttttcctgc aaccactgcg caatgttgcg catatgctgt
     4861 tggtgaatca ctgctacggt tgccagttgc tggtcgatat tcaacggggc gaaatgcagt
     4921 tcatcgatat cctggttcag ctcttcttcc agtaaggcgg gcagaatggt cggtatctgc
     4981 ttacgggcga catcaggcag ttcaacctgc cagacgctga tccattcgcc gggaatgtag
     5041 agtcgaatcg catcggtttg cagccattgc tggagctgtt cgttagcaac gtcagaccag
     5101 ataccgtgct ccacatcggg tgtacggcgc tgccaatgga tgggatcagc ggcgcaaagc
     5161 gggaaaaaaa tctcaaggat ggaactcact cactttctcc tgtctgatgc cagagaaccg
     5221 aaaagtgttg tgggcccatg cggacaatta acgagttcat cgtcagttca atctcattca
     5281 cggtgatatc tgaacgcagc cagaagtaat tgctgtccac gctcaggacg gtttttagct
     5341 gttttttagt acgctcatcg acgtcagcaa gtagcggctg tgcaagaaac tgatcgacat
     5401 cttcccagcc cttcgccgga cgttgttgta ataacgcccg cgcctgaacg gggcttaacc
     5461 acgggtcaaa cagcgcctca agaatcacac tttgcgtgac gtctaaagta ttgatgttga
     5521 tttgctggcg gatcatcggc agcgcacaga ccagcggttt cagtttttga taaagcccgg
     5581 cgtccattcc ctgtaccacg cgcatctcac tgatatcagc cagcggttga ttggcggcgt
     5641 agaacggcac cgaacgggcg agatactcgc tgtcttcacg gcccagacgt gtctgcacgc
     5701 tgcggtcctc gtcaataaac tcccacaggc tttcggctat cagttcggcc cgataagcag
     5761 gcacatccag gcgcgagatc agggcaatca actgttgtac cgcaagcgga cgcgacgccg
     5821 ttgtcggctg agcaagggca ttcaggttaa agcaagcctg tgcgtcacgc agggtgaccg
     5881 caatttgccc ctgcggcaga gggaagaaac gagggccgga tgcccacggc tgtgccagat
     5941 gcacgcgctt ttcatttttc aggctcagac tgagcgcgct cagcgccagg ctttccgcac
     6001 tggcgctgta ccacagcgcc tgctggtact cctgctggct gcgcgttcgc ccaagttgtt
     6061 gctgcattcg cccggaaagc gtgatggtta ccagcatcat aaccgccagc aataccagca
     6121 ccacgaccag tgccattccg cgttttggtg gtgaggtgat catgattatt gcggcccgcg
     6181 taacaaccag atgcgttcaa tctcgcccca ttgcggcgaa tgcagggtca tgcgtaccgc
     6241 cacagggatc gcctgtactg acgaccagct ctcctgccag cgcgtgccgt cgtagaactg
     6301 caaacggagc gaatcggccg gaatcaactt ttgcattgtc ggcttcacgc tgcctgcggc
     6361 atcggtcagc ggccaggcta accgttcgag ataaccgtca tgaatgcggt aaccgacggt
     6421 gagcagattg ctgcgcggca gacgcatcaa cgggttaacc acaccgccgc gcacaaaacg
     6481 cattccttca ctctcagacg ccagcacgcc agcgccagcc agtaaagctg gttcacgctg
     6541 gccctgatcg cctcttaccg gacgcggcat catttgggtc agatcgtggg tcagaaaact
     6601 catcgtttgc tgcatgaggt ttagtttttg atcgtgatcg gcgacggcgc tattcacgcg
     6661 cgtgacgccg ttagtcacct gctgcgccat cagtgccagc gaggcaaaaa tggcaattgc
     6721 caccaacatt tccagtaacg tgaaaccagc gcgagtcctt ctcactgttg gcctcccacg
     6781 gcgctaaacc aggcgcgtcg tgactgaatc accgacgaaa agtcttcgtg acggctgact
     6841 tcaatatcca ccgcctggag cagcgcgttg cctgtattca atgggattgt tcgccagtac
     6901 cagcggcttc ctgccatatc gctctcgccc tgatgcctga ccgcccgctg ctcacgggtc
     6961 atcaggtaga gcagcgcagt ctggttttca gctacccagc ccgctaccgt tttttcctca
     7021 agaacgtgct gattactcag cgcaccgctg gcaatctgta acaccgccgt ggcagccagc
     7081 gcaaaaatcg ccagcacgag catcacttcc agcaaggtaa atccgcgctt catggcatcc
     7141 tctcatcaca ttgattgagg gataacgcgc catcgtgtgc cagctttacc gctcaacatg
     7201 cgttttgcgc cgccgagtag aagcgcagcg taaacggcgt ggcgggttca aaaggcgaaa
     7261 aacggatctg cggcgtcttc tttttcgcct ctttttgcaa ctccagttca atatcgtgca
     7321 gcgtcaggcg acgacgttgc agctccagcg catattcctt ctgccagata tcacttccgg
     7381 gttgtaacag catctgcacc tgtttcggca ccgtaacctg tgccgataac cgggtcgcag
     7441 aaacgggcaa ccactgcccc tgacgacgct gcataaactg atagccaggc gggtcgatta
     7501 gcacaccgag tgtctgtcct tcaatcactg ccctgtcctt aaactgcgca aagcgagtca
     7561 gaaaatcctg tgccgctttt ttcgcgggcg gttcagaatc ggtcgcaaac gtctgtacca
     7621 cgcccgcact ggcaaggccg ataaggaaaa tcaccagcat gatttccaga agtgtgaatc
     7681 cgcgttcagg cactgcttac tgaaactcct gcaaattcca gttaccgata tctgcgccag
     7741 cgccttcccc gttttcctga ccatccgccc ccagggtata aacatcaaac agtccttttt
     7801 cacccgggct gagatactga taatcattgc cccacggatc ctttggcagt cgtttgatgt
     7861 atccaccagt acggtagttg cgggagtccg ccatattggc tggctgctgg atcagcgctt
     7921 ccagcccctg ttctgtggtc ggataacgcc cgttatccag tcggtacata tctagtgcgt
     7981 tttccagcgc cacgatatcg ctgatggctt tttgccgatt ggctttctct ttgttgccca
     8041 acaggttagg caccaccaga cttgccagga cgccaagaat aacaatcacc accatcactt
     8101 ccagcagggt aaaacctgcc cgtggtttgt gtgtgcggga taacgaattc atgtgataac
     8161 tccgtaaatt acattccaac catattattc agttgcagca tcggctcgag gatggcgatg
     8221 acaataaaca gcaccacgcc cgccatcatc accaccagcg caggctcaaa cagccccaac
     8281 gccagcccca cctgggtatc aaattcccgt tcctggttaa cagcggcctg ctcaagcatg
     8341 gtttccagtt cgccgctctg ttcgccggaa gcgataatgt acagcatcat cggcgggaac
     8401 aggcgcaact ccgccagcgc ggcgcgcaga ctgcttcctt cgcggacgcg atctgccgcc
     8461 agcagcagtt gttgttcgac atagcgattt gccgacacgg cggcagcggt ctgaatgcct
     8521 tccagtagcg gaacactgct ggcggtgagg atgcttaatg tgcgggaaaa acgcgccgta
     8581 ttcagcccgc gcgcaacacg gcccgtcacg ggcagacgca gcaaggtttt atcccagcgc
     8641 aggcgcatag taggattttt aagtagccat tgccccagca ccagaagcgc cagcaatccc
     8701 gccagccagt aaacgccgct ggcctgtaac gcatcactca tagcgataag cgcacgggtg
     8761 gaggagggta gcgcgtgtcc gagatgatca aactgttcga tgattttcgg caccactgcc
     8821 gtcagcaaaa tagtgaccac gcccgttgcc accaccagca taaccagcgg atagagcatg
     8881 gcctgcaata gacgtgattt caggcgctgt cgctgttcgg tgtaatctgc caggcgattg
     8941 agcaccacgt cgagatgccc ggatttttct ccggcagcga ccatcgaaca aaacagggaa
     9001 tcaaagacgc ggggatgttc gcgcaggctg tccgacaggg tataaccttc ctgaatccgg
     9061 ctgcgcagcg ccattccgag gctcttcaca tgcagttttt cactttgctc actgaccgcc
     9121 tgcaagcagg tttccaacgg cattgctgcc tgcaccagcg ttgccagttg gcgcgtgaac
     9181 agcgccagat ctgccgccgc cacgcgacga tgtgcgtgcc gccgacgctg caacattccc
     9241 cctgccgatg cattcatcct ggcttcaatg tgcacgggga taagctcttt accgcgcaac
     9301 aactggcggg catgacgcgc ggaatccgcc tcaatcatgc ctttggtttt gcgaccatta
     9361 cgctccagcg cctgatagta aaacagtgcc attacgcctc catggttacc cgcagaactt
     9421 catcgagaga ggtttctccg gcgagcactt tctcaatgcc gttgctgcgg atacccgcag
     9481 agtgttgccg gacataacgt tccagctcca gctccccggc ctgacggtgg atcaaatcac
     9541 gcaatgtggc atccaccacg tcagctcatg gatggcagtc cgtccgcgga aacctttgtg
     9601 attacaggcg gggcagccct gcggatggta cagagtgacg gttcgcgcat cggtaatgcc
     9661 cagcaggcgt ttttcttcgt cagtggcagg cgcggcctga cggcagtcgg gacacagcgt
     9721 gcggaccagt cgctgcgcca tcacgcccgt cagactggaa gagagcagga aaggctccac
     9781 gcccatatcc tgcaaacgcg tgatcgcccc caccgccgtg ttggtatgca gcgtggaaag
     9841 gaccaggtgt ccggtaagcg atgcctgaac agcgatttct gcggtttcgg tatcgcggat
     9901 ttcaccgacc atcaccacat ccgggtcctg acgcaaaatc gcgcgcaggc cacgggcgaa
     9961 ggtcatgccg acgcgggtgt taacctgtgt ctgaccgatc ccttcaatca tgtattcgat
    10021 ggggtcttca accgtgagaa tgttacgcga atggttgttc agctcctgca atccggcgta
    10081 cagcgtggtg cttttgccgg aacccgtcgg ccccgtcacc agaaagatgc cgtgcggttt
    10141 gtgtaacagc tggcgcaact gcacggtcag ttgctggcta agccccagac gctccagcgt
    10201 caggcgggcc tggtttttgt ccagcagtcg cagcaccacg cgctcgcccc aggcagaagg
    10261 catggtggag acacgcacgt caatcgcccg gccgcccagc agcagcgcaa tacgtccatc
    10321 ctgcggtacg cgcttttcgg cgatatccag ccgtgccatc accttgatac gcgataccag
    10381 cagcgaggcc agtttgcgcc ccgggcgcag catttcatgt aatgtgccgt caacgcgaaa
    10441 acggatcacc agactctttt caaacgtctc gatgtggata tccgaagcgc cttctttaat
    10501 cgcctctgcc agcatggcgt tgatcagttt gatgatcggc gcatcgtcgt cactttccag
    10561 cagatcttcc gtttctggca gttcttccgc gagagtaaaa aagtcctcag cagaaccgag
    10621 atcttccatc agctggcgag cctcggaaga gtcccgctgc cagaccgcat tcagccgctg
    10681 ttcaaattcg gcctcgtcga tttgccgcag cgtaaagggc gcgttcagcc cccgctgcag
    10741 ctcctgcaaa acagagagcg atagcggatg aacgtggagg atctccagcg acgcttcgca
    10801 ccatgccacc aggctaaacc gacggctgaa actgtagggc agacgcacgg tcttggcggt
    10861 ggtttcctgt gctacaggca ccattaacgc gttctcccgg cattgaggaa cgcgcgaact
    10921 tccggcggta aggcctggtt ttgcgctggc agtaccggct gtgcggtgtg cggcatcagg
    10981 cttaaacctt gctcatcacg atagatctgt tcggcgcgca tatagttata tttgcgctgc
    11041 gacacgccgt ctgccgccat accgtcatgc agaatggtcg ggcggataaa caccatcagg
    11101 ttacgttttt cttttttatc cgccgtcgat ttaaacaggt taccaatcaa tgggatatcg
    11161 cccagcagag gcactttcgc cacgctttct cccgcctgat cgtccatcag accaccaagc
    11221 acaatcagct caccatcgtt agccagcacg gtggttttca gtttgcgctc accaaacacc
    11281 acgtcgaggc tggtctgtcc ttccaccttc gagacttcct gctcaatcac catctgtacc
    11341 gcgttacctt cgttaatctg cggcgtgact ttcagcatga tgccgacttt tttcctttct
    11401 actgtattga aaggattgct gttattggag ccaacggtag agccggttaa taccggaacg
    11461 tcctagccca ccatgaagaa ggcttcctgg ttgtccagcg tagtgatgct cggcgtggag
    11521 agaacgttcg agctggagtc gtttttaacc gcctgcacca gtgccatcca gtcgcctttc
    11581 accacgccaa ccgccgtacc gctaaatcca gaaagaagct gggcgagtgt ggagagatcg
    11641 ccgttggtat ccggattaat ggtggtagcg ccgttttcac tgatcaccgt cgagcctttc
    11701 tgcggtttcg cctgagaaat ggctgccccc agcgtaccga tagggatctg cgtaccatta
    11761 gcaaactgca ttaatccggc atctttcgac gcccactgca cgccgaagtt gatattgcta
    11821 ccttcggcaa cttccacgat caacgcctcg acgtgcacct gagcacggcg aatatccagt
    11881 tgttcaatta cgctttgcag cgactgcatg atgtcctgcg gcgcggttac aatcagggca
    11941 ttactgtgtt tgctggcggc gatggataca acctcacgcc cgctaccgac cgtgccttcc
    12001 gcctcttctt tagccgccgt gagcgtgccg ctgacctgtt tcagcacatc gaccagatct
    12061 tcggctttgc tgtatttgag atagaaaacc tggctgttgc cgctgcgttc catttctgag
    12121 tccagccgac ggatcaggcg gcgcattttg tcccgcgtgg ccgggtcacc actgacaatc
    12181 acactgttgg tgcgttcgtc ggcgacaatt tgagatttca gcgtcgcagg ctggttctcg
    12241 ccgctgtttt tcgtcaggct ttccagcacg cgggcgattt ccgaagccga ggcgttatcc
    12301 agcgggatca cctcttcggt gcgattaccc gcgtgatcca cgcgctggat cacttccgtc
    12361 aggcgctcca caacggaggc gcgtccggtg agcataatca cgttggaggg atcgtaatta
    12421 acaacgttgc ctgagcctgc gctgtcgatc atctggcgca gaatcggtgc cagctcgcgt
    12481 accgacacat tacgtaccgg cactactttg gtgaccattt catcgcccgc gtagttgtcg
    12541 ctgccttcac cgaccagcgg cagcggctcg actttcgcag cgctggattt caccaccttc
    12601 agcacgtcgt tttccatcgg tacgacggca tacccctgtg cttccagcag gttaaggaat
    12661 agctggtaat actggcgttc attgagcggg gtcatggtgc gaatactcac tttcccctgt
    12721 acgcccggcc ccataatgat ggttttatta aggttagcgc cgacggtttc gatgaacgat
    12781 ttcaggtcgg tatctttaaa attagcggtg aaagtggctt cttccgccca gaccggtgaa
    12841 ctgcataatg ccgctgccag caccagcggc agtaaacgct tttttgtttt gaggccagtt
    12901 gtcttcttac gccagaccga caacgtaata tcacgccaaa acacgatgaa tgattctcct
    12961 agattaaatg cggttagcgc agcgcgatgg aaatgtcgtg gcgcgcaccc ttgcgtaaaa
    13021 ccgtaagttg aatggaatcc attgaaggta actgccgcat cagagcaatc attgctcgtg
    13081 gatcagtgaa atcctgctga tttagcgcaa tggcgatatc gccttccttg aaaccgctgg
    13141 catcgaacag agaacgatct gcccccggct tcactgcata accgacaatt ccctccttac
    13201 gcacaggcgt aagctggata tagttaaaaa ttttctgcgg atctttcgcc agtgcctgac
    13261 gcacggcagt cggtatctca actggcgcac tgacagcagg ttcagcaaca gcttgctttg
    13321 cttcgtcact gacagctttt ttgttggtcg cggcaacggt ggaacgctcc tcttcagcca
    13381 ggctcaggcg ctctattttt ccctgatagc gcagcatcac atggtcgcga ttgatttcct
    13441 caatcaccgc gttgtgcgag ccaagcgttt caccctgcaa atagacctgc tgtttaccgc
    13501 cttcttcaat aaccgcgccg ggtctggcac caaaggcgat cccgcgcgca ccacattaag
    13561 acgcgtttct gccacaggca caggttcggg ttgttttacc ggcgtggcga cgggctgata
    13621 tttgccaaac cagttttgct ggctgattaa ttgcacgtca tttttatcga acgtttttgc
    13681 atccgcacgg ggcggtttat tcgccgatgg ggaaacagcc gtatattccg cagaaaagga
    13741 gaaatagcgc cagagtgaat gcgccacttt tgcagaaata ataagcagca tcagccagaa
    13801 catcccgcgt acaatttt
//
LOCUS       DADLFA010000105        13671 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000105 DADLFA010000000
VERSION     DADLFA010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13671
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.105"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..1126)
                     /gene="hemX"
                     /locus_tag="JQH65_002623"
     CDS             complement(<1..1126)
                     /gene="hemX"
                     /locus_tag="JQH65_002623"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HAY6139995.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEA"
     gene            complement(1148..1888)
                     /gene="hemD"
                     /locus_tag="JQH65_002624"
     CDS             complement(1148..1888)
                     /gene="hemD"
                     /locus_tag="JQH65_002624"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HAY6139996.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLRCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(1885..2826)
                     /gene="hemC"
                     /locus_tag="JQH65_002625"
     CDS             complement(1885..2826)
                     /gene="hemC"
                     /locus_tag="JQH65_002625"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HAY6139997.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDARTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            3213..5759
                     /gene="cyaA"
                     /locus_tag="JQH65_002626"
     CDS             3213..5759
                     /gene="cyaA"
                     /locus_tag="JQH65_002626"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HAY6139998.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(5799..6119)
                     /gene="cyaY"
                     /locus_tag="JQH65_002627"
     CDS             complement(5799..6119)
                     /gene="cyaY"
                     /locus_tag="JQH65_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HAY6139999.1"
                     /translation="MNDSEFHRLADQLWLTIEEHLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            6582..6776
                     /locus_tag="JQH65_002628"
     CDS             6582..6776
                     /locus_tag="JQH65_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004987677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY6140000.1"
                     /translation="MKNVFKVLTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGMVHPR"
     gene            6821..7645
                     /gene="dapF"
                     /locus_tag="JQH65_002629"
     CDS             6821..7645
                     /gene="dapF"
                     /locus_tag="JQH65_002629"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAY6140001.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            7642..8349
                     /locus_tag="JQH65_002630"
     CDS             7642..8349
                     /locus_tag="JQH65_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HAY6140002.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            8346..9242
                     /gene="xerC"
                     /locus_tag="JQH65_002631"
     CDS             8346..9242
                     /gene="xerC"
                     /locus_tag="JQH65_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAY6140003.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            9242..9958
                     /gene="yigB"
                     /locus_tag="JQH65_002632"
     CDS             9242..9958
                     /gene="yigB"
                     /locus_tag="JQH65_002632"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HAY6140004.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            10042..12204
                     /gene="uvrD"
                     /locus_tag="JQH65_002633"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             10042..12204
                     /gene="uvrD"
                     /locus_tag="JQH65_002633"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HAY6140005.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCLPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(12249..13151)
                     /locus_tag="JQH65_002634"
     CDS             complement(12249..13151)
                     /locus_tag="JQH65_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HAY6140006.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKSGQYFVSPDNGTLTLVAQTLGIDSVREIDEKANRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAEIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(13234..13665)
                     /locus_tag="JQH65_002635"
     CDS             complement(13234..13665)
                     /locus_tag="JQH65_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6140007.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVGIIRLDAFKTSKEIQFA
                     VQSGPMLLENGVINPRIHPNVASRKIRNGVGINKQGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
BASE COUNT         3118 a         3585 c         3697 g         3271 t
ORIGIN      
        1 tagcttccgt tgtccctgct gccggttgtg ccagcaggtt acgcacgcga gtctgcatca
       61 gtttttccag catcgcctgg ctttgcaggg tttccggaag atccatcgag atattttgct
      121 ggcttaactg gtccacctcg tcgaggaacg ctttggtggt ggcatcatca gtatcgtagt
      181 aagcacgtac ccaggtggag acgttctcca gcgcctggcg ataagtctct tcctggtgac
      241 gcggtacagc ttgtgctgcg accagcaggc gagagcgaat attttcgcgc agatagatat
      301 cctgatttgg cgctaacagc ggtacggcgg tgtcatcacg acggcgaatc gtaatgaagt
      361 tgtccataaa gttctgccag cttttttgca gattgatacg ccattcgctg atggaactgg
      421 aaagctcttc accatcggaa tccatcggcg aaccatcgct gtcattatcg gccagacgca
      481 ggttatctac ctgatttgaa agctgattaa gcttaaggat gatgccgtca taatccacct
      541 gcgaaactgc agaaaggctg gcgatatcat cggtaattgc ccgacgaacg gtaatcagac
      601 tcgggtcatt catatccgcc aggctggcat ctgcactttt cagcaacgct gcagcggtcg
      661 tgacgtcctg atcgctccac agcttccgtc cggcgagttt caccagaaaa tcggcctgag
      721 ccagcagcca ggttttagca tcgctgccgg aaatggtggc gaccttttgt tggacttcat
      781 ccaactgttt tgccagcgtt tcttgctgac gattcgcctg cttaagctgt gcagcttgtt
      841 gcttaataat gccttccagc tcggcttttt ggctctcctg ggctttttgc aatgccgtca
      901 gttggttagc cagggcatcg ctggtggcgg tctgattgac ggcctgttgt ttaccccagc
      961 catacaaacc aacgcccgcc gccagagcaa tagcgatagc caccgcgctg agaatcaatg
     1021 cggtattgtt cttactcttt ttttctgttg cgacaggttg tgacgtggtg tccacggcct
     1081 ccctggtctc ttcaaccacg gcggaggttt tttcttgttc cgtcattatg gcttcctgtt
     1141 atgagagtta ttgtaatgcc cgtaaaagcg catcgttgtc agcgttatcg gcgaccttaa
     1201 tgtcttgcca gcccagttcc cgggcgagtt tcgccaaacg ctcactgacg accaatagtc
     1261 gacagcgtag taaccagtgc tcacgatacc attgtgggat cagcgaccag agttgctgca
     1321 acatttcacc gctggtaaca acgaccatcg tcacctcgcg ggattgccag cgcatcgctt
     1381 cttctgcacc atcgtaatgg attgcgcatc gttgataaca ttcacaaaaa gtgacctcag
     1441 caccgcgcgc tgtcagggta tccccaatta gctcacgacc gccattgcca cgtaatatca
     1501 gcgcacgttt gcccgcaata ttttgtaatt caggtaattg tagcaagact tcgctgattt
     1561 cccgatcctg cgggtagaga atcttctgtc cgcttacggt atgtagcgcc agtgcggtgg
     1621 tgcgtccaat ggcgaaataa tcaggtagtc ggggccattt acgatcttgc tgatgcagct
     1681 gtgattgggc aaaagcaacc gcgtgttgcg agagggcaaa caacagatcg ctctccccca
     1741 gcgccgccag ttgatcagca agttgcggta attgtcgacc cggagaaaac tcaatcagcg
     1801 gaaaatgcca ggccacctgc cccagtgtgc gcagacggct cactaactct tctccagcgg
     1861 gagacgggcg ggtgaccagg atactcatgc cggggcgtct ccgttataga cttcagcgag
     1921 gatctcgcgc gcgccgttat tcagtagctc ttctgccagc gaaatcccca tttgttcggc
     1981 atcttgcggc gcaccgcggc gttcaccgcg aataatctgc gaaccgtccg gcgcgccgac
     2041 cagcgcacgc agccagattt caccatcaat aagctcggcg tagctaccaa ttggcacctg
     2101 acatccgcct tcgagacggg tattcatggc gcgttctgcg gtaacgcgca gtgcagtttc
     2161 gtggtgattc agcgcggcaa gcagctcgcg agtgcgtgca tcatcaaggc ggcattcaat
     2221 accgaccgca ccttgtccta ccgctggaag agaaatctcg ggtggtaacg cggcgcgaat
     2281 gcgggactcc agacctaaac gttttagtcc ggctacggca agaatgatgg catcgtattc
     2341 gccgttatct agtttgctca ggcgagtgcc gacgttgccg cgcagggagc ggataatcag
     2401 atccgggcgg cgttcagcca gttggcactg gcgacgtaaa ctggacgtcc cgacgatact
     2461 gcctgctggt aacgcatcca gactgtcata gttattggac acaaaggcat cgcgaggatc
     2521 ttcacgctca caaatagtga ccagtcccag accttgcggg aattcaaccg gcacatcttt
     2581 cattgaatgt acggcgatat cggcgcgatt ttcgaggagc gcgacttcca gctcttttac
     2641 aaataagcct tttccgccta ctttcgccag cggcgtatca agaatcacat cgccgcgcgt
     2701 caccatcggt accagttcaa cgaccaggcc cggatggctc gccatcaact tgtctttgac
     2761 atagtgtgcc tgccagagtg caagtgggct ttggcgtgtg gcaattctta aaacattgtc
     2821 taacatgctt gttaccgtca ttatcatccg tggtccatcc taacatcctt gccagagtga
     2881 tgtcagtgtt gtggtgaaac gtagacgcct gcgcaaaccg taaaatgagg tctggcagtg
     2941 gatcctgaca ggcgtttcac gccgtcgtaa taaggaattt acagagaata aacggtgcta
     3001 cacttgtatg tagcgcatct ttctttacgg tcaatcagca aggtgttaaa ttgatcacgt
     3061 tttagaccat tttttcgtcg tgaaactaaa aaaaccaggc gcgaaaagtg gtaacggtta
     3121 cctttgacat acgaaatatc ccgaatgccg cgtgttaccg ttgatgttgg cggaatcaca
     3181 gtcatgacgg gtagcaaatc aggcgatacg tcttgtacct ctatattgag actctgaaac
     3241 agagactgga tgccataaat caattgcgtg tggatcgcgc gcttgctgct atggggcctg
     3301 cattccaaca ggtctacagt ctactgccga cattgttgca ctatcaccat ccgctaatgc
     3361 cgggttacct tgatggtaac gttcccaaag gcatttgcct ttacacgcct gatgaaactc
     3421 aacgccacta cctgaacgag cttgaactgt atcgtggaat gtcagtacag gatccaccga
     3481 aaggtgagct tccaattact ggtgtataca ccatgggcag cacctcgtcc gtagggcaaa
     3541 gttgttcctc tgacctggat atctgggtct gtcatcaatc ctggctcgat agcgaagagc
     3601 gccagttact acaacgtaaa tgtagtctgc tggaaagctg ggccgcctcg ctgggtgtgg
     3661 aagtcagctt cttcctgatt gatgaaaacc gcttccgtca taatgaaagc ggcagcttgg
     3721 ggggcgaaga ttgtggctcc acccagcata tactgctgct tgacgaattt tatcgtaccg
     3781 ccgtgcgtct cgccggtaag cgtattctgt ggaatatggt gccgtgcgac gaagaagagc
     3841 attacgacga ctatgtgatg acgctttacg cgcagggcgt gctgacgcca aatgaatggc
     3901 tggatctcgg tggcttaagc tcgctttctg ctgaagagta ctttggtgcc agcctttggc
     3961 agctctacaa gagtatcgat tccccataca aagcggtact gaaaacactg ctgctggaag
     4021 cctattcctg ggaatacccg aacccacgtc tgctggcgaa agatatcaaa cagcgtttgc
     4081 acgacggtga gattgtatcg tttggtctcg atccatactg catgatgctg gagcgtgtta
     4141 ctgaatacct gacggcgatt gaagatttta cccgtctgga tttagtacgt cgctgcttct
     4201 atttaaaagt gtgcgaaaag ctcagccgtg aacgcgcctg cgtaggctgg cgtcgcgcag
     4261 tgttgagcca gttagtgagc gagtggggtt gggacgaagc tcgtctggca atgctcgata
     4321 accgcgctaa ctggaagatt gatcaggtgc gtgaggcgca caacgagttg ctcgacgcga
     4381 tgatgcagag ctaccgtaat ctgatccgct ttgcgcgtcg caataacctt agcgtctccg
     4441 ccagtccgca ggatatcggc gtgctgacgc gtaagctgta tgccgcgttt gaagcattac
     4501 caggtaaagt gacgctggta aacccgcaga tttcacccga tctctcggaa ccgaatctga
     4561 cctttattta tgtgccgccg ggccgtgcta accgttcagg ttggtatctg tataaccgcg
     4621 cgccaaatat tgagtcgatc atcagccatc agccgctgga atataaccgt tacctgaaca
     4681 aactggtggc gtgggcatgg tttaacggcc tgctgacctc gcgcacccgt ttgtatatta
     4741 aaggtaacgg cattgtcgat ttgcctaagt tgcaggagat ggtcgccgac gtgtcgcacc
     4801 atttcccgct gcgcttacct gcaccgacac cgaaggcgct ctacagcccg tgtgagatcc
     4861 gccatctggc gattatcgtt aacctggaat atgacccgac agcggcgttc cgcaatcagg
     4921 tggtgcattt tgatttccgt aagctggatg tcttcagctt tggcgagaat caaaattgcc
     4981 tggtaggtag cgttgacctg ctgtaccgca actcgtggaa cgaagtgcgt acgctgcact
     5041 tcaacggcga gcaatcgatg atcgaagccc tgaaaactat tctcggcaaa atgcatcagg
     5101 acgccgcacc gccagatagc gtggaagtct tctgttatag ccagcatctg cgcgggttaa
     5161 tccgtactcg cgtgcagcaa ctggtttctg agtgtattga actgcgcctt tccagcaccc
     5221 gccaggaaac cgggcgtttc aaggcgctgc gcgtttctgg tcaaacctgg ggcttgttct
     5281 tcgaacgcct gaatgtatcg gtacagaagc tggagaacgc catcgagttt tatggcgcta
     5341 tttcgcataa caaactgcac ggcctgtcag tgcaggttga aaccaatcac gtcaaattac
     5401 cggcggtggt ggacggcttt gctagtgaag ggatcatcca gttcttcttc gaagaaacgc
     5461 aagacgagaa tggctttaat atctacattc ttgacgaaag caaccgggtt gaggtgtatc
     5521 accactgcga aggcagcaaa gaggaactgg tgcgtgacgt cagtcgcttc tactcgtcat
     5581 cgcacgaccg cttcacctac ggctcaagct tcatcaactt caacctgccg cagttctatc
     5641 agattgtgaa ggttgatggt cgtgaacagg tgattccgtt ccgcacaaaa tctatcggta
     5701 acatgccgcc tgccaatcag gatcacgata cgccgctatt acagcaatat ttttcgtgat
     5761 gaacgtgccg gaaagcgagg cttatccggc atgcaatctt agcggaaact gactgtttca
     5821 cccgcctgct gcgtcgccgc ctgttccagc aaatcccaga aggtttcgcc gctgcgatcg
     5881 caaatccact catcgccttt cagatcaaaa tggtagccgc cttgtttggt tgccagccat
     5941 acctggtgca gcggttcctg gcggttgata atgattttgc tgccgttttc aaaggtaatg
     6001 gtcagcacgc cgccgttgat ttcgcagtcg atatcgctgt cgccatccca gtcgtccaga
     6061 tgctcttcaa tggtcagcca cagttgatca gccaggcgat gaaattcact gtcgttcatt
     6121 gttgtatcct gtttttaagt gatggcggca gtatagcggc atggggtcag ggcttcaaag
     6181 tttgcacctc tgctgctgcg ttccggcatg attcatccgt caccggaata atgatgtctc
     6241 tgtgtggcga aagatttgtc tcttcattag ggcgcagtta caccacgcct ttccctgttt
     6301 ctggtaaaca ttatgatcag gtttaccgag cgagcatcct cacgctgacg gaactaaaaa
     6361 agccaacaaa caaaccacat tgcgatagtg cataaagcca tcctggcgcg aggtgccgat
     6421 cacgaaactg ccagcagaac ataaatcctc acgagtaagc gttatactcg cagcatttcc
     6481 tcacttttca gacttcataa agagtcgcta aacgcttgct tttacgcctt ctcctgcgat
     6541 gatagaaagc agaaagcgat gaactttaca ggcaatccat aatgaaaaac gtgtttaagg
     6601 tactcactgt attacttact ctcttcagcc tgacgggctg cggtctgaaa ggtccgctct
     6661 atttcccgcc tgcagataaa aatgcaccgc cgccgaccaa accggtagag acgcaaacgc
     6721 aatccacggt gccggataaa aacgatcgcg ccactgggat ggtccatccc aggtgaatta
     6781 ctaaaagtca gtttctgtac ccgcgtgatt ggagtaaatg atgcagttct cgaaaatgca
     6841 tggccttggc aacgatttta tggtcgtcga cgcggtaacg cagaatgtct ttttttcacc
     6901 ggagctgatt cgtcggctgg cggatcggca cctgggggta gggtttgacc agctgctggt
     6961 ggttgagccg ccgtatgatc ctgaactgga ttttcactat cgcattttca atgctgatgg
     7021 cagtgaagtg gcgcagtgcg gcaacggtgc gcgctgcttt gcccgttttg tgcgcctgaa
     7081 agggctgacc aataagcgcg atatccgcgt cagcaccgcc aacgggcgga tggttctgac
     7141 cgtcaccgat gatgatctgg tccgcgtaaa tatgggcgaa cccaacttcg aaccttccgc
     7201 tgtgccgttt cgcgctaaca aagcggaaaa gacctatatt atgcgcgccg ccgagcagac
     7261 aatcttatgc ggcgtggtgt cgatgggaaa tccgcattgc gtgattcagg tcgatgatgt
     7321 cgataccgcg gcggtagaaa cgcttggtcc tgttctggaa agccacgagc gttttccgga
     7381 gcgcgccaat atcggtttta tgcaagtggt taagcgcgag catattcgtt tacgcgttta
     7441 tgagcgtggg gcaggagaaa cccaggcctg cggcagcggc gcgtgtgcgg cggttgcagt
     7501 agggattcag caaggtttgc tggccgaaga agtacgcgtg gaactccccg gcggtcgtct
     7561 tgatatcgcc tggaaaggtc cgggtcaccc gttatatatg actggcccgg cggtacatgt
     7621 ctacgacgga tttattcatc tatgaagcaa ccaggggaag aactgcagga aacactcacg
     7681 gagcttgatg accgggcggt tgtcgattat ctgattaaaa atcctgagtt ttttatccgt
     7741 aatgcgcgcg cagtagaagc gatacgtgtg ccacatccgg tacgcggcac cgtttcgttg
     7801 gtcgagtggc acatggcccg cgcacgtaat catattcatg ttctggaaga gaacatggcg
     7861 ctgttgatgg aacaggctat cgccaacgaa ggcctgtttt atcgcctact ctacctgcag
     7921 cgcagtctca ccgccgccag cagtctcgac gatatgctga tgcgctttca ccgctgggcg
     7981 cgcgatctcg gcctggcagg tgcgagtctg cgcctgtttc cggatcgctg gcgcttaggt
     8041 gcgccgtcga accacactca tctggcatta agccgtcagt ctttcgaacc gctgcgtatt
     8101 cagcgtttgg ggcaggaaca gcactatctt gggccgctta acggaccaga gctgctggtg
     8161 gtgctaccgg aagcgaaagc ggtgggatcg gtggcgatgt cgatgctggg aagcgatgct
     8221 gatttgggtg tcgtgctgtt taccagtcgc gatgccagtc actatcaaca agggcaagga
     8281 acgcagttac ttcatgaaat tgcgctgatg ttgccggagc ttctggagcg ttggattgaa
     8341 cgcgtatgac cgatttacac accgatgtag aacgctacct acgttatctg agcgtggagc
     8401 gccagcttag cccgataacc ctgcttaact accagcgtca gcttgaggcg atcatcaatt
     8461 ttgccagcga aaacggcctg caaagctggc agcaatgtga tgtgacgatg gtgcgcaatt
     8521 ttgctgtacg cagtcgccgt aaagggctgg gagcagcaag tctggcgtta cggctttctg
     8581 cgctacgtag cttttttgac tggctggtca gccagaacga actcaaagct aacccggcga
     8641 aaggtgtttc ggcaccgaaa gcgccgcgtc atctgccgaa aaatatcgac gtcgacgata
     8701 tgaatcggct gctggatatt gatatcaacg atcccctcgc tgtacgcgac cgtgcaatgc
     8761 tggaagtgat gtacggcgct ggtctgcgtc tttccgagct ggtggggctg gatatcaaac
     8821 acctcgacct ggagtctggt gaagtgtggg taatggggaa aggcagcaaa gagcgccgcc
     8881 tgccgattgg tcgcaacgct gtggcgtgga ttgagcactg gcttgatttg cgcgacctgt
     8941 ttggtagcga agacgacgcg ctttttctgt cgaaactggg caagcgtatc tccgcgcgta
     9001 atgtgcagaa acgctttgcc gaatggggca taaaacaagg gctgaataat cacgttcacc
     9061 cacataaatt acgtcactcg ttcgctacgc atatgctgga gtcgagcggc gatcttcgtg
     9121 gtgtgcagga gctactgggt catgccaacc tctccaccac gcaaatctat actcatcttg
     9181 attttcaaca ccttgcctcg gtgtacgatg cggcgcatcc acgcgccaaa cgggggaaat
     9241 aatgcgtttt taccggcctt tggggcgcat ctcggcgctc acctttgacc tggatgatac
     9301 cctttacgat aaccgtccgg tgattttgcg caccgagcga gaggcgctta cctttgtgca
     9361 aaattatcat ccggcgctgc gcagcttcca gaatgaagat ctgcaacgcc tgcgccaggc
     9421 ggtacgggaa gcggaacccg agatttatca cgacgtgacg cgctggcgtt ttcgttcgat
     9481 tgaacaagcg atgctcgacg ccgggctgag tgccgaagaa gccagtgcag gcgcacacgc
     9541 agcaatgatc aactttgcca aatggcgcag ccgaatcgac gtcccgcagc aaactcacga
     9601 caccttaaaa cagctggcga agaaatggcc gctggtggcg atcaccaacg gtaacgccca
     9661 gccggaactg tttggtttgg gggattattt tgagtttgtg ctgcgcgctg gcccgcacgg
     9721 gcgctcaaaa ccgttcagcg atatgtactt tctggctgcg gaaaaactca acgtgccgat
     9781 cggcgagatc ttacatgttg gggacgatct caccactgac gtgggtgggg caattcgcag
     9841 cggaatgcag gcctgttgga tcagaccgga aaatggcgat ctgatgcaaa cctgggacag
     9901 ccgtttactg ccgcatctgg aaatttcccg gttggcatct ctgacctcgc tgatataatc
     9961 agcaaatctg tatatatacc cagctttttg gcggagggcg ttgcgcttct ccgcccaacc
    10021 tatttttacg cggcggtgcc aatggacgtt tcttacctgc tcgacagcct taatgacaaa
    10081 cagcgcgaag cggtggccgc gccacgcagc aaccttctgg tgctggcggg cgcgggcagt
    10141 ggtaagacgc gcgtactggt gcatcgtatc gcctggctga tgagcgtgga aaactgcttg
    10201 ccatactcga ttatggcggt gacgtttacc aacaaagcgg cggcggagat gcgtcatcgt
    10261 atcgggcagc taatgggcac cagccagggc ggcatgtggg tcggcacctt ccacgggctg
    10321 gcgcaccgtc tgctgcgtgc gcaccatatg gacgccaatc tgccgcagga ttttcagatc
    10381 ctcgacagcg aggaccagct acgcctgctc aaacgcctaa tcaaggcgat gaacctcgac
    10441 gagaagcagt ggccgccgcg ccaggcaatg tggtacatca acagccagaa agatgaaggc
    10501 ctgcgtccac atcatattca aagctacggt aatccggtgg agcagacctg gcagaaggtg
    10561 tatcaggcgt atcaggaagc gtgtgaccgc gcgggcctgg tggacttcgc cgagctgctg
    10621 ctgcgcgctc acgagttgtg gcttaacaag ccgcatatcc tgcaacacta ccgcgaacgt
    10681 tttaccaata tcctggtgga cgaattccag gataccaaca acattcagta cgcgtggatc
    10741 cgcctgctgg cgggcgacac cggcaaagtg atgatcgtcg gtgatgacga ccagtcaatc
    10801 tacggctggc gcggggcgca ggtggagaat attcagcgtt tccttaatga tttccccggt
    10861 gccgaaacta ttcgtctgga gcaaaactac cgctctacca gcaatattct gagcgccgct
    10921 aacgccctga ttgaaaacaa taacgggcgt ctgggtaaaa aactgtggac cgatggcgcg
    10981 gacggtgagc ctatttccct ctattgcgct tttaacgaac tcgatgaagc gcgttttgtg
    11041 gttaaccgca tcaaaacctg gcaggacaac ggcggggcgc ttgccgagtg cgccattctc
    11101 taccgcagta acgcccagtc gcgtgtgctg gaagaagcac tgttgcaggc gagtatgccg
    11161 taccgtattt acggcgggat gcgcttcttc gaacgccagg aaatcaaaga tgcgctctcg
    11221 tatctgcgcc tgattgccaa ccgtaacgac gacgcggcct ttgagcgtgt ggtgaatacg
    11281 ccaacgcggg gtattggtga ccggacgctg gacgtggtac gtcagacatc gcgcgatcgc
    11341 cagttaacac tctggcaggc atgtcgtgaa ctgttgcagg aaaaagccct cgccggacgt
    11401 gctgccagcg ccttacagcg gtttatggaa ctgatcgacg ccttagcgca ggaaactgcc
    11461 gatatgccgc tgcatgtaca gactgaccgg gtaattaaag actccggcct gcgtaccatg
    11521 tacgagcagg agaagggcga aaaaggtcag acgcgtatcg aaaacttaga ggaactggtg
    11581 acggcaacgc gccagttcag ctacaacgaa gaagacgaag atttaatgcc gctgcaggcg
    11641 ttcctatccc atgcggcgct ggaagcaggc gaagggcagg cggatacctg gcaggatgcg
    11701 gtacaactga tgaccatgca ctcggcgaaa ggtctggagt tcccgcaggt gtttatcgtc
    11761 ggtatggaag agggcatgtt cccaagccag atgtcgctgg atgaaggcgg gcgtctggaa
    11821 gaagaacgcc gtctggccta cgttggcgta acccgcgcga tgcagaaact gacgctgacc
    11881 tacgcggaaa ctcgccgtct gtatggcaaa gaggtttacc atcgcccgtc gcgctttatt
    11941 ggcgagctgc cggaagagtg tgtggaagag gtgcgcctgc gcgccacggt aagccgcccg
    12001 gtcagccatc agcggatggg tacgccgatg gtcgagaacg acagcggcta caagctcggc
    12061 cagcgtgtac gccacgctaa gtttggtgaa ggcaccatcg tcaatatgga aggcagcggt
    12121 gaacatagcc gtttgcaggt ggcattccag ggccagggta ttaaatggct ggtggcggct
    12181 tatgcccggt tggagacggt gtgacgttgc gggatgcggt gcgccacacc gcattccgca
    12241 tcagcacctt acttagcgca cttcttcaca tcaatattcc agtcagcacc agacgcaacc
    12301 tgatgtttct gcgcgaaatt atccatattc agcgccacgg aaacattcaa taagctgttc
    12361 agatacacca tcggctggcc ttccggcaca tcgccgaagc ttgcgacata cggcattttc
    12421 cctgcgtatt tttgctggga accttcggaa atggtcacac acaacgtgtc gttgagtttg
    12481 atttctgcct gattcagcaa ctcgtcgctg atattgctcc agacgttgcc gtactgaata
    12541 tcgagaatcg ggatattgcc cttcacttca ccttttgttg cttttgcttt ctgataagaa
    12601 agttcgacaa cttttgctgg aagctctggc cccacctgtt cgaacgtaat cgccccggaa
    12661 gccagacgtg caccggtgta ggcatacacg tcacgaccgt ggaaggtata tgacttttca
    12721 gaacctttca ggcggttagc tttttcgtcg atttcccgga cgctatcaat acccagagtt
    12781 tgtgccacca aagtgagcgt accgttatct ggtgaaacga agtattgacc gcttttggtt
    12841 ttcagcacta ccgatttacg gtcagtgcct acgcccggat cgaccacgct aacaaacacc
    12901 gaaccttgcg gccagtaact ggcagtctga tacaggcgat aagcgccttc ccagatgtta
    12961 tacggtggaa tttcgtgtgt taaatcaaag attttaagat tatggtcaac gccgaatgcg
    13021 acccctttca tcgccgagac ggcaccatct ttcaggctaa aatcagtttg taaaatcaaa
    13081 gcgttatccg cataggcgga tgatgatccc agacacgtta ataaaaataa cgttgcgagc
    13141 gtttttttca tttttgtttc ccttcattta tctgatgatt ttccagctgt gtagccagtc
    13201 ccagtttgtc tggcctcctc gagtgtacaa tgcttaacct ttccgctcga cggaaatcat
    13261 ggtgacaaaa ggatagcgtt gccacgggat tgccccgcct ttcatataca tatgtgaaat
    13321 tgtgccatca agataaagca actgctcaac gttcagtttc gctttggcat aacaggcaaa
    13381 atcataaaaa tttgtcgcct gctggctcaa caaaaacacg gcgttccctt gtttattaat
    13441 ccctacaccg ttacgaattt tacgcgaggc aacattcggg tgaatgcgtg gattaattac
    13501 gccgttttct aataacattg gccctgactg caccgcaaac tgaatctctt tactggtttt
    13561 aaaggcatcc agacgaatga tgccgacttt atctcccgca acataaaaca cgccgccagg
    13621 acggataaag aaattccctt cacctgaagc gagatttaac gccaccttct g
//
LOCUS       DADLFA010000106        13584 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000106 DADLFA010000000
VERSION     DADLFA010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13584
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.106"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(241..1614)
                     /gene="cobA"
                     /locus_tag="JQH65_002636"
     CDS             complement(241..1614)
                     /gene="cobA"
                     /locus_tag="JQH65_002636"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HAY6140008.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFTTMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            complement(1633..2439)
                     /gene="nirC"
                     /locus_tag="JQH65_002637"
     CDS             complement(1633..2439)
                     /gene="nirC"
                     /locus_tag="JQH65_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HAY6140009.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKSNRPVADKFNQTETAAG"
     gene            complement(2566..2892)
                     /gene="nirD"
                     /locus_tag="JQH65_002638"
     CDS             complement(2566..2892)
                     /gene="nirD"
                     /locus_tag="JQH65_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HAY6140010.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            complement(2889..5432)
                     /locus_tag="JQH65_002639"
     CDS             complement(2889..5432)
                     /locus_tag="JQH65_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit"
                     /protein_id="HAY6140011.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRIFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            complement(5694..6875)
                     /gene="tsgA"
                     /locus_tag="JQH65_002640"
     CDS             complement(5694..6875)
                     /gene="tsgA"
                     /locus_tag="JQH65_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HAY6140012.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            7146..7718
                     /gene="ppiA"
                     /locus_tag="JQH65_002641"
                     /gene_synonym="rotA"
     CDS             7146..7718
                     /gene="ppiA"
                     /locus_tag="JQH65_002641"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HAY6140013.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGAPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            7823..7990
                     /locus_tag="JQH65_002642"
     CDS             7823..7990
                     /locus_tag="JQH65_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HAY6140014.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLVELRRHYER"
     gene            7980..8582
                     /locus_tag="JQH65_002643"
     CDS             7980..8582
                     /locus_tag="JQH65_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HAY6140015.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKTKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            8614..9177
                     /gene="pabA"
                     /locus_tag="JQH65_002644"
     CDS             8614..9177
                     /gene="pabA"
                     /locus_tag="JQH65_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HAY6140016.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPGSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            9263..10483
                     /gene="argD/dapC"
                     /locus_tag="JQH65_002645"
     CDS             9263..10483
                     /gene="argD/dapC"
                     /locus_tag="JQH65_002645"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HAY6140017.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTASTPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(10550..12640)
                     /locus_tag="JQH65_002646"
     CDS             complement(10550..12640)
                     /locus_tag="JQH65_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6140018.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(12691..13323)
                     /gene="crp"
                     /locus_tag="JQH65_002647"
     CDS             complement(12691..13323)
                     /gene="crp"
                     /locus_tag="JQH65_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HAY6140019.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
BASE COUNT         3289 a         3495 c         3556 g         3244 t
ORIGIN      
        1 tccctatgta ttatatatta attaatattc attgattata tgaatataag cagactgctc
       61 gttaagaacg atacaggaat atttaattgg cgtcagtgag cgaatgactt aattttgtaa
      121 gtcgctgtaa cggtgaatat aacccattag cagataatat ttattaaggg gttatgtttt
      181 atttgctgaa tatataaaaa gagtcattaa gaaggccaga attctctggc ccgggttaaa
      241 ttaatggttg gagaaccagt tcagtttatc gcgcaggcca acaacccgac caataataat
      301 tagcgatgga ctgttcattt gctgagcaag ttcaccgagt tgcgtgagcg taccgtcaat
      361 cacgcgctgc gtgactgccg ttccgttttc gacaattgcc accggcattt cgccaggcat
      421 accgtgttca atcagctttt gctgaatagt cgcggcctga ttcaacccca tatagaacac
      481 cagcgtctgt ttttctgccg ccaggttttc ccagtccagc tcgccaccgg tttttaagtg
      541 tccggtaatt aagcgtacgc tctgggcata atcgcgatgc gtgagtggaa tacccgaata
      601 ggcagagcaa ccagaagctg cggtaatacc cggaaccacc gagaacggaa tacccgcgtt
      661 gcacagtgtt tccagctctt cgccaccacg gccaaaaata aacggatcgc cacctttcag
      721 ccgcaccacg cgtttgcctt tttgcgcttc ccgcagcagg atctggttaa tctcttcctg
      781 cggtacacag tggtatcccg cgcgtttgcc gacgaaaacg cgatcagcat cgcggcgtac
      841 cagattcata atatcgtcag aaaccagacg gtcgtagacc accacatctg cctgctgaat
      901 ttgttgcagt cctttcagcg tcagcagccc ggcatcgccc ggacctgcac caaccagcac
      961 cacttcaccg cgatggtcga gcggttcgtt gattaactgt tcggtcgttt cagtaatggc
     1021 tttctgatcg ttgtttgcca gcgactgcgc caggcggtcg ttaacgaaca atttctccca
     1081 gaaacggcga cgctcactca tcgtggtgaa ctgttgtttc actcgcccgc gtaattgccc
     1141 ggcatatttc gccacctggc ccagatgcaa cggcagcagt gattcgagtt tttcgcgcaa
     1201 caggcgcgcc agaaccggag aggtgccgcc agaggagacc gctaccatta gcggtgagcg
     1261 gtcaataatc gacggcataa taaagctggc ggctttcggc gcatcgacta cgttacagaa
     1321 gatgcgacga gcttcagcgg cttcgctgac gcgctggtta agcgtgtcat catccgtcgc
     1381 tgcaatcgcc agccagcagg tgtcgagaag gctttcatca aatggccctt cgacgagggt
     1441 taacatgcct gcatctgccc atgcggtgaa ctgtggaata aacgctaatg cattcaccgt
     1501 taagcgagcg cctgcgtcta acagcaacct tgctttgcgt tccgcgacat caccaccgcc
     1561 gacaatcaga cagtcgcgat cgcgtaattg gcaaaatata ggcaaatgat ccacgtaaaa
     1621 accccttagt aattaaccgg cagccgtttc agtttgatta aatttgtccg caaccggacg
     1681 attcgatttc ggcgtagcat accaataacc caatcccatg aatacggcac ctgataaagt
     1741 attacccagc gtcacccaca gcaggttatg accaataccc gccagcgtgt aggcttcgct
     1801 gtggttgccg aaccaggaga gcgcgaacag cgtcatgtta gcgatagagt gctcgtagcc
     1861 ggacgcgata aatgccagca gacaccacca gatagcgata aatttcgccg ccccttcagt
     1921 gcgcagcgcc atccagattg ccaggcaaac cagccagtta cacaatgcac ctttgaagaa
     1981 gagtaccatt gccggtgcag tggttttagc cagcgcgacg gagtgaacga tgctggtatc
     2041 taccggcagc aggctaccgc cgccccagct atagagcatg gcaacgaaga cggaaccgac
     2101 caggttaccc agccaggttt gcggcaggat tgcccacatt tgcccgtggc tgatgctgcc
     2161 cgcttttacc ccaaaggtga ggaacatggt gtgtccggtg aacagttcag aaccggcgat
     2221 aatcaccagc gttaaggcga taccaaaggt cgcgcccatc accagcggac gtacggacgg
     2281 gtcgagcaaa ttaccgagcg tgaaaatcaa gatgatccca agacccacat acgcgcccgc
     2341 catggcggag ctgacccaaa agccgagcgg gttatttgcc gacaggcgtg caatgcgcgc
     2401 agcgttagcc gcacacttat taatagtgtc tgtaaacatt tgattatcct ttaaaaaata
     2461 aaaacaaaaa aataaaacaa aaagtttaaa aaatgtcctg caatataccg aaggtaatta
     2521 caggaccatg caaaaagggg aggcattgcg cctcccgtta aaacattaac cgcgcagctg
     2581 caccacgccg tctttcactc gtgcttcgta atgtttgacg gaaaactgtt cgtcttccat
     2641 gcacaagcca tcacttaagc gaaaacgctg ttttttcagc gggctggcga cccacagctc
     2701 gccctggtgt tccgcaatca gcccgcgtga cagcacgctg gactcgaaga acggatcgat
     2761 gttgctgatc gcaaacacct ggtcgctgtg atacgggcgg aaaatcgcga cctgctcgtc
     2821 gcctaacagc gcgcagacgc cggtttcagg caggatgtca tcgattttgc agatgtcttt
     2881 ccactggctc atgcgttgtc ctccaccaga gttaccggga tacgttcata cggcgttgcc
     2941 ggacggtgct gttcgcgctc tggcaccatc tgcacgttcg ggtcacgctt gtcgctgttg
     3001 atgaagtgtt tgaagcgagt ctgcgcggac ggtgtattga ccgtttcagt ccactcacac
     3061 actaccgctt cacgcaggcg cgccatctct tcttccagat gagcgttcag ccccagtttg
     3121 tcgtcaatga tcaccgcttt caggtaatcg atgccgcctt cgaggttttc taaccacggc
     3181 gcggtacgcg tcagtttgtc ggcggtacgg atgtagaaca tcatgaagcg gtcgagatat
     3241 ttgatcagcg tttcgcgatc gatatcagct gccagcagat ccgcatgacg cggtttcatg
     3301 ccgccgttgc cgcaaacata caggttccag cctttttcag tggcgataat acccacgtct
     3361 ttaccctgag cttctgaaca ttcacgggta cagccggaga caccgaactt cattttgtgc
     3421 ggcgtacgga tgcctttgta gcggttttcc agttccacgc cgaggccgac gctgtcgcca
     3481 acgccgtagc ggcaccaggt gctacccacg caggttttcg ccatacgcag tgctttcgca
     3541 taggcatgac cggtttcgaa gccggcttca atcagctgac gccagatctc tggcagatcg
     3601 tctttctgtg cgccaaacat cgccaggcgc tgggaaccgg tgattttggt gtagagatta
     3661 aattcacgcg cgatacgacc cactgccatc agcccttccg gggtgatttc accgcccgga
     3721 gaacgcggga tcaccgagta ggtcccgtct ttctggatgt tagcgaggaa gttgtcgtta
     3781 gaatcttgca gcggagtatg ttctggcttc agaatgtatt cgttccagca ggaggccagc
     3841 agcgaaccga cggtcggttt acaaacttca caaccgtagc ctttgccgtg tttcgccagc
     3901 agttcttcga aggttttaat gccttcaacg cggatcaaat ggaacagttc ctgacgcgaa
     3961 taagcaaagt gttcgcacag gttgttgtta acttcgatgc cctgtttcgc cagttccgcg
     4021 ttcagtacct gagtgaccag cgggatacag ccaccgcagc cagtacccgc tttggtttca
     4081 gctttcagcg ccgcaactgt gtggcagcct ttgttgatgg cagcaatcag atcaccttta
     4141 gtgacgtcga agcaggagca gatttgcgcg ctgtccggca gtttatcaac accgatagac
     4201 ggcttaccgc tacccgagtg cgccggcaga atcagagaat ccgggttttc cggcagctcg
     4261 atagcattca gcaccagttg cagcaagtta ccgtagtcgc tggtatcgcc caccagtacc
     4321 gcaccgagca gggttttgtt gtcttcgctg acgatcaggc gtttgtagat ctctttgctt
     4381 tcgtcgaggt aaacgtagct acgtgcgcca ggcgtgcgac catgagcatc accaataccg
     4441 cctacgtcca cgcccagcag tttcagcttg gcgctaaggt cagcaccttc aaaggcgttt
     4501 tcgctaccga gaatatggtc aacggcgacc tgcgccattt tgtagccagg tgctaccaga
     4561 ccaaatatac ggttgttcca gcttgcgcat tcaccgatag cgtagatatc cggatcggaa
     4621 gtttggcagg aatcattaat gacgataccc ccacgcggag caacgtccag accacactgg
     4681 gttgccagct tatcgcgcgg acggataccg gtagagaaga cgataaagtc gacttccagt
     4741 tcgctgccgt cggcaaaacg catggtttta cgtgcttcga caccttcctg cacaatctca
     4801 agggtgtttt tgctggtgtg aacgcgcacg cccatacttt cgattttgcg acgcagctgc
     4861 tcgccaccca tctgatcaag ctgttccgcc atcagcatag gggcaaattc gataacgtgg
     4921 gtttcaatac ctaagttttt cagcgcgcca gcggcttcca gacctaacag gccgccacca
     4981 acaacggcac cgcgtttgct gcgacgggcg caggattcaa tggcgttgag gtcttcaatg
     5041 gtgcgataga caaagcagtc ctgagtatca gaacctttga ttggtgggat ccacgggtag
     5101 gaaccggttg ccatgatcag cttgtcataa aaaacggtac gtccggcgct ggagtgaatc
     5161 accttctcct gacggttgat ggtgatagcg cgttcgccga ccagaacttt gatgccgtgt
     5221 ttctcgtaga agccttcgcg caccagcgac agctcttcgg cggtgtggtg agagaagtaa
     5281 gacgagaggt gtacgcggtc ataagcgatg cgcggttctt cacagaaaac ggtaatatca
     5341 aagttggccg catcagattt atcaagaaga tcttcgataa agcgatggcc gaccataccg
     5401 ttaccgataa ttgcgagtct gactttgctc atttttgcct cgatttcttt tctattaccg
     5461 cctaccttaa cgattcagca accccgctta ttgatgtaaa tcaaattcac ctttatatac
     5521 cccttaaggg gtatattgct gatttgtata aattttctta agtcacggaa ttgtctgaca
     5581 tttcatgttt gtgcaaaaaa tagacaaaat gaacgggttt atagaaatag atgcacgtag
     5641 cggggttaga gaggaaaagg ccggacgacg atagtgccgt ccggcattag caattaatga
     5701 gaggtcaggg tgttatgctg acggtgacgg ctgacgaaac ctaacaggaa gcacatcaca
     5761 aagacgacag cgtacagacc gtttgctgta agcagtgccg cctgcggacc gctatgttca
     5821 acaatcgggc cggtaaccac aaaggtcaac atagtaccga tggttccgca ggtcaggaca
     5881 aagttaacca gttttggcga cggtactttg gtctgctgtg aacccagggt gatgatggtg
     5941 gtatagatcg cgctggagaa gaagcccaga gcgagaattg accacgccat atgtgctggt
     6001 gttccggtgt taaagacgta catcagaatc gcagccagac cagccagtac ggtcagaatg
     6061 cgttgcaaat caaagaagcg aagaataaag ctgaacgccc acatgccgac catgtatgac
     6121 atccagaagt tactcaccag cgtgcccgcg tcgttcaggc tcatgcccag gcctttggca
     6181 tactcaggca cccaggagat aaaacctaac tgaccgagga tgtagcacag cgccgcaaca
     6241 gagagaaaca gtacgccgat cccccacttt tctttctcta ccggagcatc cgtttttggt
     6301 gcatgtttgc ccagtgctgg gaactcacag ccgaaggtca gaataaaaat agcgacatac
     6361 accagcccga tgcaggcata aacccagtac cactcaatgc tgcgcgccag tagaaacgcg
     6421 gcgatcattg ggaaaatcat cccagccata ctgaagaagg agtcagtaaa taacaggcgg
     6481 gaaccgcgct gacgcccttc atacatttgt gttatcagga atgtaccaat cgacatagta
     6541 atgccgctga ccaccccgag aatgaacatc gccgtcgaga acagcgccag gctgtggctg
     6601 aacatcaaac cggcaaccgc cagcaccatc aggagaaagc caaaacgtaa ctgcgttttc
     6661 aacgggacga tttccatcag ccaggcgttg aggaagatag agattaaaat gccggcgttg
     6721 aggaaggtga aggtgttact catactggaa acaggcagat tgaaataatc ggcgatattt
     6781 cccatcacca tcccggtgac aataaccaac gcaccagtca gtgcgtagga gagaaagcta
     6841 atccatgtga gcttgatgcg attgctgtta gtcatgattg gcctgcgttc aaaaataaaa
     6901 tggcatagcg ggatatgccg cgagcgggtg attttaggtg attttgtgat ctgtttaaat
     6961 gttttattgc aatcggttgc taaattgcat tttaagaagt gatcttgatc acggaataaa
     7021 aagtgatcgt caggttacat atatttcaga tacgtaaaat taggtaaagg gatggccttg
     7081 ttcttgaagg ctatttagaa tctcttcact tgcttttttt ctgctctgtt tgttaaggaa
     7141 atctcatgtt caaatcgacc ctggcggcga tggctgctgt tttcgctctt tctgctcttt
     7201 ctcccgcagc aatggcagcg aaaggggccc cgcacgtatt gttgacaacc tcagctggta
     7261 acatcgaact ggagctggat aaacaaaaag cgccagtgtc tgtgcaaaac tttgtcgatt
     7321 atgtgaacag cggtttttat aacaacacta cctttcaccg cgtcattcct ggctttatga
     7381 ttcagggcgg cggtttcacc gagcagatgc agcagaaaaa accaaacccg ccaatcaaaa
     7441 atgaagccga taacggcctg cgcaacacgc gtggcaccat cgcgatggcg cgtaccgctg
     7501 acaaagatag cgccaccagc cagttcttta tcaacgttgc cgataacgcc ttccttgacc
     7561 atggtcagcg tgatttcggt tacgcggtat ttggtaaagt ggtgaaaggc atggacgtgg
     7621 ccgataagat ttcccaggtg ccgactcatg acgttggtcc gtaccagaat gtgccgtcaa
     7681 aaccggtagt tatcctttcc gctaaagtcc tgccgtaatg atttctcgcg cgggcaatct
     7741 tgcccgcgct tctgctctcc cggcgtaacc cggatttgcc gcttatactt gtggcaaatg
     7801 gacacgttca gggaggcatc aagtgaagaa actcaccgat aagcaaaagt cccgtctctg
     7861 ggagcttcag cgtaatcgta atttccaggc cagtcgccgt cttgaaggcg tcgagatgcc
     7921 tttagtcact cttactgccg cagaggcttt agcgcgcctt gtagagctga ggaggcacta
     7981 tgagcgataa attcggcgaa gggcgcgatc cgtatcttta tccaggcctt gatatcatgc
     8041 gtaaccggct gaacatccgc cagcaacaac ggctggaaca ggccgcttac gaaatgacgg
     8101 cgctgcgtgc tgcgaccatt gagcttggtc cgctggtgcg cggtttaccg catttgcgaa
     8161 ctatccatcg ccagctgtat caggatattt tcgactgggc agggcaactg cgtgaagttg
     8221 atatttatca gggcgatacg ccgttctgcc actttgctta tatagaaaaa gagggcaatg
     8281 ccctgatgca ggatctggag gaagaaggtt atctggttgg cctggagaaa acgaagttcg
     8341 tcgagcggct ggcgcattac tattgtgaaa tcaacgtgct gcatcccttc cgggtgggaa
     8401 gtggtctggc acagcggatc ttcttcgagc aactggcgat tcatgccgga tatcaactga
     8461 gctggcaggg tatcgaaaaa gaggcctgga atcaggcaaa tcagagtggg gcaatggggg
     8521 atctcaccgc actgcagatg atatttagca aagtggtaag cgaagccggg gaatctgagt
     8581 aaaatagcgc ggttctttcg taccggagcc gccatgatcc tgcttattga taactacgat
     8641 tcttttacct ggaacctcta ccagtacttt tgtgaactgg gggcggatgt gctggttaag
     8701 cgcaacgatg cgttgacgtt ggcggatatc gacgccctta aaccacaaaa aattgtcatc
     8761 tcacccggcc cctgtacgcc agatgaagcc gggatctccc ttgacgttat tcgccactat
     8821 gccgggcgct tgccgattct tggcgtctgc ctcggtcatc aggcaatggc gcaggcattt
     8881 ggcggtaaag ttgtgcgcgc cgcaaaggtc atgcacggca aaacctcgcc gattacacat
     8941 aacggtgagg gcgtatttcg ggggctggca aatccactta ccgtgacacg ctaccattcg
     9001 ctggtggtgg aacctggctc attaccagcg tgctttgacg tgacggcctg gagcgaaacc
     9061 cgcgagatta tggggattcg ccatcgccag tgggatctgg aaggtgtgca gttccatcca
     9121 gaaagtattc ttagcgaaca aggacatcaa ctgctggcta atttcctgca tctctgattt
     9181 ccgattgcca ttcagtgatt ttttatgcat attttgtgat tataatttca tatttattta
     9241 tgcgtaacag ggtgatcatg agatggcaat tgaacaaaca gcaattacac gcgcgacttt
     9301 cgatgaagtg atcctgccga tttatgctcc ggcagagttt attccggtaa aaggtcaggg
     9361 cagccgaatc tgggatcagc aaggcaagga gtatgtcgat ttcgcgggtg gcattgcagt
     9421 tacggcgttg ggccattgcc atcctgcgct ggtgaacgcg ttaaaaaccc agggcgaaac
     9481 tctgtggcat atcagtaacg ttttcaccaa tgaaccggcg ctgcgtcttg ggcgcaaact
     9541 gattgaggcg acgtttgccg aacgcgtggt gtttatgaac tccggcacgg aagctaacga
     9601 aaccgccttt aaactggcac gccattacgc ctgtgtgcgt catagcccgt tcaaaaccaa
     9661 aattattgcc ttccataacg cttttcatgg tcgctcgctg tttaccgttt cggtgggtgg
     9721 gcagccaaaa tattccgacg gctttgggcc gaaaccggca gacatcatcc acgttccctt
     9781 taacgatctc catgcagtga aagcggtgat ggatgatcac acctgtgcgg tggtggttga
     9841 gccgatccag ggcgagggcg gtgtgacggc atcgacgcca gagtttttgc agggcttgcg
     9901 cgagctgtgc gatcaacatc aggcattatt ggtgtttgat gaagtgcagt gcgggatggg
     9961 gcggactggc gatttgtttg cttacatgta ctacggtgtg acgccggata ttctgacttc
    10021 tgcgaaagcg ttaggcggcg gcttcccgat tagcgccatg ctgaccacgg cggaaattgc
    10081 ttctgcgttt catcctggtt ctcacggttc cacctacggc ggtaatcctc tggcctgtgc
    10141 agtagcgggc gcggcgtttg atatcatcaa tacccctgaa gtgctggaag gcattcaggc
    10201 gaaacgccag cgttttgttg accatttgca gaagatcgat cagcagtacg atgtgtttag
    10261 cgatattcgc ggtatggggc tgttaattgg cgcggagctg aaaccacact acaaaggtcg
    10321 ggcgcgtgat ttcctgtatg cgggtgcaga ggttggcgta atggtgctga atgccggacc
    10381 ggatgtgatg cgtttagcac cgtcgctggt ggtggaagat gcggatatcg atgaagggat
    10441 gcaacgtttc gcccacgcgg tggcgaaggt ggttggggcg taaataagac gcatcaggca
    10501 aaatatgtcg gatgcggcgt gaatgcctta tccgatggtt tagcctgtat tacgccttcg
    10561 aatcccgcaa cttgcgactc agccaaatcc cgtgatgcgg tcgctgacgc catgccatcg
    10621 acgaaatggt gtgcatggtg ttcagatgac caataacccg ctgtaaatgc tgctccagcg
    10681 tacctgccgc gccttcgtgc ggctgcatct ccggcgcatc catgatattg gcatcgccag
    10741 aactacccgg ttcgtcatac tccagtcgct gctgacaacg ctgaatggcg atttcacaag
    10801 actgtaaata ctcttgtgcc agttcaggtg gcaatgcccg gtgttcccgc gccagcgtgg
    10861 tcatggcatt aatatgctca acaataaact ggctgtgcgt tacccacagt ttcatatctg
    10921 ccagataatg gctgttaaac gccggttcct gcatcgcctg attcaatgag ttatacagag
    10981 tgttatgtgc ctgatttacc cgcattcgct gccaggccag tggcgtaggt tgcggatcct
    11041 cgctaagaat caagcgaatc gcttcctgat aggcttctaa agcgtcatgg gcgtttttac
    11101 gcaataaccc gctctgccac tgcggccaca gccagacagt accgccgaaa gcaattaaac
    11161 aaccaataat ggtatcgata agacgcggaa ggatgtattg ctcgccgttc aaccacaata
    11221 gttgcagggt atacactgcg gtaatagtaa aaccgaccgt cgcccagccg tagtttttgc
    11281 gcaatatcag gtagctggcg agggtggtaa tcagcatcaa cgtcagggtg taaccttcgg
    11341 gaattttaaa gtgcagcgcc acgcccgcaa tgattaaccc gaccacggtt cccacggagc
    11401 gattcacaat cctcagacgg gttgcgccat agccattttg tgtcaccagc aataccgtca
    11461 tcaggatcca gtacgacttc ggcagatgca gcgcggtgcc catcaggctg gcaacgctta
    11521 acatcacact gagtcgtccg gcattgcgta gcgccggaga ctttagtgac agataacttt
    11581 tcagcgcagg aagtaatggc attcgccgct gtttatcggc cagtaagtca cgggcataga
    11641 gcggtttttg ggtgcgcagc acgcgggcga tgcggctgaa atgccagtag cagaattgcc
    11701 caaccggatt atccggatgc tggcgggcga ttttttccag tgcgccaatt tgcttttcca
    11761 tcgtaaaacg cgttggcagg cgatggtaaa gaatgtcatc agccagcacg cgcaggcgag
    11821 cggcgacggt ttgcgcattc cagcggataa cttcttccgc atggctacgc tcgaccagct
    11881 tttgcacctc ttccggctga tgcaaactga ccgaaatatg ttcctgtaaa tccagcgcct
    11941 cctggaaaat acgcagcatc cgcttgtagt cagtattatt ttgcgcggaa agcatatgca
    12001 tttgctgata gcactgggta attagatcga ccgctttttg ctggcgcacc agcagcggcg
    12061 gcagcgcttt ttcagggtcg gtgtgctggg taagcaggct gtatttggct tcacaataat
    12121 ctgccagttc acggtacagc agacttagtg actcgcgcag cggttgttcg cgccagatcc
    12181 agaaccaaaa ccagttaaac aatccgtacc agagagtgcc caacgcatag atgagcaacg
    12241 gttcccagac cggcatgtat cccgccagac tgagggtaaa aatggctgcc agcagcgatg
    12301 cgggaagcaa ttttgcgtgc aatggcccca actcagcagt gacgccaagt accagcgtta
    12361 atccggtcag caaaaagggc aggggaacat cttttgccag tagcagctgt gtcagtaagc
    12421 tacaggtggc aaacagcgac gcaccaatga ttaagcgttt gaaaaaacgt ttatgaggcg
    12481 tatcaaggcc cgcaatattg caacaggcag gaacgagaga gaagagcaga ccgaatcgta
    12541 attcgccaag cattaaccca acggccacgg gcaaacatag caccagcgtt tgtcgaagtg
    12601 catagttgat atcggggtga taaatcagtc tgcgccacat cgggggaaac aaaatggcgc
    12661 gctaccaggt aacgcgccac tccgacggga ttaacgagtg ccgtaaacga cgatggtttt
    12721 accgtgtgcg gagatcaggt tctgatcttc aagcatcttc agaatgcgtc ccacggtttc
    12781 acgagaacag ccgacaatct gaccgatttc ctgacgggta attttgattt gcataccgtc
    12841 cgggtgagtc atagcgtctg gttgttttgc cagattcagc agagtctgtg caatgcggcc
    12901 cgtcacgtcg aggaacgcca ggttgcccac tttctctgaa gtgacttgca gacgacgcgc
    12961 catctgtgca gacagacgca tcagaatgtc cgggtttacc tgaatcaatt ggcgaaattt
    13021 tttgtacgaa atttcagcca cttcacaggc ggttttcgca cgtacccatg cgctacgttc
    13081 ctggccctct tcaaacaggc ccagttcgcc aataaaatca ccctgattca gataggagag
    13141 gatcatttct ttaccctctt cgtctttgat cagcactgcc acagagcctt taacgatgta
    13201 gtacagcgtt tccgcttttt caccctggtg aataagcgtg ctcttggatg ggtacttatg
    13261 aatgtggcaa tgagacaaga accattcgag agtcgggtct gtttgcggtt tgccaagcac
    13321 catgcgcggt tatcctctgt tataagcttt ctccagagcc agataacgcc gctgtctctg
    13381 gattgccgaa atatgcttcc cgctacctgg gaaggggcta tcaactgtac tgcacggtaa
    13441 tgtgacgtcc tttgcataca tgcagtacat caatgtatta ctgtagcatc ctgactgttt
    13501 tagcatagct ttcgctttgt gtctcctggt gtctcgcttc agcatgaccc aggtcgcctt
    13561 ccgttgcgcg atttggttag tacg
//
LOCUS       DADLFA010000107        13518 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000107 DADLFA010000000
VERSION     DADLFA010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13518
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.107"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..184
                     /locus_tag="JQH65_002648"
     CDS             <1..184
                     /locus_tag="JQH65_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY6140020.1"
                     /translation="WSLAAGANPSFIASQMGHTNAQMVFNVYGAWMKDNNHEQIELLN
                     RRLSESVPCMPHKKAG"
     gene            205..906
                     /gene="mlrA"
                     /locus_tag="JQH65_002649"
     CDS             205..906
                     /gene="mlrA"
                     /locus_tag="JQH65_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HAY6140021.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYP
                     AQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARRKQGKDALVVGW
                     NIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQL
                     TSWQEQGHDIFPLGI"
     gene            966..1073
                     /locus_tag="JQH65_002650"
     CDS             966..1073
                     /locus_tag="JQH65_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HAY6140022.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(1054..1785)
                     /gene="yehW"
                     /locus_tag="JQH65_002651"
     CDS             complement(1054..1785)
                     /gene="yehW"
                     /locus_tag="JQH65_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HAY6140023.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFVVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(1790..2716)
                     /gene="yehX"
                     /locus_tag="JQH65_002652"
     CDS             complement(1790..2716)
                     /gene="yehX"
                     /locus_tag="JQH65_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HAY6140024.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVCREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLEEA"
     gene            complement(2709..3866)
                     /gene="yehY"
                     /locus_tag="JQH65_002653"
     CDS             complement(2709..3866)
                     /gene="yehY"
                     /locus_tag="JQH65_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HAY6140025.1"
                     /translation="MTYFRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPGKKGSICALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(3873..4790)
                     /gene="osmF"
                     /locus_tag="JQH65_002654"
     CDS             complement(3873..4790)
                     /gene="osmF"
                     /locus_tag="JQH65_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HAY6140026.1"
                     /translation="MPLSKVWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDSEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQISGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(5042..7339)
                     /gene="bglX"
                     /locus_tag="JQH65_002655"
     CDS             complement(5042..7339)
                     /gene="bglX"
                     /locus_tag="JQH65_002655"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HAY6140027.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPFTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDMVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            7535..9250
                     /gene="dld"
                     /locus_tag="JQH65_002656"
     CDS             7535..9250
                     /gene="dld"
                     /locus_tag="JQH65_002656"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HAY6140028.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPSSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALQRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(9289..10221)
                     /gene="pbpG"
                     /locus_tag="JQH65_002657"
     CDS             complement(9289..10221)
                     /gene="pbpG"
                     /locus_tag="JQH65_002657"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HAY6140029.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAAITASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(10395..10982)
                     /locus_tag="JQH65_002658"
     CDS             complement(10395..10982)
                     /locus_tag="JQH65_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HAY6140030.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            11152..11730
                     /locus_tag="JQH65_002659"
     CDS             11152..11730
                     /locus_tag="JQH65_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY6140031.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(11860..12621)
                     /locus_tag="JQH65_002660"
     CDS             complement(11860..12621)
                     /locus_tag="JQH65_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY6140032.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(12674..>13518)
                     /gene="mdtQ"
                     /locus_tag="JQH65_002661"
     CDS             complement(12674..>13518)
                     /gene="mdtQ"
                     /locus_tag="JQH65_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311052.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HAY6140033.1"
                     /translation="AALNTVLQQIEKEQNTIIATDRQLYQNGITSSVEGVETDINASK
                     TRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKPVALPKVASQLPDELGYSLLARRAD
                     LQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQDALHLSDLFRHSAQQMGVTAGLTL
                     PIFDSGRLNANLDIAKAESNLSIASYNKAVVEAVNDVARAASQVQTLAEKNQHQAQIE
                     RDTLRVVGLAQARFNAGIIAGSRVSEARIPALRERANGLLLQGQWLDASIQLTGALGG
                     GYKR"
BASE COUNT         3237 a         3779 c         3414 g         3088 t
ORIGIN      
        1 ctggtcactt gccgcaggag ctaatcctag ttttatcgca agccagatgg gccacacaaa
       61 cgcacaaatg gtattcaatg tttacggagc atggatgaaa gacaacaatc acgaacagat
      121 agaacttctt aacagaagac tatctgaaag tgtcccatgc atgccccata agaaagcggg
      181 gtaaaataaa aacttgcaaa atcaattggt ttacctttaa tccctgtcac gttacgcgcg
      241 tggcagaggc gttacgggtt gctgaaaccg caacggacag acggcggtca tcgactgttc
      301 aacgatgccg atattgaccg gatccgcgag atcaaacgct ggatcgacaa cggcgtgcag
      361 gtcagcaaag ttaaaatgct gctcagtaat gaaaatgttg atgtgcagaa cggctggcgc
      421 gatcagcaag aaacattact gacctacctg caaagcggca atctgcatag cctgcgaacg
      481 tggatcaaag agcgcggtca ggattacccc gcccagacac tcaccacaca tctgtttatt
      541 cctctgcgcc gacggcttca gtgccaacaa ccgactctcc aggcgctgct ggcgatcctc
      601 gacggcgtac tgatcaacta catcgccatt tgtctggctt cggcacgtag aaaacagggt
      661 aaagatgcgc tggtggttgg ctggaatatt caggatacca cccgtctgtg gctggagggc
      721 tggattgcca gtcaacaagg atggcgcatt gatgtcctcg cccactcgct caatcaacta
      781 cgccctgaac tattcgaagg ccgtacattg ctggtgtggt gcggtgaaaa tcgaacctcc
      841 gcccaacagc agcaactcac cagttggcaa gaacaaggcc atgatatttt cccactcggc
      901 atttaatgat tcgttaacaa atgcgcttta ctgtacaatc ctttcgttaa cataaggagt
      961 gcaatatgcg catagctaaa attggggtca tcgccctgtt cctgtttatg gcgttaggcg
     1021 gaattggtgg cgtcatgctc gcaggttata cctttatttt gcgtgctggc taagcgcctg
     1081 caccagcctt tcaaacaggc ggtctgcaat gatcgccgcc agtgccacca gtaacgcccc
     1141 ctggatcaca tacgcggtat taaatccgct aagcccgatg atgatgggcg tacccagcgt
     1201 gctggcccct accgttgagg cgatcgtcgc cgtaccaatg ttgataatca ccgaagttcg
     1261 cacgcccgcc agaatcaccg gagccgccag cggtagctcg accttacgca gtcgctgacc
     1321 acgactcatt cccatacctt tcgcaacttc tgtcacgctg gcatcaatcg ctcccagccc
     1381 ggcaagtgtc gcctgcagga cgggcagcac accgtaaagg atcaaggcga taatcgctgg
     1441 ttgcagacca aagccgatca ccggaacggc aatcgccagc actgcgacag gcggaaaagt
     1501 ctgtccaacg gcggcaatag tttccaccag tgggcgaaat tccgcgcccc acgggcgagt
     1561 gacagcaatt ccggcaccag tgccaatgat caccacaaac aaactcgaaa ttcccaccag
     1621 ccagaaatga gccagtgcca gagctgcaaa actttcttgc tgataaacgg gtcgtggcag
     1681 ttgtgggaac aaggcagcaa acagcggctg gctgtaaggc agccagaaaa tcagtgccac
     1741 aaacaaagca atgagccaga acagcggatc gcgcaacatc ttcatacgct tacgcctcct
     1801 ccagcagatc ctgaaaatgc agcgtgccgc aaggctggcc ctgcgtgttc accaccggca
     1861 gcacctcgca tccccgcgcg acaaacaggg agagcgcatc gcgtagcgtc atctcttctg
     1921 ccagtgcctc accttctgcc cgttcttcgc gacacacgta atccgccaca ctacgtaacg
     1981 aaagcaggcg cacacccagt tcgctgcgcc caaaaaactg gcggacaaaa tcgtttgccg
     2041 gacgagtcag catcgtcagc ggattgccct gctgcaccac ttcaccgtga tccatcaata
     2101 ccagatgttc tgccagccgt agcgcctcat caatatcatg agtgaccagc acaatggtac
     2161 gccccagcaa acggtgaatg cgcgtcatct cttgttgcaa cgcgccgcgc gttaccgggt
     2221 ccagtgcgcc aaaaggttca tccatcagta agacttgcgg atcggcagcc agtgcgcgcg
     2281 ccactcccac acgttgctgc tgaccaccgg aaagctgatg cggataacgc tcacgtaaat
     2341 ttgactccag ccccagtagc gccattaatt cgtcgatacg atcgtcaatc cgcgcccgcg
     2401 accatttttg taattgcggc acggtagcga tgttttgcgc cacgctccag tgggggaaca
     2461 ggccaataga ttgaatggca tagcccatcc ggcggcgcaa ctccagtact ggcagcgagc
     2521 gaatttcttc tccggcaaag cggatcactc cgctgtcatg ctccaccagg cggttaatca
     2581 ttttcagggt ggtggatttg ccggagccag atgtgccaat cagcaccgaa aaactccctt
     2641 cctgaaaatt gagattgaga tcgttaacgg ctttttgtgc gccgaacagt ttgctgacat
     2701 ggctaaattc aatcattacg tttcaccttc agcagtgcga taagcaaatc gaacagcgcg
     2761 tcggtaagca ccgccagaac aattaccggg atcaccccca gcaacactaa atcaatggcg
     2821 ctgcttagca gcccctggaa aaccagcgca ccaaaaccgc ctgcgccgat taacgccgca
     2881 atcaccgcca tacctacagt ttgcaccatc accacccgca ggctgcgcag aaataccggt
     2941 aacgccagtg gtaactgaac atgcaggaat cgctgcgccc cgctcatgcc catcgctctg
     3001 gcactctcca gcacatcgcg cgggatctgg ttcaaaccga ctaccacgcc gcgcaccagc
     3061 ggcagcaagg catagagcac cagcgcaatc agtgcgggtg tcattccggt tcctgctatg
     3121 ccgagcttcc ccagccacgg aaaggccgtc accagcgcgg caagcggcgc aatcaacagg
     3181 ccaaagagcg ccaccgaagg cacggtctga atgacattga gcagagaaaa aattgccccc
     3241 tgccgagcag tggaaaagta gcaccagatg cccaacggca caccaatcac taacgcaggc
     3301 agcaccgcac caaacagcaa cgtcagatgt tgtgccagcg cgtcgtcaaa cacatcctga
     3361 cggttggcgt attctttcat tagtgagaga tcgttaagcg tgccggagta cagcaaccac
     3421 agcggaataa tggcaatctg catatgcaac aaccagcgcc acagcggatg cgtggagatt
     3481 cggcggatgg catcgctaca ggccagcaat gccagcgccg cagccagcca gaaaccactg
     3541 ccgaggctgg tacgcgccag cgcactgcca ttttgcgcca gttgtgtcgc cgcctttccc
     3601 gcgccccaca ccagcaatac gaagacgaat tgcgccagaa tgagtgcaca aatgctgcct
     3661 tttttaccgg gaataaaaca ggccgtcagc caggcgcaac caacgcccac cagcatccag
     3721 atcgtttgcg gccacagctg ccagagatga cgcccctcac ccgaaactaa acgattaggc
     3781 gcgtaactga taaacggcag cgccgctgcg attgccgtca gcaacagcag cagcgccaga
     3841 acaggattaa tacggaaata agtcacggga aattacttcg tccacccttt ttgtttcagg
     3901 tagtcggcag ccactttttt ggcatccagt ccttccacag caatgctggc attcagttgc
     3961 tgcaatgttt ttgcatcgag gctggcgaag actggctgta gccactgtgc catttgcgga
     4021 tactccttca acaccgactc acgcaccact ggtgcaggcg cgtagatagg ttgcacacct
     4081 tgcggatcgc ttaaggtttg cagccccagc gccgctaccg ggccgtcagt gccgtaagcc
     4141 attgcagcat taacgccaga gatttgctgg gcagcggctt tgatcgtcac cgccgtgtcg
     4201 ccaccagcca gtgacagcaa ctgatcctga ccgagcttaa agccataggc tttttcaaac
     4261 gcgggtaacg catcggcgcg ttcgataaac tctgccgagg ctgccagttt gaaggtgccg
     4321 ccctctttca gataacgact caggtcggca agcgaagtga gtttattttt ctctgccaca
     4381 tcctgacgca cggcgatagt ccaggtgtta tttgcaggcg cgggcgtcag ccagattaac
     4441 ttgttgtgct ccgaatcgag ctttttgact ttctcgtaac cttgctgcgc gtttttccac
     4501 gctgcatcgt tttcatcttt aaagaagaaa gcgccattgc cggtatattc cggatagata
     4561 tccagttcac cggaagtaat cgccccgcgc accacaggag tcgttccgag ttgcacttta
     4621 ttcaccgtcg gtacgccgtg gctttccagc acctgcaaga tgatgttgcc gagtagcgcg
     4681 ccttcggtat cgatttttga accgacttta acgggggaag ccgcctgtag cggcaggctc
     4741 acggctgcca acataaccag tgaacctgcc cagacctttg agagtggcat gatgctttcc
     4801 tcattctttt actgttgttt tcagcgaatt aagagaaaag catagttgat aatgacagag
     4861 ttagcctgag cggaaggatt cagttgcaga atcagataaa tacttaaagg gcatattttg
     4921 cccggcgttt cttgccggat gcggcgtaaa cgccttatcc ggcctacaaa ggtcgaaaat
     4981 tcagcaagtt aagatatgaa catcaggcaa agggcgcaaa cgtcgcgccc ttactaaagc
     5041 attacagcaa ctcaaactcg cctttcttaa cgcgtgcgga atcagtgccg ataaagacat
     5101 tgaacttgcc aggctcggcg tcatatttca tctgttgatt ccagaacttc agcgcctcga
     5161 tatcaatcgg gaagctgaca gtctgagttt cgcccggttt cagggtgatt ttctcaaagc
     5221 ctttcagctg cttcactgga cggctcatgg aagccgtcac atcctgcaag tacatctgta
     5281 ctaccgtggc cccttcgcgt ttaccggtgt tcgtcacctg cacgctggcc gtcactttgc
     5341 cgtcacgctt catggtcggc gcagaaagtt tcacatcaga gacggtgaaa gtggtatagc
     5401 tcagaccata gccgaacgga taaagcgccc cgttagcttc atcaaaataa cgcgaagtgt
     5461 atttgttcgg cttgtcggca ttatacggac gaccggtgtt cagatggctg tagtacaccg
     5521 ggatctgccc gacagaacgc gggaaggaca tcggcagctt gccggacggg ttgtaatcgc
     5581 caaacaacac atcggcaatt gcattaccgc cttcagtccc cgcaaaccag gtttccagaa
     5641 tcgcatcagc ctgctgatct tctttcacca gcgccagcgg acgcccgttc atcagcacca
     5701 gcaccagcgg tttaccggtg gctttcagcg ccgcaatcaa gtcacgttgg ctttgcggaa
     5761 tagtgatatc ggtacggctg gaggcctcgt gcgccatccc ctgtgcttca ccgaccacag
     5821 ccaccaccat atcagattgt ttcgccgtct gcaccgcttc atcaatcatc tcttgcggcg
     5881 agcgcggatc gactttgacc gcttcttcat actgattcag gaaatcgata atgcctttgt
     5941 cactggtaac gttcgcccct ttggcataca gcactttacc gttttcaccg acggcatttt
     6001 taatcccggt cagtacggtc acggattgat cggcaacacc ggctgcggac cagctgccca
     6061 tcacgtcgcg tttactgtcc gccagtggcc caaccaccgc aatggtggcc gattttttca
     6121 gcggtaacgt ttcgagacgg tttttcagca acaccaggct ttcgcgcgcc acttcacgtg
     6181 cttctttacg gtgcaggcgg ctttcggcat tggtatccac cgggtcagac tctttcggcc
     6241 ccaaatggct gtatgggtcg ttaaacaatc ccatatcata tttaacgttc agtacatggc
     6301 gggcagcatc gtccagctct tccatcgtca ctttgccgga tttgatcaac ccaggcagat
     6361 acttcgagta atactcgtcg ctcatactca tgttgattcc ggatttcagc gccacgcgca
     6421 ccgcatcttc cgggtctgcc gccgtgccat gtttaatcag ctctttgatt gcaccgtgat
     6481 cggaaacggt gatgccttta aagccccact ggtcgcgcag aacatctttc agcagccagg
     6541 aatcggaggt ggctggcgtg ccgttcagcg agttcagcgc caccatcacc gcgccgctgc
     6601 ctgcgtccag ccccgctttg tacggcggca tataatcatt aaacaggcgc tgcggactca
     6661 tatcgacggt gttgtactct ttaccgcctt ctaccgcgcc gtatgcggca aagtgtttga
     6721 cgctggtcat caccgagtag cgatctgccg ggcttttacc ctgcatcgct tccaccatgg
     6781 ttttacccat tgttgaggtg agatacgtat cttcgccaaa accttcggaa gcacgtcccc
     6841 agcgcggatc gcgcgagaca tcgaccatcg gtgcccaggt catattcagg ccatcatctg
     6901 ccgcttcata agcagagaca cgtccgaccg ttttcactgc atcgaggtta aaagacgagg
     6961 ccagaccgag gctaatcggg aacaccgtgc gctgaccgtg cagcacgtcg taagcaaaga
     7021 aaagaggaat tttcaggcgg cttaattcca tcacctgatc ctgcatggcg cggatatcct
     7081 gacgggttac ggtgttgaaa atcgccccaa cctgaccgtc tttgatcatc tcgcggatcg
     7141 cctctttcgg gttatccggg ccgacgctga ttaagcgcag ctgaccaatt ttctcatcaa
     7201 ctgtcatttt cttaagcaat tcggtgacga acgcatcccg cgcttcgggc gtgaatggat
     7261 ggttgccgaa taaatcatcc gccagtgctg gctgcagggc cagactcacc gcgattccta
     7321 ctgaacatag ccatttcata tggatttttc ttcctctgtt gcccgacgag gcagcgaaaa
     7381 tggtgcaaaa accgtagttt gccataagca tgatggagag agaaaaagaa tgctcagttt
     7441 attgtctgaa ttttcaaaat attcactcgc tgaattgtca tacaaggcgc tattctagtt
     7501 tgtgatattt tttcgccacc acaaggagtg gaaaatgtct tccatgacaa caactgataa
     7561 taaagccttt ttgaatgaac ttgcccgtct ggtcggtcat tcacacctgc tcaccgatcc
     7621 cgcaaaaacg gcccgctatc gcaagggctt ccgttctggt cagggcgacg cgctggctgt
     7681 cgttttccct agctcactac tggaattgtg gcgggtgctg aaagcctgtg tcaccgccga
     7741 caaaattatt ctgatgcagg ctgccaatac aggcctgacc gaaggatcga cgccaaacgg
     7801 taacgactat gatcgcgata tcgtgatcat cagcaccctg cgtctcgaca agctgcacgt
     7861 tcttggcaag ggcgaacagg tgctggccta tccgggcacc acgctctatt cgctggaaaa
     7921 agccctcaaa ccgctgggac gcgaaccgca ctcagtgatt ggatcatcgt gtataggcgc
     7981 atcggtcatc ggcggtattt gtaacaactc cggcggctca ctggtgcaac gtggcccggc
     8041 gtataccgaa atgtcgttat tcgcgcgtat aaatgaagac ggcaaactga cgctggtgaa
     8101 ccatctgggg attgatctgg gcgaaacgcc ggagcagatc cttagcaagc tggatgatga
     8161 tcgcatcaaa gatgacgatg tgcgtcacga tggtcgtcac gcccacgatt atgactatgt
     8221 ccaccgcgtt cgtgatattg aagccgacac gcccgcacgt tataacgccg atcctgatcg
     8281 gttatttgaa tcttctggtt gcgccgggaa gctggcggtc tttgcagtac gtctagatac
     8341 cttcgaagcg gaaaaaaatc agcaggtgtt ttatatcggc accaaccagc cggaagtgct
     8401 gaccgaaatc cgccgtcata ttctggctaa cttcgaaaat ctgccggttg ccggggaata
     8461 tatgcaccgg gatatctacg atattgcgga aaaatacggc aaagacacct tcctgatgat
     8521 tgataagtta ggcaccgaca agatgccgtt cttctttaat ctcaagggac gcaccgatgc
     8581 gatgctggag aaagtgaaat tcttccgtcc gcattttact gaccgtgcga tgcaaaaatt
     8641 cggtcacctg ttccccagcc atttaccgcc gcgcatgaaa aactggcgcg ataaatacga
     8701 gcatcatctg ctgttaaaaa tggcgggcga tggcgtcggt gaagccaaat cgtggctggt
     8761 agattatttc aaacaggccg aaggcgattt ctttgtctgt acgccggagg aaggcagcaa
     8821 agcgttttta caccgtttcg ccgctgcggg cgcagcaatt cgttatcagg cggtgcattc
     8881 cgatgaagtc gaagacattc tggcgttgga tatcgctctg cagcgtaacg acaccgagtg
     8941 gtatgagcat ttaccgccgg agatcgacag ccagctggtg cacaagctct attacggcca
     9001 ttttatgtgc tatgtcttcc atcaggatta catagtgaaa aaaggcgtgg atgtgcatgc
     9061 gttgaaagaa cagatgctgg aactgctaca gcagcgtggc gcgcaatacc ctgccgagca
     9121 taacgtcggt catttgtata aagcaccgga gacgttgcag aagttttatc gcgagaacga
     9181 tccgaccaac agtatgaatc cggggatcgg taaaaccagt aagcggaaaa actggcagga
     9241 agtggagtaa aaattacgga tggcatagta tcgccatccg tcattcactt aatcgttctg
     9301 tgccgtctgc cccgccgccg ccatttgggc ggctttttgt tttttatagc tcaacgctgc
     9361 tgctggaaca ggcatcactt taccggtttc aatccaggta cgcaggcggc tggcatcggc
     9421 aaaatgggta tatttgccaa acgcatccat cactaccagc gccaccggtt tattattgat
     9481 aaccgtacgc atcaccagac aatggcccgc cgcattggta aaaccggttt tggttaactg
     9541 aatattccag ttatcgcgat acaccagatg gttagtattg cggaacggca gcgtatacgt
     9601 cgggttagag aaggttgcca tatcttcccg ggtagtactt aactgcccga tcaacggata
     9661 ttgtttgctg gcaatgagca gtttggttaa gtcacgggca gttgaaacgt tatgcaccga
     9721 caatccggta ggttcaacaa agcgcgtgtt gttcattccg agcgatttcg ctttcgcatt
     9781 cattgcctta ataaaggctt tgtaaccacc gggataatgg tgtgcaaggc ttgccgccgc
     9841 gcggttttct gaagacatca gcgccagcaa cagcatatct ttacggctga tttcgctatt
     9901 cagtcgtacg cgcgaataga cccctttcat ctccggcgtc tggctgatat ccacttttag
     9961 tttttcatcc agcggcagtc gtgcatccag cacaaccatc gcggtcatta atttgctgat
    10021 agacgcaatc ggacgcacca gatccgggtg gttcgagtag atcactttgt tggtattcag
    10081 atcaacaatc atcgcgctac cggaggcaat ttcaggttgt gaagcggtga tagcggctgc
    10141 cgttttcgca acggcctgcg gtgccaaagg cacagccagc atcagggcca ggctaaataa
    10201 agaaactcga aatttcggca tgatgagcat tcagatagtg gttcacgcgc acaggttgcg
    10261 caccgccgga gtaaggattt actgaggcta gcgacgccat cataacgagc aaaaagtgcg
    10321 atcgtcaaag gagaatcgtg aggaaatgct gcattgctga catttacgcc agcaatgcaa
    10381 cgtcaaagaa ctttctagaa caaacgataa ccgtagcccc atagtataac ggttagggcg
    10441 agcagcactt ccagtaccag cacgccaatc gccagcgtcg aactagagaa gctaaggcct
    10501 tcctctttgt tgatattcag gaagctcgga ataccaaggt aaagcaggta gccggtgtaa
    10561 aacagcgcca ccgtgccgac cagcgcacac aaccagacca gtggataaag cgccaccaaa
    10621 ccgcttaaaa acagcggagt tgcaacgtag cccgcgaaga ccatgcagtg cgcaagtgac
    10681 ggacgctgcg gataattacg cgccatccac cagatgaccc gccccattac cgcaacccca
    10741 gccagcataa cgccataaaa caggacagcc agcgccagtc cggtaaacca ggataacttc
    10801 aggatagtgc catcgccaaa attccagcca atctgtgtag tgccaatgaa ggcgcaaatc
    10861 accggaatcg ccgccatcag caaaacgtgg tgggtgtaat ggtgagaaat cgtttcgttt
    10921 tcgcgattaa tcacctgcat ttcacgatcg ggatgggaaa acagtcccca gacatggctc
    10981 ataccgcccc cttgttgtga gttcatgaac ctgacagttc aagtataagt cagcttgtga
    11041 ttattttttg ttcgctcatg taatttgcac cgtctgatgt acgtcctttt tcagcgtgaa
    11101 tgatttccag ggtgtatgct tacaggcaac caaagggaga cagactggcc tatggatctc
    11161 aatacactca tctcacaata tggttatgcc gcgctggtga tcggtagcct ggcggaaggt
    11221 gaaaccgtga ctttgctggg aggcgttgcg gcacatcagg ggctattaaa gttcccgctg
    11281 gtggtacttt ctgtggcgct tggcggcatg attggcgacc aggtgcttta tctgtgcggg
    11341 cggcggtttg gcggcaagct gttacgccgt ttctcgaaac atcaggataa aattgagcgg
    11401 gcgcagaaac ttatccaacg ccatccgtat ctgtttgtca ttggtacgcg ctttatgtat
    11461 ggctttcggg tgattggccc gacgctgatt ggtgccagcc agctgccgcc gaaaatcttt
    11521 ctgccgctga atattctcgg cgcatttgcc tgggcgttga tttttaccac tattggttac
    11581 gctggtggtc aggtgattgc gccgtggttg cacaatctcg accagcattt gaagcactgg
    11641 gtctggttga ttctggtcgt ggttctggtg gtgggcgtgc gctggtggct gaaacggcgc
    11701 gggaagaaaa agccggatca tcaggcgtaa aaccattgcc ctgggtaagg cgttcgcgcc
    11761 gcatccgaca tccggtgcac catgcctgat gcgacgcttg tgcgtcttat catgcctacc
    11821 aaaattttac ccattgaaat aaccacgcac ctcactgcgc tattctgggt tgaactgcgg
    11881 attcgccaac ataaagccgc catccactat caacgactgc ccggtggtgt aatttgcgcc
    11941 ctctgagcac agccacgcta ccaggctggc aatctcatgc gttgcgccaa aacgccgcag
    12001 aggaatcgaa ggctccgcgt cgggcttcac gtcgctgtca tccatgccat tcattggcgt
    12061 ggcgatcgcc ccaggcgcga ctgcgttcac caaaatctta tgcctgacca gctccagcgc
    12121 catcgctttg gttaacccac cgagcgcatg tttagcggct gtgtaggcgc tggcatccgg
    12181 cagcggcgta tgttcatgta ccgatgtaat gttgatgatg cgaccgccct gcccttgttt
    12241 caccatctgg cgggccgcaa tttgtgagca taaaaatgca ccatcgacat caacggtaaa
    12301 aatcttgcgc cactcatcga aagccatatc aagaaacggc gctttggtca ttgcgcccgc
    12361 attattcacc agcacatcaa tacgccccag ccgttgaatg agtttctcca gcgcctgcgc
    12421 accttctggc agtttaccga gatccagctg cacgatctcc gcacgtacgc cgtggctaac
    12481 tacctcacgc gcggtatctt ttgccccttc ttcatctgag tgccaggtaa tgccaatatc
    12541 aaacccttgc tgcgccagta ataacgcgca ctctttgccg atccccgaat cggaggcggt
    12601 aataatcgca acctgtgcca tcgagttctc cacttaacgc tgaataaacg ttaagtatag
    12661 aaggcgcata tcatcagcgt ttgtaacccc cgcccaacgc accagtgagt tgaatggagg
    12721 catccagcca ctgcccttgc aataacaggc cattggcccg ctcacgcagc gcggggattc
    12781 tggcttcgct gacgcgggaa ccagcaatga tgcccgcgtt aaagcgcgcc tgcgcaagac
    12841 ctaccacacg caaggtatcg cgctcaattt gcgcctgatg ctggtttttc tccgcaagtg
    12901 tctgaacctg actggctgcc cgcgccacgt cattcaccgc ttcaaccacc gctttgttgt
    12961 agctggcgat agacaagttg ctttcggctt ttgcgatatc gagattggcg ttaagacgac
    13021 cactatcgaa aatgggtagc gtcaggcctg ccgtaacgcc catttgctgc gcggaatgac
    13081 ggaacagatc gcttaagtgc aacgcatcct gttgcaggaa ggccatcagg ttgatgtcag
    13141 gataaaatgc cgcttttgcc gcatcaatgg tgcttagcga tgactcaacg taccagtgcg
    13201 ccgcctgcaa atctgcccgc cgggccagta aggagtaccc cagttcatca ggaagctggc
    13261 ttgccacttt cggcaacgcg accggtttaa gcttcaatga ctttgtctgg ttatttgtaa
    13321 gtgcgcttaa ccgtgcctca ataattttca ttttccccgc gacatcgttg agctgctgcc
    13381 gggttttgct ggcattaata tcggtttcca caccttcaac tgaagaagta atcccgttct
    13441 gatatagctg gcgatcggtc gcgataatgg tgttctgctc tttttctatt tgctgcaaga
    13501 ccgtgtttaa cgccgcct
//
LOCUS       DADLFA010000108        13490 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000108 DADLFA010000000
VERSION     DADLFA010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13490
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.108"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            558..1646
                     /locus_tag="JQH65_002662"
     CDS             558..1646
                     /locus_tag="JQH65_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAY6140034.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLIKHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            1657..3936
                     /locus_tag="JQH65_002663"
     CDS             1657..3936
                     /locus_tag="JQH65_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6140035.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEVDDSPDTQEESQALLLRATRMDNSDTLWDHLFEDESQQIALPSALAHYFA
                     QLRGDFPGDALNRQREAFMARWIAWAVQRNNGDVLVVCGGWHAPALAKMWRECPQEIN
                     KPELPSLANAVTGCYLTPYSEKHLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHNLPASIADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFTPDGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEEHWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVNLSGVSLSAAVALLERRSQAIHAPALDRGAALGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DANFINALPDLRAVMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            3929..5065
                     /locus_tag="JQH65_002664"
     CDS             3929..5065
                     /locus_tag="JQH65_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAY6140036.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWFNSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIPSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            5062..7062
                     /locus_tag="JQH65_002665"
     CDS             5062..7062
                     /locus_tag="JQH65_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6140037.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANGQALKEAQCSCGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSGRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLTAV
                     GFYRLAHVLVQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            7187..7648
                     /locus_tag="JQH65_002666"
     CDS             7187..7648
                     /locus_tag="JQH65_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HAY6140038.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASISATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(7689..8159)
                     /locus_tag="JQH65_002667"
     CDS             complement(7689..8159)
                     /locus_tag="JQH65_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAY6140039.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(8206..8925)
                     /gene="yehT"
                     /locus_tag="JQH65_002668"
     CDS             complement(8206..8925)
                     /gene="yehT"
                     /locus_tag="JQH65_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HAY6140040.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(8922..10607)
                     /gene="btsS"
                     /locus_tag="JQH65_002669"
     CDS             complement(8922..10607)
                     /gene="btsS"
                     /locus_tag="JQH65_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HAY6140041.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLQLPWRDEA"
     gene            10829..10894
                     /locus_tag="JQH65_002670"
                     /pseudo
     CDS             10829..10894
                     /locus_tag="JQH65_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            11122..11370
                     /locus_tag="JQH65_002671"
     CDS             11122..11370
                     /locus_tag="JQH65_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HAY6140042.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(11486..11755)
                     /locus_tag="JQH65_002672"
     CDS             complement(11486..11755)
                     /locus_tag="JQH65_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY6140043.1"
                     /translation="MLLQHSDIEIAWYASIQQEPNGWKTVTTQFYIQEFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNCVDTSTAPDIEWPEEPDTM"
     gene            11812..12480
                     /locus_tag="JQH65_002673"
     CDS             11812..12480
                     /locus_tag="JQH65_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY6140044.1"
                     /translation="MVKLMKKNTDDGAKIYTPITLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFETYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            12796..>13490
                     /locus_tag="JQH65_002674"
     CDS             12796..>13490
                     /locus_tag="JQH65_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY6140045.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTA"
BASE COUNT         3429 a         3445 c         3285 g         3331 t
ORIGIN      
        1 ctgtattccc ccttagatgc gtttcaagtt gcccgttact tcaacaaatc aatcaaccgt
       61 aacagagcca ctaatagctc cagtacagca atgagcacgg ttaacttttg tgtcagcgtg
      121 tccatccgct ctccttatta aggagcgcaa caccagcacc ttacccttac actgcctgtc
      181 gatactggtt tacgtatgtt ggaccacgat gttgcactcc ggatcagggc accacctgca
      241 gcaccacccg tccggtagcc aggacttgaa atagattcga tccggcgcaa cgtcccaacg
      301 gtctggatta taaatctcat tatcttaatt gcaacggggt ccagccgtgg ttataattcg
      361 ttcgttggaa cacgatgttg cacaggctgt ggtgttaggc ctgaaagtag taaataaaag
      421 aacctgcctc accagcaggt ttttttattt actgtgatct gctttccaga tatttttcga
      481 tcaaacaact aatgcgccaa acatttattg cgcgtaaaat atcgtttatt tcattaatac
      541 atttcaggga tgaatatatg tcaccgcaga ataatcatct gcagcgtccg cctgctgctg
      601 tgttatacgc cgatgaactg gcaaaattaa aacaaaatga taacgcacct tgcccgcccg
      661 gttggcagtt aagtttgcct gcggcccgtg cttttatcct tggcgatagc gcgcaaaata
      721 ttagccgtaa agtggtgatt agtccctccg ctgtcgaacg tatgttagtc actcttgcca
      781 ccggacgcgg tttgatgttg gtgggggaac ctggcaccgc aaaatctctc ctgtctgaat
      841 tactggcaac cgctattagc ggcgatgccg ggttaaccat ccagggcggc gcatctacta
      901 ccgaagatca aatcaagtac ggctggaact acgccctgct tatcaagcat ggtccatcaa
      961 cggaagcgct ggtccctgcc ccgctttacc agggaatgcg cgacggcaaa atcgtccgtt
     1021 ttgaagagat aacgcgtaca ccgctggaag tgcaagactg tctgttaggt atgctttctg
     1081 acagggtgat gacggtgccg gaactcactg gtgaagccag tcagctctat gcgcgagaag
     1141 gctttaatat tattgccact gccaataccc gcgatcgcgg cgtcaatgaa atgagtgctg
     1201 cgctaaagcg tcgcttcgat tttgaaactg tctttccgat tatggatttc gcccaggagc
     1261 tggaactggt cgccagtgct tcggcgcgtt tgctggctca tagcggtatt ccgcataaag
     1321 tgcctgacgc ggtactggag ttactggtcc gcaccttccg cgatctgcgt gccaacggcg
     1381 aaaagaaaac gtcaatggat acactaacag cgattatgtc caccgctgaa gccgtgaacg
     1441 tggcccacgc cgtaggcgtc cgcgcctggt ttttagcgaa tcgcgcaggt gagccagccg
     1501 acctggtgga ctgcattgcc ggaaccatcg tcaaagataa cgaagaagat cgcgctcgtc
     1561 tgcgccgtta cttcgaacaa cgagtcgcca cacataaaga agctcactgg caggcttatt
     1621 atcaagcccg ccaccgcctg ccgtgaggaa agatgcatga gcgagccgtt aattgtcggc
     1681 atccggcatc atagtccggc ctgcgcccgg ctggtgaaat cgttaatcga aagccagcgg
     1741 ccacgatacg tattgattga aggcccggct gattttaatg accgggtaga cgaactgttt
     1801 ttagcccacc agcttccggt agctatttac agttattgcc agtatcagga cggtgcagcc
     1861 cccgggcgtg gtgcctggac gccatttgct gaattttcgc cggagtggca ggcgctacaa
     1921 gccgcacgtc gtattcaggc acaaacttac ttcatcgatt tgccttgctg ggcgcagagt
     1981 gaagaagtgg acgattcgcc tgatacgcaa gaggaaagcc aggccttgct gctgcgtgcc
     2041 acccgcatgg ataacagcga caccctgtgg gatcacttgt tcgaagatga aagccagcaa
     2101 attgcattac cctctgcgct ggcgcactat tttgcccaac tgcggggcga tttccccggc
     2161 gatgcactca atcgtcagcg cgaagccttt atggctcgct ggattgcatg ggcggtgcag
     2221 cgaaataatg gcgacgtgtt agtcgtctgc ggtggctggc acgctcctgc actggcaaaa
     2281 atgtggcgcg aatgtccgca ggaaattaac aagccagaat tgccctcgct ggcaaatgcc
     2341 gttacaggtt gttatctcac cccctacagt gaaaagcacc ttgatgtgct ggcaggatac
     2401 ctttccggta tgcctgcccc ggtctggcaa aactggtgct ggcagtgggg cttacagcag
     2461 gccggtgaac aactgctaaa aacggttctc acccgtttgc gccagcacaa cttgcctgct
     2521 tcgatcgcgg atatggctgc cgcacatctg catgcaatgg cactggcaca gttgcgcggt
     2581 catacactac cgttacgcac tgactggctg gatgccatag caggctcgct gattaaagaa
     2641 gccctgaatg caccgttgcc gtggagctat cgcggcgtta ttcatcccga taccgatccg
     2701 attctgctaa cgttgataga cacattagcg ggtgacggat tcggtaaact tgccccttcc
     2761 acgccacaac cgcctctgcc aaaagatgtc acctgcgaac tggaacgtac cgcaatctct
     2821 cttccggcgg agcttacctt aaatcgcttt acccccgatg ggctggcgca aagtcaggtg
     2881 ttacatcggc tggcaatact ggagatccct gggattgtac gccagcaggg aagtacactg
     2941 acacttgcag gcaacggtga agaacactgg aaattaaccc gcccgcttag ccagcatgcg
     3001 gcattgattg aggccgcatg ctttggtgca acacttcagg aagccgcacg ccataaatta
     3061 gaagccgata tgctggacgc gggtggcatc ggcagtatca ccacatgtct tagccaggcg
     3121 gcgttagcgg gtctggcgtc cttcagtcaa caattactgg agcaactcac attattaatc
     3181 gcccaggaaa atcaatttgc cgaaatgggc caggcgctgg aagtgctata tgccttatgg
     3241 cggctggatg aaattagcgg tatgcaaggc gcgcagatat tacaaacgac gttatgcgcg
     3301 gctatcgatc gcacgctgtg gctgtgtgaa tctaacggca gaccggatga aaaggagttt
     3361 cacgctcacc tgcatagctg gcaagcgctt tgccatattc tgcgcgatct acatagcggc
     3421 gttaatttat ccggcgtttc gctttctgcg gcggtagcct tactggagcg acgcagtcag
     3481 gcgattcatg ccccggcgct ggatcgcggc gcggctcttg gcgcactaat gcgtctggaa
     3541 catcccaacg ccagtgccga agcggcgctg acgatgctgg cgcagttatc cccggcacaa
     3601 tccggcgagg cgctgcacgg tttgctggca ttagcccgtc atcaactggc ctgtcaacct
     3661 gcatttatcg ccggtttcag cagccatttg aatcaactga gtgatgccaa ttttatcaat
     3721 gccctgcccg atttacgcgc ggtgatggcc tggctaccgc cacgagaacg cgggacgctg
     3781 gcgcatcagg tgcttgagca ttatcaactg gcgcaacttc ccgtttcggc actgcaaatg
     3841 ccgttgcatt gtccaccgca agccattgca catcatcaac aactcgaaca gcaggcactg
     3901 gcatcgctgc aacactgggg agttttccat gtctgaactg aacgatcttc tgaccacccg
     3961 tgagctacaa cgctggcgat taattcttgg cgaagcggca gaaacgacgc tttgtgggct
     4021 ggatgacaac gcccggcaga tagatcacgc gctggagtgg ctgtatgggc gcgatcctga
     4081 acggctccag cgtggtgaac gctctggtgg attaggtggc tcaaatctca ccactcctga
     4141 gtggttcaac agtatccaca cgctgtttcc gcaacaggtg attgagcggc tggaaagcga
     4201 tgccgtactg cgctacggca ttgaagatgt ggtgacaaat ctcgacgtgc tggaacgtat
     4261 gcagccttcc gaaagcctgc tacgcgccgt tttgcacacc aaacatctga tgaaccccga
     4321 agtactggct gccgcccgca ggattgtgcg ccaggttgtt gaagaaatta tggcgcgact
     4381 ggcaaaggaa gttcgtcagg ctttttctgg tgtccgcgat cgccgtcgcc gctcatttat
     4441 tccactggcg cgaaactttg atttcaaaag tactctgcgc gccaacctgc aacactggca
     4501 cccgcaacac ggcaagttgt atatcgaatc cccccgcttt aacagccgca ttaaacgcca
     4561 aagcgaacaa tggcaactgg tcttactggt tgatcaaagc ggctcgatgg tcgattcggt
     4621 gatcccctct gcggtgatgg cggcctgttt gtggcagtta cccggcattc gtacccatct
     4681 ggtggcgttt gacacaagcg tcgttgatct cacggcagac gttgccgatc cggtagagtt
     4741 attaatgaaa gtacagttgg gcggcgggac caatatcgcc agtgccgtgg agtatggtcg
     4801 gcaacttatt gaacaaccag cgaaaagcgt cattatcctc gtgagcgatt tttacgaagg
     4861 gggttcatca tcattactga cgcatcaggt gaaaaagtgt gtccagagcg gcatcaaagt
     4921 gctgggactg gcagcgctcg atagcacagc aacaccttgc tatgaccgcg atacggccca
     4981 ggcgctggtt aatgtcggcg cacaaatagc cgccatgaca ccgggcgagc tggcatcatg
     5041 gcttgcggag aatcttcagt catgaattca ctacgtccgg aattattaga actgacaccg
     5101 caggccctga cggcgttaag caatgccggt tttgttaagc gcagtcttaa ggaactggaa
     5161 aatggcaacg tcccggagat cagccatgag aacggcgctt taatcgccac cttcagtgac
     5221 ggtgtccgta cccagctggc gaacggccag gcactgaaag aggctcagtg cagctgcggg
     5281 gccagcggta tgtgccgtca tcgcgtgatg ctggtgttaa gttatcaacg actttgtgct
     5341 accgcccagc ctacgggaaa agaagaggaa tgggatccgg caatctggct ggaagaactg
     5401 gctacccttc ccgatgccac ccgcaaacgc gcacaggcgc tggtcgctaa aggcatcacc
     5461 attgagttgt tctgtgcgcc gggtgaaatt ccctctgccc gcttaccgat gagcgatgtg
     5521 cgtttttatt cccgcagcag tattcgtttc gcccgttgtg attgtattga aggcacactt
     5581 tgcgaacatg tcgtactggc agtacaggcc tttgtccagg caaaagcgca gcaagcggaa
     5641 ttgactcatt taatctggca gatgcgcagc gaacacgtca catcatctga cgatccgttt
     5701 gccagcgaag aaggcaaaac gtgtcgtcaa tatgttcaac aactaagcca ggcattatgg
     5761 ctgagcggca tcagccagcc gcttattcac tacgaggccg ctttcagccg cgcgcagcaa
     5821 gcagcggaac gctgcagctg gcgatgggtg agtgaatcgc tacggcaact gcgcgccagc
     5881 gttgatgcct tccacgcccg cgccagccac tatcatgccg gagaatgctt acgtcagctt
     5941 gcggcattaa acagtcgatt aaactgcgca caagagatgg cccggcgcga cagtgttggt
     6001 gaagttcctc ctgtgccgtg gcgcacggtc gttggctctg gcattgccgg agaagcaaag
     6061 cttgatcatc tgcggctggt gtctttaggt atgcgttgct ggcaggatat tgagcattat
     6121 ggtttacgca tctggtttac cgatcccgac accggcagta ttttgcatct ttcgcgtagt
     6181 tggccgcgaa gtgaacagga aaactcaccg gcagctacgc gtcggctgtt tagttttcag
     6241 gctggcgcac tggcgggtgg gcaaattgtt tcacaagcag caaaacgcag tgccgatggc
     6301 gagctgctgt tagctacccg caaccgctta agcagcgttg tgccgctgtc gccagacgcc
     6361 tggcaaatgt tgagcgcgcc gttacgccag ccgggcattg tggctttgcg ggaatattta
     6421 cgccagcgtc cccccgcctg catacggcct cttaatcagg tggataactt atttattctg
     6481 ccggtcgctg agtgtatttc actcggttgg gacagcggcc gccagacgct ggatgcgcag
     6541 gtaatcagcg gcgaaggtga agataatctg ctgacgttat cccttccggc atctgcctgt
     6601 tctccttttg ccgttgaacg catggcggcg cttttgcaac aaacagacga ccccgtgagt
     6661 ctggtttctg gctttgtcag ctttgtagag gggcaattga cactggaacc acgggtgatg
     6721 atgacaaaaa cccgtgcctg ggcgctggac gcggaaactg cgcctgtggc accgctacct
     6781 tctgccagcg ttttgcctgt gccgtctacc gctcatcagt tgctgatgcg ctgccaggcg
     6841 ttacttattc aactgctcca taacggctgg cgctatcagg aacagagcgc tattagtcag
     6901 gcagagttgc tggcgaatga cctcaccgcg gtgggttttt atcggctggc acatgtgttg
     6961 gtacaatttc gtaatacaga aagcgaggca cgggtagaag caatgaataa cggtgttttg
     7021 ctttgcgaac aattattccc catgcttcag caacaaggat gaaatagtgc tttttactaa
     7081 gagttctact ccagttccgg actgctcacg ccacggtatt aggcatatcc tatatagccc
     7141 ctggtgagag tcaccagttt cttgattaaa taaaatggag ttttacatga aggctttcaa
     7201 taagctgttt tccctcgttg ttgcatctgt tctggttttc tctcttgctg gctgcggtga
     7261 caaagaagaa tcgaagaaat tcagcgccaa tctgaacggc actgaaattg ccattaccta
     7321 tgtctacaaa ggtgacaagg tgcttaagca atcttctgaa accaaaattc aatttgcctc
     7381 cattagtgca accaccaaag aagatgctgc caagacactt gagccgttaa gcgccaaata
     7441 caaaaacatc gcgggtgttg aagaaaaatt aacctatacc gatacctacg cgcaggaaaa
     7501 cgtgactatc gatatggaaa aagtggattt taaagccctg cagggtattt caggaatcaa
     7561 cgtttctgct gaagatgcca aaaaaggtat tactatggcg caaatggaac tggtgatgaa
     7621 agccgctggt tttaaagaag tgaaataatc tgtcggcggc catgtttcgt atggccgcca
     7681 tacccgtctt agcttttctt cacatgctgg cgcgctgcca gtccacgcag aaaataacgt
     7741 aaaaattgat cgccgcattc gcggaagttt ttatgatccg gtgcgcgcat catcgctgta
     7801 atttccggca tcgaaacgcg gaactgctgt tcggtgagga tagccagaat gtcatcggtt
     7861 ttcagcgaaa acgcgatgcg taattttttc agcacgatgt tgttattaat gcgacgttcc
     7921 ggctccagtg ccggagcaga ctcatccttg ccgcgttttt cataaatcag gccattgagg
     7981 aatgacgaca aaacaatgtc cggacaacgc tgaaaaccct cttcgtcttc tttacgtagc
     8041 cagacggcga tctgttccgc ggtggcttcg acattaccca gcgccagaat acgcaccagg
     8101 tcattattat tggctttcaa aatgtagcgc acgctgcgca gaatatcgtt acttagcatg
     8161 aggccttcag gtgttgatga ggcaaaaagc cattttagca gtcttttaca ggccaatcgc
     8221 ctcttttaag cttttcagat aacggcggct gaccggcacg gttaagccat tacgcaaaat
     8281 caactcggcc tggccgttat cttccagacg aatctcctgt aaatgcgcga ggttaaccag
     8341 atactgacga tggcagcgca gtagtggtgt acgactttcc agggtacgta atgtcaattc
     8401 ggtaaagccc tctttccctt cgtggctggt aacgtagaca ccgctcatcc gactgctgac
     8461 aaatgccaca tctttcattt gcagcaaata aatccgacta tgccccgtac aagggataaa
     8521 tttcagcgcc tgttgatttt ccggtaacag cgaaacatcc tgcttgctgc gctcctgacg
     8581 caatcgcgcc agcgttttct ccagtcgcgc ttcatcaatt ggcttcagca gataatcaaa
     8641 ggcatgttct tcaaaggctt taatagcgta ttcgtcaaac gcagtgagaa aaacaatata
     8701 cgggcgatgt tccgggtcaa gcatccccac catttccaga ccactgatgc gcggcatctg
     8761 gatatcgaga aacagcacat ccgggcgcag tttatgcacc gcgccgatcc cttccacggc
     8821 gtttgaacac tctccaacga tttcaatatc gctctgctcc tgcaaaaata cacgcaggtt
     8881 ctcccgtgct aacggttcat catcgacaat taagacttta atcatgcctc gtccctccat
     8941 ggtagttgta acgttattcg ggtgtaacta tcaggctcac aggcgacgct tattccatag
     9001 tcatcgccaa accgttcacg taaacgctta tccaccagat tcatccccag cccactggca
     9061 ttggttaccg gttgatacaa accggcattg tcttcgatct ccagcatcaa atgttgcccc
     9121 tcacgtcggg cgctgattgc cactcgccct gtatccagca gttgtgatgt cccatgttta
     9181 atggcgtttt ccactatcgg ttgcagggta aacgcgggca attgctgctg ggataattct
     9241 tgcggaatag caatgttgac ctgcaaccgc gactggaagc gcgccttttc aatttgcaga
     9301 taagcgttca catgttcaat ttcgtcggcg agagtaacaa actccgaagg ccgctttaag
     9361 tttttgcgga aaaaagtgga aagatactgc accagctggc tggcctgttc gctgtcgcgg
     9421 cggatcaccg ctttaatggt gttaagcgca ttaaacaaaa aatgaggatt cacctgggcg
     9481 tgaagcagtt tgatttctga ctgggtgagc atcgcttttt gccgctcata ttgcccggca
     9541 agaatctgcg ccgaaagcag ttgcgcaatc ccctcgccca gcgtgcggtt tattgaactg
     9601 aataaacggt ttttggcttc atacaatttg atggtgccca tcacccgctg attttcacca
     9661 cgcaacggaa ttaccagcgt cgaccccagt ttgcattgcg gatgcaaaga gcaacgataa
     9721 ggtacttcgt tgccatcagc gtagaccact tcaccggttt caatcgcttt taaggtgtaa
     9781 gtcgaagaaa tcggtttgcc gggtaaatgg tggtcgtcac caattccagt aaaggccagc
     9841 aatttctctc gatcggtaat cgcgactgca ccaatatcca gctcctgata cagcacctgt
     9901 gccactttca tgctgttcac ttcgttaaac ccctggcgca aaatgccttc cgtcgaggca
     9961 gccactttca gcgcagtggc agaaaaagcc gaagtgtatt tttcaaacat cgcgcgttta
    10021 tcgagcaata tacgcataaa cagcgccgcg ccgacggtat tggtgaccat cattggtgca
    10081 gcaatattac tcaccagacg caccgcatct tcataaggtc gggcgatcgc caggatgatc
    10141 aacatttgca ccatttcagc gacgaacgtg acggcaccgg cggtaatggg gttaaagact
    10201 ttatcagtac gcccgcgacg aatcaggatg ctgtgtacca ggccgccgag caatccttca
    10261 acgatggtcg agatcatgca acttagcgcg gtcatgcccc ccatcgaata tcgatgtaac
    10321 ccgccggtca ggccaaccag cccaccgacg accggaccgc cgagtaagcc gcccattacc
    10381 gcgcctatcg cacgggtatt ggcaatagaa tcgtcaatgt gcaacccaaa ccaggtgccc
    10441 atgatgcaga agatggaaaa gacgatgtag cagagaaatt tatgcggcag acgaaccgtg
    10501 acctgcatta acggtatgaa taatggcgtt ttactcatta accacgcaat gactaaaaaa
    10561 acgcacatct gctgaagcag cagcaacacc agattaaaat cgtacatacc cgcaaaccac
    10621 acttcccttt aaaacgcgta acatacattg cctgcgttta actttctttg aactcttgca
    10681 gaaaaatgag aattcgtgag tacgatcact caaaatcgcc tggcaaaaat aaaatcaccc
    10741 tatagatgca caaaaaacgg gcaaaactac ctggttcgca aaactgcgtc taaagttaaa
    10801 ccgggacctc gcgagcaagg gtgagacgat ggcgctttac acaattggtg aagtggcgtt
    10861 gctttgtgat attaaccctg tcacgttacg cgcgtgacgc aacattttaa aaaatcgacg
    10921 aagatcgtcc aggagcgccg tttttcaagg gttggataga tgattcgttt accatgtcga
    10981 cgaacttcga cgaatttcgc cgataaaaag tgtcccatac atgccccatt gatgccccat
    11041 aaacaatatc agaaattgat actggttatc catacagata aaaataacac ctgtacataa
    11101 ctacagttac aggagggtgt tatgcgtgtt gagatctgta tcgctaaaga aaaaatcact
    11161 aaaatgccaa acggtgctgt ggatgcgtta aaggaagaat taacccgacg catcagtaaa
    11221 cgttatgacg atgtagaggt gatcgtaaaa gccaccagca acgatggcct ttctgttaca
    11281 cgcaccgcag ataaggattc tgcaaaaaca tttgttcagg agactctgaa agatacctgg
    11341 gagtctgctg acgagtggtt tgttcactaa taaacacgta aaatcggtaa cggctggaaa
    11401 tcattcaata ctcgcactat cgaaagttta ccagccaacc gcagcacgtc ctgcatacgt
    11461 cgtgtctgcg gtttttcttt ttcgcttaca ttgtgtctgg ttcttccggc cactcaatat
    11521 caggtgcagt tgatgtatca acacagttca gcaacacccg atacttcttc caggcttcca
    11581 gcaacgagtt ttcttcctcc gttgcgatct ccagatctac agcatcctga agtggcgcaa
    11641 tatgctcact gaattcctgg atgtagaact gtgtggtgac ggtcttccag ccattcggct
    11701 cctgctgtat cgaagcatac caggctattt caatatcgct atgctgcagc agcatttaac
    11761 cccttgtaat tcatcgccat aattgattta attcacaaat aaaactataa tatggtgaaa
    11821 ttaatgaaaa aaaacacaga tgatggggct aaaatttaca caccaattac cctaaagctt
    11881 tatgactggt gggttttggg agtatcaaat cggcttgcat ggggatgtcc tacaaaggaa
    11941 caccttcttc cacactttct ggaacattta ggtaacaacc atctggatat tggtgttgga
    12001 actgggtttt accttactca cgtacctgag agtagtctga tatctttaat ggatttgaac
    12061 gaagctagcc tgaacgcggc atctacaagg gctggggaat caaaaattaa acataaaatt
    12121 agccatgatg tttttgaaac ttatcccgcg gcgttacatg gtcaatttga ttccatttcc
    12181 atgttttacc ttcttcactg cctgcctgga aatatatcta caaaaagctg tgtaatacgc
    12241 aatgcggcgc aggccttaac tgatgatgga actctatacg gagccacaat tcttggcgat
    12301 ggagttgtgc acaatagctt cggtcaaaaa ctgatgcgca tttacaatca gaaaggcatc
    12361 ttttcaaaca caaaagattc cgaagaaggc ttaacacata tactctcaga gcatttcgag
    12421 aatgttaaaa ccaaggttca aggtactgta gtaatgtttt ctgcttcagg gaaaaaatag
    12481 catccaaccg cagcacgttc ttgcttaaga cgtgctacgg tttcctttac gatttggggt
    12541 tgcgacttta cctctattga taatgcattc cggcagaaca ttcaaatatg aatgtacgat
    12601 atttaactaa ccgaaaaaca aaatatagca caggcgagac atttaccatc taagaaaata
    12661 gtatcgtttt tttacagtta aatcaacatc acttccttaa aatgaaaaca ataaatttca
    12721 atcctgaaac attttatttt agcttattat ttcccagtat cacattttat caggatatcc
    12781 ttctgcgagg ttattatgct tcctgtaaat aatccccccc tatccactgg aaacgtctct
    12841 ttttacagaa ctacatcaat cgacaatgtt cacaataatt atctctccga atgggttgaa
    12901 tggactaaaa acagcatttc cggagaaaac agggaaactg cttttacccg gctccaatta
    12961 tgtctggaaa acagtgaaac atcgttggac ttatcttgtt taggtctcag atctctacca
    13021 cgattgcctg acaatcttga tgaaattaat gtaagcaata accaactatc aatgctcccc
    13081 gagctaccaa gggcattgaa agaactgaat gcaagcagta atcaattatc tgcacttcct
    13141 gaattaccag tgtcgctgga atatataaat gtgagtgata accatttgtt cgcacttcct
    13201 gaattacctg cgtcactaga atatattaac gtaagtgaca atcacctgtc tgtacttccg
    13261 aggttaccaa tgtcattgga attacttgat gcagccagaa atgctttgga agtaatacca
    13321 gattttccag aaagagatga tcatattata agaatattct ggcttaatca gaaccggatc
    13381 acggcaattc cggaaagcat acttggcctc agttctgata gcgttgtcaa tcttagagaa
    13441 aatcaactat ctcccagaat aatgcaaact ttgttacaac aaaccgccca
//
LOCUS       DADLFA010000109        13479 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000109 DADLFA010000000
VERSION     DADLFA010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13479
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.109"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(102..3323)
                     /gene="carB"
                     /locus_tag="JQH65_002675"
     CDS             complement(102..3323)
                     /gene="carB"
                     /locus_tag="JQH65_002675"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAY6140046.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(3341..4489)
                     /locus_tag="JQH65_002676"
     CDS             complement(3341..4489)
                     /locus_tag="JQH65_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase small subunit"
                     /protein_id="HAY6140047.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            complement(4944..5765)
                     /gene="dapB"
                     /locus_tag="JQH65_002677"
     CDS             complement(4944..5765)
                     /gene="dapB"
                     /locus_tag="JQH65_002677"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAY6140048.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSRESHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNSL"
     gene            complement(5932..6846)
                     /gene="rihC"
                     /locus_tag="JQH65_002678"
     CDS             complement(5932..6846)
                     /gene="rihC"
                     /locus_tag="JQH65_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HAY6140049.1"
                     /translation="MHLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMSGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPELFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            complement(6912..7862)
                     /gene="ispH"
                     /locus_tag="JQH65_002679"
                     /gene_synonym="lytB"
     CDS             complement(6912..7862)
                     /gene="ispH"
                     /locus_tag="JQH65_002679"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HAY6140050.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(7864..8313)
                     /gene="fkpB"
                     /locus_tag="JQH65_002680"
     CDS             complement(7864..8313)
                     /gene="fkpB"
                     /locus_tag="JQH65_002680"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAY6140051.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(8382..8876)
                     /gene="lspA"
                     /locus_tag="JQH65_002681"
     CDS             complement(8382..8876)
                     /gene="lspA"
                     /locus_tag="JQH65_002681"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HAY6140052.1"
                     /translation="MSQSICSTGLRWLWLMVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSR
                     AKKQ"
     gene            complement(8876..11692)
                     /gene="ileS"
                     /locus_tag="JQH65_002682"
     CDS             complement(8876..11692)
                     /gene="ileS"
                     /locus_tag="JQH65_002682"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAY6140053.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHFSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYHDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(11735..12676)
                     /gene="ribF"
                     /locus_tag="JQH65_002683"
     CDS             complement(11735..12676)
                     /gene="ribF"
                     /locus_tag="JQH65_002683"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HAY6140054.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYVVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(12684..12902)
                     /locus_tag="JQH65_002684"
     CDS             complement(12684..12902)
                     /locus_tag="JQH65_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 domain-containing protein"
                     /protein_id="HAY6140055.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            13005..13268
                     /gene="rpsT"
                     /locus_tag="JQH65_002685"
     CDS             13005..13268
                     /gene="rpsT"
                     /locus_tag="JQH65_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAY6140056.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT         3149 a         3804 c         3399 g         3127 t
ORIGIN      
        1 aaaagattta cttaatgggc aaaaaaaacg gtcctcatca gagaaccgta ttacttattc
       61 gatcaaatta gcggatgaaa aatatccgcc atgacacgct attatttgat ctgcgcgtgc
      121 atttcctgca ccgaaattac tttttcggtc gcgtcggcat tcagcgccat cgcggtagcg
      181 aaaccaccgt tcagggtggt gtcataatgc actttatatt gcagcgcact gcgacggatc
      241 acgcgggagt cttcaatcgc gcgacggcct gaagtggtgt tgatgatgta ggtatattcg
      301 ccattcttga tgcggtcctg aatgtgcgga cggccttcat gcaccttgtt caccagacgc
      361 ggattaatgc ccgcttcgcc cagcacaatc gccgtgccgt gggtcgcatc cagctcgaag
      421 ccctgtttca gcagttttgc cgccaggtcc accacgcgtt ctttatcgcc ttcgcgcacg
      481 gaaagcaacg cacgaccgtg tttcttcatg gtggagttgc tgcccagctg cgctttggca
      541 aacgcttcag cgaaggtgcg gcccacgccc atcacttccc cggtggagcg catttctggc
      601 cctaacagcg ggtcaacacc cgggaattta ttgaacggca gcaccacttc tttcaccgag
      661 tagtacggcg ggataacttc tttggttacg ccctgctcag ccagcgattt gccagccatc
      721 acacgcgccg ccacttttgc cagcggtacg ccggtggctt tggagacgaa cggaacggta
      781 cgcgccgcac gcgggttaac ttcaatcagg tagacttcgt tgtttttcac cgcaaactgc
      841 acgttcatca gaccgcgcac ctgcaattcg aaggccagtt tctgcacctg ctggcgcatc
      901 acatcctgaa tttcctgact taaggtgtag gctggcagag aacatgcgga gtcaccggag
      961 tgcacgcccg cctgctcgat gtgctccatg atgccgccaa tcagcaccat ttcgccgtcg
     1021 cagatggcat ccacgtcaac ttctaccgcg tcatcaagga agtgatccag caacactggc
     1081 gcatcgttag acacgctgac cgccgtctgg aagtagcgac gcaggtcagc ttcgtcatag
     1141 acgatttcca tcgcccgacc gccgagaacg taagacggac gtaccaccag cgggtagcca
     1201 atctctttcg ccttctctac cgccatttca atagcggtaa cggtggcgtt cgccggttgt
     1261 ttcagtttca gacgctcaac cgcatgctgg aagcgttcac ggtcttctgc acggtcgata
     1321 gcatccgggc tggtgccgat aaccggtacg ccagcagctt ccagcgcgcg cgccagtttc
     1381 agcggggtct gaccgccgta ctgaacgata acgcctttcg gcttctcaat acgcacgatt
     1441 tccagcacat cttccagagt taccggctcg aagtagaggc gatcggaagt gtcgtagtcg
     1501 gtggagacgg tttccgggtt acagttaacc ataatggttt cgtaaccgtc ttcgcgcagc
     1561 gccagcgagg cgtgtacaca gcagtagtcg aattcgatac cctgaccgat acggttcggg
     1621 ccgccgccga gaaccatgat tttttcacgg tcggtagacg gattcgcttc gcactcttct
     1681 tcataagtgg agtacatgta agcggtgtcg gtggcgaact ctgccgcaca ggtatccacg
     1741 cgcttataaa ccgggtgcag gtcatactgg tcgcgcagct tgcggatttc cgcttcgcgc
     1801 acgcccgcca gttttgccag acgcgcatcg gcaaagcctt tgcgtttcag ctggcgcagg
     1861 aattcagcgt tcaggccagt gatgcccact tccgctactt tctcttccag acgcaccagt
     1921 tcttcaatct gtaccaggaa ccagcggtca atattggtca ggttgaacac gccgtccaca
     1981 gacaggcccg cacggaaggc atcggcgatg taccagatac gctctgcgcc agcatctttc
     2041 agttcgcgac ggattttggt taacgcttcc gggtcatcga ggctcacttt cgggtcgaat
     2101 ccagtcgcac cgacttccag gccgcgcagc gctttttgca gggattcctg ctgcgtgcga
     2161 ccaatcgcca tcacttcgcc aaccgatttc atctgagtgg tcagacggtc gttagcacca
     2221 gcgaattttt cgaagttgaa gcgaggaatt ttagtaacca catagtcgat ggacggctcg
     2281 aaggaggccg gagtacgtcc gccagtgatg tcgttcatca gttcgtcgag ggtgtaaccc
     2341 accgccagtt tcgccgccac tttagcaatc gggaaaccgg tcgctttcga cgccagcgcc
     2401 gaagaacggg acacgcgtgg gttcatttcg ataacaatca ggcgaccgtt tttcgggttc
     2461 accgcaaact ggacattgga accaccggtt tcaacgccga tttcacgcag caccgccatc
     2521 gaggcgttac gcatgatttg atattctttg tcggtcagcg tttgggctgg cgcgacagtg
     2581 atggagtcac cggtatggat gcccatcgca tcaaagtttt cgatagagca gacgatgatg
     2641 cagttgtcgt ttttatcacg caccacttcc atctcgtact ctttccagcc gatcagcgac
     2701 tcatcaatca gcagctcttt ggttggggag agatccagac cgcgggcgca aatttcttca
     2761 aactcttcgc ggttataagc gataccgccg ccgctaccgc ccatggtaaa ggatgggcga
     2821 ataatgcacg ggaagcccac gtcagcggca accgccagcg cttcttccat cgtgtgtgcg
     2881 ataccggaac gcgcggtttc cagaccaatt ttcttcatcg ctacgtcgaa acgacggcgg
     2941 tcttctgctt tatcaatcgc atcggcggtc gcaccaatca tagtcacgcc gaactcttcc
     3001 aacacgccct gacgctccag ctccagagcg cagttcagcg ccgtctgacc gcccatcgtt
     3061 ggcagcaccg cgtccgggcg ctctttttca ataatcttgc gtactacttc ccagtgaatc
     3121 ggctcgatgt aggtcgcatc ggccatttcc gggtcggtca tgatggtcgc cgggttggag
     3181 ttcaccagaa tgacgcggta accctcttcg cgcagggctt tacatgcttg cgcgccagag
     3241 tagtcaaact cacacgcctg accgataaca atcgggcccg cacccagaat caggatactt
     3301 tttatatctg tacgttttgg catggctctt ttactcctga ttacttagcg gttttacggt
     3361 actgctcaat taactcgata aagtggtcga acaacggcgc tgcgtcgtgt ggcccagggc
     3421 tggcttccgg gtgaccctgg aagctgaacg ccggtttatc agtgcgatga atgccctgta
     3481 acgtaccgtc gaacagggat ttatgcgtga cacgcaggtt tgcaggtaat gttgcttcat
     3541 ccaccgcaaa accgtggttc tgggcggtga tcatcaccac gtttttctcc acatctttaa
     3601 ccggatggtt gccgccgtgg tgaccaaatt tcattttgac agtcttcgca ccgctcgcca
     3661 gcgccagcag ctgatgaccg aggcagatgc cgaataccgg aatatcggtt tcgaggaatt
     3721 tctggatggc ggtaatggcg taatcgcacg gggccgggtc gccaggaccg ttggagagga
     3781 agatgccgtc tggattcatt ttcagcacat cttccgcaga agtttgcgcc ggaacgatgg
     3841 tcaggcgaca gcctctatcc accagcatcc gcaggatgtt gcgcttggca ccaaaatcat
     3901 aagccacgac gtggaacggc agctcgtctt cttttttcgc ttctggcagg ccgccggtca
     3961 acgtccagct cccttgtgtc cagctatagg cttctgcggt ggtcacttct tttgccagat
     4021 ccatgccatt cagacctggg aacgcgcggg ctttttctaa cgccaacgcc gcatccgggt
     4081 tatcgcctgc gataatgcag ccattctgtg cgcctttctc gcgcagtaaa cgcgtcagct
     4141 tacgggtatc gatatcggca atcgccacga tgttatggcg cttcaggtaa gaagagaggt
     4201 cttcggtatt acggaagttg ctggcaatca gcggcaggtc gcgaatcacc agaccttgtg
     4261 catgtacctg agaagattct tcatcggcgt cattagtgcc gacattgcca atatggggat
     4321 aagtaagagt aacgatttga cgagaatagg aaggatcagt gaggatttct tgataaccgg
     4381 tcattgaagt attgaaaacg acttccccaa ccgccgaacc tgttgcccct atggcccgac
     4441 cgtgaaactg ggttccgtct tccagaacca atagcgctga cttaatcaaa acaccctcca
     4501 gagaatattc actcacttta tttgcatatt aattcacaat gatgacgtaa atcaatgcaa
     4561 atctgcttac cgacgaattt ctggcaaacg gcggcattct ggagatatgg gcgctaaaag
     4621 tcaacttaat ggcgggattt tttatattat tttgtggctc atgcatgttt tacaggcatt
     4681 aagcgacaaa aagatcatat aacctgttct ggaaaacgct tgcacagcaa gaagattgca
     4741 tgaaataaaa gggagcaggg aaaagtggac caaatggtca aattttacat taaaacaaca
     4801 aaaacagaag acaaaacatg taatttttag cacttataga caatttaaaa acataacact
     4861 aaaaataaaa gggccaaaat taaagccctt tataatcaat aaattaaatg tgttattttt
     4921 gcaccataac aaatattttg tggttacaaa ctattgagat caagcacatc tcgcatatca
     4981 aaaagaccac tttccttacc actcaaccac aaagccgatc ttaccgcgcc gttagcaaat
     5041 gtcatgcggc tggacgcctt atgggtgatc tccagacgct cgccaatatc ggcaaacatc
     5101 gcggtatgtt cgccaacgat gtcacctgca cgcacggtgg caaaaccaat ggtgccaggc
     5161 acacgttcac cggtgtggct ttcacgactg tagaccgcgc aatctttcag atctttatca
     5221 agggcgtggg cgatcgcctc tcccattgcc agtgcggtgc ctgacggcgc atcaacttta
     5281 tgtctatgat gtgcttcaat aatttcgata tcggtgtagt cacccatcac tttggctgct
     5341 ttctccagca gcttaagcat gacgttaacg ccaacgctaa aattggcggc aaagacaatc
     5401 gcaatatcgg cggcagcgtc acgaatcgcc tgtttaccgg cttcgtcaaa ccccgtagtg
     5461 ccgatcacca tccctttgcc atgctggcga caaaaagcga gatggttcag cgtaccttcc
     5521 ggacgggtaa aatcgataaa cacatcaaaa tcatctttta ccgcatcgag gctgctttgc
     5581 acggtaacgc ctgttttccc tgcaccagcc agctcaccgg cgtcgctgcc cagtaaagaa
     5641 gatccttcgc gctccagcgc cgcgcccagc tgcacgcctt ctaatgccag cgccgcctga
     5701 atcaactggc ggcccatacg ccccccggct cccgcgatgg caacgcggat gtttgcatca
     5761 tgcatagcta ttctcttttg ttaatttgca tagaccgttt tcagagtaac cagcgcatcg
     5821 ctacgccgcc agccaaaaac gctgataatt agaaattaat gataaacagg gagggatatc
     5881 agtgaaaggc atgagtcgat gaatgactgc atgccgataa catgtgacag gttacgacgc
     5941 cagagccagc acctcagcca cccactgctg gaagcctttc acatccagat ccaatgccac
     6001 ctttacattg gctggtttgc ccaggcaacc gtcgatatca accaccgtcg tgcctgaggt
     6061 aaattcgccc tgagtttcca ctgccacaaa acacggtttg agagtgaaca attccgggcg
     6121 caccagccag gcaatagcgc aaagatcgtg catacgcagt ccactttgca tactgccgct
     6181 acggtagtgg ctaaacaggg cgtggagcat ttttccggtg cgattcaact ccggcaaagt
     6241 agcgagatag tcaggggtta atattgcctg attggtgaca tccagaccgc tcatgacgat
     6301 ttcgatgccg ctacggaaga cacaggcagc cgcttctgga tcggcagcga tattaaactc
     6361 ggcgtttggt gtgcagttgc cgcgaccagc agaaccgccc atgatcacca gacgacgaat
     6421 atacggttta cattccgggc attgtgaaag taacagcgca atattggtta acgggccgat
     6481 ggccaccagg gtaacgggct ctggtgcacg catcagggca tcccgaatcg ccagaaacgc
     6541 tggtatcccg agcggctttc ggttgtgctc aacaaagtcg tagccagcca ttcccgattc
     6601 gccgtgcaca gatgccgcat cacgcggtgc gcgtaccagc ggcacagcgg ctccctgggc
     6661 gagcggaatc tccgcattcc agaaatgcag cagttgcagg gcgttgcggg tggttttctc
     6721 aaccgagaca ttacccgcga cggtggtcat cagttgcagg tcgagttcgg gtgcaaaaat
     6781 cgcggcggca atggcgacgg catcgtcaat gccggggtca gtatcgagga agataggtaa
     6841 atgcatgttt tctccataaa aaatgccggt aacaagaccg gcattttcgc ataacttagg
     6901 ctgctaatga cttaatcgac ttcacgaata tcgacacgca gctctttcgg cacttcgaaa
     6961 acaatgtttt cttcacggcc ttccagcgga atggcttcac caccgcccag ctgctgcaaa
     7021 cgtgccacca cattctgcac cagaatatcc ggagccgatg cgcccgcagt cacgccgacg
     7081 catttaacct ctttcaccca ctcttcctgg atgtctttcg catcgtcaat caaaaacgcg
     7141 cgtttgccca tacgctgggc cagctccgcc agacggttgg agttggagga gtttttcgaa
     7201 ccgaccacca acacaacttc cgcctgttct gccagggcgc gtaccgcttc ctgacggtta
     7261 gtcgtggcgt agcagatgtc atctttgcgc ggaccgacaa ttttcgggaa gcgtttacgc
     7321 agcgcgtcga tcacatcaga cgtgtcatcc accgacagcg tggtctgggt cataaaggag
     7381 agcttctctt cgtttttgac cgtcagtttc cacacatcgt ccggcgattc gaccagatac
     7441 attccccctt ccgggttact gtactggccc attgtccctt ccacttccgg gtgcccggcg
     7501 tgaccgatga gaatagattc ttcgccacgg cgactggcgc gggcgacttc catatgcact
     7561 ttggtcacca gcggacaggt cgcgtcaaat accgtcaaat cgcgactttt tgcttcgtta
     7621 cgtaccgcct gagaaacacc gtgtgcggag aaaatcagga tcgcgccgtc cggtacttcg
     7681 ctaatctgct caataaagat agccccacgc tcacgcaagc tatcgaccac atagcggtta
     7741 tgtaccactt cgtgacggac atatatcggt gcgccgtaaa tggccagcgc gttttcaaca
     7801 atgctgatag cgcggtctac cccggcacaa aaaccacgcg ggttggccaa caggatctgc
     7861 atgttacgcc tccaatgccg gatcgatttc cagcacttca atatcaaaat gaacggtctg
     7921 cccggccaga ggatggttga aatcaacggt aatggagtcg ccgttaattt cgcggatcac
     7981 gccaggcatc tcactgccat ccattgcggt aaaaagcatg attgcgccaa tttctggctc
     8041 gcctgcatcc ataaattcac ggcgggagaa gtactgaatc aggtccggtg acggcacgcc
     8101 aaacgccgca tctggctcca acgagaaggt ggttttatcg cccactttca gccccaacag
     8161 gtgttgctcc agcccttcag aaagagaagc atcacccagg cggaacagcg ccggtttacc
     8221 gttgttgcgg gtagactcgg cggtggtgcc atcgtcgagt tttagcgtga agtgcaccag
     8281 gacggcgcta ttgctctgta cagattcaga catgcaggtt tgctcttata tttgaggatt
     8341 tttgccggat gcggcgtaaa cgccttatcc ggcagggttt attattgttt tttcgctcta
     8401 gaaggcaaaa aaccttccag cacaatcagt gccgcaccga cacagatggc agtatcggca
     8461 aggttgaagg tggcgaagtg ccagtcgccg acgtagaagt cgatcatatc gacaacgaag
     8521 ccgtgccaca ggcggtcgaa caggttgccc agcgcgccgc caataatcag cgcgtaagcg
     8581 atattgttta gcttctgcgt ggccttcgag cgatacatca tcactgccag gatcacgcta
     8641 ataccaatcg caataccggc aaagaaccaa cgctgccagc cgccgctatc ggcaaggaaa
     8701 ctaaacgccg cgccatagtt acgcgcataa tgcagattaa gcgacgggaa cagcgggacc
     8761 gtatccccca gagcaaagtt ctggaggatc aggtatttgc tgcccagatc gataatcagc
     8821 acgactacca tcagccacag ccagcgtagc cctgttgaac agatcgattg actcatcagg
     8881 caaacttacg tttttcaccg tcaccggcga cgttgctgac acagcggccg cagatttctg
     8941 cgtgttccgc caccttgccg acatcctggg tgtagtgcca gcagcgtggg cacttctcac
     9001 cttcggcttt actcaacgcg actttcagcc ctttgagtac ttcgctctgc tgagcatcag
     9061 caggtgcgtc gtgatagtct gcaacggtag cgccggaggt caacaggaca aatcgtaatt
     9121 catcgcccag cgcggtcagt ttcgccgcca gttccggttc tgcatacaag gttactgccg
     9181 cttccagcga gccacccact ttcttgtcgg cacgcgcttg ctcaatgact ttgttcactt
     9241 cgccacgcac tttcaacagc tcgtcccaga acgcatcgtt catcgcttcg ctgtctgcca
     9301 ggccaaacag gccttcgtac cactcaccgg tgaagacgta tttttcacgt tcgcccggca
     9361 ggtagcccca cacttcatca gcggtgaagg agaggattgg tgccatccag cgcaccagcg
     9421 cttctgcgat gtgatacagc gcagtctggc agctacgacg cgccacactg tccgctttgg
     9481 cggtgtactg acggtctttg atgatgtcga ggtagaagga acccatctca acggagcaga
     9541 agcgcatcag acgctgtacc acttcgtgga aatcgtatgc ttcgtacgcc ttgaggatgt
     9601 cttcctgtgc cgctttcgca caacctacgg cccagcgatc cagtaccacc atctcttccg
     9661 gtttcaccat atcttttgct ggatcaaaac cgttcaggtt tgccagcagg aagcgcgcgg
     9721 tgttacggat acgacgatag ctatcggcag cacgtttcag gatctcgtca gaaacggcca
     9781 tttcaccggt gtagtcggtt gatgccaccc acagacgcag aatatccgcg cccagtttgt
     9841 tcatcacatc ctgcggcgaa acggtattgc cgatggattt agacatcttg cggccctgac
     9901 catccacggt aaagccgtgg gtcagtacct gacgatacgg cgctttgcct ttcatcgcgg
     9961 tggagatcat tagggaagac atgaaccaac cacggtgctg gtcagaacct tccagataca
    10021 tgtccgctgc gtgaccggca aattccggac gcacgtcaac aacagaaaag tgggtagatc
    10081 cggagtcaaa ccatacatcc aatgtgtccg gcactttcac gtactgatca gcttcatcgc
    10141 cgaggatctc tttcgcatca agatcccacc acgcctggat gccgtcaact tcaacgcgtt
    10201 ttgccacttc ttccattagt tcgagagtac gcggatgcag ctcttccgtg tctttgtgca
    10261 cgaacagtga catcggtacg ccccaggtgc gctgacggga gatacaccag tcaggacggt
    10321 tagcaaccat cgactcgata cgcgcctggc cccagtccgg gatccactgc acgcctttga
    10381 tctctttcag tgactgcgca cgcagacctt tctgatccat gctgacgaac cactgcggcg
    10441 tcgcgcggaa gatgatcggc gttttgtgac gccagcagca cggatagctg tgctgcattt
    10501 tctcaacgtg cagcagcgcg cctttttcct gcagcagcgc aacgacgatg tcgttcgctt
    10561 tgaagacgtt cacgccatcc agcgtcggat aagtgcccgg cagataagtg ccgtccgggc
    10621 caaccgggtt agcggtttcc aggccgtatt tctgaccgat cacatagtcg tccgggccgt
    10681 ggccaggcgc ggtgtgaacg gcaccggtac cggcatccag ggtaacgtga tcgccgagga
    10741 ttgccggaac gtcgaagccc ataaacggat gggtaaagcg cagcaactca agctccgcac
    10801 cttttaccgt gccgagaatg gtgtaatcgg tcacgccgat acgctgcatt acgctttcaa
    10861 ccagatcttt cgccagaatc acggcctgac cgtcgatctg caccagcgca tagtcgaaat
    10921 caggtgcaat agagattgcg cggttcgcag gcagagtcca cggcgtagtg gtccagatca
    10981 ccagcgagat tgggccgtta acgttgctta cggcaaattt tgctttcagt gcatcctgat
    11041 cgaccgcctg aaaagcaacg tcgatggacg gagaagtttt gtcgtaatac tcaacttccg
    11101 cttccgccag cgcagaacgg cagtcaacgc accagtgtac cggcttcgcg cctttgtgca
    11161 ggtgaccgtt gccgatgatt ttgcccagcg cgcggatgat gttggcttca gttttgaagt
    11221 ccatggtcag gtacgggtgc gaccagtcgc ccagcacgcc cagacggata aagtctttgc
    11281 gttgaccgtc aacctgggtc gccgcgtatt cgcggcactt ggcgcggaac tcggcggcgg
    11341 tgaatttctc acccggctta ccgtattctt gctctacttt cagctcgatc ggcagaccgt
    11401 ggcagtccca gccaggcaca tacggcgagt cataaccgga aagccctttg gacttcacga
    11461 taatgtcttt cagaatcttg ttaaccgagt gaccaatatg aatgctgcca ttcgcataag
    11521 gagggccatc atgcagaatg aaggtttttt tgcctttttt agccgcacgg atgatgccgt
    11581 acagatcatc atcagtccaa cgcgccagca ttcccggttc gcgcttggcg agatcgccac
    11641 gcatcgggaa ccctgtttcc ggcaaattca gggttgattt atagtcactc atcagattct
    11701 cggttccgtg tttcggtttg attacataac aggcttaagc cggttttgtt agcccaaaaa
    11761 attcgcgggc ggttaattca tcacgcgcaa tctgcgcttt cagttcgtcc agcgacgcaa
    11821 atcgctgctc attgcgtatt tttttacgca gcactacttg tatatggcga ccgtaaaggt
    11881 ccattgcaac atctaacaaa tgcacttcca gctgctggcg aataccggca accgttgggc
    11941 gtgttccgat gtttgccacg ccgggtaacg gcttttcacc gaggcccagc acttctacca
    12001 cataaacccc tttcaccggg gaaacctgac ggcgtagcgg tacattcgcc gtcgggaaac
    12061 ctatagtgcg ccctaattca tcaccgtgga ctacacgccc ggagatggca aacgggtgcc
    12121 ccagtaaact ttctgccaga gccagattgt catccgccag cgcctgacgc acagcggtgc
    12181 tgctaatacg cacgccacct tcgcaaaaag tttgcgtact agtgatatcg aagccgtatt
    12241 ccatgccagc tttctgtaat aacaagaaat cgccttcacg accagcgcca aagcggaaat
    12301 catcccctac ggcaagaaat tttacgcgca aatgcttcac cagaagatcg ctgatgaaat
    12361 tttgcgcggt taacgccgcg aaacgcctgt cgaaacgcac gcacagcacg taatcaacgc
    12421 cacactctgc aaggtaacgc agtttttccc gcagccgggt cagtcttgcc ggggctttat
    12481 cggtagcaaa cagttccagt ggttgaggtt caaaaagcat caccatcacc ggtaagttgc
    12541 gcttgcgccc ttcttcctgc aagccctgta acagcgcgcg atgaccgcga tgcacgccgt
    12601 cgaaattacc aatagtcagc acacaccctt cttgcggggc ctggctgaga ttatgtatgc
    12661 cgcgtatcag cttcatgtct ggctcaaaac agtgaaaatc gtccgagtat accttgtaca
    12721 gcggtcaagg ttaaccggcg attgagtacc gaaatcagaa agaagtggct tttcattgcc
    12781 atggcgcaaa tcacgggaag aaagtgaccg cctgctgcaa tttttatcgc ggaaaagctg
    12841 tattcacacc ccgcaagctg gtagaatcct gcgccatcac tacgtaacga gtgccggcac
    12901 attaacggcg cttatttgca caaatccatt gacaaaagaa ggctaaaagg gcatattcct
    12961 cggcctttga attgtccata tagaacacat ttgggagttg gaccttggct aatatcaaat
    13021 cagctaagaa gcgcgccatt cagtctgaaa aggctcgtaa gcacaacgca agccgtcgct
    13081 ctatgatgcg tactttcatc aagaaagtat acgcagctat cgaagctggc gacaaagctg
    13141 ctgcacagaa agcatttaac gaaatgcaac cgatcgtgga ccgtcaggct gctaaaggtc
    13201 tgatccacaa aaacaaagct gcacgtcata aggctaacct gactgcacag atcaacaaac
    13261 tggcttaatc gccaatttgc tgaagctttg tgaaaaagcc cgcgcaagcg ggttttttta
    13321 tgcctgctgc ttttatgatg cgtgagagaa cacactaccg gttgccagat agtcaaaaga
    13381 tgctggcttt tgcagacacg ccgttacact ttctgaactc ttcgtggctt aagaatcgta
    13441 gaaccatcgg ctgatacatt ctgaacaaga atatctact
//
LOCUS       DADLFA010000110        13367 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000110 DADLFA010000000
VERSION     DADLFA010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13367
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.110"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(171..1094)
                     /locus_tag="JQH65_002686"
     CDS             complement(171..1094)
                     /locus_tag="JQH65_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY6140057.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            1197..2282
                     /gene="dmlA"
                     /locus_tag="JQH65_002687"
     CDS             1197..2282
                     /gene="dmlA"
                     /locus_tag="JQH65_002687"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HAY6140058.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFDLSFEQMG
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YINLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTYATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            2532..4142
                     /gene="yeaV"
                     /locus_tag="JQH65_002688"
     CDS             2532..4142
                     /gene="yeaV"
                     /locus_tag="JQH65_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HAY6140059.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVIPAGTVFKGDN"
     gene            4174..5298
                     /gene="yeaW"
                     /locus_tag="JQH65_002689"
     CDS             4174..5298
                     /gene="yeaW"
                     /locus_tag="JQH65_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
                     /protein_id="HAY6140060.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTSANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            5354..6319
                     /gene="yeaX"
                     /locus_tag="JQH65_002690"
     CDS             5354..6319
                     /gene="yeaX"
                     /locus_tag="JQH65_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HAY6140061.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHVI
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGNRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(6373..7488)
                     /gene="rnd"
                     /locus_tag="JQH65_002691"
     CDS             complement(6373..7488)
                     /gene="rnd"
                     /locus_tag="JQH65_002691"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HAY6140062.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     SAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLTLVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(7569..9254)
                     /gene="fadD"
                     /locus_tag="JQH65_002692"
     CDS             complement(7569..9254)
                     /gene="fadD"
                     /locus_tag="JQH65_002692"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HAY6140063.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGVGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAVVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKAPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(9459..10040)
                     /gene="yeaY"
                     /locus_tag="JQH65_002693"
     CDS             complement(9459..10040)
                     /gene="yeaY"
                     /locus_tag="JQH65_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HAY6140064.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGRSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(10080..10775)
                     /gene="tsaB"
                     /locus_tag="JQH65_002694"
     CDS             complement(10080..10775)
                     /gene="tsaB"
                     /locus_tag="JQH65_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAY6140065.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPATEDMLPIACQIFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(10833..12743)
                     /locus_tag="JQH65_002695"
     CDS             complement(10833..12743)
                     /locus_tag="JQH65_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAY6140066.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            12875..13219
                     /locus_tag="JQH65_002696"
     CDS             12875..13219
                     /locus_tag="JQH65_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HAY6140067.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         3199 a         3554 c         3335 g         3279 t
ORIGIN      
        1 accagatagg tccagtaatt cagaaccccg tattcagcga acacattaac ctctttaatt
       61 atctttcgat catgcgcgat taaaggtgaa tatgctaacc aatctgtagc ggcttagaaa
      121 ggagaaaatc aggttttaac ccgatatcaa cccgataatt gaatcattaa tcaggcatgc
      181 tccagtgaaa aattcgggta gtgctcggca aaatactggc gtaaaaattc taccgttatc
      241 cgcactttcg ctgacgtcgc cagccttgaa acataaacgg cccagacgtt cgctggctgg
      301 taatattccg gtagcacttg cactaaatga ccactggcaa tgttttcgct aacatcccac
      361 caggaacgca gagcaatccc ctgcccatcg agacaccact gatggacaat ttctccatga
      421 ttagacgaca gtgggccggt gactttaatc gcatggtggc cttctttatt gcgtaattgc
      481 cagacaccaa aaggatgatc gcgctcttta atgaccaggc acggcaatgc agaaagatcg
      541 gttaaatgtt tcggcgcgcc atgttgcgca ataaattccg gcgacgcgca gagaatgcgg
      601 taattggtcg ccagtttgcg ggcgattaaa ttaggggcga tgtcatcgcc aatgcgaata
      661 tcgagatcga caccttcatt gaccaaatcg accagccggt cttccacatc aaaacgtaat
      721 tccagttgcg gatacgcctt cgccagcgct gataacgccg gagccaccac ctgtcgtcca
      781 aaaccaaagc tgctgataat acgcagcatc ccctgtggca cctgacgcac gtcagaaagt
      841 tcgtccatca tctgaccgac atcctgcaaa atccgctgcg cccattcata aatccgctct
      901 ccttcttcgg taatggtgac gcggcgggtg gtgcggtgta acaacaccac gtttagcgtt
      961 tgctccagca aggcgatgcg cttgctgacg aacgccggtg aaacgcccag ttcttccgcc
     1021 acggcggcaa aaccagcccg acgagccacc agcataaaga cgcgcaaatc attcagcagc
     1081 ggtaaattat tcatgattcg tgttttatgt ttcaccagtt acggggatta attccttttc
     1141 cgtcaattat aggatggtga tgttgtcaat tttgatggtc aggaagtgag aacccaatga
     1201 tgaaaacgat gcgtattgct gcgatcccgg gagacgggat tggtaaagaa gtccttcctg
     1261 aagggattcg cgtgttacag gctgccgctg agcgctgggg cttcgacttg agttttgagc
     1321 aaatggggtg ggcgagctgc gagtattaca gccatcacgg taaaatgatg ccggacgact
     1381 ggcatgagca acttagccgt ttcgacgcca tctattttgg tgccgtcggc tggccggata
     1441 ccgttccgga ccatatttcg ttgtggggtt cgctgctgaa atttcgtcgt gaattcgacc
     1501 agtacatcaa cctgcgcccg gttcgtctct ttcctggcgt tccctgcccg ctggcgggaa
     1561 aacagcctgg cgacatcgat ttttacgtgg tcagggaaaa caccgaaggc gaatattcct
     1621 cgctcggcgg tagagtgaat gaaggtacag agcatgaagt cgtcattcag gaatcggtat
     1681 ttacacgtcg tggtgtcgat cgcattttgc gttatgcctt cgaacttgcg caaagccgcc
     1741 cacgtaagac gctaacttat gccactaaat cgaacggttt agccatcagc atgccgtact
     1801 gggatgagcg agtggaagca atggccgaga attacccgga gatccgctgg gacaagcagc
     1861 atattgatat tctctgcgcg cgttttgtga tgcagccgga acgcttcgat gtggtggtgg
     1921 cgtccaattt gtttggcgat atcctttccg atcttggccc ggcctgcacc ggcaccattg
     1981 gcattgcccc atccgccaac ctgaatccgg aacgcacttt cccgtcgctc ttcgagcctg
     2041 tccacggttc cgcgccggat atctacggga aaaatattgc taaccctatc gccacgattt
     2101 gggccggggc aatgatgctc gattttctcg gcaatggcga tgagcgtttc cagcaagcgc
     2161 ataacggtat tctggcagca attgaagaag tgattgctca cgggccgaaa acacctgata
     2221 tgaaaggcaa tgccaccacg ccacaggttg ccgacgcgat ttgcaaaatt attttgcgtt
     2281 aaggtcaaac cagtttattt gaaccgcgtc actgacgcgg ttttttattc gttctttgca
     2341 gtaaataacc tgcgtcattt caccttttat tgtttccgtt tcgtgtttta tggctttccg
     2401 cattcttaat tgtttaattt atgtaatatt caaataatca atttgttaaa cacaacatac
     2461 aactcattac cctgcaaata attacaactg gcaaaagcca gagacgggct tttatgccta
     2521 ggagatgact catgatgagc aatgttaaga aaaaagatgt gccgctgata agcatcagcc
     2581 tggtggccat tcttttcatc gcagctgcat taagcctttt cccacaacaa tcggccgacg
     2641 cggccaacgc aatatacact tttgttacgc gtacgttagg ttccgccgta caggtattgg
     2701 ttttgctggc aatgggactg gtgatttatt tagccaccag taaatacggc aatattcgtc
     2761 ttggcgaagg aaaaccggaa tacagcacgc tctcctggct gtttatgttt atttgtgccg
     2821 gtttaggttc ttctacgctt tattgggggg ttgctgaatg ggcctattat tatcaaacac
     2881 ctggattaaa tatcgcaccg cgttcacaac aggcactcga atttagcgtt ccctactctt
     2941 tcttccactg gggcatcagc gcctgggcaa cttatacgct ggcctcatta atcatggctt
     3001 atcactttca tgtgcggaaa aacaaaggtc tgagcctttc cggcattatt gctgctatta
     3061 ccggcgttcg cccgcaaggc ccatggggaa aactggttga tttgatgttc ctgatcgcca
     3121 ctgtcggcgc actgactatt tcccttgttg tcaccgcagc aacctttact cgtggacttt
     3181 ccgcactgac aggtttaccc gacaacttca ccgtgcaggc atttgtgatc ctgctttctg
     3241 gcggcatttt ttgcctaagc tcgtggattg gtatcaacaa cggtttgcaa cgtctgagca
     3301 aaatggttgg ctggggcgcg ttcctgctgc cattactggt gctgattgtc ggcccaaccg
     3361 aatttattac caacagcatc atcaatgcca tcggcctgac cacgcaaaac ttcctgcaaa
     3421 tgagcttatt caccgatccg cttggcgatg gttcatttac ccgcaactgg accgttttct
     3481 actggctgtg gtggatctca tacacccctg gcgtagcaat gtttgtcacc cgcgtttccc
     3541 gcggtcgtaa gattaaagaa gttatctggg gactgatcct cggcagcacc gtcggttgct
     3601 ggttcttctt cggcgtaatg gaaagctatg ccattcatca gtttatcaat ggcgtaatca
     3661 acgtcccaca ggtgctggaa acactgggcg gcgaaacagc tgtacagcaa gttctgatgt
     3721 cgttgccagc cggtaaattg ttcctcgccg catacctggg cgtgatgatt attttccttg
     3781 cctcgcatat ggatgcagtg gcctacacca tggcggcgac cagtacgcgt aatctccagg
     3841 aaggtgacga tcctgaccgt gggctgcgtc ttttctggtg cgtggtgatc actctgatcc
     3901 cgctttccat cttgtttacc ggtgcttcgc tggaaacgat gaaaaccacc gtcgtgctca
     3961 cagcccttcc cttcctcgtc attttactgg tgaaagtcgg cggatttatt cgctggctga
     4021 aacaggatta cgccgacatt ccggctcatc aagttgaaca ttatctcccg cagacaccgg
     4081 ttgaagccct ggaaaaaaca ccagtgatcc ctgcgggaac cgtattcaaa ggcgacaact
     4141 gagcgccatc atcctaacga taaaggtatc cctatgagca atctgagccc tgactttgta
     4201 ctacccgaaa atttttgcgc taacccgcaa gaggcgtgga ccattcctgc ccgtttttat
     4261 accgatcaga acgcgtttga acacgaaaaa gagaacgtct tcgccaaaag ctggatttgc
     4321 gtcgctcaca gcagcgaact ggcgaatgcc aatgattatg tgacgcgtga gatcattggc
     4381 gaaagcatcg tgctggtacg cggtcgtgat aaggttttgc gcgcgttcta taacgtgtgt
     4441 ccgcaccgtg gtcatcagtt gttgagcggt gaaggaaaag caaaaaatgt gattacctgc
     4501 ccgtatcacg catgggcatt caaactcgat ggcaatctgg cccatgcacg taactgcgaa
     4561 aacgtcgcca atttcgatag cgacaaagcg caactggttc cggtgcgtct ggaagaatat
     4621 gccggattcg tcttcatcaa catggacccc aacgccacca gcgtagaaga tcagttaccc
     4681 ggtctgggcg cgaaagtgct ggaagcctgc ccggaagtcc acgatctgaa actggcggcc
     4741 cgctttacca cccgcacgtc tgccaactgg aagaacattg tcgataacta tctcgagtgc
     4801 tatcactgtg gtccggcgca tccaggtttc tccgactccg tacaggttga tcgttactgg
     4861 cacaccatgc acggtaactg gacgctgcaa tacggtttcg ccaaaccgtc cgaacagtcg
     4921 tttaaatttg aagagggtac ggatgcggca ttccacggtt tctggctgtg gccgtgcacg
     4981 atgctgaacg tcaccccgat caaagggatg atgacggtca tttatgaatt cccggtggat
     5041 tctgaaacta ccctgcaaaa ctacgatatt tacttcacca atgaagagtt aaccgacgag
     5101 caaaaatcgc tgattgagtg gtatcgcgat gtgttccgtc cggaagattt acgtctggtt
     5161 gaaagcgtac agaaagggct gaaatcgcgt ggctatcgtg gtcaggggcg catcatggcc
     5221 gacagtagcg gtagtggcat ttccgaacat ggtatcgccc atttccataa tctgctggcg
     5281 caggtgttta aggactaatg acatcggcgg cggtattttc cgctgctggg ctgatttttg
     5341 atggagtaca gcaatgtcag actatcaaat gtttgaagta caggtgagcc aggttgaacc
     5401 ccttaccgaa caggtgaaac gcttcacgct ggtggcaacc gatggcaaac cgctgcctgc
     5461 gttcaccgga ggaagtcacg tcattgtgca gatgagcgat ggtgataacc agtacagcaa
     5521 tgcgtattca ctactgagtt cgccgcatga cacctcttgt tatcagattg ccgttcggct
     5581 ggaggaaaac tcgcgcggcg gttcccgctt tttgcatcag caggtaaaag tgggcaatcg
     5641 gttaacgatt tcaacgccta ataacctgtt tgcgctaatt ccctcagcca gaaagcatct
     5701 gtttatcgcg ggcggtattg gtatcacccc tttcctgtcg cacatggcag agctgcaaca
     5761 cagcgacgtc gactggcagc tacattactg ctcgcgaaat ccagaaagtt gcgcatttcg
     5821 tgatgagcta gtccagcatc cgcaggctga gaaagttcac ttgcatcact catcaaccgg
     5881 aacacgactg gaattagcgc gattattggc ggatatcgaa cctggcacac acgtttatac
     5941 ctgtggtccc gaggcgctaa atgaagcggt aagaagtgaa gctgcgcgtc tggacatcgc
     6001 cgccgatacg ctgcactttg agcaatttgc tatcgaagac aaaaccggcg atgcctttac
     6061 cctggtgctt gcccgttccg gaaaagagtt tgtggtgccg gaagagatga ctattttgca
     6121 ggttattgaa aataataaag ccgcgaaagt ggaatgttta tgtcgtgaag gggtatgcgg
     6181 aacctgcgaa acagcaatac tggaaggtga agctgaccat cgggatcagt attttagcga
     6241 tgaagagcgt gccagccagc aaagtatgtt gatctgttgt tcgcgtgcga agggtaaacg
     6301 cctggtgttg gatttgtagt ttgccaaagc cggatgtggc gctgagcgcg cccagtccgg
     6361 cttcggaaga ttttactgcg gatattcctg caataaattg tgtaatgctt ccgccatcag
     6421 ctcaccacgc cagccggaaa tcagctccgg caaattgttc tgcggtttca gtttccagtg
     6481 ccagttcagc agttggttga tttgccgacg cgatgccagc aattcggcgc tgatcttatg
     6541 cgtttcgctc acgtcagtaa tcagcgactt aatcgcttta aacgctttac gataaccagg
     6601 catgtccatc aggttaagca tcggctgcgg taaggcctct tccggcaatg tctgcgcttt
     6661 ttccaccagc gtcagcagcg ttttaccgtg aaagcggatt tcgctaccgg ataaacccag
     6721 gctgtccagt tcgcctaaac ttcccggcat ataacgcgcg accgaccaca aatgctcttc
     6781 acgcacgaca aagttcaccg ccagatcacg ctctcgcgcc ttgcgcagtc gccagtcggc
     6841 taacagttgc agacaggcca gttggcgtgt gcgtaactgc caggcattgg tgatatcacg
     6901 ccaggcatct tccggcgcaa cgacttcctg acgacgcatt tgcatcaggc ggcattcatc
     6961 cagcgccgca ggtagccagc cggaggcctc cgtttctacc ataagcttgg cggtgatcgg
     7021 taacagatac cagacatccg ccgctgcgta ttcacactga cgttcggtca gcggtctggc
     7081 cagccagtcg gtgcgcgatt cactcttgtc cagcgtaacg ccggaatact cttccaccat
     7141 ggaagcgaaa ccccatgaca tcgggcgtcc gcagaaggca gaaaggattt gcgtgtcaat
     7201 caagggttgt ggtaattcgc caaagacatt gaggaacact tccagatctt cactgcctgc
     7261 atggagaaat tttgtgatgg acggatcgcg caggatcgct ttcagcggtg accagtcggt
     7321 gatcccgaga ggatcaatta gcgccagatg ctcgccatcg aaaagttgaa tcaaccccag
     7381 ctgcgggtaa taagtgcgcg tacgaacaaa ttcagtatcc agggctatcg ccggaaaggc
     7441 acggacggct tcacacaaag aagccagcgc atcgtccgtg gtaatcattt ggtaattcaa
     7501 attgttttct ctttagtggg cgtcaaaaaa acgccggatt aaccggcgtc tgacgactga
     7561 cttaacgctc aggctttatt gtccactttg ccgcgcgctt cgtcacgtaa ttctcgtcgc
     7621 aaaatttttc cgacgttaga tttcggtaac tcatcacgaa actccaccag cttcggtact
     7681 ttgtatcccg tgagctgacg gcggcaaaaa gtcaccagtg actcttcggt aagcgatgga
     7741 gcttttttca ctacgaagat tttcaccgct tcaccactgg agccggaagg tacgccaaca
     7801 gccgcgactt cctgtacgcc aggatgctgc atgacgacat cttcaatctc gttgggatag
     7861 acgttaaaac cggaaaccag aatcatgtct tttttacgat cgacaatgcg caggaatcct
     7921 tcttcatcca ttaccgcgat gtcgccggtg tgtaaccagc catttttgat gatttcatcg
     7981 gtagcatcgg gacgctgcca gtaacccagc atcacctgcg gtcctttgac acaaagctca
     8041 cccggttgac ctggtggtac ttcattatca tcatcatcca ccagtttggc ttccgtcgac
     8101 ggcaccggca aaccgatgct accactatga taatcaatat catatgggtt aacgctgacc
     8161 agcggcgcac actcggtaag gccatagcct tccagcagat actgtccggt cagtttcacc
     8221 caacgctctg ccaccacttg ctgcactggc atcccaccgc ctgcggaaag atgcagactg
     8281 gagaaatcca gctgctggaa ctctttattg ttcagcaacg cattgaacaa ggtgttaacg
     8341 ccagtgatag cggtaaacgg atatttcgct aactctttta ccaaccctgg aatatcacgc
     8401 gggttagtga taagcaggtt ctgcccaccc agttcgataa acagcaggca gttaatggtc
     8461 agggcaaaaa tgtgatacag cggcagtgcc gttactacca gttctttacc tggatgcagc
     8521 aacggcccat aggtggcgtt gacctgttcc aggttcgcca gcatattgcg gtgagtcagc
     8581 atcgcgcctt tcgccacacc tgtggtgccg ccggtgtatt gcaggaaggc taaatcttcc
     8641 ggcaccagtt cgggtttgac gtactgcatc cggtagccgt tatgcagtgc gctacgaaat
     8701 gaaatggcat ctggcagatg gtatttcggc accaaacgct tgatgtattt aacaacgaaa
     8761 ttgactaccg tgccttttgc cgtagatagc tgatcgccca tacgggtcag aattacgtgc
     8821 tgaacggcgg ttttatcaac cactttttcc agtgtgtgag caaagttaga cacgataaca
     8881 accgccgatg cgccgctatc gttaagctga tgctcaagct cacgcggggt atacaacggg
     8941 ttaacgttta cgacgatcat cccggcacgc aaaatgccaa acagcgccac cggatattgc
     9001 aataaattag gcatcatcaa cgcaacgcga tcgcctttct tcagccccac cccttgttgc
     9061 aaataagcgg caaacgcgcg actgcgttct tccagcttgc ggaaggtcat cacctccccc
     9121 atattcacaa acgcaggctg atcggcgtag cgcgcgaccg actgctcaaa catatctacc
     9181 agagattgat aacggtcagg gttgatctcc gtcggaacgt ccgcgggata acggttaagc
     9241 caaaccttct tcaattcttc acctctaaaa tgcgtgttcg tcgtcatcgc aaccccaaat
     9301 gatatacatg ccgttaacat aatattaact catcatacca gcttgataat tacccaacga
     9361 aaaggttgcg aagcgcgtca ctatttattt ttatctttac cgtaagaatg cagaaacagc
     9421 ggaccagccg ctgtttcttt ttctttaaaa acaagcaatt attcagttac aactgtttgt
     9481 actctcgcgg gaccgggatt ataccagccc catccgccgt agccataggg ccagccacga
     9541 ccgccataaa accatggatc aatcggctga ggcggcataa tcacctgctg ggttaaatgc
     9601 caacgtttgt aacccgttac ttgcatcacc ataaatttat agggcgtatt gccgattttg
     9661 ccgtcaaccg caccagtgat tggcccgact accgtaacca gttgtccacg gaaatccacc
     9721 gggtccagaa aaccgttcac atcggcataa atgcgaccgc gagaaggttc tcccagcgtc
     9781 ggtctggctc cgctgtccag cggtacggta gcaatttcca ggcgggtttt cccttgctgg
     9841 ttttgtaccg caaccacttt gccaccaaag cgtgcctcct gaccaacgta cagctgcggc
     9901 gcactcatca cccgaactaa atcttgttgc ggcgtgggac tgcggccttt aatggcgtcc
     9961 ggcacagtga cacaaccgct cagcattagc gcaaacgtac ctgccagtat gcctttgata
    10021 acattctttt gaaccgccat gatgcgactc ctttttttct cagagcatac tcttaagatt
    10081 cattctttgc ccggaagttt cttccatgcg acgttgttac gtaaataaac cggttcggca
    10141 tgttccaccg ccaccgtttt accctcggca aatatctgac acgcaatcgg cagcatatct
    10201 tccgtcgctg gcagtaaaac ttctccgtcg cgcaaaacca gcccgctctc tttaccgaga
    10261 tccggccagg cttgccagcc cgtccctacc gttacccatt cgccggaaag ctgttgcatt
    10321 cgttcatgaa cgagttcggg tttgagtacg gcttcggttt cttcaccgcg ccagataccg
    10381 ttttcatcac gctgatattc ggcccagtaa acttcgccca tccgcgcatc aatggcagcc
    10441 agcacgcggg ttgcgccgtt tttgcgccac gccccttgcg ccattgtcat tagcgtggag
    10501 acgccaatca tcggcagctc tgcgccaagt gccagccctt gcgcaatgcc aataccaatg
    10561 cgcacgccgg taaagctacc ggggccgcgc ccgtaagcca gagcgttaat atcagtcagg
    10621 gaagttccgc tggtggtcag gatatcctgc accatcggta agattcgttg agtatgttca
    10681 cgagggcaaa gctcaaaatg agcgttgaca gtaccgtcgt tccacagggc gacagagcag
    10741 gcctctgtcg cggtatcgat agccagaatt cgcatgggtc ttcgtgctta gatcaataaa
    10801 aaggcgcgca tcataccata ctccgtaaca aattacctgg aggatggtat cgcaaggaaa
    10861 cgaaccgcac gggcaatgtc acgggtgcgc ggcgcgggcg gcagactggc gagaaacgtc
    10921 gcgccgtaag ggcgcatcac cagccgattg tcacaaatca ccaaaacccc gcgatcgtcg
    10981 gcgtcgcgaa tcagtcgccc taccccctgc ttgagagtaa tcaccgcatc cggcagttgt
    11041 acttcatcga acgggtcgcc accgcgcaaa cggcaatctt ccatgcgcgc ttttaacagc
    11101 gggtcatccg gtgaggtaaa cggcaacttg tcgataatca ccaatgacaa tgtatcgcca
    11161 cgcacgtcca ccccttccca gaaactactg gttgccacaa gaagcgcgtt accggcgctg
    11221 acaaattgct gcaacagttg ccctttgctg gtttccccct gcaacaaaac gggaagcgtc
    11281 atggtagcgc ggaactgctc ggccagatca cgcatcatgg cgtgcgaggt acaaagcata
    11341 aaacaacgac cgttgttagc ttcgatgatc ggtcgcagca ttgccgctaa ctggcgagca
    11401 gaacctggct ggttggtttg cggcagattg cgcggcacac agagtaacgc ctggcggctg
    11461 taatcaaacg ggcttggcaa tagcaacgac tccgcctgtt ctatcccaag ccgcgaggtg
    11521 aaatgatgca gatcgtcgtt caccgacagc gttgctgagg tgaagatcca gctaccaggc
    11581 ttttgcgcca ttaactcttt gaatttatcc gccacgctga gcggcgtgag agccagagta
    11641 aaatggcgcg aagtgcattc gtaccagtag ctgtagcccg gctgattgat ctcttttagc
    11701 cgcttcagcc gtgtgcgata caacgtagcg cgctcaaatg ccgcatccag caaggcggaa
    11761 cgccccagtg acagtttcgc cacgtcataa caaagttcca gggtgtcatc gagcagtaaa
    11821 aatgcccgct gaatttgcgg attagctaac agctcacgca ggttaccgcg ataacctggc
    11881 tcaccgagtt gcagacgaaa atcctgcgca ctctgggcaa gacgatcagc gcacttttgt
    11941 aactgctggg tgtcttttaa ttcggtgcgg taggcgatgg tgatgtcttt tgccaggtcg
    12001 agcagttgtc gactggagag tgactgacca aaatactggc tggcaatgtc cggtagctgg
    12061 tgggcttcgt cgaagatcat gacgtccgct tccgggatca gttcgccaaa tccactctct
    12121 ttaaccacca tatccgccag aaagagatga tggtttacca ccaccacatc ggcgtccatc
    12181 gcttttttac gtgctttgac cacaaagcaa tctttataca tcgggcagtc gctgccaaga
    12241 cagttgtcgt tggtgctggt aaccagcggc cacgcctgtg aatcttccgc cacgctgacg
    12301 caggtgctga tatcaccatc gactgtttga ttagaccagg agcgcagcag gatcacatcg
    12361 cttaagattt gtaccggcag atcgcccccc gccagcgcct gctgttcgag acgttcgagg
    12421 cagaggtagt ttgagcgccc tttcagcagc gccacgttgc ccgtatattt caatgccttt
    12481 gagactgttg gcaaatcgcg gctgtagagc tgatcctgca acgcttttga gccggtcgag
    12541 ataatgactt tctttttcgc ccgcagcgca ggagccaggt aagcgtaggt tttgcccgta
    12601 ccggttcctg cttccaccac cagcggctgg cctttttcta tcgcctgggt gacggctacc
    12661 gccatctgtc gctgtggttc tcgcggctta aagcctggta tcgctttcgc cagctgaccg
    12721 tctggtgcaa aatcgtccgt cacactaccc cctgttgatt tgaacgggga ttatgtcagg
    12781 atgagggcgc tttcgccagt tgaagtggtg acggcaacct cacattgtgg cagtctttgc
    12841 agcacgaaaa tggaagtata atgaggacaa aatgatgact atcgttcgta tcgatgctga
    12901 agcccgctgg tctgatgtag taatccacaa caacacgctc tactacactg gtgtaccgga
    12961 aaacctcgac gccgatgctt ttgagcaaac cgccaacacg ctggcgcaga ttgacgccgt
    13021 gctggaaaaa cagggcagca ataaatcgag cattctggat gccaccattt tcctggctga
    13081 taaaaacgac ttcgcggcga tgaataaagc gtgggatgcc tgggttgtgg cgggtcatgc
    13141 gccggtgcgc tgcacggtgc aagcggggct gatgaacccg aagtataaag ttgaaattaa
    13201 gattgtggct gcggtgtaag ctttatcgaa gcaaaataag tcagacaatc atttatcgat
    13261 aatactggtc ggttttacat aaatcgacca gagattaaga tattactgtt gatgctcctg
    13321 ctaattcatt atttattttt ataatttcaa ttttagctat aaagcta
//
LOCUS       DADLFA010000111        13343 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000111 DADLFA010000000
VERSION     DADLFA010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13343
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.111"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            414..1616
                     /locus_tag="JQH65_002697"
     CDS             414..1616
                     /locus_tag="JQH65_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline/glycine betaine ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HAY6140068.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            1609..2673
                     /gene="proW"
                     /locus_tag="JQH65_002698"
     CDS             1609..2673
                     /gene="proW"
                     /locus_tag="JQH65_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HAY6140069.1"
                     /translation="MADQNNPWDTTPAADSAVQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPSIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            2731..3723
                     /gene="proX"
                     /locus_tag="JQH65_002699"
     CDS             2731..3723
                     /gene="proX"
                     /locus_tag="JQH65_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HAY6140070.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTLLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKEPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            4015..5199
                     /locus_tag="JQH65_002700"
     CDS             4015..5199
                     /locus_tag="JQH65_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY6140071.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            5323..6060
                     /locus_tag="JQH65_002701"
     CDS             5323..6060
                     /locus_tag="JQH65_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAY6140072.1"
                     /translation="MESPTPQPALGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            6050..6385
                     /gene="ygaH"
                     /locus_tag="JQH65_002702"
     CDS             6050..6385
                     /gene="ygaH"
                     /locus_tag="JQH65_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HAY6140073.1"
                     /translation="MSYEVLLLGLLVGVANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(6404..6505)
                     /locus_tag="JQH65_002703"
     CDS             complement(6404..6505)
                     /locus_tag="JQH65_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6140074.1"
                     /translation="MFNGRKRTIHLGMTSLISADIVTTVTNKVNVLF"
     gene            6476..7006
                     /gene="emrR"
                     /locus_tag="JQH65_002704"
     CDS             6476..7006
                     /gene="emrR"
                     /locus_tag="JQH65_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HAY6140075.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            7133..8305
                     /gene="emrA"
                     /locus_tag="JQH65_002705"
     CDS             7133..8305
                     /gene="emrA"
                     /locus_tag="JQH65_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HAY6140076.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQNHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPVQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            8322..9860
                     /gene="emrB"
                     /locus_tag="JQH65_002706"
     CDS             8322..9860
                     /gene="emrB"
                     /locus_tag="JQH65_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HAY6140077.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWECGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(9924..10439)
                     /gene="luxS"
                     /locus_tag="JQH65_002707"
     CDS             complement(9924..10439)
                     /gene="luxS"
                     /locus_tag="JQH65_002707"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAY6140078.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGSHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(10589..12145)
                     /gene="gshA"
                     /locus_tag="JQH65_002708"
     CDS             complement(10589..12145)
                     /gene="gshA"
                     /locus_tag="JQH65_002708"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HAY6140079.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTQATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCCDISGTDAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGKAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(12218..12646)
                     /locus_tag="JQH65_002709"
     CDS             complement(12218..12646)
                     /locus_tag="JQH65_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY6140080.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISYPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(12643..13198)
                     /gene="yqaB"
                     /locus_tag="JQH65_002710"
                     /pseudo
     CDS             complement(12643..13198)
                     /gene="yqaB"
                     /locus_tag="JQH65_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
BASE COUNT         3117 a         3483 c         3400 g         3343 t
ORIGIN      
        1 gctcctctta tgtgatgggg aaagcggttg aaacagaaga tgaagactgg aatttctgag
       61 ggtgttattt tcaaaaatat cactacccgc agcagggaaa taattcccgc caaatagctt
      121 tttatcacgc aaatattctg tagtgatcta cactgagact ctgttgcatt attcgcctga
      181 aactacaata ttcaggcgtt ttttcgctat ctatgacaaa aaatatcaac ttttctcgat
      241 ttgctctcag cccttatatc acgggaaatt ccggcgattt gctcgcatca atattcatgc
      301 cacatttgcc ctcaggggtt gcctcagatt ctcagtatgt tagggtagaa aaaagtgact
      361 atttccattg ggtaatatat cgacataaac aaataaagga atctttctat tgcatggcaa
      421 ttaaattaga aattaaaaat ctttataaaa tatttggcga gcatccacag cgagcgttca
      481 aatatatcga acaaggactt tcgaaagaac aaattctgga aaaaactggg ctatcgcttg
      541 gcgtaaaaga cgccagtctg gccattgaag aaggcgagat atttgtcatc atgggattat
      601 ccggctcggg taaatccaca atggtacgcc ttctcaatcg cctgattgaa cccacccgcg
      661 ggcaagtgct gattgatggt gtggatattg ccaaaatatc cgatgccgaa ctccgtgagg
      721 tgcgcagaaa aaagattgcg atggtcttcc agtcctttgc cttaatgccg catatgaccg
      781 tgctggacaa tactgcgttc ggtatggaat tggccggaat taatgccgaa gaacgccggg
      841 aaaaagccct tgatgcactg cgtcaggtcg ggctggaaaa ttatgcccac agctacccgg
      901 atgaactctc tggcgggatg cgtcaacgtg tgggattagc ccgcgcgtta gcgattaatc
      961 cggatatatt attaatggac gaagccttct cggcgctcga tccattaatt cgcaccgaga
     1021 tgcaggatga gctggtaaaa ttacaggcga aacatcagcg caccattgtc tttatttccc
     1081 acgatctcga tgaagctatg cgtattggcg accgaattgc cattatgcaa aatggtgaag
     1141 tggtacaggt cggcacaccg gatgaaattc tcaataatcc ggcgaatgat tatgtccgta
     1201 ccttcttccg tggcgttgat attagtcagg tattcagtgc gaaagatatt gcccgccgga
     1261 caccgaatgg cttaattcgt aaaacccctg gcttcggccc acgttcggca ctgaaattat
     1321 tgcaggatga agatcgcgaa tatggctacg ttatcgaacg cggtaataag tttgtcggcg
     1381 cagtttccat cgattcgctt aaaaccgcgt taacgcagca gcaaggtctt gatgcggcgc
     1441 tgattgatgc gccgttagca gtcgatgcac aaacgcctct tagcgagttg ctctctcatg
     1501 tcggacaggc accctgtgcg gtacccgtgg tcgacgagga ccaacagtat gtcggcatca
     1561 tttcgaaagg aatgctgctg cgcgctttag atcgtgaggg ggtaaataat ggctgatcaa
     1621 aataatccgt gggataccac gccagcggcg gacagtgccg tgcaatccgc agacgcctgg
     1681 ggtacaccgg cgactgcacc gactgacggc ggtggtgctg actggctgac cagtacgcct
     1741 gcgccaaacg tcgagcattt taatattctc gatccgttcc ataaaacgct gatcccgctc
     1801 gacagttggg tcactgaagg gatcgactgg gttgttaccc atttccgtcc cgtcttccag
     1861 ggcgtgcgcg ttccggttga ttatatcctc aacggtttcc agcaattgct gctgggtatg
     1921 cccgcaccgg tggcgattat cgttttcgct ctcatcgcct ggcagatttc cggggtcgga
     1981 atgggcgtgg cgacgctggt ttcgctgatt gccatcggcg caatcggtgc ctggtcgcag
     2041 gccatggtta ccctggcgct ggtgttaacc gccctgctgt tctgtatcgt cattggtttg
     2101 ccgctgggga tctggctggc gagaagtccg cgagcggcga aaattattcg tccactgctt
     2161 gatgccatgc agaccacgcc agcgtttgtt tatctggtgc caatcgtcat gctatttggt
     2221 atcggtaacg tgccgggcgt ggtggtgacg atcatctttg ctctgccgcc gattatccgt
     2281 ctgaccattc tggggattaa ccaggttccg gcggatctga ttgaagcctc gcgctcattc
     2341 ggtgccagcc cgcgccagat gctgttcaaa gttcagttac cgctggcgat gccgagcatt
     2401 atggcgggcg ttaaccagac gctgatgctg gccctttcta tggtggtcat tgcctcgatg
     2461 attgccgtcg gcgggctggg tcagatggta cttcgcggta tcggtcgtct ggatatgggg
     2521 cttgccaccg ttggcggcgt cgggattgtg atcctcgcca ttatcctcga tcgcctgacg
     2581 caggccgttg ggcgcgactc acgcagtcgc ggcaaccgtc gctggtacac cactggccct
     2641 gttggtctgc tgacccgccc attcattaag taactctgca cttgcccggt gacgccgggc
     2701 attatcaccc tgccaaaaaa aggaataaca atgcgacata gcgtactttt tgcgacagcg
     2761 tttgccacgc ttatctctac acaaactttt gctgccgatc tgccgggcaa aggtattact
     2821 gttaatccag ttcaaagcac catcaccgaa gaaaccttcc agacgctgct ggtcagccgt
     2881 gcgctggaga aattaggcta taccgtcaac aaacccagcg aagtagatta caacgttggc
     2941 tacaccttgc ttgcttccgg cgatgcaacc ttcaccgccg tgaactggac gccactgcat
     3001 gacaacatgt acgaagctgc cggtggcgat aagaaatttt atcgtgaagg ggtatttgtt
     3061 aacggcgcgg cacagggtta cctgatcgat aagaaaaccg ccgaccagta caaaatcacc
     3121 aacatcgcac aactgaaaga gccgaagatc gccaaactgt tcgataccaa cggcgacggt
     3181 aaagcggatt taaccggttg taatcctggc tggggctgcg aaggtgcgat caaccaccag
     3241 cttgccgcgt atgaactgac caacaccgtg acgcataatc aggggaacta cgcagcgatg
     3301 atggccgaca ccatcagtcg ctacaaagag ggcaaaccgg tgttttatta cacctggacg
     3361 ccgtactggg tgagtaatga gctgaagcca gggaaagatg tggtctggtt gcaggtgccg
     3421 ttctccgcac tgccgggcga taaaaatgcc gataccaaac tgccgaatgg cgcgaattac
     3481 ggcttcccgg tcagcaccat gcatatcgtt gccaacaaag cctggaccga gaaaaatccg
     3541 gcggcagcca aactgtttgc cattatgcag ttgccagtgg ccgatattaa cgcccagaac
     3601 gccattatgc atgacggcaa agcctcagaa ggcgatattc agggccacgt tgatggctgg
     3661 atcaaagccc accagcagca gttcgatggc tgggtgaatg aggcgctggc agcgcagaag
     3721 taatttttat tcaggcggat aaggcgtttt tgccttatcc gccgctctgt acacaatgcc
     3781 tgatgcgacg ctgacgcgtc ttatcatgcc tacaaaatac actcattccg tatggcggat
     3841 aaggcgtttt cgccgcatcc gccgttctgt gcacaatgcc tgatgcgacg ctggcgcgtc
     3901 ttatcatgcc tacaaaccac atcgcacatt tcaacaagct atctttcatc ccatattcat
     3961 caacatccgc tattattgat ttccagctta atcatcacct gatgaacaaa aataatgact
     4021 aaacctaatc atgagcttag cccggcgctg atcgtgctga tgtctatcgc caccggtctg
     4081 gcggtagcca gtaactatta cgcccagcca ttgctcgaca ccatcgcgcg taacttttcc
     4141 ctttccgcca gttcggcagg ctttattgtt accgccgcgc aattgggcta tgcagcaggt
     4201 ctgctgtttc ttgttcccct cggtgatatg tttgaacgcc gccgcctgat tgtctcgatg
     4261 accttactgg cggcaggcgg catgttaatc actgccagca gtcagtcgct ggcgatgatg
     4321 atcctcggta cggcattaac cggtttattc tctgtcgtgg cacaaattct ggttccgctg
     4381 gcagcgacgc tggcttcacc ggacaaacgc ggcaaagtgg ttggcactat tatgagcggt
     4441 ctgctgttgg ggatcttact cgcacggacc gttgccggat tgctggcgaa tctcggcggc
     4501 tggcgcaccg tcttttgggt tgcctcggtg ttaatggcac tgatggcgct ggcattatgg
     4561 cgtggtctgc cacaaatgaa atcagaaacc cacctcaact acccacagtt gttaggttcc
     4621 gttttcagca tgtttatcag cgataagatc ctgcgcaccc gcgcattgct gggctgcctg
     4681 acctttgcca acttcagcat tctctggacc tcaatggcct ttttgcttgc cgctccacct
     4741 tttaactaca gcgatggtgt cattggtctg tttggacttg cgggagctgc cggagcgttg
     4801 ggcgctcgtc cggcgggcgg ttttgccgat aagggtaaat cgcaccacac cacaactttc
     4861 ggtctgctgc tgctattact ttcatggctg gcgatctggt ttggtcacac ttccgtactg
     4921 gcgctgatta tcggcatcct ggtgctggat ctcaccgtac agggcgtgca tatcactaac
     4981 cagacggtaa tttatcgaat acaccctgat gcgcgtaatc gcctgaccgc aggttacatg
     5041 accagctact ttattggcgg tgccgccggt tcgctaattt cagcctcagc ctggcaacat
     5101 ggcggttggg ctggcgtttg tctggctggc gcgacgattg ccctggttaa cttactggtc
     5161 tggtggcgag gttttcatcg tcaggaagcc gcaaattaag caatcagcaa ccgttttcgt
     5221 ggctttacac ttataagggt gttaagaagc ccatcagtct gataaggtta agatattcat
     5281 tcagtctatt tataatatta acaatcgtta agcgtacact ctatggaaag ccctactcca
     5341 cagcctgctc ttggttcggc gaccttcatg gaaggatgca aagacagttt accgattgtt
     5401 attagttata ttccggtggc ctttgcgttc ggtctgaatg cgacccgtct gggattctct
     5461 cctctcgaaa gcgttttttt ctcctgcatc atttatgcag gcgcgagcca gttcgtcatt
     5521 accgcgatgc tggcagccgg gagtagtttg tgggttgctg cactgaccgt catggcaatg
     5581 gatgttcgcc atgtgttgta tggcccgtca ctgcgtagcc gtattattca gcgtctgcaa
     5641 aaatcgaaaa ccgccctgtg ggcatttggc ctgacggatg aggtttttgc cgccgcaacc
     5701 gcaaaactgg tacgcaataa tcgccgctgg agcgagaact ggatgatcgg cattgccttc
     5761 agttcatggt catcgtgggt atttggtacg gtaatagggg cattctccgg cagcggcttg
     5821 ctgcaaggtt atcccgccgt tgaagctgca ttaggtttta tgcttccggc actctttatg
     5881 agtttcctgc tcgcctcttt ccagcgcaaa caatctcttt gcgttaccgc agcgttagtt
     5941 ggtgcccttg caggcgtaac gctattttct attcccgtcg ccattctggc aggcattgtc
     6001 tgtggctgcc tcactgcgtt aatccaggca ttctggcaag gagcgcccga tgagctatga
     6061 ggttctgctg cttgggttac tggttggcgt ggcgaattat tgcttccgct atttgccgct
     6121 gcgcctgcgt gtgggtaatg cccgcccaac caaacgtggc gcggtaggta ttttgctcga
     6181 caccattggc atcgcctcga tatgcgctct gctggttgtc tctaccgcac cagaagtgat
     6241 gcacgataca cgccgtttcg tgcccacgct ggtcggcttc gcggtactgg gtgccagttt
     6301 ctataaaaca cgcagcatta tcatcccaac actgcttagt gcgctggcct atgggctcgc
     6361 ctggaaagtg atggcgatta tataacccac aagaatcatt tttctaaaac aatacattta
     6421 ctttatttgt tactgtcgtt actatatcgg ctgaaattaa tgaggtcata cccaaatgga
     6481 tagttcgttt acgcccattg aacaaatgct aaaatttcgc gccagccgcc acgaagattt
     6541 tccttatcag gagatccttc tgactcgtct ttgcatgcac atgcaaagca agctgctgga
     6601 gaaccgcaat aaaatgctga aggctcaggg gattaacgag acgttgttta tggcgttgat
     6661 tacgctggag tctcaggaaa accacagtat tcagccttct gaattaagtt gtgctcttgg
     6721 atcatcccgt accaacgcga cgcgtattgc cgatgaactg gaaaaacgcg gttggatcga
     6781 acgtcgtgaa agcgataacg atcgccgctg cctgcatctg caattaacgg aaaaaggtca
     6841 cgagtttttg cgcgaggttt taccaccgca gcataactgc ctgcatcaac tctggtccgc
     6901 gctcagcaca acagaaaaag atcagctcga gcaaatcacc cgcaaattgc tctcccgtct
     6961 cgaccagatg gaacaagacg gtgtggttct cgaagcgatg agctaacgcg tcatctcgct
     7021 caaaaatcca gatttataaa agaaaaaatg accggccagc atcgcaacat gctggccttt
     7081 ttggcaagca ggtcggctca gccgatgagt taagaagatc gtggagaaca atatgagcgc
     7141 aaatgcggag actcaaaccc cgcagcaacc ggtaaagaag agcggcaaac gtaagcgtct
     7201 gctcctcctt ctcaccttgc tctttataat tattgccgta gcgataggga tttattggtt
     7261 tttggtactg cgtcatttcg aagaaaccga tgacgcatac gtggcaggga atcagattca
     7321 aattatgtct caggtgtctg gcagcgtgac gaaagtctgg gccgataaca ccgattttgt
     7381 aaaagaaggc gatgttctgg tcactctcga cccgacagat gctcgccagg cgtttgaaaa
     7441 agccaaaact gcactggctt ccagcgttcg ccaaaaccac cagctgatga ttaacagcaa
     7501 acagttacag gcgaatattg aggtgcaaaa aatcgccctc gcgaaagcac aaagcgacta
     7561 caaccgccgt gtgccgctgg gcaatgccaa cctgattggt cgcgaagagc tgcaacacgc
     7621 ccgcgacgcc gtcaccagtg cccaggcgca actggacgtc gctattcaac aatacaatgc
     7681 caatcaggcg atgattctgg ggactaaact ggaagatcag cctgccgtgc aacaggctgc
     7741 caccgaagta cgtaacgcct ggctggcgct ggagcgtacc cgtattgtca gtccgatgac
     7801 cggttatgtc tcccgccgcg cggtacagcc tggggcgcaa attagcccaa cgacgccgct
     7861 gatggcggtc gttccagcca ccaatatgtg ggtggatgcc aactttaaag agacgcagat
     7921 tgccaatatg cgtatcggtc agccggtcac tatcaccacg gatatttacg gcgatgatgt
     7981 gaaatacacc ggtaaagtgg ttggtctgga tatgggcaca ggtagcgcat tctcactgct
     8041 tccggtgcaa aatgcgaccg gtaactggat caaagtcgtt cagcgtctac ctgtgcgtat
     8101 cgaactggac cagaaacagc tggagcaata cccgctgcgc attggtttgt ccacgctggt
     8161 gagcgtcaat accactaacc gtgacggtca ggtactggca aataaagtac gttcaacacc
     8221 ggtcgcggta agtaccgcgc gtgaaatcag cctggcacct gtcaataaac tgatcgacga
     8281 tatcgtaaaa gctaacgctg gctaatccag aggtgcgtgt gatgcaacag caaaaaccgc
     8341 tggaaggcgc gcaactggtc attatgacga ttgcgctgtc actggcgaca ttcatgcagg
     8401 tgctggactc caccattgct aacgtggcga tccccactat cgccgggaat ctgggctcat
     8461 cgctcagcca gggaacgtgg gtaatcactt ctttcggggt ggcgaatgcc atctcgatcc
     8521 cgcttaccgg ctggctggca aagcgcgtcg gggaagtgaa actgttcctt tggtccacca
     8581 tcgcctttgc tattgcgtcg tgggagtgtg gtgtctccag cagcctgaat atgctgatct
     8641 tcttccgtgt gattcagggg attgtcgctg ggccgttgat cccactttcg caaagtcttt
     8701 tgctgaataa ttacccgcca gccaaacgct caatcgcgct ggcgttgtgg tcgatgacgg
     8761 tgattgtcgc gccaatttgc ggcccaatcc tcggcggtta tatcagcgat aattaccact
     8821 ggggctggat attcttcatc aacgtgccaa ttggcgtggc ggtggtgttg atgacactgc
     8881 aaaccctgcg cggacgcgaa acccgcaccg aacggcggcg gattgatgcc gtggggctgg
     8941 cactgctggt tattggtatc ggtagcctgc agattatgct cgaccgcggt aaagagctgg
     9001 actggttttc atcacaggaa attatcattc ttaccgtggt ggcggtggtg gctatctgct
     9061 tcctgattgt ctgggagctg accgacgata acccgatagt cgatctgtcg ttgtttaagt
     9121 cgcgcaactt caccatcggc tgcttgtgta tcagcctcgc gtatatgctc tacttcggcg
     9181 cgattgttct gctgccgcag ttgttgcagg aggtctacgg ttacacggcg acctgggcag
     9241 gtttggcctc tgcgccggta gggattattc cggtgatcct gtcgccgatt atcggccgct
     9301 tcgcgcataa actggatatg cggcggctgg taaccttcag ctttattatg tatgccgtct
     9361 gcttctactg gcgtgcctat acctttgaac caggtatgga ttttggcgcg tcggcctggc
     9421 cgcagtttat ccaggggttt gcggtggcct gcttctttat gccgctgacc accattacgc
     9481 tgtctggttt gccaccggaa cgactggcgg cggcatcgag cctctctaac tttacgcgaa
     9541 cgctggcggg gtctatcggc acgtcgataa ccacgaccat gtggaccaac cgcgagtcga
     9601 tgcaccatgc gcagttgact gagtcggtaa acccgttcaa cccgaatgcc caggcgatgt
     9661 acagtcaact ggaagggctt gggatgacgc aacagcaggc atcaggctgg attgcccagc
     9721 agatcactaa tcaggggctg attatttccg ccaatgagat cttctggatg tcagccggga
     9781 tattcctcgt cctgctgggg ctggtgtggt ttgctaaacc gccctttggc gcaggtggcg
     9841 gcggaggtgg tgcgcactaa gtacaactaa gccagttcat ttgaactggc ttttttcaat
     9901 taattgtgaa gatagtttac tgactaaatg tgcagttcct gcaacttctc tttcggcagt
     9961 gccagttctt cgttgctgtt gatgcgtacg tcacgttcca gaatgctacg cgcaatatcc
    10021 tgcgcttcct gcaacgagtg catctggtaa gtgccacact ggtagacgtt cagctccggg
    10081 atctgattct gatcctgcac tttcagcacg tcttccattg ccgctttcca ggcatcagca
    10141 acacgctgct catctggcgt accaatcaga ctcatataaa aaccggtgcg gcagcccatt
    10201 ggcgagatat cgataatctc tacaccatta ccgttaagat ggttacgcat aaaaccagca
    10261 aacaggtgct ccagagtatg gctccctctt tctggcatca cttctttgtt cggcacgcag
    10321 aagcgcagat cgaacacggt gattgcgtcg ccatgcgggg tgttcattgt tttcgccacc
    10381 cgaactgcag gcgcttccat ccgggtatga tcgactgtga agctatctaa caacggcatt
    10441 tagccacctc cggtaatttt tttaaaaatt ttctgaactc tttcttccca ggcgagtctg
    10501 agtatatgaa agacgcgcat ttgttatcat catccctgaa ttcagagatg aaattttggc
    10561 cactcacgag tggccttttt cttttctgtc aggcgtgttt ttccagccac accgcaaacg
    10621 gttcggtatc agcggtttcc atttcctgct gacggcgttc agatgcctcg cgctcggcta
    10681 caaaatcctc ttcgcgcaga atttccaacg gctcttcacg cagcagatta cggtaggctt
    10741 ctgcaaacgc tttgcctgtt ccgccaatac cagtatcaat catagacctt aagatacggg
    10801 cagagaaagt cagatcggga ttatcgaagc aggcgaccag ttcatcacac actttctgat
    10861 acgctttgcc gccgttaatg ctatccaacg tttgcgcgac gcgtttcaga tcgcggaaca
    10921 gatctttacc cacctgcggt aacgggaact gcgcggtttc gcagccgata cccagcgtca
    10981 ggcccggttt gcgaccttcg aggatcaccc ggttccagtt aacgcgtgta caggcaagtt
    11041 cgctactgct catttccggt gcatcagcca gcgcacacca gaccataaac aggtcgagga
    11101 atcgcacctg ctgttcatct acaccaatcg gcgagaacgg gttgatgtcc agcgaacgca
    11161 cttcaatata ttcaatgccg ccacgtaaca gcgcatcaga aggtgactcg ccgctgcggg
    11221 taacgcgttt tggacgaatc ggcgcataca gttcgttttc aatctgcagc acgttgctat
    11281 tgatttgcag cctcttaccg tctttctcaa taccaatctt cgcgtactct tccgatggcg
    11341 ttttgattgc ctgtttaagg cccgctacgt actcgtaaag atcgttgaag gtaataccaa
    11401 gattgctttg cgatttattg gtatagccga gatcgctcaa acgaagagag gtcgcatacg
    11461 gcaggtaata cataccgcac tcggttttct caaacggcag cgacgttggt tttccttgca
    11521 ggaaagaaga acaaatcgcc ggagatgcac caaacagata aggaatgacc caaccgaaac
    11581 gatagtaatt gcggataacg cggaaatagc ccgcagaaat tttctctttg gcatcagtgc
    11641 ccgagatatc acagcacttc gcttgccaga atgccattgg caaagagaaa ttgtagtgca
    11701 cgccggaaat ggtttgcatc agcgcgccgt agcgattttt cagcccttca cgatacagcg
    11761 ttttaaagcg tccggtgtta gaagtgccgt actgtgccag ttcgatgtcc tgaccttctg
    11821 cgatgtagca tggcatactt aacggccaca tccgctcatc gcccatattg cgcgccgtat
    11881 aacgatgcag atcgcgcata aaggtcaaca tatgttcaat atcaccatcc actggtgtaa
    11941 tgaattccag caatgcttcc gcaaaatcgg tagtaatcca tttgtgcgtc agtgcggaac
    12001 ctaatgcttc aggatgacct gttgttgcct gtgtgccatc agcattaaca cgcaaagttt
    12061 cgcgctccag cccacgctgt atccccttta acgcctgagg atgtttttcc agccaggcca
    12121 gcgcctgtga tacgtccggg atcaaattga cctcccgcct gtcaaaatcg ttttaattag
    12181 cataactgta atggtgacca tatgtgcagg cctacaatta gtgccaccac atcatgccct
    12241 gaacggtagc tgctgcaact gcaacatagc gtaaagcttt accaaggcac aaaaaaaaga
    12301 ttaccggtcc ccacgagatg cgcatccatc ccgctaacag acacagtaaa tcgccaacca
    12361 cgggcatcca gcttaataat agcgtgactg caccatagcg tttcagccag ccagtagctt
    12421 tctcttgcca gcgcgatgtt ttacgcaatg gaaagaaacg cccaaggata acgttagtta
    12481 accctccaag gctattaccc attgttgctg ttaaaactaa aacccaggga taactgatcc
    12541 cggaaagcaa cattgccacc agcacgactt cggagttgcc gggtaatagt gtagcgctga
    12601 gaaaactact ggcaaacaac gagaaaagcg ataacgcttc actcacagca agcgaacatc
    12661 cacggcgtcc atgcctgctg cacgggccgc ctgaataccg aaatcggcat cttcaaagac
    12721 cacacactgc gtcggttgca cgcccatacg ctgcgcgcac aacaaaaatg tgtctggcgc
    12781 gggtttatgg tgtttgacgt gatcagcagc gacgacggcg tcaaaataac ggcgtaatcc
    12841 caggtgcgcc agcaatgcct cagcgatggc gctttcactc cccgttccta cagccattgg
    12901 gcgacgacca tgccaacttt tcaccacttc aacaagagga agcggctcga cactatccag
    12961 cagcatactt cttactgctt ctgttttttc acgcgctaac gcatgcgggg cgagatcggc
    13021 ctgattcagc tcaataattg cctgagcaat acgccaggtg ggcgatccat taagcgcaat
    13081 cattgcctga acatcgtact gaagaccgta gtgccctaat acttcgcgcc acgctttacg
    13141 gtgcgtaggc tccgtatcca ggaatatcaa aaattaaacc tgcataacgc tcgtacatgg
    13201 tcttctcgca agtcgaaaaa tatgacgcta ctttagcgta attgcttgat tttgtcgctg
    13261 atggcgaagg agaagaaaat tcaggaaatg gataaagtag ttatttagaa atgagatatt
    13321 tttgaaggaa atttttggga gaa
//
LOCUS       DADLFA010000112        13313 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000112 DADLFA010000000
VERSION     DADLFA010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13313
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.112"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..309
                     /locus_tag="JQH65_002711"
     CDS             <1..309
                     /locus_tag="JQH65_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6140081.1"
                     /translation="VVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINGLQF
                     FDQQIRWAKADLVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAILLIN
                     "
     gene            508..789
                     /gene="ppiC"
                     /locus_tag="JQH65_002712"
     CDS             508..789
                     /gene="ppiC"
                     /locus_tag="JQH65_002712"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HAY6140082.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(876..2351)
                     /gene="ilvC"
                     /locus_tag="JQH65_002713"
     CDS             complement(876..2351)
                     /gene="ilvC"
                     /locus_tag="JQH65_002713"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HAY6140083.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRCHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            2501..3391
                     /gene="ilvY"
                     /locus_tag="JQH65_002714"
     CDS             2501..3391
                     /gene="ilvY"
                     /locus_tag="JQH65_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HAY6140084.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSSEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(3388..4932)
                     /gene="ilvA"
                     /locus_tag="JQH65_002715"
     CDS             complement(3388..4932)
                     /gene="ilvA"
                     /locus_tag="JQH65_002715"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HAY6140085.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRAVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(4935..6785)
                     /gene="ilvD"
                     /locus_tag="JQH65_002716"
     CDS             complement(4935..6785)
                     /gene="ilvD"
                     /locus_tag="JQH65_002716"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HAY6140086.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(6850..7779)
                     /gene="ilvE"
                     /locus_tag="JQH65_002717"
     CDS             complement(6850..7779)
                     /gene="ilvE"
                     /locus_tag="JQH65_002717"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HAY6140087.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(7799..8062)
                     /gene="ilvM"
                     /locus_tag="JQH65_002718"
     CDS             complement(7799..8062)
                     /gene="ilvM"
                     /locus_tag="JQH65_002718"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HAY6140088.1"
                     /translation="MMQYQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(8059..9705)
                     /locus_tag="JQH65_002719"
     CDS             complement(8059..9705)
                     /locus_tag="JQH65_002719"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HAY6140089.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAATGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPELQQPLNINDWQQYC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(9708..9758)
                     /locus_tag="JQH65_002720"
     CDS             complement(9708..9758)
                     /locus_tag="JQH65_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6140090.1"
                     /translation="MNNSTKFCFSTFSTGN"
     gene            complement(9845..9943)
                     /gene="ilvL"
                     /locus_tag="JQH65_002721"
     CDS             complement(9845..9943)
                     /gene="ilvL"
                     /locus_tag="JQH65_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HAY6140091.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            10295..11815
                     /locus_tag="JQH65_002722"
                     /pseudo
     CDS             10295..11815
                     /locus_tag="JQH65_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(11840..12178)
                     /gene="maoP"
                     /locus_tag="JQH65_002723"
     CDS             complement(11840..12178)
                     /gene="maoP"
                     /locus_tag="JQH65_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HAY6140092.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            12297..13136
                     /gene="hdfR"
                     /locus_tag="JQH65_002724"
     CDS             12297..13136
                     /gene="hdfR"
                     /locus_tag="JQH65_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HAY6140093.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(13232..13307)
                     /locus_tag="JQH65_002725"
     tRNA            complement(13232..13307)
                     /locus_tag="JQH65_002725"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13272..13274),aa:Trp,seq:cca)
BASE COUNT         3089 a         3601 c         3451 g         3172 t
ORIGIN      
        1 gtcgtgctca acggaaaaat taatggcaac gcatgtatcg tcgttccatt atcaaccact
       61 cgcgatcatg acaagttaaa tcgtggaatg catgttgaaa tagccagtaa tgttatcaat
      121 ggcctgcaat tctttgatca gcagattcgt tgggccaaag ccgacctggt acaacaagtc
      181 agtcgaaacc gattaaatag agcaaggact tacagagggt atcttaatca atgtttgcca
      241 catgagttgg ttgcagacat acagagggcg gtcatcaagt caatcaatgc catattgctc
      301 ataaattgac aataaaataa ccatccccga taatgacttt gtagcccata gaggccctaa
      361 gttttctccc cttgagggac ctgcgaagcc cggattttcc gggcttttgc catatatgta
      421 ccagcccctc cctgactgac aattacgtta gcatctgcgg atcatgctaa aatcgccgcc
      481 tgacaattat tcactcaagg cacgatcatg gcaaaaacag cagcagcact gcatatcctt
      541 gtaaaagaag agaaactggc tctggatctt ctcgagcaga ttaagaacgg tgccgatttc
      601 ggcaagctgg cgaagaaaca ctccatttgc ccatcaggca aacgcggcgg tgatttaggt
      661 gaattccgcc agggtcagat ggttccggcg ttcgataaag ttgtgttctc ttgtccggta
      721 ctggagccga ccggcccgct gcacacccag ttcggttacc acatcattaa ggtgctgtac
      781 cgtaactaat agcaaggcct tctccaggag aaggccttga gtgttttctc cctctccctg
      841 tgggagaggg tcggggtgag ggcatcagcg cgcacttaac ccgcaactgc aatacgtttc
      901 atatccgtca tatagccgcg cagtttctta cctacctgct caatcgcatg gcagcgaatc
      961 gcttcgttca catcacgcag ttgcgcgtta tctaccgcac cttccggaat agctttaccc
     1021 agatcgcccg gttgcagctc tgccataaac ggtttcagca acggcacaca agcgtaagag
     1081 aacagatagt taccgtactc agcggtatca gagataacca cgttcatttc gtacagacgc
     1141 ttacgggcga tggtgttggc aatcagcggc agctcgtgca gtgattcata atatgcagac
     1201 tcttcaatga tgccggaatc gaccatggtt tcaaacgcca gctcaacgcc cgctttcacc
     1261 atcgcgatca tcagtacgcc tttatcgaag tactcctgct cgccgatttt gccttcatac
     1321 tgcggcgcgg tttcgaaggc ggttttgccg gtctcttcac gccaggtcag cagtttctta
     1381 tcatcgttgg cccagtccgc catcataccg gaggagaatt cgccggagat gatgtcgtcc
     1441 atatgtttct ggaacagggg tgccatgatc tctttcagct gttcagacag cgcgtaagca
     1501 cgcagtttcg ccgggttaga gagacggtcc atcatcaggg tgatgccgcc ctgcttcagc
     1561 gcttcggtga tggtttccca accgaactga attagttttt ctgcgtatgc cggatcggtg
     1621 ccctcttcca ccagcttgtc gaagcacagc agcgagccag cctgcaacat accgcacagg
     1681 atggtttgct cacccatcag gtcagatttc acttccgcaa cgaaggacga ttccagcacg
     1741 cccgcacggt gaccaccggt tgctgccgcc catgctttag cgatcgccat gccttcgcct
     1801 ttcggatcgt tttccgggtg aacggcaatc agcgtcggta cgccgaatcc acgtttgtac
     1861 tcttcacgca cttcggtgcc agggcatttc ggcgcaacca tgactacggt gatgtcttta
     1921 cggatctgct cgcccacttc gacgatgttg aaaccgtgcg agtaacccag cgccgcgccg
     1981 tctttcatca gtggctgtac ggtgcgcact acgtcggagt gctgcttgtc tggcgtcagg
     2041 ttaaccacca aatccgcctg cgggatcagt tcttcgtaag tgcccacttt aaaaccgttt
     2101 tcggtcgctt tacgccagga tgcgcgcttc tcggcaatcg cttctttacg cagagcgtag
     2161 gagatatcga gaccagaatc acgcatgttc aggccctggt tcagcccctg tgcgccacag
     2221 ccgacgataa ccactttttt accctgaagg tagctcgcgc cgtcggcgaa ttcgtcgcgc
     2281 cccataaagc gacatttgcc cagctgtgcc agctgctggc gcaggttcag tgtattgaag
     2341 tagttagcca tggtgattcc tcgtgatgtt gtgcttctta ttgttcggtt cgcgtttgcg
     2401 agattgaatt cactatatga caggaaattt attgcggaaa ttgatatatt cacaacgtca
     2461 cattgcaatt tctgcaacgt caacattgag ggctgctcca ttggatttac gcgatctgaa
     2521 aaccttcctg catctggcgg aaagccgcca ttttggccgc agcgcgcggg cgatgcacgt
     2581 tagcccatcc acgctctcac ggcagattca gcgcctggaa gaagatcttg gtcagccgct
     2641 atttgtgcgc gataaccgca cggtgacgct gactgaagcg ggcgaagagc tgcgcgtttt
     2701 cgcccagcaa acgctgttgc agtatcagca gttgcgccac accatcgatc agcaagggcc
     2761 gtcgctctct ggcgaattac atatcttctg ctcggtgacc gctgcctaca gccatctgcc
     2821 accgattctc gaccgcttcc gcgcggaaca cccgtcggtg gagatcaaac taaccactgg
     2881 tgatgcggca gatgcgatgg aaaaggtagt cactggcgaa gcggatctgg cgattgccgg
     2941 gaaaccggag actctgcccg gcgcagtggc gttttcgatg ctggagaatc tggcagtagt
     3001 gctgattgcg cccgcgctgc cctgcccggt gcgtaaccag gtttcgtcag agaagccgga
     3061 ctggtctaca gtgccgttta tcatggccga tcagggaccg gtacgccgcc gcattgaact
     3121 gtggtttcga cgcaataaaa tcagcaaccc gatgatttac gccacggttg gcgggcatga
     3181 agcgatggta tcgatggtgg cgctcggctg tggcgtggcg ctgttgccgg aagtagtgct
     3241 ggaaaacagc cccgaaccgg tgcgtaaccg cgtgatgatt ttagagcgca gcgatgaaaa
     3301 aacgccgttt gaactgggcg tttgtgcgca gaaaaagcgg ctgcatgagc cgctgattga
     3361 ggcgttctgg aagattttgc cgaaccatta acccgccaaa aagaacctga acgccggatt
     3421 attggtttcg tcgtggcaat cgtagcccag ctcatttagc cgggtttcga aatccggttc
     3481 atggtcgccg agttcgaacg ccgccagtac gcgcccgtag tcggtgccat ggctgcgata
     3541 gtggaacaaa gaaatgttcc agtgcgtacc cagcgtgttg aggaagcgca gcagcgcgcc
     3601 cggtgattcc gggaattcga agctgtagag gcgttcctgc aacggatgtg acggacgccc
     3661 gccgaccata tagcgcacat gcaacttcgc catttcgtcg tcggagagat ccaccacgct
     3721 gtagccgccg tcgttgagca tctgcaaaat ttctttgcgc tcttcgaggc cgcggctcag
     3781 gcgcacgccg acaaagatgc aggcgttttt ggcatcggca aaacggtagt tgaactcggt
     3841 gaccgctcgc ccgccaagca gttggcagaa tttgaggaag ctgccttttt cttccggaat
     3901 ggtcaccgcc aacaacgctt cacgctgttc gcccagttcg cagcgttccg agacgtagcg
     3961 caggccgtgg aagttcacgt tggcaccgga aagaatatgc gccagccgtt cgccgcgaat
     4021 gttgtgctgg gcgatatatt ttttcattcc cgccagcgcc agcgcgccag agggttccgc
     4081 caccgcgcgc acatcttcga ataaatcctt catcgccgca cagatcgcat cactatcgac
     4141 ggtgatgatg tcgtcgagat actcctggca taaacggaag gtttcgtcac cgatgcgttt
     4201 taccgctacg ccttcagcaa atagccctac gcgcggcaga tcaaccggat gacccgcatc
     4261 cagcgctgct ttcaggcagg cggagtcttc cgcttctacg gcgatcactt tgatttgtgg
     4321 catcagttgt ttgatcagca ccgcaacgcc cgccgccaga ccgccgccgc cgactggcac
     4381 aaatacgcgg tcgagatggg cgtcctgctg gagcagttcc agcgccagcg tgccttgccc
     4441 ggcaatcacc atcggatggt cgaacggcgg cacccaggtg aacccctgct gctgtgacag
     4501 ttcgatcgct ttagctttcg cttcgtcaaa gttcgcgcca tgaagcagca cttcgccgcc
     4561 gaatccacgt accgcatcga ctttgatgtc ggcggtggcg gttggcataa cgatcagggc
     4621 cttcacgcct aaccgcgcag aagaaaacgc gacgccctgc gcgtggttac ccgcagaagc
     4681 agtgatcacg ccgtgcgctt tttgttcttc cgtcaggccc gccatcatgg cgtatgcgcc
     4741 gcgcagctta aagctgtgca ctggctggcg atcttcgcgc ttcaccagaa tgacgttatc
     4801 aagacgcgac gacagttttt ccattttttg tagcggcgta acctgcgctg cctcgtaaac
     4861 cggcgcgcgc agcactgctc ttaaatattc ggcaccttcc ggagcaccag acaggggttg
     4921 cgagtcagcc attattaacc ccccagtttc gatttatcgc gcaccgcacc tttgtcggcg
     4981 ctggttgcca ggctggcgta ggcgcgcagg gcaaaggaaa cctgacgttc acggtttttc
     5041 ggcgtccagg ctttgtcacc ccgagcttcc tgcgcttcac gacgcgccgc cagttcggca
     5101 tcgcttacct gtaactgaat gccacggttc gggatgtcga tagcgatcag gtcaccatct
     5161 tcaatcagac caatgctgcc gccgcttgcc gcttccggtg agacgtggcc gatggaaaga
     5221 ccagaggtgc cgccagagaa acgaccgtcg gtgatcagcg cacaggcttt accgagaccc
     5281 attgatttca ggaagctggt tgggtagagc atttcctgca tgccagggcc gcctttcggg
     5341 ccttcatagc ggatcaccac cacatcgccc gcgacaactt tgccaccgag gatcgcttct
     5401 accgcgtcgt cctggctttc gtacactttt gccgggccgg tgaatttgag gatgctgtca
     5461 tcgacgcccg cggttttcac gatgcagccg ttttcagcaa aattaccgta gagcaccgcc
     5521 aggccgccgt ctttgctgta ggcgtgttcc agcgagcgga tacagccgtt tgcgcgatcg
     5581 tcgtccagcg tatcccaacg gcaatcttgc gagaatgcct gggtggtacg gatacccgca
     5641 gggccggcgc ggaacatatt ttttaccgcg tcatcctggg tcagcataac gtcgtattgc
     5701 tccagcgttt gcggcaacgt caggccgagc acgtttttca catcacggtt cagtaacccc
     5761 gcgcgatcca gttcgccgag aataccgata acaccaccag cacggtgaac atcttccata
     5821 tggtatttct gggtgctcgg cgcaacttta cacagctgtg gaaccttgcg ggaaagctta
     5881 tcgatatcac tcatggtgaa gtcgatttcc gcttcctgcg ccgccgccag caggtgaagt
     5941 acggtgttag tcgatccacc catcgcgata tccagcgtca tggcgttttc aaacgccgcc
     6001 ttactggcga tattacgcgg cagtgcactt tcgtcgtttt gctcgtaata acgtttggtc
     6061 aattcaacaa tgcgtttacc agcattaagg aacagctgct tacggtccgc gtgggttgcc
     6121 agcagcgagc cgttgcctgg ctgcgacaaa cccagcgctt cggtcaggca gttcattgag
     6181 ttagcggtaa acatcccgga gcaggaaccg caggttgggc aggcggaacg ttcaacctga
     6241 tcgctctggg agtcagaaac tttcgggtct gcgccctgga tcatcgcatc aaccagatcg
     6301 agtttgatta tctgatcgga cagtttggtt ttcccggcct ccatcgggcc gccggaaaca
     6361 aagatcaccg gaatattcag gcgcagggaa gccatcagca tccccggggt gattttgtcg
     6421 cagttagaga tgcagaccat ggcgtcggcg cagtgggcgt tgaccatata ctcaacggaa
     6481 tcagcgatca gttcgcgaga tggaagtgaa taaagcatcc ccccgtggcc catggcaatc
     6541 ccatcatcca ccgcaatggt gttgaactct ttggcaacgc cgccagccgc ttcaatttgt
     6601 tcggcgacca gtttaccgag atcgcgcaga tggacgtgac ccggtacaaa ttgggtgaac
     6661 gagttcacaa ccgcgataat cggcttgcca aaatcggcgt cggtcattcc ggtggcgcgc
     6721 cacagcgcac gagcacccgc catattacga ccatgagtgg tggtggcgga acggtactta
     6781 ggcatacttt atttactccc agtgtctgtc tcgtaaatgg gacggtgcgt gccgtcccat
     6841 tttttgtatt tattgattaa cttgatctaa ccagccccat ttatcttcgg tttcgccagt
     6901 gaagaggccg aagaaggctt gctgaatgcg tttggtaacc gggccacaac ggccttcgcc
     6961 aacctgaata ccgtctacgc tgcgcactgg cgtgatttct gccgcagtac cggacataaa
     7021 cacttcatcc gccaggtaca gggattcgcg cgacagcacc tgctcacgca cttcaattcc
     7081 cagctctttc gccagtttga tgatggcatc acgggtaata cccggcagcg cggatgaggt
     7141 aaacggtggg gtgaacagca cgccgtcttt cacttcaaac aggttttcac ctgcgccttc
     7201 agagatgtaa ccgttcacat ccagcgcgat accttcctga taaccgtggc ggcgcgcttc
     7261 gctacccacc agcagggaag agaggtagtt accaccggct tttgccgccg tcgggatggt
     7321 gtttggtgct gcgcggttcc aggaggaaac catcgcatcg atcccctgct ccagcgcttc
     7381 tgcgcccaga tacgctcccc acgggaaagc agcgataatc acgtcggttg agtatcccgc
     7441 tggcgggttt actcccatgc caacatcacc gacgaagatc agcggacgga tataggcgct
     7501 ggtgagatta tttttgcgga tcacgtcacg acaagcttcc atcagctcat caatgctctg
     7561 cgaaaccggg aagcgataga ttttggcgga gtcatgcaga cgctgcatat gctcacgatg
     7621 gcggaataca accggtcctt tgtgcgagtc gtagcaacgg atgccttcaa aaaccgaagt
     7681 gccatagtgc agcgcgtgcg acatcacatg caccttcgcg tcttcccagc gaaccatctc
     7741 cccattgaac caaatgtaat cagctttctt cgtggtcatt tttatattcc ttttgcgctc
     7801 aggcgcggat ttgttgtgat gtggttgtgc tctggcagat ggcaacgtgt gcgacgtcca
     7861 ccagtttatt taactgacta aacagtaagt cgaccgaccg tgggctggca acggtcaatt
     7921 cgatatttat attttgtgca tcgctggcgg cggccatatt cattgagcag acgtggaaac
     7981 cacgatgacg caccacgcgt aaaacacgtt ctaaggtttc cggattgaag cgagccgata
     8041 cattgacctg atattgcatc atgataattt ctccaacatt tctgaattac tggcgccagg
     8101 cggcaccagc ggccagacgt tctcaagttc gtcgattgag acatgaagca ggtatggccc
     8161 atcactgttc agcatggtgt cgagtgccgc ttcaacctgg tctttacggg tgatgtgttg
     8221 gccagggatg ccgaaggcgc tggctaacat gaggaaatcg gggttatcag taagggtggt
     8281 ttcgctgtat cgttcctgaa aaaacagttg ctgccattgt cgaaccatcc ctaaccgttg
     8341 gttatcgagt aagacgattt tcaacggtaa ctgcttgcgt tttacggtgc ccagctcttg
     8401 cacattcatc atgaaagagc cgtcaccgga gatacagacg acggtatcgt tcggtcgcgc
     8461 gacttgtgcg ccaaccgccg ctggtaaacc aaaacccata gtgcctaagc cgctggaggt
     8521 gatgaaattt tccgggcgag tgtgggcgat gtgttgcgca gcccacatct ggtgctgccc
     8581 cacatctgtg gtcacgacgc aatccgcagg tttacgatcc gacagttgtt ttaacaacaa
     8641 cggcgcgtag atagcgtcgc cgggatggtc gtaacgccag gcatgttcat cacgcagctg
     8701 cgcgcagtat tgctgccagt cattgatatt taacggctgc tgtaattctg gtaacagagc
     8761 atttaaatca ccttgtaatg ccacatgtgc ctgacgcagc ttgttcattt ctgccgggtc
     8821 gatatccata tggataacgc tggcgtgtgg tgcgaaggtg ttcagtttgc cggtcacccg
     8881 gtcatcaaaa cgtgcaccca cggcgatcag caagtcgcac tcctgcaccg cgaagttcgc
     8941 cgctttggtg ccatgcattc ccagcatgcc cagatagtac ggataatctg cttcaactgc
     9001 gcccagccct ttcagcgtgc aggtggcagg catttttgtg gtagcgagaa attctcgtaa
     9061 agcaggaact gcctgcgcca tacccacgcc accaccaacg tacagcatcg gtttttgcgc
     9121 ttttgccagc atctggcgcg cttgctcaac ttcggcatgt gggaaagtca cttcgttttc
     9181 aacggtggtg aaccacggtt ccaggtcacc gctggctagc tggatatctt ttgggatatc
     9241 gaccagaacc ggaccaggac gacctgagct ggcaacgtcg aatgcttcag ccataatgcg
     9301 cggcaactct tccagcgact gcaccagaaa gctgtgcttg gtacaggcta acgacaatcc
     9361 caggacatcc acttcctgaa atgcatcagt gccgataaac ggtgcggaca cttgaccggt
     9421 gatggcaaca acagggatag aatctaacag tgcgtccgca agcccggtta tcaggttggt
     9481 tgcgcccgga ccagacgtgg cgatacatac gccagttttg ccggtagcac gggcataacc
     9541 ggtagccgcc attgccgcac cctgctcatg tcggcacagc aagtgctcca cgccgccgtc
     9601 atacaatgca tcgtaaaccg gcataattgc gccacccgga taaccgaaaa cggtgttcac
     9661 accctgtgcc cgcaacgcat gtaccaccca ctgtgcgcca ttcatagtta gttccccgtg
     9721 ctgaatgttg agaaacagaa ttttgtgctg ttattcattg tctgctcctc ggttatgttt
     9781 ttaaggtcaa aaaaaacccc cggacctttc ggtgcggggg tcttagttcg ttaaggcttg
     9841 atttctaagc ctttcctcgt ccaagtgcag ccccgcacgg tgggataata atcaccacca
     9901 cgctaatcac gaccaggcta atcactcgta gaagggctgt cattttgtct tttcttgcat
     9961 cttgttcgaa ggaatgccta aagagttacc ataggttttg taaatagtac aagatatttt
    10021 ttggccgatt tttatgatgc gcttaataat ttcaatataa gttgttgttt tttaaaaata
    10081 aaatagtgag taaaaaaatt caatttcagt atgaatgttg ttcccttcct cgtagttcat
    10141 cctttgtccg ctgcattttt gcgagcatca ttccaccgcc agaacaactc aacttcactt
    10201 gcacaaataa acaggaatca gccactaaaa ttcacgaatt agcgggctac agcagcgata
    10261 tcttatgcac ataatggtga ctcaaggagg gcttatgtca ctgtcaattg ttcatacccg
    10321 cgcagccctg ggagtaaatg cgcccccgat cactgttgag gtacatatca gtaaaggtct
    10381 acccggctta acgaaggtgg gcttaccaga aacaacggta aaagaagctc gcgatcgcgt
    10441 gcgcagcgcc attatcaata gcggatatga atatccggcg aaaaaaatca ccatcaacct
    10501 ggctccagct gatctgccaa aagaaggggg acgatatgat ttacctatcg ccattgcgtt
    10561 gctggcggcc tcagaacagc ttacagccaa taagttagat gaatatggat tagtcggaga
    10621 actggcgctt acaggcgctc tgcgtggcgt tcccggcgca atctccagta caactgaagc
    10681 tattaagtcg ggcagaaaaa ttatcgtcgc gaaagataac gaagatgaag tggggctaat
    10741 taacggtgaa ggatgcctga tagccgatca tctgcaagct gtctgtgcgt ttctggaagg
    10801 taagcacgct ctcgaacgcc cgaaaccaac tgatgcagta tcccgggcgc tacaacatga
    10861 tctcagtgat gttatcggtc aggaacaagg aaagcgagga ctggaaatta ccgccgctgg
    10921 cgggcacaac cttttactga ttgggccgcc gggaacaggt aaaacaatgc tcgccagccg
    10981 tattaatggc ctgttgccag atttaagcaa tgaagaggca ctggagagtg ctgcgatatt
    11041 aagtctggta aatgctgaat cagtacaaaa acaatggcgg cagcgcccgt tccgctcacc
    11101 tcatcacagt gcatcgttaa ctgcgatggt aggcggtggc gcaattccag ggcccggtga
    11161 aatttcgctg gcgcataacg gcgtgctttt tcttgatgag ttacctgaat ttgaaaggcg
    11221 tacactggat gccttgcgag agccgattga atccgggcag atccatcttt cacgcacgcg
    11281 agcaaaaata acctatccag cccgtttcca gcttgtcgcg gcgatgaatc ccagccctac
    11341 cggacattat cagggaaacc ataaccgctg cacaccagag caaacgctgc gttatctcaa
    11401 ccgactctct ggtccctttc tcgaccgctt cgatctctca ctggagatcc cattaccgcc
    11461 tcccggtatt ttgagtaaaa cggtagtgcc gggagaaagc agcgccaccg ttaaacaacg
    11521 cgtaatggcc gccagagagc gccaatttaa gcggcagaat aaactgaacg cctggctgga
    11581 tagtccggaa atacgccaat tctgcaaact tgagagcgaa gatgcgatgt ggctggaaga
    11641 aacgctgatc catctggggt tatcgattcg tgcctagcag cggttattga aagttgcacg
    11701 aaccattgct gatattgatc agtctgacat tatcacacgt caacatttgc aggaggcagt
    11761 tagctatcga gcgattgacc gtttgctcat ccatctgcag aaactactga cataaaaaaa
    11821 gggcatttcg ccctttttat taatcgtcag aatcggtgta gtcttcagca ccttcaacct
    11881 gcggtttacc gccggaaagg gtgtgaaaac gttttggacg cttaatacgc gtcatatact
    11941 tggaccacac gcgttctgct tctgtcactg gctcacgttc gccacggcat actgctacga
    12001 agagtttctc ttcctcggta accggctcgc gtttgccaag atccaactca ttgaaggcat
    12061 aaccatgacg ctcaagcagt tgtgcctctt tgatggtgaa atcaccatga cgagagaatc
    12121 cacgtggata atgtttattg tcgaaatatc gattagtcgt cgtaaagctt tccgccatcc
    12181 tgcacgctcc taattctttg accgagctag ttatggcgcg gagtattagt tacgcttgac
    12241 agagtgtaaa acaaaacatt taaatcataa cgacaaataa ttttgcggag agcactgtgg
    12301 atacggaatt gttaaaaact ttcctggaag ttagccgaac gcgtcacttt ggtcgagcgg
    12361 ctgaatcgct ctatctgacc cagtcagcag tgagctttcg aatcagacaa ctggaaaatc
    12421 aactgggtgt gaaccttttc acccgccaca gaaacaatat ccgtttaacc gctgccggtg
    12481 aaaaactact gccttatgca gaaacgctca tgagcacctg gcaggccgcc cgcaaggagg
    12541 tggcgcatac ctcacgacat aacgagtttt ctatcggtgc cagcgcctca ttgtgggaat
    12601 gtatgcttaa tcagtggctg ggacgtttgt atcaaaatca ggatgcccat acaggcttac
    12661 agttcgaagc gcgaattgcc caacggcagt ctctggtaaa acagctgcat gaacgccagc
    12721 ttgatcttct tattaccact gaagcgccca aaatggacga atttagtagt cagttgctgg
    12781 gatatttcac tttagcgctt tataccagtg ccccttcaaa actaaaggga gatcttaatt
    12841 atctgcgact tgagtggggg ccagattttc aacagcatga ggcaggtttg atcggtgctg
    12901 acgaagtgcc cattctgaca accagttctg ctgaactggc acagcaacag attgcgatgc
    12961 ttaatggttg cacctggcta cccgtcagct gggcgcgtaa aaaaggcggc ctgcataccg
    13021 ttgtcgatag cacaacactt tcacggccgc tttatgccat atggctgcaa aatagcgata
    13081 aaaatgcgtt gattcgcgat cttttgaaaa ttaacgtgct ggatgaagtg tattaatatg
    13141 aatggctggc aaggatgccg gtagaaggat ttacttcgga gagggttatt tcagataaaa
    13201 aaaatcctta gctttcgcta aggatgattt ctggcagggg cggagagact cgaactccca
    13261 acacccggtt ttggagaccg gtgctctacc aattgaacta cgcccctaat tag
//
LOCUS       DADLFA010000113        13132 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000113 DADLFA010000000
VERSION     DADLFA010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13132
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.113"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..184)
                     /locus_tag="JQH65_002726"
     CDS             complement(<1..184)
                     /locus_tag="JQH65_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6140094.1"
                     /translation="MKNIKLLGSAMLVSLALFSQNSLAKEYKIGASLLTQQHPFYIDL
                     ANAMKEEAKKDNVNLSV"
     gene            complement(220..1074)
                     /locus_tag="JQH65_002727"
     CDS             complement(220..1074)
                     /locus_tag="JQH65_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose bisphosphate family class II aldolase"
                     /protein_id="HAY6140095.1"
                     /translation="MYIISSKNMLKKAQSEGYAVPAFNIHNLETLQVVVESAAQLRSP
                     VMLAGTPGTYRYGGVGSLISLVQSLAREYNLPLVLHLDHHEESDDIFNKVRAGIRSVM
                     IDGSHLPFEDNIARVQEVTTFCHRFDVSVEAELGRLGGQEDDLLVDEKDSAYTSPAAA
                     AEFVQRTQIDSLAVAIGTAHGLYAHEPRLDFVRLEQIRQRVEIPLVLHGASGLSEQDV
                     QHCIRRGICKVNVATELKIAFADALKGYFYNNPAANDPRHYMQPAKAAMKEVVTRIIG
                     VCGSEGKI"
     gene            1417..2247
                     /locus_tag="JQH65_002728"
     CDS             1417..2247
                     /locus_tag="JQH65_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY6140096.1"
                     /translation="MKSVNVPRKSSNLLKRRLKIAEIVAAQGEIKVDDLSAQLGVSGV
                     TIRGDLSYLEQQGYLKRSFGGAIATPLSSEPEAIQPIAPLSLAQQMEIARHCARLIRD
                     RDTLFLGHGTICRKIIPLLSEVKKLRLITNDPEHALLANQFIDGEIILAGSEMLRPDT
                     ALAGKPLEQALQHFTITHSILEINHLDADGTLNINVPRLAAAWQLCFDHAQNKTVIVA
                     ADPAPSTAAIGHLHQAENVIVSRSINEQYQQQLQSADFVILYTSNECFTWSNRERLQE
                     "
     gene            2279..3214
                     /locus_tag="JQH65_002729"
     CDS             2279..3214
                     /locus_tag="JQH65_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAY6140097.1"
                     /translation="MGELLAEFLSRNPHQKFTQPGEFIGPFPSGAPAIFAAQVAKLSH
                     RAIFFGCVGNDDFGRLIIERLRHEGVITDGIHVMNNAVTGTAFVSYQNPQQRDFVFNI
                     PNSACGLFTAEHIDKDLLKQCNHLHIVGSSLFSFRMIDVMRKAITTIKSAGGTVSFDP
                     NIRKEMLSIPEMAQALDYLIEYTDIFIPSESELPFFARHKNLSEEQIVSDLLHGGVKH
                     VAIKRAQRGASYYKLKNGTLHAQHVTGHDIEIIDPTGAGDCFGATFITLFLSGFPAHK
                     ALQYANASGALAVMRQGPMEGISSLADIEDFLQQH"
     gene            3307..3549
                     /locus_tag="JQH65_002730"
     CDS             3307..3549
                     /locus_tag="JQH65_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HAY6140098.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLAAAYLPDVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            3539..4990
                     /gene="panF"
                     /locus_tag="JQH65_002731"
     CDS             3539..4990
                     /gene="panF"
                     /locus_tag="JQH65_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HAY6140099.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVETLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            5002..5883
                     /gene="prmA"
                     /locus_tag="JQH65_002732"
     CDS             5002..5883
                     /gene="prmA"
                     /locus_tag="JQH65_002732"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAY6140100.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            6212..7177
                     /gene="dusB"
                     /locus_tag="JQH65_002733"
     CDS             6212..7177
                     /gene="dusB"
                     /locus_tag="JQH65_002733"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAY6140101.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            7203..7499
                     /gene="fis"
                     /locus_tag="JQH65_002734"
     CDS             7203..7499
                     /gene="fis"
                     /locus_tag="JQH65_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HAY6140102.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            7585..8469
                     /gene="yhdJ"
                     /locus_tag="JQH65_002735"
     CDS             7585..8469
                     /gene="yhdJ"
                     /locus_tag="JQH65_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HAY6140103.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKIPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYDPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDII
                     LDPFAGSFTTGAVAVSSGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            8553..8732
                     /locus_tag="JQH65_002736"
     CDS             8553..8732
                     /locus_tag="JQH65_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 domain-containing protein"
                     /protein_id="HAY6140104.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(8735..9397)
                     /gene="acrS"
                     /locus_tag="JQH65_002737"
     CDS             complement(8735..9397)
                     /gene="acrS"
                     /locus_tag="JQH65_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HAY6140105.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIINGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            9796..10953
                     /gene="acrE"
                     /locus_tag="JQH65_002738"
     CDS             9796..10953
                     /gene="acrE"
                     /locus_tag="JQH65_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HAY6140106.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYNSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     GTVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMYVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            10965..>13132
                     /gene="acrF"
                     /locus_tag="JQH65_002739"
     CDS             10965..>13132
                     /gene="acrF"
                     /locus_tag="JQH65_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HAY6140107.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTNDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDT"
BASE COUNT         3319 a         3162 c         3249 g         3402 t
ORIGIN      
        1 agacacttaa attgacattg tctttttttg cctcttcttt catggcattc gcaagatcaa
       61 tatagaaagg atgttgctgt gttaataatg acgcaccaat tttatactct tttgccaacg
      121 aattttgcga gaacaatgcc aggctcacca acatagctga accaagtaat ttaatatttt
      181 tcataaacaa tcctcattgt ttttgtaggg tacaacgtct taaatttttc cttcactacc
      241 acagacacca ataatacgag ttacgacttc tttcatggcg gcctttgcgg gctgcatata
      301 gtggcgtgga tcatttgccg caggattgtt gtagaaatat ccttttaacg catcggcaaa
      361 cgcaattttc aattctgtcg cgacattcac tttacaaata ccgcggcgaa tacagtgctg
      421 aacgtcctgt tctgataagc ccgaagcacc atgcaacacc agtggaatct ccactcgctg
      481 acgaatctgt tccagccgca caaaatccag tcgtggttca tgcgcatata aaccatgtgc
      541 tgtgccgatt gctacggcca gggagtcaat ttgggtacgt tgaacaaact ctgcagctgc
      601 ggctggtgag gtataagcgg aatctttttc atccaccagc agatcgtcct cctggccgcc
      661 aagacgccct agctcggcct caacgctaac atcaaagcga tggcaaaagg tggtaacttc
      721 ttgcactcgc gcaatgttgt cttcaaatgg caaatgggag ccatcaatca tgacagaacg
      781 gatccctgcg cgaaccttgt taaaaatgtc atcactctct tcatgatggt cgagatgcag
      841 gactaacggc aggttgtact cgcgagccag cgattgtact aacgaaatta aacttcccac
      901 gccaccataa cgataagtac cgggtgttcc cgccagcata acgggagagc gaagttgtgc
      961 agcgctctca acaactacct gtaaagtttc aaggttatga atgttgaaag cggggacggc
     1021 atatccctct gactgggctt ttttcagcat gtttttgctt gaaatgatgt acatcgcagc
     1081 tcctgttatt tgtcgttcgc cttacaaaat gaagaaagag aatattcact ttcattttga
     1141 aagcaaacta gcacagcaaa agcggcaaaa agcgtgatac atgtcgaata tcatcaaaat
     1201 aacttacaaa aaaataactt aatttgtttt tcactttaca agtgaaacca ataacatctc
     1261 tgcataaata tgaataaatt catttatttt tcaatgaatt aatctatttt tgttgcgatt
     1321 tttgacccat gattttcttt tcgaattgtc gatattattg aaggataact ttcaaagaaa
     1381 aatacaaggg gctatagtgt tacgcattaa aggccaatga agagtgttaa cgtgccgaga
     1441 aaatcgtcta acctactgaa acgcagatta aaaattgcag aaattgttgc cgcccaaggt
     1501 gaaatcaaag ttgatgactt atctgcgcaa cttggtgtct ctggcgtgac gatccgcggc
     1561 gatctcagtt atctggagca acagggatat ttaaagcgat catttggcgg tgctattgcg
     1621 acaccactct cctctgaacc agaagcgata caaccgatcg cgccattgtc tctggcacaa
     1681 caaatggaaa tagcacgaca ttgcgcacgg ttaattcgcg atcgtgacac gctatttctt
     1741 ggacatggga caatctgccg taagatcatt ccattgctca gcgaagtgaa aaaactgcgt
     1801 ctgatcacca acgatcccga acatgcgctg ctggcaaatc agtttattga tggcgaaata
     1861 atactggctg gcagtgaaat gctgcgcccc gacaccgcac tggccggaaa accgcttgaa
     1921 caggcacttc agcatttcac cattacacat agcattctgg aaatcaacca tctggatgct
     1981 gatggtactt taaatatcaa cgttcccaga ctggccgctg cctggcagct ctgtttcgat
     2041 catgcccaga ataagacggt tatcgttgcc gccgatcctg cgccatcaac ggctgctatc
     2101 gggcatttgc atcaggcaga aaatgtgatc gtcagccgca gcattaacga acaatatcag
     2161 caacaattgc agtcggctga tttcgttatc ctttatacca gtaatgagtg ctttacctgg
     2221 tccaatcgcg aaaggcttca ggaatgagag gggttttgtg acaataacta tctgcgctat
     2281 gggcgaattg ctggccgagt ttttgtcccg caacccacat caaaaattca ctcagcctgg
     2341 ggagtttatc gggccatttc ccagcggtgc gccagcaatt tttgctgctc aggtggcaaa
     2401 actgtcccat cgggccatct tcttcggatg tgttggtaat gatgattttg gccgactcat
     2461 tatagagcgc ctccgtcatg aaggtgtcat taccgatggg atccatgtta tgaacaatgc
     2521 cgtcacaggt acggcgttcg tgagttatca aaatccccag cagcgcgatt tcgtctttaa
     2581 tatccctaac agcgcctgcg gcttgtttac tgctgagcac attgataagg atctgctaaa
     2641 acagtgtaac catctccata ttgtgggctc atcattgttc tcatttcgca tgatcgatgt
     2701 catgcgtaaa gcaataacga cgatcaaatc ggctggcggc accgtttctt tcgatcccaa
     2761 tattcgcaaa gagatgctga gcattcctga aatggcgcag gctctcgatt atttgattga
     2821 atatacggat attttcatcc ccagcgaaag cgaactccct ttcttcgcgc gtcacaaaaa
     2881 tctgtcagaa gaacagattg ttagcgatct tctccacggc ggcgtaaaac atgtggcgat
     2941 aaaacgcgcc cagcgtgggg ccagctatta caagcttaaa aacggtacat tacacgccca
     3001 gcatgttaca ggccacgata tcgaaattat cgatccaacg ggtgcaggcg actgctttgg
     3061 cgcaacgttt atcactcttt tcttatccgg tttcccggca cataaggcac tgcaatatgc
     3121 aaatgccagc ggcgcgctcg ccgtaatgcg gcaaggtccg atggaaggga tatcctcact
     3181 ggctgacatt gaagactttt tgcagcagca ctgaacggca attttctgtc agattatttt
     3241 ccctgcctga agagttgccg tagaattgcc cgtttctcgt atccccccat gaggaggaac
     3301 ccttccgtgg acactcgttt tgttcaggcc cataaagagg cgcgctgggc gctggggctg
     3361 acccttttgt atctggcagt ttggttagca gccgcttact tacctgacgt tgcccccggt
     3421 tttaccggct ttccgcgctg gtttgagatg gcctgcatcc tgacgccgct gctgtttatt
     3481 ggactgtgct gggcgatggt gaaatttatc tatcgcgata tcccactgga ggatgacgat
     3541 gcagcttgaa gtgattctac cgctggtcgc ctatctggtg gtggtgttcg gtatctctgt
     3601 ttatgcgatg cgtaaacgga gcaccggtac cttccttaat gagtatttcc tcggcagccg
     3661 ctctatgggc ggtattgtgc tggcgatgac gctcaccgcg acctatatca gtgcaagttc
     3721 gtttatcggc gggcctggag ctgcttataa atacggcctg ggttgggtat tgctggcgat
     3781 gattcagctt cctgcagtct ggctttcgct cggtattctc ggcaagaagt tcgcgattct
     3841 tgcgcgccgc tacaatgcag tgacgctgaa cgacatgctg tttgcccgct accagagtcg
     3901 tcttctggtg tggctggcga gtttgagttt gctggttgcg ttcgttgggg caatgaccgt
     3961 gcagtttatc ggcggtgcgc gcctgctgga aaccgcggcg ggtattcctt atgaaaccgg
     4021 gctgctgatt ttcggtatca gcattgcgtt gtataccgcc tttggcggct ttcgcgccag
     4081 cgtgctgaac gacaccatgc aagggcttgt gatgctgatt ggcaccgttg tgctgcttat
     4141 tggcgtggta catgccgcag gtggtttaag taatgcggta gagacgctgc aaaccatcga
     4201 tccgcaactg gttacgccac aaggcgctga cgatattctg tcgcctgcct ttatgacgtc
     4261 gttctgggta ctggtgtgct ttggcgtgat tggcctgccg catactgcgg tgcgctgtat
     4321 ctcttataaa gacagcaaag cggtacaccg ggggatcatc atcggtacga ttgtagtcgc
     4381 aatcctgatg ttcggtatgc acctggccgg agcgttaggt cgtgcagtga tccccgatct
     4441 caccgtaccg gatctggtga tcccgacgtt aatggtaaaa gtgctgccac cgtttgctgc
     4501 cgggatcttt ctggctgcac ctatggctgc gatcatgtcg acaattaacg cccaattgct
     4561 acaaagttcc gctacgatca ttaaagatct ctatctgaat atccgtccgg atcaaatgca
     4621 aaacgagacg cgtctgaagc ggatgtcggc ggtaattacg ttagttctcg gcgcgttgct
     4681 gctgcttgcc gcctggaagc cgccagaaat gatcatctgg ctgaatttgc tggccttcgg
     4741 tgggttggaa gccgttttcc tgtggccgct ggtgctgggt ctttactggg aacgcgccaa
     4801 cgccaaaggc gcgctaagtg cgatgatcgt tggcggcgtg ctgtatgccg tactcgcgac
     4861 gctgaatatt cagtacctgg gcttccaccc tatcgtgccc tcgttactac taagtttgct
     4921 ggctttcctg gtcggaaacc gtttcggtac atccgtcccg caagctaccg ttttgactac
     4981 tgataaataa agagttttgc catgccttgg atccaactga aactgaacac caccggcgcg
     5041 aacgctgaag atcttagcga tgcgctgatg gaagcgggtg ccgtttctat cacttttcag
     5101 gatacccacg atacgccagt atttgagccg ctgccgggcg aaacgcgcct gtggggcgac
     5161 accgatgtga ttggtctgtt cgacgctgaa accgatatga acgacgtggt ggcgattctg
     5221 gaaaaccatc cgctgctcgg cgcaggcttc gcgcataaaa tcgaacaact ggaagataaa
     5281 gactgggagc gcgaatggat ggataatttc cacccgatgc gctttggtga acgactgtgg
     5341 atctgcccaa gctggcgtga tgtgccggac gaaaacgccg tcaacgtgat gttagaccca
     5401 gggctggcgt ttggtacggg tacccatcca actacctctc tgtgcctaca atggctcgac
     5461 agcctcgatt taactggtaa aacagtcatc gactttggct gcggttccgg cattctggct
     5521 atcgcggcgc tgaaactggg tgcagcaaaa gccattggta ttgatatcga tccgcaggcg
     5581 attcaggcta gccgcgacaa cgccgaacgt aatggcgttt ctgaccgtct ggaactctac
     5641 ttaccgaaag atcagccaga agaaatgaaa gccgacgtgg tggtcgcgaa catccttgca
     5701 ggcccattac gtgaactggc accgttaatc agcgtcctgc cggtttcagg cggtttgctg
     5761 ggcctttccg gtattctggc aagccaggca gagagcgttt gtgaagctta tgccgatagc
     5821 ttcgcactgg acccggtcgt ggaaaaagaa gagtggtgcc gtattaccgg tcgtaagaat
     5881 taaccttcgc atcgccgtag ggtgacgcgg ggcgaagtgc gagcaagctc acaaaaggca
     5941 cgtaaatttg ccggttattt acgcaaattt gcgtgccaaa attttcattc ataaagaaaa
     6001 attgagaact tactcaaatt tctttgaatg taaattttag tcactatttt ctaatatgat
     6061 gatttttatg agtaattatc gcaccacgct cattttaaat gcaattcttt gatccatctc
     6121 agaggattgg tcaaagtttg gcctttcatc tcgtgcaaaa aatgcgtaat atacgccgcc
     6181 ttgcagtcac agtatggtca tttcttaact catgcgcatc ggacaatatc agctcagaaa
     6241 tcgcctgatc gcagcgccca tggctggcat tacagacaga ccttttcgga cgttgtgcta
     6301 cgagatggga gccggattga cagtatccga gatgatgtct tctaacccac aggtttggga
     6361 aagcgacaaa tctcgtttac ggatggtgca cattgatgaa cccggtattc gcaccgtgca
     6421 aattgctggt agcgatccga aagaaatggc agatgcagca cgtattaacg tggaaagcgg
     6481 tgcccagatt attgatatca atatgggttg cccggctaaa aaagtgaatc gcaagctcgc
     6541 aggttcagcc ctcttgcagt acccggatgt cgttaaatcg atccttaccg aggtcgtcaa
     6601 tgcagtggac gttcctgtta ccctgaagat tcgcactggc tgggcaccgg aacaccgtaa
     6661 ctgcgaagag attgcccaac tggctgaaga ctgtggcatt caggctctga ccattcatgg
     6721 ccgtacacgc gcctgtttgt tcaatggaga agctgagtac gacagtattc gggcagttaa
     6781 gcagaaagtt tccattccgg ttatcgcgaa tggcgacatt actgacccgc ttaaagccag
     6841 agctgtgctc gactatacag gggcggatgc cctgatgata ggccgcgcag ctcagggaag
     6901 accctggatc tttcgggaaa tccagcatta tctggacact ggggagttgc tgcccccact
     6961 gcctttggca gaggttaagc gcttgctttg cgcgcacgtt cgggaactgc atgactttta
     7021 tggtccggca aaagggtacc gaattgcacg taaacacgtt tcctggtatc tccaggaaca
     7081 cgctccaaat gaccagtttc ggcgcacatt caacgccatt gaggatgcca gcgaacagct
     7141 ggaggcgttg gaggcatact tcgaaaattt tgcgtaaaca gaaataaaga gctgacagaa
     7201 ctatgttcga acaacgcgta aattctgacg tactgaccgt ttctaccgtt aactctcagg
     7261 atcaggtaac ccaaaaaccc ctgcgtgact cggttaaaca ggcactgaag aactattttg
     7321 ctcaactgaa tggtcaggat gtgaatgacc tctatgagct ggtactggct gaagtagaac
     7381 agcccctgtt ggacatggtg atgcaataca cccgtggtaa ccagacccgt gctgcgctga
     7441 tgatgggcat caaccgtggt acgctgcgta aaaaattgaa aaaatacggc atgaactaat
     7501 tcaggttagc taaatgcttg attaaaaagg cgctactcgg catggggaag cgcctttttt
     7561 ataggtgtca caaagggagt gaccatgaga acaggatgtg aaccgacccg gtttggtaat
     7621 gaagctaaga ccattattca cggtgatgcg cttgccgaac ttaaaaagat ccccgccgaa
     7681 agtgtcgatc tgatctttgc cgacccacca tataacatcg gtaaaaattt tgatggtctg
     7741 atcgaagcct ggaaagaaga tctgtttatc gactggctgt ttgaagtgat tgcagagtgc
     7801 caccgcgttc tgaaaaagca gggcagcatg tacatcatga acagcacgga aaacatgccc
     7861 tttatcgatc tccagtgccg caagcttttt accatcaaaa gtcgcatcgt ctggtcatat
     7921 gacagttctg gagtacaggc gaaaaaacac tacggctcca tgtacgaccc catcctgatg
     7981 atggtgaaag acgcaaagaa ctacacattc aacggtgatg ctattctggt agaagccaaa
     8041 accggatcgc agcgcgcgtt gatcgattat cgcaaaaatc ctccacagcc atacaatcat
     8101 caaaaagtac cgggtaacgt ctgggatttt ccgcgcgtgc gttatttgat ggatgaatat
     8161 gagaaccacc cgacgcaaaa accggaagcc ttactgaaac gcattattct cgcctcttcc
     8221 aacccaggcg atatcattct cgacccgttt gccggtagct ttactaccgg tgccgtagcc
     8281 gtctccagcg ggcgaaaatt cattggtatt gagatcaaca gcgagtacat caaaatgggg
     8341 cttcgacggc tggatgtcgc gtcgcattac tctgcggaag aactggcgaa agtgaaaaaa
     8401 agaaagaccg gcaacctgtc aaaacgaagc cggttaagcg aagttgaccc cgatctcatt
     8461 gcaaagtaaa ggaagtgtaa gcctggtttt cagattattc atttcgtgta tatttcctgc
     8521 cagacttggt taaacatgca caggctcagg taatgattcg caagtattgg tggctcgtcg
     8581 ttttcgctgt cttcgttttt ctgtttgata ctttactgat gcagtggatt gaactgctga
     8641 caacagaaac agacaaatgc cgtaatatga actcagttaa tccactaaaa ctggttaact
     8701 gtgacgaact gaattttcag gacagaatgt gaatttacat gacacttaat tcattcgttt
     8761 gatgaattaa tttcgttatg ttttcatctg gcatgaacat tcttaatacg ttatcgacca
     8821 gcgcgggtgc ttgtttataa agatcataac ccgccatatt cattaaccag ttttgaacaa
     8881 ttccgctgaa ggcaccatta ataataatca tcacaacatc taaatcgagg ttatttgcta
     8941 cacaaccttg ttgctgacac gcctgcaata cttcgcggag agtctgcgga ttaaagccca
     9001 tcttttcgcg tatcactccc tcggccagca tctcatcatt aaattcacat ttgtgatata
     9061 agattttcag caacgcctgc tggcggggaa ttttggcaat atattgcaag ccgacaatca
     9121 atttttcacg caattgttga aacgggtcat gctctaatcc agccgtcaag tgttcctgga
     9181 ttaactcccg caatgaaggc tgttgcaacc acatctcatt aaacagttga gtcttgtttt
     9241 cgaagtgcca gtagatagcg ccacgcgtaa cgttagcggc gtcggcaatg tcgttgagcg
     9301 tcgtcttgct tacgccatgc tgcgcaaact gggcgatggc agtttcaatc agttcttgcc
     9361 gggtcttcag agcttcggct ttggttcttt ttgccatgat taattattca ggaaataaat
     9421 atattcgaca cagggtgaga aaatagcgaa ggttaatcta tcatctaatg tgtatttata
     9481 cgagagggta acattgagtt gctataaatc gtcaaataaa caatgtatat tatttaccta
     9541 aaatacattc gccacatgaa tatatatttc aatttacgag gttttaattc tgcctctttc
     9601 aacccgcgtc aaaataaaac agtagaatat taatcttttt ttgtgtttat gtgccttgag
     9661 acgccagtat tcataactat tccttacatc gacgaatgat aatttgtagg atagcaaact
     9721 gtatttttct ttctgcgagt taacgcgttg cctttctggg taaataacgc gcttttggtt
     9781 ttttgaggaa tagtaatgac gaaacatgcc aggtttttcc tcctgccctc ctttattctg
     9841 atctccgcgg ctttaatcgc cggttgtaac gataagggag aagagaaagc tcacgtcggt
     9901 gaaccgcagg ttaccgttca tattgtgaaa acggccccgt tagaagttaa gactgaatta
     9961 ccaggccgca ccaatgctta ccgtatagcc gaagttcgcc cacaggttag cgggatcgta
    10021 ctgaatcgta atttcactga aggcagcgat gtacaagcag gccagtccct gtaccagatc
    10081 gatcccgcga cctatcaggc aaattataac agcgcgaaag gcgaactggc gaaaagtgaa
    10141 gccgccgccg ccatcgcgca tttgacggta aaacgttacg ttccgctcgt gggtacgaaa
    10201 tacatcagcc agcaggagta cgaccaggcc attgctgatg ctcgccaggc cgatgccgcc
    10261 gtgattgccg caaaaggcac agtcgaaagc gctcgcatca atctcgctta taccaaagtg
    10321 actgcgccaa ttagcggacg tatcggcaaa tcgaccgtga ccgaaggcgc tcttgtcact
    10381 aatgggcaaa cgactgaact ggcgactgtc cagcagctcg atcctatcta cgttgatgtg
    10441 acccaatcca gcaacgattt tatgaggctg aagcaatccg tagagcaagg aaatttgcat
    10501 aaggaaaacg ccaccagcaa cgtagagttg gtcatggaaa acggtcaaac ctatcccctg
    10561 aaaggtacgc tgcaattctc tgatgtgacc gttgatgaaa gcaccggctc cataacccta
    10621 cgtgctgtct tccctaaccc gcaacatacg cttttgccgg gtatgtatgt gcgtgcacgg
    10681 attgatgaag gcgtccaacc tgacgccatt cttatcccgc aacaaggcgt tagccgcaca
    10741 ccgcgtggtg atgcaaccgt gctgattgtt aacgataaaa gtcaggtgga agcgcgccct
    10801 gtcgttgcca gtcaggcgat tggcgataaa tggttgatta gcgaaggtct gaaatctggc
    10861 gatcaagtca ttgtcagcgg cctgcaaaaa gcgcgtccgg gagagcaggt taaagccact
    10921 accgataccc ccgcagatac tgcatcgaag taaggtaatc tgacatggca aactttttta
    10981 ttcgacgacc gatatttgca tgggtgctgg ccattattct gatgatggcg ggcgcactgg
    11041 cgatcctaca attgcccgtc gctcagtatc caaccattgc accgcctgcg gtttctgttt
    11101 cggcaaacta tccaggtgct gatgcgcaga ccgtgcagga tacggtgacg caggttatcg
    11161 aacagaatat gaatggtatc gataacctga tgtatatgtc ctccaccaac gattccgccg
    11221 gtagcgtgac aattaccctt accttccagt ccgggaccga tcctgatatc gcgcaagtgc
    11281 aggtgcagaa caaactccag ctcgccacgc cgttgctgcc gcaggaggtt cagcagcagg
    11341 ggatcagtgt tgaaaagtcc agtagcagct atttgatggt ggcgggtttt gtctctgata
    11401 acccaggcac cacacaggac gatatctcgg actatgtggc ctctaacgtt aaagatacgc
    11461 ttagccgtct gaatggcgtc ggtgacgtac agcttttcgg cgcacagtat gcgatgcgta
    11521 tctggctgga cgccgatctg ctaaacaaat ataaactgac accggttgat gtgattaacc
    11581 agttgaaggt acagaacgat cagatcgctg ccggacagtt gggcggaacg ccagcgttac
    11641 cagggcaaca gttgaatgcc tcgattattg ctcaaacgcg gttaaaaaat ccggaagaat
    11701 tcggcaaagt gaccctgcgc gtaaacagtg acggctcggt ggtacgcctg aaagatgtcg
    11761 cacgggttga acttggcggt gaaaactata acgttatcgc tcgtatcaac ggaaaaccgg
    11821 cggcgggcct ggggattaag ctggcaaccg gcgcgaatgc tctcgatacc gcgaaagcca
    11881 ttaaggcaaa actggcggaa ttacagccat tcttcccgca gggaatgaag gttctctacc
    11941 cttatgacac cacgccattc gtccagcttt ctattcacga agtggtaaaa acgctgttcg
    12001 aagccattat gctggtgttc ctggtgatgt atctgttctt gcagaatatg cgagcaacgc
    12061 tgatccccac cattgcggta cccgtggtgt tgttagggac gtttgccatc ctcgccgctt
    12121 ttggttactc catcaacaca ctaacgatgt tcgggatggt gcttgccatc gggctgctcg
    12181 tcgatgatgc gatagtggtg gtggagaacg tcgagcgcgt gatgatggag gataagctcc
    12241 cgccaaaaga agcgacggaa aaatcgatgt cgcaaattca gggcgcactg gtgggtatcg
    12301 cgatggtgct gtcagcggta tttattccga tggcattctt cggcggttct actggggcaa
    12361 tttatcgcca gttctctatc accatcgttt cggcaatggc actttctgtt ctggtggcat
    12421 tgattcttac ccctgcgtta tgtgcaacgc tgcttaaacc cgtctctgct gagcatcacg
    12481 aaaataaggg cggtttcttc ggctggttta ataccacctt cgatcatagc gttaaccact
    12541 acaccaacag cgtcggcaaa atcctcggtt ccacaggacg atatttactg atctatgcgc
    12601 tgattgttgc aggaatggtg gtgttgtttt tacgtcttcc gtcttccttc ttacctgaag
    12661 aggatcaggg tgtctttctg accatgattc agttacccgc tggcgcgacg caagagcgga
    12721 cgcaaaaagt gttggatcaa gtgacggatt actatctgaa gaacgagaaa gcgaacgttg
    12781 aaagtgtctt tacggttaac ggctttagct tcagcggcca ggcacaaaac gccggtatgg
    12841 ccttcgtcag tctgaaaccg tgggaagagc gtaatggtga tgaaaacagt gcggaagcgg
    12901 taatccatcg tgccaaaatg gaattgggca agatccgcga cggttttgtc attccattca
    12961 atatgccagc cattgttgaa ctgggcacgg caacgggttt cgactttgag ttaattgatc
    13021 aggctgggct gggtcacgat gccctaaccc aggcccgtaa ccagttgctt ggtatggcgg
    13081 cgcaacatcc tgccagctta gtcagcgtgc gtcctaatgg cctggaagac ac
//
LOCUS       DADLFA010000114        12988 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000114 DADLFA010000000
VERSION     DADLFA010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12988
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.114"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(14..520)
                     /gene="mug"
                     /locus_tag="JQH65_002740"
     CDS             complement(14..520)
                     /gene="mug"
                     /locus_tag="JQH65_002740"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HAY6140108.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            645..720
                     /locus_tag="JQH65_002741"
     tRNA            645..720
                     /locus_tag="JQH65_002741"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:678..680,aa:Ile,seq:cat)
     gene            complement(774..1538)
                     /gene="nfeF"
                     /locus_tag="JQH65_002742"
     CDS             complement(774..1538)
                     /gene="nfeF"
                     /locus_tag="JQH65_002742"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HAY6140109.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     LFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            1826..2449
                     /locus_tag="JQH65_002743"
     CDS             1826..2449
                     /locus_tag="JQH65_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAY6140110.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(2603..4123)
                     /locus_tag="JQH65_002744"
     CDS             complement(2603..4123)
                     /locus_tag="JQH65_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HAY6140111.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSIIASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            4429..5919
                     /gene="ygjG"
                     /locus_tag="JQH65_002745"
     CDS             4429..5919
                     /gene="ygjG"
                     /locus_tag="JQH65_002745"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HAY6140112.1"
                     /translation="MITTFVFIPIFAIAAGVAQSLQYLNRYHVIREPPEHILNRLPSS
                     ASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKEHVNPGFLEYRKSVTAGGDYGA
                     VEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPVVVSAVQNQLAKQPLHSQELLD
                     PLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKLAKAYQSPRGKFTFIATSGAFH
                     GKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAMRTALNECKKTGDDVAAVILEP
                     IQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTGMGRTGKMFACEHENVQPDILC
                     LAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFGGNPLACAAALATINVLLEQNL
                     LAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMAIEFVDNEIGYNFASEMFRQRV
                     LVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALAAMRVSVEEA"
     gene            complement(5961..6293)
                     /locus_tag="JQH65_002746"
     CDS             complement(5961..6293)
                     /locus_tag="JQH65_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HAY6140113.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            6512..7495
                     /gene="ebgR"
                     /locus_tag="JQH65_002747"
     CDS             6512..7495
                     /gene="ebgR"
                     /locus_tag="JQH65_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HAY6140114.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAQEDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            7679..10771
                     /gene="ebgA"
                     /locus_tag="JQH65_002748"
     CDS             7679..10771
                     /gene="ebgA"
                     /locus_tag="JQH65_002748"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HAY6140115.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHARDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            10768..11217
                     /locus_tag="JQH65_002749"
     CDS             10768..11217
                     /locus_tag="JQH65_002749"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HAY6140116.1"
                     /translation="MRIIDNLEQFRQIYASSKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            11280..12713
                     /locus_tag="JQH65_002750"
     CDS             11280..12713
                     /locus_tag="JQH65_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY6140117.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
BASE COUNT         3131 a         3488 c         3248 g         3121 t
ORIGIN      
        1 tcggtggcgt tttttatcgc ccacgcacta ccagcgcctg gtccagctcg cgatacgctt
       61 caaccagttt ctctagtgaa acgcgactta aaccgctggg atttggcagc acccaaatct
      121 gcgtcgaacc aatggtgagc gtttgtttcc cccactgtgc accgcgctgg ctgaatccct
      181 gttcatatgc ttgtttgccc agaatcgcca acgcctgcgg ctgataatct tcaatttttt
      241 caatcagctt acgcccacct gcgtgcagct cctgctttga aacttcattg gcttgcaccg
      301 ttggacggtc taccagtttg gtgacgccac aacgataatc cagcagatgc tgtgcctcct
      361 gcggcttcaa ctgacggtcg gtaaacccgg cctgatatat caccttccag aagcgatttg
      421 ctggatgagc aaagggaaaa ccagtcccgg cggatgaaag cccagggttg ataccgcaaa
      481 acacgacccg taaccctgga gccaaaatat cctcaaccat atttactcct ttctaacatc
      541 accaggaaaa gtataacgga ttgaaaatac attgtttata aaaacagcag gcgcacggta
      601 atggctggat tgcgacacgg agttacttta taatccgcta ccatggcccc ttagctcagt
      661 ggttagagca ggcgactcat aatcgcttgg tcgctggttc aagtccagca ggggccacca
      721 gatatagcaa aggctgacga gaaatcgtca gcctttttct ttttatatct cagttacttt
      781 gcgtgccagt aagccgctgc acgtacccgc tgtgggtcat actgttccgc ttcaaagcgg
      841 cggcttaaat tcttaacgac tttaccttcg ccggttatcc agatgaagta atcatcggca
      901 gggatttgca tctgcgccag acgcgcatct accgcctgct catcatgtgc cagccattcg
      961 atattaaaac catcaaggtg cgcgagataa tcctgacagg cgttatcccg cacgctaacc
     1021 agcgcactaa cttgcggttt aacggcaagt ttgctcaacg tttccaggcg gcggcgcagt
     1081 gcgggcattc cggattcatc gcagacatac agctgatacg cgtaatcttc cggcaccacc
     1141 agcgaaccgc gcggaccagc caccgtaagt ttatcgcccg gttgcgcctg catcgcccag
     1201 ccgctggcga ccccaccgtc gtgaataaat aaatcgatcg ccagttcatg gcgtagttcg
     1261 tcatacagcg gcgtatagtc acgcgacggt gggcgtggtc cttccggcca gacgatgccc
     1321 tcttccgtta ccgttggcgg cacaaagtga gcgtcaggtt gaggaaagaa gagtttgctg
     1381 tgatcgtcaa agccgcgcga tgtaaaaccg tccagcgcct cgccgccgag gacaatgcgc
     1441 tgaaaaccgg cgctgatgcg ctcaacgcgt aacacagtca gttcacggaa gcgcagatca
     1501 ttgcgaacgc gctgcgggta gcggggggag ttattcattg ttatcgcctt cgtgatggta
     1561 atcagatata tctaaataat tttcgtaaat gataatgatt gttaatcatg ataaatgcaa
     1621 gcgattagta gaactgatat gtctatagtc tgataagacg aaccgcctct tctcaggcat
     1681 cattactcaa cgccggatgc ggcgtgaacg ccttatccgg cctacgtgtg agatgagtcc
     1741 acatttaaaa atcattttta ttcttgcaag agtgctcata tcagatataa attagatata
     1801 tctaatcaag aaaaaggagg ctgatatgag ccatcatcac gaagggtgtt gtaaacatga
     1861 aggccagcca cgccatgagg gctgctgcaa aggtgagaag tcagaacacg agcactgcgg
     1921 acacggtcac cagcatgaac acggtcaatg ctgcggtggt cgccacggtc gcggcggcgg
     1981 tcgtcggcaa cgtttctttg gtcacggtga attgcgtctg gtgattctgg atattctctc
     2041 gcgcgatgac agccacggtt acgaattgat taaagcgatt gagaatctaa cccaggggaa
     2101 ttacacccca agcccgggcg tcatctaccc aacgctggat tttctgcagg agcagtcgct
     2161 gattaccatc cgcgaagagg aaggtggtaa gaagcagatt gcgctgaccg aacaaggcgc
     2221 gcagtggctg gaagaaaacc gcgaacaggt ggagatgatt gaagaacgca tcaaagcgcg
     2281 ttgcgttggc gcggcgctgc gccagaaccc gcaaatgaag cgggcgctgg ataattttaa
     2341 agcggtgctg gatttacgcg tcaaccagag cgatatcagt gatgcacaaa taaaaaagat
     2401 cattgcggtg atcgaccgcg ccgcttttga tattacgcaa ctggattaat cgccgcatcc
     2461 gccagtggcg cggtgcaatt gccggatgcg acgcttgacg cgccttatcc ggcctacacc
     2521 cactacacac cccgcaggcc tgataagatg cgccagcatc gcatcaggca ttgtgctcca
     2581 accgccggat ccggcatacc gattaatgca gcaccgtcac cgcgtcttcc agtcggctgg
     2641 cgcggtgttt caccatcgcc gacacctgcg cactctcttc caccagctca gcatttttct
     2701 gggtgatcag gttaagctca tccactgcac gggtcaggct ggaaagccca tcggcctgtt
     2761 ccagcgttga atggctaatc tgggcgatca actgggtgac gtttttcacc tgtgccacaa
     2821 tatcttccat cgtccgtccg gcggcgtgta cctgctgcga accggattgc accttatcag
     2881 cactggcatc aatcagcttg cgaatatcgt tggcagcatt agcgctgcgg ctggctaaat
     2941 gacgcacttc ccctgccacc actgcaaaac ctttgccctg ttcgccggca cgcgccgctt
     3001 ccaccgccgc attcagggcc agaatattgg tctgaaacgc aatatcgtta atcagcgaag
     3061 taatggtgcc aatgcgctgg gtactgtcgg cgatatcgtc cattgtcttg atcaccgtgg
     3121 tcatcgcctc cccaccctgc actgccgcat tactggcgat gatagaaagt ttatcggcag
     3181 ccgacgccgt ggcagagttc tgtttcaccg acgccgccat ttggttcatg gtcgccaccg
     3241 tttgctgaac gttatcaact gtctgctggg tatgttcgtt cagttcatcg gtgcctttcg
     3301 ccagcgtctc actgccattt ctgacactgg acacctggct tgagacatcg ttaattagcc
     3361 aacggcacat caggccaagc tgccctaccg cgcgtaatgt cagccccagc tcatcgctgc
     3421 gattcaggtg ctcaacacta ttacgctctc cggtcgccac cttcagtgcc tgacgggcga
     3481 cattttctat cgggcgcaca atctgccatt caaaacaagc gcttgccaac aataccacta
     3541 acacacagag gaaatacgtc accaccgggg cagcaacaaa ccaaagcatg gccgccagca
     3601 agataaacat cagggtcatc actccacgcg cccgccagcg aagcggtaat gaaggcagtt
     3661 tacccagcca gcctttacgc accaccaggc ctttatgaat acgcttactg gtacgtccgg
     3721 cgttcagcgc tttgtacagc ggctccaccg ccgcaatctc ttcatccgtc gcccgggtac
     3781 gaatcgacat atagccactg atttttccct cgcgcaccat cggtaccgca ttggcccgca
     3841 cccaataatg gtcgccattt ttgcggcgat ttttcacgat gccgctccag ggctcccctt
     3901 ttttcagggt gaaccacata tccgcaaacg ccgcttttgg catatccggg tgacgcacca
     3961 tgttgtgcgg ctgcccttgt aactcttgca aggtaaagcc gctcacctgc acaaaagtgt
     4021 cattagcatg agtgatatag ctttgcagat cggtagtgga catcagagtg gtatcgtccg
     4081 ccagcggggt attttgctgg gtgacatacg gatgagaaga catgttcgcg tcctatgcag
     4141 gttatatggt tgttaacttc ttgtcagagt ttatgtcggc cccgctgcgg ttatctttaa
     4201 ccgattaatt tgatttagat cgcaatttgc gattaaagca caaatctaat tccttgattt
     4261 aaaatacttt cactctgtta ctatacgaaa acgttaatta tcttgcccaa aatcaggcaa
     4321 ttatcgccct gaaaacgtgc atttgcacag caatcatcaa atccataccc gacaaaaacc
     4381 gtgcaaaata acaacgaatg ttaacaaata gcattaaata ctgcgcaaat gataaccaca
     4441 tttgtgttta tcccgatttt cgcgatcgca gccggagtgg cgcaatccct gcaatactta
     4501 aatcggtatc atgtgatacg cgagcctccg gagcatattt tgaacaggtt accttcgagc
     4561 gcatcggctt tagcgtgcag cgcccacgcc ctgaatctca ttgagaagcg aacgctggat
     4621 catgaggaga tgaaagcact taaccgagag gtgattgaat acttcaaaga gcatgtcaat
     4681 ccggggtttt tagagtatcg caaatctgtt accgccggcg gggattacgg agccgtagag
     4741 tggcaagcgg gaagtttaaa tacgcttgtc gacacccagg gacaggagtt tatcgactgc
     4801 ctgggaggtt ttggaatttt caacgtgggg caccgtaatc cagttgtggt ttccgccgta
     4861 cagaatcaac ttgcgaaaca accgctgcac agccaggagc tgctcgatcc gttacgggcg
     4921 atgttggcga aaacccttgc tgcgctaacg cccggtaaac tgaaatacag cttcttctgt
     4981 aatagcggca ccgagtccgt tgaagcagcg ctgaagctgg cgaaagctta ccagtcaccg
     5041 cgcggcaagt ttacttttat tgccaccagc ggcgcgttcc acggtaaatc acttggcgcg
     5101 ctgtcggcca cggcgaaatc gaccttccgc aaaccgttta tgccgttact gccgggcttc
     5161 cgtcatgtgc cgtttggcaa tatcgaagcc atgcgcacgg ctcttaacga gtgcaaaaaa
     5221 accggtgatg atgtggctgc ggtgatcctc gaaccgattc agggtgaagg tggcgtaatt
     5281 ctgccgccgc cgggctatct caccgccgta cgtaagctat gcgatgagtt cggcgcactg
     5341 atgatcctcg atgaagtaca aacgggcatg gggcgcacgg gcaagatgtt cgcctgcgag
     5401 catgagaacg tacagccgga tatcctctgc cttgccaaag cgctcggcgg cggcgtgatg
     5461 ccgattggcg cgaccatcgc cactgaagag gtgttttcag ttctgttcga caacccattc
     5521 ctgcatacca ccacctttgg cggcaacccg ctggcctgtg cggcggcgct ggcgaccatc
     5581 aatgtgttgc tggagcagaa cttactggct caggctgagc aaaaaggcga tatgttgctg
     5641 gacggtttcc gtcaactggc gcgggaatat cccgatctgg tacaggaagc gcgtggtaaa
     5701 gggatgttga tggcaattga gtttgttgat aacgaaatcg gctataactt tgccagcgag
     5761 atgttccgcc agcgcgtact ggtggccgga acgctcaata acgccaaaac gatccgcatt
     5821 gaaccgccac tgacactgac cattgaacag tgtgaactgg tgatcaaagc ggcgcgtaag
     5881 gcgctggcgg ccatgcgagt aagtgtcgaa gaagcgtaat atcaaatcgg atggcgatac
     5941 gacgtcgcca tccgatcttt ttatacgacg cgtacgcccg caggcatcat ccgctccggc
     6001 gttaacaaca cgctttcact gccgtcatcg gtttccgcac acaacagcat gcattccgac
     6061 gtttcaccac gcattttcgc tttttgcaga ttgcataaga ccaccaccgt tttccccatc
     6121 agctcttctt cgctgtagta cggcaccagg ctggtcacgg tttgcagtgt tttttccccc
     6181 acatcaacct gtacgatgta cagcttgtcg gcgttttcat ggcgtttcac ttccacaatc
     6241 tttccgacgc gcatttccag acgtgcaaaa tcagcgtaag ccacggtttc cattgtttcc
     6301 ttccttcggg taaaatttta ctaaactata gcaaaagttt ttctcaatcc tgtaggctaa
     6361 aaatggagaa tgcaggcgtg atcacattcg tgagccgctg tgttaccgtt acagcgtcaa
     6421 agaaacgcgc tttatttact gaaaacaggt gacccgataa gtacttcctc tacaatgggg
     6481 gcgcacatca gggaaagtaa aaaaggtaaa catggcaaca ctaaaagaca tcgcaatcga
     6541 agctggcgta tccctggcga cagtatccag ggtcttaaat gacgatccga cattgaatgt
     6601 gaaagaagag acgaaacatc gcattctcga gatcgccgaa aagctggagt acaagaccag
     6661 tagtgcccgc aaactccaga caggtgcagt caaccaacac catattctgg ctatctacag
     6721 ctaccaacag gagctggaga tcaacgatcc ttactatctg gcgatccgcc acggcattga
     6781 aactcagtgc gaaaagctgg gcattgagct caccaactgt tatgaacaca gcggattacc
     6841 agacattaaa aacgtcaccg gtattttaat tgtcggcaaa cccacgcccg cccttcgcgc
     6901 cgccgcctgt gcgttgaccg acaatatctg ttttatcgac tttcacgaac ccggcagcgg
     6961 ttacgatgcg gtggatatcg atctggcacg catcagtaaa gaaatcatcg acttctatat
     7021 caatcagggc gttaatcgta ttggttttat tggcggtgaa gatgagccag gcaaagcgga
     7081 tattcgtgag gtcgcctttg cggaatatgg ccgactgaaa caagtggtac gcgaagagga
     7141 tatctggcgc ggaggctttt ccagttcgtc gggttatgaa ctggcaaaac agatgctggc
     7201 gcaggaagac tatccgaagg cactgtttgt tgcttccgat tccattgcta tcggcgtact
     7261 gcgggcaatt catgaacgag gcctgaacat cccacaggat atttcgctta tcagcgttaa
     7321 cgatatcccc accgcgcgat ttacctttcc gccgctctcc accgtgcgca tccattccga
     7381 gatgatggga agtcagggcg ttaacctggt gtatgaaaaa gcccgcgatg gtcgcgcgct
     7441 gccgctgtta gtcttcgttc ccagcaaatt aaaactgcgc ggcacgaccc gttaaatccc
     7501 cttacacact gtccggcaat cgtttttgcc ggacagtgct gccgtttatt ttcgtgatcc
     7561 agttaaagta aatgcattta tctgctactt tttagtaaaa attttactaa actccccagc
     7621 aattacacaa actaccatca ccatgaatgg ttccgatttc tctctaccgg gaggccctat
     7681 gaatcgctgg gaaaacattc agctcaccca cgaaaaccga cttgcgccgc gtgcgtactt
     7741 tttttcatat gattctgttg cgcaagcgcg tacctttgcc cgcgaaacca gcagcctgtt
     7801 tctgccctta agcggtcagt ggaatttcca cttttttgac catccgctgc aagtaccaga
     7861 agccttcacc tctgagttaa tggctgactg ggggcatatt accgtccccg ccatgtggca
     7921 aatggaaggt cacggcaaac tgcaatatac cgacgaaggt tttccgttcc ccatcgatgt
     7981 gccgtttgtc cccagcgata acccaaccgg tgcctatcaa cgtattttca ccctcagcga
     8041 cggctggcag ggtaaacaga cgctgattaa atttgacggc gtcgaaacct attttgaagt
     8101 ctatgttaac ggtcagtatg tgggtttcag caagggcagt cgcctgaccg cagagtttga
     8161 catcagcgcg atggttaaaa ccggcgacaa cctgttgtgt gtgcgcgtga tgcagtgggc
     8221 ggactctacc tacgtggaag accaggatat gtggtggtca gcggggatct tccgcgatgt
     8281 ttatctggtc ggaaaacacc taacgcatat taacgatttc actgtgcgta ccgactttga
     8341 cgaagcctat tgcgatgcca cgctttcctg cgaagtggtg ctggaaaatc tcgccgcctc
     8401 ccctgtcgtc acgacgctgg aatataccct gtttgatggc gaacgcgtgg tgcacagcag
     8461 cgccattgat catttggcaa ttgaaaaact gaccagcgcc agctttgctt ttactgtcga
     8521 acagccgcag caatggtcag cagaatcccc ttatctttac catctggtca tgacgctgaa
     8581 agacgccaac ggcaacgttc tggaagtggt gccacaacgc gttggcttcc gtgatatcaa
     8641 agtgcgcgac ggtctgttct ggatcaataa ccgttatgtg atgctgcacg gcgtcaaccg
     8701 tcacgacaac gatcatcgca aaggccgcgc cgttggaatg gatcgcgtcg agaaagatct
     8761 ccagttgatg aagcagcaca atatcaactc cgtgcgtacc gctcactacc cgaacgatcc
     8821 gcgtttttac gaactgtgtg atatctacgg cctgtttgtg atggcggaaa ccgacgtcga
     8881 atcgcacggc tttgctaatg tcggcgatat cagccgtatt accgacgatc cgcagtggga
     8941 aaaagtctac gtcgagcgca ttgttcgcca tatccacgcg cagaaaaacc atccgtcgat
     9001 catcatctgg tcgctgggca atgaatccgg ctatggctgt aacatccgcg cgatgtacca
     9061 tgcggcgaaa gcgctggatg acacgcgact ggtgcattac gaagaagatc gcgatgctga
     9121 agtggtcgat attatttcca ccatgtacac ccgcgtgccg ctgatgaatg agtttggtga
     9181 atacccgcat ccgaagccgc gcatcatctg tgaatatgct catgcgatgg ggaacggacc
     9241 gggcgggctg acggagtacc agaacgtctt ctataagcac gattgcattc agggtcatta
     9301 tgtctgggag tggtgcgacc acgggatcca ggcacaggac gaccacggca atgtctggta
     9361 taaattcggc ggcgactacg gcgactatcc caacaactat aacttctgtc ttgatggttt
     9421 gatctattcc gatcagacgc cgggaccggg cctgaaagag tacaaacagg ttatcgcgcc
     9481 ggtaaaaatc cacgcgcggg atctgactcg cggcgagttg aaagtcgaaa ataaactgtg
     9541 gtttaccacg cttgatgact acaccctgca cgcagaggtg cgcgccgaag gtgaaacgct
     9601 cgcgacgcag cagattaaac tgcgcgacgt tgcgccgaac agcgaagccc ccttgcagat
     9661 cacgctgccg cagctggacg cccgcgaagc gttcctcaac attacggtga ccaaagattc
     9721 ccgcacccgc tacagcgaag ccgggcattc tatcgccact tatcagttcc cgctgaagga
     9781 aaacaccgcg cagccagtgc ctttcgcacc aaataatgcg cgtccgctga cgctggaaga
     9841 cgatcgtttg agctgcaccg ttcgcggcta caacttcgcg atcaccttct caaaaatgag
     9901 tggcaaaccg acatcctggc aggtaaatgg cgagtcgctg ctgacccgcg agccaaagat
     9961 caacttcttc aagccgatga tcgacaacca caagcaggag tacgaagggt tgtggcagcc
    10021 gaatcatttg cagatcatgc aggaacatct gcgcgacttt gccgtagaac agagcgatgg
    10081 tgaagtgttg atcatcagcc gcacagttat tgcaccgccg gtgtttgact tcgggatgcg
    10141 ctgtacctac atctggcgca tcgctgccga tggccaggtt aacgtggcgc tttccggcga
    10201 gcgttacggc gactatccgc acatcattcc gtgcatcggt ttcaccatgg ggattaacgg
    10261 cgaatacgat caggtggcgt attacggtcg tggaccgggc gaaaactacg ccgacagcca
    10321 gcaggctaac atcatcgata tctggcgcag caccgtcgat gccatgttcg agaactatcc
    10381 cttcccgcag aacaacggca accgtcagca tgtccgctgg acggcactga ctaaccgcca
    10441 cggcaacggt ctgctggtgg ttccgcagcg cccaattaac ttcagcgcct ggcactatac
    10501 ccaggaaaac atccacgctg cccagcactg taacgagctg cagcgcagtg atgacatcac
    10561 cctgaacctc gaccaccagc tacttggcct cggctccaac tcctggggca gcgaggtgct
    10621 ggactcctgg cgcgtctggt tccgtgactt cagctacggc tttacgttgc tgccggtttc
    10681 tggcggagaa gctaccgcgc aaagtctggc gtcgtatgag ttcggcgcag ggttcttttc
    10741 cacgaatttg cacagcgaga ataagcaatg aggatcatcg ataacttaga acagttccgc
    10801 cagatttacg cctctagcaa gaagtggcaa cgctgcgttg aagcgattga aaatatcgac
    10861 aacattcagc ctggcgtcgc ccactccatc ggtgactcat tgacttaccg cgtggagaca
    10921 gactccgcga ccgatgcgct atttaccggg catcgacgct attttgaagt gcattactac
    10981 ctgcaagggc agcaaaaaat tgaatatgcg ccgaaagaga cattacaggt agtggaatat
    11041 tatcgtgatg aaactgaccg tgaatattta aaaggctgcg gagaaaccgt tgaggtccac
    11101 gaagggcaaa tcgttatttg cgatatccat gaagcgtatc ggtttatctg caataacgcg
    11161 gtcaaaaaag tggttctcaa agtcaccatc gaagatggtt atttccataa caaataacaa
    11221 ctacggcggc aaaaggagtt tgccgccacc gctaccctac acattttcgg agatgtgtta
    11281 tgtctgatac caaacgtaat acaatcggca aattcggctt gctctcgctg acttttgctg
    11341 ccgtttacag ctttaacaac gttatcaata ataatattga gcttggactg gcctcggcac
    11401 cgatgttttt cctcgcgacg attttttatt ttattccctt ctgtctgatc atcgcagaat
    11461 ttgtttcgtt aaataaaaac tcagaagccg gtgtctacgc gtgggtaaaa agttcgctgg
    11521 gcggacgttg ggcatttatt actgcctata cctactggtt cgtaaacctg ttctttttca
    11581 cctcactgtt gccgcgcgtt attgcttatg cttcgtatgc cttcctcgga tacgaatata
    11641 ttatgacgcc ggttgccacc accattatca gtatggtgct gttcgccttc tccacctggg
    11701 tttccaccaa cggggcgaaa atgttggggc caattacctc cgtcacttca acgctgatgc
    11761 tgctgttaac gctctcctac attttactgg caggtacggc gctggttggc ggcgtacagc
    11821 ctgctgaccc catcaccgtt gacgcgatga tcccgaactt caactgggcg ttcctcggcg
    11881 ttaccacctg gatctttatg gccgcaggtg gcgcggagtc cgtcgctgtg tacgttaacg
    11941 acgtcaaagg cggttcgaaa tcgttcgtta aagtgatcat cctcgccggg atttttatcg
    12001 gcgtactgta ttccgtctcc tcggtgctga ttaacgtctt cgtcagcagc aaagagttga
    12061 aatttaccgg cggatcggtg caggtattcc atggcatggc ggcgtatttt ggtctaccgg
    12121 aagcgttgat gaatcgcttt gtcggtctgg tgtcctttac cgcgatgttc ggttccctgc
    12181 tgatgtggac cgcaacgccg gtgaaaattt tcttctccga aatcccggaa ggcatctttg
    12241 gtaagaaaac cgtcgaactg aacgaaaacg gcgttccggc gcgcgcagcg tggatccagt
    12301 tcctgatcgt catcccgctg atgattatcc cgatgctcgg ttccaatacc gtgcaggatc
    12361 tgatgaatac tattattaat atgaccgccg cagcgtccat gcttccgccg ttattcatca
    12421 tgctggctta cctgaattta cgcgccaaat tagatcacct gccacgcgat ttccgtatgg
    12481 gctcccgccg caccggtatt atcgttgttt caatgctgat tgcgatattt gccgtagggt
    12541 ttgtcgcttc gacattcccg actggcgcga atattctgac catcattttt tataacgtcg
    12601 gcggtattgt tatcttcctt ggctttgcgt ggtggaaata cagtaaatat ataaagggat
    12661 taacggctga agagcgccat attgaagcga cgccagccag caatgttgat taagcagaat
    12721 aataaaaaat gagcaggcat tttccctctt cctggatgag gagagggaaa atagtttctg
    12781 cctttatatt tttataatta caacgataaa aggctgtact ttttctttag ctcatggatt
    12841 aacacaatga aattaatcac tgcaccatgc agagcattac ttgctctgcc gttttgctac
    12901 gccttttctg cggcaggaga agaagcacgt ccggcagaac atgacgacac aaaaacaccc
    12961 gcaattacct cgacatcttc tccttcat
//
LOCUS       DADLFA010000115        12914 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000115 DADLFA010000000
VERSION     DADLFA010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12914
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.115"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..880)
                     /gene="rarD"
                     /locus_tag="JQH65_002751"
     CDS             complement(<1..880)
                     /gene="rarD"
                     /locus_tag="JQH65_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HAY6140118.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMLLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRR"
     gene            complement(932..1399)
                     /locus_tag="JQH65_002752"
     CDS             complement(932..1399)
                     /locus_tag="JQH65_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HAY6140119.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1564..2433
                     /gene="pldA"
                     /locus_tag="JQH65_002753"
     CDS             1564..2433
                     /gene="pldA"
                     /locus_tag="JQH65_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HAY6140120.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2566..4395
                     /gene="recQ"
                     /locus_tag="JQH65_002754"
     CDS             2566..4395
                     /gene="recQ"
                     /locus_tag="JQH65_002754"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAY6140121.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMCKLERFGKPFMALIRAHVDGDDEE"
     gene            4459..5079
                     /gene="rhtC"
                     /locus_tag="JQH65_002755"
     CDS             4459..5079
                     /gene="rhtC"
                     /locus_tag="JQH65_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HAY6140122.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQIELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5141..5761)
                     /gene="rhtB"
                     /locus_tag="JQH65_002756"
     CDS             complement(5141..5761)
                     /gene="rhtB"
                     /locus_tag="JQH65_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HAY6140123.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            5872..6894
                     /gene="pldB"
                     /locus_tag="JQH65_002757"
     CDS             5872..6894
                     /gene="pldB"
                     /locus_tag="JQH65_002757"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HAY6140124.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            6902..7702
                     /gene="yigL"
                     /locus_tag="JQH65_002758"
     CDS             6902..7702
                     /gene="yigL"
                     /locus_tag="JQH65_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HAY6140125.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            7778..8677
                     /gene="bioP"
                     /locus_tag="JQH65_002759"
     CDS             7778..8677
                     /gene="bioP"
                     /locus_tag="JQH65_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HAY6140126.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMNKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTLITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8565..9518)
                     /gene="metR"
                     /locus_tag="JQH65_002760"
     CDS             complement(8565..9518)
                     /gene="metR"
                     /locus_tag="JQH65_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HAY6140127.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9754..12015
                     /gene="metE"
                     /locus_tag="JQH65_002761"
     CDS             9754..12015
                     /gene="metE"
                     /locus_tag="JQH65_002761"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAY6140128.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPARHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTWLWLGKVKGEQFDRLSLLNDILPVYQQVLAELEKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDGVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12300..12545)
                     /locus_tag="JQH65_002762"
     CDS             complement(12300..12545)
                     /locus_tag="JQH65_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAY6140129.1"
                     /translation="MITETEINVLKVMAEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVTEKGYNFVKSENK"
     gene            complement(12535..>12914)
                     /locus_tag="JQH65_002763"
     CDS             complement(12535..>12914)
                     /locus_tag="JQH65_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6140130.1"
                     /translation="FNPDLAGGPIENLTTDGVTINREGIAIVEKHIARFGHDPINEVM
                     INRLKDIEKGKIPPEQVDLNFYTHECREYQRYCNLGWETGQPDGDAGYALWNHTHTAT
                     LEDYKLKGELNDLYHQDALDYDN"
BASE COUNT         3051 a         3261 c         3442 g         3160 t
ORIGIN      
        1 ttctacgctg cgtataaatc gcatccatca caaaaattgc cagcgccacc caaataaagg
       61 cgaaggtcac catcttatcg gcacccggtt tttcaccata aaacgtcact gccaacagga
      121 acatcagcgt cgggccaata tactggaaga agcctaacgt ggagagacgc aggcgtgtag
      181 cagctgcggt aaaacacaac agcggtacgg tggtgacaat accggcggcg atcagcagca
      241 aattcagcaa catcgggttt tgccccatat ggctggttga gctgtcggca atagcaaaca
      301 gataaatcgc cgccacgggc agcagccaca tggtttcgat taacatgccg gtttgcgctt
      361 caacggcaat tttcttgcgt accagtccgt agaaggcaaa actaaacgcc agccccagcg
      421 cgatgattgg cagcgaacca aaagtccaca actgaaccaa cacgccacat atcgccagaa
      481 tcaccgccag ccattgcatc cggcggaatc gctcgccgag gaaaatcatc cccagcacaa
      541 tgttcaccag cgggttaata aagtaaccaa ggctcgcttc cagcatatgg tgattgttca
      601 ccgcccagat aaacagtagc cagttgccac caatcagcac ggcagagact gccagcataa
      661 aaattttctg tggcgtctga atcagcgttt ttaaatagga ccactggcgg caaatgctca
      721 tcagcaccac cataaagaaa aacgaccaga tcacgcgatg cgtcaggatc tcatcggcgg
      781 gcacgtagta aatcagcttg aagtatgctg gcgcaatacc ccaaataaaa taagcggcaa
      841 gagcgagtaa tacgccctgc cgcgtttgtt ttgcatccat cgggaatact catttttaat
      901 tggtaacagc agtttacctg cttttatgtc ttcaacctac catataggtg gcggtggcac
      961 tggcaatata aagctgttct tcattgtgta attcaacgcg ggcgacggcg actttattgc
     1021 ctgcacgcaa caggctactg gtagcagtaa aacgctcgcc cctgcctggg cgcagataat
     1081 caacgcgaag atcaatggtc cccatccgcg atagccgctg gcgtagttca tcttcactga
     1141 tggtttcgtg gcgggttaag gtacttccca cgcacaccag accggcggcg acatccagcg
     1201 ccgacgcaat gactccgccg tgcaaaatgc tttgcgccca gttacccacc atcatcggct
     1261 gatttttaaa ggccagctgt gcgaactctt tttcgtaacg ctccagctcc atccctaacg
     1321 cgcggttaaa tggcatgtga taaacaaaca tctcacccac taatttcagg gcttgttcag
     1381 cggtcagtac ggcagacata tcatccttac acttcattgg ttaatgaaat gttgatttta
     1441 tgcttctttg ttgttggttt ctactttagg aagggataac taacggcaat ggagttaagt
     1501 atgtaaaata gcccgcagaa aaacattcac cttatcaata attcgttacg gagaacacga
     1561 ccgatgcgga ctctgcaggg ctggttgttg ccggtgttta tgttgcctat ggcagtatat
     1621 gcacaagagg caacggtgaa agaggtgcat gacgcgccag cggtgcgtgg cagtattatc
     1681 gccaatatgc tgcaggagca tgacaatccg ttcacgctct atccttatga caccaactac
     1741 ctcatctaca cccaaaccag cgatctgaac aaagaagcga ttgccagtta cgactgggcg
     1801 gaaaatgcgc gtaaggatga agtaaagttt cagttgagcc tggcatttcc actgtggcgt
     1861 gggattttag gcccgaactc ggtgttgggt gcgtcttata cgcaaaaatc ctggtggcaa
     1921 ctgtccaata gcgaagagtc ttcaccgttt cgtgaaacca actacgaacc gcaattgttc
     1981 ctcggttttg ccaccgatta ccgttttgca ggttggacgc tgcgcgatgt ggagatgggg
     2041 tataaccacg actctaacgg gcgttccgac ccgacctccc gcagctggaa ccgcctttat
     2101 actcgcctga tggcagaaaa cggtaactgg ctggtagaag tgaagccgtg gtatgtggtg
     2161 ggtaatactg acgataaccc ggatatcacc aaatatatgg gttactacca gcttaaaatc
     2221 ggctatcacc tcggtgatgc ggtgttgagt gcgaaaggac agtacaactg gaataccggt
     2281 tacggcggcg cggagttagg cttaagttac ccgatcacca aacatgtgcg cctttatact
     2341 caggtttaca gcggctatgg cgaatcgctc atcgactata acttcaacca gacccgtgtc
     2401 ggtgtaggag ttatgctaaa cgacttgttt tgatgaacgg ttgagtggtt ggcaaatctg
     2461 gaacccagca tccaggatta ccctctcaga gactaaaagc attgcagttt ctcgcgcagg
     2521 cgctgaaaat agcgcctgtt tttatttcag gcaatcgggg tgaatgtggc gcaggcggaa
     2581 gtgttgaatc tggagtccgg agctaaacag gttttacaag aaacctttgg ctaccaacag
     2641 tttcgccccg gccaggaaga aattatcgac actgtgcttt ccggtcgcga ttgcctggtc
     2701 gtcatgccca ccggtggcgg aaaatccctt tgctatcaaa ttcctgcctt attgctaaac
     2761 ggccttaccg tggttgtttc accgctgatt tcgttgatga aagatcaggt ggatcaactg
     2821 caagccaacg gcgtggcggc ggcgtgcctt aactcgacgc aaacccgcga gcagcaactt
     2881 gaagtgatga caggctgccg caccgggcaa attcgtctgc tttatatcgc cccggaacgc
     2941 ctgatgctgg ataactttct tgagcatctg gcgcactgga atccggtgtt attagccgtt
     3001 gatgaagcgc actgtatctc ccaatggggc cacgatttcc gcccggaata tgccgcgctt
     3061 ggtcagttac gccagcgttt cccgaccctg ccgtttatgg cgctgactgc cacagccgac
     3121 gacaccacgc gccaggatat cgtgcgcttg ctggggctga acgatccgct gattcaaatc
     3181 agcagttttg accgtccgaa tattcgctac atgctgatgg agaagttcaa accgctcgat
     3241 cagttgatgc gctacgtgca ggaacagcgc ggtaagtcag gcattatcta ctgcaacagc
     3301 cgcgcgaaag tagaagacac cgctgcgcgc ctgcaaagca agggaattag cgcggcggcc
     3361 tatcatgctg ggctggaaaa taatgttcgc gccgacgtgc aggaaaaatt tcagcgcgat
     3421 gacctgcaaa ttgtggtggc gacggtggcg ttcggcatgg gcatcaataa accaaacgtt
     3481 cgcttcgtgg tccactttga tattccgcgc aatatcgaat cctattatca ggaaaccggt
     3541 cgcgccgggc gtgatggcct gcccgcggaa gcgatgctgt tttacgatcc ggccgatatg
     3601 gcgtggctgc gccgttgcct ggaagagaag ccgcaggggc agttacagga tatcgaacgt
     3661 cataaactca atgcgatggg cgcttttgcc gaagcgcaaa cttgccgtcg tctggtattg
     3721 ctgaactatt ttggcgaagg gcggcaagag ccgtgcggga actgcgatat ctgcctcgat
     3781 ccgccgaaac agtacgacgg ttcaaccgat gctcagattg ccctttccac cattggtcgt
     3841 gtgaatcagc ggtttgggat gggttatgtg gtggaagtga ttcgtggtgc taataaccag
     3901 cgtatccgcg actatggtca tgacaaactg aaagtctatg gcatgggccg tgataaaagc
     3961 catgaacatt gggtgagcgt gatccgccag ctgattcacc tcggcctggt gacgcaaaat
     4021 attgcccagc attctgccct acaactgaca gaggccgcgc gcccggtgct gcgcggcgaa
     4081 tcctctttgc aacttgccgt gccgcgtatc gtggcgctca aaccgaaagc gatgcagaaa
     4141 tcgttcggcg gcaactatga tcgcaaactg ttcgccaaat tacgcaaact gcgtaaatcg
     4201 attgccgatg aaagtaatgt cccgccgtac gtggtgttta acgacgcaac cttgattgag
     4261 atggctgaac agatgccgat caccgccagc gaaatgctca gcgttaacgg cgttgggatg
     4321 tgcaagctgg aacgctttgg taaaccgttt atggcgctga ttcgtgcgca tgtcgatggc
     4381 gacgacgaag agtagtcagc agcataaaaa agtgccagga tgaagactcc gtaaacgttt
     4441 cccccgcgag tcaaatgtat gttgatgtta tttctcaccg tcgccatggt gcacattgtg
     4501 gcgcttatga gccccggtcc cgatttcttt tttgtctctc agaccgctgt cagtcgttcc
     4561 cgtaaagaag cgatgatggg cgtgctgggt attacctgcg gcgttatggt ttgggcaggg
     4621 attgcgttgc ttggcctgca tttgattatc gaaaaaatgg cctggctgca tacgctgatt
     4681 atggttggcg gtggcctgta tctctgctgg atgggttacc agatgctacg cggtgcactg
     4741 aaaaaagagg ctgtatctgc gcctgcacca cagattgagc tggcgaaaag cgggcgcagt
     4801 ttcctgaaag gtttactgac caatctcgct aatccgaaag cgattatcta ctttggctcg
     4861 gtgttctcat tgtttgtcgg tgataacgtt ggcactaccg cgcgctgggg catttttgcg
     4921 ctgatcattg tcgaaacact ggcgtggttt accgtcgttg ccagcctgtt tgccctgccg
     4981 caaatgcgcc gtggttatca acgtctggcg aagtggattg atggttttgc cggggcgtta
     5041 tttgccggat ttggcattca tttgattatt tcgcggtgat gccagacgcg tcttcagagt
     5101 aagtcggata aggcgtttac gccgcatccg acattatttt tcacgcatgc ctcgccgatg
     5161 ctaacagcgc tcccaccagc ataaacaacg agccgaaaat cttattcagc gccttcatct
     5221 gctttggtcc tttaatccat agagcaatcc gttgagcaag ggtggcgtaa ccgatcatca
     5281 caataatatc gactacaata gtggtgacgc cgagcacgat atactgcatc agttgcggct
     5341 gctgtggcat gatgaattgc ggaaatagcg ccgccagaaa cacaatactt ttgggattgg
     5401 tgagattcac aaaaactgcg cgctggaaca aatgtcgacg cgattgagta gaggccagcg
     5461 atttaaggtc aattgcacca gcggcgcgcc actgctggat tcccagccaa atcaagtaag
     5521 ccgcgcctgc ccacttcaac acttcaaacg caatcactga gcgggaaaat agcgtcccca
     5581 aacccacgcc aaccagcaca atatgaatcg ccagtccggt ctgaagccca gcaatagacg
     5641 ccaccgcgcc gcgataaccg tggttgagcg aggtggtcat agtgttgatt gcaccagagc
     5701 ctggcgacag gcttaaaatg atcgatgtca gcaggtaggc aaaccaccat tctaaggtca
     5761 tgatgaactc ccggtgtgtc tatttttgtg ccacaatacg ctactgtcgc agcgttgtgt
     5821 caggcacgct aaaaaaaacg attttacgtg gtttaagagg cagattaccc gatgtttcag
     5881 cagcaaaaag actgggaaac aagagaaaac gcgtttgctg cttttaccat gggaccgctg
     5941 actgatttct ggcgtcagcg tgatgaagca gagtttactg gtgtggatga cattccggtg
     6001 cgctttgtcc gttttcgcgc acagcaccat gaccgggtgg tagtcatctg cccggggcgt
     6061 attgagagct acgtaaaata tgcggaactg gcctatgacc tgttccattt ggggtttgat
     6121 gtcttaatca tcgaccatcg cgggcaggga cgttccggtc gcctgttagc cgatccgcat
     6181 ctcgggcatg ttaatcgctt taatgattat gttgatgatc tggcggcatt ctggcagcag
     6241 gaggtccagc ccggtccgtg gcgtaaacgc tatatactgg cacattcgat gggcggtgcg
     6301 atctccacat tatttctgca acgccatcca ggtgtatgtg acgccattgc gctaactgcg
     6361 ccaatgtttg ggatcgtgat tcgtatgccg tcatttatgg cacggcagat cctcaactgg
     6421 gccgaagcgc atccacgttt ccgtgatggc tatgcaatag gcaccgggcg ctggcgcgcg
     6481 ttgccgtttg ctatcaacgt actgacccac agcagacagc gatatcgacg taacttacgc
     6541 ttctatgctg atgacccaac gattcgcgtc ggtgggccga cctaccattg ggtacgcgaa
     6601 agtattctgg ctggcgaaca ggtgttagcc ggtgcgggtg atgacgccac gccaacgctt
     6661 ctcttgcagg ctgaagagga acgcgtggtg gataaccgca tgcatgaccg tttttgtgaa
     6721 ctccgcaccg ccgcgggcca tcctgtcgaa ggaggacggc cgttggtaat taaaggtgct
     6781 taccatgaga tcctttttga aaaggacgca atgcgctcag tcgcgctcca cgccatcgtt
     6841 gattttttca acaggcataa ctcacccagc ggaaaccgct ctacagaggt ttaaatttct
     6901 tatgtaccag gttgttgcgt ctgatttaga tggcacgtta ctttctcccg accatacgtt
     6961 atccccttac gccaaagaaa ctctgaagct gctcaccgcg cgcggcatca actttgtgtt
     7021 tgcgaccggt cgtcaccacg ttgatgtggg gcaaattcgc gataatctgg agattaagtc
     7081 ttacatgatt acctccaatg gtgcgcgcgt tcacgatctg gatggtaatc tgatttttgc
     7141 tcataacctg gatcgcgaca ttgccagcga tctgtttggc gtagtcaacg acaatccgga
     7201 catcattact aacgtttatc gcgacgacga atggtttatg aatcgccatc gcccggaaga
     7261 gatgcgcttt tttaaagaag cggtgttcaa atatgcgctg tatgagcctg gattactgga
     7321 gccggaaggc gtcagcaaag tgttcttcac ctgcgattcc catgaacaac tgctgccgct
     7381 ggagcaggcg attaacgctc gttggggcga tcgcgtcaac gtcagtttct ctaccttaac
     7441 ctgtctggaa gtgatggcgg gcggcgtttc aaaaggccat gcgctggaag cggtggcgaa
     7501 gaaactgggc tacagcctga aggattgtat tgcgtttggt gacgggatga acgacgccga
     7561 aatgctgtcg atggcgggga aaggctgcat tatgggcagt gcgcaccagc gtctgaaaga
     7621 ccttcatccc gagctggaag tgattggtac taatgccgac gacgcggtgc cgcattatct
     7681 gcgtaaactc tatttatcgt aatcgttctt tatttggtca gttgtcaacc tgatacttcg
     7741 ctacaatgga tactcgttaa tcaaagagtt ttccattgtg gcgctactta tcatcaccac
     7801 gattctgtgg gccttctcct ttagctttta tggcgagtac cttgcggggc acgtcgatag
     7861 ctattttgcg gtgctggtgc gcgttggcct ggcggcactc gtttttctgc cgtttctgcg
     7921 tacccgtggc aatagcctga aaacagtcgg cctgtatatg ctggtgggcg cgatgcagct
     7981 cggcgtgatg tatatgctga gtttccgcgc ttatctctat ctgacggttt ccgaactgct
     8041 gctgttcacc gtactgacgc cgctctacat cacgctgatt tatgacatca tgaataagcg
     8101 ccgtctgcgc tggggatatg cttttagtgc cttgttggcg gtgattggtg ccgggattat
     8161 tcgctatgat caggtcaccg accatttctg gactggcttg ttgctggtgc aactctccaa
     8221 tatcactttt gccatcggca tggtgggtta caaacgcctg atggaaactc gcccgatgcc
     8281 gcagcataac gcctttgcgt ggttctatct tggcgcgttc ctggtggcgg taattgcatg
     8341 gttcttgctg ggaaatgcgc agaaaatgcc gcaaaccacg ttgcaatggg gcattctggt
     8401 gtttcttggc gtggtggctt ccgggattgg ctactttatg tggaactacg gcgcgacgca
     8461 ggtggacgcc ggaacgctgg gaattatgaa taatatgcac gttccggcag ggctgctggt
     8521 aaacctggct atctggcacc aacagccgca ctggccaacg ttgattacag gcgcgctggt
     8581 gatcctggcc tcactgtggg tgcatcgtaa gtgggtcgct ccgcgctctt cacaaacggc
     8641 agatgatcgc aggcgtgatt gcgcgctgag cgaataaacg cttccgtaac tggctgacgc
     8701 tgctcgccat cgcgcacggc ggcgtacagt cggctccaca agccttcgcc cagggttttg
     8761 gtcaccacca gaccctggcg ctcaaaactc tctactaccc aatgcggtag cgcggcaata
     8821 cccatccgcg cggcaaccat ctgaatcaac aataaggtgt tatcgacgct tttcagtgac
     8881 ggactgacgc ctgccggctg aagaaaatgc cgccagacat ccagtcgact acgctgcacc
     8941 ggataaatta atagcgtctc gctggcgaga tcttccggtg taatacgcgt ttttgccgcc
     9001 agtggatggt caggtgctaa caccagacgc acttcatagt cgaacatcgg cgaataatgc
     9061 agaccactgc gcggcagaat atcggacgtc attaccagat ccagctctcc ctgttgcaag
     9121 gcgggctgcg ggtcaaatgt cacgcccgat ttaaagtcca tctcgacctg cggccagttc
     9181 ttatggaaat tttctaacgc gggcgtcagc cactgtatac agctatggca ctcaatggca
     9241 atgcgcagac gcgtctgctg cggttcattg caggcttgca gtgcctggct aatttgtggc
     9301 agcacctggt ttgccagttg caacaggatt tctccctgcg gtgtaaagcg aagcggctgg
     9361 cttttacgca caaatagccg gaagccaagg cgttgttcca gatcgctaaa ctggtgagac
     9421 agggcggatt gcgtttgatg caacgtcgcc gcagcggctg cgagtgagcc gcagttccgc
     9481 aacgcttgta gcgttttcag atgttttact tcgatcatga aagtccttca cttcggcatg
     9541 aataatttgc gcttgaggaa tatacagtaa ccgccaatta tggatgtgta aacatctgga
     9601 cggctaaaat ccttcgtctt ttaaatttat ggtgcgttgg ctgcgtttct ccaccccggt
     9661 cacttacttc agtaagctcc cggggatgaa taaacttgcc gccttcccta aattcaaaat
     9721 ccataggatt tacatataat tagaggaaga aaaatgacaa tattgaatca caccctcggt
     9781 ttccctcgcg ttggcctgcg tcgcgagctg aaaaaagcgc aagagagtta ttgggcaggg
     9841 aactccacgc gtgaagaact gctggcggta gggcgtgaat tgcgtgctcg tcactgggat
     9901 caacaaaagc aagcgggtat cgacctgctg ccggtgggcg attttgcctg gtacgatcat
     9961 gtactgacca ccagtctgct gctgggtaac gttccggcgc gtcatcagaa caaagatggt
    10021 tcggtagata ttgacaccct gttccgtatt ggtcgtggac gtgcgccgac tggcgaacct
    10081 gcggcggcag cggaaatgac caaatggttt aacaccaact atcactacat ggtgccggaa
    10141 ttcgttaaag gccaacagtt caaactgacc tggacgcagc tgctggagga agtggacgag
    10201 gcgctggcgc tgggccacaa cgttaagcct gtgctgctgg ggccggttac ctggctgtgg
    10261 ctggggaaag tgaaaggtga acagtttgac cgcctgagcc tgctgaacga cattctgccg
    10321 gtttatcagc aagtgctggc agaactggag aaacgcggca tcgagtgggt acagattgat
    10381 gaacccgcgc tggtactgga actaccacag gcgtggctgg acgcatacaa acccgcttac
    10441 gacgcgctcc agggacaggt gaaactgctg ctgactacct attttgaagg cgttacgcca
    10501 aatctcgaca cgattactgc gctgcctgtt cagggactgc atgtggacct tgtacatggt
    10561 aaagatggcg ttgctgaact gcacaagcgc ctgccttctg actggctgct gtctgcggga
    10621 ctgatcaatg gtcgtaacgt ctggcgcgcc gatcttactg agaaatatgc gcaaattaag
    10681 gacattgtcg gcaaacgtga tttgtgggtg gcatcttcct gctcgttgct gcacagcccc
    10741 atcgacctga gcgtggaaac gcgtcttgat gcagaagtga aaagctggtt tgccttcgcc
    10801 ttacaaaaat gccatgaact ggcactgctg cgcgatgcgc tgaacagtgg tgacacggca
    10861 gctctggcag agtggagcgc cccaattcag gcgcgtcgtc actctactcg tgtacataat
    10921 ccggcggtag aaaagcgtct ggcggcgatc accgcccagg acagccagcg tgcgaatgtc
    10981 tatgaagtgc gtgctgaagc ccagcgtgcg cgttttaaac tgccagcgtg gccgaccacc
    11041 acgattggtt ccttcccgca aaccacggaa attcgtaccc tgcgtctgga tttcaaaaag
    11101 ggcaatctcg acgccaacaa ctaccgcacg ggcattgcgg aacatatcaa gcaggccatt
    11161 gttgagcagg aacgtttggg actggatgtg ctggtacatg gcgaggccga gcgtaatgac
    11221 atggtggaat actttggcga gcacctcgac ggatttgtct ttacgcaaaa cggttgggta
    11281 cagagctacg gttcccgctg cgtgaagcca ccgattgtta ttggtgacgt tagtcgcccg
    11341 gcaccgatta ccgtggagtg ggcgaagtat gcgcaatctc tgactgataa accggtgaaa
    11401 gggatgctga ctggcccggt gaccattctc tgctggtcgt tcccgcgtga agatgtcagc
    11461 cgtgaaacca tcgccaaaca aattgcgctg gcgctgcgtg atgaagtggc ggacctggaa
    11521 gccgcaggaa ttggcatcat ccagattgat gaaccggcgc tgcgcgaagg tttaccgctg
    11581 cgtcgtagcg actgggatgc atatctccag tggggcgtgg aggccttccg tatcaacgcc
    11641 gccgtggcga aagatgacac acaaatccac actcacatgt gttattgcga gttcaacgac
    11701 atcatggatt ccattgcggc actggatgca gacgtcatca ccatcgaaac ctcgcgttcc
    11761 gacatggagt tgctggagtc gtttgaagag tttgattatc cgaatgaaat cggtccaggc
    11821 gtctatgaca ttcactcgcc aaacgtaccg agcgtagaat ggattgaagc cttgctgaag
    11881 aaagcggcaa aacgcattcc ggcagagcgc ctgtgggtca acccggactg tggcctgaaa
    11941 acgcgcggct ggccagaaac ccgcgcggca ctggcgaaca tggtgcaggc ggcgcagaat
    12001 ttgcgtcggg gatgatgaat attaacgagt cactgtaaat tgcagtacga tgaaagaggc
    12061 aaaactgcct gataccaggc ctacataatt tatgcaattt attgaatttt tgagttttgt
    12121 tggccggaca aggcgttcac gccgcatccg gcatgaacaa cgcgcacttt gccaataatc
    12181 tgatggcttc catcaccgga agccattttg ctatctgcgc ccgttcagat acagaaataa
    12241 ccgaatcatt tttacgcaaa atataatcct gagcgatttg ttcagtaatc aacgctacgt
    12301 tatttattct cacttttcac gaaattgtat cccttttcag taacgtggta gtgaggcata
    12361 gacttatgtc cgctatcctc aataataacc aaaccctcgt tagctaattt gttaacaatg
    12421 ttaacaacat taccgaatcc tggaatttgc cttatggata gtgtgcgatc gagattcatc
    12481 cagttccagt ttatatcttt ctcagccata actttcagta cgttaatttc agtttcagtt
    12541 atcatagtca agtgcatcct ggtggtataa gtcattaagt tctcccttaa gtttataatc
    12601 ttctagagta gcagtatgcg tatgattcca gagtgcatag cctgcgtcac catcaggttg
    12661 tcctgtttcc caaccaagat tacagtaacg ctgatattcc cgacactcat gagtatagaa
    12721 gttaagatct acttgttctg gggggatttt tcctttctca atgtctttca gtctattgat
    12781 catcacttca tttatagggt catgaccaaa tctggctatg tgtttttcaa caatagcgat
    12841 accttccctg ttgattgtta cgccatctgt cgtcagattt tctattggac caccggcaag
    12901 gtcgggatta aatg
//
LOCUS       DADLFA010000116        12908 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000116 DADLFA010000000
VERSION     DADLFA010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12908
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.116"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            13..1566
                     /locus_tag="JQH65_002764"
     CDS             13..1566
                     /locus_tag="JQH65_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HAY6140131.1"
                     /translation="MSEFARCHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPEDL
                     CTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKIS
                     KDIESGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDEWENL
                     HALKEKRLNGMPKTTPKSGQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSIT
                     PPLKNYNERLHNTNLFNPKYQKKAVMNYMEYLVTCQLDEQQMSSLLYERDDLSAIDFS
                     LLMKTIKAFSFGGDLQTLASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNHQ
                     QDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWLNKGCSTYQQL
                     YNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVEG
                     TWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPGKVFTLRGGTLNINIPGRSALLLGKT
                     GEPPNYLYL"
     gene            1902..2879
                     /gene="csiD"
                     /locus_tag="JQH65_002765"
     CDS             1902..2879
                     /gene="csiD"
                     /locus_tag="JQH65_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HAY6140132.1"
                     /translation="MNALIAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKPLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            2899..4167
                     /gene="lhgO"
                     /locus_tag="JQH65_002766"
     CDS             2899..4167
                     /gene="lhgO"
                     /locus_tag="JQH65_002766"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HAY6140133.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNDIRYDNCGKMLIATSDLEMERM
                     RALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVVRTRQGGEYEASTLISCSGLMANRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRATRSVDALHAAFNQ"
     gene            4190..5625
                     /gene="gabD"
                     /locus_tag="JQH65_002767"
                     /pseudo
     CDS             4190..5625
                     /gene="gabD"
                     /locus_tag="JQH65_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            5639..6905
                     /gene="gabT"
                     /locus_tag="JQH65_002768"
                     /pseudo
     CDS             5639..6905
                     /gene="gabT"
                     /locus_tag="JQH65_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417148.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
     gene            7143..8504
                     /gene="gabP"
                     /locus_tag="JQH65_002769"
     CDS             7143..8504
                     /gene="gabP"
                     /locus_tag="JQH65_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005114089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HAY6140134.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIARATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYVPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTDLLAIGIICTVPIMAA
                     GIS"
     gene            8563..9225
                     /gene="csiR"
                     /locus_tag="JQH65_002770"
     CDS             8563..9225
                     /gene="csiR"
                     /locus_tag="JQH65_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HAY6140135.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGSDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(9226..9675)
                     /gene="kbp"
                     /locus_tag="JQH65_002771"
     CDS             complement(9226..9675)
                     /gene="kbp"
                     /locus_tag="JQH65_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HAY6140136.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(9759..9917)
                     /locus_tag="JQH65_002772"
     CDS             complement(9759..9917)
                     /locus_tag="JQH65_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HAY6140137.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            10100..10399
                     /locus_tag="JQH65_002773"
     CDS             10100..10399
                     /locus_tag="JQH65_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY6140138.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            10409..10933
                     /gene="ygaP"
                     /locus_tag="JQH65_002774"
     CDS             10409..10933
                     /gene="ygaP"
                     /locus_tag="JQH65_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HAY6140139.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDRIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(10980..11384)
                     /gene="stpA"
                     /locus_tag="JQH65_002775"
     CDS             complement(10980..11384)
                     /gene="stpA"
                     /locus_tag="JQH65_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HAY6140140.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            12051..12500
                     /gene="alaE"
                     /locus_tag="JQH65_002776"
     CDS             12051..12500
                     /gene="alaE"
                     /locus_tag="JQH65_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HAY6140141.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(12537..12881)
                     /locus_tag="JQH65_002777"
     CDS             complement(12537..12881)
                     /locus_tag="JQH65_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HAY6140142.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
BASE COUNT         3217 a         3165 c         3295 g         3231 t
ORIGIN      
        1 atcagggaaa atatgtctga gtttgcacgt tgccattatg ggtatataca aatccctcca
       61 gtgacaactt tccgcgccga cggaccagaa actcccgaag aagaaaaagg ttactggttt
      121 catgcttatc aacccgaaga tctttgtacc atccataacc caatgggaga tttgcaggat
      181 tttatcgcat tggttaaaga tgctaaaaaa tttggtatcg atatcattcc tgattataca
      241 tttaacttta tgggaatcgg gggtagtggt aaaaatgacc tggattatcc ctctgctgat
      301 atacgagcga agatcagtaa agatatagaa agcggtatcc ctggctattg gcaaggtcag
      361 gttttgattc cattcattaa agatccagta acaaaagaac gtaaacaaat ccatccagaa
      421 gatatacatc tcactgcaaa agacttcgaa gcaagtaaag ataacatctc taaggatgaa
      481 tgggaaaacc tccatgcatt aaaagaaaag cgtttaaatg gaatgcctaa aacaacaccc
      541 aaaagtggcc aggttattat gttgcaaaat caatacgttc gagaaatgcg aaaatatggc
      601 gtacgaggtt tacgttatga tgcggcaaaa cactcaaaac atgaacaaat agagaggtcg
      661 ataaccccac cgcttaaaaa ttataatgag cggttacaca atactaattt atttaaccca
      721 aaatatcaaa aaaaagccgt tatgaattac atggaatacc tggtaacttg tcagttggat
      781 gaacaacaaa tgtcatcact gctttatgag agagatgatt taagtgccat tgatttttct
      841 ttactcatga aaacgataaa agccttttca tttggtggag atctccaaac ccttgcatca
      901 aaaccgggtt ccacaatctc aagtatccca tcagaaagac ggatattgat taacattaac
      961 cacgattttc ctaacaatgg taatcttttc aatgactttc tatttaacca tcaacaagat
     1021 gaacaattag caatggcata tatggccgct ctcccgttca gcagaccttt agtttactgg
     1081 gatggccaag tattaaaatc aacgactgaa attaaaaatt atgatgggtc cacgcgtgtc
     1141 ggcggtgagg cgtggcttaa taaaggttgc tctacctatc agcagctcta caatgaattc
     1201 cacgcattat atatagataa agcaggaata tggagcgcat ttgagggtgt atttgcaact
     1261 aaaaacgttc tggcctttag tcgtggggat tctgtgaaca ttaatcactc tcctcatgat
     1321 gggctagtta taataaataa aggaaacgaa gaagttgaag gtacctggcc taacaaattg
     1381 caacctggaa tatacaaaaa catggggagt aatagcgtta acattattat taataatacc
     1441 cgaaaaatta tcccccctgg taaagtattt acgcttagag gcggaactct aaatatcaat
     1501 attcctgggc gtagcgctct tcttttaggg aaaacaggag aaccgccgaa ctatctctat
     1561 ttataattta atgttatatc tgccccgata aaacggggca gataatatgt ttagtttact
     1621 aacggtcatt ttgcagtgaa gccatttact gttttttatc gaccagataa tctgttctct
     1681 aatgttaact ccccctaacc tgttgcttta gttattcatt tcctgtctca ctttgcctta
     1741 ataccctacg ttaaatgtta ctaatttgtt gtttttgatc acaataagaa aacaatatgt
     1801 cgcttttgtg cgcatttttc agaaatgtag atatttttag attatggcta cgaaatgagc
     1861 atcgccatgt caccctacat ctcataagag gatcgcttct gatgaatgca ctgatcgccg
     1921 tacaaaataa cgctgtcgat tcaggccagg actatagcgg attcactctc atcccgtcgg
     1981 cgcaatcccc gcgtctgctg gaactcacct tcaccgaaca gacgaccaaa cagtttctcg
     2041 aacaggttgc cgaatggccc gtgcaggcgc tggagtacaa atcgtttctg cgttttcggg
     2101 taggcaaaat tcttgacgat ctgtgtgcga atcagctgca accattgctg ttgaagcccc
     2161 tgctaaaccg tgctgaaggt gcgctgttga tcaatgcggt gggtatcgat gatgtggcgc
     2221 aggcagatga gatggtgaag ctggcaacgg cggtagcgca tctgattggt cgctcaaatt
     2281 tcgatgcgat gagcggtcag tattacgcgc gtttcgtggt gaaaaatgtc gataactcag
     2341 acagctatct gcgtcagccg caccgcgtga tggaactgca caacgacggc acgtatgtcg
     2401 aagagatcac cgattacgtg ctgatgatga aaatcgatga gcaaaacatg cagggtggaa
     2461 attcgttgct gctgcatctc gatgactggg aacatctgga ccactatttc cgccatccac
     2521 tggcgcgtcg cccgatgcgc tttgccgcgc cgccgagcaa aaacgtcagc aaagatgttt
     2581 tccatccagt gttcgacgtt gatcaacagg gtcgcccggt gatgcgctat atcgaccagt
     2641 tcgtccagcc aaaagatttt gaagaaggcg tgtggttgag cgagctttcc gatgccattg
     2701 aaaccagcaa aggcattctt tctgtacccg ttcccgttgg caaattcctg ttgataaaca
     2761 acctgttctg gctgcacggt cgcgaccgct ttactccgca cccggatctg cgccgtgaac
     2821 tgatgcgtca gcgtggctat ttcgcttacg ccactcacca ctaccagacg catcagtaag
     2881 cgcaaaggaa ttgagcggat gtatgatttt gtgattattg gcggcggcat catcggcatg
     2941 tcgaccgcca tgcaactgat tgatgtttat ccggatgccc gcattgcgtt gctggaaaaa
     3001 gagtccggcc cggcctgtca ccagacgggc cacaacagcg gcgtgatcca tgccggggtc
     3061 tattacacgc ccggcagcct gaaggcgcag ttttgcctgg cgggaaaccg cgccaccaaa
     3121 gccttttgcg atcaaaacga cattcgctat gacaactgcg gcaagatgct gatcgccacg
     3181 tccgatctcg aaatggaacg gatgcgcgcg ttgtgggaac gcacggcggc gaacggtatc
     3241 gagcgcgagt ggttaaacgc cgaggaactg cgcgagcgcg aaccgaatat caccgggctc
     3301 ggcggcattt ttgtgccgtc cagcggcatt gtcagctatc gcgaagtcac agcggcaatg
     3361 gcaaaaatct tccaggccag aggcggtgag attatttata acgccgaagt cagcgccctc
     3421 agtgagcata aaaacggcgt ggtcgtccga acccgtcagg gcggcgaata tgaagcatcg
     3481 acgctgatta gctgttccgg gctgatggct aaccggctgg tgaaaatgct cggcctcgaa
     3541 ccgggtttta ttatctgccc gttccgtggc gagtatttcc gccttgcgcc ggagcataac
     3601 cagattgtta accatctgat ttaccccatc cctgacccgg caatgccgtt tttgggcgtg
     3661 catctcaccc gcatgatcga cggcagcgtg accgtcgggc caaacgcggt gctggctttt
     3721 aaacgcgaag gctatcgcaa gcgcgacttc tcatttagcg acacgctgga aattttaggc
     3781 tcgtcgggga ttcgccgggt gctgcaaaac catctacgct caggactggg cgagatgaaa
     3841 aactcgctgt gcaaaagcgg ctatctgcgg ctggtgcaaa agtattgtcc ccggctttcg
     3901 ttaagcgatc tccagccctg gcccgccggt gtgcgggcgc aggcggtatc gccggacggc
     3961 aagctgattg acgattttct gtttgtcacc accccgcgca cgatccacac ctgcaatgcg
     4021 ccctccccgg cagcgacatc agcaattcct attggtgcgc atattgtcag caaggtacaa
     4081 acgctgttgg caagccagag taaccccgga cgcacgctgc gagcgacacg tagtgtggat
     4141 gccttacacg ccgcatttaa tcaataacct ttgaaaacag gacgtaacaa tgaaacttaa
     4201 cgacagtaac ttattccgcc agcaggcgtt gattaacggg gaatggctgg acgccaacaa
     4261 tggcgggatc atcgacgtca ccaatccggc gaacggcgac aagctgggta gcgtgccgaa
     4321 aatgggcgct gatgaaaccc gcgccgctat cgacgccgcc aaccgcgccc tgcccgccag
     4381 gcgtgcgctc accgccaaag aacgcgccaa cattctgcgc aactggttca atttgatgat
     4441 ggagcatcag gacgatttag cgcgcctgat gaccctcgaa cagggcaaac cactggccga
     4501 agcgaaaggc gaaatcagct acgctgcctc ttttattgag tggtttgccg aagaaggcaa
     4561 acgtatttat ggcgatacca ttcccggtca tcaggccgat aagcgcctga ttgttatcaa
     4621 gcagccgatt ggcgttaccg cagctatcac gccgtggaac ttcccggcgg cgatgattac
     4681 ccgtaaagcc ggtccggcgc tggcggcagg ctgcaccatg gtgctgaagc ccgccagtca
     4741 gacaccgttc tctgcgctgg cgctggcgga gctggcgatt cgcgcgggca ttgcggctgg
     4801 cgtgtttaac gtggtcaccg gttcggcagg cgcggtcggt aatgaactga ccagcaaccc
     4861 gctggtacgc aaactgtcgt ttaccggttc gaccgaaatt ggccgacagt taatggaaca
     4921 gtgcgcgaaa gacatcaaaa aagtgtcgct ggagctgggc ggcaacgcgc cgtttatcgt
     4981 ctttgacgat gccgatctcg ataaagccgt ggaaggcgcg ctggcctcga aattccgcaa
     5041 cgccgggcaa acctgcgtct gcgccaaccg tttggcgtgt atgaccgctt tgccgaaaaa
     5101 ttgcagcagg cggtgagcaa actgcacatc ggcgacgggc tggataaagg cgtcaccatc
     5161 gggccgctga tcgatgaaaa agcggtagca aaagtggaag agcatattgc cgatgcgctg
     5221 gagaaaggtg cgcgtgtggt ttgcggcgct aaagcggatg aacgcggcgg caacttcttc
     5281 cagccgacca ttctggtgga cgtgccggcc aacgccaaag tgtcgaaaga agagacgttc
     5341 ggccccctcg ccccgctgtt ccgctttaaa gatgaagccg atgtgattgc gcaagccaat
     5401 gacaccgagt ttggccttgc cgcctatttc tacgcccgtg atttaagccg cgtcttccgc
     5461 gtgggcgaag cgctggagta cggcatcgtc ggcatcaata ccggcattat ttccaatgaa
     5521 gtggccccgt tcggcggcat caaagcctcg ggtctgggtc gtgaaggttc gaagtatggc
     5581 atcgaagatt acttagaaat caaatatatg tgcatcggtc tttaactgga gaatgcgaat
     5641 gaacagcaat aaagagttaa tgcagcgccg cagtcaggcg attccccgtg gcgttgggca
     5701 aattcacccg attttcgctg accgcgcgga aaactgccgg gtgtgggacg ttgaaggccg
     5761 tgagtatctt gatttcgcgg gcgggattgc ggtgctcaat accgggcacc tgcatccgaa
     5821 ggtggtggcc gcggtggaag cgcagttgaa aaaactgtcg cacacctgct tccaagtgct
     5881 ggcttacgag ccgtatctgg agctgtgcga gattatgaat cagaaggtgc cgggcgattt
     5941 cgccaagaaa acgctgctgg ttacgaccgg ttccgaagcg gtggaaaacg cggtaaaaat
     6001 cgcccgcgcc gccaccaaac gtagcggcac catcgctttt agcggcgcgt atcacgggcg
     6061 cacgcattac acgctggcgc tgaccggcaa ggtgaatccg tactctgcgg gcatggggct
     6121 gatgccgggt catgtttatc gcgcgcttta tccttgcccg ctgcacggca taagcgagga
     6181 tgacgctatc gccagcatcc accggatctt caaaaatgat gccgcgccgg aagatatcgc
     6241 cgccatcgtg attgagccgg ttcagggcga aggcggtttc tacgccgcgt cgccagcctt
     6301 tatgcagcgt ttacgcgctc tgtgtgacga gcacgggatc atgctgattg ccgatgaagt
     6361 gcagagcggc gcggggcgta ccggcacgct gtttgcggtg gagcagatgg gcgtggcgcc
     6421 ggatcttacc acctttgcga aatcgatcgc tggcgggcgt caccgggcgc gcggaagtga
     6481 tggacgctgt tgcaccgggc ggtctgggcg gcacctatgc cggtaacccg attgcctgcg
     6541 tggctgcgct ggaagtgttg aaggtgttcg agcaggaaaa tctgctgcaa aaagccaacg
     6601 atctggggca gaagctaaaa gacggattgt tggcgatagc cgaaaaacac ccggagatcg
     6661 gcgacgtacg cgggctggga gcgatgattg ccatcgagct gtttgaagat ggcgatcaca
     6721 gcaaaccgga cgcaaaactc accgccggga tcgtggcacg cgcccgcgat aagggcctga
     6781 ttcttctctc ctgcggcccg tattacaacg tgctgcgcat ccttgtactg ctcaccattg
     6841 aagacgctca gatccgtcag ggtctggaga ttatcggtca gtgttttgat gaggcgaagc
     6901 agtagcaccg tccccatgca ggaggcgacg ctgcgcgtct tgtccggcct acgagaatcc
     6961 aggtcggata aggcgtttac gccgcatccg acaatctgta cgtgaacagg aataaatcta
     7021 tgtcggccgg ataatgcgga gccgctctcc ggcaaaaaga atcaataaca attaaacgcg
     7081 tgacccggcg cgggaaatgt cggggcgctc tcccaagtga cacactttcg agaggattca
     7141 ggatggggca atcatcgcaa ccacatgagt taggcggcgg gctgaaatca cgccacgtca
     7201 ccatgttgtc tattgccggt gttatcggcg caagtctgtt tgtcggttcc agcgtcgcca
     7261 tcgccgaagc gggcccggca gtattactgg cctatctgtt cgccgggcta ctggtggtta
     7321 tgattatgcg gatgttggcg gaaatggcgg ttgccacgcc cgataccggt tcgttttcca
     7381 cctatgccga taaagccatt ggtcgctggg cgggctatac catcggctgg ttgtactggt
     7441 ggttttgggt actggttatc ccgctggaag ccaacatcgc cgccatgatc ctgcactcgt
     7501 gggttccagg cattcccatc tggttatttt ccctcgtcat tacccttgcc ttaactggca
     7561 gtaatttatt aagcgttaaa aactacggcg aatttgagtt ctggctggcg ctgtgcaaag
     7621 tcatcgctat cctggccttt attttccttg gtgcagtcgc aattagcggt ttttaccctt
     7681 atgccgaagt gagcgggatc tcaagattgt gggatagcgg cggctttatg cccaacggct
     7741 tcggtgcggt attaagcgcg atgttgatca ccatgttctc gtttatgggc gcagaaattg
     7801 tcaccattgc cgccgcggaa tccgacacgc cggaaaaaca tattgcccgc gccactaact
     7861 cggttatctg gcgtatttct atcttctatt tgtgctctat ttttgtcgtc gtggcattaa
     7921 ttccgtggaa tatgcccgga ctaaaagccg tcggttctta tcgctcggta ctggaattgc
     7981 tcaatattcc ccatgcgaaa ttaatcatgg actgtgtgat attactttcc gtaaccagct
     8041 gcctgaactc ggcgctgtat accgcttcca ggatgctcta ctccttaagc cgtcgcggag
     8101 atgcgcctgc ggtaatgggt aaaatcaacc gcagtaaaac cccgtacgtg gcggtgttac
     8161 tctccaccgg cgcggcattc ttaacggtgg tggtgaacta ttacgtgcct gcgaaggtat
     8221 ttaaatttct gatcgacagc tccggcgcta tcgccctgct ggtttattta gtcatcgccg
     8281 tttcacagtt gcggatgcgc aaaattctgc gagcagaagg aagcgaaatt cgcttgcgca
     8341 tgtggcttta tccctggctc acctggctgg tcatcggctt tattaccttt gtgttggtag
     8401 tgatgctatt ccgcccggcg caacagttag aagtgatctc caccgactta ttagcaatag
     8461 ggattatctg taccgtgcca attatggcgg ctggaataag ctgataatgt ggcaaaaaac
     8521 acccgttcat aatacgcgct gataataatc aggagtcaca ccatgaccat tacgtctctg
     8581 gatggctatc gctggctgaa gaacgatatt attcgcggta attttcaacc ggatgaaaaa
     8641 ttacggatga gtttgctgac atcgcgttat gcacttggcg ttgggccgtt gcgggaagca
     8701 ctttctcaac tggtggcgga acggctggtc acggtggtga atcaaaaagg gtatcgggtg
     8761 gcgtctatgt cagagcagga gctgctcgat attttcgatg cccgcgccaa tatggaagcg
     8821 atgttagtga gtctggcgat tgcccgcggt agcgatgagt gggaggcaga cgttctcgca
     8881 aaagcgcatc tgctcagtaa gctggaagcc tgtgacgcca gcgagaaaat gcttgatgag
     8941 tgggatctgc gtcatcaggc gtttcataca gcaattgtgg cgggctgtgg ttctcactat
     9001 ttgctgcaaa tgcgtgaacg gttgtttgat ctggcggcgc gctatcgatt tatctggctg
     9061 cggcgaacgg tgctttcggt agaaatgctg gaggataaac acgatcagca ccagaccctg
     9121 actgcggcgg tactggcgcg agataccgcg cgcgccagtg agttaatgcg ccagcattta
     9181 ctgacgccaa ttcccattat ccagcaggcg atggctggca attaactact cttccggaat
     9241 acgcaacact tgccccggat aaattttatc cgggcttttt agcatcggtt tattcgcttc
     9301 gaagatttta ttgtacagat tagcgttacc gtagacctgt ttggaaatgg cactcagagt
     9361 gtcgccagac ttaacggtat aaaactggct ggcagtggct ggtgtcgccg ttttcacctg
     9421 atcatcaaca ctggcaatac cggaaatatt ccccaccgca acaaggattt tctccttcgc
     9481 ctcctgactc aggccgtcac cagtgaccgt cgctttgccg tcggcaattt gaatgttcac
     9541 tttatcggca tcaggtatac cggttttgct cagatgctcc tgcaccttct tcgcctgatc
     9601 gtctttatcg tgctgacctg taaccgcgtc ccagagtttt tctccggcat ctttcacaaa
     9661 attgaacaga cccatagcta cctcattcgt taacggaaac actcagaaag tgtagcagag
     9721 gaacgcggcg cgttaacgcc gcggggggga gcacaggttt aatcgcgggt ttgcacccag
     9781 aacgcgtgaa tcaaaccggg gatataccca agcagtgtca gcagaatatt aataatgaac
     9841 gcccaaccga accctttacc gagcagcacg ccgagcggcg gcagaatgat ggtgatgacg
     9901 attctccaga aacccatatg tactccctat aagaaaatta ctcattgttt aaaaagagat
     9961 tttatctctt aagcgtagta attttagcgg agcctgccag tttcagctta cgcttttact
    10021 ctcatttacg ctgctgaggc tggtatatgt tgcattttta gtttagagtt tacttaattt
    10081 agaaaatact taaataaata tgactgaact cgcgcaatta caggccagtg ccgaacaggc
    10141 agcggcctta ttgaaagcaa tgagccatcc taaacgattg ctgattctgt gcatgcttag
    10201 cggttccccc ggcaccagcg cgggagagct gacgcgcatt accggactga gtgcctctgc
    10261 gacatcacag catctcgctc gtatgcggga cgaagggctt atcgacagcc aacgggatgc
    10321 ccaacgcatt ctatattcca ttaaaaatga ggcggtaaat gccattatcg ccaccctgaa
    10381 aaatgtctat tgtccgtaag gagtcactat ggctttgaca accatttcgc cgcatgatgc
    10441 acaagaatta atcgcacgcg gcgcaaagtt aatcgatatt cgtgacgctg atgaatatct
    10501 tcgtgaacat attcctgaag cagatctggc tccattatcc gtgctggaac agtcaggtct
    10561 tccacctaaa ttacgtcgcg agcaaattat attccactgc caggcaggta aacgcaccag
    10621 taataacgcc gataaattag cagcgattgc cgcccccgca gaaatatttt tactcgaaga
    10681 taggattgat ggctggaaaa gagctggatt gccagtagcg gtaaataaat ctcaaccctt
    10741 gccgttaatg cgccaggtgc agatcgctgc gggtggttta atattaatcg gcgttgtact
    10801 gggttatacc gtaaatagcg gtttcttctt attaagtggc tttgttggtg ccgggttact
    10861 gtttgcagga atcagcggtt tttgtggaat ggcaaggttg ttagataaga tgccgtggaa
    10921 ccaacgagct tgagaagcga cgccggacgc gccctagcag cgacatccgg cctcagtaat
    10981 tagatcagga aatcgtcgag agatttacct tctgccagcg cctgagcaat tggcttcggt
    11041 gtacggccct gaccggtcca ggtcttagtt tcaccgttaa catcagtgaa tttatatttc
    11101 gccggacgcg gctggcgttt tttaccagca cgtggtgcag cagcagagct attacccaat
    11161 aactcttccg ggttaattcc gtcagctttc atcagctcca gccaggtgct aattttttcc
    11221 tgacgctcag ccagttcacg ctgctgctgt tcttcttctt cacgtctttc tttagtgaca
    11281 accctgaatt tttcgagcat ttcttcaaga acgtcaatgg agaattcgcg agccatcgca
    11341 cggagggttc gaatgttatt taaactttgt aacattacgg acataataaa gaaaaccttt
    11401 taacgccaaa acaaaaaagt atttcgtcgc gtagtgtaag cgatcactta ttatatttcc
    11461 acaaaattta atctatttac agtcctgcgc gagattattt cagctgttat tttctgaaac
    11521 tctcagaata atttcaagat tatatattag ccattttgat gagtaattta ggttattggt
    11581 gaacagagtg gcgctctctg ctcgctaact ccgcaagcct gataaaaccg aagtgaataa
    11641 aaatgcaata taacagaata aaagaaaaca cccctcactg gtgctttctg ctgttaatgt
    11701 acattttctg gcatcaaaaa tgctgtaaat gacccggtca cgacaaacac cgaattctct
    11761 acgcttatag cgttacctca cccccaaact ttcattagtg aaattaaagg caaggtcata
    11821 tccaattgat ttaattgaaa ataaatcata tcggcatgta aaaatccaca atgtacaaaa
    11881 aacgagctgt tacggagtat tttatctaca aaaactgact aaataaaagt ttttcactaa
    11941 ttgattagtc atagccagcg atatacgcta tgcgaaaatg cagatggcaa tgagatccac
    12001 tgctttcatc tccattaaca tcccattacg ctttattaag gagcattagc atgttctcac
    12061 cgcagtcacg cttgcgtcat gcagttgcag atacgttcgc gatggttgtt tactgttctg
    12121 tcgtgaacat gtgtattgaa gttttcctct ccggaatgag cttcgaacag tctttttatt
    12181 ccagattggt agcgattccg gtgaacatct taattgcatg gccatacggt atgtaccgcg
    12241 atctgtttat gcgcgcggca cgcaaagtta gcccgtcggg ctggataaaa aatctggctg
    12301 atatcctggc ttatgtgacg ttccagtcac cggtgtatgt ggcgatcttg ttagtggtgg
    12361 gcgcagactg gcatcagatt atggcggcgg tcagttcaaa catcgttgtt tcgatgttga
    12421 tgggggcggt ttatggctac ttcctcgatt attgccgccg actgtttaaa gtcagccgtt
    12481 accagcaggt aaaagcctga ctcttctttt cgcgactggc atcgccagtc gcgtctttaa
    12541 ctggcttcgc caaataaacc attcaaataa cgttcaagcg caacacgcga actgaagcca
    12601 tgcgggatac cgtaatgctc gtgtcgctcg cctgctaaat agagcggaaa ttgctggtaa
    12661 tgatcgcaag ttttaatatc ggaagcgggt tcagccagtg ttgaactgcg ttcttttaat
    12721 gttggatgaa ttaccagagc ggtacgtccc attcgcgctt cgcgattaac atagacataa
    12781 ttttccccac ggcggtaacc atacgctttc tgggttacca catcgacagt aaaaccgact
    12841 ttttcaagta cgcgcgccac ctcgtctggt cgtaaataca tatttgatcc tcgttatttt
    12901 caaccgcg
//
LOCUS       DADLFA010000117        12908 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000117 DADLFA010000000
VERSION     DADLFA010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12908
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.117"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(75..1613)
                     /locus_tag="JQH65_002778"
     CDS             complement(75..1613)
                     /locus_tag="JQH65_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005147267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HAY6140143.1"
                     /translation="MTLCGPGSVSSCGQQAQTRGLKHLFVMADSFLHQAGMTAGLTRS
                     LAVKGIAMTLWPCPVGEPCITDVCAAVAQLRESGCDGVIAFGGGSVLDAAKAVALLVT
                     NPDSTLAEMSETSVLQPRLPLIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVL
                     SDETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSA
                     RLQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFN
                     IPTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEF
                     NRIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPE
                     YLYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYI
                     MGMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDP
                     SLFF"
     gene            complement(1687..2523)
                     /gene="eutJ"
                     /locus_tag="JQH65_002779"
     CDS             complement(1687..2523)
                     /gene="eutJ"
                     /locus_tag="JQH65_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HAY6140144.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVAELLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(2534..3937)
                     /locus_tag="JQH65_002780"
     CDS             complement(2534..3937)
                     /locus_tag="JQH65_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HAY6140145.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(3949..4236)
                     /gene="eutN"
                     /locus_tag="JQH65_002781"
     CDS             complement(3949..4236)
                     /gene="eutN"
                     /locus_tag="JQH65_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HAY6140146.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(4343..4636)
                     /gene="eutM"
                     /locus_tag="JQH65_002782"
     CDS             complement(4343..4636)
                     /gene="eutM"
                     /locus_tag="JQH65_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HAY6140147.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(4675..5691)
                     /gene="pta"
                     /locus_tag="JQH65_002783"
     CDS             complement(4675..5691)
                     /gene="pta"
                     /locus_tag="JQH65_002783"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAY6140148.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSFIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YNIAQRLGGYRAVGPLIQGLAVPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(5681..6490)
                     /gene="eutT"
                     /locus_tag="JQH65_002784"
     CDS             complement(5681..6490)
                     /gene="eutT"
                     /locus_tag="JQH65_002784"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001425897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HAY6140149.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQKPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVLEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGERNDY"
     gene            complement(6487..7188)
                     /gene="eutQ"
                     /locus_tag="JQH65_002785"
     CDS             complement(6487..7188)
                     /gene="eutQ"
                     /locus_tag="JQH65_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HAY6140150.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQQPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGVLHVRHEGQTMIAKAGDVMFIPKGSSIEFGMTSS
                     VKFLYVAWPANWQSL"
     gene            complement(7163..7642)
                     /gene="eutP"
                     /locus_tag="JQH65_002786"
     CDS             complement(7163..7642)
                     /gene="eutP"
                     /locus_tag="JQH65_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HAY6140151.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(7655..7990)
                     /gene="eutS"
                     /locus_tag="JQH65_002787"
     CDS             complement(7655..7990)
                     /gene="eutS"
                     /locus_tag="JQH65_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HAY6140152.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLISHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(8282..10561)
                     /gene="maeB"
                     /locus_tag="JQH65_002788"
     CDS             complement(8282..10561)
                     /gene="maeB"
                     /locus_tag="JQH65_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HAY6140153.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGVQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            10850..11800
                     /gene="tal"
                     /locus_tag="JQH65_002789"
     CDS             10850..11800
                     /gene="tal"
                     /locus_tag="JQH65_002789"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HAY6140154.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGISAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            11820..>12908
                     /locus_tag="JQH65_002790"
     CDS             11820..>12908
                     /locus_tag="JQH65_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAY6140155.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLLNDFL
                     KHNPTDPTGYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNT"
BASE COUNT         2876 a         3697 c         3354 g         2981 t
ORIGIN      
        1 tcccattgac gccatcacgc tgcgtacaat ttcttcaatc tgtttttgat ccatgatgtg
       61 ttatctccgc gtcatcagaa gaacagtgac ggatcgcccg cccgtttggt caggcgaccg
      121 tttgccataa tgcccatgct ttccagccag cgttcaaact ctggtgacgg gcgcaggttg
      181 agtaactgac gcacagtggc ggtatcgtgg aatgcggtgg tctggtagtt gagcatgatg
      241 tcatcaccca gcggcatccc catgatgtag ttgcagcctg cggtggcgag caggatcatc
      301 aggttttcgt tgaggttctg gtcagcgtca gcgtggttgg tgtagcagca gtcacagccc
      361 atagagatgc cgctcagctt gcccataaag tgatcttcta agccagcacg gataatctgg
      421 cggtcgttgt agagatactc cggcccaata aagccgacca cggtgttgac gataaacgga
      481 tcgtaatgac gcgccagccc atagttacgt gcttccatcg tcacctggtc agcgccgaag
      541 ttagcgccag cggatagcgc agagccttgt ccggtttcga agtagaggca gttttccccg
      601 gcgatacggt tgaactctgc acccactgcg cgcgcttcgt cgagcatcgc cagttccacg
      661 ccaaactctt tcagcccttt ttcgctgcca cagatactct ggaaaatcag tccgccaggc
      721 gcgccgcgac gaatcgcttc gatctgggtg gtgacgtgcg ccagtacgca gccctgagtt
      781 gggatgttga atttgtcgat cacgccataa atggtatcca gcacgcggct taagttttcc
      841 acgtcgtcag ttaccgggtt gacgccgatc accgcatcgc ccaccccgaa ggaaagccct
      901 tcgtagattt gcgcggcgat actttgcacg tcgtcacgag tgtcatttgg ctgcaaacgg
      961 gcgctgaagg tgcccggaat accgatggtg gtattggcct ttttgattac cggcattttc
     1021 ttcgcgccgt agatcaggtc cgcgttggag caaatcttcg ctaccgccgc gaccacttcc
     1081 gaggtcagcc ctttgcgggt aaaggcaatg tcgtccacgc tagtttcatc gctcagcaca
     1141 tactcacgca gttcgctgat gctccagttt ttaatctggt tgtaggccgt ttcgttaaca
     1201 tcgtcctgaa tcagccgcgt cacgcagtca tcttcatagg caatcagcgg caagcgcggt
     1261 tgcagaacgc tggtttctga catctctgcc agcgtgctat ccgggttcgt caccagcaac
     1321 gccacggctt tcgccgcatc cagcaccgag ccgccgccaa atgcgatcac cccatcacag
     1381 cctgactcac gcaactgcgc cacggctgca cacacgtcgg tgatgcacgg ttcgcccacc
     1441 ggacatggcc agagcgtcat ggcgatgcct ttaacagcca ggctgcgcgt cagcccggcg
     1501 gtcatccctg cctgatgcaa aaagctgtct gccatcacga acagatgttt cagcccacgc
     1561 gtttgcgctt gctgtccgca actgctcacc gagcccggac cgcaaagcgt caccggtgga
     1621 acgctaaatg tttttacccg ttgcagattc agggtatcga acgcctgaaa gagcgcggtc
     1681 tgcaattcat tttgcatata gcccctccgc tttctctcgc ccactactgg cgatcgccag
     1741 cggggtcata aacaaactgt gctgcggtaa atgcacctgt aacgccggga attgtttgcg
     1801 aaacagttcc gccacgcccg gttgcataca agagccgccc gccagccata aatcagtaat
     1861 gccctgccct tcaatatgcc gggcgacaat gtccgccatt ttttcgtaca ccggtttcac
     1921 cgtaggccaa atctcttcac cgtgaccgcg cttgtactgc tccgcctctt ccagcgaaat
     1981 acggcgattt ccggcgagag tcagagagat gtgatgtccg ccggttgctt catccgccga
     2041 gtacgtcacc ttgccctttt tcacgatggc aatgccggta gtgccgccgc cgatatccac
     2101 tacaccggcg ttgtccagtt gcagcagctc cgcaactgcg gttggctcat ccagcacatg
     2161 gctcacttcc agtccagcag actccagcac gttgatggaa atacgcgggt ccgtacctgg
     2221 aggaaatgaa gtcgccgcat ggctaaaacg gcgaccgaat tgctgctcga gcgtgtcgag
     2281 atggcgacga acaatggtga cagcgccgaa gaaatcccag acgatgccgt cgcggaccac
     2341 atcggcccag tcgaggcata ccgccaccgg ctgaccgtcg cggtcgacaa ccatcgacac
     2401 cacatcgcag gtgcccagat ccacccccag ccacagcgga gattccgtcg cggcgggcgt
     2461 ctggttacac agcgttgccg ccgtttgcag acgtggggtg agccattgtt cgtcgtgcgc
     2521 catctgttac tccttaaaca atgcgaaacg catcgactaa tacacagcga cgcagacgga
     2581 caaacgtacg cgcgctggtt accccttcac cggttggcgt ggtgatggtc atggtggtcc
     2641 agccttcccc gcccagcccc agcccggcaa tgcacggtcc gttcttaacg aaaatgctgg
     2701 tatcaatagc attcgccatc tggttcatgt tttcgatgtt gcgcgagtgc attgccgccg
     2761 tgtggtggca accgccttcc agtttcaccg ccagcgcaat ggcatccgcc acgttggcga
     2821 cgcgcacgac gggcagcacc ggcatcatca gttcggtcac ggcaaacgga tgttctgcag
     2881 tggtttccac aaacagcaaa cgcgtttctt gcggcacgtt caggccgatt gccgccgcga
     2941 ttttcgccgc atcgcgacca acccagtcac ggctgacggt gccttttccg cgctcgtcga
     3001 tatttttcag caacaccggt tgcagctgct gcgcctgttc tgcggtcagt ttcaccgcgt
     3061 gctggccttc catcagacgc atcagttcat cggctacgct atcaacaaca atcagtacct
     3121 tttcgtcggc acaaatgatg ttgttatcga aagaagcgcc tttgacgatg gactgagcgg
     3181 cacgggcgag gtcggcggtt tcatccacca ctaccggcgg gttgccagcg cctgcggcaa
     3241 tcagacgttt attggtgtgt ttacgcgccg cttctactac cgcttcgccg ccggtcacca
     3301 ccagaaggcc gatgcccgga aacttgaaca aacgctgcgc ggtttcgata tccggatttg
     3361 ccacagtaac cagtaagttt tccggcccgc ctgcggcaac aatcgcctgg ttgagcagcg
     3421 tgatcgcccg ctgggagact tttttcgccg ccggatgcgg ggcaaaaatg acgctgttgc
     3481 ccgcggcaat caggctgatg gcgttattaa ttacggttgc cgccgggtta gtggacggag
     3541 ttaccgaagc caccacgccc cagggcgcgt tttcaattaa agtcaggccg ttgtcgccgg
     3601 tcagcacctg cggagagaga cactcaacgc ctggcgtgcc gcgcgcctga gcgacgtttt
     3661 tggcaaattt atcttcaacg cggcccatgc cggtttcact gacggcaagt tccgctaaat
     3721 ctctggcgtg tttttcgcct gcctcacgaa tggcagcaat ggctaactgg cgcattgcca
     3781 cgctttttaa cccttgctgg gcgactttgg ctgccgcaac ggcgtcatcc agggacgcga
     3841 aaacgcccat ctcatgaacg gcggcgggcg gcgtgtcact gctttgcatt ttcagcagta
     3901 ccgctttcac cacctgttca atatcctgtt gattcatgat gttctgcctt atttgtggaa
     3961 aattacctga ccgccagaca ccacctcatc gacaatgcca atcacgcaca gatcgaccgg
     4021 tgacgtttcg cttttatgcg cctggcgggc ggaactgcca ctcaccagta acacccactc
     4081 cccggttccc gcgccaatat tgtcgatggc gacggcgcat tgcccgtcag gattaccttg
     4141 tggatcaatc atttccacca tcagcaattt gtcatgcgcc agtccgtgat ggcgtacggt
     4201 acaaacaatt tgtccagtga cgactgccag tttcataccc gcctccgtgg cgtatttcag
     4261 gtaaaagctc cccctaccct ccgcagaagg taaaatgaaa aaggagagag cgtaacgccc
     4321 gaatcgacgt cacacagggt gattacaggt tgctgctatc gcctttcagg ccgatcggga
     4381 agacttcttc caggtcgccg tgcggacgcg ggataacgtg tacagatacc agctcgccga
     4441 tacgctgtgc cgctgcggca cctgcatcgg tcgcggcttt acaggctgca acatcaccac
     4501 gaaccatggc agtacacagg ccgccgccaa tctgcttaac accaaccagc ttgacgcgcg
     4561 cagctttcac catcgcgtca gaagcctcaa tcagtgcaac caggccccgg gtttcgatca
     4621 ttcctaatgc ttccattgtg ctttcctctt tatcagggtc cagaacggga ccgttcattc
     4681 aaccagtgtt tgtaaactgc tttcgcggtt cacttctgtc tgacgcggca cagctgccac
     4741 cagcgccagc tcgataattt cctgcacgct acaaccacga gagagatcgt gcatcggcac
     4801 ggcaagtcct tgtatcagtg gcccgacggc acgatatccg ccgagtcgtt gtgcgatgtt
     4861 gtaaccaata tttccggctt ccagcgacgg aaaaaccatc acattggcct tgccctgtag
     4921 cgggctggca ggcgcttttt gcgccgccac ttccggcacg aaggcggcgt caaactgtaa
     4981 ctcgccatcc accaccagct ttggtgcgcg ctcacggacg atttctgtcg cctgctggac
     5041 gttagcaaca caggggtgac gggcgctacc attgctggaa aacgacagca tcgccacgcg
     5101 cggctcttct ccggtgatgg cgcgccaggt ttcggcactg gcaagcgcga tatccgccag
     5161 ctgcgccgcc gtcggctgtg gcaccacgct gcaatcggca aagcccaacg ccgggccgct
     5221 gtactgtggc aacatcagga aaatgaaaga gagcgtttta cagcctggct gcaagccgat
     5281 aatgcgtaat ccggcacgca gcacatttgc cgtggaagag aggttgcctg cgatacagac
     5341 atccgcttta ccggcgctga ccattgctgc ggcgaacatc agagggtcgg taagtttttc
     5401 cagcgcatcc ggcggcgttt tttcgcccgc gcgggccagc cagcgatgag caaattcttc
     5461 ccgcattgcg aggttgccat gcggatcaat cacctgtagc ccgtccatcg ccacgccgtg
     5521 actgagcgca aactgacgaa gttcaaacgg attggcgacc agaatgggcg ttgccagacc
     5581 ttgttgatgt aaatattgcg cggctttcag cacacgttga tctaacgcat ccggaaaaac
     5641 cactctggcg ggcgctcgca acgccagttc acgacaacgt tcaataatca ttgcgttccc
     5701 ccagtcgttg ttggatctgc ttcaccgtta gcggctgctt cgttacgctc aggatcatca
     5761 tcacgtagac cgtgctggaa aggcggttca gcgcctgcaa aatgtccggg cgtaatactt
     5821 caaagctgcg ggtgataaag acctgcgccg ctacggtttc ggtttcccgc actttggtgc
     5881 gtagcagatt gagcaacgca gcatcgcgcc cgtggctggc ttccagcacc agatgatcgt
     5941 ggtcgagata gcgcagcggc tgatgagaaa gccgatgcag atcttcgtca gaaagcccga
     6001 caatcgcctg gcagcccagc ggttccccca gcgcatcggc gcgcataatg ttgccaagac
     6061 gcgaacggat atccgccagc cacggctgcc acggttccgc cagttcaatt tgcagccaca
     6121 ctgccagcgc aatggtgctg tcgagaaccg cgcgaaaacc cagacgcgga tcgcttttgg
     6181 cgaccatttt ttccgccgac aggtgagtca gcgtatccgg cttttttgcc accggctggc
     6241 ggcacagttc gcagcacgcc tgcggatgtt catcgctact ggtcagccca tgaacgggct
     6301 gcggcttctg ctgttcatcg tcaacaaaca ggcggccctg ctcgtcaata aacttgatgc
     6361 gcagatggcg gctttccagc aactcccggg cagagggcgt caggcgactg tccgcaggga
     6421 gatggatctc tgcgccttcg ctgagcgtat ggttcgctct tagccatgct tcggtgatga
     6481 aatctttcat agggattgcc agttagccgg ccaggcgaca tacaggaatt tcacgctgga
     6541 tgtcatacca aattcgatgc tggagccttt cgggataaac atcacgtcgc ctgctttggc
     6601 gatcatggtt tggccttcgt ggcgaacatg cagcacccct tccagcacca tgtcgatttc
     6661 gtcgtagttc agggtccacg ggaagaatgc gttctcccac tgcataaaac cggcggccat
     6721 gctgctgccg tcgtctccgg tcacaagatc agtcaagccc acgcaatgcg gctctgcgcc
     6781 gtcgaaacga ccaaacttga cgctgctgcc atcaatcact ttgatgccgc ctttgccggt
     6841 caccgatttg aagctcggct gcattgcgcc ctgctccagc gactgttttt ccttcatcac
     6901 tttttccatc agctgtgcga ccaggctttc ggtaaactgg ccttccggct gctgggcaat
     6961 gatggtttcg cggatgcgct ggctttcggt tttgtcggct ggcacgctgg cgggcacgga
     7021 cgccgttacc gggatcgatt catcacattc ggtgatggta aaacccagta gatccgccac
     7081 ttcacgggct tccggggtaa taatgctggc gcgcagaacc acggacattg cctgttcgcc
     7141 gcgcgcgtgt gcttcacgaa tatcattcgc tgtgatgagt tttttcacct gcttcctcct
     7201 gtttggtgag agatgccaga taatccacca gttgctgcac gctttgcggg tcgtggctgt
     7261 ttagctcgaa aatcggctct tcgaaacccg tctcaagcaa taactttcgc gttgcggcga
     7321 cgtcagcatc tggcatgtcc gttttactga tgacggcaat ttgccgtttg ctgacgccaa
     7381 tatccagcaa cccggcaggc aaccgacttt ccgggtcatt tgcgccatgg acatagatca
     7441 gcatgtctac atcctgtagc gtggtaatta aggcgtgata ccagcgggga tggctaaaat
     7501 attcacccgg cgtatcaata tcgcccttat cattaaattc cacggcctgt gtttttctgg
     7561 cgagggtata attcccctgc aacgcattaa ataacgttgt ttttcctgcc ccgacactac
     7621 ccacaaacgc aattcgtttc atgaatgcct cgtattaact tttggtcatt tcacaaagcg
     7681 tataatttaa taagcgacct aaaccgctga cggtttgtga taacgcttct tctactgcgc
     7741 ccaccgagcc ataaatcacc aatgcaccgc tgaagcggtc gagaaaaccg atatgcacat
     7801 cagcggcttt cagcgctaaa tcaccggcga tcatcgccgt ttcgccggga gttagcgtca
     7861 taatgccaat tgcgcccgca tccggaacgc cgattttctt cgccagttcc tcaccgggat
     7921 gcgagatgag atgcgccagt gtgacctgtt tgccgggcac aaattcctga atgatgcgtt
     7981 ctttatccat gagtcgccgc ctgtgaaaaa ctttcgtgta tcgtgacaat ttttcgtcag
     8041 agtaaataac aaaattcggc aagcgcgttt aaaaggtgag gtagatcact aagaatggag
     8101 agatattcgc tgtggtgcat aaactttcat atgcaacgca tgaatataaa taaaataata
     8161 tcgagatatt atatagactg cttttttatt attcgcaaac tcagtaataa gctttactaa
     8221 tatattgcag ccaaaccgct taccctgaat attcagggta agcgtgagag ttaaaaaaga
     8281 attacagcgg ttgggtttgc gcttcaacca cggccagcgc caccatgttg acgatacgac
     8341 gcaccgatgc gatcggcgtt aacacgtgaa ccggtttcgc cacgcccatc agcaccggac
     8401 cgacagtcac accttccgag ctggaaacgc gcagtaagtt gtaactaatg cgggcagctt
     8461 ccatgttcgg catcaccaga atattggcag aacctttcaa agggctgtcc ggcatacggt
     8521 cgttgcgaat cgcttccacc agcgctgcat cgccgtgcat ttcaccatca atcatcagat
     8581 ctggcgcacg atccctgacc agttccagcg cctgacgcat tttgctcgac gacgggcagt
     8641 cagaagaacc aaagttggag tgcgacaaca gagcaacgcg cggctcaata ccaaaacgac
     8701 ggacagtttc tgccgccatc aaggtgatct ccgccagctc ttctgcgtct ggttcgtcat
     8761 tgacgtaggt atcggcaata aaggtgttac cactcggcag cagcagcgcg ttcattgcac
     8821 ctgcggtgtg aacgccatcg cgataaccaa agacattttt caccacgcta aaatgttcat
     8881 gataatcgcc caccgtaccg cagatcattg catcggcttc cccacgctga accatgatcg
     8941 cgccgatcac tgtcgggtta ctgatcagcg cccgctgcgc ctgttcctga gtgacaccgc
     9001 gacgcttcat gatctggaag tattcggtcc agtactcttt aaagcgcgga tcggattcgt
     9061 tattgacgat ctcaaaatca acgcccgctt tgatctgcac gcccagtttc tgaatgcgca
     9121 tttcgatcac gttcggacga ccgataagga tcggtttcgc cagtcccagc gttaccagtt
     9181 cctgagtggc atgcagaacg cgcgcctctt ccccttctgg cagaacaacg cgcttcggcg
     9241 ctttgcgagc ctgggagaaa atcggcttca taaacaggtt ggttttgtaa acgaactcag
     9301 tcagcttgtc gatgtagacg tcgaaatcag caatcggacg cgtcgccacg cccgactcca
     9361 tcgcggcttt agcgaccgca ggagcgatct taacgatcaa gcgcggatca aacggttttg
     9421 gaatgatgta ttccggacca aagctcagat cctgatcgcc atacgctgaa gccaccactt
     9481 cgctctgttc cgcatgggcg agttctgcaa tcgcacgtac cgccgccagt ttcatctctt
     9541 cgttgatggc ggttgcgcca acgtccagcg cgccacggaa gatgaacggg aagcacagga
     9601 cgttgttcac ctggttcgga tagtcagaac gaccggtgca aatgatggca tccggacgca
     9661 cttctttcgc cagcggcggc agaatttccg gttccgggtt cgccagcgcc aggatcattg
     9721 gcgcacgagc cattttcttc accatttcct gggtcagcac tttcgggccg gaacatccga
     9781 ggaaaatatc cgcgccttca atcacatcat cgagggtacg tttgccgtcg tccaccaccg
     9841 catatgcggc tttggtttcc gccatgtttg gctcacggcc ctgatagata acgccttttg
     9901 aatcgcaaac cacgatgtta tgtttttgca gacccagcgc taccagcagg ttcatacagg
     9961 cgattgctgc ggcacccgcg ccggaaacca ccatccgcac gtcggagatg tttttctcca
    10021 ccacgcgcaa gccgttgagg atggcggcag tgctgatgat tgccgtgccg tgctgatcgt
    10081 cgtggaatac cggaatattc atccgctcgc gcagtttctg ttcaatatag aaacattctg
    10141 gcgctttaat gtcttcgagg ttgattccgc cgaaggtcgg ttcgagcgca gcgacaactt
    10201 caataaattt gtccgggtcg agttcatcaa cttcaatgtc aaatacatca atcccggcga
    10261 atttcttaaa cagaacgccc ttgccttcca tcaccggttt gccagccagc gcgccaatgt
    10321 tgcctaaccc cagcaccgcc gtaccgttag agatcaccgc caccaggtta ccgcgcgcgg
    10381 tatatttgta ggcttttaac gggtcttttt cgatttcaag acaaggtgcg gcaacgcctg
    10441 gtgagtaggc cagcgccaga tcgcgctgcg ttgccagagg cttggttgga gaaacctgga
    10501 ttttccctgg aactggaaat tcatggaaat caagtgcact ttgttttaac tggtcatcca
    10561 tttggttgtt cctttcacgt aacgttcaca aataaagtgt gtgggcaaca gcccctgccc
    10621 acaacgtggc gcacattatt accccgccgg agtctacaga ctttgagcaa gtccaaactc
    10681 tcaccattaa tataatgttt tggtaataat cctataacac tgatgttacc tgcttaatcc
    10741 agcaatacca tgcctgtctg ctatgctttt ttgatgcgtt tagcgaaatt tctcagaagt
    10801 gtgaattaac gcactcatct aacactttac ttttcaagga gtatttccta tgaacgagtt
    10861 agacggcatc aaacagttca ccactgtcgt ggcagacagc ggcgatattg agtccattcg
    10921 ccattatcat ccccaggatg ccaccaccaa tccttcgctg ttgctcaagg ctgccggatt
    10981 atcacaatat gagcatttaa tagacgatgc tatcgcctgg ggtaaaaaaa atggcaagac
    11041 ccaggaacaa caggtggtcg cagcgtgtga caaactggcg gtcaatttcg gtgctgaaat
    11101 tctcaaaatc gtacccggtc gcgtgtcaac agaagttgat gcacgcctct cttttgataa
    11161 agaaaagagt attgagaagg cgcgccatct ggtggacttg tatcagcaac aaggcgttga
    11221 gaaatcacgc attctgatca aactggcttc gacctgggaa ggaattagcg cggcagaaga
    11281 gctggaaaaa gaaggtatta actgcaacct gacgctgctg ttttcttttg cccaggcgcg
    11341 ggcctgtgcg gaagcaggcg tttttctgat ttcgccgttt gtcgggcgta tttatgactg
    11401 gtatcaggca cgcaagccga tggacccgta tgtggtggaa gaagatccgg gcgttaaatc
    11461 ggtgcgcaat atctacgact actataagca acaccactat gaaaccattg tgatgggcgc
    11521 gagcttccgt cgcaccgaac aaatcctcgc cttaaccggc tgcgatcgac tgactatcgc
    11581 accgaatttg ctgaaggagc tgcaggaaaa agtttcgcca gtggtacgta aattaatccc
    11641 accttctcag acgttcccac gcccagctcc catgagcgaa gcggagttcc gttgggagca
    11701 caatcaggat gcgatggcgg tagaaaaact gtctgaaggc attcgtctgt tcgccgttga
    11761 tcaacgcaaa ctggaagatc ttcttgccgc caaactataa accagccacg gagtgttata
    11821 tgtcccgaaa agaccttgcc aatgcgattc gcgcactcag tatggatgcg gtacaaaaag
    11881 ccaactctgg tcatcccggc gcgccgatgg gcatggctga tattgccgaa gtgctgttga
    11941 acgattttct taaacataac cctaccgacc caaccgggta tgatcgcgac cgctttattc
    12001 tttccaacgg tcacgcgtcg atgctgctct acagtttgct gcatctgacc ggttacgacc
    12061 tgccgctgga agagctgaag aacttccgtc agttgcattc gaaaacccca ggccacccgg
    12121 agattggtta tacgcccggt gttgaaacca ccaccggtcc acttggacaa ggtttggcga
    12181 acgccgtcgg actggcgata gcagagcgta cactggcggc gcagtttaat cagccggatc
    12241 atgaaattgt cgatcacttc acctatgtgt ttatgggcga cggctgcctg atggaaggta
    12301 tttcccacga agtctgttcg ctggcgggca cgctaggact gggcaagctg attggttttt
    12361 acgatcacaa cggtatttcg attgatggag aaaccgaagg ctggtttacc gacgatacgg
    12421 caaaacgttt tgaagcctat cactggcatg tgatccatga aatcgacggt cacgatccgc
    12481 aggcggtgaa ggaagcgatc cttgaagcgc aaagcgtgaa agataagccg tcgctgatta
    12541 tctgccgtac ggtgattggc tttggttcgc cgaataaagc aggtaaggaa gaggcgcacg
    12601 gcgcaccact gggggaagaa gaagtggcgc tggcacggca aaaactgggc tggcaccatc
    12661 cgccatttga gatccctaaa gagatttatc acgcctggga tgcccgtgaa aaaggcgaaa
    12721 aagcgcagca gagctggaat gagaagtttg ccgcctataa aaaggctcat ccgcaactgg
    12781 cagaagagtt tacccgacgg atgagcggtg gtttaccgaa ggactgggag aaaacgactc
    12841 agaaatatat caatgagtta caggcaaatc cggcgaaaat cgctacccgt aaggcttcgc
    12901 aaaatacg
//
LOCUS       DADLFA010000118        12773 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000118 DADLFA010000000
VERSION     DADLFA010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12773
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.118"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            4..1632
                     /locus_tag="JQH65_002791"
     CDS             4..1632
                     /locus_tag="JQH65_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001381416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAY6140156.1"
                     /translation="MFTAIIAVCILFIIGIIFARLYRRASAEQAFVRTGLGGQKVVMS
                     GGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFFVRVKPSVEGIA
                     TAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENFVQGVQNTVAED
                     LSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLIQETERRRRERNEVEQDVEV
                     AVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEAERRREAEQTRI
                     LAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQS
                     QAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETKAVELTVRAKAE
                     KEAAEMQAAAIVKLAEATRKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPA
                     VIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQALSAALSYRTQAP
                     LIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            1871..1930
                     /locus_tag="JQH65_002792"
     CDS             1871..1930
                     /locus_tag="JQH65_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HAY6140157.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            2247..2306
                     /gene="ibsE"
                     /locus_tag="JQH65_002793"
     CDS             2247..2306
                     /gene="ibsE"
                     /locus_tag="JQH65_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HAY6140158.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(2426..3859)
                     /gene="hldE"
                     /locus_tag="JQH65_002794"
     CDS             complement(2426..3859)
                     /gene="hldE"
                     /locus_tag="JQH65_002794"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HAY6140159.1"
                     /translation="MKVTLSEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(3907..6747)
                     /gene="glnE"
                     /locus_tag="JQH65_002795"
     CDS             complement(3907..6747)
                     /gene="glnE"
                     /locus_tag="JQH65_002795"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HAY6140160.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEKSLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQSPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFN
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMMLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(6770..8071)
                     /locus_tag="JQH65_002796"
     CDS             complement(6770..8071)
                     /locus_tag="JQH65_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HAY6140161.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDDSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     AYVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTKTAMAKLALTEWLVSKAWQPFLDAKAQSKM
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            8313..8933
                     /locus_tag="JQH65_002797"
     CDS             8313..8933
                     /locus_tag="JQH65_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HAY6140162.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPTRKRKERWMN"
     gene            8997..10235
                     /gene="cca"
                     /locus_tag="JQH65_002798"
     CDS             8997..10235
                     /gene="cca"
                     /locus_tag="JQH65_002798"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HAY6140163.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(10392..11213)
                     /gene="bacA"
                     /locus_tag="JQH65_002799"
     CDS             complement(10392..11213)
                     /gene="bacA"
                     /locus_tag="JQH65_002799"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAY6140164.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLPHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(11304..11672)
                     /gene="folB"
                     /locus_tag="JQH65_002800"
     CDS             complement(11304..11672)
                     /gene="folB"
                     /locus_tag="JQH65_002800"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HAY6140165.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            11777..12394
                     /gene="plsY"
                     /locus_tag="JQH65_002801"
     CDS             11777..12394
                     /gene="plsY"
                     /locus_tag="JQH65_002801"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAY6140166.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(12407..>12773)
                     /gene="ttdR"
                     /locus_tag="JQH65_002802"
                     /gene_synonym="dan"
     CDS             complement(12407..>12773)
                     /gene="ttdR"
                     /locus_tag="JQH65_002802"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
                     /protein_id="HAY6140167.1"
                     /translation="RHDCLVTKERDMTHGIWELGNGQEKKSVKVSGHLSSNSGEIVLQ
                     WALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYREPLYRSMKLRVCVEF
                     LAAWCQQRLGKPDEGYQVM"
BASE COUNT         3070 a         3610 c         3223 g         2870 t
ORIGIN      
        1 tggatgttta ccgcgattat tgccgtatgc attctgttta ttattggaat tattttcgcc
       61 aggctctatc gtcgcgcttc ggcagagcaa gcttttgttc gtactggttt aggtgggcaa
      121 aaagtggtga tgagcggtgg cgcaatcgtg atgccgatct ttcatgaaat aatccccatc
      181 aatatgaata ctctgaagct ggaagtcagc cgctcaacca ttgatagcct gattacgaaa
      241 gatcgtatgc gtgtcgatgt agtagtcgct ttctttgtac gggtaaaacc gtctgtagaa
      301 ggcatcgcca ctgcggcgca gacgttgggg caacgcacgc tatcaccaga agacttacgt
      361 atgttggttg aagataaatt tgtcgatgcc ctccgtgcaa cagctgcaca aatgaccatg
      421 catgagttac aggatacccg cgagaacttc gtgcagggag tacaaaatac tgtcgcagaa
      481 gacctgtcga aaaacggtct tgagctggaa agcgtttcac ttaccaactt taaccagacc
      541 tcgaaagaac atttcaatcc gaacaatgcc tttgacgccg aaggtttaac caaactgatt
      601 caggagacgg agcgccgtcg tcgcgaacgt aacgaagttg aacaggatgt agaagttgcg
      661 gtgcgtgaga aaaaccgtga tgcactttcg cgcaagttgg agattgaaca acaagaagcg
      721 tttatgacgc ttgagcagga gcagcaggtt aaaacccgta ctgccgaaca gaatgcacgt
      781 attgcggctt ttgaagctga acgtcgtcgt gaagcagagc agacgcgaat tctggctgaa
      841 cgacaaattc aggaaacgga gatcgagcgc gagcaggccg tccgctcaag aaaggttgag
      901 gctgaacgtg aagttcgtat taaagagatc gaacagcagc aggtcaccga aatcgccaac
      961 cagacgaaat cgatcgctat tgccgccaaa tcggaacaac agtcccaggc agaagcgcgt
     1021 gctaaccttg cactcgcaga agcagtaagt gcgcaacaaa acgtagaaac cactcgccag
     1081 accgcagaag ccgatcgtgc taaacaagtt gccctaatcg ctgccgcgca ggatgcagaa
     1141 accaaagcgg ttgaactgac cgtgcgggcg aaagcagaaa aagaagccgc agagatgcag
     1201 gcggcggcta tcgttaagtt agccgaagct acacgtaaaa agggtctggc ggaagcagaa
     1261 gcacaacgtg cgctgaacga tgctatcaac gtactttctg atgaacaaac cagccttaaa
     1321 ttcaaactgg ccttgttgca ggcgctacct gcggtaatag aaaaatccgt tgagccgatg
     1381 aaatctattg acggtatcaa gattattcag gtcgatggtc tgaatcgtgg cagcgctgcg
     1441 ggtgatgcaa acacgggtaa tgtggggggc ggaaacctgg cggaacaagc attatcagcc
     1501 gctctctctt accgcacaca ggcaccgctg attgactcct tgctcaatga aattggcgtt
     1561 tcaggcggct cactggcggc attgacttca cccttaacct caacaactcc cgtcgccgaa
     1621 aacgtagaat aaattttcag ctatgaacgt ccctggcaca aaaagccggg gattttttat
     1681 atctgcgttc cgctaaaaac tgcaaatgat caggtcgttg caacgttttg cgtgaccgct
     1741 cggggaaggc aaaactgcct ctgggaaagc gttgcgcgag gtccggcgct catcaacaat
     1801 cggggagtca gcaaagggct gaaacgggaa agcccctccc gaggaagggg ccttacagaa
     1861 ggaaagggtt atgatgaagc tcgtcatcat actgattgtg ttgttactcg taagtttcgc
     1921 agcttattaa cagccaatca gaggggggag aaatcctccc tcacccttgt tcctttactt
     1981 taggttgaaa aaaaatcagc gtcaagaagg tttcattaac acatatccct agcccaaaaa
     2041 gccggggatt ttttatatct gcgttccgct aaaaggtgca aatgctcagg acgttgcagc
     2101 gttttgcgtg accgctcggg gaaggcaaaa ctgcttcagg gaaagcgttg cgcggggtcc
     2161 ggcgctcatc aacaatcggg gagtcagcaa ggggctgaaa cgggaaagcc cctcccgagg
     2221 aaggggcctt atagaaggaa agggttatga tgaagttcgt catcatactg gttgtgttgt
     2281 tactgttaag tttcccgact tactaacaac tgatcagagg ggggagaaat cctcccttac
     2341 ccttgttcct ttactttagg ttgaaaaaac aacagcgtca ataggcctgc ctgctacgaa
     2401 gcgagatctg tgaaccgctt tccggttagc cttttttatc ctgttggatc ttcttgatga
     2461 tgttagtggt cgagcaaccg tcttcgaagt tgagcaccag cacttcgccg ccgttggccc
     2521 agacttcttt actcccggcg atctcttctg gtttatagtc gccgcccttc accagtaaat
     2581 ctggcaggat cccggcgatc aagcgctgcg gcgtgtcttc ttcaaacgac accacccagt
     2641 cgaccgcttc cagtgcgccc agcacaatca tacgctgttc gagcgggtta acggggcggg
     2701 aatccccttt cagccgtttg gtggaggcat cgctgttcac agcgacaatc agacggtcac
     2761 ccagtttgcg agcatttgcc agataggaga cgtgcccggc atgcaggatg tcaaagacac
     2821 cgttggtcat taccaccttt tcgccgcgtt tacgcgctgc cgctacggcc agcttcagtt
     2881 cctcttcggt catcacgcca aagccggtat ctgcgcgccc acgcactgca ttttccagct
     2941 cgatcggcga aaccgtggag gttcccagtt tgccgaccac cacgccagcc gccgcattgg
     3001 caaagaagca ggcttcttcc agcgaattac ccgctgccag cgttgccgcc aggacgccaa
     3061 tcaccgtgtc gcccgcaccg gtaacgtcat acacttcctg cgcctgggtt ggcatatgca
     3121 gcggcgcttt acccggttgc agcagcgaca taccctgttc ggaacgggtc actaacagag
     3181 ccgagagttc gtaatcggca atcagtttca tgccgcgctc aacaatctct tcttcggtct
     3241 tacatttacc gacaacagct tcaaattccg agagattcgg cgttaacagc gtagcgccgc
     3301 ggtagcgctc aaaatcggta ccttttggat caatcagcac cggaacgccc gctttacgcg
     3361 ccagttggat catctgctgt acgcttgcca gcgcaccttt ggcgtagtca gaaagcacca
     3421 gcgcgccaat cgaactcagc gcctgattaa tccgctcgtg cagcggctgc ggatcaacgc
     3481 cttcgaaacc ttcttcgaaa tccagacgga tcagctgttg gttgcgggaa agtacccgta
     3541 atttggtgat ggtcggatgc gtcggtacag aaacgaagtc gcatttgacg ttgacgtcgg
     3601 ccagagattt actcagcgcg cgcgctgcat cgtcaatgcc cgtcaacccg accaggcgtg
     3661 cattagcacc gagagaagcg atattcatcg ccacgttagc cgcgccgccc ggacgttctt
     3721 cgatggtatt cactttaacc acgggcaccg gcgcttccgg cgagatacgg ctggtgggac
     3781 cgtaccagta acgatccagc attacatcac caaccaccat cactcctgca cgttcaaact
     3841 ctgacagcgt tactttcatt cctgtctcct gagagattca aaatttgcgc gcgataatac
     3901 catacttcat tcttccacca gccacttctg ccagcttgcc cgcaccagat cacgctctgc
     3961 ggtgaagcaa tcctccgaca catggcccgg caattcctgt aatgccagat gatgaagttc
     4021 atcgcgcagc gtcgtgtaag cacgggtcag cgccatcgct tcctgctctt ccataatgtc
     4081 gttttgcgcc agcagctcca gaatacgcac gttgtctgac cagcgcgtta acttcggttt
     4141 ttcatgagcg tagcgcaaca ccagatattg ggtaataaat tcgatatcgg taattccgcc
     4201 ttcatcggct ttgatatcaa agcgattacg atgtttattg ccgagatgag cgcgcatttt
     4261 ctcgcgcatt tcccgcactt ccgtttgcag agttttacct tcacgcggca gcatcataat
     4321 ctcgcgacgc actgcgtcaa agtgcgcggt gagctgcgga tcgccgtaca ctacacgcgc
     4381 acgcaccagc gcctgatgct cccacgtcca ggcctcgttt ttctgataat cagcaaatgc
     4441 ttctgcggat gtcaccagca ttcccgccgc cccggacgga cgcagtcgtg catccacttc
     4501 atacaaaatg ccggaagagg ttcgcgtatt gaacagatgc ataatgcgtt gcgccagacg
     4561 cagataaaac tgtcgcccgt cgatttcccg ctcgccgtca gtcatcgcat ccattgggca
     4621 atcatggagg aagatcaggt caagatcgga actgtagcct aactcccagc cgcccagctt
     4681 gccgtagccg accaccgcaa aaccacggcc ttcgcgatcg ttgaggtgat ttggcttacc
     4741 gtagcgggcc accatttgaa cccacgcctg ctgaacgacg gcatctatca tagcctctgc
     4801 cagccaggtt aagtgatcgc tcactttcat caccggtagc gtaccagcga tatccgctgc
     4861 ggcgatgcgt aacagctgcg cctgtttgaa ctgacgcagc gcctcaagct gttgctcttc
     4921 gtcatcttcc ggcacgcgca gcaaatactg gcgcaactca tcgcggtagg catcggtcgc
     4981 cgtcggctgg taaagagtgt ttggatcgag caattcatcc agcaataatg gataacgcgc
     5041 cagctggctg gcaatcatcg gcgatgcggc acacagagaa atcaaatgtt taagcgccgc
     5101 agggaattca ctgagcaatt ctaaataggt ggtgcgggta acaatcccca ccagcaaagc
     5161 ggtaatgcgc gacagcgtaa cggcagcgtc ttcacgcgca cagacatcac ttagcagatg
     5221 tggcatcaga tggtcgagca cctgacgtcc tcgcggcccg atagtacgct tatccagctc
     5281 tttgcggaaa tcagcaatca gcgttaggac ctgtttgcga tcatcctcgc taagatgcgc
     5341 cagcactggc gtagtgtcat cttcctgcaa cgcatcctgc cacagctcac gccactgttc
     5401 cgacagcgac tcttcctgag tttcactttc gtcgtcgcca atcaattcat taaacacccg
     5461 gcgcacattg gtcatatgtg cggtcagcgc cccggtcagt tgcggccagt cagcaaagtc
     5521 catcgcccac gccagccgcg cacggttaag ctcatcagaa ggaagcgttt gggtttgttc
     5581 gtcgttaatg ctttgcagca ggttttccag acgccgcagg aacagatatg ccactcgcaa
     5641 ttgttcagca tcgttttcag aaagcagatg cagcgcggca atgacgctga gcgttggcag
     5701 taaagagcgc gactgcagcg acggttcgcg cccaccgcga atgagctgga acacctgaac
     5761 gataaattca atttcgcgaa tgccgcctgc gccgagtttg atattgtcgg tcaaaccacg
     5821 tcgacgcact tcacgggcaa tcatcccttt catgttgcgc agcgactgaa tcacgctgaa
     5881 atcgatgtaa cgacggaaaa caaacgggcg cagcatcgca cgcaactcgt tagcatagac
     5941 gccttcgcta tcgcccataa tccgcgcctt gaccatcgcg taacgctccc agtcgcgccc
     6001 ctgctcctgg taataatcct ccaacgcggc aaagctcagc accagcgggc cactttcgcc
     6061 aaacggacgc agccgcatat ccacgcgata gacgaagcca tcctgcgttg gttgatccaa
     6121 cactttaatc agccgctgcc ccatgcgggt aaaaaactgc gcgttatcca gttcccggcg
     6181 tccgccctgc gtacaaccat gttccggcca ggcaaaaatc agatcgatat ctgaggagaa
     6241 attcagttcc ccaccgccca gcttacccat gcctaaaatc agcagcggtt gcgcttcgcc
     6301 ctgcgcattg cacggcgttc cccactcgcg gcagcaagcg tcatacagcc agtcacgcgc
     6361 ggcgacaatc agcgtctccg ccagatggct gagctgctgc aatatgctct cttcagtaac
     6421 caaagccagc gtttgcgccc aggcgatgcg caccataatg cgccgccgga atagccgcag
     6481 ctcgcgcatt aacccggctt cgtcactcac attgctgagc gcctcctgca accatgccgc
     6541 gtaatgctgc cattcatcag cctgcggcga ttggctttcc agttccgtca gccactctgg
     6601 atgcgcaatc acgctgtcct gcacaaaatc actgaaagta agtactgact tcgcctgtgc
     6661 gctaagtgat ttctcggcta aaggctctgg cagccgctca acaacggtct gccagtactg
     6721 ctgtaacggt gaagagagcg gcttcataag cgattttatc cttgcctgat taacgttttc
     6781 cgctgtgcaa ccagaacggt tcctgattgt ttgcctcatt acggaaatgt tcaatttcaa
     6841 tgcgttgccc ggtcgcaata gcgtgacgca gcccctgcca gttctccagc caggcttgcg
     6901 cgacgacagg atcatagtaa cccgccagca acagtattga gtcaatatca cgcgtcagac
     6961 gtggcaactg gtcacggtag cgatcgccca acggctggca gaaaacgctt ttcagttcag
     7021 cggcatggcg ggaaagatgg atatcggcaa agcgtttgaa ggaatcgctc attttactct
     7081 gcgctttggc atctaaaaac ggctgccagg ctttgcttac cagccattcg gtcaacgcca
     7141 gcttcgccat tgccgttttg gtagagtaga ccgccgtcac ggcagaaacc gcagaagcga
     7201 tggtcgcttc gcattgagtc agcaaatcgc gtaagtgagt gctcgctttg cgcggcacaa
     7261 taccaccgaa cagcatcagt gtatgacgga ccagaccaat ggctgccagc acctgttctt
     7321 tcgccgcatc gttgccgcgt acccacagtt cttcatgata ctgccattgc gctaacgcca
     7381 gctcgagcgc cgcttccagc ccctgttcca cataggcttt tgccgcaaca tgcaaaatgg
     7441 tggtcggttt gatttcacgc gccggattgc cctgcgccag atgatagcca cgcgccgctt
     7501 tgctcaggct gccctggcgt aatccggttt gcgataccag ttggttcgcc agtttcagca
     7561 ccgcgcgcgt gtcgccgcta agcagttcca gttccagctc acagataggt tcagcaaatt
     7621 cccccgcttt cacctccccc tgatcgagag cgatttcaat tcggctgtca tcgaccgcca
     7681 ccagccattt ttcgcgataa aaatcggtgc tgaacagcgg ttgtacgcgg gaagcgagat
     7741 cggcgggcaa ttcgccgttc ggccagactt ccgtcggtaa ctgcgccagg tcgagcgtcg
     7801 gttcgctcaa cgccacgtta tattccgggc gctggtgtaa gccgcctgtc actcttcctg
     7861 caactttcat ggtcatctca tagcgaccgt tttcgccacg aatacgtaag cccatatcgt
     7921 gcccacgcag ccagttatcc ggcgtttcgt agtaaatatt cagcaactgc acggggtcat
     7981 ggtgctcgcc gcccagcgta ttgagatggt cacgcaacgc ctcaacggca ctgtgattaa
     8041 caataaactt taattcgatt tcctgagcca tggcctttac ttatcggtta tgtcacataa
     8101 gtgacgatga acggcgaact taatgcgatc ttttttgtca gtagatagta ttttgcgcca
     8161 aattgccatg caacgggcaa tttgacgggc gtaaaagttt gaagcagtgg caagacctga
     8221 cacagaggat tccgattgat gtcgaatgct ttgcgtagcg acactgatgc cactactatc
     8281 gttccacttt ccatgacaat aacgacagcc tgatgccaaa attacgcctg atcggattaa
     8341 ctttactcgc acttagcgcc actgccgtct cacacgctga agaaacgcgc tatgtttctg
     8401 atgaactgaa tacctgggtc cgtagcggtc cgggagatca ttatcgcctc gtgggcacgg
     8461 ttaacgccgg cgaggaagtg accttattac aaactgacgc caacaccaat tatgcccagg
     8521 tgaaagacag ctctggccgt accgcctgga tcccgttgaa acaacttagc actgagccaa
     8581 gcctgcgctc ccgtgtgcca gatctggaaa atcaggtcaa aaccctgacc gataaactca
     8641 ccaatatcga taacacctgg aatcagcgca ccgcagaaat gcagcaaaaa gtggcgcaga
     8701 gcgacagcgt gatcaacgga ttaaaagaag aaaatcagaa attgaaaaac gagctgattg
     8761 tcgcgcagaa aaaggtcgat gccgccagcg tacagctgga tgacaaacag cgcaccatca
     8821 tcatgcaatg gtttatgtat ggcggtggcg tgctggggct tggcttgctg ctcggtctgg
     8881 tactgccaca cctgatccca acgcgcaaac gcaaagagcg ctggatgaac taaatcgcct
     8941 tcttcgttgc actgtcttat tattaggaat tgtgtatttg acgaagggat gatggcgtga
     9001 agatttatct ggtcggtggt gctgttcggg atgcattgtt agggctaccg gtcaaagaca
     9061 gagattgggt ggtggtcggc agtacgccac aggagatgct cgacgcgggc taccagcagg
     9121 taggccgcga ttttcctgtg tttctgcatc cgcaaacgca tgaagagtat gcgctggcac
     9181 gtaccgaacg gaaatccggt tccggttaca ccggttttac ttgctatgcc gcaccggatg
     9241 tcacgctgga agatgatctt aagcgtcgcg atctgaccat taatgcgctg gcccaggacg
     9301 ataacggtga gattatcgac ccgtacaacg gtctgggcga tctgcaaaat cgtctgttgc
     9361 gccatgtttc ccccgctttt ggcgaagatc cgttacgcgt tctgcgcgtg gcgcgttttg
     9421 ctgcgcgtta tgcccacctc ggttttcgta ttgccgatga aactctgacg ctgatgcgcg
     9481 agatgaccca tgcgggtgaa ctggaacacc tgacgcctga acgggtatgg aaagagacgg
     9541 aaagcgccct caccacccgc aatccccagg tgttcttcca ggtactgcgc gattgcggtg
     9601 cacttcgcgt tttattcccg gaaattgacg ccctgtttgg cgttccggcc cctgccaggt
     9661 ggcatccgga aatcgacacc ggtattcata ccttaatgac gctctcaatg gcggcgatgc
     9721 tgagtccgca ggttgatgtc cgtttcgcaa ctttatgcca cgatctcggt aaaggactga
     9781 cgccgccaga actttggccg cgtcatcatg ggcatggccc ggcgggtgtt aagttagtgg
     9841 aacaactatg ccagcgtctg cgcgtgccaa atgaaattcg cgatttagcc agactggtgg
     9901 ctgagtttca cgatctcatc cacaccttcc caatgctgaa cccaaaaacc atcgtcaaat
     9961 tatttgattc catcgatgcc tggcgtaaac cgcagcgtgt cgagcaactg gcgctgacca
    10021 gcgaggctga cgtacgcggc agaaccggtt ttgaatcagc ggactacccg caaggccgtt
    10081 ggttgcgcga agcctgggaa gtggcgcagt cagtgccgac aaaagcggtc gttgaagcgg
    10141 gatttaaagg tgtggagatt cgcgaggagc tgacccgacg gcggattgcg gcggtagcca
    10201 gttggaagga acaacgttgc ccaaagcctg actgagtata aaactaaacg ttaacacgaa
    10261 ccatcccctc gccccttcgg ggagagggtg aggggaacag accagcaccg gtgcgaacaa
    10321 tcacccccac cccggccctc tccctacaga gcgagggaga aaaccaggcc tgctcccgaa
    10381 ccttttctca atcaaaagaa cacgacgtac accgcagccg ccacaataaa gcgataaatg
    10441 gcgaacggga taaacgaaat gcgcttaatc aattgcagga aggttttaat cgctatcagc
    10501 gccaccacaa aagcggtgat aaacccaacg gcaaacatcg ggatatcgcc ggttgtcagg
    10561 aagccccagc ttttgtagag atcgagcgcc gttgcgccca tcatcatcgg taccgccagc
    10621 aggaacgaga actcggaagc agcgtaacgg ctcacgccca tcagcatccc acctgaaatg
    10681 gtggccccgg aacgggagaa acccggccac agcgccagac actggaaaca gccaatcata
    10741 aacgcctgac gataggtcat atcatcaaga cccggcgcac gcggctcttt cggcttcagg
    10801 cattcggcgg caatcagcaa caaaccgcca acgaccagtg catacatcac atttatcggg
    10861 ttaaacaatg acttaatcgt gtcgtggaac aacagcccca gtaccaccgc cggaatcatc
    10921 cccagcaaaa tgtggatcag cgttaaacga cctttgcttt ctccttcgtg cggcaacggg
    10981 cggccaaagt ggatgccaat caggccaaac agacgccgcc agaacatcac tactaccgcc
    11041 agaattgatc ctaactggat cacaacttca aaggttttcg ccgtgtcgcc ctcgaacccc
    11101 aacaagtgac cgacaataat catatggccc gtgctggata ccggcagaaa ttctgtcaat
    11161 ccttcgacca cacccaatat tgccgctatc agcagcgagt gcatatcgct catcaataaa
    11221 cccctaaatt attaaaatgt accgcttgtc cggactactg cagtaagacc aggttataac
    11281 cgtttggttt aacagctgta aaattaatta ttttctttca gattattgcc acgctcaatg
    11341 attacaccaa cattcgccgc ccgcgccact gcgcctggct tgctgagttt gatgcgcacc
    11401 cacggcgagt tgaagcgtgc taacagcagc tccgccacct cttcagccac gcgttccacc
    11461 agcgcaaaac gcgccccctc gacgtggctg accaccgttt ctgcaatgtc agcgtaactg
    11521 aggcaatccg ccacatcatc acttttcgcc gctttacggt tatcccatgc catttcgata
    11581 tcgaacacta acttctgttc gatggtctgt tcccagtcgt aaacaccaat agtggtgatt
    11641 accgaaagtt gctctataaa tacaatatcc atcacgtcct gcctgctttt tggctaaccg
    11701 gataccactt ccggcgaaat gtgcgtatta tccacagatt catcgttgaa cacgaatttt
    11761 caaaacggaa cagcttatga gtgcaatcgc gcctggaatg atcctcatcg cgtacctctg
    11821 cggctccatt tccagtgcca ttctggtttg ccgcttgtgt gggctgcccg atccgcgaac
    11881 cagcggctcc ggcaatccag gcgcaaccaa tgtgttacgt atcggtggca agggagcagc
    11941 cgtagcagta ctgattttcg acgttctgaa aggaatgttg cccgtctggg gcgcgtatga
    12001 attaggtgtc agccccttct ggctaggctt aattgccatc gccgcctgtc ttggacacat
    12061 ctggcccgtt ttcttcggat ttaaaggagg aaaaggcgtt gctaccgctt ttggtgccat
    12121 cgcacccatt ggctgggatc tcaccggagt aatggcggga acctggttac tgaccgtgct
    12181 attgagcgga tactcgtcgc tgggagcgat tgtcagtgca ctgattgctc cgttttatgt
    12241 ctggtggttt aagccacaat tcaccttccc agtttcgatg ctctcttgcc tgatcctgct
    12301 gcgtcatcat gacaacatcc agcgtctgtg gcgtcgtcag gagacaaaaa tctggacgaa
    12361 attcaaaaga aagcgcgaaa aggatcccga gtgatttctg gtggatctac atgacctgat
    12421 agccttcatc gggcttgccc agccgttgct ggcaccatgc cgccagaaat tccacgcaga
    12481 cacgtaattt catgctgcga tagagcggct cccggtaaac agcccagata ttggcgcttt
    12541 gtgcatactc cggcaatact tgcaccagtt tgccactctc cagaaacggc aacacatccc
    12601 actcggaacg cagcataatc cctttgccct ccagcgccca ttgcaacact atctcgccgc
    12661 tattggagga aagatgcccg ctaaccttca ccgatttttt ctcctgaccg ttccccaact
    12721 cccatattcc gtgggtcata tcgcgctctt tggtcaccag acagtcatga cga
//
LOCUS       DADLFA010000119        12713 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000119 DADLFA010000000
VERSION     DADLFA010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12713
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.119"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..619)
                     /locus_tag="JQH65_002803"
     CDS             complement(<1..619)
                     /locus_tag="JQH65_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HAY6140168.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSR"
     gene            complement(1007..1753)
                     /gene="cutC"
                     /locus_tag="JQH65_002804"
     CDS             complement(1007..1753)
                     /gene="cutC"
                     /locus_tag="JQH65_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAY6140169.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQWVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLNTLSNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(1767..2333)
                     /locus_tag="JQH65_002805"
     CDS             complement(1767..2333)
                     /locus_tag="JQH65_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAY6140170.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEYERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            2549..4282
                     /gene="argS"
                     /locus_tag="JQH65_002806"
     CDS             2549..4282
                     /gene="argS"
                     /locus_tag="JQH65_002806"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAY6140171.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWHKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            4459..4947
                     /locus_tag="JQH65_002807"
     CDS             4459..4947
                     /locus_tag="JQH65_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HAY6140172.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDIEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(5067..5459)
                     /locus_tag="JQH65_002808"
     CDS             complement(5067..5459)
                     /locus_tag="JQH65_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HAY6140173.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(5459..7537)
                     /gene="flhA"
                     /locus_tag="JQH65_002809"
     CDS             complement(5459..7537)
                     /gene="flhA"
                     /locus_tag="JQH65_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HAY6140174.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGCEQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(7530..8633)
                     /gene="flhB"
                     /locus_tag="JQH65_002810"
                     /pseudo
     CDS             complement(7530..8633)
                     /gene="flhB"
                     /locus_tag="JQH65_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
     gene            complement(8716..9360)
                     /gene="cheZ"
                     /locus_tag="JQH65_002811"
     CDS             complement(8716..9360)
                     /gene="cheZ"
                     /locus_tag="JQH65_002811"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HAY6140175.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMIAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(9371..9760)
                     /gene="cheY"
                     /locus_tag="JQH65_002812"
     CDS             complement(9371..9760)
                     /gene="cheY"
                     /locus_tag="JQH65_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheY"
                     /protein_id="HAY6140176.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFDKLDM"
     gene            complement(9775..10713)
                     /locus_tag="JQH65_002813"
     CDS             complement(9775..10713)
                     /locus_tag="JQH65_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HAY6140177.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPVGMLAMRQAGAWTLAQNEASCVVFG
                     MPREAINMGGVCEVVDLSQVSQQMLAKISAGQAIRI"
     gene            complement(10716..11528)
                     /gene="cheR"
                     /locus_tag="JQH65_002814"
     CDS             complement(10716..11528)
                     /gene="cheR"
                     /locus_tag="JQH65_002814"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HAY6140178.1"
                     /translation="MRLPGCSRYSFRRISQLIYQRAGIVLADHKRDMVYNRLVRRLRS
                     LGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHHFPLLADHARRRSGEYR
                     VWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVLEKARSGIYRHEELKNL
                     TPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQYTVPGPFDAIFCRNVMI
                     YFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLRGQTVYALSKD"
     gene            complement(11666..12232)
                     /gene="flhC"
                     /locus_tag="JQH65_002815"
     CDS             complement(11666..12232)
                     /gene="flhC"
                     /locus_tag="JQH65_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HAY6140179.1"
                     /translation="MSEKSIVQEARDIELITLGARLQMLESETQLSRGRLIKLYKELR
                     GSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLYLEQC
                     PQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLCQPPS
                     RAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(12235..12585)
                     /gene="flhD"
                     /locus_tag="JQH65_002816"
     CDS             complement(12235..12585)
                     /gene="flhD"
                     /locus_tag="JQH65_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HAY6140180.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVNDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         3211 a         3433 c         3015 g         3054 t
ORIGIN      
        1 tgcgagaagt tttgaaagga taaatatgtg cgccattagt cactgaagta gcggcctgac
       61 tttccagctc atgcgccgcg ttgtcatcca tttttatttc tggcggaaaa tgggcaatgc
      121 ctttatgaca atcgatacag gtttcgccgc ccttttgtgc tttgttatgc attttctgcg
      181 cagattcact ttgcgaggca atatccatgg catcaaaaga atggcaacta cggcacgttg
      241 cagagtcagt tgcttttaat tctttccata ctgtttcggc catttcctgg cgatgagttt
      301 cgaacttatc gtcactgtct attttgccgc taacaaattc atgataaata tctttagatg
      361 cttttaattt agcaaataaa taatccatcc ctgactttgg aatatggcaa tctgcacatt
      421 ccgcacgtat ccctttctgg ttcgaaaagt ggacagttcc ctgatattcc tcaaaaggtt
      481 tactcatcga gtggcaggaa aggcaaaatg ctgtatccga cgttttatgt aaggcttttt
      541 gcgccagcaa taacccgccg ccaccaacca caaccgctat cagcaaaaac aataacccaa
      601 tgcgtttttt ccctcgcata gattcctcta aataaattcc agaacaccga aatcattgga
      661 taattaaata cacaacgaat taaccgcttg cataactcgg cacaacactg cctgaaataa
      721 tcgataaaga atatgattta ttacaatgta atcattaatt gctaaggaat aaccctgttg
      781 ttatttagaa tatatatttg tcagttttag caatttaaac cccataatta atgtgaatat
      841 ataaaatatt tttagcacaa cgctatgttc actgtcaccc gctcaacaga tcacggttat
      901 cgtacgtttt ttatactgct cagggataaa aaaaggcccc tgtcgaaatt gcaggggcct
      961 ggtacgagca agcatcatat tgggcgacat gatgcaacgg taaaaatcat ttggcctgat
     1021 ggcgttcaat gattcctttc atttcagcaa ccgccgcccc gtctacgaca taacgcgaat
     1081 actcgtccgc gtgtgcatct gatgacatgg acaatccttg attacgataa cgcatcggtg
     1141 aagcttgcca cgctcccgcg gagctatgga cttccagcac tccggcatcg aggaagtggt
     1201 gcaggttttc tgcacggact cctgctccgg ccataatgat tggagcatca cgatgggcaa
     1261 taagttccat aatttttgat aaaccttgca gtgcatctga tttttgccct gatgtcagca
     1321 cccgggcgat gcccaattcc gcaagattac tgagtgtatt taaagggtta gcgcacatat
     1381 cgaaggcgcg atgaaaagtc actgccagcg gaccggcagc agccattatt ttttccattc
     1441 gtggcatatc gacattcccg tcaacatcga gcacgcccgt caccagtcca ggaaaaccta
     1501 attcgcggac tgtgcgcaca tcctcaagaa tggcggcaaa ctcaccgtcg ctgtagcaaa
     1561 aatcaccacc gcgtgggcgg ataatcggat gcacagggat cgtcacccac tggcgcacgg
     1621 atttcagtac acccaacgac ggcgttaagc ccccctcttt tggggctgcg cataattcaa
     1681 ctctgtctgc gccgttttgc tgcgccgtta gtgcacattc catgctgtaa cagcaaattt
     1741 ccagtaatgc catttttact ccttaattac gccgactgct cgctggcaac gatctcttca
     1801 atggaccacg gatgaaactt aatagttgtt ttgccatcgg tgaccgccag cgtcggattc
     1861 ggtaatcgct catactcacc ttttggcgaa ctggttttca ctgagatccc cggtaggctg
     1921 agtccttcat cagaaagcaa tgccagcgca cgggcattca gcgtttctgg atcacccggc
     1981 agaacgattt caatatgttc ccaaccttcg tgtgggtaac gtttttcccc gggccacggt
     2041 agctccacaa tggaaaactg ccagtgcgca acctgtaccg gttcatgcaa tttaaacaga
     2101 caaatcggtc tgccattgat catattttct gacaaaagct cgccacactg ttcaaaaccg
     2161 cgacgccagc gctcagcggt gacgttttga tggcaacgca aagaaatgtg atcggcagtc
     2221 agcggagtga tatccaaccc cagacggcgg gagagttcat ctaatgcgtg ggtaaatcgc
     2281 ggtaaatccg atgcaatatc ctgcagctcg tcgatagatt gccagttcgc cataatcact
     2341 cttcgtcttt cagtaaaagc gttaatttac cctgttgccc tgcgccaacc aaccgctgat
     2401 ttcacgccgc ttctgatgca atagtgaaaa cggcaatacg ccacgcgcac gttgctgacg
     2461 aaaacagcca tttgcagtat actcccgccc taatttcttt aactggtgcg ggcaattttt
     2521 gctcgcttca tcaatgtaag gtattccggt gaatattcag gctcttctct cagaaaaagt
     2581 ccgtcaggcc atgattgcgg caggcgcgcc tgcggattgc gaaccgcagg ttcgtcagtc
     2641 agcaaaagtt cagttcggcg actatcaggc taacggcatg atggcagttg ctaaaaaact
     2701 gggtatggca ccgcgacaat tagcagagca ggtgctgact catctggatc ttaacggtat
     2761 cgccagcaaa gttgagatcg ccggtccagg ctttatcaac attttccttg atccggcatt
     2821 cctggctgaa catgttcagc aggcgctggc gtccgatcgt ctcggtgttt ctacgccaga
     2881 aaaacagacc attgtggttg actactctgc gccaaacgtg gcgaaagaga tgcatgtcgg
     2941 tcacctgcgc tctaccatta ttggtgacgc agcagtgcgt actctggagt tcctcggtca
     3001 caaagtgatt cgcgcaaacc acgtcggcga ctggggcact cagttcggta tgctgattgc
     3061 atggctggaa aagcagcagc aggaaaacgc cggtgaaatg gagctggctg accttgaagg
     3121 tttctaccgc gatgcgaaaa agcattacga cgaagatgaa gagtttgccg agcgtgcacg
     3181 taactacgtg gtaaaactgc aaagcggtga cgaatatttc cgcgagatgt ggcacaaact
     3241 ggtcgacatc accatgacgc agaaccagat cacctacgat cgtctcaacg tgacgctgac
     3301 ccgtgatgac gtgatgggcg aaagcctcta caacccaatg ctgccaggaa ttgtggcgga
     3361 tctcaaagcc aaaggtctgg cagtagaaag cgaaggggcg accgtcgtat tccttgatga
     3421 gtttaaaaac aaggaaggcg aaccgatggg cgtgatcatt cagaagaaag atggcggcta
     3481 tctctacacc accactgata tcgcctgtgc gaaatatcgt tatgaaacac tgcatgccga
     3541 tcgcgtgctg tattacatcg actcccgtca gcatcaacac ctgatgcagg catgggcgat
     3601 cgtccgtaaa gcaggctatg taccggaatc cgtaccgctg gaacaccaca tgttcggcat
     3661 gatgctgggt aaagacggca aaccgttcaa aacccgcgcg ggtggtacag tgaaactggc
     3721 cgatctgctg gatgaagcct tggaacgtgc acgccgtctg gtggcagaaa agaacccgga
     3781 tatgccagct gacgagctgg aaaaactggc taacgcggtt ggtattggag cggtgaaata
     3841 tgcggatctc tccaaaaacc gcaccacgga ctacatcttc gactgggaca acatgctggc
     3901 gtttgagggt aataccgcgc catacatgca gtatgcttac acgcgtgtat tgtccgtgtt
     3961 ccgtaaggca gaaattaacg aagagcaact ggctgcagct ccggtaatca tccgtgaaga
     4021 tcgtgaagcg caactggcag ctcgcctgct gcagtttgaa gaaaccctca ccgtggttgc
     4081 ccgtgaaggc acgccgcatg tgatgtgtgc ttacctgtac gatctggctg gtctgttctc
     4141 tggcttctac gagcactgcc cgatcctcag cgcagaaaac gaagaagtgc gtaacagccg
     4201 tctgaaactg gcacaactga cagcgaagac gctaaagctg ggtctggata cgctgggtat
     4261 tgagacagta gagcgtatgt aatcgatttt tcgtgagagt aaagcctgat cagggttacc
     4321 tgatcaggct tttttattgc catctaaatg tattctgaaa atggacatgc cattgttttc
     4381 tgactgttgg ataagaggcc agaagcgtaa tatccggccc cagggaaacg ataacggttg
     4441 aatttaagga ataccgcagt gtttaaattt cttgtattaa cattaggcat tatctcttgc
     4501 caggcttacg cagaagatac ggttatagta aacgaccatg acatttcagc catcaaagat
     4561 tgttggcaaa aaaattcaga tgatgatact gacattaacg tgatcaaatc atgcctgcga
     4621 caagaataca atctcgtcga tgcgcaatta aataaagcct atggtgaagc ttatcgttat
     4681 atagaacaag tgccacgcac aggtgtaaaa aaacctgata tcgaacaact taacttgctt
     4741 aaaaaatcac agcgagcctg gctggatttt agggacaaag aatgtgaatt aatcctttca
     4801 aatgaggacg ttcaggattt aagtgaccct tattctgaat cagaatggct ctcatgtatg
     4861 atcatacaga ccaatacgcg aactcgccag ttgcagctat accgtaactc tgaagatttt
     4921 tatccaagcc ctttgacaag aggataattc acatcttttt ggcatgtttt gttgcaagct
     4981 attcctgata aataattgca acaagacatc gagccttttt cactgagtta ttaaacatac
     5041 tcgcgagcgc gtaatttttt tgtccttcag cgataattca caatcacttc attacgttgt
     5101 acctttagcg gtggaattaa ccgcccaccg cctggcactt cccagataaa tcgcaacggt
     5161 tctgctgccg ctataccgga aaaggccacg gtggttccgc tctgcccctc taattcgaca
     5221 caacgagatt gcgaacacaa gcgaacccgc agtcctgaag gtgtcgggcc gataagctga
     5281 taacgccacg ctaccagcgt catcaatcct gaagcaggtt gtcgcgtaga aagaggcgca
     5341 gacgacatcg actcaccgcg atgatttagc gtaattccta cactacttgc ctgccacatc
     5401 ccctccccgg cggcttgcac cagcagcgga aacaataata ttgctaataa ggttctcatt
     5461 atttgccgcc aattgtcgcc gtcatgcgga tatgtcggtt atcagacagt tccagattcg
     5521 acaggaccac taactgcggc aagctgcggc gcaggaagcg agacaataat ggtcgcagcg
     5581 cgtggttcac caacaatact ggcggcgcac ccagcatctc ctgacgggat agcgcttcct
     5641 gagtttgcgc cagtaaacga tccgccagcc ctggctccag tcctcccccg ccctgcagcg
     5701 cctgtagtaa caaacgttcc agcggtgtat cgaggccaat aacatggact tcatctttgc
     5761 caggaaacca ctgctgggta atcgcccgtc ccaacgccac gcgcacgacg gcggttaatt
     5821 catgtggatc gctttggatg ggcgcatgtt ccgccagcgt ttcgagaatg gtgcgcatat
     5881 cgcgaatcgg tactttttca tcgaggagat tttgcagcac tttatgcagt gtggtgagcg
     5941 tgacgacgcc aggaacgaga tcttccgtca gctttggcat ctcctgggcg acgcgatcca
     6001 acagctgttg cgcctcctga cgaccaaaca gctctgcggc atgctggcta atgaggtggt
     6061 taagatgcgt tgctaccacc gtgctggcct caaccactgt gtacccctga atctgcgcct
     6121 gttcttttag cgcactttca atccagatag cattcaggcc aaatgccgga tcgacggtcg
     6181 cctcaccagg taacgtcccg gcagcggttc cagggttaat cgccaaccag cgccccggat
     6241 aagcatcacc actgccaatc tccacgcctt tcatcaaaat gcgatagcgg gcaggttgca
     6301 gatccatatt gtcgcgaatg tgcaccactg gcggcagaaa tcccatctcc tgggcaaatt
     6361 tcttgcggat actgcgtata cggcccaaca actcaccatc ctgctggaaa tcgaccatcg
     6421 ggatcagtcg ataacccact tccattccca gagaatcttc cagttgtaca tcgttccacg
     6481 tcgcttcgac aacggtatta ttctctgcca tttttaccgg tttgggttcg gcaggcgctt
     6541 tttgttcgca tccgcgtatc caccaggcca gccccagcaa tccggcagtg aacagcaaaa
     6601 ataccaggtt cggcattcca ggcaccaagc cgagtaaacc gagcacggcg gcgcttaaca
     6661 acataacgct tgggttactg aaaagctgat tcaccatctg ctcgccaaca tcctgatcgg
     6721 tgctgacacg cgtaacgatg acccccgcgg cggtagaaat caccagcgcc ggaatttgtg
     6781 ccaccagacc gtcgccaatg gtcaatagcg tataactttc cgccgcgtgt cccatgctca
     6841 tgccatgttg cagcacgccg accagcaacc cgccgacaac gttaatgacc atgatgagga
     6901 tcccggcgat ggcatcgccg cgaacaaact tacttgcccc gtccattgag ccgtaaaaat
     6961 cggcttcctg agtcacttcg gagcggcgtt ttttcgcctc atcttcacca atcaatccag
     7021 cgttaaggtc ggcgtcaatc gccatctgct tacccggcat accatcgaga acaaagcgcg
     7081 cacccacttc tgcgatacgc ccggcacctt tggtaatgac cataaagttg atgatcacga
     7141 gaatgacaaa caccacgata cctatagcga aattgccacc aacgaggaag tgaccgaacg
     7201 cttcgaccac cttccctgcc gccgccgcgc cggtatgccc ttccatcaaa atgatacggg
     7261 ttgaagccac gttaagtgcc agacgcaaca acgtagtaaa caacagaatg gtcggaaacg
     7321 cagcaaactc aagcgtgcgc tgggtaaaca tcgccaccag caacaccatg atcgacaagg
     7381 caatattgaa ggtaaacaac aggtcgagta tgaatgcggg cagtggcagc accatcatcg
     7441 acaagatcaa caggatcaaa atcggtccgg caaggatctg ccattgtgtc gatttcaggt
     7501 ttgcgggcag gcgcagcatc gcggccagat tactcatggg tcggtttctc gttaataaaa
     7561 tccagggctt ccggcaccgg aagatgagta ggttgtacag ggcgctgtcc accagccaga
     7621 cgccagcgtt tcagttgcca gatccaggcc agcacttccg ccaccgcggc gtacagttga
     7681 cccgggattt gttgaccaat ctccgcatgt cgatacagcg ctcgcgccag cggcggcgct
     7741 tcaagcgtcg ggacgttatt ttcagcgcca atttcacgaa tgcgcagcgc gaccagccct
     7801 gcacctttag cgaccacttt cggtgcgctc attttgtttt cgtcatactg caacgctacc
     7861 gaatagtggg tcgggttatt gacaatgaca tccgctttcg gcacatcggc catcatccga
     7921 cgccgtgcgg cagctcgctg catctgacgg atccgtcctt taacatgggg gtcgccttcg
     7981 ctttgtttga actcatcacg aatatcctgc cgcgacattc gcagcttttt caggtggctg
     8041 aagatttgga aaaagacgtc aaatcccacc attggaatga caccaagcac caccagcagt
     8101 gcgcatagcc ctaccaaatc catcgcatta cccatggcgg taatcggaga ctcggccagt
     8161 aagcgcatca tctgcggcca gtgatgccag agaaaaaacc ccgtcacgct gccaaccaag
     8221 atggttttca aaattgcttt aagcaactcc gcgccagtct gtgccgagaa catccgttta
     8281 atgcccggta gcgggttgag tttggaaaac ttcggctgca aggattttcc gctaaatacc
     8341 agccccccca gcatgaccgg agaaatgagc gctaccagca ccacgccgct aatcagcggt
     8401 agcagcgcca gcatggcttc tctgatcagc agaataatct gcccgaggat cagattcggg
     8461 tctttgataa tactgtgatc aaaatgcagc ccagcggaga gcatgcccga caatcgacgg
     8521 gccagcgaca caccgccgaa ccagataaca cacacgccca ccagcaaaat cagcagtgag
     8581 gtcagttcac gggaacgcgg gatttagggc ttttgctgcc acacatcaag catagtgtgc
     8641 gtttgtcgga tgcggcgtca tcgccttatc agaccgcctg atatgacgtg gtcacgccac
     8701 atcaggcaat acaaatcaaa atccaagact atccaacaaa tcgtccacct gatcctgact
     8761 ggctaccaca ccggctttgc tggtatcgac ctgaggtcca ttaagcaaac tctggttttc
     8821 acgttttgga cgcgactcct gttccgggat gttttccaac agcaccatca gcaactggcg
     8881 ttcgatctcc tgaatgacat ccatcatccg cttaataacc tgcccggtga gatcctgaaa
     8941 atcctgcgcc atcatgattt ccagcagttg cgcgttagta aagctggtat gcgcgggtac
     9001 atctgccaga aattgtcgtg tatctgttac cagttcacgg gcgtcggcaa ggtcaatcgg
     9061 atcggcaaac cagtcatccc aacgttgggt taacgctttt gctgatttct ccatttgatc
     9121 ctgatgcggt tgtgacgcct caacactgtt cagcgcccgc tccgcagcct gggcgatcat
     9181 ctgcacaaca tagtacaaac gatcgcgcgc atcggggatg gcttccgccg cttcggcaat
     9241 ggcctgatcc agccccagtt cccgcaaact gtcgcgcagc atacgcgtca ggctgccgat
     9301 gcgcgcaatg atatcgccag ctgaatgctc gtcagcaggt ttgattgatg gttgcatcat
     9361 agtcgcatcc tcacatgtcc agtttatcaa agattttgtt gagtttttcc tccagcgtcg
     9421 cggcggtaaa tggcttcacc acatagccac tggcccccgc ttgcgccgca gcaatgatgt
     9481 tctctttctt cgcttctgca gtcaccatta acactggcaa tgccgacatc gcgccatccg
     9541 cacgaattgt tttcagcaat tccaggccat ccatattggg catgttccag tcggagataa
     9601 caaatccata accgcctgcc tgcaacttat tgagagcgtc gacgccatct tccgcttcct
     9661 caacattatt gaatcccagc tctttcagca ggttacgcac tatgcgtcgc atggtggaaa
     9721 agtcatccac aaccaaaaat ttaagttctt tatccgccat ttcacactcc tgatttaaat
     9781 acgtatcgcc tgtccggcac taatttttgc caacatttgc tggcttacct ggctaagatc
     9841 gaccacttcg cagacaccac ccatattgat ggcctcgcgc ggcatgccga acaccacgca
     9901 acttgcttcg ttttgcgcaa gggtccatgc ccccgcctga cgcatcgcca acattcccac
     9961 cgggccgtcg tgaattttga tttgataatt tgcgccacta cgcgccagtt ccatatgccg
    10021 atcgcccggc gcaatatagg catgccccgg caagacacgt tctccgtctt cggcttcttt
    10081 aaccccgatc tggcaaagct tattaagtct gtcggcaaaa gagcgggtga aaccgggcgg
    10141 catatgttgg gtaattaaca atgccgggct ggaaagcggc aacggttgca gtacgtgacg
    10201 aattgcctca gttccacccg ttgaagcacc aatcgcaatc agtttttcag aactcaacaa
    10261 cggacccgcc ttcagcgttg tcggtgctga caatggctta tgtgctgcaa ggctcgcctt
    10321 tgctgccgta cgcacctttt cagcaatcat ttcgctatac gccagcattc cttcgcgaat
    10381 acccagttgc ggtttggtga caaaatctat tgcccccagc tccagcgcgc gcagcgtgac
    10441 ttctgatcct ttgccggtca aggaagaaac catcacaacg ggcattggac gcaaacgcat
    10501 taatttttcg aggaaatcca gtccgtccat ccgcggcatt tcaacatcca gcgtcagcac
    10561 atcgggattg aatttcttaa tcaagtcacg cgcgaccagc ggatcaggcg cggtcgccac
    10621 catttccatg tcgctatggc tgttgatgat ttctgtcatg atctggcgca tcagtgccga
    10681 atcatcgaca gataacaccc tgattttgct catcgttaat ccttacttag cgcatacacc
    10741 gtctgaccac gcagcgtgaa gcggcgctca aggtggctaa agttttcaga gtgacccgca
    10801 aacagcaatc cgtcgggttt aaggagcgga acaaagcggc gcaaaatctc ctgctgggta
    10861 ttttgatcga agtagatcat gacgttacga cagaagatcg catcaaacgg ccccggcacg
    10921 gtgtactgtt tcgccagtag attcagcggg gcaaaatcaa catagttcgc cagctcctga
    10981 cgcacgcgta ccagcccttc atgcggtccc gtccctcgca tgaaataccg ttgcagttgc
    11041 tgcggcgtca ggtttttcaa ctcttcatgg cgataaatac cgcttctggc tttttccagc
    11101 acttcggtgt cgatatcact ggcaaacact ttccagcgtc cgggcgcggt gcccaatgtg
    11161 tcagccagcg tcatcgcaat gctgtacggc tcttcgccgg ttgaagccgc cgcgctccat
    11221 acgcgatact cgccagaacg gcgacgtgcg tgatccgcga gcagagggaa atgatgtgcc
    11281 tcacggaaaa atgccgtcag attcgtggtc agcgaattga taaacgcctg ccactcaccg
    11341 ctgtgctgat tagattccag caagttcaga taatgaccga aatccgtcag tcccagcgaa
    11401 cgcaaacgac gaaccagtcg gttgtaaacc atgtcgcgtt tatgatcggc caggacgatc
    11461 ccggctcgtt gatagatcaa ttgacttatc cgccgaaaac tgtaccgaga acaaccaggt
    11521 aacctaataa gataagcacg acatcatcct tccactgttg accatgacag gatgttcagt
    11581 cgtcaggcgt taacgcgcga ttggggcaaa aaaaagcagc ggtacgtcgt taccgctgct
    11641 ggcatgttgc gccacaccgt atcagttaaa cagcctgtac tctctgttca tccagcagtt
    11701 gtgggataat atcggcagga ttctgggaaa gtttacgtct ttttactgcc cgggatggcg
    11761 gttgacataa gctgcaggca aagctgccaa caggctggtg agcgtgggta ataaaattgc
    11821 cgccgcagca gttgcagctg gaaagttgca gtaatccact ttcaacaaac cgcaccaatg
    11881 tccaggcacg ggttaatgcc agcagtggtc cttcttctgc ttgtgggcac tgttcaaggt
    11941 ataaacggta ggctttgatc accgcatcga cgccattaca caaacctgtt ttcagtaaaa
    12001 actgccatgc attacagaac atcgaagcat gaacgttttg ttcccaggtc ataaaccagt
    12061 cggttgagaa tggcagcatg cctttcggcg gtgggcttcc gcgcagttct ttataaagtt
    12121 ttatcaggcg tccgcgactt aactgtgttt cgctttccag catctgcaaa cgagcgccca
    12181 gggtgatcaa ttcaatatcc cgcgcttcct gaacaatgct tttttcactc atgatcaggc
    12241 ccttttcttg cgcagcgctt cttcaggctg attaacatca ttcagcaagc gtgttgagag
    12301 catgatgccg gtatgaattt gctggagatc gttaacgcgg gaatcttgcg tcaactgagt
    12361 aatcgtctgg tggctgtcaa aacggaagtg acaaaccagt tgattggttt ctgccagctt
    12421 aaccatttgc ggaagagtca gtgccgctaa cgttgtcgcc atttcttcat ttatgccgag
    12481 acgaaacata gcggacgctt tgtcctgaac aatcaaacgc tgtgcaagta gtaaatatga
    12541 caagttgatg tcataaatgt gtttcagcaa ctcggaggta tgcattattc ccacccagaa
    12601 taaccaactt tatttttatg cggtttcacc gcaccccgtg atgtcgccgg gaagccccgg
    12661 taaaaaataa ttagcattag aatagtgcga taagctgcaa taagcagaac cac
//
LOCUS       DADLFA010000120        12658 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000120 DADLFA010000000
VERSION     DADLFA010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12658
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.120"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(4..324)
                     /gene="chbB"
                     /locus_tag="JQH65_002817"
     CDS             complement(4..324)
                     /gene="chbB"
                     /locus_tag="JQH65_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HAY6140181.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(622..960)
                     /gene="osmE"
                     /locus_tag="JQH65_002818"
     CDS             complement(622..960)
                     /gene="osmE"
                     /locus_tag="JQH65_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HAY6140182.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDADPQAAK"
     gene            1162..1989
                     /gene="nadE"
                     /locus_tag="JQH65_002819"
     CDS             1162..1989
                     /gene="nadE"
                     /locus_tag="JQH65_002819"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAY6140183.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALACPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPQQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2219..3106
                     /gene="cho"
                     /locus_tag="JQH65_002820"
     CDS             2219..3106
                     /gene="cho"
                     /locus_tag="JQH65_002820"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HAY6140184.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMLLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEDATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(3066..3641)
                     /gene="ves"
                     /locus_tag="JQH65_002821"
     CDS             complement(3066..3641)
                     /gene="ves"
                     /locus_tag="JQH65_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HAY6140185.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVVKAKLTEGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3844..4314)
                     /gene="spy"
                     /locus_tag="JQH65_002822"
     CDS             complement(3844..4314)
                     /gene="spy"
                     /locus_tag="JQH65_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HAY6140186.1"
                     /translation="MRKLTALFVASTLALGAANLAHAAAPADAKPMMHHKGKFGPHQD
                     MMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIVSDTFDKAKAEAQIAK
                     MEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATAE"
     gene            complement(4644..5612)
                     /gene="astE"
                     /locus_tag="JQH65_002823"
     CDS             complement(4644..5612)
                     /gene="astE"
                     /locus_tag="JQH65_002823"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HAY6140187.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(5605..6948)
                     /gene="astB"
                     /locus_tag="JQH65_002824"
     CDS             complement(5605..6948)
                     /gene="astB"
                     /locus_tag="JQH65_002824"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HAY6140188.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(6945..8423)
                     /gene="astD"
                     /locus_tag="JQH65_002825"
     CDS             complement(6945..8423)
                     /gene="astD"
                     /locus_tag="JQH65_002825"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HAY6140189.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKLRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGRANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQLETSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     ILMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(8420..9454)
                     /gene="astA"
                     /locus_tag="JQH65_002826"
     CDS             complement(8420..9454)
                     /gene="astA"
                     /locus_tag="JQH65_002826"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HAY6140190.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWHNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAVEKTA"
     gene            complement(9451..10671)
                     /gene="astC"
                     /locus_tag="JQH65_002827"
     CDS             complement(9451..10671)
                     /gene="astC"
                     /locus_tag="JQH65_002827"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HAY6140191.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIMAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGDFPVGALLTTEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            11117..11923
                     /gene="xthA"
                     /locus_tag="JQH65_002828"
     CDS             11117..11923
                     /gene="xthA"
                     /locus_tag="JQH65_002828"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAY6140192.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            12090..>12658
                     /locus_tag="JQH65_002829"
     CDS             12090..>12658
                     /locus_tag="JQH65_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAY6140193.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITL"
BASE COUNT         3075 a         3425 c         3099 g         3059 t
ORIGIN      
        1 aaattaattt gctgcggctt ttttaatcgc tgcaaccgca gccttaagca cgcctaaacc
       61 atcgactttg ccataaagca gcgagtcaat tacttcaacc ggtttgttgg gtaacaaacg
      121 ctggatttcg ggcaacatat aagcaatctg cggccctaat aacacgacat cggcattctg
      181 acctttttca ccagccagtg tttccggaaa tgcttcaata atgaccggaa cttcatattt
      241 ttctgcctgt gcgcgcattt ttgataccag taaagaggta gacatgcccg cagaacaaaa
      301 cagataaatg tgtttctttt ccataaaact gccctcatcg acgattatct gtcagccaga
      361 cactccgcaa gccttaacct gcttccatgc tctgggtaac ttgcgaaacc aacatgactt
      421 tttttgtggc tgaaaggagt atacggtata gacctgagca atagtatttc tttgacctta
      481 taaattgtct ctccttgacc tgcggttatg accctcttca catttcgggc aaataattcg
      541 cgaagataat taagaactca ggccaaaaaa accggcgcaa tggccggttt ccgttgttac
      601 tcaagctttc aaacgaagtg attacttcgc agcctgtgga tcagcgtcgt attcagcaca
      661 ggtctgataa ccggagttga tgacatgtcc ggtatcatct aacgcgacaa agtaggtttc
      721 tgctttacca tcacgttgac ccaggatgta ggtctggcaa gtaccacgag catggatcat
      781 gctcacttca gacgaaggtt tacccgcaat ttgtgcaacc tgcgcccggc tcatgccttt
      841 tttgacgtct ttcaccacag gctgtacaaa ctggtctttg gtacgatcat aagccgtaca
      901 acccgccagc atggttaata ccgccgctgc actcagaatt cctgccatat tcttgttcat
      961 attccgtcct cttgtttatc agcgtgttag ataagcctgg aatacattgg gcgctttttc
     1021 aagcccgtga acgaaacggc tccgctttca gaggattcct gtatgacgtt ttatccacca
     1081 ttcaacccgc tgtcgcttgt cgtttcagta gcgacgggtt agctttaagg aagttttgtc
     1141 ttttctgtct ggaggggttc aatgacattg caacaacaaa taataaaggc gctgggcgca
     1201 aaaccgcaga ttaatgctga agaggaaatt cgtcgtagtg tcgattttct gaaaagctac
     1261 ctgcaaactt atccgttcat taaatcactg gtgctcggga tcagcggcgg tcaggactcc
     1321 acgcttgccg gtaagctgtg ccagatggcg attaatgagc tgcgccagga gaccggcaac
     1381 gaatcactgc aatttattgc cgtacgcctg ccctatggtg ttcaggccga cgaacaagat
     1441 tgccaggatg ccattgcctt tattcaaccg gatcgcgtat taaccgttaa tatcaagggc
     1501 gcggtattgg ccagcgaaca ggcgctgcgc gaagcgggca ttgaactgag cgattttgtc
     1561 cgtggcaatg aaaaagcgcg tgagcggatg aaagcacaat atagcattgc gggtatgacc
     1621 agcggtgtcg tggtgggcac cgatcatgca gcagaagcta ttaccggatt cttcactaaa
     1681 tatggtgacg gcggtacgga cattaatccg ctgtatcgtc tcaacaaacg tcagggtaaa
     1741 cagttactgg cggcattagc ttgcccggaa cacctttata agaaagcgcc aacggccgat
     1801 ctggaagatg atcgcccttc tctgccagat gaagtggcac tcggcgtgac ctatgacaat
     1861 atcgacgact atctggaagg gaaaaacgta cctcaacagg tcgccagaac aatagagaac
     1921 tggtatctga aaaccgaaca taaacgccgt ccgccaatta ccgttttcga tgatttctgg
     1981 aaaaagtaat aatttgcaca tattggattg tgcgaaaaag agtaatttgt tcacgccgga
     2041 tgcggcgtga acgccttatt cgacctataa aactatgcaa attcaataca ttgcaggagt
     2101 cgaataggcc tgacaggcgt agcacgtcag acggtgtaac ctttgtcatc gacccgcctc
     2161 ttttttaatc gcttcccgcc tgttacactg gatagataac cagcattcgg agtcaacagt
     2221 ggtacggcgt ttaacttctc cgcggctcga atttgaagct gcggcaattt atgaatatcc
     2281 cgaacattta cgttcattcc ttaatgactt acccacccga ccaggggtgt atctgtttca
     2341 tggtgaaagt gacaccatgc tgctctatat cggcaaaagc gttaacatcc gcagccgcgt
     2401 cctttctcat ttacgtaccc cggatgaagc cgccatgcta cggcaatccc gacggatcag
     2461 ctggatatgt accgccggtg aaatcggcgc tctgctcctt gaagcgcgat taatcaaaga
     2521 acaacagccg ctgtttaata aacggttgcg ccgcaatcgt cagctctgtg ccctgcaatt
     2581 aaatgaaaag cgcgtcgatg tggtgtatgc caaagaggtg gatttttcac gagcccccaa
     2641 cctgtttggc ctgtttgcca ataggcgcgc agctttgcaa gcattgcaga ccatcgctga
     2701 tgaacaaaaa ctttgttatg gcctgctggg actggaaccg ttaagtcgcg gtcgtgcatg
     2761 ttttcgttca gcgctaaaac gttgcgccgg agcatgctgc ggtaaagaga gccatgagga
     2821 acatgcgcta cgcttgcgcc agtctctgga gcgtttgcgg gtggtgtgtt ggccttggca
     2881 aggggcggtg gcgctgaaag aacagcaccc ggaaatgact caatatcata ttattcaaaa
     2941 ctggctgtgg ctgggggcgg ttaattcgct ggaagacgcg acaacgttaa ttcggacacc
     3001 cgccgggttt gatcacgacg gttataaaat tctttgtaag ccgctgcttt ccggtaacta
     3061 tgaaattact gaacttgatc cggcgaatga ccagcgagcc agttgatttc actgaacaac
     3121 agtttacctt gcggttgtaa aagccgcagg gtgtgcctgc catcgaacca gcacgcgccc
     3181 tggtcggtag tgagcaattt atcgcccagt tgccacgccc cgttaatcac aaacaccacc
     3241 ccgccgcgtg agccaaaggt agtaaacgta cgttcggcaa ttctgacttt cgccttacag
     3301 acatccaggc gtgtcatgat gttgaaatcc atcgacatct gcccttccgt cagtttcgct
     3361 tttaccacct ggtccgctgc aaaggcaaaa ggctgtaacg gttttaaggt atggttaaag
     3421 cggtctgcgc tttcaaggag catctcgccg ccttccagca acgtcactat cctttccatg
     3481 ccgggaaaca aagaaaactc cccattcgcc gcgatggatg caatgctggc acgccagtaa
     3541 aaatcacgtt ttgccggtgg gaaagtgcaa atttcgcgcg tttcaccggc agcatttcgc
     3601 cacagattca ccgacatttt acgcatatca aagtattcca tactcgctcc cggcccgttc
     3661 ttttcattgt tatcgttcag gtaaccgtgg atggttatcg gcaactcggg tgatttacgt
     3721 tagtggtgat caggcttttt ttacaactgt ttgagaagag aaaagaaaac cgccgatcct
     3781 gtccaccgca ttactgcaag gtagtggaca agaccggcgg tcttaagttt tttggctgaa
     3841 agattattca gcagttgcag gcattttacc ttttgccgct ggacgttctg tcagacgctt
     3901 ctcaaaatta gcattaaatt gctttttctg ttccggcgtc aggatgttgt aaattttgtt
     3961 ctgggtttcc atgtgcgcca gcatgttagc tttgcgctgt tcttccattt ttgcgatctg
     4021 cgcttcagct tttgctttat cgaaggtatc gctgacaatg atgtcatgca ttgcgcggcg
     4081 ttcttccagc ggcggacgtt tcatctggtc acgctggcct ttcatgattt cgcggatctg
     4141 ctgtttctgc gcgtcggtca ggttcaggtc tttgaacatc atgtcctgat gcggaccgaa
     4201 cttgcctttg tggtgcatca tcggcttcgc gtcagccggt gctgcggcat gggccaggtt
     4261 agccgcgcca agagccaggg tagaggcaac aaacagtgca gttaatttac gcatattcta
     4321 tatccttcct ttcagttatt tattacggct ttcttaagta gcgtgccgtg ttgacaagat
     4381 taactttact gactttagcg tcaattaatc agagcaacgg taaaacaatg aaagtgtaaa
     4441 aaacactttt gcgccaatta tggagaaaaa aagaaaattt gatggagagt gatgagagaa
     4501 tattacaaca caatgatttt gcagagatta tgaagaacta tactggatga ctggtgataa
     4561 ataaagcaaa taaccaggat taatctgtat taatttataa gaaagcaact taatacccgc
     4621 agaatgattt ctgcgggtaa gtattagctt attttttcga gcattaatcc cgcgcgtaat
     4681 cccaacgcta ccaacggatt agggaataac acatactcta catcatgggt tacggtaaaa
     4741 cgttcctctc cgtcctgcgc cagcaatgtt cctttctcaa acggcataaa attcagcgtg
     4801 tcacttgcca tatgcatttc gaaggacggc gagtggcgag taatttgcga aaccacccga
     4861 taacggagcg gcggtgttct cacgataccg acactctcac cagatagcag cgcagcaatt
     4921 gcgctggcag ttactgcaaa ctggcgaaga tcgttttgcc caaagggcaa cgctttgcca
     4981 agttccagcg tacaggccag cgcgccaaaa tgtctggcgc tgaaatgggt aaacgtacca
     5041 ccaggttcct gatggaacac cagcgcctcc agccccgccg cacccagcca cgtcagaaat
     5101 ttctcgtccc agggaatgtc gcgttgcggt aatacaccga actgcggatg caaggagcca
     5161 cgaattgcgg tatgtagatc aaggtgccag cgcacagatt ctttgccctg gtcataaaaa
     5221 tcttccaggc actgttccag ttcgcgcgcc cgacaggttt ctccgctttc agcaaatagc
     5281 tgccaacgac cgccaaacat tcgattcata tcgctatggc aataacgttt cccttgcttc
     5341 agcgcaggag gattcccgag gatcaccagc aaccgccaac gtaacgggat ctcgccgtga
     5401 gatatcgcgc caagcaacgc gtccagcatt tccacaggtg ccgtctcatt accgtggatc
     5461 cccgcagaaa tcaccagcgc gccttgcggt ggcgttaatg gcgtcagttc cagcacacca
     5521 tcgcccagcc agcgccagcg aacgccgttg atttctcgct cggtgataac cggtttttta
     5581 cccgttaagg tcagagcaag aaaattatcc attgccccct ccctcgcgct ggaacggata
     5641 aaccgaaccg agattcagta attggctcaa tacatccagt gcttcccgcc cttcgcgcag
     5701 caattgcggg tcggccagat cggcagcagt aagacgatcg cggtagtaac gatccaccca
     5761 gtcattgagc gcattaaaca aggtatcgtt catcatcacc gccggattca ccgcccggcg
     5821 ttcttcttct gtcaatacca cccgcaaccg caggcacgcc gggccgccgc cattcgccat
     5881 gctttcacgt aagtcaaaga cttttagttc gctaatcggg ttgtcagcgg caaggagttc
     5941 attgagataa ccccatactc cggcgtgttc ccgacactcc tgaggcagca ccaacatcat
     6001 ggaaccatca tcgcggctca acagttggct gttaaacaga taggtagaca ccgcatccga
     6061 cacggaaacc tgagttgccg gaacttctat cgccataaaa ccgttcaccc gcgcccgcag
     6121 gtttgccagt aactgtgact ggcgagcgaa cgcctgttgg tggcaaaaca gcacctggcg
     6181 gttacttacg gcaatcacgt cattatgaaa aacaccctgg tcgataacgt ccgggttttg
     6241 ctgggcgaaa atcacctgtt ggggattcac ctgattaagc cttgccaccg cctcgctggc
     6301 ttcgcgagtc tgtcgcgccg gataacggga aggccgggta tcattgcctt cttctcgccc
     6361 gtagacaaaa agttgcatac ccggctcacc gtaatgaccg ccgagacgat tgtggtttgc
     6421 cgccccctca tcaccgagca acgctacctg cggcaacgcc gaatggacgc taaatttctc
     6481 ttcgtcgtta aaaatagctt ttaacagcga ttcagtgacg ggcgcttcca gcgaacggtg
     6541 aaatttattg ttcaggttgg caacggtgag atgcactttg ccatccagcg tatcggcaga
     6601 tggcgcgatc gttgccgcat tggctaccca cattggcgaa gcggagctga cgctggaaag
     6661 ccagtgcggt gcctggcgtg caactttttc cagtacctgc tcatcgctac cactgaatcc
     6721 caactgacgc agcaccggaa taaacggacg ctcgtgcggc gggatcacgg cctgggggaa
     6781 tcctgcatcg gcaagggttt tcattttcag taagccctgt ttcgccgcca gtcgcgggtt
     6841 agacacctga aaacggtgac gggtagaggc ttcattacca aacgacaggc cagcgtaatg
     6901 atgcgtcagc cctaccagcc cgtcgaaatt aacttcccag gcgttcatcg caccacctca
     6961 tcggaaaaat ccagcccggg gttaagcgtt gcgggcaaag ttaacgagtc cgactccagg
     7021 ctcgccatag gccatgcgca gtaatctgcg gcataccagg cgctggggcg atggttaccg
     7081 gatgcaccaa tgccgccgaa tggcgcggta ctggcagcac cagtaagcgg tttgttccag
     7141 ttaacaatcc ccgcccgcgc ctccagcaac agttgatcga atttttcccg ttcgggggaa
     7201 accagaccgc aagagagtcc gaagcgagtg ttattcgcca ttagaatcgc ttcctcgaaa
     7261 gaatcataac gccagacgcg caataacggt ccgaacacct cttcatctgg tacgccagca
     7321 acgcctgtca tttcaatgat ccccggcgtc agcaacgatg tctctaattg taataagcgc
     7381 ggcgcaagca gggttcgtcc gcccatcgct tccagttgct gccaggcagt aaccacctgc
     7441 tgtgcggcct gttcagaaat cagcccgcca ataaacggct gcggttcgtc atcccagttg
     7501 cccggcgtta atcgctggct gacggcaacc agacgagcaa gaaacgcatc gccctgcgct
     7561 ccgcttttga gcaataaacg gcgggcgcag gtgcagcgtt gcccggctgt gacaaacgcc
     7621 gactgaatgg tcagatggac agccgcgtcg atatccgcca cctcatcgat aattagcgga
     7681 ttattaccgc ccatctcaag ggcgagaatt ttctccggct gaccggagag ctggcgatgc
     7741 agctggtagc cggtattggc gcgaccggta aacagcaaac cgtcgagatc ctccagcgca
     7801 ctcagcgcct gacccgtttc acgcccgccc tgcaccaggt tcagcacgcc cggcggcaag
     7861 ccagcctgct gccataaacg cattaccgct tcgccactcc acggtgtcag ttcgctgggt
     7921 ttaaagataa tggtgttacc tgccagcaat gccggaacga tatgtccgtt cggcaaatga
     7981 ccagggaaat tatacggccc aaacaccgcc agcacgccgt gcgggcgatg tcgcaggctc
     8041 gccgcaccgt ccggcatttc actacgctgc tcgccggtac gaacgtgata cgccttaatt
     8101 gatatcgcga ttttattgat catcgccgtc acttcggttg ccgcttccca gcgcagctta
     8161 cccgtttctc tggcaataat cgcggttaat tcggctttat tgctttccag caatccggca
     8221 aagcgttcga caacgacctg acgttcagcc aatgagagcc gcgcccagcg cggaaacgcc
     8281 gcacgggctg cccgacaagc ctgttcgacc tgagcggcat cggcatcatt gccttgccat
     8341 aacacctcgc ccgataccgg attacgcttc acacgcgatg cgccctggcc cgttatccag
     8401 tcaccgttaa tccataaagt catgctgttt tctccactgc gcacaggcgc accagacgaa
     8461 cgcgatcccc tgcgtggcat ttgagggcat ccagttgtgc ggcggttaaa atcaaacgct
     8521 cggttgccgg atcggtacgt gccagcacca cgcggaaatg gtgataattt tcattggcga
     8581 ccaggcaggc cggaaaatcg ccctgcgcag gctgcccttc tgccacttcc accagccgac
     8641 ttttacggat ggcgcgcacg cggtcgatgt cacactcaag cgttggcccg ccgtcgaaga
     8701 tgtcgatata gttgcggtaa cgaaaacctt ctttctccag caccgcgcgg gcaggcgcgg
     8761 tttgcggatg tacctgaccg atgacgtcct gggcttcctg ggataaaaag tgggtataga
     8821 tcggatgttt cggcatcagt tctgcaataa atgccttttg cccggtgccg cagagaaaat
     8881 cggcgcggct aaaatccatc gaaaagaagc gtttaccgag gctttgccag aacggtgaat
     8941 agccgtgttc gtcaatcacc ccgcgcattt cagcaaccac tttgtcatta aacttgtcgc
     9001 gaaaagccgc cataaacata aagcgcgatt tcgacagcaa atagccgttg ccctctttgc
     9061 gccagtccgg gtcgagaaac agcgtgcaca gctcgctgct gccggtgtga tcgttactaa
     9121 gaaacagcgt cggcaatgcg ttatagacat tcagctcttt tgaggcgtga accaacgtgc
     9181 cgacccgata gttgtgccag ggatcgttca gcccaaccgc cacctcaatg gcgcaaatcc
     9241 ccgccacggt gcctgtctcg ctatcttcca gcacgaacac atagccctgc tcgcttttgg
     9301 gcagttcgcc ttgccaggtt ttgattgccc tttcgatacg cgcggaaagc gtggcttcat
     9361 tagcgggaag cgacgtcagg ccgccgcccg ttttgctggc aagctgcatc agcgccgaga
     9421 catctgagcg ctcaacggga cggatgacca tcatgatgaa cctcggctaa caaaatgttc
     9481 gcaagcagct gcaaagcgat ccagtccggt cgtcacctct tcttcgctga cattgagcgc
     9541 aggcgcaaaa cgcaccacgt tgccacccgc aatcagtacc ataacgcctg ctttcgccgc
     9601 ctcctgagag atctgtttcg cttgtccggc gtaatcggca ttcagtacac agccaatcaa
     9661 caaacctaag ccgcgaactt cactgaacaa accgtagcgg tgattaatgg tattaagacg
     9721 ctcaacaaac cagtcgtgac gctgtttaac gccattaagc atctctggtg tgttgatgag
     9781 ctccagcact ttgcccgcca ccgccgaggc cagcggattg ccgccatagg tggtgccatg
     9841 agtgccaacg gtcatcacgc tggcgcactc ttcggttgtt aacaacgcac cgaccgggaa
     9901 atcgccgccc agcgctttgg cggtagttaa cagatcaggt gtcacgccgt agtgcatata
     9961 ggcatacagt tccccggtgc gcccgacgcc ggtttgtact tcatcaaaaa tcaacagcgc
    10021 attgtggcgg tcacacaatt cacgcagacc ttgtaaaaac gcgttgctgg ctggcaccac
    10081 accgccttcc ccctggatgg gttcgacaat caccgcacag gtggcgtcgt caatcagcgc
    10141 gctggcagag ttaatatcgt tatatgcagc atgacggata tctggtggca gtggcgcaaa
    10201 atcctgtgaa taggctggct gcccacccgc actgacagta aacagcgtgc gaccatgaaa
    10261 cgcattttta aacgccatga taccgctctt atggctgccg tagcggtcgt gagcgaattt
    10321 acgcgccagt tttagcgccg cttcgttggc ttccgcaccg gagttacaaa agaagacgcg
    10381 atcggcaaac gtggcgtcga tcaatttttt cgccagtcgc agtaccggct cgttggtgta
    10441 accgttgccg gtatgccaga acttactcgc ctgttcgttc agcgcttcac gcagttccgg
    10501 atgcgcatgg cccagcgcgt tcaccgcaat gccacccgcg aagtcgatat actctttccc
    10561 ctgctgatcc cacaagcgcg aaccttcgcc acgtaccggt ataaagggtg ccggagcgta
    10621 aacaggtatc atccattcat caaagttttc acgcgtaatt ggctgagaca tagcgacctc
    10681 tacagtaaat aattcgttat ttatatgtta acaataagta atgtttgcgc tgtaaatgta
    10741 gattgcaggg ttcgtgccag ccagtgataa aagtgcataa acggcggagg ctaactggaa
    10801 atcaaggagt tataaccaaa ccatatgcat ttaaagtgca tataaagtga atacgtttgc
    10861 gatgtgggtg aataaaaaga ataaaaaacg caatgttatg cagaagtaaa atataattct
    10921 ggaattgtga tcattgacga aatttactgg aaattactgc gccattctga cgcagcgcgc
    10981 accaaaagcg ggcatttttt gcgccatcgt tgacatcatt aacaaccatc gatcaaatca
    11041 cttaacaaca ggcggtaagc aacgcgaaat tctgctacca tccacgcact ctttatctga
    11101 ataaatggca gcgactatga aatttgtctc ttttaatatc aacggcctgc gcgccagacc
    11161 tcaccagctt gaagccatcg tcgaaaagca ccaaccggat gtgattggcc tgcaggagac
    11221 aaaagttcat gacgatatgt ttccgctcga agaggtggcg aagctcggct acaacgtgtt
    11281 ttatcacggg cagaaaggcc attatggcgt ggcgctgctg accaaagaga cgccgattgc
    11341 cgtgcgtcgc ggctttcccg gtgacgacga agaggcgcag cggcggatta ttatggcgga
    11401 aatcccctca ctgctgggta atgtcaccgt gatcaacggt tacttcccgc agggtgaaag
    11461 ccgcgaccat ccgataaaat tcccggcaaa agcgcagttt tatcagaatc tgcaaaacta
    11521 cctggaaacc gaactcaaac gtgataatcc ggtactgatt atgggcgata tgaatatcag
    11581 ccctacagat ctggatatcg gcattggcga agaaaaccgt aagcgctggc tgcgtaccgg
    11641 taaatgctct ttcctgccgg aagagcgcga atggatggac aggctgatga gctgggggtt
    11701 ggtcgatacc ttccgccatg ctaatccgca aacagcagat cgtttctcat ggtttgatta
    11761 ccgctcaaaa ggttttgacg ataaccgtgg tctgcgcatc gacctgctgc tcgccagcca
    11821 accgctggca gaatgttgcg tagaaaccgg catcgactat gaaattcgca gcatggaaaa
    11881 accgtccgat cacgcccccg tctgggcgac cttccgccgc taatttagca gctctcctgg
    11941 ctcaaactgg gtcaggagaa ttaaccttga gaaaaatcaa caaactgtca gtaatgattt
    12001 gttgcctgcc gtcctttgtt ataccgtcac tgcgttttta gttgtctgac cacttctcta
    12061 ttatcaagtt tgatatagga aactccacga tgaatgctga gcgtaaattt cttttcgcct
    12121 gtcttatttt tgcgctggtc atttacgcta tccacgcttt cggtttattc gatctgctca
    12181 ccgatttacc ccacttacag acactcattc gccagagcgg attgttcggt tatagcctct
    12241 atattctgtt attcatcatt gccaccctct ttctgttacc aggaagcata ctggtgatcg
    12301 ctggaggaat agtttttggc ccgctcttag ggacactact ctcattaatt gccgccacgc
    12361 tggcctcctc gtgctcattc ctgctggcgc ggtggctggg acgtgattta ctgctgaaat
    12421 acgtgggcca tagccatacc ttccaggcca ttgaaaaagg cattgcgcgt aacggtattg
    12481 attttcttat tctgacccgc ttaatcccgt tgtttcctta caatattcaa aattacgctt
    12541 acggattaac caccatcgcc ttctggcctt atacccttat ttcggcactc acgaccctgc
    12601 ccggtattgt tatttatacc gtgatggcaa gcgatctcgc caatgaaggc attacgct
//
LOCUS       DADLFA010000121        12565 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000121 DADLFA010000000
VERSION     DADLFA010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12565
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.121"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            206..1303
                     /gene="mltA"
                     /locus_tag="JQH65_002830"
     CDS             206..1303
                     /gene="mltA"
                     /locus_tag="JQH65_002830"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HAY6140194.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKSTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1380..2186
                     /gene="tcdA"
                     /locus_tag="JQH65_002831"
     CDS             1380..2186
                     /gene="tcdA"
                     /locus_tag="JQH65_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HAY6140195.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSIGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2237..2680)
                     /gene="csdE"
                     /locus_tag="JQH65_002832"
     CDS             complement(2237..2680)
                     /gene="csdE"
                     /locus_tag="JQH65_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HAY6140196.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVLGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRGQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(2680..3885)
                     /gene="csdA"
                     /locus_tag="JQH65_002833"
     CDS             complement(2680..3885)
                     /gene="csdA"
                     /locus_tag="JQH65_002833"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HAY6140197.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALSQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAENWSHSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4077..4304
                     /locus_tag="JQH65_002834"
     CDS             4077..4304
                     /locus_tag="JQH65_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HAY6140198.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            4655..5572
                     /locus_tag="JQH65_002835"
     CDS             4655..5572
                     /locus_tag="JQH65_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HAY6140199.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5591..5986
                     /locus_tag="JQH65_002836"
     CDS             5591..5986
                     /locus_tag="JQH65_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HAY6140200.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            5979..7079
                     /gene="rlmM"
                     /locus_tag="JQH65_002837"
     CDS             5979..7079
                     /gene="rlmM"
                     /locus_tag="JQH65_002837"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HAY6140201.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENSGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7123..7854)
                     /gene="fucR"
                     /locus_tag="JQH65_002838"
     CDS             complement(7123..7854)
                     /gene="fucR"
                     /locus_tag="JQH65_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HAY6140202.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICYELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(7912..8334)
                     /gene="fucU"
                     /locus_tag="JQH65_002839"
     CDS             complement(7912..8334)
                     /gene="fucU"
                     /locus_tag="JQH65_002839"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HAY6140203.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8336..9754)
                     /gene="fucK"
                     /locus_tag="JQH65_002840"
     CDS             complement(8336..9754)
                     /gene="fucK"
                     /locus_tag="JQH65_002840"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HAY6140204.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERTHAWFFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     SADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(9751..11496)
                     /gene="fucI"
                     /locus_tag="JQH65_002841"
     CDS             complement(9751..11496)
                     /gene="fucI"
                     /locus_tag="JQH65_002841"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HAY6140205.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEVLQERL"
     gene            complement(11529..>12565)
                     /gene="fucP"
                     /locus_tag="JQH65_002842"
     CDS             complement(11529..>12565)
                     /gene="fucP"
                     /locus_tag="JQH65_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HAY6140206.1"
                     /translation="ITGLFLYALGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAA
                     NPFVTVLGPESSGHFRLNLAQTFNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQ
                     LSAYKHSLVLSVQTPYMIIVAIVLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRL
                     ARIRHWRWAVLAQFCYVGAQTACWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGR
                     FTGTWLISRFAPHKVLAAYALIAMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFS
                     LGIKNLGQDTKYGSSFIVMTIIGGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIF
                     ARFRSQTATN"
BASE COUNT         3012 a         3139 c         3327 g         3087 t
ORIGIN      
        1 gtggatgcgc actatactcc gctcgcgttt tcatgcaacc ttttttttac cccgatcacg
       61 gatttagcat gattttgaac aaaaacaaac cactaaagcc tattttttgt gcaaaatttg
      121 cgtcagggag aggctgctca tttcattaaa cgcggcggtt tgttatcttc gttgcgcctt
      181 attttttaac ctgaagaaga gaacaatgaa aggacgttgg gtaaagtacc ttcttatggg
      241 cacggttgtg gcaatgcttg ccgcctgctc ttccaaatca accgatcgcg gacagcaata
      301 taaagacggg aaatttaccc agcctttctc tctggtgaac cagccagatg ccgttggcgc
      361 gccgattaac gccggtgatt ttgccgagca aattaaccat atccgtaatt cgtcaccgcg
      421 tctgtatggc aaccagagta atgtttataa cgcggtgcaa gagtggctgc gcgcaggcgg
      481 tgatacccgc aatatgcgcc agttcggcat tgatgcctgg cagatggaag gtgccgacaa
      541 ctatggtaac gtgcagttta caggctatta cacgccggta attcaggcgc gccatacccg
      601 ccagggcgag ttccagtatc ctatttaccg tatgccgcca aaacgtggtc gtctgccgtc
      661 tcgtgcggag atctacgcgg gggcattggg cgataaatac atcctcgctt acagcaactc
      721 tctgatggat aacttcatta tggatgtgca gggtagtggg tatatcgact ttggtgatgg
      781 cagtccgctt aactttttca gctatgcagg gaaaaacggt catgcctatc gcagcattgg
      841 taaggtgctg atcgaccgtg gcgaagtgaa aaaagaagat atgtcgatgc aggcgattcg
      901 tcattggggc gaaacacaca gtgaagccga ggttcgcgag ctgctggaac agaacccgtc
      961 tttcgtcttc tttaaaccgc aatcttttgc accggtgaaa ggggcaagtg cggtgccgct
     1021 ggttggccgt gcttctgttg cctccgatcg ttcaattatt ccgccaggta ctaccttgct
     1081 ggcagaagtg ccgctgctgg ataataacgg caaatttaac ggtcagtacg aattgcgtct
     1141 gatggtggcg ctggatgtcg gtggcgcaat caaaggccaa cacttcgata tctatcaagg
     1201 gatcgggccg gaagccggac atcgcgcagg ttggtacaac cactatggac gtgtctgggt
     1261 gctgaaaacc gccccgggcg caggtaacgt ctttagcggc tgatgtgtta ttctgtatac
     1321 cgtttttacg gatacgacag ggtgagggat aacctcaccc tttttaattc tgaggtgcta
     1381 tgtctgtggt aattagtgat gcatggcgtc agcgttttgg tggcacagcg cgtctgtatg
     1441 gtgaaaaagc gttgcaactg tttgctgacg cgcatatttg tgtggttggt atcggtggtg
     1501 tcggttcctg ggcggcggaa gcgctggcgc gcacggggat tggcgcaatc acgcttatcg
     1561 atatggatga tgtgtgcgtc accaatacca atcggcaaat tcacgctctg cgcgataacg
     1621 tcgggctggc aaaagcggaa gtcatggcgg agcgcattcg ccagattaac ccggagtgcc
     1681 gtgtaacggt ggtggatgat ttcgtgacgc cggataacgt agcgcaatat atgagtgccg
     1741 gttattcgta cgtgattgat gccattgata gtgtacgacc caaagcggcg ctgattgctt
     1801 attgtcggcg caataaaatc ccgctggtca cgaccggtgg cgcgggtggg cagattgacc
     1861 cgacgcagat tcaggttacc gatctggcga aaacgattca ggacccgctg gcggcgaagt
     1921 tgcgcgagcg cctgaaaagc gattttggcg tagtgaaaaa cagtataggt aagctcggcg
     1981 tggattgcgt gttttctact gaagcgctgg tgtacccgca gtcagacggt acggtgtgtg
     2041 cgatgaaagc cacggcagaa gggccgaagc ggatggattg tgcatctgga tttggcgcgg
     2101 caacgatggt gaccgccacc tttgggtttg ttgcggtttc tcatgcgctg aagaagatga
     2161 tggcgaaagc agcgcgtcag ggttaaacaa tcaggtcgga tgcgacgcga acgtcttatc
     2221 cgacctggtt ctgtgcttaa acctgcttcg ccgcagcgat aattgcctcg cttaacgcat
     2281 tcaacccctg gctgcgtgag gcgctaagct gcccacgcaa tcccagctca tcaaacaatg
     2341 ccagcggcga ctgtgcctgt aattcggcgg cggttttccc ctcaacggca gtcaacaaca
     2401 ccgccaacag gccgagcaca atgcgccctt cgctgtcgcc aaagaagtgc attttaccgt
     2461 tttcagccac ggtatatccc agccagacgc ggttttcgca tccggcaatc tctttagcct
     2521 gcgcttttaa ctcgtctggc aaagccggaa gctgtttccc caacatgatc aactggcgat
     2581 atttatcttc ccattgcgtc aacggtgcga aggtattgcg taacgtttct gcggttacgg
     2641 ttgtgccgaa cggatgtccg gcgaattgcg ggtttgtcat taatccacca ataattccag
     2701 cgcgcggtca acggcattca ccagcgcatc cacatcactc tttgtattat atggcgcaaa
     2761 agaggcgcgc agtgtgccgg ttacgcctaa ttctgccagt agcggctgag cacaatgttg
     2821 cccggcccgc aaggcaatac cgtactccgc cagtagcgtc accatatcgc tgtgatgaac
     2881 gccagcaaaa tcaaaggcca gcaggctgga atcctggcag cggaatgaac gaaagcctgg
     2941 acgtttcgcc agcgcatctt ccgccagcgt tgctaagcta tggctccagt tttcggcctg
     3001 gttgatatcg taatctgcca gccattccag cgccgcgctt aatcctatga caccagccac
     3061 atttggcgtt cctgcttcca gtttccacgg cgcagattga gtcgtgaagc cgtcaaaact
     3121 cacttcgtga atcattttgc cgccacccag ccagggcgac atcgcttcca gcagttctga
     3181 tttgccatac agcacgccga tacccgtcgg gccatacagt ttgtgaccag agaaagcata
     3241 gaaatcgata tccagttgct gaacatccgc ggggaaatgc accgccccct gagcaccatc
     3301 aaccatcacc accatccctg ctgaatgagc aaaggtaatc gctcgcgcca gatccgggca
     3361 accgccagta acgttcgaca tctgactcaa cgccagaatc cgactacggg gagtaatcag
     3421 ttctggcaac aaatcgacat ccggcagtcg ctgcgcatta agcggcaatt tcaccacttt
     3481 ggctccagtt tgttgggcga ccatcagcca ggggacgagg ttggcgtggt gttctgccac
     3541 gctgacaata atctcatcgc ccggttgcag acgcggacgc gcatagcatt gtgccaccat
     3601 gttgatggat tcagtggtgc cgcgcgtcca gacgatagtt ttatcatccg gtgcattcag
     3661 taattgcgcc actttctctc gtgcagcttc ataacgcgcg gtcaggcgtt gggcttcggc
     3721 aaactggctg cgatggacgt ttccggcgct caggctgtaa aactgtcgag tggcttcaac
     3781 cacggcttca ggtttaagcg cggtcgcggc gctgtcgaga tagacgcccg catcctgtag
     3841 tgcgggaaac tgggcgcgaa actgcgcggg attaaaaacg ttcatggtac tcctcggctt
     3901 gatgcaccga tcgtgacgca aaattcactt tgatacaagg agagtgctaa ccatcagaat
     3961 tatctggata tcctggcgaa gccgcgtaag taacatatgc tgaatagtgt taggcgttaa
     4021 ttttagcctg ttaatcaata ctattaagat ttattaatcg ctttaaggag aagatgatga
     4081 aaaagactgc cgcaattatt tctgcctgta tgctgacttt tgccctgagc gcctgttccg
     4141 gttcgaacta tgtgatgcac accaatgacg gacgtaccat cgtctctgac ggcaaaccac
     4201 agactgataa cgataccggt atgatttcgt ataaagacgc taatggcaac aaacagcaga
     4261 tcaaccgtac tgacgtgaaa gagatggtcg aactggatca gtaattcgcg atcgcaaggt
     4321 aaaaaaaagc accgcaatta ggcggtgcta cattaatcac tatggacaga cagggtaaat
     4381 gtacaggaag tgaaaaaagg tagctttgct accatggtct gaatcgcaga ccaattgcaa
     4441 acacaacaac acaacatcac aaccgtaagc caaaagttca ccagaacacg cattccgata
     4501 aaacttttcg ttccggctca ggaagtgccg ccactatagg tatttgctgg tagaagctca
     4561 acggacaatt tataatggct cagattaaaa aaactaatag gttacatagt gtgatctaat
     4621 tgttaaattc atttaacatc aaagtttaat agccatgtct aaacgattac caccgctaaa
     4681 tgccttacga gtttttgatg ccgcagcacg ccatttaagt ttcactcgcg cagcagaaga
     4741 gctttttgtg acccaggccg cagtaagtca tcaaatcaag tctcttgagg attttttggg
     4801 gctaaaactg ttccgccgcc gtaatcgttc actcctgctg accgaggaag ggcaaagcta
     4861 tttcctcgat atcaaagaga tattttcgca attaaccgaa gcgacgcgta aactccaggc
     4921 ccgtagcgcc aagggggcgt tgacggtcag tttactcccc agtttcgcca ttcattggtt
     4981 ggttccgcga ctttccagct ttaattcagc ttatccggga attgacgttc gaatccaggc
     5041 ggttgatcgt caggaagata agctggcgga tgatgttgat gtggcgatat tttatggtcg
     5101 gggcaactgg ccggggctac gggtggaaaa actgtacgcc gaatatttat tgccggtgtg
     5161 ttcgccgcta ctgctgactg gcgaaaaacc cttgaagaca ccggaagatc tggctaaaca
     5221 tacgttatta catgatgctt cgcgccgtga ctggcagaca tatacccgac agttggggtt
     5281 aaatcatatc aacgttcagc aagggccaat ttttagccat agcgccatgg tgctgcaagc
     5341 ggctatccac gggcagggag tggcgctggc aaataacgtg atggcgcaat ctgaaatcga
     5401 ggccggacgt cttgtttgcc cgtttaatga tgttctggtc agtaaaaacg ctttttatct
     5461 ggtttgtcat gacagccagg cagaactggg taaaatagcc gcctttcgcc aatggatcct
     5521 ggcgaaagcc gctgctgaac aagaaaaatt ccgctttcgt tatgaacaat aatttacgta
     5581 gggtacgacc atgaccagcc gttttatgct gattttcgcc gccattagcg gcttcatttt
     5641 tgtggctctg ggcgcttttg gcgcgcatgt gttaagtaaa accatggggg ccgttgagat
     5701 gggctggatc cagaccggcc tcgaatacca ggcgtttcat acgctggcga tcttaggtct
     5761 ggcggtggca atgcagcgtc gcatcagtat ctggttttac tggagtagcg ttttcctcgc
     5821 gttaggcacg gtgttgttca gcggcagcct ttattgcctg gcgctgtccc atctgcgttt
     5881 gtgggcgttt gtcactccgg ttggcggcgt gagcttcctc gcgggctggg cgttaatgtt
     5941 agttggtgct atccgtttaa agcgcaaggg cgtaagtcat gaataaggtt gtattgctgt
     6001 gccgtccggg ctttgaaaaa gagtgcgccg cagaaattac cgataaagcc ggccagcggg
     6061 aaattttcgg ttttgcccgc gtgaaagaga attcgggtta tgtcatttat gaatgttatc
     6121 aacctgatga tggcgataag ttaatccgtg agctgccgtt cagttcatta atttttgccc
     6181 gccagtggtt tgtggtgggg gaactcctgc agcatttgcc gccagaagat cgtattaccc
     6241 ccattgtcgg catgttacag ggcgtagtag agaagggcgg tgaactgcgt gttgaagttg
     6301 ccgataccaa cgaaagcaaa gagttgctga aattctgccg taaatttact gttccgctac
     6361 gcgctgcctt gcgcgatgcc ggggtgctgg cgaactatga aacgccgaag cgtccggttg
     6421 tgcatgtatt ctttattgca ccaggctgct gctataccgg ttactcatac agcaacaata
     6481 attcgccgtt ctatatgggc attccgcgcc tgaaatttcc ggcagaggcg ccgagtcgtt
     6541 ccacgctcaa actggaagag gcatttcatg tgtttattcc tgcggatgag tgggatgaac
     6601 gcctggcgaa cgggatgtgg gcggtggatt taggcgcttg ccctggcggc tggacctacc
     6661 aactggtgaa gcgcaacatg tgggtttatt ccgtcgacaa cggcccgatg gcgcaaagtc
     6721 tgatggatac cgggcaggtg acgtggctgc gggaagacgg tttcaaattc cgtccgacac
     6781 gcagcaatat ctcctggatg gtatgcgata tggttgaaaa accggctaaa gttgcggcat
     6841 tgatggcgca gtggctggtt aatggctggt gccgtgaaac cattttcaat ctcaaactgc
     6901 cgatgaaaaa acgctacgaa gaagtatcac acaatctggc gtatattcag gcacagcttg
     6961 atgaacatgg cataaatgct cagattcagg cgcggcagtt gtatcacgat cgcgaagaag
     7021 tgacggtgca cgtccgccgc atctgggctg cggtgggtgg tcgtcgcgac gagcgataac
     7081 aggcaaggcc ggggcgtcgt cttcggcctg ctaccctttc cgtcaggctg ttaccaaaga
     7141 agttgcaacc tggcgctcat cagaaataat gtgcgttacc tcatccagat gcccgatgcg
     7201 ggcttcccct gaacgattaa atttactttt atcaatcaat aacaacgatt gcgcggcacg
     7261 ttttaatagc atcgatttgt aatcagcgtt gatcgcattg gagtcccaca gtgcgccgct
     7321 gctatcgatc ccttcacaag aaaaaataaa cagatcgatt tccagcgatt taagttggga
     7381 aatcagcgag ggattgacgt aacagccata tttgcgctca agtgtgccgc cggaactgat
     7441 cagttgaatg cgttcgcgtt taccgagttc atagcaaatc ggatggctat tggtgaagac
     7501 ctgaatgttg atgtcaggca actggcgtgc cagataccag caagttgaac tggcgtctaa
     7561 ggctatcacc atcccttctt caatccacgc gagcgcctcg cgcgcgatat ctgctttatg
     7621 cgcataatgg cttttcagcc tgatgtgaaa gggatcgcca ctgtcttgat tttgacggtg
     7681 gatatattta gcgcgtccat gattgcgcag aattttaccc tgcgtctgta attcattgag
     7741 atcgcgacga atggtttctt tactgacctt tagctgttca gagagagctt ccgtggtcag
     7801 gctggtatgg ttcagcagca ggtcgactat cgcttgctgg cgtgccgctt tcatattttt
     7861 caccccatca ttcgataacc gcccgcgtca tgcgggcgta ccggtatgag attacggtgt
     7921 tacccctttt tttaaaagaa tattcccgta cttcgctcgt tcgcctgtga taacgatcgc
     7981 aaaggctttt tgcgcccgtt cataaaacgc aaaacgattg atgcggatga tgtcaggaca
     8041 cggggcttgt agtgaaagcg cattacggta acgtcgttct acttcaggat cgagagtgtc
     8101 accttctacc gccgccatca tcaccagcgg cggtgcataa ctgtccagtt caaataacgg
     8161 gataatcgcc tggagcaagt cgctcaccaa caggccatca gcgcggatca cctgcggtcc
     8221 catcgaatgg gcgggaaagt gagcatcgga aaaaataatt tcatctccat gtcccatctc
     8281 tgccagcact ttcaatagtt cgggagaaat taacggcgaa attgttttca gcatttcaca
     8341 cttcctctat aaattcaggt tcagtttgcg ggtagaaata acggtactga taatgaatct
     8401 gtgcgcgggc ttcttcaggg ctgttaaatt cccctacgcc ataccaaccg aacagcgcag
     8461 ctcctgcgac ggtcgtttcg gcgtcgtcga gaacttttac cggaatatca agcatattgg
     8521 ctttaatctg attccacaat gtgttgcgac ttcctccacc gactaacaat aattcagagg
     8581 ccttaaagtg cccgattttt tccagcatct gtagattgcg ctgtaattgc gcagttaacc
     8641 cttccagcgc cgcgcgatag aaatgccccc gcgtggtatt aagcgtcact ccttgccagc
     8701 cagcgttctg acacgacaat aaatcacact gcatttttac gccatccgcg ctaggcgcga
     8761 taagacgagc ttcttcaatc aacatttgcc agggtgtttc agccgtccag aacagttttc
     8821 tcacccattc cagcacgccg gatgccagcc attgcatacc tgggttatac aaccctgcct
     8881 ggctatccag ttcgcaggtg gaaccggcgt actgacttaa cagcgaagta tcaacctggg
     8941 cgctacggac cattaaaatt tcccatgtac cggaagagag cacgggttca ttttggtcgg
     9001 caccagcgcc aaaaagggcg aactgggtat cgtgacctgc ggaaatcacc ggtatgccaa
     9061 cgggtaagcc gagcattgct gcggcgctgt tctgtagcgt accaatctgt tcacccgctt
     9121 ccaccagacg agggaagagt cggcgtggaa taccagtggc ttgtaaaatt tgcggactga
     9181 aatcgcgttg ctggatatcc agcatctggc tggttccggc catcgtgata tcagtagtga
     9241 attcgccggt taaacggtgg ttaatcagcg acgaaataaa gaaccaggcg tgcgtgcgtt
     9301 ccagcagttg tggatgattt tctttcaacc acaccaactt atataacgta ttgaaactaa
     9361 aggctccgac gccagaaata gcctgcaacc gctgtgcgga gatcaactgt tcaatattgt
     9421 ccataaccgc tgctgttcgc ggacatttcc agctaatgat cggatagagc agattgcctt
     9481 gcttatctac cagagcgcca tccacaccaa aggtggtgac ggcgataccg cggatgtggc
     9541 attcagtcag ttcactattg atttgccgac agcaatcagc aaagcgttgc aaaatggcgt
     9601 ctaaagacca ctggtgccag gtgttgtttt ccatcgcgat atcgctggca ttaggcgttg
     9661 aggcgcgggc aacaattttt ccctgccgat taaccgcgat ggccctgaca ttggtcgcgc
     9721 cacagtcgag taccaggata acttcttgtt tcataatcgc tcctgcaata cttcaatatc
     9781 catgccgtgc gcagcccagg cagaaggacg atacactttg gtctcttcaa cgttgtgcat
     9841 acataccggg atacgcagca tggaggcgag agtgataaag tcagcgccaa cgtggccgat
     9901 ggtcagaacc ccatggttag cgccccagtt cgccattacc gagtacacat ccgtaaacgg
     9961 ccctttaccg gtgaggcgcg gtgcaaacca ggtggttggc caggttgagt tggtgcgttt
    10021 gttgaggatg tcatgcacat ccttcggcaa ttccacgctc cagccttccg cgatttgcag
    10081 taccggtccc aggcctttga tgatgttgac acgagtcatg gtgaacggga cgccgccttc
    10141 ggtaaggaag cgggaagagt aaccgccgcc acggaagtat tcgtggatcg ccgggcacca
    10201 ttcggtagcg gcgaggcaag cgtcagcctc ttgctgagag atttcccagt gcggcttcat
    10261 cgtcgggtta ccttcgctgt cgcgttgttt acaggaaccg tccagcgcag cagaacctga
    10321 gttgatcaaa tggatgatgc cgtgttctgc cagtccatcg agtttatgcc ccgttacacg
    10381 ctcaattgct tctggtgacc agtaggtacg cacatcggca aatacctgag cggtgccggt
    10441 gagctggtga cccattagca ttgccacgcc gttaagactg tcgttttcgg tcgccacgac
    10501 aaagggttcg cgcacgccat tccagtcaaa tgaactgttg aggatcgctt cggcggtgtc
    10561 accattggga tattgatcgg tccagtgacg ttgcccctgg aagcccgcag cgatggcgtt
    10621 gtagccaagt gattcttcca cgcgaccaat atcggccagt ttgctgttgc cttgcatcat
    10681 gtcgcggata cacatcgcca tcagtaaact ttcgcgcaga actgcgcggc tttgctcggc
    10741 attgcgttga tactgtttgt tattttcatc ttcgccatag cggaagtttt tatcagccca
    10801 ggccagtgcc atttccagtt cggcttcgtc gtaaatcttc tgatcgatac ggcgacgcag
    10861 ttcggtcata tccaccgcct ggactttcat tcccagccag gattcaaaga agttgtgatc
    10921 aacgatggaa ccggcgatac ccatcgaaac gccgcccagc gacagatagc ttttaccttt
    10981 catgctggcg acggccaaac cggcgcgggc aaagcgcagc agtttttctt caacatcggc
    11041 aggaatcgat gtgtcatcgg catcctgaac gtcatgaccg taaatggaga atgccgggat
    11101 gcctttctgg ctgtgagctg ccagagccgc tgccaggtaa acagcgccgg gacgttcagt
    11161 gccgttaaag ccccaaatgg ctttcgggcg ggttggatcc atgtcgatgg tttcactgcc
    11221 atagcaccag caaggcgtta ccgtaatggt gaggcctaca ttctggctgc tgaatttttc
    11281 ttcgcaagca gcggcttcag ccatacccgc gatacaggta tcggaaatga cacactcgac
    11341 ggcagctccg caggcatggc gcagtttctc ggtcagcagt gcggccgtag ctttcgccat
    11401 attcattgtt tgttcttcaa gcgactcacg aacacccatg cgacgaccgt caataaccgg
    11461 gcggatacca attttcggta agctgatttt tttcattaca gattcctcac tttattcgga
    11521 aaatatgttc agttagttgc cgtttgagag cggaaacggg caaagataaa gatgaccgcg
    11581 aagcagagtg cggggatcag ttcagcagtg gggatgttgc ccgccgcgtc actgacaaaa
    11641 cccatgaccg gagtgacaat accgccgcca ataatggtca taacgatgaa ggacgaaccg
    11701 tatttggtgt cctggccgag attcttaatg cccagcgaga agattgttgg gtactgaatc
    11761 gacataaagg cgctgcataa agtcagggct attaagccca catgaccgcc agcgaaggct
    11821 gagatcaggc acagtgccat agcgattaat gcgtaggcgg ccaggacttt gtgtggtgcg
    11881 aagcgactga tgagccaggt accggtgaaa cgaccaataa agaagcacac catggttccg
    11941 gttaaatagt tagcggcaaa gcctgcagtc atacctggaa tttcttctac agcgtagcga
    12001 atcaaatagc tccagcaggc cgtttgtgcg ccaacgtagc agaattgcgc taatactgcc
    12061 cagcgccagt ggcgaatacg cgccaggcga gaaagcgatg cggagaacga tccttgtttg
    12121 gcgtcactgt gattatcact ctgcaatgcc gggaatttcg tcagcatgat cagcagggcg
    12181 accagtaaca cgatagccac gatgatcata taaggtgtct gtaccgataa taccaggctg
    12241 tgtttatacg cactcaattg ctctggagac attttatcga gaacgtcttg cgattgatgt
    12301 ggcacgttag acaaaataag actttgccca aagacaaccg cgataattgc gccaaacgag
    12361 ttaaatgttt gcgcaagatt taagcggaag tgaccactgc tttccggccc taataccgta
    12421 acaaaagggt ttgcggcagt ttccagacaa cctaatccgg ctgcaataat aaataggcca
    12481 actaaaaata aggtgtagtt cattatttct gcagcgggcc agaataatgc agcgcccagg
    12541 gcatataaaa ataacccagt aataa
//
LOCUS       DADLFA010000122        12536 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000122 DADLFA010000000
VERSION     DADLFA010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12536
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.122"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(93..1184)
                     /gene="ychF"
                     /locus_tag="JQH65_002843"
     CDS             complement(93..1184)
                     /gene="ychF"
                     /locus_tag="JQH65_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAY6140207.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1301..1885)
                     /gene="pth"
                     /locus_tag="JQH65_002844"
     CDS             complement(1301..1885)
                     /gene="pth"
                     /locus_tag="JQH65_002844"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAY6140208.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2163..2441
                     /gene="ychH"
                     /locus_tag="JQH65_002845"
     CDS             2163..2441
                     /gene="ychH"
                     /locus_tag="JQH65_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HAY6140209.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2496..4175)
                     /gene="dauA"
                     /locus_tag="JQH65_002846"
     CDS             complement(2496..4175)
                     /gene="dauA"
                     /locus_tag="JQH65_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HAY6140210.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVIIYPVSQQFGL
                     AGLLVAALLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPISGRLAFFPNRRAAMADL"
     gene            complement(4300..5247)
                     /locus_tag="JQH65_002847"
     CDS             complement(4300..5247)
                     /locus_tag="JQH65_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HAY6140211.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5398..6249)
                     /gene="ispE"
                     /locus_tag="JQH65_002848"
     CDS             complement(5398..6249)
                     /gene="ispE"
                     /locus_tag="JQH65_002848"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAY6140212.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6249..6872)
                     /gene="lolB"
                     /locus_tag="JQH65_002849"
     CDS             complement(6249..6872)
                     /gene="lolB"
                     /locus_tag="JQH65_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HAY6140213.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTHPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7086..8342
                     /gene="hemA"
                     /locus_tag="JQH65_002850"
     CDS             7086..8342
                     /gene="hemA"
                     /locus_tag="JQH65_002850"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HAY6140214.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8384..9466
                     /gene="prfA"
                     /locus_tag="JQH65_002851"
     CDS             8384..9466
                     /gene="prfA"
                     /locus_tag="JQH65_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAY6140215.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELRKAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9466..10299
                     /gene="prmC"
                     /locus_tag="JQH65_002852"
     CDS             9466..10299
                     /gene="prmC"
                     /locus_tag="JQH65_002852"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAY6140216.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGGREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10296..10688
                     /locus_tag="JQH65_002853"
     CDS             10296..10688
                     /locus_tag="JQH65_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HAY6140217.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            10692..11502
                     /locus_tag="JQH65_002854"
                     /pseudo
     CDS             10692..11502
                     /locus_tag="JQH65_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
     gene            11538..12392
                     /gene="kdsA"
                     /locus_tag="JQH65_002855"
     CDS             11538..12392
                     /gene="kdsA"
                     /locus_tag="JQH65_002855"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HAY6140218.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
BASE COUNT         3121 a         3181 c         3262 g         2972 t
ORIGIN      
        1 ggtgaaatga aaaatccacg caattgcgtg gattttatat acttttgcgc tcttcatgag
       61 atttagcgaa acctcatgag acaataaatt aattagacgt tgaaaaggaa gttcatcaca
      121 tcgccatctt tcacgatgta atctttacct tctgcacgca ttttgcctgc ttctttcgcg
      181 ccttgttcac ctttgtaagt gatgaaatct tcaaacgaga tggtttgtgc acggataaag
      241 cctttttcaa aatcagtatg gattttgccc gctgcctgcg gcgcggttgc tccaaccgga
      301 atggtccatg cacgcacttc tttcacccca gcggtgaagt aagtttgcag gttcagcagc
      361 ttataaccgg cacggatcac acggttcagg cccggctctt ccagcccaag ctcctgcata
      421 aactcgtcac gttcttcgtc gtccagttcg gcaatgtctg cttcaacagc agcacaaacc
      481 ggaaccacaa cagaaccttc tttcgccgcg atttcacgca cctggtcaag atatgggttg
      541 ttttcaaaac cgtcttcgtt gacgttggcg atgtacattg ttggtttcag cgtcaggaag
      601 ctcaggtaac gaatagccgc tttctcttca gcgcttaaat ccagcgcgcg cagcatacct
      661 gcattttcca actggggcag gcatttttcc aggaccgcca gctcagcttt cgcgtcttta
      721 tcgccacctt tggctttctt ctgtacgcga tgaatcgcac gttcgcaggt gtcgaggtct
      781 gccagcgcca gttcggtgtt gataacttca atatcgtcag ccgggttaac tttgccggaa
      841 acgtgaatga tgttgtcatt ttcaaagcag cgaacaacgt gaccgatcgc ttcggtttca
      901 cggatgttgg tcaggaactg gttacccaga ccttcgcctt tcgatgcgcc ttttaccaga
      961 ccggcgatat cgacaaattc catggtcgtg ggaagcgtac gctgcggttt tacgatttca
     1021 gccagttgat ccaggcgagg atcaggcatt ggtacgacgc ctgtgttcgg ctcaatggtg
     1081 cagaatggaa agttggccgc ttcaataccg gctttggtca gcgcgttgaa cagggtagat
     1141 ttcccgacgt tgggcaaacc gacgataccg catttgaatc ccatgattta aatcacctta
     1201 atatcttaat aatcaacctg ttattgctaa cagattgcag aaatggaaat aactttgcct
     1261 attatacacg gcactcggca aaaatgccgc agacaacgac ttattgcgct ttaaaggcgt
     1321 gcaatcggtt cgttgctttg gtcaagccat ctgtaaacca catttcagta caacgcgccg
     1381 cttcgtcaat ggcttcatca attaacttct gttcactaac aggcggtttg cctaacacaa
     1441 aaccgacaac tttattttta tcgcccggat gaccgattcc gatgcgtaaa cggtgaaagt
     1501 tagggttatt acccaattta ctgatgatgt ctttcagtcc attgtgacca ccatggccac
     1561 cgcccaattt aaatttggcg acgccaggag gcagatccag ttcgtcgtgg gccaccagaa
     1621 tttcgtccgg attaatgcgg aaaaaactgg ccatcgccgc aacggctttg ccgctgagat
     1681 tcataaatgt agtcgggact aacaggcgga catcttcgcc tccaagagtg actcgcgaag
     1741 tataaccaaa gaatttagcc tcttcgcgca gcggagcgcg caaacgctct gccagtaagt
     1801 caacgaacca ggcaccagca ttatgtcgcg ttgcggcgta ttcagcaccg gggttcgcca
     1861 ggccgacaat caatttaatc gtcacgtttt tttgtcctga gtgtgtacat aactggcgcg
     1921 tagtttactg gttgcgaccc cgcttgacaa aaaactgcgt atcaaatgca gataacgtaa
     1981 taattgcctg agtgggctat tagaaagtca aggtgttcag gcgtttattt gtaaagtttt
     2041 gttgaaataa gggttgtaat tgtgatcacg cccgcacata acccacaggg tgttgtctat
     2101 actttacaca taaggaagag gggtattccc tgttacaacc cagaaagttc cggaggtgac
     2161 atatgaaacg caaaaacgct tcgttactcg gtaacgtgct catggggttg ggtctggtgg
     2221 taatggtggt cggcgtgggg tattcaatcc tcaaccagtt accacagttt aatatgcccc
     2281 agtatttcgc acatggtgca gtgctaagta ttttcgtcgg tgccattctc tggctggcgg
     2341 gtgcccgtgt tggcgggcat gaacaggtgt gcgaccgtta ctggtgggtt cgccactatg
     2401 acaaacgttg ccgccgtagc gataatcgcc gtcatagcta acaaataatg cagtttgctg
     2461 accagtcagt ttgcgctgac tggtcaatct cgtacttata aatccgccat cgccgcgcga
     2521 cgattcggga agaacgcgag gcgtcccgag attggttgaa tgcctgcgcg agccatagtg
     2581 cgcagtggct ggaattccac gttgcacacg cgcagttcac atccttcggg cagacgcttc
     2641 acaaaacgct ggaacgcatc aagaccacca gcatcaagta ccggaacggc atcccacttc
     2701 agaatcacaa tccgtttgcc ttcaagacgt gactccaggt ccgtgaacaa gccttcagca
     2761 gcagcaaaaa acagcgggcc aataacgcgc agtaccagaa catcgtctgg aacatctacg
     2821 actaccggtg ccaggcgagt catacgtgcg atacgacgca taaacagcag cgatgccagc
     2881 acgatcccca cgctgatggc aataaccata tcaaacagca cggtcagcga catgcacagc
     2941 agcatgacaa tgatgtcatc tttcggtgca tgacgcagca agtcgaccac tttatgcgct
     3001 tcactcatgt tccacgccac catcaacagc agggctgcca tagcggaaag cggcagccag
     3061 gagagcagcg gtgccagtac cagcagggca agaataacca gaatagagtg gatcaccgct
     3121 gagatagggg aagttgcccc ggcacggacg ttagcggcag aacgcgcgat ggcagctgta
     3181 gcggtaatac caccaaagaa tggagcgata atattcccca gtccctgtcc aaccagttcg
     3241 ctgttcgcct tgtgtttcgt cccggtcata ccatccagta ccacggcgca gagcagagat
     3301 tcgattgcgc cgagcattgc cattgagaat gccgcaggca gcagtgtgcg aatagaatcc
     3361 caggttagcg tgaattctga attaggcaga tcccacggca gcaccagttg cggcagcagt
     3421 tgcggaatac cgttaccctg agaaccatcg gccaggacgt agtggaattg cgaaccgatg
     3481 gtagcaacat gtccgccgag caggttaaca atccccatca ccgcgcaacc agccagcaag
     3541 gccggaaggt gaccgggtaa acgaatgccc agacgcggcc aaaaaacaag aatacctagc
     3601 gtcacaatgc caatggcagc atcacccaca ttaatggtcg gcagcgccat aaataatgcg
     3661 ccgacttttt gtagataatg ttccgggaca tgggccattt gcagaccgag aaaatcttta
     3721 atctgcatgg taccgatggt gatcccgata cccgaggtga aacctaaggt gacggaaacc
     3781 ggaatatact caatcaggcg accaaagcgt gccagaccca tcagaatcaa aaagatcccc
     3841 gacagcaagg ccgcaaccag cagtcctgcc agtccaaact gttgcgacac gggatagata
     3901 attaccacaa atgccgcagt cggaccggaa acgctaaagc gtgacccacc cgtcagagca
     3961 atgacaatcc ccgcaacagc tgcggtatat aaaccgtact ggggtgccac accactacca
     4021 atagccaacg ccatcgccag cgggatagca ataatcccga cggttatccc ggcaatcagg
     4081 tcacgggtaa accgtgcggc agtatatttt tctttccagc aagcgtcgat cagagcgcgg
     4141 aaaggcatca catgtgagga aaatattttg ttcacaataa tgtttcatcc gtgagcgcat
     4201 catctgtcaa ctaaatggca ggtgaaggag gcataggtca tacaaatgga tattacagac
     4261 aaaaaaaccc gccgcagcgg gtctttgagc cgggttcgat tagtgttcga acatggcaga
     4321 gatcgattct tcgttgctga tacgacgaat cgcttcggcc agcatacctg acagggtcag
     4381 agtacgcacg ttcggcagtg atttgatttc atcgctcagc ggaatggtat cgcagacaac
     4441 gacttcatca attacagagt tacgcaggtt gttcgccgcg ttgccagaga agatcgggtg
     4501 agtcgcgtac gcaaatacac gtttagcacc acgttctttc agagcttcag cagctttaca
     4561 cagcgtaccg ccagtgtcga tcatatcatc gaccagtacg cagtcacgac ctgcaacgtc
     4621 accgatgata tgcatcacct gggaaacgtt cgcacgcgga cgacgtttgt cgatgattgc
     4681 catatcggta tcgttcagca gcttagcgat agcgcgggca cgcacaacgc cgccgatgtc
     4741 cggagaaacc acaattgggt tatccagatt cagctgcagc atgtcttcca gcaggatcgg
     4801 gctaccaaat acgttatcaa ccggaacgtc gaagaaaccc tgaatctgtt cagcgtgcag
     4861 atccactgtc agcacacggt caacaccgac gctggagagg aagtctgcaa ccactttcgc
     4921 agtgattggt acacgagcgg aacggacgcg acggtcctgg cgcgcatagc caaagtaggg
     4981 gataacagcg gtgatacgac ctgcggaagc acgacgcagg gcatcaacca taacgactaa
     5041 ttccatcagg ttgtcgttag taggggcaca agtggactgg atgatgaaaa tatcaccacc
     5101 gcgtacattt tcattaattt gtacgctgac ttcgccatcg ctaaagcgac ctacagcggc
     5161 gtcgccgagt gaagtgtaca ggcggttggc aatacgttgt gctagttccg gggtggcgtt
     5221 accagcaaaa agcttcatat caggcacgag aagaacctca ggcatgcgtc cattggtgga
     5281 aagaatctgc cgaaaactgt gcgggccagg catgatcctt tccaggcagt gtattaaaga
     5341 gcgcgatgca acgtctggaa caaggtgacg ttgtcaccga aactcagctt gcccggctta
     5401 aagcatggct ctgtgcaatg gggaaagatt aacgcctttc gccacaaagc cattgagcca
     5461 ttccggggct tgctctagca cctggcgggc ttcagactct gtatcaaatt cagcaaagac
     5521 acaggcccct gtcccagtca ggcgcgacgg ggcgtattct aacagccagg aaagcaccgc
     5581 atcaacctcg cgaaaacgtt ttcttgcgat aacctcgcaa tcattgctga attcacattt
     5641 tagcaacgtt tctattgacc tttttggcgt attgcgcggg agttcaggat ctttaaaaat
     5701 caccggagtc ggaatactta caccagggtg cgccaccaga taccacttct ctggcggatc
     5761 caccggcgtt agtatttcac caacgccttc ggcaaacgcg gcatgccccc gaacaaagac
     5821 aggaacatct gcgcccagcg tcagccccat ttccgccagc tcatccatgc ttagcccgca
     5881 ttgccagaga tgatttaatg ccaccaggac cgtcgcggca ttggatgaac caccgccgag
     5941 accgccgccc atcggcaaac gcttgtcaat gctgatattc gcaccgcttc ccgtcggaag
     6001 acgcccgctg tctgccgcag ttttcatcaa caatcgcgct gcgcgaacga tcaggttatc
     6061 ttcatgttcc acgccttcaa cgggcgttaa cagacgaata tccccatcgt cacgaagctc
     6121 aatgctgatg gtgtcgccgt aatcaagaaa ctgaaacagc gtttgcagcg tgtggtaacc
     6181 atccgcacgc tgaccggtaa tgtataaaaa cagattaagt tttgccggag agggccactg
     6241 tgtccgcatt atttcactat ccagttatcc atttttaact tgatgcgttg accaccgtcg
     6301 gtgagttcca tattggctgg catcgcagga tgcgttttgg tgtcataacc accataaaca
     6361 accttccagt ttttgccatt ctggctgtag gtaatttcgc tcaggcggta ctggtcgtcc
     6421 agtttgtagt cggttgcatc acccggtaaa cctaaaatcc actggcgcaa gctgttgagc
     6481 ggaattggca ttccggtcaa tttgccaatc atctcttcgg cgtcatcggc ggtataacgc
     6541 tgacctttat tgtcgactaa ctgcacgtta cccggttgag cattcagctc cagttccgtg
     6601 ctgcccaatg ggttagtgag cagcagacgg tagcgatcct ggccggtttg ctgccagaaa
     6661 aagcgggcgt acactttttg ttggtcagaa atataagcga acgcgccgcg agtctgatac
     6721 tgattaagat tgcgcacgtc ttgctgatgc tgacgccatt gtggcgaatc cgggcttttg
     6781 ccaggacctt tgggcgtggt aacggaacag gcagtgagca caagagcagc cagcggtagc
     6841 aggcggataa gacgaaaatc gggcaggggc atagtgatga caagtccttg agatacgttg
     6901 cagttataac ccttaatgct agcgttaccg tccgctatcg tctatgttca agttgtctta
     6961 attgccagaa tctaacggct ttcggcaatt actccaaaag ggggcgctct cttttattga
     7021 tcttacgcat cctgtatgat gcaagcagac taactctatc aacgttggta ttatttcccg
     7081 cagacatgac ccttttagca ctcggtatca accataaaac ggcacctgta tcgctgcgag
     7141 aacgtgtatc gttttcgccg gataagctcg atcaggcgct tgacagcctg cttgcgcagc
     7201 cgatggtgca gggcggcgtg gtgctgtcga cgtgcaaccg cacggaactt tatcttagcg
     7261 ttgaagagca ggataacctg caagaggcgt taatccgctg gctttgcgat tatcacaatc
     7321 ttaatgaaga agatctgcgt aaaagcctct actggcatca ggataacgac gcggttagcc
     7381 atttaatgcg tgttgccagc ggcctggatt cactggttct tggggagccg cagatcctcg
     7441 gtcaggttaa aaaagcgttt gccgattcgc aaaaaggtca tatgaaggcc agcgaactgg
     7501 aacgcatgtt ccagaaatct ttctctgtag cgaaacgcgt tcgcactgaa acagatatcg
     7561 gtgccagtgc tgtgtctgtc gcttttgcgg cttgtacgct ggcgcggcag atctttgaat
     7621 cgctctctac ggtcacagtg ttgctggtag gcgcgggcga aaccatcgag ctggtagcgc
     7681 gtcatctgcg cgaacataaa gtacagaaga tgattatcgc caaccgcact cgcgaacgtg
     7741 cccaaattct ggcagatgaa gttggcgcgg aagtgattgc cctgagtgag atcgacgaac
     7801 gtctgcgcga agccgatatc atcatcagtt ccaccgccag cccgttaccg attatcggga
     7861 aaggcatggt ggagcgcgca ttaaaaagtc gtcgtaacca accaatgctg ttggtggata
     7921 ttgccgttcc gcgcgatgtt gagccggaag ttggcaaact ggcgaatgct tatctttata
     7981 gtgtggacga tctgcaaagc atcatttcgc acaacctggc gcagcgtaaa gccgcagcgg
     8041 ttgaggcgga aactattgtc gctcaggaaa ccagcgaatt tatggcgtgg ctgcgagcac
     8101 aaagcgccag cgaaaccatt cgcgagtatc gcagccaggc agagcacgtt cgcgatgagt
     8161 taaccgccaa agcgttagcg gcccttgagc agggcggcga cgcgcaagcc attatgcagg
     8221 atctggcatg gaaactgact aaccgcttga tccatgcgcc aacgaaatca cttcaacagg
     8281 ccgcccgtga cggggataac gaacgcctga atattctgcg cgacagcctc gggctggagt
     8341 agcagtacat cattttcttt ttttacaggg tgcatttacg cctatgaagc cttctatcgt
     8401 tgccaaactg gaagccctgc atgaacgcca tgaagaagtt caggcgttgc tgggtgacgc
     8461 gcaaactatc gccgaccagg aacgttttcg cgcattatca cgcgaatatg cgcagttaag
     8521 tgatgtttcg cgctgtttta ccgactggca acaggttcag gaagatatcg aaaccgcaca
     8581 gatgatgctc gatgatcctg aaatgcgtga gatggcgcag gatgaactgc gcaaagctaa
     8641 agaaaaaagc gagcaactgg aacagcaatt acaggttctg ttactgccaa aagatcctga
     8701 tgacgaacgt aacgccttcc tcgaagtccg agccggaacc ggcggcgacg aagcggcgct
     8761 gttcgcaggc gatctgttcc gtatgtacag ccgttacgcc gaagcccgcc gctggcgggt
     8821 agaaatcatg agcgccagcg agggtgaaca tggtggttat aaagagatca tcgccaaaat
     8881 tagcggtgat ggtgtgtatg gtcgtctgaa atttgaatct ggcggtcatc gcgtgcagcg
     8941 tgttcctgct acggaatcgc agggtcgtat tcatacttct gcttgtaccg ttgcggtaat
     9001 gccagaactg cctgacgcag aactgccgga catcaaccca gcagatttac gcattgatac
     9061 tttccgctcg tcaggggcgg gtggtcagca cgttaacacc accgattcgg caattcgtat
     9121 tactcacttg ccgaccggga ttgttgttga atgtcaggat gaacgttcac aacataaaaa
     9181 caaagcgaag gcgctttcag tgctcggtgc tcgcatccac gctgctgaaa tggcaaaacg
     9241 ccaacaggcc gaagcgtcta cccgtcgtaa cctgctgggg agtggcgatc gcagcgaccg
     9301 taaccgtact tacaatttcc cgcaggggcg cgttaccgat caccgcatca acctgacgct
     9361 ctaccgcctg gatgaagtga tggaaggtaa gctggatatg ctgattgaac cgattatcca
     9421 ggaacatcag gccgaccaac tggcggcgtt gtccgagcag gaataatgga atatcaacac
     9481 tggttacgtg aagcaataag ccaacttcag gcgagcgaaa gcccgcggcg tgatgctgaa
     9541 atcctgctgg agcatgttac cggcagaggg cgtactttta ttctcgcctt tggtgaaacg
     9601 cagctgactg acgaacaatg tcagcaactt gatgcgctac tgacacgtcg tcgcgatggt
     9661 gaacccattg ctcatttaac cggggggcga gaattctggt cgttgccgtt atttgtttcg
     9721 ccagcgacct taattccgcg cccggatacg gagtgtctgg tggagcaggc actggcgcgg
     9781 ttgcctgaac aaccttgccg tattcttgat ctcgggacgg gtaccggggc gattgcgctt
     9841 gcgctggcta gcgagcgccc ggactgcgaa attatcgctg tagatcgtat gcctgatgct
     9901 gtctccctgg cacaacgtaa tgcccagcat ctggcgatca aaaatatcca cattctgcaa
     9961 agcgactggt ttagcgcgct agccgggcag cagtttgcga tgattgtcag caatccgccg
    10021 tatattgacg agcaggaccc acatcttcaa caaggcgatg tccgctttga gccgctcact
    10081 gcgctggttg cggcagacag tggaatggca gacatcgtgc atatcatcga acagtcgcgt
    10141 aacgcgctgg tatccggcgg ctttctgctt ctggaacatg gctggcagca gggcgaagcg
    10201 gtgcgacaag catttatcct cgcggggtat catgacgtcg aaacctgccg tgactatggt
    10261 gataacgagc gcgtaacgct cggccgctat tatcaatgac aagtttttct acactgctta
    10321 gtgttcatct tattagtatc gcgctttctg ttgggctatt aaccttacgt ttctggctac
    10381 gttatcagaa gcatccacag gcatttgctc gctggacgcg cattgtgccg ccggttgtcg
    10441 atacggtgtt attgttaagc ggcattgcgt tgatggctaa agcgcacatc ctgccatttt
    10501 ccgggcaggc acagtggctg actgaaaagc tgtttggagt tatcatttat atcgttttgg
    10561 gttttattgc actcgattat cgtcgtatgc acagtcagca ggcgcgcatt attgccttcc
    10621 cgctggcgtt ggtggtgctg tacatcatca ttaaactcgc caccacaaaa gtaccgttac
    10681 tggggtaagt catgagatcg ttagctgatt tcgaatttaa taaagcgcca ttgtgcgaag
    10741 gcatgatcct ggcttgcgaa gcaatccgcc gcgattttcc ctcgcaagat gtttacgacg
    10801 aactggagcg tctcgttagt ctggcgaagg aagaaatcag ccagcttctg cctttagaag
    10861 agcagctgga aaaactgatc gcgctgtttt acggcgactg gggatttaaa gcctcacgcg
    10921 gcgtttatcg tctttccgat gcattatggc tggaccaggt gttaaagaat cgacagggca
    10981 gtgcggtatc attaggtgcg gttttattat gggtcgcgaa tcgtctcgat ttgccgctgc
    11041 tgccggtgat tttccctacg cagctgatat tgcgcattga atgtccggat ggcgaaattt
    11101 ggctgattaa tccttttaac ggtgaatcgt taagcgaaca tatgctggac gtatggttaa
    11161 agggaaatat cagcccgtcg gcggaactgt tttatgaaga ccttgatgaa gctgataaca
    11221 ttgaggtaat ccgcaaattg ctggatacac tcaaagccat cgttgatgga agaaaatcag
    11281 atggagctgg cgttacgcac cagcgaagct ttattacaat tcaaccctga agatccctat
    11341 gaaattcgcg atcgcgggtt gatttatgcg caactggatt gcgaacacgt tgcgttgaac
    11401 gatttaagtt atttcgttga acagtgtccg gaagacccga tcagcgaaat gatccgtgcg
    11461 caaataaata acatcgcgca taaacatatt gtgctgcatt aattaatcga cattttactc
    11521 aagattaagg cgatcctatg aaacaaaaag tggttagcat tggcgacatc aacgtagcaa
    11581 atgacctgcc gttcgtactg tttggcggta tgaacgtgct ggaatctcgc gatctggcga
    11641 tgcgcatttg cgagcactac gtaactgtga cccagaaact gggtatccct tacgtgttca
    11701 aagcctcttt tgacaaagcc aaccgctcct ccatccactc ttatcgtgga ccgggcctgg
    11761 aagaagggat gaaaatcttc caagagttga agcagacttt tggcgtgaaa attatcaccg
    11821 acgttcacga accaagtcag gcacagcccg ttgctgatgt cgtggatgtg attcagttgc
    11881 cggcgtttct tgctcgccag actgacctgg ttgaagccat ggcgaaaacc ggtgcggtaa
    11941 ttaacgtcaa gaaaccacag tttgtcagcc cgggacagat gggtaatatc gttgataaat
    12001 tcaaagaagg cggcaacgaa aaagtgattc tttgcgatcg tggtgctaac ttcggctatg
    12061 acaacctggt tgtcgatatg ctgggcttca gcattatgaa gaaagtgtct ggtaactcgc
    12121 cggtgatttt cgacgtgacc cacgcactgc aatgccgcga tccgtttggc gcagcttccg
    12181 gtggtcgtcg tgctcaggtg gctgagctgg cacgagccgg tatggcggta ggtctggcgg
    12241 ggctgtttat tgaagcgcat ccggatccgg aacatgcgaa atgtgatggt ccatccgcgc
    12301 tgccgctggc taaactggaa ccgttcctca agcagatgaa agcgattgat gatctggtga
    12361 aaggtttcga agaactggat accagcaagt aatctttttt gcttgaaaaa taaagtctta
    12421 gcgttctgcg ttaagacttt tttcatgggt gccagataca aaaaaggccg caggctgtta
    12481 cccctgcggc cggtttcggg cgcatattgc catcacggca gcctgacgcc cgtttt
//
LOCUS       DADLFA010000123        12053 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000123 DADLFA010000000
VERSION     DADLFA010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12053
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.123"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            16..192
                     /locus_tag="JQH65_002856"
     CDS             16..192
                     /locus_tag="JQH65_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAY6140219.1"
                     /translation="MSALFFFNAVCIVSDNLLIIESFGEMAYNISYLTRVPGTNTLLA
                     CCCLLRPEEVNSEY"
     gene            182..355
                     /locus_tag="JQH65_002857"
     CDS             182..355
                     /locus_tag="JQH65_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HAY6140220.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(404..1476)
                     /locus_tag="JQH65_002858"
                     /pseudo
     CDS             complement(404..1476)
                     /locus_tag="JQH65_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            complement(1548..4292)
                     /gene="torS"
                     /locus_tag="JQH65_002859"
     CDS             complement(1548..4292)
                     /gene="torS"
                     /locus_tag="JQH65_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HAY6140221.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPMLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALY
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSTVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDVPTALMGDPRRIRQVI
                     TNLLSNALRFTDEGQIVLRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            4375..5364
                     /gene="torT"
                     /locus_tag="JQH65_002860"
     CDS             4375..5364
                     /gene="torT"
                     /locus_tag="JQH65_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HAY6140222.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHYTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAGGYSQLATQQAQIDQCKQWGAEAILLGSSTTSF
                     PDLQKQVANLPVIELVNALDDPLVKSRVGVPWFQMGYQPGRYLVQWAHGKPLNVLLMP
                     GPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQEMLERHPEIDVVAG
                     TAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASDQMIWQGELAVEQA
                     IRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFYQHTSAAKK"
     gene            complement(5337..6029)
                     /gene="torR"
                     /locus_tag="JQH65_002861"
     CDS             complement(5337..6029)
                     /gene="torR"
                     /locus_tag="JQH65_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HAY6140223.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGECYFLAADVC"
     gene            6149..7321
                     /gene="torC"
                     /locus_tag="JQH65_002862"
     CDS             6149..7321
                     /gene="torC"
                     /locus_tag="JQH65_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HAY6140224.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDIAGKAHGDKKEEK"
     gene            7321..9894
                     /gene="torA"
                     /locus_tag="JQH65_002863"
                     /pseudo
     CDS             7321..9894
                     /gene="torA"
                     /locus_tag="JQH65_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            9891..10490
                     /gene="torD"
                     /locus_tag="JQH65_002864"
     CDS             9891..10490
                     /gene="torD"
                     /locus_tag="JQH65_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HAY6140225.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVNELENCIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFAARCHQYDSFGFYAALSQLLLVLVECDHQNK"
     gene            complement(10583..10888)
                     /gene="cbpM"
                     /locus_tag="JQH65_002865"
     CDS             complement(10583..10888)
                     /gene="cbpM"
                     /locus_tag="JQH65_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HAY6140226.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(10888..11808)
                     /gene="cbpA"
                     /locus_tag="JQH65_002866"
     CDS             complement(10888..11808)
                     /gene="cbpA"
                     /locus_tag="JQH65_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HAY6140227.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT         2894 a         3093 c         3191 g         2875 t
ORIGIN      
        1 cgtggagcct tttttatatc tgccttattt ttcttcaacg ctgtatgtat agtaagcgat
       61 aacctgttga ttattgaatc tttcggggag atggcttata acatttctta cctgaccagg
      121 gtaccgggaa ccaacacctt actggcgtgt tgctgtcttt taagaccaga agaggttaac
      181 agtgaatatt gaagagttaa aaaaacaagc cgaaacggaa attgccgact ttatcgcgca
      241 aaaaatcgcc gagctgaaca agaatacagg gaaagaagtc tctgaaattc gcttcaccgc
      301 acgagaaaaa atgaccgggc ttgaaagtta tgatgtcaaa atcaaaataa tgtgattttg
      361 tgaacatcac cccgtgcgag gtgatgttcc gcttgttgct aatttagtga tcaatcattg
      421 gcgcttgtgg aattaagcgt cggtacaatt cctccggcac cgggctttgc catactcccg
      481 catacattgc gtaaccaatc accgcgaaca taatccccag aaccagtagc gtcattaacc
      541 agccagacaa cgcaaaagct tttttatttg ccgcaggttt ttgcagtgaa aaggtcaatg
      601 ttgaggctac cggacatgac tctacgcaag tcatacagcc ggtacattcc actgttcgta
      661 cctgaattaa tttatctacc gggatccgtg atgggcaatt ttttgcgcat ttgccacagc
      721 cgatacaact ttcggcattg cgacgaatct taaacggcga taatagcgaa accacgccca
      781 tcagcgcgcc atatgggcaa agataacgac accaggcatg gcgaataaac aggctggcaa
      841 tcagcaaaac ggtcacgctg attaatgtcg cggtccccat atgacgaaag aaatcgagca
      901 tttttacgtc catcaccacg ctgtagggcg acaacataaa atagtgaatc gcctgagcgg
      961 gcatcaataa cgcgatatag agaaaaaaac tcaacagcaa atacttcacg ccgcgcagag
     1021 gaatatccag ccagcgggga aggacacatt gccgaccaaa cagtttgtta ccgagatcgc
     1081 cgattaattc agaaagcgta ccaaccgggc ataaccatga gcaaaaggcc tttttgagta
     1141 atagactgat gacgataaaa gcgaccaata acagcatcgc ggagcgtgga cggacggtaa
     1201 ctgacctgtt gtaaggctat atttcagatt catcagaccg gcaatcggta gccagccttc
     1261 gatacctccc ggtctggcga caaatgtcgt gctacttgcc gtttcgtaat agcgcaccca
     1321 ataccagaac gtgatggcaa tataaatatt cattgccaac agtaataatt gcgtcgcttt
     1381 acgccaggtc gtggcattgc gccagtcatt ccacggtaat ttgccgcccg tcgtgcctgg
     1441 ccgccgctgc cagcgggttc tttttttctc tgccatgatt ttgtcagtcc gttaagttgc
     1501 ataccaaatg ccactatttt agttgttctt aactggctga tattgattca aatcgcgttc
     1561 aggtctttct tatgcaacca tgcttccaga gcggcaacac tgcgtgtaat ttcttcgtgt
     1621 ggaagggggg cagataatgg ctgctgctcc agttgtgcgc atagctggct ggcgatacgc
     1681 attcccaaac tcgagcaact gctttttagc tgatgcgcgg cacgctttat tttctcgcta
     1741 tcctgactgg cgcgggcaat gtcgatttca tcgagaagcg gcagggcatg ttgtgtaaat
     1801 aataccagcc attcgtggat cttctccgtc cccattaact gagcatcttc attgagttgc
     1861 gatacatcca gcgattgatc gttattgact tgcagttgga gatagtgcgc cagtaactga
     1921 ccgagcactt cacgcggcac cggtttaggg ataatcccgc ggaataatga actggtacgc
     1981 tggcgcagcg tttcgtcaat gacatgggcg ctaaagccaa tcaaaaccag cgacggatat
     2041 tgctgtgcca gttgtcgggc aagcgtaatg ccgtcgacat ccggcagatc aaaatccacc
     2101 agtgcggcag caaacggttc gctattttgc agtgtctcta aagcctgcgc ggcattgcca
     2161 acagcaacaa cctgcgcacc actggtgttc agcatctcaa cggtaattcg ctgggttagc
     2221 gggttatctt caattaacag caaacgtaaa ccgtcaagac gcaccgcctg attgactgtt
     2281 tttggcacgg gtgccgtggc aacacgtaac ggcaagcgta aacaaaaaca gcttccaacc
     2341 tccggcgtgc tggtggcgct cagttcgccg cccatcgcct gggccagacg gctactgata
     2401 gtcagtccca gcccggtgcc gccgcgtttg ccgcttacct gcacaaatgg ctggaagatt
     2461 tctgccagtt tcgcgggatc aataccgcag ccgctgtctt ccacttcgac cagccattgc
     2521 tcgccatcag tgcggctacg taaaacgatc tgcccttcgt cagtaaaacg cagggcgttg
     2581 ctcaacaggt tggttataac ctgacgaata cgtcgcggat cgcccattaa cgcggtcggt
     2641 acatcgtcgg caattgctgt tgccaggcga atcgggcgac ctttcacccg tccgctcatt
     2701 aattgcaggg tactttccag cagcgggcgc ggttcaaagg gctcatcgct gaccgaaaca
     2761 ttcttgccac ctgcttcgat agccgaataa tcgagaatat cgttgaggat ggtcagcaac
     2821 gattcgccag agtcggtaat cgcccgcaaa tcatcacgct gggcgttaag tgcggggtta
     2881 tctgccagca gttgagcagt gccgagaata ccgtacagcg gtgtgcggat ctcatggctc
     2941 atcgccgcca gaaaggccga ttttgcctgg ctggcttttt ctgcttccgc ccgtgcctgt
     3001 cggtgttcta tcaccagttc ctgcaattca gctgtacgcg ctttgacctg cgccgccagc
     3061 tgttcacggt ggcgattcag tgcatgaaca gtgctgcgaa acgcatccat cagccgcccg
     3121 atggtatcca gctcccgtac gccagcggtt tccgggaaag gggagtcaat atcaccgtcc
     3181 agcagccgtt gcagcgcctg cgtttgttcg gcaagtggac gcgtgactga gcgataaacc
     3241 acgcgccaaa ggatcagaat cagtgcgcaa agtgaaacca tccccagcaa taacaggctg
     3301 tattgcccgc gtgcactggc tttttccaga tgcgccagtc cgtgctgatt acgcagctca
     3361 atggtgtcga ccagctgact gacttcgcta ctaaactgcg cgaactgggc gatgttattt
     3421 tgtgcgagag tttgtaggtg attgctgatt tcactgtcct gctgatacag cgccagcaaa
     3481 tcgctatatt ggctaacggt agttaacgtt gttgcgacct gcgcacgaac acccggatct
     3541 tcaatgcgta tttgccgacg ttgcagaatt ttcaccgcat tattgagctg cttttccagc
     3601 attggtgcat ttttctggat ctgctccagc cccagattca tcaccatttg ctgcacccgc
     3661 agagcgctaa ggcgcagttc attcatctgg ttaacatact caagatcgat atcaatcagc
     3721 cgatcgagtg cactttcagc agcctgacgc tgatcttgtt cgatcaaatc gtaaatcccg
     3781 gcctgggtcg ctccagcgga agttgtcgca ttattcgcct gaccttgcgc caggcgtgcg
     3841 atctcatcgg cggcagcgac tatctgctga ctgagttgct gttgttgctg gcgtagttgc
     3901 agacgccgcc ccaccagttc cccttgctga cgtaacgaac gggagatctc ctgctcctgt
     3961 tgttcaatag cggtggtatc aaaaccttgt tcccgtaacg cttgcagcaa cgcattaatc
     4021 ttcaggcttt gtgcggtgag cattcgcccc tgcgcctgcc acatcttttc gttatcggca
     4081 ctggtcaggt tctgcgcggc gaaaagttcc caggcgctgg cttcgctcaa ctggcgcgcc
     4141 atattcatgg taggaatcaa tgcctgagtg ttgtcttttt ccacctggct gataaagcgc
     4201 aggttgtacc atcccaccag ggtactggtc agggttaaca gcgccatcag ggcaaagccc
     4261 atccagagtc ttcgggtcag ggttaaattc acggtcggtg cactttaggt gaaaaaggtt
     4321 gagtcgcaaa gcagaatgca tctagcataa agccttatta ttgattaggc tatcatgcgc
     4381 gtactgctat ttttacttct ttcccttttc atgttatcgg cattttcggc tgataacctg
     4441 ttgcgctggc atgatgcgca gcattacacg gtgcaagcct ctacgccgct taaagccaaa
     4501 cgcgcatgga aactgtgcgc gctttatccc agcctgaaag attcatactg gttatcgttg
     4561 aactatggta tgcaggaggc aggcggctac agccagttgg ctacccagca agcacaaatc
     4621 gaccagtgta aacagtgggg cgcagaggcc attttgctcg gtagtagcac tacgtcattt
     4681 cccgacctgc aaaagcaggt agcaaatctg ccggtgatcg aactggtaaa tgctcttgat
     4741 gatcccctgg tgaaaagccg cgttggtgta ccctggtttc agatgggcta tcaacctggg
     4801 cgatatttgg tgcaatgggc gcacggtaaa ccactgaacg tgctgttgat gcccggaccc
     4861 gataatgccg ggggcagtaa agagatggtc gagggttttc gcgcagccat tgccggaagc
     4921 ccggtgcgta ttgttgatat tgcgcttggt gataacgata ttgaaatcca gcgtaacctg
     4981 ttgcaggaga tgctggaacg ccacccagaa atcgacgtcg ttgccggaac ggccattgcg
     5041 gcagaggcgg caatgggtga agggcgtaac ctgaaaacgc cgcttaccgt ggtgtcgttt
     5101 tatctttcac atcaggtgta tcgcgggctg aagcggggaa gagtgattat ggctgccagc
     5161 gatcaaatga tctggcaggg ggaactggcg gttgagcagg ccatcaggca attacagggg
     5221 caatcggtgt ctgataatgt cagcccaccg attttagttc tgacgccgaa aaatgccgac
     5281 cgtgaacata ttcgccgctc gctgtcacca gggggatttc gtccggtcta tttttatcag
     5341 cacacatcag cggctaagaa ataacattca ccatgttgcg tcaccagtaa atccgcgctg
     5401 agtttatgac gtaaacgacg aattaacaca tcgacggtgc gcaggtcagg gttttccacc
     5461 cgacgcgcag aaagcatacg tagcagacgt tcacggctga gaatttcgcc cggattcgtc
     5521 acaaatgcca ccaacatttc atactctgcg cgggtcagtt taatcggctc gccatcccgc
     5581 tccagcgtat ggcgcgacac attcaggcaa taaccggcaa agcgatagca gttgtcctga
     5641 gtgtgcggtt gagcttgtcg cgcgaggtcg attcgccaga gcagattttt cacccgtact
     5701 accagttcgc gcagttccag cggtttggtg acgtaatcgt ctgcgcccat ttccagccca
     5761 acaatacggt caatccgatc gctgcgtccg gtaaccagaa taatccccac cgttgagcgt
     5821 tctcgcaggg cgcgggttaa catcaggcca ttttcatcgg gtaagttgat atccagcaga
     5881 attaaatcta ccggctgatt ctgcataatt tcccgcagcc cggcaccgct cgctgtaacg
     5941 gaaacggtat acccctcctg agtgaagtaa gattgtaatc gcgcctgggt aaccggctca
     6001 tcttcaacaa taacaatgtg atgtggcatc agagggtttt actcattctg ttcatattct
     6061 gccgtaagcc gttcatcctg accagtgccg ctgttcatat ttgctcatta agatcgcttc
     6121 actaaaccat aattttacag gggctattat gcggaaactc tggaacgcgc tacgccgacc
     6181 cagtgctcgt tggtcggtac tggcgctggt cgcaattggg attgtgattg gcattgcgct
     6241 gattgtattg ccacacgttg ggatcaaagt caccagcaca accgaatttt gtgtcagttg
     6301 ccacagtatg caaccggtgt atgaagaata taaacagtcg gtgcatttcc agaacgcctc
     6361 cggcgtgcga gctgaatgcc atgactgtca tatcccgccg gatattccag gcatggtgaa
     6421 gcgcaaactg gaagcgagca atgatatcta ccagaccttt attgctcact ccattgatac
     6481 acctgaaaaa ttcgaagcca aacgcgcgga acttgccgag cgtgaatggg cgcgaatgaa
     6541 agaaaacaac tcggcaacct gccgctcctg ccataactac gatgcgatgg atcatgcgaa
     6601 gcagcatcct gaagcggcac gtcagatgaa ggtggcagcg aaagataatc aatcctgcat
     6661 cgactgtcat aaaggtattg cccaccagtt accggatatg agtagcggct tccgtaagca
     6721 gttcgatgag ctgcgcgcca gtgctaatga cagtggtgac acgctgtact ctattgatat
     6781 taagccaatt tatgcggcga aaggcgataa agaagcctct ggttctctgc tgcctgcttc
     6841 ggaagtgaaa gtccttaaac gtgacggcga ctggctgcaa attgaaatta ccggctggac
     6901 ggaaagcgcc ggacgtcagc gtgtactcac ccaattccca ggtaaacgca tctttgttgc
     6961 ctcgattcgt ggtgatgtgc agcagcaggt aaaaacgctg gagaaaacca ccgttgccga
     7021 caccaatacc gagtggagca agttgcaggc cactgcgtgg atgaagaaag gtgatatggt
     7081 gaacgatatc aaaccgatct gggcttatgc ggattcgttg tacaacggca cctgtaacca
     7141 gtgccacggc gcaccggaaa tctctcactt tgatgctaac ggctggattg gcacgctcaa
     7201 cggcatgatt ggctttacca gtctcgataa acgtgaagaa cgcaccttgt tgaaatatct
     7261 gcaaatgaat gcgtctgaca tcgcaggtaa ggctcacggc gataagaagg aagaaaaata
     7321 atgaacaata acgatctctt tcaggcatca cgtcggcgtt ttctggcaca actcggcggc
     7381 ttaacagtcg ccgggatgct ggggccgtca ttgttaacgc cgcgcagtgc gactgcggcg
     7441 caagcggcgg atgtcatctc gaaagagggc attcttaccg ggtcgcactg gggggctatc
     7501 cgcgcgacgg tgaaggatgg tcgctttgtg gcggcaaaac cgttcgaact ggataaatat
     7561 ccgtcgaaaa tgattgccgg attgccggat tgccggattg ccggattgcc ggattgccgg
     7621 atcacgtaca caacgcggcg cgtattcgtt atccgatggt acgcgtggac tggctgcgta
     7681 agcgccatct cagcgatacc tcccagcgcg gtgataaccg ttttgtgcgc gtgagctggg
     7741 atgaagccct cgacatgttc tatgaagaac tggaacgcgt gcagaaaact cacgggccga
     7801 gtgccttgct gaccgccagt ggttggcaat cgacggggat gttccataac gcttcgggga
     7861 tgctggcgaa agctattgcc ttgcatggta atagcgttgg tacgggcgga gattactcta
     7921 ccggtgctgc gcaggtgatc ctgccgcgcg tagtcggttc gatggaagtg tatgaacagc
     7981 aaacctcctg gccgctggta ttgcagaaca gcaaaaccat tatgctgtgg ggctccgatt
     8041 tgctgaaaaa ccagcaagcg aactggtggt gcccggatca cgatgtttat gaatattacg
     8101 agcaactaaa agcgaaagtc gccgccggtg aaattgaggt catcagcatc gatccggttg
     8161 tcacatccac ccatgagtat ctggggcgcg agcatgtgaa gcacattgcg gttaacccgc
     8221 aaactgacgt gccgctgcaa ctggcgctgg cgtatacgct gtacagtgaa aacctgtacg
     8281 acaaaaactt cctcgctaac tactgtgtag gttttgagca gttcctgccg tatctgctgg
     8341 gtgagaaaga cggtcagccg aaagatgccg catgggctga aaaactgacc ggcattgatg
     8401 ccgaaaccat tcgtgggctg gcgcggcaga tggcggcgaa cagaacgcaa attattgctg
     8461 gctggtgcgt gcagcgtatg cagcacggtg aacagtgggc gtggatgatt gtggttctgg
     8521 cggcgatgct ggggcaaatt ggcctgccag gtggtggttt tggttttggc tggcactaca
     8581 acggcgcagg cacgccgggg cgtaaaggcg ttattctgag tggtttctcc ggctctacgt
     8641 cgattccgcc tgttcacgac aacagtgact acaaaggcta cagcagcacc attccgattg
     8701 cccgttttat cgatgcgatc ctcgaaccgg ggaaagtgat caactggaac ggtaaatcgg
     8761 taaaactgcc gccactgaaa atgtgtattt ttgccggaac taacccattc catcgccatc
     8821 agcagatcaa ccgcattatt taaggctggc gcaagctgga aacggttatc gccatagata
     8881 accagtggac ctcaacctgc cgctttgccg atatcgtact gcctgcgacc acgcagtttg
     8941 agcgtaacga tctcgaccag tacggcaacc actccaaccg tggcattatc gccatgaaac
     9001 atggtggtgc cgccgcagtt cgaggcgcgc aacgactttg atattttccg cgagctgtgc
     9061 cgtcgcttta atcgcgaaga agcctttacc gaagggctgg acgaaatggg ctggctgaaa
     9121 cgcatctggc aggaaggtgt acaacaaggc aaaggacgcg gcgttcatct gccagcgttt
     9181 gatgacttct ggaataacaa agagtacgtc gagtttgacc atccgcagat gtttgttcgc
     9241 caccaggcat tccgcgaaga tccggatctc gaaccgctgg gcacgccgag tggcctgatt
     9301 gagatctact cgaaaaccat cgccgatatg aactacgacg attgtcaggg gcatccgatg
     9361 tggtttgaga aaatcgaacg ctcccacggt gggcccggct cgcagaagta tccgttgcat
     9421 ctgcaatctg tgcatccgga tttccgactt cactcgcagt tatgtgagtc ggaaactctg
     9481 cgtcagcaat atacggtagc gggtaaagag ccagtattca ttaacccgca ggatgccagc
     9541 gcgcgcggta ttcgtaacgg tgatgtggta cgcgtcttta acgctcgcgg tcaggtgttg
     9601 gcaggggcag tagtttctga ccgctatgca cccggcgtgg cacgaattca cgaaggggca
     9661 tggtacgatc cagataaagg cggcgagcct ggtgcgctgt gcaaatacgg taaccccaac
     9721 gtgctgacca tcgacatcgg tacttcgcag ctggcgcagg cgaccagtgc gcacactacg
     9781 ctggtggaaa ttgagaagta caatggagca gtggagcagg tgacggcgtt taacggccct
     9841 gtggagatag tggcgcagtg cgaatatgtt cccgcgtcgc aggtgaaatc atgaccacgc
     9901 tgacagcaca acagattgcc tgtgtttacg cctggctggc gcagttgttc tcccgtgaac
     9961 tggacgatga acaactgacg caaatcgcca gtgcgcagat ggcggaatgg ttttcgttgc
    10021 tgaaaagcga accgccgctg gctgcggcgg tgaatgagct ggaaaactgt attgccacgc
    10081 tgacagtacg tgacgatgcc cgtctggaac tggccgctga cttttgcggc ctgtttttga
    10141 tgactgataa gcaggcggcg ctgccgtatg catcggccta caaacaggac gagcaagaga
    10201 ttaaacgctt gttagttgag gcagggatgg aaaccagcgg caatttcaac gaaccggcag
    10261 accatctggc gatctatctc gagttgttga gtcatctgca tttttcgctg ggagagggga
    10321 ccgttcctgc gcgaagaatc gacagtttgc ggcaaaaaac actgacggcg ctgtggcaat
    10381 ggttaccaga gtttgctgcg cgttgccatc agtatgacag ctttggtttt tacgcggcac
    10441 taagccagtt attgctggtg ttagtggagt gcgaccacca aaacaaataa cgtcgtttat
    10501 gcgcctgaaa agacgcgttt agcgtcgcat caggcattat ggcgcagttg ccggatgcgt
    10561 catcacatcc ggcaaacaca catcacggat gagctacaaa ccgggaaagc cgctggcgta
    10621 gcaggcggtt ttcctgcttc aggtgtgcaa tatcatccat taacgtcagc gccaccgcga
    10681 tccccggcca gtccagagcc agttcatgac gcaggcgtac cgcgcgttgc accacaatgg
    10741 cggcatggtc gtcaaatacc caggttgttt cctggatctc acgcggttca accaccccca
    10801 aaccgacaat ttcattcaac tcctcttcag agatgccggt atgcaggcaa aattcggtaa
    10861 tagtaaaagt caccgtaaca ttagccatca tgctttcccc caatctttac gtggatcaaa
    10921 agacgactgg gcgtctgcca gttgctgcca cagcgcggca gtgttttcat ccggtttcgg
    10981 cggcatcacg attttcagta ccgcatacag atcgccggtc tgttttttgc tcaccagacc
    11041 tttgccttta acgcgcaatc gttgcccggc ctggctgcct ggcgggatag tcagcaaaat
    11101 gctttctttc agtgttggaa cggtgacttt agcacccagc gccgcttccc acgggctaac
    11161 cggcaccaca atttccagat cctggccgac aatatcgaac agcggatgtg gcgcaatatg
    11221 aatcaccagc cacaaatcgc catttggacc accgttttcg cccggcgtcc cctggccttt
    11281 cagacggatg cgttgaccat tgccgacgcc cgccgggatc ttcacattca gcgttttcgg
    11341 gatttcctgt tcgatcatgc caaaggcgtt ataaaccggc aggttatagc tgatggtacg
    11401 cttatgctca gtcagcgttt cttcgaggaa taccgccact tcgatttcaa tatcgtggcc
    11461 gcgtgcggcg gggcgttgac ggctctggcg ggcatgctga ccgaaaattg acgagaagat
    11521 atcgtcaaaa tcttcggcat taaaactctg accgtcgcca tgatggaact gacggttaaa
    11581 ttgcggatcg ttgcgatgtt gccacatctg atcatactca gcgcgacgtt gttcatcact
    11641 taacacttcc caggcttcag cgacctcttt gaagcgggct tcggcatccg gttctttgct
    11701 gacatcagga tggtatttac gggccagtcg acgataggcg gtcttgattg tcttgagatc
    11761 gtccgtcggt ttcacgccca tgatggcgta ataatccttt aattccatag cgttatctcg
    11821 cgtaaatcaa cacaaattga aggaacccct gtaaggtaac tcctataagt gtagggtaat
    11881 cctcaaaatt tcatatgcca acacagaata tgttattgaa atcatcgtgg agaggaggtc
    11941 gccatcaata tgggttgctg aacatatttt aaacaggtga aaaagggtga gcgatttttg
    12001 atagttgaac caggcacttt aagtttaact agggcgtcat tatttattaa att
//
LOCUS       DADLFA010000124        12039 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000124 DADLFA010000000
VERSION     DADLFA010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12039
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.124"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..410)
                     /locus_tag="JQH65_002867"
     CDS             complement(<1..410)
                     /locus_tag="JQH65_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HAY6140228.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYVLTTQVEFTDPEIVAEYITYPSPNGHGEVWGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVTKAGYIALAPDGLSSV"
     gene            566..1606
                     /gene="gpr"
                     /locus_tag="JQH65_002868"
     CDS             566..1606
                     /gene="gpr"
                     /locus_tag="JQH65_002868"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAY6140229.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGVSRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(1646..2140)
                     /locus_tag="JQH65_002869"
     CDS             complement(1646..2140)
                     /locus_tag="JQH65_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HAY6140230.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            2331..3215
                     /gene="yghA"
                     /locus_tag="JQH65_002870"
     CDS             2331..3215
                     /gene="yghA"
                     /locus_tag="JQH65_002870"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HAY6140231.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRVAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(3487..3912)
                     /gene="exbD"
                     /locus_tag="JQH65_002871"
     CDS             complement(3487..3912)
                     /gene="exbD"
                     /locus_tag="JQH65_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HAY6140232.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(3919..4653)
                     /gene="exbB"
                     /locus_tag="JQH65_002872"
     CDS             complement(3919..4653)
                     /gene="exbB"
                     /locus_tag="JQH65_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HAY6140233.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLFNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            4905..6092
                     /gene="metC"
                     /locus_tag="JQH65_002873"
     CDS             4905..6092
                     /gene="metC"
                     /locus_tag="JQH65_002873"
                     /EC_number="4.4.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HAY6140234.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            6232..6891
                     /locus_tag="JQH65_002874"
     CDS             6232..6891
                     /locus_tag="JQH65_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY6140235.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGMLFVVIKKKYCN
                     A"
     gene            complement(6931..7830)
                     /locus_tag="JQH65_002875"
     CDS             complement(6931..7830)
                     /locus_tag="JQH65_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003024513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY6140236.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            8024..9187
                     /gene="yqhD"
                     /locus_tag="JQH65_002876"
     CDS             8024..9187
                     /gene="yqhD"
                     /locus_tag="JQH65_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HAY6140237.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAADNYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            9292..10119
                     /gene="dkgA"
                     /locus_tag="JQH65_002877"
     CDS             9292..10119
                     /gene="dkgA"
                     /locus_tag="JQH65_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HAY6140238.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVIIAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            10319..11245
                     /locus_tag="JQH65_002878"
     CDS             10319..11245
                     /locus_tag="JQH65_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HAY6140239.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            11296..11553
                     /gene="yqhH"
                     /locus_tag="JQH65_002879"
     CDS             11296..11553
                     /gene="yqhH"
                     /locus_tag="JQH65_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HAY6140240.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            11633..>12039
                     /locus_tag="JQH65_002880"
     CDS             11633..>12039
                     /locus_tag="JQH65_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6140241.1"
                     /translation="MRRWHTCFFILLFCGFSGNVLCKESINEAIERLYQPLTSNDDIP
                     ITDEFSLDLRSNMSATPGKMTPWLNNNLLCRCVDDAFVSAEIIQSTHLNGGRTHVLLQ
                     LVIKQNRTDIHRLLGLMLVRENDNHRWLIEDID"
BASE COUNT         3117 a         3050 c         3001 g         2871 t
ORIGIN      
        1 acggaactta agccgtcagg tgccagggcg atataccccg ccttcgtcac tcgccgtgcc
       61 acatcttcga tatacggatt cagtccacga ttctcatgca ccaccaccac ggctggcgtt
      121 ttgccgctca tttttgcggg tttcaccaga taaccccata cctcgccgtg accatttggc
      181 gaaggatacg tgatgtactc agcaacaatc tccgggtcgg taaactctac ctgagtcgtc
      241 agcacataat ttggcttgag caaatcaaac aacgctaatg ccgtcatccc gcccaccgca
      301 tacttcgccg caaggttgag gaactcacgt ttcgaaattt tcccgtgagc gtaatagtcg
      361 tagtaatcca acaactcttg tgggaaatct ttggcggtta aacgcggcat cgttgcattc
      421 ctcaactggt gttttttaag caaagcataa gcgcgtgttt ttgcccagtt tttcgtcact
      481 ctgtgagcca gactacggga tacgcgctgg cgaatcgcta aactagaaac attgtttcga
      541 aattgaacgg tggaaaggag aggtcatggt ctggttagcg aatcccgaac gttacgggca
      601 gatgcagtac cgctattgcg gaaaaagtgg tttacgcctg cccgcgttat cgctcggttt
      661 atggcacaat ttcggtcacg ttaacgcgct ggaatcacag cgtgcaatcc tgcgtaaagc
      721 gtttgattta ggcattacgc actttgattt agccaacaat tacgggccgc ctccaggaag
      781 cgcagaagag aactttggtc gcctgctgcg ggaggatttt gccgcttatc gcgatgaact
      841 gattatctct accaaggctg gctacgatat gtggcccggc ccttacggct ctggcggttc
      901 acgtaaatac ctgctcgcca gcctcgacca aagcctgaag cgtatggggc ttgagtatgt
      961 cgatatcttt tactctcatc gcgtcgatga aaatacgccg atggaagaaa ccgcctctgc
     1021 gctggctcat gcggtacaaa gcggtaaggc gctgtatgtc gggatctcct cttactcgcc
     1081 agagcggacg caaaaaatgg tcgagttgct gcacgagtgg aaaattccgc ttttaattca
     1141 tcaaccttcg tacaatttac tgaaccgctg ggtggataaa agcggcctgc tggataccct
     1201 gcaaaataac ggcgtgggct gtattgcctt tactcctctg gctcagggat tgctgaccgg
     1261 aaaatatctc aacggcattc cgcaagattc acggatgcat cgtgaaggga ataaagttcg
     1321 tggtctgacg ccgaaaatgc ttaccgaagc caacctcaac agcctgcgct tattgaatga
     1381 aatggcacag cagcgtggac aatcaatggc gcaaatggcg ttaagctggt tgctgaaaga
     1441 tgatcgcgtg acgtcggtat tgattggtgt cagccgcgcg gagcaactag aggagaacgt
     1501 gcaggcgctg aataatctga catttagcac cgaggagctg gcgcagattg atcagcatat
     1561 cgccgatggc gagctgaatc tgtggcaggc gtcttccgat aaatgacctg ttaataacgg
     1621 gcgcgcgaac cgcgcccgaa tcagatcagt gattatgacg agtcagtcgg tcaagatagc
     1681 ccatcacaaa tgcagagagc acaaacgtca gatggataat gacgtaccac atcagtttgt
     1741 tatccgggac atttttcgca tccataaaga cgcgcagtaa gtgaatggaa gaaattgcca
     1801 caatcgacgc tgctactttg tttttcagcg acgttgcgtc cattttcccc agccagttca
     1861 gcttctcttt gttctcggag atatccagct gcgagacgaa attctcataa ccggaaaaca
     1921 tcaccatcac cagcaaaccg ccaaccagcg tcatatccac cagcgacagc aacacgagga
     1981 tcaaatctga ttccgccatc gagaagatat tcggcagtac gtgaataatc tcctggaaga
     2041 acttcagcgc cagggcaact aacgccagcg aaaggccaaa gtacacgggg gcaagcagcc
     2101 agcgagaagc atacattgca ttttcaagaa aacgttccat acattcctgt cgtgtagtta
     2161 aaacgggcaa cagtatatcg caattgagta aacagcacgc aacagcaaat caacatatac
     2221 gttaaacctt tgtcgcataa ttctgaggtt tagggatctt cctggttccc ttatcaacgc
     2281 atcgagagcg tctaccctta aaagacgttg aacctgaagg gagaaaaaca atgtctcatt
     2341 taaaagaccc gactacgcag tattacactg gtgaatatcc caaacagaaa caaccgacgc
     2401 caggcatcca ggcgaagatg acaccggtac cggattgcgg cgagaaaacc tatgttggta
     2461 gcggtcgcct gaaagatcgt aaagcactgg tgacaggggg cgattccgga ataggtcgcg
     2521 ttgccgccat cgcttacgcg cgtgaagggg ctgacgtggc gatcagttat cttcccgtgg
     2581 aagaagaaga cgctcaggat gtgaaaaaga tcattgaaga atgcggacgc aaagccgttc
     2641 tgctgccagg cgatttaagc gatgagaaat tcgcccgttc gctggttcac gaagcgcaca
     2701 aggcgttagg cgggctggat attatggcgc tggtcgccgg gaaacaggtt gccattccgg
     2761 atattgcaga cctcaccagc gaacagtttc aaaagacctt tgccattaac gttttcgcgc
     2821 tgttctggct aacccaggaa gcgatccccc tgctaccgaa aggtgcaagt atcatcacca
     2881 cttcgtcaat ccaggcatac cagccaagtc cgcatttact ggactatgcg gctacgaagg
     2941 cggcgattct gaactacagc cgtggcctgg caaaacaggt cgcggagaaa ggtattcggg
     3001 tgaatattgt cgcgccaggc cctatctgga cggcactgca aatttccggc ggacaaacgc
     3061 aggataagat cccgcagttt ggtcagcaaa cgccgatgaa gcgtgcgggg caaccggcgg
     3121 aactggcccc tgtatatgtt tatctggcaa gtcaggagtc gagctacgtc accgcagaag
     3181 tgcacggtgt gtgcggcggc gagcatttag gttaaaaaaa tgcccggttg tgaaaagcaa
     3241 ccgggcatca ttgtgaattc actcttacct gatgtggcga aaacgcctta tccgcctaca
     3301 aacgcatgaa gattcaacat gttgcaatag attttgtagg cctgataagc gtagcgcatc
     3361 aggcattgag cacctaatgc cggatgcggc gtgaacgcct tatccggcct acaaacgcac
     3421 gaagattcaa catgttgcaa tagattttgt aggcctgatg agcgtagcgc atcaggcaat
     3481 tctactttac ttcgctttgg cggtttcttc gccgaccaga cctatcttca ggtaacccgc
     3541 ctgatgcagc gtatccatta ccttcatcaa cgtctcgtaa tcgacggttt tatccgctcg
     3601 gaagaagatg gtggtgtctt tcttgccttc ggttaacgca ttcaacgccg taatcattgt
     3661 ttcatcggtg accggatcgt taccgataaa catcgagttg tctgccttca ccgacagata
     3721 aaccggtttt tccgggcgcg gctgcggcgt gctggtagaa gcaggcaagt tcaccttcac
     3781 atctaccgtc gctaacggtg ccgccaccat aaagataatc agcagaacca acatcacgtc
     3841 gataaacggc gtcacgttga tatcatgcat ttcgccgtta tcgtcgaggt tttcgttaag
     3901 atgcattgcc atcggatatt atcctgcgcg taatttttgt gcgacacgaa ccggatgcgc
     3961 agcggcgctg gcttccagat ccaggtcacg gctttgcagc aacaatacct gcgctgcaac
     4021 atcacccagc atcgctttaa agccgccaat ctggcgtgca aatacgttat agataacgac
     4081 cgcaggaatc gctgccacga ggccgattgc cgttgctaac agagcttctg cgatacccgg
     4141 cgcaacgact gccaggttag tggtctgcgt ttgcgcgata ccaataaagc tgttcatgat
     4201 gccccagacc gtaccaaaca gaccaacgaa cggagaaatc gcgccgatgg ttgccagata
     4261 gccgttaccg cggcccattt gacgacccac tgcggcgacc cgacgctcca gacggaagct
     4321 ggtacgttct ttaatacctt cgttatcgtc gctgccttct gacagttcca gctcattctg
     4381 cgcttcattg aacaaatgca ggcttaagct tttgctacca aaatcagcgg cgatatcgtt
     4441 ggcctggttt aaggaacgcg cttcagccag cagttgctgc tcgcgcttta ggcgacgctt
     4501 ctgattgaag aactctacgc tcttactgaa gaagattgcc caggtgacta cggaggccaa
     4561 aataagccca atcatcacgc acttaacgac aatatcggcg tgctgataca taccccagac
     4621 ggaaaggtct gtctgcatta aattattacc cactgtgtat ctccaggacg caagtcacaa
     4681 aatctgcgca taataatatc aaaacgacgt cgaattgata gtcattctca ttactatttg
     4741 catactgccg tacctttgct ttcttttcct tgcgtttacg cagtaaaaaa gtcaccagca
     4801 cgccatttgc gaaaattttc tgctttatgc caattcttca ggatgcgccc gcgaatattc
     4861 atgctagttt agacatccag acgtataaaa acaggaatcc cgacatggcg gacaaaaagc
     4921 ttgatactca actggtgaat gcaggacgca gcaaaaaata cactcttggc gcggtaaata
     4981 gcgtgattca gcgcgcttct tcgctggtat ttgagagtat ggaagccaaa aagcacgcga
     5041 cgcgcaatcg cgccaatggc gagttgttct atggacggcg cggaacgtta acccattttt
     5101 ccttacaaca agcgatgtgt gaactggaag gtggcgcagg ctgcgcgcta tttccctgcg
     5161 gggcggcagc ggttgctaat tcgattcttg cttttgtcga acagggcgat catgtgttga
     5221 tgaccaacac tgcctatgaa ccgagtcagg atttctgtag caaaatcctc agcaaactgg
     5281 gcgtaacgac atcgtggttt gatccgctga ttggtgccga tatcgttaag catctgcagc
     5341 caaacactaa aatcgtgttt ctggaatcgc caggctccat caccatggaa gtccacgacg
     5401 ttccggcgat tgttgccgcc gtacgcagtg tggtgccgga tgccatcatt atgatcgaca
     5461 acacctgggc agccggtgtg ctgtttaagg cactggattt tggcatcgat gtttctattc
     5521 aagccgccac caaatatctg gttgggcatt cagatgcgat gattggcact gccgtgtgca
     5581 atgcccgttg ctgggagcag ctacgggaaa acgcctatct gatgggccag atggtcgatg
     5641 ccgataccgc ctatataacc agccgtggcc tgcgcacatt aggtgtgcgt ttgcgtcaac
     5701 atcatgaaag cagtctgaaa gtggctgaat ggctggcaga acatccgcaa gtagcgcgag
     5761 ttaaccaccc tgctctgcct ggcagtaaag gacacgaatt ctggaaacga gactttacag
     5821 gtagcagcgg gctgttttcc tttgtgctta agaaaaaact cagtaatgaa gagctggcga
     5881 actatctgga taacttcagt ttattcagca tggcctactc gtggggcggg tatgaatcgt
     5941 tgatcctggc gaatcaacca gaacatatcg ccgccattcg cccacaaggc gagatcgatt
     6001 ttagcgggac cttgattcgc ctgcatattg gtctggaaga tgtcgacgat ctgattgccg
     6061 atctggacgc cggttttgcg cgaattgtat aacattgcca cttttggaca attttgcaga
     6121 catttaattg tgaaaagtct taaattgttg cgtccgggat caaggcgtcc cggacgaatc
     6181 aggagtacaa taggcagata aaggcttaaa cgctgttcca caggaaagtc catggctgtt
     6241 attcaagata tcatcgctgc gctctggcaa cacgactttg ccgcgctggc ggatcctcat
     6301 attgttagcg ttgtttactt tgtcatgttt gccacgctgt ttttagaaaa cggcctgctg
     6361 cccgcctcat ttttgccagg cgacagcttg ttgatactgg caggcgcatt gattgcccag
     6421 ggggttatgg attttctgcc tacgattgcg attctgaccg ccgcagcaag tctgggctgc
     6481 tggctaagtt atattcaggg gcgctggtta gggaatacca aaacggtgaa aggctggctg
     6541 gcacagcttc ctgctaaata tcaccagcgc gccacctgca tgtttgaccg ccacggtctg
     6601 ctggcgctgc tggctggacg ttttcttgca tttgtccgta cgctgctgcc aaccatggcg
     6661 ggaatttccg gtctgccaaa ccgtcgcttc cagtttttca actggttaag tggattgctg
     6721 tgggtcagcg tggtaaccag ttttggctat gccttaagta tgattccgtt cgttaaacgc
     6781 catgaagatc aggtaatgac gttcctgatg atcctgccaa ttgccttgtt aaccgctggc
     6841 ttgttaggca tgctgtttgt ggtgattaaa aaaaaatact gtaacgcctg acgattttcc
     6901 ccgttcccgg ctgctgtacc gggaacgtat ttaattcccc tgcatcgccc gcattcttgc
     6961 cgcatcttcc cccggcgtca caccgaagta acgtttaaac tcacggctaa attgcgatgc
     7021 gctttcatag ccgacgcgca tcgctgctgc gctggccttc atgccgtcat ggatgatcat
     7081 catccgcgcc ttatgcagac ggtaattctt caaatactgc aacggcgagg tgctggtgac
     7141 agacttaaaa ttatggtgga acgccgatac gctcatgttg gcttctgccg ccagttgctc
     7201 gacgctcagg ttttcggtgt atttattctc aatccgtttc agcacgcggc taatcagact
     7261 gaagtgagtc tggcgactga ccagcgccag taacgcgccg ccgcaaggtc cggtcagcac
     7321 gtagtacaga atttcgcgga tgatctgttt gccgagaata cgcgcatcca gtggtcgctc
     7381 catcacgtcg agtaaccgct ccgccgcgca taaaatctct tctgataacg tggcggagtt
     7441 aatcccgctg gctgccatcg acggctggaa atgctcatct tcgccaatgt ccatcaacag
     7501 ttcctgtaac tgcaaaatat cgacattgag acgcaaccct gccagcggca cctctgacgt
     7561 ggcataggtt tcgcactcaa acggcaacgg caccgtcagc agcaggtatt cattggcatc
     7621 ataacgaaac acgcgttcat tgatataacc gattttatgc ccggaaaaga gaattatgat
     7681 gccaggctcg tacatcaccg gtgtacgtgc gaaaggcgtc tcaccataca acaaacgcac
     7741 atcgggcaac agttctgaca aactattttc tttatttttc agtttattaa ctttatccgc
     7801 cagcaagcgg caaatctctt cacgtttcat atcgcgtaat ttcttaggaa taatgcggca
     7861 atttgattgt gcgcaatttt gtagcatttc tccagcactc tggagaaata ggcaagacat
     7921 tggcagaaat gagcattgag agccagggcg ctggcgatca caatgaaaaa catcaggcag
     7981 atcgttctct gccctcatat tggcccagca aagggagcaa gtaatgaaca actttaatct
     8041 gcacacccca acccgcattc tgtttggtaa aggcgcaatc gctggtttac gcgaacaaat
     8101 tcctcacgat gctcgcgtat tgattaccta cggtggcggc agcgtgaaaa aaaccggcgt
     8161 tctcgatcaa gttctggatg ccctgaaagg catggacgtg ctggaatttg gcggtattga
     8221 gccaaacccg gcttatgaaa cgctgatgaa cgccgtgaaa ctggttcgcg aacagaaagt
     8281 gactttcctg ctggcggttg gcggcggttc tgtactggac ggcaccaaat ttatcgccgc
     8341 agcggataac tatccggaaa atatcgatcc gtggcacatt ctgcaaacgg gcggtaaaga
     8401 gattaaaagc gccatcccga tgggctgtgt gctgacgctg ccagcaaccg gttcagaatc
     8461 caacgcaggc gcggtgatct cccgtaaaac cactggcgac aagcaggcgt tccattctgc
     8521 ccatgttcag ccggtatttg ccgtgctcga tccggtttat acctacaccc tgccgccgcg
     8581 tcaggtggct aacggcgtag tggacgcctt tgtacacacc gtggaacagt atgttaccaa
     8641 accggttgat gccaaaattc aggaccgttt cgcagaaggc attttgctga cgctgatcga
     8701 agatggtccg aaagccctga aagagccaga aaactacgat gtgcgcgcca acgtcatgtg
     8761 ggcggcgacg caggcgctga acggtttgat tggcgctggc gtaccgcagg actgggcaac
     8821 gcatatgctg ggccacgaac tgactgcgat gcacggtctg gatcacgcgc aaacactggc
     8881 tatcgtcctg cctgcactgt ggaatgaaaa acgcgatacc aagcgcgcta agctgctgca
     8941 atatgctgaa cgcgtctgga acatcactga aggttccgac gatgagcgta ttgacgccgc
     9001 gattgccgca acccgcaatt tctttgagca attaggcgtg ccgacccacc tctccgacta
     9061 cggtctggac ggcagctcca tcccggcttt gctgaaaaaa ctggaagagc acggcatgac
     9121 ccaactgggc gaaaatcatg acattacgtt ggatgtcagc cgccgtatat acgaagccgc
     9181 ccgctaagct ttttacgcct caaactttcg ttttcgggca tttcgtccag acttaagttc
     9241 acaacacctc accggagcct gctccggtga gttcatataa aggaggaacg tatggctaat
     9301 ccaaccgtta ttaagctaca ggatggcaat gtcatgcccc agctgggact gggcgtctgg
     9361 caagcaagta atgaggaagt aatcatcgcc attcaaaaag cgttagaagt gggttatcgc
     9421 tcgattgata ccgccgcggc ctacaagaac gaagaaggtg tcggcaaagc cctgaaaaat
     9481 gcctcagtca acagagaaga actgttcatc accactaagc tgtggaacga cgaccacaag
     9541 cgcccccgcg aagccctgct cgacagcctg aaaaaactcc agcttgatta tatcgacctc
     9601 tacttaatgc actggcccgt tcccgctatc gaccattatg tcgaagcatg gaaaggcatg
     9661 atcgaattgc aaaaagaggg attaatcaaa agcatcggcg tgtgcaactt ccagatccat
     9721 cacctgcaac gcctgattga tgaaactggc gtgacgcctg tgataaacca gatcgaactt
     9781 catccgctga tgcaacaacg ccagctacac gcctggaacg cgacacacaa aatccagacc
     9841 gaatcctgga gcccattagc gcaaggaggg aaaggcgttt tcgatcagaa agtcattcgc
     9901 gatctggcag ataaatacgg caaaaccccg gcgcagattg ttatccgctg gcatctggat
     9961 agcggcctgg tggtgatccc gaaatcggtc acaccttcac gtattgccga aaactttgat
    10021 gtctgggatt tccgtctcga caaagacgaa ctcggcgaaa ttgcaaaact cgatcagggc
    10081 aagcgtctcg gtcccgatcc tgaccagttc ggcggctaac atgcaaattc tcccggtggc
    10141 ggtaatgttc cgctaccgga cttttcagaa atcatttatt cccctcgcgt cccgcccgtt
    10201 gttactcttc cttgttcagg aatgccaaat ataaggacat catcatgcag agccggaagc
    10261 tcttaaaaga acaactcatc tatatccggg ataaacgcaa cggagaggtg aaaaacagat
    10321 gaaaataata cttctgtttt tagcagccct ggcaagtttt accgtacacg cgcagcctcc
    10381 ttcacagacc gtagaacaaa cagtccggca gatttatcag aactataaat cagatgccag
    10441 tacgccttat ttcggtgaaa ccggagagcg ggcgataact tcagcgcgta ttcaacaggc
    10501 gcttaccctg aacgacaatc ttacgctgcc gggcaatatt ggctggctgg attatgatcc
    10561 ggtttgtgat tgtcaggatt ttggcgatct ggtgctagaa agcgttgcga taacccaaac
    10621 tgacgccgat catgccgatg ccgttgtgcg ctttcgtatc tttaaagatg ataaagaaaa
    10681 gaccacgcag acactgaaaa tggtggcgga aaatggtcgt tgggtcattg acgatattgt
    10741 cagcaatcat ggtagtgtct tacaagcagt taatagcgag aatgaaaaaa cgctggccgc
    10801 tttagcttcg ttgcaaaaag aacagccgga agcctttgtt gccgaactct ttgaacatat
    10861 tgctgattat agctggccgt ggacgtgggt ggtttccgac tcttaccgcc aggcggttaa
    10921 tgccttctat aaaaccacct tcaagacggc caataatccc gatgaagata tgcaaataga
    10981 acggcaattt atttacgaca atccgatctg ttttggcgaa gagtcgctat tttcacgcgt
    11041 tgatgaaatt cgagtcctgg agaaaaccgc cgattccgcc cgcattcatg ttcgttttac
    11101 gctgaccaat ggcaacaacg aagagcaaga actggtttta cagcggcgcg aaggcaagtg
    11161 ggaaatcgct gattttatcc gcccgaacag cggcagccta cttaagcaga ttgaggcaaa
    11221 aactgccgcc agattaaagc aatgagctga attaaataac aattagccgg aacaataaat
    11281 aaaagggaac actatatgaa aacgattttc accgtgggag ctgttgttct ggcaacctgc
    11341 ttgctcagtg gctgcgtcaa tgagcaaaag gtcaatcagc tggcgagcaa tgtgcaaaca
    11401 ttaaatgcca aaatcgcccg gcttgagcag gatatgaaag cactacgccc acaaatctat
    11461 gctgccaaat ccgaagctaa cagagccaat acgcgtcttg atgcacagga ctattttgat
    11521 tgcctgcgct gcttgcgtat gtacgcagaa tgataaaaaa atccccggca gcatgtcagt
    11581 tgccgggtta taggctgttt gctaaaaaaa agccaaacat aaggacatga ttatgcgacg
    11641 ctggcacacc tgttttttta ttctgctgtt ctgtggcttc agcggaaacg tgttgtgcaa
    11701 agaatcgatt aatgaagcga tagaacgttt gtaccaacca ttgaccagta atgacgatat
    11761 accaataacc gatgaattca gtctcgattt gcgaagtaat atgagcgcta ctcctggcaa
    11821 aatgacaccc tggctgaata ataatctttt gtgtcgctgt gtcgatgacg ctttcgtttc
    11881 tgcagaaata atccaaagca cacacctaaa tggcggccga acgcatgtac tgttgcagct
    11941 ggttatcaaa caaaatcgca cagatatcca ccggttactg gggctaatgc tggttcgtga
    12001 aaatgataat catcgttggc taatagaaga catcgatga
//
LOCUS       DADLFA010000125        11925 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000125 DADLFA010000000
VERSION     DADLFA010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11925
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.125"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            216..1325
                     /gene="livk"
                     /locus_tag="JQH65_002881"
     CDS             216..1325
                     /gene="livk"
                     /locus_tag="JQH65_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HAY6140242.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1373..2299
                     /gene="livH"
                     /locus_tag="JQH65_002882"
     CDS             1373..2299
                     /gene="livH"
                     /locus_tag="JQH65_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HAY6140243.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            2296..3573
                     /locus_tag="JQH65_002883"
     CDS             2296..3573
                     /locus_tag="JQH65_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivM"
                     /protein_id="HAY6140244.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            3570..4337
                     /gene="livG"
                     /locus_tag="JQH65_002884"
     CDS             3570..4337
                     /gene="livG"
                     /locus_tag="JQH65_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HAY6140245.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4339..5052
                     /gene="livF"
                     /locus_tag="JQH65_002885"
     CDS             4339..5052
                     /gene="livF"
                     /locus_tag="JQH65_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HAY6140246.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRAASGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            5449..6120
                     /locus_tag="JQH65_002886"
     CDS             5449..6120
                     /locus_tag="JQH65_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAY6140247.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENRHLPGDRRRHVRRAGSGH
                     HDADL"
     gene            6050..6253
                     /locus_tag="JQH65_002887"
                     /pseudo
     CDS             6050..6253
                     /locus_tag="JQH65_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="with UgpEC is involved in the uptake of
                     glycerol-3-phosphate; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter permease"
     gene            6250..7095
                     /gene="ugpE"
                     /locus_tag="JQH65_002888"
     CDS             6250..7095
                     /gene="ugpE"
                     /locus_tag="JQH65_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HAY6140248.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATTTF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            7097..8167
                     /locus_tag="JQH65_002889"
     CDS             7097..8167
                     /locus_tag="JQH65_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
                     /protein_id="HAY6140249.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIELPLNGGYRQYAGRKM
                     TLGIRPEHIVLSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            8164..8907
                     /gene="ugpQ"
                     /locus_tag="JQH65_002890"
     CDS             8164..8907
                     /gene="ugpQ"
                     /locus_tag="JQH65_002890"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAY6140250.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVVGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQLAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(8894..9334)
                     /locus_tag="JQH65_002891"
     CDS             complement(8894..9334)
                     /locus_tag="JQH65_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HAY6140251.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            9454..11187
                     /gene="ggt"
                     /locus_tag="JQH65_002892"
     CDS             9454..11187
                     /gene="ggt"
                     /locus_tag="JQH65_002892"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HAY6140252.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAVAAPPVSYGVEEDIFHPVRAK
                     QGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIRSKN
                     GNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDKYGT
                     MPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKKGDK
                     LVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERTPIS
                     GDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAYADR
                     SEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAQPSSEIRPGKLAPYESNQTTHYS
                     VVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGDANA
                     VGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEATNA
                     PRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYGASD
                     PRSVDDLTAGY"
     gene            complement(11225..11509)
                     /locus_tag="JQH65_002893"
                     /pseudo
     CDS             complement(11225..11509)
                     /locus_tag="JQH65_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(11709..11864)
                     /locus_tag="JQH65_002894"
     CDS             complement(11709..11864)
                     /locus_tag="JQH65_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6140253.1"
                     /translation="MEKLTTELHSLSEMDRRHVVSILAEIANGYDDFNDMLIYLEFYP
                     NHKILIS"
BASE COUNT         2707 a         2956 c         3387 g         2875 t
ORIGIN      
        1 aaaggcactt ttttctgttt atctatcaat aaattcagaa tattatctgt tcttaatcga
       61 ctgaaagata gggattttaa tcactattat cacaaaatac tgcgctaaac ccttaatcag
      121 acaggcaaaa acagtgcagt ataaaaaaag aacagtctga tttgttaaca cataaaaaca
      181 aagcaacaca acatcacgaa tggggatttt tgactatgaa acggaatgcg aaaactatca
      241 tcgcagggat gattgcactg gcaatttcac acaccgctat ggctgacgat attaaagtcg
      301 ccgttgtcgg cgcgatgtcc ggcccgattg cccagtgggg cgatatggaa tttaacggcg
      361 cgcgtcaggc gattaaagac attaatgcca aagggggaat taagggcgac aagctggttg
      421 gcgtggaata tgacgacgcc tgcgacccga aacaagccgt tgcggtcgcc aacaaaatcg
      481 ttaatgacgg cattaaatac gttattggtc atctgtgttc ttcttctacc caacctgcat
      541 cagatatcta cgaagacgaa ggtattctga tgatctcacc gggagcgacc aacccggagc
      601 tgacccaacg cggttatcaa cacattatgc gtactgccgg gctggactct tcccaggggc
      661 caacggcggc aaaatacatt cttgagacgg tgaagcccca gcgcatcgcc atcattcacg
      721 acaaacaaca gtatggcgaa gggctggcgc gttcggtgca ggacgggctg aaagcggcta
      781 acgccaacgt cgtcttcttc gacggtatta ccgccgggga gaaagatttc tccgcgctga
      841 tcgcccgcct gaaaaaagaa aacatcgact tcgtttacta cggcggttac tacccggaaa
      901 tggggcagat gctgcgccag gcccgttccg ttggcctgaa aactcagttt atggggccgg
      961 aaggtgtggg taacgcatca ttgtcgaata ttgccggtga tgctgccgaa ggcatgttgg
     1021 tcactatgcc aaaacgctat gaccaggatc cggcaaacca gggcatcgtt gatgcgctga
     1081 aagcagacaa gaaagatccg tccgggcctt atgtctggat cacctacgcg gcggtgcaat
     1141 ctctggcgac tgcccttgag cgtactggca gcgatgagcc gctggcgctg gtgaaagatt
     1201 taaaagctaa cggtgcaaac accgtgattg ggccgctgaa ctgggatgaa aaaggcgatc
     1261 ttaagggatt tgattttggt gtcttccagt ggcacgccga cggttcatcc acggcagcca
     1321 agtgatcatc ccaccgcccg taaaatgcgg gcgggtttag aaaggttacc ttatgtctga
     1381 gcagtttttg tatttcttgc agcagatgtt taacggcgtc acgctgggca gtacctacgc
     1441 gctgatagcc atcggctaca ccatggttta cggcattatc ggcatgatca acttcgccca
     1501 cggcgaggtt tatatgattg gcagctacgt ctcatttatg atcatcgccg cgctgatgat
     1561 gatgggcatt gataccggct ggctgctggt ggcggcggga ttcgtcggcg caatcgtcat
     1621 tgccagcgcc tacggctgga gtatcgaacg ggtagcctat cgcccggtgc gtaactctaa
     1681 gcgcctgatt gcgctcatct cggcaatcgg catgtccatc ttcctgcaaa actacgttag
     1741 cctgactgaa ggttcgcgtg acgtggcgct gcccagtctg tttaacggtc agtgggtggt
     1801 ggggcatagc gaaaacttct ctgcctctat taccaccatg caggcagtga tctggattgt
     1861 tactttcctc gccatgctgg cgctgacgat tttcattcgt tattcccgta tggggcgcgc
     1921 ctgccgtgcc tgcgcggaag atctgaaaat ggcgagcctg cttggcatca acaccgaccg
     1981 ggtgattgct ttgacctttg ttattggcgc ggcgatggcg gcagtggctg gcgtactgct
     2041 cggtcagttc tacggcgtca ttaaccccta tatcggcttt atggccggga tgaaagcctt
     2101 taccgctgcg gtgctcggtg ggattggcag cattccaggg gcgatgattg gtgggctgat
     2161 cctggggatt gcggaggcgc tctcttctgc ctatctgagt acggaatata aggatgtggt
     2221 ttcatttgcc ctgctgattc tggtgctgct ggtgatgccg accggtattc tgggtcgccc
     2281 ggaggtagag aaagtatgaa accgatgcat attgcaatgg cgctgctctc tgccgcgatg
     2341 ttttttgtcc tggcgggcgt ctttatgggc gtgcaactgg agctggatgg caccaaactg
     2401 gtggtcgaca cggcttcaga catccgttgg cagtgggtgt ttatcggcac ggcggtggtc
     2461 tttttcttcc agcttttgcg accggctttc cagaaagggc taaaaagcgt ttccggaccg
     2521 aagtttattc tgcccgcgat tgatggctcc acggtgaagc agaaactgtt cctcgtggcg
     2581 ctgttggtgc ttgcggtggc gtggccgttt atggtgtcac gtgggacggt ggatatcgcc
     2641 actctgacca tgatctacat tatcctcggt ctcgggctga atgtggttgt tggtctttct
     2701 ggtctgctgg tgctggggta cggcggtttt tacgccatcg gcgcttacac ctttgcgctg
     2761 ctcaatcact attacggctt gggcttctgg acttgtctgc cgattgccgg gttaatggca
     2821 gcggcggcgg gcttcctgct cggctttccg gtgctgcgtc tgcgcggtga ttatctggcg
     2881 atcgttaccc tcggcttcgg cgaaattgtg cgcatattgc tgctcaataa caccgaaatt
     2941 accggcggcc cgaacggaat cagtcagatc ccgaaaccga ctttcttcgg ccttgagttc
     3001 agccgtaccg cccgtgaagg cggttgggac acgttcagta atttctttgg cctgaaatac
     3061 gatccctccg atcgcgtcat cttcctgtat ctggtggcgt tgctgctggt ggtgctaagc
     3121 ctgtttgtca ttaaccgcct gctgcggatg ccgctggggc gtgcgtggga agcgttgcgt
     3181 gaagatgaga tcgcctgccg ttcgctgggc ttaagcccgc gtcgtatcaa gctgaccgct
     3241 tttaccatca gtgccgcgtt tgccggtttt gccggaacgc tgtttgctgc gcgccagggc
     3301 tttgtcagcc cggaatcctt cacctttgct gaatcggcgt ttgtactggc gatagtggtg
     3361 ctcggcggta tgggatcgca gtttgcggtg attctggcgg caattttgct ggtggtgtcg
     3421 cgcgagttga tgcgtgattt caacgaatac agcatgttaa tgctcggtgg tttgatggtg
     3481 ctgatgatga tctggcgtcc gcagggcttg ctgcccatga cgcgcccgca actgaagctg
     3541 aaaaacggcg cagcgaaagg agagcaggca tgagtcagcc attattatct gtgaacggcc
     3601 tgatgatgcg cttcggcggc ctgctggcgg tgaacaacgt caatcttgaa ctgtacccgc
     3661 aggagatcgt ctcgttaatc ggtcctaacg gtgccggaaa aaccacggtc tttaactgcc
     3721 tgaccggatt ctacaaaccc actggaggca ccattttact gcgcgatcag catctggaag
     3781 gtttaccggg gcagcaaatt gcccgcatgg gcgtggtgcg taccttccag catgtgcgtc
     3841 tgttccgtga aatgacggtg attgaaaacc tgctggtggc gcagcatcag caactgaaaa
     3901 ccgggctgtt ctctggcctg ttgaaaacgc catccttccg tcgcgcccag agcgaagcgc
     3961 tcgatcgcgc cgcgacctgg cttgagcgca ttggtttgct ggaacacgcc aaccgtcagg
     4021 cgagtaacct ggcctatggt gaccagcgcc gtcttgagat tgcccgctgc atggtgacgc
     4081 agccggagat tttaatgctc gacgaacctg cggcaggtct taacccgaaa gagacgaaag
     4141 agctggatga gctgattgcc gaactgcgca atcatcacaa caccactatc ttgttgattg
     4201 aacacgatat gaagctggtg atgggaattt cggaccgaat ttacgtggtc aatcagggga
     4261 cgccactggc aaacggtacg ccggagcaaa tccgtaataa cccggacgtg atccgtgcct
     4321 atttaggtga ggcataagat ggaaaaagtc atgttgtcct ttgacaaagt cagcgcccac
     4381 tacggcaaaa tccaggcact gcatgaggtg agcctgcata tcaatcaggg cgagattgtc
     4441 acgctgattg gcgcgaacgg ggcggggaaa accaccttgc tcggcacgtt atgcggcgat
     4501 ccgcgcgccg ccagcgggcg aattgtgttt gatgataaag acattaccga ctggcagacg
     4561 gcgaaaatca tgcgcgaagc ggtagcgatt gtcccggaag ggcgtcgcgt cttctcgcgg
     4621 atgacggtgg aagagaacct ggcgatgggc ggcttttttg ccgaacgcga ccagttccag
     4681 gagcgcataa agtgggtgta tgaactgttt ccacgtctgc atgagcgccg tgttcagcgg
     4741 gcaggcacca tgtccggcgg tgagcaacaa atgctggcga ttggtcgcgc gctgatgagc
     4801 aacccgcgtt tgctgctact tgatgagcca tcgctcggtc ttgcgccgat tatcatccag
     4861 caaattttcg acaccatcga gcagctgcgc gagcagggga tgactatctt cctcgtcgag
     4921 cagaacgcca accaggcgct aaagctggcg gatcgcggct acgtgctgga aaacggccat
     4981 gtggtgctct ccgataccgg cgatgcgctg ctggcgaacg aagcagtgag aagtgcgtat
     5041 ttaggcgggt aataacacgt tgattgataa ggagtcaaaa gactcctttg agacaggtga
     5101 caaatgtaaa attgcctgat gcgctgcgct tatcaggcct actgggtgag ttgcaatata
     5161 ttgaatttgc acgatcttgt aggccggata aggcgtttac gccgcatccg gcatgaaacg
     5221 atgagtaatc tgtagagttt gattcagacc ttctattttt ccgcttatcc gtgccccatc
     5281 tcccattttc cctcacccac gccgtcaccg ccttgtcatc tttctgacac cttactatct
     5341 tacaaatgta acaaaaaagt tatttttctg taattcgagc atgtcatgtt accccgcgag
     5401 cataaaacgc gtgaattcgc gcattcggta caacaagaga gataaacgat gaaaccgtta
     5461 cattatacag cttcagcact ggcgctcgga ctggcgttaa tggggaatgc acaggcagtg
     5521 acgaccattc cgttctggca ttctatggaa ggggaactgg gtaaagaggt ggattctctg
     5581 gcccaacgtt ttaacgccga aaatccggat tacaaaattg taccgaccta taaaggcaac
     5641 tacgaacaga atttaagcgc ggggattgcc gcatttcgta ccggcaacgc tccggctatt
     5701 ttgcaggttt atgaagttgg caccgccacc atgatggcat cgaaagccat taaaccggta
     5761 tatgacgtgt ttaaagaggc ggggattcaa ttcgatgagt cgcagtttgt gccgacggtt
     5821 tccggttact actccgacag caaaacgggc cacttactct cccagccatt caacagctcg
     5881 actcccgttc tctattacaa caaagacgcc ttcaagaaag cggggttaga cccggaacag
     5941 ccgccgaaaa cctggcagga tctggcggac tatgccgcga aactgaaagc ctccggcatg
     6001 aagtgcggct acgccagcgg ctggcagggc tggatccaac tggaaaatcg acaccttccc
     6061 ggtgatcgac gccgccacgt ccggcgggcc ggttcaggcc accacgacgc tgatttataa
     6121 gatctaccgc gaaggtttta ccggactgga tctggcttcg tctgccgcac agtcggtggt
     6181 gttgatgttc ctcgtcatcg tgctgacggt ggtgcagttc cgctatgttg aaagcaaggt
     6241 gcgttaccaa tgattgagaa ccgtccgtgg ctgacgatat tcagccatac catgctgatc
     6301 ctcgggatcg cggtgattct cttcccgctg tacgtggcgt ttgtcgcggc gacgctggat
     6361 aaacaggccg tctatgccgc gccgatgacg ctcatccccg gcacacatct gctggaaaat
     6421 atccacaaca tctgggttaa cggggtaggt acgaatagcg cgccgttctg gcggatgttg
     6481 cttaacagct ttgtgatggc gttcagcatt acgctcggca aaattaccgt ctcgatgctc
     6541 tcggcatttg ccattgtctg gtttcgtttt ccgctacgta acctcttctt ctggatgatt
     6601 tttatcaccc tgatgctgcc ggttgaagta cgtatcttcc cgacggtgga agtcatcgcc
     6661 aacctgaaga tgctcgacag ctacgccggt ttaacgctgc cgctgatggc ctcggcgacc
     6721 accacgtttc tgtttcgcca gttctttatg acgctgccgg atgaactggt ggaagcggcg
     6781 cggatcgacg gcgcatcgcc aatgcgcttc ttttgcgaca tcgtttttcc gctctccaaa
     6841 actaatctgg cggcgctgtt tgtgatcacc tttatttacg gctggaacca gtatttgtgg
     6901 ccgttgttga ttattaccga cgtggatctc ggcaccaccg tggcagggat caaagggatg
     6961 atcgccactg gcgaaggcac cacggaatgg aactcagtga tggcggcgat gttgttaacc
     7021 ctgatcccac cagtagtgat tgttttagtg atgcagcgtg ccttcgtgcg cggcctggtc
     7081 gatagtgaga aataagatgg caggactgaa attacaggca gtaaccaaaa gctgggatgg
     7141 caaaacccag gtcattaaac cgctgaccct tgatgtggct gatggcgaat ttatcgtgat
     7201 ggtcgggccg tctggctgcg ggaaatcgac gctgctgcgt atggttgccg ggctggagcg
     7261 ggtgaccgaa ggcgatatct ggatcaacga ccagcgcgtg actgaaatgg agccgaaaga
     7321 tcgcgggatt gcgatggtgt tccagaacta cgcgctttat ccgcatatga gcgtcgaaga
     7381 aaacatggcg tgggggctta aaattcgcgg catgggcaag cagcaaattg ccgagcgcgt
     7441 taaagaagcg gcgcgcattc tggagctgga cggtctgctc aaacgtcgcc cgcgtgagct
     7501 atccggcggt cagcgtcagc gtgtagcgat gggccgcgcg attgtgcgcg atccggcggt
     7561 gttcctgttt gatgagccgc tctctaacct cgatgccaag ctgcgcgtgc agatgcgtct
     7621 tgaactgcaa cagttgcacc gtcgcctgaa aacgacttca ctctacgtta ctcacgatca
     7681 ggttgaagcg atgacgctcg cccagcgagt aatggtgatg aacggcggcg ttgccgaaca
     7741 gattggcaca ccagttgaag tctacgaaaa gcccgccagc ctgtttgtag cgagttttat
     7801 cggcagtccg gcgatgaacc tgctgacagg ccgcgtgaat aacgaaggca cgcatttcga
     7861 actggacggc ggtattgagc tgccgctaaa cggtggctac cgtcagtatg ccgggcgtaa
     7921 aatgactctc ggcattcgcc cggaacatat tgtgctaagc tcgcaggcag aaggcggcgt
     7981 accgctggtg atggacacgc tggagatcct cggcgcagat aacctggcgc acggacgctg
     8041 gggcgagcag aagctggtgg tgcgactggc gcatcaggag cgcccgacgg caggcagcac
     8101 gctgtggctg catctggcgg aaaatcagct gcatcttttt gatggtgaaa caggacaacg
     8161 agtatgagta actggcctta tccccgcatc gtcgctcatc gtggcggcgg taagctggcc
     8221 ccggaaaaca ccctggcggc aatcgacgtc ggggcaaaat acggtcataa gatgatcgaa
     8281 tttgacgcga agttatcgaa agatggcgag atcttcctgc tccatgacga caatctcgaa
     8341 cgcaccagca acggctgggg cgtcgtgggt gaactgaact ggcaggattt actgcgcgtg
     8401 gatgcgggca gttggtacag caaagcgttt aaaggtgagc cgctgccgtt gctttcgcag
     8461 gtggcggaac gctgtcgcga acatgggatg atggcgaata tcgaaatcaa acccaccact
     8521 ggcactggac cattaacggg caaaatggtg gcgctggcgg cacgccaact gtgggccggt
     8581 atgacgccgc cgctgctgtc atcgtttgag attgatgctt tagaagctgc acagctggcg
     8641 gcaccggaac tgccgcgtgg tttattgctt gatgagtggc gcgacgactg gcgcgaactg
     8701 accgcgcggc tgggctgcgt ctctattcat ctcaatcata agttactcga taaagcgcga
     8761 gtgatgcagt tgaaagacgc cggattacgg attctggttt ataccgtcaa taaaccccag
     8821 cgcgcggcag agttgctgcg ctggggcgtg gattgcatct gtaccgatgc gattgacgtg
     8881 attggtccga actttacggc ccaatagttt tcaacggaat gtcaggctgc ggcgtgctgg
     8941 gctgattcag catgtcgccg tttctcctct ccggcagcat atgctgctga ttcaacgaac
     9001 tatccggatt acggttgctg tttaacatcc cgccgttggt gttgggcagc atttgctgcc
     9061 gggcgggatt tcgctccccc ggctgtgact gcaacacccg ctgagaatta ttgtttatct
     9121 ggttttctaa atgctgctgt tgcaactgag tttgcgtttt cagttgctga ttcagcatcc
     9181 ctttttgctg gatttgctga gtttgcatct gggtttgcat ccgctgctgg ctgggtatct
     9241 gataccccgg ctggttaggg ttgttcagag tattaatggg ctgtgcaaag ccgacaaacg
     9301 gcaggagtgc cgtaagaatc agaagtcgtt tcatcgcgta tcctcctctg aagatatcct
     9361 ttaagtttac tcgcttcccg acaaaacgat gattaattca gagttatata ccaggcttag
     9421 ctggggttgc cccttaatct ctggagaata acgatgataa aaccgacgtt tttacgccgg
     9481 gtagccattg ctgctctgct ctcaggaagt tgttttagcg ccgtcgccgc gcctcctgtt
     9541 tcttacggcg tggaggaaga tatcttccat ccggtacgcg cgaaacaggg gatggtggcg
     9601 tctgtcgatg ccactgccac ccaagtgggg gtagatattc taaaggaggg cgggaatgcc
     9661 gttgatgccg ccgtggcggt gggctacgcg ctggcggtaa cgcatccgca ggcagggaat
     9721 ctgggcggtg gtggttttat gctgatccgc tcgaaaaatg gcaataccac cgctatcgat
     9781 ttccgcgaga tggcacccgc taaagcgacc cgcgatatgt tccttgatga ccagggcaac
     9841 ccggacagca aaaaatcact cacttcgcat ctggcttccg gcacgcccgg tacggtcgct
     9901 ggtttctcgc tggcgttgga taagtacggc actatgccgt tgaacaaagt ggtgcagccc
     9961 gcgttcaaac tggcacgcga tggttttatc gttaacgatg ctttggcaga cgatctcaaa
    10021 acctacggta gcgaagtgtt gccgaatcac gaaaacagca aagctatctt ctggaaagag
    10081 ggcgaaccgc tgaaaaaggg cgacaagctg gtgcaggcga acctggcaaa gagcctggag
    10141 atgattgccg aaaacggccc ggacgaattc tataaaggca cgatagcgga acagattgcc
    10201 caggagatgc agaaaaacgg tggcttgatc actaaagaag atttagcggc gtataaagcg
    10261 gtcgaacgca caccgataag tggcgattat cgcgggtatc aggtttactc catgccaccg
    10321 ccatcctccg gcgggatcca tatcgtacaa atcctcaata ttctggaaaa cttcgatatg
    10381 aagaaatacg gctttggcag cgccgatgcg atgcaaatca tggcagaagc ggagaaatac
    10441 gcctacgccg accgctcgga atatcttggc gacccggatt ttgtcaaagt accgtggcag
    10501 gcgctgacca ataaagccta tgccaaatct attgccgatc aaattgatat caataaagcg
    10561 cagccatcca gcgaaattcg ccccggcaag cttgcgcctt atgagagtaa tcaaactacc
    10621 cattactcag tggtggataa agatggtaac gcggtggcgg tgacctatac gctgaacacc
    10681 accttcggta cgggcattgt cgcgggcgag agcggtattc tgcttaataa tcagatggat
    10741 gatttctccg ctaaaccggg cgtaccgaac gtttacgggc tggtgggcgg tgatgccaac
    10801 gccgtcgggc cgaataaacg tccgctgtcg tcaatgtcac caacgattgt ggtgaaagac
    10861 ggtaaaacct ggctggtcac cggtagccct ggcgggagcc ggatcatcac tacagtgctg
    10921 caaatggtgg tgaatagcat cgattatggc atgaacgtcg ccgaagcgac caatgcgccg
    10981 cgtttccacc atcagtggtt gccggacgag ctgcgtgtcg aaaaagggtt tagcccggat
    11041 acgctcaagc tgctggaagc aaaaggtcag aaagtggcgc tgaaagaggc gatgggcagt
    11101 acacaaagca ttatggttgg gccggacggt gagttgtacg gcgcatccga cccgcgctcg
    11161 gtggatgatt taacggcggg gtactaaggt tagcggccct cttcgtggga agagggctat
    11221 tttgtcaggg caagccgaag gtagcctttt ttatttcgta atcctgtaga tattcttcca
    11281 gcggctttgc cgttcccaga gaatgaattt caccactgtc tttatcaata ataaaaggtg
    11341 cgttaccagc taagcgcgcg gcctcatctc cagtttcgag aaattctcgt gcttcgaaac
    11401 agaaatacca gccctagcta aagcgtccat gtagagtaat gacgaccggg agatctgcat
    11461 catcaaggta atggttcgct ttcgcgaatg cgtcgtgata agtaatcata attataataa
    11521 atattattgt tgagtgttat attattattc ctgtgtgata cattgagcaa agacgcgttc
    11581 atcttcgtca aagatattat taaatgctgg ttttgaaaaa agttcatcgg ataagatata
    11641 gtcacgaaat aagtatctgc taatatttta tgatttcttt ttcagaacgt cgaacgggat
    11701 tttcttattt aagatatcag aatcttatga ttcgggtaaa attctaaata aatcaacatg
    11761 tcattaaaat catcatagcc attagctatt tcggctaaaa tagagactac atgtcttcgg
    11821 tccatctcac ttaaggagtg tagttccgtt gtaagttttt ccatagcttg cactgctaaa
    11881 tttcgaacaa ggaattttct gctggtaatc tctaaaaaga tggca
//
LOCUS       DADLFA010000126        11881 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000126 DADLFA010000000
VERSION     DADLFA010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11881
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.126"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(39..353)
                     /locus_tag="JQH65_002895"
                     /pseudo
     CDS             complement(39..353)
                     /locus_tag="JQH65_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
     gene            complement(468..875)
                     /locus_tag="JQH65_002896"
     CDS             complement(468..875)
                     /locus_tag="JQH65_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HAY6140254.1"
                     /translation="MIIMGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQS
                     GGLGAILSTWLSNQQSNQSVSGEQLESAPGTNAVSDLGQKLGVDTSTASSLLAEQLPK
                     IIDALSPQGEVSSQANNDLLSAGMELLKGKLFR"
     gene            complement(1106..3520)
                     /gene="gyrB"
                     /locus_tag="JQH65_002897"
     CDS             complement(1106..3520)
                     /gene="gyrB"
                     /locus_tag="JQH65_002897"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAY6140255.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADLLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(3549..4622)
                     /gene="recF"
                     /locus_tag="JQH65_002898"
     CDS             complement(3549..4622)
                     /gene="recF"
                     /locus_tag="JQH65_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAY6140256.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWACFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLVEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(4622..5722)
                     /gene="dnaN"
                     /locus_tag="JQH65_002899"
     CDS             complement(4622..5722)
                     /gene="dnaN"
                     /locus_tag="JQH65_002899"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAY6140257.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(5727..7130)
                     /gene="dnaA"
                     /locus_tag="JQH65_002900"
     CDS             complement(5727..7130)
                     /gene="dnaA"
                     /locus_tag="JQH65_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAY6140258.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            7427..7507
                     /locus_tag="JQH65_002901"
     CDS             7427..7507
                     /locus_tag="JQH65_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6140259.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            7737..7877
                     /gene="rpmH"
                     /locus_tag="JQH65_002902"
     CDS             7737..7877
                     /gene="rpmH"
                     /locus_tag="JQH65_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAY6140260.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            7894..8253
                     /gene="rnpA"
                     /locus_tag="JQH65_002903"
     CDS             7894..8253
                     /gene="rnpA"
                     /locus_tag="JQH65_002903"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAY6140261.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            8217..8474
                     /gene="yidD"
                     /locus_tag="JQH65_002904"
     CDS             8217..8474
                     /gene="yidD"
                     /locus_tag="JQH65_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAY6140262.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            8477..10123
                     /gene="yidC"
                     /locus_tag="JQH65_002905"
     CDS             8477..10123
                     /gene="yidC"
                     /locus_tag="JQH65_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAY6140263.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKTQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            10229..11593
                     /gene="mnmE"
                     /locus_tag="JQH65_002906"
     CDS             10229..11593
                     /gene="mnmE"
                     /locus_tag="JQH65_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAY6140264.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            11836..>11881
                     /locus_tag="JQH65_002907"
     CDS             11836..>11881
                     /locus_tag="JQH65_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HAY6140265.1"
                     /translation="MNILHICVTSKWFNI"
BASE COUNT         2716 a         3252 c         3070 g         2843 t
ORIGIN      
        1 ggacaggggt atcgcttagt ttttcaattt cctatacgtg aaccgacttc gcgagagagt
       61 ttttccagga aacgatagtc gtcataactg agagcagttt gcgccacggt gctaccatca
      121 gcggccttct gtaccagcgc gccgttataa gtaatgcagt agtcgcccgg ctgttccata
      181 tgcagctctt tcaggtagtt gtgaacgcct gcataagggc gacccgtcgt tagcacgaca
      241 ttcacgccac gggcgcgagc tgcggcaatc gcatttttaa cggcgggtga aatggtgtga
      301 tcgggcagca gaagggtgcc atccatatcg atagcaatga gtttgatagc catgagttcc
      361 ccaggtagat tagttcctga cccatgctaa cgcgattccg ctcaaaaatc agtacaacac
      421 ccgagggaaa agggggatgc aacgcgggtt gcatgctccc catttgctta gcggaagagt
      481 ttccctttca gcagttccat acctgcggaa agcagatcgt tattggcttg tgaagacact
      541 tcaccttgcg gtgagagcgc atcaataatc ttcggcaatt gttctgccag taaactggaa
      601 gccgtactgg tatccacgcc aagtttttgc ccgagatcgg acaccgcatt tgtgccgggc
      661 gccgattcca gttgctcgcc gctaactgat tgattgctct gctgattact caaccaggtt
      721 gagagaatgg cccctaagcc gccactttgc agtttttcca gcagcacctg aatgccgccc
      781 tgctcctcaa cccaacttaa aatagcctga tatttccccg catcgccttt cagaaaggca
      841 ccgacaactt catcaaaaag ccccatgata atcacctgta aagcgttacg tgttgaccca
      901 aaaagtatag atctgcggga tgataattgc ggattgcaga aataaaaagg gcggagatga
      961 tctccgccct ttttttatag cttcttgccg gatgcggcgt taacgtctta cctggtctac
     1021 aaaatcatta aaattcaata cattgcaaga ttttcgtagg cctgataagc gtagcgcatc
     1081 aggcacgctc gcatggttaa cgccattaaa tatcaatatt cgccgctttc agggcgttct
     1141 cttcaataaa cgcacgacgc ggttctacgg cgtcgcccat cagcgtggtg aacaacaggt
     1201 cagcggcaat cgcatcctta acggtaacgc gcagcatacg acgactttcc gggtccatgg
     1261 tggtttccca cagctgttcc gggttcatct cgcccagacc tttataacgc tggatggaga
     1321 gaccgcgacg agactctttc accagccagt ccagcgcctg ctcgaagctg gctaccggct
     1381 gacgacgctc gccacgttcg ataaatgcat cttcttccag caagccacgc agtttctcac
     1441 ccagcgtgca gatacgacga tattcgccac cagtgataaa ttcgtgatcc agcggatagt
     1501 cagtatccac accgtgggta cgcacgcgaa caatcggctc gaacaggttt tgttcggcat
     1561 tggtgtggac atcaaacttc cactggctgc cgtgctgttc tttgtcgttc agttcgctga
     1621 ccagcgcgtt cacccagcgg gtaacggtct gctcatcaga gaggtcggct tccgtcagcg
     1681 tcggctgata gataagctct ttcagcattg ctttcggata acgacgctcc atgcggttga
     1741 tcattttctg cgtcgcgttg tactcagaca ccagtttctc taacgcttcg ccagccaatg
     1801 ccggtgcgct ggcgttggtg tgcagcgttg cgccgtccag cgcgatagag atctggtact
     1861 gatccatcgc ttcgtcgtct ttaatgtact gttcctgctt gcctttcttc actttgtaca
     1921 gcggcggctg agcgatgtag acgtgaccgc gttcaacgat ttccggcatc tgacgataga
     1981 agaaggtcaa cagcagcgta cgaatgtgcg agccgtcgac gtccgcatcg gtcatgatga
     2041 tgatgctgtg ataacgcagt ttgtccgggt tgtactcgtc acgaccgata ccacagccaa
     2101 gcgcggtgat aagcgtcgcc acttcctgag aagagagcat cttatcgaag cgcgctttct
     2161 caacgttgag gattttaccc ttcagcggca gaatcgcctg gttcttgcgg ttacgcccct
     2221 gcttcgcaga gccgcccgcg gagtcccctt ccaccaggta cagttcggaa agcgccggat
     2281 cgcgttcctg gcagtctgcc agtttgcccg gcaggcccgc taagtcgagc gcacctttac
     2341 ggcgggtcat ttcacgcgcg cgacgcgccg cttcacgggc acgggcagca tcgataattt
     2401 tgccaaccac gattttcgcg tcggttgggt tttccagcag gtattctgcc agcagttcgt
     2461 tcatctgctg ttcaaccgcc gatttcacct cagaagaaac cagtttgtct ttggtctggg
     2521 aggagaattt cgggtccggc acttttacgg aaacgaccgc aatcaggcct tcacgcgcat
     2581 cgtcaccggt ggcgctgact ttggcttttt tgctgtagcc ttctttgtcc atgtaggcgt
     2641 tcagggtacg ggtcatcgcc gcacggaagc ctgccaggtg agtaccgccg tcacgctgcg
     2701 gaatgttgtt ggtaaagcag tagatgtttt cctggaagcc atcgttccac tgcaacgcca
     2761 cttcgacgcc aataccgtct ttttcggtgg agaagtagaa gatattcgga tggatcggcg
     2821 ttttgttctt gttcagatat tcaacgaacg ccttgatgcc gccttcatag tggaagtggt
     2881 cttctttgcc gtcacgcttg tcgcgcagac gaatggaaac gccggagttg aggaacgaca
     2941 actcacgcag acgtttcgcc agaatttcat attcgaactc ggtcacattg gtgaaggttt
     3001 cgaggctggg ccagaaacgc accatggtgc cggttttttc agtctcgccg gtaaccgcca
     3061 gcggagcctg cggtacaccg tgttcgtaga tctgacggtg aattttaccc tcgcgctgga
     3121 taaccagctc cagtttttgc gacagggcgt ttactaccga aacaccaacg ccgtgcagac
     3181 cgccggacac tttataggag ttatcgtcaa atttaccgcc tgcgtgcaga acggtcatga
     3241 tcacttccgc cgccgatacg ccctcttccg ggtgaatacc ggtcggaatg ccgcgcccgt
     3301 catcctgtac agagacagag ttatcggcgt gaatggtgac gataatttct ttacagtgac
     3361 ccgcgagcgc ttcgtcgata gcgttatcta ccacctcgaa taccatgtgg tgcagaccgg
     3421 tgccgtcatc cgtgtcgccg atatacatac ccgggcgctt acgcaccgca tccagccctt
     3481 tcaggacttt gatactggag gagtcataag aattcgacat caacgtttct cgctcattta
     3541 tacttgggtt aatccgttat tttacccttt tccacggtaa acatcttcga attttcgtcc
     3601 gacatgtcta taacgtgttc agcactgatc gcgctgacaa agacctgtga ttgcgtcgct
     3661 tttaagcggc tggcaagcag cccgcgacgc tcatcatcaa gctcagaggc aaaatcatct
     3721 atcaggtaga gacaccgccg cccgctttca cgggtgagga actctccttg cgccagacgt
     3781 aaggcgcaca tcaacagctt aagctgccca cgcgataagg tatcttccac cggcgcaccg
     3841 tcggcgcgaa tgcgtaagtc cgctttatgc gggccatgcg cggtgtaggt tagctggcga
     3901 tcgcgttcaa aattacgttc cagcacctca gcatattctg tctctttctc ccagccgcgc
     3961 tggaaagaga aagtcagaga aaactcaggg agaaattgct tacaagtatc ggccatatca
     4021 gccgcgatac cggcgctata ctccgcgcgc caggtgctga tttgctccac cagcgggatc
     4081 agctctttat cccacgggcg tagctgttcg taacgtgtca cctggcgcag cgccgcattg
     4141 cgctgcttga gcaatcgctt gagattgctc caggcggtga aaaatccggg ttcgttgtga
     4201 aagcatgccc agtcgagaaa tgctcttctg tatttggggc cgccgttgag taaagtaaac
     4261 ccttctggcg ttatcagctg catcggcatc aggtgcgcca gctccgcgac tttatgcccg
     4321 tcagtaccgt cgatgcggac tttgctgtcg ccttgtttgt ctttggttaa gccaatcgcc
     4381 gtctcgcgct cttcgccctg taatcgccca tggagaacaa atgcctcctg ctcatggcga
     4441 atgacgcgac caatctgcaa actgcgaaac gcccgaccat ggccgagcgt atagatggct
     4501 tccagcacac tggttttgcc actgccgttg gcacctacca gaaagttaaa gccgggagat
     4561 aaggcgagat ccgcggtttc gatattgcgg aaatcgcgga tcaacaagcg ggtgagggac
     4621 attacagtct cattggcatg acaacataag ccgcgctctg gctggccgca tcttcaatct
     4681 gcacgctgga aaccgaatcg gtcagcatca tgcggacgtt ttcgcatttc agcgcgttca
     4741 gaacatccag cacatagctg acgttgaagc cgatttccat ctccgcaccg ctataggtaa
     4801 cgtcgaggat ctcttccgct tcttcctgtt ccgggttgtt ggcagtgatt ttcagctggt
     4861 tttcgctgac ataaagacgt acgccgcgga atttctcgtt agagagaatt gccgcacggg
     4921 caaacgcctg cttgagcaga tcgcagccag cttccagatg tttgtccggg ttcttcggta
     4981 gaacgcggcg gtaatccggg aagcgaccat ccaccagttt ggaggtgaag ataaagtcgc
     5041 caacgtgggc acgaatgtta ttgctgccaa tctgcacgcg cagcggattg tcgccgccgt
     5101 cgagcatacg catcagttca atcacgcctt tacgcggcac gatcaccgaa tggcttggca
     5161 aagattgacc aattggcatt gaacagactg ccagacggtg accatcggtc gctacggtgc
     5221 gcagttcttc gccttcggtt tcaaacagca taccgtttaa gtaatagcga acatcctgat
     5281 gcgccatcga aaactgggtc gcttcaatca gacgcttcat cgtcgcctgc ggcagggtaa
     5341 attcgacttc gctctgccag tcatcgaggt tcgggaaatc cgccgctggc agggtagaca
     5401 gcgaaaaacg gctacgcccg gagcgcacca gcatccgttc accttccagc tgcacggcaa
     5461 tttccgcgcc ttcaggcaga ccacggcaga tatcaaagaa tttgcgcgcc ggaacggtcg
     5521 ttgctcctgg ctcgtgtggc tgaaccagcg caacacgtgc caccatttcc atctcgagat
     5581 cagtaccggt cagcgacaac gtaccgtcag caacctgtaa cagcagatta ccgagaatcg
     5641 gtagcgtagg acgaccacct aacggaccgc tcacctgttg tagcggtttt aataaatgct
     5701 cacgttctac ggtaaatttc ataggtttac gatgacaatg ttctgattaa atttgaaaaa
     5761 tcttctttga tatcgtggct ctcttcacgc aactgctcga tcttacggca ggcatgaagc
     5821 accgtcgtgt ggtcacgacc accaaacgca tcgccaatct ccggcagact gtggttagtc
     5881 agctctttcg ccagcgccat cgccatctgg cgcggacgcg ccaccgagcg ggatcgacgc
     5941 ttggaaagga gatccgcgac tttgatcttg tagtactccg ccaccgtctt ctgaatattg
     6001 tcgatggtga ccagtttttc ctgcaatgcc agcaagtcgc gcagcgcctc acgcacgaag
     6061 tcgatggtga tcgcccgtcc ggtaaagttg gcattggcaa tgacgcggtt cagcgcccct
     6121 tccagctcac gtacgttaga tcgtagacgc ttggcgataa agaacgccac ttcgcccggc
     6181 aaacgaatgt cgttttcgtc ggcctttttc atcaggatcg ccacacgggt ttccagctct
     6241 ggcggttcga tcgccacagt cagtccccaa ccgaagcggg atttcaaacg atcctcaacg
     6301 ccgttgatct ctttcggata gcgatccgag gtgagaatga tctgttgatt accttccagc
     6361 agggcgttga aggtatggaa aaactcttcc tgagatcgtt ctttattagc aaaaaactga
     6421 atatcgtcga tcagcagtgc atctacggaa cggtagtagc gtttaaactc ttcgatcgcg
     6481 ttgttttgca gggctttaac catgtcctga acaaagcgct cggagtgcat ataaaccact
     6541 ttggcattcg gcttgcgcgc cataatgccg ttacccaccg catgcagcag gtgagtttta
     6601 cccagacccg tgccgccata aaggaacaac gggttatagg caccgccagg gttatccgcc
     6661 acctggcgag ccgccgcgcg cgccagttgg ttagatttac cttcaacgaa gttatcaaac
     6721 gtgtgtttga cgtttacgtt agaacgatag gtcggttctg ccggggccgg gacgttatcc
     6781 caacctgagc gcgtagaagg cgcagcacgt tgcggctgcg tttgcgccac ctgtgcaggg
     6841 gccgcgacgt tgctcgtcac tgccgcttgt ggcgtttgcg tcaccggttt ggtgccgact
     6901 tcaaaacgca gctgtggggc atccgctccg cagaaactgg ttagcagtcc attgatatta
     6961 ttaaggtact tgtcccgtac ccaatcgagg acaaaacggt ttggcgcgta cagggccagc
     7021 gtgttatcgc tcagttccgc ctgcaatggg cgtatccaca tactgaattc tgtggctggt
     7081 aactcatcct gcaatcgggc aagacactgc tgccaaagcg aaagtgacac ggcggactcc
     7141 actcgaacaa aagtcgataa tgactaaggc tgaaacattc atgattgttg acgtacgtcg
     7201 aaaagaccct gcttgagggt gacgcacgaa ccgctgtctg cggttatatg cccgatcaag
     7261 atcctgcaaa acgatcggga ccgcggatca tagcctaaac tgcgcaagag atcttctgtt
     7321 tctcacagat tttcccgatt tatccacagg actttccaga actcgctaag tgtaaacgat
     7381 cctgccgcga ggcgggcacg atttacgccg catattgaaa aatttaatga ccatagacaa
     7441 aaattggctt aatcgatcta ataaagatcc aggacgatcc ttgcgcttta cccatcagcc
     7501 cgtataatcc tccacccggc gcgccatgct ggtttccact ggtgtgaggt cgtacatttt
     7561 ccctgcgaaa aggtgcggaa aagcgcggta aataaggaaa gagaattgac tccggagtgt
     7621 acaattatta caatccggcc tctttaatca cccatggctt cggtgtccat cgtttcattt
     7681 ttcggcggat atccaataaa gccattgaat ttattcaagt ttaggtagaa atcgccatga
     7741 aacgcacttt tcaaccgtct gtactgaagc gcaaccgttc tcacggcttc cgtgctcgta
     7801 tggctactaa aaatggtcgt caggttctgg cacgtcgtcg tgctaaaggc cgcgctcgtc
     7861 tgaccgtttc taagtaataa agctaacccc tgagtggtta agctcgcatt tcccagggag
     7921 ttacgcttgt taactcccag tcaattcaca ttcgtcttcc agcagccaca acgggctggc
     7981 acgccgcaaa ttaccattct cggccgcctg aattcgctgg ggcatccccg tatcggtctt
     8041 acagtcgcca agaaaaacgt tcgacgcgcc catgaacgca atcggattaa acgtctgacg
     8101 cgtgaaagct tccgtctgcg ccaacatgaa ctcccggcta tggatttcgt ggtggtggcg
     8161 aaaaaagggg ttgccgacct cgataaccgt gctctctcgg aagcgttgga aaaattatgg
     8221 cgccgccact gtcgcctggc tcgcgggtcc tgatagccct cattcgggtc tatcaacgcc
     8281 tgattagtcc gctactcggg ccgcattgtc gtttcactcc aacctgttca agctacggaa
     8341 ttgaggcatt gcgcaggttt ggagtgataa aaggcagttg gttgacggtg aaacgcgtat
     8401 taaaatgcca ccctttacac cctggtggtg acgatcccgt cccgcccgga ccatttgata
     8461 ccagagaaca ctaacgatgg attcgcaacg caatctttta gtcatcgctt tgctgttcgt
     8521 gtctttcatg atctggcaag cctgggagca ggataaaaac ccgcaacctc aggcccaaca
     8581 gaccacgcag acaacgacca ccgcagcggg tagcgccgcc gaccagggcg taccggccag
     8641 tggccagggg aaactgatct cggttaagac cgacgtgctt gatctgacca tcaacacccg
     8701 tggtggtgat gttgagcaag ctctgctgcc tgcttacccg aaagagctga actctaccca
     8761 gccgttccag ctgctggaaa cttcaccgca gtttatttat caggcacaga gcggtctgac
     8821 cggtcgtgat ggcccggata acccggctaa cggcccgcgt ccgctgtata acgttgaaaa
     8881 agacgcttat gtgctggctg aaggtcaaaa cgaactgcag gtgccgatga cgtataccga
     8941 cgcggcaggc aacacgttta ccaaaacgtt tgtcctgaaa cgtggtgatt acgctgtcaa
     9001 cgtcaactac aacgtgcaga acgctggcga gaaaccgctg gaaatctcca cctttggtca
     9061 gttgaagcaa tccatcactc tgccaccgca tctcgatacc ggaagcagca acttcgcact
     9121 gcacaccttc cgcggcgcgg cgtactccac gcctgacgag aagtacgaga aatacaagtt
     9181 cgataccatt gccgataacg aaaacctgaa catctcttcg aaaggtggtt gggtggcaat
     9241 gctgcaacag tatttcgcga cggcgtggat cccgcataac gacggtacca acaacttcta
     9301 taccgctaat ctgggtaacg gcatcgccgc tatcggctat aaatctcagc cggtactggt
     9361 tcagcctggt cagactggcg cgatgaacag caccctgtgg gttggcccgg aaatccagga
     9421 caaaatggca gctgttgctc cgcacctgga tctgaccgtt gattacggtt ggttgtggtt
     9481 catctctcag ccgctgttca aactgctgaa atggatccat agctttgtgg gtaactgggg
     9541 cttctccatt atcatcatca cctttatcgt tcgtggcatc atgtacccgc tgaccaaaac
     9601 gcagtacacc tccatggcga agatgcgtat gctgcagccg aagattcagg caatgcgtga
     9661 gcgtctgggc gatgacaaac agcgtatcag ccaggaaatg atggcgctgt acaaagctga
     9721 gaaggttaac ccgctgggcg gctgcttccc gctgctgatc cagatgccaa tcttcctggc
     9781 gttgtactac atgctgatgg gttccgttga actgcgtcag gcaccgtttg cactgtggat
     9841 ccacgacctg tcggcacagg acccgtacta catcctgccg atcctgatgg gcgtaacgat
     9901 gttcttcatt cagaagatgt cgccgaccac agtgaccgac ccgatgcagc agaagatcat
     9961 gacctttatg ccggtcatct tcaccgtgtt cttcctgtgg ttcccgtcag gtctggtgct
    10021 gtactatatc gtcagcaacc tggtaaccat tattcagcag cagctgattt accgtggtct
    10081 ggaaaaacgt ggcctgcata gccgcgagaa gaaaaaatcc tgattcggtg agttttcgct
    10141 aaaataaggg cggtcagttg accgcctttt ttcttttcgt agggcggata agcaccgcgc
    10201 atccgccaca caaagcaaca ggaacatcat gagcgataat gacactatcg tagcccaggc
    10261 cacgcctccg ggacgtggcg gcgttggcat cctgcgcatc tccggcttca aagcccgtga
    10321 agttgccgaa accgtgctgg gtaaactgcc taagccgcgc tacgccgatt atcttccgtt
    10381 taaagacgcc gacggcagcg tgctcgatca ggggattgcg ctatggttcc ctggcccgaa
    10441 ctcgttcacc ggcgaagatg tgctggaact gcaaggtcat ggcggtccgg tgatcctcga
    10501 cctgctgtta aaacgcattc tgaccattcc cggcctgcgg attgctcgcc ctggtgagtt
    10561 ttccgaacgc gcgtttctta acgataaact tgacttagcc caggccgagg cgattgccga
    10621 tcttatcgac gccagttcgg aacaggcggc ccgttcggca cttaactcgc tgcaaggcgc
    10681 attctccgca cgggttaatc atctggtaga agccctcacc cacttgcgca tttacgtcga
    10741 agcggcaatt gatttccccg atgaagagat cgatttcctc tccgacggaa aaattgaagc
    10801 ccagctcaat gacgttattg ccgatcttga tgcagtgcgt gctgaagcac gtcagggtag
    10861 tttgttgcgc gaagggatga aagtggtgat tgccggacgt cctaacgccg gtaaatcgag
    10921 cctgttaaac gcgctggcgg ggcgtgaagc ggcaatcgta accgatatcg ccggaactac
    10981 gcgtgacgtg ctgcgtgagc atatccacat tgacggaatg ccgctgcata tcatcgatac
    11041 cgccgggcta cgtgaagcca gtgacgaagt agaacgtatt ggtatcgagc gcgcgtggca
    11101 ggaaattgaa caggccgacc gcgtgctgtt tatggtcgat ggcaccacaa cagacgccgt
    11161 ggatccggca gagatctggc cggaatttat tgcccgtctg ccagcgaaac tgccgatcac
    11221 cgtggtgcgc aataaagccg atatcaccgg cgaaacgctg ggaatgagtg aagtgaacgg
    11281 tcacgcgtta attcgtctct cggcaaggac tggtgaaggc gtggacgtgc tgcgtaacca
    11341 tctcaaacag agcatgggct ttgacaccaa catggaaggc ggcttcctgg cgcgtcgtcg
    11401 ccacctacag gcgctggaac aggcagcgga acatctacaa cagggcaaag cgcaactgtt
    11461 gggagcctgg gcaggtgaac tgctggcgga agagttgcgt ctggcacagc agaacttaag
    11521 cgaaatcacc ggggaattta cttcagacga cctgctgggg cggattttct ccagcttctg
    11581 tattggtaag taaccgcgct tacgaagccg tattctgact gtcagatgcg gcttcgcttc
    11641 attgttagca ctcctgttat tcctcaaccc tttttttaaa cattaaaatt cttacgtaat
    11701 ttataatctt aaaaaaagca tttaatattg ctccccgaac gattgtgatt cgattcacat
    11761 ttaaacaatt ttagaataga caaaattctt gagtattata ctgtagcctc gtgtctttcg
    11821 aggaataagt gcattatgaa tatcttacat atatgtgtga cctcaaaatg gttcaatatc
    11881 g
//
LOCUS       DADLFA010000127        11825 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000127 DADLFA010000000
VERSION     DADLFA010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11825
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.127"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(37..273)
                     /locus_tag="JQH65_002908"
     CDS             complement(37..273)
                     /locus_tag="JQH65_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HAY6140266.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
     gene            414..1202
                     /gene="fhuF"
                     /locus_tag="JQH65_002909"
     CDS             414..1202
                     /gene="fhuF"
                     /locus_tag="JQH65_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HAY6140267.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(1240..1916)
                     /gene="bglJ"
                     /locus_tag="JQH65_002910"
                     /pseudo
     CDS             complement(1240..1916)
                     /gene="bglJ"
                     /locus_tag="JQH65_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
     gene            complement(1874..2599)
                     /locus_tag="JQH65_002911"
     CDS             complement(1874..2599)
                     /locus_tag="JQH65_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY6140268.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDF
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMNETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIVSLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            3218..3988
                     /locus_tag="JQH65_002912"
     CDS             3218..3988
                     /locus_tag="JQH65_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HAY6140269.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAIITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            3979..4452
                     /locus_tag="JQH65_002913"
     CDS             3979..4452
                     /locus_tag="JQH65_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAY6140270.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTRGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            4560..5099
                     /gene="dnaT"
                     /locus_tag="JQH65_002914"
     CDS             4560..5099
                     /gene="dnaT"
                     /locus_tag="JQH65_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HAY6140271.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            5102..5839
                     /gene="dnaC"
                     /locus_tag="JQH65_002915"
     CDS             5102..5839
                     /gene="dnaC"
                     /locus_tag="JQH65_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001731874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HAY6140272.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            5888..6382
                     /locus_tag="JQH65_002916"
     CDS             5888..6382
                     /locus_tag="JQH65_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HAY6140273.1"
                     /translation="MMETVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            6636..8927
                     /gene="opgB"
                     /locus_tag="JQH65_002917"
     CDS             6636..8927
                     /gene="opgB"
                     /locus_tag="JQH65_002917"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HAY6140274.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRHRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(9066..10088)
                     /locus_tag="JQH65_002918"
     CDS             complement(9066..10088)
                     /locus_tag="JQH65_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAY6140275.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVRLGKNIADLKNGLQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            10227..11141
                     /locus_tag="JQH65_002919"
     CDS             10227..11141
                     /locus_tag="JQH65_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY6140276.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTLINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            11356..>11825
                     /locus_tag="JQH65_002920"
     CDS             11356..>11825
                     /locus_tag="JQH65_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY6140277.1"
                     /translation="MEKENITIDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHNFTQFVLVRIGMGIGEAPMNPCGVKVIN"
BASE COUNT         3070 a         3014 c         2767 g         2974 t
ORIGIN      
        1 tatctttact tcctttcttg tttcttcctt gatttttcat ccctgattca aattcattat
       61 catcagcatg atggcagcaa taagcgccag gcacgatggt agcaaaacgt aatgccgtaa
      121 ctgtttgtga tacagcatca ccggcgtcaa tactaatccc atcagaatga caatccgcca
      181 ctgatccggc gataaatccg catacatcag ccctgcaatc agcaatcccg gcagcaacac
      241 tccccagcca ctgcccagcg tacgttgcaa catggtttca tctctttcat tgataatgat
      301 aactaatatc atatgataat ttttatcatt tgcaagccag agaaatccct tgctatcggg
      361 taaacctatc gctaggatta gcaatcatta tcatttagat tactatcccg attatggcct
      421 atcgttccgc accgctctat gaagatatca tctggcgaac gcatctccag ccgcaggatg
      481 ctgggcttgc acaagccgta cgtgcgacga tcgccaaaca tcgtgaacat ttgctcgagt
      541 ttatccgcct ggatgaaccc gctccgctta acgccatgac gctggcgcaa tggtcatcac
      601 cgaatgcgct aagttctcta ctcgcggtct attccgatca tatctatcgc aaccaaccaa
      661 cgatgatccg cgagaacaaa ccgctgatct cactatgggc acaatggtat atcggcctga
      721 tggtgccacc attaatgctg gcgctactga cgcaggaaaa ggcattagat gtgtcgccgg
      781 aacatttcca tgctgagttt cacgaaaccg gacgcgtcgc ctgtttctgg gtcgatgtgt
      841 gtgaagataa aaacgcaaca ccacattcgc cgcagcagcg aatggaaacg ttaatcagcc
      901 aggcgctggt tccggttgtg caggcactag aagcgaccgg agagatcaac ggcaaactta
      961 tctggagtaa taccggttat ttgatcaact ggtatctcac tgagatgaag caactgctcg
     1021 gcgaggcgac ggttgaatcg ctgcgccatg ccctcttttt tgagaaaacg ctcacgaacg
     1081 gtgaagataa tccactctgg cgtaccgtcg tgctgcgcga tggcctgctg gtgcgccgaa
     1141 cctgctgcca gcgttatcgc ttaccggatg tgcagcaatg tggcgattgt acgctgaaat
     1201 gaaagcactg ccggggaagt aaacccggca tcatgcggat taatagggat gcaacacatt
     1261 acttgcttcg caatgccgca tacataacag aatatctgcg gcctccaaca cccctgcatc
     1321 agaactgacg cccagtttcg acatcacatt aaatttatgt gcacggatcg ttttgatatt
     1381 gcgtttaagc tgctcggcaa tttgtgtcat tgagtagcca cgcgacataa agcgcaatat
     1441 ttctctctcc gtcgggctta gcgtccggct ttggttaatg taccactgat tgttcagtcg
     1501 gtcggtcgcc tgacgtacac catttaatga caaaaagagt tcctgatgaa aaatctccag
     1561 cgttgacgct ttacttaata caccgtccag cggtgatggc gacaatgaac caatcagccg
     1621 agcttcaata tcatcatccg caataactaa acgccgggta cgcggaaact taatcgccag
     1681 ttcagtcagg caagataatc cctctctgcg ctcacttctc atggcagaaa aagaaaaatg
     1741 accgccgcaa atgagatccg cgacatggca tcctgaaatg actcctgact ggtataggta
     1801 tggagcttat aagggttacc cgcaaacttt ctgaataaac tctcaatacc gatactgctc
     1861 atgacgcatt tttctatgag tgcgacattt ctcttcttaa ttcggctgtg ttccattctg
     1921 cggcatctcc ataaacagaa ggcaagttaa gttctcttgc accctgcgcg ctatttatcc
     1981 atgcatacat ttcagcgttg ctgtgaattg ccagtcgacg catcgcactg tttttttggg
     2041 cgctgatagt tttattactt ttcttaagca acgaaactat ctggttgatg ccccatcctt
     2101 tacctaatag ccgcagtacc ttgcgctctg aaagagtgag aatgacggag cgatagctaa
     2161 aatcatgagt ttcattcatt gccggagagg tcagcatggc gctgatacgc tctgcgtgcg
     2221 tattgccgga acgtacggtc ttgaccagat tctcaatggg ttcaacatca gacaataacg
     2281 tcgccgtagg gcacatgagc aattctactg cctgggaata ccaggagcgt gacaccatga
     2341 aaacccagtg aatttcccga tattgtgaga ttaaagaata ataatgttca caaacagaac
     2401 gtggatcttc actttcaccg gctaaatcag caatgactaa tccggaacga cgtaattgta
     2461 ataaggtaaa gtcctccgca gaggcgctgg atattatttc atattcaggg aagtgagtcc
     2521 tcatgacctc ttttaaccct gcttgcataa caggtatttt actgataaca attccatttt
     2581 tgcagcatcc tggcaacata tttcctccac atccactgtc taaaaatcat gatggtttat
     2641 tcaggatatt cattccttaa atgtttccag caaattaata gattaagcga ataaattatc
     2701 cttagcattc actttcatat cagcatactc ctgctgcaca acaactccac tgcatgtgtc
     2761 agaaggttag catcaaatga taacctggtg aaatagcctg gagacattta aaatatctac
     2821 attaagttgt taggaatact taatgaaaaa cagaaatcac attcatttaa tttataatta
     2881 aatgatatcc cttcccgcag aatgaaaagt cacacaacca atatttattc aatgaaatct
     2941 gatggtttta acttgttaac attaaaaaac gtgcccggtt atctattatc ctgattcgta
     3001 aaggccaaac aataaattca ttatagtgat aatattcatg tgatagtttg tttgcggcga
     3061 gagataattc gctttttatc accgagcttg tcccgataac caggattaat cagcgcaatt
     3121 ttgatcttaa cctaccgcaa aacaaccgtg cttcatgata caaatggatt ctcattatct
     3181 gaacaatacg cagcacgtct atgacaaagg gcgagttatg caaactgagc aacagcgagc
     3241 cgtaacacgg ctttgtatcc agtgtggatt atttcttttg caacatggtg cggaaagcgc
     3301 gttggttgat gagctttcct cacgactggg tcgggcgttg gggatggaca gcgtcgaaag
     3361 ttctatctct tcgaacgcca tagtgctgac aaccattaaa gatgggcaat gcctgacatc
     3421 gacacgtaaa aatcacgatc gcggcattaa tatgcatgtg gtgactgaag tccagcacat
     3481 tgtgattctg gcggagcacc atctgctgga ttacaaaggc gtagagaaac gatttagcca
     3541 aattcagcca ttacgttacc caagatggct ggtagcctta atggttggcc tttcttgcgc
     3601 ctgtttctgc aaactcaata aaggtggctg ggatggtgcc atcatcacct tctttgccag
     3661 tacggccgcg atgtatatcc gccagctgct ggcacaacgt catcttcatc cgcagatcaa
     3721 cttttgcctc accgctttcg ccgccaccac catttccgga ttgcttttgc aacttcccac
     3781 tttcagcaat acacccacta tcgcgatggc cgccagcgtt ctgctgctgg tgccgggatt
     3841 tccgttgatt aatgccgtcg ccgatatgtt taaaggccac atcaataccg gactggcacg
     3901 ctgggcgatc gccagtctgc tgacactggc tacctgcgtc ggcgtggtga tggcactgac
     3961 gatttggggg ctacgcggat gggtgtgatc gagtttctgt ttgcattggc gcaggatatg
     4021 atcctcgccg ccattccagc ggtcggcttt gcgatggtgt tcaacgttcc cgttcgggca
     4081 ttacgctggt gtgcgctgct tggcgcgatt ggtcatggtt cacgaatgat cttaatgacc
     4141 agagggctga atattgagtg gtcaaccttt atggcctcca tgctggtcgg caccattggt
     4201 attcaatggt cgcgctggta tctggcgcat ccgaaagtgt ttaccgtggc ggctgttatc
     4261 cctatgttcc cgggcatatc ggcttatacc gcaatgattt cggcggtaaa aatcagccag
     4321 ttaggttaca gcgaaccgtt gatgattacc ctgttaacca actttcttac agcttcatcg
     4381 attgttggtg cgttatccat cggtctttcc attcctggat tatggttgta ccgcaagcgc
     4441 cctcgcgtat aaaattgcct cgcttaaagc acacggatga gagacagcct cctctcctcc
     4501 gtgtgttact ataaaagtta tctcccttct cgttcatcgt tccatatttg agaaacagta
     4561 tgtcttccag agttttgacc ccggacgtcg ttggtattga cgccctggta cacgatcacc
     4621 aaaccgttct ggcaaaagct gaaggcggtg tggttgccgt atttgctaac aatgccccgg
     4681 cgttttatgc cgtcacgcct gcacgcctgg ctgaactgct ggcgctggaa gaaaagctgg
     4741 cgcgtccggg aagcgatgtc gctctggacg atcaactcta tcaggaaccg caagccgctc
     4801 ccgttgctgt acccatgggg aaattcgcca tgtatccgga ctggcaaccc gatgccgatt
     4861 ttatccgcct ggcggcgcta tggggtgtgg cactaagaga gccggtgacc accgaagaac
     4921 tggcctcatt cattgcctac tggcaggcgg aaggtaaagt ctttcaccat gtgcagtggc
     4981 aacaaaaact ggcgcgcagc ctgcaaatcg gtcgtgccag caacggcgga ctgccgaaac
     5041 gagatgtgaa tacggtcagc gaacctgaca gtcaaattcc accaggattc agagggtaac
     5101 gatgaaaaac gttggcgacc tgatgcaacg cctgcaaaaa atgatgcctg cccatatcaa
     5161 acctgcattc aaaacgggtg aagaactact ggcctggcag aaagaacaag gagcgatccg
     5221 ctccgccgct ctcgaacgtg aaaatcgggc gatgaaaatg cagcgcacct ttaaccgctc
     5281 cggtattcgg ccactgcatc agaactgctc ctttgagaac tatcgcgttg agtgtgaagg
     5341 gcagatgaat gcgttaagca aagcgcgcca gtacgtcgaa gagtttgacg gcaacatcgc
     5401 cagcttcatc ttttctggta agccaggaac cggcaaaaac catctggcgg cggcaatctg
     5461 caacgagctg ctgctacgcg gtaaatccgt attgatcatc accgtggccg atattatgtc
     5521 ggcgatgaaa gataccttca ggaatagcgg taccagtgaa gaacaactgc ttaacgatct
     5581 gagcaacgtt gatctgctgg tgatcgatga gatcggcgtg cagaccgaat cgaaatacga
     5641 aaaagtgatc atcaaccaga tcgtcgatcg ccgctcttct tccaaacgcc caaccgggat
     5701 gctgaccaac agtaatatgg aagagatgac caagttgctg ggcgaacgcg tgatggaccg
     5761 tatgcgcctg ggtaacagtt tgtgggtgat cttcaactgg gatagctacc gtagccgggt
     5821 aacaggtaaa gagtattaat ccccgtttct ggcggtatac tgcgacacaa ttccaccttg
     5881 agtaatgatg atggaaactg ttaaacatct tctgtgctgt gccattgccg ccagcgcatt
     5941 aatttccacc ggggtgcatg ctgcgtcctg gaaagatgcg ctctccagcg ccgccagcga
     6001 acttggcaac caaaacagca cgacacagga aggcggttgg tcgctcgcgt cattaactaa
     6061 cttgcttagc agcgggaacc aggccttaag cgcagataac atgaacaacg ccgcaggcat
     6121 tctgcaatac tgcgcgaagc aaaagctggc gtcggtaacc gatgccgaaa acatcaagaa
     6181 ccaggtgctg gaaaagctgg gcctgaacag tgaagagcaa aaagaagaca ccaactatct
     6241 ggatggcatt cagggtttgc tgaaaaccaa agatggtcag caactcaatc tggataacat
     6301 cggaacgact ccgctggcag aaaaggtgaa aaccaaagcc tgcgatctgg tgttaaagca
     6361 ggggctgaac ttcatttcct gaaccgcgaa acgccgcgtt ttagctgtta cccggctgac
     6421 gcggctctct cctgcccttt tccctgcttc tggacggttt tccccctgtt acacgggaag
     6481 cgaccgcgat caaaaaagtc tgtttataaa ctaattccta acaacatcgc atttttttga
     6541 cactagaatt gcagcaatat ggcgacgcca ttacattgtt cttctcaaaa agcttaaacg
     6601 ttggctaaac ggcccggttt agtgaggatc gcccgttgtc agaactactc tctttcgccc
     6661 tttttctcgc ctctgtgctg atttacgcat ggaaagcggg acgtaacacc tggtggtttg
     6721 cagccacatt aacggtgctg gggctatttg tcgttttaaa tatcaccctg tttgccagcg
     6781 actattttac tggcgatggt attaacgacg cggttctcta taccttaacc aacagcctga
     6841 ccggtgctgg cgtcagcaaa tacatcctgc cgggtatcgg cattgtgctg gggctgacag
     6901 cggtgttcgg tgcgctgggc tggatcctgc gccatcgtcg ccatcatccg caccattttg
     6961 gttacagcct gctggcgctc ttactggcgc tgggttcggt ggacgccagc ccggcatttc
     7021 gtcagataac ggaactggtg aaatcccagt cacgcgatgg cgacccggac tttgcggctt
     7081 attataaaga gccgtcgaaa actatccctg acccgaaact caacctggtt tatatctacg
     7141 gcgaaagtct cgagcggacc tattttgata acgaagcttt cccggatctc acgcctgaac
     7201 tgggcgcgtt gaaaaatgaa ggcctggatt tcagccacac gcagcagctg ccaggaacgg
     7261 attacacgat tgcgggcatg gtggcttctc agtgcggcat accgctgttt gccccctttg
     7321 aaggcaacgc ctccgcctct gtctccagct tcttcccgca gaacatctgt ctgggcgata
     7381 tcctgaaaaa ctcgggttat cagaactatt tcgtgcaggg cgcgaatctg cgttttgccg
     7441 gtaaagatgt gttcctgaag tcgcatggct tcgaccactt atacggctca gaagagctga
     7501 aaagcgtggt ggccgacccg cactatcgca acgactgggg attctacgac gataccgttc
     7561 tcgatgaagc gtggaaaaag tttgaagagc tttcccgctc aggtcagcga ttctcactgt
     7621 ttaccctgac agtcgatacc catcacccgg atggttttat ctcgcgtacc tgtaaccgca
     7681 aaaaatatga ttttgacggt aagccgaacc agtcattcag cgcggtaagt tgcagccagg
     7741 agaatatcgc ggcgtttatc aacaaaatca aagcgtcacc gtggtttaaa gataccgtca
     7801 tcgtcgtctc ttctgaccat ttagcgatga acaacacggc gtggaaatac ctcaataagc
     7861 aggatcgcaa taacctgttt tttgtcattc gtggcgacaa gccgcagcaa gagacgctgg
     7921 cagtgaagcg taacacgatg gataacggcg cgacggtgct ggacattctc ggtggcgata
     7981 actatctcgg acttggtcgt agcagtttat ccgggcagtc gatgtcggaa atcttcctca
     8041 atatcaaaga gaaaacattg gcgtggaagc cggatatcat ccgcctgtgg aaattcccta
     8101 aagagatgaa agagttcacc atcgaccagc agaaaaacat gattgccttc tcgggtagcc
     8161 atttccgttt gccgctgctg ttgcgggttt cagacaaacg cgtggaaccg ctgccggaaa
     8221 gcgaatactc agcaccgctg cgtttccagc tggccgattt cgctccacgc gacaatttcg
     8281 tctgggttga ccgttgctat aagatggcac aactctgggc tccggaactg gcactctcca
     8341 ccgactggtg tgtctcgcaa gggcagcttg gcggtcagca aattgttcaa catgttgaca
     8401 aaacaacatg gcagggcaaa acggcattta aagatacggt catcgacatg gcgcgttata
     8461 aaggcaatgt cgatacgctg aaaattgttg ataacgatat tcgctacaaa gccgacagtt
     8521 tcatcttcaa cgtcgccggt gcgccagaag aggtgaaaca gtttagtggg atttcccgtc
     8581 cagagtcgtg gggccgctgg tccaacgcgc agctgggcga tgaagtaaaa atcgagtaca
     8641 agcatccgct gccaaagaaa tttgacctgg tgattaccgc caaagcatac ggcaataacg
     8701 ccagccgtcc tattccggta cgcgtaggca atgaagaaca aacccttgtg ctgggcaatg
     8761 aagtgaccac caccacactg catttcgata acccaaccga tgccgacaca ctggtaattg
     8821 tgccgccgga acctgtctca accaacgaag ggaatatcct cggacactcg ccacgtaagc
     8881 tcgggatcgg catggtggaa attaaagtgg tggaacgtga aggataattc ctgactgatg
     8941 tgtaggtcgc ataaggtgcg acctacacac cttctgcggc ctacaaaaca tcattgagaa
     9001 acagaaaaaa acagcgtcca acacttcccc ggacgctgca tggcaacttt aataatctcg
     9061 ccttatcaga aagtaattac acccttaatc aactcacgat tgttaatcac atcgcgctcg
     9121 taggtttctg ccagcgtggc gaacggatag cgatgggtta acatcatgtc agcagtgatt
     9181 tttccttccg ccatcagtcg accgacttta gcaaaatctt ccggcgtggc gttgcggcta
     9241 cccatcatcg tcgtttcttt tttatggaat tccggatcgg agaactgcaa ctcaccttta
     9301 aacaggccga caaataccac cgtgccgccg tgacgaatca aattcacggt gttattcatc
     9361 gcatgttgat tacctgtcgc gtcgatcact ttctgcgcca gcgaaccacc aaactgcgcc
     9421 cgtagctgcg cgtcaaaatc ttcggctgac gggtccagta aaggtaattc cagacgcgtt
     9481 gccacatgtt cacggcgcgc cggactggta tccgccacca ccacctgtgc gccatcggct
     9541 ttagcgattg ccgccgcgcc cagaccgatt ggccccgccc cgaccaccag cacctgctcg
     9601 ccgggagcaa tggctgcgcg acgcaccgca tgagcgctaa tagcgaaagg ttcaatcaat
     9661 gctgccgcct gcgggtcaat accgtctgcg ggcaaaatgt tcgccaccgg cacgctcaaa
     9721 tactcactaa aaccgccatc ctgatgcacg ccaatgactg aaattttttc acagcaattg
     9781 gtacgcccgc ttttacacgc cgggcattgc tgacaggcaa cataagggat cacagcaact
     9841 tgcagaccat ttttaagatc agcaatattt ttacccagcc taacaatctc cccacatatt
     9901 tcatggccta aaacacgtgg ataactaaaa aatggttgat ttccacccca ggcatgaata
     9961 tcggtaccgc aaatcccgac agacttaatt tttattaatg cttcattgtc acccggaata
    10021 ggtatctcgc gttgtttcca gactaattct ttcggctgct ggcaaattaa aacattcatc
    10081 gtagacatga ttcggctctc cattttttgt tgccttagat atcaatgaaa atgaaaaatg
    10141 gcagcggaac ggaaaatctg ttttgtgaaa acacacgcat aaaaatgttt taaatcgggt
    10201 tttaatggac tcacaaggag gaaaatatga gtcgttcaca aaatttacgc cacaatgtga
    10261 ttaaccaggt cattgatgat atggcgcggg gtcatatccc ttccccactt ccatcgcaaa
    10321 gtgcgctggc ggaaatgtac aacatcagcc gcaccacagt gcgtcatatt ctcagccatt
    10381 tacgcgaatg cggcgtcttg acgcaggttg gaaacgacta tgttattgcg cgtaaacctg
    10441 accacgatga cggtttcgcc tgtactaccg cttcaatgag tgaacaaaac aaagtgtttg
    10501 agcaggcttt tttcacgctg ataaaccagc gccagttacg cccaggggaa actttttctg
    10561 aactgcaact ggctcgggcc gcgggtgtca gcccggtggt tgtaagagaa tatcttttaa
    10621 aattcggccg ttataatctt attcaaagcg aaaagcgcgg ccagtggagc atgaagcaat
    10681 tcgatcaatc ctacgccgag cagctgtttg agctacgcga aatgctcgaa acacattcac
    10741 ttcagcactt tctcaacctg cctgatcatg atccccgctg gctgcaggcg aaaaccatgc
    10801 tcgaacgtca tcggctactg cgtgacaaca tcggcaatag ctttcgcatg ttctcgcaac
    10861 ttgacagaga ttttcactcc ttactgcttt cagctgccga caatatcttt tttgatcaat
    10921 cgcttgagat catctcagtc atctttcatt ttcactatca atgggacgaa agcgatctta
    10981 aacagcgcaa tatcattgcc gtcgatgagc atatgaccat tctcagcgct ctgatttgcc
    11041 gcagtgacct cgatgccacc cttgcgctcc gtaaccattt gaattcagct aaacaatcaa
    11101 tgattcgatc cattaatgag aacacgcgtt atgctcatta agtacggatt aaaaacagca
    11161 aactattagc gggtgcacgg ttttatcaaa agataaatac cgggaaattc ccgcttacct
    11221 atgctcacaa tcagtcgact aaaaatgttt ttgtgagcgg tagtaaagtc ctaaaacttt
    11281 aacctgccgt taatgtcaac cttgcgtaat gcacaattac gtaaacaatt aaaattataa
    11341 atacaggagt ccggcgtgga aaaagaaaat atcaccatcg atccgcgttc ttcatttact
    11401 ccatcttcgt cggcagatat tcccgtgcca ccagatggat tagttcaacg cagtacccga
    11461 attaaacgca ttcaaaccac cgccatgttg ttattatttt ttgcggcggt aatcaattat
    11521 ctcgaccgca gttcgctgtc ggtagcaaat ttaacgattc gtgaagaatt gggattaagt
    11581 gccactgaaa tcggcgcttt gctctccgtg ttttcactcg cttacgggat tgcgcaactt
    11641 ccttgcggcc cactattgga tcgtaaaggc ccacgcctga tgctgggact ggggatgttc
    11701 ttctggtcac tgttccaggc aatgtctggc atggtgcaca actttacgca gttcgtgttg
    11761 gtgcgtatcg gtatggggat tggtgaagcg ccgatgaacc catgcggtgt aaaagtcatt
    11821 aacga
//
LOCUS       DADLFA010000128        11785 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000128 DADLFA010000000
VERSION     DADLFA010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11785
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.128"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            30..1415
                     /locus_tag="JQH65_002921"
     CDS             30..1415
                     /locus_tag="JQH65_002921"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAY6140278.1"
                     /translation="MEGGWNEGGKGISIADVMTAGAHGVPREVTEGVIDGLNYPNHEA
                     IDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEPNEEGLQFYDDLFDECLKQ
                     GMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTVFTRYKEKVKYWMTFNEIN
                     NQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYELVASALAVQTGKSINPEFN
                     IGCMIAMYPIYPLTCVPNDMMMATKAMHRRYWFTDVHARGYYPQHMLNYFARKGFNLD
                     ITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVEPRDLVSNPYIDTSEWGWQ
                     IDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTVNDHYRIDYFASHIREMKK
                     AVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKDNEGKGTLERIRKASFYWY
                     RDLIANNGENI"
     gene            complement(1472..1690)
                     /gene="marB"
                     /locus_tag="JQH65_002922"
     CDS             complement(1472..1690)
                     /gene="marB"
                     /locus_tag="JQH65_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HAY6140279.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(1722..2105)
                     /gene="marA"
                     /locus_tag="JQH65_002923"
     CDS             complement(1722..2105)
                     /gene="marA"
                     /locus_tag="JQH65_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HAY6140280.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            complement(2125..2559)
                     /gene="marR"
                     /locus_tag="JQH65_002924"
     CDS             complement(2125..2559)
                     /gene="marR"
                     /locus_tag="JQH65_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HAY6140281.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            2771..3436
                     /gene="marC"
                     /locus_tag="JQH65_002925"
     CDS             2771..3436
                     /gene="marC"
                     /locus_tag="JQH65_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HAY6140282.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            complement(3461..4651)
                     /gene="ydeA"
                     /locus_tag="JQH65_002926"
     CDS             complement(3461..4651)
                     /gene="ydeA"
                     /locus_tag="JQH65_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HAY6140283.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(4801..5508)
                     /gene="yneK"
                     /locus_tag="JQH65_002927"
     CDS             complement(4801..5508)
                     /gene="yneK"
                     /locus_tag="JQH65_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HAY6140284.1"
                     /translation="MGRVFHTGVERVLFLFLNDFIEQFPMIHLGVPIKKAHTPHIEPL
                     PPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWSNSEVHRWFVNKDPNLITAILDIT
                     DGELKEDLLQSLMDSLGSNKHVLPEVCICFLSLLAEQESPHFQDFFLFFANMLLHYHQ
                     FMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKNETPPKVTHEDLVKNRPRSPV
                     RPPIPATAKTPDLPERH"
     gene            complement(5457..5915)
                     /gene="yneK"
                     /locus_tag="JQH65_002928"
     CDS             complement(5457..5915)
                     /gene="yneK"
                     /locus_tag="JQH65_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001598467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HAY6140285.1"
                     /translation="MVTPVSISNYIFLPDDFPVRNIAPQVKEVLKNFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLNPIYLDNFRHDGEINIFALPTQSLSLWGAYFTPASSVCSFCF"
     gene            complement(5993..6874)
                     /locus_tag="JQH65_002929"
     CDS             complement(5993..6874)
                     /locus_tag="JQH65_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY6140286.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAIVLRVPSNLTIRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSAKQGYQ"
     gene            6975..8363
                     /gene="sad"
                     /locus_tag="JQH65_002930"
     CDS             6975..8363
                     /gene="sad"
                     /locus_tag="JQH65_002930"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HAY6140287.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALQLAAAGFRD
                     WRETNIDYRAEKLRGIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGGFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            8427..9353
                     /gene="glsB"
                     /locus_tag="JQH65_002931"
     CDS             8427..9353
                     /gene="glsB"
                     /locus_tag="JQH65_002931"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HAY6140288.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            9353..9712
                     /locus_tag="JQH65_002932"
     CDS             9353..9712
                     /locus_tag="JQH65_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HAY6140289.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            9851..11269
                     /locus_tag="JQH65_002933"
     CDS             9851..11269
                     /locus_tag="JQH65_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY6140290.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIVAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            11496..11735
                     /locus_tag="JQH65_002934"
                     /pseudo
     CDS             11496..11735
                     /locus_tag="JQH65_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="catalyzes the formation of D-tagaturonate from
                     D-altronate; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
BASE COUNT         2988 a         2663 c         3031 g         3103 t
ORIGIN      
        1 tatggggcgg cgcggtagcc gcgcatcagt tggaaggtgg ctggaatgaa ggaggaaaag
       61 gcatcagtat cgctgatgtg atgactgctg gcgctcacgg ggtgccgcgt gaagtgacag
      121 aaggcgttat cgacgggctt aattatccca atcatgaagc aattgatttt tatcatcgct
      181 ataaaacaga tattcagtta tttgccgaga tgggattcaa atgctttcga acttccattg
      241 cctggacacg aatctttccg caaggtgacg aacaggagcc gaatgaagag ggtttacaat
      301 tttatgatga tctgttcgat gaatgcctta agcagggaat ggaacctgtg gtgacgcttt
      361 cacattttga gatgccttat catctggtga caaaatatgg tggctggcga aaccgtaaac
      421 tgatcgactt ttttatccgc ttcgcatcaa cggtcttcac gcgctataaa gaaaaagtaa
      481 agtactggat gacgtttaac gaaatcaata atcaggtgaa tttcagcgaa agcctgtgtc
      541 catttactaa ttccggtatc ttgtattcgc cagaggaaga tatcaatgag cgcgaacaaa
      601 taatgtacca ggcggtacat tacgagttag ttgccagtgc cctggcggta cagactggaa
      661 aatcgatcaa tcctgaattt aacattggct gtatgatcgc catgtatccc atctatcctc
      721 tgacgtgtgt acccaacgat atgatgatgg ccacgaaagc aatgcatcgt cgttactggt
      781 ttactgatgt tcatgctcgt ggatattatc cgcaacatat gctgaattac tttgccagga
      841 aaggattcaa cctcgatatc acaccagaag ataacgcgat tcttgccagt ggttgtgtcg
      901 actttatcgg ctttagctac tacatgtctt ttacgacaca attttcgccc gataacccgc
      961 aactggatta tgttgaacca cgagatttgg tcagcaaccc ttatatcgat acatccgaat
     1021 ggggatggca aattgatccg gcagggctac gttattcact caactggttc tgggatcatt
     1081 tccagttgcc gctgtttatt gtcgaaaatg gatttggtgc ggttgaccag agacaagctg
     1141 acggcacggt gaacgatcac tatcgcattg attactttgc ttcccatatt cgggaaatga
     1201 aaaaagccgt agttgaagat ggtgttgact taattggcta caccccgtgg ggctgcattg
     1261 acctggtttc tgccggaaca ggggaaatga aaaagcgcta cggaatgatt tatgtcgata
     1321 aagacaacga agggaaggga acgctggaac ggatacgtaa agcgtcgttt tactggtatc
     1381 gggatctcat cgccaacaat ggcgaaaata tttgagactt gaggaagata ttacggagat
     1441 gtcaggctct gtgatcagag cctgacaata actacatagc gtgttgatta taatagggca
     1501 cgccgagcgc atccgactta tcactgccag tacccatgtg atttaaatca aacggtggtt
     1561 gttcctgcga tgggggaaca accacgacat tggcacaaga agtaacaact ggctgcgtgg
     1621 tttgttccgc aacgccctgc gcggaaaaga gaataagcgc agctgctatt gcggatgaaa
     1681 gtggtttcat gattgcctca gtgacgttgt cacgttatca actagctatt gtaatgattt
     1741 aatggatgta aaaagcgcga ttcgccctgc atattggtca tccggtattt atgtggcgga
     1801 acatcaaagt aatttttgaa ggttcgggtc agagtttgtt gcgactcgaa gccatatcgt
     1861 tctgccagat agagtatcgg ctcgttactt tccttcagct tttgcgcgat ttccgtcatc
     1921 ttacggctgc ggatgtattg gcctaatgaa tgaccggttt cttttttaaa catccgttgc
     1981 aggtgccatt tagagtaacc tgaacgctct gacactttct ccagtgacag tggcgattcc
     2041 aggttgtcct cgatccagtc caaaatgcta tgaatggtaa tagcgtcagt attgcgtctg
     2101 gacatcgtca tacctctttt ttgtttacgg caggactttc ttaagcaaat gctcaagtgt
     2161 tgccacttcg tccgccgtca ggttttttgt taattcttga tgcaggtcct ggccaactaa
     2221 ttgatggcat tgttcgcata ttgccgcgcc gctggtggta agttttacca gtacgccgcg
     2281 cttatcattc gggttcggca acctttcaac ccagccttta cagaccaggc gatccagcat
     2341 acgggtcagt gctccaaggt cgacagacaa cacttttttc agttcaaccg gagtaataca
     2401 cgccgcgcag cggatagagc agagcacctt aaactgtgcc gcggtaatat ccagcggaga
     2461 cagatactcg ttaagcagac gatctttctt ctgattaacc atatggatta agcgacccaa
     2521 tggaataatt tcattgaaca gatcgctggt acttttcaca ttagttgccc tggcaagtaa
     2581 ttagttgcag ggggtaatat tgcccaggca agtataagtc aaataaatga attgaccgat
     2641 gccacgtttt gctaaatcgg ttcaagtttt ggtcaatgcg atgcaaggtt agctaaagag
     2701 tgacacttta tccttgttta ccccttatac ttttcgctga taacccagat acactggata
     2761 acaaccacca atgttagatt tgtttaaagc aattggcctg gggctggtgg tgttactgcc
     2821 gttagctaac ccattaacaa ctgtcgcgtt atttcttggc ctggcaggca acatgaacag
     2881 tgccgaacgt aatcgtcagt cgttgatggc ctcggtgtac gtatttgcca tcatgatggt
     2941 ggcgtattac gccgggcaac tggtgatgga tacatttggc atttcgattc ccggtctgcg
     3001 aattgcaggc ggcttaatcg tcgcgtttat cggttttcgg atgctctttc cgcaacagaa
     3061 agcgattgat tcaccggagg cgaaaagcaa gtcggaagag ctggaagatg aacccagtgc
     3121 caatattgcc tttgtgccgc tagcaatgcc aagtaccgct ggtccgggaa ccatagcgat
     3181 gattattagc tcggcgtcca cagtgcgtca gagttcaacc ttcgctgatt gggtactgat
     3241 ggttgcgccg ccgctgatct ttttcctggt ggcggttatt ttgtggggaa gcctacgcag
     3301 ctccggcgca attatgcggc tggtgggcaa gggtggcatt gaagcgatct cccgtctgat
     3361 gggattcctg ctggtatgta tgggtgtgca gtttattatt aacggcatcc tggaaatcat
     3421 taaaacgtac cattaaaaaa ggcccgaatg ggcctttcaa ctattgcgtc tgttcttcga
     3481 gtgtcactgg ccagcggcga aatataatga ttgaccatat taacgcggca aaagcaggca
     3541 ccgcgcccac ataaccaatc atcgacattg accagtgcaa actcacctga ttacctacca
     3601 acgcacccgc cccgattcca atattaaata tgccggagaa tagcgccatc gcgacgtcgg
     3661 tagcatctgg tgccagcgcc agcactttaa cctgcatacc aagcccgatg atcatcatcg
     3721 cgatccccca gaaaatactc agcaccccga ggtgtatttc actgttcgcc gcaggtagca
     3781 gcagtgccag acacaccagc aacagcgcaa tcgccgtact caccaacgca gacgcatact
     3841 gattacccag tttaccgaaa atcacgctgc caataatgcc cgcaccaccg agtaataaca
     3901 gtaatgccgt ggcaaagttg gcgctgaatc ccgcaatgtt ttgcacaaaa ggctcgatat
     3961 agctgtatgc cgtgtaatgg gcggtgacaa ccaccacagt taacaaataa atgctcatca
     4021 atgccgggcg gcggaacaat agcgggaggc ttttcaatga accggaatgc tcactgggca
     4081 gtaagggaag taacttaatc aggcacaaaa gggtgataag cgccccaata ccaatcgcga
     4141 agaaggtcat tcgccaaccg aaatactgcc ccacaatgcg cccgagaggt aaacctaaga
     4201 ccatcgccag tgctgtaccg gtggcaatta aactcaaggc ctgtgcccgc ttcccggccg
     4261 gagccatacg gatcgccaga gacgccgtaa tcgaccagaa aatcgcatgt gcaaaagcca
     4321 caccaatgcg actgatcacc agaacggtaa agctccacga taaaaacgac agtacgtggc
     4381 tggcaataaa caccacaaac aggcagatca gtaatttgcg ccgttcaacc tgactggtca
     4441 ttaacataaa aggcaatgac attagcgcta ctacccatgc gtaaatggtc aacatgatgc
     4501 cgacctgagc ggtttgcatg tgaaaacttt gcgcaatgtc agagagcagg ccaacaggga
     4561 caaattcggt ggtgttgaag atgaaggcgg cgactgccag cgtaacgacc cgtagccacg
     4621 ccactttgcg ggaaacagtg tttgttgtca taacagatta tcgggtgagt tgcgaaaaag
     4681 tgatgaaatg atcttaaaac ggttaatgat gtgaatacaa tgcatttgtg atctggatcg
     4741 cgttttccgt ttctctgacg ctgattctgt tccgcgtcag agagtcggta acatcacggt
     4801 ttaatgacgt tcagggaggt ctggcgtttt ggcggttgcg ggtataggtg gtctgacagg
     4861 ggagcgaggc ctgtttttca ccaggtcttc gtgagtaact tttggcggcg tttcattttt
     4921 tacaaacagt ttgagggtcc tgcgcgccat atgcttgata attttatcat cacttaatga
     4981 tgctggcatc aacacgtcat tcagatcact ttcattggga ttcataaatt ggtgatagtg
     5041 caataacata ttggcgaaaa ataaaaaaaa gtcctgaaaa tgaggagatt cttgttctgc
     5101 taaaagggat aaaaagcaga tgcagacttc gggtagtaca tgtttgttag aacctaatga
     5161 atccatcagg ctttgcaata aatcctcttt taattcgccg tctgttatgt cgagaatggc
     5221 ggtgatcaag ttaggatctt tattgacgaa ccatctgtga acctcagagt tactccataa
     5281 tcgggggagt atgacaaaat taccgcgaga gataaaattc aggacaagca aatcaaactg
     5341 gcgtaagtaa tctgcagcgg tatggtgatc cggaggcagg ggttcaatat gtggtgtatg
     5401 cgcttttttg atggggacgc caaggtggat cattggaaat tgttcaataa aatcattcag
     5461 aaacagaaag agcacacgct cgacgccggt gtgaaatacg cgccccatag tgacaggctt
     5521 tgggttggga gcgcaaaaat attaatttct ccatcatggc gaaaattatc taaataaata
     5581 gggtttaaat ttattactac ctgaaattca atattttctt tgtgtcttcc attcttgacg
     5641 ggataagtga gtttaaactg aaccttttca ttgtatagtg tgtcgttacc ccgaaaagaa
     5701 gtcctctgaa tatatgataa gttatcaagg ttgttaaata tcttttttgt ggctgagttg
     5761 gtgtttaaag acgtgcgcca ctcttcatca catattatgg tactgagtgc atcaataaaa
     5821 ttctttaaaa cttcttttac ctgaggtgca atattacgta cgggaaaatc atcaggaaga
     5881 aatatgtaat tactgatgct tactggcgta accatatttg ccccatatag tcataagtca
     5941 tcggaacatc actatatagg gcatttgagc tgcaagaact atcaaaaata gctcattggt
     6001 aaccctgttt tgccgagtca ggcacatcca gtagttgaat aaatgcctca agcggacgcg
     6061 ttttcgcacc acgacgccag accagccagg ttgttaacca acgccattgc tcagctaacg
     6121 gccacgcttc aacctggtga tgccccggca tactttccag catagagcgt ggaataagcg
     6181 caatgcctgc tcctgcgacc acacaggcca acatcccgtg atacgactcc atctcgtgaa
     6241 tagttcccgg agcggcaccg tcagcatgaa accagctctc gaagtggcga cgatacgaac
     6301 aattggcgcg gaaggcataa atgttactgc cgttaacctg actggcgcgg attactggcg
     6361 catgaccttg tggcgtgacg atcatcagct cctcgcggta taccggtatc ccgtcaatgg
     6421 cagtatggtt aatgggccca tcaataaatg ccgcattcag ttttccctcc agcactcctt
     6481 ccagcatagt gccggaaggg ccggtggaaa gtgaaaactg aattttggga taacgatggt
     6541 tgaattccgc cagcgtggcc ggaatgcgca ctgctgcggt gctttccagc gaaccaagag
     6601 aaaataaacc ttgcggctca tcgccagcga caacgctccg cgcttcatcc actaacgcga
     6661 gaatttgttg gctatagcgt aaaaagttat gcccggcagg agagagacgt aaacgctgat
     6721 tctcgcgaat aaacagatct acccccagtt ctgtttccag ctggcgtaga cggatagtga
     6781 gattcgacgg cacgcgaagc actattgctg ctgcctgggt tatgctgcca gcttcggcaa
     6841 ccgcgttgaa catctccagt tgggtcagat ccataatatt ctcgattcgt gaataagtgg
     6901 cttaatatta ttcattttaa agcaagagta aatctgcgta tcttcatacc atgactcata
     6961 aaggagatac cccgatgacc attactccgg caactcatgc aatttcgata aatcctgcca
     7021 cgggtgaaca actttctgtg ctgccgtggg ctggcgctaa cgatatcgaa aacgcacttc
     7081 agctggcggc agcaggcttt cgcgactggc gcgagacaaa tatagattat cgtgctgaaa
     7141 aactgcgtgg tatcggtaag gctctgcgcg cccgtagcga agaaatggcg caaatgatca
     7201 cccgtgaaat gggcaaacca atcaatcagg cgcgcgctga agtggcgaaa tcggcgaatt
     7261 tgtgtgactg gtatgcagaa catggtccgg caatgctgaa ggcggaacct acgctggtgg
     7321 aaaatcagca ggcagttatt gagtatcgac cgttggggac gattctggcg attatgccgt
     7381 ggaactttcc gttatggcag gtgatgcgtg gcgcggttcc catcattctt gcaggtaacg
     7441 gctacttact taaacatgcg ccgaatgtga tgggctgtgc tcagctcatt gcccaggtgt
     7501 ttaaagatgc gggaatcccg caaggcgtat atggctggct gaatgccgac aacgacggtg
     7561 tcagtcaaat gattaaagat tcgcgcattg ctgctgtcac ggtgaccgga agtgttcgtg
     7621 cgggagcggc tattggcgca caggctggag cggcactgaa aaaatgcgta ctggaactgg
     7681 gcggttcgga tccatttatt gtgcttaacg atgccgatct ggaactggcg gttaaagcgg
     7741 cggtagccgg acgttatcag aataccggac aggtttgtgc agcggcaaaa cgctttatta
     7801 tcgaagaggg aattgcttct gcatttaccg aacgttttgt ggcagctgcg gcagccttga
     7861 aaatgggcga tccccgtgat gaagagaacg ctctcggacc aatggctcgt tttgatttac
     7921 gtgatgagct gcatcatcag gtggagaaaa ccctggcgca gggtgcgcgt ttgttactgg
     7981 gcggggaaaa gatggctggg gcaggtaact actatccgcc aacggttctg gcgaatgtta
     8041 ccccagaaat gaccgcgttt cgggaagaaa tgtttggccc tgttgcggca atcaccgttg
     8101 cgaaagatgc agaacatgcg ctggaactgg ctaatgatag tgagttcggc ctttcagcga
     8161 ccatttttac caccgacgaa acacaggcca gacagatggc ggcacgtctg gaatgcggtg
     8221 gggggtttat caatggttat tgtgccagcg acgcgcgagt ggcctttggt ggcgtgaaaa
     8281 agagtggctt tggtcgtgag ctttcccatt tcggcttaca cgaattctgt aatatccaga
     8341 cggtgtggaa agaccggatc tgacgcgaca tattcagctc tgatatactc gcaggtcttt
     8401 tcagacctgc ggtccaggag tagaaagtgg cagtcgccat ggataatgca attttagaaa
     8461 acatcttgcg gcaagtgcgg ccgctcattg gtcagggtaa agtcgcggat tatattccgg
     8521 cgctggctac agtagacggt tcccgattgg gcattgctat ctgtaccgtt gacggacagc
     8581 tttttcaggc cggagacgcg caagaacgtt tttccattca gtctatttcc aaagtgctga
     8641 gtctcgttgt cgccatgcgt cattactccg aagaggaaat ctggcaacgc gtcggcaaag
     8701 atccgtctgg atcaccgttc aattctttag tgcaactgga aatggagcag ggtattccgc
     8761 gtaatccgtt tattaatgct ggtgcgctgg tggtctgcga tatgttgcaa gggcgattaa
     8821 gcgcaccacg gcaacgtatg ctggaagtcg tgcgcggctt aagcggtgtg tctgatattt
     8881 catacgatac ggtggtagcg cgttccgaat ttgaacattc cgcgcgaaat gcggctatcg
     8941 cctggctgat gaagtcgttt ggcaatttcc atcatgacgt gacaaccgtt ctgcaaaact
     9001 actttcatta ctgcgctctg aaaatgagct gtgtagagct ggcccggacg tttgtctttc
     9061 tggctaatca ggggaaagct attcatattg atgaacctgt ggtgacgcca atgcaggcgc
     9121 ggcaaattaa cgcgctgatg gcgaccagtg gtatgtacca gaacgcgggg gagtttgcct
     9181 ggcgggtggg gctaccggcg aaatctggcg ttggtggcgg tattgtggcg attgttccgc
     9241 atgaaatggc catcgctgtc tggagtccgg aactggatga tgcaggtaac tcgcttgcgg
     9301 gtatcgccgt ccttgaacaa ttgacgaaac agttagggcg ttcggtttat taatgcagtc
     9361 tctcgatcca ctcttcgcgc gtttatcccg ttcaaaattt cgctctcgct ttcgtctggg
     9421 catgaaagag cgtcagtatt gcctggagaa aggcgcgcca gtcatcgaac aacatgcggc
     9481 ggattttgtc gctaaacgtc ttgctccggc attaccggct aatgacggta agcaaacacc
     9541 catgcgcggg catccggtgt ttatcgctca gcacgccacc gcgacctgct gtcgcggttg
     9601 tctggctaag tggcataaca ttccgcaggg tgtttcgtta agcgaagaac aacaacgtta
     9661 tatcgtggcg gtaatctatc actggctggt tgtacagatg aatcagccat gagaattgcg
     9721 gaaaacgata aaatagcatt cccagggtac ctttatttgc cgccgctgtt agcgaacatg
     9781 ctatccttta tgagaattta cttattacgt tgagataatt gccacccgca attgtatgga
     9841 cagataatta atgcatgtac accccatttc aactttccgt ttgttccagg aaggtcatct
     9901 gctacgtaat agcatcgcta tttttgcgct aaccacgctg ttctatttta ttgttgcaga
     9961 gttacggctg gttcacgaac tttctctttt ctggccgctg aatggcgtaa tggcgggggt
    10021 gtttgcccgc tatgtctggc ttaatcgact gcattactat gcgatcagtt atgtggcgat
    10081 gctggtttat gatgccataa ccaccgaatg gggactggtt tcactggtta tcaatttctc
    10141 caatatgatg tttattgtta ccgtcgcctt actggtcgtg cgggataagc gtctggggaa
    10201 aaataagtat gagccagtaa gtgccttacg gctatttaat tactgtctga ttgccgcatt
    10261 attatgcgct attgtcgggg cgattggttc ggtcagtatt gatagtctgg atttctggcc
    10321 tttgcttgcg gactggttca gtgagcaatt ctcaacgggc gtgttgatcg tgccttgtat
    10381 gctgacgttg gctattcctg gagtgctgcc gcgctttaaa gcagaacaga tgatgcccgc
    10441 catcgcgctt attgtgtcgg ttattgcctc ggtagtcatt ggcggagcgg ggagtctggc
    10501 gttcccgctg cctgcattaa tctggtgtgc agtgcgctat acgccgcagg taacatgtct
    10561 gttgaccttt gtcaccggtg cggtggaaat cgtactggtg gcaaattcgg tgattgatat
    10621 ctcggtcggt tcgccgttct ccattccgga aatgttctcc gcacgtctcg gtattgccac
    10681 gatggcgata tgcccaatta tggtttcttt tagcgtggca gcgatcaatt cgctaatgaa
    10741 gcaagttgcg ctgcgagccg actttgattt tctgactcag gtttactcac ggtccggtct
    10801 ttatgaggcg ctgaaaagtc catcgctgaa acagacacaa catctgacgg tcatgctgct
    10861 tgatatcgac tatttcaaaa gcattaacga taactatgga catgaatgtg gcgataaagt
    10921 gttaagcgtg tttgcccggc atattcagaa gattgtcggt gataaggggc tggtggcgcg
    10981 catgggcggc gaagaatttg ctgttgcagt gccgtcggtg aatcctgtcg atggtctgct
    11041 aatggcggaa aaaatccgta aaggcgttga actgcaacca ttcacctggc aacaaaaaac
    11101 gctttatctc acggtaagta ttggcgtcgg tagtggtcgc gcatcgtacc gaacgctgac
    11161 cgatgatttt aataaattga tggtcgaagc cgatacgtgt ctgtatcgct cgaagaaaga
    11221 tgggcgcaac cgtaccagca ctatgcgtta cggtgaggaa gttgtctgac atctttattc
    11281 attccgcgcg atttgaatgc aatgtgttct accactttat gatccaggaa aaggcgtatt
    11341 tcctcgatta aaccatgatc cgcgccacac ttttctcata aaactgctca ttaatcatca
    11401 ggagttgatc aaaacagggg ttcggcgtat taaattttgc taacctgtcg taccaattca
    11461 agcgggttgt atgactaatc agaagggaac ccattgtgaa aacactaaat cgtcgcgata
    11521 ttcccggtgc acagtatcca gaacgtatca ttcagtttgg tgaaggtaac ttcctgcgcg
    11581 cctttgttga ctggcaaatc gatctcctga atgagcacac cgatctgaat tctggcgtgg
    11641 tcgttgttcg tccgattgaa acttcattcc cgccgtcact gagcacgcag gatggtctgt
    11701 acaccaccat tatccgtggc ctgaatgaga aggggtgaag tggtcaacaa aaactggcca
    11761 ccgagttaga gtttttccag tatcg
//
LOCUS       DADLFA010000129        11775 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000129 DADLFA010000000
VERSION     DADLFA010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11775
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.129"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(11..661)
                     /gene="hxpA"
                     /locus_tag="JQH65_002935"
     CDS             complement(11..661)
                     /gene="hxpA"
                     /locus_tag="JQH65_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HpxA"
                     /protein_id="HAY6140291.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTCLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     AEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQLNGDVIIQ"
     gene            complement(672..1166)
                     /locus_tag="JQH65_002936"
     CDS             complement(672..1166)
                     /locus_tag="JQH65_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HAY6140292.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACLRQYHLSANEINQ
                     IINA"
     gene            complement(1250..1705)
                     /locus_tag="JQH65_002937"
     CDS             complement(1250..1705)
                     /locus_tag="JQH65_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HAY6140293.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            2043..3245
                     /gene="ackA"
                     /locus_tag="JQH65_002938"
     CDS             2043..3245
                     /gene="ackA"
                     /locus_tag="JQH65_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAY6140294.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            3320..5464
                     /gene="pta"
                     /locus_tag="JQH65_002939"
     CDS             3320..5464
                     /gene="pta"
                     /locus_tag="JQH65_002939"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAY6140295.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNQEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            5654..7174
                     /gene="yfcC"
                     /locus_tag="JQH65_002940"
     CDS             5654..7174
                     /gene="yfcC"
                     /locus_tag="JQH65_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAY6140296.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQITVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(7207..7749)
                     /gene="yfcD"
                     /locus_tag="JQH65_002941"
     CDS             complement(7207..7749)
                     /gene="yfcD"
                     /locus_tag="JQH65_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HAY6140297.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(7807..8358)
                     /gene="yfcE"
                     /locus_tag="JQH65_002942"
     CDS             complement(7807..8358)
                     /gene="yfcE"
                     /locus_tag="JQH65_002942"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HAY6140298.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(8414..9046)
                     /gene="yfcF"
                     /locus_tag="JQH65_002943"
     CDS             complement(8414..9046)
                     /gene="yfcF"
                     /locus_tag="JQH65_002943"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HAY6140299.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASVAMAEHLLALGQPNLFGEW
                     CIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            9182..9829
                     /gene="yfcG"
                     /locus_tag="JQH65_002944"
     CDS             9182..9829
                     /gene="yfcG"
                     /locus_tag="JQH65_002944"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HAY6140300.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGELGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            9886..10248
                     /gene="folX"
                     /locus_tag="JQH65_002945"
     CDS             9886..10248
                     /gene="folX"
                     /locus_tag="JQH65_002945"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HAY6140301.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            10269..11162
                     /locus_tag="JQH65_002946"
     CDS             10269..11162
                     /locus_tag="JQH65_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family protein"
                     /protein_id="HAY6140302.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIDSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(11210..>11775)
                     /locus_tag="JQH65_002947"
     CDS             complement(11210..>11775)
                     /locus_tag="JQH65_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HAY6140303.1"
                     /translation="GYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSA
                     FPLVDITVVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKA
                     LFNYVLQTGDAQRFRAFIGEIAERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQE
                     MLDRGLDRELVMMVTRLSPDDLIAQSH"
BASE COUNT         2818 a         3121 c         2945 g         2891 t
ORIGIN      
        1 atcaggtgtt tcactgaata ataacatcgc catttagctg tttggtcaca gtaatttgct
       61 ccagactgtg gaggaccaaa tcgacctcat tcaggcgcgg ggtatctgcc ggagcgttaa
      121 ccgcaatgac atggcaaccc gccgccaggc cagaaagcac gccagcggga gcatcttccg
      181 ccaccacaca ctcctgcggc gcaagcccca gcagctgcgc acctaacaga tacgcatcag
      241 gttctggttt tccgcgcttc actcgctcag cggttacaaa cacctctggt gcgggaagcc
      301 cagctatttt atggcgcgct cgcgctaccg gcatggagcc agaagtcaca atggcccacg
      361 gaatacctgc tttattcaaa tgattgagta aggcgattgc ccccggaagc gcggtaatac
      421 cttcggtttc cgtggcctcg atgtgctcca gacacgtaaa ctcggcggca atatcagcct
      481 cggatttgcc cgccataaaa tggcgcaaag aggtgatcgc ctgtttaccg tgaatgaaag
      541 ccagcacctc ttccggcgct aacccatgac gtctggccca gttgctccac gcccgttcta
      601 ccgcaggcag ggaatccacc agcgttccat caagatcaaa cagaaaacct ttgcaccgca
      661 cgcgggcctc ctcaggcgtt gataatttga ttaatttcgt tggcgctcaa atggtactga
      721 cgcaggcagg catgccacac attaagcatg cgctgatatt tttcccacat tggcgtctgg
      781 gcgttaaaac catgagtccc agcgtcaaaa tgggtatagc gcccttccac attaaccata
      841 aagcggacat aaccgaggta acgtgcttca gtggcggcgt caaagccgag gaaggtgaca
      901 cgacgttcat cgatggattg ctggtcctgc aaattagacc aggaaacatg caacgcatga
      961 tacatctcca taatgtcgat gatagtgcgg caggtttctt ctttcagctc gccaaactcg
     1021 cgatccaatt cacgcatctg taatccgtaa ccacgctcaa taattgtttg caggcgacgg
     1081 taacgttcag catttgccgg atcgagcata gtcatcatct tgtactgatt agacaaaata
     1141 agacgttgcg cgttggtcat ttccattgtt gactcctgta tcactctact acggtgaaaa
     1201 aaaaagaagg ctgagtatgc cttcttttat atgcgtaatc aggggtcaat tacaaatcat
     1261 caaggaaagt tttatccagt tgtttgaagg cgcgcttaag cgtgtcagct aatgcctggt
     1321 aatcaggctt gccttcaacg ggtgccaaca cctgtccaga ctcctgcaat ttaccgcgaa
     1381 cttcataaaa ccagttgagg atagcagggg gtaatggcgt tacagaacgc ttgcccagcc
     1441 accataatcc ctgcatgggt aaacttaagg cgaacagcgc agtggcaact gccggcccaa
     1501 gctgaccgcc cagggcaatc tgccagcaga gagtaaatac ggcgatcggc ggcataaaac
     1561 ggatcgcata acgcgtcatc ttgataacgc gattttcgac aaagaccggg gcaaggcgtt
     1621 tttccagcgg ccacgtcttt gagtaatgct gtccccggcg aaacaagcta aaaaaattaa
     1681 cagaacgatt atccggcgtt gacatgcttc acctcaactt cacatataaa gattcaaaaa
     1741 tttgtgcaaa ttcacaactc agcgggacaa cattcaaaac attttgtctt ccatacccac
     1801 tatcaggtat cctttagcag cctgaaggcc taagtagtac atattcattg agtcgtcaaa
     1861 ttcatataca ttatgccatt ggctgaaaat tacgcaaaat ggcatagact caagatattt
     1921 cttccatcat gcaaaaaaaa tttgcagtgc atgatgttaa tcataaatgt cggtgtcatc
     1981 atgcgctacg ctctatggct ccctgacgtt tttttagcca cgtatcaatt ataggtactt
     2041 ccatgtcgag taagttagta ctggttctga actgcggtag ttcttcactg aaatttgcca
     2101 tcatcgatgc agtaaatggt gaagagtacc tttctggttt agccgaatgt ttccacctgc
     2161 ccgaagcacg tatcaaatgg aaaatggacg gcaataaaca ggaagcggct ttaggtgcag
     2221 gcgccgctca cagcgaagcg ctcaacttta tcgttaatac tattctggca caaaaaccag
     2281 aactgtctgc gcagctgact gctatcggtc accgtatcgt acacggcggc gaaaagtata
     2341 ccagctccgt agtgatcgat gagtctgtta ttcagggtat caaagatgca gcttcttttg
     2401 caccgctaca caacccggct cacctgatcg gtatcgaaga agctctgaaa tctttcccac
     2461 agctgaaaga caaaaacgtt gctgtatttg acaccgcgtt ccaccagact atgccggaag
     2521 agtcttacct ctacgccctg ccttacaacc tgtacaaaga gcacggcatc cgtcgttacg
     2581 gcgcgcacgg caccagccac ttctatgtaa cccaggaagc ggcaaaaatg ctgaacaaac
     2641 cggtagaaga actgaacatc atcacctgcc acctgggcaa cggtggttcc gtttctgcta
     2701 tccgcaacgg taaatgcgtt gacacctcta tgggcctgac cccgctggaa ggtctggtca
     2761 tgggtacccg ttctggtgat atcgatccgg cgatcatctt ccacctgcac gacaccctgg
     2821 gcatgagcgt tgacgcaatc aacaaactgc tgaccaaaga gtctggcctg ctgggtctga
     2881 ccgaagtgac cagcgactgc cgctatgttg aagacaacta cgcgacgaaa gaagacgcga
     2941 agcgcgcaat ggacgtttac tgccaccgcc tggcgaaata catcggtgcc tacactgcgc
     3001 tgatggatgg tcgtctggac gctgttgtat tcactggtgg tatcggtgaa aatgccgcga
     3061 tggttcgtga actgtctctg ggcaaactgg gcgtgctggg ctttgaagtt gatcatgaac
     3121 gcaacctggc tgcacgtttc ggcaaatctg gtttcatcaa caaagaaggt acccgtcctg
     3181 cggtggttat cccaaccaac gaagaactgg ttatcgcgca agacgcgagc cgcctgactg
     3241 cctgatttca caccgccagc tcagctggcg gtgctgtttt gtaacccgcc aaatcggcgg
     3301 taacgaaaga ggataaaccg tgtcccgtat tattatgctg atccctaccg gaaccagcgt
     3361 cggtctgacc agcgtcagcc ttggcgtgat ccgtgcaatg gaacgcaaag gcgttcgtct
     3421 gagcgttttc aaacctatcg ctcagccgcg taccggtggc gatgcgcccg atcagactac
     3481 gactatcgtg cgtgcgaact cttccaccac gacggccgct gaaccgctga aaatgagcta
     3541 cgttgaaggt ctgctttcca gcaatcagaa agatgtgctg atggaagaga tcgtcgcaaa
     3601 ctaccacgct aacaccaaag acgctgaagt cgttctggtt gaaggtctgg tcccgacacg
     3661 taagcaccag tttgcccagt ctctgaacta cgaaatcgct aaaacgctga atgcggaaat
     3721 cgtcttcgtt atgtctcagg gcactgacac cccggaacag ctgaaagagc gtatcgaact
     3781 gacccgcaac agcttcggcg gtgccaaaaa caccaacatc accggcgtta tcgttaacaa
     3841 actgaacgca ccggttgatg aacagggtcg tactcgcccg gatctgtccg agattttcga
     3901 cgactcttcc aaagctaaag taaacaatgt tgatccggcg aagctgcaag aatccagccc
     3961 gctgccggtt ctcggcgctg tgccgtggag ctttgacctg atcgcgactc gtgcgatcga
     4021 tatggctcgc cacctgaatg cgaccatcat caacgaaggc gacatcaata ctcgccgcgt
     4081 taaatccgtc actttctgcg cacgcagcat tccgcacatg ctggagcact tccgtgccgg
     4141 ttctctgctg gtgacttccg cagaccgtcc tgacgtgctg gtcgccgctt gcctggcagc
     4201 catgaacggc gtagaaatcg gtgccctgct gctgactggc ggctacgaaa tggacgcgcg
     4261 catttctaaa ctgtgcgaac gtgctttcgc taccggcctg ccggtattta tggtgaacac
     4321 caacacctgg cagacctctc tgagcctgca gagcttcaac caggaagttc cggttgacga
     4381 tcacgaacgt atcgagaaag ttcaggaata cgttgctaac tacatcaacg ctgactggat
     4441 cgaatctctg actgccactt ctgagcgcag ccgtcgtctg tctccgcctg cgttccgtta
     4501 tcagctgact gaacttgcgc gcaaagcggg caaacgtatc gtactgccgg aaggtgacga
     4561 accgcgtacc gttaaagcag ccgctatctg tgctgaacgt ggtatcgcaa cttgcgtact
     4621 gctgggtaat ccggcagaga tcaaccgtgt tgcagcgtct cagggtgtag aactgggtgc
     4681 agggattgaa atcgttgatc cagaagtggt tcgcgaaagc tatgttggtc gtctggtcga
     4741 actgcgtaag aacaaaggca tgaccgaaac cgttgcccgc gaacagctgg aagacaacgt
     4801 ggtgctcggt acgctgatgc tggaacagga tgaagttgat ggtctggttt ccggtgctgt
     4861 tcacactacc gcaaacacca tccgtccgcc gctgcagctg atcaaaactg caccgggcag
     4921 ctccctggta tcttccgtgt tcttcatgct gctgccggaa caggtttacg tttacggtga
     4981 ctgtgcgatc aacccggatc cgaccgctga acagctggca gaaatcgcga ttcagtccgc
     5041 tgattccgct gcggccttcg gtatcgaacc gcgcgttgct atgctctcct actccaccgg
     5101 tacttctggt gcaggtagcg acgtagaaaa agttcgcgaa gcaactcgtc tggcgcagga
     5161 aaaacgtcct gacctgatga tcgacggtcc gctgcagtac gacgctgcgg taatggctga
     5221 cgttgcgaaa tccaaagcgc cgaactctcc ggttgcaggt cgcgctaccg tgtttatctt
     5281 cccggatctg aacaccggta acaccaccta caaagcggta cagcgttctg ccgacctgat
     5341 ctccatcggg ccgatgctgc agggtatgcg caagccggtt aacgacctgt cccgtggcgc
     5401 actggttgac gatatcgtct acaccatcgc gctgactgcg attcagtctg cacagcagca
     5461 gtaatctcgt catcatccgc agctttgcgc tgcggatatc tgaaccggaa ataatcacta
     5521 tttccggttt tttattctct taatctgcat taatcctttc tgattatctt gcttaactgc
     5581 gctgcatcaa tgaattgcgc catttcactt tgcatactta ccactttgtt ttgtgcaagg
     5641 gaatatttgc gctatgtccg caatcactga atccaaacca acaagaagat gggcaatgcc
     5701 cgatacgttg gtgattatct tttttgttgc tattttaacc agccttgcca cctgggtagt
     5761 tccggtgggg atgtttgaca gtcaggaagt gcagtatcag gttgatggtc aaacaaaaac
     5821 acgcaaagtc gtagatccac actcatttcg cattctgact aacgaagcag gcgaacctga
     5881 gtatcaccgc gtacagctgt tcacgacggg cgatgaacgc ccgggcctga tgaacttccc
     5941 gtttgaagga ttaacctcag gatcgaaata cgggacagct gttggcatca tcatgtttat
     6001 gctggtgatt ggcggcgcgt ttggcattgt gatgcgtaca ggaaccattg ataacggtat
     6061 cctggcgctt attcgccata cccgcgggaa tgaaattctc tttattcctg cgctgtttat
     6121 tctgttttca cttggcggcg cggtatttgg tatgggagaa gaggccgtcg cctttgccat
     6181 tatcatcgca ccgctaatgg tccggctggg ctatgacagt attaccaccg tcctggtgac
     6241 ctatattgcc acgcaaatcg gttttgccag ttcgtggatg aacccgtttt gtgtggtcgt
     6301 tgctcagggg attgccggcg ttccggtgct ttctggctcc gggttgcgca tcgtggtgtg
     6361 ggttatcgcc actatgattg gcctgatctt taccatggtg tacgcctcac gagtgaaaaa
     6421 gaatcctctt ctgtcacgcg tgcatgagtc cgaccgcttc tttcgtgaaa agcaggcgga
     6481 tgttgaacaa cgtccgttta cctttggtga ctggctggta ttgattgtcc tgaccgccgt
     6541 aatggtctgg gtgatttggg gcgtgatcgt taatgcctgg tttattccag aaattgccag
     6601 ccagttcttc accatgggtc tggtgattgg catcatcggc gttgttttcc gccttaacgg
     6661 catgacggtt aataccatgg cttcatcctt taccgaaggg gcgcgaatga tgatcgcccc
     6721 tgccctgctg gtgggtttcg ccaaagggat tttgctgctg gtcggtaatg gtgaagcggg
     6781 tgatgccagc gtgttaaata ccatcctcaa cagcattgcc aatgccatta gcggtctgga
     6841 caacgcggtc gcggcctggt ttatgttgct cttccaggcg gtatttaatt tcttcgtgac
     6901 gtccggttct ggtcaggcgg cgttaaccat gccgttactg gcaccgcttg gcgatctggt
     6961 cggtgttaac cgtcagatta ccgtgctggc attccagttt ggtgatggct tcagtcacat
     7021 catttaccca acctcagctt cgttaatggc aactctcggt gtgtgcaggg tggacttccg
     7081 taactggctg aaggtgggcg cgaccctact tggactgctg tttattatgt ccagcgtcgt
     7141 agtgatcggc gctcagttga tgggctacca ctaaaaatgt taagagccgc aattgcggct
     7201 cttttttcat tctgccgttt cagtctctac ggcttcattt ttggcattgc gcttcatcca
     7261 caacgccagc gctttcagcg agtctggagt gaactcatcg cagcgtgcgg tgatttcttc
     7321 cggcgtcagc cagcaaactt cactgacttc atcttcctgt agggcgaagg gaccgtgaga
     7381 gacgcagctg aacaacgcgc cccagacacg gcaattttta tcttcgaaat agaactgccc
     7441 gtgctcggca aaggggacac cggcaatgcc caactcttct tccgcttcgc gacgcgcgga
     7501 ttccagcagt tgctcatcgg cctgaaccac accgcctgcg gtcgcatcta acatgccggg
     7561 taaaaagtct tttgtctcgg tacgacgctg gaccagaatt ttgcccatgc catcatgcac
     7621 gacgatgtaa gttgcacgat gacgcagaca ctgtgcccgc atttgttccc ggctggcttg
     7681 tgcaatgact tcgttctctt cattgacaat atccacccat tcagtacttg ccaaacgacg
     7741 ctgttccatt atcaggaaac cttctttttc tggcgctctt acggcgcatt ttgagttgtg
     7801 ggtaaattac ggattaatcg cgacctgcgc aatgatactt tgatcattga gtgcgattac
     7861 actaagtacg tcattatcca gcatgccata actcgccgga ttaccgcctt tcggaatact
     7921 caccgagccg gggttgaagt ggaaaatctc cccccgctgt tctgccactg gcagatgggt
     7981 atgaccgtac accagcacat cgttctggtt taaagcaggt agattttccg ggccaaaaag
     8041 atgaccatgc gtcaaaaaca gacgttgttt ttccagtaat acctgttgcc acggcgcggt
     8101 tatcgggaaa tgcagcagca tttgatccac ttcgctgtcg cagttgccgc gcacagcgat
     8161 aaccttatgt gccacttcat taagccgttc agcgactttg gctggcgcgt aaccctccgg
     8221 taaagcatta cgcgggccgt gattcaacac gtcgccaagg atcaccagcc actgggcacc
     8281 gctttgggca aacaactcca gaacacgttc cgtcgccggt aacgacccat gaatgtccga
     8341 tgcaaacatc agtttcatca ctcactcctc gtcgaaaaaa cgtaagctat gatactggat
     8401 tctgtcgttg ctatcagcca gattgcttcg ccgaaagtgc aataaaacgc tggacagacg
     8461 ctcgctgcca ctggaatgtc gcataatcca ccaggcgttc tggcacctca tcgccatgta
     8521 gtaccaggcg gttaatcatt agcgccagat cagtatcagc aatgcaccat tcaccaaata
     8581 aattcggctg acccagtgct aacaaatgtt ctgccatcgc gacactggct tttccctcgg
     8641 ccgttagtgg cgcttttttc gcccccgcaa agacaacatc cgtcggacgc tcttcgcgga
     8701 tgggcatcag atcgctgcgc agccaggcct gaatctgtcg tgcacgcgca cgattttcta
     8761 aatcaagcgg ataaatacgt tcccaggtcg gtggcgcaaa tcgatcttcc agatactccg
     8821 caatggcaga agattcactc aactcaaaat catcgatttg taataacggc acacggcgtg
     8881 tctgaccgta accttgccac gtcggctgca aatgttcacc gctgtcgagg tcgatggtct
     8941 tgatatgaaa cgacaggcct ttttcctgca acgccaccca agcggataac acataagggg
     9001 agaaaaagtg ggcatctgac caaagcgtga tagcgggttt actcattatg tcctcggcaa
     9061 taaaggaggc tggctatcaa actatagagc ctttttgtca ctgtcacgtg acgaatactc
     9121 acgtaagtcg ccgcgctttc tatacttgtg gtgttcagat gattaaacaa ccggagctgc
     9181 aatgatcgat ctctatttcg ccccgacacc caatggtcac aaaattacgc tgtttctcga
     9241 agaagcagag cttgattatc gcttgattaa ggtagacctg gggaaaggcg gtcagtttcg
     9301 tccggaattt ttgcgcattt cgcccaacaa caaaattccg gcaattgttg atcattctcc
     9361 tgccgatggc ggcgaaccgc taagcctctt tgaatctggt gccattttgt tgtatctggc
     9421 tgagaaaacg gggctctttt tgagtcatga aacgcgtgaa cgcgccgcca cattacagtg
     9481 gttattctgg caggtaggcg aactggggcc gatgcttggg caaaatcatc attttaatca
     9541 cgcagccccc caaaccattc cttacgctat tgaacgttat caggttgaaa ctcagcgtct
     9601 ttaccatgta ctaaacaagc ggctggaaaa ctcgccctgg ctgggaggcg agaactacag
     9661 cattgcggat attgcctgct ggccgtgggt taatgcctgg actcgccagc gaattgacct
     9721 cgcaatgtat ccggcagtca agaactggca tgagcggatc cgttcgcgcc ctgccaccgg
     9781 gcaggcactg ctaaaagcac aactcggtga tgagcgttcg gatagttaac agaaacaggt
     9841 tctcgtgtat tatttcatcc taagtaaaac aacggagaac ctgcaatggc acaacctgcc
     9901 gctattattc gtataaagaa ccttcgttta cgtacgttta tcggaattaa ggaagaagaa
     9961 attaacaacc gtcaggatat tgttatcaat gtgacgatcc actaccccgc cgataaagcg
    10021 cgcactagcg aagatatcaa cgatgcgctg aattatcgca ccgtaacgaa aaacattatt
    10081 cagcatgtag agaataaccg tttctctttg ttggaaaaat taactcagga tgtgctcgat
    10141 atcgcacgtg aacatcactg ggtgacgtat gctgaagtgg agatcgataa actgcacgcg
    10201 ctgcgctacg ccgattcggt atccatgacc ttaagctggc agcgttaacc gccatatcgg
    10261 gaggctgcat gaacatagtg atcaccggag ggacgggatt aattggtcgc catttgattc
    10321 cgcgtttgct ggagctgggc catcaaatca cggtagtgac gcgtaaccca cagaaagcca
    10381 gttccgttct cggccctcgg gtgacgctat ggcaagggct agccgatcaa agcaacctca
    10441 acggcgttga tgcggtaatc aacctggccg gagaaccgat tgctgataaa cgctggactc
    10501 acgagcaaaa agagcgtctc tgccaaagcc gctggaatat cacgcaaaaa ctggtcgatt
    10561 tgattaatgc cagcgacacg ccgccgtcgg tactcatttc cggctcggca acgggctatt
    10621 atggcgactt aggtgaagtg gtggttaccg aagaggaacc gccgcataac gaatttactc
    10681 ataaactctg cgcccgctgg gaagagattg cctgccgggc gcaaagtgac aaaacgcgag
    10741 tgtgcctgct gcgaaccggc gtggtactgg caccggacgg cggtattctc ggtaaaatgc
    10801 tgccaccgtt tcgtcttggc ctgggcgggc cgattgactc tggtcggcag tatctggcct
    10861 ggattcatat tgatgatatg gtcaacggta ttctctggct gctggataac gagctgcgcg
    10921 ggccatttaa tatggtttcg ccctacccgg tacgcaatga acaatttgcc catgcgctcg
    10981 gtcacgcgct gcatcgcccg gccattttgc gcgtccccgc gaccgccatt cgactgttaa
    11041 tgggcgaatc ttcagtactg gtattaggtg gacaacgcgc gctgcctaaa cggctggaag
    11101 aagcgggttt tgcgtttcgc tggtacgatt tagaagaggc gctggcggat gtcgttcgct
    11161 gatgtggttt acagcaaaca tccgccagtt aactcccggt gttacaggat tagtggcttt
    11221 gcgcgataag atcgtctggt gaaagtcggg tcaccatcat aactaactct ctgtctaaac
    11281 ctctatccag catctcctga gcaatacgca gggcttcttc gtgtttgccc tgcattgcgc
    11341 cttcttcacg taatctgtca gcaatggtca tcagtttctc cttttcttgt ggtgcgcgtt
    11401 ccgctatctc accaataaat gcacgaaaac gctgggcatc ccctgtttgt aatacgtaat
    11461 taaacagggc ttttagctgt ctgtcattag tgttccctgt aactagcagc gaaacaattt
    11521 ggtcgactaa tcccaacaga tcgcgctgac gaatatgttt ctgaattaac tccaacagcg
    11581 ccattttgcg gtgttgcata atctcgtcat ccggcaccac ggtaatatcc accaacggaa
    11641 acgctgatga atatattttg cgggctatag caggctcggc aaattcatca agccagcaga
    11701 gtgaataagg ataaggactt ctgcaaccat gataaaacag catcgggatc accaatggaa
    11761 gctctttata gcccg
//
LOCUS       DADLFA010000130        11689 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000130 DADLFA010000000
VERSION     DADLFA010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11689
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.130"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(68..715)
                     /gene="ychE"
                     /locus_tag="JQH65_002948"
     CDS             complement(68..715)
                     /gene="ychE"
                     /locus_tag="JQH65_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HAY6140304.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            1193..3868
                     /gene="adhE"
                     /locus_tag="JQH65_002949"
                     /gene_synonym="adhC"
     CDS             1193..3868
                     /gene="adhE"
                     /locus_tag="JQH65_002949"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAY6140305.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGILSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            3992..4888
                     /locus_tag="JQH65_002950"
                     /pseudo
     CDS             3992..4888
                     /locus_tag="JQH65_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(5170..5787)
                     /gene="tdk"
                     /locus_tag="JQH65_002951"
     CDS             complement(5170..5787)
                     /gene="tdk"
                     /locus_tag="JQH65_002951"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAY6140306.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            6392..6805
                     /gene="hns"
                     /locus_tag="JQH65_002952"
     CDS             6392..6805
                     /gene="hns"
                     /locus_tag="JQH65_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HAY6140307.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(6949..7857)
                     /gene="galU"
                     /locus_tag="JQH65_002953"
     CDS             complement(6949..7857)
                     /gene="galU"
                     /locus_tag="JQH65_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAY6140308.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEQVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(8059..9072)
                     /gene="rssB"
                     /locus_tag="JQH65_002954"
     CDS             complement(8059..9072)
                     /gene="rssB"
                     /locus_tag="JQH65_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HAY6140309.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDATVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(9164..10069)
                     /gene="rssA"
                     /locus_tag="JQH65_002955"
     CDS             complement(9164..10069)
                     /gene="rssA"
                     /locus_tag="JQH65_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HAY6140310.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQCREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGESLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            10182..10640
                     /locus_tag="JQH65_002956"
     CDS             10182..10640
                     /locus_tag="JQH65_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HAY6140311.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            10690..11532
                     /gene="purU"
                     /locus_tag="JQH65_002957"
     CDS             10690..11532
                     /gene="purU"
                     /locus_tag="JQH65_002957"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HAY6140312.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVNHEGLSRNEHDQKMADAIDAYK
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
BASE COUNT         3160 a         2916 c         2649 g         2964 t
ORIGIN      
        1 gcgggaacaa agtaaaaata atgacgaagt aaacggagca tggcagctcc gtttcatttg
       61 aaaggaatta attaagcagg ccggggaaaa tccccttaat accagtaacg ataaattcaa
      121 tccccaatgc catcagcaat agccccataa tacgcgtaat cacgttgatg ccggtctggc
      181 gtaaaacccg taccagccaa ggtgccatgc ggaacaatcc ccagcaacat aaagcgaaca
      241 atgcaatagc cacaaagaaa ccaaacagat agctaatgct gtgataacgc gtaccccaga
      301 cgatggtaga actgatcgcc cctggccccg ccatcaacgg caacgccaat ggcaccacac
      361 caatgctttc acgtaccgcg gtttctgatt tttcttgctt gttctgttta tcctcgccaa
      421 gcttgccgct gatcatcgac atcgctatcg tcaccaccag gatgcccccg gcgatacgga
      481 acgaatcaat tgatatacca aaaagttgca gaatcgtgtc gccgagaaaa agcgagatcc
      541 acaagataat ggccacagac aggttggctg taaggttagt tttgtttcgc gctgccgctg
      601 tctgataact ggtcatgctg ataaagacgg gaataatccc taccgggttg accagcgcaa
      661 ataacccgat gaaaaatttg aagtaaacgg gaaaatcaaa aaaggtctga atcacggtta
      721 gctccgaagc aaaagccgga taatgttagc cataaataag gttgaaaaga cgcgctgaca
      781 atacgccttt tgacagcatt tttcacctcc taactactta aaattgctat cattcgttat
      841 tgttatctag ttgtgcaaaa catgctaatg tagccaccaa atcatactac aatttattaa
      901 ctgttagcta taatggcgaa aagcgatgct gaaaggtgtc agctttgcaa aaaatttgat
      961 ttggatcacg taatcagtac ccagaagtga gtaatcttgc ttacgccacc tggaagtgac
     1021 gcattagaga taataactct aatgtttaaa ctcttttagt aaatcacagt gagtgtgagc
     1081 gcgagtaagc ttttgatttt cataggttaa gcaaatcatc accgcactga ctatactctc
     1141 gtattcgagc agatgattta ctaaaaaagt ttaacattat caggagagca ttatggctgt
     1201 tactaatgtc gctgaactta acgcactcgt agagcgtgta aagaaagccc agcgtgaata
     1261 tgccagtttc actcaagagc aagtagacaa aatcttccgc gccgccgctc tggctgctgc
     1321 agatgctcga atcccactcg cgaaaatggc cgttgccgaa tccggcatgg gtatcgtcga
     1381 agataaagtg atcaaaaacc actttgcttc tgaatatatc tacaacgcct ataaagatga
     1441 aaaaacctgt ggtattctgt ctgaagacga cacttttggt accatcacta tcgctgaacc
     1501 aatcggtatt atttgcggta tcgttccgac cactaacccg acttcaactg ctatcttcaa
     1561 atcgctgatc agtctgaaga cccgtaacgc cattatcttc tccccgcacc cgcgtgcaaa
     1621 agatgccacc aacaaagcgg ctgatatcgt tctgcaggct gctatcgctg ccggtgctcc
     1681 gaaagatctg atcggctgga tcgatcaacc ttctgttgaa ctgtctaacg cactgatgca
     1741 ccacccagac atcaacctga tcctcgcgac tggtggtccg ggcatggtta aagccgcata
     1801 cagctccggt aaaccagcta tcggtgtagg cgcgggcaac actccagttg ttatcgatga
     1861 aactgctgat atcaaacgtg cagttgcatc tgtactgatg tccaaaacct tcgacaacgg
     1921 cgtaatctgt gcttctgaac agtctgttgt tgttgttgac tctgtttatg acgctgtacg
     1981 tgaacgtttt gcaacccacg gcggctatct gttgcagggt aaagagctga aagctgttca
     2041 ggatgttatc ctgaaaaacg gtgcgctgaa cgcggctatc gttggtcagc cagcctataa
     2101 aattgctgaa ctggcaggct tctctgtacc agaaaacacc aagattctga tcggtgaagt
     2161 gaccgttgtt gatgaaagcg aaccgttcgc acatgaaaaa ctgtccccga ctctggcaat
     2221 gtaccgcgct aaagatttcg aagacgcggt agaaaaagca gagaaactgg ttgctatggg
     2281 cggtatcggt catacctctt gcctgtacac tgaccaggat aaccaaccgg ctcgcgtttc
     2341 ttacttcggt cagaaaatga aaacggctcg tatcctgatt aacaccccag cgtctcaggg
     2401 tggtatcggt gacctgtata acttcaaact cgcaccttcc ctgactctgg gttgtggttc
     2461 ttggggtggt aactccatct ctgaaaacgt tggtccgaaa cacctgatca acaagaaaac
     2521 cgttgctaag cgagctgaaa acatgttgtg gcacaaactt ccgaaatcta tctacttccg
     2581 ccgtggctcc ctgccaatcg cgctggatga agtgattact gatggccaca aacgtgcgct
     2641 catcgtgact gaccgcttcc tgttcaacaa tggttatgct gatcagatca cttccgtact
     2701 gaaagcagca ggcgttgaaa ctgaagtctt cttcgaagta gaagcggacc cgaccctgag
     2761 catcgttcgt aaaggtgcag aactggcaaa ctccttcaaa ccagacgtga ttatcgcgct
     2821 gggtggtggt tccccgatgg acgccgcgaa gatcatgtgg gttatgtacg aacatccgga
     2881 aactcacttc gaagagctgg cgctgcgctt tatggatatc cgtaaacgta tctacaagtt
     2941 cccgaaaatg ggcgtgaaag cgaaaatgat cgctgtcacc accacttctg gtacaggttc
     3001 tgaagtcact ccgtttgcgg ttgtaactga cgacgctact ggtcagaaat atccgctggc
     3061 agactatgcg ctgactccgg atatggcgat tgtcgacgcc aacctggtta tggacatgcc
     3121 gaagtccctg tgtgctttcg gtggtctgga cgcagtaact cacgccatgg aagcttatgt
     3181 ttctgtactg gcatctgagt tctctgatgg tcaggctctg caggcactga aactgctgaa
     3241 agaatatctg ccagcgtcct accacgaagg gtctaaaaat ccggtagcgc gtgaacgtgt
     3301 tcacagtgca gcgactatcg cgggtatcgc gtttgcgaac gccttcctgg gtgtatgtca
     3361 ctcaatggcg cacaaactgg gttcccagtt ccatattccg cacggtctgg caaacgccct
     3421 gctgatttgt aacgttattc gctacaacgc gaacgacaac ccgaccaagc agactgcatt
     3481 cagccagtat gaccgtccgc aggctcgccg tcgttatgct gaaattgctg accacctggg
     3541 tctgagcgca ccgggcgacc gtactgctgc taagatcgag aaactgctgg catggctgga
     3601 aacgctgaaa gctgaactgg gtattccgaa atctatccgt gaagctggcg ttcaggaagc
     3661 agacttcctg gcgaacgtgg ataaactgtc tgaagatgca ttcgatgacc agtgcaccgg
     3721 cgctaacccg cgttacccgc tgatctccga gctgaaacag attctgctgg atacctacta
     3781 cggtcgtgat tatgtagaag gtgaaactgc agcgaagaaa gaagctgctc cggctaaagc
     3841 tgagaaaaaa gcgaaaaaat ccgcttaatc agtagcgctg tctggcaaca taaacggccc
     3901 cttctgggca atgccgatca gttaaggatt agttgaccga tccttaaact gaggcactat
     3961 aacggcttcc acaacaggga gccgttttct tatgccactt ctcaatgatc tgctcgattt
     4021 cagtgaccat ccgcttatgc ctccgccctc tgcacaacta tttgcagaac accttcccac
     4081 cgagtggata caacactgcc tgacgctttc tgctcatgcg accgttcgcc gccgtcgttt
     4141 accgggggac atggttatct ggatggtggt gcaatgagcc aattaccgat gttgttcgcc
     4201 gtctgaacct gagcgcggat ggcgaagcgg ggatgaacct gctggcccgc agcgctgtca
     4261 cccaagcgcg tcagcgcgtg ggggccgccc cagtggaatg gctcttccgc cagaccgcac
     4321 agacaggggc gcggaacgtt acctgaagga tgactggcac ggcctgcaac tttttgccat
     4381 tgatggcgca cagttcagga cacctgataa acccgagctg cgtgaatatt atgggtctgc
     4441 caacacatcc actaagcggc agaacgccta cccggttatg cgtctggtgg ccttaatgaa
     4501 cctgggaagt cacattctgc tgaatgccgt gaccgcacct taccggcaga gcgaaactgt
     4561 gctggcccac tccatgctcg ccaccatccc ggataactct attacgttgt ttgataagct
     4621 tttttacagc gaagacctgc tgctaacgct gaaccagaag ggttgtaacc gccactggct
     4681 actgcctgcg tggaagaata tcgcatcaga aatgatagag ctgggaaaca cggcgtcacc
     4741 agggaccatc ccgaaacgac tggagcatct tcggggtgct ctggaagtgg tgttcataac
     4801 aaaacgcccc cgaccatcaa ggccgaggtc ggttaagata tcaaaaaccc gttatcctgt
     4861 gaaacatagt gccgctcctc ttaagtgaac ggcattgccc ttctggggcc gtattttttg
     4921 tttacccaaa gcaacttttc cataaaccga cagcattagc cttcatcata tttgcgacga
     4981 tgtataacgc ctaaacacag ggatattgta ctttacaggt cacaagtcaa cgtcggtgct
     5041 taagagccct gtgaggcgta tagcggcgtt aaaaaactgc cgagaagggt atatagcccg
     5101 gaagaagtgc gtaaaacgaa ctgacaggat aaaagtgccc tgctcaccct gtcagtaaag
     5161 aaattcttat taatcgtggc gatgcctttc ctgaatagcc gttaatgagc cgacttgtaa
     5221 cgcctcttta tagtgtttac ggcatacaga aacgtatcgt tcattaccac caattaccac
     5281 ctgctcacct tcgttataag gtctgcctgc ttgatcaaga cgcagcacca tgcttgcttt
     5341 acggccacaa aaacagatgg tttttaattc aaccagtttg tcggaccatg ccagtaagta
     5401 ttggctgcca ataaataatt cacctcgaaa atcggtacgt aaaccataac aaagtacggg
     5461 tatatcgagt tgatcgacaa cctccgataa ttcatatact tgttgtctgg ttaaaaactg
     5521 gcattcatca accagtacgc aatgaattgc ctgctgttca tgttccgcac gaatctcatc
     5581 aaataatgat gaattttggt taaataattt tgcaggcgat gacaaaccta tacgcgaact
     5641 gactttcccg gcaccaaagc gatcatcaat ttctgccgta tatacgacag tgcgcatgcc
     5701 gcgttcttgg taattgtatg aagattgcaa caatgctgta gacttacccg cattcattgc
     5761 ggaatagtag aaatatagct gtgccatcag ccacaggccc tcaatgatat gaaataaagt
     5821 tggctggagt ttatcataat tcgtcagttt ttcagctcaa cagtatgcac agaagtgggt
     5881 atatgcgttc tcccttacga agccttgcat aatccttctg agctatcatt acaactgcct
     5941 cgcttgttat aagcgggtaa atgactgctg gtaactattc acaatcttta acctgttgcg
     6001 caagtaatag ccctctgttg acctccagga gatagtgcaa tactaagtcc atgctcttat
     6061 tgcgactgtt ctacttttca tcattcgctt aatagggaat tctcgtaaac acaactaata
     6121 cagaagactg aaaggccgtc agcctacgat aatctcccca taaaatgtga catgaatcag
     6181 gaagttttaa cctcacgtgc tgcgaaatca tcggtgtaaa tagggctata tgccgcgtct
     6241 tttctggcta attttatgaa aagatattta ttggcggcac aaaataaaga acaattttga
     6301 attccttaca ttcctggcta ttgcacaact gaatttaagg ctctattatt acctcaacaa
     6361 accaccccaa tataagtttg agattactac aatgagcgaa gcacttaaaa ttctgaacaa
     6421 catccgtact cttcgtgcgc aggcaagaga atgtacactt gaaacgctgg aagaaatgct
     6481 ggaaaaatta gaagttgttg ttaacgaacg tcgcgaagaa gaaagcgcgg ctgctgctga
     6541 agttgaagag cgcactcgta aactgcagca atatcgcgaa atgctgatcg ctgacggtat
     6601 tgacccgaac gaactgctga atagccttgc tgccgttaaa tccggcacca aagctaaacg
     6661 tgctcagcgt ccggcaaaat atagctatgt tgacgaaaac ggcgaaacta aaacctggac
     6721 tggccagggc cgtactccag ctgtaatcaa aaaagcaatg gatgagcaag gtaaatccct
     6781 cgacgatttc ctgatcaagc aataatcttt tgtagattgc aattgctaaa aatcccgcca
     6841 tcggcgggat tttttattgt ccggtttaag acaatttaat aagaataagc tataaaaaaa
     6901 cggcgtcgat tgttcaacgc cgtttcgtgg ataacaccga tatggatgtt acttcttaat
     6961 gcccatctct tcttcaagcc aggctttaaa ttccgtgcca agggtgttat gacgaatacc
     7021 gtattcaacg aaggcctgca tgtaacctaa tttattaccg cagtcatggc tcttcccttt
     7081 catatgatag gcttccaccg tttctttttc gatcagcata tcaattgcgt cggtgagctg
     7141 aatttcatca ccagctcccg gaggggtttt tgccagcaac ggccaaatat ccgcgctaag
     7201 tacgtaacga cccacaatag cgagattaga cggcgcaaca tccgctttcg gtttttctac
     7261 cacaccaacc atcggtacac tttcacccgg cgctaattca acgcctttgc aatccacaac
     7321 gccatatgcg gtcacatcag caacctgttc aaccatgatc tggctatgac ccgtttcatc
     7381 aaagcggcgg atcatctctg ccaggttatc ctgtgacaaa tcggattcat attcatccag
     7441 aataacatca ggcaaaataa cagctaccgg ttcatcaccc actaccgggt gagcacacaa
     7501 taccgcgtgt cccaggcctt tcgccagccc ctgacgaact tgcataatag tcacgtgcgg
     7561 cggacaaata gactgcactt catcaagcag ttgacgtttt acacgttttt ccagcattgc
     7621 ttccagttca aaactggtat caaagtggtt ttcaatcgag tttttagatg agtgcgtaac
     7681 cagcacaatt tcagtaatgc cagccgcaat acattcattc acgacgtatt gaattaatgg
     7741 cttatcgaca agtggcagca tctctttcgg gatggctttc gtcgccggca acatcctggt
     7801 tcctaatccc gcaacgggga taacggcttt tttgactttc gtattaatgg cagccattta
     7861 aattctcctg gactgttcgt gttttgaacg tgttcatatt tctgtatcgc atcccagtat
     7921 atcagcaccc cgaaggggct aagttctgaa aaggaggcga gttagcataa ttaagacaat
     7981 tacttatacg aatggcgacg atagtaccac cagccgacat tagcaggtaa tgcaaattta
     8041 gtccgcgtta tcgtttgctc attctgcaga caacatcaag cgcagtcgac caccggttcc
     8101 ccatatttgg cattgccagg catcgcatcg ctggctcaat tgattcaaat aagcgttacc
     8161 taaagtgcct aacggaacac cattactgat ttgcacctgg tgttcgccgg tatttaacgt
     8221 cgcattcaga cccgcagaaa ccagaatgag atttttcagt tcgcgatgat aatagccaac
     8281 taatagcgga aactgccccg gcagattggc ctgacggagt aaatggttta cctgcttcaa
     8341 taacgcgccc agctctggca accgttgatt ttggtgtgca agctgttcct gtaataatcc
     8401 gttaaacaat gcgcgtaata acaaggcagc aagtacgcca ttatgtccag ctcgggtgac
     8461 atcaaggcaa taaaatgcca gatcgttttc cgaaagtgcg gcaatatcaa gcaccaggcc
     8521 gggtttgtcc gcggcaacca attgacgata attaacccgg caatgggaaa tcacctgctg
     8581 aaccggcggt tgtagttcct gtaataattt cgccgctgcg gcagggttat caaccgttgc
     8641 atcccaatcg cgaaaaagcc tttcctcttc ctcaacgcgc gaattaaaca tgctgggata
     8701 gagacaggca aaaaccatct cgcgcaagcg attcagatct ttaactggtt tcagcaaaac
     8761 atcttcaacg cccagacgta acgctttggc aatatctgcc atattttcag tggcagatat
     8821 caccagaact ggggtctggt cgcctctgtt acgtatatgc tccagcagtt taagcccgtt
     8881 cattcgtggc atcgcgatat cacatatcat caggtctgga gtgaaacctc ccagcaactc
     8941 aagggcatcc accccatcag ccgccagtac cgttgtcgct cccaatgagg aaaaccatga
     9001 atccagaagc gagcgaaata cctgctcatc ttcaacaatg agaatctgtt ttcctaccaa
     9061 tggctgcgtc atgttctctc ccctgactgg ctttactcaa tagtggcatg ctattgccac
     9121 ctgcgcctgt cagaattaac ttaagtgtag ataaaaattc tggtcaaatg ttggtgcgta
     9181 ccaacggcaa aagttcgtcc attttccttt ccactgccag ctgtccggcc gcaatggcag
     9241 cgtgcgcgcg atggaaatca agcgtagata tttgcgggca aacaggttga atcagaatat
     9301 cgggcggatc acctgccatg cggttccttt taaggcggtt ctccagcacc tggatagaag
     9361 tggtcataat ctctgttgcc gttggcgctg tcaccgcccg acgtgttgtt atactgccta
     9421 acctttcttt cagacgcgca tgccacggca aagattcacc attctcgcta ttttcttcac
     9481 tgacattaaa ggagagcaaa tcttgttgca tcaaatgagc atcgtgctgc aggtcgaccg
     9541 ctatcacaat atcagccccc aatgcacgcg tgagggaaat aggaattggg ttaactactg
     9601 ctccatcaac cagccagtag ccgttatgtg caacaggtgc catgagtcct ggaatactgc
     9661 atgatgcgcg aatagcaaga tggagatcgc cttcagtaaa ccataattca cgtcccgtac
     9721 ttaaattggt ggcaacagcc gcaaagcgac gggaacaatt ttcgatctct gtttccggca
     9781 ttatttcgcg gcattgattg aagacacgct cgccgcgcag taacccaccg cgctgccagg
     9841 agagatccat caggcgtaaa acatcccaat aactgaaaga ggtcacccaa tcttccagcg
     9901 cagataatcg atcgcatgca taggcagcgc ccaccagcga accaattgaa catcctgcaa
     9961 cgatatcaat ttcaataccc acttttttta gcgcattaat aacgccaata tgcgaccaac
    10021 ctctcgccgc gccagatccc agcgccagcc ctatttttat ctttctcatt attcccgcaa
    10081 gattcccctg gaatgcaatc gtagccacat cgctactgct cggttaacat agtgctactc
    10141 aggcggttaa tacgccgtta ttgtttccag ggagattatt tgtgtctcag ctttgtccct
    10201 gtggtagtgc tgtcgagtat agcctatgtt gccaccctta tgtgtctggt gaaaaggttg
    10261 cacctgatcc agaacatctc atgcgttcgc gttactgcgc ttttgtgatg caagacgcag
    10321 attatttaat aaagacctgg catccctctt gtggagcagc ggcgttacgt gccgaattga
    10381 tggccggatt tgcgcatact gaatggttgg gattgaccgt atttgaacat tgttggcagg
    10441 acgccgacaa tatcggattt gtcagttttg ttgctcgatt taccgaaggt ggcaaaacag
    10501 gtgcaataat tgaacgttcg cgatttttga aagaaaacgg tcaatggtac tatattgacg
    10561 gtacacgtcc gcagtttggt cgcaacgatc cctgcccttg tggttcaggt aaaaaattta
    10621 aaaagtgctg cggccaataa tggttgacgg tacggtttag caaacactct caacaaggtt
    10681 tttccagcaa tgcattcact ccaacgtaaa gttctgcgta ctatttgtcc ggaccaaaaa
    10741 ggtctgatcg cacgtattac caatatttgc tacaagcacg agttaaatat cgtacagaac
    10801 aatgaatttg ttgatcaccg taccgggcgc ttttttatgc gcacggaact ggaagggatt
    10861 tttaatgatt ccaccctgct ggcggatctc gatagcgcat tgccagaagg ctccgtgcgt
    10921 gagctgaatc ctgccggtcg tcgccggata gtgattctgg tcactaaaga agcgcattgc
    10981 cttggcgatt tgttgatgaa agccaattac ggcggcctgg atgtcgaaat cgcggcagtt
    11041 attggtaacc acgatacttt acgttctctg gttgagcgtt ttgatattcc gtttgagctg
    11101 gtaaaccatg aagggttaag ccgcaacgag cacgatcaaa agatggcaga cgccattgat
    11161 gcttataaac ctgactatgt ggtgctggcg aagtatatgc gggtattaac gccggaattt
    11221 gtggcacgct tcccgaataa aatcatcaat attcaccatt cattcctgcc tgcgtttatc
    11281 ggcgcacgtc cttatcacca ggcctatgaa cgtggtgtga agattattgg cgcaaccgct
    11341 cactatgtga atgacaatct ggacgaaggc ccaatcatca tgcaggacgt tattcatgtc
    11401 gatcatacct acacagctga agatatgatg cgcgcaggtc gtgacgtcga gaaaaacgtc
    11461 ttaagtcgtg cgctatacaa agtactggca cagcgcgtct ttgtttacgg taatcggacg
    11521 attattcttt aatcgccagc aaaaataact ggttaccttt aatccgttac ggatgaaaat
    11581 tacgcaacca gttcattttt ctcaacgtaa cactttacag cggcgcgtca tttgatatga
    11641 tgcgccccgc ttcccgataa gggagcaggc cagtaaaaag cattacccc
//
LOCUS       DADLFA010000131        11545 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000131 DADLFA010000000
VERSION     DADLFA010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11545
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.131"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..156
                     /locus_tag="JQH65_002958"
     CDS             <1..156
                     /locus_tag="JQH65_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY6140313.1"
                     /translation="ILQLCRNDSLMIGRESFSVDRITPDDGGCSYIRLRREGAGNVRA
                     GGYYEGA"
     gene            143..730
                     /locus_tag="JQH65_002959"
     CDS             143..730
                     /locus_tag="JQH65_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001214489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY6140314.1"
                     /translation="MRGLENAIRNLNSLDTRMVPQASAWAVNRVAAKVVSVATRQVAR
                     NTVAGDNQVKGGPLKLVRQRVRVFKASPAGKMYARIRVNRGNLPAIKLGTTCRRAGDG
                     LKVGKYFFRGAFIQQLANGRWHVLRRLPEACFETGKDSKGRPRKNRLPVEVVKIPLAG
                     PLTQAFEDARDRIIAAEMPKQLGYALKQQLRIHLG"
     gene            727..1128
                     /locus_tag="JQH65_002960"
     CDS             727..1128
                     /locus_tag="JQH65_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY6140315.1"
                     /translation="MNRHTQIRQAVLASLKGECGGNVVLFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFVRAQAPDSELDTWMENTIFPAMKDIPVLSGLID
                     TMIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            1139..1879
                     /locus_tag="JQH65_002961"
     CDS             1139..1879
                     /locus_tag="JQH65_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY6140316.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGKGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTLATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTITSKEVMTRTVKISGVGRPYLAEEGAETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            1938..2324
                     /locus_tag="JQH65_002962"
     CDS             1938..2324
                     /locus_tag="JQH65_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HAY6140317.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            2333..2661
                     /locus_tag="JQH65_002963"
                     /pseudo
     CDS             2333..2661
                     /locus_tag="JQH65_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
     gene            2633..5674
                     /locus_tag="JQH65_002964"
     CDS             2633..5674
                     /locus_tag="JQH65_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAY6140318.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAAGDAERSSARMQ
                     RFMERQTQAARQTMHAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGVGQQ
                     DYLALISEITAKTRALTQAEEQATRQKAAFIRQLKEQATRQNLSSSELLRARAAQLGV
                     SSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGNFGALRGSGITLANRA
                     GWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEEFNRQLALTGHYAGVTVG
                     QLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNALHFLTATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     KRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIDALQEKIQRAKKYP
                     WTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTRATRNDEATRLLLQYS
                     QQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLARKN
                     ELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERALREQHNLDIATAGMGD
                     KQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEEVLKGSLKRQLNENKR
                     YWQELEVAQGDWKNGAMRAFQNFTADADNAVGTAEQMFTVAFSSAGNALATFCTTGKL
                     NFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGVYQSADLSRYSGTVVN
                     RPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSGMVMFAPQYNIGINND
                     GTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            5674..6003
                     /locus_tag="JQH65_002965"
     CDS             5674..6003
                     /locus_tag="JQH65_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY6140319.1"
                     /translation="METFRWKVRPDMNVVSEPKVVAVKLGDGYEQRRPAGLNNLLSTY
                     SVTIRVRKGEHPPLKAFLERHGGVRAFQWTPPYDWKPIRVACRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            6003..6702
                     /locus_tag="JQH65_002966"
                     /pseudo
     CDS             6003..6702
                     /locus_tag="JQH65_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
     gene            6707..7450
                     /locus_tag="JQH65_002967"
     CDS             6707..7450
                     /locus_tag="JQH65_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY6140320.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGERYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            7387..7989
                     /locus_tag="JQH65_002968"
     CDS             7387..7989
                     /locus_tag="JQH65_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HAY6140321.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            8050..11529
                     /locus_tag="JQH65_002969"
     CDS             8050..11529
                     /locus_tag="JQH65_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HAY6140322.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVENTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCR
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDAQAILRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSCTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITAVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDDAEGYLSFYKGLINNTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCSLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKATAGITIS"
BASE COUNT         2709 a         2731 c         3713 g         2392 t
ORIGIN      
        1 attttacagc tgtgtcgcaa tgattcgctg atgattggcc gggagtcttt cagcgtggat
       61 cgtattactc ctgatgacgg cggatgtagt tatatccggt tgcggcgtga aggagcagga
      121 aacgtaaggg cagggggata ttatgagggg gcttgagaat gccatccgca atctgaacag
      181 tcttgatacc cgtatggtgc cgcaggccag cgcatgggca gtaaaccggg ttgctgcaaa
      241 agttgtctcg gttgccaccc ggcaggtcgc caggaatacc gttgcgggag ataaccaggt
      301 gaaggggggc cccctgaaac tggtgcgtca gcgcgtccgg gtatttaaag ccagtccggc
      361 aggaaaaatg tatgcccgta tccgtgttaa ccggggcaat ctgcccgcca ttaagcttgg
      421 cacaacatgc cggcgtgccg gtgacggact gaaggtgggg aaatactttt ttcgtggtgc
      481 gtttatccag cagctggcga acggtcgctg gcatgttctc aggcgtcttc ctgaagcgtg
      541 ttttgaaaca ggaaaggaca gtaaggggcg gcccagaaaa aatcgtctgc ccgtggaggt
      601 tgtgaaaatt ccgctggccg gaccgctgac acaggcattt gaggatgccc gcgatcgcat
      661 cattgccgcg gaaatgccga aacagctggg gtatgccctg aaacagcaac tgaggataca
      721 tttgggatga acaggcacac acaaatccgc caggcagtac tggcaagcct gaagggcgaa
      781 tgcgggggga atgtcgtgct ttttgacggg cttccggcat ttattgatgc gcaggaactg
      841 cccgccgtgg cggtgtggct gagtgatgcg cagtacaccg gaaaaatgac ggatgaggac
      901 gactggcagg ctgttctgca tattgccgtt ttcgtccgtg cacaggcgcc ggattctgag
      961 ctggatacgt ggatggagaa caccatattt ccggccatga aggatattcc ggtgctttcc
     1021 ggactcatcg acaccatgat cccactcggt tttaactatc aacgtgataa tgagatggcc
     1081 acctgggcga tggcggaaat cacgtaccag atcacgtaca cgaattaagg aggtggcaat
     1141 gaccacacca aatccactgg caaagacgaa aggtgcggga acgacgttct ggatgtacac
     1201 cggcaagggc gatgcgtttg cgaacccttt gtcggacact gactggctgc gtcttgcgat
     1261 ggtgaaggat ctgcaacctg gcgaaatgac cgctgatgca gaagatgaca cttatctcga
     1321 tgatgaagat gcagactgga aaacgacaac ccaggggcag aaatccgtcg gtgatacttt
     1381 ggcgacgctg gcctggcgtc cgggtgacag cgggcagaaa aaactggttc agttgttcga
     1441 ttccggtgaa gtctgcgcgt ttcgtatcaa ataccccaac ggtacggttg atgttttccg
     1501 tggctggctg agttcactgg gtaaaaccat tacctcaaaa gaggtgatga cccgtactgt
     1561 aaaaatcagc ggtgtggggc gtccgtatct ggcagaggag ggcgctgaaa ccgtgggtgt
     1621 taccggactg acggtggcac cggcatctgt cagtgtcaaa gcgggagcaa ccaccacact
     1681 gacctttaca gtaaaacctg acggggccag tgacaaagcg atcagtgtgc attcgtcaga
     1741 tcctcagact gcttcggtga ccctgagcgg gcttgtggcc acggtgaaag gcgtgaagca
     1801 gggcagtgtc agcatcgtgg gcatgacctc tgacggagag tttgtggcag tggctgcggt
     1861 gaccgtcagc gcaccataac aggacgatac tcatcattgc cccggttatc cggggctttt
     1921 tgcatccgga gaacatgatg tttctgaaac aggatacgtt taattatgaa aaacagtccg
     1981 tggtgctcag tgagctgtcc gggctgcaga gaattgaata tctgacgttt gttcagcagc
     2041 gaacggcaaa gtttgatgca caggagggag aactgccgga ggctgaacga cagattgctt
     2101 ttctgcgtat gggaatggat atcaatgcct ggctggtttc ccgctcactg tggaatgctg
     2161 agcagtctca ggatgttgag acgctttgcg catccattat gacaacatgg tcgtatgatg
     2221 cgctgggcgc gggggcggag agggttctgt cgctgagcgg tatggggacc attgagaatg
     2281 ccggggatga tgatcatgag gcgctgacgc cggaaaagtc ctgacgcggg aaatgcagtt
     2341 tgtcatgcgg cttgcccggg agttccggcg ggcagactgg cggcggatgc tgtcggaaat
     2401 gtcggccact gagcttggtg agggggcgat tatttccgga tgcagagctt cagtgatgtg
     2461 tggatggatg cgcagtttgc ctcgctgaag gcattgatcg tgagaatggt gtccggcagc
     2521 agtgatgctg cggtggctga tttcagcctt ttaccggaag agaacgggat accggagcga
     2581 acggacgaag aactgatgca tcttggggaa ggtatttccg gaggtgtgcg ttatggacca
     2641 gatagccaac ctggtcattg atttggggat tgatgcggca gagtttaaaa atgaaattcc
     2701 ccgtatcaaa aaccttctga atggtgcagc cggtgatgca gaacggtctt ctgcccgtat
     2761 gcagcgtttt atggagcgtc agactcaggc ggcccggcag acaatgcatg cggcttcttc
     2821 ggctgcaaca gcagcatcag cccatgcgca gacggtggag aagaatgcac gggctcatga
     2881 acgcatggcc cgtgaggtgg aacaaacccg tctgcgcgtg gatgccctga atcagaaaat
     2941 gcgcgaggaa caggcgcagg ccagggcact ggcggaggcg caggataaag cggctgccgc
     3001 cttttatcgc cagattgaca gtgtgaaaca ggccggtgcg gggcttcagg aattacagcg
     3061 tattcagcag cagatccgac aggccagaaa cagtggcggg gttggtcagc aggattatct
     3121 ggcgctgatt tcggagatca cggcgaaaac ccgtgccctg acgcaggcag aggaacaggc
     3181 cacccggcag aaagcggcgt ttatccgcca gcttaaagag caggcaaccc gccagaatct
     3241 gtcgtcttct gagttgcttc gcgccagggc ggctcagctg ggggtaagca gtgctgcaga
     3301 agtgtatatc cgcaaaatgg agcgggcagg aaaagccaca cattcgctgg ggctgaaaag
     3361 tgcggcagcc cggcgggagc tgggggtgtt aatcagtcag atggcgcgcg gcaattttgg
     3421 tgcgctgagg ggatccggga taacgctggc taaccgtgcc ggatggacag gcgcactgat
     3481 gtcgccgaaa ggcatgatga ctggcggcgt tattggcgga cttgtcgcgg cggtcctggg
     3541 tctgggtaaa gcctggcatg acggccggaa ggagggcgag gaatttaacc gtcagctggc
     3601 gctgacggga cattatgccg gtgtcactgt cgggcagttg tggaaactca gccgggccat
     3661 atccgggaat ggtatcacgc aacatgcggc agccggtgcg ctggcacagg tggtgggcag
     3721 tggggcattt catggaaacg atattggtat ggtggcgaaa gctgccgcac agatggagcg
     3781 atcggttggc cagtcggtca gcgataccat aaatcagttt aagcggctga aggatgatcc
     3841 tgtaaatgcc gcgaaggcgc tggacaatgc gctgcatttt ttgactgcca ctcagcttga
     3901 gcagatacgc gtccttgggg agcaggggcg gtccagtgat gcggcccgga tcgccatgtc
     3961 tgcgctggca gaggaaaccg gtaaacgcac atctgatatt gataataatc tcaatgcgct
     4021 gggtagtacg ctgcaaacct tgtctgactg gtggaagcag ttctgggatg cggccatgaa
     4081 tattggtcgt gaagactcgc tggatgcgca gattgatgcg ttacaggaaa aaattcagcg
     4141 cgcgaaaaaa tatccgtgga caaacgcctc cacacaggtg gagtacgatc agcagcgtct
     4201 taacgatctt caggagaaaa aacgccggaa ggatttgcag gatgcaaaag cgcaggcaga
     4261 acggaattac caggagcaac agaaacgccg gaatgctgaa aatgccgcgc tgaaccggat
     4321 gaatgaaacg gaagctgcac gacatcagcg ggaaattgcg cgtattaatg ccatgcagta
     4381 cgccgaccag gctgtcaggg atgcggcgat acagcgtgaa aatgaacgtt acgaaaaagc
     4441 cattaagaaa aatacacggg caacccgtaa tgatgaggcc acccggttat tgctgcagta
     4501 cagtcagcag caggcacagg tggaagggca gattgctgct gccagacagt cagcaggcat
     4561 tgccactgaa aggatgactg aagcgcataa acagcttctg gccctgcagc agcgcatcag
     4621 cgacctggac gggaaaaagc tgacggcaga tgaaaagagt gtgctggccc gtaagaatga
     4681 gctgattcag gcgctgacgc tgctggatgt gaaacagcag gagctgcaga aacagacagc
     4741 gcttaacgac ctgagaaaaa aaacggttca gctgaccagc cagctggcag acaaagaacg
     4801 tgcactgcgt gagcagcaca atctggatat tgccactgca ggtatggggg ataagcagcg
     4861 gcagcgctac caggcacagt tgcgcatccg gcaggaatac cggcaacagt tgcaacagct
     4921 tgagaatgac agtcgccaga aaggcactta cgggacggag gactaccgga gggctgagga
     4981 ggtgctgaag gggagcctga agcgacaact gaatgaaaac aaacgctact ggcaggaact
     5041 ggaagtggcg cagggcgact ggaaaaacgg tgccatgcgg gcgtttcaga attttacggc
     5101 ggatgcggat aatgcggtgg gaacggcaga acagatgttt acagtggcat tcagcagtgc
     5161 cggtaatgca ctggcgacat tctgtaccac cggtaagctg aattttaaat ccttcacctc
     5221 ttccctgttg tcagatatgg ccagaattat ggcacagatg gccatgatgc aggcggtaaa
     5281 gggcgtcggc tctttattcg gcttcacgac taatgctgat ggcggtgttt accagtctgc
     5341 tgatttgagt cgctacagtg gcacggtggt taaccgtccg acgttttttg cttttgcaaa
     5401 aggcgcgggt gtgatggggg aagcgggacc tgaagccatt ctgccactgc gtcgcggtgc
     5461 tgatggtaag ctgggggttg tggcggatac tggtggttca ggcatggtga tgtttgcccc
     5521 gcagtacaac atcgggatca ataacgacgg cacgaacggg cagataggtc cggcagcact
     5581 gaaggtggtt tatgacctcg ggaaaaaagc ggccgcggac tttatgcaac agcaggcccg
     5641 tgatggtggc cggttaagtg gagcataccg gtaatggaga cgtttcgctg gaaggtgcgc
     5701 ccggatatga atgtggtatc agagccaaaa gtggtggcag tgaagctggg tgatggttat
     5761 gaacagcgtc gcccggcggg actgaataat ctgctgtcga cttacagcgt gacgatacgt
     5821 gttcgtaaag gtgaacaccc acctttaaaa gcctttctgg aacggcacgg tggcgtccgc
     5881 gcatttcagt ggacgccacc ttatgactgg aaaccgatca gggtggcttg tcgtaaatgg
     5941 tcggcaagcg tgggggcgtt atgggtgact gtaacggccg attttgaaca ggtggtgaac
     6001 tgatgcagga tatccggcag gaaacactga atgaatgcac ccgtgcggag cagtcggcca
     6061 gcgtggtgct ctgggaaatc gacctgacag aggtcggtgg agaacgttat tttttctgta
     6121 atgagcagaa cgaaaaaggt gagccggtca cctggcaggg gcgacagtat cagccgtatc
     6181 ccattcaggg ggagtggttt tgaactgaat ggcaaaggca ccagtacgcg ccccacgctg
     6241 acggtttcta acctgtacgg tatggtcgcc gggatggcgg aagatctgca gagtctggtc
     6301 ggcggaacgg tggtccggcg taaggtttac gcccgttttc tggatgcggt gaacttcgtc
     6361 aacggaaaca gtgacgccga tccggagcag gaggtgatca gccgctggcg catcgagcag
     6421 tgcagcgaac tgagcgcggt cagtgcctct tttgtgctgg ccacaccaac ggagacggat
     6481 ggcgcggttt tcccggggcg tatcatgctg gcgaatacct gtatgtggac ctaccgttct
     6541 gatgagtgtg gttacacggg cagggcagtg gctgacgagt tcgacaaacc aacgacggat
     6601 atccggaagg acaaatgcag caagtgtatg cgcgggtgtg agttgcgcaa caataccggt
     6661 aatttcggcg gtttcctttc catcaataaa ctttctcagt aaatccatga cacagacaga
     6721 atcagcgatt ctggcgcacg cccggcgatg tgcgccagcg gagtcgtgtg gcttcgtggt
     6781 gagaacgccg gagggggagc ggtatatccc ttgtgtgaat atttctgcag agccggaggc
     6841 gtattttcgt atcgcaccgg aagactggct gcgggcagag atgcaggggg agattgtggc
     6901 actggtccac agtcatcccg gtggtctgcc ctggctgagt gaggctgacc ggcggctgca
     6961 ggtgcagagt gatttgccgt ggtggctggt ctgccggggg gcgattcaca agttccgctg
     7021 tgtgccgcat ctcaccgggc ggcgctttga gcacggggtg acggactgtt acacgctgtt
     7081 ccgggatgct tatcatctgg cggggattga gatgccggat tttcatcgcg gggatgactg
     7141 gtggcgtcac ggtcagaatc tctatctgga taatctggag gccacagggc tgtatcaggt
     7201 gccgttgtca tcagcacaac cgggcgatgt gctgctgtgc tgttttggtt catcggtgcc
     7261 gaatcatgcc gccatttact gcggcgacgg tgagctgctg caccatattc ctgaacaact
     7321 gagtaaacga gagaggtata ccgacaaatg gcagcgacgc acacactccc tctggcgtca
     7381 ccgggaatgg cacgcatctg cctttacggg gatttgcaac gatttggccg ccgcatcgac
     7441 cttcgtgtga aaacgggggc tgaagccatc cgcgcactgg ccacacagct cccggcgttt
     7501 cgtcagaaac tgagtgacgg ctggtatcag gtgcgcattg ccgggcgtga tgcaggtgaa
     7561 accgaattgt ctgcccgtct taatgagccg ctggcaaatg gtgcagtgat ccacatagta
     7621 ccgcgtctgg tgggagctaa aagtggcggt gtgtttcagg cggtgctggg ggcagctgtt
     7681 atggcggttg ctatatggat gccgggggta ggaattatgg cgagtaatct gctgttttct
     7741 ctcggtgcca gtatgacgct tggcggtgtt gcacagatgc tggcaccgaa agccagaact
     7801 ccccgtacac agacaacgga taacggcaaa cagaacacct gtttctcctc actggataac
     7861 atggttgccc agggcaatgt tctgcctgtt ctgtacggtg aaatgcgcgt ggggtcgcgg
     7921 gtggtatctc aggagatcag cacggcagac gaaggggatg gtggtcaggt tgtggtgatt
     7981 ggtcgctgat gcaaaatgtt tcatgtgaaa ccgcctgcgg gcggttttgt cgtttatgga
     8041 gcgtgaggaa tgggtaaagg cagcagtaag gggcataccc cgcgcgaagc gaaggacaac
     8101 ctgaaatcca cgcagttgct gagtgtgatt gatgccatca gcgaagggcc ggttgacggt
     8161 ccggtggatg gattaaaaag cgtgctgctg aacggtacgc cagtcctgga cagcgagggg
     8221 aagacaaact tttccggtgt tacggtggtg ttccgcgccg gcgagcagga gcagacaccg
     8281 ccggaggggt ttgaatcttc cggctcagag actgtgctgg gtacggaagt gaaatacgac
     8341 acgccgatca cccggaccat cacgtcggca aacattgatc gtctgcgcct gaccttcggt
     8401 gtgcaggcac tggtggaaaa cacctcaaag ggggaccgga atccgtcgga agtccgcctg
     8461 ctggttcaga tacagcgtaa cggtggctgg gtgacggaaa aagacatcac cattaagggc
     8521 aaaaccactt cgcagtatct ggcctcggtg gtggtgggta acctgccgcc gcgcccgttt
     8581 aatatccgga tgcgcaggat gacaccggac agcaccacag accagctgca gaacaaaacg
     8641 ctctggtcgt cgtacaccga aatcatcgat gtgaaacagt gctacccgaa cacggcgctg
     8701 gtcggcgtgc aggtggattc agagcagttc ggtaaccagc aggtgagtcg caattatcat
     8761 cttcgcgggc gcattctgca ggtgccgtcg aactataacc cgcagacgcg gcaatacagc
     8821 ggtatctggg acggaacgct taagccagca tacagcaaca acatggcctg gtgtcggtgg
     8881 gacatgctca ctcatccgcg ctacggcatg gggaaacgtc ttggtgcggc agatgtggac
     8941 aaatgggcgc tgtatgtcat cggccagtac tgcgaccagt cagtgccgga cggatttggc
     9001 ggcacggagc cgcgcatcac ctgtaacgct tacctgacca cacagcgtaa ggcgtgggat
     9061 gttctcagtg atttctgctc ggcgatgcgc tgtatgccgg tatggaacgg gcagacgctg
     9121 acgttcgtgc aggaccgacc gtcggataaa gtgtggacct ataaccgcag taatgtggtg
     9181 atgccggatg atggcgcgcc gttccgctac agcttcagcg ccctgaagga ccgccataat
     9241 gtcgttgagg tgaactggat tgatccggac aacggtcatg agacggcgac agagcttgtg
     9301 gaggacgcgc aggccattct ccgttacggt cgtaacgtca cgaagatgga tgcctttggc
     9361 tgtaccagcc ggggacaggc gcaccgcgcc gggctgtggc tgattaaaac ggaactgctg
     9421 gaaacgcaga ccgtggactt cagcgtcggc gcagaagggc ttcgccatgt accgggtgat
     9481 gtcattgaaa tctgcgatga tgattatgcc ggtatcagca ccggtggtcg cgtactggcg
     9541 gtgaacagcc agacccggac gctgacgctc gaccgtgaaa tcacgctgcc gtcctcctgt
     9601 accacgctga taagcctggt tgacggaaat ggcaatccgg tcagcgtgga ggttcagtcc
     9661 gtcaccgacg gcgtgaaggt gaaagtgagc cgggttcctg acggcgttgc agaatacagc
     9721 gtgtgggggc tgaagctgcc gacgctgcgc cagcgcctgt tccgctgcgt gagtatccgt
     9781 gagaacgacg acggcacgta tgccatcacc gccgtgcagc atgtgccgga aaaagaggcc
     9841 atcgtggata acggggcaca ctttgacggt gaccggcgcg gcacggtgaa tggtgtcacg
     9901 ccgccagcgg tgcagcatct gaccgccgaa gtcaccgcag acagcgggga atatcaggtg
     9961 ctggcgcgat gggacacgcc gaaggtggtg aagggtgtga gcttcctgct tcgcctgacc
    10021 gtggcagcgg acgacggcag tgagcggctg gtcagcacag ccaggacgac ggaaaccaca
    10081 taccgcttca ggcagctggc gctggggaat tacagtctga cagtccgggc ggtaaatgcc
    10141 cgggggcagc agggcgatcc ggcgtcggta tcgttccgga ttgcggcacc ggcagcgcct
    10201 gtcactattg aactgatacc ggggtatttt cagataacgg cggtcccgaa actggctgta
    10261 tacgacccga cggtgcagtt tgagttctgg ttttcggaaa agcggattgc ggatatcagg
    10321 caggttgaaa ccagcgcccg ctatcttggc acggcgctgt actggatagc cgccagtatc
    10381 aatatcaggc cgggccatga ttattatttt tacgttcgca gtgtgaacac cgttggcaaa
    10441 tcggcatttg tggaggccgt cggtcgggcg agcgatgatg cggaaggtta tctgagtttt
    10501 tataaagggt tgatcaataa cacgcatctc ggcaaggagt tgtggacgca gattgataac
    10561 ggtcagcttg cgccggacct gactgaaatc aggacgtcca ttacgaatgt cagcaatgaa
    10621 atcacgcaaa ccgtcaataa aaaactggaa aatcagagtg ctgcaatcca gcagatacag
    10681 aaagttcagg ttgatacaaa taataacctg aacagcatgt gggctgtgaa gctgcaacag
    10741 atgaaggacg gacgccttta tattgcgggt atcggtgccg gtattgagaa tacgccagca
    10801 ggaatgcaga gtcaggtgct gctggcggca gacaggattg cgatgattaa tcctgcgaat
    10861 ggcaacacaa agccgatgtt tgttggtcag ggcgatcaga tattcatgaa cgacgtgttc
    10921 ctgaaacgcc tgacggctcc gaccattacc agcggcggta atcctccggc attttccctg
    10981 acacctggcg gacggctgac ggcgaaaaat gccgatatca gcggtaacgt gaacgcgaac
    11041 tccgggacgc tcaacaacgt cacgattaat aagaactgtc gggttctggg aaaattgtcc
    11101 gcgaaccaga ttgaaggcga tctcgttaaa acagtgggca aacctttccc acgggactcc
    11161 cgggcaccgg agaggtggcc atcagggacc attaccgtca gggtttatga cgatcagccg
    11221 tttgatcggc aaattgttat tcccgcggtg gcgtttcgcg gtgctaaaca tgagcggaag
    11281 aataacaata tttattcgtc atgcagcctg atagtgaaga aaaacggtgc tgaaatttat
    11341 aaccgaacga ccctggataa tacgctgata tatacgggtg ttattgatat gcctgccggt
    11401 cacggtcaca tgacgctgga gttttctgta tcggcatggc tggtaaatgg ctggtatccc
    11461 acagcaagta tcagcgattt gctggttgtt gtgatgaaga aagccactgc aggcatcacg
    11521 attagctgaa ttttataacc cagat
//
LOCUS       DADLFA010000132        11405 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000132 DADLFA010000000
VERSION     DADLFA010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11405
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.132"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..921
                     /gene="gap"
                     /locus_tag="JQH65_002970"
     CDS             <1..921
                     /gene="gap"
                     /locus_tag="JQH65_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAY6140323.1"
                     /translation="DVVAINDLTSPKILAYLLKHDSNYGPFPWSVDFTEDSLIVDGKS
                     IAVYAEKEAKNIPWKAKGAEIIVECTGFYTSAEKSQAHLDAGAKKVLISAPAGEMKTI
                     VYNVNDDTLDGNDTIVSVASCTTNCLAPMAKALHDSFGIEVGTMTTIHAYTGTQSLVD
                     GPRGKDLRASRAAAENIIPHTTGAAKAIGLEIPELSGKLKGHAQRVPVKTGSVTELVS
                     ILGKKATAEEVNNALKQATTNNESFGYTDEEIVSSDIIGSHFGSVFDATQTEITAVGD
                     LQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(963..2403)
                     /gene="aldA"
                     /locus_tag="JQH65_002971"
                     /pseudo
     CDS             complement(963..2403)
                     /gene="aldA"
                     /locus_tag="JQH65_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
     gene            complement(2558..3400)
                     /locus_tag="JQH65_002972"
     CDS             complement(2558..3400)
                     /locus_tag="JQH65_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAY6140324.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALFNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLRQKLPVCEPLV
                     CKRA"
     gene            complement(3672..7574)
                     /gene="hrpA"
                     /locus_tag="JQH65_002973"
     CDS             complement(3672..7574)
                     /gene="hrpA"
                     /locus_tag="JQH65_002973"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HAY6140325.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSGTIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            7775..8380
                     /gene="azoR"
                     /locus_tag="JQH65_002974"
     CDS             7775..8380
                     /gene="azoR"
                     /locus_tag="JQH65_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAY6140326.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(8431..9747)
                     /locus_tag="JQH65_002975"
     CDS             complement(8431..9747)
                     /locus_tag="JQH65_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HAY6140327.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWPFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAER
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGSCIVLMELMVMIQLWWPVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKITAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVDEAISYIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(9737..11405)
                     /locus_tag="JQH65_002976"
                     /pseudo
     CDS             complement(9737..11405)
                     /locus_tag="JQH65_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415928.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
BASE COUNT         2843 a         3097 c         2713 g         2752 t
ORIGIN      
        1 gacgttgtcg ctattaatga tctcacttcc ccaaaaattc tcgcctacct gctgaaacat
       61 gattcaaact acggaccatt cccctggagc gttgatttta cggaagattc acttatcgtt
      121 gatgggaaaa gtatcgcggt ttacgccgaa aaagaggcta aaaatattcc gtggaaagcg
      181 aaaggtgcag aaatcattgt cgaatgtact ggcttttata cctccgccga gaaatcgcag
      241 gcgcatcttg atgctggtgc gaagaaggtg ttgatttccg cccctgccgg tgaaatgaaa
      301 actatcgttt ataacgtcaa tgacgacact ctggatggca acgacaccat tgtttccgtg
      361 gcgtcatgca ccactaactg tcttgcgccg atggccaaag ccttgcatga cagtttcggg
      421 atagaagtcg gcacgatgac gaccattcat gcctatactg gcacccagtc actggtggat
      481 ggcccacgtg gtaaagattt acgtgcttca cgcgcagcgg cagaaaatat cattccccac
      541 actacggggg cggcaaaagc cattggtctg gagatcccgg aactgagcgg caaactgaaa
      601 ggtcatgcgc aacgcgtgcc ggtgaaaaca ggttcggtca ctgaactggt atcgattctc
      661 ggaaaaaaag cgactgccga agaggtgaat aacgcactta aacaagcaac caccaataac
      721 gagtcatttg gttataccga tgaagaaata gtctcttccg atatcattgg cagccatttc
      781 ggttcggtgt ttgatgccac gcaaacggaa attaccgccg tgggcgattt acaactggtg
      841 aaaacggtcg cctggtacga taacgaatat ggcttcgtca cgcagcttat tcgcaccctc
      901 gaaaaattcg ctaaactctg acgcgcacag gcggaggaaa aaacctccgc ctcttttact
      961 cattacgact gtaaataaac cacctgggtc tgcagatatt catgcaggcc atgtttacca
     1021 tctgcgccgc caataccgga tttacgccat ccggcgtgga agccttgcat agcttcgaag
     1081 ttttcacggt tgatgtaagt ttcaccaaac ttcagccctt taatggcttt catcgcgacg
     1141 ttcagatttt gggtatagat ttgatgaggt caggccgtaa tcactgtcat tagccattga
     1201 gatagcctct tccagcgtgt caaatgcgac caccggcaga accgggccaa aggtttcctc
     1261 atgcataatc gacatttcct ggcgaacatc cagcagcaat gtcggcggat aataatatcc
     1321 tttcccctct accgctttgc cacccaacgc cactctcgcc ccttcttcta ctgcgcgcgc
     1381 cactttttgc tcaacccttt ccaacgccgc ggcgttaatc aacggcccca tcgcaatgtc
     1441 gttgcgttca gcggggttac caaattgaac cgcctgcatc gcttcaccca gccgattgac
     1501 gaactgatca taaatgcctt tctgtacata aacacgttct gcacagttac acacttgccc
     1561 actattaatg acgcgtgaat caacgatggc tttgactgcc agttcaagat cggcatcgtc
     1621 cattacgata gctggtgctt taccccccaa ttccagacac actttggtga tgtttttcgc
     1681 cgcagtcgcc atgatcttct cacctgcaga gacgctgcct gtcatactga ccattgcgac
     1741 ctttgggtta cccgccagtt cttgcccaac ggtttcacca cgccccagta caaggttaaa
     1801 cacgccgcgc ggaaggccta tttcatcgac gattttggcg aatgcaatcg cattgtttgg
     1861 cgtaaattca ctaggtttaa tgacgatggt attaccggtc aaaagagcgg gagccatttt
     1921 gcgggcaatg aggaagaacg ggaagttcca cggcagaatg ccggtagtca caccaagcgc
     1981 acgtttaaac aaaagaatat tttctcctgg acgatcgctt tgaataatct cgccctcgta
     2041 acgccgtgcc cactccgcca tgtaatcgat atagtcggca gtaaaagcca cttcgacttc
     2101 agccagctgc tggatcttgc ccccttcttc aacaatcagc gcactgattt cactggcgcg
     2161 ttcgcggatc ccggcggaga ttttgcgcaa ccaactggcg cgttcaatag caggcaacgc
     2221 ttcccattct ggttgtgcac gttctgctgc atcgattgcc ttacgggcat cctcggcctg
     2281 accatcgggt atgcgggaaa tgacagcctc tgtagcaggg tttaccacat caatccatgc
     2341 gtctccacgc caggtaacaa actgtccatc gatatacata ggatgttgaa cgggtactga
     2401 catgggcgac tcctgtgatt tatatgtttg ttcttggtaa atacattgtt aattaacatc
     2461 aatcatcata gagacggcat cgtgaaagga caattctgtg aagggcttca taaaaacaag
     2521 tgtaaaagga ataaaaggca gaaataaaag tcaggagtca tgcccgtttg caaacaagtg
     2581 gttcacaaac gggcaatttt tgacgtaacg agcgactttc catcgcctcg atgagcaccg
     2641 catcgtgttt aagcgtttgc catgcatgga tgacttctgc cggaaaatca acgtgaacga
     2701 taaaatctcg cccgcgggga ccgtagccat cgctatcatc gcgtaaacgc ggcagctcgc
     2761 cagtgagtag tgagagataa cgctcaaccg gccataatcc ttgtaactga ttaataaaga
     2821 ttacactgtc tgcgttattt cctaactgag gaacgaatcc gggataactt aaccagcgtg
     2881 gtgcatcggg attgtcccca gtcatacggc ggaacgtcgc catcgtgcgc cgctgcatgg
     2941 tggggtcctg aacaacgata gccgtatgaa ctcgttctac ggcctgatta aatagcgcga
     3001 tgctaaagcg tgcgttttca ccgcagtttg ttgactggtc ttcaatccag attttttcat
     3061 gcggaatgtg ccagaactga tgagcgatat ccgccaggat ggtcgcttct gctctgccag
     3121 tggtgcggat agtgttgtag tgcggatgct gtgcgatggc gctatacaaa aaagttgtcg
     3181 agtgaccgat accaccacta atcagtaaag gaatttgctg atcgcgagca atcttacatg
     3241 ccgcgtcgat agtcggcata accgcattgc ctgcaaggat cacgcaatcg gcctgatacg
     3301 gcacctcacc ggagaaatca tcctgcgcca gccactgtcc gataacattt atggcatcta
     3361 tagttgccgg cgaaagcgtc ggaaacgggg taatgttcat ggttcctcct tatcgttccc
     3421 ttaagaatac tgcgtgagat aatagtgaga agctgagcac tctgaaagga ggaagtcgta
     3481 tgtccttaac cgccactccg caagcctgac atatctttta aggaaatgct gtagataaac
     3541 attattcatc ttgcagcaga gaattgtatt tttctgaatt cctgaatttt tacccttacc
     3601 gttttagaat taataaaaaa cccggcgcta aatgcttacc gggttttaga tttattaggc
     3661 aaatagcagg gttaaccgct aatctgctcc atcgcctgca aaatacgctt atctgaaatc
     3721 ggataaggcg taccaagttg ttgagcgaag taactaacgc gcagctcttc tatcatccaa
     3781 cggatctctt tcacgtcttc atcctcacga cgtgcaggcg gcagtttgtt gatccattgc
     3841 tgccacgcct gctggacgtt ttcgactttc agcatctgtg cacgatcgcg atgtggatca
     3901 accgccagtt tttccagtcg tttttcaatc gcctgcaaat atcgcagcgt gtcgcccagc
     3961 cgtttgaagc cgttaccagt gacaaaaccg cgatatacca acccgcccat ctgcgcttta
     4021 atgtcagaaa gccccagtgc catggtcata tccacccgcc ctttcagacg tttgttgata
     4081 ttgaacactg ccgtaagaat ttgctcgacc tgcttcgcaa tatctaccac cgtgtcgttc
     4141 agttcggcac gcactttctc atgcagcgca gcaaagcctt cttccgtcca gaccgggcca
     4201 ccattggcgt cgatcagttg atccacaccg caggagatac agtcgtcgat cagctccagc
     4261 actttgccat acgggttgaa gtacagtccc agcttggctt tgttcggtaa cttttcatgt
     4321 aaatacttga tcggcgaggg aatatttagc agcagtagac ggcgaagacc attccacatt
     4381 gcctgttttt gctccagcgg gttatcaaac agtttgatcg ccacgctgtc gcgttcatcc
     4441 accagcgccg gccacgcctt cactttatag ttgccacgct tctgttcgta actttccggc
     4501 agctgaccaa aactccagat atgtaagccg ctctgctcga taccgtcatc cgccaccgcg
     4561 gacagcgttt cctgcacttt gcctttcagc gcatctttca gatcttgtag cgagcgccct
     4621 tcttttagct tcttgttttt gtcatccacc acgcggaagg tgattttcag gtgatcgggc
     4681 acctgatccc agtgccagtc ttcgcggtca acggtaacgc cggtcatccg ccgtaactcg
     4741 cgctcaaggc tgtcgagcaa cggtaactcc agcggtgtga cgcggcctaa aaacgcttcg
     4801 gcgtagtttg gtgcaggcac aaaattacgg cgtaccggtt tcggcaacga tttaatcaga
     4861 gcaatcacca attcgcggcg cagaccgggg atctgccatt caaacccgct ttcctcaacc
     4921 tggttaagta acggcagcgg aatatgtacg gtcacaccgt ccgcgtctgc gcctggctca
     4981 aactgatagc tcaaacgcag cttgagattg ccctgatgcc agaagttcgg gtaatccagc
     5041 ttgctgattt tttccgcccc ttctttgatc aacatgctct tttcaaagtt gagcaaatca
     5101 ggcgtttcgc ggctgacttt tttccaccag ctatcgaagt gacgagcgga gattacatcg
     5161 tggctgatgc gctggtcgta gaactcaaac agcgtttcgt catcaaccag aatatcgcgg
     5221 cgacgtgatt tgtgttccag ctcttccact tcggcccgca gtttcaggtt ttcacggaag
     5281 aatgcgtgac gcgtctgcca gtcaccttcc accagcgcat ggcgaataaa gagttcacga
     5341 cataacgccg gatcgatctg gctgtagttg accttgcgcg cggcaacaat cggcaaacca
     5401 taaacagtga ctttttccgt tgccatcacc gcgccctgcg cccgttccca gtgcggttcg
     5461 ctgtaggtac gtttaatcaa atgctgagcg acaggctcca cccattccgg gtcgatacgc
     5521 gcagcaatgc gcccccacag gcggctggtt tctaccagtt ccgccaccat tacccatttc
     5581 ggcggttttt tgaataaacc agaaccgggg aagatggaga aacgcgcgtt acgtgcgccg
     5641 gtatattctt gtttatcggc atctttcatg ccgatatggg aaagtaaacc cgtcagcaac
     5701 gcaatgtgaa tttcgcgata ctccgccggt tcgctgttaa ccggaatgcc aagttctttc
     5761 accacctgac gcaactgggt gtagatatcc tgccattcgc gcacgcgcag atagttgaga
     5821 taatcggtac gacacaggcg acggaaggcg tttgaagaaa gcgccttttg ctgctcgcca
     5881 agataattcc acagatttac aaacgcgagg aagtcggatt ctttgtcgtg gaagcgacga
     5941 tgtttttcgt ccgatgcctg ctgtttgtcc atcggccgct cacgcgggtc ctgaatggag
     6001 agcgcggacg tgataatcat cgcctcacgc acgcagccat gtttctgcgc ttccagcacc
     6061 atacgcgcca gacgaggatc gaccggcagt tgcgagagct ggcgaccgag cggcgtcagt
     6121 ttataggcgc tggcctgttc atccgtggtg atcgcgccca gttcttcgag cagacgcacg
     6181 ccatcctgga tattgcgttt atccggtgct tcgacaaacg ggaacgcagc gatatcgccc
     6241 agccccagcg cggtcatctg caaaataacc gaggccaggt tggtacgcag aatctccgga
     6301 tcggtaaact ccgggcgcga gaggaaatcg tcttcggaat agagacgaat acagatccct
     6361 tcggacacac gaccacaacg gcctttacgc tgattggcag acgcctgtga aatgggttca
     6421 atcggcaaac gctgcacttt ggtgcgatag ctgtagcggc tgatacgcgc cgtaccgggg
     6481 tcgataacgt atttaatccc cggtacagtc agcgaggttt ccgcgacgtt ggtcgccagc
     6541 acaatgcgtc gcccgctgtg cgactggaac acgcggttct gttcgctatt cgaaagccgc
     6601 gcataaagcg gcaagatttc ggtatggcgt aagttcagct tgttcagcgc atcggcggta
     6661 tcgcgaattt cccgctcgcc gctcataaag atcagaatgt cgccagggct ttcctggctc
     6721 agttcgtcta cggcgtcaaa aatcgcctgc aactgatcgc gctcggtgtc atcggcttct
     6781 tcaacaatcg ggcgatagcg cacttccacc ggataggtcc gaccggagac ttcgataatc
     6841 ggcgcattat taaagtggcg agaaaagcgt tccgggtcga tagtcccgga agtgataatg
     6901 atttttaggt caggacgccg cggcagcaac tctttcaaat agccgagcag aaaatcgata
     6961 ttcaggctgc gttcgtgtgc ttcgtcaata atgatggtgt cgtactgcat cagcaggcgg
     7021 tcttgctgga tctccgccag caggataccg tcggtcatca gcttgaccat cgtgttatca
     7081 cttacgtgat cgctgaaacg cactttgtaa ccgatgcaac cgcctggctc cgttttcagc
     7141 tcttccgcaa tacggttcgc caccgttctt gccgccagac gacgcggctg ggtatggccg
     7201 atcagtcctt taatcccgcg ccccagctcc atacagattt tcggtagctg ggtcgtttta
     7261 ccggaaccgg tttccccggc gacgatcacc acctggtgat cacgaatcgc ttcgagaata
     7321 tcctgttttt tctgactaac tggcaaattg tccggataag tgatttccgg tcgtgccgct
     7381 tcacgcagca ggactttccc tgccgcctgg tcaatctctt tcgccatctc ctggaaaatg
     7441 gcctgttgtg catcaggatt tttaaccttc ttaacaccat gcagacggcg agaaaaacgc
     7501 agtctgtcgc gcagcatcaa cgaatccagc cgctgctgca aagcagtaaa ggtcaatttt
     7561 tgttgttctg tcataacgtt agtgggcagt agtctgcctg attaattttc ttttaaatga
     7621 tagatataga gtaccacatc gcggctttct atgcgttgtt caataaattc gaacataggc
     7681 ttcgatttat tgcgctatct ctgtggcagg attgtgaata aagtgtcagc aagcaacggg
     7741 gcatcgccaa ttcaaacatc tataaggaaa caccatgagc aaggtattag ttcttaaatc
     7801 cagcatcctg gcagggtact ctcagtctaa tcagttgtcc gattattttg ttgaacaatg
     7861 gcgcgaaaag cactccgctg atgaaatcac cgttcgcgac ctggctgcaa atccgattcc
     7921 ggtactggat ggcgaactgg ttggcgctct gcgtccgagc gatgcgccgc tgactccgcg
     7981 tcagcaggaa gctctggcgc tttccgatga gctgattgcc gagctgaaag cccacgacgt
     8041 tatcgttatt gcggcaccga tgtataactt caacatctca actcagttga aaaactattt
     8101 tgacctggtt gcacgcgcag gcgttacttt ccgctatacc gagaacggtc cggaaggtct
     8161 ggtaacgggt aaaaaagcca tcgttattac cagccgcggt gggatccaca aagatggacc
     8221 aacggacctg gtgacgccgt atctgtccac gttcctcggc tttatcggca ttaccgatgt
     8281 gaaatttgtc ttcgccgaag ggatcgcata cggtccggaa atggcagcga aagcgcagtc
     8341 tgacgcgaaa gcggccatcg acagcattgt tgctgcataa ccggacacga tcttgatgtg
     8401 gactgtagtt cagtccacat ctcaatccac ttacctgttt tcccataatt tcagcatcgc
     8461 tttgtgatct tccttaagca caatatgcga acgtctggct cgaatataac taatcgcttc
     8521 atcaacggtt ttacagtgtc cgtaacataa caaccatgcc gccactacca gcgcactgcg
     8581 cgataatccc aacgcgcaat ggaccagaac gctgccttgc tcttcgcgta atgtttccag
     8641 catcgccacg gcctgtcgga gctccccctc ttccggaacc accagatcca gcatcggtac
     8701 acagaaatag agtcgatctt ttgtcgctcg tccccgaggg aattcaaagg tgacgtccag
     8761 aaccgcattc tgtgccggaa tatgtcgtgg aaacgcccct aaataaacac cagcagtaat
     8821 tttgctcacc ggctccaggc gacgacaaaa ccaacgcata gacagccaca tcccgatgcg
     8881 ccagggcaat gtcagccagt aaacggcggg cgggagtttc ccctgactat ctttccctgt
     8941 tgttatcgcg ccaagcccac cgtacccacg gccaataatg agtagcgata atactggcca
     9001 acataaccag actggccacc ataactgaat catcaccatt agctccatca acacaatgca
     9061 cgagcccgcg cctacgacat atggcagtgc tattttgatt cgacgttgat caggtttctg
     9121 ataattccaa cgacgatcga cgggcaccat ccagtcaatc aacataccta ccgccagccc
     9181 tgtgatgaca tcaataaaat gatgctgcca ggtcgtcaga gtagaaatag cgatgagtaa
     9241 aaaccatccg ccacagactt tacgccacct ctcagccaga tgctgacgaa agtgacgcca
     9301 aagtagccag cagagaataa tatgcagcga aggagactgg ttataaggca gatcaaacag
     9361 ttcaagttgc gaaaatggcc atcccgtcac cccactcact tcaggacgga taaaactaaa
     9421 cttcagcgga tagagcaaaa aaccgcagca ggccattacc gttgccagaa taagccggtg
     9481 gacaagtcgg cgctgttcga atgtcgtgct acaaacgaac agcgaaaatc catataaaag
     9541 atccagactc cagtaaggaa caatagtcca gggaagaaaa gggatcgccg tttcccaacc
     9601 gaatacctga ctggggatat catggctgtt aaggtcctga accgcggtga actgattaag
     9661 agatccatag gtgaagaaaa aaaacggggc cagcaacaat aaccagccag cgccttgtag
     9721 caatacgttc cgacgttcag ttatcacgac gcaccgccat cgaaaccgta aaaatacccc
     9781 actcatcaat ccgttgtgtg catttatcaa atccggcatc acgcacgagt gaatccatct
     9841 ccccttgcga acgcacgcgc attacccacg gtttaccatc tttatgactg gttaacaccc
     9901 cggcaatcat ctccagttga gggtgccacg gctgcccggt gtagatgaga atgccgaccg
     9961 gttcgatggc attggcaaga cctgcgagtg agttttttac ctgctcattt tcgggaaaaa
    10021 gctcatacag tccagagaca attgccagcg taggccgcgg agttaacgcg ctgagttcct
    10081 ccgggttaaa cgcatcgccc tgttcaaaac gcacccgccc agacattccc cgttgggcaa
    10141 tcatctcttg cccctgtgca acatttaact cgctgtaatc acgtaacaaa atatcgctta
    10201 cggcaggctc gtttgccagc gcatccagta catagcgccc atgccctgcg gcaatgtcaa
    10261 ccacgcggac ggctaaacct ttggcgtgga gatcggcaac ggcttgttta atcagtattt
    10321 gtaaatgggt tttgcgctgg cgaataccgc gccagccaac actgttcagg tagattttgt
    10381 cgattaagcg cccgaatgcg ttactacctt gcggttgatt ttgatagaca taatccagcg
    10441 aactgcctga atcaaagccg gtgctcattc cgagatgcag gcccgcagag tgcgtcccga
    10501 acaatttcat cgcctttcgc ataaagcgat acgctaaatc aaccggcgat aatagcacgg
    10561 gtccaccaga aagtagccgc cattgatccg ctgatggtcc tgtgcagtct tcatgctgat
    10621 aatcaaattt ttgcgatttg ttagcatata aacgactaat aaagctttgc attttttcaa
    10681 atgccagcgc cctgttctct tcacccaacg tgtcgtgata aaagcctggc agcagatgca
    10741 gctctttcag agggctacgt aatctctgat aaaaatcaat ctgcggttgg cgatgcacca
    10801 catagtcatc acctgatatc agaagttgcg tggggagcgt aatcgccgcc gcatctctaa
    10861 taatacgttc agacgtttta tagagatcga gcaagatatt gacggcaatc gcccgtgtga
    10921 tcagcggatc attattgaaa ctcgccaccc gttgccgatc gtgggtcaaa tagcgtcctt
    10981 tcacatagga attaataaaa aacagaccac gcagacgatg ccataacgcc agcgcaggac
    11041 gtgccagcgg cacatacaat ttaaccttaa aggccggaga agccagcacc agcccgcgaa
    11101 ttgcaggcgc ataatcatga acctatgtgg caaccagcac tgcgccgacg ctttgcgcga
    11161 tcacaaccac ctcttccagt ccaacctagc tgtcgctggc agcaaaacgg acaaattcat
    11221 cgacatcctg cactgaacgc gcaagagatg ggctataacc acgcggcccc gaagtttttc
    11281 catgcccccg agcatcccat gcataaaaag cagtatctgg catcgccagt tcatcaacga
    11341 tatgttgtag acgaccagaa tgttcatggc cacgatgaaa taagacaatg acctttttcg
    11401 cccct
//
LOCUS       DADLFA010000133        11321 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000133 DADLFA010000000
VERSION     DADLFA010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11321
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.133"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..1072
                     /locus_tag="JQH65_002977"
     CDS             <1..1072
                     /locus_tag="JQH65_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAY6140328.1"
                     /translation="IEESSDDGDEYDYSPAKEEWVITAANAKPQCDIIHEAWQSRAVI
                     RYDMAVPLNLSERSARQSTGRVGVVLVVTLSHNSRRIDVDINLDNQADDHRLRVLVPT
                     PFNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMAV
                     FSEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLS
                     CRLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMP
                     PVGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEEN
                     QGSNLSGPFLPGQSRTFSYRLA"
     gene            1920..3488
                     /gene="cydA"
                     /locus_tag="JQH65_002978"
     CDS             1920..3488
                     /gene="cydA"
                     /locus_tag="JQH65_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HAY6140329.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            3504..4643
                     /gene="cydB"
                     /locus_tag="JQH65_002979"
     CDS             3504..4643
                     /gene="cydB"
                     /locus_tag="JQH65_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HAY6140330.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            4658..4771
                     /gene="cydX"
                     /locus_tag="JQH65_002980"
     CDS             4658..4771
                     /gene="cydX"
                     /locus_tag="JQH65_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HAY6140331.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            4771..5064
                     /gene="ybgE"
                     /locus_tag="JQH65_002981"
     CDS             4771..5064
                     /gene="ybgE"
                     /locus_tag="JQH65_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HAY6140332.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIIGLIFFFF"
     gene            5214..5618
                     /gene="ybgC"
                     /locus_tag="JQH65_002982"
     CDS             5214..5618
                     /gene="ybgC"
                     /locus_tag="JQH65_002982"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HAY6140333.1"
                     /translation="MNTTLFRWPARVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            5615..6307
                     /gene="tolQ"
                     /locus_tag="JQH65_002983"
     CDS             5615..6307
                     /gene="tolQ"
                     /locus_tag="JQH65_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HAY6140334.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            6311..6739
                     /gene="tolR"
                     /locus_tag="JQH65_002984"
     CDS             6311..6739
                     /gene="tolR"
                     /locus_tag="JQH65_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HAY6140335.1"
                     /translation="MARARGRGRRELKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            6804..7985
                     /gene="tolA"
                     /locus_tag="JQH65_002985"
     CDS             6804..7985
                     /gene="tolA"
                     /locus_tag="JQH65_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HAY6140336.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEKAK
                     AEAEKKAAAEKAAADKKAAADKKAAEKAAAEKAAADKKAAAEKAAAEADDIFGELSSG
                     KNAPKTGGGAKGNNASPAGSGNSKNNGASGADINNYAGQIKSAIESKFYDASSYAGKT
                     CTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKPPSQAVYEVFKNAPLDFK
                     P"
     gene            8118..9410
                     /gene="tolB"
                     /locus_tag="JQH65_002986"
     CDS             8118..9410
                     /gene="tolB"
                     /locus_tag="JQH65_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HAY6140337.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPSAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            9445..9966
                     /gene="pal"
                     /locus_tag="JQH65_002987"
     CDS             9445..9966
                     /gene="pal"
                     /locus_tag="JQH65_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HAY6140338.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            9976..10767
                     /gene="cpoB"
                     /locus_tag="JQH65_002988"
     CDS             9976..10767
                     /gene="cpoB"
                     /locus_tag="JQH65_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HAY6140339.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            10932..11007
                     /locus_tag="JQH65_002989"
     tRNA            10932..11007
                     /locus_tag="JQH65_002989"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10965..10967,aa:Lys,seq:ttt)
     gene            11043..11118
                     /locus_tag="JQH65_002990"
     tRNA            11043..11118
                     /locus_tag="JQH65_002990"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11076..11078,aa:Val,seq:tac)
     gene            11121..11196
                     /locus_tag="JQH65_002991"
     tRNA            11121..11196
                     /locus_tag="JQH65_002991"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11154..11156,aa:Lys,seq:ttt)
     gene            11248..11320
                     /locus_tag="JQH65_002992"
     tRNA            11248..11320
                     /locus_tag="JQH65_002992"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11281..11283,aa:Val,seq:tac)
BASE COUNT         2798 a         2655 c         3026 g         2842 t
ORIGIN      
        1 aattgaagaa agctctgatg atggtgatga atatgactat tcacccgcaa aagaagagtg
       61 ggtaattacc gcagcgaacg cgaaaccgca atgcgatatt attcatgaag cctggcagag
      121 cagggctgtt atccgctatg acatggcagt gccgctcaat ttgtcagaac gcagcgcccg
      181 gcaatccact ggcagagtag gggtggtgtt ggttgtcact cttagtcata acagcaggcg
      241 tattgatgtg gatatcaacc ttgataacca ggctgacgat catcgccttc gtgtcctggt
      301 ccctacacct tttaacaccg acagtgttct ggcagatacg cagtttggtt cgctaacgcg
      361 ccccgtgaac gacagtgcaa tgaacaactg gcagcaagaa ggctggaaag aagcgccggt
      421 tccggtatgg aatatgctca actatgttgc cttacaggaa gggcgtaacg gcatggctgt
      481 ctttagcgaa gggttacgtg aatttgaagt catcggtgaa gagaagaaaa cctttgccat
      541 tacgttgctg cgtggcgtgg gcttactggg caaagaagat ctgcttttaa ggcctgggcg
      601 gccttcggga attaaaatgc cagtcccgga ctcacaacta cgtggtctgc tttcttgtcg
      661 cctaagttta ttgagttata ccggtacgcc aaccgccgct ggtgtagctc agcaggcgcg
      721 agcatggctg actccagtac agtgttacaa caaaatccca tgggatgcga tgaagctcaa
      781 caaagccgga ttcaacgtgc cggaaagtta tagtttgttg aaaatgcccc cagtgggatg
      841 cctgataagc gcacttaaga aagctgaaga ccgacaagaa gtgattttac ggctgtttaa
      901 tccggctgaa tcagcaacct gtgatgcgac tgttgctttc agtcgcgagg tgatttcttg
      961 ctcagaaacg atgatggatg aacacattac caccgaggaa aatcaaggtt caaatctatc
     1021 ggggcctttt ttacccggcc agtcacggac gttcagttac cggcttgcct gaatagcaat
     1081 caaaccgaag ccacatatgc gcggccagat tgttgacaaa gggcgctttg ttcatgccgg
     1141 atacggcatg aacgctttat tcggtctaca aaagcaggca aattcaatat attgcagaga
     1201 tgatgtagac actggcaagc gtagcgcatc aggtaatttt gcgtttatct tcactctcaa
     1261 gccacgtata tgtggcttta tttttaacaa aataataacc ctgggtgagt taattataat
     1321 ataattataa gttaattaaa tgttaatatc ggcgggggtg gatttatgcc tttattagta
     1381 atcctgaaac tctgcgtcgt attagccagt gaccaaaaaa agaattaagg tcaaccgtgc
     1441 tgtttttgct tcgtctcttt ttatctttaa ttgccaaccg aaactaattt cagccttata
     1501 actcacacat tttaaacata aatgtcacta aagttacctt attgaaacat gattaacata
     1561 atttgtagga attgatattt atcaatgtat aagtcttgga aatgggcatc aaaaagagat
     1621 aaattgttct cgatcaaatt ggctgaaagg cggtaattta gctataaatt gatcaccgtc
     1681 gaaaaatgca aatttgcttc aacaaaaacc tgtttattgt aaggattttg cggcgtaata
     1741 tatacgtggg atcaatttga gtttttatta acatgtttgc aacctttctt tacgccgttt
     1801 ttgtgtgcat tcacatggta tgatgaaagt gttcaaacaa atttctattg gggcatgcgt
     1861 gtgacccttt ctaacggggt tcactctcgg agtcttcatg cgatgagcaa ggagtcatga
     1921 tgttagatat agtcgaactg tcgcgcttac agtttgcctt gaccgcgatg taccacttcc
     1981 tttttgtgcc actgacgctc ggtatggcgt tcctgctggc cattatggaa acggtctacg
     2041 tcctctccgg caaacagatt tataaagata tgaccaagtt ctggggcaag ttgtttggta
     2101 tcaacttcgc tctgggtgtg gctaccggtc tgaccatgga gttccagttc gggactaact
     2161 ggtcttacta ttcccactat gtaggggata tcttcggtgc gccgctggca atcgaaggtc
     2221 tgatggcctt cttcctcgaa tccacctttg taggtctgtt cttcttcggt tgggatcgtc
     2281 tgggtaaagt tcagcatatg tgtgtcacct ggctggtggc gctcggttca aacctgtccg
     2341 cactgtggat tctggttgcg aacggctgga tgcaaaaccc aatcgcgtcc gatttcaact
     2401 ttgaaactat gcgtatggag atggtgagct tctccgagct ggtgcttaac ccggttgctc
     2461 aggtgaaatt cgttcacact gtagcgtctg gttatgtgac tggcgcgatg ttcatcctcg
     2521 gtatcagcgc atggtatatg ctgaaaggtc gtgacttcgc cttcgctaaa cgctcctttg
     2581 ctatcgctgc cagcttcggt atggctgctg ttctgtctgt tattgttctg ggtgatgaat
     2641 ccggctacga aatgggcgac gtgcagaaaa ccaaactggc tgctattgaa gccgagtggg
     2701 aaacgcaacc tgcgcctgct gcctttactc tgttcggcat tcctgatcag gaagaggaga
     2761 cgaacaaatt tgcgattcag atcccttacg cactgggcat cattgcaacg cgttccgtgg
     2821 ataccccggt tatcggcctg aaagagctga tggtgcagca tgaagaacgc attcgtaacg
     2881 ggatgaaggc gtactctctg ctcgaacaac tgcgttctgg ttctaccgac caggcggttc
     2941 gtgaccagtt caatagcatg aagaaagacc tcggttacgg tctgctgctg aaacgctata
     3001 cgccaaacgt ggctgatgcg actgaagcgc agattcaaca ggcaactaaa gactccattc
     3061 cgcgtgtagc gccgctgtac ttcgcgttcc gtattatggt ggcgtgtggc ttcctgctgc
     3121 tggcaatcat cgcgctctct ttctggagtg tcatccgcaa ccgcattggc gagaaaaaat
     3181 ggctgctgcg cgccgcgctg tacggtattc cgctgccgtg gattgctgta gaagcgggct
     3241 ggttcgtggc tgaatatggc cgccaaccgt gggctatcgg tgaagtgctg ccgacagctg
     3301 tggcgaattc gtcactgacc gcaggcgatc tcatcttctc aatggtgctg atttgcggcc
     3361 tgtataccct gttcctggtg gcagaattgt tcttaatgtt caaatttgca cgcctcggcc
     3421 caagcagcct gaaaaccggt cgctatcact ttgagcagtc ttccacgact actcagccgg
     3481 cacgctaaga caggagtcgt caaatgatcg attatgaagt attgcgtttt atctggtggc
     3541 tgctggttgg cgttctgctg attggttttg cagtcactga cggtttcgac atgggggtgg
     3601 gcatgctcac ccgtttcctc ggtcgtaacg acaccgagcg tcgaattatg attaactcca
     3661 ttgcaccaca ctgggacggt aaccaggttt ggctgatcac cgcgggcggc gcactctttg
     3721 ctgcctggcc gatggtctat gccgctgcgt tctccggctt ctatgtggcg atgatcctcg
     3781 tgctggcgtc tttgttcttc cgtccggtcg gttttgacta ccgctccaag attgaagaaa
     3841 cccgctggcg taacatgtgg gactggggca tcttcattgg tagcttcgtt ccgccgctgg
     3901 taattggtgt tgcgttcggt aacctgttgc agggcgtacc gttcaacgtt gatgaatatc
     3961 tgcgtctgta ctacaccggt aacttcttcc agttgcttaa cccgttcggc ctgctggcag
     4021 gcgtggtgag cgtagggatg atcattactc agggcgcaac ctatctgcaa atgcgtaccg
     4081 tgggcgaact gcacctgcgt acccgtgcaa cggctcaggt ggctgcgctg gtgacactgg
     4141 tctgtttcgc actggctggc gtatgggtga tgtacggtat cgatggttat gtcgtgaaat
     4201 cgacaatgga ccattacgca gcctctaacc cactgaataa agaagtggtt cgtgaagctg
     4261 gcgcatggct ggttaacttc aacaacacgc caattctgtg ggctattccg gcactgggtg
     4321 tggttctgcc gctgctgacc atcctgactg cacgtatgga taaagccgcg tgggcgtttg
     4381 tgttctcctc cctgacgctg gcctgcatca tcctgacagc cggtatcgca atgttcccgt
     4441 ttgtgatgcc gtccagcacc atgatgaacg caagtctgac aatgtgggat gcaacttcca
     4501 gccagctgac gcttaacgtc atgacctggg ttgcggtggt tctggtaccg atcattctgc
     4561 tctacaccgc ctggtgttac tggaaaatgt tcggtcgtat caccaaagaa gatattgaac
     4621 gtaacaccca ctctctgtac taagtaagga gctaaaaatg tggtatttcg catggattct
     4681 gggaacgctt cttgcctgtt cgtttggggt aatcaccgcg ctggcgcttg aacacgtcga
     4741 atcaggcaaa gccggtcaag aagacatctg atgagtaaga ttatcgcgac cttgtatgcg
     4801 gtaatggaca agcgccccct gcgggcgctt tccttcgtga tggcgcttct gttagcagga
     4861 tgtatgtttt gggacccatc tcgttttgcc gcgaagacca gtgaactgga aatctggcat
     4921 ggtttattgc tgatgtgggc cgtctgtgct ggtgtgattc acggcgtggg ctttcgtccg
     4981 cagaaggttc tttggcaagg gattttttgc ccattgcttg ccgatattgt tctcattatc
     5041 gggctgattt tcttcttctt ttaaatcaga attctcttta aaaaattatg ggccgctcca
     5101 ggcccataaa tttttacgct cccttaactt gccctcattc ccaaacctca atcgcgcgcg
     5161 tatagtagca gcgtttaaaa gctctaactt ttgttgcatt accgggatgt aaagtgaata
     5221 caacgctgtt tcgatggccg gctcgcgtct actatgaaga taccgatgcc ggtggtgtgg
     5281 tgtaccacgc cagttacgtc gctttttatg aaagagcacg cacagagatg ctgcgtcatc
     5341 atcacttcag tcagcaggcg ctgatggctg aacgcgttgc ctttgtggta cgtaaaatga
     5401 cggtggaata ttacgcacct gcgcggctcg acgatatgct cgaaatacag actgaaataa
     5461 catcaatgcg tggcacctct ttggttttca cgcaacgtat tgtcaacgcc gagaatactt
     5521 tgctgaatga agcagaggtt ctggttgttt gcgttgaccc actcaaaatg aagcctcgtg
     5581 cgcttcccaa gtctattgtc gcggagttta agcagtgact gacatgaata tccttgattt
     5641 gttcctgaag gctagccttc tggttaaact tatcatgttg attttgattg gtttttcaat
     5701 cgcatcctgg gccattatta tccagcggac ccgtattctt aacgcagcgg cgcgcgaagc
     5761 cgaagcgttt gaagataaat tctggtctgg aatcgaactc tctcgcctct atcaagagag
     5821 tcaggggaaa cgggataatc tgactggttc ggaacaaata ttttacagcg ggttcaaaga
     5881 gtttgtgcgc ctgcatcgtg ccaatagcca tgcgccggaa gccgtagtgg aaggggcgtc
     5941 gcgtgctatg cgtatctcca tgaaccgtga acttgaaaat ctggaaacgc acattccgtt
     6001 cctcggtacg gttggctcca tcagcccgta tattggtctg tttggtacgg tctgggggat
     6061 catgcatgcc tttatcgccc tcggggcggt aaaacaagca acactgcaaa tggttgcgcc
     6121 cggtatcgca gaagcgttga ttgcgactgc aattggtctg tttgccgcta tcccggcagt
     6181 tatggcctac aaccgcctca accagcgcgt aaacaaactg gaactgaatt acgacaactt
     6241 tatggaagag tttaccgcga ttctgcaccg ccaggcgttt accgttagcg agagcaataa
     6301 ggggtaagtc atggccagag cgcgtggacg aggtcgtcgc gaacttaagt ccgaaatcaa
     6361 cattgtaccg ttgctggacg tactgctggt gctgttgctg atcttcatgg cgacagcgcc
     6421 catcatcacc cagagcgtgg aggtcgatct gccagacgct actgaatcac aggcggtgag
     6481 cagtaacgat aatccgccag tgattgttga agtgtctggt attggtcagt acaccgtggt
     6541 ggttgagaaa gatcgcctgg agcgtttacc accagagcag gtggtggcgg aagtgtccag
     6601 ccgcttcaag gccaacccga aaacggtctt tctgatcggt ggcgcaaaag atgtgcctta
     6661 cgatgaaata attaaagcac tgaacttgtt acatagtgcg ggtgtgaaat cggttggttt
     6721 aatgacgcag cctatctaaa catctgcgtt tcccttgctt gaaagagagc gggtaacagg
     6781 cgaacagttt ttggaaaccg agagtgtcaa aggcaaccga acaaaacgac aagctcaagc
     6841 gggcgataat tatttcagca gtgctgcatg tcatcttatt tgcggcgctg atctggagtt
     6901 cgttcgatga gaatatagaa gcttcagccg gaggcggcgg tggttcgtcc atcgacgctg
     6961 tcatggttga ttcaggtgcg gtagttgagc agtacaaacg catgcaaagc caggaatcaa
     7021 gcgcgaagcg ttctgatgaa cagcgcaaga tgaaggaaca gcaggctgct gaagaactgc
     7081 gtgagaaaca agcggctgaa caggaacgcc tgaagcaact tgagaaagag cggttagcgg
     7141 ctcaggagca gaaaaagcag gctgaagaag ccgcaaaaca ggccgagtta aagcagaagc
     7201 aagctgaaga ggcggcagcg aaagcggcgg cagatgctaa agcgaaggcc gaagcagatg
     7261 ctaaagctgc ggaagaagca gcgaagaaag cggctgcaga cgcaaagaaa aaagcagaag
     7321 cagaagccgc caaagccgca gcgcagaaaa aagccgaggc agccgctgcg gcactgaaga
     7381 agaaagcgga agcggcagaa gcagctgcag ctgaagcaag aaagaaagcg gcaactgaag
     7441 ctgctgaaaa agccaaagca gaagctgaga agaaagcggc tgctgaaaag gctgcagctg
     7501 ataagaaagc tgcagccgac aaaaaagcag cagaaaaagc ggctgctgaa aaggcagcag
     7561 ctgataagaa agcagcggca gaaaaagccg ccgcagaggc agatgatatt ttcggtgagc
     7621 taagctctgg taagaatgca ccgaaaacgg ggggaggggc gaaagggaac aatgcttcgc
     7681 ctgccgggag tggtaattct aaaaacaatg gcgcatcagg ggccgatatc aataactatg
     7741 ccgggcagat taaatctgct atcgaaagta agttctatga cgcatcgtcc tatgcaggca
     7801 aaacctgtac gctgcgcata aaactggcac ccgatggtat gttactggat atcaaacctg
     7861 aaggtggcga tcccgcactt tgtcaggctg cgttggcagc agctaaactt gcgaagatcc
     7921 cgaaaccacc aagccaggca gtatatgaag tgttcaaaaa cgcgccattg gacttcaaac
     7981 cgtaatcgcg atgttgactg ttcggacggt caacatcagg caccggttgc cacggggttc
     8041 tggtagtttt gtgtatttta gtttgttaac attctgctaa attatcgtgg gccatcggtc
     8101 cagataaggg agatatgatg aagcaggcat tacgagtagc atttggtttt ctcatactgt
     8161 gggcatcagt tctgcatgct gaagtccgca ttgtgatcga cagcggtgta gattccggtc
     8221 gtcctattgg tgttgttcct ttccagtggg cggggcctgg tgcggcacct gaagatattg
     8281 gcggcatcgt tgctgctgac ttgcgtaaca gcggtaaatt taatccgtta gatcgcgctc
     8341 gtctgccaca gcagccgggt agtgcgcagg aagtacaacc atctgcatgg tccgcactgg
     8401 gcattgacgc tgtagttgtc ggtcaggtca ctccgaatcc ggatggttct tacaatgttg
     8461 cttatcaact tgttgacact ggcggcgcac cgggtactgt acttgctcag aactcgtaca
     8521 aagtgaacaa gcagtggctg cgttatgctg gtcataccgc cagtgatgaa gtgtttgaaa
     8581 aactgaccgg cattaaaggt gcgttccgta cccgtattgc ctacgttgtt cagaccaacg
     8641 gcggtcagtt cccgtatgaa ctgcgcgtat ctgactatga cggttacaac cagtttgtcg
     8701 ttcaccgttc accgcagccg ctgatgtctc cggcgtggtc accagacggt tctaaactgg
     8761 cttatgtgac cttcgaaagc ggtcgttccg cgctggttat tcagacgctg gcaaatggcg
     8821 ctgtacgtca ggtggcttca ttcccgcgtc acaacggtgc acctgcattc tcgccagacg
     8881 gcagcaaact ggcattcgcc ttgtcgaaaa ccggtagtct gaacctgtac gtaatggatt
     8941 tggcttctgg tcagatccgc caggtgactg atggtcgcag taacaatacc gaaccgacct
     9001 ggttcccgga tagccagaac ctggcattta cttctgacca ggccggtcgt ccgcaggttt
     9061 ataaagtgaa tatcaacggc ggtgcgccac aacgtattac ctgggaaggt tcgcagaacc
     9121 aggatgcgga tgtcagcagc gacggtaaat ttatggtaat ggtcagctcc aatggtgggc
     9181 agcagcacat tgccaaacaa gatctggcaa cgggaggcgt acaagttctg tcgtccacgt
     9241 tcctggatga aacgccaagt ctggcaccta atggcactat ggtaatctac agctcttctc
     9301 aggggatggg atccgtgctg aatttggttt ctacagatgg gcgtttcaaa gcgcgtcttc
     9361 cggcaactga tggacaggtc aaattccctg cctggtcgcc gtatctgtga taataattaa
     9421 ttgaatagta aaggaatcat tgaaatgcaa ctgaacaaag tgctgaaagg gctgatgatt
     9481 gctctgcctg ttatggcaat tgcggcatgt tcttccaaca agaacgccag caatgacggc
     9541 agcgaaggca tgctgggtgc cggcactggt atggatgcga acggcggcaa cggcaacatg
     9601 tcttccgaag agcaggctcg tctgcaaatg caacagctgc agcagaacaa catcgtttac
     9661 ttcgatctgg acaagtacga tatccgttct gacttcgctc aaatgctgga tgcacatgca
     9721 aacttcctgc gtagcaaccc gtcttacaaa gtcaccgtag aaggtcacgc ggacgaacgt
     9781 ggtactccgg aatacaacat ctccctgggt gaacgtcgtg cgaacgccgt taagatgtac
     9841 ctgcagggta aaggcgtttc tgcagaccag atctccatcg tttcttacgg taaagaaaaa
     9901 cctgcagtac tgggtcatga cgaagcggca tactccaaaa accgtcgtgc ggtactggtt
     9961 tactaagaga attgcatgag cagtaacttc agacatcaac tattgagtct gtcgttactg
    10021 gttggtatag cggccccctg ggccgctttt gctcaggcac caatcagtag tgtcggctca
    10081 ggctcggtcg aagaccgcgt cactcaactt gagcgtattt ctaatgctca cagccagctt
    10141 ttaacccaac tccagcaaca actttctgat aatcaatccg atattgattc cctgcgtggt
    10201 cagattcagg aaaatcagta tcaactgaat caggtcgtgg agcggcaaaa gcagatcctg
    10261 ttgcagatcg acagcctcag cagcggtggt gcagcggcgc aatcaaccag cagcgatcaa
    10321 agcggtgcgg cggcatcaac gacgccgaca gctgatgctg gtactgcgaa tgctggcgcg
    10381 ccggtgaaaa gcggcgatgc aaacacggat tacaatgcag ctattgcgct ggtgcaggat
    10441 aaatcccgcc aggatgacgc aatggtggca tttcagaatt tcatcaaaaa ttaccctgat
    10501 tcaacttacc tgccaaacgc caattattgg ctgggtcagt taaactacaa caagggtaaa
    10561 aaagatgatg cggcgtacta ttttgcttcg gtagtgaaaa actatccgaa gtcaccaaag
    10621 gctgctgatg cgatgtttaa agtcggcgtc atcatgcagg acaaaggtga caccgcaaaa
    10681 gcgaaagccg tataccagca ggttatcagt aaataccctg gtactgatgg cgctaaacag
    10741 gcacaaaaac gtctgaacgc gatgtaatgc ataacacacg accagaagtt gcattatttc
    10801 tggtcgtgtc gtgcgaatca taagcagttg agtgatctgc atcgaaattt ttgttgcgct
    10861 caagtctgaa atcagtaata tatgccgccg ttgccacggg atatcaaaca aaccgaaagc
    10921 aacgaaaaag tgggtcgtta gctcagttgg tagagcagtt gacttttaat caattggtcg
    10981 caggttcgaa tcctgcacga cccaccacta acatagttag ttgtagtatc cagcgtagta
    11041 ttgggtgatt ggctcagctg ggagagcacc tcccttacaa ggagggggtc ggcggttcga
    11101 tcccgtcatc acccaccacc gggtcgttag ctcagtcggt agagcagttg acttttaatc
    11161 aattggtcgc aggttcgaat cctgcacgac ccaccactaa ttacgatggg ttcggtggaa
    11221 gtagtttgta gtatccagcg cattatcggg tgattagctc agctgggaga gcacctccct
    11281 tacaaggagg gggtcggcgg ttcgatcccg tcatcaccca c
//
LOCUS       DADLFA010000134        11212 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000134 DADLFA010000000
VERSION     DADLFA010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11212
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.134"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(1..868)
                     /locus_tag="JQH65_002993"
                     /pseudo
     CDS             complement(1..868)
                     /locus_tag="JQH65_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
     gene            complement(843..1643)
                     /gene="otsB"
                     /locus_tag="JQH65_002994"
                     /pseudo
     CDS             complement(843..1643)
                     /gene="otsB"
                     /locus_tag="JQH65_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
     gene            complement(1810..2799)
                     /locus_tag="JQH65_002995"
     CDS             complement(1810..2799)
                     /locus_tag="JQH65_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose ABC transporter permease AraH"
                     /protein_id="HAY6140340.1"
                     /translation="MMSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVP
                     NFATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTES
                     LWIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIED
                     ESFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPV
                     VRTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGK
                     ISYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(2811..4325)
                     /locus_tag="JQH65_002996"
     CDS             complement(2811..4325)
                     /locus_tag="JQH65_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HAY6140341.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(4395..5383)
                     /locus_tag="JQH65_002997"
                     /pseudo
     CDS             complement(4395..5383)
                     /locus_tag="JQH65_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397210.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein"
     gene            6097..6600
                     /locus_tag="JQH65_002998"
     CDS             6097..6600
                     /locus_tag="JQH65_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HAY6140342.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(6679..6930)
                     /locus_tag="JQH65_002999"
     CDS             complement(6679..6930)
                     /locus_tag="JQH65_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HAY6140343.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFSNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(7045..7131)
                     /gene="azuC"
                     /locus_tag="JQH65_003000"
     CDS             complement(7045..7131)
                     /gene="azuC"
                     /locus_tag="JQH65_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HAY6140344.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            7394..7717
                     /locus_tag="JQH65_003001"
     CDS             7394..7717
                     /locus_tag="JQH65_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6140345.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            7888..8385
                     /gene="ftnA"
                     /locus_tag="JQH65_003002"
     CDS             7888..8385
                     /gene="ftnA"
                     /locus_tag="JQH65_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HAY6140346.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQHLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(8423..8662)
                     /locus_tag="JQH65_003003"
     CDS             complement(8423..8662)
                     /locus_tag="JQH65_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HAY6140347.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            8853..10064
                     /gene="tyrP"
                     /locus_tag="JQH65_003004"
     CDS             8853..10064
                     /gene="tyrP"
                     /locus_tag="JQH65_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HAY6140348.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRRVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVR"
     gene            complement(10126..10791)
                     /gene="yecA"
                     /locus_tag="JQH65_003005"
     CDS             complement(10126..10791)
                     /gene="yecA"
                     /locus_tag="JQH65_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HAY6140349.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTNHAILDVAELDDLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(10987..11073)
                     /locus_tag="JQH65_003006"
     tRNA            complement(10987..11073)
                     /locus_tag="JQH65_003006"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11037..11039),aa:Leu,seq:taa)
     gene            complement(11086..11159)
                     /locus_tag="JQH65_003007"
     tRNA            complement(11086..11159)
                     /locus_tag="JQH65_003007"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11125..11127),aa:Cys,seq:gca)
BASE COUNT         2804 a         2847 c         2595 g         2966 t
ORIGIN      
        1 taacgaattt taccatgatg ctgcggatat ttttccagca acgcttcata ggcgagaaaa
       61 cgctctggca aacctttgga ataatccagc cgttcgacag aaaagatatt ttgtacgttt
      121 ttcagttccg ctttaagttg cgccagtttt ggcggcagtg gcccggcagc ctgtttggct
      181 atttctttcg gttcaatgcc gatcgggtag acttctgttc gaaatgcttt gccccaggct
      241 gtatggcttt ttgcgctacg tgtcgtgacg cgggtcaggt tagaaagaca atccaggaac
      301 gccagacgat cgttttctgt ctggaaaccc agcaaatcat aatcacaaag ctgttcaagc
      361 aaggtgtcat atgtcggcag cgcgttgaag atttccggtg tcgggaaagg aatatgcaga
      421 aagaaaccaa tgcgattatt caccccacgt ttgcgtaatt catgcgcaaa gggcaacagg
      481 tgataatcgt ggatccagat aatgtcatcg tcttgcaaca gcggcagtaa tttatctgcc
      541 agcaacgcat ttacgcgtag atagccgtcc caggcaggac gctgaaattg catcagatcg
      601 agccgataat gaaaagcggg ccagagaacg gcattggaga attggttgta gtattcgtca
      661 aggtcctgtt cgctgaggtt aaaagaggcc cacgtaatgt tacctttttt agcggctgat
      721 cctcattccc tgtttcacca ctccagccaa accacagtcc gcctgtggct ttcagtgccc
      781 ccagtatgcc aacggcaagg ccaccggcac tggcggcgtg ctcgtctggt ggtgcaatcc
      841 ggttagatac tacgactaaa cgactcatag tcatcactcc tgttattttc tcttttttgt
      901 tgtaatgcgg tggttatcat ttcaagcctg ctccagacat ccggcacacc cgccagtcgc
      961 catgatgcct gagttgcacc tgtgccaatt tttactgaca ttccgcccag tcggttaacg
     1021 actgcgaagc cagattcatc ggttaaatca tcgcccagaa atacgggcgt tcgcccgata
     1081 aagggagctt cctgcataaa agctgcaatt gcctcacctt tactggtacc tctcggtttg
     1141 atctcgacaa cacactttcc ctgctgtaac gccatttgtg gccagatctg agtaatacgt
     1201 tacgctaatg tcattaatgc gtcttcatgc tgcggagcct gacgataatg cagcgcaaaa
     1261 gccatccctt tcgcctccag ctccgcgccg ggatactgag cgataactgt atgcagttgc
     1321 acgctaatat cacgcgcaat cgcatccggc agatgaacga tatgcgtttt accattgatg
     1381 tcacggcgct ccgccccatg cacgcccgct aacgggaagc gataaggttt tgccagtgcg
     1441 tcaagctcca ccattgagcg ccctgatatc aatgccaatg caccatcact tgcggttgcc
     1501 agtagctgta gtccttgcag aatattgtca ggcacgacga cctgatcggg atgcggtttg
     1561 atttccgcca gcgttccatc gagatcaaaa aaccaggcat atttcgcgga tagttcaggg
     1621 gtttcggtta acggttctgt cacccggttc tcctacttct cttttcttat tgttttgcgc
     1681 agaacgcgct ttacagacat gtaagtatag acagtgtgac gggggtcgcc atttgcaacg
     1741 aagaacagcc aaccgttatg gcggttggct gtggtgggaa aaaacgctaa attgttgcag
     1801 aaaaaagcat cagacagtgc gtttcgcttt ttgcttgtaa cggtcgaaga tcaccgctgc
     1861 cagcaggatt aagccgcgaa ccacgtactg cgcgaaagga gaaatattaa gcaggttcat
     1921 ggcgttttcc acggtgccta aaattaagat acccgccacc acatatgaga tttttccgat
     1981 gcctcctttc agagaaacgc cacctaaaac gcaggcggag ataacaatca gctcgtaacc
     2041 aatcgacgtc attggctgcc cactggtcat acgtgaagcc agaataattc cggctatcgc
     2101 agataccagg ccagagagaa caaagataat aattttggtg cgaacaaccg gtacacccgc
     2161 cagacgcgca gcctcttcgt tcccgccaat cgccagggtg ttacgaccaa aggtggtttt
     2221 attcagcaac aaaccaaaga taatcagaca cgcgacggtg agccagattg gcgcaggcag
     2281 accgaaccag ttagcgtaac caagggcaaa gaagctttca tcttcgatac cgaccgcttt
     2341 accgtctgaa atgatgtacg ccagacctcg aacaatctgc atcgttgcca gtgttgtgat
     2401 cagagcattt attttcagtt tggcgataac aaagccattg accaggccac agagaatgcc
     2461 aagcagcaac cccgctgcca cgccaatcca caggctttca gtcaggttga taaccaccgc
     2521 cgtggtgaca cccgcacagg caattacgga ggcgacagaa aggtcaaagt caccggaagc
     2581 aaggcagaac aacatgccac acgccaccat ccccgacatg gaaattgcca ggcccaaccc
     2641 tttcatatta atgaaggtgg caaaatttgg gacaaaaatg gcacaggcga taaagagcac
     2701 cgcaaacacc accagcatgc cgtactgatc ccagatacgc ccgaagctga atgacgactt
     2761 aggtgcgcca gaacccgatg tagaaacaga agacatcata ctctccttac tcaggcaact
     2821 gcctggctga ctttaggcat cgcaaggctc agtgcctgac gctcatctgc ctgctcgtgt
     2881 aacaattcac cggcgatttc accttcccgc atcaccacaa tccggtcggc aacgccgagg
     2941 acttcaggta agtcgctgga ggcaaacagc accgccacac cctgcgccgc cagcgcataa
     3001 attacgttat atatttcgtg cttagcacca acatcaatgc cgcgcgtagg ttcatccagc
     3061 aaaatgacct tcatctcttc cgataaccag cggcccagaa tggctttttg ctgatttccg
     3121 cctgagagat tcatgatcag ttgctcagcg cccggcgttt tgatgttgag cgaacgaatg
     3181 tgctgatcgg cattgttttc ttcccaaccg ttgttgatta cacaaccgcc gagcacatgt
     3241 ttgcgtctgg cactgatgtt gatattgtcg cgaacggagt gcacgggaat aatgccttcc
     3301 gctttgcgat cttccgggca gagcatcatg cctgcggcaa tggcgtggct cggtttacga
     3361 atatcgatcg gctgttggtc gatataaacc tgaccggcgg tgatttgcgt cccgccaaac
     3421 aagcctttca ttaattcgct acgccccgcc cctaccagac caaacagccc aacaatttca
     3481 ccactgcgaa ccgccagact tattggcgta cgcacgcctg gtgctttcac agcatcaaga
     3541 cgcaggcgct cctcgccata actacgaggt tgccagccgt agatatcgcc aatgtcgcgc
     3601 ccgaccatcg cctgcaccag cgcgtcgtgg tcaacctgct gcatatcggt aaaggttttg
     3661 acataacgtc catctttaaa gacagtgatg gcatcgctga gggcaaatat ttcttccata
     3721 cggtgagaaa cgtataagat cacccgcccc tcttttcgca gttcacgaat aacgcggaaa
     3781 agattgtcga tttcgcgggc agaaagggag ctggttggct catcaaaggc gataatttta
     3841 gcgttacgcg ccagcgcctt ggcgatttca accatctgcc actgaccaat ggagagatat
     3901 ttcagcggcg tgtccgggtc aatatccata ccaagatgtt taagttgtaa acccgcctca
     3961 taattcagca atgagcgatt cacaatgccg cctttatgcg gcagctggcc gagatagatg
     4021 ttttccgcga cggtcatttc cggcacgaga tgcagttcct ggtaaataat cgccacaccc
     4081 gcgttaagtg ctgcggtcgt gtcggaaaag gacatttcct gcccattaat cactacagaa
     4141 cccgtggttg gcgcatagtt gccgctgagg atttttaaga gagttgattt tcctgcgcca
     4201 ttttcaccca tcaacgcatg aacctgaccg gcatagcagt caaaactaat atccgtcagc
     4261 gccttaacgc cgggaaacgt tttaccgatg ccgcgaaatg agagatacgg ggtagactgt
     4321 tgcataacgt ctccgtgaat cactggtcgt tcacaccccc tctgcatcat gcagagggga
     4381 atttttccgg caaattactt accgcctaaa ccttttttct ccagttcttc tttaaagttg
     4441 tcacgcgtga tcagcaccac gtcggtaact tcggtaaatt ttggcggttc aacgtctttt
     4501 gctacccagt tgtaaagcat ttcgctggat ttatagccat gtacgtccgg gcttggcagc
     4561 agggaaccgt agaagccggt tgcctgtact ttagacagtt cgctcaccgc atccacaccg
     4621 ttaatgccaa tgccgataat atcggcagct ttaaagccct gaccttccgt cgcgcgtacg
     4681 ccgcccagca cggtgctgtc gttcataccg acgatcagcc aatgtttaac ttcaggatgt
     4741 tgaaccagca ttgagttggc agcgtcaaat gcccccggga tgtcgttaga tttggtaggt
     4801 acctgataaa tttgtttttc cgggaatccg gccgctttca gcgcatccat agatcccgta
     4861 gtacggcggc gggcagtatc cagttcgttg gcggtaatca ccatcaccgc gctttctttg
     4921 acatcccagc cacgtttctg catctcttta tacagttcct gaccctgacg ttcgccaatt
     4981 ttagtcgcgc catcatcacc agcggaacgg tatccattgg cttacctttg gcgttaacaa
     5041 actgatcatc cacggcaatg actttcatat cgtagccacg cgctttcgcg acgatggcag
     5101 agccgagttt ggggtccgga gtacaaataa cgaaaccttt tgcgccactg gcagccaggc
     5161 tgtcgatcgc gttcaatgtt ttttcgccat ccggcacggc aatcttaata acctcaaacc
     5221 ctaaatcctt cccggcttta tcggcaaact tccattcggt ctggaaccac ggctcttccg
     5281 gttgcttcac cagaaaaccg agcttcaggt tctccgccat agcggattgt gacataacgg
     5341 ctgccagacc aatggctgcc agggctttag taaatttgtg catggttctc tccagcttta
     5401 gtgtcgtttt gtgtagggca aaaacgaatg acattcgtta aattaatcgg aaaacaaagc
     5461 attacctttt aactaaaaga taagtgaccg tgttgacata gttttagcga gaaattaatt
     5521 ctccatagga gagcaatatc acatcgcaga attacagtga gaacgtgcat aaatttagcg
     5581 ggaaaagaca taagggaaag ccaatttgtc agacaaattg tcgaatgcac agcagattaa
     5641 tccataagat tagcttggaa atccttgttg tctttggtac ccatgcggga tgtcttcttt
     5701 ttaaccagtc aataggccgc attacctggc gttgagtttt tgaaatggtg taataaccgc
     5761 aactcaaaga tgtggaaaat gcacgtcatt catttcgcca ttaattatca ctgtgctcat
     5821 taattaacag aacacgtata atgagagcca tctcgcaaaa atgaaaaaac gttttataaa
     5881 atcatcattt catcaatgaa atgtaaaaat atataaactt gatgatttaa gcattttttt
     5941 atacccgttc agacgttatt cttatttcag atcatcgtca gaattgactc cacgatcaca
     6001 tttcggaccg gcagaaagga attattctgc aaacagtaat tatggtgttt tgatttatct
     6061 tgcacctctc cacttctgga tataaggata ttaggtatgg caaccgctgg aatgcttctc
     6121 aaactcaact ctcaaatgaa ccgcgagttt tacgcatcca atctctacct tcacctgagt
     6181 aactggtgtt ctgaacagag tctgaacggc accgccactt tccttcgcgc ccaggcacag
     6241 agtaatgtga cccaaatgat gcgcatgttt aactttatga agagtgtcgg cgctaccccc
     6301 atcgttaaag ccattgatgt tcccggtgaa aaactgaact ctctggaaga actgttccaa
     6361 aaaacgatgg aagaatacga gcaacgttct agtacgttgg cacagttagc cgatgaagcg
     6421 aaagaactga atgatgattc aaccgtcaat ttcctgcgcg atctggaaaa agaacagcag
     6481 catgatggtc tgttgctgca aaccattctt gatgaagtgc gcagtgcgaa acttgctggt
     6541 atgtgcccag tgcagaccga ccaacatgtt ctgaatgtcg tgtcgcatca gctgcattga
     6601 tcatacatcg gcgctaatgc attgcgccga tgaagttttt gagaaaccgc tgcctcatct
     6661 ttttgaagca gcggtttttt aatgggattc gccttgtggg gtaaacttga gttcaatcag
     6721 cgcgatggct ttttggattg cccgcatggt gaccgggtct gcggcggcgg gatggttact
     6781 aaagtcgata ttcttcagct gactggacat tttttcacga acttcaacgg gcgcgattac
     6841 atcgagaaca tccagaattt gtttgataac caactggcaa gcaaccacat cagaaaccag
     6901 ttcctgatcg gcattcagcg gctgggacat cgtaaactcc tgatagcatt ttgaaagccg
     6961 ttatagtagc ggcttcacat cttcagcgat agtcacatcc accgtcatca ggacacaaaa
     7021 aaacctgccg gagcaggttt tttgttatcg gaacatattg cctggcggta cgtctttgaa
     7081 cgtcttgcaa tagttattga acatactttt caggattttg cgcagtttca tcgcggcact
     7141 ccgaccattt gttatacatg tgttattttc tttgtgctta taatatgaca accatcacaa
     7201 aaatcaatct ttatgtgatg caaatcacac aaataccccc ttaatgttat aaaaatgata
     7261 atcaaaaaac agccccccca tttctgacac ctacagatgg caagaaatag cgcctgccag
     7321 gcgtcttttc cggtcattgt cgcagcgctg taacgcgtaa aatagtgctt tcttttactc
     7381 ttctggctgg accatgagac ttctgattct gactctttca ttaataacgc tggcggggtg
     7441 tacggttact cgccaggccc atgtgagcga agttgacgcg gcaacaggta ttgtacggtt
     7501 ggtttatgac caggcttttt tgcagcatgc ccataccgat cgttatgtca gtcgcgggat
     7561 cgccgatcgc gcatgccagc aggaaggcta tacccacgcg atcccctttg gtcagccagt
     7621 aggcaactgt agcctttttg cgggttcctt atgtttgaat acggaattca ctttgtcata
     7681 tcagtgccat cattccgcct tccctgtctt tctctaaata ttttaatcag cgaggggatc
     7741 ttcgctgatt aaaaaaatag cggtaatgcg ttttattccc attcgtattt ttaataattg
     7801 aagtttatat tttacctttt gcaaataata aaataacaaa ttatagtgac gccacagcaa
     7861 caaatataac ctttgtggag cactatcatg ctgaaaccag aaatgattga aaaacttaat
     7921 gagcagatga acctggaact gtactcttca ctgctttatc agcaaatgag cgcctggtgc
     7981 agctatcata ccttcgaagg tgctgccgcg ttcctgcgcc gtcacgccca agaagagatg
     8041 acgcatatgc agcatctgtt tgattacctg actgataccg gcaatttacc gcgtattaat
     8101 accgttgaat ctccgtttgc tgaatattcc tcacttgatg aattattcca ggaaacctat
     8161 aaacacgaac aattaatcac ccagaaaatt aacgaactgg ctcatgctgc aatgaccaat
     8221 caggactacc caacatttaa tttcctgcaa tggtatgttt ctgagcagca tgaagaagag
     8281 aaactgttca aatcgattat tgataaatta agcctggcag gcaaaagcgg cgaaggtctg
     8341 tattttatcg acaaagaact ctctaccctc gacacacaaa actaatactc tccggcggca
     8401 ggtttactgc cgccgtttca aatcagtgac ggcaaatctt actctgatcg gtcgaaaaac
     8461 cgtctttcga aggtataaat ttgccttttg ctgccagaaa cgctaccagc tctcccgctg
     8521 tcatcccttc tgccgagcag gtgtgaaaac gtgcctgttc accaaaacgc gctttaattg
     8581 ctgcttccag actggcatgc gtatattgct cgcctgattc aatcatcata tttaacactt
     8641 catgaccgtg aatagagtcc atcgttcctc ctcaaaaaaa gcctagcgta gcgattgccg
     8701 cttatgaaga ctttgcgcca gagcagaact gaatgctttt tattgtacat ttatatttac
     8761 accatatgta acgtcggttt gacgaagcag ccgttatgcc ttaacctgcg ccgcagatat
     8821 cactcataaa gatcgtcagg acagaagaaa gcgtgaaaaa cagaactctg ggaagtgttt
     8881 ttatcgtggc gggaaccaca attggcgcag gcatgctggc aatgccgctg gctgcggcag
     8941 gtgttggttt tagcgtcacg ttaatcttgt tgattgggct ttgggcgttg atgtgctaca
     9001 cggcgctatt actgctggag gtgtaccagc atgttccggc agataccggt ctgggcacgc
     9061 tggcaaaacg ctatctggga cgctacggtc aatggctgac gggcttcagt atgatgttct
     9121 taatgtatgc tctgactgcg gcatacatca gcggtgccgg tgaattgttg gcctccagca
     9181 tcagcgactg gacaggtatt tcaatgtcgg caaccgctgg cgtgctgttg ttcacttttg
     9241 ttgccggtgg cgtggtttgt gttggaacat cgctggtcga tttatttaac cgttttctgt
     9301 tcagcgccaa aattattttt ctggtggtga tgctggtatt gctgctaccg catattcaca
     9361 aagtgaatct tttaaccctg ccgttgcaac aggggctggc tctgtcagca atcccggtga
     9421 tttttacctc atttggtttt cacggtagcg tgccgagtat tgtcagctat atggatggca
     9481 acgttcgtaa gctacgcagg gtgtttataa ccggtagtgc gatccccctg gtggcatata
     9541 ttttctggca ggtggcaacg cttggcagca ttgattcaac aacctttatg ggattgctgg
     9601 ctaatcatgc tggattaaac gggctgttac aggcgttacg cgaaatggtg gcctctccgc
     9661 atgttgagct ggcagtgcat ttatttgctg atttagccct cgccacgtca tttctcggcg
     9721 ttgcgttagg cttatttgat tatctggctg atttatttca gcgttcaaat accgttggtg
     9781 gacggttgca aactggtgca attacctttc tgccgccgtt ggcgtttgca ctgttttatc
     9841 cacgaggatt tgtgatggcg ctgggttacg ccggtgtggc gctggcggta ctggcattga
     9901 ttatcccttc gctgttgacc tggcaaagca gaaagcacaa tcctcaggcg ggttaccggg
     9961 tcaaaggtgg tcgtccggcg ctggtggtgg tgtttctctg tggtattgct gtgattggcg
    10021 tgcaattttt gattgcggca gggttgttac cagaagtgag gtgatcagat agcctcaaat
    10081 tccttattgg gtgccagaat taacgctgac acccaatttg gcctcttaat gcaggcagca
    10141 ctgcttaaat ttcttaccac taccgcacgg gcaaggatcg ttacgccccg gtttctcttc
    10201 tgctttgatc ggttgctgaa cagctttttc ctgcggatgc gccatccagt acgcatgtag
    10261 atcaagcgcc gccagtcgaa tggcatctac gctctcttca aacgcttctg gcgacatctt
    10321 ttctacccgc tcgaagtttt cctcagtacc gtgcagcgca atcgcctcca gcgctggttt
    10381 taacgaatcg ggcaacgttg accagtcaga aagtgccacg ccccgcatat agccaaagca
    10441 ccactcctca acaatcgtca gctcgctgcc atcaacttct cgcaagccga ataacggctc
    10501 aaactgctcc gggaattcgt tcagacgctc tgcggtatcg gccatatgtt gaaaagccag
    10561 gttcataaag cgcgtcatct ctttctctga cgcccagcgc ggcacatagt cagccccacc
    10621 ccacacggca accagccact gttccggttc aatctcttgc ggagaactca acaccgccgt
    10681 caataaatcg tccagctccg ccacatcaag gatggcgtgg ttagtgttgt atttggtcag
    10741 aatatcgtcc agccattcca actcactttc gtttaacggt cccgttttca tacgcttttc
    10801 cttgtggatc tcaactcgcc agcacctatc ttacatgccg gtccgtatca gagatacttt
    10861 ttgagtggct ttgccggtga ttaaaaatta aggagggtgt aacgacaagt tgcaggcaca
    10921 aaaaaaccac ccgaaggtgg tttcacgaca ctgcttattg ctttgatttt attcttatct
    10981 ttcccatggt acccggagcg ggacttgaac ccgcacagcg cgaacgccga gggattttaa
    11041 atcccttgtg tctaccgatt ccaccatccg ggctcgggaa gaaagtggag gcgcgttccg
    11101 gagtcgaacc ggactagacg gatttgcaat ccgctacata accgctttgt taacgcgcca
    11161 aattcttcag gcctttcagc cagacatccg cttgacgccg atgtctttta aa
//
LOCUS       DADLFA010000135        10997 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000135 DADLFA010000000
VERSION     DADLFA010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10997
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.136"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(1..105)
                     /locus_tag="JQH65_003008"
                     /pseudo
     CDS             complement(1..105)
                     /locus_tag="JQH65_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858395.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            382..1257
                     /locus_tag="JQH65_003009"
     CDS             382..1257
                     /locus_tag="JQH65_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated protein"
                     /protein_id="HAY6140350.1"
                     /translation="MRKFILISMITLASVSLGACRQNATIKQDAPGQKAFLIDGQIYD
                     LHSGKIISSSELLADLATARHLIIGEKHDNAEHHQIELWLIQNLLIQRPQGSVLLEML
                     TSDQQPRVNQVKCWLKDNPVVRDSRVQELLNWQKGWSWEMYGDIVMQLLRGPYPLLNA
                     NIGREQMLALYKKNEFPKGKKSTAPVVQEALRETIIAMHEGNLESQQLTSMLSVQQQR
                     DRYMARQLLSAPVPSLLIAGGYHASKNMGVPLHMEDLATGTHPVVLMLAEKGMNITVD
                     HADYVWFVAPDTTKR"
     gene            1265..2041
                     /locus_tag="JQH65_003010"
     CDS             1265..2041
                     /locus_tag="JQH65_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004095468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HAY6140351.1"
                     /translation="MMNILHFPQSVKWSSWFICSLLLHGLIFLTFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSDATIEIT
                     RKKKSSDGEKKKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVTFTVNTEGTIVSSFLEISSGTLS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPITFKLKE"
     gene            2210..4471
                     /locus_tag="JQH65_003011"
     CDS             2210..4471
                     /locus_tag="JQH65_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016536327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HAY6140352.1"
                     /translation="MNVIKLAIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVS
                     VGKTTSEQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMS
                     GFGRVNTMVDGITQSFYGTSTSGTTAHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEG
                     INALAGSANMRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGS
                     IGVMAAVSGSSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSA
                     RTYENKFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSA
                     QNRSDALDINNTSRFTVADNELEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPF
                     APSGQQDISALYTGLKVTRGIWEADFNLNYTRNRITGYKPACDSRVICVPQGSYDIDD
                     KEGGFNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAET
                     WQAGFNIDTRDLLVEQDALRFKALAYRSRIQNYIYSESYLVCSGGRKCSMAEVIGNDW
                     EGISDEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTY
                     FGAGDMTELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQ
                     DLPQIPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDESHPANSA
                     RGRTWIFGGDIRF"
     gene            4540..5715
                     /gene="senB"
                     /locus_tag="JQH65_003012"
     CDS             4540..5715
                     /gene="senB"
                     /locus_tag="JQH65_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HAY6140353.1"
                     /translation="MNIFTLSKAPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHLRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSDDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            complement(6088..6276)
                     /locus_tag="JQH65_003013"
                     /pseudo
     CDS             complement(6088..6276)
                     /locus_tag="JQH65_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6240..6524
                     /locus_tag="JQH65_003014"
     CDS             6240..6524
                     /locus_tag="JQH65_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY6140354.1"
                     /translation="MHSTLSPLVPVYPVFITVIADHDKPQPSGCLLESQGSLCPICRQ
                     RITHETGWNVHHKVKKVMGGGEELSNLVLLHPNCHRQLHSCEAGSLKKGL"
     gene            6667..6888
                     /locus_tag="JQH65_003015"
                     /pseudo
     CDS             6667..6888
                     /locus_tag="JQH65_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(6909..6971)
                     /locus_tag="JQH65_003016"
                     /pseudo
     CDS             complement(6909..6971)
                     /locus_tag="JQH65_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            7422..8834
                     /gene="ospC1"
                     /locus_tag="JQH65_003017"
     CDS             7422..8834
                     /gene="ospC1"
                     /locus_tag="JQH65_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HAY6140355.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKDFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISSHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQTLFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(8938..9086)
                     /locus_tag="JQH65_003018"
                     /pseudo
     CDS             complement(8938..9086)
                     /locus_tag="JQH65_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            9162..10859
                     /gene="ospD3"
                     /locus_tag="JQH65_003019"
     CDS             9162..10859
                     /gene="ospD3"
                     /locus_tag="JQH65_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HAY6140356.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGKLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPNNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMGIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
BASE COUNT         3405 a         2330 c         2331 g         2931 t
ORIGIN      
        1 gttgtaatgt gccagccggg aggccgctat cggcggcttc ttcagataac gacccaggta
       61 gcgcgccgta ttcctcccgc cggccatttt cttcgacatg tacatcaggc gcggaactca
      121 tcggggcgcg ggaatgaaat cccattcaga ctccggatag attcaagcaa gcccacttat
      181 cgtcaaaatc ggtgttgcaa aaacgggagt gaccatagat ccgttttcca agcggacccc
      241 ataatatcgg ctggtaaaca tcactcgcta gtgcattctt tttgttggta atgataatta
      301 tttacattta ttctttgatg ttttatcata attaatgttt tatcaataaa tatatggaaa
      361 tatattgttt cagatgggaa tatgagaaag ttcatcctta tttctatgat aacgttagct
      421 agtgttagtc tgggagcctg cagacagaat gccaccataa agcaggatgc gccagggcag
      481 aaagctttcc tgattgacgg gcagatttac gatttgcaca gtggcaaaat tatttctagc
      541 agcgagcttt tggcggatct tgctaccgct cgacatctta ttattggtga aaaacacgat
      601 aatgccgagc atcatcagat tgagctttgg cttattcaaa atctgctcat ccagcgacca
      661 caggggagtg tgttgcttga gatgttgacg tcagaccaac agccccgagt taaccaagtt
      721 aaatgttggc tgaaagataa tcctgtggtt cgagatagcc gtgttcagga actacttaac
      781 tggcaaaaag ggtggtcctg ggagatgtat ggcgacatcg tcatgcagct attacgtggg
      841 ccttacccgc tgctcaatgc caacattggc cgggagcaga tgttggcact gtacaaaaaa
      901 aacgaatttc ctaaaggaaa aaaatccact gcacctgtgg tacaagaagc tctgcgtgaa
      961 accatcatag ctatgcatga aggcaacctt gaaagccagc aactgacgtc aatgctctct
     1021 gttcagcagc agcgggatcg ctacatggcc cgccagcttc tgagtgcccc ggtaccgtcg
     1081 ttgttgattg caggtggtta tcacgcgtca aaaaacatgg gcgtaccgtt acacatggaa
     1141 gatcttgcca ccggcaccca tccagtggtg ttgatgcttg cagaaaaagg aatgaacatc
     1201 acagtagatc atgctgacta cgtgtggttt gtagcgccag atacaacaaa aaggtaaacc
     1261 atggatgatg aatattctcc acttcccaca gtcggtaaaa tggagcagct ggtttatctg
     1321 ttctctactg cttcatggac tgattttcct tacgtttatc tggcgttttt ccgaagttca
     1381 gcccgctatg tctcctgcac cggcgatcat gctgcaatgg gcagaaatta tagaggctcc
     1441 ttcttctcca ctttcgcttc cggtgggaat cgctcaacag gagtccgcag tgacagaaga
     1501 aaagcaacaa actgaggaca gacaacaacg tcctgtcact gaagacagtg atgcaaccat
     1561 tgaaataact aggaagaaaa agtcatccga tggagaaaag aaaaagacac gtccacctcg
     1621 taagataaag gctcaaacca gcgacagcaa ccccacagcc gtttccagta atgctgcacc
     1681 tcaggctctg gtcgaatcgt cccgtattgc tgcgcctttt aacagtgact caacaaagcg
     1741 agataattct gaggcatctt gggaaagcag agtaaaaggc caccttaacc gttataaacg
     1801 ttaccctggc gatgccagaa aacgtgcacg aaccggcaca gccgttgtga cttttaccgt
     1861 aaacacggaa ggcactattg tcagtagttt cttggaaatc tcgtcaggaa ccctttctct
     1921 tgaccgcgaa gctattgctg ttttagaacg cgctcaacca cttcccaaac caccgcctga
     1981 aatattggag ggaggattat ttaaagtaaa aatgccgatt acctttaagt tgaaggagta
     2041 aagggataat tggccgttca agttcattta actgaaattt aattttcatt tttatgctga
     2101 acattttatc aaacttttcc actgtcgaaa aaatcaaaaa gacagtagat cataattaat
     2161 aaacaaatag ttatacttat ctttattaat attatatcca aggaaattta tgaacgttat
     2221 aaaactggct atcggctcag gcatattatt gctcagctgc ggtgcttact cacaatccat
     2281 cagtgaaaaa actaattccg acaaaaaagg agcggcagaa ttcagtccgc tcagcgtttc
     2341 tgtcgggaag acgaccagtg agcaggaagc tctcgagaaa acaggcgcga ccagttcccg
     2401 gacaacggac aaaaacctgc aatcacttga cgcaacagtg cgtagtatgc ctggtactta
     2461 tactcaaata gatcctggtc agggagcaat cagtgtgaat attcgaggca tgagcggatt
     2521 tggtcgtgta aacactatgg tcgatggtat tacccagagt ttttacggaa cctctacctc
     2581 cggaacaacg gcgcatggtt caactaacaa tatggctggc gtacttatag atcctaactt
     2641 actggtagca gttgatgtta cacgcggtga cagcagtggc tctgaaggga tcaacgccct
     2701 tgccggtagt gcaaatatgc gtactattgg cgttgacgat gtaatattta acggtaatac
     2761 atatggcctt cgttcacgtt tctctgtcgg tagtaatggg ctgggacgca gcggaatgat
     2821 cgcccttggt ggaaaaagcg acgcttttac ggatacggga agcattggcg ttatggctgc
     2881 tgtgagtggc agttctgtgt actctaattt ctcaaatggt tctggaatta acagcaaaga
     2941 gtttggttat gataaatata tgaagcagaa ccccaaatcc caactgtata aaatggatat
     3001 cagaccagac gaatttaaca gcttcgaact ttccgctcga acctatgaaa ataaatttac
     3061 acgtcgtgat ataaccagtg acgactatta cattaaatat cattacaccc ctttttctga
     3121 attaattgac tttaacgtaa cggccagtac cagtcgcggt aatcaaaagt atcgtgatgg
     3181 ctcgctgtat actttctaca aaacctcagc gcaaaatcgt tctgacgcgc tggatatcaa
     3241 caataccagc cggttcactg tcgcggacaa tgaactggag tttatgctgg gcagcaaact
     3301 gatgcgtacc cgctatgacc ggaccattca ctcagcggcg ggcgacccga aagcgaatca
     3361 ggaatcgatc gagaacaatc cgttcgcacc ctccggccag caggatattt cagcgctgta
     3421 taccgggctg aaggttacgc gcggcatctg ggaggcagat ttcaatctca actacacacg
     3481 taacaggatc acagggtaca agcccgcctg cgattcacgc gttatctgcg tgccacaggg
     3541 tagctacgat attgacgata aagagggtgg cttcaaccct tcagttcagc tttctgctca
     3601 ggtaacacca tggcttcagc cgttcattgg ctacagcaaa tccatgcgcg ccccgaacat
     3661 ccaggagatg ttcttctcta attcaggagg cgcatccatg aacccattcc tgaagcctga
     3721 acgtgcagaa acctggcagg cgggttttaa cattgatacc agagatttac tggtcgaaca
     3781 ggatgccctg cgctttaagg ctctggcgta ccgcagcagg atccagaact acatctacag
     3841 cgagtcttat ctggtttgtt ctggaggtcg taaatgcagt atggctgagg tgattggcaa
     3901 tgactgggag ggcattagcg atgaatacag cgacaatatg tacatctacg ttaactcggc
     3961 aagcgacgtt attgcaaagg gcttcgaact ggagatggat tatgatgcag gttttgcttt
     4021 tggccgactc tctttcagcc agcagcaaac agaccagcca acctccatcg ccagcaccta
     4081 ctttggcgca ggggatatga ccgaactgcc cagaaaatac atgacgctgg atactggtgt
     4141 tcgcttcttc gataacgcgt tgaccctggg cactatcata aaatacacag gcaaggctcg
     4201 tcgcctgtcg cctgattttg agcaggacga acataccggc gcaataatca aacaggattt
     4261 gccgcagatc ccaacgatta tcgatctcta tggtacttac gagtacaacc gcaacctgac
     4321 actgaaactt tcggtacaaa acctgatgaa cagagattat tcggaggcgc tgaataagct
     4381 caacatgatg ccaggtcttg gtgacgagag ccacccagcc aattccgcgc gtggcagaac
     4441 atggatattt ggcggggaca ttcgtttctg atgacaattt ccgccaggtt cagacaatac
     4501 gttttctctc ttatgtcaat tttattgcag gaacgaaaaa tgaatatttt cactttatcc
     4561 aaagcaccgc tatacttgtt aatttcacta tttttaccca cgatggccat ggctatcgat
     4621 ccacctgaac gcgaactttc gcgatttgcc ctgaaaacga attaccttca gtcccctgat
     4681 gaaggcgtct atgaactggc gtttgataat gccagtaaaa aggtgtttgc agcagtcacc
     4741 gatcgtgtaa atcgtgaagc caataaaggc tatctgtatt cgtttaattc agattcgctg
     4801 aaagtcgaaa ataaatacac gatgccatac cgggcatttt cgctggcgat aaatcaggat
     4861 aaacatcagc tctatatcgg acacacccag tcagcgtccc tgcgtatcag tatgtttgac
     4921 accccaaccg gtaaactggt aagaaccagc gacaggttaa gttttaaagc ggcaaacgct
     4981 gcagattcgc gttttgagca tttgcgccat atggtttaca gccaggattc cgataccctg
     5041 tttgtgagtt atagcaatat gctgaaaacg gccgagggca tgaagcctct gcataagctg
     5101 ttaatgctcg acgggacgac gcttgcctta aaaggcgagg ttaaggatgc ttacaaaggt
     5161 acagcgtatg gtctgacgat ggatgaaaaa acacagaaaa tctacgttgg cggaagagat
     5221 tacatcaacg aaattgatgc gaaaaatcag acgctgctgc gtaccatccc gttgaaagat
     5281 ccgagaccac aaatcacaag tgtgcagaat ctggcggtgg actccgcttc tgaccgtgcc
     5341 tttgtggtgg tattcgacca tgacgatcgt tccggtacaa aagatggcct ctatattttt
     5401 gacttacgcg acggtaaaca gcttggctat gtgcacacag gagccggagc taacgcggtg
     5461 aaatacaatc cgaaatataa cgaactgtat gtcaccaact tcactagcgg caccatcagc
     5521 gtagtggatg ccaccaaata cagcatcacc cgtgaattta acatgccggt ctacccaaac
     5581 cagatggtgt tgtcggacga tatggatacc ctttacattg gcatcaaaga aggctttaac
     5641 cgcgattggg atcctgatgt gtttgtggaa ggagctaaag aacgtattct gagcattgat
     5701 ttgaaaaagt cgtgatcggg ggaaaaaaca tgggataaaa actccgttat tccactaaac
     5761 tggccggact aggaaaagca aacatctctg tcctgccggt acgcctgtta cgccgctcca
     5821 tgaggctgag gaatctccag aaaacgagac aggcacagca ttgtacgatc aactgatttg
     5881 tgccaataat tcaatgaaat cacagctatg cctgttcacg tataggaaat tgaaaaacta
     5941 agcgatacca ctgtccgcgt cttatgcctc gtgcggtaag ctgtttttca catcacgccg
     6001 gtgttttttt ctttctgtgg acggttttaa tactgtaatc ggccgttttt tatacttacc
     6061 ccgattcccg ggcgcactta tcctgcatca ccccgggata tacctcagca gactgatatc
     6121 ccgcgcgttt ttcttcagcc cctccacatt cagtcccgcg atggcccggc ggtataccat
     6181 ccggccgcca cacagcacgc attcgaacgg gtcacgactc aggaactgtt tcaccatttg
     6241 tgcatagcac actttcgcca ctggttccgg tttatcctgt ttttataacg gtaattgcag
     6301 accatgacaa accgcagccg tcaggctgcc tactcgagtc acaaggctct ctgtgtccga
     6361 tatgccggca gcgcattacc catgaaactg gctggaacgt acaccataag gtgaagaaag
     6421 ttatgggggg cggtgaagaa ttatctaacc ttgtcttgtt acatccaaat tgccatcgac
     6481 agttacacag ttgtgaagcc ggctctctca aaaagggctt ataaaggctt gagcggtgta
     6541 ccgggaaacc ggtatgcacc gttcttaggg gagggcagtc cagcaatgaa ctgtccttac
     6601 ccgacaaaca tccaaaacga acggaacgtg ctggaatccg catataaccc gttgtaaagt
     6661 gaggaaatgg acacctcact tgctcatgag aacgcccgcc tgcgggcact gttgcagacg
     6721 caacaggaca ctatccacca gatggctgaa tacaaccgcc tgctctcaca gcggatggcg
     6781 gcttatgctt ccgaaatcaa ccgactgaag gcgctggttg cgaaactgca gcgtatgcag
     6841 ttcggtaaaa gcccagaaaa acttcgcgca aaaaccgaac ggcagattcc attttcacgg
     6901 gcgatctacg ccacacaggc tctgggttgc agtgactgct ccattaaggc gattttgaac
     6961 tcataaggga agtaacaaaa aaactgcaaa agtacaataa ttatatatgg ccaaactaac
     7021 ctctattaaa aaaacatcag tttttcacac ttagttgagc ttcatgaaaa aatcatcgag
     7081 gatttcattg cagaatacac attccactaa ctggaagcac tccataagag gaatcagaga
     7141 aagtatcgtt tttttatagt aaaattcatt gctgttcaat aaaattgata taattattaa
     7201 attatggaaa taatttcaca aaaaagatta acttgagttc ttaagcatcg gattttagat
     7261 attttgaaat atctggcatc acataacatc aaaagtatat aataccctta tctaattaca
     7321 tgcagtatat tgtatatata ctcaaaaatt gtaattcata ccctactata tcttgtgggg
     7381 aaagatatta attaaaactg ttttcatata aggttcattt tatgaatata tcagaaacac
     7441 tgaactcagc aaatacccaa tgcaatatag attctatgga taacagatta catacattgt
     7501 ttccaaaagt gacatcagtg cgaaacgctg cacaacaaac tatgccagat gaaaaaaatt
     7561 taaaagatag tgcaaatatt attaaagatt tctttaggaa aactatagca gcacaaagtt
     7621 atagtagaat gttctctcaa ggctctaact ttaaatcttt aaatatagca attgatgcac
     7681 catcagacgc taaagcctca tttaaggcta ttgagcacct tgacagatta tcgaagcatt
     7741 atatatctga aataagggaa aaacttcatc ctctttctgc agaggaactc aatttgcttt
     7801 cgctaattat taattctgat ttaatcttca gacatcaaag taattctgat ttgtctgata
     7861 aaattttaaa cattaagtca ttcaataaaa ttcagtctga aggaatatgc acaaaacgaa
     7921 acacatacgc tgatgatata aaaaaaatag ctaatcatga ctttgtgttt tttggcgttg
     7981 aaatctctag ccatcagaaa aaacaccccc tgaatacaaa acatcacact gttgattttg
     8041 gtgcaaatgc gtatatcatt gatcatgact ctccatatgg atatatgaca ttaaccgatc
     8101 actttgataa tgctattcca cctgtttttt accatgagca ccaatcattt ttagataaat
     8161 tttcagaggt taataaagaa gttagtcgat acgtacatgg aagtaaagga attatagatg
     8221 taccaatatt caatactaaa gatatgaagt tagggctcgg attatacctg attgacttta
     8281 ttagaaaaag tgaagaccaa agcttcaagg agttttgcta tggaaaaaat cttgcccctg
     8341 tggatctgga tagaatcata aactttgttt ttcagccaga gtaccatata cctaggatgg
     8401 taagtacaga aaacttcaaa aaagttaaga ttagagaaat atccttagag gaggctgtta
     8461 cagcatctaa ttacgaagaa attaacaagc aggtcactaa caaaaaaatt gctctccaga
     8521 ctctttttct ttcgattact aatcaaaaag aggatgtcgc cttatatata ttatctaatt
     8581 ttgagataac tagacaagat gttatttcca taaagcatga gttgtatgat attgagtatc
     8641 tacttagcgc tcataattca agctgtaaag tacttgagta ttttatcaat aagggattgg
     8701 ttgatgtaaa cacaaagttc aaaaaaacta atagtgggga ttgtatgttg gataacgcaa
     8761 taaaatatga gaatgcagaa atgataaaac tattattgaa atatggtgca acatctgaca
     8821 ataaatatat ttaatcaaaa ttgaatatcg tttagcaaaa ggcagtattt gcacaaaata
     8881 tcgatatatt gtttattgtc agtatggctc aatgtgataa tagttggaaa gtttgatggg
     8941 tttcgccccg ttgtagcggt agtcgagctg gaaggtcttc aggcactgct tacagcgata
     9001 gagcagcccc cctgaactgg aatggccgtt ccggataccc cctgagtttc agagtaacgg
     9061 ggacaaacca catcaatctt tgccatcaat catccaaagg gcaaagagta caacaacact
     9121 aagtctgcgt cacaacccat caatgaaagg aatatataca tatgccatca gtaaatttaa
     9181 tcccatcaag gaaaatatgt ttgcaaaata tgataaataa agacaacgtc tctgttgaga
     9241 caatccagtc tctattgcac tcaaaacaat tgccatattt ttctgacaag aggagttttt
     9301 tattaaatct aaattgccaa gttaccgatc actctggaaa acttattgtc tgtcgacact
     9361 tagcttccta ctggatagca cagtttaaca aaagtagtgg tcacgtggat tatcatcact
     9421 ttgcttttcc ggatgaaatt aaaaattatg tttcagtgag tgaagaagaa aaggctatta
     9481 atgtgcctgc tattatttat tttgttgaaa acggttcatg gggagatatt attttttata
     9541 ttttcaatga aatgattttt cattccgaaa aaagcagagc actagaaata agtacatcaa
     9601 atcacaatat ggcattaggc ttgaagatta aagaaactaa aaatgggggg gattttgtca
     9661 ttcagcttta tgatcccaac catacagcaa ctcatttacg agcagagttt aacaaattta
     9721 acttagctaa aataaaaaaa ctgactgtag ataattttct tgatgaaaaa catcagaaat
     9781 gttatggtct tatatccgac ggtatgtcta tatttgtgga cagacatact ccaacaagca
     9841 tgtcctccat aatcagatgg cctaataatt tacttcaccc caaagttatt tatcacgcga
     9901 tgcgtatggg attgactgag ctaatccaaa aagtaacaag agtcgtacaa ctatctgacc
     9961 tttcagacaa tacgttagaa ttacttttgg cagccaaaaa tgacgatggt ttgtcaggat
    10021 tgcttttagc tttacaaaat gggcattcag atacaatctt agcatacgga gaactcctgg
    10081 aaacttctgg acttaacctt gataaaacgg tagaactact aactgcggaa ggaatgggag
    10141 gacgaatatc gggtttatcc caagcacttc aaaatgggca tgcagaaact atcaaaacat
    10201 acggaaggct tctcaagaag agagcaataa atatcgaata caataagctg aaaaatttgc
    10261 tgaccgctta ttattatgat gaagtacaca gacagatacc cggactaatg tttgctcttc
    10321 aaaatggaca tgcagatgct atacgcgcat acggtgagct cattcttagc ccccctctcc
    10381 tcaactcaga ggatattgta aatttgctgg cctcaaggag atatgacaat gttcccggac
    10441 ttctgttagc attgaataat ggacaggctg atgcaatctt agcttatggt gatatcttga
    10501 atgaggcaaa acttaacttg gataaaaaag cagagctgtt agaagcgaaa gattctaatg
    10561 gtttatctgg attgtttgta gccttgcata atggatgtgt agaaacaatt attgcttatg
    10621 ggaaaatact tcacactgca gaccttactc cacatcaggc atcaaaatta ctggcggcag
    10681 aaggcccaaa tggggtatct ggattaatta tagcttttca aaataggaat tttgaggcaa
    10741 taaaaactta tatgggaata ataaaaaatg aaaatattac acctgaagaa atagcagaac
    10801 acttggacaa aaaaaatgga agtgattttc tagaaattat gaagaatata aaaagctgaa
    10861 tattatctcg tggtgataca ctgaatttga ggtaaccggc tcatttaaac cgtctggtct
    10921 gttacctccg gttccacaaa aataatgtcc atcattttga agtggacact atcgtatgga
    10981 ataccggacc tggatca
//
LOCUS       DADLFA010000136        10903 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000136 DADLFA010000000
VERSION     DADLFA010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10903
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.137"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            14..184
                     /locus_tag="JQH65_003020"
     CDS             14..184
                     /locus_tag="JQH65_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001429095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene biosynthesis protein"
                     /protein_id="HAY6140357.1"
                     /translation="MLQEKEYPITIYNIAPFNKNLWSLLKSSQECPPGESKITNKCLH
                     NSCIIKICSICY"
     gene            complement(252..1502)
                     /gene="icd"
                     /locus_tag="JQH65_003021"
     CDS             complement(252..1502)
                     /gene="icd"
                     /locus_tag="JQH65_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HAY6140358.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGEAIIENM"
     gene            1674..2327
                     /gene="rluE"
                     /locus_tag="JQH65_003022"
     CDS             1674..2327
                     /gene="rluE"
                     /locus_tag="JQH65_003022"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HAY6140359.1"
                     /translation="MRQFIISENTMQKTSFRNHLVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYTLDNLANGEWREATE"
     gene            2337..2798
                     /gene="nudJ"
                     /locus_tag="JQH65_003023"
     CDS             2337..2798
                     /gene="nudJ"
                     /locus_tag="JQH65_003023"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HAY6140360.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2852..3958
                     /gene="mnmA"
                     /locus_tag="JQH65_003024"
     CDS             2852..3958
                     /gene="mnmA"
                     /locus_tag="JQH65_003024"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAY6140361.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCSVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3994..4635
                     /gene="hflD"
                     /locus_tag="JQH65_003025"
     CDS             3994..4635
                     /gene="hflD"
                     /locus_tag="JQH65_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HAY6140362.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVPAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4639..6009
                     /gene="purB"
                     /locus_tag="JQH65_003026"
     CDS             4639..6009
                     /gene="purB"
                     /locus_tag="JQH65_003026"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAY6140363.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6177..6848
                     /gene="phoP"
                     /locus_tag="JQH65_003027"
     CDS             6177..6848
                     /gene="phoP"
                     /locus_tag="JQH65_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HAY6140364.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6848..8308
                     /gene="phoQ"
                     /locus_tag="JQH65_003028"
     CDS             6848..8308
                     /gene="phoQ"
                     /locus_tag="JQH65_003028"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HAY6140365.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMQKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            8384..9505
                     /locus_tag="JQH65_003029"
     CDS             8384..9505
                     /locus_tag="JQH65_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAY6140366.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9554..10780)
                     /gene="pepT"
                     /locus_tag="JQH65_003030"
     CDS             complement(9554..10780)
                     /gene="pepT"
                     /locus_tag="JQH65_003030"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAY6140367.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLAGMKGTVERADMH
                     YIIRDFDRKQFEARKCKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
BASE COUNT         2697 a         2787 c         2726 g         2693 t
ORIGIN      
        1 taagcttggt attatccttc aggaaaaaga atatcctatt actatttata acattgcacc
       61 attcaacaag aatttatgga gtttactcaa aagcagtcaa gagtgtcctc caggagaaag
      121 caaaataaca aataaatgtt tacataatag ttgcattata aaaatatgct cgatatgcta
      181 ttaataatat ccacataaat aaaacaacgg gcgtgttata cgcccgtttc aatatttaac
      241 tcatggagag attacatgtt ttcgatgatc gcttcaccaa actctgaaca tttcagcagc
      301 ttagcgcctt ccatcagacg ttcaaagtca taagttacgg ttttcgcgtt gatagcacct
      361 tccatacctt taacaattaa atcagccgct tcagtccacc ccatatggcg cagcatcatc
      421 tcagcggaga ggatgataga gcctggattt actttgtcct gaccggcgta tttcggcgca
      481 gtaccgtggg tggcttcaaa cagggcgcat tcgtcaccga tgtttgctcc aggagcgata
      541 ccgataccgc caacctgcgc tgccagggcg tcagaaatgt agtcaccgtt caggttcata
      601 caggcgataa catcatattc agccggacgc agcaggatct gttgcaggaa tgcatcagca
      661 atcacgtctt taatgacgat ctctttgccg gtgttcgggt ttttaacttt cagccacggg
      721 ccgccgtcga tcagttcacc gccaaactct tcacgcgcca gctggtagcc ccagtcttta
      781 aacgcgcctt cggtgaactt catgatgttg cctttgtgca ccagagtcac agagtcacga
      841 tcgttagcaa ttgcgtattc gatcgctgca cgaaccagac gtttggtgcc ttcttccgaa
      901 cacggcttaa taccgatacc acaatgttcc gggaagcgaa ttttcttcac ccccatctct
      961 tcacgcagga atttaatcac tttctcggcg tcggcagagt ctgctttcca ttcgataccc
     1021 gcataaatgt cttccgagtt ttcacggaag ataaccatat cggtcagttc agggtgttta
     1081 accgggcttg gagtgccctg atagtaacgt accggacgca ggcagatgta gagatccagt
     1141 tcctggcgca gggcaacgtt cagagagcga ataccgccac caacaggagt ggtcagcgga
     1201 cctttaatgg caacgcgata ttcacgaatc agatcaaggg tttcagcagg cagccagaca
     1261 tcctgaccat aaacctgtgt ggatttttca ccggtgtaaa tttccatcca ggagatttta
     1321 cgctcgcctt tataggcttt ctcgactgca gcgtcgacca ctttcagcat ggctggggtt
     1381 acatctacac cgattccatc accttcaatg taagggataa tcggattttc aggaacgttg
     1441 agtttgccgt tttgcagggt gatcttcttg ccttgtgccg gaacaactac tttactttcc
     1501 attcacctct ccttcgagcg ctactggttt gctcgtctgc caccacgttg catatgcgtt
     1561 tgcgtcctgc gatacggatg ctttagagca attttttgtt aatgatttgt aattggcttg
     1621 tcaatactac cctattgttt gccgtcatga aagatgcgcg ttattaggct ataatgcggc
     1681 aattcataat ctctgaaaat accatgcaaa aaacttcttt tagaaatcac ctggttaagc
     1741 gattcagctc gcaacgttct accaggcgta aacctgaaaa ccagcccacg cgtgtgatcc
     1801 tgttcaataa accctacgat gttcttccgc agttcaccga tgaagccgga cgcaaaacat
     1861 taaaagaatt catcccggtt cagggcgttt atgcagcagg tcgtcttgac cgcgatagcg
     1921 aagggttact ggtgctgacc aataacggcg cgttgcaggc gcgtttaacc cagccgggca
     1981 aacgcaccgg gaaaatctat tatgtgcagg tggaaggtat tcccacacaa gacgcacttg
     2041 aagccttgcg caatggcgta accttaaatg atggccctac cctgcccgcc ggcgcggagc
     2101 tggttgacga accagcgtgg ttatggccgc ggaatccacc aattcgtgaa cgcaaaagta
     2161 ttcccaccag ctggctgaag atcaccttat atgaaggacg taatcgccag gtgcgccgca
     2221 tgaccgccca tgttggtttc cccacgctgc gactgattcg ttatgcgatg ggtgattaca
     2281 ctttggataa ccttgccaat ggcgaatggc gagaagcaac agaataagga tgcacaatgt
     2341 ttaaaccaca cgttaccgtt gcttgcgtag tgcacgcaga gggtaaattt ttagtcgttg
     2401 aagagacgat taatggtaaa gcgttatgga accaacctgc cgggcatctg gaagccgatg
     2461 aaaccttagt ggaagctgcc gcccgtgagc tatgggaaga aaccggcatc agcgcgcaac
     2521 cgcaacactt tattcgtatg catcagtgga ttgcgccaga taaaacgccg tttttgcgtt
     2581 tcctctttgc cattgagctt gagcaaatat gcccgaccca gcctcatgac agcgatatcg
     2641 actgctgtcg ttgggtcagc gccgaagaaa ttttaaaggc gtcaaatctt cgttcgccgc
     2701 tggtggcgga aagtatccgt tgttatcaaa gcgggcaacg ctatccgctg gagatgattg
     2761 gcgattttaa ctggcctttt acaaagggtg tcatctaaaa ggctgcgtga tagaatacgc
     2821 cgccttgaag ttcaatgtcg tgagtgatcc aatgtctgaa accgcaaaaa aagtaatcgt
     2881 cggcatgtcc ggcggtgtcg attcctccgt ttctgcctgg ctgttgcaac aacagggata
     2941 tcaggttgaa ggcctgttta tgaagaactg ggaagaagac gacggtgagg aatattgcac
     3001 agcggcagcg gatctggctg atgcccaagc tgtctgcgac aagctcggca ttgaactgca
     3061 caccgttaac tttgctgccg agtactggga caacgtcttc gaactgttcc ttgccgaata
     3121 taaagccggt cgcacgccga atccggatat tctgtgcaac aaagagatca aatttaaagc
     3181 tttcctcgaa tttgccgccg aagatttagg tgccgattat atcgctaccg gtcattacgt
     3241 acgtcgggcc gatgtcgatg gcaagagccg cctgctgcgt ggtctggaca gcaataaaga
     3301 ccagagctac ttcctttata cgctcagcca tgagcagatt gcgcaaagcc tgttcccggt
     3361 cggcgaactg gaaaaaccgc aggtgcgtaa gattgctgaa gatcttggtc tggtcaccgc
     3421 gaagaaaaaa gactctaccg gcatttgctt cattggcgaa cgtaaattcc gcgagttcct
     3481 gggccgttat ctcccggcgc aaccgggcaa aatcattacc gtcgatggcg atgaaattgg
     3541 tgagcaccag gggctgatgt atcacactct cggtcagcgt aaaggtctgg gtatcggtgg
     3601 caccaaagaa ggcaccgaag aaccgtggta tgtggtggac aaagacgtcg aaaacaacat
     3661 tctggttgtt gcgcagggcc atgaacaccc gcggctgatg tctgtcgggt tgattgccca
     3721 gcagttgcac tgggtcgatc gcgaaccatt caccggtact atgcgttgct cggtaaaaac
     3781 ccgctatcgc cagaccgata tcccttgcac cgtcaaggcg ctggacgatg atcgcattga
     3841 agtgattttc gatgaaccgg ttgccgccgt gacgccgggc cagtctgccg tcttctataa
     3901 cggtgaagtg tgcctcggtg gcggtattat tgagcagcgt ctgccgctgc cggtctgatt
     3961 attatcttta cttaaaacaa ggaagcagtg aacgtggcaa agaattacta tgacatcacc
     4021 ctcgccctgg ccggtatttg tcagtcggca cgcctggtgc aacaactcgc tcaccagggg
     4081 cattgtgatg ccgatgcgct acacgtctca ctcaacagta ttattgatat gaaccccagc
     4141 tcgacgctgg cggtttttgg cggtagcgaa gccaacctgc gcgtcgggct ggaaaccctg
     4201 ctcggcgtgc tcaatgccag cagtcgccag ggcttaaacg ccgaattaac ccgctacaca
     4261 ctcagcttga tggtgcttga gcgcaaactc tcctcagcga aaggcgcgct cgacactctg
     4321 ggcaaccgga tcaacggcct gcaacgccag ctcgaacact tcgatttaca gtccgaaacg
     4381 ctgatgagcg cgatggctgc tatctatgtt gatgtgatca gcccgcttgg cccgcgcatt
     4441 caggtcaccg gttcccctgc tgtactgcaa agcccacaag tgccggcgaa agttcgcgca
     4501 accctgctgg caggcattcg cgccgccgtg ctctggcacc aggtcggcgg cggacgtctg
     4561 caactgatgt tttctcgtaa tcgcctgacc actcaggcaa aacaaattct tgctcattta
     4621 accccggagt tgtgatctat ggaattatcc tcactgaccg ccgtttcccc tgtcgatgga
     4681 cgctacggcg ataaagtcag cgcgctgcgc gggattttca gcgaatatgg tttgctgaaa
     4741 ttccgtgtac aagttgaagt acgttggctg caaaaactgg ccgcgcacgc agcgatcaag
     4801 gaagttcctg cttttgctgc cgacgcaatc ggttaccttg atgcaattgt cgccaatttc
     4861 agcgaagaag atgccgcacg catcaaaacc atcgagcgta ccactaacca cgacgttaaa
     4921 gcggttgagt atttcctgaa agaaaaagtg gcggagatcc cggaactgca cgcggtttct
     4981 gaattcatcc actttgcctg tacttcggaa gatatcaata acctctccca cgcattaatg
     5041 ctgaaaaccg cgcgtgatga agtgatcctg ccgtactggc gtcaactgat tgatggcatt
     5101 aaagatctcg ccgttcagta ccgcgatatc ccgctgctgt ctcgtaccca cggtcagcca
     5161 gccacgccgt caaccatcgg taaagagatg gcaaacgtcg cctaccgtat ggagcgccag
     5221 taccgccagc ttaaccaggt ggagatcctc ggcaaaatca acggcgcggt cggtaactat
     5281 aacgcccaca tcgccgctta cccggaagtt gactggcatc agttcagcga agagttcgtc
     5341 acctcgctgg gtattcagtg gaacccgtac actacccaga ttgaaccgca cgactacatt
     5401 gccgaactgt ttgattgcgt tgcgcgcttc aacaccattc tgatcgactt tgaccgtgac
     5461 gtctggggtt atatcgccct taaccacttc aaacagaaaa ccattgctgg tgagattggt
     5521 tcttccacca tgccgcataa agttaacccg atcgacttcg agaactccga agggaatctg
     5581 ggcctttcca acgcggtatt acagcatctg gcaagcaaac tgccggtttc ccgctggcag
     5641 cgtgacctga ccgactccac cgtgctgcgt aacctcggcg tgggtatcgg ttatgcgctg
     5701 attgcgtatc aatccaccct gaaaggcgtg agcaaactgg aagtgaaccg tgaccatctg
     5761 ctggatgagc tggatcacaa ctgggaagtg ctggctgagc caatccagac agttatgcgt
     5821 cgctatggca tcgaaaaacc gtacgagaag ctgaaagagc tgactcgtgg taagcgcgtt
     5881 gacgccgaag gcatgaagca gtttatcgac ggtctggcgt tgccagaaga agagaaagcc
     5941 cgcctgaaag cgatgacgcc ggcaaactac attggtcgcg ccatcaccat ggttgatgag
     6001 ctgaaataaa ctcgtatcag tgccggatgg cgatgctgtc cggcctgctt attaagatta
     6061 tccgcttttt attttttcac tttacctccc ctccccgctg gtttatttaa tgtttacccc
     6121 cataaccaca taatcgcgtt acactatttt aataattaag acagggagaa ataaaaatgc
     6181 gcgtactggt tgttgaagac aatgcgttgt tacgtcacca ccttaaagtt cagattcagg
     6241 atgctggtca tcaggtcgat gatgcagaag atgccaaaga agccgattat tatctcaatg
     6301 aacatttacc ggatattgcg attgtcgatc tcggattgcc agacgaggac ggtctgtcac
     6361 tgattcgccg ctggcgtagc aatgatgttt cactgccgat tctggtatta accgcccgtg
     6421 aaagctggca ggacaaagtc gaagtattaa gtgctggtgc tgatgattac gtgactaaac
     6481 catttcatat tgaagaggtg atggcgcgaa tgcaggcatt aatgcggcgt aatagcggtc
     6541 tggcttcaca ggtcatttcg ctaccaccgt ttcaggttga tctctctcgc cgtgaattat
     6601 ctattaatga cgaagtgatc aaactgaccg cgttcgaata caccattatg gaaacgttga
     6661 tacgcaataa tggcaaagtg gtgagcaaag attcgttaat gctccaactc tatccggatg
     6721 cggagctgcg ggaaagccat accattgatg tactgatggg acgtctgcgc aaaaaaattc
     6781 aggcacaata tccgcaagaa gtgattacta ccgttcgcgg ccagggctat ttgttcgaat
     6841 tgcgctgatg aaaaaattac tgcgtctttt tttcccgctc tcgctgcggg tacgttttct
     6901 gttggcaacg gctgcggtag tactggtgct ttcgcttgcc tacggaatgg tcgcgctgat
     6961 cggttatagc gtcagtttcg ataaaactac gtttcggctg ttacgtggcg agagcaatct
     7021 gttctatacc cttgcgaagt gggaaaacaa taagttgcat gtcgagttac ccgaaaatat
     7081 cgacaagcaa agccccacca tgacgctaat ttatgatgag aacgggcagc ttttatgggc
     7141 gcaacgtgac gtgccctggc tgatgaagat gatccagcct gactggctga aatcgaatgg
     7201 ttttcatgaa attgaagcgg atgttaacga taccagcctc ttgctgagtg gagatcattc
     7261 gatacagcaa cagttgcagg aagtgcgaga agatgatgac gacgcggaga tgacccactc
     7321 ggtggcagta aacgtctacc cggcaacatc gcggatgcaa aaattaacca ttgtggtggt
     7381 ggataccatt ccggtggagc taaaaagttc ctatatggtc tggagctggt ttatctatgt
     7441 gctctcagcc aatctgctgt tagtgatccc gctgctgtgg gtcgccgcct ggtggagttt
     7501 acgccccatc gaagccctgg caaaagaagt ccgcgaactg gaagaacata accgcgaatt
     7561 gctcaatcca gccacaacgc gagaactgac cagtctggta cgaaacctga accgattgtt
     7621 aaaaagtgaa cgcgaacgtt acgacaaata ccgtacgacg ctcaccgacc tgacccatag
     7681 tctgaaaacg ccactggcgg tgctgcaaag tacgctgcgt tctctgcgta gtgaaaagat
     7741 gagcgtcagt gatgctgagc cggtaatgct ggagcaaatc agccgcattt cacagcaaat
     7801 tggctactac ctgcatcgtg ccagtatgcg cggcgggaca ttgctcagcc gcgagctgca
     7861 tccggtcgcc ccactgctgg acaatctcac ctcagcgctg aacaaagtgt atcaacgcaa
     7921 aggggtcaat atctctctcg atatttcgcc agagatcagc tttgtcggtg agcagaacga
     7981 ttttgtcgag gtgatgggca acgtgctgga taatgcctgt aaatattgcc tcgagtttgt
     8041 cgaaatttct gcaaggcaaa ccgacgagca tctctatatt gtggtcgagg atgatggccc
     8101 cggtattcca ttaagcaagc gagaggtcat tttcgaccgt ggtcaacggg ttgatacttt
     8161 acgccctggg caaggtgtag ggctggcggt agcccgcgaa atcaccgagc aatatgaggg
     8221 taaaatcgtc gccggagaga gcatgctggg cggtgcgcgg atggaggtga tttttggtcg
     8281 ccagcattct gcgccgaaag atgaataaat atgtccatac ttcacgcatt acgttaagca
     8341 tccgttataa tcggttgcag ataccagcct gtggatgctt aacatggaat accaactcac
     8401 tcttaactgg cccgattttc ttgaacgtca ctggcagaaa cgcccggtgg tgttaaaacg
     8461 cggctttaat aattttattg acccgatctc tccagacgag ttggcgggtc tggcgatgga
     8521 aagcgaagtt gacagtcgac tggtcagtca ccaggatggc aaatggcagg tcagccacgg
     8581 cccgttcgaa agctacgatc atctcggtga aaccaactgg tcattactgg tacaggcagt
     8641 gaaccactgg catgagccga ccgccgcgct gatgcgaccg ttccgtgaac taccggactg
     8701 gcgtattgat gatctgatga tttctttttc tgtacctggc ggcggcgtcg gcccgcatct
     8761 cgatcagtac gacgtgttta tcattcaggg taccggacgt cgtcgctggc gagtgggcga
     8821 aaagctgcaa atgaaacagc actgcccaca tccggatctg ttacaggtcg atccgttcga
     8881 agccatcatc gatgaagagc tggagcctgg tgatattctt tatattccgc caggattccc
     8941 gcatgaaggc tacgcgctgg aaaatgcgat gaactattcc gtgggctttc gcgcgccaaa
     9001 tacgcgggaa ctgattagtg gatttgccga ttatgtgctg caacgtgaac tgggcggcaa
     9061 ctactacagc gatcctgatg ttccacctcg cgctcatcct gcggatgttc tgcctcaaga
     9121 gatggataaa ctgcgtgaga tgatgctcga attgatcaac cagccggaac actttaagca
     9181 atggtttggc gagtttatat cccagtcacg tcatgaactg gatatcgcgc cgccggaacc
     9241 gccttatcag ccagatgaaa tctacgatgc gctgaaacaa ggtgatgtgc tggtgcgcct
     9301 gggtggtctg cgcgtattgc gcattggcga cgacgtgtat gccaatggtg agaagatcga
     9361 ttccccgcac cgtccggcac tggatgcact cgccagcaac attgcgctga ctgcggagaa
     9421 ttttggcgat gcgctggaag atccgtcatt cctcgcgatg ctcgcggcgc tggtcaatag
     9481 cgggtattgg ttcttcgaag ggtaagtttg gatttaagcc ggatgcggca tcacatgccg
     9541 catccctttt cgcttacttc cgttgcgccg ttaactcggc aatacggacg atcacctgca
     9601 ccgctttttc cataccttcc agagtcacaa actcatgctt accatgatag ttgtaaccgc
     9661 cagtgaacaa gttcgggcac ggtaatccca taaacgacaa ctgcgcgccg tcggtaccgc
     9721 cgcggatcgg tttcagttcc ggttcaatat cgcagtcacg cattgcctgc tgggcgatat
     9781 cgagaatatg cggatgctca accactttct cgcgcatatt gtagtaactg tcttcaatca
     9841 ccagttcaat gtagcaatca ggatgtaacc ctttgcccac ttttttggcg atctccatca
     9901 ttttacattt acgcgcttca aactgtttac ggtcgaaatc acggatgatg tagtgcatat
     9961 cggcccgttc aacggtgcct ttcatgcccg ccaggtgata gaaaccttca tagccttctg
    10021 tcatttccgg gctttcatcc gccggaactt ccgcatgaat acgtgccgcc agcgacagcg
    10081 catttaccat cactcctttc gccgtgcccg gatgaacatt gttaccgaca attttgatat
    10141 tgaccgatgc ggcgttgaag ttttcaaact ccagttcgcc tacgccacca ccgtcaacag
    10201 tgtaagccca gcgggcatcg aaggcatcaa catcaaaatg tttcgcccct ttgcccactt
    10261 cttcatccgg ggtaaaggcg acgcgaatat caccatgcgg aatgtttttc tgttgcaata
    10321 ccgccagcgc ggtcatgatt tctgcaatac ctgctttgtc atcggcacct aacaaggttt
    10381 taccatcggt ggtaatcaat gtctgaccca gtagctgatg cagcaccggg aacataaccg
    10441 gtgataaaac ttcatcgccg atacccagcg caatatcgcc accgcgatag ttttcaacaa
    10501 tttgcggatt aacatttttg ccgctgcaat ccggtgaggt atccacatga gaaataaagc
    10561 caatcgccgg gatatcgcca gggacgttag ccggtaacgt cgccatcaaa gtgcccttct
    10621 cacttaaggt cacattgata agccccattt cttcgagctg ctctttcagc agatgcaata
    10681 acttccattg gccttccgtg ctgggaacct gtctcacccc tgcttttgat tgggtatcca
    10741 gagacacgta gttcaaaaat cgctcaagta gtttatccat gtagtcaccc tcactttttg
    10801 tgacaacatt attaagaagc aagaaaagac aaatattgcg tcaggtcact tttacccctg
    10861 caagcgggaa tatttatcag cattacctta atgaatataa agc
//
LOCUS       DADLFA010000137        10897 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000137 DADLFA010000000
VERSION     DADLFA010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10897
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.138"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(228..2087)
                     /gene="gss"
                     /locus_tag="JQH65_003031"
     CDS             complement(228..2087)
                     /gene="gss"
                     /locus_tag="JQH65_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HAY6140368.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2292..3158
                     /gene="yghU"
                     /locus_tag="JQH65_003032"
     CDS             2292..3158
                     /gene="yghU"
                     /locus_tag="JQH65_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HAY6140369.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPICVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3281..3529)
                     /gene="hybG"
                     /locus_tag="JQH65_003033"
     CDS             complement(3281..3529)
                     /gene="hybG"
                     /locus_tag="JQH65_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HAY6140370.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3542..3883)
                     /gene="hypA"
                     /locus_tag="JQH65_003034"
     CDS             complement(3542..3883)
                     /gene="hypA"
                     /locus_tag="JQH65_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HAY6140371.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3876..4364)
                     /gene="hybE"
                     /locus_tag="JQH65_003035"
     CDS             complement(3876..4364)
                     /gene="hybE"
                     /locus_tag="JQH65_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HAY6140372.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4357..4851)
                     /locus_tag="JQH65_003036"
     CDS             complement(4357..4851)
                     /locus_tag="JQH65_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HAY6140373.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4851..6554)
                     /gene="hybC"
                     /locus_tag="JQH65_003037"
     CDS             complement(4851..6554)
                     /gene="hybC"
                     /locus_tag="JQH65_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HAY6140374.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6551..7729)
                     /gene="hybB"
                     /locus_tag="JQH65_003038"
     CDS             complement(6551..7729)
                     /gene="hybB"
                     /locus_tag="JQH65_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HAY6140375.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTREELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7719..8705)
                     /gene="hybA"
                     /locus_tag="JQH65_003039"
     CDS             complement(7719..8705)
                     /gene="hybA"
                     /locus_tag="JQH65_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HAY6140376.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8708..9826)
                     /gene="hybO"
                     /locus_tag="JQH65_003040"
     CDS             complement(8708..9826)
                     /gene="hybO"
                     /locus_tag="JQH65_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HAY6140377.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(10015..10302)
                     /locus_tag="JQH65_003041"
     CDS             complement(10015..10302)
                     /locus_tag="JQH65_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HAY6140378.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10421..10897)
                     /locus_tag="JQH65_003042"
                     /pseudo
     CDS             complement(10421..10897)
                     /locus_tag="JQH65_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
BASE COUNT         2660 a         2989 c         2691 g         2557 t
ORIGIN      
        1 caaagtgaaa tatcattttt taacgataaa aagagtgtta tttcttgtgt atcagacaat
       61 aagcattcat tcaaaacctt tagaaataat caaaacacag gtattccact ggttaatata
      121 ttcacatatg aaatgaataa atattagaac atataaatat tgaatatttt gattaatccc
      181 cgtactgatt attcttcata atcagtacgg ggttgctaca acatgtatta ctttttcacc
      241 acaattaacg gttcaatatc actctctttt ttgatgacca gtgattcatc accgcgcaaa
      301 cacgtcccac cgtagttgcc gcccacggtg aaggtacata cctgaatgta tttaccgtcc
      361 actttcggca aacaccacag ttgctgatag atgtttttct gctcggcaaa tttaccgctg
      421 gttttgtcca gcacctcttc atgatggctg accaggtcga tattgctacc acagcgaccg
      481 gcaatcggtt tcactgcgta acctgttttc accaattcat cattaacagt gaaatcggta
      541 tccagcaggt aacgatggtg cgggaacagc gaccagagga tcggcagaat cgctttgttg
      601 ccggggatca ccgtccacag cggctcaaag actagcactt ccgggcgcag caatacgtcg
      661 ataagacgca cttcgttttg cggatgaccg gtacggattg gcaccgcagc aaactcacgg
      721 tcgctaactt cacgaatctg atcaaacgcg gtttcccacg cccaggtttt ccacacgcag
      781 ttaaccagtc gcccttcccc atcaatcagt tgcccggcag catcccagcc cagttcatcc
      841 aatccacgca agatacgcgt ttcaaagccc gcctggtgca gcgcctgctc cataaactgc
      901 gcgtgatagt tttcctcgat atctttgtcc tgcatgatat ggacaaacgg acgtgcacga
      961 ctgtgtttcc aggcaccagc caattcgtta atcagccctt ccgccggatt gaagccgttg
     1021 cctttatagc cctgctccgc ccaacgttcg aggatcaagc ccgcttcggt atgacaggag
     1081 gcggagtcgg cgttgtactc gtaaaccttc aggccacgct catccatgca gaaatccata
     1141 cgaccagtga tcatatggtg acggcgacgc tgccaggaga gacgcaaacg tggccagagg
     1201 attttcggaa tgtcgaacag cgccagcagg ttgtcatctt tcagtacctt gtcggttgcg
     1261 tgaagataca tcaggtgcag ctcgttggtg gctttaatta gctcctgctc ggcactctcg
     1321 gtaatggtgt agtaatgata aggatcctga ttgatcacct gaccgttggc ctgcacatag
     1381 gcgttttgca gcggatcttt ttcatccagc catttaccgt caaactggcc tttgttttcc
     1441 aggcgcgctc cgctgatttt cagcagctcg cctgcaattt ccggctgcgg taagctgtat
     1501 tcagtatctt ccgtctggat catccagccc agaatggtgg tgtcatcaaa agtgtctttc
     1561 agggtatagc agccgttttc gaccaccatc tccagctcac gcgtccactg ttgcccttgc
     1621 ggcaacgggg aatgaatcac gttctgttcc gcaatacgga ctttgttgcc atgcaattgg
     1681 gtaatgatgg cgacatggcc agtgtcttta aattcaccgc ctttatccca gataagaagc
     1741 gcacccgcga ccggcgcacg cggcgagccg ttaggaaatg cctgcaatgg caggatgttg
     1801 tcattaacca cttctcgcag gaagcgcagc gagaaaatct cccacgccat acccacgtca
     1861 gtaaagacca caccgtaatt cagaaagaga aaacggcgag caaattcaac gcattgccac
     1921 ttgtggccca tatattcgtc gtcgatatag ctacggaata cggcgtcatc ttcgtattcc
     1981 tgcggatcga gagaactgta atctgaagag tagattgcta ccccacctgg ggcgtagccc
     2041 aataatgtcc cgaacggggc atcctggctg gtcgttcctt tgctcatcac tttaccttta
     2101 acaatacaac ctaagcagtt ggcgtaattg ttgtgctctg attacctgct cagcgacatt
     2161 aaccggacag aggtcagact aatcatacac ctcatcgcag cggctggcgc aaagggttaa
     2221 aaaattcaca ttctgtacag caagtgacct gccacactgc ttcaacacta ccactcagaa
     2281 ggcaactcac tatgacagac aatacttatc agcccgcgaa agtctggacg tgggataaat
     2341 ccgctggcgg cgcgttcgcc aatatcaatc gcccggtttc tggtccgacg catgaaaaaa
     2401 cgctgcccgt tggcaaacac ccattgcaac tttattcgct gggaacgccg aacggtcaga
     2461 aagtaacgat tatgcttgag gagctgctgg cgctgggcgt tactggtgca gagtacgacg
     2521 cctggctgat tcgtattggc gatggcgatc aattctccag cggctttgtc gaagtgaacc
     2581 caaactcgaa gatcccggcg ctgcgcgatc atacgcataa tccgccgatc tgtgtgtttg
     2641 aatctggttc gatcctgctt tatctggcgg agaaatttgg ctacttcctg ccgcaggatt
     2701 tggcaaagcg tactgaaacg atgaactggc tgttctggtt acagggcgcg gcaccgttcc
     2761 tcggcggtgg ttttggtcac ttttaccatt acgcaccggt aaagattgag tacgccatca
     2821 accgctttac catggaagcc aaacgtctgc tcgacgtgct ggataagcaa ctggcgcagc
     2881 ataagtttgt tgcgggcgat gagtacacca ttgcggatat ggcgatttgg ccgtggtttg
     2941 gcaacgtggt gttaggtggt gtgtatgatg ccgctgagtt tcttgatgcg ggcagttata
     3001 agcatgtaca acgctgggcg aaagaagtag gcgaacgtcc ggcggtgaaa cgtgggcgta
     3061 ttgttaaccg caccaacgga ccgctgaatg agcagttgca tgagcgccat gacgccagtg
     3121 atttcgagac gaatacggaa gataagcgtc aggggtaagg gttggtgttc gtcgcagcaa
     3181 gccatccagg ccggataagg cgttcacgcc gcatccggca atcgtgcaca atgcctgatg
     3241 cgacgctgcc gcgtcttatc aggcctacga aagctaatca tcacgcgctg gtaatgtcgt
     3301 aatccatttg gcgcagtgcg tctaatgtgg ctttggcttc atcttcgtcg atgatgctca
     3361 tggcaaatcc gacgtgtacc agcacccact ggcccagtag atcggcaggg ttaccttcac
     3421 aaatcagggc gatattcacg tcgcgcttga taccacatac ttcaacctgc gcaagctggt
     3481 gaatatcttc accgacagcc agcacctggc ctggaacgcc aatacacatt attaactccg
     3541 gttattcaac ttcaatactt ttgacgatca gcgaatcgcc ggtatcgaca cgcaaccgct
     3601 cgccgtgaca gagcggacac tgcgcatcgt gctgatgaat ctccaccacc tggctgcaat
     3661 cccagcacca ggcctgggcg ggtttataga cgatatgtaa atcgcaccct tgcgccaccg
     3721 ttccgtggca gacaatttca aaactaaaac ggacggcgct ctcctcaacg caggagagcg
     3781 cgccaatttc cagccacacg gcggtgacgc gcttaacatc gtgctgctcc gcctgccgtt
     3841 ggataatttc aacggcgctc tggcaaagcg acaactcatg cattttcgcc actcctgcga
     3901 ccaaacagca gggcgcgacg ccctgcgtgt ggtacatccg gattcgtgac tggcagcgaa
     3961 aggatcattc gtgcgcagtc atccgtcagg cgttggccct cttcaatcga catactgtgc
     4021 gaaagcggtg acatcagcga acaggagagg tattgcgaaa caccgtccag ttcgccaacg
     4081 gtaaaggtca tggtgccata cggcagttgc agaccaattt tttcactcac tttgcgcagc
     4141 ggccagagtt gatccgggcc ggggaagata actgcactca gcatccacgg ggtgatcaca
     4201 caccccgtcc actgaccttc gaacagcgta aaatcagaaa catacaccgg cattgaagga
     4261 tgcagaaaag agagatcgtg catcgaacgc cgggcaattt cttcaaacgc tgcctgtact
     4321 tgcgccttcg gggaggtctg gaaacctgct atctcttcag tcatgaatcg cctcccgtgg
     4381 gatggcttcc acgcctgatt cgcgcagcgc agccagaacc tgctcaagcg caggttcaat
     4441 cattgcttca accgtcggcg ttaagccgat gtgtggctcc agcgattccg ggatcacgcc
     4501 gaccagggtc agctttttcg gaaactcacc ggtgaagcgc agggccgaca atacgtcggc
     4561 caggccaagc tgatgcggag agattttgtt ggtaaacaac gccggaactt cttcatcccg
     4621 caggatcatc atcgttcccg gcgtgttctt tttcgacaca atggcatccg caataatcag
     4681 atgatcgcga tttgccatgt cgccaagcag ctccattccc gccgtgccgc catcgaggat
     4741 ctcaacataa tccggcagaa tgtatcgttg ctctaacgct tcgacaatcc gcacaccgat
     4801 ggcttcatcg gtcagcaaaa tattgccgac ccctaagact aaaatacgca ttacagaacc
     4861 ttcactgaaa ccacttcgtt gccgtcggca tccactacgt gtaccgcaca ggccatgcac
     4921 gggtcgaagg agtgaatggt acgcaccact tccagcggtt tattcggatc agcaaccggt
     4981 gtacccacca gcgactgctc gtaaggaccg acgtcatcat tgaagttacg cggaccagag
     5041 ttccaggttg atggaacaac tgcctggtag ttactgataa taccgtcttt aataaccatc
     5101 cagtgagaga gcataccgcg cggcgcttcg aggaagccaa cacctttgaa ctcacccgtt
     5161 gccggaatgt tcggtttcac aaaggtggtg tgatcgcctt tgccgatatt ggtgatcagt
     5221 gcactgtatt gattttgcag gatatcctgc aattcgcagc agtgaacggt acgaccaata
     5281 atacggccca gcgtggagtg cagctgcgcc acttccagcg tgttgccagt cagtttctga
     5341 taaatcgcaa cgatttcatt cagtttgttc tgggtagatt cgcgacctgc cgccagtttc
     5401 accagcatat tagccagcgg acccacttct accgttttgc cgtagaaagt cggtgatttc
     5461 acccaggaat atttcccgtc gtcagaccaa ccatcataag ccggaatggt ggtaccttcc
     5521 cacggtgcct gcggcgcttc gtctttatac caggagtgct tcgcgctttc ctgaatgcct
     5581 ttgatcaggt attcatcgga atgagaagtg atcggacgat acgaggacag atccgcattc
     5641 tcaatgtagc cgcccgggaa caggaagctg ccgtttttac tgtcggtcgg gaattccggc
     5701 acgctcaggt agttcaccgc acctttaccg cgcgtcagcc attccgggta gaacgcggca
     5761 ataactgcgg tatcaacctt ataaacctgt tcaacaaagt cgctcagctt gtcgatgaaa
     5821 gacttgatgt acatcaggcg ctcaaggttc agcacgccca aaccgtcgag gttgatcggg
     5881 ttcgcgacac cacctaccgc caggttctga atgtgcggcg ttttaccgcc cagcagcgcc
     5941 acgacgcggt tagcgtcacg ctggcactcc agcgcctgca ggtagtgcgc taccgcaatc
     6001 aggttcactt ccggcggcag tttcatcgcc gggtgacccc agtagccatt ggcgaaaata
     6061 cccaactgac cgctggcaac cagatctttg atcttgttct gaactttagt gaactcttcc
     6121 ggactgttca gatgccaggt cgaaacgcct ttcagcattt cggaggcttt ggttgggtca
     6181 gcttgcagtg cagaagtgat gtccacccag tccagcgccg aaagctgata gaaatgaacg
     6241 atatggtcat gcgtggtgtg cgcagccaga atgatgttac ggatgtattg cgcgttaacc
     6301 ggaacgtcga tattcagcgc actttctgcc gcacgaacgg aagacagcgc gtgagtggta
     6361 gtacatacgc cacagatacg ttgcacaatc atccatgcat cgcgcggatc gcggtttttc
     6421 acgatctctt ccatgccgcg ccacatggta ccggaagccc atgctttcga aacgacgcca
     6481 ttttcgattt cgcaatcgat gcgtaaatgc ccctcaatac gggttaccgg atcaatagta
     6541 attctctggc tcatgctttg ctcgcctcat gacgattatg atcgttttgt tttaaaggag
     6601 gaagtatcgg cagtagacga atgagtacga tgtaagcgca aatctcaata gccacaaaac
     6661 caatagaaat caacagttct tccctggtcg ggaagtaggc gtaaccgccg cccgggttga
     6721 atgccaccag cgaataggtc agacgccagg ttgcacaacc taacagtgcg ctcagtgctg
     6781 acaggaacag catgcgggaa tcattacgca gcttcgccac acgcagaacg accagcggga
     6841 agagcatcag caggacttca atccagaaca tcacggagta gaagtcaccg gcaaacgcta
     6901 acgacagctt gtcgcgatag ataagctcgc caaagcgcag cacgataaaa atcgccagca
     6961 acacactgat ggtgttggtc agcttgacga acagactctt ttcatccgga ccgttgccac
     7021 gcagacccgc ctgcaccagc gaaccttcaa agatgacaat cgagaagccc atgatgaacg
     7081 ccgtcagcag cgagaacagc ggcaacattt catagctctg ccacaacgga tgcaccttgt
     7141 agcccgccga gatcatcagt gaccccattg aagactggtg catggtcggc aacagcgcgc
     7201 caagtgcaat gatgaagaac atcaccttgt tcagacgctt gagagaaacc ttccagccca
     7261 gacgttcaaa cagtgccgga gcaaactcca gtgccatcac gccgatatag atggtcatac
     7321 agaccgccgt ctcgaacagt accgagttca cgttgaagtg acccggaatg tagaagtacg
     7381 gcaggttcca gtagcgaccc acgtcgatag tgatcgacaa gccacccagt gagtaaccaa
     7441 acagactcgc caacagcgcc ggacgcacca gcggatggta ttgcccacgg ttaaagacgt
     7501 ataccgccca cgccagcgcc cagccgccac aggcaaagcc ggtaccaatc agcaggtcaa
     7561 acgcgatcca cacgccccac gggaagccgc cgttcaggtc agagacagag cccagaccga
     7621 acaccagacg cttcacaatc aggagcatac agatgacgat taacggtcca aaaatcatga
     7681 ccggtttact gatgattttg ccgcccagcg gttgtggatc atgactcatg atcgtctcct
     7741 ccgtcgtgat ggtcgttttt ggtgttgcga cgaaccagca cggttaagcc cgccagcaca
     7801 gccagtggta gcatcatgcc tttatacagg gtgtgttgaa tattttcgga acgcgcaccg
     7861 gtagaaagat cgtccagttt cggcaggtcg agattttcat aaggcacacc cgtcagtacc
     7921 agaacctgag taccgccgcc ctctttctcg ccgtacagat gcggataata tttcggcacc
     7981 gtatgcaggt aagtgtcgcc agatttcagc gtctgacgcg gatagtggta ttcgctgcca
     8041 ggcttcagcg ccagacgttt tttcgcctcc gccatcagct cttcacgcgt accgaaaatc
     8101 accgcgcccg ccgggcacac ttctacgcag ccaggcagac cgcctttatc gagacgttcc
     8161 acacctttct ggttgcacag ctcgcactta tgcagcgcac caaacgggtt gttgtagtcg
     8221 tacttcggca cgttgtacgg acaggcgacc atgcagtaac ggcaaccggt gcacacgtct
     8281 ttgtcgtaat ggacaatgcc ggttttcgga tcttttttca gtgcagagac cgggcacaca
     8341 gagacacagt tcggatcgac gcagtgcata cactgtttct taatgtacgc gtagccgttc
     8401 tcctcctggt ctttgttgac ccctgtgccg ctggtccaca cctgaatgat gttattggta
     8461 tacggcgaca gtttgtcgtt gttcgaccag gtctgttccc cttgcgggtt acgttcaggg
     8521 aagttgatat cctgacactt ggtgacgcaa gcctggcagc ctacgcacaa ggtcgagtcg
     8581 tacaacatcc ccagcgatcc cggaattggc gggcggtttt cagcagccgc atgactgaca
     8641 gacggcagcg cgcccgtcag caatgccccg caggaggctg ctttaataaa attacgtctg
     8701 ttcacggtta ttctccccgt gagtcagcgt tatctttctt ttgctgacga cccagttcac
     8761 gcaccgccat cacgctgaca ccggcaacca gcccaaccac accgccgagc aaaccaatag
     8821 cgcctgcaga gacgttaccg ccctctttag cgttaacatc cggtttctgt gaacgcggag
     8881 tctgattttc gacgttggca agctgatgga tgcctttatg gaagccgata ccttcttcgt
     8941 tacagccata gcatgggtga ccaatcgcta ccggccacac gccgccaaca tcgcagaatt
     9001 gcagcgttga gcagttgccg taagtttctg gccctttaca gccgaggtgg tacaggcacc
     9061 agccttcacg gtggccttca tcaccgaact ctttggcaaa acgaccagca tcgaagtgcg
     9121 ggcgacgttc gcagtgttcg tgaatcagac ggccataggc gaaggtcgga cgatttttgt
     9181 catccagttt cggcggtttg ccgtaagtga tgatgtgcgc aacggtcgcg aggaagttgt
     9241 gcgggttcgg cgggcagccc ggaatattga taacggtttt gcctggcaga acttcttgca
     9301 ggctgactgc gccagttggg ttaactccag ctgcggcaac accgccccac gcagagcagg
     9361 aaccgatagc gataatcgct gctgcgcctt ctgccgcttt gcggatgtga tccacaatcg
     9421 gctcaccagc aaccatgcaa taaataccgt tatcttttaa tgggatggaa ccatccacaa
     9481 ccaacacata ctgccctttg tacttctcaa gtgcgttatg tttgttctct tcgacctgat
     9541 gaccgaaggc ggcggaaagc acttcgtgat actccagaga gatagtctcc agcacgaggt
     9601 tttctaccgt tggatgcgtt gcacgaagca gagattccgt acaaccggtg cactcctgcg
     9661 cgccaatcca gataactggc ggacgctgcg ggttagtaac cgattcggcc atctctgcag
     9721 cggctttgct acttaacccc atggtggcgg ctaatgctgc acaaagcttc atgaaatcac
     9781 gacggttaat gccgtgagaa tggatgaggg tgttatctcc agtcatttat agttattccg
     9841 ttgcgaagac ctggcatata ttttgcctca atcgcaaaat caataatgcg atcgatgcgc
     9901 catttaccac acatttatta tggttatcga tatcatgata ctgcggcggg gcgctaaaac
     9961 gaagggaaga agcattagtg taattaatta aagcaagata atacgtatgt ttgatcaatt
    10021 ttcgaggcga taaccagcca taaaaaagcg caacgtagaa caggaattat tctcacgaaa
    10081 gaaatccatt accatctctt tatccagtcc atagcggcgc gtcagaatac ccgcttccca
    10141 ggcgctaagc tggcgatctc cggtcttttc gtacatccgg tgtgagtagc ctaatacaaa
    10201 accgcgttta taatcggcac agaattttgt cacattaacc gcactgtcgg catgcgttgc
    10261 ttttaatccc gccattaagc ctttaccaaa atggttattc atcgttcacc ttattcgcta
    10321 tatattatca tatatagcaa tattttaggc aaagaacgac ttttgcgcac gctatttttt
    10381 catgcttgtt taagacgtaa tcccgtaaga gataaaccca tcaggagaga tatttatcga
    10441 accatttcag cgtcctttgc caggcaagat cggcggcaga tttgtcataa cgaggcgtgg
    10501 aatcattatg gaatccgtga ttaacccccg gatagatata cgcctcatag accttattat
    10561 tggctttcaa cgccgcctca taagcgggcc agccctcgtt gattcgggtg tccagttccg
    10621 cgaagtggag tagtaaaggc gcttcaatct tcgccacatc ggcagtgggt gcctgacgac
    10681 cataaaacgg caccgcgcag gccagttccg gatacgcgac agccgccgcg ttcgatacac
    10741 caccgccata gcaaaatccg gtaataccca cttatgcctg tcgcttgcgg atagcgttgc
    10801 ataaactcaa ttgcggcaaa gaaatcattc atcagtttgg ttggatcaac ctgctgttgc
    10861 agctcacgac ctttatcatc atttcccgga taacctc
//
LOCUS       DADLFA010000138        10856 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000138 DADLFA010000000
VERSION     DADLFA010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10856
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.139"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            83..424
                     /locus_tag="JQH65_003043"
     CDS             83..424
                     /locus_tag="JQH65_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flxA-like family protein"
                     /protein_id="HAY6140379.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLDVSLLNP
                     VEKTTHIDIYI"
     gene            complement(415..1341)
                     /locus_tag="JQH65_003044"
     CDS             complement(415..1341)
                     /locus_tag="JQH65_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY6140380.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            1431..2429
                     /locus_tag="JQH65_003045"
     CDS             1431..2429
                     /locus_tag="JQH65_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HAY6140381.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCTLPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(2426..2644)
                     /locus_tag="JQH65_003046"
     CDS             complement(2426..2644)
                     /locus_tag="JQH65_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6140382.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(2646..4661)
                     /gene="ligA"
                     /locus_tag="JQH65_003047"
     CDS             complement(2646..4661)
                     /gene="ligA"
                     /locus_tag="JQH65_003047"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAY6140383.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(4732..5718)
                     /gene="zipA"
                     /locus_tag="JQH65_003048"
     CDS             complement(4732..5718)
                     /gene="zipA"
                     /locus_tag="JQH65_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HAY6140384.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPVYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            5948..6709
                     /gene="cysZ"
                     /locus_tag="JQH65_003049"
     CDS             5948..6709
                     /gene="cysZ"
                     /locus_tag="JQH65_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HAY6140385.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            6894..7865
                     /gene="cysK"
                     /locus_tag="JQH65_003050"
     CDS             6894..7865
                     /gene="cysK"
                     /locus_tag="JQH65_003050"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAY6140386.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            8249..8506
                     /gene="ptsH"
                     /locus_tag="JQH65_003051"
     CDS             8249..8506
                     /gene="ptsH"
                     /locus_tag="JQH65_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HAY6140387.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            8551..10278
                     /gene="ptsI"
                     /locus_tag="JQH65_003052"
     CDS             8551..10278
                     /gene="ptsI"
                     /locus_tag="JQH65_003052"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HAY6140388.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            10319..10828
                     /gene="crr"
                     /locus_tag="JQH65_003053"
     CDS             10319..10828
                     /gene="crr"
                     /locus_tag="JQH65_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAY6140389.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLPGSVTVG
                     ETPVIRIKK"
BASE COUNT         2648 a         2803 c         2686 g         2719 t
ORIGIN      
        1 tgcgatttgt aagattgatt tttcttctca agttccttgt tcttctaccg atattacctc
       61 gtacagtgac gaggactgaa caatggccac tatctcttca acttcaattc cttccataca
      121 aacacagagt agtaacaggg catcacaagg tagcgatgtt gcttcacaaa tcgcgcggat
      181 aagtcagcaa atcatcaaac tcactcaaca aattaaagag attgtagaca ctagcggcag
      241 tgctgaagac aagcaaaaac aggccgaatt gattcaacag caaatcaccc tactggaaac
      301 acaactggcg cagttgcaga aacagcaggc agaaaaggcg caggagaaag agcagcgttt
      361 gtctctggat gtatcccttc taaatcctgt tgaaaaaacg actcatatcg atatctatat
      421 ctgatacaac ggatcccctt cccgaccagc cattagcgca ttgccctggt ctcgtaccac
      481 gtcccataac gcttcagcgg cggtggaaag tgaacggttc ttcctgcgca ccagcatcaa
      541 ctggcgttca acaaccggcg taatgcgctt taccaccagt gggctacctt caggcaatgg
      601 tagcgccagt gcgggaagaa tactgatgcc gatgcctgcg gctaccatcg ggaacagcgt
      661 cgccggatgt ccaatctcct gcacaatatt cgcctgaata ccattgcgcg ccagcgctgc
      721 gtcaatcagc ggtcggctgc ctgacgcgta atcctgcaac accaatttcg ctccctgtaa
      781 tgcctgccag ggtacataat cttccacggc caaagcgcta tcgcgatggc aaagcagaaa
      841 gaaaggctcg gaaagaatcg cttcacattg cagatcgccc actgggcctg gatcgatgac
      901 gatgccaaag tcgacatctc cctggcgaat actttccatc acccactgct gcggacgatc
      961 gtgcaggaca aactgaatat ctggatagcg gcgatggctt tcagcaatgc attgcggaat
     1021 aagatgcgcg gaaatggtct ggctggcagc gacccgaact tttccgctca gttgttgccc
     1081 catacgcccg gtatcgcgca acgtgctgtt aagttcatcc agcagtcgct caagacgcaa
     1141 agccaactgc tgccctgcat ctgtaagcac cacttcacgc gtggttctgt ccagcaggcg
     1201 aacaccagta tgattttcca gctccttcac actgtgactt actgccgact ggctcaagcc
     1261 gatacgctct cctgcacgac taaaactttt ctcctgcgct actgtgacga aaacctttaa
     1321 ttgttttaaa gaataattca tctgttttct tcatgaatgg atgcaataaa tcaattttat
     1381 ttctcaaact ggaagaagca caatagaacc atcgatcatc tggagtcttt atgaaacttt
     1441 ttcgtatcct cgatcctttc accttaaccc tgatcacggt ggtgttgctg gcctctttct
     1501 ttccggccag aggcgatttc gtccccttct ttgaaaatct gaccaccgca gctattgccc
     1561 tgctgttctt tatgcacggc gcgaagttgt cgcgtgaggc gattattgct ggcggtggtc
     1621 actggcgact gcatttgtgg gtaatgtgca gcaccttcgt gctgtttccg attctgggtg
     1681 tactgtttgc ctggtggaaa ccggtaaatg tcgacccgat gctctactcc ggttttctct
     1741 acttgtgcac tctcccggct accgtgcagt ctgcaatcgc cttcacgtca atggcgggcg
     1801 gtaacgtcgc ggcggcggtt tgttctgcgt cagcatccag cctgctggga attttccttt
     1861 caccattgct ggttggtctg gtgatgaatg ttcacggtgc agggggcagc cttgagcagg
     1921 tcggtaaaat tatgctgcaa ctgctgctgc cgtttgtgtt ggggcatctt tcccggccgt
     1981 ggattggtga ctgggtgtcg cgcaataaaa aatggattgc gaaaactgac cagacgtcca
     2041 ttctgttggt ggtttataca gcgttcagcg aagccgtcgt taatggtatc tggcataaag
     2101 ttggctgggg atcattgctg tttatcgtgg tggtcagctg cgttcttctg gctatcgtga
     2161 ttgtagttaa cgtctttatg gcacgccgac tgggcttcaa taaggcagat gaaattacta
     2221 tcgtcttttg tggttcgaaa aagagtctgg caaatggcat cccgatggca aacattctgt
     2281 tccccacatc ggtgatcggt atgatggtgc tgcccctgat gattttccat cagatccaat
     2341 tgatggtctg tgcggtgctg gcgcgtcgat acaaacgcca gaccgaacag ttacaggcgc
     2401 agcaggaaag cagcgccgat aaagcttaaa gcggacgctt caggggctgg accagttgcg
     2461 tcagcccctc ggttttaatc agcagcgtga tttgcattag ctcaccgagc ttccctgccg
     2521 ggaactcatc tttgcgggca aaccacagca gatactcctc cggcaggtcg attaagcgac
     2581 gccctttgta tttgccaaac ggcattatcg tattggctat ttcaatcagc tgctcttttt
     2641 ccatctcagc tacccagcaa acgcagcatt tccgcttcgt cgatgacttc aatgcccagt
     2701 tcctgcgcct tcgccagttt agatcctgca gcttcacccg caatcaccag atcggttttc
     2761 ttcgacacgc tgcccgcgac tttcgcgccc agttcgacca gtcgagcttt agcgtcatca
     2821 cgcgacatct ggcttaagct gcccgtaagc accacggttt taccagcaaa cgggctgtca
     2881 atctcttccg cgttgataac gatcggcgca ggccagtgaa caccttccgc caacagctcg
     2941 ctgatgacat tgcggttgct ttcttcggca aagaagttgt gaacgtggga tgcaacgaca
     3001 atgccaacat caggcacctt ttgcagctct tcaatcgaag cggcttccag cgcttccagc
     3061 gtgccgaaat atgccgccag acctgctgcg gtggcctcgc cgacttcacg gatgccaagt
     3121 gcatagagga agcgagcaaa ggtggtttct ttcgcttttt ccagcgcgtt aaccacgttt
     3181 tgtgccgatt ttggccccat acgctccagt ccggtcagtt tgcctgcggt gagtttgaac
     3241 agatctgccg gagtgtggac atattctttt tcaaccagct gatcgatgat tttgtcgccc
     3301 attccgtcaa catccatcgc acggcgggaa acaaagtgtt tcagcgactc tttacgctgc
     3361 gcaccgcaaa tcaggccacc ggtacagcgg gcaaccgctt caccttccac acgctcaacg
     3421 tcagaaccac ataccggaca atgcgtcggg aatacaacct cacgggtatc ttccgggcgt
     3481 tcagaaagca cgacgttaac cacctgcggg atcacgtcgc cagcgcggcg aatcaccact
     3541 ttatcgccaa tgcgtaaacc aagacgttcg atttcatccg cattgtgtaa ggttgcgtta
     3601 ctcaccagca cgccagcaac atggacaggt tccagacgcg caacaggcgt aatcgcgcca
     3661 gtacgcccaa cctgaaactc gacgtcacgc acaaaggtca tctgctcctg cgccgggaat
     3721 ttaaacgcta ccgcccagcg cggggcacgc gcgacaaagc caagctgctc ctgctgttcc
     3781 agtgagttga ccttaatcac cacgccgtcg atatcaaagc ccagcgtcgg gcggtcttct
     3841 tccactttgt gatagaacgc cagcacttct tccgccgatt cacaaagcgt tacccgatcg
     3901 ctgaccggca acccccactt tttaaattgc agtaaacggc caagatgagt atccggcagc
     3961 tcgccacctt ccagaacacc aacgccatag cagaaaaaag tgagcggtcg cttcgctgta
     4021 atacgcggat caagctgacg cagtgaacca gctgccgcat tacgtgggtt agcaaaaact
     4081 ttcccgcccg tgcgtcgcgc atcttcgtta atcttttcga accccgcctg cggcaggaac
     4141 acttcaccac gcacttccag acgcgccggg atattctctc catgcagctt cagcggaatg
     4201 gcgcgaatag tacgcacatt agacgtgata tcttccccgg tggtgccatc gccacgggtc
     4261 gcggcactga ctaaaacgcc attttcatac agaatactga cggcaaggcc atccagcttc
     4321 agctcacagc accaggtgac tttctcgttg tttttcagac ggtcctgcac acgtttgttg
     4381 aaagcaagaa agctttcttc atcaaaaacg ttatccagtg acagcattgg tacttcatgg
     4441 cgtatctggc tgaaagccgc cagcggcgca gcacctacac gttgggtagg cgaatcaggc
     4501 gtaatcagtt ctggatgttt agtttccagc tcgcgcagtt cgcgcatcag cctgtcgtat
     4561 tcagcgtcgg gaatttccgg cgcatccatc acatgataaa gatattcatg atggcgaagc
     4621 gtcgttcgca gttctgtcag ttgttgttcg attgattcca tatcgcacca tcaatgctaa
     4681 aaacccccga caagcggggg ttcgaagaag agttaatttg ccttaagtgt atcaggcgtt
     4741 ggcgtctttg acttcgcgga tgatgtcctg gtactcgcgc aatttctgcg gagtcatcat
     4801 acggcgctga tcgtcaagca cgacaccgcc cacttcatcg gcaatatgct gcgcagattg
     4861 cagcatcagc ttgaagttct gcagctcgtc accgtaagac ggtacctgca taaagatagt
     4921 gacacccgga gtagtgaaat ccttcatttc aggatcaaag gttcccggtt tcaccatatt
     4981 cgccaggctg aataacgccg ggccgctgcc atccgggcta agatgacgat ggtaaatatt
     5041 catatcgcca aaaatgaagc ccgcttgttg aatgctgtta agaagcagtt caccgtttag
     5101 ctcgctaccg tgatgcgccg cgacgttcat gataatcacc gcttctttgc gcttcggttt
     5161 atccataact ggagccggtt ccgctacagg ctcaggctgt ggtgccgcta cgggttctgc
     5221 aggctggaaa gcctgttgtg ccggttgcgg tgctgaatgc acaggctgcg gcgctggcgc
     5281 gacctgtggc gaaactggct gctgcaacgg ctgttcaggc tgcggctgat agacaggctg
     5341 ctgcaccggc tgcgctggac gcggagcatg ttgcggcggt acctgcgctt caggcggctg
     5401 ctggaccggt tgacgcggct gcgcagacgc ataaggcggt tggtactggt gttgcggcga
     5461 cggacgagca gcctcatgct cctgagcgtt agccggggca tgattcacgc ggtgaacacg
     5521 aacctcacca acgccctcat catcttcgac atcctcgtca taagaatcgt cgtcacgttt
     5581 tgacttcatt cgttttaatg gccgatcgcg gaacatagaa gatcgttctt tacggctggt
     5641 ccagaaacca tgtaccagta aagcgattat ggcgatcgcg ccaacaatga ttaatatcag
     5701 acgcaaatcc tgcatcatta tattctctgt tgttctaaca ccttgccacc acggcaaaca
     5761 tttactcact aagagtattt gccgattacc tcaagtgcaa gtgcactatt aactttcaca
     5821 gcacaaagat agatgaaatc gtgctttttg ctgttttttc gaacatatcc taactgtcca
     5881 ttgcgcaatt acccggtaaa atacgcagaa ttttcctagg attggtcaaa aggagctcat
     5941 cctgattatg gtttcatcat tcacatctgc cccacgcagc ggtttttact attttgcgca
     6001 aggctggaag ctcgtctcgc aacctgggat tcggcgtttc gttattttac cgctgctggt
     6061 caatattttg ttgatggggg gcgcattctg gtggctcttt acacagctcg atgtctggat
     6121 cccgactctc atgagttacg ttccggactg gctacaatgg ctgagttatc tgttgtggcc
     6181 tctggctgtc atctctgtgc tgttagtgtt tggctatttc ttctccacga ttgctaactg
     6241 gattgccgct ccgtttaacg gtttattggc tgaacaactg gaagcacgat tgactggcgc
     6301 tacaccgcca gataccggga ttttcggtat catgaaagat gtgccgcgaa tcatgaaacg
     6361 cgaatggcaa aaatttgcct ggtatctgcc gcgcgcaatt gtattactaa ttctttactt
     6421 catccctggt attgggcaaa ccgtcgcgcc ggtactgtgg ttcctgttta gcgcctggat
     6481 gttagccatc cagtattgcg attacccctt cgataaccac aaagtgccgt ttaaagagat
     6541 gcgcaccgcc ctgcgcacac gcaaaatcac caatatgcag tttggtgctt taaccagcct
     6601 gtttacgatg atcccgctgc ttaatctgtt catcatgccc gttgccgttt gtggcgcgac
     6661 ggcgatgtgg gtcgattgct atcgcgataa acacgcgatg tggcggtaac aatctaccgg
     6721 ttattttgta aaccgtttgt gtgaaacagg ggtggcttat gccgcccctt attccatctt
     6781 gcatgtcatt atttcccttc tgtatataga tatgctaaat ccttacttcc gcatattctc
     6841 tgagcgggta tgctacctgt tgtatcccaa tttcatacag ttaaggacag gccatgagta
     6901 agatttttga agataactcg ctgactatcg gtcacacgcc gctggttcgc ctgaatcgca
     6961 tcggtaacgg acgcattctg gcgaaggtgg aatctcgtaa ccccagcttc agcgttaagt
     7021 gccgtatcgg tgccaacatg atttgggatg ccgaaaagcg cggcgtgctg aaaccaggcg
     7081 ttgaactggt tgaaccgacc agcggtaata ccgggattgc actggcctat gtagctgccg
     7141 ctcgcggtta caaactcacc ctgaccatgc cagaaaccat gagtattgaa cgccgcaagc
     7201 tgctgaaagc gttaggtgca aacctggtgc tgacggaagg tgctaaaggc atgaaaggcg
     7261 caatccaaaa agcagaagaa attgtcgcca gcaatccaga gaaatacctg ctgctgcaac
     7321 aattcagcaa tccggcaaac cctgaaattc acgaaaaaac caccggtccg gagatctggg
     7381 aagataccga cggtcaggtt gatgtattta ttgctggcgt tgggactggc ggtacgctga
     7441 ctggcgtcag ccgctacatt aaaggcacca aaggcaagac cgatcttatc tctgtcgccg
     7501 ttgagccaac cgattctcca gttatcgccc aggcgctggc aggtgaagag attaaacctg
     7561 gcccgcataa aattcagggt attggcgctg gttttatccc ggctaacctc gatctcaagc
     7621 tggtcgataa agtcattggc atcaccaatg aagaagccat ttctaccgcc cgtcgtctaa
     7681 tggaagaaga aggtattctt gcaggtatct cttctggagc agctgttgcc gcggcgttga
     7741 aactacaaga agatgaaagc tttaccaaca agaatattgt ggttattcta ccatcatcgg
     7801 gtgagcgtta tttaagcacc gcattgtttg ccgatctctt cactgagaaa gaattgcaac
     7861 agtaatgcca gcttgttaaa aatgcgtaaa aaagcacctt tttaggtgct tttttgtggc
     7921 ctgcttcaaa ctttcgcccc tcctggcatt gattcagcct gtcggaactg gtatttaacc
     7981 agactaatta ttttgatgcg cgaaattaat cgttacagga aaagccaaag ctgaatcgat
     8041 tttatgattt ggttcaattc ttcctttagc ggcataatgt ttaatgacgt acgaaacgtc
     8101 agcggtcaac acccgccagc aatggactgt attgcgctct tcgtgcgtcg cgtctattaa
     8161 aaactggcgc taacaataca ggctaaagtc gaaccgccag gctagacttt agttccacaa
     8221 cactaaacct ataagttggg gaaatacaat gttccagcaa gaagttacca ttacagctcc
     8281 gaacggtctg cacacccgcc ctgctgccca gtttgtaaaa gaagctaagg gcttcacttc
     8341 tgaaattact gtgacttcca acggcaaaag cgccagcgcg aaaagcctgt ttaaactgca
     8401 gactctgggc ctgactcaag gtaccgttgt gactatctcc gcagaaggcg aagacgagca
     8461 aaaagcggtt gaacatctgg ttaaactgat ggcggaactc gagtaatttc ccgggttctt
     8521 ttaaaaatca gtcacaagta aggtagggtt atgatttcag gcattttagc atccccgggt
     8581 atcgctttcg gtaaagctct gcttctgaaa gaagacgaaa ttgtcattga ccggaaaaaa
     8641 atttctgccg accaggttga tcaggaagtt gaacgttttc tgagcggtcg tgccaaggca
     8701 tcagcccagc tggaaacgat caaaacgaaa gctggtgaaa cgttcggtga agaaaaagaa
     8761 gccatctttg aagggcatat tatgctgctc gaagatgagg agctggagca ggaaatcata
     8821 gccctgatta aagataaaca catgacagct gacgcagctg ctcatgaagt tatcgaaggt
     8881 caggcttctg ccctggaaga gctggatgat gaatacctga aagaacgtgc ggctgacgta
     8941 cgtgatatcg gtaagcgcct gctgcgcaac atcctgggcc tgaagattat cgacctgagc
     9001 gccattcagg atgaagtcat tctggttgcc gctgacctga cgccgtccga aaccgcacag
     9061 ctgaacctga agaaggttct gggtttcatc accgacgcgg gtggccgtac ttcccacacc
     9121 tctatcatgg cgcgttctct ggaactgcct gctatcgtgg gtaccggtag cgtcacctct
     9181 caggtgaaaa atgacgacta tctgattctg gatgccgtaa ataatcaggt ttacgtcaat
     9241 ccaaccaacg aagttattga taaaatgcgt gctgttcagg agcaagtggc ttctgaaaaa
     9301 gcagagcttg ctaaactgaa agatctgcca gctattacgc tggacggtca ccaggtagaa
     9361 gtatgcgcta acattggtac ggttcgtgac gttgaaggtg cagagcgtaa cggcgctgaa
     9421 ggcgttggtc tgtatcgtac tgagttccta ttcatggacc gcgacgcgct gcccactgaa
     9481 gaagaacagt ttgctgctta caaagcagtg gctgaagcgt gtggctcgca ggcggttatc
     9541 gttcgtacca tggacatcgg cggcgacaaa gagctgccat acatgaactt cccgaaagaa
     9601 gagaacccgt tcctcggctg gcgcgctatc cgtatcgcga tggatcgtaa agagatcctg
     9661 cgcgatcaac tccgcgctat cctgcgtgcc tcggctttcg gtaaattgcg cattatgttc
     9721 ccgatgatca tctctgttga agaagtgcgt gcactgcgca aagagatcga aatctacaaa
     9781 caggaactgc gcgacgaagg taaagcgttt gacgagtcaa ttgaaatcgg cgtaatggtg
     9841 gaaacaccgg ctgccgcaac aattgcacgt catttagcca aagaagttga tttctttagt
     9901 atcggcacca atgatttaac gcagtacact ctggcagttg accgtggtaa tgatatgatt
     9961 tcacaccttt accagccaat gtcaccgtcc gtgctgaact tgatcaagca agttattgat
    10021 gcttctcatg ctgaaggcaa atggactggc atgtgtggtg agcttgctgg cgatgaacgt
    10081 gctacacttc tgttgctggg gatgggtctg gacgaattct ctatgagcgc catttctatc
    10141 ccgcgcatta agaagattat ccgtaacacg aacttcgaag atgcgaaggt gttagcagag
    10201 caggctcttg ctcaaccgac aacggacgag ttaatgacgc tggttaacaa gttcattgaa
    10261 gaaaaaacaa tctgctaatc cacgagatgc ggcccaattt actgcttagg agaagatcat
    10321 gggtttgttc gataaactga aatctctggt ttccgacgac aagaaggata ccggaactat
    10381 tgagatcatt gctccgctct ctggcgagat cgtcaatatc gaagacgtgc cggatgtcgt
    10441 ttttgcggaa aaaatcgttg gtgatggtat tgctatcaaa ccaacgggta acaaaatggt
    10501 cgcgcctgta gacggtacca ttggtaaaat ctttgaaacc aatcacgcgt tctctatcga
    10561 atctgatagc ggcgttgaac tgttcgtcca cttcggtatc gacaccgttg aactgaaagg
    10621 cgaaggcttc aagcgtattg cagaagaagg tcagcgcgtg aaagttggcg atactgtcat
    10681 tgaatttgat ctgccgctgc tggaagagaa agccaagtct accctgactc cggttgttat
    10741 ctccaacatg gacgaaatca aagaactgat caaactgccc ggtagcgtaa ccgtgggtga
    10801 aaccccggtt atccgcatca agaagtaatt cttgccgcag tgaaaaatgg cgccca
//
LOCUS       DADLFA010000139        10697 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000139 DADLFA010000000
VERSION     DADLFA010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10697
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.140"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(88..237)
                     /gene="hokB"
                     /locus_tag="JQH65_003054"
     CDS             complement(88..237)
                     /gene="hokB"
                     /locus_tag="JQH65_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HAY6140390.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            636..2249
                     /gene="trg"
                     /locus_tag="JQH65_003055"
                     /pseudo
     CDS             636..2249
                     /gene="trg"
                     /locus_tag="JQH65_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
     gene            complement(2287..3209)
                     /locus_tag="JQH65_003056"
                     /pseudo
     CDS             complement(2287..3209)
                     /locus_tag="JQH65_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            3426..4769
                     /locus_tag="JQH65_003057"
     CDS             3426..4769
                     /locus_tag="JQH65_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAY6140391.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GGPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEYRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHLGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            4994..6649
                     /locus_tag="JQH65_003058"
     CDS             4994..6649
                     /locus_tag="JQH65_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HAY6140392.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6789..7013
                     /locus_tag="JQH65_003059"
     CDS             6789..7013
                     /locus_tag="JQH65_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HAY6140393.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGFDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKGV"
     gene            7076..7612
                     /gene="rimL"
                     /locus_tag="JQH65_003060"
     CDS             7076..7612
                     /gene="rimL"
                     /locus_tag="JQH65_003060"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HAY6140394.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7607..8587)
                     /locus_tag="JQH65_003061"
                     /pseudo
     CDS             complement(7607..8587)
                     /locus_tag="JQH65_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
     gene            8711..9703
                     /gene="tehA"
                     /locus_tag="JQH65_003062"
     CDS             8711..9703
                     /gene="tehA"
                     /locus_tag="JQH65_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HAY6140395.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATMPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9700..10293
                     /gene="tehB"
                     /locus_tag="JQH65_003063"
     CDS             9700..10293
                     /gene="tehB"
                     /locus_tag="JQH65_003063"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HAY6140396.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKLGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            10596..>10697
                     /locus_tag="JQH65_003064"
     CDS             10596..>10697
                     /locus_tag="JQH65_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HAY6140397.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGF"
BASE COUNT         2736 a         2497 c         2712 g         2752 t
ORIGIN      
        1 aacatgcact aactgaagtg taaaaggggt gccatgagaa aatgaatctg ctagtcagat
       61 gcgcggggaa aatccccgcg cttgccctta cctggacgtg caggccatga gcgcagcaac
      121 ctccttatca ccgtcccgga accgcagttc gtagagcgtt tgtcgggtca ggagtgtgaa
      181 tgtcagaatc gtaatgcaga taatgagcag acacaccacc agagggttgt gcttcatagc
      241 ctttctcctt gccggatggc gggtaagagg ctaagatctg aattgctagg ttcattcgtt
      301 ggcctcggtt gatagaaata tcggtcgggg ccttcgtctt tctgattccc ggttagcctg
      361 aaaacagaaa gtctcaggca cccgcaggca tcctatgagg tttccttagg gacgaaaata
      421 atcacttcac gaaattgcgt gctgttttcc agaatttttc gtcattcgga ttagccagtt
      481 tagccattcg ttactctctt cattccaata gcattaattt tctctgcaat aattgttgta
      541 aaaatgtgac gcaaagaggt ttttggtcat aagtaattac cgtcaagtgc cgatgacttt
      601 ctatcaggag taaacctgga cgagagacaa cggtaatgaa tacaactccc tcacagcgat
      661 taggtttttt gcatcacatc aggctggttc cgttatttgc ctgcattctt ggcggtatct
      721 tagttctatt cgcattaagt tctgctctgg caggccgatc gcgatcagcg tgatgttact
      781 gcggagattg agattcggac cgggttagcg aacagttcag attttttgcg ttcagcccgg
      841 atcaatatga ttcaggccgg ggctgcgagt cgtattgcgg aaatggaagc aatgaagcga
      901 aatattgcgc aagccgaatc ggagattaaa cagtcgcagc aaggttatcg tgcttatcag
      961 aatcgaccgg tgaaaacacc tgctgatgaa gccctcgaca ctgaattaaa tcaacgcttt
     1021 caggcttata tcacgggtat gcaacctatg ttgaaatatg ccaaaaatgg catgtttgaa
     1081 gcgattatca atcatgaaag tgagcagatc cgaccgctgg ataatgctta taccgatatt
     1141 ttgaacaaag ccgttaagat acgtagcacc agagccaacc aactggcgga actggcccat
     1201 cagcgcaccc gcctgggtgg gatgttcatg attggcgcgt ttgtgcgtgc cctggtcatg
     1261 acgctgataa catttatggt gctacgtcgg atcgtcattc gtccactgca acatgccgca
     1321 caacggattg aaaaaatcgc cagtggcgat ctgacgatga atgatgaatc ggcgggtcgt
     1381 aatgaaatcg gtcgcttaag tcgtcattta cagcaaatgc agcattcact ggggatgaca
     1441 gtagggactg ttcgacaggg cgcggaagag atttatcgtg gcaccagcga aatttcagct
     1501 ggcaatgcgg acctgtcatc tcgcaccgaa gaacaagcgg cggctatcga acaaactgcc
     1561 gccagcatgg agcaactcac tgcgacggtg aaacagaatg cggataacgc gcatcatgcc
     1621 agcaaactgg cgcaagaggc ttctattaaa gccagcgatg gcgggcagac ggagtaaaaa
     1681 cgatgggcgc tatctccacg agttcgaaga aaatttctga gatcaccgcc gtcatcaaca
     1741 gtattgcttt ccagacgaat attctggcac tgaatgctgc cgttgaagcc gcgcgagcgg
     1801 gtgagcaagg gcgtggattt gccgttgtcg ccagcgaagt acggacactc gcaagtcgca
     1861 gcgctcaggc ggcgaaagag attgaaggct tgatcagtga atcagtcagg ttaattgacc
     1921 tggggtcgga tgaggtggca acggccggga aaaccatgag cactattgtt gatgccgtcg
     1981 cgagtgtcac acatatcatg caggaaatcg ccgccgcctc ggatgaacaa agtagaggca
     2041 taacgcaggt tagccaggcg atttctgaaa tggataaggt gacgcaacag aatgcttctc
     2101 tggtagaaga ggcctcagcg gcggcggtgt cccttgaaga acaggcggca cgattaactg
     2161 aggcggtgga tgtattccgt ctgaacaaac attctgtgtc ggcagaacct cgcggagcgg
     2221 gtgaaccagt tagtttcgct acggtgtgaa aatgttcaag gagggatcga cagatccctt
     2281 cacctttcag aacggcattg attttcgaat agcgttaatc atcaactggc aaccagaaga
     2341 gaacatcgca tctacgcggg tcagtattcc aatcggttcg cctgcaccat gtcccggaac
     2401 aggctgggcc accagcgtgg catgacgcag gtcgtctttt accgcgccag aagggacaaa
     2461 ccacacgtag tcgtattcaa ccgtaagttg acgagatagc gaagcagaca gcgtttcgat
     2521 acaacccgaa ggaattttac atccctggct ctgtactaat gcatctgaat gctggcgtgg
     2581 cgcagtgcct tctggtgata caacgaccgg ccatttcagc actcggctta gcgttacgtt
     2641 ctcctgaagt agcgggtgat tagggcggac aaccagcttc agcgattcaa gaaacagcag
     2701 ttcgtaatta agcccggtca tcagttcagg atctgacatc cgaccaatgc cgatatcgat
     2761 ttccccggtt ttcaaacccg ccagaatcat agggttactc attgtcgcaa cttgcaaggt
     2821 cgtctctttt tgttgctgat gaaactgacc tataaccgaa ggtaatatcc ccagtgccgc
     2881 agtaggtagt gcaccaatcc tgacgacatc attattaaga ccttctttac gatgaagcga
     2941 ctgtccggca gtgttgatgg cgtcaagaac tctgactgca tgcgttaaaa attgttcgcc
     3001 gggtaaggta agttgcgccc cctgacgacc acgctcaaac aagcgagcgc ccgtcagctg
     3061 ctacagttca ttcaatgtct tagagagcgc aggttgactc aaattaaggg tttcagccgc
     3121 gcccccaaag ttccttgttg tgcgacagct acgaatgtat gaaggtggcg caaacggatg
     3181 cgctgactaa acagactatt tttttccata agcgatgtta aaaacgaagc ggtgtcgctg
     3241 acaagtgaag ttgtttgatt atgataactt gattgcaaaa tataattaac aattaaagca
     3301 attgtgttac agcaaaatgg ataatattga tgttttcgcg gcgagatcac agtttgtaaa
     3361 ttcttcccgc cagagtgaat gcggttacct acactccaga ttactgacca ctggaggcag
     3421 acactatggc gaacagcatc acggcggatg agattcggga acagttttcg caggcaatgt
     3481 cagccatgta ccagcaagaa gttccgcagt acggcacgct gctggaactg gtagctgatg
     3541 tgaatctggc tgtgctggaa aacaatcctc aactgcacga aaaaatggta aatgcagatg
     3601 agctggcgcg actgaatgtt gaacgtcatg gggcgattcg cgttgggact gcacaagagc
     3661 ttgctactct tcggcggatg tttgccatta tggggatgta cccggtgagc tattacgatc
     3721 tctcgcaggc aggggggccg gtacattcga cagcatttcg gcccattgat gatgcttctc
     3781 tggcgcgtaa tcccttccgc gtttttacct ccttactccg ccttgagctt atcgagaacg
     3841 aaattttgcg ccagaaagcg gcggagattc tgcgtcagcg cgatatcttc accccacgtt
     3901 gtcgacaact gttagaggaa tatgagcagc agggcggttt taacgaaaca caggcacagg
     3961 aatttgtgca agaggccctg gaaacgtttc gctggcacca gtcagcaacg gtagatgaag
     4021 aaacctatcg cgccttgcac aacgaatatc ggttgattgc tgatgtggtc tgttttcctg
     4081 gatgccatat caaccacctg acgccacgta cgctggatat tgaccgggtg cagtcgatga
     4141 tgcctgaatg cggaattgaa cccaaaattc tcatcgaggg gccgccgcgc cgcgaggtac
     4201 cgattttact acgccagacc agctttaaag cactggaaga gacggtgttg tttgcggggc
     4261 agaaacaggg cacgcatacc gcgcgctttg gtgaaattga gcagcgtggc gtggcattaa
     4321 cgccgaaagg gcgacaactg tatgatgatc ttctgcgtaa cgctggaacc gggcaggata
     4381 atctcactca ccaaatgcat ttacaggaaa ccttccgcac ttttcctgac agtgagtttt
     4441 taatgcgtca gcaagggttg gcatggttcc ggtaccgtct gacgccttcg ggtgaggcgc
     4501 atcgtcaggc gattcatctt ggagacgatc cacagccttt aattgaacgt ggttgggtag
     4561 tggcgcaacc catcacctat gaagatttct tgcccgttag cgcggcgggg atcttccagt
     4621 caaatctggg taatgaaacg cagacacgca gtcacggtaa tgccagtcgc gaagcatttg
     4681 agcaggcgtt gggttgtccg gttttggatg agttccagct ttaccaggaa gcggaagaac
     4741 gcagtaaacg tcgctgtggt ttgctttaaa ttctgaccat ccgcctttgc aaaaatttgc
     4801 ctgattttac aaacgaatca ggctcatccc atcgacataa aaaaaatgcc gatttatgca
     4861 tattctctca gttcaacaat tggattatta ataaatattg tctagagtga gcggtcataa
     4921 ataagcactt tcttgccgct gaaaacgacc agcgcgggac cattcacaac accagaagga
     4981 ctcactttca ggtatggatc gtagacgatt tattaaaggt tcaatggcta tggccgccgt
     5041 gtgcggtacc agcggcattg cttctctttt ttctcaggcg gcattcgcgg cagattctga
     5101 tattgccgac gggcaaaccc agcgttttga cttctccatt ctacagtcaa tggcgcacga
     5161 cttagcgcaa acagcgtggc gtggtgcgcc tcgtccgtta cctgacacgc tggcgacaat
     5221 gacgccgcag gcttataaca gtattcaata cgacgccgaa aaatcgctct ggcataacgt
     5281 tgagaaccgt caactggacg ctcagttctt ccatatggga atgggattcc gtcgccgcgt
     5341 tcgtatgttt tctgtagatc cagcaacaca tctggcgcgt gaaattcact ttcgcccgga
     5401 gttgttcaaa tacaacgatg cgggtgttga tacaaaacaa ttagaagggc aaagcgatct
     5461 cggctttgcc ggttttcgcg tgtttaaagc ccccgaactg gcgcgccgtg atgtagtatc
     5521 atttctcggc gcgagttatt tccgcgccgt tgatgataca tatcaatacg gtttgtcggc
     5581 ccgcggcctg gcgatcgaca cttacaccga cagtaaagaa gagttccccg actttaccgc
     5641 cttctggttt gatacggtaa aaccgggggc aactaccttt accgtttatg cgttgctcga
     5701 tagcgccagc attactggtg cctataagtt cactatccat tgtgagaaaa gtcaggtgat
     5761 tatggatgtg gaaaatcacc tgtatgcgcg caaagacatt aaacagctgg gcattgcgcc
     5821 gatgaccagt atgttcagct gcggtactaa tgaacgtcgg atgtgcgata caattcatcc
     5881 gcaaattcat gactctgatc gtctgtccat gtggcggggc aacggcgagt ggatttgccg
     5941 tccgctgaat aatccgcaaa aattgcagtt caatgcttac accgacaaca acccgaaagg
     6001 gtttggttta ttgcaactgg atcgtgactt ctcccattat caggacatta tgggctggta
     6061 taacaaacgc ccaagtctgt gggtggaacc gcgtaacaag tggggtaagg gcaccatcgg
     6121 cctgatggaa atcccaacaa cgggcgaaac gctggataac attgtctgtt tctggcagcc
     6181 agaaaaagct gtaaaagcag gtgatgagtt tgcattccag tatcgtctgt actggagtgc
     6241 gcaaccgcct gttcattgcc cattagcgcg cgttatggcg acgcgtaccg gcatgggcgg
     6301 tttcccggaa ggttgggcgc caggtgaaca ctatcccgaa aaatgggcgc gtcgttttgc
     6361 cgtcgatttc gttggtggtg atctgaaagc tgccgcgcca aaaggcattg agccggtgat
     6421 tacgctttcc agtggggaag cgaagcaaat cgaaattctc tatattgaac ccatcgatgg
     6481 ttatcgtatt cagtttgact ggtatccgac ttcggactcc actgatccgg tcgatatgcg
     6541 gatgtatcta cgttgtcagg gggacgctat cagtgaaaca tggctgtatc agtatttccc
     6601 gccagcgccg gataaacgtc agtatgttga cgaccgcgtg atgagttaat cgttttttct
     6661 tcggcacctt cttcgggagg tgccgtctgg ttaaacacga tcccgctcgc atttttccct
     6721 aagttaaatg agtaatctga tggtgtgtat ttcagataca ccttgtcagc cactaacagg
     6781 gagtgcgtat gtttccagaa taccgagatt taatatcccg tctgaaaaac gaaaatcctc
     6841 gctttatgtc cttgttcgat aaacacaata aacttgatca tgaaattgcc agaaaggaag
     6901 gtttcgacgg tcgagggtac aatgcggaag tggtccgcat gaaaaaacaa aagctacagt
     6961 taaaagatga gatgctcaaa atcctgcagc aggagagcgt caaaggggtg taaactttcc
     7021 taagccgcca gccaggcggc ttttttaaca acagcatgga ttgactggag ataagatgac
     7081 tgaaacgata aaagtaagcg aatcacttga attacatgct gttgcggaaa gtcacgtcac
     7141 acctctttat cagttaatct gtaaaaataa aacctggtta cagcagtcgc taaactggcc
     7201 gcagttcgtt cagagtgaag aggacacgcg aaaaacggtg cagggtaatg tgatgttgca
     7261 tcaacgcggc tatgccaaaa tgttcatgat tttcaaagaa gatgaactta tcggcgttat
     7321 ctcgtttaat cgtattgaac cactgaataa aactgctgaa atcggctact ggctggacga
     7381 atctcatcag gggcagggga tcatttctca ggcgctgcag gcattgattc atcattacgc
     7441 tcagtctggt gaacttagac gcttcgtgat caaatgtcgg gtggacaatc cgcaaagcaa
     7501 ccaggtcgct ttgcgcaatg gttttatcct tgaaggttgc ctgaaacagg ctgagttcct
     7561 gaatgatgcc tatgatgatg tgaacttata cgcgcgtatt atcgattcat aataaccctg
     7621 ccagcggcgt tcgcgtaatg cgctcctcgc cgttaatgac ttttggacca cggacatgaa
     7681 ccgtgtcgcc atcaaaggct tcaacgacgg cgtggtcggt taactccaca tggttttcaa
     7741 ttatcaccgc acctgaaata cggctctcac cttgtattac aacgtgctcg tcgagcagaa
     7801 tcggttcacc acgtaccacg gcatttccgc caatcaagac atgatgtttt aacacacaat
     7861 taccttccac aatggcatat tccgccacct gcgaactgta atgaatcgtc ggaatggcat
     7921 cttcttctat gccagctttc acctgcgcgt gaccgtagac tttagcgcaa tcgcatagcc
     7981 agacgttgtt ctcttcatta ccttcaacac tggcaaaatc aaaaacttcg gcgcgatgtt
     8041 caataaaagc ataccggacg acggcatcgc cataaatttg tgcctggtgg acaatacgcg
     8101 aggcgctaac ccttgcgcga tcataaattt ggagcaggag ttgatggtcg ggcgttaagc
     8161 cttgtgcggc gacgatcata gaatgttgat caattaaggc gtgaccaaat attctacatt
     8221 gcccgtaaac taatgagtca tgaatggtga cactgtcact tatatatgca ccctgactga
     8281 tttcactatt atcgatccag acgttatccg ttgcgtaaac ctctccccac agcacgctgg
     8341 tgccggtgat acgcgtatta ccagaaatca ccgttccgcc gaacgcaata gcattctggt
     8401 cgtaaatcca gcaatttcct tcctatgcta acaccgtttc gcgatcgatc cagccgccag
     8461 cggttcctgc tatcacatcg ttaaaatcgc taatggcgat aatctgccgt aataacacac
     8521 tttttttagt gccatcttcc tgataactaa aggctcgctg ttcttcactt agacgatatt
     8581 tgcgcatagc gttttctcac aggtgactta ctatgaccgt agcaaattct gcggctctgg
     8641 ctatgctcga gaaaattcca taaaatgcat ttcaaatata ctttataaat taaacaaaat
     8701 gagtaagaag atgcagagcg ataaagtgct caatttgccg gcaggctact ttggtattgt
     8761 gttggggacg atagggatgg gatttgcctg gcgctatgcc agccaggttt ggcaggtcag
     8821 ccactggtta ggggatgggc tggtgattct ggcgatgatc atctggggat tattaactag
     8881 cgcatttatt acccgactca tacgctttcc gcatagcgtg ctggcggaag ttcgccatcc
     8941 agtgctgagc agttttgtga gtttgtttcc ggcaacgacg atgctggtgg cgattggttt
     9001 tgttccgtgg tttcgcccac tggcggtgtg cctgttcagt tttggtgtcg tggttcagtt
     9061 ggcttatgcc gcctggcaaa ctgcgggatt atggcgcgga tctcaccctg aagaagctac
     9121 catgcctgga ctgtatctgc cgacagttgc caacaacttt atcagcgcaa tggcctgtgg
     9181 tgcgttgggc tacaccgacg ccggtctggt gtttttaggc gcaggcgttt tctcatggct
     9241 aagccttgaa ccggtgatct tgcagcgtct gcgcagttcg ggagaattac ccacggcact
     9301 gcggacatca ctcggcattc agctcgctcc tgcgctggtg gcctgtagtg cctggttgag
     9361 cgtcaacggc ggcgagggtg atacgctggc gaaaatgctt tttggttatg gactgctgca
     9421 actgctgttt atgctacgtc tgatgccatg gtatctctcc cagccattta atgcttcatt
     9481 ctggagtttc tcgttcggcg tatctgcact ggcaaccacc ggtttgcatc tggggagtgg
     9541 cagcgaaaat ggatttttcc atacgctggc ggtaccgctg tttatcttta ccaattttat
     9601 tattgcaata ctgctcatcc gtacttttgc gcttctgatg cagggaaaat tgttagtcag
     9661 aaccgagcgc gctgttttaa tgaaagcaga ggacaaagaa tgatcattcg tgacgaaaac
     9721 tattttactg ataaatatga attaacccgc acacactctg aagtactgga agcggtgaaa
     9781 gtggttaaac cgggtaaaac gctggatctg ggctgtggca atggtcgtaa cagtctttac
     9841 ctggcagcca atggttatga tgttgacgca tgggataaaa atgccatgag tatcgccaat
     9901 gtagagcgca ttaaatccat tgaaaatctg gataatttac acacccgagt cgttgatctg
     9961 aataacctca catttgatag acagtacgat tttattcttt cgactgtggt actgatgttc
    10021 cttgaggcta aaaccatccc agggctgatt gccaatatgc aacgttgcac taaacttggt
    10081 ggttacaacc tgattgtggc ggcgatggat accgctgatt atccatgtac cgtcggcttc
    10141 ccgtttgcct tcaaagaggg agaattacgt cgatattacg aaggctggga gagggtgaaa
    10201 tacaatgaag acgtcggcga gctgcaccgc accgacgcca acggtaatcg tattaaactg
    10261 cgtttcgcca cgatgctggc acgtaaaaaa tgacccgcta agaataaggc gcgtgaaaat
    10321 tccccacgct gagatgattt actgttcttc ttttcggtaa gcatattttt tatcgaaggg
    10381 atgtgaaatt aatcacagta gtcgaagttt tttagcagct taacttactg aaatttaact
    10441 actgatgatt gacttagccc ctttttcggc attgactatg tcgtctgaaa aggggctgaa
    10501 aaatttattt tcaccaacac tttttttgcc acaacacgaa gcggcgcttt ttgctataac
    10561 ttaaaaagta atataatcat ctcaggaaac tattcatgcg taccacatca tttgcgaaag
    10621 ttgcagcttt atgcggcttg ttggctctgt ctggttgtgc ctctaaaatc acccagccag
    10681 ataaatattc tggtttt
//
LOCUS       DADLFA010000140        10694 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000140 DADLFA010000000
VERSION     DADLFA010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10694
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.141"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            311..1609
                     /gene="kgtP"
                     /locus_tag="JQH65_003065"
     CDS             311..1609
                     /gene="kgtP"
                     /locus_tag="JQH65_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HAY6140398.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1606..1950)
                     /locus_tag="JQH65_003066"
     CDS             complement(1606..1950)
                     /locus_tag="JQH65_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708437.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HAY6140399.1"
                     /translation="MFFGGHVLRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAM
                     LSAAGNEYSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGA
                     STGYTVWQLTRH"
     gene            complement(1975..3330)
                     /gene="pssA"
                     /locus_tag="JQH65_003067"
     CDS             complement(1975..3330)
                     /gene="pssA"
                     /locus_tag="JQH65_003067"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HAY6140400.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYKAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENSGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRLFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3444..6104)
                     /gene="pat"
                     /locus_tag="JQH65_003068"
     CDS             complement(3444..6104)
                     /gene="pat"
                     /locus_tag="JQH65_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HAY6140401.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQRKMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AISNPLDLRDDASSEHYIKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RIHGLLVQSMANRAGAQELRVVVGHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6136..6834)
                     /locus_tag="JQH65_003069"
     CDS             complement(6136..6834)
                     /locus_tag="JQH65_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DTW domain-containing protein"
                     /protein_id="HAY6140402.1"
                     /translation="MTENTVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREALAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6903..7322)
                     /gene="trxC"
                     /locus_tag="JQH65_003070"
     CDS             complement(6903..7322)
                     /gene="trxC"
                     /locus_tag="JQH65_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HAY6140403.1"
                     /translation="MNTVCTHCQSINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7529..8566
                     /locus_tag="JQH65_003071"
     CDS             7529..8566
                     /locus_tag="JQH65_003071"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HAY6140404.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAELVQSITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8614..9303)
                     /gene="ung"
                     /locus_tag="JQH65_003072"
     CDS             complement(8614..9303)
                     /gene="ung"
                     /locus_tag="JQH65_003072"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAY6140405.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9608..9991
                     /gene="grcA"
                     /locus_tag="JQH65_003073"
     CDS             9608..9991
                     /gene="grcA"
                     /locus_tag="JQH65_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HAY6140406.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10047..10634)
                     /gene="eamB"
                     /locus_tag="JQH65_003074"
     CDS             complement(10047..10634)
                     /gene="eamB"
                     /locus_tag="JQH65_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HAY6140407.1"
                     /translation="MTPTLLSAFWTYTLITAMTPRPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
BASE COUNT         2663 a         2716 c         2745 g         2570 t
ORIGIN      
        1 aaaggccatc cgtcaggatg gcctttttgc attggcgcag aaaaaatgac tgatgcgacg
       61 ctgcgcgtct tatactccca catatgccag attcagcaac ggatgcggtt tccccaactt
      121 gcctacttcc atacgtgtcc tccttaccag aatttatcct taagctcctc aataaccatt
      181 ttcctgctaa ctaaattcat ggttaatgtt gcataataat acgtaacaaa tgtataacat
      241 tttctttacc aaaagaaata aacaaaagtg accaacaaaa gcatcggatt acggcaggag
      301 acataatggc atggctgaaa gtactgtaac ggcagacagc aaactgacaa gtagtgatac
      361 tcgtcgccgc atttgggcga ttgtgggggc ctcttcaggt aatctggtcg agtggttcga
      421 tttctatgtc tactcgttct gttcactcta cttcgcccac attttcttcc cttccgggaa
      481 cacgacgact caactactac aaacagcagg tgtttttgct gcgggattcc tgatgcgccc
      541 aataggcggt tggctatttg gccgcatagc cgataaacat ggtcgcaaaa aatcgatgct
      601 gttatcggtg tgtatgatgt gtttcggatc gctggttatc gcctgcctcc caggttatga
      661 aactataggt acgtgggctc cggcattatt gcttctcgcg cgtttatttc agggattatc
      721 cgttggcgga gaatatggca ccagcgccac ctatatgagt gaagttgccg ttgaagggcg
      781 caaaggtttt tacgcatcat ttcagtatgt gacgttgatc ggcggacaac tgctagccct
      841 actggttgtc gtggttttac aacacaccat ggaagacgct gcactcagag agtggggatg
      901 gcgtattcct ttcgcgttag gagctgtgtt agctgttgtg gcgttgtggt tacgtcgtca
      961 attagatgaa acttcgcaac aagaaacgcg cgctttaaaa gaagctggat ctctgaaagg
     1021 attatggcgc aatcgccgtg cattcatcat ggttctcggt tttaccgctg cgggctccct
     1081 ttgtttctat accttcacca cttatatgca gaagtatctg gtaaatactg cgggaatgca
     1141 tgccaacgtg gcgagtggca ttatgactgc cgcattgttt gtattcatgc ttattcaacc
     1201 actcattggc gcgctgtcgg ataagattgg tcgccgtacc tcaatgttat gtttcggttc
     1261 gctggcagcc atttttaccg ttcctattct ctcagcattg caaaacgttt cctcgcctta
     1321 tgccgctttt ggtctggtga tgtgtgccct gctgatagtg agtttttata catcaatcag
     1381 tggaatactg aaggctgaga tgttcccggc acaggttcgc gcattaggcg ttggtctgtc
     1441 atatgcggtc gctaatgcta tatttggtgg ttcggcggag tacgtagcgt tgtcgctgaa
     1501 atcaatagga atggaaacag ccttcttctg gtatgtgacc ttgatggccg tggtggcgtt
     1561 tctggtttct ttgatgctac atcgcaaagg gaaggggatg cgtctttagt gacgggtcag
     1621 ttgccagacg gtatagccgg tgcttgcacc ggcgacatcc caggccaaat ccttccagct
     1681 ccagccgctc ccttcggggc ggctatccca aagctctttt gacgccccca aactgacaga
     1741 gaacatcaat ccaaacatgg cactgcgatc ccggctcatc ccctgatgct gtgaatattc
     1801 atttccggcg gcggaaagca tcgccgaggc gataaagtgt tgagctttat cctgcccgct
     1861 ccagctatcg ttagccatat ggctgcatcc agaaagtaac aatagcgaac agacaaaaag
     1921 aatacgcaaa acgtggcctc caaaaaacaa accccgtaca ggacggggtt gtgattacag
     1981 gatgcggcta attaatcggt cgatgcggat acggcgcaaa cggcggatga gtttacgaac
     2041 cttcaccgga taatcggcaa tactttgcag atcgcgatag tgcttaacga tggtggtatg
     2101 ctcgcggatc agctccagtt ctttctctcg ctgcggcgca agctccagtt gtggatcgtg
     2161 gatcaaaatg gcgttttcca gatccagacg ccaggcgcgc gggttcaggt tattaccggt
     2221 gatcaacatc cacttatcat caacccacat ccctttcagg tgataggtgt tgtcgtcatc
     2281 tttccataac cgaaccacta gctggtcagt attgacgtaa tactgcaaac ggctcaggaa
     2341 aagacgcaga ttgatctcat agagataagg caatgcgcca attatcttga aaggttcatc
     2401 ttccggaatg tagaagtcgt tcgcggtttt atcaccaaca ataatttcga cctttttccc
     2461 ttcgcgcagc aactggataa tattgcgcac aaggattgct ggcaggttga agtatggcgt
     2521 acagatggtt agtttctgct cggcacaagg cataagatgg aaaatggtct tgttcaacag
     2581 actcgatttc cccagcccca ctagcggcgt tacggaaagc tgatcgttgt cggcatcgcc
     2641 ctggaaatga taagcggcat cacgcagctc ctggcggaac agacgaatat cgttcttgat
     2701 ttccgggctt tttggccgat taacatcatc cagacgatta acgccgcggc cattcataat
     2761 attctgtgta acccattcaa acataatgtc tgacatctta cggttacgga taagatgata
     2821 acggtcgtag cgatatttat cgtgctgatg caggtaaaca tcgttcaggc tggcaccgct
     2881 ataaagtacg ctatcgtcga tgataaagcc tttaaagtgc agaacaccaa gggcttcacg
     2941 ggtattgatt ggaacgccat aaaccggaac atctacgccc gaattttcct gcgccatgcg
     3001 gcagtaccag tcagcgttag tgttagatgc cgcagcgcca atgcgtccac gttgtgcacg
     3061 atgccagtcg accagcaccc gcacatccag ttccggacgc tgccttttag ctttatacaa
     3121 cgcgttcaga atgcctttgc caccgtcatc ctgttcgaga tacagggcga caatgcaaat
     3181 gcgctgcttc gcgctggcta ttttttccag cagcgtctcc cggaagtcgg cgggagcgta
     3241 aaagaaatcg acatcatcaa ctgattgaga aatcttgggt agttgggcaa ggtgttgttg
     3301 atgtttatta cgcttaaatt ttgacaacat cacagtgcat ttcttctctg ttcattgaag
     3361 gatcctctgt gcaatgcaga cgacataagc gggcaataat aacaccagtc ccgattaagt
     3421 agtcaacatt tccagtacct tactcatgat tcctcgcgtt gggcaagatt tagcgtaagc
     3481 ccaacgatcc cctcttcgag ctggatatca acgttaaacc cgagcttgcg ggctagcgcc
     3541 accatgccac gattgtttgg catcgtaata ccattcagac gttgtagtcc gtgatctcgc
     3601 gtataggtaa tcaacttttc cattaagcgt cgacctaagc ctaacccttt gagatccgag
     3661 cgaaccagca cagcaaattc ggcatcgatg ttatcaggat cggaaatcgc acgcgtgacg
     3721 ccgaggatct cttccgtttg atcaatacgt cgtaccgcta caaacgccat ttcccgatcg
     3781 tagtcgatct gtgtcatgtt ggctaaatct tcatgggtaa attcgttgat ctcgctaaag
     3841 tagcggtaat aaagatcttc tttggtgact cgcgaaatga attgctgaag ttgtggctca
     3901 tcttctggca aaatcgggcg gaacaagcag cgttcaccgt ttttcaattc tacccattct
     3961 tccagctgat gcggataagg gcgcactgcc agccgactct cgttatcgcc ttcaaacggc
     4021 gagatatcca gcgtgacatc cagcgcggta aattcactgc cagaagccag caaaggatga
     4081 atatccagac gctgaatttc cgggcaatcg acaatcaagt tggaaacctg caccagaagc
     4141 tggctcaagc ctgcaacatc caatgggcgt agcgcactgc gcgcacgaat ctttttactt
     4201 ttgatcccct gaataaccag atagcgggcc aggttcatgt tcagcggcgg cagtgcgacg
     4261 acggcttgat cttcaggacg ccactccaca ccgccttcac ccagcatgat caacggcccg
     4321 aaaaccggat cgtgcccaac cacaacccgc aactcctgag cgccagcacg gttagccata
     4381 ctttgcacca acaggccgtg gatccgcgcc tgcggccagg ccatttttac gcgatcgaaa
     4441 atggcgtttg ccgcttgctg gacttcattg gctgtacgca agtacagcat gacgccctga
     4501 acttccgatt tatgtggaat atccggcgaa cgcaatttca gcgccaccgg ataaccaatc
     4561 tgttcggcaa tatgcaccgc ttcggtgcta tcgctggcaa tccaggtagg gagcgtgttc
     4621 atgccatacg cttgcaggat gggctgaact tcatgggtat cgagcgacgt agccccttcg
     4681 gcaatcgcct gttgcaacag aagatgcgct tctgcggtat tggaagtcag attgctgggc
     4741 aacgccggcg tttcgcgtag ttgcttctga ttacgccggt actccaccat atgcataaaa
     4801 gcagtgatgg ttccttccgg ggtacggtag gtcggcagcc cggcttcgct gaataaacgt
     4861 cgtgcctctt gcgaggagtg ctcgccgcac cagttcgtca gcaaagagac atatttgctg
     4921 cggggatgat gctttaccgc ttcaattaat acttgcgcgc tttctgttgc gggagcagcg
     4981 gcgctgggcg aatgaataac catcagcgcg tcaaaatcct ggctgtggag cagaatatcc
     5041 agcgttttaa tatagtgctc actgctggcg tcatcgcgta gatcgagcgg gttagatatt
     5101 gccacatgtt ctggcagtgc atcgcgcagt ttctggcagg tttcttcgct tagcgttgcc
     5161 agcttgccat tgcgtgacca taaggcatcc agcgccagcg cggcaggcgc agcaccgttg
     5221 ctgataatca tcagccggtc gccacgtagc gggcgcatat ggctaagggt ttccaccgcc
     5281 gaaaacagct cgtgggtgtc ctgtacccgc aacaaaccgg cacgctgaat agccgcatcc
     5341 catgccgggt ccattcctgc cgtggtgttg agcagtcgct gtgccgccgg gctacgtccg
     5401 cttttaatca ccagaatcgg tttattacgc gaggcactac gggccgccga aacaaagcgt
     5461 cgcgcgtcgc ttaactgttc gagatagagc aggatggcgc tggttttact gtcgcgtgcc
     5521 agatagtcaa gcaattcatc aacgtcgata tccaggctgt cgccgagcgc aataaagtag
     5581 gaaaagccca tcttacgctg ttgcgcccag tcgaggatgg tgttggagac ggcagccgat
     5641 tgcgaaataa acgccagctt gccgcgttta atcggcacag gcgaaaagct ggcattcaga
     5701 ccttgccagg gagccagtaa acccagactg tttggtccaa gcaggcgcat gttatggcgc
     5761 agggcgcagg cgcggagatc ttcgtgttgc gatgccgggg cggaaagaat aatgcaggtt
     5821 ttacagcctt tctcgccgag ctcttccaga agagcaagat tacggctggc attggtacat
     5881 aaaaccgcaa ggtcgggtgt aaagggcaag ctggcaatat ccggccaggc caacacaccc
     5941 aacaccgctt tccaggctgg cgtcaccggg agtaccggtc cgttaaagcc tcccgccagc
     6001 aggttacgca tcatcaggta acctgcgcga ttgggtttca tcgacgcgcc aattaccgct
     6061 atcgattttg gtcgcagtag tgcttccagt cctcgctgac tcataccggt ctcccgcaga
     6121 agtgaccgaa tgattttaaa cgctttctaa ctgttctgct gtgatgctac ccagatgttg
     6181 cgttttccct gccagatagc gtgttttaaa gcgggtaaaa tgctcgccta accctgctgc
     6241 cgccccggta tcgccggcca tatctaacag tgcgatggct acctcggcag tacaatattg
     6301 gccttcagcc agggcttcac gcaggcgata ggcagaaagc cgggaaagat cgacggaaat
     6361 gacgggaaga ttatccagat acggactttt acgaaacatc ttgcgagctt ccggccaggt
     6421 accatcgagc atgataaaca gcggtggctt accggcaggt ggtgtgaaga tcacttcccg
     6481 ttgctcatca gcatacgagg cgggaaagac caccattggc tgataatccg ggttttgtac
     6541 cagatccagc aaatcctgcg agggttcggt acgcgaccat tgaaacgcaa cggtatcagg
     6601 caaaatatca gcaatcagac gcccggtatt actgggcttc attggctcgg tgtcgaacat
     6661 cagcaaacag aagcgacttt ttgcttgtgc tggggtaatt gtcgaacaga gacataattt
     6721 ctctggcaaa agacagcgtt ggcagcgacg aacgcgatta ccgcgggcaa gaaaaggacg
     6781 tgttgcgcgc gcaatacgct cggcgcgtaa ctggagaaca gtgttttcgg tcataagaga
     6841 gcgtcgaaaa aacgccattg tcgcagagga gaaaacgggg cacaagatgc gccccggtaa
     6901 gattaaagag attcgttcag ccagctatcg aacggagctt tcggtactgc gccattaagc
     6961 atgtcgacaa cctgaccgtt tttgaaaatc atgatcgtcg gtatactacg aattccaaag
     7021 cgactgctca attcacgttc agcttcggta ttcactttca caaagcgcac tttaccgcta
     7081 cgctcttgcg cgacatcttc aaaaattggt gcgaaattac ggcaggggcc gcaccacggt
     7141 gcccagaagt cgatcaccac aggtagatca tccttcagca atttgtcgag tgtttcaccg
     7201 gtcgcattaa tcacctctcc gtcaaacaag tcgtgaccgc agcgtccgca ttttgccgca
     7261 tcttcgatcc gatcgtcggg aatgcgattg atggactgac aatgggtaca aacggtattc
     7321 ataactaacc tcgggatgag taaggagact tatcggcaac gacgccggta tgtttctaat
     7381 atgttacata ttatcgggga cattgttcca gacaacaatc tgttttattc aataggtatg
     7441 atagtcgcag gctttggtac gaaatcatgg agaaccgcgc ggacttcggg taatctgcgc
     7501 gcttcgcgca gcgctggtgg agaaaagcat gaacgatgaa atgaaaggta aaagcggcaa
     7561 ggtcaaagtg atgtatgtcc gcagtgatga tgattctgat aaacgtaccc acaacccgcg
     7621 taccgggaaa gggggcgggc gtccaggaaa atctcgtgct gacggtggcc gtcgccccgc
     7681 ccgcgatgac aaacagagtc agccccgtga ccgcaagtgg gaagattcgc cgtggcgcac
     7741 ggtttcccgc gcgccgggtg atgagacgcc ggaaaaggcc gatcacggtg gcatcagtgg
     7801 taaaagtttt attgatccgg aagtgttgcg tcgtcagcgt gcggaagaaa cccgcgtcta
     7861 cggcgaaaac gcctgtcagg cactgttcca gagccgtccg gaagcaattg ttcgcgcctg
     7921 gtttatccag agcgtaacgc cacgttttaa agaagccttg cgctggatgg cggcaaaccg
     7981 caaagcgtac catgtggtgg atgaagctga attgacaaaa gcgtcaggca cggaacatca
     8041 cggcggcgtt tgcttcctga tcaaaaagcg taacggtaca accgtgcagc agtgggtaag
     8101 ccaggcaggc gcgcaggatt gcgttctggc actggaaaac gaatctaacc cgcataacct
     8161 gggtggcatg atgcgcagct gcgcgcactt cggcgtgaaa ggtgtcgtgg tgcaggatgc
     8221 ggcactgctg gagtcggggg cggctatccg taccgcagaa ggcggcgcag agctcgttca
     8281 gtcgattact ggcgacaaca ttgttaacgt gctggatgat ttccgtcagg caggttacac
     8341 cgtagtgacc acttccagcg agcagggtaa accgctgttc aaaactagtc tgccagcgaa
     8401 aatggtactg gtgctgggtc aggaatatga agggttaccg gatgccgcac gcgatccgaa
     8461 cgatctgcgc gtgaagattg acggtacggg caacgttgcc gggctgaata tctctgtcgc
     8521 aaccggcgtt ttgcttggtg aatggtggcg tcagaataaa gcctgataaa tcagccgggt
     8581 ggcaactctg ccatccggca tttccccgca aatttactca ctctctgccg gtaatactgg
     8641 catccagtca atcggcgtct cgccacgttg ttccagccac tgatttgcca gcacaaaatg
     8701 gttgcagcca aagaatccac gatgcgccga aagcggcgac ggatgcggtg ctttcagtac
     8761 atgatggcgt tgcttatcta taatcgcccc tttcttttgc gcatgtgatc cccacaacaa
     8821 aaacaccacg ccttcgcggt gctggttaat caggctgatg actttatcag taaacgtctc
     8881 ccaaccgagg ctggcgtggg aatgcgcctg acctgcgcga accgtcaaca cagtattgag
     8941 cagcagaacg ccctgacgcg cccagctttc gagatagcca tgattagggc gggtgaagcc
     9001 cggaatagta ttttccagct ctttatacat attcaataat gacggaggaa tggcaatgcc
     9061 gggacgaacg gaaaatgcca gaccatgcgc ctgtcccggt ccgtgataag gatcctggcc
     9121 gagaatcacc actttaacgt cacccaactc ggtaaagcgg aacgcgttaa agacatcttt
     9181 ttgtggtgga tagatagtga cgccggactg ccgctcgctg gcgacggtct gaagggtatt
     9241 aagaaaataa ggttgttgct tctcttcagc cagcacgtca tgccaggtta attcgttagc
     9301 catctcgctc tcctgcgaat cttcaatccg cctagcttaa ctgcttcttt ctacagaaca
     9361 aaatcatgct cattttttag acttaaacga gtgcaaaaaa atgtaaattg gtaaaatttc
     9421 acacccgcat aagttgatgt aaaacaataa aatccattta cgaccacaat cgatgtgtgg
     9481 tttttattga tttaaatcaa agaatcaagg gtggttgagg agtatatata cactcaagca
     9541 acaatggttt taccaattag ccgcgacagg ctgaacaaat caaataattt tgccggggag
     9601 gcatcacatg attacaggta tccagattac taaagccgct aacgacgatc tgctgaactc
     9661 tttctggctg ctggacagcg aaaaaggcga agcacgttgc atcgttgcaa aagcaggtta
     9721 tgcagaagat gaagtggttg cagtaagcaa actgggtgac attgaatacc gtgaagttcc
     9781 agtagaagtg aaaccagaag ttcgcgttga aggtggtcaa cacctgaacg ttaacgttct
     9841 gcgtcgtgaa actctggaag atgcagttaa gcatccggaa aaatatccgc agctgaccat
     9901 ccgtgtatcc ggttatgcag ttcgctttaa ctctctgact ccggaacagc agcgcgacgt
     9961 tatcgctcgt acctttactg aaagcctgta aggcttaagt agcaagttac taagcggaag
    10021 agggcgacct cttccgcttt ttttcgttaa tagaaaatgc gtaccgcgca atagaccagc
    10081 aacagggcaa gcacgatatt taactggcga ccatactggc gaaacaatcg ctgaaacaga
    10141 tgccccgcca gcgcccagca cacattgcca aacgtcccaa tcatcgccag caaaacgctg
    10201 acgccaacta cccagcttag cgcctgtgtt tgcggcagaa caaacgtcga cagtgccgta
    10261 acaccgtaca aaatgatttt gacgttcaca aactgcaaag caaagctggc ccaaaagctg
    10321 attggttttg cctgaagtcc gtcttccttt gttgggctgg tggcgatttt ccacgccagc
    10381 cagacaatat atgccgcccc cgcccaactc aaaaggtgta ccgctgccgg gtcaatcact
    10441 gccagtgaaa atgaaatgcc cgcacacagt aacatcacaa tcaaaaatcc cagactcatc
    10501 cctgccagca cgcgggtact ttgacgaaat ccatgcgacg tagcagagct aagggcgaga
    10561 atattgttcg gtcttggcgt catagcggta atcagggtgt aagtccaaaa agcacttaaa
    10621 agggtcggtg tcacttgtta tctcctctgt tttggtagac agtacctaga ctggccccct
    10681 gaatctccag acaa
//
LOCUS       DADLFA010000141        10630 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000141 DADLFA010000000
VERSION     DADLFA010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10630
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.142"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..254)
                     /locus_tag="JQH65_003075"
     CDS             complement(<1..254)
                     /locus_tag="JQH65_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HAY6140408.1"
                     /translation="MAGYLSWLFPRCKISPKLNGTAPHFGDEMFALVLFVCYLDGGCE
                     DIVVDVYNTEQQCLYSMSDQRIRQGGCFPIEDFIDGFWRPA"
     gene            complement(275..511)
                     /locus_tag="JQH65_003076"
     CDS             complement(275..511)
                     /locus_tag="JQH65_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HAY6140409.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(629..2068)
                     /gene="rsmF"
                     /locus_tag="JQH65_003077"
     CDS             complement(629..2068)
                     /gene="rsmF"
                     /locus_tag="JQH65_003077"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HAY6140410.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVDIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(2148..4781)
                     /gene="yebT"
                     /locus_tag="JQH65_003078"
     CDS             complement(2148..4781)
                     /gene="yebT"
                     /locus_tag="JQH65_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HAY6140411.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RSNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGTGKEQDHFVALDTQPKYRLNNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDTGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVSGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISATGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(4750..6033)
                     /gene="yebS"
                     /locus_tag="JQH65_003079"
     CDS             complement(4750..6033)
                     /gene="yebS"
                     /locus_tag="JQH65_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HAY6140412.1"
                     /translation="MALNTPQITPTKKITVRSIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            6163..6660
                     /locus_tag="JQH65_003080"
     CDS             6163..6660
                     /locus_tag="JQH65_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAY6140413.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6757..7455
                     /gene="proQ"
                     /locus_tag="JQH65_003081"
     CDS             6757..7455
                     /gene="proQ"
                     /locus_tag="JQH65_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HAY6140414.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7475..9523
                     /gene="prc"
                     /locus_tag="JQH65_003082"
     CDS             7475..9523
                     /gene="prc"
                     /locus_tag="JQH65_003082"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HAY6140415.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9715..10596
                     /gene="htpX"
                     /locus_tag="JQH65_003083"
     CDS             9715..10596
                     /gene="htpX"
                     /locus_tag="JQH65_003083"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HAY6140416.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
BASE COUNT         2715 a         2686 c         2757 g         2472 t
ORIGIN      
        1 gcaggtcgcc agaaaccatc tataaaatcc tcaatcggaa aacaaccgcc ctggcggatc
       61 cgttgatcgc tcatagaata aagacactgc tgttccgtgt tgtagacatc cacaacaata
      121 tcttcacaac cgccatccag gtagcaaaca aaaagtacca gcgcgaacat ttcatccccg
      181 aagtgtggtg ccgtaccgtt aagtttagga gagattttac aacgggggaa taaccaggac
      241 aaataacccg ccatgccgac gggttctttt tggatcaggc aagacgcata atccaggcat
      301 cagattgttg atcgtaacgg gtacggtaaa ggacagagtg ttcgccattt agcagggcag
      361 ggatactggt ttcagcatcc caggcatcga gttgcataca taaatcgggg tcagatttac
      421 aaggaatggt taacgttcga tcacccggcg attcaccgtg taattcgccg tcgtcgattt
      481 caaaagcgcc tatgcgcaca ctggttttca tcttcgtgct ctccatttag ctgccggttg
      541 tggtacgacc agtccgatcg caccattttt cagcctagtt gaggcagccg aatgtgccag
      601 tcaaaaagtg cgcttttttt gtgcgctgtc aggcgttacc ggtaaaaagt ttcccatcgc
      661 gcaccagttc acgcggatag ctgtttttca atcgcgaacc aatccgtttg gctaaaccaa
      721 tcggttgatg ctggaaagta accaacacgt catccgccac tggcgcggct tgcgggtaaa
      781 catcgcgccc gcgataccac tcctccgctt cctgcggtgt cagttcaaaa gcgttcacat
      841 tgtcgggggt ggcaagggca ataactgctt catgctgcca gcgataacct ttgttgtgcg
      901 tttcggcaag tttaatcccc aagcgagaaa atcggacttt accgatcagg gcttcaatgt
      961 ccaccgggaa caaccacagt tctttgtcac gctgccagag gcgcaggttt tcatcccagt
     1021 ttaagccaac acctgcagcc gcctggcgaa tttgtccagc ttcgcgatct ttcaccgggc
     1081 tgaacggaaa attaccgact ttgtatttgg gggcgggtaa ggcgggaatc gcctgagttt
     1141 tacgcagacg agcaacgaag aagccttcgc agtcgtaaat ttgtgggaaa acatgcaaaa
     1201 agccttcttc ggtcagcgct ttgtttgcac cagggaagag atcgccaagt ggtaaaaact
     1261 ctactgcgtc ggggtaagtc tctttcagcc acaggcaaac ggcttcgttt tcttcctggt
     1321 ttaaggtaca ggtcgagtaa accagcgtac caccaggacg taatgcatga aaggcgctgt
     1381 cgataagctc ccgttgtgta gctgcgattt cctgattgct ttctggtgac cagtttttta
     1441 gcgcatcggg atctttacgc accacgcctt cgccggagca gggagcgtcc agcaaaatgg
     1501 catcaaacat ttctggcact gccgcaccaa acacgcggcc atcaaaatgt gtgagcgcaa
     1561 cattactgat gccacagcgg ctgatattgg catgtaacac ttttacccga ctggcggaaa
     1621 actcattggc aaggattgcc ccttcgttat tcatccgcgc ggcaatttgc gtcgtttttg
     1681 agcctggcgc agcagcgaca tccatcaccc gctgtggtgc attaccgtca gcaaacaagg
     1741 cggcgacggg caacattgaa ctggcttcct gaatataaaa caggccactt aaatgctcgg
     1801 cggtactacc caatggcaat gcatcttcat tgtcgcgttc aatccagaaa ccttcttcac
     1861 accacggaat tggcgtaagc gtccagccat aaggagcggt taattgcagg aaatcagcaa
     1921 cggagatttt cagcgtatta acgcgaatgc tgcggcgcaa cgggcgctga caggcggcaa
     1981 gaaaatcatc aaatgagagc gtcgaaggca tagcttcgcg catttgtgtc agaaacgcgt
     2041 ccgggaaata aacggtgtgt tgggccacga gcatgtacca ctggcaaaaa aacaggcgcg
     2101 cagtttagca taaacgctcc ggcgcaggca cgccggagca gtgggcatta tttgggaagc
     2161 gcagttcccc attcacgcca ctctttcggt tcactttcct gcaacaggaa gtgtttgcct
     2221 tcctgggctt tcggtgccag tggcgtaccc ggaggcgtgg cgaaggcgat gccgccacgg
     2281 ataaactggt taaaggtgcc ggttttcact acgccgcccg tcagaccaaa gtccagactg
     2341 taacccgatg ccaaccagaa gacggaattg ttacgcacca ggtgttgata gcgtttactg
     2401 atgcgcatcg caatcatcac gcgatctgac aatgtcccca gcgtcattcc ggtaaccgta
     2461 cctacttcca gaccacggaa cagcacaggc gtaccaatac ctaacgaacc ggcttccggt
     2521 gcttcgacaa taatgcttaa gccatccagg taacgcgaat cagtaatagt agcttcttgc
     2581 aactcaaagt cacggcgagg attaccccga cctggttcga cattgatata cggctggagg
     2641 atagtatcta gatgctcaac gcccgtggct gaaatttgcg gtgtgaccac cgagaagcgc
     2701 gtaccgccgc gggcgaaggt ctggacatac tccggataga gcaccgcctt tgcctgtact
     2761 tcattgcgcg cggtaatcag atccagcgtc tggatttgcc cgatatcaat accgagatag
     2821 cgaattggca tcccgaccgc cagttttccg gcatcgaaag cgtgaagcgt aatctgccca
     2881 ctaaccgcac gggccgctgt ttcggaagca tacagaattc gtttgtcgcc tttacgctga
     2941 ctggcgctgg caccgctgag gttatcgaag ctaatggctc cctttaatgc tctggagagc
     3001 ggggatgcct gtacggtcag gccactacca ttcagctgaa ctttcgcccc gccttctgcc
     3061 cagaacacgc tattgctggt cagaaggttg cgatactccg gcttaatatg cagatcgata
     3121 tcaaacgcgt tagctcgcgg acgcacggta atcacttcac caacttcaaa tttacggtac
     3181 agcactaccg atcctgcctg cacatccggc agcgtctctg cactcaaact cacggttgtg
     3241 gtgggtaaat cgctaaggct gttctccagc gctttttcca gattggcata cagtggatag
     3301 ctggctttca tctcaccttt atcgcctggc agaatacgta tcccgccgtt aatccattct
     3361 gaggcgctgg caccaagaaa ctcaacgcca tccagcccca ccttcacgtc gacacggctg
     3421 ttgacgacaa atttgctatc gccttttacc agttcgcgat gctgaggctc gatggcgacg
     3481 gtaaaggtaa cgcctttgct ggtgagttta cgatcaataa cctggcctac ctgcacgccg
     3541 tgaagaatga gcggctgacc cgtatcaata ccgtaacttt ccggcgcggt cagggtcagc
     3601 gtcagaacat caggttcatg cagcagtgct ttttcgcctg gcacaacaac gaactctttg
     3661 cgtggctcgc catcgccggg taccaactcg aaggttttgc cagtcagcag ggcgctgaga
     3721 ttggcatcgc taagggataa tttcggatta cgtaattcga tgcgggtatt ttcacgaagc
     3781 agggtaacga cgctgggatc aactgtcatt tccccagtga ctttaccacc aggatttaaa
     3841 tccagtttag ttagctgtcc gacttccagt ccctgataca ttaatggcgt cgagtcggcg
     3901 gttaatccgg ccccactcgg cagttccagt ttgattatta cgccacgctg gctatgggcg
     3961 aggtcttcat acagaccaaa ggtatcttcc gcctcggcag gtttcgactc ttctggtgaa
     4021 tcgaaggcaa tcgcaccgtt aaccagtgcc gccaggcttt ccagtttcac cttcgcgcca
     4081 ctgatactga cattggcatc aacgccggaa acgttccaga aacggctacc ttttttcacc
     4141 agatcggtaa aacgccgctc gatcaggaca tcaatcacca cgccttgttt gttgggattg
     4201 atggcatagt cgtagacttt tcccaccggg atcttgcgga aatagaccaa tgaaccgctg
     4261 ttcagcgaac cgagatcggg ggcttgcagg tggatcatca gatcgccatt gttcagccga
     4321 tatttcggtt gggtatcgag tgcgacaaag tgatcctgct ctttacctgt acccggcatc
     4381 atgccgatat agttcccacc gacgagggcg tccagcccgg agacacctgc caacgatgct
     4441 tttggcgtca ccagccagaa ttgagtctct tcgcgcagcg catctttcat atcggacttg
     4501 atgctgacct tgacttcaat cttacgaaga tcgtcgctga ggctgatatc ctgcactgtt
     4561 ccgacttcaa cgccctgata acgaacaggc gtacggcccg ggacaatacc atccgccgac
     4621 ataaagtcga tggtgacggt attacttcgg tcctgataac tgtcccaaat cagccaaccg
     4681 gcaatcatta gcgcgatgaa aggcagcagc cagaaaggtg agatacggcg tttattttta
     4741 atctgcgctt cagtcgtcga agcgggcgtt tcctgactca tgtgcatccc aaagtaagcg
     4801 gctgtccagc cattccacag caagaatagt caaaattacg gctgcgccaa aataaaacgc
     4861 agccggtccc atagtaaaag cgaggatctg atcgcgatta atcagcgaca tggttaaaga
     4921 tatgacaaac aggtcgagca tcgaccagcg tccaatccag gtcaccatcc gcagtaacag
     4981 aatgcgtgtg cgtaaacctt gctggcattt aaaatgaatg ctcaacagta aagtgaacat
     5041 gacgatcact ttagtaaacg gtaccagaat actggcgata aacacgattc cggcgaccgc
     5101 aatgttgcta cttgccagcg acataatccc ggaaagaatc gtatcttcct gccggccacc
     5161 attcagataa atgatagaaa taggcaatag gttggctggt aacaacaaaa cgattgacgc
     5221 taacagcgcc gcccagcatt tttgcaaact atgacgacga cgcaggcgta gcgggatatg
     5281 gcaacgcggg cagcgaccac gctgatcagg atagccggta aaatggcacc caagacagac
     5341 acgaagtttc tcgtccctac gcgtagcggg gcgctgcgga taatatcgct cccacagctc
     5401 ctcaacatta agatgtgaca acgtcaccgt cgttaaaatc accaacgcca caaaagagaa
     5461 caagccgaca cccgcctgga tatgggcata atcctgtacc tttatagaag caacgccaat
     5521 gccgaccagg tagatatcca gcattaccca ctctttaaga cgctcaagca tcagcagtac
     5581 cggacgtaaa ttcattccca gtcggttacc aaaccataaa tatgctatgg aggacaccag
     5641 aatgaggggg gcaccaataa cgcagaaaaa gaccatcgcc cccgttatcg tatcgccctg
     5701 tttggtcatt tgccagatgc cttgcatgac gtttgcgtcg atacgaatac ctaacagcca
     5761 gatatgcaac agcggttcgc cccaggcaaa cggcatcagc aacagcatgg tgaaagccat
     5821 tgccgccagg cgcgttagcg accagtcgcg cccgtcgcga atttttgcct gacagcgcgg
     5881 acaataggca ctctgatgag aatttatctc gggcaggcta aacagcatgt cacattgcgg
     5941 gcaacgttgg taatcaccac gcggcagttc ctcgccgatt gacctcactg ttatcttttt
     6001 tgtcggcgta atttgtggtg tgttaagagc catgacgtgc caaatgtaat aagaattaac
     6061 attaatctta acccatgatg ggaatgatct tgagcattaa cgcatttaat gcttatttta
     6121 ataggctggc agttccaggt aagacaaaca agtgattata taatgaacaa aacagaattt
     6181 tacgcggatt taaatcgcga ctttaacgcg ctgatggcgg gagaaaccag ttttctggca
     6241 acgcttgcga acaccagtgc gttgttatac gagcgtctca ctgacgtaaa ctgggcaggt
     6301 ttttatttgc ttgaggacga tacactggta ctcggaccat ttcagggcaa aattgcctgt
     6361 gtccggatac ccgtcgggcg cggggtgtgc ggcactgcgg ttgcccgcaa tcaagtgcag
     6421 cgtatcgagg atgttcacgc gtttgacggg catattgcct gtgatgcggc gagtaattct
     6481 gaaattgttc tgccgctggt ggtgaaaaat cagattattg gtgttcttga catcgatagt
     6541 accgtcttcg gtcgctttac agacgaggac gagcaaggct tacgtcagct tgtggcacag
     6601 cttgaaaaag tgcttgcaac gacggattac aaaaaattct ttgcgagcgt cgcaggataa
     6661 tcaacggata acgtagcatt tactggtagc gtcattataa tgacgcctgt tcatgcctgc
     6721 gcttgttggc tacgtccgtt gtaatcagga aatttcatgg aaaatcaacc taagttgaat
     6781 agcagtaaag aagtaatcgc gtttctggcc gaacgttttc cccactgttt cagtgcggaa
     6841 ggtgaagcgc gtccgctgaa aatcggtatt tttcaggatt tggtcgatcg tgttgccggg
     6901 gaaatgaacc tgagcaaaac gcaattacga tccgctttac gtctctacac ttcgagctgg
     6961 cgttatcttt acggtgttaa acccggcgca acgcgtgtcg atcttgacgg caacccatgc
     7021 ggtgagctgg acgagcaaca tgtagagcat gctcgcaagc agcttgaaga agcgaaagcg
     7081 cgtgttcagg cacagcgtgc tgaacagcag gcgaaaaaac gcgaagctgc cgcagctgct
     7141 ggtgagaaag aagacgcacc gcgccgcgaa cgcaagccac gtccgaccac gccacgccgc
     7201 aaagaaggcg ctgaacgtaa acctcgtgcg caaaagccgg tagagaaagc gccaaaaaca
     7261 gtaaaagcac ctcgcgaaga acagcacacc ccggtttctg acatttcagc tctgactgtc
     7321 ggacaagctc tgaaggtgaa agcgggtcaa aacgcgatgg atgccaccgt attagaaatc
     7381 accaaagacg gcgtccgcgt ccagctgaat tcgggtatgt ctttgattgt gcgcgcagaa
     7441 cacctggtgt tctgaaacgg aggccgggcc aggcatgaac atgtttttta ggcttaccgc
     7501 gttagctggc ctgcttgcaa tagcaggcca gaccttcgct gtagaagata tcacgcgtgc
     7561 tgatcaaatt ccggtattaa aggaagagac gcagcatgcg acggtaagtg agcgcgtaac
     7621 gtcgcgcttc acccgttctc attatcgcca gttcgacctc gatcaggcat tttcggccaa
     7681 aatctttgac cgctacctga atctgctcga ttacagccac aacgtgctgc tggcaagcga
     7741 tgttgaacag ttcgcgaaaa agaaaaccga gttaggcgat gaactgcgtt caggcaaact
     7801 cgacgttttc tacgatctct acaatctggc gcaaaagcgc cgttttgagc gttaccagta
     7861 cgctttgtcg gtactggaaa agccgatgga tttcaccggc aacgacactt ataaccttga
     7921 ccgcagcaaa gcgccctggc cgaaaaacga ggctgagttg aacgcgctgt gggacagtaa
     7981 agtcaaattc gacgagttaa gcctgaagct ggcaggaaaa acggataaag aaattcgtga
     8041 aaccctgaca cgccgctaca aatttgccat tcgtcgtctg gcgcaaacca acagcgaaga
     8101 tgttttctcg ctggcaatga cggcgtttgc gcgtgaaatc gacccgcata ccaactatct
     8161 ttccccgcgt aataccgaac agttcaacac tgaaatgagt ttgtcgctgg aaggtattgg
     8221 cgcagtgctg caaatggatg atgactacac cgttatcaat tcgatggtgg caggtggtcc
     8281 ggcagcgaag agtaaagcta tcagcgttgg tgacaaaatt gtcggtgttg gtcaaacagg
     8341 caagccgatg gttgacgtga ttggctggcg tcttgatgat gtggttgcct taattaaagg
     8401 gccgaagggc agtaaagttc gtctggaaat tttacctgct ggtaaaggga ccaagacccg
     8461 cactgtaaca ttgacccgtg aacgtattcg tctcgaagac cgcgcggtta aaatgtcggt
     8521 gaagaccgtc ggtaaagaga aagtcggcgt gctggatatt ccgggcttct atgtgggttt
     8581 gacagacgat gtcaaagttc aactgcagaa actggaaaaa cagaatgtca gcagtgtgat
     8641 catcgacctg cgtagcaatg gcggtggggc gttgactgaa gcggtatcgc tctcaggtct
     8701 gtttattcct gcgggtccca ttgttcaggt ccgcgataac aacggcaagg ttcgtgaaga
     8761 cagcgatacc gacgggcagg tgttctataa aggcccgctg gtggtactgg ttgaccgttt
     8821 cagtgcttcg gcttcagaaa tctttgccgc ggcaatgcag gattacggtc gtgcgctggt
     8881 tgtgggtgaa ccgacgtttg gtaaaggcac cgttcagcaa taccgttcat tgaaccgtat
     8941 ttacgatcag atgttacgtc ctgaatggcc agcgctgggt tctgtgcagt acacgatcca
     9001 gaaattctat cgcgttaacg gcggcagtac gcaacgtaaa ggcgtaacgc cagacatcat
     9061 catgccgacg ggtaatgaag aaacggaaac gggtgagaaa ttcgaagata acgcgctgcc
     9121 gtgggatagc attgatgccg cgacttatgt gaaatcaggc gatttaacgg cctttgggcc
     9181 ggagctgctg aaggaacata atgcgcgtat cgcgaaagat cctgagttcc agaacatcat
     9241 gaaggatatc gcgcgcttca acgctatgaa ggacaagcgc aatatcgttt ctctgaacta
     9301 cgctgtgcgt gagaaagaga ataatgaaga tgatgcgacg cgtctggcgc gtttgaacga
     9361 acgctttaaa cgcgaaggta aaccggagtt gaagaagctg gatgatctac cgaaagatta
     9421 ccaagagccg gatccttatc tggatgagac ggtgaatatc gcactcgatc tggcgaagct
     9481 tgaaaaagcc agacccgcgg aacaacccgc tcccgtcaag taatatcaat caggcacaag
     9541 aaattgtgcc tgatttttta acagcggcaa gatgccgtaa atcagatgct acaaaatgta
     9601 aagttgtgtc tttctggtga cttacgcact atccagactt gaaaatagtc gcgtaaccca
     9661 tacgatgtgg gtatcgcata ttgcgttttg ttaaactgag gtaaaaagaa aattatgatg
     9721 cgaatcgcgc tcttcctgct aacgaacctg gccgtaatgg tcgttttcgg gctggtactg
     9781 agcctgacag ggatacagtc gagcagcgtt caggggctga tgatcatggc cttgctgttc
     9841 ggttttggtg gttccttcgt ttcgcttctg atgtccaaat ggatggcatt acgatctgtt
     9901 ggcggggaag tgatcgagca accgcgtaac gaaagggaac gttggctggt caatactgta
     9961 gcaacccagg ctcgtcaggc agggatcgct atgccgcaag tggctatcta ccatgcgccg
    10021 gacatcaacg cttttgcaac cggtgcgcgc cgtgatgcct ctctggttgc tgtcagcacc
    10081 ggtttgctgc agaacatgag cccggatgaa gccgaggcgg taattgctca cgaaatcagc
    10141 cacatcgcca atggtgatat ggtcaccatg acgctgattc agggcgtggt gaacaccttc
    10201 gttatcttta tttcccgtat tctggcgcag cttgccgcgg gttttatggg cggaaatcgt
    10261 gatgaaggtg aagagagcaa cggcaacccg ctgatctact ttgcggttgc aacggttctg
    10321 gaactggtgt ttggtattct ggcgagcatt atcaccatgt ggttctcgcg tcatcgtgaa
    10381 ttccatgctg atgccggttc ggcaaaactg gttggtcgcg agaaaatgat tgccgcgctg
    10441 cagcgcctga aaaccagcta tgaaccgcaa gaagcaacca gcatgatggc tttctgcatt
    10501 aatggtaagt cgaaatcgct cagtgagttg ttcatgactc acccgccgct ggataaacga
    10561 attgaagctc tgcgtacggg tgaatacctg aagtaatatg aagtaaatac taaaaagtgc
    10621 gtcctgatcg
//
LOCUS       DADLFA010000142        10506 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000142 DADLFA010000000
VERSION     DADLFA010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10506
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.143"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(72..1100)
                     /locus_tag="JQH65_003084"
     CDS             complement(72..1100)
                     /locus_tag="JQH65_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mal regulon transcriptional regulator MalI"
                     /protein_id="HAY6140417.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1275..2867
                     /locus_tag="JQH65_003085"
     CDS             1275..2867
                     /locus_tag="JQH65_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HAY6140418.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQTQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPAAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYAIFRFAITRFNLKTPGRDSEVASSIEKAVAAAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            2877..4049
                     /gene="malY"
                     /locus_tag="JQH65_003086"
     CDS             2877..4049
                     /gene="malY"
                     /locus_tag="JQH65_003086"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HAY6140419.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDSQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCNMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRIYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4152..5153
                     /gene="add"
                     /locus_tag="JQH65_003087"
     CDS             4152..5153
                     /gene="add"
                     /locus_tag="JQH65_003087"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAY6140420.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNLARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRAGIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5188..6228)
                     /locus_tag="JQH65_003088"
     CDS             complement(5188..6228)
                     /locus_tag="JQH65_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY6140421.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            6441..6566
                     /gene="blr"
                     /locus_tag="JQH65_003089"
     CDS             6441..6566
                     /gene="blr"
                     /locus_tag="JQH65_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HAY6140422.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            6839..7054
                     /gene="ydgT"
                     /locus_tag="JQH65_003090"
     CDS             6839..7054
                     /gene="ydgT"
                     /locus_tag="JQH65_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HAY6140423.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7140..7580
                     /locus_tag="JQH65_003091"
     CDS             7140..7580
                     /locus_tag="JQH65_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HAY6140424.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVSYFKRSSRVKATFVNP"
     gene            7657..8238
                     /gene="rsxA"
                     /locus_tag="JQH65_003092"
     CDS             7657..8238
                     /gene="rsxA"
                     /locus_tag="JQH65_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HAY6140425.1"
                     /translation="MTDYLLLFVGTILVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFTAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            8238..8816
                     /gene="rsxB"
                     /locus_tag="JQH65_003093"
     CDS             8238..8816
                     /gene="rsxB"
                     /locus_tag="JQH65_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HAY6140426.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            8809..>10506
                     /gene="rsxC"
                     /locus_tag="JQH65_003094"
     CDS             8809..>10506
                     /gene="rsxC"
                     /locus_tag="JQH65_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HAY6140427.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDARFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     "
BASE COUNT         2400 a         2723 c         2662 g         2721 t
ORIGIN      
        1 cctgtgtgcc acatttttta tcattaacct tgaggaaagg acactaccag gtatagcaga
       61 taaagagagg attatttcgc tgcaatgagc cgggcgggaa taataagatt gcgtgaatgc
      121 gtctcttcat gggtgatttt ttgcatcatg cgatccgcaa gtgtggtacc aagttcccgc
      181 gcaggcgtgc tggcccaggt aacgggtata tcatcaagtg tggttggtgt cgcatcggta
      241 aatgccgcca gcgaaacttg ttgctcaaag taacgatcga ctccgctttc cccgctttgc
      301 cgccctgctt tcagcaaacc aaaccatgcc cccatcgcaa tagtttcgtt atagcaaacc
      361 acagcactga tggtcgggtt atgacgtaaa agcgccgtga tagcttccgc agcttgcttc
      421 tggctggatg tgcactccag cacccaatcg ctgtgaaacg gcagaccaaa ttttagtaga
      481 gttgcacaat agcctcccac ccgttctgcc cgggttaatg aggaactttg ccctcccagc
      541 caggcgatcc gctgatgccc attgcgaatg agatgctccg tcaacaactg tgcagcctgc
      601 atgttgtccg ggcgaaccgt atcaacatca tcaagataac tggcacggga agcgaaaatc
      661 accgggatag ctttttcttc tgccatccgt cgcaggtcat cgctgcttcc tgcagccccg
      721 gcaattacca caccatcgac cccctgattc agtaacagtg aaaaccgctg tgccagctgc
      781 tcgccgtctt taccgccgtg aagcaaaaaa accatccgtc cctgcgcttc cagagcttcc
      841 gtcaatccgg ccgtcaattc ggcgtaaaac ggcgcagata aatcacggac gatcaaacca
      901 atgacgccgc tttgcccgcc gcgcagcgcc gacgcctggc gattgcgcac aaatcccagc
      961 tcttcaatgg cggcgttaac gcgttctcct gtggcggtag agattcgccc tttgccacta
     1021 agcaccagcg aaacggtact taccgacacg cccgcagcca gcgcaacatc atgaatggtt
     1081 atttttttgg cggtagccat gcagatgacc tactccctga ttatgtgaca gataaaacgt
     1141 tttacctttt atttatctta tacccgctat tatcgttgcg taatgtgatt tatgcctcac
     1201 taaaatttga taaaacgttt tatcttctcg cgcaatttac tgaatccaga ttgttctcta
     1261 cgaggagtcg ttttatgacg gcgaaaacag caccgaaagt cacgctgtgg gagttcttcc
     1321 agcagttagg caaaaccttc atgttacccg tggcattatt gtcgttctgc ggcattatgc
     1381 tcggcattgg tagttctctt agcagccatg atgtcataac cctgatcccg gtcctgggca
     1441 accccgtgtt gcaggctatc tttacctgga tgagtaagat tggctcgttt gcttttagtt
     1501 tcctgcctgt catgttctgt atcgccatcc cgctgggcct ggcacgcgaa aataaaggcg
     1561 tagcggcatt cgctggcttc gtcggttatg cggtaatgaa cctcgcggta aacttctggt
     1621 tgaccaataa aggcattctg ccaaccacgg atgccgcggt tctgaaagcc aataacatcc
     1681 agagcattct tgggatccag tcgatcgata ccgggatcct cggtgcggtg atcgccggta
     1741 ttatcgtctg gatgctgcat gagcgtttcc ataatatccg cctgccggat gcgctggcat
     1801 tcttcggcgg tacgcgcttc gtaccaatta tctcctcgct ggtgatgggc cttgtcggcc
     1861 tggtgattcc attagtctgg ccgattttcg ccatgggtat tagcggcttg ggccatatga
     1921 taaacagcgc gggtgatttc ggaccgatgc tgtttggtac cggtgaacgt ctgctgttgc
     1981 cgtttggtct gcatcacatt ctggtggcat taattcgctt taccgacgca ggcggcacgc
     2041 aggaagtctg cggtcaaacc gtcagcggcg cactgaccat cttccagacg caattgagtt
     2101 gcccgaccac tcacggtttt tctgaaagcg ccacgcgttt cctttcgcaa ggtaaaatgc
     2161 ctgcgtttct cggcggtctg ccagctgcag cgttagctat gtatcactgc gcgcgcccgg
     2221 aaaatcgcca taaaattaaa ggtctgctga tttctggcct gatcgcctgc gtcgttggcg
     2281 gcactaccga accgctggaa ttcctgttcc tgttcgtagc gccagttctg tatgtcatcc
     2341 acgcgctgtt aaccggcctc ggcttcaccg tcatgtctgt gctcggcgtc accatcggta
     2401 ataccgacgg caatatcatc gacttcgtgg tgttcggtat tttgcatggt ctgtcaacca
     2461 agtggtacat ggtgccagtg gtggcggcaa tctggtttgt cgtttactac gccatcttcc
     2521 gtttcgctat cacccgcttc aatctcaaaa ccccggggcg cgatagcgaa gttgccagct
     2581 caatcgaaaa agccgttgcc gctgcgccgg gtaaatcagg ttacaacgtt cctgcaatcc
     2641 tcgaagcatt aggcggtgcc gacaatattg tcagcctcga taactgcatt acccgtctgc
     2701 gtttgtctgt gaaagatatg tcgcttgtta atgtgcaggc actgaaggac aatcgggcaa
     2761 ttggcgtagt acaacttaat caacataacc tgcaggttgt tatcgggcca caagttcagt
     2821 cagtaaaaga tgaaatggcc ggtctgatgc atactgtcca ggcataagga taagatatgt
     2881 tcgatttttc aaaggtcgtg gatcgtcatg gcacatggtg tacacagtgg gattatgtcg
     2941 ctgaccgttt cggcactgct gacctgttac cgttcacgat ttcagacatg gattttgcca
     3001 ctgccccctg cattatagag gcgctgaatc agcgcctgat gcacggcgta tttggctaca
     3061 gccgctggaa aaacgatgag tttctcgctg ctattgccca ctggttttcc acccagcatt
     3121 acaccgccat cgattctcag acggtggtgt atggcccttc tgtcatctat atggtttcag
     3181 aactgattcg tcagtggtct gaaacaggtg aaggcgtggt gatccacaca cccgcctatg
     3241 acgcatttta caaggccatt gaaggtaacc agcgcacagt aatgcccgtt gctttagaga
     3301 agcaggctga tggttggttt tgcaatatgg gcaagttgga agccgtgttg gcgaaaccag
     3361 aatgtaaaat tatgctcctg tgtagcccac agaatcctac cgggaaagtg tggacgtgcg
     3421 atgagctgga gatcatggct gacctgtgcg agcgtcatgg tgtgcgggtt atttccgatg
     3481 aaatccatat ggatatggtt tggggcgagc agccgcatat tccctggagt aatgtggctc
     3541 gcggagactg ggcgttgcta acgtcgggct cgaaaagttt caatattccc gccctgaccg
     3601 gtgcttacgg gattatagaa aatagcagta gccgcgatgc ctatttatcg gcactgaaag
     3661 gccgtgatgg gctttcttcc ccttcggtac tggcgttaac tgcccatatc gccgcctatc
     3721 agcaaggcgc gccgtggctg gatgccttac gcatctatct gaaagataac ctgacgtata
     3781 tcgcagataa aatgaacgcc gcgtttcctg aactcaactg gcagatccca caatccactt
     3841 atctggcatg gcttgattta cgtccgttga atattgacga caacgcgttg caaaaagcac
     3901 ttatcgaaca agaaaaagtc gcgatcatgc cggggtatac ctacggtgaa gaaggtcgtg
     3961 gttttgtccg tctcaatgcc ggctgcccac gttcgaaact ggaaaaaggt gtggctggat
     4021 taattaacgc catccgcgct gttcgttaac cccaattgcg caacgtaaaa atcgttgcgc
     4081 aatcgtggat ttttaccctg ctttgttttt ataatggtgc gcacttttat atccagaaaa
     4141 agagtgcgac catgattgat accaccctgc cattaactga tatccatcgc caccttgatg
     4201 gcaacattcg tccccagacc attcttgaac ttggccgcca gtataatatc tcgcttcctg
     4261 cacaatccct ggaaacactg attccccacg ttcaggtcat tgccaacgaa cccgatctgg
     4321 tgagctttct gaccaaactt gactggggcg ttaaagttct cgcctctctt gatgcctgcc
     4381 gccgcgtggc atttgaaaac attgaagatg cagcccgtaa cggcctgcac tatgtcgagc
     4441 tgcgtttttc accaggctac atggcaatgg cacataagct gcctgtagcg ggtgttgtcg
     4501 aagcggtgat cgatggcgta cgtgaaggtt gccgcacttt tggtgtgcag gcgaagctta
     4561 tcggcattat gagccgaacc ttcggcgaag ccgcctgtca gcaagagctg gaggcctttt
     4621 tagcccaccg tgaccagatt accgcccttg atttagccgg tgatgaactt ggtttcccgg
     4681 gaagtctgtt cctttctcac ttcaaccttg cgcgtgatgc gggctggcat attaccgtcc
     4741 atgcaggcga agctgccggg ccggaaagca tctggcaggc gattcgtgaa ctgggtgcgg
     4801 agcgtattgg acatggcgta aaagccattg aagatcgggc gctgatggat tttctcgccg
     4861 agcaacaaat tggtattgaa tcctgtctga cctccaatat tcagaccagc accgtagcag
     4921 agctggctgc acatccgctg aaaacgttcc ttgagcatgg cattcgtgcc ggcattaaca
     4981 ctgacgatcc cggcgtacag ggagtggata tcattcacga atataccgtt gccgcgccag
     5041 ctgctgggtt atcccgcgag caaatccgcc aagcacagat taatggtctg gaaatggctt
     5101 tcctcagcgc tgaggaaaaa cgcgcactgc gagaaaaagt cgccgcgaag taacaaaatg
     5161 gatggtgcaa attgcaccat ccatttttca tgcaaggcac aaagtcgcgc gatgtttggc
     5221 ggattcgatg cccagctcaa tcaactccat tacctggatt gcctggcttg ccggaaccgg
     5281 attttcacca tcgccattta acgcatcacg aatagccgca tagtaagccg gataattccc
     5341 aggtaccgtc aacagcgttt cttcgacacg ttcctcacct tccactcggg taagtacgcc
     5401 atcacgcata tcgtagcccc agtcttcctg cggcagacgc tcgccatttt tcagacgttc
     5461 ttcctgtgga tcgaggccat atttcacata actgcctcgg gatccatgca cgatataccg
     5521 tgctgactca gcggctgcca gcatggtacc gtgtaaaatg actcgccgct gtggatagga
     5581 caagatggcg tggaaataat cggtcgactg cgctccgggc cgtaactgtg ccaaatctac
     5641 cgtcatgctg accggtaaac caaatagcgt aatggcctga tcaagaagat gcggtgctaa
     5701 atcgtaccag ataccgctgc ctgggccgcc ctgttcacgc caacgatcgc gcacctgcgg
     5761 acggaagcgg tcaaaatgag actcaaagta agcaacttca cccagcacgc cttccgcgag
     5821 caaacctttt agcgtcaaga aatcgctatc ccaacgacgg ttatggaata cagacagcac
     5881 acgccccagg ctttttgcca gcgcatccag ctctcgcgct tgtgacagtg tcacggtaaa
     5941 gggtttatca acgaccacat gtttacccgc ctcaagcgcc gctttggcta acgggaaatg
     6001 ggtatcgttg ggtgtaggaa tgacaatcag gtctatgttg ggatcgttaa acagatgctt
     6061 cggctcagag acaaccgtaa ccgttggcca gtcggctttt acttttgttt catcactgct
     6121 ggagattacc gccagttcca gcccgggcgt gcccgcaatc aggggcgcat ggaaggtttt
     6181 gctcgcataa ccatacccaa tcaacccaac acggatgttg tcgctcatgt cattgcctct
     6241 cattgaagta tgattgctat ttgacactat cctttaccca cgctcaacag tttaatatta
     6301 agtcaaaaaa atagcgaatt ttccaacgac aaaagctaaa tatcgcaaaa acctcagtaa
     6361 aaatcttgct ggagctatta ttgctaagta acatttaccc cctgaagtta atggatcaat
     6421 caagagagat gtgggctgta atgaatcgtc ttattgaatt aacaggttgg atcgttcttg
     6481 tcgtttcagt cattcttctt ggcgtggcga gtcacattga caactatcag ccacctgaac
     6541 agagtgcttc tgtacaacac aagtaagctc tgcgcttgta gagcgccatg ctgcccgtcc
     6601 ggttgcatgt tttcacttgt cggatattaa accaggaatt tattatcttg ttcgatgttg
     6661 ttggtgattg tcagggatag taaagcgcgt tactcttttc agagcagttt ttcatccctg
     6721 aatatttgcc atcagcgcat tcttattatt gtaataacac aagcgttcac ttttggtgaa
     6781 attttatcat cactaataat acctacccct ttggtattaa ttattttatg gaccttttat
     6841 gactgttcag gactacttat taaaatttcg caaaatcagt tcactcgaaa gtctggaaaa
     6901 actctacgac catcttaatt acaccctgac ggacgatcag gaactgatca atatgtatcg
     6961 tgctgccgat caccgtcgcg cagagctggt ttctggcggg cgtttgtttg acctcggcca
     7021 ggtaccgaag tccgtctggc actatgtcca ataaagaaag tagcgatcgg ttataaaagc
     7081 cttataataa cgcccctaaa attagatttg cctgtttgcg tcgctattct ggagaaccta
     7141 tgaccaccac aacgccacaa cgtattggag gctggttgct tggcccgttg gcctggcttt
     7201 tagtcgctct gttgagtacg acgctggcgt tgttgctgta taccgctgcg ttatcttctc
     7261 cgcaaacatt tcaaacgctc ggcggacaag cactgacgac acaaatctta tggggcgttt
     7321 cttttattac cgctatcgcc atgtggtatt acacactgtg gttgaccatc gctttcttta
     7381 aacgtcggcg ttgtgttcct aaacactata ttatctggct gcttatttcc gtattgctgg
     7441 cagtaaaagc attcgctttt tcacccgtgg aagatggcat agccgttcgc cagttgctgt
     7501 ttactttgtt ggcaaccgca ttgatcgtct cctatttcaa acgctcgtcg cgggtaaaag
     7561 cgacgtttgt gaatccgtaa taaccttaca gttaacctgt tgtcgcctgc tctggattaa
     7621 cggataatag gcggcttttt tatttcaggc cgaaaaatga ctgactacct gttactgttt
     7681 gtcggaacta tactggtcaa taactttgtg ctggtcaagt ttctcggtct ctgtccgttt
     7741 atgggggttt ccaaaaaact ggaaaccgcg atgggcatgg ggctggcaac aacgtttgtg
     7801 atgacgctgg cgtctatttg cgcctggctt atcgatacgt ggattttgat cccacttaat
     7861 ctgatttacc tgcgcaccct ggcatttatt ctggtgattg ctgtggtcgt gcagttcacc
     7921 gagatggtgg tgcgcaaaac cagcccggtg ctttaccgtc tgctggggat ttttttgccg
     7981 cttatcacca ccaactgtgc agtgctcggc gtggcgttgc tgaatatcaa tctcgggcac
     8041 aatttcttgc agtcggcgct gtacggtttt tccgccgctg tcggtttctc gctggtgatg
     8101 gtgctcttca ccgccatccg cgaacgcctt gctgtggctg atgtcccggc accttttcgc
     8161 ggtaatgcca ttgcgttaat taccgcaggt cttatgtctc tggcctttat gggctttagt
     8221 ggtttggtga agttgtaatg aatgctatct ggattgccgt tgccgccgtg agcctgctgg
     8281 gcctggcgtt tggcgccatt ctgggttatg cctcccgccg ttttgcggtg gaagacgatc
     8341 cggtcgttga gaaaattgac gaaatcttac cgcagagcca gtgtggtcag tgcggttatc
     8401 ccggctgtcg cccctacgcg gaagccatca gctgtaacgg tgaaaaaatc aaccgttgcg
     8461 ccccaggtgg cgaagctgtg atgctaaaaa ttgccgagtt gcttaatgtc gagccgcagc
     8521 cgctggatgg cgaagcgcaa gagctaacgc ctgcgcggat ggtggcggtt attgatgaaa
     8581 ataactgtat tggctgcact aaatgtattc aggcttgtcc ggtagacgcc atcgttggcg
     8641 ctacccgtgc catgcatacg gtaatgagtg atctctgtac gggctgcaat ttatgtgttg
     8701 atccgtgccc gacgcactgc atctcgttgc aaccggtcgc agaaacacct gactcctgga
     8761 aatgggatct gaacaccatt cccgtgcgta tcattcccgt ggaacaccat gcttaagtta
     8821 ttctctgcat tcagaaaaaa taaaatctgg gatttcaacg gcggcatcca tccaccggag
     8881 atgaaaaccc agtccaacgg tacccccctg cgccaggtac cactggcgca gcgttttgtt
     8941 attccactga aacagcatat tggcgctgaa ggtgagttgt gcgttagcgt cggcgataaa
     9001 gtattgcgcg gccagccgct tacccgtggt cgcggcaaaa tgctgcctgt tcacgcgccc
     9061 acctcgggta ccgttacggc tattgcgccc cactctacgg ctcatccttc agctttagct
     9121 gaattaagcg tgattattga tgccgatggt gaagactgct ggatcccgcg cgacggctgg
     9181 gccgattatc gcactcgtag tcgcgaagag ttaatcgagc gcatacatca gtttggtgtt
     9241 gccgggctgg gcggtgcagg attcccgaca ggcgttaaat tgcagggtgg cggagataag
     9301 attgaaacgt tgattatcaa cgcggctgag tgcgagccgt acattaccgc cgatgaccgt
     9361 ttgatgcagg attgcgcggc tcaggtcgta gagggtattc gcattcttgc gcatattctg
     9421 cagccacgcg aaattcttat cggcattgaa gataacaaac cgcaggcgat ttccatgctg
     9481 cgcgcggtgc tggcggactc taacgatatt tctctgcggg tgattccaac caaatatcct
     9541 tctggcggtg ctaaacaatt aacctacatt ctgaccggga agcaggttcc acatggcggg
     9601 cgttcatccg atatcggcgt attaatgcaa aacgtcggca ctgcttatgc agtgaaacgt
     9661 gccgttattg atggcgagcc gattaccgag cgtgttgtaa ccctgactgg cgaagcaatc
     9721 gctcgcccgg gcaacgtctg ggcacggctg gggacgccag tgcgtcattt attgaatgat
     9781 gcccgattct gcccctctgc cgatcaaatg gtgattatgg gtggcccgct aatgggcttt
     9841 accttgccat ggctggatgt cccggtcgta aagattacca actgtctgtt ggctccctct
     9901 gccaatgaac ttggcgaacc acaggaagaa caaagctgca tccggtgtag cgcctgtgct
     9961 gacgcctgcc ctgcggatct tttgccgcaa cagttgtact ggttcagcaa aggtcagcaa
    10021 cacgataaag ctaccacgca taacattgct gattgcattg aatgtggggc ttgcgcgtgg
    10081 gtttgcccga gcaatattcc cctggtgcaa tatttccgtc aggaaaaagc tgaaattgcg
    10141 gctattcgtc aggaagaaaa gcgcgccgca gaagccaaag cgcgtttcga agcgcgccag
    10201 gctcgtctgg agcgcgaaaa agcggctcgc cttgaacgac ataagagcgc agccgttcaa
    10261 cctgcagcca aagataaaga tgcgattgct gccgctctgg cgcgggtgaa agagaaacag
    10321 gcccaggcta cacagcctat tgtgattaaa gcgggcgaac gcccggataa cagtgcaatt
    10381 attgcagcac gggaagcccg taaagcgcaa gccagagcga aacaggcaga actgcagcaa
    10441 actaacgacg cagcaaccgt tgctgatcca cgtaaaactg ccgttgaagc agctatcgcc
    10501 cgcgcc
//
LOCUS       DADLFA010000143        10463 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000143 DADLFA010000000
VERSION     DADLFA010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10463
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.144"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(28..1926)
                     /locus_tag="JQH65_003095"
                     /pseudo
     CDS             complement(28..1926)
                     /locus_tag="JQH65_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            2448..4376
                     /gene="thrS"
                     /locus_tag="JQH65_003096"
     CDS             2448..4376
                     /gene="thrS"
                     /locus_tag="JQH65_003096"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAY6140428.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHAARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            4380..4922
                     /gene="infC"
                     /locus_tag="JQH65_003097"
     CDS             4380..4922
                     /gene="infC"
                     /locus_tag="JQH65_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAY6140429.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQRVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            5019..5216
                     /gene="rpmI"
                     /locus_tag="JQH65_003098"
     CDS             5019..5216
                     /gene="rpmI"
                     /locus_tag="JQH65_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAY6140430.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            5269..5625
                     /gene="rplT"
                     /locus_tag="JQH65_003099"
     CDS             5269..5625
                     /gene="rplT"
                     /locus_tag="JQH65_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016169860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAY6140431.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     gene            5748..5792
                     /locus_tag="JQH65_003100"
     CDS             5748..5792
                     /locus_tag="JQH65_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl--tRNA ligase operon leader peptide"
                     /protein_id="HAY6140432.1"
                     /translation="MNAAIFRFFFYFST"
     gene            6075..7058
                     /gene="pheS"
                     /locus_tag="JQH65_003101"
     CDS             6075..7058
                     /gene="pheS"
                     /locus_tag="JQH65_003101"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAY6140433.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            7073..9460
                     /gene="pheT"
                     /locus_tag="JQH65_003102"
     CDS             7073..9460
                     /gene="pheT"
                     /locus_tag="JQH65_003102"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012905611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAY6140434.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDCLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            9465..9764
                     /gene="ihfA"
                     /locus_tag="JQH65_003103"
     CDS             9465..9764
                     /gene="ihfA"
                     /locus_tag="JQH65_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HAY6140435.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            complement(10067..10207)
                     /locus_tag="JQH65_003104"
     CDS             complement(10067..10207)
                     /locus_tag="JQH65_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HAY6140436.1"
                     /translation="MSQKSLTAITFCVTAILIIWMLHGSLCEIRMSFWGAEFAAFLQC
                     KQ"
BASE COUNT         2751 a         2368 c         2666 g         2678 t
ORIGIN      
        1 taagttggca gcattaccaa tagttggtta taaatacgtc tttagttcat cagcactaaa
       61 actcaggctt tggctgatta catttatagc agcataatat gtttctacag ttttataatg
      121 attatgatga atggctatat gaacagctga catgttgtca tgatttttag cggccaacag
      181 tgtgaataac tgacgttgac taaaagaata tttttttgca aaaatattca gcgtcgatat
      241 ataagataac acgacgtctt cattaccctt gctcatggcg atgtataata caggggttcc
      301 atgtgctgta gcgcctttta ataaatccat gatgttagct ttgctcaact tgtatttaaa
      361 ggcgatgcca ttaattttag agaggaaccg tgtgacacac aaagggtgat tattttccat
      421 tgcagcaaac aaccctagtt caccatcacg tgttttcatt gctaaaatat ccatcactat
      481 ttttttgttt aaaagctctt gtgctattgg agtggttaat gcattcagaa agatatcgat
      541 tgtttcatcg aacccataca acatggagat atagagccca ggtgtttcgt tagcactttt
      601 agtttgcagt aatttaacca gattatcttc atgaatgata tgattgtata tcagtgactg
      661 aatttcttgc ataaatattt ttacgttgtc agtatgtccg ttcatcatag ccagaaataa
      721 agcaggtgta ccaacgctat tctgcgcagt tattatttca atgatttctt tttcagggag
      781 tatgggcaac tgtttgaata atgtaataag ctgttgagtc tggcctgtgg ccattattaa
      841 caacattgcc gatggatgta taggggcaaa tggcattcgt gatgcaatac ccgcgaaaat
      901 agcccctgga actcccacag gtaatagggg cagaatagca attgcacagt tgcgaacata
      961 cctatcagcc caggtctgag catcgggacc tgaactgatg aacttatcaa gcgaaagaaa
     1021 gccccgtgct gtcctgtggg tgcctctgac ggcaacgttg gtatcgttcg ggtcgtagac
     1081 ggacactaca tagtttaatt gtccatacac taactgcttt attcgtagcc ttactgacaa
     1141 agtatgattg tctatatata aaatcgcgga catatgactg acattgtttg ccatcattcg
     1201 acaaaagaaa atatctaaag cgaagccaaa atccttactc gcgataacgc gactccctgc
     1261 aggaggatgg cgaagtaatg ctctgaactc cgcatcccgg actaagggga catgctgctg
     1321 tatggattca acggaggagt aatgtttaat caggttgact ttgccttttc catttctcaa
     1381 tgaatcaagg gcgtattgca atgacagatg gcgacaaaaa atagcgtcag aaggagattg
     1441 caaaaaacat gcactaccat tgagatttac tgttggttgt atagtttttt ctctatggat
     1501 ttttttagac agtttcatac aatggttacg atcttcggca gttaactgtt cctgctcaat
     1561 ataatccagt atgcttcaca aatggttagc cttaataggc gtggtttcca gaaataaggg
     1621 aatgatttct tttttatagt gtaatgtaat aagtatattt tctgcatgga tgggtgaagt
     1681 ggcaaagtta atgataaaaa gggcagtgga gtaacaattt tgtgatattt ttcgacagtt
     1741 tgcggtaaat tcctgagtaa tacagtatga cagcatcgaa cttaagtcag aggtatcaag
     1801 tagggtgatg agttggcgcg caaattgatt aataagctct gatgaaggcg ttggtatgtc
     1861 gctatcgatg tgcaatctat gaggagactt ataatgagtt ctgataatga tatcgttttg
     1921 actcataatc tattcacctt atatatactg gaggttttga ggatgatgat taattattat
     1981 cgccagaacg aaatgtgcac gaaaaattgc tataagaatt gtgtcacttt tatcattgtg
     2041 atatgaccac ttcgtcatta atctcatgat cgtttaaata ctaccgccaa atcacgcaac
     2101 gaactttgct ggattcagtt tatgctgctg taaatccgct cgcagtaaac ctttcagacg
     2161 caccgtgatg ttatcagtgg ttctttaagc gttttgctgg tgtactcact acaaacgaat
     2221 tgcgaatcga atcaatgtga aacggaaagg tacaatctcc ctttttttag tgttaacatc
     2281 gttcaaccgg gtggaaggtg agaggttaat gtctttcatt tgtgtacctg attgcgaacc
     2341 aatttagcat ttgttggcta gatggtttcg caatgaactg ttaataaaca aatttttctt
     2401 tgtatgtgat ctttcgtgtg ggtcaccact gcaaataagg atataaaatg cctgttataa
     2461 ctcttcctga tggcagccaa cgccattacg accacgctgt aagccccatg gatgttgcgc
     2521 tggacattgg tccaggtctg gcgaaagcct gtatcgcagg gcgcgttaat ggcgaactgg
     2581 ttgatgcttg cgatctgatt gaaaacgacg cacaactgtc gatcattacc gccaaagacg
     2641 aagaaggtct ggagatcatt cgtcactcct gtgcgcacct gttagggcat gcgattaaac
     2701 aactttggcc gcataccaaa atggcaatcg gcccggttat tgacaacggt ttttattacg
     2761 acgttgatct tgaccgcacg ttaacccagg aagatgtcga agcactcgag aagcggatgc
     2821 atgagcttgc tgagaaaaac tacgacgtca ttaagaagaa agtcagctgg cacgcagcgc
     2881 gtgaaacttt cgccaaccgt ggggagagct acaaagtctc cattcttgac gaaaacatcg
     2941 cccatgatga caagccaggt ctgtacttcc atgaagaata tgtcgatatg tgccgcggtc
     3001 cgcacgtacc gaacatgcgt ttctgccatc atttcaaact aatgaaaacg gcaggggctt
     3061 actggcgtgg cgacagcaac aacaaaatgt tgcaacgtat ttacggtacg gcgtgggcag
     3121 acaaaaaagc acttaacgct tacctgcagc gcctggaaga agccgcgaaa cgcgaccacc
     3181 gtaaaatcgg taaacagctc gacctgtacc atatgcagga agaagcgccg ggtatggtat
     3241 tctggcacaa cgacggctgg accatcttcc gtgaactgga agtgtttgtt cgttctaaac
     3301 tgaaagagta ccagtatcag gaagttaaag gtccgttcat gatggaccgt gtcctgtggg
     3361 aaaaaaccgg tcactgggac aactacaaag atgcaatgtt caccacatct tctgagaacc
     3421 gtgaatactg cattaagccg atgaactgcc cgggtcacgt acaaattttc aaccaggggc
     3481 tgaagtctta tcgcgatctg ccgctgcgta tggccgagtt tggtagctgc caccgtaacg
     3541 agccgtcagg ttcgctgcat ggcctgatgc gcgtgcgtgg atttacccaa gatgacgcgc
     3601 atatcttctg tactgaagaa caaattcgcg atgaagttaa cggatgtatc cgtttagtct
     3661 atgatatgta cagcactttt ggcttcgaga agatcgtcgt caaactctcc actcgtcctg
     3721 aaaaacgtat tggcagcgac gaaatgtggg atcgtgctga ggcggacctg gcggttgcgc
     3781 tggaagaaaa caacatcccg tttgaatatc aactgggtga aggcgctttc tacggtccga
     3841 aaattgaatt taccctgtat gactgcctcg atcgtgcatg gcagtgcggt acagtacagc
     3901 tggacttctc cttgccgtct cgtctgagcg cctcctatgt gggcgaagac aacgagcgta
     3961 aggtaccggt aatgattcac cgcgcaattc tggggtcgat ggaacgtttc atcggtatcc
     4021 tgaccgaaga attcgctggt ttcttcccga cctggcttgc gccagttcag gttgttatca
     4081 tgaatattac cgattcacag tctgaatacg ttaacgaatt gacgcaaaaa ctatcaaatg
     4141 cgggcattcg tgttaaagca gacttgagaa atgagaagat tggctttaaa atccgcgagc
     4201 acactttgcg tcgcgtccca tatatgctgg tctgtggtga taaagaggtg gaatcaggca
     4261 aagttgccgt tcgcacccgc cgtggtaaag acctgggaag catggacgta aatgaagtga
     4321 tcgagaagtt gcaacaagag attcgcagcc gcagtcttaa acaattggag gaataaggta
     4381 ttaaaggcgg aaaacgagtt caaacggcgc gccctaaccg tatcaatggc gaaattcgcg
     4441 cccaggaagt tcgcttaaca ggtctggaag gcgagcagct tggtattgtg agtctgagag
     4501 aagctctgga gaaagcagaa gaagccggag tagacttagt cgagatcagc cctaacgccg
     4561 agccgccggt ttgtcgtata atggattacg gcaaattcct ctatgaaaag agcaagtctt
     4621 ctaaggaaca gaagaaaaag caaagagtta tccaggttaa ggaaattaaa ttccgtcctg
     4681 gtacagatga aggcgactat caggtaaaac tccgcagcct gattcgcttt ctcgaagagg
     4741 gtgataaagc caaaatcacg ctgcgtttcc gcggtcgtga gatggcgcac cagcaaatcg
     4801 gtatggaagt gcttaatcgc gtgaaagacg atttgcaaga actggcagtg gtcgaatcct
     4861 tcccaacgaa gatcgaaggc cgccagatga tcatggtgct cgctcctaag aagaaacagt
     4921 aaggccttca agtagcactg tctgtggagc cttcgggctt cacaggcttt gttcgccttt
     4981 gtttcgttta ttaacaatgc gaagtggaag ttattaaaat gccaaaaatt aagaccgtac
     5041 gcggtgctgc taagcgcttc aaaaaaaccg gtaaaggtgg ttttaagcac aagcacgcta
     5101 acctgcgtca cattctgacc aaaaaagcga ccaaacgtaa acgtcacctg cgtccgaaag
     5161 ccatggtttc caaaggcgat ctgggcctgg taatcgcgtg cctgccgtac gcataagccg
     5221 ttaacgtttt taacttttta attagaatat agatacagga gagcacatat ggctcgcgta
     5281 aaacgtggtg ttattgcacg cgcacgtcac aagaaaattt tgaaacaagc taaaggctac
     5341 tacggtgcgc gttctcgcgt ataccgcgtt gccttccagg ctgttatcaa agctggtcag
     5401 tatgcttacc gtgaccgtcg tcaacgtaag cgtcagttcc gtcaactgtg gattgcacgt
     5461 atcaacgcag cagcacgtca gaacggtatt tcttacagca aattcatcaa tggcctgaaa
     5521 aaagcctctg ttgaaatcga ccgtaagatc ctggctgata tcgcagtatt cgacaaagta
     5581 gcgttcaccg ctctggttga aaaagcgaaa gcagctctgg cataagccag ttgaaagagg
     5641 gagctagtct ccctcttttc gtttcaacgc catcaaaaca ttgactttta tcgccgtagc
     5701 cttttcaata aaggtctttt gaagagtacc aaaaggtaac gcaagcaatg aatgctgcta
     5761 ttttccgctt ctttttttac tttagcacct gaatccagga ggctagcgcg tgagaagaga
     5821 aacggaaaac agcgcctgaa agcctcccag tggaggcttt ttttgtatgc gcgttttgaa
     5881 aaataaaaag ccagtctatc aggcgtaatt gtcgcagcca gtctggatac ggccagcgcg
     5941 gagaaaccgg agcgtattta agtacgtgag aatttcgagc actgcccggg accaaaatgg
     6001 caagtaaaat agcctgatgg gataggctct aagtccaacg aaccagtgtc accactgaca
     6061 caatgaggaa aaccatgtca catctcgcag aactggttgc cagtgcgaag gcggccatta
     6121 gccaggcgtc agatgttgcc gcgttagata atgtgcgcgt cgaatatttg ggtaaaaaag
     6181 ggcacttaac ccttcagatg acgaccctgc gtgagctgcc gccagaagag cgtccggcag
     6241 ctggtgcggt tatcaacgaa gcgaaagagc aggttcagca ggcgctgaat gcgcgtaaag
     6301 cggaactgga aagcgcagcg ctgaatgcgc gtctggcggc ggaaacgatt gatgtctccc
     6361 tgccaggtcg tcgcattgaa aacggcggtc tgcatccggt tacccgtacc atcgaccgta
     6421 tcgaaagttt cttcggtgag cttggcttta ccgtggcaac cgggccggaa atcgaagacg
     6481 attatcataa cttcgatgct ctgaacattc ctggtcacca cccggcgcgc gctgaccacg
     6541 acactttctg gtttgacact acccgcctgc tgcgtaccca gacctctggc gtacagatcc
     6601 gcaccatgaa agcccagcag ccaccgattc gtatcatcgc gcctggccgc gtttatcgta
     6661 acgactacga ccagactcac acgccgatgt tccatcagat ggaaggtctg attgttgata
     6721 ccaacatcag ctttaccaac ctgaaaggca ctctgcacga tttcctgcgt aacttctttg
     6781 aggaagattt gcagattcgc ttccgtcctt cctacttccc gttcaccgag ccttctgcag
     6841 aagttgacgt catgggtaaa aacggtaaat ggctggaagt gctgggttgc gggatggtgc
     6901 atccgaacgt gctgcgtaac gttggcatcg acccggaagt ttactctggt ttcgccttcg
     6961 ggatggggat ggagcgtctg actatgttgc gttacggcgt caccgacctg cgttcattct
     7021 tcgaaaacga tctgcgtttc ctcaaacagt ttaaataagg caggaataga ttatgaaatt
     7081 cagtgaactg tggttacgcg aatgggtgaa cccggcgatt gatagcgatg cgctggcgaa
     7141 tcaaatcact atggcgggcc tggaagttga cggtgtagaa ccggttgccg gtagcttcca
     7201 cggcgtggtc gttggtgaag tggttgagtg tgcgcagcat ccgaacgctg acaaactgcg
     7261 tgtgacaaaa gtgaatgtcg gcggcgatcg cctgctggac atcgtctgtg gtgcgccaaa
     7321 ctgccgtcag ggcctgcgtg tggcggtagc taccattggt gctgttctgc cgggtgattt
     7381 caaaattaaa gcggcgaaac tgcgtggcga accgtctgaa gggatgctgt gctccttctc
     7441 tgaactggga atttctgacg atcacagcgg cattatcgaa ctgcctgcag atgcgccgat
     7501 tggcactgac atccgcgaat acctgaaact cgatgacaac accatcgaaa tcagcgtaac
     7561 gccaaaccgt gccgactgct taggtatcat tggtgttgcg cgtgacgttg ccgtgctgaa
     7621 tcagctgccg ctggttgaac cggaaatcgt tccggttggt gcgaccatcg acgacacgtt
     7681 gccgattaca gtcgaagcgc ctgaagcctg cccgcgctat cttggccgtg tggtaaaagg
     7741 cattaacgtt aaagcgccaa ctccgctgtg gatgaaagaa aaactgcgtc gttgcgggat
     7801 ccgttctatc gatgcagttg ttgacgtcac caactatgta ctgctcgaac tgggccagcc
     7861 gatgcacgct ttcgataaag atcgcattga aggcggcatt gtggtgcgga tggcaaaaga
     7921 gggcgaaacg ctggtgctgc ttgatggcac tgaagcgaag ctgaatgctg acactctggt
     7981 catcgccgac cacaacaagg cgctggcgat gggcggcatc tttggtggcg aacactctgg
     8041 cgtgaatgac gaaacgcaaa acgtcttgct ggaatgcgct ttcttcagcc cgctgtctat
     8101 caccggtcgt gctcgtcgtc atggcctgca tactgatgcg tctcaccgtt atgagcgtgg
     8161 cgttgatccg gcactgcagc acaaagcgat ggaacgtgcg acccgtctgc tgatcgacat
     8221 ctgcggtggt gaggctggtc cggtaattga tatcaccaac gaagcaacgc tgccgaagcg
     8281 tgcaaccatc actttacgtc gtagcaaact ggattgcctg atcggccatc atattgctga
     8341 tgaacaggta actgacattc tgcgtcgtct cggctgcgaa gtgaccgaag gcaaagacga
     8401 gtggcaggca gttgcgccga gctggcgttt cgatatggag attgaagaag atctggtcga
     8461 agaagtcgcg cgtgtttacg gctacaacaa catcccggat gagccggtac aggcaagcct
     8521 gattatgggt actcaccgtg aagctgacct gtcgctcaag cgcgtgaaaa cgctgctcaa
     8581 cgacaaaggc tatcaggaag tgatcaccta cagcttcgtt gatccgaaag tgcagcagat
     8641 gatccatcca ggcgttgaag ccttactgct gccaagcccg atctctgttg aaatgtcagc
     8701 aatgcgtctt tctctgtgga ccggcctgct ggcaaccgtg gtgtacaacc agaaccgtca
     8761 gcagaaccgt gtgcgcattt tcgaaagcgg tctgcgtttc gtaccagata ctcaggcacc
     8821 gttgggcatt cgtcaggatc tgatgttagc cggtgtgatt tgcggtaacc gttacgaaga
     8881 gcactggaac ctggcaaaag agaccgttga tttctatgat ttgaaaggcg atcttgaatc
     8941 cgttctcgac ctgaccggta aactgaatga ggttgagttc cgtgcagaag cgaatccggc
     9001 actgcatccg gggcaatccg cagcgattta tctgaaaggg gaacgtattg gttttgttgg
     9061 agttgttcat cctgaactgg aacgtaaact ggatcttaac ggtcgcactc tggtgttcga
     9121 actggagtgg aacaagctcg cagaccgcgt ggtgcctcag gcgcgcgaga tttctcgctt
     9181 cccggcgaac cgtcgtgaca tcgcggtggt ggtcgcagaa aacgttcccg cagcggatat
     9241 tttatccgaa tgtaagaaag ttggcgtaaa tcaggtagtt ggcgtaaact tatttgacgt
     9301 gtaccgcggt aagggtgttg cggaggggta taagagcctc gccataagcc tgatcctgca
     9361 agataccagc cgtacactcg aagaagagga gattgccgct accgtcgcca aatgtgtaga
     9421 ggcattaaaa gagcgattcc aggcatcatt gagggattga acctatggcg cttacaaaag
     9481 ctgaaatgtc agaatatctg tttgataagc ttgggcttag caagcgggat gccaaagaac
     9541 tggttgaact gtttttcgaa gagatccgtc gcgctctgga aaacggcgaa caggtgaaac
     9601 tctctggttt tggtaacttc gatctgcgtg ataagaatca acgcccggga cgtaacccga
     9661 aaacgggcga ggatattccc attacagcac ggcgcgtggt gaccttcaga cccgggcaga
     9721 agttaaaaag ccgggtcgaa aacgcttcgc ccaaagacga gtaatctgat ctaactaaaa
     9781 aggccgctct gcggcctttt ttctttcaat aactaattga tttataatgt tttatatctc
     9841 ttcgttccac gatgcgtcca catttagtgg gggaggggag taagcccgct acattgtggg
     9901 ctttgtctta tagtctgatc actgtttcaa gtcggtgagg acaccaaaaa gaacccccgc
     9961 agtttttacg ctgcgggttt gttgttcatg tctgtgagat agggtgcctt atcgacctta
    10021 ccctggcaac cgattgacgg gggattgctc ccccgtcgcg gtttccttac tgcttacact
    10081 gtaagaacgc cgcaaactcc gctccccaga agctcatccg tatttcgcac agcgaaccgt
    10141 gcagcatcca gatgatgagg attgccgtca cgcagaacgt gatggccgta agcgattttt
    10201 gcgacatagc gcttgctcct ttttcgggga ggcgctaacc tatcacttgc ttagggtaga
    10261 cggttagggc ctcggttaaa caaaaatgtt ttccggggcc tttccacatc tggccttcag
    10321 gtattccctc cgaccatcag ccgaaaggca cccgcgcgta atctatcgct tttttgttac
    10381 tccggcaatt ctgctcgtta attctgaggt aagggcaaac tcatctgatt gtttcctctg
    10441 tgtgaagttg gcagttcatg cca
//
LOCUS       DADLFA010000144        10370 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000144 DADLFA010000000
VERSION     DADLFA010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10370
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.145"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            105..1385
                     /gene="wcaK"
                     /locus_tag="JQH65_003105"
     CDS             105..1385
                     /gene="wcaK"
                     /locus_tag="JQH65_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HAY6140437.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLSEAVSRERQTGMQMVQSVLERIGEVK"
     gene            1382..2602
                     /gene="wcaL"
                     /locus_tag="JQH65_003106"
     CDS             1382..2602
                     /gene="wcaL"
                     /locus_tag="JQH65_003106"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HAY6140438.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTSWLQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLNTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            2613..4007
                     /gene="wcaM"
                     /locus_tag="JQH65_003107"
     CDS             2613..4007
                     /gene="wcaM"
                     /locus_tag="JQH65_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HAY6140439.1"
                     /translation="MPFKKISRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELYNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNEKGQSSVDIDRVNHHIV
                     NVEKINFRLPERRE"
     gene            4182..5075
                     /gene="galF"
                     /locus_tag="JQH65_003108"
     CDS             4182..5075
                     /gene="galF"
                     /locus_tag="JQH65_003108"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HAY6140440.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELEHTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            5448..6533
                     /gene="rfbB"
                     /locus_tag="JQH65_003109"
     CDS             5448..6533
                     /gene="rfbB"
                     /locus_tag="JQH65_003109"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAY6140441.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAVMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRLHHISTDEVYGDLPHPDEVNNTEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYIADRPGHDRRYAIDAEKIGRELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQSWIEENYEGRQ"
     gene            6533..7432
                     /gene="rfbD"
                     /locus_tag="JQH65_003110"
     CDS             6533..7432
                     /gene="rfbD"
                     /locus_tag="JQH65_003110"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAY6140442.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNSE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPEFAQLLNATSVEAIAKAANEVGAWVIHY
                     STDYVFPGTGEIPWQEADATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVPTTAYPTPARRPHNSRLDTEKFQQNFA
                     LVLPDWQIGVKRMLNELFTTTAI"
     gene            7490..8368
                     /gene="rfbA"
                     /locus_tag="JQH65_003111"
     CDS             7490..8368
                     /gene="rfbA"
                     /locus_tag="JQH65_003111"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAY6140443.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPTPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSSRGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            8373..8942
                     /gene="rfbC"
                     /locus_tag="JQH65_003112"
     CDS             8373..8942
                     /gene="rfbC"
                     /locus_tag="JQH65_003112"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAY6140444.1"
                     /translation="MNVIKTEISDVLIFEPKVFGDERGFFFESFNQKVFEEAVGRKVE
                     FVQDNHSKSSKGVLRGLHYQLEPYAQGKLVRCAVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGLAHGFLVLSETAEFLYKTTNYYHPESDRGIIWDDPDIDVKWPLS
                     IHKPILSIKDEKQKMFKEMIALEKWNGEY"
     gene            8929..9771
                     /locus_tag="JQH65_003113"
     CDS             8929..9771
                     /locus_tag="JQH65_003113"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6140445.1"
                     /translation="MENTNYYKLAILIVLYGKEVEQCETFTHIDRVLKKNRMSKDDII
                     LCLWNNGPREIAFNLTTDYYTLKVCQTINNESLSYIYNLFINEVESEKYILLDHDSIL
                     KYDYVLECLNSTSDCIIPNIIVGNNIQSPKTYSIQSFNKGNTVLAIGSGICISKKLCE
                     KLRQVFGDVFDSRFYFYGVDSTFFLRLNKIGESEFIKISECVVLHSLSRLENEAIKVK
                     QFRIKERSYDQGLTLRYYFCKFTFFTVAKKLLAKLIFNKSDISLIYFAKAYFSGKHYK
                     SGKS"
     gene            9768..>10370
                     /locus_tag="JQH65_003114"
     CDS             9768..>10370
                     /locus_tag="JQH65_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAY6140446.1"
                     /translation="MKFIIIGYRDAYFWNEYGTSVRDLQIAEILSRKYHVTFINRPVS
                     IYERLKNKKKAKSEYKAYSNRITFIDHTSYDLIGPLFGRIWTKYCYKKILNRISSLFT
                     DDSIIIDFTPIAELEFKNNKRITYWYDLIDNFSIHNRFSEKEKKAVRDKYAYVDENAD
                     IITAVSDKAIKNFLNPNKYVMPNGVYLENQNHECFIDGTPN"
BASE COUNT         2973 a         2157 c         2499 g         2741 t
ORIGIN      
        1 gggtcttgta ggcctgataa gacgcgccag cgtcgcatca ggcaaaaaac ggcaacgaac
       61 aaaacaaacg cagcctcacc tggcatcaaa tttatgagga aaaaatgaaa ttacttattc
      121 tgggcaacca cacttgcggc aatcgtggcg acagcgccat cttgcgtggc ttacttgatg
      181 ccatcaacat cctcaatcca cacgccgaag tggacgtgat gagccgctat ccggtcagtt
      241 cttcctggct gctcaaccgc ccggtaatgg gcgatccgct gttcctgcaa atgaaacaac
      301 acaacagcgc ggcgggcgtt gtcgggcgcg ttaaaaaagt cctccgtcgc cgctatcaac
      361 accaggtatt gctctcgcgc gtcaccgaca ctggcaagct gcgcaatatt gccatcgccc
      421 agggattcac tgacttcgtg cgcctactgt caggttacga tgccattatc caggtcggcg
      481 gatcgttttt tgtcgatctc tatggcgtgc cgcagtttga acatgcactt tgcacgttta
      541 tggcgaaaaa gccgctgttt atgattggtc acagcgtcgg tcccttccag gatgagcaat
      601 ttaaccaact ggcgaactac gtttttggtc actgtgacgc gctgatcctg cgcgaatcgg
      661 tcagccttga tctgatgaaa cgcagcaata tcaccactgc aaaagtggaa catggcgtcg
      721 atactgcgtg gctggtcgat caccacaccg aagacttcac tgccagctat gccgttcaac
      781 actggctgga cgttgccgca caacagaaaa cggtggcaat taccctgcgc gaactggcac
      841 cgttcgacaa acgtctcggc accactcagc aagcgtatga aaaagccttt gccggggtgg
      901 tcaatcgcat tctcgacgaa ggctatcagg tgattgcgct ctccacctgt acgggcatcg
      961 acagctataa caaagacgac cgcatggtgg cgctcaactt gcgtcagcac atcagcgatc
     1021 ctgcccgtta ccacgtagtg atggatgaac tcaacgatct ggaaatgggc aaaattctgg
     1081 gggcctgtga actcaccgtc ggtacgcgcc tgcactccgc cattatctca atgaactttg
     1141 ctactccggc gattgccatc aactatgaac ataaatccgc cgggattatg cagcagctgg
     1201 gactaccgga gatggcaatt gatatccgtc atttattaga cggcagcctg caagcgatgg
     1261 ttgcggatac cttaggccag cttccggcgc tgaacgcacg acttagcgaa gccgtcagtc
     1321 gtgagcgtca gacgggaatg cagatggtgc aatctgtgct tgagcgcatc ggggaggtga
     1381 aatgaaggtc ggcttctttt tactgaaatt tccgctgtct tcggaaacct tcgtcctcaa
     1441 tcaaattacc gcgtttattg atatgggctt tgaggtggag attgtcgcgc tgcaaaaagg
     1501 cgacacccag aacacccacg cggcatggac gaaatacaac cttgccgcaa gaaccagttg
     1561 gttacaggac gaacctacgg gcaaagtggc gaagctacgc caccgtgcca gccagacgtt
     1621 acgaggcatt catcgtaaaa atacctggca ggcgctcaac ctcaaacgct atggcgcaga
     1681 gtcgcggaat ctgattttgt ctgccatttg cggccaggtc gcaacaccgt ttcgcgccga
     1741 tgtgttcatc gctcattttg gtccagcggg ggtaaccgca gcaaaactcc gcgaactggg
     1801 tgtcattcgc ggcaaaattg ccaccatttt ccacggcatt gatatttcca gtcgggaagt
     1861 gctcaaccac tacactcccg aatatcaaca actgtttcgc cgtggcgatc tgatgttacc
     1921 gataagcgat ttgtgggccg gaaggctgca aaaaatgggc tgcccgaggg aaaaaatcgc
     1981 cgtatcgcgc atgggcgtag atatgacgcg ctttagcccg cgtcccgtga aagcgcccgc
     2041 aacaccgctg gagattattt ccgtcgcacg cttaaccgag aaaaaaggtc tgcatgtggc
     2101 gattgaagcc tgccgtcagt tgaaagagca gggcgtggca tttcgctatc gcattctcgg
     2161 cattggcccg tgggaacgac gcctgcgcac cctcatcgaa caatatcaac tggaagatgt
     2221 ggtagagatg ccgggcttta aaccgagcca tgaagtgaaa gcgatgctcg acgacgcgga
     2281 tgtcttcctg ttgccatcgg taacgggcgc ggatggtgat atggaaggca ttccagtggc
     2341 gctaatggaa gcgatggcgg tcggcattcc ggtggtttct actctgcata gcggaatacc
     2401 ggaactggtg gaggccgaca aatccggctg gctggtgcct gagaacgatg ctcgcgcact
     2461 ggcgcaacga ctggcggcgt ttagccaact taacaccgac gaactggctc cggttgtcaa
     2521 acgtgcgcgc gaaaaagtcg aacacgattt taaccagcag gtgattaacc gagaactcgc
     2581 cagcttgttg caggctttat agaggagaac gtatgccatt taaaaaaatc tcccgacgca
     2641 ccttcctgac ggcaagctcg gcgcttgcct tcctccatac ccctttcgct cgcgcacttc
     2701 ccgcccgaca aagcgttaac attaacgact acaaccctca cgactggatc gcctcattta
     2761 aacaagcctt cagcgaaggg caaacggtcg tcgtgcctgc tggattcgtt tgtgacaata
     2821 tcaacaccgg catcttcatt cctcctggaa aaacgttaca catccttgga agcctgcgcg
     2881 gcaacggcag agggcgattt gtcttacagg acggcagcca ggtgacaggg gaggagggcg
     2941 gcagtatgca taacatcacc ctggatgtgc gtggttctga ctgcaccatc aaagggctgg
     3001 cgatgagcgg ctttggcccg gtaacgcaga tttatatcgg cggcaaaaac aaacgggtca
     3061 tgcgcaacct gaccatcgat aacctcactg tcagccacgc taattacgcc atcttacgcc
     3121 agggatttca taaccagata attggtgcca acatcaccaa ctgtaaattt agtgatttac
     3181 agggggacgc tattgaatgg aacgtggcga ttaacgacag tgatattttg atctccgacc
     3241 atgtcatcga gcgcatcaac tgtaccaacg gcaaaatcaa ctggggcatt ggcataggcc
     3301 ttgcgggaag cacttacgat aataattacc cggaagatca ggcagtgaaa aactttgtcg
     3361 tggcgaatat cacgggatcg gattgtcggc agttgatcca tgttgaaaat ggcaaacatt
     3421 ttgttatccg taatatcaaa gcccgcaata tcacgccgga tttcagtaag aaagcaggca
     3481 ttgataacgc gacagttgct atttacggtt gtgacaattt cgtgattgat aatattgaaa
     3541 tgattaatag cgccgggatg ttaatcggct atggggtaat taaaggcaaa tatctctcga
     3601 taccgcaaaa tttccgagtg aataatattc aactggataa tacccacctt gcttataaat
     3661 tgcgcggtat ccaaatctcc gccgggaatg ccgtctcctt tgtggcgctg actaacattg
     3721 agatgaagcg tgcgtcgctt gagctataca ataaaccgca acaccttttt atgcgtaata
     3781 tcaatgtgat gcaggaatcc tcagttggac ccgcattgag catgaacttc gacatgcgca
     3841 aagacgttcg cggcgtcttt atggcgaaaa aagaaacact gctgtctctt gcaaatgttc
     3901 atgcggtgaa tgagaaagga caaagctccg tcgatatcga cagggttaat caccatattg
     3961 ttaatgtgga aaagattaac tttagattgc cggaacggag agagtagatt tgcgaccatt
     4021 cctggaaaaa tggagccata cttaggaaca atgctactgc aatccacaac gaagcggcgt
     4081 aacatcacaa gtaattcagt aatcaattca gggtaattga tgctggcgaa aaaaatcgaa
     4141 caagctataa ttcagcaacc attttacagg tggatgaaat aatgacgaat ttaaaagcag
     4201 ttattcctgt agcgggtctt gggatgcata tgttgcctgc cactaaggcg attcccaaag
     4261 agatgctacc gatcgtcgac aagccaatga ttcagtacat tgttgacgag attgtggctg
     4321 cagggatcaa agaaatcctc ctggtaactc acgcgtccaa gaacgcggtc gaaaaccact
     4381 tcgacacctc ttatgaatta gaatctctcc ttgagcagcg cgtgaagcgt caactgcttg
     4441 cggaagtgca gtccatctgt ccaccgggcg tgaccattat gaacgtgcgt cagggcgaac
     4501 ctttaggttt gggccactcc attttatgtg cacgacctgc cattggtgac aatccatttg
     4561 tcgtggtgct gccagacgtt gtgatcgacg acgccagcgc cgacccgctg cgctacaacc
     4621 ttgctgccat gattgcgcgc ttcaatgaaa caggacgcag ccaggtgctg gcaaaacgta
     4681 tgccgggtga cctctctgaa tactctgtca ttcagaccaa agaaccgctg gatcgcgaag
     4741 gtaaagtcag tcgcatcgtc gaatttatcg aaaaaccgga tcagccgcag acgctggact
     4801 cagatatcat ggccgtgggc cgttatgtgc tttctgccga tatttggccg gaacttgaac
     4861 acactcagcc tggtgcatgg gggcgtattc agctgactga tgccattgcc gaactggcga
     4921 aaaaacagtc cgttgatgcc atgctgatga ctggtgacag ctacgattgc ggtaaaaaaa
     4981 tgggttatat gcaggcgttc gtgaagtatg ggctgcgcaa cctcaaagaa ggggcgaagt
     5041 tccgcaaagg gattgagaag ctgttaagcg aataatgaaa atctgaccgg atgtaacggt
     5101 tgataagaaa attataacgg cagtgaagat tcgtggcgaa agtaatttgt tgcgaatatt
     5161 cctgccgttg ttttatataa acaatcagga taacaacgag ttagcaatag gattttagtc
     5221 aaagttttcc aggattttcc ttgtttccag agcggattgg taagacaatt agcgtttgaa
     5281 tttttcgggt ttagcgcgag tgggtaacgc tcgtcacatc gtaggcatgc atgcagtgct
     5341 ctggtagctg taaagccagg ggcggtagcg tgcattaata cctctattaa tcaaactgag
     5401 agccgcttat ttcacagcat gctctgaagt aatatggaat aaattaagtg aaaatacttg
     5461 ttactggtgg cgcaggattt attggttctg ctgtagttcg tcacattata aataatacgc
     5521 aggatagtgt tgttaatgtc gataaattaa cgtacgccgg aaacctggaa tcacttgctg
     5581 atgtttctga ttctgaacgc tatgtttttg aacatgcgga tatttgcgat gcagctgtaa
     5641 tggcacggat ttttgctcag catcagccgg atgcagtgat gcacttggct gctgaaagcc
     5701 atgttgaccg ttcaattaca ggccctgcgg catttattga aaccaatatt gttggtactt
     5761 atgtcctttt ggaagccgct cgcaattact ggtctgctct tgatagcgac aagaaaaata
     5821 gcttccgtct tcatcatatt tctactgacg aagtctatgg tgatttgcct catccagatg
     5881 aagtaaataa tacagaagaa ttacccttat ttactgagac gacagcttac gcgccaagca
     5941 gtccttattc cgcatccaaa gcatccagcg atcatttagt ccgcgcgtgg aaacgtacct
     6001 atggtttacc gaccattgtg actaattgct ctaacaatta tggtccttat catttcccgg
     6061 aaaaattgat tccattggtt attctcaatg ctctggaagg taaagcatta cctatttatg
     6121 gtaaagggga tcaaattcgc gactggctgt atgttgaaga tcatgcgcgt gcgttatata
     6181 ccgtcgtaac cgaaggtaaa gcgggtgaaa cttataacat tggtgggcac aacgaaaaga
     6241 aaaacataga tgtagtgctc actatttgtg atttgctgga tgagattgta ccgaaagaga
     6301 aatcttatcg tgagcaaatc acttatattg ccgatcgtcc gggacacgat cgccgttatg
     6361 cgattgatgc tgagaagatt ggtcgcgaat tgggatggaa accacaggaa acgtttgaga
     6421 gcgggattcg gaagacagtg gaatggtacc tgtccaatac aaaatgggtt gataatgtga
     6481 aaagtggtgc ctatcaatcg tggattgaag agaactatga gggccgccag taatgaatat
     6541 cctccttttt ggcaaaacag ggcaggtagg ttgggaacta cagcgtgctc tggcacctct
     6601 gggtaatttg attgctcttg atgttcactc cactgattat tgtggtgatt ttagtaattc
     6661 tgaaggtgta gctgaaactg tcaaaaaaat tcgccctgat gttattgtta atgcggctgc
     6721 tcataccgcg gtagataagg ctgagtcaga acccgaattt gcacaattac tcaatgcgac
     6781 aagtgttgaa gcgattgcaa aagcagccaa tgaggtcggt gcatgggtta ttcactactc
     6841 tactgactac gtatttccgg gaaccggtga aataccatgg caggaggcgg atgcaaccgc
     6901 accgctgaac gtttatggtg aaaccaagtt agccggggaa aaagcattac aagagcattg
     6961 tgcgaagcat cttattttcc ggaccagctg ggtctatgca ggtaaaggaa ataacttcgc
     7021 caaaacgatg ttgcgtctgg caaaagagcg tgaagaatta gctgttatta acgatcagtt
     7081 tggtgcgcca actggcgcag agttactggc tgattgtacg gcacatgcca ttcgtgtggc
     7141 actgaataaa ccggaagtcg caggcttgta ccatctggta gctagtggta ccacaacgtg
     7201 gcacgattat gctgcgctgg tttttgaaga ggcgcgcaaa gcaggcattc cccttgcact
     7261 caacaagctc aacgcagtac caacaacagc ctatcctaca ccagctcgtc gtccgcataa
     7321 ctctcgcctt gatacagaaa aatttcagca gaactttgcg cttgtcttgc ctgactggca
     7381 gattggcgtg aaacgcatgc tcaacgaatt atttacgact acagctattt aatagttttt
     7441 gcatcttgtt cgtgatggtg gagcaagatg aattaaaagg aatgatgaaa tgaaaacgcg
     7501 taaaggtatt attttagcgg gcggttctgg tactcgtctt tatcctgtaa ctatggctgt
     7561 cagtaaacag ctattaccga tttatgataa accgatgatc tattatccgc tctctacact
     7621 gatgttagcg ggtattcgcg atattctgat tatcagtacg ccacaggata ctcctcgttt
     7681 tcaacaactg ctgggtgacg ggagccagtg ggggctaaat cttcagtaca aagtgcaacc
     7741 gactccagat gggcttgcgc aggcgtttat tatcggtgaa gagtttatcg gtggtgatga
     7801 ttgtgctttg gttcttggtg ataatatctt ctacggccac gatctgccga agttaatgga
     7861 cgtagctgtt aacaaagaaa gtggtgcaac agtatttgct tatcacgtaa atgatcctga
     7921 acgctacggt gtcgttgagt ttgataaaaa cggtacggca ataagcctgg aagaaaaacc
     7981 gttacaacca aaaagtaatt atgcggtaac cgggctttat ttctatgaca acgacgttgt
     8041 cgaaatggcg aaaaacctta agccttcttc ccgtggtgaa ctagaaatta ccgatattaa
     8101 ccgtatttat atggaacagg ggcgtttatc tgttgccatg atgggccgtg gttatgcatg
     8161 gctggacacg gggacacatc aaagcctgat tgaggcaagc aacttcattg ccaccattga
     8221 agagcgccag ggactaaagg tttcctgccc agaagaaatt gcttaccgta aaggatttat
     8281 tgatgctgag caggtgaaag tattagctga accgctgaaa aaaaatgctt atggtcagta
     8341 tctgctaaaa atgattaaag gttattaatt aaatgaacgt aattaaaaca gaaatttctg
     8401 atgtgctgat ttttgaacca aaagtttttg gcgatgagcg cggcttcttt tttgaaagct
     8461 ttaaccagaa agtatttgaa gaagctgtag gccgcaaagt tgaatttgtc caagataacc
     8521 attcgaagtc tagtaaaggt gttttacgcg ggctgcatta tcagttagaa ccgtatgcgc
     8581 aagggaaatt ggtacgttgc gctgttggtg aggtttttga cgtagcggtt gatattcgta
     8641 aatcgtcacc aacttttggc aaatgggttg gggtgaattt atctgctgag aataagcgcc
     8701 agttgtggat acctgaagga ttagcacatg ggtttttggt gctgagtgag acggcggagt
     8761 ttttatataa aacgaccaat tattatcatc cagagagtga tagagggatt atttgggatg
     8821 atcctgacat tgacgtaaag tggcctttaa gtatccataa accgatttta tctataaaag
     8881 atgaaaaaca aaagatgttt aaagaaatga tagcgttgga gaaatggaat ggagaatact
     8941 aattattata agttagctat cttaattgta ctttatggaa aagaagtaga acagtgtgaa
     9001 acatttactc atatagatag agttctaaaa aaaaatagga tgtcaaagga tgatattatt
     9061 ctgtgtcttt ggaataatgg cccaagagaa atcgcattta atctgactac agattactat
     9121 accttaaaag tttgtcaaac aataaataat gaatcattaa gttatatata taatttattt
     9181 attaatgagg tggagtcaga aaaatatatt ttactagatc atgattccat attgaaatat
     9241 gattatgttt tggaatgtct taattctaca tctgattgta tcattcctaa tataatagta
     9301 ggaaacaata ttcaaagtcc taaaacatat agcattcaat cgtttaacaa gggaaataca
     9361 gtgcttgcta ttgggagtgg aatttgtata tctaaaaaat tatgtgagaa acttcgacaa
     9421 gtatttggtg atgtttttga ttctaggttt tatttttatg gtgttgattc aacttttttt
     9481 ctacggctga ataaaattgg ggaaagtgag tttataaaaa tcagtgagtg cgttgtattg
     9541 cattcattgt ctagactaga aaatgaagct ataaaagtaa agcaatttag aattaaagaa
     9601 agaagttatg atcaaggatt aacattaaga tattatttct gtaaatttac gttttttact
     9661 gtcgcaaaaa aacttcttgc gaagttgatt tttaataaaa gtgatataag tttaatatat
     9721 tttgcaaaag catattttag cggtaagcat tataagagcg gtaaatcatg aagtttatta
     9781 ttattggcta tagagatgcg tatttttgga atgaatatgg gacatctgtt agagatttac
     9841 aaattgcaga aattctatct agaaagtatc atgttacatt tataaataga cctgtgtcga
     9901 tatatgaaag attaaaaaat aagaagaaag caaaaagtga gtataaagca tatagtaata
     9961 ggataacttt tattgaccat acaagttatg atttaatcgg accgttattt ggccgcatat
    10021 ggacgaaata ttgttacaag aagatattaa atagaatatc atcgttattt acagatgatt
    10081 caatcatcat tgattttacc cctatagccg aattagaatt caagaacaat aaacgaataa
    10141 catattggta tgatttaata gataattttt caattcataa tagattttct gaaaaagaaa
    10201 aaaaagctgt tcgagataaa tatgcttatg tagacgaaaa tgctgatata attacagctg
    10261 tttccgacaa agcgataaaa aattttctaa atcctaataa gtatgtaatg ccaaatggtg
    10321 tttatcttga aaaccaaaat catgaatgtt ttatagatgg aactccaaat
//
LOCUS       DADLFA010000145        10338 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000145 DADLFA010000000
VERSION     DADLFA010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10338
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.146"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            121..987
                     /gene="folD"
                     /locus_tag="JQH65_003115"
     CDS             121..987
                     /gene="folD"
                     /locus_tag="JQH65_003115"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HAY6140447.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            989..1201
                     /gene="ybcJ"
                     /locus_tag="JQH65_003116"
     CDS             989..1201
                     /gene="ybcJ"
                     /locus_tag="JQH65_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HAY6140448.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1309..1830
                     /locus_tag="JQH65_003117"
     CDS             1309..1830
                     /locus_tag="JQH65_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY6140449.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMVLMGMLWWRRR"
     gene            complement(1866..3251)
                     /gene="cysS"
                     /locus_tag="JQH65_003118"
     CDS             complement(1866..3251)
                     /gene="cysS"
                     /locus_tag="JQH65_003118"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAY6140450.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQTRAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3425..3919
                     /gene="ppiB"
                     /locus_tag="JQH65_003119"
     CDS             3425..3919
                     /gene="ppiB"
                     /locus_tag="JQH65_003119"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HAY6140451.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            3922..4644
                     /gene="lpxH"
                     /locus_tag="JQH65_003120"
     CDS             3922..4644
                     /gene="lpxH"
                     /locus_tag="JQH65_003120"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HAY6140452.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4762..5271
                     /gene="purE"
                     /locus_tag="JQH65_003121"
     CDS             4762..5271
                     /gene="purE"
                     /locus_tag="JQH65_003121"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAY6140453.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            5268..6335
                     /gene="purK"
                     /locus_tag="JQH65_003122"
     CDS             5268..6335
                     /gene="purK"
                     /locus_tag="JQH65_003122"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAY6140454.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETVLTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YMGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDANYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6472..7371)
                     /locus_tag="JQH65_003123"
                     /pseudo
     CDS             complement(6472..7371)
                     /locus_tag="JQH65_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
     gene            complement(7368..8183)
                     /locus_tag="JQH65_003124"
     CDS             complement(7368..8183)
                     /locus_tag="JQH65_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HAY6140455.1"
                     /translation="MTIIHPLLASSSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     SHPQPLAAAWMQRAEETGLFGPLAMAASDPLTAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARAGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8194..9453)
                     /locus_tag="JQH65_003125"
     CDS             complement(8194..9453)
                     /locus_tag="JQH65_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HAY6140456.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPAFSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGGCLFEGWVKDEAQALAMLEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRTLAPQIARLDHDKQHIAEV
                     IDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(9463..10287)
                     /gene="fdrA"
                     /locus_tag="JQH65_003126"
                     /pseudo
     CDS             complement(9463..10287)
                     /gene="fdrA"
                     /locus_tag="JQH65_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314544.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
BASE COUNT         2276 a         2941 c         2774 g         2347 t
ORIGIN      
        1 aaccttcgca agaggtcgct tcacgcgata aatctgaaac gaaacctgac agcgcgcccc
       61 gcttctgaca aaataggcgc atccccttcg acctacgtaa cagatggaat cctctctctg
      121 atggcagcaa agattattga cggtaaaacg attgcgcagc aggtgcgctc tgaagttgct
      181 caaaaagttc aggcgcgtat tgcagccgga ctgcgggcac caggactggc cgttgtgctg
      241 gtgggtagta accctgcatc gcaaatttat gtcgcaagca aacgcaaggc ttgtgaagaa
      301 gtcgggttcg tctcccgctc ttatgacctc ccggaaacca ccagcgaagc ggagctgttg
      361 gagcttatcg atgcgctgaa tgccgacaac accatcgatg gcattctggt tcaactgccg
      421 ttaccggcgg gtattgataa cgtcaaagtg ctggaacgta ttcatccgga caaagacgtg
      481 gacggtttcc atccttataa cgtcggtcgt ctgtgccagc gcgcgccgcg tctgcgtccc
      541 tgcaccccgc gcggtatcgt cacgctgctt gagcgttaca acattgatac cttcggcctt
      601 aacgccgtgg tgattggcgc atcgaatatc gtcggtcgtc caatgagcat ggaactgttg
      661 ctcgcaggtt gcaccactac cgtgactcac cgtttcacta aaaatctgcg tcaccacgta
      721 gaaaatgccg atctgttgat cgttgccgtc ggcaagccgg gctttattcc tggtgactgg
      781 atcaaagaag gcgcaattgt gattgatgtc ggcatcaacc gtctggaaaa tggcaaagtt
      841 gtgggcgacg tcgtgtttga agacgcggct aaacgcgcct catacattac gcctgttccg
      901 ggcggcgttg gcccgatgac ggttgccacg ctgattgaaa acacgcttca ggcgtgcgtt
      961 gaatatcatg atccacaggg tgagtaacat ggcgacattt tctttaggta aacatccgca
     1021 cgttgagctg tgcgatttgc tgaaactgga aggctggagc gaaagcggcg cgcaggcgaa
     1081 aatcgcgatt gccgaaggcc aggtgaaagt cgacggtgcg gttgaaacgc gcaaacgctg
     1141 caaaatcgtc gccggtcaga cagtgagttt tgcaggtcac agcgtacagg ttgttgcctg
     1201 atccctttcc cggcctgtat caacaggccg gttctctttt cagacttttg ccctctacgc
     1261 cccgttcatc catgctgata gcatcgacat tttgccgaag cgaccatcat gccaaccgtt
     1321 attacgcacg ccgccgttcc cctatgcatt ggtttagggt tgggttcgaa agtcatccct
     1381 ccgcgtctgt tatttgccgg aatcatcctg gcgatgctgc ccgacgccga cgtattgtcg
     1441 tttaaatttg gcgttgctta cggcaatgtt tttggtcatc gcgggtttac ccattcgctg
     1501 gtgtttgcgt ttgttgtccc gctgttatgt gtgctgattg gacgccgatg gttcagggct
     1561 gggcttattc gctgctggct gtttttaacc gtctcgttgc tatcgcacag cttgctggat
     1621 tcggtaacca ctggcggtaa gggcgttggc tggctgtggc cgtggtcaga tgaacgcttt
     1681 ttcgctccct ggcaggtgat taaagtcgcg ccgtttgcat tgtctcgtta caccacgccg
     1741 tacgggcatc aggtgataat ttctgaattg atgtgggtgt ggttgccggg gatggtgctt
     1801 atgggaatgt tgtggtggcg caggcgataa ccggatgcga aaactcgcat ccggcaacag
     1861 cgcaattact tacgacgcca ggtggtccct tgcgggccat cttccagcac gatccccatc
     1921 tcgttaagac ggtcacgcgc cgcatctgcc gccgcccagt ctttcgcttt acgggcatcc
     1981 agacgctgtt gaattaacgc ttcaatctca gccacttcgc tgtcgtctgc ctgcgcgccg
     2041 ctttgcagga acgcttccgg ttcttgctcc agcaggccca atacagcgga aagtttacgc
     2101 aggtgagatg ccattgcatt cgccgctgcc atatcttctg ctttcagacg gtttacttca
     2161 cgcgccatat caaacagtac ggaataggct tccggggtgt tgaaatcgtc gtccatcgct
     2221 tcaataaagc gcgcttcaaa cgcttcgcca ccggcaggcg caacggtttt atctgtgccg
     2281 cgcagcgcag tgtagagacg ctccagcgcc gcacgcgtct gcttcaggtt ctcttcgctg
     2341 tagttcaact ggctgcgata gtggcccgac atcaggaagt aacgcacggt ttccgcgtcg
     2401 tagtatttca gcacatcgcg cacggtaaag aagttaccca gcgatttgga catcttctcg
     2461 cggtcaacca tcaccattcc ggagtgcatc cagtagttca catactgacc atcatgggca
     2521 caggtggact gcgcgatttc gttctcgtgg tgcgggaaca tcaggtctga accgccgccg
     2581 tggatatcaa agtggttacc cagctgcttg cagttcatgg ccgaacattc aatgtgccag
     2641 ccaggacgac ccgcgcccca cggagacggc cagctcggtt cgccctcttt cgacatcttc
     2701 cacagaacga agtccatcgg gttgcgtttg tcatcaacca cgtcaacgcg cgcgccagcc
     2761 tgcagctggt cgagatcctg acgcgacagc acgccataag tcggatcggt cgggacgtcg
     2821 aacatcacgt cgccgttgtc agccacataa gcgtgacctt tggcgatcag ttgttcagtg
     2881 agttcaataa tttctgcgat atggtgcgtc gcgcgcggct ccatatccgg gcgcagaatg
     2941 ttcaaagcat caaaatcttt gtgcatttcg gcgatcatgc gatccaccag cgccacaaag
     3001 ctttcgccat tttcattggc gcgtttgatg attttgtcgt cgatatcggt aatgttgcgc
     3061 acatacttca gcttatagcc gaggaaacgc agatagcgcg caaccacgtc aaaagcaaca
     3121 aaggtacgcc cgtgaccgat atgacagaga tcgtaaacgg tgattccaca cacgtacatg
     3181 ccgacttccc cggcgtgaat aggcttaaat tcctcttttt ggcgtgtcag agtattgaag
     3241 atttttagca tcgaagattc cgtttagaca tgtgtgggta attgagttgc gtataatacc
     3301 catatttccc gcgcgaatca gcatacattg cgtgatgatc cgatcgtgcg gttatgctat
     3361 aacaccaccc tatatatgac ccgaaccggg ttgaagcacc aatcaaacgg aacaggatgc
     3421 aaaaatggtt actttccaca ccaatcacgg cgatattgtc atcaaaactt ttgacgataa
     3481 agcacctgaa acagttaaaa acttcctgga ctactgccgc gaaggttttt acaacaacac
     3541 cattttccac cgtgttatca acggctttat gattcagggc ggcggttttg aaccgggcat
     3601 gaaacaaaaa gccaccaaag aaccgatcaa aaacgaagcc aacaacggtc tgaaaaatac
     3661 ccgtggtacg ctggcaatgg cccgtactca ggctccgcac tctgcaactg cacagttctt
     3721 catcaacgtg gttgataacg acttcctgaa cttctctggc gaaagcctgc aaggttgggg
     3781 ctactgcgtg tttgctgaag tggttgaagg catggacgtg gtagacaaaa tcaaaggtgt
     3841 tgcaaccggt cgtagcggta tgcaccagga cgtgccaaaa gaagacgtta tcattgaaag
     3901 cgtgaccgtt agcgagtaat cgtggcgaca ctctttattg cagatcttca tctctgcgtg
     3961 gaagaaccgg cgatcaccgc cggttttctg cgttttttag cgggggaagc tcgcaaggcc
     4021 gacgcgctgt atattcttgg cgatctgttt gaagcgtgga ttggcgacga cgatcccaat
     4081 ccactccatc gccagatggc ggcagcgatc aaagcggtgt ccgattccgg cgttccctgt
     4141 tatttcattc atggcaaccg tgattttctg ctcggcaaac gctttgcccg tgaaagtggc
     4201 atgacgttat tgccggaaga aaaggtgctc gaactttatg gccgccgggt gttgattatg
     4261 catggcgaca cgctgtgcac cgatgacgcg ggttatcagg cttttcgcgc caaagtccac
     4321 aaaccctggc tgcaaatgct attcctcgcc ctgccgttgt ttgtgcgcaa acgcattgcc
     4381 gcgcgaatgc gcgcgaacag caaagaagcc aacagcagta aatcgctggc gatcatggac
     4441 gttaaccaaa acgcagtggt cagtgcgatg gaaaaacatc aggtgcaatg gctgatccac
     4501 gggcataccc atcgcccggc ggtgcatgaa cttatcgcca accagcaacc tgcttttcgc
     4561 gtggtactgg gtgcctggca tacggaaggt tcgatggtga aagtcacggc ggatgacgtt
     4621 gagctgattc attttccgtt ttaaaaaaac cacaactttg ctgatttcac agccacgcaa
     4681 ccgttttcct tgctctcttt ccgtgctatt ctctgtgccc tctaaagccg agagttgtgc
     4741 accacaggag ttttaagacg catgtcttcc cgcaataatc cggcgcgtgt cgccatcgtg
     4801 atggggtcca aaagcgactg ggctaccatg cagttcgccg ccgaaatctt cgaaatcctg
     4861 aatgtcccgc accacgttga agtggtttct gctcaccgca cccccgataa actgttcagc
     4921 ttcgccgaaa gcgccgaaga gaacggttat caggtgatta ttgcgggcgc aggtggcgca
     4981 gcgcacctgc caggcatgat tgccgccaaa acgctggtgc cggtgctggg cgtgccagta
     5041 cagagcgccg cactgagcgg tgtcgatagc ctctactcca tcgtacaaat gccgcgcggc
     5101 attccggtgg gtacgctggc gattggtaaa gctggcgcgg caaacgcggc attactggca
     5161 gcacaaattc tcgcgactca cgataaagag ctacaccagc gtctgaatga ctggcgcaaa
     5221 gcccagaccg acgaagtgct ggaaaatccg gacccgcgag gtgcggcatg aaacaggttt
     5281 gcgtcctcgg taacgggcag ttaggccgta tgctgcgtca ggcaggcgaa ccgttaggca
     5341 ttgctgtctg gccggtcggg ctggacgctg aaccggcggc ggtgcctttt caacaaagcg
     5401 tgattaccgc tgagatcgaa cgctggccgg aaaccgtatt aacccgcgag ctggcgcgtc
     5461 atccggcctt tgtgaaccgc gatgtgttcc cgattattgc tgaccgtctg actcagaagc
     5521 agcttttcga taagctccac ctgccgaccg cgccgtggca gttacttgcc gatcgcagcg
     5581 agtggcctgc ggtgtttgat cgtttaggtg aactggcgat tgttaagcgt cgcactggtg
     5641 gctatgacgg tcgcggtcaa tggcgtttac gcgccaatga aaccgaacag ttaccggcag
     5701 agtgttacgg cgaatgtatt gtcgagcagg gcattaactt ctctggtgaa gtgtcgctgg
     5761 ttggcgcgcg cggctttgat ggcagcaccg tgttttatcc gctgacgcat aacctgcatc
     5821 aggacggtat tttgcgcacc agcgtcgctt ttccgcaggc caacgcacag cagcaggcgc
     5881 aagccgaaga gatgctgtcg gcgattatgc aggagctggg ctatatgggc gtgatggcga
     5941 tggagtgttt tgtcaccccg caaggtctgc tgatcaacga actggctccg cgtgtgcata
     6001 acagcggtca ctggacacaa aacggtgcca gcatcagcca gtttgagctg catctgcggg
     6061 cgattaccga tctgccgtta ccgcaaccag tagtgaataa tccgtcggtg atgatcaatc
     6121 tgattggtag cgatgcgaat tatgactggc tgaaattgcc gctggtgcat ctgcactggt
     6181 acgacaaaga agtccgtccg gggcgtaaag tggggcatct gaatttgacc gacagcgaca
     6241 catcgcgtct gactgcgacg ctggaagcct taatcccgct gctgccgcca gagtatgcca
     6301 gcggcgtgat ttgggcgcag agtaagttca gttaagcagg tgctcatttg attgccggat
     6361 gcggcgtaaa cgccttatcc ggcttaccgc tccggtaccc attgtaggcc tgataagacg
     6421 cgtcaagcgt cgaatcaggc atggtgcacc gattgccgga tgctctcatc actacagcga
     6481 aatacaggtc cccgcttcgc ccgccagcgt ctcttcaatt cgcgataacg ccccaatcca
     6541 cgcgggttta ctacggcttc tgacataacc actcaccgcc gttaccttcg gccccatcga
     6601 accatcgaac catcggcttt ggcaaatggc gctaactcat ccggtgtggc atggcgaatg
     6661 gcacgttgct gcggcgttcc ccagttttca tataccgcat cagcatcggt gaggatcacc
     6721 agtccatcgg cattaatctg ctcggcgagc aacgcagtgg cgagatcttt atcaatcacc
     6781 gcttcactcc ctgccccatc ttccgtcaca ggcacaccgc cgccgccact gcaaatcacc
     6841 acatgcccct ctttgagcaa caactcgatg gcttcgctgt cgagaatttt acgcggttgc
     6901 ggagacgcca ccacccggcg caaatattta ccatcacgtt tcatctgcca gccgtaagcc
     6961 gcttccagtg cctcttgttc ttctggctga taaaccggac caataaattt ctctggctgc
     7021 aaaaacgccg gatcatcagg cgaaacctca atgcgcgtca gcaccgtcgt aacaggcggt
     7081 atctgcggct gtgcgctcaa gctctgcgcc agcatatagc ctcatcccct ggctttccgc
     7141 caccagcaca tccagcggat acggttctac ctctttccac gccagattct gtaacgccag
     7201 cagccccacc tgcggcccgt tgccgtgaac aatcgccagc cgataagaac gggccaggcg
     7261 tgccagcgcg ggtacagcac tggcgatatt gcgatattga ttttccgccg tcagcgcctc
     7321 accgcgctgg agtaaggcgt tgcccccaag agctacaacc agtgttttca tggttttcct
     7381 tgtaataatt gttggccaag ccagaacccc agcagcgtat ctgcccctga cgtatgcccc
     7441 agcgccagca gcgaatcaat cgcaacagcg gtacgtttcg ggcaactcag agcatggaca
     7501 aagtgcagga gtggcgaggc gaaatatcct tgcgcggcat aacgtaaata actgacgctc
     7561 accgctgtgg taacgagttg aagattgtcg gaacaggcaa aaaacgggcg accggcgcgc
     7621 gcatctaaag cgccataata ccaagccgcc agcagcattc cgctcaacgt gtcatcatga
     7681 cttggcgtta atccggggcc tttacccagc cagtgccgcc agtcggtctt aacgccattg
     7741 agcgcggcct gaaaacagtg acgaaactgg cgtaattcag cggtcagcgg atcgcttgcc
     7801 gccatcgcca gtggtccgaa aagtccggtt tcctccgcgc gttgcatcca tgcagctgca
     7861 agcggttgag gatgcgaagg cggcgtaata cgcagcaaac aataacgctg tggctgttta
     7921 accgtgaaac gcccgaggcg aatcccttga gccacaacct gtggtcgttc attgccgcat
     7981 aatccaccgc ataacgcatc gaattgcgcg cggcgaagca cccatcctcc ggggccgaaa
     8041 ccactaccct gacgatgcag cgttaacagt tcgccgcttt ccgtcatcag gttaatcgcc
     8101 cgccgccaca ctcccgctaa ccgccaggac tggcgataat tcggtgcgct actgctggca
     8161 agcagaggat ggatgatcgt cattgcgcgt tcctcaacca atccccatgc tttcagccag
     8221 tgcttccagc gcctgttcaa agcacgccag cggtgcccgc acggtgcctg cgccaatctg
     8281 cccgatcccc gcctctttat gggcgatacc ggtattgatg agtggcgtaa tgccggtttc
     8341 taccacgcga cgaatatcca gccccaggca cgcgccctga aaatcccagc ctgggatctg
     8401 caactgcata ttgcgttcaa ggtaaatttc cgccatctct tcagataccg ccctggccgc
     8461 ttccatgcca cccgcaccga caaagcgcgt tacgccaggc gctgcgatca ttgccgcacc
     8521 tccgatacca aaggtttcgg taatcgcgct atcgcccata tccgggttcg cctgctcctg
     8581 cgagaagccg gtgaaaaaca gaccttgcgg agtgtttaca ggcgcagtaa accagcgttc
     8641 acccagcccg cttacccgaa tcccgaacat attgccgttg cgggtcatag ccgtaacgat
     8701 gctgcctgcg cggatcatcg cgccagcatc catcgccgcc ttgcagtagg ccatcgcgag
     8761 gttgaggaag aactgatcgg tcacgctgag gaaatcgatc acttccgcaa tgtgctgttt
     8821 atcatgatcg aggcgagcaa tttgtggggc cagcgtacgc attaacagtg ctgaggaagc
     8881 aatattgcgt tgatggaact catcgcccat cgtaatgccc tgcgccatca tcgccgtgag
     8941 atcgataccg cgctccatgc gccccagcgc cgcgcttaat actggcatta acacatcgcg
     9001 catccagcga tggcgagtca ggacatcttc gccgtaagcg ccaaaacgca tcactttgcc
     9061 gataccttcg ttgaggttgc agtacgcccg gttgccgtcg gtcacgttct cgaccaccag
     9121 catcggcata ctggcagaag taatgccgcc cattggcccg acggcattca catggtgaca
     9181 aggaatgaag ttcacttccc cctgctccag catcgccagt gcctgcgctt catctttcac
     9241 ccagccttcg aacagacatc cgcccacgca cgcccctttc atgggtccgg tcatctcctg
     9301 ccagcgcatt ggcggcccgg cgtgaagcag tgttttgccc tcgtttagtt cgctgataag
     9361 tgaaaaagcc ggttgcacat ccagccagtg tggacgagca cgacgaattt gttcgataac
     9421 cgcagcattg gcttgcgcca ctgatgtaaa catgggaacc ccttattgca aacgttctaa
     9481 taaacgagcc agttttttat tgccgccggc gactggcgac cattggtaat gcacaaccgg
     9541 tttgctggcg ctttgtagct ccagcgcaaa gctgcgtaat ccgatgttaa tcacggcgac
     9601 gttttccagt aatgacggtg tatgttgctg tgcggcaggc gagagcggat aaattaacgc
     9661 tgccgccagc aaggtggctt ccggcagcga actcactacc gcgatccccg catcttccag
     9721 cgtggcgatt tgctgcgagc ggcattgcgg gtcacgttca gtgcctgtca ccgtggcaat
     9781 ggcatacagt ggttgattat ctgaacgcgc ggcacaggct ttttgctagg cgcttaccag
     9841 cgaggcggca ggatcggcag tcgcaccgaa gccaatcacg acatcaagta gcaatacgcg
     9901 cacttgtggt ttagcgccga gatcggcaat taactggttg cgtaaggctg ggtcgatcat
     9961 cggatgggga cgcccgacgg tgtagaaatc atcgccgagg tcaataatct ggtgaccatc
    10021 ggcgtcgagc atcatgccgt gatgatgggt atcgtcggct tccacgccaa ggtgtccagc
    10081 aagtaatccc gccgcttcgg cagccagcgt accgccggta tacaaaccgc aaataaatcc
    10141 gctgctgaca ggcgctattg cgttacgtcg cgccgtgacg cgtgaaagca ggcaagccag
    10201 gcgtgcggcc tcatccagcg aggaggcaaa ccagacattc tcgtcgcggg ccaccgccgg
    10261 ggtataacct aaaaacagcg ccaccatcgg tttgccggtt gctttcatgg cattaacaat
    10321 tttcagacgc acagcttc
//
LOCUS       DADLFA010000146        10282 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000146 DADLFA010000000
VERSION     DADLFA010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10282
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.147"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..1961
                     /gene="acnA"
                     /locus_tag="JQH65_003127"
     CDS             <1..1961
                     /gene="acnA"
                     /locus_tag="JQH65_003127"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HAY6140457.1"
                     /translation="QPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLRKHGVVGK
                     FVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSEDQVELVE
                     KYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKAFAASNEL
                     EVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLLAKKAVTL
                     GLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNSGPLPDPI
                     ETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNINLAAEPI
                     GHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEINVTRSDTY
                     GWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGSIKPDSPA
                     GRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTRHLPDSDV
                     VSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWTAKGPRLLGIRVVIAESFERIHRSN
                     LIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADGSQEVVPC
                     RCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(2025..2615)
                     /gene="ribA"
                     /locus_tag="JQH65_003128"
     CDS             complement(2025..2615)
                     /gene="ribA"
                     /locus_tag="JQH65_003128"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HAY6140458.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            2785..3549
                     /gene="pgpB"
                     /locus_tag="JQH65_003129"
     CDS             2785..3549
                     /gene="pgpB"
                     /locus_tag="JQH65_003129"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HAY6140459.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLLWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            3698..4006
                     /gene="lapA"
                     /locus_tag="JQH65_003130"
     CDS             3698..4006
                     /gene="lapA"
                     /locus_tag="JQH65_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HAY6140460.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            4013..5182
                     /gene="lapB"
                     /locus_tag="JQH65_003131"
     CDS             4013..5182
                     /gene="lapB"
                     /locus_tag="JQH65_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HAY6140461.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNRARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            5375..6112
                     /gene="pyrF"
                     /locus_tag="JQH65_003132"
     CDS             5375..6112
                     /gene="pyrF"
                     /locus_tag="JQH65_003132"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAY6140462.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSDAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            6112..6438
                     /gene="yciH"
                     /locus_tag="JQH65_003133"
     CDS             6112..6438
                     /gene="yciH"
                     /locus_tag="JQH65_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HAY6140463.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(6564..6782)
                     /gene="osmB"
                     /locus_tag="JQH65_003134"
     CDS             complement(6564..6782)
                     /gene="osmB"
                     /locus_tag="JQH65_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HAY6140464.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(7051..7800)
                     /locus_tag="JQH65_003135"
     CDS             complement(7051..7800)
                     /locus_tag="JQH65_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY6140465.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEVIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            complement(7890..8063)
                     /locus_tag="JQH65_003136"
     CDS             complement(7890..8063)
                     /locus_tag="JQH65_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6140466.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            complement(8210..10195)
                     /gene="pdeR"
                     /locus_tag="JQH65_003137"
                     /pseudo
     CDS             complement(8210..10195)
                     /gene="pdeR"
                     /locus_tag="JQH65_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
BASE COUNT         2608 a         2440 c         2622 g         2612 t
ORIGIN      
        1 gccagccggt ttccatgctt atcccggacg tagtgggctt taaacttacc ggtaaattac
       61 gtgaaggtat taccgccaca gacctggttc tcactgttac ccaaatgctg cgcaaacatg
      121 gcgtggtggg gaaattcgtc gaattttatg gtgatggtct ggattcacta ccgctggcgg
      181 atcgcgccac cattgccaat atgtcgccag aatatggtgc cacctgtggc ttcttcccaa
      241 tcgatgctgt aaccctcgat tacatgcgtt taagcgggcg cagcgaagat caggttgagt
      301 tggtcgaaaa atatgccaaa gcgcagggca tgtggcgtaa cccgggcgat gaaccgattt
      361 ttaccagtac gttagaactg gatatgaatg acgttgaagc gagcctggca gggccaaaac
      421 gcccacagga tcgcgttgca ctgcccgatg taccaaaagc atttgccgcc agtaacgaac
      481 tggaagtgaa tgccacgcat aaagatcgcc agccggtcga ttatgttatg aacggacatc
      541 agtatcagtt acctgatggc gctgtggtca ttgctgcgat aacctcgtgc accaacacct
      601 ctaacccaag tgtgctgatg gccgcaggct tgctggcgaa aaaagccgta actctgggcc
      661 tcaagcggca accgtgggtc aaagcgtcgc tggcaccggg ttcgaaagtc gtttctgatt
      721 atctggcaaa agcgaaactg acaccgtatc tcgacgaact ggggtttaac cttgtgggat
      781 acggttgtac cacctgtatt ggtaactctg ggccgctgcc cgatcctatc gaaacggcaa
      841 tcaaaaaagg cgatttaacc gtcggtgcgg tgctgtccgg caaccgtaac tttgaaggcc
      901 gtatccatcc gctggttaaa actaactggc tggcctcgcc gccgctggtg gttgcctatg
      961 cgctggcggg aaatatgaat atcaacctgg ctgctgagcc tattggtcat gatcgtaaag
     1021 gcgacccggt ttatctgaaa gatatctggc catcggcaca ggaaattgcc cgtgcggtag
     1081 aacaagtctc cacagaaatg ttccgtaaag agtacgcaga agtttttgaa ggcacagcag
     1141 agtggaagga aattaacgtc acacgatccg atacctacgg ttggcaggag gattcaacct
     1201 atattcgctt atcgcctttc tttgatgaaa tgcaggcaac accagcacct gtggaagata
     1261 ttcacggtgc gcggatcctc gcaatgctgg gggattcagt caccactgac catatctctc
     1321 cggcgggcag tattaagccc gacagcccgg cgggtcgata tctacaaggt cggggtgttg
     1381 agcgaaaaga ctttaactcc tacggttcgc ggcgtggtaa ccatgaagtg atgatgcgcg
     1441 gcaccttcgc caatattcgc atccgtaatg aaatggtgcc tggcgttgaa ggggggatga
     1501 cgcggcattt acctgacagc gatgttgtct ctatctatga tgccgcgatg cgctataagc
     1561 aggagcaaac gccgctggcg gtgattgccg ggaaagagta tggatcaggc tccagccgtg
     1621 actggacggc aaaaggtccg cgtctgcttg gtattcgtgt ggtgattgcc gaatcgtttg
     1681 aacgaattca ccgttcgaat ttaattggca tgggcatctt gccgctggaa tttccgcaag
     1741 gcgtgacgcg taaaacgtta gggctaaccg gggaagagaa gattgatatt ggcgacctgc
     1801 aaaatctaca acccggcgcg acggttccgg tgacgcttac gcgcgcggat ggtagccagg
     1861 aagtcgtacc ctgccgttgt cgtatcgaca ccgcgacgga gttgacctac taccagaacg
     1921 acggcatttt gcattatgtc attcgtaata tgctgaagta acaagtattt gcttgccggt
     1981 tattttgctt ccggcaagca aattaattcc acaatgcgag agggttattt gttcagcaaa
     2041 tgacccattt tctcggcttt ggtatcgaga taatgttcgt tattggggtt acgacctaca
     2101 atcaatggta cgcgttcaac aatattaatc cctgcttcgg tcagaatttc gacttttttc
     2161 gggttattgg ttaacaagcg gacttcattg acgccaagga gtttgaacat atcagcgcaa
     2221 agagtgaagt cgcgctcatc agcggcgaag cctaactggt ggttagcctc tacggtatcg
     2281 taaccttgat cctgcagtgc gtaagcgcgg attttattca gcagaccaat gttacgacct
     2341 tcctgacggt gatacagcaa aataccacgg ccttcctcgg caatttgcgt caatgccgct
     2401 tcgagctgga agccacaatc gcagcgcaag ctgaacaggg cgtcaccggt cagacattcg
     2461 gaatggacgc gcgcaagtac cggggtatgc ccggaaatat cgccatagac tagcgcgaca
     2521 tgatcgtgtc cggttgccag ttcttcaaat cccaccatca ggaaatcgcc ccatggggtt
     2581 ggcagtttgg cttctgccac acgtttaagc tgcatgaaat tctccagata atgctggttc
     2641 tgtattggct tattttgcca taacgagaag gggttcacct aatcacgacg cgtcgatcgt
     2701 tcacggaatg gcacaaatct gtcaattttt cctggaactg gcgttttcag ttatgattgt
     2761 gggacttatc aaaaaggaga ggccatgcgt tcgattgcca gacgtaccgc agtgggagct
     2821 gcactattgc ttgtcatgcc agtagccgta tggatttctg gctggcgttg gcaacctgga
     2881 gaacaaagtt ggctactaaa agcggctttt tgggttactg aaactgtcac ccagccctgg
     2941 ggcgtcatta cacatttgat tttattcggc tggtttctct ggtgtctgcg ttttcgcatt
     3001 aaggctgcct ttgtattatt tgccattctg gcggccgcaa tccttgtggg acaaggcgtt
     3061 aaatcctgga tcaaagacaa agtccaggaa ccacgacctt ttgttatctg gctggaaaaa
     3121 acacatcata ttccggttga tgagttctac actttaaagc gagcagaacg cggaaatcta
     3181 gtgaaagaac agttggctga agagaaaaat atcccacaat atttgcgttc acactggcag
     3241 aaagagacgg ggtttgcctt tccttccggt cacacgatgt ttgctgccag ttgggcactg
     3301 ctggccgttg gtttgctgtg gccgcgtcgg cgaacgttaa ccattgctat cttgctgctc
     3361 tgggcaacgg gagtcatggg aagccgcctg ctgctcggga tgcattggcc acgcgatctg
     3421 gtagtagcta cattgatttc gtgggcgctg gtggcggtgg caacgtggct tgcgcaacga
     3481 atttgtgggc cattaacacc acctgcggaa gaaaatcgcg aaatagcgca acgagaacaa
     3541 gaaagttaac gctggttgat tttccgaatt tagcccttaa atcatcaaca atgcgcgtgg
     3601 atgccatttc gcagacggcg cgaaaatggt actttaaagg gctattgcgg taagttgacc
     3661 ataatttatt cgctctaacc acataacggg aagtaatgtg aaatatttac tcattttctt
     3721 actggtgtta gcgatcttcg tgatttcggt cacgttgggt gcgcagaacg atcaacaggt
     3781 gacgtttaat tatctgttag cgcaagggga gtaccgtatt tccacattgc tggcggtatt
     3841 gtttgctgcg gggtttgcta tcggttggtt gatttgtggc ctgttctggc tgcgagttcg
     3901 tgtttccctg gcgcgcgctg aacgtaaaat aaagcgactg gaaaaccagc tttcacccgc
     3961 gactgacgtg gctgtagtgc cgcactcgtc agcggcgaag gaataacttt ctatgctgga
     4021 gttgttgttt ctgcttttgc ctgtagccgc tgcctatggc tggtatatgg gccgcagaag
     4081 tgcgcaacaa aacaagcaag atgaagccaa ccgcttgtcg cgtgattacg tagcgggggt
     4141 taacttcctg cttagtaatc aacaggataa agcggtagac ctgtttctcg atatgcttaa
     4201 agaggataca ggcaccgttg aagcccacct tacgctcgga aacctgttcc gttcgcgtgg
     4261 cgaagttgat cgcgctattc gcatccatca gaccctaatg gaaagcgcct cgctgaccta
     4321 tgaacagcgt ctgttggcga ttcaacaact agggcgtgat tacatggccg ccgggttata
     4381 cgaccgcgcg gaagacatgt tcaatcagct gaccgatgaa actgacttcc gcattggcgc
     4441 tctgcaacag ttgctacaaa tctaccaggc taccagtgag tggcagaaag caattgatgt
     4501 tgccgaacgc ctggtgaagc tgggtaaaga taaacagcgc gtcgaaattg cccatttcta
     4561 ctgtgagtta gccctgcagc atatggccag cgacgatctc gatcgtgcca tgaccttgct
     4621 aaaaaaaggg gcggcggcag ataaaaacag agcccgcgta tccatcatga tgggacgcgt
     4681 gtttatggcg aaaggagaat acgccaaagc cgtcgaaagt ttgcaacgcg tgatatccca
     4741 ggacagagaa ctggtcagcg aaacgctgga aatgttgcaa acctgctatc agcagttggg
     4801 taaaactgcc gaatgggcag aattcctgca acgcgcggtg gaagagaaca ccggtgccga
     4861 tgctgaattg atgcttgctg atatcatcga agcgcgcgac ggtagtgagg ccgcacaggt
     4921 ctatattacg cgccagcttc agcgtcatcc gaccatgcgt gtgttccata agttaatgga
     4981 ttaccactta aatgaagcgg aagaagggcg tgccaaagag agcctgatgg tgctgcgtga
     5041 catggttggc gaaaaggtac gtagtaagcc tcgttatcgc tgccagaaat gtggttttac
     5101 cgcatacacc ctctactggc attgtccgtc ttgtcgggcc tggtcaacca ttaaaccgat
     5161 tcgcggtctt gatggcctgt aatttttaaa aaaatccgac tttagttaca acatgctagt
     5221 tattgatgtt ccattgtgct ccggcaacga cggagcagaa aaagcctgcc aggggaaaaa
     5281 tcgcaactgt taatttttta tttccacggg tagaatgctc gccgtttacc tgtttcgcgc
     5341 cacttccggt gcccatcatc aagaaggtct ggtcatgacg ttaactgctt catcttcttc
     5401 ccgcgctgtt acgaattctc ctgtggttgt tgcccttgat tatcataatc gtgatgacgc
     5461 gctgtccttt gtcgacaaga tcgacccacg cgattgtcgt ctgaaggtcg gcaaagagat
     5521 gtttacattg tttgggccac agtttgtgcg cgaacttcaa cagcgtggtt ttgatatctt
     5581 tcttgacctg aaattccacg atattcctaa cactgcagcg cacgctgtcg ctgctgcagc
     5641 tgacttaggc gtgtggatgg tgaatgttca tgcctctggt ggggcgcgta tgatgaccgc
     5701 agcgcgtgag gcactggttc cgtttggcaa agatgcaccg cttttgattg ctgtaacagt
     5761 gttgaccagc atggaagcta gcgacctggc cgatcttggc gtgacactgt cacctgcaga
     5821 ttatgcagaa cgtctggcgg cactgacgca aaaatgtggc cttgatggtg tggtgtgttc
     5881 tgctcaggaa gctgtgcgct ttaaacaggt attcggtcag gagttcaaac tggttacacc
     5941 gggcattcgt ccgcagggga gtgatgctgg tgaccagcgc cgcattatga cgccagaaca
     6001 ggcgttggcg gctggtgttg attatatggt gattggtcgc ccggtaacgc aatcggtaga
     6061 tccagcgcaa acactgaaag cgatcaacgc ctctttacag cggagtgcat gatgagtgat
     6121 tccaacagcc gtctggtcta ctcaacggaa accggacgta ttgatgagcc aaaagcggcc
     6181 ccagtacgcc ctaaaggcga tggtgtggtg cgtattcagc gtcagaccag tggacgtaaa
     6241 ggtaagggcg tttgcctgat taccggtatc gatctcgatg atgccgaact gacaaaactt
     6301 gcagcggaac tgaagaaaaa atgcggctgc ggcggagcag ttaaagatgg agttattgaa
     6361 atccagggcg ataagcgtga tttattaaaa tcactacttg aagcgaaagg gatgaaggta
     6421 aaactcgcag gcggttaaca taaaaagcca cggatatatc cgtggctttc gaatatttta
     6481 ctgtgtgtat tattcagtta tttcgtactg aacaaaatca agcgtattat aattgcgagt
     6541 cgataatcga catttattta cgattattta ccgacctggt gaccaataac accaccgaca
     6601 gctgcaccac ctaatgtacc caacgtactg ccatcggtca gtactgcacc gcctaatgcc
     6661 cctgcacccg cgccgattgc ggtgttgcgg tcccgtttag accagttaga acaggcactc
     6721 agagacattg ccaaagtaat tgccagaaca gccgcggtca tttttttgct cgttacaaac
     6781 ataatactct ctcctgaatt tatgattcac ggaagtaagc tctctataac tataatagct
     6841 aagaataagc ctggtgaaat tattccgtga aatctgctcg cgcgcagggt tatatcacgc
     6901 aggtgataat gacttcctgt tatatcgctg ataataattt tatatcttaa gcgtgttaat
     6961 aacaggtaaa tagtcttaat tatcaaccag gaatcatctt agagcggatg atttgccaaa
     7021 ctgcaaatca tccgtagaga agggaaatgg ttaaatgtca acgacgtgaa cggtgagttt
     7081 actgtgctca agatgcatca gatcgctggc gcgtattttc gaatcggtaa tcacgcgatt
     7141 gaatcgctca acgggaccga tggagtatga atgtacagca ccaaatttcg agctgtcagt
     7201 caggactatc acttcgcact ctttttccag cacggcattg accacatcgg tacgcatcat
     7261 atcgcgaccg gtgaatccag tttcaggttg ccagccatca ataccaataa atgctttgct
     7321 gaaatgcacc tgctggatgc actggcgtgt caacgggcca accatacttt cgcttttttt
     7381 ctggtacacg ccaccgagca aaatgacttc acaaggcgcg tctttcagca aatgcgcgat
     7441 gtagctgctg accgtgatga tagtgacatt tttcttctgc tcccccagag tccgggcgag
     7501 cagggcattg ctgctgccat tttcgataaa gatggtttcg cccggttgaa ccagtgacgc
     7561 ggcaaactcg gcgagttcac gcttcagcgt atagttgctc atcatacggg tttcgacgtc
     7621 atcactatca agcgaaactg caaagccatg tgcacggcgg aggtaactca gtttttcgag
     7681 ggtgttgaga tcctggcgaa tggtaacttc agaaactcca gtggcttttg ccagatcggt
     7741 tacgctaacc tgaccctggt caatgaccat ctgtagaata gtttgttgtc gggaattcat
     7801 cgctgtaatt cttataacgt tataatacta aataaaaaaa tactgacgcg ccgggcatcc
     7861 tcaaatagga aaacctgaag gcacgattgt taaatctccc acggtgcctt cgggatgtct
     7921 ggcgtagatt cagcaacctg gcgcaagagt tctgctttaa gtatctcgag attatggatc
     7981 gcagagtggt aatcgcctgc cacaaggata tctaacacaa tatcaatacg ttcggcaacg
     8041 cgctgggcat caaattcaga catcaaggac atccttatag caaaagtgga aaatgaataa
     8101 tgcaaaaatt gccgctaaaa gagaagtgtt taacagcaac ggctaattat catccaggaa
     8161 tacgatatat aatgacggga tatagcgcta agtatatata ttcatctact tatgcgcgct
     8221 tcagatagcg tttataccag cgttcgaagg cgacggcggg catcggtttg gcaaacaaaa
     8281 atccttgccg ctcattgatc ccgttcttgg ttaaaaaagc atcttccttt gcactctcta
     8341 caccttcggc gatcacctga agattcaatg cctgggccac agcgacgatc gcccggacca
     8401 gtgactgcga gacaggttgt ttgtgaatat ctcgaacaaa aacctggtca agtttgatgg
     8461 catcgatcgg aaagcgcgcc agttgcgaaa gtgaagagta gccggtacca aaatcatcca
     8521 gatgcacttg cgcacctagt tggctaaatt gttgaataac agacagtgcc agttcatcat
     8581 tctcaatcag acaactctct gtcagttcaa catctatagg gcagtattca aaattgagtt
     8641 cctggagaac ctgtttcagg gcggtgaaaa tggtttgatc ggcgagctga cgtgcagaaa
     8701 tatttaccgc cactcgcagg tttatgcctt tatcccgcca ctttgccacc tagcgtacga
     8761 catcgagaat cacccaacgg cctaaaggca caattagccc tgactcttcg gcgtaggaaa
     8821 tgaagtccaa cggtggaatc aacccacgtt caggtgactg ccaacgtact agtgcttcca
     8881 gactgcgcac ttcgccacgc caggtgattt tcggttgata gtgaataacc aactgatcgt
     8941 tttccagtgc tttacgcaag ttggtatcca gccagagata ttcaaatacc cgttgattca
     9001 tttctggggt aaaaacgcaa aattgtcctc gtccgccttc cttcgctgtg tacattgctg
     9061 tgtcggcgtg acgaataata gccgtgctgt ctgaaccatg ttcgggagag agtgcaatac
     9121 ctactgaaca gctggtataa acttcaatta aaccaatgcg aaaggggagc cgtaagcggg
     9181 tcaaaattcg tgatgccatt gcttccagcg cgctttgtga ggtgttggat gccagtacca
     9241 gaaactcatc cccacctgga cgcgccaaca cctggtcatg ttcgagacag cttaaaatag
     9301 ccaatgacac gtcgcgtaat aactggtcac caaacaaatg cccataggcg tcgttgacct
     9361 ttttgaaatt atccaaatca agatacacaa ccccaacttt attgttatct gcatgattaa
     9421 tagcgtgatc gattaaatcc tgcattgcgt tacgattcgg cagtccggtg atactgtcgg
     9481 tatttgccag aatacgcagt cgctcctgag cgcggcgctc ttcggtaatg tcggtgccgg
     9541 aacagattaa aaaaatctcg cttttgccac tgccgctgtg gacaaattta ttgcgaaaca
     9601 gaaacagccg ctggccttta catgttggta tccacagttc gacttcatat gcattgccgc
     9661 ttcgaaaaaa tacacggtta ttgcgcctgg atgccgcagc ttcacgacgg ctcataaaca
     9721 gtttaaacac gctttggcca atgacgtcgt gttctttcaa cccagtgtaa tcttcacata
     9781 accgattgaa gcgttggata ttcccccggc tgtcgagaat cactattgcg gaatgtgctt
     9841 cagaaactac ctgctcagca aaggaaagcc cgtgtgataa atcacgagca actgccggtg
     9901 tgtcatgcca tgcagacgcg ttgccagccc attcccgttt attgattttt cgtcctacca
     9961 gatgcacaga aagatcgctc tcatcgacgg tcagactcat catcaggctg gaggtgatga
    10021 ccgtcatttc cctgatgcga gcagcctgtt cggcagataa ctgcaaagta cgatcaggtt
    10081 ctgtggtttc ggtggtagaa aagcgcaaaa catcgctgct ttccgtcaac cgccagtatg
    10141 gattgtgcga tccgagaaag ttgtacaacg ttgtggactc cctaacggtt ttcatgatgc
    10201 atatcctccg gttaaataaa aggaacaaca tgacggtaat gactccaact tactgatagt
    10261 gttttatgtt cagataatgc cc
//
LOCUS       DADLFA010000147        10225 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000147 DADLFA010000000
VERSION     DADLFA010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10225
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.149"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            141..2009
                     /gene="kup"
                     /locus_tag="JQH65_003138"
     CDS             141..2009
                     /gene="kup"
                     /locus_tag="JQH65_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HAY6140467.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSLTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2176..2595
                     /gene="rbsD"
                     /locus_tag="JQH65_003139"
     CDS             2176..2595
                     /gene="rbsD"
                     /locus_tag="JQH65_003139"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAY6140468.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2603..4072
                     /gene="rbsA"
                     /locus_tag="JQH65_003140"
                     /pseudo
     CDS             2603..4072
                     /gene="rbsA"
                     /locus_tag="JQH65_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            4077..5042
                     /gene="rbsC"
                     /locus_tag="JQH65_003141"
     CDS             4077..5042
                     /gene="rbsC"
                     /locus_tag="JQH65_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HAY6140469.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGTIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            5067..5953
                     /gene="rbsB"
                     /locus_tag="JQH65_003142"
                     /pseudo
     CDS             5067..5953
                     /gene="rbsB"
                     /locus_tag="JQH65_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            6079..7008
                     /gene="rbsK"
                     /locus_tag="JQH65_003143"
     CDS             6079..7008
                     /gene="rbsK"
                     /locus_tag="JQH65_003143"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAY6140470.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            7012..8004
                     /gene="rbsR"
                     /locus_tag="JQH65_003144"
     CDS             7012..8004
                     /gene="rbsR"
                     /locus_tag="JQH65_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HAY6140471.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(7970..9397)
                     /locus_tag="JQH65_003145"
     CDS             complement(7970..9397)
                     /locus_tag="JQH65_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HAY6140472.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMFIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGSVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(9420..10112)
                     /locus_tag="JQH65_003146"
     CDS             complement(9420..10112)
                     /locus_tag="JQH65_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAY6140473.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKKNFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KTCQALLRSPDK"
BASE COUNT         2488 a         2463 c         2705 g         2569 t
ORIGIN      
        1 aaggggggta tttttctttg agccaggtta atgtggccgc atttaggagt acgattttgc
       61 cgttaatcgt gcatactgtg cgcttttttg tgggccaagg gactaagcac acatttcata
      121 tttcaacgaa agactagtct atgagcactg ataataagca atcattgccc gcgattaccc
      181 tcgcggcgat tggagttgtc tacggcgata ttggtaccag cccgttatat acacttcgtg
      241 aatgtttgtc cggccagttt ggttttggcg ttgaacgcga tgccgtgttt ggctttttat
      301 cgctgatctt ctggctgcta atctttgtgg tttccattaa atatctcacc ttcgtgatgc
      361 gggcagataa cgccggtgag ggggggatcc tgacgttgat gtcgcttgcc gggcgtaata
      421 cgtcggcgcg aaccacatca atgctggtga ttatggggct aatcggcggc agctttttct
      481 atggtgaagt cgtcataaca cccgctattt cggtgatgtc agccattgaa ggtctggaaa
      541 tcgtcgcccc gcagctggat acctggatag ttcccctctc aattatcgtt ctcactttat
      601 tatttatgat tcaaaaacat ggcacggcta tggtcggtaa gctgtttgcg ccgatcatgc
      661 tgacctggtt tttgattctg gcaggactgg ggttacgtag cattattgct aacccggaag
      721 tgctgcatgc gctgaatcca atgtgggcgg tgcatttctt ccttgaatac aaaacggttt
      781 cttttattgc attaggggca gtggtgttgt cgattacggg ggtcgaggcg ctgtatgctg
      841 atatggggca ctttggtaag ttccctattc gcctggcgtg gttcaccgtc gtattacctt
      901 ccttgaccct taattacttc ggtcagggag cgctgttgtt aaagaacccg gaagcgatta
      961 agaacccgtt cttcctgttg gcaccggact gggcgctgat cccgctgctg atcatcgccg
     1021 cactggcgac ggtaattgcc tcgcaggcgg ttatctctgg cgtcttctca ttgacgcgtc
     1081 aggcggtacg tctgggatat ttgtcgccga tgcgcattat tcacacctcc gaaatggagt
     1141 cagggcaaat ctatattccg tttgtgaact ggatgctcta tgtcgcggtc gtgattgtga
     1201 ttgtcagctt tgagcactcc agcaacctgg cggcggcgta cgggattgcg gtgaccggaa
     1261 ccatggtgct gacgtctatt ctctcgacta ccgtggcacg tcagaactgg cactggaata
     1321 agtattttgt tgcgctgatc ctgattgctt tcctttgtgt cgatattcca ttgttcaccg
     1381 ctaacctcga taaactgctc tccggcggtt ggttgccatt gagcctcggt acggtgatgt
     1441 ttatcgtgat gaccacctgg aagagcgagc gtttccgctt gctgcggcgg atgcatgaac
     1501 atggtaactc tctggaagcg atgattgctt cgctggagaa atcaccgcct gttcgcgtgc
     1561 ccgggaccgc ggtgtatatg tcgcgtgcaa tcaacgtcat tccctttgcg ctgatgcata
     1621 accttaaaca taacaaggta ttgcatgagc gggtgattct gttaactctg cgtaccgaag
     1681 acgcgccata tgtccataat gtccgtcggg tacagattga acaactgtcg ctcactttct
     1741 ggcgcgtggt ggcaagttat ggttggcgag aaacgccaaa cgtggaagaa gttttccacc
     1801 gctgcggtct ggaaggatta agttgccgga tgatggaaac ctccttcttt atgtcgcatg
     1861 agtcgttgat cctcggcaaa cgcccgtggt atttgcgtct gcgcggcaag ctgtacttgc
     1921 tgctgcaacg taatgcgctg cgtgcaccag atcaatttga aatcccgcca aacagggtta
     1981 tcgaactggg tactcaggtc gaaatctaac gccagacgcc tcctttctcc ataagggggc
     2041 gtttttgttt tcatggttaa tcaccatgta aaacgtttcg aggttgatca catttccgta
     2101 acgtcacgat ggttttccca actcagtcag gattaaactg cgggtcagcg aaacgtttcg
     2161 ctgatggaga aaaaaatgaa aaaaggcacc gttcttaatt ctgatatttc atcggtgatc
     2221 tcccgtctgg gacataccga tacgctggtg gtgtgtgatg ctggtttacc catccccaaa
     2281 agtacaacgc gtatcgatat ggcattaacc cagggtgtac cttcttttat gcaggtgctg
     2341 ggcgtcgtta caaatgaaat gcaggtcgag gctgccatta tcgcggaaga gatcaaacaa
     2401 cataatccgc aactccacga aacgttgctc actcaccttg agcagctgca aaaacaccag
     2461 ggaaatacca ttgaaattcg ttacaccacg catgaacaat tcaaacaaca aaccgcagaa
     2521 agtcaggcgg taattcgcag cggagaatgt tctccgtatg cgaatatcat tctctgtgct
     2581 ggcgtgacgt tctgaggccg tcatggaagc attacttcag cttaaaggca tcgataaagc
     2641 cttcccgggc gtaaaagccc tctcgggcgc tgcgttaaat gtctatccgg gccgcgtgat
     2701 ggcgctggtg ggcgaaaacg gcgcgggtaa atccaccatg atgaaagtgc ttactggtat
     2761 ctatgctcgc gatgccggca cgcttttatg gctggggaaa gaaacgacat ttaccgggcc
     2821 gaaatcttcc caggaagccg ggattgggat tatccatcag gaactgaacc tgatcccgca
     2881 gttgaccatt gccgaaaaca ttttcctcgg tcgtgagttt gttaatcgct ttggcaaaat
     2941 tgactggaaa accatgtatg ccgaagcgga taaattgctg gctaaactta acctgcgctt
     3001 taaaagcgac aagctggtgg gcgatctttc catcggtgac cagcaaatgg ttgaaatcgc
     3061 caaagtgctg agctttgagt cgaaagtcat cattatggat gaaccgaccg atgcgctgac
     3121 cgataccgaa accgaatccc tgttccgcgt catccgcgaa ctaaaatcgc aaggccgcgg
     3181 tattgtctat atctcccacc gcatgaaaga aatcttcgag atttgcgatg ccgttaccgt
     3241 ttttcgtgac gggcaattta ttgctgagcg cgaagtggca tcactgaccg aagattcgct
     3301 gattgagatg atggtgggtc gcaagctgga agatcaatat ccacacctgg acaaagcgcc
     3361 gggagatatc cgcctgaaag tcgataatct ctgcggacct ggcgttaacg atgtctcttt
     3421 tactttacgc aaaggcgaaa ttcttggcgt ctctggtttg atgggcgcag gtcgtaccga
     3481 actgatgaaa gtgctctacg gcgcactacc gcgcaccagc ggttacgtca ccctggatgg
     3541 gcatgaagtc gttacccgtt caccgcagga tggcctggca aacggcattg tgtatatctc
     3601 cgaagaccgt aaacgtgacg gtttagtgtt gggcatgtca gtaaaagaga acatgtcgct
     3661 gacagcgctg cgctacttca gccgcgctgg cggcagtttg aagcatgccg atgaacagta
     3721 ggctgtgagt gatttcattc gtctgtttaa tgtgaaaaca ccgtcgatgg aacaggcaat
     3781 tggtctgctt tccggtggca atcagcaaaa agtgttgatc cttgatgaac ctacccgtgg
     3841 cgtagatgtc ggcgcgaaaa aagagatcta tcaactgatt aaccagttca aagccgatgg
     3901 cttgagcatc attctggtgt catcggagat gccagaagta ttaggcatga gcgatcgcat
     3961 catcgtcatg catgaagggc atctcagcgg ggaatttact cgtgagcagg ccacccagga
     4021 agtgttaatg gctgccgctg tgggcaagct taatcgcgtg aatcaggagt aaaaaaatga
     4081 caacccagac tgtctctggt cgccgttatt tcacgaaagc gtggctgatg gagcagaaat
     4141 cgcttatcgc tctgctggtg ctgatcgcga ttgtctcgac gttaagcccg aactttttca
     4201 ccatcaataa cttattcaat attctccagc aaacctcggt gaacgccatt atggcggtcg
     4261 ggatgacgct ggtgatcctg acttcgggca tcgacttgtc tgtaggttct ctgttggcgc
     4321 taaccggcgc agttgctgca tctatcgtcg gcattgaagt caatgcgctg gtggctgtcg
     4381 ctgctgctct cgcgttaggt gccgcaattg gtgcggtaac cggggtgatt gtagcgaaag
     4441 gtcgcgttca ggcgtttatc gctacgctgg ttatgatgct tttactgcgc ggcgtgacca
     4501 tggtttatac caacggtagc ccagtgaata ccggctttac tgagaacgcc gatctgtttg
     4561 gctggtttgg tattggtcgt ccgctgggcg taccgacgcc agtctggatc atggggattg
     4621 tcttcctcgc ggcctggtac atgctgcatc acacgcgtct ggggcgttac atctacgcgc
     4681 tgggcggcaa cgaagcggca acgcgtcttt ctggtatcaa cgtcaataaa atcaaaatca
     4741 tcgtctattc tctttgtggt ctgctggcat cgctggccgg gaccattgaa gtggcgcgtc
     4801 tctcctccgc acaacccacg gcggggactg gctatgagct ggatgctatt gctgcggtgg
     4861 ttctgggcgg tacgagtctg gcgggcggaa aaggtcgcat tgttgggacg ttgatcggcg
     4921 cattaattct tggcttcctt aataatggat tgaatttgtt aggtgtttcc tcctattacc
     4981 agatgatcgt caaagcggtg gtgattttgc tggcggtgct ggtagacaac aaaaagcagt
     5041 aataacgact acaggacatc ttgaatatga acatgaaaaa actggctacc ctggtttccg
     5101 ctgttgcgct aagcgccacc gtcagtgcga atgcgatggc aaaagacacc atcgcgctgg
     5161 tggtctccac gcttaacaac ccgttctttg tatcgctgaa agatggcgcg cagaaagagg
     5221 cggataagct tggctataac ctggtggtgc tggactccca gaacaacccg gcgaaagagc
     5281 tggcgaacgt gcaggactta accgttcgcg gcaccaaaat tctgctgatt aacccgaccg
     5341 actccgacgc agtaggtaat gctgtgaaga tggctaacca ggcgaacatc ccggttatca
     5401 ctcttgaccg ccaggcaacg aaaggtgaag tggtgagcca cattgcttct gataacgtac
     5461 tgggtggcaa aatcgccggt gattacatcg cgaagaaagc gggtgaaggt gccaaagtta
     5521 tcgagctgca aggcattgct ggtacatccg cagcccgtga acgtggcgaa ggcttccagc
     5581 aggccgttgc tgctcacaag tttaatgttc ttgccagcca gcagattttg atcgcactaa
     5641 aggtttgaac gtaatgcaga acctgttgac cgctcatccg gatgttcagg ctgtattcgc
     5701 gcagaatgat gaaatggcgc tgggcgcgct gcgcgcactg caaactgccg gtaaatcgga
     5761 tgtgatggtc gtcggatttg acggtacacc ggatggcgaa aaagcggtga atgatggcaa
     5821 actagcagcg actatcgctc agctacccga tcagattggc gcgaaaggcg tcgaaaccgc
     5881 agataaagtg ctgaaaggcg agaaagttca ggctaagtat ccggttgatc tgaaactggt
     5941 tgttaagcag tagttttaat caggttgtat gacctgatgg tgacataaat acgtcatcga
     6001 cagatgaacg tgtaatataa agaaaagcag ggcacgcgcc accctaacac ggtggcgcat
     6061 tttatggaca tcccgaatat gcaaaacgca ggcagcctcg ttgttcttgg cagcattaat
     6121 gctgaccaca ttcttaatct tcaatctttt cctactccag gcgaaaccgt aaccggtaac
     6181 cactatcagg ttgcatttgg cggcaaaggc gcgaatcagg ctgtggctgc tgggcgtagc
     6241 ggtgcgaata tcgcgtttat tgcctgtacg ggtgatgaca gcattggtga gagcgttcgc
     6301 cagcagctcg ccactgataa cattgatatt actccggtca gcgtgatcaa aggcgaatca
     6361 acaggtgtgg cgctgatttt tgttaatggc gaaggtgaga atgtcatcgg tattcatgcc
     6421 ggcgctaatg ctgccctttc cccggcgctg gtggaagcgc aacgtgagcg tattgccaac
     6481 gcgtcagcat tattaatgca gctggaatca ccactcgaaa gtgtgatggc agcggcgaaa
     6541 atcgcccatc aaaataagac tatcgttgcg cttaacccgg ctccggctcg cgaacttcct
     6601 gacgaactgc tggcgctggt ggacattatt acgccaaacg aaacggaagc agaaaagctc
     6661 accggtattc gtgttgaaaa tgatgaagat gcagcgaagg cggcgcaggt actgcatgaa
     6721 aaaggtatcc gtactgtact gattacttta ggaagtcgtg gtgtatgggc tagcgtgaat
     6781 ggtgaaggtc agcgcgttcc tggattccgg gtgcaggctg tcgataccat tgctgccgga
     6841 gataccttta acggcgcgtt aatcacggcg ttgctggaag aaaaaccatt gccagaggcg
     6901 attcggtttg cccatgctgc agctgcgatt gccgtaacac gtaaaggcgc acaaccttcc
     6961 gtaccgtggc gtgaagagat cgacgcattt ttagacaggc agaggtgacg cttggctaca
     7021 atgaaagatg ttgcccgcct ggcgggcgtt tctacctcaa cagtttctca cgttatcaat
     7081 aaagatcgct tcgtcagtga agcgattacc gccaaagttg aagcggcgat taaagaactc
     7141 aattacgcgc catcagccct ggcgcgtagc ctcaaactca atcaaacaca taccattggc
     7201 atgttgatca ctgccagtac caatcctttc tattcagaac tggtgcgtgg cgttgaacgc
     7261 agctgcttcg aacgcggtta tagtctcgtc ctttgcaata ccgaaggcga tgaacagcgg
     7321 atgaatcgca atctggaaac gctgatgcaa aaacgcgttg atggcttgct gttactgtgc
     7381 accgaaacgc atcaaccttc gcgtgaaatc atgcaacgtt atccgacagt gcctactgtg
     7441 atgatggact gggctccgtt cgatggcgac agcgatctta ttcaggataa ctcgttgctg
     7501 ggcggagact tagcaacgca atatctgatc gataaaggtc atacccgtat cgcctgtatt
     7561 accggtccgc tggataaaac tccggcgcgc ctgcggttgg aaggttatcg ggcggcgatg
     7621 aaacgtgcgg gtctcaacat tcctgatggc tatgaagtca ctggtgattt tgaatttaac
     7681 ggcgggtttg acgccatgcg ccaactgtta tcacatccgc tgcgtcctca ggccgtcttt
     7741 accggaaatg acgctatggc tgttggcgtt taccaggcgt tatatcaggc agagttacag
     7801 gttccgcagg atatcgcggt gattggctat gacgatatcg aactggcaag ctttatgacg
     7861 ccaccattaa ccactatcca ccaaccgaaa gatgaactgg gggagctggc gattgatgta
     7921 ctcatccatc ggataaccca gccgaccctt cagcaacaac gattacaact tactccgatt
     7981 ctgatggaac gcggttcggc ttagatttac gctgtctttt gatcaaatta ttaccatcgg
     8041 ttgttttcag aagcatgaac attgctgctg aagcaacagt aataatgccc atcgtgataa
     8101 acgtatagtg gaattgttcg acagtcgttg tgccttccat cccttcataa acgcgaagga
     8161 cggccgcact tacagcaacg cctaaactaa tcgacagttg ctgcgtgacc gccagaacac
     8221 tgttaccgct gctggcgttg tcatcggtca gatcggcaag tgtgatggta ttcatcgcgg
     8281 taaattgcgt cgacatagcc atccctaata taaacaacgg caagatcagc atccatatcg
     8341 ccattgccgg tgattgcaaa gagaactgag cgatcattag cccaataatc accgtgatcc
     8401 ccactaacgt atggcgatag cccagacgac gtaagacttg ggtaaccatc gattttgcaa
     8461 taatggaacc taacgctgtc ggtgccatca tacagccggc aataaacgcc tgataaccaa
     8521 atcctacctg taacatcaat ggcataagga acggtacact accggtcccc agacgggttg
     8581 caatattgcc tacgataccg atcgagaaag tgcgggtttt aaataaatct aatgaaatta
     8641 atgggtttgg cgtgtgtcgc gcatggagaa tatagagaag cagtaacccg atgctggtga
     8701 caattaccgt caaggcaatc cagctggcga caatcttttc cccgaatagc tctattccgc
     8761 ttgagaagag aacaaggctg aggccaaaca gcaaaaagcc agtgatatcg aatctgcgtc
     8821 gtgcggtggt gaaattgggc atatgtttgc gcgcgtaaag aaggcccgca atacctatgg
     8881 ggatattgat taaaaatatc cagtgccagg ttgcccaggt gaccagcacg ccgccaagaa
     8941 cggggcctaa aattggcccc accagacccg gcatggcgac aaaattcaat acaggaagaa
     9001 gttcattacg aggataagca cgcagtaagg ccagccgagc aacaggcatc atcattgcgc
     9061 cgcctatccc ctgaataacc cggaagacaa ccagctgtgg tagcgaatta gaaagtgcgc
     9121 aggccagaga acccaacgtg aacagactca cggcaagggt aaaaatgcga cgcgtaccga
     9181 agcgatcggc tagccatccg cttaccggaa taaacatcgc caccgtcagc gtataactga
     9241 tgatggctga ttgcatcgcg agaggagaac gattaaggct atgagcgatt gcgggtaagg
     9301 cggtattcag aatagtggca tcaagtgcct gcatgaagaa ggccatcgcc gcgatccacg
     9361 gcaaacccgc catactacgc ttctttttat cgctcattca atgtcctgtt atcgggttat
     9421 cacttatcag gtgagcgtag cagcgcctga caagttttaa atgccgcgtc gccatcgctt
     9481 tggataatcg catcgacaat cgcctggtgc agatccagct ttatcactgt gtcgctggta
     9541 attgacgtga agtaagtgtg ataaaccgaa tggaatagcg aggcgaatga ggtcaaaaac
     9601 ggattggcgc tcatttcata gatatgctca tgccaggcca tatcgacttc gatccagcgt
     9661 tcacggcgaa agttcttttt taatgccgcc atttcggcca ttaacgtatt gagatgcgcc
     9721 ttctgttccg cggtgccaac cgttgctgcc agtaggcagg cttgcggctc cagacagata
     9781 cgcataacca gaaagtgatc gatgacctga tgaaagttct cttctgtcat ccaccaggta
     9841 agcaattcct gatcaagaaa attccagttt gattgtggca tgacccgggt accaattcgc
     9901 ggtcgcggta aaaccatccc ttttgccgtt aacgttttga ccgcttcgcg taccgctgta
     9961 cgactcactc caaattgctc gcccagctca atctcaccag gcaaaatggt gccgggttca
    10021 tattcacctt ttaagatgcg ttgcgccagc ttctcagcca gaacatacga aaggtttttc
    10081 tgtgcagcta actgttgtgc gcttaaaggc attactaatc ttcctttttc tttttattcc
    10141 tccttagtat gccaccagga agtgtgatta cggttgcaaa aacggcaaat tgcttgtttt
    10201 atggcacatt aacggggctt ttgct
//
LOCUS       DADLFA010000148        10205 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000148 DADLFA010000000
VERSION     DADLFA010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10205
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.150"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(265..1296)
                     /gene="rsmC"
                     /locus_tag="JQH65_003147"
     CDS             complement(265..1296)
                     /gene="rsmC"
                     /locus_tag="JQH65_003147"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HAY6140474.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            1399..1812
                     /gene="holD"
                     /locus_tag="JQH65_003148"
     CDS             1399..1812
                     /gene="holD"
                     /locus_tag="JQH65_003148"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HAY6140475.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            1781..2227
                     /gene="rimI"
                     /locus_tag="JQH65_003149"
     CDS             1781..2227
                     /gene="rimI"
                     /locus_tag="JQH65_003149"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAY6140476.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKNFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIVVDPDYQRQGLGRVLLKHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            2242..2919
                     /gene="yjjG"
                     /locus_tag="JQH65_003150"
     CDS             2242..2919
                     /gene="yjjG"
                     /locus_tag="JQH65_003150"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HAY6140477.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGIAKPNKKIFDYAL
                     ELAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHLEQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            3010..4599
                     /gene="prfC"
                     /locus_tag="JQH65_003151"
     CDS             3010..4599
                     /gene="prfC"
                     /locus_tag="JQH65_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAY6140478.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            4992..5597
                     /gene="osmY"
                     /locus_tag="JQH65_003152"
                     /pseudo
     CDS             4992..5597
                     /gene="osmY"
                     /locus_tag="JQH65_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
     gene            5724..5885
                     /locus_tag="JQH65_003153"
     CDS             5724..5885
                     /locus_tag="JQH65_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HAY6140479.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            6007..7080
                     /locus_tag="JQH65_003154"
     CDS             6007..7080
                     /locus_tag="JQH65_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HAY6140480.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGCYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVIDAPQANDPTFNNEDLA"
     gene            7077..7859
                     /locus_tag="JQH65_003155"
     CDS             7077..7859
                     /locus_tag="JQH65_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY6140481.1"
                     /translation="MICRFIDTHCHFDFLPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEVGLDL
                     FGDDPQLERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLSRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
     gene            complement(8073..8936)
                     /gene="yjjW"
                     /locus_tag="JQH65_003156"
     CDS             complement(8073..8936)
                     /gene="yjjW"
                     /locus_tag="JQH65_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HAY6140482.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVLWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQHLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(8908..>10205)
                     /locus_tag="JQH65_003157"
     CDS             complement(8908..>10205)
                     /locus_tag="JQH65_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HAY6140483.1"
                     /translation="WLELEGAKDLDDALSLLTILYHHVPSVTSMPVYLGQLDALLQPY
                     VRILTQDEIDVRIKRFWRYLDGTLPDAFMHANIGPSDSPITRAILRADAELKQVSPNL
                     TFIYDPEIPPDDLLLEVAKNICECSKPHIANGPVHDKIFTKGGYGIVSCYNSLPLAGG
                     GSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQIAIIDARCEFLYQQSHFFENSFLV
                     KEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAARYGKEAAANEVGYRISAQLAEFVA
                     NTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPYGDEPDPIIHLQTVAPHHAYYYSGI
                     SDILTLDETIKRNPQALVQLCLGAFKAGMREFTANVSGNDLVRVTGYMVRLSDLEKYR
                     AEGSRTNTTWLGEEATRNTRILERQPRVISHEQQMRFSQ"
BASE COUNT         2409 a         2665 c         2749 g         2382 t
ORIGIN      
        1 ccatcaattc ttaaaaagaa ttgctaccac ggaggcgcat tctagtggtt ttcagctttt
       61 cgtcaatagt taattatcgc cggaggtgta attgctggaa aaatgtccat caggaaacta
      121 gcgtgcaggt ttggtaggca tgcgggggca gatgccagat gcgacgctgg cgcgtcttat
      181 ctggcctacg aagggctaac gtgcaggttt tgtaggtcgg ataaggcgtt tacgccgcat
      241 ccggcaaggc ttgcggcggg atacttaacc ttttttcgcc tggcgggtca taatggcgcg
      301 atataccttg aaacgcccgg tttgcgcaat cacttcatgg aagccaaatg tttcatccag
      361 cacgtccgga taaggcagga aggcgttcgc tacaattcgc agctcgccgc cactattaag
      421 atgacgcacc gcgccgcgaa tcagcgtttg cgccgcatcc aggctggttt gcatcccatc
      481 gtggaacggc gggttggaga tgatcatatc aaaacgacct ttcacctcgg aaaagacgtt
      541 gctggcaaag acttcacctt caacacagtt ggccgcaagt gttgcgcggc tggcttctac
      601 cgctggcgca gagacatcgc acaaggtgag acgaattttc ggcgaatggc gcgcaaaggc
      661 aactgaaagc acccccgcgc cacagccgac atccagcact ttacctttcg tgtgcggagt
      721 taacgtcgag agcagcaact ggctaccgac atccagaccg tcgcggctaa acacgccagg
      781 cagcgttttg accgtcaggc catcgacgct gtattcgccc cagaatttat cggcatcaaa
      841 taccggctgt ttttccagac gaccaaaata gaggccacag cgacgagcgc tgtcgacttt
      901 attcaacggc gcataatctg ccagcatctg ctcggcgctg cgcacgccgc tgcggttctc
      961 gccaacgaca aaaatatctg tccccactgg cagcagagaa agtaaattca tcaactggaa
     1021 ctgggcttcc ggtttgttct tcggccagta gtaaatcagc gtatcgcaat ctgcgacgtc
     1081 atccaccgtg gcgaccagac tgaaacgggc gttatccccc atctggcggc ttaatacctg
     1141 ccagtggtgg aattgctggg tatgagcacg gctggccgcg gtatctaaac gcgcgggcag
     1201 gtcatcctgt aagtctccgg caaacagaat acggctttgt tcgaaatcat cactgtgacg
     1261 cagcaagact tcacttgccg gggtaaatgc agacatggaa tgctcctcaa ttgatactgg
     1321 cggcgattat agccatatgt tggcgcggta tcgacgaatt tgctatattt gcgcccctga
     1381 caacaggagc gattcgctat gacatcccga cgagactggc agttacagca actgggcatt
     1441 acccagtggt cgctgcgtcg ccctggcgcg ttgcaggggg agattgccat tgcgatcccg
     1501 gcacacgtcc gtctggtgat ggtggcaaac gatcttcccg ccctgaccga tcctttagtg
     1561 agcgatgttc tgcgcgcatt aaccgtcagc cccgatcaag tgctgcaact gacgccagaa
     1621 aaaatcgcga tgctgccgca aggcagtcgc tgcaacagct ggcggttggg tactgacgaa
     1681 ccgctatcac tggaaggcgc tcaggtggca tcaccggcgc tcaccgaatt acgggcaaac
     1741 ccaacggcac gcgccgcgtt atggcaacag atttgcacat atgaacacga tttcttccct
     1801 cgaaacgact gatttaccag cggcttacca cattgaacaa cgcgcccacg cctttccgtg
     1861 gagtgaaaaa aactttgcca gcaaccaggg cgagcgttat ctcaactttc agttaacgca
     1921 aaacggcaaa atggcggcgt ttgctattac gcaagtggtg ctggatgaag ctacattgtt
     1981 caatattgtg gtcgatcctg actatcagcg tcagggattg ggaagggtgc tgctgaaaca
     2041 tctgatcgac gaactggaaa aacgcggcgt ggcgacacta tggctggaag tccgtgcttc
     2101 aaacgctgcc gccattgccc tgtacgaaag tttaggcttt aacgaggcga cgattcgccg
     2161 caattactac cccaccacgg acggtcgcga agacgccatc atcatggcgt tgccaatcag
     2221 tatgtaagac aaggtggaat aatgaagtgg gactggattt tctttgatgc cgatgaaacg
     2281 ctgtttacct ttgactcatt caccggcctg cagcggatgt ttcttgatta cagcgtcacc
     2341 tttaccgctg aagattttca ggactatcag gccgttaaca agccactgtg ggtggattat
     2401 caaaacggcg cgatcacttc attacagctt cagcacgggc gtttcgagag ctgggccgaa
     2461 cggctgaaag ttgaagcagg cttgcttaac gatgccttta ttaatgcgat ggcggaaatc
     2521 tgcacgccgc tgccgggcgc ggtttctctg cttaacgcca ttcgtggcaa cgctaaaatc
     2581 ggcatcatca ccaacggttt tagcgccttg cagcaagtgc gtctggaacg gacgggcctg
     2641 cgtgattact ttgatttgct ggtgatttcc gaagaagttg gcattgccaa accgaataag
     2701 aaaattttcg attatgcgct ggaactggcg ggcaatcctg accgttcacg cgtgctgatg
     2761 gttggcgaca ctgccgagtc cgatattctc ggtggcatca acgccgggct tgcgacttgc
     2821 tggctgaatg cgcaccatct cgagcaacca gaaggcatcg cgcccacctg gaccgtttca
     2881 tcgttgcacg aactggagca gctcctgtgt aaacactgat tgcctccccc ccgttgatgg
     2941 gtaaaatagc cgcaattttt cgttttcaac aagcgcggcg cgatgccgct tactcaagaa
     3001 gaaagaatta tgacgttgtc tccttatttg caagaggtgg cgaagcgccg cacttttgcc
     3061 attatttctc acccggacgc cggtaaaact accatcaccg agaaggtgct gctgttcgga
     3121 caggccattc agaccgctgg tacagtaaaa ggccgtggtt ccaaccagca cgctaagtcg
     3181 gactggatgg agatggaaaa gcagcgtggg atctccatta ctacgtctgt gatgcagttt
     3241 ccgtatcacg attgcctggt taacctgcta gacaccccgg ggcacgaaga cttctcggaa
     3301 gatacctatc gtaccctgac agcggtggac tgctgcctga tggttatcga cgctgcaaaa
     3361 ggtgttgaag atcgtacccg taagctgatg gaagttaccc gtctgcgcga cacgccgatc
     3421 ctcaccttta tgaacaaact tgaccgtgat atccgcgacc cgatggaact gctcgatgaa
     3481 gttgagaacg agctgaaaat tggctgcgca ccgatcacct ggccgattgg ctgcggcaag
     3541 ctgtttaaag gcgtttacca cctttataaa gacgaaacct atctttatca gagcggtaaa
     3601 ggccacacga ttcaggaagt ccgtattgtt aaagggctga ataacccgga tctcgacgct
     3661 gcggttggtg aagatctggc acagcagcta cgtgacgaac tggaactggt gaaaggcgcg
     3721 tctaacgagt tcgacaaaga actgttcctt gcgggcgaaa tcactccggt attcttcggt
     3781 actgcgctgg gtaacttcgg cgttgatcat atgctggatg gcctggtgga gtgggcccct
     3841 gcgccgatgc cgcgtcagac tgatacccgt accgtagagg cgagcgaaga caaatttacc
     3901 ggcttcgtat ttaaaattca ggccaatatg gacccgaaac atcgcgaccg cgtggcgttt
     3961 atgcgcgtag tatccggtaa atatgaaaaa ggcatgaagc tgcgccaggt gcgtactgcg
     4021 aaagatgtgg ttatctccga cgcgctgacc tttatggcgg gcgaccgttc gcacgttgaa
     4081 gaagcgtatc cgggcgatat cctcggcctg cacaaccacg gcactattca gatcggcgac
     4141 acctttaccc agggtgagat gatgaagttc accggtattc cgaacttcgc accagaactg
     4201 ttccgtcgta tccgcctgaa agatccgctg aagcaaaaac agctgctcaa agggctggta
     4261 cagctttccg aagagggcgc ggtgcaggtg ttccgtccga tctccaacaa cgatttgatc
     4321 gttggtgctg ttggtgtgct gcagtttgat gtggtggtat cgcgcctgaa gagcgaatac
     4381 aacgttgaag cagtatatga atcagtcaac gttgccactg cccgctgggt agaatgtgcg
     4441 gacgcgaaga aattcgaaga gttcaagcgt aagaacgaaa gccaactggc gcttgatggc
     4501 ggcgataacc tcgcttacat cgctaccagc atggtcaacc tgcgcctggc gcaggaacgt
     4561 tatcccgacg ttcagttcca ccagacccgc gagcattaat tcttgcctcc agggcgcggt
     4621 agccgctgcg ccctgtcaat ttcccttcct tattagccgc ttacggaatg ttcttaaaac
     4681 attcactttt gcttatgttt tcgctgatat cacgagcggt ttcaaaattg tgatctatat
     4741 ttaacaaagt aatgacattt ctgacggcgt taaataccgt tcaatgcgta gatatcagta
     4801 tctaaagccg tcgattgtca ttctaccgat attaataact gattcagagg ctgtaatggt
     4861 cgttattcat cacccatcgc ttttgtgatg gcggccattg acttctgtag agggtgaagt
     4921 ctctccctat tcagcaatgc aacctcgtgt tgccaggctc aaattacgag caaacataca
     4981 ggaataaatc gatgactatg acaagactga agatttcgaa aactctgctg gctgtaatgt
     5041 tgacctctgc cgtcgcgacc ggctctgcct acgcggaaaa caacgcacag actaccaatg
     5101 aaagcgcagg gcaaaaagtc gatagctcta tgaataaagt cggtaatttc atggatgaca
     5161 gcgccatcac cgcgaaagtg aaggcggcgc tggtggatca tgacaacatc aagagcaccg
     5221 atatctctgt caaaaccgag caaaaagtcg tgaccctgag cggtttcgtt gaaagctagg
     5281 ctcaggccga agaggcagtg aaagtggcga aaggcgttga aggggtgacc tctgtcagcg
     5341 acaaactgca cgttcgcgac gctaaagaag gctcggtgaa gggctacgcg ggtgacaccg
     5401 ccaccaccag tgaaatcaaa gccaaactgc tggcggacga tatcgtccct tcccgtcatg
     5461 tgaaagttga aaccaccgac ggcgtggttc agctctccgg taccgtagat tctcaggcac
     5521 aaagtgaccg tgctgaaagt atcgccaaag cggtagatgg tgtgaaaagc gttaaaaatg
     5581 atctgaaaac taagtaattc gtcgtaattc gtcctcccga actttgtcgg gaggcgcgat
     5641 gtgcaccaca ctaaaaatat cgcattgagt gattttcacg ctcatatcaa gcggtgacat
     5701 taactatggt aaaggagacg cttatgtttc gttggggcat catatttctg gttatcgcgt
     5761 taatcgccgc cgcacttggg tttggtggtt tggccggtac cgctgcaggc gcagctaaaa
     5821 ttgtctttgt cgtcgggatt attctgttcc tggtgagttt gttcatgggc cgaaaacgac
     5881 cctagatttc agaccattaa gaaaatttta tccaaagcca gtccagcgga ctggcttttg
     5941 cggttttagc gaataataaa ttgcgttact ttgtcattct ttcacaacaa aaacaggaag
     6001 gcagaggtgg ggcagcgaat acctgtaacg cttggtaata ttgcgccgtt gtcgctaagg
     6061 ccgttccagc ctggacgaat agctctggtg tgcgaaggcg gcggacagcg tggaattttc
     6121 acggctggcg tgctggatga gtttatgcgc gcgcagttta atcctttcga tctttatctc
     6181 ggcacatctg ccggggcgca gaacctctcg gcgtttatct gcaatcagcc cggttacgcg
     6241 cgtaaagtca tcatgcgcta taccacaaaa cgcgaatttt tcgatccatt gcgctttgtc
     6301 cgtggaggaa atcttatcga tctcgactgg ctggtggagg ccaccgcaag ccagatgccg
     6361 ttgcaaatgg ataccgccgc gcggttgttt gacagcggca aatcgtttta tatgtgcgcc
     6421 tgtcgtcagg atgactacgc gcctaattac tttttaccaa ccaaacaaaa ctggctggat
     6481 gtgattcgcg cctccagtgc gatacctggc ttttatcgta gcggagtgtc gctggaaggc
     6541 attaactacc tggatggcgg gatcagtgat gcgattccgg ttaaagaggc ggcaaggcag
     6601 ggcgctaaaa cgttggtcgt cattcgcact gtgccgtcac aaatgtacta cacgccgcag
     6661 tggttcaaac gcatggaacg ctggctgggt gacagtagcc tgcagccgct ggtcaatctg
     6721 gtgcagcatc atgaaaccag ctatcgtgac attcagcaat ttattgagaa accaccgggc
     6781 aagctgcgga tattcgaaat ttatccgccg aagccattac atagtatcgc gcttggcagt
     6841 cggattccgg cgctgcgtga agactataaa cttgggcgtt tatgcggttg ttatttcctc
     6901 gccacggttg gcaagctatt aactgaaaaa gcgccgctta cccgccatct ggtgccagtg
     6961 gtgacgccgg aatcgatcgt tattccgcct gcgccagtcg ccaacgatac gctggttgcc
     7021 gaagtgattg acgctccgca ggcgaacgac ccgacattta acaatgagga tctggcttga
     7081 tttgccgttt tatcgacacc cactgccatt ttgatttcct gccgtttagt ggcgatgaag
     7141 aggccagcct gcaacgcgcg gcacaagcgg gcgtaggcaa gatcattgtt ccggcgacag
     7201 aggcggaaaa ttttgcccgt gtgcaggcgt tagcggaaaa atatcaaccg ctgtatgccg
     7261 cattgggctt gcatccaggt atgttggaaa aacatagcga tgtgtctctt gagcagctac
     7321 agcaggcgct ggaaaggcgt ccggcgaagg tggtggcagt gggggaggtc ggtctggatc
     7381 tcttcggcga cgatccgcaa cttgagaggc agcagtggtt actcgacgaa cagctgaaac
     7441 tggcgaaacg ctacgatctg ccggtgatcc tgcattcacg gcgcacacac gacaaactgg
     7501 cgatgcatct taaacgccac gatttatcgc gcactggcgt ggttcacggt ttttccggca
     7561 gcctgcaaca ggccgagcgg tttgtacagc tgggctacaa aatcggcgta ggcggtacta
     7621 tcacctatcc acgcgccagt aaaacccgcg atgtcatcgc aaaattaccg ctggcatcgt
     7681 tgttgctgga aaccgacgcg ccggatatgc cgctcaacgg ctttcagggg cagcctaacc
     7741 gaccggaaca ggctgtgcgg gtgtttgacg tgctttgcga gttgcgcccg gaaccagaag
     7801 atgagattgc cgaagtgttg cttaataata cgtatgcgtt gtttagcgtt agtgggtagt
     7861 ttacccttcg taggcagtat ccggcagtcg gcgcataatg cctgatgcga cgcttgtcgc
     7921 gtcttatcat gcctacaggt ttgtgccgaa ccgtaggccg gataaggcgt tcacgccgca
     7981 tccggcagtt ggcgcacaat gcctgatgcg acgcttgtcg cgtcttatca tgcctacaag
     8041 tctgtgccga accgtaggcc gtatccggca tgtcacaaat agagcgccgg aaatatcaac
     8101 cggctcaccc cgcgcacctt taacgcatca gccaacggct caacgtcttc cggcgtggcg
     8161 ctcgcccagc tttgcgcctc gccatacacg ccgtgggcat gaaacgcatt caggcgaacc
     8221 ggaacatcgc caagtctctt gataaacgtc gccagctcat cgatgtgatg caaataatcc
     8281 acctggtctg gaattaccag caaacgcagt tccgccagct tgccgcgctc tgcaagcaaa
     8341 cagatgctgc gcttaatctg ctgattatcg cgtccggtta agtgttgatg acattcgctc
     8401 ccccacgctt tgagatcgag cattgcgccg tcacacaccg ggagtaattt ttcccagccg
     8461 gtttcgctca acatgccgtt actgtccacc aggcaggtga ggtggcgcag ttgcggatcg
     8521 tttttgatag cagtaaacag cgccactaca aacggcagtt gggtcgtggc ttcgccgccg
     8581 cttaccgtta tcccttcaat aaacagcacc gctttgcgga catggctaag cacttcgtcc
     8641 acgctcatgg attgcgccat tggcgtggca tgttgcggac acatcttcag gcaggtatca
     8701 cactgctcgc agaccacagc gttccacagc actttgccgt caacaatctg caacgcctga
     8761 tgcggacact gtggcacgca ctctccgcaa tcattgcagc gtcccatcgt ccacggattg
     8821 tgacagtttt tgcagcgcag attgcagccc tgcagaaaca gagccagacg actgcctggc
     8881 ccgtcaacgc aggagaaggg gataatctta ctgactaaag cgcatctgct gttcatggct
     8941 tatcacgcgc ggctggcgtt ccagaatacg agtgttgcgt gtggcttctt cgcccagcca
     9001 ggtggtgttg gtgcgtgaac cttcggcgcg atatttttct aaatccgaca aacgcaccat
     9061 ataaccggta acgcgaacca gatcgttacc gctgacattg gcggtaaatt cacgcattcc
     9121 ggctttaaag gcaccgaggc aaagctgtac cagcgcctgc ggattacgtt tgatggtttc
     9181 gtcgagcgtc agaatgtcgc tgatgccgga ataataataa gcatgatgcg gggcgacagt
     9241 ttgcagatgg atgatcggat ctggctcatc gccatacggc aaacgcgcgc ccggcgtggt
     9301 gccgatatcg gaactgatcc ccgactgtgc gtgtaacatg gcgcgttttt gccagccata
     9361 tttcacgggg gtattggcga caaactccgc cagttgcgcg ctgatgcgat aacccacttc
     9421 atttgcggcg gcttctttac cgtagcgcgc ggcaatccct tctttctcac acagcaagtt
     9481 aaccgcttcc gccagcccat acatgccaaa cattggcaca aaacgttcag ggttaatcag
     9541 cccttctttc accaggaagc tattctcaaa gaagtgtgat tgttgataga ggaattcaca
     9601 ccgcgcatcg atgatggcga tctgctgctg gcagtagtgc ggtagagtgc gcgtaaagaa
     9661 gtcatccagc gattcgctgc gctcggcaat ggctttcagg ttaaggcgta ccagcgtgct
     9721 gccaccaccc gccagcggca gtgagttgta acagctcaca atcccgtagc ccccttttgt
     9781 gaaaatttta tcatgcaccg gaccgttggc gatgtgcggt ttgctacatt cacagatgtt
     9841 cttcgccact tccagcagca ggtcatcagg ggggatttca ggatcgtaga taaaggtcag
     9901 gttcggtgaa acctgcttca actctgcgtc tgcacgtaag atcgcgcggg taatgggcga
     9961 atcagacggg ccgatattgg cgtgcataaa ggcgtctggc agggttccgt cgaggtaacg
    10021 ccagaaacgt tttattcgaa catcgatctc gtcttgtgtt agaattctaa catacggttg
    10081 caacaacgca tccagttgcc ctaggtagac cggcatcgat gtgaccgacg gtacgtggtg
    10141 gtaaagaatg gtcagcagag agagtgcgtc atcaagatct ttcgcgcctt ccagctccag
    10201 ccatt
//
LOCUS       DADLFA010000149        10204 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000149 DADLFA010000000
VERSION     DADLFA010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10204
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.151"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            21..662
                     /locus_tag="JQH65_003158"
     CDS             21..662
                     /locus_tag="JQH65_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004129194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HAY6140484.1"
                     /translation="MRGTNKVTGQSILQRHIASFSHHGLPQGSDDALRYIAEHDDALA
                     FACINRQMIALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVI
                     IPGSRGDYSWIVQPVANEKTLHSLAHGAGRKWGRTECKWRLAAKYTVTQLSRTELGSR
                     IICRDKQLIFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            659..1273
                     /locus_tag="JQH65_003159"
     CDS             659..1273
                     /locus_tag="JQH65_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HAY6140485.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1330..2787)
                     /gene="pepD"
                     /locus_tag="JQH65_003160"
     CDS             complement(1330..2787)
                     /gene="pepD"
                     /locus_tag="JQH65_003160"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008783788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HAY6140486.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNSNKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3048..3506
                     /gene="gpt"
                     /locus_tag="JQH65_003161"
     CDS             3048..3506
                     /gene="gpt"
                     /locus_tag="JQH65_003161"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAY6140487.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3598..4842
                     /gene="frsA"
                     /locus_tag="JQH65_003162"
     CDS             3598..4842
                     /gene="frsA"
                     /locus_tag="JQH65_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HAY6140488.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIVMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4900..5301
                     /locus_tag="JQH65_003163"
     CDS             4900..5301
                     /locus_tag="JQH65_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HAY6140489.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5340..6395)
                     /gene="phoE"
                     /locus_tag="JQH65_003164"
     CDS             complement(5340..6395)
                     /gene="phoE"
                     /locus_tag="JQH65_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HAY6140490.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGGEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAIGMTYQF"
     gene            6683..7786
                     /gene="proB"
                     /locus_tag="JQH65_003165"
     CDS             6683..7786
                     /gene="proB"
                     /locus_tag="JQH65_003165"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAY6140491.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7798..9051
                     /gene="proA"
                     /locus_tag="JQH65_003166"
     CDS             7798..9051
                     /gene="proA"
                     /locus_tag="JQH65_003166"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAY6140492.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTCDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9166..9241
                     /locus_tag="JQH65_003167"
     tRNA            9166..9241
                     /locus_tag="JQH65_003167"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9199..9201,aa:Thr,seq:cgt)
     gene            complement(9256..>10204)
                     /locus_tag="JQH65_003168"
     CDS             complement(9256..>10204)
                     /locus_tag="JQH65_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY6140493.1"
                     /translation="KKSLDDDKSRIGFWLQHFAGMQPRDITESKIYSAMQKMTNRRHE
                     ENWKLRAEACRKKGKPVPEYTPKPASVATKATHLSFIKALLRAAEREWKMLDKAPIIK
                     VPQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSNIINLEWQQIDMQRR
                     VAWINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKESCTKPDGTKAPTVRK
                     MRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQEMGGWESIEMVRR
                     YAHLAPNHLTEHARQIDSILNPSVPNLSQSRNKERTNDV"
BASE COUNT         2446 a         2509 c         2661 g         2588 t
ORIGIN      
        1 tggctggcta acaccccatt ataaggggta cgaataaagt aacggggcag tctattttac
       61 agcggcatat tgcctcgttc tcgcatcatg gtttgcctca aggcagtgac gacgcgctac
      121 ggtatattgc ggaacatgac gatgcgctgg cgtttgcgtg tattaatcgc cagatgatcg
      181 ctttgcgcat aatgcaacaa gttaaggcca cgggaagtcc ggttctggat gtggcgcata
      241 actttgttag cgcgtgtcaa atcggcgatc aacagggctg gttgcatcgt aaaggtgcca
      301 cgccggatga caacggtctg gtgattattc ccggttcacg tggtgattac tcctggattg
      361 tacagcctgt tgcgaacgaa aaaacgctcc actcgctggc gcatggggca gggcgtaaat
      421 gggggcgcac cgagtgtaaa tggcgtctgg cagcgaaata cacggtgacg caactctcgc
      481 ggactgaact tggcagtcgg ataatttgtc gcgataaaca actcatcttt gaagaagcgc
      541 cacaagccta taaatcggct gaaagcgtgg tgcaatgtct ggtgcaggcc ggtttaatta
      601 ttcctgtcgc gcggctgcgt ccggtgctaa cgctcaaaaa cagtggaggg aaaaaaggat
      661 gatcttgcta caactctcct cagcgcaggg gccggaagaa tgttgtctcg cagtgaaaaa
      721 ggcgctggac aggctaatta aagaagctgc ccgacaagac gtcgcggtaa cggtgttgga
      781 aacagaaacg ggccgctact ctgacacgct gcgttcagct ctggtttctc tggatggcga
      841 taacgcatgg gcattaagcg aaagctggtg tggcactatt cagtggattt gtccgagtcc
      901 gtatcggcct catcatgggc gcaaaaactg gtttctgggc attgggcgtt ttaccgctga
      961 tgagcaggag caatccgatg cgatccggta tgagacgctg cgttcgtcgg ggccgggcgg
     1021 tcaacatgtc aataaaaccg actcggcggt acgcgccacg catttggcat ccggtattag
     1081 cgtgaaggtt cagtcagagc gtagtcagca tgctaacaag cggctggcac gattgctgat
     1141 tgcctggaag ctggagcagc agcaacagga aaatagcgcg gtgctgaaat cgcagcggcg
     1201 aatgttccat caccagattg aacgtggcaa cccgagacgg acatttacag ggatggcttt
     1261 tatcgaagga taatgaagga tgaaactgcc ggaaggcgat taaacgccat ccggcagcaa
     1321 atcaaataat tacttcgccg gaatttcttt cagcagttca gtcagcagtg tccagtaatg
     1381 acctacgctt tcgatgtgaa cttgctcatc cggagagtgt ggaccggtga tagttggccc
     1441 gatagaaacc atgtccattt ccggatacgg ttttttgaac agaccacatt ccaggcccgc
     1501 gtggataatc tggatgttcg gcgtcttgtt gaacaggcgc tgataggttt cacgtaccag
     1561 atgcatcacc ggagaattag cgtccggctg ccagccagga tatgcgcctt tcgcttcggt
     1621 tttcgcgcca gccagtttac ccagcgaatc cagcatgctc accacgtagt ctttaccgct
     1681 gtcgatcagt gaacggatca ggcagtgaat ttctacgtta ttgtcagtca tggtcaccac
     1741 accgacgttc agggaggttt caaccacacc tttggctaca tcggagttgc ggatcacacc
     1801 gttcggggtg gcgttcagca gacgaataaa ggtatcgcga gatttcgcaa tcagggcagc
     1861 tttatcgttc gctacagagt ccagcaacaa ggccagattt ttctctttct ctgccagctc
     1921 gtttttcagg atctcctgat aggtattcac cagagatttc agtgcgtcga ctttatcagc
     1981 tgcgacagca atggtcgcaa aggcttcacg cgggatggcg ttacgcagtg tgccgccgtt
     2041 gaaatcgata aggcgcaggt ccagttcttc tgcatgaccc gccaggaagc gcaccagcag
     2101 tttgttggaa ttacccaggc caacgtggat ttcaccgccg gagtgaccgc ctttcagacc
     2161 ttttaaggtt aacttgaagg tttcaaaacc agctggaacc gcttcacgat ctaaatgcag
     2221 gttggaggtg aagtcgatac cccccgcaca acccatgtag atttcacctt cttcttcgga
     2281 gtcggtgtta atcagaatat cagcctgcaa ccagttgctc tgtaagccaa aagcaccgtc
     2341 cataccggct tcttcggtca tggtcagcag cacttccagc ggaccgtgaa ccacgttttc
     2401 gtcagccaga accgccagtg cagaggccat accaatgccg ttatccgcac ccagcgtggt
     2461 gccgcgcgct ttaacccatt cgccatcaat ataaggctgg ataggatctt tcgtgaagtc
     2521 atgcacggtg tcgttatttt tctgcggcac catatcgagg tgggcctgta agacgaccgg
     2581 tttacgattt tccatacctg cggtagcagg tttacgaatc aggatattac ctacctgatc
     2641 gcgttcgaca tggaaacctt tctcttttgc ccaaccaaca atgtattcag cgagttgctc
     2701 ttcatgatag gacgggtgcg gaatagaaca gattttggca aaaatatccc acagcggctg
     2761 tggagataat tgagacagtt cagacacgtt aagtctcctt gtcgatcacc cgcaaaacag
     2821 tattgcaggt cacagggtta gcagaaaatg ttgtcaacac aagacaggct tgcgagatat
     2881 gtttgagaat accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact
     2941 catgtgaaat actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc
     3001 cctcgaacac tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat
     3061 acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc
     3121 cttctgaaca atggaaaggc attattgccg taagccgtgg cggtctggta ccgggtgcgt
     3181 tactggcgcg tgaactgggt attcgtcatg tcgataccgt ttgtatttcc agctacgatc
     3241 acgacaacca gcgagagctt aaagtgctga aacgcgcaga aggcgatggc gaaggcttca
     3301 tagttattga tgacctggtg gataccggtg gtactgctgt tgcgattcgt gaaatgtatc
     3361 caaaagcgca ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg gttgatgact
     3421 atgttgttga tattccgcaa gatacctgga tcgaacagcc gtgggatatg ggcgtcgtat
     3481 tcgtcccgcc aatctccggt cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc
     3541 tttttaactt caggcaggtt acaatagttt ccagtaagta ttctggaggc tgcatccatg
     3601 acacaggcaa acctgagcga aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg
     3661 acgctagtcc gccgctttaa tcacggcgca caaccgcctg tgcagtcggc tcttgatggt
     3721 aaaaccatcc ctcactggta tcgcatgatt aaccgtctga tgtggatctg gcgcggtatt
     3781 gacccacgtg aaatcctcga cgtccaggca cgtattgtga tgagcgatgc tgaacgtacc
     3841 gacgatgatt tatacgatac tgtgattggc taccgtggtg gcaactggat ttatgagtgg
     3901 gccacccagg cgatggtgtg gcaacaaaaa gcctgtgcgg aagaagatcc gcaactcagt
     3961 ggtcgacact ggctgcatgc ggctacgttg tacaacattg ccgcctatcc tcatctgaaa
     4021 ggagatgacc tggccgagca agcgcaggct ttatcaaacc gcgcctatga agaggccgcc
     4081 cagcgtctgc cgggcacgat gcggcagatg gagtttaccg tacccggcgg tgcgcccatc
     4141 accggctttt tgcatatgcc gaaaggcgat ggcccgttcc cgacagtatt aatgtgtggt
     4201 ggtctggatg cgatgcagac ggactattac agcctgtatg aacgttattt tgcgccgcgc
     4261 ggcattgtga tgctgactat tgatatgccg tcggtgggct tttcttcaaa atggaagctc
     4321 acccaggact ccagcctgtt gcatcagcac gtcttaaagg cgctgcctaa cgtaccgtgg
     4381 gtggatcaca ctcgcgtcgc ggcctttggt ttccgtttcg gcgctaacgt tgccgtgcgt
     4441 ctggcatatc ttgaatcgcc gcgtctgaaa gcggttgcct gtcttggtcc ggtagttcat
     4501 accctgttga gtgattttaa gtgccagcaa caggtgccgg aaatgtatct tgacgttctg
     4561 gcgagtcgtt tggggatgca tgatgcttcc gacgaagcgt tacgcgtgga gctgaatcgc
     4621 tattcattaa aagtgcaagg attgctggga cgtcgctgcc caacgccaat gttatcaggc
     4681 tactggaaga acgatccgtt cagcccggaa gaggactcac gcttaatcac ctcatcatct
     4741 gctgacggta aattattaga gatcccattt aacccggtgt atcggaattt tgacaaaggt
     4801 cttcaggaaa tcaccggctg gatcgaaaaa cgcttgtgtt aaaaatttgc taaattttgc
     4861 caatttggta aaacagttgc atcacaacag gagatagcaa tgacgttacc gagtggacac
     4921 ccgaagagca gattgatcaa aaaatttacc gcactaggcc cgtatattcg tgaaggtaag
     4981 tgcgaagata atcgattctt tttcgattgt ctggctgtat gcgtcaacgt gaaaccggca
     5041 ccggaagtgc gtgaattctg gggctggtgg atggagctgg aagcgcagga atcccgtttt
     5101 acctacagct accagtttgg tctgttcgat aaagcaggcg actggaagag tgttccggta
     5161 aaagatactg aagtggttga acgactggag cacaccctgc gtgagtttca cgagaagctg
     5221 cgtgaactgc tgacgacgct gaatctgaag ctggaaccgg cggatgattt tcgtgacgag
     5281 ccggtgaagt taacggcgtg agtgaaatgt gccggatgca tcacatccgg caatatctat
     5341 taaaactgat acgtcatccc aatcgcgaca atatcatcat tattaatatt caatttgtta
     5401 tcgctatcca gttggttgat tttataatca acaaatgctg acatattttt gttgaaataa
     5461 tacgtagcac cgacgtcgat ataattaacc agatcttcac caccgatgcc ctcaatatct
     5521 ttccctttcg ataagacata acccagcgat ggacgcagac caaagtcaaa ctggtattga
     5581 gcgaccgctt caaagttctg tgtcttattg gcaaagccgc cagttattgg cgtcattttg
     5641 cgagtttcag aatagaaagt tgccagataa atattattgg catcgtattt cagacctgtt
     5701 gcccatgctt ctgcacgctt gcctgtgcca cggctttgca ggttctgctc gttggtgcga
     5761 tctgagttgg tataggcccc actaatggcg aaatcgctgc cgccaaagtc atatgtcaat
     5821 gacgtgccga agccatcgcc gttttgcttt ttaacgtcgc ggttttcgtt tttcccttga
     5881 tattgcaggg ttaagttcag gccatcgata acgccgaaga agtcggtgtt ccgatacgtc
     5941 gccagaccgc tggcgcgttt ggtcataaag ttgtcggtct gcgccgagga gtcgccacca
     6001 aattccggaa acatatcggt ccaggcttcc acgtcataca acgcgcccag gttacgacca
     6061 taatcgaaag aacccaaatc tttatatttc aacccggcaa aagcgagacg cgttttttgc
     6121 tgtgcagtat cactctctgc tttattaccg gcaaactctg cttcccaacg accataacca
     6181 gtcagttgat cgttaatttg tgtttcgcct ttaaaaccaa aacggatata actctggtcg
     6241 ccatctttac tgtcgttatc actcatataa tgcatggctt taactttgcc atagacatcc
     6301 agtttattac cgtctttgtt atatatttct gcggcctgta cggatgcaga tgccacaatg
     6361 cccatcacca ctaatgccag agtgctcttt ttcattttca ttcctgattt taattaacgc
     6421 gcgaatattc agcgggagag ccccgttgaa aacaggaaag tttttaacct gagattgtta
     6481 aagatatatt acagattaat aatattctta aaatgtggta atttattaaa tctgtaataa
     6541 aagcgtaaac aactgccgct aggcttgctg atcccgcgca acaaaacgcc atgctttgct
     6601 cgcagatggt tggcaaccga cgacagtcct gctaaaacgt tcgtttgata tcatttttcc
     6661 taaaattgaa tggcagagaa tcatgagtga cagccagacg ctggtggtaa aactcggcac
     6721 cagtgtgcta acaggcggat cgcgccgtct gaaccgtgcc catatcgttg aacttgttcg
     6781 ccagtgcgcg cagttacatg ccgccgggca tcggattgtt attgtgacgt cgggcgcgat
     6841 cgccgccgga cgtgagcact tgggttaccc ggaactgcca gcgactatcg cctcgaaaca
     6901 actgctggca gcggtagggc agagtcgact gattcaactg tgggaacagc tgttttcgat
     6961 ttatggcatt cacgtcgggc aaatgctgct gacccgtgct gatatggaag accgtgaacg
     7021 cttcctgaac gcccgcgaca ccctgcgtgc gttgctcgat aacaatatcg ttccggtaat
     7081 caatgagaac gatgctgtcg ctacggcaga gattaaagtc ggcgataacg ataacctttc
     7141 tgcgctggcg gcgattctgg cgggtgccga taaactgttg ctgctgaccg atcaaaaagg
     7201 tttgtatacc gctgacccgc gcagcaatcc gcaggcagaa ctgattaaag atgtttacgg
     7261 cattgatgac gcactgcgcg cgattgccgg tgacagcgtt tcaggcctcg gaactggcgg
     7321 catgagtacc aaattgcagg ccgctgacgt ggcttgccgt gcgggtatcg acaccattat
     7381 tgccgcgggc agcaagccgg gcgttattgg tgatgtgatg gaaggcattt ccgtcggtac
     7441 gctgttccat gcccaggcga ctccgcttga aaaccgtaaa cgctggattt tcggtgcgcc
     7501 gccggcgggt gaaatcacgg tagatgaagg ggcaactgcc gccattctgg aacgcggcag
     7561 ctccctgttg ccgaaaggca ttaaaagcgt gactggcaat ttctcgcgtg gtgaagtcat
     7621 ccgcatttgc aacctcgaag gtcgcgatat cgcccacggt gtcagtcgtt acaacagcga
     7681 tgcattacgc cgtattgccg gacaccactc gcaagaaatt gatgcaatac tgggatatga
     7741 atacggcccg gttgccgttc accgtgatga catgattacc cgttaaggag ctggccgatg
     7801 ctggaacaaa tgggcattgc cgcgaagcaa gcctcgtata aattagcgca actctccagc
     7861 cgcgaaaaaa atcgcgtgct ggaaaaaatc gccgatgaac tggaagcaca aagcgaaatc
     7921 attctcaacg ctaacgccca ggatgttgct gacgcgcgag ccaatggcct tagcgaagcg
     7981 atgcttgacc gtctggcact gacgcccgca cggctgaaag gcattgccga cgatgtacgt
     8041 caggtgtgca acctcgccga tccggtgggg caggtaatcg atggcggcgt actggacagc
     8101 ggcctgcgtc ttgagcgtcg tcgcgtaccg ctgggagtta ttggcgtgat ttatgaagcg
     8161 cgcccgaacg tgacggttga tgtcgcttcg ctgtgcctga aaaccggtaa tgcggtgatc
     8221 ctgcgcggtg gcaaagaaac gtgtcgcact aacgctgcaa cggtggcggt gattcaggac
     8281 gccctgaaat cctgcggctt accggcgggt gccgtgcagg cgattgataa tcctgaccgt
     8341 gcgctggtca gtgaaatgct gcgtatggat aaatacatcg acatgctgat cccgcgtggt
     8401 ggcgctggtt tgcataaact gtgccgtgaa cagtcgacaa tcccggtgat cacaggtggt
     8461 ataggcgtat gccatattta cgttgatgaa agtgtagaga tcgctgaagc attaaaagtg
     8521 atcgtcaacg cgaaaactca gcgtccgagc acatgtaata cggttgaaac gttgctggtg
     8581 aataaaaaca tcgccgatag cttcctgccc gcattaagca aacaaatggc ggaaagcggc
     8641 gtgacattac acgcagatgc agctgcactg gcgcagttgc aggcaggccc tgcgaaggtg
     8701 gttgctgtta aagccgaaga gtatgacgat gagtttctgt cattagattt gaacgtcaaa
     8761 atcgtcagcg atcttgacga tgccatcgcc catattcgtg aacacggcac acaacactcc
     8821 gatgcgatcc tgacctgcga tatgcgcaac gcccagcgtt ttgttaacga agtggattcg
     8881 tccgctgttt acgttaacgc ctctacgcgt tttaccgacg gcggccagtt tggtctgggt
     8941 gcggaagtgg cggtaagcac acaaaaactc cacgcgcgtg gcccaatggg gctggaagca
     9001 ctgaccactt acaagtggat cggcattggt gattacacca ttcgtgcgta aataaaaccg
     9061 ggtgatgcaa aagtagccat ttgattcaca aggccattga cgcatcgccc ggttagtttt
     9121 aaccttgtcc accgtgattc acgttcgtga acatgtcctt tcagggccga tatagctcag
     9181 ttggtagagc agcgcattcg taatgcgaag gtcgtaggtt cgactcctat tatcggcacc
     9241 atttaaatca ataagttaca catcattagt acgttcctta tttcttgact gggacaaatt
     9301 tgggaccgat gggttcagaa tcgagtctat ttgccgtgcg tgttcggtaa ggtgattagg
     9361 tgcgaggtga gcatatcgac gaaccatttc gatagactcc cagcctccca tttcctgtaa
     9421 cactgataac gggactccgg cttgaaccag ccaacttgcc caggtgtgtc tcaagtcgtg
     9481 aaatctgaaa tcatcaatac ctgctcgtct cagcgccgct ttccaggctg tgtttgcgtc
     9541 ataccgcatc ttcctgactg ttggcgcttt cgttccgtct ggtttggtac agctttcctt
     9601 atacacaaat acccaacggt gatgattccc gatttgtttt ttcaatacgc gacatgcagt
     9661 atcattcagc gcaacgccaa ttgcgcggtt tgatttactc tcttccgggt ttatccatgc
     9721 cacccggcgc tgcatgtcta tttgttgcca ttcaaggttg atgatgttcg agcgtcttaa
     9781 gcctgttgca agtgcaaatt caacaacaga ctttaatggc tccggacatt catcaatcag
     9841 cctttgtgct tcatgaggct ccagccagcg gatccgttta ttctttggtt gaggcacttt
     9901 aataattggt gccttatcca gcattttcca ttcacgctct gcggctctta gcagggcctt
     9961 tataaatgaa agatgcgtag ccttcgttgc aacggacgct ggttttggcg tgtattctgg
    10021 aacaggtttc cctttttttc tgcatgcttc tgccctgagt ttccagtttt cctcatgacg
    10081 ccggttcgtc attttctgca ttgctgaata aatttttgat tcagtaatgt ctcttggttg
    10141 cattcctgcg aaatgttgaa gccagaatcc gatccggctt ttgtcatcgt ccagtgattt
    10201 ttta
//
LOCUS       DADLFA010000150        10180 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000150 DADLFA010000000
VERSION     DADLFA010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10180
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.152"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(497..859)
                     /locus_tag="JQH65_003169"
     CDS             complement(497..859)
                     /locus_tag="JQH65_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HAY6140494.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
     gene            1144..1353
                     /locus_tag="JQH65_003170"
     CDS             1144..1353
                     /locus_tag="JQH65_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6140495.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            complement(1365..1952)
                     /gene="mtlR"
                     /locus_tag="JQH65_003171"
     CDS             complement(1365..1952)
                     /gene="mtlR"
                     /locus_tag="JQH65_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor MtlR"
                     /protein_id="HAY6140496.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(1952..3100)
                     /gene="mtlD"
                     /locus_tag="JQH65_003172"
     CDS             complement(1952..3100)
                     /gene="mtlD"
                     /locus_tag="JQH65_003172"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAY6140497.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPSMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            complement(3330..5243)
                     /locus_tag="JQH65_003173"
     CDS             complement(3330..5243)
                     /locus_tag="JQH65_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAY6140498.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLT
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            5780..6142
                     /locus_tag="JQH65_003174"
     CDS             5780..6142
                     /locus_tag="JQH65_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HAY6140499.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIASLSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            6145..7281
                     /locus_tag="JQH65_003175"
     CDS             6145..7281
                     /locus_tag="JQH65_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY6140500.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGGLQSLTVVPGTDGVQGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(7741..8178)
                     /locus_tag="JQH65_003176"
     CDS             complement(7741..8178)
                     /locus_tag="JQH65_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6140501.1"
                     /translation="MADKAILWALISASTKEERKACSLSYFACKAAEAELGLAYMAAN
                     DNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGKIIRPYLKNLNPLQLAADCIETVNK
                     IKDKNKKIIDINSVNICSDDKNIKLRVNSTIMAIDDSIKCIDE"
     gene            complement(8886..9347)
                     /locus_tag="JQH65_003177"
     CDS             complement(8886..9347)
                     /locus_tag="JQH65_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAY6140502.1"
                     /translation="MKACLLLFFYFSLIDQLHGADVKIKENESVMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSEASFRLYQYYCFTKNNIDKQLRFLERSASQGNVTAQFNYGVFLSD
                     TNPTLSEYYNLNRAIYWMEFAVNNGNIDAKSKLQELKKLKRMDRRKNKENP"
     gene            complement(9359..10156)
                     /locus_tag="JQH65_003178"
     CDS             complement(9359..10156)
                     /locus_tag="JQH65_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001582091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HAY6140503.1"
                     /translation="MYHCDHRGLPLALISTEGATAWCAEYDEWSNLLSDENPHHLQQL
                     IRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNFYQYPLNPVINVDPQG
                     LVDINLYPESDLIHSVADEINIPGVFTIGGHGTPTSIESATRSIMTAKDLAYLIKFDG
                     NYKDGMTVWLFSCNTSKGQNSFASQLAKELHTNVIGPDTLWTWWGRGTNGKLKMDTVL
                     TAPTNLNSNKDLMAITTKDLGNWITYGPSGHPISNMQGTPEKPSDIR"
BASE COUNT         2608 a         2410 c         2318 g         2844 t
ORIGIN      
        1 tgtccattgt attattggtg atttatgttg ctttacatta atgacagtga tgtgtgtcga
       61 ttaaatattg ttttaagcat tcatcataaa aatgtatcat tcgtttcaaa agatgtctgt
      121 gtttgtatga atatattatt ttcgtggatt ctaatgatga ttattaataa atgtcaaata
      181 tgaaagaaac catgtctggg aaaaaattta cagataattt attgtatgaa tatgatgatt
      241 aaaatttcac aattaaatcc gaaaaaaaga gacgtaactg cttgttaatt aataaatgat
      301 atcttctata tatttttatt gtttataggt ggctatttcg gtaaaaatgc attttcagaa
      361 tatcaaccag cggtaaatcg acgtaaaggg agtgaattgt ttggtaatta ggcgagtaga
      421 caggagagtc cccgcattga ccagctaagg caagccagat gcgggttgat atgatcgaac
      481 cagtcgtctg aggcgcttaa aacgcagtct tagagaatcc agtcatctcc tgcaggccca
      541 tttcacggcc cagcgcggtc attgggtgca caaccaccag tccgcgaaca cttttcttca
      601 gcttgcccat atcagcctgc tcttttttgg taattgcgcg ctgaaacggc atcttcatca
      661 gcttctgtgc ttctttactc agtttttggc tatgaacctc acgcaggcga gcaatttctg
      721 cttccagcgt ggctttctct ttttccagct cggcatattt gtctgcggct tcaaccagcg
      781 acagatcggc ctgctggtgg cggatggcgt ccaggcgatc gctgagacgt ttgatttcgt
      841 ttttttcgac ttctttcatg tgatggcttc tgataaagat aaatttatag cccctagtat
      901 gcgcgaccag gtgctttgtt cacaactgat gacattgtaa tagttctgca acgtaatcat
      961 taacatgctc aaaaactacg caattcgttg attaattaag aaatctatag aaaaaagata
     1021 ataaacagca gcggatgatt attgaggatt cagcacgttt tccttaatgt catctttacc
     1081 gggaacctaa actaaaattg gtggccgcgc attcccggcg cggggtaata cggagatatc
     1141 atcatgggca aattaggtga aaacgttccg cttcttatcg ataaagccgt agatttcatg
     1201 gcatcaagcc aggcgttccg ggagtatctg aaaaaacttc ctccccgtaa cgcgattccg
     1261 tccggaatac ccgatgaaag cgtgccgtta tatctacaac gtctggagta ttatcgtcgg
     1321 ctttatcggc cgaagcaggt agaggggcag taagcagacg ttgcttactt ttgaaaggct
     1381 ttttttaagc tgattttgga aatcagctca gtcagtgaaa ggaccattgt tgatcgcaca
     1441 gcctgttgat agcgctgaat ttgcattgca tacaaactgg agtctgctgg ttcaaactgt
     1501 ggcggcggtg gtaacgccgc cacgcagtgc agttcaccaa agggtccaag gatttcgtcg
     1561 tcggtaaagg cgtactcgtt gccgtcgtga tttagctctt cacgcaatgc catcagcagt
     1621 tccgcatctt cgtattcctg gcggttaatg acgcccaacc cgtaaatgag ttttaaacgc
     1681 acagaaagat cgcccagcgg accgtcgccg tcgagtaacg gttctacagc atacttcacc
     1741 gcgtaatcgt ctttgcggaa tacctgaagc accagaagat ttaccgcctc ggtcaaaagc
     1801 tcgacggcgg tgatcagaaa gcttcgcacg gttttgccag cattcagacg ctcaagcaca
     1861 cggttttcaa aggcctgggt ttgttccatt gttgcctgca tatctgacaa tctgttattc
     1921 gggcgcaggg tgtcctgcgc ctggtccacc attattgcat tgctttataa gcggttaccg
     1981 cctcggatac aacctcactg ttggcatcaa gaccggaaat ctgtgccagc gccgcctgcg
     2041 gacctttgtc agcgatcagt gctgccagtt cctgagcctg cggatcatct tcactgcgga
     2101 agtgcattgc accagcaatc ccctgaatca gattcttgtg cggaaggctg tattccagcg
     2161 taccgagcag tggcttgatc agacggtcgc cagcactcag tttacgcagc ggctgacggc
     2221 ctacgcgctc tacatcatct ttcagatacg ggttctcaaa acgaccgagg attttctgga
     2281 tgtacgccgc atgtttgtct gcgtcaaagc cgtagcgctt gatcagtacc gcaccacttt
     2341 cttccatcgc accttttacc accgcgcgga ttttctcgtc gagaatagcg tcacgaatgg
     2401 tctgatgacc ggccagtttt ccgaggtacg cggttatagc atgacccgtg ttcagggtga
     2461 agagtttacg ttcgacaaat gccatcaggt tgtcggttaa ctccatgctt gggatgttcg
     2521 gcagtgcgcc tttgaactgc gttttatcga caatccattc gctgaaagtt tctaccgtca
     2581 cttccagcgg atcgttagtt gccgaagccg aaggcggtac gatgcggtca acggcggaat
     2641 cgacaaagcc aacgtgttct tctacccacg ctttggcgtc ttccggcagg gcgttcatca
     2701 catggccttt cagctgcgtg gtaccgcgta ccatgttttc acaggcgatg atgttcagcg
     2761 gggattcatt accttgttct ttgcgtttca ccagcccttt ggcgatagcc ggagcaatac
     2821 gttccagcac aaccgggcca acggcggtag tgactaaatc gacctgagca atcagatcaa
     2881 cgacatcatc accaatgctg ctgacagcat tgacgccgga aacggtatct acctgctcgg
     2941 tttcaccaac cacatgaacc tgatagctat gacgggcatt cagggcatca agtaccacct
     3001 gattgacatc ggcaaacgtc agttggatac ccgcgtctgc cagcagttta ccgataaagc
     3061 cacgaccgat attacctgcg ccaaaatgta atgctttcat agtattaacc ttcatcaatg
     3121 tttttacccg agagggctgg ggtgaggctt ttccctcacc caaaccctct ccgtgaggag
     3181 aggggcagcg ccggatgcgg cgtaagcgcc ttatccggcc tacaagaacg tgcaaattca
     3241 acaaatcgtg atgcatcctg taggcctgat aagcgtagcg catcaggcaa ttaaccccac
     3301 cttctccatg tggagagggt gggattggat tacttacgac ctgccaacag ttccagcact
     3361 tcatccacgc tggtggtgtg tgccagacgc tcgatgacgg actcatcatc cagtgcattg
     3421 gtcaggctgg tgataacctg aatgtgctcg ttgttacggg ctgcaatacc aatcaccaga
     3481 cgggcaatgt catcttcctc ttcaccgaag cgcacgcctt ccgggtactg gcagaacacc
     3541 acgcccgttt tcagtacgcg atctttcgct tcaaccgtac cgtgtggcac cgcgatagac
     3601 tcacccagat aagtcggggt cagtttttca cgatccagca tcgcctgaac gtattccggc
     3661 tcaacgtaac cgcctttcac cagctgctcg ccagcaaaac gaatcgcttc ttctttggtt
     3721 gctgctttgc gaccgaggaa gatgttctcc gcgcccagtt tgaacaggtt agcactggaa
     3781 tcgtcaaagc tgtctttcag actgtctttc actttcactt cgttttcagt atggcgctgg
     3841 gcagcaacca gacgttcggt taggctggtg tacaggccgc tgtcgaggaa gttggtcagc
     3901 gaaatatgct gtgcctgcgg aacctggcgc atagcgcgtt cggtcaggtc acggtgagtg
     3961 atgacgaggt ccacatctgg cggcaggttg ttgatcgcgc tgttagtgac cgaaatctgc
     4021 gacagacctg catcctgaat tttcttacgc agcacgcctg cgcccatcgc acttgaaccc
     4081 ataccggcat cacaggcaac gatgatttta cgtacgtggc tcaggtcgtt agtcacatcg
     4141 cccgcagaca gcggagatgt gcctttagac tcagctttca tgtcctgcat acgacgagtt
     4201 gctgcttcaa tatcatcttc ttctttcact ttgctggttt tcagcaaaat agcagagaca
     4261 acgaaggaga cagccatcgc cgcacacaca cccgcgatgt tagcgaaata agcccctttt
     4321 ggtgtcatcg ccagtacagc aaggatagaa cccggagatg ccggagaaac cagaccaccg
     4381 cccaggatag tcagcgtgaa cacgccagtc ataccgccga ggatgactgc gaggatcaga
     4441 cgcggattca tcagcacata cgggaagtag atttcgtgga taccacccag gaagtggatg
     4501 attgccgcac cgcccgcaga ctgtttagcg ctaccacgac caaagaacat gtacgccagc
     4561 agcacgccca tacctggacc tgggttagct tcaatcagga agaagattga tttacccagt
     4621 tcatgggact gctgaatacc cagcggcgag aagataccgt ggttaatggc gttgttgagg
     4681 aacaggattt tcgccggttc aacaaagata gacgccagcg gcagcatgtc atggacaacc
     4741 atgaagttaa cgcccgcagc cagcattttg gacagggctt caacaatcgg gccaatgccg
     4801 aggaatgcca gaatagcgag gatcatcccg atgatgcctg cggagaagtt attcaccagc
     4861 atctcaaaac cggatttgat cttaccgtct acccagcggt cgaagtgctt aatgcaccag
     4921 ccgcccagcg gacctgcaat catagaaccg aggaacatcg gcatgtctgc gccgacgata
     4981 acgcccatgg tggtgatggc accgactacg ccgccacgtt cgccgcctac cagcttacca
     5041 ccggtataac cgatcagcag cggcaggaga taagtgatca tcggcccgac cagcttcgcc
     5101 agcgtctcgt tcgttaacca ccctgttgga ataaataacg cggtgatgat accccacgcg
     5161 ataaacgcgc cgatatttgg catcaccatg ttgctgagga aacgaccaaa gctttgcact
     5221 ttgatcttaa tatcggatga cataaaaaca ccccttctta tgtttgctgt cgcgcaggct
     5281 ggaagcccga ggttttttgt taatgtggcg gcagaggtag ccggaccttg tagatactgc
     5341 gaaatctggc actgaatcgg ttaactgtcc agtcgacggc cttttgtgtg atattaatca
     5401 cataaatata ggaggtagag aggtaattga cgtgacgttc atcacaaaac gcttgtgtaa
     5461 aaaagcaaca aggcggcgat tttcgctaaa aatggcattt aaatgtgagt agtatcacat
     5521 ttttgttttg atggtttgtt gaatctttgt gatctgaatc acaagatatt ttcttacggc
     5581 gttcacctga tgtgatctac agcatgttat gccttcgtca tacaatcgtt atgtaactaa
     5641 gcaactcatc acctctaaaa attattcttt ggtgttatgg cgagaatcgc gaacccggat
     5701 tattgtcaaa atatcagcct gactcccttc tggctgccag cccaaaaaca acactgataa
     5761 acgtgaatcc gaggcagata tgtttctaaa ctatttcgcg ttgggagtgc tgatcttcgt
     5821 ctttctggtg attttttatg gaatcatcgc gatacatgac atcccttatc tgattgccaa
     5881 aaagcgcaat cacccccatg ccgacgctat tcatacggcg ggctgggtga gcctgtttac
     5941 tctgcatgtt atctggccgt ttctgtggat ctgggcgacg ctctatcaac cggagcgtgg
     6001 ctggggtatg cagtcacatg ttgcgtcgca ggagaaagcg actgacccgg aaatcgcctc
     6061 actttctgac cgaatttccc ggctggagca tcaactcgcc gccgagaaaa agactgacta
     6121 ttccacgttc ccggagatct aattatggat ctattgattg ttttaactta cgtggcgctg
     6181 gcgtgggcgg tctttaaaat cttccgcatt ccggtaaatc agtggacgct ggcgacggcg
     6241 gcgctgggag gcgtgtttct ggtgagtggt ttgattttgt tgatgaacta caaccaccct
     6301 tacactttta ccgcgcaaaa ggcagtgata gcgatcccta tcacgccaca ggtgacggga
     6361 attgttactg aagtcactga caagaataat cagcttattc aaaagggcga ggtgcttttt
     6421 aagctcgacc cggttcgtta ccaggcgcga gttgacagac ttcaggctga cctgatgacg
     6481 gcgacgcata atataaagac gctgcgtgcg cagctcactg aagcgcaggc caacaccacc
     6541 caggtttcag cggagcgcga ccgtctgttt aaaaattatc aacgttactt gaaaggcagc
     6601 caggcggcgg tgaatccgtt ctcggaacgt gacatcgacg atgcgcggca aaatttcctc
     6661 gcgcaggatg cgctggtgaa aggctcggtg gcggagcagg cgcagatcca gagccagctc
     6721 gacagtatgg ttaacggcga gcaatcgcag attgtgagct taagagcgca acttactgaa
     6781 gcaaaatata accttgagca gactgtcatt cgcgcgccga gcaatggcta cgttactcag
     6841 gtactgatcc gcccaggtac atacgcagct gccttgccgc tgcgtccggt gatggtcttc
     6901 atccccgagc aaaaacggca aattgtcgcc caatttcggc aaaactcgct gttacgtctg
     6961 aaacccggcg atgatgcgga agtggtgttt aacgcgctac ctgggcaggt gtttcacggc
     7021 aaactgacta gtattttacc tgtcgtgcca ggcggttctt atcaggcgca ggggggattg
     7081 caatcattaa cggtcgtgcc cggcacggac ggtgtgcagg gaaccattga actggaccct
     7141 aacgatgata tcgatgcctt acccgacggc atctacgccc aggtggcggt ttactccgac
     7201 catttcagcc atgtttcggt gatgcggaaa gtgctgctaa gaatgaccag ctggatgcat
     7261 tatctttatt tggatcattg agggggaggg gggagattat ggaactttgc tccttgtaag
     7321 gactaattat ttttgcatat tacttcatta gctgcgatga tttttcttta atattcatta
     7381 tgttaataag ttctggatga gtgtgctctc attggatttt ttatccgtct tgttctgccg
     7441 caggatgaga ctatacatcg tcgagatcgc aatgatgata tgagttgttc ctcctgcgac
     7501 ggctctaatc aaaaaagagc aggagtgctt ttccatctat tgcgatgatg gctttattat
     7561 ctgaaggatg acagtcacat ttaaaaaata tttaatctat agatcacaat gtagggtcat
     7621 ggaatatatg tatacaacta ttttcttctt cacttttaat tatcgtattc attataaaac
     7681 taagttaaaa aaactcctgg agagtgagtt attaatatat ttttaaataa aattaagtta
     7741 tcattcatcg atgcatttta tagaatcatc aatggccata atagtactat taactctcaa
     7801 ttttatattt ttatcatcac tacaaatatt aactgaattt atatcaatga tttttttatt
     7861 cttatctttt attttattta ctgtttcaat gcaatcagcg gcaagttgga gtggatttag
     7921 atttttcaga tatggtctta tgatctttcc cttacttaat aaatagcatg tataggattc
     7981 ggaaagtcca gcatctattt tgtacctcat tatattagat aaggatgtga gaaattcctt
     8041 gttatcattc gcagccatat atgccagtcc gagttcagcc tctgcggcct tacaggcaaa
     8101 atagcttagt gaacacgctt ttcgttcctc tttagtactg gctgatatta gtgcccataa
     8161 tattgctttg tctgccaaat ctaactctct ctgaaattgt cttgcagtaa ctgcactcgc
     8221 agaaatagag aaaaaaatac aattgttaat aaccttgcca tattttttta gtccgtaact
     8281 gttaatgtgc tatctggctc tgaaaatatt aactcattta atttttgcat gtcattagat
     8341 aaaccagtta tacaaccctc ggaacagaat cgaggtccta aagacggacc aaaagcgttt
     8401 aaacaactat gtattgaaaa tccattgcga ttataagttc ctgtattaac tgacggtact
     8461 aatcgtatgc cgtcaggttt tctattgctt gctcccgggt cattaacatt ccaactccat
     8521 aggccttgag gtattggcca tctatttata aatgtgtaca acggggatta tttttacatt
     8581 gcatattatt accagaagcg actggaatac taagcacatc ataaaaatta tcatcacatg
     8641 gtatatctga aacacacgaa agcataccta aagttatgct gtataaacag gtggctaatc
     8701 ccaaggggat cgattccaga aatcgggttc aacggatact gataaaaatt ccacctacct
     8761 tccagcccaa tcggagcctg agtgttatac cgcccctgct gcggttaatg ttaccgataa
     8821 tagttatagt acagccctga ttcatcatca tactactgct gcggcagtct gatgaactat
     8881 tgcttttacg gattctcctt attcttcctt ctatccattc gctttagctt tttgagctct
     8941 tgaagtttgc tctttgcatc aatattaccg ttatttacag cgaattccat ccaataaata
     9001 gctctattca aattataata ttctgataat gttggatttg tatctgataa aaagacccca
     9061 taattaaact gcgctgtaac attcccctga gatgctgatc tttccaagaa tcgcaattgt
     9121 ttatcaatat tatttttagt aaagcagtaa tattgatata gtcgaaatga tgcctctgaa
     9181 tcaccatgtt gtgatttata cttcagtttc atcagctttt cttcacttaa atcataggtc
     9241 attgctgtag atcccatcac actttcgttt tcttttattt tcacatcagc accatgcaat
     9301 tgatcaataa gagaaaaata aaaaaatagt aacaagcatg ctttcataca tctacaacct
     9361 atcttatatc actgggtttt tctggcgtac cttgcatatt agaaatgggg tgcccagatg
     9421 gcccatatgt tatccaatta ccaaggtctt ttgttgttat agccattaga tccttatttg
     9481 aattaaggtt cgttggtgct gttagcactg tatccatttt taacttacca ttagttcctc
     9541 gcccccacca cgtccatagc gtgtcaggtc ctattacatt tgtatgtaac tctttagcta
     9601 attggctagc aaatgaattt tgtcctttac ttgtattaca agaaaataac caaactgtca
     9661 tcccatcttt ataattccca tcaaatttaa ttagatatgc tagatcttta gctgtcatga
     9721 tactgcgcgt tgcggattca atagatgtgg gggtaccatg ccccccgatt gtgaaaacgc
     9781 ctgggatatt aatctcatca gctacagaat ggataagatc actttcgggg tataaattta
     9841 tatcaaccaa accttgcgga tctacattta tgaccggatt caacggatac tgataaaaat
     9901 tccatccccc cttcagccca atcggatcct gagtgatata ccgcccctgc agcgggtcat
     9961 aatagcggtg gcggttgtaa tacaggccgg actcctcatc atactgctga cccggcaggc
    10021 gaatgagctg ctgcagatga tgcgggttct catcactcag caggttgctc cattcatcat
    10081 attctgcgca ccacgctgtt gccccttccg tgctgatgag cgccagcggc aggccgcgat
    10141 ggtcgcagtg gtacaggtgg atttttcgcg ccggcgtgta
//
LOCUS       DADLFA010000151        10179 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000151 DADLFA010000000
VERSION     DADLFA010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10179
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.153"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            49..993
                     /locus_tag="JQH65_003179"
     CDS             49..993
                     /locus_tag="JQH65_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY6140504.1"
                     /translation="MVVMIHTTTWYVTNAHSVSPVTWDIANVLNSASRVSVPLFFMIS
                     GYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINMELALKNLLQKPVFYHLW
                     FFFAIAVIYLVSPLIQVKNVGGKMLLVLMAVIGIIANPNTVPQKIDGFEWLPINLYIN
                     GDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFIISRGTLYELQWRGNFADT
                     WYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLGIYGFHALIIHALRTRGI
                     ELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(1035..1472)
                     /locus_tag="JQH65_003180"
     CDS             complement(1035..1472)
                     /locus_tag="JQH65_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HAY6140505.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(1518..1859)
                     /locus_tag="JQH65_003181"
     CDS             complement(1518..1859)
                     /locus_tag="JQH65_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6140506.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(2024..2215)
                     /locus_tag="JQH65_003182"
     CDS             complement(2024..2215)
                     /locus_tag="JQH65_003182"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6140507.1"
                     /translation="MGPALGAARLAQIAANPEKSLIELLPQLPLEQSHLPDAQRYAAY
                     QPRRETFRRLYQQLLPLMA"
     gene            complement(2148..3467)
                     /gene="xylB"
                     /locus_tag="JQH65_003183"
     CDS             complement(2148..3467)
                     /gene="xylB"
                     /locus_tag="JQH65_003183"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005054141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HAY6140508.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHSFCH
                     ALPQRWHLMSVMLSAASCLDWVAKLTGLCNVPALIAAAQQADESAEPVWFLPYLSGER
                     TPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTLIGG
                     GARSEYWRQMLADISGQQLDYRTGGCGASTGRSKAGADCGESREIAH"
     gene            complement(3584..4861)
                     /gene="xylA"
                     /locus_tag="JQH65_003184"
     CDS             complement(3584..4861)
                     /gene="xylA"
                     /locus_tag="JQH65_003184"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HAY6140509.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVSFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGR"
     gene            5227..6198
                     /gene="xylF"
                     /locus_tag="JQH65_003185"
     CDS             5227..6198
                     /gene="xylF"
                     /locus_tag="JQH65_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY6140510.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNGETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALTANNNKIDAVVASNDATAGG
                     AIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAEIAVELGN
                     GQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            6276..7817
                     /gene="xylG"
                     /locus_tag="JQH65_003186"
     CDS             6276..7817
                     /gene="xylG"
                     /locus_tag="JQH65_003186"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HAY6140511.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNSRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            7795..8976
                     /gene="xylH"
                     /locus_tag="JQH65_003187"
     CDS             7795..8976
                     /gene="xylH"
                     /locus_tag="JQH65_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HAY6140512.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            9054..>10179
                     /locus_tag="JQH65_003188"
     CDS             9054..>10179
                     /locus_tag="JQH65_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XylR family transcriptional regulator"
                     /protein_id="HAY6140513.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKA"
BASE COUNT         2663 a         2492 c         2417 g         2607 t
ORIGIN      
        1 cagcccaaaa tttactggat tgataacctg cgagggatag cgtgtttaat ggtggtgatg
       61 attcacacca ctacctggta tgtgaccaat gctcatagtg ttagccccgt cacatgggat
      121 atcgccaatg ttctgaattc tgcctctcgt gtcagcgtgc cgctattttt catgatttcc
      181 ggctatctct tttttggcga acgcagcgcc cagccgcgcc atttcttgcg tatcggctta
      241 tgtctgattt tttatagcgc aatcgcactg ctctacattg cgctgtttac ctccatcaat
      301 atggagttag cgctgaaaaa cctgctgcaa aagccagtgt tttaccactt gtggtttttc
      361 ttcgcgattg cggtgattta tctggtttca ccgctgattc aggtgaagaa cgtcggcgga
      421 aaaatgttgc tggtgctaat ggcggtgatt ggcattatcg ctaacccaaa cacagtgccg
      481 cagaaaattg acggttttga atggctgcca attaacttat atatcaatgg cgatactttt
      541 tactacattc tgtatggcat gttgggccgc gctataggga tgatggacac acagcataaa
      601 gcactgtcgt gggtgagcgc cgcgctgttt gcgacggggg tttttattat ctctcgcggg
      661 acattatatg aattgcagtg gcgcggaaat tttgccgata cctggtatct ttactgtggg
      721 ccgatggttt ttatctgcgc aatcgcgcta ttgactctgg ttaaaaacac gctggatacg
      781 cgtaccattc gcggacttgg cttaatctcc cgccattcat tgggtatata cggattccac
      841 gccttgatta tccatgcgct gcgcacccgg ggaattgagc ttaaaaattg gccaatactg
      901 gatattattt ggatcttttg cgcgacgttg gcagcgagtt tgttactttc tatgctggta
      961 caacgaatcg acagaaacag attagtgagt taagtaaaag cccgttcaca ttggactgac
     1021 cgggcttacg tgaattattc gctgtattct tcctgggtaa cctgtgtttc ttttggcggg
     1081 tttattccgg catcacgaat attcttctgc accgctactg caccaaacaa gcttaagaag
     1141 aaagccagtc cataaaaacc tttttcactg agtaataatg tcgcattcca cagacctacc
     1201 atcagtaatg caacagagat aataaagaca gtcaggcagg tcatataata aatggaagtg
     1261 gtcggtatgc cttcatactt gtcccgaacg gtcttttgat aagacgcagc agaaaacagt
     1321 cctaacacca gtacggcaaa ataataccct ttttcattta actgcatctc tgcattccat
     1381 agccctaaca gataggtaac gataccacca acgagagcta tccatgacac aatactaaag
     1441 gccggtgaat aggttgatat tttgttgtcc atcacagtat tcctttcatt cctgaatgtg
     1501 taagagcttt atgttgctca taccgagcga aacaaacgca tttgaccaaa caagcagaca
     1561 aaaaaggcga tggggtagat caccatttca taagcattga taggtgcatt taaaacaccg
     1621 ataaacaaga gaccgatagt gattaacgca actaactggc agatatgggt aaaaaaatta
     1681 tccagttgcc cgagtttttc tgcgcgaaga tatgcatagt taccaaaagt tgctgtcatt
     1741 aacacgccaa gaaaatagcc ttttccactt agcaatgggc atgagatcca tagcccaacc
     1801 agataaacca gcgccccgac aacaaataat agttttgcca cagtttttga ggttttcata
     1861 acgatctcca tatctaccag cgatacatta cgagtaacca acgaaagaca aaactgaaaa
     1921 atgccattaa caaatgattt tcagaataaa ttcatactaa atattaatta gtgagatata
     1981 tagatgtgaa ttatccccca cccggtcagg caggggataa cgtttacgcc attaatggca
     2041 gaagttgctg atagaggcga cggaacgttt cacgtcgtgg ctgataagcg gcataacgct
     2101 gcgcatctgg tagatgcgac tgttctaacg gtagctgcgg caacaactca atgagcgatt
     2161 tctctggatt cgccgcaatc tgcgccagcc ttgctgcgcc cagtgctggc cccacatccc
     2221 cccgtacggt aatcgagctg ctgaccgctg atatccgcca gcatctgacg ccagtactca
     2281 ctacgcgccc cgccaccaat caacgtaaca ctttgcggtt taatgccgca ggcatgcacg
     2341 acatccatgc catctgccag cgcataaccc acgccttcca gcactgctcg cgccagttca
     2401 ttggggccat gttgatgagt caaaccaaag aaaaccccct tcgcctgggg attattgtgt
     2461 ggtgtacgct cgccggaaag ataaggcaga aaccaaactg gctcggcact ttcatcagcc
     2521 tgttgagctg cagcgattaa agctgggaca ttgcacaggc cggttaattt cgcgacccaa
     2581 tccagacacg atgctgcact cagcattaca gacattaaat gccaacgctg cggtagcgca
     2641 tggcaaaagc tgtgtacagc gctttctggc ttgcttaaga acccttcgct gacagcaaaa
     2701 tagacccccg acgtccccag cgataacatt gcctgattag catcaaccat tcccacacca
     2761 actgcattgt cgccaccgcc tgcgacaact ggcaccgtcg ccatacccca cgctttcgca
     2821 acttcaggta acaaagcacc agtaatttcg ctgccttcgt ataatgcggg catctggtca
     2881 cgagataagt cgcaagcctg cagcatgacg tcactccagt cacgctttgc gacatccagc
     2941 cacatggtgc cagctgcgtc agacatatcg ctggcaaact cccccgtcat acgcagacgc
     3001 aagtaatctt tcggtaataa tactttgtcg atttgacgga atatctccgg ctcatgccgc
     3061 tgaacccata gcaatttagg cgcagtaaat ccgggcatca tcaggttgcc ggtaatcact
     3121 cgtgattgcg gaactctcgc ttccagcaaa gtgcactctt gcgcacagcg cccgtcgttc
     3181 cacaaaatgg cagggcgcaa tacccgttgt tgagcatcca gtaaggttgc tccatgcatc
     3241 tggccggcaa tacccaatgc tttaacgtcc tgcagagaat gctgatcgcc cagagctttc
     3301 attgcgcgat cagttgcctg ccaccactgt tccgggtctt gttccgacca gagtggatgc
     3361 gggcgcgaaa cggtcagctt ttccgtttgc gaagcaacca cctcaccctg ctcgttgagc
     3421 aaaataactt ttacgcccga ggtgccaaga tctatcccga tatacatatc gatcgttcct
     3481 taaaaaaatg cccggtatcg ctaccgataa ccgggccaac ggactgcaca gttagccgtt
     3541 atttgtcgaa cagataatga tttaccagat tttccagttg ctcctagcga ccactctgat
     3601 gcaccggaga caaattatgt tcctgagcat atttggctaa atctgccagt gacatttggc
     3661 ctttcaggat ttgctggccc aattcgctat tccagccgga ataacgctgc gcgatgcgtt
     3721 tatccagctc gccatcttca atcatgcgcg ctgcaatttt cagcgccagt gccatcgtat
     3781 ccatcgcgcc gatatgaccg taaaacagat catatttatc agtactttga cgacgtactt
     3841 tggcatcgaa gttcagacca ccggtggtga aaccgcctgc tttgagaatt tcatacatca
     3901 ccagcgcatt ctcttccaca ctgttcggga actggtcggt gtcccagccc agttgcgcat
     3961 cgccacggtt ggcgtcgaca gaaccgaaca ggccaagcgc aatggcggtg gctatttcat
     4021 gatggaaaga gtgacctgcc agcgtcgcgt ggttagcttc aatgttcagt ttaatctctt
     4081 tttccagacc aaactgtttc aggaagccat agaccgtcgc ggcatcgtaa tcatattgat
     4141 gtttagtcgg ttcttgcggt ttcggttcga taagcaacgt gccctggaag ccgattttat
     4201 gtttatgctc aaccaccatc tgcataaagc ggcccagttg ttcacgctcc tgacgcaagt
     4261 cggtatttaa cagcgtttcg taaccttcac gaccacccca caggacatag ttttcaccgc
     4321 ccaatttatg ggttgcttcc atcgctgtaa caacttgcgt tgccgcccag ctgaagactt
     4381 caggatctgg gttcgtcgcc gcacccgcgc catagcgagg gtttgtaaag cagttagcgg
     4441 ttccccacag cagcttcacg ccgctctctt cttgcttggc tgccaggaca tcaaccattt
     4501 gcgcaaaatt attgatgtac tcttttaacg acgcgccctc aggggaaaca tccacatcgt
     4561 ggaagcaata aaatgacaca tgtaacttgt ggaaaaactc aaatgcgaca tctgctttac
     4621 gcttcgccaa cgccagtgcc tcaccaggct gctgccacgg acgattaaac gcccccacac
     4681 caaacatgtc cgccccgttc cagcagaagg tgtgccagta acaggcggca aaacgcaagt
     4741 gctcttccat acgcttaccc aacaccagtt cgtcgggatt gtagtgacgg aatgctaacg
     4801 ggtttgagga ttttgagcct tcataacgaa cgcgatcgag ctggtcaaaa taggcttgca
     4861 tattgaactc cataatcagg taatgccgcg ggtgatggat gatgtcgtaa tattgggcac
     4921 tccctttcag ttgctcaatt atgttatttc acactgctat tgagataatt cacaagtgtg
     4981 cgctcgctcg caaaataaaa tgggatgatg aaactgggta attcctcgaa gataaaaatg
     5041 caataagtac aattgcgcaa caaaagtaag atctcggtca taaatcaaga aataaaccaa
     5101 aaatcgtaat cgaaagataa aaatctgtaa ttgttttccc ctgtttagtt gctaaaaatt
     5161 ggttacgttt atcgcggtga ttgttactta ttaaagctgt cctctaacta cagaaggccc
     5221 tacaccatga aaataaagaa cattctactc accctttgca cctcacttct gcttaccaac
     5281 gttgcggcac acgccaaaga agtcaaaata ggtatggcga ttgatgatct acgtcttgaa
     5341 cgctggcaaa aagatcgaga tatttttgtg aaaaaggctg aatctctcgg tgcgaaagta
     5401 tttgtacagt ctgcaaatgg caatggagaa acacaaatgt cgcagattga aaacatgatt
     5461 aaccggggtg tcgatgttct tgtcattatt ccgtataacg gccaggtatt aagtaacgtt
     5521 gtaaaagaag ccaaacaaga aggtattaaa gtattagctt acgaccgtat gattaacgat
     5581 gcggatatcg atttttatat ttctttcgat aacgaaaaag tcggtgaact gcaggcaaaa
     5641 gccctggtcg atattgttcc gcaaggtaat tacttcctga tgggcggctc gccggtagat
     5701 aacaacgcca agctgttccg cgccggacaa atgaaagtgt taaaacctta cgttgattcc
     5761 ggaaaaatta aagtcgttgg tgaccaatgg gttgatggct ggttaccgga aaacgcgcta
     5821 accgccaata ataacaaaat tgatgctgta gttgcctcaa acgatgccac cgcaggtggg
     5881 gcaattcagg cattaagcgc gcaaggttta tcagggaaag tagcaatttc cggccaggat
     5941 gcggatctcg caggtattaa acgtattgct gccggtacgc aaactatgac ggtgtataaa
     6001 cctattacat tgctggcaaa tactgccgca gaaattgccg ttgaattggg caatggtcag
     6061 gagccaaaag cagatacctc actgaataat ggcctgaaag atgtcccctc ccgcctcctg
     6121 acaccgatcg atgtgaataa aaacaacatc aaagatacgg taattaaaga cggattccac
     6181 aaagagagcg agctgtaagc gttacgcccc ggcgcggagc gggggcgtga tttctctcca
     6241 tgccgcgtga atgaattggc ttaggtggag tcgttatgcc ttatctactt gaaatgaaga
     6301 acattaccaa aaccttcggc agtgtgaagg cgattgataa cgtcagcttg cggttgaatg
     6361 ctggcgaaat cgtctcactc tgtggggaaa atgggtctgg taaatcaacg ctgatgaaag
     6421 tgctgtgtgg tatttatccc catggctcct acgaaggcga aattattttt gcgggagaag
     6481 agattcaggc gagtcacatc cgcgataccg aacgcaaagg tatcgccatc attcaccagg
     6541 aattggccct ggtgaaagaa ttgaccgtgc tggaaaatat cttcctgggt aacgaaataa
     6601 cccacaatgg cattatggat tatgacctga tgacgctacg ctgtcagaag ctgctcgcac
     6661 aggtcagttt atccatttca cctgataccc gcgttggcga tttagggctt gggcaacaac
     6721 aactggttga aattgccaag gcacttaata aacaggtgcg cttgttaatt ctcgatgaac
     6781 cgacagcctc attaactgag caggaaacgt cggttttact ggatattatt cgcgatctac
     6841 aacagcacgg tatcgcctgt atttatattt cgcacaaact caacgaagtc aaagcgattt
     6901 ccgatacgat ttgcgttatt cgcgacggtc agcacattgg tacgcgtgat gctgccggaa
     6961 tgagtgaaga cgatattatc accatgatgg tcgggcgaga gttaaccgcg ctttacccta
     7021 atgaaccaca taccaccgga gatgaaatat tacgtattga acatctgacg gcatggcatc
     7081 cggttaatcg tcatattaaa cgagttaatg atgtctcgtt ttccctgaaa cgtggcgaaa
     7141 tactgggtat tgccggactc gttggtgccg gacgtaccga gaccattcag tgcctgtttg
     7201 gcgtgtggcc cggacaatgg gaaggaaaaa tttatattga tggcaaacag gtagatattc
     7261 gtaactgtca gcaagccatc gcccagggga ttgcgatggt acccgaagac agaaagcgcg
     7321 acggcatcgt tccggtaatg gcggttggta aaaatattac cctcgccgca ctcaataaat
     7381 ttaccggtgg cattagccag cttgatgacg cggcagagca aaaatgtatt ctggaatcaa
     7441 tccagcaact caaagttaaa acgtcgtccc ccgatcttgc tattggacgt ttgagcggcg
     7501 gcaatcagca aaaagcgatc ctcgcccgct gtctgttact caactcgcgc attctcattc
     7561 ttgatgaacc taccaggggt attgatattg gcgcgaaata tgagatctac aaattaatta
     7621 accaactcgt ccagcagggt attgccgtta ttgtcatctc ttccgaatta cctgaagtgc
     7681 tcggccttag cgatcgtgta ctggtgatgc atgaagggaa actaaaagcc aacctgataa
     7741 atcataacct gactcaggag caggtgatgg aagccgcatt gaggagcgaa catcatgtcg
     7801 aaaagcaatc cgtctgaagt gaaattggcc gtaccgacat ccggtggctt ttccgggctg
     7861 aaatcactga atttgcaggt cttcgtgatg attgcagcta tcatcgcaat catgctgttc
     7921 tttacctgga ccaccgatgg tgcctactta agcgcccgta acgtctccaa cctgttacgc
     7981 cagaccgcga ttaccggcat cctcgcggta ggaatggtgt tcgtcataat ttctgctgaa
     8041 atcgaccttt ccgtcggctc aatgatgggg ctattaggtg gcgtcgcggc gatttgtgac
     8101 gtctggttag gctggccttt gccacttacc atcattgtga cgttggttct gggactgctt
     8161 ctcggtgcgt ggaacggatg gtgggtcgcg taccgtaaag tcccttcatt tattgtcacc
     8221 ctcgcgggca tgttggcatt tcgcggcata ctcattggca tcaccaacgg cacgactgta
     8281 tcccccacca gcgccgcgat gtcacaaatt gggcaaagct atctccccgc cagtaccggc
     8341 ttcatcattg gcgcgcttgg cttaatggct tttgttggtt ggcaatggcg cggaagaatg
     8401 cgccgtcagg ctttgggttt acagtctccg gcctctaccg cagtagtcgg tcgccaggct
     8461 ttaaccgcta tcatcgtatt aggcgcaatc tggctgttga atgattaccg tggcgttccc
     8521 actcctgttc tgctgctgac gttgctgtta ctcggcggaa tgtttatggc aacgcggacg
     8581 gcatttggac gacgcattta tgccatcggc ggcaatctgg aagcagcacg tctctccggg
     8641 attaacgttg aacgcaccaa acttgccgtg ttcgcgatta acggattaat ggtagccatc
     8701 gccggattaa tccttagttc tcgacttggc gctggttcac cttctgcggg aaatatcgcc
     8761 gaactggacg ctattgcagc atgcgtgatt ggcggcacca gcctggctgg cggtgtggga
     8821 agcgttgccg gagcagtaat gggggcattt atcatggctt cactggataa cggcatgagt
     8881 atgatggatg taccgacctt ctggcagtat atcgttaaag gtgcgattct gttgctggca
     8941 gtatggatgg actccgcaac caaacgccgt tcttgatttt gataaaaatt ttctcaaagc
     9001 cggttacgta ttaccggttt tgagtttttg catgattcag caggaaaaga accatgttta
     9061 ctaaacgtca ccgcatcaca ttactgttca atgccaataa agcctatgac cggcaggtag
     9121 tagaaggcgt aggggaatat ttacaggcgt cacaatcaga atgggatatt ttcattgaag
     9181 aagatttccg cgcccgcatt gataaaatca aggactggtt aggagatggc gtcattgccg
     9241 acttcgacga caaacaaatc gagcaagcgc tggctgatgt cgacgtcccc attgttgggg
     9301 ttggcggctc gtatcacctt gcagaaagtt acccacccgt tcattacatt gccaccgata
     9361 actatgcgct ggttgaaagc gcatttttgc atttaaaaga gaaaggcgtt aaccgctttg
     9421 ctttttatgg tcttccggaa tcaagcggca aacgttgggc cactgaacgc gaatatgcat
     9481 ttcgtcagct tgtcgccgaa gaaaagtatc gcggagtggt ttatcagggg ttagaaaccg
     9541 cgccagagaa ctggcaacac gcgcaaaatc ggctggcaga ctggctacaa acgctgccac
     9601 cgcaaaccgg gattattgcc gttactgacg cccgggcgcg gcatattctg caagtatgtg
     9661 aacatctaca cattcccgta ccggaaaaat tatgcgtgat tggcatcgac aacgaagaac
     9721 tgacccgcta tctgtcgcgt gtcgcccttt cttcggtcgc tcagggcgcg cggcaaatgg
     9781 gctatcaggc ggcaaaactg ttgcatcgat tattagataa agaagaaatg ccgctacagc
     9841 gaattttggt cccaccagtt cgcgtcattg aacggcgctc aacagattat cgctcgctga
     9901 ccgatcccgc cgttattcag gccatgcatt acattcgtaa tcacgcctgt aaagggatta
     9961 aagtggatca ggtactggat gcggtcggga tctcgcgctc caatcttgag aagcgtttta
    10021 aagaagaggt gggtgaaacc atccatgcca tgattcatgc cgagaagctg gagaaagcgc
    10081 gcagtctgct gatttcaacc accttgtcga tcaatgagat atcgcaaatg tgcggttatc
    10141 catcgctgca atatttctac tctgttttta aaaaagcat
//
LOCUS       DADLFA010000152        10146 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000152 DADLFA010000000
VERSION     DADLFA010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10146
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.154"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..236
                     /locus_tag="JQH65_003189"
     CDS             <1..236
                     /locus_tag="JQH65_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY6140514.1"
                     /translation="TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLV
                     YEKTYTAELSSIAGTPIEAGPFDTVVLFKINYN"
     gene            343..1434
                     /locus_tag="JQH65_003190"
     CDS             343..1434
                     /locus_tag="JQH65_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAY6140515.1"
                     /translation="MLYRYTKEKIMRERRMPVLQWGMLCVLSLLLSIGFLAVHLPAAL
                     LLGPMIAGIIFSMRGITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAI
                     LLVTLLSSAIVGWLLVRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYL
                     RVLFVAGAAVLVTCMMLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLP
                     SGTMLIPMLAGAVLQSGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQ
                     ILLSIFALLAICAGMAWGLTRFMHIDFMTAYLATSPDGLDTVAVIAAGSNADMALIMA
                     MQTLRLFSILLTGPAIARFISTYAPKRSA"
     gene            complement(1431..2222)
                     /gene="nei"
                     /locus_tag="JQH65_003191"
     CDS             complement(1431..2222)
                     /gene="nei"
                     /locus_tag="JQH65_003191"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HAY6140516.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVGTRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(2258..2992)
                     /gene="pxpA"
                     /locus_tag="JQH65_003192"
     CDS             complement(2258..2992)
                     /gene="pxpA"
                     /locus_tag="JQH65_003192"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HAY6140517.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(2982..3914)
                     /gene="pxpC"
                     /locus_tag="JQH65_003193"
     CDS             complement(2982..3914)
                     /gene="pxpC"
                     /locus_tag="JQH65_003193"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HAY6140518.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLNVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSPQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(3908..4564)
                     /gene="pxpB"
                     /locus_tag="JQH65_003194"
     CDS             complement(3908..4564)
                     /gene="pxpB"
                     /locus_tag="JQH65_003194"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HAY6140519.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIELLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(4587..5330)
                     /gene="ybgI"
                     /locus_tag="JQH65_003195"
     CDS             complement(4587..5330)
                     /gene="ybgI"
                     /locus_tag="JQH65_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HAY6140520.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            5601..7082
                     /gene="dtpD"
                     /locus_tag="JQH65_003196"
                     /pseudo
     CDS             5601..7082
                     /gene="dtpD"
                     /locus_tag="JQH65_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
     gene            complement(7124..8542)
                     /gene="phrB"
                     /locus_tag="JQH65_003197"
     CDS             complement(7124..8542)
                     /gene="phrB"
                     /locus_tag="JQH65_003197"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HAY6140521.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACSNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVMLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(8539..9048)
                     /locus_tag="JQH65_003198"
     CDS             complement(8539..9048)
                     /locus_tag="JQH65_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HAY6140522.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            complement(9333..9719)
                     /locus_tag="JQH65_003199"
     CDS             complement(9333..9719)
                     /locus_tag="JQH65_003199"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6140523.1"
                     /translation="MWNDIFTKLSIAILLAFSAYVHAETSAPIFFEDVGSIDSWFSIK
                     YQKYENVKDEQYPEILIYYGVKGSGINRVDGWFFSCAKAGRTCSLIFMADFGESRYFK
                     PAKTAMNKNNLIIRTSDVEIAFKITK"
     gene            complement(9692..>10146)
                     /locus_tag="JQH65_003200"
     CDS             complement(9692..>10146)
                     /locus_tag="JQH65_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HAY6140524.1"
                     /translation="LGLIDKPAPVFNRDLNSDPYYLAVNNCYSYALNRYGNPGSRIFG
                     GGGLQPGELSGKEFSKLTCGSIFEASKNDGAKDLDNGSCPSGYHKAQLFIRPHNFIGM
                     GGDYHWYRQDANGEWSDKQGVGAIRFRGKDPFPPIDYPEKCGTIFLPN"
BASE COUNT         2365 a         2753 c         2439 g         2589 t
ORIGIN      
        1 aaacgttggt tgaagaagat aaagcattgt tgattggtaa tggtctgaag ctacgtttgc
       61 tggatgaaaa cgcctcgcct tataccttca ataaatatgc tgaatacgcc gatttcacca
      121 gcgacatgtt ggtctacgaa aaaacctata cagctgaact ttcgtccatt gcaggtaccc
      181 ccatcgaggc tgggcccttc gatacagtgg tgctttttaa aattaactat aactgatttg
      241 ccatgatgcc ctttgcagcc ggaaagggca tctctttcag aaaattgctg attttcctct
      301 tttccttgat tactgttatt ttttcctggc ccggctttct ttatgctgta tcgatacacc
      361 aaagagaaaa taatgaggga gcgaaggatg ccagttttgc agtggggaat gttatgtgtg
      421 ctatcacttc tcctttctat tggtttcctc gcggtacatc tcccggcggc gctattactt
      481 gggccgatga tcgccgggat catcttcagt atgcgcggaa ttactctgca acttccccgc
      541 tccgcttttc tcgccgcgca ggccattctt ggctgcatga ttgcgcaaaa cctcactggt
      601 tcgattctca ccacgctggc cgccaactgg ccgatcgtgc ttgctatttt actggtgacg
      661 ctgctttcca gcgccatcgt gggttggtta ttggtgcgct atagctcact gcctggaaat
      721 accggtgcct ggggttcttc tccaggcggc gcagcggcaa tggtcgctat ggcgcaagat
      781 tacggcgcag atattcgcct ggtggcgttt atgcaatatc tgcgagtgct gttcgtcgcg
      841 ggggccgctg ttctggtcac ctgcatgatg ctgggtgata acgctgaagc ggttaaccag
      901 caaatcgtct ggttcccgcc agtgagcatt aatctcctgc ttacgatttt gctcgcagtc
      961 gttgccggta cggcgggatg catgctgcgt cttccttccg gcacgatgct catcccgatg
     1021 ctggcgggcg cagtgctcca gtctggtcag ctcatcacca tcgaactacc ggaatggctg
     1081 ctggcgatgg cgtatatggc aattggctgg cggattggtc ttggtttcga taaacaaatc
     1141 ttactgcggg cactacgccc actaccgcaa atcctgctgt cgatttttgc tctgctggct
     1201 atttgtgcag gtatggcgtg gggactgacc cggtttatgc atattgattt tatgactgcc
     1261 tacctcgcca ccagtcccga cgggcttgat accgtggcgg tcatcgccgc agggagcaat
     1321 gccgatatgg cgcttattat ggcgatgcaa accctgcgcc tgttcagtat tttgctgacg
     1381 gggccagcca ttgcgcggtt tatttcaacc tatgcgccga agcggtcggc ctagtgctgg
     1441 cagcctggac accagtaaaa cgggcgcgag gacaacgtgg ttttctcaat gatgccgcca
     1501 cagcgttcgc acagttcgcc atcacgatga aaaaccttaa agcgaaacag cgccccatga
     1561 tgcttattct catccacctg cccccgcgta gcgtaagaaa accgaggaat ctccagtaac
     1621 gcgtgtgcga gtgcatccag ttgcgccgca ttgagatctt tcgctttatg atttccagtc
     1681 aacccaacct gccagaggat ctccacccgc aaataattgc caagcccagc cagaaacgcc
     1741 tgatcgagca gtaatcctgc aaactgacgg ttacgaaagc gcggtgacaa taaccgttcc
     1801 tttaccacct ccggcgtcag attcggatcc agcacatcgg gaccaacgcg ttgtaaaaac
     1861 ggatgcgtgg tcagttgttc cggcctcaac atctcaatat ccgaggcgct ataaagcaga
     1921 atggttttgt cagccgtttg cagttttacc cgcaatactc gcgtggtctg cggctcttcg
     1981 ccggtatcaa ccacgcgcca gacgccgtaa agctgattat ggctgtagag cgttaagtcg
     2041 ttggaaaaat gagttaacaa cgccttacca cgcgttccca catgggtaac gtgttgaccg
     2101 ataagttgtg attgataagt ttttaactgc gggaaggcaa accagacatc agttagtggt
     2161 ttgcctttga tcgccgcctc caggttatcc gctgcacggc ggatctccgg gccttcaggc
     2221 atggtgatat ccttttttca ttctttatta atcggggtta tgctgcgaca acaatctcct
     2281 tttcggcaaa tgtagagcgt agtcggcggg cgaacgccag tgcgtgctcg ccgtcgccat
     2341 gcaggcagac tgtttgcgcc gtgaccgttg cccattcgcc ggtgatactt ttgactctgc
     2401 cgtgttgcac catctctagc gtttgcgcca gcgcctgttc ttcgttttca atcaacgcgc
     2461 ctggctgact tcgcggcacc agcgagccgt cagcctgata accgcgatcg gcaaacactt
     2521 cctcgcgcgt tgtcaggcca tattgcttgc ctgcacgaat cagctcgctt cctgccagcc
     2581 caacgagaac caatgctgga tcgcaagcgt ataccgctct ggcgatggcg tctgccagtt
     2641 gtgcttcttt cgccgcctgg ttgtacaaca tgccgtgcgg tttgacatga cgcattacgc
     2701 cgccttgcgc acgggcaatc gttgccagcg cgccaatttg atacagcgtc tgggcgtaaa
     2761 cggtttctgg cggcagctgc atggcgctgc gaccaaaatt ttccctgtcg ggaaaactcg
     2821 ggtgagcgcc aatcgcgaca ccatttttta ttgcttcacg cacgcaagcc tgcatggttt
     2881 gggcatcgcc tgcatgaaat ccacaagcaa tattggcaga ggaaaccagc gttaatagct
     2941 ctgcgtcgct ggcgcagcct tcgcccagat cggcgttcag gtcaattttc attgtgtagc
     3001 cgccacgcta attgttcgaa ataacgttgt tgatcttgcc gcgctttcag tgcctcttcc
     3061 agtgaacact ggacaaaatg aatcggctga ccgagcggaa tttgcgccag atggtacata
     3121 tcagcctcaa tgatacaggc aatacgcggg taaccaccgg tggtctgtgc gtcgttcatc
     3181 aacacaatcg gctgcccgtt atgcggcacc tgcaccacac ccggtaacaa accgtgagat
     3241 aacagttcgc gatcggtggt gcgttttaaa atttgcccct gtaagcgata gcccatgcgg
     3301 ttactttgcg ggctaagctg ccagggcgaa cgccagaatg catcctgcga ggcgcgatcg
     3361 aactcatgat attccggccc cggcaaggcg cgaatgcggt tgccccacag cagctgttta
     3421 acgccctgcg cttccataaa atcacgcttc gatttgccaa tcgggagtcg gtcaccatcc
     3481 ttcagtaaac ggccttccag cccgccaatc cccacgttga gatcggtgct gcatgacccc
     3541 attaccggcg gaacatcaat accacccgcg accgccagat aactgcgcat gccgtgctgc
     3601 gggcgtttaa gcgttaaacg ctggcctgct ttcatcggca agcgccagcc ggtccagacg
     3661 gcgttatcat ccagccgcgc ttcgcaaccg gcacccgtca gagcaaacca cccatcagtt
     3721 tcgaactcaa ccgttaactg accgagcgtg atctccagcg cgggggcatt ggcgtcatta
     3781 cccaccagca ggttagcaat gcgtaacgca ggcatatcca gtgcgccgca gtggctgata
     3841 cccgactggc gaaaaccgtg acgaccgcca tcctgcacag tggtatacat gcccgcacga
     3901 ataatcttca gcatactccc tccttctgcg gtacaaagcg cacgctgtct cccggacgta
     3961 ataagatggg ttcgtcacgc gccggatcaa acaggctgag tgaggtatga ccaatcaact
     4021 gccagccacc cggcgttgcc agcggataaa caccagtctg cggcccgccg atcccgacag
     4081 aacctgccgg aacgagcaag cgcggttcag cgcgccgtgg cgtgtgtagt tgttccggca
     4141 aactcccgag atacgggaag cccggttgaa aacctaaaaa ccagaccacg tattccacgg
     4201 aggagtgcaa ttcaacaacc tgtttttcgc tcaacccgca atgcgccgcg accaccgcca
     4261 aatccggtcc gcctgcacca ccgtaaacca ccggaatttc aataaagcga gactcaggtt
     4321 ccagggcctc gctctcctcc caccagcgtt gcaaaagctc tatggcatcc agcgccagcg
     4381 actcaggatt acgcaaaatc accgtgatat tgttcatgcc gggaatggct tcaaccacat
     4441 tcggcatatc caccagacgc tgcgccagtc gccagatccg tttctggcta gccagcgtca
     4501 ccggcggttc cagttccagc actaccgccg tttcacctat cagataacaa cgcgctcgtt
     4561 gcactttcga tccctctgat tattcgttat gcaggattag gaatatcaat aaaggtcaca
     4621 tcaagatcgg tattttcatt cagccactcg ctcaatgcgc gaataccacc acgttcagtg
     4681 gcatggtgac ctgcagcata aaaatgcaat ccctgctcgc gggctgaatg aatggtctgt
     4741 tcagaaactt cgccagtaat aaaagcatcc acgccaaaac gcgcggcgct atcgataaaa
     4801 ctttgcccgc cgcccgtgca ccaggcgacg cgctggacca cctcaggtcc ggtatcgcca
     4861 caccataacg gcttacgtcc cagacgcgct tcaatccagg aggctagttc cagtcccggc
     4921 acaggcatgg tcagctctcc ccacggcacc aacggctcaa tttcgcccat gaccgtgatc
     4981 cccagtaacg ccgccagctg tgcgttattg cccagctcag gatgtgcgtc aagcggcaaa
     5041 tgccagccat acaggttgat atcattcgcc agcaacgttt ttaaacggtt acgcttcatt
     5101 ccgcgaatga ccggagactc tcctttccag aagtagccat gatgcacaat gaccgcatcc
     5161 gcgcccagac gcactgcctc atcgagcaaa gcctggctgg cggtgacacc ggtaacaatt
     5221 ttttgcaccg tctctttgcc ttccacctgc aaaccattcg gcgcgtaatc actaatcgcc
     5281 gcgctgttca gtttttcgtt aatcagttgt tccagttcgg tgtttttcat cttcgcgcct
     5341 tccgtattct gatagctaca ccgttaacat agcgttacct cgcctgttcg tcgatagcca
     5401 atcttttgac atattaagaa acaaacccga cgccaaaaga cgcaaattgt ttatatcatc
     5461 acgcttcagc taatgtatat ttatgcaatc agacagcata ttgcataaat aatcagatct
     5521 gtatcaaagt tttaacttta aggaaacaca agcacgccgc gcaactatta cgtgacgttg
     5581 ccgtcaattg tggaattatt atgaataaac acgcatcaca gccgcgcgct atttactatg
     5641 tcgttgcgct gcaaatctgg gaatatttta gcttttacgg catgcgtgcc ctgctgattc
     5701 tctatctcac caatcaacta aaatacaacg atactcacgc ctacgagtta tttagcgcct
     5761 actgttcgct ggtgtatgtc acgccaatcc tcggtggctt tttggcggat aaagttctcg
     5821 gcaatcgcat ggcggtgatg ctgggggcgt tattgatggc gatcggtcat gtggtgttgg
     5881 gtgccagtga gatccatccg tcattcctct atctgtccct ggcgataatt gtttgtggct
     5941 atggtctgtt taaatccaat gtcagttgcc tgctcggcga gctgtatgag ccaaccgatc
     6001 cgcgtcgtga tggcagtttc tcgctgatgt atgcagcggg taacgtgggg tctattatcg
     6061 cacctattgc ctgtggctac gcctaggaag agtacagctg ggcgatgggc tttggcctgg
     6121 cggcggtggg tatgatcgcg ggtctggtca ttttcttatg tggcaatcgt catttcacac
     6181 atacccgcgg cgttaataaa aaagtactgc gtgcgacaaa ctttctcctg cctaactggg
     6241 gatggctgct ggttctgctg gtggcaacgc ctgcgctgat taccgtattg ttctggaaag
     6301 agtggtcggt atacgcctta attgtcgcga ctattattgg cctgggtgta ctggcaaaaa
     6361 tttatcgcaa agcagaaaac cagaaacagc gtaaggagct ggggctgatt gtgacgctca
     6421 ccttcttcag tatgttgttc tgggccttcg cacaacaggg cggcagctcg attagccttt
     6481 atatcgaccg cttcgttaac cgcgatatgt ttggttatac cgttccgact gctatgttcc
     6541 agtcgattaa tgccttcgca gttatgctgt gcggtgtgtt cctggcgtgg gtggttaaag
     6601 aaagcgtcgc aggtaatcgt accgtgcgca tctggggaaa atttgctctt ggtctcggcc
     6661 tgatgagcgc cgggttctgc attctgacct taagcgcccg ctggtccgca atgtatggtc
     6721 actcttctct gccactgatg gtattaggcc tggcggtgat gggctttgcg gaactgttta
     6781 tcgacccggt tgccatgtcg caaattacgc gcattgaaat ccccggtgtg accggcgtat
     6841 taaccggcat ttatatgctg ctttccggcg cgattgcgaa ctatctcgcg ggcgtgattg
     6901 ccgatcagac atcgcaggct tcgtttgatg cttccggggc gatcaactac tccatcaatg
     6961 catatattga agtatttgat caaattacct ggggcgcact ggcgtgtgta ggactggtac
     7021 tgatgatttg gctgtatcag gcgctgaaat tcagaaaccg cgcgctggcg ctggagtcat
     7081 aataggccag caccatacct gataaaacgc tcgctgcgtt ttatcaggct cctttcctcg
     7141 cttcttcgta cgctgccagc gttcgcagcc ttgcctcttt gtgatcaact atcggttgcg
     7201 gataatccag catcacacct gctttctgcg cccacttcca cggctcatgc accgctttcc
     7261 ctggtacatc gcgcagctcc ggtaaccatc gacgaataaa ctcgccctca cggtcaaatt
     7321 tctcgccctg ggttgtcggg ttgaaaatac gaaaatacgg cgctgcatcg gttccggttg
     7381 aagcggccca ctgccagcca ccgttattgg ctgccaaatc accatcaatc agctgcgaca
     7441 tgaaatatcg ctcgccttcg cgccagtcga tcaacaaatc tttcaccaga aaactggctg
     7501 taatcatccg tagccggtta tgcatccagc cagtgctgtt aagctgacgc atggcagcat
     7561 cgacaatcgg gtatcccgtt ttgccttcct gccaggcctg taaatgtgcg ggattgctct
     7621 gccactgcac acgatccgtc caggcaataa acggacaatg tttacacaac gaggggtaat
     7681 acgtcatcag atgacggtag aactcgcgcc agatcagctc actaagccag acactaccgg
     7741 ccccaccgtc cagcgcctgc ggctgttccg ccaacaagcg atgcaaacac tggcgaggcg
     7801 ataacccgcc cgttgccagg ctggcggaca aacgactggt gccttccact gccggaaaat
     7861 ctcgttgttg ctcatattct ccggcaccgt tctggcaaaa ctggcgtaat tgcgcaatcg
     7921 ccgctttttc ttccaccggg aaatgcgcag tatcgaaaga ctgacgagga taattcagcg
     7981 taatggatgg cgagggctct atcgatccgc tactacgaac ttttggcgca gcgacgcact
     8041 ccggcatccc ttcccgcagc cgtttcagcc aggcattctt aaaaggcgta aagactttgt
     8101 acatctcgtg attaccggtc atcaccgcgc caggcggcag gatcacgctg tcatcaaatc
     8161 cttcacacac cacgttacgc agcgctcttt caacttccac atcccgcgcc cgctcattca
     8221 cttcatactg atagttataa aacaggtggg taacgctgtt ttccgcgcac acctgtttaa
     8281 ctatttcgac actggcgaca aagtcatcca cttcacggaa caataaagga ataccttttt
     8341 ccgcaagcgc tatttgtagc ccattcagtt gagcattgat aagttcagcc tgacgcggcg
     8401 acatgttatg cgtcgcccac tggcgtggtg tagcgatata caacgccagc acgcgtgcag
     8461 acgaattgct gcaggcggca gccagtgcga gattatcgtg caaacgtaaa tcctggcgaa
     8521 accagaccag atgggtagtc ataaaactcc tgaatggcgc aagttaatca cacgcggcca
     8581 aagttcgaaa taacgctgcc ctgaaagcca ggcgtcagga taaagcgcca tatagtgttt
     8641 gatacgcatc agcggcgcaa gaagtggttg ctcgccacgg cgataactgt cgataagcgc
     8701 agccagctgc tggcgttctg tggaatcaat atgcgggcga aaataaccct gaacgtgcat
     8761 caaaacattg gtgtgatcgc gcgggttggc ggggtgagaa agcaaaacaa ctacgcgttg
     8821 gcggtactgg ttgtaatagt catccagatt ttgccactgg tgaatatcag ccacgaacgg
     8881 gccaagtttg cggtactccg gctgagaatg cgccagaaag acgagtttat agcggctgtg
     8941 ataatcgaga agcgccccgc gcgttaagcc atgttctttc agtcgatgta actcatgaag
     9001 ggcaaagata cggattaggg tgctgtcatc aaatcgttgt agattcatta ctgctcctga
     9061 acggtataaa ggagtaagtg tagacgatgg taaatgaatt ttatggggca gagtttttgc
     9121 ggggaagtat tttgttggta aaaatattat caacttcatc tgagcgagaa ttattctact
     9181 atttcccgag ctgcattcat tttttgtatt ttttattttt agaaaataac agttctcaaa
     9241 gcttaattac ctcactatta attgtatttc aaactaacaa tcgcactcca ttttctctct
     9301 ttaaaaaaca taaacactgt taaaacaaat atttatttcg ttatcttaaa tgctatttct
     9361 acatcactcg tccttataat caaattattc ttattcatag ctgtttttgc aggtttaaaa
     9421 tagcggcttt caccaaagtc agccatgaaa attagactac aagttctccc agcctttgca
     9481 catgagaaaa accacccatc aactctattt attcctgaac ctttaacacc ataataaatc
     9541 agaatttctg ggtattgttc atccttcacg ttttcatatt tttggtattt aattgaaaac
     9601 caagagtcta tactgccaac atcttcaaaa aatattggag ctgatgtttc cgcatgaaca
     9661 tatgcagaaa atgctaacaa aatggcaata cttagtttgg taaaaatatc gttccacact
     9721 tttcaggata atctattggg gggaagggat cttttcctct aaatctaatt gctcccactc
     9781 cttgtttgtc agaccattcg ccatttgcat cctgacgata ccagtgataa tcccctccca
     9841 tgcctataaa attgtgagga cgaataaaca attgagcttt atggtaacca gatggacaac
     9901 tcccattatc taaatccttg gcaccatcat tttttgacgc ttcaaaaata ctaccgcaag
     9961 tgagtttaga aaactctttt ccagataatt ctccaggttg taatcctccg ccgccaaaaa
    10021 tcctactccc agggtttcca tacctattta gagcatatga ataacaatta tttacggcaa
    10081 gataatacgg atctgaattt aggtctcgat tgaatactgg agcaggttta tcaattaagc
    10141 ccagcg
//
LOCUS       DADLFA010000153        10086 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000153 DADLFA010000000
VERSION     DADLFA010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.155"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            203..355
                     /gene="hokA"
                     /locus_tag="JQH65_003201"
     CDS             203..355
                     /gene="hokA"
                     /locus_tag="JQH65_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HAY6140525.1"
                     /translation="MPQKYGLLSLIVICFTLLFFTWMVRDSLCELHIKQGRYELAAFL
                     ACNLKE"
     gene            complement(543..755)
                     /gene="cspA"
                     /locus_tag="JQH65_003202"
     CDS             complement(543..755)
                     /gene="cspA"
                     /locus_tag="JQH65_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HAY6140526.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(1036..1326)
                     /locus_tag="JQH65_003203"
     CDS             complement(1036..1326)
                     /locus_tag="JQH65_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HAY6140527.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGISVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1740..2450
                     /locus_tag="JQH65_003204"
     CDS             1740..2450
                     /locus_tag="JQH65_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HAY6140528.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQTDDLKPVFDQAFTKVVTTP
                     ADALQPLIPVAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2500..3474)
                     /gene="ghrB"
                     /locus_tag="JQH65_003205"
     CDS             complement(2500..3474)
                     /gene="ghrB"
                     /locus_tag="JQH65_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HAY6140529.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKPSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3578..4237)
                     /locus_tag="JQH65_003206"
     CDS             complement(3578..4237)
                     /locus_tag="JQH65_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HAY6140530.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLDSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4390..6713
                     /locus_tag="JQH65_003207"
                     /pseudo
     CDS             4390..6713
                     /locus_tag="JQH65_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin guanine dinucleotide-containing
                     S/N-oxide reductase"
     gene            complement(6682..7122)
                     /locus_tag="JQH65_003208"
     CDS             complement(6682..7122)
                     /locus_tag="JQH65_003208"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY6140531.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDSKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAINFYQAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7119..7682)
                     /gene="tag"
                     /locus_tag="JQH65_003209"
     CDS             complement(7119..7682)
                     /gene="tag"
                     /locus_tag="JQH65_003209"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAY6140532.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7840..8538
                     /locus_tag="JQH65_003210"
     CDS             7840..8538
                     /locus_tag="JQH65_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY6140533.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8766..9968
                     /locus_tag="JQH65_003211"
     CDS             8766..9968
                     /locus_tag="JQH65_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY6140534.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHI"
BASE COUNT         2424 a         2429 c         2582 g         2651 t
ORIGIN      
        1 ttttaggatt atatcaagga gaagaaacaa acttattaag ctagaatagc tacgggtgct
       61 tgagactgtt tgtctcaggt attcaccgaa aggcagacag agaaaagccc cacctgacta
      121 taaatcaaag tgaggctacc ctatgcctga acaccataag gttagcctct tactcgttgg
      181 aaatcaacac agggggctgg gaatgccgca aaaatacgga ttactttcat taatagtgat
      241 ttgtttcacg cttttatttt tcacctggat ggtaagagat tcactgtgtg aattgcatat
      301 taaacagggg cgttatgagc tggcggcatt tttagcctgc aatttaaaag agtaagagtc
      361 ttcggcggga aattattccc gcctttctta cggcgttgcg cattctcatt gcacccacat
      421 ttattcttca caaaaataat aatagatttt actacgcgat cgattattta tttcctgaaa
      481 aaaaataaaa aaaatccccg ccaaatggca gggatcttag attctgtgct tttaagcaga
      541 gattacaggc tggttacgtt accagctgcc gggcctttag cgccgctttc gatggtgaag
      601 gacactttct gaccttcgtc cagagatttg taaccatcgt tctggatagc agagaagtgt
      661 acgaacacat ctttagagcc atcgtcagga gtgatgaagc cgaagccttt gtcagcgttg
      721 aaccatttta cgataccagt cattttaccg gacatagtgt attaccttta ataattaagt
      781 gtgcctttcg gcgatatggc gtgctttaca gattttgaag cgttaaagga atgtgcacta
      841 cgaggggtat caacgataac tcttgaaggg acttgcctta ctacactgct ttaatggtct
      901 gtacgtcaaa ccgttgatgt gcattaagcc acgcattggc gggtgatgca acaattattt
      961 ttcatattta tgattaatcg gttggaaaac ggtcctgtca tcaggaccgt aaacagcaat
     1021 aaagtggata aaagtctatt ccatcaactg cttacttaat gccgggttgg cttgaatcaa
     1081 acgcatcaat tttagttcgg cacttgaagg cttcacgcgt ctggattccc attcctttac
     1141 catggcgact gatatgccca aaacccgggc gaaatcatcg atttttaacc ctgtcccttt
     1201 tcgtaactgc tcaatttcgg tacaggacgt tgttctgtgc gtcagggtaa ttttctgcgt
     1261 ttcatcttta aaaacaatct gttccaggct gctcaacagc tcatgcattg gatctttata
     1321 ttccattgaa aactcctcaa atcacactgc gggatcgtga attgcatcga agctcattaa
     1381 gaatagtcgg gaaaagcaga cacagcgggg tgaagtcagg gcatgctctg gctatgattc
     1441 gattcagcca gtcatttagc gtgacttata gccacctaac gatctgataa atgccgatat
     1501 atctcttacc actcctgttt tgtaaagatt tcgaaaatct aaagttctgc gaaggcgtaa
     1561 atctcgggct acaaaaaagc aacggcaata tcatttttta acaatgaaac aacaaattaa
     1621 ccttgtttca ttttctccat gattttacca ggaagattac gtagaaaggg tatcctgcaa
     1681 gcctgtcctg gactatcctt gtcacgtcag acacgctttt gggtgaaagg agtaagaaaa
     1741 tggcgacagg aaagtcctgc tctcgctggt ttgcgcctct tgcggcgtta ttaatggtag
     1801 ttagcctgag tgggtgtttt gataaagaag gcgatcagcg taaagcgttt atcgacttcc
     1861 tgcagaatac agtgatgcgt agcggtgaac gtctaccaac cctgactgcc gatcagaaaa
     1921 aacagtttgg tccttttgtc tctgattacg cgattctgta tggttattct cagcaggtga
     1981 atcaggcgat ggattccggt ctgcgcccgg tcgtagacag cgttaacgca attcgcgtac
     2041 cgcaggatta cgttacgcaa agcggtccgc tgcgtgaaat gaacggttcg ctgggtgtgc
     2101 tggcgcaaca actgcaaaat gcgaagttgc aggccgatgc tgcgcactct gcgctaaaac
     2161 agaccgatga tttgaaaccg gtctttgatc aggcattcac caaagtggta acgacgccag
     2221 ctgatgcatt gcaaccgttg attccggttg cacaaacttt cacgcaacaa ctggtgatgg
     2281 ttggagacta cattgctcaa caaggtactc aggtgagctt tgtagcaaat ggtatccagt
     2341 tcccgacttc gcagcaggca agtgaatata acaaactgat tgcgccgtta ccggcacaac
     2401 atcaggcgtt taatcaggcc tggactacag cagttactgc aacccaataa agagtaaaag
     2461 cccgagcgat tatcgggctt tacttcacgc agtcgcggct tagtccgcga cgtgcggatt
     2521 cacacagttc ttctcaacct ttccttgtaa cgcatcaatc aaattatcca cggcacaggc
     2581 ggccatgcca taacgcgtct catgggtggc agatccaata tgcggtactg cgacgacgtt
     2641 ggccattgag agcaacggcg aatctacgga cagtggctct tgttcgaaga catccagccc
     2701 ggcggcgtga atttcgcctt tctgcaatgc tgcgatcagt gcattttcgt caaccaccgg
     2761 gccacgtccg gcattaatga aaatggcgga gggtttcatt ttggcgaatt gttctgcgcc
     2821 aaacagatga tgcgtctcat cagttaacgg caggatcagg caaacgaaat ctgactcttg
     2881 taacagagta tccaaatcgc agtagcgggc gttgaagcgt tcttctgctt ctttatggtg
     2941 gcggcgcgcg ttatagagga tgggcatgtt gaagccaaag tgcgcacgtt gtgccagcgc
     3001 catgccgatc cgtcccatcc cgacaatgcc cagtgtttta tggtgaacgt cagtgccgta
     3061 ccagtccggg cctatgctcg cggtccattc gcctgctttt acccgttctg ctacctccac
     3121 aacccgacga gcggtagaca acaccagcgc catcagcgta tcggcgacgg tttctgttaa
     3181 tacggttggc gtgtgcatca gcagaatttt tcgggcggta agcgcatcga catcaaaatt
     3241 gtcatagccg acggagatcg ttgatgtggc acgcagtttc ggcatttttt ccagcaatgc
     3301 ggcatctaca ttctcgtttg aacccagtaa accttcagct tcggcaaaaa ttgctgcatt
     3361 ttgttcgacg gtttgtgggc tgaggtttgc cacctggtga acggtgaaat gctcttgcag
     3421 gcgttgcagt aaatcatcag gtaaggcttt gtagaggata acggacggct tcatgcttct
     3481 ctccattcac tgataaggcg ctcaccttag cctgaaatat gatgaaggaa aactgaagaa
     3541 tgccaggtga tttctgcatc acctggcatg aaagggatta cagcgggctt aaggtaattt
     3601 ctacacggcg gttttgcgcc ttaccttctg cggtgctgtt gctggcgatt gggttagccg
     3661 ggccaaggcc ctgagtacgg atgcggctgg cgtccacgcc ctgggtgatc aacgcgctgg
     3721 caacggaatc cgcacgctgc tgggagagac gcatgttcag gtcgtgaccc cccgtgctgt
     3781 cggtataacc aatcacgtta accgccgttt tcggatactc tttcagcacc attgccacgc
     3841 cggtcagggt gttagcgcct gccggtttca gggtcgcgct gctgctgtcg aaggtcacat
     3901 tgttcggcat attgaggata atgttatccc cgctgcgggt tacgctaaca ccagtgccgc
     3961 gcattttgtc acgcagcttc gcttcctgca catccatgta ataacccacg ccgccgccca
     4021 gagctgcgcc tgctgctgcg ccaatcagcg cgcctttacc gcgatctttc ttcgaagaag
     4081 agagcgcacc aatacccgcg cccacgagag agtccagacc tgcgccgata gcagatttgc
     4141 ctgcttcgcg ttcgccggtg taagggttag ttgtgcagcc agataccgcc agagcgccac
     4201 tcactacggc ggcaataaga taaacacgtt tcttcattgt taatccttaa taaccttttt
     4261 attctttgcc acgggttccg tggcgggaga ttatgccgcg tgaacatgaa gattattcct
     4321 gggaatactc ggaaatttgt aagtaatatt taactgctca atacatctaa cctttcagga
     4381 gtcttcggtt tggccaactc atcctcacga tattccgttc tgactgccgc ccactggggg
     4441 cccatgctgg ttgaaaccga cggcgaaacc gtgtttagct cgcgtggcgc gttagccaca
     4501 ggaatggaaa actccttgca gagcgcggtt cgcgactagg ttcacagcaa tacgcgggta
     4561 cgatttccaa tggtgcgcaa aggctttctc gcgtcaccgg aaaacccgca aggcattcgt
     4621 gggcaggatg aatttgttca cgtgagttgg gatgaggcgc tggatcttat tcaccaacaa
     4681 cataaacgca ttcgtgaggc ttatggtccg gcatcgattt ttgctggttc ctacggctgg
     4741 cgttcaaacg gcgtgcctcg acattattac aacgctatat ggcgctggca ggcggttata
     4801 ccgggcatct gggggattat tcgaccggtg cggcacaggc gatcatgccg tatgtcgtgg
     4861 gtggcagcga agtttatcaa cagcaaacca gttggccgct ggtgctggaa catagcgatg
     4921 tcgtggtgtt gtggagtgct aacccactca atacgctgaa aattgcgtgg aatgcatctg
     4981 atgagcaggg gctttcttac ttttccgcac tgcgtgacag cgggaaaaag ctgatctgta
     5041 ttgatccaat gcgatcggaa accgtcgatt tctttggcga taaaatggag tgggtggcac
     5101 cgcacatggg caccgatgtt gcgctgatgc tggggatcgc ccatacgctg gtggaaaatg
     5161 gttggcacga cgaagcgttt ctggcgcgtt gcaccacagg ttatgccgtc ttcgcctctt
     5221 atttgctggg cgagagtgac ggaatagcca aaaccgccga atgggcggca gggatttgtg
     5281 gggttcacgc agcgaaaatt cgcgagttgg cggctctttt ccaccaaaac acgaccatgc
     5341 tgatggctgg ctggggaatg cagcgccaac agcttggtga gcaaaaacac tggatgatcg
     5401 tcacgctggc ggcaatgttg gggcaaatcg gcacacccgg cggcggtttt ggtctttctt
     5461 atcattttgc caatggtggt aatcccacgc ggtgttctgc ggtgctctct tccatgcagg
     5521 gtagcttgcc gggtggcagc gatgcggtag ataaaatccc tgttgcccgt attgttgaag
     5581 cactggaaaa ccctggctgc gcataccaac acaatggcat gaatcgcctc ttcccggata
     5641 ttcgctttat ctggtgggcg ggtggcgcca acttcaccca tcatcaggac actaatcgcc
     5701 tgatacgagc ttggcaaaaa ccggagctgg tagttatctc cgaatgcttc tggacggcgg
     5761 cggcaaaaca cgcggatatc gttctgcctg cgactacctc ttttgagcgt aatgatctca
     5821 ccatgaccgg cgattacagt aatcagcatc tggtgccgat gaagcaagtg gtgccgccac
     5881 gctatgaagc gcgtaatgat tttgatgttt ttgccgagtt aagtgaacgc tgggagaagg
     5941 gcggttatgc acgttttacg gaaggaaaaa gtgagttgca atggctggaa acgttttata
     6001 acgttgcccg acagcgcggg gcaagccagc aggttgaatt gccgccattt gctgagttct
     6061 ggcaagccaa tcagttaatt gagatgccgg aaaacccgga cagcgagcgg tttattcgct
     6121 tcgctgattt ttgccgcgat ccgcaggcgc atccattaaa aaccgccagc ggtaagattg
     6181 aaatcttctc gcagcgtatt gccgattacg cttatccgga ttgcccgggg catccaatgt
     6241 ggctggagcc ggacgaatgg cagggcaatg ccgaaccgga acagttacag gtactttctg
     6301 ctcatccggc gcatcgtctg cacagccagc tgaattacag ttctctgcgc gaattgtatg
     6361 cggtggcaaa tcgtgagcct gtcaccattc atcctgacga tgccctggcg cgcggcattc
     6421 aagatggcga tactgttcgg ctgtggaacg cacgcgggca aattcttgcc ggagtggtca
     6481 ttagcgaggg aattaaacct ggcgtgattt gtattcacga aggggcatgg ccggatttgg
     6541 atttaaccgc tgacggtatt tgtaaaaacg gcgcagtcaa cgtgctgacc aaagatctcc
     6601 ccagctcgcg gctggggaat ggctgtgcgg gtaatacggc gctggcatgg ctggaaaaat
     6661 acaacggtcc ggaactgaca cttacagcgt ttgaaccacc ggccagctca taatccaggt
     6721 gggtagttgg gtttcatcct gccatgcgca atcgacaatg tgaaaaccct gcgcctggta
     6781 aaaatttatc gccggttgat ttttttgata aacctccagc atcaggtggg gatggcgctg
     6841 ctgcacatat tgcatcagcg ccttaccaat accgcgcctg acggccttcg gtgcgacaaa
     6901 catcgctgcc agaaatcggc cttccataat gctgacaaaa ccgagaagct tactgtcttc
     6961 ttcccagacc cagttttgcg cattggcaag atacgcatcc cgcaccagcg gaatgcaatc
     7021 acgccagtaa ttcgctttta taaagggatg tccccaggtt gtactttcca gccacagttc
     7081 gaggatcgcg gggagttctg aacgttgcgc ttcccgaatc atggtttatt tcccggatag
     7141 caacagcagc caaccacatg atcattcacc agcccacatg cctgcataaa ggagtaacag
     7201 attgtggtac cgacaaactt aaaaccacgt tttttcagtg ctttagatag ggcgtcggag
     7261 gcgggcgtag atgtggggat ttcgctcaac gttgtggctt gtgtcacctg tggctgatga
     7321 tttacaaacg accagacaaa gtcggcaaac ggttcgccgt tctgttccat ttgcaggtac
     7381 gcccgcgcat taccaataat tgcctgaatt ttcccgcgat ggcggataat cccggcgtcc
     7441 tgtaccagtc tttcgacatc ctcttcctgc attgctgcga ccttcaccgg atcgaactga
     7501 tgaaagcagg cgcgatagtt ttcgcgtttt ttgaggacgg tgatccacga taacccagcc
     7561 tgctgccctt caaggcagat catttcgaac agttttttac tgtcagtttc aggcacgccc
     7621 cactcattat catggtaggc aatataaagc gggtcctgac tcacccagcc gcaacgttcc
     7681 atactttccc tcgctatgta cccgatcgta aaaatttcag ccgcttttct tgacgcatcg
     7741 gtgaaaaagt caatatttaa tacagggcta ctatgcccga tatccttcat acaatgacaa
     7801 taatattgcc gaattcggct cttccctgga gtcgtgttaa tgatcataaa aaaaagcggt
     7861 ggtcgctggc agctaagcct gctggcgagc gtggtaatca gcgccttttt tctcaacaca
     7921 gcttacgcct ggcaacaaga atatatcgtt gatacgcaac ccggacattc cacagagcgt
     7981 tacacctggg atagtgatca tcaacctgat tacaacgata ttttgtcgca acgtattcaa
     8041 agtagccaaa gggcgctggg actggaagtc aatctggcgg aagaaactcc tgtggatgtg
     8101 accagcagta tgagtatggg ctggaatttt cctttatatg aacaggttac aaccggcccg
     8161 gtcgcggcat tacattacga tggcacaacc acctcgatgt ataacgagtt tggcgacagt
     8221 actaccacgc tgaccgatcc gttatggcat gccagcgtga gtagcttagg ctggcgtgtt
     8281 gactcccggc ttggcgatct ccgaccctgg gcgcaaatca gctataacca gcaatttggc
     8341 gagaatatct ggaaggcgca atcaggcctg agccggatga cggcgacaaa ccagaacggc
     8401 aactggctgg atgtcaccgt aggcgctgat atgttgctca atcaaaatat tgccgcctat
     8461 gccgcgctaa ctcaggcaga aaataccact aataatagcg actatctgta tacgatgggg
     8521 gttagcgcca gattttaacg taacagtcac aattgaaacc attgaataac aatagttgtg
     8581 gtgatagtgg gtgctaactt acaaataata aatttggtga ataattgtcg cgtcattcat
     8641 tcctgaacta aggcatttca ttccgttctg atggcatttc atgccgtttt tccccaggca
     8701 taaaatgcac ttcgttatgg ttgtcggcag agatttttcc tttttattac tgcaggaata
     8761 ctgccatgac accttcaaat tatcagcgta cccgctggct gacactcatc ggtactatca
     8821 ttacccagtt tgcgctgggg tcggtttata cctggagcct gtttaatggc gcgctttctg
     8881 ccaagctgga tgcgccggta agccaggtcg ctttctcttt cggcttgtta agtctggggc
     8941 tggcaatttc atcttctgtt gcggggaaat tacaggaacg ttttggcgtt aaacgcgtca
     9001 ctatggcttc cggcattttg ctaggattag gcttcttcct gacagcgcat tctgacaacc
     9061 tgatgatgct gtggttaagc gccggtgtgc tggtcggtct ggcggatggc gcgggttacc
     9121 tgctgacgct ctctaactgc gtgaagtggt tcccggagcg taaaggtctg atctccgcgt
     9181 tcgctatcgg ttcttatggt cttggtagcc tgggtttcaa atttatcgac acgcagctgc
     9241 tggaaacggt gggtctggaa aaaacatttg tgatttgggg agcgattgcg ctggtgatga
     9301 ttgttttcgg cgcaacgtta atgaaagacg caccaaaaca ggaagtgaaa accagcaatg
     9361 gtgtggtgga gaaagattac acgctggcag agtcgatgcg taaaccgcag tactggatgt
     9421 tagcggtaat gttcctgact gcctgcatga gcggcctgta cgtgattggg gtagcgaaag
     9481 atatcgccca aagtctggca caccttgatg tggtttccgc agccaatgca gtaacggtta
     9541 tttccatcgc taacctttca ggtcgtctgg tgctgggcat tctgtctgac aaaattgccc
     9601 gtatccgtgt tatcaccatt ggtcaggtga tttcgctggt gggtatggcg gccctgctgt
     9661 ttgcaccatt aaatgcagtg acgttctttg cagcgattgc ctgcgtggca tttaactttg
     9721 gcggcactat taccgtcttt ccatcactgg tcagtgagtt cttcggcctc aataacctgg
     9781 cgaaaaacta cggtgtgatt tacctcggtt tcggtatcgg tagcattttt ggttcgatta
     9841 tcgcctcact gtttggcggt ttctatgtga ccttctacgt gattttcgcc ctgctgattc
     9901 tgtcattggc gctttctacg acgattcgtc agccagagca gaaaatgttg cgtgaggcgc
     9961 atatttagaa atatgttgcg actgaaataa atacgaggag ggagacttca aagtctccct
    10021 tttttatgga cgcatattaa aagaacaata atattcatac cacaaatata ttttatttat
    10081 atatta
//
LOCUS       DADLFA010000154         9985 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000154 DADLFA010000000
VERSION     DADLFA010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9985
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.156"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            289..486
                     /locus_tag="JQH65_003212"
     CDS             289..486
                     /locus_tag="JQH65_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6140535.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(436..1629)
                     /locus_tag="JQH65_003213"
     CDS             complement(436..1629)
                     /locus_tag="JQH65_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY6140536.1"
                     /translation="MSPGIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFVTDTTAISQQVAWLLGLSAVAALIAQFGV
                     VRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            1765..3489
                     /gene="iucA"
                     /locus_tag="JQH65_003214"
     CDS             1765..3489
                     /gene="iucA"
                     /locus_tag="JQH65_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HAY6140537.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3490..4437
                     /gene="iucB"
                     /locus_tag="JQH65_003215"
     CDS             3490..4437
                     /gene="iucB"
                     /locus_tag="JQH65_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HAY6140538.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4437..6179
                     /gene="iucC"
                     /locus_tag="JQH65_003216"
     CDS             4437..6179
                     /gene="iucC"
                     /locus_tag="JQH65_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HAY6140539.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFVEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDEGPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDATSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6176..7513
                     /gene="iucD"
                     /locus_tag="JQH65_003217"
     CDS             6176..7513
                     /gene="iucD"
                     /locus_tag="JQH65_003217"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HAY6140540.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVITLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7519..9714
                     /gene="iutA"
                     /locus_tag="JQH65_003218"
     CDS             7519..9714
                     /gene="iutA"
                     /locus_tag="JQH65_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HAY6140541.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLSLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
BASE COUNT         2400 a         2569 c         2655 g         2361 t
ORIGIN      
        1 tgtctggaga ttcagggggc cagtctactt cataaaaaac atgtcagtga aaaagaaacc
       61 tggcgttggg ttgaaggata taataaagca acagaactgg ctcgccaatg ccctgatacc
      121 catgtattgt caatttctga tcgtgaaggc gatttttatg acctgtttga acgcgcagag
      181 cagactcccg gaataaaagc tgactggctt gtcagaatga aatttaataa cagagcaacg
      241 ctgaatatta atggtaaacg ggatcatagg ttgttgcacg aaagaatcat ggagattaca
      301 ccgcagcaac tggttgaatt cactatacct gatgggcggg ggcagcaagc cagaacactg
      361 cctgacatgc tttcatcaga cgcaggtcct ctctcatatc ctctgattta ctccagtaag
      421 gtgatgatca tatctttatt tgatagtacg gtaatgccag ataaatacag acacaatggt
      481 aaataatata gttgcaaata atgctgccat aatcagcgcg attgcgacac ctgttttcga
      541 taccagcggc accagcaaag cgcacagtcc atagccaagt gtgtgactgg tagcgagcca
      601 gcccgcgcct gcgccatcag ccagcttatc attcagtaga agttgataag cgggtgttgc
      661 cagagctgct ccaaatgaca gcactgcaca gcccgggtaa aacaaccata gctgttcgga
      721 gagcatgata gccagaccac cactcatcaa cactccggca ctcaggagca gggccaccgg
      781 agtcaggcgc tgcggacgga ctaccccgaa ctgcgcgata agcgcagcta ctgcggacag
      841 acccaacagc caggccacct gttggctaat ggcggtggta tccgtgacga actggcgagt
      901 aagggcaggc gaaagtccaa gctgcatcat gctcaccgcc gcagccagta atattgcgca
      961 aagcagataa ggcagacaat cccgcttcag actgacgctc ttgcactccg gtgtgggctg
     1021 tggtggtgtg ccgggcaacc gcagcagcat cagcagcgcc agcactggtg ctgccatcag
     1081 tagccccagt ggcgccagag gatgaatggc cagcattgct gccgcgcaca gcggcccgaa
     1141 tagcctgccg caactcaggc cggagctgat ggttgccaga gcggccatgc gattcccttc
     1201 tccagctctc tgcaatgccc agacctgaca ggctggcacc attgccgaca cggtcagccc
     1261 gtaggcgatc cgggcgatga ttaatattcc cagtcccacc gctgttgtta tggcgctggt
     1321 agccatcagc acgcttccca gccctagcag ggtaaagctt atgccatatc ctcccagagc
     1381 ccacaacacc acaggtttac tgccagtacg ggaaatttgc tttccccacc acggggacga
     1441 tggcagaaac agcattgagc caagcatcag cagggcagcc catacagaca gactcagatt
     1501 tgtctgtatg accagaacag ggagcacaac cagcagaccg ttctgtccga taccgagaag
     1561 cccggcactg aacgcaagtg gccagcagga cagtgttttt tggggcgtat cttcgatccc
     1621 tggtgacatt ataatgcttc aactccatgt attaattgtg tttatttgta aaattaattt
     1681 atctgacaat aacatttatt attgataatg agaatcatta tcgacataat tgttattatt
     1741 ttactgtgtg ggagctgttt gactatgacc ctgccctctg aaaaaccagc cacagatgtg
     1801 gctgcgcagt gcttcctgaa tgcactgatt cgtgaaacca cagactggaa actgacagaa
     1861 tacccgccag acgaattgct catcccgctg gatgagcaga aatcgctcca tttcagagtg
     1921 gcttatttct ccccaaccca gcatcaccgc tttgcatttc ctgcacgtct ggtcacggca
     1981 tcaggcagtt atcctgtcga ctttaccact ctctcccggc ttattattga taagctacgc
     2041 catcaactgt ttctgcccgt tcccctctgc gaaactttcc accagcgcgt gctggaaagc
     2101 catgtccata cgcaacaggc aattgatgcc cgtcatgact gggccgctct gcgtgaaaaa
     2161 gcgttgaatt ttggcgaggc tgagcaggcg ctgctgacag gacacgcttt ccaccctgcg
     2221 cctaagtctc atgaaccgtt taaccggcgg gaggctgaac gctacctgcc tgatatggca
     2281 ccccacttcc cactgcggtg gttttcggtg gataaaacgc aaatcgccgg tgaaagtttg
     2341 catctcaacc ttcaacagcg gctgacgcga tttgccgcag agaatgcgcc tcagttactc
     2401 aacgaattaa gtgacaacca atggctgttc ccgttgcacc cgtggcaggg ggaatatctt
     2461 ttgcagcagg ggtggtgcca ggcacttgtt gctaaagggc tgattaaaga cttaggtgag
     2521 gccggcacgt cgtggctgcc gaccacctct tcccgttccc tctactgtgc caccagccgc
     2581 gatatgatca agttctccct gagcgtacgg ctgaccaact ccatccgtac tctgtccgtg
     2641 aaagaagtga agcgtgggat gcgcctggca cgcctggctc aaaccgacgg ctggcagatg
     2701 ctacaggtcc gcttcccgac tttccgggta atgcaggagg atggctgggc cgggctgctc
     2761 gatcttaacg gcaacatcat gcaggaaagt ctgtttgccc tgcgtgaaaa tctgctggtg
     2821 gatcagccga aaagccagac taacgtactg gtctccctga ctcaggccgc acctgatggc
     2881 ggtgattcgc tgctggtttc ggcggtaaaa cgcctgagcg atcgcctcgg tatcactgtg
     2941 caacaggccg cccatgcatg ggtcgatgca tactgtcagc aagtgctaaa gccgctgttt
     3001 acggctgaag cggattacgg cctggtgctg ctggcgcatc agcaaaatat tcttgtccag
     3061 atgcttgggg atctgccggt cggatttatt taccgtgact gtcagggcag cgcttttatg
     3121 cctcacgcga cagactggct cgattctatt ggcgaggcgc aggcggaaaa tattttcacc
     3181 catgagcagt tgctgcgcta tttcccttat tacctgctgg ttaactccac ttttgctgtg
     3241 accgctgcgc tgggggctgc cgggctggac agcgaatcga atctgatggc tcgtgtacga
     3301 gcatcgctgg ctgaagtgcg tgatcaggtg actcataaaa cctgtctcaa ctacgtgctg
     3361 gaaagtccgt actggaacgt aaaaggtaac tttttctgtt atctgaacga tcataacgag
     3421 aacaccattg ttgacccttc ggtgatctat ttcgatttcg ctaacccgct gcaggctcag
     3481 gaggtctgaa tgtctgaggc aaacattatt cacagcagat atggactgcg ctgtgaaaaa
     3541 ctcgacaagc ccctgaatct tggctgggga ctggacaata gcgcggtgtt gcactgtccc
     3601 ggggagctgc cgacagggtg gctgtgcgat gcgctggatc agatatttat cgccgcacca
     3661 caactgtcag cagtggctct cccctgggcc gaatggcgtg aggagccaca ggcgctgacg
     3721 ctttttgggc aggtaaaaag cgacattatc catcgtactg ccttctggca gttaccgtta
     3781 tggttgagtt caccggcaaa ccgggcctcc ggtgaaatgg tttttgatgc agagcgtgag
     3841 atttatttcc cgcagcgccc accccgtcca cagggtgagg tttaccgccg ttacgatcca
     3901 cgtattcgca ggatgctgag tttccgcatt gccgatcccg tttctgatgc agaacgtttc
     3961 acccgctgga tgaatgatcc gcgcgttgag tatttctggg agcaaagtgg ctcactggag
     4021 gtacagaccg cctatctgga gcgccagctc accggtaaac atgcattccc gctgattggg
     4081 tgcttcgacg atcggccatt cagctatttc gaaatctact gggcggcgga agaccgcatt
     4141 ggtcgtcact attcgtggca gccctttgac cgtggcctgc atctgctggt tggtgaacag
     4201 caatggcgcg gggcccacta tgtgcaaagc tggctgcgcg ggctgacaca ttacctgctg
     4261 ctggatgagc cccgtacgca gcgcacagta ctggagccac gcaccgataa ccagcgcctg
     4321 ttccgccatc ttgagcctgc gggataccgg acaattaaag agttcgactt cccacataag
     4381 cgctcgcgca tggtgatggc ggatcgtcat cacttcttca cggaggtcgg tctgtaatga
     4441 atcacaagga ctgggatttt gttaaccgtc ggctggtggc aaaaatgttg tctgagatgg
     4501 agtatgagca ggttttccac gcggaatctc agggcgatga ccactactgc attaacctgc
     4561 cgggagcaca atggcgcttc atcgctgaac gtggtatctg gggctggctg tggattgatg
     4621 cccaaacgtt gcgctgcacg gacgaaccag tactggcgca gacgctgctg atgcagctaa
     4681 agccggtgct gtcaatgagt gatgccactg ttgcagagca tatgcaggat ttgtatgcca
     4741 cgctactggg cgacctgcaa ctgctgaagg cccgtcgcgg actgagtgcc agtgacctga
     4801 ttaatcttga tgccgatcgt ctgcaatgcc tgctgagcgg tcatcctaaa ttcgttttta
     4861 ataaaggtcg ccgtggctgg ggcaaagagg cgctggaacg atatgcgcca gagtatacca
     4921 acaccttcag actgcactgg ctggcggtaa aacgtgaaca tatgatctgg cgctgcgata
     4981 acgatctgga tattcagcag ttgttgactg ccgcaatgga tccgcaggag tttacacgct
     5041 tcagtcaggt ctggcaggaa aacgggctgg atcacaactg gctaccgctg ccggtacatc
     5101 cgtggcagtg gcagcaaaaa atcgccacag acttcatcgc tgattttgtc gaaggcagga
     5161 tggtgtctct cggcgagttt ggcgaccagt ggctcgccca gcagtcgctg cgtaccctga
     5221 ccaacgccag ccgccgggga gggctggata tcaagctgcc gctgaccatc tacaacacct
     5281 catgctaccg ggggatccct ggcagatata tcgctgccgg accactggct tcacgctggc
     5341 tgcaacaggt ttttgcgacc gacgccaccc tggtgcaaag cggcgcagtg atccttggtg
     5401 aaccggcggc aggctatgtg tcccatgaag gctatgccgc gcttgcccgg gctccctatc
     5461 gctaccagga aatgcttggt gttatctggc gggagaatcc gtgccgctgg ctgaaaccgg
     5521 atgaaggtcc tgttctgatg gcaacactga tggagtgcga cgaaaacaat cagccgctgg
     5581 caggcgcata tatcgaccgc tccggactgg acgctgaaac ctggcttacg caattgttcc
     5641 gggtggttgt ggttccgctg tatcacctgc tttgccgcta cggtgtcgcg cttattgcac
     5701 acggacaaaa tataacgctc gccatgaaag agggggtacc acagcgtgtt ctgctgaaag
     5761 acttccaggg cgatatgcgg ctggtgaaag aagcgtttcc cgaaatggat tctttgcctc
     5821 aggaggtgcg tgatgccaca tcccgtctga gtgcggacta cttaatccat gatttgcaga
     5881 cgggtcactt cgtgacagta ctgcgtttta tttcgccact gatggttcgt cttggcgtac
     5941 ctgaaaggcg attttatcaa ctgctggcag cagtgttgag tgattacatg aacaaacatc
     6001 cacaaatggc agagcgtttt gcgcttttct cactcttcag gccacaaatc attcgcgtgg
     6061 tgctgaaccc ggtaaaactg acctggccgg acctggatgg cggcagtcgc atgctgccga
     6121 attaccttga gaatctgcaa aatccgctgt ggctggtaac tcaggaatat gaatcatgaa
     6181 aaaaagtgtc gattttattg gtgtcgggac agggccattt aatctcagca ttgctgcatt
     6241 gtcacatcag atcgaagaac tgaactgtct cttctttgac gaacatcctc atttttcctg
     6301 gcatccgggt atgctggtac cggattgtca tatgcagacc gtctttctga aagatctggt
     6361 cagtgcagtt gcacctacaa atccctacag ctttgttaac tatctggtga agcacaaaaa
     6421 gttctatcgc ttccttacaa gcaggctacg tacagtatcc cgtgaagagt tttctgacta
     6481 tctccgctgg gctgctgaag atatgaataa cctgtatttc agtcataccg ttgaaaatat
     6541 tgatttcgac aaaaaaagtc gattgtttct ggttcagacc agtcggggag aatattttgc
     6601 ccgcaatatc tgccttggta caggaaaaca accttattta ccaccctgtg tgaagcatgt
     6661 gacacaatcc tgtttccatg ccagtgaaat gaatcttcgt cggccggacc tgagtggaaa
     6721 acggataacc gtggttggtg gaggacagag tggtgcagac ctgttcctta atgcattacg
     6781 cggggaatgg ggagaagcgg cggaaataaa ctgggtctca cggcgtaata attttaacgc
     6841 actggatgag gctgcttttg ctgatgagta ttttacacct gaatatattt caggcttctc
     6901 cggactgaag gaagatattc gccatcagtt actggatgag cagaaaatga catcggatgg
     6961 catcactgcc gattctttac tgaccattta tcgtgagttg taccaccgtt ttgaagttct
     7021 gagaaaacca agaaatatcc gtctgctacc cagtcgctcg gtaataactc tggaaagtag
     7081 tggtccgggc tggaggttat tgatggaaca tcatctggat cagggcagag agagcctgga
     7141 aagtgatgtg gtgattttcg ccacaggtta ccgttctgca ttgccacaaa tacttccctc
     7201 actgatgccc ttgatcacca tgcacgataa gaacaccttt aaagtgcgtg atgacttcac
     7261 tctggaatgg agtggcccga aagaaaataa catctttgcg gtcaacgcca gcatgcaaac
     7321 tcatggcatc gccgaacccc agctcagcct gatggcctgg cgatctgcac gtattcttaa
     7381 tcgcgtactg gggcgtgatt tattcgatct cagtatgccg cccgcactga ttcagtggcg
     7441 cagcggcagc cggaaaaaac cgcagccgga ggctgcttct ttaactcact atacagcaaa
     7501 tattcaggaa taacgatgat gcgcaaaaag tatatgcccc gggctcttgg tccgctgctt
     7561 cttgtcgtgc tgtcaccagc tgtcgcccag caaaatgatg ataatgagat catagtgtct
     7621 gccagccgca gcaatcgaac tgtagcggag atggcgcaaa ccacttgggt tatcgaaaat
     7681 gccgaactgg agcagcagat tcagggcggt aaagagctga aagacgcact ggctcagtta
     7741 atccccggcc ttgatgtcag cagccagagt cgaaccaact acggtatgaa catgcgtggc
     7801 cgcccgctgg ttgtcctgat tgacggtgtg cgcctcaact cttcacgttc cgacagccga
     7861 caactggact ctgtcgatcc ttttaatatc gaccatattg aagtgatctc cggcgcgacg
     7921 gccctgtacg gtggcgggag taccggaggg ttgatcaaca tcgtgaccaa aaaaggtcag
     7981 ccggaaacca tgatggagtt tgaggctggc acaaaaagtg gctttaacag cagtaaagat
     8041 cacgatgagc gcattgccgg tgctgtctcc ggcggaaatg accatatctc cggacgtctt
     8101 tccgtggcat atcagaaatt tggcggctgg tttgacggta acggcgatgc caccctgctt
     8161 gataacaccc agaccggcct gcagcactcc aatcggctgg acatcatggg aaccggtacg
     8221 ctgaacatcg atgaatcccg gcagcttcaa ctgataacgc agtactataa aagtcaggga
     8281 gacgacaatt acgggcttaa tctcgggaaa ggcttttccg ccatcagcgg gagcagcaca
     8341 ccatacgtca gtaaggggct gaattctgac cgcattcccg gcactgagcg gcatttgatc
     8401 agcctgcagt actctgacag tgatttcctg ggacaggaac tggtcggtca ggtttactac
     8461 cgcgatgagt cgttgcggta ctacccgttc ccgacggtaa atgcgaataa acaggcgacg
     8521 gctttctcct cgtcacagca ggataccgac cagtacggca tgaaactgac tctgaacagc
     8581 caactgatgg acggctggca aatcacctgg gggctggatg ctgagcatga gcgctttacc
     8641 tccaaccaga tgttcttcga tctggctcag gcaagtgctt ccggagggct gaacaaccat
     8701 aagatttaca ccaccgggcg ctatccgtca tatgacatca ccaatctggc ggccttcctg
     8761 caatccagct atgacattaa tgatattttt accgttagcg gtggcgtacg ctatcagtat
     8821 actgagaaca gggtagatga tttcatcgac tacacgcagc aacagaagat tgctgccggg
     8881 aaggcgatat ctgccgacgc cattcctggt ggttcggtag attacgataa ctttctgttc
     8941 aatgctggtc tgctgatgca catcaccgaa cgtcagcagg catggttcaa tttttcccag
     9001 ggggtggcat tgccggatcc ggggaaatat tatggtcgcg gcatctatgg tgcagcagtg
     9061 aacggccatc tttccctgac aaagagcgtg aacgtcagcg acagtaagct ggaaggcgtg
     9121 aaagtcgatt cttatgaact gggctggcgc tttaccggtg acaacctgcg gactcaaatc
     9181 gcggcatatt actcgctttc caataagagc gtggaaagga ataaagatct gaccatcagt
     9241 gtgaaggacg acaggcgccg tatttacggc gtggaaggtg cggtggacta cctgatcccg
     9301 gatactgact ggagtaccgg tgtgaacttc aatgtgctga aaaccgagtc gaaagtgaac
     9361 ggtcaatggc aaaaatatga cgtgaaggaa tcaagtccat cgaaagcgac agcttacatt
     9421 aactgggcgc cggaaccgtg gagtctgcgt gtacagagca ccacttcttt cgacgtaagc
     9481 gatgcagagg gtaacgatat taatggttac actaccgtcg attttatcag tagttggcag
     9541 cttccggtgg gaacactcag cttcagcgtt gagaacctct tcgaccgtga ctataccact
     9601 gtctggggac agcgtgcacc tctgtactac agcccgggtt acggccctgc ttcactgtac
     9661 gactacaaag gccggggccg aacctttggt ctgaactact cagtgctgtt ctgacgggga
     9721 tgcttttgca acaagggtat gctgtaaaca acatggtcct tggtggccat gttgtttaca
     9781 gccagtataa tatacagcct taacaagata ttccgtctcc ggaatggact gttcaccact
     9841 gaataacatt atttctgttt tatggatctt tgactgaaat ttatcgcata tcctcatttc
     9901 attgtccgtc tgaaaactaa ccttacaagt aagcgtcgtc tcagcaccgt ctggcagatc
     9961 ctgaaattcc tgagaggatg atctg
//
LOCUS       DADLFA010000155         9895 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000155 DADLFA010000000
VERSION     DADLFA010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9895
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.157"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(55..1338)
                     /gene="aroA"
                     /locus_tag="JQH65_003219"
     CDS             complement(55..1338)
                     /gene="aroA"
                     /locus_tag="JQH65_003219"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAY6140542.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1409..2497)
                     /gene="serC"
                     /locus_tag="JQH65_003220"
     CDS             complement(1409..2497)
                     /gene="serC"
                     /locus_tag="JQH65_003220"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HAY6140543.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSIFNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2696..3388)
                     /locus_tag="JQH65_003221"
     CDS             complement(2696..3388)
                     /locus_tag="JQH65_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAY6140544.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            3518..5278
                     /locus_tag="JQH65_003222"
     CDS             3518..5278
                     /locus_tag="JQH65_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HAY6140545.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            5684..6541
                     /gene="focA"
                     /locus_tag="JQH65_003223"
     CDS             5684..6541
                     /gene="focA"
                     /locus_tag="JQH65_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HAY6140546.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            6596..8878
                     /gene="pflB"
                     /locus_tag="JQH65_003224"
     CDS             6596..8878
                     /gene="pflB"
                     /locus_tag="JQH65_003224"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAY6140547.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            9070..9810
                     /gene="pflA"
                     /locus_tag="JQH65_003225"
     CDS             9070..9810
                     /gene="pflA"
                     /locus_tag="JQH65_003225"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HAY6140548.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
BASE COUNT         2534 a         2488 c         2355 g         2518 t
ORIGIN      
        1 atgtgccgac gttttgataa agaaagattt ggctatttat tgcccgttgt tcattcaggc
       61 tgcctggcta atccgcgcca actgctcgaa ataatccgga aatgttttgg ccgtacattt
      121 gggatcaaga atcgtcactg gtgtatctga caacgccacc agcgagaaac acatcgccat
      181 ccggtgatca ttgtatgtcg cgatctcggc aaagttcagt ttttccggtg gagtgatacg
      241 aatgtaatcg tgcccctctt ctacttccgc accgacttta cgcagttctg ttgccatcgc
      301 aaacaggcga tcggtttctt taacacgcca gttatagata ttgcgcagcg tggtggtgcc
      361 ttttgcaaat aacgccgccg tggcaatggt catcgccgca tcgggaatat ggttcatatc
      421 catatcaata gcgttcagtt caccacgcgt gcaggaaata taatcatcgc cccagcaaat
      481 ggtcgcgccc attttttcca gcacatcagc aaagcgaata tcaccctgca tactgttacg
      541 tccaataccg gtcactttta cagtgccgcc tttgattgct gctgctgcca gaaagtaaga
      601 agccgaagat gcatcgcctt cgaccaaata agtacccgga gactgataag actgtccgcc
      661 ttttacgaca aattgttgat agtgctgatt ttcaatttca acaccaaacg tcttcatcag
      721 attgagtgtg atgtcgatat aaggtttaga aaccagatcg cctttaatac gaatcaccgt
      781 atcttccggc gcaagaggcg cagtcattaa cagtgcggtg aggaattggc tggaaacgga
      841 gccatcaacg tcaacgttgc cgccagtaaa gccgccctgt aaacgcaacg gcggataatt
      901 ttcttgttcc aggtaagtga tcttcgcccc gcccaggcgc agcgcatcca ccagatgacc
      961 aatcgggcgt tctttcatac gcggctcacc ggtcagcaca atatcattgc tacccagaca
     1021 aagagctgcc gccagcggac gcattgccgt tccggcgtta ccgaggaaca actccagggc
     1081 accttctgcg tgtaatggac cgccgttacc gataatttcg caacgcgtac gatcggctga
     1141 aagcgtatag cttaccccta acgctgttaa tgcattcagc atatggcgca cgtcatcgct
     1201 atccagcaga ttggttaata ctgttttgcc gtgtgctaat gccgccagca ataaagcgcg
     1261 gttagaaacg ctcttggaac cgggcagatt aatagtgcca tcgacacgag cgatgggttg
     1321 taacgtcagg gattccatga actcaactct ctacaacaga aataaaaacc ccacaggctg
     1381 gctgtgggga ttaagcaaaa tttcggcatt aaccgtgacg gcgttcgaac tcaaccatga
     1441 agtctgtcag cgctttaacg ccttccagcg gcatggcgtt ataaatagaa gcgcgcattc
     1501 cgccgaccac acggtgacct ttcagtgcat gaaggccagc agcaaaagac tcttcaagga
     1561 acaatttgtc aagcgcactg tccgccaact ggaacggcac gttcatccgc gaacggttag
     1621 ctttcgccac gtcattgcgg tagaaatcgc tgttatcaat caccccatat agcagttctg
     1681 ctttttgctg attgatttta tccatttcag ctacaccgcc gttcgctttc agccatttaa
     1741 agaccagacc agatagatac caggcaaatg tcggcggcgt gttaaacatg gagccgttat
     1801 cgttgaagat ggaataatcc agtatcgacg gacacgcgat attcgctttg cccagcaaat
     1861 cttcacgaac gatgacgatt gtcaggccag ccgggccgat atttttctgc gcgccagcgt
     1921 aaatcacgcc ataacggctg acgtcaatcg gacgggaaag aatggttgaa gagaagtcgg
     1981 cggcgaccac cacatctttg ccgaagtctg gcgtttcgtc gatggcgata ccgtcgatgg
     2041 tttcattcgg gcagtaatgc atataagcag cattatcaga gagttgccat tcacgcattg
     2101 gcttaaccgc gcgcagacca tcaacagtca ctttggcgtc aaagacatta ggcgtgcagt
     2161 attttttcgc ttctttaatg gcacttgccg cccagtaacc ggcatcaaca taatctgcgg
     2221 tggttttatc accgagaata ttcagcggta ccgcagcaaa ctgaccgcga ccaccgccat
     2281 ggcagaataa taccttgtag ttggagggga cattaagaag atcgcgaaaa tccttctcgg
     2341 cttcctctgc aacctgaatg aactctttgc cacggtgact cacttccatc accgacgtac
     2401 caagaccgtt ccagtcgcgc agttcctgtt gagcctgttt aagcacctct gccggtagca
     2461 ttgccggacc agaactaaaa ttgaagattt gagccatttc ccctcaccac gttgcgttgc
     2521 tattaacccg cggtcaatcg attgcgacca caggtaatac attattcctg tggctatcgg
     2581 ttttatcatt cagtgacaca catcgcaatg agtaaaaccg ttgagggcaa aaatgaggct
     2641 tgcatcacac aaaagaatct ctcggattat tagcaaaaag ccgatgtttt tactgtcagg
     2701 tcacccgttt tgcccgactt gccttcgtcc attctggatt tgcacagcgc ggacataatt
     2761 gtttttcccc cgctttcata tgaatgattt cactacaacg aatacaggca tagtccgccg
     2821 actccggcac cactttgtaa cgctgcgtac aatcactggg taaaataagt aaacccggtt
     2881 tcaccttgtc atcttcaaag aaatagacac tgatttgccc gttggtttcg agaatcgcca
     2941 gacgtacctg ccccagctgc tccacgccac gcaatcgtag ctccataaag aactcaaatt
     3001 ccgtcatgtt ggagttattg agtttcgacc aggccagctc gccatcttca ataataacaa
     3061 ccggcttacc ttccagtaga tcttccagtt tttcactgtg cgccatcaac cacattacca
     3121 ggcggtataa caacgccaga gtgataaaga cgataagtac cggcaccatc ggcacatcat
     3181 cataaaacgc cacatctccc gccgctgaac ccagcgtgag aatgattaac acttcaaaca
     3241 gcgacatctg tcgcacaccg cgtcttccgg tcattttgag gaacaaaaaa accagtacaa
     3301 aggtataaag actacgtagt gcaacttcgc caaggaaatc aaaaggcact ttatcaaatg
     3361 ccatacggtg gagatcgaac gccttcattt ttatgctctt atcgtggttt attcgctaaa
     3421 agcatagtca acagttcatc ggcatggcgg cggtgatagc gcctttgaga taaaacccgt
     3481 attattgcgc gctttccgta cgactaaagt gattttcatg acgcaaacat ttatccccgg
     3541 caaagatgcc gctctggaag attccatcgc tcgcttccag caaaaacttt cagacctcgg
     3601 ctttcagatt gaagaggcct cctggctgaa tcctgtgcct aacgtctggt ctgtacatat
     3661 tcgcgacaaa gagtgcgcac tgtgttttac caacggtaaa ggcgcaacca aaaaagcggc
     3721 gctggcttct gcacttggtg aatatttcga gcgtctctca accaactact tttttgctga
     3781 tttctggctg ggcgaaacca tcgccaacgg tccgttcgtg cattatccca acgaaaaatg
     3841 gttcccactg accgaaaatg acgatgtgcc agaaggacta ctcgatgacc gtctgcgcgc
     3901 gttttatgat ccggagaatg aactgaccgg cagcatgctg attgacctac aatccggtaa
     3961 cgaagatcgt ggtatttgcg gcctgccgtt tacgcgtcag tccgacaatc agaccgttta
     4021 tattccgatg aatatcattg gtaacctgta cgtctccaac ggtatgtccg caggtaatac
     4081 ccgcaacgaa gcacgcgttc agggattgtc tgaagttttc gaacgctacg tgaaaaaccg
     4141 cattattgct gaaagcatca gcctgccaga gatcccggca gacgtgctgg cgcgttaccc
     4201 agcggtggtt gaagcgatcg aaacactgga agcagaaggt ttcccgatct tcgcatatga
     4261 tggttccctt ggcggccagt atccggtgat ttgcgtggta ctgttcaatc ctgctaacgg
     4321 tacctgcttt gcctctttcg gtgcgcatcc tgattttggc gtagcactgg aacgtaccgt
     4381 gaccgagctg ctgcaaggtc gtggcctgaa agatttggat gtgtttactc cgccaacctt
     4441 cgatgatgaa gaagtcgctg aacataccaa cctcgaaacg cactttatcg attccagcgg
     4501 tttaatctcc tgggacctgt tcaagcagga tgccgattat ccgtttgtgg actggaattt
     4561 ctccggcacc acggaagaag agtttgctac gctgatggct atcttcaaca aagaagataa
     4621 agaagtttat attgccgatt acgagcatct gggcgtttat gcttgccgta ttatcgtgcc
     4681 tggcatgtcc gatatttatc cggctgaaga tctgtggctc gcgaataaca gtatgggcag
     4741 ccatttacgt gaaacgattc tttcgctacc aggcagcgag tgggaaaaag aagattacct
     4801 gaacctcatc gagcaactgg atgaagaagg ttttgatgac tttacccgcg tgcgtgagct
     4861 gttgggtctg gcgaccgggt cggataacgg ttggtacacc ctgcgtatcg gtgaattaaa
     4921 agccatgctg gcgctggctg gtggcgatct ggaacaggct ctggtctgga ccgaatggac
     4981 gatggagttt aactcatcag tatttagtcc ggaacgcgcc aactattatc gctgcctgca
     5041 aacgttgtta ttactggcac aggaagaaga tcgccagccg ctgcaatatc tgaatgcgtt
     5101 tgttcgcatg tacggcgcag atgccgtgga agccgccagt gcggcaatga gcggcgaagc
     5161 ggcgttttac ggcctgcaac cggtagatag cgatctgcac gcgtttgctg cacatcagtc
     5221 gttgttgaag gcctacgaaa agctgcagcg cgccaaagca gcattctggg caaaataaaa
     5281 tcaaatagcc tacgcaatgt aggcttaatg attagtctga gttatattac ggggcgtttt
     5341 tttaatgccc cgctttacat atatttgcat taataaaata attgtaatta taaggttaaa
     5401 tatcggtaat ttgtatttaa taaatacgat cgatattgtt actttattcg cctaatgctc
     5461 ccttttaatt aactatttta gcggaggatg cggaaaaaat tcaactcatt tgttaatttt
     5521 taaaatttat ttttatttgg ataatcaaat atttactccg tatttgcata aaaaccatgc
     5581 gagttacgga cctataagcc aggcgagata tgatctatat caatttctca tctataatgc
     5641 tttgttagta tctcgtcgcc gacttaataa agagagagtt agtgtgaaag ctgacaaccc
     5701 ttttgatctt ttacttcctg ctgcaatggc caaagtggcc gaagaggcgg gtgtctataa
     5761 agcaacgaaa catccgctta agactttcta tctggcgatt accgccggtg ttttcatctc
     5821 aatcgcattc gtcttctata tcacagcaac cactggcaca ggcacaatgc ccttcggcat
     5881 ggcaaaactg gttggcggca tttgcttctc tctggggctg attctttgtg ttgtctgcgg
     5941 agctgatctc tttacttcca ccgtgttgat tgttgttgct aaggcgagtg ggcgcatcac
     6001 ctggggtcag ttggcgaaaa actggctaaa tgtctatttt ggcaacctgg tcggcgcact
     6061 gctgtttgta cttttaatgt ggctttccgg cgagtatatg accgcaaatg gtcaatgggg
     6121 actaaacgtc ctacaaaccg ccgaccacaa agtgcaccat acttttattg aggccgtctg
     6181 tcttggtatc ctggcaaacc tgatggtatg tctggcagta tggatgagtt attctggccg
     6241 cagcctgatg gacaaagcgt tcattatggt gctgccggtc gcgatgtttg ttgccagcgg
     6301 ttttgagcac agtatcgcaa acatgtttat gatcccgatg ggtattgtaa tccgcgactt
     6361 cgcatctccg gaattctgga ccgctgtcgg ttctgcaccg gaaaattttt ctcacctgac
     6421 cgtgatgaac ttcatcactg ataacctgat cccggttacg atcggtaata ttatcggcgg
     6481 tggtttgttg gttgggttga catactgggt catttacctg cgtgaaaacg atcaccatta
     6541 atggttgtcg aagtacgcag taaataaaaa atccacttaa gaaggtaggt gttacatgtc
     6601 cgagcttaat gaaaagttag ccacagcctg ggaaggtttt accaaaggtg actggcagaa
     6661 tgaagtaaac gtccgtgact tcattcagaa aaactacact ccgtacgagg gtgacgagtc
     6721 cttcctagct ggcgctactg aagcgaccac caccctgtgg gacaaagtaa tggaaggcgt
     6781 taaactggaa aaccgcactc acgcgccagt tgactttgac accgctgttg cttccaccat
     6841 cacctctcac gacgctggct acatcaacaa gcagcttgag aaaatcgttg gtctgcagac
     6901 tgaagctccg ctgaaacgtg ctcttatccc gttcggtggt atcaaaatga tcgaaggttc
     6961 ctgcaaagcg tacaaccgcg aactggatcc gatgatcaaa aaaatcttca ctgaataccg
     7021 taaaactcac aaccagggcg tgttcgacgt ttacactccg gacatcctgc gttgccgtaa
     7081 atctggtgtt ctgaccggtc tgccagatgc atatggccgt ggccgtatca tcggtgacta
     7141 ccgtcgcgtt gcgctgtacg gtatcgacta cctgatgaaa gacaaactgg cacagttcac
     7201 ttctctgcag gctgatctgg aaaacggcgt aaacctggaa cagactatcc gtctgcgcga
     7261 agaaatcgct gaacagcacc gcgctctggg tcagatgaaa gaaatggctg cgaaatacgg
     7321 ctacgacatc tctggtccgg ctaccaacgc tcaggaagct atccagtgga cttacttcgg
     7381 ctacctggct gctgttaagt ctcagaacgg tgctgcaatg tccttcggtc gtacctccac
     7441 cttcctggat gtgtacatcg aacgtgacct gaaagctggc aagatcaccg aacaagaagc
     7501 gcaggaaatg gttgaccacc tggtcatgaa actgcgtatg gttcgcttcc tgcgtactcc
     7561 ggaatacgat gaactgttct ctggcgaccc gatctgggca accgaatcta tcggtggtat
     7621 gggcctcgac ggtcgtaccc tggttaccaa aaacagcttc cgtttcctga acaccctgta
     7681 caccatgggt ccgtctccgg aaccgaacat gaccattctg tggtctgaaa aactgccgct
     7741 gaacttcaag aaattcgccg ctaaagtgtc catcgacacc tcttctctgc agtatgagaa
     7801 cgatgacctg atgcgtccgg acttcaacaa cgatgactat gctatcgctt gctgcgtaag
     7861 cccgatgatc gttggtaaac aaatgcagtt cttcggtgcg cgtgcaaacc tggcgaaaac
     7921 catgctgtac gcaatcaacg gcggcgttga cgaaaaactg aaaatgcagg ttggtccgaa
     7981 gtctgaaccg atcaaaggcg atctcctgaa ctacgatgaa gtgatggagc gcatggatca
     8041 cttcatggac tggctggcta aacagtacat cactgcactg aacatcatcc actacatgca
     8101 cgacaagtac agctacgaag cctctctgat ggcgctgcac gaccgtgacg ttatccgcac
     8161 catggcgtgt ggtatcgctg gtctgtccgt tgctgctgac tccctgtctg caatcaaata
     8221 tgcgaaagtt aaaccgattc gtgacgaaga cggtctggct atcgacttcg aaatcgaagg
     8281 cgaatacccg cagtttggta acaatgatcc gcgtgtagat gacctggctg ttgacctggt
     8341 agaacgtttc atgaagaaaa ttcagaaact gcacacctac cgtgacgcta tcccgactca
     8401 gtctgttctg accatcactt ctaacgttgt gtatggtaag aaaactggta acaccccaga
     8461 cggtcgtcgt gctggcgcgc cgttcggacc gggtgctaac ccgatgcacg gtcgtgacca
     8521 gaaaggtgct gtagcgtctc tgacttccgt tgctaaactg ccgtttgctt acgctaaaga
     8581 tggtatctcc tacaccttct ctatcgttcc gaacgcactg ggtaaagacg acgaagttcg
     8641 taagaccaac ctggctggtc tgatggatgg ttacttccac cacgaagcat ccatcgaagg
     8701 tggtcagcac ctgaacgtta acgtgatgaa ccgtgaaatg ctgctcgacg cgatggaaaa
     8761 cccggaaaaa tatccgcagc tgaccatccg tgtatctggc tacgcagtac gtttcaactc
     8821 gctgactaaa gaacagcagc aggacgttat tactcgtacc ttcactcaat ctatgtaatt
     8881 agatttgact gaaatcgtac agtaaaaagc gtacaataaa ggctccacga aagtggggcc
     8941 ttttttagca cgagagcctt ttttgtcagc tatctatact ttaaggtgac tgccaaaaca
     9001 gactcgacgt agccttcgag ctgcgcacca acacggcctc agatgggcca catctggaga
     9061 aacaccgcaa tgtcagttat tggtcgcatt cactcctttg aatcctgtgg aaccgtagac
     9121 ggcccgggta ttcgctttat cacctttttc cagggctgcc tgatgcgctg cctgtattgt
     9181 cataaccgcg acacctggga cacgcatggc ggtaaagaag ttaccgttga agatttgatg
     9241 aaggaagtgg tgacctatcg ccactttatg aacgcttccg gcggcggcgt taccgcatcc
     9301 ggcggtgaag caatcctgca agctgagttt gttcgtgact ggttccgcgc ctgcaaaaaa
     9361 gaaggcattc atacctgtct ggacaccaac ggttttgttc gtcgttacga tccggtgatt
     9421 gatgaactgc tggaagtaac cgacctggta atgctcgatc tcaaacagat gaacgacgag
     9481 atccaccaaa atctggttgg agtttccaac caccgcacgc tggagttcgc caaatatctg
     9541 gcgaacaaaa atgtgaaggt gtggatccgc tacgttgttg tcccaggctg gtctgacgat
     9601 gacgattcag cgcatcgcct cggtgaattt acccgtgata tgggcaacgt tgagaaaatc
     9661 gaactcctcc cctaccacga gctgggcaaa cacaaatggg tggcaatggg tgaagagtac
     9721 aaactcgacg gtgttaaacc acctaagaaa gagaccatgg aacgcgtgaa aggcattctt
     9781 gagcagtacg gtcataaggt aatgttctaa tttccccgta aagcggcaac tttattgagt
     9841 tgccgctttt ttattccgcg cttcattctg ctacagtgtt tttaacacat tttat
//
LOCUS       DADLFA010000156         9779 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000156 DADLFA010000000
VERSION     DADLFA010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9779
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.158"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(188..529)
                     /locus_tag="JQH65_003226"
     CDS             complement(188..529)
                     /locus_tag="JQH65_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HAY6140549.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVEGLKL
                     IESGDWLDRDK"
     gene            complement(532..4311)
                     /gene="tamB"
                     /locus_tag="JQH65_003227"
     CDS             complement(532..4311)
                     /gene="tamB"
                     /locus_tag="JQH65_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HAY6140550.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFSR
                     PVLPEMTDLHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVTQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTLVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDFAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVTFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVLVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPEQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(4308..6041)
                     /gene="tamA"
                     /locus_tag="JQH65_003228"
     CDS             complement(4308..6041)
                     /gene="tamA"
                     /locus_tag="JQH65_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HAY6140551.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSCGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            6247..6876
                     /gene="msrA"
                     /locus_tag="JQH65_003229"
     CDS             6247..6876
                     /gene="msrA"
                     /locus_tag="JQH65_003229"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAY6140552.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNEPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGVCLPPEA"
     gene            7199..8542
                     /locus_tag="JQH65_003230"
     CDS             7199..8542
                     /locus_tag="JQH65_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAY6140553.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(8604..8810)
                     /locus_tag="JQH65_003231"
     CDS             complement(8604..8810)
                     /locus_tag="JQH65_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HAY6140554.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            9135..9689
                     /locus_tag="JQH65_003232"
     CDS             9135..9689
                     /locus_tag="JQH65_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HAY6140555.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
BASE COUNT         2257 a         2682 c         2404 g         2436 t
ORIGIN      
        1 tactcaccgt tcaaagcgtg cccccgtttt acccctgcac atttgtgaac gccagcagag
       61 ttttttatgc gattgcagat atggaaagcg gctcggagag aaaagcaaaa gatggaaaag
      121 ttacaaaaaa aaatcgcgag atacatcgca taaaacaacg ccacccgaag gtggcgtgct
      181 tttattatta cttatccctg tctaaccagt cgccgctttc aattagcttt aatccctcga
      241 cgggtcgttg ataaacgtac atccatgcac tcccgtacgg cgtctgaatc aactggcgcg
      301 cgtattcacc gcccctggtg cgcaaggcat caagttcggc cagcgtggcg ttgtcaatac
      361 gataaacttc accgtgtacc gttccgttcc ccggaactgc gcctggatag tggcccaggc
      421 tatacaactg gtagttatcg atactgaaat cgcccagtaa ctgggcattg gtcatccagt
      481 gactgttgcc ttgtttgtgg cgtaaactgc cgtagacaaa tattcgcatt gctaaaactc
      541 gaactgatag agcaaatcca gtgcctggtc tacaccagac acggcttcca gatatagctt
      601 aggcatcagg cgataacgta acgtgagtgt tgctatagag tcaaatatac ccacgccgta
      661 tttcacttgc aggcctggca atacatagcc gctgaccact acctgggagg agtcgcctac
      721 tccctgggtg tcgagcgcta aattgcttac gccaaacgtc tcgccgattt tacccacaat
      781 ctggccactt tgcgcaaccc ccagaccaat cagcatcgag gtcattgccg cactgtcgct
      841 ctgatcgctc tccagccctt gtccacgtag caaataagac aatgcagctt gttgcgacat
      901 cgccgggtca gagaagatct ccgctttcgg ttcgtccgcc agaccagtga cgcgaactcc
      961 ggcgattacg tcgtcttctg tagcatccgg gttacgaata gcttcaatat taagatacgg
     1021 ttgctctggc ggaccagaga acagtagctc acccttacgc acaatcagat cctgaccata
     1081 ggcatggaag cgcccttcag ggatgttgat ctgcccgttc aggcccagcc cttgtttgtc
     1141 ctgaacgacg ttaagatcgc ccgtcagccg cgctttcagg ccaaaggcgt caatgcgcac
     1201 attgttgcca acgtggacaa tcaggttact gttaatcgga atcgacgccg ttttcggctc
     1261 ttccggttgc aggttatcgt taagcatcac cacatcgctg gagacgccta ctgcgctttc
     1321 cggcagatcg tgcaccacga tgcgcgccca cgggacatcc acgcgaccat cgagggtaaa
     1381 caggtttggt gtagcctcga atacaacatc tggcgataca tccattcgta ccatcggcgg
     1441 cacggtgatc cgcactttac tgcctttcgc cgttactcgt gcccgccagt tttcaatttg
     1501 gctccagtcg gcatcaccgt tcaggtagat ttcaccctgc tgggtccgta ctgtaccggc
     1561 aagcgtcgag cgcataccgt taaagttgac cgcaagctgg ctcggctgca tatcaaacgg
     1621 cataaagttg ccgtcgatat ccacacccgt aacctgaagc tgaccaaaca actgcgggct
     1681 ttgcacatca ccacccagac gcaagttggc actcaccatc cccgctgctt tttccccacg
     1741 agtaaagatg gggtttatca tcgcaaggtt gaagttacgg atattgacgt tgccaccaag
     1801 attacggcgg ccttgcggat cggtcaccag cacctgtcca tcaaactggc cgttattggt
     1861 caggcggatg gtccagccca attcggcacg gttgttacgc aattccgccg tcagattcag
     1921 tgtctgaaac gtcaccggca gcgccgcatc gttgacggtt tgcgttacct gcacgttacg
     1981 ccccgaaagg gtgatactgc cctgcggcag cccctctttc gtggtgtccc aggcaacatc
     2041 cgctttaccc gtgaagatac cgctggcctg agtggtttct ggcataaacg gtttcagcat
     2101 ggcgaagtcg aagcggttga gattcaccac cgcacgccct tcggcccccg catcgatagt
     2161 ttgcggcacg cacagttccg cattcgggtt aagccagcag tgtggcccga tgctgatttt
     2221 ttgctcctta ttgcggtaat ccagcgcaat atcgcgggtc agcgaccacg ggccgaccag
     2281 cgtctggaag cgggtattgc taagagttcc cttccagcgt tcttctttgc gatcaaaact
     2341 tcctgccaga ttaagctgcc cggagacagg ctcgccctga atccgcaact gtagctcgtg
     2401 ctgcttttcg ctgcctttgg cattcagggt gacgaggttg atatttacat ccggctgcga
     2461 aatttgctca acgcgtacgt cgagtttccc ggcgatctga tcggtggatt taatgtcgcc
     2521 ttccacgcga acctgcgtca cggaaagttc ctgccagcgc aggccgcgcg cggtgatatc
     2581 tgccagtagt tgtggcgctt ccaccgtgcc gcgtactttc accagccctt tcgctgtacc
     2641 gccaagcccc ggcagcgcgt tatccagccc cggcgcgttg atggtggcat caagattgag
     2701 atcttttacc cccagctcgc ctttcacttc ggcactgttt ggccccagtt ccagatgaag
     2761 cccagggatc atccactgca tataactgtt gcctttcagc gtgccgtcaa cgttcacttt
     2821 gttctgtttg acgttaccgg tcagcttcag ttctggcacc tccatctgcc aggtgccgcc
     2881 gtacaggcta ccgcgggttt taatcaagcc attgagtttc gacggccact ccgggaactc
     2941 tttggcggtg ttaatgccat taagcgttag ctcaccgcgc caactaatgg cctgctgcca
     3001 gtcgagcaac gccttgagtt cagttttccc ttccagcgcc gcgacggtga gtttgtcgag
     3061 attgacctgc tgttcattac ctttggcatc aagggtaatg gtggctggcg ggatctcctg
     3121 tcccttcact gccgtacgca tagagagcgt gtaatcggtc attttgccgg taagtttcag
     3181 tttcagatca tccgcctgat actgcttctc accagtgaac ggccagtaaa gctgtttgct
     3241 gttcacttcc acgttgagcg gcaatccggc ttccgccagt cgcgtctggg cgcgtaaatc
     3301 catatccacc ggaccggaaa gattaacgcc aatctccagc tgttcgcgca gcgcgccgcc
     3361 cacttttagc ttcacttttt cacctttcaa cggctccacg ttcagtgtgc tgttgagggt
     3421 gatatccacc ggccagttgt ctgacagctg cgccgtgccg ctggcgttga cgatcccctg
     3481 gttggaatcg atatccaggg cgtccagttt agtattgccg tcaatgctgc tcactttcag
     3541 cagcatggtg cgcacggtga tgtccgtgtc gcccgtcacg cgtaactgct cgcctttaaa
     3601 ctcttcaatg ttcaggttaa gcggcaaatg caagtcggtc atttccggca ataccgggcg
     3661 agaaaagaga tctttcagcg tttcgccgag cggcttttca tccggctgcg gattttcaat
     3721 tttcggttcg accacttctt cctgcgccac ttccgccact ttcggcagag caatcagcag
     3781 gcctttcagc gacgtcggtt tcagggtcag ggttttctcc tgccagttca ggccggaggt
     3841 gaagtccatc accgacaccg tggtgtcatc aatcttgatg ttgacgttgt ccagcgccac
     3901 ccgtgtcagg gtgatgggat acggcgtgga gagatccagc ggaccgctat cttcctcttc
     3961 ttcaacctgt tcagaaggag gcattttttt actgtcgatg ttgacctgaa tgtctttcag
     4021 cgccaggtca ttaatacaaa cgctactgtt ccacaggcac tcaagaccga ccgccagatg
     4081 cagattaccc gcttttaccg ccacgcctgg ctgctcataa cgaacgtcag acaaggtgag
     4141 atcgcgccag ccgccggtga ccttgccaat atccagtcct ggcacccagc gatccgccgc
     4201 tttaaataca agatgcaggc cgctggtggt gcccaccaga aacgccaccg atcccagcaa
     4261 cagtaagata acgataacca cgccgaggct gatttttttc cataaactca taattctggc
     4321 cccagaccga tgtaaaactg taacccgtgt tcgtctttat ccgcgaccgg tacggcaaaa
     4381 tcgagtttga ttggcccgac cggcgattcc cagcgcacgc cgaccccggt accggtttta
     4441 aagtcgctgc ggcgaatatc gcttaccgct tcgccactat cgacaaacac cgcgccccac
     4501 cattttccgg tcacgttgta ctggtattcc agcgatccgg ttatcaactt cgaggcccct
     4561 ttcaggtcac cgttggcgta tttcggggcg atagatttgt atttgtagcc gcgaatactg
     4621 cgatcgcccc cggcgaaaaa acgcagatcc ggcggtactt tgtcgaaatc accggtttca
     4681 atccagccca gcgtgccgcg tgtaacgaaa cgatggcgat cgtacagtgt gcggatccag
     4741 acgttctgcg cctggaaaac ggagaaatcg acatctgagc cccaggccgt gttggagtag
     4801 tcgatagagt agcgttgcga gtcgccccag gttggcatca ggccaccaca agaacgcgtg
     4861 cggctaatca tcaccccagg ataaaacagc atcgtggtgt tggtaatttc accctgagta
     4921 aagtggtcga gactccagcg caggttaatg gcacgctgcc aaccgctgga gagatcccag
     4981 tagcgagaag ccaccagcgt agtggagtca gattcggtat cgttcaggtc agtgcgctta
     5041 aaaccgccct gcaccaaata atattgttcc agtggattct tcagcagcgg cattttatag
     5101 ctgaagtcga gggtctgttc cggcgcggaa atactggtac tggtggtcaa actgtgaccg
     5161 tatgagttca tccacggctt tttccacgtc gctttcacgc gcggtcccac gtccgtagag
     5221 taaccgaccc cggtttcgat ggtgttttct gttcgcggcg aaaccacgcc cgtcaatggt
     5281 aatactttcg tttcgcgcgc tttatcaaat tgtggagcca ccaccaccga gttaaaccag
     5341 ccggtagcag aaagtcggcg gttcagttcc gccagatctt tcgattcata ctcatcgccc
     5401 tctttaaacg gcaccagatt ttgcaggtat tcatcgcgga tttgtgatcc ttcaaaggtc
     5461 acatgcccaa agcggtaacg ttcgccactg ttataatcaa tatcccagaa ggctttatgc
     5521 aggccgagcg caatgcccag ctgcgctttg gtaaattcgc tatcgaaata acctttacgc
     5581 aacgcaatac tggttaagga ctttttgaaa ttttcataat cgccctggtt cagtaccgta
     5641 ccaatagccg ggcgagtatc gagcaatttc aaatagtctt tatcggtccg cgcgccgccg
     5701 cgcaatacca catcggtgcc gccaattaac accggcacac ctggcgtgac tttggcgatc
     5761 aatacctgcc gccctttctt tggcggtgga cggagatcaa attcaatggt cggctggtaa
     5821 taacccagcg ctttcagacc ttcacggatg gcatcatcga cgcgtgcgcg aaagcggcgg
     5881 tctggcgtca cttcatcact ttcaatcgta gaaagctgcg cacgaacgtt cttttccagc
     5941 tgtcccgata acccctcgac ctgtagacgg acgttcgcgg cgacggcaga tccgcttaag
     6001 cagagtaagc ttacacagca taactgtcgg atatagcgca cattttctcc tgaatatcct
     6061 tttttcctgc ccctggaacg ccgttaaacg gcttaacaaa aatccagtaa tatggattaa
     6121 aaagcagact aaaccccaaa tatttcttat gttttacttt agacctattc acggtggtta
     6181 ttgtgtgcaa atacgcctct tgttacaacc ttaaccccaa tgaccgattt tcgggagagc
     6241 gacaccatga gtttatttga taaaaagcat ctggtttccc ccgccgatgc cctgcctgga
     6301 cgtaacaccc cgatgcccgt agccacgctg catgcggtca acggtcactc aatgaccaat
     6361 gaacctgacg gaatggagat tgccattttt gcgatgggtt gtttctgggg tgtggagcgt
     6421 ctgttctggc agttacccgg cgtttacagc accgccgcag gctataccgg cggctatacg
     6481 ccaaatccga cttatcggga agtgtgctcc ggtgatacgg gtcatgccga agcggtacgc
     6541 attgtttacg atccttccgt catcagctat gagcagttgc tacaggtatt ttgggagaat
     6601 cacgatcccg cccagggaat gcgtcagggc aatgaccacg gcacgcagta tcgttcagcg
     6661 atttatccgc tgaccccaga acaggatgcc gcagctcgcg ccagtctgga acgttttcag
     6721 gcggcgatgc ttgccgccga tgacgatcgt cacatcacca cggaaatcgc taacgccaca
     6781 ccgttttatt atgccgaaga tgaccaccag caatatctgc ataaaaaccc atatggttac
     6841 tgtggaattg gcgtctgtct gccgccggaa gcatagcgtt acgggtacaa atgtagtttg
     6901 ttgataaagt gctctttgtc tatgcctgat gcggcttaaa cgacttacca ggcctacaaa
     6961 atcgtccaaa ttcaatatat tgcaaggact gcgtaggcct gatcggcata gcgcatcagg
     7021 cagtattgca tttatcagca gtctgaattt taaccctctg gcgactttac agtaccttac
     7081 gctatactag ccactgaaaa ttccggttca ctttcttcga atcggctttc aatgtgtatt
     7141 tcacacaaat taatcaactt tcccttccga ggatctggcc tgaaaggtcg gataagatat
     7201 gttaaacagt attttagtca tactctgctt gatcgctgta agtgcgttct tctcgatgtc
     7261 cgagatctcg cttgccgcct cacgcaaaat caaacttaaa ctgctggctg atgaaggcaa
     7321 tataaatgcc caacgcgttc tgaatatgca ggaaaatccc ggcatgttct ttaccgtggt
     7381 ccaaatcggt ctgaacgcag tggcgattct cggcggtatc gtcggtgatg cggcattttc
     7441 tccagctttt cacagcctgt tctcccgcta tatgtcggca gagctctctg agcaactgag
     7501 ctttattctc tctttctcgt tagtgactgg catgtttatc ctgtttgcgg atttaacccc
     7561 gaaacgcatc ggtatgattg cgccagaagc ggtggctttg cgtatcatca acccgatgcg
     7621 cttctgcctg tacgtttgca ccccgctggt gtggttcttc aacggcctgg cgaacatgat
     7681 cttccgtatt ttcaaactgc caatggtacg taaagatgac atcacttctg atgacatcta
     7741 cgcggtagtg gaagccggtg cgctggcggg cgtgttacgt aaacaggaac acgagctgat
     7801 tgaaaacgtc tttgagctgg aatcccgtac cgttccgtct tcaatgacac cgcgtgaaaa
     7861 cgtgatttgg tttgatctcc acgaagatga gcaaagcctg aagaataagg tggcggaaca
     7921 tccgcactct aagttcctcg tctgtaatga agatattgac cacatcatcg gttatgtcga
     7981 ttctaaagac ctgctgaacc gcgtgctggc taaccaaagc ctggcactga acagcggcgt
     8041 acaaattcgc aacacgctga ttgtgccgga tacgttaacc ctttcagagg cgttggaaag
     8101 ttttaaaacc gcaggtgaag acttcgcggt gatcatgaac gagtacgcgc tggtggtggg
     8161 gatcatcacc ctcaacgacg tgatgaccac gctgatgggc gatctggtcg gtcaggggct
     8221 ggaagagcag attgtcgccc gtgatgagaa ctcatggctg attgacggcg gcaccccaat
     8281 tgacgacgtc atgcgcgtgc tggatattga cgagttcccg cagtcgggca actacgaaac
     8341 catcggcggc tttatgatgt ttatgctgcg taagatcccg aaacgcaccg attcggtgaa
     8401 attcgccggc tacaaatttg aagtggtgga tatcgataac taccgcatcg accagctgct
     8461 ggtgacccgg atcgacagca aggccaccgc cctttcgcca aaactgcctg acgctaaaga
     8521 taaagaagaa agcgtcgcgt aacccccaga aacatcaacg gctcctgaat caggagccgt
     8581 cttattactg catagcactt tggttaagcc atctctgttt gcagacgcat aacctgacgg
     8641 ttaacttcgg acatcactga caaatgcagc ttatccttca cttttgggat aagaatctta
     8701 cccttatcaa attcaaaagc gccaacgtcc ttgatgtata accgtccacg gaacaggatc
     8761 tttacgtact tcgccacctg aagtgggttg taccgttgga aaattttcat tgttatctcc
     8821 tgctgagtat tacgcctgtg cggtgccaca atcggcccaa ctattatgag acgcaaattt
     8881 taatgcttag tgactataga ctatccgggc aatgtttcca ccgcgtataa ctttttttac
     8941 ctaaaggtta caattattca gaattatctt tttaccgaag cgcggtcttc agtataagca
     9001 ttcatttttc atatgatttg tgcgcttgac cgcaaactgg catcacactt gcgggaaatt
     9061 cgataaatag cacatatgat taaaactcag atccaagtgg tcggatcacc tgcatatcat
     9121 aagaaggaaa caccatgacc ctacgcaaga ttctggcact cacctgcctg ctgttgccga
     9181 tgatggcttc cgcacatcag ttcgaaaccg gtcagcgagt gccgccgatt ggcattaccg
     9241 atcggggcga gttggtgctt gataaagatc agtttagcta caaaacctgg aacagcgcgc
     9301 agttagtggg aaaagtgcga gtactgcaac atattgctgg tcgcacctct gcaaaagaga
     9361 aaaacgcgac gctgattgaa gcgattaaat cagcaaagtt accgcacgat cgttaccaga
     9421 ccaccaccat tgttaacacc gacgacgcaa ttccgggttc cggcatgttt gtgcgcagca
     9481 gtctggagag taataaaaag ctttatccct ggtcgcagtt tattgtcgat agcaatggcg
     9541 tcgcgcgcgg tgcctggcag ctggatgaag aaagttccgc tgtagtggtg ctggataagg
     9601 atggtcgcgt gcaatgggcc aaagacgggg cgctcacaca ggaagaggtg cagcaagtga
     9661 tggacctgct gcaaaagttg ctcaaataat ggcaataaca taaattctgc aaaaataatt
     9721 ttcccctatc gttatctatg ggatagggga attaggtctc tgagctctta caccgaact
//
LOCUS       DADLFA010000157         9654 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000157 DADLFA010000000
VERSION     DADLFA010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9654
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.159"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(1..1094)
                     /gene="fdeC"
                     /locus_tag="JQH65_003233"
                     /pseudo
     CDS             complement(1..1094)
                     /gene="fdeC"
                     /locus_tag="JQH65_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308363.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like adhesin FdeC"
     gene            1647..1736
                     /locus_tag="JQH65_003234"
                     /pseudo
     CDS             1647..1736
                     /locus_tag="JQH65_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
     gene            2210..2311
                     /locus_tag="JQH65_003235"
     CDS             2210..2311
                     /locus_tag="JQH65_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6140556.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
     gene            2672..2938
                     /locus_tag="JQH65_003236"
     CDS             2672..2938
                     /locus_tag="JQH65_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAY6140557.1"
                     /translation="MMKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYP
                     YVTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGS"
     gene            2938..3078
                     /gene="rpmJ"
                     /locus_tag="JQH65_003237"
     CDS             2938..3078
                     /gene="rpmJ"
                     /locus_tag="JQH65_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAY6140558.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(3113..3340)
                     /locus_tag="JQH65_003238"
                     /pseudo
     CDS             complement(3113..3340)
                     /locus_tag="JQH65_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4163..4705
                     /gene="ecpR"
                     /locus_tag="JQH65_003239"
                     /gene_synonym="matA"
     CDS             4163..4705
                     /gene="ecpR"
                     /locus_tag="JQH65_003239"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HAY6140559.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLSEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMQPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKIYKLVQ"
     gene            4731..5366
                     /gene="ecpA"
                     /locus_tag="JQH65_003240"
                     /gene_synonym="matB"
     CDS             4731..5366
                     /gene="ecpA"
                     /locus_tag="JQH65_003240"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HAY6140560.1"
                     /translation="MTHWDITSSIQLGKKCNEKKVLAIALVTVFTGMGVAQAADVTAQ
                     AVATWSATAKKDTTSNLVVTSLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKL
                     TSRLITNTLTQLDTSGSTLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYN
                     ASNRTTAQDGFTFTIISGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            5424..6092
                     /locus_tag="JQH65_003241"
     CDS             5424..6092
                     /locus_tag="JQH65_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HAY6140561.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            6118..8641
                     /locus_tag="JQH65_003242"
                     /pseudo
     CDS             6118..8641
                     /locus_tag="JQH65_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414825.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8631..>9654
                     /gene="ecpD"
                     /locus_tag="JQH65_003243"
     CDS             8631..>9654
                     /gene="ecpD"
                     /locus_tag="JQH65_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HAY6140562.1"
                     /translation="MRVNLLITMIIFALIWPVTALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYTGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYMQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSLSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNL"
BASE COUNT         2628 a         2369 c         2255 g         2402 t
ORIGIN      
        1 gcaatacgga tcacttcaga tttgcggtac tcaagaacga tgttgttatt acgctcaacc
       61 aggtcatagc ggctgcctgc cagcattcga cgctcgcgaa tgctgtctgt atcgagttgt
      121 ttctccagag gttcgccaat ccgataatta acttccaggc caaagcgagt gtcattctca
      181 ccactcttgc cctgcttatg cccggcactc agggtcagaa gaggcacagg cgtgtaattc
      241 acttcagcgg aaatcgcatg tggatctttc tggcgtttat ctttgccaaa cagcccaact
      301 tcatcgccat aatactgctc atacatcagg cttgcgccaa gctgcggtca ggcgggtaaa
      361 tagccctcag cacgaatatc ccagccattc gccgggcgtt cctgataatc ctcaatatcc
      421 ggcgattttt tccagccaga agcccgaata taaccattgg cgctcagttt cagataatcg
      481 cgccagtatt ccgcaccaac accaatgcgg gtatgactac gggataaatc atggtcgata
      541 aaggtgttca ccccccccat tcagtcattt cctgaaaaat gacgccagcc aaaaccaata
      601 tttgactgag tacgatcgtc tgtacgatgt attgccccct gagtgaacaa catatttgtc
      661 ggcgtatcat aaatcggata aagcatttcc agcgaagaat ccttcagcga gaaatcttta
      721 tcgacattca gtttgacgcg cgctgtacca tatttcccga gccactcctg tatttcctgg
      781 ttagctttag cggtggccat tccggtaata aaattacgtg tcgcatcgct atctggctga
      841 ctgcttaaaa atgtcccggc atttgcggca aacgacgcga catttttctc cacgttatta
      901 tcagcagtta ccgtagtatt tcccatgctc aaccgtggct gaaccgcatg ctgcgcacgt
      961 gccgccatta ctggggtaaa ggtgacagcg agtggaaaaa gaacctgaac agagatattt
     1021 gcccacgcca cgcagcgggc cagaactgaa taacgaaatc gtggttgttt atgacctgtt
     1081 ttataacgtg acattgagat aatttcctga tgaacgaatt aacgaacata ggcagacaca
     1141 aataatggcg tctgcttgtc agaaaaaccg gggatttccc ggtttccttt tccgcagaaa
     1201 aggaaatacc tttatgtaag aaagtgcacc gccacctgat atatccagtg tctgtagcca
     1261 ctcatcaggt ggttgggcgc gggattatat ttttatttat gggtaaaaat ttagcttttt
     1321 gtgcaagtca ttaaatagat acctaagtta tatttcttac tctgcggaca gtgaataatt
     1381 cattattcag actgaataat aatgacctgc ctttaattag caactattgg aaatacgtcg
     1441 aagtaggtat atacgccagg ataaaccgat atatttatta tggatattca ttttccttca
     1501 tcggactgta aattgcgggg atatcgaact tacactactc atcgcaacac tcaaacagat
     1561 ttatgaattt cgttcataac ccctgacaaa tcctgccgtc taatcgagct tttcccttta
     1621 cgttatgctt ctcatgaacg agaacacaac ttattcatga attaaccatt ccggataaac
     1681 tatggaatca aattcaggca tcgaaaggaa ctggctggtt tccgttaata gattaaatat
     1741 ctattactct cccgagagta aaactatcaa atcaatgagt aaccgataca gcctcacaaa
     1801 ttctttcaga taattaaccc gtcactgcgg cacgttctct cgtaaggcgt gcctgcagtg
     1861 taatccagta tgtatccggc atagttgacg gtttaataga actcaattcc tctccctggt
     1921 caccaacaac gatatcttgt ataacacccc aggagatacg acattatgat gagatgcgat
     1981 atgctcctca gcaccaatca acgccaggac tgggccagtc ttgagatcag actcattccc
     2041 gggactgact tcagtcgaca tatatcaatg acgttggtgc aataaaagat agccgatgta
     2101 atacatctat tcaaatttgt gatacatgtc aaaaaactag ttgaccaaac tcgttattta
     2161 tataagggca cttacgaagt gcgctctttt ttaaagcgag gaagtaccaa tgaaagagaa
     2221 taaagtacaa caaatcagtc ataaactaat taatatcgtt gtttttgtcg caattgtaga
     2281 atacgcctat ttatttctcc atttctatta ataacggcaa taaactgttc acttcagtga
     2341 tatttaaaat atgcattctc tccctttttg taagtaatta ttatatccgt gggagaggaa
     2401 tacacgctgt caggtaatca atcatactgc gataaatcat cggccagtaa agtggagata
     2461 acctccattg tcgaaaaatc catactctca gcgaaaccat catcaatcac tcatccaggc
     2521 gtttatggga gcgtcgccaa cggctgttaa caatgccaga cttccccgtt gcggaaattc
     2581 cacataccac taatggtcac agggattggg tgttgaaatg atccggatga gcatgtatct
     2641 ttatggttat gctataacat aacaggtaaa aatgatgaag cccaatatcc atcctgagta
     2701 tcgtactgtg gtgttccacg acaccagtgt tgatgagtac tttaaaatcg gctcgactat
     2761 caaaacagac cgtgagattg agctggatgg cgtaacgtat ccgtacgtga caattgatgt
     2821 ctcttctaaa tcgcacccgt tctatacagg gaagctgaga acagtggcat cagaaggaaa
     2881 tgttgcacga ttcacccaac gttttggtcg ttttgttagc acgaaaaagg ggtcgtgatg
     2941 aaagttctta actctctgcg taccgcaaaa gaacgccatc cagactgtca gattgtgaag
     3001 cgaaaaggac ggctatatgt gatttgtaaa tctaatccac gttttaaggc cgttcagggg
     3061 cgtaagaaaa aacgttgatt caaaattcga cggattaacg atatttgtct gattaataat
     3121 cagatcggat taatgttggt gtgtttataa caccaacatt aattttcctg gggatatatt
     3181 cttcctgttc atttgaggcc aactgcctga cgtttctcta cgaatattcc attatcttaa
     3241 tgttgacttg ttgaccagct tcgcccctgt atgctggcat caactctctt ttagactgaa
     3301 cacgccactc agtctcctcc ctttgcggcg cagcctgcat tttcactcaa actgttaaga
     3361 tgataaatgt ggtaaatctg ttggtactaa cataaaaacg tttacgccac aggaacagtc
     3421 tgatccaccg gtaaccccgt cgccgacgtt cgagtgccag ttagagtaac gcgcacagat
     3481 aactgcactc agtgccctga caaagaggcc atcgttcctg tgacagctgg cagccttcgt
     3541 ttaacttcac ttaatctggc tcttgagggc ttaccgaaca gatgacgtac atacgcccgt
     3601 tcaattttcc atcacttatt gtaatgaaca cctataacca ttttgtgcgg catgttaatc
     3661 cattaaaatt tcttactgat tggcaaatca tcttcaatga cagctcatta tagttttata
     3721 ttctatccct tacccttaaa acttgttttt ttactagtcc atcacacagc gcattaagac
     3781 tattcctaac acttcagggc aaagttcctg accaatataa aatgcaagta agaattgaac
     3841 gttatattgc caataacctt atgaaaccaa atgtcttttt cttcttatca aaaaagcaat
     3901 attttcagtt tttctaaata ttgacttaac cattgaattc cttttccgtt cacatattga
     3961 cactcatcga aaaaaaaaca taaatttaag cccaatcgaa aataattaaa cttaatctcg
     4021 tttaaccttt attgatatgt actacgtatc ttatttactt ccggtttact aaggaaactg
     4081 aatgcacctg taaaaattac aggtttggaa agtagtgaca tggcaaagtg attacagtag
     4141 ggactatgag gttaaaaacc atatggaatg tcaaaaccgt tctgataaat acatctggtc
     4201 tccccatgac gcctacttct ataaaggact atctgaactg attgtggata tcgacagatt
     4261 aatttatctg tcgttggaga aaattagaaa agatttcgtg tttatcaatc tcaatacgga
     4321 ttctttatct gaatttataa accgtgataa tgaatggtta tccgcggtaa aggggaaaca
     4381 ggtcgtattg attgcggcca gaaagtcaga agccttagct aattattggt actacaacag
     4441 caatattagg ggcgtggtat acgctggact gagtcgtgat attagaaaag aactggccta
     4501 tgtgattaat ggcaggttcc tgagaaaaga tattaagaaa gataaaatca cggaccggga
     4561 aatggaaatt atccgcatga cggcccaggg aatgcaacct aaatcgattg ccagaattga
     4621 aaattgtagt gtgaagacag tgtataccca tcggcgtaat gctgaggcca agctgtactc
     4681 aaaaatatat aagttggttc agtaaactcc aggcaagtta gttttaaaaa atgactcact
     4741 gggacatcac gtcctcaatt caactcggga agaaatgcaa tgaaaaaaag gttctggcaa
     4801 tagctctggt aacggtgttt accggcatgg gtgtggcgca ggctgctgac gtaacagctc
     4861 aggctgtagc gacctggtcg gcaacagcca aaaaagacac caccagtaat ctggttgtga
     4921 cgtcactcgg tagcttggcg ttccagtatg ccgaaggcat taaaggtttt aactcacaga
     4981 aaggtctatt tgacgtggct atcgagggtg actcaacggc taccgccttt aaactgacct
     5041 cacgtcttat caccaacaca ttaactcagt tggatacctc aggttccaca ctgaatgtgg
     5101 gcgtggatta taacggcgcg gcagtcgaaa aaactggcga taccgtgatg atcgataccg
     5161 ccaacggcgt actgggcggc aaccttagcc cgctggctaa cggttacaat gccagcaatc
     5221 gtaccaccgc acaggatggt ttcaccttca ccatcatcag cggcaccacc aatggtacca
     5281 ccgcagtaac cgattacagc actctaccgg aaggcatctg gagcggcgac gttagcgtac
     5341 agttcgacgc gacctggacc agttaatctc tctgatgtac cagcaggggt agcccccctg
     5401 ctttattcct ggacggaatt gttatgaaaa agcaccttct gcctctcgct ctgctgtttt
     5461 ccggaatatc tccagcccag gcgctggatg tcggcgatat atcatcgttt atgaacagtg
     5521 acagcagcac gctgagcaaa acgatcaaaa acagtaccga cagtggtcgc cttatcaata
     5581 tccgtctcga acggctctct tcaccgcttg acgacgggca ggttatctca atggacaagc
     5641 cggatgagtt gctactcact cccgccagct tgctgctacc cgcccaagcc agcgaagtga
     5701 tccgcttctt ctataaggga cccgcagatg aaaaagagcg ctactaccgc attgtctggt
     5761 ttgatcaggc cctcagtgat gcgcagcgcg ataatgccaa ccgcagcgct gtggccactg
     5821 cttccgcccg catcggcacc attctggtcg tcgcccctcg tcaggcgaac taccactttc
     5881 agtacgccaa cggctccctg acaaatacag gaaatgcgac gctgcggatc ctcgcctacg
     5941 gcccctgcct gaaagccgcc aacggtaagg agtgtaaaga gaattactac ctgatgccgg
     6001 gcaagtcgcg tcgttttacc cgcgtggaca ctgcggataa caaaggacgg gttgcacttt
     6061 ggcagggtga taagttcatt cccgtgaaat agatagctgt gcagatggat aacgacaatg
     6121 cctttacgac ggttctcccc aggactgaaa gcccagtttg ccttcggcat ggtctttttg
     6181 ttcgttcagc ccgatgccag cgctgctgac ataagtgcgc agcaaatagg tggggtgatt
     6241 attccgcagg ccttcagtca ggcgcttcag gacggcatga gcgtcccgct ctatattcat
     6301 ctcgccggta gtcagggtcg ccaggacgat cagcgaatcg gcagcgcttt tatctggctg
     6361 gatgatggac agctacgcat ccggaaaata cagctggaag agagtgaaga taacgccagt
     6421 gtcagcgaac aaactcgaca gcagctgatg gctctggcga acgccccgtt caatgaggcc
     6481 cttaccatcc cctgactgac aacgcgcagt tggatctcag cttgcgccaa ctgctgctgc
     6541 agctggtggt caagcgcgaa gcgctgggca ccgtactacg ctcacgtagc gaagacatcg
     6601 ggcagtccag tgttaacacc ctcagcagta atctgagcta taacttgggc gtctataaca
     6661 accagttgcg taacggcggg agcaacacat ccagctatct gtcgctgaat aacgttactg
     6721 cgctgcgcga acatcatgtg gtgctcgacg gctcgctgta cgggatcggt agcggtcaac
     6781 aggacagtga attatataaa gcgatgtatg aacgcgattt tgccggtcac cgatttgccg
     6841 gtggaatgct cgacacctgg aacttgcagt cattagggcc gatgaccgcc atttcagcag
     6901 ggaagattta cggcctttcc tggggaaacc aggccagctc caccatcttc gacagcagcc
     6961 agtcagccac gccagtgatc gcctttttac cggcggcggg cgaagtacat ctcacccgtg
     7021 atgggcggct actaagcgtt cagaacttca ctatgggcaa tcatgaagtg gatacccggg
     7081 gtctaccgta cggtatttac gatgtggaag ttgaggtgat cgttaacggt cgcgtgatca
     7141 gcaaacgcac tcaacgggtc aataagctgt ttagccgggg cgcggcgtcg gtgcaccact
     7201 ggcgtggcag gtatggggcg gtagctttca tatggatcgc tggtcggaaa acgggaaaaa
     7261 gacgcgacca gctaaagaga gttggctagc aggtgcctcg acctccggct cattgagtac
     7321 gcttagctgg gcggcaacgg gatatggata cgataatcag gcggtgggtg aaacccgtct
     7381 gacgctgccg cttggggggg cgatcaacgt taacctgcaa aatatgctgg ccagtgacag
     7441 ctcatggagc agcatcggca gcatcagcgc tactctaccg ggaggcttta gttcgctgtg
     7501 ggttaaccag gaaaaaaccc gtattggcaa tcaattgcga cgtagcgatg ccgacaaccg
     7561 tgcaatcggc ggcacactca acctgaactc actgtggtcg aagctgggca cattcagcat
     7621 cagctacaat gatgaccgcc gttacaacag ccattattac acggcagatt actatcaaaa
     7681 tgtctacagc ggtacctttg gttcgcttgg cctgcgggcc ggtattcagc gctataacaa
     7741 cggcgacagc aacgccaata cagggaaata tatcgctctc gatctctcgc taccactggg
     7801 caactggttt agcgcaggga tgacccatca aaacggctac accatggcaa acctgtcagc
     7861 acgcaaacag tttgatgaag ggaccattcg cactgttggt gccaatctgt cacgtgccat
     7921 ctccggcgat accggtgatg acaaaaccct cagcggtggg gcgtatgcac agttcgacgc
     7981 tcgttacgcc agcggaacgc tgaacgtcaa tagcgcggcg gacggctacg tcaataccaa
     8041 cttgaccgcc aatggcagcg tcggctggca gggtaaaaac attgctgcca gcgggcggac
     8101 tgatggcaac gctggggtga tattcaacac cgggctggag gacgacggtc agatcagcgc
     8161 caaaatcaac gggcggattt tcccgcttaa cggcaagcgt aactatctcc cgctctctcc
     8221 ctatggaaga tatgaggtgg agttacagaa cagcaaaaac tcactcgaca gttacgatat
     8281 cgtcagcggc cgcaaaagtc gtctgactct ctatccaggc aatgtcgctg tcattgagcc
     8341 agaggtgaag cagatggtta ccgtctccgg tcgtatccgt gcggaagacg gcacactgct
     8401 ggctaacgca cggattaaca accatatcgg ccgaacccga accgatgaaa acggcgagtt
     8461 tgtcatggac gtggataaga aataccccac tatcgatttt cgctacagtg gcaataaaac
     8521 ctgcgaagtg gctctggaac tcaaccaggc gcgcggtgcc gtctgggtcg gtgatgtggt
     8581 ctgcagcggc ctctcatcgt gggcggcggt gacgcagaca ggagaagaga atgagagtta
     8641 acctactaat aacgatgata attttcgcgc taatctggcc agtaactgcg ctcagagcgg
     8701 cagtgagcaa aacaacctgg gcggatgcac cggcacgcga gtttgtgttt gtcgaaaaca
     8761 actcagacga caactttttc gtcactcctg gcggggcgct ggatccgcgc ctgaccggtg
     8821 ccaaccgctg gaccggttta aaatacactg gttcaggaac catctatcag caaagcctcg
     8881 gctacattga taacggttac aacaccggcc tttataccaa ctggaagttt gatatgtggc
     8941 tggaaaattc gccggtttca tctcctttaa ctggcttacg ctgcatcaac tggtacgcag
     9001 ggtgtaatat gaccaccagt cttatcctgc cgcaaaccac cgacgccagt ggattttatg
     9061 gcgcgacggt gaccagcggc ggcgcgaagt ggatgcacgg catgttgtca gacgcgtttt
     9121 accagtatat gcaacaaatg cccgtcggca gcagctttac aatgaccatc aatgcctgtc
     9181 agacctctgt gaactatgac gccagcagcg gcgcgcgttg taaggatcag gcctccggca
     9241 actggtatgt tcgcaatgtc acccatacga aagcagcaaa tcttcggtta ataaataccc
     9301 actcgctggc ggaggtattt atcaacagcg acggggtgcc gaccctgggc gaagggaacg
     9361 ccgactgccg gacacagacc atcggcagcc tttcaggatt aagttgtaag atggtcaact
     9421 ataccctgca aacaaacgga ctcagcaaca cctcaatcca tatattcccg gcgatcgcca
     9481 actcgtcgtt agcctcggcc gtcggggcgt acgatatgca gttcagtctg aatggcagtt
     9541 catggaaacc ggtgagcaat accgcctatt actacacctt caacgagatg aagagcgcag
     9601 actcgatcta tgttttcttc tcgagcaact tctttaagca gatggtgaac ctcg
//
LOCUS       DADLFA010000158         9592 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000158 DADLFA010000000
VERSION     DADLFA010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9592
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.160"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(159..983)
                     /locus_tag="JQH65_003244"
     CDS             complement(159..983)
                     /locus_tag="JQH65_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HAY6140563.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            1303..3333
                     /gene="malS"
                     /locus_tag="JQH65_003245"
     CDS             1303..3333
                     /gene="malS"
                     /locus_tag="JQH65_003245"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HAY6140564.1"
                     /translation="MKLASCFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLINKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            3511..4764
                     /gene="avtA"
                     /locus_tag="JQH65_003246"
     CDS             3511..4764
                     /gene="avtA"
                     /locus_tag="JQH65_003246"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HAY6140565.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNVFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(4915..5388)
                     /locus_tag="JQH65_003247"
     CDS             complement(4915..5388)
                     /locus_tag="JQH65_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAY6140566.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(5490..6338)
                     /gene="yiaJ"
                     /locus_tag="JQH65_003248"
     CDS             complement(5490..6338)
                     /gene="yiaJ"
                     /locus_tag="JQH65_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HAY6140567.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            6539..7537
                     /gene="yiaK"
                     /locus_tag="JQH65_003249"
     CDS             6539..7537
                     /gene="yiaK"
                     /locus_tag="JQH65_003249"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HAY6140568.1"
                     /translation="MKVTFEQLKAAFNRALISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            7549..8017
                     /locus_tag="JQH65_003250"
                     /pseudo
     CDS             7549..8017
                     /locus_tag="JQH65_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF386 domain-containing protein"
     gene            8135..8608
                     /gene="yiaM"
                     /locus_tag="JQH65_003251"
     CDS             8135..8608
                     /gene="yiaM"
                     /locus_tag="JQH65_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HAY6140569.1"
                     /translation="MKKILEVILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            8611..>9592
                     /locus_tag="JQH65_003252"
     CDS             8611..>9592
                     /locus_tag="JQH65_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAY6140570.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPDMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSTWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVG"
BASE COUNT         2325 a         2459 c         2387 g         2421 t
ORIGIN      
        1 gacacaacgc caaaagagta tcgcgatgta aatcgcgagg tcatgttgta gcgatgatga
       61 gaattgtcgg cgtcacatca gggaactcag cacagccacc tgatgcgagc aaaagcctta
      121 tccgaccgac aaatcagata aggctcaagg aaatgcaatt acatatgcgc cgcgattaac
      181 cgttggttat cctggtacat tgcgaacagg tagttattat aactcttccc tttggtcgag
      241 tagcccttca gcttgtgaat catcgctgtg gcagtcactt cctgatccgc tttacgcagc
      301 tgcgcacgcg atttacggaa cgaagagtaa gccgggtgcg tattcaggtt agtgacatag
      361 gcgctcaccg attctttgac agaactaaac tgtgagtacc ctttcacttt acccggcgca
      421 ttggtacaac gtcctttcat gcatttcatg ccgaacagat tgttattgtt gcgcgccagc
      481 ttcgacgttc cccaaccgct ttctgctgca gccatcgtcg ccaccatact ggtagggata
      541 atgtctacgc gttcaagcaa ggtattccac gggatttttc gcgtattacc agaccacttc
      601 accttgtagc gtttggcgat gtctttcaga cgcgcacgct cagcaggtga ccattggccc
      661 tgatactgtt ttgaaatcag ccagttacgt tctgcagtaa tggctgcatt ttggctggta
      721 atgtaaggca ttacggtccg gagaaacgcc tttttccttg gtgttccgga agggtatttt
      781 cgcaaatcag gaagtgaact actctttgca ctattgcgag aatactcttg tttactgctt
      841 acctgtttat tactagcttt agttaagtgg gacttttgac tcgctgttgt tgtgtgcgtc
      901 ttcgctaaca cctcactcga aaacaccaga gtgagtaaca taagaatcat cgccccatat
      961 cgtcgtatgg gagtcaaaat catcaggtct cctggtcgga tttaatcatt ccaacacctt
     1021 atatttttca caaatttgag agttgaatct caaatcatat caaaaatagc tgtcaagagc
     1081 accccaagga atagtccaaa tctgaaacta tgtcacgagt taacgattca gattggcgct
     1141 aaatcgcaga aaatgtgggg tttatcgcaa aattcagccg ttttttgcgc gcgatcgctc
     1201 acccttgctt ctcatcctgt ggacttaccg ctcagggatg agttttgtct ggcttatcgc
     1261 tggcaaactg tctgaaatcg cagcaataag gactcatcca ccatgaaact cgcctcctgt
     1321 tttctgacac tccttcctgg cttcgccgtt gccgccagct ggacttctcc ggggttccct
     1381 gcctttagcg aacagggaac gggaacattt gtcagccacg cgcagttgcc caaaggtacg
     1441 cgtccactca cgctaaattt tgaccaacag tgctggcagc ctgcagatgc gataaaactc
     1501 aatcagatgc tttccctgca accttgtagc aacacgccgc ctcaatggcg attgttcagg
     1561 gacggcaaat atacgctgca aatagacacc cgctccggta cgccaacatt gatgatttcc
     1621 atccagaacg ccgccgaacc ggtagcaaac ctggtccgtg aatgcccgaa atgggatgga
     1681 ttaccgctca cgctggatgt cagcgccact ttcccggaag gagccgccgt acgggattat
     1741 tacagccagc aaattgcgat agtgaagaac ggtcaaataa cgttacaacc cgctgctacc
     1801 agcaacggtt tactcctgct ggaacgggca gaaactgaca catccgcccc tttcgactgg
     1861 cataacgcca cggtttactt tgtgctgaca gatcgtttcg aaaacggcga tcccagtaat
     1921 gaccagagtt acggacgtca taaagacggt atggcggaaa ttggcacttt tcacggcggc
     1981 gatttacgcg gcctgatcaa caaactggat tacctccagc agttgggcgt taatgcttta
     2041 tggataagcg ccccatttga gcaaattcac ggctgggtcg gcggcggtac aaaaggcgat
     2101 ttcccgcatt atgcctacca cggttattac acacaggact ggacgaatct tgatgccaat
     2161 atgggcaacg aagccgatct acggacgctg gttgatagcg cacatcagcg cggtattcgt
     2221 attctctttg atgtcgtgat gaaccacacc ggctatgcca cgctggcgga tatgcaggag
     2281 tatcagtttg gcgcgttata tctttctggt gacgaagtga aaaaaacgct gggtgaacgc
     2341 tggagcgact ggaaacctgc cgccgggcaa acctggcata gctttaacga ttacattaat
     2401 ttcagcgaca aaacaggctg ggataaatgg tggggaaaaa actggatcag aacggatatc
     2461 ggcgattacg acaatcctgg attcgacgat ctcactatgt cgctggcctt tttgccggat
     2521 atcaaaaccg aatcaactac cgcttctggt ctgccggtgt tctataaaaa caaaacggat
     2581 actcacgcta aagtcatcga aggctttaca cctcgcgatt acttaaccca ctggttaagt
     2641 cagtgggtcc gcgactatgg gattgatggt tttcgggtcg ataccgccaa acatgttgag
     2701 ttgcccgcct ggcagcaact gaaaaccgaa gccagctccg cgcttcgcga atggaaaaaa
     2761 actaaccccg acaaagcatt agatgacaaa cctttctgga tgaccggtga agcctggggc
     2821 cacggcgtga tgcaaagtga ctactatcgc cacggcttcg atgcgatgat caatttcgat
     2881 tatcaggagc aggcggcgaa agcagtcgat tgtctggcgc agatggatac gacctggcag
     2941 caaatggcgg agaaattgca gggtttcaac gtgttgagct acctctcgtc gcatgatacc
     3001 cgtctgttcc gtgaaggggg cgacaaagca gcagagttat tactattagc gccaggcgcg
     3061 gtacaaatct tttatggcga tgaatcctcg cgtccgttcg gtcctacagg ttctgatccg
     3121 ctgcaaggta cacgttcgga tatgaactgg caggatgtta gcggtaaatc tgccgccaac
     3181 gtcgcgcact ggcagaaaat cagccagttc cgcgcccgcc atcccgcaat tggcgcgggc
     3241 aaacaaacga cactttcgct gaagcagggc tacggctttg ttcgtgagca tggcgacgat
     3301 aaagtgctgg tcatctgggc tgggcaacag tgacttttcc ggcttcccgt tcatcagtac
     3361 ctcgggaagc cgccaaccag gataaaatgt cagccctaat cagcgttgca ggataaagca
     3421 ccgctcactc ttcaacagac cgatttgcac cccagcaaat gtagcgttat tgttaccttc
     3481 cttgctacag agttcgacag atatcccgct atgacattct ccctttttgg tgacaaattt
     3541 acccgccact ccggcattac gctgctgatg gaagatctga acgacggttt acgcacgcct
     3601 ggcgcgatta tgctcggcgg cggtaatccg gcgcagatcc cggaaatgca ggactacttc
     3661 cagacgctac tgaccgacat gctggaaagt ggcaaagcga ctgatgcact gtgtaactac
     3721 gacggtccac aggggaaaac ggagctactc acactgcttg ccggaatgct gcgcgagaag
     3781 ttgggttggg atatcgaacc acagaatatt gcactaacaa acggcagcca gagcgcgttt
     3841 ttctacttat ttaacctgtt tgccggacgc cgtgccgatg gtcgggtcaa aaaagtgctg
     3901 ttcccgctgg caccggaata cattggctac gctgacgccg gactggaaga agatctgttt
     3961 gtctctgcgc gtccgaatat tgaactgctg ccggaaggcc agtttaaata ccacgtcgat
     4021 tttgagcatc tgcatattgg cgaagaaacc gggatgattt gcgtctcccg gccgacgaat
     4081 ccaacaggca atgtgattac tgacgaagag ttgctgaagc ttgacgcgct ggcgaatcaa
     4141 cacggcattc cgctggtgat tgataacgct tatggcgtcc cgttcccggg tatcatcttc
     4201 agtgaagcgc gcccgctatg gaatccgaat atcgtgctgt gcatgagtct ttccaagctg
     4261 gggctgcctg gctcccgctg cggcattatc atcgccaatg aaaaaatcat cactgccatc
     4321 accaatatga acggcattat cagcctggca cctggcggta ttggtccggc gatgatgtgt
     4381 gaaatgatta agcgtaacga tctgctgcgc ctgtctgaaa cagtcatcaa accgttttac
     4441 taccagcgtg ttcaggaaac tatcgccatc attcgccgct atttaccgga aaatcgctgc
     4501 ctgattcata aaccggaagg agccattttc ctctggctat ggtttaagga tttgcccatt
     4561 acgaccgagc agctctatca gcgcctgaaa gcacgcggcg tgctgatggt gccggggcac
     4621 aacgtcttcc cgggactgga taaaccgtgg ccgcatacgc atcaatgtat gcgcatgaac
     4681 tacgtaccag agccggagaa aattgaggcg ggggtgaaga ttctggcgga agagatagaa
     4741 agagcctggg ctgaaagtca ctaaaaattg cctgatgcgc tacgcttatc aggcctacat
     4801 gatttctgca atatattgaa tttggaagaa tttgtaggcc ggataaggcg tttacgccgc
     4861 atctggcata aacaaagcgc actattccgg cgttgagaaa cgccggaaaa cgtttcaaac
     4921 aggctgctgc cgtagccgat gccgctgcac tttctcgaca tccatgcact gcaacgcctt
     4981 tgtcgggcag gcttcaacac aggccggccc cgtctcccga tgccagcaca gatcgcactt
     5041 aatcgccctc gcctttttgc gcgaagaaac gacctccatc gcaccaaacg ggcaagccag
     5101 catacagctt ttacagccaa tgcaacgtgt ttgttcaacg aaaatatgcc catgttcgcg
     5161 gcttatcgcg tcaacagggc agacattcgc gcacggtgca tcttcacact gatgacaggc
     5221 tactgccgtg gtccagctgt ggtctttaat gacacgaata cgggaaataa actcgtctgg
     5281 tgacaacgca gcgcaatcct gattctcctg atgcgacact gcgcaagcca cttcacaggt
     5341 acggcaaccg atacatttcg tcgcatccgc aataataaac cggttcatga tcactccttt
     5401 gcactaaaac agcagagtgc cagagagcgc gtccctactg ctttgatcca acgaaggaaa
     5461 tgttaaagtt ttggcacttg tccaaagcgt tatgtgatcg cgcccaggtc atccctgaca
     5521 gtaaatccca gttcattaga aatagcctgc gcggtttcac gcagtggttt caggagattt
     5581 ttctctccca cctgtttcag acgtgatgtc gaaagcgaaa tcgacacggc gtacggcacc
     5641 cgcccatgaa tatcaaacac cggaacagca atacaggaga cgccgagttc gttttcttct
     5701 ctgtccatcg ccgctccgct ttcacgaatg tgcgccagtt cgtcgaacat cgcgggcagc
     5761 tcggtaatgg tattgcgggt taacggctgg atctcatgct gatggttttc ccagtatgac
     5821 ttcacgtagt ccgggtgacc aaacgccatg tagatcttgc ccattgcgga acagtagagc
     5881 ggcatatgct ggccaatata ggcacgggtt cgcagcatcc cggttgtggg ttccagctta
     5941 taaatcaaaa tagcgtgatc gtcttcgcgg ctggagaagt taatggtttc accagtggcg
     6001 atgttcagtg cctcaagatg cggagcggcg atatgaatga tattcagcga agacagcgcc
     6061 ttctgcccga cggcaataaa tttggtagtc aggcggtaac tccctgcggc gggcgcggtg
     6121 gtcacatagc cgcaggactg taatccctgc aataagcgat ggacggtact cttatttaaa
     6181 ccagccagct ccgaaagatg cgccaacgga caaccgtttg gatagttgct caaaatctca
     6241 atcagcatca acccgcgaaa cagactctgg cttccggctg gacgctcttt ttcctgcgcc
     6301 atctcgttct ctttttttcc catcacttct ttccccattt tgtcgcgtcc tgatggtagc
     6361 gcaaagtgtg ccgtagttca cgatctcgac agataattta taaccacttg atttttatgt
     6421 cttttgaaat tcatcaatca gattgctttg ttaaaaagtg atcgatatat ttgaaatcaa
     6481 gtttcgcata ttgaaatttt aagccaaaaa agcgatcaaa aaaacaagga agcctgggat
     6541 gaaagtgaca tttgagcagt taaaagcagc ctttaatcgg gccttaattt cacgcggcgt
     6601 tgacagcgaa acggctgacg cctgtgcaga gatgttcgcc cgcaccaccg aatccggcgt
     6661 ttattctcac ggcgttaatc gtttccctcg tttcattcaa caactggaaa acggcgatat
     6721 cattcctgat gcccaaccca aacgtataac cagcctcggc gcaattgaac agtgggatgc
     6781 ccagcgttcg atcggcaacc tgacggcgaa aaagatgatg gatcgcgcca ttgaactggc
     6841 tgccgatcac ggtattggtc tggtggcact tcgtaatgcc aaccactgga tgcgcggcgg
     6901 cagctacggc tggcaggcgg cggaaaaagg ctatattggc atttgctgga ccaactccat
     6961 cgccgtaatg ccgccgtggg gcgcaaaaga gtgtcgcatt ggcactaacc cgctgatcgt
     7021 cgccattcct tccacgccga tcaccatggt cgatatgtcg atgtcgatgt tctcttacgg
     7081 catgttagaa gttaaccgcc tggcgggccg tcagctcccg gtcgatggtg gctttgatga
     7141 tgagggcaat ttgaccaaag aacctggcgt tatcgagaag aatcgccgca ttttgccgat
     7201 gggctactgg aaaggttctg gcatgtcgat tgtgctggat atgatcgcta ctctcctttc
     7261 cgacggcgca tcggttgccg aagtcaccca ggacaacagc gacgaatacg gcatttcaca
     7321 aatttttatt gccattgaag tggacaagct tatcgacggt cccacccgcg atgccaagct
     7381 gcaacgcatc atggattacg ttactaccgc tgaacgcgct gacgaaaacc aggccatccg
     7441 cttacccggc cacgaattta ctaccctgct ggccgaaaac cgccgtaacg gcatcactgt
     7501 tgatgacagc gtgtgggcca aaatccaggc gttatgagga gataagtcat gatttttgga
     7561 catatcgcgc agcccaatcc gtgccgtttg cccgccgcca ttgaaaaggc gctcgatttt
     7621 ctgcgcgcta ccaacttcaa cgtcctggag ccgggcgttg tcgaaatcga cggcaagaat
     7681 atttatgcgc aaattatcga tttaaccaca cgcgaagcgg tggaaaaccg tccggaagtg
     7741 catcgtcggt atatcgatat tcagtttctg gcgtggggtc aagagaaaat cggcattgct
     7801 attgatacag gaaataataa agtcagcgaa tcactattag agcagcgcga tattattttt
     7861 tatcatgtca gtgaacatga atcatttata gaaatgatac cgggcagcta cgctattttc
     7921 ttcccgcagg atgttcaccg accaggttgt attctgcaaa caggcctctg agatacgaaa
     7981 aattgtggtt aaagtcgcat taacagcgct gaattaatca aataatcgtc tacgccagaa
     8041 cgactgggta atgttattgc tctaactata ttaataattt aggttgctgc acatgcaact
     8101 tgaattatga cagacatagc ctcaaggaat agctatgaaa aaaatacttg aagtaatact
     8161 ggcgattaat ctcgccgtac tttcctgtat tgtgtttata aacattattt taagatatgg
     8221 ttttcagaca agcattttat ctgtagatga attgtcacgt tatttatttg tctggttaac
     8281 atttattggc gcgattgtgg cttttatgga taacgcccac gttcaggtca cctttctggt
     8341 ggagaaactc tcccccgcat ggcaacgacg agtcgctctg gttacccatt ccttaatctt
     8401 atttatttgt ggcgcactgg cctggggcgc aacactaaaa acaattcagg actggagcga
     8461 ttattcaccg atcctcggtt tgcccatcgg cctgatgtat gccgcttgtc tgcccaccag
     8521 cctcgttatc gcattctttg aactgcgtca tttatatcaa ctcatcacgc gtagcaattc
     8581 tctaacgtcg ccaccgcaag gagcttaatc atggctgtgc tgatttttct gggctgtctg
     8641 ttgggtggta ttgctatcgg tttgcctatt gcctgggcac tgttgttgtg cggggcggcg
     8701 ttaatgttct ggctggacat gtttgatgtc cagatcatgg cacaaacgct ggtgaacgga
     8761 gccgatagct tctccctgct ggcgattccg ttctttgtgc tggcgggtga aatcatgaat
     8821 gcgggcggct tgtcaaaacg cattgttgac ctgccgatga aactggtggg gcataaacca
     8881 ggcgggctgg gctacgtcgg cgtgctggca gctatgatta tggcgagcct ttctggttct
     8941 gccgttgcgg ataccgccgc cgttgccgct ctactggtgc cgatgatgcg cagcgccaac
     9001 tacccggtca accgggcggc ggggctgatt gcctctggcg gcattatcgc gccaattatt
     9061 cccccttcca ttccgtttat tatcttcggc gtttccagcg gattatccat cagtaagctg
     9121 tttatggcgg gcattgcccc tgacatgatg atgggcgcaa cgctgatgct tacctggtgg
     9181 tggcaggcca gtcgccttaa tttaccccgc cagcaaaaag caacgatgca ggaaatctgg
     9241 cactcttttg tctccggtat ctgggctctg tttcttccag tcatcattat tggcgggttt
     9301 cgctccggtc tgtttacccc taccgaagcc ggggccgttg ctgctttcta tgcgctgttt
     9361 gtcgccacag ttatttaccg tgaaatgacc ttcgccacac tttggcatgt actcattggc
     9421 gcggcgaaaa ccacctcagt ggttatgttt ctggtggcct cggcacaagt ttccacctgg
     9481 ctgattacca ttgctgaact gccgatgatg gtttctgatt tactgcaacc gttggtcgat
     9541 tcaccgcgtc tgctgtttat cgtcattatg gtggctatcc tgattgtcgg ca
//
LOCUS       DADLFA010000159         9589 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000159 DADLFA010000000
VERSION     DADLFA010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9589
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.161"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(10..1230)
                     /gene="fabB"
                     /locus_tag="JQH65_003253"
     CDS             complement(10..1230)
                     /gene="fabB"
                     /locus_tag="JQH65_003253"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HAY6140571.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            1389..3395
                     /gene="mnmC"
                     /locus_tag="JQH65_003254"
     CDS             1389..3395
                     /gene="mnmC"
                     /locus_tag="JQH65_003254"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HAY6140572.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQQDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAC
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNLLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(3516..3794)
                     /locus_tag="JQH65_003255"
     CDS             complement(3516..3794)
                     /locus_tag="JQH65_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HAY6140573.1"
                     /translation="MIAEFESRILALIDGMIDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(3828..4376)
                     /locus_tag="JQH65_003256"
     CDS             complement(3828..4376)
                     /locus_tag="JQH65_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HAY6140574.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(4376..5185)
                     /locus_tag="JQH65_003257"
     CDS             complement(4376..5185)
                     /locus_tag="JQH65_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HAY6140575.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFAGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(5185..6009)
                     /gene="mepA"
                     /locus_tag="JQH65_003258"
     CDS             complement(5185..6009)
                     /gene="mepA"
                     /locus_tag="JQH65_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HAY6140576.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFAPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(6013..7098)
                     /gene="aroC"
                     /locus_tag="JQH65_003259"
     CDS             complement(6013..7098)
                     /gene="aroC"
                     /locus_tag="JQH65_003259"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAY6140577.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGILLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(7133..8065)
                     /gene="prmB"
                     /locus_tag="JQH65_003260"
     CDS             complement(7133..8065)
                     /gene="prmB"
                     /locus_tag="JQH65_003260"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAY6140578.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPDAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            8231..8782
                     /gene="smrB"
                     /locus_tag="JQH65_003261"
     CDS             8231..8782
                     /gene="smrB"
                     /locus_tag="JQH65_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HAY6140579.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(8855..9568)
                     /locus_tag="JQH65_003262"
     CDS             complement(8855..9568)
                     /locus_tag="JQH65_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005007603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HAY6140580.1"
                     /translation="MATPDSVNYAIFKATFMPNSQPDLVSWTGDSSTQPSMSKISDSR
                     VSMSACPGLEQYDSQTKTGWTCNELKMFVYYDGNLHGCPWIVSSFVKSRDPFAKTYDD
                     DFPDYIGPTKVSSSCPAVPLAPYDVSWNENYVVHNKVVRLQSTGGVIEQTLPTFLMEN
                     GKLCNGNNFDERGVYCRFIAQQMTFSTSGCDNAKVTVTPEPQPITSRQLHDMKLRVDT
                     TSRQPIDSTCRFTYILNMY"
BASE COUNT         2291 a         2514 c         2564 g         2220 t
ORIGIN      
        1 tactgcgaat taatctttca gcttgcgcat taccagcgtg gcgttggtgc cgccgaagcc
       61 gaagctgtta gacataacgg tggtcagttc gcgatcggtc gtttcggtca cgatgttcag
      121 acctgccgcc tgctcgtcca gctcttcaat gttgatgctc ggggcgataa agccgtgttc
      181 cagcatcagc agagagtaga tagcttcctg tacgccagca gcgcccagag agtgaccggt
      241 catggctttg gttgcagaaa tcgccgggct cttatcgccg aacacttcac ggatagctgc
      301 cagttctttc acgtcgccaa ccggagtcga agtaccatga gagttcaggt aatcgattgg
      361 ggtatcaacg ccgtgcatcg ccatcttcat gcagcgtact gcgccttcgc cagacggagc
      421 aaccatgtct gcaccatcag aggttgcgcc gtagccaacg atttcagcat agatgtgagc
      481 accacgcgcc agcgcgtgtt ccagctcttc aaccactacc ataccgccgc cgccagcgat
      541 aacgaaaccg tcacggtgag cgtcgtaagt acgggaggct ttttccgggg tgtcgttgta
      601 tttagtagac agcgcaccca tcgcgtcgaa ttcgcaggcc atttcccagc acagctcttc
      661 gccgccgcca gcaaacacga tgtcctgttt gcccagttgg atctgctcta ctgcgttacc
      721 gatacagtgt gcggaagtcg cacacgcgga gctgatggag tagttaacgc catgaatttt
      781 aaacggcgtg gcgaggcagg cagaaacgcc ggatgccatc gctttggtga ccacatacgg
      841 gccaaccgct ttcaggccgc gcgggccgcg cattgcgtca gcgccgaata cctggaaacg
      901 cggggaaccg ccgccagaac ctgcaatcag gccaacgcgc gggttattct ggtaagcttc
      961 cggagaaagg cccgcatctg cgattgcctg ctccatagaa aggaatgcat aaatggatgc
     1021 gtcgctcata aagcgcacaa ctttgcggtc aatgaggcca gtggtatcca gttttacgtt
     1081 gccccagacg tggctacgca tgccggaatc cttaagctcc tgagagaaag tgatccctga
     1141 acgtccttca cgcagagatg ccaggacttc ctgctggtta ttaccgatgc tggaaacaat
     1201 gcccaggcca gtaatcactg cacgtttcat tcaatacctc tgtaagtcgc acatagagta
     1261 agtttcgaat gcacaatagc gtacacttgt acgccgaaca agtccgatca gccatttaat
     1321 agagaaattt gcgcagcctt acacacatcg ctaagatcga gccaccgcct gtaagacgag
     1381 taacttacgt gaaacactac tccatacaac ctgccaacct cgaatttaat gctgagggta
     1441 cacctgtttc ccgagatttt gacgatgtct atttttccaa cgataacggg ctggaagaga
     1501 cgcgttatgt ttttctggga ggcaaccaat tagaggtacg ctttcctgag catccacatc
     1561 ctctgtttgt ggtagcagag agcggcttcg gcaccggatt aaacttcctg acgctatggc
     1621 aggcatttga tcagtttcgc gaagcgcatc cgcaagcgca attacaacgc ttacatttca
     1681 ttagttttga gaaatttccc ctcacccgtg cggatttagc cttagcgcat caacactggc
     1741 cggaactggc tccgtgggca gaacaacttc aggcgcagtg gccaatgccc ttgcccggtt
     1801 gccatcgttt attgctcgat gaaggccgcg tgacgctgga tttatggttt ggcgatatta
     1861 acgaactgac cagccaacag gacgattcgc taaatcaaaa agtagatgcc tggtttctgg
     1921 acggctttgc gccagcgaaa aacccggata tgtggacgca aaatctgttt aacgccatgg
     1981 caaggttggc gcgtccgggc ggcacgctgg cgacatttac gtctgccggt tttgtccgcc
     2041 gcggtttgca ggacgccgga ttcacgatgc aaaaacgtaa gggctttggg cgcaaacggg
     2101 aaatgctttg cggggtgatg gaacagacat taccgctccc ctgctccgcg ccgtggttta
     2161 accgcacggg cagcagcaaa cgggaagcgg cgattatcgg cggtggtatt gcctgcgcgt
     2221 tgttgtcgct ggcgctatta cggcgcggct ggcaggtaac gctttattgc gcggatgagg
     2281 cccccgcact gggtgcttcc ggcaatcgcc agggggcgct gtatccgtta ttaagcaaac
     2341 acgatgaggc gctaaaccgc tttttctcta atgcgtttac ttttgctcgt cggttttacg
     2401 accaattacc cgttaaattt gatcatgact ggtgcggcgt cacgcagtta ggctgggatg
     2461 agaaaagcca gcataaaatc gcacagatgt tgtcaatgga tttacccgca gaactggctg
     2521 tagccgttga ggcaaatgcg gttgaacaaa ttacgggcgt tgcgacaaat tgcagcggca
     2581 ttacttatcc gcaaggtggt tggctgtgcc cagcagaact gacccgtaat ctgctggaac
     2641 tggcgcaaca gcagggtttg cagatttatt atcaatatca gttacagaat ttatcccgta
     2701 aggatgactg ttggttgttg aattttgcag gagatcagca agcaacacac agcgtagtgg
     2761 tactggcgaa cgggcatcaa atcagccgat tcagccaaac gtcgactctc ccggtgtatt
     2821 cggttgccgg gcaggtcagc catattccga caacgccgga attggcagag ctgaagcagg
     2881 tgctgtgcta tgacggttat ctcacgccac aaaatccggc gaatcaacat cattgtattg
     2941 gtgccagtta tcatcgcggc agcgaagata cggcgtacag tgaggacgat cagcagcaga
     3001 atcgccagcg gttgattgat tgtttcccgc aggcacagtg ggcaaaagag gttgatgtca
     3061 gtgataaaga ggcgcgctgc ggtgtgcgtt gtgccacccg cgatcatctg ccaatggtag
     3121 gcaatgttcc cgattatgag gcaacactcg tggaatatgc gtcgttggcg gagcagaaag
     3181 atgaggcggt aagcgcgccg gtttttgacg atctctttat gtttgcggct ttaggttctc
     3241 gcggtttgtg ttctgccccg ctgtgtgccg agattctggc ggcgcagatg agcgacgaac
     3301 cgattccgat ggatgccagt acgctggcgg cgttaaaccc gaatcggtta tgggtgcgga
     3361 aattgttgaa gggtaaagcg gttaaggcgg ggtaatctgc tctcgcagtg tttgccggat
     3421 gcggcgtgaa cgccttatcc gacctacgtg tgacgtgtag gcctgataag acgcgataag
     3481 cgtcgcatca ggcatggtgc tccagacgcc gcaacttact gctgtgacgc ctgctgaaac
     3541 aggttttccc acatatcggt caccagcgcc tggtcacgcg gcgacaattc accggcacca
     3601 atggcttttt ccagactctg gctaacggtc gtatgcaccg cctgagcgga gtggtcatca
     3661 ccactttcca gttctgcgat ggctaatgtc aggtggccac gcaaataccc actggcaaac
     3721 aactcatcat cactggcatg gtcaatcatg ccgtcgatta atgccagaat gcgtgattca
     3781 aactccgcga tcatcttctt tcctcattgt aaaacgtggc accggcttca gttgagcgct
     3841 tccggccacg ggaactgttc cgccgtaagt tccggcgtgt gataataatt ttgtagtgct
     3901 ttaatgaagc gtgccggacg ctcgggaatg ccatttgtca gataatccat cacctgcgca
     3961 tgtacccgcc gttgaaacac cacacgatcc ggttcgaagt cgccttccag attgtcgcaa
     4021 ctgacgttaa acggatatcc cgccgccaca cagaacaacc agtcgagtgc ctgcggcttc
     4081 acttcaacat cttcaaactg gctttgcgtt tgggcatcac gcccatccgg gcaataccag
     4141 tagccgaaat caaccagctc acggcgcgct tttccggcaa tacaccagtg cgaaatctca
     4201 tgaattgcgc tggcataaaa gccgtgggca aagacaattc ggttatacgg tacttccgca
     4261 tcagcaggaa gatagatcgg ttcgtcgtcg cctttaatca gacgggtatt aaattcatcg
     4321 gcaaagcagc tattaaaaat ttcaatcaac tgctcatagt ggtgtgtact gttcattagt
     4381 tcatccccaa ccagtggagg atctcctgtc cgtggctatc gtaaagtaat ttggcactca
     4441 tcaccgccga gacgataacg atcatcgggc ggatcagttt ttgtcctttg ctcagcacca
     4501 gtcttgaacc catgcgcgcg ccgaggaact gccccaccag catgacaaag cccgtcgccc
     4561 aaatcacttt gccgccgaga ataaacagca gcagaccgcc aatgtttgac gttgcgttga
     4621 gtaatttggc gtgagccgtg gctttggcga ggttgaatcc gcacagcgta acgaaggcca
     4681 gcgcgtaaaa cgatccggca gccgggccaa agaatccgtc ataaaaaccg acgcagccac
     4741 cggcaattaa cgcgaagggt aaaccgtaca tccggcgctg gcgatcttct tcacccagct
     4801 ttggcatcag caagaaatag aggccgatac aaattaccag aatgggcaaa atctgccgta
     4861 agacatcagc ctgaacgtat tgcaccagca gtgcgccgct cattgagccg gcaaaggtca
     4921 tggcgatatt gagtttctga tcgcttaaac taaccacttt gcggcgaata aagtagatag
     4981 tggcggaaat agagccgccg caggcttgca gtttattggt tgccagcgcg ttagcgggag
     5041 acattcccgc agccatcaat gccggaatgg tgagtaaccc accgccaccg gcaatcgagt
     5101 cgataaatcc cgccagcatg gcgacaaaaa agagaactcc cagcaacagc ggggaaacca
     5161 taaacaggct attaaacgtt tccattagat cacgtgctca tccagtagcg cctggcagga
     5221 aggcggcaac ggaggcggtg tcttcttctc aggctttgtt gttcctggtt ttggaggtgc
     5281 aaaccagctt tgcagttctg ccccgcaacc atcgcctggt ggcggtaaag gttgatcttc
     5341 acactccaga ctatcggcag ggcaacgtaa tcgtacatgc atatgcgcgc gatgctggaa
     5401 ccagggtcgc actttgcgca accagtcgcg atcggtgccc gcatcaaggc aaagttgttg
     5461 tttaatcgcc ggattaacaa aaatgcgcgt gacgtctttg tcctgggcgg cgagtttgat
     5521 caagctgaaa atttctggct tccacagcgt ggagacaacg tgtttaccgt cgcgggaaac
     5581 taagtccagt gcttgcgggc gcaagagctg cgcggaggtc cagcgagttt tcggcagttg
     5641 cagaaagata tcgacatcca gtccggtctg gtggctggca tgaccgccgt tgaaacgccc
     5701 accagcgggc atccccatat cgccaatcag caccgtaccc atgcccagat tgctcacctg
     5761 gctactcaga cgctggataa acatcaccag atccgggtga ccgaaatagc gacgctgatc
     5821 ggtacgcatg acctgataat gttcggactg tatcggcaac gtgtcagcgc cgacaataca
     5881 gccattagaa aaactgccta tcgattgtgc gctacccggc acaggttggg ttattttttg
     5941 ccacggcgtc gctgccaagc tagcgctact ggcaagcaga gccagcagcg caatcgcggt
     6001 tttgttcatt ttttaccagc gtggaatatc agtcttcaca tcggcatttt gcgcccgctg
     6061 ccgtaacagg tgatccatta aaacgatcgc cagcatcgct tcagcgatcg gcactgcacg
     6121 gatcccgaca cagggatcgt gacgaccttt ggtgatcatc tcaacttctt cgccaaagcg
     6181 gttaatagtg cgccccggca cggtaatgct ggaggtcggt ttcagcgcca tatgagcaat
     6241 gatttgctgc ccgctgctga taccgccgag aatgccgccc gcatggttgc tctggaaacc
     6301 gtctttggtg atttcgtcgc ggttctggct gccgcgcagc gccactacgt caaagccgtc
     6361 gccaatttcc acgcctttca ccgcgttgat gctcatcagc gcatgggcga tgtcggcatc
     6421 caggcggtca aataccggct cgccaagtcc ggcggggacg ccactggcaa caacggtgac
     6481 tttcgcgccg atggagtcgc cctctttttt cagcgcgcgc atcagttcat ctaacgcgtc
     6541 gattttgtcc ggatccgggc agaagaacgg attttgttca accagcgacc agtctttgat
     6601 ttccagcgga atgtcgccca tctgggtcaa gcagccgcgg atttcaatac caaatttctc
     6661 ggcgagatat tttttggcaa tcgcccctgc cgccacgcgc atggcggttt cgcgggcgga
     6721 agaacgtcca ccgccgcgat aatcgcgcag accgtatttt tgttcgtagg tgtaatcagc
     6781 atggcctggg cggaaaacgt ctttaattgc gctgtaatcc tgagagcgct ggtcagtgtt
     6841 ttcgatcaac aagccaatgc tggtgccagt agtaacgcct tcaaaaacac cagagagaat
     6901 tttgacctga tccggctcgc ggcgctgggt ggtatagcgc gatgtcccag ggcgacgacg
     6961 gtcgaggtca tgttgcaggt ccgcttccgt cagcagaatg cctggcggaa caccatcgac
     7021 gatgcagccg agcgccagcc catgcgattc gccgaaggtg gttacgcgaa agagttgtcc
     7081 aattgtgttt ccagccatca cggctccgtt attgttgtgt ttgcgtgttt acttaatctt
     7141 tataaatcgc gaaatattca cgggccgcca gcagctgctc tttggtgagc ataaacacac
     7201 catcgccgcc gttatcaaac tccagccagg tgaacggaac atccggatat tgttccataa
     7261 gatgtaccat gctgttgccg acttcacaaa tcaacacgcc atcatcagca aggtaatctg
     7321 ccgcgtttcc aagaatgcga cgcgtcagtt taaggccgtc ggtgccagat gccaggccca
     7381 gttccggctc gtgacggtat tcgtttggca ggtcggacat atcttccgcg tcgacatacg
     7441 gcgggttagt gacaatcaga tcgtactgca ctttcggcaa gtcgcggaac agatcggaac
     7501 gaatcggaat gacgttgtgg atcagaccgt gttcttcgat gttctgttca gcaaccgcca
     7561 gcgcgtcggg agagatatcc accgcgtcga cttctgcatc cgggaaggca taagcacagg
     7621 caatggcgat gcagccgcta ccggtacaca tatctaaaat atgctgcggt tgtttgctga
     7681 tgagtccggc aaatttattg ttgatcagtt caccaatcgg cgagcgcggc accagcacgc
     7741 gttcatcgac gtaaaattca tgaccgcaga accaggcttt gttggtcagg taagccaccg
     7801 gaatgcgctc gttgacgcgg cggatcacgc gttcaacaat acgatgtttt tcgctggagg
     7861 tcagacgcgc ggtgcgcata tcttccggga tatccagcgg caggtagagc gatggtaaca
     7921 ccagctgtac ggcttcatcc cacgggttat cggtgccgtg accgtaccag atatttgccg
     7981 cgctgaagcg gctcactgac cagcgcaaca tgtcctgaat ggtttgcagc tcatttactg
     8041 cttcatcaac gaaaatttta tccacgtatt cctccagggc atgatcgcaa taatttcggc
     8101 ggctagtttg ccatgaagat gacgataaat cagcattcac gcgcggtgag tgaggaaaaa
     8161 tacgtttaaa acgatcgatt gtgctacgag tcgggtaaac tgtaggaaaa ttagaaatag
     8221 agacagataa atgaaaaaga aaacaacact tagcgaggag gaccaggctc tgtttcgcca
     8281 gttgatggcg gggactcgca agattaagca ggacacgatt gtccaccgac cgcagcgtaa
     8341 aaaaatcagc gaagtgccgg tgaaacgctt gatccaggag caggctgatg ccagccatta
     8401 tttctccgat gagtttcagc cgttattaaa taccgaaggt ccggtgaaat atgttcgccc
     8461 ggatgtcagc catttcgagg cgaagaaact gcgccgcggc gattattcac cggaattgtt
     8521 tttggattta cacggtctga cgcagctgca ggccaagcag gaactgggcg cgttgattgc
     8581 cgcctgtcgc cgcgaacatg tgttttgtgc ctgcgtgatg catgggcacg ggaagcatat
     8641 tttgaagcaa caaacaccgc tgtggctggc acaacatccg catgtgatgg catttcacca
     8701 ggcaccgaaa gagtacggcg gtgatgcggc tttgttggtg ttgattgaag tggaagagtg
     8761 gttgccgccg gagttgccct gaggagttga gcgtgttgtg ccaggagcgc ggaatggcag
     8821 gggcgaatga ccctgccagg tgagaaaagg tgcgctaata catattcagt atgtaagtga
     8881 atcggcaggt agaatctatg ggctgtcgag atgtcgtgtc cacgcgtagc ttcatatcat
     8941 gcagttgcct ggacgtaatc ggttgcggct caggcgtcac cgtcactttg gcattatcac
     9001 agccggaagt cgagaaggtc atctgctgtg cgataaaacg gcagtagaca ccacgctcat
     9061 caaaattatt cccgttacac agtttgccat tttccatcag gaaagtgggc agtgtttgct
     9121 caataacacc acctgttgac tgcaacctga cgactttgtt atgtacaaca taattttcat
     9181 tccaggaaac atcatacggt gccaggggca ccgccggaca tgaagaactc accttcgttg
     9241 ggccaatata gtcaggaaaa tcatcatcat acgttttagc gaagggatct ctactcttaa
     9301 caaacgacga cacaatccac ggacaaccat gtaaattccc atcgtaataa acaaacatct
     9361 ttaattcatt acatgtccat cccgttttgg tctggctatc atactgctct aatccaggac
     9421 aggctgacat ggataccctg ctgtccgaaa ttttcgacat agatggttga gtagaactat
     9481 cgccagtcca cgaaactaaa tccggctgtg aattgggcat gaatgtagcc ttaaatatag
     9541 cgtaattaac tgaatctggc gtcgctataa tcagatgaaa ctcaaactc
//
LOCUS       DADLFA010000160         9498 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000160 DADLFA010000000
VERSION     DADLFA010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9498
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.162"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..191
                     /locus_tag="JQH65_003263"
     CDS             <1..191
                     /locus_tag="JQH65_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6140581.1"
                     /translation="AHFMMSGGNILALQKILGHHDIKMTMRYAHLAPDHLETALRFNP
                     LATLPSGDKVAAAVGITP"
     gene            complement(296..832)
                     /locus_tag="JQH65_003264"
     CDS             complement(296..832)
                     /locus_tag="JQH65_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY6140582.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            916..2124
                     /locus_tag="JQH65_003265"
     CDS             916..2124
                     /locus_tag="JQH65_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY6140583.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            2124..2939
                     /gene="ybjI"
                     /locus_tag="JQH65_003266"
     CDS             2124..2939
                     /gene="ybjI"
                     /locus_tag="JQH65_003266"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HAY6140584.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            3025..3309
                     /locus_tag="JQH65_003267"
     CDS             3025..3309
                     /locus_tag="JQH65_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6140585.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYARHLASVGEGMVIEHQIDDHHH"
     gene            complement(3350..4580)
                     /gene="mdfA"
                     /locus_tag="JQH65_003268"
                     /pseudo
     CDS             complement(3350..4580)
                     /gene="mdfA"
                     /locus_tag="JQH65_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
     gene            4865..5461
                     /gene="ybjG"
                     /locus_tag="JQH65_003269"
     CDS             4865..5461
                     /gene="ybjG"
                     /locus_tag="JQH65_003269"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAY6140586.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            5519..6277
                     /gene="deoR"
                     /locus_tag="JQH65_003270"
     CDS             5519..6277
                     /gene="deoR"
                     /locus_tag="JQH65_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HAY6140587.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPHSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(6324..7515)
                     /gene="dacC"
                     /locus_tag="JQH65_003271"
                     /pseudo
     CDS             complement(6324..7515)
                     /gene="dacC"
                     /locus_tag="JQH65_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
     gene            7809..8387
                     /locus_tag="JQH65_003272"
     CDS             7809..8387
                     /locus_tag="JQH65_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HAY6140588.1"
                     /translation="MLTLEELELPYEQILAGREFGINHDADFLAMNPNGLVPLLRDDE
                     SDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWANQTLSNAHRGILMGLV
                     RTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFGVGDIAIAPFIYNLFNV
                     GLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(8384..9475)
                     /locus_tag="JQH65_003273"
     CDS             complement(8384..9475)
                     /locus_tag="JQH65_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001450075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HAY6140589.1"
                     /translation="MPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLPDNHGMLIT
                     LRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWLSYSEVGDD
                     GKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYLFIALGENN
                     QRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQGMAMNPWSN
                     ALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAGTEQPVFYW
                     KDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRILTDRGQRI
                     RDVRTGPDGYLYVLTDESSGELLKVSPRN"
BASE COUNT         2295 a         2456 c         2344 g         2403 t
ORIGIN      
        1 cggcgcactt catgatgtcg ggtggaaaca ttctggcctt acaaaaaatt ctcggacacc
       61 acgatataaa aatgactatg cgttacgcac atctggcacc ggatcatctg gaaacggcgc
      121 tccgtttcaa tcctctggca acgctgccaa gtggcgacaa agtggcggca gcggttggca
      181 ttaccccgta ataaccacca ctgaccacca acctaactta ttgtttttaa tctaacttat
      241 tgttttcatt aacccgttta cataaatggg ttttttgtta tctaaaattt atctactacc
      301 ctgcaaccct ctcaaccatc ctcaaaatct cctcgcgcga tagcggctta cggtcggtga
      361 caaaatgcag cgtcatcccc tcaataaacg catcaagcgc gcgggcggtt ccgggttcaa
      421 accattgttc gagcgtttgc tgactgcgct gcatccagtt ttgcattacc gtttttaata
      481 gcggttttcg gctagccagc gcgtagagct ggtacatcag ctccatatta tccggcgttg
      541 caacctgtga gctgtagatc atatcggtga tagcctggca tgcgccttga gcatcactaa
      601 catcgctaaa aaatgcctga tattgccggg acataatctc agtaaaactg ctgaacgcct
      661 ccagcaacaa ctcatcaatt cctgaaaaat agtaggtcat cgatcccaac ggtaccccgg
      721 caagggtagc aattttgcgg tgcgtaacag catgtattcc gtaaagtttc accgcctcca
      781 gcgtggcctg gataattttt tctcgccgtt gcggatcgtt agcgcgacgc atgtcatttc
      841 ctctgtctgc aattgtgtac aaatgtacac aaccttgcta atgttgtctt ccctcttctt
      901 attttgacgt gatctatgac cgtaaattct tcacgtaatg cattgaaacg ccgaacctgg
      961 gcgctgttta tgttcttctt tttgccaggc ctgttaatgg cgtcctgggc aacccgtacg
     1021 cctgctatcc gcgatattct ctctgtctcg atcgctgaaa tgggcggtgt tctctttggt
     1081 ctgtcgatcg gttcgatgag cggtattctc tgctcggcgt ggttagtgaa acgctttggg
     1141 acacgtaatg tcatcctggt cacgatgtcc tgcgcattga tcgggatgat gatattaagt
     1201 ctggcactct ggctgacatc gcccctgctc tttgccgttg gtctcggcgt ctttggggca
     1261 agttttggtt ctgcggaagt ggcgataaac gttgaaggtg ccgccgttga gcgagaaatg
     1321 aataaaacgg ttttgccgat gatgcacggt ttttatagcc tgggcacgct ggcaggcgct
     1381 ggtgtcggga tggcactgac ggcctttggc gttccggcaa cggtgcacat tttattggcg
     1441 gcgctggtag gtatcgcgcc tatttatatc gccattcagg caatccctga cggtacgggc
     1501 aaaaatgctg ccgatggcac ccagcatggc gaaaaaggca ttccttttta tcgcgatatc
     1561 cagttgctgt tgattggtgt tgtagtgctg gcgatggcct ttgccgaagg ttctgccaac
     1621 gactggttac ccttattaat ggttgacggt cacggtttta gccctacttc cggctcgctg
     1681 atttatgccg gttttaccct ggggatgacc gttggacgct tcactggcgg ttggttcatc
     1741 gaccgttaca gtcgcgttgc cgtggttcgg gccagtgcac taatgggggc gttgggtatt
     1801 gggctgatta tttttgtcga tagcgcctgg gtcgctgggg tgtctgttgt actctgggga
     1861 ctgggtgcct cgctgggctt cccgctgacc atttctgccg ccagcgatac cggccccgat
     1921 gcaccaaccc gcgtcagtgt ggtagcaacg accggttatc tggctttcct cgtcgggccg
     1981 ccgctgctgg gctatctcgg cgaacattat ggattacgta gtgcaatgct ggttgtactg
     2041 gcgctggtta ttctcgcggc tattgtcgcg aaagccgtcg ccaaacccga taccaaaacg
     2101 cagacggcga tggagaatag ttgatgagca ttaaattaat tgcggtagac atggatggta
     2161 ctttcttaag cgatcaaaaa acctataacc gtgagcggtt tatggctcag tatcagcaaa
     2221 tgaaagcaca aggcattcgc tttgtggtcg ccagcgggaa tcaatattat cagttgatct
     2281 ctttcttccc tgaaattgct aatgaaattg cctttgtggc tgaaaacggc ggctgggtag
     2341 tgagtgaagg caaagatgtt tttaatggcg agctgtcgaa ggatgccttt actactgtcg
     2401 tggaacattt gctgacgcgc ccggaagtgg aaattattgc ctgcggaaaa aatagcgcct
     2461 atacgctcaa aaaatatgac gatgccatga aaacggtggc ggaaatgtat tatcaccgtc
     2521 tggaatacgt tgataacttt gacaacttag aagatatttt ctttaagttt ggcctgaatc
     2581 tttccgatga actgattcca caagtacaaa aagcattaca tgaggccatc ggcgatatta
     2641 tggtgccagt ccacaccggc aacggcagca tcgatctgat tatccccggc gtacataaag
     2701 ccaatggcct tcgccaactg cagaaattat ggggaataga cgacagcgaa gtggtggtct
     2761 ttggcgatgg cggtaacgat attgagatgc tgcgtcaggc aggctttagt tttgcaatgg
     2821 aaaatgccgg cagcgcggtc gtcgcagcgg caaaataccg ggcaggctcc aataaccgtg
     2881 aaggcgtact ggatgtgatc gataaagtgc ttaaacacga agcgccattt gaccaataaa
     2941 aacggcagca cgcccggcaa cacaatacct gaatgaaggc tgcataattc gcggaaatta
     3001 acccgctgca ttatttcagg cgaaatgatt atgaaaaatt gtctactgtt gggcgcgctt
     3061 ttaatgggct ttactggtgt ggcgatggcg caaagtgtca ccgtcgatgt gccgagtgga
     3121 tacaaagtgg ttgtagttcc tgattcagtc agcgttccac aagcggtcag cgtcgcgact
     3181 gtgccgcaaa ccgtctatgt ggctcctgcg ccagctccgg cttaccgccc gcatccttat
     3241 gctcgccatc ttgccagcgt aggtgaaggg atggttatcg aacaccagat tgacgaccat
     3301 caccattaat tcgagaatgc ctgatcgcac aaagcagtca ggcatttttt tacccttcgt
     3361 gagaatttcc catctgttta tcttttaaaa agataaccat cagcgacagc cacaaaattc
     3421 cgttgacaag gttgaagaga ttaaactgtc cgttgccccc gttcagccag gcatgtttgc
     3481 tgatttcaat accaacggta aagatcagca tttgcagcat tcccatcgcc gcagaaaccg
     3541 tacctttact catatcgctg gcaaacaggg ttaatcgcac cagtcccgca ttcgccagac
     3601 caataccgaa agcataaata cttaacccgg cagtcatcca taaatacgcg tgcgatgaga
     3661 taaccgttgc cgcagcagcg accaacagac caatcataat cggccagccg cccataatta
     3721 tcagcgaacg tacggtgcgg cgcgaggtca gacgcgctaa cagcaagtta cccgcaatta
     3781 acgccccgaa aataggcact tgcagcaagc catattcata gctgctcaac tgctcgccgg
     3841 taatgatgat aatcggcgac tgggcgatcc acgccagcaa tggcaggcta acgaatccca
     3901 gcgccagcgc ccccgccaca aagcggccgt tcttcagcac cagcttatag tcacgaccga
     3961 gttctttcag tgacagtttc tcgcctatac gcgtggcggt ttcaggcata gctcgttgca
     4021 gaccgaaaaa ggagatcgct gccaatgcgg caaacaaaac aaacatcccc tcccagggca
     4081 gcacatggat ccacgccgcg cccaccagcg gaccaagtag cggagcaatc agcgccacgt
     4141 tcgccatcag cgcggtgatc ttgatacaaa ccgcctcttc gaaagattcc tgaattgcgg
     4201 cgtatcccac agcgccaatg aaacagaggc ttatgccctg caagaagcgc aacagagtga
     4261 attgttcaat gttttgcgcc agtaatattg ccagacaggt gatgataaac cacaccaccc
     4321 ccgccagcat caccggacgg cgaccaatac gatccgacag cggccccagg agccattgta
     4381 aaaacatccc gcccgccaga tatgcggtca ttgaagtagg aacccaatca atgcccgcct
     4441 gatattgttc caccacggcc aacataccgg gttgaatcat atctgccgat ataggttgaa
     4501 aattcgtaaa gcaccagaca gagagggaaa agtaacgcct gacgtccaag cctggcaccg
     4561 gaagctaatt tattttgcat gcaatttctt cgccaataat aatcgcgcag agtttaataa
     4621 aagcgcagct aacgagaaag cgaattttgt agctgaaacc acggttaagc acattcttac
     4681 attattgcga gtatagctac gctttcttta agttttattt aacctatgcc cgttacaatc
     4741 acccaccgta aacaggccgc ttgagggaaa taagacgatg ccgctttacc cagtttaacc
     4801 tgcactttat tctcaacgac ttgcctgtat tggctccctt ttaatcactt tgcgtcggga
     4861 agttatgctg gaaaatttga atctctctct attctctctt attaacgcga cgccagactc
     4921 ggctccgtgg atgatctcgt tggcgatttt tattgctaaa gatttgatta ccgtggtgcc
     4981 gctgctggcc gcggtgcttt ggttgtgggg gcttacagca caacggcaac tggtgataaa
     5041 aatcgctatc gcgctggcgg tcagcctgtt tgtgtcctgg acgatgggac atctttttcc
     5101 gcacgaccga ccctttgtcg aaaatatcgg ctataacttc ctgcatcatg cggcggatga
     5161 ctcattccca agcgaccacg gtacggtgat tttcaccttt gcactggcat ttttatgctg
     5221 gcatcgcctg tggtccggct cacttttaat ggtgctggcc gtcgtcattg cctggtcgcg
     5281 cgtttatctt ggcgtccact ggccgctgga tatgctcggt ggattgctgg caggtatgat
     5341 tggctgcctt agtgcccaga ttatctggca agcgatgggg cataaactct atcaacgtct
     5401 gcaatcgtgg tatcgcttct gttttgcatt accgatccgc aaaggctggg tgcgtgactg
     5461 atctctgcta aaaagtgtag tattgagcgg ctcgcttcaa taactattca gagggattat
     5521 ggaaacacgt cgcgaagagc gtatcgggca gctgctgcaa gaattaaaac gcagcgataa
     5581 gttacatctt aaagacgccg ccgccctgct tggggtttcg gagatgacga ttcgtcgcga
     5641 tctgaacaac cacagtgcgc ccgtcgtttt gctcggcggc tatattgttc tggaaccgca
     5701 tagtgccagc cattacctgt taagcgatca aaaatcccgc ctggtggaag aaaaacgccg
     5761 ggcggcaaaa ctggctgcga cactggtaga acccgatcag accctctttt ttgactgtgg
     5821 caccaccacg ccgtggatta ttgaagcgat tgataatgaa atccctttta ccgccgtttg
     5881 ttattcgcta aatacctttc tggcgctgaa agagaaaccc cattgccgcg catttctgtg
     5941 cggtggtgaa tttcacgcca gcaacgccat tttcaaaccc atcgattttc agcaaacgtt
     6001 gaataatttt tgcccggata tcgcttttta ttccgcggcg ggcgtgcatg tcagtaaagg
     6061 cgctacctgt tttaatcttg aagagttgcc ggtaaaacac tgggccatgt cgatggcgca
     6121 aaagcatgtg ctggttgtcg accacagtaa atttggcaag gtccgtccgg cgcgcatggg
     6181 tgacctgaaa cgctttgata ttgtggtgag tgattgttgc ccggaagatg agtatgtgaa
     6241 gtacgcgcag acgcaccgta ttaagttgat gtattaatga cgtataaccg gatgacgttt
     6301 cgcgccatcc ggttatcaga agattaagag aaccagctgc cgaaccactg atggaatttc
     6361 atcatcacga aatcccacat cctaccaaag aatccgccct cttccacatt ttccatcacg
     6421 atcagcggac gctgctcaat ggatttaccg ttaagctgga aatcaatggt cccgacaacc
     6481 tgaccttttt tcagcggtgc ggtaagctgc ggttccgtta acgtgtagct cgccttcagg
     6541 tttttcagct gcccacgcgg tatggtcact gagcccgctt cgcctgcccc gagattcact
     6601 tcgctcttat caccaaacca gacgcgctga gtcacaaagg tggcatcagg tttaattggc
     6661 gtcacggttt caaagaagcg gaaaccccag gtcaataatt tctcagactc atcggtcagt
     6721 tttcgccccc aacactacgg agattaaacg catatcgccc tgggtagccg aagcaaccag
     6781 attatatccc gcgcctgccg tggttcctgt cttcatgcca tcaacattca gattgctgct
     6841 ccacagcaga cggttacggt taggctgacg aattttgttg aaggtgaact ctttctcttt
     6901 atgaatggcg tactcttccg gcacatcgtg gatcaatgct ttacccagca atgccatatc
     6961 gcgcgcggta ctgaactgcc ccggcgcatc caggccgtgc accgtctgga aggtagtgtt
     7021 ggtcagaccc agttttttgg cataaccatt catcagacca ataaatgact cctggctccc
     7081 ggcaacgtaa tcggccagcg caatacaggc gtcattaccg gactggataa tcacatcttt
     7141 gttcaagtct gccaccgaaa cctgatcgcc cggtttgagg aacattaccg atgaaccacg
     7201 cagtgccgga tttcccgtcg cccaggcatc tttaccgacc gtcaccatat cggtgagttt
     7261 aatcttatcg gccttaagcg cctgcccaac cacatagctg gtcatgattt tagtcaggct
     7321 cgcgggatcc agtttctcat ccgcattgcc ttctgccagc actttaccgc tggcgtaatc
     7381 cattaaaatc catgcacgcg catccacgct cggcgcttca acggtttgtt cggccgcgaa
     7441 tgccgttggg gcaaaaagga ataaaaatgc agaacccgct gcaagaccac gaaggagaga
     7501 ggagtattgc gtcataaatg ccacccgagt atccattcaa aaaaaatacg ttatatcgcc
     7561 gtatgataag ttcggtgatt aataacgcac acacaaactt aaagaaaccg taagttggta
     7621 aagtttttaa agtttatgca atatcctgcc gcttcgctgc ttctggtgtc atttttttgc
     7681 cagctgttgc ttaaaagtta actttacttc accatcggag tcctgttcag cctgtggctg
     7741 cctgtcggaa aaggggtgag tatgattacg ctgtggggtc ggaataattc aaccaacgta
     7801 aaaaagtatt gctgacgctc gaagaactgg aactacctta tgagcaaatt ctcgcgggcc
     7861 gtgagtttgg gataaatcac gatgctgatt ttctggcgat gaaccctaac gggctggtgc
     7921 cgttgttgcg tgacgacgaa agtgatctca ttctttggga atcaaacgcc attgtccgct
     7981 atctggcggc acagtacggg caaaaacgcc tgtggatcga ctcaccggca cgtcgtgcgg
     8041 aagcggaaaa atggatggac tgggcaaacc agacgctcag taatgcccat cgagggatcc
     8101 tgatgggatt agtcagaaca ccaccggaag agcgcgatca ggccgccatt gatgccagtt
     8161 gcaaagagtg cgacgccctg tttgccctgc tcgatgcgga actggcaaaa gtaaaatggt
     8221 tctctggcga cgagtttggt gtgggcgata tcgctatcgc accgttcatc tacaatttgt
     8281 ttaacgttgg cctgacctgg acaccgcgtc caaatctgca acgctggtat cagcaactca
     8341 ctgaacgccc cgcagtgcgc aaagtggtga tgattcccgt tagctaattg cgtgggctaa
     8401 ctttaagtaa ttccccactg gactcgtcgg tgagaacgta taaataaccg tcgggtccag
     8461 tgcgaacatc acgaattcgc tgccctctgt ccgttaaaat acggccatct tctgtcactt
     8521 tgtcgccgtt gacgctcatc acaatgacat ctttatcttt cagcgcgcca ataaataatt
     8581 tttgctgcca ctgggggaat ttatcgctgt tatagaaggc catgccgctc acagcgggcg
     8641 aatctttcca gtaaaaaaca ggttgctcgg tcccggcgac gatctcccct ttcgcttccg
     8701 gtatcttaaa gcctgaatag ttgattcccc aggttgccag cggccagccg tagtttttgc
     8761 ctttttgcgg gatattaatt tcatcaccac cgcgcgggcc atgttcattc agccacagtg
     8821 cattactcca cggattcatc gccattcctt gcggattacg aatgccataa gaccagatct
     8881 cggcgcgcgc accggattcc tttataaaag gattatcatc cgggatttcg ccctgatcgg
     8941 tcagacgcac cagtttgccc tgtaatttat ccagatcctg cgccgtcggg cgctgattgt
     9001 tttcgcccag agcaataaaa agataacctt taccgtcgaa taccagccgc ccgccaaaat
     9061 ggttgccggt agacagtttt ggcatctggc ggaagacggt gcggaagtcg gtcacttttg
     9121 agagatcatc acttaagcgg ccataaccca cagcagttcc ggctttgcca tcatcgccaa
     9181 cttcggaata acttaaccag atgcggcgag actgagcaaa atcaggcgct aaaaccacgt
     9241 ccagcaggcc gccctgcccg tgcgcccaaa cgtccggaac tccggaaagc ggcgcagata
     9301 atccttttcc tgcttgccag tgacgcaact cgccgccgcg cagagtgatt aacataccgt
     9361 gattatcggg taaaaaggcc agtgcccagg gatggtcgag tttgtcttgc agtacttcga
     9421 catttaccgt tgcaggagcc gcccagagag cggaagaaag acaaataagg ggcacaagga
     9481 aaaaggattg tcgatgca
//
LOCUS       DADLFA010000161         9213 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000161 DADLFA010000000
VERSION     DADLFA010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9213
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.163"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..1005
                     /locus_tag="JQH65_003274"
     CDS             <1..1005
                     /locus_tag="JQH65_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418144.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HAY6140590.1"
                     /translation="PKSEKYVFIHGPENPDETWYYDFHHRRGVIAEGGKVSNLDAMDI
                     TAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNVGVAAPFGPVNVQ
                     KQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAFIGDTLSPKGEKV
                     PELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTHHRAYPGLVNVPR
                     HWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQSAFNWHPSGEWL
                     GFVLDNRIACAHAQSGEVEYLTESHANPPSADAVVFSPDGQWLAWMEGGQLWITETDR
                     "
     gene            complement(1007..1339)
                     /locus_tag="JQH65_003275"
     CDS             complement(1007..1339)
                     /locus_tag="JQH65_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HAY6140591.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSHVEKSEANAQATNAVI
                     PPARMPDN"
     gene            1645..2058
                     /gene="ibpA"
                     /locus_tag="JQH65_003276"
     CDS             1645..2058
                     /gene="ibpA"
                     /locus_tag="JQH65_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HAY6140592.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            2170..2598
                     /gene="ibpB"
                     /locus_tag="JQH65_003277"
     CDS             2170..2598
                     /gene="ibpB"
                     /locus_tag="JQH65_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HAY6140593.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            2794..4455
                     /locus_tag="JQH65_003278"
     CDS             2794..4455
                     /locus_tag="JQH65_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HAY6140594.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAPALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(4452..5168)
                     /locus_tag="JQH65_003279"
     CDS             complement(4452..5168)
                     /locus_tag="JQH65_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY6140595.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            5463..7079
                     /locus_tag="JQH65_003280"
     CDS             5463..7079
                     /locus_tag="JQH65_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAY6140596.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMIWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGGWV
                     YTFLERILIPTGLHHFIYGQFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            7079..8402
                     /locus_tag="JQH65_003281"
                     /pseudo
     CDS             7079..8402
                     /locus_tag="JQH65_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
     gene            complement(8399..8473)
                     /locus_tag="JQH65_003282"
                     /pseudo
     CDS             complement(8399..8473)
                     /locus_tag="JQH65_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(8533..8964)
                     /gene="tnpA"
                     /locus_tag="JQH65_003283"
                     /pseudo
     CDS             complement(8533..8964)
                     /gene="tnpA"
                     /locus_tag="JQH65_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            8972..>9213
                     /locus_tag="JQH65_003284"
     CDS             8972..>9213
                     /locus_tag="JQH65_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY6140597.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQ"
BASE COUNT         2148 a         2298 c         2424 g         2343 t
ORIGIN      
        1 ccgaagtcag agaaatatgt tttcatccat ggcccggaaa atcctgatga aacatggtat
       61 tacgatttcc atcatcgacg cggagtcatt gctgaaggcg gcaaggtgag caatctcgat
      121 gcaatggata ttaccgcgcc gtatacgcca ggagcgctgc gcggcggcag ccatgtgcat
      181 gtctttagcc cgaacggtga aagggtgagc tttacctata acgaccatgt aatgcatgaa
      241 ctcgatccgg cgctggattt gcgaaacgtc ggtgttgctg cgccgtttgg cccggtcaac
      301 gtacaaaagc agcatccgcg tgaatacagc ggtagccact ggtgcgtgct ggtgagtaaa
      361 accacgccca cgccgcagcc tggcagcgat gaaatcaatc gtgcttatga agaaggatgg
      421 gtaggaaatc acgcgctggc gtttattggc gacacacttt cgccaaaggg cgagaaagtg
      481 ccggagctgt ttatcgttga gttaccgcaa gatgaagctg gctggaaagc ggcaggtgat
      541 gcgccgttaa gtggaacgga aaccaccctg cccgcgccac cgcgcggcgt cgtgcagcga
      601 cgtttaacct ttacccacca tcgggcttat ccggggttag tcaacgtccc gcgccactgg
      661 gtgcgctgta atccgcaggg tacgcaaatc gcgtttttaa tgcgtgatga taacggcatt
      721 gtgcaactgt ggcttatctc gccacagggc ggcgagccgc gccagttaac ccataacaaa
      781 acggatattc agtctgcatt taactggcat ccgtcaggag aatggttggg ctttgtgctg
      841 gataatcgaa ttgcttgtgc ccatgcgcaa agtggcgagg ttgagtattt aaccgaaagc
      901 cacgccaatc caccttctgc ggacgccgtg gtcttctcgc cggatggtca atggctggcg
      961 tggatggaag gcggccagct gtggatcacc gaaactgatc gctaaatcag ttatcaggca
     1021 ttctggccgg tggaataacc gcattggtgg cttgcgcgtt ggcttcgctt ttttcaacgt
     1081 gtgagcgcac cgagctgtct ttgcggaaca catcccacgg cagtaaaagc gtgtccatca
     1141 cagcggtaaa cggcatatcg agaatcgcca gcgacttagt gccccagttg gtttcatcgt
     1201 cgccaatcat ggttgcgctg gcgcgggtgc cgggatacgt tccttctttc cctccggtat
     1261 gggacatcac gctggagcat cctccgagta atgtcattcc gctgcatatc atgaacgcta
     1321 acaacacatt tcttatcata ataaattcat ctgttgatcg tgggtgttgg cctgatgagt
     1381 tatagcgatc ccttgctgaa aataacatca tcattacgtc gcactgtggc ggctatcgca
     1441 ctttaacgtt tcgtgctgcc ccctcagtct atgcaataga ccataaactg caaaaaaaag
     1501 tccgctgata aggcttgaaa agttcatttc cagacccatt tttacatcgt agccgatgag
     1561 gacgcgcctg atgggtgttc tggctacctg acctgtccat tgtggaaggt attacattct
     1621 cgctgatttc aggagctatt gattatgcgt aactttgatt tatccccgct ttaccgttct
     1681 gctattggat ttgaccgttt gtttaaccac ttagaaaaca accagagcca gagtaatggc
     1741 ggctaccctc cgtataacgt tgaactggta gacgaaaacc attaccgcat tgctatcgct
     1801 gtggctggtt ttgctgagag cgaactggaa attaccgccc aggataatct gctggtggtg
     1861 aaaggtgctc acgccgacga acaaaaagag cgcacctatc tgtaccaggg catcgctgaa
     1921 cgcaactttg aacgcaaatt ccagttagct gagaacattc atgttcgtgg ggctaacctg
     1981 gtaaatggtt tgctgtatat cgatctcgaa cgcgtgattc cggaagcgaa aaaaccgcgc
     2041 cgtatcgaaa tcaactaatt ccctaaggcc gcctgacgcg gcctgacatc tccatgctcg
     2101 ccgtcaggga gcatatgcga atcttcggat ttgcaggtac ttactcgctt cttagaagga
     2161 gaaatgacta tgcgtaactt cgatttatcc ccactgatgc gtcaatggat cggttttgac
     2221 aaactggcca acgcactgca aaacgccggt gaaagccaga gcttcccgcc gtacaacatt
     2281 gagaaaagcg acgataacca ctaccgcatt acccttgcgc tggcaggttt ccgtcaggaa
     2341 gatttagaga ttcaactgga aggtacgcgc ctgagcgtaa aaggcacgcc ggagcagcca
     2401 aaagaagaga aaaaatggct gcatcaaggg cttatgaatc agccatttag cctgagcttt
     2461 acgctggctg aaaatatgga agtctctggc gcaaccttcg taaacggttt actgcatatt
     2521 gatttaattc gtaatgagcc tgaacccatc gcagcgcagc gtatcgctat cagcgaacgt
     2581 cccgcgttaa atagctaact agctattctc tttgccccgc cattctggtg gggctttttt
     2641 tgtctttctt cttcaacaat gcgttaaatt tcgactgttt aagatatttc ggcacgttct
     2701 ttcccgtgat aatgtgcgct ataaggcaaa tctgcttcac cgattcagcc ataatccgta
     2761 gcaattaaaa tgttatctca agagaaatgg acgatgagtg atatagcatt aacggtcagt
     2821 attctggctt tggtggcagt cgtcggtttg tttatcggca acgtcaaatt tcgcggcata
     2881 ggattaggta ttggcggcgt gctgtttggt gggatcatcg tcggccattt tgtttctcag
     2941 gcggggatga cattaagtag cgatatgctg catgttattc aggaatttgg cctgatcctg
     3001 ttcgtttata ccatcgggat tcaagtaggg ccgggcttct ttgcctcatt gcgcgtctcc
     3061 ggattacgcc tcaacctgtt tgctgttctg atcgtcatca tcggtggtct ggttaccgcc
     3121 atcctgcata aactgtttga tattccactg ccggtagtgc tggggatttt ctccggtgcg
     3181 gttaccaata cgccagcgct gggggcaggg cagcagatct tgcgcgacct gggtacacca
     3241 atggaaatgg tcgatcagat ggggatgagt tacgcgatgg cgtatccatt cggcatttgc
     3301 gggattttgt tcaccatgtg gatgttgcgg gttattttcc gcgtcaatgt cgagacagaa
     3361 gctcagcagc acgagtcttc acgcaccaat ggcggcgcgc tgatcaagac tatcaatatt
     3421 cgcgttgaga accctaacct gcatgattta gctattaaag atgtaccgat tctcaacggc
     3481 gacaaaatta tctgctcgcg tctgaaacgc gaagaaaccc taaaagttcc ttcgccagat
     3541 accattatcc aactgggcga tttgctgcat ctggtggggc agccagcgga tttacataat
     3601 gcgcaactgg tgattggtca ggaggtcgat acttcgctgt ccacgaaagg cactgatttg
     3661 cgcgtcgagc gtgtggtggt caccaatgaa aacgtgctcg gaaaacgtat tcgcgacctg
     3721 cactttaaag aacgctatga cgttgttatc tcgcgcctga accgtgccgg ggtcgaactg
     3781 gtcgccagtg gcgatatcag cctgcagttc ggcgatatcc tcaatctggt ggggcgtccg
     3841 tccgcaattg atgccgttgc caatgtgctg gggaatgcgc agcaaaaact gcaacaggtt
     3901 cagatgctgc cagtgtttat tggcatcggg cttggcgtat tgttaggttc tattcccgtc
     3961 tttgtgcctg ggttcccggc cgcgttgaaa ctggggctgg cgggcggtcc gctgattatg
     4021 gcgttgatcc tcgggcgtat cggcagtatc ggcaagctgt actggtttat gccgccaagt
     4081 gccaacctcg cgctgcggga gctggggatc gtgctgttcc tctcggtcgt tggtctgaaa
     4141 tctggtgggg attttgtgaa taccctggtc aatggcgaag ggctaagctg gattggttat
     4201 ggtgccctga tcaccgccgt tccgctgatt actgttggca ttctggcgcg gatgttagcc
     4261 aaaatgaatt acctgaccat gtgcgggatg ctggcaggtt ccatgaccga tcctccggcg
     4321 ctggcgtttg ctaataatct tcatccaacc agtggtgcgc cggcgctctc ttacgccact
     4381 gtctatccgt tagtgatgtt tctgcgaatt atcacccccc aattactggc ggtgctcttc
     4441 tggagtatcg gttaactctt ttccgggtgg agtcgccgta aatggtactc cacctggtat
     4501 tcgctggcat ttctgaacat cactgaataa ttcaaaaact ctccgctctc gctatatgac
     4561 aacgaggtga tccgcagcag tggcgtgtgt tccgctacct tcatttgccg cgccagcagt
     4621 ctatcggcga gcaccggcgt caggctttca taattaccgc caatcaaaat cccacactct
     4681 tgttcaatat attcaaactt cgacccttcc agatgttgca gcgaaagatt acggaacagt
     4741 ttaaccggca tatagctgtc ttccagcatc agcggtttcc cttccacaaa acgaacgcga
     4801 cgggagaagt agatctgctc gttgatttga atccgtaact ggctggcaat ggccggaggc
     4861 gcaggcatga tttcaaaaat cagcacctgg ctggtgaccg tttttccctg ccgttttaac
     4921 acctccacca gtccggtcag gctggcggtt tgatgcagca catctttgcg caccaggtaa
     4981 gtgccgctgc cgtggcggcg gaccaccagc ccccacgcta ccagcaggtc aatcgcttta
     5041 cggatggtca tccgggatac tgcaaattct tccgccaact ttttttcacc gggaagaagg
     5101 ctgtttagcg tgaaatccgc ggaattaagt cgaattctta accgctccgc aatgcttttg
     5161 tagatcatct ctagatctct tttttgcctg caagcccggg taatgcgccc acaagttctg
     5221 cttaatcgat tgaaaaaaca tcttttttta aagatgtgtt cgatgctgtg taccagtgct
     5281 cacagatgtc tactttttcg cgaaaacgta gatctctacc gcccaacgaa aagcatgaaa
     5341 gcgatcacga atcccattgg gtcgtcatgt tctgccgatc gcatcttcct atcctcgctc
     5401 caggcctgcc gcataaccaa tcaggcttcc tacttacaga attgagaaaa gaggatgtgg
     5461 aaatgctcag tcaaattcaa cgctttggcg gcgcgatgtt cacgccagtg ctgctgtttc
     5521 ccttcgccgg gattgtggtg ggtcttgcca tcttgctgca aaacccgatg tttgtcgggg
     5581 aatcactgac cgatccgaac agtttattcg cgcaaatcgt acacattatt gaagagggcg
     5641 gttggacggt attccgtaat atgccgctga tttttgctgt cggtttaccc attggccttg
     5701 ctaagcaagc gcaggggcgt gcttgtctgg cggtgatggt gagtttcctg acctggaact
     5761 atttcatcaa cgcgatggga atgatctggg gaagctactt cggcgtcgat ttcactcagg
     5821 acgcggtggc aggtagcggt ctgacaatga tggccgggat taaaaccctc gataccagca
     5881 ttatcggcgc aattatcatt tccggcattg tgacggcgct gcataaccgt ctgttcgata
     5941 aaaaactgcc ggtttttctc ggcattttcc aggggacgtc ttatgtggtg attatcgcct
     6001 tcctggtgat gatcccctgt gcctggctga cgttgctcgg ttggccaaaa gtacaaatgg
     6061 ggattgaatc tctgcaagcg ttcctgcgtt cggcgggtgc gcttgggggg tgggtttaca
     6121 ccttcctcga acgtattctg atcccaaccg gtttacacca cttcatctac ggacagttta
     6181 tctttggtcc ggcagctgtt gaaggcggca ttcagatgta ctgggcgcag catctgcaag
     6241 agttcagtct gagcgccgag ccgctgaaat cgttgttccc ggaaggcggt tttgccctgc
     6301 acggtaactc aaaaatcttt ggtgccgtgg gcatttcttt agcgatgtac ttcactgccg
     6361 caccggaaaa tcgggtaaaa gtggcgggct tgctgattcc cgcaacctta accgccatgc
     6421 tggtgggaat taccgaaccg ctggaattta ccttcctgtt catttcaccg ttgctgtttg
     6481 cggtacacgc cgtgctggcg gcctcaatgt cgaccgtgat gtatctcttt ggtgtggtgg
     6541 gcaacatggg cggaggtctg attgaccagg ttttaccgca aaactggatc ccgatgttca
     6601 gcaaccacgc ggatatgatg ctgacccaaa tcgccattgg gttgtgcttt accctgctgt
     6661 acttcgtggt tttccgcaca ctgattctgc aattcaacat gtgcacgccg ggacgtgaag
     6721 atgcggaagt gaaactctac tcaaaagccg aatacaaagc ctcgcgaggc caaaccaccg
     6781 ctgcagagcc aaaaaaagag ctggatcagg ctgccggtat cctgcaagcc ctgggcgggg
     6841 tcggcaatat ctccagcatt aacaattgcg cgacgcgttt acgtattgca ctgcatgaca
     6901 tgtcacaaac gctggatgac gaagtcttta aaaagctggg agcgcacggc gtcttccgta
     6961 gtggcgatgc cattcaggtg atcattggtc tgcatgtatc ccagctgcgt gaacagctcg
     7021 atagcttaat taattctcat caatcagcag aaaatgttgc cattacggag gcagtataat
     7081 gaccaaattc tcagtggttg tcgcaggcgg tggaagcacc tttacgccag gcatcgtgtt
     7141 gatgctcctg gcgaatcagg accgtttccc gcttcgtgca ctgaaatttt atgataacga
     7201 tggtgcgcgg caggaagtga ttgccgaagc ctgtaaagtc atccttaaag agaaagcgcc
     7261 ggacattgcg tttagttaca ccaccgatcc tgaagtggca ttcagcgacg ttgattttgt
     7321 catggcgcac atccgcgtcg gcaaataccc gatgcgcgaa ctggatgaaa aaatcccgct
     7381 gcgccacggc gttgttggtc aggaaacttg cggacccggc ggaatagcgt acggcatgcg
     7441 ttccattggc ggcgtcctgg aactggtgga ttatatggaa aaatattcac caaatgcctg
     7501 gatgctcaac tactccaacc cggcagcaat tgtcgcagaa gccacgcgtc gtctgcgccc
     7561 gaatgcgaaa atcctcaaca tctgtgacat gccaatcggt attgaaagcc ggatggcgca
     7621 aattgttggg ctgcaagatc gcaaacagat gcgcgtgcgc tactacggcc tgaaccactt
     7681 tggctggtgg acatcaattg aagatttgca gggcaacgac ctgatgcccc agctgcggca
     7741 atatgtctct aagcacggtt atgttccacc gcagcaagat acacatactg aagcgagctg
     7801 gaacgacacc tatgcaaaag cgcgggatgt ccaggcactg gacccggata cattgccgaa
     7861 cacctatctg aaatattatc tcttcccgga ttatgttgtt cagcattcca accctgaaca
     7921 tacccgcgcg aatgaggtga atggaacatc gcgagaaaca ggttttcgat gcttgccgcg
     7981 ccattacagc ggcaggaaat tcagcggcgg ccaagctgga gattgacgaa catgcgtcat
     8041 acatcgtcga tctggcggcg gcaattgcct tcaacactca ggagcggatg ttgctgattg
     8101 tgcctaacaa cggggcaatc cataactttg atgatgaagc gatggtcgag atcccgtgtc
     8161 tggtcgggca caacggacca gaaccactgg tggtcggcga tatcccgcag tttcagaaag
     8221 ggttaatgag tcagcaagtg gcggtggaaa aactggtcgt ggacgcctgg gaacagcgtt
     8281 catatcagca cctgtggcag gcgattacgt tatcgaaaac ggtaccgagc gcctcggtcg
     8341 ccaaagctat tctggatgag ttgctggagg ccaacaaagc gtactggcca gagttacgtt
     8401 aattgtgtgg actggcatcc gttgggtgct ggtccgtatt ctttagcacg gctgacggcg
     8461 cataaccaaa tttatccggt gcgccgtaaa accccgtcct tcagggcggg gatataaggc
     8521 gcggttttcc acctaaccag gtgtttgctg ttgttcaatg tattggcgga tgacggatat
     8581 tggcgcacct ccgcagctat tggcaaaata gccaggactc cacaaaacgc ctttgtagta
     8641 atacctgact gcaatatctg gtcgatctcg tcgcagtaac ctacccgata cgcctttgag
     8701 gctgtttacc agactggata tcgccagttt gggaggatag ttaattaaca aatggacgtg
     8761 atctggttcg ccatccattt aaaccagttc agcttcaaaa tcagcacata catttgaaaa
     8821 gtaagtgcgt agtttttctg tcgcgtcata gtcaaaaatc tgacgtcggt atttggtgac
     8881 aaagaccagg tgaacatgca tcaggaaaac acaatgcctt ccacgccgga tatcggtttc
     8941 ttttttcaca gaccaaagta tcatttggcc tgtgaaacga ctacaagcat ttaaattcca
     9001 gttaagaccc ggtggtcaac aggagcgtga aatgaggcgc ttcgcaggcg catgtcgttt
     9061 cgttttcaat cgtgctctgg cacgtcagaa tgagaatcat gaggccggta ataaatacat
     9121 cccttacggg aaaatggctt cctggctggt tgagtggaaa aatgccactg aaacgcaatg
     9181 gcttaaagat gccccgtcac agccattgca aca
//
LOCUS       DADLFA010000162         9089 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000162 DADLFA010000000
VERSION     DADLFA010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9089
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.164"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            52..936
                     /locus_tag="JQH65_003285"
     CDS             52..936
                     /locus_tag="JQH65_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HAY6140598.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFCPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSISMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(987..1841)
                     /gene="yeaE"
                     /locus_tag="JQH65_003286"
     CDS             complement(987..1841)
                     /gene="yeaE"
                     /locus_tag="JQH65_003286"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HAY6140599.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGRQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISSAQVLLARVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(1931..2677)
                     /locus_tag="JQH65_003287"
     CDS             complement(1931..2677)
                     /locus_tag="JQH65_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MltA-interacting protein"
                     /protein_id="HAY6140600.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            3113..5047
                     /gene="yeaG"
                     /locus_tag="JQH65_003288"
     CDS             3113..5047
                     /gene="yeaG"
                     /locus_tag="JQH65_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HAY6140601.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            5160..6443
                     /locus_tag="JQH65_003289"
     CDS             5160..6443
                     /locus_tag="JQH65_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HAY6140602.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAMNKRSVT
                     DVDSGESISIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNALARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            6590..8065
                     /locus_tag="JQH65_003290"
     CDS             6590..8065
                     /locus_tag="JQH65_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAY6140603.1"
                     /translation="MIQSTRISMGLFFKYFLSLTKIDPGQNYISLPSIKPSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATRGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIADRIRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
     gene            8159..8245
                     /locus_tag="JQH65_003291"
                     /pseudo
     CDS             8159..8245
                     /locus_tag="JQH65_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459804.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            8246..>9089
                     /locus_tag="JQH65_003292"
     CDS             8246..>9089
                     /locus_tag="JQH65_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY6140604.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGREVAK
                     FSVPL"
BASE COUNT         2392 a         2182 c         2109 g         2406 t
ORIGIN      
        1 cttcggtcgc tctttttttt acctgataaa atgaagttaa aggactgcgt catgattaag
       61 aaaatttttg cccttccggt catcgaacaa atctcccctg tcctctcccg tcgtaaactg
      121 gatgaactgg acctcattgt ggtcgatcat ccccaggtaa aagcctcttt tgcattacag
      181 ggcgcacacc ttctctcgtg gaaacctgcg ggtgaagaag aagttctgtg gttgagcaac
      241 aacacaccgt tcaaaaatgg cgtcgctatt cgcggtggcg taccggtttg ctggccgtgg
      301 tttggtccgg cggcacaaca aggtctgcct gcgcacggtt ttgcccgcaa cctgccgtgg
      361 acgctgaaat cacatcatga agatgctgat ggcgtagcgc tgacttttga attgacgcaa
      421 agcgaagaga cgaaaaaatt ctgtccgcac gactttacgc tgttagcgca tttccgcgtg
      481 ggtaaaacct gtgaaatcga tcttgaatca catggcgaat ttgaaaccac ctctgccctg
      541 catacctact ttaacgtggg tgatatcgct aaggtaagcg tcagtgggct gggcgatcgc
      601 ttcattgata aagtgaatga cgcgaaagaa gatgtactga ccgacggtat tcagaccttc
      661 cctgaccgta ccgatcgcgt gtatctgaat ccgcaagatt gcagcgtgat taatgatgaa
      721 gcgctgaatc gtattatcgc cgtaggccac cagcatcatc tgaacgttgt aggctggaac
      781 ccgggaccgg cgctttcaat tagcatgggc gatatgccgg atgatggcta caaaacattt
      841 gtttgtgtag aaacggctta cgcttcagaa acgcaaaaag tcaccaaaga gaaacctgca
      901 catctggcgc aatccattcg cgttgcgaaa cgttaatttg cgttaatgtt gtgtgccggg
      961 tgcgatgcat ccggcacgta tcaacatcac accatatcca gcgcagtttt tccttttggt
     1021 gccggatacg ctttatccag catggttaat tccgctgacg aaagttcgac ctcaagcaca
     1081 gccgcattct gttgaacatg ggcaatcgtg gccgcttttg gaatcgccat cacaccctga
     1141 tggctgatta ccctcgccaa caatacttgt gccgagctga tattgtgagc atgtgcaatt
     1201 tcgttgacta ccgcgttttt taacagtcca ttgcgcaacc gcccggcctg ggctaatgga
     1261 ctgtaagcca tcaccggcat ctgctgttgc tggcaccagg ggagtagatc gtactcaatc
     1321 cctcgtgaac cgagatggta aagcacctga ttagtggcac actgatttcc ccccggcagc
     1381 tgccagagtt cctgcatatc agcataatca aggttagaaa cgccccagcg gcggattttt
     1441 ccctgggcga tcaatttttc catcgctgca acagtctctt caaaagcgaa actgccagac
     1501 cagtgtaata agtagagatc gagataatca gtattgagac ggcgtaaact ggcttcgcat
     1561 gcatttatcg ctttttgcct gccagcattc cacggataga ctttagagac gagaaagacg
     1621 ttatcacgca gaccggttaa tgcttcccca accacctttt cggcaccgcc atcggcatac
     1681 atttcggcgg tatcaatgag ggttaaaccg agctcaatgc ccgcgcgtag tgcagcaact
     1741 tctgttttgc gctgactggc atcttcgccc atataccatg ttccctgccc tatggctggc
     1801 agtgagacat cgccactaaa ttgaatcatt ttttgttgca ttgtttcctc ccaggtattg
     1861 caccaccgta atgcaaaaca gggcgtgacg ccctgttttt atgcacaaaa tgccctggaa
     1921 agatgcatta tcagaatttg taggtgatcc cggtagaaat caggccagtc caggatttat
     1981 ccaccatcgg gctgtcagta acttcatcag acagacgggt atagcgcgcg gtaccgtaaa
     2041 cactccagtc gccgaggaag ttgtagctgg cgctcagctc caggtaaggg ctccagctgt
     2101 cgttcgggtt atagccacgc agaccgctgc gagcggactc tttgcgcgat acgccataat
     2161 agtattcgtt ctggttttcg ctgttccact gcacaccaat acccggagtc acggtcaggc
     2221 caccgttggt gtaacgatac aaccaggcca tatcccagac aatgccgttg ctgttatcca
     2281 gggtatcgcc agccagggtg gtacgcaggt aaccgtactg ggtaaagtga gcataagaca
     2341 gaccagccat catggtgctc ttacggtcat ccaggtgacg catttggtga tcgccactgt
     2401 ctttcgcttt gaagtaaagc ggcgaccagt aagcggtaat tgaaagttta tccgttgcgt
     2461 cattccacag gtagtaacca ccacctaagc cacggaacca gaagttatcg ccttcatagt
     2521 tgattaccgg tactgggtaa acatcggtat cgtaatcttt atatgggtgc tcaacgacac
     2581 ctacgcctgc gcccaaggaa aatttacctt cagcgtgcgc tacgcctgca gacgttgcga
     2641 taagcactcc aagtgccaga agtttgagtt tggtcacaat taatcattcc ttaaacaaat
     2701 gtttagcggg cgacaaagtt tacccgtcaa tacatcgaaa cccaaccttt ttacgttttc
     2761 attttttaaa gtaactgttt aattttcctg ccgcggatga caccgcgctt acagccaaat
     2821 gaatttagcg ttactggcga gcctggtctt tacattaatt atgcaaaatt tatggatgag
     2881 ttgttgatat gccattgaaa ttaagaaagc cgtgcaggca agttttccat ttgccatcta
     2941 cgcttaattt tgaaggtgta tcaccgggca cgttgttctc atcgtcgata aaatggcatg
     3001 agagttgctg tgttttagca agagacgtcg ttcagtttac ctcttccggg aggctctact
     3061 attcatatga acggctctta acctgtgcta aaaaacgaaa ggacggcata ccatgaatat
     3121 attcgatcac tatcgccagc gatatgaagc tgccaaggac gaagagttca cactgcagga
     3181 gtttcttacc acttgtcggc aagatcgcag tgcttatgcc aacgcggctg agcggctatt
     3241 gatggctatc ggtgagcctg tcatggtcga tacagcccag gaacccagac tttctcgact
     3301 cttttctaac cgggtcattg cacgttatcc ggcgtttgaa gagttttacg gcatggaaga
     3361 cgcgattgaa cagattgtct cttatctgaa acacgcggct caggggctgg aagagaagaa
     3421 acaaatcctg tatctgctgg ggcctgtggg tgggggtaaa tcatcgcttg ctgagcgact
     3481 gaaatcatta atgcagctcg taccgattta tgtattgagc gccaacggtg agcgtagccc
     3541 ggtcaacgat catccgttct gtcttttcaa tccgcaggaa gatgcgcaga ttctggaaaa
     3601 agagtatggc attcctcgcc gttatctcgg caccatcatg tcgccgtggg cggcaaaacg
     3661 cctgcatgaa tttggtggcg atatcactaa gttccgggta gtgaaggtct ggccgtcaat
     3721 tctgcaacaa attgctatcg ccaaaacgga acccggcgat gagaacaacc aggacatctc
     3781 cgcgctggtt gggaaagtcg atattcgtaa actcgaacac tacgcgcaga atgacccgga
     3841 cgcctacggc tattccggtg cgctgtgccg cgccaatcag gggatcatgg aattcgttga
     3901 gatgtttaaa gcaccgataa aagtgctgca tcccttgtta accgccactc aggaaggtaa
     3961 ctacaacggg acggaaggta tctccgccct gccgttcaac gggattattc tcgcccactc
     4021 gaacgagtcc gaatgggtca ctttccgtaa taacaaaaac aacgaagcct tcctcgaccg
     4081 tgtttacatc gtgaaggtgc cgtattgctt gcgcatttcc gaagagatca aaatctacga
     4141 gaaattgctt aatcacagtg aattgactca cgccccatgc gcccctggca cgctggaaac
     4201 actgtcacgt ttttccattc tttcgcgcct gaaagagcca gaaaactcca gcatttattc
     4261 aaagatgcgg gtttatgatg gcgaaagtct gaaagacacc gatcccaaag ccaagtcgta
     4321 tcaggaatat cgtgactacg ccggtgtcga tgaagggatg aacggtctgt cgacgcgttt
     4381 tgcgtttaag atcctctccc gcgtgttcaa cttcgatcat gtagaagtgg cggcaaaccc
     4441 ggtccatctg ttctacgtcc tggaacagca gatcgagcgc gagcagttcc cgcaagagca
     4501 ggcagaacgc tatctggagt tcctgaaagg ttatctgatc ccgaaatatg ccgagtttat
     4561 cggcaaagag atccagacgg cctaccttga atcctattcc gaatatgggc aaaacatttt
     4621 cgaccgttat gttacctacg cggatttctg gattcaggat caggagtatc gcgatccgga
     4681 taccgggcag ctgtttgacc gcgagtctct taacgccgag ctggagaaaa tcgagaaacc
     4741 ggcggggatc agtaatccaa aagatttccg caacgagatt gttaacttcg tactgcgcgc
     4801 cagagcgaat aacagcggac gcaatccgaa ctggaccagc tatgaaaaac tgcgcacggt
     4861 tatcgagaag aaaatgttct ccaataccga ggagctgttg ccggttatct cgtttaacgc
     4921 caaaacgtca accgacgagc agaagaaaca cgacgacttt gtcgaccgta tgatggaaaa
     4981 aggctacacc cgtaaacagg tgcgtttact gtgcgaatgg tatttgcgcg tacgtaaatc
     5041 gtcttaacaa ccctggcccg gtacacatgc taccgggcct acaacgacag cgaaccgtgg
     5101 gccagagaaa tggcaacaca ggcgtagcat acagttggca aatgtagtac ggggggcata
     5161 tgacctggtt tattgaccgg cgtctgaacg gcaaaaacaa aagcatggtg aatcgccagc
     5221 gttttttacg ccgttataaa gcgcaaatta aacagtcgat ctccgaggcc atgaataagc
     5281 gttcggtgac tgacgtcgac agcggcgaat ccatatccat tcccacggaa gatattagcg
     5341 aaccgatgtt tcatcagggg cgtggcggcc tgcgccaccg cgtacatccg ggcaatgacc
     5401 atttcgtcca gaacgaccga attgaacgtc cccagggtgg cggcggaggt tccggcagtg
     5461 gtcagggcca ggccagccag gatggtgaag gtcaggatga atttgtcttt cagatttcga
     5521 aagatgagta tcttgatctg ctctttgaag atttggcctt accgaatctg aaacaaaacc
     5581 aacaacgcca gctgaccgaa tataaaacgc atcgggcggg ttataccgct aacggcgttc
     5641 cagccaatat cagcgttgtg cgttccttgc agaacgcact ggcgcgacgc acagccatga
     5701 cggcgggcaa gcgacgggaa cttcatgcgc tggaagagaa tttggccatc atcagcaaca
     5761 gtgaacctgc gcaactgctg gaagaggaac gtctgcgcaa agaaattgca gaattacgtg
     5821 ccaaaattga acgcgtccct tttattgaca ccttcgattt acgttacaag aactacgaga
     5881 agcggcccga tccctccagc caagcagtga tgttttgcct gatggacgtt tccggttcaa
     5941 tggatcaatc cactaaagat atggctaagc gtttttatat tctgctgtat ctgttcctca
     6001 gcagaacgta taagaacgtg gaagtcgtat acatccgcca tcatacccaa gcgaaagaag
     6061 tcgatgaaca tgagtttttc tactcgcagg aaacaggcgg caccattgtt tccagcgccc
     6121 tgaaactgat ggatgaggta gtgaaagagc gttataaccc ggcacagtgg aatatttacg
     6181 ctgcacaagc atcggacggc gataactggg ccgatgactc tccgctttgc catgaaatcc
     6241 tggcgaaaaa attattacct gttgttcgtt attacagcta tatcgaaatt acccgtcgtg
     6301 cacatcagac attgtggcga gaatatgagc atctgcaatc tactttcgac aactttgcga
     6361 tgcagcacat ccgcgaccag gatgatattt atccggtgtt ccgtgaactg tttcataaac
     6421 aaaatgcaac agctaaagac taaaactatc agccaggtca ttatcgcctg gctgattttt
     6481 agcttactgt aaattatctc atttattaca tacattagct tacaatcgct ttaaatatga
     6541 cagcataacc tttacataat ttagttccag aaaacaatca ttcggaaaaa tgattcagtc
     6601 aacacgtatt tccatggggt tattctttaa atatttttta tcgttaacga aaattgatcc
     6661 tggtcaaaac tatatatctc tgccatcaat aaaacccagc actcacattg ctctcctttt
     6721 tatggtttct atgggtacac aaaaattaaa agctcaaagc ttttttattt tcagtttatt
     6781 gctgacatta attttatttt gcattactac cttatataac gaaaacacaa atgtaaaact
     6841 catcccacag atgaattacc tgatggttgt tgtggctttg tttttcctta acgccgtcat
     6901 ttttcttttc atgttaatga aatatttcac taacaaacaa attttaccaa cactcatttt
     6961 aagccttgca ttcttaagtg gccttatcta tttagttgaa accattgtaa ttatccataa
     7021 accaattaac ggcagtacac tgatccagac aaagtcgaat gatgtttcta ttttctatat
     7081 tttccgccaa ctcagtttta tttgtttaac ctcgctggca ctcttttgtt atggaaaaga
     7141 caacatcctt gacaacaata agaaaaaaac gggaatcctg ttgctggcgt tgattccttt
     7201 tttagttttt ccccttctgg cacacaatct gagcagttat aacgctgact attctttgta
     7261 tgtcgtcgac tactgtccgg acaaccatac tgcgaccagg ggaatcaact atacaaaaat
     7321 attggtttgt ctgtgggcat ttttactgtt ctttattatc atgcgtacac gattagccag
     7381 cgaactatgg ccgttaatag cattattatg tctggcatcg ctatgctgca acttacttct
     7441 actgactctg gatgagtata attacaccat ctggtatatc agtcgcggga ttgaagtttc
     7501 cagtaaactg tttgttgtgt cttttctgat ttataacatt tttcaggagc tgcaactctc
     7561 cagcaaactg gcagttcatg atgtgctgac caatatttat aatcggcgct actttttcaa
     7621 cagcgtagag tcattattgt cgcgacctgt tgttaaggac ttctgtgtca tgctggttga
     7681 tattaatcag ttcaaacgca tcaatgccca atggggacat cgtgtgggtg ataaagtgct
     7741 ggtttcaatt gtcgatatta tccagcaaag catccgccct gatgatattt tagcgcgact
     7801 ggagggtgag gtgtttggct tgctatttac cgaactcaat agtgcccagg caaaaatcat
     7861 tgcggatcgt atacgtaaaa atgtcgaact cctgaccggc tttagtaaca gatatgatgt
     7921 tcctgaacaa atgaccatca gtattggcac ggttttttca acgggtgaca cgcgtaatat
     7981 ctcgcttgtc atgacggaag cagataaagc cttacgcgaa gcgaaaagcg aggggggcaa
     8041 caaagtgatt attcatcata tttaagtgca aaaatattca gagccatgct ttttgcgtgg
     8101 cttttgcata caatttatta ctattacccc taaatttccc ctccccagcg gcgcagagat
     8161 gagtataatt agcgcccctg tgccaggccg caatcgaact ttatctggtt ttctcgtttc
     8221 actaactgaa ggagtgccat ttatcatgaa attgcaccat agaatgctcc ggcattttat
     8281 cgccgcaagt gtcattgtgc tgacatcttc cttccttatt tttgaacttg tcgccagcga
     8341 cagagcaatg agtgcctatc tgcgctatat cgtgcagaaa gcagactcct cctttcttta
     8401 tgataagtat cagaatcaga gtattgccgc gcatgtgatg cgcgctctcg ctgctgagca
     8461 gtcggaagtg tcgccagaac agcggcgcgc catctgcgag gcttttgagt ctgccaataa
     8521 tacccatggc ttaaacctga ctgctcataa atacccgggc ttacgcggca cactacaaac
     8581 cgcatccact gactgcgaca caattgtgga agctgcagca ctattacccg cttttgatca
     8641 ggcagtggaa ggcaaccgcc accaggatga ttacggttca ggtcttggga tggccgaaga
     8701 gaaatttcac tattatctcg atctcaatga ccgctatgtc tatttttatg agccggttaa
     8761 tgttgaatac tttgcgatga ataactggtc cttcctgcag tcaggaagta ttggtatcga
     8821 tcgcaaagat attgaaaagg tatttaccgg gcgtaccgta ttgtcgagca tttaccagga
     8881 tcagcgtact aaacagaacg tgatgagttt gctgacgccg gtgtatgttg cagggcagct
     8941 aaaagggatt gtgctgctgg atattaacaa aaacaatttg cggaatatct tttacactca
     9001 tgaccgccct ctcctctggc gttttctcaa tgtcacgcta accgataccg attcggggcg
     9061 tgaagtggcc aaattcagtg tgccacttc
//
LOCUS       DADLFA010000163         9010 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000163 DADLFA010000000
VERSION     DADLFA010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9010
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.165"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..984)
                     /locus_tag="JQH65_003293"
     CDS             complement(<1..984)
                     /locus_tag="JQH65_003293"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="PtrB; oligopeptidase that cleaves peptide bonds
                     following arginine and lysine residues; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HAY6140605.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRLRDEQQWEELIPPRENIMLEGFTLFTDWL"
     gene            complement(1193..1852)
                     /locus_tag="JQH65_003294"
     CDS             complement(1193..1852)
                     /locus_tag="JQH65_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HAY6140606.1"
                     /translation="MANWLNQLQSLLGQSSSSISSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(2179..2535)
                     /gene="yebF"
                     /locus_tag="JQH65_003295"
     CDS             complement(2179..2535)
                     /gene="yebF"
                     /locus_tag="JQH65_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HAY6140607.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(2602..2896)
                     /gene="yebG"
                     /locus_tag="JQH65_003296"
                     /pseudo
     CDS             complement(2602..2896)
                     /gene="yebG"
                     /locus_tag="JQH65_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            3030..4208
                     /gene="purT"
                     /locus_tag="JQH65_003297"
     CDS             3030..4208
                     /gene="purT"
                     /locus_tag="JQH65_003297"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HAY6140608.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEERLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVADIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWKYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCSDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(4264..4905)
                     /locus_tag="JQH65_003298"
     CDS             complement(4264..4905)
                     /locus_tag="JQH65_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAY6140609.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(4942..6753)
                     /gene="edd"
                     /locus_tag="JQH65_003299"
     CDS             complement(4942..6753)
                     /gene="edd"
                     /locus_tag="JQH65_003299"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020244583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HAY6140610.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQSPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(6988..8463)
                     /gene="zwf"
                     /locus_tag="JQH65_003300"
     CDS             complement(6988..8463)
                     /gene="zwf"
                     /locus_tag="JQH65_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAY6140611.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            8801..>9010
                     /locus_tag="JQH65_003301"
     CDS             8801..>9010
                     /locus_tag="JQH65_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiR family transcriptional regulator"
                     /protein_id="HAY6140612.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHL"
BASE COUNT         2145 a         2353 c         2241 g         2271 t
ORIGIN      
        1 cagccagtcg gtaaacagcg taaacccttc cagcatgatg ttttcgcgtg gcggaattaa
       61 ctcttcccac tgttgctcat cacgcaagcg ggtacggtat aagccaaagt ttttgccgtg
      121 gcggttggaa cgcagataaa accgatgctg gtagtgatca aggctgtatt cgtgatcttt
      181 gcggcgcggc agaaaaacaa acggctcggc atcggccatt tccgcgtcca gcaggcgaac
      241 ttcactggtg gtggcgctgg ccaaatgaat gactacatag tgcttcgagg tcgttttatg
      301 caggctgacg taataggtat cgtctttttc ttcgtagatc agtttatctt gcgatgctgg
      361 cgtaccgatg gcgtgacgcc agacctgata aggcagcagc gtcaccggat gcttgcgaac
      421 atagtagaaa gtccaggagt catttgccca gacaaagctg ggttcaacgt tatccagcag
      481 ttccgggtac cagttaccag tttccagatt acgaaaacga atgccgtact ggcgtcggga
      541 aagaaaatct tctgccagcg ccataatggt gttatcgggc gtaatcgcca ttccgcccat
      601 cgaataaaac tcactatgag ctgcgcgctt attggcatcg agcaatgttt cccactcatc
      661 ccactcttca ctgaatgccg attgacgctg gtagatagca tattcacagc ctggttcata
      721 aatatgccga tagcggtagc catttttgat gtagggcgca gaaacttctc gttgcggaat
      781 gcggtcgatg atttccttta agatgcgatc ctgcaaggct tgttgtgagg ccatcacccg
      841 atgaccgtaa ctattttctt gttgcaggta gtccaggact tctggctgag aacgcgtatc
      901 gtcccgcagc cagtagtaat tatcgatgcg cgtatcgcca tgaagcgtca tggcgtgggg
      961 aatgcgggcg gcttttggta gcatgttatt gttctttctg gttgaaacat cttataaggg
     1021 tggcaaaact caccggggat gcaagcgaaa caggggagtc attgcttaga tgatgacagg
     1081 taatggcgcg gatatcgaat gttatgcaga cagagaaaat cagcctgttc aaatggctgt
     1141 gcgattctgg atagcccgaa atagtcaact tcaggctatc cagagagcgg aattattccg
     1201 ccaaagtgcg tttttgctgt tcgagatcgc gttcaatgcc gtcacgaaca tcctggggga
     1261 ttttcagcgc gtcacccagt gcattcaggt aactgcgttc cataaaatgg tcaatatcaa
     1321 tagccgcgca actcaggaaa tagatttcca gcgcctcttc ttcattgcgg accccggtag
     1381 ccaagcgttg tggatccagc ggttgttcga ttgcctgctc aatgagtaca cgcccctgct
     1441 cttccacgcc ggcttcacgc aattgctggt cgattgccgc acgttctttg gcatcaatat
     1501 gaccatcact tttagcggca aagaccagcg caaggatcaa acgttctgta cgctcatcca
     1561 gcggcgtact ttgcgcgcca aactgcggtt cgtcctgatg cgccgcgcga attttatctt
     1621 tgtatttatt ccacagcacc gtacccgcta ccgctccgcc gccaaccagt aacgcgttgg
     1681 tgccatattt cgtaagaagt ttacgtgctg atttatttgc gaccagcagc ccagccagtc
     1741 cgcctaatgc gcctggcact aacagtttgc ccaatccctg atccgcagac gaggagatag
     1801 aagaactgct ttgcccaagc agggattgca gttgatttaa ccagttagcc atatttgctc
     1861 ctcaataacc atttttagtg cctgtagcat agcggatgag gatgtcagaa aatgtatatc
     1921 gagacaaaag atgcgcaaat aagcggggcg gtaggccgtt attcaaagaa attcgcgcca
     1981 ggtaaggtat ttactgtata tcaggtgata agggtatttt aggtgaaagt agtttgtcgt
     2041 tttgagtggc attgtttgat gctgattgcc cgatgcgacg ctgttgcgtc ttctcaggcc
     2101 tacgattgtg gtgcgttctg gggcggacaa ggcactcgcg ccgcatccgc caacggtcaa
     2161 tcgaattatc agaagccatt aacgccgctg atattccgcc attcccgctt tgcagtccac
     2221 gctgacctgg taatgaatat cggcactttt accacgcacg gttagcggta ctgaccattt
     2281 atcatcttta ccctgaatgt cctgcaaact gacccaagcc acgggatcgg cctgaccgac
     2341 aattttttga tcatctgccc aacgcgccac gcgattttgt tgataatcac gttttacgct
     2401 cgcggcaatt ccggcagcat ccagaccttc acactttggg aaagtgaccg acttgctggt
     2461 ttcattattg gcagcgaaaa ctgatgcgca ggcagaaacc aacaacagcc ctaaaaacgc
     2521 ccctcttttt ttcatgtttt tctccatagc acaatgattc aggagaaagc atggtacaaa
     2581 ttgtcaggag cgcaagttgc ttcaggccgc gtgtgaggca tcttcctctt cggattcagc
     2641 accgacgacc tgtgggtggg gacggtaatt tgccggtttt cagaatggtg ctcagcacat
     2701 ctttttgttc tgccagccat agcgaaaggg cttcacgttg ctcgtcttcc atttgcactg
     2761 gagaattagt cagccaggtg tcaagcagat ccgccgtatc aagcattttg tcataagcat
     2821 cggcttcctt tttgctggta aacgacattt tctcttcgcc ctcacgaatg actacgtatt
     2881 taacttcaac cgccatttgc agcctctcat aataactgtg attttataca gtatatttct
     2941 tttcggttga gaaatcaaaa tcaacaataa agacacacgc aaacgttttc gtttatactg
     3001 cgcgcggaat taatcagggg atattcgtta tgacgttatt aggcactgcg ctgcgtccgg
     3061 cagcaactcg cgtgatgtta ttaggctccg gtgaactggg taaagaagtg gcaatcgagt
     3121 gtcagcgtct cggcgtagag gtgattgccg tcgatcgcta tgccgacgca ccagccatgc
     3181 atgtcgcgca tcgctcccat gtcattaata tgcttgatgg tgatgcatta cgccgtgtgg
     3241 ttgaactgga aaaaccacat tatatcgtgc cggagatcga agctattgcc accgatatgc
     3301 tgatccaact tgaagaggaa agactgaatg ttgtcccctg cgctcgcgca acgaaattaa
     3361 cgatgaatcg cgagggtatc cgtcgcctgg cggcagaaga gctgcagctg cccacttcca
     3421 cttatcgttt tgccgatagc gaaagccttt tccgcgaggc ggttgctgac attggctatc
     3481 cctgcattgt aaaaccggtg atgagctctt ccggcaaggg gcagacgttt attcgttctg
     3541 cagagcaact tgctcaggca tggaagtacg ctcagcaagg cggtcgcgcc ggagcgggcc
     3601 gcgtaattgt tgaaggcgtc gttaagtttg acttcgaaat taccttgcta accgtcagcg
     3661 cggtggatgg cgtccatttc tgtgcaccgg taggtcatcg ccaggaagat ggcgactacc
     3721 gtgaatcctg gcaaccacag caaatgagcc cgcttgccct tgaacgtgcg caggagattg
     3781 cccgtaaagt ggtgctggca ctgggcggtt atggtctgtt tggtgtcgag ctgtttgtct
     3841 gtagtgatga ggtgattttc agtgaggtct cccctcgtcc acatgatacc gggatggtga
     3901 cgttaatttc tcaagatctt tcagagtttg ccctgcatgt acgtgccttc ctcggacttc
     3961 cggttggcgg gatccgtcag tatggtcctg cagcttctgc cgttattctg ccacaactga
     4021 ccagtcagaa tgtcacgttt gataatgtgc agaatgccgt aggcgcagat ttgcagattc
     4081 gtttatttgg taagccggaa attgatggca gccgtcgtct gggtgttgcg ctggcgaccg
     4141 ctgagagtgt tgttgacgcc attgaacgcg cgaagcacgc cgccggacag gtaaaagtac
     4201 agggttaaac ccggcaaaaa aacgctacaa aaatgcccga tcgtagatcg ggcgttttga
     4261 cttttacagc ttagcgcctt ctacagcttc acgcgccagc ttagtaatgc ggtcgtaatc
     4321 gcccgcttcc agcgcatctg ccggaaccag ccaggaacca ccgatgcaca gcacgctttt
     4381 cagcgccagg tagtcacggt agttagccgg agaaatacca cccgtcgggc agaaacggac
     4441 ctgggagaac ggacccgcga tcgcctgcag ggctttcacg ccgccgttag cttcagccgg
     4501 gaagaatttg aactctttca aaccgtagtc catacccagc atcagttcgg aaacagtgct
     4561 gatccccgga attagaggaa tagtcccttc ggtagcagct ttcagcagcg gctcggtcag
     4621 acccgggcta attgcgaact gtgcacccgc ttcagtgact tctgccagct gctgtggatt
     4681 cagcaccgta ccggcaccca caatcgcttc aggcacttct ttggcgatag cacggatagc
     4741 gtcaactgca cactcggtac gcagagtcac ttccagaacg cgcaccccac cagcaaccaa
     4801 cgcttttgcc atcggcactg cgtgttccag tttttttacc acgataaccg gtacaaccgg
     4861 gccggtggtc aggattgatt ctgcacttgt tttccagttt ttcatcagag ttttctctcg
     4921 cctgattaca aatttgtcgt cttaaaaagt gatacaggtt gcgccctgtt cggcaccgga
     4981 cagtttttca cgcaaggcgc tgaataattc acgtcctgtc cccacgcgtg acgcgctcag
     5041 gtcaggaatg tgcggttcgc gagcagccag ttccgcttcg tctaccagca gcgtcagttc
     5101 gcctgtctgt ccattcacac gaatgatgtc cccgtcgcgc acttttgcca gcagcccgcc
     5161 atcgtaggct tctggtgtta cgtggatagc tgacggcact ttacctgaag cgccggagag
     5221 tcgtccatcg gtaactaacg caattttgaa acaccggtcc aataatacac caagtggcgg
     5281 catgagttta tgtaattctg gcattccgtt cgcttttggc ccctgatgac ggacaacaac
     5341 gacacaatcg cggtccagca aacccgcttc aaaggccggc ataacgtcat gctggctttc
     5401 aaaaacaacc gctggcgctt caatcacctg gttctcaacc ggcacggcag aggttttcat
     5461 aaccgcacgg cccaggttac cgcttaacac ttttgtccca ccatgatgag agaaaggttg
     5521 ttcgaaggaa gcgatcacat tgctgtcgag tgatttttcc gccccttccc gccagtccag
     5581 ttcaccatta ttcagccatg gttcaagggt ataacgagac agaccaaagc ctgccaccgt
     5641 attgacatct tcatgcagca ggcctgcttt gagcagttca cgcaccagaa ccggtacgcc
     5701 acctgccgcc tggaagtggt taatatcggc cggaccgttc gggtagagac gtgccatcag
     5761 cggtacaaca tcagaaaggt cagagaagtc atcccagtta atctgaatac cggccgcgcg
     5821 cgccatcgcc accaggtgca tggtgtggtt agtggaacca ccggtcgcca gcagtgcaac
     5881 gataccgttc accaccactt tctcatcgat catcttaccg atcggcatcc attcattacc
     5941 attaccggtc atgcgtgtaa cctgacgcgc agcggcagcg gtcaaagcat cgcgcagcgg
     6001 agaatccgga tgaacaaaag aagagcctgg cgactgcatc cccataaatt ccaccaccat
     6061 ctggttggtg ttggcagtac cgtagaaagt acatgttccc ggcgcatggt aagacgcggc
     6121 ttctgactcc agtaaggcca tgcggtccac tttaccttcg gcataaagct ggcgaatacg
     6181 cactttttct ttatttggca aaccgcttgc catcggtcca gacggcacaa acaccgcagg
     6241 caaatgacca aacgacaggg ctgccatcgt cagacccggg acaatcttgt cgcacacacc
     6301 gaggaacaga gcaccatcaa acatgttatg ggacagcccc accgccgcag acatcgctat
     6361 cacttcgcgg cttagcagcg acaattccat tccatcctgc ccctgggtga caccatcaca
     6421 catcgccgga acaccgcccg caacctgacc aaccgcattc gcttcatgca gggctttacg
     6481 aatgatttct ggatagtgtt cataaggctg gtgcgcggag agcatgtcgt tataggaggt
     6541 gatgatggcg atattgttac gcaacatgct tttcaaagag gctttgtctt ctggctggca
     6601 ggcagcgaaa ccgtgtgcca ggttaccgca tgccaactgc gaacgatgaa cggtcgacgt
     6661 tttcgcttgt tctatccggg cgagataagc agagcgagtc tcgcgcgaac gttcaatgat
     6721 tcgatttgtt acgcgtaaca attgtggatt cataaaggct cctgaaattg agttgtcaga
     6781 gcaggatgat tcacaacgcg tttcattcag aggatttatg agtgaaacgc ctgtaaccgg
     6841 agctcatagg gcaaaaacgt ttcagtcagt gtaataaaaa aagcctcgtg ggtgaatccg
     6901 cacgaggcct gaaagtgtaa aaattgttct acaatctgtg caagatcatg ttaccggtaa
     6961 aataaccata aaggataagc gcagatatta ctcaaactca ttccaggaac gaccatcacg
     7021 ggtaatcatc gccaccgagg caacgggtcc ccaggttccg gcctgatacg gtttcggcgc
     7081 atcattgtcc atcgcccacg cctcagtaat ggagtctacc catttccagg cttcttccac
     7141 ttcatcacga cgtacaaaca gtgcctgaat accacgcatg gtttccagca gcagacgttc
     7201 ataggcatcc gccagatgcg tctgattaaa ggtttctgaa tagctcagat ccagcttggt
     7261 gatttgcagg ttatgtttgt ggtcaaggcc aggaacttta ttcagtacct ggatatccac
     7321 gccttcatca ggttgcagac ggatagtcag tttattctgc ggcagatcct gccacgactc
     7381 tttaaacaga ttcagttcag gtgttttgaa atagaccacg acttcagaac atttggtcgg
     7441 cagacgttta ccagtacgca ggtagaatgg cacaccggcc cagcgccagt tatcaatgtc
     7501 gacgcggatc gccacgaagg tttctgtatt gctgctcttg ttcgcaccct cttcttccag
     7561 atatcccggc acttttttgc cctgggcgaa gcccgcagta tattgcccac gtacggtttt
     7621 ttcgcgtacg ttggagcggt cgatgcggcg cagggacttc agtactttca ctttttcatc
     7681 gcggatgctg tctgcgctca ggtcagatgg cggagacatc gcaatcatgc agagaatttg
     7741 tagcaggtgg ttctgaatca tgtcacgcat ctgaccggct ttatcaaaat agccccagcg
     7801 cccttcgatc cccacttctt ctgccacggt aatctcaaca tgatcaatgg tgcgattgtc
     7861 ccagttattc acaaacaggg agttagcaaa acgcagcgcc aacaggttca gcaccgtttc
     7921 tttaccaaga tagtggtcga tacggtaaac ctggcactcc tcgaagtatt cgccaacctg
     7981 atcattgatt tcctgcgagg tcgccagcga cgtccccagc ggtttctcca tgactacgcg
     8041 tgccggttta gcattcagtt ttgcctcgcc aagccctttg caaattgcgc caaaagtgct
     8101 gggcggcatg gcaaagtagt taatggtgat acgatttttt tgatccagca tcgcgccgag
     8161 acggttgaat gcagcagtgt cattgacatc gagattacaa aaatccagac gtgcactcag
     8221 ggtgtcccat aaaccttcat caatggtttc tttcatgaaa gtttcgagcg cctcgcggac
     8281 aactttggta tatgccgctt tatcccagtc agcacgccct acgccgataa tccgggtgtc
     8341 cgggttgagc tgaccggctt tttccagttg atacagggaa ggcagcaatt tacgacgcgc
     8401 aaggtcgcct ttcgcgccga aaatgaccag gtcacaggcc tgggctgttt gcgttaccgc
     8461 catgtcattc tccttaagtt aactaacccg gtacttaagc cagggtatac ttgtaatttt
     8521 cttacggtgc actgtactgc ttttacgagc ttgcgaaaac tgtaaacgct tatccacccg
     8581 tgcgattacg ggaaaagcgc gcaaagcgcg gcaaaactga taaaaaaatc atcgttttcc
     8641 attcggtaaa aatctgacac tgatcatgtt atgaaaaaaa ataacaactt ttttatctgc
     8701 ttttgtcatt aacacggcac acaggcgtaa tatctgacaa aactgcattt cgatttcttt
     8761 cagtgcggaa atcgtcatta cccgtgagtc tctttacatc atgaatatgc tggaaaaaat
     8821 ccagtctcag ctggaacatt tgagcaaatc agagcgcaaa gttgccgagg tcattctggc
     8881 ttcgcccgat aacgcgatcc attcgagtat tgctgctatg gcactggaag ccaatgttag
     8941 cgaaccgacg gtgaatcgtt tctgtcgcag catggacacg cgcggttttc ctgattttaa
     9001 acttcatctg
//
LOCUS       DADLFA010000164         8898 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000164 DADLFA010000000
VERSION     DADLFA010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8898
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.166"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..233)
                     /locus_tag="JQH65_003302"
     CDS             complement(<1..233)
                     /locus_tag="JQH65_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY6140613.1"
                     /translation="MSRKNQRYSKEFKAEAVRTVLENQLSISEGASRLSLPEGTLGQW
                     VTAARKGLGTPGSRTVAELESEILQLRKALNEAR"
     gene            307..483
                     /locus_tag="JQH65_003303"
     CDS             307..483
                     /locus_tag="JQH65_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HAY6140614.1"
                     /translation="MKRLNKLRGWRKVSRSAILREAVEQYLERQQFPVRKAKGGRQKG
                     EVVGVDDQCKEHKE"
     gene            702..944
                     /locus_tag="JQH65_003304"
     CDS             702..944
                     /locus_tag="JQH65_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6140615.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(1274..2203)
                     /gene="fdhE"
                     /locus_tag="JQH65_003305"
     CDS             complement(1274..2203)
                     /gene="fdhE"
                     /locus_tag="JQH65_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HAY6140616.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLDDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(2200..2835)
                     /gene="fdoI"
                     /locus_tag="JQH65_003306"
     CDS             complement(2200..2835)
                     /gene="fdoI"
                     /locus_tag="JQH65_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HAY6140617.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2832..3734)
                     /gene="fdxH"
                     /locus_tag="JQH65_003307"
     CDS             complement(2832..3734)
                     /gene="fdxH"
                     /locus_tag="JQH65_003307"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HAY6140618.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3747..6797)
                     /gene="fdnG"
                     /locus_tag="JQH65_003308"
     CDS             complement(3747..6797)
                     /gene="fdnG"
                     /locus_tag="JQH65_003308"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(6210..6212),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HAY6140619.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVANYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6991..7824
                     /gene="fdhD"
                     /locus_tag="JQH65_003309"
     CDS             6991..7824
                     /gene="fdhD"
                     /locus_tag="JQH65_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HAY6140620.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            7978..>8898
                     /locus_tag="JQH65_003310"
     CDS             7978..>8898
                     /locus_tag="JQH65_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HAY6140621.1"
                     /translation="MKRNLLSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAP
                     EAPVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQRSLARYVDWLKDFK
                     QGPTGEERTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNT
                     INEMDKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKRQLAEL
                     KNIEEREGKIFHYYSLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADK
                     GGMNFSFINSAGQYQLEAKKYVRRIRDKVPY"
BASE COUNT         2119 a         2249 c         2343 g         2187 t
ORIGIN      
        1 cgagcttcat ttaacgcctt acgcagttgc agaatttcag attccagttc agccaccgtg
       61 cgggaaccag gagtaccgag cccttttctg gcggcggtaa cccattgtcc taaagtgcct
      121 tcaggaaggg ataatcggga agcgccttca ctgatcgaaa gttgattttc aagaaccgtt
      181 ctgacagctt cggctttgaa ctctttagag taacgttggt tttttctgct cattattagc
      241 tccttctgat gccattctat ttcaggaagg agtgtccgtt aaactcaggc tacctcagaa
      301 gaggcaatta aacggctgaa caagcttcgg ggctggcgta aggtttcacg atctgcaatt
      361 ttacgcgaag cggttgagca atatctggag cggcagcaat ttccggtgcg taaagcaaag
      421 ggaggcaggc aaaaggggga ggtagttggt gttgatgatc agtgtaagga gcataaggaa
      481 tgaaaaaaat ttttcattct tgaatataaa aaaacagatg cccttattct ggtattaata
      541 caaggctgtt ttacttgaac ttataataac tgcaactgtt acatcgtatc tggaaaacgc
      601 ctcgcaaaaa taaaaaatga tgcgtaaatg agctttcttt aaagattcgg caagggtact
      661 tttctctcca tagattcaaa aatgatattg gcgagatatt tatgaactct ctggcaggta
      721 tagatatggg cagaatttta ctcgatttat cgaatgaggt gattaagcaa cttgatgatc
      781 tcgaggtgca gcgtaatctt cctcgtgcgg acctattaag ggaagcggta gatcaatacc
      841 tgataaatca atcgcaaaca gcaagaacca gtgttcctgg catctggcaa gggtgtgagg
      901 aagatggtgt cgaatatcag cgtaagctgc gcgaggaatg gtaatgggaa aaaatgatgt
      961 caatcagatt gctgacaacg tgcgcgttgt tcatgccgga tgcggcgtga acgccttatc
     1021 cggcctacaa agtcatgcaa attcaatata ttgaagttta ttggtaggcc tgataagcgc
     1081 agcgcatcag gcaatcatac gcttgtcatc agtgacatac ccataacctt atcgatgtgt
     1141 tggagggaag aacgaggcaa acagacaatt gcggcatgat cttgaatggg taacgcggaa
     1201 tacgagagat ggtgtcgtca caccctatga gctgtaatcc gggcgaccag cgccacccgg
     1261 aaaacgtcag agattactcc ccttcacccg gaaacaggaa cgggttgatg gaactgcggg
     1321 catagccttc ttgctccatt cgcgcgtcca gtaccagaga agcgaggtca tctgccacgg
     1381 cttcaacttt cggttctttt tcctgataaa gaatcttcag gtaagtgccg cagtcatcgc
     1441 agctttcggc tttaatcgcg gcctgttcgt catccagcga ccagtaatgc agtttgccgc
     1501 tctgttcaca gttgctgcat tttacgcgca ctacgtgcca ttcggtttca cacaggttgc
     1561 agtgcaggta acgcagaccc tgagtggtgc caatttgcac catgctggac accggcatag
     1621 aaccacaaac cgggcaatat tgacgttgct cgccgtattc agcgcgggct ttgccgggga
     1681 tcagattggc catctgcgcc cagtagagcg acagtgcagc ccagataaac ggcgctttat
     1741 cgctgctgac ggatgagaaa tcagaggcaa acagtgcgct ggccatatct tccagctcct
     1801 gagtcgatgc cttctccaga ttctcaatca ctgccagcgc cgggccgctc atttcaggtt
     1861 tcagctcagc aatcagcgcc atcagcagct tttgccagtg cttatcacgc ggcagaacgt
     1921 gaatatccag cgggggcttg ccctgtgcgc tggcttcttt aatgcgcgcg gtcagatcca
     1981 tctccagcgg atggtcgtac agcaccactt cctgggcgtg ggcgataagc gcagcaaagc
     2041 gcaggtaatc atccagcgga ttattttctg ccagctcgcg cagacgctcg gcgcggcggt
     2101 tgtataaatt cttgagccga gggaacaata acggcggaat catatccgcc gtacgtttct
     2161 cgctcgaacc cagctcatct tgcgggatta tgcgaatact cattcagctt tcttttccgt
     2221 tgtcttgcgg acctcacggt accagcgcgg gtgatgtttc ttcgcccagg cgctggtaac
     2281 ccatccttcc accatggcgg taatcgtgcc tttcacccaa agggcggcgt agatatgcac
     2341 catgataacc acaattaacg ccactgcggc aaatgaatgc agcattaacg cgaatcggat
     2401 caccgggatt gagaaagcag gcgcaaaata aggacgccag ataatcacgc cgctcaccag
     2461 cagcagaacc aggaaaataa tcgccgccca gaaaacgcat ttctgaccga agttataacg
     2521 cccggtgtca cctacttcct cgttgacgac gatcttacga atattcttcg cccaaaagat
     2581 atcatcccga ttgattaggt tgtgatgcca gtaacggaaa aacatgatga tgaacgaggc
     2641 aaacataacc acgccgacaa acgggtgcag aattcgcgcc agctgcggtg tgcccatgat
     2701 ttgcatcaac cagttgaagg acgggaacaa aaagcccagc ccgctcaccg ccgctaggat
     2761 gaagcagaag gcggtgatcc agtggttgat acgttccggc gcggtgtagc gcacgatggt
     2821 gtcacgtcgt ttcatttgcg ctcctcgtct ttctcttcgt gcagattatt ctcttcctca
     2881 tccgcacggt tcggaccgac acccacgtag tggaagatac tggctgcgaa ggtagccgca
     2941 aagccaacag ctgcgagcgg tttccagatg cctttccaga atttcacggt ttcgctgatt
     3001 tccgggttct ccggcaagcc atgatacaga tttggcttgt cagcatggtg cagcacgtac
     3061 ataacgtgtg taccaccgac gcctgccgga tcgtacaggc ccgcattgtc gtaaccgcgg
     3121 gttttcagct ccgccacgcg ctcgctcgcc agcgttttca tcgactcttt cgtaccgaag
     3181 tgaatcgcgc ccgttgggca ggtcttcacg caggctggtt cttgcccaac caccacgcgg
     3241 tcaacgcaca gcgtacattt gtagacgcgg ttgtcttccg ggttgaggcg cggaatgtcg
     3301 aacggacagc ccgcaatgca ataaccgcag ccaatgcact gctcggactg gaagtcgacg
     3361 ataccgttgg catactgaat aattgcacct tccgccgggc acgctttcag acagcctgga
     3421 tcggaacagt gcatacagcc gtctttgcgg atcagccatt ccagtttgtc gttctgctcc
     3481 acttccgaga agcgcatcac cgtccacgat ttggcactta aatcattggg gttgtcgtac
     3541 accccaatgt tattgccgac ggtatcgcga atgtcgttcc actctgaaca cgccacctga
     3601 caggctttac agccgataca ggtggtgacg tcgatgagtt tcgccacttc ttcctggaag
     3661 tcccgcgcct gaggcgcggg ggtcagaccg ttagtcgcgg aacgacgaat gatatcttga
     3721 gattgataag ccatatgtcg tctccgttac accttttcca cattcacaag gaaggactta
     3781 aactccggcg tctgcgtgtt cgcatcacca acgaatggcg ttaaagtatt ggcaataaag
     3841 ccttttttag ctacgccttc atagccccag tgaatcggaa taccgatggt atcgatatct
     3901 ttgccgttcg ctttcagcgt gcgaatacgt ttggtcacca ccgctttggc tttgatatag
     3961 ccacggttgg aggagacttt cacggtatcg ccctgggcaa tgccaagttt attcgccagc
     4021 gactccccga tttccacaaa ctgctctggc tgcaaaatcg cgttcaacag cgcgtgtttg
     4081 gtccagtagt ggaagtgctc ggtcagacga taggtggttc cgacatacgg gaacttatcg
     4141 gctttaccca atgcttcggc gtcgtcttta aagatacgtg cagccgggtt cgagataacg
     4201 tttggatgca gcgggttagt tcccagcggc gtttcaaacg gctcgtagtg ttccgggaac
     4261 ggaccttccg ccatcttatc gagggcaaac agacgcccca tgccttcctg ctgcatgata
     4321 aacggcccga cgccgctacc cggaggcgct gcgctgtagt ccggaatatc ccagccggtc
     4381 cacttagtgc cgtcccattt cagcaactga cgcttcggat cccacgggtt accctgcgga
     4441 tctgcggagg cgcggttata cagaatgcgg cggttaagcg gccatgccca tgcccagccc
     4501 agcgtgttac cgaggccaga cggatcggcg ttatcacgac gcgccatctg gttgccttcc
     4561 ggcgtccagc taccggcgaa aatccagcag ccacaggagg ttgtaccgtc atcgcgcaat
     4621 tgggcgaacg agctaagttg ttggcctttc ttgacgataa ccgcaccggt tgccggatcg
     4681 gtaatatcgg ccagcgcctt accgttgctc tccatcgcta cttcttccga cttcggctca
     4741 tgcggaatgg cgtagttcca ggtcatgttc agcacctgat ccgggttcgc gccaccctgt
     4801 tcggcataca tcttgcgcaa gcgcaggaag ataccggaga ggatctcacc gtcagtcagc
     4861 gcaatccccg gggcgtccgc acctttccag tgccactgca accagcgacc tgagttaacg
     4921 attgaaccgt tctcttctgc gaagcaggtc gatggcagac ggaacacttc ggtctggatc
     4981 ttcgacgagt caacttcgtt cagctcaccg tggttctgcc agaagttaga ggtttcagtg
     5041 ttcagcgggt cgatggtgac gaggaacttc agtttcgaca gacaaccgat cactttgttt
     5101 ttgttcggga atgaggcaac agggttaaaa ccctggcaga tatagccatt gaccttgccc
     5161 tctttcatca tctcgaagta ttgcaggacg tcgtagcctt tatcccactt cggcaaccag
     5221 tcaaagcccc agctattttc cgccgtcgct ttatcaccaa agaaggcctt catcatagag
     5281 acgaagaatt tcgggtagtt gccccagtag tttacctggc cttccagcag cggttttggc
     5341 gtgttggcgg taaggtaggt ttgcagatcg gtctgcttct cgcttggcag cgtcatgtaa
     5401 cctggcaggc tctgcgacag cagccccagg tccgtcagcc cctgaatatt ggagtgaccg
     5461 cgcagggcgt taacgccgcc gcctgccatc cccatattgc cgagcagcag ctggatcatc
     5521 gccatcgtac gaatgttttg cgcaccaacg gagtgttgcg tccagccgag ggcatacagg
     5581 aacgaggcag ttttatcgtg agcactggtt tctgcgatgt attcgcagac tttcaggaac
     5641 gcgtcttttg gcgtaccaca gatgttttca accacatctg gcgtgtagcg ggaaacgtgc
     5701 tgtttcagca agttccacac gcagcgcgga tgttgcagcg tggtatcgcg tttggcgaag
     5761 ccgttttcgt ccagttcgta agtccaggag gatttatcgt acttgcgttt ttccgcgtca
     5821 tagccggtga aaaggccatc ttcaaagccg taatcctcac gcacgatcag gctggcgttg
     5881 gtataggctt cggtgtattc gcggttgaat ttttcattgt tcagcaggta cagcaatacg
     5941 cctgacagga aagcaatgtc agtaccggaa cgaatagggg catagtagtt agctaccgcc
     6001 gccgtacgcg taaagcgagg atcgatcaca atcagcttcg cgccgttgtg aattttggct
     6061 tccatcgccc agcggaaccc gaccgggtga gcttcagcgg cgttaccgcc catcaccacg
     6121 acgaggttgg cgttcttgat gtcgacccag tggttggtca tcgcaccgcg accaaatgtt
     6181 ggagcaagac ttgctaccgt tggtccgtgt cagacacgcg cctggttgtc gaccgcgagc
     6241 atacccagcg cgcgggagaa tttttgcgtt aaatagccgg tttcgttact cgacgcggaa
     6301 gcacacagca tcccggtgga gagccagcgg ttaacagtca cgccttcggc gttttgcgca
     6361 atgtagttag catcgcggtc ttctttcatc agtttggcga tgcgatcaaa cgcctcttcc
     6421 caactgattt gctgccattt atcagaacct ggcgcacggt attccggaaa cttcagacgg
     6481 ctttcggagt ggatgaaatc caccaggcca gcgcctttcg gacaaagtgc accgcggttg
     6541 accgggtgat ccggatcgcc ttcgatatgg aagatagatg ctttggcgtt ttttgctccg
     6601 tcaccgaggc tgtacatcaa cagcccacag cctacggaac aataggtgca ggtattacgg
     6661 gtttcgcggg tgcgcagcag tttatactgc cgggtttccg cgagtgctac gctgggtgca
     6721 aaacctagtg ccgctgccgt ggtgcctgcc ataccgccag cgcagatctt aaagaactgc
     6781 cttctgctga cctgcatgga ttgctccttg tttcgacatt gtcacgtccc atttacattc
     6841 gcttgctgcg tgtgcaggga gtgggagtta tttttctttg cggaaggggc cgcaaaggtc
     6901 cagaattggc tcgatttccc tccatccagg aagggtttgt aacagaatac cataatgttg
     6961 gtgtgtgtgt tcttatctgg ttaagagaaa gtgaaaaaaa cacagcgaaa agaaatcgaa
     7021 aatgtgacaa atatcacagg tgttcgtcaa attgagttat ggcggcgtga tgatttacaa
     7081 cacccacggt tagatgaggt cgcggaagaa gttcccgttg cgctggtcta caacggcatt
     7141 tcgcatgtgg tgatgatggc gtcgcccaaa gaccttgagt actttgcgct cggtttttcg
     7201 ctttccgagg ggattatcga aagtccgcgc gatatcttcg gcatggatgt cgttccttcc
     7261 tgtaatggtc ttgaagtaca aattgagctt tccagccgcc gctttatggg gttgaaggag
     7321 cgccgccggg cgctggcggg acgtacgggc tgtggcgtat gcggcgtgga gcaacttaat
     7381 gacatcggaa aaccggtgca gccgctaccg ttcacccaga cgtttgatct caacaaactg
     7441 gatgatgcat tacgtcatct caacgatttt cagccagtgg ggcaactgac tggttgtact
     7501 cacgccgctg cctggatgtt gccatctggc gaactggtcg gcgggcatga agacgtgggg
     7561 cgccatgtgg cgctggacaa actgttaggc cgccggtcac aagaagggga aagctggcag
     7621 caaggtgcgg tactggtttc cagtcgtgcc agttatgaaa tggtgcaaaa gtcggcgatg
     7681 tgcggcgtag agattttgtt tgcggtgtct gccgcgacca cgcttgctgt agaagtggct
     7741 gagcgctgta atctgacact ggtaggtttt tgtaaaccgg gtcgggcaac ggtttatacc
     7801 catccgcagc gtttgagcaa ttaattaaaa taagtcctgc gatatatatt aaattgcagg
     7861 acttattcat ttcgtgaatt ttattatttt tatttataaa taaaacgcgt gtagtggcgt
     7921 tttctttttt catttattat gttcactctc ttatctatat atttataagg caattaaatg
     7981 aaaagaaatt tattatcttc cgctattata gtcgccatca tggccctcgg tctgacgggt
     8041 tgtgatgata aaaaagccga aacagaaacg cccccgcctg ccaatagtca acctgccgca
     8101 ccagctcctg aagctcccgt tgcaaaagca gaagctaaac ctgaaacacc tgcgcaaccg
     8161 gtggtcgatg aacaagcggt tttcgacgaa aaaatggatg tctatatcaa gtgctataac
     8221 aagttacaga tccctgtaca gcgcagtctg gcgcgttatg ttgactggct gaaagatttt
     8281 aaacaggggc ctaccggtga agagcgtact gtttatggca tctacggcat tagtgaatcc
     8341 aacctcgctg agtgtgaaaa aggcgtaaaa agtgctgtgg cgttaacgcc tgcgctgcaa
     8401 ccaattgatg gcgtggcggt gagttatatc gatgctgccg tggcgttggg taataccatt
     8461 aacgaaatgg ataaatatta cacccaggaa aattataaag acgatgcgtt tgcgaaaggg
     8521 aaaacgctgc accagacatt cttaaaaaat ctggaagcct ttgaacctgt agcagaatct
     8581 tatcatgcgg cgattcagga aattaacgat aagcgccagc ttgcggaact gaaaaatatt
     8641 gaagaaagag aagggaaaat attccactac tattctctgg cagtcatgat ttcagcgaaa
     8701 caaattaata acctgatatc gcaagagaag tttgatgttg atgcggcaat gaagaaagtg
     8761 tctgaactgg aaacgctggt ggcgcaggcc aaagaagcgg ataaaggcgg catgaatttc
     8821 tcgtttatta attcggcagg ccagtatcaa ctcgaggcta aaaaatacgt tcgccgcatc
     8881 agagataaag tcccgtac
//
LOCUS       DADLFA010000165         8736 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000165 DADLFA010000000
VERSION     DADLFA010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8736
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.167"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            123..743
                     /locus_tag="JQH65_003311"
     CDS             123..743
                     /locus_tag="JQH65_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAY6140622.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            1052..2464
                     /gene="cycA"
                     /locus_tag="JQH65_003312"
                     /gene_synonym="dagA"
     CDS             1052..2464
                     /gene="cycA"
                     /locus_tag="JQH65_003312"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HAY6140623.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFLG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(2509..3171)
                     /gene="ytfE"
                     /locus_tag="JQH65_003313"
     CDS             complement(2509..3171)
                     /gene="ytfE"
                     /locus_tag="JQH65_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HAY6140624.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYNMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(3279..4244)
                     /locus_tag="JQH65_003314"
     CDS             complement(3279..4244)
                     /locus_tag="JQH65_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY6140625.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLIRQQMPPLMTLSGIALLVVGVVIVVRAKPEKPLTESVSES"
     gene            complement(4352..5212)
                     /gene="qorB"
                     /locus_tag="JQH65_003315"
     CDS             complement(4352..5212)
                     /gene="qorB"
                     /locus_tag="JQH65_003315"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HAY6140626.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            5301..5681
                     /locus_tag="JQH65_003316"
     CDS             5301..5681
                     /locus_tag="JQH65_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAY6140627.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(5810..7753)
                     /gene="cpdB"
                     /locus_tag="JQH65_003317"
     CDS             complement(5810..7753)
                     /gene="cpdB"
                     /locus_tag="JQH65_003317"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HAY6140628.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEEADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDRHIAFASPDE
                     NRSVLTAWIADESKRAGEIHPAADNNWRLAPITGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            7943..8683
                     /gene="cysQ"
                     /locus_tag="JQH65_003318"
     CDS             7943..8683
                     /gene="cysQ"
                     /locus_tag="JQH65_003318"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HAY6140629.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
BASE COUNT         2012 a         2220 c         2291 g         2213 t
ORIGIN      
        1 cgggatattg ctattgtttc tttttccccg ggatttgtca tcattcctgt gacaaagttt
       61 tacccaaaga agtgtggctg atatgctgcc cgctactttt ataccctaag aaaggaaata
      121 cgatgaccac cccaactttt gacaccatcg aagcgcaagc aagctacggc attggtttgc
      181 aggtagggca acaactgagt gaatctggcc tggaagggct gctgccagaa gcactggttg
      241 caggtattgc cgatgcgctg gaaggcaaac atccggctgt tccggttgat gtggtgcatc
      301 gcgcgctgcg tgaaatccac gaacgcgccg atgccgttcg tcgtcagcgt ttccaggcga
      361 tggctgctga aggtgtgaaa tacctggaag aaaacgccaa aaaagaaggt gtgaatagca
      421 ccgaatctgg cctgcaattc cgcgtgatca accagggtga aggcgcaatt ccggcacgta
      481 ccgaccgcgt tcgtgttcat tacaccggta aactgatcga cggcaccgtg tttgacagct
      541 ccgttgctcg tggtgaaccc gctgaattcc cggttaacgg cgtgatccct ggctggattg
      601 aagcactgac tctgatgccg gtaggttcta aatgggaact gactatcccg caggaactgg
      661 catatggcga gcgcggcgca ggtgcatcca tccctccgtt cagcaccctg gtgtttgaag
      721 tcgaactgct ggaaatcctc taagcagcgc attctgttcc cctcgaacca gaggggagca
      781 ggcattcagc aataaaccct tcagtttgcc aaacggcgct attttgtgtt gcaaagaccc
      841 cgtaagcgtg tatttttgtg agatgtttcg cgttatcacc gtgatatgac actcacttta
      901 aacataaaat taacattaga tctaaatctt agtattcatc ccgcgtattg ttacctaata
      961 tcgatgagtc ccgatacaga ttcgtcgtat catagactga ctaaaggccg tagagcctga
     1021 acaacacaga caggtacagg aagaaaaaaa catggtagat caggtaaaag tcgttgccga
     1081 tgatcaggct ccggctgaac agtcgctacg gcgcaatctc acaaaccgac atattcagct
     1141 tattgccatt ggcggtgcca ttggtacggg gttgtttctg gggtctggca aaacgattag
     1201 ccttgccggg ccgtcgatca ttttcgttta tatgatcatt ggttttatgc tctttttcgt
     1261 gatgcgggca atgggggaat tgctgctttc gaatctggaa tacaaatctt ttagtgactt
     1321 cgcttccgat ttactcgggc cgtgggcagg atatttcact ggctggactt actggttctg
     1381 ctgggttgta accggtatgg cagacgtggt ggcgatcacg gcttatgctc agttctggtt
     1441 ccccgatctc tccgactggg tcgcctcgct ggcggtgata gtgctgctgc tgacgctcaa
     1501 ccttgccacc gtgaaaatgt tcggtgagat ggagttctgg tttgcgatga tcaaaatcgt
     1561 cgccatcgtg tcgctgattg tcgtcggcct ggtcatggtg gcgatgcact ttcagtcacc
     1621 gactggtgtg gaagcgtcat tcgcgcattt gtggaatgac ggcggctggt tcccgaaagg
     1681 tttaagtggc ttctttgccg gattccagat agcggttttc gctttcgtgg ggattgagct
     1741 ggtaggtaca acagctgcgg aaaccaaaga tccggagaaa tcactgccac gcgcgattaa
     1801 ctccattccg atccgtatca ttatgttcta cgtcttcgcg ctgattgtga ttatgtccgt
     1861 gacaccgtgg agttcggtag tcccggagaa aagcccgttc gttgagctgt ttgtgttggt
     1921 aggtttgcct gcggctgcca gcgtgatcaa ctttgtggtg ctgacctctg cggcgtcttc
     1981 cgctaacagc ggcgtcttct ctaccagccg tatgctgttt ggtctggcgc aggaaggtgt
     2041 ggcaccgaaa gcgttcgcta aactttctaa gcgcgcagta ccagcgaaag ggctgacctt
     2101 ctcctgcatc tgcctgctcg gtggcgtggt gatgctgtat gtgaatccta gcgtgattgg
     2161 tgcgttcacg atgattacaa ccgtttccgc gattctgttt atgttcgtct ggacgattat
     2221 cctttgctcg tacctggtgt accgcaaaca gcgtcctcat ctgcatgaga agtcgatcta
     2281 caagatgccg ctcggcaagc tgatgtgctg ggtatgtatg gcgttctttg tgttcgtggt
     2341 cgtgttgctg acactggaag atgacactcg ccaggcgctg ctggttaccc cgctgtggtt
     2401 tatcgcactg gggctgggct ggttgtttat tggtaagaag cgggctgctg aactgcggaa
     2461 ataaccgcat tatcatgctg gatggcgcaa tgccatccag cttttagatc actcacccgc
     2521 cagcgcgcgt gggaacagta cattgttttc cagactgatg tgatccatca ggtcatcaat
     2581 cagttcatta atgccgttat acatcgcttt ccaggtggtg caggcttctg gcggcggtgt
     2641 gacgttattg gtagtgtgtt tgatcacttc cagcagttcg cccgcttcat cgtgctcgct
     2701 ttccattacg ctgattggcc ccattgcctg gctgcccatg ccttgtttga tcatcgggaa
     2761 gaggatctgc tcttctttca tcatgtggct ggaaagctct tcatgcagca tggtcaggta
     2821 ttttgtcagc ccttttggca cgctcggttt gtcggcgtga acgcgctcga ctttagtcgc
     2881 ttgcaggatc agctccggca gttgctcgcg gtgacgatcg tggtagcgca cgatgatatg
     2941 gtcaatgatt tctgccagcg gggcgctacg ccagtctttc tcaatcggtt gttcagcaag
     3001 ctttgccagt tcagcttcaa tgacctcaac atccagttct ttacgtgccg ccgcgcgcgc
     3061 cagcgtctgc ttaccgccac agcagtaatc catattatat ttacgaaaca gagctgaagc
     3121 gcgaggaata gagagcgcca gttcacctaa aggttggtcg cgataagcca tagctgatac
     3181 ctcattctta ataagataag atgtatttta aatacatctt taaggcaaaa gctataaccc
     3241 ttacgtagca gaggcttatt taactcacag ccagtatgtc aactttctga gacagattca
     3301 gttaaaggct tttccggttt tgctctgact acaatgacca cgccaaccac taacagcgcg
     3361 ataccgctca gcgtcatcag cggcggcatt tgctggcgta tcaaaaaggt gtacagcaaa
     3421 cctgccagtg tttcgaaaac gatcagcggc ccgagaatca ctgtcggtaa tcgctggctg
     3481 gcgacgttcc agcagagtgc gccaacccat gagcaaagca cggctatcgc aaccatcaga
     3541 ctaataaaca ccagcggacg ggggccaaaa ggtagggaga agtccggcgt ttgcgtattc
     3601 agccagtaac aggcgacgag atagccgatg agagaaaccg gcagtgtgac cagcgcctgc
     3661 gccgtcgccc acatcatcgg atgtttgtcg ggattctccc gcagccagcg ggcgttgcgc
     3721 agggcatacc atgcccagca gaccacggaa actaacgcta gcacgatgcc tgaggtataa
     3781 cgtgcccagt caaaatcggg gagtccgtgg tttaactcag caatattcac acacgccagg
     3841 ccgatgccaa tacaaaccag tgccggggcg agttttcccc acgcgagttt gccgtcgcgc
     3901 tggctataaa gcagattggc aaaaacggga atcaccaccg gcagggtgcc gataatcatc
     3961 gtggaaacag gcgcaccagt acgttgaatg gcactggcaa ggcagaagta atagatgagg
     4021 ttgcccatca tagtgagcat caaggcggta agccagtcgc gacgcgccaa ctgacgcaga
     4081 cgcacgcgtc ccagccaggc aatgggcagc gcaattaacc ctaacgccag ataacgcccc
     4141 atcgactgca acatcgccgg gtattccggt acgatcaacg gcccgacaaa aataagcccc
     4201 cacatcaacc ctgctaacag ggcgtacagc acgccgctaa tcattactgg catccattgg
     4261 tctgtcagaa gaaagttcag gaagcagaca tgattgctcc cggcatcccg tcattattag
     4321 agaggatgcg ggaggatgcc actttaatta actagttatt aacattaaaa agatggctta
     4381 cgctttcggc taacgttgtc gttgggcggc caatcaattt gctaagcgtt ttgctgttat
     4441 caaacagacc acctttcgat gcgccaacgt cagaatccgc cagcatatcc gccagtccgt
     4501 cgggcagtcc gacgcttttc agtgccgcgg cgaaatcggc ttcgctcaga ttttgatagg
     4561 taacctgttt gccgctctgt ttggtcagct ctgccgctaa ctgtgtcaac gtccaggcac
     4621 tatcgcccgc cagttcgtaa accttgcctt cgtgaccggc ttcgctaatc acgcgtgccg
     4681 cagctgccgc ataatctgcc cgcgttgctg aggcgatttt gccatcgccc gccgcaccga
     4741 taaatacgcc gtgttccagt gctgccgggg cgctggcgag gtagttttcg ctgtaccagc
     4801 cgttgcgcag cagggtgtaa acgatgccag aatcagccaa cattttctcc gtctcaatgt
     4861 gctcatcggc gaggccgagc ggggaggtat ctgcatgtag caggctggta taagcgataa
     4921 atttcacgcc agccgccttt gcggcattaa taacattacg atgctgcggg gcacgttgac
     4981 ccacttcgct ggaagagatc agcagtagtt tttccactcc ctgaagtgca gatgtcagtg
     5041 cggcttcatc gccgtagtca gcctgacgca cggtaatgcc ttgtgctgcc agagcctggg
     5101 cttttgccgg attacgaacg atagccacta tttggctggc aggaaccgtt ttcatcaagg
     5161 attcaataat atagtgacca agttggccag tggcaccagt aatagcgatc atgggaagtc
     5221 tccatcgttt tgtcttgtgt ttaaatacgc taacacctaa acttactttt agtaagtacg
     5281 tacaaaaagg taagtatgaa atgagtcagg ttagcctgtc gcaacaactg aaagagggta
     5341 atctctttgc ggaacagtgc ccgtcgcgcg aggtgttgaa acacgtcacc agccgttggg
     5401 gggtgttgat tctggtggcg ctacgcgaag gtactcatcg ctttagcgac ctgcggcgca
     5461 aaatcggcgg ggtgagcgaa aagatgcttg cgcagtcgtt acaggcgtta gaacaggatg
     5521 gttttcttaa ccgtatcgcg tatccggtgg tgccaccgca tgtggaatat agcctcacgc
     5581 cgctgggcga gcaggtgagc gaaaaggttg ctgcactggc ggactggatt gagttgaatt
     5641 tgcccgaggt gttggcggtg cgggatgaac gtgcggcata actgccggat gcgctgagct
     5701 tatctggtct acggtgcatg gctggtaggg cggataagat gcgccagcat cgcatccgcc
     5761 aataaatgcc ggatgcggtt gataatttat agaccgaaaa agaagtgttt tacttactca
     5821 aatccacctg ataaatcgca aacccgatat catcggtcgc gactttattc atcggatatt
     5881 gccctttctc tttaataaac gctgcggctt tatctgacgg ggaagtttcg aaacggatat
     5941 ccagtttctt atcgccagtt atcggtgcta aacgccagtt gttatctgcc gccgggtgaa
     6001 tttctcccgc acgtttcgac tcatcagcaa tccacgctgt cagcaccgag cggttctcat
     6061 ccggtgaagc aaaagcgata tgtctgtcgc ccgtaccggc aaatttgccg ccgtaagcgc
     6121 gatagttatt ggtggcaacg aggaacatag cgttcggatc aatcggcttg ccattaaagg
     6181 tcaggttctt aatccgctct gcattggcat taatcatctg gcactcgccg tcatagcggg
     6241 cgggttgggt aacatcaatc tgataattca caccatcaat aacatcaaag ttataagtgc
     6301 ggaaaccatc ccagttgatg agtgactgtg gtttcgtgct gttaggatca atctggttaa
     6361 actgtccggc ggagcactcc agccactctt tcacctcttt accgctggct ttcactacaa
     6421 tcagcgtatt ggggtagaga taaagatcgg cggcattacg gaaggtcaac tggccttttt
     6481 ccacctccac atagcttgcc gggtcatttt tgcgaccacc aactttaaac ggtgcggcag
     6541 ctgaaagcac cggcagtttt gccagatccg gatcgccctg aatgtaatgt tcgacatacg
     6601 ctttttgcgc gttgttcacg acttgcacgg tcggatcgtc ctgcaccagc gccagatagc
     6661 tatacatatt gtcggcagat ttaccgattg gcttgctgac gaactggcgt gtggcatcgt
     6721 gatcggcttt gagtgtttct accagcttgc tgtcttccgc cgcgagggat tttttattgg
     6781 cgatgtcgta aatcggccgt gcttccgctt tcgcctgcgt cacctgccat ttaccgctgt
     6841 cattactgag ttgtaagtcg accaccccaa gatgatcgcc ccacatgccc ggcattaccg
     6901 ccggaacacc attcagcgtg cctttggtga tatcagcctc ttcgatatca gcaaaatctt
     6961 tacccgggaa aacggcgtga gcatggccaa acataatggc gttaacgccc ggaatttcac
     7021 tgaggtaata aactgagttt tccgccatta ctttatacgg atcggcagac agcccggaat
     7081 gcgccagaac gacaacgaca tcggcacctt tctcgcgcat ttcaggcacg tatttgcgca
     7141 cggtttcggt aatatcattc accgtcactt ttccggataa attagcttta tcccagccca
     7201 tgatttgcgg cggcacgacg ccaatatagc caatcttcag cgtctgtttt tttccgtctt
     7261 tatcgaccac ttcggtatct ttaattaaat acggtgtaaa cattggctgt ttggttctgg
     7321 cgtcaatgac gttggcattt acataaggga atttcgctcc cgccagcgca tttttcaggt
     7381 aatccagacc gtagttaaat tcatgattgc cgagtgtacc gaccgtataa tccagcgtat
     7441 tcagcgcctt ataaaccgga tgaacatcac ctgcttttaa tcctttcgcc gacatgtaat
     7501 cggccagcgg actcccctga atcaaatcgc cgttatcgac cagtacgctg tttttcactt
     7561 cattgcgggc atcgttaatc aggcttgccg tacgtaccag tccgaatttt tccgtggcgg
     7621 tgtctttgta ataatcgaaa tccatcatgt tgctatgcag atcagtggtt tccatgatac
     7681 gcagatcgac cgtcgctgca ttcacactgg cggcaatcag cgtggccagg agcgttgcgc
     7741 taaacttaat catcagggac atccttttat catcgggaat acgaaagaaa agggagaata
     7801 aacgtcttac ttatagaaca gtgaagaatg ccacaatttt acgctttgaa aatgatgaca
     7861 ctatcacagt tggcgcattc attaacgata gggtataagt aaaacaataa gttaacaccg
     7921 ctcacagaga cgaggtggag aaatgttaga tcaagtatgc cagcttgcac ggaatgcagg
     7981 cgatgccatt atgcaggtct acgacgggac gaaaccgatg gacgtcgtca gcaaagcgga
     8041 caattctccg gtaacggcag cggatattgc cgcacacacc gttatcatgg acggtttacg
     8101 tacgctgaca ccggatattc cggtcctttc tgaagaagat cctcccggtt gggaagtccg
     8161 tcagcactgg cagcgttact ggctggtaga cccgctggat ggtactaaag agtttattaa
     8221 acgcaatggc gaattcaccg ttaacattgc gctcattgac catggcaaac cgattttagg
     8281 tgtggtgtat gcgccggtaa tgaacgtaat gtacagcgcg gcagaaggca aggcctggaa
     8341 agaagagtgc ggtgtgcgca agcagattca ggtccgcgat gcgcgcccgc cgctggtggt
     8401 tatcagccgt tcccatgcag atgcggagct gaaagagtat ctgcaacagc ttggcgaaca
     8461 tcagaccacg tccatcggct cttcgctgaa attctgcctg gtggcggaag gacaggcgca
     8521 gctgtacccg cgcttcggac caacgaatat ttgggacacc gccgctggac atgctgtggc
     8581 cgcagctgcc ggagcgcacg ttcacgactg gcagggtaaa ccgctggatt acactccgcg
     8641 cgagtcgttc ctgaatcctg ggttcagagt gtctatttac taaattcaga tggcagaaac
     8701 agtgtatttc ctgattctgc catcctgatt tctccc
//
LOCUS       DADLFA010000166         8735 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000166 DADLFA010000000
VERSION     DADLFA010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8735
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.168"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            245..1156
                     /gene="ttdA"
                     /locus_tag="JQH65_003319"
     CDS             245..1156
                     /gene="ttdA"
                     /locus_tag="JQH65_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HAY6140630.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            1153..1758
                     /gene="ttdB"
                     /locus_tag="JQH65_003320"
     CDS             1153..1758
                     /gene="ttdB"
                     /locus_tag="JQH65_003320"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HAY6140631.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRGKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            1807..3270
                     /locus_tag="JQH65_003321"
     CDS             1807..3270
                     /locus_tag="JQH65_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HAY6140632.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVFGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(3313..4326)
                     /gene="tsaD"
                     /locus_tag="JQH65_003322"
     CDS             complement(3313..4326)
                     /gene="tsaD"
                     /locus_tag="JQH65_003322"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAY6140633.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRLGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            4563..4778
                     /gene="rpsU"
                     /locus_tag="JQH65_003323"
     CDS             4563..4778
                     /gene="rpsU"
                     /locus_tag="JQH65_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAY6140634.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            4889..6634
                     /gene="dnaG"
                     /locus_tag="JQH65_003324"
     CDS             4889..6634
                     /gene="dnaG"
                     /locus_tag="JQH65_003324"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAY6140635.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            6829..8670
                     /gene="rpoD"
                     /locus_tag="JQH65_003325"
     CDS             6829..8670
                     /gene="rpoD"
                     /locus_tag="JQH65_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAY6140636.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         2111 a         2330 c         2333 g         1961 t
ORIGIN      
        1 acctgcaagt ctttggctaa aggccagcta ttcagcatcg ttaattatcc gcagttgtga
       61 taagcgcagt gtatttcgca aaacattgcc attccctgct ctggcgcaat attcattctt
      121 attttcgcgt tgattcacga actgttaatt acatattcac ggtggcaaaa aatataaaac
      181 cacattttta gtggtagttt atggcggtga atttttccag acaaatacaa aaactggagt
      241 tgccatgatg agcgaaagta ataagcaaca ggcagtgaat aagttgacag agattgtcgc
      301 taactttacc gccatgattt ctacccgaat gcctgatgac gtggtggata aactaaaaca
      361 gctaaaggat gccgaaacgt cgtcgatggg gaaaattatc taccatacga tgttcgacaa
      421 catgcaaaaa gcgattgacc tgaatcgtcc tgcctgtcag gacaccgggg agattatgtt
      481 cttcgttaaa gtcggttccc gcttcccact gcttggcgag ctgcaaagca tactcaaaca
      541 agccgtggaa gaggcaaccg tcaaagcgcc actacgtcac aatgcggtag aaatttttga
      601 cgaagtaaac accggcaaaa ataccggtag cggcgtaccg tgggtcacct gggacatcat
      661 ccccgacaat gacgatgcgg aaatcgaagt ttacatggca ggtggcggct gcacgctacc
      721 tggccgctcg aaagtgttaa tgccgtcaga aggctacgaa ggcgtggtga aattcgtctt
      781 cgaaaatatc tccaccctcg ccgtaaacgc ctgtccaccg gtactggtgg gcgtgggcat
      841 cgccacctcg gtggaaaccg ccgccgtact ctcgcgtaaa gccattttgc gcccgattgg
      901 cagccgccat cccaatccaa aagcggcaga actggagcta cgcctggaag aaggactcaa
      961 ccgtctgggg attggtccac aagggctgac tggcaacagt tcagtgatgg gcgtacatat
     1021 cgaatctgcc gcccgccatc cgtcaaccat cggcgttgct gtctctaccg gctgctgggc
     1081 gcatcgtcgc ggcacactgc tggttcatgc cgatctcacc tttgaaaatc tgtctcacac
     1141 ccggagcgcg ttatgaaaaa gatcctgaca accccgatca aagctgaaga tctgcaagat
     1201 attcgcgtcg gcgatgtgat ctacctgacc ggtacgctgg tgacctgccg tgacgtttgt
     1261 caccgccgct tgatcgaact gaaacgtccg atcccttacg atctcaacgg caaagcgatt
     1321 ttccacgctg gccccatcgt gcgcaaaaac ggcgataaat gggagatggt ttccgttggc
     1381 ccgacaacca gtatgcgtat ggaaagtttt gaacgtgaat ttatcgagca gaccggcgtg
     1441 aaactggtgg ttggcaaagg cggaatgggg ccgctgaccg aagaaggctg ccagaaattc
     1501 aaggcgctac atgtgatttt cccggcaggc tgcgcggtgc tggcggcaac ccaggtggaa
     1561 gagattgaag aagtgcactg gacagagctc ggaatgccgg agtcgctgtg ggtctgccgg
     1621 ggcaaagagt tcggcccgct gattgtctct attgataccc acggcaacaa tctgatagcc
     1681 gaaaacaaaa agctgttcgc cgaacgccgc gatcccatcg tggaagagat ctgcgagcac
     1741 gtccattaca tcaaataacc ctcccggaga ggctcgcccc ctctcctttt tcgcaggcat
     1801 aacacgatga aaccttccac tgaatggtgg cgataccttg cgccgctggc ggtcatcgcc
     1861 attattgctc taattccggt tcccgcaggg ctggagagtc atacctggct ctactttgcc
     1921 gtttttactg gcgtgatcgt tggactgatc ctcgaacccg tgccgggtgc cgtggtggcg
     1981 atggtgggta tctccatcat cgccatactc tctccctggc tgctgttcag ccccgaacag
     2041 ctcgcccagc caggctttaa gttcaccgcc aaatccctct cctgggcagt ttttggtttt
     2101 tctaattcgg ttatctggct cattttcgcc gcctttatgt ttggcacagg ctatgaaaaa
     2161 accgggcttg gacgccgcat tgcgctgatt ctggtgaaaa agatgggaca tcgcacgctg
     2221 tttctcggct atgcggtgat gttctccgag ctgatcctcg cacctgtaac accgtccaac
     2281 tcggcgcgtg gtgcggggat tatctatccc atcatccgta acctgccacc gctctatcaa
     2341 tcacaaccaa acgacagcag ttcgcgcagc attggctcgt acatcatgtg gatggggatt
     2401 gttgccgact gcgtgaccag cgccattttc ttgacggcga tggcaccaaa cttgctgtta
     2461 attggactga tgaaaagcgc atctcacgcc acgctgagtt ggggcgactg gttcctcggg
     2521 atgttgccgc tcagtatttt gctggtgctg ctggttccct ggctggctta tgtgctgtac
     2581 ccgccggtac tgaagtctgg cgatcaggtg ccgcgctggg cagagacgga actgcaggca
     2641 atgggcccgc tctgttcgcg tgaaaaacgg atgctggggc tgatggtagg cgcgctggtg
     2701 ctgtggattt tcggcggtga ttatatcgat gccgcgatgg tcggttacag cgtggtggca
     2761 ctgatgctgc ttctgcgcat tatcagttgg gacgacattg tcagtaataa agcggcgtgg
     2821 aacgttttct tctggctggc ctcgcttatc accctcgcta ccggactcaa caacactggt
     2881 tttattagct ggtttggcaa actgttagca ggcagcttaa gcggttattc gccaacgatg
     2941 gtgatggtgg cgttgattgt ggtgttttat ctgctgcgct actttttcgc cagcgccacg
     3001 gcgtatacct ccgccctcgc accgatgatg attgccgctg cgctggcgat gccggaaatc
     3061 ccgctgccgg tgttctgcct aatggttggc gcggcaattg gtctggggag cattcttaca
     3121 ccatacgcca ccggacccag tccgatttac tacggtagtg gttatctgcc aacggcggat
     3181 tactggcgac tgggggcgat ttttgggctg atattcctcg tgttgctggt gattaccggc
     3241 ttactgtgga tgcccgtggt gttgctttaa gtgaggacgg tgccggaaat tgctctccgg
     3301 cacccaatag ttttacgcag ccggtaactc cgccagcggc cagcgcggac gcacgctaac
     3361 gccgagatcc gccgtcgcgc ctgctttaaa ccgcaccatt ccggcatagg cgatcatcgc
     3421 gccgttatca gtacaaaact ccggacgcgc gtagaacact tcgccgcggc gttttttcat
     3481 catttccgcc agcttcgccc gtaacgtgcg gttagcactc acgccgcccg ccatgaccag
     3541 tcgcttaaag cccgtctgat ccaacgctcg cttacactta atcatcaacg tatcgaccac
     3601 cgcatcttca aaggcgcggg cgatatcagc acgcgtctgg tcgtcggtgc cgttgtcacg
     3661 aatcgtgttc gccgcaaagg ttttcagacc agaaaagctg aaatccagcc ccagacggtc
     3721 ggtcatcgga cgcgggaaaa caaagcgccc ggcagtaccc tgagccgcca ttttcgacag
     3781 taacggtccg ccaggataat ccagccccag cagcttcgcg gttttatcaa acgcttcacc
     3841 ggcggcatca tcgatagact cgccgagcag ctcgtactga ccaatgccag tcacgctgat
     3901 taactgcgta tgaccgccgg acaccagcag cgcgacaaac ggaaattccg gcgggttatc
     3961 ttccagcatc ggcgctaaca gatgcccttc catatggtgt accgggattg ccggaacgtt
     4021 ccaggcaaac gccagagaac gccccacggt tgcgccaacc agcagtgcgc cgactaatcc
     4081 agggcctgcg gtataggcca cagcatcaat gtcttttgcc gttaagccag actcctttag
     4141 cgccgcctgg atcaacggta cggttttgcg cacgtgatcg cgggaggcca gttcaggcac
     4201 gacgccgccg tagtcagcgt gcaatttcac ctgactatac aattggttgg ctaacaaacc
     4261 tttttcatcg tcgtaaatgg cgatgccggt ttcatcgcag gaagtttcaa tacccagtac
     4321 acgcatgact tgttttacct cgctttatta ccgcgcagtg taggaccaat gcgggttgat
     4381 gtaaaacttt gttcgcccct ggagaaagcc tcgtgtatac tcctcaccct tataaaagtc
     4441 cctttcaaaa aggccgcggt gctttacaaa gcagcagcaa ttgcagtaaa attccgcacc
     4501 attttgaaat aagctggcgt tgatgccagc ggcaaaccga attaatcaaa ggtgagaggc
     4561 acatgccggt aattaaagta cgtgaaaacg agccgttcga cgtagctctg cgtcgcttca
     4621 agcgttcctg cgaaaaagca ggtgttctgg cggaagttcg tcgtcgtgag ttctatgaaa
     4681 aaccgactac cgaacgtaag cgcgctaaag cttctgcagt gaaacgtcac gcgaagaaac
     4741 tggctcgcga aaacgcacgc cgcactcgtc tgtactaatt ccccgagagc gttgctctcc
     4801 gatcagaccg agttgtagtt gtaaggccgt gcttccgaaa ggaatgcgcg gcttattttc
     4861 gtttatgaat tgctaaaaat cggggcctat ggctggacga atcccacgcg tattcattaa
     4921 tgatctactg gcacgcactg acatcgtcga tctgatcgat gcccgtgtga agctgaaaaa
     4981 gcagggcaag aatttccacg cgtgttgtcc attccacaac gagaaaaccc cgtcattcac
     5041 cgttaacggt gagaaacagt tttaccactg ctttggatgt ggcgcgcacg gcaacgcgat
     5101 cgacttcctg atgaactacg acaagcttga gttcgtcgaa acggtcgaag agctggcagc
     5161 aatgcacaat cttgaagtgc catttgaagc aggcagcggc cccagccaga tcgagcgcca
     5221 tcagaggcaa acgctttatc agttgatgga cggtctgaat acgttttacc aacaatcttt
     5281 acaacaacct gttgccacgt ctgcgcgcca gtatctggaa aaacgcggat taagccacga
     5341 ggttatcgct cgctttgcga ttggttttgc gccccccggc tgggacaacg tcctgaagcg
     5401 gtttggcggc aatccagaaa atcgccagtc attgattgat gcggggatgt tggtcactaa
     5461 cgatcaggga cgcagttacg atcgtttccg cgagcgggtg atgttcccca ttcgcgataa
     5521 acgcggtcgg gtgattggtt ttggcgggcg cgtgctgggc aacgataccc ccaaatacct
     5581 gaactcgccg gaaacagaca ttttccataa aggccgccag ctttacggtc tttatgaagc
     5641 gcagcaggat aacgctgaac ccaatcgtct gcttgtggtc gaaggctata tggacgtggt
     5701 ggcgctggcg caatacggca ttaattacgc cgttgcgtcg ttaggtacgt caaccaccgc
     5761 cgatcacata caactgttgt tccgcgcgac caacaatgtc atttgctgtt atgacggcga
     5821 ccgtgcaggc cgtgatgccg catggcgagc gctggaaacg gcgctgcctt acatgacaga
     5881 cggccgtcag ctacgcttta tgtttttgcc ggatggcgaa gaccctgaca cgctggtacg
     5941 aaaagaaggt aaagaagcgt ttgaagcgcg gatggagcag gcgatgccac tctccgcatt
     6001 tctgtttaac agcctgatgc cgcaagttga tctgagtacc cctgacgggc gcgcacgttt
     6061 gagtacgctg gcactgccat tgatatcgca agtgccgggc gaaacgctgc gaatatatct
     6121 tcgtcaggaa ttaggcaaca aattaggcat acttgatgac agccagcttg aacgattaat
     6181 gccaaaagcg gcagagagcg gcgtttctcg ccctgttccg cagctaaaac gcacgaccat
     6241 gcgtatactt atagggttgc tggtgcaaaa tccagaatta gcgacgttgg tcccgccgct
     6301 tgagaatctg gatgaaaata agctccctgg acttggctta ttcagagaac tggtcaacac
     6361 ttgtctctcc cagccaggtc tgaccaccgg gcaactttta gagcactatc gtggtacaaa
     6421 taatgctgcc acccttgaaa aactgtcgat gtgggacgat atagcagata agaatattgc
     6481 tgagcaaacc ttcaccgact cactcaacca tatgtttgat tcgctgcttg aactgcgcca
     6541 ggaagagtta atcgctcgtg agcgcacgca tggtttaagc aacgaagaac gcctggagct
     6601 ctggacatta aaccaggagc tggcgaaaaa gtgatttaac ggcttaagtg ccgaagagcg
     6661 atcgggaagc ccccgacagc cgcactgaga ggcagcggca aatatataag tacgccctcg
     6721 taattatcgt tggcggtaaa caaccgttgg atttcagcgt taacggctga aggacatcgg
     6781 gtcaatcgcc caacaccaac ctcatgaaat aagtgtggat accgtcttat ggagcaaaac
     6841 ccgcagtcac agctgaaact tcttgtcacc cgtggtaagg agcaaggcta tctgacctat
     6901 gccgaggtca atgaccatct gccggaagat atcgtcgatt cagatcagat cgaagacatc
     6961 atccaaatga tcaacgacat gggcattcag gtgatggaag aagcaccgga tgccgatgat
     7021 ctgatgctgg ctgaaaacac cgcggacgaa gatgctgccg aagccgccgc gcaggtgctt
     7081 tccagcgtgg aatctgaaat cgggcgcacg actgacccgg tacgcatgta catgcgtgaa
     7141 atgggcaccg ttgaactgtt gacccgcgaa ggcgaaattg acatcgctaa gcgtattgaa
     7201 gacgggatca accaggttca atgctccgtt gctgaatatc cggaagcgat cacctatctg
     7261 ctggaacagt acgatcgtgt tgaagcagaa gaagcgcgtc tgtccgatct gatcaccggc
     7321 tttgttgacc cgaacgcaga agaagatctg gcacctaccg ccactcacgt cggttctgag
     7381 ctttcccagg aagatctgga cgatgacgaa gatgaagacg aagaagatgg cgatgacgac
     7441 agcgccgatg atgacaacag catcgacccg gaactggctc gcgaaaaatt tgcggaacta
     7501 cgcgctcagt acgttgtaac gcgtgacacc atcaaagcga aaggtcgcag tcacgctgcc
     7561 gctcaggaag agatcctgaa actgtctgaa gtattcaaac agttccgcct ggtgccgaag
     7621 cagtttgact acctggtcaa cagcatgcgc gtcatgatgg accgcgttcg tacgcaagaa
     7681 cgtctgatca tgaagctctg cgttgagcag tgcaaaatgc cgaagaaaaa cttcattacc
     7741 ctgtttaccg gcaacgaaac cagcgatacc tggttcaacg cggcaattgc gatgaacaag
     7801 ccgtggtcgg aaaaactgca cgatgtctct gaagaagtgc atcgcgccct gcagaaactg
     7861 cagcagattg aagaagaaac cggcctgacc atcgagcagg ttaaagatat caaccgtcgt
     7921 atgtccatcg gtgaagcgaa agcccgccgt gcgaagaaag agatggttga agcgaactta
     7981 cgtctggtta tttctatcgc caagaaatac accaaccgtg gcttgcagtt ccttgacctg
     8041 attcaggaag gcaacatcgg tctgatgaaa gcggttgata aattcgaata ccgccgtggt
     8101 tacaagttct ccacctacgc aacctggtgg atccgtcagg cgatcacccg ttctatcgcg
     8161 gatcaggcgc gcaccatccg tattccggtg catatgattg agactatcaa caaactcaac
     8221 cgtatttctc gccagatgct gcaagagatg ggccgtgaac cgacgccgga agaactggct
     8281 gaacgtatgc tgatgccgga agacaagatc cgcaaagtgc tgaagatcgc caaagagcca
     8341 atctccatgg aaacgccgat cggtgatgat gaagattcgc atctggggga tttcatcgag
     8401 gataccaccc tcgagctgcc gctggattct gcgaccaccg aaagcctgcg tgcggcaacg
     8461 cacgacgtgc tggctggcct gaccgcgcgt gaagcgaaag ttctgcgtat gcgtttcggt
     8521 atcgatatga acaccgacca cacgctggaa gaagtgggta aacagttcga cgttacccgc
     8581 gaacgtatcc gtcagatcga agcgaaggcg ctgcgcaaac tgcgtcaccc gagccgttct
     8641 gaagtgttgc gtagcttcct ggacgattaa tcggtaggcc ggatcaggcg ttacgccgca
     8701 cccggcacta gtccctctgc acaaacgcca ccttt
//
LOCUS       DADLFA010000167         8721 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000167 DADLFA010000000
VERSION     DADLFA010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8721
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.169"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            132..197
                     /locus_tag="JQH65_003326"
     CDS             132..197
                     /locus_tag="JQH65_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6140637.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            336..947
                     /gene="ruvA"
                     /locus_tag="JQH65_003327"
     CDS             336..947
                     /gene="ruvA"
                     /locus_tag="JQH65_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAY6140638.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            956..1966
                     /gene="ruvB"
                     /locus_tag="JQH65_003328"
     CDS             956..1966
                     /gene="ruvB"
                     /locus_tag="JQH65_003328"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAY6140639.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(2016..2801)
                     /gene="znuB"
                     /locus_tag="JQH65_003329"
     CDS             complement(2016..2801)
                     /gene="znuB"
                     /locus_tag="JQH65_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HAY6140640.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLALDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLIAIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(2798..3553)
                     /gene="znuC"
                     /locus_tag="JQH65_003330"
     CDS             complement(2798..3553)
                     /gene="znuC"
                     /locus_tag="JQH65_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HAY6140641.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            3632..4564
                     /gene="znuA"
                     /locus_tag="JQH65_003331"
     CDS             3632..4564
                     /gene="znuA"
                     /locus_tag="JQH65_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HAY6140642.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLLGAKQV
                     TIAQLEDVKLLLMKSIHGDDDDHDHAEKSEEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4580..5902
                     /gene="mepM"
                     /locus_tag="JQH65_003332"
     CDS             4580..5902
                     /gene="mepM"
                     /locus_tag="JQH65_003332"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HAY6140643.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            6022..6993
                     /gene="lpxM"
                     /locus_tag="JQH65_003333"
                     /gene_synonym="msbB"
     CDS             6022..6993
                     /gene="lpxM"
                     /locus_tag="JQH65_003333"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HAY6140644.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMVGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLQIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRRPGEIQPYKRKDLYPIK"
     gene            complement(7082..>8721)
                     /locus_tag="JQH65_003334"
     CDS             complement(7082..>8721)
                     /locus_tag="JQH65_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAY6140645.1"
                     /translation="DGLPHVDFSRVDKFFTYADAFRESLPIISGELYFEAHQGCFTSE
                     SATKAHNRNMENKLHDAEFFTTITNNMTSILRSEFDEIWKEMLTLQFHDILPGSCISR
                     VYLETEKEYIKLEAKTEKIISDTQSTLLSKIDTSSYKDPHILFNTTCFARNEWININN
                     NWLKARVNSYGYAVIDPKNKIVNGLKAESRSIKNNYIKLLFSENGDLISLYDKRYGKE
                     YITENMHSEIRAYHEDAGFFAAWDFASNYRDGESYVLLAEKMTTVISGPKTTMTLIYH
                     YNSSYLRFAFTLTQDSPRVDVQTFIDWHEPNVSLKVKFPVSVQTSLAQCQIQFGVIDR
                     PTHSDDSFAFAKDEIPAHHWVDLSDRETGIALIAKNKYGYRVKDQTLELTLLRSQHKP
                     GEIVKTDNYDNFLINNFADIGKQKFIFSLFPHHGDYRVGGVINQAYIMNHPLQVVPVK
                     PHPGELPPSLSFFAIDTNSVIIETIKLAEDGDGIIVRLYESHGLEISAMLSWVCNYHY
                     VQYVDLQENKLDDSFYCNQYCNLEFKPFEIITLRLTQ"
BASE COUNT         2376 a         2011 c         2127 g         2207 t
ORIGIN      
        1 cacctataca tgtaaagacg atatctccaa tttcatattc agcagggtaa ttaatattca
       61 cgttgtcatt ccattgaaat agatacgaat atacgtaata gcataaaaaa attatttttt
      121 caggaggtgc aatgtatatt tttattacgc atttcttcac tgaatacgta atatttaaat
      181 atttgcttcc aatataacct gtagaataaa tcatactgcg ccatttttca gttcatcgag
      241 acacctcgca agttttcttc atccttcgct ggatatctat ccagcatttt tttatcatac
      301 agcattatct ttgattcatt acgcaggagc gtcatgtgat aggcagactc agaggcatca
      361 tcattgaaaa acaacccccg ctggtgttaa ttgaagtggg cggcgtaggc tatgaagtgc
      421 atatgccgat gacctgtttt tatgaactcc ctgaagcggg tcaggaagcg atcgttttca
      481 cccactttgt ggtgcgtgaa gacgcgcaac tgctgtacgg ttttaacaat aaacaagagc
      541 gcacattgtt caaagagttg atcaaaacca acggcgtcgg cccgaagttg gcgctggcga
      601 tcctctccgg aatgtcagcg cagcagttcg ttaatgccgt tgaacgtgaa gaagtggggg
      661 cactggtgaa actgccgggt attggcaaaa aaaccgccga acgcttgatt gttgaaatga
      721 aagaccgatt taaaggtttg catggcgatc tgtttacccc tgccgccgac ctggtactca
      781 catcacctgc cagcccggcg accgacgatg ctgaacagga agcggttgcc gcgctggtgg
      841 cgctgggcta taaaccacaa gaagcaagcc gcatggtgag caaaatcgct cgccctgacg
      901 ccagcagtga aacattaatt cgcgaagccc tacgcgccgc gttatgaggt aaaggatgat
      961 tgaagcagac cgtctgattt ctgccggtac cactttgccg gaagatgtag cagatcgcgc
     1021 cattcgccct aaattactgg aagagtatgt tggtcagccg caggttcgtt cacagatgga
     1081 gattttcatc aaagcagcga aactgcgcgg cgatgccctc gatcatttgt tgatttttgg
     1141 tcctccgggg ttgggtaaaa ctacgcttgc caacattgtc gccaatgaaa tgggcgttaa
     1201 tttacgcacg acttctggtc cggtgctgga aaaggcgggc gatctggcag cgatgctcac
     1261 taaccttgaa ccgcatgacg tgctgtttat tgatgagatc caccgtttat cgccagttgt
     1321 tgaagaagtg ctgtacccgg caatggaaga ctaccaactg gatatcatga ttggtgaagg
     1381 tccggcggca cgctccatta aaattgattt gccgccgttt accctgattg gcgcaaccac
     1441 gcgcgcaggt tcgctgacat caccgttgcg cgaccgtttt ggtattgtgc aacgtctgga
     1501 gttttatcag gtgccggatc tgcaatatat cgttagtcgc agtgcacgct ttatggggct
     1561 tgagatgagt gatgacggcg cgctggaagt tgctcgtcgc gctcgcggta cgccacgtat
     1621 tgccaaccgt ctgctgcgtc gagtgcgtga tttcgccgaa gtgaagcacg atggcaccat
     1681 ctcggcagat atcgctgctc aggcgctgga catgttgaat gtcgatgctg aaggtttcga
     1741 ttatatggac cgcaaattgt tgctggcggt aatcgataag ttctttggtg ggccggtagg
     1801 tctggataac ctggcggcgg ctattggcga agaacgtgaa accattgagg atgtgctgga
     1861 accttatttg attcagcaag gctttttgca gcgtacaccg cgtgggcgta tggcgacgac
     1921 gcgggcgtgg aatcactttg gcataacgcc gccagaaatg ccgtaagtcg gattggtcgc
     1981 tcgcgacgca tccgacaaat gtgttcagcg atagattagc tggcctgctt tttcatcata
     2041 ctgataataa ataacagtgc tgcacatagc accaccgacg gacccgccgg cgtatcgtaa
     2101 aatgcggaaa aggttaaacc gcccgttacg gcaaccatcc ccaccaacac agcgacacct
     2161 gccatctgtt ccggcgtgcg ggcaaagcga cgtgcagtag cagcaggaat aatcagcagt
     2221 gaagtaataa tcaacgcgcc aacaaatttc atcgctacac caatcgtcaa tgccgtcacc
     2281 agcatcaaca acaatttcac gcgctgtaat ttcacaccat caacaaacgc cagatccggg
     2341 ctaatcgtca tcgacagcaa attgcgccat tgccagaaca aaatagccac cacgatgacc
     2401 acgccaatcg caatagcgat gagatcttct ggcgtcactg ccagcaaatc accgaacagg
     2461 taagccatca aatcaacacg aatgttagac atcagactaa cgaccaccag gcccagcgac
     2521 agggcactgt gcgccataat ccctaataac gtgtcaagcg ccagctgtgg acgtttctcc
     2581 agccatacca gaccgcccgc caggagcagc gtaacggcaa ttaccgcata gaatggattc
     2641 acgtccagca acaaaccaaa cgcgacgcca agtaatgagg catgagccag cgtatcaccg
     2701 aaataagaca tacgacgcca gactacaaac gaacccagcg gacccgcggc acaggcgagc
     2761 atgatcccgg ctaaccaacc gggaaataat aattcaatca tgagcgatca tttccccgac
     2821 gcaaaacaat tcgtccctgt aaatcgtgac gatgattatg atgatggcga tagataccca
     2881 gttgttcagc accacgagga ccaaacatag aaataaactc cggatgtagg gaaacaactt
     2941 ccggtgtgcc ggaacaacaa atgtggtgat tcagacacag cacttcatcg gtttttgcca
     3001 ttaccagatg cagatcgtga gagaccatta aaacgccaca atccagttcg cgacgcaact
     3061 ggtcaataag gtcatataac gccacctggc catttacatc cacgccttga gtgggttcat
     3121 ccagcactaa taattgcggt cgatttaaca atgctcgcgc taacagtaca cgctgtgttt
     3181 cgccaccaga gagcttttgc atcggtgcgt taatcagatg cccggcctgg acacgtttca
     3241 gtgcaggcaa aatatcttct ttatgtgtgc cagggcgtaa gcgtaaaaaa cggtttacgg
     3301 tcagtggcaa cgtggtgtcg agatacagct tctgcggtac atagccgatg cgtagttttc
     3361 cgttgcgctt gataacccct tcatcgggtg ttaccagccc gagcactacc cgtaccagtg
     3421 tcgacttacc tgcgccattt ggcccaagta aagtcaaaat ttttccaggt ttaagttcca
     3481 gcgacacatc agagaggacg cggcgttggc caaaagaaac tgagacattt tccagggaaa
     3541 ccagacttgt catgttaatt ttagtcttgc agtagtcatg agatgttata atatcacact
     3601 tctcatattc attacgatga ttggtcgcat tatgttacat aaaaaaacgc ttcttttcgc
     3661 agcattatcc gccgctctct ggggaggtgc aacacaggcc gcagatgccg ccgttgtcgc
     3721 ttcgcttaaa cccgttgggt tcatcgcttc tgccattgct gatggtgtaa cagaaacgga
     3781 ggttttactg cctgacggcg cttcagaaca tgattattca ctgcgtccat cggatgtaaa
     3841 acgcttacag aacgcggact tagtcgtttg ggttggcccg gagatggaag cgtttatgca
     3901 aaaaccggta agcaaattac taggagcgaa gcaggtaacg attgcgcagc ttgaagatgt
     3961 gaaactgcta ctgatgaaaa gtattcacgg cgatgatgat gatcacgacc acgcggaaaa
     4021 aagtgaagaa gatcaccatc acggcgattt caacatgcat ctttggcttt ccccagagat
     4081 agcgcgggct acagcggttg caatccatgg aaaattagtg gaacttatgc cgcaaagtcg
     4141 agccaaactt gacgccaacc tgaaggattt tgaggcacaa ttagcctcaa ccgaaacgca
     4201 ggttggtaac gagctcgcgc cgctcaaggg gaaaggttat ttcgtttttc acgatgctta
     4261 cggctatttc gaaaaacagt tcggactgac accgcttggt cattttaccg ttaaccctga
     4321 gattcaacct ggcgcgcagc gtttacatga aataagaaca cagttggttg agcaaaaagc
     4381 aacctgcgtt tttgctgagc cacagttcag gccagcggtc gttgagagcg tcgcacgagg
     4441 gacatccgtt cgtatgggaa cgttggatcc cttggggacg aatatcaaac tgggtaaaac
     4501 aagctattca gaattcctga gtcaattagc caaccagtat gcgagctgcc tgaaaggaga
     4561 ttaatgagga agtgattacg tgcaacagat agcccgctct gtcgccctgg cgtttaataa
     4621 tttaccgcga ccacaccgcg ttatgttggg gtcgctcacc gttcttactc tggccgtcgc
     4681 tgtctggcgg ccctatgttt atcaccgcga tgccacgcca attgtcaaaa ccattgagct
     4741 ggaacagaac gaaattcgtt cgctcttacc tgaagccagt gagccgattg atcaagctgc
     4801 acaagaagat gaagccattc cccaggacga actggatgac aaaatcgccg gtgaagcggg
     4861 cgtgcatgaa tatgttgttt ccactggcga tacgctaagc agcattctca atcagtatgg
     4921 tattgatatg ggtgatatca cccaactggc tgcggccgac aaagaattgc gtaacctgaa
     4981 aatcggtcaa caactctcct ggacattaac cgcggacggc gaactgcagc gcctcacctg
     5041 ggaagtgtct cgtcgtgaaa cccgaaccta tgaccgtact gccgctaacg gttttaaaat
     5101 gaccagcgaa atgcagcaag gagagtgggt taacaatctg ctgaaaggta ccgtcggagg
     5161 gagctttgtt gccagcgcca gaaacgccgg tttaaccagc gccgaagtga gcgcagtgat
     5221 taaagccatg cagtggcaaa tggatttccg caaactgaaa aaaggcgatg aatttgcggt
     5281 gttaatgtct cgagagatgc ttgatggtaa acgtgagcaa agccagctgc tgggcgtacg
     5341 tttgcgttca gaaggtaaag attattacgc aatccgcgct gaggatggca aattctacga
     5401 ccgtaacggt actggtctgg cgaaaggatt cttgcgattc ccgacggcga aacagttccg
     5461 tatctcgtct aactttaacc cgcgtcgtac taatccggtg accggtcgcg ttgcaccaca
     5521 cagaggtgtt gatttcgcca tgccgcaggg tacgccagtg ctttcagtgg gtgacggtga
     5581 agtggtggtt gccaaacgca gtggcgcagc aggttattat gtggctattc gtcatggtcg
     5641 cagctacacc acgcgttata tgcacttgcg caagattctg gtgaaaccgg gacagaaggt
     5701 gaaacgtggc gaccgtatcg cgctttccgg taataccgga cgttcaaccg ggccgcatct
     5761 gcactatgaa gtatggataa accagcaggc cgtaaacccg ctgacggcaa aactgccgcg
     5821 taccgaaggg ctgaccggct ccgatcgtcg cgaattcctg gcgcaggcca aagagattgt
     5881 gccgcagcta cggtttgatt aattaacatc cattcgcagc cggtacgcag tcagtaccgg
     5941 ctttttttat ttggtgcggg gcaagttgcg ccgctacact atcaccagat tgatttttgc
     6001 cttatccgaa actggaaaag catggaaacg aaaaaaaata atagcgaata cattcctgag
     6061 tttgataaat cctttcgcca cccgcgctac tggggagcat ggctgggcgt agcagcgatg
     6121 gtgggtatcg ctttaacgcc gccaaagttc cgtgatccca ttctggcacg gctgggacgt
     6181 tttgccggac gactgggaaa aagctcacgc cgtcgtgcgt taatcaatct gtcgctctgc
     6241 tttccagaac gtagtgaagc tgaacgcgaa gcgattgttg atgagatgtt tgccaccgcg
     6301 ccgcaagcga tggcaatgat ggctgagttg gcaatacgcg gaccggagaa aattcagccg
     6361 cgcgttgact ggcaagggct gcagatcatc gaagagatga ggcgtaataa cgagaaagtt
     6421 atctttctgg tgccgcacgg ttgggccgtc gatattcctg ccatgctgat ggcctcgcaa
     6481 gggcagaaaa tggcagcgat gttccataat cagggcaacc cggtttttga ttatgtctgg
     6541 aacacggtgc gtcgtcgctt tggcggtcgt ctgcatgcga gaaatgacgg tattaaacca
     6601 ttcatccagt cggtacgtca ggggtactgg ggatattatt tacccgatca ggatcatggc
     6661 ccagagcaca gcgaatttgt ggatttcttt gccacctata aagcgacgtt gcccgcgatt
     6721 ggtcgtttga tgaaagtgtg ccgtgcgcgc gttgtaccgc tgtttccgat ttatgatggc
     6781 aagacgcatc gtctgacgat tcaggtgcgc ccaccgatgg atgatctgtt agaggcggat
     6841 gatcatacga ttgcgcggcg gatgaatgaa gaagtcgaga tttttgttgg tccgcgacca
     6901 gaacaataca cctggatact aaaattgctg aaaactcgca gaccgggcga aatccaaccg
     6961 tataagcgta aagatcttta tcctatcaaa taaaaaaaag cctctcaaat tattgagagg
     7021 ctgtactcga taaaaaatct caatgagtgt tcccactatt ttgagttttt aggttagtac
     7081 gctattgagt taatcgtagc gtgataattt caaaaggttt gaactcaaga ttgcaatatt
     7141 gattgcaata aaaactgtca tctaacttat tttcttgaag atcgacatat tgaacgtagt
     7201 gataattaca tacccacgaa agcatggctg aaatttctaa tccatgactc tcatataatc
     7261 ttacgattat accgtcgccg tcttcagcta attttattgt ttcgatgata acgctgttag
     7321 tatcgatagc aaaaaatgat agtgaagggg gtaattcacc cgggtgcggt ttgacaggga
     7381 cgacctgtaa gggatgattc attatatagg cttgattaat aacaccaccc acacggtaat
     7441 ctccatgatg aggaaataag gagaatatga atttttgttt accaatatct gcaaaattat
     7501 taatcaggaa attatcataa ttgtctgtct taacaatttc cccaggttta tgctgcgaac
     7561 gcagcaaagt taattcaagc gtctggtctt tgacacgata gccatatttg ttttttgcta
     7621 ttaaagctat gccagtttct cgatcagaaa ggtcgaccca gtgatgtgct ggtatttcat
     7681 ctttggcaaa agcgaacgaa tcatcgctat gcgttggtct gtcaattaca ccgaactgaa
     7741 tttgacattg tgcaagagat gtttgtactg acaccgggaa tttgactttg agagaaacat
     7801 taggttcgtg ccaatcaata aaagtttgga catcaacgcg tggactgtcc tgtgtcaggg
     7861 taaatgcgaa gcgaagataa gaggaattgt agtgataaat cagcgtcatc gtagttttag
     7921 gcccgctgat tacagttgtc attttttcag cgagtaaaac atatgactcc ccatcacgat
     7981 aatttgaagc aaaatcccag gccgcgaaaa aacctgcatc ttcgtggtat gcacggatct
     8041 cactgtgcat attttcagta atgtattctt taccatatct tttatcatat agcgaaatta
     8101 aatcaccgtt ttctgaaaaa agaagtttaa tgtaattatt tttaatactt cgagattctg
     8161 ccttcagacc atttacgatc ttattctttg gatcaatcac agcataacca taagagttta
     8221 ctcgtgcttt gagccagttg ttgtttatat tgatccattc gttacgagca aaacaggtgg
     8281 tattaaataa aatgtgcgga tctttataag aagaagtatc aattttactt aataaagtag
     8341 actgcgtatc agaaataatt ttctcggttt ttgcttctag tttaatatat tccttttcag
     8401 tttcaagata aactcttgaa atacatgatc cgggaaggat gtcatggaat tgcaaggtca
     8461 gcatctcttt ccatatttca tcaaactcac tgcgaagaat tgatgtcata ttattagtaa
     8521 tagttgtaaa aaattcagca tcatgtaatt tattttccat atttcgatta tgagctttag
     8581 ttgctgactc tgatgtaaag cacccttgat gagcctcgaa ataaagttca cctgaaataa
     8641 tgggcaagct ttcgcgaaaa gcgtcggcgt aagtgaagaa cttatcaaca cggctgaaat
     8701 caacatgagg taagccatca a
//
LOCUS       DADLFA010000168         8485 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000168 DADLFA010000000
VERSION     DADLFA010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8485
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.170"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(311..889)
                     /gene="mocA"
                     /locus_tag="JQH65_003335"
     CDS             complement(311..889)
                     /gene="mocA"
                     /locus_tag="JQH65_003335"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HAY6140646.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYVNQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIKRPNITNMRQALLMG
                     AHYSVEVENAEIILDIDTPDDFITVQKRYTKI"
     gene            992..1762
                     /gene="yqeC"
                     /locus_tag="JQH65_003336"
     CDS             992..1762
                     /gene="yqeC"
                     /locus_tag="JQH65_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HAY6140647.1"
                     /translation="MKSIVDPSALFIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRAMPLKAPDEHEPCIPKSSCCVIAVMGGHILGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            1810..3435
                     /locus_tag="JQH65_003337"
     CDS             1810..3435
                     /locus_tag="JQH65_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HAY6140648.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(3476..4408)
                     /gene="arcC"
                     /locus_tag="JQH65_003338"
     CDS             complement(3476..4408)
                     /gene="arcC"
                     /locus_tag="JQH65_003338"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAY6140649.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNVLREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(4456..5841)
                     /locus_tag="JQH65_003339"
     CDS             complement(4456..5841)
                     /locus_tag="JQH65_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HAY6140650.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(5894..7105)
                     /locus_tag="JQH65_003340"
     CDS             complement(5894..7105)
                     /locus_tag="JQH65_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HAY6140651.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(7163..8359)
                     /gene="dpaL"
                     /locus_tag="JQH65_003341"
     CDS             complement(7163..8359)
                     /gene="dpaL"
                     /locus_tag="JQH65_003341"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HAY6140652.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
BASE COUNT         1999 a         2061 c         2220 g         2205 t
ORIGIN      
        1 tagcttatac agatatcatt gagtgaccgg tgttgagaaa tccggtggtt tagggggata
       61 gagcaacaat cgcaccactc ttgccaggtt tggcgggaaa tataaaaaaa tgtgaggcgg
      121 ctcgaaactt aagggctttt tgtgaatgaa ctcacaggtg ctaaatgaga attaaaatca
      181 ctcctgaaaa ttattattgc cagcccatat atcacttttt ctcttttctc aaaatcagtg
      241 attccttatc attatgataa agagtgctgg agggattaaa tatttgaacg caatcgttta
      301 actacgtttt ttaaatttta gtataccttt tttgcactgt aataaagtcg tccggagtat
      361 caatatctaa aattatttct gcattttcta cttcgacgga ataatgagct cccataagga
      421 gtgcctgacg catattggtg atattggggc gcttgattgc ctgcatcagg catggttttg
      481 acactaaaat cggatggccg gggatgccat tatggagtgg cagtattgcg ccatcatttc
      541 gtaacgacca gatttttctg aaaatatcga tggtgagggt tggcatgtca ccgtgggtga
      601 gaaaacaatg ttctgtttgc actgcgggta ctgcggcctt tactgatgtt agtaaaccct
      661 gcgcataatc tgggttgtgg ataatagtga tattgctctg gttcacataa cgttcgtgca
      721 gttcattacc acgatagccg gtgactaaaa taattcggct acaaaactgc aacgcatttt
      781 tgatacttgt atcaagaatt gtcccctgtt gccagggtaa catcattttc cattgcccca
      841 ttcttgatga taatccggca gcggtaatta tacagtcgat ggctgacata gtggttccat
      901 tatttatagg ggctaataac gcctgataat tgcgttactt cctgatggat taagttgggg
      961 ataatttatc ccagagaggt cataaagact cgtgaaaagt atagttgacc catcggcatt
     1021 attcattgat ttaggtgcgc agaaacgtcc cactgtgatt tctgttgttg gtgctggggg
     1081 gaaaaccagc ttgctttttt ggctggcaga actgctccag gctagcggca ggcgtgtgtt
     1141 aatcacgacg acaacacata tgtttatgcc aacgtctcac tggcccgtgg ttttctgtcg
     1201 tgatcccgcc atgcttcctc atgcgtctct tacatccccc atttcatttt gttttcacag
     1261 ctggaaagcg aaccagggaa aagtgcaggg atttacgcca gaagcgattg atgcactggt
     1321 gcaacgacca gagtgtgacg taattctcat tgaagcagat ggctcgcgtg caatgccgtt
     1381 aaaagcgcct gatgagcacg aaccttgcat acccaaaagc agttgctgcg tgattgctgt
     1441 gatgggagga catattttgg gcgcgaaagt gagcacagaa aatgtccatc gctggtcgca
     1501 gtttgctgat attactgggt taacacctga tgcaaccttg caactgagcg atctcgttgc
     1561 gctggttcgc caccctcagg gggcgtttaa aaacgtaccg caaggttgtc ggcgagtctg
     1621 gttcattaac cgtttttctc aatgtgagaa tgcgattgca caaagcgagc tccttcaacc
     1681 gctgcaacaa cacgacgtag aggcaatctg gctaggcgat atacaagaac atcctgcaat
     1741 cgcgcgcaga tttgtgaatt agcggcccgg gtattccggg ttactgaaag gatatccatt
     1801 gaggttggta tgaatatttt cacagaggct gcaaaactcg aagagcaaaa ttgtccgttt
     1861 gcgatggcgc aaattgttga tagccgaggc tcgactcccc gccattctgc acaaatgtta
     1921 gtgcgcgccg atggttctat cgtcggtaca attggtggcg gaatggttga gcggaaggtg
     1981 attgaagagt cacttcaggc attgcaggaa cgtaagccgc gattattcca tggacgtatg
     2041 gctcgtaacg gtgcggatgc tgtcgggtca gattgtggag gtgcaatgtc agtatttatc
     2101 agcgtccatg gtatgcgtcc acgtctggtg ttgattggcg cggggcatgt caaccgggcg
     2161 atagcccaga gtgccgcgct attaggattt gatattgccg ttgccgatat ttatcgcgaa
     2221 agcctcaatc ctgaactatt cccaccatca accacgcttc tccatgctga gtcgtttggt
     2281 gcggcagtgg aagcactgga tattcgccct gataattttg tcctgattgc cacgaataat
     2341 caggatcgtg aagccctcga caaactcatt gaacagccca ttgcatggtt ggggttgctg
     2401 gcaagtcgcc gcaaggttca gcttttcctg cgtcaattgc gtgagaaagg cgtggctgaa
     2461 gaacatattg cccgtttaca tgcgcccgtt ggttacaaca taggtgcgga aacgccgcag
     2521 gagatcgcca tcagcgtgct ggcagaaata ttacaggtga aaaataacgc gccgggtggg
     2581 ctgatgatga aaccttctca tccttccgga caccagctgg tggtgattcg cggtgcggga
     2641 gatatcgcca gtggtgtggc gctacgtctg tatcatgcgg gttttaaagt gatcatgctg
     2701 gaagtggaaa aaccgacagt gattcgttgt accgtggcgt ttgcccaggc cgtgttcgat
     2761 ggcgaaatga cggtcgaagg cgtcactgct cgcctggcaa ccagctctgc ggaagcgatg
     2821 aaacttaccg aacgcggatt catccctgtg atggtagatc ccgcctgttc attgcttgat
     2881 gaactgaaac cgctttgcgt ggtggacgct attctggcga aacagaattt gggaacgcgg
     2941 gcagatatgg caccagtaac aatcgcgctt gggccgggct ttactgcagg gaaggattgt
     3001 catgcggtaa ttgaaacaaa tcgcgggcac tggctcggtc aggtgattta ctctggttgt
     3061 gcgcaggaga ataccggtgt tcctggcaat attatggggc ataccacccg acgggtgatc
     3121 cgtgctcctg ctgcaggcat tatgcgatcc aacgtgaaat taggcgatct ggtgaaagag
     3181 ggcgatgtga ttgcctggat tggtgagcat gaaattaaag caccgttgac ggggatggtg
     3241 cgtggcttgt tgaacgacgg cctggcagtg gttggtggtt ttaaaattgg tgatatcgat
     3301 cctcgtggtg aaaccgctga tttcaccagc gtttctgata aagcccgggc gattggcggc
     3361 ggcgtacttg aggcgttaat gatgttgatg catcagggcg tgaaagcgac aaaagaagtg
     3421 ctggaagtgg cttaaatgaa aatggccgga tggtattacc cacccggcct taaaattact
     3481 gacaaatcac cgttccggtt ttgccttcaa tcccttcttt cgctttgctc aatacggtaa
     3541 tcagtgcttc acgacctgcg cgagagcgag caaacgaggc ggcagcttcc actttaggca
     3601 gcatagaacc tttcgcaaaa tgaccttctt cgataaagcg ttcagcatca cttaatgaca
     3661 ggcgatcgag ccactgttca ttctctttac cgaagttaat ggccactttt tctaccgcag
     3721 tcagaatgat cagcatatcg gcatcaatca tttctgctaa acgggcgctg gcccagtctt
     3781 tatcgataac cgcgctggca ccacgcagat ggttaccttc acgaataaca gggataccac
     3841 caccgccaac ggtgatcacc acctggcctg catctaccag agctttaaca gtttcttttt
     3901 caatgatatc aaccggtttt ggcgaggcaa caacacggcg atagccgcga ccagcatctt
     3961 ctttcagagt gtaaccctgt tttgtcagtt gctctgcttc ttgctcggta aagaacgaac
     4021 cgatcggctt ggtcgggttg aggaatgccg gatcgttagc gtcgacttcg acctgagtaa
     4081 ccagcgttgc tacaggttta ttaatgcccc gagaaagcag ttcttctctt aacacgtttt
     4141 gtagatcgta accaatataa ccctggctta aggcaacaca gacagacatc ggcagcattg
     4201 gcgagtgtgc ttcagttttt gcggcagctt caaaggcctg gttaatcatg cccacctgcg
     4261 gcccgttacc atgagtgacg ataacttcat gtccttgggc aattaaatca acaattgcct
     4321 gagaagtaat ttttaccgct ttcatttgtc cggccaggtc atcgcccagc gcattcccgc
     4381 ccagggcgag aacaattttc ttactcattt ttcctcactc atcattcttg attgtgtata
     4441 gatccctgac agtggttaga gcacgggagg gacaaacggt ttgcgtcgca ggaaacgacc
     4501 tcgcccggct ttgcctgtaa aggtgccgtc acagaaaatc gtttcaccac gggataaggt
     4561 tctgacaatc gcgccctgac aggtaaaacc ctcccatggc gagtagtcgg cgttgtcgtg
     4621 gagatggcga tgctgaattt gttggctctg acgtgggtcg ataatcacca cgtcgccatc
     4681 ggaaccgggc gctaataatc ctttttgcgg ccacaggcca aacagcctgg cgggcattgc
     4741 gctggttaat tcaacaaagc gttccggtgt tatacgtccc gtcatcacgc cactggaaaa
     4801 cagtaactgc atgcggtttt ccacaccggg taagccattt gggcagcgac tgaaatcgcc
     4861 tttagaaatt tgcaggcgtt gagccatcga gaaggtgcag tgatcggtcg ccaccacgtc
     4921 aatcgcacca tcgctgatgc cacaccacag tttgtcctgc tcgcgtacgt tacgcagcgg
     4981 tgggctaaga atgaacttca tgccatcttc tgtatcgtaa ctgcgttcgt ccaacaggag
     5041 atattgtggg caggtttcaa cccagactgg ctggtgattc gcacgggcaa gacgcagata
     5101 atccagacct aagccgttag acaggtgcac gatatagagc ggggcgttac cggcaatttg
     5161 tgccaggtta atcatgcggg cgatggcttc cgcttcgcat tccagagggc gactcaaggc
     5221 atgatagcgc ggcgcggtta accctgcggc gataaattcc gcccgcttgc tggcgatagc
     5281 cgcatcattt tccgggtgca cggtggtcag cgcgccggat tcatgcaagc ggcgtaatgc
     5341 ctgcaaaacc tcgtcatcgt tgagtttgta ttgataggtt aaatagagtt taaaactgct
     5401 cagtccttcc tcgaccatca tcgggatttc gtcgaggatt gcgtgattaa tgtgctggat
     5461 cacaccgtga aagctgtaat cgatgaccgc tttatgggcg gcataaccac gataaacctc
     5521 cagttgatgg cgtaaccgac agccgtttgg gccaaatccc atatggtcaa taatggttgt
     5581 tgtaccgcca cacgcagctg cgcgggtacc ggtaaaaaaa tcatcacaac tgcgcgcgat
     5641 gccgacatca atattgaaat gcgtatggac atccacgccg cctgggaaaa cgtaacagcc
     5701 agtggcatca atttcttcat acgggagctg cggcgaaata ttgttgccca actggcgaac
     5761 aatcccgctt tcaatcagca aatcctgttt ggcttgtcca tctgcgttaa cgacagtgcc
     5821 gtttttgatc aatacgcgca tagcaaactc caaaaaccct ccggcgaacc ggaggggaat
     5881 aagtgaagag taattactct gttgccagcc agcttaacgg gattgcagcg tacatcgcgg
     5941 cacaggtcac caggtgagat ttccaggttt tttcgttagg tgcatgcgct tcaggttctt
     6001 tacccgggcc aaagccgatc accggaatgc cgtgacggcc catgatagat acgccgttag
     6061 ttgagaaggt ccacttatca acaaccggcg ctttgccaaa cagcccttcg taggcattca
     6121 ccagtgcttt aacggtgaag tgatcttctt ccactttcca ggtcgggaag tagcattcgg
     6181 ttgggtaaac caggccagtc caggacggac ggtcgtagtt gtacatagaa acaaccgcgt
     6241 tagctttctg tactgcaggc agggcgcgga tttcgtccag cgcgccttcc caggtttcgc
     6301 cccaggtcag acggcggtca atagagacgg cgcagctatc tgctacagcg caacggcttg
     6361 gggatgtgaa gaagatttca gaaacggtga gggtgccttt gccgaggaat tcgtcataac
     6421 ccagacgttg ggagagttct tgtaattcgc caagaatcgg acccattttg aaaatggcgt
     6481 tgtcaccgcg ttctggcgca gaaccgtggc agctaacacc ctgaacatca atacgaattt
     6541 ccatacgacc gcgctgacca cggtatatct ggcagtcggt tggttcggta ctgaccacaa
     6601 attccgggcg aatgccggat tgttcaataa tgtactgcca gcacagaccg tcgcagtctt
     6661 cttcctgcac agtaccggta accagcaggg tatattcatc ttccagaccg aggtctttaa
     6721 tgattttacc ggcataaacc atagatgcca tgccgccttc ctggtcggaa gtaccgcgac
     6781 caccgattag ctcatcagtt tccatgcctt cgtacggatc gaagtcccag tttttgatgt
     6841 tgccaatgcc gacggtatcg atatgagcgt ccattgccac cagacgcggg ccgtggccga
     6901 tataaccgag aacgttgccc atcgggtcga tttcaacttt atcgaagccg actttttcca
     6961 tctcttcttt aatacgatgt actacgcgtt tttcgtcgca gctttcactg ggaatagcaa
     7021 ccatgtcgcg caggaagcga gtcatgtccg cctggtaatc ttttgctttt tcaagaatca
     7081 gtttgaatgg aatattctta gccattattt ttctccagat tttttactcc ccgttttgca
     7141 gggagtagtt ccaatagggt gattaaggtg ctacagcgtg tttgccttcc cagacaactt
     7201 cgcggtagtg cttcacgtcg gtgtcgcctt cagtactgat aaccagcact acggcatctt
     7261 tgttcagcgc cagtttttcc atcaggcttt gacgttgcgg gtgataatga accgctgcga
     7321 gaacgcccaa accgacagcg ccggattcac cggagatgat gcgcgggtcg ttgccgtacg
     7381 gattacccag cacgcgcata cctaatgcgg caacgctgtc ctggcaggag atgaattggg
     7441 tggcgcagtt acgtaggatt tcccagccca gcgggttagg ttcgccacag gccaggcccg
     7501 ccatgatggt ggccatatca ccgccaacgt tgacgatgtc gcctttgacg ccggagcgat
     7561 aaatacagtc agctttgtca ggttcaacaa taatgctgtg cagattttgc gggctgtaga
     7621 cgtcgaccag ataacccagc acaccaccgg ccattgctcc gacaccggct tgcagcagaa
     7681 cgtgcgtcgg ggttacgccc atttcacgca tttgctcgac ggcttcatct gccagggttg
     7741 cgtagccttg catgatccag gttgggattt tggtgtaacc ttcccatgcc gtgtcctgta
     7801 ccacttccca gccgtgctgc tgcgcgtgtt gcatggtcag gcgaacggta tcgtcatagt
     7861 tcatatccgt gacgatgcac tcggcaccga ggttcagaat ggcgtcaacg cgttcctgag
     7921 cagaaccttt cggcatgtaa atcaccgcat tctgtccgag ttgctgtgct gcccacgcca
     7981 caccgcgccc gtggttgccg tcggtggtgg tcgcgaaagt cattttttcg ccgatggcat
     8041 ttttcaggtg ctcaaatgac agcgtttcga tatcaagatg atatttttca cacaataatt
     8101 gagcgatggc gtacgcaccg ccaagcattt tgaaggcgtt cagaccgaat cgttttgatt
     8161 cgtctttgac gagaattttc ttcacaccaa aaaggtttgc gagatcgtcc agcgcacaaa
     8221 gcggtgttgg gcgataacca gctattttct ggtggaactg gcgcgccagt ttggcctggc
     8281 tttgcgaaaa gagcggtgat gtttcgccgt tgaaaaattt gttatcggcg atatcaatct
     8341 tcaatgagaa aacggacata tcctatcctt ttggcctctg gaaatagagg gccgaccagc
     8401 tgatcggccc gaggggttat ttcacgcgtt cttgcgcttc tttcagcagt tgctcgagca
     8461 gtgcacctgg tttggcgtat ttacg
//
LOCUS       DADLFA010000169         8455 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000169 DADLFA010000000
VERSION     DADLFA010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8455
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.171"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..201
                     /gene="csgC"
                     /locus_tag="JQH65_003342"
     CDS             <1..201
                     /gene="csgC"
                     /locus_tag="JQH65_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="involved in autoagglutination of curliated cells;
                     not involved in production of curli fibers; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HAY6140653.1"
                     /translation="SLREGGSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIV
                     VTVSDGQALHLSQQWPPSSEKS"
     gene            322..633
                     /locus_tag="JQH65_003343"
     CDS             322..633
                     /locus_tag="JQH65_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY6140654.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQVASVKVQYIDKQKKLAVMNIEY
                     N"
     gene            729..1262
                     /gene="ymdB"
                     /locus_tag="JQH65_003344"
     CDS             729..1262
                     /gene="ymdB"
                     /locus_tag="JQH65_003344"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HAY6140655.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            1204..2685
                     /locus_tag="JQH65_003345"
     CDS             1204..2685
                     /locus_tag="JQH65_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HAY6140656.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRQMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2693..3850)
                     /gene="mdoC"
                     /locus_tag="JQH65_003346"
     CDS             complement(2693..3850)
                     /gene="mdoC"
                     /locus_tag="JQH65_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HAY6140657.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMLVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            4244..5779
                     /gene="mdoG"
                     /locus_tag="JQH65_003347"
     CDS             4244..5779
                     /gene="mdoG"
                     /locus_tag="JQH65_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HAY6140658.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGGAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            5772..8315
                     /gene="mdoH"
                     /locus_tag="JQH65_003348"
     CDS             5772..8315
                     /gene="mdoH"
                     /locus_tag="JQH65_003348"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HAY6140659.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKHWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
BASE COUNT         2134 a         2005 c         2211 g         2105 t
ORIGIN      
        1 tccctgcgcg aaggcggttc agggcaaagt cagacgaagc aagaaaagac cctctcattg
       61 cctgctaatc aacctattgc tttgacgaag ttgagtttaa atatttcccc ggaagatcgg
      121 gtgaaaatag ttgttactgt ttctgatgga caggcacttc atttatcaca acaatggccg
      181 ccctcttcag aaaagtctta atttgttgaa atgtcgagca taagatgaat ctggagagaa
      241 tggtctgctg cgaatcagcc aacctgaaag tatggataac acaaccctca aggatgacta
      301 atcattgagg aaatagaata aatgttcaga ccttttttaa actctcttat gctcggcagt
      361 ttgttttttc cttttattgc catcgcagga agcaccgcgc aagggggcgt gatccatttt
      421 tatggccaaa ttgtggaacc ggcatgtgac gtcagcaccc agtcatcacc cgtagaaatg
      481 aactgcccac agaatggttc tgttccggga aaaacctatt ccagcaaagc gttaatgagc
      541 ggcaatgtca aaaacgcgca agtagcctca gtaaaagtgc agtatattga taaacagaaa
      601 aagctggctg taatgaacat cgaatataac taagtttctg aacaacgcac gttgctgagt
      661 agaaaaatgc gatttaacca aaaagcctgc tgtacactta agaaacaaga aggtgttaag
      721 gaggcgttat gaaaacgcgt attcatgttg tgcagggtga tattaccaaa ctggccgttg
      781 atgtgattgt gaatgcggct aatccgtcat taatgggagg cggcggcgtc gatggggcca
      841 ttcatcgcgc agcgggtccg gccctgctgg atgcctgttt aaaagtcagg caacagcagg
      901 gcgattgccc tacggggcat gccgttatta cgcttgcagg cgatcttccc gctaaagccg
      961 tagtacacac cgtcgggcca gtctggcgtg gtggtgaaca aaacgaagac cagcttttgc
     1021 aggatgccta tctcaatagt ctgcgactgg tggcggcaaa cagctatacg tcagtggcat
     1081 ttcctgcaat cagtactggg gtttatggtt accctcgtgc ggcagcggct gaaatcgcag
     1141 taaaaaccgt ttcagaattt attacccgtc acgctttacc cgaacaggta tactttgtct
     1201 gttatgatga agaaaacgcc cacctctacg aaagactcct tacccaacaa ggagatgaat
     1261 gatttgcccc ggctggcgag cgcggtgctg ccactgtgtt cgcaacatcc cggtcagtgt
     1321 ggtcttttcc ctctggagaa aagtctggat gcgtttgccg cccggtatcg tctggccgaa
     1381 atggcagagc atacgctgga tgttcagtat tacatctggc aggacgatat gtcgggtcgg
     1441 ttactgtttt ccgccctgtt agccgcagca aagcgtggcg ttcgcgtccg tttgttgctg
     1501 gacgacaaca atacgcccgg acttgacgat attttacgct tgcttgacag ccatccccgc
     1561 attgaagtcc ggctttttaa tcctttctcg tttcgcttgc tgcgtccgct tggttatatc
     1621 accgactttt cccgtctcaa tcgccgtatg cacaataaaa gtttcactgt cgatggcgtg
     1681 gttaccttgg tgggaggacg aaatattggt gatgcctatt ttggggcagg ggaagagcca
     1741 cttttttcgg atttagatgt tatggcaata ggacccgtgg tagaggacgt tgccgatgat
     1801 ttcgcccgct actggtattg caaatcggtt tcacccttac agcaggtgct ggatgtcccg
     1861 gaaggtgaaa tggcggatcg catcgagtta cccgcctcct ggcataacga tgccatgacg
     1921 catcgttact tacgccaaat ggaatccagt ccgtttataa atcatctggt tgatggaacg
     1981 ttgccgctta tctgggcgaa aacacgttta ttaagtgatg atccggcgaa aggggagggc
     2041 aaggcaaaac ggcattcact gttaccgcag cgcctgttcg atatcatggg ctcacccagt
     2101 gaacgcatcg atattatctc ttcctatttt gtaccgacac gcgcaggtgt ggcgcaactc
     2161 ttacggatgg tgagaaaagg ggtaaagatt gcgatcctaa ccaattctct tgccgctaac
     2221 gatgttgctg tcgtccatgc cggatacgcg cgctggcgca aaaaattgct ccgctatggc
     2281 gtggaattat atgaactcaa gccgacgcgt gaacaaagta gtacgttaca cgatcgcggc
     2341 ataaccggta attccggtgc cagcctgcat gctaaaacct ttagcatcga tggtaaaacg
     2401 gtgtttatcg gctctttcaa tttcgatcca cgttcgacat tgctcaatac tgaaatgggc
     2461 ttcgtgatag agagcgaaac gctggcacag ttaattgata aacgctttat acagagccag
     2521 tatgatgcgg cctggcagct ccgtctggac aggtggggac ggatcaactg ggttgatcgt
     2581 catgcaaaga aagagattat tctcaaaaaa gaacccgcca ccagtttctg gaagcgggtt
     2641 atggtcagac tggcgtcgat attgcccgtg gaatggttat tgtaaatgtg gcttaacggg
     2701 ctggtgcttt atcgttctca cgcttaacaa ccggtttacc agaaaacaaa aacttcagta
     2761 acgggatgcg taaatgaatt tcatacagaa ttatcgcaat ccctactacg aatatcaggc
     2821 cacagagaaa accaagccag ttggaggtga tgtgcggtgt aatgtatgcg ccgaaaaaca
     2881 gcgttaacgg gtggtgaacc agatagataa atagcgatgc gttaacaaag taagtcaccc
     2941 gcgctgactg gaagttaagc aaacggtggc caaaggagaa gaccacattc accatccaca
     3001 gaccgaggac catggtgatc accgactcgg tttcgtacat ccaggcatcg ccactgccat
     3061 agcgttggtt gagtaaataa gcgacaaatg ccaatgctgc tgcaagggta cagccacgag
     3121 acggcgtggt aaacaaggct ttaagatgag ggaaaatgaa agccagtgca ccgaggataa
     3181 agaacggcaa ataaaatagc gtttgcatga caataaaatt gaacatgcca ttactcagaa
     3241 tgggcggata cacaataaaa atcgttcttc ttattaccgc atagccgatg ccgaggcata
     3301 aaaaaatcac cgatagtttt accatcgaga attttttatt cgttttatca gaattttcta
     3361 aattatttct gatgcgttta aatatccata cgcacagcgt cgtcatgact actaacacca
     3421 gtaaaaacca caggtgtgat attaattccc aggccaacgt attatatttg tcatacaatg
     3481 acagcccagg ccaactttcc gctttccctt tgacgtattg cagcataata aattgcggca
     3541 atgtcagtaa ggggatggct gttaacatcg ggatacctac acgttcgaca cgtactttcc
     3601 accatttttt caagggatag cgtaaaaaaa gcatgtagga aaagtagccg gatataacga
     3661 aaaataccag catgcggaac gagtggatga agtcattaaa aagggtcagc cacaatgacg
     3721 gttcggcgct attcacatgc catgtatggc tcgaatagat taaagaaata tgaaaaggga
     3781 tccctaacaa catcagccag gcgcggatgg agtcgaggaa atattcacgt tgcgcgggta
     3841 ctggattcat atatggttaa ctaatctcgg atttttcgtc ttatccctgt cgggttatgc
     3901 ctttaggctt gttgccatag cgacaccgac ctgaccgcgc caggcgcagg cttcaaggtt
     3961 tttatgcata gcataatcgc taccactaac cagaatggaa gcgtctgtaa gacggttgat
     4021 aaataaaatt gctggcaaac cctacacgaa gtcgatgctt ctgtctttag gagaagcacg
     4081 gaaagtgaaa acggttgcaa tcaggtgctt aatccatgag ccagcgtgct gaacgatacc
     4141 gggattctgt tgtcggaatg gctggttatc cattaaaata gatcggatcg atataagcac
     4201 acaaaggggg aagtgcttac taattatgaa acataaacta caaatgatga aaatgcgttg
     4261 gttgagtgct gcagtaatgt taaccctgta tacatcttca agctgggctt tcagtattga
     4321 tgatgtcgca aagcaagctc aatccttagc cgggaaaggc tatgaggcgc ccaaaagcaa
     4381 cttgccctcc gttttccgcg atatgaaata cgcggactat cagcagatcc agtttaatca
     4441 tgacaaagcg tactggaaca atctgaagac cccattcaaa ctcgagttct accatcaggg
     4501 tatgtacttc gataccccgg tcaaaataaa tgaagtgact gccaccgcag tcaaacgaat
     4561 caaatacagc ccggattatt tcactttcgg cgatgttcag catgacaaag acacggtaaa
     4621 agaccttggt tttgccggtt ttaaagtgct ttacccgatc aacagcaaag ataaaaacga
     4681 tgaaatcgtc agcatgctcg gggccagcta tttccgcgtg attggtgcag gtcaggttta
     4741 tggcctttct gcccgcggcc tggcaattga taccgccttg ccatcgggtg aagaatttcc
     4801 acgcttcaaa gagttctgga tcgagcgtcc aaaaccgact gataaacgtt taaccattta
     4861 tgcattgctt gactcgccgc gcgcgacagg tgcttacaaa ttcgtggtta tgccagggcg
     4921 tgacacggtt gtggatgtgc agtcgaaaat ctatctgcgc gataaagtcg gcaaactggg
     4981 gggtgcaccg ttaaccagta tgttcctgtt tgggccgaac caaccgtcgc ctgcaaataa
     5041 ctatcgtccg gagttgcacg actctaacgg tctctctatc catgccggta atggcgaatg
     5101 gatctggcgt ccgttgaata acccgaaaca tttagcggtt agcagcttct cgatggagaa
     5161 cccgcaaggc ttcggtctgt tgcagcgcgg tcgtgatttc tcccgctttg aagatctcga
     5221 tgatcgttac gatcttcgtc caagcgcatg ggtgactccg aaaggggagt ggggtaaagg
     5281 cagcgttgag ctggtggaaa ttccaaccaa cgatgaaacc aacgataaca tcgtcgctta
     5341 ctggacgccg gatcagctgc cggagccggg taaagagatg aactttaaat acaccatcac
     5401 cttcagccgt gatgaagaca aactgcatgc gccagataac gcatgggtgc aacaaacgcg
     5461 tcgttcaacg ggggatgtga agcagtcgaa cctgattcgc cagcctgacg gtactatcgc
     5521 ctttgtggtc gattttaccg gcgcagagat gaaaaaactg ccagaggata ccccggtcac
     5581 agcgcaaacc agcattggtg ataatggtga gatagttgaa agcacggtgc gctataaccc
     5641 ggttaccaaa ggctggcgtc tggtgatgcg tgtgaaagtg aaagatgcca agaaaaccac
     5701 tgaaatgcgt gctgcgctgg tgaatgccga tcagacgttg agtgaaacct ggagctacca
     5761 gttacctgcc aatgaataag acaactgagt acattgacgc aatgcccatc gccgcaagcg
     5821 agaaagcggc attgccgaag actgatatcc gcgccgtaca tcaggcgctg gatgccgaac
     5881 accgcacctg ggcgcgggag gatgattccc cgcaaggctc ggtaaaggcg cgtctggaac
     5941 aagcctggcc agattcactt gctgatggac agttaattaa agacgacgaa gggcgcgatc
     6001 agctgaaggc gatgccagaa gcaaaacgct cctcgatgtt tcccgacccg tggcgtacca
     6061 acccggtagg ccgtttctgg gatcgcctgc gtggacgcga tgtcacgccg cgctatctgg
     6121 ctcgtttgac caaagaagag caggagagcg agcaaaagtg gcgtaccgtc ggtaccatcc
     6181 gccgttacat tctgttgatc ctgacgctcg cgcaaactgt cgtcgcgacc tggtatatga
     6241 agaccattct tccttatcag ggttgggcgc tgattaatcc tatggatatg gttggtcagg
     6301 atttgtgggt ttcctttatg cagcttctgc cttatatgct gcaaaccggt atcctgatcc
     6361 tctttgcggt actgttctgt tgggtgtccg ccggattctg gacggcgtta atgggcttcc
     6421 tgcaactgct tattggtcgc gataaataca gtatatctgc gtcaacagtt ggcgatgaac
     6481 cattaaaccc ggagcatcgc acggcgttga tcatgcctat ctgtaacgaa gacgtgaacc
     6541 gtgtttttgc tggcctgcgt gcaacgtggg aatcagtaaa agccaccggg aatgccaaac
     6601 actttgatgt ctacattctt agtgacagtt ataacccgga tatctgcgtc gcagagcaaa
     6661 aagcctggat ggagcttatc gctgaagtcg gtggcgaagg tcagattttc tatcgccgcc
     6721 gccgtcgccg cgtgaagcgt aaaagcggta atatcgatga cttctgccgt cgctggggca
     6781 gccagtacag ctacatggtg gtgctggatg ctgactcggt aatgaccggt gattgtttgt
     6841 gcgggctggt gcgcctgatg gaagccaacc cgaacgccgg gatcattcag tcgtcgccga
     6901 aagcgtccgg tatggatacg ctgtatgcgc gctgtcagca gttcgcgacc cgcgtgtatg
     6961 ggccactgtt tacagccggt ttgcacttct ggcaacttgg cgagtcgcac tactggggac
     7021 ataacgcgat tatccgcgtg aaaccgttta tcgagcactg cgcactggct ccgctgccgg
     7081 gcgaaggttc ctttgccggt tcaatcctgt cacatgactt tgtagaagcg gcgttgatgc
     7141 gccgtgcagg ttggggggtc tggattgctt acgatctccc gggttcttat gaagaattac
     7201 cgcctaactt gcttgatgag ctaaaacgtg accgccgctg gtgccacggt aacctgatga
     7261 acttccgtct gttcctggtg aagggtatgc acccggttca ccgtgcggtg ttcctgacgg
     7321 gcgtgatgtc ttatctctcc gctccgctgt ggtttatgtt cctcgcgctc tctactgcat
     7381 tgcaggtagt acatgcgttg accgaaccgc aatacttcct gcaaccacgg cagttgttcc
     7441 cggtgtggcc gcagtggcgt cctgagctgg cgattgcact ttttgcttcg actatggtgc
     7501 tgttgttcct gccgaagtta ttgagcattt tgcttatctg gtgcaaagga acgaaagaat
     7561 acggcggctt ctggcgcgtt acattatcgt tgctactgga agtgcttttt tccgtgctgc
     7621 tggctccggt acgcatgctg ttccatacgg tcttcgttgt cagcgcgttc cttggctggg
     7681 aagtggtgtg gaattcaccg cagcgtgatg atgactccac ttcctggggt gaagcgttca
     7741 aacgccacgg ctcacagctg ctgttagggt tagtgtgggc tgttgggatg gcgtggctgg
     7801 atctgcgttt cctgttctgg ctggcaccga ttgtcttctc gttgatcctg tcaccgtttg
     7861 tttcggtgat ttccagccgt gccaccgttg gtctgcgaac caaacactgg aaactgttcc
     7921 tgatcccgga agagtattca ccgccgcagg tgctggttga taccgatcgc ttccttgaga
     7981 tgaatcgtca acgctccctt gatgatggtt ttatgcacgc ggtgtttaac ccgtcattta
     8041 acgctctggc aaccgcaatg gcgaccgcgc gtcaccgcgc cagtaaggtg ctggaaatcg
     8101 cccgtgaccg ccacgttgaa caggcgctta acgagacgcc agagaagctg aatcgcgatc
     8161 gtcgcctggt gctgctaagc gatccggtga cgatggcccg tctgcatttc cgtgtctgga
     8221 attccccgga gagatattct tcatgggtga gttattacga agggataaag ctcaatccac
     8281 tggcattgcg taaaccggat gcggcttcgc aataaaaacg tagttgcctg atgcgctacg
     8341 cttatcaggc ctacatcgtt cctgcaattt attgattttg caagattttg taggttgggt
     8401 aaggcgttca cgccgcatcc ggcaaaaaca acgagccaat aaaaataccg gcgtt
//
LOCUS       DADLFA010000170         8312 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000170 DADLFA010000000
VERSION     DADLFA010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8312
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.172"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(95..1765)
                     /locus_tag="JQH65_003349"
     CDS             complement(95..1765)
                     /locus_tag="JQH65_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HAY6140660.1"
                     /translation="MDNAELTIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEIHASAGDSFLGGEDFTHMLVDEVLKRA
                     EVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQEETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASDYLAIEIP"
     gene            complement(1849..2784)
                     /gene="rihA"
                     /locus_tag="JQH65_003350"
     CDS             complement(1849..2784)
                     /gene="rihA"
                     /locus_tag="JQH65_003350"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HAY6140661.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHIEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTDNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(2902..3627)
                     /gene="gltL"
                     /locus_tag="JQH65_003351"
     CDS             complement(2902..3627)
                     /gene="gltL"
                     /locus_tag="JQH65_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HAY6140662.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            complement(3627..4301)
                     /gene="gltK"
                     /locus_tag="JQH65_003352"
     CDS             complement(3627..4301)
                     /gene="gltK"
                     /locus_tag="JQH65_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HAY6140663.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNIFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            complement(4301..5041)
                     /gene="gltJ"
                     /locus_tag="JQH65_003353"
     CDS             complement(4301..5041)
                     /gene="gltJ"
                     /locus_tag="JQH65_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HAY6140664.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAIIMLVMTLVERKVRLPGNMGGK"
     gene            complement(5211..6119)
                     /gene="gltI"
                     /locus_tag="JQH65_003354"
     CDS             complement(5211..6119)
                     /gene="gltI"
                     /locus_tag="JQH65_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HAY6140665.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            6574..>8312
                     /locus_tag="JQH65_003355"
     CDS             6574..>8312
                     /locus_tag="JQH65_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAY6140666.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRDSDQIVISVGASGAIMGIAGAAIATQLASGAGTYHKNQR
                     RVFSLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGAGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLR"
BASE COUNT         2074 a         2052 c         2077 g         2109 t
ORIGIN      
        1 atgctctcgg caaaatcgcg atgttgttta gttgacgatg tgttttttgc gccgatgccg
       61 gatgcagcgt ttacgccgca tccggcagcc gtgtttatgg gatctcaatg gctaaataat
      121 cgctcgcccg tcgccgaact tcatccacgc gggccatgtc gtttgactgt agcgccgcat
      181 caaaatcggt gatcacccgg ccaatctctt ctcgctcgtc acccagcgct cgcgcccaca
      241 actcttccag tttggcttta aaggtgcgat tgatgagcat atcgcgcgga taaattttca
      301 atgcggataa ccgcgtccga ctctcttcaa tctgctgcgc gctcaatgtt accgggctgt
      361 ggttaatcac tctggactta acgctgccgt cttccagaag cacgtcaact tcaagcaacc
      421 cgttgatatc ataactaaag cgaatatcaa tcgactgata agccccggtt ttcttcaacg
      481 gcacatcgaa ggattcaacc agaatgttat ttttaacttt gtggttttct ccctgataga
      541 cgttaaccgt aattgaatcc tgctccgggt gcatggtcga gtaagtttct acacgcgaca
      601 ccggtactgt ggtgtttcgt tcaataatcg gcgagaaaat gccggaaacg ccctggcggt
      661 taacttcaac gcccaacgag taagggcaaa tatcagtgag gattacctct tcaatatctt
      721 cactgcgtaa gcggcaggcg gcctggattg ctgcgcccag cgcgacaatg gtgctcggat
      781 cgtaactttg atacggtaat ttgccaaaca gacgcacggc gattcgctgc accagcggca
      841 tttgtgacgc gccgccaacc agcaccagac tgtcgatttg actcggcttc agacgcgcat
      901 cgcgcaacgc ctgttcaatc ggcactcgca agcgattgag cagcggcaac cacaaatctt
      961 ccagttcgtt ctcgtaaaat tcgcattccc gcgtttcttc ctgatactgc cagcgaatgt
     1021 gcaatggcga ttgattgcta cattttgccg cttccacaca ggcgtacaag gctgccagtt
     1081 cactctcgtt aagcgtggtc ctggcaactt ccgcgcgttt taaaacctca tcgaccagca
     1141 tatgggtaaa atcttcgcca ccgaggaagc tatcgccagc ggaggcgtga atttcaatca
     1201 ccggcgtggc gtactcaagc accgtaacgt caaacgtgcc gccaccgaga tcaaaaacca
     1261 gcgagcgggt attttgttgc gtatgcaggc catatgccat cgcagctgct gtgggttcat
     1321 taattaagcg taccgcattt aacccggcta actccgcagc taaacgggta tgcttgcgtt
     1381 gttcatcgct gaaataagcc ggaacggaga tcaccacatc tttaatcgga cgttgcagaa
     1441 attcttcggc atcttctttt aatgagcgta ataccaaaga ggacagttct ggcgcgttaa
     1501 atgtgtcgct gcctaaccgc cagttggtat tactgcccat tgcgcgttta aataacgctg
     1561 ccgttttatc cggatgcgaa gtacgccgtg atacagccgg ttttccgact aaaatatgat
     1621 tattttcatc catgctaatt atggatggtg ttaaatattc accgaactta tttggaatta
     1681 attgcgcggc accgtctttc cagacggcaa ttaaactatt ggtagtgccg agatcgatac
     1741 caatggtgag ttctgcatta tccattgcat ttatatcttg aaagaaaaag agataaacag
     1801 attaagacca cccaggtaac gcgtggtgat cttaatcaat gacgtgtgtt aagcgtaaaa
     1861 tttcagacga tcagccagta aatcaacaaa gccctgacga tcaacatcga ccattacagt
     1921 ggcattcggt ttattgtcag tcagataata ataatcaaca accgtcatac cctgggtata
     1981 tttcccctgt gtttccacgc caacccagcg ctcaacagtg gtaaacaact cgggtttcaa
     2041 cagccaggcg atggtgcatg ggtcatgcag cggtgcaccg acaaagcccc atttttcgtc
     2101 tttatgatat tcgaggaaga aatccagcag ttcggcaaca atggttgaaa cagggttgcc
     2161 aatcgcgcgg aaacgctcgg tgtcttcaat gtggatttgc gctttatgag taacatccag
     2221 accggccatc accactggga tccctgactg gaagacaatt tctgccgctt ccgggtcaac
     2281 gtaaatatta aattcagccg caggcgtcca gttaccaagc cccattgcgc cgcccataat
     2341 cacgatacgg gcaattttgc tatgcagttc cggatggcta ttgagcagca aggcaacgtt
     2401 agtttgcggt ccggtagaca caatcgtgac aggttccgca ctttcgcgca gcgttttcgc
     2461 catcagctct accgccgtac agttttgcgg tgcgaacgtc ggttccggta atgccgggcc
     2521 atccagaccg ctttcgccgt gcacattgtc cgcgataatc aactcacgca ttaacggttt
     2581 taccgcgccg cctgctaccg gaatatcggt gcgattaagc aaggtcagca tacgcagaac
     2641 attgcgtaag gttttttctg gtgtctggtt tccggcggaa gacgtaattg ctttgacatc
     2701 aagctctggt gaggcgaggg cgagaactat tgcgatagcg tcgtcatgac ctgggtcgca
     2761 atctaacaga attggcagtg ccattgttgc tccttgttgt gtgcttcttt gcgacaaggg
     2821 taacgccagg atgtaacaga tacgaggggc gaaacgataa agcgtgagat ggcgcgcact
     2881 tgggtatgcg cgccagagtg attaatgcag gattttcgcg aggaagtctt tcgcgcggtc
     2941 cgatttcgga tcatcgaaga aagcgtcttt cggcgagtct tcgacaattt taccctcgtc
     3001 cataaagatc acccgattcg ccactttgcg ggcaaagccc atttcgtggg tcaccaccat
     3061 catggtcatt ccttcgttcg ccagttccac catcacgtcc agcacttcgt tgatcatctc
     3121 cggatccagc gccgatgtcg gttcgtcgaa cagcatcgca ataggatcca tacacaacgc
     3181 gcgagcgatt gccacacgct gctgctgacc gccggaaagc tgcgccggaa atttattggc
     3241 atgtgcggaa agtcccacgc gctccagcaa tttcaatgct ttttcacgag cgggcgcttt
     3301 atcgcgttta agcactttca cctgcgccag agtcaggttt tcaataatcg acaaatgggg
     3361 gaacaactca aaatgctgga acaccatccc gacgcgggaa cgcagctttg ccagatcggt
     3421 tttcttgtca ttaaccacga taccatcgac ggtgatttca ccttgctgca ctggttcgag
     3481 gccgttgacg gttttaatca gcgttgattt gccggaacca gacgggccgc aaaccaccac
     3541 cacttcgcct tttttcactt cggttgagca gtcggtcagc acctgaaagt gaccatacca
     3601 ttttgaaaca tttttcaggg taatcattat gctgtccttc ttttcaagta gctgaccaac
     3661 aacgacgcgc taagactaat aacgaaataa acaaatccgg caaacaggat catctcaacc
     3721 tgcgtaccat cacgctcacc aatggttgag gcggtacgga agaaatcggc cagagataac
     3781 acatacacca gtgaggtatc ctggaacagt acgatgccct gagtgagcag aagcggcacc
     3841 atcgcgcgga acgcctgcgg cagaataatc agtttcatcg actgccagtg agtcattccc
     3901 aacgccagcg cggcgctcga ctgaccacga gaaatacttt gaataccagc acggataatc
     3961 tctgaatagt aggccgcttc aaacatcgaa aacgccacca tcgccgaaat taaacggata
     4021 tcattttttg gtgataatcc cagcacgttt tgcagaaaac ctggcacgat caggtaaaac
     4081 cacagcaaaa ccataactaa aggaatcgag cggaatatgt taacgtaggc tttggcaaac
     4141 cacgctacgg gcgcaaagct ggacaagcgc atcaccgcca gcatggtgcc ccacaaaata
     4201 ccaatcacga ctgccgtgac ggtgattttc agcgtgatca ccagcccgtc gagcagatat
     4261 ggcagggaag ggacaatgga actccagtca aactcgtaca ttatttgccc cccatgttgc
     4321 caggcaggcg aactttacgt tcaaccagcg tcattaccag catgataatc gcgttaatca
     4381 acacatacgc cagcgtaata gcggtaaacg actcccaggc gtgggctgag taatcaagca
     4441 atttacccgc ctgcgccgcc atatccacca gaccaatagt cgaggcgatg gcggagtttt
     4501 tcaccaggtt catcatctcg gaagtcatcg gcgggacgat aacgcgataa gcattaggca
     4561 gcagtacgta tcgataagcc tgcggtagcg tcaggcccat cgccagcgcg gcattttttt
     4621 gcccacgcgg caacgactga atggcggcgc gtacctgttc gcaaacacgg gcggcggtaa
     4681 acagccccag gcagagcatg gatgaaagga aaaactgaat attgggatcc agctcggcct
     4741 taaaccacat gccgattttc tccggcaaca gctccgggat caccagatac caggtaaaga
     4801 attgcacaat caacggcacg ttgcggaaca gttcgacata caacgtaccc agaccggaga
     4861 gaaaacggtt tggaacggta cgtaaaatgc cgaaaaatga accgacgagg aaagcgataa
     4921 tccaggcgca gatcgacaaa gcgatcgtca cctgaaaacc actccagatc caaccaagat
     4981 aggtggtgtt gccgaacggg gcttgttgta aaaaaatacc ccagttccag tctatagaca
     5041 taaatctact ccagaaaaaa gagggtagca gcgttaactg ctaccctcga agattgttac
     5101 ccagcgtatt gcggttttca ggcccgatgg ggaacgacca tcaggcgtat agtctgtccg
     5161 tgctacgtaa caatcgagag ggctggaatt tccgcccctg gttcttgtaa ttagttcagt
     5221 gctttgtcat tcggttcttt gaacagtgct ttcatttcgt ctgacagttc gaaattcatg
     5281 ttcaggtttt tcggcggaat tggatttttg aaccatttat caaaccattt ttccgcttca
     5341 ccggaagtct gcacctgagc gatagtgtca tccatcagct ttttgaactg cggatcatct
     5401 ttacgcaaca tgcaaccgta ggcttcctga gactgcggct tgccgacgat atcccagttg
     5461 tctggtttct tcgctttcgc acgttcaccg gccagcaggg catcatccat cataaaggca
     5521 acggcacgac cgctttccag ggtgcggaaa gagtcaccgt gatctttggc gctgatgatg
     5581 cgcatattca ttttttgctc ttcattcagt ttgttgagca aaacttcaga ggtagtgccg
     5641 gaagtgacga ctacggcttt gtctttcagg tcggcaaaat ctttgatatc gccgcccttt
     5701 ttggtcagca ggcgcgtacc gaccacgaaa atagtatcag agaaagccgc ctgtttttgg
     5761 cgttcgacgt tgttggtggt agaaccacat tcaaaatcga aagtgccgtt ttgcagcagt
     5821 ggaatacggt tttgtgaggt aatcggaatc agttttacct gcaagtccgg tttgttgagt
     5881 ttctttttca ctgcttcaac aatggcgttg gagtaatcct gcgagtaacc caccactttt
     5941 tgctgattgt cgtaataaga gaaaggtact gaagattcac ggtgaccgac gacaatcaca
     6001 ccgtttttgg cgattttgtc cagcgtactg cctgctgccg gggcggcgtc atctgcctgt
     6061 gccagtcctg cggaaagcgc cagggcgagg attgctgtgg caggtttacg taattgcata
     6121 tccaactcct ttatcttctg cgttaagaac gcatggatac ccgttgtgag tgtttgtgtt
     6181 gttatcgtct gcaactttat tgtgcagtgt tgtgcctgtt aggtaacatt tagtttggct
     6241 aaatgtaaag atattgctgt tttattgttt gtttttgcga gatgcgccgc accattccga
     6301 agcaaaattc ttaaaccgca cctttttggt gctaccgctg atttgtggtg gtgcaacctg
     6361 gttgcgtcgc ggctgatttc atcgcttaca cagtaaataa agcaacttac atgccaaaaa
     6421 atacttaggg gcaaattaat tacgattgta attttaggct gaaaaagtca tatttcggac
     6481 agaattatct tttcggcatg ggacgtttgc cgggatgata actgcacacg ggaaaaatgc
     6541 tttttacctg tcttttcaag gaagaaacac cttatgtctg catcttcggt taagccgtta
     6601 aatgttcaat tacctgcaat aacccttatc ctttttgcac tctgtattgg gatattttgt
     6661 tatctcgcac aatggatgag ttatgaagag gtcgatcaat ccgcacttat ccatctcggc
     6721 gcaaacgttg cgcctcttac tttgtcgggt gaaccctggc gcttattgag cagtatcttt
     6781 ctgcacagta gtgtttctca tctgctgatg aatatgtttg cattcctggt cgtgggaggc
     6841 gtggcggaac agattctggg gaaatggcga ctcctgatta cctggttatt ctccggcgtc
     6901 tttggtgggc tcatcagcgc ctgttatgcg ttacgcgata gtgatcagat agtcatcagc
     6961 gttggggcat ccggggcaat tatgggaata gctggcgctg cgatagcaac acagcttgct
     7021 tcaggtgcgg gcacatacca taaaaaccag cggcgagtat tttctctgtt gggtatggtg
     7081 gcgctgacac tgttgtatgg tgcccggcaa acaggaatag ataatgcttg tcacattggc
     7141 ggcctgattg cgggtggcgc gttgggttgg ctgagcgcgc gtttatctgg gcaaaaccga
     7201 ctcgttacgg aaggcgggat tattgttgcg ggcagtcttc ttctgaccgg ggctatctgg
     7261 cttgcgcagc agcagatgga tgagtcagtt ttacaagtca ggcaaagcct gcgtgaagcg
     7321 ttttatccac aggagattga acaagagcga cggcaaaaaa agcagcagtt agcggaggaa
     7381 cgcaacgccc tcagggaaac attatccgct ccggtaagtc gtgaacaggc cagtggtgat
     7441 ttgctcgctg agattgccga tatccatgat atggcgatca gtcgggatgg taatatgttg
     7501 tatgccgcaa ttgaaaacac gaacagcatt gttgttttcg acctcggaca aaagaaaatc
     7561 ctgcatacct ttacagcgcc catagcgaaa gaaaagtcag tcaaacattg tggtggctgt
     7621 aaagatcagg gcgtcagatc gctggcatta agcccggatg aaaagttgat ttatgcgact
     7681 tcatttgaag cgaatgcgtt atcggtcatt aacgtggcga caggggagat tattcagtcg
     7741 attaccaccg gtgcacatcc tgacagcctt atcctctcgc gtgatggcac aaaagcctgg
     7801 gtgatgaatc gcaccagtaa tagtgtgtca gcgattgatc tggtgactta tcagcatgtg
     7861 gcggatatcc cgctggagaa atacgacggg gcggggacga gcggtaaacc aggcgcctgg
     7921 gttatggcac tttccccgga tgaaagaaca ttactggttc cgggagcagg cagaggtaac
     7981 atcgtgcgga tcaataccat cacgcatcag aaagaagact ttcccgcagg tgatgcgcgt
     8041 ggaacgatat cggcgatgcg ttttcgacct gaaaacggcg aggttatttt tgcagatagt
     8101 caggggattt cacgtataag cgtaggggat caacaagcca gcattatgac gcaatggtgt
     8161 agcaggagcg tttattccgt tgagggtatt agcccggacg gtcaatattt agcgttggtg
     8221 tcatatggcc tgcaaggtta tgtcatcctg ctcaatatta atgccgggca gattattggc
     8281 gtttatcctg ccagctacgt taatcacctt cg
//
LOCUS       DADLFA010000171         8213 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000171 DADLFA010000000
VERSION     DADLFA010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8213
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.173"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..467
                     /locus_tag="JQH65_003356"
     CDS             <1..467
                     /locus_tag="JQH65_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233146.1"
                     /note="type IV secretion system coupling protein; similar
                     to F plasmid TraD; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HAY6140667.1"
                     /translation="ERPKVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPDV
                     PEVVSGEDVTQAEQPQQPVSPVINDKKSDSGVNVPAGGIEQELKMKPEEEMEQQLPPG
                     ISESGEVVDMAAYEAWQQENHPDTWQQMQRREEVNINVHRERGEDVEPGDDF"
     gene            complement(476..874)
                     /gene="vapC"
                     /locus_tag="JQH65_003357"
     CDS             complement(476..874)
                     /gene="vapC"
                     /locus_tag="JQH65_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HAY6140668.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(874..1101)
                     /gene="vapB"
                     /locus_tag="JQH65_003358"
     CDS             complement(874..1101)
                     /gene="vapB"
                     /locus_tag="JQH65_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HAY6140669.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSADFMDNREQPGMQERESF"
     gene            1183..1602
                     /locus_tag="JQH65_003359"
     CDS             1183..1602
                     /locus_tag="JQH65_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005030230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HAY6140670.1"
                     /translation="MMSIAQVRSAGSAGNYYTNKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IEAHNQAVDFAVRHHPCGGTGLHTDDDGRTVRNGADR"
     gene            1589..6460
                     /gene="traI"
                     /locus_tag="JQH65_003360"
     CDS             1589..6460
                     /gene="traI"
                     /locus_tag="JQH65_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HAY6140671.1"
                     /translation="MLTGNLVMALFNHDTSRDQEPQLHTHAVVTNVTQHNGEWKTLSS
                     DKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVVGKHGMWEMPGVPVEAFS
                     GRSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQTLKETGFDIRAYRD
                     AAEQRAYTRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQFMYTDLLARTVGILPPEN
                     GVIERARAGIDEAISREQLIPLDREKGLFTSGIHMLDELSVRALSRDIMKQNRVTVHP
                     EKSVPRTAGYSYAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIA
                     ADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGA
                     ARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQRPATIISEPDRYVRYARL
                     AGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVTMTALSPVWLDS
                     RSRYLRDMYRPGMVMEQWNPETRSHDRYVTERVTAQSHSLTLRNAQGETQVVRISSLD
                     SSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPAT
                     LPVSDSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLY
                     SSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQKAGLHTPAQQAIHLALP
                     VLESKNLAFSMVDLLTEAKSFAAEGTGFADLGGEINAQIKRGDLLYVDVAKGYGTGLL
                     VSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAAARMILETSDRFTV
                     VQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFL
                     HDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQA
                     IAPGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINRDVERALSGLERVKPSQV
                     PRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDVPMTLYAAIVRDYTGRTP
                     EAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGQVQVMVPVLNTANIRDGELRRLST
                     WENNPDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVTLYTPDTI
                     RVGTGDRIRFTKSDRERGYVANSVWTVTAVSGDSVTLSEGQQTRVIRPGQERAEQHID
                     LAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQVYTDNRQGW
                     TDAINNAVQKGTAHDVFEPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDS
                     PARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGEGRVKGSGDA
                     QFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAITGGRVWGD
                     IPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLPDGKTE
                     QAVREIAGQERDRAAITEREAALPESVLREPQRVREAVREVARENLLQERLQQMERDM
                     VRDLQKEKTPGGD"
     gene            6480..7226
                     /gene="traX"
                     /locus_tag="JQH65_003361"
     CDS             6480..7226
                     /gene="traX"
                     /locus_tag="JQH65_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     acetylase TraX"
                     /protein_id="HAY6140672.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            7281..7841
                     /locus_tag="JQH65_003362"
                     /pseudo
     CDS             7281..7841
                     /locus_tag="JQH65_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
     gene            7972..8184
                     /locus_tag="JQH65_003363"
     CDS             7972..8184
                     /locus_tag="JQH65_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HAY6140673.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRG"
BASE COUNT         1873 a         2104 c         2658 g         1578 t
ORIGIN      
        1 aggaacgacc gaaggtcgct ccggagttta ttccgcgtga catcaacccg gaaatggaga
       61 accgtctgag tgccgtactt gccgcaaggg aagcagaagg tcgtcagatg gccagcctct
      121 tcgaaccgga tgtcccggag gttgtttccg gagaagacgt gactcaggct gaacagccgc
      181 aacagccggt gtctcctgtc atcaacgata agaagtcaga ttcaggtgtg aatgttccgg
      241 caggggggat cgagcaggag ctgaaaatga aaccggaaga agagatggaa cagcaactgc
      301 cacccgggat cagtgaatcc ggtgaagtgg tggatatggc cgcttatgag gcatggcagc
      361 aggaaaatca tccggacacc tggcagcaga tgcagcgtcg tgaagaggtg aacatcaatg
      421 tgcaccggga acgcggggag gatgttgagc cgggagatga tttctgatga acaggtcagc
      481 tccagtcttc agttctcagg ccgcccacac gttcaaattc ccgggtgtta ttagtcacaa
      541 taatcagccc ccgactgcgg gcatgacctg cgatcatttg atcaaatggc ccgacagggc
      601 gtccctgacg ggcaagttct gctcttatct ggccggtgtg tgtggctgca gcagcgtcgt
      661 aatccagaac atcaatgcgg gaaacaaatc cctcgatcac agcgagatta cgttcaggca
      721 tctggctttt ttctgcacca tatatcagct ccatcagggt gaccgaactg atacacattc
      781 tcccctggtt caggttaaaa cgctccctga cgctggcggg tttgttcttt atcgtaaaaa
      841 tgcagatgtt ggtatcgagc ataaacttca gcatcagaat gactcccttt cctgcatgcc
      901 gggctgttcc ctgttatcca taaaatcggc gctgacgctg tgtccgtcga accattcgtc
      961 ccatgtctct ccggctggcg taatgattct ggttcgtccg acagcaatca cttcaacgcg
     1021 ttttacgttt tccggcaatg caaccgcttt tggcagtctg accgcctggc tgcggttgct
     1081 gagaaatacg gtggtttcca ttatttgcct ccttatgtgg atatgtcttg tgtatatcca
     1141 ttgtgggatt gatgccggga tatgtcaaag ggatatacgt ttatgatgag tatcgcgcag
     1201 gtcagatcgg ccggaagtgc cggtaattat tataccaaca aggataatta ctatgtgctg
     1261 ggcagcatgg gagaacgctg ggccggccag ggggctgaac aactggggct gcagggcagt
     1321 gtcgataagg atgtctttac ccgccttctg gagggcaggc tgccggacgg ggcggattta
     1381 agccgcatgc aggatggcag taacaggcat cgtcccggct acgacctgac cttctccgct
     1441 cccaaaagtg tctccatgat ggccatgctc gggggggaca aacgtcttat tgaagcacat
     1501 aaccaggccg tggattttgc tgtccgtcat cacccgtgtg gaggcactgg cctccacacg
     1561 gatgatgacg gacggacagt cagaaacggt gctgaccggt aatctggtga tggcactgtt
     1621 taaccacgac accagtcgcg atcaggaacc acagttacac acgcatgcgg tggtgactaa
     1681 tgttacgcag cataacggcg aatggaagac actgagcagt gacaaagtgg ggaaaacggg
     1741 gttcattgag aatgtgtacg ctaatcagat tgcctttggc aggctctacc gggaaaaact
     1801 gaaagagcag gttgaggcgc tgggctatga aactgaagtg gtcggtaagc acggtatgtg
     1861 ggaaatgccg ggtgtaccgg tggaggcctt ttccggacgc tcacagacta tccgggaggc
     1921 cgtcggggaa gacgcctcgc tgaaatcccg ggatgtggcg gcgctggata cgcgtaaatc
     1981 caaacagcac gtcgatcctg aagtcagaat ggccgagtgg atgcagacgc tgaaggaaac
     2041 cgggttcgac atccgggcat atcgtgacgc agcggaacaa cgggcatata cccgcacgca
     2101 gacacccgga cccgcttcac aggacgggcc ggatgtgcag caggcggtga cacaggccat
     2161 tgccggatta agtgaacgca aagtgcagtt tatgtatacg gacctgctgg ccaggacggt
     2221 cggcatactg ccgccggaaa atggtgtgat tgaacgggca cgcgccggta tcgatgaggc
     2281 catcagccgt gagcagctta tccccctcga tcgtgagaag gggctgttca cgtccgggat
     2341 tcatatgctc gatgagctgt cagtccgggc actcagtcgt gacatcatga aacagaaccg
     2401 ggtgaccgta catccggaga aaagtgtccc ccggacggcc ggttacagct atgccgtcag
     2461 tgttctggca caggaccgtc cgtcgctggc cattgtgtcc gggcagggcg gtgcagccgg
     2521 gcagcgtgag cgggtggctg aactggtcat gatggcccgg gagcaggggc gggaggtgca
     2581 gattatcgct gctgaccgtc gctcgcagat gaacctgaag caggatgaac ggctgtccgg
     2641 tgaactgata accggacgtc gtcagttgct ggaaggcatg gccttcacgc cgggcagtac
     2701 cgttatcgtc gaccagggcg aaaaactctc cctgaaagag acgttaaccc tgctggatgg
     2761 tgccgcacgt cataacgtac aggtcctgat aactgacagc gggcagcgaa ccggtacagg
     2821 cagtgcactg atggccatga aggatgccgg ggtgaacaca tatcgctggc aggggggaga
     2881 acagcgaccg gccaccatca tcagtgaacc ggaccgttat gtccgctatg cccggctggc
     2941 cggagatttt gcggccagcg tgaaagccgg agaagagagc gtggcacagg tcagcggagt
     3001 acgggaacag gccatactga cacaggccat tcgcagtgag ctgaaaacac agggcgtgct
     3061 cggacacccg gaggtaacca tgaccgccct gtcaccggtc tggctggaca gccggagccg
     3121 ttatctgcgg gatatgtacc gcccgggaat ggtgatggag cagtggaacc cggagacacg
     3181 cagtcatgac cgctatgtga cagaacgtgt gacggcgcag agtcacagcc tgaccctgcg
     3241 gaatgcgcag ggcgaaacgc aggtggtgcg tatttcttcc ctggacagca gctggtcgct
     3301 gttccggccg gaaaaaatgc cggtggcaga cggtgagcgt ctgagggtga cagggaaaat
     3361 tcccggactc cgcgtctccg gcggtgaccg cctgcaggtg gcatccgtca gtgaagatgc
     3421 gatgacggtt gttgtgccgg gacgggctga accggccacc ctgcctgtga gcgattcacc
     3481 gttcacggca ctgaagctgg agaacggctg ggtggaaacg cccgggcatt ccgtcagcga
     3541 cagtgcgacg gtttttgcct ccgtcacaca gatggcaatg gacaatgcca ccctgaacgg
     3601 tctggcccgc agtggtcgtg atgtccggct gtattcctca ctggatgaaa cccgtactgc
     3661 ggaaaaactt gcccgccatc cttcctttac ggtggtttct gagcagataa aggcgcgtgc
     3721 cggtgagaca ttgctggaaa ccgctatcag tctgcagaaa gccgggcttc acacgccggc
     3781 acagcaggcc attcatctgg cccttcctgt gctggaaagt aaaaacctgg ccttcagcat
     3841 ggtggacctg ctgacagagg cgaagtcgtt tgctgcagaa ggaaccggtt ttgctgacct
     3901 gggaggggaa atcaatgcgc agataaaacg gggtgattta ctgtatgtgg atgtggcaaa
     3961 aggctatggc acaggcctgc tggtttcccg tgcgtcgtat gaggcagaaa agagcattct
     4021 tcgccatatt ctcgaaggta aggaggcggt cacgccgctg atggagagag tacctggcga
     4081 actcatggag aaactgacat caggacagcg cgccgccgcc cgtatgatac tggaaacgtc
     4141 cgaccgtttc acggtggtgc agggctatgc cggtgtgggt aagaccacac agttccgggc
     4201 ggtgatgtca gccgtgaaca tgctgccgga gagtgaacgt ccccgtgtcg tggggctggg
     4261 gcccacacac cgtgcggtcg gggagatgcg cagcgccggc gtggatgcac agacactggc
     4321 gtcctttctg catgacacgc agctgcagca gcgcagcgga gaaacgccgg atttcagcaa
     4381 cacgctgttc cttctcgatg agagctctat ggtgggcaat accgacatgg cacgggcata
     4441 cgccctgatt gcggccggtg gcggtcgggc tgtggccagc ggtgacacgg accagctgca
     4501 ggccatcgcg cccggtcagc ctttccgtct ccagcagacg cgcagtgctg ccgatgtggt
     4561 catcatgaag gagattgtgc gtcagacgcc ggaactgcgg gaggcggtat acagcctgat
     4621 taaccgggat gtggaaaggg cgctgtccgg gcttgagcgt gtgaaaccgt ctcaggtgcc
     4681 acgtctggag ggcgcatggg caccggagca ctccgtgacg gagttcagtc acagccagga
     4741 agcgaaactg gcagaagcgc agcagaaggc gatgctgaaa ggcgaggctt ttccggatgt
     4801 ccccatgaca ctgtatgcag ccattgtccg cgactatacc ggcaggacac cggaagcacg
     4861 ggagcagacg ctgattgtca cgcacctgaa tgaggaccgg cgcgtactga acagcatgat
     4921 tcatgatgca cgggaaaagg ccggagaact ggggcaggtg caggtcatgg tgcctgtcct
     4981 gaacacagcg aatatacgcg acggggagct gcgtcgtctc tccacctggg agaataaccc
     5041 ggatgccctt gccctggtgg ataatgtgta tcaccggatt gccggtatca gcaaggatga
     5101 cgggctgata accctgcagg atgcggaagg taacacgcgg ctgatttcgc cccgggaggc
     5161 ggtggctgaa ggcgtcacgc tgtacacccc ggacaccatc cgggtgggga ccggtgacag
     5221 gatacgcttc acgaagagtg accgggagcg cggttatgtg gccaacagcg tctggacggt
     5281 gacagcggtt tccggtgaca gtgtcacact ttctgagggt cagcagaccc gtgtgattcg
     5341 ccccggtcag gagcgggcag agcaacatat tgacctggcc tatgccatca ccgcccatgg
     5401 tgcacagggg gcgagtgaaa cctttgccat cgcgcttgaa ggcacggaag gtaaccggaa
     5461 actgatggcc ggctttgagt cagcctacgt ggccctgtcg cgtatgaagc agcatgtgca
     5521 ggtgtacacc gataaccgtc agggctggac ggatgccatc aacaatgccg tacagaaagg
     5581 aactgcccac gatgtgtttg agccgaaacc ggaccgggag gtcatgaatg cagagcggct
     5641 gttcagtacg gcgcgggagc tgcgggacgt ggcggcaggc cgtgccgttc tccgtcaggc
     5701 ggggctggcc gggggagaca gtcctgcacg gtttattgct ccgggacgta aatatccgca
     5761 accgtatgtg gcactgccgg cgtttgaccg taacggcaag tccgccggta tctggctgaa
     5821 cccgctgacc acggatgacg gaaacgggct gcggggattc agcggtgaag gtcgggtaaa
     5881 aggcagcggg gatgcgcagt tcgtggctct gcagggcagc cgtaacggag aaagcctgct
     5941 ggctgataat atgcaggatg gcgtccggat tgcccgtgat aatcctgaca gtggtgtggt
     6001 ggtaagaatc gccggtgaag gccgtccgtg gaatcccggt gccatcaccg gtggccgcgt
     6061 gtggggggat atcccggaca acagcgtcca gccgggagcc ggaaatggcg aaccggtcac
     6121 ggcagaggta ctggcacagc ggcaggctga agaggccatc cgccgtgaaa cggaacgtcg
     6181 cgcagatgaa attgtccgta aaatggcaga gaacaaacct gacctgccgg acggcaaaac
     6241 agagcaggct gtcagggaaa ttgccgggca ggagcgtgac cgggctgcca taactgaacg
     6301 ggaagccgcg ctgccggaga gtgtactgcg tgaaccacaa cgggtgcggg aagcggtccg
     6361 ggaagttgcc cgggaaaatt tgctgcagga gcgtctgcag cagatggagc gggatatggt
     6421 tcgtgacctg cagaaagaga aaaccccggg cggagactga tacaggaaga caaacactga
     6481 tgacaacgga taacacgaac acgacacgta acgattcact ggctgcacgg accgatacct
     6541 ggttgcagtc atttctggtc tggtcacccg gacagcggga tatcatcaaa acggtggcac
     6601 tggtgctgat ggtactggac cacatcaatc tgatattcca gctgaagcag gaatggatgt
     6661 ttctggccgg gcgaggagcc tttccgcttt ttgccctggt gtgggggctg aatctgtccc
     6721 gtcatgcgca tatccggcaa ccggccatta accggctgtg gggatgggga attattgccc
     6781 agttcgcata ttatctggca ggctttccct ggtatgaggg gaatatcctg tttgcctttg
     6841 cagtggcggc acaggtgctg acgtggtgtg aaacgcgcag tgggtggcgt acagcagcgg
     6901 ccatactcct gatggccctg tgggggcctt tgtccggcac cagttacggt attgccgggt
     6961 tgctgatgct ggcagtcagc aaccggctgt accgggcgga agacagagcg gaacgtctgg
     7021 cgctggtcgc ctgcctgctg gctgttattc cggcgcttaa ccttgccacc agtgatgcag
     7081 cggcggtggc cggtcttgtg atgacggtgc tgaccgttgg tctggtgttg tgtgccggaa
     7141 aatcattacc ccgtttctgg cccggagact ttttcccgac gttctatgcc tgtcatctgg
     7201 ctgtgctggg cgttctggcg ctgtgacggg tgtggtatct ttggccgcaa gaggatgatt
     7261 cgtcagaggc agaacacagc atgacagagc agaagcgacc ggtactgaca ctgaagcgga
     7321 agacagaggg aacagcgact gtccgcagcc ggaaaaccat catcaatgtc accacgccac
     7381 caaagtggaa ggtgaaaaag cagaagctgg ccgagaaagc ggcccgtgaa gcagagctgg
     7441 cggcaaaaaa agcgcaggcc agacaggcgc tgtccattta tctgaacctg cccacgctgg
     7501 atgaggctgt gaataccctg aagccctagt ggccgggatt atttgacggt gacacgcccc
     7561 ggcttctggc ctgcggtatc cgggacgtgt tactggaaga cgtggcgcag cggaatatcc
     7621 cgctctcgca taaaaaactg cgcagggcgc tgaaggccat cacccgttca gaaagctatc
     7681 tgtgtgccat gaaagccggt gcctgccggt atgacacgga agggtatgtg acggagcata
     7741 tttctcagga ggaggaagcg tatgcgggag cgcgtctggc gaaaattcgt cgccagaacc
     7801 ggataaaggc agaacttcag gccgtgcttg atgagaaata aaaaaagcct cccccagaga
     7861 gaggaggcag ggaaataagg cttaaaagga aataacttcc gcttccgaaa ggaagccagt
     7921 ccactgaatt atttatcaca ataatactga caactaaagg gggacaagaa aatgaatgga
     7981 ttcagaaaca gttcacggaa cggccaggtc tggcgttacc agcgtgccgg cgggcgggcc
     8041 gttattctgg aagtcagtgg acgctggatg gaagcggcag aagcatggcg acgggctgcc
     8101 tgtgttgcgc ctaggacaga ctggcagcag tttgcccgga aaagggctga acactgccac
     8161 cggcgctgcc ggggaagggg gtaacgaaaa aagcagcctt cattactcca gga
//
LOCUS       DADLFA010000172         8146 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000172 DADLFA010000000
VERSION     DADLFA010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8146
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.174"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            66..260
                     /locus_tag="JQH65_003364"
     CDS             66..260
                     /locus_tag="JQH65_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005009814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose carrier protein"
                     /protein_id="HAY6140674.1"
                     /translation="MQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDKYRK
                     VPPEPVHADIPVGAVSDVKA"
     gene            complement(399..1028)
                     /locus_tag="JQH65_003365"
     CDS             complement(399..1028)
                     /locus_tag="JQH65_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HAY6140675.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATARRYPLTPLLYTLIFLHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(1151..2797)
                     /locus_tag="JQH65_003366"
     CDS             complement(1151..2797)
                     /locus_tag="JQH65_003366"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HAY6140676.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGIAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDTCHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(2875..4215)
                     /locus_tag="JQH65_003367"
     CDS             complement(2875..4215)
                     /locus_tag="JQH65_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HAY6140677.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(4786..5505)
                     /gene="dcuR"
                     /locus_tag="JQH65_003368"
     CDS             complement(4786..5505)
                     /gene="dcuR"
                     /locus_tag="JQH65_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HAY6140678.1"
                     /translation="MSNVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(5502..7133)
                     /gene="dcuS"
                     /locus_tag="JQH65_003369"
     CDS             complement(5502..7133)
                     /gene="dcuS"
                     /locus_tag="JQH65_003369"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HAY6140679.1"
                     /translation="MRHSLPYRMLLKRPMKLSTTVILMVSAVQFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVVSGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYSLKTANNYKEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESLLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            7314..7544
                     /gene="yjdI"
                     /locus_tag="JQH65_003370"
     CDS             7314..7544
                     /gene="yjdI"
                     /locus_tag="JQH65_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HAY6140680.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVVTVVKVIDTCPSGALKYRHK"
     gene            7556..7828
                     /locus_tag="JQH65_003371"
     CDS             7556..7828
                     /locus_tag="JQH65_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY6140681.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            8055..>8146
                     /gene="ghoS"
                     /locus_tag="JQH65_003372"
     CDS             8055..>8146
                     /gene="ghoS"
                     /locus_tag="JQH65_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HAY6140682.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSL"
BASE COUNT         2021 a         1970 c         2084 g         2071 t
ORIGIN      
        1 cggttgtgtt agggatgatt ggctatgtac cgaatgttga acagtctacg caagccctat
       61 taggtatgca gtttattatg attgctctac caactctgtt tttcatggta acgctgattc
      121 tctacttccg tttctatcgc ctcaatggtg acacgctgcg caggatccag atccatctgc
      181 tggataaata tcgcaaagta ccgcccgagc ctgttcatgc tgatattccg gtcggtgcag
      241 tgagtgatgt gaaagcctga cgtggacaat agctaacgaa aatggcctga tgcgatatgt
      301 ttatcaggcc acgtcttaat gtaatgcttt ggatttgcaa attttgtaga ccggataagg
      361 aattcacgct gcatccggca tcaacaaagc gcacgttgtt atccggttat caagccaaag
      421 cgccgtagct ggcggcaatg aaagcgagcg aggaatatca ccgtcgttaa tgcgccaagc
      481 agcgcgcaga acatatcgga ttgcgtgtcc cactggtcgc cctgggtgcc gagaaaatca
      541 tccgctccct gacccattgc cagcgccgcc caccactcga ttaattcata cattgcgctt
      601 atcgccagcg ccacgcagca gactaggaac gccaccattt tacgtccgcg cacgtacatc
      661 ccgcgaacga gaatttctcg tgccaccagt gcaggcacca gcccctggaa aaagtgcccc
      721 agcttgtcat acggattacg gctcaacccc agccattcct gcacctcaaa accaacgggg
      781 actttcgcgt aggtgtattg tccgccgacc atcaggatga tggcgtgaag gaaaatgagc
      841 gtatagagca gcggcgttaa cggataacgt ctggcggtgg caagcagtag cggcacgaca
      901 ataatcaccg gtgtcacttc catcagccag gtgagtttgt catgggccga aatgccggta
      961 taaatcagga ttaatgttag cgccagtgcg ccggtgttaa gaattaacgg cttgagtgtg
     1021 cgggtcatgg tgagttcgca aatcagggaa aatactgact attcaccagc aagcgtgaaa
     1081 ttacaatcgc aaaagaggtg gcccaggggg atcacctggc agcatgctgc caggcgctgg
     1141 gccgaagagg ttacttagtg cagttcgcgc actgtttgtt gacgatttgc tggaagaagt
     1201 cgttaccttt gtcatcgacc aagataaacg ccgggaaatc ttctacttcg attttccaga
     1261 tagcttccat acccagctcc ggataagcga cgcattccag atgcttgatg ctctgctgcg
     1321 ccagtaccgc cgccggaccg ccgatgctac cgaggtagaa gccgccgtgt ttatgacacg
     1381 tgtcggtaac ctgctgactg cggttacctt tcgccagcat gatcatgctg ccgccgtggg
     1441 attgcagcag atccacgtag gagtccatac ggcctgcggt ggttgggcca agtgaacctg
     1501 atggataacc ggcaggggtt ttcgccggac ccgcgtagta gatcgggtga tctttgatgt
     1561 actgcggaag ttctttaccg gcgtcaatca gctctttcag cttggcgtgt gcaatatctc
     1621 ggcccacgat aatggtgccg gtgagcgaca aacgagtgga taccgggtat tgcgaaagct
     1681 gggcgaggat ctctttcatc gggcggttaa ggtcaacttt caccgcttcg ccttcaccgg
     1741 cctggcgcag ttcttgtgga atgtactggc ctgggttgtg ttccagtttt tcgatccaga
     1801 taccttcgcg gttgattttc gctttaatgt tacggtcagc ggagcaggag acgcccatgc
     1861 cgaccgggca ggatgcgccg tgacgtggca gacggataac gcgaatgtcg tgcgcgaagt
     1921 atttaccgcc aaactgcgcg ccaagaccga gtttctgggc ctcttccagc agttcctgtt
     1981 ccagctggac atcgcggaac gcctgaccat gttcgttccc ttccgtcggc agttcatcgt
     2041 aatagtgagc gcttgctaac ttgacggttt tcaggttggt ttccgcagac gtaccgccaa
     2101 tcacaaacgc gatatggtac ggcgggcagg ctgcagtacc gagggtacgc attttctcga
     2161 cgaggaagtt tttcagtttg ccgggagtca gcagggcttt ggtttcctgg tagagatacg
     2221 ttttgttggc agagccgccg cctttcgcaa cgcaaaggaa tttgtactca tcgccatcta
     2281 ccgcgtacag gtcgatttgc gcaggcaggt tagtgccggt gttgacctct ttgtacatgt
     2341 ccagcgccgc attctgtgaa tagcgcaggt tatcttcgat ataggtgtta tagacgcctt
     2401 tcgacagcgc ttcttcatca ccgccgccgg tccacacgcg ctggcctttt ttaccgacga
     2461 tgatcgcgat gccggtatcc tggcaggtcg gcagcacgcc tttggcggcg atttcggaat
     2521 ttcttaagaa ttgcagcgcc acgtacttgt cgttttcgct ggcttctgga tcgtgaagaa
     2581 tagccgcaac ctgtttctgg tgtgccgggc ggagcataaa agaagcgtcg tgaaaggctt
     2641 gctgcgccag cagggtcagg gcttctggtt ccactttcag gatggtttcg ccgtcgaagt
     2701 cggcaacgct aacgtaatcg gaagtgagta gatagtattc ggtattgtct ttccccatcg
     2761 ggaaaggtgc ctggtagata aagggtttgt ttgacatagc ttccagcctg taactctgta
     2821 tttgttattc gaaaatggcg tgccgcgtag tcacgggcgg cacgcaaagt gcatttataa
     2881 gaacccgtac atcgcggcga agatccagcc gaagacgcac gatacgctca caccaatcaa
     2941 ccccggcaga ataaagctgt ggttgatgac gaagcgaccg atgtgggtgg tgccggaacg
     3001 gtcaaactga atcgctgcca gatcgctcgg ataagtcggc aggatgtaat aaccgtagca
     3061 agccggtgct gaagccacga tgtatgccgg atcaacgccg atcgccaacg cgaccggaac
     3121 aatcgccgcc agcgccgcag cctgagagtt tacaaacttg gaaaccagca gcagaacaat
     3181 ggcataggcc cacggatact ctttcaccat ttcacccagt acgccctgaa tttcagacat
     3241 atgcgcaccg aacatggttt ctgccatcca tgcgataccg tacaccgcca cgatggcgat
     3301 cataccggaa cggaagactt cgttttttga gatagacgcg ggattggttt tggtcaggat
     3361 aataatcagc gccccggtca gcagcataaa catctgaata accagtacca tcgacagcgg
     3421 tttgccgccg aaggatggac gcaggtccga atcagcacca agaagggcga ctacagcgat
     3481 tgccccgagg aaaatccaca ttgccagcca gttgcttttc ggcagttttt tatccagcag
     3541 cgtcgcggta tcaccgtaaa catactcacg gttttccggt acggagatga atttctggaa
     3601 ctcttcgtct ttatccagat ctttaccacg gaaccagctg aagataccga tcgccaggat
     3661 accgattaac gtcgatggaa tggtgattgc cagcaggtcg aggaactcaa gatggcgacc
     3721 atcaaaggtg acattaccca gcatcgcaac cagagacacg accgcaaccg acaccggact
     3781 ggcgataatc cccatctgtg caccgataga acttgccgcc atcggacgtt ccggacggat
     3841 gttgttctta atggcgacgt cgtagatgat cggcagaatg gtgtaaacca catgacccgt
     3901 accgcaaaga atggtcagcg tacaggtcac aaacggcgcg acaattgaga catatttcgg
     3961 gttgcggcgc agcaacttct cggcaatttg cagcatgaca tcaagaccgc ccgaagcttg
     4021 caaggtcgcc gatgccgcca ccaccgcaat gataaccagc atgacatcaa ccggtggttt
     4081 acctggctga aggtggaaga cgaagaccag aatgaccaga ccgataccgc ctaataaacc
     4141 cagcgcgata ccaccctttc tggcaccata aaacagacat atcagtatta tgataagttg
     4201 gatagtaaat aacatgtgtg aaccctcgcg ataatcctat ttaaattttt gctgaataga
     4261 tcacagtcac gttctgtttt gtatgaactg ttttcagagc ctgactaaat atccgtttgg
     4321 tcgttaccgg ctttagcaaa tacctcacag taaatattgg ctgattaaag cagcggcaaa
     4381 tttatgcact gtgataagcg gctttttcag agagaaaaac tctctggcga tgggtttata
     4441 gtttgtactg cgcagatgtt aatgttttgt ttgctgttat cttattgata tttatcggtc
     4501 tattttttgt tctcagggca gatatcatta ggtttaaaga ttaatcttat ctacccattt
     4561 gtgggtgaaa atacacataa tgcgggtgac ataatagtta attaactttt gttagcgttt
     4621 tgaaattaaa aacaccgttc acctgaagag atattaattt ttagcgatga tggagggata
     4681 attatatttg atctggcaca agttttactg atgaaggatg taacttgtgc caggggtatt
     4741 ttgcattacg gtaataatta ttacttacag ataaccagcg atccgttatt ggcaatattg
     4801 tttcagtagt gagtagtgtt ctgcctgaat acggtaacgg taaaccggac gccccgtgac
     4861 gccataatgg atactggtga acaagatgtg gcagttgacc agccagatga ggtatttacg
     4921 gcaggaaaca cgcgagatgt taacctcgtt agccagctcg tcggttgaaa actcatagtc
     4981 ctgatgcgcg tcaatccact ggcacagcgt gcgtaacgtc tgcggcgtta agccttttgg
     5041 caagcgacga ggatcctgtt cgttggagct gctgccgtgg attagctgat caagctcggc
     5101 ctggtcataa tactgatgtt tttccagcgc cattttcttt tgccgccagc cggtgagcgc
     5161 ctcttcaaag cgggaagcct ggaagggttt gatcaggtaa tccacgacac cgtaatgcag
     5221 cgaatcttta atggttgccg catcggctgc ggaggagatg acaatcacat cacttttgca
     5281 acgcgcgtta tgcaggacag gcagtaaatc gagcccgttc tctttttgca tatagatatc
     5341 gagcaatatc aggtcgatag gcgtatcgct attgaagata atctctttgg ctttctccag
     5401 cgtcgaggct gttccacagc attgaaagcc tgggatttgt gctacgtatc ggcgattcag
     5461 ctccgcgacc attgcgtcgt catcgataat taatacattg ctcatctgtt tgacctctcc
     5521 ccgtcccagg gtatctggac aaaaaattgt gtgaaaatcc cgggttccga ttccacggcg
     5581 atgctgccgc cgagattttc tacctgttgt ttgacaagtg ctaaaccgac gcctcgctcg
     5641 cttccttttg tcgagacacc tttgtcaaaa atgtgatcga ttttgtcggg tgcgatcccc
     5701 ggtccatcat cgttaacttc gcagtgcagc cagccgtgac ggtagtgcaa tgttacgcta
     5761 atttcgccac cgggttccgg ccctaatgct tccagcgcgt tttctatcag atttcccaat
     5821 gtggtaatca gcgtcgcgac ctggtcctca ctgccgctgt ctggcagcag gctttcactg
     5881 tttaaaatca gcgtatggcc taaatcggtc gcgcggttaa tcttgctgat taaaaaacca
     5941 gcgataaccg gagatttgat cttacccagc agagagccaa tctcttcctt atagttattg
     6001 gctgttttga gactgtaatc ttccaactgc ttataactct tcagatgcaa taatccgaga
     6061 atcacatgca atttattcat aaattcgtgg gatcgttcac gaagtgcatc agcatagttg
     6121 accagaccgt cgagtcgctg catcagttta cgaacttcag ttttgtccct gaaggttgaa
     6181 atggcaccga tgataacgcc attactgcgc accggaacgg tgttgatcag taatagccgg
     6241 tctttaatcg taatctcttc gtcgcggcgc ggcgtaccgt cgcgtaacac ttccgagaca
     6301 tctaccacct gtgaccatga gtggcttagc gtcgacagtt tctcatcgtc ctgcgactta
     6361 cggtaattca gcaattcttg tgcggcatcg ttgatcagcg tgacctcgcc gcgatcgtcc
     6421 acggcaacga cgccttcttt gatagactgc aacatggcct ggcgctgctc aaacagcgtg
     6481 gagatttcgt agggttccag gccgaaaagg atttttttca gtaccttaac cagaatgcag
     6541 gtgccaatca gtccgaccag catgccaaat aataccgacc agataatgct ccagcgactg
     6601 tcattgattt gttgggtcac gcggcttaac tcaaggccgc tcaccaccac gccaatttgt
     6661 ttatgatttt catcgtagat gggggtaaat acgcgtaaag cctgcgccag aaaaccgcga
     6721 ttgatagcga cattttcttt gccattcagc gctttaagga tgtcatcacc tttaaatggc
     6781 tgaccaatac gctgggcttc aggatgcgag tagcgaagac tttgcatatc cgtaacgaca
     6841 ataaacagca gatcgttgcg tttgcgcacg gcttccgcga tggcctggat tccgctctcc
     6901 tgcggttttt tctgcaagcc ctgacggatt tccggcgagt cggcgagggt acgcgccact
     6961 gccagtgcct tgttggccag cccgtctcgt gtcatatcac tgatttgcga gaagtaaatc
     7021 agatgcacca ccaatagcac cgagaactgt accgcgctga ccattaagat cactgtggta
     7081 ctcaatttca tcggacgttt gagtaacatg cggtagggca atgaatgtct catcagcttc
     7141 cttgtgtgac aaatttctta agcattatct ctgatgaggc gggtaattca aagggcgtaa
     7201 gaatgattgg ctatataggg gaagagactc tggcaacgga aactgccagt gctgtatgaa
     7261 gattccaggg ctatgcttat agcgataatc atactgatga gagagggaaa gtcatggatc
     7321 aggcgctact ggacgagggt tatcgctgtt ataccggcga aaagatcgat gtctatttca
     7381 acactgcgat atgtcagcat tctggcaatt gcgtacgtgg caacggcaag ttatttaatc
     7441 tcaaacgaaa gccgtggatc atgccggatg aagtcgacgt cgtcactgtg gttaaagtga
     7501 ttgatacgtg cccgagcggc gcgctgaaat accgtcataa ataagcgagg gtaaaatgga
     7561 aatacgcgaa ggccacaata aattttacat taatgacgaa cagggcaagc aaatcgctga
     7621 aattgtcttt gtgccgaccg gagagaattt agcgattatc gaacataccg atgtcgatga
     7681 aagcctgaaa gggcagggga ttggtaaaca gctggttgcg aaagtcgtgg aaaaaatgcg
     7741 tcgggaaaaa cgaaaaatta tcccactatg cccatttgcg aaacacgaat ttgataaaac
     7801 gcgggagtat gatgatattc gcagttgatg ggagagtaca gagtcacgat atttttcatg
     7861 ctctccgcgg agtgatgcag gagagcatct gaagggtagg gggatgcaca aagaatgggc
     7921 agagagcgcg tttttttgtc ccacgtcatc ccctttactg agcaaaaaaa agaatatctc
     7981 ctatatgaga atcatcaatc ggggttaata agttttgcgt ccccagagca ttcaatattg
     8041 ataggagtca tattatggaa ggtaaaaaca agttcaatac ttatgttgtt tcttttgatt
     8101 atccatcatc ttattcctca gtgttcttaa ggttaagatc attgat
//
LOCUS       DADLFA010000173         8113 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000173 DADLFA010000000
VERSION     DADLFA010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8113
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.175"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(133..209)
                     /locus_tag="JQH65_003373"
     tRNA            complement(133..209)
                     /locus_tag="JQH65_003373"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(173..175),aa:Asp,seq:gtc)
     gene            complement(342..1073)
                     /gene="dnaQ"
                     /locus_tag="JQH65_003374"
     CDS             complement(342..1073)
                     /gene="dnaQ"
                     /locus_tag="JQH65_003374"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAY6140683.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            1138..1605
                     /gene="rnhA"
                     /locus_tag="JQH65_003375"
     CDS             1138..1605
                     /gene="rnhA"
                     /locus_tag="JQH65_003375"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HAY6140684.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1602..2324)
                     /locus_tag="JQH65_003376"
     CDS             complement(1602..2324)
                     /locus_tag="JQH65_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY6140685.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2358..3113
                     /gene="gloB"
                     /locus_tag="JQH65_003377"
     CDS             2358..3113
                     /gene="gloB"
                     /locus_tag="JQH65_003377"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HAY6140686.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            3185..4543
                     /gene="mltD"
                     /locus_tag="JQH65_003378"
     CDS             3185..4543
                     /gene="mltD"
                     /locus_tag="JQH65_003378"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HAY6140687.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRDSLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4592..5215)
                     /locus_tag="JQH65_003379"
     CDS             complement(4592..5215)
                     /locus_tag="JQH65_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAY6140688.1"
                     /translation="MSGLPQGRPTFGAAQNVSAVVAYDLSAHMLDVVAQAAEARQLKN
                     ITTRQGYAASLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSP
                     GHPVRDIWLQAVEALRDTSHVRNYASGAWLTLINEANLIVDNLITDKLPLEFSSWVVR
                     MRTPEALVDAIRIYQQSASTEVRTYFSLQTDGSFTSDIIMVEAHKAA"
     gene            complement(5219..6019)
                     /locus_tag="JQH65_003380"
     CDS             complement(5219..6019)
                     /locus_tag="JQH65_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAY6140689.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            6260..7174
                     /locus_tag="JQH65_003381"
     CDS             6260..7174
                     /locus_tag="JQH65_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY6140690.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDREIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(7171..7953)
                     /gene="dkgB"
                     /locus_tag="JQH65_003382"
     CDS             complement(7171..7953)
                     /gene="dkgB"
                     /locus_tag="JQH65_003382"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HAY6140691.1"
                     /translation="MAIPAFGLDVVISSVKTALELGYRAIDTAQIYDNEAAVGQAIAE
                     SGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPNDEVSVEG
                     VMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQNRKVVAW
                     AKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSVIPSSTKR
                     KNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT         2173 a         1948 c         1967 g         2025 t
ORIGIN      
        1 gtctaccata tgtgattaac acatttacag cataacaaaa ccatagaccg aagaaatagc
       61 gggatttaaa tgcaaaaaaa ccgcaaaatt aaaaatcttg cggttctctg aactcatttt
      121 catgagtgaa tttggcggaa cggacgggac tcgaacccgc gaccccctgc gtgacaggca
      181 ggtattctaa ccgactgaac taccgctccg cgttgtgttc cgttgggaac gaggcgaata
      241 ttacggattg cctcaccctt cgtcaacggt ttttctcatc ttttgaatcg tttgctgcaa
      301 aaatcgccca agccgctatt tttagcgcct tttacaggta tttatgcccg ccagaggcag
      361 cttccgccct tcttctccac cagatcaaga cgggcttcat gagctgcaag ctcttcatct
      421 gtcgcaaaaa caacgcgtaa cttacttgcc tgacgtacaa tgcgctgaat tgttgcttca
      481 ccttgttgct gctgtgtctc tccttccatc gcaaaagcca tcgacgtttg accaccggtc
      541 atcgccagat aaacttccgc aaggatctgt gcatcgagta atgccccgtg tagcgttcgt
      601 ttactgttat ctatttcgta gcgggcacat aacgcatcga ggctgttgcg cttaccagga
      661 aacattttcc tcgccaccgc aaggctatcg gtgaccttac agaaggtatt ggtcttcgga
      721 atatcgcgct taagcaacga aaactcgtag tccataaagc cgatatcgaa cgctgcgtta
      781 tggatcacca actccgcgcc gcggatatag tccatgaact catcggctac ttcggcaaac
      841 gtgggcttat cgagcaaaaa ttcatcggca ataccatgta cgccaaaggc ttccggatcc
      901 accagccgat cgggtttgag ataaacatgg aagttattgc ccgtcaggcg acggttcacc
      961 acttcaacgg caccaatctc aatgatcttg tggccttcat agtgcgcacc aatctggttc
     1021 ataccggtgg tttcggtatc gagaacgatc tggcgtgtaa ttgcagtgct catagcggtc
     1081 atttatgtca gacttatcgt tttacagttc gattcaatta caggaagtct accagagatg
     1141 cttaaacagg tagaaatttt caccgatggt tcgtgtctgg gcaatccagg acctgggggt
     1201 tacggcgcta ttttacgcta tcgcggacgc gagaaaacct ttagcgcagg ctacacccgc
     1261 accaccaaca accgtatgga gttgatggcc gctattgtcg cgctggaggc gttaaaagaa
     1321 cattgcgaag tcattttgag taccgacagc cagtatgtcc gccagggtat cacccagtgg
     1381 atccataact ggaaaaaacg tggctggaaa accgcagaca aaaaaccagt aaaaaatgtc
     1441 gatctctggc aacgtcttga tgctgcccta gggcaacatc aaatcaaatg ggaatgggtt
     1501 aaaggccatg ccggacaccc ggaaaacgaa cgctgtgatg aactggctcg tgctgcggca
     1561 atgaatccca cactggaaga tacgggctac caagtcgaag tttaagcttg tggtttacga
     1621 cattgccggg tggctccaac cgcttggcga attcgtggct tgtttttact ctgtttcatc
     1681 gggtttagcg ttaaaggaat agtccgtttc cgggcaacaa taagttgtaa gcaaccaagc
     1741 gcaggaatat gcgcattcaa tagttttcct ccgtgtttgt tccacgggag aacgtggaaa
     1801 cggctggcgt gcagcacttc aaaattcaac aaagagagcc agtccagttg ccgcatcaga
     1861 gtaaacatcc ggctgttata gggcgaggtt tttcgcaata ccggcacaag tttgcgtaat
     1921 cccattaaac taatgggatt gaagccacta atcaccagcc agccatcatc aatcaatacc
     1981 cgatcggttt cacgcaataa acgatgcgga tcggtgcacc acggcaatgt atgtgccagt
     2041 agacaaacat caacggattt atcggcaaaa ggaagatgaa gtggatccgc ctgcacctgg
     2101 acgggcattc cttgcgcaga aacattcact tgatgagaaa ccgcgcacgc ttcgcaattg
     2161 atttctgcgc ttaaattgcc aatcttaagc agatgaaaac catacatttt agtgaaccac
     2221 gggttgagct ggcgctccag cgccttgcga taaagctctc cccagggcaa atcgccccag
     2281 cgatcaggag ccacgacagt ttgagggact cttgccggtt tcatcacaac cttccgtttc
     2341 acactaagag gtaatctatg aatcttaaca gtattcccgc ctttgatgac aattacatct
     2401 gggttttgaa tgatgaagca ggtcgctgcc tgattgtcga tcccggagac gcagagccag
     2461 tattaaacgc cattgccgcc aataactggc aaccggaggc catatttctc acccaccatc
     2521 atcacgatca cgttggcggc gtaaaagaac tggtggaaaa gtttccgcaa attgtggtgt
     2581 atggtccaca agagacacaa gataagggaa caacacaggt agtcaaagat ggcgaaactg
     2641 ccttcgtttt ggggcatgaa tttagtgtaa ttgccacacc gggtcacact ttaggacata
     2701 tctgttactt cagtaaacct tatctatttt gcggcgacac gctgttttct ggtgggtgtg
     2761 gtaggttgtt tgaagggaca gcatcacaaa tgtatcaatc acttaataag ttaagtgcgt
     2821 tacctgacga tacattggta tgttgtgctc atgaatatac gttatcaaat atgaagtttg
     2881 ctttgagtat tcttccgcac gatttgtcca taaatgatta ttatcgtaaa gttaaggagt
     2941 tacgggcaaa aaatcaaata acactacccg taattctgaa aaatgagcgg caaattaatg
     3001 tttttttaag aacggaagat attgatttaa ttaatgtaat taatgaagaa acattattgc
     3061 aacaacctga agagcgtttt gcatggttaa ggtcaaagaa agataggttc tgataaaact
     3121 ttcttgtcat cggctccgtt cgccgttatg atcggtcgtc ttttaagcaa ctattgacac
     3181 acacatgaag gcaaaagcga tattactcgc ctctgtcctg ctcgtgggtt gccagagtac
     3241 cggcaacgtt caacagcacg cacagagcct ttctgcagct ggtcaagggg aagcagcaaa
     3301 gtttacaagt caggcacgat ggatggacga tgggacgtct atcgcgccag atggtgactt
     3361 gtgggctttc attggcgacg agctaaagat gggaattccg gaaaatgacc ggattcgcga
     3421 acagaaacag aaatatttac gcaataagag ctatctccac gatgtaactt tacgggcaga
     3481 gccgtatatg tactggatag cagggcaagt taaaaaacgt aacatgccta tggaactggt
     3541 actactaccc atagtggaga gcgcttttga tcctcacgca acgtctggcg ccaatgccgc
     3601 aggcatctgg cagatcattc cgagcacggg gcgcaattat ggtttgaaac agacccgcaa
     3661 ttatgacgcg cgtcgcgatg ttgttgcttc aacaactgcc gcgctgaaca tgatgcagcg
     3721 tctgaacaag atgtttgacg gcgactggct tctgaccgta gcggcttata acagcggcga
     3781 aggtcgggtc atgaaggcaa ttaaaacgaa caaagcgcgt gggaaatcca cggacttctg
     3841 gtcgttaccg ttgccgcagg aaacgaagca gtacgtgcct aaaatgctgg cattgagtga
     3901 tattctcaaa aacagcaagc gttatggcgt acgtctgcca acgaccgatg aaagccgtgc
     3961 tctggcgcgt gtgcacctga gcagcccggt tgaaatggcg aaggttgcag atatggcggg
     4021 gatttccgtc agcaagctga agacattcaa cgctggcgta aaaggctcca cgctgggcgc
     4081 aagtggcccg cagtacgtga tggtgccaaa gaagcatgca gatcaactgc gtgattctct
     4141 cgcttcaggc gaaattgctg ctgtacagtc gacgctggtt gccgacaata cgccgcttaa
     4201 cagccgtgtt tacaccgtac gctctggcga cacgctttca agcatcgctt cacgtctcgg
     4261 cgtaagcacc aaagatttgc agcagtggaa caaactgcgc ggatctaagc tgaagccagg
     4321 ccaaagtttg acgattggtg caggcagtag cgcacagcgg ttggcaaaca acagcgatag
     4381 cattacgtat cgtgtgcgca aaggcgattc gctttcaagc attgctaaac gccacggcgt
     4441 gaacatcaaa gatgtgatgc gctggaacag tgacactgcg aatctgcaac caggcgataa
     4501 gctgacgttg tttgtgaaaa acaacaacat gccagattcc tgacaaacca gataataaaa
     4561 aaggcaccga ttcccccggt gcctttttta tttatgccgc tttatgcgct tctaccatga
     4621 tgatatcact ggtgaaagag ccatcagtct gcaaggaaaa atacgttctc acctctgtcg
     4681 atgcgctctg ttggtaaatg cgaatagcgt ctactaacgc ttctggcgta cgcattctca
     4741 cgacccatga agaaaattcc agcggtaact tatctgtaat taaattatca actataagat
     4801 tggcttcatt gattaacgtc aaccacgcac cgctggcgta gtttcgtacg tgagaggtat
     4861 cgcgtaatgc ttctaccgcc tgtaaccaga tgtcgcgcac tgggtgaccc ggagacatta
     4921 catccatcac aatcagccta ccgccaggtt tcaatatcct attcacttct cgcagtgctg
     4981 caccaacatc atgccagtga tgggcagaat aacggctgat aacaatatca aatgcgttat
     5041 cggcaaatgg cagacttgcg gcatatccct ggcgggtggt gatatttttc agttgccggg
     5101 cttcggcagc ttgtgccacg acatccagca tgtgggccga taagtcatac gccaccaccg
     5161 cgctcacgtt ttgcgcagcg ccaaacgttg gtctgccctg tggcagacct gacatacctt
     5221 atttatcagg cttgccggga ctgaattcaa cgagtagcgg attgtgatcg gaagcgcgcg
     5281 taaccagtac agaagcttca ctgacgttca gaccacggta gaaaacaaaa tcgagcgggc
     5341 gaccaaacgc ccgacggcgc tgatcatcgg taaaacgcac ctggcgcagc gacatttccc
     5401 gcgcaaagcg atataacgcg ttcatccttc tacggctcca ggcattgaaa tctcccgcca
     5461 taatgaccgg gccgctgtgg tgagctatct gatcaccaat aggaagtaac tgcttactat
     5521 agacatccac gcccagactg aagttgacgg cgtgtatatt aaccaccatc aacaggcggg
     5581 tgtcaggtaa tggatagacc gtcaccagtg ccgacttcgc cagacgcaaa atgggttctc
     5641 gttcacgtaa cgggcagcaa tacactggat gtgccgccga aagggtcatt acgccagaag
     5701 gatgttgtgg cagcacgaaa gcgggtacct gatcggcggc aagatagtta gcggtcgcaa
     5761 actgtactaa ctctggcgtt gtctgcgctt cctgcaataa caccaggtgt gcatctttgc
     5821 cgtagttctt taataccgac aaccattcag cgcgttgctg tttgtatatg ttccacacca
     5881 ggatccgaat acgctcttcg gtacttaacg gttccccagg tggtaatgcc tggccgatgc
     5941 tcgcaaaaga ccccggcggt aagatccttt ccgcaggttg tccggcaaca tagcgcatgg
     6001 cataggtgtt ttttcgcact tttgactttg aaacctctat tacgtcaacc cttccgcact
     6061 cgggagggtt ctactattat agggatttta gctcactctt tcaacgagca attgtatatt
     6121 gttatgtaag caagtgctta caccggtcag aattttgctc attatggaga acattaaatc
     6181 gcatgacata tcacgcatcc tgatggttta atgacaaata aattcaaaat atctcttcta
     6241 ttttgcaaca ggagcaaaaa tgaaagccac gtcggaagaa ctcgccattt ttgtttcggt
     6301 cgtcgaaagc ggcagcttta gccgggcagc ggaacaatta gggcaagcaa actcagcggt
     6361 aagccgggcg gtgaaaaagc tggagatgaa acttggcgtt agcctgctta atcggaccac
     6421 gcgacaactt agcctgacgg aagaaggcga gcgttatttt cgtcgcgtac agtcaatttt
     6481 gcaggagatg gcagcggcag aatcagaaat tatggagacg cgtaatacac cgcgtggact
     6541 gttacggatc gatgccgcaa ctccagtggt gctgcacttt ctgatgccgt taattaagcc
     6601 tttccgtgaa cgctatccgg aagtcacttt gtcgctagtc tcctccgaaa cgattattaa
     6661 tttgatcgaa agaaaagtgg atgtcgcgat acgcgctggt acgttaacgg attccagctt
     6721 acgtgccagg ccgttattta acagttatcg aaaaattatc gcctcccccg attatatttc
     6781 ccgctacggg aagccagaaa cgatcgacga tttaaagcaa catgtttgcc tgggattcac
     6841 tgaacccgct tccctcaata cctggccgat agcctgtagt gatggacaat tacatgaggt
     6901 gaaatacggt ttgtcatcca atagtgggga aacactgaaa cagctttgcc tgagtggcaa
     6961 cgggattgcg tgtttgtccg actacatgat cgacagagaa atcgctcgcg gagaattggt
     7021 ggagttaatg gcagataaag tgttgccagt ggaaatgcca ttcagtgccg tctattacag
     7081 cgaccgtgcg gtaagtacgc gcatccgggc ttttatcgat ttccttagcg agcatgtaaa
     7141 aacagctccc ggaggagctg tcagagaggc ttaatcccat tcaggagcca gaccttccgg
     7201 gctaaccagg cggtcgttgc aatccagtgc ggcgatcgct tttttatctt cggcatcaag
     7261 ctgtaaattt tgtgccttaa gattactttc caggttttta cgtttagtag aagaaggaat
     7321 tactgagtaa ccttccccca tagcccacgc cagaatcact tgtgccggag tcgcattgtg
     7381 tttagctgcg atacgagcaa taacctcatc tttcagggcc ttaccatacg ccagcgtcat
     7441 ataggaagta atatggatgc cgtgctgttt agcccaggca accactttac ggttttgcag
     7501 ataaggagag agttcaatct ggttagtagc gatgttttca gcgccaacag cagcaatcgc
     7561 cttttccatc aatgggatcg tgaagttgga aataccgatc tcacgcgtca gcccttgttt
     7621 tttggcttcc agcagcgcct gcataacccc ttcaacagag acttcatcgt ttggtgacgg
     7681 ccagtggatt agcgtcagat caacataatc ggtacgcaat ttttgcaggc tctctttcag
     7741 acttgggatc aatttgtctt tgctgagatt ttcaatccag attttagtgg tgatgtagag
     7801 ttcatgacgt ggcacgccac tttctgcaat cgcctgacct actgcggctt cgttatcata
     7861 gatttgtgcg gtatcaattg cgcgataacc aagttcaagc gccgttttca cagatgaaat
     7921 aacaacgtct aaaccaaatg cagggatagc cataatatgc ctcttttaat gctattttcg
     7981 ttaactgagg tgattatggg cttcagactg ttgaggaaaa aagcgtaaaa tgcagaggat
     8041 ttttgcgatt ctggcaataa tagatataca gacgaaaaaa aacctcgagc gttaactcga
     8101 ggcttcttaa taa
//
LOCUS       DADLFA010000174         7915 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000174 DADLFA010000000
VERSION     DADLFA010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7915
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.176"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            195..1028
                     /gene="ppsR"
                     /locus_tag="JQH65_003383"
     CDS             195..1028
                     /gene="ppsR"
                     /locus_tag="JQH65_003383"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HAY6140692.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRTANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            1184..2230
                     /gene="aroH"
                     /locus_tag="JQH65_003384"
     CDS             1184..2230
                     /gene="aroH"
                     /locus_tag="JQH65_003384"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HAY6140693.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            2362..2553
                     /gene="hemP"
                     /locus_tag="JQH65_003385"
     CDS             2362..2553
                     /gene="hemP"
                     /locus_tag="JQH65_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HAY6140694.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(2557..3993)
                     /locus_tag="JQH65_003386"
     CDS             complement(2557..3993)
                     /locus_tag="JQH65_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HAY6140695.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNIELANTLSIP
                     SSLFKNGAGVWGGEALLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TAEPGAMLMRVAPSHLRFGHFEHFYYRRESEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLIQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(4056..4769)
                     /gene="ydiV"
                     /locus_tag="JQH65_003387"
     CDS             complement(4056..4769)
                     /gene="ydiV"
                     /locus_tag="JQH65_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HAY6140696.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            complement(5015..5479)
                     /locus_tag="JQH65_003388"
     CDS             complement(5015..5479)
                     /locus_tag="JQH65_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase"
                     /protein_id="HAY6140697.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKQQASIGTQIDKDKLLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            complement(5557..6306)
                     /gene="btuD"
                     /locus_tag="JQH65_003389"
     CDS             complement(5557..6306)
                     /gene="btuD"
                     /locus_tag="JQH65_003389"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HAY6140698.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVGLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLILTI"
     gene            complement(6306..6857)
                     /gene="btuE"
                     /locus_tag="JQH65_003390"
     CDS             complement(6306..6857)
                     /gene="btuE"
                     /locus_tag="JQH65_003390"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HAY6140699.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            complement(6920..7900)
                     /gene="btuC"
                     /locus_tag="JQH65_003391"
     CDS             complement(6920..7900)
                     /gene="btuC"
                     /locus_tag="JQH65_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HAY6140700.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISLGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELLIGVVTATLGAPVFIWLLLKARR"
BASE COUNT         2057 a         2104 c         1844 g         1910 t
ORIGIN      
        1 gttgcgcaaa cctgctctat tcgacaattt ccacataaac catcggtata aacgaaacca
       61 taaaaatgaa atgctgtttt cataaaaaat aaaattgaag gttcatttta taaaccagac
      121 attacctcct acgcttctgt gcgtttttaa tttatgcttt catagaatta tgtctgcatc
      181 acgggaagaa caaaatggat aatgctgttg atcgccacgt tttttatatt tctgatggta
      241 cggcaataac tgcggaggta ttaggacacg cagtaatgtc acaatttccc gtcactatca
      301 gcagcattac gctgccgttt gtcgaaaatg agagccgtgc acgggcagtg aaggatcaga
      361 ttgacgcaat ttatcaccag acaggtgtgc gcccgctggt cttctactcc atcgtgttgc
      421 cggagattcg cgccatcatc ttgcaaagtg aaggcttttg ccaggatatc gttcaggcgc
      481 tggttgcccc gctacaacaa gagatgaaac tggatccaac gccgattgct catcgtaccc
      541 atggccttaa ccctaataat ctcaataaat atgatgcgcg cattgcggcg attgattaca
      601 cccttgccca cgatgacggc atttcgttgc gcaatctgga tcaggctcag gtgatcctgc
      661 tcggtgtttc tcgctgtggt aaaaccccca ccagtctgta tctggcaatg caatttggta
      721 tccgcacggc aaactacccc tttattgccg acgatatgga taatctggtg ctacccgcgt
      781 cgctcaaacc gcttcagcat aaattgttcg gcctgactat cgacccggaa cgtctggcgg
      841 cgattcgcga ggaacgtcgg gagaacagtc gctatgcctc gcttcgtcag tgcagaatgg
      901 aagtcgcgga agtggaagcc ttgtaccgta aaaatcagat cccgtggatt aacagtacca
      961 attattcggt agaagagatt gccaccaaga tcctcgatat catgggcctt agtcgccgaa
     1021 tgtactagag aactagtgcg ctggtttgtt ttttgttatc atgctaacca cccggcgagg
     1081 tgtgacacac ctcgcacttg aaatcagctg cgattggttt atcgtgatgc gcatcacttc
     1141 ccggtagtcc tgccgtagaa gcaacaaatt tctgagactt gtaatgaaca gaactgacga
     1201 actccgtact gcgcgtattg agagcctggt aacgcccgcc gaactcgcgc tacggtatcc
     1261 cgtaacgcct ggcgtcgcca cccatgtcac cgactcccgc cgcagaattg aaaaaatact
     1321 gaatggtgaa gataagcgac tgttggtcat tattggcccc tgctcgatcc acgatctcac
     1381 cgctgcaatg gagtacgcca cccgtctgca gtcgctgcgc aaccagtacc agtcacggct
     1441 ggaaatcgta atgcgcacct attttgaaaa accacgaact gttgtcggct ggaaagggct
     1501 aatctccgat ccggatttaa acggcagcta tcgggtaaat cacggtctgg agctggcgcg
     1561 caaattactt ttacaggtaa atgagctggg tgtccctacc gcgactgagt ttctcgatat
     1621 ggtgaccggt cagtttattg ctgatttaat cagttggggc gcgattggcg ctcgtactac
     1681 cgaaagtcag atccaccgcg aaatggcttc agcactctcc tgtccggtag gttttaaaaa
     1741 tggtaccgat ggcaacacgc ggattgccgt ggatgctatc cgcgcagccc gcgccagcca
     1801 tatgttcctc tcgccagaca aaaatggtca gatgaccatc tatcagacca gcggcaaccc
     1861 gtatggccac attattatgc gtggcggcaa aaaaccgaat tatcatgccg atgatatcgc
     1921 cgcagcctgc gatacgctgc acgagtttga tttacctgaa catctggtgg tggatttcag
     1981 ccacggtaac tgccagaagc agcaccgtcg ccagttagaa gtttgtgagg atatttgtca
     2041 gcaaatccgc aatggctcta cggcgattgc tggaattatg gcggaaagtt tcctgcgcga
     2101 aggaacgcaa aaaatcgtcg gcggtcagcc gctcacttac ggtcaatcca ttaccgaccc
     2161 gtgtctgggc tgggaagata ccgaacgcct ggtcgaaaaa ctcgcctctg cggtagatac
     2221 ccgcttctga atgcgtgccc attcctgatg ggatgggcat ttctgcgcaa cttgttgtct
     2281 tctcaacaca ttactgcttg ctctggcctg ccataatatt gataataaga atcattgtta
     2341 tatcaattat tattaatttt tatgcgttat acggatagca gaaaactcac gcctgaaacg
     2401 gatgccaatc acaagaccgc ttccccgcag cccatccggc gaattaccag ccagacactg
     2461 ttaggtccgg atggcaaact gattatcgat catgacgggc aagaatatct gctccgtaaa
     2521 actcaggctg gcaagctgct gttaactaag tagcctttaa ctcgagcagc tgacttccag
     2581 ccgtttaccc cagtcaggtg gacgactgac atagtcatca tctctgtcgc tgaaaggatt
     2641 tcgcaacgcc tcatgcaggc ggtgcaattc catcatatca cccttttctg ccgcttcaat
     2701 cgcccgttgc gccaaccagt tgcgcaatac cagagctggg ttaacgcttt gtatcagttg
     2761 ctgacgctca ctatcgctaa cctcgtcttg ttgcaaacgc ccccgataac gggcaaacca
     2821 gtcatcaaat gccgcacgat caataaactc atcacgtagc ggtgacgccg cgctgtgctg
     2881 ctcggtcaga ctcagcatgc ggaatgtgcg ggtataatcg ctgcgctctc gcgccatcag
     2941 actgaataat tcattcagta gcgcgttatc ctctttttgc tccgtcatga agcccagttt
     3001 ctgccgcatc cgttgtccat aatgcgtcaa caaaacctgc tgatagctgt ccagggcctc
     3061 attcagggca tctacggcaa caaatggtga caatgtctgc gccagacgct gtaaattcca
     3121 caacgcgaca gcaggttgat tatcaaagct ataacgccct tgatgatccg agtgattaca
     3181 aataaaaccg ggttcgtaat catcaagaaa accaaacggc ccgtaatcaa gcgtcagtcc
     3241 cagcagcgac atgttgtcgg tattcatcac cccatgtgca aagccgaccg tctgccattg
     3301 ggcaattaac gacgcggtac gtgcgacaac atcgctaaac cagagacggt atttgtcctc
     3361 atcatcttca agatgtgacc agtaatgacg aatagcaaag tcagctaact gacgaacctt
     3421 ttccgactcg cggcggtagt aaaaatgttc gaaatgacca aagcgcagat gacttggtgc
     3481 cacacgcatc agcatcgcgc ctggctccgc cgtttcccga tacactggcg aatcgctggt
     3541 gacgatactt aacgcgcggg tcgtcggaat gcccagataa tgcatcgcct cactggcgag
     3601 actttctcgt atcgtcgaac gtaaaaccgc ccgtccatca cccattcgcg aataaggcgt
     3661 caggccagca cctttcagat gccagtccat tgtagtgcca tcagcaagca gttgttcgcc
     3721 gagtaaaatg ccgcgcccat cacccagttg gcccgcccag acgccgaact gatgaccact
     3781 gtaaacctgc gccagtggtg acataccagg cagtagggct tcgccgcccc agacacctgc
     3841 gccattttta aacagcgacg atggaatact cagcgtgtta gccagttcga tattatgcca
     3901 aatcagccgg gcattattta aaggcgtagg ggaaagtgct gtataggttt ctggcaattc
     3961 atcgcgccag cgggtaacaa aagacagggt catagatcct cctgtttgat agtgtagacg
     4021 gttactctcg tcttaaacac cagcaaacag aagggttatc gctgaaccaa cgtcgttatc
     4081 tgatttatgg gaacggcagg ccacaggcat ccctggagag catggaaatc gaagggggta
     4141 atctgtgcca atatttctgc cgtatcaatg cctcccgcaa tgatgcaatt gcaacaaggg
     4201 gaaatttgcg cctggatcgc acgaataaac ggttcgaagg agcggtgcgt gatttgttgc
     4261 tgaataaaac ttttatccag catgacgcgg gtgaaaagac catcaaaaac cgctttcatt
     4321 gtgctattgc ctgctcccaa attacctaac acaaggggat acacttgcga taaggataaa
     4381 agaccgcgat tatcttttcc ttcattgaga tgcgggtaat tctcattaat caacagttca
     4441 ataaacggat attttaatag ttcgcctgcg taattatccc tctctaataa taatgtagcc
     4501 acttgtggcg ttaaattaag ccaggcaaag agtttatgtt gaataaaaaa atgttggcat
     4561 gattttagta attccaactg ctctgaaaat aattgccaat gttgctcctc ggtaagttgc
     4621 gctataaccc gactcgtcgg gatacgcacc gtgccatctt cactggagaa atgggtaatt
     4681 aactcaacgc cgacaaggac ttgctggtta tctcttatcg gcaggaaata gcaatcagaa
     4741 tgataaagat tctccaaaaa aatcttcatg gtaaccgtcc ctctcgaagg atgtttttca
     4801 gtatccggct aaaagggatg aggccataag acataacaat tacagaagga gtaactttca
     4861 tttgttccat gttaaccact ttttcagggg tcttttttta gattatcctg attataaacg
     4921 aataatccat ttacggaatt tttgtctgca aaatactact gtattcagga gtaaatggac
     4981 ggcgatgtat catcgccgtc catttttcga catactagat tcgtctcgcc tgccagaaat
     5041 ttttctgcca atagacatta tcaagtgagg aacgcatcac tcccttgctg gtagaggcgt
     5101 ggataaattg gttgttggta tcgtaaatac ctacatgcaa accattttgt ccggaccccg
     5161 ttttgaaaaa gaccaggtca cctggcagca acttgtcttt atcaatttgc gtgccaatag
     5221 aggcttgttg tttggtttca cggggcagct gcaaatcgaa acgatcgcgc atcgtcacaa
     5281 ccacaaatcc cgaacagtcc acaccgcgcc gcgtcatgcc accataacga tacggcgtgc
     5341 catgccagct ttgtagctgg tcgttcaaac cggcaataac ggtaatcgaa tcagaaagtc
     5401 tggcatttgg cggcggtgct ttatggtggc tacacccggc cagaaacagt gctgtgatca
     5461 aaataaggca gaaacgcatt ccgtacggtt cctctgtttt ttattcttgc attaatttag
     5521 cgtcgtaatt acccgatttt caagatacta atgaaatcag atggtcaaaa tcagcattct
     5581 gtgaccttcg atatccagac ggcgaaaatt catcccatag gcctgcgcca gatttggcgg
     5641 cgtgagcacc tcttctctgc gtccactggc cagcattttt ccacctttta gcaaccacgc
     5701 ccgatgcgca tgacgcaacg tgtggttgag atcgtgactg ctcatcacaa tcgccagtcc
     5761 ttgctgacac agcgcgctca gaattttgtc taacgcactt tgttgcgcaa catcaagact
     5821 gttcatcggc tcatcaagaa gcagcaattg gcctgcggga ttggcttgtg gtgtgatttg
     5881 caacaccacc gcagcaagac ctacgcgttg ccattcaccg ccggaaagtt gattggtgct
     5941 acgaccgagt ttgtcatcaa gagccagcgc ccctgcgaca tcattcagta gttcggtacg
     6001 cgttttatcg tgctgatgca gtgtcaggta gtgccagacc ggcattgcaa acggcggcgt
     6061 ctgctgttgt gaaagatagg cgcgatgcag cgcgagtttt gttgcggacc atgcttccag
     6121 gggttgcccc gcgaactgaa tgcttccctt accgctggtc attccggcca ttcgtgcaag
     6181 caaggtactc ttacccgcgc cattcggccc caccaggtgc aggatctccc cagcccgaac
     6241 ctcgccagaa agtggcccta ggcgggtaga ttccgcaaca tcctgtaact gcatcacaat
     6301 agacattatt ttgccaacgc cagtttaatg ctttccatca caatggggtc ttccggcgtc
     6361 atatccgggg aaaaacgctg gatgaccaat ccgtccctgc caactaaaaa tttctcaaaa
     6421 ttccataaaa tatcatcagg gtacagcggt gcacgacctt tgctgaccat acgggcatag
     6481 aatccgctct cttccggcgc gactgcggtc ggcgctgcgg caatcaattt ttgatacagc
     6541 ggatggcgtc cttcgccgtt aacttcaatc ttactgaaca tcgggaacgt caccccccat
     6601 gtggtggtac agtaagtttt aatctcttca tcgctgcccg gttcttgttc cagaaactgg
     6661 ttgcacggga atcccagcac cacaaaacct cgatcggccc aggctttctg aatattctcc
     6721 aactgctcat attgcggcgt taagccacac tttgaggcga cattgacaat caacagcaca
     6781 ttaccggcgt acttctccag cgtggtcact tcaccgtcga tatctttcac tacggtcgtc
     6841 agaatggaat cttgcatcgt ttctcctggg tgtggtcagt aaaaatctta gcttttaatc
     6901 atagaccgtc tttttgcggc taacgtcttg cttttaacaa taaccagata aacaccggcg
     6961 cacccaacgt tgcggtgacc acgccaataa gcagctctgc ggcagctaat gccaggcgcg
     7021 ctacaatatc ggccagcagc aatgcgctcg cccctgccag cgcgcagccg ggaagtaata
     7081 cgcgatgatc ggttaaacca cacaaccgaa gaatatgtgg gatcaccaga ccaataaagc
     7141 cgatagcacc cgccagcgcc acactgacgc caaccatcca gccggtcgcg gccaccagca
     7201 cattgcgcca gaaccacagg ggtaaaccca gttgccgcgc cgagatctcg ccaagtgcta
     7261 acatattcat cggcctggac tgacaacaga tccacaacaa cacggggatc aatgccagca
     7321 tcagccagct ttgccgccag tctacgccgc caaaaccgcc catcatccag tacatcagct
     7381 gacgtaaatc aacagaggtg gaaaagtaga tagcccacgt cattagtgcg ctacagataa
     7441 tccctaatgc aacgccagcc agcaataacc gactggtcga aagatgacga cgggcgaaac
     7501 gtaagagtat taaagtaatg ataagcgcgc cagcaatcgc acacagccct agcgcccagt
     7561 tggggagttg cccttgccca agcaataccg cggcgataag ccccacgcct gcgccgttag
     7621 agacgccaag tagtccaggt tctgccagag gattttcaaa caacgcctgc attacagcgc
     7681 cggatatagc cagcgccgca ccaaccaaca atacagccag cgtacgtggc aggcgaattt
     7741 gccagacgaa cagttcgcca cgaggagtaa accagtcacc tagcgagatc cattgttcac
     7801 cggcgcaaag gcttaaaaga agcgccagca gcatcaaaac tgacaggcat aataaccagc
     7861 gaatattttg tcgctgttgt tggcgggcaa gtgtcagcat ggtatccgtt ctgct
//
LOCUS       DADLFA010000175         7818 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000175 DADLFA010000000
VERSION     DADLFA010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7818
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.177"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            268..411
                     /locus_tag="JQH65_003392"
     CDS             268..411
                     /locus_tag="JQH65_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HAY6140701.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            424..633
                     /gene="cspE"
                     /locus_tag="JQH65_003393"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             424..633
                     /gene="cspE"
                     /locus_tag="JQH65_003393"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HAY6140702.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            799..1608
                     /gene="rlmA"
                     /locus_tag="JQH65_003394"
     CDS             799..1608
                     /gene="rlmA"
                     /locus_tag="JQH65_003394"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HAY6140703.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1605..2171)
                     /gene="mntP"
                     /locus_tag="JQH65_003395"
     CDS             complement(1605..2171)
                     /gene="mntP"
                     /locus_tag="JQH65_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HAY6140704.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(2599..3057)
                     /locus_tag="JQH65_003396"
     CDS             complement(2599..3057)
                     /locus_tag="JQH65_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HAY6140705.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDNLERIYKLIVLTQ"
     gene            complement(3112..3963)
                     /locus_tag="JQH65_003397"
     CDS             complement(3112..3963)
                     /locus_tag="JQH65_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HAY6140706.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(3976..4776)
                     /locus_tag="JQH65_003398"
     CDS             complement(3976..4776)
                     /locus_tag="JQH65_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAY6140707.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(4839..5810)
                     /gene="manX"
                     /locus_tag="JQH65_003399"
     CDS             complement(4839..5810)
                     /gene="manX"
                     /locus_tag="JQH65_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAY6140708.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            6273..>7818
                     /gene="yoaE"
                     /locus_tag="JQH65_003400"
     CDS             6273..>7818
                     /gene="yoaE"
                     /locus_tag="JQH65_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HAY6140709.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIVKE
                     QPAHDED"
BASE COUNT         1986 a         1913 c         1941 g         1978 t
ORIGIN      
        1 gcactatgta ataacttctc ccactggcct ggaacaactg aacttattga actatgttag
       61 aaaatacgcc agtttaagta tctgcctgaa ctggcaaggt taagcacaat gatatatcgg
      121 cgcgtattcc gttgcataag tgtgcaaaaa aagtggaaga cgtatcgaga tttgtgcgtc
      181 tgatcgagac atgtttaaaa atggcttgcc ataattaacg ttgtatgtga taacagattt
      241 cgggttaaac gaggtacagt tctgtttatg tgtggcattt tcagtaaaga agtcctgagt
      301 aaacacgttg atgttgaata ccgcttctct gccgagcctt atattggtgc ctcatgcagt
      361 aatgtgtcag ttttatctat gttatgcctg cgggcgaaga aaacaatcta aggaattttt
      421 caaatggcaa agattaaagg tcaggttaag tggttcaacg agtctaaagg ttttggcttc
      481 attactccgg ctgatggcag caaagacgtg ttcgtacact tctccgctat ccagggtaat
      541 ggcttcaaaa ctctggctga aggtcagaac gttgagttcg aaattcagga cggccagaaa
      601 ggtccggcag ctgttaacgt aacagctatc tgatcgaatc cactgatctg aagtgtgaat
      661 acgcttcaat ctcgctataa agcctcgtcg aatgcgaggc tttttactat gctttatctt
      721 cgctcctggc gttcggatat ttgcccgccg cgtgattcgc gttacacttg cggcctttag
      781 tatcctgccg gagttgtcat gtctttttcc tgtccacttt gccatcagcc tctttcgcgt
      841 gaaaaaaaca gctatatctg tccccagcga catcagtttg atatggcgaa agaagggtat
      901 gtcaatctgc tgcccgttca gcataaacgg tctcgtgatc cgggcgacag cgcggaaatg
      961 atgcaagcac gccgcgcatt cttagatgcc ggacattatc agccgctgcg tgatgcaatt
     1021 gtcgcccaac tgagggaacg gcttgatgat aaggccacgg cggtgctgga tattggctgt
     1081 ggtgaagggt attacacaca cgcatttgcc gatgcgttgc ccgaaatcac cacgtttggt
     1141 ctggatgttt cgaaggtagc gataaaagcg gcggcgaaac gctatccgca ggtcactttt
     1201 tgtgtcgctt ccagccaccg tttgccgttt tccgatacca gtatggacgc cataatacgt
     1261 atttacgcgc cgtgtaaagc agaagaatta gcacgagtag tgaagcccgg cggctgggtc
     1321 attactgcca cgccgggacc gcgacatttg atggagctga aggggctgat ttacaatgaa
     1381 gtacatcttc atgcacctca tgcagaacaa ctggaaggtt ttacattaca gcagagtgcg
     1441 gagttgtgtt atccgatgcg tcttcgcggt gatgaagccg tcgcattatt gcagatgacg
     1501 ccgtttgcct ggcgtgcgaa gccagaagtc tggcaaacac tggcagcaaa agaagtgttc
     1561 gactgccaga cggactttaa tattcacctc tggcagcgtt cttattaacc gtggaagtgc
     1621 gtccagagga tctggacgcc gatgccgatc agtaccagcc cgccgagaat ttccgctttt
     1681 ttcccaataa ttgagccgat aaagcgacca accatcatcc ctaatgttga cataatcaag
     1741 gttgcacaac caatggccaa tgcggtcgcg ataatgttga cctgcaggaa agcaagacca
     1801 acacccacag ccatggcatc caggctggtg gcaatcgcgg tggttaccag tagccagaaa
     1861 ccgtgtcgac ggcgcggctc ttcatcttca tcatctgcgc cacgaaaacc ctcaataatc
     1921 attcgcccgc cgaggaatat cagcagcaca aacgcaatcc agtggttcca ttcaaggaca
     1981 aaccggctgg ctaacatgcc cattccccag ccgatcagcg gcgtcagggt ttcgacggca
     2041 ccaaaaataa ggccggttcg caatgcttca gaaaatttcg gtttatggag ggtggcacct
     2101 ttaccgattg atgcagcaaa tgcatccatc gacataccaa acgcaagaag aacagtagca
     2161 gtgatattca tgacaatgtc ctgaccgggg tatccataaa acacatcatc gcccccagca
     2221 aacatgcata catgaacaac aagccgtgat ggtttttgcg ttcatgcagg catagcgatt
     2281 gatgtgcata tggtctcgcc tgactgaaag tattcagtcc gtacgtgcca cgttttgcaa
     2341 cgagtatgtt gacacgtaca tttccggaat ctggaaatcg gctactcccc aaatgagggc
     2401 gcaaccttaa catattttga gaatataaaa caactgttgg aatatattat ttcattgctt
     2461 tattgataac gaattcattt agatttatat atcaatgaaa cgttaaaata aataacaaat
     2521 aagggaagag gtaatatagc ctaagctatg tctgattatt aaaaatacac taaacatagc
     2581 tttggctaaa ttcataactt attgagttaa aacgataagc ttataaattc tttccagatt
     2641 gtcgatattt cgaacgcgga ttaacagccg acgctgttct aattgcatca ccaacacgcc
     2701 atcttccgac aagttcatcg ctttgattcg gctatattca atccagacat tggcgaagaa
     2761 aaaacctttt tgtttaaaga tgatcttcgg aacgcggatc cagaatatat aaaaacccat
     2821 cagagccagt gcgcttaata accatgtcgt tattaatgca ccatgattcg tgacgttgtt
     2881 atagataaga atgacaatca atccgacgaa gataacgcta tcgatgcgac cacgccggag
     2941 caaaggaatt gccagcaggg tggggccgtt acggcggggc atgatgaact gatcgtagat
     3001 cgcgaaggcc aggagtgcgg cgatgaaaag aatcagtacc aggtccgtga ttgtcattaa
     3061 tcctccagat aaaaaaacgg ggccaaaagg ccccggtagt gtacaacagt cttacagtcc
     3121 cagcaggccg caagcgtaac cagcgatacc gatgacgaag aagccaacga tgatccacag
     3181 cgggttaact tttttgcgca gtagccacat acaagcaaag gtcagcagca gtggtaccag
     3241 gcctggcatt aactggtcca gaatagtctg gacagtagta acgtgttctt tgcccgtctg
     3301 gtcagtaatg cgagagacaa ccagcgggat gttgacatgt gtccacttgt taaccaatgc
     3361 ccccatgaca aacaggccga ggatagacgc cccttccgtc agtttttgca ggaagccacc
     3421 acccatatct ttaacgatat cgataccttt ggagtaacca tacgctacgc cgtagtaacg
     3481 ggttgccaga cgcaccaggt taaacaggat gaagaacagc agcggaccta acaggctgcc
     3541 gctcatcgcg ataccggcac ccagtgctgc aaataccgga cgtacggttc cccagaagat
     3601 cgggtcgcct acaccagcca gcggccccat caaaccgact ttgataccgt tgatagcacc
     3661 gtcgtcgatc tctgcgccat tagcacgctg ttcttccagc gccagggtta cgccgagaat
     3721 cggcgcagcc acgaacggct gggtgttaaa gaactccagg tgacggcgaa tagcttgttt
     3781 acgagcttcg ttgttctcag ggtagaggcg acgaattgcc ggtaccatag agaagcagaa
     3841 acccagtgcc tgcatacgtt cgaagttcca tgaaccctgg aagaggttag aacgcaggaa
     3901 gacgccacga atatcacttt gagtgagttt tttctcggtg gtagtttgag ttgtatcaac
     3961 catttcgctc acctgttagt ccagttcgtt atcgagatcg ttgttaccag cagcctgagc
     4021 aggcgcaccg gctacgcggt tgtatttcgg gctaagttgg atgtagagca ctgccataac
     4081 agtaccgatc acacccagag caaccaggtt aaagttggtg aatgctgcgg ttacgaagcc
     4141 gaggtagaag aacggcatca ggtagccagc acgcatcatg ttgataacca tcgcataacc
     4201 aaccaccacg atcatgccac cggcgatatt cagaccattg gtcaccactt ccggaatcgc
     4261 attcagcatg ttctgtactt cgctggtacc aacagacagc gcaacgatga cggccggaat
     4321 agccacacgc attgcttgca ggaacagaga agaaacgtgg atccaggaaa tcgctgtcag
     4381 gttgccgtta tcagcagcct tatccgcagc gtgctggaaa gcaacggtaa tagtacgaac
     4441 gatgatggtc agtacctggc ccgcagcggc cagagggatt gccagtgcga tacctgcacc
     4501 aatgctctga tgacctgcga taaccagaat ggtagaaatg atagaagcca gagcggcgtc
     4561 aggcgcaact gcagcaccga tgttcatcca gcccagcgcg atcatttcca gcgtaccacc
     4621 gataataata ccggttttca tatccccaag aacgataccc accagggtac acgcgattag
     4681 cggacggtga aactgaaatt catcgaggat tgatcccata cctgcgatac aggctacgat
     4741 aaataccagc acaatttgaa gagtggtaat ctccattgta cttctcctgt ttacgtaaga
     4801 cttaagtgtg aaaactgagt gataatcaac acaatacgtt acttatcgat tttgctgatc
     4861 agatccatca ttttcagttt cggatcggtg gaaaccttac ggacttccag ctcaataccg
     4921 cgcgcattca gtttcttgaa cgcctcgata tctttttcat caaccgaaac cgcgttattc
     4981 acctgggttt taccctgacg gaatgccata ccaccgacgt taacagaggt gattttcacg
     5041 ccgccttcaa cgagacgctc tacatctgtt gggttggtaa ataacagcat tacgcgttcg
     5101 ccagcatatt tcgggttgtt gtagacgcga atcattttgg caacatcaac tacgtgtgct
     5161 gttacgcccg gaggtgcaac ctgggtgagc agtgtcttac gaacggtatc cgcagccact
     5221 tcatcactaa caacaataat acgggagaca ttggtttctt tggtccagcg ggtggcgacc
     5281 tgaccgtgaa tcagacggtc gtcgatacgc gcaaggccaa taaccatgta gtcgtttggc
     5341 cccattggtt ttgccggagt tggagccgct tttggtgctg cggcagcggg tgctggcgcg
     5401 gctttttcaa ccggtttggc tttcagtgct ttcacgcctt cacggcctgt ttctactgcc
     5461 agcgcaacca gttcatcaaa gcttgggtca tcatcacgag ccattaacgt ttccacgagc
     5521 attggaatgt taacgcctgc aatgacttca taatgctctt tgtcgacgac aatgcggctg
     5581 gcagcattga acgggctgcc tccccatgta tcaacgagaa acagcacgcc tttagtggtg
     5641 tcgagttttg ccaactgagc gttgtacttt tcaatcagcg tttcggcatt ttcacctgga
     5701 acgaaatcga tccagccgac gttttcctgc tcgcctaaca gcatttctgc cgttttaagc
     5761 aactgctctg cagcccaacc atgtgtgcct ataacaatag caatggtcac ttgctacctc
     5821 ctttattatc gttaacacct caacgtgcca gatgtatttt tgaatcgtcg tccacaatcg
     5881 aatcgattca gataagggct acaaccaaaa acccctgcgt ttcgcgattt attttagata
     5941 tcgaaaaaat tattttatgt gatgaagatc cgtaatttaa ctttcgatta gcagaaattt
     6001 cgaaaggtaa aatatccttg tcacattcgt ttgcaaagga aggtaaatct ttgccaaatc
     6061 agaggcgtct ctgatatgtt taactcccgt ttaacaacca tggagtatag ggcagtagcc
     6121 cgcagtatgg atcgtcaccg acgtcatttc agcatcaggc cttttaacgc ctgcctttct
     6181 ggcactctat gccgcacctt tcgtttgcat tttgtcgtta cgcctgcatt atttctggcg
     6241 tcgaatagct attccttaag caggagcttg tcatggaatt cttaatggac ccctcaattt
     6301 gggcggggct actcacgctt gttgttctcg aaattgtgct gggtatcgat aacctggtct
     6361 tcatcgccat tcttgctgac aaactgccgc caaaacaacg cgataaagcg cgtttgctgg
     6421 ggttatcact ggcgctgatt atgcgtctgg ggctgctgtc gctgatttca tggatggtca
     6481 cgctgaccaa accgctattt accgtcatgg atttctcctt ctccggacgc gacctgatta
     6541 tgttgttcgg ggggatattc ttgctgttca aagcaacaac cgaactgcat gaacggctgg
     6601 aaaaccgcga tcatgattcc ggccacggta aaggctacgc cagtttctgg gtggtcgtca
     6661 cacagattgt catccttgac gccgtcttct cgttggatgc ggtaattact gcagtaggga
     6721 tggttaacca tctgccggtg atgatggcgg cggtagtgat tgcgatggcg gttatgttgc
     6781 tggcatccaa accgctgacg cgattcgtta accagcaccc cacggtggtg gtgctctgtc
     6841 tgagcttcct gttaatgatt ggtcttagtc tggtggcaga aggtttcggt ttccacattc
     6901 cgaaaggtta cctgtatgcc gcgattggct tctcgatcat catcgaagtg tttaaccaga
     6961 ttgcgcgtcg caactttatt cgccaccagt cgactttgcc gctgcgagcg cgtactgccg
     7021 atgccatcct gcgtttgatg ggcgggaaac gtcaggccaa tgttcagcac gatgccgata
     7081 acccgatgcc gatcccggaa ggtgcatttg ccgaagaaga acgttacatg attaacggcg
     7141 tactgacgct ggcgtcgcgt tctctgcgcg ggatcatgac gccgcgcggt gaaataagct
     7201 gggttgacgc taatctcggg gtcgatgaaa tccgcgagca actgctctct tcaccgcaca
     7261 gtctgttccc ggtatgtcgc ggtgaactgg atgaaatcat cggtattgta cgtgctaaag
     7321 aactgctggt ggcgctggaa gagggcgttg atgtggcggc gattgcttcg gcgtctccgg
     7381 cgattatcgt cccggaaacc ctcgatccga tcaacctgtt gggcgtgctg cgtcgtgctc
     7441 gcgggagctt tgttatcgtg accaacgagt ttggtgtggt acaaggtctg gtcacgccgc
     7501 tggatgtgct ggaagccatt gcgggtgaat tcccggacgc tgacgaaacg ccggaaatca
     7561 ttactgatgg tgacggctgg ctggtaaaag gcggtacaga tttgcatgcc ttgcagcagg
     7621 cgcttgatgt tgagcacctt gccgatgacg atgatatcgc gacggtcgcg ggcctcgtga
     7681 tctcggcaaa tggtcacatt ccccgtgtgg gcgatgtgat tgatgtaggg ccactgcata
     7741 tcaccatcat tgaagccaat gattatcgtg ttgatctggt tcgcattgtt aaagagcaac
     7801 cggcgcacga tgaagatg
//
LOCUS       DADLFA010000176         7732 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000176 DADLFA010000000
VERSION     DADLFA010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7732
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.178"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(37..1032)
                     /gene="gapA"
                     /locus_tag="JQH65_003401"
     CDS             complement(37..1032)
                     /gene="gapA"
                     /locus_tag="JQH65_003401"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAY6140710.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            1374..1787
                     /gene="msrB"
                     /locus_tag="JQH65_003402"
     CDS             1374..1787
                     /gene="msrB"
                     /locus_tag="JQH65_003402"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAY6140711.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            1829..2101
                     /locus_tag="JQH65_003403"
     CDS             1829..2101
                     /locus_tag="JQH65_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HAY6140712.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(2262..2903)
                     /gene="pncA"
                     /locus_tag="JQH65_003404"
     CDS             complement(2262..2903)
                     /gene="pncA"
                     /locus_tag="JQH65_003404"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HAY6140713.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(2914..3930)
                     /gene="ansA"
                     /locus_tag="JQH65_003405"
     CDS             complement(2914..3930)
                     /gene="ansA"
                     /locus_tag="JQH65_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAY6140714.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPDDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(4097..5953)
                     /gene="sppA"
                     /locus_tag="JQH65_003406"
     CDS             complement(4097..5953)
                     /gene="sppA"
                     /locus_tag="JQH65_003406"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAY6140715.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDHNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPSAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            6114..6665
                     /locus_tag="JQH65_003407"
     CDS             6114..6665
                     /locus_tag="JQH65_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HAY6140716.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            6782..>7732
                     /gene="selD"
                     /locus_tag="JQH65_003408"
     CDS             6782..>7732
                     /gene="selD"
                     /locus_tag="JQH65_003408"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HAY6140717.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEV"
BASE COUNT         1944 a         1912 c         2000 g         1876 t
ORIGIN      
        1 gaagtcgctc tttttagatc acagtttcat ctcaacttat ttggagatgt gagcgatcag
       61 gtccagaact ttgttggagt aaccggtttc gttgtcgtac caggatacca gtttcacgaa
      121 gttgtcgttc agagcgatac cagctttagc atcgaacacg gaagtgcaaa cttcgccgtt
      181 gaaatcggta gatactacgt catcttcggt gtagcccaga acgcctttca tttcgccttc
      241 agcagcggct ttaacggcag ctttgatctg ctcgtaagtt gcagcttttt ccagacgaac
      301 ggtcaggtca actacggata cgttcggggt cggaacgcgg aacgccatac cagtcagttt
      361 gccattcagt tctggcagta ctttacctac agctttagca gcaccggtag aggacgggat
      421 tatgttctgg gaagcgccgc ggccgccgcg ccagtctttg tgagacgggc catcaacggt
      481 tttctgagta gcggtagtag cgtgaacggt ggtcatcaga ccttcgatga tgccgaagtt
      541 atcgttgata actttagcca gcggagccag gcagttggtg gtgcaggaag cgttggaaac
      601 gatgtcctgg ccagcatatt tgtcgaagtt agcgccttta acgaacatcg gagtgttgtc
      661 tttagacgga ccagtcataa ccactttctt cgcaccagcg gtgatgtgtt tacgagcagt
      721 ttcgtcagtc aggaacagac cagttgcttc agcgacaacg tcaacaccaa cttcgtccca
      781 tttcaggtta gccggatcac gttcagcggt aacacggatt tttttaccgt taacgatcag
      841 atgaccgtct ttcacttcaa cggtaccgtc gaaacggccg tgagtggagt catatttcag
      901 catgtatgcc atgtaatcag cgtctaacag gtcgttgatt gcaacgatct cgatgtcaga
      961 acgtttctga gcagcacgga aaacaatgcg accgatacgg ccaaaaccgt tgatacctac
     1021 tttgatagtc atatattcca ccagctattt gttagtgaat aaaaggttgc ctgtaaaatt
     1081 acaaaaacct tacgcagcgt caagcggaat cgtgtcaatc attgcgacaa atcaatcctg
     1141 tgcctaagca ttatgcgact gactcgcctc actcttcctt tgggcttgaa accacatggg
     1201 gtcggcgccc cgaattttaa agggcaatta cgataaaaat gtgattttca tcacgattta
     1261 ggtgcagcca tttcgtcgag atcaagttta ggacaaaatt ttgccagttg gtgttaatgt
     1321 tttgttagaa tcagtcacgt aatgtgagca cgattaaagt gagatgtgag caaatggcta
     1381 ataaaccttc ggcagaagaa ctgaaaaaaa atttgtccga gatgcagttt tacgtgacgc
     1441 agaatcatgg gacagagccg ccatttacgg ggcgtttact gcataacaag cgtgacggcg
     1501 tatatcactg tttgatctgc gatgccccgc tgtttcattc cgaaaccaag tatgattccg
     1561 gctgtggctg gcccagtttc tacgaaccgg tgagtgaaga atccattcgt tatatcaaag
     1621 acttgtcaca tggaatgcag cgcatagaaa ttcgttgcgg taactgtgat gcccatctgg
     1681 ggcatgtctt tcccgacggg ccgcaaccaa caggcgaacg ttattgtgtt aactctgctt
     1741 ctttacgctt cactgatggc gaaaacggcg aagaaatcaa cggttgaaaa aacgattcag
     1801 caacttattc cacaggagcg gaaaaattat gaatcttgat gacattatca acagcatgac
     1861 gcctgaggta taccagcgtt tgtcgaccgc cgttgaactg gggaaatggc ctgatggcgt
     1921 tgcgttgacc gaggaacaaa aagagaattg cctgcaactg gtgatgctgt ggcaagcccg
     1981 ccataatacc gaagcacagc atatgacaat tgacacaaat ggtcagatgg tcatgaagag
     2041 caaacagcag ttaaaagaag attttggtat ctcagcaaag cctattgcta tgtttaagtg
     2101 aatgagttat ctagtattta agcctgttat taagggaggg caaatatact tgttcggacg
     2161 tcgtgtttta gctgcaagta ggttaaatgc aacactgaca agtattatct ggcctacaat
     2221 gtcattgagg aaatgtaggc cagagatgaa ttcaactaat attacccctg tgtctcttcc
     2281 cagtctgcca gcgtatatag cgttgcccca gctgctgaca tctccataaa cgcgtgtgca
     2341 ctgtcctggg gctggatatt cacgccacga caaccatcgg taatcacgtt caccttataa
     2401 cctaactgta acgcgtccag cacggtaaac ttcacgcaat agtcagtagc caggcccata
     2461 acgatcaatt catcgatttc atgatcgcgt aaccagtcat cgagagaggt tttctgccga
     2521 cggccgttat caaaaaaggc actgtaactg tcaactaaag gattttcgcc tttatggaac
     2581 accgctgcga tcgctttttg gttcagtaac ggatgtaatt gtgcgccttc actgttctgc
     2641 acacagtgat ctggccagaa ggtttgtggc aaaccgtcga gttggcctgg cgtataaggc
     2701 tctacaccgt gctgactggc aaaactgccg tgattcgccg gatgccagtc ctggctggcg
     2761 ataaccgctt ctccgcgcga ctggcaccag tcaataaggc ggttagcaac ctccaccgta
     2821 ctgtcacctt ccggtacggc gagcgcgcca ccagcacaga aatcattttg taaatcgacc
     2881 agtaacaggg cgcgatgggg cattgcagtc tccttaatca tccggcgtca gttcgccgcg
     2941 caggttttgg ctcatggcct tgcgaatggt ttcagtatcc agttcctggc tcagcaggta
     3001 atgcagtttg gttagcgtgg cttctacagt catatctgca ccgccaataa cgccggcatg
     3061 ggcgagggcg ttaccggtgg cataaccacc catattcact ttaccggaca tacattgtgt
     3121 caggttgacc accacaatac cgcgatcgct ggcttcttgt aattcctgca ggaaggcttt
     3181 gttttgtggc gcattaccca cgccatagga gcgcagaatc aatgctttca ctggttggcg
     3241 cagaaaattg cgcacgacat cagcagaaat ccccggataa atcgtcacta cgccaattgg
     3301 ttgtggcgtg attggatgaa cgattaattc cccttcaccg tgcggggcgg gtggcgtatt
     3361 caaacgacga atatggatac cagcttccag taacggagga aggtttggag aggcaaacgc
     3421 atcaaaacca tcggcatggg ctttggtagt gcggttgccg cgatacaatc ggttattgaa
     3481 aaagagcgtt acttcgttga tcggataatt cgccgcaacg tacaacgcat tcagcagatt
     3541 aatttgtccg tcagagcgta actcagccag cgggatttgt gaccctgtca caatgaccgg
     3601 tttaccgaga ttctcgagca tgaacgacag cgcagaggcg gtatacgcca tcgtgtcggt
     3661 accgtgtaga atgacaaaac catcatagtc gtcatagtgc gctttaatat cttcagcaat
     3721 atgctgccag tcatccggcg tcatatcaga agaatccatc aacggcgtat attcatgaat
     3781 ggtgaaatct ggcatctccg ggcgatggaa ttccggcatc agcgccagtt ggcgttgtag
     3841 atgacctgac accggaatat aaccctgctc ggaacgctgc atcccgatgg tcccgcccgt
     3901 gtaggcgacg taaattgatt tcttttgcat gatattgata tgaatgatat cgaaagagca
     3961 aaagtatata cgtgaggcag gaggagcgca cctgatacta tgcggataag gaaatatggg
     4021 gcgagacagc gggaagagga ttcgtcgagg taaaaaataa tgtggccgac gatctgtcgg
     4081 ccactcagta caagacttaa cgcacgttgg cgcaggtcag gcaaaacgca taacggtttt
     4141 gtgggtcgtt aaacgcggcc agcttgtcgc tttcactttt aacggtagag gctaccgagg
     4201 caagcggtgc aggtaacatg gcctggaacg catctggcaa cattgcccgg acagaaccag
     4261 acatgttgtc catcactttg tcgaagaagg tcggttcatc aacgtagtat tccagatgcc
     4321 actgtttcac ttttgcgagc tctgctgctt tggcaaccgc atcatcgaaa tccccgagac
     4381 tatcgaccag cccgttagct tttgcatcct gaccggtcca gacgtggccc tgggcgattt
     4441 tatcaatttg ctccggcgtc gaatgacgcg catcagcaac cagcgtgata aagcgtttat
     4501 agccattctc aatgcttaat tgcatcatct gctgcgcttc cggcggcagt gccctggtga
     4561 tagaaacatc cgccagcggt gaagttgaga caccatcagt atgaacacca atcgaatcca
     4621 gactattttc tacggtggtg atcacgccga agataccgat agaaccggtc agggtgctgg
     4681 ggttagccac aatgtaatta gctggcgtgg aaatccagta accaccagat gccgccatgc
     4741 cgcccatcga tacaaccaca ggcttacccg ctgcccgggc tgctgccagt tcagcgcgaa
     4801 tcacttcaga cgcggtaacg ctgccgcctg ggctattaac acgcaggaca atcgctttca
     4861 ctttcgggtc aaggcgagcg tcgcggattt gtgccgcagt ggtatcaccg ccaacattcc
     4921 cctgagtttc ctcgccatcc ataattgcgc cattagcgaa gacgacaccg atgctgtcac
     4981 cggtatctgc cggcgttttc aatgcgtaat cgtaataact gatggcgcga taatttttat
     5041 cagtcttact ccagccgaac tctttggtca gagctttttc gatttcggca ctcgatgcca
     5101 gtgcatcgac cagcttgttt tccagtgcat atttcgcggt atcgccaccg gttttggtta
     5161 aaccctcaag caacccttgc gcgctaggga atacctgctg agcagggatc tgccggttag
     5221 cggcaacagt attcagatag ttttgccaca gctcaccaat ccagcggctg tcagcttcgc
     5281 gggctgccgg tgacatatca tcacgaataa acggttcaac ggcagattta tacgtaccca
     5341 cgcggaacac atgggtggaa actttcagct tatccagcaa cgatttgtag tacagaccgt
     5401 tggtagcaaa accgtgcaga tcaaccacgc cttgcggtga cagccaaatt ttattggcga
     5461 aactggcgag ataatattgc ccctggctgt agttctcgcc aacggcataa accggtttcc
     5521 cgctgtcacg aaactctttc agggctttgc cgatgtactg catagacggc tggtcgccgc
     5581 ctgcgaagtt tttcagatcc atcacgatac cggtgatatt gtggtcgtcc ttcgcctggc
     5641 gaatggtgtt gacgatatca aacagtgagt tttcctgcag acgatcggaa ctggcaccaa
     5701 gcagctggcg gcttaattta ctaaaccgct gagaactgtc gggtttatcg acgatcacac
     5761 cggaaatgtc cagcagcagt gcgccacgac tggccgtttc tttcgaatca ccaccactga
     5821 cctgcatcca gatccccaca ccaaccagta cgaggaaaat aaagaacagg ttaagtacca
     5881 tttcacggac gaaattcagc agacgccacg tccatttaaa aaatccggca ataaatcgcc
     5941 aaagggttcg catgtattct cccaacttaa ggtcacacct gtcacaggcg caatagatgt
     6001 ggttatcgta atgacccaac cgacacttgt cagcaggaat tgcggactgt gctgtaacaa
     6061 aatccactct cgtgttaatt ttgtgaataa atatcacgac aggagttaat caaatggatg
     6121 cactcgaact attgatcaat cgccgtagcg cctcccgcct ggctgaaccc gcgccaacgg
     6181 gtgaacaact gcaaaacatc ctgcgtgcgg gtatgcgtgc gccggaccat aagtccatgc
     6241 aaccgtggca tttttttgtg attgaagggg aagggcgcga gcgtttcagc gccgtactgg
     6301 aacagggggc gattgctgcc ggtagtgatg acaaagctat cgacaaagcc cgtaatgcgc
     6361 cgttccgcgc accgctcatc atcacggtgg tggcgaaatg cgaagaaaat cataaagtcc
     6421 cgcgatggga acaggaaatg tctgccggat gcgcggtcat ggcgatgcaa atggcagccg
     6481 ttgcccaggg atttggcggc atctggcgca gtggcgcatt aactgaaagt ccggtagtgc
     6541 gtgaagcatt cggttgccgt gagcaggata aaattgtcgg ttttctctac ctcggcacgc
     6601 cgcagttgaa agcatctacg tcgattaacg tcccggaccc gacgccgttt gtaacttatt
     6661 tctgataatt gtcgcgaaac tgtctggatt gtgagcaaag gcgcaggaat tcagacactc
     6721 tcacttatca cttcacggaa tgagggttac catacccgga ttgcattgac aggagatgtc
     6781 catgagcgag aactcgattc gtttgaccca atacagccac ggagctggtt gcggctgtaa
     6841 aatttcccca aaagtgttgg aaaccatcct gcacagtgag caggcgaagt ttgttgatcc
     6901 gaatttgctt gtgggtaatg agacccgcga cgatgcggcg gtgtacgatc tgggcaatgg
     6961 caccagcgtt atcagtacca ccgacttctt tatgccgatc gttgataatc ctttcgattt
     7021 tggccgcatt gcggcgacta acgccatcag cgatatcttc gcgatggggg gcaaaccgat
     7081 tatggcgatt gcgatcctcg gctggccgat taacaaactt tccccggaaa ttgcccgcga
     7141 agtaaccgaa ggtggacgct atgcatgccg tcaggcgggt attgcgctgg ctggcggtca
     7201 ctccatcgat gcgccggagc cgatttttgg tctggcggta acggggatcg taccgaccga
     7261 gcgggtgaag aaaaacagca ccgcacaagc cggatgcaaa ctgttcctga cgaaaccgct
     7321 ggggatcggc gttcttacca cggctgagaa aaaatcactg ctgaaaccag aacatcaggg
     7381 actagcgacg gaagtgatgt gccggatgaa catcgcaggc gcgtcctttg ccaacatcga
     7441 aggcgtaaaa gcgatgaccg acgttacggg ctttggtctg ctgggccatt tgagcgaaat
     7501 gtgtcagggc gcaggtgtgc aggcgcgcgt tgactatgac gcgatcccaa aattgccggg
     7561 ggttgaagag tacattaagt tgggcgcagt gcctggcggc accgaacgta actttgccag
     7621 ctacggtcat ctgatgggtg aaatgccgcg tgaagtgcgc gatttgctgt gcgatccgca
     7681 aacttctggc ggtttgctgc tggcggtcat gccggaagca gaaaatgagg tc
//
LOCUS       DADLFA010000177         7643 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000177 DADLFA010000000
VERSION     DADLFA010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7643
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.179"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            566..3979
                     /locus_tag="JQH65_003409"
     CDS             566..3979
                     /locus_tag="JQH65_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAY6140718.1"
                     /translation="MEQSLNFEMLRSQWPELAELACMAERYVHSDPESCLVKLRNYTE
                     LMVRWLYRQERLPEGIKANLYDLMNADVFTSMMPEAIIMKMDALRIHGNRAAHGGRIK
                     AKDTYWLLKEAYLLGIWLYVRYAHGNVDDCPKFTLPPLTQSSGRADEKRLEDAIKAQD
                     ESRERELALQRALQQEQEKAEHLTQRLNEARARNQHVADILSIDEAETRRRLIDSRLL
                     AADWNVGEELKNTDQVTQEHPVKEQPTTSGDGYADYVLWDEAHKPLAVVEAKKTSVNA
                     EQGRIQARLYADWLEKEYGQRPVIFYTNGYDIWLWDDHKTHGYPPRRVFGFYSKESLQ
                     YLIQQRETRLPLNRVPHVKDNEGKAVAGRLYQLETIARVSERFTNKYRQSLIVQATGT
                     GKTRVAIALSKLMIDARWVKRVLFLCDRKELRKQAANAFNQFTNEPLYVVGKSKKADR
                     QNARIYIATYPGMMKIMDHFDVGYFDLIIADESHRSIYNVYGDLFKYFDALQIGLTAT
                     PIDMVSKTTFGLFGCEGRIPTANYSLEDAIADNNLVPYEVVTHTTEFLREGIKREKLS
                     DAQIRELEEQGIDPNTLEFDGKALDEAIYNKDTNRYILRNLMENGLKDRDGQLPGKTI
                     IFARNHKHALLLNELFDDMYPQFAGRFCQVIDNYDPRAEQLIDDFKGLDESTNKELTI
                     AISVDMLDTGIDVPEIVNLVFAKPVKSKVKFWQMIGRGTRLCPGLYGYDDNGKPLDKQ
                     KFRIFDHWGNFEYHELHTEEAEVTATKSLAQKRFEAWVMLGAAAQRKFDKQAVDLVAH
                     QLREQINALDEKSIAVQEKWQQKAQYSDEKVLRQLSPKTQQDLLSVLAPLMQWLDVRG
                     QSDAIRFDMDILAAQTARYTNPEELDVLWPIIVEKVERLPPHLAQVQQQGQRINQLRN
                     LSWWKQASLEELEDIRIHLRGIMHLMEKDATPKFGSIQVDITEDANLIQTETRKTNIR
                     SIDFKLYRQQVQGALEPLFQQNPVLKKIRNGEPVTQNELDELAKLVLIQNPNVDIRAL
                     KEFYPQATASLDKLLRTIIGMDSDAVEVRFAQFAADNSLTSQQLRFLSLLKNHIRDYG
                     TIEMRQLFEQPFTHIHNEGVTGVFPDIAQIARLQKIVEELGVVTDAATV"
     gene            4043..5677
                     /locus_tag="JQH65_003410"
     CDS             4043..5677
                     /locus_tag="JQH65_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HAY6140719.1"
                     /translation="MITGDLKSKIDGLWEDFWVGGITNPLTVIEQITYLMYSRMLDTQ
                     EQRDEKRKQIAGIDFKPRFAPEQQEFRFSHYSNLGSDEMMEVVRDGVFQHFRQLGQAD
                     ASKVTLLGNFMKDARLEIVKPSLLTKAVEVIKNLPLDRGDTKGDLYEYLLSKLTTAGI
                     NGQFRTPRHIIRTMVEMMEPNPARGETICDPACGTGGFLATSYEYLLEKYSSLESIHT
                     EIGTNERGELEEQKIFTGDLLTPWRNHVDNNMFHGYDFDTTMLRIAAMNLIMHGVDAP
                     DIHYQDTMSQSFSTNFPQASKNAFNLILANPPFTGSLDEEDIDSTLSAMVKTKKTELL
                     FLARILQMLKVGGRSATIVPQGVLFGSSKAHQSLRKTLVEDNQLETVINLPSGVFKPY
                     AGVATAILIFTKGGQTDEVWFYDLQNDGYSLDDKRNPIKDNDLPHLLASWKHYRTLRG
                     LPVDNFMGKKLASLLKQQYPEGINAGVDFKDRTQAAFVVPKADIAAQKYDLSINRYKE
                     VVYQAEEYEDPKVILKRLKDLEKEILADLDELEGML"
     gene            5686..6756
                     /locus_tag="JQH65_003411"
     CDS             5686..6756
                     /locus_tag="JQH65_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAY6140720.1"
                     /translation="MVKLGDVINVHYGKALKADQRVSNGSVHVFGSSGIVGNHDKTLC
                     SYPTIIIGRKGSVGAITWAPSGGWIIDTAYYVEIKDNNKLDLRYLFYILSGIDLTKKT
                     ITTSIPGLNRDDLYDTFIKLPPFEEQKRIVDLLDKAEGIRQKREQSIKLADDFLRATF
                     ATMYGNPITNPKKWPVHLMGEIIEFKGGNQPPKSDFIFEPKQGYIRLVQIRDFKSDKY
                     ATYIPQEKAKRIFEVDDVMIARYGPPVFQILRGLSGSYNVALMKASPKENIRKGFIFY
                     LLQLPEYHDVVVKNSERTAGQTGVNLELLNKFNVPLPPIYYQDEILDRLARIEKFKEK
                     IEISLNHLEIQFLSLQKRLIGF"
     gene            complement(6881..7597)
                     /locus_tag="JQH65_003412"
     CDS             complement(6881..7597)
                     /locus_tag="JQH65_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6140721.1"
                     /translation="MLSETKKRIPAHLFRMIKDELYELVLTDDPKGKLYVRDSEKIEE
                     GVSTTEFVVGYGAISMVKKSESMATKGLVGLERVDLIREVVFENGYYDWGCVVNDVLP
                     NICKGNARIPIFHFLNHAKLINPDGSIINETELSGGVLSRLNITPISFQSQGWDKRRS
                     ETVPEVKVGVNELYLAYDFSFFLRMMPYMEPGLIKRDMGELLKILKKHIDEAMNTQVL
                     SSNFCKLVCIYDYIKNSHRY"
BASE COUNT         2223 a         1740 c         1793 g         1887 t
ORIGIN      
        1 tcctgttctc ccaaatatca tggtattgat acgggagatt atgccgtaca ggtacgacac
       61 gttatgtcgt gccaaagggg agtggataga aaaaatggcg aagatgcgaa aagttgatta
      121 atgcattgaa aaaaaaatga attaataatg aacttgggta aaagcgccat caaaatacga
      181 cacgacctgt cgtattttgt gttagccggg aaaagatatc tgctacttac taaaatgatt
      241 tagtgctgaa aatatttata agccaataaa tagacaggta ttacggagta acgcccaggc
      301 tattttaacg agcctgctgt tttattggct caaaaaacat atcccataga tgatcagcca
      361 tacccgcgtt agtgagcaag gtatcccttg agtagccagg gctgaagacg cccattgatg
      421 ggcgaaaacc aaggttctgg ccgttaaagc ggctatgtcg ttaaacacat caccaccata
      481 tgccatccgg ctcacttcgt cggccaaatt agtgtgatat tctttccaga atttctgttg
      541 tatccacaac ataaggcgat gattgatgga gcagtctctc aattttgaaa tgttgcgcag
      601 ccagtggccg gaactggcag aactcgcatg tatggcagag cgctatgttc actccgatcc
      661 ggaaagctgc ctggttaagc tgcgcaacta caccgaattg atggtgcgct ggttgtatcg
      721 tcaggagcgg ttgccggaag gtattaaggc taatctttac gatttaatga acgctgatgt
      781 ctttaccagc atgatgccgg aagccatcat catgaaaatg gatgcgctgc gtatccatgg
      841 caaccgtgcc gcgcacggcg gacgtatcaa agctaaagat acttactggc tgctcaaaga
      901 agcgtatttg ttgggaattt ggctgtatgt tcgctacgcc cacggtaatg ttgatgactg
      961 ccccaaattt acactccctc cattaacaca atcttcaggt cgtgcagatg aaaaacgtct
     1021 ggaagatgca atcaaggctc aggatgaaag ccgtgagcgc gaactggcgc tacaacgcgc
     1081 actacagcaa gaacaggaaa aggccgaaca cctcactcaa cgcctgaatg aagccagagc
     1141 gcgtaaccag catgttgccg atattctctc tattgatgaa gcggaaaccc gccgtcgcct
     1201 gattgactct cgcttacttg cagccgactg gaatgtaggg gaagaactta aaaacacgga
     1261 tcaagtcaca caagaacatc cagtcaaaga gcaacccacc acctctggtg acggttatgc
     1321 agactatgtc ttgtgggatg aggcacacaa accgttggcg gtggtggaag caaaaaaaac
     1381 cagcgtcaat gccgagcaag gacgaattca ggcccggctg tatgcagact ggctggaaaa
     1441 agaatacggt cagcgtccgg tcatcttcta caccaacggc tatgatatct ggctgtggga
     1501 tgaccataaa actcatggtt atcccccgcg tcgggtgttt ggcttctaca gcaaggaaag
     1561 cctgcaatat cttattcagc agcgtgaaac ccgtctgccg ctgaacagag tgccacacgt
     1621 aaaagataac gaaggtaagg ccgttgccgg acgtttgtac cagctcgaaa ccattgcccg
     1681 cgtcagtgaa cggtttacca acaagtaccg acaatcgtta attgttcagg ctacgggtac
     1741 aggcaaaacc cgcgtggcaa ttgcactaag caaactgatg attgatgccc gctgggtaaa
     1801 acgcgtcctg tttctttgcg accgtaaaga actgcgtaaa caggcggcaa atgcctttaa
     1861 tcaattcacc aatgagccgc tgtatgtggt cgggaaatcg aaaaaagcag acaggcagaa
     1921 tgccagaatc tacatcgcta cctatcccgg tatgatgaaa atcatggacc atttcgatgt
     1981 cggttatttc gatttgatca tcgccgatga atcccaccgc tctatctaca acgtctacgg
     2041 cgatctgttt aagtattttg atgccctcca gattggcctg acggccacgc caattgacat
     2101 ggtgagtaaa accactttcg gcctgtttgg ttgtgaagga cgtattccta ccgccaacta
     2161 cagcctggaa gatgccatcg ccgataacaa tctggttccc tatgaggttg tcacacacac
     2221 aacagaattc ctgcgcgaag gtatcaaacg ggagaaatta agcgacgcac aaatccgcga
     2281 gctggaagaa caaggcatag atcccaatac gctggagttt gatggcaagg cgctggatga
     2341 ggcgatctac aacaaagaca ccaaccgcta tatcctgcgt aacctgatgg aaaatggcct
     2401 gaaagatcgg gacggccagt tgcctggtaa aaccatcatt ttcgcccgta accacaaaca
     2461 cgcgctgctg ttgaatgaat tgttcgacga tatgtaccca cagtttgccg ggcgcttctg
     2521 ccaggtcatc gacaactacg atccccgcgc cgagcaactg attgatgatt ttaaagggct
     2581 ggatgaaagc accaacaaag agctgaccat cgctatctcc gttgacatgc tcgataccgg
     2641 tattgatgtc ccggaaattg tgaatctggt ctttgccaaa ccggtcaaat caaaagtgaa
     2701 gttctggcag atgattggcc gtggtacgcg cctctgtccg gggttgtacg gttacgacga
     2761 taacggcaaa ccgctggata aacagaaatt ccgcattttc gatcactggg gcaattttga
     2821 gtatcacgag ctgcataccg aagaggcgga agtcacagcg acaaaatcgc ttgcgcaaaa
     2881 acgcttcgaa gcgtgggtta tgctgggggc cgctgcgcag cgtaaattcg acaaacaggc
     2941 ggtggattta gttgctcacc agcttcgcga gcaaatcaac gcactggatg aaaagtcgat
     3001 tgccgtacag gaaaaatggc agcagaaagc gcagtacagt gatgaaaagg tgctgcgcca
     3061 gctttctccg aaaacacagc aggatctgct atctgttctg gccccgttga tgcagtggct
     3121 ggacgtgcgc gggcaaagcg atgccatacg ctttgatatg gatattctgg cggcgcaaac
     3181 tgcccgttat accaacccgg aagagctgga tgttctctgg ccgatcatcg tcgaaaaagt
     3241 ggagcgcctg ccgccgcatt tggcgcaggt gcagcaacag ggacaacgaa tcaatcagct
     3301 tcgtaattta agctggtgga agcaggccag cctggaagag ctggaggata ttcgcattca
     3361 tttgcgcggt atcatgcacc tgatggaaaa agacgcgacg cctaaatttg gttcgataca
     3421 ggtggatatt accgaagatg cgaacctgat ccagacggag acccgcaaaa ctaatatccg
     3481 ctcgattgat ttcaaactct atcgccagca ggttcagggg gcgctggagc cgctgttcca
     3541 gcaaaacccg gttctgaaga aaatccgcaa cggcgagcca gtcacgcaaa acgagctgga
     3601 tgagctggcg aagctggtgc tgatccagaa ccccaacgtt gatattcgtg cgttgaaaga
     3661 gttttatccg caggcaaccg ccagcctgga taaacttttg cgtaccatca tcgggatgga
     3721 cagcgacgcg gtggaagtgc gttttgccca gttcgccgct gataacagcc tgaccagcca
     3781 acaactgcgc ttcctgtcat tgctgaaaaa ccacattcgc gattacggca ccattgaaat
     3841 gcggcagctc tttgaacagc cttttacgca tatccacaac gaaggcgtta ccggtgtgtt
     3901 ccctgatata gcgcaaatcg cccgcctgca aaagatagtc gaagagctgg gtgttgtgac
     3961 cgacgcagcg acggtataat acacgcatga caagccccgg cagcggggct tttgtattta
     4021 aaacaggata taaacgctaa caatgattac tggcgactta aaaagtaaaa tcgacggact
     4081 atgggaagat ttctgggtgg gtggtatcac caacccgctg accgtaatcg aacagatcac
     4141 ctatctgatg tattcccgga tgctggatac ccaggaacaa cgcgacgaaa agcgtaaaca
     4201 aatcgcgggt attgatttta aaccgcgttt tgcgccagaa cagcaggagt tccgtttcag
     4261 tcactatagc aaccttggct cggatgagat gatggaagtg gtgcgcgatg gcgtattcca
     4321 gcatttccgt cagctcggcc aggccgatgc ttcgaaggtg acgctgctgg gcaactttat
     4381 gaaagatgct cgtctggaga ttgttaagcc gtcattgctg accaaagcgg ttgaggtaat
     4441 caaaaacctg ccattggatc gcggcgacac caaaggcgac ctttacgaat acctgttaag
     4501 caagctgaca actgccggaa tcaacggaca gttccgcaca ccgcgccaca ttatccgcac
     4561 gatggttgaa atgatggagc cgaacccggc ccgcggcgag acgatttgcg atcccgcctg
     4621 tggcaccggt ggttttctgg caaccagcta tgaatatctg ctggagaagt acagctcgct
     4681 ggaatccatt catactgaga ttggcaccaa cgaacgtggc gagctggaag agcaaaaaat
     4741 ctttaccggc gatctgctga caccgtggcg taaccatgtg gataacaaca tgttccacgg
     4801 ttacgacttt gacaccacaa tgctacgtat cgccgccatg aacctgatta tgcacggcgt
     4861 ggatgcgcct gatatccact atcaggacac aatgagccag agtttcagca caaacttccc
     4921 gcaggccagt aaaaacgcct tcaacctgat tctggcgaac ccgccgttta ccggttcact
     4981 ggacgaggaa gatatcgact ccacgctgtc ggcaatggtg aaaaccaaaa aaaccgaact
     5041 actgttcctg gcgcgtattc tgcaaatgct gaaagtgggc gggcgcagtg ccactatcgt
     5101 gccgcagggc gtgctgtttg gctctagcaa ggcgcaccag tcacttcgca aaacgctggt
     5161 ggaagataac caactggaaa cggtgatcaa tctgccttct ggtgtattta aaccttacgc
     5221 tggcgtggcg acggcgatcc tgatctttac caaaggcggt caaacggatg aggtctggtt
     5281 ctacgatcta caaaatgacg gctacagcct ggatgataag cgcaacccga taaaagacaa
     5341 cgatctgccg catctgctgg caagctggaa gcattaccgt actttacgcg ggctaccggt
     5401 tgataacttt atgggtaaga agttagcctc gttgcttaaa cagcagtacc cggaagggat
     5461 taatgctggc gttgatttta aagatcgcac gcaggcggcg tttgttgtac cgaaagcgga
     5521 tattgctgcg cagaaatacg atctatccat caaccgttat aaagaagtcg tgtatcaggc
     5581 ggaggaatat gaagatccga aggtgatatt gaagcggtta aaggatctgg aaaaagagat
     5641 tctggcggat ttggatgagc tggaggggat gctgtgagtt cggcgatggt gaagttaggg
     5701 gatgttatta atgttcacta cggaaaagca ttaaaagctg atcagcgtgt atcgaatgga
     5761 agtgtccatg tgtttggtag ttcaggaatt gtcggcaacc acgataaaac gctttgcagc
     5821 tatcccacga taattattgg ccgcaaaggc agcgttggtg ctattacctg ggctccaagc
     5881 ggaggctgga tcattgatac tgcttattac gttgagatca aagataataa taagttagat
     5941 ctccgatact tgttttatat tctgagcgga attgatctca ctaagaaaac gattaccaca
     6001 agtattcctg gattaaatag agacgatctc tatgatacat ttattaaatt accacctttt
     6061 gaagagcaga aacgaattgt ggatcttctc gataaagctg agggtatacg ccaaaaacgt
     6121 gagcaatcca tcaaactggc tgatgatttt ttgcgtgcta cgtttgctac aatgtatggg
     6181 aatcctatta caaaccccaa aaaatggcct gtccatctta tgggagaaat tattgaattt
     6241 aaaggtggaa atcaaccacc taaaagtgat tttatttttg aacctaaaca gggttatatt
     6301 agattagtac aaattagaga ttttaaatcc gataaatatg cgacgtacat tccgcaagaa
     6361 aaagcaaaac gtattttcga agttgatgat gttatgattg ctcgttatgg gccacctgtg
     6421 tttcaaatac ttagaggttt atccggcagc tacaatgtag ctttaatgaa agcttcaccc
     6481 aaagaaaata ttaggaaggg gtttatattc tatcttctac aacttccgga atatcatgat
     6541 gttgttgtaa aaaactctga aagaacagca ggacaaaccg gtgtaaatct tgaactatta
     6601 aacaaattca atgttcccct cccacctata tactatcaag atgagatatt agatagatta
     6661 gctagaattg aaaaatttaa agaaaaaata gaaatatcat taaatcattt agaaatacaa
     6721 tttttatcac tacagaagag attaataggt ttttaaatcc ttagtatcga aataaacatt
     6781 aatttattat attaaattaa gatattttag gaaattaagg cgtcactctt aggtaacgcc
     6841 tttttttact atctcgcccc ctaagaaggt tatatcttac ttaatacctg tgactatttt
     6901 tgatataatc ataaatacac actaatttgc aaaaattact tgacaacacc tgagtattca
     6961 tagcttcatc tatatgtttt ttcaatattt tgagaagctc tcccatatcg cgcttgatta
     7021 atccaggctc catataaggc atcattctta aaaagaaact gaaatcataa gcaagatata
     7081 actcattgac ccccaccttg acctcaggaa cggtttcaga gcgtcgttta tcccatcctt
     7141 gagactgaaa actaatagga gtgatattta atcgtgataa aacgccacct gagagttctg
     7201 tttcattaat tatactccca tcaggattaa ttaatttagc gtgatttaag aagtgaaaaa
     7261 taggaatgcg cgcattgcct ttgcatatat ttggcaaaac atcattaaca acacatcccc
     7321 aatcataata tccattttca aatacaacct ctcggatgag atctacacgc tccagtccaa
     7381 ccaatccttt agttgccatc gattccgatt tttttaccat cgaaatagca ccatatccca
     7441 caacaaactc agttgtactg accccttctt caattttctc tgaatccctc acatataatt
     7501 ttcctttcgg atcatcagtt aagaccaact cataaagttc atcttttatc attcggaata
     7561 aatgtgctgg aatccttttt ttagtttcac tcagcacctc aaatatttct ttataggatg
     7621 gtgcctgaac atattttacc ggg
//
LOCUS       DADLFA010000178         7625 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000178 DADLFA010000000
VERSION     DADLFA010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7625
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.180"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..311)
                     /locus_tag="JQH65_003413"
     CDS             complement(<1..311)
                     /locus_tag="JQH65_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HAY6140722.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     V"
     gene            complement(305..1543)
                     /locus_tag="JQH65_003414"
     CDS             complement(305..1543)
                     /locus_tag="JQH65_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAY6140723.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAQPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVTMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFTSARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTMVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(1940..2179)
                     /locus_tag="JQH65_003415"
     CDS             complement(1940..2179)
                     /locus_tag="JQH65_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HAY6140724.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(2182..2901)
                     /gene="sanA"
                     /locus_tag="JQH65_003416"
     CDS             complement(2182..2901)
                     /gene="sanA"
                     /locus_tag="JQH65_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HAY6140725.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(3051..3935)
                     /gene="cdd"
                     /locus_tag="JQH65_003417"
     CDS             complement(3051..3935)
                     /gene="cdd"
                     /locus_tag="JQH65_003417"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAY6140726.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(4065..4760)
                     /locus_tag="JQH65_003418"
     CDS             complement(4065..4760)
                     /locus_tag="JQH65_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HAY6140727.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMHIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(4757..5155)
                     /locus_tag="JQH65_003419"
     CDS             complement(4757..5155)
                     /locus_tag="JQH65_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAY6140728.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            5394..6344
                     /gene="dusC"
                     /locus_tag="JQH65_003420"
                     /pseudo
     CDS             5394..6344
                     /gene="dusC"
                     /locus_tag="JQH65_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311941.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
     gene            complement(6732..6815)
                     /gene="yohP"
                     /locus_tag="JQH65_003421"
     CDS             complement(6732..6815)
                     /gene="yohP"
                     /locus_tag="JQH65_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HAY6140729.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            6949..>7625
                     /locus_tag="JQH65_003422"
     CDS             6949..>7625
                     /locus_tag="JQH65_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311052.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HAY6140730.1"
                     /translation="MLLMYCHAKLKNISQHTVISAHRFLPDYSPMNRDSFYPAIACFP
                     LLLMLAGCAPMHETRQALSQQTPAAQVDTALPTALKNGWPDSQWWLEYHDNQLTSLIN
                     NALQNAPDMQVAEQRIQLAEAQAKAVATQDGPQIDFSADMERQKMSAEGLMGPFALND
                     PAAGTTGPWYTNGTFGLTAGWHLDIWGKNRAEVTARLGTVKARAAEREQTRQLLAGSV
                     ARLYWEW"
BASE COUNT         1966 a         1960 c         1909 g         1790 t
ORIGIN      
        1 aatactttgt ccgggtaatc ttctttcagc cgacgcaggg cggccagatt ctcctccagc
       61 ggatgctcgg caatctgctc catatttttg aagccgataa aaccggtatc ttctttcacc
      121 agatgatcaa aacgcggcga gacttcgttg gcgataaaaa agccgatcgt tttaaacacc
      181 acaccgcccc aacctgtgtc gtaggctttg gcacacatct catagcagtt gcctaccggc
      241 gaagaagaga ggcagaacgg gttgggaaac ttcacgccgc aaaaagtaat cgaaagatct
      301 ttcgttaaca tgagcaagct ccctctaaat agtgatgaat cgcctcggcg gcttctttcc
      361 cggttttcac ggcatagacc atggttttgt caccctcaac aatatcgcca gcagcaaaga
      421 cttgcgggtc gcgggtctgg taattttgtg ttttgatggt gttacgctgc ggctctaact
      481 ccgcaaaggc atctagtctg gcatgctgac cgacggcgag aataatttta tctgccgcca
      541 tcgtcagttc gcccgataac cgtacatgct taaacgtgac tttattgcct tccacggcta
      601 ctggcgtgaa tccatcgatg atcgaaacgc ccagctcccg ggcgctggta aactcttttt
      661 cgcttgccgg aaactcatct aactcttcac gcgctacgca agttaccgcc tgacagccga
      721 gaactttcag cgtgctggct acgtccatcg tgacatcacc gccgccgata attaatgcgc
      781 tttgtggaat gctgatatcg ccttgcgcct gtcgtgcacg ttgcaagaag tcgacggcaa
      841 tctcaacgtc actatgctca aacagcggta gcccggaacc gcttgataac cccacggtga
      901 ccagtaccgc gcggttttct gctttcagct gctcaagggt gagtgtgtta ccgacttcgt
      961 tgttgcactt aatggtcacg cccatttttt caatacgggc gatctctgca tccagtactg
     1021 attgcggtaa ccggaattgc ggaatgccgt tacgcaacca gccaccgggt tgtgcttctt
     1081 tctcatagat cgtgacgtca taaccctggt ttgtcagtgt cacactggcc tgcaatccgg
     1141 caggacctgc gccaataatc gcgactttgc cgagcgtttt agtaccgggc tgataaattt
     1201 ccatcccggt ttgttgttca aaatcagtaa caaaacgctg taagcggccg atatcaatgg
     1261 gagcatcaac accggcacgg gtacaaccgc tttggcataa tttctccgtc gggcacactc
     1321 tggcacaaac ggcaccgagg gcattatttt cgcgaattgt ctcggcagcg cctttaaaat
     1381 tacgaaagta gattgagcga ataaatttcc ccggatcggt ctgtgccggg caagcctgac
     1441 tacagggagc gtcgtgacat aataaacagc gagaggcttc tttaatcgcc agtaacggcg
     1501 taaaagccgg agtgagttca tccagataat tttgttgcgg catgtgatct tccttaatgc
     1561 tatgagtaat taaggcgaac gctaaatata ctcgtcatac ttcaagttgc atgtgctgcg
     1621 tctgcgggtt attcggccca tccatgggcc tcacccctta tggggccgct gcaggcagcg
     1681 ttcaaatccg ctccatgcgg atttgtcgct caccccagtc acttacttat gtaagctcct
     1741 ggggactcac tcacttgtcg ccttcctgca actcgaatta tttagagtat aaatattgcg
     1801 tgctgatatt aaggctatgc gcgtggggaa atttatactg tgaaaggatt cacaaatagc
     1861 tgcggttcag aatgttatat ccgcagctta ttgagtgaag taataattat tctcaataat
     1921 taacagaaaa gagaaaatat taacgataga aaataagata aacaatcatg ccgatagata
     1981 aaattccgaa gccgatatat gccaccatat aagcgaaaaa gagtccggga ttttttgctc
     2041 gccagacata caccggttcc ggtgcatttt taacccgttt atcaatcgcg cctgcgcgcc
     2101 agcctttcca tgcttcgcgg aaacaaaata tcggaataag gaaaaaaacg gcaacgacaa
     2161 aaaactgctg tacatccata actactttcc ttgtttcttt tgtaattcaa gtaactgttc
     2221 gggtgtgacg gcgggatacc cctgcgcatc ttccggcacc tggtgcatag ccggaatagg
     2281 gaccagcggc cctaaaaaac gcggttcacg tttaaaaata taaaggtcag ccagcgcacc
     2341 gaaacgggcg gcaaattcgc gaatacgtac tgacagcata tctttcggtg acggtacggc
     2401 ataacactga gcctgaatcc ccatatgcag cgcaataaat aatgcccgct cacagtggaa
     2461 acgttgggtg ataataatga aatcattagt atcgaaaact ttgcgtgtac gcacgatgga
     2521 atccagcgta cgaaagcctg cgtaatcgag aacaatatct gatgggtcga caccagcagc
     2581 gattaaatct ttgcgcatgg tcatcggctc attataactt tgcaatgcgt tatcgccgct
     2641 cagtaataga taatttacct taccgctgtt ataggcatta atcgctcctt gaatgcggta
     2701 gcgataatac tgattaatta cgccagtacg ataatatttt gctgttccga gcaccacacc
     2761 gacctggcgg taggggagat cctgcaattc gtcgtagata taaggcgcgg ttttccagct
     2821 catccagcga tcgaggccga gcacagtcaa caacagcaag ccgatcagga ctaacaggct
     2881 gaggaacacg cgctttaaca tgcaggagac tcgatctaag gaaaatttcc atcaggctac
     2941 tttacccgct ggttttgcgc aagaaacggc aaactgtttg ccgggaattt cgacaccagg
     3001 agagggctgg caagccggac aaggcgttca cgccgcatcc ggcaccaggc ttaagcgaga
     3061 agcactcggt cgatactgtg acagccgaga gctttcagcg ttgcggaggt ggcatcccac
     3121 tgaatcaacg gcgcatcggc tttttctgcc agaaccgcgc gctggatatc cgggtaatca
     3181 taacccttga gattcaacag aattaacgct ccctgcaacg gtggcagagt cgggttgaat
     3241 gcggcgtttt cagcgtagct gccactgaaa atacgaccgt ctttacattc cagcgcgaca
     3301 ccgcttggcg acttactgta aggcatgtgc gaacggtttg ccgctgcaat cgccgcctga
     3361 gaaagcgcat caccagtcag cgcatagccg tgatcctgct cgtccatcag cagcgtttta
     3421 atctccagat ctttcggccc aaaggcatcc ggcagatagt cacgcagcgc gtgtgcctcg
     3481 cggcccggca gatgaatacg cagatccaga ccgctgttca gttcattcat aaactgacgg
     3541 cagtgaccac aaggcgtgta gttaacggtg atggctgcaa gcgctttttc accactcaac
     3601 caggcgtggc tgatcgcgct ttgttcggca tgaacggttt gctgcattgt cgcaccaata
     3661 aattccatat tggcaccgaa ataccaggtt ccgctcacac cgcgcgcaat tgcgccaaca
     3721 ttaaaattcg acaatggcgt acgcgcacag gccgctgcca gcggaagtag tgcgaatgcc
     3781 agcgcgtctt cgtccagccc cgttgcgctc ttcagcgatg agacttgctc cccggtcaac
     3841 aaagcgggga agtacttgtc tgccagaata ggttccagtg cagattgcaa gttatccgca
     3901 agttgggcaa aagcggtttg aaaacgtgga tgcatggcgt tgcctcataa tcatgtaatg
     3961 ggataacagt ttacgaaccc gttatggctt tatatgtgat ctgaatctca ttaattatgc
     4021 aacgcgtgaa acttacatca aaaatgcgcg acgcatcgca aattttagcc cattactgcc
     4081 agaataatcg ggaaaaggaa cggtgcgatc agcgaagtaa ttatcccgca taacaccagc
     4141 gctagcgaac taaatgcacc ttcctgataa tccagctcgg cgcagcgcgc cgtaccgagg
     4201 gcgtgcgagg cagtccccat tgccagaccg cgcgcagctt tggtacgaat atgcatcgca
     4261 ttaagcaatg tatggccaaa taccgcgccg aggatgccga cgaaaatcac gcaaactgcg
     4321 ctgattgccg gaataccgcc aatactgccg ccaaccgcca ttgcaatagg cgtggtgact
     4381 gatttcggca ggattgacgc ggcgatttcc ggtgaagcgc ccatcaacaa tgccacggaa
     4441 gtccccgtca ccatcgcaac cacgctgccg ataaaacaga tggtgatgat cgatttccag
     4501 cgcgcgcgga tctggtgtag ctgctcatat aaaggatagg ccagcgcgac gaccgccggt
     4561 tgcagcagat cgtttagcac ttcgctacct ttaaagtagc tgtcgtaaga gatgccggtc
     4621 agcattaaaa aagggatgat caccaccatt gctaccagca acgggttaag caagggaaac
     4681 ttataccgtg ccgccagttt gcgggcggca aaaaagacga tcaatgtcag tggtaatgac
     4741 caccagatat tagccatcat tcttctgatc ctttctgacc tacgacttta cgttcaccgt
     4801 gcaccagttg cgaactccag ctcaccacca gaaaaaccac cagtgtactg acagcacagg
     4861 aaaccactac cgggccaaac tgtgcgcgga gcaaatcaaa atattgcatg acgcctacgc
     4921 caatcggcac aaacaatagc gccatatagc gaatcagtac gtagcacccc ggattgaccc
     4981 attttgccgg aagaatttgc aaggccagca ggacaaacag gatcagcatc ccgatgatgc
     5041 tgcccggaat ggttaccggt agcagggaag caatgaaaat gcctgcatac aggcaggcat
     5101 aaatcaggac gaaagcgcgt aaatattgcc agataatgtt cagtgtctta ctcataatca
     5161 acaccccaga tgagatgaat tcatcataca attaattcgt aaaacgtgct accgatcaca
     5221 ttaagaaatc agcgcttatt ttttcgctcc ccggcattac gcaatgcgca acgaactcag
     5281 gcatacatca agcatagaga gggattacag tttgcagcaa tccctctttg cagaccttta
     5341 atccgttgac cataggcgct aacatagcgc ctcatttttt tgcgggtgaa gatatgcgtg
     5401 tgttactggc tccgatggag ggtgtgcttg actctctggt gcgtgaattg ctgaccgaag
     5461 ttaacgacta cgatctgtgc atcaccgagt ttgtccgcgt ggtggatcaa ctgctgccgg
     5521 taaaagtttt tcatcgcatt tgccctgagc tacaaaacgc cagccggaca ccatccggta
     5581 cgctggtgcg cgtgcagttg ttgggtcagt tcccacaatg gctggcagag aatgccgccc
     5641 gtgcggtgga attaggttcc tggggcgtgg atctcaattg cggctgcccg tcgaaaacgg
     5701 tcaacggtag cggtggtggg gcgacgttac tcaaagatcc tgaactcatc tactagggtg
     5761 caaaagcgat gcgtgaagct gtaccggcgc atttgcccgt cagcgtgaaa gtgcgtctgg
     5821 gctgggacag cggtgagaag aaatttgaaa tcgccgatgc ggttcaacag gctggtgcta
     5881 cggagctggt ggtgcatgga cggacaaaag agcagggtta ccgcgcggag catattgact
     5941 ggcaggcgat tggcgagatt cgccagcggc tgaatattcc ggtgattgcc aacggtgaaa
     6001 tctgggactg gcagagtgcg caacaatgta tggcgatcag cggctgcgac gcagtgatga
     6061 ttggtcgcgg ggcgctcaat attcccaacc tgagccgggt ggtaaaatat aacgaaccgc
     6121 gaatgccgtg gccggaggtg gttgctttgc tgcaaaaata tacccgtctg gaaaagcagg
     6181 gcgataccgg gttatatcac gttgcgcgga ttaaacagtg gttgagttat ttgcgtaaag
     6241 aatacgatga agcaacggaa ttatttcagc atgttcgggt gttgaataat tcccctgata
     6301 ttgcaagggc tattcaggca attgatatcg ataagctgcg ataatagtgg aatcgcagtt
     6361 tatcagagac aatgaatcgc ttattcagct tcatcatctt gttgcgctcc tttcatgagc
     6421 taagccacat attgccactg gcgcaaggag cgcgcagggg gaggacaatc gcctcctccc
     6481 cctgcccccc cgggctctgg cgaacaaaat cgccgctgcg cggtaccttc agcttatccc
     6541 cttcggctac cgggtcgggc gcgaggtaac gtccttgtaa gacgcgccct cagcccacat
     6601 ccatgtgggc tgccccggcc ttcggggaac gcttcagcga ttttgacgcc accaacaccg
     6661 gagctgtttg caaatcaagt acacaaaata tctagttcct gacctgacaa cttatatgcc
     6721 atatctgatt tttaaaatat catcttaaat acgccagtca ccaccagtag cccaatcagg
     6781 aaaataatca atacagccca gagtataatt ttcattggcg ttcctttatt tgtgagtgtt
     6841 gaataagtat aggaaaaaac gaagcccttt gccgttatcc ataaacgatt aagataaatc
     6901 ggaagatatc attcacttag tctatatttt tctgggtatg tcgttttatt gctactgatg
     6961 tattgccacg cgaaactaaa aaatatatcg caacacacgg taatctccgc gcaccgtttc
     7021 ttacctgatt attcccccat gaatcgtgat tccttttatc cagccatcgc ctgttttccg
     7081 ctgttattga tgctggccgg gtgtgcgcct atgcatgaaa cccgccaggc gttaagccag
     7141 caaacgcccg ctgcacaagt tgacaccgca ttacccacgg cgctgaaaaa tggttggcca
     7201 gacagccaat ggtggctgga gtatcacgat aatcagctca cttcattaat taacaatgca
     7261 ctacagaacg caccggatat gcaggtggct gaacagcgca ttcagctagc tgaagcccag
     7321 gcgaaggcag ttgccactca ggatggtccg cagatcgact tttcggcgga tatggagcgg
     7381 caaaaaatgt cggcagaagg cttaatgggg ccgtttgctc tgaacgatcc ggccgcaggt
     7441 acgaccggcc cgtggtacac caacggtact tttggcttaa cggcgggctg gcatctcgat
     7501 atctggggaa agaatcgggc ggaggttact gcccgcctgg gtacggttaa agcacgggcg
     7561 gcggaacgcg agcaaacccg ccaattgctg gctggcagcg tagcccgcct gtactgggag
     7621 tggca
//
LOCUS       DADLFA010000179         7595 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000179 DADLFA010000000
VERSION     DADLFA010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7595
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.181"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            97..735
                     /gene="ytfB"
                     /locus_tag="JQH65_003423"
     CDS             97..735
                     /gene="ytfB"
                     /locus_tag="JQH65_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HAY6140731.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            complement(719..1366)
                     /locus_tag="JQH65_003424"
     CDS             complement(719..1366)
                     /locus_tag="JQH65_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY6140732.1"
                     /translation="MYLDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIKVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWNYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKHNIFKPDIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            1578..2369
                     /locus_tag="JQH65_003425"
     CDS             1578..2369
                     /locus_tag="JQH65_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HAY6140733.1"
                     /translation="MKSTRPVAVITGAARGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLECLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            2379..3221
                     /locus_tag="JQH65_003426"
     CDS             2379..3221
                     /locus_tag="JQH65_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAY6140734.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLVPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINSTLDISVDRI
                     AHYC"
     gene            3231..4007
                     /locus_tag="JQH65_003427"
     CDS             3231..4007
                     /locus_tag="JQH65_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HAY6140735.1"
                     /translation="MSDQPVLFSRVAASCRLTLNREDKCHAINEEMIESLDHYLNEIE
                     NDTTLRLVELTATGDKFFCAGGDIKSWSAYSPLDMGRKWIKRGNDVFNRLRNIPQLTV
                     ANLNGHTIGGGIELALCCDIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
                     QMLMLGKRLTAQEAQAANLIDEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILAL
                     ENKHADYPHQLLAGLMSATQDCQQATRAFAEKSSVSFHNQ"
     gene            4017..5558
                     /locus_tag="JQH65_003428"
     CDS             4017..5558
                     /locus_tag="JQH65_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HAY6140736.1"
                     /translation="MRKITTAEALAAQIQDGATIAISGNGGGMVEADHILAAIEARFL
                     QTGHPRDLTLIHSLGIGDRDCKGTNRFAHAEMLKRIIAGHFTWSPKMQALVKNNTIEA
                     YCFPGGVIQALLREIGSGRPGLFTHVGLGSFVDPRNGGGKSNECTTDDLVELIEIDGK
                     TKLRYRPFKVDYAILRGTYADPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGAIEPRRVKLPGILVDGIVEHRKQPQTYLGGYDLTISGQHRRLSSNDAIELVSHP
                     VRRLIARRAARELVAGASTNFGFGIPGGVPGVALREGVPYQSLWLSVEQGVHNGMMLD
                     DAFFGCARNADAIIPSLDQFEFYSGGGIDITFLGMGEMDQYGNVNVSHLNGNLIGPGG
                     FLEIAQNARKVVFCGTFDAKGSKIDITPDGLHIAQSGQIPKLVTKVEKITFSAAYAQQ
                     SGQEVLYITERAVFQLTAEGVELIEIAPGVEIERDILPYMAFRPIIKHPRLMESSLFT
                     PMEDA"
     gene            5555..7595
                     /locus_tag="JQH65_003429"
                     /pseudo
     CDS             5555..7595
                     /locus_tag="JQH65_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005766390.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
BASE COUNT         1882 a         1893 c         2008 g         1812 t
ORIGIN      
        1 tatgaatata ataaaggcgg gtttaccacg gacatcatag cgttagctta ttcataggct
       61 atgattgagg aacaagacgc ggagcaggag gaaaccatgc ccgggcgctt tgaactaaaa
      121 ccaaccctgg agaaagtctg gcacgcgccg gacaattttc gcttcatgga cccgctgccg
      181 ccgatgcatc gtcgggggat cattattgcc gccattgtat tggtggtcgg atttctgctt
      241 ccatctgatg atacgcccaa cgcgccggtg gtgacgcgtg aagcgcagtt agatattcag
      301 tcgcaatcac aaccgccgac agaagaacag ctgcgggcgc aattagtcac accacaaaat
      361 gatcctgatc aggtggctcc ggtcgctcca gaacctatac aggaaggcca gccagaagag
      421 cagccgcaaa ctacccaaac gcaaccattc cagccagata gcggaattga taatcaatgg
      481 cgctcttacc gtgtcgaacc cggtaaaacg atggcgcagc tgttccgcga ccacggactg
      541 cccgctaccg atgtctatgc aatggcgcag gtagaaggcg cgggtaagcc gctgagtaat
      601 ttgcaaaacg ggcagatggt gaaaattcgc cagaacgcca gcggcgtggt gacgggctta
      661 accattgata cggggaataa tcaacaggtc ctgttcaccc gccagccgga tggcagtttt
      721 attcgtgcgc ggtagatgaa gggtcaacca aaagccagct aaggattgtc tctttgatga
      781 ccttacgtct ggcttccagt gcctgggggg aaaccatact gatatgataa accaggccaa
      841 gcgtatgctg gttgatcaaa taatatccgc caagcgcagc aatattaata ttcacctgca
      901 atgggtcgat atctggctta aaaatattgt gtttcttccc ttcatccaat aaggattcca
      961 ttaactgcaa gtgcgcatgg ttaatttcta agagacgctg tgattttgca taatgcacac
     1021 ctttactttg attttcacta tgcacaattt ttaaaaacca ggggttagca atataataat
     1081 tccatgtaaa atcaataatt ttctcaatag ctttttgtgg cggaagatct gagaaatcaa
     1141 gggccttttc tttttgtcga atatcgttcc aggtatattc cagtacttta ataaataaat
     1201 tttctttatt accaaagtga tgataaaccg tctgtttact acaccccgca gccttaacaa
     1261 tattatcgac cctggcccct tcataaccat attcggcaaa ttcattaacc gcgcataaca
     1321 gcagcttttc cttcaggctc tgtacgttgc tggtatcaag atacataacg cttccccacg
     1381 acctcttcgt ggtgactatc ataacctgat ttgatttcag cttcagaaaa aagggcaaaa
     1441 acttgtactt tcatcaggtt atcaaatgtc ctgtgatcgt catcgcatat caaccaacta
     1501 gatggttgac ctttttatcc tccagtcgca agctaatcat caccaaggaa acaaaattca
     1561 aaaaagagag ctattgtatg aaatccactc gtcctgttgc cgtgattacc ggagccgctc
     1621 gcggtattgg aaagggctgt gcgcaggagc ttgctcgtgg cgggtttaac ctgctgatta
     1681 acgatcgtcc cgatgccgac agcgtagaaa aactgcatat cacacaacaa gaatgtttgg
     1741 cggaaggcgt ggaagtgatt tgcttccctg ctgatgtcgg tgatctatct ctgcatgagg
     1801 agatgctcga cgcggcgcaa aaccagtggg ggcggctgga atgtctgctc aataacgcgg
     1861 ggatttcagt gaaaaaacgc ggtgacctcc tcgacctcga accagacagc ttcgatcaaa
     1921 acattgccat taacacccgc gcgccgttct tccttgctca ggcattcagt aagcgcttac
     1981 tggcacagcc gaaaccggaa gcggagctgc cgcaccgctc gattattttc gtcagttcca
     2041 tcaacgccat catgctggcg atgaatcgcg gcgaatacac cattgccaaa accgccgttt
     2101 ctgccgctgc tcgtctgttt gctgctcgcc tttgtaatga acaaattggc gtttatgaag
     2161 tgcgccctgg tctcatcaaa actgatatga ctattccggc aacggcttat tacgacgaac
     2221 tgattgccaa aggtctggtg ccgtggggac gttggggtta tccggctgat attgcttcca
     2281 ccgtccgcgc gatggcggaa ggtaaactga tttacacctg tggtcaggcg gtcgccatcg
     2341 acggcggctt aagtatgccg cgcttctgag gctgaatcat gagagatctg ttacaacgcc
     2401 cggatttatt cagtatcaat accgcgacac tgggctataa aacgccgcta cccgccatta
     2461 ttgatgcctg cgccgcacgc ggtattggcg caattgcccc ctggcgcagg gagttgcagg
     2521 gtgaagatct ccagcaaatc acccgccagc ttgccgccag taacatgagc gtttccggtt
     2581 tgtgccgtag cacttactac accgcgccga cactggcgga acgcaaactg gcgattgatg
     2641 ataaccgccg ggcgctggac gacgcagcgg tgcttaacgc cgcctgttat atgcaggtgg
     2701 ttggcggtct gccaacgggc actaaagatc tatatgaagc gcgagaacaa gtgaaacaag
     2761 ggattcgcca gttgctgcca cattcgaaag atgtcggcgt gccaattgcg ctggagccac
     2821 tgcacccgat gacagctgcc gatcgctctt gcctgtgcac gttacgtcag gcgctggact
     2881 ggtgtgatga actcgatccc gatggtgagt ttggcctcgg cgtggcggtg gatgtttatc
     2941 acgtctggtg ggatccggat ctcgccagtc agatcctgcg tgccggaaaa cgcatcctcg
     3001 cgtttcatgt ttccgactgg ttagtgccga ccactgatct ggtcaatgac cgtggtatgc
     3061 cgggagatgg cgtcatcaat attccgtcaa ttcgtcggct ggttgaaaac gccggattta
     3121 atggtgctat tgaactggaa atattctcgc cttactggtg gcagaaagat attaacagca
     3181 cgctggatat tagcgtcgat cgcatcgcgc attattgtta aggaatgatc atgagtgacc
     3241 aacccgttct gttcagcaga gtggcggctt cctgccgcct gacattgaac cgtgaagata
     3301 aatgtcatgc tattaacgaa gagatgatcg aatctctcga ccattattta aatgaaattg
     3361 aaaacgacac cacattacga ttagtcgaat taacggcaac cggcgataaa ttcttttgtg
     3421 cgggcggcga tattaaatcg tggtcggcct attcgccatt agatatgggc agaaaatgga
     3481 ttaaacgcgg taatgacgtt tttaatcgcc tgcgaaacat accccaatta accgttgcga
     3541 atctcaacgg tcataccatc ggcggtggta ttgaactggc cttgtgctgc gatattcgta
     3601 ttgcccgccc cggcgcgaaa ttctctaacc cggaagtgat gctcggcatg gtgcctggct
     3661 ggatgggcat tgaacgtgtg cttaatcagg tcggtccggt ggtcggtcgc cagatgctga
     3721 tgctgggtaa acgcctgacg gcgcaggaag ctcaggcggc gaatttaatt gatgaagtgg
     3781 tagaaaaaga acaggtggaa agctggatgg cgaaccagtt agcgcagctg gaaaaatgcg
     3841 gtccggtggc actggcgcat attaagcaac tgattctggc actggaaaat aaacatgccg
     3901 attacccgca ccagctactg gccggattaa tgtccgccac tcaggattgc cagcaggcga
     3961 cgcgcgcctt tgccgaaaaa agtagcgtga gcttccataa tcaataagga aattgtatgc
     4021 gtaagataac aaccgccgag gcactggcgg cgcaaattca ggacggtgcg actattgcta
     4081 ttagcggtaa cggcggcggt atggtagaag ccgaccatat tctggctgct attgaagcgc
     4141 gtttcctgca aaccggacac cctcgcgacc tgacgctaat ccactcgctg ggtattggcg
     4201 atcgcgactg caaaggcact aaccgttttg cgcacgcaga gatgctcaaa cgcattattg
     4261 ccgggcactt tacctggtcg cccaaaatgc aggcgctggt aaaaaataac acgattgaag
     4321 cctactgctt tcctggtggc gtgattcagg cactgctacg ggagatcggc tccggacgtc
     4381 cggggctttt tacccacgtt gggctgggat cgtttgttga tccacgcaat ggcggcggta
     4441 agtcgaatga atgcactacc gacgacctgg tagaactgat tgaaatcgac ggtaaaacca
     4501 aacttcgtta tcgccctttc aaagtggatt acgcgatttt gcgtggcact tatgccgatc
     4561 ctcgaggcaa cgtcagcctt gaagaagaag cgattgatat ggatagctat tccatggcgc
     4621 tggcagcaca caacagcggc ggcaaagtgt tcgtacaggt acgcgatgtg ctggaagctg
     4681 gcgcgattga accacgccga gtcaaattac cgggcattct agttgatggc atcgttgaac
     4741 accgcaaaca gccacaaacc tatcttggcg gttacgacct gaccatcagc ggccaacatc
     4801 gccgtctgag ttctaacgac gctattgaac tggttagtca tccggtacgt cgcctgattg
     4861 cccgtcgggc agcacgcgaa ctggtagctg gcgcttcaac caactttggc tttggtattc
     4921 ccggcggcgt tccaggagta gcgctgcgcg aaggcgttcc ttaccaaagt ttatggctga
     4981 gcgtcgagca aggcgtacat aacggcatga tgctggatga tgcgtttttc ggctgcgccc
     5041 gcaacgccga tgccattatt ccatcactcg atcaatttga gttctacagt ggcggaggga
     5101 tcgatatcac cttcctcggc atgggagaga tggatcagta cggtaacgtc aacgtctccc
     5161 atctcaatgg caatctgatt ggccccggcg gatttctcga aattgcgcaa aatgcccgta
     5221 aagtggtgtt ctgcggcacg ttcgacgcta aaggcagcaa gatcgatata acaccagatg
     5281 gcttgcatat cgcccagtca ggtcaaatcc ctaaactggt taccaaggtg gaaaaaatca
     5341 cttttagcgc cgcctacgca cagcaaagtg gtcaggaagt gttgtatatc actgaacgcg
     5401 cagtattcca gttaacggca gaaggcgttg aattaattga aatcgcgccg ggtgtggaga
     5461 ttgagcgcga cattctgccg tatatggctt tccgtccaat tatcaagcac ccacgcctga
     5521 tggaaagtag cctgtttacg ccgatggagg atgcatgagt cagcttgatg acaccatcct
     5581 tgatgcgctg acgcacgtta ctttcccaaa gggttttgca caggcagagc ccgcatgggt
     5641 tgtcacggta gacggtgtta attacccact ctggcaaaca gatgccctgg tagtcggcag
     5701 tggtgcagca agattacgtg cggcagttga acttaaacgc cgccagcaaa atgtgctgat
     5761 cgctaccgcc gggttatata tggggacgtc ggcatgttct ggttccgata aacagacact
     5821 atttaccgcc gctaccgcgg gcaacggcga caacttcacc aaactggcag aggcactggc
     5881 gagcggtggg gcgatggatc acgacaccgc ttatgtcgaa gcggtaggtt ctctgcacac
     5941 tctaggcggg ttgcaatatc ttggtctgga attaccggaa gatcgctatg gcgcgattct
     6001 tcgttatcaa accgaccatg acgaagccgg gcgtgcaacc tcgtgtgggc cgcgtacctc
     6061 aaggttaatg gtgaaagtgc tgttggaaga agtacagcgc ctcgccattc cagtgttgac
     6121 cagtgcaaca gtgattaaac tgctgcatca gcgtgacgaa aacggcgaag accgcgtggc
     6181 aggggcaatc ctcgcgaccg gtcatcgcgc ccataaccca tgggggctgg caattgtgac
     6241 tgcgcccaat gtggtactgg caacgggcgg accgggcgag ctttatcgcg acagtgttta
     6301 tccacataaa tgcttcggct cgctggggct ggcgctggag gaaggtctga cgctaaccaa
     6361 tctgaccgaa agccagtttg gtataggcac gccgcgtagc acgtttccgt ggaatttatc
     6421 cggcacctat gtacaggtga tcccgtatat ctattccgtg gatgctgagg gtaacgagta
     6481 taacttcctc gcgattacta tcgcaccacc caggagctgg cttcaaacat tttccgtaaa
     6541 ggctatcagt ggccgttcca cgccactcgg gtgatggatt ttggctccag cttgttagat
     6601 atggcagtag cgcaagagca gcaatcaggg cgtcaggtat ttatggattt caatcgcaat
     6661 cctgaagcgg tgccgggtga cctgccgttc tcattagatc gactggacga cgacgttcgc
     6721 gcgtatctgg aaaataacga cgctctggca ccatcgccca tcgaacggct gcaacgaatg
     6781 aatccgctgt ctatctcgct gtataagatg cacggttacg atctcaccac acagcctttg
     6841 cagtttgcca tgaataatca gcatatgaat ggcggcattg aagtggatat ctggggacaa
     6901 acctccctgc ccggctgttt cgccgtgggg gaagttgctg gcacacacgg cgtcactcgc
     6961 cctagtggtg cagcattgaa tgccgggcag gtttttgctg ttcgtctggc acgttttatt
     7021 ggttgcacgc aaaagcggtc tgttgagggt gatgtcgcgc agctggtagc ctcatctctg
     7081 aatatagtca gagaagttat cgctcaggca ctggcaaacg agaacggaat ggcgctggta
     7141 acggtgaaag acaaaatcca ggcgcgaatg tctgattacg ccggatttat ttgtcatgcc
     7201 gataaagttc gtcatgccac ccgtgacgcc ctgctgctaa gtgagtttgt acaacaacac
     7261 ggtttaacca tcaatcacgc aggcgaaatt gccgaactgt ttatgtggcg gcatatgacg
     7321 ctgacttctg ccgccgtatt aacgcaattg acgcattata ttgacgcagg tggtggcagt
     7381 cgtggagcac ggatggttat tgatccacaa ggcaaatgcc tcccacaaac tcgtcgcggc
     7441 gcaaaagaag aatggcgttt tcgctctgaa cgtgctgaag acaaaaatca cagattaacg
     7501 attcaatatt cgcaaggttc ttttattacc gaagtgaagt ctttacgtat gcaaccgtgt
     7561 attaaaggta tttactttga aaaaaactgg ccaga
//
LOCUS       DADLFA010000180         7534 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000180 DADLFA010000000
VERSION     DADLFA010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7534
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.182"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(214..1752)
                     /gene="lnt"
                     /locus_tag="JQH65_003430"
     CDS             complement(214..1752)
                     /gene="lnt"
                     /locus_tag="JQH65_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HAY6140737.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(1777..2655)
                     /gene="corC"
                     /locus_tag="JQH65_003431"
     CDS             complement(1777..2655)
                     /gene="corC"
                     /locus_tag="JQH65_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HAY6140738.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            complement(2745..3212)
                     /gene="ybeY"
                     /locus_tag="JQH65_003432"
     CDS             complement(2745..3212)
                     /gene="ybeY"
                     /locus_tag="JQH65_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAY6140739.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            complement(3209..4288)
                     /locus_tag="JQH65_003433"
     CDS             complement(3209..4288)
                     /locus_tag="JQH65_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAY6140740.1"
                     /translation="MFSFRRHTDKRNGLNIDTREITLEPADNARLLSLCGPFDDNIKQ
                     LERRLGIEINRRDNHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLTI
                     KEARVLEQSAESVPEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTG
                     KTYLAVAAAVDALERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFE
                     MLGFEKVEKLIERNVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNS
                     KAVITGDVTQIDLPRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYE
                     AWEEAEQKRKAALAAERKREEQEQK"
     gene            complement(4402..5826)
                     /gene="miaB"
                     /locus_tag="JQH65_003434"
     CDS             complement(4402..5826)
                     /gene="miaB"
                     /locus_tag="JQH65_003434"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HAY6140741.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGSTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            5972..7147
                     /gene="ubiF"
                     /locus_tag="JQH65_003435"
     CDS             5972..7147
                     /gene="ubiF"
                     /locus_tag="JQH65_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HAY6140742.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            complement(7301..7375)
                     /locus_tag="JQH65_003436"
     tRNA            complement(7301..7375)
                     /locus_tag="JQH65_003436"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7341..7343),aa:Gln,seq:ctg)
     gene            complement(7413..7487)
                     /locus_tag="JQH65_003437"
     tRNA            complement(7413..7487)
                     /locus_tag="JQH65_003437"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7453..7455),aa:Gln,seq:ctg)
BASE COUNT         1726 a         1996 c         2043 g         1769 t
ORIGIN      
        1 ttcagcggat ggtagaaaaa tatttgtcat ggcgaagaac gggttgatcc aaatggacag
       61 gacgcgctcg cttgatccgc aggcaattat tcgtcatccc caatatggca atgtggcttg
      121 tatccctgaa ccgtaagaaa aaggggcatg ataaccatgc cccaaagctc aatgagaaag
      181 tacgttttgc tcatgcagga tacatcaggc acatcaacgt ttacgtgctc gcagactcat
      241 caacacagcg gcaaaaccaa acagtgccgt cagcacccac agtggccagt tgccggtacg
      301 tgcatacggt gtgagtccgg tggtcggtgt cacattagtg gttaacacct cgcgggtgaa
      361 ctgcgggatc atcgcctgaa tctcaccctg cgggccaatc accgccgtaa tgccgttgtt
      421 ggtgctgcgc aacagtgggc gcgccagctc cagcgcacgc attcgcgcca tctggaagtg
      481 ttgccatgga ccaatagatt taccaaacca cgcatcgttg gagatagtca gcagatagtc
      541 ggtatccggg cggaagttat cgcgcacttg ctcgccgaga atgatctcgt agcaaatagc
      601 cgcagtaagc tcaataccat ttactgacag cggcggctgg atatatggcc cacggctgaa
      661 cgacgacatc ggcagatcaa agaacggtgc taacggacgc agaatcgact ccagcgggac
      721 aaactcgcca aacggcacca gatggttttt gttatagcga tcggctgatt cgtagctgta
      781 cggtgcacct ttacccagtg tgatgatggt gttgtaggta tcgtagcggt tctgcttatt
      841 gagacgcgcg tcgacaatcc cggttaccag cgagctacct ttatcgcgca actcaccgtc
      901 cagtgctttg aggaacggtt gctgattaat ttccagatcg gttatcgccg actccggcca
      961 gataatcaac gatgatttgc ccatcagcgg agccgttgcg ttgtagtaaa tcttcagcgt
     1021 attaagaagc tgatctccat cccatttcag tgattgcgga atatcgccct gaaccatcga
     1081 aacctgaatg gttttctccg gttgcggggt aaaccattgg atgtaacgca gcgggaaggg
     1141 tagggcaaac agcacgacgg ccaccaccag cggacgccag ttgcgtttga ccaacgccag
     1201 tgccagcagg ccactaacca tcatcagcag gaagttaatg gcttccacgc ccattatcgg
     1261 tgccagccct tttaacgggc catcaatctg gctatagccg aactgtaacc acgggaagcc
     1321 tgtcagtacc caaccgcgca gaaactcggt cacttgccag agagcagggg cggcaatcgc
     1381 cacgcgcagc caggtggttt tcggccacag acgcgacagc acgccagcaa acagtccggt
     1441 atacagcgac aaatacgccg ccagcagcac caccaggaag atgttaaccg ggccaggcat
     1501 tccgccaaag gtcgcgatgc tgacatagac ccagttaata ccgctgccaa agaggccaaa
     1561 tccccagcaa aagccaatag cggcagactg gagtggacgg cggttaaagg tcaacgcctg
     1621 aagccccatc aacgaaataa tcgccgcagg ccagacgtcg taaggagaga aggccagcgt
     1681 tccgcaggca ccgaataata acgccagcag caggcgaatg cgctggcgtt caattaatga
     1741 ggcaaaagcc atgtagttat ctatccagtt tcggttttat tcatccagct tcggctgggg
     1801 tgagtcatcc gggattttga catgaacctg aataatacgt cgactgtcgg ccatcgccac
     1861 tttgaactgg taaccgtcga tgtcgatagt ttcgccacgc gccggaagat gcccaaacgc
     1921 ctgcatcacc agaccaccga tagtgtcgac ttcttcatcg ctaaagtggg tgccgaacgc
     1981 ttcgttgaag tcttcaatgg aagccagtgc gcgcacggtc caggtatgac gactcagctg
     2041 acggaagtcg atatcatctt cttcgtcata ttcgtcttca atctcaccaa caatcagttc
     2101 caggatgtct tcaatggtca ccagaccgga aaccccaccg aattcgtcaa tgacgatcgc
     2161 catgtggtaa cgctgagagc gaaactcttt cagcatccgg tctacgcgct tactttcagg
     2221 aacgacaacc gcctgacgta acactttgtc catgctgaag gcttcagcat cgctgcgcat
     2281 aaacggcagc aagtctttcg ccatcagaat cccttcaatg tgatctttgt cttcgctaat
     2341 caccgggaaa cgtgagtggg cggactcgat gatgacatca aggcattcgt ccagcgtctg
     2401 gttgcgtttc agggtaatca tctgggagcg ggggatcatg atgtcgcgaa cgcgttggtc
     2461 tgcgatgtcc atcacccctt cgagcatatc gcgcgtatct tcgtcgataa ggtcgttctg
     2521 cccggaatca cggatcagcg ccagcagttc gtcacggttt ttcggttcac cgtggaaaag
     2581 ttggctgagt aacagggaga aaaatccctt cttgttgctt atcgtgtcac tactgtgtga
     2641 attgtcgtcg ctcatggcgt cgtatgggtt ctcatgttag ttaatcaaaa cgccgtcgtt
     2701 aatcaccaac ggcggggacg tctgccagtc aaatgcctgg caaattattc tttctcggca
     2761 atgtacggat cctcatagcc cagagcaagc ataatctctg tttcgagggc ttccatttct
     2821 tctgcttcgt catcttcgat gtgatcgtaa cctaacaaat gcagactgcc gtgcaccacc
     2881 atatgcgccc agtgcgcctc cagtggtttg ccttgctcct gagcttcctt ctcaaccacc
     2941 tgacggcaga taaccagatc gcccagtagc gacatttcca tgccaggcgg cacttcaaac
     3001 gggaaggaga gcacgttggt cggcttatcc ttaccgcgat aggtcagatt cagactgtgg
     3061 ctttcggcgg tatcgaccac gcgaatagtc acttccgatt cttcctgaaa ctgcgggatc
     3121 accgcattca gccatgtttg aaactggctc tcttccggta acccggaatt atcttcacat
     3181 gccagttgta aatcgaggat cacctgactc atttttgttc ctgttcttcg cgcttgcgtt
     3241 ctgccgccag cgccgctttt cgtttttgtt cggcttcttc ccaggcttca taggcgttaa
     3301 cgatacgcgc caccacaggg tgacgaacca cgtcttcgct gtggaagaag ttaaagctga
     3361 tctcttcgac atcggccagt acttcgatgg cgtgacgtaa gcctgattta gtattacgcg
     3421 gcaggtcgat ctgtgtgacg tcgccggtga taaccgcttt tgagttaaaa ccgatacggg
     3481 tcaggaacat cttcatctgt tcgatggtgg tgttctggct ctcatcgaga atgataaacg
     3541 cgtcgttcag cgtacgacca cgcatatagg ccagcggcgc gacttcaata acgttgcgct
     3601 caatcagttt ctcaactttc tcaaagccca gcatttcaaa cagcgcgtcg tacagtgggc
     3661 gcagatacgg gtctactttc tggcttaaat cgccaggcag gaagcccagt ttctcaccgg
     3721 cttcgaccgc cggacgagtc agcagaatac ggcgaatctc ctgacgctcc agggcatcaa
     3781 ctgccgcagc cactgccagg taggttttac ccgtacccgc cgggccaacg ccgaaggtaa
     3841 tgtcatggtc gagaatattg gcgatgtact gcgcctggtt tggcgtgcgc ggcttaatta
     3901 cgccgcgttt ggttttgata ttgaccgctt tgccgtactc cggcacgctc tccgcacttt
     3961 gctccagtac tcgcgcttct ttaatcgtaa ggtggatctg ttccggttcg atatcctgaa
     4021 tctgaccgcg catcggggct gtatcgacat acaggctacg cagaatgtct gccgcagcgg
     4081 tgacgcaaat cggacggccg gtcagtttaa agtggttgtc gcggcgattg atctcgatgc
     4141 cgagacggcg ttcgagctgc ttgatgttgt catcaaacgg gccgcacagg ctcaacagac
     4201 gcgcgttgtc tgctggctcc agggtgattt cgcgagtgtc tatgttcaaa ccgttcctct
     4261 tatctgtatg ccgccggaag ctgaacattc accggcctat aaggaaatta ttcacgccac
     4321 aggaaaaagg cgcaagcatt gcgataaaga tggggataaa gagagaaaaa acaaggccca
     4381 ccggaacggc gggcctgaga attacggctg ataataaccc acgccaaggt cgttttcttt
     4441 gcgggtacgg gcaatcaccg attccggtgt ttctgccacg cgcaggccca tttcatcttc
     4501 agtacgcacc actttaccgc gcagagagtt cgggtagacg tcggtaattt ctacatcgac
     4561 gaatttaccg atcatatccg gtgtgccctc gaagttgacc acgcggttat tttcggtacg
     4621 cccggaaagc tccatgatgc tcttacgcga agtcccttct accagaatac gctgggtggt
     4681 gccgagcatc cggcggctcc atgccatcgc ttgctgattg atacgttctt gcagaatata
     4741 cagacgctgc ttcttctctt cttccggaac atcatcaacc atatcggcgg ctggtgtacc
     4801 cggacgtgca gagaagataa agctgtagct catgtcgaaa ttgacgtcgg caatcagctt
     4861 catcgttttc tcgaagtctt cggtggtttc gccagggaag ccaacgatga aatcagaact
     4921 gatctgaata tctggacgcg ccgcacgcag tttacggatg atcgctttgt actccagtgc
     4981 cgtatgagta cggcccatca ggttcagaat gcgatcggaa ccgctctgta ccggcagatg
     5041 caggaagctc accagctccg gcgtgtcgcg atacacttcg atgatatcgt cggtgaattc
     5101 gatcggatgg ctggtggtaa agcgaatacg atcgatcccg tcgatcgcag caaccagacg
     5161 cagcagatcg gcaaacgatc cggtggagcc gtcgtagttt tcaccacgcc aggcgttcac
     5221 gttctgaccg agcaggttga cttcacgcac gccctgagcc gcaagctggg caatctcaaa
     5281 cagaatatcg tcagacggac ggcttacctc ttcaccacgg gtgtaaggca ccacgcagta
     5341 ggtgcaatat ttattgcagc cttccatgat ggagacaaac gcggtcggcc cttcggcgcg
     5401 cggttccggt agacggtcaa acttctcgat ttccgggaag ctgatatcta caaccgggct
     5461 gcggtcgccg cgcacggagt tgatcatctc cggcagacgg tgcagcgttt gcggcccaaa
     5521 aataatatcg acatagtggg cgcgctggcg aatgtgctcg ccttcttgcg atgccacgca
     5581 gccaccgacg ccgataatca ggtctggatt cttctctttt aacagtttcc agcgacccaa
     5641 ctgatggaag actttttcct gagccttctc gcggattgag caggtgttca gcagcagcac
     5701 atccgcttct tccgccacgt cggtcagttg atagccgtgg gtggcatcca gcagatcggc
     5761 catcttcgat gaatcgtact cgttcatctg acagccccag gttttaatat ggagtttttt
     5821 ggtcatcgac ttgctcttgc gaaatagtag ccaggaatgc agggcgcata gtgtaatgct
     5881 ttgctgccgt tgtggccagt atgagcgtta tcagcccttg ggggtaaaaa tcctgtaaac
     5941 ttaaagcata ttgctaacag gatgattgac catgacaaat caaccaacgg aaattgccat
     6001 tgtcggcgga ggaatggtcg gcggcgcgct ggcgctgggg ctggcacagc acggattttc
     6061 ggtaacggtg atcgagcacg cagaacctgc accgtttgtc gctgatagcc aaccggacgt
     6121 gcggatctcg gcgatcagcg ccgcatcggt atcattgctt aaagggttag gggtctggga
     6181 tgcagtacag gctatgcgtt gccatcctta ccgcagactg gaaacgtggg agtgggaaac
     6241 ggcgcatgtg gtgtttgacg ccgctgaact taagctaccg ttgcttggct atatggtgga
     6301 aaacaccgta ctgcaacagg cgctgtggca ggcgctggaa gcgcatccga aagtaacgtt
     6361 acgtgtgcca ggctcgctga ttgcattaca tcgagataat gatcttcagg agctggaact
     6421 gaagggcggt gaaacgatcc gcgcgaaact ggtgattggt gccgacggcg caaattctca
     6481 ggtgcggcag atggcgggaa ttggcgttca tgcctggcag tatgcgcagt cgtgcatgtt
     6541 gattagcgta cagtgtgaga acgatcccgg cgacagcacc tggcagcaat ttaccccgga
     6601 cggaccgcgt gcgtttctgc cgttgtttga taactgggca tcgctggtgt ggtatgactc
     6661 tccggcgcgt attcgccagt tgcagaatat gaatatggcg caactccagg cggaaatcgc
     6721 gaagcatttc ccgtcgcgtc tgggttacgt tacaccgctt gccgctggtg cgtttccgct
     6781 gacgcgccga catgcgttgc agtatgtgca gccggggctt gcgctggtgg gcgatgccgc
     6841 gcataccatc catccgctgg cggggcaggg ggtgaatctt ggttatcgtg atgtcgatgc
     6901 cctgattgat gttctggtga atgcccgcag ttacggtgaa gcgtgggcca gttatcctgt
     6961 cctcaagcgt taccagatgc ggcgcatggc ggataacttc attatgcaaa gtggtatgga
     7021 tctgttttat gccggattca gtaataatct gccgccgctg cgttttgtgc gtaatctcgg
     7081 gttaatggcg gcagagcgtg ctggcgtgtt gaaacgtcag gcgctgaaat atgcgttagg
     7141 gttgtagcct tacaacattg ccggatgcgt gccaaccgta ggtcggataa gacgcggcag
     7201 cgtcgcatcc gacattgaag gataagacgt gtcaacatcg cattcgacat tgaatgaacg
     7261 cagaaaagca aaaagctcgc cgaagcgagc ttttttaatg tggctggggt acgaggattc
     7321 gaacctcgga atgccggaat cagaatccgg tgccttaccg cttggcgata ccccaactgg
     7381 gtgcacttac gatgtaagcg tcttgaataa attggctggg gtacgagggt tcgaacctcg
     7441 gaatgccgga atcagaatcc ggtgccttac cgcttggcga taccccaaca aattggtttt
     7501 tgaatttgcc gaacgtattc gacacattca gaat
//
LOCUS       DADLFA010000181         7473 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000181 DADLFA010000000
VERSION     DADLFA010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7473
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.183"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..339
                     /locus_tag="JQH65_003438"
     CDS             <1..339
                     /locus_tag="JQH65_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAY6140743.1"
                     /translation="QTEAFLNAYGLSRFAPLGYDPRDLPIRDLAGYRKKGNHDGDPII
                     FYTFPAAFEQEIAKGFNTKQFAEVLKNAGMLTPPTSGRGYQGRVREDGRQIRVYVLNF
                     MAEESSQPEE"
     gene            714..1148
                     /locus_tag="JQH65_003439"
     CDS             714..1148
                     /locus_tag="JQH65_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAY6140744.1"
                     /translation="MTAQISAYGRLVADPQTRTTTNGNNMAMARLAVSLPCNAAEAGE
                     STFWLGVIAFGKQADALAKHQKGDLVSVAGNMQLNQWTGQDGGTQQGYQVVADSVISA
                     RTVRPGGKAGQRGQATDALRRAQQPSRDEYDQRPPFDDETPF"
     gene            1159..1800
                     /locus_tag="JQH65_003440"
     CDS             1159..1800
                     /locus_tag="JQH65_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6140745.1"
                     /translation="MSTQPIIIEFGSYRWPRHLAIHEADHAIAAWFFGIQEVEIALSG
                     NNRVAKTYLSGDVDECGAVAVWHANYPRNVAEVKSYTLSNDDTRLRLIDQVCRAMVIS
                     LAGIVAQSRYTGDDINALMETSGKADMKNVRDLSEVYRVAGGEDADIQRISLSRAEAL
                     IALKWWDVIALAKILENRNHMPASNFQRIMGDIDKPVPKPMTLAELDELVGEE"
     gene            1797..2147
                     /locus_tag="JQH65_003441"
     CDS             1797..2147
                     /locus_tag="JQH65_003441"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6140746.1"
                     /translation="MKPYILAAGLISSAPALSAIHCGGFTIQPDRYRTTINGDVVHIV
                     DAQFYGAPRDFSQAAIKLLPDAITTTDRQYRLETQGGNALLELMTRENPPRVLNRERC
                     DAGLTSFGWYTSAP"
     gene            2841..3452
                     /locus_tag="JQH65_003442"
     CDS             2841..3452
                     /locus_tag="JQH65_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6140747.1"
                     /translation="MRDRTRSYTTDLPRIGLPFLANMRSRLADAEPGTQLYTQTESGT
                     LYAFRQADSYAMTINGVTRAIRTTTTKAGYGVREWYACPHCMKRAAKLYIGKKDIGCR
                     ACWKLHYKSQSADILDRMRMKIRQQRCAIWGSNDLANNLFNDIRMFPKPKGMRWATFD
                     RKRAELSVMEMAYWQAFSPVVDRITGVIERKAGKAARGIGLTL"
     gene            3684..3869
                     /locus_tag="JQH65_003443"
     CDS             3684..3869
                     /locus_tag="JQH65_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6140748.1"
                     /translation="MTPREIALLTIAKLEHGGHQLTQADQREIERSVNADIARRDRFR
                     EMMRAPAYQWKKPAPRR"
     gene            3990..4532
                     /locus_tag="JQH65_003444"
     CDS             3990..4532
                     /locus_tag="JQH65_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HAY6140749.1"
                     /translation="MYQLKITIRDSKPPIWRRVLVPEQIPFSELHAVIQLAFGWNDEH
                     LYMFEKGRKGDPGSEYRVWGEDESMGNAAITPLWAALQNDGDKLVYTYDFGDWWDCVI
                     VLEKQTHDTSNQPISCLRGKGTTPVENSGGLHGYNELLLQARESDNPEQAEIHNFLML
                     DIERRVYDLSSINDRLQTLN"
     gene            complement(4525..4884)
                     /locus_tag="JQH65_003445"
     CDS             complement(4525..4884)
                     /locus_tag="JQH65_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004202368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6140750.1"
                     /translation="MRMTSRKKEILSYFEPDNLEWVTGEIGAPPFDVSGVAYLLHGMV
                     SFDKRHQLESTRRTLESMVAGGLLERVTVYESRQIRRGGETNATVVRYGLPGQCAVMR
                     DTGGADNAISGEYMRVS"
     gene            5318..6142
                     /gene="dinD"
                     /locus_tag="JQH65_003446"
     CDS             5318..6142
                     /gene="dinD"
                     /locus_tag="JQH65_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HAY6140751.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKNQNILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGRKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            6434..7051
                     /locus_tag="JQH65_003447"
     CDS             6434..7051
                     /locus_tag="JQH65_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HAY6140752.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(7048..7473)
                     /gene="ligB"
                     /locus_tag="JQH65_003448"
                     /gene_synonym="yicF"
                     /pseudo
     CDS             complement(7048..7473)
                     /gene="ligB"
                     /locus_tag="JQH65_003448"
                     /gene_synonym="yicF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312549.2"
                     /note="this ligase is similar to LigA but it lacks the
                     C-terminal BRCT domain; catalyzes strand joining of nicked
                     DNA in the presence of a divalent cation and NAD+;
                     internal stop; incomplete; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
BASE COUNT         1998 a         1836 c         1909 g         1730 t
ORIGIN      
        1 cagacggagg cgtttttgaa tgcctatggg ctgagtcgat tcgcgccgct gggttatgac
       61 ccgcgcgatt tacccatccg tgatttagcc gggtacagga agaaggggaa ccacgacggc
      121 gacccgataa ttttctacac cttcccggca gcgtttgagc aggagattgc aaagggcttc
      181 aataccaaac agtttgccga agttctaaag aatgcaggaa tgcttacacc accaacgagc
      241 gggcgaggct atcaggggcg agtaagggag gatgggcgac agattcgcgt ctatgtactc
      301 aatttcatgg cagaggaaag cagccagcca gaagaataat tatttcttca catgcgtagt
      361 tttagtgttg tctcggttgt ctttgttgtc tcaatttgta attgagattg atttataagg
      421 attttatttt aatttctgag acaacaccga gacaacattt agccattttg agacaacagg
      481 gcgaaagtgt gtttttagtc tggcaggcag tgagacaaca tttatgccgg gtgagacaac
      541 acgaaattgc aaatgttgtc tcagcaaaac ccagacgtgg cgcggcctgt agagccgtga
      601 gacaacaaag acaacagaga caacactatt tttgcttact atgaaaaaaa atcggcaggt
      661 gtcccacatt aagaaatcgt cattagcaaa actaataatc cagtgagggg aaaatgacag
      721 cacaaatttc agcatacggg cggctggtgg cagatcccca gacccgcacc acaacgaacg
      781 gtaacaacat ggctatggcg cggctggcgg tgtctctccc ctgtaatgcg gcagaggcgg
      841 gagagtcaac cttctggctg ggcgttattg cttttgggaa gcaggccgat gcgctggcaa
      901 agcaccagaa aggcgacctt gtgagcgtgg caggcaatat gcaactcaac cagtggacag
      961 ggcaggacgg cggtacacag caggggtatc aggtagtagc cgacagtgtg atcagcgcca
     1021 gaacggtacg ccccggcggg aaggcaggac agcggggaca ggcaacggac gcgctgagac
     1081 gtgcgcagca gccatcccgt gatgaatacg accaacgccc accattcgac gatgaaacgc
     1141 cattctgaga gggaagatat gagtacgcaa cccatcatta ttgagttcgg cagctatcgc
     1201 tggcccagac atcttgcaat acatgaagca gatcatgcga tagccgcatg gtttttcggc
     1261 atacaggagg tagaaattgc gttatcgggt aataaccgag tggcaaaaac ctaccttagc
     1321 ggcgatgttg atgaatgtgg agctgtcgcg gtatggcacg ccaactatcc acggaatgtg
     1381 gctgaggtta agtcttacac cctctcgaat gatgacacca ggttacgtct gatagaccag
     1441 gtatgccggg ctatggttat ctctctggct ggtatcgtcg cgcaatcacg ctatacgggg
     1501 gatgatatta acgcactgat ggaaacgtcc gggaaagcag acatgaaaaa tgttcgtgat
     1561 ttatcagagg tataccgggt ggctggtggg gaggatgctg atatacagag aatatccctt
     1621 tcccgtgctg aggcgctgat tgccctgaaa tggtgggatg ttatcgcgct ggcgaaaata
     1681 ctggagaacc gtaaccatat gcctgccagc aacttccagc gcattatggg tgatattgac
     1741 aagcctgtac caaagcctat gacgctggct gaactggatg aattggtggg tgaagaatga
     1801 agccatacat tctggcagcc ggcctgatct cctcggcacc ggcactatct gctatccatt
     1861 gtgggggctt taccatacag ccagaccgtt atcggacaac gattaacggc gatgtggttc
     1921 atattgttga tgcgcaattt tacggcgcac cgcgagactt tagccaggcc gccataaagt
     1981 tattgccgga tgccattacc actaccgata ggcaataccg cctagagacg cagggaggca
     2041 atgccttgct tgagttgatg accagagaaa acccgccacg tgtgcttaat cgtgagcgct
     2101 gcgatgccgg gttaacaagt ttcggttggt acacttctgc gccgtgatgt tcgttccgag
     2161 cgcagcctaa aatattggac tgttcttgac ggatttaaaa ctctaatgga gccaagtcct
     2221 tgccagtagc gggctaggag cctaattgtg attcaggtcg tcaaaaatat tcaggtttgc
     2281 ggcattggtt cgcagtacgc agtccggtac gcggcaaaac tgcgcattta acgtgcgtac
     2341 cacctgacga attctaacgc cggcattgca aacagatgcg tgcgcaaaat ggctaagacg
     2401 tctgatattg ctggctttgg ctcaatcagt ttggaggtgg cggtatcagt acgatgctaa
     2461 tttttgctaa ggttttagta cgcaactcag gtacgcactc tcaccagata cgaaaccgat
     2521 tcgcagttgc gtaccagcct gtaaggcgca ttatcgctgg gtttctacag tcgaatgcag
     2581 tacgcaaagt ggaaaatcct ccgaatacga aacccgagca ttgcgcagta aattgcgaat
     2641 tgcgcagtta acaaaatatc tccgcaaaac gaaacggata tcaatgcgca aaccatcctc
     2701 aaaccccgcg cggttactgg ctttattgat gtgaaattgc aaaattggtg cgcaatttac
     2761 agtaaaatac gcatattatt tcgtattcaa tttgcgcacc gttcgcagtt aatgcgcagt
     2821 aattcgcagg aggccagcca gtgagagaca gaacgcgcag ctataccacc gacctgcccc
     2881 gtatagggtt gccattcctt gcgaacatgc gtagcaggct ggcggatgct gaaccgggta
     2941 cgcagcttta tacccagacc gaaagcggaa cgttgtatgc cttccggcaa gccgacagct
     3001 acgccatgac aatcaacggc gtgacgcggg ctattcgcac cacaaccacg aaggccgggt
     3061 atggcgtccg ggaatggtac gcttgccccc actgtatgaa gcgggctgca aagctgtata
     3121 tcggcaagaa agacattggc tgccgggcgt gctggaaact tcactataaa agccagagtg
     3181 ccgacatact ggacaggatg cgcatgaaga tacgccagca gcgatgcgcg atatggggca
     3241 gcaatgacct tgccaataat ctgtttaatg acattcgcat gttcccaaaa ccaaagggga
     3301 tgcgctgggc tacgtttgac aggaagcggg cagagttatc agttatggag atggcttact
     3361 ggcaggcatt cagcccggta gtggacagaa tcacgggcgt tattgagcgt aaagcaggaa
     3421 aagcagccag aggcattggc ctgactctat aaaagcaaaa cgcccgcacg gggcgtcaga
     3481 gaacgggata gacttctgcg acaatttgca ttcgccgcca atatagcaag ccgtccacat
     3541 caagcaataa actatcaccg ggagggggag gccaaatctc tgcaatcctg agccttaagg
     3601 actgcccgcc ccatcgtttt ttcatacccg cgaaaaatga aatttaatcc gggcgtgttt
     3661 catccttcca gagggtatga cgtatgacac cacgcgaaat agccttattg accatcgcca
     3721 aacttgagca tggaggccac cagcttacac aggcagatca acgggagata gaacgctcag
     3781 ttaatgccga tatagcccgg cgcgacaggt tccgcgaaat gatgcgagca cctgcctacc
     3841 agtggaagaa gccagcgccg cgcaggtaga tacgggatta ttccgcatgt aacttatgcg
     3901 tattaagcaa atttccttta ttcaccttta cagaattgtg agtacgtcag tatcgttatc
     3961 cttccgattc gcatttattc aggatttgca tgtaccagct aaagataacc atccgagaca
     4021 gcaaaccacc tatatggcgg cgtgtgctgg ttccagagca aatccccttt agtgaacttc
     4081 acgcggtgat ccagttggct tttggctgga atgacgaaca cctgtatatg ttcgagaaag
     4141 ggcgtaaagg tgatcccggt agcgagtatc gcgtatgggg tgaggatgaa agcatgggta
     4201 atgcggcaat cacaccacta tgggcagcgc tccagaatga tggtgacaag ctggtttaca
     4261 cgtatgactt tggcgactgg tgggattgcg tcattgtgct ggagaagcaa acacacgata
     4321 cgagcaatca gcccattagc tgcctgcgtg ggaaaggcac caccccggtg gaaaactcag
     4381 gcggcttgca tggttacaat gaattgctat tacaggccag agagtctgat aatcctgagc
     4441 aggccgagat ccacaatttc ctgatgctgg atattgagcg ccgggtttac gacctgagca
     4501 gcatcaacga cagattgcag actcttaact gacccgcata tattcgcctg atattgcgtt
     4561 atcagcgccg ccagtatcgc gcataacagc gcactgaccg ggcaaaccgt agcgcacgac
     4621 cgttgcgtta gtctcaccgc cgcgccgtat ctggcgcgat tcatacactg tcacccgctc
     4681 aagcagtcca ccagcaacca tgctttccag tgtgcgccgg gttgattcga gctggtgacg
     4741 cttatcgaac gacaccatgc catgaagcag gtaggccaca cccgacacat cgaacggcgg
     4801 cgcaccaatc tcaccagtca cccattcgag gttatccggt tcaaagtagc taagtatctc
     4861 ttttttgcgg ctggtcattc tcatagctgg caggttcctt attgtgggat agcactatca
     4921 tacaataagg cgcaaaagat aagaggccag aacagcaagc atgagagcat aaaacgagca
     4981 gccaaagaca gcgcctcaaa ataccgtgta cacaagggtg tacctactga gaaaacgcat
     5041 tacaagaaac acagcaacca cgggcgttta aagccactac tcaataaaca gattcaactc
     5101 gtaacgccaa ttcttacttt tcagccttca caaatgccgc ccactcaaac agagcggcat
     5161 ttttcttccc cgcaacttca attctgtttt gcgtgcctgc tccagatttt gcgatgtttt
     5221 tttgcccagc acactgagaa cgtgagatac tcacaactgt atataaatac agttacagat
     5281 ttactttctt tgcaatcgat atcacatgga gtgggcaatg aacgaacatc atcaaccttt
     5341 tgaagaaata cggcattatg gcactgaagg gcaagaattt tggtcagcta gagaattggc
     5401 accgttactg gattatcggg actggcgcaa ttttcaaaag gtactggccc gcgcgactca
     5461 agcgtgcgaa gccagcaatc aagctgcatc tgaccatttc gttgaaacca ccaaaatggt
     5521 tgtattaggt tcaggggctc aacgagaact tgaagatgtt catctctccc gctatgcctg
     5581 ttacctggta gtacaaaacg gcgaccctgc gaaaccggtt attgcggcag ggcaaactta
     5641 ttttgctatc cagacccgac ggcaggagct ggctgacgat gaagctttca ggcaacttcg
     5701 tgaagacgaa aagcgtcttt ttctgagaaa tgaattgaaa gaacataaca aacaattggt
     5761 tgaggcagcg cagcaagctg gggtagcgac agctatcgat ttcgccatat ttcagaatca
     5821 tggttaccgg gggctgtatg gtggattaga tcagaaagct attcatcagc ggaaggggct
     5881 gaaaaagaat cagaatatcc tggatcatat gggttcaaca gaactggcgg ctaatctctt
     5941 tcgagctacc caaacagaag aaaaactcaa gcgggatggc gtgaattcaa aacagcaagc
     6001 aaacaccaca cactttgacg tgggtcgcaa ggtgaggcaa accattcagg aacttggcgg
     6061 aaccatgcct gaggagttgc cgaccccgca ggtcagcatc aagcagttgg aaaacagtgt
     6121 aaaaattaca gagaagaaat agcagaaact aaaaactctt tttcttaatt gagatacccg
     6181 atgcgtaagc caaagtccag gtgcagtgaa cacaatggct aaacattgca cctttctttt
     6241 ccacccctca attttaacct attttttctt atgcattttc tcagacaaga actcagaata
     6301 atgcatctac gctacagaaa atagcgattt cacataacta caagttatca atttcctctt
     6361 accttaaaaa atctcaatcg tgacaatgcg cacaaatcgc taccctgcca gacagatttt
     6421 tagggagaga accatgctgt tacacatttt gtatttggtt ggcatcactg ccgaagccat
     6481 gacgggggcg ctggcggccg gacgacggcg catggataca tttggcgtaa ttattattgc
     6541 gaccgccacc gcaattggcg gagggtcagt gcgcgatatt ctgctgggcc actatccgct
     6601 cggctgggtc aaacacccgg aatatgtgat tatcgtcgct accgccgcag tactcaccac
     6661 tatcgttgct cctgtaatgc cttacctgcg caaagtgttt ctggtgctcg atgcgctcgg
     6721 actggtggtc ttttctatca tcggcgcaca ggttgcactg gatatggggc acggcccaat
     6781 tattgccgtt gtcgcggcgg taaccaccgg cgtgtttggc ggcgttttgc gcgatatgtt
     6841 ctgtaaacgc atcccactgg tattccagaa agagttgtat gctggtgtct catttgcctc
     6901 cgccgtgctg tacatcgcgc tgcaacacta tgtttctaac catgatgtgg tgattatctc
     6961 caccctggta ttcggctttt ttgcccgctt actggcgcta cgtcttaagc tgggattacc
     7021 ggtcttttac tacagccacg aaggtcacta aggttcaaaa cctgtgatct gctgggcagc
     7081 cagccaactg cccagcttct tgatttgcgc attttccttc cattcaataa cctgtctggc
     7141 gcgcccagat ccagtccccg gcagctgctg tcagaactgc tccgtgctaa ataaaagttg
     7201 cgaccaggac cgttcatcac tggcattaag cgccgcccgg gttagcggta ttcccattgc
     7261 catcacccag cgagtaaaag gctgcttacg agccagatta aactgatgcc ataactgcgc
     7321 acttttactt ttcgcgatcc ccggcgtgtt ctgtaattgc tctggcgtta ataaaagcca
     7381 agaaaagata tgctcaaagc gatgagtctg atgcagcgcg cgccaaccgg cctcaccaat
     7441 gccatccagc ccaagaacct gttttgaccc cag
//
LOCUS       DADLFA010000182         7435 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000182 DADLFA010000000
VERSION     DADLFA010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7435
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.184"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            167..274
                     /locus_tag="JQH65_003449"
     CDS             167..274
                     /locus_tag="JQH65_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6140753.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(327..1085)
                     /locus_tag="JQH65_003450"
     CDS             complement(327..1085)
                     /locus_tag="JQH65_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HAY6140754.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVTYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            1372..2301
                     /gene="pfkB"
                     /locus_tag="JQH65_003451"
     CDS             1372..2301
                     /gene="pfkB"
                     /locus_tag="JQH65_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HAY6140755.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISASQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            2402..2692
                     /gene="ghoS"
                     /locus_tag="JQH65_003452"
     CDS             2402..2692
                     /gene="ghoS"
                     /locus_tag="JQH65_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HAY6140756.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            2798..3658
                     /locus_tag="JQH65_003453"
     CDS             2798..3658
                     /locus_tag="JQH65_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAY6140757.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(3699..4235)
                     /locus_tag="JQH65_003454"
     CDS             complement(3699..4235)
                     /locus_tag="JQH65_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6140758.1"
                     /translation="MTYQQAGHIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            4382..5050
                     /gene="hxpB"
                     /locus_tag="JQH65_003455"
     CDS             4382..5050
                     /gene="hxpB"
                     /locus_tag="JQH65_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HAY6140759.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            5213..5803
                     /locus_tag="JQH65_003456"
     CDS             5213..5803
                     /locus_tag="JQH65_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY6140760.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQIINTLQIQFHRLIKHQVEY"
     gene            5936..7327
                     /locus_tag="JQH65_003457"
     CDS             5936..7327
                     /locus_tag="JQH65_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAY6140761.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
BASE COUNT         1866 a         1810 c         1837 g         1922 t
ORIGIN      
        1 attttcgata tatatgttga tgcataatat tattttttca taaaacgcag aaacagtgac
       61 caggtgtaca acgccaccca acggctgtaa taacaaaaaa tcccttgatg cccgtccctt
      121 ttgttacact ccgttatcac gcacaagaga tatgcaggac actggtatgc cgactaaacg
      181 ctttgataaa aaacactgga agatggtggt ggtgctactg gcaatctgtg gcgctatgtt
      241 gttgctacgt tgggcagcaa tgatttgggg ctgagcagtg acaaacgaag acagccagac
      301 cgcataacac ggtccggcag ataattttac atgctgtagc ccagcgataa ggttgtccga
      361 cggtcggtat gttccggtgc agactctgga ggttcagagt tccatgtaac gttgtaagtc
      421 accttcagtc caaaatgttc attaatagca acatttaatg cgctttcgga gttcagcgtt
      481 gtgtcttccg cgccaaagac cgaaacaccc tgcgtaaatt tagcgttgtc agtcaactgc
      541 catgcataag ccccggaggc atagcccagc ggctgagttt cactggcatt gtcggtatat
      601 ttgtcgtaac gcacacctgg accgaattca aagcggaaac tgtgtaccgg gccattcaaa
      661 aactgacgac cataacccgc ggtcaacaca tcgcgctctc gatagccgtt ataacggtct
      721 gtcagccagc ttgcctgacc aaataaatag tcataatcag ttaaattaaa gcggctacgc
      781 ccgcccgccg catatttttc tgaagaacgc tcatcattag aagaagtatt actggcgttc
      841 ccccacagcg accaggcagt ggtttgtcca taccaggtca tggtggtgtc agccgtaagt
      901 gaggagcttt tcgtattgcc tgattgtgca agatatcctg cgttcagatt accttcgaaa
      961 ggttttttag cgctggcagg atcgtccatg acagtaaaaa cggaatcatc ggcagctgca
     1021 ttcagtgacg caaacatgcc ccccgccaac ataacgatgg caggaactgt cttcaaaagc
     1081 ttcatttatc aagagtccgt acaacaaaaa aagagaccat cgcggtcccg gaaactttct
     1141 taaggatcaa agattagcgt ccctggaaag gtaacgaatt ataaaaaggc gcgaataact
     1201 tagcaatgta ttcttatttc attttttgaa taagcatgtg gcgaaaacag atttttattt
     1261 atatatattt atctgcaaaa ttttaaataa agctccaata aatcatattg ttaatttctt
     1321 cactttccgc tgattcggtg ccagactgaa atcagcctat aggaggaaat gatggtacgt
     1381 atctatacgt tgacacttgc gccctctctc gatagcgcaa caattacccc gcaaatttat
     1441 cccgaaggaa aactgcgctg taccgcaccg gtgttcgaac ccgggggcgg cggcatcaac
     1501 gtcgcccgcg ccattgccca tcttggaggc agtgccacag cgatcttccc ggcgggtggc
     1561 gcgaccggcg aacacctggt ttcactgttg gcggatgaaa atgtccccgt cgctactgta
     1621 gaagccaaag actggacccg gcagaattta catgtacatg tggaagcaag cggtgagcag
     1681 tatcgttttg ttatgccagg cgcggcatta aatgaagatg agtttcgcca gcttgaagag
     1741 caagttctgg aaattgaatc cggggccatc ctagtcataa gcggaagcct gccgccaggt
     1801 gtgaagctgg aaaaattaac ccaactgatt tccgcttcgc aaaaacaagg gatccgctgc
     1861 atcgtcgaca gttctggcga agcgttaagt gcagcactgg caattggtaa catcgagttg
     1921 gttaagccta accaaaaaga actcagtgcg ctggtgaatc gcgaactcac ccagccggac
     1981 gatgtccgca aagccgcgca ggaaatcgtt aatagcggca aggccaaacg ggttgtcgtt
     2041 tcccttggtc cacaaggagc gctgggtgtt gatagtgaaa actgcattca ggtggtgcca
     2101 ccaccggtga aaagccagag taccgttggc gctggtgaca gcatggtcgg cgcgatgaca
     2161 ctgaaactgg cagaaaatgc ctctcttgaa gagatggtcc gttttggcgt agctgcgggg
     2221 agtgcagcca cactcaatca gggaacacgt ctgtgctccc atgacgatac gcaaaaaatt
     2281 tacgcttacc tttcccgcta acaaaaacat tcccccagca ttgggggaat caccaccaac
     2341 ctgtcggcaa cgcgtttctc cgactatgct caaaagtcat gtgataacaa aggggtgaac
     2401 tatggccagt ggcgatcttg tccgttatgt cataaccgtc atgttgcatg aggatacatt
     2461 gactgaaatt aacgagttga ataattacct gactcgcgac ggttttttgc tcaccatgac
     2521 ggatgatgag ggaaatatcc atgagctggg gactaacact tttggactta tcagtaccca
     2581 aagtgaagaa gaaattagag aactggtttc ggggcttacc caaagtgcaa ccggcaaaga
     2641 tcctgaaatc accatcacga cctgggagga atggaatagc aacagaaaat aaatggtttt
     2701 tgggcaataa tcagtctgtg gtgtgcgtta gctcgtgttt ttacaccgca ttcttgcgct
     2761 aaccttatga tctggcagac aacatgggag agacatcatg tggcaggcaa tcagtcgtct
     2821 tttgagcgag cagttaggtg aaggcgaaat cgaactgcgt aatgaactgc ctggcggaga
     2881 agtccatgcc gcatggcatt tgcgctatgc aggacatgac tttttcgtca aatgtgatga
     2941 aagggaactg cttcccggtt ttaccgccga agccgaccaa ctggagttac tgtcgcgtag
     3001 taaaaccgtc accgtgccta aggtttgggc agtaggcgct gaccgtgact acagttttct
     3061 ggtgatggat tatctcccac ctcgtccgct ggatgcgcat agcgcattta ttcttggtca
     3121 gcaaattgcg cgtttacatc aatggagtga ccaaccacaa tttggcctcg attttgataa
     3181 ctcgctctcc acaactccac agcccaacac ctggcaacgt cgctggtcaa cgttttttgc
     3241 tgaacaacgg attggctggc agttggaact ggcagcagag aaagggatcg ctttcggcaa
     3301 tatcgacgcc atcgtcgagc atattcagca gcgtctggcc tcgcatcaac cgcagccttc
     3361 tctgttgcac ggcgatttat ggtccggcaa ctgtgcgctg ggtccggatg gcccgtacat
     3421 tttcgaccct gcctgctact ggggtgaccg agagtgcgac ctggcgatgt taccgctgca
     3481 tactgaacag ccgccacaaa tctatgacgg ctatcagtca gtatccccgc tacctgccga
     3541 tttccttgaa cgtcaaccgg tttaccaact ctacacgctg ttaaatcgtg caaggttatt
     3601 tggcggtcag catttggtta ttgctcagca gtcactggat agattattag cagcatgata
     3661 tgggttgaag ataatggccg ctctgtgcgg ccttttgatt aaataaaccc aagcagagaa
     3721 aagaacacat aacctgcagc aatacaaata accggcagga tatacagtga gaaaaactgc
     3781 aggaaaatag tatggtgcgg aacaacaata cgggactcaa tttgttcacg cgttagcccc
     3841 tcttcccctt tggctttttc cagaatgagt tgatcttcaa caccttcccg caagaagcgc
     3901 gcctggcgac tcattcgggc accggaatcc tgcaacgcca gtccgacaaa aatcaggatg
     3961 aaaatcaccc agaacataac gttcacgcca ccattaaaat tgggcgtcgg ggagttatac
     4021 cagaacagat tcaaaaaagg cgtattcgcc tgcatcatat cgatcatgac atgagtaaag
     4081 tcgagcatga ccgcattaat gccttcctgg ttttcctgcc gggtattcat aaatttcagc
     4141 aaagaaatca atgtagagat caatgcaggt ataaaaatca cccaacccaa aatccttttc
     4201 aaaacagcaa tgtgtccagc ttgttgatac gtcatgagtt ctccttgatt aagacgcgtc
     4261 gtttcactta gtttacctgt agatatctgt tttcgcccat tctttaaagg cgatatgata
     4321 ggcgcttaat cataagcacg gcttaatacc ttacacataa tgctctaaag gagaggttgt
     4381 aatgtcaacc ccgcgtcaga ttcttgctgc aatttttgat atggatggat tacttatcga
     4441 ctcagaacct ttatgggatc gagccgaact ggatgtgatg gcaagcctgg gggtggatat
     4501 ctcccgtcgt aacgagctgc cggacacctt aggtttacgc atcgatatgg tggtcgatct
     4561 ttggtacgcc cggcaaccgt ggaatgggcc aagccgtcag gaagtagtag aacgggttat
     4621 tgcccgtgcc atttcactgg ttgaagagac acgtccatta ttaccaggcg tgcgcgaagc
     4681 tgttgcgtta tgcaaagaac aaggtttatt ggtgggactg gcctccgcgt caccactaca
     4741 tatgctggaa aaagtgttga ccatgtttga cttacgcgac agtttcgatg ccctcgcctc
     4801 ggctgaaaaa ctgccttaca gcaagccgca tccgcaagta tatctcgact gcgcagcaaa
     4861 actgggcgtt gacccactga cctgcgtggc gctggaagac tcggtaaatg gcatgatcgc
     4921 ttctaaagca gcccgcatgc gttccatcgt cgttcctgcg ccagaagcgc aaaatgatcc
     4981 acgttttgta ttagcaaacg tcaaactctc atcgctgaca gaactcaccg caaaagacct
     5041 tctcggttaa tgaccagggg cagtgatcac gctgcccctg gttctttatc tgaatttgca
     5101 ttcaactgac ggattaatcg tcaatttaag agaaagagtt acaccgtcac cacttccgtg
     5161 cactgtataa aaatcctata ctgtacgtat cgacagttta gtgagtttta tcatgacggc
     5221 ggaaggtcac cttctctttt ctattgcttg tgcggtattt gccaaaaatg ccgagctgac
     5281 gcccgtgctg gcacagggtg actggtggca tattgtccct tccgcaatcc tgacgtgttt
     5341 gttaccggac atcgatcacc caaagtcgtt tcttgggcag cgattaaaat ggatatcaaa
     5401 accgatcgcc cgcgcttttg ggcatcgtgg ttttacccac agtctgctgg cggtatttgc
     5461 gctgctggca accttttacc ttaaggttcc ggaaggctgg ttcattccgg ctgatgcgct
     5521 acaaggaatg gtgctgggtt atttgagcca catacttgcc gatatgctga cacccgccgg
     5581 tgttcccctg ctctggccat gccgctggcg tttccgcttg cctatcctag ttccccaaaa
     5641 gggcaaccaa ctggaacgtt ttatctgtat ggcattattt gtctggtcgg tatggatgcc
     5701 ccattcatta cccgagaaca gcgctgttcg ttggtcatcg caaataatca ataccttgca
     5761 gatccagttt catcggctta ttaagcatca ggttgaatac taaaaagaca aaaatcacct
     5821 ttctggaata agcaattcca tttgaatata agagccagct cacagttctg ttaatcttgc
     5881 gccaacacta tgactgctac gcagtgatag aaataataag atcaggagaa cggggatgaa
     5941 ctttccatta attgcgaaca tcgtggtgtt cgttgtactg ctgtttgcgc tggctcagac
     6001 ccgccataaa cagtggagtc tggcgaaaaa agtgctggtg ggtctggtga tgggtgtggt
     6061 ttttggcctt gccctgcata ccatttatgg ttctgacagc caggtactta aagattctgt
     6121 acagtggttt aacatcgttg gtaacggcta tgttcaactg ctgcaaatga tcgttatgcc
     6181 gttagtcttc gcctctattc tgagcgcggt tgcccgtctg cataacgcat ctcagttagg
     6241 caaaatcagt tttctgacca tcggtacgct tttgtttacc acgctgattg cggcgctggt
     6301 cggtgtgctg gtcaccaacc tgtttggttt gacggctgaa ggtctggttc agggtggtgc
     6361 agaaactgca cgtctgaacg ccattgaaag taactatgtt ggtaaagtct ctgatctgag
     6421 cgttccgcag ctggtcttgt cctttatccc gaaaaacccg tttgccgatc ttaccggagc
     6481 caatccgacg tcaattatca gcgtggtaat ttttgccgca ttcctcggcg tagctgcatt
     6541 gaaactgctg aaggatgatg cgccgaaagg tgaacgcgtc ttagccgcta tcgataccct
     6601 acaaagctgg gtgatgaaac tggttcgcct tgtcatgcag ttgacccctt acggcgttct
     6661 ggctctaatg accaaagtgg ttgcaggttc taacttgcaa gacatcatca aactgggaag
     6721 tttcgttgtc gcgtcctacc tcggtctgct gattatgttt gcagtgcatg gcattctgct
     6781 gggcattaat ggcgtgagtc cgctgaagta cttccgtaag gtatggcctg tgctgacgtt
     6841 tgcctttacc agccgttcca gtgctgcgtc tatcccactg aatgtggaag cacaaacgcg
     6901 tcgtctgggc gttcctgaat ccatcgccag tttcgccgcc tctttcggtg caaccattgg
     6961 tcagaacggc tgcgccggtt tgtatccggc aatgctggcg gtgatggttg cgcctacggt
     7021 tggcattaac ccgctggacc cgatgtggat tgcgacgctg gtcggtattg ttaccgttag
     7081 ttccgcaggc gttgccggtg tcggtggtgg tgcaactttc gccgcactga ttgtgctgcc
     7141 tgcgatgggc ctgccagtaa ccctggtggc gctgttaatc tccgttgaac cgcttatcga
     7201 catgggccgt acggcgctaa acgttagtgg ctcgatgaca gctggcacgc tgaccagcca
     7261 gtggctgaag caaaccgata aagccattct ggatagcgaa gacgacgccg aactggcaca
     7321 ccgttaattc ttatgctggc aatggtccgt tttgtatagg ggccgttgct ttacttttta
     7381 aatgttccat cgcggcgatg catactgact catatttaaa cagatcgtca gcgaa
//
LOCUS       DADLFA010000183         7408 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000183 DADLFA010000000
VERSION     DADLFA010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7408
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.185"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            1..1458
                     /locus_tag="JQH65_003458"
                     /pseudo
     CDS             1..1458
                     /locus_tag="JQH65_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            1469..2542
                     /locus_tag="JQH65_003459"
     CDS             1469..2542
                     /locus_tag="JQH65_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY6140762.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMANVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVITHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKLGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            2790..3830
                     /gene="pstS"
                     /locus_tag="JQH65_003460"
     CDS             2790..3830
                     /gene="pstS"
                     /locus_tag="JQH65_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAY6140763.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            3917..4876
                     /gene="pstC"
                     /locus_tag="JQH65_003461"
     CDS             3917..4876
                     /gene="pstC"
                     /locus_tag="JQH65_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HAY6140764.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            4876..5766
                     /gene="pstA"
                     /locus_tag="JQH65_003462"
     CDS             4876..5766
                     /gene="pstA"
                     /locus_tag="JQH65_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAY6140765.1"
                     /translation="MAMVEIQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            5857..6630
                     /gene="pstB"
                     /locus_tag="JQH65_003463"
     CDS             5857..6630
                     /gene="pstB"
                     /locus_tag="JQH65_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HAY6140766.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            6645..7370
                     /gene="phoU"
                     /locus_tag="JQH65_003464"
     CDS             6645..7370
                     /gene="phoU"
                     /locus_tag="JQH65_003464"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAY6140767.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
BASE COUNT         1864 a         1717 c         1932 g         1895 t
ORIGIN      
        1 agatgttgat gtcagcgtgg gtgaattgcg cgatgaagac ggatttacac cggacgtcct
       61 tcaggcgcaa atacttcatg gtttttccca cgagatcact ttatatggag gtatgcaggc
      121 tgctgaaaat tatggttctg cagctctggg tgtcggtaaa gatcttggcg ctttgggcgc
      181 aatttctttc gatgtgacac atgctcgtgc gaattttagc catgatgata cagaaacggg
      241 tcagtcatat cgctttctct attcaaaacg atttgacgac acagacacta gcttgcgcct
      301 ggttggctat cgttactcca ccgagggcta ctataccctc aatgagtggg catcgcggcg
      361 caacagccct gaagactttt gggaaacagg taaccgacgt agtcgcgtgg agggaacgct
      421 aacgcagtcg ttggggagag attatggcaa tttatacctg acattaagcc ggcaacaata
      481 ctggcatacc gatgatgtcg aacgattaat gcaatttggc tacagcagta gctggaagcg
      541 tctctcgtgg aacgtctcct ggagttattc caatactgcc agacagggga cggggaacaa
      601 ccatgccagt gataacacca gtgagcagat ctacatgctc tctttatctg ttcctttatc
      661 gggctggtgg ggtaatagtt acgccaccta ttctgtttcg caaaacgata attccggtag
      721 ctcacatcaa gcggtacggc gctggaaaga aataaccttt catggaattt aatgcagtcc
      781 tataacagtc atgatgatga ggttggcggt aatatgtccc tgacctatga tggctcttat
      841 ggcacggtga acggcagcta taactacagc caaaattccc agaggctgaa ttatggtatc
      901 agagggggaa ttctggcaca cagcgaaggg gtaacgttaa gtcaggagtt aggtgaaact
      961 attgctcttg ttaaagcacc tggggccgcc gggttagaaa tagataatat gcgcggtgct
     1021 gcgacggact ggcgcggcta tacggtcaag acacagctaa acccttatga tgaaaatcgg
     1081 gtagcaatca gcgataacta tttctcgaag tcgaatatag aacttgataa taccgtcgtt
     1141 acgatggttc ccacgcgtgg tgcagtggtt aaagcggagt ttgtgactca tgtgggttat
     1201 cgcgttctct tcagagtgtt aaatgcaaat ggtaaaccgg taccttttgg agccattgct
     1261 gcgatacaag atgcaagttt ggcagattca ggaattgtcg gtgaccgtgg cgaactttat
     1321 ctttctggtc taccagaaaa aggacaggtt acgttatcct ggggagaaaa cgcctcaaca
     1381 aaatgcatct tcaattattc actttcgaca ccagaaagtg agagcggatt aattgaacag
     1441 ggtgtgacat gtcattaaaa ggatttttat gaacaagtat atcaaacagt ggtgctttgc
     1501 tgtgtttatg ctctcgttaa gtagtgtagc acttgcagct cctaaaggta tctgtacccc
     1561 tgataatggc gtatttcaca gtacgcttga tttctcggga tatcttatta ccgcgaacga
     1621 aaataaggtt ggaacgacat ttaacaccac tgtaactaat ggatcctcat atcctggacg
     1681 ctgtcattgt gatactggta acgtaggaga attcccctat atctattata cgtcaaaaat
     1741 aaaccaggca ttaacttatg ctggggttca ctctaatatt aattattatg atcttaatcc
     1801 gaaccttgat gttgggattg ctatagatat tcttggggtt gggtatgtga atgcaccttt
     1861 tgaatatcat gctaacaacc cgtctggtaa tacaaagtac aattgcaacc gcattgaacc
     1921 tttaagtata tctagtgggg ctaaagcgat agtatatttt tatatcaaga agacatttgc
     1981 aggaaaattg attattcctg aaacaaagat agtgacattg tatggaacaa ttagccgtga
     2041 caccccggtg gattactcac agccgatggc taatgtttat attcggggcg atattacagc
     2101 tccgcaaagt tgcgaaataa ataatttaca gccagtttat tttgatttta aagaaatacc
     2161 agctgcagat ttttcatctg ttgttggaag tgcggtaata acacataaaa ttactaaaac
     2221 ggtcactatt gagtgtgaaa acctggggat actgaatacc gatgatatca gtacgtcttt
     2281 ttatgctacc gaacccaata cggacaattc aatggtcgta acttcaaact ctaacgtggg
     2341 gataaaaatt tacgacaaaa ataataagga aatcaaagtg aacggtggtg agttgccaac
     2401 agacatgggt aaatcaacag tgtatggtga aaaattaggt agtgtaactt tttcagctgc
     2461 tcctgcgagc cttactggag ctcgcccagc cccggggcaa tttactgcaa cagcgacgat
     2521 aactgttgag attgtgcgct aataggtctg acaacgattg ttcatcacat cgttgtcatc
     2581 tcacccattt ctttatcact tttaaatgca tcatgacaaa aagatgacat gtgggatttt
     2641 catcttcaca tttaaaatca gtaagttatt tggtaattcc tttctgtcat aaaaatgtca
     2701 tattccttac atataactgt cacctgtttg tcctattttg cttctcgtag ccaacaaaca
     2761 atgctttatg aatcctccca ggagacatta tgaaagttat gcgtaccacc gtcgcaactg
     2821 ttgtcgccgc gaccttatcg atgagcgctt tctctgtgtt tgcagaagca agcctgacag
     2881 gtgcaggtgc aaccttccct gcgccggtgt atgccaaatg ggctgacact taccagaaag
     2941 aaaccggtaa taaagttaac taccagggta tcggttcttc cggtggcgta aaacagatta
     3001 tcgctaatac cgttgatttt ggtgcctctg acgcgccgct gtctgacgaa aaactggctc
     3061 aggaaggtct gttccagttc ccgaccgtga ttggcggcgt ggtgctggcg gttaacattc
     3121 cagggctgaa gtctggcgaa ctggtgctgg atggtaaaac cctcggcgac atctacctgg
     3181 gcaaaatcaa gaagtgggat gatgaagcca tcgccaaact gaatccgggt ctgaaactgc
     3241 cttcacaaaa cattgctgta gtacgccgcg cagatggctc cgggacttcc ttcgtcttca
     3301 ccagctacct ggcgaaagtg aacgaagagt ggaaaaacaa cgttggtact ggctctaccg
     3361 taaaatggcc gatcggtctg ggcggtaaag gtaacgacgg tatcgccgcg ttcgttcagc
     3421 gtctgccggg tgcaattggt tatgttgaat atgcttacgc gaagcagaac aacctggcgt
     3481 acaccaaact gatctccgct gatggtaaac cggttagtcc gaccgaagaa aacttcgcta
     3541 atgcagcaaa aggtgcagac tggagcaaaa ccttcgctca ggatctgacc aaccagaaag
     3601 gcgaagatgc atggcctatt acctctacca cgttcattct gatccacaaa gatcagaaga
     3661 aaccagaaca aggcacagaa gtgctaaaat tcttcgactg ggcgtacaaa accggggcta
     3721 aacaggcgaa cgacctggat tacgccagcc tgccggatag tgtagttgaa caggttcgcg
     3781 ctgcgtggaa gaccaatatt aaagacagta gcggtaagcc gctgtactaa taaaactcca
     3841 ggccgggtac ggtgttttac gccgcatccg gcattacaaa atgactttgt aaacgcgttt
     3901 aactgaagag taacttatgg ctgcaaccaa gcctgctttt aacccaccgg gtaaaaaggg
     3961 cgacataatt ttcagcgtgc tggtaaaact ggcggcgctg attgtgctat tgatgttggg
     4021 tggcattatt gtctctctga tcatctcctc ctggccgagc attcagaaat ttggtctggc
     4081 tttcctatgg accaaagagt gggatgcacc gaacgatatc tacggggcgc tggtgccgat
     4141 ctacggtacg ttggtgactt cgtttatcgc gctgctgatc gccgtcccgg tgagtttcgg
     4201 tatcgccctg ttcctgactg agcttgcgcc tggctggctg aaacgcccgc tgggtatcgc
     4261 cattgagctg ctggcagcca ttccaagtat cgtttacggc atgtggggcc tgtttatctt
     4321 tgcgccgctg ttcgccgttt actttcagga gccggtcggc aatatcatgt cgaatatccc
     4381 gattgttggc gcgctgttct ctggccccgc atttggtatc ggtatcctcg cggcaggcgt
     4441 gatcctcgcc atcatgatta ttccgtacat tgcggcggta atgcgtgatg tgttcgaaca
     4501 aaccccggtg atgatgaaag agtcggccta cggtattggc tgcaccacct gggaagttat
     4561 ctggcgtatc gttcttccgt tcaccaaaaa tggtgttatc ggcggcatca tgctggggct
     4621 gggccgcgcg ctcggtgaaa ccatggcggt gacctttatc atcggtaaca cctaccagct
     4681 cgacagcgcc tcgctgtaca tgccgggcaa tagtatcacc tctgcgttgg cgaacgaatt
     4741 tgcggaagcg gaatccggtc tgcacgttgc cgcactgatg gaactgggcc tgatcctgtt
     4801 tgtgattacc ttcatcgtcc tcgccgcatc gaagtttatg attatgcgcc tggctaagaa
     4861 tgagggggca cgctaatggc tatggttgaa atccaaacca ctgcggcgct ggctgaatct
     4921 cgccgcaaaa tgcaggcgcg tcgccgcctc aaaaaccgta ttgcgctgac gctctcgatg
     4981 gcgacgatgg ccttcggcct gttctggctg atctggattt taatgtccac catcactcgc
     5041 ggtatcgacg gtatgtcgct ggcgctgttc actgaaatga cgccgccgcc caatacggaa
     5101 ggtggtggtc tggcgaacgc cctggcgggt agcgggctgt taattttgtg ggccacggta
     5161 ttcggtacgc cgctgggcat tatggcgggg atttatctgg cggaatatgg tcgtaaatcc
     5221 tggctggcag aagtgattcg cttcattaac gacattctgc tctccgcgcc gtcgattgtg
     5281 gttggtctgt ttgtttacac catcgtggtg gcgcagatgg agcacttctc tggctgggcg
     5341 ggcgtgattg ccctggcgct gttgcaggtg ccgattgtta tccgcaccac cgagaacatg
     5401 ctgaaactgg tgccggacag cctgcgtgaa gcggcttatg cgctgggtac accgaagtgg
     5461 aagatgatct ctgcgattac gctgaaagcg tcggtgtccg ggatcatgac cggtatcctg
     5521 ctggcgattg cccgtattgc tggtgaaacc gcgccgctgc tgtttaccgc gctctccaac
     5581 cagttctgga gcacggacat gatgcagccg atcgccaacc tgccggtgac gatctttaag
     5641 tttgcgatga gcccgtttgc ggaatggcaa caactggcct gggccggggt attgattatt
     5701 accctgtgcg tactgctgct gaacattcta gcgcgcgttg tttttgcgaa gaataaacac
     5761 ggttaacact ttgccggatg cggcgtgaac gccttatccg gcctacggta agcctgatta
     5821 gcgaagtgca tcaggcacga tgaggaaaag attgcaatga gtatggttga aactgccccg
     5881 agtaaaattc aggttcgtaa tttgaacttc tactacggca aattccatgc cctgaaaaac
     5941 atcaacctgg atatcgctaa aaaccaggtt acggcgttta tcgggccgtc cggctgcggt
     6001 aaatcgacgc tgctgcgtac cttcaacaaa atgtttgaac tgtacccgga gcagcgtgcg
     6061 gaaggtgaaa ttctgcttga tggcgacaac atcctgacca actctcagga tatcgcactg
     6121 ctgcgtgcga aagtgggcat ggtgttccag aaaccgacgc cgtttccgat gtccatctac
     6181 gacaacatcg cttttggcgt tcgtctgttt gagaagctct cccgtgccga catggacgag
     6241 cgcgtgcagt gggcattgac caaagccgca ttgtggaacg aaaccaaaga taaattgcac
     6301 cagagcggtt actctctctc tggtggtcag caacagcgtc tgtgtattgc gcgtggtatc
     6361 gccattcgcc cggaagtgct gctgctcgac gaaccgtgtt cggcgctcga ccctatctct
     6421 accgggcgta ttgaagagct gatcaccgaa ctgaagcagg attacaccgt ggtgatcgtc
     6481 acccacaaca tgcagcaggc tgcgcgttgt tccgaccaca cggcgtttat gtacctgggc
     6541 gaattgattg agttcagcaa cacggacgat ctgttcacca agccagcgaa gaaacaaaca
     6601 gaagactaca tcaccggtcg ttacggttga ttcaggagtg cgttatggac agtctcaatc
     6661 ttaataaaca tatttccggc cagttcaacg ccgaactgga aagtatccgc acgcaggtga
     6721 tgaccatggg cggcatggtg gagcagcagc tttctgatgc aatcaccgcg atgcataacc
     6781 aggacagcga tctggcgaag cgcgtgatcg aaggcgacaa gaacgtcaac atgatggaag
     6841 tggcgatcga tgaagcctgc gtgcgcatta tcgccaaacg tcagccgacg gcgagcgacc
     6901 tgcgactggt tatggtgatc agtaaaacca ttgccgagct ggagcgtatt ggcgacgtgg
     6961 cggacaaaat ctgccgtact gcgctggaga aattctccca gcagcatcag ccgttgctgg
     7021 taagtctgga gtcgctgggc cgtcatacca tccagatgct gcacgacgtg ctggacgcgt
     7081 tcgcgcggat ggacattgac gaagcggtac gtatttatcg tgaagataaa aaagtcgatc
     7141 aggaatacga aggtattgtt cgtcaactga tgacctacat gatggaagat tcgcgtacca
     7201 ttccgagcgt acttactgcg ctgttctgcg cgcgttctat cgaacgtatt ggcgaccgct
     7261 gccagaatat ttgtgagttt atcttctact acgtgaaggg gcaggatttc cgtcatgtag
     7321 gcggtgatga gctggataaa ctgctggcgg gaaaagatag cgacaaataa ttcaccagac
     7381 aaatcccaat aacttaatta ttgggatt
//
LOCUS       DADLFA010000184         7206 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000184 DADLFA010000000
VERSION     DADLFA010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7206
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.186"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            305..394
                     /locus_tag="JQH65_003465"
                     /pseudo
     CDS             305..394
                     /locus_tag="JQH65_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
     gene            418..4932
                     /locus_tag="JQH65_003466"
                     /pseudo
     CDS             418..4932
                     /locus_tag="JQH65_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
     gene            4933..6582
                     /locus_tag="JQH65_003467"
     CDS             4933..6582
                     /locus_tag="JQH65_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2300 domain-containing protein"
                     /protein_id="HAY6140768.1"
                     /translation="MNWRRIVWLLALVTQPTLAEETPLQLALRGAQHDQLYQLSSSGV
                     TTVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIHTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            6587..>7206
                     /locus_tag="JQH65_003468"
     CDS             6587..>7206
                     /locus_tag="JQH65_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6140769.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKIQQGRLVVNGASMPQRVESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQV"
BASE COUNT         1726 a         1813 c         2020 g         1647 t
ORIGIN      
        1 cggttggtcg ccctaccttt tcatcaagcc agtagtctgc ccaccactgc atcatttctc
       61 tgcgcttatc gagatactga gcatggttgt aaattcaact aacccagtgc gttgacaaaa
      121 aattagcgca agaagacaaa aaatcacctt gcgctaatgc tctgtatcag gtcactaata
      181 ccagctaagt agttgattca tagtgactgc atatgttgtg ctttgtcgca ttttgtagtc
      241 tattatttaa tctgtaacaa cgataataca atgaatttta attggttttt gtgattttgt
      301 ccttcttctg acatggaagg acacccgatg gatacccaac gattccaatc ccaatttcat
      361 tggcatttat cgtttaaatt ttctggcgcg atagcagcgt gcttgtctct gtctcttgtg
      421 ggaacagggc ttgctaatgc tgatgattcg cttccttcca gtaactatgc gccgcccgcc
      481 gggggaacat tctttttgct tgctgacagc agttttagca gcagtgaaga ggcgaaagtg
      541 cgactggaag cgccggggcg tgattatcgg cgctatcaga tggaagagta cggcggcgtg
      601 gacgttcgcc tgtatcgtat tcctgacccg atggcatttt tgcgccagca gaaaaacctg
      661 catcgcattg tggtgcaacc gcaatatctg ggcgacgggc tgaacaatac gctgacctgg
      721 ctgtgggata actggtacgg caaatctcgc cgcgtaatgc agcgtacttt ctcttctcag
      781 tcacggcaga atgtgactca ggcattaccc gaattacatc tcggcaatgc cattattaaa
      841 ccttcccgtt atgtacagaa caaccagttt tccccgctga aaaaatatcc cttggtggaa
      901 cagttccgtt atccactatg gcaggctaaa ccggtcgagc cgcagcaagg ggtaaaactg
      961 gaaggcgcat ccagcaattt catctcgccg cagccgggta acatttatat tcctctcggc
     1021 caacaagagc cgggactgta cctcgtcgag gcgatggttg gtgggtatcg ggcgacgacg
     1081 gtggtgtttg tttccgatac cgtggctctt agcaaagtgt caggcaacga gcttctggtg
     1141 tggaccgcga gtaaaaaaca gggtgaagcg aagcccggct cagagatctt gtggactgac
     1201 ggtcttggcg tgatgacccg cggtgtgacc gatgacagcg gtaccttgca gttacaacat
     1261 atatcgccag aacgttcata cattctgggt aaggatgctg aaggcggcgt ttttgtctcc
     1321 gagaacttct tctacgaaag cgaaatctac aacacccgct tgtatatttt taccgatcgc
     1381 ccgctatatc gcgcaggcga tcgtgtcgat gttaaagtga tgggccgcga gttccacgat
     1441 ccgttgcatt catcccccat cgtcagcgcc ccggcgaagc tttcggtgct ggacgctaac
     1501 ggcagtctgt tgcaaaccgt cgatgtcacg ctggatgcgc gcaatggcgg gcagggaagt
     1561 ttccgcctgc cagaaaatgc cgttgccgga ggttatgagt tacgtcttgc ttaccgcaat
     1621 caggtctata gcagcagttt tcgcgtggca aactacatca agccacattt cgagattggt
     1681 ttagctctcg acaaaaaaga gttcaaaact ggcgaagcgg tcagcggcaa actgcaactg
     1741 ctctacccgg atggcgagcc ggtaaaaaat gcccgcgtgc agttaagttt gcgcgctcag
     1801 caattatcaa tggtcggtaa cgatttgcgt tatgccggac gtttccccgt gtcgctggaa
     1861 ggcagcgaaa cggtgtccga cgccagcggt catgtggcgt taaatctccc cgccgccgat
     1921 aaaccgagcc gttatttgtt aaccgtctcc gccagtgacg gcgcggcgta tcgtgtcacc
     1981 accaccaaag agatcctcat tgaacgcggt ctggcgcatt actcattaag tactgccgca
     2041 caatacagta atagcggcga gtcggttgtg ttccgttatg ccgcgctgga atcttcaaaa
     2101 caggttcctg ttacgtatga atggttgcgt ctcgaagacc gcacgagcca tagcggagag
     2161 ctaccgtcag gcggcaaatc ctttaccgtc aatttcgcta aacctggcaa ctacaatctg
     2221 acattacgcg ataaagacgg cttaattctc gctgggttaa gttatgccgt cagcggtaag
     2281 ggcagcacgg cgcatacggg tacggtagat atcgtggcgg ataaaacgct gtaccagcca
     2341 ggcgaaaccg cgaagatgct gattaccttt ccggagccaa ttgatgaagc attattgacg
     2401 ctggaacgcg atcgcgtgga acagcagtcg ctgctttcgc atccggcaaa ctggctaacg
     2461 ctacaacgtt taaacgatac ccagtatgaa gcccgggttc cagtgagcaa ttcctttgcg
     2521 cctaacatca ctttttcggt gctgtatacc cgtaatggtc agtacagttt tcagaacgcc
     2581 gggatcaaag ttgccgttcc tcagcttgat atccgggtga aaacggacaa aacccattac
     2641 cagccaggtg aactggtcaa tgtcgaatta acctcgtcgc tgaaaggtaa acctgtttct
     2701 gcgcagctaa cggtaggcgt ggtcgatgaa atgatctacg cgctgcaacc agaaatcgcg
     2761 ccgaatatcg gcaaattttt ctatccgctg gggcgtaaca atgtgcgtac cagctccagt
     2821 ctgtcgttta tcagctacga ccaggcgctc tccagcgagc cggttgcgcc tggcgcaact
     2881 aaccgcagcg agcggcgagt aaaaatgctt gaacgtccac ggcgtgaaga ggtggatacc
     2941 gcggcatgga tgccgtcact cacaaccgat aaacaaggca aagcgtattt cacgttcctg
     3001 atgcctgatt cgttaacccg ctggcgtatc accgcgcgtg ggatgaacgg cgacgggctg
     3061 gtcgggcagg ggcgtgctta tctgcgttcg gaaaaaaatc tctacatgaa gtggagtatg
     3121 ccaacggtgt atcgcgtggg cgacaaaccg gcggcaggac tgtttatctt cagtcagcag
     3181 gataacgaac cggtggcgct ggtgactaaa tttgcaggcg ctgagatgcg ccagacgctg
     3241 acgctgcaca aaggggcgaa ttatatttcg ctggcgcaga atattcagca atctggcttg
     3301 ttaagtgcag aactgcaaca aaatgggcaa gtgcaggaca gcattagcac aaaactgtct
     3361 tttgtggaca acagcaggcc cgttgaacag cagaaaaatg tcatgctcgg cggtggcgag
     3421 aacgcgctga tgttgcccga gcaggcgagt aatatccggc tacaaagtag tgaaacgccg
     3481 caggagattt tccgcaacaa tcttgatgcg ttagtcgatg aaccgtgggg tggggtgatc
     3541 aacaccggta gccgtctgat cccgctcagt ctcgcctggc gttcgcttgc cgatcatcaa
     3601 agtgccgccg ctaacgacat tcgtcagatg attcaggata accgtctgcg gctgatgcaa
     3661 ctggcggggc ccggagcgcg ctttacctgg tggggtgaag atggcaatgg tgacgccttc
     3721 cttacggcat gggcatggta cgccgactgg caggccagct aggcgctcgg cgtaacgcaa
     3781 caaccggaat actggcagca tatgctcgac agttatgccg agcaggcgga taacatgccg
     3841 ttattgcatc gggcgctggt gctggcgtgg gcacaggaga tgaatctgcc gtgcaaaacg
     3901 ttgttgaaag ggttggatga agctatcgcc cggcgcggaa ctaaagatga agatttctct
     3961 gaggaagaca tccgcgatat caatgacagc ctgatcctcg atacaccgga atctccactg
     4021 gcagatgcgg tggcaaacgt cttaaccatg acgttgctga aaaaagcgca gttgaagtcc
     4081 acggtgatgc cacaggttca gcaatatgcg tgggataaag cggcaaacag caatcagccg
     4141 ctggcgcaca cggttgtgct gctcaatagc ggtggtgacg ctacccagac ggccgctatt
     4201 ttaagtggtt tgaccgctga gcaatccact attgagcgcg cgctggccat gaactggctg
     4261 gcgaaatata tggcgacaat gcctccagtt gttttgcctg cgcctgcggg cgcatgggct
     4321 aaacataagt taactggagg gggcgaatac tggcgttggg ttggtcaggg cgtgccggac
     4381 attctctctt ttggtgacga attatcgccg caaaatgtgc aggtccgctg gcgtgagccg
     4441 gcaaaaatgg ctcaacaaag taacattccg gtgaccgttg aacgccagtt gtatcggctt
     4501 atccctggtg aagaagagat gagctttatt ctgcaaccgg tgaccagcaa tgagattgac
     4561 agcgatgcgc tgtatctcga tgacatcacg cttaccagcg agcaggatgc agttctgcgc
     4621 tacggtcagg tggaagtacc gctgccaccg ggagccgacg ttgagcgcac aacatggggc
     4681 atttcggtca ataaacccaa cgccgcgaaa cagcaggggc aattgctgga aaaagcgcga
     4741 aatgaaatgg gcgaactggc ctatatggtg ccggtgaaag aactgacggg aacggtcact
     4801 ttccgccatt tgctgcgctt ctcgcaaaaa gggcaattcg ttctgcctcc tgctcgttat
     4861 gtgcgttcct atgcacctgc acagcaaagt gttgcggcag ggagtgaatg gaccgggatg
     4921 caggtgaaat aagtgaactg gcgcagaatc gtctggctat tggcgttggt tactcagcca
     4981 acgcttgcgg aggagacgcc gttacaactg gcactgcgtg gtgcgcagca cgatcaactg
     5041 tatcagctgt cgtcatcagg cgtgaccaca gtctcagcat tacctgactc gctcaccacg
     5101 ccgttaggga gtttgtggaa actgtatgtt tacgcctggc tggaggatac ccatcaaccg
     5161 gagcagccct accagtgtcg cggaaactcc ccggaagagg tttattgttg tcaggctggg
     5221 gagagcataa cgcgcgatac cgcgctggta cgttcctgtg ggctctattt tgcgccgcag
     5281 cggttgcata ttggcgcaga tgtgtggggg caatactggc aacagcgtca ggcgcctgcg
     5341 tggttagcgt ctcttactac gctgaagcca gaaacctcgg taacggttaa atcgctgctt
     5401 gattcattag cgacgttacc tgcgcaaaac aaagctcagg aagtcttact ggatgtggtg
     5461 ctggacgagg cgaaaatcgg tgtcgcatcc atgctcggta gtcgggtacg ggtgaaaacc
     5521 tggagctggt ttgccgatga taaacaggaa atccgtcagg gtggttttgc cggctggtta
     5581 accgatggta caccgctgtg ggttactggc agtggcacca gcaaaacggt actaacccgt
     5641 tatgcgacag tgcttaatcg ggttttgcca gtgccgacgc aggttgccag tgggcaatgt
     5701 gtagaggtgg agctgtttgc ccgctatccg ctgaagaaaa tcacagcgga aaagagtacg
     5761 acggcagtaa aacccggcgt acttaatggg cgctatcgtg tgacgtttac taatggtaac
     5821 cacattacgt ttgtcagcca cggtgagacg accttactga gcgaaaaggg caaactgaaa
     5881 ttgcagtcgc atctcgatcg tgaagagtac gttgccagag ttctggatcg cgaggcgaaa
     5941 agtacgcccc ctgaggcagc gaaagccatg accgtggcga ttcacacttt tttacagcaa
     6001 aacgccaatc gggaaggcga ttgcctgacc atccccgaca gcagcgccac gcagcgtgtt
     6061 tctgcttcgc cagcgaccac gggggcgcga acgatgaccg cctggacgca ggatctcatc
     6121 tatgcaggcg atccggttca ttatcatggc agtcgagcta ccgaagggac actttcctgg
     6181 cggcaagcca cggcccaggc ggggcaggga gagcgttacg accagatact cgcgtttgct
     6241 tatcccgaca acagccttag ccgctgggga gcgccacgct caacctgcca gttattaccc
     6301 aaagcgaaag cctggctggc gaaaaaaatg ccgcagtggc ggcgtatatt acaaggcgag
     6361 acggggtaca acgaaccaga cgtgtttgcg gtctgtcgtt tagtctcagg tttcccctat
     6421 accgatcggc agcagaaacg gctgtttatt cgtaacttct ttacgcttca ggatcggctg
     6481 gatttaaccc atgagtatct gcacctggcc tttgatggtt atcccaccgg gcttgatgag
     6541 aactatatcg aaacgctgac ccgacaatta ttaatggact aatgctatgc gaaagatttt
     6601 tcttccgttg ttactggtgg cactttcgcc tgtcgctcac agtgaaggtg tgcaggaagt
     6661 cgagattgac gcaccgctct ctggctggca tccggcagag ggcgaagatg ccagtttttc
     6721 acaatcgatc aattacccgg cgtcgtccgt caacatggca gatgatcaaa atatctcagc
     6781 gcagatccgc ggcaaaataa agaattatgc tgcggcgggt aaaattcagc agggccggct
     6841 ggtggtcaac ggtgccagta tgccgcagag agttgaatcc gatggttcat ttgcacgccc
     6901 ttatattttc actgaaggca gcaacagcgt gcaggtcatc agcccggatg ggcaaagccg
     6961 acaaaaaatg cagttttact caacgccggg tacaggaacg attcgtgcac gtttacggct
     7021 ggttctctcg tgggatacgg acaataccga tctcgatctc catgttgtca cgcccgatgg
     7081 cgaacacgcc tggtacggta acaccgtgct gaaaaacagt ggtgcactgg atatggatgt
     7141 cacgacgggg tacggacccg agattttcgc catgccagcg ccagttcacg gacgctatca
     7201 ggtgta
//
LOCUS       DADLFA010000185         7201 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000185 DADLFA010000000
VERSION     DADLFA010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7201
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.187"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(235..789)
                     /locus_tag="JQH65_003469"
                     /pseudo
     CDS             complement(235..789)
                     /locus_tag="JQH65_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            932..1054
                     /locus_tag="JQH65_003470"
                     /pseudo
     CDS             932..1054
                     /locus_tag="JQH65_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1316..1882)
                     /locus_tag="JQH65_003471"
     CDS             complement(1316..1882)
                     /locus_tag="JQH65_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6140770.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYHISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(2164..2478)
                     /locus_tag="JQH65_003472"
     CDS             complement(2164..2478)
                     /locus_tag="JQH65_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6140771.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSAQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(2556..2666)
                     /locus_tag="JQH65_003473"
                     /pseudo
     CDS             complement(2556..2666)
                     /locus_tag="JQH65_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2990..3667)
                     /gene="ospD1"
                     /locus_tag="JQH65_003474"
     CDS             complement(2990..3667)
                     /gene="ospD1"
                     /locus_tag="JQH65_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspD1"
                     /protein_id="HAY6140772.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKRNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQSISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(4038..4175)
                     /locus_tag="JQH65_003475"
                     /pseudo
     CDS             complement(4038..4175)
                     /locus_tag="JQH65_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(4257..4555)
                     /locus_tag="JQH65_003476"
                     /pseudo
     CDS             complement(4257..4555)
                     /locus_tag="JQH65_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5144..5303)
                     /locus_tag="JQH65_003477"
                     /pseudo
     CDS             complement(5144..5303)
                     /locus_tag="JQH65_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(5307..6461)
                     /locus_tag="JQH65_003478"
                     /pseudo
     CDS             complement(5307..6461)
                     /locus_tag="JQH65_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(6481..6828)
                     /gene="tnpB"
                     /locus_tag="JQH65_003479"
     CDS             complement(6481..6828)
                     /gene="tnpB"
                     /locus_tag="JQH65_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAY6140773.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            complement(6828..>7201)
                     /locus_tag="JQH65_003480"
     CDS             complement(6828..>7201)
                     /locus_tag="JQH65_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY6140774.1"
                     /translation="RPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQY
                     RKGGLLPSGKNMPALLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRL
                     KGKLTPALLQILIREIKGSSH"
BASE COUNT         1858 a         1677 c         1554 g         2112 t
ORIGIN      
        1 aaaccttctc atgtttagag ttttttatta tgtttatcat aatgatatga cgatctgagt
       61 ttttaaaatc aagaagaaaa gataagtttt ttaaatgcac agtgcctgtg gataactttg
      121 cgtaaatccg gaggacagat cactctgaag caagacaaca gcatctgaaa acaatatgct
      181 cacgtttcaa ccacagggcg taagtgatcc tacccacgta atatggacac aggcctaagc
      241 gaggttctgg ttttcaaatt gttccggact gaggccgcca caccaactgt gccgtcgcca
      301 gcgattgtaa tcacattcga tataattaaa cactgttgcc cgcattattt cccggctgat
      361 aaagtgttct ccgtggagac attccacttt cagcgaatga aagaagcttt ccacgcaggc
      421 attatcgtag cagcagccct ttgcgctcat actgccacgc agattatgcc acttcagcag
      481 cgcctgataa tctgctgaac agtactgact tcctcggtcc gagtgaacga taacgttccg
      541 gggtctctta cgccgccaca gcgccatctg cagggcatcg caggccagtt gcgccgtcag
      601 gcgtggtgac atggaccagc caataacggc acgtgaccac aggtcaatga ccacagccag
      661 atacagccag ccttcatctg tacgtaagta cgtgatgtct cccgcccact tctggttcgg
      721 accactggcg taaaaatcct gctccagcag attttctgac acaggcaggc catgtgcgcg
      781 gtaactgacc gtattgctgg gtaagatcaa tttgctcact ggtatttact acgtgcatct
      841 aatgagtgat caagagccgt atgacgcgag agtgtcacgt acggttccgt gagcagccgg
      901 agagtgaaac tctctccggc tgactcgact cgcaggttcc gacagcggtg acgaacatgc
      961 agcggtgttg ttctcgctga tcggcacatg ccgtctgaac aatgtggagc cagaaaaatg
     1021 gctgcgttac gttattgaac atatccagga ctgaccggca aaccgggtac gcgatctgtt
     1081 gccttggaaa gttgatctga cttctcagta aatatcaata tgattctgac gagccgctta
     1141 cctttgagca gagaaagggg agagggatcc cacatccatg agctaggaaa atacaaaaac
     1201 tggctataca acaattcaat atattaggac ttacttgcga ggagaaatat agcaccgctc
     1261 aggcaattcc tgggtattta cttttcttag tttctttact cgggatgttc tgtcatcaga
     1321 aaggcgattc taaatttgta atataatcac tcatattccc acgtaatttc tttagatcaa
     1381 tatattgcac atttttctca aaaaactgat cggaaataag atttgcaaca ttgcttgttg
     1441 tatcattttt aacgcttcta ttcatccctt cacaaagttc gtccattttc tttttgaaat
     1501 aactgcttcc tgatacaaga tgatttatgg aactttgtgc agcattgatg tctagagaaa
     1561 tgtgataatc tttctcaacc tgtttaaata tctgtttacg gagattatcg ccgatttttc
     1621 ggctgtgttc tgcacaaaac ttatcaatac attggtttac cacccgggat acgtagactt
     1681 ttttttcatc atttttccaa tctgtaatat tttcaccgat ttgttctgaa acagagctca
     1741 aaatactagt atttattttg gttgatgata tttttactat ccttttcccc attgtacagc
     1801 ggataatctc atcaaccttt cctgaaaaat atcttttgtg agataaactg attccaaaat
     1861 tattaaaaga tgttccaagc attataatca ctttgtcatg taaaatagta acattgtcat
     1921 tattttagct gattcaaaca attctgtaaa tgctttgagt tctttactcc cttggctcaa
     1981 tgatggaccg tctggaatcc cagagactaa actctctgag aaagagagta tgaaaaaact
     2041 tggggcagtc cctaaatgaa gggaccaaca gcggtaaaat aaccggcatc attcataatg
     2101 atgccctgtt agttgtagtt atgaagattt acgtcattaa aattgctgtt cgtagtgtca
     2161 gtcctatggt ctggcatcag ctgaggatcg tagccgatac atcactggct tcacgacatt
     2221 cgtgttgagg ctattcatga aaactccacg ctgaaaacgc ctttttgtat aaacggtcac
     2281 ggtatgcccg gagccactgt tgctgatgaa ttcgataaaa ccttggcgtt tcttgaagct
     2341 atcgttaatg cggataatga aacaacgatt ggtgagattc gctcttttgc cgacaccctg
     2401 ggcgctgtcc ggttcaatcg caacaaaatc aaccggcagc tgagcaaacc caaccttgcc
     2461 tctctggcgc tggagcatga agtgatttag ctgggacgta gccactgaca gcttctataa
     2521 aagtatgatc ctacccacgt aatatgaaca caggcttaag tgaggttctg gttttcaaat
     2581 tgttcaggac tgagacctcc acaccaactg tgccgtcgtc agcgattgta atcgcacttg
     2641 atataattaa acactgtata tttcatttgt ggagtctcca gtctgagggg gctttatgca
     2701 ttccgtattg ggcactatga tgccagaaat ctgcaaggct ccacaccttc ctgtatttcc
     2761 cttcctctat cacagtgcac tttacttaat agggtgggat gtgttcatta catttattgt
     2821 gttttttatg attgtttttg cgtccagtgt cgttcgtcat gggaaactgg ttctatgggt
     2881 agtatcgctc agtctagtta actgtttgtt tctttttaga aattaagcag atcacacaat
     2941 tcggggagag ctccttctaa gtgcaattct ttgcagctat ttagcacact taaaacaact
     3001 cttgtatttt gtcagaaata atattaaaca tgtcttgttc attattgtta agtctgtctg
     3061 ctatttctgt aagttttatc ccactatgat ggcatatatt tatatatgcc atcatagcct
     3121 cgatattccc tttccctgcg gccagaaata atcctggtaa cccctcatta tctcttacat
     3181 ccaatagttt aatcgtttct tttggtgata aatcaaaagt tttaataaaa tctccataag
     3241 cttgaatcgc agcggaatga ccttcttgaa gagctatgaa caaagcagat tccccagcat
     3301 tatttcgtgc tgcaagaact tctagttttt cctctttggg tatttcggct gccaacagcg
     3361 attcaccgta tttacgcgct gcatcagcat ttccatgcca aatcaatgaa aacaaattat
     3421 ggcggttata ctccttatta ttctttgtcg gaatggatat gctctgtatt ttggccaatt
     3481 ttctgaaaga aacaccacta tatccccccc ctgatttttc ttcattgatg gcgtgggata
     3541 tagcggtatt tgtcacgtta atgatattac gtttcattcc tttattttca ttagcagtat
     3601 tgctgcctat tattaggtgc atatttttgt tgtttgctgg atgtaatcca tagttattta
     3661 ttgacatttt tgttcatcct ttttgctaaa aaaaacaata acctattttt gtaatgtaat
     3721 agatattagt ctattgctaa tctaaaaaaa ccattgccac attgaatggc tatattcacc
     3781 aataaattct ttcgatttcg tatattgaca tatcgtagat atatcttatc cctaagattc
     3841 aataatcgag tccgtttacc attctgtgca agcgccttca ctcagaagtg accgtcaagg
     3901 cgatcaccaa acttgataat aaagcagctc attgccatac gccagtcctt aaggggcgtt
     3961 gtccatttct gtgatgctgc ctagatttaa ctgcctgctc cggcttatct acatctaaat
     4021 atgtgagcct aataccgcta cactttggcc agactatgtt tttctccggg aagtgggcta
     4081 accgtctcca taaagtgacg aacttttttc aacagtggcc atattgaacg gcactgatga
     4141 ttacgggtta ttgtctcatg aagagccggc cacatcactg acagcagtga cttcaggcct
     4201 tcagcaccag acagcctgaa ccagttaatc catcgcccga cagatttagt aatctagggc
     4261 tggtttttct tcagctgttg cggttcgaaa gtgccattac gatcacgcag agtacggatt
     4321 tccagaggtt catctccagt agtgataatc ttcgttaagc aaccattgcg ggaattgctg
     4381 ccagatctaa gctgattttt ctcgcaccca agatgatgag tcatattagc attgagtacg
     4441 gtttcatttt cagcatccaa tcaaactgac tgagatcttc aggatttttg agacttttgg
     4501 ctagttcatt agctaaagcc cgcaacttat tagatgtcaa ctgtttttca ttcataaatt
     4561 caccttcatt tgatatcaaa ttaaatagtt caacatcaga gaattataca aacttatgta
     4621 caggctccta tgatcttcga atcctgaaga ttatagtgaa aataagtttt cacgttgaaa
     4681 tcccacatag ttagtatatg ataaataatc atggatgacc tgattaacct cttggggtag
     4741 tgcttttata gaagaataaa tattctacta atttaataca tcacctaaaa tagtattgtt
     4801 ttttatggtt ggttgaaggt cgtggtgatg atgttagtcc ttttgagaat attccagtgc
     4861 cagatcttac ttataataaa ggggcttggc agtttggata aactattgtt cttattccac
     4921 taatacaaac tttttgtaaa gtatattaaa tatacttcat ttcatactga aatccccgga
     4981 agtattcaat caaacaatta caccataaaa ggaagagggg gccctaatta agttgtcaag
     5041 catgtttatg atacctgcag ggtataaatt ttctggctgc aagcagacac atataaaacg
     5101 tctttattga tcctacccac ataatatgga cacagtcgta agtgaggctc tggttttcaa
     5161 attgttccgg actgagacca ccacaccaac ggtgccgtcg ccagcgattg taatcgcact
     5221 cgatataact aaacactgtt gcccgcacta tttcccgatg gataatagtg ttctccgtgg
     5281 agacattcca ttttcagcga atgaattttc atctgttgct gtatccagtc atacagtgac
     5341 tgcatcagtg gcgcggctct ggcttttctt gccgccagac gctgttctgc tgaacatccc
     5401 cggacttctg cctcgatggc atacagttca ccgatacgca gcagggcttc cgttattctg
     5461 ccggattcgt acaacgcccg gtaaccaccg taagcatcgg cctgaagcac tccgctgtaa
     5521 ccggccagat gattctgtgg atggatacct ttccggtccg gtgagtacgc gaaccagacc
     5581 gccggaggca tttccgaacc ggcgttacgg tcatcacgga catagaccca cagccgggca
     5641 gttcttgttt taccactgcc cggctcctgg accggaacgg ggatatcatc cgcatgaact
     5701 ttaccgggca tcagcacata ctggcgcagg acgtcataca gcggctccag cagttcagca
     5761 acggcaccgg tccagcgacc cagtgtggca cggctcagct ccacgccctg acgacgttat
     5821 atttctgact ggcggtataa cggcagatag cctgcatatt ttccagcgac aacatgggcc
     5881 agaagccccg ctccggcata actgcgtgca atgggttttg aaggcaccgg tgcctgcacg
     5941 atatggtcac accggcaaca ggccagtttc ggacgttgtg tttcgataac cttaaaggcg
     6001 ctgctgataa gctccagctg ctctgacaca tcacatccca gagaactgag ttcgccacca
     6061 caggccgggc agcattcctc ttccggacgg atgacccggg tttcacgggg aagtgaggca
     6121 ggtaacggtt tacgggctga agactggcgc aggggagatg gcagtaccgg gtcatattgc
     6181 tcacccagcg tttccgccat ttcttcctga agtgcgctga ttcgctcctg tgcttccagt
     6241 atctgccgtt cggtttttgc gcgaagtttt tctgagcttt aaccgaactg catgcgctgc
     6301 agtttcgcaa ccagcgcctt cagccggttg atttcggaag cataagccgc cacccgctgt
     6361 gagagcaggc ggttgtattt cgccatctgg cggatggtgt cctgttgcgt ctgcaacagt
     6421 gcccgcaggc gggcattctc atgagcaagt gaggtgtcca tatcctcact ttacaacggg
     6481 ttatatgcgg atgccagcgc gttccgttcg tttcgggtgt ttccagtcga tgccctcaag
     6541 aagcatggat aactgggccg gagtaaggtg caccttgccg tcacgggtga ccggccagac
     6601 gaagcggccc cgctccaggc gtctggtaaa gaggcacagt ccgtcactgt cagcccacaa
     6661 cacttttatc tggtcacccc ggcgtccgcg gaagatgaac agatgcccgg agaacgggtc
     6721 atccttcagg acgttctgaa cttttgaggc caggccgtta aagccatttc gcatatcggt
     6781 gatacctgca accagccaga tacgcgaacc tgcagggaaa gatatcatca gtggctgctc
     6841 ccttttattt cgcggataag tatctgtaat aacgccggcg tcagtttacc tttaagcctg
     6901 agagttccgg ccggcagaac cagctcacag cacagactgt cggacggtgt atttatctgc
     6961 tctggttcct gtgccggagc cgggatttta ttatccggct ccggcgttaa cgtcacggga
     7021 agcagtgcag gcatattttt tccggaaggc agcaggccac ctttccggta ttgatggcgc
     7081 cagttgaaga gcaggttatc gttgattccg ttttcacggg cgatctgcgc cacacaggct
     7141 ccgggctgca gtgactgctc cactaaggcg attttaaatt cataaggaaa attaggccgc
     7201 c
//
LOCUS       DADLFA010000186         7195 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000186 DADLFA010000000
VERSION     DADLFA010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7195
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.188"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(826..1356)
                     /locus_tag="JQH65_003481"
                     /pseudo
     CDS             complement(826..1356)
                     /locus_tag="JQH65_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            complement(1699..2349)
                     /gene="zinT"
                     /locus_tag="JQH65_003482"
     CDS             complement(1699..2349)
                     /gene="zinT"
                     /locus_tag="JQH65_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HAY6140775.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(2606..3241)
                     /gene="msrQ"
                     /locus_tag="JQH65_003483"
     CDS             complement(2606..3241)
                     /gene="msrQ"
                     /locus_tag="JQH65_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HAY6140776.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFNRLRKQVHNKLSV"
     gene            complement(3242..4246)
                     /gene="msrP"
                     /locus_tag="JQH65_003484"
     CDS             complement(3242..4246)
                     /gene="msrP"
                     /locus_tag="JQH65_003484"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HAY6140777.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(4355..4711)
                     /gene="hiuH"
                     /locus_tag="JQH65_003485"
     CDS             complement(4355..4711)
                     /gene="hiuH"
                     /locus_tag="JQH65_003485"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HAY6140778.1"
                     /translation="MVYAAQQNILSVHILNQQTGKPAADVTVTLEKKADNGWLQLNTA
                     KTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLESFFPEIPVEFHINKVNEHYH
                     VPLLLSQYGYSTYRGS"
     gene            4882..5553
                     /gene="hprR"
                     /locus_tag="JQH65_003486"
     CDS             4882..5553
                     /gene="hprR"
                     /locus_tag="JQH65_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HAY6140779.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            5553..6911
                     /locus_tag="JQH65_003487"
     CDS             5553..6911
                     /locus_tag="JQH65_003487"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HAY6140780.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHQALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDIASLGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSASYHYLNKHNVFRITLPQ
                     RN"
BASE COUNT         2021 a         1484 c         1627 g         2063 t
ORIGIN      
        1 gctgagaaaa aagcgtgctt tcccaatctg gggcaaatca cgtctgaatg acacctgaaa
       61 acaggtggtg gactcaagaa ggcttggaat attcttcttt aatgttatgt aattcattga
      121 tttttcgtgt acgattttaa gacatttttt ccaagaaaaa tttttaactc tttgattttt
      181 cgactctgta tcatcggtct cgaaaaccgg agtaggggca actctaccgg gggggcaaat
      241 ccccctctct ccgccacttt atcaatgact tatctcccga cttcccgcct tacttttcct
      301 aaacagaaca atcgtagaat attcttgaag ggttagatcg tcactgtttt ctgttcgata
      361 ctgtgacatt cagcacttga ttcgctatgg atctgacagg aagctttcga gcgaaaatgt
      421 gcagttattc agtcgttttc ttatcgctca ccattattct tttagacatt catcctatga
      481 aactgccgca aagttggtgg tgggaactga agttgcgtag agaaggggtc aataattgag
      541 tcaaaaaagg aacgcggccg tcagattaaa gccgcgggac aaagtccata atttccaaga
      601 gttgttgaat ttcccagctt ttttgaaagc ctttgtatca tactaacaag taaaaaaggt
      661 ttaaatacca aaattaacct gtatttttga taagtcttac cattgtacga tttacacagt
      721 ttctgctcct ccttgttgat agtggaaagg gtgcattgtg cacttgcgta aacatttatg
      781 acgcgttgat gtccatggtt cacatttcct tgtcagggga tcgacttatt tgcggcgagg
      841 catcattcgt aacagagtat tatctttctg aatgtagtga tgaaagagtg ctgcgccagc
      901 gtgagctgcg attaaaaagt aacctatatt tgcccaggtt tcgtgaatat tttatatcag
      961 tgctttaatt tcgctgtttg gggtaacaaa gggagacaca ttgaaaccaa ggaaactcca
     1021 cgattttcca ctgtaagcca tcaaagcaat ccccagcaga ggaagtgcaa ggaaggttat
     1081 ataaagcatg atgtgcatta gcgaagccgc tttcatttgc caggcaggtg gcggtggcac
     1141 aatagaagga tcactataac ggtgttttat aatcaggcgt gaaaacatta acacccaaac
     1201 gaatataccc gcattgtaat gtgtttctcg catcagcaga taagtactac tacctttagg
     1261 aaaccagcca cggagttcca tcgcggcata ggttattgca acgaaaagca gcgttatcca
     1321 atgtaaataa atttgagttt ttgagaatcg gttcattttt gtagctcctg taatttttac
     1381 agtcaagcta tcccacgaac cttaaaaatt ccttatacag atgtggtttt aagaatttgt
     1441 tatttaaaaa attgatagtg cacattgtaa ccagccaata taagcaatgg cgtgagcaca
     1501 ggtatctttc tctgttggcc gtattgtgta tggaatccgt tattggttaa ctcaatgcgg
     1561 gttagcttat taatatacag acacgtcata gtgatgagcg tgtctgtagc tagcttgcgt
     1621 tcagtggcgt ttgtggatgg accagtggca gttatgatga cttactaaag cggcatcgag
     1681 gcgttatcat gagaatactc aatgagacat catttcctcg accacttctt cgctactcaa
     1741 ctgatatgga taatacgttg gccagttttc catttcattt aataaagatt gctgtgaatc
     1801 attacccata aaaatgtgaa aatgagatga ttttcgtggt gcaattatat gatcgctaaa
     1861 ttgtatatat ttaggggctt tgctttcagg atctttacat tcgaataagt aacgaacgcc
     1921 tttcttgcct gatttatagg tgagtatttt gtatccatcg taatcatatt tacaggatgt
     1981 tgtttcatta tttctatgga attcaacaat gccgtcctca atgccaatca tctcgatatc
     2041 tgttgcataa cctttgtgat aataatcttt aatttcagca aatgttttag ttttatctgc
     2101 atccgctttc ttctgaaaga cggggtcaag tttgccactt tgcagtaaag gataaacgga
     2161 ttgccagacc ccatcccagt cactgagcgt tcggttttgt acattggcat catcaaaaac
     2221 accattagca gctttttgtt cgacctctgt taagggtttg ccgtgtgagt gatgaccatg
     2281 cgaaaaggca ggagcgctaa caataaagac acctaacgca acagccagtt tgtgaagacg
     2341 aatcgccaaa acagtttcct ccagagtcat tgtatatgat gtgtaatgtt atattgtaac
     2401 atatggcaaa atgcaacaac tcttaggaga tgagagcata gttttctcag gtgtacttat
     2461 ctatcttatt gaaataaaat gaattatatt gataatttga ggttgatgac aaacgaaaca
     2521 taatctgatg tgatctgcgt aggccggata gggcgctcgc gccgcatccg gcagggataa
     2581 cgcgtacttt gtcggcaata tgaagttata cagataattt gttgtgaacc tgcttgcgta
     2641 accggttaaa aagggaacgc aacttcttat accgtaaggc taaaagcagt acagccagcc
     2701 cagcgtagat gagcggctgc ggtgaaataa tcttcactga ccacagataa tgtattgggg
     2761 ccaggatcgc gacaagatag acgaaattat gcaactgttg ccagtgcttg ccgagttttc
     2821 gctgcatcgc ctgggttgaa gtaaacgcta aagcaagcaa aataacccag ctgataattc
     2881 ctaacgttaa ataaggccgg gtaattaact ccttgcctaa taacgccaga ttattcacgc
     2941 ccagctccag caatgcgtaa ctggttaaat gcagtgtcgc ccaggcaaag caccataagc
     3001 ctaacaggcg gcgagtgcgt atcaataacg gctgttttgc gtagcgtgcc agaggtgtga
     3061 ttaacaaggt cgccagcaaa aatttcagtg cagtgcgacc ggtaaaatgc tgaatatctt
     3121 tcaccggatc ggcacccagt ccaccgtgat tgatcgccca gaccagccag agaaacggca
     3181 acaatccggc aagatgcagg ctaactttca gccatgtcac ctgttttgct gtcagacgca
     3241 ttcagaaatt ctcccgcaaa tccaggccac gatacagcga tgccacctgg tcggcgtaac
     3301 cattaaacag tagcgttggc tggcgctgaa catcgaggat gccgcctgaa ccaataaatc
     3361 gttcggtagc ctgtgaccag cgcgggtgat caacatgcgg attaacgttg gcgtaaaaac
     3421 cgtattcgtc aggcgctgcc agattccagg tggttggcgg acgctcgcgg gtcagcttaa
     3481 tactgactat cgatttaatc cctttaaagc catatttcca cggcacaatc agtcgtaccg
     3541 gcgcgccatt ttgtggcggt aacgccttgc cataaacacc cacggtcatc agtgtgagcg
     3601 gatgcattgc ttcgtcgaga cgcaatcctt cgacataagg atatttcagc ccgccgccga
     3661 taaagcggtc ctgctggcct ggcatttgtt ccggtgcata aattgtttcg aaagcgacat
     3721 acttcgcatt gctggtgggt tcggcaagcg ccagcaattt gtgcagcgga aaaccaatcc
     3781 acggcaccac catcgaccat gcctccacgc agcgcatacg ataaatacgc tcttccagcg
     3841 ggaagcgacg ggttaaatca tcgtgatcga gggtcaatgg ttttgccact tcgccgctga
     3901 ttttcagtgt ccatggatcg gttttcaggc taccagcatt agcggcggga tcggctttat
     3961 ccagcccgaa ttcatagaag ttgttataac cggagacttt atctactggc gtcagtggca
     4021 ggttattttg ccaggcggca ggcttgctga actccagcgg ttttccggcg ggggcgggcg
     4081 ggcgatcgtt ccctttaaac cagctaagca gatcggcatg cgcagcgtga ggcaaagaaa
     4141 gtgcagctgc actgatgccc agtgctttta acacctgccg acgcttcata aagaataccg
     4201 actcggccgt aacatctgat tcttttaaaa attgattctt tttcataagc catcacactt
     4261 ttttgtcgtt agccagcaag agccgtttac tgttaatctg tcagggagtg tcctccctaa
     4321 cagtagcaga gatctgcttt tacatattta tgcattaact gccacgatag gttgaatacc
     4381 catattggct aagtaataaa ggcacatgat aatgctcgtt cactttatta atatgaaatt
     4441 caacggggat ctcagggaag aaactttcaa gattttgttt cttgaaatag tccccggttt
     4501 taaatacgac acggtaatcg cccgtagttg cagtttgctc gggccacagt gctttaattc
     4561 gtccatcctt atctgttttg gcggtattaa gttgtaacca gccgttatcc gccttctttt
     4621 caagagtgac tgtcacgtca gcagcgggtt ttccggtttg ctggttcaaa atgtgcacgc
     4681 taagaatgtt ttgttgtgcg gcataaacca aagaaggtaa tgaaaatgct gcaaataacg
     4741 ctttaacatg tttatatcct tgtcatgtga atgagttttg aggatagcga aaaagcggcc
     4801 agggtaacct tacagcagca ttacaatttt gtaatgaacc aggctgtttc tataacatat
     4861 gatttatggc atattatttt catgaagatt ttacttattg aagataatca aaggacccag
     4921 gagtgggtaa cgcaggggct ttccgaagcg ggttatgtca ttgatgccgt ttctgatggc
     4981 agagatgggc tttatcttgc gctgaaggat gattatgcat tgataattct ggatattatg
     5041 cttccgggta tggatggctg gcagatctta caaacgttaa gaacagcaaa gcaaacccct
     5101 gttatttgcc ttactgcaag ggattctgtc gatgacagag tcagagggct ggacagtgga
     5161 gcaaatgatt atctggtaaa acctttttca ttttctgagt tgctggcaag ggttcgggca
     5221 caattaaggc aacatcacgc tttgaattca acattagaaa tcagtggctt aagaatggac
     5281 tctgtaagtc aaagtgtgag cagggacaat atcagtatta cactgacgcg caaggagttt
     5341 cagttacttt ggctactggc ctccagagct ggcgaaatta tacccagaac ggttattgcg
     5401 agtgaaattt ggggaatcaa ctttgatagt gataccaata cggtggatgt cgccattcgc
     5461 aggctccgcg caaaagttga tgatcctttt cctgaaaagc taatcgccac aatccggggg
     5521 atgggctatt cattcgtagc ggtaaaaaaa taatgaaaag attgtctata accgtccgtt
     5581 taaccttgct ttttatattg ctgctgtctg ttgctggcgc tggaattgtc tggactctct
     5641 ataatggcct ggcaagtgag ttgaaatggc gcgatgatac aacactcatt aaccggacag
     5701 cacagatcaa gcagttgtta attgatgggg taaatccaga tacgttacct gtatacttta
     5761 accggatgat ggatgttagt caggatatct tgatcattca tggtgatggc atcaataaaa
     5821 ttgttaaccg gacaaatgtc agtgatgaca tgttaaataa catacctgct agtgagacaa
     5881 tcagcgcagc tggcatttac agaagcatta ttaatgatac tgagattgat gctttacgaa
     5941 ttaatattga tgaagtttcg ccatcattaa cggttactgt ggctaaattg gcttcagcta
     6001 gacataacat gcttgaacag tataaaatta atagcattat aatttgcatt gtcgccattg
     6061 tactttgctc agtattaagt ccgctgttaa tcagaacggg attacgagag atcaaaaagt
     6121 tgagtggtgt aacggaagcg ctgaattata acgatagccg agagcctgtt gaggttagcg
     6181 cattaccgag agaactaaaa cctcttgggc aggcgttgaa taaaatgcat caagccttag
     6241 tcaaagattt tgaacgccta agtcaatttg ctgacgatct cgctcatgaa cttagaacgc
     6301 ccattaatgc attactgggt cagaatcagg ttacgctcag tcaaaccaga agtatcgctg
     6361 aatatcaaaa aacaattgcc ggtaacattg aagagttgga aaatatttcg cggttaacag
     6421 agaacatact gtttcttgcc cgggcagata aaaacaatgt tttggtgaaa ctggactcgc
     6481 tttctctcaa taaggaagtc gaaaatttgt tggattatct tgaatatctt tcagacgaga
     6541 aagagatttg ctttaaggtc gagtgcaatc agcaaatctt tgcggataaa attttactgc
     6601 aacgaatgtt atcgaatctt attgttaatg ccattagata ttcaccagaa aaatcgcgta
     6661 ttcatataac cagttttctt gataccaacg gctatcttaa tattgatatc gccagtcttg
     6721 gaacgaaaat acatgagcct gaaaaactct tccgtagatt ttggcgggga gataattcgc
     6781 gtcattccgt aggtcaggga cttggtcttt ctttagtcaa agcgattgcc gaattgcatg
     6841 ggggaagtgc ttcatatcac tatctcaata agcataatgt gttccgaatt acgttaccgc
     6901 aaagaaatta acatagggct tcagtacgcc ataaattaac cgtgaaaaaa cgcagccacg
     6961 tcgtgagtac taacgcggct gcgattaatt atgcgcttac attcaaacgt aacagggatt
     7021 aacccgcgta agctgccagc atttcctgcg ccgccagttt acccaacgca ttcgctgcga
     7081 aaggactgtc gccggtgaga agtttacggt ccttatgtac tcgcccggtg atgtcgtcat
     7141 taatgatgtt catgcccatt ttcttcagct cttcgccgaa gtaccaggtg agatg
//
LOCUS       DADLFA010000187         7120 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000187 DADLFA010000000
VERSION     DADLFA010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7120
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.189"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..648
                     /locus_tag="JQH65_003488"
     CDS             <1..648
                     /locus_tag="JQH65_003488"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HAY6140781.1"
                     /translation="TYKYADMLAMSAKKDAMVPMGGLLCMKDDSFFDVYTECRTLCVV
                     QEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYRIAQVQYLVDGLEEIGVVCQQAGGH
                     AAFVDAGKLLPNIPADQFPAQALACELYKVAGIRAVEIGSFLLGRDPKTGKQLPCPAE
                     LLRLTIPRATYTQTHMDFIIEAFKHVKENAANIKGLTFTYEPKVLRHFTAKLKEV"
     gene            739..1986
                     /gene="tnaB"
                     /locus_tag="JQH65_003489"
     CDS             739..1986
                     /gene="tnaB"
                     /locus_tag="JQH65_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HAY6140782.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            2118..3293
                     /gene="mdtL"
                     /locus_tag="JQH65_003490"
     CDS             2118..3293
                     /gene="mdtL"
                     /locus_tag="JQH65_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HAY6140783.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVTDRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            3268..4227
                     /gene="yidZ"
                     /locus_tag="JQH65_003491"
     CDS             3268..4227
                     /gene="yidZ"
                     /locus_tag="JQH65_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HAY6140784.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            4384..5133
                     /locus_tag="JQH65_003492"
     CDS             4384..5133
                     /locus_tag="JQH65_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6140785.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            5155..5721
                     /gene="yieF"
                     /locus_tag="JQH65_003493"
     CDS             5155..5721
                     /gene="yieF"
                     /locus_tag="JQH65_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent chromate oxidoreductase YieF"
                     /protein_id="HAY6140786.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDRQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(5775..7112)
                     /locus_tag="JQH65_003494"
     CDS             complement(5775..7112)
                     /locus_tag="JQH65_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAY6140787.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
BASE COUNT         1693 a         1821 c         1802 g         1804 t
ORIGIN      
        1 acctacaaat atgccgatat gctggcgatg tccgccaaga aagatgcgat ggtgccgatg
       61 ggcggcttgc tgtgcatgaa agacgacagc ttctttgatg tgtacaccga gtgcagaacc
      121 ctttgcgtgg tgcaggaagg cttcccgaca tatggcggcc tggaaggcgg cgcgatggag
      181 cgtctggcgg taggtctgta tgacggcatg aatctcgact ggctggctta tcgtatcgcg
      241 caggtacagt atctggtcga tggtctggaa gagattggcg ttgtctgcca gcaggcgggc
      301 ggtcacgcgg cattcgttga tgccggtaaa ctgctgccga atatcccggc agaccagttc
      361 ccggcacagg cgctggcgtg cgagctgtat aaagtcgccg gtatccgtgc ggtagaaatt
      421 ggctctttcc tgttaggccg cgatccgaaa accggtaaac aactgccatg cccggctgaa
      481 ctgctgcgtt taaccattcc gcgcgcaaca tatactcaaa cacatatgga cttcattatt
      541 gaagccttta aacatgtgaa agagaacgcg gcgaatatta aaggattaac ctttacctac
      601 gaaccaaaag tattgcgtca cttcaccgca aaactgaaag aagtttaatt aatactacag
      661 agtggctata aggatgttag ccactctctt accctacatc ctcaataaca aaaatagcct
      721 tcctctaaag gtggcatcat gactgatcaa gctgaaaaaa agcactctgc attttggggt
      781 gttatggtta tagcaggtac agtaattggt ggaggtatgt ttgctttacc tgttgatctt
      841 gccggtgcct ggtttttctg gggtgccttt atccttatca ttgcctggtt ttcaatgctt
      901 cattccgggt tattgttatt agaagcaaat ttaaattatc ctgtcggctc cagttttaac
      961 accatcacca aagatttaat cggtaacacc tggaacatta tcagcggtat taccgttgcc
     1021 ttcgttctct atatcctcac ttatgcctat atctctgcta atggtgcgat cattagtgaa
     1081 acgatatcaa tgaatttggg ctatcacgct aatccacgta ttgtcgggat ctgcacagcc
     1141 attttcgttg ccagcgtatt gtggataagt tcgttagccg ccagtcgcat tacctcattg
     1201 ttcctcgggc tgaagattat ctcctttgtg atcgtgtttg gttctttctt cttccaggtc
     1261 gattactcca tcctgcgcga tgccaccagc accactgcgg gaacgtctta cttcccgtat
     1321 atctttatgg ctttgccggt gtgtctggcg tcatttggtt tccacggcaa tattcccagc
     1381 ctgattattt gctatggaaa acgcaaagat aagttaatca aaagcgtggt atttggttcg
     1441 ctgctggcgc tggtgattta tctcttctgg ctctattgca cgatggggaa tattccgcgc
     1501 gaaagcttta aggcgataat ctcctcaggc ggcaacgttg attcgctggt gaaatcgttc
     1561 ctcggcacca aacagcacgg cattatcgag ttttgcctgc tggtgttctc taacttagct
     1621 gttgccagtt cgttctttgg tgtcacgctg gggttgttcg attatctggc ggacctgttt
     1681 aagattgata actcccacgg cgggcgtttc aaaaccgtgc tgttaacctt cctgccacct
     1741 gcgttgttgt atctgatctt cccgaacggc tttatttacg ggatcggcgg tgccgggctg
     1801 tgcgccacca tctgggcggt cattattccc gcagtgcttg caatcaaagc tcgcaagaag
     1861 tttcccaatc agatgttcac ggtctggggc ggcaatctta ttccggcgat tgtcattctc
     1921 tttggtataa ccgtgatttt gtgctggttc ggcaacgtct ttaacgtgtt acctaaattt
     1981 ggctaaatcc ttcaagaagc cagccattcg ctggcttctt gcctctcagg aaatcactta
     2041 tgtccaaatg gcaactcgcc tgatcctcct tcaccacgta tgctttgcgt caccttacta
     2101 tcaggacgct ttagcccatg tcccgctttt tgatttgtag ttttgccctg gttttacttt
     2161 atcccgccgg gattgatatg tacctcgttg gtttaccgcg catcgccgcc gatctcaatg
     2221 ccagcgaagc gcagttgcat attgcgttct ccgtatatct ggcggggatg gcagctgcga
     2281 tgttatttgc cggtaaagtg accgatcgtt cagggagaaa gccggtcgcc atacccggcg
     2341 cggcgctatt tattattgcc tcggtgttct gttcactggc tgaaaccagc acgttatttc
     2401 ttgcaggccg atttctacag gggttgggcg caggctgttg ttacgtagtg gcgttcgcca
     2461 ttttgcgcga cacgctggat gatcgacgtc gggctaaagt gctgtcatta ctcaacggta
     2521 ttacctgcat cattccggtg ttagcgccag tgctcggaca tctgattatg cttaaattcc
     2581 cgtggcagag tctgttctgg gcgatggcaa tgatgggcat cgcggtactg atgttgtctt
     2641 tgtttatttt aaaagaaacg cgcccagcgt cccccgctgc ttcggacaaa ccacgagaaa
     2701 atagcgagtc gctgcttaat cggtttttcc tcagccgtgt tgttatcacc accctcagcg
     2761 tttcggtgat cctcactttc gtcaatacat cgccggtatt gctgatggaa atcatgggtt
     2821 ttgagcgcgg agaatacgcc accattatgg cgttgaccgc tggcgtcagc atgaccgttt
     2881 cattctccac gccatttgcg ctgggaattt ttaagccacg tacgttgatg atcacctcgc
     2941 aggtgttatt ccttgcagcg gggatcaccc ttgccgtttc accttcccat gcggtttctc
     3001 tgtttggtat cacactgatt tgcgccggtt tctcggtagg ttttggcgta gcgatgagtc
     3061 aggcgttagg accattttca ttacgcgcgg gcgtagccag ctcgacctta ggtattgcgc
     3121 aggtttgcgg ttcgtcactg tggatttggc tggcagcggt ggttggtatc ggcgcatgga
     3181 atatgctgat cgggattctg attgcctgta gcatagtgag cctgttgctg attatgttcg
     3241 tcgcgcctgg acgccccgtt gccgctcatg aagaaatcca tcaccacgct tgatctcaat
     3301 ctgctgctct gtctgcaact gctgatgcag gagcgcagcg tgaccaaagc ggcgaagcgg
     3361 atgaacgtga ccccttcagc ggtgagtaag tcgctggcaa agttaagagc gtggtttgac
     3421 gacccgctct ttgtgaactc accgctgggg ctgtcgccca caccgctaat ggtcagcatg
     3481 gagcaaaatc tggcggaatg gatgcaaatg agcaatctgc tgctggataa accgcaccac
     3541 cagacaccgc gcggcctgaa gtttgagttg gcggcggaat cgccgctgat gatgatcatg
     3601 cttaatgcgc tgtcgaaacg gatctaccaa cgttacccgc aggcgaccat caaactacgt
     3661 aactgggatt acgattcctt agatgccatt actcgtggtg aagtggatat tggtttttcc
     3721 ggtcgcgaaa gccatcctcg ctcgcgggag ctgttaagct cgctaccgtt agccattgat
     3781 tatgaagtgc tgtttagtga tgtaccctgt gtctggttac gcaaagatca tccggcactg
     3841 catgaaacgt ggaatctgga caccttttta cgttatccgc atatcagcat ttgctgggaa
     3901 cagagcgaca cctgggcgct ggacaatgtg ttacaggagc tggggcgcga acgcacgatt
     3961 gctatgagcc tgccggaatt tgagcagtca ctgtttatgg cagcgcaacc cgacaatctg
     4021 ctactggcga ccgcgccgcg ctactgtcag tactacaatc aactccatca actgccgttg
     4081 gttgctcttc ctctcccgtt tgacgaaagc cagcaaaaaa agctggaagt tccttttacc
     4141 ctgctgtggc ataaacggaa cagtcataat ccgaagatcg tctggttacg ggaaaccatt
     4201 aaaaaccttt acgcgtcgat ggcataaccg aatcgtatga aacgcgaccc aatttcacaa
     4261 taaaatgtaa aaaagttgta ataagcttgt ctgaatcgaa cttttagccg ctttagtctg
     4321 tccatcattc cagtaaatga ttactcttat attcataatg gaccattaag catggagcga
     4381 aaaatggcga ctcactttgc ccgagggatt ttaacggaag gacatctgat ttctgttcgt
     4441 ctcccctccc agtgtcatca agaagcccga aacattcccc ctcatcgtca aagccgtttt
     4501 ctggcgtcca gaggtttact cgcagaactg atgttcatgc tgtatggcat tggcgaattg
     4561 ccggaaatcg tcaccctgcc gaaaggtaaa ccggttttca gtgataaaaa tttgccttcg
     4621 ttttctattt cctatgccgg gaatatggtt ggcgtggcgt taacaaccga aggtgaatgt
     4681 ggcctcgata tggaactaca gcgtgcgacg cgcgggtttc atagcccaca cgcgcccgat
     4741 aaccacacct tttccagcaa tgaatcgcta tggatcagta aacaaaacga tcctaacgaa
     4801 gcgcgggcgc agctcatcac gctgcgccga agcgtgctaa aactaaccgg tgatgttttg
     4861 aatgacgatc cgcgcgatct gcagctgctg cccattgccg gacgcctgaa atgtgctcat
     4921 gtaaatcatg tagaagcgtt atgcgacgcg gaggacgtgc tggtgtggtc cgtggcggtc
     4981 acgcccgcga ttgaaaagct cagtgtctgg gagttagatg gcaaacacag ctggaaaagc
     5041 ctgccggata ttcacagccg cgccaacaat cctaccagcc ggatgatgcg ttttgcccag
     5101 ctctctaccg tgaagtcttt ttcgccaaat tgatagacaa ccacaggagt cattatgtct
     5161 gaaaaattgc aggtggttac gttactgggg agcctgcgca aaggctcatt taatggcatg
     5221 gttgcacgta ccctgccgaa aattgctccg gcgagcatgg aagtcaatgc gttaccttcc
     5281 attgccgaca ttcccctgta tgacgctgac gtacagcagg aagaaggttt tccagcaacg
     5341 gttgaagctc tggcggaaca gatccgtcag gctgacggtg tggtgatcgt cacgccggaa
     5401 tataactact cggtaccggg tgggctgaaa aatgccatcg actggctttc ccgcctgccg
     5461 gatcaaccgc tggccggtaa accggtgttg attcagacca gctcaatggg cgtgattggc
     5521 ggcgcgcgct gtcagtatca cctgcgccag attctggttt tcctcgatgc aatggtgatg
     5581 aacaagccgg aatttatggg cggcgtgatt cagaacaaag ttgatcggca aaccggagaa
     5641 gtgattgatc agggtacgct ggaccacctg accgggcaat tgaccgcatt tggtgagttt
     5701 attcagcgag ttaagatcta aataaaaaac ccgccagtaa tcatgcatgg cgggttttta
     5761 atgcgctatc gattttaatg agcgtcgata aatacaatct tcaggataaa cagcagcgca
     5821 acgatgatta cgcacgggct aaggtcacgc agacgcccgg taccaatctt catcacgcag
     5881 taggagataa agcccagcgc aataccttcg gtaatcgaaa agctgaacgg catcatcacg
     5941 gcggtaataa acgccggaac agattcagta agatcctgcc agttcacgcg agcaagactt
     6001 gaggtcatca acacgccaac gtagatcaac gcgccagctg cagcgtagcc tggcaccatt
     6061 cccgccagcg gcgacagaaa gataaccagc aggaacagca gaccaacaac cactgccgtc
     6121 agaccggtac gaccgccaac cgatacgccg gaagaggact caatataagc cgtaacggaa
     6181 gaagtaccga taaacgaacc ggtcacggaa gagatactgt cgacatacag cgcttgcttc
     6241 atgcgcggga atttcccctt ctcatccgcc agacctgctt tatcggtcac gccaatcagc
     6301 gtaccggagg agtcaaacaa gttgaccaac atgaaagaga aaatcacccc tgccagcccg
     6361 aggttaaacg acccggctaa atctacatga cccacaactg tcattacgct cggcggcgca
     6421 gaaacgatgc cattgtagtg cacatcaccc agcatccagc ccagcagcgt cgtcaccacg
     6481 atagaaacca gcaccgctgc gtgaatgttg cgcgaggcca gaatagcaat gatgaagaag
     6541 ccgaggatac ccagaagtac gctgtgagaa gtcagattac cgatgctcac cagcgtttcc
     6601 gagttagcga caatcacacc tgcgtttttc agccccatca tgccgataaa caggccgata
     6661 ccgctggtaa tacccacacg cagacttacc ggaatgttag caatcatcca gtaacgaacg
     6721 cggaaaatgg tcaaaagaag cagtccgaca gcgccccaga agattgcgcc cataccgatc
     6781 tgccacggca gccccattgc ctggacaacc acaaaagcga aaaaggcgtt aagccccatc
     6841 gcaggagcca gcgccaccgg aaggtttgca aacaatccca tcataatgct accgaaagca
     6901 gcaatcagac aggttgttac gaagacggca ctggtgtcca tgccagcaac gcccagaatt
     6961 tgtgggttaa caaaaacgat gtaaaccatc gtcaggaagg tggtaaaacc ggcgatcact
     7021 tcggtccgtg ccgtcgtgcc atgttcgcgc agtttaaaca cgcgttccag catcccctgg
     7081 ccagaagcct gggttgtgtg ttgttgactc attatctatt
//
LOCUS       DADLFA010000188         7056 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000188 DADLFA010000000
VERSION     DADLFA010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7056
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.190"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            40..246
                     /locus_tag="JQH65_003495"
                     /pseudo
     CDS             40..246
                     /locus_tag="JQH65_003495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            991..2904
                     /locus_tag="JQH65_003496"
                     /pseudo
     CDS             991..2904
                     /locus_tag="JQH65_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001306477.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3032..3754)
                     /locus_tag="JQH65_003497"
     CDS             complement(3032..3754)
                     /locus_tag="JQH65_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HAY6140788.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     LTNTFKLGPGVDVNYMLHTQNK"
     gene            3901..6528
                     /gene="gyrA"
                     /locus_tag="JQH65_003498"
     CDS             3901..6528
                     /gene="gyrA"
                     /locus_tag="JQH65_003498"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HAY6140789.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            6677..>7056
                     /locus_tag="JQH65_003499"
     CDS             6677..>7056
                     /locus_tag="JQH65_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 family protein"
                     /protein_id="HAY6140790.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIVLLSAGVWALQYAGSGPEKTLSP
                     LVMHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLD"
BASE COUNT         1693 a         1743 c         2013 g         1607 t
ORIGIN      
        1 ggctatggag atggtccttc cactgcggcg ggtggcttta tgtatctcgg cttaagtgaa
       61 gttacctttg atattgccga cggaaaaacg ctggttatcg gcaatacaga gaatgacgga
      121 gctgttgact ctattgctgg taccgggttg atcaccaaaa caggttccgg cgatctggta
      181 cttaatgcag ataacaatga ctttactggc gagatgcaga ttgaaaacgg tgaagttacc
      241 ctgggccgca gcaactccct gatgaatgtc ggcgatacgc attgccagga cgatccgcaa
      301 gactgctacg gtctgacgat agggagtatt gataagtacc agaatcaggc agagctaaac
      361 gttggctcca cccaacaaac ctttgtgcac tcattgacgg gctttcagaa tggcacttta
      421 aatatcgatg ctggtggcaa tgttactgtt aatcaaggta gttttgctgg caccatcgaa
      481 ggtgctggtc agctcaccat tgcgcaaaac ggcagctacg tgctgtcggg ggcgcagtcg
      541 atggcgctaa ccggcgatat agtcgttgat gatggtgcgg tgctttcgct ggaaggcgac
      601 gcggcagatc tcgccgctct ccaggacgat ccgcagtcga tcgtgttaaa cggcggtgtg
      661 ctcgatctct ctgatttctc cacctggcag agcggcacat catacaatga tggccttgaa
      721 gtcagtggca gcagcggaac ggttatcggc agtcaggatg tggtagatct tgcaggtggc
      781 gacaatttgc atatccgcgg cgacgggaaa gatggcgtct acgtggtggt cgatgcgagc
      841 gacgggcagg taagtctggc aaacaataat agttatttgg gcacaacaca aatcgcctcc
      901 ggtacgctga tggtgagcga caactcgcag cttggagata cccactataa ccgccaggtt
      961 atctttaccg ataagcaaca agaaagcgtg atggagatta ctgccaatgt cgatactcgc
     1021 tctacgacga ctgagcatgg gcgtgatatt gaaatgcgcg ccgacggtga agtggcagtt
     1081 gatgcggggg tagacacgca gtggggcgca ctgatggctg acagcagcgg gcagcatcag
     1141 gatgagggta gcacatttac taaaacgggg gcgggtacgc tggagctgac cgccagcggt
     1201 acaacgcagt cggcagtacg tgtcgaagaa ggcaccctga aaggcgatgt tgcggatatc
     1261 tttccttatg cttcgtcact gtgggtcggt gatggggcga cgttcgttac tggcgcggat
     1321 caggatattc agtctatcga tgctacttcc agcggcacta tcgatatcag cgatggtacg
     1381 gttttgcgtc tgaccgggca ggatacttcc gtcgccctta atgcctcatt gtttaacggc
     1441 gatgggacgc tggtgaatgc taccgatggc gtgacgttga caggtgagct taataccaac
     1501 cttgaaactg acagcctgac ctatcttgcc gacgtgacgg ttaatggtga tctgaccaat
     1561 acgtccggtg cggttagcct gcaaaatggc gtcgctggcg atacgctaac ggtaaacggt
     1621 gattatactg gcggcggtac gctactgttc gacagcgaat taaacggcga tgactcggta
     1681 agcgatcaat tggtgatgaa cggtaatact gctggcaaca caactgtggt ggttaactcc
     1741 attacaggga ttggtgagcc gacatcgaca ggcattaaag tggttgattt cgcagctgat
     1801 cccacgcagt ttcaaaacaa tgcacagttc agtctggctg gcagtggcta cgtcaacatg
     1861 ggagcgtatg actacacgct agtggaagat aacaacgact ggtatctgcg atcgcaagaa
     1921 gtaacgccgc catcgccacc tgatccagac ccggacccaa cacccgatcc tgacccaaca
     1981 cccgatccaa tccccgctta ccagccggtg ctgaatgcca aagttggcgg ttatctcaat
     2041 aacctgcggg cggcaaatca ggcgtttatg atggagcgac gcgatcacgc tggtggcgat
     2101 ggtcagacgc tgaatttacg tgttatcggc ggacgttatc attacacggc ggcagggcaa
     2161 ctggctcagc aggaagacac ttctacggtg cagcttagcg gcggcttgtt tagcgggcgc
     2221 tggggcacgg atggcgagtg gatgcttggg gcggttggtg gctacagtga taatcagggc
     2281 gacagccgct cgaatatgac cggaactcgc gccgataacc agaaccacgg ttatgccgtt
     2341 gggctgacct caagctggta tcagcacggt aattagaagc agggggcctg gctggatagc
     2401 tggctgcaat acgcgtggtt taacaatgat gtttccgaac atgaagatgg cgtggatcgt
     2461 taccattcgt cggggattat cgcctcgctg gaagccgggt atcagtggtt accggggcgt
     2521 ggtgtggtga ttgaaccgca ggcgcaggtg atttatcagg acgtgcagca ggatgatttt
     2581 accgccgcta accatgcgcg cgtgtcacaa tcgcaggggg atgatattca gacgcggctg
     2641 ggtttacaca gcgaatggcg taccgcggtt ggtgtcacac caacattaga tctgaattat
     2701 tatcacgatc cccatgcgac ggaaattgaa gaagatggca gcactatcag tgacgatgcg
     2761 gtgaagcgac ggggtgaaat aaaagtggga gtcacgggca atatcagtca gcgagtgtcc
     2821 ctgcgcggca gcgtggcgtg gcagaaaggg agtgatgatt ttgcccagac ggcagggttt
     2881 ttgtcgatga cggtgaaatg gtaatcactg tctagtcggc acggttgccg gatgcggcgt
     2941 aaacgcctta tccggcctac ggggagcatt tgtaggcctg ataagacgcg gcaagcgtcg
     3001 catcaggcat tggctcggcc aaacaacaac ctcacttatt ctgcgtgtgc agcatatagt
     3061 tcacatccac gccggggccg agtttaaaag tattagtgag cgggttgtaa tgtagcccag
     3121 ttatatgtcg ctctttaagg cttgtctgat ctacccagcc aagcagttct gccggtttaa
     3181 taaacttctt cacatcatgc gtgcctttgg gcaccatgcg caaaatatat tccgcgccaa
     3241 ccaccgccat cagccatgac ttgccgttgc ggttaagcgt cgagaaaaag acatcgccgc
     3301 ctggtttcac cagttgcgca caggctctga ccactgactg cggatcgggg acgtgctcca
     3361 gcatctccat gcaggtcacc acatcatact gcccggcatg ttttgccgcg tgctcttcca
     3421 cggtttcctg cacataatcc acctgaatgc cgctttccag cgcgtgcagt tttgccacct
     3481 gcaatggctc aaagcccata tccagaccgg tcaccgtcgc gccttcgcgc gccatactct
     3541 cggccagaat gccgccgcca caaccgacat cgagcacctt tttgccaaat aaaccgccag
     3601 cacgctcggc aatatagccc agacgcagcg ggttaatgcg gtgcaacggt ttgaactcac
     3661 cttcaagatc ccaccagcgg gaggcgacgg cttcaaattt agcgatctct tcgtggtcta
     3721 cgttatggtt ttccggcgat ttttcggcat tcattggcac ttctactccg taattggcaa
     3781 gacaaacgag tatatcaggc attggatgtg aataaagcgt ataggtttac ctcaaactgc
     3841 gcggctgtgt tataatttgc gacctttgaa tccgggatac agtagaggga tagcggttag
     3901 atgagcgacc ttgcgagaga aattacaccg gtcaacattg aggaagagct gaagagctcc
     3961 tatctggatt atgcgatgtc ggtcattgtt ggccgtgcgc tgccagatgt ccgagatggc
     4021 ctgaagccgg tacaccgtcg cgtactttac gccatgaacg tactaggcaa tgactggaac
     4081 aaagcctata aaaaatctgc ccgtgtcgtt ggtgacgtaa tcggtaaata ccatccccat
     4141 ggtgactcgg cggtttatga cacgatcgtc cgtatggcgc agccattctc gctgcgttac
     4201 atgctggtag acggtcaggg taacttcggt tccatcgacg gcgactctgc ggcggcaatg
     4261 cgttatacgg aaatccgtct ggcgaaaatt gcccatgaac tgatggccga tctcgaaaaa
     4321 gagacggtcg atttcgttga taactatgac ggcacggaaa aaattcccga cgtcatgcca
     4381 accaaaattc ctaacctgct ggtgaacggt tcttccggta tcgccgtagg tatggcaacc
     4441 aacatcccgc cgcacaacct gacggaagtc atcaacggtt gtctggcgta tatcgatgat
     4501 gaagacatca gcattgaagg gctgatggaa cacatcccgg ggccggactt cccgacggcg
     4561 gcaatcatta acggtcgtcg cggtattgaa gaagcttacc gtaccggtcg cggcaaggtg
     4621 tatatccgcg ctcgcgcaga agtggaagtt gacgccaaaa ccggtcgtga aaccattatc
     4681 gtccacgaaa ttccgtatca ggtaaacaaa gcgcgcctga tcgagaagat tgcggaactg
     4741 gtaaaagaaa aacgcgtgga aggcatcagc gcgctgcgtg acgagtctga caaagacggt
     4801 atgcgcatcg tgattgaagt gaaacgcgat gcggtcggtg aagttgtgct caacaacctc
     4861 tactcccaga cccagttgca ggtttctttc ggtatcaaca tggtggcatt gcaccatggt
     4921 cagccgaaga tcatgaacct gaaagacatc atcgcggcgt ttgttcgtca ccgccgtgaa
     4981 gtggtgaccc gtcgtactat tttcgaactg cgtaaagctc gcgatcgtgc tcatatcctt
     5041 gaagcattag ccgtggcgct ggcgaacatc gacccgatca tcgaactgat ccgtcatgcg
     5101 ccgacgcctg cagaagcgaa aactgcgctg gttgctaatc cgtggcagct gggcaacgtt
     5161 gccgcgatgc tcgaacgtgc tggcgacgat gctgcgcgtc cggaatggct ggagccagag
     5221 ttcggcgtgc gtgatggtct gtactacctg accgaacagc aagctcaggc gattctggat
     5281 ctgcgtttgc agaaactgac cggccttgag cacgaaaaac tgctcgacga atacaaagag
     5341 ctgctggatc agatcgcgga actgttgcgt attcttggta gcgccgatcg tttgatggaa
     5401 gtgatccgtg aagagctgga gctggttcgt gaacagttcg gtgacaaacg tcgtactgaa
     5461 atcaccgcca acagcgcaga catcaacctg gaagatctga tcactcagga agatgtggta
     5521 gtgacgctct ctcaccaggg ctacgttaag tatcagccgc tttctgaata cgaagcgcag
     5581 cgtcgtggcg ggaaaggtaa atctgccgca cgtattaaag aagaagactt tatcgaccga
     5641 ctgctggtgg cgaacactca cgaccatatt ctgtgcttct ccagccgtgg tcgcgtctat
     5701 tcgatgaaag tttatcagtt gccggaagcc actcgtggcg cgcgcggtcg tccgatcgtc
     5761 aacctgctgc cgctggagca ggacgaacgt atcactgcga tcctgccagt gaccgagttt
     5821 gaagaaggcg tgaaagtctt catggcgacc gctaacggta ccgtgaagaa aactgtcctc
     5881 accgagttca accgtctgcg taccgccggt aaagtggcga tcaaactggt tgacggcgat
     5941 gagctgatcg gcgttgacct gaccagcggc gaagacgaag taatgctgtt ctccgctgaa
     6001 ggtaaagtgg tgcgctttaa agagtcttct gtccgtgcga tgggctgcaa caccaccggt
     6061 gttcgcggta ttcgcttagg tgaaggcgat aaagtcgtct ctctgatcgt gcctcgtggc
     6121 gatggcgcaa tcctcaccgc aacacaaaac ggttacggta aacgtaccgc agtggcggaa
     6181 tacccaacca agtcgcgtgc gacgaaaggg gttatctcca tcaaggttac cgaacgtaac
     6241 ggtttagttg ttggcgcggt acaggtagat gactgcgacc agatcatgat gatcaccgat
     6301 gccggtacgc tggtacgtac tcgcgtttca gaaatcagca tcgtgggccg taacacccag
     6361 ggcgtgatcc tcatccgtac tgcggaagat gaaaacgtag tgggtctgca acgtgttgct
     6421 gaaccggttg acgaggaaga tctcgatacc atcgacggca gtgccgcgga aggggacgat
     6481 gaaatcgctc cggaagtgga cgttgacgac gagccagaag aagaataatt ttacttcttc
     6541 atgccaaaag ggagctatct cccttgtttg aattgaaaag tccaggctgc aaagtctggg
     6601 cttttgtcgt attagggcac ggtaaagttt ggctgtgccc gtaaaaaatg gctggctata
     6661 cacaaggaat gtggcaatga gtggtgaaaa aaaggcgaaa ggctggcggt tctatggtct
     6721 tgtaggtttt ggcgcaatag tactgctttc cgctggcgtc tgggcgttgc aatatgctgg
     6781 cagtgggcca gaaaaaacgt tgtcgccgct ggtgatgcac aacaatctgc aaatcgatct
     6841 caatgagccg gacctctttc tcgacagcga ctctctgagc cagcttccca aagatctcct
     6901 caccattccg tttctccacg atgttctgag tgaagatttc gttttctatt atcagaatca
     6961 tgccgatcgt ctgggcattg aaggcagcat tcgtcgcatt gtctatgaac acgatctcac
     7021 gctgaaagat aaactctttt cgtcactctt agatca
//
LOCUS       DADLFA010000189         7031 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000189 DADLFA010000000
VERSION     DADLFA010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7031
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.191"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            598..882
                     /locus_tag="JQH65_003500"
     CDS             598..882
                     /locus_tag="JQH65_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAY6140791.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            870..1355
                     /locus_tag="JQH65_003501"
     CDS             870..1355
                     /locus_tag="JQH65_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY6140792.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKGLKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
     gene            1389..2567
                     /gene="ltrA"
                     /locus_tag="JQH65_003502"
     CDS             1389..2567
                     /gene="ltrA"
                     /locus_tag="JQH65_003502"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAY6140793.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVKHDLVLKALEHHGMPKWVILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDATRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNF
                     KGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARRYNTIVRGWIEYYGKF
                     WSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE
                     "
     gene            2650..2804
                     /locus_tag="JQH65_003503"
                     /pseudo
     CDS             2650..2804
                     /locus_tag="JQH65_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2858..3340)
                     /locus_tag="JQH65_003504"
                     /pseudo
     CDS             complement(2858..3340)
                     /locus_tag="JQH65_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(3596..4576)
                     /locus_tag="JQH65_003505"
     CDS             complement(3596..4576)
                     /locus_tag="JQH65_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAY6140794.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(4576..5775)
                     /locus_tag="JQH65_003506"
     CDS             complement(4576..5775)
                     /locus_tag="JQH65_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAY6140795.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(6097..6435)
                     /locus_tag="JQH65_003507"
                     /pseudo
     CDS             complement(6097..6435)
                     /locus_tag="JQH65_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(6502..>7031)
                     /locus_tag="JQH65_003508"
     CDS             complement(6502..>7031)
                     /locus_tag="JQH65_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY6140796.1"
                     /translation="APQKQLQSLRSLSFIERNENIVLLGPSGVGKTHLAIAMGYEAVR
                     AGIQVRFTTAADLLLQLSTAQRQGRYKTTLQRGVMAPRLLIIDEIGYLPFSQKEAKLF
                     FQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAMLGRILHHSHVVQIKGESYRL
                     RQKRKAGVIAEANPE"
BASE COUNT         1883 a         1598 c         1679 g         1871 t
ORIGIN      
        1 cggcatccgg catataggca gcatgaacgc aacacaggct ctcgtacgga acgtgggaac
       61 ctgtcgtctc gatgctaagg gagcggcgcg gagcagaggc actgcaagcg cctgagtacc
      121 gatgcgagac acaggggcag atggcttcgt agtagtgagg aagtgcctgt aatgggcatg
      181 gagcgaaggg ggtcagtcga ggcgtttgag gttaccaatc gaagctatac ccggtaatgg
      241 cgcaggttga cctgcacttg gccaagtgga ttgcccgcaa acacaagcga gcgcgaggta
      301 gtctggtgcg tgcattcgag tggttactgc gaattcgtca cgactgcccg acgctgtttg
      361 cacactggtg cttagcttac gatacttgaa tggttcaacg acaagagccg tatgacgcga
      421 gagtgtcacg tacggttccg tgggagcgta ggggggcggt gcccctgcgc cacccgatat
      481 cccctttgag tgcggttacg tccacagagg taaatgccgc tgacgcggca tctgtaatct
      541 gttactttgt gtacatcatt tgctatatat tgtcaatatg ctattgttgt aaagcaaatg
      601 atatacggag gctttatgaa atcaggtgtt cagctcaatc tcagagcaag agaatcacag
      661 cggatactca ttgatgctgc tgcagaaatt cttcataagt cgcgtacaga tttcattctg
      721 gaaatggcct gtaaagctgc tgaagatgtt attctcgacc ggcgggtgtt taattttaat
      781 gacaggcaat atgaagagtt cattgaaatg cttgatgctc cggttgcaga tgatcctgcc
      841 atcgaaaaat tgctggcaag gaaacctcaa tgggatgtgt aactgcacca gaacctttat
      901 cctcatttca tcaggtcgct gaatttgtca gcggtgaagc tgtacttgat gactggttaa
      961 agcagaaggg gctaaaaaat caggcaatcg gagccacccg gacttttgtt gtatgcagaa
     1021 aaggtacgca acaaatagtc ggtttttact cactggccac tggtagtgtc aatcatacag
     1081 aagctacagg taaccttcgg cgcaatatgc cagaccccat tcctgtaatt atacttgcac
     1141 gcctggctgt agatgtgtca tttcgaggaa aagggcttgg cgcggattta cttcatgatg
     1201 cagttcgtcg ttgttacagg gttgccgaaa atatcggtgt gcgtgcaatt atggttcatg
     1261 cgcttacgga aaacgcaaaa cagttttata ttcatcatgg ttttaagcca tcaaaaactc
     1321 aggtgcaaac attgtttctt aaactccctc aatagggagt aatgcactgg tctgggcatc
     1381 atatctggat gtgcgtcgca acaagggagc cccccggatg cgacgggcag actttgaaaa
     1441 tgttcgacca gcaacgtgat ggcaatctat acaagatctg gaaccggcta tgttcgggaa
     1501 catggtttcc gccaccagtg ctggaaaaac ggatcccgaa gtcgaatggc aaggaacgca
     1561 ttctggggat cccaacagta tccgatcgaa ttgctcaggg agcgataaaa cttttcatgg
     1621 aagaaaagct tgacccgatt ttccatgctg attcatatgg ttaccgacca ggcaaatcag
     1681 ctcatgacgc tctgaaacaa tgtgccatcc ggtgctggcg ttatagctgg atactggaag
     1741 tggacatcag tgcgtttttc gaccatgtga aacatgatct ggtgctcaaa gctctggaac
     1801 accacgggat gcctaaatgg gtcatcctgt actgtcgacg ctggatggaa gcgccaatgc
     1861 agagttgtga aaatggagaa ttaataaccc gaacacgagg cactccgcag ggcggggtca
     1921 ttagtccgct acttgctaac ttgttccttc actatgcatt tgatttgtgg atggaaagag
     1981 aatatcgggg ggtaccgttt gagaggtacg ctgacgatat tgtagtacat tgttcacgaa
     2041 tgagtgatgc aacaaggcta aagaacaggt tatcggagcg cttctcggaa gtcgggctgg
     2101 tcctgaatgc agggaaaacg aatatcgcct acattgacac gtttaaaagg agaaacgtcg
     2161 caacgagttt cacctttctc ggatatgact tcaaagtgcg tacgctgaag aatttcaaag
     2221 gcgaacggta ccgaaaatgc atgccgggtg cgtcaaatgc agcaatgcgc aaaataacag
     2281 aaacaatcaa gaagtggcgt atacatcgct caacagctga gagtttgctg gattttgcga
     2341 ggcgctacaa tacgatagtg agaggctgga tcgagtacta cggaaagttc tggtccagaa
     2401 atttcaacta tcgactgtgg agtgcaatgc agtcacgtct gctcaagtgg atgcagtcta
     2461 aatacagact ttcgaaccgg aaggctcagc gaaagctgac gctggtaagg aaggagtatc
     2521 cgaagctatt tgttcactgg tatttactac gtgcatcaaa tgagtgatca agagccgtat
     2581 gacgcgagag tgtcacgtac ggttccgtga gcagccgggg agtgaaattc tctccggctg
     2641 actcgactcg cgggttccga cagcgatgac gaacatgcgg cggtgttgta ctcgctgatc
     2701 ggcacatgcc gtctgaacaa tgtggaacca gaaaatggct gcgttacgtc attgagcata
     2761 tccaggactg gccggcaaac cgggtacgtg atctgttgcc ctagaaagtt gatctgagct
     2821 ctcagtaaat atcaatacgg ttctgacgag ccgcttactt ttggcgtaat tctgctgcgc
     2881 ctttactgaa cacaatgccg tattcagcaa gaagtccacg aatgtgattt atcagttgtg
     2941 tcctttccat tgaaaccaac cgacgcatat tcaaaaaagt ttggacgtcc tgttgctcca
     3001 gagtttttat tggtacgggt cgtatagttc catgttggca ggcctcagct attgcctgtg
     3061 cgtcgttata atcatttttc tggcaccgct gataagcttt aacatgttgt gccggaagca
     3121 atattacatc atggtcgagt ttcctgattt cccgggccca gtaatgagat gccccgccag
     3181 cctccatcgc aacagtcatt ttaggctgtt gcaccagcca gttaagcagt tgattgcgag
     3241 aaagtttggt ctttcctgcg ggtttgccat gagcattgat actgaaaaga taaaagttcg
     3301 ttttagccag atcaatgccg gtgattttct gttcagacat ggtatcccgc ttacttttgt
     3361 ggttaaagtt tatccatcat ggcgcaattg aaggctgccg tgaaagaggg ggaagactat
     3421 ctcatcggtt actgttaaac ggctaagaga aactatcttt ttcaagcaaa gaagatacgg
     3481 tgaaacaggc gatttcaccg tgaaatactg ttcaaaaaaa acgctaataa aagcaaaatt
     3541 tcttttaaaa cagccaatta aaaactggtg agttgctggc gaaaaacttg aattttcaca
     3601 gcgaattcag attatctttt agtacaagag caatcatacg atctaactta tcctgaactt
     3661 ccaacggtaa tctgccgaat tcatatgaga acgtacgtcc ctttactcgt tttcgagcaa
     3721 atttatcttt actatcaaat ttccataaca aagttacaac cgctttatcc tttactcctg
     3781 aatcaagcaa tgaagacatt tccttggtta taagccgcat aatactgttt ttaacctcgt
     3841 cttcagaaag attgtcatca gcagtaataa gctcaatact cggcgagata ttcttcacaa
     3901 cttcatcaat agatatatca cccagcctca gtcgttcact taattcagcc agttgcctgt
     3961 aatcggcgta tgtcagctca gactgaacag gaaaaagaga cacaaaatct ttaggtacgg
     4021 acgctgcttg gagggcacga gtcaccttag ctgccgacaa tccctctctt tccgcaattt
     4081 gcttctggtt cataccggcc tctttgagac gtactaaacg caacccaatt tcacgaatat
     4141 tatgttcaag tgacgtctgc aaatctttcg ccagatgctg agcttcagaa acagtaagct
     4201 catcatccgt tacaagtaca cgtaatccaa ccttgcatag caaagctgct gcccgacgac
     4261 gggaaccatc caaaatctca ataagatcgc cagtccgaat cccaatacat ggatagaatt
     4321 gttgtaaacg tatagttctg gtgatgtctt ttagtgagtc cggcgtcagg gctgtctgtt
     4381 cgcggccatt cacctcttca accacaaaag tttgagtgtc cactttgtca tgatcaacct
     4441 tgatctcagt aaattttgcc tttcggccag tattgagggt aaatacatga actggagccg
     4501 atatttcttc agtattattc aggatatttg tattcagcgt acggccaata gtcggtcggt
     4561 gctttctgtt ttccatcagt agttggccct tataaattca atacgatcaa aaactgcttt
     4621 agcgaaatct tctgctgcca tacgtgcatt tttcaaagct tcaccactgc caacataagt
     4681 aacaggattc gcagatatca cggtatcaaa cgactcgccg cttctctcaa aaccatcaag
     4741 acgtggcatt gaaacatcaa gcatgtcacc accgaaaatc tcttttgtca ggctatggca
     4801 gtatttatga tctgacttat tcgccagttt cgacatgaag ccaatatttg cctgcaaacg
     4861 gcatgaacat ccatcctgtt caataatctg tacaagttca ggtaaacgag ccaaatattt
     4921 aagcgtggaa tggaaatcaa cttgtgctgg cggcaccggt gtaaacatga tatctgcggc
     4981 agcaatggca tttttcaaaa atgcgtccag atgcgggcct gtatcaataa ggatgaagtc
     5041 gaaatcatgc ttcagcttat caataatatt ctcacgcaat atcgcgtgtt tgttttgtcc
     5101 taatagatgc tcctcgcaca gagtatccca gtttgatgca ataaaggcgt cgtcaataga
     5161 agccggcata acataaacgc cagggataac tgacggaacg ataaaatctt ccagtaattc
     5221 ctcacgactc acgttctgca acatagcctg cggtgcggtt gtatcaacca gccccaccgc
     5281 atgcaagtag ttcagaaaca tagtggctga tgactgggga tctaaatcaa ggagcagaat
     5341 acgtaaatct tctgacagca ggtgaggatg agcacgcagg gcgtgagcta cggaaacact
     5401 actcacggtt ttagacacac cgccttttag actaccgatg aatatggaat atgcctcact
     5461 ataacgatcg cgatatttag gaatgccacg atgtgcatag atatcgataa tgttctggat
     5521 ggttaatgca tattttgtcg cggttcctgc tgggcgtttt tcaaactgat agccctgagc
     5581 ctccatctca ccgactgcac catccacgct tgcgcgcgtt aattttggaa gcttagctac
     5641 agcagacttt gaatagactt gataaaaaac atcagcctga aactcctgct tttgctcctg
     5701 aatctgtttt gttagggcga gcaacatttt atctgcacgc tgcgcaacct tacttaactg
     5761 ctcaaaacta gtcattcccc ctccgtgcta tgtaggattt taacttatta caaaaaaaac
     5821 tgcattagca gggtaatgca tgcaagtgca tattgtacat gcttatatta aaaatgcgcg
     5881 cattgctgat ttattaatca tcggaatgaa tattactgta agatagtatg cctttccggg
     5941 agaaagacgc tgatttcccc cgaataatcc agttcaccac cacactccgg acaacagctt
     6001 tcttcaggct ccagatggtg tatttcgcgg gaaacatgtt ccggtaacgg acggcgatga
     6061 cgtgactgtc gcaacaggcg ggggtagtgc ttccaatcag tagaacatgt gcttttcaat
     6121 gaaggagccg atgacttttt cgtggatttc cacggaacgc gagaagcaga ttgttttacg
     6181 tgccagccgc ttgatgcggg tgcggagggt ccggttgtta cgttcaatac gttgcgtgaa
     6241 gattttgccg gtcaggtgtt tctccttcgg cacttctctg gcgtagctgc cccagtcatc
     6301 gctggtcacc atgccgatac agaacggagt caacagggct aacagttccc gacaggtctc
     6361 atcattccgt ggcccgaaag tgtaagccaa cacaccgcca gttttagtgt tgtacgcata
     6421 ccagagccag tgttgtgtca acgacggatg aaaagtgatc cacttatatc tccaccaacg
     6481 gcccaatatt gatccaccgt tttactcagg attagcttct gctataaccc cggcctttcg
     6541 tttctgtctg agtcgatagc tttctccttt gatttgaacg acatgtgagt ggtgtaagat
     6601 acggcccagc atcgctgagg tcagtgctgc atcaccggcg aacgtttgat cccactgccc
     6661 gaacggtaga ttggatgtca ggatcattgc gctcttttca taacgtttag cgatgacctg
     6721 gaagaacagt tttgcttctt tctgactgaa cggcagatag cctatttcat caatgatgag
     6781 caggcggggg gccattactc cacgctgaag cgtcgtttta taacggccct gacgttgtgc
     6841 cgtagataac tgaagtaaca gatctgctgc tgttgtgaag cgaacttgga tacctgcacg
     6901 gactgcttca tagcccatcg cgattgccag atgggttttc cccacacctg atggccccag
     6961 taatacgata ttttcattac gttctatgaa gctgagtgag cgtaacgact ggagttgctt
     7021 ctgcggtgct c
//
LOCUS       DADLFA010000190         6974 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000190 DADLFA010000000
VERSION     DADLFA010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6974
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.192"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(423..908)
                     /gene="sixA"
                     /locus_tag="JQH65_003509"
     CDS             complement(423..908)
                     /gene="sixA"
                     /locus_tag="JQH65_003509"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HAY6140797.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(1111..3255)
                     /gene="fadJ"
                     /locus_tag="JQH65_003510"
     CDS             complement(1111..3255)
                     /gene="fadJ"
                     /locus_tag="JQH65_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HAY6140798.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTVQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTTLEMILTGKQLRAKQALKLGLVDDVVPHSILLEVAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTKGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVSERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3255..4565)
                     /gene="fadI"
                     /locus_tag="JQH65_003511"
     CDS             complement(3255..4565)
                     /gene="fadI"
                     /locus_tag="JQH65_003511"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HAY6140799.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLFGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFACEVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGAWMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4745..5029)
                     /locus_tag="JQH65_003512"
     CDS             complement(4745..5029)
                     /locus_tag="JQH65_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 family protein"
                     /protein_id="HAY6140800.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5401..6741
                     /gene="fadL"
                     /locus_tag="JQH65_003513"
     CDS             5401..6741
                     /gene="fadL"
                     /locus_tag="JQH65_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HAY6140801.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGNTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1784 a         1846 c         1628 g         1716 t
ORIGIN      
        1 cttaaaaaat acggaataag tatttagaaa aaatagaaag tatatcaata atgaatatca
       61 acagcttaaa aaacaaacaa tgaacatata atgcgattgg catttatcca cttttgacaa
      121 tataatatat aaacactata gccagaattt aatcaagttt caattacatc ctgcctgatt
      181 attcaactct attatctgta ttaataaatg acaatgagtg tcaaacagac attattcatt
      241 tcaacatgta actttgggca aaatcaagat acgtgcagaa gaataatttc cgaatgaatg
      301 tagaaagtgt tttatttata gataaggaga gtcttcgata gtagtgaggt tgacagatat
      361 ccgctgcctg atgagcaatt atgcccaatg aatctacctc attgggcata atttggaaca
      421 catcagatag cttttgccat cttcagatta cacgggctca tctgccagtt gaatgtaccg
      481 tttccgcttt catcaagcgt tacgctggca atggccgagg tggtaaacat aggcggcgtt
      541 tcgcccggac ataactcggc aaccagatac ccgactaacg gtaagtgaga gatcaccagt
      601 accgaagcaa caccttcatt ggtcaacgcc tgcaaatagg cactgaccag accaacatcg
      661 ccgcagggcg ttaactccgg cagaacttct gcactggaag gcaggttcag acaatcccct
      721 acttcttcca gtgtttgctc ggctcgcagg aacgggctca ccagaacgcg ttcgatttcc
      781 actttttgac ctttcagcca gttcgccatc aggcgagatt cgtcacaacc attagtggtc
      841 agaggacgaa ccgaatcact ggcggcatcg agggctgcgt cgccgtgacg catgataaaa
      901 acttgcatat tgcaccgctt ttgttaacca gattcgccca ttttctttca tctgaaacca
      961 gagaaagaaa acggtggccg gcattgtgcc ttatccattc accgaatgaa acgctgtttt
     1021 ttacctcaat ggcgtaagta tagtcaatat gcgtttacat tttgaccaat actccgccat
     1081 tcagcgcgga ttcatatagc tttgaccttc ttattgcagg tcagttgcag ttgttttcca
     1141 aaaactttcc ccacgcgcgc ccatctcgac caaacgctcg caaggggtaa aacgggaacc
     1201 atactgcgtg gccagtcgtt gcattattgc aaccacttcg cccgcgccga gagaatcgat
     1261 atagcggaac ggtccaccga gaaatggcgg aaaaccaatg ccaaataccg caccaatatc
     1321 cccgtcacgc acgctacgga taacctgctc atcaacacaa cgtactgctt cattcagcat
     1381 caacatcaca caccgttcag aaacctgcgg tgcggagagt cgcccctgcc cttgtgcgcc
     1441 aatgagcggg taaatggcgg gatcgacctg ttttttgctt ttacgccctt tctgaccata
     1501 aagatagaaa ccccgaccat tttttctgcc tttgcgatcg tcgttcaaaa ttgaagaaac
     1561 aacatttgca ggcgcgctaa aacgttctcc ataagcggct tccagtacag gaataatttt
     1621 agtcccggtg tcgattccta cctcatccaa aagttggatt ggacctaccg gaaaaccaaa
     1681 tttcactagc gcggcatcaa tgtgctctac ccgttcacct ttggtcagca tgcggatagc
     1741 ttcattaatg taaggcgcta agatgcgatt gacgtaaaac ccggctttgt cacgcacgac
     1801 aatgggcgtt ttgccctgtt ttttcgccag ttttaccgta gtggcgatgg tttgcgctga
     1861 tgtacccgca tgaggaataa tctccaccag cggcattttt tccaccggac tgaagaaatg
     1921 caggccgata acttgctcag gtcgcgtggc gtgagcggcg atatcaccaa tcggtaaaga
     1981 tgacgtattc gaagcaaaga tggtatgagc ggcgcaattt tgctcaactt ccgccaccat
     2041 ctgttgtttc aattcgagat tttcaaacac cgcttcaata atcagatcgc gatgggcaaa
     2101 gccgcgatag tccgtcgttc cggagattaa tgccagctgt ttgtcacgtt cgctggcttt
     2161 gagatgacga cggcgaactt tgccctccag ctgatcccaa ctgtacttca gcgcatgatt
     2221 tatgccctgc gggttgatat ctttaattct gaccggaagc cccgctttac aagcagtgac
     2281 ataagcaata ccgccgccca tcaagccacc acctaaaatt cccacgctgt ttaatggcgc
     2341 aggcggtgca tcactaccgg gatctttctt cacgtccgta ctggcaaaaa agatactacg
     2401 cagcgcctgc gattgtggcg tcatcgccag ttcgccaaac gcccgagctt cggcgtcata
     2461 accgctgctg gtgccctgcg ctaatcccgt ttcaacaacc tccaggatgc gttctgtcgc
     2521 cggataattg ccttgagttt tgtgttctgt tttcttgccg accattttga acagcagcgc
     2581 acgacctaac ggccccgcca gaatacgctc gcgtacaggt agagggcggg aagatgggcg
     2641 atccttcttc gccagctcaa cagcgacttc cagcaaaatg gagtgcggaa caacgtcatc
     2701 caccagcccc agctttaatg cctgtttcgc ccgaagttgt tttccggtga ggatcatctc
     2761 taatgttgtg ctgacgccta tcagacgcgg taaacgctgg gtgccgcctg aaccgggtaa
     2821 caatccaagt tgtacttcag gcaaaccgag caccgtttta ggatcgtcag tacaaacgcg
     2881 accgtggcac gccagcgcca actccagccc accacccagg caagcgccat gaatagccgc
     2941 gataaccgga atgggcaaag catgaatctc cgccatcaac tgttggccct gccgcgccag
     3001 agcttccgct tcttgcaccg ttttgcagtt gccgatcatg ttgatgtctg ctccggcgat
     3061 aaaattgtcc ggtttcgcgg agataaacac cacgccgcgc aactctttgt tttcacggag
     3121 ttgcttaata atggcgcgca cctgcgaggc aaattccgcc ttcagggtat tcattttctc
     3181 atccggtacg tcgatggtga tgatagcaat gttgtccaga cgaacattaa gagtaaacgc
     3241 tgatgccatt tccattattc cgcctccaga accattgccg cgccaagccc accggcagca
     3301 caggcggtaa ctaaaccaaa tccaccgccg cgacggcgaa gttcatgcaa tgtctgggta
     3361 atcatccacg cgccggtcgc cgcgaaggga tgcccgtaag caatcgaacc gccaagcacg
     3421 ttaaatttgc tatcgtccac ttcgccagtg gcatgtgcac gccccagtac ttcacaagca
     3481 aaacgttcac tacccagcaa ctgaatattc gccagtgtct gagctgcaaa ggcttcgtgc
     3541 atatcgatca atgtcagatc gctcattgtc aaaccggcac gttccagcgc cagcggtgtt
     3601 gaccaggccg gaccgaacaa catgtcctgc cagacatcaa tcgcagtaaa tgcgtagctg
     3661 cgcagatacc ccagcggcac cagccctaat tctttcgccc gggattcagt catcaggatc
     3721 accgctgccg cgccatcggt cagcggcgta ctgtttgccg ccgttaccgt tccgtgtttg
     3781 cgatcaaacg ccgggcgcag ctttgcgtaa tcggcaagcg aggaattacc gcgaatattg
     3841 ttgtcttcga caagcggttg tttataagga gggataaagg cagtcatcac ctcttctttg
     3901 agttttccgt ctgaccatgc ctgagcggca cgctgatgcg aacggtgcgc taatgcatct
     3961 tgctgttctc gggtgatgcc gtaggttttc gccatttgct ctgcggtgtc acccatccgc
     4021 aagccggtag aatattctgc tactgcaggc ggtacgggca ttaaatcgcg caaacgcagg
     4081 cgagagaaga gtttcaggcg ctggctcatg gttcgagctt tgttgacatc aaccagcacg
     4141 cgcgccagtt ttttactgac gccaattggc aatacggaag aggaatctgc cccaccggca
     4201 atccccgctc ggatagttcc cgccatcagg ctttctgcga cgtttgcaac cgcctggaaa
     4261 ctggtagcgc aagcgcggct gacgctgtaa gcatcggtat gcacattcat tccggtaccg
     4321 agaacaattt cacgcgcaat gttgggggct tcaggcattt gtacgacctg accaaagacc
     4381 agctgttcaa tcacttcagc ggggatctcg ctgcgtgcca gcagttcgcc taccaccatc
     4441 ttccctaaat caaccgcggg aatgccatga aaagccgtcg cctggcgggc aaaaggcgta
     4501 cgtaaaccgc taacaatggc gatacgatcg ccctggcggg taaccagcgg taaaacctga
     4561 cccataacac tcccctgtaa aaaaataaat aaagtggtct gacctgatta tagtcttaac
     4621 cattttttta catttagcca agcggagaaa aaggaaagtg ggagctatga cacagtgaag
     4681 acaggagaaa gaaaagaaaa cgcccctgcc agcaactgac aggggcgaac gtacccgaga
     4741 ggaattaacg cagacccagc tggaagatca gcatttcagc ttcgcaggca aaagtgaaat
     4801 cgatatccag gcgcacaccg tcagactctt cagtgaaagt cggggagatt ttgcacggtt
     4861 cggattccac gctacgggct ttttcagtca acgccgccag cgtttgttct gcttctgcgc
     4921 ggtttgcaaa cacgcggctg taagacgcgg tgcagtcgga gttgtccata atggtgccaa
     4981 catccataca gcagcaaacc ggggtttcat cagcactaca tttactcatc gttgatttcc
     5041 tctgtatgtg cacccaaggt gccagataaa cgttgtggat attttacgct tccggaaagt
     5101 gctgctccag ttgttaattc tgcaaaatcg gataagtgac cgaaatcaca cttaaaaatg
     5161 atctaaaaca aaattcaccc gaatccgcga gtgcgcaacc tccaaatttt gccagctgga
     5221 tcgcgtttct tagatcatat ttgaaaaaag atggaaacat acttgcaaca ttccagctgg
     5281 tccgacctat actctcgcca ctggtctgat ttctaagatg tacctcagac cctacacttc
     5341 gcgctcctgt tacagcacgt aacatagttt gtataaaaat aaatcattga ggttatggtc
     5401 atgagccaga aaaccctgtt tacaaagtct gctctcgcag tcgcagtggc acttatctcc
     5461 acccaggcct ggtcggcagg ctttcagtta aacgaatttt cttcctctgg cctgggccgg
     5521 gcttattcag gggaaggcgc aattgccgat gatgcaggta acgttagccg taaccccgca
     5581 ttgattacca tgtttgaccg cccgacattt tctgcgggtg cggtttatat tgacccggat
     5641 gtgaatatca gcggaacgtc tccgtctggt cgtagcctga aagccgataa catcgcgcct
     5701 acggcatggg ttccgaacat gcactttgtt gcaccgatta acgaccaatt tggttggggc
     5761 gcttctatta cctctaacta tggtctggca acagagttta atgatactta tgcaggcggc
     5821 tctgtcgggg gtacaactga ccttgaaacc atgaacctga acttaagcgg tgcgtatcgc
     5881 ttaaataatg catggagctt tggtcttggt ttcaacgccg tctacgctcg cgcgaaaatt
     5941 gaacgtttcg caggcgatct ggggcagttg gttgctggtc agattatgca atctcctgcc
     6001 gggcaaactc ctcaagggca agcattggca gctaccgcca acggtatcga cagtaatacc
     6061 aaaatcgctc atctgaacgg taaccagtgg ggctttggct ggaacgccgg aatcctgtat
     6121 gaactggata aaaataaccg ctatgcactg acctaccgtt ctgaagtgaa aattgacttc
     6181 aaaggtaact acagcagcga tcttaatcgt gcgtttaata actacggttt gccaattcct
     6241 accgcgacag gtggcgcaac gcaatcgggt tatctgacgc tgaacctgcc tgaaatgtgg
     6301 gaagtgtcag gttataaccg tgttgatcca cagtgggcga ttcactatag cctggcttac
     6361 accagctgga gtcagttcca gcagctgaaa gcgacctcaa ccagtggcaa cacgctgttc
     6421 cagaaacatg aaggctttaa agatgcttac cgcatcgcgt tgggtaccac ttattactac
     6481 gatgataact ggaccttccg taccggtatc gcctttgatg acagcccagt tccggctcag
     6541 aatcgttcta tctccattcc ggaccaggac cgtttctggc tgagtgcagg tacgacttac
     6601 gcatttaata aagatgcttc agtcgacgtt ggtgtttctt atatgcacgg tcagagcgtg
     6661 aaaattaacg aaggcccata ccagttcgag tctgaaggta aagcctggct gttcggtact
     6721 aactttaact acgcgttctg ataacgcgtt cgcctggata aagtcacctg catagcaggt
     6781 gactttaact cccccacttc accgaaagta gtgtcctcat tgcttacctc ctgagttttg
     6841 caaacagcct gttggcagct tgccctttca aatcaataag cggtatccac agacaaacct
     6901 gaagggaaag gcatattttc aggcgttctg ctcgtccttc tcaaagagtt tacttttctg
     6961 catttccagg atac
//
LOCUS       DADLFA010000191         6800 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000191 DADLFA010000000
VERSION     DADLFA010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6800
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.193"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            186..2102
                     /gene="dnaK"
                     /locus_tag="JQH65_003514"
     CDS             186..2102
                     /gene="dnaK"
                     /locus_tag="JQH65_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAY6140802.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            2191..3321
                     /gene="dnaJ"
                     /locus_tag="JQH65_003515"
     CDS             2191..3321
                     /gene="dnaJ"
                     /locus_tag="JQH65_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAY6140803.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGQHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(3425..3634)
                     /gene="mokC"
                     /locus_tag="JQH65_003516"
     CDS             complement(3425..3634)
                     /gene="mokC"
                     /locus_tag="JQH65_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HAY6140804.1"
                     /translation="MLNTCRVPLTDRKVKEKRAMKQHKAMIVALIVICITAVVAALVT
                     RKDLCEVHIRTGQTEVAVFTAYESE"
     gene            4163..5329
                     /gene="nhaA"
                     /locus_tag="JQH65_003517"
     CDS             4163..5329
                     /gene="nhaA"
                     /locus_tag="JQH65_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HAY6140805.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            5395..6294
                     /gene="nhaR"
                     /locus_tag="JQH65_003518"
     CDS             5395..6294
                     /gene="nhaR"
                     /locus_tag="JQH65_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HAY6140806.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(6332..>6800)
                     /locus_tag="JQH65_003519"
     CDS             complement(6332..>6800)
                     /locus_tag="JQH65_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6140807.1"
                     /translation="RFNYNLNTILLVNLMTCGFEDQSIDTGRFVLSRINNENYQFHHI
                     RFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKNELENSEDGAGQVGFQLSNDG
                     TNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
BASE COUNT         1738 a         1601 c         1757 g         1704 t
ORIGIN      
        1 ctgcgcaaaa gcacaaaaaa tttttgcatc tcccccttga tgacgtgggt tacgacccca
       61 tttagtagtc aaccgcagtg agtgagtctg caaaaaaatg aaattgggca gttgaaacca
      121 gacatttcgc ccctattaca gactcacaac cacatgatga ccgaatatat agtggagacg
      181 tttagatggg taaaataatt ggtatcgacc tgggtactac caactcttgt gtagcgatta
      241 tggatggcac cactcctcgc gtgctggaga acgccgaagg cgatcgcacc acgccttcta
      301 ttattgccta tacccaggat ggtgaaactc tggttggtca gccggctaaa cgtcaggcag
      361 tgacgaaccc gcaaaacacc ctgtttgcga ttaaacgcct gattggccgc cgcttccagg
      421 acgaagaagt acagcgtgat gtttccatca tgccgttcaa aattattgct gctgataacg
      481 gcgacgcatg ggtcgaagtt aaaggccaga aaatggcacc gccgcagatt tctgctgaag
      541 tgctgaaaaa aatgaagaaa accgctgaag attacttggg tgaaccggta actgaagctg
      601 ttatcaccgt accggcatac tttaacgatg ctcagcgtca ggcaaccaaa gacgcaggcc
      661 gtatcgctgg tctggaagta aaacgtatca tcaacgaacc gaccgcagct gcgctggctt
      721 acggtctgga caaaggtact ggcaaccgta ctatcgcggt ttatgacctg gggggtggta
      781 ctttcgatat ttctattatc gaaatcgacg aagttgatgg cgaaaaaacc ttcgaagttc
      841 tggctaccaa cggtgatacc cacctgggtg gtgaagactt cgacagccgt ctgatcaact
      901 acctggttga agaattcaag aaagatcagg gcattgacct gcgcaacgat ccgctggcaa
      961 tgcagcgcct gaaagaagcg gcagaaaaag cgaaaatcga actgtcttcc gctcagcaga
     1021 ccgacgttaa cctgccgtac atcactgcag atgcgaccgg tccgaaacac atgaacatca
     1081 aagtgactcg tgcgaaactg gaaagcttgg ttgaagatct ggtaaaccgt tccattgagc
     1141 cgctgaaagt tgcactgcag gacgctggcc tgtccgtatc tgatatcgac gacgttatcc
     1201 tcgttggtgg tcagactcgt atgccaatgg ttcagaagaa agttgctgag ttctttggta
     1261 aagagccgcg taaagacgtt aacccggacg aagctgtagc aatcggtgct gctgttcagg
     1321 gtggtgttct gactggtgac gtgaaagacg tactgctgct ggacgttacc ccgctgtctc
     1381 tgggtatcga aaccatgggc ggtgtgatga cgacgctgat cgcgaaaaac accactatcc
     1441 cgaccaagca cagccaggtg ttctctaccg ctgaagacaa ccagtctgcg gtaaccatcc
     1501 atgtgctgca gggtgaacgt aaacgtgcgg ctgataacaa atctctgggt cagttcaacc
     1561 tggatggtat caacccggca ccgcgcggca tgccgcagat cgaagttacc ttcgatatcg
     1621 atgctgacgg tatcctgcac gtttccgcga aagataaaaa cagcggtaaa gagcagaaga
     1681 tcaccatcaa ggcttcttct ggtctgaacg aagatgaaat ccagaaaatg gtacgcgacg
     1741 cagaagctaa cgccgaagct gaccgtaagt ttgaagagct ggtacagact cgcaaccagg
     1801 gcgaccatct gctgcacagc acccgtaagc aggttgaaga agcaggcgac aaactgccgg
     1861 ctgacgacaa aactgctatc gagtctgcac tgactgcact ggaaactgct ctgaaaggtg
     1921 aagacaaagc cgctatcgaa gcgaaaatgc aggagctggc acaggtttcc cagaaactga
     1981 tggaaatcgc ccagcagcaa catgcccagc agcagactgc cggtgctgat gcttctgcaa
     2041 acaacgcgaa agatgacgat gttgtcgacg ctgaatttga agaagtcaaa gacaaaaaat
     2101 aatcgcccta taaacgggta attatactga cacgggcgaa ggggaatttc ctctccgccc
     2161 gtgcattcat ctaggggcaa tttaaaaaag atggctaagc aagattatta cgagatttta
     2221 ggcgtttcca aaacagcgga agagcgtgaa atcaaaaagg cctacaaacg cctggccatg
     2281 aaataccacc cggaccgtaa ccagggtgac aaagaggccg aggcgaaatt taaagagatc
     2341 aaggaagctt atgaagttct gaccgactcg caaaaacgtg cggcatacga tcagtatggt
     2401 catgctgcgt ttgagcaagg cggcatgggc ggcggcggtt ttggcggcgg cgcagacttc
     2461 agcgatattt ttggtgacgt tttcggcgat atttttggcg gcggacgtgg tcgtcaacgt
     2521 gcggcgcgcg gtgctgattt acgctataac atggagctca ccctcgaaga agctgtacgt
     2581 ggcgtgacca aagagatccg cattccgact ctggaagagt gtgacgtttg ccacggtagc
     2641 ggtgcaaaac caggtacaca gccgcagacc tgtccgacct gtcatggttc tggtcaggtg
     2701 cagatgcgtc agggtttctt tgccgtgcag cagacctgtc cacactgtca gggccgcggt
     2761 acgctgatca aagatccgtg caacaaatgt catggtcatg gtcgtgttga gcgcagcaaa
     2821 acgctgtccg ttaaaatccc ggcaggggtg gacactggag accgcatccg tcttgcgggc
     2881 gaaggtgaag cgggccagca tggcgcaccg gcaggcgatc tgtacgttca ggttcaggtt
     2941 aaacagcacc cgattttcga gcgtgaaggc aacaacctgt attgcgaagt cccgatcaac
     3001 ttcgctatgg cggcgctggg tggcgaaatc gaagtaccga cccttgatgg tcgcgtcaaa
     3061 ctgaaagtgc ctggcgaaac ccagaccggt aagcttttcc gtatgcgcgg taaaggcgtc
     3121 aagtctgtcc gcggtggcgc acagggtgat ttgctgtgcc gcgttgtcgt cgaaacaccg
     3181 gtaggcctga acgagaagca gaaacagctg ctgcaagagc tgcaagaaag cttcggtggc
     3241 ccaaccggcg agcacaacag cccgcgctca aagagcttct ttgatggtgt gaagaagttt
     3301 tttgacgacc tgacccgcta acctccccaa aagcctgccc gtgggcaggc ctgggtaaaa
     3361 atagggtgcg ttgaagatat gcgagcacct gtaaagtggc ggggatcact ccccgccgtt
     3421 gctcttactc ggattcgtaa gccgtgaaaa cagcaacctc cgtctggcca gttcggatgt
     3481 gaacctcaca gaggtctttt ctcgttacca gcgccgccac tacggcggtg atacagatga
     3541 cgatcagggc gacaatcatc gccttatgct gcttcattgc tctcttctcc ttgaccttac
     3601 ggtcagtaag aggcactcta catgtgttca gcatataggg ggcctcgggt tgatggtaaa
     3661 atatcactcg gggcttttct ctatctgccg ttcagctaat gcctgagaca gacagcctca
     3721 agcacccgcc gctattatat cgctctcttt aacccatttt gttttatcga ttctaatcct
     3781 gaagacgcct cgcatttttg tggcgtaatt ttttaatgat ttaattattt aactttaatt
     3841 tatctcttta tcgcaattat tgacgacaag ctggattatt tttgaaatat tgtcctaaca
     3901 aataacgccg actgacaaga aattaattat tacttttcct aattaatccc tcagaaatcc
     3961 tcaccttaag ctatgattat ctgagcttag ggtcactcgt cagcgcttac agccgtcaaa
     4021 aactcatctc accgctgatg gcgaaaattc tacaaaggct cgtaaaaaac gaattattcc
     4081 tacactataa tctgatttta acgatgattc gtgcggggta aaatagtaaa aacgatttat
     4141 tcacctgaaa gagaaataaa aagtgaaaca tctgcatcga ttctttagca gtgatgcctc
     4201 gggaggcatt attcttatca ttgccgctat cctggcgatg atgatggcca acagcggcgc
     4261 aaccagtgga tggtatcacg actttctgga gacgccggtt cagctccggg ttggttcact
     4321 cgaaatcaac aaaaacatgc tgttatggat aaatgacgcg ctgatggcgg tatttttcct
     4381 gttagtcggt ctggaagtta aacgtgaact gatgcaagga tcgctagcca gcttacgcca
     4441 ggccgcattt ccagttatcg ccgctattgg tgggatgatt gtgccggcat tactctatct
     4501 ggcctttaac tatgccgatc cgattacccg cgaagggtgg gcgatcccgg cggctactga
     4561 cattgccttt gcacttggag tgctggcgct gttgggaagt cgtgttccgt tagcactgaa
     4621 gatctttttg atggctctgg ctattatcga cgatcttggg gccatcatta tcatcgcatt
     4681 gttctacact aatgacttat cgatggcctc tcttggcgtc gcggctgtag caattgcggt
     4741 actcgcggta ttgaatctgt gtggtgtacg ccgcacgggc gtctatattc tggttggcgt
     4801 agtgttgtgg actgcggtgt tgaaatcggg ggttcacgca accttggcgg gggtaattgt
     4861 cggcttcttt attcctttga aagagaagca tgggcgttct ccagcgaagc gactggagca
     4921 tgtgttacac ccgtgggtgg cgtatctgat tttgccgctg tttgcatttg ctaatgctgg
     4981 cgtttcactg caaggcgtca cgctggatgg cttgacctcc attctgccat tggggatcat
     5041 cgctggcttg ctgattggca aaccgctggg gattagtctg ttctgctggt tggcgctgcg
     5101 tttgaaactg gcgcatctgc ctgagggaac gacttatcag caaattatgg cggtggggat
     5161 cctgtgcggt atcggtttta ctatgtctat ctttattgcc agcctggcct ttggtagcgt
     5221 agatccagaa ctgattaact gggcgaaact cggtatcctg gtcggttcta tctcttcggc
     5281 ggtaattgga tacagctggt tacgcgttcg tttgcgtcca tcagtgtgac aggacggttt
     5341 accggggagc cataaacggc tcccttttca ttgttatcag ggagagaaat gagcatgtct
     5401 catatcaatt acaaccactt gtattacttc tggcatgtct ataaagaagg ttccgtggtt
     5461 ggcgcagcgg aggcgcttta tttaactcca caaaccatta ccggacagat ccgggctctg
     5521 gaagagcgcc tgcaaggcaa attatttaag cgcaagggac gtggtctcga acccagcgag
     5581 ctgggagaac tggtctatcg ctatgccgat aaaatgttca ccttaagcca ggaaatgctg
     5641 gatattgtga actatcgcaa agaatccaat ttattgtttg acgttggcgt ggctgatgca
     5701 ctttccaaac gcctggtcag tagcgtgctg aatgctgcag tggtagaagg cgagccgatt
     5761 catcttcgct gcttcgaatc cacccacgaa atgctactgg agcaattaag ccagcataaa
     5821 ctggatatga tcatttccga ctgtccgata gactctacgc agcaggaagg cctgttctcc
     5881 gtgagaattg gcgaatgtgg cgtgagtttc tggtgtacaa atccaccacc agaaaaaccg
     5941 ttcccggctt gtctggaaga acggcgactt ttgattcctg ggcgacgttc aatgttaggg
     6001 cgcaaattgc ttaactggtt taactcccag ggattaaacg tagaaatcct cggcgagttt
     6061 gatgatgccg cgttgatgaa agcttttggt gcaatgcaca atgcaatctt cgttgcccca
     6121 acgctttatg catatgactt ttatgccgat aaaactgtcg tagaaattgg tcgcgtcgag
     6181 aatgtgatgg aagagtacca tgctattttt gctgagcgga tgattcagca cccggcggta
     6241 cagcgaatct gcaatacgga ttattctgcg ctttttagtc cagcggcgcg ttaatcggca
     6301 gctcccccaa agttaaagtg gaggagatag attagttgta cataaccacg attttgactc
     6361 ggctcattat ttgcccgctt gagacattat ttccatatgt acgcgggcga ataaatagag
     6421 gaatctgatt acttccttca tggggatgct gaaaagagta gtaattgctt ttaccatact
     6481 ggatttcatt cgtaccatca ttggagagtt gaaagcctac ttgacccgca ccgtcctcac
     6541 tgttttctaa ctcatttttt aatatgctgg ttcctgactc ctgtatcgtc actggttcaa
     6601 agtaatattc aacatcactg ggttcaaaaa ctaattcacc ttgctcgccc tggatgcagt
     6661 caaatcttat atgatgaaac tgataatttt cattattgat acgagacagt acaaatcgtc
     6721 cagtgtcgat ggattgatct tcaaaaccac aggtcattaa atttaccagt aaaatagtat
     6781 ttaaattata attaaagcgg
//
LOCUS       DADLFA010000192         6794 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000192 DADLFA010000000
VERSION     DADLFA010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6794
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.194"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(199..1917)
                     /gene="proS"
                     /locus_tag="JQH65_003520"
     CDS             complement(199..1917)
                     /gene="proS"
                     /locus_tag="JQH65_003520"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAY6140808.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(2028..2735)
                     /gene="tsaA"
                     /locus_tag="JQH65_003521"
     CDS             complement(2028..2735)
                     /gene="tsaA"
                     /locus_tag="JQH65_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HAY6140809.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYHKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(2732..3136)
                     /gene="rcsF"
                     /locus_tag="JQH65_003522"
     CDS             complement(2732..3136)
                     /gene="rcsF"
                     /locus_tag="JQH65_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HAY6140810.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(3254..4069)
                     /gene="metQ"
                     /locus_tag="JQH65_003523"
     CDS             complement(3254..4069)
                     /gene="metQ"
                     /locus_tag="JQH65_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HAY6140811.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNSFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(4109..4762)
                     /gene="metI"
                     /locus_tag="JQH65_003524"
     CDS             complement(4109..4762)
                     /gene="metI"
                     /locus_tag="JQH65_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HAY6140812.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(4755..5786)
                     /gene="metN"
                     /locus_tag="JQH65_003525"
     CDS             complement(4755..5786)
                     /gene="metN"
                     /locus_tag="JQH65_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HAY6140813.1"
                     /translation="MIKLSNITKVFHQGTRIIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            5974..6546
                     /gene="gmhB"
                     /locus_tag="JQH65_003526"
     CDS             5974..6546
                     /gene="gmhB"
                     /locus_tag="JQH65_003526"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HAY6140814.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFVLVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAAAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPV"
BASE COUNT         1671 a         1756 c         1728 g         1639 t
ORIGIN      
        1 gctttgatgc tggaatttga cagcccatgt ttttgcctcg gcatctactg ctgtggctga
       61 tggggctggc tgatatcaac ggtacaagcc gaaagtaaaa gaaagctggg gagaagcagg
      121 cggcagtacg cctttggttt atccatttta tacaatccat gtaaaaaagg gccctgaaat
      181 tcaggaccct ttctggcatc agcctttaat ctgtttcacc agatattcga cgatgtcacc
      241 agtcttaatt aactgtttct cgccgttgcg acgatattta tattcgatat cgtcgttgtc
      301 gaggttacgg tcgcccagca caatagtgtg cggaataccg atcagttcca tatcagcaaa
      361 catcacgccc ggacgctctt tgcggtcatc cagcagcact tcgatacctt gtgcacgcag
      421 ttcgctgtac agtttctcag caagctcttg tacgcggaag gatttgtgca tgttcatcgg
      481 cagaatcgcc acctggaacg gcgcgatagc gtcaggccat acgatgccgc gttcgtcgta
      541 gttctgctca atcgccgcag ctaccacacg cgttaccccg ataccgtagc aacccatcgt
      601 caggatttgg ttacggccat cttcaccctg tacggaggct ttcagtgctt cggagtactt
      661 ggtacccagc tggaagatgt gaccaacttc gataccacgt ttgatcagca gcgtaccctg
      721 gccatccggg cttggatcgc cagccaccac gttacggata tctgcaactt ccggggtagc
      781 gacatcgcga tcccagttga taccgaagta gtgtttacca tcgatgttag caccagcagc
      841 gaaatcactc atcgccgcaa cggtacggtc aatcaccacc ggaatcggca tgtttaccgg
      901 acccagtgaa cccggaccgg ctttaaccac agcacgaatt tcttcttcgg tcgcgaaagt
      961 cagcgggctt gcaacctgcg gcagtttttc tgctttaact tcgttcagct cgtgatcacc
     1021 gcgcaccagc agcgcaacca gcgggaagct gctgccttca accgctttaa ccagcagagt
     1081 cttaaccgtt ttctcaatcg gcagattgaa ctgttcaacc agttccgcga tggttttcgc
     1141 gttcggcgta tcaaccagcg tcatttcctg ggtagcagca gcgcgcggtt ctttcggcgc
     1201 gatagcttct gccagttcaa tgttcgctgc atagtcagag gtgtcggaga agaccacatc
     1261 gtcttcaccg ctctgcgcca gcacctggaa ttcgtgagag gcgctgccgc cgatagaacc
     1321 ggtgtcggct tgcacggcgc ggaaatccag ccccatgcgg ctgaagattt tgctgtaggc
     1381 cgcatacatt gcatcgtagg tttcctgcag ggattcctga gaagtatgga aagagtaagc
     1441 atctttcatc aggaattcgc gggaacgcat gacgccgaaa cgcggacgca cttcgtcgcg
     1501 gaacttggtc tggatctgat agaagttcag cggcagctgt ttgtaagagc taagctcgtt
     1561 acgaatcagg tcagtgataa cttcttcatg agttgggccg agtacgaacg gacgctcgcc
     1621 acggtcaaca aaacgcagca gttccggacc gtactgttcc caacgaccac tctcttgcca
     1681 caaatcggct ggctgaacca ccggcatcga cacctcgatt gcaccggcgt tgttcatctc
     1741 ttcacgcacg atgttttcga cttttttcag aacgcgcacg ccggtcggca gccaggtata
     1801 taacccggag gccagcttgc ggatcatccc ggcgcgcagc atcagctgat ggctgatcac
     1861 ctcggcgtcg gcaggtgtct ccttgagagt ggagagcagg tattggctag tacgcatgtt
     1921 gttacggttc cagttggaag gtagaacagg ctcaaggcga gcctgggaca aaaaaagtga
     1981 tttagtttac cagtgcaaaa gaaatgtcaa aagagaaggg cgtgaattta acgcggttcc
     2041 agcgcaaaga cttcaaaacc tgcgtcggtg acgcgccagc gaacgttaaa atcatgtagc
     2101 cagacggcat aggttttgcc cgtttcctca cctttatgat aggccgggcg cgggtcctgc
     2161 gccagtactt cgcggataaa cagcgttaac tgcggataac gcttctccag cgtcaaaagc
     2221 tgcttttcga cctctgcggt aaaactcacc gccatctctg cagctggcgc actttgcgca
     2281 tagctggcac tggcatcggg aagcgattcg gcaaagggga gatacggttt gatatccact
     2341 accggcgtac catcgaccag atccagactg ccgagcttca gaatcacgct gtctttatgg
     2401 caaacaacct ctttcagctc taccagcgac ataccaattg ggttagggcg gaaagtagag
     2461 cgtgttgcga aaacccccat tctggcgtta ccgccgaggc gcggtggacg cacagtcgga
     2521 cgccagccgc cttccatcgt ttgatgaaag acgaaaagga tccataaatg gctgaacgct
     2581 tccaggccgc gaacggcgtc ggcctggttg tagggagcaa tgagatgcag ttcgccgttg
     2641 gcgcttttta ccagacctgg ctggcgcgga acggcgaatt tttctttata gggcgagcga
     2701 ataacgccta tttgctcgaa ctggaaactg ctcatttcgc cgtaatgtta agcgcagagc
     2761 cgatacatac agcctgacga tagcagcctg gcgtaccgct ggtgacttcg cagctatgca
     2821 gtaataccgc attggctttc attttagagg cgttgatttg catccgctta cgcgcggttg
     2881 gaatgctcgg cggagagtcc tgattagagg cctggcaaga gtcgccactg acttcaccga
     2941 gatcgcggaa cggtttgccg actaattctt ctgcattggt ataaattcgg accggcgtgg
     3001 cgcgcggcgc tttcggtttt gcaggctccg ctttcggctg gggtgcagtg ctttgaacgg
     3061 gttcgacagg ggatctgctt aacatggaac agccgcttag catgagtgct actaaacaga
     3121 tcggtaaagc acgcatagta tttcctcaat gtatgatcaa aacgtcaata ttgaatcagg
     3181 agcttgtaaa aatgacaaga cgggcaagtg cccatcctga atgatattac aaattgtgga
     3241 aacagcctaa aaattaccag cctttaacag cgccgccgtt aaacactttg tttgctgctt
     3301 cgtaaacttc atcagactga taagcctgga cgaatttctt cacgttctcg gcgtctttgt
     3361 tatcttcacg cgttacgatc aggtttacgt acggggagtc tttatcttcg acaaagatac
     3421 cgtctttcgc tggagtcagg ccaatctggc tggcataggt ggtattgata actgccagag
     3481 cgatttgtgc gtcgtccaga gatcgaggca gctgcggtgc ttccagctca acaattttca
     3541 gattttttgg gttctcaaca acatcaagta cggtcggcag caggccaacg ccatctttca
     3601 gtttgatcaa gcccactttt tgcagcagca gcagtgaacg accaaggtta gttggatcgt
     3661 ttggcacggc aacctgcgaa ccatcctgca gttcatccag tgatttgatt ttcttggagt
     3721 aaccagcaat cggataaaca aaagagttgc ctactgcgac cagtttgtag ccacgatctt
     3781 tcagttgctg atcaaggtac ggtttatgct ggaaggcgtt ggcgtcgata tcgcctttgc
     3841 tcaatgcttc gtttggcaga acatagtcgt tgaaggttac cagctcaacg tccaggccat
     3901 atttgtcttt cgcaactttc tgcgcgactt ctgcaacctg ctgttcggca ccaacaatca
     3961 cgccgacttt aatgtggttt ggatcttttt catcctgacc gcagcctacc agtgccagtg
     4021 atccaatcag ggctcccact gccgcaaagg ttttgaattt gaacgccata ccttattcct
     4081 tcttcaatta tttatgttgt gttgaacgtt acttgcgagt gacagcccgg acgatgcggt
     4141 cgcctgcgaa ctgaattaaa taaaccagaa tgaccagcaa taccagtacc gtattcatca
     4201 ccgtcgcgtt atagccgatg tagccatact gatagccaat ctgacctaaa ccaccggcac
     4261 cgacagcacc acccatcgcg gaataaccga ctagggtaat cagggtgata gttgccgcat
     4321 tcaccagacc cggcaatgct tccggtaaca gcactttacg gacgatctgc atcggcgtgg
     4381 cacccattgc acgggaagct tcaattaacc cggttgggat ctccagcaga gcgttctcga
     4441 ccatacgggc aataaacggt gctgcaccaa cggttaacgg aacaatcgct gcctgcaaac
     4501 caatcgatgt accaacaata acgcgggtaa acggaatcat ccataccagc aagataatga
     4561 acgggataga acggaaaatg ttcacaatcg cagaaacggt acgatacagc ttcgcgttag
     4621 caataatttg ccccggacgc gtgacataaa gcagaacgcc aaccggcaga ccaatcacaa
     4681 agccaaaaaa accggatacg aaggtcattg ccagcgtttc ccatacgcca cgaaccagca
     4741 gccacatcat cggctcagac ataacccagt acctctactt ttacatggtg ttcctgcagc
     4801 caggcaatgg cggcttgcgt atcttgttgt gtgccgtgca tttcagtcag catgatgccg
     4861 aacttaacgc caccggcgta gtccatctgc gcgctaataa tgttgttgtt gacgttgaaa
     4921 cgacgcgcgg tttcagaaag cagtggggca tcgaccgatt gaccggtaaa ctccagacgc
     4981 agcatcggca cgcagtcagt aaatggctcc gcttgcagac gttcctggta atcttccggg
     5041 atatccagat gcagggtcga ctgaataaac ttctgcgcca gcggcgtttt aggatgcgag
     5101 aacacttcac ttaccgtgtc ctgctcgatc agttcgccat tgctgatgac cgccacgcaa
     5161 tcacaaatac gcttcacaac gtccatttcg tgggtgatca acagaatcgt caaacccaga
     5221 cggcggttaa tgtctttcag cagttcgaga atagaacgtg tcgttgccgg gtccagcgcg
     5281 ctggtggctt catcacacag caatactttg ggattgctgg ctaatgcacg ggcaatcgcc
     5341 acacgttgtt tctgcccacc ggaaagattc gacgggtagc tatcatgctt atcgccaaga
     5401 ccaaccaatg acagcaattc cgtcacgcga cgtttgatct cgtctttcgg tgtgttgtcc
     5461 agctccagcg gcagagccac gttgccaaaa acagtacgcg aagagagcag gttaaaatgc
     5521 tggaaaatca taccaatctg gcggcgagct ttggtcaact cggattctga cagcgtggtc
     5581 agttcctggc catcgaccag cacgctaccc tcggttgggc gctccagcag gtttacacaa
     5641 cgtataagcg tactcttacc cgcgcctgag gcaccgataa cgccataaat ttgtccagct
     5701 ggcacatgca ggctgacgtt gttcaacgcc tggatgatgc gggtgccctg gtggaacact
     5761 ttggtgatat tcgaaagttt tatcattgat tatttattat cgtcattaag ttagtcgtgg
     5821 catctcgaat gcctgaaacg ggcaacgccg tcaatgaaat ggatgttaag gcatccagac
     5881 gtctaaatca atcaggttta tgcgaagagc actttcttgc aggtcgaaac atgcgatact
     5941 agcgtcacat gccttattaa ggagctataa aaggtggcaa agagcgtacc cgcaattttt
     6001 cttgaccgtg atggcaccat taatgtcgat cacggctatg tccatgagat cgacaacttt
     6061 gaatttatcg acggtgttat tgacgccatg cgcgagctaa aaaaaatggg ctttgtgctg
     6121 gtggtagtaa ccaaccagtc tggcattgct cgcggtaaat ttaccgaagc acagtttgaa
     6181 acgctgaccg agtggatgga ctggtcgctg gcggaccgag atgtcgatct ggatggtatc
     6241 tattattgcc cgcatcatcc gcagggtagt gttgaagagt ttcgccaggt ctgcgactgc
     6301 cgtaaaccac atccggggat gcttttgtca gcacgcgatt atttgcatat tgatatggcc
     6361 gcttcttata tggtgggcga taaattagaa gatatgcagg cagcggctgc ggcgaacgtg
     6421 ggaacaaaag tgctggtgcg tacgggtaaa cctattacgc ctgaagcaga aaacgcggcg
     6481 gattgggtgt taaatagcct ggcagacctg ccgcaagcga taaaaaagca gcaaaaaccg
     6541 gtataatgat taaaagatga gcgattgaaa taaaaatgca tttttccact tgtcttcctg
     6601 agacgactcc ctataatgcg cctccatcga cacggcggat gtgaatcact tcacacaaac
     6661 agccggttcg gttgaagaga aaaatcctga aattcagggt tgactctgaa agaggaaagc
     6721 gtaatatacg ccacctcgcg acagtgcgct aaagtgcgtc gcaactgctc tttaacaatt
     6781 tatcaggcaa tctg
//
LOCUS       DADLFA010000193         6773 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000193 DADLFA010000000
VERSION     DADLFA010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6773
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.195"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            147..875
                     /gene="mpaA"
                     /locus_tag="JQH65_003527"
     CDS             147..875
                     /gene="mpaA"
                     /locus_tag="JQH65_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HAY6140815.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(850..1815)
                     /gene="ycjG"
                     /locus_tag="JQH65_003528"
                     /pseudo
     CDS             complement(850..1815)
                     /gene="ycjG"
                     /locus_tag="JQH65_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
     gene            1934..2440
                     /gene="tpx"
                     /locus_tag="JQH65_003529"
     CDS             1934..2440
                     /gene="tpx"
                     /locus_tag="JQH65_003529"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HAY6140816.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCATSVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAFGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(2484..4025)
                     /gene="tyrR"
                     /locus_tag="JQH65_003530"
     CDS             complement(2484..4025)
                     /gene="tyrR"
                     /locus_tag="JQH65_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HAY6140817.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(4173..5234)
                     /locus_tag="JQH65_003531"
     CDS             complement(4173..5234)
                     /locus_tag="JQH65_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HAY6140818.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREICRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(5231..6628)
                     /locus_tag="JQH65_003532"
     CDS             complement(5231..6628)
                     /locus_tag="JQH65_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HAY6140819.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGILRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
BASE COUNT         1615 a         1930 c         1658 g         1570 t
ORIGIN      
        1 taatatctgc acatcaataa aaatgcaaac aacttccggc aaaaaaagtg atacaagatc
       61 gatgacatag cgttgaagaa aaggatttga taatccgcaa aatttgcaca acactgcctg
      121 taatacttcc tgaaccagag acgatcatga ccgtaacccg cccacgcgcc gaacgcggcg
      181 catttccgcc cggaacagaa cattacggac gttcattatt gggcgcgccg ttaatctggt
      241 ttcctgcccc tgcagccagc cgcgaaagtg gtttgattct ggctggcact cacggtgatg
      301 aaaactcttc ggtcgtcacg ctctcctgtg ctctgcggac gttgacacct tctttgcgcc
      361 gccatcatgt ggtgctgtgt gtgaatcctg acggctgcca gctggggtta cgggccaatg
      421 ctaatggtgt ggatttaaac agaaactttc cggcggcgaa ctggaaggaa ggtgaaacgg
      481 tttatcgctg gaacagcgcc gctgaagaac gtgatgtcgt attactgaca ggtgacaaac
      541 ctggctcaga acctgaaacc caggcgctgt gccagcttat acaccgcatt caacctgcct
      601 gggtggtctc attccacgat ccactggcct gtattgaaga tcccagacac agcgaattag
      661 gcgaatggct ggcccaggcg tttgaattgc ctctggtaac cagcgtcggt tatgaaacgc
      721 ctggctcttt cggcagttgg tgtgcagatc tcaacttgca ttgcatcacc gcagagtttc
      781 cgcccatctc ctccgacgaa gccagcgaaa aatatctctt tgcgatggca aacttgctgc
      841 gctggcatcc taaagatgca attcgcccgt cgtgaactga agcgccggtt ccacatctac
      901 cgccagccag gtcggtccgt caagatcggc gaaactgacc tgcggcacca gcggtaaagc
      961 ggcgctaatg gcacgagagg tacacaacat gcagcccagc atcagactga aaccttgtgc
     1021 acgcgcttca gtcgccagcg ccagcgcttc cgtcagacca ccggttttat cgagcttaat
     1081 gttaaccatc tcatagcgcc ctttcagcgc cttcagattg ctacgtgtat gacaactctc
     1141 atcagcacaa atcggcaacg gatgaataaa attctccagc gccgcatcgt cctgcgcagg
     1201 aaggggttgt tcaagcatcg caacgcctaa atccgccaat agctggcaac gcgccgccaa
     1261 cccttctgca cgccaggatt catttgcatc aacgatcagc gtcgcatcgg gcacagctgt
     1321 gcgaattgcc accatccgct cactaataag atggttatcc agcttcactt tcagtaattt
     1381 cgcgcctgcc tgccagagtg ttgatgcact attggccatc tgatcaggcg taccgatgac
     1441 taccgtctgt gcagtgataa ctgtctcggg aagcgttatg ccgatcaaat cagccagcga
     1501 ttgctgctgt tttcgcgcgg ccagatccca caatgcacaa tccagcgcat tacgtgctgc
     1561 gccggcaggg agaatttttt gcaactcctc ccgtgtcagc cctttctcta attgcgacac
     1621 gacgctcata atttgcgcca ttaccgaggc atcactttct ccataacgcg gatacggcgt
     1681 gcattcgccg gtgcctttaa taccctcttc ttccagttca accaccacca cgcgcgcttc
     1741 actgcgactt ccccgggcaa tcacaaacgg ggtatgtaag ggctaggctt cctcaaatac
     1801 cttaacggtt ctcatcttta ctccttgttg tcgcggtggt ctgcggatta agcgcaaata
     1861 gcgtttgctg tgttattgac agttagcata aactaggtgt gacgttaact atatgtaaac
     1921 aggaaagata atcatgtcac aaaccgttca tttccagggc aacccggtta cagtcgccaa
     1981 ttccatcccg caggcgggta gcaaagcgca gacttttact ctcgtggcaa aagatctgtc
     2041 tgacgtcacc ctcggtcagt ttgcgggtaa acgcaaagtg ctgaacattt tcccgagtat
     2101 tgataccggt gtttgcgcca catcagtacg taagtttaac caactggcaa ccgagatcga
     2161 caacaccgtt gtgctgtgta tttctgccga tctgccgttc gcccagtctc gtttctgcgg
     2221 cgcagaaggt ctgaacaacg ttatcaccct ctccactttc cgtaacgctg aatttctgca
     2281 agccttcggt gtggcaattg ctgatggccc actgaaaggt ctggcagcgc gtgccgttgt
     2341 ggttattgac gaaaatgaca atgtgatttt cagccagctg gtggatgaaa tcaccaccga
     2401 gccggattac gaagcagctc tggctgtact gaaagcataa gttaatatgc ctgatggtgt
     2461 tgcaccatca ggcatattcg cgcttactct tcgttcttct tctggcttaa gccatattcc
     2521 cgcaacttat tggcaatcgc ggtatgtgaa acgccgagac gttttgccag tttgcgcgtg
     2581 ctgggataat tgcgataaag ctgggttaat accgagcgtt caaaacggct ggtgatttcg
     2641 tccagcgaac cttccatcgc atcttcgccc acggctaccg ttgcggcgtc ataatccggc
     2701 aacaaaatat cctgtggacg cagctcataa ccgtccagtt gtgtcagtgc gcgatatata
     2761 gcgttcttta actgccgcac atttcccggc cacgcataac gcgtaagtac agtattcagg
     2821 tcagcggcca gtttcggacg cggcacgccc tgctcgtcgg caaagcgggc gacgaacagc
     2881 tcagttaacg gcatgatgtc ctgcggacag tcacgtagcg gcggcagatt gagcgtcaac
     2941 acgttcagac gataatagag atcttcacgg aacatgcctt tttgcaccag ttcgaccaga
     3001 ttcttctgcg tagcgcaaat cacccgcaca tcgacatgca cctcatggtc ttcgccaacc
     3061 cgacggaaag tgccatcatt aagaaaacgc agtaatttcg cctgcatccg tggtgacatt
     3121 tcccctattt catccaacag caccgaacca ccgttcgcct gctcaaagaa tcctttcttc
     3181 ccttccggag catgaccaaa cagttcactc tcgaccgcat cttccggtat agacgcacag
     3241 ttcagcgcca ggtaaggttt gcccgctctg gggcttgcct gatggcaggc gtaggcaaag
     3301 agatctttac ctgtacctgt gtcacccgta atcagcagcg gcgcgcttag catcgccagt
     3361 ttctgcgcct gttcgacaac atgcttcatt ttcgggctga cggcgacaat ttgactgaag
     3421 gcgctgacgt cctgggcggc gacattttgc aactggcggc ccatacgaat cgttgatcgc
     3481 aacatcacca ccgcaccggt caggacgtgt tgatcatttt catcctgaag ataaacaggc
     3541 gtaatctcca tcaggaaatt ctgcccatta ataacgacat gctcgttatg cgaatcttgc
     3601 ggttcgcttt ccagccaacg taaaaaatta aagccgttaa tcaattgtgc ggcggtatgg
     3661 ttgcgcaggc gatccaattt ttgcccaaaa agctgacagc tcgccgggtt cgccatatcc
     3721 actttgcttt tcatatcgac agagagcaca ggttcgggca acgcctccag taacgcgctc
     3781 aacgccagat gctcacgttc ggaaggcatc cacgggacag tacgcacatc ggtaacacct
     3841 gcaatacggc gtatttcggc catcagactg ctgaaactct caaactccag ttcagcaaaa
     3901 ttgaggtaga ttcgcccaat gggatcaatc tcaataccgc gtaaatcaat gcctcttagc
     3961 acgagtagat cgagtaattc gcgggtcaga ccgagtcggt cttcacaaaa gacttccaga
     4021 cgcatgggaa ccttcacctg aaaaaagaac aattaatatg atgatatgac actatttgga
     4081 tagcaggaag gtttctgtca acaatcattg acacaaagac ggaaaaacgg gcacttcggc
     4141 gtaaagatat ccggtcagca cccgatattg cattattttt cgctgggcgt tttgcctttt
     4201 tgcagcgttt ctttcacctg accgataagc tgacgacgga aatccccgag gcgaggtttg
     4261 tcatcgtcaa tccacggcag cgggcggcaa agctccatag ctttaatccc gagtcgtgcc
     4321 gtcagaagtc cagcaccaat tccctgagct gcgcgggtag acaaacgagc agcgagatct
     4381 tgcgacatcc agtccatccc cacttcgcgc accagttcgc tggctccggc aaaagcgata
     4441 ttcagcaata ccagcttaaa cagacgcaaa cggctgtaat accccagttc aatgccatac
     4501 agcgtggcga tgcgattaat taaacgcaga ttgcgccagg cgataaacgc catatcgacc
     4561 aacgccagcg ggctgaccgc aatcatcaac gttgattccg ccgccgaacg gcagatttcg
     4621 cgccgcgcct gggcatctaa aactggctgg accaaatgcg catacaaact gaccacttca
     4681 cggtcgtttt gcgtttcatg gattgaggca taccagcgtt gcagcgctgg atgagactga
     4741 tcaatacccg cctgctgcgc cagtttttcg caaaatgcgc ggcctttgcc cgtgccgtgg
     4801 ctgtgcaaca aatcgcgcgc ttcgtcgcgt tcatgggcgc gctgtcgcaa gcgccataag
     4861 cgccgccact ctgttaccac agaacctacg ccagcgccga taatcaatgc cccagcggca
     4921 catccaccca gcgccaccca gtcctgagtt tgccaggcat tcattgtcca ctgtacaccc
     4981 tgcccgacaa cgcttgcgcc aaacagagcc agcccgccca tcaccatttt gcgccacagg
     5041 ctgcgtttcg gacgtaacgc tgcatccatt accgcttcaa cttgcccctc ttcttcaggc
     5101 gcttcatcga gcgtggccgg ggcaaaattt tgcgcctgat tttcgtcaaa ggtctgctgc
     5161 gccctgaatt ttggattctg atcgacctcc agcggaccgt cgaaatcaat acgtggtttt
     5221 aacggttcgg tcatcgcaat ttatctccta ttaaaaattc cagcgcggca tcaagacgaa
     5281 tatgcggcag cggtttgtcg acatccatca cctgcgggcg aaacgcttca aactggaacc
     5341 cttgtttgtc ccagaacgcc tgaccgggca aacgcgcagg aacttcaccg ggataaacgg
     5401 tgagtggcgc accatcgctg agccggttac cgcgcaatgc cggaattttc tcgccgttga
     5461 cgtcaataat gccgctggtg gtcgcctgca ccgaggccag ccccaggcaa tccatactaa
     5521 tcccttcaaa cgccgcattt tgccaggcat cctgaatcag ttgttgcagt aatgaaacca
     5581 tattggcgtg ctgatcgatg gtcacatggt ccgctttagt ggcagcaaac aatagcttat
     5641 cgataaccgg cgaaaacaaa cgccggaaca aggtacgctg cccgtagtgg aaactttgca
     5701 tcagctgcgt cagtgccaga cgcatatcat taaatgcctg tggcccactg ttgagaggtt
     5761 gcaggcaatc aaccagcaca atctggcggt caaagcgcag aaaatgattc ttatagaacc
     5821 ccttcaccac cttctcgcag taataattaa accgctcgcg cagcattccg gcattggtgt
     5881 gcttatcggc ctgcgccagt ttggactcgc cccaggtatc gacatccggc cacgggaaga
     5941 attgcagcgc gggcgcacct gccatatctc ctggcaagac aaagcgccct ggctgaataa
     6001 agtgcagccc ctgctgttta cagtggtgga gataatcggt ccacgcggcg gcaatgtctg
     6061 ccagccggtt ttcgtcggca ggtgctagcg ggtccagccc ttcgcacatc attcgccatt
     6121 tgaccgacca ttcgccgcgc tgaccattga gtaagcccgt catctggcgt gaccagctta
     6181 aatagtcctg cgccagcatc ggcaggtcga gcaaccattc gccgggataa tccacaatct
     6241 ccagatacag cgtggaggtg tccttgaagt ggcgtagcag cgaatcgttc gatttaaaac
     6301 gtagcgccag gcggatttca ctgacgccgc gcgttggcgt cggccaggcg ggaggatcgc
     6361 catataactg cgccagtcct tcatcatatg tgaagcgcag aatgccaaag tcacgctgag
     6421 gaatgcgttt tacgcccagc aggcgctctt cacgcaccgc gcttaacagc ggcaagcgtg
     6481 ctccggcgtg aatattgagc aactgattga ccatagcagt gataaacgct gttttgccgc
     6541 tgcggcttag tccggttacg gcgaggcgca gatgtctgtc gacaccccga ttcaccagcg
     6601 cattaagttc atttttaagt cgcttcattg ctgtcctgtg ctgctctgaa gaccagaaaa
     6661 aaatcattcg taaggatatg aatgtggggg ccaggccatt gattatcaac tgcccgtgtt
     6721 gtcatcgacg ggcgaactcc ataagatgtg cagcattcac gttcggacgg agt
//
LOCUS       DADLFA010000194         6759 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000194 DADLFA010000000
VERSION     DADLFA010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6759
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.196"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..297
                     /locus_tag="JQH65_003533"
     CDS             <1..297
                     /locus_tag="JQH65_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY6140820.1"
                     /translation="LPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVT
                     FANVFTIIPPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(448..3771)
                     /gene="mscM"
                     /locus_tag="JQH65_003534"
     CDS             complement(448..3771)
                     /gene="mscM"
                     /locus_tag="JQH65_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HAY6140821.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERTREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(3793..4761)
                     /gene="psd"
                     /locus_tag="JQH65_003535"
     CDS             complement(3793..4761)
                     /gene="psd"
                     /locus_tag="JQH65_003535"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HAY6140822.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLKALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(4858..5910)
                     /gene="rsgA"
                     /locus_tag="JQH65_003536"
     CDS             complement(4858..5910)
                     /gene="rsgA"
                     /locus_tag="JQH65_003536"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HAY6140823.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNELLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHSGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            6005..6550
                     /gene="orn"
                     /locus_tag="JQH65_003537"
     CDS             6005..6550
                     /gene="orn"
                     /locus_tag="JQH65_003537"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HAY6140824.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDSFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
BASE COUNT         1563 a         1814 c         1812 g         1570 t
ORIGIN      
        1 cttccttacc tgttcctcgc gctggctttc ccattcttta aagcacgtca ggatctcgac
       61 agaccatttg tgattttcaa aacgcgtatg tcggcaatga ttgcgacggt ggttgtcgta
      121 ctggtggtga catttgcgaa cgtcttcacc attattccgc ctgtggttga agccggagac
      181 tgggacagca cattgtggat gattggcggc cctgtcttct tctcgctgtt agcgatggcg
      241 atttaccaga actattgcag tcgcatggcg aataaacctg agttagctct cgactgatca
      301 tatgaaatcc tgcaactcat cgtctctccg gcgatgagtt gtcaggaagt aatgcgtatg
      361 gatgatactc tccagggccg gtgataactg aagatgatgt taagctgcta aatgagccac
      421 gttgacatca tttggcagct tcatgcatta caaacttccc gcctgacgac ctttgcccgc
      481 agacgtcagc gttctccccg tttgtttacc gttgaggctt tccagacgca tctggaaggg
      541 cgggaatggc atatcgatac cgtgggcatg gaagccagcc agaatcagct ggtggatctc
      601 atggcgtagc ggcatacggt gacccatctc agcggcataa atacgcagct cgaaaatctg
      661 aatcccttgt tgcagatcca ccaggaagac ttccggtgcc gggttgtcga tcaccagcga
      721 gcagcgacgc gctgcggtga gcaggatttc cgtcacttct tcactgttgg catcagcagg
      781 ggccggaatc gtcaacacta cgcgcgtaac cgagtcagag agcgaccagt tgataaactg
      841 ctcggtaata aacgccttgt tcggcacgat tatctcttta cggtcccagt cgctgatggt
      901 ggtggcgcgg gtgttaattt tcgtcacgct accggtgaga tcgcgaattg tcaccgtatc
      961 gccaatgcga atcggttttt cgaacaggat aatcagacca gagataaagt tggcgaaaat
     1021 ttcctgcaaa ccaaaaccga gaccaacacc gagcgcggcg accagccact gcaatttcga
     1081 ccattcaata ccaatcatcg agaagccgac cagcccgcca atcagcatca gcagatattt
     1141 ggtgatggtg gtgatggcgt agcctgtacc cggcgttaaa tccaggtgct gcaaaatcgc
     1201 cagttccagc agcgcgggca ggttgcgcac cagctgcgtg gtgatgataa acaccagaat
     1261 ggcaatcagc accgcaccga gggtaattgg ctccagactt tctacgcctt gtaccgtgga
     1321 ggtgacatcc cacagcgaaa tattttcgag gaagccgaaa gcggaatgga tttctgacca
     1381 cagcacaatg acagaaagca gggcgatcaa catcaaaatt gagcgcacca gccgcaagga
     1441 ttgcgcactg atggcatcga gatcgacttc gctttcatcg acttcaattg ctccttccgg
     1501 gctactgtga tgatgcgctt cctcttcgcc acgcgcacgt tgcgctaaca tctctgcccg
     1561 gcgatgcttc gcccggtcaa acgccagcct gcggcgctgg atcagcatcc agcggcggat
     1621 aacgtgataa accaccagta gcaggaacca gatggcaacc gaggtttcaa gcctcgctaa
     1681 cagcgcctgt gccgttgcca gataacccac agccgacgcc agaatggcaa ccaacggcgc
     1741 gccaatcatc atgttccaca gcatatggtt ggtaatgttg tcgccgctgc cctctttgtt
     1801 gagatacagt gggatcccgg cctttttcag gctgagggtg accaccgcta gcgcaccgca
     1861 aatgaggata aagcaaagcc gtcccagcga accggagaat tcacggtcgt cgaggttatc
     1921 gaacatcatc agcgccataa tcagcggcac aataagcccg atgctcatca ggtagtagcg
     1981 catcccacgg gaaacacgtt cgcgcggcca gccaaaatga gcgataaaca agccgttcgg
     2041 gcgggcaaag gtggcgcaaa tcatcactac ccacagcagc ggcacggtag ccgttacacc
     2101 atcgccaatc gcgaccgcca gcggataagg ccacgcctcg cgcaagccgt aacccagcgt
     2161 catccacagc accggtaacg gtgacgcgac gagaatcgac cagaaaagag tgcgcaacgt
     2221 cagccagaag tgatcctgag tcactttgcc aactttcgcc gccgaacgtt caagaaaacg
     2281 ggtgaaatag cggcgcgagt aaatactgca accgaccaga atcaacgcgc caaacagcgg
     2341 caaaattgtc tctttgctgg tcagcatcat cacactggct ttgcccaact gactgaaggt
     2401 gtccagcgaa atgagacgac gcagatcctg ggcgatttcc agcggccagg cgatggtcat
     2461 cgggcgcacg tcagaggtcc agaacagata gcggtgcgtt gcttcgttca cctctttcag
     2521 cgcatcctcc agttgcccgt tggagacttt cagcttggtc agttccaata gtagcgtgtc
     2581 gccaccctgc aacaatgagt tcagcaactc acgctgagtg cgcagttgtg cttccagaat
     2641 acggttttgc tcggcggtca gcggctgacc gtcggcctga tggatttgcc gtagcagcgg
     2701 ctgtttatta agcagatcct cataacgtaa ccgttgcaca cgcaactgcg ccatttcggt
     2761 atcaagctgt tgtggttttg gcatttctgg cagccgtgcc acctgcgccc gcagcgcttc
     2821 gccgagcaga ttggacgatc ccagccattg cgactgttca cgcagcgtat tcaacgcctg
     2881 ccggacctgt aacgtctggc tggcagcctg acgctgttgc gaggcaacga gatccatccg
     2941 ctgcgcctgt tgattcaaag ccgccgatag ttcgcggtta attttgaatt gcgcgacgat
     3001 atctttcggc aaatcggcgc tgttttctgc cagcaattcg gtactttcca gcgcccgctc
     3061 cgcctcaagc tgacgttggc tgtttaattg attacgcaag gcctgcaaat acgcatccag
     3121 ttgctggctc tctttttccg ccagctctga gcgtaagcgc gctaattcct ggcggttatt
     3181 ggcagacagc tgcgccagct ccagttcatc aacgagcgcc ttaagacgtg cagagtcaga
     3241 ctgcaacgcg aaattttgtg cctgattgag cggagtattg ccggtaagcg ttcccaggcg
     3301 gcgctcgatc tcatttaact gacggcgggc gtcggtttgc tgttgcggca gttgattcag
     3361 cgaatcggca atctcgcggg tgcgctcctg ctcttgctgg gcctgacggc ttttatccag
     3421 caactggctg ctgacctgga gaatttcctg attcagcgcg tcggtagaca ttcccggcga
     3481 cacgctgcgc ggctcgtcac gcatgttgtt taattgtgcg cgcagagtag cggagagttt
     3541 cggataatta tcgataactt gctgatattg tttgatgcgc tcaagggaac cttttcgttc
     3601 ctcaagcgca tttaaggcag actggagcgc ctctacgact tccggctgtg cgggtttcgc
     3661 cgcttttgcc tgctccagtt cctgagtgat ttgtttgcta tcgggggccg tcgcggcgta
     3721 cgccccccaa ctgaggcacc aggccatcag aaaagtgata atcaggcgca cgtcagcgtt
     3781 tcctttgatg gattagacct ggtctttttt atcgtcaacc aatgggctgg cgtcgtgttc
     3841 tgcttcgatc tcttcagcag gaagtggggc gggttcagcg tctggcgtaa caaaggtttc
     3901 ggtagatact gccagcggct ggccaatttt cgtgacagac aggctttcca gttgctcaac
     3961 cagattcact ttacccggtg caaacaggtt gataacggtg gaaccgagtt taaagcgacc
     4021 catttcctgg cctttcagca gtgccacaga accgtcgttt tccccggcag gccaggtcca
     4081 gcgcttgatg ataccttcgc gcggcggcgt aatggtgccc gcccagaccg tctcaatgct
     4141 gccaacaatc gtcgctccga ccagaatctg cgccattggg ccaaattcgg tatcgaaaag
     4201 gcaaatcacg cgttcgttac gggcaaacag attcggcacg ttctgagcgg tgagatggtt
     4261 aacggagaag agatcgcccg gcacgtagat catctcacgc agaataccgt tgcacggcat
     4321 gtgtacgcga tggtagtcac gcggggagag gtaagtggtc acaaacgtac cgttgcggaa
     4381 caggtccgcc atcagatagt tgcctgccag cagggctttg aggctgtagt tgtggccttt
     4441 ggcttgcagg attttatctt cttcgatttt acccaactgg ctgataacgc catcggcagg
     4501 catgaccagt acattcggat cggtatcgat tgggcgtact tcgtcacgca gcggacggac
     4561 aaagaattcg ttaaaggtgc ggtagctggc ggtgtccggc ttttgcgcct ctttcatgtc
     4621 gaccttgtag tatttaacga acagatcgat aaccagtttt gtcagccatc ctgcccgctt
     4681 gcttgcgccc caacccgcca ggcgagtaag ccatagtttc ggcagaatgt actgtagcga
     4741 aagtttaaat gaatttaaca aggtagcctc caggccattg ttttgtcgtt cctgatccgg
     4801 ccaacatgcc ggatcctgaa aaaaagggga cgattctaac gacggttagc ttaattgtca
     4861 gtcatccgta tcagaaaagt ttttacgcgt ttttacctgc gccatgcttt ccagaatacg
     4921 gtgatagttt tcgaaacggg tttccgcgat tttcccttcc tcaaccgctt cccggatagc
     4981 gcagcccgga tcggtatcgt gtttgcaatc gcgatattta cacagaccta aatagtcatg
     5041 gaattcgaca aagccctgag tgatttgttc cggctccagg tgccagaggc cgaactcacg
     5101 cacaccaggg gaatcaatca catcaccgct gtgcgggaag tgatacagcc gagcggcggt
     5161 cgtggtgtgc tgaccgagcc ccgagttgtc agagacatcg ttggtcagga tctctttttg
     5221 cagccccagc aactcattca gcaggctgga tttgccgacg ccagactgcc cggcaaaaat
     5281 gctgatgcgc ccggtcaacg cctcttccag cggttttagc ccatcctgag tatggctgga
     5341 aaccatcaat acgcgataac cgatattgcg gtagatatcc atctgctcgt tgacgaacgc
     5401 catgccttcg tcgtccagca ggtctatctt gttgagcaca ataatcggct caatctgcaa
     5461 ggtttcgcag gccaccaggt aacggtcgat aatattgagt gacagctccg gcaaaatggc
     5521 ggagacaatg acaatctggt cgatgttggc ggcaataggt tttacgccgt cgtagaaatc
     5581 cggacgcgtc aacaccgagg tacgctcatg caccgcttcc acgatccctt tgacatttac
     5641 gccttccgcc gccggtttac ccggacgcca gactacacgg tcgccggtca ccagcgaacg
     5701 gatggtacgg cgaatattgc agcggtgaac gtcgccatcg gcggattcca catcagcgtg
     5761 cataccaaag cggctgatga cgataccttc atcaggctcg ccaaacagat tgtcgtcgta
     5821 gtcgggcttc tccttagacg ttttaagacg acgctggtga ttggcgttca cgcggcgctg
     5881 ctggcctttg gagagtttat ttttactcaa tcgtacagac tcctggtcgc ccctgatggg
     5941 caaaacatct atgatacacg caattgtgga tcaatatagt cactgtgaat gggtggaaaa
     6001 tagcatgagt gccaatgaaa acaacctgat ttggatcgat cttgagatga ctggtctgga
     6061 tcccgagcgc gatcgcatta ttgagattgc cacgctggtg accgatgcca acctgaatat
     6121 tctggcagaa gggccgacca ttgcagtaca tcagtccgat gaacagctgg cgctgatgga
     6181 tgactggaac gtgcgcaccc ataccgccag cgggctggta gagcgcgtga aagcgagcac
     6241 gatgggcgat cgggaagctg aactggcaac gctcgaattc ttaaaacagt gggtgcctgc
     6301 gggaaaatcg ccgatttgcg gtaacagcat cggacaggac cgtcgtttcc tgtttaaata
     6361 catgccggag ctggaagcct acttccacta ccgttatctc gatgtcagca ccctgaaaga
     6421 gctggcgcgc cgctggaagc cggaaattct ggatagtttt accaagcagg ggacgcatca
     6481 ggcgatggat gatatccgtg aatcggtggc ggagctggct tactaccgcg agcattttat
     6541 caagctgtaa aatttttcct ggtcacgtaa gcgccggaat ccacttgcgg cggcgtgatt
     6601 ttgacgctaa attgaacgtc ttgctgataa tttcagcact tgagataaaa acgcaaaaaa
     6661 aacttttttg gggggttgca gagggaaaga tttctcgtat aatacgcctc ccgtaacgac
     6721 gcagaaatgc gaaaattacg aaagcaaaat taagtagta
//
LOCUS       DADLFA010000195         6626 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000195 DADLFA010000000
VERSION     DADLFA010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6626
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.197"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..357
                     /locus_tag="JQH65_003538"
     CDS             <1..357
                     /locus_tag="JQH65_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6140825.1"
                     /translation="AITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDNYIIQTL
                     DVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTQKEAILTAPTHFNKTCITSDC
                     WQILFFSPDHFNNDFY"
     gene            379..1557
                     /locus_tag="JQH65_003539"
                     /pseudo
     CDS             379..1557
                     /locus_tag="JQH65_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhaC family protein"
     gene            complement(1945..2321)
                     /gene="rnpB"
                     /locus_tag="JQH65_003540"
     ncRNA           complement(1945..2321)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="JQH65_003540"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(2354..3499)
                     /gene="garK"
                     /locus_tag="JQH65_003541"
     CDS             complement(2354..3499)
                     /gene="garK"
                     /locus_tag="JQH65_003541"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HAY6140826.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(3596..4486)
                     /gene="garR"
                     /locus_tag="JQH65_003542"
     CDS             complement(3596..4486)
                     /gene="garR"
                     /locus_tag="JQH65_003542"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HAY6140827.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(4516..5286)
                     /gene="garL"
                     /locus_tag="JQH65_003543"
     CDS             complement(4516..5286)
                     /gene="garL"
                     /locus_tag="JQH65_003543"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HAY6140828.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(5302..6600)
                     /locus_tag="JQH65_003544"
     CDS             complement(5302..6600)
                     /locus_tag="JQH65_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005155249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY6140829.1"
                     /translation="MHTRYLILLIIFIVTAVNYADCATLSIAGTEVAKELQLSTVSMG
                     YIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGFVDMFPLAWAGIS
                     MFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFSLALFSPLLGWLT
                     FAWGWEHVFTVMGVIGFVLTALWIKLIYNPTDHPRMSAEELKFISENGAVVDMDHKKP
                     GSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIYLVQEKGMSILKV
                     GLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLLASTIILCNYTNN
                     TTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGNVASIVTPLVIGY
                     LVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
BASE COUNT         1814 a         1726 c         1442 g         1644 t
ORIGIN      
        1 gcaattactg cattgaattt ctatcaaggc gatgttcctt ctgtcatgat ccgaatccaa
       61 ccggaaaata tgagtccatt catcatagat atttctacag gagaacatga taattatatc
      121 atacaaacat tagatgtagg cacttttgca ccttttggtg aacaatgtac ttgctcagcc
      181 gtcaataaaa aagagctgga atgtattaag gagacgattt ctaagtattg cgcaaagttc
      241 acccaaaaag aagccatctt aacagcccct acgcacttta ataaaacctg cattacttca
      301 gattgttggc aaattctctt tttttcaccc gatcatttta ataatgattt ttattgatta
      361 tttataaagg ataaaataat gttccctgtt tcatcaattg gtaatgatat tagtagtgat
      421 ttagtccgtc gaaaaatgaa tgaccttcct gaaatcccca tagaaaataa tctcgaagca
      481 ctagcaccag gtatagaaaa actaaaacag acctctattc aaatggtcac tttacttaac
      541 gcgttacaac caggtggaaa atgtattatc actggtgatt ttcaaaaaga attagcgtac
      601 ttacaaaatg taattcttta taatgactcg tttcttcgtc tggatttttt aggttataac
      661 gcccaaatta ttcaacgatc ggacaatacc tgcgaactta ccattaatga accgttaaaa
      721 aaccaggaaa tatctacagg caatatcaat gctaattgtc cattaaaaga tatttacaat
      781 gaaatcaaga ggttgaacgc aacctttagt tgtgggactg gagatatagt tgatctatcc
      841 tctctggact tacgtaatgt cgatttagat tattatgatt tcacagataa acatatggct
      901 aatactattc taaatccttt taaattggat tcaacaaatt ttactaatgc caacatgttt
      961 caggttaatt ttgttagttc aacacaaaac gccacaatct cctgggatta tttactaaaa
     1021 ataacgcctg ttttaataag tattagcgat atgtattctg aagaaaaaat caagtttgtc
     1081 gaaagttgtt taaatgagct tggagacatt accgaagaac aattaaaaat tatgagattt
     1141 gcaattataa aatctatacc aagggcaact cttacagata aattagaaaa tgaattaaca
     1201 aaagaaatat ataaaagctc atcgaaaatc aacaattgct tgaacagaat taaattacca
     1261 gagatgaaag aattctcatc aaaaaaaaac atatgattac atcgatataa tcattgaaga
     1321 ttatgaaaat atcaaagaaa atgcttatct ggtcgtcccc caaattaatt atactatgga
     1381 tttaaacata gaagactcca gctcagaaga gttaataccc tcgagaaaga cgaaaattct
     1441 tcggacaatg gctttgaagt cgaagaatat aacgcatatg aagcatataa ctcagagaat
     1501 ctatatttta ccagagagga atatacgtat gattacgacc tttcaaatgc aatatagact
     1561 taataacttc ttacaaaatc tataattaat accgtagata ttcattcatc ttaccctgtg
     1621 caccaaaagc aaaaactcac tgacgcgggt ttgaaactga tggcaaccgc aaaaatgcct
     1681 gatgcgctac gcttatcagg cctacgccat ctctgcaata tattgaattt gcgtgctttt
     1741 gtaggcagga taaggcgttc acgccgcatc cggcatgaat aaagcgcact caacaatctg
     1801 aaacccgccg gaacggttta ttacgtacat caggtgaaac tgaccgataa gccgctttct
     1861 tttgggtata gtgtcgtgga cagtcattca tctttctgcc cctccaaaag caaaaacccg
     1921 ccgaagcggg tttttacgta aatcaggtga aactgaccga taagccgggt tctgtcgtgg
     1981 acagtcattc atctaggcca gcaatcgctc actggctcaa gcagcctacc cgggttcagt
     2041 acgggccgta ccttatgaac ccctatttgg ccttgctccg ggtggagttt accgtgccac
     2101 ggactgttac cagccgcgcg gtgcgctctt accgcaccct ttcaccctta cctgatcccg
     2161 cttgcgcggg ccatcggcgg tttgctctct gttgcactgg tcgtgggttt cccccccagg
     2221 cgttacctgg caccctgccc tatggagccc ggactttcct cccctccgcc cgtctccccc
     2281 gaagaggacg acgacgaagc ggcgactgtc tggtcagctt cggcgcgcag tatagagggt
     2341 ttgcgcgccc ttgtcacccc gcgttgcgca ttccaatcgc cagtgtcgcg gcgatattac
     2401 gtgaagcacg gcagatattg tcataagccc cgcggaatgc ttcgtccaac gtacctatgc
     2461 tggtcaatac gctgaagacc gcatcaatgc catgctgatg tacaacgcca acatcatcgg
     2521 tcaggctacc cgcaatgcca atcaccggtt tatggtactt cttcgccacg tttgcgacac
     2581 caatcggtac cttgccgtga atactctggc tgtcaatacg cccttcacca gtgatcacca
     2641 gcgtacaatc gtgaatatgt tcttccagat tcagcgccgt agtgacgatt tcaataccac
     2701 ttttcagttc cgcaccaaga aacgccatca gcgccgcgcc cataccgccc gcagctcctg
     2761 cgccggggac atctttcaca tcaacatgca gcgctttttt aatgacctcg gcatagtgag
     2821 agaggttatt gtccagctca acaatcatcg cttcactggc tcccttttgt gggccaaaga
     2881 tgcgcgatgc gccgttatcg cccaccagcg gattggtgac atcacaagcg acgcgaatga
     2941 cgcaatcttt taagcgcgga tcgaggccgg aaatatcaat atcattcaga gtattaagac
     3001 taccgccgcc aaaaccaatt tcattgccgt tggcgtcgca taatttcgcc cccagcgcct
     3061 gtaccatgcc tgcgccgcca tcatttgtag cgctgccgcc aatgccgata ataatgtttg
     3121 tcgcaccgct ctccagcgcc tgcaggatta actcgcctgt gccgcgtgaa gtggtcacga
     3181 gtggatcgcg tttttccgca ggtaccagct ccagcccact ggccgccgcc atttcaataa
     3241 acgcggtttt gccatcgccg gagatccccc aactggcatt cactttctcg cccaacggcc
     3301 ctgtaaccca ggcgtgacgt tcggaaccct gggtggctgc aatcatcgct tccaccgttc
     3361 cttcgccacc gtcggcaacc ggaacagaaa cgtactgtgc atcaggaaaa atttcccgaa
     3421 atcctttttc tatcgcctgc gcaacctcgc tggcagataa actttcttta taagagtctg
     3481 gggcgattac gattttcata cctatgcctg ttaccacatg acgccggagg gcgtttctct
     3541 tattcggcgt ggattccagg cccggattgc aatacgccat ccgggcacga cgtcattaac
     3601 gagtaacttc gactttcgcc agtttttcgt agtagcacgc cagagcgcta tgatccgccg
     3661 ttcctaaacc atctgcacgc agtgcctgca tcatctccat aaccgcagct gtgagcggca
     3721 gttgcgcgcc gacgccgtga gaagtatcca gcgcattcgc cagatcctta atatgcagat
     3781 caatgcggaa gcccggcttg aagttgcggt ccatcaccat cggcgctttg gcatccagca
     3841 cggtactgcc cgccagccca ccgcgaattg cctgataaac caggtccggg ttaacgcccg
     3901 ctttagttgc cagcgttaac gcttctgaca tcgcagcaat attcagcgcc acaatgacct
     3961 gatttgccag tttggtgacg ttacctgcac cgatttcccc ggtatgcacc acggaacccg
     4021 ccatcgcttt catcaaatca tagtatttgt cgaaaatagc cttgtcgccg cccaccatca
     4081 ctgacagcgt accgtcgatg gctttcggtt caccgccgct caccggagca tccagcatat
     4141 caatgccttt cgctttcagc gcttcgctga tttcacggct tgccagcggt gcgatagaac
     4201 tcatatcgat caataccgta cctggcttcg cgccttcaat aatgccattc tcacccagcg
     4261 ccacctcttt cacatgaggg gagtttggca gcatggttat gatgacgtcg cactgttcag
     4321 cgatcgcttt agccgtagac gctgtttctg cacctgcagc aatcacgtca gcaatagctt
     4381 ctgggttacg gtcagcaacc accagcgagt aacctgcttt cagaaggttt ttactcattg
     4441 gtttacccat aatccccagg ccaataaaac caactttcat agtcatatca atcatctctc
     4501 ttgttgcggt ggtggttatt ttttaaaggt atcagccagt ttctgagtgg cagagcggaa
     4561 gacgccaaga tcgctgccga cagccacaaa cgtcgcgccc cattccagat aacgacgcgc
     4621 atcggcttcg accggcgcga gaataccgct gggtttgccg tgcgcactgg cacggttaaa
     4681 aatatgctga attgcttttt gtacatccgg atgcgaggca ttgccgagat ggcccaatgc
     4741 cgcagccaga tcgctggggc cgacgaagat accgtctaca ccttcggtag cggcaatagc
     4801 atcgacgtta tctacgccct gctgactttc tatctggacc agaatagtga tgttcttgtt
     4861 cgactgagcg aaataatccg ccacggtgcc aaacatattg gcgcggtgag aaacggagac
     4921 gccgcgaatg ccttccggcg ggtaacgagt tgatgccacc gcctgctctg cttcctcttt
     4981 tgtttctaca aaaggaatca ggaaattata gaaaccgata tccagaagac gcttaataat
     5041 taccggctcg ttggtcggca ctcgcactac tggcgcgctg gcgctgcctt tcagggccat
     5101 taactgcgga ataaacgtgg aaatatcgtt tggcgcatgt tcgccatcca gcaccagcca
     5161 gtcaaaccca gccaaaccaa gaacttcagt gctaatcggg ttagagagtg ctgaccagca
     5221 accaatttgt acctgtttcg cagccagtgc ggctttgaat ttattcggga aaacatcgtt
     5281 attcatcgct tatacctttg cttatttctg caattccata cgtttaatgt cgccaactac
     5341 gaagaggtag cagaccatcg ccatcagcgc tgaacatccc acgaaaacca gtgctgcatt
     5401 gaaggagtgc agttcactta ccaggtagcc aatcaccagt ggagtgacaa tggaggcaac
     5461 attgccaaag acgttaaaga cgccgccaca gaggccaaca atctctttcg gcgcggtgtc
     5521 agaaatcacc ggccagccca gagcaccaaa tcctttgcca aaaaaagcca gcgccatcag
     5581 catgaccacc agcgtggtgt tgttggtgta gttacataag atgatggtgg aagccagcaa
     5641 cattcccagc acaatcggta gcttacgtgc cagggtcagg gataaaccgc gtttgatcag
     5701 ataatccgag aagacacctc ccagcacgcc gcccgcaaaa ccacacagtg ccggaatcga
     5761 ggcgaccaga cccactttca gaatcgacat gcctttttcc tgcaccagat aaatcgggaa
     5821 ccaggtgagg aagaaccagg tgatggtgtt gataaaatat tgtccgaaaa atacgcccaa
     5881 catcatgcgg ttagagagca attgcttgat gtaatgcagt ttgggaccgc ttgctgccgc
     5941 gctgcccggc tttttgtggt ccatatcgac caccgcgcca ttttcagaga taaacttcag
     6001 ctcttccgca gacatacgtg ggtgatctgt cgggttataa atcaacttga tccacagcgc
     6061 cgtcagcaca aaaccaatca cccccataac ggtgaagacg tgctcccagc cccaggcgaa
     6121 agtcagccag ccaagcagag gcgaaaagag cgccagcgag aaatattgcg ccgagttaaa
     6181 gatggcggag gcagtaccac gttctttcgt cgggaaccag gcggcgacaa tacgggcgtt
     6241 cgccgggaat gatggcgctt ccgagaagcc gagcataaag cgcataaaga acatagagat
     6301 ccctgcccag gccagcggga acatatcaac aaagccttgc aggaaggtga atagcgacca
     6361 gaaaaagagg ctgtaggtgt aaactttttt cgagccaaac ttatcaagca gccagccgcc
     6421 gggaatttgc atcagcaggt aggcccagcc aaaagcggag aagatgtaac ccatcgaaac
     6481 cgtacttaac tgcaactctt ttgccacttc ggtaccagca atagacagcg ttgcacaatc
     6541 ggcgtagtta acggcggtaa caataaaaat aatcagtaat attaaatagc gggtatgcac
     6601 gcctttcttt tttacgtcaa cggtgt
//
LOCUS       DADLFA010000196         6590 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000196 DADLFA010000000
VERSION     DADLFA010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6590
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.198"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            18..173
                     /locus_tag="JQH65_003545"
                     /pseudo
     CDS             18..173
                     /locus_tag="JQH65_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
     gene            166..1947
                     /locus_tag="JQH65_003546"
     CDS             166..1947
                     /locus_tag="JQH65_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HAY6140830.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKMVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            2128..2466
                     /gene="glnK"
                     /locus_tag="JQH65_003547"
     CDS             2128..2466
                     /gene="glnK"
                     /locus_tag="JQH65_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAY6140831.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            2496..3782
                     /gene="amtB"
                     /locus_tag="JQH65_003548"
     CDS             2496..3782
                     /gene="amtB"
                     /locus_tag="JQH65_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HAY6140832.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLMQVTVTFALVCILWVVYGYSLAFGEGNNFFGNVNW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQELEGLDVNSHGENAYNA"
     gene            complement(3831..4691)
                     /gene="tesB"
                     /locus_tag="JQH65_003549"
     CDS             complement(3831..4691)
                     /gene="tesB"
                     /locus_tag="JQH65_003549"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HAY6140833.1"
                     /translation="MSQALKNLLALLNLEKIEAGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            4909..5481
                     /locus_tag="JQH65_003550"
     CDS             4909..5481
                     /locus_tag="JQH65_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY6140834.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(5512..5823)
                     /locus_tag="JQH65_003551"
     CDS             complement(5512..5823)
                     /locus_tag="JQH65_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HAY6140835.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            5986..6082
                     /gene="ffs"
                     /locus_tag="JQH65_003552"
     ncRNA           5986..6082
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="JQH65_003552"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            6202..6555
                     /locus_tag="JQH65_003553"
     CDS             6202..6555
                     /locus_tag="JQH65_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HAY6140836.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
BASE COUNT         1443 a         1637 c         1916 g         1594 t
ORIGIN      
        1 gggatgcgtt catataaatt attgtgatgc agcacggaca tatcgcccag cgtggcaatc
       61 atgatgagtt ggcgcaacaa agcggctggt atcgcgatat gtatcgctat caacaactgg
      121 aggcggcgct cgacgacgct ccggaaattc gcgaggaggc cgtcgatgcg tagttttagc
      181 caactgtggc cgactctcaa gcgtctgtta gcgtacggtt cgccgtggcg taaaccgctg
      241 gggattgcgg tcctgatgat gtgggttgcg gcagcggcag aagtcagtgg gccgctgctt
      301 atcagctatt ttatcgacaa tatggtagcg aaaaataacc tgccgttgaa aatggttgca
      361 gggctggctg cggcgtatgt tgggctacaa ctgtttgccg ccgggctaca ttacgcgcag
      421 tcgctgctgt ttaatcgggc ggcagtaggc gtagtgcaac agttgcgtac cgacgtgatg
      481 gatgccgcat tgcgccagcc attaagcgag tttgataccc aacccgtcgg gcaggtgatt
      541 tcccgcgtca ctaatgacac tgaagtgatc cgcgatctct acgttaccgt agtggcaact
      601 gtcctgcgta gcgccgcact ggtgggcgcg atgctggtgg cgatgttcag cctcgactgg
      661 cgaatggcgc tggtggcgat aatgattttc ccggtggtgc tggtggtaat ggtgatatac
      721 cagcgttaca gcacgccgat tgtccgtcgt gtgcgcgcct atctggcgga tatcaacgac
      781 ggctttaacg aaatcatcaa tggcatgagc gttatccagc agttccgtca gcaggcgcga
      841 tttggcgaac gtatggggga ggccagtcgt tcacactata tggccaggat gcaaaccctg
      901 cgcctcgacg gttttctgct gcgtccgctg ctgagtctgt tttcatcgct cattctttgt
      961 ggcttgttga tgctgtttgg cttctccgct agcggcacca ttgaagtagg cgtgctgtat
     1021 gcgtttatca gctatcttgg gcgacttaac gaacccttaa tcgaactgac cacgcaacag
     1081 gcgatgctgc aacaggctgt tgttgctggt gagcgcgtgt ttgaactgat ggacggaccg
     1141 cgccagcaat atggcaatga tgatcgcccg ttacagagtg gcaccatcga agtcgataac
     1201 gtgtcatttg cttatcgcga tgacaatctg gtgctaaaga acattaatct ctctgtgcct
     1261 tcgcgtaact ttgtggcact ggtcgggcat accggcagtg gcaaaagcac cctcgccagt
     1321 ttattgatgg gctattaccc gctaacggaa ggtgagattc gccttgatgg tcgtccatta
     1381 agttcgctaa gtcacagcgc gctgcgccag ggcgtggcaa tggtgcagca agatccggtg
     1441 gtgctggcgg ataccttcct cgccaacgtg acgctggggc gtgatatctc cgaagaacgc
     1501 gtctggcagg cgctggaaac cgtgcaactg gcggagctgg cgcgcagcat gagtgatggt
     1561 atttatacgc cgctgggcga gcaggggaat aatctctccg tcgggcaaaa gcaactgctg
     1621 gcactggcac gcgtgttggt ggagacgccg caaatcctga tccttgatga ggcaaccgcc
     1681 agcattgact ccggtactga acaggcgatt caacatgctc tggcggctgt gcgtgaacat
     1741 accacgctgg tggtgattgc tcaccgctta tcgaccattg ttgatgccga caccattctg
     1801 gtgcttcatc gtgggcaagc cgtggagcag ggcactcacc agcaactgct ggcggcccag
     1861 ggacgctact ggcagatgta tcaactgcaa cttgcgggcg aagagctggc agccagcgtg
     1921 cgtgaagaag aatcattgag cgcctgaata gcgcaatatt tcatcgttgg tgcaaaaatg
     1981 taacgcactg tgcactgtca tagtgcgttt tcattttcaa tcttcttaac ttcctgctct
     2041 ctttctcgtt tttcatttct ggcacaccgc ttgcaatacc ttcttcgtgt agcagaacca
     2101 ttaccgaatt ctgactggag gggatctatg aagctggtga ccgtgataat caaaccattc
     2161 aagctggaag acgttcgtga agcgttatct tccattggta ttcagggcct gaccgtcacc
     2221 gaagtgaaag gtttcgggcg tcagaaaggg catgccgagc tgtaccgggg ggcggaatac
     2281 agcgtcaact tcctgccaaa agtaaaaatt gatgtggcaa ttgctgatga ccaactcgat
     2341 gaagtgatcg atatcgtcag taaggcggct tacaccggaa aaattggcga cggcaaaatc
     2401 ttcgtcgctg aattgcaacg cgtcattcgt attcgtaccg gcgaagccga cgaagcggcg
     2461 ctgtaatctc tggcacacag caacaggaac gaaaaatgaa gatagcgacg ataaaaactg
     2521 ggcttgcttc actggcgatg ctgccgggac tggtaatggc tgcacctgcg gtggccgata
     2581 aagccgacaa tgcgtttatg atgatttgta ctgcgctggt gctgtttatg actattccgg
     2641 ggattgccct gttttacggt gggttgattc gcggcaaaaa cgtgctgtcg atgctgatgc
     2701 aggtgacggt gacgtttgca ctggtctgta ttctctgggt ggtttacggt tactcgctgg
     2761 cgtttggtga gggtaacaac ttcttcggca acgttaactg gttgatgctg aaaaacatcg
     2821 aactgacggc ggtgatgggc agcatttatc agtatatcca cgtagcgttt cagggatcgt
     2881 ttgcctgcat taccgtaggc ttgatagttg gggcgctggc ggaacgaatc cgcttctcag
     2941 ctgtgttgat tttcgtggtg gtatggctga cgctctctta cattccgatt gcgcatatgg
     3001 tgtggggcgg cggtttgctg gcttctcacg gtgcactgga tttcgcgggt ggcacagtgg
     3061 tgcacattaa cgccgcaatc gccggtctgg tgggcgcgta tctgataggc aaacgcgtgg
     3121 gcttcggtaa agaggcgttt aaaccgcaca acctgccgat ggtcttcacc gggactgcca
     3181 ttctctatat cggttggttt ggctttaacg ccgggtcagc gggcacggcg aatgaaatcg
     3241 cggcactggc atttgtgaat actgtggtcg caacggcggc ggcaattctt ggctggatct
     3301 tcggtgaatg ggcgctgcgt ggtaagcctt cactgctggg ggcgtgttct ggcgcgattg
     3361 ccggtctggt cggcgtgacg ccagcgtgcg gctacattgg ggttggcggc gcgttgatta
     3421 tcggcgtggt agctggtctg gcgggcttgt ggggcgttac catgctcaaa cgcttgctgc
     3481 gggtggatga tccctgcgat gtcttcggtg tgcacggcgt ttgtggcatt gtcggctgta
     3541 tcatgaccgg gatttttgcc gccagctcgc tgggcggcgt gggcttcgct gaaggtgtga
     3601 cgatgggtca tcagttgctg gtacagctgg aaagcatcgc cattacgatc gtctggtccg
     3661 gtgtggtggc atttattggc tacaaattgg cggatctgac ggttggtctg cgtgtaccgg
     3721 aagagcagga gctagaaggg ctggatgtca acagtcacgg cgagaatgcc tataacgcgt
     3781 aacaagcact gcaaaaaaca gccggacggt tttcacctcc ggctattttt ttaattgtga
     3841 ttacgcatca ccccttcctg aacggtcgag gcaaccagta cgccgtcctg ggtataaaac
     3901 tcaccgcgca caaagccacg tgcgctggac gccgaggtgc tctccacgct atacagcagc
     3961 cattcattca aattaaacgg gcgatggaac cacatggaat ggtcaatggt ggcaatctga
     4021 atccccggtt cgagaaaacc gatgccgtgc ggctgtagag ctaccggcag gaagttaaga
     4081 tcagaagcgt aaccgagcag atactgatga acgcgcaggt catccggcac gctaccattt
     4141 gcgcggatcc acacctgacg atgtggttct gcgacgtgac ctttcagtgg gttatgaaac
     4201 tccaccggac ggacttccag cggacgatcg cagatgaatt tatctttaag cactggcggc
     4261 agcaggtgcg ccagcgattg ggcgatttgc gtttccgaag gcaggccatc aggcgctggc
     4321 gcggacggca ttgttttttg atgttcgaaa cccgcttctg gtgcctggaa agaggccgtc
     4381 atataaaaaa tcggtttgcc gttttgaata gcagcaaccc ggcgggcgct gaagctgtta
     4441 ccatcacgca gcgtttcgac atcataaata atcggtttct tactatcgcc tgggcgaaga
     4501 aagtagctgt gaaacgaatg taccagccgc tcttccggga cggtctcttt tgcagcatac
     4561 aaggcctgac ccacgacctg gccgccaaac acctggcgta aacctaaatc ttcactctgg
     4621 ccgcgaaaga gtcctgcctc aattttttcc agatttaaca atgccagtaa attttttagc
     4681 gcctgactca tataactctc cagtaacaaa gctgccgcag caagccaaag tgagttgagt
     4741 ataacgcaaa tttgccactg gtccgatggg tgcaataatc tgaattacgg gctaattaca
     4801 ggcagaaatg cgtgatgtgt gccacactta ttgacgttac tattttgtta accactcttc
     4861 cggcgaggaa agttagcccg ctggtgcatt gataataagg agaaatgaat gaaactcgtg
     4921 cacatggcca gtggtttagc ggttgcgatt gcgttggcgg cttgcgcaga taaaagcgcg
     4981 gatattcaga cgccagcccc ggctgcaaat acgtctattt cagcaacaca acaacctgct
     5041 atccagcaac cgaatgtctc cggtaccgtc tggatccggc agaaagtcgc actgccgcct
     5101 gatgctgtgc tgaccgtgac actttctgac gcgtcgttag ccgatgcacc gtcaaaagtg
     5161 ctggcgcaga aagcggtgcg taccgaaggt aaacagtcac cattcagctt tgttctgcca
     5221 tttaacccgg cagatgttca gccgaacgcg cgtattctgt tgagtgcggc gattaccgtg
     5281 aatgacaaac tggtatttat taccgatacc gttcagccgg tgatcaacaa gggcggaact
     5341 aaagccgacc tgacattggt gccggtgcag caaaccgccg tgccggttca ggccagcggt
     5401 ggtgcaacga ctaccgtacc ttcgacttca ccaactcagg tgaatccgtc ttcggcagtt
     5461 ccagctccta cgcaatatta agcgacgtta accctctccg cccggagagg gttagtagtt
     5521 ccagcgataa cgctgcaagt cgatttgccc gcttcccgat accatcacac cttctgccag
     5581 taatgcctgt cgctgacgct gtaaatccgg tccggttagc gaaattgtgc cgtggcgatt
     5641 aaccacccgg tgccagggta aggtgctgcc ttcagggaga cgctttaaca caccgcccac
     5701 ctggcgcgcg gcgcggggcg atcccgccag tttcgccaca tcaccgtaag tggtgacata
     5761 gccttcggga atagcggcga cgatttgcca gacgcgttgg ggaaatgaat cttctttttc
     5821 catcttttct tcctgaggta atttttcagc ataatctgga aaaacgcccg agtgaagtcg
     5881 cattgcgcaa gaaaccagca tctggcacgc gatgggttgc aattagccgg ggcagcagtg
     5941 ataatgtgcc tgcgcgttgg ttctcaacgc tctcaatggg ggctctgttg gttctcccgc
     6001 aacgctactc tgtttaccag gtcaggtccg gaaggaagca gccaaggcag atgacgcgtg
     6061 tgccgggatg tagctggcag ggcccccacc catttctgcc tcccaccgtt tcgtcaaaaa
     6121 aacccaacat ggctaaactt taaccacaac tgacgtcgca agaattgtct ggctgcgcag
     6181 tacgcttcgg aggtatgtct gatgaagtat gttgatggtt ttgtggttgc cgttcctgcc
     6241 gataaaaagg atgcctatcg ggaaatggcc gctaaggctg cgccattgtt taaagagttt
     6301 ggcgcgcttc gtattgtcga atgctgggcc agcgatgtac cggatggcaa agtgaccgat
     6361 tttcgtatgg cggtgaaagc ggaagagaat gaagaggttg tctttagctg gattgaatac
     6421 ccttcaaaag aggtccgcga cgctgctaat caaaagatga tgtcggaccc acggatgaaa
     6481 gagttcggcg agtccatgcc gtttgacggc aagcgaatga tctatggcgg attcgagtca
     6541 atcatcgacg aataggtagc gtgacgggct gtgcggcgtg ccgtcagccc
//
LOCUS       DADLFA010000197         6440 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000197 DADLFA010000000
VERSION     DADLFA010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6440
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.199"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..348)
                     /locus_tag="JQH65_003554"
     CDS             complement(<1..348)
                     /locus_tag="JQH65_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HAY6140837.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSDEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLA"
     gene            456..734
                     /locus_tag="JQH65_003555"
     CDS             456..734
                     /locus_tag="JQH65_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HAY6140838.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSANVSAPAQST"
     gene            960..3370
                     /locus_tag="JQH65_003556"
                     /pseudo
     CDS             960..3370
                     /locus_tag="JQH65_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
     gene            3431..5854
                     /locus_tag="JQH65_003557"
     CDS             3431..5854
                     /locus_tag="JQH65_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HAY6140839.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKCGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMLDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            5865..>6440
                     /gene="dmsB"
                     /locus_tag="JQH65_003558"
     CDS             5865..>6440
                     /gene="dmsB"
                     /locus_tag="JQH65_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HAY6140840.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELGCKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICIESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPT"
BASE COUNT         1740 a         1646 c         1582 g         1472 t
ORIGIN      
        1 tgctagctgg cattttccct gtaaacgtac tctcttctcc agcgtctcgg catcacggta
       61 ataattggcg tccagtacca gagcgaccac ggtattattt tccagatcca gtaattcgag
      121 tgaatcaaaa cagccttctt ccgacaaagt cccagaaaca cgtttcgtca cttcaccttg
      181 ctcatccatt aatgtctgag tgaaatcttt taccggacca cgcagcggat caaaatcgaa
      241 ttcatcagag aaactggcca tctcaggggt aaatgaaagt gttacctctg tgcggtcaca
      301 tcctgtgagg aatatcgcga gtaagcatgg tagtaatttg tatttcacaa cagtcaccag
      361 agagtagaga tgattctcaa tcatagtagc aaatacagta ctttacacgt taaatgctat
      421 gcttaaagaa gttatcttcg cgtaaggagc ttatgatgaa actctcaacc tgctgtgccg
      481 cgcttctgct cgccctcgcc tcacccgcgg tactcgccgc gccgggatcc tgtgagcgca
      541 tacagagcga tatatcacag cgcattatca ataatggcgt acccgaaagc agcttcacat
      601 taagtattgt acccaatgac caggttgatc agcctgattc ccaggtcgtc gggcattgtg
      661 ctaatgatac gcataaaatt ctctacaccc gcaccaccag cgctaacgtc tctgctcccg
      721 cgcagtctac ctaggatggc gcgcctgccg aaccgcagta atacattatc gactgaacgc
      781 cggatatgac acatccggcg atttgaacat acaacataat cccaccttat tactcatacc
      841 cttctattga tgtggattaa taattcttaa cccaaaatgg gtagaccccc tctattgtta
      901 gcgcgctaaa tattcaatac ataaacattt atataacgat aaagaacagg gagtgagtta
      961 tgtccaaaaa tgaccgaatg gtgggcatca gccgcagaac actcgttaaa tcaaccgcga
     1021 taggttctct ggcgctggct gcaggcggtt tttctttacc gttcaccctg cgcagtgcag
     1081 cggcaacggt acaacaggcc agcgaaaaag tgatctgggg tgcctgttcc gtcaactgtg
     1141 gtagccgctg tgcacttcgt ctacatgtta aagataatga agtgacctgg gtggaaactg
     1201 acaataccgg cagcgatgag tacggcaacc atcaggtacg cgcctgtttg cgcggtcgct
     1261 ccatccgccg gcgtattaat catcccgatc gcttgaatta cccaatgaaa cgcgtgggta
     1321 aacgcggtga aggcaaattc gaacggatta gctgggatga agccctggat actatcgcca
     1381 gtagcctgaa aaaaaccgtc gaacaatatg gcaatgaggc tgtatatatt cagtactctt
     1441 cggggattgt tggcggcaat atgacccgct cttcgccatc agcctcggcg gtcaaacgcc
     1501 tgatgaactg ctacggcggt tcactcaatc agtatggctc ctacagcacc gcacaaattt
     1561 cctgtgccat gccctacacc tacggcagta atgatggcaa cagcaccacg gatattgaaa
     1621 acagcaagct ggtcgtgatg tttggtaaca acccggcaga aacccgcatg agcggcggtg
     1681 gcatcactta tcttcttgaa aaagcgcgcg agaaatcgaa cgccaaaatg attgttatcg
     1741 atccgcgata taccgatacg gctgctggtc gtgaagatga atggcttcct attcgcccgg
     1801 gcaccgatgc cgcgctggtt gcgggtattg cctgggtatt gattaacgaa aatctcgttg
     1861 atcaaccttt tctcgataaa tactgcgtcg gctatgacga aaaaacctta cccgcagatg
     1921 caccaaaaaa tggtcactat aaagcctata ttcttggtga aggtgatgat aatacagcga
     1981 aaacgccgca gtgggcttcg caaattaccg gtatcccggt ggaccgtatc atcaaactgg
     2041 cgcgtgaaat tggcacagca aaacccgctt atatctgcca gggctggggg ccgcaacgcc
     2101 aggcaaacgg cgaactgact gcacgcgcta ttgctatgtt gcctattttg acgggcaatg
     2161 tcggcatcag cggcggaaat agtggcgcgc gtgaatcgac ctataccatt accatagaac
     2221 gcctgccggt gctggataat ccggtcaaaa cgtcaatctc ctgcttcagc tggacagatg
     2281 ctatcgatca tggtccgcaa atgacggcaa tccgcgacgg cgtccgcggc aaagataaac
     2341 tggatgtgcc cattaagttc atctggaact acgcgggaaa taccctcgtt aatcagcatt
     2401 ctgacatcaa caaaacgcat gaaattctgc aggacgaatc gaaatgcgaa atgattgtgg
     2461 tcatcgaaaa ctttatgacc tcatcggcaa actgccagac ctgatgaccg ttgagcagga
     2521 agatattatt cctaacgact acgccggtaa catgggatat ctcattttcc tccagcctgt
     2581 caccagcgaa aaattcgaac gcaaaccgat ttactggatc ctgagtgaag tcgcgaaacg
     2641 tcttggacct gacgtctatc aaaagttcac agaaggtcgc acgcaggaac aatggttaca
     2701 acatctgtac gccaaaatgc ttgccaaaga tccggcgtta ccgtcttacg acgaactgaa
     2761 aaaaatgggt atctataagc gtaaagatcc caatggccat tttgtcgcct acaaagcatt
     2821 tcgtgacgac cccgaggcaa atccacttaa aacgccttcc ggtaagattg aaatttattc
     2881 cagcaggctg gcggaaattg cccgtacctg ggaactggaa aaagatgaag tgataagccc
     2941 attgccagtt tatgcctcaa cctttgaagg ctggaactcc cctgaacgta gaaccttccc
     3001 actgcaactg tttggtttcc attacaaatc ccgtactcac tcgacctacg gcaatattga
     3061 tctcctgaag gctgcctgcc gtcaggaggt gtggatcaac cctatagatg cgcagaaacg
     3121 tgggattgcc aacggcgata tggtgcgggt gtttaaccat cgtggcgaag ttcggctacc
     3181 agccaaagta acaccacgta ttctccctgg agttagcgct atgggccagg gagcctggca
     3241 cgaggccaat atgtctggcg ataaaatcga ccatggcggc tgtgtgaata cgctaaccac
     3301 tctgcgccct tcaccactgg cgaagggaaa cccgcagcac actaatctgg tcgagatcga
     3361 aaaaatataa cccacgacaa ccataaattc tggcatgaca ttttgtttga aaagcaataa
     3421 gtgagtaatg atgaaaatcc ataccacaga ggcgctcatg aaggctgaaa tcagccgtag
     3481 aagtctgatg aaaacctccg cacttggcag tcttgcgctg gcaagcagtg ctttcactct
     3541 gccattttcc caaatggtcc gggcggcaga ggctccggta gaagagaaag cggtctggag
     3601 ttcctgcacc gttaactgcg ggagccgctg tctgttacgt ttgcatgtga aagatgacac
     3661 cgtgtactgg gtggagtctg atacgacagg tgacgacgtc tacggtaatc atcaggttcg
     3721 agcgtgttta cgcgggcgct ctatccgccg acggatgaat catcctgata ggttgaaata
     3781 tcccatgaag cgcgtcggca agtgcggtga aggtaaattt gaacggataa gttgggacga
     3841 agccctggat accatcagtg ataatcttcg gcggatcctg aaagattacg gcaatgaggc
     3901 tgtacatgtc ctgtacggaa caggcgtaga tggcggaaac atcaccaact caaacgtccc
     3961 gtaccgtctg atgaactctt gcggtggttt tctcagtcgc tatggcagct acagtaccgc
     4021 acagatcagt gccgcaatga gttatatgtt cggtgccaat gatggcaaca gcccggatga
     4081 tatcgccaat acgaaactgg tcgttatgtt cggaaataac ccggcagaaa cgcggatgag
     4141 cggcggtggt gtcacttact acgtcgagca agcccgcgaa cgttcaaacg cacgcatgat
     4201 cgtcatcgat ccacgttata acgacactgc tgccgggcgt gaagatgaat ggctgcctat
     4261 tcgccctggc accgatggcg cactggcctg tgcgattgcc tgggtactga ttactgaaaa
     4321 catggtcgat cagccatttc tcgacaaata ttgtgttggt tacgatgaaa aaacgctgcc
     4381 cgccaacgca ccacgtaacg cgcattataa agcctatatt ctgggcgaag ggcctgacgg
     4441 catagctaaa acgccggaat gggcagcaaa aatcaccagc atcccggcag aaaaaattat
     4501 ccagttggcg cgagagatcg gttcagcaaa acctgcttat atttgtcagg gttgggggcc
     4561 acaacgacat tccaacggcg agcaaacgtc ccgcgctatt gccatgcttt ccgttctcac
     4621 cggtaacgtt ggcataaacg gcggcaactc aggtgtacgc gaaggtagct gggatctggg
     4681 ggtagaatgg ttcccgatgc ttgagaatcc tgttaaaacg cagatttccg tctttacatg
     4741 gacagatgcc atcgaccatg gtacggaaat gaccgcgacc cgtgatggtg ttcgtggaaa
     4801 agaaaaactg gatgtcccca tcaagttttt atggtgctac gccagtaaca cattgatcaa
     4861 tcaacatggc gacatcaatc acacccatga ggtgcttcag gatgacagca agtgcgagat
     4921 gattgttggc attgaccact tcatgacggc ctcggctaag tattgcgata tcctgttgcc
     4981 cgacctgatg ccaacagagc aagaagacct tatctctcat gaatctgcag ggaatatggg
     5041 ctatgtgatc ctcgcccaac ccgcaacctc agcaaaattt gaacgcaaac ccatctactg
     5101 gatgctgagt gaagtcgcca aacgcttagg accagacgtt tatcaaacct ttactgaagg
     5161 tcgcagtcag catgaatgga tcaaatatct ccatgcgaaa acgaaggaac gtaaccctga
     5221 gatgctcgac tacgaggaga tgaaaacgac cgggatcttt aagaaaaaat gcccggaaga
     5281 acactacgtc gctttccgcg cattccgtga agatccacag gcaaacccgt tgaaaacacc
     5341 ttcggggaaa atcgaaattt attctgaacg actggcgaag attgcagata cctgggaatt
     5401 gaaaaaagat gaaattattc atccccttcc tgcgtatacc ccaggttttg atggctggga
     5461 cgatcccctg cggaaaacct atccactgca gttaacgggc ttccactaca aagcgcgtac
     5521 ccactccagc tacggcaata ttgatgtgtt acagcaggcc tgcccacaag aggtgtggat
     5581 caaccccatt gatgctcagg cacgcggtat ccgtcatggc gataccgtgc gggtatttaa
     5641 caataatgga gaaatgctga ttgccgcaaa agtgactccg cgtattctgc ctggcgttac
     5701 cgccatcggg caaggtgcgt ggcttaaagc ggatatgttt ggtgaccggg tcgatcacgg
     5761 cggcagtatc aatattctga cctctcaccg cccttcaccg ctggcaaagg gaaacccgtc
     5821 gcacagcaat cttgtccaga tcgaaaaggt ttaaggagta gcccatgacc acacaatatg
     5881 gattttttat cgattccagc cgctgtactg gctgtaaaac ctgcgaactg gggtgcaaag
     5941 attttaaaga tcttggcccg gaagtcagtt tccgccgtat ttatgaatat gctggcggcg
     6001 actggcagga agataatggc atctggcacc agaacgtgtt cgcttattat ctctccatct
     6061 cctgtaacca ttgcgatgac cccgcatgta caaaagtctg ccccagcggc gcaatgcata
     6121 agcgtgaaga tggctttgtg gtggttgatg aagatgtttg tatcggctgc cgctactgcc
     6181 acatggcttg tccgtacggc gcaccacagt acaatgctga aaaagggcac atgacgaagt
     6241 gcgatggttg ttattcgcgc gtcgccgagg ggaaacaacc catatgcatc gaatcctgcc
     6301 cgctgcgtgc acttgagttc ggtccaattg aagagctgcg ccagaagcac ggcactctgg
     6361 ctgccgtcgc gccactgccg cgcgcgcatt tcacaaaacc caatatcgtt atcaaaccta
     6421 acgccaacag ccgcccgacc
//
LOCUS       DADLFA010000198         6386 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000198 DADLFA010000000
VERSION     DADLFA010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6386
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.200"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..202)
                     /locus_tag="JQH65_003559"
     CDS             complement(<1..202)
                     /locus_tag="JQH65_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HAY6140841.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFR"
     gene            681..2458
                     /locus_tag="JQH65_003560"
                     /pseudo
     CDS             681..2458
                     /locus_tag="JQH65_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
     gene            complement(2498..2977)
                     /gene="xdhC"
                     /locus_tag="JQH65_003561"
     CDS             complement(2498..2977)
                     /gene="xdhC"
                     /locus_tag="JQH65_003561"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HAY6140842.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(2974..3852)
                     /gene="xdhB"
                     /locus_tag="JQH65_003562"
     CDS             complement(2974..3852)
                     /gene="xdhB"
                     /locus_tag="JQH65_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HAY6140843.1"
                     /translation="MFDFASYHRAATLADAINLLANNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAAMSIAG
                     LQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(3863..6160)
                     /gene="xdhA"
                     /locus_tag="JQH65_003563"
     CDS             complement(3863..6160)
                     /gene="xdhA"
                     /locus_tag="JQH65_003563"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HAY6140844.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAIAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGTFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
BASE COUNT         1594 a         1511 c         1614 g         1667 t
ORIGIN      
        1 cgcggaatac ggaaatacct aatccactat taaagacttt ggttgagatg ttttcagcac
       61 gcagtgcttt taatgctgcg gcaacgtcca gtacttgttt cagttcatct ggcgtctgtt
      121 cccacgttaa caaaaaatct ttctcgtgaa ggtgagaggt cagcgaattg atatccttaa
      181 tcagctcatt aacagttttc atcataaagt cccggtagtg gaatgacagg caaagtggtt
      241 tatttcactg tgacttgcca aagtgattgg tgttttcaca gctatacctg ggactaacaa
      301 taagtgtgcc agaattatcc gcgcggctgc aaaagactaa tttgacggat aatgtgtggg
      361 gttgatcaca aattgatgcc agcttctggt tgtttgtaaa atacgaaaac gtttggcttt
      421 ttttatcgcg ttatttattt gctgctaagt atcagaaata agataaaggg gcaggctatc
      481 tctcttcagt ttgataaact caccgccgta attctcatat tgatatagaa atgtgatatc
      541 tcttccataa tttcattaga aaatgataaa aatcttatgt ttttatcaaa gccaatttcg
      601 cctgagttaa tatcatcatg atctgtgaac catcaacgtc ttcaatgatc gacttttaag
      661 taagtctgga aggtaaagct atggagcttg ctactacgca gtcagtattg atgcaaattc
      721 aaccgacaat tcagcgtttt gccagaatgc ttgccagcgt tttgcagctt gaggttgaga
      781 tcgttgatga aaacttgtgt cgcgtcgccg gaacgggcgc gtatgggaag tttcttggtc
      841 gccagttgag cggcaactca cgcctgctcc gccacgtcct ggaaacgaaa actgaaaaag
      901 ttgtgacaca gtctcgcttc gatccccttt gcgaaggttg cgatagtaaa gaaaattgcc
      961 gtgaaaaagc atttctgggt acgcctgtca ttttacagga tcgttgtgtt ggggtgataa
     1021 gtttgattgc cgttacccac gagcaacaag agcatatcag tgataattta cgcgaatttt
     1081 ctgattatgt tcgccatata tccaccattt ttgtttcgaa acttctggag gatcaggggc
     1141 caggagataa catcagtaaa atattcgcga ccatgatcga taatatggat cagggcgtat
     1201 tagttgttga tgatgaaagt cgggttcagt ttgttaatca gactgcctta aaaacacttg
     1261 gtgttgtaca aaataatatt attgggaaac ctatccgttt cagaccatta acatttgaga
     1321 gtaattttac tcatggacat atgcagcata ttgtttcgtg ggacgataaa agtgaattaa
     1381 tcattggtca attgcataac attcagggcc gacaattatt tttaatggca tttcaccaat
     1441 cgcataccag tttttctgta gcaaatgcac ctgatgaacc acatattgaa caattggttg
     1501 gcgagtgccg tgttatgcgg caattaaaac gactcattag ccgtattgca cccagcccat
     1561 ccagcgttat ggtggttggt gaaagcggca cgggtaaaga agtcgtcgcc cgagcaatcc
     1621 ataagttgag cggaagacgg aataaaccct ttattgctat caactgtgcc gcgattccgg
     1681 agcagcttct ggaaagcgaa ctgttcggtt atgttaaagg cgcatttact ggcgcttctg
     1741 ccaacggtaa aacagggttg attcaggcgg cgaatacggg cacgctgttt ctcgatgaaa
     1801 taggtgatat gccattaatg ttgcaggcta aattactgcg cgctattgag gcgcgtgaaa
     1861 ttctgccgat tggtgccagt agcccaatac aagtcgacat tcgcatcatt tctgcaacta
     1921 atcagaattt ggcccagttc attgccgaag gtaaattccg cgaagatctc ttctaccgac
     1981 ttaatgttat cccgataact ctgccaccgc tgcgtgaacg tcaggaagat attgaacatt
     2041 ggtgcattac tttttacatc tgcatacccg tcgtctggga tcggtttatc ctggcattgc
     2101 tcccgatgtc gtcgaaatat tgcgtaagca tcgttggccc ggaaacctgc gcgagttaag
     2161 caatttgatg gaatatctgg ttaacgtagt tccttcaggt gaagttatcg acagcacgct
     2221 attgccgcca aatctgctga ataatggcac aacggagcaa agtgatgtaa cagaggtcag
     2281 tgaggcgcac ctgtcactcg atgatgcggg cggcacggcg ctggaggaga tggaaaagca
     2341 aatgatccgc gaggcgcttt cacgtcataa cagcaagaag caagttgctg atgaactggg
     2401 catcggcatt gctacgctct atcgcaagat taagaaatat gagttgttaa acacataacc
     2461 tcagtgaatc cggcctgaat tcaggccgga tatcctttta cttcgttttc tcgcaatcca
     2521 gaactgtatt tacaatcatc tgataccccg tgcagcgaca aagatttccc gccagtccgc
     2581 gacgaatttc cgtaatggtt aatggcttct cgcgtggttt cgccagcatt gccgtggtag
     2641 ccataatcag gccaggcgta caaaacccgc actgcactgc gccggatttc gcataagcct
     2701 gctgaacatg agaaagtttt ccgcctttcg cttcaccttc cagcgtgcgg atctcttttc
     2761 cttcagccca ggcggcaagg tataagcaac tgtctattgc tgtgccgtcg accaacaccg
     2821 tacaggcacc acattcaccc acgcagcacc cttgtttgac acttagcagt ccttgttcgc
     2881 ggagtaattc cgagagcggc gtgcctggtg cggcgtgaag ctgaaaaggc atcccgttaa
     2941 tggtgcattc gatggtaatt gtttcgctgt gattcattgc aattttcccc ccgccgcggc
     3001 gacggcttcg ctaatcactt ttttggtcat cgtctggatg agatgcagac gaaactcttt
     3061 actggcccgc catgaagaac gcggggcgac atcttgcagg acagattcgc tgatagcttc
     3121 cagcgtttgc aggtttaatg gcgcattttg tgcagtctgt tcggcatgtt ggcagcgaat
     3181 cggcgttggc gcggcaacac caaatgccag gcgtaattcg ctgaaattgc cgttatccag
     3241 tcggcaatgt gcggcgcagc caatcgttga aatatccatt gcgtcgcgca tggcatattt
     3301 aaaatgcgcg ctgcccgcgt gttctttcgg ctgtggcgga aaatgaaagg cgacgaggat
     3361 ttcgtcatgc tcaagagaca ctttgcccgg cccggtgtga aagccattaa tcgggacgaa
     3421 acgaacaccg cgcggggagt ggatctccag tttcgcgtca taaattagcg ttggcgtggc
     3481 agaatctgcg ctggtggcac cgttgcaaat atttccaccg taggtagcga cgttacggat
     3541 ctgcagtcca gcaatggaca tggccgcagc acataacgcc gggagatgac gttgagttat
     3601 aggatcttct attagctggg taaatgtcgt tgcagagccg atacgtagcg agccatcttc
     3661 cgccagcgta attccccgca gctccgccag attatgaata tcaacaatat ggcgataacg
     3721 gtcattgtga tggtggagct gaatcagtac gtcagtgcca ccggcgagca gtttggcctg
     3781 cgggttgtta gccagcaggt tgatggcatc ggcaagggtt gctgcgcgat ggtaagaagc
     3841 aaaatcaaac atgatgttat cctcaaatca atcctgccag atggaactct tcatataacc
     3901 gttttggcgt cagcggcagt gtattgattg caacaccggt agccatcttc accgcgttac
     3961 gaatagcagc ggcaacagga attattggtg gctcacccag tgacttatgt ccgtatgcgg
     4021 attgcggctc attgatttcg acgaacgcgc tttccagttg tggcagatcc ggcatggtcg
     4081 gcattttgta atccagcaga ttggggttac ggaccacgcc gcttttagca tcgatgatca
     4141 tctcttcaaa tagcgcccag ccaatgccca ttcccattcc gccgtgtacc tgaccttctg
     4201 ccagcagtgg attaagaata tgccctgaat catgaacgtt gaggatgcgg ttgatggtga
     4261 ctttgcacag cgcaatatcg accgtcagat caacaaaggt acagccaaac gccggtgggt
     4321 tagtggtggt tttgatggag ctttcagcag agagctgccc gccgcgttca gggtggtaga
     4381 aagcgtccat cgccaaatct tttaacgaca ttaacggctc ttccggtcgt tcaaccagca
     4441 cgatatggcc ttttatcagg gtcagattca tcgctgactg atgtagcatg actgcggcgt
     4501 gagcgatgat tttctctttt aataatagtg ccgcactgcg cagcgcaggc gcggcaacat
     4561 agctctggcg tgaggcaaat gtgccgggat cgaacggcgt aacgtcggta tcttgagttg
     4621 aaataacgcg aacgtcgctg accggaaccc ccacggtttc tgccaccatt tgcgagaaga
     4681 cggtgtcggc accctgaccg atttccgtcg cgccgctttg cacgttgatg gttccatcct
     4741 gattcatcag aaggcgcgcg cctgctattt ctacgccgac aggccaggtg ttagaggtgt
     4801 agctaaaaca ggcgacgcca acgccgcggc gcaaattgcc ttgctggttc tggcattctg
     4861 cacggcgttt ttcccattca aagattttcc ggcctttttc aagacactcc ggcaaccctg
     4921 cgctgtaaat acgtttgccc gtgagcggat tagcatctcc ttcgcgggcg gcgttgcgta
     4981 aacgaatttc aacaggatca atacctaacg ctgtcgcggc gtcatcaagc atagactcaa
     5041 cggcaaatac gacttgtggc gcgccataac cacgcatcgc accagccgag gggaggttgg
     5101 tatagcaggt ctttgaactg taagcgtagg cacaacgagg ataaaggtaa gcgactttat
     5161 tcccccccgc agaagcgatg gagtgcccgt gagatgcata agcgccggtg ttagacagaa
     5221 catccagact ataacctttc aatgttccgt cgcggttcac gcccatttgc ccgtcaatgg
     5281 taaaagcgtg gcgggtacgg gttgcgagga aacactcttc acggctaagg gaaactttca
     5341 ccggaatgcc gccaagcttg ctggtcagga atgccgccat tggctcttcc agtacatcct
     5401 gtttattacc aaaaccgcca ccgacaaatg gtttgatgac tcgtacgcat gaccagggaa
     5461 tatccagcgc ctgaccaacc acgcggcgaa caatgtgcgg gatctgggtg ctggaaacga
     5521 tggtaattcg cgagtcatcc tccatccacg ccagcgatgt tacgctttcc atgtgacaat
     5581 gttgaataac gggggtctga tagtgcccct gtacctggta gtcggcggca tcgattgttt
     5641 gttggacatt acccgtcgac atcgtgcttt gtttcagtaa attgccaccg ttatggattg
     5701 gtgcagcgtc ttctgccagc gccgcttctg gcgtggtgat aacgggtaat tcttgccact
     5761 caatgctgac caattgcgcc gctttttctg ccgtgagttc atcgcgggcc acgacgatgg
     5821 caatggcgtc accatgatga cgaacatggc gagttagcag tgcgcgatcg gcggtatcgc
     5881 gcttgttttc gtcaagtgtc caggcatgcc cagctgtagc gaatggaata tcaggcacat
     5941 cttcccaggt aaaaatcgcc agtacgcccg gcaaacttct ggcttgttca tcattaatac
     6001 ttacggcata accatgtgcg atagggctac gtacatattt cgcataacac atgcccgcca
     6061 taacataatc gtcagtatat cgtgcccgcc cggtgacctt agcaatggca tcgacgcgca
     6121 tgcatgattc acccgtagcg gttgcttccc gcgcttccat agaaatcccc tcgatttgaa
     6181 catttggttc ttatggcaaa gatttagtct aagcaggcaa gatttacgcc agaaacgacg
     6241 ataagcgtta ggggaggtag gaagtgtgat gacgttaaaa aaacacagat atgatacgaa
     6301 acaggagtct gaataggggc agtgatacgt atcaatatga gtggcgataa aatcaatgtg
     6361 ataaattggg attattacgc atcagg
//
LOCUS       DADLFA010000199         6337 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000199 DADLFA010000000
VERSION     DADLFA010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6337
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.201"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            30..152
                     /locus_tag="JQH65_003564"
     CDS             30..152
                     /locus_tag="JQH65_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAY6140845.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDT"
     gene            168..1013
                     /locus_tag="JQH65_003565"
     CDS             168..1013
                     /locus_tag="JQH65_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAY6140846.1"
                     /translation="MRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDA
                     IILSAVSENGSSRTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLSN
                     AAADYFIANKIDQPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDK
                     AISVGEKLIISTPDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELE
                     NNQVLKAVVDISGKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATH
                     VDGLP"
     gene            1036..2547
                     /locus_tag="JQH65_003566"
     CDS             1036..2547
                     /locus_tag="JQH65_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAY6140847.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            2572..3570
                     /locus_tag="JQH65_003567"
     CDS             2572..3570
                     /locus_tag="JQH65_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY6140848.1"
                     /translation="MSASSLSLPLGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTELSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            3603..4697
                     /locus_tag="JQH65_003568"
     CDS             3603..4697
                     /locus_tag="JQH65_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAY6140849.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALRSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            4709..5581
                     /locus_tag="JQH65_003569"
     CDS             4709..5581
                     /locus_tag="JQH65_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HAY6140850.1"
                     /translation="MTIYTLSHGPLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(5629..6051)
                     /locus_tag="JQH65_003570"
     CDS             complement(5629..6051)
                     /locus_tag="JQH65_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HAY6140851.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIILGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(6148..>6337)
                     /locus_tag="JQH65_003571"
     CDS             complement(6148..>6337)
                     /locus_tag="JQH65_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenoxybenzoate dioxygenase"
                     /protein_id="HAY6140852.1"
                     /translation="WLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSG
                     KTFDAKLLIDENIALKSL"
BASE COUNT         1469 a         1514 c         1834 g         1520 t
ORIGIN      
        1 ttgttaataa aaaccctaca ggaggcacta tgcctaaaaa aatgagaaca acccgtaatc
       61 tattattaat ggctacgcta ttaggtagcg cactatttgc cagggctgcg gataaagaaa
      121 tgaccattgg tgcaatatac cttgatacct agggatatta cgctggagtg cgtcagggcg
      181 ttcaggatgc ggcaaaagat tcttcagtac aggtacagtt aattgaaact aacgcccagg
      241 gtgatatttc gaaagaaagt acctttgttg ataccctcgt ggcgcgtaat gtcgatgcca
      301 ttattttatc ggcagtgtct gaaaatggca gtagccgtac cgttcgtcgc gccagtgaag
      361 cgggtattcc ggtgatttgc tacaacacct gtattaatca aaagggtgtc gataaatatg
      421 tctcggcgta tctggtcggc gatccactgg aatttggtaa aaaactgagt aacgctgccg
      481 ccgattattt tattgccaat aaaattgacc agccgaaaat tgccgtcatc aattgcgaag
      541 cctttgaagt ttgtgtgcag cgacgtaaag gatttgaaga agtattaaaa tcccgcgttc
      601 ccggcgcgca aattgtcgct aatcaggaag ggactgtttt agataaagcg atttccgttg
      661 gtgaaaaact gattatctcc acgccggatc tcaacgccat tatgggggag tcgggcggtg
      721 cgacactcgg cgcggtaaaa gcggtacgta atcaaaatca ggccggaaaa attgctgttt
      781 tcggttcgga tatgacaacc gaaattgctc aggagctgga aaacaatcag gtgctgaaag
      841 cggtagtgga tatttccggt aagaaaatgg gcaatgctgt tttcgcgcaa acattgaagg
      901 ttatcaataa acaagccgac ggtgaaaaag tgattcaggt gccaatcgat ctctatacca
      961 aaacggaaga tggtaaacag tggctggcaa cgcacgttga tggtctgccc taatcgctga
     1021 ttcgcagagg tccctatgtt cacggcaaca gaggcagtcc cggtagcaaa agtggtggca
     1081 ggaaataagc gttaccccgg cgtcgttgcg ctggataacg ttaacttcac gctcaataaa
     1141 ggcgaagttc gtgcgctgtt aggtaaaaac ggcgcgggca aatcgactct cattcgaatg
     1201 cttaccggca gcgaacgccc ggatagcggt gatatctgga ttggcgagac gcgactggaa
     1261 ggtgacgaag ctacgttgac tcgccgtgcc gctgaactgg gggttcgtgc ggtttatcag
     1321 gaattaagtc tggtggaagg gctgacggtg gcagaaaacc tctgccttgg tcaatggcct
     1381 cgccgcaacg gcatgattga ttacctgcaa atggcgcagg atgcccaacg ttgcttacag
     1441 gcgctgggcg ttgacgttag tcctgaacaa cttgtttcaa cgctaagccc ggcgcaaaag
     1501 caactggtgg aaattgcacg ggtgatgaag ggcgagccgc gcgtggtcat tcttgatgaa
     1561 cccaccagct cgcttgccag tgcagaagtt gaactggtga tcagtgcggt gaaaaagatg
     1621 tcggcactgg gcgtggcggt gatttatgtc agccaccgga tggaagaaat ccgccgcatt
     1681 gcctcctgtg ccaccgttat gcgcgatggt caggtggcgg gcgatgtgat gctcgaaaac
     1741 acctccacgc atcatattgt gtcgctaatg ctagggcgtg atcacgttga tattgcgccg
     1801 gttgcccctc aggaaattgt ggatcaggcc gtactggaag tccgtgcgtt acgccataag
     1861 cccaagctgg aggatatcag ctttacgtta cgtcgcggcg aagtgcttgg cattgctggc
     1921 ctgctggggg cagggcgcag tgaattgctg aaggcgattg ttgggctgga gacgtatgaa
     1981 cagggcgaaa ttgttatcaa cggcgagaaa atcacgcgcc ccgattacgg cgacatgctg
     2041 aaacgcggca ttggctatac gccagaaaac cgcaaagaag cggggatcat tccctggttg
     2101 ggcgttgacg aaaatacagt gctgaccaat cggcaaaaaa tcagcgccaa cggagtgctg
     2161 caatggtcca ccatccgccg cctgaccgaa gaggtgatgc agcggatgac ggtcaaggcc
     2221 gccagtagcg aaacgcccat cggcacgctt tctggtggca atcagcaaaa agtggtgatc
     2281 ggtcgttggg tctatgccgc cagccagatt ttgttgctcg acgagccaac gcgcggcgtc
     2341 gatatcgaag ccaaacagca gatttaccgt attgtccgtg agctggctgc cgaaggaaaa
     2401 agcgtggtgt ttatctccag tgaagtggag gagttgccgc tggtgtgcga ccgcatcctg
     2461 ttgttacagc acggtacgtt ctcgcaggag tttcactcgc cggtcaatgt ggatgagctg
     2521 atgtccgcca ttctgtctgt gcactgatga attttaccga ggaacctgaa gatgtctgct
     2581 tcgtcattat cattgccgct gggcaagagc gtctcgctca aacaatttgt cagtcgccat
     2641 attaatgaga tcggtttgct ggtggtaatc gccattcttt atctggtctt ctccctgaat
     2701 gcgcctggct ttatctcatt gaataaccag atgaacgtgc ttcgcgatgc tgccaccatc
     2761 gggatcgccg cctgggcaat gacgctgatt attatctccg gtgaaattga tgtcagcgtt
     2821 ggaccgatgg tggcttttgt ctcggtgtgc ctggcatttt tgctgcaatt tgaagttccg
     2881 ttggcgattg cttgtctgct ggtgttgctg ttaggcgcgc tgatgggaac gctcgccggg
     2941 gtgctgcgcg gcgtgtttaa cgtgcccagt ttcgttgcca cactggggct gtggagcgcc
     3001 ctgcgcggaa tggggctgtt tatgaccaac gcactgccag tgccgattga cgaaaacgag
     3061 gtgctggact ggctgggcgg acaatttctc ggtgtgccag tatccgcgct gatcatgatg
     3121 gtgttgtttg cgctgtttgt gttcattagc cgaaaaaccg ccttcgggcg ctcggttttt
     3181 gccgttggcg gtaatgccac ggcggcgcag ttgtgcggta ttaacgttcg tcgagtgcgc
     3241 attcttatct ttaccctttc gggattatta gcggcggtga ccggcatttt gctggcggca
     3301 cgcctcggtt ccggtaacgc aggtgccgca aacggtctgg agtttgacgt catcgccgcg
     3361 gtcgtggtcg gcggtacgga actttccggc ggtcgcggtt ccctgttcgg tacattgctt
     3421 ggtgtgctgg tgattacgct aatcggtaac ggtctggtgc tgctcggtat taactccttt
     3481 ttccagcagg tggtacgcgg cgtcatcatc gtggtggcgg tgctggcgaa tatcttgctg
     3541 acccagcgaa gcagtaaagc gaaacgctaa accttaacgc ccctggcgcg ggtgcggcag
     3601 ggatgaaaat taatcgtatt gttaatgagg gatttatgaa aacgatgctg gcagcttatt
     3661 taccaggaaa ttcaaccgtc gatctgcggg aagttgcggt gccgacgccg gggattaacc
     3721 aggtactgat caaaatgaaa tcctccggga tttgcggaag cgatgtccac tatatctatc
     3781 atcaacaccg tgccacggcg gcggcacccg ataaaccgtt ataccaggga tttatcaacg
     3841 gtcatgaacc atgcgggcag attgtggcga tggggcaagg ctgtcgccat tttaaagagg
     3901 gtgatcgcgt gctggtgtat cacatttctg gctgtggttt ttgcccgaac tgccgccgcg
     3961 gctttcctat ctcttgtact ggcaaaggaa aagcggctta cggctggcag cgtgacggcg
     4021 gtcatgccga atacttgctg gcggaagaaa aagatctgat cctcctgccg gatgcgctta
     4081 gctacgaaga tggtgcgttt atcagttgcg gcgttggtac agcgtatgaa ggaattttgc
     4141 gcggcgaagt ttccggcagt gataacgtgc tggtggtcgg tctggggcca gtcggcatga
     4201 tggcgatgat gctggcgaaa ggtcgcggtg caaaacggat catcggcgtt gatatgctgc
     4261 cggaacgtct ggcgatggca aaacagttag gggtgatgga tcacggctat ttagcaacca
     4321 ccgaaggtct gccgcagatt atcgccgaac tcacccacgg tggcgcggat gttgcgctcg
     4381 attgttctgg taatgccgca ggtcgcttgc tggcactgcg atccaccgct gattggggac
     4441 gggtggttta catcggagaa accggaaaag tggagttcga ggtcagcgcc gatctgatgc
     4501 accatcaacg gcggattatc ggctcctggg tgaccagtct gttccatatg gaaaaatgcg
     4561 cccatgatct gacggactgg aaactgtggc cgcgtaacgc cattacccat cgcttctcgc
     4621 tggaacaggc tggagatgcc tatgcgctga tggcgagcgg caaatgcggg aaagttgtga
     4681 ttaacttccc ggattaagga gttagcggat gaccatctat accttatctc acggaccgct
     4741 aaagctggat gtctctgacc agggcggcgt aatcgaaggc ttctggcgtg acacgacgcc
     4801 gcttttacgt cctggtaaaa aaagcggtgt ggcaaccgat gcttcctgtt ttccgctggt
     4861 gccgtttgca aaccgggtga gcggcaatcg ttttgtctgg caggggcgtg agtatcaact
     4921 gcaaccgaat gttgagtggg atgcgcacta tctacacggc gatggctggc tgggcgagtg
     4981 gcaatgtgtc tcgcacagcg atgatagctt gtgtctggtg tatgaacatc gcagcggtgt
     5041 ctatcactat cgggtaagtc aggcgtttca tttaacggcg gatacgctga cggtgacgct
     5101 ctctgtcacc aatcaagggg cagagacgct gccatttggt accggctggc atccttattt
     5161 tccgttgtca ccacaaacgc ggattcaggc gcaggcgagc ggttactggc tggagcggga
     5221 gcagtggctg gcgggtgagt tttgcgagca gctaccgcag gaactggatt ttaaccagct
     5281 cgcgccgttg ccgcgccagt gggtgaacaa tggttttgcc ggatggaatg gtcaggcccg
     5341 catcgagcag ccgcaggaag gttatgccat catcatggaa acgacgccac ctgcaccgtg
     5401 ttatttcatc tttgtttccg accctgcgtt tgataaagga tatgcgtttg atttcttctg
     5461 tctggaaccg atgagccatg cgccggatga tcatcatcgc ccggaagggg gtgatctcat
     5521 tgcgctagca ccaggggaat caacaacttc agagatgtcg ttgcgggtag agtggctgta
     5581 atttattaaa tgccggataa ggcgttcacg ccgcatccgg catctttcct aacgccgatc
     5641 gagagaaatt gcccccggcc cggtaattgc caatagcaag aacgcgccag cgatactgac
     5701 attcttccag aaattaatca tatttggccc aaccgcatcg ccggtcatat cccagtaatg
     5761 gtgaccaatc accgccgtac ccagcgtgta gaaaataaac agcaccgcca gcggacgggt
     5821 gaaaaagcca agcacgatta atatcgcggc gggcacttcc ataactaccg caataatcgc
     5881 tgccagcatc ggcattggcg cgcccaacga ggccatatat tggaccgtac cgtcaaagcc
     5941 catcattttg ggaaaaccca aaataatgaa aattaagacc acggcgatac gggcaattaa
     6001 taacaaaacg gggcgggcag ctccaaaatc aaaataacgt agtgtgttca taaccaatcc
     6061 ctttgcaaga taatgtgatt taaacgtaat acacactctt gcacctcgcc acgccccaag
     6121 cgttgagata ttcgctaatt atcctggtta cagtgattta agcgcgatgt tctcatctat
     6181 cagcagtttc gcatcaaacg ttttgccgct ctggatccat ttgcgaattg ggcgaatctc
     6241 acgcccctga ttcagcgtga ccgcaccgat aagcacgccg ttttgcagat taaaccaaat
     6301 cgccttctga gtttccgggt tgccacgaca aagccag
//
LOCUS       DADLFA010000200         6329 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000200 DADLFA010000000
VERSION     DADLFA010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6329
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.202"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..150
                     /locus_tag="JQH65_003572"
     CDS             <1..150
                     /locus_tag="JQH65_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY6140853.1"
                     /translation="AIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQ
                     PSRSL"
     gene            239..1699
                     /locus_tag="JQH65_003573"
     CDS             239..1699
                     /locus_tag="JQH65_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAY6140854.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYKVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(1735..1938)
                     /gene="ydfZ"
                     /locus_tag="JQH65_003574"
     CDS             complement(1735..1938)
                     /gene="ydfZ"
                     /locus_tag="JQH65_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HAY6140855.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(1935..2801)
                     /locus_tag="JQH65_003575"
     CDS             complement(1935..2801)
                     /locus_tag="JQH65_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005005969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY6140856.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIEHKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVARSA
                     RKTATGLAKSNSFTLIPSGMRGFSPRLTAHACIKKDVSLIKNKKYFTRQEYLYCARYV
                     GFDCKSERRKHL"
     gene            complement(2890..3636)
                     /gene="ydfG"
                     /locus_tag="JQH65_003576"
     CDS             complement(2890..3636)
                     /gene="ydfG"
                     /locus_tag="JQH65_003576"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HAY6140857.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3773..5818
                     /gene="dcp"
                     /locus_tag="JQH65_003577"
     CDS             3773..5818
                     /gene="dcp"
                     /locus_tag="JQH65_003577"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HAY6140858.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(5863..>6329)
                     /locus_tag="JQH65_003578"
     CDS             complement(5863..>6329)
                     /locus_tag="JQH65_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HAY6140859.1"
                     /translation="NLTSALSQRLIADQLRLTTAESCTGGKLASALCAAEDTPKFYGA
                     GFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAIERADADVSIAITGYGGLE
                     GGEDGTPAGTVWFAWHIKGQTYTAVMHFAGDCETVLALAVRFAHVQLLQLLL"
BASE COUNT         1583 a         1512 c         1628 g         1606 t
ORIGIN      
        1 gccattatga tcatggcata ttcagtgatt ggtttaatct ctgcgctgaa aatgccagag
       61 gtgaaagacc gtgatttaag tgcgctggaa gacgctgcgg aagatcaacc gcgtgttgta
      121 agagctgcgc aaccttccag aagtctgtaa accctcaatc ccttctctta ccggagaggg
      181 gatttttatt catataacaa aacatatagc ttgccatatt taaatttaag gaattgttat
      241 gggaaataat ttgttatcag caaaagcgac actccctgtt tatgatcgta ataacctggc
      301 cccaagaatt gttcatttag gctttggtgc atttcaccgt gcgcatcagg gcgtgtatgc
      361 cgatattctt gctacagaac atttcagtga ctggggatat tataaggtca acttaatcgg
      421 cggcgaacag caaattgccg atttacaaca gcaagataat ctttataccg ttgcggaaat
      481 gtcggccgat gcgtggacgg ctcgcgtcgt tggcgtcgtt aaaaaagcct tgcacgtaca
      541 gatggatggc ttagaaaccg tgttggctgc gatgtgtgaa ccgcaaatcg cgattgtctc
      601 tctgacaatc accgaaaaag ggtatttcca ctctccggcg accggacagt taatgctcga
      661 tcacccgatg gtcgctgccg acgtgcaaaa tccccaccag ccgaaaacag caacaggggt
      721 gattgttgag gcgctggccc gccgtaaagc ggcaggactt cccgcattta ccgtcatgtc
      781 atgtgacaac atgccagaaa acggtcatgt tatgcgtgac gttgtcactt catacgcgaa
      841 agctgttgat gtaaaactgg cacaatggat cgaagataac gtgactttcc catcaacaat
      901 ggtggaccgt attgtgcccg cagtgacaga ggatacgctg gcgaaaatcg aacaacttac
      961 cggtgtgcgc gatcctgcgg gcgttgcctg tgaacctttc cgccagtggg taatagaaga
     1021 taactttgtt gccggacgtc cagaatggga aaaagcggga gccgaactgg ttagtgatgt
     1081 gctgccttat gaagagatga agttgcgcat gctcaacggc agtcattcat tcctggcgta
     1141 tttgggttat cttgccggat atcagcacat taatgactgc atggaagatg aacattatcg
     1201 ttatgcggcg tatggcttga tgttgcagga acaagcgccg acgctgaaag tgcagggcgt
     1261 tgatttgcaa gattacgcta accgattaat tgaacgctat agcaatccgg cgttacgtca
     1321 tcgaacctgg cagattgcga tggatggtag ccagaaattg ccacagcgga tgttggattc
     1381 tgttcgctgg catctggcgc atgacagcaa gttcgatctg ctggcgctgg gcgtcgcggg
     1441 ttggatgcgt tatgtcggtg gtgttgatga acagggaaat ccgatagaaa tcagtgaccc
     1501 actgttacct gttattcaga aggctgtaca aagtagtgcc gaagggaaag cgcgcgtcca
     1561 gtcattgctg gcgattaagg caatctttgg tgatgatttg ccagacaata gcttgtttac
     1621 tgcaaaagtg acggaagcgt acttgtcttt attagcgcat ggcgcgaaag cgaccgtggc
     1681 gaaatattcc gtgaagtaaa aaaagcaatg ccgccatcgg cggcattcac acacttagtt
     1741 catgccgaga cgaatcaggt ccagaggtgc cagtttctct tcacaacctt caacaactac
     1801 ggtttttccg cggcggattt gctcagcggt cagaccttca gtatcaatcg acaggatctt
     1861 gccagtgtga ccggtgccgc taaccataac acggctgcca gtggtgattg cgttacggtt
     1921 acgatcgtag gtcgtcatag gtgttttctc ctttctgatt tacagtcgaa gcccacgtaa
     1981 cgggcgcaat ataaatactc ctgtcgagtg aaatattttt tgtttttgat caagctcaca
     2041 tcttttttga tgcatgcatg agccgtaagg cggggggaaa agcctctcat cccggatggg
     2101 atgagagtaa atgaattact cttcgctaaa ccagtcgctg ttttcctggc ggatctggcc
     2161 acggattcgc tgatttcctg caaatgttgc gtcattgcac gttctaccgc atcgccatca
     2221 cgtttttgca gggcagagaa aatatcaaga tgctggcgta acagcatttc tggtggagaa
     2281 acatggtcga aactcatata gcgcacgcga tcaacggtcg ctttcaggtt ctcaatggta
     2341 tcccacgcca gttgacagtc ggcaatctgc gtcaggagtt gatggaagtt gtcatcaagt
     2401 tcaaaaaaat catccagttg cttgtgctca atggcaatgc gttgctggtg aagattttgt
     2461 tccagttggt agcactggct ttcggtaatc atgctcgccg cccgacgcgc caccgcgcac
     2521 tcaatggcct gacggataaa actgccgttg cgcacctggg ccatggaaat tttgttgacg
     2581 tagctgccac gctgtggacg aatttgaatc aggccgtttt ccgccagttt aataaaggct
     2641 tcacgaaccg gctggcgtga cacattgaaa cgaacagaaa cttctttttc cgacaacggt
     2701 gtgcctggag caatcaggca atggacaatg tcgcgacgaa ggatacgata aatttgctga
     2761 ttgacaggct gtgtgggatt aagttgcgtt tcgacggtca ttcgttcttg cttgtgagtg
     2821 agttaactgc atgagtctac cacttttttg tgacaagcaa taacccggca gttacgccgg
     2881 gtataaaaat tactgacggt ggacattcag tccggcatag ctttgggtaa ccggcatcat
     2941 ttccagggta ttgatattga cgtgagcagg cagcgttgac acccaccaaa cggcttcgct
     3001 gacatcttct ggcgtcaatg caacggtatt ttgataggtt ttttccgctt taccgtcatc
     3061 gcctttaaag cggacattgg aaaactcggt gccacccacc agacccggtt cgatgtcggt
     3121 gacgcgcacc gccgtaccat gcaggtccgt acgcagattc aggctaaact gacgaacaaa
     3181 cgctttcgtc gcaccgtaaa cgttaccacc ggcataaggc cagctacctg ccgttgagcc
     3241 aatgttaata atatgaccat gattacgttc aaccataccc ggtaagacgg cgcgcgtcat
     3301 atataccagg cctttgttgt tggtatcaat catcgtttcc cagtcttcaa cgctggcttt
     3361 atgcgcaggc tccatgccca acgccaggcc ggcattattt accaggatat caatattgca
     3421 ccactcggca ggaagcgatg ccagcatctc ttcaatagcg gcgcggttgc gaacgtccag
     3481 ttgggcgatg tacagattat ctcccagttc gtcttttaac tcctgcaacc gttcctggcg
     3541 acggccagtg gcgataactt tatgcccttg ttgaataaaa cgacgagtaa tgcattcacc
     3601 aaaacctgcc gttgctccag ttactaaaac gatcatctca ctgttcctca acgcttttgt
     3661 gtgtcaataa catagcacgt gatgcttaag agcgggtaat actggtttgt aagattgcac
     3721 tccgttatca gtaagcctac gctatggaac acctcgcgtt caggagaaga aaatgacaac
     3781 aatgaatcct ttccttgtgc aaagcacact accgtatctg gctccccatt ttgatcaaat
     3841 tgccaatcat cactatcgcc cggcattcga tgagggaatg cagcaaaagc gggcagaaat
     3901 tgctgccatc gcgcttaacc cgcaaacgcc tgatttcaac aatactattc tggcactgga
     3961 acaaagcgga gaattactta cccgcgttac cagcgtcttt tttgcgatga ctgcggcgca
     4021 taccaatgat gaattacagc gtcttgatga acagttttcc gctgaactgg cggaactggc
     4081 taatgatatc tatctgaacg gtgaattatt cgcgcgggta gatgctgtct ggcagcgccg
     4141 tgaatccctg gggcttgata gtgaatccat ccgcctggtg gaggtgattc atcaacgttt
     4201 tgtccttgcc ggagccaaac ttgcgcaagc tgataaagca aaattaaaag tactgaatac
     4261 agaagctgcg accctgacca gccagtttaa ccagcgatta ctggcagcaa ataaatccgg
     4321 cggtctggtt gtgaacgata tcgcgcagct ggcaggaatg agtgagcaag agattgcgct
     4381 ggcggcagag gcggctcgcg agaaaggtct ggataacaaa tggctgattc cgctgctgaa
     4441 taccacccaa caaccggcgc ttgccgagat gcgcgatcgt gcgacgcgtg aaaaactgtt
     4501 tattgcgggc tggacgcgag cggaaaaaaa tgatgccaat gatacccgcg ctatcattca
     4561 acgtctggta gagattcgcg cacagcaggc gaaactgctt ggttttcctc attatgccgc
     4621 atggaaaatc gccgatcaga tggcaaaaac gccagaagca gcactcaact ttatgcggga
     4681 aattgttcca gcggcgcgtc aacgtgctag cgatgaatta gcctccatac aggcggttat
     4741 cgataagcag caaggcgggt ttagcgcgca gccgtgggac tgggcatttt atgccgaaca
     4801 ggtacggcgg gagaaatttg atcttgatga ggcgcagctc aagccatatt ttgaattaaa
     4861 cacggtgttg aatgaaggtg tattctggac cgcgaatcag ctcttcggta ttaagtttgt
     4921 cgaacgtttt gatattcctg tctaccatcc tgacgttcgt gtgtgggaaa tttttgatca
     4981 taatggcgtg ggactggcgt tattttacgg tgatttcttc gcccgtgatt caaaaagcgg
     5041 cggtgcatgg atgggcaatt ttgttgagca atcaacgctt aatgaaacgc atccggtaat
     5101 ttataacgtt tgcaattatc agaaacccgc tgccggtgag cctgcgttgt tactctggga
     5161 tgatgtcata accttattcc atgaatttgg tcatacgctg cacggccttt ttgcccgcca
     5221 gcgttatgcc acgctttccg gcaccaacac gccgcgtgat tttgtcgaat ttccgtcgca
     5281 aatcaacgaa cactgggcaa cgcatccgca ggtattcgct cgctacgccc ggcattatca
     5341 gagcggggca gcaatgcctg acgaactgca acagaaaatg cgtaatgcca gcctgttcaa
     5401 caaagggtat gagatgagcg aactgcttag cgccgcactt ctcgatatgc gctggcattg
     5461 cctggaagaa aacgaagcaa tgcaggatgt cgatgatttt gaattgcggg cgctggtggc
     5521 ggaaaatatg gatcttcctg ctataccgcc acgctatcgc agcagttatt tcgcccatat
     5581 ttttggtggc ggatatgctg caggttatta cgcttatctg tggacgcaaa tgttggccga
     5641 tgatgggtac cagtggtttg ttgagcaggg cggattaacg cgtgaaaatg ggcagcgttt
     5701 tcgcgaggcg atcctttcca gaggtaacag cgaagatctg gaacgcctgt atcgacaatg
     5761 gcgcggtaag gctcctcaga ttatgccgat gctgcaacat cgtggcttga atatataaac
     5821 accaccaatg gctggcctgg agatatcgcc aggccagcct ggttatagca gtaattgcag
     5881 cagctggacg tgggcaaacc tcaccgctaa agctaatacc gtttcgcagt cgccagcaaa
     5941 atgcataacc gcagtgtagg tctggccttt aatatgccac gcgaaccaga cggtacccgc
     6001 tggcgtacca tcttcaccgc cctccagtcc gccgtagccg gtaatggcaa tactgacatc
     6061 agcatccgca cgctctatgg cacctgttgc catttctgct gccactttct cactcaccgc
     6121 agaatatcgt tcaagagatt gctggcttac gctgaggatt ttcatctttg cctgatcggt
     6181 gaaagtaaca aagcctgcac cgtaaaattt gggtgtatct tcagctgcac acagggcgct
     6241 agccaactta ccgccggtgc atgattcggc ggtagttaag cgtaattgat ccgcgataag
     6301 tctttgactc aacgcggatg tcaggtttt
//
LOCUS       DADLFA010000201         6317 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000201 DADLFA010000000
VERSION     DADLFA010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6317
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.203"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            285..626
                     /gene="raiA"
                     /locus_tag="JQH65_003579"
     CDS             285..626
                     /gene="raiA"
                     /locus_tag="JQH65_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAY6140860.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     gene            730..777
                     /locus_tag="JQH65_003580"
     CDS             730..777
                     /locus_tag="JQH65_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6140861.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            877..2037
                     /gene="pheA"
                     /locus_tag="JQH65_003581"
     CDS             877..2037
                     /gene="pheA"
                     /locus_tag="JQH65_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HAY6140862.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2080..3201)
                     /gene="tyrA"
                     /locus_tag="JQH65_003582"
     CDS             complement(2080..3201)
                     /gene="tyrA"
                     /locus_tag="JQH65_003582"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HAY6140863.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLKQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3212..4282)
                     /gene="aroF"
                     /locus_tag="JQH65_003583"
     CDS             complement(3212..4282)
                     /gene="aroF"
                     /locus_tag="JQH65_003583"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HAY6140864.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDLETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4490..4855
                     /locus_tag="JQH65_003584"
     CDS             4490..4855
                     /locus_tag="JQH65_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HAY6140865.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFGDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVESASQ
                     LHEVWQKGADENASTIRLN"
     gene            5005..5523
                     /locus_tag="JQH65_003585"
     CDS             5005..5523
                     /locus_tag="JQH65_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HAY6140866.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAKQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5513..>6317
                     /gene="dgcN"
                     /locus_tag="JQH65_003586"
     CDS             5513..>6317
                     /gene="dgcN"
                     /locus_tag="JQH65_003586"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HAY6140867.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGIN"
BASE COUNT         1718 a         1612 c         1419 g         1568 t
ORIGIN      
        1 aaaaagagcc agtcagttgc ttaactgact ggcattacag cccttgagct gccgtttttt
       61 tattctgtta gttgtgaaac tgaagcgatt tagtcactat cgatctcatc aaatatggct
      121 cgctttgaga tattcctcaa gtaaaaaaac acctcttcct gcgatttctc acaaaaaaga
      181 ttcgttgaca aaaagtgaca aaattatgag attcccatca cacattttga catcaggaac
      241 ggtatgctga attcaccaag acgggaagac aagaggtaaa atttatgaca atgaacatta
      301 ccagcaaaca aatggaaatt actccggcca tccgccaaca tgtcgcagac cgtctcgcca
      361 aactggaaaa atggcaaaca catctgatta atccacatat cattctgtcc aaagagccac
      421 aagggtttgt tgctgacgcc acaatcaata cacctaacgg cgttctggtt gccagtggta
      481 aacatgaaga tatgtacact gcaattaacg aattgatcaa caagctggaa cggcagctca
      541 ataaactgca gcacaaaggc gaagcacgtc gtgccgcaac atcggtgaaa gacgccaact
      601 tcgtcgaaga agttgaagaa gagtagtcct ttatattgag tgtatcgcca acgcgccttc
      661 gggcgcgttt tttgttgaca gcgtgaaaac agtacgggta ctgtactaaa gtcacttaag
      721 gaaacaaaca tgaaacacac accgtttttc ttcgcattct tttttacctt cccctgaatg
      781 ggaggcgttt cgtcgtgtga aacagaatgc gaagacgaac aataaggcct cccaaaccgg
      841 ggggcctttt ttattgataa caaaaaaggc aacactatga catcggaaaa tccattactg
      901 gcgctgcgag agaaaatcag cgcgctggat gaaaaattat tagcattact ggcagagcgg
      961 cgcgaactgg ccgtcgaggt gggaaaagcc aaactgctct cgcatcgccc ggtacgagat
     1021 attgatcgtg aacgcgattt actggaaaga ttaattacgc tcggtaaagc gcaccatctg
     1081 gacgcccatt acattactcg cctgttccag ctcatcattg aagattccgt attaactcag
     1141 caggctttgc tccagcaaca tctcaataaa attaatccgc actcagcacg catcgctttt
     1201 ctcggcccca aaggttctta ttcccatctt gcggcgcgcc agtatgctgc ccgtcacttt
     1261 gagcaattca ttgaaagtgg ctgcgccaaa tttgccgata tttttaatca ggtggaaacc
     1321 ggccaggccg actatgccgt cgtaccgatt gaaaatacca gctccggtgc cataaacgac
     1381 gtttacgatc tgctgcaaca taccagcttg tcgattgttg gcgagatgac gttaactatc
     1441 gaccattgtt tgttggtctc cggcacgact gatttatcca ccattaatac ggtctacagc
     1501 catccgcagc cattccagca atgcagcaaa ttccttaatc gttatcctca ctggaagatt
     1561 gaatataccg aaagtacgtc tgcggcaatg gaaaaggttg cacaggcaaa atcaccgcat
     1621 gttgctgcgt tgggaagcga agctggcggc actttgtacg gtttgcaggt actggagcgt
     1681 attgaagcga atcagcaaca aaacttcacc cgatttgtgg tgttggcacg taaagccatt
     1741 aacgtgtctg accaggttcc ggcgaaaacg acgttgttaa tggcgaccgg gcaacaagcc
     1801 ggtgcgctgg ttgaagcgtt gctggtgctg cgcaaccaca atctgattat gacccgtctg
     1861 gaatcacgcc cgattcacgg taatccatgg gaagagatgt tttatctgga tattcaggcc
     1921 aatcttgaat cagcggaaat gcaaaaagca ttgaaagagt taggtgaaat tacccgttca
     1981 atgaaggtat tgggctgtta cccaagtgag aacgtagtgc ctgttgatcc aacctgatga
     2041 aaaggtgccg gatgatgtga atcatccggc actggattat tactggcggt tgtcattcgc
     2101 ctgacgcaat aacacgcggc tttcactctg aaaacgctgt gcgtaatcgc cgaaccagtg
     2161 ctccaccttg cggaaactgt caataaacgc ctgcttatcg ccctgcttca gcaactcaat
     2221 cgcctcgccg aaacgcttat agtaacgttt gattaacgcc agattacgct ctgacgacat
     2281 aataatgtcg gcataaagct gcggatcctg agcaaacagt cgcccgacca tcgccagctc
     2341 aaggcggtaa atcggcgaag agagcgccag aagttgctca agctgaacat tttcttctgc
     2401 cagatgcagc ccataagcaa aagtagcaaa gtggcgcaga gcctgaataa acgccatatt
     2461 ctgatcgtgc tcgacagcgc taatacgatg cagccgagcg ccccagacct gaatttgctc
     2521 cagaaaccat tggtatgctt ccggcttacg tccatcacac cagaccacaa cttgctttgc
     2581 caggctaccg ctgtccgggc cgaacatcgg gtgtaacccc agtaccgggc catcgtgcgc
     2641 cgccagcatg gcctgtaatg gtccattttt cactgatgcc agatcaacca gaatacaatc
     2701 tttcggtaaa ggcggtaatt tgccgataac ttgctcagta acgtggattg gcacactaac
     2761 aatcaccatt ccggcatcgg caacaatatc agccgctcga tcccagtcat gttgctccag
     2821 aatccgcacc tgataacccg agagtgtcag catcttctcg aacaggcgtc ccatctgacc
     2881 gccaccgccg acgataacca ccggacgcag tgacggacaa agtgttttaa atcctttgtc
     2941 gttttcactg gagtaagatt cacgcatcac ccgacgcaaa acatcctcaa tcagatctgg
     3001 cggtaccccc agagcttccg cctctgcacg acgcgaggcc aacatagatg cctcgcgctc
     3061 cggaacataa ataggcagtc caaagcggct tttcacctcg cccacttcag caaccagttc
     3121 cagacgcttc gctaataaat tcagcagcgc tttatcgact tcatcaattt gatcgcgtaa
     3181 tgcggtcaat tcagcaacca taataaacct cttaagccac gcgagccgtc agctgcccgt
     3241 tcagatcctg atgaatttca cgcagcaagg catcggtcat ttcccagcta atgcaggcat
     3301 cggttacgga tacaccgtat ttcatttcac tgcgcggttg ctcagaagac tgattgccct
     3361 cgtggatatt actttcgatc atcagaccaa taattgagcg attgccatct ttgatttgag
     3421 caaccacgga ttctgccacc gcaggctgac ggcgataatc tttattggaa ttaccgtggc
     3481 tgcaatctac catcagagac gggcgcagtc ccgcctgttc catctctttt tcacattgcg
     3541 caacatccgc agggctatag ttcggcgctt taccaccgcg caggatgaca tgcccgtccg
     3601 gattcccctg agtttgtagc aacgcaacct gccctgcctg gttaatgcca acaaaacggt
     3661 gcggctgggc ggcggcgcgc atagcgttaa ttgctgttgc cagactgccg tcggtgccgt
     3721 ttttaaaacc aaccggcatg gaaagcccgg aggccatttc acggtgagtt tgcgattccg
     3781 ttgtacgagc accaattgct gaccagctaa acagatcgcc caggtattgc gggctattcg
     3841 gatctaacgc ttccgtcgcc agtggcagtc ccatattcac cagctcaagc agcaatttac
     3901 gcgcgatctg cagcccggct tctacatcaa aagagccatc catatgggga tcgttaatta
     3961 accctttcca gccgacagtg gtacggggtt tttcaaaata gacgcgcatt accagataga
     4021 ggctatcgct gacctctgcg gcaagggctt taaatcgacg agcatattcc agagcagttt
     4081 ccagatcatg aatggaacaa ggaccacaaa ctaccagcag acgaggatcg cgcccggcga
     4141 taatatctga aatggtttta cgcgagtcag caatctgggc ttcttgttgc aggctcaatg
     4201 gaaaagcggc cttcagttgt tccggagtca ttaaaacctg ttcgtcggta atatgtacgt
     4261 tattcagcgc gtctttttgc atgatggcga tcctgtttat gctcgtttgc gatagttgat
     4321 cctcagcgag gatgacgtaa cgataacaca taaagtaaag ttttcaatcc atatttcgta
     4381 cgcttttctt tacacgaaca atttactcgc gctttgatgc ttatttcgta caaataaatt
     4441 tacaccccct ttaaaaacaa tccgctatgc tttgaaaaag gagaaagaaa tgatgaaaaa
     4501 gtttatcgcc cccttattgg ctttactggt tagcggatgt cagattgatc cttatactca
     4561 cgcgccaacc ttgaccagca ccgactggta tgatgtcggt atggaagatg cgatatcggg
     4621 cagcgccata aaagatgacg atgcatttgg cgattcacag gcggatcgcg gtctatacct
     4681 taaaggatat gccgaaggac aaaagaaaac ttgccagacc gattttactt atgcccgggg
     4741 actttccggt aaaagctttc ctgccagctg taacaatgtt gaaagtgcca gccaactgca
     4801 tgaagtatgg caaaaagggg ctgatgaaaa tgccagcaca atacgattaa attaattaaa
     4861 accccacaaa taaattaaac ataagatttt actcattgtg ttttatttct cacattgatg
     4921 acgatcgcat gaaaaaatga tattttaatg agcattctga aaaaatatcg atgcatttcg
     4981 agcgaagaag gtggggatcc ctgaatgcgt ttttctcacc gactgttctt acttctgatt
     5041 ctcctgctga cgggagcacc aatactcgct caggagcctt ctgacgttgc gaaaaacgtg
     5101 cgtatgatgg tttccggtat tgtcagttac acccgctggc ctgcgctatc agggccacca
     5161 aagttatgta tcttttcatc ttcacgtttt agtaccgccc tacaagagaa tgccgcgaca
     5221 tctttgcctt atctgcctgt catcattcac actcaacaag aggcgatgat ttcaggctgc
     5281 aatggttttt attttggcaa cgagtcaccg acattccaga tggaattaac cgaacaatat
     5341 ccgtcaaagg cgttattatt aattgccaaa caaaataccg agtgcattat tggtagcgcc
     5401 ttttgtctga tcatccacaa taatgacgtc agatttgccg taaacctgga tgccttatcg
     5461 cgtagcggtg taaaggtcaa cccggatgtc ttaatgctcg cacggaagaa aaatgatgga
     5521 taacgataat tctcttaata agcgccccac gtttaaaaga gcattacgca acatcagtat
     5581 gaccagcata tttatcacta tgatgctgat ctggttgctg ctttccgtga cctcggtgct
     5641 gaccctgaaa cagtacgcgc aaaaaaacct ggcactgaca gcagcaacaa tgacttacag
     5701 tctggaagca gctgtcgttt ttgccgatgg ccctgcagca actgaaacac tggcagcgct
     5761 gggccagcaa gggcaatttt caactgcaga agtacgtgat aagcagcaaa atattctggc
     5821 gtcctggcat tacacccgta aggatccagg cgatactttc agcaatttca taagccactg
     5881 gctcttcccc gcccccatca ttcagccgat tcgtcacaat ggtgaaacca ttggcgaagt
     5941 acgcttaacc gctcgcgaca gttcaatcag ccattttatc tggttttcgc tcgccgtact
     6001 gaccggttgt attctgctgg catcaggcat cgcaattacc ctcacccgcc atttgcacaa
     6061 tggcctagtg gaagcgctga aaaatatcac cgatgtcgta catgatgtgc gttccaaccg
     6121 caatttttcc cgacgagttt ctgaagaacg tatcgctgag tttcaccgct tcgctctcga
     6181 cttcaacagt ctgctggatg aaatggaaga gtggcagctt cgtttacagg ccaaaaatgc
     6241 gcagcttctg cgtaccgcgc tacatgaccc attaaccggg ctggctaacc gcgcagcgtt
     6301 tcgtagcggc atcaata
//
LOCUS       DADLFA010000202         6294 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000202 DADLFA010000000
VERSION     DADLFA010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6294
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.204"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(85..621)
                     /gene="ssb1"
                     /locus_tag="JQH65_003587"
     CDS             complement(85..621)
                     /gene="ssb1"
                     /locus_tag="JQH65_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HAY6140868.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            875..3697
                     /gene="uvrA"
                     /locus_tag="JQH65_003588"
     CDS             875..3697
                     /gene="uvrA"
                     /locus_tag="JQH65_003588"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAY6140869.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3732..4088)
                     /locus_tag="JQH65_003589"
     CDS             complement(3732..4088)
                     /locus_tag="JQH65_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HAY6140870.1"
                     /translation="MTISELLQYCMAKLGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4092..4508)
                     /locus_tag="JQH65_003590"
     CDS             complement(4092..4508)
                     /locus_tag="JQH65_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HAY6140871.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMQGTSLVL
                     PLHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4619..5332)
                     /locus_tag="JQH65_003591"
     CDS             complement(4619..5332)
                     /locus_tag="JQH65_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B acid phosphatase"
                     /protein_id="HAY6140872.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5695..6237
                     /locus_tag="JQH65_003592"
     CDS             5695..6237
                     /locus_tag="JQH65_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005059113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6140873.1"
                     /translation="MVINFITRDGDDDMNISYMNSNKTTSLPVELDALNNKDISYAKD
                     FSYVKDFFLYIETQLKIAKDFCRLGEEVSSSIASKVFHAFIDLVNKIRGKKDFMYICT
                     LCCFAEEVKGDYSHYRTFLFDIGNQYKVKLTQSGKKEFSLTLEFNDTIIESQKVTGNK
                     AKHILEDIEIFYRNKPDTYY"
BASE COUNT         1594 a         1554 c         1569 g         1577 t
ORIGIN      
        1 cataacggag gcttcttttt tatggctggc agatgcttta atcatccacc ttaaaacaat
       61 ataacctatt gttttaatga caaatcagaa cggaatgtca tcatcaaagt ccatcggcgg
      121 ctcgttagac ggcgctgccg gagcggactg ctgcgggcga gactgcgcgc cgccgctgaa
      181 ctgattgcca ccctgcggct gctgaggctg accccaaccg ccctgcggct gaccaccacc
      241 gatattacca cctgccggag cgccaccacc ctgacgacca cccagcatct gcatggtgcc
      301 gccaacgttc accacgactt ctgtggtgta gcgatcctga ccggattgat cggtccattt
      361 acgggtacgc agctgacctt cgatataaac ctgagaacct ttacgcagat attcgctggc
      421 cacttctgcc agtttgccga acagcacaac gcggtgccat tcagtctgtt ctttcatctc
      481 gccggtcgct ttatcacgcc aggattcgga agtagccagc gtaatgttgg caactgcgcc
      541 accatttggc atgtagcgta cttccgggtc ctgacccaga ttaccaacga gaataacctt
      601 gtttacgcct ctgctggcca tgttcgtgtc tcctgaaaaa aatcgttctg aataagtgta
      661 aacgcgcgat tgtaccatta ccaatagcgc ttttactatg ttgtgacctc ggttccggga
      721 aacaaacctg gccagacatt gttacacaac actccggata atgcattcca atactgtata
      781 ttcattcagg tcaatttgtg tcataattaa ccgtttgtga tcgccggtag caccatgcca
      841 ccgggcaaaa aagcgtttaa tccgggaaag gtgaatggat aagatcgaag ttcggggcgc
      901 ccgcacccat aatctcaaaa acatcaacct cgttatcccc cgcgacaagc tcattgtcgt
      961 gaccgggctt tcgggttctg gcaaatcctc gctcgctttc gacaccttat atgccgaagg
     1021 gcagcgccgt tacgttgaat ccctttccgc ctacgcgcgg cagtttctgt cactgatgga
     1081 aaagccggac gtcgatcata ttgaggggct ttctcctgcc atctcaattg agcagaaatc
     1141 gacgtctcat aacccgcgtt ctacggtggg gacaatcacc gaaatccacg actatttgcg
     1201 tttgttgttc gcccgcgtcg gcgaaccgcg ctgtccggac cacgacgtcc cgctggcggc
     1261 gcaaaccgtc agccagatgg tggataacgt gctgtcgcag ccggaaggca agcgtctgat
     1321 gctactcgcg ccaatcatta aagaacgcaa aggcgaacac accaaaacgc tggagaacct
     1381 ggcaagccag ggttacatcc gtgctcgtat tgatggcgaa gtctgcgatc tttccgatcc
     1441 gccgaaactg gaactgcaaa agaaacacac tattgaagtg gtggttgatc gcttcaaggt
     1501 gcgtgacgat cttacccaac gtcttgcgga gtcgtttgaa accgcgctgg agctttccgg
     1561 tggtacagcg gtagtggcgg atatggacga cccgaaagcg gaagagctgc tgttctccgc
     1621 caacttcgcc tgcccaattt gcggctacag tatgcgtgaa ctggagccac gactgttttc
     1681 gtttaacaac ccggcaggtg cctgcccaac ctgtgacggc cttggcgtac agcaatattt
     1741 cgatcctgac cgcgtgatcc aaaaccccga gctgtcactg gctggcggtg cgatccgtgg
     1801 ctgggatcgc cgcaacttct attacttcca gatgctgaaa tcgctggcag atcactataa
     1861 gttcgacgtc gaagcgccgt ggggcagcct gagcgcgaac gtgcataaag tggtgttgta
     1921 cggttctggc aaagaaaaca ttgaattcaa atacatgaac gatcgtggcg atacctccat
     1981 ccgtcgtcat ccgttcgaag gcgtgctgca caatatggag cgccgttata aagagacaga
     2041 atccagtgcg gtacgtgaag aattagccaa gtttatcagc aatcgcccat gcgccagctg
     2101 cgaagggacg cgtctgcgtc gggaagcgcg ccacgtttat gtcgagaata cgccgctgcc
     2161 tgctatctcc gacatgagca tcggtcatgc gatggaattc ttcaacaatc tcaaactagc
     2221 tggtcagcgg gcgaagattg cagaaaaaat ccttaaagag atcggcgatc gtctgaaatt
     2281 cctcgttaac gtcggcctga attacctgac actttcccgc tcggcagaaa cactttccgg
     2341 cggtgaagcc cagcgtatcc gtctggcgag ccagattggt gcgggcctgg ttggcgttat
     2401 gtatgtactg gacgagccgt ctatcggcct gcaccagcgc gataacgagc gcctgttggg
     2461 tacgcttatc catctgcgcg atctcggtaa taccgtgatt gtggtggagc atgacgaaga
     2521 cgcaattcgc gccgctgacc atgtgatcga tatcggtcct ggtgcgggtg ttcacggcgg
     2581 tgaagtggtc gcagaaggtc cgctggaagc gatcatggcg gtgcctgaat cgttgaccgg
     2641 gcagtacatg agcggtaaac gcaagattga agtgccgaag aaacgcgttc cggcgaatcc
     2701 agaaaaagtg ctgaagctga caggtgcacg cggcaacaac ctgaaggacg tgacgctcac
     2761 gctgccagtc ggtctgttta cctgcatcac aggggtttca ggttccggta aatcgacgct
     2821 gattaacgac acactgttcc cgattgccca acgccagttg aatggtgcga ccatcgccga
     2881 accggcaccg tatcgcgata ttcaggggct ggagcatttc gataaagtga tcgatatcga
     2941 ccaaagccca attggtcgta ctccacgttc taacccggcg acctataccg gcgtgtttac
     3001 acctgtgcgc gaactgtttg cgggcgtacc ggaatcccgt gcgcgtggtt atacgccagg
     3061 acgtttcagc tttaacgtcc gtggcgggcg ctgcgaagcc tgtcagggcg acggtgtgat
     3121 caaagtggag atgcacttcc tgccggatat ctacgtgccg tgcgatcagt gcaaaggtaa
     3181 acgctataac cgtgaaacgc tggaaattaa gtacaaaggc aaaaccatcc acgaagtgct
     3241 ggatatgacc atcgaagagg cgcgtgagtt ctttgatgcg gtgccagctc tggcgcgtaa
     3301 gctgcaaacg ttgatggacg ttggtctgac gtacattcgc ctggggcagt ccgcaaccac
     3361 actttctggt ggtgaagccc agcgcgtgaa gctggcgcgt gagctgtcaa aacgcggcac
     3421 cgggcagacg ctgtatattc tcgacgagcc gaccaccggt ctgcacttcg ccgatattca
     3481 gcaactcctc gacgtgctgc ataaactgcg cgatcagggc aataccattg tggtgattga
     3541 gcacaatctc gacgtgatca aaaccgctga ctggattgtc gacctgggac cagaaggcgg
     3601 cagtggcggc ggcgaaatcc tcgtctccgg tacgccagaa accgtcgcgg agtgcgaagc
     3661 ttcgcatacg gcacgcttcc tcaagccgat gctgtaatcg ttaaggccgc tttctgagcg
     3721 gccttttcct ttcagagttg taccagcaat ttacgctttt cttccggtag taaattcact
     3781 gcctgctgat aagacgcatc caccagataa tagatttgcg aatccggcag cgaaccgtcg
     3841 agatacacgg tgctccagtg cgctttattc agatggcggc taggacgcac atcgctgtgc
     3901 tgctgacgta gcagctccgc cagctccggg ctggttttca gcgaaacagc tgggcgattt
     3961 tctacttctt tcaccatcgc aaacagtaca tcttccactt tgatctgcgt cgctttccag
     4021 tcgttatgca cgctctgttc tgcgcctagt tttgccatgc aatattgtag caactccgaa
     4081 atggtcattt tttactcccc ttgtagtgtc gcgatgatgc gacgcgatcc gccgtggatg
     4141 cgatgttccc ccagccagat gccttgccag gtgccggtct gaatacgccc tttatgtaac
     4201 ggcaatacaa gcgatgttcc ctgcattgag gatttgatat gagaaggcat atcgtctgct
     4261 ccctcatagt catgctcata atttccgttg tcgggaacgg tgcggaggaa aaaacgctcc
     4321 atgtcgtggc gtacggtggg atcgcagttc tcattaagtg tcagagaggc ggaggtatgt
     4381 tgcagcaaca gatgcagtaa gccgatgtta acgcgcggca tatcagccag ctgattcaga
     4441 atttcatccg ttaccagatg aaacccacga gatttggcgc taagcgtgag cgtcttttga
     4501 taccacatgt gctgctcctt gataaaactc tcttaatcag tttgcagcaa gacagcgaaa
     4561 ggataaaggt gtgattaaaa aaacagcatt cgggagagca tcacgctctc ccgctctgtc
     4621 agtattctga attgacgatc acctcttcac caaacgcacc cgcttgtggc aagggtttgt
     4681 aggtagagtt ggaggcgcgc agaatgcgga taccacgagc gccgacatcg cgtgcggcgg
     4741 taatatcatt atcagaatcg ccataaaaaa ttcggatatt tttatcctgc agccattgcg
     4801 attttgtatt ttgccctggt ttatcgcccg caaagatcac cggattcatg ttggtggcag
     4861 gaatatgaaa attatccgcc agcgtttttg aaaccgtttc tgttttcgtc ggactacgac
     4921 cagtcacaaa gaagatcgcg tcaccgcggc gtacatgcat atcaatcagc tggcgagcga
     4981 cctcttttgg aatgctgaat tcatcccagc cattgttcat tttttcccag aacacaggat
     5041 ttttcagata atcttcgctt tctggcgaga aggttttttt gccgcgccag aagcccgggc
     5101 tggaaaaaag taccgtgtca tcgatatcaa accccaccgc cattggcgga cgccctgcga
     5161 ggctattttc aatttgtgcg accgaaaccc aatgaattgg tgcctgttca gcaagcctgg
     5221 caacgttagt accagggtta agcggtgaag gagatgaggc cagggcaaca gcggaactgt
     5281 ttagagcgaa caataagcaa acggcactga ttgcctgtgt gatcttgcgc atatttttcc
     5341 ctaaatagtc agtatgttga aactttttat tgtgagattt gttgcaaaaa ccatctgacc
     5401 ataacgccag ctgcagggga aaggaaggat tatctgcggt ttttgtgatg aaaagcagga
     5461 tgattatatt taacccagta aatacgttcc tgtctttttt tgtataaaaa atgtttatta
     5521 ctcgatgtaa ggatacatta attttacgtt tattatgatg aatcctctgc aaatgagcga
     5581 aatttgatag aacatttatc tgctgtacga tttaatgaac ttaatgatac agcattacaa
     5641 aaacaatcga agtttataaa gatgattcct gattgaccag aaccctatcc tacaatggtg
     5701 attaatttta ttactcgcga tggagatgat gatatgaata tatcatatat gaatagcaac
     5761 aaaacgacat ctttacctgt tgagctagat gcactaaata ataaagatat ctcttatgcc
     5821 aaagattttt cttatgtcaa agatttcttt ttatacatag aaacacagtt aaaaattgca
     5881 aaagattttt gtagacttgg agaagaagta tcaagttcta ttgcaagtaa agtttttcac
     5941 gcatttattg atttagttaa caaaatcagg ggtaagaaag actttatgta tatttgcacg
     6001 ctttgctgtt ttgccgagga ggttaaaggt gattattctc attacaggac ctttttattt
     6061 gatattggta atcaatataa ggttaaactt acacaaagcg gaaaaaaaga gttctcttta
     6121 acattagaat ttaacgatac tataattgaa tctcagaaag tcacaggcaa taaggcaaag
     6181 catattcttg aggatataga aatattctat cgtaacaaac ccgatactta ttattaaatt
     6241 taataatggt tagatgtgca gaaattatta ctccacacga aaaaataggg aaag
//
LOCUS       DADLFA010000203         6186 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000203 DADLFA010000000
VERSION     DADLFA010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6186
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.205"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..147
                     /locus_tag="JQH65_003593"
     CDS             <1..147
                     /locus_tag="JQH65_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HAY6140874.1"
                     /translation="LPGQANRFYEGAISEHLQIGIRAIDLLRAEGDRLHSRKLRTFNE
                     PPFR"
     gene            490..1206
                     /locus_tag="JQH65_003594"
     CDS             490..1206
                     /locus_tag="JQH65_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY6140875.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(1662..1737)
                     /locus_tag="JQH65_003595"
     tRNA            complement(1662..1737)
                     /locus_tag="JQH65_003595"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1702..1704),aa:Asn,seq:gtt)
     gene            complement(1838..3481)
                     /gene="yeeO"
                     /locus_tag="JQH65_003596"
     CDS             complement(1838..3481)
                     /gene="yeeO"
                     /locus_tag="JQH65_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HAY6140876.1"
                     /translation="MLRHILTAKNLLSNPIFKFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSLFEMWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKVLALTYLEL
                     TVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSWPGLGFV
                     GAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPASVE
                     SVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGVG
                     QIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIWLNALF
                     MPIWSASWGLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGVWMGMF
                     ADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
     gene            3486..3561
                     /locus_tag="JQH65_003597"
     tRNA            3486..3561
                     /locus_tag="JQH65_003597"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3519..3521,aa:Asn,seq:gtt)
     gene            3723..4985
                     /locus_tag="JQH65_003598"
     CDS             3723..4985
                     /locus_tag="JQH65_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HAY6140877.1"
                     /translation="MSLNDSKIRNLKPSSKPVKLSDSHGLYLLVNQGGSRIWYLKYRF
                     SGKESRVSLGAYPLVSLAEARQRRDDIRKLLTQNINPAHQRMSDKAAASPEKYFKAVA
                     LAWHKTNKKWSPDYAARILASMENHIFPAIGHLPVTTLKTQNFTALLRVIEDKGFLEV
                     ASRTRQQLCNIMRYAVQQGFTENNLAQHLEGVTAPPVKNHYPALPLERLPELFERIGD
                     YQQGRQLTRLAVVLTLHLFIRSSELRFARWGEIDFRNKIWTIPATREAIEKVRFSGRG
                     AKMRTPYIVPLSRQAIAILKQIKEISGHLELVFPGDHNPYKPMSENTVNRALRLMGYD
                     TKTDVCGHGFRTMACSALVETELWSRDTVERQMSHQERNSVRAAYIHRAEHLDARTAM
                     MQWWSDYLDVSREGYVAPYIYARRHKAA"
     gene            complement(5704..>6186)
                     /locus_tag="JQH65_003599"
     CDS             complement(5704..>6186)
                     /locus_tag="JQH65_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY6140878.1"
                     /translation="MAAALELSRLMGLRSQEVVQSAQSLRTWKQSLERGEPRLTVVFG
                     TKGGRPRETVILDAVAIRKALDNALVVAEDRHGRLIDKPDLKSAMKYWHSQASRLGLT
                     GTYSPHSLRYAWAQDAIRHYLAQGFSEKEALAMTAMDLGHGDGRGRYVAQVYGRKDTD
                     "
BASE COUNT         1781 a         1505 c         1337 g         1563 t
ORIGIN      
        1 cttcccggcc aggctaaccg tttttatgaa ggcgctattt ccgaacatct gcaaattggc
       61 attcgggcga tcgatttgct gcgcgcagaa ggcgaccgac tgcattcgcg taaattacga
      121 acctttaatg agccgccgtt ccgataataa gttctgaacc ttcttatcag aaatagatgt
      181 gaaggagtaa gtaagacacc tggcaaatag cctgcaataa tcgtgggctg tttgcttcct
      241 tgggcggata cgtgttttat tatcgtctta atgatttcca catattaaaa gcaagtatgc
      301 tttcaaaaca caattataaa aaatcccgcc aacaatataa gtttttataa aattaaatat
      361 aagattatgg ctttagaata tttttatttc taatagacga gatttttcct gttatgatat
      421 aatatgccga attaacacat gttaacgatt taccagtaat gtaaataaat tttcgaggag
      481 atcattccag tgggacgtaa atgggccaat attgttgcta aaaaaacggc taaagacggt
      541 gcaacgtcta aaatttatgc aaaattcggt gtagaaatct atgctgctgc taaacaaggt
      601 gaacccgatc cagaattaaa cacatcttta aaattcgtta ttgaacgtgc aaagcaggca
      661 caagttccaa agcacgttat tgataaagca attgataaag ccaaaggcgg cggagatgaa
      721 acgttcgtgc agggacgtta tgaaggcttt ggtcctaatg gctcaatgat tatcgccgag
      781 acattgactt caaatgttaa ccgtacgatt gctaacgttc gcacaatttt caataaaaaa
      841 ggcggcaata tcggagcggc aggttctgtc agctatatgt ttgacaatac tggtgtgatt
      901 gtatttaaag gatcagaccc tgaccatatt tttgaaattt tacttgaagc tgaagttgat
      961 gttcgtgatg tgactgaaga agaaggtaac attgttattt ataccgaacc tactgacctt
     1021 cataaaggaa tcgcggctct aaaagcagct ggaatcactg agttctcaac aacagaatta
     1081 gaaatgattg ctcaatctga agttgagctt tccccagaag atttagaaat ctttgaaggg
     1141 cttgttgatg cccttgaaga tgacgacgat gtacaaaaag tttatcataa cgtcgcaaat
     1201 ctctaattat atattaaaga aatctgtctt tatggcagat ttctttaatc tcatataatc
     1261 cttataaaaa atataatgtt caactcgtca tattgcttat aaaaaatcct cgttcccaga
     1321 gaaataatat ttattataat tccagttctt ctttttctga ttacagaagg caaagtgaca
     1381 attacgcata gtttcccgat aaagacgcga cagctacatc ccgcataagg catttttctc
     1441 tttatccttg tacggtactt catggaacag agtttttgac cttgcgaatc gtgatgtctg
     1501 ttggggaggg gcaatttgct cactgaagcg tgagactcga ttaagcgcac gaaacacaga
     1561 aatcaaaaaa cccgggcact tttttacaag gtaaccgggt aaaaataatt tttatttttt
     1621 aactgttttg agactcatag agatgtctca aaactaaaat ttggctcctc tgactggact
     1681 tgaaccagtg acatacggat taacagtccg ccgttctacc gactgaacta cagaggaatc
     1741 gtgagaacga ggcgaatatt agcgatgccc acccacaatg tcaaagcctg ttatttaaat
     1801 ttgaaatcgt ttgctgaaat aatctgcatt ttgtcgttta ttccgacaca actggctttt
     1861 tttcacactt ttgcggttcg gatcgagggt atttccacag ccaacgtcca gtaaccattc
     1921 gccagtaaaa cagcacggcc cgcacagccc agtcggcaaa cattcccatc cagacaccaa
     1981 ccacacccca gccaagcatg atgcccagca cataaccgac gacaacccgg caaccccaca
     2041 tgctcaacat agaaacccac atggcgtaac gggcatcgcg agcaccttta aatccagcgg
     2101 gtagccccca tgaggcggac caaataggca taaataaagc atttagccaa atcagaatca
     2161 caacgacatg tttaacctgt ggatcctggg tgtaaaacga tgccataacc ccggcaaagg
     2221 gagccgttag ccaggcgatg gccgttaatc caagagtgga aagccagaac acatgccgca
     2281 actgaatctc tgcttgcgct atctgcccta cccccaacct tcggcctgta atgatcgtag
     2341 aagcagagcc gagcgcactt ccgggtaagt tgataagagc cgcaattgaa aacgcgataa
     2401 aatttccggc aataacactg gtccccatcc cggcaacgaa catttgggtt aataaccgac
     2461 cactggtaaa taacactgat tcgacactcg cgggaatacc aatccccatg acttcccaga
     2521 taatgctaaa attcagcggt ttaaaatagc tctttaacga aatccttagc gcaggattaa
     2581 aaccaatcgc cagcacccac aaaattgcaa ctgcgccaat ataacgagaa atggttaaac
     2641 ccagccctgc cccgacaaat cccagtcccg gccaggagaa aaggccgtaa atcaatatgc
     2701 cgctaataat aatattaaga atattcaggc taccgttaat caatagcggt attttcgtat
     2761 tccctgcacc acgaagtgcc ccgctaccaa taagagtgat ggcagctgct ggataactga
     2821 gtaccgtcag ctccagataa gtcaacgcca gtactttaac ttctgtcgtg gcatcacccg
     2881 cgacgaaatc aataatttgt tcgccaaaat gatgaataag cgttgccaac agtacggcaa
     2941 acaacgtcat gatcaccaat gactgccgcg tcgccaccct cgctcgtcgt cgatcccgct
     3001 taccgagact aaatgccaca acgacagtag taccaagatc gatagcagca aaaaaagcca
     3061 taatgaccat attgaagctg tccgccaatc ccacgccggc catcgcatct tttcccagcc
     3121 agctgaccag aaaagtgctc agaaccccca tcaacaggac acaggcattc tccatgaaga
     3181 taggaacagc aagcggggtt atctcgcgcc agaacaacac tttgtagctc ttgcgtttag
     3241 cgtgccagcg agtgccgtga acaacctggc gtaaagcaga ggagatattc aaagccgacc
     3301 ttaattgcag aaagtgaaac cacatttcaa ataatgaggg agaatcagca aagctgcaaa
     3361 gattttcgcc aacaaattgt ctgcaaatgc aacaaactgt tgatagaaac ggcaaacagt
     3421 tggggaattt aaaaatcggg tttgacaaaa gatttttcgc cgttaagatg tgcctcaaca
     3481 gcgattcctc tgtagttcag tcggtagaac ggcggactgt taatccgtat gtcactggtt
     3541 cgagtccagt cagaggagcc aaattccgat tcttacgcta tcttattagt ccttatttga
     3601 tattgattca ataagttagc gtgaaaatct ttccagatgt gtattcagtt ttccctccgc
     3661 atccggttaa tttggtggtc agatttgggg gcaggttggt tcgattctgg agtgaccccc
     3721 aaatgtcttt aaacgattca aaaatccgta acttaaaacc ctcttcaaaa cccgtaaaac
     3781 tgtctgattc gcacggtctg tatctgctgg tcaatcaggg cggttcgcgt atctggtatc
     3841 tcaagtatcg cttcagtggt aaagagtccc gcgtcagtct gggcgcatat cctctggttt
     3901 cacttgctga agcaagacaa cgacgtgacg atatccgtaa gctactgacg cagaatatta
     3961 accccgccca ccagcgcatg tctgataaag ccgcagcgtc accggaaaag tattttaaag
     4021 ccgtggcgct tgcatggcac aaaaccaaca aaaaatggtc gcctgattat gccgcgcgca
     4081 tcctcgccag catggaaaat catatctttc cggcgattgg tcatctgcct gtcactacgc
     4141 tgaaaacgca gaatttcact gcgttactgc gggttattga ggataaaggt tttctggaag
     4201 tcgcgtcccg aacccggcag caactctgca acatcatgcg ttacgccgta cagcagggat
     4261 tcaccgaaaa caacctggcg cagcatctgg aaggcgtcac cgcgccgccg gttaagaatc
     4321 actatcccgc cctgccgctg gagcggttgc ccgaattgtt tgagcgcatt ggcgactatc
     4381 agcagggacg gcagcttacg aggctggcgg tggtgttgac tctccacctg tttatccgtt
     4441 ccagcgagct gcgctttgcc cgctggggtg agatagattt cagaaacaaa atctggacca
     4501 tccccgccac aagggaagcg attgagaaag tccgtttctc tggccgtggc gcaaaaatgc
     4561 gtactccata tatagtgccg ctttctcgtc aggctatcgc cattctgaag cagataaagg
     4621 aaatctccgg ccatctggaa ctggtgttcc ccggcgacca taacccgtat aagccgatga
     4681 gcgaaaacac tgtcaaccgg gcgttgcgtc ttatgggcta tgataccaaa actgatgtct
     4741 gtgggcatgg attccggaca atggcgtgta gcgcattagt ggaaactgaa ctctggtcac
     4801 gggataccgt ggagcggcag atgagtcatc aggagcgcaa cagcgttcgg gcagcgtata
     4861 tccaccgggc tgaacatctc gacgcccgta cagccatgat gcagtggtgg tcggattatc
     4921 tggatgtaag tcgcgagggg tacgttgcgc cgtatattta tgcgcggcgg cataaggcgg
     4981 cctgagacag tggctatata atttgttaaa aatgaaaaag cagatcctga ttgaatccgg
     5041 atctgctttt tacattaggc aatcgctgcc agacaatctg caccagaaac gccataactg
     5101 caccagttca gtaccaccgc ttacctgcgc cagtctgccg tcgcctgtct gtgtcaggac
     5161 tgcgccagat cttatcgaat aaacatcaaa ctgcgccagt gttacggccc tttcagccct
     5221 ttggcctttt ccggtctggc gctgttaccc ttagccgccc cttacccttt ccggcctttt
     5281 tcctttgctg gcgcaatcgc cagaaaaatc gtcaagctgc accagaagcc tgccaatagg
     5341 tcataccgtt cagaagcatc gttattgaga ggaaaccgta caaaaaattt ctacgtagaa
     5401 attttttcgg tggataacga tgaataaaga gtattttata atgtgatggc tatcaaaaat
     5461 cgaccatcca aaaataattc attgttcatt gatttataat gaataaccat gaatttaata
     5521 gtcgatgatt attgttttta tcagtattga taggaaagta tacaaaaaat tttggcccca
     5581 aaattttttg tacgaaaacc gatgaataac gcgaaattaa taggaaacca tccaaaatta
     5641 tttggggcaa ataattttgc ccgaaaaccg atggataact cgatcttttc aaagcaacgg
     5701 ctatcaatca gtatcctttc ttccgtaaac ctgcgccaca taccgcccgc gcccgtcgcc
     5761 gtgtcccaaa tccatcgccg tcattgccag cgcttctttc tcactgaatc cctgcgccag
     5821 ataatgacga atggcatcct gcgcccacgc atagcgcaac gagtgcgggg aatatgtgcc
     5881 agttaacccg agacgtgacg cctggctgtg ccagtatttc atcgcgcttt tcaggtcggg
     5941 tttatctatc agcctgccgt ggcgatcttc tgccacaacc aacgcattat ccagcgcttt
     6001 tctgatagcg acagcatcca gaataacggt ttctcgcgga cgcccgcctt tggtgccgaa
     6061 caccacggtt aaacggggct caccccgctc cagcgattgc ttccacgttc ttaatgactg
     6121 cgcactttgc accacctcct gcgaacgtaa tcccatcagc cttgagagtt ccagcgccgc
     6181 tgccat
//
LOCUS       DADLFA010000204         6101 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000204 DADLFA010000000
VERSION     DADLFA010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6101
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.206"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(23..604)
                     /locus_tag="JQH65_003600"
     CDS             complement(23..604)
                     /locus_tag="JQH65_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6140879.1"
                     /translation="MYYPVTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNA
                     HILEHGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGGCFKKEYSIVSHMLVTD
                     TITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLF
                     QGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(651..4508)
                     /gene="sigA"
                     /locus_tag="JQH65_003601"
     CDS             complement(651..4508)
                     /gene="sigA"
                     /locus_tag="JQH65_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HAY6140880.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFISSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGELTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSSAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEVGAWARI
                     MSGAGSASGGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(4869..5906)
                     /locus_tag="JQH65_003602"
     CDS             complement(4869..5906)
                     /locus_tag="JQH65_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001363149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HAY6140881.1"
                     /translation="MHRGFCVAHEPSILLPSVGAQLSNPDNITDNADRISDEWKAFDR
                     AIESNHGVPEAAARLKEHLRDFRNTHAAAQLGVSAVAALPGDRLSAALMVKSHGTVSV
                     DGKVSDADLTYLEEVANSTGQEVDKSRLTSQAFARAALITDVGIALATELETAGQKWS
                     LGFPPKFQRVDLFNYNVLVRNYDSSAFKGDRYHNTKNGINADIGASTNLDDNWTLGLV
                     AQNLIPRSIETKEVNGITETFRIRPQVTAGVSWHNAMFTTAFDVDLTPASGFTSDSNR
                     QFAAIGAEFNAWKWAQLRAGYRQNLAGNDGSAFTAGVGISPFDVVHLDVAGLIGTDNT
                     YGAVAQFQFTF"
     gene            5858..6101
                     /locus_tag="JQH65_003603"
                     /pseudo
     CDS             5858..6101
                     /locus_tag="JQH65_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1520 a         1360 c         1367 g         1854 t
ORIGIN      
        1 ggctaaaata ccgggacaga gatcaggcgt gattaatctg cagcattttt aataacagaa
       61 gtactgagct tactttacct ttgatgatgt aatagttatt tccgtcggta ctcatttttc
      121 cctgaaataa tgtcacggat gtttcttcct gctgatcctg caggcgcagt cgtaaggtca
      181 tatttttaat gagctgagat ccgggtggca aggggtattt gctgtcagat actgggcgac
      241 tgagtctgat gtggcgcgta tacagcccgt tttcgtacag ggtggcctct gttatggtgt
      301 cagtgaccag catgtgggag acaatgctgt attctttttt aaagcaccca ccagaaacgg
      361 tgattgtaat gataatggtc ttctcgatcg agatgtaaat acccgtttcg ttatactgtc
      421 cgtatcggaa ccatgagaac tcagttgcat tccagccatg ctccagaata tgtgcatttt
      481 tttgcctgat agtttctttt tttattcgct catgtttaag gcataacagg cagataaata
      541 atgtcagaaa aagaaagcta ataatgagag caagagcgat atagtctgtc acagggtaat
      601 acatattctc tccggtagaa gaagggccgc aaacgcggcc cgggctgtta tcagaaagag
      661 taacggaagt tggcgttgat cgcgttatcc acgttgtatt taccaaatgc cgatttctca
      721 aactcaagac caaagcgaag attatcgcga atttcggcgt tgagaccaac attcatgaga
      781 atacgaccgt ctttttcacc tttgatacgt ttctcaccgg acgcatcacg cagtacggtt
      841 tcaccgttgg caaacaggtc aaactggtag ccaaggccgg cgcgggctgt gactttccag
      901 tccttaccgg agaaggattt acccacatca acaccggtac gcccaatcag cggattaaag
      961 tccttatcct tcatggtcac gctcattccg cgatcctgcc agtcgaaccg tttacctgat
     1021 acggccccat aaaccagttc tgcctgcggc tcaatccagg agtcttctgt tacatgatag
     1081 cggtagcctg cttcagcacc cacatacaag gaatgagaac tgtagtcttt tgttccgagc
     1141 ccagcaaagg tcgccgagta ctcattatca tggtgaacat acttactaat caggtcgata
     1201 taggcaccag agtcaaatat agcggaagca tacagacctg ccccgacgga cttcgtcttg
     1261 ccactgaatg cattactgct ggcatgactg tcggtatacg tcatagtcag accagtgaaa
     1321 aggtcaagtc catccagctc atgtttttta tccacaccaa tctgcacatg ggtgtagttg
     1381 tcactgtatc caccagaagc tgaacctgct ccgctcatga tgcgggccca tgcaccgacc
     1441 tcgccattgg tgtcacgcag atcccccatt cgtttattca ggttgttgac ttctgtcagg
     1501 aaggctttgt aattaccgga catatacgat tttgctgcac tggaagatgc tgtattttca
     1561 gccaccttat atccggtcag ggtccacgtg gtatgttcct gatcctcttt cgtgctgatc
     1621 acaggagata tgttgctgaa acctatggcc tgagtactgg ctttaaatat ctctttattg
     1681 cttccttttg gggctgttat caggtcaacg tttaatcctg agttattatt tcccactttc
     1741 tgtatatcga ccgacagact gttattgctt ccggttaact gatttttaac aatgagttta
     1801 tccgattgat ttacagactg gctcagatca gcccgcataa tcacagcagt attagtggca
     1861 tccagtgtgt ttactgtcac agtatggaaa cccgaattct ctatgttact aaacaacaaa
     1921 agactgtcgc gggtttttaa tgattgcagg gctgactgtc cgtttactct ccataaggtg
     1981 ttatacatgc tggctgaggc agcaggagca tcaatacctc cggtataaga agtatcaaag
     2041 ccacccagca aacctgtagc cagcggtgtg tgtgtggttt tgtccaggtt atcttcttta
     2101 tactggtcaa caccaaacag aatagatgct tttttgtctg attttatatt accattgacc
     2161 caggcataat tttgagcctt taacgtggca ccatcttctg tcatattata tccatctgac
     2221 atatagattg ccgggctgta tttattattt tctgtagggg tgcctgttaa tgtcagggtt
     2281 ccggccagtt caatagcttt atctacatac atttttccct gggcggtaac agtagctcct
     2341 ttgttgataa tcaggtctgt attaattaag gataatgcac cagtcatagt aacagttgat
     2401 ttatcaccaa cagttatttt gctgttttga gcactcagac tgccgttgaa actactttcg
     2461 cctgctgaat ccccggagcg aagctgttgt ctgaatccaa aaccttcgcc agtaatgttt
     2521 cttcctgaga acatgtcaat gtatgcagtt ttatttccca gaacaatttg agagttttta
     2581 gcctctatgt gtccttttac attagcatcc cggcctatac tcagggttgc gttttctaaa
     2641 tttaatttcc ggaaattaaa tttacgactt tcccagtcgg gctgggaaaa agaggctatc
     2701 tgattgttgg acttatattc cgtattgtaa cgtttattta cagtgctttc ctggtccttt
     2761 atggcagcag aatagtcttt ttgacataaa aaaggaattg gacagttgtt attgccttct
     2821 ttaaatatgg catgttctgt cgcatgtcct tgtaatgtta actgtgcatt ccggacagag
     2881 atatcgtttt tgatatcgac tgagccatca aagatcagat tggcattatt gttatgttgc
     2941 tgggtagtat tgataatatg atttatgctt atattacctg atacattccc atggtacata
     3001 tatgcatcag tattagtaag tgataatgtt gattgcttca cgtttgagtt cgttacggtt
     3061 gttcccgcat ctgttgctgc gattttctga aatgactggt catagccgtt tagatccagt
     3121 gttccgccat ttctggtaaa atagatttcg ccattaccgc tttcgcttaa agcgtctttg
     3181 gcatttattt ttaccgtacc tttacctccg gccatgtaaa ttttattgaa ggctttttca
     3241 gcactaagga tgacagtccc attacctatt ttaaggttat ttccctgtgc tatttttaca
     3301 tccagagtac cactacctat tttatgcaga ttatctccgg caacgccctt aacattccat
     3361 tcaacggtgg tattattatc aatatctatc ccggcacctt tataagactt atcttttccg
     3421 tacactttat atgtctggtt cttatcgaat ataagcccgc caatgccaag atcggtatcc
     3481 tgcttgagcg ttaattcccc tcctcctgag acagacaggt ccttatcttt tataatcgat
     3541 gataactctt gttttttacc atccagctgc atagtgtttg tgaaattctt ttttaaattg
     3601 tttaccagaa tattattaaa taacgagtag tttgtccagg ctatattggt actactgata
     3661 aaaccaactg tactggtaac accatggaaa acccattttt gttgttcttt atcatataga
     3721 taagaaccag aaccactatc gccaaaaatt ggtgagtttt taaaaggggt tctgttggta
     3781 taaatgtaac tcttgttttt ccagtccaga ttaaaaagac ttgcgcttaa caagtcggga
     3841 ttatagtttt gtccaaacag atattgtttg ccgtttaata tcgttgaggt tgttccggca
     3901 cctgctctga agccaattat ttgttcctta ccgttgtagt ttacgccata tcttttcatc
     3961 gcatcttcgg gagaaagtga gaagtcggca ctctccgtaa cccctgttgt ttccacgacg
     4021 aatttattca gacgatgaac cgaaaagtcg ccagatgata ctctgttact tgctttatat
     4081 gctgtctgat cccatgactg tgttgttatt gcatgatgct gtgtaccgtt atgagtcgca
     4141 gtaacactgt acgcacctcc aattgatgtt gttgcacctt tgttggaaac agcagaaaaa
     4201 tcaggtacag gtagttctgg aaaatgaaat atctttccat ctttcatcat taattgaaca
     4261 tttgttgcac ccggtctgaa ctctcctctg ttttgagcaa ggtcaagata atcgcgggtc
     4321 catatattat ttatatccag cattgctgca aatgctgttt caccataact tccatatatt
     4381 gaggataaaa taataccgag gataactttc tttcttgatg taccgacact aactttccgg
     4441 gtcagttcag aaacagcaac taatccacct gtaatatgac tatatttcag tgaataaatt
     4501 ttattcatac gtgaactccg atatttctga gttgtggtta tgggatagca tttaattaca
     4561 acggggaact atcagtcatg gtacgtaact gataagggta taatgataat cactgtcagt
     4621 tatatttgta tgacagatgt aacaaaatga aaaaaaagat aagcacaagt gattgattgg
     4681 tcagatattg atcacctggt gtttgtattt tgacctttgc gatcaaaatt tgatctttgt
     4741 tgtttccggt caatccttta caggttttct gtattatccg ggggagttaa gtctgtcgat
     4801 gtccgacaga cttttgatga tggaaacaac atgcagcgat aatgtgcatg ctgttttgca
     4861 ggtggaactc agaaggtgaa ctggaactgt gcgaccgcac cgtaagtgtt gtccgtgccg
     4921 atcaggcctg caacatcaag gtgaaccaca tcaaacggtg agatccccac cccggccgtg
     4981 aatgcactgc cgtcgttacc ggccagattc tgacggtaac cggcgcgcag ctgtgcccat
     5041 ttccaggcat taaattctgc gccaatggcg gcaaactgac ggttgctgtc ggaggtgaaa
     5101 ccgctggccg gggtcagatc cacatcaaat gcggtggtga acatcgcgtt gtgccaggag
     5161 acaccggccg tcacctgcgg gcggatcctg aaggtttccg tgataccgtt cacttctttt
     5221 gtctcaatac tacgggggat caggttctgt gcgaccagtc ccagcgtcca gttgtcatcc
     5281 aggttcgtac tggcaccgat gtcggcgttg atgccatttt tcgtgttgtg gtagcggtcg
     5341 cctttgaagg cgctgctgtc ataatttctg accagcacgt tgtagttgaa caggtcgacg
     5401 cgctggaatt tgggggggaa gcccagagac catttctgcc cggccgtttc cagttccgta
     5461 gccagagcaa tacccacatc agtgatcagt gccgcgcggg caaaggcctg tgatgtcagg
     5521 cggcttttgt ccacctcctg cccggtgctg tttgccacct cttccaggta agtgaggtca
     5581 gcatcagaga ctttaccgtc gacactgacg gtgccgtgtg atttaaccat cagtgcggca
     5641 gagaggcgat caccgggaag ggccgccaca gcagaaacgc ccagctgtgc ggcggcatgt
     5701 gtgttacgga agtcccggag gtgttctttc aggcgtgcgg cagcctcagg aacaccgtgg
     5761 ttactttcga tggcgcggtc gaaggccttc cactcatcac tgatccggtc ggcgttgtcg
     5821 gtgatattat ccggattgga gagctgcgcg ccgacggacg gaagcaggat gctgggctca
     5881 tgcgcgacgc aaaatccacg atgtatatat cagtactaaa agcgcgacgg cggaagaagc
     5941 tctgaaacta atcggtgaac tgtacgccat agagcacgat atacgcgggt taccggtgtc
     6001 tgaacgcctg gcggtcaggc aaatgcagag tagaccgctc ctgacttccc tgtataagct
     6061 gatgcaggag aaagaacaca cgttatcgaa gaaatgtcgt c
//
LOCUS       DADLFA010000205         6053 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000205 DADLFA010000000
VERSION     DADLFA010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6053
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.207"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..2014
                     /locus_tag="JQH65_003604"
     CDS             <1..2014
                     /locus_tag="JQH65_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HAY6140882.1"
                     /translation="RGDYSALISEFKTFWHSYQREWQLYMLAALPIEKTAQAWNVLSK
                     EPHVGVEFVMTHLQLAGLQGFIHSFSRYPQEALPVAQYFAAIELAPLIARAFNKLKTL
                     RENARTWLLKYPEHAITGLLPSALDKAGEAQDNARAALRMLTENGHQPLLQEIARRYN
                     QPEVTDAVNALLALDPLDNHPTKIPTLPAFYQPSLWTRPVLKANAQSLPDSALLHLGE
                     MLRFPQEEALYPGLLQVKDACTADSLAEFVWDLFTAWLTAGAPSKESWAFTALGVLGN
                     DDTARKLTPLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNGIAQKLKFKALQERA
                     KEKIADIAESRELTVAELEDRLAPDLGLDDNGSLLLDFGPRQFTVSFDETLKPFVRDA
                     SGSRLKDLPKPNKSDDESRSNDAVNRYKLLKKDARIVAAQQVARQESAMCLRRRWSPE
                     NFKLFLVEHPLVRHLTRRLIWGVYSAENQLQACFRVAEDNSYSTADDDLFTLPEGGIS
                     IGIPHVLEISPTDAAAFGQLFADYELLPPFRQLDRNSYALTGAERNASELTRWAGRKC
                     PSGRVMGLANKGWIKGTPQDGGWIGWMIKPLGRWSLVMEIDEGFAVGMSPAELSAEQL
                     LSKLWLWEGKAESYGWGSNSTQEAQFSVIDAITASELINDIEALFE"
     gene            2024..2266
                     /locus_tag="JQH65_003605"
     CDS             2024..2266
                     /locus_tag="JQH65_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HAY6140883.1"
                     /translation="MDKELPWLADNAQLELKYKKSKTPLSHRNWPGEPVSVITESIIQ
                     TLGDELLQKAEKKKRLSGVMKIFHWSGSPPSRRPST"
     gene            2263..5655
                     /locus_tag="JQH65_003606"
     CDS             2263..5655
                     /locus_tag="JQH65_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001440040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HAY6140884.1"
                     /translation="MIGEHKPSVPARTMAALACIAQNDSQQLLDEIVQQEGLEYATEV
                     VIARLFIARCYESDPLVVTLQYQDEDYGYGYRSETYNEFDLRLRKHLSLAEESCWQRC
                     ADKLIAALPGITKVRRPFIALILPEKPEIANELVGLECPRTHFHSKKWLKVVANDPTA
                     VRKLEHYWSQDIFSDREASYMSHENHFGYAACAALLREQGLAAIPRLAMYAHKEDCGS
                     LLVQINHPQVIRTLLLVADKNKPSLQRVAKYHKNFPHATLAALAELLALTEPPARPGY
                     PIIEDKKLPAQQKARDEYWRTLLQTLMASQPQLAEEVMQWLSTQARAVLNSYLSAPPK
                     PVIDSTDNSNLPEILVSLPWRSKKKMTAPRLDLAPLELTPQVYWQPGEQERLAATESA
                     RYFSTESLAQRMEQKSGRVVLQELGFGDDVWLFLNYILPGKLDAARNSLIVQWHYYQG
                     RVEEILNGWNSPEAQLAEQALRSGHIEALINIWENDNYSRYRPEKSVWNLYLLAQLPR
                     EMALTFWLRINEKKHLFAGEDYFLSILGLDALPGLLLAFSHRPKETFPLILNFGATEL
                     ALPVARVWRRFAAQRDLARQWILQWPEHTASALIPLVFTKPSDNSEAALLALRLLYEQ
                     GHGELLQTVANRWQRTDVWSALEQLLKQGPMDIYPARIPKAPDFWHPAMWSRPRLITN
                     NQPVTGDALEIIGEMLRFTQGGRFYSGLEQLKTFCQPQTLAAFAWDLFTAWQQAGAPA
                     KDNWAFLALSLFGDESTARDLTTQILAWPQEGKSARAVSGLNILTLMNNDMALMQLHH
                     VSQRAKSRPLRENAAEFLQVVAENRGLSQEELADRLVPTLGLDDPQALSFDFGPRQFT
                     VRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPEALARLKGLKKDATQVSKNLLPR
                     LEAALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWGVYPANEPRCLLKAFRVAAEGEF
                     CNAQDEPIDLPADALIGIAHPLEMTVEMRSEFAQLFADYEIMPPFRQLSRRTVLLTPD
                     ESTSNSLTRWEGKSATVGQLMGMRYKGWESGYEDAFVYDLGEYRLVLKFSPGFNHYNV
                     DSKALMSFRSLRVYRDNKSVTFAELDVFDLSEALSAPDVIFH"
     gene            5717..>6053
                     /locus_tag="JQH65_003607"
     CDS             5717..>6053
                     /locus_tag="JQH65_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6140885.1"
                     /translation="MIVQKELVAIYDYEVPVPENPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDVDPLINDALIYIRDEFIDERKLRGESPEPVPVIFNCELQNIFNQ
                     LIEYYFIKVG"
BASE COUNT         1551 a         1565 c         1533 g         1404 t
ORIGIN      
        1 gaggggagat tattctgcgc tgatcagcga atttaaaacc ttctggcact cctatcaacg
       61 ggaatggcag ctttacatgt tagccgccct gcctatcgaa aaaacagcgc aggcgtggaa
      121 tgtcctcagt aaagagccac acgtcggtgt cgagtttgtc atgacgcacc tgcaactcgc
      181 tgggttacag ggttttattc actccttttc acgctatcca caagaagctc tacctgtagc
      241 acaatatttt gcggctatcg aattagcgcc actgattgcc cgtgccttca acaaactcaa
      301 aacgctacgc gaaaacgccc gcacctggct gttgaaatac ccggaacatg ccattaccgg
      361 cctgctgcct tcggcgctcg acaaagccgg tgaagcacag gataacgccc gcgctgcctt
      421 gcgtatgctt accgaaaatg gtcatcagcc attactgcaa gaaatcgccc ggcgttataa
      481 ccagccggaa gtaaccgacg cggtgaacgc tctgcttgcg ctcgatccct tagataatca
      541 cccaacaaaa atccccactc ttccggcctt ttatcagcca tcgctctgga cgcgcccggt
      601 attaaaagca aacgcccaat cactgccaga tagtgccctc ctccacctcg gcgaaatgct
      661 ccgcttccct caggaagagg ctctgtatcc gggattattg caggtgaaag atgcctgtac
      721 cgccgactca ctggctgaat ttgtctggga tctgtttacc gcctggctga ctgcgggcgc
      781 gccgtcgaaa gagagttggg cgtttactgc gttaggcgtt ctcggtaacg atgataccgc
      841 tcgcaaactg acgccattaa tccgcgcctg gcctggtgaa tcccagcata aacgcgccac
      901 cgttgggttg gatattctcg ccgctatcgg tagtgatatt gccctgatgc agcttaacgg
      961 catcgcccag aaacttaaat tcaaagcatt acaggagcgg gcaaaagaaa aaattgccga
     1021 cattgccgag agccgcgaac tcacggtggc ggagcttgaa gatcggttag caccggatct
     1081 cggtctggat gataacggtt cgttgctgct ggatttcggc ccacgccagt tcaccgtcag
     1141 ctttgatgaa accttaaaac cgtttgtgcg tgacgcttcc ggcagccgcc tgaaagacct
     1201 gcccaaacca aacaaaagcg atgatgaatc gcggtcgaac gatgcggtta accgctacaa
     1261 attgctgaaa aaagatgcgc gtatcgtcgc cgcccagcag gtagcaaggc aggaatccgc
     1321 catgtgcctg cgtcgccgct ggtcgccaga aaactttaag ctcttcctgg ttgagcatcc
     1381 gttggttcgc cacttaaccc gccgtctgat ttggggcgtt tatagcgccg aaaaccagct
     1441 acaggcgtgc tttagggtgg cggaagataa cagctacagc accgctgacg atgatctttt
     1501 caccctgccg gaaggcggta tctctatcgg cattcctcac gttctggaaa tatcaccgac
     1561 ggatgctgcc gcctttggtc agctttttgc cgactacgaa ctgctaccac cattccgtca
     1621 gcttgaccgt aacagttacg ccctgacagg agccgagcgc aacgccagcg aactgacccg
     1681 ctgggcaggc agaaaatgcc cgagcggtcg ggtcatgggg ctggcgaata aaggctggat
     1741 aaagggcacc ccgcaggatg gaggctggat cggctggatg atcaaacccc tgggccgctg
     1801 gtcgttagtc atggaaatcg atgaaggttt tgcggtaggc atgtcgccag ccgaactcag
     1861 cgctgagcag ctcttaagca agctgtggct atgggaaggc aaagcagaaa gctatggctg
     1921 ggggagtaat tcaacacagg aagcgcagtt ctccgtaatc gatgccatca ccgccagcga
     1981 gctaattaac gatattgaag ccctgtttga ataaggaaag agcatggaca aggaattacc
     2041 gtggctggcg gataacgccc aactggaact gaaatataaa aaaagcaaaa cgccgctcag
     2101 tcatcgcaat tggccgggcg aaccggtgtc tgttatcact gaaagcatca ttcagacact
     2161 gggtgatgaa ttgctacaaa aagctgagaa gaaaaaaaga ttgtctggcg ttatgaaaat
     2221 ttttcactgg agtggcagtc cgccatcacg caggccatca acttgatcgg cgaacacaaa
     2281 ccctcagtcc cggcccggac aatggcggcg ctagcctgta tcgcgcaaaa tgacagccaa
     2341 cagttgctcg acgaaatcgt ccaacaagag gggctggaat atgcgactga ggtggtaatt
     2401 gcacgcctgt ttattgcgcg gtgttatgag agtgatcctc tggtagtgac attgcagtat
     2461 caggacgagg attatggcta tggttatcgc tcagaaacct ataacgaatt cgatctccga
     2521 ctgcgtaagc atctctctct ggcagaggaa agctgctggc agcgttgcgc cgacaaactc
     2581 attgccgcac taccaggaat aaccaaagtt cgccgccctt ttattgcgct gatcctcccg
     2641 gaaaaaccag aaatagccaa tgagttggta ggccttgaat gcccgcgaac tcattttcat
     2701 tctaagaagt ggttaaaagt tgttgctaat gaccccacag cggtgagaaa actcgaacac
     2761 tactggagcc aggatatatt tagcgatcga gaagccagct acatgtcgca tgaaaaccac
     2821 ttcggctacg cggcctgcgc cgcccttttg cgcgaacaag gactggcagc cattccgcgc
     2881 ctcgcgatgt atgcccataa agaagattgc ggcagtctgc tggtacaaat taaccatccg
     2941 caagtcatcc gcaccttgct actggtggct gataaaaaca aacccagcct gcaacgtgta
     3001 gctaaatacc ataaaaactt cccccatgcg acgctcgccg cactggcaga actgctggcg
     3061 ttaacagaac caccagcccg ccctggttat ccaatcatcg aagacaaaaa gctgcctgca
     3121 cagcaaaaag cacgcgatga atactggcgt acgctgttac aaacgctgat ggcatcgcag
     3181 ccacaactgg cagaagaggt gatgcagtgg ttaagtactc aagccagggc agtgctgaat
     3241 agttatttat cggcaccgcc caaaccggtt attgatagta ccgataacag caatctgcct
     3301 gaaatcctcg tttcgctacc gtggcgtagt aagaaaaaaa tgacagctcc acgtcttgat
     3361 ttggcaccgc tcgaattaac tccgcaagtt tactggcaac caggcgaaca agagaggctt
     3421 gccgccactg agtctgcccg ttatttcagc acggaatctc ttgcgcaacg catggaacaa
     3481 aaaagtggac gagttgtatt acaggaactg ggttttgggg atgatgtatg gctgtttctg
     3541 aattatatac tccccggaaa actggatgct gcacgcaatt cactcattgt tcagtggcat
     3601 tactaccagg ggcgggttga agagatcctg aatggctgga actccccgga agcacaatta
     3661 gcagaacagg cgctccgcag cggtcacata gaagcgttaa ttaacatatg ggaaaatgac
     3721 aactactcac gttatcgtcc ggaaaagagt gtctggaacc tgtatttatt ggcacagttg
     3781 ccgcgtgaga tggctttgac cttctggctg cgtatcaatg agaaaaagca tctgttcgcg
     3841 ggtgaggact attttctcag tatcctcgga ttggatgcgc taccaggtct gctgttggct
     3901 ttttcacatc gtccaaaaga aacatttccg ttaattttaa attttggcgc aacagaactg
     3961 gcgctgcctg ttgcccgcgt ctggcgacgt ttcgcggcgc agcgtgatct ggctcgccag
     4021 tggattttac aatggccgga acatacggct agtgcactta tccctcttgt ctttaccaaa
     4081 cccagcgata atagcgaagc cgcattactt gccctgcgtt tactgtacga acagggacat
     4141 ggcgaattgc tacaaaccgt ggcaaaccgc tggcagcgta cagatgtatg gtctgccctg
     4201 gagcagttgc ttaaacaggg tccaatggac atttacccgg cacgcattcc aaaagcccct
     4261 gatttctggc atccggcaat gtggtccagg cctcgactta tcactaataa tcagcctgtt
     4321 accggtgacg ctctggaaat tatcggcgaa atgctgcgct ttacccaggg gggacgtttt
     4381 tatagcgggc tggaacaact gaaaacgttc tgccagccac aaacgctggc cgcttttgcc
     4441 tgggatctct tcactgcctg gcaacaagcg ggggcccccg caaaagacaa ctgggcattt
     4501 ctggcattaa gtctctttgg tgacgaaagc acggcacggg atctgacgac acagatcctc
     4561 gcctggccac aagaaggcaa atctgcccgt gctgtcagtg gtctgaatat acttaccctg
     4621 atgaataatg atatggcgct gatgcagctg catcatgtat cacaacgggc gaaatcacgc
     4681 cccttacgtg aaaacgcagc ggaatttctt caggtggtcg cagaaaatcg cgggctaagc
     4741 caggaagagt tagcggacag attagtccca accctgggcc ttgatgatcc gcaggcgttg
     4801 agttttgatt ttggtccccg gcagtttacc gttcgcttcg atgaaaacct caacccggtt
     4861 atctttgatc agcaaaacgt tcgccagaaa agcgttcccc ggttgcgcgc cgatgacgat
     4921 caactgaaag cgcccgaggc actggcccga ctaaaagggc taaaaaaaga tgctactcag
     4981 gtgagcaaaa acctgctccc gcgtcttgaa gctgccctac gtaccacccg acgctggtcg
     5041 ctggcagatt ttcattctct gtttgttaat catcccttta cccgtctggt tacccagcga
     5101 ttaatatggg gggtttatcc ggcaaatgaa ccgcgttgtt tactcaaagc ctttcgtgtg
     5161 gccgcagagg gggagttctg caatgcgcaa gatgagccaa ttgacctgcc tgcggacgct
     5221 ctgattggca ttgcccaccc gttagaaatg acagtagaaa tgcgcagtga atttgcacag
     5281 ctttttgccg attacgaaat tatgccgcct tttcgccagt tgtcgcgccg cacggtgctg
     5341 ctcacacctg acgagtcaac cagtaacagc ctgactcgct gggaaggtaa atccgctacc
     5401 gttgggcagc ttatgggaat gcgatacaaa ggctgggagt caggttatga ggacgcattt
     5461 gtctatgacc tgggtgagta ccggctggtc cttaagtttt cacccggttt taaccactac
     5521 aatgttgata gcaaagcgct aatgagcttc cgttctcttc gagtgtaccg tgacaataaa
     5581 tccgtcactt ttgccgaact tgatgtgttt gatttgagtg aggcgttaag cgcacctgac
     5641 gtcattttcc attaaaaaca cagcgggcag tgatgcaact gcccgttatc aacacgactt
     5701 accagcgggg aaagcgatga tcgtgcaaaa agagctggtt gctatttacg attatgaggt
     5761 ccctgtacct gaaaatccgt tttccttcag acttgagatc cataaatgct ctgaattatt
     5821 tacaggttcc gtctatcgac tggaacggtt ccggctacgt ccaacatttc atcaacgtga
     5881 tcgagaggat gttgacccgc taataaatga tgcgttgatt tatataagag atgagtttat
     5941 tgatgagcgg aaattacgag gtgaatcacc tgaacctgta ccagtaattt ttaattgtga
     6001 actacagaat atattcaacc aactaataga atattacttt attaaagtgg gaa
//
LOCUS       DADLFA010000206         6051 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000206 DADLFA010000000
VERSION     DADLFA010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6051
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.208"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(95..2197)
                     /locus_tag="JQH65_003608"
     CDS             complement(95..2197)
                     /locus_tag="JQH65_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HAY6140886.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            2439..3500
                     /locus_tag="JQH65_003609"
     CDS             2439..3500
                     /locus_tag="JQH65_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HAY6140887.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(3613..5112)
                     /gene="ansP"
                     /locus_tag="JQH65_003610"
     CDS             complement(3613..5112)
                     /gene="ansP"
                     /locus_tag="JQH65_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HAY6140888.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            5379..5996
                     /locus_tag="JQH65_003611"
     CDS             5379..5996
                     /locus_tag="JQH65_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HAY6140889.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNATNISAIADAVCQLPKLQEVLKRNEII"
BASE COUNT         1610 a         1537 c         1444 g         1460 t
ORIGIN      
        1 gtcttgctga aattatgcaa caaaatattg ctattttata ccagcaatac aatcgttaaa
       61 gtaaatgccc ggcaaatacc gggcatcaag gtaattactc aaatctccac gcaatattca
      121 tgccgacacc ataatttcgt ccgggcgaag gttcgtaata tctcccgttt gactcattga
      181 caatgacaga accaacgtat tctttatcga ataaattatc gacacgacca aataaatcca
      241 cagttaaatt gtggtaattg tatttatacc cggtgaataa gccgacgaga gtataagacg
      301 gcgcttttgc cgtattttca tcatctgcca taatatcgcc catataacgc gcttccgtgc
      361 ctgcatacca accatcttcc ggtacataac ctatcgacgc aaagcccata ttacgggcga
      421 tccctggcat ccgattaccg ttacaatcct gttcattgca aacattgctg cgataggtcg
      481 catcaagcca ggtccaggac gcgtttacgc gaaaatctcc tgcgaaacgt tgatcccatg
      541 ccagttcagc gccttgacga cgggtctttc cggcattttt gtaagtcgta cgcccaccgc
      601 tactgctatc gacaacaatt tcatcatcag tgtcggtctg aaacaatgcg agactaagca
      661 gcccatcacc aatacgcgtt ttactgccga tctcaattgt atcgttggtg gatggtttta
      721 aaccaaagtt cataccgctt tgcccatcag cacgataaga cagctcatta atcgtcggcg
      781 tttcaaagcc tcgcccggct gccagataga tattccaggc atcggtcatt gcatatttta
      841 acgaaccggc aggtagccat ttatgataac tggcatcacc gctgtcatcg ccgttacccg
      901 gagtaacgta atggtcgttg gaatcaaacc acacggagct gtagcgcacg ccagcatcca
      961 gcgacagttt ttcgctcagc tgccactgcg tctgtaaata gggatcgatg ttccacatca
     1021 gattgcgttc gtcgcgacgc aactcacctt tttgcccgta ctccggcatg ccgctattca
     1081 ggcggaagtt attgtagccc ttgcggtttt cactcatgtt ttcgtagttc aggccggtag
     1141 tgaacgtgac cggaacgccc agttcaccac ggtgtgtcca gcggctgtct atcccctggt
     1201 aatggcgttg cagggtaatc acgccgcccg catgtgacgg gttaagttgt ggtgccatgg
     1261 gtattgactg gtactgggtc gtttctcgct ctccggcata catcatcaca ctcatatcat
     1321 cttgcgcgct caggctacgc tcatagcgca acccagcctg agtttgcttg atggtttttc
     1381 gcgtgtcgta ctgttctgca cgaggcgctt gttgtggatt agccttccat tctgctttgg
     1441 ttagcccacc tgggtcatct gctttgatat ccacactatt gaaaatcaga cttaatttgc
     1501 tggcttcatc aatgcgtacg cccagtttgg cattggctaa atttttctgt gcgccactat
     1561 ggtcacgata gccgtgggtc gtaaaacgcg tggttgagac ggtgtaatcg acatcgccag
     1621 gctgtgtgcc gtctcccgtt gcgcccgttg ctttcagccc atagcgccag ctgccaaaac
     1681 tgccgtagta actactggct tcaatggttg gtggctgttg tccggtctgg gtggtgacat
     1741 tcattacccc accagacgcg ttgccataca gggcagagaa ggggccacgc agcacttcca
     1801 cattttgcac actgcttaaa tcgatgttgg atgtttgccc ttgcccgtcg ggcatggtgg
     1861 cgggaatacc gtccacatac aggcgaatac cgcgaatacc gtaagtggag cgggagccaa
     1921 atccgcgaat cgacagctgt aaatcttgcg catagttctg ccggttttgt acctgcaaac
     1981 caggcacgcc ggtcagtgat tcggacaagt taatgcgcgg tgttgccagg cgcatctcct
     2041 cgccatccac cacgcttact gctgctgggg tatccagttc tgaaaccacc tgcggtgcgg
     2101 cactgacaat catagtctgt tcatcagcgg caaaaacaac gggggaaagg acaagcagtg
     2161 cgggcaaaac ggtctgtcgg acggaaaaaa tcttcatgaa aaaagccagg ttaagaatgg
     2221 gaaaacgctg tcatggtaat aaaattgtaa acttatgcaa aacgaaacgg cacgatacgt
     2281 taattaattg agaaaattgt agtcgtaacg gcaagaaatg ctccacattt gagaaaataa
     2341 tgattaccat tcccatttat aacaagagcg tgacgatgat tacgcttagc gaagcattgt
     2401 gaagcagcaa aaatattggt tcatcaaagg gagtcgtcat gcatttacgt catctgtttt
     2461 catcgcgcct gcgtggttca ttactgttag gttcattgct tgttgcttca tcattcagta
     2521 cgcaggccgc agaagaaatg ctgcgtaaag cggtaggtaa aggtgcctac gaaatggctt
     2581 atagccagca agaaaacgcg ctgtggctcg ccacttcgca aagccgcaaa ctggataaag
     2641 gtggcgtggt ttatcgtctt gatccggtca ctctggaagt gacgcaggcg atccataacg
     2701 atctcaagcc gtttggtgcc accatcaata acacgactca gacgttgtgg tttggtaaca
     2761 ccgtaaacag cgcggtcacg gcgatagatg ccaaaacggg cgaagtgaaa ggccgtctgg
     2821 tgctggatga tcgtaagcgc acggaagagg tgcgcccgct gcaaccgcgt gagctggtag
     2881 ctgatgatgc cacgaacacc gtttacatca gtggtattgg taaagagagc gtgatttggg
     2941 tcgttgatgg cgagaatatc aaactgaaaa ccgccatcca gaacaccggt aaaatgagta
     3001 ccggtctggc gcttgatagc aaaggcaaac gtctttacac cactaacgct gacggcgaat
     3061 tgattaccat cgacaccgcc gacaataaaa tcctcagccg taaaaagctg ctggatgacg
     3121 gcaaagagca cttctttatc aacatcagcc ttgataccgc caggcagcgt gcatttatca
     3181 ccgattctaa agcggcagaa gtgttagtgg tcgatacccg taatggcaat attctggcga
     3241 aggttgcggc accggaatca ctggctgtgc tgtttaaccc cgcgcgtaat gaagcctacg
     3301 taacgcatcg tcaggcaggt aaagtcagtg tgattgacgc gaaaagctat aaagtggtga
     3361 aaacgttcga tacgccgact catccaaaca gcctggcgct gtctgccgat ggcaaaacgc
     3421 tgtatgtcag tgtgaaacaa aaatccacta aacagcagga agctacccag ccagacgatg
     3481 tgattcgtat tgcgctgtaa tagatatggc gagggtgaaa acggtgagac ttgcctgcct
     3541 tttatcacgg cgtatccggc actttcgtca gtaatctgaa tttgcccggc acgtagccgg
     3601 gcaattttgc aattaactcg ccgtttcagg cttaaacaca atttcctgtt tttcttcatc
     3661 ttcctcgacg actggcgcag tgctgtgaat ttcagcaacg cgtttgcgca caccaaacca
     3721 gcctataacc agcagaatac cgataatcgg cagcgccgcg atagtataag tcccgttcgg
     3781 gtaatcgaac gccatcagca caaggacact cagtaaaaac agtaatgtca gccaggaagt
     3841 gaagggcgcg ccaggcagtt taaaactgac atccgctgct ttgccttctt taatcgcttt
     3901 acgcaggcgc atctggcaca cgatgataaa cgcccatgaa gcgatgattc ccagcgacgc
     3961 gaagttcaac acaatctcaa atacgcgcga cggcaccaga tagttgagga atacgccgac
     4021 gacatacaca accagtgtcg ctagaatccc ggcatacggc acatgctgac gactcatttt
     4081 cgccataaaa ctcggtgcgg aaccgcccat cgccattgag cgcagaatac gtccggtgca
     4141 gtacagacct gagttcaggc tggagagggc agcggtcagc accacaatgt tcataatgct
     4201 gccgatatat ggcacaccca gtttagagaa aaacgtcacg aacggacttt gccccgcctg
     4261 atacgcgctc cacggcaata acataaccag caacaccacg gagccgacgt aaaacaggcc
     4321 aatacgccaa atcacactgt taatggcttt aggcaccatg gtctgcggat ctttacattc
     4381 tccggcagct gtacccacca tttcaatgga ggcaaaagca aacactacgc cctgaatcaa
     4441 caccagcgca ggcagcaaac cgtgggggaa gaagccgcca ttatcggtga ttaaatgaaa
     4501 gccagtggtg ttgccatcca gcggctgacc actaccgagg aacactgtac ccacgaccaa
     4561 aaaggtcaca atggcgagca ctttaataag cgcaaaccag aactccatct ccgcaaacca
     4621 tttcacaccg atcatattca tggtgccaac gatggtaagt gcagcgagcg caaagaccca
     4681 ctgcggcacg ccgccaaacg caccccagta atgcatatac agagcgacgg cggtaatatc
     4741 aacaatcccc gtcatcgccc agttgatgaa gtacatccag ccagcaacat aagcggcttt
     4801 ctcacccaaa aactcacggg cataagagac aaaactgcca ctggaagggc ggtgtagcac
     4861 cagctcaccc aatgcacgca gaataaaaaa cgaaaacaag ccacaaatta aataaaccag
     4921 tgccagtgct ggacccgcca tttgcagtcg ggctcctgca cctaaaaaca agccggtgcc
     4981 aatcgcgccg ccaatggcga tcatctgtac ctggcgattg cccatcgctt tgtgataccc
     5041 ctcttcgtgg gcattaagcc agcggcgttt cgcggcgtgt tgatctgaag tgtcggtgtc
     5101 gtgtttactc attgctctcc ctgattgctt taatgaaaaa gtcatataag ttgccatgaa
     5161 caatgtttat tcatcctggc gatgatagtt atcaccatta ttctttatgg gtataaaact
     5221 atcggcgcag catcctaccc gttatcgata tacgatgcaa aacatcccct tacaatcctg
     5281 aaggggattc atacaactga cgaaaaaatg acaaatactt ttgctggtta acctgtgtac
     5341 tgtcctacac ttaatcttta aaagattgtg aggggcatat gattaaggtg tatggcgtac
     5401 ccggatgggg ctctacaatc agtgagctga tgctaacact ggctgatatt ccttatcagt
     5461 tcgttgatgt cagtggtttt gaccacgagg gaacttcacg cgagttattg aaaaccctga
     5521 acccgttgtg tcagattccg acactggcgc tggaaaatga cgagattatg acggagacag
     5581 cggcgattgc attgatggtt ctcgatcgtc gcccggacct tgcgccaccg gttgggcgcg
     5641 ccgaacgtca gcagtttcaa cggctattgg tctggctggt tgctaatgtc tatccaacat
     5701 tcactttcgc cgattaccct gagcgttggg ctcctgacgc acctgaacag ttaaagaaga
     5761 atgttattga atatcgtaaa tcgctttata tctggctgaa ttcacaattg accgctgaac
     5821 cttatgcgtt tggtgagcaa ctaacgctgg tggactgtta tctttgcact atgcgcacat
     5881 gggggcctgg gcatgaatgg tttcaggata atgccacgaa tatcagtgcg attgctgatg
     5941 ctgtatgtca gttaccgaaa ttacaagaag tattaaaaag gaatgaaatt atttgagtgg
     6001 gataccagag gattatgtta tttattattc agtaacttta tccacaatga t
//
LOCUS       DADLFA010000207         6049 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000207 DADLFA010000000
VERSION     DADLFA010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6049
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.209"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(24..392)
                     /locus_tag="JQH65_003612"
     CDS             complement(24..392)
                     /locus_tag="JQH65_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6140890.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQITTKMLFMCRE"
     gene            918..1613
                     /gene="minC"
                     /locus_tag="JQH65_003613"
     CDS             918..1613
                     /gene="minC"
                     /locus_tag="JQH65_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HAY6140891.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1637..2449
                     /gene="minD"
                     /locus_tag="JQH65_003614"
     CDS             1637..2449
                     /gene="minD"
                     /locus_tag="JQH65_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HAY6140892.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2453..2719
                     /gene="minE"
                     /locus_tag="JQH65_003615"
     CDS             2453..2719
                     /gene="minE"
                     /locus_tag="JQH65_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HAY6140893.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(3198..3317)
                     /locus_tag="JQH65_003616"
     CDS             complement(3198..3317)
                     /locus_tag="JQH65_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAY6140894.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3278..3463)
                     /locus_tag="JQH65_003617"
     CDS             complement(3278..3463)
                     /locus_tag="JQH65_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6140895.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TAFKKCLFLMRDIIISS"
     gene            3564..3737
                     /locus_tag="JQH65_003618"
     CDS             3564..3737
                     /locus_tag="JQH65_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6140896.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVRATYLIPDI"
     gene            3799..4083
                     /locus_tag="JQH65_003619"
     CDS             3799..4083
                     /locus_tag="JQH65_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HAY6140897.1"
                     /translation="MADMNRTTKGALLGAGVGLLTGNGVNGVLKGAAVGAGVGAVTEK
                     GRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVIGGTGGAILGKMK"
     gene            4087..4422
                     /locus_tag="JQH65_003620"
     CDS             4087..4422
                     /locus_tag="JQH65_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HAY6140898.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4479..6049)
                     /locus_tag="JQH65_003621"
                     /pseudo
     CDS             complement(4479..6049)
                     /locus_tag="JQH65_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         1662 a         1396 c         1384 g         1607 t
ORIGIN      
        1 tagaacataa tgtaaacaag gcatcactca cgacacataa ataacatctt tgtggtgatt
       61 tgattgattt ttccactccg cttaccatcc acaccaaaat aacgatcatc acgacataat
      121 ttttctttaa catcgcaaaa tacaccgtta gcgaatgtaa attcgctggg atcgtagcct
      181 tgtaatgatt gtaagttttc agcggctttt tcgccgagat atttccttgt taactctggg
      241 gaaatgccat ctgagttcac acaaatatac gggtcgcaaa caacgccgca cgccggagat
      301 tgaagtccgc cagcgtcatg tccagacgcc attgatgaga acatcatccc cacacctgac
      361 aatccgatat aaaaaatacg catcatattc atagcacatc ctgataatcc aaccgtacaa
      421 cacttatgct catttcatca tcaatccgta atcaaaaccg ctgacctgga cgagttacga
      481 tgacctgaca gaagtatcag acgtcttctg cggtttctat gattatgata gagcaattct
      541 atggcggata ttaagataac gggtatctgt tctttaatga tgctgcaaaa aatggaaagt
      601 taagaaagcc tggcgtggtg aaaaaataca cttgcctcaa tactggatat ttaaacaggt
      661 ctggagatgc tgtcacgatt agggtgcgga aaaaggaagc gtaaataaga acaatcaaaa
      721 gtacgacggc aatgggttga ttgacaaggg tattttttaa gctatgaatc agcgccattt
      781 atcacagaat agacttttac tctgaataaa tgggagggtg acttgcctca atataatcca
      841 gactataaca tgccttatag tcttcggaac atcatcgcgc gctggcgatg attaatagct
      901 aattgagtaa ggccaggatg tcaaacacgc caatcgagct taaaggcagt agcttcactt
      961 tatctgtggt tcatctgcat gaggcagaac ctaaggttat ccatcaggcg ctggaagaca
     1021 aaatcgctca ggcccccgca tttttaaaac atgcccccgt tgtactcaac gtcagtgcac
     1081 tggaagaccc ggtaaactgg tcagcgatgc ataaggcggt gtcggcaacc ggtttgcggg
     1141 ttattggcgt aagtggctgc aaagatgcgc aacttaaagc cgaaattgaa aagatggggc
     1201 tgcctatcct gacggaagga aaggaaaaag cgccgcgtcc agctcccgca ccacaggctc
     1261 cagcgcaaaa tacaacgccg gtcacaaaaa cgcgtttaat agataccccg gtgcgttccg
     1321 gtcagcgtat ttatgctcca caatgtgatc tgattgttac aagccacgtt agcgctgggg
     1381 ccgaattgat tgccgatggg aacattcatg tctatggcat gatgcgcggt cgtgcgcttg
     1441 caggggcaag tggtgaccgg gaaacgcaaa tattttgtac gaacctgatg gcggaactgg
     1501 tgtccatcgc aggtgaatac tggctgagtg atcaaatccc agcagaattt tatggcaaag
     1561 cggcgcgact gcagttagtc gaaaacgctt tgaccgttca accgttaaat tgatcccttt
     1621 ttaacaagga atttctatgg cacgcattat tgttgttact tcgggcaaag ggggggttgg
     1681 taagacaacc tccagcgcgg ccatcgccac tggtttggcc cagaagggaa agaaaactgt
     1741 cgtgatagat tttgatatcg gcctgcgtaa tctcgacctg attatgggtt gtgaacgccg
     1801 ggtcgtttac gatttcgtca acgtcattca gggcgatgca acgctaaatc aggcgttaat
     1861 taaagataag cgtactgaaa atctctatat tcttccggca tcgcaaacac gcgataaaga
     1921 tgccctcacc cgtgaagggg tcgccaaagt tcttgatgat ctgaaagcga tggattttga
     1981 atttatcgtt tgtgactccc cggcagggat tgaaaccggt gcgttaatgg cactctattt
     2041 tgcagacgaa gccattatta ccaccaaccc ggaagtctcc tcagtacgcg attctgaccg
     2101 tattttaggc attctggcgt cgaaatcacg ccgcgcagaa aatggcgaag agcctattaa
     2161 agagcacctg ctgttaacgc gctataaccc aggccgcgta agcagaggtg acatgctgag
     2221 catggaagat gtgctggaga tcctgcgcat caaactcgtc ggcgtgatcc cagaggatca
     2281 atcagtgttg cgcgcctcta accagggtga accggtcatt ctcgacatta acgccgatgc
     2341 gggtaaagcc tacgcagata ccgtagaacg tctgttggga gaagaacgtc ctttccgctt
     2401 cattgaagaa gagaagaaag gcttcctcaa acgcttgttc ggaggataag ttatggcatt
     2461 actcgatttc tttctctcgc ggaagaaaaa cacagccaac attgcaaaag aacggctgca
     2521 gattattgtt gctgaacgcc gtcgcagcga tgcagaaccg cattatctgc cgcagttgcg
     2581 taaagatatt cttgaggtca tttgtaaata cgtacaaatt gatcctgaga tggtaaccgt
     2641 acagcttgag caaaaagatg gcgatatttc tattcttgag ctgaacgtga ccttaccgga
     2701 agcagaagag ctgaaataag cccgctgtaa aaacgcattt atcttcaagg cagagtcatt
     2761 tctgccttga gtttttcatc cctctcatcc acgttgtggt aaagcggcga gtattcttgc
     2821 tgatactcct cattgctatt tccacctccc cctttacacc ttaaggctgt tcccacgcac
     2881 agaacaaaat gtttatgcct ggttgagtaa ataacctcat tgttagtgtg gatacactgg
     2941 gggatgataa ccccgcaatt tcattattca ttgttctccc ataggatgag gtttcccgcc
     3001 gtaactggcg ggcttttttt gccccagaat tttccctttt agccccctgt aaacgaaaac
     3061 tgcgccgaag cgcagtttaa tcgttgatgg tcagaacgac cagttcacga tatatactgc
     3121 gggtatcgac tgatttcaat tccatgccat tggataaatg atagtagcgt ctaagtgacc
     3181 taagctctgc tgcaacatca gcccgcaggc accagaccag gggattcatc ctgaagagac
     3241 agcgcaggtg tattgtgttc accactcacc aaagacatca tgatgaaatg atgatatccc
     3301 gcataagaaa gaggcatttt ttaaacgcag tgcgctgaag tgtagttgga taaaaaatca
     3361 ctcttttcag ataatactgt cgtacgctta tctttcgaca atgaggccgt cggcaaaata
     3421 aaatgactaa tgtaatcgtt gctgatgaat atcttctgct cacataaaaa tcacacaata
     3481 atattgataa atcagattat cttaccttta caacattgcg ttaacagtca atgacacttc
     3541 ttgaataaaa atattgctaa tcaatgagtt attcctcatt taagattatt ctcattcacg
     3601 taaaaaacat aattcctatc atcacagcca cgctcatttt gaattatctg aataatagcg
     3661 agcgttcttt agttaagcag atcttaattg aagacgaaat tattgtgcgt gcaacttact
     3721 taataccgga catctaagtt gaagaaaaaa atacttgcct tcgggcttat ctcagcattg
     3781 ttctgctcca cgccagcgat ggcggatatg aatcgcacca cgaaaggtgc attgctaggc
     3841 gcaggcgttg gtttacttac tggcaatggt gttaatggcg tactgaaagg tgcagctgtt
     3901 ggcgctggtg ttggtgcagt aacagaaaaa ggccgcgacg gtaaaaatgc gcgtaaaggt
     3961 gcaaaagtag gtgccgccgt tggtgctgtc actggcgttc tgaccggcaa tggcctcgaa
     4021 ggggcgatta aaggtgccgt gattggcggt acgggtggtg caattctggg taaaatgaaa
     4081 taaggaatga tgatgaaaaa atttgcttta ctggcgggcc tgtttgtttt cgcaccaatg
     4141 acctgggcgc aggactacaa tattaaaaac ggtttaccgt cagaaaccta tattacttgc
     4201 gcagaagcca acgaaatggc aaaaactgac agcgcacagg ttgcagaaat tgttgcggta
     4261 atgggtaatg ccagcgttgc cagccgtgat ttaaaaattg agcaatctcc ggaactgagc
     4321 gccaaagtgg tagaaaaact gaatcaggtt tgcgctaaag acccgcaaat gctgttgatc
     4381 acggcaattg atgacaccat gcgtgctatc ggcaaaaaat aatatttcat atgtaccgcg
     4441 tcgatgaata cgacgcggtt ttttagctaa cggcgggatc agaacttata tttcaggccc
     4501 accatcactg cggtgtcatt gtagccatta tcacccagct gcacgcccac attcccccag
     4561 acgctggccg ctggattcag gttgccattt acgccaagtt taatttcccc caaattactc
     4621 gccccatctt gttcgacttt cacaccgttc attgagacag caaaatcttt actgttatgg
     4681 atccagttca tttcaacaaa aggttcaaag tcgtcaccgt gcggaccgct gtttttctcc
     4741 tgagtacgaa taaaggtgcg gaaaccaagt cgggtctgga tgttgttatt tgcgtcattc
     4801 tcaatacgtg agccattggc ttcggtgtgc gcattatgat cgacgcccat ccagatagcc
     4861 tgtgcctgtg gctggaatat ccacgtgtat ttcgccgctt ccagaccaaa ggatttattc
     4921 agggcaaagc cctaacccgc ttccagcgag acggttgcgc ctttcgcact ataggattca
     4981 taggctaatt catccccttt caccgagttt ttaaaccagc tatattgcgc ccaggcgtca
     5041 atgtatgcgc cttttttact gatgtcatcg gcaaaccaag tggcatacag ccctgcgcaa
     5101 tagcctctga cactcccttt cgaacgataa tccgacacgc tggaatgagt tgagttgtag
     5161 tcgcgggcat aaccagccat cactcccaaa cgccaactgt cactgtcggt aaaaccaccg
     5221 gttaacagat cggcccctaa ctgcgagacg tagcgatgcg aggtcgttct caactgtccg
     5281 ttgctgtcac gccaggcatt atgcccgcca atttgacgta gccaaagtcg gcttgaacgc
     5341 tcctgttcag ttacaggatc gatgtagcgc atttcacccg cacggtcgtt cagacgcata
     5401 acaaaaagag tgttagcggc tgccaggttg gcgacgtaac ttccagcttc ggggcgcagg
     5461 acgggggtag gcgtttctgt tggttctgaa tttccctctg agctgttatc ctcctcattc
     5521 actgactgat aacttgtcaa ataccagttt ttgccttgtt gttttagttg atattcgaaa
     5581 gctccagctg caatccttgc atcaccttgt ctgaaggctt ctgctggaga ttcatcgcca
     5641 ttcacagtta tgagttcgat cccgtttagc gtggccgcgc cgcttcctcc agcatttttc
     5701 acatagacgg tagttgaacc cgaagtatta ccctcaacaa taagtctgtc cgtagccgat
     5761 ttatcatcac ctaatacggt gcggatatta agctgaccat tattaccgac ataattacct
     5821 tttacggtta atgtttttcc ttgggtaaat gtttgaccat catcatacga aagattgata
     5881 atactgtctg aattagtcag atgggtaaca acggagtcat ctttcatatt ccagattgta
     5941 ttttctgaaa gcttcagcgt ggttgtgccg ctaacgagtt tagtcgctcc atttagaata
     6001 gagtcggacg aaattatgtt taagtcacct ccagtattat taaccagaa
//
LOCUS       DADLFA010000208         6048 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000208 DADLFA010000000
VERSION     DADLFA010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6048
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.210"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            29..922
                     /locus_tag="JQH65_003622"
     CDS             29..922
                     /locus_tag="JQH65_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY6140899.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPGVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRAQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPASRNFRIHLLNALR"
     gene            1057..2277
                     /gene="mlc"
                     /locus_tag="JQH65_003623"
                     /gene_synonym="dgsA"
     CDS             1057..2277
                     /gene="mlc"
                     /locus_tag="JQH65_003623"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HAY6140900.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNLGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPTYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLHG"
     gene            2402..3097
                     /gene="bioD"
                     /locus_tag="JQH65_003624"
     CDS             2402..3097
                     /gene="bioD"
                     /locus_tag="JQH65_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HAY6140901.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(3050..4342)
                     /gene="clcB"
                     /locus_tag="JQH65_003625"
     CDS             complement(3050..4342)
                     /gene="clcB"
                     /locus_tag="JQH65_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HAY6140902.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTEMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(4501..5115)
                     /gene="dmsD"
                     /locus_tag="JQH65_003626"
     CDS             complement(4501..5115)
                     /gene="dmsD"
                     /locus_tag="JQH65_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HAY6140903.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQYEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELSRLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(5158..6012)
                     /locus_tag="JQH65_003627"
     CDS             complement(5158..6012)
                     /locus_tag="JQH65_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HAY6140904.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCASLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
BASE COUNT         1578 a         1552 c         1575 g         1343 t
ORIGIN      
        1 taataatctc aaataagatg ttttaaatat gaatattgaa cttcgtcatc tgcgttactt
       61 tgttgctgtt gcggaagagc tgcatttcgg gcgcgccgct gcccgcctga atatttcgca
      121 accaccgcta agtcagcaga ttcaggcact ggagcaacaa attggtgccc gactgctggc
      181 acgaactaat cgcagtgtat tgctgacggc agcaggaaaa cagtttcttg cagatagtcg
      241 gcaaatcctg tctatggtag atgacgctgc cgctcgcgct gaaagactgc atcagggtga
      301 agcgggggag ttgcgcattg gttttacttc gtccgctcct tttattcggg cggtgtccga
      361 tacgttatcg ctgtttcgcc gtgattatcc cggtgtccat ttacaaaccc gcgaaatgaa
      421 cactcgtgag caaatcgctc cgctcattga aggaacgctg gatatgggat tgctgcgtaa
      481 cacagcgtta ccggagacgc ttgaacacgc agtcatcgtc catgaaccgc ttatggcgat
      541 gatcccgcac gatcatcccc tggcaaataa cccgaatgta acgttggctg aactggcgaa
      601 agaacccttc gtcttttttg atccgcacgt cgggacaggg ctgtatgacg atattctcgg
      661 gctgatgcga cgttaccatt tgacgcccgt catcactcag gaggtgggcg aggcaatgac
      721 catcatcggt ctggtttccg ccggtctggg tgtttcaatt ttgcctgctt catttaaacg
      781 tgctcagctc aacgaaatgc gctgggtgcc gattgctgaa gaggatgcgg tttctgaaat
      841 gtggttggtc tggccgaaac atcatgaaca aagtccagct tcgcgtaact ttcgtattca
      901 tctgctgaat gctctcaggt gagggaaatt tcagcgaaaa agcccgaaaa atgtgctgtt
      961 aatcacatgt ctaagtaaaa atttgacgac acgtattgaa gtgcttcacc atagcctaca
     1021 gattatttcg gagcgcgaaa atatagggag tatgcggtgg ttgctgaaaa ccagcctggg
     1081 cacattgatc aaataaagca gaccaacgcg ggcgcggttt atcgcctgat tgatcagctt
     1141 ggtccagtct cgcgtatcga tctttcccgt ctggcgcagc tggctcctgc cagtatcact
     1201 aaaattgtcc gtgagatgct cgaagcacac ttggtgcaag agctggaaat caaagaagcg
     1261 gggaaccgtg gccgtccggc ggtgggactg gtggttgaaa ctgaagcctg gcactatctt
     1321 tctctgcgca ttagtcgcgg tgagattttc cttgctctgc gcgatctgag cagcaaactg
     1381 gtggtggaag agtcgcagga actggcgtta aaagatgact tgccattgct ggatcgtatc
     1441 atttcccata tcgatcagtt ttttatccgc caccagaaaa aacttgagcg tctaacttcg
     1501 attgccataa ccctgccggg aattattgat acggaaaatg gtattgtaca tcgcatgccg
     1561 ttctacgagg atgtaaaaga gatgccgctc ggcgaggcgc tggagcagca taccggcgta
     1621 ccggtttata ttcagcatga tatcagcgca tggacgatgg cagaggcctt gtttggtgcc
     1681 tcacgcgggg cgcgcgatgt gattcaggtg gttatcgatc acaacgtggg ggcgggcgtc
     1741 attaccgatg gtcatctgct acacgcaggc agcagtagtc tcgtggaaat aggccacaca
     1801 caggtcgacc catatggtaa acgctgttat tgcgggaacc tcggctgcct cgaaaccatc
     1861 gccagtgtgg acagtattct tgagctggca cagctgcgtc tcaatcaatc catgagctcg
     1921 atgttacatg ggcaaccgtt aaccgtggac tcattgtgtc aggcggcatt gcgcggcgat
     1981 ctactggcaa aagacatcat taccggggtg ggcgcgcatg tcgggcgcat tcttgccatc
     2041 atggtgaatt tatttaaccc acaaaaaata ctgattggct caccgttaag taaagcggca
     2101 gatatcctct tccctgtcat ctcggacagc atacgtcagc aggcccttcc tacgtatagt
     2161 cagcacatta gcgttgagag tactcagttt tctaaccagg gcacgatggc aggcgctgcg
     2221 ctggtaaaag acgcgatgta taacggttct ttgttgattc gtctgttgca tggttaacat
     2281 tttttaactg ttctaccaaa atttgcgcta tctcaatttg ggccaggaaa gcataactta
     2341 gactttcaag gttaattatt ttcctagttt atatttgtga agcataacgg tggagttagt
     2401 gatgctgaag cgtttcttta ttaccggtac agacacttct gtagggaaaa cggtggtttc
     2461 ccgcgcattg ctacaagcgt tagcctccca gggaaaaaca gttgcgggat ataaacccgt
     2521 agcgaagggg agcaaagaga cacccgaagg gctgcgtaat aaagatgccc tggtgttgca
     2581 gagtgtttca accatcgaac tgccttatga agcagttaat cctatcgcgt taagcgaaga
     2641 agaaagtagc gtggcgcaca gttgcccaat caattacacc ctcatttcaa acggcctggc
     2701 aaacctgacc gataaagtcg atcatgtcgt ggtagaaggg actggcggct ggcgcagtct
     2761 gatgaatgat ttgcgtccac tctctgaatg ggtggtgcag gaacagttgc cggtgttgat
     2821 ggttgtcggt attcaggaag gttgcattaa ccatgcactg ctaacagctc aggcgattgc
     2881 caacgacggg ctgccgctca ttggctgggt ggctaaccga atcaacccag gactggcgca
     2941 ttatgcggaa atcattgatg tgctgggtaa aaaacttccg gcaccgctca ttggtgaact
     3001 gccttatctg ccgcgcgccg aacagcgaga actggggcaa tacatccgct tagctatgct
     3061 gcgcagtgtg ctggcggtag atagagtcac ggtgtaacgt ccgcgaaatt accgacgcaa
     3121 tgacgcaggc aatcaataaa ccggggagta gctgatactc cccggtcatt tcacatatca
     3181 tcaacgtcga cataatcggc gcgtgcgtgg ttgccgccaa cagtgtcgcc atctcggtca
     3241 atccgagtaa aagcgtaatt tcttcgccat cagggaacca taatcccagg ctacgaccat
     3301 acaacatgcc aatggcaaga ccgataaata gcgtcggtgt aaagacacca ccgggtgcgc
     3361 cggaaccgct actcgccagc acggcacaca gtttacaaag gaagatcccg gcaatgatca
     3421 ttaacagtgg tggggcggtt aaaaaggatt gtacggtgct atagccgttg ccccacactg
     3481 caggtgtgaa cagggaaagc agacccacga tcaacccgcc tagtgccagt tgccagggcg
     3541 gcgcaagttt gagactcaca aatccacgat gacaggcgtt cattaacgtt aacaacagtg
     3601 gtccgcacag acctgccagc acacctgtac tgataatcag cgcatagtca cgagcctgaa
     3661 ccgtcactga gagttgtacg ttgtagagta acgcgtcgct atgattaatc agattgctaa
     3721 ccagcaatgc cacgacggcg gaaataatca ccgggccgag agaggccaac atcatagtgc
     3781 caaacagcac ttcggctata aataaactgc cagcaagcgg ggcacgatag gccgcagcca
     3841 ttcccgccgc ggcgccacag gcgatccata atttccactc ctggcgtggc gtaaaacgtt
     3901 gggcaaaaca ggaggcggca agggcagcta aaagaatcat cgcaccttcg cgaccaattg
     3961 cactgccgct ggttactacc agcagcgagg caagcgattt aaccaggctt gctgcgtaat
     4021 cgaactgtcc atcggtttgc aacgcttcca tgtaatcggt cggcgcatga gggcgttgtt
     4081 gggtaaattt ctgccagccc atcagcaaca aacccgccgc cagtccgccg agcgccggag
     4141 ttagcaaccg tcgccagggg gaaaggtttg tcgctgcatt gaccagactg ccggagtcat
     4201 tattgaggaa caaccactcc agtagcagca tcgcatgacg aaacccggca acggcaaagg
     4261 ccgcgagaat accgacgact gttgcgataa gcagacggcg gaacatagtg cgcagatccg
     4321 gatagatgtg tagatgatgc atgagcagaa atgggcaatg aaacacgggc gactattttg
     4381 cggcaaaaga ggccagccgc aaaagcagtg cgtgctttaa gagataaaaa aacgtgacac
     4441 tgtaacctat tattgccgga ttgcgcgtaa tcgtcaccat ccggcaatat tacggtgatc
     4501 ctatcgaaac agcggtttaa ccgcgacagg aattaacagt tgtgattgcc attgcgccag
     4561 cgttaagcga gacagttcac ccagtgcacg gtagaaaggg tgttctgctt tttcgataaa
     4621 aacatcaaga aaacgtgttg accacggaaa aaggtgccat gccagcaatt cttcgcattc
     4681 tgtctggcga ccattctccg ccaaccacgc agccatcaac agcagtgaac caaaatgatc
     4741 ttccggttcg tattgcttca tttcaaactg aatgcctttc tcgcgcatcc actgacgaag
     4801 tgccaatgtt gaatcgccaa acagcacaga ttcgcgatcc agccagaccg acccccacgg
     4861 cggagacggc agtgcccacg ggccgacaaa caaacgctgc caggcctggg cgtgagtctc
     4921 ttcacactgt gtctgaaaag ccgtcaccag cggcgctaat gacgcttctg gtagaggcca
     4981 ctgagtttcc cagccgtcac tggtgagtac cgcaaccaga ggtgcggctt ccgcactctc
     5041 tggagcgtaa taaaacagcg cgcccaacac gcgcgccgcg acagaaaaat tatcttgctg
     5101 tgaaaaatgg gtcattccta acatcctgaa atgtgcgggt tgccccgcac caatttgtta
     5161 acctgcaata gccattccta ctgtcatgtg caggccataa aagagcacac gcccgatcat
     5221 ctcaccgcca agtatgagga ttagccccag aataagtccg gcaacgtgag gttcacggcg
     5281 acgaatgagc gggcaaagcc agcatccaag acttgcgcat aacaatacca cacgccagac
     5341 ctgtaaggag gcgtaatctg gtaccagtgc gctggcttgt tgcacggatg agtgaataga
     5401 cgccagagac aaaccttgca gcacaatcac cccggcacaa gcgatcagcg ccagaacgct
     5461 aatgatggca aatggtgtgg tattaaatgt aacgcgagct gcccgcagaa ttgcggcagc
     5521 aagtattgga ccgctcaaca ataccgtcag gaagaaagcc agcgtagtgt aaccgttatg
     5581 ccaggttggc acggtgtcga tttgatacac gcaggtcatc atccagacga aaatgacgcc
     5641 gagcgccatg ctgacaagta accagagttt ccccaacgct tgcggcattt taccgatgac
     5701 cgccaccagc caccatagtc caccaacggc aaaaaatatg gaaccggcag caatctcgtt
     5761 actcagacca gaagctccga ttcgattcag cgagttaaac gctcgcagtg gcgatcccag
     5821 gtgcatgaca gaggcaataa acccgatgcc catcaacagc cagaggaaaa acatgccacg
     5881 gacaatacgc tgacggtctg cgtcattttt ggcggcgaac cagccaattc cgctaacaat
     5941 cagcgcgcct actacgcatt gacccaatac ggtaaagata accagtggcc attcatgcca
     6001 tccatttccc attttacact tcctccggat tagccagata accagtgg
//
LOCUS       DADLFA010000209         5977 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000209 DADLFA010000000
VERSION     DADLFA010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5977
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.211"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            252..512
                     /locus_tag="JQH65_003628"
     CDS             252..512
                     /locus_tag="JQH65_003628"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY6140905.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDT"
     gene            519..815
                     /locus_tag="JQH65_003629"
     CDS             519..815
                     /locus_tag="JQH65_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY6140906.1"
                     /translation="MTTTFTGTVSSANSGNYYTIFNTDTGAAFNNVSLAIGDSLGTSY
                     KSGMGIDQKIVKDTSTNKGKAKQTLNFKAWLVGAADAPDLGNFEANTTFQITYL"
     gene            897..3539
                     /gene="yfcU"
                     /locus_tag="JQH65_003630"
     CDS             897..3539
                     /gene="yfcU"
                     /locus_tag="JQH65_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HAY6140907.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQIRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQINYNIMLSHYFNMGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVGT
                     SDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMG
                     GTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNRLPENAEAT
                     QSVVQATLTEGAIGYRKFAVISGQKAMAVLRLSDGSHPPFGAEVKNDNEQTVGLVDDD
                     GNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPITSPAV
                     KPAIQEQTQRVTPTEPPTSISVNQ"
     gene            3559..4311
                     /locus_tag="JQH65_003631"
     CDS             3559..4311
                     /locus_tag="JQH65_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAY6140908.1"
                     /translation="MFNLTNTAKIVVPALALLATAVSFSSHASVTPDRTRLVFNESDK
                     SISVTLRNNDPTLPYLAQSWIEDAKGNKISSPLAVLPPVQRIDAMMNGQVKVQGLPDI
                     NKLPADRESVFYFNVREIPPKSNKPNTLQIALQTRIKLFWRPKALENTSMKSPWQHKV
                     TLIRNGQDFTVNNPTPYYVIISNASAKKGGNPPAGFTPLMMEPKTSAPLNAKMDSVPV
                     LTYINDYGARMPLYFKCTGNSCQVDEEQSRKG"
     gene            4327..4836
                     /locus_tag="JQH65_003632"
     CDS             4327..4836
                     /locus_tag="JQH65_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY6140909.1"
                     /translation="MSHKFVAGAGLLVLAGWGISGLAVAGSDIKQVDLTLRILVDGPP
                     PCSVKGSAVKFGNVIINRIDGTNYQQDAKYTLNCGNKVSDDLRMQLKGNTAVINGETV
                     LSTDIAGLGVRIENAADNSLFAVGENNWTPFNVNTQPQLKAVPVKQSGVQLTPSEFNA
                     TMTMVVDYQ"
     gene            4833..5321
                     /locus_tag="JQH65_003633"
     CDS             4833..5321
                     /locus_tag="JQH65_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY6140910.1"
                     /translation="MKKFLLLLLCAGCGAVLAAEDDITFHGTLMSPPACSISGGQTIE
                     VQFRDLIIDSIDGNYGRQKVDYELSCDSDVRDPEWNMTLSWTGTATSYNDSAIETDVP
                     GFGIELQHDGQRFKLNEPLSINATDFSQKPKLEAVPVKAADAVLTDTTFSAYATLRVD
                     YQ"
     gene            5318..5857
                     /locus_tag="JQH65_003634"
                     /pseudo
     CDS             5318..5857
                     /locus_tag="JQH65_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416836.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
BASE COUNT         1545 a         1460 c         1591 g         1381 t
ORIGIN      
        1 atgtccttaa gataatcttt cttctgtcaa tatattaatt aaatctacat ttactcttta
       61 gtaaaattaa attgcatcag gggtgtggaa acatcactat gcggccccac accaacgaat
      121 acaattttct acgacctgtt ctttttggat cgcatgccgt attcaatggc tgggtataaa
      181 cattggctat tgactgaatc aatgtattgc aatgtttata tttttaatta tttatagttt
      241 aaggacaaat aatgagtaag tttgttaaaa cagctattgc tgcggcaatg gtgatgggcg
      301 tgttcacctc tacggcaaca atcgctgcgg gtaacaacgg tactgcacgt ttttacggca
      361 ccattgaaga ctcagtgtgt tctatcgttc cggacgatca caaactggaa gttgatatgg
      421 gtgatatcgg tgccgaaaaa ctgaaaaata acggcaccac cacgccgaaa agtttccaga
      481 ttcgtctgca agattgcgta tttgatactt aggaaacaat gaccactacc tttactggta
      541 ccgtttcttc tgcaaatagc ggcaattatt acaccatttt caataccgat accggtgcgg
      601 catttaacaa tgttagcctg gcgatcggtg actctctggg tacctcttac aaaagcggca
      661 tgggtattga ccagaaaata gtgaaagaca cttctactaa taaaggcaaa gcgaagcaga
      721 cactgaactt taaagcctgg ctggtcggcg cagctgatgc gccagatctg ggtaatttcg
      781 aagcaaatac caccttccag attacttacc tgtaaggcat acccccatag cgacactgct
      841 atgggggatt taaatggatt ttgaccgaac aagaataaaa acggatagaa acgtgtatgc
      901 ctgaccattc tctttttcga ttacggattc ttccgtggtg cattgcgctg gcaatgtcag
      961 ggagttatag cagtgtctgg gctgaagacg acattcagtt tgattcccgt tttctggaat
     1021 taaaaggcga cacaaaaatt gatctgaagc gtttttccag ccagggatat gttgagcccg
     1081 gaaaatacaa tttacaggtt caactaaata aacagccatt ggcggaagag tacgatattt
     1141 actggtatgc tggtgaagat gacgcgagca aaagctatgc ttgtctgaca ccggaactgg
     1201 tagcgcagtt tggtttaaaa gaagacgtgg cgaaaaatct gcaatggagc cacgatgcta
     1261 aatgcctgaa atccggtcaa ctggaaggca tggaaattaa ggctgattta agccagtccg
     1321 cattagtcat ttcactgcca caggcttacc tcgaatatac ttatcccgac tgggatccgc
     1381 cttcacgttg ggatgacggc atctccggga tcgtcgcgga ctacagcatc aacgcacaaa
     1441 cccggcacga agaaaatggc ggtgatgata gtaacgagat cagcggcaac gggacggtcg
     1501 gggttaacct ggggccgtgg cgtatgcgtg ctgactggca gactaactat caacatactc
     1561 gcagtaatga tgacgatgaa ttcagcggcg atgaaactca aaaaaaatgg gagtggagtc
     1621 gctactatgc ctggcgggcg ttaccatcat taaaagccaa actggcgctg ggcgaggatt
     1681 acctcagatc cgatattttt gatggtttta actatgttgg tggtagtgtc agtactgacg
     1741 atcaaatgtt gcctcccaat ctgcgcggct acgcgccaga catttccggc gtggcacaca
     1801 ccacagcaaa agtgaccgtc agccagatgg ggcgtgtgat ttacgaaacg caggttccgg
     1861 ctggaccgtt tcgtattcag gatcttggtg attctgtctc cggtacgttg catattcgca
     1921 ttgaagaaca gaacggccag gtgcaggaat atgacatcag caccgcctcg atgccatacc
     1981 tcactcgtcc aggtcagatt cgctataaga tcatgatggg ccgtccgcaa gagtggggac
     2041 accatgtcga gggtgaattt ttttctggtg ctgaagcttc ctgggggatc gctaacggct
     2101 ggtcgttata tggcggcgca ctgggagatg aaaactatca gtctgcggcg cttggcgtcg
     2161 gtcgcgattt gtctacattc ggcgcggtcg cgtttgatgt tactcactcg cacaccaaac
     2221 tggataaaga caccgcttat ggcaaaggtt cgctggacgg taactccttc cgtgtgagtt
     2281 attccaaaga ctttgaccag ctcaacagcc gcgttacttt tgctggatat cgcttctcgg
     2341 aagagaactt tatgaccatg agcgagtatc tggatgccag tgacagcgga atggtacgca
     2401 cgggcaacga caaagagatg tacaccgcca cttataacca gaacttccgc gatgcgggtg
     2461 tttcggttta tctcaactat acccgccata cctactggga tcgcgaggag cagataaact
     2521 acaacatcat gctctcgcac tatttcaata tgggcagtat tcgtaatgtc agcatctcga
     2581 tgactggcta ccgttacgag tatgacaacc aggccgacaa aggcatgtac atttcgctca
     2641 gtatgccgtg gggcgacaac agtaccgtta gctataacgg taactatggc agtgggacgg
     2701 acagcagtca ggtcggttat ttcagccgtg tcgatgacgc gactcactat cagttgaacg
     2761 tcggcaccag tgacaaacac accagcgttg acggctatta cagccatgat ggttcgctgg
     2821 cgcaggttga cctcagtgcg aactaccatg aagggcaata cacctctgcg ggcttgtcgt
     2881 tacagggcgg cgcaacgctt actgcccacg gtggcgcact tcaccgtacc cagaatatgg
     2941 gcgggacacg cttgttgatt gatgccgatg gcgttgccga tgttccggtg gaaggtaacg
     3001 gggctgctgt ttataccaat atgtttggta aagccgtcgt ttctgacgtc aataactatt
     3061 accgcaatca ggcgtatatc gacctcaaca gattgcctga aaacgctgaa gcaacccagt
     3121 cggtggtgca agccacgcta actgaaggtg ccattggcta ccgtaaattt gctgtcatta
     3181 gcggacaaaa agcgatggcg gtgctgcgcc tgagcgacgg cagccatcct ccgtttggcg
     3241 cagaagtaaa aaatgataac gagcagacag tgggccttgt cgatgatgac ggcaatgttt
     3301 atctggctgg ggtgaaacct ggcgaacaca tgagtgtgtt ctggagtggt gttgcgcatt
     3361 gcgatatcaa cctgccggac ccgctgcctg ccgatctgtt taacggcttg ttactaccat
     3421 gccagcataa aggcaatgta gcacctatca cttcgccggc ggtcaaaccg gcgattcagg
     3481 aacagacaca gcgggtgacg ccaacggaac ctccgacttc aatttcagta aaccagtaac
     3541 gtgattaagg aatgatccat gtttaatctg actaacactg caaaaatcgt tgtcccggca
     3601 ctggcgctgc tggcgacagc ggtcagtttc tccagccacg cttcggtgac gcccgacagg
     3661 acacggctgg tgtttaacga aagcgacaag tccatcagcg tcacgctgcg caacaacgat
     3721 cccacactgc cttatctggc gcagagctgg atagaggatg caaagggcaa caaaatcagc
     3781 tctccgcttg cggtgcttcc gccagtgcag cgcatcgatg cgatgatgaa cggccaggtg
     3841 aaggtgcagg ggctgccgga tatcaacaaa ctgccggccg accgcgagag cgtgttctat
     3901 ttcaacgtgc gcgaaattcc gccgaaatca aataaaccga acacactgca gattgcgctg
     3961 cagacgcgta tcaaactgtt ctggcgaccg aaggcgctgg agaacaccag catgaaaagt
     4021 ccgtggcagc ataaggtgac gctcatccgt aacggccagg acttcaccgt caataacccg
     4081 acgccgtact acgtgatcat cagcaacgcc agcgctaaaa aaggcggcaa cccgccggcg
     4141 ggattcacac cgctgatgat ggagccgaaa acctccgcac cgctgaatgc gaagatggac
     4201 agcgtgccgg tgctgactta catcaacgac tacggcgcgc ggatgccgct gtacttcaaa
     4261 tgcaccggca acagctgcca ggtggatgaa gagcagagcc gcaaaggctg atgccgggag
     4321 ggacttatga gtcacaagtt tgtagcgggc gcagggttgc tggtgctggc cggatggggc
     4381 atcagcggtt tggctgttgc tggcagtgat atcaaacagg tggatctgac gctgcgaatc
     4441 ctagtcgatg gaccgccgcc ttgctccgtg aagggaagcg ctgtgaaatt tggcaatgtg
     4501 ataatcaaca ggatcgatgg taccaattac caacaggatg cgaaatacac cctcaactgc
     4561 ggtaataaag tcagcgatga cttacggatg cagctcaaag gcaataccgc cgtgatcaac
     4621 ggagaaacgg ttctcagcac ggatatcgcc ggcctggggg tgcggataga aaacgccgcc
     4681 gacaactccc tgtttgcagt tggtgaaaat aactggacgc cttttaatgt caacactcag
     4741 cctcagctga aagcggtgcc ggtaaaacag agcggggtgc aactgactcc gtcggagttt
     4801 aacgccacga tgaccatggt ggtggattac caatgaaaaa attcctgctc cttttactgt
     4861 gcgctggttg cggggctgtg ctggcggcgg aagatgacat cacattccac ggtacgctga
     4921 tgtcgccgcc cgcctgtagc atcagcggcg ggcaaaccat cgaagtgcaa tttcgcgatc
     4981 tcattatcga cagcattgat ggcaattatg gtcgtcagaa agtggactac gaactgagct
     5041 gcgactcgga tgtccgcgat cccgagtgga atatgacact ctcctggacg gggactgcca
     5101 cctcctacaa cgactctgct attgagacgg atgtaccggg cttcggaatt gagcttcagc
     5161 atgacggtca gcgttttaag ctcaacgagc cgctttccat taacgccacc gattttagcc
     5221 agaagccaaa gctggaggcg gtaccggtca aagcggctga tgcggtgctg acggacacga
     5281 ccttttctgc ttacgcaacc ttacgggtgg attaccaatg aaaacagcac acatttattc
     5341 acccggttat gcgctgttag caggcctgct gctgaccgcc agcacaggag cacaggcagt
     5401 cgacaacaac ctgcattttt acggcaacct gctgagtaaa tcctgcacgc tggtggtgga
     5461 tggtgccaac ctggcagaaa tccattttcc caccgtcagc aaccgggatt tgatggtatc
     5521 tgggcagtcc gcgcatctgc cggttgtgtt taagctgaaa gactgcaaag ggccggcggg
     5581 atacaacgtc aaagtcacgc tgagcggcac cgaagacagc ggtcagccgg gttttctggc
     5641 agtcgatgac acctccaccg cgcagggcgt ggggataggg atggaaacgc tcgacggcaa
     5701 gcgggtggat attaacaaca ccaccggggc gacctttgcg cttaccaacg gcaacaatga
     5761 tattaatttc cgggcctgga tctaggcgaa aagcggtcgt gatgtaacca tcggtgattt
     5821 caccgccagc ctgacagcaa cttttgagta tatttaaaat gaaaatcctt cgtcatgttt
     5881 tatgtctttt attgctgtta atgttcaata catgcggcca ggcggcgacg aaagttggtg
     5941 agttatatgt gccagctggt gccagttcac ctgcgac
//
LOCUS       DADLFA010000210         5811 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000210 DADLFA010000000
VERSION     DADLFA010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5811
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.212"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..853
                     /gene="pdeC"
                     /locus_tag="JQH65_003635"
     CDS             <1..853
                     /gene="pdeC"
                     /locus_tag="JQH65_003635"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HAY6140911.1"
                     /translation="ALPAGILGSLVLLLLWLRIRQNYLSPKRKLQRALEKHQLCLYYQ
                     PIIDIKTEKCIGAEALLRWPSEQEQVMNPAEFIPLAEKEGMIEQVTDYVIDNVFRDLG
                     DYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKFEVTEHAFLDVEKM
                     TPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVETLTTHKTSHLIAE
                     HIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMPPQVFMQWMEQLPA
                     RELTRGQ"
     gene            complement(856..1179)
                     /gene="soxS"
                     /locus_tag="JQH65_003636"
     CDS             complement(856..1179)
                     /gene="soxS"
                     /locus_tag="JQH65_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HAY6140912.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSPQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            1265..1729
                     /gene="soxR"
                     /locus_tag="JQH65_003637"
     CDS             1265..1729
                     /gene="soxR"
                     /locus_tag="JQH65_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HAY6140913.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYRRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            2274..3623
                     /locus_tag="JQH65_003638"
     CDS             2274..3623
                     /locus_tag="JQH65_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAY6140914.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFGMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            3775..5424
                     /locus_tag="JQH65_003639"
     CDS             3775..5424
                     /locus_tag="JQH65_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HAY6140915.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(5578..>5811)
                     /locus_tag="JQH65_003640"
     CDS             complement(5578..>5811)
                     /locus_tag="JQH65_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAY6140916.1"
                     /translation="MFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVTLQKFF
                     EEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
BASE COUNT         1257 a         1375 c         1604 g         1575 t
ORIGIN      
        1 tgcgttgccc gccggtattt tggggagtct tgttctgcta ttactctggc tacgtattcg
       61 acaaaactat ttgtctccca aacgcaaatt gcaacgcgct ctagaaaaac atcaactttg
      121 tctttattac cagccaataa tcgatatcaa aacagaaaaa tgtatcggcg ctgaagcgtt
      181 gttacgttgg cctagtgagc aggagcaagt aatgaatccg gcagagttta ttccgctggc
      241 agaaaaggag gggatgatag aacaggtaac tgattatgtt attgataatg tcttccgcga
      301 tctgggcgat tacctggcaa cacatgcaga tcgctatgtt tctattaacc tgtcggcctc
      361 cgattttcat acgtcacggt tgatagcgcg aatcaatcag aaaacagagc aatacgcggt
      421 gcgtccgcag caaattaaat ttgaagtgac tgagcatgca tttcttgatg tcgaaaaaat
      481 gacgccgatt attctggctt tccgccaggc tggttacgaa gtggcaattg atgattttgg
      541 tattggctac tctaacttgc ataaccttaa atcattgaat gtcgatattt tgaaaatcga
      601 taaatcgttt gttgaaacgc tgaccaccca caaaaccagt catttgattg cggaacacat
      661 catcgagctg gcgcacagcc tggggttaaa aacgatcgct gaaggcgtcg aaactgagga
      721 gcaggttaac tggctgcgca aacgcggcgt gcgctattgc cagggatggt tttttgcgaa
      781 ggcgatgccg ccgcaggtgt ttatgcaatg gatggagcaa ttacccgcgc gggagttaac
      841 gcgcgggcaa taaaactaca ggcggtggcg ataatcgctg ggagtgcgat caaactgccg
      901 acggaaaacg cgggagaagg tctgcggcga gacataaccc aggtccattg cgatatcaaa
      961 aatcggacgc tcggtggtgc gcaactcaac ggcggccagt aacaggcgac gttggcgaat
     1021 gtaatcgcca agcgtctgat gcgtcaccgt gcggaacatt cgttgcaagt accactttga
     1081 atagcctgat ttttttgcga ctacatcaat gttaagcggc tggtcaatat gctcgtcaat
     1141 ccatgcgata agatcctgaa taattttctg atgggacata aatctgcctc ttttcagtgt
     1201 tcagttcgtt aattcatctg ttggggagta taattcctca agttaacttg aggtaaagcg
     1261 atttatggaa aagaaattac cccgcattaa agcgctgcta acccccggcg aagtggcgaa
     1321 acgcagcggt gtggcggtat cggcgctgca tttctatgaa agtaaagggt tgattaccag
     1381 tatccgtaac agcggcaatc agcggcgata tagacgtgat gtgttgcgat atgttgcaat
     1441 tatcaaaatt gctcagcgta ttggcattcc gctggcgacc attggtgaag cgtttggcgt
     1501 gttgccagaa gggcatacgt taagtgcgaa agagtggaaa cagctttcgt cccaatggcg
     1561 agaagagttg gatcggcgca ttcatacctt agtggcgctg cgtgacgaac tggacggatg
     1621 tattggttgt ggctgccttt cgcgcagtga ttgcccgttg cgtaacccgg gcgaccgctt
     1681 aggagaagaa ggtaccggcg cacgcttgct ggaagatgaa caaaactaaa gcgccacaag
     1741 ggcgctttag tttgttttcc ggtctttgtc tttctctcta tcccgctggt acacaggagg
     1801 gtttccccga cgtcaacaca cctcattcga gcacgtggtg gaggttccgg ttggtgttga
     1861 tgctttaatt gtatgtcacc gacgtttctt cgccagtgta aaagtatact ttttaaccgc
     1921 aatatttttg tcatctcaga cgatttttta tcgcaatcct gaacggtata cggctcgata
     1981 acgctgcaat cttgcgcacc gacgataacg tttgcgcatc aattgcctgt tttttcatcg
     2041 tcaagacaat aaaagagaaa aaagcagcaa acttcggttg aaaaagccgc tatgatcgcc
     2101 ggataatcgt ttgctttttt taccacccgt tttgtatgcg cggagctaaa cgtttgcttt
     2161 tttgcgacgc agtaaattgt cgcaaacctg gagcaggaag ataacgtttc gctggcaggg
     2221 gattgtccgc cacgcatctt gacgaaaatt aaactctcag gggatgtttt cttatgtcta
     2281 cgccatcagc gcgtaccggc ggttcactcg acgcctggtt taaaatttca caacgtggaa
     2341 gcactgtccg tcaggaagtg gttgccgggt taacaacgtt tctggcgatg gtctactcgg
     2401 tcatcgtcgt tccaggtatg ttgggtaaag caggcttccc gcctgcggca gttttcgttg
     2461 caacctgtct ggttgccgga ctcggttcta tcgtgatggg tctgtgggct aatctgccgt
     2521 tggcgattgg ttgcgccatc tccctgacgg cgtttaccgc attcagcctg gtgctggggc
     2581 aacatattag cgtacctgtc gcgctgggtg ccgtgttcct gatgggtgtg ctgtttacgg
     2641 taatttctgc cacgggtatc cgtagctgga ttttgcgcaa cttgccgcac ggtgtggcgc
     2701 acggcacggg gatcggtatc ggtctgttcc tgctgctcat tgccgctaat ggtgttggtc
     2761 tggtgattaa aaacccgctt gatggtctgc ccgttgcgct gggtgatttc gcgaccttcc
     2821 cggtgattat gtcactggta ggtctggcgg tgatcatcgg cctggaaaaa ctgaaagtcc
     2881 ctggtggcat tctgctgacc attatcggta tctcaattgt cggtttgatc ttcgatccta
     2941 acgtccattt ctccggcgtt ttcggcatgc cttcattgag cgatgaaaac ggcaattcac
     3001 tgattggcag cctggacatt atgggcgcgc tgaatcctgt agtcctgcca agcgttctgg
     3061 cgctggtgat gacggcagta tttgatgcca ccggaactat ccgtgccgtc gccggccagg
     3121 cgaacctgct ggataaagat gggcagatca tcgacggtgg gaaagcactg accactgact
     3181 ccatgagcag cgttttctct ggcctggtgg gtgctgctcc ggcagcggta tacatcgagt
     3241 ctgcggcggg tacggcggcg ggcggtaaaa ccggtttgac ggctatcacc gttggcgtgc
     3301 tgttcctgct gattctgttc ctctctccgc tctcttacct cgttccgggg tatgcaacgg
     3361 ctccggcgct gatgtacgtt ggcctgctga tgctgagcaa cgtggcgaaa atcgactttg
     3421 ctgattttgt tgatgcgatg gcgggtctgg ttacggcggt attcatcgtg ctgacctgta
     3481 acatcgtaac aggcatcatg atcggcttcg cgactctggt gattggtcgt ctggtttccg
     3541 gcgaatggcg caagttgaac atcggtacgg tcgttatcgc cgtggcgctg gtgaccttct
     3601 atgcgggtgg ctgggctatc taatctttca tcggattctg aaaacgggtg gcaatggctg
     3661 cccgttttta ttttctccgc acattgtgtg agcttttgcg atattctgaa aaaatgagaa
     3721 ttcaggcata acgcctttct cagatcataa aaaaacatcg caaacaggga acgcatggaa
     3781 atcttcttca ccatactgat aatgaccctc gtggtctcgc tgtccggggt ggtcactcgt
     3841 gtcatgccct ttcagatccc gcttccgctt atgcaaatcg ccatcggtgc gctactggcg
     3901 tggccgacgt ttggtttgca tgtggagttt gatcctgaac tctttttagt cttgtttatc
     3961 ccgccgttgc tgttcgctga tggctggaaa acgccgaccc gtgaatttct cgaacatggt
     4021 cgagagattt tcggcctcgc gctggcgctg gtggtggtca ccgtggtcgg cattggcttc
     4081 cttatttact gggtggtgcc gggcattccg ctgatccccg cctttgcgct ggcggcggta
     4141 ctttctccga ccgatgctgt ggcgctctcc ggaattgttg gcgaagggcg catcccgaaa
     4201 aaaatcatgg gcattttgca gggcgaagcg ttgatgaatg acgcctccgg cctggtgtcg
     4261 ttgaagtttg ccgtggcagt ggcgatgggg acgatgatct tcaccgtcgg cggtgcgacg
     4321 gtcgaattta tgaaagtagc cattggtggt attcttgccg gttttgtggt gagctggctg
     4381 tatggtcgct cgctgcgatt cctcagccgc tggggcggtg atgaacccgc gacgcagatc
     4441 gttctgctgt tcttgctgcc attcgcttct tatctgattg ccgaacatat tggcgtttcc
     4501 ggcatcctcg ctgcggttgc cgccgggatg accatcaccc gctccggtgt gatgcgccgt
     4561 gcgccgctgg caatgcgcct gcgcgcaaac agcacctggg cgatgctgga atttgtcttt
     4621 aacggcatgg tgttcctgct gttaggtctg cagctgccgg gtattctgga gacgtcgctg
     4681 atggcggcag aaatcgaccc taacgtcgaa atctggatgc tgtttaccga tattattctg
     4741 atatatgcgg cgctgatgct ggtccgtttc ggctggctgt ggacgatgaa aaagttcagc
     4801 aaccgcttcc tgaagaagaa gccgatggag tttggttcgt ggaccacacg agaaatcctg
     4861 atcgcgtctt tcgccggggt gcgtggggcg atcactctgg ccggtgtgct ctctatcccg
     4921 ctgctcttgc cggatggtaa cgtcttcccg gcgcgctatg agctggtgtt cctggcggct
     4981 ggcgtcattc tcttctcgct gtttgtcggt gtggtgatgt tgcctattct gctacaacac
     5041 attgaagtcg cggatcattc gcaacaattg aaagaggaac gtattgcgcg agcggcaacg
     5101 gcagaagtgg cgattgtggc gatccagaaa atggaggagc gtctggcggc ggataccgaa
     5161 gagaatatcg ataaccagct gcttacggag gtcagttctc gcgtcattgg taacctgcgt
     5221 cgtcgcgccg atggacgtaa cgacgttgaa agttccgtgc aggaagagaa ccttgagcgt
     5281 cgcttccgtc tggcggcatt gcgttctgaa cgtgctgaac tgtaccacct gcgcgccacg
     5341 cgggagatca gcaacgaaac gctgcaaaaa ttactgcacg atctcgattt gcttgaagcg
     5401 ttgctaattg aggaaaatca gtaagccgaa agcgacgtaa ccaggcccga ggtaatctga
     5461 aaaaggtgca atcttttgca cctttaacag gttagctcgg gctaacattc atgattctaa
     5521 aacaaataag ttatctctct tgtaaagtca attcattggc tgttcttcga cttaacctca
     5581 atctgcgtgt tgcataatat ctgcagtaat cttcatagcg actgcattaa tattatcctc
     5641 agtttgtgac atgccagtat gagaggtaca atcttcttca aagaatttct gtagtgttac
     5701 cagggtatca ctatcaatgg ggatattttc ctctccacgg agtatggaaa tgcccattcc
     5761 ctggacgcta gcctcatcaa aattaacccg atagagacgg gtattaaaca t
//
LOCUS       DADLFA010000211         5691 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000211 DADLFA010000000
VERSION     DADLFA010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5691
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.213"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(260..1018)
                     /locus_tag="JQH65_003641"
     CDS             complement(260..1018)
                     /locus_tag="JQH65_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAY6140917.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFTHPKSERTRAFLSQVIH"
     gene            complement(1026..2129)
                     /locus_tag="JQH65_003642"
     CDS             complement(1026..2129)
                     /locus_tag="JQH65_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAY6140918.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     VLLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2139..3309)
                     /locus_tag="JQH65_003643"
                     /pseudo
     CDS             complement(2139..3309)
                     /locus_tag="JQH65_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3377..4456)
                     /locus_tag="JQH65_003644"
     CDS             complement(3377..4456)
                     /locus_tag="JQH65_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY6140919.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LLGFSYADADGKFSGIDVDVDVDVDVDVDVDVDVDVDVCRSVAAAVFGDDTKVKYTPL
                     TAKERFTALQSGEVDLLSRNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAK
                     ELDGATVCIQAGTDTELNVADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQS
                     QLYALRIKLSNPAEWIVLPEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGIN
                     SQNVDEKAANPATPDMAHLLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSE
                     SPLKIKRGQNNLWNNGGIQYAPPVR"
     gene            complement(4887..5108)
                     /locus_tag="JQH65_003645"
     CDS             complement(4887..5108)
                     /locus_tag="JQH65_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6140920.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGCLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            complement(5361..>5691)
                     /locus_tag="JQH65_003646"
     CDS             complement(5361..>5691)
                     /locus_tag="JQH65_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HAY6140921.1"
                     /translation="FMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRM
                     RLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFLPVFFVV
                     IRRCFKG"
BASE COUNT         1614 a         1452 c         1315 g         1310 t
ORIGIN      
        1 cccatgcgag agtagggaac tgccagacat caaataaaac aaaaggccca gtcttccgac
       61 tgagcctttt gttttatctg ttgtttgtcg gtgaacactc tctcgagtag gacaaatccg
      121 ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg
      181 ccataaactg ccagacatca aatcaagcaa aaaggccatc cgaaaggatg gcctttttgc
      241 ttttcgaact aacattcaat taatggatta cctgcgataa aaatgccctc gtacgctctg
      301 atttaggatg cgtaaaaaat tcatcaggtg cagcttgttc cactatctcc ccacgatcca
      361 taaaaattac ccggtcagcg acggtacgtg caaaccccat ctcatgtgtt acacacaaca
      421 ttgtcatacc cgactgcgcc agcccaatca tcgtatccag cacctctttc accatctccg
      481 gatcgagcgc cgacgttggt tcatcaaaca acataatttt cggcttcata cacagcgaac
      541 gcgcaatggc aacgcgttgc tgctgaccac ctgaaatctg tccgggaaac ttatgcgcat
      601 gttcggcaat tctcacccgc tcaaggtaat gcatcgccag agcttcagcc tctttcttag
      661 gcatcttgcg tacccaaatc ggtgccaggg tacagttctg taaaacggtc agatgaggga
      721 agagattgaa atgctgaaat accattccca cttcttgcct gacgcgctca atattgcgga
      781 tatcttcatt aagttcgatg ccatctacga caatccgtcc ctgttgatgt tcttccagat
      841 gattaataca acgaatggtt gtcgatttac cggaaccaga agggccacac agaacgatcc
      901 gttctcccgg ttgcacggtt aaatttatat ttttcaaaac atggaattgt ccataccatt
      961 tatggacgtt ttccagcgta atcatcgcgt tagcaggttg cagtaaaatt tggctcatag
     1021 tgtcctcaat gcggtgtacg cccggtgtta aaacgttttt ccagatgttg gctataacgt
     1081 gacatgctga aacaaaagat ccagtagatc agcgcggcga aaacataccc ttccgtcgac
     1141 atacccaacc aggcgggatc aacggttgcc tgctgaacgc tactgaaaag atcgaacaag
     1201 ccgatgatga tcaccaggct ggtatctttg aagagtgcga tgatggtatt taccagccca
     1261 ggaattacca gcttcaacgc ctgtggcaga ataaccagcc cctgagtttt ccagtacccc
     1321 aacgccagcg actctgccgc ttcatattgc cctttaggta gggcctgtaa tccgcctcgc
     1381 acgacttccg caacatatgc tgactgaaac aggatcacgc caaccagcgc gcggatcagt
     1441 ttgtcgatac tggtgccttc tgccataaac aacggcagca tgaccgaaga cataaacaat
     1501 acggtaatca gcggtacgcc gcgccagaac tcgataaaaa tgaccgataa gatacgcaca
     1561 atcggcatat gggagcggcg acctaacgcc agtaagatcc cccacggtag cgcccccgca
     1621 atcccaacgg atgcaataat taaagttagc gtcagcccgc cccattgccg ggtttcaacc
     1681 cgctcaagac caaaaaaacc gccatacatc agccaccaga caatcagtgg gtaaatcacc
     1741 gcccagaccg caatatagcg accgcgatgc gggagtattt tccagaacat tggtgcgatt
     1801 gacacaaggc caatcagtaa taccaggtta attcgccagc gttggtcgtg tgggtaaagt
     1861 ccatacataa actgaccaaa tcgttcgtgg atgaagaccc aacaggcacc ggcttttgtg
     1921 cagtcggcac gcgttgagcc aacccagtta gcctgcaaaa atgcccagtt cagcaacggt
     1981 gggatcagtt cccacatcaa ccatatgcag ccaatagtca gcaggctatt gctccagctg
     2041 gagaacagat tttttcgcac ccacaccatc gcgcggcttg agttatggct cgctgggcgc
     2101 gagggctgag acagcaatac ttttgtcatc atgactcctt agcgttcaac gatcgcgatg
     2161 cggcggttat agatattcat cagcagcgag atagtcaggc tgataatcag atagaccgac
     2221 atggtcatgg ctatcgtctc gatggcttgc cccgtctggt tcagcacggt gccagcaaac
     2281 agcgaaacca tatcgggata gccaatagcg gcggcaagag aggagttttt gacgatgttg
     2341 agatactggc tggttaacgg cggaataatc actcgcagtg cctggggaat aatgacctgg
     2401 cgtagcgtaa ccccagtgac cgagccgctt catgttgacc ataaggcact gcctggatcc
     2461 cagcgcggat aatctcggcg ataaatgcag aggtataaac cgaaagtgcc agcgttaagg
     2521 ctgccagttc aggaattaaa accattccgc cgcggaagtt aaagcctcgt agggctggga
     2581 catcccagtg tagtgctgcg ccaaaaagcc attgcgccag taaaggcaaa ccgatgatca
     2641 acactgcggc gaacggccag gtgcggcgca gttgtccggt ctttatctgg tatgttttat
     2701 taaagcggaa tagcccaaca gaaaggacta tagccataac aacagccagg ataaacgcaa
     2761 taaatccgtc tcccagctgc ggtgacggaa tataaagccc acgattgctc aaaaaggcca
     2821 gatcaaacgc gctcacagcc tggcgcgtgg tccgggcaaa ttgcgcaaca cggcaaagta
     2881 ccagaagaag atttgcagca gcgggggaat attacggaag atctcaatat aaattgtgga
     2941 tagctttcgt agcagtcagt tatccgaaag tctcgtcaga ccgataaaga agcctaaaac
     3001 agaagcgaac acaatacaca atgcagaaac cagtagcgta ttgagtaagc cgacaataaa
     3061 aacgcgtccg taggtgtcgc cctgctggta atcgatcaaa tgctggacaa taccgaagcc
     3121 agcgccgcga tccagaaagg caaaacctga agtaatgcca cgattactga gattcgttac
     3181 agtgttgtga aataaccaac caacaattcc gacaacagca acaacggcaa ggatctggaa
     3241 taaccaggca cgaaccgtag ggttggcaaa ggagagtgag cctttaacgg ttgagcggcg
     3301 atgagacata gaaacctcgg aaagcataac gctgaagaga gcgccgccgt agcgacgctc
     3361 gttacctcac accgacttaa cgcacgggcg gcgcgtactg aataccgccg ttattccaga
     3421 gattattttg cccacgttta attttcagcg ggctttctga acctacgtta cgctcgaaaa
     3481 tttccgagta gttacccacc tgtttgatga tgttataggc ccatttatta tccaacttca
     3541 gatccttgcc gtaatcgcct tctttaccca gcagatgtgc catatcaggc gttgctggat
     3601 tagccgcttt ttcatcgacg ttctgggaat tgatgcccat ctcttcagca ttcagcatgg
     3661 cgaaaagcgt ccagcgtaca atcgagaacc attcatcatc gccacgacga actaccggcc
     3721 caagcggttc tttagagata acctccggta agacaatcca ttcagctggg ttgcttaatt
     3781 tgatgcgcag ggcatacagt tgcgactgat ccgaggccag cgtatcgcag cgaccagatt
     3841 ccagtgcctt cgctgattcg tcagagcgat cgaaagtcac cggtgtgtac ttcatattgt
     3901 ttgccttgaa atagtcggca acgttgagtt cggtatcagt acccgcctga atacagacgg
     3961 tagcgccatc cagttctttc gcgcttttta gccccgcttt atcgtgcgtc aggaagccaa
     4021 tgccgtcgta ataagtgacg ccagtaaatg ccattcccat cccggcgtcg cgagatgaag
     4081 tccaggtcgt attacgggag agcaaatcca cttcccctga ctgtaaagcc gtgaagcgtt
     4141 cttttgctgt gagtggggta tatttcactt tcgtgtcgtc accaaataca gcagcggcaa
     4201 cactacgaca aacatcaaca tcaacatcaa catcaacatc aacatcaaca tcaacatcaa
     4261 catcaacatc aacatcaata cctgaaaact taccgtctgc atcggcataa gagaacccaa
     4321 gtaatccatc actgatcccg cattgcacaa aacctttttt ctgaacggca tcaagcgtcg
     4381 cgccagcatg cgcctgattt gcaacggcaa gcagcacgct ggcggcagcc agtgtggcta
     4441 tcatcatctt tttcataatg cttcctgtga ggcgaaaatt atcgttatag gacgtttggg
     4501 aaacgtcttt tacctgtctg tggccatcct cttataagca aaagtagtgc cagcttttgc
     4561 gcacgatttc gcgtcgcaat aagctgagtg tagacaggaa ataataaaaa cagcgccccg
     4621 caatggtgcg aaaatgaaac atgcgccaca ttttggcgca aaatatttac gcgtgtttta
     4681 ctaagcagaa taatgattgt tagcgtgagt atatttctgg tgggaagtgt gaaagttaaa
     4741 ttgatgcgtt atttcgaaag cagcgaaata acgcgatata acatgctaaa tcagcagaga
     4801 attagccagc agaatatagc aatattttat cttatgtaaa gatactgtat cgttaaacag
     4861 agcgcgaaac tccgcgccct gttcagttat ttcgacagag cgatgatccc catcgtcagg
     4921 ccagcaacag ccgctgcccc gccggcaata gccccagccg tttcccagtt atcctgagtc
     4981 gtgcctttca tacaggtatt ggtgtgaacc agacaaccct gatcgtcata aaccggaacg
     5041 cagggcgaat cgtgagcaca gcccgccagc aatgcggtga gcaatgccac aggaattaat
     5101 cttttcatgg tgttacctca gtaataaacg ctaacgctta ataagttaac tctcttatta
     5161 acgagctgaa tgccatttta tcaccggagg ttataaatat tgcggaatga ataatgacaa
     5221 attatgtgaa ttgtaaaaat agtgaaggga gcgggaacta tagaaagttt ttaaagagtg
     5281 agtggaagaa tatcttatta atttttatgg gtttcactga aaataataaa ggcacccgaa
     5341 agtgccttta tgtttctgat ttatccttta aagcaacggc ggatcaccac aaagaacacc
     5401 ggtaggaaga agattgccag caacgttgca gagaccattc cgcccattac cccgataccc
     5461 actgcgttct gcgcgccact gcctgcaccg ttactgatag ctagcggtaa tacgccgaga
     5521 ataaaggcga gagaggtcat caggatagga cgcagacgca tacgtactgc cattagtgtc
     5581 gcttcaacaa cacctttacc ctctttctcc atgagatctt tagcgaactc aacaatcaaa
     5641 atagcgtttt tggccgacaa gccaattgtc gttagcaagc ccaccataaa g
//
LOCUS       DADLFA010000212         5604 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000212 DADLFA010000000
VERSION     DADLFA010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5604
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.214"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(1..217)
                     /locus_tag="JQH65_003647"
                     /pseudo
     CDS             complement(1..217)
                     /locus_tag="JQH65_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(361..3669)
                     /gene="icsA"
                     /locus_tag="JQH65_003648"
                     /gene_synonym="virG"
     CDS             complement(361..3669)
                     /gene="icsA"
                     /locus_tag="JQH65_003648"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HAY6140922.1"
                     /translation="MNQIHKFFCNMTQCSQRGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFAIPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSIINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEITGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYGWFNNTVKGDGFTGEKYSSKGITGALEAGYIYPTIRWTTHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGVNNIQAKLGLRTSLISHSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4198..5400
                     /gene="virA"
                     /locus_tag="JQH65_003649"
     CDS             4198..5400
                     /gene="virA"
                     /locus_tag="JQH65_003649"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HAY6140923.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDVLLKIITFG
                     IYSPHETLAEKYSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFPVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQLNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
BASE COUNT         1750 a         1261 c          927 g         1666 t
ORIGIN      
        1 tttcaacatc caatattcct cgtacagaac tactcaagtc ctgaatagaa aaagttccag
       61 ctggtacagt agagtcatat attgctcgac cttgttgaga tacaacaaca cgtgcattag
      121 tttctgcaat accagtaatc tgaggcgcat aaccacgaat atttggcggt agcattcgtt
      181 catcgctttc taatgcaatt cctgtgtaac gccaagcaac agcgcattct ggcgcatgat
      241 ctggtaaaca cgtttggcat tgatcgcagg cataccatca tgtgcacaaa aacccaccga
      301 gcttttacac ggtgggtccc agagaaatgc aggacatcaa cacgccctgc atttttatta
      361 tcagaaggta tatttcacac ccaaaatacc ttgggtgtct ctgtaactgt tattacccaa
      421 ttgctgagat acgtttcccc agatgcttag gttatctgca agacgccccc ccacaccagt
      481 tttgagttca atcacattac ggttgcctat ctggtgattt gacatgccat tcataattac
      541 accatattgc tttgagctcc atttccaatt cacttccaaa aatggttcga acttacgaag
      601 agtctcctta tcgatacaac tatgactaat taaggatgta cgcaatccca attttgcttg
      661 aatattattg accccagagg atgtgaccat agtgccattg tgttcaatat agtcgtttgc
      721 ttttacccca tgcctagtta tctggacttg tggattgaga tacaatgcgt tgtcaatatt
      781 attatgagta gtccagcgta tggttgggta gatatagcca gcttccaaag ctcctgttat
      841 tcctttgctg gaatatttct caccagtaaa cccatctcct ttgactgtat tattaaacca
      901 accgtactgc aaccaagcat ccatatatag acctgttcgt tctttttcat cctgaaacca
      961 agaactgtac aaaccggcag tataaccgct cacagttcct cgtgaactat aactagtata
     1021 cttattatga gttttgctat cctgatatcc ataagctccc ataataccca catgcaagcc
     1081 atcatgatca gtgaagtttg tcttcaaaat atctccacca atctgataaa ccatcctgtt
     1141 agctgtggtt tttaattgtc tatcagctaa attatgctca ttatgtccac caacagtacg
     1201 aatccacatg gtggtttcgc tgagctgacc tgtagtagga tcaacaatag tgctaccgtg
     1261 tcggtcatag agagaaggac taaacatcgt gttcattgct atcaggttag caatataact
     1321 gccagcttca gggcgccatg tgtgaatact accttcaccc aaattaagtg aagatacaag
     1381 ctgtgaacta gctcggcgac tactcatttg agtagactct tgattgctca tctgttttga
     1441 ttcttgatta tccatctgac tagttagata ccacttattg gtattcagtc cagatacagt
     1501 gccctgtttc aggcgatagt cataactacc agcaacaata cggcctttct gaataaaagc
     1561 attcttatca gaagaatctg tcgaaataat ttgaacacct tcaagagttt tatctccaga
     1621 gcctccctca tttgtcactg caacataggt aataccagac gcatggcctg taatttccag
     1681 ccggtcagtt ttactgttgt cattgccgag aaccgcgccc aaagataata ttccgccctt
     1741 tccatgccaa ttacctttct gaacataggt ctgaccgaca tttgacgaac tattattgag
     1801 aataacatga ccgtttttct ccagagtcat gttacctgtg acaatgacgg ggtcaggcaa
     1861 aaaaggggca ttaatattat tgattagcac agtcccacta tttaataaag tgttaacagt
     1921 ttggttcatg cctgaaaaat tcaaggtagc acctttatta acaataacac cagcggtacg
     1981 tgtcatagct tcaactgtcc ccatttttag aatgcctgca ttgatgttgg tgaaaccaga
     2041 gtaggtattt ttttccgcca aaatgagagt tccctcccct gattttgtta gggattttcc
     2101 gtcccagcta tttatattat gatggctgag attatctgcc agaatagtac taacagtaag
     2161 ttcagcgttt tctttcagat tgaactctcc atgactgtcg ccatctgtat cattccagct
     2221 caaaccgtag atcaatttat tctggtcact gatatagctt tttgtatagt caatagcacc
     2281 agtagtgagt aacggattga tgatagatat attagaaaca ctgaaacctg ttatcccttt
     2341 atctgcactt agcagtttat agtccttctg cgcccagtta tttataatag cttccacttt
     2401 tacattgttg ccaaatgaca tatttccaga agcttccaga ctactgattc cagctaaatt
     2461 taaagaactg ttctcaccaa aagaaacgtc acctgaaaca gtgaatttgt tacctgatag
     2521 tgaaacagtt gtacaatcac cagcacataa atcaccttca atagttggtg tggtcccagt
     2581 gtaatcattg ttgataattt tcagaatgga tgaattgtta acctgtacat caccagtaat
     2641 gacagaacct tcatggagta ttaatgaatt gtttccacca gtgatgtgga tagcattacc
     2701 tgctacattt tgtgttccat catcttcttt acctgaaatt atatatccgc tattagcaat
     2761 ggtcatattg ctaccattta tagcatcccc ccaatgtcca ccattaccgc ctaaaatagc
     2821 tccattgttg attatagaca ggttatctcc tgtgatagca ttaccaccgt agccatcata
     2881 accataaccg taagcagcac ctccgttgcc tccagcgaac gtgcccttat tgattacaga
     2941 gaggctgctt cctgtgatgg catcacctcc attaccgcca tcaccctcac cataattgtt
     3001 acctccatta ccaccggaaa tagtcccttt gttgattatg ggtagattga ctcctgtgac
     3061 ggcatcacct ccattccccc catcactatc actataacta tcgccaccat gtccacctga
     3121 aataatttct ccatttatta taaacagatt gtctcctgta acagcctcac caccatcacc
     3181 gttatggtca gcaccgctac cgccgctacc accaagaatc atgccttgat tgattataga
     3241 caagtcagat ccggtaatag agtcaccacc attaccgcca caactattac catcattatt
     3301 atcaccagca ccaccatgac ctgcgctaat cataatatca gagttaatcc gtagcttagt
     3361 tctagatgca tgagaggggt tggaagaagt tatataccac gcatccattc catcttcacc
     3421 agctcccaag ggagaaagtc catcaacagg tgttaataat ttttcataat tgtcctctga
     3481 aaaatgaagt tcttgagtac ccgaaagagg aatagcaaaa gctattggcc ccccgagcaa
     3541 cagggatgca ccaacaacaa aaggagaaaa agacaatttg catgtttttt cctttaccgt
     3601 aggtaattct ccggcccccc tctgtgaaca ttgggtcata ttacaaaaaa atttgtgaat
     3661 ttgattcatg cactatatta tcagtaagtg gttgataaac ccctaaaaag attccgtttt
     3721 atccggaata aagggacgat atatgcaaaa acatattaaa caaagcctca accaaaacat
     3781 gagagctgtg ttatatatca atgcaacggg agaaatttac ctgtgtcttg ctttgaaaaa
     3841 aagaggggga aagctatatg ttcgataata aattaataat tttactatga tagtctgctt
     3901 cagaccgcaa cttttcagtt ctttctgtac gcttgcccaa agtttaacca ctttgttttt
     3961 ctatacaccc aatggttcta tctgcccctt gatgttaatc cccaaagcca ataacatgaa
     4021 tgtattcata tcacaacttt cctaccagac tctccaatgc aataactgca taattgtttt
     4081 tagtggaaat gtatcgtttt cttaaagaga agaataacat tccattttat tatgttccca
     4141 atttaattat attaatatat attggcatca ataggaaaat acatcaggag aaatcaaatg
     4201 cagacatcaa acataactaa ccatgaaaga aatgactcat catggatgtc aactgtcaaa
     4261 tcaactacag aagtaagttg gaataaattg agtttttgtg acgttttact gaaaataata
     4321 actttcggaa tatattctcc acacgaaact ctagccgaaa aatatagcga aaaaaaacta
     4381 atggatagtt tctcaccatc actatcacaa gataaaatgg acggagagtt tgcccacgca
     4441 aacatagatg gcatttcaat acggctatgc cttaacaaag gcatatgttc ggttttttat
     4501 cttgatggag ataaaataca atccactcaa ctttcatcaa aagaatataa taacttgtta
     4561 agttcactcc cccccaaaca attcaactta ggaaaagtac atactattac agctccggta
     4621 tcagggaatt ttaaaactca caagcctgca ccagaagtta ttgaaacagc cattaattgc
     4681 tgtacatcga taataccaaa cgatgattac ttccccgtta aagacactga ttttaatagt
     4741 gtttggcatg atatttacag agatattcga gcatctgaca gcaatagtac aaaaatatat
     4801 ttcaacaata ttgaaatacc actaaaatta attgcagatc taattaatga gttaggaatt
     4861 aatgaattca ttgatagcaa aaaagagctt caaatgctaa gttacaacca attaaataaa
     4921 ataatcaatt caaacttccc ccaacaagac ctatgttttc aaacagaaaa acttttgttt
     4981 acttcactct ttcaagaccc tgcatttata tccgcactaa catctgcatt ctggcaatct
     5041 cttcacatca cgtcttcctc tgtggaacac atatatgccc aaattatgtc cgaaaacata
     5101 gaaaacaggc ttaatttcat gcctgaacaa cgagttatta acaattgtgg acatattata
     5161 aaaatcaacg cagttgtccc taaaaacgac actgcaatct ctgcctctgg ggggagggct
     5221 tacgaagtta gctcatcaat attaccatcg cacataacat gtaatggtgt tggtattaat
     5281 aaaatagaaa caagctatct cgttcatgct ggaacacttc caagcagtga agggcttcgc
     5341 aatgctatcc ctccagaaag tcgtcaagta tcatttgcca tcatatctcc tgatgtttaa
     5401 gccaatatta tattggctgc cagactgtaa aaaaacgttc ttcagaacag ctctataaac
     5461 tattaaacca aagctaaaag gatggggacc aatagaatct cagcccatcc tttttatatc
     5521 attatgaacc gccccggaaa tcctggagac taaactccct gagaaagagg taaacaggat
     5581 gactaaaaat acacgttttt cccc
//
LOCUS       DADLFA010000213         5602 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000213 DADLFA010000000
VERSION     DADLFA010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5602
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.215"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(35..772)
                     /gene="bamD"
                     /locus_tag="JQH65_003650"
     CDS             complement(35..772)
                     /gene="bamD"
                     /locus_tag="JQH65_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HAY6140924.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENSYRQMQMNAQAEKVAKIIAANSSNT"
     gene            907..1887
                     /gene="rluD"
                     /locus_tag="JQH65_003651"
     CDS             907..1887
                     /gene="rluD"
                     /locus_tag="JQH65_003651"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HAY6140925.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNLDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPEDMVELIEVMRADFEEHKDEVDWL"
     gene            1884..2615
                     /gene="pgeF"
                     /locus_tag="JQH65_003652"
     CDS             1884..2615
                     /gene="pgeF"
                     /locus_tag="JQH65_003652"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HAY6140926.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     EHVEENRKQLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2745..5318
                     /gene="clpB"
                     /locus_tag="JQH65_003653"
     CDS             2745..5318
                     /gene="clpB"
                     /locus_tag="JQH65_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAY6140927.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1397 a         1336 c         1519 g         1350 t
ORIGIN      
        1 ctcaagggct gccgttttgt gtttcaggtt tctgttatgt attgctgctg tttgcggcga
       61 tgattttcgc tactttttca gcttgcgcat tcatctgcat ctgacggtat gaattttcca
      121 tcagtggcag cgcatcacgc gtagcctggg tatccgggta gtcgcgcaac atgccttcta
      181 cgcggttaac gacggcaacc catgcaccac gttctgtata gtactcggca acggagtatt
      241 catatttcgc cagacgatct ttcaggaata ccagacgttt ggttgcatcg gtagtgtact
      301 gactgttcgg atagccgcgc accagtttgg aaaagtcact aaacgcagct cgtgcatgtt
      361 gaggatcgcg atcgctacgg tcaacgccaa agaacccttg cagcgcactg tcatccagcg
      421 ccatattggt caggccacgc atgtacatga cataatcgat attcggatgg gtcgggttaa
      481 ggcgaataaa acgatcgatg gcagcctgtg ctaacggcaa atcggcgttt ttatagtagg
      541 cgtagatgag atccagctgc acctgctgcg aatacggacc aaacggatag cgattatcta
      601 atgcttccag ttgcgttatt gcctgtctcc agttaccgtc ctgcagcttt tgttgtgcag
      661 tcgcgtaaat ttcatttggt ggattatcag gtacttcttc ctttgacccc gagcaacccg
      721 ccaaaaacag gcttagtgtg gcggctgcca ccagatattt catgcgcgtc atgacgtttt
      781 gactttcctc aaaatgtaat acgggagatt ctctgttcct gctcccggtt aagaccagct
      841 acaatagcac actatattaa acggcaaagc cgtaaaaccc caacgataaa cgaagaagca
      901 gtatatatgg cacaacgagt acagctcact gcaacggtgt ccgaaaacca actcggtcaa
      961 cgcttagatc aggctttggc cgaaatgttc ccggattatt cacgttcgcg aataaaagaa
     1021 tggatcctcg accagcgcgt gctggtaaac ggcaaagttt gtgataagcc gaaagaaaaa
     1081 gtattgggtg gcgagcaggt tgccatcaac gctgagattg aagaagaagc gcgttttgaa
     1141 ccgcaggata tcccgctgga tatcgtctat gaagatgaag atatcatcgt cattaataaa
     1201 ccgcgcgacc tggtggtaca tcctggcgcg ggtaacctgg atggcacggt actgaatgcg
     1261 ttgcttcatt attatccgcc cattgccgat gtaccgcgtg cgggcatcgt ccatcgtctg
     1321 gataaagaca ccactggcct gatggttgtg gcaaaaaccg ttccggctca gacgcgttta
     1381 gtcgaatctt tgcaaaggcg tgaaattact cgtgagtatg aagcggtggc aattggtcat
     1441 atgaccgctg gcggtacagt agatgaacca atcagccgcc acccgaccaa acgtactcac
     1501 atggcggtgc atcccatggg caaaccggcg gtcactcact atcgcatcat ggaacacttc
     1561 cgtgtgcaca cgcgtctgcg gttgcgtctg gaaaccggac gtacgcacca gatccgcgtg
     1621 catatggcgc atattaccca tccgctggtg ggcgatccgg tttatggtgg ccgtccgcgt
     1681 ccgccaaaag gtgcttcgga agcgtttatc tccacgctgc gtaagtttga ccgccaggcg
     1741 cttcatgcaa ctatgctgcg tctttatcac ccgatctccg gcatcgaaat ggaatggcat
     1801 gcgcctattc cagaagatat ggtggagttg attgaggtta tgcgcgccga tttcgaagag
     1861 cataaggatg aagtggactg gttatgagta agctgattgt cccgcagtgg ccgctgccaa
     1921 aaggtgttgc ggcctgtagc tctacccgta tcggcggcgt gagcttgcca ccttacgact
     1981 ctctcaacct tggcgctcat tgtggtgata acccggagca cgtagaggag aatcgcaagc
     2041 aactttttgc tgcgggaaat ttgccttcaa aaccggtctg gcttgagcag gtacacggca
     2101 aagatgtgct taagctcact ggcgaacctt atgcctcaaa acgggcggat gcctcttata
     2161 gcaatacgcc cggcacggtt tgcgcagtga tgactgccga ctgcctccct gtgctgtttt
     2221 gcaatcgagc gggaacggaa gtcgccgccg ttcatgctgg ctggcgtgga ctgtgcgcag
     2281 gcgtgctgga agagacggtt tcctgttttg ctgataaacc tgaaaacatt ctcgcctggt
     2341 tagggccggc aattggtcca cgcgcgttcg aagtgggggc ggaggttcgc gaggcgttta
     2401 tggcagtaga cgctaaagca agtgcagctt tcattcagca tggtgataag tatctggcgg
     2461 atatttatca gcttgcccgg cagcgtctgg cgaacgtggg tgttgagcaa attttcggcg
     2521 gcgaccgttg tacatatacg gaaaatgaga ctttcttctc ttatcgtcgc gacaagacca
     2581 ccggtcgtat ggcaagtttc atttggctga tataacctaa agaatcaaga cgattcggta
     2641 cgcgtgattt tcttttcaca ttaatctggt caataacctt gaataattga gggatgacct
     2701 catttaatct ccagtagcaa ctttgatccg ttatgggagg agttatgcgt ctggatcgtc
     2761 ttactaataa attccagctt gctcttgccg atgcccaatc acttgcactc gggcacgaca
     2821 accaattcat cgaaccactt catttaatga gcgccctgct gaatcaggaa gggggttcgg
     2881 ttagtccttt attaacatcc gctggcataa atgctggcca gttgcgcaca gatatcaatc
     2941 aggcattaaa tcgtttaccg caggttgaag gtactggtgg tgatgtccag ccatcacagg
     3001 atctggtgcg cgttcttaat ctttgcgaca agctggcgca aaaacgaggt gataacttta
     3061 tctcgtcaga actgttcgtt ctggcggcac ttgagtctcg cggcacgctg gccgacatcc
     3121 tgaaagcagc aggggcgacc accgccaaca ttactcaagc gattgaacaa atgcgtggag
     3181 gtgaaagcgt gaacgatcaa ggtgctgaag accaacgtca ggctttgaaa aaatatacca
     3241 tcgaccttac cgaacgagcc gaacagggca aactcgatcc ggtgattggt cgtgatgaag
     3301 aaattcgccg taccattcag gtgctgcaac gtcgtactaa aaataacccg gtactgattg
     3361 gtgaacccgg cgtcggtaaa actgccatcg ttgaaggtct ggcgcagcgt attatcaacg
     3421 gcgaagtgcc ggaaggattg aaaggccgcc gcgtactggc gctggatatg ggcgcgctgg
     3481 tggctggggc gaaatatcgc ggtgagtttg aagaacgttt aaaaggcgtg cttaacgatc
     3541 ttgccaaaca ggaaggcaac gtcatcctgt ttatcgacga attacatacc atggtcggcg
     3601 cgggtaaagc cgatggcgca atggacgccg gaaacatgct gaaaccggcg ctggcgcgtg
     3661 gtgaattgca ctgcgtaggt gccacgacgc ttgacgaata tcgccagtac atcgaaaaag
     3721 atgctgcgct ggaacgtcgt ttccagaaag tgtttgttgc cgagccttct gttgaagata
     3781 ccattgcgat tctgcgtggc ctgaaagaac gttacgaatt gcaccaccat gtgcaaatta
     3841 ctgacccggc aattgttgca gcagcgacgt tgtctcatcg ctacattgct gaccgtcagc
     3901 tgccggataa agccatcgac ctgatcgatg aagcagcatc cagcattcgt atgcagattg
     3961 actcaaaacc agaagaactc gaccgactcg atcgtcgtat catccagctc aaactggaac
     4021 aacaggcgtt aatgaaagag tctgatgaag ccagtaaaaa acgcctggat atgctcaacg
     4081 aagaactgag cgacaaagaa cgtcagtact ccgagttaga agaagagtgg aaagcagaaa
     4141 aggcatcgct ttctggtacg cagaccatta aagcggaact ggaacaggcg aaaatcgcta
     4201 ttgaacaggc tcgccgtgtg ggggacctgg cgcggatgtc tgaactgcaa tacggcaaaa
     4261 tcccggaact ggaaaagcaa ctggaagccg caacgcagct cgaaggcaaa actatgcgtc
     4321 tgttgcgtaa taaagtgacc gacgccgaaa ttgctgaagt gctggcgcgt tggacgggga
     4381 ttccggtttc tcgcatgatg gaaagcgagc gcgaaaaact gctgcgtatg gagcaagaac
     4441 tgcaccatcg cgtcattggt cagaacgaag cggttgatgc ggtatctaac gctattcgcc
     4501 gtagccgtgc ggggttggcg gatccaaatc gcccgattgg ttcattcctg ttcctcggcc
     4561 caacgggtgt ggggaaaact gagctttgta aggcgctggc gaactttatg tttgatagcg
     4621 acgaggcgat ggtccgtatc gatatgtccg agtttatgga gaaacactcg gtgtctcgtc
     4681 tggttggtgc gcctccggga tatgtcggtt atgaagaagg tggctacctg accgaagctg
     4741 tgcgtcgtcg tccgtattcc gtcatcctgc tggatgaagt ggaaaaagcg catccggatg
     4801 tcttcaacat tctgttgcag gtactggatg atggtcgtct gactgacggg caggggagaa
     4861 cggtcgactt ccgtaatacg gttgtcatta tgacctctaa cctcggttcc gatttgattc
     4921 aggaacgctt cggtgaactg gattatgcgc acatgaaaga gctggtgctc ggtgtggtaa
     4981 gccataactt ccgtccggaa ttcattaacc gtattgatga agtggtggtc ttccatccgc
     5041 tgggtgaaca gcacattgcc tcgattgcgc agattcagtt gaaacgtctg tacaaacgtc
     5101 tggaagaacg tggttatgaa atccacattt ctgacgaggc gctgaaactg ctgagcgaga
     5161 acggttacga tccagtctat ggtgcacgtc ctttgaaacg tgctatccag cagcagatcg
     5221 aaaacccgct ggcacagcaa atactgtctg gtgaattggt tccgggtaaa gtgattcgcc
     5281 tggaagttaa tgaagaccgg attgtcgctg tccagtaatg ataaaacgag cccttcgggg
     5341 ctcgtttttg tctataagtt agacggaaaa gactatattt aagatgtttt gcctgaaaaa
     5401 tgagcgaacg ataaagtttt tatatttttc gcttgtcagg ccggaataac tccctataat
     5461 gcgccaccac tgacacggaa caacggcaaa cacgccgccg ggtcagcggg gttctcctga
     5521 gaatctcaac agagaaaagc aaaaaaatgc ttgactctgt agcggggaag cgtattatgt
     5581 acaccccgcg cagctgagaa aa
//
LOCUS       DADLFA010000214         5595 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000214 DADLFA010000000
VERSION     DADLFA010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5595
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.216"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..401)
                     /locus_tag="JQH65_003654"
     CDS             complement(<1..401)
                     /locus_tag="JQH65_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY6140928.1"
                     /translation="MGILEQEMKRLAQQAGGSHKTVHDRIKLVQRFCERLVLAQNVQI
                     RRVEQLKARYIEGYVRERLAQGITKRSLQNEMAAVRCILKQAGRDRLAQSERLNNRSL
                     GLSGASRNGTKRAITPEHYRHVLETARAKDPG"
     gene            complement(1266..1583)
                     /locus_tag="JQH65_003655"
     CDS             complement(1266..1583)
                     /locus_tag="JQH65_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016161430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAY6140929.1"
                     /translation="MDRGEIWLVSLDPTTGHEQSGKRPVLIVSKASFNTFTRLPVVVP
                     VTSGGNFAREAGFTVSLDGAGTKTTGVIRCDQPRTIDMGARSGKRLERIPDAVVNEVL
                     ARL"
     gene            complement(1584..1841)
                     /locus_tag="JQH65_003656"
     CDS             complement(1584..1841)
                     /locus_tag="JQH65_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAY6140930.1"
                     /translation="MYTTRLKKVGGSVMLAVPPAVLKTLGLSTDSEVGMTIDNGCLII
                     EPQKRPRYSLEELLAQCDPHAEISEEGREWIDAPAVGKEIL"
     gene            complement(2190..2396)
                     /locus_tag="JQH65_003657"
     CDS             complement(2190..2396)
                     /locus_tag="JQH65_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003842122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY6140931.1"
                     /translation="MMLQDWHPADIIAGLKKRGTSLSALSRQAGLASSTLANALTRRW
                     PKGERLIAEALGIKPEQIWPSRYR"
     gene            complement(2393..2854)
                     /locus_tag="JQH65_003658"
     CDS             complement(2393..2854)
                     /locus_tag="JQH65_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012822469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6140932.1"
                     /translation="MATIPTQTHPLLAVPFSAATDFTVLADHCENVAETLIESEDPVL
                     RLALCGRLAACLTLLQPTLNDPIPSHLTDSLTVDALPADMPRFVPDAELLCDYSLTLA
                     QLLIGQGMQPKVEQTITGLLCELVWYFAAEMKAPRWLRTADGVKFIDEVAA"
     gene            complement(3225..4829)
                     /locus_tag="JQH65_003659"
     CDS             complement(3225..4829)
                     /locus_tag="JQH65_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6140933.1"
                     /translation="MITKELVDESNFISQLVDIGRFDESYQQSVAFLNKLERITIRND
                     NYFIVLANIAGALVDIGQMQNNKNASELGCNLMEDNKEAFISVLGECLYYYNYGNALS
                     NLVSITNPNDHTFKSIEELVSLKNIYWRSFKLSAEEQEEFQAELSVNLANSLRSQFRL
                     SESLRYYDLTNLKGLDIPQAWVNRSMSLMVLNLISSSFSIKLLKEVRAGYIKASVSKN
                     IPPQWESFYLERIAQTNEKIAEYAVDDETDEHDEALTQQEFEVLSPYRQFCLRNHLTL
                     SEHGLYCPCVGSATDDLVIASRGGVTGDFIIPMEMVLNRLKSEFSLARHLYFDYLHPQ
                     NTDYIKYESHFLELYNDEVLGIEIEKIRTAFRLCFGILDKIAVAICELYNLYPTTKKG
                     TPQKNIYFQNFWQLDVDNRRQLFEKVKSPGLLALYSIATDLNKDKGGELAFYKEWRNG
                     LEHKFLVVHKSDKPEDIYESYQLIEDILFIKESDFIHHFEHLLQITRSAIFSFAFMVR
                     QEGMKEKKEGIHYMTRELHAINYSAD"
     gene            complement(4842..5207)
                     /locus_tag="JQH65_003660"
     CDS             complement(4842..5207)
                     /locus_tag="JQH65_003660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6140934.1"
                     /translation="MVSDKRADIIVSLPQIKIPIEIKRDYHRDVWTALNGQLDKLYTK
                     NPDAAGHGIYLVFWFGSARPNSLPHLAKNTSQPENASAMENMLNETVPVDKRDRLSAI
                     VIDVSCEGIPPVEAPKSSV"
     gene            complement(5235..5444)
                     /locus_tag="JQH65_003661"
     CDS             complement(5235..5444)
                     /locus_tag="JQH65_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001044500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6140935.1"
                     /translation="MNKTPANIMDLYSLLLDHIRDISNRIANENTDIYKQFWNEDSYG
                     RTLTPKPEESCRNIFLDLLRVRLNP"
BASE COUNT         1447 a         1346 c         1306 g         1496 t
ORIGIN      
        1 cccggatctt ttgcgcgggc ggtttccagc acatggcggt aatgctccgg cgtgatggcc
       61 cgcttcgtac cgttacggga tgcgccagac aggcccagcg agcggttatt cagtcgctca
      121 ctctgcgcca gcctgtcacg cccggcctgt ttcagaatgc agcgcaccgc cgccatctca
      181 ttttgcaggg aacgcttcgt gataccctgc gccagtcgtt cacgaacata tccctcaata
      241 taccgcgcct taagctgctc cacccggcgg atctgcacat tctgtgccag aaccagacgc
      301 tcacaaaacc gctgcaccag cttaatacgg tcgtgaacgg tcttatggct gccgcccgcc
      361 tgttgtgcca atcgcttcat ttcctgctcc agaatcccca tacattcccc ctttcatgat
      421 tgccgcaaac tcatccccaa aaactcaaca ctgatacagc gttccccggc gcacaggccg
      481 atagcccctg agttttgtcg gggctgcgaa cgatacctga gcgccggggc ggcagccgtt
      541 gaggtgtggt ggggcttcgg ttgtgctctc agagcagccg cccgcttgcg cgggtcatcc
      601 cccggatcgt ccagatccgc ctcggtatcg gttcatattc tccttatggg taaatggcct
      661 aaaaaggcgg tgtcagaatc cagccatgag ctggcgcagt tgagttttct ggtgcaggtc
      721 gaaagggctt gcgtgcgtca ctttcccgct ttgcgcggtt aaagtgacgc acggatctgg
      781 tgcagcatct gagatttatc gtctggtgca gttttacggt cacgcggcag caaagcctga
      841 cggcaatgca ggccgcagcc cgtaaggtaa aaggcgctga gggtgtcagc agatgcaggt
      901 tagccgggag aattccggcg cggtgcagat tcagaccagc aggctggcga agtaaaagga
      961 tgtatcgtat taaaactctg gcctccgtcg cgatgttctg cgacccggtg tttgcagagg
     1021 tctgcaccgg taaacggatc gaccgtcctg ataacaggcg cttctttcag gttgtgaagc
     1081 gttgggcgct gtctacgaca gcatagagca agggggagac tacgcctgtg ccgctggcgc
     1141 ggaaaggaga aactgttttt acctgcaaaa ggatttttta atcacttgtt gttataaaaa
     1201 atgagggcgc tgtatgtgac agcgccctcg ttcaggttac ataaaaaata tcagctcaga
     1261 atagcttaca gtcgagccag cacctcattc acgacagcat cgggtatacg ttccagccgc
     1321 ttaccgctcc gagctcccat atcaatggtt ctgggctggt cgcagcgaat aacgcccgtt
     1381 gtttttgttc ccgccccgtc cagtgaaacg gtaaaaccgg cttcgcgggc aaagtttccg
     1441 ccgctggtga cagggacaac aacaggcaac cgggtgaacg tgttaaacga tgccttcgat
     1501 acgatgagta ccggacgttt tccactttgt tcatggccag tggtgggatc cagtgaaacg
     1561 agccagattt ctcctctgtc catctacagg atctccttgc ccaccgcagg cgcatcaatc
     1621 cattcccgac cctcttcgct tatttcagcg tgcggatcgc actgcgccaa cagttcttcg
     1681 agcgaatagc ggggacgttt ttgaggttca ataatcaggc agccattatc aatggtcatg
     1741 cccacttcgc tgtccgtcga cagccctagc gttttcagca ctgcgggggg aaccgccagc
     1801 atgacggatc cgccaacctt tttcaggcga gtggtataca tagagcacct ccggatatta
     1861 tattttaata taacatccat gatagacgat gcacaactat tcatcagatt cattgtggtt
     1921 tccttagtgt aataaagcgt gaagaaagcc ttaatccatc ggaagacaga tccggcagtt
     1981 ttcaaaggcg acattgccgg taaaagggca accgtcctga tacatcaggc gcttctttca
     2041 ggtcatgaag catttcgtac aggcgggagc ttagtgcgta acaaagaagg ccgataatag
     2101 gaaactgctt ttgcagttca aaggattatg acggttcgga taaacaaaat gcggcgcagc
     2161 ttccgtatcc gggctgcgcc gcaggaacat cagcggtagc gcgaaggcca gatttgctca
     2221 ggcttgatgc ctaacgcttc ggcaattaac ctttcgcctt tgggccagcg gcgggtaagc
     2281 gcattagcca gcgtggagga cgccagacct gcctgacgcg aaagcgctga cagcgaagtg
     2341 cctcgttttt tcagcccggc gataatgtcg gcaggatgcc agtcctgcag catcatgcag
     2401 ccacctcgtc gataaacttc acgccatcgg cggtgcggag ccagcgcggg gctttcattt
     2461 cggcggcaaa ataccagacc agttcacata ataagcctgt tatcgtctgc tccacttttg
     2521 gctgcatccc ctgcccgata agaagttgcg ccagcgtcag tgagtaatca caaagtaatt
     2581 ctgcgtcggg gacaaatcgg ggcatatcgg caggtagagc atcaacggtc aggctgtcag
     2641 tcagatgaga cgggatgggg tcgttcagcg tgggttgcag caaagtgaga caggcggcaa
     2701 gtcgcccgca aagcgccagc cgcagcaccg gatcttcgct ttcgatcagg gtttcggcga
     2761 cgttttcgca gtggtctgcc agtacggtaa aatctgtggc agcactgaag ggaacagcaa
     2821 gtaacgggtg tgtctgagtg gggatcgtag ccataaaggc agcctccagt atgtggtttg
     2881 aaaaccacca ccagaggttc caatctcatg ggtggtggac tgaacgaggt tggaactacc
     2941 ggccatactg gaaccggcgc accttacggt gcccccgccc agcccaccat aattttggtg
     3001 tagctaagcg cgcgacataa aaaaagacgc agacgcgtca cgtgtcgcca gtatggttat
     3061 atttcggggt tccaatcccg gcacctgatt ttgcaggtgc gggtaaaaga taccgctttc
     3121 ggttgaaaac ggcaagggga agtcgtggaa tagagatcgc agattgttat aaatatacac
     3181 aatagttaat gttaacaaag acggcacata tattaacttt actctcaatc tgctgaatag
     3241 tttatcgcat gtaattctcg ggtcatatag tggataccct ccttcttttc tttcatcccc
     3301 tcttgcctca ccataaaagc aaacgaaaaa attgctgaac gagttatctg aagtaaatgc
     3361 tcaaaatggt gaataaaatc actttctttg ataaaaagaa tatcttctat tagttgataa
     3421 gattcgtaaa tgtcctcagg tttatcactt ttatgtacaa ctaaaaactt atgctctaaa
     3481 ccattgcgcc attctttata gaaagccaac tccccaccct tatctttatt caggtcggta
     3541 gcaatactgt acagcgccag aagccctggt gattttacct tttcaaataa ttgtcttctg
     3601 ttatcaacat ctaactgcca gaaattctga aaatagatat tcttctgagg tgtacctttt
     3661 tttgttgttg ggtaaagatt ataaagttca catatggcta ccgcaatttt atcaagaata
     3721 ccaaagcaaa gacgaaatgc cgtcctgatt ttttcaattt ctattcctaa aacttcgtca
     3781 ttatacaatt ccagaaaatg gctttcgtac ttaatgtaat ccgtattttg tggatgaaga
     3841 taatcaaaat aaagatggcg tgcgagtgaa aactccgatt taaggcgatt taataccatc
     3901 tccatgggaa tgataaaatc acctgttaca ccgcctcgcg aagcaatcac aagatcatct
     3961 gtagcactcc ctacacatgg gcaatacagg ccatgttctg ataaagtcag atgatttcta
     4021 agacaaaact gtcgatatgg gcttaacact tcgaactctt gttgtgtaag tgcttcatcg
     4081 tgttcgtcag tttcatcatc aacagcatat tctgcgattt tctcgttagt ctgcgctatg
     4141 cgttctaaat agaacgattc ccattgtggt gggatatttt ttgatacaga cgctttaata
     4201 tatcctgctc tcacttcttt tagtaacttg attgaaaatg aagatgaaat aagattgagg
     4261 accataagtg acatcgaacg gttgacccaa gcctgtggta tatctaatcc cttaagattg
     4321 gtaagatcat aatatctcaa tgactcactg agccgaaatt gacttcttaa tgaattagcc
     4381 aaattaacag atagttcagc ctgaaattcc tcctgctcct cagcagatag cttaaaagaa
     4441 cgccagtaga tattttttaa ggaaaccaat tcttctatcg atttgaatgt atggtcattg
     4501 gggttagtaa tgctcaccag attagatagc gcattaccat agttataata gtaaaggcac
     4561 tctcctaaca ctgatataaa tgcttctttg ttatcttcca tcaggttgca accaagctct
     4621 gacgcatttt tattattttg catctgcccg atgtcaacaa gagcacccgc aatgttcgcc
     4681 aatactataa agtagttatc gttcctgatt gttattcttt ccagtttgtt caggaacgca
     4741 actgactgtt gataagattc gtcaaatctt ccaatatcaa ccagttggct aatgaaatta
     4801 ctttcatcga caagttcttt tgttatcaaa ataaattttc cttatacaga ggatttcggt
     4861 gcttcaacag gaggtatgcc ctcacaggag acatctataa ctatcgctga taagcggtct
     4921 cttttatcaa ccggcacagt ttcattaagc atattttcca ttgcactggc gttttcaggt
     4981 tgagatgtat ttttcgccaa atgaggcagg gaattcggtc gagcagaacc aaaccaaaaa
     5041 accagataga taccgtgacc agcagcatcg gggtttttag tataaagctt gtccaattgc
     5101 ccattaagtg cagtccagac atcacgatga taatcacgct ttatctcgat aggtattttt
     5161 atctgcggta gtgaaacaat gatatctgcc cttttatcag aaaccatgtg tccttctggt
     5221 tcacagctta ttttttatgg attaagccgg acacggagta aatccagaaa aatattacgg
     5281 caactctctt caggtttagg cgttagggta cggccatagc tgtcttcatt ccagaattgc
     5341 ttataaatat cggtattttc attggcaatt cggttagaaa tatcccgtat atgatcaagc
     5401 aataatgagt aaagatccat tatgttggca ggcgttttat tcatcaatgt acttacagtc
     5461 tgcttccagt cggcatgatg gaactgtgat tctctataac gaattttctg attgctttgg
     5521 gcatgtgaga gatgatcccg ataactttca cattcaggca gcagtaataa gcgctcaagt
     5581 acttcactag catcg
//
LOCUS       DADLFA010000215         5439 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000215 DADLFA010000000
VERSION     DADLFA010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5439
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.217"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..914
                     /locus_tag="JQH65_003662"
     CDS             <1..914
                     /locus_tag="JQH65_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAY6140936.1"
                     /translation="PLSLRIGITSGIGLFIALMGLKNTGVIVANKDTLVMIGDLSSHG
                     VLLGILGFFIITVLSSRHFHAAVLVSIVVTSCCGLFFGDVHFSGVYSIPPDISGVIGE
                     VDLSGALTLELAGIIFSFMLINLFDSSGTLIGVTDKAGLIDGNGKFPNMNKALYVDSV
                     SSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTAVVVGVMFLLVMFFSPLVAMVPPYAT
                     AGALIFVGVLMTSSLARVNWDDFTESVPAFITTVMMPFTFSITEGIALGFMSYCIMKV
                     CTGRWRDLNLCVVVVAALFALKIILVD"
     gene            967..1419
                     /locus_tag="JQH65_003663"
     CDS             967..1419
                     /locus_tag="JQH65_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HAY6140937.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            1630..2820
                     /gene="nepI"
                     /locus_tag="JQH65_003664"
                     /pseudo
     CDS             1630..2820
                     /gene="nepI"
                     /locus_tag="JQH65_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
     gene            complement(2861..3154)
                     /locus_tag="JQH65_003665"
     CDS             complement(2861..3154)
                     /locus_tag="JQH65_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6140938.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            3376..4194
                     /gene="nlpA"
                     /locus_tag="JQH65_003666"
     CDS             3376..4194
                     /gene="nlpA"
                     /locus_tag="JQH65_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HAY6140939.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKITTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLHTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(4221..5120)
                     /locus_tag="JQH65_003667"
     CDS             complement(4221..5120)
                     /locus_tag="JQH65_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005030206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY6140940.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSLSPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRPEK"
     gene            complement(5231..5434)
                     /locus_tag="JQH65_003668"
     CDS             complement(5231..5434)
                     /locus_tag="JQH65_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000965477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAY6140941.1"
                     /translation="MLYFQDLMPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYH
                     ALFWLAIGMLGIAMICLLFIKDI"
BASE COUNT         1298 a         1238 c         1395 g         1508 t
ORIGIN      
        1 ttcccttaag tttacgtatt ggtatcacca gcggaattgg attatttatc gccttaatgg
       61 gattaaaaaa tactggcgtt attgtcgcca ataaagacac gctggtgatg attggcgatt
      121 taagttctca cggcgtgttg ttaggtattt tagggttttt tattataacc gtgttgtcat
      181 cacgtcattt tcatgccgcg gtgctggttt ctatagtggt gacgtcttgc tgtggattat
      241 ttttcggtga tgttcatttt agcggcgtct attccattcc gcctgatatt agcggcgtca
      301 ttggtgaagt agatttgagc ggcgcgttaa cacttgaact cgccggtatc attttctcct
      361 ttatgctgat caacctattt gattcatcag gaacattaat tggtgtaact gataaagcgg
      421 gcttaataga tggtaacggt aaattcccca atatgaataa ggcgctgtat gttgatagcg
      481 tcagttcggt ggcgggtgcg tttatcggca cctcgtctgt tactgcctat attgaaagta
      541 cttctggtgt ggcagtcggt ggccgcacgg ggctgactgc ggttgtggtt ggcgttatgt
      601 tcctgttggt tatgttcttc tcaccgctgg tggcgatggt tcctccttac gcaaccgccg
      661 gagcgttaat ctttgttggc gtgctgatga cttcgagcct ggcgcgcgtt aactgggatg
      721 attttaccga atcggtgcct gcgtttatta ccacggtgat gatgcccttt actttctcga
      781 tcaccgaagg gattgcactc ggctttatgt cgtactgcat catgaaagtg tgcaccgggc
      841 gctggcgcga tctgaacctg tgtgtggtgg tggtcgcagc tctgtttgca ctgaagatta
      901 ttctggtgga ttagtaactg gcgagggttt ccagatatca tgagttctga ttacgcagga
      961 gaactcatga tctggataat gctcgccacg ctggcggtag tgtttgtggt tggttttcgg
     1021 gtgctgacat ccggggccag aaaagcgatt cgccgtctca gtgatcggct gaatatcgat
     1081 gtcgtacccg tggagtcgat ggtcgatcaa atgggaaagt cagccggtga cgaattttta
     1141 cgttatttgc atcgcccgga tgagtcgcac ctgcaaaacg ccgcgcaggt gttgctcatc
     1201 tggcaaattg tcattgtcga tggtagcgaa cagaacctgc tgcaatggca tcggatttta
     1261 caaaaagccc gtcttgccgc gccgattacc gatgctcaag tcaggctggc gctgggtttt
     1321 ctgcgcgaaa ccgaacctga aatgcaggat attaatgctt ttcagatgcg ctataacgcg
     1381 ttctttcagc ctgccgaggg cgttcactgg ctgcattaaa accgcttcgt cttatcagcc
     1441 tacagaccgc gcctgacctg tcgggtggat aagacgttca cgccgcatcc ggtaatcttt
     1501 gccctgttcc gtttcgatct taactccagc tcaagcaaca ccgtaaaagt tgcaaaaacg
     1561 ttaaatgcgt cacacatttc aatgttaaag tggtcggctt ttcccctgaa acatgccacg
     1621 ggtaacacca tgagtgaatt tattgccgaa aaccgcggcg cggatgccat cacccgaccg
     1681 aactggtcag ccgttttctc ggtggcgttt tgtgtcgcct gtctgattat cgttgagttt
     1741 ttgcccgtca gtttgttgac gccaatggcc caggatttag gcatttcgga aggggttgcc
     1801 gggcaatcgg tgaccgtgac cgcctttgtg gcaatgtttg ccagtttgtt tattacccag
     1861 acaattcagg ctactgaccg ctgctacgtt gttattttgt ttgccgtttt gctgacgctc
     1921 tcctgcttgc tggtttcctt tgctaactca ttcagtttgc ttttaatcgg tcgtgcctgt
     1981 ctggggctgg cgctgggcgg gttctgggcg atgtcggcgt cgctgaccat gcgtctggtg
     2041 ccgccgcgta cggtgccgaa ggcgctgtcg gtgatcttcg gcgcggtttc tattgcgctg
     2101 gtgattgccg cgccgttggg cagtttttta ggcgagctta tcggttggcg caatgtcttt
     2161 aatgcggcgg cggtgatggg cgtgctgtgt attttctgga ttatcaaatc attgccttca
     2221 ctgccaggcg aaccctcgca tcagaaacaa aatactttcc gcttattaca acgtccgggt
     2281 gtgatggcag ggatgatcgc catcttcatg tctttcgccg ggcagtttgc tttcttcacg
     2341 tatattcgcc cggtgtatat gaacctggcg ggattcggcg tggatggctt aacgctggtg
     2401 ctgttgagtt ttggtatcgc cagctttatt ggtacgtcgc tttcgtcgtt cattcttaaa
     2461 cgttcggtaa aactggcctt agcaggcgcg ccgtgaatac tggctgtgag tgcgttggta
     2521 ctgacgttgt ggggaagcga taaaatcgtt gctaccggcg tggcgattat ctgggggcta
     2581 acttttgcat tggttcccgt cggctggtca acgtggatca cccgctcgct ggccgatcag
     2641 gcagaaaaag ccgggtctat tcaggtggcg gttattcagc ttgctaatac ctgtggcgcg
     2701 gcaatcggcg gttatgcgct ggataatatt ggtctgactt cgccgttgat gttgtccggc
     2761 acattgatgt tgctgactgc attgttggtt actgcaaagg tgaaaatgaa gaaatcctga
     2821 tatcaggtaa agaagcgttg gtcagtagtt gttgctgttc ttacatatcc gggcattcta
     2881 ccttacccaa cgcttcccaa taattgctct gattattacg ttccatcgcc acgatggcct
     2941 cgcgtatatg ttgtttattg ttttcatctg acaacattaa cttttcccac gcttcatctg
     3001 ataatgacgc ctgtggcgtg caaattttac gtaaatcttg taataccgtg gttttctctt
     3061 ttgcggactg cagtgagcta aaaggcgact cggcgtatac gatgcctggc atagcgaaaa
     3121 aagtgaaaat aagcagtagt gtcgttggtt tcatggattc aaccttttac aaatgtgtat
     3181 cgaatgaagc ataagtgtag tttgctttca tcgctctcat cattctgtac gaaacgcgct
     3241 tgcgtggtca gtaagaagtg ccagacttta tattccactt ttattccttt ttattcttat
     3301 cgatagcgtt tcgtttttta aaccgcagcg accttaccgc tatagtcagg taatcattaa
     3361 taaaaggata aaaaaatgaa actgacaaca catcatctac ggacaggggc cgcattattg
     3421 ctggccggaa ttctgctggc aggttgcgac cagagtagca gcgatgcaaa acacattaaa
     3481 gttggcgtta taaatggcgc agaacaagat gtcgcggaag tcgctaaaaa ggtggcaaaa
     3541 gagaaatatg gcctcgatgt tgaactggta ggatttagcg gttcgttgct accgaacgat
     3601 gcgacaaatc atggcgaact tgatgcgaat gtcttccagc atcgaccctt tcttgaacaa
     3661 gataatcagg cgcatggtta taaactggta gcggtgggaa atacgtttgt cttccctatg
     3721 gcgggttatt ccaaaaaaat cacaaccgtg gcgcaaataa aagaaggcgc gacagtggcg
     3781 atccccaacg acccgaccaa ccttggtcgc gcacttttac tgttgcaaaa agagaagcta
     3841 attactctga aagagggaaa agggttatta cataccgcgc tggatataac cgataatcct
     3901 cgtcatttgc agattatgga actcgagggg gcgcagcttc cgcgcgtact ggatgatccc
     3961 aaagttgatg tagcgattat cagcaccact tacattcagc agacagggct ttctccggtg
     4021 cacgacagcg tatttattga agataagaat tcgccgtatg tgaatatttt ggtggcacgg
     4081 gaagataata agaatgcaga aaacgtgaag gaatttctgc aatcttatca atcacccgaa
     4141 gtcgctaaag cggcagaaac catttttaac ggtggcgcgg tgcctggctg gtaattctca
     4201 ctcatgccgc gccggacgat ttatttttct ggccggcgac gggaatccat tgaaatcaaa
     4261 atcaccgatg acagaatcag cagcgttccc agccagtccg gtaatgtgaa cgtgatcccc
     4321 agcaacagca aagagagtag cgcgctactt aatggttctg cacagctcaa aatgctggct
     4381 tttggaccgc caattaattg tgctcctttc aggtacagac taaatgtcag ggacgtacca
     4441 atgaccacca aataaaaaaa cgccagaatc aaactgccgt taacgacaaa gtttgttcct
     4501 tgtctggcat aaaaaggcaa cagaatcaga ccgccaatca gcatactcca gccgacgact
     4561 ggtaacgtgc catagcgggc aattagcgtt gagggatagg tggtatagaa tgcagcagca
     4621 aatgccgagg caatgcccca gaacaacgcg gcaggagaga gcgataatga cgtcggatta
     4681 ccgtgtgtca ccaataaaaa agtcccgacc agcgatgtca aaatagcgca gaaaaccaga
     4741 atgcccgggc gcgatttacg caccagtgag aaccaggcga cgataatcgt cggtgagagg
     4801 aattgcagca ccgttgccgt ggctgcgttc gatttttcga tggttagcaa aaaagtgagc
     4861 tgtacagtta gcgcgccaac cacggaaaaa atcagcaggc taatggcatc tttatgattg
     4921 ttaataatag aaaagatttt atcgccatga acaaatgaca gcgtcagtag aatcaaaccg
     4981 gcgaatatca aacgcgtcat agtcaaaaac tgcgacgaca tctggctttg ctccatgatg
     5041 tattgcgcgc agacccctga acttccccac aacacggcgg caatcagaac gttcagcatc
     5101 ccctttctgg tggaacccat ttttccctct gcatactgtt ttgtcgttta tgtatgcagg
     5161 catgatagca aaaatggcga ggatggctct gtttgcgcca cgccgatttt tcttagccca
     5221 gactggtgag ctaaatatct ttaataaaca gcaggcaaat catcgcaata cccaacatcc
     5281 ctatcgccag ccagaacaac gcatggtagc tccagatttc aaccataatt ccgtcaacag
     5341 agccggcgat aatccagccg acgcgtgaag tatttgcata taacgttgtc gcagagccta
     5401 ttttttcagg cattaagtcc tgaaaataaa gcatgccga
//
LOCUS       DADLFA010000216         5336 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000216 DADLFA010000000
VERSION     DADLFA010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5336
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.218"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(889..1830)
                     /gene="fabY"
                     /locus_tag="JQH65_003669"
                     /gene_synonym="yiiD"
     CDS             complement(889..1830)
                     /gene="fabY"
                     /locus_tag="JQH65_003669"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HAY6140942.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1875..2312)
                     /gene="dtd"
                     /locus_tag="JQH65_003670"
     CDS             complement(1875..2312)
                     /gene="dtd"
                     /locus_tag="JQH65_003670"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAY6140943.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(2309..3182)
                     /locus_tag="JQH65_003671"
                     /pseudo
     CDS             complement(2309..3182)
                     /locus_tag="JQH65_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
     gene            complement(3176..3775)
                     /gene="yihX"
                     /locus_tag="JQH65_003672"
     CDS             complement(3176..3775)
                     /gene="yihX"
                     /locus_tag="JQH65_003672"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HAY6140944.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRVPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVELRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3916..4803)
                     /locus_tag="JQH65_003673"
     CDS             complement(3916..4803)
                     /locus_tag="JQH65_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HAY6140945.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQAGPWD
                     DCFGFYNGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            complement(4793..>5336)
                     /locus_tag="JQH65_003674"
     CDS             complement(4793..>5336)
                     /locus_tag="JQH65_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HAY6140946.1"
                     /translation="GIVYISSQLVLSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQK
                     SLYLSGAGFCVLFAFPFFLLLDSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGT
                     KVRYTGLSFAYQFSAILGGLSPLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIE
                     PTDEQETASYRYIREQSHEN"
BASE COUNT         1441 a         1345 c         1252 g         1298 t
ORIGIN      
        1 gataaatgaa gaaaaaccgt attcatcgcc attaccacca ccttacctat attactcatt
       61 aatgtatgtg cgaaaattac gccggaaaat cattaaaaaa aagcggattt ttgcatgttg
      121 ttttgttttt gcgaggcgtt ttccagaaat aataagagtg ttgcagttgt ttcatttatt
      181 actcaaaaat ggtttagtta tcgcccagga tgaattatgg gtgctatgaa tatattatat
      241 tcagagatat ttatttgcat aatctgaatg gtgtagccga aaaacgtcag catcctaaca
      301 gcaccaggat ttaaggtgaa attaatcttt catcacagac cggtcagtac tgccaccaac
      361 cggtctttac ttgctcatcg ttataatttt gttggtaaca gttcgggaaa gttgtttttc
      421 taaattaaca acagaataac tttcttgttt aatgagatta atattggcaa ccattatagc
      481 ctccttccca cggtttaaga gcaaacatac aacataatga ttcataacat acagagcagt
      541 aaatgaatga tactgacaat gatgccagcc cattatcttt gcataatgtt tgtgagctat
      601 agattttaaa aatatttatg aggaaagtat ggcaccagag gaggttttga ctatcaaaaa
      661 tcgctcaaac ctgaactaag atgatgagtt aagcattatc tgcgataatt gccactcagc
      721 tctacaaata atatttaaat gctcctgaac agtgatgtag caagacacgt atggtgccaa
      781 cgaagaataa aaatttatag agttcttatt caatattacc ggcatagaca gggaatgatg
      841 taaatgaccg gaatgaatga gagaggcagc accgcccctc tccataagct actcttcttc
      901 gttcccgccc tcttcatacg ggccaaatgg cttcgcgggc agaacgatat acgtgccttc
      961 aaacactgca cccggcgtct cgtcgccaaa gatttcaact tgcatctgca cccgtgcttt
     1021 tcgtccgcgc gccagacggt cgagatcgcc gcttaaggca ccaaggtcgg ctaccgcatg
     1081 aggtttaccg ctaatcggct tgctgtagcg gatatgcgca tcggccagaa taatcgttcc
     1141 gccgaggtgg cgttcgcgca gcatcagcca gataagcccc caaccggtga gcgttgccag
     1201 cgagaataaa ctcccggcaa acagcgtatg gtgcggattc tgattaccgg tttccggcat
     1261 ggtggtgata aatttttgcc cggtatattg ctgaatgcgc acgcccattt tttcactaag
     1321 cgggatgtgt tcgtaccacg cctgttgcag ctgcgcgcac cagtcgccgc gatgcaaaat
     1381 atcatccaga gtggcaacag gtttaatcat caaaaaatgg cgaatcggcg tggtggttgg
     1441 tgtggtgatt tctccctgat taacaaaccc cagcttggcg aaaaactcca ccgcgtcttc
     1501 acgggcgcta caggtcacgc gcttaacgcc ttcctgacgc gccaccgact ccagggtcat
     1561 cgccatcaac gtccccagcc ctttgtcctg cacgtcggga tgaacggcca taaagcgaat
     1621 ggacgcttca ttgtcggcat taatatacag tcggcctacc gccaccagat tcccctgctc
     1681 gtcgacgacc atctgatgat gcgccatcgc atcccacgcg tcgcgttccg aaccttttgg
     1741 ttgatgcagg ggcttacgca acatttccca gcgaaactga tagtaacgct ctaactcttc
     1801 ttctgtttgt ggaacccgaa ggtgatacat agcgatactc tctcttgttt cccgtgacaa
     1861 ccctggaagc tggctcatac ctgcaaccag aatgtcacgg ggccatcatt gaccagcgat
     1921 acctgcatat cggcagcgaa gcgtcctgtt tgcgtgttca tctcttgctg gcggcagcgt
     1981 tcgacgaaat agtcatataa cgcctctgcg cgatccggtg atgcgccttt ggagaaactt
     2041 gggcgcatcc cccgttcggt atctgcggcg agggtaaact gggaaaccac cagtacactg
     2101 ccgcccgcct gttgcacgtt gagattcatc ttgccttcgg catcgctaaa gatgcggtag
     2161 ccgagcacac gctcgcacag acggtttgct ttctgttcgt cgtcatcctt ttcgacaccc
     2221 aataacacca aaagtcccgc gccaatttcg cccgtcactt ctccctccac ggtgacgctg
     2281 gcacgggtta cgcgttgaat taatgcaatc atggttcgtc gtcttcttct tgttcagctg
     2341 cttgttttag tttgcggtat tccccgagag tgacagtaat ttccgcgcca agcaagacga
     2401 tacaccatgt ccagtagacc caaacaaaga gaatggggat caccgccagc acaccgtaaa
     2461 tgagctgata tgacgggaac atggtgatat aaagcgcgaa acctttcttt cctgcttcga
     2521 acaggagtgc ggcgacaaac gcgccgacaa tcgcgtcgcg gttaggtacg cggatggtag
     2581 gaacaatgct gtacagcaac cagaaggaga tccacgacaa cagcaacgga aaaatacgca
     2641 gcacgttgtc gatgacagta ttgagatcgc tcgcccagcg cagggagagc aaataggaac
     2701 tgatcgccag actggcccct gccagcagcg gccccagcgt taaaatcatc cagtacacgg
     2761 cgaacgagta aatttttggt cgcgctcgtt tactgcgcca gatggtattc aacgcgctat
     2821 cgatggagta catcaacaat aacgcggtga cgatcagccc gcacgcccca acggcggtca
     2881 tcttgttgga attggcaaca aattgttcga tataccgctg aataacatcg ccagtagcag
     2941 gcagaaagtt ggcaaaaata aagtgacgta actgaatgct gacgtcggaa aacatgggaa
     3001 aagcggcgaa aagcgcaaaa acaacggcaa ccagcggcac taatgagagc aacgaccaca
     3061 taggcaaggt tacctgccag ggttgtcatg ttgtcctcat caatacgttg ccagagtagt
     3121 tttagccagg cccatagtgg acgggtacgg tgcctggctt tgtcctgaat ggtttttagc
     3181 ataacacctt cgcgaaatag tccgggatgg tggttttatc tttcaccaga atactggtaa
     3241 tgcccagctg attggctcct tctatattat cggcgttatc gtcgaaaaag accgtatcgc
     3301 tgggtgaaaa accttccgcc tgcaaaacat gctggtaaat tcgtgcttca ggtttgcgca
     3361 tccccagatc ttgcgacaga tagatatggt cagcagcatc acgaatttcc gggtattctt
     3421 ccggccagaa ggtggtatgc aggcggttgg tattggaaag caccaccacg cgatgcccct
     3481 gctcacgcag tttatgcatg atggcgatca cttccgggcg cagctcaaca aacaccgcct
     3541 gccagccgtg agagaactgc tcgtagctta gcggtagagc catctcatga cacagcgcct
     3601 ctgcgaacgc ttcgtcgcta atttccccac gctcatgctg atgaaacgcc tcccccatat
     3661 gaaaactctt cttaagcgta gccagtggaa cacgcgttaa atcgctccag gctcccagca
     3721 cacggttaaa gtcgatatcg acaatcacat tacctaaatc aaagatatag agcatttttg
     3781 cctcctttcg cgccacaaga aattaactgt agcgggaaag gatgggtttg gctatgtggc
     3841 aataagggta atcttgtgat gctgattgcg aaaaagcaga gtaatttcac tggctttttc
     3901 gcaatagatg aatggttaag attcaggtgt aaattgccaa cgagtttcga tgaccagcgg
     3961 cctatctggt gtcaccaatt ccggcgttag atttattgcg ttaggtgcct gagtcagcgg
     4021 gttaatacag gtggcatcgg gctgtttatc aaataccacc agactgtttg cagaagagat
     4081 catggtcatc gcgagtttac caggccacag taacttaacc ttcaccccgt tataaaaacc
     4141 aaaacagtca tcccagggac cggcttgagg agagatacga tttccagttg gcagttcatc
     4201 actacccgct tcttcttgcc agtccgcatc aaaaagcacc tgcaacgatt ctgtattttg
     4261 cggagtcagc tttttcgcaa accacggatg ccagccagcg gaggccggga atgtatcggc
     4321 atatgaatgg acttccagtt gcaaaaccag cgcatcgttc tccagcaaaa aagtctgaat
     4381 cacttcccct tgccagggcc acggtgaagc caggggcata cgtaaagtca gggaatctgc
     4441 tgttttgtcg atgatttccc acgttgagta acaagccata ccatgcaacg catgtggtgg
     4501 tttattggcg ggtaaagaat aacattgccc accagcattc agcgtcgcat tccccagtcg
     4561 ccctgcccac ggcaccattg ggaaacagcc atactgaaaa gctctgcgct gcggctgcca
     4621 ttggcgtaat acttcagaac caaatgcctt taacgaggtt atcctggcac catcatgcgg
     4681 ataaatctgc aggtgaatat tgcctttttc cagcgtcact aactctccta caggcccagg
     4741 ccatggcgcg tcaaacgcct gttgtagcgc caggtaatgc tcgttttttc gttcagtttt
     4801 catgagattg ttccctgatg tagcggtaag aagcggtttc ttgttcatcg gtcggctcaa
     4861 tcagccagac gcaaacaaag gaaagaactg agacagcaaa taaaaacaga gcgacatacc
     4921 acggttttcc tccccctagc gccagcaaac ttgaggcgat aagggggctt agtccgccaa
     4981 gaatcgcgga aaattgataa gcaaaagaaa ggccggtata tcgtactttg gtaccgaaca
     5041 tgcgggtgaa aagtgttggc tgcacggcaa acatcatcgt cggacctaag ttgtagccga
     5101 ggatcatact gcaccagatg atgagcgtac ttttgctatc cagtaataag aagaaaggaa
     5161 acgcgaacag gacacaaaat cctgctcccg acaaatacag acttttttga cctatacgat
     5221 ctgataacca tccaaccaat ggacaactga aaataccgat cgccgaggca atcaacatac
     5281 ccgtcagggg aatatctcgc gacaatacta attggctgga aatatagaca atacca
//
LOCUS       DADLFA010000217         5219 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000217 DADLFA010000000
VERSION     DADLFA010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.219"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..1113
                     /locus_tag="JQH65_003675"
     CDS             <1..1113
                     /locus_tag="JQH65_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAY6140947.1"
                     /translation="VKVTADEVNKIMQAANISQPDADKLLAVSRGEFVEGISDPNDPK
                     WVKGIQTRDSVNQNQHESERNDQKAEQNSPNALQNEPETKQSEPVAQQEPEKVCTACG
                     QSGGGNYPDCGAVMDDATYQKTFDEENQVEVQENDPEEMEGAEHPHKENAGSAQDHAS
                     DSKTGETADPLITVNGHHEITSTSRTCDHLMIDLETMGKNPDAPIISIGAIFFDPQTG
                     DMGPEFSKTIDLETAGGVIDRDTIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDE
                     NSGEFFVQVWGNGANFDNTILRRSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDART
                     AIPFEGERHNALDDARYQAKYVSAIWQKLIPSQADF"
     gene            1172..1375
                     /locus_tag="JQH65_003676"
     CDS             1172..1375
                     /locus_tag="JQH65_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HAY6140948.1"
                     /translation="MSSLFLTEDELLILTGCKYASHQRKWLMENELPFYTNRSGKPIV
                     SRDLFTCNKTLPPREVEPNFGAI"
     gene            1555..2400
                     /locus_tag="JQH65_003677"
                     /pseudo
     CDS             1555..2400
                     /locus_tag="JQH65_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142672.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(2428..2517)
                     /locus_tag="JQH65_003678"
     tRNA            complement(2428..2517)
                     /locus_tag="JQH65_003678"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2481..2483),aa:Ser,seq:cga)
     gene            2611..3408
                     /gene="mtfA"
                     /locus_tag="JQH65_003679"
     CDS             2611..3408
                     /gene="mtfA"
                     /locus_tag="JQH65_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HAY6140949.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            3509..3584
                     /locus_tag="JQH65_003680"
     tRNA            3509..3584
                     /locus_tag="JQH65_003680"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3542..3544,aa:Asn,seq:gtt)
     gene            3871..5007
                     /locus_tag="JQH65_003681"
     CDS             3871..5007
                     /locus_tag="JQH65_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6140950.1"
                     /translation="MLARSGKVSMATKKRSGEEINDRQILCGMGIKLRRLTAGICLIT
                     QLAFPMAAAAQGVVNAATQQPVPAQIAIANANTVPYILGALESAQSVAERFGISVAEL
                     RKLNQFRTFARGFNNVRQGDELDVPAQVSEKKLTPPPGNSSDNLEQQIASTSQQIGSL
                     LAEDMNSEQAANMARGWASSQASGAMTDWLSRFGTARITLGVDEDFSLKNSQFDFLHP
                     WYETPDNLFFSQHTLHRTDERTQINNGLGWRHFTPTWMSGINFFFDHDLSRYHSRAGI
                     GAEYWRDYLKLSSNGYLRLTNWRSAPELDNDYEARPANGWDVRAESWLPAWPHLGGKL
                     VYEQYYGDEVALFDKDDRQSNPHAITAGLNYTPFPLMTFSAEQR"
     gene            5011..5219
                     /locus_tag="JQH65_003682"
                     /pseudo
     CDS             5011..5219
                     /locus_tag="JQH65_003682"
                     /inference="COORDINATES: protein motif:HMM:NF023351.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1493 a         1206 c         1251 g         1269 t
ORIGIN      
        1 gtaaaagtca ccgctgacga agtaaacaaa attatgcagg cagccaatat cagccagcct
       61 gacgccgata agttacttgc tgtatcgcgt ggtgaatttg ttgaggggat tagcgaccct
      121 aatgatccga aatgggtcaa ggggatccag actcgcgatt ctgtgaacca gaaccagcat
      181 gaatcggaac ggaacgacca aaaagcggaa caaaacagcc caaatgcgtt acaaaacgag
      241 ccagaaacga aacaatccga accagtagcg caacaggaac cggaaaaagt ctgcaccgcc
      301 tgcggtcaga gcggtggcgg caactaccct gattgtggcg cggtgatgga cgacgcaaca
      361 taccagaaaa cattcgatga agagaatcag gttgaagttc aggaaaatga tccggaggaa
      421 atggaaggcg ctgaacatcc acacaaggag aatgctggca gcgctcagga ccacgccagc
      481 gatagtaaaa ctggcgagac ggcagatccc ttaattacgg tgaatggtca tcacgaaatc
      541 acatccacca gcaggacgtg tgaccatcta atgatcgacc ttgaaaccat gggaaaaaat
      601 cctgatgccc cgatcatctc aataggtgca atatttttcg atccgcaaac cggagatatg
      661 ggacccgaat ttagtaagac tatcgatctg gaaactgctg gcggagtcat tgatcgggac
      721 accattaaat ggtggcttaa gcaatcacgc gaagcgcaat ctgccattat gaccgatgaa
      781 atcccgttag atgatgcact gttacaattg cgggaattta tcgacgaaaa ctccggtgaa
      841 ttttttgttc aggtctgggg aaatggagcc aacttcgaca acacgatttt gcgccgttca
      901 tacgaacggc aggggatccc ctgcccgtgg cgttactaca acgatcgcga tgtacgcaca
      961 atcgttgagc tggggaaagc catagacttc gatgccagaa cggctattcc attcgaaggt
     1021 gagcgccata atgcacttga tgacgctcgt taccaggcaa aatacgtttc agctatctgg
     1081 caaaaactga tcccgagtca ggctgatttt taatgttcaa ccctaattgc cgctaaccgt
     1141 atatagttag cggcggttat gagatatagc tatgagcagc ttatttttaa ccgaagatga
     1201 attgctaata ttaacgggct gcaaatatgc aagccaccag cgaaaatggt taatggaaaa
     1261 cgagcttccg ttctatacca atcgtagtgg caaaccgatt gtcagccggg atctatttac
     1321 ctgcaataaa actttaccac cacgcgaggt agagccgaat tttggtgcga tctgatggga
     1381 agacgaagga aaaatcctga gcacgaaaaa ttacctccaa atgtataccc aaataaatat
     1441 agttatgtat ggaaaccaac atccagcgaa tctgtaacac taaccgccat caaggatggt
     1501 ttagctgctt tatggaaaaa gtatgaggaa actgtaaata atcgcgatcg tgcaatgaca
     1561 ttcggtcgct tgtgggaaaa attcctcgcc agcgcctatt acagtgacct cagtccaaga
     1621 acacaaaaag attatctgca acatcaaaaa agttgcttgc cgtattcggt aaggtgccgg
     1681 cagattccat aaaaccagaa cacatccgtc gatacatgga caagagaggg gaacagagta
     1741 aaacgcaagc caaccatgaa gtcccgcgtt tacagttggg ggtatgagcg agggtacgtg
     1801 aaggctaacc catgtgcagg tgtaagtaaa ttcaaggcca aaaaccgcga acgatatgta
     1861 accgacaaag aataccaggc agtattaagc gttgcacctc ttcctgtttt tatcgcaatg
     1921 gaaattgcct atctgtgtgc agcgagggtt tccgatgtgt tatcgctgaa atgggagcag
     1981 attggaaacg acgggatctt tatccagcaa gggaaaacag gaaaaaaaca gataaaagca
     2041 tggagtccac gattacaggc ggcgatcgaa aaagcaaaac agttaccaac atccgcctat
     2101 gtaatcagca atcaatacgg caaccgatat atgtacaaag gctttaacga aatgtgggta
     2161 gaagcaagaa atcgcgcagg caaaatttca ggtattttaa ccgacttcac ctttcatgat
     2221 ctgaaggcga aaggaatttc agactatgaa ggaagcagtc gggataagca acttttctct
     2281 ggtcacaaaa ccgaggggca agtgctaatc tatgacagga aggttaaagt ttcaccgaca
     2341 cttgatgtcc cgttacctga aaatattcca agtaattcca cgtgtgattt ttgtcactga
     2401 cttaatgatg tataagtgat tgaattttgg cggagagagg gggatttgaa cccccggtgg
     2461 agttgccccc actccggttt tcgagaccgg tccgttcagc cgctccggca tctctccgtt
     2521 cagatggttg ccatgatgcc aggaaatttg gcattttaac agtccctgtc cgtgcaattt
     2581 tgttcaagtg acgagtttgc gagcaaaacg atgattaagt ggccctggaa agtacaagaa
     2641 tcagcacatc aaactgccct tccctggcag gaagcactat cgatccccct tttaacgtgt
     2701 ctgacggaac aggaacaaag caaattagtc actcttgccg aacgtttttt acagcaaaag
     2761 cggcttgttc ctttacaggg ctttgaactg gattcattaa gaagctgccg gatagcactt
     2821 ctattttgcc tacccgttct ggagttagga ctggaatggc tggatggttt tcatgaagtc
     2881 ttaatttatc ctgcgccatt tgtggtcgat gatgaatggg aagacgatat cggtctggtg
     2941 cataaccaac gtattgttca gtcaggtcag agctggcagc aagggcctat cgttttgaac
     3001 tggttggata tacaagattc ttttgatgct tctggtttta acctgattat tcatgaagtc
     3061 gctcataagc tggacacccg taacggcgat cgcgccagcg gagttccctt tattccgttg
     3121 cgtgaggttg ctggctggga acacgatctt catgctgcaa tgaacaacat tcaggaagaa
     3181 atcgaattgg ttggcgagaa tgcggcgagc attgatgctt atgcagccag cgatcctgct
     3241 gaatgttttg ccgtactttc tgaatatttc tttagcgccc cagaactttt tgctcctcgt
     3301 ttcccttcat tgtggcaacg tttctgccaa ttttatcaac aagatccttt gcagagactg
     3361 catcacgcta atgatacaga ctcgttttcg gcgacgaatg ttcattaatt aacaactttg
     3421 catattaatt aaccaattga aatgacttat gaaatttagt gttgacagac aaggtaccgc
     3481 taagtaatat gcgccccgtt cacacgattc ctctgtagtt cagtcggtag aacggcggac
     3541 tgttaatccg tatgtcactg gttcgagtcc agtcagagga gccaaattct aaaaattcgc
     3601 ttttttagcg caatgtcact gaccttagtt gaacattgtt ttttaacgga tagcggcttt
     3661 ttaacatctt aagcgccctc gacctttatg gttgagggcg ttttgctatg aacgccatca
     3721 ccattttccc ctcgattata aaacttgagt tattcagtag tctcccctct tgcaactcac
     3781 acccaaaact gcctaacgaa aagttattaa ttttcaatca tattgctatc agtatttaca
     3841 ttttttcgct gtgctagaaa gggcgcattt atgttagctc gttcagggaa ggtaagcatg
     3901 gctacgaaga agagaagtgg agaagaaata aatgaccgac aaatattatg cgggatggga
     3961 attaaactac gccgcttaac tgcgggtatc tgtctgataa ctcaacttgc gttccctatg
     4021 gctgcggcag cacaaggtgt ggtaaacgcc gcaacccaac aaccagttcc tgcacaaatt
     4081 gccattgcaa atgccaatac ggtgccctac atccttggag cgctggaatc ggcccaaagc
     4141 gttgccgaac gtttcggtat ttcggtggct gagttacgca aactcaacca gtttcgtacg
     4201 tttgctcgag gttttaataa tgtccgccag ggtgatgaac tggatgtccc ggcacaagtt
     4261 agtgaaaaaa aattaacccc gccgccgggt aatagcagtg acaacctcga gcaacagata
     4321 gccagtactt cacagcaaat cgggtctctg ctcgccgaag atatgaacag cgagcaagcg
     4381 gcaaatatgg cgcgtggatg ggcctcttct caggcttcag gcgcaatgac agactggtta
     4441 agccgcttcg gtaccgcaag aatcacgctg ggcgtggatg aagattttag cctgaagaac
     4501 tcccagttcg attttctcca tccgtggtat gaaacgcctg ataatctctt tttcagtcag
     4561 catactctcc atcgtactga cgagcgtacg cagattaaca acggcttagg ttggcgtcat
     4621 ttcactccca catggatgtc aggcatcaac ttctttttcg accacgatct tagccgttac
     4681 cactcccgcg ccggcattgg cgcggagtac tggcgcgact atctaaaatt aagcagtaac
     4741 ggctatttgc gactgaccaa ctggcgcagc gcacctgaac tggacaacga ttatgaagca
     4801 cgcccggcca atggctggga tgtacgcgca gaaagctggc tacccgcctg gccgcacctt
     4861 ggcggtaaac tggtctatga acagtattat ggcgatgaag tggccctgtt cgataaagac
     4921 gatcggcaaa gtaatcctca tgccataacc gctggactta actatacccc cttcccgctg
     4981 atgaccttca gcgcggagca acgctagggt aaacagggcg aaaatgacac ccgttttgcc
     5041 gtcgatttta cctggcaacc tggcagcgca atgcagaaac agcttgaccc gaatgaagtc
     5101 gctgcacggc gtagccttgc aggcagccgt tatgatctgg tggatcgcaa caacaatatc
     5161 gttctggaat atcgcaaaaa agaactggtt cgcctgaccc tgacagaccc cgtgacagg
//
LOCUS       DADLFA010000218         5208 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000218 DADLFA010000000
VERSION     DADLFA010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5208
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.220"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..117
                     /locus_tag="JQH65_003683"
     CDS             <1..117
                     /locus_tag="JQH65_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HAY6140951.1"
                     /translation="DWDKEQLQDANSSWMVEDSFPRALREYNEMVDDYNSLR"
     gene            complement(167..1915)
                     /locus_tag="JQH65_003684"
     CDS             complement(167..1915)
                     /locus_tag="JQH65_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAY6140952.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISASGSAGYQLEIFERRSFFQLLQKHDNDYRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERACCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EQSGMFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPDIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDNDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLNCRVIIVRSLSELVAIREE
                     IEPLLIINNSHYLLEDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASILCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(1915..2985)
                     /locus_tag="JQH65_003685"
     CDS             complement(1915..2985)
                     /locus_tag="JQH65_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAY6140953.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQRQRPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQMD"
     gene            complement(2975..4426)
                     /locus_tag="JQH65_003686"
     CDS             complement(2975..4426)
                     /locus_tag="JQH65_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HAY6140954.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFTGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEMQSGSVMSHLMAGISAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            complement(4437..4883)
                     /locus_tag="JQH65_003687"
     CDS             complement(4437..4883)
                     /locus_tag="JQH65_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAY6140955.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHKEFIHQLQQGDTDQVLALLNQTLSS"
BASE COUNT         1319 a         1341 c         1315 g         1233 t
ORIGIN      
        1 gactgggata aagagcaact tcaggacgca aactcaagct ggatggtcga agactctttc
       61 ccgagagcat tacgcgagta caacgaaatg gttgatgact ataatagcct gcgttaaacg
      121 tttttgatga taaatgcagt gtcggatgcg gcgcatctga cacagttcac cccttgtgaa
      181 gtaactccat aaatgcctca tatcctgtta acccggcaat aacctctgcc ggatgctgac
      241 acaatatgct tgccagatag ctaaatattt ttagctcatg acgcgcatcg gcggcggcgc
      301 aggcgatcaa gacatggtta atgacttcgt tattcacctc aactggttgg gcgagggtaa
      361 taaaaaatcc acgaaaacgt cgctcctgtt cgctccagca atgtgggatg gcgaggcgat
      421 taacaatcag gttttcacct tcgccttcgc gggcgatgat gcgttgtgct tcatcggcgg
      481 taatatggtg ttgtgcgact aattgcgcac aaatttgccg ggtaatatgt tgccagcttt
      541 caccgcgtac attcgaataa tgaaaacttc cgggggcaga gaaaaaacgc tccggttgct
      601 ggcgaataaa tgccgtcgcc agaaaatgtt ttatttgttc gataccggca gccgtaatga
      661 tattttttac ggtgatgtaa ttatttaccg cgtcttccag taaataatgg ctattgttaa
      721 tgatcaataa cggctcaatc tcttcgcgaa tggcaacaag ttcgcttaag ctacggacaa
      781 taattacccg acaatttaaa acatcgcgct caatggcgag ctgattaata gtggcaatcg
      841 cgttctggtc cgagagcaaa atgatcggct ggcgttcgtt ttgatgccgc tccagcgcac
      901 aggcaaaata caaaccgatc agatcgttgt cgaaaagggg aatatcgagc tgttcacgca
      961 gtagcgtaat aaagtgcaga ctcatatcaa aggccgcagg ccaggctgct ttcaggttat
     1021 tcatgctact ttggcggtgc tcagcaatcc acaccggtgc ggcaaggcaa cgtaataaat
     1081 gcccggtaat attctcaacc aactgcgtat caataatatt caatgacgcg atgtctggca
     1141 ccctttgcag caaccccagc acatgatcgg ctgaaattac ctgcgcttgc tgatgctgtt
     1201 tctctatcaa acctgtcagg gttcttacag cgttatcacc aagaaacatg ccgctttgtt
     1261 caacgatttg ttttatctcg tcaccgggat attgcggcca ctcatcggtc agttgattac
     1321 gcagggcgta aatcgccaga atcaggctgg agagataatc accctgcatg agcggccagc
     1381 ggtgttggtt gtcgcaggcc gtggataaat gctgaaggtt gtctcgtgtt acgcccgcca
     1441 gcggaattaa aaacggatct ttgcgcagca agttcgccag caagataacg cgcttctctt
     1501 ctgtctcatc aatgaaatgg cccaaaccgg ggcggctggc caggcaacaa gcgcgttcat
     1561 aacgctgttt taaccggggc agacgctctg cgacccacgt ttctggcaaa ttaagcgccg
     1621 aggcgagttg cgcacgggga gtaaaggtat tcagtaataa cagtgccagc agccgatagt
     1681 cgttatcgtg cttttgcagc aactgaaaaa agctgcggcg ctcgaagatt tccagctgat
     1741 agcccgcact accactggcg gaaatgcggg ctttgccgtt aagggtaaag ttgagatagt
     1801 caatatcacg caggatggtc ctgcctgaaa cgccagtctg ttgcgccagc tcaccaggcg
     1861 tgcgcggctg ttgctccagc agatcgacaa tctttaactg gcgttcgttt agcattaatc
     1921 catctgacgg aactggctaa acgcgttgac tttctccgcg gttaaggtgg tcagaaaatc
     1981 ccgtatcaac gtgagcagag catcgtaatc ggcttttgaa atcataccgc tgttagcgtg
     2041 cagatagcgg gttggcaggc acagcgcaac aaccggacgc ccgccgccca tcacgttgta
     2101 gcgcccgcca tccgtcgcac cggttttcat ggtggaaaat tgcagcggta aatcgttatg
     2161 tgcggcacag ctttttaacg ctgctaccag tttctggttg gggaagtagc gcttgtcaaa
     2221 cagcatcagc cccggcccct ggcccagttt cagcgggtat ttaatgttat caatgcccgg
     2281 aacatcgcct gcgacagcgg tatccagcac gatcactaca tccggtttaa tgtgttccgc
     2341 cgaggtttgt gccccgcgta gcccaacttc ttcttccacg ctgccgacgc catacagcgt
     2401 aatttcggga ttattcaccg tctgcaatag ttcagccatc attgcgcagc cgatgcggtt
     2461 atccagcgcc ttgccgacca ctttatcttc gccccagcag gcaaaattgg cttccgggct
     2521 aataaaatcg cccatcgcca caccgcgttt ttccacctca tcgcgactgt ttgcgccaat
     2581 atcaatgaac atctcatcaa atgacagcgg ccgttgcctt tgcttttccg ttaacgcatg
     2641 aggcgcgacg gaaccaatca caccagggat tttcactccc ttgtgtgtgc gtatggttac
     2701 ccggtggttg agcatcgact gattccacca gccaccaatg gtggtgaaac gcagaaaacc
     2761 gctctcgtcg atgtgggtga ccataaagcc gacttcatcc atatgcccga caacggcaac
     2821 ttttggacct ttattcccct tacgggcaac aaagctgccc agaccatcaa aggtgatctc
     2881 attaacgcaa ggttccagcg tgtttatcag aatgtcgcga acttcctgtt catcgccgct
     2941 gacggcgctg gcttcgcaca actgttgcag taactcaatg ttcatggaca agctcctgtt
     3001 gcgcggcttt cgctttgcgg cgtaaccaca atcctttcag gacgataatg aatgaaatgt
     3061 tcagcgcgag gccaatcgcc agcaccagat aaaacgaacc caccggcgac atcaggccga
     3121 tcagcgggtc aaagataccc aaaccaggtg ccagacgttt gattccgaaa gcgatcacca
     3181 gcacgccggt aatgccaccc gccagcgtat tggcggtaat catcggcaac ggagctgcca
     3241 gagcgtaagg tatagccggt tcggttgcga ctgtggcacc gaccacaatg gcactactgg
     3301 cagcctcttt ttcctgtcgg gtaaacagtt tgggcgcaat gaacgtcgca agacccgccg
     3361 caacgggcgg catcaacgcg acaacgccga cgatggcgta ccagtcataa atgtgttttt
     3421 ccagcagtga gaagcagaag aaccaggcgg ttttattgat ggggccgccc atatcgaacg
     3481 ccagcatcgc accgaccaga aacgctgctg caaacttcat tgatggagga atggtattca
     3541 ggaagtggag cagcccgccc atgaggtcgg acatcaccgg cccgataacg tagtaagtga
     3601 gcacgccgaa aaccagcaga gtaacgaacg ggatcagcat tgaaccgagc agcggttgta
     3661 atgctttgcc aaggcgcact ttgcggaacc agaagacgaa atagccaatc gccagcccca
     3721 gcaccaccgc gccgaggaat cccgcacccg actgggtgcc gagcagcgct ttgtcgttcg
     3781 ccagatagca aaccagaaac gccggagcga aagccggttt atcggcaatc gacgaggcaa
     3841 tataagcgcc catgatgggg atcataaagg tgaagccaag gtaaccgatg gattcaacta
     3901 cccaggtaaa cgacggcgcg cctttcgaca tatcggtata cggcaaaccg aactgcacca
     3961 gcatgttggc aagcgccacc aggataccac cgccgatgac aaacggcagc gccgcagaga
     4021 tgcccgccat caggtggctc atcacgctgc cgctttgcat ttcctgtttg ccaagcttca
     4081 cgccactatc tgcggcaaaa agctgcgagt ttgtcggtaa ttcgctgaaa atctggtcga
     4141 tatttttcaa cgcctgggag atggcaatct cataaacttt cttcccggta aaccgcgcgc
     4201 gatcatcacc gctcaggcca cgcccggtag cgagaatgac gtaatcggca gcggcaatct
     4261 cttcgctgga taagcggttt tcaacgccac tggacccttg agtttccact tttatggtat
     4321 ggccgagaga acgggctttc tgttccaggg cttccgccac catgtaggtg tgagcgatcc
     4381 cggcggggca gttggtgatc gcgacaatac gtaaggatga ctccatcgcc acttccttat
     4441 gagctgaggg tttgatttaa caaggcaagc acttggtcgg tatcgccctg ttgcagttga
     4501 tgaataaatt ccttgtgaat aattttgcga cacagtgtgc cgatgatttt gacctgatcc
     4561 tcttcgccgc tttgcggcac gccgaggcag atccagcaat tgacgtcttc gccatcgctg
     4621 gctttccagt caatagcctg cgctttgcgg gcgaataata cgaacggttg tttaacgcag
     4681 gcgcttttac cgtgcggcac ggcgacaccg gaaccaaatc cagtagagtg cattttttcg
     4741 cgcagcagca gggtttgcag gaactggtgt gagtcggtga taaaaccgtt ttgtaacgct
     4801 attgtcgcca actgcttcag aacggaataa gcgccattgc cctgaatatt caggtcaata
     4861 cagcttgcag taagagctgc cattaatcgg actccataaa atatatttta atcaattgta
     4921 atggtggagc gattaagtga tgagaggcga tatgtcggta aacaatgaca atttgagaca
     4981 gagtgaaagg tcagatttgc ggagtaatgc acataatggt tatttaaata aaccacatga
     5041 atcattaatg ggttattcat tatttttgtg atttactcac aatgtatcag ggaaatataa
     5101 cttaccggga gatgtaatat gtttaatttt tcaaatcgaa atttaaaata ttgtgccgga
     5161 ggcatctccg gcacattggg caattacggc aggtaaaaca cttcttgc
//
LOCUS       DADLFA010000219         5142 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000219 DADLFA010000000
VERSION     DADLFA010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5142
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.221"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(1..286)
                     /locus_tag="JQH65_003688"
                     /pseudo
     CDS             complement(1..286)
                     /locus_tag="JQH65_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Catalyzes the hydrolysis of AMP to form adenine and
                     ribose 5-phosphate using water as the nucleophile;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
     gene            complement(285..656)
                     /locus_tag="JQH65_003689"
                     /pseudo
     CDS             complement(285..656)
                     /locus_tag="JQH65_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            983..1058
                     /locus_tag="JQH65_003690"
     tRNA            983..1058
                     /locus_tag="JQH65_003690"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1016..1018,aa:Asn,seq:gtt)
     gene            complement(1114..2049)
                     /gene="ldtA"
                     /locus_tag="JQH65_003691"
     CDS             complement(1114..2049)
                     /gene="ldtA"
                     /locus_tag="JQH65_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAY6140956.1"
                     /translation="MMRRVNIFCSFALLFASHTSLAVTYPLPPEGSRLVGQSYTVTVP
                     DHNTQPLETFAAQNGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYEFDRKVPLPVTPSLR
                     AFISGQEVDVNRANAALQRRSGMPVPISSGSRQMF"
     gene            complement(2111..3190)
                     /gene="cobT"
                     /locus_tag="JQH65_003692"
     CDS             complement(2111..3190)
                     /gene="cobT"
                     /locus_tag="JQH65_003692"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HAY6140957.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGVPHVGKKAVLVMCADHGVWEEGGAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(3202..3945)
                     /gene="cobS"
                     /locus_tag="JQH65_003693"
     CDS             complement(3202..3945)
                     /gene="cobS"
                     /locus_tag="JQH65_003693"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HAY6140958.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILALLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(3942..4486)
                     /gene="cobU"
                     /locus_tag="JQH65_003694"
                     /pseudo
     CDS             complement(3942..4486)
                     /gene="cobU"
                     /locus_tag="JQH65_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
     gene            complement(4620..5057)
                     /locus_tag="JQH65_003695"
     CDS             complement(4620..5057)
                     /locus_tag="JQH65_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HAY6140959.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLADILPITCFVGEDWQQDFQ
                     PLDGGPVQSLSDSYSDYSVLLLIAAESEISVPEWLAHSEGTTVILIAEHNARLLTGPA
                     GGAVALIHYLNEICHSRETAVNDAFCRTLTRGSLAIPQCHLLS"
BASE COUNT         1351 a         1423 c         1141 g         1227 t
ORIGIN      
        1 taatctcgcc atgcaacggt ttatctgaaa cacacagtag tgtcccgtat ggcacgcgga
       61 aacgatatcc ttgcgcggca atggttgcac tttccatatc aattgctacg gcccggctta
      121 agttaaaacg aagtgcagaa gctgagtaac gtaattccca gttcctgtca tctgtggtta
      181 ccacagtacc agtacgtagc cgctgtttga cttcctcacc gggcctgccg ctcaccagct
      241 tggtggcgtc ataaagcgca cgttgcactt cagcaatgct cgggatagat aacgatctcc
      301 ggaaattcag cgcgaaccgc ctgtaataag ggcatggtga tggatgacgc agcggttcct
      361 ggtgcaaagc caatcgagac ttgccccgat aatgcctgac caacgttatg caccgccagt
      421 tgggcctgtt cacactgacg taaaatggcc cgcgcatggg tatagagaat ttttccggcg
      481 tctgttggcg taacgccccg ctttgtacgg atcaaaagtt gttgatttaa ctcaccttcc
      541 agtgtggcaa cctgctggct gagcgctggt tgtgcgatat gcaatacttc agcagcctgg
      601 gtcaggctac caatatctac aatttttacg aagtatttca ggcgtctgaa gttcatgttg
      661 cctccggttt ttaagaatcg gcccaagtgc cgccattact tacaaccaga ttgcaagatg
      721 cttgccagtt ttattttggc gttgatgtac aagctaacca actgtcaaat aagagattat
      781 gatagattcg tcatttgctc ctttaatcag ctgtcgcgtt cccctgccct ataaaaggag
      841 ggtatgcacc acgatggttc attacccaat aagattgaaa gctcaccact ttgttgaaat
      901 tgacagcaaa caaacaaaaa aatgcatttc accctttgac atcaccatgc actgccatta
      961 atatgcgccc cgttcacacg attcctctgt agttcagtcg gtagaacggc ggactgttaa
     1021 tccgtatgtc actggttcga gtccagtcag aggagccaaa ttcaaaaaag cctgctttct
     1081 agcaggcttt ttgctttcta attaccaacg ctcttaaaac atctgtcttg aaccagaact
     1141 aattggcaca ggcattcccg atcgacgttg caacgcagca tttgcgcgat ttacatcaac
     1201 ttcttgcccg ctgataaacg cccgcaaaga tggggttacc ggcaatggca cttttcggtc
     1261 aaactcatat tctgcacgat tgcgcgacaa cggctcatga acttccagcc agttcgagcc
     1321 atctggttca gtggtgtatt ttactggctg gtcgataatt tgcacacgcg tcccaacagg
     1381 aacattatca aacagatatt tgatatcgtc attgcgcaga cgaatacagc cctgacttac
     1441 ccggagccca ataccaaaat tggcattggt accatggatg gcatacaacc tgccaatata
     1501 aatcgcgtac agccccatgg gattatcggg gcccgcagga acaaatgcgg gcaaactctc
     1561 ccctcgtttc gcatattcgc gccgagtgtt cggcgttggc gtccaggttg gagcttcttg
     1621 tttacgttca acggtagtca cccagttacg cggggtttct cgcccagcct ggccgatacc
     1681 aataggaaag acttccacag tattactgtc tggtgggtag taataaagac gcatctcagc
     1741 gacgttaacc acaatccctt tacgaacagt ggcgggcaaa atcagttgct gcggaatggt
     1801 gagttgcgag ccggacttcg gcaagaaaac atcagcaccc gggttcgctt ccagcatgtt
     1861 acttaaccct tgcccgtttt gtgcggcaaa agtctccagc ggctgggtat tgtgatcagg
     1921 aacagttaca gtatacgact gccccactaa acggctaccc tctggaggta atgggtaagt
     1981 taccgccagg ctagtatggc tggcaaaaag cagagcaaat gagcaaaaaa tatttacacg
     2041 acgcatcatg tccctttcct aagtcgcgaa agctatccgt taagtatagc ttttatcaga
     2101 cttttcgttt ttaactgttc aaatcagaag tcgtattccc cggtagaaca atattactgg
     2161 cagcaagttc gcccatgttg ttatatatcg cacaggcagc ttcgatgatg ggcatcgcca
     2221 gagctgcgcc actcccctca cctaaacgca tctccatatt gagataaggc tccagcccca
     2281 aatgcgacag cgctatacgc gctccttttt ctgccgacag gtgagaagga atgagatacg
     2341 gtttgattgc aggagacatc tgacaggctg cgagcgcagc agcataagaa agaaatccat
     2401 ccagcaacac gggtaaaccg caggacgcag cgcctaacat cacgccagcc attccgacca
     2461 aatcaaatcc acccactttt gccaggacat caacgccatc ctgcggattt ggttgattca
     2521 acgtaatcgc ccggcgcaca acatcaattt tattagccag tttatctgtc ggcaggtttg
     2581 cgccaatccc aaccacttct tcaggagccc ggccagtgat tgtgctgact attgccgctg
     2641 ccggtgtcgt gtttgccatc cccagttcac ctacaccaaa cagcgtaaca ccgttttttg
     2701 ccagctcccg cgtataacat atgacgtcca aaagcaactt ttctgcctga tggcgactca
     2761 ttgccggagc tgaagcaata ttgccactac ctcgagcaac acgcatgttg ataagcccgg
     2821 ggataggctc agcagtatca ataccaacat caattacgta gacgttagcg cccgcttgtg
     2881 ctgccagcac acacacgccg gttgttccgc gggtcatatt ttcagcctga atggctgtca
     2941 cttcttttgg ggaaatagcg cccccttcct cccagacgcc gtgatcggca cacatgacca
     3001 gtaccgcttt tttgcccaca tgcggaacgc cattcaaccc cggcattcct gccagttgta
     3061 tggcaagcgc ctccagcctt cccaggctac caacaggttt gagtaacccg tcgatatgcc
     3121 gttgtgcacg cgacatagcg gcaggatcga tagcagggat cgtattcagt aaatcggcaa
     3181 gtgtttgaat gtcacgtctc tttatagcag tgccagcagg aagaccagtt caccaagctc
     3241 tatagcagct cccagcgtat cgccggtttg cccgcccagc gtacgtttga gaagctgacc
     3301 gaggatgaaa atcgccacca tcgtcaccac catagcagcc acaccatgca tacccggcaa
     3361 taatacggca gcaaaaatag cggctaagcc gagtgtgacg caggtttgtc gcccatcaat
     3421 tttgccaatg aacacattgc caagtccttc ttcccgggca taacgatgac gatacatcaa
     3481 taatgcggca gtcccgcggc tgaccgcaca tgctgccgcc agcaatgcaa ggatcggttc
     3541 gccacgcaga gccaactcgc ttaataccag aatctttgcc agtacaacaa aaatcaatgc
     3601 cagaccgccg tgggtgccta aacggctatc acgcatgatt tccaacatgc gatcgcggct
     3661 acgtgcagaa aatacgccat cacaggtatc ggcgaggccg tccaggtgga atcccccggt
     3721 catcagcgcc agcacaagta cgctaaacag tgccgccagt ggtacgccac accatgcctg
     3781 cagcaccatg aagaccagcc cgctaatcgc gccaagcaat aatccaatta aaggaaaagt
     3841 aataataccg cgagaataat gctcgaaatc cagtccctgg gaccagcgac gcggtacagg
     3901 cagacgcgta ataaaagaga gcatcgccca aaataattta ctcatttgat ttttactcca
     3961 atacccgaaa ccaccaacca tacttcattt gccgcagcgg ccagctgctg atttacccgc
     4021 ccggcaatat cacgaaaatg tcgtgccaga cgactctccg gcacaatccc cattcccact
     4081 tcgttagtca ctaatacaac ctttgcggga caacgttggc aggcagcaat caacgactga
     4141 atctcagcat taatcgcctg ttccatcgcc tgataatccc attcatcagg gtctttatcg
     4201 ccgccataat caaacaacag attagtcacc atggtggtaa cgcattccag caatacagcc
     4261 tcatgcgggt taatgtctgc atgaattaat tcatcaagat gttgccagcg ctccactgtg
     4321 tgccagtgcg tcgggcggcc ttgccgatga tgttctatcc gtgcagccat ctcatcatca
     4381 aggatttgcg aggtggcgta tacagaacct gtgaagagtc cccaataagc gcctctgcgt
     4441 ggcgactctt cccgctccgt acgccccccg tcaccgaaat catcataccg actcctgatt
     4501 caattgtatg atggtattca ttgaacagaa caatggatcc cccaccgttg ctgaccgcaa
     4561 aacatttacg cgattaatac tgcgcgtaat ataattaaaa atccagcatt ctcaatccat
     4621 tacgaaagaa gatggcattg cggaatagcc agacttcctc gtgttaacgt ccgacaaaat
     4681 gcgtcattga cagcggtttc tctggaatga cagatttcat tcagataatg gattaatgct
     4741 acagccccac cagccggacc agtcagcaaa cgggcattgt gttctgcaat caaaattacc
     4801 gtcgttccct cagaatgagc gagccattct ggtacggata tctcgctttc agctgctatc
     4861 aatagcagca ctgaataatc gctatacgaa tctgataacg attgcacagg cccaccatca
     4921 agaggctgga aatcttgctg ccagtcctca ccaacaaaac aggttattgg caatatatca
     4981 gccagatgtt tcgccagatc cacatctcca gataagaggc aaaaaagagc tatcgattcg
     5041 ggttttatcg ttttcatgcc tctcgccctc cacttaattt cctgtaatgg cgctctggtt
     5101 gataagccat gaaacaatat tgccacacct tgaatgcagc cc
//
LOCUS       DADLFA010000220         4962 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000220 DADLFA010000000
VERSION     DADLFA010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4962
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.222"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            389..1363
                     /gene="hemB"
                     /locus_tag="JQH65_003696"
     CDS             389..1363
                     /gene="hemB"
                     /locus_tag="JQH65_003696"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HAY6140960.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1469..2320)
                     /gene="tauD"
                     /locus_tag="JQH65_003697"
     CDS             complement(1469..2320)
                     /gene="tauD"
                     /locus_tag="JQH65_003697"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HAY6140961.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGITAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNCVTQHYANADYLPQRRIMHRVTILGD
                     KPFYRAG"
     gene            complement(2335..3144)
                     /gene="tauC"
                     /locus_tag="JQH65_003698"
     CDS             complement(2335..3144)
                     /gene="tauC"
                     /locus_tag="JQH65_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HAY6140962.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVSPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTP"
     gene            complement(3141..3908)
                     /gene="tauB"
                     /locus_tag="JQH65_003699"
     CDS             complement(3141..3908)
                     /gene="tauB"
                     /locus_tag="JQH65_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HAY6140963.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQVLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3921..4883)
                     /gene="tauA"
                     /locus_tag="JQH65_003700"
                     /pseudo
     CDS             complement(3921..4883)
                     /gene="tauA"
                     /locus_tag="JQH65_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
BASE COUNT         1181 a         1416 c         1245 g         1120 t
ORIGIN      
        1 tggacatata taatttatgg agaaaaacac gcatcaaatt gataaaagga cgatatttca
       61 acatgttgca gataatgtta tttttgatac atacgcgtta taaatatcaa aaacacaaac
      121 cagataaata tcttatcaat atacaacaaa gtttgaatta ttcaatttat agcagcacaa
      181 ccaaacgcaa aaaaaataat aaccaagagc tataaataaa gattaaaaat attttcagtg
      241 aatatgaact tattgctatt tctattacaa agatgtcctg gtctctcttt cattctcttc
      301 ttgtcaacat cacgacaact ttcgtaaaac actcctgccc cgcttcaggt atactgtgca
      361 ccttaattcc acaaacatca ggcagaccat gacagactta atccaacgcc ctcgtcgcct
      421 gcgcaaatct cctgcgctgc gcgctatgtt tgaagagaca acacttagcc ttaacgacct
      481 ggtgttgccg atctttgttg aagaagaaat tgacgactac aaagccgttg aagccatgcc
      541 aggcgtgatg cgcattccag agaaacatct ggcacgcgaa attgaacgca tcgccaacgc
      601 cggtattcgt tccgtgatga ctttcggcat ctctcaccat accgatgaaa ccggcagcga
      661 cgcctggcgg gaagatggac tggtggcgcg tatgtcgcgc atctgcaagc agactgtgcc
      721 agaaatgatc gtcatgtcag acacttgttt ctgtgaatac acttctcacg gtcactgcgg
      781 tgtgctgtgc gagcatggcg tcgacaacga cgcgactctg gaaaatttag gcaagcaagc
      841 cgtggttgca gctgctgcag gcgcagactt catcgcccct tccgccgcga tggacggcca
      901 ggtacaggcg attcgtcagg cgctggacgc tgcaggcttt aaagatacgg cgattatgtc
      961 gtattcgacc aagttcgcct cttcctttta tggcccgttc cgtgaagctg ccggaagcgc
     1021 attaaaaggc gaccgcaaaa gctatcagat gaaccctatg aaccgtcgcg aggcgattcg
     1081 tgagtcactg ctggatgaag cccagggcgc agactgcctg atggttaaac ctgctggagc
     1141 gtacctcgac atcgtgcgtg agctgcgtga acgtactgaa ttgccgattg gcgcgtatca
     1201 ggtgagcggt gagtacgcga tgattaagtt cgccgcgctg gcgggtgcta tagatgaaga
     1261 gaaagtcgtg ctcgaaagct taggttcaat taagcgtgcg ggtgcggatc tgattttcag
     1321 ctactttgcg ctggatttgg ctgagaagaa gattctgcgt taattttttt cctctcagaa
     1381 agggggagtt aacctgttga aagtacggtg aacgccccct caccctaacc ctctccccaa
     1441 gggggcgagg gaaccgtccg cactcgtatc accccgcccg ataaaacggt ttatccccaa
     1501 gaatcgtcac ccgatgcata atccgccgct gtggcaggta atcggcattg gcatagtgct
     1561 gagtcacgca gttatcccaa atcgcaatat cattcggttg ccagcgccaa cgcacctgaa
     1621 actccggttt agtgatatgg gcgaataaaa agcctaacaa ggcttcgctc tctttctcgc
     1681 tgacatcgac aattcgcgta gtaaagcctt cattcacaaa cagcgcctgt ttaccgctca
     1741 ccggatgcgt tcgcaccacc ggatgtagca acggaggatt tttcgcgacc gcctcgcgcc
     1801 agcgttgatg ttcctcctcg gttttgcggt atttgtattc cgggaacgat ttacggaaat
     1861 catgctccgc acgcagcccg ctcagcagct ggcggaaggg aacagagagc gcctcatagg
     1921 ccgtaatacc gctggtccag agcgtatcac cgccggtcga aggtaattct ttagctgcca
     1981 gaatcgcccc tgcgggtggc gtttcaataa atgtcacatc ggtatgccag ttgtcgttat
     2041 ctggcggatt atcgttatgg gtatccagca cgatgatctc gtcaacccct tcggcatgag
     2101 ggtaaacagg gtgaatatgc agttcgccaa aacgctgggc cagcgcgcgt tgctgctgcg
     2161 gcgtaatggc ttgatcgcgc agaaacacca cctgatggcg cagcaccgca tggtaaagct
     2221 gttcaaactg attatcgctt aacgggcgcg tcaggtcggc acccgaaatt tgtgcgccga
     2281 tatacggccc cagcggggta atgctcagac gttcactcat tgtacttctc catgctaggg
     2341 cgtcagacgg cgctgtaacg cgcgcaaacc cagttctaaa agaaaggcga taatcgcaat
     2401 caccgcgatt cccgccagca ccacgtcagt cgcgagaaat tcacccgctg actgaaccat
     2461 aaatcctaaa ccgcgcgtcg cggcaatcag ctccgccgct accagcgtag accagcccac
     2521 acccagacca atgcgtaatc cggtgaggat ttccggcagc gcgccgggca aaatgacaaa
     2581 ccacagcacc tgcgcacggc tggcacccag cgactgggcg gcacgaatgc gaacctgctg
     2641 cgcgcttttc acccccgcca gcgccgacat cgccaccggt gcaaaaatcg ctaaatagat
     2701 cagtaagatc ttcgaggttt caccaatacc aaaccagatc accatcagcg gcaaataagc
     2761 cagcggcgac accggacgat aaagttcgat taccggatcc agaatgccgc gcaccgtcgg
     2821 gcttagcccc atggcgatcc cgaccggaat accgatcacc accgccgcga gtagcgccag
     2881 cacaatgcgc gtcaggctgg ctgccagatg ctgccacagc gtggcgtcca taaatccctg
     2941 cggtccggca atggtgagca gttttgccag tacttgttgc ggcggcggca aaaacagcgg
     3001 gctaatcagt tgcagcgccg ccaccgtcca ccatacagtg agtaaaaccg ctaacgtgcc
     3061 aatgcttaag gtcacctgac gcgagagcgg ccagcgccat ttcagccgcc gcgaatgcag
     3121 tttttcatta atgagcacac tcatgagaac gcctcccgtt gctcaaatac gcggcttaaa
     3181 acatattcgc gcatggcgat gaattgtgga tcggacttga tgctgcggct cgactctccc
     3241 gcaacaaagc ggcgagcaaa gttgagcggc agccgctcca gcacacggcc agggccggat
     3301 gaaagcagaa ccagttcagt cgccataaac accgcttctt ctatatcgtg ggtaatcaac
     3361 agcacctgct tgcccgtctc ctgccagagt ttcagcagca gagtttgcat ctggtcgcgg
     3421 gtgaaggcgt ccagcgcacc aaacggttcg tcgagtaata acagctgggg attcgccgcc
     3481 agcgcgcggg caatccccac ccgctgacgt tgaccaccgg aaagctgcca gatatagcgt
     3541 ttttctgcgc cttccagccc cacttttttc agcacctggt gcgcgatttc cagtcgctgc
     3601 attttctcta tacctgccaa ttgcaggccg aacgccacgt tgtcctgtac attgcgccac
     3661 ggtagcagcc cttcattctg aaaaaccacc ccgcgctctg ctcccggtcc ctcaatacgc
     3721 ttacccgcca gttgaatgct gccatgctga taaggcacaa aaccggcaat cagattcagc
     3781 aacgtggttt taccgcagcc ggacggcccc agcaccacca gtagctcgcc gctttccagc
     3841 gtcaggttga tatcttccag tgccggtttg ccgccataat cggcatagag atgagagatt
     3901 tgcagcatct gcgcctcctt ttattgcacg aagcgcgagg taacgtactg gctgtaatca
     3961 ttcgctacag ccgggacctt gccctgctct ttcaaaaact gcgcggtgtc gatgattact
     4021 ttgttcaccg gtccggtcag ttctgccgtt tgttgctgcg gcgtcagata ggtattcccc
     4081 ttaaccagcc ccggaacgtc accttccggc acgccgctta aacgcgccag tttgctgatg
     4141 ttttccggct gtttcagcca cgcgtctggg ttagcaatgt acggttgctg agcatcgatg
     4201 gcgcttttag cgaacgcttt cacgacctca ggatgtttct cggcaaaatc tttgcgcacc
     4261 acccagacgt ccagcgttgg cgcaccccac tgcccgacct gttcagaatc ggtcaacacc
     4321 ttgccgtctt tttccagggc gttaaccgcc ggtgcccaga cataagcacc atcaatatca
     4381 ccccgctgcc atgcggcgat aatcgcgggc ggctgcaggt tcacaatctc cacttgccct
     4441 agtttaatcc cccagtgttt cagcgccgcc agcaggctgt agtgggtggt ggagataaac
     4501 ggtacggcaa tgcgtttgcc aatcaaatct tcgggtttgc tgatagtttt ctttaccacc
     4561 agcgcttcgg agttacccag ttttgacgcc agcaagaaga cttcaatcgg cacctgttgg
     4621 ctggctgcaa ccgctaatgg gctggaaccg aggttgccga tttgcacgtc gccagaagcc
     4681 agcgcccgca cgatgctggc tccgctgtca aacttacgtc agtccacgct cgccccgctc
     4741 tctttagcaa aggtgttgcc ggcctgagcc actttcgccg gttcggctga ggtttgatac
     4801 gccacggtga cgttcaccgc ctgtgcctga aaagcgatga atgccagtgc ggcaagaagt
     4861 gtgttacgcg atgaaattgc catgattgtc tgctccccag tcttgttatg ggagcagtat
     4921 tcaggaataa aaacattcat taaaagaatt agtcgttatc gc
//
LOCUS       DADLFA010000221         4960 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000221 DADLFA010000000
VERSION     DADLFA010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4960
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.223"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..247)
                     /locus_tag="JQH65_003701"
     CDS             complement(<1..247)
                     /locus_tag="JQH65_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3029 family protein"
                     /protein_id="HAY6140964.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLAN"
     gene            505..1284
                     /gene="deoC"
                     /locus_tag="JQH65_003702"
     CDS             505..1284
                     /gene="deoC"
                     /locus_tag="JQH65_003702"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAY6140965.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQTKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            1411..2733
                     /gene="deoA"
                     /locus_tag="JQH65_003703"
     CDS             1411..2733
                     /gene="deoA"
                     /locus_tag="JQH65_003703"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HAY6140966.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDARALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            2785..4008
                     /gene="deoB"
                     /locus_tag="JQH65_003704"
     CDS             2785..4008
                     /gene="deoB"
                     /locus_tag="JQH65_003704"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAY6140967.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            4088..4807
                     /gene="deoD"
                     /locus_tag="JQH65_003705"
     CDS             4088..4807
                     /gene="deoD"
                     /locus_tag="JQH65_003705"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAY6140968.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
BASE COUNT         1194 a         1263 c         1364 g         1139 t
ORIGIN      
        1 cgttcgccag aaaacgggcg taatcgggta agacatagcg cggtttgtac ggcgcatgac
       61 cttcaaacat atcgcagatt acaccctcat ccagcgcgcg gcgggcttcg gcaggaagct
      121 gcggataagg cagattgttt tctgcttcca gagccagaaa atggcgcttc tgctccgggc
      181 taagcactgg gctggtgaca atttgctggc aacgttgttg cagtgcattt tcatgagaag
      241 tgggcatctt cttttccttt tatgccgaag gtgatgcgcc attgtaagaa gtttcgtgat
      301 gttcactttg atcctgatgc gtttgccacc actgacgcat tcatttgaaa gtgaattatt
      361 tgaaccagat cgcattacag tgatgcaaac ttgtaagtag attaccttaa ttgtgatgta
      421 tatcgaagtg tgttgcggag tagatgttag aatactaaca aactcgcaag gtgaatttta
      481 ttggcgacaa gccaggagaa tgaaatgact gatctgaaag caagcagcct gcgtgcactg
      541 aaattgatgg acctgaccac cctgaatgac gacgacaccg acgagaaagt aattgctctg
      601 tgtcatcaga ccaaaactcc ggtcggcaat accgccgcta tctgtatcta tcctcgcttt
      661 atcccgattg ctcgcaaaac gctgaaagag cagggcaccc cggaaatccg aatcgctacg
      721 gtaaccaact tcccacacgg taacgatgac atcgaaatcg cgctggcaga aacccgtgcg
      781 gcaatcgcct acggtgctga tgaagttgac gttgtgttcc cgtaccgcgc gctgatggcg
      841 ggtaacgagc aggttggttt tgacctggtg aaagcctgta aagaggcttg cgcggcagcg
      901 aatgtactgc tgaaagtgat catcgaaacc ggcgaactga aagacgaagc gctgatccgt
      961 aaagcgtctg aaatctccat caaagcgggt gctgacttca tcaaaacctc taccggtaaa
     1021 gtggcagtga acgcgacgcc ggaaagcgcg cgcatcatga tggaagtgat ccgtgatatg
     1081 ggcgtagaaa aaaccgttgg tttcaaaccg gcgggcggcg tgcgtactgc ggaagatgcg
     1141 cagaaatatc tcgccatcgc agatgaactg ttcggtgctg actgggcaga tgcgcgtcac
     1201 taccgctttg gtgcttccag cctgctggca agcctgttga aagcgctggg ccacggtgac
     1261 ggtaagagcg ccagcagcta ctaagcaaga tgctttacgc ctgatgcgct gcgcttatca
     1321 ggcctacgag acgtatctac ccgtaggccg gataaggcgt agacgcatcc ggcaaaagcc
     1381 gcctcatact cttttcctcg ggaggttacc ttgtttctcg cacaagaaat tattcgtaaa
     1441 aaacgtgatg gtcatgcgct gagcgatgaa gaaattcgtt tctttatcaa cggcattcgc
     1501 gacaacacta tctccgaagg gcagattgcc gccctcgcga tgaccatttt cttccacgat
     1561 atgacaatgc ctgagcgtgt ctcgctgacc atggcgatgc gagattcagg aaccgttctc
     1621 gactggaaaa gcctgcatct gaatggcccg attgttgata aacattccac cggcggcgtc
     1681 ggcgatgtca cttcgctgat gttggggccg atggtcgcag cctgcggcgg ctatattccg
     1741 atgatctccg gtcgcggcct cggtcatact ggcggtacgc tcgacaaact ggaatccatc
     1801 cctggcttcg acattttccc ggatgacaac cgtttccgcg aaattattaa agacgtcggc
     1861 gtggcgatta tcggtcagac cagctcactg gctccggcgg ataaacgttt ctacgcgacc
     1921 cgtgatatta ccgcaaccgt ggactccatc ccgctgatca ctgcctcgat cctggcgaag
     1981 aaactggcgg aaggtctgga tgcgctggtg atggacgtga aagtgggtag cggcgcgttt
     2041 atgccgacct acgaactctc tgaagccctt gccgaagcga ttgttggcgt ggctaacggc
     2101 gctggcgtgc gcaccaccgc gctgctcacc gacatgaatc aggtactggc ctccagtgca
     2161 ggtaacgcgg ttgaagttcg tgaagcggtg cagttcctga cgggtgaata tcgtaacccg
     2221 cgtctgtttg atgtcacgat ggcgctgtgc gtggagatgc tgatctccgg caaactggcg
     2281 aaagatgacg ccgaagcgcg cgcgaaattg caggcggtgc tggacaacgg taaagcggca
     2341 gaagtctttg gtcgtatggt agcggcacaa aaaggcccga ccgacttcgt tgagaactac
     2401 gcgaagtatc tgccgacagc gatgctgacg aaagcagtct atgctgatac cgaaggtttt
     2461 gtcagtgaaa tggatgcccg cgcgctgggg atggcagtgg ttgcaatggg cggcggtcgt
     2521 cgtcaggcat ctgacaccat cgattacagc gtcggcttta ctgatatggc gcgtctgggc
     2581 gaccaagtag acggtcagcg tccgctggcg gttatccacg cgaaagacga aaacagctgg
     2641 caggaagcgg cgaaagcggt gaaagcggca attaaacttg ccgataaagc accggaaagc
     2701 acaccaactg tctatcgccg tatcagcgaa taacggtata ctgatctgat catttaaatt
     2761 tgaagcactg agtacggaga acatatgaaa cgtgcattta ttatggtgct ggactcattc
     2821 ggcatcggcg ctacagaaga tgcagaacgc tttggtgacg tcggggctga caccctgggt
     2881 catatcgcag aagcttgtgc caaaggcgaa gctgataacg gtcgtaaagg cccgctcaat
     2941 ctgccaaatc tgacccgtct ggggctggcg aaagcacacg aaggttctac cggtttcatt
     3001 ccggcgggaa tggacggcaa cgctgaagtt atcggcgcgt acgcatgggc gcacgaaatg
     3061 tcatccggta aagatacccc gtctggtcac tgggaaatcg ccggtgtccc ggttctgttt
     3121 gagtggggat acttctccga tcacgaaaac agcttcccgc aagagctgct ggataaactg
     3181 gtcgaacgcg ctaatctgcc gggttacctc ggtaactgcc actcttccgg tacggtcatt
     3241 ctggatcagc tgggcgaaga gcacatgaaa accggcaagc cgattttcta tacctccgct
     3301 gactccgtgt tccagattgc ctgccacgaa gaaacctttg gcctggataa actctacgaa
     3361 ctgtgcgaaa tcgcccgtga agagctgacc aacggcggct acaacatcgg tcgtgttatc
     3421 gctcgtccgt ttatcggtga caaagccggt aacttccagc gtaccggtaa ccgtcacgac
     3481 ctggctgttg agccgccagc accgaccgtg ctgcagaaac tggttgatga aaaacacggt
     3541 caggtggttt ctgtcggtaa aattgcagac atctacgcca actgcggtat caccaagaaa
     3601 gtgaaagcaa ctggcctgga cgcgctgttt gacgccacca tcaaagagat gaaagaagcg
     3661 ggtgataaca ccatcgtctt caccaacttc gttgacttcg actcttcctg gggccaccgt
     3721 cgcgacgtcg ccggttatgc tgcgggtctg gagctgttcg accgccgtct gccggagctg
     3781 atgtctttgc tgcgcgatga cgacatcctg atcctcactg ctgaccacgg ttgtgatccg
     3841 acctggaccg gtactgacca cacgcgtgaa cacattccgg tactggttta cggcccgaaa
     3901 gtaaaaccgg gttcactggg tcaccgtgaa accttcgcgg atatcggcca gactctggca
     3961 aaatattttg gtacttctga tatggaatat ggcaaagcca tgttctgata atgggtgcgg
     4021 cttgatgccc tcaccccggc cctctcccac agggagaggg tgaaaacatg ttgataagga
     4081 taaaacaatg gctaccccac acattaatgc agaaatgggc gatttcgctg acgtagtttt
     4141 gatgccaggc gacccgctgc gtgcgaagta tattgctgaa actttccttg aagatgcccg
     4201 tgaagtgaac aacgttcgcg gtatgctggg cttcaccggt acttacaaag gccgcaaaat
     4261 ttccgtaatg ggtcacggta tgggtatccc gtcctgctcc atctacacca aagaactgat
     4321 caccgatttc ggcgtgaaga aaattatccg cgtgggttcc tgtggcgcag ttctgccgca
     4381 cgtaaaactg cgcgacgtcg ttatcggtat gggtgcctgc accgattcca aagttaaccg
     4441 catccgtttt aaagatcatg actttgccgc tatcgctgac tttgacatgg tgcgtaacgc
     4501 ggtagacgcg gctaaagcac tgggcattga tgctcgcgtt ggtaacctgt tctccgctga
     4561 cctgttctac tctccggacg gcgaaatgtt cgacgtgatg gaaaaatacg gcatcctcgg
     4621 cgtggaaatg gaagcggctg gtatctacgg cgtcgctgca gaatttggcg cgaaagccct
     4681 gaccatctgt accgtgtctg accacatccg cactcacgag cagaccactg ccgctgagcg
     4741 tcagaccacc ttcaacgaca tgatcaaaat cgcactggaa tccgttctgc tgggcgataa
     4801 agagtaattg tgtttcgctg aaaggcgatt gtcgtgtgaa gccggagtgg gagactgctc
     4861 cggcttttta gtatctattc atcaatagca actgatttat agatttacta ttatttttca
     4921 tatggataga aaataaaatc tattcaagta tctattcacg
//
LOCUS       DADLFA010000222         4932 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000222 DADLFA010000000
VERSION     DADLFA010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4932
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.224"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(1167..1442)
                     /locus_tag="JQH65_003706"
     CDS             complement(1167..1442)
                     /locus_tag="JQH65_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6140969.1"
                     /translation="MTPEKHPETTMKRHRLTEAEERRLAEAIAAEARAMGLTKKDKMP
                     QETKRRLRARILKAILDERTASKNREATLTAGQDPDDTFTWQPLRRR"
     gene            1676..3769
                     /locus_tag="JQH65_003707"
     CDS             1676..3769
                     /locus_tag="JQH65_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-D"
                     /protein_id="HAY6140970.1"
                     /translation="MSDYEGSGPTEGIDYGHSMVVWPSTGLISGGDVKPGGSSGIAPS
                     MPPGWGDYSPQGIALVQSVLFPGIIRRIILDKELEEGDWSGWSVSVHSPWGNEKVSAA
                     RTVLENGLRGGLPEPSRPAAVSFARLEPASGNEQKIIRLMVTQQLEQVTDIPASQLPA
                     AGNNVPVKYRLMDLMQNGTQYIAIIGGIPMTVPVVDAVPVPDRSRPGTNIKDVYSAPV
                     SPNLPDLVLSVGQMNTPVLSNPEIQEEGVVAETGNYVEAGYTMSSNNHDVIVRFPEGS
                     GVSPLYISTVEILDSNGLSQRQEAENKAKDDFRVKKEEAVARAEAEKAKAELFSKAGV
                     NQPPVYTQEMMERANSVMNEQGALVLNNTASSVQLAMTGTGVWTAAGDIAGNISKFFS
                     NALEKVTIPEVSPLLMRISLGALWFHSEEAGAGSDIVPGRNLEAMFSLSAQMLAGQGV
                     VIEPGATSVNLPVRGQLINSNGQLALDLLKTGNESIPAAVPVLNAVRDTATGLDKITL
                     PAVVGAPSRTILVNPVPQPSVPTDTGNHQPVPVTPVHTGTEVKPVEMPVTTITPVSDV
                     GGLRDFIYWRPDAAGTGVEAVYVMLNDPLDSGRFSRKQLDKKYKHAGDFGISDTKKNR
                     ETLTKFRDAIEEHLSDKDTVEKGTYRREKGSKVYFNPNTMNVVIIKLNGEFLSGWKIN
                     PDADNGRIYLETGEL"
     gene            3766..4029
                     /locus_tag="JQH65_003708"
     CDS             3766..4029
                     /locus_tag="JQH65_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin D immunity protein"
                     /protein_id="HAY6140971.1"
                     /translation="MNKMAMIDLAKLFLASKITAIEFSERICVERRRLYGVKDLSPNI
                     LNCGEELFMAAERFEPDADRADYEIDDNGLKVEVRSILEKFKL"
     gene            4099..4245
                     /locus_tag="JQH65_003709"
     CDS             4099..4245
                     /locus_tag="JQH65_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072020761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HAY6140972.1"
                     /translation="MKKKIGGSMILVLAVLCLTACQANYVRDVQGGTIAPSSSSKLIG
                     VAVQ"
BASE COUNT         1240 a         1054 c         1290 g         1348 t
ORIGIN      
        1 atggagcact ttttatacaa tccgcactga tgccatatga ttaagccagt atacactccg
       61 ctatcgctac gtgactgggt cagggcttcg ccccgacacc cgctaaaacc cgctgacgcg
      121 ccctgacggg cttgtcggct cccggcatcc gctcacaggc aagctgtgac cgtctccggg
      181 agctgcctgt atcagcccgt tatgccatca tcgccgaagc gttcccggca acgttggtag
      241 cgttcatccg ccacttcgtg aagttgttct ggtgaaaaaa acacgtacag gacggggcag
      301 gatgtgcatt tgtcggaccg cgcacgcgca atcagacaaa tgccacctgg tcagggcaca
      361 gccccgacac cctggacaac accacagcaa taagataaaa cgtgcccggg cgcgtttctc
      421 tgcctctttt ttcatcatct ctgggattcg cgctcacccg tcctctgctg tcgggtttat
      481 gtatccccgt cgcccgtgga gatgaataac gggccggggg attaaaaatc cccgtgcagc
      541 acagccggcg ttgcataaaa ttccgctctg cgatgagtac tgtttaatca aaaatccccg
      601 cagttcctcc agaaaaacgc gagtctgttc tcatgccccg gatgcccaca ccccacgcaa
      661 aactaacttt ttgctgattt cttttttaaa tcagattgtt atgttttaaa aaatgtattt
      721 tcttattcag atttatacaa aagcgggtcg tcggcgacta ctggatgact cggcaacgag
      781 tcgtcggtga tttatcgatg actcaacaca ctgtcgctct ggtgttcaga gaaggcgagt
      841 cgccagagag tcgcgaggaa aaatactgga tgactcgccg acgactcccg tagtgaatcg
      901 ctcacgactc gtctgtaact acagtctgat ttcttcttat cagggctgaa aacgtcagaa
      961 aatacgcctt atgggcatca gacgcgccca aaccgctcgc cattcagttt tatgcatagt
     1021 catgcaacgc gctgaaagcg ttcctgacgc attttcccgg tttacccggg ggaaaacatc
     1081 catttttacg gtttttgcgc cagaagcgcg ctgagcgcgt tttaacggtg cgtacaatta
     1141 aggggttatg ttaaataacc cttagcttag cggcggcgca gtggttgcca ggtgaaggtg
     1201 tcatccgggt cctgtccggc agtcagtgtg gcttctcgat ttttgctggc cgttcgttca
     1261 tcaagaatgg ctttaagtat ccgtgcccgg aggcgtcgct ttgtttcctg cggcattttg
     1321 tcctttttcg tcagccccat ggcacgagct tcagcggcaa tggcttctgc cagtcggcgc
     1381 tcttcggctt cggtcagtct atggcgtttc atggtggtct ccgggtgttt ctctggtgtc
     1441 ataacagttt aactgttggc tctctcctta tgctaatcag ccttctttca tgcctgttgc
     1501 cattgagttt aagaatgatt ggttgttttt cttgagcgtt ttcacaaaaa cgtaaatcca
     1561 gtgggttgac aggatttacg aggtgacgta acttttcact gtacataaaa ccagtggttt
     1621 tatgtacagt ttgttgtttt taacgcattg ttttaaaagt caaagaggtg tttttatgag
     1681 tgattacgaa ggtagtggtc cgacagaagg tattgattat gggcactcga tggtcgtgtg
     1741 gccgtcaaca ggactgattt cgggtggtga tgtgaaacca ggaggctcat caggtatcgc
     1801 tccatccatg cctccgggat ggggggatta cagcccacag ggtatcgcac ttgtacaaag
     1861 tgttcttttt ccgggaatta ttcgccggat tattctggat aaggaacttg aagagggaga
     1921 ctggtcggga tggtctgtca gtgtgcatag cccttgggga aacgagaaag tttccgctgc
     1981 acgaacagtt cttgagaatg gtttacgtgg tggtttgcca gaaccgtctc gcccggctgc
     2041 tgtttctttt gcccgtctgg agcctgcttc cggaaatgag caaaaaatta ttcgtcttat
     2101 ggttacacag caactggaac aggtaacgga tattccggcc agccagttac cagcagcggg
     2161 taataatgta ccggtgaaat atcgtctgat ggaccttatg cagaacggta cgcaatatat
     2221 agctattatc ggaggtattc cgatgacagt gccggtagtt gatgccgttc cagttccgga
     2281 ccggagtcgt ccgggaacaa atattaaaga tgtttacagt gcccctgtat caccaaatct
     2341 accggacctg gtattaagtg tgggtcagat gaatactccg gttctgtcta atcccgaaat
     2401 tcaggaagaa ggagttgttg ctgagacagg taattatgtt gaggctggtt atacgatgtc
     2461 cagtaataat catgatgtca ttgttcgttt tcctgaaggc agtggtgttt ctccgttata
     2521 tatttcaact gtagagattc tggatagtaa tggtctgagt cagcgtcagg aagccgaaaa
     2581 taaagcaaag gatgatttca gagtcaagaa agaagaagcg gtagcccggg ctgaagctga
     2641 aaaagcaaaa gccgagttat ttagtaaagc aggtgtgaac cagcctcctg tatatacaca
     2701 ggaaatgatg gaaagggcta attcagtaat gaatgagcag ggagctcttg ttctgaataa
     2761 cactgccagt tctgtacaac tggcgatgac cggaactggt gtctggactg ctgctggtga
     2821 tattgcaggc aacataagca agtttttcag taatgctctg gagaaagtaa ccatacccga
     2881 agtgagtccc ctgcttatgc ggatttctct tggtgctctg tggtttcatt cagaagaagc
     2941 tggagcagga agtgatatcg tgccggggcg aaatctggag gcgatgtttt cgctgagtgc
     3001 tcagatgttg gctggacagg gcgtggtcat tgaacctggt gcgacgagtg taaatctgcc
     3061 tgttcgtgga caattgataa acagtaacgg gcaattagct ctggatttac tgaaaacagg
     3121 gaatgaaagt atcccggctg cggttcctgt tcttaatgct gtccgtgata cagcaacagg
     3181 actggataaa atcacgttac cagcagtagt aggcgcacct tcccggacga ttctggttaa
     3241 tccggtacca caaccttcgg tgccaacaga tacaggtaat catcaacccg ttccggttac
     3301 accagtgcat acaggaacgg aagtaaaacc ggtcgaaatg ccagtaacga cgattactcc
     3361 tgtttctgat gttggtggac tacgagattt tatttactgg cgtcctgatg ctgctgggac
     3421 tggtgttgaa gctgtttatg tgatgcttaa tgatcctctg gattcagggc gattttcgcg
     3481 taaacaactt gataaaaaat ataaacatgc tggtgatttt ggtattagtg atacaaaaaa
     3541 gaatcgtgaa actcttacta aattcaggga tgctattgag gagcatttat cggataagga
     3601 tacagtagag aaaggaacat accgaagaga aaaaggttca aaagtttatt ttaatcctaa
     3661 tacgatgaat gtggttataa ttaagttaaa tggtgagttc ttatctgggt ggaaaataaa
     3721 tccagatgcg gataatggtc gaatttattt agagacaggt gaactatgaa caagatggca
     3781 atgatcgatt tggcgaaatt atttttagct tcgaaaatta cagcaattga gttttcagag
     3841 cgaatttgtg ttgaacggag aagattgtat ggtgttaagg atttgtctcc gaatatatta
     3901 aattgtgggg aagagttgtt tatggctgct gagcgatttg agcctgatgc agatagggct
     3961 gattatgaaa ttgatgataa tggacttaag gttgaggtcc gatctatctt ggaaaaattt
     4021 aaattataat catgagtgat ttttatatct ggcatttaag tataaatgtc agatattcac
     4081 tcatgacaag gagatgtcat gaaaaaaaag atagggggat ctatgattct cgttcttgct
     4141 gttttgtgtc tgactgcttg ccaagctaac tatgtaaggg atgttcaggg aggaacaatt
     4201 gctccatcat catcttcgaa actaatcggg gtggcggttc agtgaaaaat attaaaggat
     4261 cttcttgaga tccttttttt ctgcgcgtaa tctgctgcct gtaaacgaaa aaacccgcct
     4321 tgtggcgggt ccttccgtcc gatcagaagt cttcgacctc ttctaatcgg cggtggtaac
     4381 tggcttggag gagcacagtc accaaaactg tcttttcagt ttagccttaa ctggttcata
     4441 acttcaagag caactcctct aaatcagtta ccaatggctg ctgccagtgg cgttaagtcg
     4501 tgtcttttcc gggttggccc caagagaaca gttaccggag aaggtgcagc agtcgggctg
     4561 aacggggggt tcctgcatac agcccagccc ggagcgaact gcctaagtcg ccccgagata
     4621 ccagaagacg tgagctatga gaaagcgcca cgtgtcccgc aagggagaaa ggcggaaaag
     4681 gtatccggca atcgatatgg cgggaacgtg ggggcgcagg agggagcgat tcaccggaaa
     4741 cggtgatgat ctttaagcca cgtcgggttt cgccaccgct ggcggatttc atggttgagt
     4801 cggaagactc ccacagatgc accgaaaaag tgtctgtttt gtgaagtccg tcaggggggc
     4861 ggagcctatg gaaaaagcgc ctccggcgcg gcctttttcg gcgcctcttt tttgctcaca
     4921 tgttttttcc gg
//
LOCUS       DADLFA010000223         4862 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000223 DADLFA010000000
VERSION     DADLFA010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4862
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.225"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            148..264
                     /locus_tag="JQH65_003710"
     CDS             148..264
                     /locus_tag="JQH65_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAY6140973.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            628..1470
                     /locus_tag="JQH65_003711"
     CDS             628..1470
                     /locus_tag="JQH65_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAY6140974.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            1473..2561
                     /gene="capU"
                     /locus_tag="JQH65_003712"
     CDS             1473..2561
                     /gene="capU"
                     /locus_tag="JQH65_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HAY6140975.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTW
                     KHPFRIIRQKTYLTQKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            2566..3516
                     /gene="virK"
                     /locus_tag="JQH65_003713"
     CDS             2566..3516
                     /gene="virK"
                     /locus_tag="JQH65_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HAY6140976.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLPCIEDILAIHPTLPARIHRPYLHKGGRAWTRGQYILEHYRFVQNL
                     PEKYSKFLFPQKSVSLVQFIGKDGENFDIQCSSSGFDREGELMLSLFYNKTVITRLTF
                     SVILTQNGHIAFIGGLQGAPKNTGPDVIRCATRACYGIFPKRIIFEAFCALMKACNIS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYRLPTQVIRKPLSD
                     IASKKRSEYRKRYALLDYIHETTIRSLDAYPVHSEHQDLN"
     gene            3581..4525
                     /gene="lpxM"
                     /locus_tag="JQH65_003714"
                     /gene_synonym="msbB"
     CDS             3581..4525
                     /gene="lpxM"
                     /locus_tag="JQH65_003714"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HAY6140977.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDP
                     VLAKIGRWAGRLSKKARRRATINLSLCFPEKSDTEREIIVDKMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRYNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
     gene            complement(4679..>4862)
                     /locus_tag="JQH65_003715"
     CDS             complement(4679..>4862)
                     /locus_tag="JQH65_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HAY6140978.1"
                     /translation="IYQPVYSPWVNHAERLWQALHDTITRNHQCRSMWQLLKKVRHFM
                     ETVSPFPGGKHGLAKV"
BASE COUNT         1386 a          922 c         1116 g         1438 t
ORIGIN      
        1 tatcggtggc aatagcctgt gcaaattttt tataacccgg gatcgtgtca ggattcagag
       61 tatgataaag acagattttc atggctgccg gtttccgtcg tgcaaaaaac atgctgattt
      121 tacgaaggaa tgccttactg aacaaccttg cccggaatac cccccgaaat cagatggaac
      181 aatcagaaaa atgctctgca gttatggaat aaagaaggtg gggtggtacg ccgggagggc
      241 gatccgctgg aaatggcgca gtagctgcag gcctgtcatg atgccggaat agaagttcgt
      301 gtacaggtta atgaaagcgt caccccgtaa ataaactctg cgctgaaatt cctttttttc
      361 attggagcac ctgtgttgtt ctgaggtgaa catatcacct ctgttcaggt agccaaattc
      421 agtgtgccac ctcaaggttc ttagtgagtc gtttttaaaa gatatgataa tatatcattg
      481 tttatgaatt gttgatgttt aatttagtaa aatatatcaa gtatgccatg ggtttgctat
      541 gttattcatc cagtatctgg aaataaaatg caccagcttt attgggtttt ccttttgtct
      601 ctgcttacta ggtgccagaa actttaaatg ttaaacgaag ggggcatttt gttcaaagcc
      661 aatcatgttc cggtattaat gtatcatcat gtcagtcatt gccccggact ggttacactt
      721 tctcccgtga cattccggaa gcaaatgaaa tggttggctg aaaataactg gaaaacactg
      781 tcatcagatg aactggagtt tttttatcgg ggcggaaaac tcccccggaa aagcgttatg
      841 ttgacatttg atgatggata ccttgacaac tggtttcagg tatatccttt attaaaagag
      901 tttaatctca aagcacatat ttttttaatt accggtttta ttgggaatgg tccggttcgt
      961 cactctcctg gaaaggaata ttctcatcgc gattgcgaac atcaaatagc gacagggaat
     1021 gctgataatg ttatgcttcg ctggtctgaa gtaaacgaaa tgttgcaatc tggtctggtc
     1081 gaatttcatg ttcatacgca tactcataca cgctgggata aaaagttcag ttcacgagaa
     1141 gaacagtgta aacatcttcg gcaggacttg ttgagtggga gagaatattt gaaagaaatg
     1201 acaggaaaat gcagtaaaca tttgtgttgg ccggaaggtt attataataa ggattatatt
     1261 caggtcgctg aagaacttgg attctattat ttatatacaa cagaaagaag aatgaatgct
     1321 cctgctaaag gaactacacg gataggaaga ataagcacca aagaacggga aagttgcgca
     1381 tggctgaaaa gacgactttt ttattataca acaccgtttt tttcatcgtt gctggccttt
     1441 cataaggggc caagattacc ggatgattaa taatgaatat actatttacg gaatcatcac
     1501 cgaatatagg tggtcaggaa ttacaggctg ttgctcaaat gaaggccctg aagaaaatgg
     1561 ggcattcagt tctgcttgtc tgcagggaaa acagcaaaat tgcttttgaa gccagtaaat
     1621 tgggaattga tatcacattc gcgttatttc gaaacagtct tcacatccct actgcatgga
     1681 gattactcgg aatagttcat ggttttcagc ccaatgcaat cgtttgtcac agtgggcatg
     1741 atagcaatat tgttggttta gtacggttat tcacttggaa acatccattc agaattatca
     1801 ggcaaaagac atatttgaca caaaaaacaa aagttttttc aataaatcat ttttgcgatg
     1861 aggtgattgt tcccggaaca agtatgaaga cacatctgga gcaggaagga tgtcgaaccc
     1921 gggttactgt tgtgcctcca ggctttgatt tccagaaatt atacgttgat tcccgaaaca
     1981 gtttgcctcc aaatgttctt tcttggctgg cgtcccgaag gggatgcccc gttattgctc
     2041 aggtaggaat gttgcgcccg gaaaaagggc acgaatttat gttgaattta ctgttccatt
     2101 taaaaatgaa tggacgacag ttctgttggt tgattgtggg gtctggttcg cctgaactgc
     2161 gggagcattt acagtatcag attgacagta tgggcatgca tgatgatgtt tttattgctg
     2221 acaatgtttt tcctgccgcc cccgtatatc gggttgccag tctggtggtt ctgccttcag
     2281 aaaacgaatc gtttggtatg gtactggcag aagcatcggc attttctgtg cctgtactgg
     2341 ccagtcagat tggtggaatc cctgatgtta ttcagaacaa ccagaccggg acattgttac
     2401 cagcaggtaa taagcacgca tggatgtgcg ccctgaatga tttttttaat gaccctgggc
     2461 gtttttatca gatggctcgc caggcaaaac aggatataga agagcggttt gatattaata
     2521 aaactgcgtt aaaaatactc acattagcga agcacaagta acaatatgtt ttctgtaagt
     2581 aacttatcat ttatcggttt ccttaaaagg attgtttttt cctcagattc actcccgggg
     2641 aagtgggaac acagaaaatt ccggttcatg tacattttgc gatgtgctat aaatccggtt
     2701 gccagtattc gatattatta cgaactgcgt tccttgccgt gcattgagga tattctggca
     2761 atacacccca cgttgccagc aagaattcat cgcccttatt tgcataaagg ggggcgcgcc
     2821 tggacccgtg gacaatatat tcttgagcat tatcgctttg tgcaaaattt gccagaaaaa
     2881 tattctaaat ttttgtttcc acaaaaatcg gtatcattag tccaattcat cgggaaagat
     2941 ggagagaatt ttgatatcca gtgttcttcc agtggttttg accgggaagg agagttgatg
     3001 ttgtcattgt tttacaataa aacagttatt acccggttaa cattttcagt cattctgact
     3061 caaaatggac acattgcttt tattggcgga ttacagggag cccctaaaaa taccggacct
     3121 gatgttattc ggtgcgcaac ccgggcctgt tatgggattt tcccaaaacg gattattttt
     3181 gaagcatttt gtgcgttgat gaaagcttgc aatatctctg agtgtctggc agtcagtgag
     3241 cacagtcatg ttttccggca attgagatac tggtatcaga aacgcaaaac ctttgttgcg
     3301 gtctatagtg atttctggga gtctgtagca ggaaaaacct gtggtgactg gtacagatta
     3361 ccaacccagg tgattcgaaa gccgctaagc gatatagcca gtaaaaaacg ctctgagtac
     3421 cgaaaacgat acgctttgct ggattatatc catgaaacta ctatccgctc tttggatgca
     3481 tatcctgtac actcagaaca tcaggattta aattaaggtt tgatgtattc tctgaataaa
     3541 acagtaatga taattttggt agaggcattc gcactaaata atgaaaaaat ataaatctga
     3601 gtttattcct gaatttaaga aaaattatct ttcccctgtt tactggtcta catggttcct
     3661 tttgggaatg attgcaggta tttcaatgtt tcccccttca ttcagagatc ctgtcttggc
     3721 aaaaataggg cgttgggcgg gcagattgag caaaaaagct cgtcgcaggg cgacgattaa
     3781 tttatcgctt tgtttcccag aaaagagtga tacagaacgg gaaataattg tcgacaaaat
     3841 gtttgccaca gcattgcaat ccatagtgat gatggcagaa ctggcaattc gtggtccgga
     3901 aaagttccag aaacgagtgt tctggaaagg cctcgaaatt cttgaggaaa ttcgatacaa
     3961 taacagaaat gtgatttttc tggttcccca tggctggagc gtggatattc ctgcaatgtt
     4021 gctggcagct cagggggaaa aaatggccgc catgtttcat cagcaacgaa atccagtgat
     4081 tgattatgtc tggaattctg tacggcgtaa attcgggggg cgcttacatt cccgggagga
     4141 tgggataaaa ccatttattc agtcagtacg ccagggatac tgggggtatt accttccaga
     4201 tcaggatcat ggtcctgaat acagtgaatt tgctgatttt tttgcgacct ataaagcgac
     4261 attaccaatt attggacgtc tgatgaacat cagtcaggct atgattatac cgcttttccc
     4321 ggtttatgat gaaaaaaaac atttcctgac tattgaagtt cggccaccaa tggatgcatg
     4381 cattgccagc gcggacaata aaatgattgc ccgacaaatg aacaaaacag tggaaatttt
     4441 ggtggggtca catccggaac agtatatctg ggttttaaaa ttgttaaaaa cgcgcaaatc
     4501 aaacgaagcg gacccgtacc cttgaaatta taaagtacag gtatttccac tagttgtttc
     4561 ttacaggtta ccaatcgaaa cacatccccc ttccgtgtgt tgcccgtttg gcacggagga
     4621 ctgtgtagat gcagttcgtt aaatttaatt tatgcctaaa tagctgcgct taataccgct
     4681 acacttttgc cagtccatgt ttgcctccgg ggaatgggct gacggtttcc ataaaatggc
     4741 gaactttttt caacagttgc cacattgagc ggcactgatg attacgcgtt attgtgtcgt
     4801 gaagtgcctg ccatagccgt tcagcatgat tcacccatgg cgagtaaacc ggctgataaa
     4861 tg
//
LOCUS       DADLFA010000224         4852 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000224 DADLFA010000000
VERSION     DADLFA010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4852
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.226"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..1250)
                     /locus_tag="JQH65_003716"
     CDS             complement(<1..1250)
                     /locus_tag="JQH65_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid carrier protein"
                     /protein_id="HAY6140979.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPRAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SMVICVINIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNDTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNTPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMA"
     gene            1529..2482
                     /gene="tal"
                     /locus_tag="JQH65_003717"
     CDS             1529..2482
                     /gene="tal"
                     /locus_tag="JQH65_003717"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HAY6140980.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPTLLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            2597..3184
                     /gene="mog"
                     /locus_tag="JQH65_003718"
     CDS             2597..3184
                     /gene="mog"
                     /locus_tag="JQH65_003718"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HAY6140981.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSVLTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(3219..3785)
                     /gene="satP"
                     /locus_tag="JQH65_003719"
     CDS             complement(3219..3785)
                     /gene="satP"
                     /locus_tag="JQH65_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HAY6140982.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YSGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(3934..4647)
                     /gene="msyB"
                     /locus_tag="JQH65_003720"
     CDS             complement(3934..4647)
                     /gene="msyB"
                     /locus_tag="JQH65_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HAY6140983.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAFDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            complement(4673..>4852)
                     /locus_tag="JQH65_003721"
     CDS             complement(4673..>4852)
                     /locus_tag="JQH65_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HAY6140984.1"
                     /translation="ARSASQSLNIPSEIKEGQTTDWININSDNDNKRCVSKITFSGHT
                     VNSSDMATLKIIGDD"
BASE COUNT         1275 a         1250 c         1229 g         1098 t
ORIGIN      
        1 gccattatga tatctgccag ttgccacatc agcggaaggc ttagcaaggt gccgccgatg
       61 accgttgcga aggtgcagat ccgcaaacac cagatcgctt taggggtgtt caggcgtaaa
      121 aagaagagat tgttttcggc ataaatgtag ttggcaacga tggagctgaa ggcaaacaga
      181 ataaccacga gggtaacaaa ctcagcaccc caggaaccca ttagcacccg catcgccttc
      241 tggataagct gaataccttc cagcggcatg taggttgtgt cgttacccgc cagtaatatc
      301 agcatggcgc ttgccgtaca gatgaccagg gtgtcgataa aaatgccaat catctggaca
      361 atcccttgcg ctgccggatg cggaggccag gacgccgctg ccgctgccgc gtttggcgtc
      421 gaacccattc ccgcctcatt ggaaaacata ctgcgctgaa aaccgttagt aatcgcctgg
      481 cttaaggtat atcccgccgc gccgcctgcc gcttcctgcc agccaaaagc actctcaaaa
      541 atagaccaaa tgacgtgggg aagttgcccg atatttatta cgcaaatcac catgctggtc
      601 agcacccaga ttatcgccat caacgggaca aacccctgca tgagccgggc gacgccatga
      661 agaccgcgag tgattgccag cagagcaaag acagcgagaa taatgcctgt caccagcggg
      721 ggaaaatcaa aagaaaaact cagggctcgg gcaacggcgt tcgcctgaac tccgctgaaa
      781 attatgccgt aggcgatgag caaaaagacg gcgaacagaa cgcccatcca acgcatcccc
      841 agcccgcgcg ccatatacca tgccggtccg ccacgaaact gcccattgac gtcacgttct
      901 ttataaagtt gtgcaaggga acattcggca aacgaggtcg ccatgccgat aaacgcggca
      961 acccacatcc aaaagacggc tctaggtcca ccggcggtaa tagccagcgc aacgccggcc
     1021 aggttgccgc tacccacgcg cgccgcaaga ctggtacaca atgactgaaa tgaggttaaa
     1081 ccgcctggct gtggatgaat gctattttta agacttttgc caaactggcg gatgtagcga
     1141 aactgcacaa atccggtgcg aaaagtgaac caacaacctg cgccgaagag caggtaaatc
     1201 attaccgatc cccaaaggac gctgttaata aaggagaaaa aatctggcat gcatatccct
     1261 cttattgccg gtcgcgatga ctttcctgtg taaacgttac caattgttta agaagtatat
     1321 acgctacgag gtacttgata acttctgcgt agcatacatg aggttttgta taaaaatggc
     1381 gggcgatatc aacgcagtgt cagaaatccg aaacagtctc gcctggcgat aaccgtcttg
     1441 tcggcggttg cgctgacgtt gcgtcgtgat atcatcaggg cagaccggtt acatccccct
     1501 aacaagctgt ttaaagagaa atactatcat gacggacaaa ttgacctccc ttcgtcagta
     1561 caccaccgta gtggccgaca ctggggacat cgcggcaatg aagctgtatc aaccgcagga
     1621 tgccacaacc aacccttctc tcattcttaa cgcagcgcag attccggaat accgtaagtt
     1681 gattgatgat gctgtcgcct gggcgaaaca gcagagcaac gatcgcgcgc agcagatcgt
     1741 ggacgcgacc gacaaactgg cagtaaatat tggtctggaa atcctgaaac tggttccggg
     1801 tcgtatctca actgaagttg atgcgcgtct ttcctatgac accgaagcgt caattgcgaa
     1861 agcaaaacgc ctgatcaaac tctacaacga tgctggtatt agcaacgatc gtattctgat
     1921 caaactggct tctacctggc agggtatccg tgctgcagaa cagctggaaa aagaaggcat
     1981 caactgtaac ctgaccctgc tgttctcctt cgctcaggct cgtgcttgtg cggaagcagg
     2041 cgtgttcctg atctcgccat ttgttggccg tattcttgac tggtacaaag cgaatactga
     2101 taagaaagag tacgctccgg cagaagatcc gggcgtggtt tctgtatctg aaatctacca
     2161 gtactacaaa gagcacggtt atgaaaccgt ggttatgggc gcaagcttcc gtaacatcgg
     2221 cgaaattctg gaactggcag gctgcgaccg tcttaccatc gcacctacac tgctgaaaga
     2281 gctggctgag agcgaagggg ctatcgaacg taaactgtct tacaccggcg aagtgaaagc
     2341 gcgtccggcg cgtatcactg agtccgagtt cctgtggcag cacaaccagg atccaatggc
     2401 agtagataaa ctggcggaag gtatccgtaa gtttgctatt gaccaggaaa aactggaaaa
     2461 aatgatcggc gatctgctgt aatcattctt agcgtgaccg ggaagtcggt cacgctacct
     2521 cttctgaagc ctgtctgtca ctcccttcgc agtgtatcat tctgtttaac gagactgttt
     2581 aaacggaaaa atcttgatga atactttacg tattggctta gtttccatct ctgatcgcgc
     2641 atccagcggc gtttatcagg ataaaggcat ccctgcgctg gaagaatggc tgacatcggt
     2701 gctaaccacg ccgtttgaac tggaaacccg cttaatcccc gatgagcagg cgatcatcga
     2761 gcaaacgttg tgtgagctag tggatgaaat gagttgccat ctggtgctca ccacgggcgg
     2821 aactggcccg gcgcgtcgtg acgtaacgcc cgatgcgacg ctggcagtag cggaccgcga
     2881 gatgcctggc tttggtgaac agatgcgcca gataagcctg cattttgtac caactgcgat
     2941 cctttcgcgt caggttggtg tgattcgcaa acaggcgctg atccttaact tacccggtca
     3001 gccgaagtct attaaagaga cgctggaagg tgtgaaggac gctgcgggta acgttgtggt
     3061 acacggtatt tttgccagcg taccgtactg cattcagttg ctggaagggc catacgttga
     3121 aacggcacca gaagtggttg ctgcattcag accgaagagt gcaagacgcg acgttagcga
     3181 ataaaaaaat ccccccgagc ggggggatct caaaacaatt agtgggattc accaatcggc
     3241 agaacggtgc gaccaaactg ctcgttcagt acttcaccca tcgccagata gattgcactg
     3301 gcaccacaga tcagaccaat ccagccggca aagtggatga ttgcggcgtt accggcaatg
     3361 ttaccgatcg ccagcagggc aaacagcaca gtcaggctaa agaaaacgaa ttgcagaacg
     3421 cgtgcgcctt tcagcgtgcc gaagaacata aacagcgtaa atacgcccca cagacccagg
     3481 tagacaccaa ggaactgtgc atttggcgca tcggtcagac ccagtttcgg catcagcaga
     3541 atcgcaacca gcgtcagcca gaaagaaccg taagaggtga atgcggttaa accgaaagtg
     3601 ttgccttttt tgtactccag cagaccagca aaaatttgcg cgatgccgct gtagaaaatg
     3661 cccatggcaa gaataatacc gtccagagcg aaataaccca cgttgtgcag gttaagcaga
     3721 atggtggtca tgccgaagcc catcaggccc agcggtgccg gattagccaa cttagtgttg
     3781 cccataattc ctcaaaaatc atcatcgaat gaatggtgaa ataatttccc tgaataactg
     3841 tagtgttttc agggcgcggc ataataatca gccagtgggg cagtgtctac gatcttttga
     3901 ggggaaaatg aaaattttcc ccggtttccg gtatcagacc tgagtggcgc taaccatccg
     3961 gcgcaggcag gcgatttgca gtacggctgg aatcgtcacg cgataggcgc tgccgctgac
     4021 cgctttaacc ccatttagtg ccgcacctac agggcctccc agccccgcgc cgcgcagcaa
     4081 accatgccca agtacgctca ttgctgcgtg ggtgcgtaaa atgcgggtca gttggctgga
     4141 aagcaaatgc gacacgcctt ttgccaataa tttgtctttc atcagcagcg gcagcagctc
     4201 ttccagctca ttcaccctcg catcgaacgc gtgcagaaac tcctgcttat gttcctcgtc
     4261 cattttcttc caggtattac gcagaaattg ttccagtaac tgttgctcaa tttcaaacgt
     4321 agacatctct ttgtcggctt tcagcttcaa tcgttttgaa acatcgagca aaatggcccg
     4381 atacaattta ccgtgtccgc gcagtttgtt ggcgatacta tcgccaccaa aatgctgtaa
     4441 ttctccagca atcagctgcc agttgcggcg atgttgctcg ggatgccctt ccatcgattt
     4501 aaacagttcg ttgcgcatca gtacgctgga gaggcgagtt ttgccttttt cattatgggt
     4561 gagcaatcgg gcgaaatttg ccaactgttc ctcactacaa tgctgaagaa aatccagatc
     4621 tgaatcattc aggtaattaa cattcatttt ttgtggcttc tatattctgg cgttagtcgt
     4681 cgccgataat tttcagcgtg gccatatccg atgagttcac cgtatgaccg gaaaaggtga
     4741 tttttgagac gcagcgttta ttgtcgttat cactgttaat gttgatccag tcagtggttt
     4801 gcccttcttt tatttctgaa ggaatattca gactctgact ggcgctacgg gc
//
LOCUS       DADLFA010000225         4835 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000225 DADLFA010000000
VERSION     DADLFA010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4835
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.227"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(64..511)
                     /locus_tag="JQH65_003722"
                     /pseudo
     CDS             complement(64..511)
                     /locus_tag="JQH65_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
     gene            668..1597
                     /gene="metA"
                     /locus_tag="JQH65_003723"
     CDS             668..1597
                     /gene="metA"
                     /locus_tag="JQH65_003723"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HAY6140985.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPEVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            1866..3467
                     /gene="aceB"
                     /locus_tag="JQH65_003724"
     CDS             1866..3467
                     /gene="aceB"
                     /locus_tag="JQH65_003724"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HAY6140986.1"
                     /translation="MTEQATTTDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASICDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEEHNNQVLNKVKADKSLEANNGHDGTWIAHSGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKMLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFGDAA
                     RLMEQITTSDELIDFLTLPGYCLLA"
     gene            3497..4794
                     /gene="aceA"
                     /locus_tag="JQH65_003725"
                     /pseudo
     CDS             3497..4794
                     /gene="aceA"
                     /locus_tag="JQH65_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
BASE COUNT         1213 a         1256 c         1226 g         1140 t
ORIGIN      
        1 aaaaaatgcc gggtgcgacg ctggcgcgtc ttctccagcc tgggcaggca cggcaggggc
       61 ggcttattcc cccacatacg ccagattcag caacggatac ggtttcccca aatcgtccac
      121 ctcagagcgt cccgtaacct taaaacccac cttcttatag aacccaaccg cctgctcatt
      181 ttgctcatta acgttggttg tcagttccgg tgccatcgag agcgcatgct ccaccagcat
      241 ccgacccacg ccgcagccgc acacatcagg atcgataaac agcgcatcca tatgctgccc
      301 acttaacaac ataaacccaa ccggctgatc ccgttcatta accgcgatcc acaacggcgc
      361 ttccggcagg aaggaacgaa ctagctagca cctccagctc ggcccgatac tctgctgata
      421 aaaaatcgtg tgtggcatcg acagatcgac accaaatcgc aacgagttcc tccccttcct
      481 catgccgtga gcggcgaata ctaataacca ttttctctcc ttttagtcat tcttatattc
      541 taacgtagtc ttttccttga aactttctca ccttcaacat gcaggctcga cattggcaaa
      601 ttttctggtt atcttcagct atctggatgt ctaaacgtat aagtgtatgt agtgaggtaa
      661 tcaggttatg ccgattcgtg tgccggacga gctacccgcc gtcaatttct tgcgtgaaga
      721 aaacgtcttt gtgatgacaa cttctcgtgc gtctggtcag gaaattcgtc cacttaaggt
      781 tctgatcctt aacctgatgc cgaagaagat tgaaactgaa aatcagtttc tgcgcctgct
      841 ttcaaactca cctttgcagg tcgatattca gctgttgcgc atcgattccc gtgaatcgcg
      901 caacacgccc gcagagcatc tgaacaactt ctactgtaac tttgaagata ttcaggaaca
      961 gaactttgac ggtctgattg taaccggtgc gccgctgggc ctggtggagt ttaatgatgt
     1021 cgcttactgg ccgcagatca aacaggtgct ggagtggtcg aaagatcacg tcacctcgac
     1081 gctgtttgtc tgctgggcgg tacaggccgc gctcaatatc ctctacggca ttcctaagca
     1141 aactcgcacc gacaaactct ctggcgttta cgagcatcat attctccatc ctcatgcgct
     1201 tctgacgcgt ggctttgatg attcattcct ggcaccacat tcgcgctatg ctgactttcc
     1261 ggcagcgttg attcgtgatt acaccgatct ggaaattctg gcagagacgg aagaagggga
     1321 tgcatatctg tttgccagca aagataagcg cattgccttt gtgacgggcc atcccgaata
     1381 tgatgcgcaa acgctggcgc aggaatattt ccgcgatgtg gaagccggac tagacccgga
     1441 agtaccgtat aactatttcc cgcacaatga tccgcaaaat acaccgcgag cgagctggcg
     1501 tagtcacggt aatttgctgt ttaccaactg gctcaactat tacgtctatc agatcacgcc
     1561 atacgatcta cggcacatga atccaacgct ggattaatct tctgtgatag tcgatcgtta
     1621 agcgattcag caccttacct caggcacctt cgggtgcctt ttttatttcc gaaacgcacc
     1681 tcaggaggtg aataaatttt attcacattg ttatcaataa gttatcaagc attttcaatt
     1741 aaaatggaaa ttgtttttga ttttgcattt taaatgagta gtcttagttg tgctgaacga
     1801 aaagcgcaca acgatccttc gttcacagtg gggaagtttt cggatccatg acgaggagct
     1861 gcacgatgac tgaacaggca acaacaaccg atgaactggc tttcacaagg ccgtatggcg
     1921 agcaggagaa gcaaattctt actgccgaag cggtagaatt tctgactgag ctggtgacgc
     1981 attttacgcc acaacgaaat aaacttctgg cagcgcgcat tcagcagcag caagatattg
     2041 ataacggaac gttgcctgat tttatttcgg aaacagcttc catttgcgat gctgattgga
     2101 aaattcgcgg gattcctgcg gacttagaag accgccgcgt agagataact ggcccggtag
     2161 agcgcaagat ggtgatcaac gcgctcaacg ccaatgtgaa agtctttatg gccgatttcg
     2221 aagattcact ggccccggac tggaacaaag tgatcgacgg gcaaattaac ctgcgtgatg
     2281 cagttaacgg taccatcagc tataccaatg aagcaggcaa aatttaccag ctcaagccca
     2341 atccagcggt tttgatttgt cgggtacgcg gtctgcactt gccggaaaaa catgtcacct
     2401 ggcgtggtga ggcaatcccc ggcagcctgt ttgattttgc gctctatttc ttccacaact
     2461 atcaggctct gttggcaaag ggcagtggtc cctatttcta tctgccgaaa acccagtcct
     2521 ggcaggaagc ggcctggtgg agcgaagtct tcagctatgc agaagatcgc tttaatctgc
     2581 cgcgcggcac catcaaggcg acgttgctga ttgaaacgct gcccgccgtg ttccagatgg
     2641 atgaaatcct tcacgcgctg cgtgaccata ttgttggtct gaactgcggt cgttgggatt
     2701 acatcttcag ctatatcaaa acgttgaaaa actatcccga tcgcgtcctg ccagacagac
     2761 aggcagtgac gatggataaa ccattcctga atgcttactc acgcctgttg attaaaacct
     2821 gccataaacg cggtgctttt gcgatgggcg gcatggcggc gtttattccg agcaaagatg
     2881 aagagcacaa taaccaggtg ctcaacaaag taaaagcgga taaatcgctg gaagccaata
     2941 acggtcacga tggcacatgg atcgctcact caggccttgc ggatacggca atggcggtat
     3001 tcaacgacat tctcggctcc cgtaaaaatc agcttgaagt gatgcgcgaa caagacgcgc
     3061 cgattactgc cgatcagcta ctggcacctt gtgacggtga acgcaccgaa gaaggtatgc
     3121 gcgcaaatat tcgcgtagcc gtgcagtaca tcgaagcgtg gatctctggc aacggctgtg
     3181 tgccgattta tggcctgatg gaagatgcgg cgacggctga aatttcccgt acctcaatct
     3241 ggcagtggat ccatcatcaa aaaatgttga gcaatggcaa accggtaact aaagccttgt
     3301 tccgccagat gctgggcgaa gagatgaaag tcattgccag cgaactgggc gaagaacgtt
     3361 tctcccaggg gcgttttggc gatgccgcac gcttgatgga acagatcacc acttccgatg
     3421 agttaattga tttcctgacc ctgccaggct actgcctgtt agcgtaaacc accacataac
     3481 tatggagcat ctgcacatga aaacccgtac acaacaaatt gaagaattac agaaagagtg
     3541 gactcaaccg cgttgggaag gcattactcg cccatacagc gcggaagatg tggtgaaatt
     3601 acgcgattca gtcaatcctg aatgcaccct ggcgcaactg ggcgcggcga gaatgtggcg
     3661 tctgctgcat ggcgagtcga aaaaagctac atcaacagcc tcggtgcact gactggcggt
     3721 caggcgctgc aacaggcgaa agcgggtatt gaagcagtct atctgtcggg atggcaggta
     3781 gcggcggacg ctaacctggc ggccagcatg tatccggatc agtcgctcta tccggcaaac
     3841 tcggtgccag ctgtggtgga gcggatcaac aatactttcc gccgtgcgga tcagatccaa
     3901 tggtccgcgg gcattgagcc gggcgatccg cgctatgtcg attacttcct gccgatcgtt
     3961 gccgatgcgg aagccggttt tggcggtgtc ctgaatgcct ttgaactgat gaaagcgatg
     4021 attgaagccg gtgcagcggc agttcacttc gaagatcagc tggcgtcagt gaagaaatgc
     4081 ggtcacatgg gcggcaaagt tttagtgcca actcaagaag ctattcagaa actggtcgcg
     4141 gcgcgtctgg cagctgacgt gacgggcgtt ccaaccctac tggttgctcg taccgatgcc
     4201 gcggatctga tcacctccga ttgcgacccg tatgacagcg aatttattac cggcgagcgt
     4261 accagtgaag gcttcttccg tactcatgcg ggcattgagc aagcgatcag ccgtggcctg
     4321 gcgtatgcgc catatgctga cctggtctgg tgtgaaacct ccacgccgga tctggaactg
     4381 gcgcgtcgct ttgcacaagc tatccacgcg aaatatccgg gcaaactgct ggcttataac
     4441 tgctcgccgt cgttcaactg gcagaaaaac ctcgacgaca aaactattgc cagcttccag
     4501 cagcagctgt cggatatggg ctacaaattc cagttcatca ccctggcagg tatccacagc
     4561 atgtggttca acatgtttga cctggcaaac gcctatgacc agggcgaggg tatgaagcac
     4621 tacgttgaga aagtgcagca gccggaattt gccgccgcga aagatggcta taccttcgta
     4681 tctcaccagc aggaagtggg tacaggttac ttcgataaag tgacgactat tattcagggc
     4741 ggcacgtctt cagtcaccgc gctgaccggc tccactgaag aatcgcagtt ctaagcaaca
     4801 acaaccgttg ctgactgtag gccggataag gcgtt
//
LOCUS       DADLFA010000226         4793 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000226 DADLFA010000000
VERSION     DADLFA010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4793
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.228"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..800
                     /locus_tag="JQH65_003726"
     CDS             <1..800
                     /locus_tag="JQH65_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HAY6140987.1"
                     /translation="SITNLNNQVTNLDTRVTNIENGIGDIVTTGSTKYFKTNTDGVDA
                     NAQGKDSVAIGSGSIAAADNSVALGTGSVAEEENTISVGSSTNQRRITNVAAGVNATD
                     AVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGSTTRISNVSAGVNNNDAVNYA
                     QLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAMTGLPQAYTPGASMASIG
                     GGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAALGAGIQW"
     gene            1168..2823
                     /gene="lldP"
                     /locus_tag="JQH65_003727"
     CDS             1168..2823
                     /gene="lldP"
                     /locus_tag="JQH65_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HAY6140988.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            2823..3599
                     /gene="lldR"
                     /locus_tag="JQH65_003728"
     CDS             2823..3599
                     /gene="lldR"
                     /locus_tag="JQH65_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HAY6140989.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            3596..4786
                     /gene="lldD"
                     /locus_tag="JQH65_003729"
     CDS             3596..4786
                     /gene="lldD"
                     /locus_tag="JQH65_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HAY6140990.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADTHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPAALAPMAK
                     GNAA"
BASE COUNT         1055 a         1271 c         1300 g         1167 t
ORIGIN      
        1 agagcattac taacctgaac aatcaggtca ctaatctgga tactcgcgtt actaatatcg
       61 aaaacggtat tggcgacatt gtcaccaccg gtagcaccaa gtacttcaag accaacaccg
      121 atggcgtaga tgccaacgcc cagggtaaag atagcgttgc tattggttct ggttccattg
      181 ctgccgctga caacagcgtc gcactgggta ccggttccgt tgcagaggaa gaaaatacaa
      241 tctctgtagg ttcttctact aaccaacgcc gtatcaccaa cgtagctgcc ggtgttaatg
      301 ccaccgatgc ggttaacgtg tcgcagctga aatcttctga agcaggcggc gtgcgctacg
      361 acaccaaagc tgatggttct atcgactata gcaatatcac cctcggtggt ggcaacggca
      421 gtacgactcg tatcagcaac gtctccgctg gcgtcaacaa caacgacgcg gtgaactacg
      481 cgcagttgaa gcaaagcgtg caggaaacga agcaatacac cgatcagcgg atggttgaga
      541 tggataacaa actgtctaaa actgaaagca agttgagcgg tggtatcgct tctgcaatgg
      601 caatgaccgg tctgccgcag gcttatacac cgggtgccag catggcttct attggtggcg
      661 gtacttacaa cggtgaatcg gcagttgctt taggtgtatc gatggtgagc gccaatggtc
      721 gttgggtcta caaattacaa ggtagtacca atagccaggg tgaatactcc gccgcactcg
      781 gtgccggtat tcagtggtaa tgatccatta agttagtgtg actaagggca aacgccaaat
      841 tgcctgatgc gttacgcgca tcaggcaaga taaggcgctc cgggtcaaca atctaagggc
      901 aattctctga tgaggattgc ccttttcttt accagacatc tccccccaca agaattggcc
      961 ctaccaattc ttcgcttatc tgacctctgg ttcacaattc cccaattaaa actcacatca
     1021 atattgccaa tacacaacat ttagttaacc attcattgtc attatcccta cacaacacaa
     1081 ttggcagtgc cacttttaca caacgtgtga caaggagatg agcaacagac tcattacacg
     1141 atgtgcgtgg actccaggag acctgcaatg aatctctggc aacaaaacta cgatcccgcc
     1201 gggaatatct ggctttccag tctgatagca tcgcttccca tcctgttttt cttctttgcg
     1261 ctgattaagc tcaaactgaa aggatacgtc gccgcctcat ggacggtagc aatcgccctt
     1321 gccgtggctt tgctgttcta taaaatgccg gtcgctaacg cgctggcctc ggtggtttat
     1381 ggcttcttct atggtttgtg gcccatcgcg tggatcatta ttgcagcagt gttcgtctat
     1441 aagatctcgg tgaaaaccgg gcagtttgac atcattcgct cgtctattct ttcgataacc
     1501 cctgaccagc gtctgcaaat gctgatcgtc ggtttctgtt tcggcgcttt ccttgaagga
     1561 gccgcaggct ttggcgcacc ggtagcaatt accgccgcat tgctggtcgg cctgggtttt
     1621 aaaccgctgt acgccgccgg actgtgcctg attgttaaca ccgcgccagt ggcatttggc
     1681 gcgatgggca ttccaatcct ggttgccgga caagtaacag gcatcgacag ttttgagatt
     1741 ggccagatgg tggggcgtca gctaccgttt atgaccatta tcgtgctgtt ctggatcatg
     1801 gcgattatgg acggctggcg cggtatcaaa gagacgtggc ctgcggtcgt ggttgcgggc
     1861 ggctcgtttg ccatcgctca gtaccttagc tccaacttca ttgggccgga gctgccggac
     1921 attatctctt cgctggtatc actgctctgc ctgacgctgt tcctcaaacg ctggcaacca
     1981 gttcgcgtat tccgcttcgg tgacttgggg gcgtcacagg ttgatatgac gctggcccac
     2041 accggttaca ccgcgggtca ggtgttacgt gcctggacac cgttcctgtt cctgaccgct
     2101 accgtaacac tgtggagcat cccgccgttt aaagccctgt tcgcatcggg tggtgcgctg
     2161 tatgagtggg tgatcaacat tccggtgccg tacctcgata aactggttgc ccgtatgcca
     2221 ccagtggtca gcgaggctac agcctatgcc gccgtgttta agtttgactg gttctctgcc
     2281 accggcaccg ccattctgtt tgctgcactg ctctcgattg tctggctgaa gatgaaaccg
     2341 tctgacgcta tcagcacctt cggcagcacg ctgaaagaac tggctctacc catctactcc
     2401 atcggcatgg tgctggcgtt cgcctttatc tcgaactatt ccggactgtc atcaacactg
     2461 gcactggcac tggcgcacac cggtcatgca ttcaccttct tctcgccgtt cctcggctgg
     2521 ctgggggtat tcctgaccgg gtcggatacc tcatctaacg ccctgttcgc cgcgctgcaa
     2581 gccaccgcag cacaacaaat tggcgtctct gatctgttgc tggttgccgc caataccacc
     2641 ggtggcgtca ccggtaagat gatctccccg caatctatcg ctatcgcctg tgcggcggta
     2701 ggcctggtgg gcaaagagtc tgatttgttc cgctttactg tcaaacacag cctgatcttc
     2761 acctgtatgg tgggcgtgat caccacgctt caggcttatg tcttaacgtg gatgattcct
     2821 taatgattgt tttacccaga cgcctgtcag acgaggttgc cgatcgtgtg cgggcgctga
     2881 ttgatgaaaa aaacctggaa gcgggcatga agttgcccgc tgagcgccaa ctggcgatgc
     2941 aactcggcgt atcacgtaat tcactacgcg aggcgctggc aaaactagtg agcgaaggcg
     3001 tgctgctcag tcgacgcggc ggcgggacgt ttattcgctg gcgtcatgac acatggtcgg
     3061 agcaaaacat cgtccagccg ctaaaaacgc tgatggccga tgatccggat tacagtttcg
     3121 atattctgga agcccgctac gccattgaag ccagcaccgc atggcatgcg gcaatgcgcg
     3181 ccacacctgg cgacaaagaa aagattcagc tttgctttga agcaacgcta agtgaagacc
     3241 cggatatcgc ctcacaagcg gacgttcgtt ttcatctggc gattgccgaa gcctcacata
     3301 acatcgtgct gctgcaaacc atgcgcggtt tcttcgatgt cctgcaatcc tcagtgaagc
     3361 atagccgtca gcggatgtat ctggtgccac cggttttttc acaactgacc gaacaacatc
     3421 aggctgtcat tgacgccatt tttgctggtg atgccgacgg ggcgcgtaaa gcaatgatgg
     3481 cgcaccttag ttttgttcac accaccatga aacgattcga tgaagatcag gctcgccacg
     3541 cacggattac ccgcctgccc ggtgagcata atgagcattc gagggagaaa aacgcatgat
     3601 tatttccgca gccagcgatt atcgcgccgc agcgcaacgc attctgccgc cgttcctgtt
     3661 ccactatatg gatggtggtg catattctga atacacgctg cgccgcaacg tggaagattt
     3721 gtcagaagtg gcactgcgcc agcgtattct gaaaaacatg tccgacttaa gcctggaaac
     3781 gacgctgttt aatgagaaat tgtcgatgcc ggtagcactg gcaccggtgg gtttgtgtgg
     3841 catgtatgcg cgtcgtggcg aagttcaggc agccaaagcg gcggacacgc atggcattcc
     3901 gtttactctc tcgacggttt ccgtttgccc gattgaagaa gtcgcgccag ccatcaagcg
     3961 cccaatgtgg ttccagcttt atgtactgcg cgatcgcggc tttatgcgta acgcgctgga
     4021 gcgagcaaaa gcagcgggtt gttcgacgct ggttttcacc gtggatatgc cgacaccggg
     4081 cgcacgctac cgtgatgcgc attcaggtat gagcggccca aatgcggcaa tgcgccgcta
     4141 cttgcaggcg gtgacgcatc cgcaatgggc gtgggatgtg ggcctgaacg gtcgtccaca
     4201 tgatttaggt aatatctcag cttatctcgg caaaccaacc ggactggaag attacatcgg
     4261 ctggctgggg aataacttcg atccgtccat ctcatggaaa gaccttgagt ggatccgcga
     4321 tttctgggat ggcccgatgg tgatcaaagg gatcctcgat ccggaagatg cgcgcgatgc
     4381 agtacgtttt ggtgctgatg gaattgtggt ttctaaccac ggtggccgcc agctggacgg
     4441 tgtactctct tccgctcgtg cgttgcccgc tattgcagat gcggtgaaag gtgatatcgc
     4501 cattctggcg gatagcggaa ttcgtaacgg gcttgatgtc gtgcgtatga ttgcgctcgg
     4561 tgccgacacc gtactgctgg gtcgtgcttt cctgtatgcg ctggcaacag cgggccaggc
     4621 gggtgtagct aacctgctaa atctgatcga aaaagagatg aaagtggcga tgacgctgac
     4681 tggcgcgaaa tcgatcagtg aaattacgca agattcgctg gtgcaagggc tgggtaaaga
     4741 gttgcctgcg gcactggctc ccatggcgaa agggaatgcg gcatagtcgt ttg
//
LOCUS       DADLFA010000227         4673 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000227 DADLFA010000000
VERSION     DADLFA010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4673
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.229"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            60..1859
                     /gene="cysJ"
                     /locus_tag="JQH65_003730"
     CDS             60..1859
                     /gene="cysJ"
                     /locus_tag="JQH65_003730"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HAY6140991.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKD
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDIEAADEFLSELRVERRYQRDVY"
     gene            1859..3571
                     /gene="cysI"
                     /locus_tag="JQH65_003731"
     CDS             1859..3571
                     /gene="cysI"
                     /locus_tag="JQH65_003731"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HAY6140992.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPHHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            3646..4380
                     /gene="cysH"
                     /locus_tag="JQH65_003732"
     CDS             3646..4380
                     /gene="cysH"
                     /locus_tag="JQH65_003732"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HAY6140993.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
BASE COUNT         1153 a         1218 c         1334 g          968 t
ORIGIN      
        1 tgttttatgg gcgctgacag ggcgcagaaa cagctttgct tactggaaca taacgacgca
       61 tgacgacaca ggtcccacct tccgcgttgc ttccgttgaa cccggagcaa ctggcacgcc
      121 ttcaggcggc cacgaccgat ttaacgccta cccagcttgc ctgggtttct ggctatttct
      181 ggggcgtact caatcagcag cctgctgcgc ttgcagcgac gccagcgcca gccgcagaaa
      241 tgccgggtat aactattatc tccgcctcgc aaaccggcaa tgcgcgccgg gttgctgaag
      301 cattacgcga tgatttatta gcagcaaaac tgaacgttaa gctggtgaac gcgggcgact
      361 ataaattcaa acaaatcgcc agcgaaaaac tgctcatcgt agtgacgtca acgcaagggg
      421 aaggggaacc gccggaagaa gccgtcgcgc tgcataagtt cctgttctcc aaaaaagcgc
      481 caaagctgga aaacaccgcg tttgccgtgt ttagcctcgg cgatagctct tatgaatttt
      541 tctgccagtc cgggaaagat ttcgacagca agctggcgga actgggtggt gaacgcctgc
      601 tcgaccgtgt cgatgccgat gttgaatacc aggctgctgc cagcgagtgg cgcgcccgcg
      661 tggttgatgc gcttaaatcg cgtgcgcctg tcgcggcacc ttcgcaatcc gtcgctactg
      721 gcgcggtaaa tgaaatccac accagcccgt acagcaaaga cgcgccgctg gtggctagcc
      781 tctctgttaa ccagaaaatt accgggcgta actctgaaaa agacgttcgc catatcgaaa
      841 ttgacttagg tgactcgggc ctgcgttacc agccgggtga cgcgctgggc gtctggtatc
      901 agaacgatcc ggcactggtg aaagaacttg tcgaactgct gtggctgaaa ggcgatgaac
      961 ctgtcaccgt cgagggcaaa acgttgcctc tgaacgaagc gctacagtgg cacttcgaac
     1021 tgaccgtcaa caccgccaac attgttgaga attacgccac gcttacccgc agcgaaacac
     1081 tgctgccgct ggtgggcgat aaagcgaagt tacagcatta cgccgcgacg acgccgattg
     1141 tcgacatggt gcgtttctct ccggcgcaac tggatgccga agcgctgatt aatctgctgc
     1201 gcccgctgac cccgcgcctg tattccatcg cctcctcgca ggcggaagtc gagaacgaag
     1261 tacacgtcac cgttggtgtg gtgcgttacg acgtggaagg ccgagcccgt gccggtggtg
     1321 cctccagctt cctcgcggat cgcgtggaag aagagggcga agtccgcgta tttatcgaac
     1381 ataacgataa ctttcgcctg cccgctaacc cggaaacccc ggtgattatg attggcccag
     1441 gcaccggtat tgcgccgttc cgcgccttta tgcagcaacg cgccgccgac gaagcgccag
     1501 gtaaaaactg gctgttcttt ggtaatccgc actttacgga agacttcctg taccaggtgg
     1561 agtggcagcg ctacgtcaaa gatggcgtgc tgacacgtat cgatcttgcc tggtcgcgcg
     1621 accaaaaaga aaaagtttac gtacaagaca aactgcgcga acagggcgcg gagctgtggc
     1681 gctggatcaa tgatggtgcc cacatttatg tctgcggcga cgctaatcgc atggcgaaag
     1741 acgttgagca ggcacttctg gaagtgattg ccgaatttgg tggcatggac atcgaagcgg
     1801 cggatgaatt tttaagtgag ctgcgcgtag agcgccgtta tcagcgagat gtctactaat
     1861 gagcgaaaaa catccagggc ctttagtggt cgaaggaaaa ctgacagacg ccgagcgcat
     1921 gaagcttgaa agcaactacc tgcgcggcac cattgcggaa gatttaaacg acggtctgac
     1981 cggcggcttt aagggcgaca acttcctgct gatccgcttc cacggcatgt atcagcagga
     2041 tgaccgcgac atccgcgccg aacgtgctga acagaagctg gagccgcacc acgcgatgct
     2101 gcttcgctgt cgtctgccgg gtggggtgat taccactaaa cagtggcagg cgatcgacaa
     2161 atttgccggt gaaaacacca tctatggcag cattcgcctg accaaccgcc agacattcca
     2221 gttccacggc attctgaaaa agaacgtcaa accggtgcac cagatgctgc actcggtcgg
     2281 tcttgatgcg ctggcgacag ctaacgacat gaaccgtaac gtactctgca cctcgaaccc
     2341 ttacgagtcg cagctgcacg cggaagcgta cgagtgggcg aagaagattt ctgagcatct
     2401 gttgcctcgt acccgcgcgt atgcggagat ctggctcgac caggaaaaag tcgccactac
     2461 tgatgaagaa ccgatccttg gtcagaccta cctgccgcgt aagttcaaaa ccacggtagt
     2521 gatcccgccg cagaacgata tcgatctgca tgccaacgac atgaacttcg tggcaatcgc
     2581 cgaaaacggc aagctggtag gcttcaacct gctggtgggc ggtgggcttt ctatcgaaca
     2641 cggcaacaag aaaacctacg cccgcacggc gagcgagttt ggctatctgc cgctggagca
     2701 tacgctggcg gtggccgaag ctgtcgtgac aactcagcgt gactggggta accgaaccga
     2761 tcgtaaaaat gccaaaacca aatacacgct ggagcgcgtg ggggttgaga cgtttaaagc
     2821 ggaagtggag cgtcgcgcgg ggatcaaatt tgaaccgatc cgtccatatg agttcaccgg
     2881 acgaggcgat cgtattggct gggttaaggg cattgatgat aactggcacc tgacgctgtt
     2941 tatcgaaaat ggtcgcatcc ttgattatcc ggggcgtccg ctgaaaaccg gcctgctgga
     3001 gatcgcgaag atccacaaag gcgatttccg cattacggcg aaccagaatc tgatcatcgc
     3061 cggtgtaccg gaaagcgaga aagcgaagat cgagaagatc gccaaagaga gcgggttaat
     3121 gaatgccgtc acgccgcagc gtgaaaactc gatggcctgc gtgtcattcc cgacttgccc
     3181 gctggcgatg gcggaagcag agcgtttcct gccgtctttt atcgacaaca tcgataattt
     3241 aatggcgaaa catggtgtca gcgatgagca tatcgtgatg cgtgtaacag gctgcccgaa
     3301 cggttgtggt cgcgcgatgc tggcggaagt gggcctggtg ggtaaagcgc cgggtcgcta
     3361 caacctgcat cttggcggca accgcattgg gacacgtatc ccacggatgt ataaagaaaa
     3421 catcaccgag ccggaaatcc tggcgtcgct tgatgaactg atagggcgct gggcgaaaga
     3481 gcgcgaagcg ggtgaaggct tcggcgactt tacggtgcgt gcgggcatca ttcgcccggt
     3541 gctcgatccg gcgcgcgatt tgtgggatta accatcagcc cggtcttgta ggcctgataa
     3601 gaacgcgtga gcgtcgcatc aggcaaggca aacagtgagg aatctatgtc caaactcgat
     3661 ctaaacgccc tgaacgaact gccgaaggta gatcgcattc tggcgctggc ggaaactaac
     3721 gccgaactgg aaaaactgga cgctgaaggc cgcgtagcct gggcgctgga taatctgccc
     3781 ggtgaatatg tactttcttc cagctttggc attcaggcgg cggtgagcct gcatctggtg
     3841 aatcaaattc gcccggatat tccggtgatc ctcaccgata cgggttactt gttcccggaa
     3901 acctaccgct ttattgacga gttaacggac aaactcaagc tcaacctgaa agtgtaccgt
     3961 gctaccgaaa gcgcagcctg gcaggaagca cgctacggaa aactgtggga gcagggcgtt
     4021 gaaggcattg aaaagtacaa tgacatcaac aaagtcgaac cgatgaaccg ggctctgaaa
     4081 gaacttaacg cgcaaacctg gtttgctggc ctgcgccgtg aacaatccgg cagtcgcgcc
     4141 aatttaccgg tgctggcaat tcagcgtggc gtatttaaag tgctgccgat tatcgactgg
     4201 gataaccgaa ctatttatca gtacctgcaa aaacatggcc tgaaatatca cccattatgg
     4261 gatgaaggat atttatcggt cggtgatacc catacaaccc gtaaatggga acctggcatg
     4321 tcggaagaag aaacacgttt ctttggctta aaaagggaat gtggattgca cgaagggtaa
     4381 atattgccgg ataaattaaa cgcctctgtc agaaatgatg ggggcgtttt tattttgcga
     4441 gcagtattcc tgattttaaa cgtgaacgcc gctggaagaa ttatcaggta tagaatggcg
     4501 tcgggtgctt gaggctgtct ggcgttaagc attcgcgagg ttccagatgg acaaaagccc
     4561 caggcgatat ttctatcaac ctgaggccag cgttcgaacc caaacaattc gaatgttagt
     4621 ctcttctttg cagggaggca agacatgctg acaaaatatg cccttgtggc aat
//
LOCUS       DADLFA010000228         4660 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000228 DADLFA010000000
VERSION     DADLFA010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4660
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.230"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..753)
                     /locus_tag="JQH65_003733"
     CDS             complement(<1..753)
                     /locus_tag="JQH65_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
                     /protein_id="HAY6140994.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLQQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLL"
     gene            complement(884..2248)
                     /locus_tag="JQH65_003734"
     CDS             complement(884..2248)
                     /locus_tag="JQH65_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine dehydratase 1"
                     /protein_id="HAY6140995.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(2432..3010)
                     /locus_tag="JQH65_003735"
     CDS             complement(2432..3010)
                     /locus_tag="JQH65_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HAY6140996.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVIGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            complement(3014..4375)
                     /gene="pabB"
                     /locus_tag="JQH65_003736"
     CDS             complement(3014..4375)
                     /gene="pabB"
                     /locus_tag="JQH65_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HAY6140997.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVAEPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVTKILRQ
                     LEK"
     gene            4449..4628
                     /locus_tag="JQH65_003737"
     CDS             4449..4628
                     /locus_tag="JQH65_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HAY6140998.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
BASE COUNT         1170 a         1197 c         1212 g         1081 t
ORIGIN      
        1 tagcagcacc gccagcggta attgtgttgg aagatagtgc catgccagcg ccgtagcccc
       61 cggaccatta acgttaatgg taaaaggaaa gcgttgcgaa gaaacctgat agttttcatt
      121 gtcttcaggt tgcggaaggg aatctaccag cccattacca tagagcaaat gccgtttgtc
      181 caccgtcagg ctggcgctac tgatttgctg cagttgtggt tcaaggagca ttgccgtcag
      241 taagtcgatg ttaatcatct ccatgacccc agcattgcta ctgcccgctg caggcgtcca
      301 ttgaatcaaa accggactgc ctttaatcag ggcacggtcg atcgtcaggc gtaaaagtgg
      361 ttgcggtgca ggaagttcag ccagaatgtc cacgacgggc acattgcgat aaccaaaaat
      421 gctggagcaa tagagtgtgc cgtcttgcac caggccaatg gatcgcacag tttggagttt
      481 tgccgcctgt ttacgtaatg gcaaatgggc gacagagcag ggcagaccaa tcagcggaag
      541 caagacttcg ctacctgcct gtaggggaag cagtacttta tctaattcct ctacagcgtg
      601 attggcgaat tgtactaccc gttgttgatt taagttacgc tgtgaaataa atcgcacgct
      661 cagggtcgaa gcgagcgtaa cgagtgcgca aatcgtacaa acaatcattc gattacgtcg
      721 ataggtttta atgatccgtt gtgctttttg catgcgggcc accctgtaag cgtatggcga
      781 caaacgcctg acgcaacagt ggatgtgtag tggggaatat tacagcagac gagaaagcgg
      841 gtataaattc gcccatccgt tgcagatggg cgagtaagaa gtattagtca cactggactt
      901 tgattgccag accaccgcgt gaggtttcgc ggtatttggc gttcatgtcc ttaccggttt
      961 cgtacatcgt ttcgatgacc ttatccagcg agacgcgcgg tgcactggtg cggcgcagag
     1021 ccatccgtgc ggcgttaatc gccttcacag aggcaatggc attacgctca atgcacggca
     1081 cctgaacctg ccctgcaacc gggtcgcagg ttaaaccaag gttgtgttcc atgccaattt
     1141 ccgccgccac gcaaacctgt tccgggctac cgcccagcag ttctgcaaga cccgcagcag
     1201 ccattgaaca ggcaacaccc acttcgccct ggcaaccaac ttccgcaccg gaaatagagg
     1261 cgttcatttt atacaatgca ccaatcgcgc ccgctgccat aaagtaacgg gtatagatgt
     1321 ccgggctgac cgattcaata aagtggtcat agtaagccag cactgccgga acgataccgc
     1381 aggcaccgtt ggttggcgca gttaccacac gaccaccggc ggcgttttct tcgttaactg
     1441 ccagcgcaaa catgtttacc cagtcaatga cattcatcgg atcgttagac agtttatcgc
     1501 tggaaaccag catccggcgc agggcagacg cacgacgtgg cacgcgcagc gggcctggca
     1561 gtacaccttc ggtgttcatc ccgcgatcga tacatgcctg catggtttgc cagacatgcg
     1621 cgaaatactc gtcgatctct ttcttgctgt gcagcgccag ttcgttctgc atagcgagac
     1681 cagacagcga atagccggtt tcattacagt acgcgagcag ttcggtggca gatttgaacg
     1741 gatacggcac gctcacttcg ttggcagcat cctgaccaaa gtgttcttca tcgacgataa
     1801 aaccgccgcc gatggaataa taagttttgc tgtagacgac ttcatcgccg ttataggcgt
     1861 ggatttgcat accgttttca tgcagcggca ggttgccgtt atgaaaacgc atcccgttgt
     1921 cgcgcgggaa atccacttca tgccgtccct gtgccagcag cagacgttcg cgctcttcta
     1981 cgtcgcgaat aaaaccggga atactgtcga tatccacggt ggcaggttcg ttacctgcaa
     2041 gacccataat aatggcgata tcggtgtggt ggcctttacc cgtcagcgac agtgaaccat
     2101 aaacgtccac ggcaacgcga gtaacgctat ccagtaagcc tttttcgacc agatcatcga
     2161 cgaactgttt acccgccttc ataggcccta cggtatggga agatgaggga ccaatcccca
     2221 ccttaaacat gtcgaataga ctaatcacga taatactcct gacaaggtga ctggacttcc
     2281 agtaacgaac taataacagc gcatagtgta agagggaacg cggcgactgg cttaactatt
     2341 cacatgaatt aaactaatca aaaaccgcga tttaactaat attgcgatga ttgtctcaag
     2401 tagtgttttg tagatgtaaa gataagtata gtcagggttt cacaccaatt tgcagcgcca
     2461 gctcacgaat tatgcctgcg gtcattcccc atacaaaata ctgttcgtac caggacagcc
     2521 atacccgatg tgaatcgcca cggcggtaga tatctaaagg gtgataacga cccagatgta
     2581 atgcctgggc gagcggcatt tcaaacaccg ccgagacttc atcttcactg gcgcgatacg
     2641 gcagatcggg cgggataatg ccgatcactg gggttacctg atagccagtt acgctatcga
     2701 cgggcggcag cacgccgata acttcaacgg cggaaggcgg tatagcgacc tcttcttcag
     2761 cttcgcgcag cgcggcggcg ataactgatg cgtccgtgtc atcgactgca cctccaggga
     2821 atgccacttg tccagcgtgt ttacgcagat gaattgaacg ctgagtcagc aacaaccccg
     2881 gttgcggtcg acggacgatg gggattaaca cggcagcctg acgatgattt agggtttccc
     2941 ggttaatttg cgggcgcaaa agttgaaagc gagataaaaa atcatcaagc gtcaggctac
     3001 ggtattccac gtcttacttc tccagttggc gtaatatctt agtaacttta tcaaaagttt
     3061 cctgatattc cgcttcttcc tggctatcgg cgacaattcc accgcccgca gagcagtata
     3121 tttgtccgtt aatggcagtc agcgtgcgga tagtaatgct ggtatccatg ttgccgcaaa
     3181 agctcaaata gccaatgctg ccgcaccagg cattacgtcg ctgcggttcc agttcgtcga
     3241 taatttccat agcccgtact ttcggagccc cggttattga gccaccagga aaagctgcgc
     3301 gcagcagatc gctggcgtgt aactgttctg gtagtcgcgc cgttatagtg ctgaccagat
     3361 gatgcacggc agggaagggt tccaccacga agagctctgg tacttttacc gaaccggcta
     3421 cggcaacacg accgatatca ttacgcatta aatcgacaat catcagattt tcggcacgat
     3481 ctttcgctga gttcgccagt ttttctgctt gtttgctatc ttcctgagga tcgggcaggc
     3541 gtggtagcgt gcctttaatc gggcgggtct ggatttcact attatcacaa agaataaacc
     3601 gctctggcga aaggcttaaa attgcaccct gttcaagacg taaaaaagcg ctaaatggcg
     3661 cgcggttggc ctgattaagc tgaaggaatg cctgccattc atcgccagaa taggtcgcat
     3721 gaaaacgctg ggcgagattc acctgatagc aatcaccgct gtgcagatat tcctgtacct
     3781 ggcgaaattt ttcgccgtac tgctcgcggg tcatattgga ttgccagtcg gaagtgagcg
     3841 tgaaatcttc ctgcggcgag aattgctggc tttccagcca ggcccgacgg gcattgacat
     3901 cattatgact cagcaaagaa actgtatgac gctggtggtc gacaatgagc gcccaatcgt
     3961 agatacccac tgccatatcc ggcagaacga tatcttgttc cgcaatttct ggcagtgact
     4021 caaaacggcg gcccagatcg tagccaaaca accccagtgc gccgccctga aatggcaaat
     4081 cttcgttatg cgttgggcga atgtctgcgc gatccagcac ctgctggagc acctgtagcg
     4141 ggtcatcagt ggtcgttgtg cgtttttcgc tttcactaac aacggtttct ttaccgaaag
     4201 tggttaaagt gcaaatcggc tcggcgacca caatatcaaa gcggctatac ggatgatcgg
     4261 catagccgga gtgtaaaagc atcgcccacg gcaggtggct taagcgggag aaataaaatt
     4321 cagcggcgtc ctgacgccag ggtaaagtaa tcacagcggg agataacgtc ttcatcagtc
     4381 ctgactctac tggctatgtg ccgggtagca tgaaataatt agcgccaaca atttagcagg
     4441 agttaacaat gtttgcaggt ttaccttcac tcacccatga acagcagcaa aaagctgtcg
     4501 agcggatcca ggaactgatg gcacaaggga tgagcagcgg ccaggcaatc gcgctggtgg
     4561 cagaagagct gcgtgccaac cactccggcg agcgaatcgt ggcgcgtttt gaggatgaag
     4621 acgagtaaca cactaatccg tatcgatttc gttatagaag
//
LOCUS       DADLFA010000229         4585 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000229 DADLFA010000000
VERSION     DADLFA010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4585
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.231"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            250..615
                     /gene="queD"
                     /locus_tag="JQH65_003738"
     CDS             250..615
                     /gene="queD"
                     /locus_tag="JQH65_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HAY6140999.1"
                     /translation="MMSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGE
                     VDPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPL
                     LSAVMVKETCTAGCIYRGE"
     gene            693..1961
                     /locus_tag="JQH65_003739"
     CDS             693..1961
                     /locus_tag="JQH65_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY6141000.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGVTTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWQLVGDALRSCVNTGISVRGMGMALTGAQAAQTLISACQHREP
                     QNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKPV
                     PPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            1952..2212
                     /locus_tag="JQH65_003740"
     CDS             1952..2212
                     /locus_tag="JQH65_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HAY6141001.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQWLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            2229..2804
                     /locus_tag="JQH65_003741"
     CDS             2229..2804
                     /locus_tag="JQH65_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HAY6141002.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDACNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(2952..3812)
                     /locus_tag="JQH65_003742"
     CDS             complement(2952..3812)
                     /locus_tag="JQH65_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HAY6141003.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGIQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(3809..>4585)
                     /locus_tag="JQH65_003743"
     CDS             complement(3809..>4585)
                     /locus_tag="JQH65_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HAY6141004.1"
                     /translation="NILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADEQ
                     AAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPEF
                     VARHIAEWQHQNPLDLIITGSQSSEGQNGQTPFLLAEMLGWTCFTQVERFTLDALFIT
                     LEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPAM
                     QCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
BASE COUNT         1053 a         1234 c         1166 g         1132 t
ORIGIN      
        1 agataaggtt aacggggtga acggtgtgga ttaaagacgg gatagcgata actaataacc
       61 aaatcgacta acctgtttta gcaatagtct ttaacaacaa aatagattaa ccaacccaat
      121 gaaaaacaaa tgaatttagc caatcattaa gataaatcag cgattttgcg caacaaagtc
      181 gttttagata atgcgaaaaa acagcctttc cggtactcta cggcggtttt atcgtcctgt
      241 agagaaatta tgatgtccac cacgttattt aaagatttca ccttcgaagc cgctcaccgc
      301 ttaccacacg tcccggaagg gcataaatgt ggtcgcctgc acgggcattc ctttatggtg
      361 cgactggaaa ttaccgggga agtcgatccg catacgggct ggattatcga tttcgctgaa
      421 ctaaaagcgg cgtttaagcc aacttatgag cgcctcgatc accattatct caatgatatt
      481 ccaggtctgg aaaacccaac tagcgaagtt ttagcaaaat ggatttggga tcaggttaaa
      541 cccgttgtgc cgctgttaag tgcggtgatg gtaaaagaaa cctgcaccgc tggttgtatc
      601 tatcgcggcg aatgataaga gtgtgtcggc ggtcaatttc ccttaagtaa cgctatgtta
      661 gggtattgtg ttcaggatat ctggggcatg acatggaaga cgactgcgac attattatta
      721 ttggtgccgg tattgcaggc accgcttgcg cgttacgctg cgcgcgagcg ggtttatccg
      781 ttttgttact ggaacgcgct gaaatccccg gcagcaaaaa tctttccggc ggacggttat
      841 atacccatgc actcgcggaa ctcctccctc agtttcatct gaccgcgcct cttgaacgac
      901 gcatcactca cgaaagcctt tccctgttaa cgcccgatgg cgtaacgacg ttttccagct
      961 tacagcccgg cggtgaatcc tggagtgtat tacgtgcacg gttcgatccg tggctggttg
     1021 ccgaagccga aaaagaaggt gtcgaatgta tccccggagc gacggtggat gcactgtatg
     1081 aagaaaacgg cagagtctgt ggcgttattt gtggtgacga tattctccgc gcccgttatg
     1141 tggtgctggc agaaggtgcc aacagcgtcc tggctgaacg tcacgggtta gtgactcgtc
     1201 ctgctggcga agcgatggcg ttggggatca aagaagtgct gtcgctggaa acatccgcta
     1261 ttgaagaacg ttttcatctg gagaataacg aaggcgcagc gttgctgttc agcggcggaa
     1321 tctgtgatga cttacccggc ggcgcatttc tttatactaa tcaacaaacg ctctcgttag
     1381 ggattgtttg cccgctctct tcccttacgc aaagtcgtgt tccggcaagc gagctgctga
     1441 ctcgctttaa agcgcatccg gcagtgcgcc cgcttatcaa aaacacggaa tcactggagt
     1501 atggtgcgca tctggtgcca gaaggtggct tgcacagtat gccggtgcaa tacgccggta
     1561 acggctggca gctggtgggc gatgcgttgc gcagttgcgt caataccgga atttccgtgc
     1621 gcggcatggg tatggcgctg actggcgcgc aggcggcaca aacgctgata agcgcctgcc
     1681 agcaccgcga gccgcaaaat ctgtttgcgc tttatcatca caacgtagag cgcagcctgc
     1741 tatgggatgt tctacaacgt tatcagcatg ttccggcgct tttgcaacgc cctggctggt
     1801 atcgggcgtg gcctgcgtta atgcaggata tttcccgcga tttatgggat cagggtgata
     1861 aacctgttcc accgctgcgc cagttattct ggcgtcattt acgtcgtcat ggcctgtggc
     1921 atctggcggg cgatgttatc aggagtctgc gatgtctgta gcccataatc tctggcgcgt
     1981 tgctgatgcg ccgcacattg ttccggctga ctccgttgag cgtcagatgg tacaatggtt
     2041 gattaacgcc tgtccggcag gtcttttttc gctcacaccg gaaggtgact tacgcgttga
     2101 ttatcacggc tgcctggagt gtggcacctg ccgtttgctg tgcgacgaat ccacactaca
     2161 acagtggcgc tatccgcctt ccggattcgg catcacctac cgctttggat aaataataag
     2221 gataatttat gcccctctta cacctgctcc gccagaatcc ggtgattgct gccgttaaag
     2281 ataatgccag cctgcaactg gcaatcgact ctgaatgcca gtttatttcc gtgttgtacg
     2341 gcaatatctg caccatcagt aatatcgtta aaaagattaa gaacgccggg aaatatgctt
     2401 ttattcatgt tgatttgctg gaaggcgcgt caaataaaga agtcgtgatt cagtttttga
     2461 aactggtgac cgaggcggac ggcattatca gtaccaaagc ctcaatgttg aaagcggcaa
     2521 gagcggaagg ttttttctgt attcatcgcc tgtttattgt tgattcaatt tcgtttcaca
     2581 acattgataa gcaagttgcg caatcgaatc cggattgtat tgagatcctg ccaggctgta
     2641 tgcccaaagt gctgggctgg gtgacagaga aaatccgcca accgctgatt gccggtgggc
     2701 tggtgtgcga tgaagaagat gcgtgtaatg cgattaacgc gggtgtcgtg gcgctttcca
     2761 ccacgaatac cggggtctgg acgttagcga aaaaattact ttgacgggat aatcgtaacc
     2821 aattgaattt ggtttgattt gtaggccgca cgccacatcc gacattcagc gcctgatgcg
     2881 acgcttgacg cgtcttatca ggcctacaag tccccaggcc agaaccgtag gcaggatcat
     2941 ccggcaacac attattggca atctgcgtga atgtttgtca ccagtgcctc cagcaccact
     3001 ttccagtcat caaccacgcc aacatctgcc tgtgaaaata ccgcagcgct ggcgtcatga
     3061 ttaatcgcca ccacaaactt gctattgcgt acgcccgcca ttaacgctgc tgcgccggaa
     3121 gcgccaacaa caatacacac ctcaggtgcc agcaaatgtc cggaaatacc aatcactttt
     3181 tcagcatcaa cgccaccgtt catcactcgc gcccgactgt agcccacttc ggctcccagc
     3241 ttttctgcca gcatagcgat ttcctggtta tctgcctctc cgccctgccc caccaccagc
     3301 acccgtctgg cctcagctaa tgggtcgcga gtaacatttt tcagatcctc tgtgctaacc
     3361 agccagtcgg gaagcgcacc cgggacaatg ttcagttgtt gtattccaga gggcaacgtg
     3421 gcgtttttcg ctgctcccgc ctgacgcgcc agagaaaggc atagcgggcg tttttctgtt
     3481 tgcagcgtcg ccgttagcgc atttccccag tgggatttgc gcacgcttac ggttggaata
     3541 tctaacgaag tcacctggca gatgcttgca ccatgtaaac gccaggccag ccgggtcgac
     3601 agttcatcgc caaacgttcc cggcggaaag agaacaacgt ccgcaggcgt tcgctgccac
     3661 tgttccacca acgcatccag cacctgttcc gccactacag gctgaggttc aatctgccag
     3721 tgcgccaggg tacaaccact gaaatcctgc gccgccagcc agcttgctat tgcggcattc
     3781 tcctgattta ttgtcacaat tgcgatattc atggctgcat cctttggcgt aaatagtcct
     3841 gccatagctt ttgagctttt tctgctaccg tttgcccatc aatcagcgtc gctccgcgtc
     3901 gttgctctgc tcttgccagt tgcagacact gcatcgctgg catttcggca gcaacagttt
     3961 tacggataat ttctgctttc cccgcagcca ttcgctggcg cattcccggt actggcaagg
     4021 ccacttcacc gcactgacgc acggcaatca ccgcaggcaa gcgaacccgg caacaacgca
     4081 acccatgttc agtacgctgt tcgagggtga taaacagcgc gtcgagagtg aaacgctcca
     4141 cctgggtgaa gcaggtccag cccagcattt ccgccagcaa aaatggcgtt tgcccattct
     4201 gcccttcgct gctttggctg ccggtaatta tcagatccag cggattctga tgctgccatt
     4261 cggcaatatg gcgagcgaca aattccggcg caaagcgcag atctgccgcc gtctccagca
     4321 atacagcttc ctcaaaccca agagccatga gatagcgcag ccagtgcaat gcccgttcat
     4381 cccccatgct caacgcggtt aaagacatcg gcgtaccgtt ttttctctgc gccaacagca
     4441 gcgcggcagc ggcctgttca tcagcaccga gtaaacttcg cagtagcgaa atatccggtc
     4501 cgcttttacc ctgagccgcc gcctgccact ctttttccgc cagcattccg gcatccggtt
     4561 cggctttaaa cgctaacaga atgtt
//
LOCUS       DADLFA010000230         4562 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000230 DADLFA010000000
VERSION     DADLFA010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4562
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.232"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            92..850
                     /locus_tag="JQH65_003744"
     CDS             92..850
                     /locus_tag="JQH65_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012599877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAY6141005.1"
                     /translation="MSFMSSFLLGRFLHPGVFSLCVLLPLLASATTSHISFSYAARQR
                     MQNRARLLKQYQTHLKKQASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLKASDRR
                     WQALVENNHFLSSNHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNK
                     ILEAKLPRTANEMYHYHRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIES
                     PRTGETIRVSRLAEPFWQDHFLGARWILTEETIL"
     gene            1026..1523
                     /gene="rayT"
                     /locus_tag="JQH65_003745"
     CDS             1026..1523
                     /gene="rayT"
                     /locus_tag="JQH65_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HAY6141006.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTTQYQMLRNAIIKVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDVTDINAG
                     ERIIL"
     gene            complement(1747..3486)
                     /gene="flhA"
                     /locus_tag="JQH65_003746"
     CDS             complement(1747..3486)
                     /gene="flhA"
                     /locus_tag="JQH65_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhA"
                     /protein_id="HAY6141007.1"
                     /translation="MLSRSDLLTLLTINFIVVTKGAERISEVSARFTLDAMPGKQMAI
                     DADLNAGLINQAQAQTRRKDVASEADFYGAMDGASKFVRGDAIAGMMILAINLIGGVC
                     IGIFKYNLSADAAFQQYVLMTIGDGLVAQIPSLLLSTAAAIILTRVSDNGDIAHDVRN
                     QLLASPSVLYTATGIMFVLAVVPGMPHLPFLLFSALLGFTGWRMSKRPQAAEAEEKSL
                     ETLTRTITETSEQQVSWETIPLIEPISLSLGYKLVALVDKAQGNPLTQRIRGVRQVIS
                     DGNGVLLPEIRIRENFRLKPSQYAIFINGIKADEADIPADKLMAQPSSETYGEIDGVL
                     GNDPAYGMPVTWIQPAQKAKVLNMGYQVIDSASVIATHVNKIVRSYIPDLFNYDDITQ
                     LHNRLSSMAPRLAEDLSAVLNYSQLLKVYRALLTEGVSLRDIVTIATVLVASSAVTKD
                     HILLAADVRLALRRSITHPFVRKQELTVYTLNNELENLLTNLVNQAQQGGKVMLDSVP
                     VDPNMLNQFQSTMPQVKEQMKAAGKDPVLLVPPQLRPLLARYARLFAPGLHVLSYNEV
                     PDELELKIMGALM"
     gene            3431..4216
                     /gene="lafU"
                     /locus_tag="JQH65_003747"
     CDS             3431..4216
                     /gene="lafU"
                     /locus_tag="JQH65_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HAY6141008.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSITTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHIDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
BASE COUNT         1088 a         1202 c         1193 g         1079 t
ORIGIN      
        1 tgaaacctca atagtaatat gcaaataagc aattaataat cccgtttact cctccggtct
       61 tttaaaattc tcaccttcat tcagccggtt tatgtccttc atgtcttcgt tcttgctcgg
      121 cagatttctg catccaggcg tgttttcgtt atgcgttttg ctccctttac tcgccagcgc
      181 caccacatca catatctctt tcagctacgc cgcccgccag cggatgcaaa accgtgcgcg
      241 tttattaaaa cagtaccaaa cccatctgaa aaaacaggcc agctacattg tggaaggcaa
      301 tgccgaaagc agaagggcgc tacgccagca caaccgggag cagataaaac agcatccaga
      361 atggtttcct gcgccgctca aggcaagtga cagacgctgg caggcgttgg tagaaaacaa
      421 tcatttcctc agcagcaacc acctgcataa tatcaccgaa gtggcgattc accgtctgga
      481 gcaacagctt ggcaagcctt acgtctgggg cggcacgcgg cccgatcaag gcttcgactg
      541 tagcgggttg gttttttatg cctacaacaa gatccttgag gctaagctcc cgcgcacggc
      601 taatgagatg tatcactacc atcgggcaac cattgtggca aataacgacc tgcgccgggg
      661 agatttgctg tttttccata tccacagccg tgaaattgcc gaccatatgg gcgtgtatct
      721 gggagacggt cagtttattg agtcgccgcg cacgggggaa accattcggg taagtcggtt
      781 agctgaacct ttctggcagg atcatttttt gggcgcgcgg tggatattga cggaagagac
      841 gattttgtag gacggataag gcgtttacgc cgcatccggc ggtggtgcgc agatgcctga
      901 tgcgacgctg acgcgtctta tcatgcctac aaatctgtgc gtttttgcga gccgctttcc
      961 tgttattcca tcacctcatt gccgaccttt ccttttcttc ttaccgttga gaaaaaggag
     1021 tcgccatgtc tgaatatcgt cgttattaca tcaagggggg aacctggttt ttcacggtga
     1081 atttacgcaa tcgtcgaagc caactcttga ccacccagta ccagatgctc cgtaacgcca
     1141 ttattaaagt taagcgagac aggccttttg aaatcaacgc ctgggtcgtt ttgccagagc
     1201 atatgcattg catatggaca ttacctgaag gcgatgatga tttttcctct cgctggcggg
     1261 aaattaaaaa gcaatttacc catgcttgtg gattgaaaaa tatctggcaa ccacgttttt
     1321 gggagcacgc catccgcaat accaaagatt ataggcatca tgttgattat atttatataa
     1381 atccagtaaa gcatggttgg gtaaagcaag tgagtgattg gccattctca acgttccatc
     1441 gcgatgtcgc gcgagggtta tatcccatcg attgggcggg ggacgtaacg gatataaatg
     1501 ctggcgagcg tatcatttta taattatgcg cagatgcctg atgcgacgct ggcgcgtctt
     1561 atcatgccta caaacctgtg ccggatcggt aggccggata aggcgtttat gccgcatccg
     1621 gcggttatgc gcagatgcct gatgcgacgc tggcgcgtct tatcatgcct acaaacctgt
     1681 gctggatcgg taggccggat aaggcgttca cgccgcatcc ggcagttgtg caccaatgct
     1741 ggatacttac atcaacgccc ccataatctt caactccagc tcgtccggca cttcgttata
     1801 cgacagcaca tgcagccccg gcgcaaacaa ccttgcataa cgcgccagca aagggcgcag
     1861 ctgcggtggc accagcagca ccgggtcttt ccccgccgct ttcatctgct ctttcacctg
     1921 tggcatcgta ctctggaact ggttgagcat attcgggtcc actggcacgc tgtcgagcat
     1981 cactttaccg ccctgttgcg cctgattcac taggttagtc agcagatttt ccagctcatt
     2041 attcagggta tacaccgtca gctcctgctt gcgaacgaac ggatgggtaa tgctgcgccg
     2101 cagcgccagg cgcacatcgg ctgccagcag aatatgatct ttggtcaccg cgctactggc
     2161 gaccagcacg gtggcgatgg tgacgatatc gcgcagggaa acgccttcgg tcagcagcgc
     2221 ccggtacact ttcagcaact ggctgtaatt gagcaccgcg cttaaatcct ccgccaggcg
     2281 cggtgccatc gacgacaaac ggttatgcaa ctgcgtaata tcgtcatagt taaataaatc
     2341 aggaatatag ctgcgcacaa tcttatttac atgggtagca atcacgctgg cgctgtcgat
     2401 cacctgatac cccatattca gcaccttcgc tttctgcgca ggctggatcc aggtcaccgg
     2461 catcccgtac gccgggtcgt tccccagcac gccgtctatc tcgccgtagg tttcgctgga
     2521 gggctgggcc atcagtttat ccgccggaat atccgcttca tcagccttaa tgccgttgat
     2581 gaaaatagcg tactggctgg gcttaaggcg gaagttttcc cgaatgcgga tctccggcag
     2641 cagcacgccg ttgccgtcgg atatcacctg tcgcacgccg cgaatacgct gggtgagcgg
     2701 gttgccctgg gctttatcga ccagcgccac cagcttgtag ccgaggctta aactgatagg
     2761 ctcgataagc ggaatggttt cccaactgac ctgttgctcg ctggtttcgg tgatggtgcg
     2821 ggtcagcgtt tcgagacttt tctcttccgc ttccgccgcc tgcgggcgtt tgctcatccg
     2881 ccagccggta aagccaagca gggcgctgaa cagcaaaaac ggcagatgtg gcattcccgg
     2941 cactaccgcc agtacgaaca taattccggt agcggtgtag agcaccgatg ggcttgccag
     3001 caactggtta cgcacgtcat gggcgatatc gccgttgtcg ctgacgcggg tgaggataat
     3061 cgccgccgcg gtggagagta gcagggaagg gatttgcgcc accagcccgt cgccgatggt
     3121 catcagcaca tactgctgga aggcggcgtc ggcgctcagg ttgtatttga agatcccgat
     3181 acagacgccg ccgatcaggt tgatcgccag aatcatcatc ccggcgatag catccccgcg
     3241 cacaaatttc gatgccccgt ccatcgcgcc gtagaaatcg gcctcgctgg caacatcttt
     3301 acgtcgggtt tgtgcctgcg cctgattgat caacccggca ttaagatcgg cgtcaatcgc
     3361 catctgctta ccgggcatcg catccagggt aaagcgggct gaaacctcgg aaatacgctc
     3421 ggcccctttg gtgacgacga taaagttgat tgtcaacagc gtcagtaaat ccgagcgtga
     3481 aagcattatc gccgcgctgc acgggcagtc gatttttaac ggtggtgggc tgtcgccgtt
     3541 aaataaaatc agcccgtctc atccgccaaa acctgccacc gtggcggtgc cggaagaaac
     3601 ggagaaaaaa gcgcgcgacg tcaatgagaa aacggcgctg ctgaagaaga aatccgccac
     3661 cgagcttggc gagctggcaa ccagtatcac caccattgcc cgtgatgcgc atatggaagc
     3721 gaatctggag atggagattg ttccccaggg attacgcgtg ctgattaaag acgaccagaa
     3781 tcgcaatatg ttcgaacgcg gcagcgcgca gattatgccg ttctttaaaa cgctgctggt
     3841 ggagctcgcg ccagttttcg actcgctcga taacaaaatt attattaccg ggcatatcga
     3901 tgcgatggct tacaaaaaca atatctacaa caactggaac ctttcgggtg accgcgcgct
     3961 ttcggctcgt cgggtgctgg aagaggccgg aatgccggaa gataaagtga tgcaggtaag
     4021 cgcaatggcg gaccagatgc tgctggattc caaaaatccg caaagcgcgg gcaaccggcg
     4081 cattgagatt atggtgctga ccaaaagtgc gtccgatacg ctgtatcaat actttggtca
     4141 gcatggggat aaagtggtgc agccgctggt gcaaaagctg gataagcagc aggtgctttc
     4201 gcagcgaacg cgttaaatgc tgaatcttta cgcatttctc aaactctgaa atcactgtat
     4261 actttaccag tattgagagg tgagcaatgc gtaaaatcat gattttcact tcctaaaaca
     4321 ccccttataa tctggtcata gagtcattat agcactggat gaatatacag atgaggaagg
     4381 tttacagtta cattcgcttt tcgtcaacga aacaagcttt tggtgatagt catcgaagac
     4441 agagtaaggc catacaggat tggttagcca gccatccaga ccacatccta gacgaatctc
     4501 ttagttttga agatttagga cgttcagcct ttcacggtga ccatctaaaa gaaggtggag
     4561 ca
//
LOCUS       DADLFA010000231         4518 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000231 DADLFA010000000
VERSION     DADLFA010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4518
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.233"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            151..672
                     /locus_tag="JQH65_003748"
     CDS             151..672
                     /locus_tag="JQH65_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HAY6141009.1"
                     /translation="MLHYSGGLKYRCHLSDMENNMRKYIPLVLFIFSWPVLSADIHGR
                     VVRVLDGDTIEVMDSLKAVRIRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQ
                     RDRYGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSD
                     ADPVPPWIWRHRK"
     gene            718..927
                     /gene="hha"
                     /locus_tag="JQH65_003749"
     CDS             718..927
                     /gene="hha"
                     /locus_tag="JQH65_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HAY6141010.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            966..1556
                     /locus_tag="JQH65_003750"
     CDS             966..1556
                     /locus_tag="JQH65_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HAY6141011.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQTTLLTEREVSFMKGLFRIVDMTRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            1796..2056
                     /locus_tag="JQH65_003751"
     CDS             1796..2056
                     /locus_tag="JQH65_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HAY6141012.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            2280..2354
                     /locus_tag="JQH65_003752"
     CDS             2280..2354
                     /locus_tag="JQH65_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HAY6141013.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            2347..3204
                     /gene="repA"
                     /locus_tag="JQH65_003753"
     CDS             2347..3204
                     /gene="repA"
                     /locus_tag="JQH65_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HAY6141014.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            complement(4257..>4518)
                     /locus_tag="JQH65_003754"
     CDS             complement(4257..>4518)
                     /locus_tag="JQH65_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAY6141015.1"
                     /translation="QAAAFLGVVPVEKYSGTSVRGCPKLSKIGPPEIRAKLYLASLCG
                     LRFNPLMKAMYERLCLRGKAKMCAIGALMRKLVHWCYGVLKT"
BASE COUNT         1214 a         1104 c         1121 g         1079 t
ORIGIN      
        1 gaaggctgaa aggagtttat gagatttgct tcttaatatc cggtcgggca gtatgaccgg
       61 acagttctga gtagcacaga gtcttttccg cattaagtcg attttttata ttttgtgatt
      121 aatttcacaa aataaggtgt tattcaatgt gtgctgcact attcaggggg gctgaaatac
      181 aggtgccatt tatctgatat ggagaataac atgaggaaat atattccact ggtattattt
      241 atcttttcat ggccggtatt gtctgcagat attcatggac gggttgttcg tgtactggac
      301 ggtgatacca tcgaggtcat ggactcactg aaggcggtgc ggatcaggct ggtaaatatt
      361 gatgcaccgg aaaagaaaca ggattatggc cggtggtcaa cagacatgat gaaatcactg
      421 gttgcaggaa aaacggttac cgtcacatat tttcagcgag atcgttatgg tcgtattctc
      481 gggcaggttt atgcaccaga cggaatgaat attaatcagt ttatggtccg ggccggggct
      541 gcctgggtat atgagcagta caacacagat cccgtgctac ctgtccttca gaacgaagcc
      601 agacaacaaa aacgtgggct ctggtcagat gctgatccgg ttcccccctg gatatggagg
      661 caccgtaaat aaagcgatgg caatcctatc attcatctgt tctcagcggg aataaaaatg
      721 gcaaaaacaa aacaggaatg gctgtatcag ttacggcgat gttcatcagt caatacgctg
      781 gaaaaaatta ttcataaaaa cagagacagt ttatcaaaca gtgaacgtga atcatttaat
      841 tcagcagcag accaccggct agcggaatta atcaccggaa agttatatga caggatacca
      901 aaagagatat ggaaatatgt aaggtaataa ttcagcaata attaattgcc aggagcagat
      961 aaataatgaa gttgattatt tttattctca ttgttctgat tattgcagcg ctacttatcc
     1021 ggattatcct cagaagtgta aatcagcatt ctcctttgct gatgcaattg catgcggcag
     1081 gtatacggac cggtgatgca gagcgaatac tgtccagcgg tgaatactgg caacgtcaga
     1141 cgacgctgct gacagaaagg gaagtcagtt ttatgaaagg actgttcaga attgtggata
     1201 tgacgcggtg gtatctgtgt ccgcaggtac gggtcgcgga tatcgtccag ctgaacggga
     1261 atatccggcc acgatcacgc cagtggtggc agttattcag gatggtgtct cagtggcatg
     1321 ttgatgtggt tatcgttgag ctgcgttcgt tcagtattgt tgcagcagta gagctggatg
     1381 atgccagcca tttacgaccg gaacgcagac gccgggatat tcttctggaa gaggttctga
     1441 ggcaggctgg tattccgttg ctcagaagcc acgatgccag aaaactgctg cagatgaccg
     1501 gagaatggct gaatacaaca ggggctgatc agcagtcacc ggaacatcgt agctgacgcc
     1561 ttcacgttgc tcagttgtcc aaccccggaa acgggaaaaa gcaagttttc ctcgctcccg
     1621 gcgtttccat aactgaaaac catactattt cacagtttaa atcacattaa acgacagtaa
     1681 tccccgttga tttgtgcgcc aacacagatc ttcgccacaa ttctcaagtc gctgatttca
     1741 aaaaactgta gtatcctctg cgaaacgatc cctgtttgag tattgaggag gcgagatgtc
     1801 gcagacagaa aatgcagtga cttcctcatt gagtcaaaag cggtttgtgc gcagaggtaa
     1861 gcctatgact gactctgaga aacaaatggc cgctgttgca agaaaacgtc ttacacacaa
     1921 agagataaaa gtttttgtca aaaatcctct gaaagatctc atggttgagt actgcgagag
     1981 agaggggata acacaggctc agttcgttga gaaaatcatc aaagatgaac tgcagagact
     2041 ggatatacta aagtaaagac tttactttgt ggcgtagcat gctagattac tgatcgttta
     2101 aggaatttta tggctggcca cgccataagg tggcagggaa ctggttctga tgtagatgta
     2161 caggagccag aaaagcaaaa accccgataa tcttctttaa ctttggcgag tcagaaagat
     2221 taccggggcc cacttaaacc gtatagccaa caatcaagct atgcggggag tatagttata
     2281 tgcccggaaa agttcaagac ttctttctgt gctcgctcct tctgcgcatt gtaagtgcag
     2341 gatggtgtga ctgatcttca acaaacttat taccgccagg taaagaaccc gaatccggtg
     2401 ttcacacccc gtgaaggtgc ccggacgctt ccgttctgcg ggaaactgat ggaaaaggcg
     2461 gtgggcttca cctcccgttt tgatttcgcc attcatgtgg cacatgcccg ttcgcttggt
     2521 ctgcgtcggc gcatgccacc ggtactacgt cgccgggcca ttgatgcgct gctgcagggg
     2581 ctgtgttttc actatgaccc actggccaac cgcgtccagt gttccatcac cacgctggcc
     2641 attgagtgcg gactggcgac ggagtctgct gccggaaaac tctccatcac ccgggccacc
     2701 cgggccctga cgttccttgc agagctggga ctgattacct accagacgga atatgacccg
     2761 cttatcgggt gctacattcc gaccgatatc acgttcacat ctgcactgtt tgctgccctc
     2821 gatgtatcag aggaggcagt ggccgccgcg cgccgcagtc gtgtggaatg ggaaaacaga
     2881 cagcgcaaaa agcaggggct ggataccctg ggtatggatg aactgatggc gaaagcctgg
     2941 cgttttgttc gtgagcgttt ccgcagttac cagacagagc ttaagtcccg tggaatgaag
     3001 cgtgcccgtg cgcgtcgtga tgcggacagg cagcgtcagg atattgtcac cctggtgaaa
     3061 cggcaactga cgcgtgaaat ctcggaaggg cgcttcactg ccagtcgtga ggcggtaaaa
     3121 cgtgaagtgg agcgtcgtgt gaaggagcgc atgattctgt cacgtaaccg taattacagc
     3181 cggctggcca cagcttcccc ctgaaagtga cctcctcaga ataatccggc acgcgccgga
     3241 ggcattcgca cgcctgaagc ccgccagtgc acaaaaaatc agcgctacat acaaaaaaca
     3301 acctcatcat ccaccttctg gtgcatccgg tcccccctgt ttttgataca aaacacgcct
     3361 cacagacggg gaattttgct tatccacatt aaactgcaag ggacttcacc ataaggttac
     3421 aaccgttcac gtcataaagc gccagccgtc agccctacag ggtgcaatgt atcttttaaa
     3481 cacctgttta tatctccttt aaactactta attacattca tttaaaaaga aaacctgttc
     3541 actgcctgtc ctgtggacag acagatatgc acctcccacc acaagcggcg ggccccgacc
     3601 gaagccggtt tagttacaac atgcaaacat aaacccctaa aaaacccaag tccgacgcag
     3661 aactgaaacc acaaagcccc tccctcataa ctgaaaagcg gccccgcccc ggcccgaagg
     3721 gccggaacag agtcgctttt aattatgaat gttgtaacta tattatcatc gctgtcagtc
     3781 ctctggctgg acgtatcgag tacacgctcg taagcggccc tcacggccct cacggcccgc
     3841 taacgcggag atacgccccg actgcgggta aacccttgtc gggctccgac cgcgcacaga
     3901 agctctgtca tagctgaaaa cgggtatggc ttggcagcta ccatgttctg tcaagaaaaa
     3961 tcgcttgaac tccgacgtac agccccctaa gtactgccag caagctgtca gcactaagtc
     4021 cccgatacag cgcggcatag ccgcgctaac ggggttaaaa gggcttacct atacttcatc
     4081 atcgctgaaa gcccgcatgg cctcgcaggg cagagatggg ataggtgaaa cctatcaata
     4141 cgtaccggct tacgccgggc ttcggcggtg ttactcctgt accatatgta actacatcat
     4201 gccgccttac atgcgctacg cgcatatgaa gacaaatagc ccacatcaaa cggtttttag
     4261 gtcttcagta cgccatagca ccagtgcacg agttttctca tcagcgcacc aatggcacac
     4321 attttggcct tacctctcag gcataatcgc tcatacatcg ctttcatcaa tgggttaaat
     4381 ctcagaccac acaacgaagc cagatacagt ttcgcccgga tttccggcgg accaattttt
     4441 gacagtttcg gacaccctct gaccgaggtg ccggaatatt tttcaacggg aacgacacct
     4501 aaaaatgcag ccgcctgt
//
LOCUS       DADLFA010000232         4436 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000232 DADLFA010000000
VERSION     DADLFA010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4436
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.234"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..1088
                     /gene="nadB"
                     /locus_tag="JQH65_003755"
     CDS             <1..1088
                     /gene="nadB"
                     /locus_tag="JQH65_003755"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HAY6141016.1"
                     /translation="VGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPDISSGD
                     GIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTRFMPDF
                     DERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLGLGIDL
                     TQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSLLECLV
                     YGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDYVGIVR
                     TTKRLERALRRINMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRKESRGL
                     HFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(1073..1810)
                     /gene="trmN"
                     /locus_tag="JQH65_003756"
     CDS             complement(1073..1810)
                     /gene="trmN"
                     /locus_tag="JQH65_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HAY6141017.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWLTQQTVRFDLIISNPPYYQQGVECAIPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            1942..3276
                     /gene="srmB"
                     /locus_tag="JQH65_003757"
     CDS             1942..3276
                     /gene="srmB"
                     /locus_tag="JQH65_003757"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HAY6141018.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIEELRPKTRTPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(3485..4366)
                     /locus_tag="JQH65_003758"
     CDS             complement(3485..4366)
                     /locus_tag="JQH65_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY6141019.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKGSDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCIEECFLPG"
BASE COUNT         1125 a         1209 c         1125 g          977 t
ORIGIN      
        1 ttgtaggcgc gtgggtatgg aaccgaaata aagaaacggt ggaaacctgc cacgcaaaag
       61 cagtggtact ggcaaccggc ggtgcgtcga aggtgtatca gtacaccacc aatccggata
      121 tttcttctgg cgatggcatt gctatggcgt ggcgcgcggg ctgtcgggtt gccaatctcg
      181 aatttaatca gttccaccct accgcactgt atcacccaca ggcacgcaat ttcctgttaa
      241 cggaagcact gcgcggcgaa ggcgcttatc tcaagcgccc ggatggcacg cgttttatgc
      301 ccgattttga tgagcgcggc gaactggccc cgcgcgatat tgtcgcccgc gccattgacc
      361 atgaaatgaa acgcctcggc gcagattgta tgttcctcga catcagccat aagcccgccg
      421 attttattcg ccagcatttc ccgatgattt atgaaaaatt gctcgggctg gggattgatc
      481 tcacacaaga accggtaccg attgtgcctg ctgcacacta tacctgcggt ggtgtaatgg
      541 ttgatgatca tgggcgtacg gacgtcgagg gcttgtatgc cattggcgag gtgagttata
      601 ccggcttaca cggcgctaac cgcatggcct cgaattcatt gctggagtgt ctggtctatg
      661 gctggtcggc ggcagaagat atcaccagac gtatgcctga tgcccacggc gtcagtacgt
      721 taccaccgtg ggatgaaagc cgcgttgaga accctgacga acgggtagta attcagcata
      781 actggcacga gctacggctg tttatgtggg attacgtcgg cattgtgcgc acaacaaaac
      841 gtcttgagcg cgccctgcgg cgtatcaata tgttgcaaca ggaaatagac gaatattacg
      901 cccacttccg cgtgtcgaat aatttgctgg agctgcgtaa tctggtgcag gttgcggaat
      961 tgattgttcg ctgtgccatg atgcgcaagg agagtcgtgg cctgcatttc acactggatt
     1021 atccggaact gctcacccat tccggtccgt cgatcctttc ccccggcaat cattacataa
     1081 acagataaaa agcctgggtc agcgccgtat acgcttcgga atagttctgg tctggcccac
     1141 gaatgactaa gcgatcgcta aagcattctc ccgcctgcgg ggagaatgcc agcagcaccc
     1201 gatgcggcag tcgcgcttcg ttttccgcca catccgtccg caaacgtaaa tgccagccca
     1261 tgcttaatgc cagctccgta aaaccattac caatctgctc tggcagcact acgcagaaaa
     1321 atccctcttc ggtaatgcac tccgccgcac aggtcagcag cgatgggtga tcaagcgtag
     1381 tggtatagcg agcctgttcc cgttgaggga tcgcgcactc tactccctgc tgatagtaag
     1441 gtgggttact gatgattaaa tcgaagcgta ctgtctgctg tgtgagccac tgctgaatat
     1501 ccgccgtatg gacgttaatc cgctctgccc acggggactg gttgatattt tcctgcgcct
     1561 gcgcggcagc ttcactttcc agctcaacgg catcaattat cacgctgtca ctggttcgct
     1621 gcgccagcat cagtgccagc aacccgctac ccgcgccgat atcaaggcaa cgtttcaccc
     1681 cagccaccgg tgcccatgcg cccagcaaaa tgccatccgt tcccactttc atcgcacagc
     1741 gatcgtgagc aacaaaaaac tgtttaaaag taaatccatt acgacgaagc acggatgtag
     1801 actgtgacat gaaaataaaa ccttgcagga aaaacggcga tagcaccggg tgagaacaat
     1861 acccgagaag cgatatccat acaaacagat gaagattgca gccgtaacgt ctataatcag
     1921 cgccccacac agaggtagaa catgactgta acgacttttt ccgaacttga actcgacgaa
     1981 agcctgctgg aagccctcca ggataaaggt ttcactcgcc cgaccgccat tcaggctgcc
     2041 gccattccgc ctgcgctcga tggccgtgat gtactcggtt ctgcgccgac aggcaccggt
     2101 aaaacggcgg cgtatctgct gccagcgttg cagcacctgc tcgatttccc gcgtaagaaa
     2161 tccggtccgc cgcgtatttt gatcctcacc ccaactcgcg agctggcgat gcaggtggcc
     2221 gatcatgccc gcgaactggc gaaacatacg catctggata tcgccaccat taccggcggc
     2281 gtagcctata tgaaccacgc ggaagtgttc agcgaaaatc aggacatcgt ggtcgccacg
     2341 accggacgtc tgctgcaata cataaaagaa gagaacttcg attgccgcgc ggttgaaacg
     2401 ctgatcctcg acgaagcaga ccgtatgctg gatatgggct tcgctcagga tatcgaacat
     2461 attgctggcg aaacgcgctg gcgtaaacag accctgctct tttcggcaac gctggaaggc
     2521 gatgcgattc aggactttgc cgagcgtctg ctggaagatc cggtggaggt ttctgccaat
     2581 ccgtccaccc gtgagcgcaa aaaaattcat cagtggtatt accgcgccga tgacctggaa
     2641 cataaaaccg cattgctggt gcatctgtta aaacagccgg aagcgacccg ctcaattgtg
     2701 tttgtgcgta agcgtgaacg tgtgcatgag ctggcaaact ggctgcgcga agcgggcatc
     2761 aacaactgct atctcgaagg tgagatggta cagggcaagc gtaatgaagc gattaaacgc
     2821 ctgaccgaag gccgcgtaaa cgtactggta gcaaccgatg ttgccgcgcg cggtatcgac
     2881 attccagacg tcagccacgt ctttaacttc gatatgccgc gcagtggtga tacttatttg
     2941 caccgtatcg gacgtaccgc gcgcgccggt cgtaaaggca ccgcgatttc gctggtggaa
     3001 gcccatgacc atctgctgct tggcaaagtt ggccgttata ttgaagagcc gataaaagct
     3061 cgcgtcattg aggaactacg cccgaaaacg cgtacgccaa gcgagaagca gaccggcaag
     3121 ccatcgaaga aagtactggc aaaacgcgcc gagaagaaaa aagctaaaga gaaagagaag
     3181 ccgcgggtga aaaaacgcca tcgcgacacc aaaaatattg gtaagcgccg taaaccgagc
     3241 ggaacgggcg tgccaccgca aacgacagaa gagtaatctt aattccgggt ttaagcccgg
     3301 aattaaaagt gctggcaaac gcaaaactgc ctgatgcgct acgcttatca ggcctacatg
     3361 gctcatgcaa tatattgaat ttgcacgatc ttgtaggccg gataaggcgt tcacgccgca
     3421 tccggcatga acaaaacgca cttcgtcgac aagttgggtg ccgggtttaa gcccggcatt
     3481 atttttatcc cggcaaaaaa cactcttcaa tacactgaat aaaagtgtgc atcgccgggc
     3541 taaccgcttt tccagcatgg tgtgcgcaca tagccgtaat ggtctgcgac tgttcgccaa
     3601 aaggcaattc aattaattct ccgctctcaa gctctttttc caccgcaaag cggggcaagt
     3661 aactgacacc gatattcgcc gcgacacaac gtttgatact ttcaatgctt aacagctcaa
     3721 tggtgttttc caccgtgatc cgccgctgac gcagcgtgct ctcaaatatc tgccggaaga
     3781 cacattgcgg ttcgttgata ataaagctac aggcgttatg tcttcccggc tcagtaaaat
     3841 cgacatctgc aatttgcggt gaagccacca gcaccagcga ttgttcaccc aactctcgtc
     3901 ggttcagggc atcatcattc cctacgcgat aaaagacgcc gacatcggcc tcatcattca
     3961 gtagcgcatc acggatcacg taacagttca gcgactgcaa cgataaacgc acttttggtg
     4021 cgcgttgccg aaaacgctgc aacacctgcg gcattcggta agagagcaac gtttcgcccg
     4081 aaacaacgcg tagttccccg tccggatctg accccttttt ggcagcctca cgaagcgtat
     4141 ccatcactcg ggtaagttca taaatgtgcg gcagcaactt ttttccttcg cgggtaaggc
     4201 acatccgtcg gccaattttc tcaaataact ggactgaaaa ctcctgctca agctgctgaa
     4261 tatgaaaagt caccgtcgat tgtgtacagc acaatttttg cgaagctcgc aaaaaggaac
     4321 cctcttccac cacggtttta agcgtaataa aacggcgcag atccataacc ccgaacctat
     4381 cgaaaatatc gaatctagaa tataaaaaca ttcatttttt taaatgttct gtgtcg
//
LOCUS       DADLFA010000233         4429 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000233 DADLFA010000000
VERSION     DADLFA010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4429
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.235"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            127..456
                     /locus_tag="JQH65_003759"
     CDS             127..456
                     /locus_tag="JQH65_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HAY6141020.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            704..949
                     /gene="nrdH"
                     /locus_tag="JQH65_003760"
     CDS             704..949
                     /gene="nrdH"
                     /locus_tag="JQH65_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAY6141021.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            946..1356
                     /gene="nrdI"
                     /locus_tag="JQH65_003761"
     CDS             946..1356
                     /gene="nrdI"
                     /locus_tag="JQH65_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAY6141022.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            1329..3473
                     /gene="nrdE"
                     /locus_tag="JQH65_003762"
     CDS             1329..3473
                     /gene="nrdE"
                     /locus_tag="JQH65_003762"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAY6141023.1"
                     /translation="MATTTAECLTQETMDYHALNTMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAYAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNCASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTRDMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            3483..4397
                     /gene="nrdF"
                     /locus_tag="JQH65_003763"
     CDS             3483..4397
                     /gene="nrdF"
                     /locus_tag="JQH65_003763"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAY6141024.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPYALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYSDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSRIGN"
BASE COUNT         1094 a         1132 c         1128 g         1075 t
ORIGIN      
        1 cgggcctacc ttacctgatt gcgcattcac aacgctttca gaaaagtcca taaagccgcg
       61 atgaacctca gttaagaaaa ttatggtcta cactgaaaat tacatcgaat tctaatgggg
      121 gatcatatgt ttaaccgacc gaaccgcaac gacgttgatg atggcgtgca ggatatccag
      181 aatgatgtca atcaattagc tgacagcctc gaatctgtat tgaagtcctg gggcagcgac
      241 gccaaagggg aagctgaagc cgcacgcagt aaagctcagg cgttgctgaa agaaaccagg
      301 gcgcgaatgc atggtcgtac tcgcgtccag caagccgcgc gcgatgccgt tggctgcgcg
      361 gattcttttg ttcgtgaaag accctggtgt agcgtgggta cagcagctgc ggtaggtatt
      421 tttatcggcg cactgttaag catgcgcaaa tcgtagtgca aaaatgataa taaatacgcg
      481 tctttgaccc cgaagcctgt cttcggggtt tctttttgcc tggtgaatca caaaaatccc
      541 cctcccccgt cacgctcata tccagggtaa tttcgatcac tatttgctat atattgtgta
      601 gttgaatctt ttttcaacta catctagtat ctctgtatca acagagagac aacccgacgc
      661 gtatcatcgc gccgtatctt cattttaaac ggaaatacga atcatgcgca taactattta
      721 cactcgtaac gattgcgttc agtgccacgc caccaaacgg gcgatggaaa accgggggtt
      781 tgattttgaa atgattaatg tcgatcgcgt tcctgaagca gcggaagcgt tgcgtgctca
      841 gggctttcgt cagttgccgg tagtgattgc tggcgatctt agctggtctg gtttccgtcc
      901 ggacatgatt aaccgtctgc atccagcgcc acacgcggcc agtgcatgag ccagctcgtc
      961 tacttctcca gcagctccga aaacacgcag cgttttatcg aacgtttagg tctgcccgcg
     1021 gtgcgcatcc cgctcaatga gcgggaacgg attcaggtag acgagcctta catcctgatc
     1081 gtgccctctt acggcggcgg cggtacggct ggcgcggtgc cacgacaggt aattcgcttt
     1141 ttaaacgacg agcacaaccg ggcgttgctt cgcggcgtta ttgcttctgg taatcgcaac
     1201 tttggtgagg cgtatggccg cgccggagat gtgattgccc ggaaatgcgg cgtgccgtgg
     1261 ctgtaccgtt ttgaactcat gggtacgcaa agcgatatcg aaaacgttcg taaaggagta
     1321 accgaatttt ggcaacgaca accgcagaat gcctgacgca ggaaacgatg gattaccacg
     1381 cgctgaatac gatgcttaac ctctacgata gcgcaggtcg cattcagttc gataaagacc
     1441 gccaggccgt tgacgccttt atggcgacgc atgtgcgtcc gaacagtgtg accttcagta
     1501 gccagcagca gcgcctgaac tggctggtca acgaaggtta ctatgatgaa agcgttctta
     1561 atcgctactc tcgcgacttt gtcattacgc tgtttgccta cgcacacacc agcggttttc
     1621 gtttccagac attcctcggg gcatggaagt tttacaccag ctatacgttg aagacattcg
     1681 acggtaaacg ttatctggaa gattttgccg atcgagtcac gatggtggcg ctgacgctgg
     1741 cacaaggcga tgcgacgctg gcgttgcaac tgaccgatga aatgctgtca ggacgctttc
     1801 agccagccac tccgacattc ctcaactgcg gtaagcagca gcgcggcgaa ctggtttcct
     1861 gttttctgct gcgtattgaa gacaatatgg agtcgattgg ccgggcggta aattccgcac
     1921 tgcaactgtc gaaacgcggc ggcggcgtgg catttttgct gtcgaatctg cgagaagcgg
     1981 gcgcgccaat taaacgtatt gaaaatcaat cttctggcgt aattccggtg atgaaaatgc
     2041 tggaagacgc attttcctat gccaaccaac tcggcgcgcg tcagggggca ggtgcagtct
     2101 atttacatgc tcatcatccc gatattctgc gttttctcga tacaaaacgg gaaaatgccg
     2161 acgaaaaaat ccgcattaaa acactgtcgc tgggcgtggt gatcccggat atcactttcc
     2221 atctggcaaa agagaatgcg cagatggcgc tgttttcgcc ctatgacgtt gagcgagttt
     2281 atggcaagcc gtttgccgat atcgccatca gcgaacacta tgacgaactg gttgccgatg
     2341 aacgcatccg caaaaaatac ctcaacgccc gtgatttctt ccagcggctg gcagaaatcc
     2401 agtttgagtc cggctatccc tacatcatgt atgaagacac ggtaaaccgt gctaaccctg
     2461 tcgccgggcg cataaatatg agtaatctct gctcagagat tttgcaggtc aactgtgcct
     2521 cagagtatga cgagaatctc gactatgccc gcacaggcca tgatatctcc tgcaatttag
     2581 gttcgttgaa tattgcgcac accatggatt cccccgattt tgcccgcacg gtagagactg
     2641 ccgtgcgcgg tttaacggcg gtgtcagata tgagtcatat ccgcagcgtg ccgtccatcg
     2701 aagccggaaa tgccgcctca cacgccatcg gactggggca gatgaattta cacggctatc
     2761 tggcgcgaga aggcatcgct tatggttcgc cggaagcact ggatttcacc aatctctatt
     2821 tctatgccat cacctggcac gcactgcgta cctcgatgtt gctggcgcgc gaacgcggtg
     2881 aaaccttcgc cgggttcaaa cagtcacgtt atgccagtgg tgaatatttt agccaatatc
     2941 tgcaaggtaa ctggcagccg aaaacggcga aagttggcga actgtttgcc cgcagcggta
     3001 ttacgttacc tacccgtgat atgtgggcgc aactgcgcga cgacgtgatg cgctacggca
     3061 tttataacca gaatcttcag gcggtgccgc caaccggttc tatctcttat atcaaccatg
     3121 ctacgtcgag tattcatccg attgtggcga aagtagagat acgcaaagag ggcaaaacag
     3181 gacgcgttta ctaccctgcc ccgtttatga ctaacgagaa tctggcgctg tatcaggatg
     3241 cttacgaaat cggcgcagaa aagatcatcg acacctacgc cgaagcgact cgccatgtcg
     3301 atcaggggct gtcgctgacg ctgtttttcc ccgataccgc caccactcgc gatatcaaca
     3361 aagcgcagat ttacgcctgg cgcaagggta tcaaaacgct ctattacatt cgcctgcgtc
     3421 agatggcgct ggaaggcact gaaattgaag gctgcgtctc ctgtgcactt taaggaatat
     3481 ctatgaaact ctcacgtatc agcgccatca actggaacaa gatatctgac gataaagatc
     3541 tggaggtgtg gaatcgcctg accagcaatt tctggctacc agaaaaggtg ccgctgtcga
     3601 acgatattcc tgcctggcag acattaactg tcgtagaaca acaactgacg atgcgcgttt
     3661 ttactggcct gacgctgctc gacacgctgc aaaatgttat cggcgcgcct tcgctgatgc
     3721 cctatgcact cacgcctcat gaagaagcgg tattatcgaa tatcagcttt atggaagcgg
     3781 ttcatgcccg ctcttacagt tcgattttct cgacgctgtg tcagaccaaa gatgtcgatg
     3841 ccgcctacgc ctggagtgaa gaaaacgcac cgttgcagcg aaaagctcag attattcagc
     3901 aacattatcg cggtgatgat ccgctgaaaa agaaaatcgc cagtgtgttt cttgaatctt
     3961 ttttgttcta ttccggtttc tggctgccga tgtatttttc cagccgcgga aagctgacca
     4021 ataccgcaga tctgatccgt ctgattatcc gcgatgaagc agtccacggt tactacatag
     4081 gctataaata tcagaaaaac atggaaaaga tatctctggg acaacgtgaa gagttgaaga
     4141 gtttcgcctt cgatttgttg ctggaactct acgacaacga gttgcaatac agcgatgagc
     4201 tgtacgccga aaccccgtgg gctgacgatg tgaaagcgtt tctctgttac aacgccaata
     4261 aggctttgat gaatctgggc tacgaaccgt tatttcccgc agaaatggcg gaagtgaatc
     4321 cggcaatcct cgccgcgctt tcgccgaatg ccgatgaaaa tcacgatttc ttttccggtt
     4381 cacgtatagg aaattgaaaa actaagcgat acccctgtcc gcgccttat
//
LOCUS       DADLFA010000234         4415 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000234 DADLFA010000000
VERSION     DADLFA010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4415
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.236"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..203)
                     /locus_tag="JQH65_003764"
     CDS             complement(<1..203)
                     /locus_tag="JQH65_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141025.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGTPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPG"
     gene            431..1039
                     /locus_tag="JQH65_003765"
     CDS             431..1039
                     /locus_tag="JQH65_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HAY6141026.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1137..2528
                     /gene="selA"
                     /locus_tag="JQH65_003766"
     CDS             1137..2528
                     /gene="selA"
                     /locus_tag="JQH65_003766"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HAY6141027.1"
                     /translation="MTTETRSLYSQLPAIDRLLHDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2525..4369
                     /gene="selB"
                     /locus_tag="JQH65_003767"
     CDS             2525..4369
                     /gene="selB"
                     /locus_tag="JQH65_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HAY6141028.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPSFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPGREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFSDEPWW
                     VRDLAKETGTDEQAMRLTLSQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
BASE COUNT         1010 a         1128 c         1276 g         1001 t
ORIGIN      
        1 ccgggcaggg cgatgtcggt ttcaccggga aggacctttg caccgagcag gggattgacc
       61 ggatggccgg acgtcactcc gccggggcac accgaacagg ccacgccggt gggggtacca
      121 atgcgcaccc cggctgaacc ctgaacaatg ctaccgccat actgcgtcat gtcgccctga
      181 cgcgctgccg gttttccgct catttctcat ccttgtttta tcgggttatt tgtctgctga
      241 caggcaaaac cctacagatt tataagaaag cctgaagtga gatatccatc agagtgaaat
      301 aattaggaaa atatttatat caccatgata aatatgtgat ttatttggaa gtaacaaacc
      361 atcaacattt tctgcccttt ttcccatcac caggccatac tgatattttt cctcgcacaa
      421 aggactcggc atgaaactcg tcggtagcta caccagcccg tttgtacgca aactttctat
      481 tctgttgtta gaaaagggca taactttcga atttattaat gaactgccct ataacgcgga
      541 caacggcgtg gcgcaattta acccgttagg aaaagtgccg gtgctggtga ccgaagaggg
      601 cgaatgctgg tttgattcgc cgatcatcgc tgaatatatt gagttaatga atgtcgctcc
      661 ggcgatgttg ccgcgcgatc cgctggagtc gttgcgggtg cgcaaaattg aggcgctggc
      721 ggatggcatt atggatgccg ggctggtatc ggtgcgtgaa caggcgcgtc cggcggcgca
      781 gcagtctgaa gatgaattgt tacgccagcg ggagaaaatc aaccgcagtc tggatgtgct
      841 ggaaggatat ctggtcgatg gcacactcaa aaccgatacg gtcaatctgg cgactatcgc
      901 cattgcctgt gctgtcggat atctcaattt ccgccgcgtt gcgccaggtt ggtgtgtcga
      961 ccgcccacac ttagtcaaac tggtcgaaaa cctgtttagc cgcgaaagtt ttgctcgcac
     1021 cgaaccgcca aaggcttgat gcgcgatatg tcctcctgac ccatctcacg ttacaatccg
     1081 tggttatgtt aaacgccctt ctccatgtga gagggccttg attagccagg tttcctatga
     1141 caaccgaaac gcgttccctc tatagtcaac ttccggctat tgatcgctta ttgcacgata
     1201 gctccttcct ttctttgcgt gatacttatg gtcacacccg cgtggtggaa ttgttgcgtc
     1261 agatgctcga cgaagcgcga gaagtgattc gtgacagcca gacgcttcct gcgtggtgtg
     1321 aaaactgggc gcaagaagtc gatgcccggt tgacgaaaga ggcgcagagc gcgctgcgtc
     1381 cggtgatcaa cctgacggga accgtgctgc ataccaacct tggacgagct ttacaggcgg
     1441 aagccgcggt ggaagccgtt gcgcaggcta tgcgttcgcc agtgaccctc gagtacgatc
     1501 tggacgacgc tggacgcgga catcgcgatc gggcgctggc gcaactgctg tgccgtatta
     1561 cgggggcgga agatgcctgt atcgtcaata acaatgcggc ggcggtgtta ttgatgttgg
     1621 cggccactgc cagcgggaaa gaggtggtag tatctcgcgg cgaattggtg gagattggcg
     1681 gcgcgtttcg tattcccgat gttatgcgtc aggcaggctg caccctacac gaagtaggga
     1741 ccaccaaccg cacgcacgcg aatgattatc gtcaggcggt gaatgaaaat accgcactgt
     1801 tgatgaaagt acataccagt aactacagca ttcaggggtt caccaaagcg atagatgaag
     1861 cggaactggt ggcgctcggc aaagagctgg atgttcccgt agtgactgat ttaggcagtg
     1921 gctcgctggt cgatcttagc cagtacggtt tgccgaaaga gccaatgccg caggagttga
     1981 ttgcggcggg cgtcagtctg gtgagcttct ccggcgacaa gttgttaggc gggccgcagg
     2041 cagggattat cgttggtaaa aaagagatga tcgcccgcct gcaaagccac ccgctgaagc
     2101 gtgcattacg cgcggataaa atgaccctcg cggcgctgga agccacgttg cgtctttatt
     2161 tacaccctga agctctgagt gaaaaattac cgaccctgcg cctgcttacc cgcagcgcag
     2221 aggtcattca aatccaggca caacgtttac aggctcccct tgccgcacat tacggcgcgg
     2281 agtttgcggt acaggttatg ccatgtcttt cgcagattgg cagtggttcg ctgccggttg
     2341 atcgcctgcc gagcgcggca ttaacgttta caccccatga tggacgcggt agccaccttg
     2401 agtcattagc cgcccgctgg cgtgaattgc cagtgccggt gattggtcgt atttatgacg
     2461 gacgattgtg gctggattta cgctgccttg aagatgagca acggtttttg gagatgttgt
     2521 tgaaatgatt attgcgactg ccggacacgt tgaccacggc aaaacaacct tattgcaggc
     2581 gattactggc gtaaatgctg accgtctgcc ggaagaaaaa aagcgcggca tgaccatcga
     2641 tctcggctat gcctactggc cgcagccgga tggtcgcgtg cctagtttta tcgacgtacc
     2701 cggtcatgaa aagtttcttt ccaacatgct ggcgggcgtt ggtggtatcg atcacgcgct
     2761 gttggtggtg gcgtgcgatg acggcgtgat ggcgcagacc cgtgagcatc tggcgatttt
     2821 gcagctgacc ggtaacccga tgctgacagt ggcgctgacc aaggccgatc gcgtggacga
     2881 agcgcgtgtt gatgaggttg aacgccaggt aaaggaggtg ctgcgggaat acggttttgc
     2941 tgaggcaaaa ctgtttatca ccgcagcaac cgaaggtcgg ggaattgatg ccctgcgcga
     3001 gcatctgctt cagttaccag gacgtgaaca cgccagccaa catagtttcc gcctcgcgat
     3061 cgaccgcgca tttaccgtaa aaggtgccgg gctggtcgtc accggtacgg cgttaagcgg
     3121 ggaagtgaag gtaggcgatt cactctggct gactggtgta aataaaccga tgcgtgtacg
     3181 tgcgctgcat gcacaaaacc agccaacaga aaccgcccat gccgggcagc gtatcgcgct
     3241 taacatcgcg ggtgatgcgg aaaaagagca gattaaccgt ggcgactggc tgcttgccga
     3301 tgcgccgcca gagccgttca cacgggtgat tgtcgagctt caaacccata caccgctgac
     3361 ccagtggcag ccgctgcata ttcaccacgc cgccagccac gtcacgggac gcgtttcact
     3421 gctggaagat aaccttgctg aactggtctt cgacaccccg ttatggctgg cagataacga
     3481 ccgcctggta ttgcgcgata tctctgcccg caacacgctg gccggagcgc gcgtcgtgat
     3541 gcttaacccg ccgcgtcgcg gtaaacgtaa accagagtat ctgcaatggc tggcttccct
     3601 cgccagggca cagaatgata gcgaagcgtt agccgttcac ctgcaacgcg gcgcggttaa
     3661 tcttcctgat ttcgcctggg cgcgccagct caacggcgaa gggatgcgcg aactgctgca
     3721 acagcctggc tatattcagg ctggttatag cttgttgaat gcgccggttg ccgctcgctg
     3781 gcaacggaaa attctcgaca cattagcgac ttatcatgag caacatcgcg atgaacctgg
     3841 gcctgggcgc gaacgtctgc gacgtatggc gttgccaatg gaagatgaag cgctagtact
     3901 gttgctgatt gaaaagatgc gcgaaagcgg cgacatcctc agtcatcacg gttggctgca
     3961 tctgccggat cacaaagcgg gcttcagcga agagcagcag gccatctggc aaaaagcaga
     4021 gccgctgttt agtgacgagc cgtggtgggt gcgtgatctg gcaaaagaga cgggtaccga
     4081 cgagcaggca atgcgcttga cgctaagcca ggcggcgcag caaggaataa ttaccgcgat
     4141 cgtcaaagat cgttattacc gtaacgatcg gattgtcgaa tttgccaata tgatccgcga
     4201 tctcgatcag gagtgtggtt caacctgcgc ggcggatttc cgcgatcgct taggcgtagg
     4261 ccgaaagctg gcaattcaga ttctggaata ttttgaccgc attggcttta cgcgtcgtcg
     4321 tggaaatgat catttattgc gcgacgcatt attatttccg gaaaaataag gaaatgatta
     4381 attaattttt aaaataaatt aatacaaatt tctta
//
LOCUS       DADLFA010000235         4399 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000235 DADLFA010000000
VERSION     DADLFA010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4399
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.237"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            3..368
                     /locus_tag="JQH65_003768"
     CDS             3..368
                     /locus_tag="JQH65_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141029.1"
                     /translation="MAEWPGEGGEGHLSSFFVQTDKASINVAYHHTVNQQKELGTFGA
                     FDHDWHTLAFKFKGSNSINVTPVLDGVDGQAFDLVKWANTANGLNRFVITDITGSAET
                     YPVLIDTVEVKANKAGAAA"
     gene            508..690
                     /locus_tag="JQH65_003769"
     CDS             508..690
                     /locus_tag="JQH65_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HAY6141030.1"
                     /translation="MTFIHQVMLYFCTAVCVLYLLSGGYRAVRDFWCRQIDKRAAEKI
                     SASQSAGSKPEEPVTP"
     gene            614..961
                     /locus_tag="JQH65_003770"
     CDS             614..961
                     /locus_tag="JQH65_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HAY6141031.1"
                     /translation="MIKGPLRKSAPVSQPEANPKNPLLLNNPFQRENPMSEITSLVTA
                     EAVKEVLRSEEVRSALKQKLRQNLESRLDAEVDAILDELLGVAAVPEPEGIAGDGSAS
                     DGGEPTPDSDMMM"
     gene            1038..1253
                     /locus_tag="JQH65_003771"
     CDS             1038..1253
                     /locus_tag="JQH65_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAY6141032.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRFSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            1258..1602
                     /locus_tag="JQH65_003772"
     CDS             1258..1602
                     /locus_tag="JQH65_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HAY6141033.1"
                     /translation="MKKKYELVVKGINNYPNKITVTVALEIGGYPSLLLPDVAISLDR
                     TEDATLEFYEAEAKKQAKQFFMDVAAGLCEGDGPLPEKRPVILEAQDVLITYRGKLPG
                     IITGSLKTPPLA"
     gene            1653..2186
                     /locus_tag="JQH65_003773"
     CDS             1653..2186
                     /locus_tag="JQH65_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HAY6141034.1"
                     /translation="MNTKIKYGLSAAVLALIAAGAPAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDSKPVVPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWDIDR"
     gene            2342..2524
                     /locus_tag="JQH65_003774"
     CDS             2342..2524
                     /locus_tag="JQH65_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141035.1"
                     /translation="MAKYKRILLRLTMKNGLELKAPVTDDISRALAFAIKWVAVGVAV
                     SPMLYGLAKLVIALKS"
     gene            2682..2856
                     /locus_tag="JQH65_003775"
                     /pseudo
     CDS             2682..2856
                     /locus_tag="JQH65_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371701.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase"
     gene            3089..3313
                     /locus_tag="JQH65_003776"
     CDS             3089..3313
                     /locus_tag="JQH65_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141036.1"
                     /translation="MKKLLVTVKPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
     gene            3658..3984
                     /locus_tag="JQH65_003777"
     CDS             3658..3984
                     /locus_tag="JQH65_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HAY6141037.1"
                     /translation="MKRNLPLIILLSSLVMGCTQHKTDMPRQLVKALPQYPAYAAANY
                     IKGRVDVKFDIGADGTVTRIEFIRSEPHHLFDEQVVKAMAKWRFEKDRPCKGVKKTFI
                     FSPSAP"
     gene            complement(4116..4316)
                     /locus_tag="JQH65_003778"
     CDS             complement(4116..4316)
                     /locus_tag="JQH65_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3950 domain-containing protein"
                     /protein_id="HAY6141038.1"
                     /translation="MSSKNRTRRTTTRNIRFPNQMIEQINIALDLKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMKNNEK"
BASE COUNT         1125 a          938 c         1212 g         1124 t
ORIGIN      
        1 gaatagcgga atggccgggt gagggtggtg aaggccatct gagttctttc tttgtccaga
       61 cagataaagc cagtattaat gttgcatacc atcatacagt taatcaacaa aaagaacttg
      121 gcacgtttgg cgcattcgac catgactggc atacgcttgc atttaaattt aagggcagta
      181 acagcattaa tgttactccg gtgcttgatg gtgtggatgg acaggcgttt gacctggtga
      241 aatgggccaa tactgctaat ggactcaaca ggtttgtcat tacggatatt acaggtagtg
      301 cagaaaccta ccctgtactt attgatacgg tggaagttaa agcaaacaaa gctggagcag
      361 ccgcataatt gctaaaaaaa gccgccagcg gcaggaatgg aagctggcgg aggtaatccc
      421 aatggagaat gtaaagaaaa gatgctttcg tacattggtt ttttaaatga aaacaattct
      481 cattgtcaac cataacggta agaaattatg acatttattc atcaggtgat gctgtacttc
      541 tgtacggcgg tctgtgtgct gtatcttctt tcgggtgggt acagggctgt gcgcgatttc
      601 tggtgcaggc agattgataa aagggccgct gagaaaatca gcgccagtca gtcagccgga
      661 agcaaacccg aagaacccgt tactccttaa caaccccttt cagcgagaaa atcccatgtc
      721 agaaatcaca tccctggtca ctgcagaggc agtgaaggaa gtcctgcgct ctgaagaagt
      781 ccggagcgca ctgaaacaga aacttcgcca gaatcttgag tcgcgtcttg atgcagaagt
      841 ggatgccatt ctggatgagc tgctgggcgt agcagcggtt cctgagccgg aaggtatcgc
      901 gggtgacggg agtgcttcag atggcggtga acctacacct gacagcgaca tgatgatgta
      961 agcatgcgca agggaccatc ggtgtgtgcc ggtggtcttt atattgttgt gagcttccgg
     1021 attgcgggag acggggtatg taccagatgg aaaaaatcac aacaggtgtg tcatacacca
     1081 cgtcagcggt gggaacgggc tactggttcc tgcagttgct ggacaggttt tccccgtctc
     1141 agtgggcggc aataggcgtg ctggggagtc tgttgtttgg gctgctgaca tatctgacta
     1201 acctgtattt caaaatcaga gaggaccgtc gtaaggcggc gcggggagag taaagcgatg
     1261 aagaaaaaat acgaactggt tgttaaaggg ataaataatt acccgaataa gattactgtt
     1321 actgtggcac tggaaattgg tgggtatccg tcactgttgt tgccagatgt ggcgattagt
     1381 cttgaccgta ctgaagatgc cacgctggag ttttacgaag ctgaggcgaa aaagcaggcg
     1441 aagcagtttt tcatggatgt tgctgccggg ttatgtgaag gggatggtcc gttgccggaa
     1501 aagcgccccg taattttaga ggcgcaggat gtgttgataa cctacagagg aaaactaccg
     1561 ggaataatta ctggttctct gaagactcca ccgctggcct gaagacttaa catatccagg
     1621 gatttgaaat cgataaaccc tgataaatat ccatgaacac caaaatcaaa tacggcctgt
     1681 cggctgccgt tctggcgctg attgccgcag gtgcgcctgc gcctgaaatc ctcgaccagt
     1741 ttctggatga aaaggaaggt aaccacacca cggcataccg tgatggcgcg ggtatctgga
     1801 ccatctgccg cggtgccatc ctggtggata gtaaacctgt cgtcccgggc atgaagttgt
     1861 cgaaggaaaa atgcgaccag gtcaacgcca ttgagcgtga taaggcgctg gcatgggtgg
     1921 agcgcaatat taaagtgccg ctgaccgaac cccagaaagc gggtatcgcg tcattttgtc
     1981 cctataacat tggccccggt aagtgtttcc cgtcgacgtt ttacagacgg attaatgctg
     2041 gtgaccgcag gggagcatgc gaagcgattc gctggtggat taaggacggt ggcagagact
     2101 gccgtatccg ctcaaataat tgctacggtc aggtatcccg gcgagaccag gaaagtgcgc
     2161 tggcgtgctg ggacatcgac agataagcag aatattttgc tgaaaataag gcatgagcac
     2221 gcggacggat aacacgaaat cctgcgaact ggcgaaacgt aagtgaataa aagtaaaaac
     2281 cccgtttgtt ggcaccaggc ggggttttgt gtttctgacc ttgagtaagg caagggagaa
     2341 catggcgaag tataaacgaa ttctgttgag gttgaccatg aaaaacggcc ttgaactgaa
     2401 agcgcctgta actgatgaca tcagcagagc actggctttt gccattaagt gggtggcggt
     2461 cggtgttgct gtgtccccga tgctgtatgg gctggcaaaa ctggtcattg cgttgaaatc
     2521 gtgaagggag gattaagcat gtcagacaaa ctcataacgc tggcgaagat cctctgtgta
     2581 attgtcggca tttcattttc actaatgctg gttgctcttt ttctttccat ggcctggatg
     2641 atgttgtctt cgtcggggct gctggggtga acataaaccg aatgctttcc gcgtttaccg
     2701 ttattctgct ggtggtctgt ggtgcgctgt ggctggcaac agaccattac cgtgataacg
     2761 ccatcaccta caaagcgcag cgcgataaaa aagccagaga gctggagctg gcaaacgcaa
     2821 ccattactga catgcagcag cgccagcgtg atgttgccgc tggtcgtaag cgcctgcgga
     2881 tcaacgccac ctgctccggt accgtgcgtg aagccaccgg cacctccgga atggataatg
     2941 caaccggccc ccgactggca gacaccgctg aacgggatta tttcaccctc agagagcggt
     3001 tgatgacaat gcagaagcaa ctggaagggg cgcaggaata tatccgcact cagtgtatta
     3061 actagtattt ttgttatccg gagaatgcat gaagaaatta ctggtaaccg taaagccttt
     3121 tcagggaaca attccgttcc gtattttgca gcgtggtcgt gttcttgttg aaggttcgtt
     3181 cagtggtaaa tgtacgcaat tacactcccg gacctttcag gtgaatgcca cgaatgaaga
     3241 gctaaccgtt gagtgtacga tgaatgccgc taaatgccgc atggtatccg ctgcattaca
     3301 gccagtgtgt tgagcgacct tattaaccat gcgcggtatt gtcgccgtat ccccgcatta
     3361 acagagaccg cagcccgacc gggagactcc tctgcgcgag tgtgcgggga taatcaaaaa
     3421 cgatacacac cggggtttac cgcgttaacg gagcgcggcg ttgtcccctc atggtcgctg
     3481 gtccggtgcg atggtggaag aaaccggacg atgtgttacc tcgcaagctc tgttatgtca
     3541 tgtgtctgat ttgtgattta agtcggataa ttgtcgttgc cattaagcag aggattgatg
     3601 accgacaggg tggcattgtt agaataagac ttattcttat ctgtgccggg aatgaaaatg
     3661 aaaagaaacc ttccgttaat tattttgttg tcttctctgg ttatgggctg tacgcaacat
     3721 aaaacagata tgccccgaca gttggttaag gcattaccac aatatccggc ctatgcagcg
     3781 gcaaattata taaagggacg ggttgatgtg aagtttgata ttggtgctga tggtactgtc
     3841 acccgaattg agtttatccg ttcagagccg caccatttgt ttgatgagca ggttgtaaag
     3901 gcgatggcaa aatggcgatt tgagaaggac aggccgtgta aaggcgtgaa gaaaacgttt
     3961 atctttagtc cttctgcacc ctgattattt catcagaaat taattatcac tctgttgtta
     4021 ttctgtacat cccggcaggg taagtcttgt tccgtcggat atgaagatga aatattgttg
     4081 gaggacagtg ggtacctgct cctgtaaccg aacgttcatt tctcgttatt tttcatgctg
     4141 gccgggcgca gatgcgttgc atctgttgcc agccttctcc tgcaggcttc aataacccac
     4201 gctgaaaagt ttcctgaacc tttcagatca agagcgatgt taatttgttc aatcatctgg
     4261 tttggaaatc ggatgttgcg ggttgttgtt ctgcgggttc tgttctttga tgacataatg
     4321 tttccccata ttcagtgttg ctgatttgta ttatctgaag ttgcttttac gttaatttga
     4381 tgcagatcaa tcaatacga
//
LOCUS       DADLFA010000236         4378 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000236 DADLFA010000000
VERSION     DADLFA010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.238"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(61..723)
                     /gene="exoX"
                     /locus_tag="JQH65_003779"
     CDS             complement(61..723)
                     /gene="exoX"
                     /locus_tag="JQH65_003779"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HAY6141039.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWSFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(747..1403)
                     /locus_tag="JQH65_003780"
     CDS             complement(747..1403)
                     /locus_tag="JQH65_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HAY6141040.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLACDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            complement(1505..1735)
                     /locus_tag="JQH65_003781"
     CDS             complement(1505..1735)
                     /locus_tag="JQH65_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HAY6141041.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVKLSRLPYEPKLK"
     gene            1874..2248
                     /gene="yobA"
                     /locus_tag="JQH65_003782"
     CDS             1874..2248
                     /gene="yobA"
                     /locus_tag="JQH65_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HAY6141042.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            2252..3119
                     /gene="copD"
                     /locus_tag="JQH65_003783"
                     /pseudo
     CDS             2252..3119
                     /gene="copD"
                     /locus_tag="JQH65_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            3132..3473
                     /locus_tag="JQH65_003784"
     CDS             3132..3473
                     /locus_tag="JQH65_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HAY6141043.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(3855..4325)
                     /locus_tag="JQH65_003785"
     CDS             complement(3855..4325)
                     /locus_tag="JQH65_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY6141044.1"
                     /translation="MDLALVTGQRREDITNMRFSDIYDDRLHIRQIKTGMMIAIPLSL
                     SLPVAGLRLGAVVEQCRMVSRGDYLISAGIRKNSPDGSIHPDGLTKKFVAARKLTGIQ
                     FSENPPTFHEVRSLAGRLYKETCGEEFAQRLLGHTSEKTTKMYLDEREKTYLLL"
BASE COUNT         1089 a         1056 c         1080 g         1153 t
ORIGIN      
        1 gatggaatat gcttttctgg tcgcgctggc gcagggaaca ttacccgcta cgcctgcgga
       61 ttaagtattt tccagataat gtttcagtgt taaacgcagc tccgggctca tgctgtccag
      121 gttattaaat gaccagcgca gatagcccgg atcgcgttcg gcaacgtcgg aaaccgcttt
      181 gccacggtat ttgccaaagg tgaaggtcgt catcaacgac ggacgtccgg tgatatcggc
      241 catctgttct gccgtccagc cggaggtgtt cataatatcg ataagcaacg cggcggtgat
      301 ataacagtca tacaacgcgc ggtgatgatg caggcccggc ggcgtctgta cattgagctt
      361 ccgtgtttta tataacgcca tattgctgta cttgatccct ggccacaaac gacgggccag
      421 tttcatagtg caaatccact caccgggcat ctcaggcagt acgcggcggt caaagctggc
      481 gttgtgcgcg acataccatt cactaccgta atagtgtggg atcacatctt caatccacgg
      541 tttatcggcg accatggctt cggtgatgcg atgaatcgcc atcgcttgtg gactaatagg
      601 acgatcgggg cgcaccaggt ggctcatggg gttgacgatt tttccgtcaa tgacatcaac
      661 agaggcaatc tcaacgatcc ctccctgcaa accgcaggtt tctgtatcga taatgcgcaa
      721 catggcatgc tccaggccga aaaagcctag cgtaatggaa tgatatcgcc ttgccaaccc
      781 tgtgatgaac gctgaccgac taacaataat gaaccacgat cggcgcgaac gatgagacga
      841 ccatattcat cccatagtgc actgttacca cgggcatttg ccatcagcac ggcaatcgag
      901 tactgatgtg aaaaaaattg taagcgacgg gccgacgcca gcaggtcggg ttctcccaga
      961 cattgccctg tagtaaacag tgaacaggtc gggtccatat ccataccttg cggttgttca
     1021 tcgaccaccg tgatggttct ggaacgacgg cccagacatg caccatgact ctgatggtag
     1081 atcccaggcg tttttcgcca cggtgcgaac accgcaattc cacgaataaa gcgatcgtta
     1141 tattcaacgg gaaggccggc aatgatggtc attcgccagg tcgttgcggc ataacaaagc
     1201 gggtctaaca gtgagagatc gggtggggca ggaagggcac gtcgcgaata atcacacgcc
     1261 agtaaagaaa gcgatggaaa aactaacagc tgacattgtt gcctggcggc ggccctcaca
     1321 aactccagat gatgagctac ctgttcggtg agtgaggttt tacgaggctc atattgcgct
     1381 gctgcaactt tccagaacga cataatgact tccttttcaa aatgactgca aattatgggt
     1441 gcggtttaga aaatactcat aatttagcag gcgttatgta agaaagtgta actctgtata
     1501 agttttattt aagtttgggc tcgtaaggta aacgtgacag tttgaccgaa gccaaacggt
     1561 gggcaataag cctctcgcga aaccagctgc gcaaatgttc aggctgttca cgttcaaccg
     1621 cttcagcgat caccggcata ttgtagcgtt ctttaaatgc caccccggcc gccgccaaat
     1681 cgacattcac tttatccatt tctgtttgat ccagtttagc cagattcttc agcattctta
     1741 atctcctcat cattcgcggg caaaagttac taagagtgag ccaggatggc aagccggaat
     1801 tgatgatcct cgactcagac attagacaag gttatgctct gtaaaaaagt tattacaaaa
     1861 agtaatagat aatatggctt caactgcacg ctcccttcgc tacgcgctgg caatcctgac
     1921 cacttcgttg gtgacacctt ccgtctgggc acatgcgcat ttaacgcatc agtatcctgc
     1981 ggcaaacgcg caagtgacag ctgcaccgca ggcaatcact ttaaacttct cggaaggtgt
     2041 tgaaacagga ttcagtggtg caaaaatcac ggggccaaaa aacgaaaata ttaaaacatt
     2101 accggcgaag cgaaatgagc aggaccaaaa gcaactgatt gttccactgg ccgattcgct
     2161 gaaacccgga acctacaccg ttgactggca tgttgtttcg gtggacgggc ataaaacgaa
     2221 aggacactac acctttagcg tgaaataaat catgctggcg tttacctgga tcgcgctgcg
     2281 atttatccat ttcacctcgc tgatgctggt ttttggcttc gcaatgtacg gtgcctggct
     2341 ggcccccttg acgattcgtc gcttgctggc gaagcgtttt ctgcgtttac aacagcacgc
     2401 tgccgtctgg agtttgatca gtgccactgc aatgctcgcc gttcagggcg ggttgatggg
     2461 aacgggatgg acagatgtat tttcgccaaa catttggcag gctgttttgc aaacgcagtt
     2521 tggtggcgtc tggttatggc aaattgttct cgccctcgta acgttgcgcc cttatgcaac
     2581 cgcggaatat gccacgtttg ctgtttatgc tcactaccgc gcaatttatt ctgctggcgg
     2641 gagtggggca tgcgacgctg aatgaagggg taacggcgaa aatccatcag actaatcaca
     2701 cgattcacct gatttgtgcg gcggcgtggt ttgggggatt actgccggtg ctctggtgta
     2761 tgcaactcat caaaggccgt tggcgacacc aggctattca ggcgctgatg cgtttttcat
     2821 ggtgcgggca ttttgcggtg atcggcgtac tggcaagcgg ggtgcttagt gcattgttga
     2881 ttaccggatt tccccccacg cttacgactt actggggcca actcttattg ctcaaagcca
     2941 tcctggtgat gattatggtg gtcattgcgc tggctaatcg ttatgttctt gtaccgcgca
     3001 tgaggcagga tgaagatcgt gcggcaccct ggtttgtgtg gatgactaaa ctcgaatggg
     3061 cgataggtgc ggtagtgctg gtgatcatca gcctgcttgc gactctcgaa cctttttgat
     3121 ggattacgaa aatgatgaaa aaaagtattc tggcgtttct gttactcacc agttctgcag
     3181 cggcgctggc tgcaccgcag gtgattaccg tcagtcgttt tgaagtgggt aaagacaaat
     3241 gggcgtttaa tcgcgaagag gtgatgctga cttgccgacc gggtaatgct ttgtatgtta
     3301 ttaatccgag taccctcgtg cagtatcctt taaacgatat cgcacaaaag gaagttgcca
     3361 gtgggaagac caatgcccaa cccatttcgg tgattcagat tgatgatcct aacaatcccg
     3421 gcgaaaaaat gagtctggca ccgtttatag aacgagctga aaaactctgt taattaccta
     3481 aaatagcctt ttgatttcca ataaaaaaac cgcctcagtt ctttcaccag aacgggcggt
     3541 tttaacattt cagctgatga ccaccacgct ttttattgac cattttgcac gcaaactgga
     3601 aaacctggcg tcgtcatcta ttcttaaagg gcaaggcaac taagcctgca ttaatgccaa
     3661 cttttagcgc acggctctct cccaagagcc atttccctgg accgaataca ggaatcgtat
     3721 tcggtctttt tttaattgca tttgaaatca attagttgca aatgtctccc cgaaatttac
     3781 tcgaatttct gtattccggt cttttttggt tatatcacaa ccaaaataca tttaacaatc
     3841 catttacgtt aaaatcagag cagtaagtac gttttttctc tctcatcaag atacattttt
     3901 gttgtcttct ccgatgtgtg gccaagtaga cgctgagcaa attcttctcc acatgtttct
     3961 ttgtacaatc gtccagccag gcttctgact tcgtgaaaag ttggtgggtt ttcactgaac
     4021 tggatacctg ttaattttct ggctgcgaca aatttctttg tcaggccgtc cgggtgaatg
     4081 ctgccgtcag ggctgttttt tctaatcccg gcactgatta gataatcccc cctacttacc
     4141 atgcggcact gttcaactac tgcaccaagt cgtaaaccag cgacaggaag gctgagtgac
     4201 agggggatag caatcatcat tcctgtctta atttgcctga tgtggagacg atcatcataa
     4261 atatcactga accgcatatt cgttatgtct tcgcgacgtt gtcctgttac aagggctaaa
     4321 tccatagcta atgggaacca tgccggaagt tgatctgctg cctccctgat gcagttgt
//
LOCUS       DADLFA010000237         4373 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000237 DADLFA010000000
VERSION     DADLFA010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4373
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.239"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..100)
                     /locus_tag="JQH65_003786"
     CDS             complement(<1..100)
                     /locus_tag="JQH65_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HAY6141045.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVE"
     gene            complement(113..1894)
                     /gene="gcl"
                     /locus_tag="JQH65_003787"
     CDS             complement(113..1894)
                     /gene="gcl"
                     /locus_tag="JQH65_003787"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HAY6141046.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTYFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAVDAPTETCFMHYE"
     gene            complement(1984..2799)
                     /gene="allR"
                     /locus_tag="JQH65_003788"
     CDS             complement(1984..2799)
                     /gene="allR"
                     /locus_tag="JQH65_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HAY6141047.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(2877..3359)
                     /gene="allA"
                     /locus_tag="JQH65_003789"
     CDS             complement(2877..3359)
                     /gene="allA"
                     /locus_tag="JQH65_003789"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HAY6141048.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            3589..>4373
                     /gene="allS"
                     /locus_tag="JQH65_003790"
     CDS             3589..>4373
                     /gene="allS"
                     /locus_tag="JQH65_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HAY6141049.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSKLQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPT"
BASE COUNT         1117 a         1185 c         1044 g         1027 t
ORIGIN      
        1 attcaacgcc gcgaaaacca cactgcgcag ctttctcaaa acgggcgaga aaatcatatt
       61 caccaaataa catcgataaa ttagcagaga aacgtaacat tatttatctc ccttattcat
      121 agtgcatgaa gcaggtttca gtcggtgcgt ccactgcgtt atcggcgata tcttcaaatt
      181 ccatgacgtt atccagttcg ctgcccatcg aaatattggt cacacgctcg agaataactt
      241 ccacgactac cggtacccga tattgcgcca ttaaggcttt cgcctgttca aaggctggcg
      301 caatatcttc cggtttgaag acgcgaatcg ctttacaacc taaaccttcc gctactttta
      361 cgtggtcgac gccgtaaccg ttcacttcgc tggagttgat attctcgaaa gcgagttgca
      421 cgcagtagtc catgtcaaag gcgcgttgtg actgacgaat caggccgaga taggcgttat
      481 tcaccagcac atggatgtac ggaatgttga actgcgcgcc aacggctaac tcttcaatca
      541 ggaactggaa gtcaaagtcg ccagaaatcg ccaccacatt gcgtttcgga tcagcggcac
      601 aaacccctag cgcagccgga atcgtccagc ctaacggacc agcctgacca cagttgatcc
      661 agtggcggtc tttaaagaca tgcagcattt gtgccgcagc gatttgtgac agaccgatgg
      721 tggtgacata acaaacatcg cgaccaaagg ctttgttcat ctcttcatac acgcgctgcg
      781 gtttcaccgg cacgttgtcg aagtaggttt tgcgcagcaa agtgcgtttg cgctgctggc
      841 agtcggcgac ccattcttta cgacacggca gacgacccgc tttttgcatc tcctgcgcca
      901 cttcaaccag cagtgtcagc gccgctttag catcagagac aatgccgaga tccggacaca
      961 gcacgcgacc aatttgcgtc ggctcaatat cgatatgaac gattttgcgc ccttcggtgt
     1021 atttctctac cgaaccggta tgacggttag caaaacggtt accgatacca aacaccatgt
     1081 cggacgccag caacgttgcg ttaccgtaac gatgcgcggt ttgcagaccg accatcccgg
     1141 ccatcagttc atgatcgtcc gggatacagc cccagcccat cagcgttggg atcaccggaa
     1201 cgctggtcag ttcagcaaac tgttgtaaca gtgcagctgc gtcagcattg attaccccgc
     1261 ccccggcaac aatcaccgga cgttcggcct ggattaacat ttctacagct ttttcgatct
     1321 gcatacggct ggcagcaggt ttgtagaccg gcagcggttc gtacatgtca ggatcaaact
     1381 cgatttccgc aacctgaacg tcgaacggta aatccaccag taccggaccc ggacgaccag
     1441 aacgcatcag gtgaaatgcc tgttgcagca cgcgaggcac cagcgccgct tcacgaactg
     1501 taaccgccat tttgctgacc ggtttagcaa ttgcttcaat atctacggcc tgaaaatctt
     1561 ctttatgcag acgggcgcgc ggtgcctggc cggtaatgca cagaatagga atggaatcag
     1621 cagaagcgga atagagcgcg gtgatcatgt ccgtgcccgc aggaccggaa gtccccagac
     1681 atacgccgat attccctgcc gttgcgcggg tataaccttc cgccatgtgc gaagcacctt
     1741 ccacatgacg cgccagaatg tgacgaatac cgccgtgctt acgcatcgct gagtagaacg
     1801 gattgattgc agctcccgga acaccgaagg cggtggtgat accttctttc tccagcacat
     1861 acattgccgc gtcaacggct ctcatttttg ccattttatt cctatctcta tttattggaa
     1921 aatttttcca actttcaaat tttgtcaaaa aaacccgctc cgcagagcgg gatgcgacag
     1981 acattatgga tgtgctttca gtcccaacgc cgtgctgata tcgcgggcgg tgtctctgac
     2041 cagctcaccc tgactgacaa aacgatcttc tgtcagtctt gatgaaggcc cggagatgga
     2101 gatagcggca acaacgctac cgacatcatc gtaaattgct gaagctatgc aattcagacc
     2161 tacaacatgc tcttctttat ctacggtata gcccagttca cgcgcttgtt ccaggtcctt
     2221 cagcaaggtg ggcatatcca caagcgtagt tggcgtaaac tgctgcaaac cggtttgcag
     2281 aatgatgctc atcaactcct cttccgccag cggataaagc agcgctttgc ccgcaccgga
     2341 agcatgcagc ggcagacgac tgcccagtgg cgcacacatc ctgaccattg atttacactc
     2401 taactgacca attaatactg cttcattgcc gttacggatc gcgacattga ccgtttcgcc
     2461 ggaaagtaac atcaggcggc gcataaacgg cccggcgacg gagaggacat cgcggttatg
     2521 gatgtacgcc gcaccgacgt taaagacacc taatcctata tgccaccagc ctaattgact
     2581 gtcctgatag acaaaatccg ctgcttgtaa aaccttcagc aagcgaaagg tcgtggagag
     2641 cggcaaatcc agattgagag aaatatcgct aaccgacgaa cttcccccac ttttttccag
     2701 atattgcaga atcgcaattc cccgctctaa cgcctgtgcg cccttctgtg ccacaggctc
     2761 cgcctgtcct ggcctgccgc gccgtctaac ttccgtcata cctacgttaa ctccgaccta
     2821 caagtgagca taaacaatga acaattagtt tatcttatgc aagtcggttg caggcgtcac
     2881 gcaaaacaga gttcctgttc aggaatactt tcaacatcac agttgtcact gccgccgcga
     2941 tcgatggtca gaaaatcggt gacgcgctgc caggcgaaaa gtgggtgatg ccagacgtta
     3001 cgatggtaat tcactccctg ttcgccgttg gtgataaacg cccgcagcgt tgacaggtct
     3061 ggtttgtcgt cgcctaacgc cacgaccacc acaaacactt cacctttcat cgggataaag
     3121 gcctgagtgc ccagcggatg acgttcgagt tcgtgaatgg tcagcggcag attcgccggt
     3181 tgcgcgcggt taatgctaat aagcgtacgg tcttgctcaa gaatctcaac cagcgccaaa
     3241 tcgtggtaac gctccaccag gccattgtta atatggaaaa aatcccgttg ctgagtttcg
     3301 attacgtcgc cataagcact aaaggcttcc tgacttaacg gtaatacctg aagtttcatc
     3361 tttttgtcct tgtaacataa ttccgttcaa ccaccgccga ccggtcagct tgggttcaat
     3421 aacaggcaat ctaaaacctt aagagaacaa catcaataca aaagttggaa tatttttcca
     3481 aaactagatt tagtccacaa attgaacagg gttatcatgt aaaaatactg ataaatcaga
     3541 ggataagaaa atcacgcaac ttgcggattt aaaaggggat acctgaagat gttcgatcca
     3601 gaaaccttgc ggactttcat tgcggttgct gaaacaggaa gtttttcaaa agcggcagaa
     3661 cgattatgta aaaccacggc gacgatcagt tatcgcatta aacttctgga agagaatacc
     3721 ggagtagcgc tgtttttccg tacgactcgt agcgtaacgt tgacagcggc tggcgagcat
     3781 ctactttccc aggccagaga ctggctgagc tggctggaaa gtatgcccag caagctgcaa
     3841 caggtgaatg atggcgtgga acgccaggtg aatattgtca tcaacaacct gctctataac
     3901 ccccaggccg tcgcccagtt gctggcgtgg ctgaatgaac gttacccctt tacccagttt
     3961 cacatctccc gacaaatcta tatgggcgtc tgggactcgc tattgtacga aggtttttcg
     4021 ctggctatcg gcgtcacggg aactgaggcg ctggcaaata cctttagtct tgatccctta
     4081 ggatcggtgc aatggcgatt tgtcatggcg gcggatcatc cgctggcgaa cgttgaagag
     4141 ccgctaacag aagcgcagtt gcgtcgcttt ccggcggtca atattgaaga cagcgcccgc
     4201 accttaacca aacgcgtcgc ctggcgattg ccagggcaaa aagagattat tgttcctgat
     4261 atggaaacga aaatcgccgc ccatctggcg ggcgttggca ttggtttttt gccaaaatcg
     4321 ctttgccagt caatgatcga taatcaacaa ctggtcagcc gggtaatccc aac
//
LOCUS       DADLFA010000238         4320 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000238 DADLFA010000000
VERSION     DADLFA010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4320
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.240"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..976)
                     /locus_tag="JQH65_003791"
     CDS             complement(<1..976)
                     /locus_tag="JQH65_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HAY6141050.1"
                     /translation="MIRKSATGVIVALAVIWGGGIWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSHFQMQISFDNGAPDLNIKPGQKVAFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLTRLE
                     EKKFPVGESESKFNQVNIINQGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEV"
     gene            complement(1131..1757)
                     /gene="dsbA"
                     /locus_tag="JQH65_003792"
     CDS             complement(1131..1757)
                     /gene="dsbA"
                     /locus_tag="JQH65_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HAY6141051.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(1774..2760)
                     /gene="srkA"
                     /locus_tag="JQH65_003793"
     CDS             complement(1774..2760)
                     /gene="srkA"
                     /locus_tag="JQH65_003793"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HAY6141052.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRIVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDSTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(2837..3106)
                     /locus_tag="JQH65_003794"
     CDS             complement(2837..3106)
                     /locus_tag="JQH65_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HAY6141053.1"
                     /translation="MKCKRLNEVIEFLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            3176..3760
                     /gene="mobA"
                     /locus_tag="JQH65_003795"
     CDS             3176..3760
                     /gene="mobA"
                     /locus_tag="JQH65_003795"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HAY6141054.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPPDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            3742..4269
                     /gene="mobB"
                     /locus_tag="JQH65_003796"
     CDS             3742..4269
                     /gene="mobB"
                     /locus_tag="JQH65_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HAY6141055.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGARHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         1169 a         1021 c          966 g         1164 t
ORIGIN      
        1 caacttcatc gtttgctaac tcagggtgtg cagcaagctg cttctgcatc gcaatgttat
       61 actggatgat aaattggcgc actgctgagg gatcaataga ttcagcaatc aatgaccact
      121 caccactacc gagttgctga ttcccttttg ttaacttatc aagttcgtag gtcagattga
      181 cattgatata atctttgtct ttaacgcgat ccagcctggt ttttgcaacg aaagcatcga
      241 tttgggcaac gtcttccccc tgattgataa tattaacctg attaaatttc gactcgcttt
      301 caccaaccgg aaatttcttt tcttctaatc gtgtcagaga atcaatggta aaactttttg
      361 ccgttacttt gttcattggt gataattgca gaacaatatc ttcgactttg ccttcaatat
      421 ccaggttaga caatgaacta ccatcaccat taaaagtaaa ctggccctca ccaaaagaaa
      481 actttccata ctctgcaggg gcaacatcta atgtcgtaga gaatgagcca ccaaacgcga
      541 atcgcaatgt cgcttccacg ggcgatttat ttttcgcggc gataaatagc ggttgtgtca
      601 gttcattatt cactaagttc acttttgccg ctgccagtgc tgggatgaca ttaccatgca
      661 ttaacattgt gatgggcaac ggaccgtgct caacatccac atcaaatgca actttctggc
      721 ctggcttgat attgagatcg ggtgcaccgt tatcaaatga aatttgcatt tgaaaatggg
      781 aattaaaaaa gcctttgtca aagtttttat aacttaacgt catatcgtag gcatgttcac
      841 ctttcttttt agcatcgtta aaatctttaa taaatttttc gacaccaggc tgaatttgcg
      901 tacctgtgta ccatatgcca ccaccccaga ttacggctaa cgcaacaata acacctgtag
      961 ctgacttacg tatcatataa atattccctg ataaaacagc aaaactttga atatccactt
     1021 atgctgaaat aatgtccagc ggcaggatgt attatcagaa tgtatttata tttattaaat
     1081 aataaaaaaa gcccgtgaat attcacgggc tttatgtaat ttacattgaa ttattttttc
     1141 tcggacagat atttcactgt atcagcatac tgctgaacaa aaacatccat attgctggta
     1201 tccataccct gcggattcag ctgatattta ccgttaacaa acatcgccgg aacaccgcgc
     1261 aattgcacgt cagctgcagc tttttcctgc tgagctacca gagatttcac cacgaagctg
     1321 ttccacgccg cgtcgtactc ttcaccttta atacctgcgt tgataaatac atcgcggata
     1381 tcagaagcag aacgaatggt ctgggttttc tgtacgcctt caaacagcgg aacagtcact
     1441 ttgtcttcca cgcccagcgc catcgccaca gcccatgcct gagtcagatc tttgcccagg
     1501 tcaccaccca tgaagttgac gtggtattta gtcatcttca cgccttccgg cagttttttc
     1561 ttcacattat cagaaatatg cagaacttct tcaaactgat agcagtgcgg acagaagaaa
     1621 gagaaaaact ccagcacttg cggcgcgcca gctaccggtt tttccagggt agtgtactgt
     1681 ttaccatctt catactgcgc cgccgatgcg ctaaacgcta aaactaaacc agccagcgcc
     1741 agccaaatct ttttcatgat ctactctctc cgattaatac ataggtgtta attgtaaagg
     1801 gggttcttgt agaacttttg cctgttctat aaaagtcgcc gtttgtcgca gccagtaatc
     1861 ttccccggtt aaccacggga aatttttcgg gaacgcggga tcagcccaac gccgcattag
     1921 ccaggcaaga taataaacca aacgcatggc gcgtaaaggt tcaatcagtc cgatttcagc
     1981 ggtgtcgaac tcgctaaatt cttcataagc ttcaataata gtttccagtt gcatccgctg
     2041 ctcggcttta tcgccattga gcaacatcca caaatcctga atagctggac cattacgtgc
     2101 atcatccaga tcaacaaaca ttggaccatc gcgccagaga atattcccgg cgtggcagtc
     2161 tccatgtaag cgcagaacgg tggaatcttc ccgccagtgc gctgtaacgg cggcgatcag
     2221 ctcatctgtc gctttcagga atgccgcttt caacccggaa ggtatcagtg tagcgtcctc
     2281 aaacagcttg cgtggctcaa tgagatattc attcagaccg atggtcgggc gatggataaa
     2341 aagctgtttg cgccccgtct ggtgcatacg ccctaaataa cgcccaaccg cttccatctg
     2401 atcgatatta tcagcttcga actggcgacc accgacgctt ggaaaaacag cgaaataaaa
     2461 tccctgatga ttcaataaag tctgaccgtt aaaggccaca ggtgctgcga ccggaacttc
     2521 atcatttacc aactgcaacg caaattgatg ttcttcgagg atttgatcgg ctgtccaacg
     2581 ttcagggcga taaaatttga cgacaatacg tcgacgatct tcgtcctgaa attgatagac
     2641 acggttttca tagctgttaa gcggggtaag accggaatcc acccggatcc cttgctcaaa
     2701 tagagcatcc atgatggtat ccgggtgtag tgtctggaaa gtaaaagcgc tgttgttcat
     2761 cccatcatcc ggaaaacatt acgaatgatg aaggatatca tttcacgatg attttggtgg
     2821 cgccgcttac aagcttttac tctttaatta cgccgcgagc gcgtaacagc gccgttttga
     2881 agtcttcttc ataatctttc tgcaaacccg ggatcaccgc atctttcgcg gaatcacgca
     2941 ttttcaggtg atagatcaga atgtcatccg tcaaatccgc cagttcgccg tcaaaacctg
     3001 actctttcgc cagtttctgc aaaaattgca acaggttaag gtctggctct ttttgccagg
     3061 ctggctggag gaattcaata acttcattca gacgtttaca tttcatagtg atgctcctta
     3121 ctcttgagac agacacgtta gcagggtcaa tcccacaata aaagaggcga tatcggtgaa
     3181 tctgatgacg acgataacag gcgttgtgct ggcaggcggt aaagccagac gaatgggtgg
     3241 cgtagataaa ggattgcttg aattaaacgg caaaccatta tggcaacatg tcgctgacgc
     3301 gcttatgacg cagctctctc acgtcgtggt taatgccaat cgtcatcagg aaatctatca
     3361 ggcaagcggt ctgaaagtga ttgaagattc actggcggat tacccaggcc ctctggcagg
     3421 aatgctttca gtaatgcagc aggaagctgg tgagtggttt ttgttttgcc cgtgcgatac
     3481 gccttacatt ccccctgatt tagcagcccg gcttaatcat cagcgcaaag atgcgcctgt
     3541 cgtgtgggtc catgacggtg aacgcgatca cccgactatt gctctggtaa accgcgctat
     3601 tgagccttta ttactggaat atctgcaagc aggagaacgc cgggtaatgg catttatgcg
     3661 tctggctggc ggtcatgcgg ttgatttcag cgatcataaa gatgcatttg ttaacgtgaa
     3721 tacgccagag gagcttgccc gatggcagga aaaacgatga taccgttact cgcctttgcc
     3781 gcgtggagtg gtaccggaaa aactacgctt ttgaaaaaac tgatcccggc attatgcgcc
     3841 agagggatcc gtccagggct gattaagcat acgcaccatg atatggatgt tgataagcca
     3901 ggcaaagata gctatgagct gcgcaaggct ggcgcggcac aaaccatcgt tgccagccag
     3961 caacgatggg ccttgatgac ggaaacacca gacgaagaag agctggatct acagtttctc
     4021 gcaagtcgaa tggatacctc aaagctggat ttgattctgg tcgaagggtt taagcatgaa
     4081 gagatcgcaa agattgtgct gtttcgcgat ggagccagac atcgacctga agaattagtg
     4141 atagacaggc atgttattgc tgtagccagt gatgtgccgc ttaatcttga tgtcgcatta
     4201 ctggatatta atgatgttga ggggctggct gattttgtgg tggagtggat gcaaaagcag
     4261 aacggataag caagagtagg ctggatcaga cgcagccagt gtcgcatccg acctttttct
//
LOCUS       DADLFA010000239         4305 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000239 DADLFA010000000
VERSION     DADLFA010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4305
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.241"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..590)
                     /locus_tag="JQH65_003797"
     CDS             complement(<1..590)
                     /locus_tag="JQH65_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY6141056.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEA"
     gene            complement(801..2198)
                     /gene="ccp"
                     /locus_tag="JQH65_003798"
     CDS             complement(801..2198)
                     /gene="ccp"
                     /locus_tag="JQH65_003798"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HAY6141057.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            2601..4250
                     /gene="treF"
                     /locus_tag="JQH65_003799"
     CDS             2601..4250
                     /gene="treF"
                     /locus_tag="JQH65_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreF"
                     /protein_id="HAY6141058.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVKRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT         1101 a         1098 c         1015 g         1091 t
ORIGIN      
        1 gcttcaatca tgcgtttcgg gtccataaac aactgaccgg ggcgcatagg gatctcacgc
       61 agctccacat cccagtagcg ggcgaattta tgccagcaga tttgtaccgg accgcacacc
      121 aggtttggtt tatccgttgg tttgcctgca gcttccatac gcttgcgcca acgccatttc
      181 atcgccatcc cgccgagcat acaggcctcg gaagaaccaa tggtgttggt gccaacggcc
      241 tgaccatttt tcggcgcagg cgcatgccac agatcggcaa ccatatttac gcaacgcagg
      301 tcgatggctg cggattgcgg atattcttct ttgtcgatcc agtttttatt gatcgacaaa
      361 tccatcaatt tatggacgtt ttcgtcgtcc caggtctggc agaaagtggc caggttctga
      421 cgagcgttgc catcaagata taattcatca ttgataatct gaaatgcgac atcatcgcgc
      481 atttcgtgca gcggaaatcg ttttgactcc gcgatagtag aaatggcctt tgcgccaaaa
      541 cgtgaatcga gtagttctga gcggaaatcc gttaacagct tctggtccat ttcgaactcc
      601 ttaaatttat ttgaaggcaa taaaaaagta ggatttatcc gcaatggaag caaggcatta
      661 caggcttaat ttaaataaca aaatcctaag cagaaaaacg actttttata atataaacat
      721 ttaacatgat aatattaaaa acagacttta ttatcgtaat aaaaatcgcc caaacattcc
      781 tgttcgggcg atcaaattag ttattgttta tcctgcatat acggcgtgta caccccgttc
      841 agactgtgca ggaaagctac gatatcatcc acatcctcct gcggcagctc tttgcctacc
      901 tgatagcgca gcatcagttt caccgccccg tccagcgtcg gcacgtcacc gcgatggaag
      961 tacggtgccg ttaaagcaac gttacgtaaa ccgggtactt tctggcgcaa tttatcacgc
     1021 tcttctttag tcacattcat acgaccaata tccgccgccg taatttcccc aaagttaaag
     1081 tcttttttca gccccaacgg ttcaaaggaa cgtccgccga gaataatacc accatgacaa
     1141 gttgcacatt tattatcttt aaataattga tagccttttt tctgttgcgc cgtcagcgca
     1201 ttttcatctc cacgcaacca tttatcaaat ggggaatccg gcgtaattaa tgttttctca
     1261 aattcagcaa tggcatcagt aatattttcg ccactgaaac cttgcggata aacttcgagg
     1321 aactgcgctt taagctgcgg atctttttcc agcttagcaa taatttcgtc ccaggatttc
     1381 gacgccattt caatcgggtt caacggcggt ccacctgcct gatcctgcaa tgttgccgca
     1441 cgaccatccc agaactgctc aacgttaaat actgagttaa ataccgtcgg cgcgttaatc
     1501 ggcccaactg cgccaccaac accaatcgat gtttttctgc catcgacgcc ccccgcattc
     1561 aacgcatggc aatgagcgca tgaaatggtg ctatcagccg ataaacgggg atcgtgatac
     1621 agcgcaaagc ccaacgccac tttttgcgca tcggtaggca gtttttgcgg gatgggctgc
     1681 accggttcat tgcgatgctc cggagcagta tcattgctgg cgtaatattc cgcgcgctgt
     1741 tttgcaatcc aggccagtat ttccgcccgc tcttcatcac tcaccttacc cgcccagtgt
     1801 agcgcggtat aacgcgttgg tggcatagtt tcatactgca tcacccattc aatcttattc
     1861 agatcgcttt gcgaaacggg tttatcagcc agcagtgccg cacgcacggc ctcgaggtta
     1921 aaagatttat atccaagctt aatgtcgtaa tccatcaact gtttcgcgcc aggaatataa
     1981 taataggcgg gtaattctgc cgaaggcgtg tggcaatagt cgcatccttt ttcgcggaga
     2041 aagcctaaaa ccttattatt ttcactgaca gcagatgcct gaacatcggc ctgtttactg
     2101 cgtttattat cgtggtacca cacataacca gataacccta aatagcaaat cgcgacgcca
     2161 gccaggccga tcgcggtaat acgtgagacc attttcatta ttcttccctc acggtggtga
     2221 gttattgtga cccagaaaat tttccagaat gctgcaaacg tctctgcgag acaaagccat
     2281 cataaaaaac agggagatac gcttttgata ggaatcaatc aattacatga atataaacta
     2341 tcaataagat agcctgtgcc tattacccag atcaggaatg gcatatcatg attgcggcga
     2401 gagcaggatt gaatgttctg aaaatgaaaa acaacaccaa atccgtattt ttataaattc
     2461 cattgatatt agtgcgttat attttttgac aagttaataa acgtgatcca ccgcacgctt
     2521 tgtcgcccac caggcggagc gaatgactac ccttaaagaa aagccgataa ttagcgacga
     2581 atttcggagg ttggatcctt atgctcaatc agaaaattca aaaccctaat ccagacgaac
     2641 tgatgatcga agtcgatctc tgctatgaac tggacccgta tgaattaaaa ctggatgaga
     2701 tgatcgaggc agaaccggaa cccgagatga ttgaagggct gcccgcctct gatgcgctga
     2761 cgcctgccga tcgctatctc gaactgttcg agcatgttca gtcggcgaaa attttccccg
     2821 acagtaaaac ctttcccgac tgcgcaccca aaatggaccc gctggatatt ttaatccgct
     2881 accgtaaagt gcgccgtcat cgtgattttg acttgcgcaa gtttgttgaa aatcacttct
     2941 ggctgccgga ggtctactcc agcgagtatg tatcggaccc gcaaaattcc ctgaaagagc
     3001 atatcgacca gctgtggccg gtgctaaccc gcgaaccaca ggatcacatt ccgtggtctt
     3061 ctctgctggc gctaccgcag tcatatattg tcccgggcgg ccgttttagc gaaacctact
     3121 attgggattc ctatttcacc atgctggggc tggcggaaag tggtcgggaa gatttgctga
     3181 aatgcatggc cgataacttc gcctggatga tcgaaaacta cggtcacatc cccaacggca
     3241 accgcaccta ttatttgagc cgctcgcaac caccggtttt tgcgctgatg gtggagttgt
     3301 ttgaagaaga tggtgtacgc ggtgcgcgcc gctatctcga ccacctgaaa atggaatatg
     3361 ccttctggat ggacggtgca gaatcgttga tccctaatca ggcctatcgc catgttgtgc
     3421 ggatgccgga cggatcgctg ctcaaccgtt actgggacga tcgcgacacg ccgcgtgacg
     3481 aatcctggct tgaggacgtt gaaaccgcga aacattctgg tcgcccgccc aacgaggtgt
     3541 accgcgattt acgagcgggg gcggcctccg gttgggatta ctcttcccgt tggctgcgtg
     3601 atactggtcg tctggcgagt attcgtacca cccagttcat ccccatcgat ctgaatgcct
     3661 tcctgtttaa actggagagc gccatcgcca acatctcggc gctgaaaggc gagaaagaga
     3721 cagaagcact gttccgccag aaggccagtg cccgtcgcga tgcggtaaag cgttacctct
     3781 gggatgatga aaacggcatc taccgcgatt acgactggcg acgcgaacaa ctggcgctgt
     3841 tttccgctgc cgccattgtg ccactctatg tcggtatggc gaaccatgaa caggccgatc
     3901 gtctggcaaa tgccgtgcgc agtcggttac tgacacctgg cgggattctg gcaagcgagt
     3961 acgaaaccgg tgaacagtgg gataaaccca acggctgggc accgttacaa tggatggcga
     4021 ttcagggatt taaaatgtac ggcgatgacc ttctgggtga tgaaatcgcg cgaagctggc
     4081 tgaagacggt gaatcagttc tatctggaac agcacaaact gatcgaaaaa taccatattg
     4141 ccgatggtgt tccacgcgaa ggcggcggtg gcgagtatcc gttgcaggat gggtttggct
     4201 ggactaacgg tgtggtacgc cgtttaattg gtttgtacgg cgaaccataa tatttttaca
     4261 gccagccgct aacttcctgc tggctgtaaa attatcctct tcagg
//
LOCUS       DADLFA010000240         4294 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000240 DADLFA010000000
VERSION     DADLFA010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.242"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            46..2208
                     /gene="ybiO"
                     /locus_tag="JQH65_003800"
     CDS             46..2208
                     /gene="ybiO"
                     /locus_tag="JQH65_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HAY6141059.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIHLCAL
                     PLYRKMGLWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIHDETARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLVGVRAPVQTY"
     gene            complement(2268..3194)
                     /gene="rlmF"
                     /locus_tag="JQH65_003801"
     CDS             complement(2268..3194)
                     /gene="rlmF"
                     /locus_tag="JQH65_003801"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HAY6141060.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            3470..3729
                     /locus_tag="JQH65_003802"
                     /pseudo
     CDS             3470..3729
                     /locus_tag="JQH65_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
     gene            3994..>4294
                     /locus_tag="JQH65_003803"
     CDS             3994..>4294
                     /locus_tag="JQH65_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HAY6141061.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPG"
BASE COUNT          998 a         1129 c         1076 g         1091 t
ORIGIN      
        1 gcatctatac ttatcttttt gctctgtttt ctcactggag gagtcatgcg gtggatcctg
       61 ttcatcctct tctgcctgct gggcgcacct gcccacgcgg tatccatacc cggcgttaca
      121 accacaacga caacggactc aacgactgaa ccggccccgg aaccggatat cgaacaaaaa
      181 aaagcggcct atggcgcact ggcggatgtg ctggataacg acacctcgcg taaagaattg
      241 atcgaccagt tgcgtactgt cgccgcgacg ccccctgcgg aaccggtccc gaagatcgtg
      301 ccgccgacgc tggttgaaga gcaaaccgtg ctgcaaaagg tcaccgaagt cagccgccat
      361 tatggtgaag ccctttccgc ccgcttcggg caactttatc gcaatattac cggctccccg
      421 cataagccgt ttaatccaca aacattcagc aatgcgctga cccatttttc aatgttagcg
      481 gtattagtgt ttggttttta ctggctgatt cacctgtgcg cactgccgct gtatcgcaaa
      541 atgggcctgt gggcgcggca aaaaaatcgt gagcgcagta actggttgca gcttccggcg
      601 atgattatcg gggcgtttat tatcgaccta ctgttactgg cactgacatt atttgtcggc
      661 caggtattaa gcgacaacct gaatgcaggc agtcgcacca tcgctttcca acaaagtttg
      721 tttctcaacg cctttgccct gattgaattt ttcaaagctg tactacgcct gattttttgc
      781 ccaaacgtgg ctgaactgcg cccgttcacg attcatgacg agaccgcccg ttactggagt
      841 cgtcgcctga gctggttaag cagtttgata ggttatggcc tgattgtggc cgtgccgatt
      901 atctctaatc aggtgaatgt acagataggt gcgctggcga acgtcatcat tatgctgtgc
      961 atgaccgtct gggcgttgta cctgatcttt cgcaataaaa aagagattac ccaacatttg
     1021 ctcaactttg cggagcattc gctggccttt ttcagcctgt ttatccgcgc ctttgcgctg
     1081 gtgtggcact ggctggcaag cgcctatttt atcgtgctgt ttttcttttc gttgttcgat
     1141 ccgggcaaca gcctgaaatt tatgatgggt gcaacggtgc gcagcctggc gattattggt
     1201 atcgcagcgt ttgtttccgg tatgttttcc cgctggctgg cgaaaaccat caccctctcg
     1261 ccacatactc agcgtaacta tccggagttg caaaaacggc tgaatggctg gctttcggcg
     1321 gcgctgaaaa cggcgcgtat tctgaccgtc tgcgtggcgg taatgttgct gttgagcgca
     1381 tggggattgt tcgatttctg gaactggctg caaaacggcg cggggcagaa aaccgtagat
     1441 atcctgatcc gtatcgcact tattcttttt ttctcggcgg ttggctggac ggtgctcgcc
     1501 agtttgatcg aaaaccggct ggcttcggat attcatggcc gcccgctacc cagcgcccgt
     1561 acgcgtaccc tgctgacgct gtttcgtaac gcgctggcgg tgattatcag taccatcacc
     1621 atcatgattg tgttgtcgga aatcggcgtc aatatcgcgc cattgctggc aggtgccggg
     1681 gcattaggtc tggctatctc gtttggttcg caaacgctgg tgaaagatat cattaccggg
     1741 gtgtttattc agtttgaaaa cggcatgaac actggagatt tggtgactat cgggccgttg
     1801 accggcacag tggaacggat gtcgattcgc tccgtgggcg tgcgccagga taccggggcg
     1861 tatcacatca ttccgtggtc ttcgataacc acctttgcta acttcgtccg cggtattggt
     1921 tcggtagtgg caaactacga tgttgatcgc catgaagatg ctgataaagc caatcaggca
     1981 ctgaaagatg cggtagcgga attgatggaa aacgaagaaa ttcgcgggct gattattggt
     2041 gaaccgaatt ttgccgggat tgtcggctta agcaataccg cgtttacact gcgtgtttcg
     2101 ttcaccacgc tgccactcaa acagtggacg gtacgctttg ccctcgacag ccaggtgaaa
     2161 aaacatttcg acctggtggg cgttcgcgcg ccagtgcaga cttattaggt gctgcctact
     2221 ccgggcgcga ccccggctga accgttaccg ccgggggagc caacgcttta acgctggcga
     2281 ttgacaaaac ggcggcgctg ctcgtcgttc ataaaggtcc aggcaataaa gcgactctgc
     2341 ttttgcccct gggccatctc tttttttacc accttcaccg cgcccacgtc ggtcagagca
     2401 cgatacaacg gcggtaagtt ttcaccacga gataccagcg aggtaaacca catcacctgc
     2461 ttcgcgaagc ctttgctctc ttcaatcatc tttttgataa aggcgacttc accaccttca
     2521 caccacaact cctgttgctg accaccaaag ttcagtgcat cgtctttgtt cagccccagg
     2581 ttacggcgtt tacgctcact acctgcccgc gctgcagcgg cggaatcgtg gaatggcggg
     2641 ttacacaagg tcgcgtcgta ttgctcgttt ttatggatga tgccgttaaa aattgcccca
     2701 ctctcttttt gccgacgcag acgaatggcg cggttaagcc ccggattagc actgataatc
     2761 gcctgcgcac tgcttaacgc ctggctgctg gtttcactac cggtaaaacg ccagccatat
     2821 tcatgtacgc caatcagcgg ataaatacag ttcgcaccaa cgccgatatc cagaatgctg
     2881 gcattcgctg gaattgttcc gcttgcttca gccagtaaat cggcaaggtg atgaatataa
     2941 tccgcccggc cgggtactgg tgggcagaga aaaccgtcgg ggatatccca gttcgctacg
     3001 gcgtaaaaat gggccagcaa cgccttattg agcgccttca ccgccagcgg attggcaaag
     3061 tctacgcttt gctccccggc gggtgtaagc gtgaggaatt gcctgagttc aggattgacc
     3121 tgacaaagcg tggcgagatc gtagcggctg tgatgacggt tgcgcggatg caaccccggt
     3181 ttctgggcgg acatagcatt ctcctttaag cagcggcgta agatacccgc ataacgcgac
     3241 agggtaaata tctggctgcg tttatcattc cccaaaacct taacatggct atggtttatg
     3301 gcaattcatc ataaaaatca gcattatgca tgtcattgat ttgtcttatt ttgtaaaaat
     3361 atatcgttca aaaaataagc acatctctgt caattttttg cgtcatttgc atcgcgcagg
     3421 cgacctacac ttatcaggca ctacccgttg tatttatgag gatgtcatta tgaaaaagtg
     3481 cctcacacta ctgattgcca ccgtcctgag cagaatctct ctcacggctt atgccgcgca
     3541 accgatgagt aacctggaca gcggtcagct acggcccgcg gtaccgtttc ggcaactggc
     3601 gcatcaaacc taagcgatct ggaggataaa ctggcagaaa aagcgcgcga acaaggcgcg
     3661 aaaggttatg tcattaattc cgcaggcgga aatgaccaga tgttcggtac tgcaaccatc
     3721 tacaaataac cccaccaacg taactgcatt cgcctctgga tgcagttttc gcctgttttg
     3781 taatcaaaaa acatcaaata tgaactcaat gtaaataaat gtatttcttt ttcgcgcaat
     3841 gggtgataga aaatcgcgcc aagtgataat gtttatcaaa attattatca cttgcatgaa
     3901 cactatcacg ggattaacag tggcatcgca tccgcagaga tgctttctcg tgacagtgaa
     3961 aatttcaaca tataagaaaa agtcacctgc aaaatggaaa acaatcgcaa tttccctgcc
     4021 agacaatttc attcgctcac gttctttgcc ggtctttgta ttggcatcac gcctgtggct
     4081 caggcactcg ccgccgaagg gcaaactaac gcggatgaca cgctggttgt cgaagcatca
     4141 acgccttcgc tttatgcgcc acaacaatct gccgatccga aattctcgcg tccggtagcg
     4201 gatactaccc gcacgatgac ggtgatttct gaacaagtga ttaaagatca gggcgcaacc
     4261 aaccttaccg acgcgctcaa aaacgtcccc ggcg
//
LOCUS       DADLFA010000241         4267 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000241 DADLFA010000000
VERSION     DADLFA010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4267
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.243"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..212)
                     /locus_tag="JQH65_003804"
     CDS             complement(<1..212)
                     /locus_tag="JQH65_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HAY6141062.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPV"
     gene            522..2294
                     /gene="aspS"
                     /locus_tag="JQH65_003805"
     CDS             522..2294
                     /gene="aspS"
                     /locus_tag="JQH65_003805"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAY6141063.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2412..2864
                     /gene="nudB"
                     /locus_tag="JQH65_003806"
     CDS             2412..2864
                     /gene="nudB"
                     /locus_tag="JQH65_003806"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HAY6141064.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDSQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2893..3633
                     /locus_tag="JQH65_003807"
     CDS             2893..3633
                     /locus_tag="JQH65_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY6141065.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVITFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEIAATL"
     gene            3668..4189
                     /gene="ruvC"
                     /locus_tag="JQH65_003808"
     CDS             3668..4189
                     /gene="ruvC"
                     /locus_tag="JQH65_003808"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HAY6141066.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
BASE COUNT         1053 a         1061 c         1199 g          954 t
ORIGIN      
        1 tcaaccggct gttttaatgc ttcggcgtaa tcggcagacc agccaactcg caccagaaac
       61 acgggctgac cgctggcgcg aaatttcgcc gccagcttcc cggcgcgatt aactacctca
      121 tcggcagtat gtggaccacc ggcaaaaggt aagatgcctt cttgtaaatc gatcaccacc
      181 aacgcagtgg ttttagcatt aagttcaagc atgctgactc ctgaaaaaac aaaatcgtgc
      241 ctcacacctt aatgcgatta gccgatggcg gaacgacaaa ttttgttaat ttttatgagt
      301 tcgggcaaca cctttatagc aaactggcgt agagtctgat ttcaggcccg atataaagtg
      361 gtaacgaggc gttccagcgc gggctaggct cttacggtgc ggcagaattt ccagtataat
      421 agccgccttt tttcattcag ttgtgacata cagctaacgc tgcgacttgg tcacctgcgc
      481 agcagggggc atccgccggc agctaagtta agggatatct catgcgtaca gaatattgtg
      541 gacagctccg tttgtcccac gtggggcagc aggtgactct gtgtggttgg gtcaaccgtc
      601 gtcgtgatct tggtagcctg atcttcatcg atatgcgcga ccgcgaaggt atcgtgcagg
      661 tatttttcga tccggatcgt gcggacgcgt taaagctggc ctctgaactg cgtaatgagt
      721 tctgcattca ggtcacgggc accgtacgtg cgcgtgacga aaaaaatatt aaccgcgata
      781 tggcgaccgg cgaaatcgaa gtgctggcgt cctcgctgac tatcatcaac cgcgcagatg
      841 ttctgccgct tgactctaac cacgtcaaca ccgaagaagc gcgtctgaaa taccgctacc
      901 tcgacctgcg tcgtccggaa atggctcagc gcctgaaaac ccgcgctaaa atcaccagcc
      961 tggtgcgccg ttttatggat gaccacggct tcctcgacat cgaaactccg atgctgacca
     1021 aagccacgcc ggaaggcgcg cgtgactacc tggtgccttc tcgtgtgcac aaaggtaaat
     1081 tctacgcact gccgcaatcc ccacagttgt tcaaacagct gctgatgatg tccggttttg
     1141 accgttacta tcagatcgtt aaatgcttcc gtgacgaaga cctgcgcgct gatcgtcagc
     1201 ctgagtttac tcagatcgat gtggaaactt ctttcatgac cgcgccgcaa gtgcgtgaag
     1261 tgatggaagc gctggtgcgt catttgtggc tggaagtgaa gggcgtggat ctgggcgatt
     1321 tcccggtaat gacctttgcg gaagcagaac gccgttatgg ttctgataaa ccggatctgc
     1381 gtaacccgat ggaactgacc gacgttgccg atctgctgaa atctgttgag tttgcagtat
     1441 ttgcggggcc ggcgaacgat ccgaaaggcc gtgttgccgc tctacgtgtt ccgggtggcg
     1501 catcgctgac ccgtaagcag atcgacgaat acggtaactt cgttaaaatc tacggcgcga
     1561 aaggtctggc ttacatcaaa gttaacgaac gcgcgaaagg tctggaaggt atcaacagcc
     1621 cggtagcgaa gttccttaat gcagaaatca tcgaagccat cctggatcgt actgccgcgc
     1681 aagatggcga tatgattttc ttcggtgccg acaacaagaa aattgttgcc gacgcgatgg
     1741 gcgcactgcg cctgaaagtg ggtaaagacc ttggtctgac cgacgaaagc aaatgggcac
     1801 cgctgtgggt tatcgacttc ccgatgtttg aagacgacgg tgaaggcggc ctgacggcaa
     1861 tgcaccatcc gttcacctca ccgaaagaca tgactgctgc agaactgaaa gctgcaccgg
     1921 aaaatgcggt ggcgaacgct tacgatatgg tcatcaatgg ttacgaagtg ggcggtggtt
     1981 cagtacgtat ccataatggt gatatgcagc agacggtgtt tggtattctg ggtatcaacg
     2041 aagaggaaca gcgcgagaaa ttcggcttcc tgctcgacgc cctgaaatac ggtactccgc
     2101 cgcacgcagg tctggcattc ggtcttgacc gtctgaccat gctgctgact ggcaccgaca
     2161 atatccgtga cgttatcgcc ttcccgaaaa ccacggcggc agcgtgtctg atgactgaag
     2221 caccgagctt tgctaacccg actgcactgg ctgagctgag cattcaggtt gtgaagaagg
     2281 cagagaataa ctgatatgac tcaaatacac gaaatcattc gcgttgcatc aaggcggcaa
     2341 ttgagtgaac tcccatgagc atagataact atgtgaatgg gatgagcgaa ggcagtcaac
     2401 gaagaggcag cgtgaaggat aaagtgtata agcgtcccgt ttcgatctta gtggtcatct
     2461 atgcacaaga tacgaaacgg gtgctgatgt tgcagcggcg tgacgatccc gatttctggc
     2521 agtcggtaac cggcagcgtg gaagagggtg aaaccgcgcc gcaagctgcc atgcgcgaag
     2581 taaaggaaga ggtcaccatt gatgttgtcg ctgaacaact gaccttaatt gacagtcagc
     2641 gcacggtaga gtttgaaatt ttttcacatt tacgtcatcg ctatgcgccg ggcgtgacgc
     2701 gtaatacgga atcatggttc tgtcttgcgc ttccgcacga gcggcagatc gttttcactg
     2761 aacatctggc ttacaagtgg cttgatgcgc ctgctgcggc ggcgctcact aagtcctgga
     2821 gcaaccggca ggcgattgaa cagtttgtaa ttaacgctgc ctgaacaggc agggctatct
     2881 ggagatattt ttatggcagg tcatagtaaa tgggccaaca ccagacatcg taaagctgcg
     2941 caggatgcta agcgcggtaa aatcttcact aaaatcattc gtgagctggt aaccgcggct
     3001 aagctgggcg gtggcgatcc ggacgctaac ccgcgtctgc gtgcggcggt ggataaagca
     3061 ctgtctaaca acatgacccg tgacacactg aaccgcgcaa ttgcacgtgg tgtgggcggt
     3121 gatgatgatg caaacatgga aaccatcatc tacgaaggtt acggtcctgg cggcacggca
     3181 atcatgattg aatgtctgtc tgacaaccgc aaccgtaccg ttgctgaagt gcgtcatgca
     3241 ttcagcaaat gtggcggtaa cctcggtaca gatggttccg tagcctatct gttcagcaaa
     3301 aaaggcgtga tcaccttcga gaaaggcgat gaagacacca tcatggaagc agctctggaa
     3361 gcaggtgctg aagacgttgt gacctatgat gacggcgcga ttgatgtcta cactgcatgg
     3421 gaagaaatgg gtaaagtgcg cgacgctctg gaagcggcag gtctgaaagc agacagcgcg
     3481 gaagtttcca tgatcccgtc caccaaagct gatatggatg cagaaaccgc accgaaactg
     3541 atgcgtctga tcgatatgct ggaagattgc gacgacgtgc aggaagttta ccataacggt
     3601 gaaatctctg atgagattgc agcgactctc tgatgaggcc tgctaaacag caaaacggag
     3661 acgcgtgatg gctattattc tcggcattga cccgggttcg cgcgtgaccg gctatggcgt
     3721 catccgccag gtaggcaggc aactgtccta cctgggtagc ggatgcatac gcaccaaggt
     3781 ggatgattta ccgtctcgtc tgaagctcat ctatgcgggc gtgacggaaa tcatcaccca
     3841 gttccagcca gattatttcg ccattgaaca agtctttatg gccaaaaatg ccgactcggc
     3901 tctgaaactg gggcaggcgc gcggcgtggc gattgtggcg gcggtgaatc aggaattgcc
     3961 ggtatttgaa tatgcggcac gtcaggtaaa gcaaacggtg gtaggtattg gtagtgccga
     4021 aaaaagtcag gtgcagcata tggtccgcac cttgctgaaa ctgcccgcta atccacaggc
     4081 ggatgccgcc gatgcgttag ctatcgcgat tacccattgt cacgtcagcc agaatgcaat
     4141 gcagatgagc gaatcgcggc tgaacctggc gagagggcga ctgcgttaag ttataccgcc
     4201 tcggtcagtt caggctgagg cgtttcaact ccctccgcgt gaatcaacac caaacccgga
     4261 tgatgcc
//
LOCUS       DADLFA010000242         4203 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000242 DADLFA010000000
VERSION     DADLFA010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.244"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            112..615
                     /gene="yeaK"
                     /locus_tag="JQH65_003809"
     CDS             112..615
                     /gene="yeaK"
                     /locus_tag="JQH65_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HAY6141067.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     VNFRRTA"
     gene            complement(616..720)
                     /locus_tag="JQH65_003810"
     CDS             complement(616..720)
                     /locus_tag="JQH65_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141068.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            889..1335
                     /locus_tag="JQH65_003811"
     CDS             889..1335
                     /locus_tag="JQH65_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAY6141069.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(1292..2113)
                     /locus_tag="JQH65_003812"
     CDS             complement(1292..2113)
                     /locus_tag="JQH65_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY6141070.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            2210..3406
                     /gene="nimT"
                     /locus_tag="JQH65_003813"
     CDS             2210..3406
                     /gene="nimT"
                     /locus_tag="JQH65_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005117270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HAY6141071.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGSNSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGNWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     RSYPVL"
     gene            3446..3793
                     /locus_tag="JQH65_003814"
     CDS             3446..3793
                     /locus_tag="JQH65_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HAY6141072.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFTTFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(3815..4069)
                     /gene="yoaF"
                     /locus_tag="JQH65_003815"
     CDS             complement(3815..4069)
                     /gene="yoaF"
                     /locus_tag="JQH65_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HAY6141073.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVSTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
BASE COUNT          967 a         1065 c         1043 g         1128 t
ORIGIN      
        1 catgatgcgt ataaagcttc cgatgagcgt ttatatgtca ataagcagaa caaaaacagc
       61 cgttcatgat aaccttctgt ggttgtttgc ttgtaatctc aggagcgtga aatgactgaa
      121 atggctaaag gaagcgtgac gcatcagcgg ttaatcgctt tattatcaca agaaggtgct
      181 aacttccgcg tcgttaccca tgaagcggta ggtaaatgtg aagcggtgtc agaaattcgt
      241 ggcaccgctt taggccaggg agctaaggca ctggtatgta aagtcaaagg caatggcgta
      301 aatcaacatg ttctggcgat cctcgctgcc gatcaacagg ccgatctgag ccaacttgcc
      361 agccatatag gcggattacg cgcttcgtta gccagccctg ctgaagtcga tgaactcaca
      421 ggctgtgtct tcggcgcgat cccccctttc agcttccatc caaaactcaa actggttgcc
      481 gaccctctac tcttcgaacg atttgatgaa atcgccttca atgcagggat gctggataaa
      541 tccgttattt tgaaaaccac ggattatttg cgcattgcgc aaccagaact ggtcaatttc
      601 cgccgcactg cgtaactagc ctgtccgttc aataagcaga acggacaaaa ccagcacaac
      661 agcaatggca aaaaacgatg acgtgataat cagtgtctcg acgaacattt gatcgttcat
      721 agcatgcccc cgggagatag ctgttttcac gttattgtta gcgtgcacaa atggcagttt
      781 gatgacagtt cgcctgattt ttatgcaaaa aacgtaaaga ttttctactt ccttcctgca
      841 gcaagcgtaa agtaagcagg cttattattt tttggcaagg aaaccacgat gtttgatgtc
      901 actttgctga tcctgctcgg gttagctgcg ctgggcttta tcagtcataa caccactgtc
      961 gccgtttcaa ttctggtgtt gatcattgtc cgcgtcacac cgttaagcac ctttttcccg
     1021 tggattgaaa aacaggggct aagtatcggg ataatcattc tgacgattgg cgttatggcc
     1081 cccatcgcca gcgggacgct accgccctca accttgattc actcgtttct gaactggaaa
     1141 tcgctggtgg caattgcagt aggcgtgatt gtctcctggc tgggtgggcg cggcgtgacc
     1201 ttaatgggca gccagccgca actggtcgcc gggctgctgg taggcactgt tttaggcgtg
     1261 gcgctgtttc gcggcgtacc ggtcggcccg cttattgccg cgggtctggt ttcgctgatt
     1321 gtggggaaac agtagttaat cccgcaatat atcggcctgg cgtttgcccc aggcctttct
     1381 taaacatagt aataaaggcc gttgttgaat cataccccag cgtatgagca actttctgta
     1441 cagtatcgcc tttcactaat ccctgaagtg ccataatcag ctgtaactgc tgacgccact
     1501 ggcgaaaact caaccccgtc tcttttacga tcagtcgcgc aagattgcgt tcactcatcg
     1561 caaaaaagcc agcccattgc cctaatgacc cccactcgac gggccctttc gccatcatct
     1621 ccaccatcgt gcggatttta ggatgagaag aaacgggcag gtgcagctgt tgttgtggct
     1681 gctgagggag ttcatcaaac aggacctgta ttaatctgcg ggtcatcggc tcagcacgct
     1741 gagtcgtcgt cctgtttgcc agggttaaaa tcaattcccg gcataagggc gatattttca
     1801 atgtgcaaca tgttgtcggc attgtcactg cgctgggttc gataaacaga aaacagagtt
     1861 cagcgttagc tgtcacttga ttgctatgtt ccaccccgcc cggtatccaa accgcatatt
     1921 gcggcggcac catccacaaa gcattttcca ccgtacaggt aattgcacca tgtaatgcta
     1981 gtatcagctg tcctttgcgg tgctgatgta caggactggt taactcatcc gttccggcgt
     2041 gaatacaaaa tgcgaccgcg gcttcatggt gtcggtcggg ttcatagcca ttgagattca
     2101 acctgtgcat cattttgtcc gaatttagcg ataatttgtc attttagctt gattcaacat
     2161 aacaataaaa acggtaaggt acagcctcgt ttgtaacaat gagaagcata tgacctgttc
     2221 aacttcatta agcggcaaaa acaggattgt ccttatcgct ggcattctga tgattgccac
     2281 aacattacgc gtcaccttta ccggcgcagc accgttactg gatacgattc gttccgctta
     2341 ctcgctgacg acagcgcaaa ccggcttatt gaccaccctg ccattattgg cctttgcgct
     2401 aatctcacct ttggccgccc cggtagcgcg acgttttggt atggaacgta gcctgtttgc
     2461 cgcgttactt ttgatctgtg ctggtatcgc aattcgctct ctcccttcgc cttacttatt
     2521 atttggcggt acagcggtca ttggcggtgg gattgcatta ggcaatgtct tactgccagg
     2581 attaattaaa cgcgatttcc ctcattccgt cgccagactt accggcgcat attccctgac
     2641 aatgggagct gcggcggcac ttggatcggc tatggtagtg ccgctggctt tgaacggttt
     2701 tggctggcaa ggcgcgttgc tcatgctgat gtgttttcct ctgctggctc tttttttatg
     2761 gctgccacag tggcgaagcc aacaacatgc aaatttgagt acctcgcgcg ccttacatac
     2821 tcggggtatc tggcgttcgc cgcttgcctg gcaggtcaca ttgtttcttg ggagcaactc
     2881 actggtctat tacgtgatta ttggctggct tccggcgatc ctcatcagtc acggctatag
     2941 cgaagcacag gcgggttcac tgcatggttt gctgcaacta gccacagcag ctcccggttt
     3001 gctgatccct cttttcttac atcatgtgaa agaccagcgt ggtattgcag cgttcgttgc
     3061 cttgatgtgc gcagtgggcg ctgttgggct ctgctttatg ccagcgcacg cgatcacctg
     3121 gactctgctt ttcggttttg gttccggcgc aacaatgata ctggggttga cgttcattgg
     3181 tcttcgggca agttctgcgc atcaggcggc ggcactctcg gggatggcac aatccgtcgg
     3241 gtatttgctg gcagcctgtg ggccgccgct aatgggtaaa atacacgatg ctaacggtaa
     3301 ctggtccgta ccactgatgg gtgttgcaat actttcacta ctgatggcga ttttcggact
     3361 ttgcgccggg agagacaaag aaattcgctc atatccggtg ctatagtgac gtgacaaatc
     3421 atgcgtgaaa gggagaacaa acacgatgaa tattcagtgc aaacgcgttt atgatccggc
     3481 tgaacagagc gatggttatc gcgtactggt cgaccgcctc tggccgcgcg gtatcaaaaa
     3541 aaccgattta gcccttgatg agtgggataa agaaatcacg ccgtcaacgg aactgcgcaa
     3601 agcctttcac ggcgaagtcg tcgattttac aacctttcgc gagcaatatc ttgcagaact
     3661 ggcgcaacac gagcaagaag gaaagcggct ggcggacatc gccaaaaaac agccgctgac
     3721 cctgctctac gcagcaaaaa acaccacgca gaaccatgcg ctggtgctgg ccgactggct
     3781 acgtagcttg tgattttagt acagcatccg gcggttattt ttcaccagcc ggatggtccc
     3841 gtcgccacaa tgcccattca tcaatcgttt caccgcccgg taatttgcaa ttgttgctga
     3901 ccccttgcgc tgtcgacact ggaatgagcg tcccgccctt ctgctggcaa tagaccgacg
     3961 ccggatttgc cataccaatc tgcggcggtt taggtgcttc tggctgagaa ggggttgaac
     4021 aaccagccag gaccagcaag caaggcagaa caaaactgat aattttcatt tattgatctc
     4081 acatatttat ccagacttag agtatcgcgg tatcgttttg ttttgcagca ctatttttat
     4141 tacattcact caaaacatat tacgtcttgt ttcatctttg ttgatgatgt tttatcatgc
     4201 ctg
//
LOCUS       DADLFA010000243         4183 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000243 DADLFA010000000
VERSION     DADLFA010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.245"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..209)
                     /locus_tag="JQH65_003816"
     CDS             complement(<1..209)
                     /locus_tag="JQH65_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAY6141074.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="JQH65_003817"
     tRNA            complement(324..399)
                     /locus_tag="JQH65_003817"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="JQH65_003818"
     tRNA            complement(406..480)
                     /locus_tag="JQH65_003818"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="JQH65_003819"
     tRNA            complement(597..681)
                     /locus_tag="JQH65_003819"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="JQH65_003820"
     tRNA            complement(690..765)
                     /locus_tag="JQH65_003820"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="JQH65_003821"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="JQH65_003821"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAY6141075.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAAQRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="JQH65_003822"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="JQH65_003822"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HAY6141076.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="JQH65_003823"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="JQH65_003823"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAY6141077.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQYATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLLKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1078 a         1041 c          933 g         1131 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctggaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagcgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc gacacgatga agaaacagcc gaatggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttcttcttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctaggagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctcagcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctctg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg tagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaagtc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcggaatg gattatccac acgatccaca tcatgtattt gtttctgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgtttcct
     1861 gaaattccgc gaaggggctt ttaccgaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgcg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcgagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agcttgcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggtactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttagcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catactgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagtt atgacgacag ggagagtttg tag
//
LOCUS       DADLFA010000244         4111 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000244 DADLFA010000000
VERSION     DADLFA010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4111
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.246"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(953..1144)
                     /locus_tag="JQH65_003824"
     CDS             complement(953..1144)
                     /locus_tag="JQH65_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HAY6141078.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
     gene            1209..1532
                     /locus_tag="JQH65_003825"
     CDS             1209..1532
                     /locus_tag="JQH65_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAY6141079.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPT
                     LAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1474..2232
                     /locus_tag="JQH65_003826"
                     /pseudo
     CDS             1474..2232
                     /locus_tag="JQH65_003826"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2210..2740
                     /locus_tag="JQH65_003827"
     CDS             2210..2740
                     /locus_tag="JQH65_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HAY6141080.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNESLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            2747..2980
                     /gene="mbeD"
                     /locus_tag="JQH65_003828"
     CDS             2747..2980
                     /gene="mbeD"
                     /locus_tag="JQH65_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HAY6141081.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNEQVMQLSQQVQHLSERTERLSQLYSENRR"
     gene            complement(3528..3995)
                     /locus_tag="JQH65_003829"
     CDS             complement(3528..3995)
                     /locus_tag="JQH65_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141082.1"
                     /translation="MRIDISHQTRHTPPNMLPREQNCVAMALSACFRQQLNPVVNSLL
                     KERIIHSPKELEHDNAVIRALQKLQIQEVCNSTLWETAKQQLLQKSDGRYFAINSKHL
                     AFPGPGESHAFCCIKYKNAIGINGNNAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT          984 a          933 c         1226 g          968 t
ORIGIN      
        1 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt acagttacca
      181 atggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggatgagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgactag agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccatcggcg cggccttctt cctgttcctg gtcttttgct cacatgttct ttccggcgtt
      601 atcccctgat tctgtggata acagcatttc gtttttgagt gagtagatac cgctcgccgc
      661 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg cctggacgtg
      721 cattttctcc ttacgcatct gtgcggcatt tcacacccgg tatggcgcac ttttcataca
      781 atccgcactg atgccgcatg gtgaagccag tatacactcc gctatcgcta cgtgactggg
      841 tcatggctgc gccccgacac ccgctaaaac ccgctgacgc gccctgacgg gcttgtctgc
      901 tcccggcatc cgctcacaga taagctgtga ccgtctccgg gagctgcatg cgtcagaggt
      961 tttcaccgtc atccccgaag cgagcgaggc aactgcggta aagctcatcg gcgtggtcgt
     1021 gaagcgattc acagatgtct gcctgttcat ccgcgtccag ttcgttgagc ttctccagca
     1081 gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttag
     1141 tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac
     1201 gcgagaggat gctcacgata cgggttactg atgatgaaca tgctcgtttg ctggaacgtt
     1261 gtgagggtaa acaactggcg gtatggatgc ggcgggtctg cctgggggag ccggttgccc
     1321 gttccgggag gctgccgaca ctggcaccgc cgttactgcg tcagctggcc gccatcggga
     1381 ataacctgaa tcagacagcg aggaaggtga acagcgggca gtggtcttcc ggtgaccggg
     1441 ttcaggtggt ggccgcactg atggccatcg gggatgagct gcgccggctg cgtctggccg
     1501 tcagggaaca ggggacgcgg gatgatagtt aagtttcatg ccaggggaaa aggtggtggc
     1561 agtggtccgg ttgattacct gctgggaagg gagcgtaacc gggaaggggc aacggtgctt
     1621 cagggtaatc cggaagaagt ccgggaactc atcgatgcca cgccatttgc gaagaaatac
     1681 acgtccggtg ttctctcgtt cgcggagaag gcgctgccag cgggaggacg tgaaaaagtg
     1741 atggcgagtt ttgagcgtgt actgatgccc ggtctcgaaa aaaatcagta cagcatcctg
     1801 tgggtggagc accaggacaa gggacggctt gagctgaatt ttgtcattcc gaacatggag
     1861 ctacagaccg gaaaacgcct ccagccgtac tacgaccggg cagacaggtc gagaattgat
     1921 gcctggcaga cgctggtgaa tcaccattac gggctgcatg acccgaacgc cccggagaac
     1981 cgccggacgc tgactctccc tgataacctg ccggaaacga aacaggcgct tgctgagagc
     2041 attacacggg ggatagacgc tctttatcat gtcggggaga taaaagaccg tcaggatgtg
     2101 attcaggcgc tcacagaggc ggggcttgaa gtggtcaggg tgacgcgaag cagtatcagc
     2161 attgcagacc cgaacggcgg gaagaatatc agactgaaag gagcattcta tgagcaatct
     2221 tttacagacg ggcgcggagt tcgagaaaaa gctgaaagag agagccgaat ctacagagaa
     2281 aatgctgaac gacgagttca ggaagctcga agaatctgta agcaaggctg tgacatcaaa
     2341 cgagacgaaa atcaaagacg ctatagccct gttcacagct tcgacagagg aatcactgaa
     2401 aaaacaccgg gaaggggtga aagaggcgat gatgcagcac aggaaggacg tgttaaagct
     2461 ggcagggaat acgggcatga tgttgctggg gatggttatt ttcctgttta ccgtgagtgg
     2521 cgggacgctc tggtatcttg gcgggatgat acaggcgaac ctggaagaaa tcaggaagca
     2581 gaacgagagt ctggagaagc tgaacgcgaa gacatggggc gtgcgttatc acgaggggag
     2641 caacggacgt tttctggtgt tgcccgaggg gatgaaagcc gaaacgaact ggacgatgga
     2701 aaacgggaaa cagaatgccg taaggctggt acaggagtaa cagagcatga cggagctgga
     2761 aagacatttg ctgaacgcat tagagcaact gcaacaggac tatatgcagc ggctgaacga
     2821 atgggagagc gccttcgtgg aattgcagaa aatgttttcg cttacgcaac gggacaacgc
     2881 gatgctgaac gagcaggtca tgcagttgag tcagcaggtg cagcacttga gcgagcggac
     2941 agaacgcttg agccagttat acagcgagaa caggagataa gggacgaacg gctgatacag
     3001 gaatgcgaac atgagttatc cctggagcgt gagcgccagc cggaaataca ggaacgcaca
     3061 ctggatggtc cttcactggg atggtaatca gagacaggct aaaatgttgt cagcgcgttt
     3121 ccggtgcatt ttttgggata atggttggtt cgggggggca ccgggaacgc gcttcagtca
     3181 gaccttgctg aaaacacagg aacgcgttaa aacgcatacg ggcgcgattc tggtgtgtct
     3241 tttgttgctg tgttttgcct ctattgccag atcgcgctca ttcccctgaa cttctgtttt
     3301 cccgctcagg tccccgtctt cttcgcttcc gaaagcatga aaaaaatccg tttcagctgt
     3361 ctctggctgg caattcctgc gtgattcaca tggctgcata gctatgcatg tggttaaaat
     3421 ttaccgggtg cgatcgcggc agtttttcgg gtggtttgtt gccattttta cctgtctgct
     3481 gccgtgatcg cgccgaacgc gtttcagcgg tgcgtacaat taagatatta cgttaaattg
     3541 tgagggaatg gtccccatat actgacttta tcatagggat agggttggta gtgggtcgat
     3601 tgggtttcgg cattgtttcc gttaatgcca attgcatttt tgtatttgat gcaacagaag
     3661 gcgtgagatt ctcctgggcc tggaaaggct aagtgtttag aattgatggc aaaatatctt
     3721 ccgtccgatt tttgaagtag ttgttgtttg gctgtctccc aaagggtgct gttacaaact
     3781 tcttgaattt gtagtttttg cagggctctt attactgcgt tgtcatgttc caattcttta
     3841 ggggagtgta ttattctctc ttttaataag ctgtttacca cagggtttag ttgttgtcta
     3901 aagcacgcag ataatgccat tgctacacaa ttttgttccc gaggaagcat gtttggcggg
     3961 gtatgccgtg tttgatgtga tatgtctatt ctcatagaag gtctctggct tatagcatca
     4021 acaaatacgg ctataaaaca aaattttcat agagtgattc aagatcttct tgagatcctt
     4081 ttttgcggcg cgtaatctgc tgcctgcaaa c
//
LOCUS       DADLFA010000245         4081 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000245 DADLFA010000000
VERSION     DADLFA010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.247"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            33..932
                     /locus_tag="JQH65_003830"
     CDS             33..932
                     /locus_tag="JQH65_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY6141083.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            1269..2882
                     /locus_tag="JQH65_003831"
     CDS             1269..2882
                     /locus_tag="JQH65_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY6141084.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRIMYIVKH"
     gene            complement(2933..3964)
                     /gene="ynaI"
                     /locus_tag="JQH65_003832"
     CDS             complement(2933..3964)
                     /gene="ynaI"
                     /locus_tag="JQH65_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HAY6141085.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
BASE COUNT         1145 a          896 c          961 g         1079 t
ORIGIN      
        1 taaatgatta atagattgca tggagatact gaatgaagcg tgaagaaatc gctgatctga
       61 tggcgtttgt cgtcgttgca gaggagcgta gcttcactcg tgcagcagcc cgcctgagca
      121 tggcgcagtc agctttaagc cagatagtgc gtcgtataga agaacgattg ggattgcggc
      181 ttctgacgcg aaccacgcgc agcgttgttc caactgaagc gggcgagcat cttttgtctg
      241 ttcttggccc tatgttgcat gacatagatt cagccctggc atccctgagc gagctgcaga
      301 accgcccatc cgggacaata cgtattacta ctgtagaaca tgcagcaaaa acgatattgt
      361 taccagcaat gcgcacattc ctgaaatcgc atcctgaaat tgatattcag ctcaccattg
      421 attatggttt gaccgatgtc gtttctgaac gttttgatgc aggcgtccgt ctgggtgggg
      481 agatggataa agatatgatc gccattcgaa tcgggccaga tataccaatg gctattgttg
      541 gctcaccgga ttatttttct cgccgaagtg ttccaacgtc agtgtcacaa ttaatagatc
      601 atcaggcaat taatttgtat cttcccacat cgggtacagc aaatcgctgg agattaatac
      661 gcggtggacg tgaagttcgt gttcgcatgg aaggtcagct tttactgaat acgatagacc
      721 tgatcattga tgctgcaatt gatgggcatg gattggcgta tctaccttat gatcaggttg
      781 agcgggctat taaagaaaaa aaactgatac gtgttttgga taaattcaca ccagatttac
      841 ccggttatca cctgtactat ccacaccgtc gacatgctgg ctcggcattc tcattattta
      901 tagataggct gaagtataaa ggtgctgttt agcactactt gctgatacat taatttaatt
      961 tctctcttaa cgtattctca gttcctttca acgttttggt tattttttat tcttcgtaca
     1021 atggcgacag atgctgattt tgataccgaa aactggtttg aacgtgcgaa gcctgaacgt
     1081 agtattcggt gttctatgtg ctttgccgca ttttggcgtc gattatttac actaaatctg
     1141 atttaatata ttgatacata aaacatttta tgcttcctgt gtcacttttt tgatggaagt
     1201 tgtttgcatt tctttaaggc gaaacaaata attacgcatc aattttaatg tcggttagag
     1261 ggaaacttat gaagcactct gtttcagtca cgtgttgtgc gctgttggtc agcagcattt
     1321 ctctttcgta tgctgcagaa gttccgagcg gcacagtact ggcagagaag caggagctgg
     1381 tgcgccacat taaagatgag cctgcgtcgc tggatcccgc taaagccgtg ggcctgccag
     1441 agattcaggt cattcgcgat ctgtttgaag gactggtgaa tcagaacgag aaaggggaga
     1501 ttgtccccgg cgttgcgact cagtggaaaa gtaatgacaa ccgtatctgg acttttaccc
     1561 tgcgcgataa cgcaaaatgg gcggatggca caccggtaac ggcgcaagat tttgtctaca
     1621 gctggcagcg tctggtggac ccgaaaacgc tatcgccatt tgcatggttt gccgcgctgg
     1681 cgggaatcaa caacgcacag gcgattattg atggtaaagc tacgcctgac cagcttggcg
     1741 tcaccgcagt tgatgcccat accttgaaaa ttcagcttga taaaccgttg ccgtggtttg
     1801 tgaatttaac cgctaacttt gccttcttcc cggtgcaaaa agccaacgta gaaagcggta
     1861 aagagtggac gaaacccgga aatctgatcg gcaatggcgc ttatgttctt aaagaacgcg
     1921 tagtcaatga aaaactggtc gtggtaccga atacccatta ttgggataac gccaaaacgg
     1981 tactgcaaaa agtgaccttc ctgccaatta atcaggaatc cgcagccact aagcgttacc
     2041 tcgcggggga tattgatatc accgaatcct tcccgaaaaa tatgtatcag aagctgttga
     2101 aggatattcc ggggcaggtt tatacgccgc cgcagctcgg gacctattat tatgcgttta
     2161 acacgcaaaa agggccgacg gcagatcagc gcgttcgcct ggcattaagt atgacgatag
     2221 atcgccgcct gatgaccgaa aaagtgttgg gaacgggcga aaagccagcg tggcatttta
     2281 caccagatgt taccgccgga tttacgccgg aaccttcgcc gtttgaacaa atgagtcagg
     2341 aagaactgaa tgcgcaggca aaaactttgt tgagcgcagc tggttatggt ccgcaaaaac
     2401 cgctgaagct gacgcttttg tataacactt cagaaaacca tcaaaaaatt gcgattgctg
     2461 tagcatcgat gtggaaaaag aaccttggcg tagatgttaa attgcaaaat caggaatgga
     2521 aaacctatat cgatagccgt aacaccggca attttgatgt tatccgcgcc tcatgggtgg
     2581 gggattataa tgaaccctcc actttcctga cattattaac gtcaacgcat tcaggaaata
     2641 tttcacgctt taacaatccg gcatacgaca aagttctggc ccaggcatcg acggaaaata
     2701 ccgttaaagc gcgtaatgcc gattacaacg cggcagaaaa aatcctcatg gagcaagcac
     2761 cgattgcacc aatttatcaa tataccaatg gacgattaat caagccgtgg ctgaaaggtt
     2821 atcccattaa taacccggaa gatgtggcgt acagccggat tatgtatatt gtgaagcatt
     2881 gatgtgatgg gaactggcgt tacccttgtg gataacgcca gtgatgtctg atttagcgcc
     2941 cctgttccgg cggtgtaatg ttatccatat acagcgtctg gctggggaag gcaaagtccg
     3001 cgccgtgtga ctgcacaata tcgataatct tcaaataaac gtcttgttgt gcagcaagcc
     3061 attcagccca taccgtggtt ttggtaaagc aataaaccat aatattcaat gacgagtcgg
     3121 caaactggtt gaaataaacc agtaaggttt gtcgctggtc gatggccggg tgatttttca
     3181 gcatctcacg tacggcttcg acaataacgc ccacttttgc cgcatcctca taacgtaaac
     3241 caatggtcgt ggtaatgcgg cggttggtca ttcgtcctgg gttttctacg ctgatcgacg
     3301 aaaacagcga gttcggtacg tacaatggac gattatcaaa ggtcgtaatt ttggtaattc
     3361 gccagccaat ttccgctact gtaccttcga tatttctgtc cggtgaacgg atccagtcgc
     3421 caatactgaa aggacggtcg aaatagagca tgatcccgga aaagaaatta ctcagaatat
     3481 ctttaccggc cataccgaca gccagaccac caataccacc aaaggtcagc aagccagaaa
     3541 ggctcatgcc gaaatgttcg ccataaagca gaacaagcac cacaataatg gtgattttga
     3601 taatgcgcga cataatccgc gcactggtga tatcgcgacc ttttttaatc tgctgttttt
     3661 caaactgatt aatcagcaaa aatagcttaa tcgtcagaat aaccgcaatc agggacgtac
     3721 agataaaatc gataacgcct ggggtgataa atttgagttt atagttttct ataacataat
     3781 taataatgct accaacagca ctgataatta tggtgtagat taaaaattgc accgcatgga
     3841 ataaaaaacc ttttctttta cgatttccgc ggcgaaacca aaagctcatc agaatcaatg
     3901 ctgcgcagct accgaaaata atgaccagat taagcgcatt atttgtaaac agttcagcga
     3961 tcattgtttt atcaggctcc tccagataat tgtcgtcatg ccggaacccc ctggcggggc
     4021 tattttaccg cgacaattca ttcagatcat caatagtcag ggaaggaagt agcaacatta
     4081 g
//
LOCUS       DADLFA010000246         4063 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000246 DADLFA010000000
VERSION     DADLFA010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.248"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..1046)
                     /locus_tag="JQH65_003833"
     CDS             complement(<1..1046)
                     /locus_tag="JQH65_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HAY6141086.1"
                     /translation="MREINMLRNISSCLFPHISKITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
     gene            1537..1647
                     /locus_tag="JQH65_003834"
     CDS             1537..1647
                     /locus_tag="JQH65_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HAY6141087.1"
                     /translation="MLIGYVRVSTNDQNTDLRRNALNCAGYELIVERMGA"
     gene            1927..2922
                     /gene="pgl"
                     /locus_tag="JQH65_003835"
     CDS             1927..2922
                     /gene="pgl"
                     /locus_tag="JQH65_003835"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HAY6141088.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTIEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHLISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(2963..3915)
                     /locus_tag="JQH65_003836"
                     /pseudo
     CDS             complement(2963..3915)
                     /locus_tag="JQH65_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT         1087 a          829 c          951 g         1196 t
ORIGIN      
        1 aatgcccggg aattctcctc ggtggaaaaa gaagtccatt gtgtagaaac ctcccgacga
       61 tctgcttcag gaaaccaggc tgccacagtt tcgggaagag gggggatctg aaagttctgc
      121 cccccttcag tgatgactct gatatggcgc gaatgtaaat tttgcggcaa catctggcgt
      181 aaaaacagtc tgttttggtc tgacagggaa atacgaaact gaggcccgtt aaaatttgga
      241 accgaagttt gctgtattaa gaatagcaga gtttgatctg ataaggggtt atttgcaatc
      301 tcaatagtta tatgaggatc taaacgaaaa agatttgtcg ggattgtagt tatttggttg
      361 ttttcaaaat aaaaaaatct tctcatgata atattttctt ggatgtttgg taagttgctt
      421 aatctattgc tatatgcaaa aagaagttgc agtctcctag gtaaaggggg cagttcagta
      481 agatggttgt gcgcaacctt aagtatctta agattagaag gcagagatgg aagagtttgc
      541 agttcgttgt tcctaacctc aagttttttt aaattcaagg gtaatgaagg cagtgtggat
      601 aacctattat actctgcgct tagtgatttg atggtgtctg gtaataacgg cagattctct
      661 aattgatttt cggacacatc caaagttatt agataatgtg gtaatgcaga tatatgtgtt
      721 aagaaattat ctcttgcaat aagttcttta atattttcag gtaaatcagt aaggatggtt
      781 aatttattac agctcacatc aagcttttta atgcaagcag ggagtacagg taacgaggaa
      841 agcgccaagg ctcgcaggtc cagtttatgc cccttagagg taagacatgc acgaagtctt
      901 cttaccgcct cagtacgctg ttcttccggt aacccctgtt tctcccaatc atcccattcg
      961 gacaaataat ggttggggga tgtaattttg ctgatatgtg gaaataaaca ggatgaaata
     1021 tttctgagca tattaatttc tcgcatatgg atatggaatt agattgtgaa taaattgtat
     1081 ttaatgtatc tctcctaatg aggagggtgt ttcagaatat tcgccataag ggaatgagtg
     1141 ttatatcatg ctttctatcc tggtaagtat atttgctttt ttcgtatatt tggttcatct
     1201 ttttttgatg ttttacatat tcacaatgca gaggatttat accagaagac attagtcttg
     1261 agtaagatac tacaacatct ccaactattt tagccagcct gactacttga cttggtaata
     1321 tttgttgcta ataatcatgt ataaaataag cttatataca tagtaacttc agcaaaggat
     1381 ttagtaatga tccaaatcat atatcaatgt actgtttttt tctttaaaaa aacagtacat
     1441 tctattttat aatgactgtg tattcccatg gaaggagatc acccaattct cccccccgac
     1501 ttttgacgca gtgtcttgtt aacaggggga tttaccatgc ttattggcta tgtacgcgta
     1561 tcaacaaatg accagaacac cgatctacga cgtaatgcgt tgaactgtgc aggatatgag
     1621 ctgattgttg agcgaatggg agcttgattg gaagttgtta gggtatggtg tttacggtcg
     1681 ttgatatggc gaacaaacgc ggggagcgtc acgctcccta aatggtcgcg aaaatatctg
     1741 tggcattgtc aaatttgtga cggtgactaa tattttaatg atatgccatt cgaaatggtg
     1801 tgctaacgta ttgatttaat tgtgacgcga tgttgtattt tatcgcgttt tgttgtctgt
     1861 ttttcattcg cttgatgttg ctatttacat gaaaaataaa gaatttattg tttttgaggt
     1921 gtgtttatga agcaaacagt ttatatcgcc agccctgaga gccagcaaat tcacgtctgg
     1981 aacctgaatc atgaaggcgc actgacgctg acacaggttg tcgatgtgcc ggggcaggtg
     2041 cagccgatgg tggtcagccc ggacaaacgt tatctctatg ttggtgttcg ccctgagttt
     2101 cgcgtcctgg cgtatcgtat tgccccggac gatggcgcac tgacctttgc cgcagagtct
     2161 gcgctgccgg gtagtccgac gcatatttcc accgatcacc aggggcagtt tgtctttgta
     2221 ggttcttaca atgcgggtaa cgtgagcgta acgcgtctgg aagatggcct gccagtgggc
     2281 gtcgtcgatg tggtcgaggg gctggacggt tgccattccg ccaatatctc accggacaac
     2341 cgtacgctgt gggttccggc attaaagcag gatcgcattt gcctgtttac ggtcagcgat
     2401 gatggtcatc tggtggcgca ggaccctgcg gaagtgacta ccattgaagg ggccggcccg
     2461 cgtcatatgg tattccatcc aaacgaacaa tatgcgtatt gcgtcaatga gttaaacagc
     2521 tcagtggatg tctgggaact gaaagatccg cacggtaata tcgaatgtgt ccagacgctg
     2581 gatatgatgc cggaaaactt ctccgacacc cgttgggcgg ctgatattca tatcaccccg
     2641 gatggtcgcc atttatacgc ctgcgaccgt accgccagcc tgattaccgt tttcagcgtt
     2701 tcggaagatg gcagtgtgtt gagtaaagaa ggcttccagc caacggaaac ccagccgcgc
     2761 ggcttcaatg ttgatcacag cggcaagtat ctgattgccg ccgggcaaaa atctcacctc
     2821 atctcggtat acgaaattgt tggcgagcag ggactactgc atgaaaaagg ccgttatgcg
     2881 gtcgggcagg gaccaatgtg ggtggtggtt aacgcacact aaccgctgat ttaaccggcg
     2941 cagtctctcc tgcgccggtg tattaaccta tctcctgtaa cgcatgtctc tggcgttcga
     3001 cgatattggt ccacaaattg tctttatcgt cagtccacaa attaatcagc aaggcaaaaa
     3061 agcgttcagc tgccggagaa agcacggcat ctttgcggcg aataatcccc aatgtccgac
     3121 gtataacggg ttccaccagt gggataccaa taatcgaaga atagggcgca tggggcattg
     3181 ccagaccagg aagcgccgaa atccccagtc ctgcctccac tagtcctaat gacgtcgaaa
     3241 gatgacgcac ttcgtaaaac cagtccagct tccagggctt gtcggccagc tgttgttcta
     3301 tcaacagtcg gttgccgctg gaggagcgta cgccaatcat tttgtaaccc actaactcct
     3361 gccattctac aagctgcttt ttggccaatg gatggtcacg ccgacaggca gcacgaacgg
     3421 ctcgttgacc agtggggtaa aatcaatgga tgaatttgtg acgttgttca tgtttatgcc
     3481 aaagtctgat tcgttacaca gcaccgattc catgcaatta ttcgtgcctt gttccagaat
     3541 ccgcacttta atattgggat acagctcatt aaatttaccg attgccagcg gtaaaaaata
     3601 aaatactgcg gtcggaatac acgctaacgt caccatacca cgatgataag cattcatatc
     3661 acgaatatta aaaagcgttt catcgaattt ctttattaat tcccgcgcct ccgggagcaa
     3721 cctttttcct gcttttgtta acgtaacctt gcgagttgta cgctcaaata gttgaatatg
     3781 taaatcttcc tccatctttt ttattcttcg cgttaatgca ggttgcgtaa tattgagtaa
     3841 tttagcggca ttgttaaacg aactggactc tgccagtatg acaaatgcct tcatacttga
     3901 taattcatgc ttcattatta ctccggaaaa tggaagcgac gattttgggt ggctggccgt
     3961 taaaaatttt aactgcattt agccaactta aattaatgaa aaaatgttat taatcgttga
     4021 gctaaagtca ttagagatgc tttgccctta atgtaaccat atc
//
LOCUS       DADLFA010000247         3850 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000247 DADLFA010000000
VERSION     DADLFA010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.249"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..439
                     /locus_tag="JQH65_003837"
     CDS             <1..439
                     /locus_tag="JQH65_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAY6141089.1"
                     /translation="FTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVR
                     ISIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIA
                     EGVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            447..1775
                     /locus_tag="JQH65_003838"
     CDS             447..1775
                     /locus_tag="JQH65_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY6141090.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGSYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKIYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(1822..3147)
                     /gene="rimO"
                     /locus_tag="JQH65_003839"
     CDS             complement(1822..3147)
                     /gene="rimO"
                     /locus_tag="JQH65_003839"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HAY6141091.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            3360..3743
                     /gene="bssR"
                     /locus_tag="JQH65_003840"
     CDS             3360..3743
                     /gene="bssR"
                     /locus_tag="JQH65_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HAY6141092.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
BASE COUNT         1006 a          857 c          949 g         1038 t
ORIGIN      
        1 ttttaccacc atcatgaaag aaaatggcct gaaggcgaac agcctctgtg ttgagatcac
       61 tgaaaccgtg atcgagcgaa ttaatgaaca tttttatctc aatattgaac aactgcgtaa
      121 acaaggggta cggatatcga ttgatgactt tggcaccggt ttgtcaaacc tgaaacgttt
      181 ttatgaaatt aatccagaca gcataaaggt ggactcgcaa tttaccggcg atattttcgg
      241 tactgcggga aaaattgtgc gcattatttt cgatctggca cgctataacc ggatcccggt
      301 gattgcggaa ggcgtagaga gcgaagacgt tgcacgcgaa ttaattaaat taggatgtgt
      361 tcaggctcag gggtatctgt accagaaacc tatgccattc tccgcctggg ataaaagtgg
      421 aaaattagta aaagagtagt ttacgtatgt ccagaatcaa taagttcgta cttacagtca
      481 gtctgctgat ttttatcatg atttcagcag ttgcctgcgg gatctacact caaatggtaa
      541 aggaacgggt gtatagcctg aaacagtccg ttattgatac tgcttttgcg gtggcaaata
      601 ttgctgaata tcggcgtagc gtggcaattg atcttatcaa cacgctaaat cccactgagg
      661 aacagctgtt ggttggtttg cgcacagctt acgccgactc ggtttccccc tcttatttgt
      721 acgatgtcgg tccttatctg atttccagtg acgaatgtat tcaggtaaag gagttcgaga
      781 aaaattattg tgcagatatt atgcaggttg tgaagtatcg acatgtcaaa aatacagggt
      841 ttatctcttt tgacggtaaa accttcgtct attacctcta tccggtaact cacaatcgta
      901 gtctgatatt tttgcttggt ctggagcgtt tttctttact gtcaaaatcg ctggcgatgg
      961 acagcgagaa cctgatgttc tctctattta agaacggtaa accggtgacc ggtgatgaat
     1021 ataatgctaa aaacgccatc ttcaccgttt cggaagcgat ggagcacttc gcctatttgc
     1081 cgaccggatt gtacgtattt gcgtataaaa aagatgttta tttgcgggtc tgtacattga
     1141 ttattttctt tgccgcattg gtggctgtga tatcgggtgc cagttgcctc tatctggtac
     1201 gcagagtgat taatcgtggt attgtggaga aagaagccat cattaataac cattttgaac
     1261 gcgtactgga tggcgggctt ttcttttcgg ctgccgatgt caaaaaactc tacagtatgt
     1321 ataactcggc gttcctggac gacctgacca aagcaatggg cagaaaatcc tttgacgaag
     1381 atttaaaagc gctgccggaa aaaggcagtt atttgtgcct gtttgacgtc gataaattca
     1441 aaaacattaa cgacaccttc ggtcatttgc tgggcgatga agtgttgatg aaagtggtga
     1501 aaatccttaa atcacagatc ccggtagata aaggtaaaat ctaccgcttc ggcggtgacg
     1561 aatttgcggt gatttatacg ggtggaacgc tggaagagtt gctatcgatt ctaaaagaaa
     1621 tcgttcattt ccaggtggga agcattaatt taagtaccag tatcggtgta gcgcattcaa
     1681 atgaatgtac caccgtcgaa cgcttgaaaa tgctggcgga tgagcggctg tataagagta
     1741 agaaaaacgg cagggctcag attagctggc agtaatcatt attcgcaggc cggacaaatg
     1801 attttgcccg gcctgaatta attaaacccg gctaccccac aaatcgtact catcggcgtg
     1861 ctcgactttc acacgcagga tatcacccgg cttaacgttg gtttcaccat tgagataaac
     1921 cgcgccgtcg atttccggtg catctgccat gctgcgacca atcgcgcctt cttcgtccac
     1981 ttcgtcgata atcaccagaa tttcacggcc cactttctct tgcaggcgct cggcggaaat
     2041 ctgctgctgc agctgcatga aacggttcca gcgttcttct ttaacttctt ccggaacctg
     2101 gtcaggcagg gcattggcgt ctgcaccttc aaccgggctg tatttaaagc agccaacgcg
     2161 atccagacgc gcttctttca ggaagtcgag tagcatctgg aaatcttctt ctgtctcgcc
     2221 agggaagccg acaataaagg ttgagcgtag ggtcagttcc gggcagattt cgcgccactg
     2281 tttgatgcgc gccagttggc gatctacaga acccggacgc ttcatcagtt tgagaatgcg
     2341 cgggctggcg tgctgcaacg gaatgtccag atacggcagg attttgcctt ctgccatcag
     2401 tgggatgacg tcgtccacat gcggataagg gtaaacgtag tgcagacgtg tccagatccc
     2461 cagtttcgat aactgttcgc acaggctgac catgctggtt tttaccggct cgccgttgtg
     2521 gaagccagta cgatgtttaa catcaacgcc ataggcggaa gtatcctgcg agatcaccag
     2581 aatctcttta acgcccgcat ctaccagacg tttcgcttca cttaacactt cgccaatcgg
     2641 acggctcacc aggtcgccgc gcatagacgg aataatgcag aaggtgcagc ggtgattaca
     2701 gccttccgaa attttcagat aggcataatg acgcggcgtc agtttcacac cttgttctgg
     2761 caccaggctc aggaatgggt tgtgtttcgg ttttggcacg tagtgatgaa cgtgctccag
     2821 aacctgctca tagctatgag gcccggtgat ttccagcact ttcgggtgga cttcgcggat
     2881 ctgatcttct tttgccccca gacaaccggt cacaattacc ttgccgtttt cattcaacgc
     2941 ttcaccaatg gcttccagtg attcttgtac cgcgctgtca ataaagccgc aggtgttgac
     3001 gatcaccatg tccgcatcgt catagctcgg taccacgtca taaccttcag tgcggagttc
     3061 ggtgagaata cgctctgaat cgacaaggtt tttcggacag ccaagggaaa caaagccgat
     3121 tttcggctgg ggagttactt tgctcatagc ttaaaaaata ttcagttaca ggaaaggtca
     3181 gggcagggat tctacagagt tctggataaa atttgtatcg caatctcatt cgctggcgga
     3241 ggcgaaggaa atgtaaattt tgttaattcg gcgtgaagaa ttgatcctgg acagcatttt
     3301 gctcaaaaaa tagccatact atttaattgc aacaaggctg gaaagaggag gatcgaagta
     3361 tgttcgttga cagacagcga atcgatctgc tgaaccggtt gatcgacgca cgcgttgacc
     3421 tcgctgcata cgtgcaactg aggaaggcaa aaggatacat gtccgtcagc gaaagcaatc
     3481 atctacgaga taactttttt aaactgaatc gcgaactgca cgataaatcg ctgcggttga
     3541 atcttcatct ggatcaggaa gagtggagtg ctcttcatca tgctgaagaa gcattagcga
     3601 cagccgcagt atgtttgatg agtgggcacc atgattgccc gactgttatt accgtcaacg
     3661 ccgataagct tgaaaattgt ctgatgagct taacgctgag tatccagagc ctgcagaagc
     3721 acgccatgct tgagaaggcc tgaaaactaa gggggagaaa gcgtctcccc cttcatgttt
     3781 aagtttttgt aaaaatgaat ttgttatctc ctccactgac tacgctttaa gccagagtca
     3841 atccggaggc
//
LOCUS       DADLFA010000248         3817 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000248 DADLFA010000000
VERSION     DADLFA010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.250"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            17..253
                     /locus_tag="JQH65_003841"
     CDS             17..253
                     /locus_tag="JQH65_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004982904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C TorY domain protein"
                     /protein_id="HAY6141093.1"
                     /translation="MNPGTDLTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALA
                     TLSEEGIKALTLNGEWQAGEFSASNMVNRNTGCW"
     gene            193..1575
                     /locus_tag="JQH65_003842"
                     /pseudo
     CDS             193..1575
                     /locus_tag="JQH65_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(1740..2711)
                     /gene="cmoB"
                     /locus_tag="JQH65_003843"
     CDS             complement(1740..2711)
                     /gene="cmoB"
                     /locus_tag="JQH65_003843"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HAY6141094.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRVVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2708..3451)
                     /gene="cmoA"
                     /locus_tag="JQH65_003844"
     CDS             complement(2708..3451)
                     /gene="cmoA"
                     /locus_tag="JQH65_003844"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HAY6141095.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MISMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            complement(3492..>3817)
                     /locus_tag="JQH65_003845"
     CDS             complement(3492..>3817)
                     /locus_tag="JQH65_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141096.1"
                     /translation="RLQYRVAYGDGGFSELQSAIRIHGNAVEYIPIAIVLMLFMEMNG
                     AETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRSGMSATWCALLLMVLANLWYMPWEL
                     VFSLR"
BASE COUNT         1009 a          952 c         1008 g          848 t
ORIGIN      
        1 aggcgagctg gctaccgtga atcctggtac cgatctcacc gtcgttgatg ccagtggcaa
       61 acagccgatc gttctgttgc agggttatca aatgcagggc agtgaaaaca cgctctacct
      121 ggcggcaggt caacggctgg cgctagccac attaagtgaa gaaggtatca aggcgctcac
      181 gctaaacggg gaatggcagg ctggggaatt cagcgccagc aatatggtga acagaaacac
      241 tggatgctgg tgacgctggc ggcggtgtta gggcagattg gtacaccggg cggcggtttt
      301 ggtttctctt atcattactc caatggtggc aacccgacac gtgttggcgg cgttctgccg
      361 gaaatgtctg cggcgattgc cggacaggcc agcgaggccg ctgatgatgg cggaatgagg
      421 gcgattcctg ttgcccgtat cgtcgatgcg ctggaaaatc ctggcggcaa atatcagcat
      481 aacggtaaag agcaaacgta tccgaatatc aaaatgatct ggtgggcggg tggcggtaac
      541 tttacccatc accaggatac taatcgtctg attaaagcat ggcagaaacc ggagatgatc
      601 gtcgtttctg aatgctactg gaccgcagct gctaaacatg cagatatcgt attaccgatc
      661 accacatcgt ttgagcgcaa tgacttgacg atgaccggtg attacagcaa ccagcatatt
      721 gtgccgatga agcaggctgt cgctccgcaa tttgaagcgc gaaacgattt tgatgtgttt
      781 gccgatcttg cggaattact gaaacctggc ggaaaagaga tctataccga aggtaaagat
      841 gaaatggcgt ggctgaaatt tttctatgat gccgctcaga aaggtgcccg tgcgcaacgc
      901 gtgactatgc cgatgtttaa tgccttctgg cagcaaaata aactgatcga aatgcgccgc
      961 agcgagaaga acgaacagta cgttcgttat gctgatttcc gcgccgatcc ggtgaaaaat
     1021 gcgctgggta cgccaagcgg caaaattgag atttactcca aaacgctgga aaaatttggc
     1081 tataaggatt gcccggcaca tccaacctgg cttgcgcctg atgagtggaa gggtaccgcc
     1141 gacgagaagc agttgcagct tctgaccgca catccggcac accgtttaca tagtcagctt
     1201 aactatgcgg aactgcgtaa aaaatatgcg gttgcagatc gtgaaccaat cactattcat
     1261 accgaagatg ctgctcgctt tggtattgcg aatggtgatc tggtgcgtgt gtggaacaaa
     1321 cgtggtcaga ttctgacagg cgcggtggtg actgacggga tcaaaaaagg cgtggtatgc
     1381 gtgcatgaag gtgcatggcc agatctggaa aatggcttgt gtaaaaacgg cagtgcgaac
     1441 gtgttaacgg cggatatccc cagctcgcag ctggcaaatg cctgtgccgg taactctgcg
     1501 ctggtgtata tcgaaaaata tacgggtaac gcaccgaagt taacggcatt tgataagcct
     1561 gctgttcagg cgtaagaagt gaatagcgcc cggaaacggg cgctgaggtt atgcaaacgt
     1621 aaaactgcct gatgcgctat gcttatcagg cctacatagt ccctgcaata gattgaatta
     1681 gcaagggttt gtaggccgga taaggcattc acgctgcatc cggcagtaat accagacctt
     1741 tacggcttgc gcgcaatcag caccgcgcgt ttaggcgcag gataaccttc aactgtctta
     1801 ctcggatcat gtgggtcgag gaaatcggac agagattcgg taaccatcca ttcagtgcgt
     1861 cgctgctctt cagtagtggt aacgcacaca tccacaacgc ggatatctac aaaaccacac
     1921 ttcttcagcc agtttttcag cgccagcgcg gaagggatga aatagacatt acgcatttgc
     1981 gcgtaacgat cgcctggtac cagcaccgtg ttctcatcgc catcaataac cagcgtttcc
     2041 agcaccagtt caccctcatt caccagttga tctttcaact gccagagatg ctccagcggc
     2101 gaacggcgat ggtaaagcac gcccatcgaa aagacggtat caaaggcttt cagtgccgga
     2161 agctgttcaa tacctaacgg taacaaatgt gcgcgctgat cgttacccag tagtttacgc
     2221 accgcttcaa actggcacag aaacagttgc gtagggtcga tccccaccgc gaggtgcgcg
     2281 cctgcgccaa tcatgcgcca catgtgataa ccgctgccgc agccgacatc cagaatggtg
     2341 cgtccggtta aatcagaaag atggggcaga acacgatccc atttccagtc ggaacgccat
     2401 tcggtatcga tgttgacgcc atacagtgag aacggccctt tgcgccatgg catcaggttc
     2461 cgcataagcg tttcaatgcg tttaatttgc ccggtgctca gtggctcttc gctttcggcg
     2521 gttacgctat gcaataaatc cagacgatac ggtttaattt caggcagaaa ttccaccgcg
     2581 ttggaccact gcttaaacag cccgtgctgc tgctcgcgct gccagttagc aatctgcgcg
     2641 ggcagcgttt cgagccagtg tgaaagatga tttttggcaa tcagagaata aaagttacca
     2701 aagtcgatca tgcagcgtcc tctgctttta atgccaccag tgaaccaaag ttaaagcact
     2761 ggaaccacag ctcgctatgc tcaaaaccgg ctttatgcag gcgtgcttta tgggtttcca
     2821 cggaatcggt cagcatcacg ttttccagca tgctgcgctt ctggctgatc tccagttcgc
     2881 tgtaaccgtt ggcacgttta aagtcgtggt gcatgttgaa caacagttca ccaactttgg
     2941 catcttcgaa actgaatttt tccgaaagca ccagcgcgcc gcccgggttc agtccttgat
     3001 aaattttatc cagtaatgcc tggcgctcgg aaggttccag gaattgcagg gtaaaattca
     3061 gcaccaccat cgatgcgttt tcaatggcga tatcgcgaat atcaccttca atgacgtcta
     3121 ctggcgtagg ggctttatag gcgtcaatat gacgacggca gcgttcaatc atcgccgggg
     3181 agttgtcgat ggcaataatt ttgcaattat catgatgaat gttgcgacgc accgagagcg
     3241 tcgccgcgcc cagagaacaa cccagatcgt aaacctgcgt accaggttga acgaagcgct
     3301 cggctaacat actaatcatg gaaataatat tggaatagcc gggaacggaa cgctggatca
     3361 tatccgggaa gacttcagct acccgttcat caaaggtcca gtcgcccagt ctggcgatag
     3421 gggcagaaaa tagcgtgtcg cggtgagaca taacgtaaaa atccgggaaa aagaaagtgg
     3481 cgtattgtgc gctaacgcag ggagaaaacc aactcccagg gcatatacca aagattcgcc
     3541 agcaccatca gcaacagcgc acaccaggtg gcgctcatgc cggaacgtcg ccagcgaaac
     3601 agacggtgat gaaaaccgta ataatgcatc agacgaccag caagcaaaac gatgccgcaa
     3661 atatgcacca tccaggtttc tgcgccattc atttccataa acagcatcag cacaatcgcg
     3721 atgggaatat attccaccgc gttaccatga atgcgaatag cgctttgcag ttcgctaaaa
     3781 ccaccgtcac cataggcaac gcggtactgc aaacgca
//
LOCUS       DADLFA010000249         3804 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000249 DADLFA010000000
VERSION     DADLFA010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.251"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..>3804
                     /locus_tag="JQH65_003846"
     CDS             <1..>3804
                     /locus_tag="JQH65_003846"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAY6141097.1"
                     /translation="GTDVQRNLQLPLKGIADERRRRGETVIIDEVHAVAGSKRGAHLA
                     LSLERLDALLHTSAQRIGLSATVRSASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPV
                     ANMDDVSSVASSTGEDSHAGREGSIWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTA
                     RLNELYAARLQRSPSIAVDAAHFESTSGATSNRVQSSDVFIARSHHGSVSKEQRAITE
                     QALKSGELRCVVATSSLELGIDMGAVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKG
                     LFFPRTRRDLVDSAVIVECMFAGRLENLTPPHNPLDVLAQQTVAAAAMEALQVDEWYS
                     RVRRAAPWKDLPRRVFDATLDMLSGRYPSGDFSAFRPKLVWNRETGILTVRPGAQLLA
                     VTSGGTIPDRGMYSVLLPEGEEKAGSRRVGELDEEMVYESRVNDIITLGATSWRIQQI
                     TRDQVIVTPAPGRSARLPFWRGEGNGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAE
                     ENTNANIQGLIDEQRNATGIVPGSRHLVLERCRDEIGDWRIILHSPYGRRVHEPWALA
                     IAGRIHALWGADASVVASDDGIVARIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSA
                     LFAARFRECAARALLMPGRTPGHRTPLWQQRLRASQLLEIAQGYPDFPVILEILRECL
                     QDVYDLPALERLMRRLNGGEIQISDVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERR
                     ASVLSLDSELLRNLLGQVDPGELLDPQVIRQVEEELQRLAPGRRAKGEEGLFDLLREL
                     GPMTVEDLAQRHTGSSEEVASYLENLLAVKRIFPAMISGQERLACMDDAARLRDALGV
                     RLPESLPEIYLHRVSYPLRDLFLRYLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQ
                     GLVMNLQQDIWVSDEVFRRLRLRSLQAAREATRPVAATTYARLLLERQGVLPATDGSP
                     ALFASTSPGVYEGVDGVMRVIEQLAGVGLPASLWESQILPARVRDYSSEMLDELLATG
                     AVIWSGQKKLGEDDGLVALHLQEYAAESFTPAEADQANRSALQQAIVAVLADGGAWFA
                     QQISQRIRDKIGESVDLSALQEALWALVWQGVITSDIWAPLRALTRSSSNARTSTRRS
                     HRARRGRPVYAQPISPLVSYNTPNLAGRWSLLQVEPLNDTERMLALAENMLDRYGIIS
                     RQAVIAENIPGGFPSMQTLCRSMEDSGRIMRGRFVEGLGGAQFAERLTIDRWRDLATQ
                     ATQTR"
BASE COUNT          794 a         1041 c         1115 g          854 t
ORIGIN      
        1 tgggcaccga cgttcagcgc aacttgcagc tcccgttgaa gggtattgcc gatgaacggc
       61 ggcggcgcgg cgaaacggta attattgatg aagtccacgc ggtagcgggc agtaaacgtg
      121 gtgcgcatct ggcgttaagt ctggagcggc tcgatgcgct gctccacacc tcagcacagc
      181 gaattggcct ttctgccact gtgcgctcag ccagcgatgt ggcagccttt cttggtggcg
      241 atcgcccggt tacggtagtc aacccgcccg caatgcgcca tccgcagata cgaattgtcg
      301 taccggtcgc caatatggat gatgtctcat cggtcgccag cagcaccggc gaagacagcc
      361 atgccggccg ggaaggctcc atctggccat atattgaaac gggtatcctt gatgaagtgt
      421 tgcgccatcg ctcgaccatt gtctttacta attcacgtgg gctggcggaa aaactgacgg
      481 cacgattaaa tgagctttac gccgcacgct tacagcgttc cccgtctatc gccgttgatg
      541 cggcccattt cgagtcgacc tccggcgcaa cctctaaccg tgtacaaagt agcgacgttt
      601 ttattgcccg ctcacaccac ggctccgtct ctaaagaaca acgagcaatc accgagcagg
      661 cgctgaaatc gggtgagtta cgctgcgtgg tcgcaacctc cagtctcgaa ctggggattg
      721 atatgggcgc agtggatctg gtgattcagg tggcaacgcc gctttctgtt gccagtgggt
      781 tacaacgcat tggtcgcgcc ggacatcagg ttggcggtgt atctaaaggg ctgtttttcc
      841 ctcgtacccg gcgtgactta gtcgattccg cagtcattgt agagtgtatg ttcgcaggca
      901 ggctggaaaa cctgacaccg ccgcataatc cactcgacgt ccttgcgcag caaaccgttg
      961 ccgccgcggc gatggaggca ttacaggtag acgaatggta ctcccgcgta cgccgtgccg
     1021 caccgtggaa agatctgcca agacgtgttt ttgacgccac gctggatatg ctttccgggc
     1081 gctatccctc tggcgatttt tctgcttttc gccccaaact ggtctggaac agggagaccg
     1141 ggatattgac cgtccgacct ggcgctcaat tgttggcggt taccagcggc ggcaccattc
     1201 cggatcgtgg catgtatagc gtgttattac ccgaaggtga agaaaaggcc ggttcgcggc
     1261 gggtgggtga actggatgag gagatggtat atgagtcgcg ggtgaacgac attatcactc
     1321 tcggcgctac ctcatggcgg atccagcaaa tcacccgcga tcaggtgatt gtgactcctg
     1381 ctccgggtcg ttctgcccgg ctccccttct ggcgtggtga aggtaacgga cgtccggctg
     1441 aattaggcga gatgatcggc gattttcttc atttactggc ggatggcgcg ttcttttccg
     1501 ggactattcc cccgtggctg gcagaagaaa atacgaacgc caatattcag gggttgattg
     1561 acgagcaacg caacgcgacg ggcatcgttc cggggagtcg ccatctggtc ctcgaacggt
     1621 gccgtgatga aattggcgac tggcgtatta ttttgcactc tccctatgga agacgggtgc
     1681 atgaaccctg ggcgctggcg attgccgggc gaatacatgc cctatggggc gctgacgcgt
     1741 ctgtggtcgc cagtgatgac ggcattgttg cgcgcattcc tgacactgat ggtaaattgc
     1801 ccgacgccgc gatttttttg tttgaaccag aaaagttgct gcaaattgtc cgcgaggcgg
     1861 taggcagctc ggcacttttc gccgcccgtt ttcgcgaatg cgccgcgcgg gcattattaa
     1921 tgccggggcg cactccgggc catcgcaccc cgctttggca acaacggctg cgcgccagtc
     1981 agttgctgga aatcgctcag ggatatccag attttccggt cattctcgaa atcctacgcg
     2041 aatgtctgca agatgtttat gatcttcccg cactggaacg tttgatgcgt cgcctgaacg
     2101 gtggcgaaat tcaaatatcc gatgtaacga ccactacgcc ctcgcctttc gccacaagtt
     2161 tattgttcgg ctatgtcgcg gaatttatgt accagagcga cgccccgctg gcagagcgcc
     2221 gggcatccgt actgtcgctg gacagcgagt tactgcgcaa tctactcgga caggtcgatc
     2281 cgggggaatt actcgacccg caggtcattc gccaggtgga agaagagttg caacgactgg
     2341 ctcctggcag aagagcgaaa ggtgaagaag gattgttcga cctgctgcgc gaactggggc
     2401 caatgaccgt tgaagacctg gcgcaacggc atacaggcag cagtgaagag gttgcgtcgt
     2461 atctggaaaa tcttcttgca gtaaaacgaa tcttcccagc gatgattagc ggacaggagc
     2521 gtcttgcctg tatggatgat gccgccaggc tgcgtgatgc cctcggcgta cgactaccag
     2581 agtcattgcc agagatttat ttacatagag tcagttaccc gcttcgcgac ctctttctgc
     2641 gctatctccg ggctcatgct ctggtcacgg ctgaacaact ggctcatgag tttagtctcg
     2701 gtattgccat tgtcgaagag cagcttcagc aactgcgtga acagggtctg gtgatgaatc
     2761 tgcaacaaga catctgggtg agcgatgaag tatttcgtcg tctgcgtttg cgctcgctgc
     2821 aagccgccag agaagcgacg cgtcccgttg cagccacgac ctatgcgcga ttgctgctgg
     2881 aacgtcaggg cgtattaccc gccaccgatg gtagcccggc gctctttgcc tcaacatcgc
     2941 caggcgttta tgagggcgta gatggcgtga tgcgggtgat cgaacagctt gccggagtcg
     3001 gtttacccgc ctcactctgg gaaagccaga tcctgcctgc ccgcgtacgc gactattcgt
     3061 cagaaatgct cgatgaatta ctggcaaccg gtgcggttat ctggtcgggg caaaaaaaac
     3121 tgggtgaaga tgacggcctg gtggcactgc atctacagga atatgctgca gaatcgttca
     3181 ctcccgccga agcggatcag gcgaatcgtt cggcgctgca acaagcgata gtcgctgttc
     3241 tggctgacgg aggagcctgg tttgcacaac aaatcagcca acggatacgc gacaaaatcg
     3301 gcgaatcggt tgatctctct gccctgcaag aggcgttatg ggcgctggtc tggcaaggcg
     3361 tcatcaccag cgacatttgg gcaccgttac gcgccctcac ccgcagcagt tccaacgcac
     3421 gcacctcaac tcgccgcagt caccgggctc gtcgtggacg tcctgtctat gcgcaaccca
     3481 tctcgccgct ggtatcttac aacacaccaa atctggctgg acgctggtcg ttattgcagg
     3541 tggagccact aaacgatacc gaaaggatgc tggcgctggc ggaaaatatg ctcgaccgct
     3601 acggcatcat cagtcgtcag gcggtgatag ccgaaaatat ccctggcggg tttccatcga
     3661 tgcaaacgct ttgtcgaagt atggaagact ccgggcgaat tatgcgaggt cgttttgtag
     3721 aaggtctggg tggcgcgcaa ttcgctgaac gtctgactat tgaccgatgg cgcgatctgg
     3781 cgacacaagc cacgcaaacg cgcc
//
LOCUS       DADLFA010000250         3782 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000250 DADLFA010000000
VERSION     DADLFA010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.252"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..210)
                     /locus_tag="JQH65_003847"
     CDS             complement(<1..210)
                     /locus_tag="JQH65_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HAY6141098.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAAKAELKLIAEKVKKGYVEQAKD"
     gene            complement(351..2384)
                     /gene="metG"
                     /locus_tag="JQH65_003848"
     CDS             complement(351..2384)
                     /gene="metG"
                     /locus_tag="JQH65_003848"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAY6141099.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQESRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMRQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2516..3625
                     /gene="apbC"
                     /locus_tag="JQH65_003849"
     CDS             2516..3625
                     /gene="apbC"
                     /locus_tag="JQH65_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HAY6141100.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHCLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
BASE COUNT          906 a          970 c          970 g          936 t
ORIGIN      
        1 atccttcgct tgctccacat accccttctt caccttctcc gcaatcagct taagctccgc
       61 ttttgccgct gccgcagcat ctgaaaaact ttttatctgg ctttgccctt tggtgccaac
      121 ttttccccaa ctgatatgca actcgtttcc ctgttgctca actgcccaga atttatgtga
      181 tttttcgtcc tgatagataa agtgtctcat tctgcatccc tgtaatttta tccagttgaa
      241 tgcagatgct accagtattt atgcgggtta gagagagaca aaatcgcaag aaaacaactt
      301 taggaattta taaagaaaag gcgctgtcga tgcagcgcct tgaaggggga ttatttcacc
      361 tgatgacccg gtttagcacc ggcatccggg cttagcagga aaatatcttt cccgccagga
      421 ccggcagcca tcaccatgcc ttcagagata ccgaagcgca ttttacgtgg tgctaggtta
      481 gccaccataa tggtgtgacg accaatcagt gcctgcggat ccgggtaagc agaacgaata
      541 ccggagaaga cattgcgttt ttcaccgccg agatccagcg tcaggcgcag cagtttgtca
      601 gaaccttcaa caaactctgc gttttcaatc agcgccacgc gcaggtcaac tttagcgaag
      661 tcgtcaaagg tgatggtttc ctgaatcgga tcatctgcca gcgggccagt taccggcgcg
      721 gcagcggctt ttacttcttc tttagaggct tccaccagtg cttcaacctg cctcatatcg
      781 atgcggttat acagcgcctt gaacggattc actttgtggc ccagcagcgg ttgctggata
      841 ccatcccagg tcagttccgt attgaggaat gcttctgcac gctcggtcag tttcggcagt
      901 accggcttca ggtaagtcat cagcacgcgg aacaggttga tgcccattga gcaaattgcc
      961 tgcaggtcgg catcgcggct ttcctgtttc gccaccaccc acggagcctg ttcatcgaca
     1021 tagcggttag ccagatcagc cagcgccatg atttcgcgca cggctttacc aaattcacgg
     1081 ctttcccacg cttcaccaat cacttcagcg gcatcagtga aggttttgta caactgcggg
     1141 tcagccagtt cgcttgccag cacgccgtca aaacgcttgt tgataaagcc cgcattacgg
     1201 gaggccaggt taaccacttt gttaacgata tcggcattca cacgctgaac gaaatcttcc
     1261 aggttgagat cgatatcatc gatgcgcgaa gagagtttcg cagtgtagta gtaacgcagg
     1321 ctgtctgcgt caaaatgatt cagccaggtg ctggctttaa taaaggtgcc gcgagacttg
     1381 gacatcttcg cgccgttcac cgtcacatag ccatgaacaa acaggttggt cggcttgcgg
     1441 aagttgctgc cttccagcat ggcaggccag aacaggctgt ggaagtaaac gatatcttta
     1501 ccgatgaagt ggtacagctc ggcggtggag tctttcttcc agtattcatc gaagcttacg
     1561 ctgtcgccgc gcttgtcgca cagattcttg aaagaaccca tgtagccaat cggtgcgtcc
     1621 agccagacgt agaaatattt gcccggcgcg ttcggaattt caaaaccgaa gtaaggtgcg
     1681 tcgcgggaga tatcccactg ttgcaggcca gattcaaacc actcctgcat tttgtttgcc
     1741 acctgctcct gcaacgcacc gctgcgggtc catgcctgca acatttcgct gaaagagggc
     1801 agatcaaaga agaagtgttc agaatcacgc attaccgggg tagcgccaga aaccaccgat
     1861 ttcggctcga tcagttcagt cgggctgtag gtcgcgccgc agacttcgca gttatcgccg
     1921 tattgatccg gggatttaca tttcgggcag gtgcctttca caaaacggtc cggcaggaac
     1981 atgccttttt ccggatcgta cagctgagag atggtgcggt ttttaataaa accgttttct
     2041 ttcaggcgag agtagataag ttctgacaac tggcggttct cttcgctgtg tgtcgagtga
     2101 tagttgtcat agctgatgtt aaagcctgcg aaatcagtct gatgctcctg actcatttcg
     2161 ccaatcatct gctccggggt gataccaagc tgctgagctt tcagcatgat cggtgtaccg
     2221 tgggcatcgt cggcgcagat gaagttgacc tcgtggccgc gcattcgctg gtaacggacc
     2281 cagacatcag cctggatgtg ctccagcata tggccgaggt ggattgagcc gttagcgtac
     2341 ggcagtgcgc acgtcaccag aattttcttc gcgacttgag tcatagtagg cattacttct
     2401 taatagtgaa aaggggttag atagtaccaa atgggaaaat gttaagtaag tacttctcta
     2461 tattcttcag gtaaactatc agctgatggt ttatctatac aaaaaaggag tcgggatgaa
     2521 cgaacaatcc caggccaaat caccagaagc cttgcgcgca atggtcgccg ggacactggc
     2581 caattttcag cacccaaccc tgaagcataa cctcaccacg ctgaaagcgt tgcaccatgt
     2641 cgcctggatg gacgacacgt tgcatgtgga actggttatg ccattcgtct ggcatagcgc
     2701 gtttgaagag ttaaaagagc aatgtagcgc cgaactgctg cgtatcaccg gggcgaaagc
     2761 tatcgactgg aaactgtcgc acaatatcgc caccttaaaa cgcgtgaaga atcagccggg
     2821 tattaatggt gtgaagaaca tcattgcggt cagttccggt aaaggcggcg tgggtaaatc
     2881 ctccacggcg gtaaacctgg cgctggcact ggcggcggaa ggggcgaaag tcggcattct
     2941 ggacgccgat atctacggcc catcaattcc caccatgctg ggcgcagaaa atcagcgtcc
     3001 aacttcgccg gacggtaccc atatggcacc gattatgtct cattgcctgg caaccaattc
     3061 aattggttat ctggtcactg acgacaatgc gatggtgtgg cgtggaccga tggcgagcaa
     3121 ggcgttaatg cagatgttgc aggaaacctt gtggccggat ctcgactatc tggtgcttga
     3181 tatgccgcca ggcaccggtg acattcaatt gacgctggcg caaaacattc cagtaactgg
     3241 tgcagtcgtg gtcaccaccc cgcaggacat cgcgttgatc gatgcgaaaa aaggcattgt
     3301 gatgttcgag aaagtcgagg ttccggtgct tggtattgtc gagaacatga gtgtgcatat
     3361 ttgcagtaac tgtggtcacc atgaaccgat tttcggtacc ggtggtgcag agaaactggc
     3421 tgaaaaatac cacacacagc tgctggggca gatgccgtta cacatctcct tacgtgagga
     3481 tctcgataaa ggcacgccaa cggttatcag ccgtccggag agcgaattta ccgctatcta
     3541 ccgccagctg gctgaccgcg ttgcagctca gctctactgg cagggtgaag tcattccagg
     3601 cgagatttcc ttccgcgcgg tctaacgcct attaaccatt ctggttattc ttcatacacc
     3661 gcagagatgt tacattgatg cggtgtatta gtttttgccg cattaatgaa cacttattta
     3721 atactgcgaa tattctgcgg accttcgtcc gaaactctac aaagtcgatt gatcatacag
     3781 gc
//
LOCUS       DADLFA010000251         3737 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000251 DADLFA010000000
VERSION     DADLFA010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.253"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..860)
                     /locus_tag="JQH65_003850"
     CDS             complement(<1..860)
                     /locus_tag="JQH65_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAY6141101.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESAIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAE"
     gene            complement(885..1763)
                     /gene="yihU"
                     /locus_tag="JQH65_003851"
     CDS             complement(885..1763)
                     /gene="yihU"
                     /locus_tag="JQH65_003851"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HAY6141102.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTQAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGTPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            1936..2832
                     /locus_tag="JQH65_003852"
     CDS             1936..2832
                     /locus_tag="JQH65_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAY6141103.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYNLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTKEAIRFASAVAAMKCT
                     QPGGRAGIPNREQTESFLSLYA"
     gene            2872..3660
                     /locus_tag="JQH65_003853"
     CDS             2872..3660
                     /locus_tag="JQH65_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY6141104.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDLNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
BASE COUNT          962 a          955 c          902 g          918 t
ORIGIN      
        1 atttctgcca accgttgtaa acgaggaaca gagacatcgc gcagcatagt ttgtggatct
       61 tgtgcaccaa caaccgacgc ccaaaccgct cgtccagcca ggaagcccga cgcaccacct
      121 ttcatggcaa tacttactgc acggccaaaa atatcggcat caacgccaga agaaagaatt
      181 acccacggca tcttcatctg atcgttaagt tgctgacagg cggcaagtaa ggttttttca
      241 tcgcctttgc cccctaatgg catctcggct ttgtaaagat cagcctctgt tccaccaagc
      301 tctgcggcgg cggcaacaat ggcgctttca cgatcaaagt cccaaccgcg acgaggtgga
      361 cgcaccacag gttcaataat gctcaccagc ccggcactac ggcagcgacg aacaaattta
      421 tcaaccattg ccagacgttc ctctgcgggt tcatcttcac gccagagtac aagtaatttc
      481 attgccttag cccccatttc acgggcttta tgcgggtcga cggacatatc tatttcaacg
      541 ctatcaaccg ggatgccatt acctgggata aaaagatcgg cagcgacaat caggccgcaa
      601 gaattagcaa cagcgccctg ctcgactatt tgctcaagac aaaattgttt atccgccaga
      661 acggcagaag cataaggaga aaggattcgg gtcgcggcaa ctttaaaatc ggtgagtaca
      721 ctgtcggcga tgggttttgg ctgccctgct gccgcaaaca tcaagcgcat ggcctcacgc
      781 tgatcgacag ctaacatggc aaatccacca cctgggcggg agatatcttt cagcgtaaat
      841 ggggtgaagt gggatgacat agaaactcct gttaatagat aaaattaaag accagcctgc
      901 tggcgaacct gatttagcag ggcactccag tcctgccgcc ctttgccgtc ttgacttgct
      961 gcagcgtaat aactttctgc tgcaatcccc atattgagcg gtgtccccgt ctcccgagcg
     1021 acgtcaactg caatacgcag atctttcaac gccagatcca ccatgaaggc gggtgaaaga
     1081 tcgcctttca gcactttgcc aggccaggtg gtcgtaaaat gtcctttccc tgctgccgta
     1141 ccactcatca ccttaattgc gacatcaaga tttaatccca gtgactcaca taacacagcg
     1201 gcctcggagg acagcgcatt aagcgcaatg ctcatatagt tgttaattag cttcacgcgg
     1261 atccccattc ctgggccgcc agcctcgacc agctcattac ccatgcacat aagaatcggg
     1321 cgtgcacgtg caatctgctc atcagtgcca cccgctaaaa tcagcaatgt gccatcaatc
     1381 gcattcacgg acgttctgcc cacgggagcg tccatcatac tgatgccttt ttcttgtaac
     1441 tcccgtatta atgcatcggt ctgtaaggga tgaatagtcg acatatcaat caataacgcg
     1501 tcagatgaaa cagcctcaca gacgcctttt tcccccaaca gaacctgtcg aacaatgtca
     1561 ccattgggaa gcatagtgat gataaattca gcgcctgctg ctgcttccgc ttgggtttgt
     1621 gccgcagtgg ccccctgcgt aactagcgca tcgacggcct gtgcattgac atcaaatacc
     1681 tgcaatgaat gccctttttg caatagattg ttagccattg ggctacccat ctgccccagc
     1741 ccgagaaatg caatggttgc catgaatact ctcctgtctg acattgacaa aataatgaca
     1801 attgaaaatt cgtttttgtc tgtttttgag catatttgtg atttatgttc aaaaacacga
     1861 cacgcatcac taatttgatc atttgcgtca tttaaattga ccaaagacga caaaattaaa
     1921 caggacaaga caaatatgac cagaattgca tgcgtgggta tcacagttca ggacagaatt
     1981 tattctttgc ccactctgcc ggagggcggc gggaaatatc aggcaaatca ttatcttgag
     2041 attggtggcg gcccggcagc aacagccgct gttgcaatcg caaagcttgg cgtggaggtt
     2101 gatttcatcg gtcgcgtcgg cgatgacagt tgtggaaata ccttacttgc agaacttgag
     2161 ggttggggcg tcaataccgc attttgccgc cgatatccta acgcccgttc gtcgcaatca
     2221 gcgatcctcg ttgatcagca tggcgaacgg attatcgtta attatccaag tcctgatctc
     2281 ggtactgatg ccgaatggtt agaagcgatc gatttctctc gttataatct gattctggcc
     2341 gatgttcgct ggcatagcgg tactgaaaaa gcattctcgc tagcgcgcct tgcgggtgtg
     2401 acaacgcttt tagacgccga tatgacaccg caggatattt ctccacttgt cgcgcttgcc
     2461 gatcacgccg tgttctcaac accagggctg aaacgcatga ctggcctgca gtctcctgaa
     2521 gaaggtttgt tccaggcaac gacacaaaca gcaggtaaag tgtatgttac gctgggttct
     2581 gaaggatcgt tgtggattga agatggtcat ttgtgtcaac aggaggcatt ttctgtcaat
     2641 gtggttgata ccactggagc aggcgatgtt tttcacggcg cactcgcggt agcgttggca
     2701 gaaaaaatgc caacaaaaga ggctattcgt tttgccagtg cggttgccgc gatgaaatgt
     2761 acccagcctg gcggtcgggc cggcattcct aatcgtgagc aaaccgaatc atttttgtca
     2821 ctttatgcgt aaaatggtaa acatctaccg aggaggggca agggagtatt catgagtatc
     2881 atcgaagtga caggtaatcc acgccatgac caactcgttc atctaatcgc agaacgcggt
     2941 tatatgaata ttgaagaact ggcacaactg ctggatgttt caacgcaaac cgtgcgccgg
     3001 gatatccgaa aactcagtga gcagggcttg atcacccgtc atcatggagg ggccgggcgc
     3061 gtctccagcg tcatgaatac cgcttttgag caacgggaac tttcgctcac cgccgagaaa
     3121 cgggcgatcg ctgaggcagt cgccgattac cttcccgaac gctgtaccgt ctttatcacc
     3181 atcggtacaa ccgtagaagc cgttgccagg gcattactca accggcgtga tttacgcatt
     3241 atcactaaca gcctacgtgt ggcacagatt ctttataaga atcaggatat tgaagtgatg
     3301 gtgccgggag gcactttacg cgctcataac ggcgggatta tcggcccagg agccgtggat
     3361 tttattgaag gcttccgcgc agattattta atcaccagta taggtgccat tgaacatgac
     3421 ggcaccctac tggaatttga tctaaatgaa gcgttagtcg cgagaacgat gattaaacat
     3481 gcgcggaata cattgttagt ggccgatcat acgaagtttg ccgcgtctgc tgccgtttca
     3541 attggcaatg cacggaatgt cagggctttt tttactgatg ccccgcctcc caattctttc
     3601 tgccagttgt taagtgaaga gaatgttgaa ctggtggttg ccgagcaaga agtatcctga
     3661 aattgaaatt tcggaaaatc cgctgggaat ttcagcggat tttgttttcc ataagcaaaa
     3721 aaaacctgcc agcgatg
//
LOCUS       DADLFA010000252         3728 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000252 DADLFA010000000
VERSION     DADLFA010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.254"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(118..2187)
                     /gene="glyS"
                     /locus_tag="JQH65_003854"
     CDS             complement(118..2187)
                     /gene="glyS"
                     /locus_tag="JQH65_003854"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAY6141105.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLVESFAANFTVELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYACHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2197..3108)
                     /gene="glyQ"
                     /locus_tag="JQH65_003855"
     CDS             complement(2197..3108)
                     /gene="glyQ"
                     /locus_tag="JQH65_003855"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAY6141106.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3203..3502)
                     /locus_tag="JQH65_003856"
     CDS             complement(3203..3502)
                     /locus_tag="JQH65_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141107.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
BASE COUNT          864 a         1006 c          958 g          900 t
ORIGIN      
        1 cataaaatta atatatgaga taatctttat tcagcagaag attattaaag gttgctgtat
       61 tatttagcga taataaaaaa agcctgccag atggcaggct ttttaataac ggcgttatta
      121 ctgcaacagc gaaatatccg caacgcgcag gaacagttcg cgcagtttct ccagcatggt
      181 cagacggttg agacgcaatt ctttgtcatc aaccatgacc atcactttat cgaagaaagc
      241 atcaaccggc tcacgcagct ctgccagttc gaccagcgca tcctggtaac gaccttccgc
      301 aaagtacggc tccagcttgt cacgtagcac cacaacctgc atcgccagtt taatttcttc
      361 cggctctttc agggtagagg cattcacgcg gtcgctcagc acttcgtcag atttcgccag
      421 aatgttagat acacgtttgt tcgccgccgc cagtgcagca gctgcatcca gggtacggaa
      481 atgcgatacc gctttcatac gggcatcgaa atcagccgga cgagtcggac gacgcgccag
      541 taccgcctgg atggtgtcaa cggtgtaacc ttcgtcctga taccaggcgc ggaagcgacc
      601 gagcataaag tcgataacat catcaaccac gttggcgtta gtcagcttat cgccatacag
      661 acgcactgct tcttcggtca gcgtttgcag atcaaggttg aggttcttct caacgataat
      721 tcgcagcacg ccaagtgcgg cacgacgcag cgcaaacggg tctttgtcgc ctttcggatg
      781 ctgaccgata ccgaagatac ccgccagagt atccatctta tcagcaatcg ccagcgcaca
      841 agctaccggg ttggacggca ggtcatcacc ggcaaagcgc ggctgatact gctcattcag
      901 cgcgacggct acgtcttccg cttcgccatc gtgacacgcg tagtgcatcc ccataacgcc
      961 ttgggtgtcg gtgaactcga agaccatgtt ggtcatcagg tcacacttag acagcaggcc
     1021 cgcacgggtt gcgtggttaa cgtcagcgcc aatctgttca gcaatccagc cagccagcgc
     1081 ctggatgcgg tcagttttgt cacgcagcgt acccagctgt tgctggaaca gcacggtttg
     1141 caggcgcggc aggttatctt ccagacgttt tttacggtcg gtattgaaga agaactcggc
     1201 atccgccaga cgtggacgaa cgactttctc gttaccagag ataatttgct gcggatcttt
     1261 cgattcgatg ttggcaacga agatgaagtt cggcagcagt ttgccatcgt tcgcatacac
     1321 cgggaagtat ttctggtcac ctttcatggt gtaaaccagg gcttcagccg gcaccgcgag
     1381 gaatttctct tcgaattttg cggtcagaac gaccggccac tccaccagcg aagccacttc
     1441 ttccagcagg ctttcgctta agtcagcgtt accgccaatc ttacgcgctg cttcttcggc
     1501 atcggcttta atcttcgctt tacgttcttc gtaatcggcg atgactttcc cgcgctcacg
     1561 cagaatttcc ggatactgat cggcgttgtc gatggtgaat tccggttcgc ccataaagcg
     1621 gtggccgcga atcacgcgat cggactgaat gcccagaata gttgccggaa tgactttgtc
     1681 gcccagcagc agggtcactg tgtgcaccgg acgcacgaag tgcacgtcgc ttgcgcccca
     1741 acgcatcagt ttcgggatcg gcagtttcgc cagagaagtc gcaaccatat tcggcagcag
     1801 tgcttcggtg ctttcgccct tcacatgggc gcgatacagc agccattcgc ctttatcggt
     1861 agtcagacgc tcagcctggt caacggtaat accgcaacca cgcgcccaac cttctgccgc
     1921 tttgctcggt ttgccttcag cgtcgaacgc ctgggcaatc gccgggccgc gtttttcgat
     1981 ttcacgatcc ggttgcgctt ccgccaggtt agctactttc agcgccagac gacgcggagc
     2041 agcaaaccat tgaacggtgc cgtgtgcgag gccagcgtta tccagctcca cagtaaaatt
     2101 cgcagcaaag gactcaacca ggctgcgcag tgcttttggt ggcagctctt cagtgccgat
     2161 ttccaccaga aaagttttct cagacatagc cgcctcttac ttatctttgt tgcacatcgg
     2221 gaagccgagg gcttcacggg aagcgtagta tgcttctgcc actgctttgg tcagggtgcg
     2281 aatgcgcaga atgtagcgct gacgctcggt gacggagatg gctttacgcg catccagcag
     2341 gttgaagctg tgggcggctt tcagaatacg ctcgtaggct ggcagcggca gcggattttc
     2401 cagcgccagc agctgctgcg cttctttctc gtactgctca aagcaggtga acaggaagtc
     2461 cacatccgcg tattcgaagt tgtaagtgga ctgctccact tcgttctgat ggaacacgtc
     2521 gccgtaggtg gttttaccca gcgggccgtc gctccagacc aggtcgtaaa cgctgtctac
     2581 gccctgaatg tacatggcca gacgttccag accgtaggtg atctcgccgg taaccggttt
     2641 acactccaga ccaccaacct gctggaagta agtgaactgc gtcacttcca tgccgtttag
     2701 ccacacttcc cagcccagtc cccaggcacc cagagtcggg ttttcccagt tatcttccac
     2761 gaaacggatg tcgtgaatag tcgggtccat gcccagctct ttcagagaac cgaggtacag
     2821 ctcctgaata ttgtccggcg atggcttaat gaccacctgg aactgatagt agtgctgtaa
     2881 acggttgggg ttttcgccgt agcgaccatc ggtcgggcga cgagaaggct gaacataagc
     2941 agccgccatc ggttctggcc ccagcgcgcg cagacaggtc attgggtgag aggttcccgc
     3001 gccgacttcc atgtccaatg gttgaacaat ggtgcagccc tggcgagccc agtaatcctg
     3061 taaggtcagg atcaagccct ggaaggtcct ggtatcaaac ttttgcatat tatttcgtgc
     3121 tggatacgtg tggatttaaa ggaagggatc agtatacccg ctggatggaa gatatacagt
     3181 acgaaacggg aaaaagcagg gcttaacgca tggaaagatg caaaaaatgg ccatctgcgt
     3241 caaaagaaca gacaaactgc tctttacgga aggtataacc gcgcatttca tagcttccct
     3301 ggaactgttc gaaggcggtg acgtcgattt tttgctcgcc ggtgttataa cgttttgccg
     3361 cctgatcctt gcataacgct tccatattca acgaacgcag aggatcaacc tttacccgtt
     3421 gtgctttctg cacgggtgac ggtgtagaac aacccactag caccataagc gccattgccg
     3481 gaaagaaagc gttcatcatc attaaattac cgtctggttt gcaggtgagt cttattattt
     3541 atatcggtat agtggctgat atcttacgaa tttccttaag cctctataag ctgtagtgat
     3601 aaaactcaga ttattccagt gcgccaacca aatgatttat cggtcagact ttttttaacc
     3661 agatacagag acacagatgc aggtaatgct gccaacttac tgatttagtg tatgatggtg
     3721 attttaag
//
LOCUS       DADLFA010000253         3721 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000253 DADLFA010000000
VERSION     DADLFA010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3721
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.255"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            35..262
                     /locus_tag="JQH65_003857"
     CDS             35..262
                     /locus_tag="JQH65_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HAY6141108.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(263..637)
                     /locus_tag="JQH65_003858"
     CDS             complement(263..637)
                     /locus_tag="JQH65_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HAY6141109.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(720..1944)
                     /gene="mdtG"
                     /locus_tag="JQH65_003859"
                     /pseudo
     CDS             complement(720..1944)
                     /gene="mdtG"
                     /locus_tag="JQH65_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
     gene            complement(2116..3036)
                     /locus_tag="JQH65_003860"
     CDS             complement(2116..3036)
                     /locus_tag="JQH65_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family Kdo(2)-lipid IV(A)
                     lauroyl/palmitoleoyl acyltransferasee"
                     /protein_id="HAY6141110.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3261..3721
                     /locus_tag="JQH65_003861"
                     /pseudo
     CDS             3261..3721
                     /locus_tag="JQH65_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415573.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
BASE COUNT          915 a          951 c          944 g          911 t
ORIGIN      
        1 ttatgccggt atttttttac ggaagaggta tcaaatgcgt ttaattgtgg tgagcatcat
       61 ggtgaccttg ctgagtggct gtggcagcat tattagtcgc actataccgg ggcaggggca
      121 tggcaaccaa tattatcccg gtgtgcaatg ggatgtgcgt gactccgcct ggcgttatgt
      181 cacaatcctt gatctgccat tctccctggt ttttgatact ttactgctgc cgatcgacat
      241 tcatcatggc ccgtatgagt gattaacgtt catcccactc atcagctgct gcgcgtcctt
      301 cctcggtatc cagcggtggc tcaagctgaa attccccctc gtcccattca tgtaatgtat
      361 tctcttcctg ccactcctgg cgtatctcta tctcatcata gtcgccatca aaaacacttt
      421 gcgcggcttc accgctaagc ataggtaaac attcaccttc ttccccttcg tcggcaaaaa
      481 actcaacttg ccacatgatg tcgccgtcct gcaaaacgta tttttgggca ttgaactgtt
      541 gcacattcgc atcttcggcg tcgatgccgg ggttgtctgc aagaaattct tcgcgtgcag
      601 cgtcaatggc ttcttcaagc gttgcgtaca tggtcatcac gattctccct ttgagttgat
      661 gaggtttcag ggaaaaggat agctgattct ccgcttttgc aagtatgaaa ggcgaaaaat
      721 cagttcgata cctggggtat tcgacgacga cgtagactgt tccatgaata gactgcgttg
      781 aataacacta cgccagcggt gacgagaaat accgctctga aaccgtagtt cgctgaaatc
      841 gctgctccca tcaatggtcc ggtaacgttg ccaatatcac gaaacgattg gttatagctg
      901 aagatacgcc cggcgatctg gttgctcgag ttgtaaacca acaatgtctg tacggcgggg
      961 agtagtgcac catcggcggc accgagcaaa aaacgtaaaa tcccaagttg caatggcgtc
     1021 tgaacgtaag acattgggat caacagcagt acagaaaaga tcagcgctgt aatcaggatc
     1081 ttttcgggtc cgattcgatc gccaagtttg ccgagtcgtg gtgcacttag cagagccgcc
     1141 acgcctggca ccgaggcgat catgccactg ataaaggcga cgttactgac gttacccgcc
     1201 agttcgcgga catacagcgt cagaatgggg gcaattgagc ccgtcgccac ctggatgatt
     1261 aacgtagtga caaacaggct gagtaccagt ttcgggtttt taagtgatgt caccacttcc
     1321 cgcatgtgca gcatctcttt tttgctgacc ggctggaatt tttctctgat gcaaaacagg
     1381 gtgacgaaaa agcagagtat gagcacactg gtggtaataa agaataccgg acgtaagccg
     1441 tagctatcgg cgagcaggcc gccagccatt gggccgagca acgcaccacc aacgccgcct
     1501 gtggagagcg tacccagcgc ccagccgctt ttattacgcg gtacttgtgt ggcgataaga
     1561 gcattagcgt tggggacaaa tccgccaagt aacccaagaa gcgcccgcag gatcaaaaac
     1621 tgccagatat tttgtgccag ccccatcaac accatcacga tgcccatgcc gagggcagag
     1681 cgtaatagca tgagttttcg gcctttacgg tcggcgagtc caccccaaaa cggtgaggcg
     1741 atggccgaaa ataaaaatgt aatgctgaag acaataccgg accatattca gggcggagtg
     1801 accggtaacg ccaagctgct caacgtagag gggtaagaag ggcattacca gactgaaggc
     1861 ggcaccggta agaaaacagc ctagccaggc gacgatcagg tttcgtttcc agtttatagg
     1921 ggtgtcattt tcacagggtg acatagcaat ccgctgttgg tgcgccaggc gcggtgaaca
     1981 taagaagaaa agataagcac actaattatg cgcccgactt ccagggggcg caatccagag
     2041 agcttttatc gctaaatcag ggggatttgc tgtggtaatg ccggatgcca ttctgaagca
     2101 tccggcaggg gagatttaat agcgtgaagg aacgccttcc gggcgtgttt taaagcgacg
     2161 gtgtaaccac atatactgct ctggtgccat catgatgcat ttttcgacca ctttgttcat
     2221 ccacgcggcg gtagtttcgg catcatccag tggcggagaa cactctggcg gcagcataat
     2281 caattgatac cctttgccat ctggcttacg gcgtggaacg aagggcacca gacatgcgcc
     2341 ggacatccgt gccagcatcc aggttccggt cgtggtcgca gcctgctcaa cggcaaataa
     2401 cgggacgaaa acgcttgagc gcgggccgta atcatgatcc ggtgcgtacc agaccacttc
     2461 gccttttttc agggctttaa tcatgccttt taaatctttg cggtcgagca tcgatttatt
     2521 tgagcgcaaa cggccccagg tttgtagcca gtcaatcagt ggattatcgt tcgggcggta
     2581 aacgccaatc cccggttcct gcataccaaa ctgccgcgca cccagctcca gtgtcagaaa
     2641 atggatgcca actaacagga tgccgcgttt ttgcgcctgc acgtcacgaa tgtgttccat
     2701 gccgatcact tccgtccagc gcgcgattcg gcggtccggc cagaaccacg ccatgccggt
     2761 ttccatcagg cccatgccaa cggattcgaa attcttcacc accattttac ggcgttcttg
     2821 ttcgctcatt tccgggaagc acagttccag gttgcgatgc acaatttttg cgcgtcgttt
     2881 cataaaacgt aacgccagtt ttcctaatcc acaaccgagg cggtagataa ccgggtaggg
     2941 caattgcacg actaaccaaa gtacgccaat acccaaccag gttaaccaat aacgcggatg
     3001 aagcagtgcg gtggagaact tgggtagatt cgtcatatca atcctgtttt tcaacctatt
     3061 cgggcaattg tatgtattgt cgcatttttt cgcccgcaac caaaatttgt ggctgaagac
     3121 tgggcgaaat tgccgcgctt gtaaataaca aataattttt aatgcgcaaa tgtagcgtaa
     3181 aatgtgtgga tgttaattat cgataattgc tatatcatgc cgcggatttt tactttccca
     3241 tctcgcagga accgtacacc atgccagtgt tacacaaccg catttccaac gacgcgctaa
     3301 aagccaaaat gttggctgag agcgaaccgc gaaccaccat ttcgttttac aagtatttcc
     3361 acatcgccga tcctaaggcg acccgtgacg ctttatatca gctgtttacc gcgctgaatg
     3421 tttttgggcg agtgtatctg gcgcatgagg gcattaacgc gcaaatcagc gtacctgcga
     3481 gcaatgttga aacatttcgc gcgcagctct gccttcgacc cggctttaga gggtttacgc
     3541 ctgaatatcg cgttggatga tgacgggaaa tccttctggg tactgcgcat gaaggtacgt
     3601 gatcgcattg ttgccgacgg tattgacgat cctcactttg atgccagcaa tgttggtgag
     3661 tatctgcaag cggcggaagt gaacgccatg cttgacgatc ccgatgcact atttatcgac
     3721 a
//
LOCUS       DADLFA010000254         3685 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000254 DADLFA010000000
VERSION     DADLFA010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.256"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(279..566)
                     /locus_tag="JQH65_003862"
     CDS             complement(279..566)
                     /locus_tag="JQH65_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141111.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            complement(641..784)
                     /gene="mgrB"
                     /locus_tag="JQH65_003863"
     CDS             complement(641..784)
                     /gene="mgrB"
                     /locus_tag="JQH65_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HAY6141112.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            943..1182
                     /locus_tag="JQH65_003864"
     CDS             943..1182
                     /locus_tag="JQH65_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141113.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(1325..2116)
                     /gene="kdgR"
                     /locus_tag="JQH65_003865"
     CDS             complement(1325..2116)
                     /gene="kdgR"
                     /locus_tag="JQH65_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HAY6141114.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            2293..3666
                     /locus_tag="JQH65_003866"
     CDS             2293..3666
                     /locus_tag="JQH65_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY6141115.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
BASE COUNT          924 a          904 c          815 g         1042 t
ORIGIN      
        1 taaaataaga aaaattgatg aatgagcaaa aaaatcaaga gagaaacgtt tcgctaataa
       61 ataataccac gcttaatcac aaatccgatg taaacatcct aataaattaa tgaggaaaat
      121 atctttttct gatatcccgt tacgcaaata aataatatga taattatttg aagtcagacc
      181 agagttttaa tttgaagcag ttgcaccatg acagtgcgta taatcaggat gaattgagta
      241 acccttcaac gacggggtta ctcaagattg aggaaggatt atcgttcggc gaccaggcga
      301 ataatatctt cgtcatcaac cgatttttga ctctcaatgg tctttttatc tgctttctct
      361 ggttcgtttg cttcgcacaa acgacgtaaa agtgcatttt gacgtttttg ctgatccagc
      421 aacgcctcaa gcagttcaat ctgttcgtta gtccgtgaac tggcacgatt gataaaaaac
      481 cacaagatga gcccgataag aagaactacc accgatacaa ccaaagacgc aatgttcatc
      541 acgcctgaat ttacaacttc gttcatttca ccacctctga gtagaggcgc tattctacca
      601 ctgctggaga ggaagaaaat ctagtgctga aaaaatgata tcaccacggg ataaactggt
      661 taatggcaca aattccgctg aaaaattgta catcctgatc gcacatcatg ttgaatacct
      721 gcgcccaaag cagcaagcaa gccaacacca cgacaaccag aacgacccat cgaaactttt
      781 tcactccaat ctccgcttca cctaccttat gtcattaaac tagcatgcgt tagttaaaca
      841 gcgcctaact atgtcgatat tcgtgctttt tcggaatgag tagcttgctt cagcgcatat
      901 tgccgctacg attaagcgaa cataaaaaga gaagaggttg taatgcgatt catcattcgc
      961 acagttatgc tgatagcact ggtatggata ggattattac tcagcggata tggcgttttg
     1021 attggaagca aagagaatgc cgcaggatta ggtttgcaat gtacctatct gactgccaga
     1081 ggcaccagca cggtacaata cttgcacact aaaagtggtt tcttggggat aacagattgc
     1141 ccactgttac gtaaaagcaa tatcgtggtc gataatggtt aagggtaata aataaccaga
     1201 ttctcactca tgagtaaaat attcttatag caagcgcgtt taatataacg caccttcaac
     1261 tttatttttc tcatgctaaa taaagaacct gtctgtttga tattccgaca ggttcttact
     1321 ctcatcagaa cggatagtcg tgataaccca tttgggcaga aattttgcgc gctgcggtat
     1381 gcaacattgc gacatattcc tgtaaacgct cttcagagaa acgcaacgtc gggaaggaga
     1441 tgctcaaacc ggcaatgacc acgccaaagc gatcaaatac cggtaccgca atgcatcgca
     1501 gcccttcttc ctgctcttcg ttatcttcgc catacccctg ctcgcgcacc tggtccagaa
     1561 cgggtaataa cgcttctgta ctggtgatgg tccgctcggt actgcgttta tactccacgc
     1621 cctcaagaat ttgcttcact tcatcgcgat cgcgccatgc cagcagtacc ttaccaatcg
     1681 cggtgctgta cagcggatta cgacgcccaa tccgtgaata catgcgcaaa ttgtacatag
     1741 agtcaatttt gtgaatgtaa acaatactgt cttcgtccag tgcgccgagg tggatagttt
     1801 ctttggtcag gcgggagatc tcacgcatct ggatatctgc gctacgaatt aaatcgacgt
     1861 tttgtaacgc gcgagcgccc agttcaaaca atttcagggt cagggaatat ttctccgact
     1921 ccccttcctg cgccacataa cctaaggttt tcatggtctg taaaaagcga taaacggtgc
     1981 tttttgacat catgacgcgc tgcgacagct cggttatccc tatttcgcgc tcttcaccca
     2041 gcgcctgcaa aatgccaaaa acttttagca cggaagatac agaatcaggc tgtttatcca
     2101 gatctgcgtt agccatttat cacctcattg cgagtgtttt ataaaaatca gaactgtttt
     2161 ttattataat ttcgcaccag gggggtcgca atccatcttt tgccggttag ttacaattct
     2221 gcgacatcca ccgtgaatat cagtgctaga atcatacccc tgttgattat tcaccaaaga
     2281 tataaaattc ctatgccaaa agttcaggcc gacggcctgc cattgcccca gcgatacggt
     2341 gcgatattaa ccattgtgat tggtatttcg atggctgtcc ttgacggcgc aatcgccaac
     2401 gtcgccctgc caacaatcgc cacggacctt catgccacgc cagccagttc catctgggta
     2461 gtgaacgcct atcaaatcgc cattgtcatc tccctgctct cgttttcgtt tctgggcgat
     2521 atgtttggct atcgacgtat ttataaatgc ggtctggtcg tttttctgtt gtcttcactg
     2581 ttctgcgccc tttctgattc gctgcaaatg ctcacccttg cgcgtgtcat acaaggtttc
     2641 ggcggtgcag cgttgatgag cgttaatacc gcacttatcc gcctgatcta tccacaacgt
     2701 tttctgggga gagggatggg cataaactcg tttattgttg ccgtctcttc tgctgccggg
     2761 ccgacaattg ctgcagcaat cctctccatc gcatcctgga aatggttatt tttaatcaac
     2821 gtaccgttag gtattatcgc cctgcttctg gcgatgcgtt ttctgccacc caatggttct
     2881 cgcgccagta aaccccgttt cgacctgccc agcgccgtga tgaacgcgtt aaccttcggc
     2941 ctgcttatca ctgcgttgag tggtttcgct caggggcaat cgctgacgtt aattgctgcg
     3001 gaactggtgg taatggttgt tgttggtatt ttctttattc gccgccagct ttctcttccc
     3061 gtaccgctgc taccggtgga tttactgcgt atcccgctgt tttcactttc tatttgcaca
     3121 tctgtttgct ctttctgcgc acaaatgctg gcaatggttt ccctgccctt ttacctgcaa
     3181 accgtgctcg ggcgtagtga agtcgaaaca ggtttacttc tgacaccgtg gccgttagca
     3241 acgatggtga tggctccgtt ggcaggctat ttgattgaac gcgtacatgc aggattgctg
     3301 ggtgctctag ggttgttcat catggctgcg gggctttttt ccctggttct gctgcccgcg
     3361 tcacctgcgg atatcaatat tatctggccg atgatcttat gtggcgctgg atttggcttg
     3421 ttccagtcac ccaataacca caccattatt acctccgctc ctcgcgaacg tagcggtgga
     3481 gccagtggca tgttaggaac ggctcgtcta ctgggtcaga gtagcggcgc ggcactggtg
     3541 gcgctgatgc taaatcagtt cggtgataat ggtacgcacg tttcgctgat ggctgcggct
     3601 attctggcag tgattgctgc ctgtgtcagt ggtttacgta tcactcagcc acgatccagg
     3661 gcataaaaaa agcgcgtcga tcagg
//
LOCUS       DADLFA010000255         3677 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000255 DADLFA010000000
VERSION     DADLFA010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.257"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(167..481)
                     /gene="fliE"
                     /locus_tag="JQH65_003867"
     CDS             complement(167..481)
                     /gene="fliE"
                     /locus_tag="JQH65_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HAY6141116.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQDSLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            696..2354
                     /gene="fliF"
                     /locus_tag="JQH65_003868"
     CDS             696..2354
                     /gene="fliF"
                     /locus_tag="JQH65_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body M-ring protein FliF"
                     /protein_id="HAY6141117.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     LSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTSRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            2347..3342
                     /gene="fliG"
                     /locus_tag="JQH65_003869"
     CDS             2347..3342
                     /gene="fliG"
                     /locus_tag="JQH65_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HAY6141118.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            3335..>3677
                     /locus_tag="JQH65_003870"
     CDS             3335..>3677
                     /locus_tag="JQH65_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HAY6141119.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAKAKSQQAPIHARMQQLV
                     SEFQTTLDALDS"
BASE COUNT          925 a          966 c         1029 g          757 t
ORIGIN      
        1 ttcctatgag caaagcgaag acaaaacatg ttatcgcgtt tacggatcgt ggatggcaga
       61 aaactttcag gatcaggtat ccattttaaa ccagaaatag ggtgagtttg tcccatccat
      121 gccccacgcg ggcagatggg atgtaattac caacaggtta caaaacctac acctgcatgg
      181 acatcacttc ctgatacgcc gccaccagct tattacgcac ctgaatcccc atttgcatag
      241 aaactgaggc tttttgcata tcggtcatca catcgtttaa cgccacgccg ggttcaccga
      301 gggtgaattt ttctgcctgc gtgcgggcgg ctgtttgtgt atcgcttatg cgatcgagcg
      361 cggcgtgcag ctgcccggca aaactaatgg tcggttgcgg cagtgaatcc tgcgcacgcg
      421 cactcatcgc cgtagcctgt aactggctga taaccccttc aatcccctgt atcgctgaca
      481 ttttcgtctc ctggataatt tctggtagca aagcctacca gtaagtcaat aagacaaagg
      541 cgctaaatag caacaaaaaa acgggtttat tggcggatag aaaaaaacga aagcacaaat
      601 aatggcagcg tcaatttttc gagtttgctg acccgggagt gagtcttgtt ccactttgcc
      661 aataacgccg tccataatca gccacgaggt gcgcgatgaa tgcgactgca gcccagacaa
      721 aatctcttga gtggcttaat cgcctgcgtg cgaatccgaa aattccattg attgttgccg
      781 gttccgcggc agtggcggtc atggtcgcac tgatcctgtg ggcgaaagcc cccgactacc
      841 gcacattatt cagcaatctt tccgatcagg atggtggcgc aattgtcagc caactgacgc
      901 aaatgaatat tccttaccgc ttcagcgaag ccagcggcgc tattgaagtt ccggcagata
      961 aagttcacga gctgcgtctg cgcctggcgc aacaaggttt gcccaaaggc ggcgcggtcg
     1021 gtttcgaact actcgatcag gaaaagtttg gtatcagcca gttcagcgaa caggtgaatt
     1081 atcagcgggc gctggaaggc gagctttctc gtaccatcga aactatcggc ccggtaaaag
     1141 gggcgcgcgt acatctggca atgccgaaac cgtctttatt cgtccgtgaa caaaaatccc
     1201 cttctgcatc ggtgacggta aatctattac ccggccgcgc actcgatgaa gggcaaatta
     1261 gcgccattgt acatctggtt tccagcgcgg ttgctggtct gccgccgggg aacgtcacgc
     1321 tggtggatca gggtggacat ctgttaaccc tgtccaatac cagcgggcgc gatcttaatg
     1381 acgctcagtt gaaatatgcc agcgatgtcg aaggccggat tcagcggcgt attgaagcga
     1441 tcctgtcgcc aattgttggt aacggtaata ttcacgccca ggtcacggcg caactggact
     1501 tcgccagtaa agaacaaacg gaagaacagt atcgccctaa cggtgatgaa tctcatgcgg
     1561 cgcttcgttc acgccagctt aatgagagcg agcaaagcgg ttccggttat ccgggcggcg
     1621 taccgggggc gttgtcgaat caaccggcac ctgcgaataa cgcgccaatc agcacgcctc
     1681 cggcaaatca aaataaccgc cagcagcagg cgagcaccac cagcaatagc gggccgcgta
     1741 gcacacagcg gaatgaaacc agtaactacg aagtcgatcg caccattcgt cataccaaaa
     1801 tgaacgtggg cgatgtgcaa cgtctgtcag tcgcggtagt ggtaaattac aaaaccttgc
     1861 cagacggtaa accattgcct ctcagcaacg aacagatgaa gcaaattgaa gatctgaccc
     1921 gcgaggcgat gggcttttct gaaaaacgcg gtgactcgct caatgtcgtt aactcgccgt
     1981 tcaatagcag tgacgaaagc ggcggagaac tgccgttctg gcaacagcaa gcgtttatcg
     2041 atcagttgct ggctgccggt cgctggttgc tggtactgct ggtggcgtgg ctgctgtggc
     2101 ggaaagcggt acgcccgcag ttaacaagtc gcgccgaggc gatgaaagct gtacagcaac
     2161 aggcgcaggc ccgtgaggaa gtggaagatg cggtggaagt ccgcctgagc aaagacgaac
     2221 aactccaaca acgacgcgct aaccaacgtc tgggggcaga agtcatgagc cagcgtatcc
     2281 gtgaaatgtc tgataacgat ccgcgcgtgg tggcgctggt cattcgccag tggataaata
     2341 acgatcatga gtaacctgac aggcaccgat aaaagcgtca tcctgctgat gaccattggc
     2401 gaagaccggg cggcagaggt gttcaagcac ctctcccagc gcgaagtgca aaccctgagc
     2461 gctgcaatgg cgaacgtcac gcagatctcc aacaagcagc taaccgatgt gctggcggag
     2521 tttgagcaag aagctgaaca gtttgccgca ctgaatatca acgccaacga ttatctgcgt
     2581 tcggtattgg tcaaagctct gggtgaggaa cgtgccgcca gcctgctgga agatattctc
     2641 gaaactcgcg ataccgccag cggtattgaa acgctcaact ttatggagcc gcagagtgcc
     2701 gccgatctga ttcgcgatga gcatccgcaa attatcgcca ccattctggt gcatctgaag
     2761 cgcgcccagg ccgccgatat tctggcgttg ttcgatgaac gtctgcgcca cgacgtgatg
     2821 ttgcgtatcg ccacctttgg cggcgtgcag ccagccgcgc tggcggaact gaccgaagta
     2881 ctgaatggct tgctcgacgg tcagaatctc aagcgcagca aaatgggcgg cgtgagaacg
     2941 gcagccgaaa ttatcaacct gatgaaaact cagcaggaag aagccgttat taccgccgtg
     3001 cgcgaattcg acggcgagct ggcgcaaaaa attatcgacg agatgttcct gttcgaaaat
     3061 ctggtggatg tcgacgatcg cagcattcag cgtctgttgc aggaagtgga ttccgaatcg
     3121 ctgttgatcg cgctgaaagg ggccgagcag ccactgcgcg agaaattcct gcgcaatatg
     3181 tcgcagcgtg ccgccgatat tctgcgcgac gatctcgcca accgtggtcc ggtgcgtctg
     3241 tcgcaggtgg aaaacgaaca gaaagcgatt ctgctgattg tgcgccgcct tgccgaaact
     3301 ggcgagatgg taattggcag cggcgaggat acctatgtct gataatctgc cgtggaaaac
     3361 ctggacgccg gacgatctcg cgccaccaca ggcagagttt gtgcccatgg tcgagccgga
     3421 agaaaccatc attgaagagg ccgaacccag ccttgagcag caactggcgc aactgcaaat
     3481 gcaggcccat gagcaaggtt atcaggcggg gattgccgaa ggtcgccagc aaggtcatga
     3541 gcagggctat caggaaggac tggcccaggg gctggagcaa ggtctggcaa aggcgaagtc
     3601 tcaacaagcg ccaattcatg cccggatgca gcaactggtc agcgaatttc aaactaccct
     3661 tgatgcgctt gatagtg
//
LOCUS       DADLFA010000256         3661 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000256 DADLFA010000000
VERSION     DADLFA010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.258"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            42..1238
                     /gene="hemY"
                     /locus_tag="JQH65_003871"
     CDS             42..1238
                     /gene="hemY"
                     /locus_tag="JQH65_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HAY6141120.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(2162..3397)
                     /locus_tag="JQH65_003872"
     CDS             complement(2162..3397)
                     /locus_tag="JQH65_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HAY6141121.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAASDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(3544..3620)
                     /locus_tag="JQH65_003873"
     tRNA            complement(3544..3620)
                     /locus_tag="JQH65_003873"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3584..3586),aa:Pro,seq:tgg)
BASE COUNT          971 a          939 c          891 g          860 t
ORIGIN      
        1 caaggtgata ctccggcagc cgcgccgcaa ggagaataac catgctaaaa gtgttattac
       61 tctttgtttt gctgattgcg gggatagtgg ttggcccgat gattgccggt catcagggtt
      121 atgtgctgat ccagaccgac aactacaata tcgaaaccag cgtcacgggc ctggcgatca
      181 tattgattct ggcgatggta gtgctgtttg ccattgagtg gctactgcgg cggatcttcc
      241 gcactggcgc gcacacccgt gggtggtttg tcgggcgtaa gcgtcgccgt gcacgtaagc
      301 agaccgaaca ggcgctgctg aaactggcgg aaggcgatta tcagcaagtt gaaaagctga
      361 tggcgaaaaa tgccgatcac gctgaacaac cggtggtgaa ctatctactg gctgccgaag
      421 ccgcgcaaca acgtggtgat gaagcgcgcg ccaaccaaca tctggaacgc gcagcggagc
      481 tggccggcaa tgacaccatt ccggtagaaa tcacccgtgt acgtctgcaa ctggcccgta
      541 atgaaaacca tgctgcacgc cacggcgtgg ataagctgct ggaagttacg ccacgccatc
      601 cggaagtatt acgtctggcg gaacaggcgt atatccgcac aggtgcatgg agttctctgc
      661 tggatatcat cccatcaatg gcgaaagccc atgttggtga tgaagaacat cgtgcaatgc
      721 tggaacaaca ggcatggatt ggcctgatgg atcaggcgcg tgccgataac ggtagtgaag
      781 gtttgcgtaa ctggtggaaa aaccaaagcc ggaaaacgcg tcatcaagta gcgttgcagg
      841 tggcaatggc ggaacatctt attgaatgtg acgatcatga tactgcccag caaattatca
      901 tcgatggcct gaaacgccag tacgacgatc gcctactgct gccgattcct cgactgaaaa
      961 ccaacaatcc ggaacagctg gaaaaagtgc tgcgccagca gatcaaaaac gtcggcgatc
     1021 gcccgctgtt gtggagcaca ctgggccagt cactgatgaa gcacggagaa tggcaggaag
     1081 catcgctcgc cttccgcgca gcgctgaaac aacgtccgga cgcctacgat tacgcatggc
     1141 ttgccgacgc gctggacaga ctgcacaagc cggaagaagc tgcagcgatg cgtcgcgacg
     1201 gtttgatgtt aacgttgcag aacaacccac cacagtagtt ccttctcacc cggaggcaag
     1261 cacctccggg acctttctga tgcataaaaa aacgcctgct cttattacgg agcaggcgtt
     1321 aaaacaggtc tgtatgacaa caagtgggtg cttcactcaa cgttgtgtcc atggtgtctg
     1381 atgaggcata agcgacatct gtcagtggac gataagcacc gtaaacggct ctgcgtcatt
     1441 ccggagttta tgaggcacta aggcgaacat aagagatgga atgagcatct actcgtttat
     1501 tatgccacag agaatcggga aataacatcc cttaacactt gttatgagat aattctgtaa
     1561 tcctctttgc ttcctgagta ataacttcct gagtgaatat ttaacctgag cttgatccta
     1621 cacatactta ttatgaatga taaaattcat tcaattaata acacatatat taattgccgt
     1681 taaaactaaa aaaagcatca ataatcaacg cgatataata aacctgcctt acatatcaac
     1741 tgcgccagag gtaggattga aaacgctctc ctgattttcc aattcatttt ctggataaat
     1801 aaataattta tttttgtcac tattatttat gtaatcatcc tgtcagggag agggatctca
     1861 attatcaatg cttaattacg tcatcatttt gatgacatga aaccggagca ttttgcatgg
     1921 aattttcgtt ttcacccaaa cttcttgttg ttgctgtcgc cgccgctctt cctctcatgg
     1981 ccagcgcagc agataccccg tcaactgcca ccgcacgcaa aggctttgcc ggatacgatc
     2041 acccaaacca gtatccacag aacgctgcaa tttattgaat ttgcacgatc atgtaggccg
     2101 gataaggcgt ttacgccgca tccggcaatc aaccgcaggc ggccacaaaa tggccgctat
     2161 tttacttatt caccaccagc aaatgtgcct gcataatgtc actggccggg cgaccgtgcg
     2221 ccagcaaatc agccattgct ttaagatatg gcggtagatg gcggaaataa cgctgatacc
     2281 cggcacacaa ataattcagc cccggtttgc cgctggcatc gagcatgaag cggtgtttcg
     2341 ggcagcctcc ccagcacgct tttaacacgt tacaactgcg acactgcgcc ggtaattgct
     2401 taaatttatc ttcaccaaac acctgctgtt gcggggaatc gatcatttct gcaattgttt
     2461 gctgatgcat attccccagc cgatattgcg gataaacata gtgatcgcag gcgtaaacgt
     2521 cgccgttgtg ctcaacaatc accgagcgcc cacaggttgg ctgatgatgg caaaccgcac
     2581 ccggcgcacc gacaaaattg gcaaacgccc attcgatatt catcacgaaa atcttgccga
     2641 cgtcgcgttt gatccagtgg tcaaaaatcg ccaccagaaa ttcaccaaat tcatcggggc
     2701 gcacagacca ttccgtcagt tccccctgaa tatcaccagg cgcatgtagt ttcagtccat
     2761 cgctggcagc tgtttcatca gccaggcgct cgaccaccgg aataaactgg atgaattcga
     2821 ctcccgcatc gcacaaaaaa tcataaacct gcaacggttg ctgcgcgctg gtgcggttga
     2881 cgcagaccag cacgttatag tcgacatgat gtttttgcag cagcgtcagg gcacgcatca
     2941 ccagcttatg cgttggtctg ccacctttgg tcacgcgata ttgattgtgg atctcagccg
     3001 gaccgtccag cgataaccca acaagaaaat gattttctgc cagaaatgca caccatttat
     3061 catcgagcag cacgccgtta gtctggaagc tgttacttat cttcctgcca gcaccgtatt
     3121 tcgcctgtaa ctttacggca cggcggtaaa aatccagccc gagtaacgtt ggttcgccgc
     3181 cctgccaggt aaaagccact tcgttttgcg gttcgctggc agcgatatag tgacggacat
     3241 acgcttccag cgtgtcatcg tccatatgcg tgactggctt ttcgcggtaa agggattgtt
     3301 tttcgagata aaaacagtag tcacagttca gattacaatc ggaaccactc ggtttcgcca
     3361 tcacatgaaa agcacgcgtt ggaacctgtt gcagcatggt cactccttgc ctgaagtcat
     3421 ccttcaatac gttaagggcg aaatcatacc gtggggcaat gagttattac gggcgtttat
     3481 tgcgaagaga gggtgacgaa atgcacagaa aacaaaaaac cccgccgaag cggggttcaa
     3541 aattggtcgg cgagagagga ttcgaacctc cgacccactg gtcccaaacc agttgcgcta
     3601 ccaagctgcg ctactcgccg aaatactgct ttttgaattt ttagttcaat tatttaaagt
     3661 c
//
LOCUS       DADLFA010000257         3589 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000257 DADLFA010000000
VERSION     DADLFA010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.259"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(625..2334)
                     /locus_tag="JQH65_003874"
     CDS             complement(625..2334)
                     /locus_tag="JQH65_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HAY6141122.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVKVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGCSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2766..3485)
                     /locus_tag="JQH65_003875"
     CDS             complement(2766..3485)
                     /locus_tag="JQH65_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HAY6141123.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQFFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
BASE COUNT         1067 a          720 c          639 g         1163 t
ORIGIN      
        1 ataagcttga tacaactggt ctggtatata cagtttcttt gagtgcggtt acgtccacag
       61 aggtaacggt aaaagtaacc ccttgtcggg accactccga ccgcgcacag aggcattttc
      121 atcgctgaca ggccgcattg cctcgcaggg ctggaggagg atcggtgata tccggacgac
      181 cgtaccggct tacgccgggc ttcggcggtt ttactcctct gccatgtgca acaacggagt
      241 gccgccttac atgccgctgg cgcggcatat cctggtaaca tttttgttga taccaccttc
      301 cggccttatc ctgaccagtg ggctttcctg gcatcgatga taccgatgaa cgcgcatgat
      361 attgagccga cgatattacg ggcaactgga aatactcacc ctctggatgt gacctttatc
      421 catgaagaag atttggccat accatggaaa cctgaacaat catcagttta cgcccatgta
      481 aacccctacg ggatatttga actcgatatg gaaacaaggt tgccaatcga ggtggttgcc
      541 tgaaagttgg cagttacccg gtcactacac cttgttggag tgaacatatc aaaaacaggt
      601 agttacacaa tttaaattac agtctcataa aaaatgatta aatctattta ttatttcaat
      661 aaacgttttt ttcatcttct ttgaaaattg ttctgctata gtatccggtt ccagattgtt
      721 ctcttttatt attctacaat actctaatat tgtttctttg tctctattta ctacagatac
      781 aaatacacca ggaataccat ctttattagt tgcggacagc atttcgacca atcgtcttgt
      841 ggtaaaacta tagtttttta gaatattaca aaaggcacgt attgtttcgg tatggccatt
      901 ataaaatgcc ataaaaagtc ctgatgtacc gttgggattt tttatccata ataattcatc
      961 tatatattcc tggtgagggg ggagtatttt tagtatatct ccatacgcca taatggcctt
     1021 tgtgtgccca ttttgaagtg ccataaacaa cccatgcttt actttcaata attctttaat
     1081 ttgttcacta ctaagcattt tcttattaag cagacttccg aacatacgaa tactatctgc
     1141 atgtccattc tgcaatgcaa aaaataaccc aggtaaatca tggctgtctt tcgcagctaa
     1201 tagatataat ctcttatcgg aagttaacgg ggttttaaag actatttccc cataggcttt
     1261 tattgtgtct acatgtccgt tcataaaaga catgcataaa cccggcacat tgtttgcccc
     1321 taccgcagca agcaatgtaa gtattgtttc ttgactaaga ttgcacatat ttaatatctt
     1381 accatatact tgaataatgt ctgaacatcc gttttgaagt gctatataaa atgcaggagt
     1441 accatcagag cttctcgtgg ccagaaggtt caccagttta gattgagaaa tttcaccatt
     1501 tttgtatttt tccatcagta taaataatac ttctgtcaga ttctttgtta tcgcatgata
     1561 tataactctg gaattgatca attcatctgg taaaagtttc actgtgtttg ttagagggga
     1621 attatgccta tcttcaaata ttacctgccc gggttgctcc agaccataaa ttttccaata
     1681 aaaatcatca aaaaaatcct tagcggataa ttgactcagt tcgaaatggc tatcacaagt
     1741 aaatacagta cgtttatgag taacagttcg attaggatcg aaaaactgaa caacccattt
     1801 ttcactttct tgtacgcgct ttattttcaa ccctaacgcc atttggtggt ttaatgtgca
     1861 tgctgataat gttctgacaa agtctccatt ttttttcatg ttataaaaaa aattagtaat
     1921 aactgttccc cactttttat tttccacaaa ataaatataa gctggtgatt ttgaaaaatc
     1981 ttccgttttt tccaaacata cattttttgc aatagcatca ggggtgctaa gaagctgata
     2041 gtttaccttc ccatttgttt ccataaaatt catttcccaa taggaagcta ggtgacggca
     2101 aactatttgt cttccatata tatcttttgc cactccattt atatttacct tcacatttct
     2161 cttttctgaa aaataaggtg ctgttttatt cttaaactga attagttcac tggagtttga
     2221 gaccctcata atagaacttg ttattgttct attatctctg ttaacagaca tatctgttga
     2281 taacgaattt tttgttgaaa atatactaga tgaaaatgtt ttatttaacg gcatagaaga
     2341 caaccataga gatgttataa tactattaga ttgcagttac taatagtaaa agcctcctta
     2401 caaggaggta gcttattcag aatgctccta taagatagcg agctcgtcaa cgatacagat
     2461 gatctgatta tattgtgcaa aaggagaaca ggaaaatagg cactggaaaa gatgaaggcc
     2521 tgatgggagc attaactaat gcaaaatccg acaggttaat atcccgaaaa gcaatcaaac
     2581 gactcaaaca ggagatgcac gctgaagtgg tggacaactg ttcggatttc agatgagtag
     2641 ctcagcagtt tatgagccga atgctctgtc acggtcagga atcaggctgc atacgctgtg
     2701 ccaaatctaa tcgtagttat tctaaccagt tttccagcta caaggtggtg tagctggcat
     2761 cttctctact ctatcatcaa acgataaaat ggttcctctc gtaacatttg ctcttgcctt
     2821 tcacttccat ctcgatcgct tcgtagttca ttacgatagc tgacatattt ccagtcttca
     2881 ggtctaacat ctgacgccgg atactccccg ggagcgattt tgcttcttaa tagattagac
     2941 tcgagacaca ttataaattg tcgtatttta tgaagtaata aagcactata ttgcgagcat
     3001 tcctgatctg attttacata tagcgtaaac tgagctccta tccccacacg agattgctga
     3061 gaaactcgat tcatatcagt tatcttccat ttatctatag gactgtcctc tgaaaataat
     3121 aaaccagaca aaatttggaa tgccagagga acttgctccc tagctatact aatatgaaac
     3181 ttgtccccaa caaaatcccc cttactctga ggattttcac gacatgcatg aataaacaca
     3241 tctccactat ggttcacaat ctggaatcct tggtacatga cagaaaaaaa ttgacatata
     3301 tcagagtaag ttggcacgtt cgatgctact ttgaaatagt acgctgggta ttgccgtatt
     3361 tggttgtaca atatattgaa gttatttttc aatctttcat tcgcagaaag catctgcggt
     3421 atttcagacc tcacaacatt caaagaatct aaatttagtt ttagacaggg cttttttatg
     3481 ggcatagaaa acgtcctcta taaaatagat atattatctt atattgattg tttatcatct
     3541 tttataaaac gatacattat atgtaatgta tgtaagcgta agggtcttc
//
LOCUS       DADLFA010000258         3540 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000258 DADLFA010000000
VERSION     DADLFA010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.260"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            232..543
                     /locus_tag="JQH65_003876"
     CDS             232..543
                     /locus_tag="JQH65_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAY6141124.1"
                     /translation="MFNVITHPAALDELLELPDNLRGRMTRLIERLEQEGNKMKMPHS
                     RVIGGGLFELRVGDKNIARTLYAYATGNEIYLLHAFVKKTQKTPTKAIEIARMRLKEM
                     N"
     gene            543..830
                     /locus_tag="JQH65_003877"
     CDS             543..830
                     /locus_tag="JQH65_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY6141125.1"
                     /translation="MKVKGVPFSQVKQKLLSTPEAVKGYEDADKELEMVEMLYEMREK
                     AGLTKSALAERMGLRPSAISRLESNPLGASMKTLTKYAHACGATIDIHAVY"
     gene            complement(1367..2554)
                     /locus_tag="JQH65_003878"
     CDS             complement(1367..2554)
                     /locus_tag="JQH65_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAY6141126.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPRIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKR
                     EWNRFLDTHYRRGWNVNVSRVMDNATHVAVYFGSYLKKPPVPVSRLEHYAGQDEIGLR
                     YNSHRTKREEYLLMSGDEFMERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIITE
                     TVRKTAMQITWRGMYQRLLKVDPLKCVLCGSQMRFTGLKRGYRLAEQVLMHEPLARMR
                     WCG"
     gene            complement(2554..2919)
                     /locus_tag="JQH65_003879"
     CDS             complement(2554..2919)
                     /locus_tag="JQH65_003879"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141127.1"
                     /translation="MARSAKPRKRKPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFDS
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILSGISAGG
                     MYCPECGTVHWPDGVAPPF"
     gene            2938..3333
                     /locus_tag="JQH65_003880"
     CDS             2938..3333
                     /locus_tag="JQH65_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY6141128.1"
                     /translation="MTLPVFITVIADHDKPQPSGCLLELQLNKGEYRHKLPRWIFFAN
                     QGEFTTGDYEEIMNKASALSLVSNAILYWNTSRISNIVEGLRENGEVIDDDVLAHISL
                     LPYKHVLPNGTYFVDGEPYGTEPTERAKP"
     gene            3363..3446
                     /locus_tag="JQH65_003881"
                     /pseudo
     CDS             3363..3446
                     /locus_tag="JQH65_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
     gene            3446..>3540
                     /locus_tag="JQH65_003882"
     CDS             3446..>3540
                     /locus_tag="JQH65_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAY6141129.1"
                     /translation="MEQKWTSKALSDLARLYDFLVLASKPAAARMV"
BASE COUNT          926 a          919 c          835 g          860 t
ORIGIN      
        1 aaggtgaaat ctgtcaatca gtaccggctt acgccgggct tcggcggttt tactcctgtg
       61 ccatatgcaa caacggagtg ccgccttcca tgccgctaac gcggcatcaa aaaatccctc
      121 ttctccagtt gcccccctcc gaactcagag tgtattttca tttattgatg aaatttactg
      181 tttgcgcaat tttcctgttt tagtgtattt tgtcattctg gaggctataa tatgtttaat
      241 gtgattactc acccggccgc actggatgaa cttctagaat taccagacaa tctgcgtggt
      301 cgtatgacca gactgattga acggctggaa caggaaggta acaaaatgaa aatgcctcac
      361 agccgtgtaa ttggcggagg gctttttgaa ctgagagtcg gggataaaaa tatcgcaagg
      421 acactgtacg cttatgcaac gggtaacgaa atctatctgc tgcatgcatt tgtgaaaaaa
      481 acgcaaaaga caccaacgaa agctattgaa attgccagga tgcgtctgaa ggagatgaac
      541 tgatgaaagt aaaaggtgtt ccattcagcc aggtaaagca aaaactactc agtactccag
      601 aagctgtcaa agggtatgag gatgctgaca aagaactgga gatggtggaa atgttatacg
      661 aaatgagaga aaaagcggga ttaacaaagt cagcactggc cgaacgaatg gggttacgcc
      721 cgtcagccat cagtcgcctg gaaagtaacc ctttaggggc cagcatgaaa actttgacaa
      781 aatatgctca tgcctgtggc gcaaccatcg atattcacgc tgtctattga ggggtccgct
      841 tggaattcgg aaaataaacc acgttaagac tattttttag ataaatgact ttcaggaatc
      901 gccaagatgg cgggataaca gcggttttgg tgtcctaaat cgtacaattc tgtacatcca
      961 cgcaagggaa tgtacggaag taagccagac ggttatcgga cggtcagaac atttcgtaca
     1021 gcctttacgt tcagtgagtt agctggcctg gacgtaacca ctcgttagct caattctctt
     1081 agcgtgggaa aatttccgaa ttccaagcgg acccctttat ccttgccccg ctgaatgagg
     1141 aaacgctgtt ccgggggatt atgctgaacg tcttccgttc atcgatagga attaaaaccc
     1201 caaaaagatt aaaaaacacc acaaaacgga tgtttcttca acaccacttt tgctcatatg
     1261 aacggaaccg acgattaaac tggatggctc tgattgattc agggtatgaa tggcggtttt
     1321 ttgctccgtt tccctcaaaa tggacgctac ttaccctctg cggctctcag ccgcaccacc
     1381 gcatccgggc cagcggctca tgcatcagga cctgctctgc cagacggtag ccccgcttca
     1441 gccccgtaaa acgcatctga ctcccgcaca gcacgcactt cagcgggtca accttcagta
     1501 acctctgata catccctctc caggtgattt gcatcgccgt ttttctcact gtctccgtta
     1561 tgatgtacac cacttcttcc agtaaccgcc gtttcgccgg actcagaaaa ccgtagtacc
     1621 tcaccatacg gaacccctta tccgccacat gccaggagaa cctttccatg aactcatctc
     1681 cactcatcaa caggtattct tcccgttttg ttcggtgact gttgtaacgc agtccgattt
     1741 catcctgacc ggcataatgc tccagacgac tcaccggcac cggtggcttt ttcaggtaag
     1801 agccaaagta caccgccaca tgggtggcat tatccatcac ccgggatacg ttgacattcc
     1861 agccacggcg gtaatgcgtg tccaggaagc gattccattc ccgtttactg cttccttctg
     1921 ctgccagcgc atccggcatc accaggtcag ggtatttccg tgacagtaac cgtgttatcc
     1981 ggtagcgcca catgctcatc accttacggg cgtaaaaatg aagatttttc caggtgtggc
     2041 ccgacgtcac accaccggca gttgtcgata aatggatatg cggatgccac tgctggtcac
     2101 gcccccatgt gtggatcacc gtgaatatcc tcggctccac atctgcctga tggcagattt
     2161 ccagtatcac atccgctgca atgcggctca tctctgccag caaccaccgg ttgtggaaca
     2221 ccagggacca gtactggcag ggtaacgtga acacaatatg ctgccatggg cagtcgggga
     2281 ccaggctcag cagatactgt atccactgtg cgccagcctt caccccgcag tgcgggcagg
     2341 agcggctttt acaccggaag cagacctttt ttgtatggca acagtccggt gatgaacagc
     2401 accactgtgt ataccccatc agtgtggtcc cgcacgccat gattttggtc accgactcaa
     2461 tcaccaccgg acgtactgcc ccttccggct gcttctccag ccagttaagc cagcggtttc
     2521 cctgctgaaa gatatcggca aaacggggaa gcatcagaag ggcggggcga ctccgtccgg
     2581 ccagtgaacc gtgccacact ccgggcagta cataccgccg gcgctgatac cggaaagaat
     2641 ggtcgcaaat tcccgctccg tgcagcgggc aatttccgga tacccttcgt catcaacacg
     2701 tacaaaccag aagaccagtt ttttgtttcc ggcatccaca aagaacggaa tattcaggtc
     2761 tgcgcaacac tcaacggcat catcaaaact atcaaagcgc agaacttctg cgtcttcttc
     2821 gtcaaaaaaa tcatcttcgt gaagcttcac gacatagcgg ggaagtttgc ttctttgaga
     2881 ggcgggttta cgtttacggg gtttagctga acgggccata taaccacctg aaagacaatg
     2941 acattgcctg tttttataac ggtaattgca gaccatgaca agccgcagcc gtcaggctgc
     3001 ctactcgagc ttcagctcaa taaaggcgaa tatcgacata aattaccccg atggatcttt
     3061 tttgcaaatc agggtgaatt cacgaccggt gattacgaag aaatcatgaa taaggccagc
     3121 gccctgagtc tggtatcgaa tgcaatcctg tactggaata catcaagaat aagcaatatt
     3181 gttgaagggt tacgtgaaaa tggtgaggtt attgatgatg acgtattagc ccatatatca
     3241 ttactgccct ataaacacgt attacctaat ggtacttatt ttgttgatgg agaaccttac
     3301 ggtactgagc ctaccgaaag agcaaaaccg taagtttctg ttcaaatgct cccgctggtc
     3361 catccggaca gattatcgac catcaggctg tacaggcctg ggcggacagc ctcagtactg
     3421 acaatccgtt accggtgcca cgctgatgga acagaagtgg accagtaagg cgctttctga
     3481 tttggcgcgg ttatatgatt ttctggtgct ggccagtaaa cctgctgccg ccagaatggt
//
LOCUS       DADLFA010000259         3484 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000259 DADLFA010000000
VERSION     DADLFA010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.261"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            1..1947
                     /locus_tag="JQH65_003883"
                     /pseudo
     CDS             1..1947
                     /locus_tag="JQH65_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
     gene            complement(1990..2463)
                     /gene="ivy"
                     /locus_tag="JQH65_003884"
     CDS             complement(1990..2463)
                     /gene="ivy"
                     /locus_tag="JQH65_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-lysozyme inhibitor"
                     /protein_id="HAY6141130.1"
                     /translation="MGRISSGGMMFKAITTVAALVIATSAMAQDDLTISSLAKGETTK
                     AAFNQMVQGHKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEK
                     SNQMTGLFSTIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            2617..3387
                     /gene="yafV"
                     /locus_tag="JQH65_003885"
     CDS             2617..3387
                     /gene="yafV"
                     /locus_tag="JQH65_003885"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HAY6141131.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFRLR"
BASE COUNT          778 a          891 c         1000 g          815 t
ORIGIN      
        1 atgattatca aaaaagagta cggctgactg gagttctcag cttatgccca gtctcgcgtg
       61 ctgcaaaaac tctccggcgt gagcgggatc ctggcgatta ccgtcggcgt gccgaactca
      121 ttaggcccgg gcgagctgct gcaacattac ggcactgacg agcagaaaga tcactatctg
      181 ccgcgtctgg cgcgtggtca ggagatccct tgctttgcac tgacaagccc ggaagcgggt
      241 tccgatgcgg gcgcgattcc ggacaccggg attgtctgca tgggcgaatg gcagggccag
      301 caggtgctgg ggatgcgtct gacttggaac aaacgctata ttacgctggc accgattgcg
      361 actgtgcttg ggctggcgtt taaactctcc gccccggaaa agctgctggg tggcgcagaa
      421 gatttaggta ttacctgtgc gctgatcccg accaccacgc cgggcgtgga aattggtcgt
      481 cgccacttcc cgctgaacgt accgttccag aacggaccga cgcgcggtaa agatgtcttc
      541 gtaccgatcg attacatcat cggtgggccg aaaatggccg ggcaaggctg gcggatgctg
      601 gtggagtgcc tctcggtagg ccgcggcatc accctgcctt ccaactcaac cggcggcgtg
      661 aaatcggtag cgctggcaac aggcgcgtat gctcacattc gccgtcagtt caaaatctct
      721 atcggaaaga tggaagggat tgaagagccg ctggcgcgta ttgccggtaa tgcctacgtg
      781 atggatgctg cggcatcgct gattacctac ggcattatgc tcggcgaaaa acctgccgtg
      841 ctgttggcta tcgttaagta tcactgtacc caccgcgggc agcagtcgat tattgatgcg
      901 atggatatca ctggtggcaa aggcattatg ctcgggcaaa gcaacttcct ggcgcgtgct
      961 taccagggcg caccgattgc catcaccgtt gaaggggcta acattctgac ccgcagcatg
     1021 atgatcttcg gacaaggagc gattcgttgc catccgtacg tgctggaaga gatggaagcg
     1081 gcgaagaaca atgacgtcaa cgcgttcgat aaactgttgt tcaaacatat cggtcacgtc
     1141 ggtagcaaca aagttcgcag cttctggctg ggcctgacgc gcggtttaac cagcagcacg
     1201 ccaaccggcg atgccactaa acgctactat cagcacctga accgcctgag cgccaacctc
     1261 gccctgcttt ctgatgtctc gatggcagtg ctgggcggca gcctgaaacg tcgcgagcgc
     1321 atctcggccc gtctggggga tattttaagc cagctctacc tcgcctctgc cgtgctgaag
     1381 cgttatgacg acgaaggccg taatgaagcc gacctgccgc tggtgcactg gggcgtacaa
     1441 gatgcgctgt atcaggctga acaggcgatg gatgatttac tgcaaaactt cccgaaccgc
     1501 gtggttgccg ggctgctgaa tgtggtgatc ttcccgaccg gacgtcatta tctggcacct
     1561 tctgacaagc tggatcataa agtggcgaag attttacaag tgccgaacgc cacccgttcc
     1621 cgcattggtc gcggtcagta cctgacgccg agcgagcata atccggttgg cttgctggaa
     1681 gaggcgctgg tggatgtgat tgccgccgac ccaattcatc agcggatctg taaagagctg
     1741 ggtaaaaacc tgccgtttac ccgtctggat gaactggcgc acaacgcgct ggcgaagggg
     1801 ctgattgata aagatgaagc cgctattctg gtgaaagctg aagaaagccg tctgcgcagt
     1861 attaacgttg atgactttga tccggaagag ctggcgacga agccggtaaa gttgccggag
     1921 aaagtgcgga aagttgaagc cgcgtaattg aataacggag ccgaaaggct ccgtttcttt
     1981 atccgctaat tatttaaaat taaagccatc cggatggttt tccaggctgc cggtcaacgc
     2041 cgcgaacagc accgttttac catcaatcga aagcgcatcg ttcacattca gccaggtgag
     2101 tttctcttgc gacgttttct catcaatagt cgagaacagc cccgtcatct gattagattt
     2161 ctcggaccac atcacagcga tacgttgcga gccacagtca tgcggtttgc acgcgctcat
     2221 cacctgatac gtctcatctc ccaacgttac ggtttgtgcg ggagtataag taccgccttt
     2281 catcacccag gcaggcagct tatgcccttg taccatctga ttaaatgcag ctttggtggt
     2341 ttcgcccttt gcaaggctgc taatggttaa atcatcctgc gccattgcac tggtggcgat
     2401 gaccagagcg gcgactgtcg ttattgcctt aaacatcatt cctcccgagc ttatcctgcc
     2461 catgttatta accttctaaa gcctggcgat aacacgataa tttgcaaatc agatatttcc
     2521 aatatcagca tttgttatca atttattctg cgctaaggca actgcctgat aaggtgttaa
     2581 agttataaaa cccttcgcat aaaggagttg tacaccgtgc ctggtttgaa gattacgctt
     2641 ttgcagcaac cactggtgtg gatggatggc cctgccaacc tgcgtcattt tgatcgtcaa
     2701 ctggaaggta ttaccgggcg cgatgtgatc gttctaccgg agatgtttac cagcggcttt
     2761 gccatggaag cggcagcttc gtcgctagca caagatgacg tagtgaactg gatgacagct
     2821 aaggcgcagc agtgcaatgc gctgatagca ggcagtgttg cattacaaac ggagtctggt
     2881 tcggttaacc gctttttgct ggttgagccg ggcggcacgg tacattttta tgataagcgt
     2941 catctgttcc gcatggcaga tgagcatcta cattataaag cgggcaatgc gcgagtgatt
     3001 gtggaatggc gcggctggcg tattttgccg ttggtgtgct acgacttacg ttttccggtg
     3061 tggtcgcgca atctcaacga ttatgacctc gccatctacg ttgccaactg gcctgctccg
     3121 cgctctctgc actggcaggc attgctgacg gcccgcgcga ttgagaatca ggcgtatgtg
     3181 gcgggatgca atcgcgtcgg cagcgatggc aacggctgcc attatcgcgg tgacagccgg
     3241 gtgattaatc cacaaggcga gattatcgct actgccgacg cgcatcaggc aacgcgcatt
     3301 gatgcggagc tgtcgatgat ggcattgcgg gaatatagag aaaagtttcc ggcatggcag
     3361 gatgcggatg agtttaggtt gcggtgagtg ggggttgtgt ttaaggctgg gggagagtcg
     3421 gggtaagact acgaaagccc gctctccgca aggactgacg ccagatagtt tctgtccata
     3481 gctg
//
LOCUS       DADLFA010000260         3348 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000260 DADLFA010000000
VERSION     DADLFA010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.262"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            392..1111
                     /locus_tag="JQH65_003886"
     CDS             392..1111
                     /locus_tag="JQH65_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HAY6141132.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVGHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            1122..2549
                     /locus_tag="JQH65_003887"
     CDS             1122..2549
                     /locus_tag="JQH65_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HAY6141133.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKNKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            2542..3237
                     /locus_tag="JQH65_003888"
     CDS             2542..3237
                     /locus_tag="JQH65_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HAY6141134.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
BASE COUNT          878 a          700 c          935 g          835 t
ORIGIN      
        1 atacaaacta aattgcttca attaaataaa atcccgacta ttacttgata cgtgattatt
       61 gtcggtgatt ttgtttgtac accatcagtg tatatctcaa taacccctga ataagtagct
      121 ctgaataggt ataagggatg tggccatttt taaatggatt tcttatgcca aaaatgatcg
      181 tcgatttctc cataaaatgt gagcgatgcc gaaagaaata aaattagtta ttgcatttga
      241 cgtttgggtg aaagattatc atttgtcata caaatgaggg gtagtatgtt gctagccaat
      301 taaaaaagaa cgccatattt attgatgatt gatcccccgg tgggggggaa acatcgggca
      361 taaatgggca tgaagtaatg gagtattagt tgtgaatgtc aatttctttg tcacctgtat
      421 tggtgacgcc ctgaaatcaa gaatggcacg agactccgtg ctgctactgg aaaaactcgg
      481 ctgtcgcgta aatttcccgg agaaacaggg atgctgcggt cagcctgcga tcaatagcgg
      541 ttatatcaaa gaagcgattc cagggatgaa aaatctgatc gccgcactgg aggataacga
      601 cgatcccatt atttcaccgg ctggctcttg cacctatgcc gtaaaaagtt acccgacgta
      661 tctggcggat gaacctgaat gggcatcacg tgccgaaaag gttgccgcgc gtatgcagga
      721 tctcacctct tttattgtta ataaattagg ggtagtcgat gtaggtgcca gtttgcaagg
      781 gagagctgtg tatcacccat cttgtagcct ggcccgtaag ctgggagtga aggacgagcc
      841 acttacgctg ctgaaaaatg tgcgtggact ggagctgttg acctttgctg aacaggatac
      901 ctgctgcgga tttggcggca cgttctcggt caaaatggcc gaaatatccg gcgagatggt
      961 gaaagaaaag gttgggcacc tgatggaagt ccgccctgag tatttaattg gtgctgacgt
     1021 gagttgcctg ctgaacatca gtgggcgatt acaacgggaa gggcagaaag tcaaagtgat
     1081 gcatattgct gaagtgttga tgagccgctg aggatataaa gatgtcgatc aaaaccagta
     1141 atacagattt taagactcgc atccgtcagc aaattgaaga tccgatcatg cgcaaagcgg
     1201 tggcaaacgc gcagcagcgt attggggcaa atcggcaaaa aatggtcgat gaattggggc
     1261 actgggagga gtggcgcgat cgggccgccc agatacgtga tcatgttctg agtaatctcg
     1321 acgcttatct gtaccagctc tcagaaaaag tgacgcaaaa cggcggtcac gtctggtttg
     1381 caaaaaacaa agaagacgct acccgctaca ttttacaggt tgcccaacgc aaaaatgccc
     1441 ggaaggtggt gaaatctaaa tcgatggtga ccgaagagat tggtgtcaat catgtgttgc
     1501 aggatgctgg cattcaggtg attgaaaccg atctgggtga atatattctc cagctggatc
     1561 aagatccgcc atctcatgtt gtggtcccgg caattcataa agatcgccat cagatccgtc
     1621 gagtgctaca cgaacgtctg ggctatgagg ggccggaaac gcctgaagcg atgaccttat
     1681 tcatccggca aaaaatccgc gaagatttcc tcagtgctga aataggtatt accggctgta
     1741 atttcgcggt ggcagagaca ggttcggtat gcctggtgac caatgaaggt aatgcgcgaa
     1801 tgtgtaccac gctgcctaaa acgcatattg cagtgatggg aatggagcgt attgccccca
     1861 cgtttgccga ggtagatgta ttgatcacca tgctggcgcg cagtgccgtt ggtgcacgtt
     1921 tgacgggata caacacctgg ctgacaggac cgcgcgaagc ggggcacgtt gatggtcctg
     1981 aagagtttca tctggttatt gtcgataacg ggcgttctga ggtgctggcc tctgaatttc
     2041 gggatgtgct gcgctgtatt cgctgcgggg cttgtatgaa tacttgtccg gcatatcgcc
     2101 atattggcgg tcatggatat ggctctattt atccagggcc aattggtgcg gtgatttctc
     2161 cgctacttgg cggctataaa gattttaaag atttacccta cgcctgctct ttatgcactg
     2221 cttgtgacag cgtgtgtccg gtgcgtattc cgctgtcaaa actgattttg cgtcatcgtc
     2281 gggtgatggc tgaaaaaggg atcaccgcaa aagcagagca acgggcgata aaaatgttcg
     2341 cttatgccaa tagtcatcca ggattgtgga aagtcgggat gatggccggc gctcatgcgg
     2401 caagctggtt tatcaatggc ggcaaaacac cactcaaatt tggcgcgatt agcgactgga
     2461 tggaagcacg cgatcttcct gaagctgacg gagagagttt ccgtagttgg tttaagaaac
     2521 atcaggcgca ggagaaaaag aatggataat cgaggcgaat ttttgaataa cgttgctcag
     2581 gcactgggtc gcccgctgcg acttgaaccg caagcagaag atgcgccgct taacaactat
     2641 gctaacgagc ggcttaccca acttaaccaa cagcagcgct gtgacgcgtt tattcagttt
     2701 gccagcgatg ttatgttgac gcgctgtgag ctgaccagcg aggcgaaggc ggcagaagct
     2761 gcaatacgtc tgtgtaaaga gctgggagat cagtcggtcg tgattagcgg tgacacgagg
     2821 ctggaggaat tggggattag tgaacgtttg cagcaggaat gcaatgccgt tgtttgggat
     2881 ccggcgaaag gtgccgagaa tatctcgcag gcagagcagg ctaaagtggg tgttgtgtat
     2941 gctgaatatg gtttaaccga atcgggaggc gtggttcttt tttccgccgc cgagcgcggg
     3001 cgttcattga gcctgctccc ggaatcttct ctttttatcc tgcgtaaaag cacaatcctg
     3061 ccgcgtgtag cgcaactcgc agaaaaattg catcagaaag cgcaggccgg tgaacgaatg
     3121 ccgtcctgca ttaacatcat tagcggccct agttcaacgg cggatattga gcttatcaaa
     3181 gtcgtcgggg ttcatggccc ggtgaaagcg gtgtatctga ttattgagga ttgctgagag
     3241 agatatgaga tctgaacata aacctgaaga aggcatacga ggttgaaaat agcgttaagg
     3301 acaacctgac ttaaccccgt tccgtcatgg ttatgctgct cttctgta
//
LOCUS       DADLFA010000261         3319 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000261 DADLFA010000000
VERSION     DADLFA010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.263"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..211)
                     /locus_tag="JQH65_003889"
     CDS             complement(<1..211)
                     /locus_tag="JQH65_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141135.1"
                     /translation="MIRFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVCISLQELQLELMVEYALFF"
     gene            complement(214..981)
                     /locus_tag="JQH65_003890"
     CDS             complement(214..981)
                     /locus_tag="JQH65_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141136.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERAILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNVITTQINAWARNNHPLLKERARQAIEN
                     PLKQPEPMTEYQVWEDDWIIDECLCAMYDASSESLGK"
     gene            complement(984..1694)
                     /locus_tag="JQH65_003891"
     CDS             complement(984..1694)
                     /locus_tag="JQH65_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141137.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHSNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTHDQRARG"
     gene            complement(1710..3215)
                     /locus_tag="JQH65_003892"
                     /pseudo
     CDS             complement(1710..3215)
                     /locus_tag="JQH65_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472437.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
BASE COUNT          934 a          797 c          728 g          860 t
ORIGIN      
        1 tgaaaaaaag tgcatattca accattaact caagttgtaa ttcctgaaga gaaatacaca
       61 caaccaggag cggaaaggag aatattattt ctgccagaaa agttaacgtg aatataagta
      121 cggctaagac aataaccgtc ggccatacca ataatggcac gataacaaaa ccgagaatta
      181 cagggggaat ataaagccgg aatctaatca tttttatttt cctagtgatt cggatgatgc
      241 gtcatacatc gcgcataaac actcatcaat tatccagtca tcttcccaga cctgatattc
      301 tgtcattggt tcgggctgtt ttagcgggtt ctcaatcgct tgccgcgctc tttctttcag
      361 taaaggatga ttatttctag cccaggcatt gatttgggtg gtaatgacgt tatcgatatc
      421 gtcctggtta tctctgatat aatcctcaca atatctgttc aataaacagg caagagaaac
      481 ggtcgaccaa aagccgggaa ctgaattaat ggcatccagc aatggcgcaa gagcgatatc
      541 ggcacggaaa aacatttcca gcatatactg taattcgaca gcttcatcgc cggataccca
      601 atcagtcata tactggcagg caaatcgctg taaaattgcc cgttcatccg cacgccagtt
      661 ttctttgttg gcaaggttga tacggcgcag agaaaaaact acgtgaatat tgtcaaaatg
      721 cccctgtgca acaaattcca gtaaacgcgg cagaaaatag cggacctcat cgctattttg
      781 cggatcaggc cctgggctac tattccaggc attgattaag gaatacggga tcgctcgtaa
      841 cgatgtactc cgtaatgcct tttgttcctg ctgactcagg caatattcac aacaaaccac
      901 aaatttctgc ggcacccgat aacgacgaaa ggtgtgataa atctgctcaa ttaatgcctg
      961 catggcgggg ctgggttgca tagttacccc ctcgcccgct gatcgtgtgt aaacggttga
     1021 tagacaaaac gagcattcgc cgcgttcagg cgaataaggg cgctacgcga aagggaaaag
     1081 ctggggcgaa tgcaactatt gcagtctcga cagctttcct ctgcgcaatt ttccactgag
     1141 taatgacgcg tgtagagttt cagttcggcc tgcttatcgc gaaatccgcg taaactgtta
     1201 ttgctatgaa atttccaggc atctttaaag acctgaaaga tatagacatc ctcaatccag
     1261 gagtcacatt ttttaagcgg gcgctttgct attatcaccc gctcatccgg atttagcatc
     1321 gccgtaaatt gtacggtgac gtcatttgtc gtgttcaggt cacattgaac atctggtaca
     1381 tcactgatat aggggtcaaa tccaaaatca gcaccacagg cacaaagacg gctaatggtg
     1441 gcggtatcga gggaaaatcc cggccatact ttgctggagg ttaagccaat atttaatgtg
     1501 acatcatatc gaagcaatcc ctcaagaccg tcagcaccga cgaacttttc cccttcatcc
     1561 agggcctggt taataagttg ctgaacaatg ccttcatccc agtatgccga tttgcagttg
     1621 gcaatcgtaa tatccacgcc tgtgaaggga tcagtaccgg caaggcttgc aaataatgta
     1681 ttaactaatt ccatttttat tatccagctt taaggtagcg tcttatacat cgggacataa
     1741 cctacatctt ccaccaaact ctgtccctgc ggcgttaaaa accactccaa caatttttgt
     1801 gtctctgacg tcgtattttc ccgcgttacc ataaaggcat cgatgatata gggatatttg
     1861 ccgttgcgaa tgttttcggc agtcggtgca ataccgttaa tcgctagcag ttttatattt
     1921 ttatcggcgt tcatttgtgt tgcgtaataa cggaaggtgt aacctattgc gttgtttgta
     1981 ttacggtatt cggcaacgac tttaatcatt ccctccatca tacttgccac ttctgtttcc
     2041 tgcggcgaga tcatgcggac atttttcatg acctgggatt gcatcaccgt ctggctacca
     2101 gagtcttccg ggcgttgcta ggtctggatc tcctgatcgt taccgccaac agtacgccag
     2161 ttggtaattg caccactgaa gatgtcacgc acctgttgtt cagtcaggga attaaccgga
     2221 ttatccgcat tgacgatgaa aacaaacgct tcacgggcaa aaggcgtgta cagcaaagta
     2281 atgcccgatt cctcagcgcg ttttttctgc ccaccggaag gttgcgccac aaaaataata
     2341 tcagcatcac ctttaacaat cctgttatac gcatcggggg tacgagagct ctccagatat
     2401 tccctggtgt gaaaatcttc cggtataacg ctcaaagcat aaaatgcaga ggcataaatt
     2461 gggtacgccg ccgtagcacc atcgagtcgc ggccagtttt gcgtaaactg aatttgcggt
     2521 ttaccacgca gtggtgtcag ttgattattg agtttatcgg gtcgccaggc ccaggtatca
     2581 atattttcgc gcacaccgac gccgggattg agctgtttat ataaataacc ctgataacca
     2641 gcgacaaaac caagcatgag aaggataaac aggagggaat tacggcaacg tagcccggat
     2701 gtaccggtta aaggatgtct ggttggccgg ccgtacccca gcgaaaaact gatatgcgcg
     2761 ccaacgggta taatgactat ccaccagtac tcacctccga ctactgagac taacaaaacg
     2821 cccagccagg ggagcataag atcagccaac caacccgatc ccatctggtg aaattgataa
     2881 tctgccagat gcagcgcgat tatccagcca accaggatta ataaaccggg tactaaaaaa
     2941 agacattgtc gccagacggc tatttctctt ttttctggtg cactatcgcc tgcccatact
     3001 cgccccgaga aaaatagcat taatgcgccc agcgccagca taaaaatgtt gagcagaagg
     3061 taacttgttg gcgtgataag gatattacaa acttcaataa taccggcaag tccgacaccc
     3121 agcatagcaa caaccgccgc caaaaattgc gtcagtatgg ggatgccgaa aaaagtcatt
     3181 accagcgagg acaaaatcca ttttctgttt tgcattattc tttccattct ttttgaatgg
     3241 tgaaattata ctccccgagt ccccttgccc cttctggaca cttttccgaa atgatggcgg
     3301 aaaaaaacgg gaccctttg
//
LOCUS       DADLFA010000262         3283 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000262 DADLFA010000000
VERSION     DADLFA010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.264"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(44..991)
                     /gene="icsP"
                     /locus_tag="JQH65_003893"
     CDS             complement(44..991)
                     /gene="icsP"
                     /locus_tag="JQH65_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HAY6141138.1"
                     /translation="MKLKFLVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGNRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVRSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(2372..2524)
                     /locus_tag="JQH65_003894"
                     /pseudo
     CDS             complement(2372..2524)
                     /locus_tag="JQH65_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2580..3272
                     /gene="ospZ"
                     /locus_tag="JQH65_003895"
     CDS             2580..3272
                     /gene="ospZ"
                     /locus_tag="JQH65_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
                     /protein_id="HAY6141139.1"
                     /translation="MISPIKNIKNVFPINTANTEYIVRNIYPRVEHGYFNESPNIYDK
                     KYISGITRSMAQLKIEEFINEKSRRLNYMKTMYSPCPEDFQPISRDEASIPEGSWLTV
                     ISGKRPMGQFSVDSLYHPDLHALCELPEISCKIFPKENSDFLYIIVVFRNDSPQGELR
                     ANRFIELYDIKREIMQVLRDESPELKSIKSEIIIAREMGELFSYASEEIDSYIKQMND
                     RFSQIKARMSVT"
BASE COUNT         1035 a          605 c          577 g         1066 t
ORIGIN      
        1 gcaattttta gccatgaaaa cgtaatcaac tcggtgaacc aattcaaaaa atatacttta
       61 tacctgcgga agtgagaaaa ccaatatatt caatacttgc actattttta atggtgccag
      121 atatattggt actttgctca taaagagatg tatcaccttt tttattagaa atgtaatttc
      181 tggagccttc tatataaaat tttgcactgg gggtgacata gtatccaata ttcgcagcaa
      241 cagagcagta attttggttt tttatttcat ctttaaaaat tgtctcagtc tgatagtgtt
      301 tatctgtatc agatgaacga acccaaccac tatatttcag ttctgctcca aactcaaaat
      361 tctcatggcg gtaattagca gttaatccaa tataaggtat tttgaagagc tgtttgtaac
      421 ctattgttct ttcaccacta ggatgtgccc cttttttatt cctgttcccc ccgttttcac
      481 tataaatata actccctccc attgcattaa aactgtaacg actctcttgg taacctgcta
      541 ttagtccaag tcgataatcc aaattattta ataaccaacc tttaagattc agctcgaatt
      601 cgttagcatc acggagatgc gtattgggat gccagctctg gtcggtccat acctgagggg
      661 tattggaatt gttccagtct ttatcaacca tgcttgcttt ctggttacct aaagtagtcc
      721 aaccggaaac tccgaaagac acttttggaa ttagcttcca atcgatgcca cctctaacaa
      781 tagtcgcatt actgtatttc cagtccagtt gactaatttt tctcccccct tccttgggat
      841 gataaacgcg ttcttttgtt ttgccactca gagatcctag actaatatcc gtactgatgt
      901 tatccggtat gaaaagtgga tagttagtgg tagcatgtgt agtaaagatc gcaggtacac
      961 aaagtgcaag tacaaggaat tttaatttca tcgagaactc tacttttttg gttgaaatgt
     1021 ccatgatctt cttactttat aagataaacg tcaatgccaa gcattatttt aattaaatat
     1081 ttgtttatgt taccaagaac atatctttta ttataatatt tattgaactt ttgtaaaaat
     1141 aaaaaaatac ctcattgtgc gaataaagta acgggggcca tagatttctc tacctacctt
     1201 ttggtgttca tacctgaggc tgatagtttc aggattccgc gacaattcta ctcaccttct
     1261 tgttctctcg ccaatatgaa atcaatcaga aaagcgttcc ataagaacaa cgataagaca
     1321 ataaattgtc atcacggctg caatagccaa cgtgcctttc agtaccaaca tgacaacctc
     1381 ctgtatttga cgtaacgtgt gctgataaat atgtatttgt atctcaactg actcaataca
     1441 acacttaagt atagtttttc agattttgtt tttcccgtga aatattatgg aaatggcaga
     1501 agcaatacat tgaattccga ttcgagtgaa tacggctagc gggcacctca ctttagcact
     1561 gaagccatca acttttattt gacttatttc aatggaaagc tttctcacta tcagtacagg
     1621 taattcccat ccttttaaag tcgagagtca gactgcgact aatacagtta aagtgaccaa
     1681 actcagttat cctctacaca ttttacctca tgtttacttg gacaattcat tggtgctttt
     1741 ataaaagaat aaatattcta ctaatttaat gcatcaccta aaatagtatt ttttataata
     1801 gaactgtatc tatagtatat tattaaagct taattattta ttatgcttta atgtggttgt
     1861 ccgattaagg actaatcctt gaaaacgccg tatagggcgg gagttcactc tattgcgata
     1921 acgtaaccgt acaatattgt gcgtttccca aattgaccac tactgaacta ttctgggatt
     1981 taaatgcaat aaaatggttg gttgaaggtc gtggtgatga tgttagtcct tttgagaata
     2041 ttccagtgcc agatcttact tataataaag aggcttggca gtttggataa gctattgtcc
     2101 ttattccact aatacaaact ttttgtaaag tatattaaat atacttcatt tcatactgaa
     2161 atccccggaa gtattcaatc aaacaattac accataaaag gaagagggag ccccaattaa
     2221 gttatcaagc atgtttatga tacctgcagg gtataaattt tctggctgca agcagacaca
     2281 tataaaacgt ctttattgat cctacccaca taatatggac gcagtcgtaa gtgaggctct
     2341 gtgagcctac ccacgtaata tggacacagg cctaagcgag gttctggttt tcaaattgtt
     2401 ccggactgag gccgccacac caacggtgcc gtcgccagcg attgtaatcg cactcgatat
     2461 aattaaacac tgttgcccgc actatttccc gatagataaa gtgttctccg tggagacatt
     2521 ccacttagga acaattatat tgttcatggt aatgtttata tttgagtata gagattaaaa
     2581 tgattagtcc catcaagaat attaagaacg tatttccaat aaatactgca aatactgaat
     2641 atatagtgag aaatatttat cctagagttg aacatggtta ctttaatgaa tcaccaaaca
     2701 tatatgacaa gaagtatatc tctggtataa ccagaagtat ggcacaatta aaaatagagg
     2761 agtttattaa tgagaaatcc agacgactta attatatgaa gacaatgtat tctccatgtc
     2821 cggaagattt tcagcctatt tccagagacg aagccagtat accggaagga agctggttga
     2881 cagttatatc tggaaaacgt cctatggggc aattttctgt ggatagtttg taccatcctg
     2941 acttacatgc attatgtgag ctcccagaga ttagttgtaa gatcttccct aaagaaaata
     3001 gtgatttttt gtatataatt gttgtattca gaaatgattc tccccaagga gaactacggg
     3061 caaatcggtt tatagaacta tatgatataa aaagagagat tatgcaagtg ttaagagatg
     3121 agtcgccaga gttaaagtct attaaatctg agattattat tgctcgtgaa atgggagaac
     3181 tttttagtta tgcatcagag gaaatagata gctacataaa acaaatgaat gacaggtttt
     3241 ctcaaatcaa ggctcgaatg tctgttactt aatgtatatt aat
//
LOCUS       DADLFA010000263         3247 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000263 DADLFA010000000
VERSION     DADLFA010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.265"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            95..1246
                     /gene="yiaY"
                     /locus_tag="JQH65_003896"
     CDS             95..1246
                     /gene="yiaY"
                     /locus_tag="JQH65_003896"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HAY6141140.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAYQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            1411..2949
                     /locus_tag="JQH65_003897"
     CDS             1411..2949
                     /locus_tag="JQH65_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HAY6141141.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
BASE COUNT          794 a          800 c          876 g          777 t
ORIGIN      
        1 agattaattt tgataattaa taataatgat catgttttcg aatgaaaatc ctcagtaagc
       61 ttcccaccct tttttacact ttcaggagtg tgttatggca tcttcaactt tctttattcc
      121 ttctgtgaat gtcatcggcg ctgattcatt gactgatgca atgaatatga tggcagatta
      181 tggatttacc cgtaccttaa ttgtcactga cagtatgtta acgaaattag gtatggcggg
      241 cgatgtgcaa aaagcactgg aagaacgcaa tatttttagc gttatttatg atggcaccca
      301 acctaacccc accacggaaa acgtcgccgc aggtttgaaa ttacttaaag agaataattg
      361 cgatagcgtg atctccttag gcggtggttc tccacacgac tgcgcaaaag gtattgcgct
      421 ggtggcagcc aatggcggcg atattcgcga ttacgaaggc gttgaccgct ctgcaaaacc
      481 gcagctgccg atgatcgcca tcaacaccac tgcgggtacg gcctctgaaa tgacccgttt
      541 ctgcatcatc actgacgaag cgcgtcatat caaaatggcg attgttgata aacatgtcac
      601 tccgctgctt tctgtcaatg actcctctct gatgattggt atgccgaagt cactgaccgc
      661 cgcaacgggt atggatgcct taacgcacgc tatcgaagca tatgtttcta ttgccgccac
      721 gccgatcact gacgcttgtg cactgaaagc cgtgaccatg attgccgaaa acctgccgtt
      781 agccgttgaa gatggcagta atgcgaaagc gcgtgaagca atggcttatg cccagttcct
      841 tgccggtatg gcgttcaata atgcttctct gggttatgtt catgcgatgg cgtaccagct
      901 gggcggtttc tacaacctgc cacacggtgt atgtaacgcc gttctgctgc cccatgttca
      961 ggtattcaac agcaaagtcg ccgctgcacg tctgcgtgac tgtgccgctg caatgggcgt
     1021 gaacgtgaca ggtaaaaacg acgcggaagg tgctgaagcc tgcattaacg ccatccgtga
     1081 actggcgaag aaagtggata tcccggcagg cctacgcgac ctgaacgtga aagaagaaga
     1141 tttcgcggtt ctggcgacta atgccctgaa agatgcctgt ggttttacta acccgatcca
     1201 ggcaactcac gaagaaattg tggcgattta tcgcgcagcg atgtaatcat catttccaca
     1261 acggctggca aattgtcagc cgctttttca actatctctg taacccttgc ccgtaaattc
     1321 gtgatagccg tcgtaaagct gttaccgact ggcgaagatt tcgccagtca cgtctaccct
     1381 tgttatacct cacaccgcaa ggagacgatc atgaccaata atcccccttc agcacagatt
     1441 aagcccggcg agtatggttt ccccctcaag ttaaaagccc gctatgacaa ctttattggc
     1501 ggcgaatggg tagcccctgc cgatggtgag tattaccaga acctgacgcc ggtgactggg
     1561 cagctgctgt gcgaagtggc gtcttcgggc aaacgagaca tcgatctggc gctagatgct
     1621 gcgcacaaag tgaaagataa atgggcgcac acctcggtgc aggatcgcgc ggcgattctg
     1681 tttaagattg ccgatcgaat ggaacaaaac ctcgagctgt tagcgacagc tgaaacctgg
     1741 gataacggca aacccattcg cgaaaccagt gctgccgacg tgccgctggc gattgatcat
     1801 tttcgctatt tcgcctcgtg tattcgggcg caggaaggtg ggatcagtga agttgatagc
     1861 gaaaccgtgg cctatcattt ccatgaaccg ttaggcgtgg tggggcagat tatcccgtgg
     1921 aacttcccgc tgctgatggc gagctggaaa atggctcccg cgctggcggc gggcaactgt
     1981 gtggtgctga aacccgcacg tcttaccccg ctttctgtac tgctgctaat ggaaattgtc
     2041 ggtgatttac tgccgccggg cgtggtgaac gtggtcaatg gcgcaggtgg ggaaattggc
     2101 gaatatctgg cgacctcgaa acgcatcgcc aaagtggcgt ttaccggctc aacggaagtg
     2161 ggccaacaaa ttatgcaata cgcaacgcaa aacattattc cggtgacgct ggagttgggc
     2221 ggtaagtcgc caaatatctt ctttgctgat gtgatggatg aagaagatgc ctttttcgat
     2281 aaagcgctgg aaggctttgc actgtttgcc tttaaccagg gcgaagtttg cacctgtccg
     2341 agtcgtgctt tagtgcagga atctatctac gaacgcttta tggaacgcgc catccgccgt
     2401 gtcgaaagca ttcgtagcgg taacccgctc gacagcgtga cgcaaatggg cgcgcaggtt
     2461 tctcacgggc aactggaaac catcctcaac tacattgata tcggtaaaaa agagggcgct
     2521 gacgtgctca caggcgggcg gcgcaagctg ctggaaggtg aactgaaaga cggctactac
     2581 ctcgaaccga cgattctgtt tggtcagaac aatatgcggg tgttccagga ggagattttt
     2641 ggcccggtgc tggcggtgac caccttcaaa acgatggaag aagcgctgga gctggcgaac
     2701 gatacgcaat atggcctggg cgcgggcgtc tggagccgca acggtaatct ggcctataag
     2761 atggggcgcg gcatacaggc tgggcgcgtg tggaccaact gttatcacgc ttacccggca
     2821 catgcggcgt ttggtggcta caaacaatca ggtatcggtc gcgaaaccca caagatgatg
     2881 ctggagcatt accagcaaac caagtgcctg ctggtgagct actcggataa accgttgggg
     2941 ctgttctgat ataagaagct ggtcgcattg ggtattcatt gcctgatgtg acgcttacgc
     3001 gtcttatcat gcctacggga acctgaccgt aggccggata aggcgtacac gccgcatccg
     3061 gcataaacaa agcgcacgtt gttacaattt acgttttaaa cctacgtgcg caataatcca
     3121 gactacgcca acagaagtgc gtttctgggg cgtgttttgt caggagttta atctcgcgtt
     3181 tcgatggtga gcgggcgaac taggccgtaa atcaccattg gtgatccgag gccaatcagc
     3241 aataaac
//
LOCUS       DADLFA010000264         3227 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000264 DADLFA010000000
VERSION     DADLFA010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.266"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..1010)
                     /locus_tag="JQH65_003898"
     CDS             complement(<1..1010)
                     /locus_tag="JQH65_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HAY6141142.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTDHSEKYSMLSGMRAHILRR
                     DNNYQFIVTINSKNYGCSLDNTDVNWCSVVYLLNNMTVNDNANDVAVTESYKPIWNWK
                     ISQYNVFDIKFETIINPQFADRIYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GYLGIMTEGENFLCQLLTIEDEVLNVLLRENFNEQLDTNINTIISNLNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NG"
     gene            complement(1272..2862)
                     /locus_tag="JQH65_003899"
                     /pseudo
     CDS             complement(1272..2862)
                     /locus_tag="JQH65_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
BASE COUNT         1034 a          591 c          507 g         1095 t
ORIGIN      
        1 ttaccattgc aattcgtttc atgatttcca gctaatacat gaacattttc attgatactc
       61 attatgcgtt ttaacaaatc gatgataaat tgatcgccac agatattact aaatcgatcg
      121 ccggtttggt cgcccagaaa cgtacatggc gtgactgcgt ctttattaat aactaaataa
      181 ggctgaagac tttctaaaac attatctctt ctgttcaggt tactgattat tgtattaata
      241 ttagtgtcaa gttgctcatt aaagttttcc cttagcaata cgttgagaac ttcatcttct
      301 atagtaagca actggcaaag gaaattttct ccttcagtca tgattcccaa atatcccgta
      361 gcaaaaagcg cgtacaaaac tgcaccaact gaaccatcgg tatccccaaa ataggtcggc
      421 ctggtacttg tgggatcaac aggtgaacaa ttggagaaat agattctgtc agcgaactgg
      481 gggtttataa ttgtttcgaa tttaatatca aatacattgt attgcgatat tttccagttc
      541 cagatgggtt tgtacgactc agtaactgca acatcattag cattatcatt tactgtcata
      601 ttattaagta aataaacaac actgcaccaa ttaacatcag tattatcaag agagcaaccg
      661 taatttttac tattaattgt cacaataaac tgataattat tatccctacg tagaatatga
      721 gcacgcatgc ccgatagcat actatatttt tctgaatgat ccgtttgtat ggtaaatgaa
      781 attttgtcat cttgaataga ttgttggaac cgttctattt gccaatcatc tattttttcc
      841 acaacctcac cgagcattgt cctggcaata cttgatatat catcacctat accatttgca
      901 atatacccaa acagttcttt agtgaagttg gtaacatgct cttgcaccat atcatttgtt
      961 gcgattgtcg ttgtactgtc gttagtgcct gccacagttc caattttcat cttttacctc
     1021 atattattca ccgcgaggaa tacatagtta acattttatc ttatgacata cacctcatgt
     1081 tcgatggaaa aataattata ttttgcacaa atcattcagc cttaaagcat aatatgaaat
     1141 ttcttatttc atagcagaga aagtccctaa agattgtaga ggcgtcatca gaacatgtct
     1201 ctacaatcga ttaagaaaac ctatgaaata ctggcaggat taaaaagata taccacagca
     1261 ctcatggagt tttaatgtgg ttgaaaacaa ctaagccctg ggacaccttt tttatcacca
     1321 cgttcgttag gagctatctt ccaggaattt agccccatgg tataattatc aattttttga
     1381 tgctgatcta cccctaatcc ataatggcca tatttctgtt tagttttttt gagcgtctgt
     1441 ccacatttga agatatcttc aggttttggt ctgaaagcag tagtgttatt aaaataattg
     1501 tcatatacag gtgtggatgc tatagcattg cttctaatta gatggataaa acgatcatcc
     1561 atgtgttgtt tatgtttttt attaaatata tttgctaatt cctcaggatc aattgggtct
     1621 ttcatctgat taaatggaac ctgcaaagca cctagacaat aacattcatt ttcttcattt
     1681 cttattattc cgtgatgact gcttaaaact ttcgtttctg agtcataatt acaaacatcg
     1741 agcgttttaa ttaagttata cgtatcaccg gcagagagtt tatgatttgc tagtcgtgca
     1801 gtatagttac cattgaaatt gatttcataa tttccagcca gaacgacgtc atttttattt
     1861 atacgactat ctttattact ctccatattc cgcatagaat ttagcaaagt aaggatatat
     1921 ttatcaccaa aaatagtact gaatctatcg cctgtatgat ccccaagaaa gatacaaggg
     1981 gtaatcgcat cgccttttaa aaaaacatgt tgatgcaact tattcaagat catgtctctc
     2041 gtatctaata aagtaaagag tttttccaat tttttatttt catatgcgaa tgaagccatg
     2101 tcttcgtgtt ttaataattc acataataaa ctccacccct tttccctgat acctatatgt
     2161 cctgaaacga gcaatgcata aagtacagcg ccaactcttc catctgtatc gccaaaataa
     2221 gccgtgttgg gattttgtgg gttaatcaca ccacactgtt tataaaaatc tatttttgaa
     2281 aacacagaat taacatagct agcaaactta tccttaggaa acgtctcttt agagacatcc
     2341 caacgctagt cagctttaaa attaccgcta agaagagtaa agaaattttc aggtatgata
     2401 gaaaggccat taactttata aattatgtct gatttatctc cggagaaact aataaaataa
     2461 taaccatcct tacactcaat cgtaacggta atgggagacg acaattttat tacatggtta
     2521 ttgtctggta taattgtaaa gataaacgtg tcagtagtat tatcggcgaa actattatat
     2581 tcttgaatat ctatagatgg ctcattattg taaaaccata tttttttcta tctgagaact
     2641 atattcattt accttccagc gaccatgttg caggaaatag atgatatgca ggcctaaatc
     2701 gttatctatt tgcgaatgtt tatccagctt atcaatttca actatctttg aacgatgttt
     2761 tttatcaacc ttgccatggt gttgatgatt gttaacttga tctgaatgct tatcttgatg
     2821 gcttgaagag atgtgcttaa atatattgtg aaatactttc ataaataacc cgcaatattt
     2881 ataacttgtt agtaacaaaa taaacgatac atgtctgaaa gacatattta cgacgataca
     2941 aagtaataat acttatatat ccgatgattt taagcaccga gactatccgg tcttttacta
     3001 aataatggaa aacaatccat ccctggaaga taattgaaaa aaacaacaga ataagaatta
     3061 atcttaggat aaacatttat ttatcatggc cttttgaacg ctgaaaagta gcgctctgta
     3121 aagtaaaccc cgctatagtt atgctatgca tttgtcttta atgatatctc gtcattgtat
     3181 taacaggctg aaattatcat taatgcacat ttcaatatta gcaggga
//
LOCUS       DADLFA010000265         3223 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000265 DADLFA010000000
VERSION     DADLFA010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.267"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..907
                     /locus_tag="JQH65_003900"
     CDS             <1..907
                     /locus_tag="JQH65_003900"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HAY6141143.1"
                     /translation="ANIDVAGYSHESLNYADSNPGKIKFTPGGVGRNIAQNLALLGNK
                     AWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLDNTGEMLVAINDM
                     NISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVPVFVDPVSAWKCV
                     KVRDRLNQIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLNRLVLSMGGDGVY
                     YSDISGENSWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESVRFAQGCSSMALS
                     CEYTNNPDLSIANVISLVENAERLN"
     gene            874..1833
                     /locus_tag="JQH65_003901"
     CDS             874..1833
                     /locus_tag="JQH65_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HAY6141144.1"
                     /translation="MVSGERRTSELKISPELLQISPEVQDALKNKKPVVALESTIISH
                     GMPFPQNAQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEFELLGREGHNVTKVS
                     RRDLPFVVAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQEL
                     ANTNVTVVCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDS
                     ASEIARAMAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKES
                     TLFLLARVAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            1927..3176
                     /locus_tag="JQH65_003902"
                     /pseudo
     CDS             1927..3176
                     /locus_tag="JQH65_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
BASE COUNT          770 a          724 c          871 g          858 t
ORIGIN      
        1 ggcgaatatt gatgtcgccg gatattcaca tgaatcatta aattatgcgg attcaaatcc
       61 aggtaaaata aaatttacgc ctggtggagt cgggcgcaat attgcacaaa acctggcgtt
      121 gctggggaac aaagcctggc tactgagcgc cgtaggcagt gacttttatg gtcaatcgct
      181 gctaacgcaa accaatcaat ctggcgttta tgtcgataaa tgcctgattg tgccgggaga
      241 aaatacgtct agttatttat cattactcga taataccggt gaaatgctgg ttgctataaa
      301 tgacatgaat attagcaacg ctattacagc tgaatatctc gcacagcacc gtgaatttat
      361 tcagagggca aaggtcattg tcgcggactg taatatcagt gaagaggcac tggcatggat
      421 tctggataat gccgccaacg tacccgtatt tgtcgatccg gtttccgcat ggaaatgtgt
      481 caaagtgcgc gaccgtctaa atcagatcca cactctgaag ccaaaccgcc ttgaagcgga
      541 aaccctgagt gggattgcgc tgtcagggcg tgaagatgtg gcaaaagttg ctgcctggtt
      601 ccatcaacat ggcctgaacc gactggtatt gagcatgggc ggcgacggcg tttattacag
      661 cgatatcagc ggtgaaaata gctggtctgc gccgatcaaa accaatgtta ttaatgttac
      721 cggagcgggc gatgccatga tggcgggact tgcttcgtgt tgggtagacg gaatgccgtt
      781 tgccgaatct gttcgtttcg cacagggatg ttcgtcaatg gcgctctcct gtgaatacac
      841 caataacccc gatttatcga ttgccaacgt tatatcgtta gtggagaacg cagaacgtct
      901 gaattaaaaa tttcccctga attattacaa atttccccgg aagtgcagga cgctttaaaa
      961 aacaaaaaac cggttgtggc gctggaatcg accattattt ctcacgggat gccgttccca
     1021 caaaatgccc agaccgcaat tgaagtagaa gaaactattc gtaaacaggg cgctgtacct
     1081 gccactatcg ccattattgg cggcgtgatg aaagtgggtt taagcaaaga agaatttgaa
     1141 ttactgggtc gtgaagggca taacgtgacc aaagttagtc gtcgcgattt accttttgtt
     1201 gttgccgccg gaaaaaatgg cgcaaccact gtggcttcaa cgatgattat tgcggcgctt
     1261 gccggaatta aagtatttgc caccggggga attggtggtg tgcatcgcgg ggcggaacat
     1321 accttcgata tttctgccga tttgcaagaa ctggcaaata ctaatgtcac cgttgtttgt
     1381 gccggggcga aatctattct cgatttagga ttaaccactg agtatttaga aaccttcggt
     1441 gtgccgttaa ttggctatca gactaaagct ctgcctgcgt ttttctgccg taccagctcg
     1501 tttgacgtca gtattcgtct cgacagcgcc agtgaaattg cccgtgcaat ggcggtgaaa
     1561 tggcaaagcg ggctgaacgg tggcctcgtg gtagcgaacc cgatcccgga acagtttgcg
     1621 atgccggagg aatctatcaa tgcagccata gatcaagccg tcgccgaagc cgaagagcag
     1681 ggcgttattg gtaaagaaag tacactgttc ctgctggcgc gcgttgctga gctgaccggc
     1741 ggtgacagcc tgaaatccaa tatccagctg gtattcaaca acgccattct ggcgagtgaa
     1801 attgccaaag aataccagcg tctcgcgggt taatttccga cgggcagaaa gccgtcgccc
     1861 gtactcatca agcataccaa aacctggcgc tgacgccggg tttactggca tgaagggaaa
     1921 atcattatgg atataatgag aagtgttgtg gggatggtgg tgttactggc aatagcattt
     1981 ctgttgtcag tgaataaaaa gagcatcagt ttgcgcacgg ttggagccgc actgctgctg
     2041 caaatcgcta ttggtggcat catgctctac ttcccaccgg gaaagtgggc agtagaacag
     2101 gcggcattag gcgttcataa agtgatgtct tacagtgatg ccggtaggcc ttcatttttg
     2161 gttcgctggt tgggccgaaa atggatgtcc tgtttgacgg tgcgggtttt atcttcgcct
     2221 ttcgcgtact tccggcgatt attttcgtta ctgcgctcat cagtctgctg tactacattg
     2281 gcgtgatggg gctgctgatt cgcatccttg gcagcatttt ccagaaagct cttaacatca
     2341 gcaaaatcga atcttttgtc gcagtcacaa ctattttcct cgggcaaaat gagatcccgg
     2401 cgatcgttaa accgtttatc gatcgcatga atcgcaacga gttgtttacc gcaatttgta
     2461 gcgggatggc gtccattgct ggttcgatga tgattggtta tgccggaatg ggcgtaccaa
     2521 ttgactacct gttagcggca tcgctgatgg cgatcccagg tggtattttg tttgcacgta
     2581 ttcttagccc ggccaccgag ccttcgcagg tcacatttga aaatctgtcg ttcagcgaaa
     2641 cgccgccaaa aagctttatc gaagcggcgg cgagcggtgc gatgaccggg ctgaaaatcg
     2701 ccgctggtgt agcgacggtg gtaatggcat ttgtcgcaat tattgcgctg atcaacggca
     2761 ttatcggcgg aattggtggc tggtttggtt tcgccaatgc ctctctggaa agtatttttg
     2821 gctatgtgct ggcaccgctg gcgtggatca tgggtgtgga ctggagtgat gccaatcttg
     2881 cgggtagcct gattgggcag aaactggcga ttaacgaatt cgtcgcttac ctgagtttct
     2941 ccccatacct gcaaacgggc ggcacgctgg aagtgaaaac cattgcgatt atctcctttg
     3001 cgctttgtgg ttttgctaac tttggttcta tcggtgttgt cgttggcgca ttttcggcta
     3061 tttcgccaaa acgcgcgccg gaaatcgccc agcttggttt acgggcgctg gcagcagcaa
     3121 cgctttccaa cctgatgagt gcgactattg ccgggttctt tattggttta gcttgagttt
     3181 cattgccgga tgtacgcgct tattaagcgt tgcatccggc aat
//
LOCUS       DADLFA010000266         3195 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000266 DADLFA010000000
VERSION     DADLFA010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.268"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            199..939
                     /locus_tag="JQH65_003903"
     CDS             199..939
                     /locus_tag="JQH65_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HAY6141145.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(910..1677)
                     /locus_tag="JQH65_003904"
     CDS             complement(910..1677)
                     /locus_tag="JQH65_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HAY6141146.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(1883..2461)
                     /gene="lpcA"
                     /locus_tag="JQH65_003905"
     CDS             complement(1883..2461)
                     /gene="lpcA"
                     /locus_tag="JQH65_003905"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HAY6141147.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            2701..>3195
                     /locus_tag="JQH65_003906"
     CDS             2701..>3195
                     /locus_tag="JQH65_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HAY6141148.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFF"
BASE COUNT          765 a          810 c          819 g          801 t
ORIGIN      
        1 atcttaggaa ataaatgata catgcgcttt tcggactatt gaaaactgcc cctgtgctaa
       61 atttgtaaac cctgcgccga caggcttgcg taaaactctt ttggtgataa tgtgagcctt
      121 ccaaatccag actattccga tttgggatcc attttgccgt cctggcgtgt gtaaccgttt
      181 tatcaaggaa taagcagtat gcgtaaaatc gcattaattc ttgcgatgtt gttgatcccg
      241 tgcgtttcgt ttgccggttt gctgggcagc agtagctcga ccacgccagt gagcaaagag
      301 tataagcagc agttgatggg atcccctgtc tacatccaga tcttcaagga agaacgtacg
      361 ctcgatctct acgtcaaaat gggcgagcaa tatcagctgc tcgacagtta taaaatctgt
      421 aaatattccg gcggcttagg gccaaaacag cgtcagggcg atttcaaaag cccggaaggg
      481 ttttatagcg tccagcgtaa tcagttaaaa ccagacagcc gttactacaa agcgattaat
      541 attggtttcc ccaatgccta tgaccgtgcg catggttatg aagggaaata cctgatgatc
      601 cacggcgatt gtgtttccat cggctgctac gcaatgacca atcagggtat tgatgagatc
      661 ttccagttcg ttactggtgc gctggtgttt ggtcagccga gcgtgcaagt gagtatttac
      721 ccgttccgca tgaccgacgc caatatgaag cgccataaat attccaactt taaggacttc
      781 tgggagcaac tgaagccggg ttacgactac tttgagcaaa cccgtaagcc accgaccgtt
      841 tctgtggtca atggtcgtta cgtggtcagc aagccgttaa gccacgaagt tgtgcagcca
      901 caactggcat caaactacac gctccccgag gcaaaataag cgccattcgc ctggcataat
      961 cttttgccag gtttcattgc ccgtcagcgg ctgtgtcgca atcaccgtga ccacatcatt
     1021 cggtgtggtc tgcgagctga agtcgatttc tacatcctga tccagcagcg ttgccacgcc
     1081 aaacggtgcg cggcgggtga tccagtgcag attcgtcgag caatacgcca ttacatagcg
     1141 cccatccgaa agcagcatgt tgaaaacgcc cttctgccgc agttcgtccg ccagtgaagc
     1201 gatatattta aacactgctg ccatgttgcc cggcgtgcgc gggtaacgct gcgttaattt
     1261 atgcagcagc cagcagaagg ctttttcgct gtcggtttcg cctaccgggc ggaagttgcc
     1321 ggtttccagt gatttgtagc ccgtcagttg tccgttatgg gcataagtcc agttacgccc
     1381 ccataactcg cgggtaaatg ggtgagtatt ttccaacgcc acctcgcccc gattagcctg
     1441 gcgaatatga gccaccaccg aacaggattt tatcgggtag tcctggacaa gtttggcgat
     1501 gggggaatta aagctgggtt gtggatcttt aaatgtgcga cagcctttac cttcgtaaaa
     1561 ggtaatgccc cagccatctt tatgtggccc ggttccacca ccacgctgta caagtccggt
     1621 gaaactaaag cagatatcgg tagggacgtt ggcgctcatc ccgagcaatt cgcacatggg
     1681 taacctccac aacagcggga gcgcatggcc cccggcgaag attgatttaa gaattttcag
     1741 gtcggatgcg gcgtaaacga attattcggc ctacggttcg cgtacagatt tgtaggcatg
     1801 ataagacgcg tcagcatcgc atcaggcatc agcgcacaag tgccggatgc ggcgtaaacg
     1861 ccttatccgg cctacgccag acttacttaa ccatctcttt ttcaattaac tggatcagga
     1921 tatggatcac tttaatgtga atctcctgaa tgcggtcggc ataaccaaag tgcggtacgc
     1981 gaatttcgat atccgccgtg ccagccattt tgccgccgtc tttaccggtc agggtgatca
     2041 ctttcatccc cttctcacgc gccgctgcga tcgctttgat cacgtttgca gagttaccgg
     2101 aggtggagat ccccagcagt acatcgcctt cgcgacctac cgcttcaacg tagcgggaga
     2161 aaatatcatt gaaaccgaaa tcattaccga cgcaggaaat atgactaacg tcagaaatag
     2221 caatcgccgg gtagcccgga cggttttcac ggtagcgacc ggtcaactct tcggcaaagt
     2281 gcatagcgtc gcaatgggaa ccgccgttgc cgcaggaaag cactttgcca ccggctttaa
     2341 agctgtctgc taacaggacc gccgcgcgct gaatggcgtg aatattggcg tcatctttta
     2401 aaaagttagc cagcgtttcc gccgcttcgt tcagttcgtt acgaataaga tcttggtaca
     2461 tgaggatatc cttcagcata aatgtaatag acaaaatgca gtgtaccgga taccgccaaa
     2521 agcgagaagt acgggcaggt gctatgacca ggactttttg acctgaagtg cggataaaaa
     2581 cagcaacaat gtgagctttg ttgtaattat attgtaaata cattgctaaa tgtatttaca
     2641 tccactacaa ccatatcatc acaagtggtc agacctccta caagtaaggg gcttttcgtt
     2701 atgatgattt tgagtattct cgctacggtt gtcctgctcg gcgcgttgtt ctatcaccgc
     2761 gtgagcttat ttatcagcag tctgattttg ctcgcctgga cagccgccct cggcgttgct
     2821 ggtctgtggt cggcgtgggt actggtgcct ctggccatta tcctcgtgcc atttaacttt
     2881 gcgcctatgc gtaagtcgat gatttccgcg ccggtatttc gaggtttccg taaggtgatg
     2941 ccgccgatgt cgcgcactga gaaagaagcg attgatgcgg gcaccacctg gtgggagggc
     3001 gacttgttcc agggcaagcc ggactggaaa aagctgcata actatccgca gccgcgcctg
     3061 accgccgaag agcaagcgtt tctcgacggc ccggtagaag aagcctgccg gatggcgaat
     3121 gatttccaga ttactcatga gctggcggat ctgccgccgg agttgtgggc gtaccttaaa
     3181 gagcatcgct tcttc
//
LOCUS       DADLFA010000267         3172 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000267 DADLFA010000000
VERSION     DADLFA010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.269"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            345..504
                     /locus_tag="JQH65_003907"
                     /pseudo
     CDS             345..504
                     /locus_tag="JQH65_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(722..2812)
                     /gene="iha"
                     /locus_tag="JQH65_003908"
     CDS             complement(722..2812)
                     /gene="iha"
                     /locus_tag="JQH65_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HAY6141149.1"
                     /translation="MRITTLASVVIPCLGFSASSIAAAEDVMIVSASGYEKKLTNAAA
                     SVSVISQEELQSSQYHDLAEALRSVEGVDVESGTGKTGGLEISIRGMPASYTLILIDG
                     VRQGGSSDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKN
                     ADKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQVRGSTQQRQGSSVTSL
                     SDTAATRIPYPTESQNYNLGARLDWKASEQDVLWFDMDTTRQRYDNRDGQLGSLTGGY
                     DRTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKRDKWGLAGQPRE
                     LKESNLILNSLLLTPLGESHLVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDE
                     WHLTDALALTAGSRYEHHEQFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLH
                     KGISGVSGQGKTNLLGNPDLKPEESVSYEAGVYYDNPAGLNANVTGFMTDFSNKIVSY
                     SINDNTNSYVNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSY
                     TPEHMVNAKLNWQITEEVASWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAW
                     TVVDAGLSWKMTDALTLNAAVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYV
                     IPERNYWMSLNYQF"
BASE COUNT          795 a          836 c          732 g          809 t
ORIGIN      
        1 gacctctcag taaatatcaa tacggttctg gcgagccgct tacgtgcagc agtcttatcc
       61 ggcagtttaa aggggaggac ggagaccaat gaggcactgg agcagattca gtgcagaggt
      121 gacctgcttt atcaaactta attgtgacaa aatggcgatt gagaaggtgc aattgactta
      181 tccggattcg gtcgtggcga ggtggtgccc ctgcattctg ctaaccgggt tatgagccag
      241 gagtcagcga tagacaaagg gacttatctg accgggcccc cggaaaaatg gcgggagaga
      301 atgatacctt cctgctgacg agaccggact ttgagatgac gagcgatagt gcggcagtga
      361 tgtacagcct gatcggtagc tgcaaaactt aacggaatcg agccggaaac gtggctacgc
      421 cacgtgatca gtgtaatcaa cacctggcct gccaaccgca tgaaagagtt gttgccctgg
      481 aacgtcactc tctctgtaaa ctaattttta ccccacgtcc ttcacggtgc gcttacactc
      541 tggacaagta tgtaaatgat aagttaaaaa taatccgtgc atttcctgtg acgatattag
      601 ttgttggtaa cgaaatctca ttagcggatc ggaaatgcac ttaacctaat acgttgccac
      661 tgttccgcca ggagttgcag tggcaacgta ttctaccgtc agtgatagcg ttttgttatt
      721 atcagaactg atagttcagc gacatccagt aatttcgctc aggtatcaca tatcctgttg
      781 ttgatgatcc cgtctggaag taatcaccgg catacagcgt actcttaccg gcactgtaca
      841 ggctcacgtc actgtaatcc ttgttgagca ggttattcac cgcagcattc agcgtcaggg
      901 catccgtcat cttccaggac agacctgcat ccaccaccgt ccaggctttc aggtattctc
      961 ctttctcatc atacactttc ttctgtacag cgctcagtga cgaataattc tgggtgaaac
     1021 gtggtgtttt cccgcgataa cgggcaccca gccatgatgc cacctcttcg gtgatctgcc
     1081 agttcagttt cgcattcacc atgtgttcag gggtataact cagcggcgca cctttgttat
     1141 caccatcacg ttgttcactt cgggtccagg tgtaattcag tgacagcgtg acatcctctg
     1201 accacagcgg caatgtgccg gcaaattcca caccgtgcaa ccgggccttt ccgctgttta
     1261 catagctatt ggtgttatca tttatggaat aagagacaat cttgttggag aagtcagtca
     1321 taaaacctgt gacattggca ttcagaccgg cggggttatc gtaatacacc ccagcctcat
     1381 aactgacgct ctcttccggc ttcaggtcgg ggttaccaag tagatttgtt tttccctgcc
     1441 cggacacacc actaatccct ttatgtagct gccccattct gggtgcctta tatcccgtgg
     1501 tcacaccgcc tttcagcgtc caggcatctg ccacatccca gaccagatat gcacgcggac
     1561 tgaagtgtcc cccgaattgc tcatgatgtt catagcggct gcccgcagtc agcgcaagtg
     1621 catccgtgag atgccactca tcctcagcaa ataccgacca gcttttctgc cggaaagttt
     1681 cacctgtgct ggcaaggaca actccgtctt tcatggacga gctctgaaac tcgcccccca
     1741 ccgtaaccag atgagattct cccagagggg taagcagtaa tgaattcagg ataaggttcg
     1801 attccttaag ctcccgcggc tgaccggcaa gcccccattt gtcgcgcttc agtacactgc
     1861 gtacgagctc acgaccttta ttttctgtct cgttccagtt cagatacgat ttccatgttc
     1921 cgaaggtgaa agtatgatca tagccagctg aaattttgtt ccgctcatag cgcagggtcc
     1981 ggtcatatcc ccccgtcaga ctccccagtt gcccatcccg gttatcataa cgctgccggg
     2041 tggtatccat atcaaaccag agcacatcct gctccgacgc cttccagtca agacgtgcac
     2101 caagattata attctgtgac tccgtgggat aaggaatacg cgtggctgct gtatcgctca
     2161 gtgatgtgac cgatgagccc tgacgctgtt gtgtgctacc gcgtacctgc aggctgacag
     2221 aatcatccac aagggggcca ctgctccaga aattaaactg gctgctgtta ccccatttgt
     2281 tgctttcctg cagattcagc cctgcattga cggaagagag ccatttgtct gcattctttc
     2341 tggtaatgat attcaccaca ccgcccatcg catcagagcc atacagtgtg gacatcggcc
     2401 ccctgataac ctcaatacgc tcaatggcgg ccagaggggg catgaacccg gtattcatgg
     2461 cagaaaaacc gttgggagtc acgtcactgc ttccgccctg acgaacacca tcaatcagta
     2521 tcagcgtgta actggctggc attcctcgga tgctgatttc cagcccaccg gttttacccg
     2581 taccactttc aacatccaca ccctctactg atctcagagc ctccgccaga tcgtggtact
     2641 ggctggactg caattcctcc tggctaatca cagaaacact ggcggctgcg ttagtcagct
     2701 ttttctcata gccggatgcc gagacaatca tcacatcctc tgcagcagct atgctgctgg
     2761 ctgaaaatcc gagacaggga atgactacgg aagccagagt ggttattcgc atattactaa
     2821 tctccgatca tgttaaccgg caagacaata cacaaataca aacaataatg agaataattt
     2881 atatttgcct tttacaaaaa ataaagatta ttaacaaaac taaatataaa taacaactgc
     2941 ctcggcatgc cagttaacag ctatccgtat ggcgtgttcg gttttgcgaa taaaaaatgg
     3001 ttactgtaga agcggatttc caccacatgt caaccatgaa tggtcagtgg tgcgagacga
     3061 ctgggatgat ttggtcgatg acttcttccg gtgaatactg atagaaacat tgaaatattg
     3121 taagagaaag cgttcaaaat tctgtggcac gtgaaaaatt ctacagcgtc at
//
LOCUS       DADLFA010000268         3166 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000268 DADLFA010000000
VERSION     DADLFA010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.270"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            93..3023
                     /locus_tag="JQH65_003909"
     rRNA            93..3023
                     /locus_tag="JQH65_003909"
                     /product="23S ribosomal RNA"
     gene            3101..>3166
                     /gene="rrf"
                     /locus_tag="JQH65_003910"
     rRNA            3101..>3166
                     /gene="rrf"
                     /locus_tag="JQH65_003910"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          847 a          693 c          976 g          650 t
ORIGIN      
        1 tcaagctgaa aattgaaaca ctgaacaacg aaagttgttc gtgagtctct caaattttcg
       61 caacacgatg atgaatcgta agaaacatct tcgggttgtg aggttaagcg actaagcgta
      121 cacggtggat gccctggcag tcagaggcga tgaaggacgt gctaatctgc gataagcgtc
      181 ggtaaggtga tatgaaccat tataaccggc gatttccgaa tggggaaacc cagtgtgatt
      241 cgtcacacta tcattaactg aatccatagg ttaatgaggc gaaccggggg aactgaaaca
      301 tctaagtacc ccgaggaaaa gaaatcaacc gagattcccc cagtagcggc gagcgaacgg
      361 ggagcagccc agagcctgaa tcagtgtgtg tgttagtgga agcgtctgga aaggcgtgcg
      421 atacagggtg acagccccgt acacaaaaat gcacatattg tgagctcgat gagtagggcg
      481 ggacacgtgg tatcctgtct gaatatgggg ggaccatcct ccaaggctaa atactcctga
      541 ctgaccgata gtgaaccagt accgtgaggg aaaggcgaaa agaaccccgg cgaggggagt
      601 gaaaaagaac ctgaaaccgt gtacgtacaa gcagtgggag cacgcttagg cgtgtgactg
      661 cgtacctttt gtataatggg tcagcgactt atattctgta gcaaggttaa ccgaataggg
      721 gagccgaagg gaaaccgagt cttaactggg cgttaagttg cagggtatag acccgaaacc
      781 cggtgatcta gccatgggca ggttgaaggt tgggtaacac taactggagg accgaaccga
      841 ctaatgttga aaaattagcg gatgacttgt ggctgggggt gaaaggccaa tcaaaccggg
      901 agatagctgg ttctccccga aagctattta ggtagcgcct cgtgaattca tctccggggg
      961 tagagcactg tttcggcaag ggggtcatcc cgacttacca acccgatgca aactgcgaat
     1021 accggagaat gttatcacgg gagacacacg gcgggtgcta acgtccgtcg tgaagaggga
     1081 aacaacccag accgccagct aaggtcccaa agtcatggtt aagtgggaaa cgatgtggga
     1141 aggcccagac agccaggatg ttggcttaga agcagccatc atttaaagaa agcgtaatag
     1201 ctcactggtc gagtcggcct gcgcggaaga tgtaacgggg ctaaaccatg caccgaagct
     1261 gcggcagcga cgctgatgcg ttgttgggta ggggagcgtt ctgtaagcct gtgaaggtgt
     1321 gctgtgaggt atgctggagg tatcagaagt gcgaatgctg acataagtaa cgataaagcg
     1381 ggtgaaaagc ccgctcgccg gaagaccaag ggttcctgtc caacgttaat cggggcaggg
     1441 tgagtcgacc cctaaggcga ggccgaaagg cgtagtcgat gggaaacagg ttaatattcc
     1501 tgtacttggt gttactgcga aggggggacg gagaaggcta tgttggccgg gcgacggttg
     1561 tcccggttta agcgtgtagg ctggttttcc aggcaaatcc ggaaaactaa ggctgaggcg
     1621 tgatgacgag gcactacggt gctgaagcaa caaatgccct gcttccagga aaagcctcta
     1681 agcatcaggt aacatcaaat cgtaccccaa accgacacag gtggtcaggt agagaatacc
     1741 aaggcgcttg agagaactcg ggtgaaggaa ctaggcaaaa tggtgccgta acttcgggag
     1801 aaggcacgct gatatgtagg tgaagtccct cgcggatgga gctgaaatca gtcgaagata
     1861 ccagctggct gcaactgttt attaaaaaca cagcactgtg caaacacgaa agtggacgta
     1921 tacggtgtga cgcctgcccg gtgccggaag gttaattgat ggggtcagcg caagcgaagc
     1981 tcttgatcga agccccggta aacggcggcc gtaactataa cggtcctaag gtagcgaaat
     2041 tccttgtcgg gtaagttccg acctgcacga atggcgtaat gatggccagg ctgtctccac
     2101 ccgagactca gtgaaattga actcgctgtg aagatgcagt gtacccgcgg caagacggaa
     2161 agaccccgtg aacctttact atagcttgac actgaacatt gagccttgat gtgtaggata
     2221 ggtgggaggc tttgaagtgt ggacgccagt ctgcatggag ccgaccttga aataccaccc
     2281 tttaatgttt gatgttctaa cgttgacccg taatccgggt tgcggacagt gtctggtggg
     2341 tagtttgact ggggcggtct cctcctaaag agtaacggag gagcacgaag gttggctaat
     2401 cctggtcgga catcaggagg ttagtgcaat ggcataagcc agcttgactg cgagcgtgac
     2461 ggcgcgagca ggtgcgaaag caggtcatag tgatccggtg gttctgaatg gaagggccat
     2521 cgctcaacgg ataaaaggta ctccggggat aacaggctga taccgcccaa gagttcatat
     2581 cgacggcggt gtttggcacc tcgatgtcgg ctcatcacat cctggggctg aagtaggtcc
     2641 caagggtatg gctgttcgcc atttaaagtg gtacgcgagc tgggtttaga acgtcgtgag
     2701 acagttcggt ccctatctgc cgtgggcgct ggagaactga ggggggctgc tcctagtacg
     2761 agaggaccgg agtggacgca tcactggtgt tcgggttgtc atgccaatgg cactgcccgg
     2821 tagctaaatg cggaagagat aagtgctgaa agcatctaag cacgaaactt gccccgagat
     2881 gagttctccc tgactccttg agagtcctga aggaacgttg aagacgacga cgttgatagg
     2941 ccgggtgtgt aagcgcagcg atgcgttgag ctaaccggta ctaatgaacc gtgaggctta
     3001 accttacaac gccgaaggtg ttttggcgga tgagagaaag attttcagcc tgatacagat
     3061 taaatcagaa cgcagaagcg gtctgataaa acagaatttg cctggcggcc gtagcgcggt
     3121 ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagc
//
LOCUS       DADLFA010000269         3145 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000269 DADLFA010000000
VERSION     DADLFA010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.271"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            2..142
                     /locus_tag="JQH65_003911"
     CDS             2..142
                     /locus_tag="JQH65_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY6141150.1"
                     /translation="MRPPSPLSLAWRKFGSGKAVEDIVTLFTQRRPEISGFLEIFGNP
                     RS"
     gene            211..1305
                     /gene="mnmH"
                     /locus_tag="JQH65_003912"
     CDS             211..1305
                     /gene="mnmH"
                     /locus_tag="JQH65_003912"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HAY6141151.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRIDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLHAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAR"
     gene            1421..1786
                     /locus_tag="JQH65_003913"
                     /pseudo
     CDS             1421..1786
                     /locus_tag="JQH65_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(2348..2797)
                     /locus_tag="JQH65_003914"
     CDS             complement(2348..2797)
                     /locus_tag="JQH65_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141152.1"
                     /translation="MLAISSNLSKMIIFIFAIIIIVVLCVITYLYLYKDESLVSKHYI
                     NYMAIPENDGVFTWLPDFFSHVAVDISIYTNVEDDYFFSYFSLTNDDGGRFKKTLTVR
                     AREQVAKIVSKNDPDTKKVWCKYGKIPGQGDGVNLFLLVKLMLRIIL"
     gene            complement(2778..3145)
                     /locus_tag="JQH65_003915"
                     /pseudo
     CDS             complement(2778..3145)
                     /locus_tag="JQH65_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT          841 a          726 c          713 g          865 t
ORIGIN      
        1 gatgcgccct ccttcgccat tgagtctggc atggcgcaaa tttggcagcg gcaaagcggt
       61 agaagatatt gtgaccttgt ttacccagcg caggccggaa atcagcggat ttttagaaat
      121 tttcggcaac ccacgcagtt aaaaatcaag tttccttgcg cgttggcgcg taaaatagcg
      181 cgcaagctga tttgaggttc acttttaact atgcaagaga gacacacgga acaggactat
      241 cgtgccctgc tgattgctga tacgcccatt attgatgttc gcgcccctat cgagtttgag
      301 caaggcgcaa tgcccgccgc tatcaatctg ccgttaatga ataacgatga acgcgccgcc
      361 gttggcacct gctataaaca gcaagggtca gacgcggcgc tggcgctggg gcataaactg
      421 gtggcgggtg aaattcgtca gcagcgcata gacgcctggc gggcagcgtg cctgcaaaat
      481 ccgcaaggta ttctctgctg tgcccgtggc ggtcaacgct cacatattgt gcaaagctgg
      541 ttgcatgcag cggggattga ttatccgctg gtggaaggcg gttataaggc actgcgccag
      601 accgcgattc aggcgactat tgaactggca caaaaaccga tagtgctgat tggcggttgt
      661 accggcagcg gtaaaacgct gttagtgcag caacagccga acggtgtcga tctggaaggg
      721 ctggcgcgtc atcgcggttc ggcgtttggt cgcacgttac aaccacaact tagccaggcg
      781 agttttgaaa atctgctggc tgccgaaatg ctaaaaaccg acgcccgtca ggatttgcgc
      841 ctgtgggtgc tggaagatga aagccggatg atcggttcga atcacctgcc ggaatgtctg
      901 cgcgagcgaa tgactcaggc ggcgattgcg gtggtagaag atccgtttga gatccgtctt
      961 gaacgcctga acgaagagta tttcttgcgt atgcatcatg attttaccca cgcgtacggc
     1021 gacgaacagg gctggcagga atattgcgaa tacctgcatc acggactttc ggctattaag
     1081 cgtcggctgg ggttacagcg ctataacgaa ctcgctgccc agctggatac ggcgctgaca
     1141 acgcaactca ccaccggcag caccgacggt catctggcct ggctggtgcc gttacttaaa
     1201 gaatattacg acccaatgta tcgctatcag cttgagaaaa aagcggaaaa agttgtcttt
     1261 cgcggtgagt gggcggaagt ggcggaatgg gttaaggcgc ggtaatgagg gacgtatttg
     1321 tattagtcgc gacttgatct gacacatggc cttgaaaggt tgagagttac cggttttgat
     1381 atgagtgtcg aatccttatc caaaacaaga ggtaactctc atgcttcata ctgccaatcc
     1441 cgtcatcaaa cacaaagccg gtttgctcaa tctggctgaa gaactcagca acgtatcaaa
     1501 agcctgtaaa atcatgggcg tttcgcgtga tacgttttat cgttaccgcg aactggtcgc
     1561 tgaaggcggt gtggatgcgc agattaatcg tagtcgccgc gctcctaacc ttaagaaccg
     1621 taccgatgag gcaactgaac aggctgttgt tgattacgcc gtcgctttcc cgacacatgg
     1681 tcagcaccgg gccagcaacg agctgcgtaa acaggggggt tttatctccg acagtggtgt
     1741 ccgttctgtc tggttactcc ataaccttga gaatctcaaa agacgatact gaacaaattt
     1801 tatcaggtga cgttccgtaa aaagttgtat ggagttctcg atgcagtcgg cgcgactatc
     1861 agtacccccg ctgaaccctg gactatcggg ccgccgtact ggggcatgtc accctggcgt
     1921 gccgctggtt ttccgctaat ggtttatttc ctctttcttt cccggcaaga atttatttta
     1981 atccggaagg attatttatt ttcagatagg gactcacatt aaacacgtaa cattttaatt
     2041 aatgcatggc tgaatatgta ttacacaatg tcagatatcg taaaagacca gtgcaattct
     2101 atgtaaactc tctgactgtt cattttattt gttgtttcag ggtcggaact caccctcaat
     2161 aaacaactga atacgagtag atattcatcg tctgagctat atggctttac acaatagccg
     2221 acatgtcttt tctcagtcag gaattttact tttcctcact acgaagttga cgacttcgat
     2281 atgggataga ctcttaattc aagcaattac tctgcacaag catcaggcaa tccagctcca
     2341 atatttgtta taaaataatg cgtaacatta atttcaccaa caaaaaaagg tttacaccat
     2401 ccccttgccc tggtatctta ccatatttac accacacttt ttttgtatct ggatcattct
     2461 ttgatacgat tttcgccact tgttccctgg ccctcactgt caatgttttc ttaaacctac
     2521 ccccatcatc atttgttagg gaaaaataag aaaaaaaata atcatcttct acatttgtgt
     2581 atattgatat atccaccgct acgtgcgaaa aaaaatctgg gagccatgta aaaactccat
     2641 cattttctgg tattgccata tagtttatgt aatgtttact tacaagagat tcatctttgt
     2701 ataaataaag ataagtaatt acgcataaaa caacaatgat tataatagca aaaataaata
     2761 ttatcatctt tgatagatta cttgagatag ccagcatctt gtaaagcctt tatcgttttt
     2821 ttatgctctg gattaatata atcactacat ctatctgagc aatctgttgt tgatggacat
     2881 gtcaacccat ggtcatttac agccaagtct tttttattat cttcaatcat ttcagaatgg
     2941 gatagcttta cttttctgcc gtacataccg aacagattta atccgtaatc tctaatctct
     3001 ttttcataac ccagcccttt cgccgatcgg ctaacaccag cgtcatttaa tttagacact
     3061 cgacaaaatg ccatacaatg gaaaaattga tcagtcccac ctaaatttaa tctcttcata
     3121 agtatcagat aatatatccc atgct
//
LOCUS       DADLFA010000270         3099 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000270 DADLFA010000000
VERSION     DADLFA010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.272"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..923)
                     /gene="curA"
                     /locus_tag="JQH65_003916"
     CDS             complement(<1..923)
                     /gene="curA"
                     /locus_tag="JQH65_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HAY6141153.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSHVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGGAGGAEKCRHAIEVLGFDVCLDHHAGDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDK"
     gene            1104..1622
                     /gene="mddA"
                     /locus_tag="JQH65_003917"
     CDS             1104..1622
                     /gene="mddA"
                     /locus_tag="JQH65_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HAY6141154.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDSGKHVMVAGIESQNQASLHLHHSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            1619..2068
                     /locus_tag="JQH65_003918"
     CDS             1619..2068
                     /locus_tag="JQH65_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY6141155.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(2069..2302)
                     /locus_tag="JQH65_003919"
     CDS             complement(2069..2302)
                     /locus_tag="JQH65_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HAY6141156.1"
                     /translation="MSHLDEVIARVDAAIEESVIAYMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(2388..2561)
                     /locus_tag="JQH65_003920"
     CDS             complement(2388..2561)
                     /locus_tag="JQH65_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GhoT/OrtT family toxin"
                     /protein_id="HAY6141157.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            2756..2851
                     /gene="yncL"
                     /locus_tag="JQH65_003921"
     CDS             2756..2851
                     /gene="yncL"
                     /locus_tag="JQH65_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HAY6141158.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
BASE COUNT          755 a          792 c          722 g          830 t
ORIGIN      
        1 attttatcct ctttcaccca ttgccccatc tccttctgaa actcatggat gcggtgacca
       61 taatcctgag cgataataaa cccttgcaag cgaatacgtt ttttcagcac cgttgccatc
      121 aacaggggta aacgatccgg accgggtggt agctctgtag cgttatagct gctcactaag
      181 ccacaaacgg gaatgcgcgc agatgtatta agtaatggta gcactgcatc aaatacctta
      241 ccgcccacgt tttcataata gatatcaata ccttttgggc acgctttcac cagttgttcg
      301 gcaaaatcac ccgcgtggtg atcaagacaa acatcgaagc ccagcacctc gatagcatgg
      361 cggcattttt ccgcgccacc ggcccccccc accaccctgc aacctttaag tttgccgatt
      421 tgccccaccg ttgctcccac aggacctgtc gccgcagcta ccaccaacgt ttcgccctct
      481 ttaggctgac cgatatccag cagccccata taagcggtaa agcctggcat ccccagcaca
      541 cccagcgacc acgatggatt ttgcggatga tcgccaagtt tcaccagatc atcaccactg
      601 gatatgtcat agtcctgcca tccactgtag cccagcaccc agtcgccagg ctgataatca
      661 ggatgattcg actccacaac atggctcacc gtgccgccga ccatcacgcc gccaatatca
      721 acaggcggtg aataagatgg ctcatcgctc atacgtccac gcatatacgg gtccagggac
      781 aaataaactg tgcgcagtaa cacctgtcct tcacccggtg tggcgacatc atcttcttca
      841 agacggaaat tctccggaac cggtgcgcca tgtggacgcg aggccagaac ccaacgtcga
      901 ttacgctgct tttgttgccc cataatgctc tcctgtgctt ttggcatgaa tccatcaata
      961 gtagcaggct cacgggattg gttttgtttg cagccgtcac atcccctctg caacacgcaa
     1021 taatttcatc gaggaatccg ttgcttcggg ctgttttcga tagtattgat aacatcatca
     1081 tcattatttt atcgaggcca gacatgtcca tccgttttgc ccgcaaagcc gattgtgctg
     1141 ccattgcgga aatttataac cacgccgtgt tgtatacggc ggctatctgg aatgaccaaa
     1201 cggttgatgc agataaccgc attgcctggt ttgaagcgcg gaccatagca ggttatccgg
     1261 tgctggtaag tgaagaagat ggcgtagtga cgggatatgc ctcgtttggc gactggcgta
     1321 gtttcgatgg ttttcgccat accgtggaac attcggttta tgtccatccc gatcatcagg
     1381 gcaaaggtct ggggcgtaaa ttgttaagcc gattgattga tgaagcgcgg gatagcggga
     1441 agcatgtcat ggtcgccggg atcgaatcgc aaaatcaggc ctcgctgcat ctgcaccatt
     1501 cgctgggatt tgtcgtcacc gcacaaatgc cgcaggtggg cactaaattt gggcgctggc
     1561 tggatctgac atttatgcag ttgcaactcg acgaacgcac tgaaccggac gcgattggat
     1621 gaatcagtcg ctcacccttg cttttttgat tgccgccgga attggtctgg tagtccagaa
     1681 cacgctgatg gtgcgcatca cccagacctc ctctaccatt ctcatcgcca tgttgctgaa
     1741 ctcactggtg gggattgtgc tctttgtttc tattttatgg tttaaacaag gcatggcggg
     1801 gtttggcgaa ctggtgtcca gcgtgcgctg gtggacactt atacccggct tgctgggatc
     1861 gttttttgtc ttcgccagta tcagcggata tcagaatgtg ggagcggcga caactattgc
     1921 agtactggtc gccagccagc taattggcgg tctgatgctg gatatcttcc gcagtcatgg
     1981 cgtaccacta cgggcgttgt tcggcccaat ttgtggcgca attttacttg tggttggcgc
     2041 ctggttggta gccagacgct cattttaatc agaggatggt gccgcctttg gttaactgtt
     2101 cgtggcgcgc ttccatatct tctttatgct tgcgaccgtg atgagcaata gcggtgcgca
     2161 aacgctgctg ctgggtgtag cgatcttccc gacttaactc cgcgtcatcg ctcagggcga
     2221 ttaataattc gttcatatag gcaatgacgc tctcttcaat cgcggcatcc acgcgcgcga
     2281 tgacttcatc cagatgcgac atactttctc cttaaccact tgtgaaattt atcgattcat
     2341 ccaaatgata taagtctgcg tggtgccgtc aagccagtga gtaagattta aaacagtgaa
     2401 aacaacagcg ccaccgggaa actcatcggc catgttgccc ccaccagaaa ggcgcttaag
     2461 aaacggatgc gtttcttatc gtgagaaaga aaccaggtga taagaaatgc gattgctgcc
     2521 ataaccgcat aaaaaacaag catatgttga tagagagaca tgtacgggcg tccagccaga
     2581 tgaataaaac gcgcgcaaat atgcgtcatt ttttgacgtg catcaagttt tttcaccagt
     2641 ttgagaaaaa atcatcaaac agcaacttgc ccttatccat cttcagaatg agtattattg
     2701 cggattttct taacccgtac tatacacata ggggcaatga taaaaggtgg caaaaatgaa
     2761 tgtttccagt agaactgtag tactgataaa tgtctttgct gctgttggtt tgtttactct
     2821 tatctctatg agatttggct ggtttatttg atgtagtgat tttgcacgca gcacgtactg
     2881 tcagagaaat caactctctg acagtacgca agatcttctc atactctcca ggtttacaac
     2941 attatcctta atatttactt ctttttgctg gcatctacat tgaaatgagt agaaaacatc
     3001 cccctactgt cgtctataca aacagcttac tgattcaaac ggaatcttgt tttcacattt
     3061 ttctatcatg aatactttat acgcgcggga gtctataaa
//
LOCUS       DADLFA010000271         3086 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000271 DADLFA010000000
VERSION     DADLFA010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.273"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..191)
                     /locus_tag="JQH65_003922"
     CDS             complement(<1..191)
                     /locus_tag="JQH65_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HAY6141159.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQR"
     gene            384..886
                     /locus_tag="JQH65_003923"
                     /pseudo
     CDS             384..886
                     /locus_tag="JQH65_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(932..1348)
                     /gene="rraB"
                     /locus_tag="JQH65_003924"
     CDS             complement(932..1348)
                     /gene="rraB"
                     /locus_tag="JQH65_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HAY6141160.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1510..2514
                     /gene="argF"
                     /locus_tag="JQH65_003925"
     CDS             1510..2514
                     /gene="argF"
                     /locus_tag="JQH65_003925"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAY6141161.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIAETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            complement(2586..>3086)
                     /locus_tag="JQH65_003926"
     CDS             complement(2586..>3086)
                     /locus_tag="JQH65_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY6141162.1"
                     /translation="SRKLQELIWDKLVAYVNDFNLSNQEKSRLILRLFDDVKLLFNEV
                     PVSILVNDIFLKDFFMKQPDFAKWYFYQLLKKYEGEQLYLNELGYVYGNEEKTNEIVN
                     NNPGYVVEIFEEKMGNELKIRTRMMEILRDGKINICEYINKEQLEKLNPPEDLRIAIK
                     KLGWNN"
BASE COUNT          863 a          701 c          659 g          863 t
ORIGIN      
        1 cgttgtccgt taacttccat attagcatag agataacgct tacatttcat taatgcccat
       61 ggtaaataga tacctagcgt tataatcgtt aacaacacat tcaccacaca aataagaaaa
      121 tatctctcac ctgtaccatg aaaaacaaac gaatgggacg gaacatccat ttcattaata
      181 acttgagcca taaaaattcc ctgaattaaa aaatccattg atataattta tatatacaga
      241 cagtaaaatg ttctgccacg ttacaatact cttatcagta ataggctggc aaaaacttta
      301 ttgctttata agatgcttgc gcacgtaaga agagagtggt attaaacaaa tgtataatta
      361 ctttattttt attgggttaa actatgaata acattgcgcc gcaatcacct gtaatgcgtc
      421 gcctgacgct acaggataat cctgctattg cccgcgtcat tcgtcaggta tccgccgaat
      481 acggtcttac cgctgataaa ggctacaccg tcgccgatcc gaatcttgac gaactgtatc
      541 aagtgtatag tcagccgggt catgcatatt gggtggttga gtacgagggt gaagtggtcg
      601 gcggcggtgg gattgcgcca ttagcaggta gtgaatcgga catttgcgaa ctgcaaaaga
      661 tgtattttct tccggctatt cgcggtaaag ggctggcgaa aaaactggcc ttaatggtga
      721 tggagcaggc acgagagatg ggcttcaaac gctgctatct ggaaacgacc gctttttaaa
      781 ggaagccatt gcgctttatg agcatttggg ctttgagcat atcgactatg cacttggctg
      841 cacgggccat gttgattgtg aagtgcggat gttgcgcgaa ctctaaaaaa atgcccgcta
      901 gtgtgtgcta tcgggcattt taatcaatta attagtggcg aaccccgtca tcgtcttcat
      961 cgacaaaatc ttcatcgtcg ccgtcttcgc cattaggatc ttcaaaataa gtgccccaac
     1021 cgtcgtactc aacgtcaaat ttctccgcca atgtcatcag ttgttcaacc tgggcatcga
     1081 tcagatcggc attcaacgcg cactcgctga ggatgtcgca gcaaatcacg atatcaccat
     1141 cttcaacttc cagctcttct ggatcggtca cttcgtaacc gagtttaaac gcttcaactg
     1201 ctgctttttc cagggtttcc agatcgtctg cggaaagatg gtgttcgatg gtgtacagcg
     1261 cgtccggatc gctgccatct tccagtaatt cttcaataat caaacgagtt tcttcacgct
     1321 gttcttccag ttgttccggg tttgccatgg ctcattcctc ttaatgtcct gccgatactc
     1381 ttattgtcac ataccactga cattgcctcc acctttgtga caaagattta tgctttagac
     1441 ttgcaaatga ataatcatcc atataaattg aattttaatt cattgaggcg ttagccacag
     1501 gagggatcta tgtccgggtt ttatcataag catttcctga aattactcga tttcacgcca
     1561 gctgaactca acagcctgct gcagttagcc gcgaagctga aagccgataa gaaaagcggc
     1621 aaagaagaag ccaaactcac tggtaaaaac atcgcgctca tcttcgaaaa agactcgacc
     1681 cgtacccgat gctctttcga agttgccgca tatgaccagg gtgctcgcgt tacttatctc
     1741 ggcccaagcg gcagtcagat tggtcataaa gagtcgatta aagacactgc ccgcgtgctg
     1801 ggtcgcatgt atgacggtat tcagtatcgc ggctatggtc aggagattgc cgaaacactg
     1861 gcggaatacg ctggcgtgcc ggtatggaac ggcctgacca atgagtttca ccccacgcag
     1921 ttgctggcgg atctcctcac catgcaggag catttacccg gtaaagcatt caacgaaatg
     1981 acgctggtct atgcaggtga cgcacgtaac aacatgggca attcgatgct cgaagctgcg
     2041 gcgcttaccg gtctggattt gcgtctggtc gcgccacagg catgctggcc ggaagctgcg
     2101 ctggttacgg aatgccgcgc cctggcacag caaaatggcg ggaatattac gctgacggaa
     2161 gatgtagcca aaggagttga aggtgctgac tttatctata ccgatgtatg ggtgtcgatg
     2221 ggggaagcaa aagagaaatg ggcagaacgg attgcattac tgcgtgatta tcaggtgaac
     2281 agcaagatga tgcagttgac cggtaacccg gaggtcaaat tcctccactg cctgcccgcg
     2341 tttcatgacg accagacgac gcttggtaag aaaatggcgg aagagtttgg cctacatggc
     2401 ggaatggaag tgactgatga ggtcttcgaa tctgccgcca gcattgtttt tgatcaggcg
     2461 gaaaaccgca tgcataccat caaagcggtg atggtcgcga cgctcagtaa ataattgata
     2521 tcgcaataaa gatgccggga aatacaaaaa catcccggca tcacattatt ttatgcagta
     2581 atagttcaat tattccaccc caattttttt attgctatac gtaaatcttc cggaggattt
     2641 aatttttcta actgttcttt attaatatat tcacatatat ttattttccc atcccggagg
     2701 atttccatca ttcttgttct aatttttaat tcattaccca tcttttcttc aaaaatctca
     2761 acgacataac ccggattgtt atttacaatt tcatttgttt tttcttcatt accataaaca
     2821 tatcctaact cattaagata cagctgttcg ccctcatact tcttaagtaa ttgatagaag
     2881 taccacttcg caaaatcagg ttgcttcatg aaaaaatcct tcaaaaaaat atcatttact
     2941 aaaatgctga caggcacttc attaaatagc aacttaacat catcaaataa ccttagtatt
     3001 aagcgtgatt tttcttgatt gcttaagtta aaatcattga catatgcaac caacttatcc
     3061 caaatcaatt cctgtaattt acggct
//
LOCUS       DADLFA010000272         3067 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000272 DADLFA010000000
VERSION     DADLFA010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.274"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(51..235)
                     /locus_tag="JQH65_003927"
                     /pseudo
     CDS             complement(51..235)
                     /locus_tag="JQH65_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311258.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
     gene            complement(397..471)
                     /locus_tag="JQH65_003928"
     tRNA            complement(397..471)
                     /locus_tag="JQH65_003928"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(436..438),aa:Arg,seq:cct)
     gene            complement(547..1479)
                     /locus_tag="JQH65_003929"
     CDS             complement(547..1479)
                     /locus_tag="JQH65_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HAY6141163.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETHDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            1773..2528
                     /gene="mlaA"
                     /locus_tag="JQH65_003930"
     CDS             1773..2528
                     /gene="mlaA"
                     /locus_tag="JQH65_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HAY6141164.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(2710..3045)
                     /locus_tag="JQH65_003931"
     CDS             complement(2710..3045)
                     /locus_tag="JQH65_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141165.1"
                     /translation="MAESFATGSDHQVVNELNGERLREPNEVFKRLGRAIRYNFQVDD
                     AKFRRDNVKEIISTLFANKVDVDHPENKYKDFKDLEDKVEKRLQNRQTEYQNEINQLS
                     APGVNFDDI"
BASE COUNT          766 a          707 c          712 g          882 t
ORIGIN      
        1 cccatctttt acggggtgaa ggtagatttc gtatgaaggg tatctgttgt ctcctgcaaa
       61 gtcataagcg gatattttcc tacggtcagt atttttttct taccgtcaat cttgtagcga
      121 agttgccata ccttttcccg gatacaggaa cataaaggta caggccatta ccatcgagaa
      181 ggcggtatgg tttttctttc ggctttgctg cttcaatctg cttaacggtg agcatgggta
      241 aaaatccggt gggtaaaatt attttatcca ctttttaccc gtcatggagt gcggctgtca
      301 acgatctgac gcgaaccatg acgaaccgtg aatatacgga aggcttgata ttcaggggat
      361 tttgcggact ggtacggatg ggagcgaact gataaatggt gtcccctgca ggaatcgaac
      421 ctgcaattag cccttaggag gggctcgtta tatccattta actaagagga caatgcggca
      481 tgagtatacc cgctaatgga ctgcggagta agtacactgc cgctcgattg cttaaaccct
      541 cgccatttat gctgggtttt tatcattttt cttaatgttt tccgcacgtt ctgctttttg
      601 gcgtgcttct gctttgcgct tgttgctcat gtcgttacgg atctgtgcat gactcattaa
      661 cgcgaagata aaggtgccgc cgcagatatt ccccgctaaa gtaggtagtg cgaagggcca
      721 aatgaaatcg ctccagtgca gcgtgccgtt aaacaccaga tagaggattt ctacagaacc
      781 gacaacgatg tgggtggtgt cacccagtgc aataagccag gtcatcaata taatcaccac
      841 aatctttgcc gcacccgctg caggaaacat ccaaaccata gtggcgatca gccagccgga
      901 aatgatcgcg ttggcaaaca tctcgctggg ggtgttcttc atcacatcca tgccgatttt
      961 gacaaatgca tcgtgagttt cttcattgaa gataggcata tattcaaatg cccatgcagc
     1021 aatacctgtc ccgagaatat tacccagcag cacgacgccc cataatcgca taagtaagcc
     1081 gacgttgctc attgtcggtt tttgcatgac gggtagtacc gcagtcacgg tgttttcggt
     1141 aaataattgc tggcgggcca taatgacgat aataaaacca aaggtataac cgagattctc
     1201 caacaagaag ctgcctggca cgccttccag ttcgacatga aatatccctt ttgccagtaa
     1261 cgaagcgccc atcgacagac ccgccgcaat ggctgaccac agtagtgcca ttgcgtcgcg
     1321 ttccagctct ttttcaccat cctggcggat atgttcatga attgccatcg cccgggaggg
     1381 gagtcgatct tcatctattt ctattttttt gccgcgctct ttttcttcgc tctcaacttc
     1441 aatttcgtcg ctgtgttgat caattttgtc gttgtccatg gtctcttctc ttgaattagc
     1501 acgtatagct aaagcgtagc ggcttttttg ctcgcaactg gcgggagtta ctctgaaaat
     1561 gtagaaaagg ccgcgtttgc ctttttctgt ttctatagaa tcaagtagcc tacagggcgg
     1621 cgattaccag gctatgatca aatcagcaaa tcagggcgtc tggacatcag ttgacgtgct
     1681 gttacaatcg cccacaccta aacaggcgga tacggtatcg ttccgtcatg gatggcaaac
     1741 tgcataagcc ataaaaaaac agggagacat ttatgaagct tcgcctgtcg gcgcttgctc
     1801 tgggaactac gcttctggtg gggtgtgcga gttccggtac agatcagcaa gggcgttctg
     1861 acccgttaga agggttcaac cgcaccatgt acaacttcaa cttcaatgta ttagacccgt
     1921 atattgttcg accggtcgct gtcgcctggc gtgattatgt tccgcaaccg gcgcgtaacg
     1981 gtttgagcaa ctttaccggc aaccttgaag aacctgcggt gatggttaac tacttcttgc
     2041 agggcgaccc ttatcagggg atggtccact ttacccgctt tttcctgaac accattttgg
     2101 ggatgggcgg ctttattgat gttgcaggga tggcgaaccc gaaactgcaa cggactgaac
     2161 ctcaccgctt cggtagtacg cttggtcatt atggcgtggg ttatgggcct tacgttcagt
     2221 taccgttcta cggtagcttc acgctgcgtg atgacggtgg tgatatggcg gatggtcttt
     2281 acccggttct ttcctggctg acctggccga tgtctgtggg taaatggacg cttgaaggga
     2341 tcgaaacccg cgctcagctg ctggattccg atggtctgct gcgtcagtcg tccgatcctt
     2401 atattatggt gcgcgaagcg tacttccagc gtcatgattt catcgctaat ggcggcgaac
     2461 tcaaaccgca ggaaaatccg aacgcacaag cgattcagga tgatttaaaa gatattgatt
     2521 ctgaataaga aacaaataaa aaaggtgagt cgcaaaactc accttttttg ttgttatccc
     2581 tcaattacca caagtaaaag gagataaacc gacaatgacc cggacgtaat gatgtaaaaa
     2641 tactgatgtt cgtcactgac tatttccgat attcaggcgt tgaaaaatca aaaaactgga
     2701 aaaataattt taaatatcat caaaattaac acctggcgca gatagttgat taatttcatt
     2761 ttgatattcc gtctggcgat tttgaagcct tttttcaact ttatcttcga gatccttaaa
     2821 gtctttgtat ttattttcag gatggtcaac gtcaactttg ttagcgaata aagtagaaat
     2881 tatttctttt acattatcgc gtctaaattt tgcatcgtcc acttgaaaat tatatcttat
     2941 tgctcgacct aaacgtttaa aaacttcgtt aggttctctc agtctttccc cgttgagctc
     3001 gtttacaacc tgatgatcgg aacctgttgc aaatgattct gccatgatag ctttactgct
     3061 ttcaaac
//
LOCUS       DADLFA010000273         3020 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000273 DADLFA010000000
VERSION     DADLFA010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.275"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(21..323)
                     /locus_tag="JQH65_003932"
     CDS             complement(21..323)
                     /locus_tag="JQH65_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141166.1"
                     /translation="MLEIVLSPVKAQQFTVTLGAQVCTIRLNQRTTGMYIDIIVNGEP
                     CLYGVLCLNNNRIVRYGYLPFQGDLFFTDTEGNSDPDWRGLGSRYRLYWLLPEELI"
     gene            complement(323..1033)
                     /locus_tag="JQH65_003933"
     CDS             complement(323..1033)
                     /locus_tag="JQH65_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000010340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141167.1"
                     /translation="MSFSFDNISLNNFSLSESNVLSAVRGGGVLGLINSVLAPSFGIY
                     YARNDPAGAHQKGGKPFSPDSFVVVEVGAEASVSTVPVEQGAYTTFNKIQRSPELHVT
                     FIVEGWTAFSGSVPNLTNFSTTSRTNVLETLERMRTTAGLYDIETPDKTWTSCDLVKY
                     DYRMRSNNGPTLLTVTAVFQTVMNTGEVSVGSTDKSSPTDNDKAKGAASVKTQPVTAS
                     VTQPSDADRRSATNRGIT"
     gene            complement(1030..2007)
                     /locus_tag="JQH65_003934"
     CDS             complement(1030..2007)
                     /locus_tag="JQH65_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005117211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HAY6141168.1"
                     /translation="MTKALSGPGGLLFAITALYPVVDGLLTSIVGRENKDWLDSHGFF
                     WASDGTFFFNKKEMETYQTKLDVGEKPGNITRAQSPTVWQQGMLDTQASQATGREAAF
                     GESWLQGMRATQEKLGNAMQNRQRPTKTGEALLGWLQPKLSQLEEKYNLPPGLLRSVA
                     ITESRGNQFAVSPAGAMGLFQFMPKTASEFGLRGNDAFDPEKAADAAARKLGGLIRFF
                     HGDLAKALAAYNWGEGNVQRKGLAAAPEETRNYIPRVLANLPYPGAAMAIRSLHPAPV
                     SQSTVTETTHIGTLNVTTTSDNVKGITDDARRRITNSALVSVYSSGVTG"
BASE COUNT          690 a          890 c          725 g          715 t
ORIGIN      
        1 tattttacgt tgtctgtagc tcatatcagt tcctcaggta acagccagta gagccggtac
       61 cgggatccga gcccccgcca gtcagggtca ctattcccct ccgtgtcggt aaaaaacagg
      121 tccccctgaa acggcaggta tccgtaccgg acaatccggt tattattcag acacagcacg
      181 ccatacaggc acggttcacc attaacaatg atatcgatat acatccccgt agtacgctga
      241 ttcaggcgaa tggtgcagac ctgagcgccc agtgtcaccg taaactgctg ggctttaacg
      301 ggagataaaa caatttccag catcaggtga tccccctgtt cgtggcactt cgtctgtcag
      361 cgtctgacgg ttgtgtcacc gacgccgtaa ctggctgcgt tttaaccgat gcggcccctt
      421 ttgctttatc gttgtccgtg ggagacgact tatccgtact tcccactgac acctctcctg
      481 tattcattac ggtctgaaat accgcagtaa ccgtcagtaa ggtcggacca ttattacttc
      541 gcattcggta gtcatatttc acaaggtcgc aggatgtcca tgtcttgtcc ggcgtctcaa
      601 tatcgtagag tcctgccgtg gtacgcatcc tttcaagcgt ttccagcaca ttcgtccgcg
      661 aggtggtgga aaaatttgtc aggttcggga cggatccgga aaaggccgtc cacccctcta
      721 caatgaaagt cacatgcagt tccggcgatc tctggatttt attaaaggtg gtataagccc
      781 cctgctccac ggggacggtg gaaacagaag cctccgcgcc cacctcaacg acaacaaaag
      841 aatccgggga gaaaggtttg ccacccttct gatgggcacc tgccggatca ttccgtgcgt
      901 aataaatacc gaatgacggt gccagtacac tattaatgag tcccaggaca ccgccgccac
      961 gaactgcact cagtacgtta ctttcactga gcgaaaagtt attcagggaa atattatcga
     1021 aagaaaaact catcctgtta ccccgctgga ataaaccgac acaagcgccg aattcgtgat
     1081 acgccgacgt gcgtcatcgg taataccctt cacattgtcc gacgttgtgg tgacattcag
     1141 tgtcccgata tgcgtggttt ccgttacggt ggactgcgat acaggcgcag gatggagcga
     1201 ccgtatggcc attgccgcac ccggataagg caggttcgcc agcacccgcg gaatatagtt
     1261 gcgggtctcc tccggagcag cagccagtcc cttacgctga acatttccct ctccccagtt
     1321 atatgccgcc agagccttag ccagatcgcc atgaaaaaaa cgtatcaggc caccaagttt
     1381 tctcgcggcg gcatcagcgg ctttttcggg atcaaaggca tcgttccccc tcagaccaaa
     1441 ttcactggcc gtcttcggca tgaactgaaa cagtcccatc gcaccagcag gtgagacggc
     1501 aaactgatta ccacgggatt cggtgatcgc aacgctgcgc agcagtccgg gcggcaggtt
     1561 atatttttcc tccagttgag acagtttcgg ttgcagccag cctaacaggg cttccccggt
     1621 cttcgtcggt cgctggcggt tttgcatggc attaccgagt ttttcctgcg tcgcacgcat
     1681 accctgtagc caggattcac cgaaggctgc ttccctcccg gttgcctgag aagcctgagt
     1741 atccagcatg ccctgctgcc atactgtggg tgattgtgcc cgggtgatgt tgccaggctt
     1801 ttctccgaca tccagttttg tctgatacgt ctccatctct ttcttattga aaaagaaagt
     1861 cccgtctgaa gcccagaaaa aaccatgtga atccagccag tccttatttt ccctgccaac
     1921 aatggatgtc agtaatccgt caacaaccgg gtaaagcgcc gttatcgcaa aaagaaggcc
     1981 tccggggcca ctgagggctt ttgtcagccc ccgcacccat gacgccactt tcagcccgat
     2041 cagcgtaata atgacattct gccagccccc cagttctccg gcagccttat tcgccaggga
     2101 ggccactgac tcaacgtcat tcagaaatgt ggtgataaac ctgttcactt cctccggatg
     2161 ttgctgcatc cagtcaccga gtgtctccag ccagtggctg agcttcagcg cgtacggcat
     2221 caacgcggtg ctaatcgtca tcccgatgcc ggaccaggtc tgatcgagct caacaagctc
     2281 ttttcgtagc ttacgggcca gcgctgtacg ccccggcgta acctgcgaac gggaagtaaa
     2341 ttcgtccaca tctttcagcg catgaccgga gccaagaaac tgctgtccgg cataactgaa
     2401 gcccagcgcg ttgccgtagg cagtttgttc ggactttgtc agtcgcggaa aaataaccgc
     2461 cagcttgcgc atgatggctt cagtgctgtc agtatttaaa tcaacactaa tgcccgccct
     2521 ggctgcaacc tgaaacaaat cctgtaacac aggatcaaag gaccttccgg ctctgaaggc
     2581 cgcttttgca tttgcaagcc gggaaaatgc cccagacatt tccccttcat caacattatt
     2641 tgcccgcgca gcccgtaacc atccgtccag atttctggcc ttcatgccaa aagcatcgga
     2701 ttccacggac agacggttca gatcacgggc aaaacctgtt accaggttct tcagcccccc
     2761 cagcgtcaga ctgacacccg caagagccag aatctcgcta cggatccctg aaaaaaaacg
     2821 ggacgcccgt ttcccggcat cttccattcg acgggccgtt ttatcagcat cttttccggt
     2881 tttttccagt gattcactgg ttttcttctg tccctgactg aatgtgtcag cgacaccttc
     2941 catcaccgtc gtgagccggt taagggcagc atgtactgcc tgttccccgg cagtaaagtc
     3001 cctgtcatca atcccgaggg
//
LOCUS       DADLFA010000274         2942 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000274 DADLFA010000000
VERSION     DADLFA010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.276"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            577..1293
                     /locus_tag="JQH65_003935"
     CDS             577..1293
                     /locus_tag="JQH65_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141169.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSCIISSLVISIIIRIYGRIFSVSCFSDIPFVREHRIKLIM
                     AGFLVYFWLFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            1845..2249
                     /locus_tag="JQH65_003936"
     CDS             1845..2249
                     /locus_tag="JQH65_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141170.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            2312..2611
                     /locus_tag="JQH65_003937"
     CDS             2312..2611
                     /locus_tag="JQH65_003937"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY6141171.1"
                     /translation="MTKTVSTSKKTRKQHSPEFCSEALKLAERIGVAAAARELSLYES
                     QLYAWRSKLQQQMTSSERESELAAENARLKRQLAVQAEELAILQKAATYFAKRLK"
     gene            2608..>2942
                     /locus_tag="JQH65_003938"
     CDS             2608..>2942
                     /locus_tag="JQH65_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY6141172.1"
                     /translation="MKYVFIEKHQAEFSIKAMCRALRVARSGWYTWCQRRTGISPRQQ
                     FRQHCDSVVLAAAFTRSKQRYGAPRLTDELRAQGYHFNVKTVAASLRRQGLRAKASRK
                     FSPVSYRAHG"
BASE COUNT          824 a          605 c          667 g          846 t
ORIGIN      
        1 ttgtgggcca taaatattcc aaaaaaatag cccgctccgt ttattaccgg aacgggcgag
       61 agcatgacat tgtctgtaat tataatgaga ctgctgattc tacttatttt taatttcaag
      121 aaaagcgttt tctacaaaga attatgagat ttaatgtttt gtttagacaa tattgtcatt
      181 ctgttcaata atgaaagtct tcatcaacaa caagtaaaaa caacgtccct ctcatcgaac
      241 cggacttgca gatttcccgc atccggctct cttgcaacac atcagacctt cacgttcggg
      301 cggttatggg gaaggtccag caagctgtat gagccctaac tgtttgaata ccagtagatt
      361 accagaaact tctatcgatt gaagacatta ccaactagcg ttactaaatg ttgaccaagt
      421 tttgttcact tgtctggttt tgatagtagc attctgcaga gtattttgat atgataaata
      481 tgcattaaca atgatattat tgttaatttc ctagcatgaa aattaaaaga tcaacttttt
      541 atctcgaata ttttttacat tatcagttgg tttcttatga atgacaattc attgcttaga
      601 aattcttcat tattcatcgc ctatatggga tgtgtagggt gggtcagtgc ttattcttat
      661 ggctggggaa cctcttttta ctatggattt ccatggtggg ttgttggtgc aggtttggat
      721 gatgttgcca gaagtttact ctatgctatc atagtaatgg gtatactttt taccggttgg
      781 gggattggta ttttattttt tcttcttata aagaaaagaa gtaaaataca agacttgagt
      841 ttttttagac tattttttgc tattacccta ctgtttttcc ctgtaatatt cgaactttta
      901 attctcaaac aatatttcat acttccatta tcactttcat gcattattag ttcattggtt
      961 atttcaataa taattcgcat atatggacgg atcttttctg tatcatgttt ttcagatatc
     1021 ccttttgtta gagaacaccg cattaagtta attatggctg gttttttggt ttatttttgg
     1081 cttttttcct ttcttgttgg ctggtataaa cctcaattaa aaaaagaata tcagatgctt
     1141 tgttataata atagttggta ttatgttctt gctcgttatg attcacggct cgttttgtct
     1201 agttcattta aagatgacag taacagattc ttaatattta atacagaaca gtcgggattt
     1261 tatgaaatca atgatgttta tgtcagaaag taataatcct ctcccacaaa gtgaattaaa
     1321 cgaatatata atgcgttcga agtatatgta ttgagctgtc atactgagaa acaaacctta
     1381 gggcagcaca attaaagcca gaaacaacta tgcaagaaag ttactaaata agtgctcgga
     1441 acagtaaaac actcaggggg gactcagtcc agattatgta atcagataaa ttgccaaaca
     1501 tcacaaatca ccagcaggac tgagtgatgc cgagcattgc accaataccc cgtgatgaac
     1561 gatgcctgat gcagaaagct ccctataaaa caaagaacaa aaactacgac cggcgattca
     1621 ccgtcatact gatgctgcat cgtggtgaca ctgtctgtca ctttcatgtt gactgaacaa
     1681 ctctgctgcg aagaaggtgt accgccggcg ggatttgatt gtccggcgtc tagcttccac
     1741 ttccctgatg gaactgaaag ttgttatggc cggtcagctt gccctgtccg caggacatga
     1801 ggtgctgtga cggacagcac cagtaacgga aagtgacaat aaaaatgaat actgcgctga
     1861 tagttgcact aatgtgtatg tggtatgcgg tacccgctgc tgcaaaagag actttactgg
     1921 ctatgccacg taattcaaca gaacattgct atgcggagat taatgtgcac ggaccatatg
     1981 gggtgtattt ccgagtggtc ccgcatcccc ccgggggaaa gagctgggtc gaatgtaaca
     2041 gtgactacta ttactctgat aaaccaccgg gagtacagat actcggaacc cgtgcagggt
     2101 gtcgggtata cggaatctgc gggaccacct cgacactgca tgtggctgga agaggggttg
     2161 tgtgtattaa aaatatatgc agtccacgtg ggatgataat tcacaggata cggaaacgtc
     2221 ctgtagtggc tgtaagcgat gagatgtgat cttatccagc aatagtggac acgcggctaa
     2281 gtgagtaaac tctcagtcag aggtgactca catgacaaaa acagtatcaa ccagcaaaaa
     2341 aacacgtaaa cagcattcgc ctgaattttg cagtgaagcc ctgaagcttg ctgaacgcat
     2401 cggtgttgct gccgcagccc gtgaactcag cctgtatgaa tctcagctct atgcgtggcg
     2461 cagcaaactg cagcaacaga tgacctcgtc tgagcgtgaa agtgaactgg ccgctgaaaa
     2521 tgcccgcctg aagcggcaac tggcggtgca ggcagaggaa ctggctatcc tccaaaaggc
     2581 cgcgacatac tttgcgaagc gcctgaaatg aagtatgtct ttattgaaaa acatcaggct
     2641 gagttcagca tcaaggcaat gtgccgcgcg ctccgggtgg cccgcagcgg ctggtatacg
     2701 tggtgtcagc ggcggacagg gataagccct cgtcagcagt tccgtcagca ctgcgacagc
     2761 gttgtcctcg cggcggcctt tacccggtca aaacagcgtt acggtgcacc acgcctgacg
     2821 gatgaactgc gtgctcaggg ttaccacttt aacgtaaaaa ccgtggcggc aagcctgcgc
     2881 cgtcaggggc tgagggcaaa ggcctcccgg aagttcagcc cggtcagtta ccgcgcacat
     2941 gg
//
LOCUS       DADLFA010000275         2918 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000275 DADLFA010000000
VERSION     DADLFA010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.277"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..1322
                     /locus_tag="JQH65_003939"
     CDS             <1..1322
                     /locus_tag="JQH65_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY6141173.1"
                     /translation="LVVVFSWVFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTM
                     GPGLAYLAAWTYWVVHIPYLAQKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLF
                     IFFMWVASRGMKSLKIVGSVAGIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFI
                     PHIDFTYITTISMLVFAVGGAEKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSL
                     AMGMMFDSRNIPDDLMTNGQYYAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSID
                     APLKVLLGDADSKYIPASLCRTNASGTPVNGYFLTLVQVAILIMLPTLGIGDMNNLYK
                     WLLNLNSVVMPLRYLWVFVAFIAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFA
                     CLTGIFPKMEAFTAEWTFQLALNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(1323..2231)
                     /locus_tag="JQH65_003940"
     CDS             complement(1323..2231)
                     /locus_tag="JQH65_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY6141174.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINDARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLGDWSTERPGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQISQSQRHRPQ"
     gene            2331..>2918
                     /locus_tag="JQH65_003941"
     CDS             2331..>2918
                     /locus_tag="JQH65_003941"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HAY6141175.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTPEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPLWWYSFPAMLKGYI
                     DRVWNNGLAYGDGHKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGEELHASYYQSLLSQVRDMVDAL"
BASE COUNT          738 a          622 c          728 g          830 t
ORIGIN      
        1 ggctggtggt tgttttttca tgggtgttta tctttgcgct ctatttcaca ccttatgcgc
       61 taattgttgg tcagttaggc tcgaccttca aagatgggaa gggcggggtc agtacctgga
      121 ttaaacacac gatgggaccc ggactggctt atctcgccgc gtggacctac tgggtggtgc
      181 atattcccta tctggcacaa aaaccccagg caattctgat tgcgctcggt tgggcgatga
      241 aaggcgacgg ttcgctaatc aaagaatatt cagtcgtagc gttacagggg ttaacgctgg
      301 tgctgtttat cttctttatg tgggttgctt cacgcggtat gaaatcgctg aaaatcgtcg
      361 gttctgtggc agggattgcg atgtttgtta tgtcgctcct gtatgtggcg atggcggtaa
      421 ccgcgcctgc gattactgaa gtgcatattg cgaccacaaa cattacctgg gaaacgttca
      481 ttcctcatat cgactttacc tacattacca ctatttcaat gctggttttc gcggttggcg
      541 gagcagagaa gatttctcct tacgttaatc aaacgcgcaa cccaggaaaa gaatttccaa
      601 aagggatgtt atgcctggcg gtgatggttg cggtttgtgc cattctgggc tcactggcga
      661 tggggatgat gtttgactcg cgtaatatcc cggatgactt aatgactaac ggtcaatatt
      721 acgcctttca gaagctgggc gagtattaca acatgggtaa tactttaatg gtgatttacg
      781 ccattgcgaa taccctggga caagtagctg cgctggtatt ctcgattgat gccccgctta
      841 aagtgctatt aggtgatgct gacagcaaat atattccagc cagtttatgt cgtaccaacg
      901 cttctggtac gcccgttaat ggctattttc tgaccctggt gcaggtggca attctgatta
      961 tgctgccgac tctcggcatc ggtgatatga acaatctcta taaatggctg ttgaatctta
     1021 actcggtagt gatgccgctg cgttatttat gggtatttgt tgcatttatt gcagtcgttc
     1081 gcctggcgca gaaatataaa ccagaatatg tctttattcg taataagcct ctggcaatga
     1141 ccgtcgggat ttggtgtttt gcctttaccg cctttgcctg tttgacgggg atcttcccga
     1201 aaatggaagc cttcactgca gagtggacct tccagttggc gctgaatgtt gcaacgccgt
     1261 ttgtgctggt aggattagga ctgattttcc cgctgctggc gcgtaaagcg aatagtaaat
     1321 aattattgtg gtcggtgacg ctgactctgg ctgatctgac ccgtttttac ataatcgaga
     1381 aatgcgcgca agccgcagga catatactgg cggtttggat aatagatctg gaagcctgga
     1441 cgctctgtgc tccagtctcc cagtacacat tctaaacgac cagtatcaag gtactccttg
     1501 atctgttcat agagcagata cccgatacca gcacccatca aaacggcctc cagttcagca
     1561 tcaacatcat ccagaatgat atttcctgcg acagcaattt ccagctcctt tgcaaattgc
     1621 cagtggaaag gtttgccgct gggataacga aaaacaacac attgatgatt aagcagatca
     1681 tgaggatggc ggggtttgcc atatcttgca aagtactctg gcgttgctgc tacacataat
     1741 ttcactggcg ggccgatcgc cacgctgatc atatcttttt cgacgatgcc actaagtcga
     1801 acgccagcgt caaagccctg ttggacgata tcgacaagag agtcgtcagt tgtgagctcg
     1861 actttaatat caggatattc acgtgtgaaa ccaaccagta aaggcattaa aaatatacgt
     1921 gcagctaccc gggcgtcatt gattttgagc gttcctgtcg gtgtcagacg aaaatcattc
     1981 atctcatcta acatgatctg aatttcgtcg aaagctgggc gcaatcgttc atagagatta
     2041 gaacccgctt ccgttaacga tacgcttcgg gttgtccgat tgaataagcg aattttaaga
     2101 cgttgttcca gtgttttaat actatggcta atggcggacg aggataagcc taactcatcg
     2161 cccgctgcac gaaagctttg attacgggcc acggcaaaga aagtggcaaa gtcagacata
     2221 ttcatccgca ttagtgaaaa tccttcatta actcatcctg aatcatcacg tttacaggat
     2281 tatgccgcct gcgtaaagtg cgctccagaa cttaacgtgg aggtaaaatt atgcagtctg
     2341 aacgtattta tttggtatgg gcccatcctc gtcatgattc attgaccgca catattgctg
     2401 atgcgatcca tcagcgggca atggagcgga aaatacaggt gacggaactc gatttatatc
     2461 ggcgtaattt caacccagtg atgacgccgg aagatgaacc agactggaag aatatggata
     2521 aacgttattc tccagaggtt catcagcttt attcagagct gcttgaacat gacacgttag
     2581 tggtggtttt tcctctctgg tggtacagct tcccggcaat gctaaaagga tatattgaca
     2641 gagtatggaa taatgggctg gcttatggag atgggcacaa attaccattc aataaagttc
     2701 gttgggtggc gctggttgga ggagacaaag aatcatttgt ccagatgggc tgggaaaaaa
     2761 atataagcga ttatttgaaa aatatgtgca gttatcttgg tattgaagat gccgatgtca
     2821 ctttcttgtg taatacagtg gtattcgatg gggaagaact tcatgcgagc tattatcagt
     2881 cgttattatc tcaggtacgg gatatggtag atgcacta
//
LOCUS       DADLFA010000276         2899 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000276 DADLFA010000000
VERSION     DADLFA010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.278"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            135..564
                     /locus_tag="JQH65_003942"
                     /pseudo
     CDS             135..564
                     /locus_tag="JQH65_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            751..915
                     /locus_tag="JQH65_003943"
                     /pseudo
     CDS             751..915
                     /locus_tag="JQH65_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001436458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            1409..1987
                     /locus_tag="JQH65_003944"
     CDS             1409..1987
                     /locus_tag="JQH65_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141176.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMAYYHANYSSIDKYITDMCGLICMRHIESTCIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(2096..2354)
                     /locus_tag="JQH65_003945"
                     /pseudo
     CDS             complement(2096..2354)
                     /locus_tag="JQH65_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2338..2899
                     /locus_tag="JQH65_003946"
                     /pseudo
     CDS             2338..2899
                     /locus_tag="JQH65_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          877 a          541 c          765 g          716 t
ORIGIN      
        1 attcctgccg gtatcaacgc cgacggcatg tattgccctg aatgcggtac ggttcactgg
       61 ccggatggcg ttattccgcc tttctgatgc ttccccgttt tgccgatatt tttcagcagg
      121 gaaaccgatg gcttaactgg ctggagaagc agccggtgca gatgagccgc ctggaacatt
      181 atgccggtca ggatgaaatt ggcctgcgtt acaacagcca ccggacaaaa cgagaagaaa
      241 accttgtgat gagtggcgac gagtttatgg aaaggttctc ctggcatgtg gcggataagg
      301 ggttccggat ggtgatacgg ggtcctgagt ccggtgaagc ggcgattact ggaagatgtg
      361 gtgtacgcca taacggagac agtgagaaaa acggcgaggc aaatcacaag gagagggatg
      421 tatcagcagt gactgaaggt tgacccgctg aagtgcgagc tgtgcggagg tcagatgcgg
      481 tttacggggc tgaagagggg ttaccgtctg gcagagcagg ttatgatgca tgagccgctg
      541 gctcgaatac ggtggtgcgg ctgagagtcg cagaggggaa gttgcgtcca tgttgagggg
      601 aacggagcaa aaaaccatca gagatacatt gaatcaatca atgtaagcaa tattaagtgt
      661 ctataccgat tacttgatga tcaggttgtg ttgaataaac gcgcattctt cggtgctttt
      721 ttaatctttt ggggatttaa attcctatcg atgaactgtc gatatgaaaa agcgagcatc
      781 atactgacgt caaacaaagg ggtcgcagac tggggagaaa tgttcggaga tcacgtgctg
      841 gcaacagcga tcctagatcg gctgctacac gctgaatatc aaaggagaga gttaccggtt
      901 aaaagagaaa cgtaaagctg gagtgctgac caaaaacaca acgccaatca gtgatgatga
      961 aatggtggaa aacggacagc atcagtaacg aaagtatctt agcaggcatg aaaatcgtcc
     1021 gcatccccta aacggtcaaa atgcttctgc cgtgaaggag ggagaatgcc atctcatcac
     1081 ttatatctgg cgaatgaaac acagatggtc ggccttgccc ccaaacacac ggcggtgcaa
     1141 aatggaatgg cagaaaactt cgtcaaaacg atgaaatatg attaaatcag tgttatgttc
     1201 gaacctgacg tgttaacagc agtaaagtag tctcgcgaag gtgttcaaac attataatga
     1261 atggcatcct catagtgcgc agggttatcg cttgctgcgg aaatatctga agcgacgtac
     1321 cagtaatggg gaaaatgata gctggtttct gaaaatatag aggaaaatgt gatatatata
     1381 tcctgttaat atttaaggag aatataaaat gaaagtctcg tttaagtctt taggctatat
     1441 ttttcatgat atatataata aaaaacacac tatcgatgag ttcaacgatg tggtaagaaa
     1501 agcagtttta tcaggtaaaa ttaacgagct gaatgcatgt cataaagtgg caatttttct
     1561 tgccgaaaaa gacaatgaga taacaaagaa agacaaagcc aagataatag atacattaac
     1621 tgaaaactat tccattgaat ttcaacaatt aatgaatatt agtgagcgca cgttaaattc
     1681 atctttatat attactcctg gtgaaagtgg ttttgtcagt tttgttaatc gtgaaggaaa
     1741 aatatgtcat actgcatatg taaaatcatc agataatagc atggcatatt accatgcaaa
     1801 ttatagcagc attgacaaat atattacgga tatgtgcggc cttatttgta tgaggcatat
     1861 tgagtctact tgcattattt tttatatgct tgatgaaaaa gttttatctg ccattgctga
     1921 atttatgaat gaaaaaggat ggcgggctgc gttttgttca gcaaagaact tatataaatg
     1981 tgtttaattt tagaaaaact atttggtaaa atccaacaag agtcgagagg ttcgcgttgc
     2041 ttcagcaggg cctcagcctg ttcaaccaga ctcagttgca ggtcgtgacg cagatttagt
     2101 ggtggtggtt gattgtcaga taccagccga tgatcttata gtgcatatca tcagactttg
     2161 agaagcatat ggttttgcgg gtaagccgct taagatgtgt ccgcaggttc agattatgtc
     2221 gctctatccg ctgagtgcat ttcttgctta taacatgact ggttgaggac aataaagttc
     2281 ggtataccgg ctcaggcatc ggtcatgtag aaggcaatgc taaactggct cagcaaacta
     2341 agagcagcgc gcatccactt acccaatgcg gttgagaaaa tagccttcga tcgctttcat
     2401 gtggcgaaac aaccgggtga ggtggtggat aaaacccgcc agaatgaaca tccgcatctt
     2461 cctgttgaaa gtcgtcgtca ggcaaaggga actcgcttcc tgtggcagca cagcgataaa
     2521 tggatgaccg aatccaggca ggaaaagctg atatggctgc gtgcgcagat gaagctgacg
     2581 agcctgtgct gggcgctgaa agaactggca aaggatatct ggagcagacc atggagcgag
     2641 gaaagacgca acgactggca gagatggctg tcgctggcgg ccaacagtga cgtccccatg
     2701 atgaagaatg tggctaaaac gatagggaaa aggttgtacg gtatcctgaa cgccatgcgc
     2761 catggcgtat caaacggaaa tgcggaagca ctgaacagca agatcaggct gctgagaata
     2821 aaagccaagg gctaccgcaa ccgggagcgc tttaagctgg gagtgatgtt ccactatggg
     2881 aaactgaata tggcgttct
//
LOCUS       DADLFA010000277         2868 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000277 DADLFA010000000
VERSION     DADLFA010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.279"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..1075)
                     /locus_tag="JQH65_003947"
     CDS             complement(<1..1075)
                     /locus_tag="JQH65_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HAY6141177.1"
                     /translation="MAFKHYDVVRAASPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGAVISATSDPEYYFVVVLAGQSNGMSYGEGLPLPGTYDRPDPRIKQLARRST
                     VTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGTVGQGLHIAKKLLPFI
                     PANAGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVDKPLYKDLIGRTKAALE
                     KNPKNVLFAVVWMQGEFDFGGTPANHAAQFGALVDKFRADLADMAGQCVGGSAGGVPW
                     ICGDTTYFWKQKNESTYQTVYGSYKNKTEKNIHFVPFMTDENGVNVPTNKPEEDPDIR
                     GIGYYGSKWRDSSATWTSQDRASH"
     gene            complement(1565..1641)
                     /locus_tag="JQH65_003948"
     tRNA            complement(1565..1641)
                     /locus_tag="JQH65_003948"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1605..1607),aa:Arg,seq:tct)
     gene            complement(1655..1731)
                     /locus_tag="JQH65_003949"
     tRNA            complement(1655..1731)
                     /locus_tag="JQH65_003949"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1695..1697),aa:Arg,seq:tcg)
     gene            complement(1739..1814)
                     /locus_tag="JQH65_003950"
     tRNA            complement(1739..1814)
                     /locus_tag="JQH65_003950"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1779..1781),aa:Ile,seq:cat)
     gene            complement(1855..2820)
                     /locus_tag="JQH65_003951"
                     /pseudo
     CDS             complement(1855..2820)
                     /locus_tag="JQH65_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403604.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
BASE COUNT          646 a          851 c          693 g          678 t
ORIGIN      
        1 aatggctcgc cctgtcctgt gacgtccagg tggctgagct gtcacgccat ttcgaaccgt
       61 aataaccgat accccgaatg tccgggtctt cttccggttt gttcgtcggc acattcaccc
      121 cgttctcatc ggtcatgaac ggtacgaaat ggatattctt ttccgtttta tttttatagc
      181 tgccgtacac cgtctggtac gtggattcgt tcttctgctt ccagaaatac gtcgtgtccc
      241 cgcatatcca gggaacaccg ccagcagagc caccgacgca ctggcctgcc atatccgcca
      301 ggtctgcacg gaatttatca accagcgcac caaactgtgc ggcatgattt gccggcgtac
      361 cgccaaaatc aaattccccc tgcatccaca ccacggcaaa cagcacattt ttcgggttct
      421 tctccagtgc cgcttttgtt cgaccgataa ggtccttata cagcggcttg tccacacccc
      481 agcgggttga attctccgag gcaccactcg cgtcactgta tgtgccatca gctccggtgg
      541 tgaacgctga accaccacga cagcacggaa ccagcagaat gcccgcattc gccggtataa
      601 acggcagcag ttttttggcg atatgcagcc cctgccccac ggttccgtac tgcccctttg
      661 acaggtccgc tttcggatgg ttaagacggc tcatgtcctg cacatcatgc agacaatggt
      721 ccgccggaat gatgtcgtta tatttgcatg ctgcaccgcc cggtgtcacc gtactgcgac
      781 gcgccagttg cttaatacgc gggtccggac ggtcatatgt ccccggcagc ggaaggcctt
      841 caccatacga catgccgttt gactgccctg ccagaaccac aacaaagtaa tactccgggt
      901 cgctggtggc gctgattact gcaccttctg ctgcaatcgc ctgcatcaga gtataagggg
      961 ttatggccac cggactacca aacggctgcc agccctcttt cagtttatgt gtcagctttt
     1021 ccgcaagatc tgacggcgac gccgccctga caacatcata gtgtttaaaa gccatgaatc
     1081 ctcccggccg ggataatatt gtgagtaaaa tgaggagcgg gctgaagtcc ggaagttaca
     1141 ggacaatggc agaagagaga cgacagcccg caattcgaaa aagaccgcgc agttgcgcag
     1201 agtgactact atggggtatt attcgccagc tgaaatatta cttcacgttt tattgtttat
     1261 tccttgccgc ccgcgtctcc cagcgcgggc tttttttgcc cacaagaaag cccctccgga
     1321 gaggggctgg agagtggcgc tatgtgccat tgcatggtgc cgggtgcctc ccggtgaatt
     1381 cagtaccagc acctgaatcc gcgattatcc catataccta ctcgctgatt gcccctccgc
     1441 acagggggat tcaccatgcc agtttctttt aacaaactcc ccgcaaacca gacaacagtc
     1501 aaccgtctga attgtgagac atttaaaaaa aaagcccgca aaagcgagcc agggaaaata
     1561 agtgtggcgc gttgtactgg attcgaacca gtgaccgatt gcttagaagg caattgctct
     1621 gtccggctga gctaacaacg catgatgcag ataatggacc gccatcgggg acttgaaccc
     1681 cgcgcagcca gcttcgaagg ctggcgctct ttcctgatga gctaatggcg gtatgtgatg
     1741 gtggcccttg ctggatttga accagcgacc tggcgattat gagtcgctcg ctctcaccac
     1801 tgagctaaag ggccgggagc cgcataataa cgacgcgtaa ttaattcttc aatatcatcc
     1861 gttctggctg actaaatcct gtacttcccg aaccgtctgc tcaaaacgtt cagtctccag
     1921 ctcaacgcca attgcacgac gcccgagcgc cagtgccgct ttcactgtcg aacccgaccc
     1981 catgaaaaaa tctgcaatca ggtcacccgg acgactgcta gcgctgatta tctgctgcag
     2041 catttctgcc ggtttttcgc acggatgttt cccgggatag aactgcaccg gtttatgcgt
     2101 ccacacatcg gtatacggca cctgcaccgt cacgccaaaa taccgccgca gatgtttata
     2161 ttcactcagc agctccgcat actgccggtt cagtgaggta tacgtatcca ccagctggtg
     2221 gtggagcttt tccagttcac cacgccgatg cttctcttct gccacccggg caaacagcgc
     2281 ctgtaatttc agataatcgc tttcattcgg caactgccac tgactggcac tgaaccagtg
     2341 cgacaccatg tttttctttc ctgtggcatc tgcaatctgt tttgccgtta tccccagggc
     2401 agctcgcgca tcacgaaagt aagaaatcag cggggccatc acatgctgtt tcagtgcact
     2461 gcccttcgcc tcatagccat catctttcgg gcggtacggc ccctgataat gttccgcgaa
     2521 cagaatgcgc tctgtggcgg ggaaatacgc ccgcaggctt tccttgttgc atccgttcca
     2581 gcgtccggac ggcttcgctc agataatatg gttcagcaca ctgaagcgtt cacgcatcat
     2641 gatttcgata tcagatgcca ggcgatgacc acagaacagg taaagacttc cggcaggttt
     2701 cagcacccgc cagaactgcg ccagacactg ctccagccac ttcaggtaat catcgtcgcc
     2761 cttccactgg ttatcccagc cctcaggctt cactttaaag tacggcgggt ccgtgactat
     2821 caggtcaaca gaattttcgg gtaacgacca gataaattcc aggcagtc
//
LOCUS       DADLFA010000278         2816 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000278 DADLFA010000000
VERSION     DADLFA010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.280"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            176..1150
                     /gene="cysB"
                     /locus_tag="JQH65_003952"
     CDS             176..1150
                     /gene="cysB"
                     /locus_tag="JQH65_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HAY6141178.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            1445..1609
                     /locus_tag="JQH65_003953"
     CDS             1445..1609
                     /locus_tag="JQH65_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HAY6141179.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            1612..1779
                     /locus_tag="JQH65_003954"
     CDS             1612..1779
                     /locus_tag="JQH65_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141180.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            1893..1988
                     /locus_tag="JQH65_003955"
     CDS             1893..1988
                     /locus_tag="JQH65_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141181.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            2152..2816
                     /locus_tag="JQH65_003956"
                     /pseudo
     CDS             2152..2816
                     /locus_tag="JQH65_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Catalyzes the conversion of citrate to isocitrate;
                     internal stop; incomplete; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
BASE COUNT          732 a          646 c          656 g          782 t
ORIGIN      
        1 tcgcgaaagc ggcccttttt tattgcatat tattttttct tcacacctat acactaaggc
       61 tataaatgat atagtggtta tagttagcac cttttttatt attaaatcgt attagtcacc
      121 cgccaggtgt gacgaaaaaa cgatgttctg atggcgtcta agtggatggt ttaacatgaa
      181 attacaacaa cttcgctata ttgttgaggt ggtcaatcat aacctgaatg tctcatcaac
      241 agcggaagga ctttacacat cacaacccgg gatcagtaaa caagtcagaa tgctggaaga
      301 cgagctaggc attcaaattt tttcccgaag cggcaagcac ctgacgcagg taacgccagc
      361 agggcaagaa ataattcgta tcgctcgcga agtcctgtcg aaagtcgatg ccataaaatc
      421 ggttgccgga gagcacacct ggccggataa aggctcactg tatatcgcca ccacgcatac
      481 ccaggcacgc tacgcattac caaacgtcat caaaggcttt attgagcgtt atcctcgcgt
      541 ttctttgcat atgcaccagg gctcgccgac acaaattgct gatgccgtct ctaaaggcaa
      601 tgccgatttc gctatcgcca cagaagcgct gcatctgtat gaagatttag tgatgttacc
      661 gtgctaccac tggaatcggg ctattgtagt cactccggat cacccgctgg caggcaaaaa
      721 agccattacc attgaagaac tggcgcaata tccgttggtg acatatacct tcggctttac
      781 cggacgttca gaactggata ctgcctttaa ccgcgcaggg ttaacgccgc gtatcgtttt
      841 cacggcaacg gatgctgacg tcattaaaac ttacgtccgg ttagggctgg gggtaggggt
      901 cattgccagc atggcggtgg atccggtcgc cgatccggac cttgtgcgcg ttgatgctca
      961 cgatatcttc agccacagta caaccaaaat tggttttcgc cgtagtactt ttttgcgcag
     1021 ttatatgtat gatttcattc agcgttttgc accgcattta acgcgtgatg tcgttgatgc
     1081 ggctgtcgca ttgcgctcta atgaagaaat tgaggtcatg tttaaagata taaaactgcc
     1141 ggaaaaataa tttcttgcgt tattttcggc accttttatg tagcgaaggt gccggaatat
     1201 attctctttt gttacttatt tttataccgt gcacaatatt tttatccctc atatttatag
     1261 ggtaaattac ctgtaaagta gtgtctaatt acccgcctcg caaattccag ttttcaaata
     1321 tctatttcag gtcaaaagat tgaattattc actggagacg attcgtaaat tcgctggatt
     1381 tttgactaaa gtttctttag gatttgtctc atcgtatgat taattacacc aatcgttcaa
     1441 cgtcttgctg ataagtgatg gcgattatat gaggttagca atgccttctg gaaatcagga
     1501 accccgccgc gatcctgaat taaaacgtaa agcctggctg gcggtttttc ttggttctgc
     1561 acttttctgg gtggttgtcg cactgctgat ttggaaagtg tggggataac tatggtcggt
     1621 caggagcaac tggagtcgtc accattatgc cagcatagtg acaatgaaac agaaacgaaa
     1681 cgggaatgtt ccgtcgttat tccagacgac tggcaactaa catcgcagca gcaagccttt
     1741 atagaactgt ttgctgaaga tgatcagccg aaacaataat tatcatcatt cttattaccc
     1801 atttttaatg aattaaaggg cttttaatac accgcagcaa taacagcttg agttatctca
     1861 acacaaaata ataaccgtta agggtgtagc ctatgatcaa cacaaatatg aaatattggt
     1921 cctggatggg cgcgttttct ctgtcgatgc tcttctgggc cgaactcctc tggatcatta
     1981 ctcactgatc cttgaccccg ctgcggcggg gttgtcattt gctttgccac aaggtttctc
     2041 ctcttttatc aatttgggtt gttatcaaat cgttacgcga tgtttgtgtt atctttaata
     2101 ttcaccctga agagaatcag ggcttcgcaa ccctgtcatt aaggaggagc tatgtcgtca
     2161 accctacgag aagccagtaa ggacacgttg caggccaaag ataaaactta ccactactac
     2221 agcctgccgc ttgctgctaa atcactgggc gatatcaccc gtctacccaa gtcactcaaa
     2281 gttttgctcg aaaacctgct gcgctggcag gatggtaact cggttaccga agaggatatc
     2341 cacgcgctgg caggatggct gaaaaatgcc catgctgacc gtgaaattgc ctaccgcccg
     2401 gcaagggtgc tgatgtagga ctttaccggc gtacctgccg ttgttgatct ggcggcaatg
     2461 cgcgaagcgg ttaaacgcct cggcggcgat actgcaaagg ttaacccgct ctcaccggtc
     2521 gacctggtca ttgaccactc ggtgaccgtc gatcgttttg gtgatgatga ggcatttgaa
     2581 gaaaacgtac gcctggaaat ggagcgcaac cacgaacgtt atgtattcct gaaatgggga
     2641 aagcaagcgt tcagtcggtt tagcgtcgtg ccgccaggca caggcatttg ccatcaggtt
     2701 aacctcgaat atctcggcaa agcagtgtgg agtgaattgc aggacggtga atggattgct
     2761 tatccggata cactcgttgg tactgactcg cacaccacta tgatcaacgg ccttgg
//
LOCUS       DADLFA010000279         2812 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000279 DADLFA010000000
VERSION     DADLFA010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.281"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(39..114)
                     /locus_tag="JQH65_003957"
     tRNA            complement(39..114)
                     /locus_tag="JQH65_003957"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(79..81),aa:Val,seq:tac)
     gene            374..1789
                     /gene="gltX"
                     /locus_tag="JQH65_003958"
     CDS             374..1789
                     /gene="gltX"
                     /locus_tag="JQH65_003958"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAY6141182.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGR
                     TRSIERINKALAFIAERENQQ"
     gene            complement(1840..2211)
                     /locus_tag="JQH65_003959"
     CDS             complement(1840..2211)
                     /locus_tag="JQH65_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HAY6141183.1"
                     /translation="MTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGGRARLILV
                     DTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGVRAPAYRQIINAIDNMTDIEQEKVA
                     QFLSREGIRNFLARLDIDESA"
     gene            complement(2234..2578)
                     /locus_tag="JQH65_003960"
     CDS             complement(2234..2578)
                     /locus_tag="JQH65_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HAY6141184.1"
                     /translation="MTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKARLVHV
                     NEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLLREGII
                     GLLQRLGIRDSK"
BASE COUNT          691 a          691 c          703 g          727 t
ORIGIN      
        1 caatttcttg ctacacggcg gagactacat aaagtagttg gtgggtgatg acgggatcga
       61 accgccgacc ccctccttgt aagggaggtg ctctcccagc tgagctaatc accccccgct
      121 gtgtggagtc gcattatagg gagagttcaa aatgagtcaa cgcattttct aaagaaattg
      181 ttcgttcgtc gtaaatttaa gcaagatgat cgcaaaatag accgtgttgc gcaatttgtc
      241 aacgaaaaca ataatgcgta aggtagaaac ccgaactaca ttgaggaatc aggcgggagt
      301 gatagaatat cgcccactca atttttccag gatttgccgg ttgtcggcat ctttctaaac
      361 gtaaggccat ttcatgaaaa tcaaaactcg cttcgcgcca agcccaacag gctatctgca
      421 cgttggcggc gcgcgtactg ccctttactc ctggcttttt gcacgtaacc acggcggtga
      481 gttcgtgctg cgtattgaag acaccgatct tgagcgttcc acgccggaag ctatcgaagc
      541 cattatggat ggcatgaact ggctgagcct ggagtgggat gaaggtccgt actaccagac
      601 caaacgtttt gatcgctaca acgcggtgat cgatcagatg ctggaagagg gcactgctta
      661 taaatgctat tgctctaaag agcgcctgga agcgctgcgc gaagagcaaa tggcgaaagg
      721 tgagaagccg cgttatgacg gtcgctgccg ccacagccat gagcatcacg ctgatgatga
      781 accgtgcgtt gtacgttttg ctaacccgca ggaaggttct gttgtttttg acgatcagat
      841 ccgtggtccg atcgagttca gcaaccagga actggacgat cttattattc gccgtaccga
      901 tggttcccca acctataact tctgtgtggt tgtcgatgac tgggatatgg aaatcaccca
      961 cgttatccgt ggcgaagacc atatcaacaa cacgccacgc cagatcaaca ttcttaaggc
     1021 cctgaaagcg ccggtgccgg tttacgcgca cgtttctatg atcaacggcg atgacggtaa
     1081 aaaactgtcc aaacgtcacg gggcggtcag cgtaatgcag tatcgtgatg acggttattt
     1141 gccagaagca ctgctgaact atctggtgcg tctgggctgg tcccacggcg atcaggaaat
     1201 cttcactcgt gaagagatga tcaaatactt caccctgaat gccgtcagca aatctgccag
     1261 tgcgttcaac accgacaagc tgctgtggct gaaccatcac tacattaacg cgctgccgcc
     1321 ggagtatgtt gctactcact tacagtggca cattgagcag gaaaatatcg atacccgtaa
     1381 cggcccgcag ctggctgatc tggtgaaact gctgggcgaa cgctgcaaga cgctgaaaga
     1441 gatggcacag agctgccgtt atttctacga agattttgct gagttcgatg ccgacgccgc
     1501 gaaaaaacat ctgcgtccgg tagcgcgtca gccgctggaa gtggttcgtg acaaactggc
     1561 cgcgattact gactggaccg ctgaaaacgt tcatcacgct attcaggcga cggcggatga
     1621 gctggaagtg ggtatgggta aagttggtat gccgctgcgt gtcgccgtaa ccggtgcggg
     1681 gcagtctccg gcactggatg ttaccgttca cgcaattggt aggacccgca gtattgagcg
     1741 tatcaacaaa gcgctggctt ttattgctga acgcgaaaac cagcagtaat tagcgcataa
     1801 aagataaacg gcaggagata atatcctgcc gttttttatt tatgctgatt cgtcaatatc
     1861 gagacgggcg aggaaattgc gaattccttc acgtgacaaa aattgcgcga ctttttcctg
     1921 ctcaatgtct gtcatattat ctatcgcgtt gatgatttgt cgatacgccg gggcgcgtac
     1981 tccaggagcg tattcagcaa ttctctcttc agcttccatc aacattggtg tgttatggaa
     2041 ggctggcgtg ttctgaataa actcgcaaac ttgcgtatcg accagaataa gtctggcacg
     2101 accgcctttc acgccgggaa atttttccgt tttccagcct ttttctctga tccaacggtt
     2161 aacagtttgt ttagccacac cgaggcattc agccagttct tcggtggtca ttttattgcg
     2221 taatcttttc atattatttg ctatcgcgaa tccctaagcg ttgtaacaat ccgataatcc
     2281 cttcccgcag aagcaaggat gtaaactgtt tttgttcaac tggcgtcatc tctttcgcca
     2341 gtgtcaccag caatacttca agcggcgcat caccagaaag ggcaggcgct tctccctgac
     2401 cttctggacg ttcagcattg cgaatatatt cacgaacctg ttcattgacg tgaaccagtc
     2461 gggctttgcc accctggacg ccaggttttg gtgacgttgt ccagccttcc ttgcgtaccc
     2521 atttattaat ggtctggcgg ctatagccgg tcagtctggc aagttcatct ggtgtcatcc
     2581 tctccttgaa cataacaatt ccctgaatgt atgtgggtct attagtagtt cattttataa
     2641 caccacttta caggaaaccg tgacatgaat aactactgaa tgcgagcggt tttccaaaat
     2701 tgttcatttg cctgattttt cagcgattga aactacacgt tgcaatttgc cgttgacaca
     2761 ttcgggcgga attcatatga tgccgcccgt caactcgaca agctttacgt ga
//
LOCUS       DADLFA010000280         2750 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000280 DADLFA010000000
VERSION     DADLFA010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.282"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..1979)
                     /locus_tag="JQH65_003961"
     CDS             complement(<1..1979)
                     /locus_tag="JQH65_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HAY6141185.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTANIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNV"
     gene            complement(2023..2604)
                     /locus_tag="JQH65_003962"
     CDS             complement(2023..2604)
                     /locus_tag="JQH65_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HAY6141186.1"
                     /translation="MKQQEEHNNKIDLLEKQQAQLKSQLETIQKQQTGIISSTKTLTH
                     VIKSVKDQQNTFIFTEFNPAKTKYFILNNGSVALAGRVLSIDATENGSVIHISLVNLL
                     STPISNIGFNATWGGEKPVDAKEFARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVS
                     PNNLGYLKLAINMENIQFDNLPSAENRQKRSKK"
BASE COUNT          641 a          656 c          606 g          847 t
ORIGIN      
        1 ttaacgttgg tgattttgct ggtggtctcg gtgccatgag ctgccgtgaa tacaccattg
       61 tccttatccc atttcagcgc atcctcacca agattagtca ccgtctcagt cagattagag
      121 atgttggtgg tgttagtggc aatattggaa gtgttattgg cgatattggt ggtattcgtc
      181 gccacagcat cgttggtggt tttcagctga gagccgttaa cagcatcagt gctgtcagcc
      241 gtcaggtcgg catctttaac gttggtgatt ttgctggtgg tatcctggcc gtgagctgca
      301 ctgaatgcct ttttagtttc gttccagagc agggcatcag cctgaaggtc accaacggaa
      361 tccgtcaggt tggtgatatt ggtggtattg gtggcaatat ttgccgtatt cgtggcaatg
      421 ttgctggtat tagtagcgat attggcggtg ttggcttcga catcgtcatt ggtagctttc
      481 agttgggaac cgttaaccgc atccttactg gaggctgaaa tcgtgccatc cgcaacatcg
      541 gtgattacgc tggcagttgg gctactagta ccatgagcag cgctgtattt gcctttggtt
      601 tggtcccatt gcagggtgtt ttcatcaagt acagcgagcg cagcccctac gttatttttg
      661 ctaccatttt ttaaattgta ggttggtgct gtaacagtac cgtcatcaac atctactgca
      721 gcccctcctc caagcctttt tgctaccgag tcgctaatgg cataaagctg tgaaccatta
      781 atggcatcgt aactgtcaga cttgatcgcg ccatttttca cattaacgat cttgcgtttt
      841 aatgaactat tgccgacaga gacggtattg tctaaatccg caatggaatc tgcgcccagc
      901 gcgacagagt taataccgtt ggcactgcta ttactaccaa gcgcaagact cttatcacca
      961 gttgcattac tggctgtgcc taaggcaatg gtgtttaagc ctctcgactg gctaccatta
     1021 cccagagcga tggcatccac gccatcagct ttgttgccct ggcctatggc gattgcatta
     1081 tctgccagag cctggctgcc atttcctaat gcgatagtat tgactttatc ggcaaggctt
     1141 ccgaacccca ttgccatgct attagtccct tttgccttcg cattattgcc aatagcagtt
     1201 gcattttcag cggcttcggt ctcagcacct atcgcaatag cgttcgctgc gctggcaagt
     1261 gattgtctgc caaatgcgag gctgtcagta ccgctagcta cactgctacg ccccagggca
     1321 attgcgtttt cttcagatgc gacgctactt gctcccagcg ccattgaata cgcttttgac
     1381 gcattggcgg tgtcgccaag agcaatactc ataatctcag tagctttagt ggcatttccc
     1441 agggcaatgg cgtttgctcc ttccgctttg gaagagtccc ccatagcaat agacaattta
     1501 cccaatgctt ttgaataacg gccgagggcc attgagtatt ggcccaatga atacgaagag
     1561 gcacccagtg ctatacttct atcgccttct gatattgcac taaccccaaa ggccattgat
     1621 gcaccaccaa tcgcatgggt ttttgacccg atggcagagc tatattggcc ttcagctata
     1681 gcgtcataac ccacagcagt actggaacca ctggtgttca taaaagtatt tgctttcgcc
     1741 cctttgccta tagcaaccca gccgtcgcca gctgatccac taccgtaatc aacaccctga
     1801 ccgttgtcat tcccggcatt cgccaatgcc ccaaacgacg acaacattcc acccgcaacc
     1861 agcgcagaaa ttaacagctt actgcgccca gatttcttgc cacggctttt tgccgtttcg
     1921 ctggtaacag tataattccc tgtcgcaggg ttccagataa ctttaaatat tttgttcatt
     1981 aatgatgctc gctattcctt gttataagtg tactaactgc atttattttt tgcttctttt
     2041 ctgccgattt tcagcagagg gaagattgtc gaactgaata ttttccatgt tgatggctaa
     2101 cttcagatat cccaggttat tgggcgagac acccttcagg gtcaaattaa tgtcttgcca
     2161 ttgacctggt aataatttca atgtggattt cattgatgtg ttgaaaagta attgttgcca
     2221 ccgggcaaac tctttggcat cgacaggttt ttcaccaccc catgtcgcat taaaaccaat
     2281 atttgagata ggtgtactta ataagttgac cagtgaaata tgaataacac tgccattctc
     2341 tgtggcgtca atggataaca ctcgacccgc taaagccacc gaaccattgt tcaggatgaa
     2401 atacttcgtt tttgctgggt taaactcggt aaaaataaaa gtgttttgtt gatctttaac
     2461 ggatttaatt acgtgagtta aagtctttgt actgcttatt atgccagttt gttgtttttg
     2521 aatagtttcc agttgactct ttagctgtgc ttgttgtttt tcaagtagat caattttatt
     2581 gttatgttct tcttgttgtt tcaataaact actttctggc ttgccttctt caatgtagtc
     2641 acatcccgtt aatgcgcaga gaactgaaac agtgacagca ctgaaaaaaa tcttcttcaa
     2701 gttcatgtaa aaatccttat ttcctccgac cagcgatcaa tgccggtaga
//
LOCUS       DADLFA010000281         2741 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000281 DADLFA010000000
VERSION     DADLFA010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.283"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(230..2608)
                     /gene="ppsA"
                     /locus_tag="JQH65_003963"
     CDS             complement(230..2608)
                     /gene="ppsA"
                     /locus_tag="JQH65_003963"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HAY6141187.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNGRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
BASE COUNT          654 a          761 c          683 g          643 t
ORIGIN      
        1 aagaggtggt ggcttttttt agccgtaaac gggtcgcaaa atataaatat cctgaacata
       61 tcgtggtaat cgaaaaacta ccgcgcactg cctcaggtaa aatacaaaag tttttgttaa
      121 gaaaagatat tatgcggcga ttaacgcagg atgtctgtga agagattgaa taagtttcat
      181 cttcggggat cacataaacc cggcgacaaa acgccgccgg gtatttattt tatttcttca
      241 gttcagccag gcttaaccag gtttgcacca cggtgtccgg attcagagac aggctatcga
      301 tcccctcttc catcaaccat gcggcaaaat cttcgtggtc ggacggaccc tgaccgcaaa
      361 tcccgacata tttgccctgt ttcttcgcgg cacggatagc catcgacagc aatgctttca
      421 ccgcatcgtt gcgctcatcg aacagttcag acaccacgcc ggagtcacga tccagaccga
      481 gcgccaactg cgtcatgtcg tttgagccaa ttgagaagcc gtcgaaatat tcgaggaact
      541 gctcggccag caaggcgttg gacgggattt cacacatcat gatgattttc agcccgttct
      601 caccacgctt cagcccctga cgcgccagtt cttcaaccac cgctttcgcc tggtctacgg
      661 tacgcacgaa cgggatcatg atctcaacgt tggtcagccc catgtcgttg cgcacacgtt
      721 tcactgcttc acactccagc gcgaagcagt cgcggaagct gtcggaaaca tagcggcccg
      781 cgccacggaa gccgagcatc gggttctctt catctggctc gtaacgctca ccaccgacca
      841 ggttggcata ttcgttcgat ttaaaatcag agagacggac aatgacgcgc ttcggataaa
      901 acgcggcacc cagcgtcgcg atcccttcag tcagacgacc aacgtaaaat tcacgcggag
      961 aatcaaaacc tttcatcatc tcgcggattt cgttttgcaa ctgcggttcc tgatcgtcaa
     1021 actcaagcag tgcgcgtggg tggacgccaa tcatacggtt gatgataaat tccagacgcg
     1081 caagtcccac gccttcgttc ggcagacagg cgaagtcgaa agctcgatcc gggttaccga
     1141 cgttcatcat cactttcaac ggcagatccg gcatggtttc tacgctggag cttttcacgc
     1201 taaattccag caactccgca tagacgtaac cggtatcacc ttcggcacaa gaaacagtga
     1261 cgttctcacc gtctttcatc cgttctgttg catcaccaca gcccacgacc gccggaatac
     1321 ccagttcacg agcgatgatc gccgcgtgac aggtacgacc gccacggttg gtgactatgg
     1381 cagatgcttt cttcatgatc ggttcccagt ccgggtcggt catgtcagtg accagcacgt
     1441 caccaggttc gatgcggttc atttcgctga tgtcatggat gactttcacc ggacccgcac
     1501 cgatgcgatg accgatagca cggccttcgg cgataatctt accctgtgaa tgcagcgtat
     1561 aacgctccat gacctgaccg cgtgagcgca cggtttccgg acgcgcctgc acaatgaaca
     1621 gtttgccggt gtggccatct ttcgcccact caatatccat cgggcgaccg tagtgtttct
     1681 caatttgtac ggcctgtttt gccagttcct gcacttcttc gttggtcagc gagaagatgt
     1741 cacgctgttc ctgcggtacg tcttcgattt taacctgctt gccgtgctcc tgggtcggcg
     1801 cgtaaaccat gcggattttt ttcgacccca tggtgcggcg cacgatagcc gggcgattcg
     1861 ccgccagtgt cggtttatgc acgtaaaact catccgggtt aaccgccccc tgcacgacca
     1921 tctcaccaag gccccatgcg gaagtgataa acaccacctg gtcaaagccg gattcggtat
     1981 caatggagaa catcacgcca gaagatgcga ggtcggagcg caccatccgc tgaacaccgg
     2041 cggagagcgc cacgccacgg tgatcgtaac cctggtgcac acgataagag atggcgcgac
     2101 cgttaaacag agaagcaaat acatgtttca ctgccacgag aacggcgtca aacccctgaa
     2161 cgttgaggaa ggtttcctgc tgaccggcaa aggaagcgtc cggcatatct tctgcggtgg
     2221 cggaggagcg caccgcaaaa gaggcgtttt cgtcatcggc ggaaagctga gcataggctt
     2281 cgcggatggc gttttccagc tcaggctgga agggagtgtc gataatccac tggcggattt
     2341 gcgcgcccgc tttcgcaagc tgagtaacat cgtcaatatc cgttttatcc agcagttcat
     2401 aaatgcgctg gtttacgccg ctttggtcca gaaactggtt aaacgcgtcg gcggttgtgg
     2461 cgaaaccatt cggaacggaa acgcccattc cggaaaggtt agtaatcatt tcacccaggg
     2521 aggcattttt gcccccaacc ctgtctacat cattcatgcc gagttggttg taccaaagca
     2581 ccagcggtga cgagccattg ttggacatcg aacaatcctt ttgtgataaa tgaacggttt
     2641 gagaaacaca tttctgcgca tttatctttg catatttaac cggatgaaaa aaacggtgaa
     2701 tcgttcaagc aaatatattt ttttactttt taagactgat c
//
LOCUS       DADLFA010000282         2738 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000282 DADLFA010000000
VERSION     DADLFA010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.284"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            1..134
                     /locus_tag="JQH65_003964"
                     /pseudo
     CDS             1..134
                     /locus_tag="JQH65_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            384..1250
                     /gene="ospB"
                     /locus_tag="JQH65_003965"
     CDS             384..1250
                     /gene="ospB"
                     /locus_tag="JQH65_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HAY6141188.1"
                     /translation="MNLDGVRPYCRTVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            1580..2320
                     /locus_tag="JQH65_003966"
     CDS             1580..2320
                     /locus_tag="JQH65_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAY6141189.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAEDSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDENMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
BASE COUNT          902 a          497 c          515 g          824 t
ORIGIN      
        1 cggtaagaga agttcatcat acttggcgca gttacgcgat gtttctgcta gaggtgatgc
       61 cgtaaagaac ggcagaacat taccggaaca agatagcggt ctacctgcac tggtatcaga
      121 cccggggctt ccccggatga tctccacggt actttaggta ctgtgtctat attatgtagg
      181 taggatcaat taactttgta gttgaaattc tttcattgaa ataatgaata aaaaagacaa
      241 accgcattaa acaatgccag aatataattg catttttgtt ccgttttttt aaatcatagt
      301 atacaaagaa gcttgcatac tatatgaaaa tataacaccc tttactatga tgtttataaa
      361 acaatatatg gagtcatgta ggtatgaatt tagatggtgt tagaccatac tgtagaacag
      421 tcaataaaaa gaatgaaagc atatcagata ttgcatttgc acatataata aaaagggtaa
      481 aaaattcatc atgtactcac ccaaaagcag cattggtttt tttaggagag aaaggttttt
      541 gtgatagcaa tgatgttcta tctattatgg gacaacaaat accaagagta tttaagaaca
      601 agatgttata tgattatgtt tttaaaaatg aaaaaagtaa aaatgatttt ctaaaaatgg
      661 ctgaatcatg gctaccacag agtgaaccaa tagtaataaa taatgatgat gacgcattga
      721 atgctgctgc ttatttttct gtaaaaaaag cgaaaataaa aacagtaaac gatactgatt
      781 ttaaagagta taataaggtt tatattcttg ggcacggtag tcctggttct catcaattag
      841 gccttggttc ggaacttatt gatgtacaaa caatcatttc aagaatgaaa gactgtggta
      901 ttctaaatgt gaaagatatc cgttttactt catgcggctc cgctgataaa gtggctccta
      961 aaaattttaa caatgcccct gctgaaagtc tttcttgtat ccttaactct ctgccttttt
     1021 ttaaggaaaa agaatctttg ctagagcaga taaaaaaaca ccttgaaaac gatgagtcat
     1081 tgagtgatgg tctaaaaata tccggctatc atggatatgg agttcactat ggtcaagagc
     1141 tttttcccta ctcacattat cgttcaactt caattcctgc tgatccggag catacagtaa
     1201 aaagaagctc tcagaaaaag acttttatta ttaataaaga actggattag tataaaattt
     1261 ttaacctata gttaattgtt tgcatttaag atgaagttat ttttttcttt agtagaaaag
     1321 tgcttcccta tgagggaaaa tcacaaatca tcataataca gagactaaac gatacgaaca
     1381 aatagataaa aaacatcatt cttttactac attatttttc gtgaatatga aaaataatgt
     1441 tattacttta atataagact attttttgtt tttccatcac tctgttcaaa tttttccgca
     1501 tgacttgtgt tttttgtaac acagctcgtt ttttacagct gaccaaaatc atcaattaat
     1561 tatgctaagg aaataaatta tgaaaaccaa aaactttctt cttttttgta ttgctacaaa
     1621 tatgattttt atcccctcag caaatgctct gaaggcagaa ggttttctca ctcaacaaac
     1681 ttcaccagac agtttgtcaa tacttccgcc gcctccggca gaggattcag tagtatttca
     1741 ggctgacaaa gctcattatg aattcggccg ctcgctccgg gatgctaatc gtgtacgtct
     1801 cgctagcgaa gatgcatact acgagaattt tggtcttgca ttttcagatg cttatggcat
     1861 ggatatttca agggaaaata ccccaatctt atatcagttg ttaacacaag tactacagga
     1921 tagccatgat tacgccgtgc gtaacgccaa agaatattat aaaagagttc gtccattcgt
     1981 tatttataaa gacgcaacct gtacacctga taaagatgag aacatggcta tcactggctc
     2041 ttatccctct ggtcatgcat cctttggttg ggcagtagca ctgatacttg cggagattaa
     2101 tcctcaacgt aaagcggaaa tacttcgacg tggatatgag tttggagaaa gtcgggtcat
     2161 ctgcggtgcg cattggcaaa gcgatgtaga ggctgggcgt ttaatgggag catcggttgt
     2221 tgcagtactt cataatacac ctgaatttac caaaagcctt agcgaagcca aaaaagagtt
     2281 tgaagaatta aatactccta ccaatgaact gaccccataa agctggacag cctgtatcag
     2341 gctatggagg gcccatagac aaatctaccc tatatgagca taggaggagt ctatgggcac
     2401 accacgtttt acccctgaat ttaagggatt actggaaagg ctgggacata tccctccggc
     2461 agaagcagaa aaagcttatt atgctgccat cggaaacgat gatctggcaa cctgagttca
     2521 cagataaaac attctctagg aaactcgggg cggttccgtt caccacatgc aatgtggtgt
     2581 tgcaggggaa cggtctgcac catcccctat gtcgatcaat ataacagaaa tgacaacttc
     2641 agattcaggg cacaacctaa atatatttta ggtcacctct caaatcgttt gcctgatacc
     2701 gctccatttt ttaataaaaa aatcaatcat ttttgaat
//
LOCUS       DADLFA010000283         2668 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000283 DADLFA010000000
VERSION     DADLFA010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.285"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            231..770
                     /locus_tag="JQH65_003967"
     CDS             231..770
                     /locus_tag="JQH65_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HAY6141190.1"
                     /translation="MSHGGWKEISHPQTLFTRQKGDGWTVVPKNLRIDFYTKDNDFTK
                     GLSVLSEVNKRHTEAQKGLTPSLNISKDDLKVLANARNIPQSKLEEEMMSQAIYRKEY
                     ATSNEKIKNYALYYHEQAQNIIQRHQDGLGNKNIDIAFITDKNHKKHLSDIFKVINES
                     GVKYDVIHFGACRVNREEK"
     gene            complement(996..1319)
                     /locus_tag="JQH65_003968"
     CDS             complement(996..1319)
                     /locus_tag="JQH65_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HAY6141191.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKNVNSLTDDRVLKEVMRLGALQKKLFIDGKRIGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            1592..1786
                     /locus_tag="JQH65_003969"
     CDS             1592..1786
                     /locus_tag="JQH65_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HAY6141192.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALDADEQATMCEKLHEL
                     AEELQNSIQTRFEVENSTER"
BASE COUNT          863 a          656 c          564 g          585 t
ORIGIN      
        1 aaaaaaaaac aataaaaaac accgcaaaaa tggattgtta acaaatgaaa atactcaatt
       61 cgaatctcat aacaaacata tcatcaacaa taagaaatca ccttctaaca tcatccaaaa
      121 atataaaaac acacccatca ccaaacatgg acgtaacaag tcatcgtcgt ttattccaga
      181 aagacggaat aggattgagc caaacaggaa aagcaacaca acttgtcatt atgtcgcatg
      241 gtggatggaa agagatctcc catccacaaa cactattcac gcgtcaaaaa ggcgatggat
      301 ggactgtagt ccccaaaaac ctaagaattg atttttatac aaaagacaac gacttcacaa
      361 agggactaag cgttctttca gaggtaaaca aacgccatac tgaagctcaa aaagggttaa
      421 caccatcact aaacataagc aaagatgacc taaaagttct agcgaacgcg cgcaatatac
      481 ctcaatcaaa attagaagaa gaaatgatgt cacaagccat atatagaaaa gaatacgcaa
      541 caagtaatga gaagataaaa aactatgctc tatattacca tgaacaagcc caaaatatta
      601 tacaaagaca tcaagatggc ttaggaaaca aaaatattga tatagcattt ataactgata
      661 aaaatcataa gaagcacctt tctgacatat ttaaagtaat aaatgaatca ggagtaaaat
      721 atgatgtaat tcactttggc gcttgccgag taaatagaga ggagaaataa tcatcattta
      781 ccataatccc ttaattgtac gcaccactga aacgcgctga acgcgattct gacgcagaca
      841 ccacaaaaat agatgtttta ccccggaaaa acaggaagga tgcgtcagga tcgttttcag
      901 ctcgctgcat agctatgcat gaaagcgaat ggagatcact ttgggagctt accgtgttca
      961 taccgtctgt tttcaacagt tttctccccg ggaagctaat ctgccataag cctggataac
     1021 agggcacggt gatactccgt aatagcgatc agcacctccg catactccct gtccccgata
     1081 cgcttgccgt cgataaagag ttttttctgt aacgccccca gtctcataac ttctttcagt
     1141 acccgatcat cagtcagtga gttaacgttc ttaccgagag ctgccgcccg taaaaaaccg
     1201 gaaacggtct tgccgcattc agcagccctt tttctgattt cctggtcttc ttctgccgtc
     1261 agacgaacag ggcgactgat agcccgcctg cgcgtattac ttccacttcg ttttgtcata
     1321 aaacatggct ccgtatctga catgggtgtc ggggcaaagc cctgaccagg gcaattgtaa
     1381 tagcgtgcat gtatgcgcgg tataacaatt gcacatcctg tcctgtcagc aagctcgaat
     1441 taccgacaaa ggcacggcag ttttttggca gttacagtgt tgtctgacag aaatgcccgt
     1501 caaaaagcca ggcggcagca aaacagtcga aaagtggtac gcgatataat cacataacgc
     1561 agatgacaga cagaacggag aaaacgaaaa gatgaacaag cagcagcaag ccgtactcaa
     1621 catggcggga ttcataaaaa gccagagcct gacgctgctc gaaaaactgg acgcactcga
     1681 tgctgacgag caggccacca tgtgtgagaa actacacgaa ctcgcagaag aactccagaa
     1741 cagcatacag acacgctttg aagtagaaaa tagcacagag agataacgct caggacagga
     1801 tgtcgaacgg cacaacagag cgtaaggaga aaatgtcaca taaggcgctc ttccgcttcc
     1861 tcgctcactg actcgctacg ctcggtcgtt cgactgcggc gagcggtatc agctcactca
     1921 aaggcggtaa tgcggttatc cacagaatca ggggataacg ccggaaagaa catgtgagca
     1981 aaaagccgaa ggcagggaca aaggccgcgc aggtggcgtt tttccatagg ctccgccccc
     2041 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     2101 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     2161 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     2221 cacgctgttg gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     2281 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     2341 cggtaagaca cgaattatcg ccactggcag cagccactga taaccgaatt agaggagtaa
     2401 gacttgaagt catgcgcctg ttaaggctaa actgaaagaa caagttttgg tgactgcgct
     2461 cctccaagcc agttacctcg gctcaaagag ttggtatctc tgagaacctt cgaaaaaccg
     2521 ccttgcaggg cggttttttc gtttacaggc agtagattac gcgcagaaaa aaaggatctc
     2581 aagaagaacc tttaattttt tttcacactt ggtatcctac tttaacccat aaaataaaca
     2641 cttacaccga cacgccctta aacaaaag
//
LOCUS       DADLFA010000284         2637 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000284 DADLFA010000000
VERSION     DADLFA010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.286"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            107..1555
                     /gene="cas3"
                     /locus_tag="JQH65_003970"
     CDS             107..1555
                     /gene="cas3"
                     /locus_tag="JQH65_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HAY6141193.1"
                     /translation="MLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNKILLADEIHACD
                     AYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGAEGQQEAPLLGKDDY
                     PWLTHVTKTDVHSHRVATRKEVERSVSVGWLHSEQECIARIESAVSQGKCIAWIRNSV
                     DDAIQVYRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKYCSLLRASQVIV
                     CTQVIEQSVDIDLDEMISDLAPIDLLIQRAGRLQRHIRDINGQLKRDGKDERSPPELL
                     ILAPVWDDAPGDEWFGSAMRNSAYVYPDHGRIWLTQRVLREQGAIQMPHAARLLIESV
                     YGEDVVMPEGFARSEQEQVGKYYCDRARAKKYVLNFRLGYAANINDYLPEKLSTRLAE
                     ESVSLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLLEGDAFRQWCVE
                     QRQDPEMANVILVTDDESCGYSAREGLIGKVG"
     gene            1653..>2637
                     /gene="casA"
                     /locus_tag="JQH65_003971"
     CDS             1653..>2637
                     /gene="casA"
                     /locus_tag="JQH65_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HAY6141194.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQTSFAPKDQRSWDDIWEDGLEAEKLREALLSLEHAFQFGPDSPSFM
                     QDFDELKVKATFIASLLPDAPGKQTKERNTDHFIKRDTTQHLCLHCVPLALFSIQLNA
                     PIGGRGYYPGLRGGGPLTTLIELLEYQGNQQTPLWRKLWLNVMPQDEADLPLPKTFDD
                     LVFPWLAPTRTSELDGAVVTDEQVNKLQAYWGMPRRIRIDFKTTSIGNCDICGRQSDA
                     LLGLMSLKNYGVQYVMWRHPLTPYRLPLKEGGDFYSVKPQPGGLIWRDWLGL"
BASE COUNT          648 a          597 c          732 g          660 t
ORIGIN      
        1 taatgaatca ctctggtccg gtgatttaat cgggtcagaa gaaccggatg aacaagcttt
       61 cagccagggt tgtgcggcct agtttgccga cagtaacaag aaggcgttgc tggctgaaat
      121 tggcgtcggc accctggatc aggcgatgat ggcagtgatg ccgtttaaac ataataatct
      181 gcggcttctg gggctgagta acaaaatcct gctggctgat gagatccacg cctgtgacgc
      241 ttatatgtcg tgcattcttg aagggctgat cgagcggcag gcgcgtggcg gaaacagcgt
      301 tattttgctt tctgctacgt tatctcaaca gcagcgcgac aaactcgtcg ccgcctttgc
      361 gcgtggtgca gagggccagc aagaagcccc actcctggga aaagatgatt acccctggct
      421 gacgcatgtc acgaaaaccg atgtacattc gcaccgggta gcgacgcgca aagaagttga
      481 acgtagcgtg agcgtgggtt ggctccatag tgaacaagag tgtattgcgc gtatcgaatc
      541 ggcggtaagt caggggaaat gcatcgcctg gattcggaat tctgttgatg acgctattca
      601 ggtgtatcgt cagctgcttg cccgcggcgt cattcctgct tccagccttt cactttttca
      661 tagccgcttt gcttttagcg atcgccagcg aattgaaacg gaaacgctgg cacgctttgg
      721 taaatattgt agtttactga gagcgtctca agtcattgtt tgtacacaag taattgaaca
      781 aagcgtggat attgacctgg acgaaatgat ctccgacctg gcccccattg atttgctaat
      841 ccagcgagcg gggcgattac agcggcatat ccgcgatatt aatggtcagt taaagcgtga
      901 cggaaaagac gagcgttccc cccctgaatt gctgattctg gcccccgtct gggatgacgc
      961 tcctggtgac gaatggttcg gcagtgccat gcgtaacagt gcatatgtct atcccgatca
     1021 tgggcgaatc tggctgacgc agcgtgtgct gcgtgagcaa ggcgctattc agatgccgca
     1081 cgcagcccgg cttcttattg aatcagttta cggtgaggac gtggtaatgc cggaaggatt
     1141 tgcccgcagc gagcaggagc aagtgggcaa atattactgt gatcgcgcaa gggctaaaaa
     1201 gtatgtgctg aacttcaggc tcggctatgc cgccaatatc aacgattacc ttccggaaaa
     1261 actgtcgaca cgtctggctg aagagtctgt ttccctgtgg ctggctacct gtattgacgg
     1321 tgtggtgaag ccttatgcca ccggtgctca cgcatgggaa atgagcgttg tcagagtgcg
     1381 ccgaagctgg tggaaaaaac atcgggatga gttttcttta ctggaagggg atgcgttcag
     1441 gcagtggtgc gttgaacagc ggcaagatcc ggaaatggca aacgtgattt tagtcaccga
     1501 tgacgaaagt tgcgggtatt cggccaggga gggattgatt ggcaaggttg gttgagatgt
     1561 ctgcgacaag actctctgaa tagttgatat acagaaaagg atggaaggat ggacaaccga
     1621 cctgggatgg tcttttttac gagaggatgc gtatgaactc gttttcactt ctgacaaccc
     1681 cgtggttgcc cgttcgtttt aaagacggaa cgacaggcaa actggcacca gtcgatctgg
     1741 ctgatgaaaa tgttgtcgat atcgctgcgc cgcgggcaga tctccagggg gcggcctggc
     1801 agtttttgct ggggctacta caaaccagct tcgcgccaaa agatcaacgt agttgggatg
     1861 atatctggga agacggactg gaagctgaaa agctaaggga agcattgctg tctttagaac
     1921 atgcttttca gtttgggcca gattcccctt catttatgca ggattttgat gaattaaaag
     1981 ttaaggcaac ttttattgct tcgcttcttc ctgacgcacc aggaaaacaa acaaaagaac
     2041 gaaatacaga ccattttatt aagcgagaca caactcagca tctgtgcctg cattgtgtgc
     2101 ctttagcgct tttttcaatc cagttgaatg cacctatagg tggacgcggt tactatcctg
     2161 ggctacgtgg cggagggcct ctgacgacac ttatagaatt gctggagtat cagggcaatc
     2221 aacaaacccc tttgtggcga aagctgtggc taaacgtaat gccgcaagat gaagccgatt
     2281 tacctttacc aaaaaccttt gatgacctgg tctttccttg gcttgcaccg acgcgtacca
     2341 gcgaactgga cggtgctgtg gtaacggatg aacaagtcaa taaacttcag gcctactggg
     2401 gaatgccacg acgtatacgt attgatttta agacaacctc aataggtaac tgcgacattt
     2461 gcggtaggca gagcgatgct cttttgggct tgatgtcact caaaaactat ggtgttcaat
     2521 acgtcatgtg gcgccatcct ctaacacctt accgtctgcc gcttaaagag ggtggagatt
     2581 tttactcagt taaaccacaa cctggtggtt taatctggcg cgactggtta ggcctca
//
LOCUS       DADLFA010000285         2591 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000285 DADLFA010000000
VERSION     DADLFA010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.287"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..453)
                     /locus_tag="JQH65_003972"
     CDS             complement(<1..453)
                     /locus_tag="JQH65_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141195.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLITPYRFYHKQWSETLEFWPRKPEPGKDTFQYH"
     gene            complement(580..2541)
                     /locus_tag="JQH65_003973"
     CDS             complement(580..2541)
                     /locus_tag="JQH65_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAY6141196.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
BASE COUNT          605 a          721 c          611 g          654 t
ORIGIN      
        1 atgatattgg aaggtatctt tgccaggttc aggctttcgc ggccagaact ccagcgtttc
       61 agaccattgc ttatgataga atcgataagg tgtgatcaat tgtagcgcct gtaacttctc
      121 gatactgagc ggctcaatac ctttagcctg ataataatgc agttgttctt tttttgcttt
      181 aaaaccggcc cgaacaataa gccccatcat aattaataga taaagaaaag agcatcccgc
      241 ggtaatcagg cctctttcat tcaaaccgtt ggatgttatc gctgcgaaca caaacattac
      301 agcgacaaca catgttaaat aaaaccccca cttacaaagc agcatggcct tattttcttt
      361 aatcatccgt tcaaaattac tattaaatat ttcccagcca ttaaaagaat acttctcgct
      421 cccaggatgg ttttgtaata aaactttttt catcacattc cctgttatta catactttag
      481 aggcgataat tttatccatg caaaaaaata tccaacgaat tttcttgatt cgctggatat
      541 ttcagaatat gactcgaata gcacgaaaga ttcactcgct tacgctatcg ccccgcttcc
      601 gacttcatct gctggcggac tttttttcgc actacgttta cgcggtgcag cctttttctt
      661 atcagcactg ccaccactgc ccggagccac aatgccgcga aactgccgca ccggcgtacg
      721 tttggcttga tcaataagct gatatagcgt ccccaccagc ggctgcataa agtcctgata
      781 gcgacactgc ttttcgctga tttgcgtcag caccgattcc cagtgcgcgg tcatgtccgg
      841 tcgcgtcgcc atttctggca gcgaatggaa taacgctttt ccggcgtcgg tagagtggat
      901 atagcgccct tttttggtca gaaaaccacg cttgaacagc agttcaataa tcccggcacg
      961 ggttgcttct gtccccagac catcggtcgc acgaaggatc tttttcagat ctttgtcctg
     1021 cacaaagcgc gcgatcccgg tcatcgccga aagcagtgtt gcatcggtaa aatggcgcgg
     1081 cggctgggtt tgccgctcta ccacttcacc tttttcacac agcaactcat cgcctttcgc
     1141 caccacaggc aatggcgtac cgtcgttttc ttcatcgcgc tctttgctgc ctaacagcgt
     1201 gcgccagcct gcttcagcaa gaaaacgcgc tttagcgaca aatttgcctt tggcaatgtc
     1261 cagttcgata acacacttgc ggaacaccgc atccgggcag aactgcatca agtactgacg
     1321 ggcaatcagg ttatagacct tcgcttcgtt ctccgtcagg ttgatcgcag aactccgtgc
     1381 ggtcgggatg atggcgtggt gtgcatcgac ctttttgtca tcccaacagc ggttgcgtat
     1441 atctggatct accactggtt gcggcaacag atctggtgca tgaacgctga tggcattcat
     1501 caccgcgtgg cgtccggcaa aatgttcttc tggcaaatag cgacaatcgg aacgcggata
     1561 agtgattagc ttgtgcgttt cgtacagctt ctggcagata tcaagcacgt tctgcgcgct
     1621 cagaccaaag cgttttgccg cttcaatctg caacgccgaa agcgaaaaag gcagcggcgc
     1681 ggattctgat tcccgtttat cgttatagct ggtgacaatt gctggttgac cactaatgcg
     1741 gttaaccaca tgctccgcca gtggacgatg taacaagcgc ccttcttcat cctggtacgg
     1801 ttcacacgct tcgctcggtt gccagatagc ggtaaaccgc tcatcggcag gtgtcacgat
     1861 atgtgctttg acttcaaaga agtctttcgc cacgaagttt tcaatctctt catcgcggcg
     1921 caccaccagc ccgagcacgg gtgtctgcac gcgtcccacg gaaagtacgc cctgataacc
     1981 ggcattgcga ccgagaatgg tatacgcacg ggtcatattg atgccgtaca gccagtcggc
     2041 acgcgctcgc gccagcgcag aaacgcacaa tggcacaaac tcactgttgg accgaagacg
     2101 gtcgatcgcc cgctcaaccg cctgcgggtt caggtcgttt atcaagcaac gctgcacctg
     2161 ctggcgcttt tccggtgcca gttgcagata gtccagcact tcatccacca gcagttgccc
     2221 ttcacgatcc gggtccccgg cgtgaacgat ttcgctggct tcatgcagga agcgtttgat
     2281 gacgttaagt tgtttggtca cggagggtcg gggctgtaat tgccactttt ccgggacaat
     2341 cggcaaatcc gcaagattcc agcgcgcata gcggctgtcg taggcgtctg gctgcgcctg
     2401 ctcaagcagg tgaccgatac accaggtcac cacctgacca ttaccgcact cgataaagcc
     2461 atcgcctttc cggtgcggtt tgggcaggac atcagcaatg gcgcgcgcca gactcggttt
     2521 ttcggcaata aacaaccgca ttgaattaac gaatctcaac catggcacga ccgccgcgcg
     2581 ctggcaccag t
//
LOCUS       DADLFA010000286         2578 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000286 DADLFA010000000
VERSION     DADLFA010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.288"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..2269
                     /locus_tag="JQH65_003974"
     CDS             <1..2269
                     /locus_tag="JQH65_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY6141197.1"
                     /translation="KKKVSQKKITFTANAEQLLQPQFTVEQLRELGIKVDEIPALAEK
                     DDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQIALYRDARGYVSPSRWDDGIPTLFT
                     NYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPWRLRNYSTWTRNDQASSWNTISSYLQ
                     RDIKALKSQLLLGESATSGSIFSSYTFTGVQLASDDNMLPNSQRGFAPTVRGIANSSA
                     IVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSGDLEVTIEESDGTQRRFIQPYSSLPM
                     MQRPRHLKYSATAGRYRADANSDSKEPEFAEATAIYGLNNTFTLYGGLLGSEDYYALG
                     IGIGGTLGALGALSMDINRADTQFDNQHSFHGYQWRTQYIKDIPETNTNIAVSYYRYT
                     NDGYFSFDEANTRNWDYNSRQKSEIQFNISQTIFDGVSLYASGSQQDYWGNNEKNRNI
                     SVGVSGQQWGIGYSLNYQYSRYTDENNDRALSLNLSIPLERWLPRSRVSYQMTSQKDR
                     PTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHNSSLNASYRSPYGTFSAGYSYGNDSS
                     QYNYGVTGGVVIHPHGVTLSQYLGNAFALIDANGASGVRIQNYPGIATDPFGYAVIPY
                     LTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNRGAMVAARFNANIGYRVLVTVSDRNG
                     KPLPFGALASNDDTGQQSIVDEGGILYLSGISSKSQSWTVRWGNQADQQCQFAFNTPD
                     SEPTTSVLQGTAQCH"
     gene            2280..>2578
                     /locus_tag="JQH65_003975"
     CDS             2280..>2578
                     /locus_tag="JQH65_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY6141198.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKL"
BASE COUNT          718 a          624 c          603 g          633 t
ORIGIN      
        1 taaaaagaag gtttcacaga aaaaaattac atttaccgcc aatgcagagc aacttctgca
       61 gccacagttt acggtagaac aactacgtga gctgggtatt aaggtggatg aaatcccggc
      121 gctggctgaa aaagatgacg atagcgtgat caactcgctt gaacaaatca ttcccggtac
      181 agctgctgaa tttgatttca atcatcagcg acttaatttg agcattcccc aaattgcact
      241 gtaccgtgat gcaagaggtt acgtctcccc ttctcgttgg gacgatggta taccaacgct
      301 gtttaccaac tactcgttta caggttctga taaccgttac cgccagggca atcgtagcca
      361 acgacagtac ctgaatatgc aaaatggtgc taattttggc ccctggcgat tacgtaacta
      421 ttctacgtgg acacgcaacg atcaggcgtc aagctggaac actatcagta gttatttaca
      481 acgtgatatc aaggcgttga agtctcagtt gcttctggga gaaagcgcca ccagcggcag
      541 tattttttcc agctacacct ttactggcgt gcaactcgct tccgacgata atatgttgcc
      601 aaacagccag cgcggatttg ccccaacggt acgcggtatc gcaaacagta gtgcaatcgt
      661 gactatcagg caaaatggtt atgtgatcta tcaaagcaac gtgccagcgg gtgcctttga
      721 aattaacgat ctctacccct cttccaacag cggcgattta gaagtcacga ttgaagaaag
      781 tgacggtaca caacgtcgct ttatccagcc ttattcttca ttacccatga tgcagcgacc
      841 taggcatcta aaatatagcg cgaccgctgg acgctatcgc gctgatgcaa acagtgatag
      901 caaggaaccc gaatttgctg aagccacggc aatatatggt ttgaataata cttttacgct
      961 gtatggcggc ctgctcggtt ctgaagatta ttatgcgctg gggatcggta tcggcggcac
     1021 acttggcgca ctgggcgcgt tgtcgatgga tatcaacaga gctgacaccc aattcgataa
     1081 ccagcactct tttcatggct atcaatggcg tacgcagtac atcaaagata tcccggaaac
     1141 caacaccaat atcgctgtta gctactatcg ctataccaac gatggctatt ttagttttga
     1201 tgaagccaat acccgcaatt gggactataa cagtcgccaa aaaagtgaaa ttcaattcaa
     1261 catcagccag acaatatttg atggggtaag tctgtatgcc tccggttcgc agcaagacta
     1321 ttggggcaat aacgagaaaa acaggaatat ctctgttggg gtttccggcc agcaatgggg
     1381 aattggttac agcctgaatt atcaatacag ccgctacact gatgaaaata atgaccgtgc
     1441 actctctttg aatctcagta ttccgttaga acgctggtta ccgcgtagcc gggtttccta
     1501 tcagatgacc agccagaaag atcgcccaac ccaacatgaa atgcgtcttg atggctcact
     1561 gctggatgat ggtcgcctga gctatagcct ggaacaaagt ctggatgacg ataacaacca
     1621 taacagtagc ctgaacgcca gttaccgttc accttatgga accttcagtg ccggatacag
     1681 ttacggtaat gacagcagcc aatacaatta cggcgttacc ggcggcgtgg ttatccatcc
     1741 tcatggcgtg acgctctcgc aatatctggg caacgctttt gcgcttatcg atgctaacgg
     1801 ggcttctggc gtgaggatac aaaactatcc ggggattgct actgatccct ttggctatgc
     1861 agtgattcct tatctcacga cttatcagga aaaccgtctc tcggtagata ctacgcagct
     1921 gcccgataac gtcgatcttg aacaaacaac acagtttgtg gtgcccaaca gaggtgcaat
     1981 ggtagcggcg cgtttcaacg ccaatatcgg ttatcgcgta cttgttacag tcagcgatcg
     2041 caacggtaaa ccgttgccct ttggcgctct tgccagcaac gatgatacgg ggcaacaaag
     2101 tatcgtcgat gagggcggca tactatatct ctctgggata tcgagtaaat cacaaagctg
     2161 gactgtacgc tggggaaatc aggcagatca acaatgtcag tttgctttta atacaccgga
     2221 ttcagaacca acaacctctg tattacaagg cacagcgcag tgccattaag gataaaaaaa
     2281 tgaaaagagc gcctcttata acaggacttt tgttgatatc cacatcctgc gcttatgcct
     2341 cctcaggagg gtgtggagcc gacagcacta gcggtgcgac aaattacagc agtgtggttg
     2401 atgatgttac ggtgaaccag acagataacg tgacaggacg ggagtttacc tctgcaacgc
     2461 taagtagcac taactggcaa tacgcctgtt cctgctctgc gggtaaggca gttaaacttg
     2521 tctatatggt cagccccgta cttaccacca ctggacatca gacaggatat tacaaact
//
LOCUS       DADLFA010000287         2562 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000287 DADLFA010000000
VERSION     DADLFA010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.289"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..657
                     /gene="hexR"
                     /locus_tag="JQH65_003976"
     CDS             <1..657
                     /gene="hexR"
                     /locus_tag="JQH65_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator HexR"
                     /protein_id="HAY6141199.1"
                     /translation="QSLANGTPYVNRNVNEDDSVESYTGKIFESAMATLDHVRHSLDK
                     SAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYSDDIVLQRMSCMNC
                     SDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLAREATLAITLDVPEDT
                     DIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKESRFDKQLLNLSDDR
                     "
     gene            785..2227
                     /locus_tag="JQH65_003977"
     CDS             785..2227
                     /locus_tag="JQH65_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase II"
                     /protein_id="HAY6141200.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(2287..2505)
                     /locus_tag="JQH65_003978"
     CDS             complement(2287..2505)
                     /locus_tag="JQH65_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141201.1"
                     /translation="MEYQADYYITINDSIKTWVQTQYSKDSGLPMAVIGHSVAEEAGM
                     RRLASYLDLHSGYPCIHFTGGCDYDWIE"
BASE COUNT          659 a          627 c          649 g          627 t
ORIGIN      
        1 cagagtctgg cgaatggcac tccctatgtt aatcgcaatg tcaatgaaga tgacagcgtt
       61 gaatcataca cagggaaaat atttgagtcc gcaatggcaa cgcttgatca tgtccgtcat
      121 tcactggata aatctgccat caaccgcgcc gtcgacttgc tcactcaggc aaaaaaaatc
      181 gcctttttcg gattaggctc ttcagccgcc gttgcccacg atgcgatgaa taagttcttt
      241 cgttttaatg ttccggtggt gtactccgat gatatcgtgc tgcaacgcat gagttgtatg
      301 aattgtagcg acggagacgt ggtggtgctg atttctcaca ctggaagaac aaaaaatctg
      361 gtcgagctgg cgcagctggc tcgcgaaaac gacgcaatgg tgattgccct cacctctgcg
      421 ggtaccccgc tcgcccggga agcaacgctg gcaattaccc tcgacgtacc ggaagatact
      481 gacatttata tgcccatggt ttctcgactt gcacagctga ccgtgataga tgtgctggcg
      541 acaggattta ctttgcgacg cggtgcaaaa ttcagagata acttgaagcg ggtcaaggaa
      601 gcgctgaagg aatcgcgttt tgataagcag ttacttaatt taagtgacga tcgctaaaaa
      661 cgactgtcac tgtcctaatc ttatacgaca tccgaatgag attaatttat cgccatcgcg
      721 gcgttatttc attcggattt catgttcaag caacacctgg ttgtttcagt caacggagta
      781 ttacatgtcc agaaggcttc gcagaacaaa aatcgttacc acgttaggcc cagcaacaga
      841 tcgcgataat aatcttgaaa aagttatcgc ggcgggtgcc aacgttgtac gtatgaactt
      901 ttctcacggc tcgcctgaag atcacaaaat gcgcgcggat aaagttcgtg agattgccgc
      961 aaaactgggg cgtcatgtgg ctattctggg tgacctccag gggcccaaaa tccgtgtatc
     1021 cacctttaaa gaaggcaaag ttttcctcaa tattggggat aaattcctgc tcgacgccaa
     1081 cctgggtaaa ggtgaaggcg acaaagaaaa agtcggtatc gactacaaag gcctgcctgc
     1141 tgacgtcgtg cctggtgaca tcctgctgct ggacgatggt cgcgtccagt taaaagtact
     1201 ggaagttcag ggcatgaaag tgttcaccga agtcaccgtc ggtggtcccc tctccaacaa
     1261 taaaggtatc aacaaacttg gcggcggttt gtcggctgaa gcgctgaccg aaaaagacaa
     1321 agcagacatt aagactgcgg cgttgattgg cgtagattac ctggctgtct ccttcccacg
     1381 ctgtggcgaa gatctgaact atgcccgtcg cctggcacgc gatgcaggat gtgatgcgaa
     1441 aattgttgcc aaggttgaac gtgcggaagc cgtttgcagc caggatgcaa tggatgacat
     1501 catcctcgcc tctgacgtgg taatggttgc acgtggcgac ctcggtgtgg aaattggcga
     1561 cccggaactg gtcggcattc agaaagcgtt gatccgtcgt gcgcgtcagc taaaccgagc
     1621 ggtaatcacg gcgacccaga tgatggagtc aatgattact aacccgatgc cgacgcgtgc
     1681 agaagtcatg gacgtagcaa acgccgttct ggatggtact gacgctgtga tgctgtctgc
     1741 agaaactgcc gcagggcagt atccgtcaga aactgttgca gccatggcgc gcgtttgcct
     1801 gggtgcggaa aaaatcccga gcatcaacgt ttctaaacac cgtctggacg ttcagttcga
     1861 caatgtggaa gaagctattg ccatgtcagc aatgtacgca gctaaccacc tgaaaggcgt
     1921 tacggcgatt attaccatga ccgaatcggg tcgtaccgcg ctgatgacct cccgtatcag
     1981 ctctggtcta ccaattttcg ccatgtcgcg ccatgaacgt acgctgaacc tgactgctct
     2041 ctatcgtggc gttacgccgg tgcactttga tagcgctaat gacggcgtag cagctgccag
     2101 cgaagcggtt aatctgctgc gcgataaagg ttacttgatg tctggtgacc tggtgattgt
     2161 cacccagggc gacgtgatga gtaccgtggg ttctactaat accacgcgta ttttaacggt
     2221 agagtaatct cacctgcccc ggccgctgat ggccggggta tttagcaaca cataaattag
     2281 gactacttat tcaatccaat cataatcaca gccccctgta aaatgaatac agggatagcc
     2341 tgaatggaga tctaaatagc ttgcaagacg tcgcataccc gcttcctctg caacactatg
     2401 tccaataact gccatcggta agccactatc tttggagtat tgtgtctgca cccatgtttt
     2461 aattgaatcg ttaatcgtaa tataataatc agcctgatat tccatatatt gtattggatc
     2521 acaaaaacag cctgccccaa tgccaacagt tctgactact ct
//
LOCUS       DADLFA010000288         2468 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000288 DADLFA010000000
VERSION     DADLFA010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.290"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(28..900)
                     /locus_tag="JQH65_003979"
                     /pseudo
     CDS             complement(28..900)
                     /locus_tag="JQH65_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
     gene            complement(914..1054)
                     /locus_tag="JQH65_003980"
     CDS             complement(914..1054)
                     /locus_tag="JQH65_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141202.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            1193..1864
                     /gene="queE"
                     /locus_tag="JQH65_003981"
     CDS             1193..1864
                     /gene="queE"
                     /locus_tag="JQH65_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HAY6141203.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          737 a          482 c          522 g          727 t
ORIGIN      
        1 tccgggatgg cattttttat ggttgagtta ccagcggccg gatgcgccgc cacctccaga
       61 agaaccgcca ccgccgctaa atccgccgcc acttgagcct ccaccggaac cgctgttgtc
      121 tgaatgataa cttttacctg atgctctttt ctgaagtaca cataaacagg taaagaccat
      181 aaatatggtg aaagtaaaaa agaaggaaaa tacagcaatg gaaaaaattt tatcggagaa
      241 caggaataaa tctaaaagat agattgctga aatacagact ccacttttac atgcgcgaca
      301 aaaattactg ccttgatgaa aaaaccagaa agggaagaac atcactgcaa atacccaaaa
      361 aatagcaaag taataatgat cactggaaga cgctgattca ctttctgaag gattagtcgg
      421 atattggtgt tgtgaagtga gttggttatt caaagcgttt atagctagct ctaatccctg
      481 agcaaatttt tgttgtttaa atgcgggtat catgttgcta cggatgatat ctccagccag
      541 agcatcggtt accttttctt ccagcccata acctacctgg atgcggacag tacgatctga
      601 ccaggcaaca atgatcagta tcccatcatt acgtttggca tctcctaaac gccaattgtc
      661 aaaaactctt gtagcatatt gttcaattgt ctcgtctttg gttgtgggaa caattaatac
      721 agcaatttgc acttgggtat tattgaaaat agattctaac ttgtgcgtca gttctttctt
      781 ttcttgtgag tttaagctac tcgtaagatc cgtaacttgt tactgtaatt gtgggacaat
      841 agttggttgg gctgcaacaa aggaacatac gaaagtgaac attagtatga aatagcgcat
      901 tataaatttc tcattaaatc tggataaata aattgacggc ggcatcggta attttagact
      961 cgttatcatt aaaaactaat acagaaaaaa taaatataat tttggtgaat gttttgacat
     1021 gattaaatcc attttcttta ttttcttctg acataatacg ttactccctt taattattaa
     1081 cgatatgttt cgcatttttc tttaaatctg catgtaacag aatgttaata aggcatgatt
     1141 gttgcggcag gatctgcaat aattaacaat tatccacttc acagagaatg ctatgcagta
     1201 cccgattaac gagatgttcc agaccctgca aggtgagggt tactttaccg gcgtccccgc
     1261 catttttatt cgtttacagg gatgcccggt tggctgtgcc tggtgcgaca ccaaacacac
     1321 ctgggaaaag cttgaggatc gggaagtctc ccttttcagc attctggcga agaccaaaga
     1381 gagtgataag tggggggcgg cgagcagtga agatttgctg gctgtcattg gtcggcaggg
     1441 atataccgcg cggcatgtgg tgattacggg tggtgagcct tgcattcatg atttgctgcc
     1501 actgactgac ctgctcgaaa agaacggttt tagctgccag atcgaaacca gtggaactca
     1561 tgaagtacgc tgcacaccga atacctgggt taccgtatcg ccaaagctga acatgcgcgg
     1621 cggctatgac gtgttgtcac aggcactgga gcgagccaac gaaatcaagc atccagtggg
     1681 gcgcgtacgc gatattgaag cactcgatga actattggca acgctgaccg atgataaacc
     1741 gcgagtcatt gcactgcagc cgattagcca aaaggatgat gccacacgtt tgtgcattga
     1801 aacctgcatt gcgcgtaatt ggcgtttgtc gatgcaaaca cataaatatc taaatattgc
     1861 ctgattaaac atttataagc gttataaatg ggtggaacct gtaaagactt ctactcattt
     1921 atattgtttg tcgcctctga aaactcctcc attttaccca tccagggcta atcattagca
     1981 ttatctacaa attctgtggc attaattttt cgctggagtg aaaattattg cggtaaagtt
     2041 tggtagattt taatttgtat agagttattt taaatattta cctttttaat caatgaatta
     2101 agtactcttt aacataatgg atgtgttgtt tgtgtgatac tgtaaagttg gtagattgtt
     2161 actggcttaa aaaatcatta attaataata ggttatgttt acagagttcc ccgcgccagc
     2221 ggggataaac cgagttacaa cactttgagg cgggcaagag ctgggagttt cccgcgccag
     2281 cggggataaa ccaccatata acccgttatc tctttctcaa gtttttatat tagcagtact
     2341 tgtaataagc aacatatcca cgtaacacct catgttcaaa atagttctcc atgccagaga
     2401 ggttcacaat tatcgataca aaaaattaaa tttaatcaaa gtgttatttg tatgattctt
     2461 aaatcgtt
//
LOCUS       DADLFA010000289         2444 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000289 DADLFA010000000
VERSION     DADLFA010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.291"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..753)
                     /locus_tag="JQH65_003982"
     CDS             complement(<1..753)
                     /locus_tag="JQH65_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HAY6141204.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLALLPHAHQQQNPPQEETVVSIAVDPESPA"
     gene            complement(755..1033)
                     /locus_tag="JQH65_003983"
     CDS             complement(755..1033)
                     /locus_tag="JQH65_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141205.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDWYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            974..1159
                     /locus_tag="JQH65_003984"
     CDS             974..1159
                     /locus_tag="JQH65_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141206.1"
                     /translation="MPAGRRGRVIRIVKFGIVRHLFSLLAQQVPVVQADGWIVNQNDQ
                     KKQNPPKRVKCGCVEDA"
     gene            complement(1201..1413)
                     /locus_tag="JQH65_003985"
     CDS             complement(1201..1413)
                     /locus_tag="JQH65_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAY6141207.1"
                     /translation="MLNTCRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVT
                     RKDLCEVRIRTGQTEVAVFTAYEPEE"
     gene            complement(1616..1834)
                     /locus_tag="JQH65_003986"
     CDS             complement(1616..1834)
                     /locus_tag="JQH65_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141208.1"
                     /translation="MFKCYCTLIVGGIMKNALQFLFVAFWLFASCMPIIFTARYMEKI
                     DVLILMFGHINALFLGVFMAVMCIEYWR"
     gene            complement(2008..2343)
                     /locus_tag="JQH65_003987"
     CDS             complement(2008..2343)
                     /locus_tag="JQH65_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141209.1"
                     /translation="MARVEGELFKIARTSLEAEPIAWRYRYVKKGVMDSQGELWVGDW
                     KYVPKKEDCNDRPNYEIQALFTAPPVPVTSEELVKAVHFYEQLKRENPPASGNQINGL
                     TMSVKRPAN"
BASE COUNT          579 a          638 c          572 g          655 t
ORIGIN      
        1 agccggagat tccggatcaa cggcaatgct gaccaccgtc tcttcctgtg gtggattctg
       61 ttgctggtgg gcgtgaggca gcagcgcaag attttttgtg cgctgcttca gtatgctggt
      121 ggcggtctgc tctcccggca cgatgtcgct ttcacggtac attgagcgga ttttttccgc
      181 acgcaacccc agcgaacgac gtaatactgc ttccggtagc gcgtccgcca cctgattgcg
      241 gaccgcccac caggataatt cagccagaga taattcacgc tcctgcgtac cgcttattgc
      301 gtgaccgatg acgtcaatca tccatgctga caggttttga tgagcaagtt gctcgagtga
      361 ttcggatgtc tggtcacgca actggttgtc gcagtgccag cacaacacca ttgcgccggt
      421 accataacgg tgaatgacgg tttcgctgtg atgataatcg ccgtgtggcc actggcagga
      481 tttaatatgg cgcaacagcc agtcagacaa tgcaccagca ccaccagcag cacgaatcac
      541 ccgtgcgtta ctgaaaaacg gcagcaatgt tttgtcttcc actagcggct ggcgaacggc
      601 aggaacgacc ccggacggca gattacgcat gcttttcggt tccggctcca ccagtacccg
      661 ggtattgtgg aataccggca tggattcacg gcctggctta aggaccacca gcccgagttc
      721 cggaaccaga acaggtcgaa gtaatacccg cacattacct ccagatccgt tgctggaatg
      781 tgcaggacgg acgcggtggc cgttcggaat aagggagcct gacgaagatt atccagtgac
      841 gataatcgag actgagggct ttcttaatct cgtatccgtg tctgcggtag cgctgaatca
      901 gccattcagc ctgttcttcg gtgcaggggt cgtgctgata ccagtcagat ttgaatgcat
      961 gagaacgccg cccgtgcctg ctggcaggcg cggcagagtt atccgaattg taaaatttgg
     1021 tatcgtgcgc catctgtttt ctctgctggc gcagcaggtg ccagttgttc aggctgacgg
     1081 atggattgta aaccagaacg accagaaaaa acaaaacccg ccgaagcggg ttaagtgcgg
     1141 gtgcgttgag gatgcctgac acatcagcgg tggcgaggga tttctccccc gccgggtctc
     1201 ttactcctca ggttcgtaag ctgtgaagac agcgacctcc gtctggccgg ttcggattcg
     1261 tacctcgcag aggtctttcc tcgttaccag tgccgtcact atgacggtta aacagatgac
     1321 gataagggcg attaacatcg ccttttgctg cttcatagcc tgcttctcct tgcctttcgg
     1381 cacgtaagag gctaacctac atgtgttcag catggattga gcctcagatt aatgttaagc
     1441 gtcttgcagg acgcgtagtg ttaactgggg cttttctcta tctgcctttt ggtgttcatg
     1501 cctgagacag atagcctcaa gcacccgcag tcattctact taactaagat ttccctgcaa
     1561 accgtttttg tccggcacag taaatatcca actaaaccaa tagcgttcgc tgtatttacc
     1621 gccagtattc aatgcacatg accgccatga acacccctaa aaaaagggca tttatatgtc
     1681 caaacattaa tatcaaaaca tcaatttttt ccatatacct tgctgtgaag atgatgggca
     1741 tacatgatgc gaacaaccag aacgcaacaa acaaaaactg caatgcgttt ttcattattc
     1801 cccctacaat caatgtgcaa taacatttaa acacacctca atttggccgg acatataaat
     1861 atctaaacca gaaaaaatca cttacatagc gttacaaact ctttagtcta aatactcatc
     1921 gtaaaacatt ccccatactt atcagtccgt tccgcgccag gtagcttatt gccttatctg
     1981 gcaacctata atcaggtttc cgctttttca gttggctggt cgtttaaccg acatagttaa
     2041 cccattaatc tggttgccgg atgctggtgg attttcgcgt tttagttgtt cataaaagtg
     2101 cacagcttta accagttctt ctgatgtaac cgggactggt ggggcagtga ataaggcctg
     2161 aatttcatag ttcggcctgt cgttacaatc ctcttttttc ggtacatatt tccagtcacc
     2221 aacccacaac tccccctgag agtccataac accttttttc acgtagcgat atcgccacgc
     2281 tatcggctct gcttccagcg atgtccgtgc aattttgaat aactcgccct ctactcgcgc
     2341 catccctgaa ttggggtggc atttcgtaat cgctattttt aatttggctt cttcgattaa
     2401 ttgttctttt gttaattcag tcattttcat taccgccctt tcag
//
LOCUS       DADLFA010000290         2419 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000290 DADLFA010000000
VERSION     DADLFA010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.292"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(28..2217)
                     /locus_tag="JQH65_003988"
                     /pseudo
     CDS             complement(28..2217)
                     /locus_tag="JQH65_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
BASE COUNT          702 a          627 c          558 g          532 t
ORIGIN      
        1 gataattccc cggttttttt acgcctgtta atcagctaat ggctgcgggc gaccaatcaa
       61 atacccttgc agatattgca ccccgagctt atgcaatagc gcctgctgct gttgcgtctc
      121 gacaaactcc gcgaccacac tcaatgactt cgctttcgcc agatcggtaa ttgatctcac
      181 aatcatcgca tccagcgtgt tcgtgacaat atctttcaca aagacgccat caattttgat
      241 gatatcagcc tgcaaacgct ttaaccgttc gtagttggca tagccggtgc caaagtcatc
      301 aatcgcaatc cggaaaccaa acttatgcag ctgctcgatg ttgtacatgc tgctttctgc
      361 gttagaaaac gcctgctcct cggtgatctc aagaatgacc gcctgcgggg agatgtgata
      421 acgcttaaac agacgaataa tccgcccggc aatattcttt tgcagcagcg tgagcggcat
      481 taaattgact gaaaagcgcg gtcctttttt gtcgcaaggg tgtgttgcca gccacttcaa
      541 caaggattcc agcacttgca aatcaaaacg cgcgctaagg ttaaactgag caataagggg
      601 cagaaactta tccggggtca taatgccgcc gtcatatttc agtcgcgcga ggatctcatc
      661 ataaccttca ccctctttgt tgcgaattgg ctgggcgtag agcagcaaat caccctgatc
      721 taacgccgtg cgaatggtat tcagcaatag cacctgttta gtggtctgcc cggaaaccat
      781 ctcctctctg ctatccagcg ccagcacatg atgatgtgcg caggattgct ccgccagcca
      841 gcttaactgc cccaacaagg gttgcagggt ttcctgattt ccatcaaaac gcccccaggc
      901 agcaccatag cccatatcca gcccggtatt gttccagtga atttgccgac tattcaggat
      961 gttaaccata tgctggagtc gcccttccgt ttccggcccg ctcagcacta acagcagttc
     1021 actacccggc aattgataca acttttcgtt ttcctgcatc aacggcagca gcgtacggca
     1081 aattgagcgg atacagtgaa cgcgcatcat taagccgtaa tgacgactca taaactcaag
     1141 attatcaatg cgcaggcagc aaaaactctt gcccgcctct tgctccggcg cttgttccaa
     1201 cgcacggaag ttgggcagga gcgttaacgg atcggtcagc gcctgcaaat gccagcggcg
     1261 attaagccat tcactgcgat gataaatgcg caccatatag agcaggcaaa cgctaaagga
     1321 aatcagcacc gcaagaataa atgccagcga atattcggtt tccacccctt gcaaaaagtt
     1381 ctggttgtaa ttcagaaggc aaagcgttga aacagcccag gtgagattta aaaacggata
     1441 gcgaagctta ccgaccccga gggtaaagat gatgaagaaa acgggtacca ggtaaccggc
     1501 aataaagtcg ttttcataag gtgtgcacaa cagaagtaga agcacgctta acgctgccag
     1561 ccagcttaag gtaaatgcgc gtttctcttt gcccaacgac ggcgcgatat ccctgcgcca
     1621 caatatctgc gcaaagtggg gacttacaat catgcgagtg agatagtaga aaagcatgtt
     1681 gtaaatcagc acagcggtga aaaggcttag caaatcaacg accgtgaaaa tggcatccgc
     1741 atcgccgaaa aaggtggata tctttagcgg gaaatcaaag aagctcccca caagatacat
     1801 gctgcatttg atgccgatcg gcgtcaccaa accaagccag aacagacgtt gccagacata
     1861 tcggctggtc aatccataac gccaacgcgt ccccagctgc cagcgtaata ttgcgcaggc
     1921 acacaacacg acaaacgtct ggctaccgag catgaccgca gtctataaaa aagagagatg
     1981 aaagttccac agattggtgc aaaacatccc gcataaaacg ggaacgacac ctcgccagcc
     2041 aaagataaac aagattgaca gcattacgca cagtggcatc cacgccagaa agatataact
     2101 ggaatggata atggcaagtg gcgaaataaa acgggatagc tgaataagta ccacggtgag
     2161 cgtaaacgct agtgtgaata tcttgatatt ttttatcagg ttatgctcca caaacatgag
     2221 tacactactc tcactgatcg gagttttcag gcacacaaat aataagttta ttcgcatcgc
     2281 gaagcaaaca gggggccagc ctgttgccga tttttcatcc aatgatgctt tcaccaggcg
     2341 gcactaaccc ggacgtagac tgtattacaa aagcggcaaa aagcagagac aaaaaacccc
     2401 cgctttgcag cgagggttg
//
LOCUS       DADLFA010000291         2411 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000291 DADLFA010000000
VERSION     DADLFA010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.293"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..447
                     /gene="yeeE"
                     /locus_tag="JQH65_003989"
     CDS             <1..447
                     /gene="yeeE"
                     /locus_tag="JQH65_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HAY6141210.1"
                     /translation="VLIGLIALLAWPLSEATGRMFGLGITSPTANILQFLVAGDVKYI
                     NWGVFLVLGIFVGSFIAAKASREFRVRAADAQTTLRSGLGGVLMGFGASIAGGCSIGN
                     GLVMTAMMTWQGWIGLVFMILGVWTASWLVYVRPQRKARLATAAAN"
     gene            461..688
                     /locus_tag="JQH65_003990"
     CDS             461..688
                     /locus_tag="JQH65_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HAY6141211.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(731..2155)
                     /gene="sbcB"
                     /locus_tag="JQH65_003991"
     CDS             complement(731..2155)
                     /gene="sbcB"
                     /locus_tag="JQH65_003991"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HAY6141212.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIGE
                     PEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMPPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSD
                     TLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPTDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
BASE COUNT          581 a          575 c          621 g          634 t
ORIGIN      
        1 gtactcatcg gtttgattgc gcttttagcc tggcccctga gtgaagcaac cggacgcatg
       61 tttgggttag gaatcacttc cccaacggcc aatattctgc aatttctggt cgcgggtgac
      121 gtgaaataca ttaactgggg cgttttcctg gtgttaggga tcttcgtggg gtcatttatt
      181 gcagccaaag cgagccgtga gttccgcgtt cgcgcagctg atgcacaaac aacattacgt
      241 agcgggttag gtggtgtact gatgggcttc ggtgccagta ttgcaggtgg ttgctctatc
      301 ggtaatggac tggttatgac tgcaatgatg acctggcagg gctggattgg ccttgtattt
      361 atgattctcg gagtctggac tgcatcctgg cttgtgtatg ttcgaccgca gcgtaaggcg
      421 cgactggcga ctgctgcggc aaattaagta aggtgtaaag atggcgatta aaaagctgga
      481 tgtggtgacg caggtctgtc cgtttccact catagaagca aaagcagcac tggcagaaat
      541 ggccagcggc gatgagttgg taattgagtt tgattgcacc caggcaactg aagcaatccc
      601 acagtgggcg gcagaagaag gacatgccat caccgattat cagcagattg gcgatgcagc
      661 gtggagcatc accgttcaaa aagcctgact caacattgtc ctccgccgta ccagcggcgg
      721 aggcttcaaa ttagacgatc tcttccgcgt actgccaaag tgcttttaac agcgccactt
      781 tttctttgtc atcggcatat tgttgtacca gcatctgcaa ttcatcagca taaccctgca
      841 aaaactctgg cgtgaatacc tggcggcggt gctccagcca gcgttgctgc tcggcataat
      901 ccagcgtccc cgggaagttg cgtgcccgat aattgaacaa cagcttttca atccgtttat
      961 caacaaaagt gatatccagt gccggtaaat tacgtggctc ggtttccagc acaattttca
     1021 ttgctgcacg atctgcgtca ctgaaaaagc cgttataaag ctgtgcatcc acgttatctg
     1081 taggcgtaaa cggttcggct tccgcgaata tcgccaccac tttttcacgc acttgcggat
     1141 tttcacgcag aattttcagg ttatcgaggc aatgctgacg attaattccc agtcggtcgg
     1201 catcttccgg gcgtagcgta ttcgcctggg ccagcaccgg acatttattg atgtgcacca
     1261 gcttaaccgg aacggcggcg ttatcgccaa gatcggtttt tgcggtatat aaacgctcgc
     1321 gcaatgtgtc gctatccagt tccagtaatg gcgaaatgtc tcctgccaaa tccaccataa
     1381 ttacagcatt gcgattttca ggatgccacg ccagcggtgc cacccagctg gtattgccgc
     1441 gccatgcccc aaacattccg gaaacgtgaa ccaggggggg catctgcgga acatcaatca
     1501 acgccatcag tttgtgttta ttacgatggg taaagagata atcaaacagg cgtggctgac
     1561 gcgtttttac cagtttcgcc atcgcaatag tggcgtacac atcagccatc gcatcgtggg
     1621 cgttgctatg ttcaatacca ttcgctttgg ttaaatgctc aaggcgaaag ctcggtagac
     1681 cgtcatcatt ttcaggccag tttattcctt ccgggcgcag ggcataacag gcacgcataa
     1741 catccaacaa atcccagcgc gagttatcat gctgccagct ccaggcgtaa ggatcgtaga
     1801 aattacgata aaaaacgttg cgtgtgactt cgtcgtcgaa acgcacattg ttgtagccca
     1861 gaatacaggt cttcggtacg gtaaaaagcg agtgaatacg ggcggcaaac gcggcttcgt
     1921 tttctccttt cgcccgtgct tcctgcgggg taataccggt aattaatact gctccaggct
     1981 ggggtaaata gtcatccgcg ggcttgcagt aaaagacttc gggttcgccg atgacattga
     2041 attcgctatc ggtgcgaatg gctgcgaact gtgcagggcg atctaacgcg gggtgcgtgc
     2101 caaaggtttc gtaatcgtga aagaggaagg taggttgttt atctgtgtct gtcattgtct
     2161 agcattgtcc actgtagtta attaactgtc tgttataact cgaaaaacaa ccattttttc
     2221 acctgagtat gtctacgaac gtcaaatata gttgtttact gtgcgctgtt gtccatgtga
     2281 tattgtgtac cagatgtgta ccaaaccatt atttctgagc gtaccaaata ttatttatgg
     2341 ctataagcga cacaaaactg cgtagtattt atggtaaacc atattcgggc ccacaagaag
     2401 tggctgatgc c
//
LOCUS       DADLFA010000292         2407 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000292 DADLFA010000000
VERSION     DADLFA010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.294"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            128..1264
                     /gene="potA"
                     /locus_tag="JQH65_003992"
     CDS             128..1264
                     /gene="potA"
                     /locus_tag="JQH65_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HAY6141213.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            1248..2111
                     /gene="potB"
                     /locus_tag="JQH65_003993"
     CDS             1248..2111
                     /gene="potB"
                     /locus_tag="JQH65_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HAY6141214.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVSELDD"
     gene            complement(2343..>2407)
                     /locus_tag="JQH65_003994"
     CDS             complement(2343..>2407)
                     /locus_tag="JQH65_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY6141215.1"
                     /translation="LNRVDTSTAPDIEWPTSPAE"
BASE COUNT          573 a          590 c          608 g          636 t
ORIGIN      
        1 ctaagccagt aattcacaac aaagattggc gattcaagcg gtttgccgta gaattaccgc
       61 cctcattaag agtcaccaag gtggttaacc acaaaccccg catcggtaag ccatccgttg
      121 cgtttacatg ggacagagta aaaaattgaa taaacaaccg agttcgcttt caccgctggt
      181 gcaattggcg ggaattcgca aatgctttga tggtaaagag gtcattcccc agctggatct
      241 gactatcaac aatggcgagt tcctcacgct gcttggccct tctggctgcg gtaaaacaac
      301 cgttcttcgc ctgattgcag gtctggaaac tgttgattcc ggacgcatca tgctggataa
      361 cgaggacatc acccacgttc cggcggaaaa ccgctatgtg aacactgttt tccaaagcta
      421 cgcacttttc ccccacatga ccgtgttcga aaatgtggcc tttgggttgc gcatgcaaaa
      481 aacccccgct gctgaaatta cgccccgcgt gatggaagcc ctgcggatgg tgcaactgga
      541 aaccttcgct caacgtaaac cgcatcagct ctctggtggt caacagcaac gcgtcgccat
      601 tgctcgcgcg gtggttaaca agcctcgtct gttgttgctg gatgagtcgc tctcagcgct
      661 ggattacaaa ctgcgtaagc aaatgcagaa cgagctgaaa gcgttacagc gtaagcttgg
      721 cattacattc gtcttcgtga ctcacgacca ggaagaagca ctcaccatgt cagacaggat
      781 tgtggtgatg cgcgagggtc gcattgagca agacggcacg ccgcgtgaaa tctacgaaga
      841 gccgaaaaac ctgtttgttg ccggcttcat tggcgaaatt aatatgttta acgccacggt
      901 gatcgagcga ctcgacgagc agcgcgtacg cgccaacgtt gaaggccgcg aatgtaatat
      961 ctacgttaac ttcgccgttg aaccggggca aaaactgcat gttctgctgc gcccggaaga
     1021 cttacgtgtt gaagagatta acgacgacaa ccacgctgaa gggctgattg gttacgttcg
     1081 tgagcgtaac tacaaaggca tgacgctgga gtcggttgtt gaactggaaa atggcaagat
     1141 ggtgatggtc agcgaattct tcaatgaaga cgatcctgac tttgaccact ctctcgacca
     1201 aaaaatggcc attaattggg tagaaagctg ggaggtcgta ctggctgatg aagaacacaa
     1261 gtaagttcca gaatgtagtg attgtcacta ttgtcggttg gcttgtgttg tttgtctttc
     1321 tgcccaacct gatgatcatt ggcaccagct ttttgacccg cgacgacgcc agtttcgtca
     1381 aaatggtctt tacgctggat aactacacgc gtctgctcga tccgctctat tttgaagtgc
     1441 tattgcactc gctgaatatg gcgctgatcg ccaccctcgc ctgcctggtg ctgggctatc
     1501 catttgcctg gtttctggcg aagctgccac acaaggtgcg tccgctgctg ctgtttctgc
     1561 tgattgttcc gttctggacc aactcattga ttcgtatcta cgggctgaaa attttcctca
     1621 gcaccaaagg ctatctcaac gagtttttgc tctggctggg cgttatcgac acaccaatcc
     1681 gtatcatgtt cacgcccagt gcggtgatta tcggtctggt ttacattctg ctgccgttta
     1741 tggtgatgcc gctgtactcc agtatcgaaa aactggataa gccgttgctt gaggcggcgc
     1801 gcgatctcgg tgccagcaag ttacagactt ttatccgcat cattattcca ctgacgatgc
     1861 cggggattat tgccggatgt ctgctggtga tgctgccagc gatgggcctg ttctatgtgt
     1921 ccgacctgat gggcggtgcg aaaaacctgc tgatcggtaa cgtcatcaag gtacagttcc
     1981 tcaatattcg tgactggccg tttggtgcag ccaccagcat tacgctgact atcgtaatgg
     2041 gcctgatgtt gctggtttac tggcgcgctt ctcgtttgct gaataagaag gtgagcgaat
     2101 tagatgatta atcatttata ttcaatgcat taagtttgtt tttgagtggt tttgccgttt
     2161 aaggtaacgg cgttatagat cctatcaaaa tcaatcaatt ccattctggt tttggcgaaa
     2221 gatttttcga gagcttcggt ctttaactaa aactgattgc gtataacccc aatacccctc
     2281 cccgactatc tgaatggtcg gggaggcact tcacgcacaa aatatcatcc ctgcgccgtt
     2341 gattactctg caggtgaagt cggccactca atatcaggtg cagttgatgt atcaacacgg
     2401 ttcagca
//
LOCUS       DADLFA010000293         2369 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000293 DADLFA010000000
VERSION     DADLFA010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.295"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..73)
                     /gene="csgC"
                     /locus_tag="JQH65_003995"
     CDS             complement(<1..73)
                     /gene="csgC"
                     /locus_tag="JQH65_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="involved in autoagglutination of curliated cells;
                     not involved in production of curli fibers; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HAY6141216.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDV"
     gene            complement(132..587)
                     /locus_tag="JQH65_003996"
     CDS             complement(132..587)
                     /locus_tag="JQH65_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major curlin subunit CsgA"
                     /protein_id="HAY6141217.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(628..819)
                     /gene="csgB"
                     /locus_tag="JQH65_003997"
                     /pseudo
     CDS             complement(628..819)
                     /gene="csgB"
                     /locus_tag="JQH65_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="CsgB; functions as a nucleator in the assembly of
                     curli (coiled surface structures) on the cell surface;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
     gene            1598..2248
                     /gene="csgD"
                     /locus_tag="JQH65_003998"
     CDS             1598..2248
                     /gene="csgD"
                     /locus_tag="JQH65_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HAY6141218.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYSMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            2253..>2369
                     /locus_tag="JQH65_003999"
     CDS             2253..>2369
                     /locus_tag="JQH65_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly protein CsgE"
                     /protein_id="HAY6141219.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSI"
BASE COUNT          675 a          512 c          475 g          707 t
ORIGIN      
        1 acacatcccc ttgctgggtc gtattaaagg ttatctgact ggaaagtgcc gcaaggagta
       61 ataacgcatt catattcttc tcccgaaaaa aaacagggct tgcgccctgt ttctgtaata
      121 caaatgatgt attagtactg atgagcggtc gcgttgttac caaagccaac ctgagtcacg
      181 ttgacggagg agttagatgc agtctggtca actgcagcac cgttgccacc accgaactgt
      241 ttaaccgtca tttcagaatt tttgccgttc cactgatcaa gagtagcgct attaccgaag
      301 ccacgttggg tcagatcgat tgagctgtca tctgagccct gaccaacatc tgcaccatta
      361 ccgccgccat gctgggtaat agtcaagtca gagttacggg catcagtttg cagagcaagt
      421 gcagagttac cgccaccgta ctggtaaatg ttcagctcag aatttgggcc gctattatta
      481 ccgccaccac cgtggttacc gccgccgccg tactgaggaa caacacctgc cagagcgcta
      541 ccggagaata cgattgctgc aattgctgct acttttaaaa gtttcatgta aaacccccat
      601 cggattgatt taaaagtcga atggaaatta acgttgtgtc acgcgaatag ccatttgcga
      661 ctgtctctgc actacaattg ccgttttttg agtaccatac tgtgtaatat ttgctttatt
      721 accagaacct ttctggataa tcatcgcagt attaccataa gcaccttgcg aaatactggc
      781 atcgttggca ctgcccgcct gatcaatata tgcaaggtta ttagactggc cccctgaatc
      841 tccagacaac cagtatcact tatttaagtg atactggttg tctggagatt cagggggcca
      901 gtctacgcaa cctgtatttt gttaacgctg cgttatgatg gaaagtatgt ctgcggaaat
      961 atttttaata actcacccgc gcgttgtatt tttctttttt tgttcatacc aaagtattaa
     1021 aacccacgta aatacgctgt tatctacgca aaaaatattt tgttttcttt taaatctccg
     1081 ttttccgcta tcaaaaatca ccagacagtc atttttcttg cccgtcgctg attgctgcga
     1141 tatgtcttgc gcacaagccg tgacaaacct cgtctttttg gagtaaaaat ttgtccacgg
     1201 aggtgcggag aaaaaacaag aacgttttac atgacgaaag gactacaccg aaatattttt
     1261 tatatgtatt attagtaagt tatcaccatt tgtatgattt tttaaaattg tgcaataaaa
     1321 accaaatgta caacttttct atcatttcta aacttaataa aaccttaagg ttaatatttt
     1381 agtataacaa gttacattta gttacatgtt taacacttga tttaagtttt gtaatggcta
     1441 gattgaaatc agatgtaatc cattagtttt atattttacc catttagggc tgatttatta
     1501 ctacacacag cagtgcaaca tctgtcagta cttctggtgc ttctatttta gaggcagctg
     1561 tcaggtgtgc gatcaataaa aaaagcgggg tttcatcatg tttaatgaag tccatagtat
     1621 tcatggtcat acattattgt tgatcactaa accttctttg caggcgacag ctctcttgca
     1681 gcaccttaaa caatcgctgg caattacagg aaaattacat aatattcaac gttctctgga
     1741 cgatatctct tcaggctcta ttattcttct ggatatgatg gaagcagata aaaagcttat
     1801 ccattattgg caggatactt tgagcaggaa aaacaacaat atcaaaatat tgttgctaaa
     1861 tacgcctgaa gattacccgt accgcgacat tgaaaactgg cctcatatca acggcgtttt
     1921 ttattccatg gaggatcaag aacgtgttgt caatgggttg caaggcgtcc tgcgcggcga
     1981 atgctacttt acgcaaaagc ttgccagcta cttgattacg cattcaggta actatcgtta
     2041 taacagcacg gaatcagccc tccttactca tcgggaaaaa gagatcctga ataagctgcg
     2101 tatcggcgcg tctaataacg agatcgctcg ttcgttgttc atcagcgaaa atacggttaa
     2161 aacgcatctt tataatcttt tcaagaagat agccgtaaaa aaccggacac aagcggtttc
     2221 ctgggcaaac gataacctca ggcgataaag ccatgaaacg ttatttacgc tggattgtgg
     2281 cggcagaatt tctgttcgcc gcagggaatc ttcacgccgt tgaggtagaa gtcccgggat
     2341 tgctaactga ccatactgtt tcatctatt
//
LOCUS       DADLFA010000294         2282 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000294 DADLFA010000000
VERSION     DADLFA010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.296"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..184
                     /locus_tag="JQH65_004000"
     CDS             <1..184
                     /locus_tag="JQH65_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HAY6141220.1"
                     /translation="IYQPVYSPWVNHVERLWQALHDTITRNHQCRSMWQLLKKVRHFM
                     ETVSPFPGGKHGLAKV"
     gene            complement(204..455)
                     /locus_tag="JQH65_004001"
     CDS             complement(204..455)
                     /locus_tag="JQH65_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141221.1"
                     /translation="MPGTTTAMSINFIGITARTMNSNGSHGKPQIPVDYQKLLSIEDI
                     TFCRNRWGNIGENALRRVAVGKKLSFFGSDRGGENAAII"
     gene            378..1068
                     /locus_tag="JQH65_004002"
                     /pseudo
     CDS             378..1068
                     /locus_tag="JQH65_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1085..1264)
                     /locus_tag="JQH65_004003"
                     /pseudo
     CDS             complement(1085..1264)
                     /locus_tag="JQH65_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1594..>2282
                     /gene="ipaH7.8"
                     /locus_tag="JQH65_004004"
     CDS             1594..>2282
                     /gene="ipaH7.8"
                     /locus_tag="JQH65_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HAY6141222.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRI"
BASE COUNT          676 a          514 c          429 g          663 t
ORIGIN      
        1 catttatcag ccggtttact cgccatgggt gaatcatgtt gaacggctat ggcaggcact
       61 tcacgacaca ataacgcgta atcatcagtg ccgctcaatg tggcaactgt tgaaaaaagt
      121 tcgccatttt atggaaaccg tcagcccatt ccccggaggc aaacatgggc tggcaaaagt
      181 gtagcggtat taagcgcagc tatttagatt attgccgcat tttctccacc acgatcagag
      241 ccgaaaaaag ataatttttt tccaactgcc acccggcgta atgcgttttc accaatgtta
      301 ccccacctat tgcggcaaaa ggtaatgtct tcaatcgata gaagtttctg gtaatctact
      361 ggtatttgag gttttccatg actaccgtta ctgttcattg tccgcgctgt aattccgatg
      421 aagtttatcg acatggccgt agttgttccc ggcatgagcg tttccgttgt cgctcatgta
      481 aacgtgtttt ccagctcact tactcttatg aagccagaaa actcggcgtt aaggagcaga
      541 tcgttgaaat ggcacataac ggcgcagggg gctgttatac cgccagaaca ctgaaaatcg
      601 gcatcaatac cgtcatacgg acattaaaaa gctctcgccc cggcggataa catctgcgcc
      661 agtcgctcac gctgatgttg cccttatctg tgaacttgat gagcagtggc gcttcgttgg
      721 cagcaaggcg caacaacact ggccctggta tgcgtacaac actaaaactg gcggtgtgtt
      781 ggcttacact tgcgggccac ggaatgatga gacctgtcgg gaactgttag ccctgttgac
      841 tccgttctgt atcggcatgg tgaccagcga tgactggggc agctacgcca gagaagtgcc
      901 ggaggagaaa cacctgaccg gcaaaatctt cacgcaacgt atgaacgtaa caacctgacc
      961 ctccgccccc gcatcaagcg gctggcacgt aaaacaatct gcttctcgcg ttccgtggaa
     1021 atccacgaaa aagtcatcgg ttccttcatt gaaaagcaca tattctaatg attggaagta
     1081 ctacccggca aaactcattc agtgcattcc agtgattcag gatatagttg aacgccttta
     1141 ccatttcagc atggtttgac aaagttttcc tctggagctt tatccagtca tacaaggact
     1201 tcattcgcgg cacacatttc tcctgcctta ccgcaagccg tttttctgcc ggcctccctc
     1261 gtattaaatg tgtatcgttt ttttacagta attttaattt atcattctat aataggaata
     1321 aatgagcttg gctaaaatat gatataaaac aaatatactt actcggatta catttccttg
     1381 ctcactggaa tgttgaattc agtgttatta ttgactgcag tcacaataaa aaaaagaatt
     1441 ccatagaaaa cctcaacaaa taaattacaa ctaacttctg ttatgtgtaa aatggaaact
     1501 attaaaactt aatatcgaaa atggtaagtg aaatttgtat aaatatacaa ttttaaatat
     1561 ttattctcac aaatataagg ttgccctagc attatgttct ctgtaaataa tacacactca
     1621 tcagtttctt gctccccctc tattaactca aactcaacca gtaatgaaca ttatctgaga
     1681 atcctgactg aatgggaaaa gaactcttct cccggggaag agcgaggcat tgcttttaac
     1741 agactctccc agtgctttca gaatcaagaa gcagtattaa atttatcaga cctaaatttg
     1801 acgtctcttc ccgaattacc aaagcatatt tctgctttga ttgtagaaaa taataaatta
     1861 acatcattgc caaagctgcc tgcatttctc aaagaactta atgctgataa taacaggctt
     1921 tctgtgatac cagaacttcc tgagtcatta acaactttaa gtgttcgttc taatcaactg
     1981 gaaaaccttc ctgttttgcc aaaccattta acatcattat ttgttgaaaa taacaggcta
     2041 tataatttac cggctcttcc cgaaaaattg aaatttttac atgtttatta taacaggctg
     2101 acaacattac ccgacttacc ggataaactg gaaattctct gtgctcagcg caataatctg
     2161 gttacttttc ctcaattttc tgatagaaac aatatcagac aaaaggaata ttattttcat
     2221 tttaatcaga taaccactct tccggagagt ttttcacaat tagattcaag ttacaggatt
     2281 aa
//
LOCUS       DADLFA010000295         2277 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000295 DADLFA010000000
VERSION     DADLFA010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.297"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..350)
                     /locus_tag="JQH65_004005"
     CDS             complement(<1..350)
                     /locus_tag="JQH65_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inhibitor of g-type lysozyme"
                     /protein_id="HAY6141223.1"
                     /translation="MKIKSISKAVLLFALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRN"
     gene            592..885
                     /locus_tag="JQH65_004006"
     CDS             592..885
                     /locus_tag="JQH65_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141224.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            957..1616
                     /locus_tag="JQH65_004007"
     CDS             957..1616
                     /locus_tag="JQH65_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HAY6141225.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1693..2154
                     /locus_tag="JQH65_004008"
     CDS             1693..2154
                     /locus_tag="JQH65_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415699.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HAY6141226.1"
                     /translation="MAEHLMNDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDED
                     TDEIYFTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLA
                     EGKDLPVWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
BASE COUNT          592 a          491 c          588 g          606 t
ORIGIN      
        1 tcgtttcggg tttgcagcac tcgcagctca tatttgccag aagcagggag tgagtattgt
       61 ccgtgactat cgagttctgg ggaatatctg gataaatcaa cagaatcatc gattcctggc
      121 ccgaagaggt aagtatcagc gccttcatta gagatactca catgtacttt ctgacctttt
      181 ttggcataga aggtatatgt atcgtaatcg tatcccttta tttcgccgga gtactgagcg
      241 ctgctatggc cttttctgaa ctcaacattg acattcttac ccgcagcaaa gctcgtggag
      301 gttagcagtg caaacagcaa tacagcctta ctgatgctct tgattttcat tggtagtctc
      361 ctgttcagaa tgagtacgat gaactgttat aatataacaa tccctaacgg gagaattcca
      421 gtggcttttc tgcgttcggt aaaatcccgg cgcggcattt tttgccgggg ggattactta
      481 taaaggggca tggactcaac aaaccgcaga aatctacagc tggctggctt gtggcgcggg
      541 tttcattgat gttagcttat gccaaaacct ggcatcttaa agagtaaaag tatgttttgt
      601 gtgatttatc gaagcagcaa gcgtgaccag acctatttat atgtcgaaaa aaaagacgat
      661 ttttcgcgtg ttcctgagga actgatgaaa ggttttggtc agcctcagtt agcgatgatt
      721 ctgccgttgg atgggcggaa gaaactggtc aatgctgata ttgaaaaggt taaactggca
      781 ttaaccgagc aaggttacta tttgcagtta ccgccaccac ccgaagattt gctgaagcaa
      841 catctttccg tcatggggca gaaaacagac gacacgaaca aataaccgat atccggcggt
      901 ggcattatct ttgtcggcgc gggttttcat atccacgata aggtgagggg aacgttatgt
      961 atcaacatca caactggcaa ggtgcgctgc tggattatcc ggtgagtaaa gtagtctgtg
     1021 ttggcagtaa ctatgccaaa catattaaag agatgggcag cgcagtgccc gaagagccag
     1081 tgctgtttat taaaccagaa acggcactgt gcgatctgcg gcagccattg gcgatcccat
     1141 ccgatttcgg ttcagttcat catgaagtcg aactggcggt gttgattggt gcgacgctgc
     1201 gtcaggcaac ggaagagcat gtccgcaaag ccattgccgg ttacggcgtg gcgcttgatc
     1261 tgacattgcg tgatgttcag ggaaaaatga agaaagccgg gcagccgtgg gaaaaggcta
     1321 aagcgtttga taactcttgt ccgctttccg ggtttatccc cgcggcggaa tttaccggcg
     1381 atccacaaaa tacaacgctg ggcctgagcg taaacggcga acaacgccag caaggtacga
     1441 ctgcggacat gatccataaa atcgttccgt tgatcgctta tatgagcaag ttttttaccc
     1501 tcaaggccgg tgacgttgtg ttgacaggca cgcctgatgg cgtcggcccg ttgcaaagcg
     1561 gtgatgagct gacagtcact ttcgatgggc attctttgac aactcgcgtt ttgtaatact
     1621 ttttgccgcc tgaaagcggc ggcaaaactt gcatcgctgt gccagactgg ttataaggtg
     1681 cgttttaacg taatggcgga acacctgatg aacgatgtac ctttctggca aagtaaaacc
     1741 ctggacgaaa tgagcgatgc ggaatgggag tcgttgtgtg atggttgcgg tcagtgttgc
     1801 ctgcataaac tgatggatga agacaccgac gaaatctact tcactaacgt cgcctgtcgc
     1861 cagctcaata ttaaaacctg tcaatgtcgg aactacgaac gtcgtttcga gtttgaaccc
     1921 gactgcatta aattaacccg tgaaaatctg ccaacattcg aatggctgcc aatgacctgc
     1981 gcttatcgtt tgctggcgga aggtaaagat ttacctgtgt ggcatccgct acttactggt
     2041 tcgaaagcgg caatgcatgg tgaacgtatc tctgtgcgtc atatcgcagt gaaagaatca
     2101 gaagtgattg actggcagga tcatatctta aataaacctg actgggcgca gtgataaatt
     2161 taatagatgt tgatgggggc tttcttaata tacgtctgga tgctcgccat ttcgtagccc
     2221 tgtttttgaa agcgctctgt cataatgaga gttacccgat accttattat caacagg
//
LOCUS       DADLFA010000296         2260 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000296 DADLFA010000000
VERSION     DADLFA010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.298"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            111..432
                     /locus_tag="JQH65_004009"
                     /pseudo
     CDS             111..432
                     /locus_tag="JQH65_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232281.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(466..672)
                     /locus_tag="JQH65_004010"
     CDS             complement(466..672)
                     /locus_tag="JQH65_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141227.1"
                     /translation="MTVSYKITYEHRDGDQIRMEEVLFHSEHEPTKDEIHDAVRKDTV
                     RFNSPSNGVTAVSGFKVISVVPVP"
     gene            complement(1170..1243)
                     /locus_tag="JQH65_004011"
     tRNA            complement(1170..1243)
                     /locus_tag="JQH65_004011"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1209..1211),aa:Gly,seq:ccc)
     gene            complement(1322..2077)
                     /locus_tag="JQH65_004012"
     CDS             complement(1322..2077)
                     /locus_tag="JQH65_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAY6141228.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKTGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
BASE COUNT          603 a          530 c          491 g          636 t
ORIGIN      
        1 tcatacacta aatcagtaag ttggcagcat taccaatttt gctaagttaa tgcattaatc
       61 taaaaacgct ttaaatcaat acataaccat tatctcttgg gattatttac atgcgcaaaa
      121 gtttaatgct gttacttgct tcaacattga ttatttctgg atgtgcaaca aaaaaacaac
      181 tcgttccaac tggtggaagc aaatcagacg gcacagttag gatgagttat tcttacggta
      241 tgtttgaaag tcctgttgta gatccacaac aaggcatgaa tgcagccaaa gcgcgttgtg
      301 ccgcttaggg gatattcagg agccgagcct tttggtggat tcacttctca atgttcacaa
      361 ccttcttcat ctggttgtat gcaaacactt gtgactatcg aatatcaatg cactggcgat
      421 attaagaagt aataattagg ggcttccagc cccttctata accattcatg gaacagggac
      481 aaccgaaatc actttaaacc ctgaaacagc cgttactcca ttagatggag aattaaatct
      541 cacagtatct tttcttactg catcatgaat ttcatctttt gttggttcat gctctgaatg
      601 gaataaaacc tcttccattc taatttgatc accgtctcta tgctcataag taatcttgta
      661 gctaacagtc atatcaacct catgcagggg aattgtgaga ttattataga caatagaaac
      721 cataaagccc cgttggtgat acagcaacaa gcgggagcgt agcgacttca tcagattgtc
      781 agaaagatca caccttatca ggataaccgt gtacccgttg ggagcgtggc tgaaacgctg
      841 atagagcttg catatggttt gcaaggacca atatcaccat aaacctgttt acgcagccac
      901 aaggccgcac atcgggctta aatgcgtgtg ttcagtctgt cgatgacatt acaatgccag
      961 gatgggcttt atcttcacgt tgtgctatcg cggtgtgaga ggtgcaactg ccgccaacgt
     1021 taggttatac tgtcatctca ccgtgtaact taacgtgaca gttactaagg gcggatcaca
     1081 atggtagaac tcctaagcaa attaaatagg taactgttta agttgctgac agagaagact
     1141 tttttagtct tctccacctg gggagattct ggagcgggcg aagggaatcg aaccctcgta
     1201 tagagcttgg gaagctctcg ttctaccatt gaactacgcc cgcttcgaga tgcgtaaggc
     1261 attataaacc ttacgctctc cttagcaagt gccgcgctgc tgactgctga ttaattcgcc
     1321 atcagcattt tggcttgctg ccctgcggcg gcaagtaacg tagcggatca attgccgttg
     1381 cacggtaacg aatctggaaa tgcaggcgaa cagatgccgc atccgtgctc cccatagtgg
     1441 cgattttttg cccagtcttc acgctttgcc cattatttac cagcatcgtg tcattatggg
     1501 cgtaagccgt aatgtaatct tcactgtgtt taatcatgat gagattaccg tagccacgca
     1561 gctggttgcc cacatacacc acctttcctg cacccgcggc gtaaataggt gtaccccgtg
     1621 gagctgagat atcaatccct ttattgccgc catctgctgt cgaatacggc atgataactt
     1681 tccctgtcgt tggccataac caacaacgtt gccctactgg cggccaggaa gatttcggta
     1741 ccgctgatga cggtgtaacc gatgcggttt tggtcgttga tttggcggtt gatttacgtg
     1801 tactactgct acttttcgcc ccacccagtt ttagtttctg accaacttca atggtgtaag
     1861 ggggggaaat gccgttcagt cgcgccagtt cttttacgct ggttcccgtg gtgcgcgaaa
     1921 tacgatatag cgtatccccc cgtttcacgg tgtaaacgga gccggaatat gttcctgtat
     1981 cggatgattt gctacccgaa cagcccgcca aaagcagtcc aaccgataac aacatcacga
     2041 tacccagaga ttttttattc aggcgtcccg cactcaaaat ctttcctcgc ttaaaaaaca
     2101 agacgcctta tcatagcagc cagcctcttg atacccaatc tcttgcttct ggaaaaacgt
     2161 ggtaatagag aaagaggtta atttcatttg gcaaagggaa gtatcatcag caaaatattc
     2221 gctgtagagg tatcgaactt cctgactttt taccctattt
//
LOCUS       DADLFA010000297         2208 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000297 DADLFA010000000
VERSION     DADLFA010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.299"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(118..1572)
                     /gene="ospC2"
                     /locus_tag="JQH65_004013"
     CDS             complement(118..1572)
                     /gene="ospC2"
                     /locus_tag="JQH65_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HAY6141229.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIF
                     NTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLS
                     TDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNAKSDMALYLLSK
                     FNFTKQDIAEMEKMNNNMYCELYDVEYLLSEDSANYKVLEYFINNGLVDVNKRFQKAN
                     SGDTMLDNAMKSKDSKTIDFLLRNGAVSGKRFGR"
     gene            1997..>2208
                     /locus_tag="JQH65_004014"
     CDS             1997..>2208
                     /locus_tag="JQH65_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY6141230.1"
                     /translation="MARYDLPDEAWTIIKPLLPPEPTTPRAGRPWAEHRKIINGMFWV
                     LCSGAPWRDLPERYGAWKTVYNRFNR"
BASE COUNT          684 a          405 c          366 g          753 t
ORIGIN      
        1 cgttcaccac atgcaatgtg ttgttgcagg ggaacggtct gcaccatccc ctatatcgat
       61 caatataaca aaaattgaca acgtcagatt cagggcacaa cctaaatata ttttaggtca
      121 cctcccaaat cgtttgcctg ataccgctcc atttcttaat aaaaaatcaa ttgtttttga
      181 atctttgctt ttcattgcat tgtcaagcat agtgtcccca ctatttgctt tttgaaatct
      241 tttgtttaca tcaaccaatc cattattaat aaaatattct agcactttat agttggcact
      301 atcttcacta agtaaatact caacatcata cagctcacaa tacatattgt tgttcatttt
      361 ctccatctct gcgatatctt gttttgtaaa attaaattta gatagtaaat aaagtgccat
      421 atcagatttt gcattgccta gagaataagc aagagcttga tgagccattt ttttatcagt
      481 caccttattg ttaatttctt cataattgga tgctttaatg gcatcctcca aagatatatc
      541 tctgagtctt atctttttga agttatctgt gcttagcatt cttggtatat gatactcaag
      601 ctgaaacaca aagtttataa ttctatccag actaacagga tcaatgtttt tattataaca
      661 aaactctcgg aatctttgat ctttactctt tcttataaaa tcaataagat gcaacccaat
      721 tccaagcctc atatctttag tattaaatat tggcacatca gtttttccct gattaccatg
      781 cacatacctg ctaacttcat ccacaacctc tttaaagtta tctaagaaaa atgattggtg
      841 ctcatggtaa aaaacaggtg gaatagcatt atcaaagtgg tcggttaatg tcatatatcc
      901 atatggagag tcatgatcaa tgatatacgc attagcacca aaatccacag tatgatgtgt
      961 tttatttagt gggagtgttt cttgatgatt ggaaatttca accccaaaaa aaacaaaatc
     1021 gtggttagct atttcttcaa tatcctcaga gaatgtgttt ttatatgttt gtatattctc
     1081 agacttaatt ttatcaaagg acttaatatt aagagtatta tttgacaggt tagcgttact
     1141 ttgatgtctg aagttaaaat cagaattaat aataatagag agcaagtgac gttcatccga
     1201 agaaagagga tgagtttttt gtattatttc agaaagatag tgtctggaca ccttatcgag
     1261 gtgctcaagt gaccgaaatg atgaccgtgc tccagatggc gcttcaatag aaagatttaa
     1321 tgatttgaaa ctggtccctt gagagaacat tttactataa ctttgcgctg ctatagactt
     1381 ccttaaaaag cttttcacag tatcagcaca gtgatctaaa tgtttttcac ttatgccaga
     1441 agaggatgag tttgttacgc atgatatgtt tttttgtaat gcttttgtat ctttgttcgg
     1501 atttattttt ccatcaacaa gatcaatatt gttctgaaca ttaatatgat ttactgcttc
     1561 aggtattttc ataaaacatc ccccattatg tgcgttttta tctgcagtta tttagaataa
     1621 tattatctaa cttaatattt tcttgtatgt attataacga acatatttta atatgttgta
     1681 cgattttcac tctgaaatcc cctgagttta atcacacttc ctttactgaa aggaattatt
     1741 gcaacagtat tatttgtttt tggatatata tcttgcgcct tgttatgtag ttaatgctca
     1801 gaatatgata gataaaatag agacgcagaa cagggcatgc tcataacatg atgataacat
     1861 aaaaaattag taacaataaa tgaaagaatc atgggcgctc aacaaaaaga gagctaaata
     1921 gctgcgcgga atagtaggct gtgtccctta atcatctgag ttatagtaac tgtctgttta
     1981 cacaacatat tgaactatgg ctcgctacga ccttcccgat gaggcatgga ccatcatcaa
     2041 gcccctgtta cctcctgaac ccaccacacc acgggccgga cgcccatggg ctgagcaccg
     2101 taaaatcatc aatggcatgt tctgggtgtt atgttccggt gcaccatggc gcgatttacc
     2161 cgaacgatat ggggcatgga aaactgttta taaccgcttt aaccgatg
//
LOCUS       DADLFA010000298         2183 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000298 DADLFA010000000
VERSION     DADLFA010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.300"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            44..1270
                     /gene="dsdA"
                     /locus_tag="JQH65_004015"
     CDS             44..1270
                     /gene="dsdA"
                     /locus_tag="JQH65_004015"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HAY6141231.1"
                     /translation="MAEGLPYVGLTEQDVQDAHARLSRFAPYLAKAFAETAATGGIIE
                     SELVAIPAMQKRLEKEYQQPISGQLLLKKDSHLPISSSIKARGGIYEVLAHAEKLALE
                     AGLLTLDDDYSKLLSPEFKQFFSQYSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADA
                     RAWKKAKLRSHGVTVVEYEQDYGVAVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAG
                     QRLKAQFAQQGRIVDADNPLFVYLPCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSP
                     CMLLGVHTGLHDQISVQDIGIDNLTAADGLAVGRASGFVGRAMVRLLDGFYTLSDQTM
                     YDMLGWLAQEEGIRLEPSALAGMAGPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGG
                     GMVPEEEMNQYLAKGR"
     gene            complement(1378..>2183)
                     /gene="emrY"
                     /locus_tag="JQH65_004016"
     CDS             complement(1378..>2183)
                     /gene="emrY"
                     /locus_tag="JQH65_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HAY6141232.1"
                     /translation="VISLISLVIWESTSENPILDLSLFKSRNFTIGIVSITCAYLFYS
                     GAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNKIDMRLLVTFSFLM
                     YAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFSGLPDNKFANASSM
                     SNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSSSQIMDKYYGSLSG
                     VLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTAKGVG"
BASE COUNT          599 a          508 c          536 g          540 t
ORIGIN      
        1 gtcacggcga actgcctttc aaggttgaag tggcacgacc tcattggctg aaggtttacc
       61 ttatgttggc ctgaccgaac aggatgttca ggacgcccat gcgcgcttat cccgttttgc
      121 accctatctg gcaaaagcat ttgctgaaac tgctgccact ggggggatta ttgaatcaga
      181 actggttgcc attccagcta tgcaaaaacg gctggaaaaa gaatatcagc aaccgatcag
      241 cgggcaactg ttactgaaaa aagatagcca tttgcccatt tccagctcca taaaagcacg
      301 cggcgggatt tatgaagtcc tggcacacgc agaaaaactg gctctggaag cggggttgct
      361 gacgcttgat gatgactaca gcaaactgct ttctccggag tttaaacagt tctttagcca
      421 atacagcatt gctgtgggct caaccggaaa tctggggtta tcaatcggca ttatgagcgc
      481 ccgcattggc tttaaggtga cagttcatat gtctgctgat gcccgggcat ggaaaaaagc
      541 gaaactgcgc agccatggcg ttacggtcgt ggaatatgag caagattatg gtgttgccgt
      601 cgaggaagga cgtaaagcag cgcagtctga cccgaactgt ttctttattg atgacgaaaa
      661 ttcccgcacg ttgttccttg ggtattccgt cgctggccag cgtcttaaag cgcaatttgc
      721 ccagcaaggc cgtatcgtcg atgctgataa ccctctgttt gtctatctgc cgtgtggtgt
      781 tggcggtggt cctggtggcg tcgcattcgg gcttaaactg gcgtttggcg atcatgttca
      841 ctgctttttt gccgaaccaa cgcactcccc ttgtatgttg ttaggcgtcc atacaggatt
      901 acacgatcag atttctgttc aggatattgg tatcgacaac cttaccgcag cggatggcct
      961 tgcagttggt cgcgcatcag gctttgtcgg gcgggcaatg gtgcgtctgc tggatggctt
     1021 ctataccctt agcgatcaaa ccatgtatga catgcttggc tggctggcgc aggaagaagg
     1081 tattcgtctt gaaccttcgg cactggcggg tatggccgga cctcagcgcg tgtgtgcatc
     1141 agtaagttac caacagatgc acggtttcag cgcagaacaa ctgcgtaata ccactcatct
     1201 ggtgtgggcg acgggaggtg gaatggtgcc ggaagaagag atgaatcaat atctggcaaa
     1261 aggccgttaa taacgtttca acgcagcatc gcaatccttt ccctgggtga gcgatgctgc
     1321 cgatggcgca gacttaagat ccccggtctt acccgctata accccctcct tttatcatca
     1381 cccaacgcct ttcgctgtaa acggcggttt cgcaaaccaa accaaaaccg taagtaagat
     1441 aaaagcaata gccgccatac ggaaaatctc atttgcagaa atagaaagtg actgctgggt
     1501 tatttcatta ttaatttcat taagaactcc tgaaagcgaa ccataatatt tatccataat
     1561 ttgtgacgat gaattaaaca cggggttaaa ttgatcgatg gttgctgtca actgactatg
     1621 gtgtaacgat tcgcgtcgtc cccacagcgt cattgtcaac gacgtaccaa ctgatcctga
     1681 caaggtacga aaaaaattac tcatactcga ggcattggca aatttattat ctggcaagcc
     1741 tgaaaacgaa atcgttgtta agggtaaaaa gaaacaggca acggcgaatc cctgaaaaaa
     1801 ctgcggcaaa atgatgcctg taaaatcaat cgttggcata aatgtcacag aacgccagta
     1861 atagcaaacc gcatacatca aaaaactaaa tgtcactaac aaccgcatgt ctattttgtt
     1921 gccataacgt cctatcaaag gtgaaattaa tagtggcatg atgccgatgg gcgcataagc
     1981 aagtccggcc catatcgcat tataccccat cgtttcctgg agtaactgcg gcataaggac
     2041 gatcgctcca gagtaaaata aatacgcgca tgtgatactc acaataccaa tggtgaagtt
     2101 acgggactta aacaaactga gatcaagaat cgggttctct gaggtcgact cccaaatgac
     2161 taaagagatc agagaaataa ctg
//
LOCUS       DADLFA010000299         2174 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000299 DADLFA010000000
VERSION     DADLFA010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.301"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            334..557
                     /locus_tag="JQH65_004017"
                     /pseudo
     CDS             334..557
                     /locus_tag="JQH65_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            666..934
                     /locus_tag="JQH65_004018"
                     /pseudo
     CDS             666..934
                     /locus_tag="JQH65_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1326..>2174
                     /locus_tag="JQH65_004019"
     CDS             1326..>2174
                     /locus_tag="JQH65_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY6141233.1"
                     /translation="MIKSTNIQAIGSGIMHQINNIYSLTPFPLPMELTPSCNEFYLKA
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDSIPPFPDKLEGLAMANNFIEQLPELPFSMNRAVL
                     MNNNLTTLPESVLRLAQNAFVNVAGNPLSGHTMRTLQQITTGPDYSGPRIFFSMGNSA
                     TISAPEH"
BASE COUNT          727 a          465 c          364 g          618 t
ORIGIN      
        1 gcttcttacc atatttgaaa taaagctcaa tagcgcaaat tttttgttct tcagtaaaca
       61 tgtttcatct tctgagagtc caggaaatcg tccgcatccc cattaccgtc ttatatcact
      121 ggcgctgaca tgtgctcatt gatgaagtga atctgaatga catgttttgc catatcaacg
      181 ccaaccggtg tatatttcat ttgtggagtc tccagtctga ggacgcttta tgcatcccgt
      241 attgggcagt atgatgccgg aaagctgcga ggctccacac ctgccagttt caccctttta
      301 accatcatac tactccgtgt ttaattgtgt gggatgcgtt cattacattt taacagtgtc
      361 accccgtcgg ccacgaaaga taaacaggtg accagaaaag ggatcttcat ccagcacatg
      421 ctatatcttg aacgtaacaa cctgaccctc cgcacccgca tcaagcggct ggcacgtaaa
      481 acaatctgct tctcgcgttc cgtggaaatc cacgaaaagt catcggctcc ttcattgaaa
      541 aacacatatt ctactgattg gaagcgccac ccgaaaaagt atcgtttttt tacagtaaac
      601 ttcatttagc cgactataat gtaaaaaaat aaatttttta ccgaaatata acataaggaa
      661 gtaatatgct tacacaaact atattccctt gtcttcccaa aaaacaagaa aatatcatcc
      721 ctgaagtatc aaatactata ttagcatctt ctactgttac tacagatgga tgtactgttt
      781 ttaataaaca aaaacagcca tatacgaatt gcaaatacct gctaccaacc gaacgaaaaa
      841 gttgaaattc acagctacag aaatacaatg gttgacaaag ataaatgaag ccggaattaa
      901 tgaaaaacaa agccaacggt attctgattt ctgaagttaa tttcagactc gtttaagctc
      961 cattttcaaa aaaaatatat cagggtttgt tactaataac ttctgaaaac agaaaaaaaa
     1021 tagagcagta attctatttc agagaggagt aactccatct atcctcgata cgtactgaac
     1081 gttatcgtga tatattcatc atgctttctt caccattatt cgagtacagg gagagacgca
     1141 atgaccttaa ataccttttt atggctgaat taccattctc aggaataaca gcagactcct
     1201 tcccatagac ggcttagggg gaaagcaata cccggagaaa gggtacaaag caaactgttt
     1261 taaaattaat aagctatatt ctatttcact agaattctta tcgctgataa gaaattttag
     1321 ttataatgat taaatcaacc aatatacagg caatcggttc tggtattatg catcaaataa
     1381 acaatatata ctcgttaact ccatttcctt tacctatgga actgactcca tcttgtaatg
     1441 aattttattt aaaagcctgg agtgaatggg aaaagaacgg taccccaggc gagcaacgca
     1501 atatcgcctt caataggctg aaaatatgtt tacaaaatca agaggcagaa ttaaatttat
     1561 ctgagttaga tttaaaaaca ttaccagatt taccgcctca gataacaaca ctggaaataa
     1621 gaaaaaacct attaacacat ctccctgatt taccaccaat gcttaaggta atacatgctc
     1681 aatttaatca actggaaagc ttacctgcct tacccgagac gttagaagag cttaatgcgg
     1741 gtgataacaa gataaaagaa ttaccatttc ttcctgaaaa tctaactcat ttacgggttc
     1801 ataataaccg attgcatatt ctgccactat tgccaccgga actaaaatta ctggtagttt
     1861 ctggaaacag attagacagc attcccccct ttccagataa gcttgaaggg ctggctatgg
     1921 ctaataattt tatagaacaa ctaccggaat taccttttag tatgaacagg gctgtgctaa
     1981 tgaataataa tctgacaaca cttccggaaa gtgtcctgag attagctcag aatgccttcg
     2041 taaatgttgc aggtaatcca ctgtctggcc ataccatgcg tacactacaa caaataacca
     2101 ccggaccaga ttattctggt cctcgaatat ttttctctat gggaaattct gccacaattt
     2161 ccgctccaga acac
//
LOCUS       DADLFA010000300         2146 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000300 DADLFA010000000
VERSION     DADLFA010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.302"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..664)
                     /gene="ipaH9.8"
                     /locus_tag="JQH65_004020"
     CDS             complement(<1..664)
                     /gene="ipaH9.8"
                     /locus_tag="JQH65_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HAY6141234.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HAL"
     gene            complement(926..1021)
                     /locus_tag="JQH65_004021"
                     /pseudo
     CDS             complement(926..1021)
                     /locus_tag="JQH65_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1057..1362)
                     /gene="ccdB"
                     /locus_tag="JQH65_004022"
     CDS             complement(1057..1362)
                     /gene="ccdB"
                     /locus_tag="JQH65_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HAY6141235.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1364..1582)
                     /gene="ccdA"
                     /locus_tag="JQH65_004023"
     CDS             complement(1364..1582)
                     /gene="ccdA"
                     /locus_tag="JQH65_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HAY6141236.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
BASE COUNT          572 a          452 c          499 g          623 t
ORIGIN      
        1 gcagagcatg ggatgataat gggttatcac taatatgtat tgaacattca ttcctcagat
       61 taagaatgct ttccgggata tgacttatct gatttctatc aaaaaaatac tctctgacaa
      121 cgggattatt tccctcagaa aatgctggta gctcagtgag gaaattacgg gtcgctctga
      181 gatttttaag agcctgtggg agcgatggta aggagactat ttcgttatag ctgatattca
      241 tagtcaataa cgaatcgggt aaagctggca ggttttccag ctcattgtgt tgtacctgaa
      301 gtgactccag cgcaggaggt agcacgggca attctgataa tttattgctg gcggaatata
      361 atttttttag cgtaacaggc agttcaggta ggttagttaa ttgattatat gatacattga
      421 gcagcgttat ctgagctggt aagttgtcag gtagcgagga cagatttaaa cggtccagtc
      481 gaagttcatc ggaattattg ataagacatt ctttaagtcg ggagacagcc tcatcacgct
      541 cttcaccggg gagcgcttgt ttttcccatt tatcccatgc tgaaaagtaa tcagcatatg
      601 taccggaaat agtgttataa aaagaatttt ggggcaatga aaagttatta tttatcggta
      661 acatgatggg catccagtta aagccggtgg acaaatggct ttgttaaaag aaataagcag
      721 gagtttttat gaagttcatt ccatgaaatg aacttcagca cttttttatt ataataagga
      781 taaacaaaat tggctgtaaa aaaacgatac tgtttcagtt aaccgtgaat ggcctgtttg
      841 caccgtattg ttcttgtttt tcggttagtt aaacatcgta cttcatattt gaacggtaag
      901 cggctcgtca gaaccgtatt gatatttact gagaggtcag atcaactttc cagggcaaca
      961 gatcgcgtac ccggtttgcc ggccagtctt ggatatgctc aatgacgtaa cgcagccatt
     1021 ttgtcaacgc cacgattttt gaccctagcc tgacatttat attccccaga acatcaggtt
     1081 aatggcgttt ttgatgtcat tttcgcggtg gctgagatca gccacttctt ccccgataac
     1141 gaagatcggc acactggcca tatcagtggt catcatgcgc cagctttcat ccccgacatg
     1201 caccaccggg taaagctcac gggagacttt atctgacagc aggcgggcac tggccagggg
     1261 gatcaccatc cgtcgtccgg gcgtgtcaat aatgtcactc tgtacatcca caaacagacg
     1321 ataacgactc tctcttttat aggcgtaaac cttaaactgc atttcaccag tccctgttct
     1381 catcggcaaa agagccgttc atttcaataa aacgggcgat ctcagccatc ccctgctgat
     1441 tttcagcctg ccagcgttcc gcacgcagac ggcgggcctc tttttgcatg gccgtattta
     1501 ccaagcctga gatattcacg tttgcggatt taagtaactg atagctgtca ctgtctatag
     1561 tgacagtaat acgctgcttc atgataagcc ctcaaaaaca tacttatgat atgtatatga
     1621 gtatatatta atggtacaaa ccatcaatat ccataaaaag tacaccatta attcactata
     1681 agtttttaat acaatttttt ctgctgcgat ttttttatac gataattagc atacttataa
     1741 caccgtaagt aaattacatg aaagcaggca cagcgtgatc cgatttttct gcgtataacg
     1801 attttccgga agtgtaatac ggttgcgccc cgggccgggc acccttttct gttgttgtgt
     1861 acgggcccac agagaatggt accggaggcg ggctcagaag tactgagtcg ggtgatcgcg
     1921 tcaggccgcg aagtactgac acgggtattg gtgactggtg atgaagtact gccccgggta
     1981 tgatttcagg ctgattcagg ttctgattct ctgtcagtcc gcgtggttgc tggcctggct
     2041 gatttcctgt tgtcaggcgt tcaacaaccg actgcaggtt atagcccggt tcacgaacca
     2101 cggcagcctg gcggctgcaa atcggggtgt ggcagaatat gagctt
//
LOCUS       DADLFA010000301         2051 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000301 DADLFA010000000
VERSION     DADLFA010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.303"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(273..1727)
                     /locus_tag="JQH65_004024"
     CDS             complement(273..1727)
                     /locus_tag="JQH65_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HAY6141237.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKTNPNKATKALQKNILRVTNSSS
                     SGISEKHLDHCANTVKNFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYISEIIQKVHPLSSDERHLLSIIINSNFNFRHQSNSNLSNNILNIKSFD
                     KIQSENIQTHKNTYSEDIKEISNHDFVFFGVEISNHQEKLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIF
                     NTKDMRLGIGLHLIDFIRKSKDQGFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLS
                     TDNFKKIKLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNKKADIALYLLSK
                     FNFTKQDVAEMEKMNNNRYCNLYDVEYLLSKDGANYKVLEYFINNGLVDVNKKFQKAN
                     SGDTMLDNAMKSKNPKMIDFLLKNGAILGKRFEI"
BASE COUNT          615 a          364 c          343 g          729 t
ORIGIN      
        1 tcccgtaaag acaggagtca agttcccgct ccgacataac tgcgtgcaat ggattttgaa
       61 ggaaccgtca gcccattccc cggaggcaaa catgggctgg caaaagtgta gcggtattag
      121 gcacagctat ttaggaaaga gggaccatct ccatctcatt aaatggacca cgccagacaa
      181 ttcactgtca tgctgatgct gcactgtggg gacagaatca ctcatgatga cttcgataat
      241 cgacgacatt attatttggc cgagctttta ggtcatatct caaatcgttt gcctaatatt
      301 gctccatttt ttaataaaaa atcaatcatt tttggatttt tgcttttcat tgcattgtca
      361 agcatagtgt ccccactatt tgctttttga aattttttgt ttacatcaac caatccatta
      421 ttgataaaat attctagcac tttatagttg gcaccatctt tactaagtaa gtattcaaca
      481 tcatacagat tacaatatct attgttgttc attttctcca tctctgcgac atcttgtttt
      541 gtaaaattaa atttagatag taaataaagt gctatatcag cttttttatt gcctaaagaa
      601 taagcaaggg cctgatgagc cattttttta tcagtcactt tattgttaat ctcttcataa
      661 ttagatgctt taatcgcatc ctccaaagat atgtctctga gttttatttt tttgaagtta
      721 tctgtactta gcattcttgg tatatgatac tcaagctgaa acacaaagtt tataattcta
      781 tccagactaa caggatcaat atttttgtta taacaaaact ctctgaatcc ttgatcttta
      841 ctctttctta taaaatcaat aagatgcaac ccaattccaa gcctcatatc tttagtgtta
      901 aatattggaa catcagtttt cccctgatta ccatgcacat acctgctaac ttcatccaca
      961 acctctttaa agttatctaa gaaaaatgat tggtgctcat ggtaaaaaac aggtggaata
     1021 gcattatcaa agtgatcggt taatgtcata tatccatatg gagagtcatg atcaatgata
     1081 tacgcattgg cgccaaaatc tacagtatga tgtgttttat ttagcgggag tttttcttga
     1141 tgattggaaa tttcaacccc aaaaaaaaca aaatcgtggt tagagatttc tttaatatcc
     1201 tcagagtatg tattcttatg tgtttgtata ttttcagact gaattttatc aaaggattta
     1261 atattaagaa tattatttga cagattagag ttactttgat gtctgaagtt aaaattagaa
     1321 ttaataataa tagaaagtaa gtgacgttcg tctgaagaaa gaggatgaac tttttgtatt
     1381 atctcagaga tatagtgcct ggacacctta tcgagatgct caagtgaccg aaatgatgac
     1441 cgtgctccag atggcgcttc aatagaaaga tttagtgatt tgaaactggt cccttgagaa
     1501 aacattttgc tataactttg cgctgctata gacttcctta aaaagttttt cacagtatta
     1561 gcacagtgat ctaaatgttt ttcacttatg ccagaagaag atgagtttgt tacgcgtaat
     1621 atattttttt gtaatgcttt tgttgcttta ttcggattcg tttttccatc aacaagatca
     1681 atattattct gaacattaat atgatttact gcttcaggta ttttcatccc ccccccaatt
     1741 atgtgcgttt ttatctgtgg ttatttagat aacattatct aactggacac tttcttgtat
     1801 gtattatagc taaatgaaca gattttaata tgttgtatgg ttttcactct gaaatcccct
     1861 gaatttaatc acgtttcctt tactgaaggc aattttaacc gtagtatcaa ttattaaccc
     1921 ttgggcttga tatccctctc ctcttctggg cacgggggat gtcagattac ttattacaga
     1981 aaccaaaaga attgtaagcg tcatctcagt gtcgtctatt tcaatgtctg atgattggcg
     2041 atgatagagt c
//
LOCUS       DADLFA010000302         2014 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000302 DADLFA010000000
VERSION     DADLFA010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.304"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..247
                     /locus_tag="JQH65_004025"
     CDS             <1..247
                     /locus_tag="JQH65_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141238.1"
                     /translation="HARLFGFTAEDIMDFWQHKAPQKYSAFELAFEFGHRVIAELILN
                     TLNKMAESFGFTDNPRYIAEKNYMEALLKKASPHTVR"
     gene            complement(216..1940)
                     /gene="aceK"
                     /locus_tag="JQH65_004026"
     CDS             complement(216..1940)
                     /gene="aceK"
                     /locus_tag="JQH65_004026"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HAY6141239.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     LAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAALVEWLREILPGKTT
                     AELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRVFKVI
                     KDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMELLLQE
                     AAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAAANIF
                     PGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSISPGD
                     VFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRRQRFS
                     VRYGEMLF"
BASE COUNT          508 a          530 c          510 g          466 t
ORIGIN      
        1 ccatgcacgc ctgtttggat ttaccgctga agatattatg gatttttggc aacacaaagc
       61 gccacaaaaa tactctgcct ttgagttggc ttttgaattt ggtcaccggg ttattgctga
      121 attaatcctt aatacattaa ataagatggc tgaaagcttt ggctttacgg ataaccctcg
      181 atacattgcg gagaaaaatt atatggaagc tttactcaaa aaagcatctc cccataccgt
      241 acgctaaatc tttgcctgcg ccgatacgca taaacatctt ccacatgccc ttcacgtatg
      301 cggttttgta gtgcgcgcca gtaatcagcg cggaacaggt cggcgtgcat ctcttcaaac
      361 agcggaccaa tacgcgggtc ggcgcatagc cagtggcgaa actcttccgg gaaaacatcg
      421 cccggcgaga tgctgtacca cggttcgctg gcaagttcgt cttccggata gcgcggcggc
      481 gggatgtcgc ggaaattcac ttccgtcatg tagcaaattt catcgtaatc ataaaaaacc
      541 acacgcccgt gacgggtgac accgaagttt ttaaacagca tgtcgccagg aaaaatgtta
      601 gcagcggcaa gctggcgaat agcgttaccg tattcttcaa tggcgtcgcg caactgctga
      661 ccttccactt gttccagcca gatattgagc ggcaccatcc gccgctcgat atagagatgg
      721 cgaattacaa tctgctcacc aagatcggtg attttttccg ctgcttcctg gagcagtaat
      781 tccattaatg ccggggaaat atgccgcttc tccagcacaa agttttcaaa ctcctgggtg
      841 tccgccattc ggcccacacg gtcgtgctct ttcaccagtt gatagcaggc acgaacgtga
      901 gcggcagaca tctctttctg cggcgcgaac ttgtctttga tgactttgaa tacccgatca
      961 aagcccggca gcgtaaacac caacatcacc attccacgaa tacccggcgc ttcaatgaac
     1021 tgctcattac agccctgtag ataaacgaga tattcgcggt agctttcggt tttggcgtgc
     1081 ttctggcagc cgatagccat atacaattca gcagtggttt tacctggcag aatttcccgt
     1141 agccactcga ccagcgctgc ataaaccata aaataagaac gcgcaaagcc aaaaacaatg
     1201 ctcgcttcgg cggtcgtcgt caggcaggta tcaataaata actcgccgtc gtccgtctgg
     1261 tggatcggca gtaagaaggg caacgtgccg gaaggcgtga tcagtttgcc caccagccag
     1321 gcggctttat tgcggtaaaa cagttcgttc gccacctgta aatgactttc cgcgaggttg
     1381 tctgtcccca gcgtttccgt cagatggcga atgatgtaat gaatgtcacg gcttttattc
     1441 tgccagcgca ggcgcagcgg caggtcgctg ataacgcgca tcagtagaga ttcccagccg
     1501 tgatcggggt gaaagtcttt cgccagcggg cggggaatgg tacgaaagcg gcgctctggc
     1561 tgagagctaa agataaaaag ccgctcggga gtaagcgagc ggtggtcaaa taaccgacag
     1621 tacacggagt taaaaaagct ctccgcaatc tcgaagcgcg ggtaatccgg caacagccgc
     1681 gtgtaatgct ctttaacgcg cagtaaaaat gccgcgtccg tactttggcc gttagtaatg
     1741 cagcgcagtt gctccacgac cagaccaacg tgatgatcgt aaagatggat acggtttttc
     1801 atcgccagct ggacagcatg ccagtcggcc tgttcgaaac gctgctgcgc cccggaagtc
     1861 acttcgagga atcgaccata ctgagcgtcg aagccttgca aaatggtttg agcaatcagt
     1921 aattccaggc cacgcggcat ttagcgccct catcaggagc agagaattgc cggatgcggc
     1981 gtaaacgcct tatccagcct acgttcggca acgg
//
LOCUS       DADLFA010000303         1945 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000303 DADLFA010000000
VERSION     DADLFA010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.305"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..954
                     /gene="tuf"
                     /locus_tag="JQH65_004027"
     CDS             <1..954
                     /gene="tuf"
                     /locus_tag="JQH65_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAY6141240.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGII
                     KVGEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAK
                     PGTIKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMP
                     GDNIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLG"
     gene            1137..1331
                     /gene="bfd"
                     /locus_tag="JQH65_004028"
     CDS             1137..1331
                     /gene="bfd"
                     /locus_tag="JQH65_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HAY6141241.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            1404..1880
                     /gene="bfr"
                     /locus_tag="JQH65_004029"
     CDS             1404..1880
                     /gene="bfr"
                     /locus_tag="JQH65_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HAY6141242.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGTPNLQDLGKLGIGEDVEEMLQSDLRLEQEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(1877..>1945)
                     /locus_tag="JQH65_004030"
     CDS             complement(1877..>1945)
                     /locus_tag="JQH65_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAY6141243.1"
                     /translation="PFGPFLAAAGFVIGWDSLLIGR"
BASE COUNT          515 a          440 c          504 g          486 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aactggctgg cttcctggat
      361 tcttacattc cggaaccaga gcgtgcgatt gacaagccgt tcctgctgcc gatcgaagac
      421 gtattctcca tctccggtcg tggtaccgtt gttaccggtc gtgtagaacg cggtatcatc
      481 aaagttggtg aagaagttga aatcgttggt atcaaagaga ctcagaagtc tacctgtact
      541 ggcgttgaaa tgttccgcaa actgctggac gaaggccgtg ctggtgagaa cgtaggtgtt
      601 ctgctgcgtg gtatcaaacg tgaagaaatc gaacgtggtc aggtactggc taagccgggc
      661 accatcaagc cgcacaccaa gttcgaatct gaagtgtaca ttctgtccaa agatgaaggc
      721 ggccgtcata ctccgttctt caaaggctac cgtccgcagt tctacttccg tactactgac
      781 gtgactggta ccatcgaact gccggaaggc gtagagatgg taatgccggg cgacaacatc
      841 aaaatggttg ttaccctgat ccacccgatc gcgatggacg acggtctgcg tttcgcaatc
      901 cgtgaaggcg gccgtaccgt tggcgcgggc gttgttgcta aagttctggg ttaattacac
      961 gttaattagt tttgaattga aaagggcgct tcggcgccct ttttgctgcc cgtaatttga
     1021 gctcgaaata tttagtcgta attttgctcg ctgaactatg gcgcaaataa aactgtgcta
     1081 ttgtaatcat aatcattctc atttacactt tgcgcagaat ttgaacggga gcaatcatgt
     1141 acgtttgttt gtgtaatggt gtaagtgaca aaaaaattcg ccaggcagtc cgtcagtttc
     1201 atccgcaatc tttccaacaa ttgcgtaagt ttattcctgt gggaaatcaa tgcggtaagt
     1261 gcattcgcgc cgcgcgcgaa gtgatgcagg acgagttgat gcagatgcca gaatttaaag
     1321 agattgcctg aagaccacgc ttattttttg acatcccgct agccccatct acgctctaat
     1381 agtggaagcg gagggactat aaaatgaaag gtgatgttaa aatcataaat tatctcaata
     1441 aactattggg aaatgagctt gtcgcaatca atcagtattt tctccatgcc cggatgttta
     1501 aaaactgggg cctgatgcgt ctcaatgacg tcgaatacca tgaatccatc gatgagatga
     1561 aacacgctga taaatatatc gagcgtattt tgtttttaga aggaaccccc aacttacagg
     1621 atttaggcaa actggggatt ggtgaagatg tcgaggagat gctgcaatcc gacctgcgat
     1681 tagaacagga aggcgcgaag gatctgcgtg aggctatcgc atatgccgac agcgttcatg
     1741 actatgttag ccgcgatatg atgatcgaga tccttgctga tgaagaaggt catatcgact
     1801 ggctcgaaac tgaactggat ctcatcggta aaattggcct acaaaattac ctgcaatcac
     1861 aaattaaagt taaagattaa cggcctatca gcaaactatc ccagcctata acgaaacccg
     1921 cagccgccag aaatggccca aaagg
//
LOCUS       DADLFA010000304         1942 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000304 DADLFA010000000
VERSION     DADLFA010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.306"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(25..>1942)
                     /gene="pdeF"
                     /locus_tag="JQH65_004031"
     CDS             complement(25..>1942)
                     /gene="pdeF"
                     /locus_tag="JQH65_004031"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HAY6141244.1"
                     /translation="IIIIILCWGGYRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLF
                     LILFQFAAFVGLLASRENLVGVMPFNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPF
                     YLRSYYSQLKQQVDAKVTKKEFALWLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLP
                     LMMWGAMRYGYKLISLLWAVVLMISIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVN
                     YMAVLATRQRAVVRRIQRLAYVDPVVHLPNVRALNRALRDAPWSALCYLRIPGMEMLV
                     KNYGIMLRIQYKQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDSHLK
                     QFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRRGAM
                     YLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELISPD
                     SFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPFEVS
                     QLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASYARL
                     KNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREAVLS
                     LGIDYMQGYLIGKP"
BASE COUNT          567 a          471 c          438 g          466 t
ORIGIN      
        1 ttcattcaac gtatcaatta acggctacgg cttaccaata agataaccct gcatataatc
       61 gatccccaaa gagagcaccg cctcgcggat ctcttcgttt tcaacgtact ctgccactac
      121 ccgcattttc ttcattcgcg ccaggtggca aatcgatgcc actatctgat aatccagact
      181 atttgacaca atattgcgga taaaactgcc gtcaatttta agcagatcgg cattcacatt
      241 tttaagccgt gcatagctgg cgtagccggt gccgaaatca tcaatcgcaa tctggcagcc
      301 taattcctga agatgctgca aggtgatttg cgcctgctta acattggtca gagcattact
      361 ttcggtgact tcaaaaataa gttgccacgc ttcaatctga tatttagcca gcaactgact
      421 gacttcaaaa ggaaaacgag cctgacatac cgaggttgga gacagattaa tagcaaaacg
      481 gtgagcgggc atcttcgctc tgttttcagc cataaattgc agcgtatgct caatgaccca
      541 catgtcgata ctcgacgata aaccaaattc gtgcgcgacc ggcaagaagc tatcggggct
      601 gatcagttca tcattctcac ctttcatgcg cagaagaatt tcatggtaaa catcaccacg
      661 cataccggta atcggctggg ccatcaggaa aaaatggttg tgttccagcg cctgctgtag
      721 tcgattcatc atcgcgactt tatctttcaa ttcgcgttgc aaatacattg ccccgcgacg
      781 ctgcatattt tccggggcgt tggtcacgat ggaaagttcg gcgaccgtat ttagctctcc
      841 cagcagcagg tagatatgat tcactggcga gcgcacatag cagtaactga cgccaatctg
      901 cggttgcatc ggcatgccat cccaaaagaa acgaaattgc ttgagatggc tatccagtgc
      961 ggtaatgcgc tcctggtgcg attctgtatt cagtcgcagc gcgagatcgt tacccgaaag
     1021 ctgataaaca tcttcacccg gttccagcaa gggtgacagc cagtgagaaa gtttttgctt
     1081 gtattgaatc cgcagcatga tgccatagtt cttaaccagc atttccatgc cagggatgcg
     1141 taaataacaa agcgcagacc agggggcatc acgcaacgcg cgattcaggg cgcgaacatt
     1201 tggcagatga accaccgggt ccacatacgc aagccgctga atgcgtctga ctaccgctct
     1261 ctgacgggtt gccagtacag ccatataatt gacaataaaa gagaatacca gataactgga
     1321 ggaggttatg gtcagctgcg tggtatagcc aggataaatg ggaatgtaat tttgatagct
     1381 gtggatgctg atcatcaaca cgaccgccca gagcagagaa atcagcttat aaccatagcg
     1441 catcgctccc cacatcatca ggggcagcaa taatgacaag gtataattgg tgctaaaaat
     1501 tgtgcttttt tcatttaacg gcatgcataa cagcaacaat aaagcaccta atgccagtag
     1561 ccagagcgcg aactcttttt tggtgacttt ggcatcaacc tgctgtttta attgcgaata
     1621 atagctacgt aaataaaatg gatttcgcac tacccgaatg atgaagtagc acagcgggac
     1681 accgatcaga ttacccacca gtaaggcctg ataattgatt aaagtcccga ggttaaaagg
     1741 catgacaccg accagatttt ctctgctcgc caataatcct acaaatgcag caaactggaa
     1801 aagtatcaga aacagcgttg caggaaacac aatctgccag aatatacgct gggaaattaa
     1861 acgggtatca ccatgtgaaa cgttgttacg ccggggagta aagaccctgt aaccgcccca
     1921 acagagaatg ataatgataa ta
//
LOCUS       DADLFA010000305         1903 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000305 DADLFA010000000
VERSION     DADLFA010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.307"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(1..483)
                     /locus_tag="JQH65_004032"
     CDS             complement(1..483)
                     /locus_tag="JQH65_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY6141245.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRVVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCIQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(474..776)
                     /locus_tag="JQH65_004033"
     CDS             complement(474..776)
                     /locus_tag="JQH65_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAY6141246.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
     gene            1048..1320
                     /locus_tag="JQH65_004034"
     CDS             1048..1320
                     /locus_tag="JQH65_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141247.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
     gene            1301..>1903
                     /locus_tag="JQH65_004035"
     CDS             1301..>1903
                     /locus_tag="JQH65_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004150716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISSbo1 family transposase"
                     /protein_id="HAY6141248.1"
                     /translation="MTRSGGDFQPRPLKRLFTANQCWTSFLDAGGLRDIGVEAVTKML
                     ACGTRILGVKEYICDKPECPHVRYVTNSCGSRACPSCGKKATDLWIATQLNRLPDCDW
                     VHLVFTLPDTLWPVFESNRWLLNDVCRLAVENLLYAARKRGQEPGIFCAIHTYGRRLN
                     WHPHVHVSVTCGGLNKHGQWKKLSFLKDAMRSRWMWNMRQL"
BASE COUNT          514 a          490 c          458 g          441 t
ORIGIN      
        1 tcagacttta taaaacaagg tattaggtgc cacaactgac tgaacaaaac caaaacgctc
       61 ataaaatgct ctggcgtcat catcaattgc atgtaccata acagccttaa tgccaacctg
      121 atcagccaga ttgacaaccc ggtgaatggc atcacttagt agccatttac cgaaaccatg
      181 cccctggata caaacatcaa cagccagacg ccctaacaga acaacaggga caggattggg
      241 catgttatgc cgcaaactgc gaccaagctc ggcatgagtg acggaaccag tagctaatga
      301 gtagtatccc acaacacgta aagtatcctc caagcaaata acgaacgtgc gagaagcatt
      361 gagcatctgg tttttcatag cccgaccacg taaccagtta cttagcacct catttccaca
      421 gtcaaatggc tgaagtatat gctcatccgt caacaatgct ggcgcggtga catttatttc
      481 cattcaggct ttaccgccat cagacgttca cgcccttctg cattctgcac aggagcttcc
      541 agttgcgtaa taaacgaatc ataacgttcg ttatccagca caaataaacg ttgatccagg
      601 ataatgtttt tagcttcctg gtacgccagt tccatgatga aatcggtacg gttcttattg
      661 accaagctcg cagcataatc aataacggct cgttcctcat cagttgcccg gatgttaatc
      721 tgatgttctc gcggtgtttt acggacgctt gcagctgtag acataaaaac ctccattggt
      781 aacaatttgc tatgcaatta atgttatcgc aaatggtaac agaacgctat acagaatcac
      841 ttacgggaaa agcgatcccc tgataaaaac aatggcagag acctgaacat tgtgacgatg
      901 atctgtgtcg gcgcacaaat catgtctgat tacggccgtt taatgtgatt ttgataatga
      961 aatgcctgaa ccacggcagc ctggcggctg caaatcgggg tgtggcagaa tatgagcttt
     1021 cagagtaacc atccagaatg agttcccatg tcacacaacc tggagcacca gaaagtccac
     1081 accagaatgg tgaaagaggt tctgaaagcc gtcgccagag ccaataatca cccttataag
     1141 tccgtatttg cagactttat caccggtcat ccctcctgca cggtctgttt ctgggaaacc
     1201 ttccacaaaa tgtacccgga ttcaccgtac gaatacgtca cgttctgcca tacctgccgt
     1261 cgttttgatt tatacgaaac agaagcggag atgaaggcgg atgacccgaa gtggtggtga
     1321 ttttcagccc cgaccgctga aacgcctgtt cacagccaac cagtgctgga cgtccttcct
     1381 ggatgcgggc ggtctgcgcg atatcggggt tgaggctgtc acaaaaatgc tggcctgcgg
     1441 cacgcggata ctgggagtga aggaatacat ctgcgataaa cctgagtgcc cccacgtcag
     1501 atacgtcact aactcatgcg gcagccgtgc ctgcccgtcc tgcggaaaaa aggccacaga
     1561 cctgtggata gcgacacagc tgaatcgtct tcctgactgc gactgggtac acctggtctt
     1621 caccctgccg gacacgctgt ggccggtgtt cgaaagcaac cggtggctgc tgaatgacgt
     1681 gtgccgtctg gcggtggaga atctgctgta tgccgcccgg aaacgggggc aggaacccgg
     1741 tatcttctgc gccatccaca cgtatggccg tcgtctcaac tggcatccgc atgtacatgt
     1801 gtctgtaacc tgtggaggtc tgaataagca tggtcagtgg aaaaagctga gcttcctgaa
     1861 agacgcgatg cgttcacggt ggatgtggaa tatgcggcag ctg
//
LOCUS       DADLFA010000306         1897 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000306 DADLFA010000000
VERSION     DADLFA010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.308"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(68..1249)
                     /locus_tag="JQH65_004036"
     CDS             complement(68..1249)
                     /locus_tag="JQH65_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HAY6141249.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PCTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            1504..>1897
                     /locus_tag="JQH65_004037"
     CDS             1504..>1897
                     /locus_tag="JQH65_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141250.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVS"
BASE COUNT          495 a          514 c          420 g          468 t
ORIGIN      
        1 ttgccagcca tcattattca acagattaat gacggcagat atttatcggt aaggaggaaa
       61 tgcagagcta ctcgtcacgt tcaatggtca atgccacgcc ctggcccccg ccgatacaaa
      121 gcgttgccag ccctttgcgg gcattacgtt tcaccatttc atgaaccaga gaaaccagga
      181 ttcggcaacc ggaagcgcct atcgggtgac cgagtgcgat cgcgccacca ttgacattga
      241 cccgacgctc atcccactca agcatcttgc caaccgaaag cgcctgtgca gcaaacgctt
      301 cattagcctc gataagatcg acatcagcca actgccagcc tacacgctcc aggcaacggc
      361 gggtcgcata caccggcgca attcccatca atgccggatc tacaccaacg ctggcaaatg
      421 cgcggatgcg ggccagcacg ggtaaattca acgctcgtgc tttggcttcg ctcatcatca
      481 ttaccgcagc tgcgccatcg tttatggatg atgcattccc cgcggtcaca gaaccgagac
      541 tatcaaatga aggatttaaa cgggctaaac cttctgcact ggcgtcagtg catggctgtt
      601 catcggtatc aacaaccaac gtctgaccgt tactttggat cattaccggg acgatctcat
      661 ctttaaatcg tccggcgtca atcgccgctc gcgctttttg ttgcgagcta agtgcgtaag
      721 catcctgcaa ctgacggctg atgccatatt cgcgagccag attttcggcg gtgacaccaa
      781 tatgataatc attgaaggca tcccacaacc catcatgcac cagactgtca accaactggc
      841 tattgccaag ctgtgcaccg gtgcggctat cagtcagaac atgtggtgcg cggctcatgt
      901 tttcctggcc accggcgatg acaatatcag cctcgccaca ctgtatcgcc tgagtagcca
      961 gatgcagtgc tttaagccca gaaccgcaaa cgtcattaat agtgattgca gaaacgctat
     1021 taggaagacc acctttaata gccgattgcc ttgccggatt ctgccctgcc cctgcagtca
     1081 acacctgacc aagaattact tcatccaccg catatgcagg aacgccggta cgttctatta
     1141 acgctttcac gaccaaacta ccaagttcca cggcggaatg acccgctaac gcaccacgaa
     1201 agcagccgat aggtgtccgt aacgccccga caatcacaac gtctttcatc acaacctcgc
     1261 accaattaac agtgcaatag taaatgattg ttaattacag ttatcttaat tgtttaaaaa
     1321 aagtgatttt tatcacaaaa gaaatatgcc tgagcagcag tcagagacat aactggcacg
     1381 taaggtttgc aaccactaac ccaccaatag aggggtagat agggcgttaa tctcccatac
     1441 ttaacctggt ttatggtaaa ttgccctcca ttttgtttaa tttgtagatg atacgttcag
     1501 ataatgtcta atatttggtc taaagaagaa actctgtgga gtttcgcgct ctacggcaca
     1561 gccgttggtg caggcacgct cttccttcct attcagttag gttcggcagg ggctgtggtc
     1621 ctgtttatta ctgctctggt cgcctggcct ttaacatatt ggccacataa agctttatgc
     1681 cagttcatcc tctcatcgaa aacatcagca ggtgaaggga taacgggcgc ggtaacacac
     1741 tactatggca agaagattgg taatctgatt accacgctgt acttcatcgc cttttttgtc
     1801 gtcgtgttga tatatgcagt ggcaattacc aactcactta cagaacagct ggcaaagcat
     1861 atggttattg atcttcgcat ccgtatgttg gtgagtc
//
LOCUS       DADLFA010000307         1886 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000307 DADLFA010000000
VERSION     DADLFA010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.309"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..>1886
                     /locus_tag="JQH65_004038"
     CDS             <1..>1886
                     /locus_tag="JQH65_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HAY6141251.1"
                     /translation="FTAAHGNNTASKITNILDGTVTATSSDAINGSQLYDLSSNIATY
                     FGGNASVNTDGVFTGPTYKIGETNYYNVGDALAAINSSFSTSLGDALLWDATAGKFSA
                     KHGTNGDASVITDVADGEISDSSSDAVNGSQLHGVSSYVVDALGGGAEVNADGTITAP
                     TYTIANADYDNVGDALNAIDTTLDDALLWDADAGENGAFSAAHGKDKTASVITNVANG
                     AISAASRDAINGSQLYTTNKYIADALDGDAEVNADGTITAPTYTIANAEYNNVGDALD
                     ALDDNALLWDETANGGAGAYNASHDGKASIITNVANGSISEDSTDAVNGSQLNATNMM
                     IEQNTQIINQLAGNTDATYIQENGAGINYVRTNDDGLAFNDASAQGVGATAIGYNSVA
                     KGDSSVAIGQGSYSDVDTGIALGSSSVSSRVIAKGSRDTSITENGVVIGYDTTDGELL
                     GALSIGDDGKYRQIINVADGSEAHDAVTVRQLQNAIGAVATTPTKYFHANSTEEDSLA
                     VGTDSLAMGAKTIVNGDKGIGIGYGAYVDANALNGIAIGSNAQVIHVNSIAIGNGSTT
                     TRGAQTNYTAYNMDAPQNSVGEFSVGSADGQRQITNVAAGSADTDAVNVGQLKVTDER
                     VAQNT"
BASE COUNT          498 a          477 c          489 g          422 t
ORIGIN      
        1 ttcactgcag ctcatggcaa caataccgcc agcaaaatca ccaatatcct ggacggcaca
       61 gtcactgcaa ccagttccga tgccattaac ggtagccagc tttatgactt aagcagcaat
      121 atcgccacct acttcggcgg caatgcttct gtgaatactg acggtgtgtt taccggtcca
      181 acctacaaaa tcggtgaaac aaattattat aacgtcggcg atgcactggc tgcgattaac
      241 tcctcattta gcacgtctct cggcgatgct ctgctttggg atgccaccgc aggtaaattc
      301 agtgccaaac acggtactaa tggtgacgca agcgtgatca ctgatgtcgc agatggtgaa
      361 atttcagact ccagttctga cgcagtaaac ggctcacaac tccacggcgt gagcagttat
      421 gttgttgatg cgctgggggg tggtgccgaa gtcaatgcag acggcaccat cactgcgccg
      481 acgtacacca ttgctaatgc tgattacgat aatgtcggtg atgccctgaa tgctatcgat
      541 accactcttg acgacgctct gctctgggat gcggacgccg gtgaaaatgg tgcatttagc
      601 gccgctcacg gaaaagataa aactgccagt gtaatcacta acgtcgctaa cggtgcaatc
      661 tctgctgcca gcagagacgc gattaacggc tcacaactct ataccaccaa taagtacatc
      721 gctgatgcgc tggatggtga cgcagaagtc aacgctgacg gcaccatcac cgcaccgact
      781 tacaccattg cgaacgccga gtacaacaac gtcggtgacg ctctggatgc gcttgatgat
      841 aacgccctgc tgtgggatga gactgccaat ggcggtgctg gagcctacaa tgccagccat
      901 gacggtaaag ccagcatcat cactaatgtc gctaatggca gtattagtga ggacagtacc
      961 gatgcagtga acggttctca gttgaatgcg acgaatatga tgattgagca gaacacccaa
     1021 attatcaatc agctcgctgg taacaccgac gcaacctata tccaagaaaa cggtgcgggt
     1081 attaactatg tgcgtactaa cgacgacggc ttagcgttca acgacgccag cgcacagggt
     1141 gttggcgcta cagctatagg ttataactct gtcgccaaag gcgatagcag cgtagctatt
     1201 ggtcagggca gctacagcga cgttgatacg ggtatcgccc taggtagcag ctctgtttcc
     1261 agccgagtga ttgccaaagg ctcccgtgac accagcataa cggaaaatgg cgttgttatt
     1321 ggttacgaca ccacggatgg cgaactgctc ggtgcattgt ctatcggtga tgacggtaaa
     1381 tatcgtcaaa tcatcaacgt agccgatggt tccgaagccc atgacgccgt tacggttcgt
     1441 caattgcaga atgcgattgg tgcggtcgca accacgccga ctaaatactt ccacgctaat
     1501 tcaacggaag aagattcact ggcagtggga actgactcgc tggcaatggg tgcgaaaacc
     1561 atcgtgaatg gcgataaagg tattggtatc ggttatggtg cctacgtgga cgcgaatgca
     1621 cttaacggca ttgccattgg tagcaatgcg caagtcattc atgtcaacag tattgcgata
     1681 ggtaatggtt ctacgaccac tcgtggcgct caaaccaatt ataccgccta caacatggac
     1741 gcaccgcaga actctgtcgg tgaattctca gtcggtagtg cggatggtca acgtcagatc
     1801 acaaacgtcg cagctggttc agcggatacc gatgcggtta acgtgggtca gttgaaagtc
     1861 actgatgagc gcgtagcgca aaatac
//
LOCUS       DADLFA010000308         1883 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000308 DADLFA010000000
VERSION     DADLFA010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.310"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            368..1156
                     /gene="virF"
                     /locus_tag="JQH65_004039"
     CDS             368..1156
                     /gene="virF"
                     /locus_tag="JQH65_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HAY6141252.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
     gene            complement(1098..1508)
                     /locus_tag="JQH65_004040"
     CDS             complement(1098..1508)
                     /locus_tag="JQH65_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001409096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY6141253.1"
                     /translation="MPFHRPTKWVQYISAAPALSQQAVDQEWSYMDFLEHLLHEEKLA
                     RHQRKQAMYTRMAAFPAVKTFEEYDFTFATRAPQKQLQSLRSLSFIERNENIVLLGPS
                     GVGKTHLAIAMGYEAFKIFYDISKISLELYHNIH"
     gene            1519..1883
                     /locus_tag="JQH65_004041"
                     /pseudo
     CDS             1519..1883
                     /locus_tag="JQH65_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          591 a          337 c          374 g          581 t
ORIGIN      
        1 aatatcaaca ccaaccggat ttgtggagcc tccagtctga aggggcttta tgcgttccgt
       61 ataggatatt atgatgctgg agttttgcga agctccactc ctttccgaac atttacacag
      121 atattgccta agaaaagtga aaccataata tggttatagt ccctttcagt gcaaatactt
      181 agcttgttgc acagagaaat agaagctgca taagctcttt cttcaaaaaa tgtaaataaa
      241 gttaaatata ggaaaaatta cttaatctat cttaataacg gaaagatttt gtatacaatc
      301 actgttacag aaatttctta gttactctgt aaacactaaa tatagtttgg tatattctgt
      361 tgaatttatg atggatatgg gacataaaaa caaaatagat ataaaggttc gcttgcataa
      421 ctatattatt ttatatgcaa aaaggtgttc aatgacggtt agctcaggca atgaaacttt
      481 gactatcgat gaagggcaaa ttgcttttat agagcgaaat atacaaataa acgtctccat
      541 aaaaaaatct gatagcatta atccatttga gattataagc cttgacagaa atttattatt
      601 aagcattatt agaataatgg aaccaattta ttcatttcaa cactcctatt ctgaggagaa
      661 aagggggtta aacaaaaaaa tattcctcct ctctgaggag gaggtttcta tcgatttgtt
      721 caaatctata aaagagatgc cttttggcaa aagaaagatc tatagtttag cttgcctttt
      781 atcagctgtt tctgatgagg aagctttata tacttcgata tcgatagctt cttctcttag
      841 tttttctgat cagataagga agattgttga aaaaaacatc gagaagagat ggcgtctttc
      901 tgatatttca aataacttga atttatcaga aatagctgtt agaaaacgat tggagagtga
      961 aaaattaaca tttcaacaaa tccttcttga tattcgcatg catcatgcag caaagctttt
     1021 attgaatagt caaagctata ttaatgatgt atcaagactt atcggaatat caagcccatc
     1081 ttattttata aggaaattta atgaatatta tggtataact ccaaagaaat tttacttata
     1141 tcataaaaaa ttttaaatgc ttcatagccc atcgctattg ccagatgggt tttccccaca
     1201 cctgatggcc ctagtaatac gatattttca ttacgttcta tgaagctgag tgagcgtaac
     1261 gactggagtt gcttctgcgg tgctctggtg gcgaatgtga agtcatactc ttcgaacgtt
     1321 ttcaccgccg ggaaggctgc cattcgggta tacatcgcct gtttacgttg atgacgtgcc
     1381 agtttttctt catgaagcag atgctccagg aagtccatat aactccattc ctggtctact
     1441 gcctgttgtg acagcgcagg cgctgcgctt atgtactgca cccattttgt tggacgatga
     1501 aatggaatag cccctaatat gtcaaagcca aaataccctt ttgaaaagcg ccttgaagtc
     1561 gtgaatcact acttcactac tgatgatggt tacaggatca tctcggcacg ttttggtgtc
     1621 cccgaaccca ggtcaggaca tgggttgccc tctatgaaaa acatggagaa aaaggcttaa
     1681 ttcccaaacc taaaggtgtt agtgctgatc cagagttgcg tattaaggtc gtgaaagctg
     1741 tgattgagca gcacatgtcc cttaatcagg ctgctgctca ctttatgctt gctggtagtg
     1801 gttctgtagc caggtggctg aaggtttatg aagagcgcgg agaagctggt ttacgcgcgc
     1861 tcaagattgg caccaaaaga aac
//
LOCUS       DADLFA010000309         1816 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000309 DADLFA010000000
VERSION     DADLFA010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.311"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(316..1464)
                     /locus_tag="JQH65_004042"
     CDS             complement(316..1464)
                     /locus_tag="JQH65_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY6141254.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWSIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMLASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT          574 a          464 c          396 g          382 t
ORIGIN      
        1 ggttggcgta gttattaaca acgttgccaa aaccccagac agtgataaaa gacatcagcg
       61 caatgttgta ccatcgcagc tgtttttcct gaacattccc agccatagac aaatccttcg
      121 ccaaaattat aggggcatct gtaaaacgag ggattatgcg cggcaaataa gaagtgcgga
      181 aatagcgaaa tgcaaaatta taacctgctg cacattttat ttttaaacga ataaaatttg
      241 gaataaagca agtcagatag aagaaacaaa aaagcagagt gtgaaacagt aagttcacac
      301 tctgcttgaa ttcatttaca cgtgagcaac gggtttcggc gtatgaccgg cgttgcgcag
      361 cagcatcagc aaatagataa acgatacgct ggcgagcatg ataaacaata aattatcgga
      421 gaaattctgc attagcatag cggtaaatga cgggccaagc agacttccca cagtatagct
      481 caacagtaag gcctggttca tcgccaccag ttgatgatgt tcaactttct cgcaagccca
      541 tgccatcgcc accggatata gcgtaaagcc agcggcaccg aggatgaata acgctggggc
      601 catcgccgcc tggctaagca tcgcgatact gccgagaatg acgacaaaga cctgaacacg
      661 caacaccagc agtcgaccaa acttatccgc cagacgtcca atcgaccatt gtccaaggat
      721 acccgcactg accagtaccg ccatccagaa accaatgctg gcattgctca cccctttgtg
      781 attgaggtac agcggcatca ggccatatag agatcccaga acgattcctg agataatgca
      841 gccattcacg ccaagccgcg cctgacggag ttttagcatt gacgtaattg acgtcgaatc
      901 atggttttca gcctgctgat tcagcacacg cgtaaacaac agcggtaaga tccctgccaa
      961 cgtcaaacct gtaacccacg gcaatacgga catcagctcg gttgaaactt tgctgaccag
     1021 taactggcct aaaaacgttc ccacgtaata aaccatcata tacgcagcaa gcaaacgccc
     1081 acggttacgt gacgtcccac tgcacatcag cgcgctctca acaaccaccc aaatcatggc
     1141 acagccgacg cccgcgacaa aacgccaagc caaccagctc cagaatccaa tcatcaggcc
     1201 aaggccggca cagccagcgg caaaaatgaa ggaggccaga taatagctgc ggttaaagcc
     1261 aatgcgctta atgacatacc ctgtcagcaa tgtaccgaca aggttgccgg taaaatagga
     1321 tgagctgaca acgcctacct gccatgtgga catgtgttcc tgggcgagcc aaagcggcac
     1381 gagtgtattt aacaccgcaa tcgccagagt caacaaaagc aggccagaca gcaaaagcat
     1441 gacaggccgg gtatacgtgg acatgaatta aaaccgtgag gaagttcaaa tttcatgcgc
     1501 atcatgccac cgccaaaaca ttgtcaatga gtgaaaatac gcgatatccc agcggcggta
     1561 ttatcgattt atattactca ttcataacgc taatgctgaa gagatgatta gtctcatctg
     1621 attgttttta ttgaactaac acaaatttca tacgcttcgt tcagtcgaaa aatttcatgg
     1681 tcacagcaaa ctcatatttc ccgtctatgc tttcagagca atgcatcgcg tctgatcgat
     1741 ttcagacgta ccactgactc tcgaaaggag aagaggatga ccaaaccgta tgttcgtctt
     1801 gataaaaatg atactg
//
LOCUS       DADLFA010000310         1762 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000310 DADLFA010000000
VERSION     DADLFA010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.312"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..277
                     /locus_tag="JQH65_004043"
     CDS             <1..277
                     /locus_tag="JQH65_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309007.1"
                     /note="oxidoreductase, Fe-S subunit; terminal electron
                     transfer protein for the reduction of DMSO; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit B"
                     /protein_id="HAY6141255.1"
                     /translation="QYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDEL
                     RKKHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            279..1142
                     /locus_tag="JQH65_004044"
     CDS             279..1142
                     /locus_tag="JQH65_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HAY6141256.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLIVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(1177..1758)
                     /locus_tag="JQH65_004045"
     CDS             complement(1177..1758)
                     /locus_tag="JQH65_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005135793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAY6141257.1"
                     /translation="MLVDHQAGLLSLVRDIEPDKFKNNVLALGDQAKYFNLPTILTTS
                     FETGPNGPLVPELKAQFPDAPYIAHPGNINAWDNEDFVKAVKATGKKQLIIAGVVTEV
                     CVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGAQLMTWFGVACELHRD
                     WHNDIEGLATLFSNHIPDYRNLMTCYDTLTKQK"
BASE COUNT          379 a          423 c          490 g          470 t
ORIGIN      
        1 gcaatataac gaaacgaaag gccatatgac caaatgcgat ggttgttatg accgtgttgc
       61 ggagggtaaa aagccgatct gtgttgaatc ttgtccgctg cgggcgctgg atttcggccc
      121 tatcgacgag ctgcgtaaaa aacatggcga tctggcggcc gttgcgccgt tgccgcgagc
      181 tcactttacc aaaccgaata ttgtgatcaa acccaatgcc aatagccgcc cgaccgggga
      241 taccactggc tatctggcaa acccgaagga ggtgtgagat gggaagtgga tggcatgaat
      301 ggccgctgat gatcttcacg gtcttcgggc aatgtgtagc aggtggtttt atcgttctgg
      361 ctttggcgct gctcaaaggc gacctgcgag cagaagccca gcagcgtgtt atcgcctgca
      421 tgtttggttt atgggtgctg atgggcattg gctttatcgc ctctatgctc catcttggtt
      481 caccaatgcg cgcttttaac tcgctcaacc gggtaggggc ttcagcactc agtaacgaaa
      541 tcgccagcgg ttcgatcttt tttgccgtag gcggcatcgg ctggttgctg gcaatgctga
      601 aaaagctgtc accggcattg cgtacgctgt ggctgatagt gacgatggtt cttggcgtca
      661 tctttgtctg gatgatggtg cgtgtgtata acagcattga taccgttccg acctggtaca
      721 gcatctggac gccgatgggc ttcttcctga cgatgtttat gggcggcccg ttactgggtt
      781 acctgttgtt gagtctggct ggcgtcgatg gctgggcgat gcgtctgctg ccagcaattt
      841 ctgtactggc actggtagtg agtggcgtgg tgtcagtgat gcagggcgca gagctggcga
      901 ctattcatag ttctgtgcag caggccgcag cgctggtgcc ggactatggt gcgctgatgt
      961 cctggcggat cgtgcttttg gccgttgccc tgtgcttgtg gattgcacca cagctaaaag
     1021 gttatcagcc tgcggttccg ctactttctg tatcattcat tctgcttctg gcaggggaat
     1081 taatcggtcg cggcgtattc tatggtttgc atatgaccgt ggggatggcc gtcgcaagct
     1141 aatcataaca accggggttt cggccccggt tctcttttat ttctgcttcg ttaacgtgtc
     1201 ataacaggtc atcaaattac gataatcagg aatgtggtta gagaacaacg tcgccagtcc
     1261 ttcaatatca ttatgccagt cgcgatgcag ctcgcacgcc acgccgaacc atgtcatcaa
     1321 ttgcgcacca gcttgcgaca tgcgatccca tgccgaatgc cgggtaattt cattaaaggt
     1381 ccctgacgcg tcagtcacga caaagacgtc aaacccttct tcaatggctg aaagcgccgg
     1441 gaatgccacg caaacttcgg ttaccacacc ggcaataatt aactgttttt tacctgtcgc
     1501 tttgacagct tttacaaaat cttcgttatc ccaggcgtta atatttcccg ggtgagcaat
     1561 gtaaggtgca tcggggaatt gtgctttcag ttctggaact aatgggccgt taggaccggt
     1621 ttcaaaactg gtggtaagaa tagttggtaa gttgaaatac ttagcctggt cacccagcgc
     1681 cagcacgtta tttttaaact tatcgggttc gatatcccgt acaagggaaa gtaaaccagc
     1741 ctggtgatca acaagcaaaa cg
//
LOCUS       DADLFA010000311         1736 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000311 DADLFA010000000
VERSION     DADLFA010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.313"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            67..807
                     /locus_tag="JQH65_004046"
     CDS             67..807
                     /locus_tag="JQH65_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HAY6141258.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGTIEGNASL"
     gene            842..1736
                     /locus_tag="JQH65_004047"
                     /pseudo
     CDS             842..1736
                     /locus_tag="JQH65_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT          502 a          367 c          369 g          498 t
ORIGIN      
        1 aatagtgtta ataatttcta tttttatatt attccctgtt ttaattaact ctgtcaggga
       61 tggtttatgt tttttaacac caaacataca acagctttat gcttcgtaac ctgtatggct
      121 tttagttcat cgtctattgc ggacattgtc atttcgggta ctcgcgtaat atataaaagc
      181 gatcaaaaaa gtgtcaacgt acgtctggaa aataaaggga ataacccgtt gcttgtccag
      241 agttggttag atactggcga tgacaacgct gagcctggca gtattacagt cccttttact
      301 gccacgccgc cagtatcgcg tattgatgcc aaacgtgggc aaacaatcaa attaatgtac
      361 acagccagca cctcactgcc taaagacaga gagagcgtgt tctggtttaa cgtactggaa
      421 gttccaccaa aaccagatgc agaaaaggtc gcgaatcaaa gtctgctgca actggcattt
      481 cgcacacgta taaaactttt ctatcgcccg gatggattga agggaaatcc ctctgaagcc
      541 ccgttagccc tgaagtggtt ctggtcaggt tcagaaggta aggcgtcatt gcgagtgacc
      601 aatccaaccc cttactacgt ctcttttagc agtggtgatt tagaagctag cggtaaacgc
      661 tatccgattg atgtgaaaat gattgcacca tttagtgatg aggtcatgaa agtcactggc
      721 cttaatggca aagcaagttc tgcaaaagta catttttacg ccattaatga ttttggtggc
      781 accattgaag gtaatgccag tctgtaatca ggcaaggata taattccgca ggaagcataa
      841 cgtgactata aaaccaacta aaaattatca taatcatctg acccgtatcg ctacgttttg
      901 cgcgctgctg tattgcaata gtgctttctg tgctgaactt gttgaatatg accatacctt
      961 cctgatgggg cagaatgcct caaatattga tctcagccgg tatagtgaag gtaaccctgc
     1021 tataccgggt atgtacgacg tcagtgttta tgtcaacgat caacctatta ttaaccaaag
     1081 cattacattt attgaaattg aaggaaaaaa aacgcacaag cctgtatcac acttaagaat
     1141 ttattgcagt ttcatataaa ttctcccgat ataaataacg aaaaagccgt tctgcttgcc
     1201 agggatgaaa cactcggcaa ttgccttaat ttgacggaaa ttattcctca ggcttctgtt
     1261 cgttatgacg ttaacgagca acgtctggat atagacgttc ctcaggcatg ggtaatgaaa
     1321 aattaccaaa actatgttga tccatcgtta tgggaaaatg gcattaatgc ggccatgttg
     1381 tcatacaacc tcaacggata tcatagtgaa actcctggtc gaagaaatga cagcatttat
     1441 gctgctttta acggtgggat gaatttaggt gcatggcgac tgcgtgcctc aggcaactac
     1501 aactggatga ccgattctgg cagtaattat gattttaaga atcggtatat acagcgtgat
     1561 atcgcctcgc tgcgttctca actcattctt ggtgagtctt atacaacggg cgaaaccttt
     1621 gattctgtca gtatccgcgg cattcgttta tacagcgaca gccgcatgtt gcctccgaca
     1681 ttagcgagtt ttgcgcctat cattcatggc gttgctaata ccaacgccaa agtcac
//
LOCUS       DADLFA010000312         1715 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000312 DADLFA010000000
VERSION     DADLFA010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.314"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            122..1399
                     /locus_tag="JQH65_004048"
     CDS             122..1399
                     /locus_tag="JQH65_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY6141259.1"
                     /translation="MNKIKNYRWHMIALVCFITVINYLDRTALGIAAPTIMETTGITK
                     EQYSWIVSAFQLAYTLGQPVMGFFIDTVGLKLSFAICAAIWGLATMGHALTGTWSGLA
                     FMRALMGFSEASAIPAGVKTASTWFPAKERGVATGVFNMGTSLGAMLAPPLIAWCIMF
                     HSWQFAFIVSGSLALLAALFWFFCYKDPKDAKRLSDEERHYIESGQEQHLKTDKKEKT
                     SIKHILSQRNFWGIGIARFLADPAWGTINFWVPIFFVETLHFSLKEIAMFVWLPFLLG
                     DLGCLASGFVAKFFHDRGVSLINSRRITFTIAAVIMMTIGLVSIVENPYIAVLLISIG
                     AFSHQCLSTVAATLGGDLFKKDEVATAVGMAGACAWSGQLIFNLFIGAFVHIIGFAPF
                     FIALAFFDIIGAIALWTLIKVKDEEPQVQLATH"
BASE COUNT          447 a          368 c          372 g          528 t
ORIGIN      
        1 ttttaaaggg agaaatttac acacaataat tttcaatatt tcctctgtac ctgattttta
       61 ttacctatta cacggaaata ccaacaacca gggtttatta atataattaa aaggtttaaa
      121 catgaataaa ataaagaatt accggtggca tatgattgcc ctcgtatgct ttatcactgt
      181 aatcaattat ctggacagaa cggcgttagg tattgcggct ccaacgatta tggagacaac
      241 cggaataact aaagagcaat attcatggat tgtcagtgca ttccagttgg cctatacatt
      301 agggcaaccg gtaatgggct tctttattga taccgtgggt ctgaagttaa gttttgcgat
      361 atgtgccgca atttggggcc tggcgacaat gggccatgca ctaaccggaa cgtggtctgg
      421 tctggcattt atgcgcgccc tgatgggttt cagcgaagcg tcggcaattc cggcgggtgt
      481 aaaaaccgca tcaacatggt tcccggcaaa agagcgtggc gtggcgacag gtgttttcaa
      541 tatgggcacc tcactcggcg cgatgcttgc tccaccgttg attgcctggt gcattatgtt
      601 tcatagctgg caatttgcgt ttattgtttc aggtagtctt gctttgctcg cggctttatt
      661 ttggttcttt tgttataaag atccgaaaga tgccaaacgc ctttctgatg aagagcgcca
      721 ctatattgaa tcagggcaag aacagcatct taaaacagat aagaaagaaa aaacgtcaat
      781 caagcatatc ctcagccaac gtaatttctg ggggattggc atcgcgcgtt ttctcgcaga
      841 cccggcatgg ggaaccatta acttctgggt gccgattttc ttcgtcgaaa cgctgcattt
      901 tagcctgaaa gaaattgcca tgttcgtctg gctgcctttc ctgctgggcg atctcggctg
      961 tttagccagt ggttttgtcg cgaagttctt ccacgatcgc ggcgtgagtt taattaactc
     1021 acgaagaatt accttcacta ttgcagccgt cattatgatg acgattggcc tggtgagtat
     1081 tgtcgaaaat ccctacattg ccgtattact gattagtatt ggcgcgttct cgcatcaatg
     1141 tctttctact gtagcagcaa ctctgggtgg cgatctgttc aaaaaagacg aagtagctac
     1201 cgcagtgggt atggcaggag cctgtgcgtg gagcggtcag ttgattttca acctgttcat
     1261 cggggcattc gttcacatta tcggcttcgc gccgttcttt attgccctgg ctttctttga
     1321 cattattggt gctattgcgt tgtggacgct tatcaaagtt aaagatgaag aaccacaagt
     1381 acagttagct acccactaat tattttaaat aaaaacgcta taattgaggt tatggcgttt
     1441 ttaaagaagc ttgagttatc gaaccccgct taccaacgcg ttgtcagtca tctcccccct
     1501 ccctggtgca tcgttatatc cgtgatgagc gatttttgat attaatatct ttcaccatcc
     1561 gctattttca ccacttcaga aaacaaactt atttttatta atatcaattc tttttctttg
     1621 aacattcaac tgcgatattt gtaaaaacat taaacaggca acaataaaat gacattacca
     1681 acaactattt attcattccc cgcatattta cctcg
//
LOCUS       DADLFA010000313         1704 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000313 DADLFA010000000
VERSION     DADLFA010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.315"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(822..1061)
                     /locus_tag="JQH65_004049"
     CDS             complement(822..1061)
                     /locus_tag="JQH65_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase"
                     /protein_id="HAY6141260.1"
                     /translation="MPQGGVISPLLSNIILNEFDQYLNKRYLSGKARKDRWYWNHSIQ
                     RGRSTAVKENWQWKPAVAYCCYADDCVPRRRVLGT"
     gene            complement(1256..1613)
                     /locus_tag="JQH65_004050"
                     /pseudo
     CDS             complement(1256..1613)
                     /locus_tag="JQH65_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707076.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          379 a          475 c          412 g          438 t
ORIGIN      
        1 tcgtcaaaaa aaatcatctt cgtgaagttt tacgacatag tggaggagtt tgcttctttg
       61 aggtgcaggt tttcgtttgc gtggtttagc tgaacgggcc atataactac ctgaaagaca
      121 atgatattgt ctgtttttat aacggtaatc acaggccatg acaagccgca gccgtcaggc
      181 cgcctactcg ggtcggttta gaacgcttat ttagtaccag atcttactta taataaaggg
      241 gcttggcagt ttggataaac tattgtcctt attccactaa tacaaacttt ttgtaaagta
      301 tattaaatat acttcatttc atactgaaat ccccggaagt attcaatcaa acaattacac
      361 cataaaagga agagggggcc ccaattaagt tgtcaagcat gtttactgcg caatcgcttg
      421 gtgctccgtc cctgtacccc gcattggtac tctggcgctc atggggcttc cacttgcgct
      481 tctcccttca catcggggtg acaggtccac gttccccaca agagcctgaa tcgacttcac
      541 gccgctttta tgccggatgc cgcgcaggca gtaaacatat cccacaggaa cgactccacc
      601 attgttttga catcatctat atgctttcga cacatcatca gcggttcact ttcgttcgtc
      661 tcttcgaacc atacctgatc ggctcacgtc cgaccttttc ccatctcgct caccaccaca
      721 tctcttaaat gcagcagcag tggatggttt ggtgcctgcg cctgcacgcc gacaccgagg
      781 ggccttccct catctcttgt gccgcatggc tgcacaggga ctcacgtccc caggacgcgc
      841 cttcgtggca cacagtcatc ggcatagcaa cagtaagcaa cggcaggttt ccattgccag
      901 ttttctttga cagcagtact tcggcctcgc tggatactgt gattccagta ccagcgatcc
      961 tttctggctt ttccactcag gtagcgttta ttcagatact ggtcgaactc attcagtatg
     1021 atgttcgaca gcaacggcga gatcacaccg ccctgtggca tactttcact gaccgcccgg
     1081 aagagaccaa catcaacacg gcgttttcca cagcagtctc ataaaacgtg catcactgat
     1141 cctgcggcag acggctttca tcagcagccg gtgatgtact gtgtcgaagt agctggacag
     1201 gtcaccttca ataacccggc gccccgtgtt tccccgcagt cggtgagctg taatagtacg
     1261 tgatgtctcc cgcccacttc tggttcgggc cactggcgta aaaatcctgc gtcagcagat
     1321 tttctgatac aggcaggccg tgtgcacggt aactgatcgg gctgaacttc tgggaggctt
     1381 ttgcgctcag ttcctgacgg cgcagactgg ctgccacggt ttttacgtta aagtggtaac
     1441 cagagcacac agttcatccg tcaggcgtgg ggcaccgtaa cgctggactg ggtaaaagcc
     1501 ttgcggacaa cgctgtcgca gtgctgacgg aactgctgac gtgtgtttat ccttgtccgc
     1561 cgctgacacc acgtatacca gccgctgcgg gccacccgga gtacgcggca cattgccttg
     1621 atgacggttt acgcggtcgc ctgcggcaac ggctttccgg ctgacggtag tgtggacctt
     1681 tttaccccgg agaacaccgg caag
//
LOCUS       DADLFA010000314         1675 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000314 DADLFA010000000
VERSION     DADLFA010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.316"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            13..171
                     /locus_tag="JQH65_004051"
     CDS             13..171
                     /locus_tag="JQH65_004051"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141261.1"
                     /translation="MYLSRISEAISLSAGEVAHRLIVPSSDIDLGETELPVLGEITWQ
                     AYDPARSK"
     gene            171..761
                     /locus_tag="JQH65_004052"
     CDS             171..761
                     /locus_tag="JQH65_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HAY6141262.1"
                     /translation="MDSLQDDYTKLLYGLMQPGPAWSDTDGVLDGLAPSLVRVHQRAD
                     ELVIEIDPGQSTELIERYEELYGLPDSCSPVGTQTLRQRQQRLEAKANVAGGINEQFF
                     LDQLEALGYTGVTIEQFQHLDASPDPEWGDRWRYFWRVTLPVDAGAQWQTCTDACNTP
                     IRTWGDTVAECVINKLCPSHTVVLFSYPDEDEDAQD"
     gene            748..1413
                     /locus_tag="JQH65_004053"
     CDS             748..1413
                     /locus_tag="JQH65_004053"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY6141263.1"
                     /translation="MHRIDTLTAVKDKFGPGKNGFTDGNLRTGRLATWLNSAMWNAIQ
                     EEICGVIEKAGIELNKEEHDQLYKAILLLVGGAINEEALLIKNNLSDVEDRDEAVENL
                     GLKPTVDKAKNAVQRDGDTMTGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSEG
                     QGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGIQLLSGIT
                     IQV"
     gene            1434..>1675
                     /locus_tag="JQH65_004054"
     CDS             1434..>1675
                     /locus_tag="JQH65_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY6141264.1"
                     /translation="MDVYANGQHVFRFQNGVAIALKNIQAGNAKKFTLSSANNSTKNA
                     AFYLWGNPSRPVVAELGDDSGWHFYSQRNTDGSITFA"
BASE COUNT          423 a          361 c          483 g          408 t
ORIGIN      
        1 ccagaggggc gtatgtatct gtccagaatc agtgaggcca tcagtttgtc tgctggtgaa
       61 gtggcccacc ggttaatcgt cccttcatcg gatattgacc tgggggagac cgagcttccg
      121 gtgctcggcg agatcacctg gcaggcttat gacccggcaa ggagtaaata atggattcgt
      181 tacaggatga ttatacaaag ctgctgtacg gactgatgca gccggggcct gcatggtcag
      241 ataccgacgg tgtacttgac ggtctggcac catcgcttgt gcgtgttcac cagcgggctg
      301 atgaactggt gattgaaatc gatcccggtc agtccacaga gctgattgaa cgttatgaag
      361 aattgtatgg tttacctgat tcctgttccc ctgtcggaac ccagacatta cgccagcgtc
      421 agcaacgtct tgaagcaaaa gccaatgtgg ctggtggcat aaatgagcag ttcttcctgg
      481 atcagcttga ggcgctggga tataccggcg taacgatcga acagttccag cacctggatg
      541 caagccccga tccggaatgg ggagatcgct ggcgttattt ctggcgtgtg acgttgccgg
      601 tggatgccgg tgctcagtgg cagacatgca cggacgcctg caacacaccg atccggacgt
      661 ggggggatac ggttgcggag tgcgtgatta ataaattatg tccgtcacac accgtcgttt
      721 tattttccta tccagatgag gatgaagatg cacaggattg atacgctgac tgcggtaaaa
      781 gataagtttg gtccgggtaa gaacggattt actgacggaa atcttcgcac aggacgtctt
      841 gctacctggc tgaacagtgc catgtggaat gccattcagg aggaaatctg tggtgtcatt
      901 gagaaggccg ggatagaact gaataaagaa gaacacgacc agctatataa ggctatatta
      961 ttgctggtgg gcggtgcaat taacgaagag gcattgctga taaaaaataa cctttcggat
     1021 gtggaagaca gggatgaggc tgttgaaaac ctcggattaa aacccacggt ggataaggca
     1081 aaaaatgccg ttcagcgtga tggcgacacc atgaccggag agctgaaaat ccgtggtgtt
     1141 aatgcgctga ggattttcaa cgaagctttt ggcctgattt ttcgtcgttc ggaagagtgc
     1201 ctgcacctta ttcccaccag tgaaggtcag ggggaaaatg gcgatattgg tcccctgcgc
     1261 ccgttcacca ttaatctgcg gacgggtgaa atatccatgt cgcataaagt gtctgttggc
     1321 ggtggttcgc aggtcaatgg tgcgctgggt atcggcgttc agaacgccct ggggggaatt
     1381 caattgcttt cggggataac gatacaggta taaaacaaaa cggcgacggc attctggatg
     1441 tttatgcgaa tggacagcat gtatttcgtt tccagaatgg tgtggcgata gcgttaaaaa
     1501 atattcaggc cggaaatgct aaaaaattca cgttatccag cgccaacaac tccacgaaaa
     1561 atgcagcgtt ttatttgtgg ggtaatccat ccaggcctgt tgttgcagag cttggtgatg
     1621 attccggctg gcatttctac agccagagaa ataccgatgg tagtatcaca ttcgc
//
LOCUS       DADLFA010000315         1668 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000315 DADLFA010000000
VERSION     DADLFA010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.317"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            15..1270
                     /locus_tag="JQH65_004055"
                     /pseudo
     CDS             15..1270
                     /locus_tag="JQH65_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000535347.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
     gene            1563..>1668
                     /locus_tag="JQH65_004056"
     CDS             1563..>1668
                     /locus_tag="JQH65_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine acid phosphatase"
                     /protein_id="HAY6141265.1"
                     /translation="MNKTLIAAAVAGIVLLASNAQAQTVPEGYQLQQVL"
BASE COUNT          547 a          262 c          341 g          518 t
ORIGIN      
        1 tatagacgct atatatgggt agtaatatac atggaattag ttgcactgca aataattatt
       61 tgaaacaggc ctggaacgat ataaaaaatg agtacgaaaa aaatcaaaca tattcaatca
      121 cgctttttga aaacacactg gtgtgtttta tgcggttata caatgaactc agacgtaaag
      181 taaatgaaga ggatactcca tgtctggaat gtgaatcact agaaaaagaa tttgaggaaa
      241 tgcagaatga taatgatcta tcattattta tgagaacatt gcgtactaat gatacacaaa
      301 tttattcagg gtttcaggag gtattacata tactatacaa tatgttcaaa atgttgatat
      361 tgttagagtg tccttgccgg gcagaggttc agagtctatc acagatttta aaggttatta
      421 ttggtatggc tttatggaat atattgaaaa cattactgcg tgtgatgatg ttttttctga
      481 gtattgttta gatgatgaaa atatgagtat ccagccagaa cagataaata cgccgggaat
      541 atctgattta gatactggta ttgatttgtc tggcatatct tttattcaga gtgaaattaa
      601 taaaacatat ggattaaaat acgctccagt agatggcgat ggatattgtc tgttaagagc
      661 tatactggtt ttaaaagaac atgagtattc atgggcgctg ggcggccata agacgcaaaa
      721 gcaagtatac gaagagttta ttaaaattgt tgataaacaa acgatcgagg ctcttgttga
      781 tacggcattc tataatctca gggaagatgt aaagacgtta tttggcgtta atctacaatc
      841 tgacaacaaa attcaggggc agggtggttt tttatcatgg agttttttgt cttttaaaaa
      901 agaatttatt gatagttgct tgaatgacaa aaaatgcata ctgcatttac ccgaatttat
      961 atttaatgat aacaaggtac gccttgtttt agacaccgac ccagagcaga gggttaatga
     1021 ggtgaaaaat ttcctcactg ctctttcaga tagcatttgc tcattattta ttgttaatag
     1081 taatatggca tcaatctcct tggggaatga atccttttca acagatgacg accttgagta
     1141 tgtttattta ataaacactg gcaatcatta tgatgtttac ctccctcctg aactttttgc
     1201 tcaggcttat aagttaaaaa ataaggaaat gaatgcgcaa ctcgactatt taaatcgtta
     1261 tgcaatttaa tggcaaaggc atatgctaaa aaccattgtt attagtctca cactttttta
     1321 ttggtaaata ttgtctctgt attggtaacg ccgcagatat tctgtttagc cacaggtgca
     1381 attatcagcg gcgtacgcga ggcaggggct aatcaggcat agtttgcgtc aaaccttgcc
     1441 tgtttttgaa gatgtatata gaaaaacagg cgttcaacaa gccattttgc gaacctgttc
     1501 ccggaaaaaa gtcatatttc tgtcacactc tttagtgatt gataacaaaa gaggtgccag
     1561 gaatgaacaa aacgctaatc gccgcagctg tggcagggat agttttactc gcttcaaacg
     1621 ctcaggcaca aaccgtaccg gaaggctatc agctacagca agtgctca
//
LOCUS       DADLFA010000316         1648 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000316 DADLFA010000000
VERSION     DADLFA010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.318"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..950)
                     /gene="corA"
                     /locus_tag="JQH65_004057"
     CDS             complement(<1..950)
                     /gene="corA"
                     /locus_tag="JQH65_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HAY6141266.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            1320..>1648
                     /locus_tag="JQH65_004058"
     CDS             1320..>1648
                     /locus_tag="JQH65_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141267.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFSVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQ"
BASE COUNT          426 a          427 c          412 g          383 t
ORIGIN      
        1 tacaaccagt tcttccgctt aaagtacaga tacggtgcca ggcccgcgag gatcataaag
       61 ataatcgcgc cagggtagcc gaagctccac ttcagttctg gcataaactc aaagttcatg
      121 ccatagctgg aagcaacgag cgtcggcggc aggaatacca cggataccac cgagaagatt
      181 ttgatgatgc ggttctgctc gatgttgata aaacccatcg ccgcctgcat caggaagttc
      241 accttctgga acagagattc gttatgcggc agcagggatt cgatatctcg caggatttca
      301 cgcgcctgct ccagttgtcc acccggtaag cgtgctttac gtaccaggaa gttgagcgcg
      361 cgctgggtat ccatcagaca caggcgtact ttccagccga tatcttccag ttccgccagc
      421 gtggagagcg cctcatcgta ctcatcgccc tgatgccctt ccataatcac ccggctcaac
      481 tgctccaggt cgctatagat attttcaatt tcatctgcca actgttcgat tttggtttcg
      541 aacagatcca gcagcagctc gtaggcgtta ccgtctacca tcgactggct acgggcacgc
      601 atacgataca gacgaaaagc gggcagttca cgctcacgca gagtaaacag acgaccatca
      661 cggatggtaa atgccacagt ggagttaccg gcgtgatctt ccgcatcttc aaagaagaag
      721 aaggagtgaa tatgcaggcc gtcgtcgtct tcaaagaaac gtgccgatgc ttcgatgtct
      781 tccagttccg ggcgggttgc caggctctgg ccaagttcag attgtacacg cagtcgctcg
      841 tcgtcgtccg gttcgacaag atcaatccat actgcattta caaggggttg tgactcttcg
      901 acttccagcc gggtcagtcg gttattttcc agttgaaatg cgctcagcat gaccgggact
      961 cccaatgctt aaaatatcgg acagttcggt gggcacacag aaacaaattg ggtttcagac
     1021 cattaaacag cctgactcag cgcgacggga aaaattgagg tcgctgacaa ccgctaaggc
     1081 tatcagcaaa aagggatagc cttaggagtt gatcctggat gacaggatag tgagccagta
     1141 tctactgggt gtgtccaagg cgaatgtcct cttagaatga tcgtgcgcgc atgttacgcc
     1201 agcaaaattt tgccgtcaac acgcaacaga acaccacaga acaatagttg aacttcgcgc
     1261 tttttggtac tcctcaaggt cgctaacagg ataaaaaaat cccacatcca ggacctataa
     1321 tggcgcatcg gctactcatt ggtaaaggaa tgatcacctt gaatctcaaa cggatttttc
     1381 tcaccctcac cttacttccc ttgttttctg ttgccgctga tgattgcgca ctctcagatc
     1441 cgacgctgac cgtacaggcg tataccgtta atcctcaaac agagcgggtg aaaatgtact
     1501 ggcaaaaagc caatggcgaa gcgtggggaa cgttacatgc tctgctggcg gatattaata
     1561 gtcagggtca ggtgcagatg gcgatgaacg gcggcatcta tgatgaaagc tatgcgccgc
     1621 tcggtttgta catcgaaaac ggtcagca
//
LOCUS       DADLFA010000317         1626 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.319, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000317 DADLFA010000000
VERSION     DADLFA010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.319"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..543
                     /locus_tag="JQH65_004059"
     CDS             <1..543
                     /locus_tag="JQH65_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HAY6141268.1"
                     /translation="QIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLYYLNQ
                     HFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAANELT
                     SALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISDLKQI
                     VPRSAESQQRDKVATFPKLA"
     gene            complement(550..978)
                     /gene="uspC"
                     /locus_tag="JQH65_004060"
     CDS             complement(550..978)
                     /gene="uspC"
                     /locus_tag="JQH65_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HAY6141269.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
BASE COUNT          466 a          331 c          351 g          478 t
ORIGIN      
        1 cagattgcac caacgtcgcg tggtgatgtg caagcctatc aggatattcg tcatcagctc
       61 gaaaatgaag ctggacgaat taatggtaaa tacgggcaat taggctggac gccgctttat
      121 tatttgaatc agcattttga ccgtaaatta ctgatgaaaa tattccgcta ctctgacgtg
      181 ggcttagtga cgccactgcg tgacgggatg aacctggtag caaaagagta tgttgctgct
      241 caggacccag ccaacccggg cgttcttgtt ctttcgcaat ttgcgggagc ggcaaacgag
      301 ttaacgtcgg cgttaattgt taacccctac gatcgtgacg aagttgcagc tgcgctggat
      361 cgtgcattga ctatgtcgct ggcggaacgt atttcccgtc atgcagaaat gctggacgtt
      421 atcgtgaaaa acgatattaa ccactggcag gagtgcttca ttagcgacct aaagcagata
      481 gttccgcgaa gcgcggaaag ccagcagcgc gataaagttg ctacctttcc aaagcttgcg
      541 taggagcggt taatcacccg taagtggaac taacagcaca tcgacctcac ttgaggcaat
      601 cactcttttc gccgagcagg atgctcgcga aaagaaactg tgattgtgat taccgcaaat
      661 caccaaatcg aaatgatgtt tgtgacatac ttccagaata tgttcgctta actcaccgta
      721 ggcaataaac gtcttgtcaa cgggataccc cgcatcctga attaacttat caagaaagct
      781 ttgcgtttct tcttgcatca cgctgcgtaa atcttcaagc atcggcgcgg ctaattgatt
      841 gtacatttcc gggtcggaag cgagagtaat taaactgatg tgtcccttta ctggcctggc
      901 gatagatacc gcttttgcca gcagttgctg actttccggt gtaaccgcaa cagcgacaag
      961 aatattgcta tagctcataa cctgttcctt attctgtgaa cttcaggtga cattaaagct
     1021 ttactcctaa tggagaaacg acgcaatccc aactcggtca aacaggtaaa tattagtctg
     1081 attattagta aagacaatta atcaatcact cccggtgatc atataatttc aaatgtaatt
     1141 ataagaaaat agtgtactga ttctccgcat taaacaagtg gttaatatat gtaaaacatt
     1201 tcattagcat taagttgatt gttgcctttc tttgtattta attagtttgt tgtgcggtaa
     1261 gtgtctgttt aaaaatagct tatctggtat tgcatgaaag tgattattta tagcagatga
     1321 ttatttacgg tgagttattt taactgtgcg caacatccca tttcgattat tccagtttca
     1381 tttttgcttg ctagcgtagc gaaaaacttt ttaacagatt gaaatacacc caaaacaaaa
     1441 gtatggctta tacatttatg ttaagtaatt gagtgttttg tgtgatctgc atcacacatt
     1501 attgaaaatc gcagcccccc tccgttgtat gtgcgtgtag tgacgagtac agttgcgtcg
     1561 atttaggaaa aatcttagat aagtgtaaag acccatttct atttgtaagg acatattaaa
     1621 ccaaaa
//
LOCUS       DADLFA010000318         1621 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.320, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000318 DADLFA010000000
VERSION     DADLFA010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.320"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(1..1577)
                     /locus_tag="JQH65_004061"
                     /pseudo
     CDS             complement(1..1577)
                     /locus_tag="JQH65_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433765.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          420 a          411 c          349 g          441 t
ORIGIN      
        1 caaaaaggca aacagatccg aagcgggata atctttttgg ggagaattgt tccagttagc
       61 aatatatccc gacagggggt tatacacctt agggttcatt tcaaaaggca atagcccttt
      121 ccagtcccat tttcccgtac caggaacggg taatcgcgga tcatggcctg attgacgatc
      181 tggataagca ccagtatgaa cataaccaat attgccgttt acatcagcat agtaccagtt
      241 gatggtcagt gcttgtttcg ctactcctgc caatttttgg ccttcatctg atgagtccag
      301 gccagcaaag acgccacctc tttaccatcc catgcgcggg atttagcgta agccgtttgt
      361 gtcgtctggt cagtttggag aatgttgcca tgcaccgtac gccagacagt aaaggtctct
      421 gcctgaccat ttttcaccgt aatggtttcc tcacggctta acattttcac ccacttacaa
      481 ttatgtaagt agtagcctgg tttctctgcc gacagccgtt cagcaaaaat atcgacatca
      541 tcgccgaaac ctgccgttga tccccaggaa atcacaccat tatgaccaaa aaccagccca
      601 ggataggcaa atggtgtatt gccagtgaca tcataaccag caccgtgcag accaatacca
      661 taagtatacg caggcgcata ccagccaaac tgcggaccat ttaccatgat tgctttcgca
      721 tcctgggctt tgcttttgcc gatcacccac atattgctgg tcgttggata ccccgccaga
      781 ccattggcac caccctgtgc aaattgtgca gcaatagttt cccggttctt ccctgctgtt
      841 aacgccagca gtgcgccatc cgcccctttt gctggtcggt caagcattgg tgcaggtaaa
      901 tcgtagcgtg gcaacagagc tgctgtttgc gagttttgct gattaaattt aagtgggtag
      961 ctactctctt gtacggcaat agtggttggc gctgatgggt ttaccagcca tttcaactga
     1021 ttaaataccg ccatgccttg tgatacacca tatttatctt ttaaagccgt tagcagtgcc
     1081 agattatcaa tttcgctagt gctatcagag aagcggtttg ccatggtgcc cacaaatatc
     1141 atcgcgacat caaacggttc ccagcgctta ggagtaaagc caaatgtatt aaactgtttt
     1201 ggtaagagcg tctctggatt ggtatttacc ttatcaatcc aggcattcat tccatcagcg
     1261 tagccttgca gaatggacat atcctctggg gaaagggcat caatttgcgc ccggatagca
     1321 tccggccagt agttacgacg gatatcttta tcaaatttca caaaatcttt gccaagcact
     1381 tccgcgacag tcccttgagt actgcgacgt gccatttcca tctgaaaaag gcgatcttgt
     1441 gctactacat agccatagcc ataaaatagg tgccatgtat cattggcata aatatgcggc
     1501 atgccgtatt catcgcgaac aatctttatc tcacttgacg actgctcagc cagtgcaggt
     1561 aagctccaat aatacatcag ggaagcagta acacagttca cgatcatacg atttctattt
     1621 t
//
LOCUS       DADLFA010000319         1583 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.321, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000319 DADLFA010000000
VERSION     DADLFA010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.321"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(69..491)
                     /locus_tag="JQH65_004062"
     CDS             complement(69..491)
                     /locus_tag="JQH65_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141270.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELPLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSSDDSVVVH"
     gene            complement(475..702)
                     /locus_tag="JQH65_004063"
     CDS             complement(475..702)
                     /locus_tag="JQH65_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY6141271.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            779..1186
                     /locus_tag="JQH65_004064"
     CDS             779..1186
                     /locus_tag="JQH65_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY6141272.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1389..1541
                     /locus_tag="JQH65_004065"
     CDS             1389..1541
                     /locus_tag="JQH65_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 domain-containing protein"
                     /protein_id="HAY6141273.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LAKNTS"
BASE COUNT          445 a          412 c          327 g          399 t
ORIGIN      
        1 cgaaccttcc tccggatata atctgtgatt ccccaatcaa cagaaccaga ggaggttcgg
       61 cataaacatc aatgcacaac gacagaatcg tcggacgacc caccaccgct gaaatgtgct
      121 ttccggtaaa cggcctgaac tgcgtcatca tgcgcatcaa ttgccgtact caacgcttcc
      181 tgcgccgcca gtaatgcacg gcgttccagg gtatcgaaga tacagagtcg gtgacgcagc
      241 tcacgcggaa gaattgccag aaccgcaggg atcagtttct gaattttctc cctttgcgct
      301 ttcgtttcac ctttcaacca acggtgatag atattctgct gattgttcca gtccttgcct
      361 ggtaccagag gcaattcgcc gcccccctgc cgcagatatt cttcagtaat tgcgttagcg
      421 acccacgcct gccctttttc ggctgccagg gctaacagca ctgattcgat gtgctcatgc
      481 ctgattttca tgaatcacct tcccaggtcg ttttgcctta cgatattcgt cataaacttt
      541 ggggtcgtac tgaagttccc cgccagatgc ctcttgcagg cgcatcgcgc ggccttcagg
      601 aaccagttcc ccccatgcag caatgcttgc cagcctaact cctgcggcat tggcaagctt
      661 tgttttgctg ccaaaaaacg ctatagcatc aattttcaac atatcgagct ccttagattt
      721 tcctaaggaa actagatcgt agagaaacct aagtcaagaa agattagaat tccctaatat
      781 gaaaaacgaa accttcggtg ctcgcctctt acataggcgt aaaaaattaa aactgtctca
      841 ggccgcatta ggtaagctgg tcaaagtggc tcacgtaaca atttctcaat gggaaagaga
      901 tgaaacacag ccggcgggga aaagattatt cgcactgagc caagcgcttc agtgctcacc
      961 gacttggctt ctttttgggg atgaagataa acaaccaggc gaaccgatcc cggataatca
     1021 gccagccatt ctgacagaag atcaaaaaga gttacttcaa ctgttcgacg cactgcctga
     1081 gtcagagcaa aaggccctgt tgtcagagat gcgtgctcga gttgaaaatt tcaacaaact
     1141 ttttgaagaa ctactcaaag ctcgcaaaag aagcgcaaac aaataatccc cttttttctc
     1201 cacaccctgt aataaaaagc acaaactttc aaacacttgt gttttttaca tcaaaaaaac
     1261 ttaggttttt atacataaaa tcttgactcc atgtcttagg ttattctaaa tttcattcat
     1321 caagacaccg cacggtgttc tcagcaaaca gttccactac cccggcgtta aggggaaatg
     1381 aggtcagcat ggatactatc gatcttggca acaacgaatc cctagtgtgt ggcgtgtttc
     1441 ccaatcagga cggcacattc accgccatga cgtataccaa aagcaaaacg tttaaaaccg
     1501 aagctggcgc acgtcgctgg cttgccaaaa ataccagcta atccatttat tggattaatt
     1561 caatattctc gctgtagagg tat
//
LOCUS       DADLFA010000320         1572 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000320 DADLFA010000000
VERSION     DADLFA010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.322"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..233
                     /locus_tag="JQH65_004066"
     CDS             <1..233
                     /locus_tag="JQH65_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HAY6141274.1"
                     /translation="FFVQFIDVNAPHTGISFFILATTLELVSFCYLSFLIISGAFVTQ
                     YIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            406..766
                     /gene="yeaR"
                     /locus_tag="JQH65_004067"
                     /pseudo
     CDS             406..766
                     /gene="yeaR"
                     /locus_tag="JQH65_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
     gene            770..952
                     /locus_tag="JQH65_004068"
     CDS             770..952
                     /locus_tag="JQH65_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HAY6141275.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            1100..1348
                     /locus_tag="JQH65_004069"
     CDS             1100..1348
                     /locus_tag="JQH65_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAY6141276.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(1407..1502)
                     /gene="yoaJ"
                     /locus_tag="JQH65_004070"
     CDS             complement(1407..1502)
                     /gene="yoaJ"
                     /locus_tag="JQH65_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HAY6141277.1"
                     /translation="MLPRGHKDEKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(1483..>1572)
                     /gene="yoaK"
                     /locus_tag="JQH65_004071"
     CDS             complement(1483..>1572)
                     /gene="yoaK"
                     /locus_tag="JQH65_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HAY6141278.1"
                     /translation="GIIFPVVIFITAVVFLAWFFIGGYAAPGA"
BASE COUNT          417 a          350 c          374 g          431 t
ORIGIN      
        1 cgtttttcgt acagtttatc gatgttaatg ccccacatac gggaatttca ttctttattc
       61 tggcgacgac gctggaactg gtgagtttct gctatttgag cttcctgatt atttctggtg
      121 cttttgtcac gcagtacata cgtaccaaaa agaaactggc taaagttggc aactcactga
      181 ttggtttgat gttcgtgggt ttcgccgccc gactggcgac gctgcaatcc tgatacattc
      241 agcccgcgtt gtcgcgggct tcccacttgt aatcctccct gatttttctc tgatatggtg
      301 ctaaaaagta accaataaat ggtatttaaa atgcaaatta tcaggcgtac cctgaaacgg
      361 ctggaataaa ccgttttcag cgcattcacc gaaggaggga aaaggatgct gcaaatccca
      421 cagaattata ttcatacgcg ctcaacgcct ttctggaata aacaaactgc gcctgccgga
      481 ataattcgaa cgtcatcttg ataaaggaac gcgccagggg ggttacccac gcctttccgt
      541 tatgcatggg gcggtcaaat atctcggcta cgctgatgaa cacagtgcag agcctgatca
      601 ggtgatcctt atcgaagcgg ggcagtttgc ggtgttccct ccagaaaagt ggcacaacat
      661 tgaagccatg actgacgata cttatgtcaa cattgacttc ttcgtggctc ctgaagtcct
      721 gatggaaggt gcgcaacaac ggaaagtcat tcataacggg aaatgaatca tgggcaaagc
      781 aacgtatacc gtgaccgtca ccaataacag taatggcgtt tctgtcgatt atgaaacaga
      841 gacgccgatg actttgctgg tgccagaagt ggcggctgaa gtgataaaag atctggtgaa
      901 taccgtgcgt tcttatgaca cggaaaacga acatgatgtt tgtggttggt aattatcact
      961 gatcgttgat ggcgcaattt ctgcgccatc gcaaaaaaat cctccgtgat gctcgtcaca
     1021 aatcttgccc tcccgatgtt gtgctatgct ttttatcagc gactaacccg ctaatgcaaa
     1081 acagataagt gaggaaacaa tgggcattct gtcatggatt atttttgggc ttattgccgg
     1141 tattctggcg aagtggatca tgccaggtaa agatggtggt ggattcttta tgactatcct
     1201 gctggggata gtcggtgccg tagtcggcgg atggatcagc acgctgtttg gctttggtaa
     1261 agtcgatggc ttcaattttg gcagcttcgt ggttgccgtt attggtgcga ttgtcgtgct
     1321 atttatctac aggaagatta aaagttaacg cttaaattgc acaaaggctg cacacaggca
     1381 gcctttgcta ttttttagag gtgacgttac caccatccca gctcagtgcc gagtacgaca
     1441 ataatcgcca cacccatcat aataatcgtt gttttttcat ctttatgccc ccggggcagc
     1501 atagccgcca ataaaaaacc atgctaaaaa tacgaccgct gtaataaaga ttacaaccgg
     1561 aaaaataata cc
//
LOCUS       DADLFA010000321         1551 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.323, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000321 DADLFA010000000
VERSION     DADLFA010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.323"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..409
                     /gene="bluF"
                     /locus_tag="JQH65_004072"
     CDS             <1..409
                     /gene="bluF"
                     /locus_tag="JQH65_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HAY6141279.1"
                     /translation="PEQIIVEFTESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFA
                     GLLLLSRFQPDRIKISQELITNVHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEW
                     MWLESAGIEMFQGDLFAKAKLNGIPSIAWPEKK"
     gene            614..1345
                     /locus_tag="JQH65_004073"
     CDS             614..1345
                     /locus_tag="JQH65_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAY6141280.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
BASE COUNT          448 a          335 c          354 g          414 t
ORIGIN      
        1 gcctgaacaa atcatcgttg aatttactga aagtgaagtc atatctcggt ttgatgagtt
       61 tgccgaagcg attaaatcgc taaaggctgc tggtatcagt gtagcaattg atcattttgg
      121 cgcaggtttt gctggtttgt tactcctgtc acgcttccag cctgacagaa ttaaaatcag
      181 tcaggaattg attaccaatg ttcataaaag tgggccacgg caggcaatta ttcaggcgat
      241 cataaaatgc tgtacatcac ttgaaattca agtcagtgct atgggcgtgg caacaccaga
      301 agagtggatg tggcttgaat ccgcaggaat tgagatgttt cagggagatc tgtttgctaa
      361 agctaaattg aatggtatcc cttccatagc gtggccagag aaaaaataat tttcagcaca
      421 ttctttcaca tgatttcagt aaatcatcgt gaaaatgaat acgttacgtt taaatttaat
      481 ccacattaaa aataatgttt catccaattt agctgaaatc ttatttatac tttgtacaat
      541 tttagcgtat attgtacaaa gtattattgg gtcgtgtaca ggcgacggag atttgtgacc
      601 gcaaggagga attgtggctt attacagcat tggtgatgtt gctgaacgtt gcgggattaa
      661 tcctgtcact ctccgggcct ggcaacgccg ctacggtttg ttaaaaccac agcgcagtga
      721 aggcggacac cgactctttg atgaagaaga catacaacgc atcgaagaga tcaagcgttg
      781 gataagtaat ggcgtccctg taggcaaagt taaagcatta ctggaaacca ccagccagga
      841 tacggaagat gactggagcc gcctgcaaga agagatgatg tcaattcttc gcatggctaa
      901 tcctgccaaa ctacgcgcga gaattatttc actgggtcga gagtacccag tagatcaatt
      961 gattaatcat gtttaccttc ctgttcgcca gcgtctcgtg cttgatcaca acacctcccg
     1021 cattatgagc agtatgtttg acggcgcatt aattgaatac gcagcaacct cgcttttcga
     1081 aatgcgccgt aagcccggta aagaagccat tctgatggcg tggaatgttg aagagagagc
     1141 acgattgtgg ctggaagcat ggcgtttatc attgtcagga tggcacattt ctgttcttgc
     1201 tgatcccatt gaatcgccgc gcccggaact gttcccgacg caaacattga ttgtctggac
     1261 aggcatggca ccaacgagaa ggcaaaacga acttttacaa cactggggtg agcaaggtta
     1321 taaagtcatc tttcatgccc cctaatttca cctttatccc gccccttaga cagaatgtta
     1381 taatctgaac ccacaacagc atttgtaccg gagctgatcc gttgcaaaat gctcaccggg
     1441 agaattcagg taatccctct atcacactct gtacatatca cagccaaagg acagaccaga
     1501 ttgaagtggt aaaaccgatc gtgcacacta cgcagtctca caactaacaa g
//
LOCUS       DADLFA010000322         1522 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.324, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000322 DADLFA010000000
VERSION     DADLFA010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.324"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..1189)
                     /locus_tag="JQH65_004074"
     CDS             complement(<1..1189)
                     /locus_tag="JQH65_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HAY6141281.1"
                     /translation="MLREVSISNDTISVKLYRNEKIECACNFLMDKDSQGYIDLSDFD
                     VTNCHFKGDVISKVSFLSSNLQHVTFECKEIENCNFTKATVDNVIFKCRRLHNVIFLK
                     TSGECVDFSQDILDTVDFSQSQLGHSNFRECQIRNSNFDNCYLYASHFTRAEFLSAKE
                     ISFIKSNLTAVMFDHVRMSTGNFKDCITEQLELTIDYSDIFGNEDLDGYINNIIKMID
                     TLPDNAMILKSVLAVKLVMQLKILNIVNKNFIENMKKTFSHCPYIKDPIIRSYIHSGE
                     DNKFDDFMRQHRFSKVDFDTQQMIHFINRFNMNKGLIDKNNNFFIQLIDQALRSTDDM
                     IKANAWYLYKEWIRSDDVSPIFIETEEKLRTFNTNKLTRNDNIFILFSSVDDGPVMVV
                     SSQR"
     gene            complement(1192..1386)
                     /locus_tag="JQH65_004075"
     CDS             complement(1192..1386)
                     /locus_tag="JQH65_004075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141282.1"
                     /translation="MIGHITNSGGNNSLMKHDVVQGNNIVDLDLLRNFNGVTGLNRDN
                     FIYISDIFLNIKQRNEKIMQ"
BASE COUNT          520 a          266 c          225 g          511 t
ORIGIN      
        1 agcgctggga gcttaccacc ataacaggcc catcatcgac agaggagaac aggataaata
       61 tattatcgtt tcgtgttaat ttattagtgt taaaggttct taacttttct tcagtttcta
      121 taaatatagg tgaaacatca tcactacgaa tccactcttt ataaagatac caggcatttg
      181 ctttgatcat atcatccgtt gatcgtaagg cctgatcgat aagttggata aaaaaattgt
      241 tatttttatc aatcagccct ttattcatat taaacctgtt aataaaatgg atcatctgtt
      301 gggtatcgaa atccaccttg ctgaatcgat gttgacgcat aaaatcatcg aacttgttat
      361 cttcaccaga atggatataa ctgcgtataa ttggatcttt tatataagga caatggctaa
      421 atgttttctt catattctca ataaagtttt tattaacaat attaagaatt tttaattgca
      481 taaccagttt tactgccaga acggatttca atatcattgc attatctggc aatgtatcaa
      541 tcatttttat aatgttattg atataaccat cgagatcttc attcccaaat atatctgaat
      601 aatcaatagt taattccaat tgttctgtaa tgcaatcttt aaaattccct gtcgacattc
      661 gcacatgatc aaacataaca gctgtcaaat tcgatttaat aaatgatatt tctttggcag
      721 acagaaactc tgctctggtg aagtgcgaag cgtaaagata acaattatcg aagtttgaat
      781 ttctaatctg acattcgcga aaattactat gaccaagttg actctgcgag aagtcaactg
      841 tatcaagaat atcttggcta aaatcaacac attcgccact cgttttgaga aaaatcacat
      901 tgtgtaaacg tcgacattta aatatgacat tatcaactgt tgccttagta aaattgcaat
      961 tctcaatttc tttacattcg aaagttacat gttgtagatt tgatgataaa aaagacacct
     1021 tagaaataac gtcaccttta aaatgacaat ttgttacatc aaaatcagac aggtcgatat
     1081 acccctgcga atctttatcc atcagaaaat tacaagcgca ttctattttt tcatttctgt
     1141 agagttttac acttatagta tcattactaa ttgagacttc acgtaacata tttattgcat
     1201 gattttttcg ttccgttgtt ttatatttaa aaatatatca ctgatataaa taaagttatc
     1261 tctgtttaaa cctgtcaccc cattgaaatt acgtagtaaa tcaagatcta ctatattatt
     1321 tccttgaacc acatcatgtt tcatcaaaga gttattgcca ccagaatttg tgatatgacc
     1381 tatcataaaa cctctgtaat acagaaataa aataacctgg aaaagtagcc tgagcgtcca
     1441 gacactaccg gcgaaaggaa aagaatctct gataaggcat tgagataatg gacattctta
     1501 gtgaagaaga agtgaagtac ta
//
LOCUS       DADLFA010000323         1496 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.325, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000323 DADLFA010000000
VERSION     DADLFA010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1496
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.325"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..1494
                     /gene="pdeB"
                     /locus_tag="JQH65_004076"
     CDS             <1..1494
                     /gene="pdeB"
                     /locus_tag="JQH65_004076"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HAY6141283.1"
                     /translation="LPVGLSIWLAHQQVETSFIEELDTYSSRVAIRANKVATQGKDAL
                     QELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNVPQCSSLEHESPPDTFPEPG
                     KISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPASFIDVIPYSSWQIDAAIIGN
                     AHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILPFPEMNISIITWASTKMLQK
                     GWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQDAIENRDICVHYQPIVSLA
                     NGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPLTLLIIRSVFEDMGDWLRQH
                     PQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIALELTEREFADPKTSAPIISR
                     YREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVDALEYKNVTPHIIEMAKTLK
                     LKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDFLRWAEQHL"
BASE COUNT          396 a          351 c          367 g          382 t
ORIGIN      
        1 ctgcctgtcg gcttaagcat ctggctggcc catcagcagg tagaaacatc gtttattgaa
       61 gagctggata cctattcctc ccgcgtcgct attcgggcca ataaggtggc gacacaaggg
      121 aaagatgcgc tgcaagagct ggaaagatgg caaggcgctg cctgtagcga agcccatctc
      181 atggaaatgc gtcgggtatc ttacagttat cgctatattc aggaagtggt ttatatcgat
      241 aacaacgttc cccagtgttc gtctctggag catgaaagtc cgcccgatac cttccccgag
      301 ccaggtaaaa tttcgaaaga tggttatcgt gtctggttaa catcgcataa cgatttaggc
      361 attatccgtt acatggtcgc catgggaacg gcacattatg tcgtcatgat cgaccccgct
      421 tcctttattg atgtcattcc ctatagctca tggcaaattg atgccgccat tattggcaat
      481 gcccataacg ttgtcataac cagcagcgat gaaattgctc agggaattat taccaggcta
      541 caaaaaacac ccggtgagca tatcgaaaat aatggaatca tttacgatat cctgcccttc
      601 ccggagatga atatttcgat catcacctgg gcttcaacga aaatgttgca gaaaggctgg
      661 catcggcaag tctttatttg gttaccgctc gggttggtga ttggcctgct ggcagcgatg
      721 tttgtgctgc gtattttgcg ccgtatccag tcaccgcatc atcggctgca ggatgctatc
      781 gaaaatcgtg atatttgcgt gcactatcag ccgattgtct ccttagccaa tggcaaaatt
      841 gtcggtgctg aggcactggc gcgctggccg cagacagacg gtagttggtt gtcaccagat
      901 agttttattc cgctggcaca gcaaacaggc ctttctgagc cattgacgct actgattata
      961 agaagcgtct ttgaagatat gggcgactgg ctgcgtcagc atccacagca gcatatttcg
     1021 atcaatcttg aatccaccgt gctcacctcg gaaaaaatcc cgcaattgct gcgtgaaatg
     1081 atcaatcact atcaggttaa tcccagacag atcgcgcttg aactcactga acgcgagttt
     1141 gccgatccga aaaccagcgc cccgataatt tctcgctacc gggaggcggg ccatgaaatt
     1201 tatcttgatg attttggtac gggatattca agtttaagtt atttacagga tctggatgtc
     1261 gacattctga agatcgataa atctttcgtt gatgcgctgg aatataaaaa tgtcacgccg
     1321 catatcatcg aaatggcaaa aacactgaaa ctgaaaatgg tagcggaggg aatcgaaacc
     1381 agtaaacaag aagagtggct acgccagcat ggcgtgcact acggtcaggg ctggctctac
     1441 agcaaggcat taccgaaaga agatttctta cgctgggccg agcaacattt gtgaat
//
LOCUS       DADLFA010000324         1467 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.326, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000324 DADLFA010000000
VERSION     DADLFA010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.326"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..183)
                     /locus_tag="JQH65_004077"
     CDS             complement(<1..183)
                     /locus_tag="JQH65_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141284.1"
                     /translation="MLEDTTIHNAITDKALASYFRSSGNLLEEESAVLGQAVTNLMWI
                     CPYISRHLLSLNPLQAR"
     gene            complement(212..484)
                     /locus_tag="JQH65_004078"
     CDS             complement(212..484)
                     /locus_tag="JQH65_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HAY6141285.1"
                     /translation="MKTSDNERIKYEITGQVVLQILRMKINFSLQTLIKQLLVMKSAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATADNKSMFNGKKINRIH"
     gene            complement(527..763)
                     /locus_tag="JQH65_004079"
     CDS             complement(527..763)
                     /locus_tag="JQH65_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YcgZ"
                     /protein_id="HAY6141286.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANWPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEHATGEEEQKHYNALIGLLFE"
     gene            1077..>1467
                     /gene="bluF"
                     /locus_tag="JQH65_004080"
     CDS             1077..>1467
                     /gene="bluF"
                     /locus_tag="JQH65_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HAY6141287.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFR"
BASE COUNT          431 a          297 c          270 g          469 t
ORIGIN      
        1 gcgggcttgt aatgggttaa gtgataacag atgtctggaa atataggggc aaatccacat
       61 taaattggtg acagcctgcc ctaacactgc tgactcttct tctaacaaat tacccgaact
      121 gcgaaagtaa cttgctaacg ctttatcagt tattgcatta tgaattgtag tatcttcaag
      181 catggtaacc tctcatctta cttatgaaat tttaatgtat tctgtttatt ttcttaccat
      241 tgaacatact tttattgtca gctgtcgctg ttcttcggtt tggccctcct gaatcactat
      301 tgctaaaatc acgaattatg ctgtcaatta aatcgcgtcg gaaagcatct tcctcagccg
      361 atttcattac gagtaattgc ttaataagag tctgcaatga aaaatttatc ttcatgcgca
      421 gtatctggag caccacctgg ccagtaattt catattttat acgttcatta tcagatgtct
      481 tcataacgac ttcatagcta acctgttaaa taatcaggct gttgcattat tcaaaaagca
      541 acccaattag tgcattataa tgtttctgtt cctcttcccc agtcgcgtgt tccagtcggc
      601 aaagaagttt ggcacaaagc gactttcgac tcagattacg tccatctttc aaaatctcag
      661 tcacaatttc cccgagagtt tcctgctgag taggccagtt agcttttgca aagtaacgag
      721 tgattgctcc cgcagaatct aaagtcactg aattttgatg catgctacgc ctctgttaaa
      781 aatgttaatc aatgttaagt gaggtaacaa tagcaaactt gtacgaaata tgtgtacaga
      841 atgataaatt aattttttgc aattagtgcc aatacatata aaacaatgtg ttatgataaa
      901 acatattgca tcatttaact gacaatctat acgagatttt tcgccaacta gtgttcaact
      961 ttttccattt tttatcaatg taaagaaacg gttatgtttt ggtattaaaa gaaagacaca
     1021 agaagatatg tctgttaccg tcttactctc gcctcaccca ttaccctgga ttgttaatgc
     1081 ttaccaccct tatttatcgt agccatatac gtgacgacga acctgtcaaa aaaatcgaag
     1141 aaatggtttc gatagcaaat cgcaggaaca tgcagtctga cgtaacaggg atcttactgt
     1201 ttaatggttc tcattttttc cagcttctgg aaggtccgga agaacaggtt aaaatgatat
     1261 atcgggctat atgccaggat ccacggcact ataatattgt tgagctgctg tgcgattacg
     1321 cgcctgctcg ccgttttggc aaagcgggaa tggaattatt tgatttgcgc ctgcacgagc
     1381 gagatgacgt tttacaggcc gtattcgaca aaggcacatc aaaatttcag ctaacttatg
     1441 atgacagagc gctacaattt tttcgta
//
LOCUS       DADLFA010000325         1461 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.327, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000325 DADLFA010000000
VERSION     DADLFA010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1461
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.327"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..730)
                     /gene="pdeC"
                     /locus_tag="JQH65_004081"
     CDS             complement(<1..730)
                     /gene="pdeC"
                     /locus_tag="JQH65_004081"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HAY6141288.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHL"
     gene            1160..1441
                     /locus_tag="JQH65_004082"
     CDS             1160..1441
                     /locus_tag="JQH65_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141289.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT          422 a          322 c          324 g          393 t
ORIGIN      
        1 taagatgatt ataaaaatgg gttataagac gttgatatga agtcgcaaca atggctgcaa
       61 ttgggcgttt tgtcgaataa actgtcgcat ataaatagcc atttctttgt acggttaaat
      121 ccttcaaatg aatcagcgga gaaaacgttg ctgccgaggc ctctttgctt aacgagaaaa
      181 aggttttcgt caccgtatca tacacacccc attgcaatgt cggatcatca gacatcactt
      241 cactccagaa gagagggttg ataaccgcca cataatttcc ccgctgcata taggtcattt
      301 tatagccaga gaaaaaaggc gtatcgcggt aataatagat agaaacgtta ggttcacgct
      361 tataatcggc cggtgcaatc gtatagccgt ttacaggcgc tatcagcgat gagcataaaa
      421 aatggttatc acgggcatag atcaattcat taatataaag atagccacga ataatattca
      481 acattcgctt ttgatgggct ggagtgcaaa cttgcccctg atagcgttca gctgcatcgc
      541 tcactaaatc tgcctggcga atgaccagct cggatttatc cagtgccagt tgagcaaagg
      601 ttcggagctg attattcact tctgattttg cccaaaggaa ggcaatccat agcgaaagaa
      661 tgatgggaaa gagaactaaa aagataatgc ccggcaacgc cagtaattgg tgtcgtgcac
      721 gatgactcat tgctatcaat ttcctgcctg tttatacccg gccattgtaa ggtgaaaacc
      781 ctgggtaccg acgtctttag ataaatctta cggtataaat agcacttttg cctgatttga
      841 aattgatacc tttctaatcg tcacctggat gaaaataatt cattgatatt caaaattaat
      901 ttcctacagg aatgcagagg cggcgggaag atatgccctt aatgccactg aaaaaccaca
      961 taatctgtac agcaataaaa gtcacggcct aatatggcta acaaacaatc aattgtgata
     1021 tatttcacaa aattaatgaa acaaacagag tgtttcattt ttgtgttctg caagccccgc
     1081 gatagccaga gtgtaccacg cctcccgtga acaacgccgc gctgtccagg gcatcggctc
     1141 ttttcagagg aattgtttga tggctaccct caccactggc gtggttcttc ttcgctggca
     1201 acttcttagt gccgtaatga tgtttctggc cagcacactc aacatccgtt ttcgtcggtc
     1261 tgattatgtc gggcttgcgg tgatcagcag cggtctgggc gttgtttccg cctgttggtt
     1321 cgcaatgggg ttgcttggca tcacaatggc ggacatcacc gctatctggc acaacatcga
     1381 gtcggtgatg atagaagaga tgaatcagac accgccacaa tggccaatga ttttgacctg
     1441 atacgtagaa gcctccataa c
//
LOCUS       DADLFA010000326         1415 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.328, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000326 DADLFA010000000
VERSION     DADLFA010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.328"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..323
                     /locus_tag="JQH65_004083"
     CDS             <1..323
                     /locus_tag="JQH65_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HAY6141290.1"
                     /translation="APFYNGKSDTRTVDLSDAVYRRLILMKAMSNITDCSVPDINRML
                     RFMFGKKRRAYVLNNGGLRMSYIFESALSLAELAIIQSSGALPSPPGVYVSVVLKESR
                     NEGQ"
     gene            310..840
                     /locus_tag="JQH65_004084"
                     /pseudo
     CDS             310..840
                     /locus_tag="JQH65_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748478.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            1174..>1415
                     /locus_tag="JQH65_004085"
     CDS             1174..>1415
                     /locus_tag="JQH65_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY6141291.1"
                     /translation="MDVYANSQRVFRFQNGVAIAFKNIQAGTARKFTLSSANNSTKNA
                     AFYLWGNPSRSVVAELGDDSGWHFFSQRNTDNSITFA"
BASE COUNT          354 a          312 c          394 g          355 t
ORIGIN      
        1 aggcaccgtt ttacaacgga aaatcggata cccggactgt tgacctgtct gatgctgtat
       61 accggcggct gatactgatg aaagccatgt cgaacattac tgactgttcc gttccggata
      121 ttaaccggat gctgagattt atgttcggaa aaaaacggcg agcttatgtt ctgaataatg
      181 gtgggctgag gatgagttac atctttgagt ccgcgctctc gttggcagaa ctggcgatta
      241 tccagtcgtc gggtgcactg ccgtccccgc cgggtgttta tgtttcagta gttttaaagg
      301 agtcccgtaa tgaaggccag tgataaaccc cgccagctgg cggtcccctt tgcgagtacc
      361 ggagataaaa accgtatccc ggacaaggcg acacagcaga ccagagagag cggtaatgct
      421 gcgtatgatt caggttttcc tccggtgacc atgacagcgg tctcagcggg aggtataccg
      481 ccacacggca aggattttaa cggtctgatg tacgatatta ccgcagcaat acggttcgcc
      541 caggctggcg gtttgtacac gtataatgcc ggttttgcgg gggccattgg tggatatgca
      601 aaaggagcca ttctcgccgg agtcgcaaca acagcggtct ggctgaatac cacagacgat
      661 aacctgaccg atcctgaagg ctccgacagt gcgggctggg taaatcttct tgaggatccg
      721 aaaaggatat tcctgcggca gaagaacaat ctgtcagacc ttcagaataa agggacggta
      781 cgggataatc ttcaggttta cagtaaagag cagtcagatc aacgctatgt tcatcgggaa
      841 ggcgataaaa taaccggaga gctgaaaatc cgtggtgtta atgcgctgag gattttcaac
      901 gaagcttttg gcctgatttt tcgtcgttcg gaagagtgcc tgcaccttat tcccaccagt
      961 gaaggtcagg gggaaaatgg cgatattggt cccctgcgcc cgttcaccat taatttgcgg
     1021 acgggtgaaa tatccatgtc gcataaagtg tctgttggcg gtggttcgca ggtcaatggt
     1081 gcgctgggta tcggcgttca gaacgccctg ggggggaatt caattgttct tggtgataat
     1141 gacaccggat ttaaacagaa tggagacggt attctggatg tttatgctaa tagtcagcgt
     1201 gtattccgct ttcagaatgg agtggctatt gcttttaaga atattcaggc cggaactgcc
     1261 agaaaattca cgttatccag cgccaacaac tccacgaaaa atgcagcgtt ttatttgtgg
     1321 ggtaatccat ccaggtctgt tgttgcagag cttggcgatg attcaggctg gcattttttc
     1381 agccagagga atacagataa cagcatcacg ttcgc
//
LOCUS       DADLFA010000327         1350 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.329, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000327 DADLFA010000000
VERSION     DADLFA010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.329"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            220..1167
                     /locus_tag="JQH65_004086"
     CDS             220..1167
                     /locus_tag="JQH65_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HAY6141292.1"
                     /translation="MKNVLVTVPSFSARCVSASKLLRENNFNLIIKNNVEHLLKSEST
                     ALRESICAVIAGKDCYQADTLSLLPGVKIISRFGTGIDNIDLRAAQQSGIVVNNAVGI
                     NSNAVAEFIIGLIFASMRNIPGSYHAMQNGYWGESHGYELQGKRIGMVGYGNIGKTLA
                     KRLSGFDVELLAYDKQPDYQVADKVGVQFVSIEDIFMQSHVIIVLLPFSPELENFISH
                     KYLSMMRNGALIINAARGKLLDEGALLQVIEERNVFAALDVFSSEPLAQFSPLLHAKN
                     IITTPHIAAATVESYQQTGTHVAQSIIDYFAGREIKNVL"
     gene            complement(1246..1322)
                     /locus_tag="JQH65_004087"
     tRNA            complement(1246..1322)
                     /locus_tag="JQH65_004087"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1286..1288),aa:Met,seq:cat)
BASE COUNT          360 a          273 c          325 g          392 t
ORIGIN      
        1 aggtttagca ccgtcatgct cgtctactgt tgttctggtt gttggtgatg cggtggcgct
       61 tgcactctct gaattgaaaa aatttactcg tgctgatttc ggcttatatc atccaggagg
      121 tgcactcggc attaaagcaa attcataatt tcaaagtata aatacagtga ccggtcacgc
      181 catcttttgg ggtggcgttc attgaagtga gtgtcagcta tgaaaaatgt tctggtcacc
      241 gtccccagct tttcagcacg ctgtgtttcc gccagcaagt tgctaagaga gaataatttt
      301 aatcttatta ttaagaataa tgtggagcat ctgcttaaat cagaatccac tgcattacga
      361 gaatctattt gcgcggtcat cgccggaaaa gattgttatc aggcggacac gctatcattg
      421 ctgcctggtg tgaaaataat atcgagattc gggacgggga tagataatat tgatcttcgt
      481 gcagcacaac aatccggtat tgtagtcaat aatgcggttg ggattaattc aaatgctgtc
      541 gcggaattta tcattggact tatcttcgcg agcatgagaa atattcccgg cagctatcat
      601 gcgatgcaaa atggctactg gggtgagtcg catggctatg aattacaagg caaacggatt
      661 ggcatggttg gctacggcaa tataggtaaa accctggcaa aaaggttatc cggttttgat
      721 gttgagcttt tagcttatga caaacaaccc gattatcagg ttgctgacaa agttggagtt
      781 cagtttgtat caattgaaga tatctttatg cagtcgcatg tcatcattgt tcttttgcct
      841 ttttctcctg agctggagaa cttcattagt cataaatatt tgtcgatgat gcgcaatggt
      901 gctctaatca tcaatgccgc aaggggaaag ttgctggatg agggcgcttt actacaggtg
      961 attgaagaac gaaatgtgtt tgcggcgctg gatgtgttta gctctgaacc gttggcgcaa
     1021 tttagcccac tgctacatgc caaaaatatc attacaacgc cgcatattgc ggccgccacc
     1081 gttgagtcat accagcaaac cggtacacac gttgcccagt cgataattga ttacttcgca
     1141 ggaagggaga taaaaaacgt gttgtgagtt cagcgaacga agtttttaaa tacaaaaaaa
     1201 ccgcaatttg tggaacacat tgcggtttat cagcattcta agtattggtt gcgggggccg
     1261 gatttgaacc gacgaccttc gggttatgag cccgacgagc taccaggctg ctccaccccg
     1321 cgtcaccgta ctgctttatt tcatcaaatt
//
LOCUS       DADLFA010000328         1343 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.330, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000328 DADLFA010000000
VERSION     DADLFA010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.330"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..978
                     /locus_tag="JQH65_004088"
     CDS             <1..978
                     /locus_tag="JQH65_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAY6141293.1"
                     /translation="CEHLPLYRQSEIFARQGVELSRALLSNLVDACCQLMTPLNDALY
                     RYVMNSRKVHTDDTPVKVLAPGRKKAKTGYIWTYVRDDRNAGSPEPPAVWFAYSPDHQ
                     GKHPEQHLSPFRGILQADAFNGYDRLFSAEREGGALTEAGCWAHARRKVHDVYISTKS
                     ATAEEALKLIGELYAIEHEIRGLPVSERLAVRQMQSKPLLTSLYKLMQEKEHTLSKKC
                     RLRDAFRYIRKHWVALCNFSDDGLAEADNNAAERALRAVCLGKKNFMFFGSDHGGERG
                     ALLYGLIGTCRLNGIDPEAYLRYILSVLPEWPSNRVDELLPWNVALTNK"
     gene            1014..1130
                     /locus_tag="JQH65_004089"
     CDS             1014..1130
                     /locus_tag="JQH65_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase"
                     /protein_id="HAY6141294.1"
                     /translation="MQSKYRLSNRKAQRKLALVRKQYPKLFAHWYLLRASNE"
     gene            1204..1323
                     /locus_tag="JQH65_004090"
                     /pseudo
     CDS             1204..1323
                     /locus_tag="JQH65_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT          336 a          329 c          393 g          285 t
ORIGIN      
        1 tgcgaacacc tgccactgta tcgtcagagt gaaatttttg cccgtcaggg tgtcgaactg
       61 agccgtgcat tactctccaa cttggttgac gcgtgctgcc agttaatgac gccgctgaat
      121 gatgctctgt accgttatgt aatgaacagc cgcaaagttc acactgatga cacaccagta
      181 aaagtgctgg caccgggcag gaagaaggcg aaaacaggat atatctggac gtatgtccgg
      241 gatgacagga atgccggttc gccagagcct ccggcggtct ggttcgccta ctcaccggac
      301 catcagggta aacatccgga gcagcacctt agtcccttcc ggggtatcct gcaggcagat
      361 gcgtttaatg gttacgatcg gctgttcagt gccgaacgag aaggcggcgc gttgacggaa
      421 gcaggatgct gggctcatgc gcggcgcaaa gtccacgatg tatatatcag taccaaaagc
      481 gcgacagcgg aagaagccct gaaactaatc ggtgagctgt acgccatcga gcacgaaata
      541 cgcgggttgc cggtgtctga acgcctggcg gtcaggcaaa tgcagagtaa accgctactg
      601 acttccctgt ataagctgat gcaggagaaa gaacacacgt tatcgaaaaa atgccgtctg
      661 agagatgcgt tccggtatat caggaagcac tgggttgcgt tgtgcaactt cagtgatgat
      721 ggtctggctg aggcggataa taatgccgcg gaaagagcgc ttcgtgcagt ctgtctcgga
      781 aagaaaaact ttatgttctt cggcagcgat cacggtggag agcgtggtgc gctactgtac
      841 gggctgatcg gcacctgccg actgaacggt atcgatccgg aagcgtatct gcgctatatc
      901 ctgagcgtac tgccggaatg gccttccaac cgtgttgacg aactcctgcc atggaacgta
      961 gcactcacca ataaataagc gtcaatacgg tgctccgttg acgcttacag tggatgcagt
     1021 ctaaatacag actttcgaac cggaaggctc agcgaaagct ggcgctggta aggaaacagt
     1081 atccgaagct atttgcccac tggtatttac tacgtgcatc gaatgagtga tcaagagccg
     1141 tatgacgcga gagtgtcacg tacggttccg tgagcagccg gagagtgaaa ctctctccga
     1201 cggggttccg atagcggtgg cgaacatgcg gcggtgttgt actcgctgat cggcacatgc
     1261 cgtctgaaca atgtggagcc agaaaaatgg ctgcgttacg tcattgagca tatccaggac
     1321 tgaccggcaa accgggtacg cga
//
LOCUS       DADLFA010000329         1343 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.331, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000329 DADLFA010000000
VERSION     DADLFA010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.331"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            86..1005
                     /locus_tag="JQH65_004091"
                     /pseudo
     CDS             86..1005
                     /locus_tag="JQH65_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            1005..>1343
                     /locus_tag="JQH65_004092"
     CDS             1005..>1343
                     /locus_tag="JQH65_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY6141295.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVNAERIGVLG"
BASE COUNT          381 a          302 c          325 g          335 t
ORIGIN      
        1 tttagggtct aatcaactta atgatcaata tctatagtga tatcaccaac cgttaaaacg
       61 aaccgacaac tccatgagga acggaatgat gaataataaa gtcagcttca ctaacagcaa
      121 taatccaacc atctctttgt ctgcagtgat ctatttccct ccaaaatttg atgaaacccg
      181 tcagtatcag gcaattgtgg tatctcaccc aggtggggcg ttaaagaaca aacggccgga
      241 acctatgcca aaaaactggc ggaaaaggga tttgtcacta ttgcttatga cgcatcttat
      301 caaggtgaaa gtggcggcga gccgcgtcag ttagaaaacc catacattcg tactgaagac
      361 atcagtgccg tgattgatta cctgacaacg ctttcttatg tcgacaatac ccgaattggt
      421 gcgatgggaa tttgtgccgg tgcaggatat acagccaatg ctgcaattca ggatcgccgc
      481 atcaaggcca tcggtaccgt tagtgcggtc aatattggtt cgatgttccg taatggttgg
      541 gaaaacaatg tgaaatctat tgatgccctg ccatacgttg aagcgggttc aaacgcccga
      601 accagcgata ttagcagcgg agaatatgcc gttatgccac tggcaccaat gaaagagtcc
      661 gatgcgccga acgaagaact gcgtcaggcc tgggagtatt accacacccc tcgcgcgcag
      721 tatccaacag caccgggtta cgctactttg cgcagcctta accagattat tacctttgat
      781 gcttaccata tggcggaagt gtacctgact cagccgatgc agattgtggc gggcagccag
      841 gcagggagca aatggatgag tgacgatctg tacgatcgag catcaagcca agataaacgc
      901 tatcacattg ttgaaggcgc aaaccatatg gatctttatg acggcaaagc ctatgtggct
      961 gaagcaattt cagtattagc tccgttcttt gaggaaactc tgtaatgaaa catattcaaa
     1021 tccgcaatag tgatatggcc tggcatattg ctgccaatat tcagtttcca ccaaattttg
     1081 atgagtcaaa acagtatccg gcgattatca gcgtccatcc ttttggcagt tgtaaggagc
     1141 agacgtctgg caatatctat ggcaaagcat tggctgaaaa aggctatctc gttctcgctt
     1201 atgacgccag tttccaggga gaatctggtg gggagcctcg ctggatagaa gatccgacac
     1261 agcgcgtaga ggatattagc cgtgttatcg attatgcagt aactctgcca tatgttaatg
     1321 ctgaacgtat tggcgtgctg gga
//
LOCUS       DADLFA010000330         1333 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.332, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000330 DADLFA010000000
VERSION     DADLFA010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.332"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..173)
                     /locus_tag="JQH65_004093"
     CDS             complement(<1..173)
                     /locus_tag="JQH65_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141296.1"
                     /translation="MFDYQVSKHPHFDEACRAFALRHNLVQLAERAGMNVQILRNKLN
                     PAQPHLLTAPEIWL"
     gene            complement(206..427)
                     /locus_tag="JQH65_004094"
     CDS             complement(206..427)
                     /locus_tag="JQH65_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HAY6141297.1"
                     /translation="MRPNISITLTTPHVTIERYSELTGLSIDTINDMLADGRLIRHRL
                     RKDKKREKVMINIAAMTVDALSECNLNLN"
     gene            553..1122
                     /locus_tag="JQH65_004095"
     CDS             553..1122
                     /locus_tag="JQH65_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
                     /protein_id="HAY6141298.1"
                     /translation="MNLEKGGRGAIERMVEAYGFKTRQALCDHLGISKSTLATRYMRD
                     SFPAEWVIQCALETGTSLNWLTTGHGSKQTSGNTNTMEVAKYVLSDGALREDGFYIFD
                     KGFLPSTFKKPFVITDNNSEFICDKEFDDIRDGKWVISIDGEVTIRDITRLPGGRIFV
                     EGGNRAFECKIEDIEIIGKIISLTVKYVR"
     gene            1138..1329
                     /locus_tag="JQH65_004096"
     CDS             1138..1329
                     /locus_tag="JQH65_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141299.1"
                     /translation="MLGKVFFVVLSCSLLLSPLTTYAKNYPCSGKKGGVSHCTSDGKF
                     VCNDGTISKSKKICTKNSR"
BASE COUNT          397 a          241 c          299 g          396 t
ORIGIN      
        1 agccagattt ctggtgcggt taataaatga ggctgagctg ggttcagctt gttccgcaga
       61 atctgcacat tcatgcctgc acgttctgcc agttgcacca gattgtggcg cagtgcaaat
      121 gcacgacagg cttcatcaaa atgtggatgt ttggaaactt ggtaatcaaa catggtcaat
      181 gcctctgatg tatttcagaa tcgaactaat taaggtttag attgcattct gaaagcgcat
      241 caacggtcat tgctgctatg ttgatcatca ctttttcgcg ttttttatct ttgcgcagac
      301 ggtgacggat aaggcgtcca tcagccaaca tgtcattgat ggtatcgatg gatagccctg
      361 tcagctcgct atagcgttca atagtcacat gaggcgtggt aagagtgatt gaaatgttag
      421 gtctcatgat gcaacattcc tcgtttaatg atgattaatc aggacgaata cggatcgttt
      481 gtattttgtg aacaccataa acatacgatt gcacagtgaa atcgtcaaga taaaagttca
      541 cttggagtga ccatgaattt ggagaaaggc ggacgaggcg ccatagagcg catggtagaa
      601 gcttatggat tcaagactcg acaggcgttg tgcgatcatt taggaatctc taaaagtaca
      661 ctcgccacac gctacatgcg tgactcattc ccagcagaat gggtaatcca gtgcgccctt
      721 gaaacgggca cctcgcttaa ttggctcaca accggacatg gttcaaagca aacttcaggt
      781 aatacaaata ctatggaagt tgctaaatat gtattatctg atggtgcctt gcgtgaagac
      841 ggtttttata tttttgataa gggatttcta ccctctacgt ttaaaaaacc ttttgtcatc
      901 acagataaca attctgaatt tatttgtgat aaagaatttg atgacatacg tgatggtaaa
      961 tgggtaataa gtattgatgg cgaagtaacg atccgtgaca ttactcgttt acccggtgga
     1021 agaatcttcg tcgagggtgg aaacagagcc ttcgaatgta agatagaaga cattgaaata
     1081 attggtaaaa ttataagttt aacagttaaa tatgttagat aataccggga ggaaactatg
     1141 cttggtaaag tattttttgt ggttttatca tgctctttgt tattaagccc actaactacc
     1201 tatgctaaaa attatccttg ttctgggaaa aagggaggtg tctctcactg tacctccgat
     1261 ggaaaatttg tttgcaatga tggaactatt agtaaatcca aaaaaatctg tactaaaaac
     1321 tcacgataac ttt
//
LOCUS       DADLFA010000331         1327 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.333, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000331 DADLFA010000000
VERSION     DADLFA010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.333"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(170..499)
                     /locus_tag="JQH65_004097"
     CDS             complement(170..499)
                     /locus_tag="JQH65_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HAY6141300.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(671..>1327)
                     /locus_tag="JQH65_004098"
     CDS             complement(671..>1327)
                     /locus_tag="JQH65_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAY6141301.1"
                     /translation="TGEIDTALWRSGDVLLGSLLAMLFTGIWPQRAFIHWRIQLAKSL
                     TEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIAPASKETRIPKSIYEGIQT
                     INRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQQILLSLVHALYEGNPQPV
                     FANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNMETAHQLELLSNLICRALRK
                     "
BASE COUNT          326 a          310 c          311 g          380 t
ORIGIN      
        1 agtgcggtta ataacaatgg agttacagaa ctttttgcct tgctgcatac agaagatgtc
       61 tgtcgctaat ttcggtaagt tatcagatgg gaatgatgac ggtgatacac agaaaagaat
      121 caatgagcaa tgagggttgc caggcaaccc tcattgaata aaacaggaat tacttcgctt
      181 cgccgttttt catttcgccc atagctttca gctttttgga gatatcgcgg cgttctttgg
      241 agagctcggc atttttgatg atgtaatcgt caacgcgatc ttcatagtca cctttcatgc
      301 tggcgatgat gccctggatt gcttcaacgc tcatccccgg cttgatgtaa tcgctcaggt
      361 tgtccagcag caggacgcgc ttctggttgt cacggatctt tttctcaacg tcctgaattt
      421 cacgttgcag tttgttctta cgacggaaca gacgaacaaa ttccagtacg tcctggaatg
      481 aaggcttggt agtttccatt tttatacccc tgataatgtg agagtcggat tcgtttaagc
      541 aaccgctatc cgttagagcc aacattactc atagctgccg cagatgacaa tgcttttatc
      601 ccttttcact atcataccct ttatccttgc tgaatcgaag cagcagcaag atgattctgt
      661 agttcaggaa ttatttgcgc aaggcccggc aaatcagatt cgatagcaac tcaagctgat
      721 gtgccgtttc catgttcagc cacacataac catagattgg tgtttccaca accttcaggt
      781 catggtggtt attgagcaac tgacgcagct cttccactgc atcgttcaat ttttccgtat
      841 tggcaaaaac cggctgcgga ttaccttcgt acaacgcatg aacaaggctc agcagtattt
      901 gctgcatcat gtgctgggta tcacgaagtt tttgcgcgtt caataacacg aaatggctgg
      961 ggcgcgtggc ccagtacgca ttgatttgca actccagcat acaaaccaga ttgcggttaa
     1021 tggtctggat accttcatat atcgattttg gaatacgggt ttctttgctg gcgggcgcaa
     1081 tcagcccacg cattttcacg gcatcggtca ggagtttttg tagatggctt tccagacgtg
     1141 ggcgttcgag taagttcggt gagaatgcag attgatagac ccgattatac tcggtcagac
     1201 ttttcgccag ttgaatgcgc cagtggatga acgcccgttg tggccagata ccggtaaaca
     1261 acattgccag taaagagccg aggagcacat cgccgcttcg ccataacgcc gtatcaattt
     1321 cacctgt
//
LOCUS       DADLFA010000332         1311 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.334, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000332 DADLFA010000000
VERSION     DADLFA010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.334"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..740
                     /locus_tag="JQH65_004099"
     CDS             <1..740
                     /locus_tag="JQH65_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HAY6141302.1"
                     /translation="PARKIHLYHCDHRGLPLALISTEGATEWCAEYDEWGNLLNEENP
                     HQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNFYQYPLNPIS
                     EIDPQGLNPLILGVVALMSCGLFSSGGVIQSGQQRRDQYGRKVNPEADKLTDEHKSGV
                     NPGGNCSPQNLSDLQNEKNRLCNQSRACAPQMLRKEILRRYEINLACASVRKQINNQC
                     FGGGDKAHMEEENKAYKTAADCSGLIK"
     gene            737..1240
                     /locus_tag="JQH65_004100"
     CDS             737..1240
                     /locus_tag="JQH65_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141303.1"
                     /translation="MRKIIFSNESAVYDELMIHFPCQPLPHISNDIIGLDELDVVYNF
                     FQKKQWNEIANNLKIKDDSYALELGITFLPEKVFCYYIPLYIYVSLFNKNDFWVFESD
                     FIQQYLCPEYRDYDDFLNFVFNFSDIQLSIIAQFMSYESDAGFFYASKACMDFWEDYS
                     PLLHKKI"
BASE COUNT          411 a          227 c          293 g          380 t
ORIGIN      
        1 cgccggcgcg aaaaatccac ctgtaccact gcgaccatcg cggcctgccg ctggcgctta
       61 tcagcacgga aggggcaaca gaatggtgcg cagaatacga tgagtggggc aacctgctga
      121 atgaagagaa cccgcaccag ctgcagcagc ttatccgttt gccggggcag cagtatgatg
      181 aggagtccgg cctgtattac aaccgccacc gctattatga cccgctgcag gggcgatata
      241 tcactcagga cccaattggg ctgaaggggg gatggaattt ttatcagtat ccgttgaatc
      301 cgatctcaga gatagaccca caagggttaa atccgttaat tcttggtgtt gtagcactga
      361 tgagttgcgg tttgttttca tcaggtggtg ttatccaaag tgggcaacaa cgcagagatc
      421 aatatggacg taaagttaat ccagaagccg acaaattaac agatgaacat aagtcaggtg
      481 taaatcctgg aggtaactgt agtccccaga acctgagtga cttacaaaat gaaaaaaaca
      541 gattatgtaa ccagtctaga gcttgcgctc ctcagatgct gagaaaagaa atattgagac
      601 ggtacgagat aaatttggcg tgcgcttcgg tcagaaagca gattaataat cagtgctttg
      661 gtggtggaga taaggcccat atggaagaag agaataaagc atataaaaca gcagctgatt
      721 gcagtggctt aataaaatga gaaaaataat tttctctaac gagagtgcag tctatgatga
      781 acttatgatt cattttccat gccagccatt acctcatatt tcaaatgata ttatagggct
      841 ggatgaactg gatgttgtat ataatttttt ccaaaagaaa caatggaatg aaatagccaa
      901 taatttaaaa ataaaggatg actcttatgc tttagagtta ggaattacat tcctccctga
      961 gaaagtattc tgctattaca tccctctgta tatttatgtc tcgttgttta ataagaatga
     1021 tttttgggtt tttgaatccg attttattca acaatattta tgtcctgagt atcgggatta
     1081 cgatgatttt ttgaattttg tttttaattt ttcagatata caactatcta ttattgcaca
     1141 gtttatgtct tatgaatcag acgctggatt cttttatgca agtaaagctt gtatggattt
     1201 ttgggaagat tattcacctc tattacataa gaaaatttaa tcgtaaaagg tataaatggt
     1261 ttaataaaaa acttgtctac tcgttggagt agatgaaaaa taagatgacg t
//
LOCUS       DADLFA010000333         1302 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.335, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000333 DADLFA010000000
VERSION     DADLFA010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.335"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..908
                     /gene="pocR"
                     /locus_tag="JQH65_004101"
     CDS             <1..908
                     /gene="pocR"
                     /locus_tag="JQH65_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="regulatory protein PocR"
                     /protein_id="HAY6141304.1"
                     /translation="AATLNSELINKIAQDFAQATSLAVVVVNIHGDEISELFNFTPFC
                     QMMRQHPQLSTRCRMSDRCGGLEASKSDQPCIYRCHAGLTDFSIPLVIAGHLVGFVLC
                     GQVRLRNDYVDLVDILNVDDCWQADPELLNEFRKVPEMDYSRVMASADLLKLIVENCL
                     KKQLNFVVIKENKSQSDSVRQTRAPSPHDNKMKKALRYIDAHLSDDLRLEDVASHVYL
                     SPYYFSKLFKKYHGIGFNAWVNQQRMASARELLCHSDWSIASIARNLGFSQTSYFCKV
                     FRQTYQVTPQVYRQQVSENAAMETV"
     regulatory      997..1172
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT          353 a          277 c          324 g          348 t
ORIGIN      
        1 ctgcggcgac acttaattca gaactcatta ataaaatcgc gcaggatttt gcgcaggcga
       61 ccagccttgc ggttgtcgtg gtcaatattc acggcgatga aatttccgag ctgtttaact
      121 tcactccatt ttgtcagatg atgcggcaac accctcagct aagcactcga tgtcgcatga
      181 gcgatcgttg tgggggcctg gaagcctcta aatccgatca gccttgtatt tatcgctgtc
      241 atgccggatt aaccgatttc tctattccac tggtaatcgc cgggcattta gtcggttttg
      301 tattgtgcgg gcaagttcgt ctgcgtaatg attatgtcga tttggtcgat attctgaatg
      361 tcgatgattg ctggcaggca gatcccgaat tacttaatga atttcgcaaa gttcctgaga
      421 tggattactc aagagttatg gcctctgctg atttgttgaa gttgattgta gaaaattgcc
      481 tcaaaaaaca actcaatttt gtggttatta aagagaacaa atctcagtca gattcggtgc
      541 gccagactcg tgcgcctagt ccccacgaca ataaaatgaa aaaagcttta cgctatattg
      601 atgcgcattt atcagacgat ttgcgtctgg aagatgtcgc ctcgcacgtt tatctaagcc
      661 cttattattt cagtaagtta ttcaagaaat accatggaat cgggtttaat gcgtgggtaa
      721 accaacaacg gatggccagc gcgcgtgagt tactgtgcca tagtgactgg agcatcgcca
      781 gcattgcgcg taatttaggt ttttcgcaaa ccagttattt ttgcaaagtt ttccgtcaga
      841 cttatcaggt tacgccgcag gtgtatcgac agcaagtgag tgaaaatgcg gcgatggaaa
      901 cggtatagag taaatgctaa aaaaagggaa ggtttaaata aacgctggaa tgaataatta
      961 acgttattta cgtgttatgg gaaaagtaaa atcagctcat aacatacgct gacaggtgtg
     1021 gcacatttgt gcctgggaag agagtgaaaa tccctcgcag cccccgctgc tgtaatgctg
     1081 acaaacccga aaatgccact gatcgcgaga ttgggaaggt cgggagagga tgacgctaag
     1141 ccagaagacc agccagtcag tcaataccaa caacttcggt gggaagtggg ttgcccggaa
     1201 gcgtccggta gagtacaggc gcgtctttat acccctacca ccctgaaagg atcagggtat
     1261 ggcggcatca tttagttctt tcatcgcagt gccgacaggc ac
//
LOCUS       DADLFA010000334         1283 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.336, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000334 DADLFA010000000
VERSION     DADLFA010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.336"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(200..466)
                     /locus_tag="JQH65_004102"
     CDS             complement(200..466)
                     /locus_tag="JQH65_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141305.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPATNRTKTLKFTATEMQWLTKINEVGIVEKQSQRHSNI"
     gene            complement(648..1046)
                     /locus_tag="JQH65_004103"
                     /pseudo
     CDS             complement(648..1046)
                     /locus_tag="JQH65_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612066.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          325 a          266 c          271 g          421 t
ORIGIN      
        1 tgcgtccctg cactcgaata atggtgaaga aagcatgatg aatatatcac gataacgttc
       61 agtatgtatc gaggatagat ggagttactc ctctctgaaa tagaattact gctctacttt
      121 ttctgtttcc agaaattatt agtaacaaac cctgatatat tcttttgaaa atggagctta
      181 aacgagtctg aaattaactt cagatattag aatgccgttg gctttgtttt tcaacaattc
      241 ctacttcatt tatctttgtc aaccattgca tttctgtagc agtgaatttc aacgttttcg
      301 tccggttggt agcaggtatt tgtaattcgt atatagctgc ttttttattg aaaacagtat
      361 atccatctgt agtaacagta gaagataata atacaggatt tgacacttca aggatgatat
      421 tttcttgttt ttgtggaagg caagggaata tagtttgtgt aagcatattg cttccttatg
      481 ttatatttta gtaaagaatt tattttttac attatagtcg gctaaatgaa gtttactgta
      541 aaaaaacgat acttttttcg taagcgatga gatgtggagc cagaaaaatg gctgcgttac
      601 gtcattgaac tgtccttgac ccacgatatt cagacagtcg ttgtatctta agttgacatt
      661 acccgctgac gtcgatattc ccttggtgag agataaccca acgcactatg cggatggttt
      721 tcattgtaat gctcgatcgc cactgcaagg ttatgcaatg ccgttcttgg tttcggcatg
      781 aacgcgatgc agtcttcctt catcgttttc acgaaccgtt ctgctattcc attactctga
      841 gggctactga ctgccgtcgt gcagggctcc agattcaact ctctggcgaa ctgccgtgtt
      901 tcatgagccg tataagccga accgttatcg gccactggat tggccgttcc ggtcctttat
      961 cgccatatcg ctgacatgac atcctgcacc gttgctttgt cataatctcc cgtgcctgct
     1021 gcccagtcta tggcttcacg atcgcaatat ggaacgtaac gctgtcatcg ggagaatcgt
     1081 caatgcggca ttcggttaac gctgatatga ctttccgtgt caaaccggaa tctctaattt
     1141 tgacgatttt ctttttaggt ttaattccct gagggcgacg tagcagtaaa tccgacggtg
     1201 aatcacgctg atattcgcgg gttggatacc gcattacaga ggtccgtcat atgagcctgt
     1261 cactttctta cgtcgcgaat cag
//
LOCUS       DADLFA010000335         1261 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.337, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000335 DADLFA010000000
VERSION     DADLFA010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.337"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..1174)
                     /locus_tag="JQH65_004104"
     CDS             complement(<1..1174)
                     /locus_tag="JQH65_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY6141306.1"
                     /translation="MVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINR
                     FGQKRAFIIGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGS
                     TSKKTGTSVNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMIL
                     RYFFIGESAMWAARVGRYQEACDILGKRYGVQARVAASGTTEAKFSEKAENKYSGGYG
                     ILFNDRYRKRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALC
                     GVTGGMIGSFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHS
                     AGPGGLGMTIATLSYLPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWL"
BASE COUNT          372 a          355 c          280 g          254 t
ORIGIN      
        1 ccaaccagta aaacgcttca gttttcaacg caccccacag catcggccag aaaatgagtc
       61 ctgcaattgc ccctgtgcgc ataatagcgc gggcaaatcc gaccccagta gggcgaatag
      121 cgggaagata agagagtgtg gcaatggtca tgcccagccc accaggaccc gctgaatgga
      181 agaaaataat tgatcccaat aaccctaaag acagccaggg attagtcgtt gccagtgcgc
      241 ctaacgacag caatgctaag gtcacaacag caaatccata catcgactgt cggcgagtac
      301 ccagtcgttg gagaataaac gagccgatca tcccgcctgt caccccacaa agggcattca
      361 cgacggcaga accggtcaac gcgccagtaa gcccgccact tattattccc gccaacgtaa
      421 gaggaagata aacacctacg gcgttatatt gccacgcctg catggttgcc acgacacagc
      481 caagaatggt gcgtttgcgg taacgatcat taaataagat gccatatcca ccactgtatt
      541 tattctctgc tttttccgag aatttcgctt ctgttgtacc cgatgccgca acgcgagcct
      601 gaacaccata acgttttccc agaatgtcgc acgcttcctg gtaacgcccg acgcgtgcgg
      661 cccacattgc ggattcacca ataaagaagt aacgcaaaat catgaagatg actgcaaaaa
      721 tagctccgat gaatattccg taacgccaca attgttcttc agcgataccc gacagaagca
      781 gcggcaaaag taccaaataa acaacggttg tcgaaacata ccaggccatt tgccagaggt
      841 tgaccgacgt tccggttttc ttcgacgttg aaccacgaag ctctgccacg gcattggttg
      901 ccaaagggaa gtcgatgcct aaaccaaagc ccatgatgac gcggcacacc agcacccacc
      961 agatactggg agcaatagct accgctgcag caccgatggt gcacagcccc atgccaataa
     1021 taaatgcccg cttttgccca aagcggttta ttactcggtt cgctaataaa ccgccaatta
     1081 acgcggcgat taatactgat gcattgacgg ttgcagcaag acccggcgaa atgccgaatt
     1141 gagcggcaat gtatttatta ccaaacccca ccatcgccgc ctagtaggca tcaatcaata
     1201 tgccgcccaa agcaataaaa ataacgccga tactggtgtt gattgcagag ttcttattaa
     1261 c
//
LOCUS       DADLFA010000336         1213 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.338, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000336 DADLFA010000000
VERSION     DADLFA010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.338"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            540..1196
                     /gene="pphA"
                     /locus_tag="JQH65_004105"
     CDS             540..1196
                     /gene="pphA"
                     /locus_tag="JQH65_004105"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HAY6141307.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVCAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
BASE COUNT          335 a          229 c          297 g          352 t
ORIGIN      
        1 ctgcttaaaa aaagaacaaa tggtagtgtc cgctattgcc agtacacctc agtcgcaacc
       61 ccaaatcgta aaggaaaccg tagcacgtcg tatgcaagaa cgtgccacgg ctggctgatg
      121 gatgttcgat agcgtgagtt tgaatgaaaa tcagccggag atgattttac ataattgcta
      181 cggaattatt caatacagga attgcttgcg tctgcatgga ttgacctgaa attttcccga
      241 aaatttctct aaaaaactcg aaaaaaatgg taactggttg aatgtattaa tatgcaatgg
      301 tacgtgtcag ggattaaaag atgaacgtaa atttattcaa cgcattaatt ttaaagggtt
      361 ttattgtttg ttgacgaaaa caggaatcgt gttcggtctc tttttatctg ttaaaagcca
      421 gaagcatttc cttcgctgac tttatagtca accataacac acactctact gtctgagtcc
      481 agcgtttttt aacattcttg ttaagattat gtgatcttta gcacgggagg aaaatattga
      541 tgaaacagcc tgcgcccgtt tatcagagaa ttgcgggtca tcaatggcga catatctggc
      601 tttctggcga tatacacggt tgtcttgagc agttgcgccg caaattatgg cattgtcgtt
      661 ttgatccgtg gcgagattta cttatctcag tgggagacgt tatcgatcgt gggccgcaaa
      721 gtttacgttg tctgcagtta ctggaacaac attgggtttg tgcggtaaga ggcaatcatg
      781 aacagatggc gatggatgcg ctggcatccc agcagatgtc tttgtggttg atgaatggcg
      841 gcgactggtt tattgcgctg gcagataatc aacagaaaca agcgaaaacg gcgctggaaa
      901 aatgtcagca tttgcccttt attcttgaag tacacagtcg caccggcaag catgttattg
      961 ctcatgcgga ttatccagat gatgtttatg aatggcaaaa ggacgttgat ttgcatcagg
     1021 tcttgtggag ccgctcgcga ttaggtgaac gccaaaaagg gcagggaatt acaggtgctg
     1081 atcatttctg gtttggtcat acaccgttgc gacatcgcgt ggatattggc aacctgcatt
     1141 atattgatac cggtgctgtc tttgggggcg aactgactct tgtgcaattg caatgattaa
     1201 aaatcaccgt act
//
LOCUS       DADLFA010000337         1210 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.339, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000337 DADLFA010000000
VERSION     DADLFA010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.339"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
BASE COUNT          370 a          209 c          237 g          394 t
ORIGIN      
        1 ttcaaaataa acgtttaata gggacaatca ccatttggat tgtccgctga ggaggcgaac
       61 gttttcctag acagctttat catgtcagcc cacattgttg gcgctgacaa tactaaggac
      121 aaacgatggg gaagtagctt tttagcgtta ttttcgggag taaaaaacat ggtatctttc
      181 cgtatgtcag ttgtatcttg ctggtattcg tagtcgtgat aaggcataca acgatattta
      241 tcttcacgtt agtgaagaat aagttgtttt gtgatctatg ttaatctggg attacaatcc
      301 gggcttgata catcgcatca cgacttttat tctcttcaga atagaatatg ttactccccg
      361 ttttacaggc tcaggatttg aattacaaat tatactttta agtgaacaat aactaggggt
      421 atttcttatt tcctgaaacc aggcttggcc catttcggga tgagtttgaa aaaactcttg
      481 acgcatacga tagtgatgaa ataaacgaaa caaattacgt caacaaactc aggcgtcttg
      541 cctgacatgc tcctgacatc attgatattc acgctcatct ggcatatgca tttgcgctct
      601 ttatgctgca attctggcga atgttcattt acagcgtcat caggatgccg ttatactcat
      661 tgataaaata ctaacatata accctgagaa taatcagggg acacgctggt tactggggac
      721 ggaattgtta cgtgctggca agcatgacag ggctctgtac gtattgacag gacatgtcaa
      781 tgaatttccc ccctactggt acgagcttgc tcttttacat tttatgaatg aggcatatat
      841 taatgctgca acggcatttc gatatggatt tttaacaagc atatattgtt gaaatgcttt
      901 gttagaatct ttatccattc cctggggctg tatagtatga tttttcaggt atttctgaat
      961 acagccaaat attattgcaa aatattctct aaattattag ttaaatatcc ataagcatta
     1021 ttgcctggga actggacata taattactct tctgtgctaa aggaacgttc tgaaatgttt
     1081 aagtatgcag agatgttgat ggcggaagaa gataataaag tatgtgaact tatttttaaa
     1141 caacaagata atttgatttt aaaaataaat gatagatttc ccagaataat tataaagaaa
     1201 taccgaacat
//
LOCUS       DADLFA010000338         1207 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.340, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000338 DADLFA010000000
VERSION     DADLFA010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.340"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(48..1190)
                     /locus_tag="JQH65_004106"
     CDS             complement(48..1190)
                     /locus_tag="JQH65_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001488780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HAY6141308.1"
                     /translation="MSGWFDLTLNQRVYNQDGKTANAVVTYDGNVGEQYNDAWFGDSA
                     NENIMQFSDIYLTTRGFLPFAPEADFWVGKHKLPQYEIQMLDWKTLTTDVAAGVGIEN
                     WALGVGLFDMSLSRDDVDVYSRDFSRTSQMNTNSVDVRYRNIPLWDDATLSLMAKYSA
                     PNKTDQQQDNENDNSYFEMKDSWMLTSVLRQNLQRDTFNEFTLQVANNSYASSFASFS
                     DASNTMAHGRYYYGDHTNGIAWRLISQGEMYLTDNIIMANALVYSHGEDVYSYESGAH
                     SDFDSIRTVIRPAWIWNTWNQTGLELGWFKQQNKTQQGVTLNESAYKTTLWHALKVGE
                     SILGSRPEIRFYGTYINILDNELSNFKFNENSKDEFMAGIQAEVWW"
BASE COUNT          345 a          294 c          221 g          347 t
ORIGIN      
        1 aaaccttttt taaatcctga catacacaac tccttaacta aaatagatta ccaccagact
       61 tccgcctgga tgccggccat aaactcgtct ttgctgttct cattaaactt aaaattcgat
      121 aattcgttat ccagaatatt gatatacgtt ccatagaagc gaatttctgg tcgtgaacct
      181 aaaatgcttt cacccacttt caatgcatgc cagagtgtcg ttttataagc cgattcattt
      241 agtgttacac cctgctgagt tttgttctgt tgcttaaacc agcctaattc aagccccgtc
      301 tgattccatg tattccagat ccaggccggt cttattacag tgcgaatact gtcaaaatca
      361 ctatgagcac cactttcata actataaaca tcttcgccgt gagaatagac aagcgcgtta
      421 gccataataa tattgtcagt aagatacatc tcgccctgag agattaaacg ccaggcgatc
      481 ccattggtat gatcaccata gtaatagcga ccatgcgcca tcgtattact ggcatctgag
      541 aaactggcaa aactgctggc ataggaatta ttggcaacct gtaacgtaaa ttcattaaac
      601 gtatcgcgtt gcaggttttg ccgtaaaaca gaagtcagca tccagctatc tttcatttca
      661 aaataactgt tgtcattttc gttatcttgt tgttgatccg ttttattagg tgcggaatat
      721 ttagccatta atgataatgt cgcatcatcc cataacggga tattgcgata acgaacatcc
      781 acagaattgg tattcatctg gctggtacgc gaaaaatcac gagagtaaac atcgacatca
      841 tcacggctta aggacatatc aaacagccct acaccaagtg cccagttttc aatccccaca
      901 cccgcggcga catccgtggt taaggttttc cagtccagca tctggatctc atattgcggg
      961 agtttatgtt tgccgaccca gaagtctgcc tctggtgcga agggtaaaaa acctcgcgtt
     1021 gtcaggtaaa tatcgctgaa ctgcatgata ttttcattgg cactgtcacc aaaccaggca
     1081 tcgttatact gctcacccac gttgccatca taggtaacga ccgcatttgc cgttttaccg
     1141 tcctgattat aaacacgctg atttaaggtc aggtcaaacc aaccgctcat ctcattacca
     1201 aaacgtc
//
LOCUS       DADLFA010000339         1199 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.341, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000339 DADLFA010000000
VERSION     DADLFA010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.341"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            60..1070
                     /locus_tag="JQH65_004107"
     CDS             60..1070
                     /locus_tag="JQH65_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAY6141309.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVASFRAPGFARSQHIVPARNCHVAPASLPLKVLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
BASE COUNT          283 a          294 c          325 g          297 t
ORIGIN      
        1 gtcgatgaag gtatcgcgca acgctaatcc cctatggata tcgattctgg agaataacaa
       61 tggataaaat tacccaggtt ttattttcag atattgggaa agtcaccact caatatgttg
      121 aagtaccaca ccaggaactt aaaccgcacg aagtgcggat tgcgcctgtg ttctatggga
      181 tatgcggttc ggatctgcat gttctgaaag gcggtcatcc gtttgccaaa ccacctgtcg
      241 tccccggtca tgaaattgca gcgcgcgtta cggaagttgg cagcgacgtt aaaaatgtac
      301 agccgggcga tcatgttgtg gtcgatccca tcatggcttg catggaatgc cgagcctgca
      361 aagcaggacg ttttaatctt tgtgaaccac cgcaggttgc tagttttcgc gcaccgggct
      421 ttgctcgctc acaacacatt gttcctgcgc gtaattgcca tgtcgcacca gcctctttac
      481 cgctaaaagt gttggccttt gccgaaccgg cggcttgtgc ccgtcactgc gttaaccgaa
      541 tgccgaaagc ttctctggaa agcgtactgg taattggtgc cggaacgata ggcttatcca
      601 tcgtgcaggc actgcgcatt atgggggcag gtaagattac cgtgattgaa cctgacgctg
      661 ccaaacgcgc gctggcgtta aagctgggcg cagcagaagt ttgggcacca ggtgagctgg
      721 ccgcagatgt gcgatttacg ggggcgattg atgtcgttgc agcgcaggcc acgcttaacg
      781 atgcatgtac ccgtgtatat gccggaggca ccgtcgtgtg catgggcgta ccaagtgggc
      841 cgcgtgaaat accattaccg atgatgcaac gtttcgagcg tgacttgctc aactctggca
      901 tgtacatccc tgaagatttc gatgctgtta tcgaatggct ggcggatggg cggtttgata
      961 ccagtgaact ggttaccgat ttatttgcca ttgaggatgc agcggcggca tttgaacgcg
     1021 cgcagcaaaa tgactccata aaggtcatgc tgcaatttgc gccggaatga ttttctaaat
     1081 ctctgtctgg cgctgcgtca cgtcagcgcc accctacaac aattaaaaag gacactgcga
     1141 tgagtcaaat caccagtcct gcaacttatt caattagtcg gccacaggac gtaattgat
//
LOCUS       DADLFA010000340         1199 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.342, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000340 DADLFA010000000
VERSION     DADLFA010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.342"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..755)
                     /gene="ipaH4.5"
                     /locus_tag="JQH65_004108"
     CDS             complement(<1..755)
                     /gene="ipaH4.5"
                     /locus_tag="JQH65_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HAY6141310.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQRLKYLKVGENQLRRL
                     SRLPQELLTLDVSNNLLTSLPENIITLPICTNV"
BASE COUNT          342 a          214 c          258 g          385 t
ORIGIN      
        1 ttaacattcg tacaaatggg caatgtgatt atattttcgg gtaatgaagt tagtaggtta
       61 ttggaaacat ccagtgtcaa tagttcttgc ggtaatctgg ataatctgcg tagttgattt
      121 tcacctacct tcaaatattt taagcgctga ggaaattctg gtagttcctg tagtctgttg
      181 ccggaaacaa agagttcttg tagtctatga ggcaattcag gcaagacagt aaggcgatta
      241 ccttcaatac gtaatattgt tagcgttgat ggaagctctg gtaacgtctc cagactatta
      301 aaacgtgccg agagtgattg taaatatggt ggtaatgatg gcagacatga caaatttcga
      361 ttgaatgaaa tattaagctt aatcagttga gatggaagtg tcgggagtat attcaagttg
      421 ttaccattta catgaagttc tgtcaaaagc ggagaatttt caggtaggga gattaactca
      481 ttgttggaaa tattaagttc acgtatatgc aaagggagtg gtggtaaaga acgtagtttc
      541 agtaagctta aattgagaac aggctcccgg gtatccagac atattttcaa taatcgaacc
      601 gcctggatgc gattttctgt tgttgttcct tccctagccc agtcatccca gatgcggtgg
      661 taatctctgg tacactgttc ttctaccgat aaacgagata tacatgataa gccacaaagg
      721 gaacgaaaaa aagaatgatt gttgatcggt ttcatttatt aacccctcac tattcatatc
      781 ttggtctatt aatgcaaata aaacgagaaa gtctttaaaa aaacaatcct agagctttat
      841 cggcgttttc attccagctg gaggaatatg ttcctgtctt tccagtaacc gtcgattgtt
      901 atatcagtct acccatgtta atgtgaccag ttctactttc atccagttta gattgatctt
      961 tatcccctat attttcaaac aatttttatc atttaactca ttactgtctc gatattccca
     1021 tagcaaacga tctggttggt tagcccctca aaaaagtcag ttgcacgccg taaatccctg
     1081 ccggtattga gcgaggattt acgggttgtt gttaaggtcc gtaatcgggt cacagttttt
     1141 ccggagtcaa tccggtctgc ggtttatgct tatgcgacgt gattatgaat ggtgcagtc
//
LOCUS       DADLFA010000341         1194 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.343, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000341 DADLFA010000000
VERSION     DADLFA010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.343"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..78)
                     /locus_tag="JQH65_004109"
     CDS             complement(<1..78)
                     /locus_tag="JQH65_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141311.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSN"
     gene            267..797
                     /gene="cybB"
                     /locus_tag="JQH65_004110"
     CDS             267..797
                     /gene="cybB"
                     /locus_tag="JQH65_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HAY6141312.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            1042..>1194
                     /locus_tag="JQH65_004111"
     CDS             1042..>1194
                     /locus_tag="JQH65_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141313.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISAS"
BASE COUNT          319 a          258 c          271 g          346 t
ORIGIN      
        1 gttgcttttg acttcaagta atcgacgcaa caccagtcga ccgatacgac caaaaccgtt
       61 aataccaacc ttactcatgg ttttctcctg tcaggaacgt tcggatgaaa attgatcctt
      121 tccaggctta gcccaggatt gtgggatggg caatctccat tcttacagtg aaacgtaacg
      181 tactgaaaac gggtgaacaa tatttaatga aattttgaga aaagcccgtt atgttaacgg
      241 aaaattatgt taaagcagga aatgttatgg aaaataaata ctcaaggtta caaatcagca
      301 ttcactggct ggtctttttg ctggttatcg cagcgtattg cgcaatggag tttcggggtt
      361 tcttcccacg tagcgatcgg ccactcatca acatgattca tgtttcctgc ggcatctcaa
      421 ttctcgtgct gatggtcgtt cgtcttttgt taaggctgaa atacccaacc ccgccgatta
      481 tacctaagcc aaaaccgatg atgacgggac tggcgcattt gggacatttg gtgatttatc
      541 tcctgtttat tgcgctgcca gtgattggtt tggtgatgat gtataaccgg ggcaacccgt
      601 ggtttgcgtt tggtttgacg atgccttacg cttcagaggc caatttcgaa cgggttgata
      661 gcttaaagtc gtggcatgaa acgctggcga atctgggata ttttgtcatc gggttgcacg
      721 ctgcggctgc actggcacac cactatttct ggaaggacaa cacacttcta cgcatgatgc
      781 cgcgtaaacg ttcctgaagg atatttaaag aaaacgcctg tactaaaacc gacccgtggt
      841 acaggcgaag aatacgggtc tacatcggaa gcgcctatta tatttatttg tatgacaaat
      901 aaaaccataa tccttgccca tacgtccatc tggcttattt ttaatcaatt cacccgatct
      961 ttgatctcat caacggtatc aaaataaacc agcgtataac gttcaaatcg aacataagat
     1021 tcgataaacc atggaggtta tatgaaaaaa ctggcactta ttttgtttat gggaacgctt
     1081 gtttcctttt atgccgatgc cgggcgcaaa ccctgttctg gttcgaaagg ggggatctca
     1141 cactgtacgg caggcggcaa atttgtctgt aatgatggtt ctattagtgc ctcg
//
LOCUS       DADLFA010000342         1160 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.344, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000342 DADLFA010000000
VERSION     DADLFA010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.344"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..925
                     /locus_tag="JQH65_004112"
     CDS             <1..925
                     /locus_tag="JQH65_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HAY6141314.1"
                     /translation="RRQDFLTFFGNREPCLIGMEACGGSQHWARELTKLGHKVRLLQA
                     RFVKAFVMGNKNDVMDARAIWMAVQQPGKEIAVKTEEQQSVLVLHRTRMQLVKFRTAQ
                     INALHGTLLEFGETIHKGRAAMEREFPEALERMKERLPPYLIMVLENQYNRLNELDSL
                     IEDIEKQLTSVARQNETCKRLLDIPGVGPLIATAAVATMGEASAFKSGREFAAYVGLV
                     PKQTGSGGKVRLLGISKRGDTYLRTLFIHGARAVALVAKEPGPWITELKKRRPASVAI
                     VAMANKLARTVWAITAHDRKYDRNHVSIRPY"
BASE COUNT          324 a          262 c          333 g          241 t
ORIGIN      
        1 gcgtagacag gattttctga cgttcttcgg caaccgtgag ccatgcctga ttggtatgga
       61 ggcctgtgga ggttctcagc actgggcacg ggaactgaca aaacttggtc ataaagtccg
      121 gttgttgcag gcccgcttcg ttaaggcatt cgtcatgggc aataagaatg atgtgatgga
      181 tgcccgggct atctggatgg cggttcagca gccgggtaaa gaaatcgccg taaaaacaga
      241 agaacagcag tcggtactgg ttctgcaccg tacccgcatg caactggtga agttccggac
      301 cgcacaaatt aatgccctgc acgggacgtt actggagttt ggtgaaacca tccacaaagg
      361 ccgggcagcg atggagcggg agttccccga agcactggaa cggatgaaag agagactgcc
      421 accgtatctc attatggttc tggaaaacca gtacaaccga ctgaatgagc tggactcact
      481 gatagaggat attgaaaaac agcttaccag cgtggcgagg cagaatgaaa cctgtaagcg
      541 gttgctggat attcctggcg ttggaccact tattgcgacg gcagcggtgg ccaccatggg
      601 ggaagcatca gcgtttaaat cggggcgaga gttcgccgca tatgttggtc tggttccaaa
      661 acaaactggc tccggaggga aagtacgtct gctggggata agcaaacgtg gtgacactta
      721 tctcaggaca ttatttatcc acggtgcaag agcggtggca ttagtagcta aagagcctgg
      781 cccgtggata accgaactga aaaaacgtcg tccagccagt gtggcaatcg tcgccatggc
      841 aaacaagctg gcacgaacag tatgggcgat aaccgcccat gaccgtaagt atgacaggaa
      901 ccacgtcagt atcagaccat attaatcgct gataccatta aacaatgaac tcttaacaaa
      961 agggtgaatg ctgaaaggtt gctatggcgg ccagagtgat gacaaagaca ggtaagaccg
     1021 tgactcacta aacctgaaca gtattttggg cttgaagtcc gccgtgaaaa taaggggtga
     1081 gtcggcgaat tacatagggg ctcgcagcgt tacggctgca ataaagccgg atataaagct
     1141 gcaacctacc cgtcatgtca
//
LOCUS       DADLFA010000343         1154 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.345, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000343 DADLFA010000000
VERSION     DADLFA010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.345"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(1..261)
                     /locus_tag="JQH65_004113"
                     /pseudo
     CDS             complement(1..261)
                     /locus_tag="JQH65_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(376..1014)
                     /locus_tag="JQH65_004114"
     CDS             complement(376..1014)
                     /locus_tag="JQH65_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141315.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
BASE COUNT          337 a          285 c          175 g          357 t
ORIGIN      
        1 caagcgctgt aactctttta tttgttttat agcggcagcc agttctgagg caggaacgac
       61 atcctctccg gcagcaacag cagtgagact tccttccaga tattattttt tccacttaag
      121 caacaggctg ggctgaatac catgcaaacg cgccacatgg gacaccgtca tgccaggctc
      181 cattgtctgc tgaataatgg caattttttc ctgaggagtt ttacgtttac gaagctccag
      241 ccccagcaaa gttccagtca tctcaaaatt agaactagtg ttagacatat atccaagcct
      301 atctcttatt ttaagataca tccactgtct ggcgtttcaa ggggccggct caaatctatg
      361 tacaggctct cagcatcagc aaagcctctt actcttccaa ctactactgt tagcatagtg
      421 atcaaattcc tgatcagata catgaattac ctgaaccgaa ctattttcat ctacaaatgc
      481 atctggcgat ttttctacag gatcataaag attaaatccc tttcctgttt gaacatccaa
      541 aaacataatt ttttcaccat cgttaaatcc agctatccaa tgagtggcct gttcaataga
      601 aatcattact acacttcctt ttgaagtgtt ttgtaaaata ttttctgcaa tacgctcacg
      661 aaatttacta taagtttcat taggatgtga cactatgcct tcactatcaa tttgctctaa
      721 atgcctatga aaaaatccgg tcatatactc tgatatttca ttaggtacgg gagcctctct
      781 tactccatac cctaacatag ccatacaagc atgaagcgca caccctgaac agttaccatc
      841 cccaccagac ggatttatca ttttctgtaa agtcgctgcc aattcagaat ctttatccat
      901 atcaccaaga cttttagagt ttaaagattc cagcaatttt ttaacattta catttccctc
      961 ctctgtatgt gcctcatatc cagcagttcc ctgtaacgac accccattaa tcatattgta
     1021 tcctgacagt atatatactc actatatttt cttcataaca aatatccctc tgatatgaca
     1081 acaaaaaacg gagccggatt cctgtttttg tgaactgcag ctattcgagt ctaaaaagtg
     1141 atccacttac cacc
//
LOCUS       DADLFA010000344         1130 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.346, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000344 DADLFA010000000
VERSION     DADLFA010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.346"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..752)
                     /locus_tag="JQH65_004115"
     CDS             complement(<1..752)
                     /locus_tag="JQH65_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY6141316.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGISTPKVTRAHNRAEAVFLC
                     VMHSHIQIMVRRAGQPQGWPGSRVTGSANPVRLTTHEISTSAGELINLSLEDVIMATT
                     LSHPDVTIENGRAVTTSISIAEFFGKQHHHVVQKIESFECSELFLTSNFSRVKFEHRG
                     NSYNAYQITKNGFVFLVMGFTGKKAATFKEAYIAEFDRMEAELRQNNTPPADKMIPGD
                     GRTLVVHFDKFGNVEFTETVPDGALVCTLETFR"
BASE COUNT          289 a          283 c          267 g          291 t
ORIGIN      
        1 cggaaagtct ccagggtaca gaccagtgcg ccatcaggaa cggtttcggt gaattcgaca
       61 ttgccgaatt tgtcgaagtg aacaaccaga gtgcgaccat cacccggaat catcttgtca
      121 gcaggtgggg tgttattctg gcgcagttcg gcctccatgc ggtcgaactc agcaatgtag
      181 gcttccttga atgtggcggc ttttttgccc gtgaagccca tcaccaggaa aacgaagccg
      241 tttttggtga tttggtaggc gttataagaa ttgccgcggt gttcgaattt aacccgcgaa
      301 aagttgctgg ttaaaaatag ttcagaacat tcgaaggatt ctattttttg aacaacatgg
      361 tgatgctgct tgccaaaaaa ctcagcaatc gaaatagaag tagtgacggc gcgaccattt
      421 tcgatggtta cgtcagggtg agaaagggta gtagccataa tgacatcctc aagtgataag
      481 ttaattaact caccagcaga ggtgctaatc tcatgggtgg tgagacgcac agggttagca
      541 ctaccggtca ctcgagaacc cggccagcct tgcggctgcc ccgcacgcct caccataatt
      601 tgaatgtggc tgtgcattac gcataaaaaa accgcttcag cgcggttatg cgctcgagta
      661 actttcgggg tgctaatccc ggcacccgtt ttatgaggtg caggtgcact ataattccac
      721 ccgttctggt tttcaatagc tacattcaac attttctctt tcctttcatc accgaagtga
      781 attttgtgat gcggtgccta gtgcctccag gtgacgttaa ccagttaaca attacagtcg
      841 gctttcccac ccaaaccaat aaggactaac atgactttta actgtgccgc gtgcgcttag
      901 ccgcattcac cgcatcacaa aattcacttt aaaaagggcg gacatcagcc agcaattaaa
      961 ccgatgccgc caactggtac ttcacacagc aatgtcgtta tttacaaccg gaagcgcact
     1021 cccaccattt aaatttcaca gacaagaccg actctttatg aataccggaa atgcgccttc
     1081 gtgttgtgcc cggttttatt tcaccacctc cgggctttgg tggcctcggc
//
LOCUS       DADLFA010000345         1129 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.347, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000345 DADLFA010000000
VERSION     DADLFA010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.347"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            66..1074
                     /locus_tag="JQH65_004116"
                     /pseudo
     CDS             66..1074
                     /locus_tag="JQH65_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
BASE COUNT          271 a          307 c          296 g          255 t
ORIGIN      
        1 gatccacacc caacgcctga aatcagatcc agggggtaat ctgctctcct gattcaggag
       61 agcttatggt cacttttgag acagttatgg aaattaaaat cctgcacaag cagggaatga
      121 gtagccgggc gattgccaga gaactggggc tctcccgcaa taccgttaaa cgttatttgc
      181 aggcaaaatc tgagccgcca aaatatacgc cgcgacctgc tgttgcttca ctcctggatg
      241 aataccggga ttatattcgt caacgcatcg ccgatgctca tccttacaaa atcccggcaa
      301 cggtaatcgc tcgcgagatc agagaccagg gatatcgtgg cggaatgacc attctcaggg
      361 cattcattcg ttctctctcg gttcctcagg agcaggagcc tgccgttcgg ttcgaaactg
      421 aacccggacg acagatgcag gttgactggg gcactatgcg taatggtcgc tcaccgcttc
      481 acgtgttcgt tgctgttccc ggatacagcc gaatgttgta catcgaattc actgacaata
      541 tgcgttatga cacgctggag acctgccatc gtaatgcgtt ccgcttcttt ggtggtgtgc
      601 cgcgcgaagt gttgtatgac aatatgaaaa ctgtggttct gcaacgtgac gcatatcaga
      661 ccggtcagca ccggttccat ccttcgctgt ggcagttcgg caaggagatg ggcttctctc
      721 cccgactgtg tcgccccttc agggcacaga ctaaaggtaa ggtggaacgg atggaacagt
      781 ttttacatcc cactaatgac tcgcctgcgc ccgatgggga gcactgtcga tgttgaaaca
      841 gccaaccgcc acggtctgcg ctggctgcac gatgtcgcta accaacgaaa gcatgaaaca
      901 atccaggccc gtccctgtga tcgctggctc gaagagcagc agtccatgct ggcactgcct
      961 ccggagaaaa aagagtatga cgtgcatcct ggtgaaaatc tggtgagctt cgacaacccc
     1021 ccccagcatc atccactctc catctacgac tcattctgca gaggagtggc gtgatgatgg
     1081 aactgcaaca tcaacgactg atggcgctcg ccgggcagtt gcaactgga
//
LOCUS       DADLFA010000346         1081 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.348, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000346 DADLFA010000000
VERSION     DADLFA010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.348"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..772)
                     /locus_tag="JQH65_004117"
     CDS             complement(<1..772)
                     /locus_tag="JQH65_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY6141317.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSH"
BASE COUNT          310 a          154 c          253 g          364 t
ORIGIN      
        1 catgggatga caatgggtta tcactaatat ctattgaaca ttcattcctc agattaagaa
       61 tgctttccgg gatatgactt atctgattta tgtcaaaata accgttcctt aaattttccg
      121 gcaggtatgg aatatcggtt aacatattgt cgattgccca tatggagtga atgttgtgag
      181 gtagaggggg gagttcacta agaagattcc ctattgcgct aatctcttgt aaagaaaatg
      241 gtaaaggtgg taaaactgcc agcccattac atgatacatc taatgtttcc agtagttctg
      301 gtaaaacagg aagagaacat aattgattat ttgagacatg aagctccttc aaagatatgg
      361 ggagtgtggg tagtgtgatt agttgattgt gggacgcatt caataattta agtccttgag
      421 gcaaagcagg cagttcaata agtctgttat agctgacatt aagctgtgta agggacgcag
      481 gcaatgggga gattaagctt aaattatttt tacttatatt aattgattta attcccgggg
      541 ggatttcagg taatgttgtc aggcctaatt cagacaagtc taggttcgtc tcttggtttt
      601 gtagacatga tactagtcgc tgaaaagcga tgtctcgttg ttcttcttgt atgcggttat
      661 ttttccattc agtccaatgg gttagataat tttcatatgc gcggctagtg tcgattgaat
      721 aagtggagaa cgaatttgaa attaatctgt gattgttatt tgtcgggagc ataaatatca
      781 gggcgtattc tttatgttga gaggtgttgg attctttttt atctgctttg caatgttatt
      841 ggttcccttc agcagtagac aggaacttct ggaggaggcg gatatatagt ttgtgtaagc
      901 atattaactc catatgttat atattgaaga acttctgctt tacactatcc taaactggat
      961 cggtttaatg taaaaaaacg atactttttg ggggaggggg caatttttcc ttaaggtatg
     1021 aaaaaaggag ctgaagctat ataatagcta ttaatgccac tggtatcagt gaatcatgta
     1081 t
//
LOCUS       DADLFA010000347         1056 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.349, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000347 DADLFA010000000
VERSION     DADLFA010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.349"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..1045
                     /locus_tag="JQH65_004118"
     rRNA            <1..1045
                     /locus_tag="JQH65_004118"
                     /product="16S ribosomal RNA"
BASE COUNT          261 a          247 c          330 g          218 t
ORIGIN      
        1 actccgtgcc agcagccgcg gtaatacgga gggtgcaagc gttaatcgga attactgggc
       61 gtaaagcgca cgcaggcggt ttgttaagtc agatgtgaaa tccccgggct caacctggga
      121 actgcatctg atactggcaa gcttgagtct cgtagagggg ggtagaattc caggtgtagc
      181 ggtgaaatgc gtagagatct ggaggaatac cggtggcgaa ggcggccccc tggacgaaga
      241 ctgacgctca ggtgcgaaag cgtggggagc aaacaggatt agataccctg gtagtccacg
      301 ccgtaaacga tgtcgacttg gaggttgtgc ccttgaggcg tggcttccgg agctaacgcg
      361 ttaagtcgac cgcctgggga gtacggccgc aaggttaaaa ctcaaatgaa ttgacggggg
      421 cccgcacaag cggtggagca tgtggtttaa ttcgatgcaa cgcgaagaac cttacctggt
      481 cttgacatcc acggaagttt tcagagatga gaatgtgcct tcgggaaccg tgagacaggt
      541 gctgcatggc tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc
      601 aacccttatc ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata
      661 aactggagga aggtggggac gacgtcaagt catcatggcc cttacgacca gggctacaca
      721 cgtgctacaa tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa
      781 gtgcgtcgta gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta
      841 atcgtggatc agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac
      901 accatgggag tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac
      961 tttgtgattc atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg
     1021 gatcacctcc ttaccttaaa gaagcgtact ttgcag
//
LOCUS       DADLFA010000348         1048 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.350, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000348 DADLFA010000000
VERSION     DADLFA010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.350"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(452..824)
                     /locus_tag="JQH65_004119"
                     /pseudo
     CDS             complement(452..824)
                     /locus_tag="JQH65_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          255 a          249 c          244 g          300 t
ORIGIN      
        1 ggggaaaaac gtgtattttt agtcattctg tttacctctt tctcagggcg tttagtctcc
       61 aggatttccg gggcggttca aaaaactctt cgttgattac tgtggactta ctgttggcat
      121 taccgaccct gagaccggag aaataagaac cgcttagagc atcgtggctg ttctcggggt
      181 atcaagttac acatgagcag aggctaccca aggtctacta ttatcgcctg aaagcgctga
      241 tggaacagtg ctatcaggga catgctgatg aaaataccta aataatcagc cagcactcta
      301 tctttccaaa tccacagcat agcaaagaga gcaaaagagc ctgtaaattc agaaattaag
      361 gaaatgcctc ttggtgtaca taatctatct tatgttaaat gaaatctaat ttcttgatta
      421 tatgctggat cgttcgtggg tggctttgcc cctatatttc cagacacctg ttatcattgg
      481 cccgctgccg cagatattcc cgtggcgagc gataatccag cgcactatgc ggatgccatt
      541 cgttataatg ctcgaatgcc tctgcaaggt tctttgctgc cgttaacccg tcaggtttgg
      601 gcatgatact gatgtagtca cactttattg ttttcacgaa gctctttgct attctgttac
      661 tctccggact ccgcaccgcc gtgttcttcg gttcaacaaa gtcccaacat ccgggtgaac
      721 tggcgtgttt cattagcccg gtagcatgaa ccattatccg tcagtcactt cactggagac
      781 gccggaagct cgttgccgaa gcggtgttcc accgctccca gcatgtcgcc aacgggagag
      841 catctacggt ctgattgaac tgtttattca aattgtccac cgtctgtcag tacgctctga
      901 acgtcggctg gttaaaacac tgctcgctga ttttcagagg gttcacggta aaacagcact
      961 tttattccgg attgcggaag ggctctgaat aatccggatg ggctggttaa agaggttgtg
     1021 taccatctgc atattgttga accagatc
//
LOCUS       DADLFA010000349         1044 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.351, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000349 DADLFA010000000
VERSION     DADLFA010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.351"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..510
                     /locus_tag="JQH65_004120"
     CDS             <1..510
                     /locus_tag="JQH65_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HAY6141318.1"
                     /translation="LSAGYLQPHTDMPGSDDLKGINVKYRYEFTDTLGLVTSFSYANA
                     EDEQKTHYSDTRWHEDSVRNRWFSVMAGPSVRVNEWFSAYAMAGVAYSRVSTFSGDYL
                     RVTDNKGKTHDVLTGSDDNRHSNTSLAWGAGVQFNPTESVAIDLAYEGSGSGDWRTDG
                     FIVGVGYKF"
     gene            574..>1044
                     /locus_tag="JQH65_004121"
     CDS             574..>1044
                     /locus_tag="JQH65_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY6141319.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVVQAQQSAGAAAGNAQQTAQDVAAAATARDDAQRFAENARQDATVTAE"
BASE COUNT          233 a          245 c          344 g          222 t
ORIGIN      
        1 ctgagcgccg ggtatcttca gccccatact gatatgccag gcagcgatga cctgaagggc
       61 attaacgtga aataccgtta tgagtttacg gacacgttgg ggctggtgac gtcattcagc
      121 tatgccaacg ctgaagatga gcaaaaaacg cattacagcg atacccgctg gcatgaggat
      181 tccgtgcgta accgctggtt cagcgtgatg gcggggccgt ctgtgcgcgt gaatgaatgg
      241 ttcagcgcgt atgcgatggc gggcgtggct tacagccgtg tgtcgacctt ctccggggat
      301 tatctccgcg taactgacaa caaggggaaa acgcacgatg tgctgaccgg gagtgatgac
      361 aaccgccaca gcaacacgtc tctggcgtgg ggggctggcg tgcagtttaa cccgaccgaa
      421 tccgtggcca ttgaccttgc ttatgaaggt tccggcagtg gcgactggcg cactgacggt
      481 ttcatcgtgg gtgtcggtta taagttctga ttagccaggt aacacagtgt tatgacagcc
      541 cgctggttca ggcgggcttt ttgtggggtg aatatggcag taaagatttc aggtgtactg
      601 aaagatgggg cgggtaaacc tgtcgtaaat tgtgcgattg aactgcgggc cagaagaacc
      661 agtccgaccg ttgtggcaca cgttgttgcc acttgcgtga cggacaataa cggtgcttat
      721 gtgattgagg ctgagccggg gtattacgag gttgcgcttc actgtaacgg ctggcagcca
      781 acccgtgtcg gggatattga tgtggcaccg actgatgcac cggggacact gaacgcgttt
      841 ctgaatgcac caaaggatgg tgatttacgt ccggaggtga tgaagcgctt tgaggaaatg
      901 gtggtgcagg cgcagcagag cgccggggcc gcagccggaa acgcacagca gacggcgcag
      961 gatgtggcgg cagccgcaac ggcccgtgat gatgcacaac gttttgcgga gaatgccaga
     1021 caggatgcaa ccgtcacagc tgag
//
LOCUS       DADLFA010000350         1031 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.352, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000350 DADLFA010000000
VERSION     DADLFA010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.352"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..465
                     /locus_tag="JQH65_004122"
     CDS             <1..465
                     /locus_tag="JQH65_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014837283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141320.1"
                     /translation="LPISAADKRRFYYYFQEKNPPNIERFFVFDSSEYRYALNMREVV
                     FHQFLSDGLRPIVDEDDDAYEDDYFNVHITLVNGGPVIPLSVEPDAPQNEETDDIGQL
                     NAFFDALDCEPETTDRFMITDEDGEDAFIRIGSIAMVRVALDVLEPVEDDDE"
BASE COUNT          257 a          209 c          254 g          311 t
ORIGIN      
        1 ttgcccattt cagcagcgga taagcggagg ttttattatt attttcagga aaaaaacccc
       61 ccaaacatag aacgcttctt cgtttttgat tcttccgagt atcgttatgc gttaaatatg
      121 cgtgaagtgg tatttcatca gttcctttct gatggtctgc ggccaatcgt tgacgaagac
      181 gatgatgcct acgaagacga ttattttaat gtccatatca cgctggtaaa cggcggacca
      241 gtgattccac tgagtgttga gccagatgcg ccgcaaaacg aagagacgga tgatatcggc
      301 cagttgaatg cattttttga cgcgctggat tgcgagcctg aaaccacgga tcgttttatg
      361 ataaccgacg aagatggaga agatgctttc attcgtattg gttctatcgc tatggtgcga
      421 gttgcgcttg atgtgctgga accagtggaa gacgatgatg agtagtattg aatggattga
      481 tttcatattg ttgagtctgg aatcgcggtg cggccttcgc accacaccgt atgcaaatcg
      541 agtcgctcaa gagcggcttt ttttgtgtct gaaatcaccc atatccgtgc agtatatagg
      601 cctgccagaa aagcagttca gtattcttca ttttgtagtc gatggtccca agtttgctct
      661 ggctggaagc tgcatcgtcc acattacgtg ggcagggtgg tttcgctatg ctacttgaac
      721 gttttgacca tgtggctcag gtagctaata ccaaaagacg tcattttgtc tccacgccca
      781 ccttggtgaa cattcaataa atagtttcgg tcgagagcag ttatgtaggg ctagcagacg
      841 agatgcgcag acatcttgtt gaagacaatt ttactgacca cctgagaacg ctattctacc
      901 gaataatatt tattatcttg gcgaataatg atgattacct gtgtaactat ggttttatgc
      961 tgatgggacc ggcagtacaa tttctgtagt gtactcgcaa tttcaggtct ctggcgttta
     1021 ttctggtgca a
//
LOCUS       DADLFA010000351         1020 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.353, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000351 DADLFA010000000
VERSION     DADLFA010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.353"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(1..912)
                     /locus_tag="JQH65_004123"
     CDS             complement(1..912)
                     /locus_tag="JQH65_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141321.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNNFFNNIQHAFKKKDWV
                     SNYDSLLTLWEFFRCATQIDKDSYQVLSSKNETVKAMDKFLISFSLKDNGAEYTMTLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYAWDILQKKQSDIPKRIKGYIHQ
                     AIGDVSLGYALLEDIVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAVDS
                     DITEEEICEKYKSTIEAFKKEQLALPV"
BASE COUNT          312 a          210 c          142 g          356 t
ORIGIN      
        1 tcatactggt aacgctagtt gctctttttt aaacgcctca atggttgatt tatatttctc
       61 gcaaatctcc tcttcggtaa tatcactatc tacggccaga ttcgcactta ctatcgcatc
      121 cacttgttcg gctaattcga cttgttggca acctgcatta accagaaaat tataaataag
      181 agagtaatct tcatcctcca aaacaacaga ggaaccatta cgaaaacgtt catcccctcc
      241 atagatttta agcgcaaagc ccaaagattc tatgactatg gcaaaattat catcaattat
      301 tttttcgtaa atataccatc catcgcattc tttaattcca cctcctggta tttggagttc
      361 aaatttaccc cgttttaatt tgcttactat atcctccaat aaggcataac ctaaagacac
      421 gtcaccaatc gcctggtgaa tataaccttt aatccttttc ggtatgtcag actgtttttt
      481 ctgcagaata tcccaagcat acaatcttct gttttgttct aacggaaatt cacggttttt
      541 gaaatccatg aatgagttat actcatttat tgttatgggg atttcttcgt attcaaatcc
      601 actgcctcgc aatgtcattg tatattcggc accgttatct ttaaggctga aactgataag
      661 aaacttatcc atggctttaa cagtttcatt ttttgatgaa agcacttgat aactatcttt
      721 atcaatctga gttgcgcaac gaaaaaactc ccacaaagtt aacaggctat cataattcga
      781 aacccaatct tttttcttaa atgcatgctg aatattatta aaaaaattgt ttgaatgtgt
      841 ttttgtatgc gatgatttta cgaataactc caccgcacca ataaaattat tgtgtctgat
      901 cctgggtaac atatggctac cttattttat ttggttattt tatgtcgcca ttaaatatct
      961 cattatagaa gtaaactatc aaaaaacgct cattattaaa atgtaactta ttagcaattg
//
LOCUS       DADLFA010000352         1018 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.354, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000352 DADLFA010000000
VERSION     DADLFA010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.354"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..767)
                     /gene="ipaH3"
                     /locus_tag="JQH65_004124"
     CDS             complement(<1..767)
                     /gene="ipaH3"
                     /locus_tag="JQH65_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HAY6141322.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNHLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITHIPENILSLDPTCTIILEDNPLSSRIRESLSQQTA"
BASE COUNT          289 a          163 c          249 g          317 t
ORIGIN      
        1 tgggcggttt gttgcgacag agactccctg atccgtgagg acagaggatt gtcttcgagg
       61 atgatagtgc aggtcggatc aaggctaagt atattttccg gaatgtgtgt gatgcgattc
      121 tcgcggcacc ggaaaaatat ctcggtttcc tctgagtgat gatttcttac aggtacggct
      181 ggtaggcttt ccagaagatt agtacttaca tcgagcgctt ccagtgattc aggtaactca
      241 gggagaaatg tcagctggtt atttcttact gagagcactt ccagcgatgt aggtaattca
      301 ggaagcatgg ttagctgatt gttatctgca ttaatatatt ccagcaatgc aggcaattca
      361 ggaagcatgg ttagttggtt gttatctaca tcaagatgtt tcagagatgc gggtaattca
      421 ggaagtgttg acaggtgatt gtcacaggcg tcaaggtatt ccagcgatgc tggcaattct
      481 ggtaatgata ttagggcatt ctgagtaatt tccagaacag tgatttgagg tggtaagttg
      541 tcaggtagcg aggacagatt taaacgattc agttgaagct cactgaactg attgatgaga
      601 cattctttaa gtagggagac cgcttcattc cgattttcac cggggagcgc ttgtttttcc
      661 catttatccc atgctgaaaa gtaatcagca tatgtaccgg aaatagtgtt ataaaaagaa
      721 ttttgggaca atgaaaagtt attatttatc ggtaacatga tggacattca gttaaagccg
      781 atggacaaat ggctttgtta aaagaaataa gtaggagttt ttatgaagtt cattccatga
      841 aatgaacttc agcacttttt tattataaaa ggacaaacaa aattggctgt aaaaaaacga
      901 tactgtttca gttaactgtg aatagcttgc ttgcaccgta ttgttcttgt ttttcggtta
      961 gttaaacatc gtacttcata tttgaacgtt ctgccggaat gcattatcaa tagaggta
//
LOCUS       DADLFA010000353         1014 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.355, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000353 DADLFA010000000
VERSION     DADLFA010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.355"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..150)
                     /locus_tag="JQH65_004125"
     CDS             complement(<1..150)
                     /locus_tag="JQH65_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141323.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAP"
     gene            complement(147..350)
                     /locus_tag="JQH65_004126"
     CDS             complement(147..350)
                     /locus_tag="JQH65_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HAY6141324.1"
                     /translation="MVERTMAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAIT
                     PYTLMQAIAAEGDVVVSGATEPE"
     gene            596..931
                     /gene="iraM"
                     /locus_tag="JQH65_004127"
     CDS             596..931
                     /gene="iraM"
                     /locus_tag="JQH65_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HAY6141325.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
BASE COUNT          336 a          236 c          172 g          270 t
ORIGIN      
        1 cggtgcattt atctccagca ccagactttc tatctcaacg ccatacgttg catttttggt
       61 aatatccgtc agcgtcagtg catttagtcc cactgccaga ctgtctttta tggcctggaa
      121 tgccgggcca gtacgatgac gtagtatcac tccggctcag ttgcaccact gaccaccaca
      181 tcaccttctg ctgcaatcgc ctgcatcagg gtataagggg ttatggccac cggactacca
      241 aacggctgcc agccctcttt cagtttatgt gtcagctttt ccgcaagatc tgacggcggc
      301 gccgccctga caacatcata gtgtttaaat gccatggttc tttccaccat ctgaaaaata
      361 attctttaaa atacctgaca tgtaatacag aaaaaacaca aaaccatacc ttaaataaaa
      421 acctgattat caagcagata tgcatggata aactacaaga cgagatataa accaccctgc
      481 atttaaataa acaataaaca acatcagaaa aataattctg ctctatggtt tacattcaaa
      541 aatatcattt atacttttca gaacatcacc agcaaggcat aaacaaggaa actaaatgaa
      601 gtggattgtg attgatacag ttatccagcc atcatgcgga atatcttttt cagtcatatg
      661 gagtaaaata aaattaataa tctggtatca atcggatgct ttcttacctc ctgaaagtat
      721 atttacactg actcacacag gcatcatgct caataacaaa gtgctacctg taaccattta
      781 caacgtagta ccattcaata aaacattctg gaatttaatc aaaaacagcc aggaatgccc
      841 tacaaataca gataacgtat tgaatgaatg ctttaataac cgttgcactc tgcaaatatg
      901 tccttatgga ctaaaacaac aaagtccata aggagtttac tcacatctga caaaatcaat
      961 ataaacagcc cctccggaga ggggctggag agtggcgcta tgtgccattg catg
//
LOCUS       DADLFA010000354         1007 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.356, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000354 DADLFA010000000
VERSION     DADLFA010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.356"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..>1007
                     /locus_tag="JQH65_004128"
     CDS             <1..>1007
                     /locus_tag="JQH65_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAY6141326.1"
                     /translation="VEERQRGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETA
                     RLRYGYSSMTTPDTLFELDMDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPV
                     SLVYHRKHFRKGHNPLLVYGYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELG
                     QQWYEDGKFLKKKNTFNDYLDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPE
                     LFHGVIAQVPFVDVVTTMLDESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQA
                     YPHLLVTTGLHDSQVQYWEPAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYE
                     G"
BASE COUNT          244 a          223 c          267 g          273 t
ORIGIN      
        1 ggttgaagag cgtcagcgcg ggttaaccag tttgcgccaa attaaccgca agacccggga
       61 agtcattggt attgcctttg atgatccagc ctatgtgacc tggattgcct acaatccaga
      121 acctgaaacc gcgcgattgc gttatggtta ttcttccatg accacaccag acactttgtt
      181 tgaactggat atggataccg gtgagcgtcg tgtattaaaa caaacggaag ttcctggttt
      241 tgatgcggcg aattaccgca gtgaacacct gtggatagtc gcccgtgatg gcgtcgaagt
      301 tccggtttcg ttggtctacc atcgcaaaca ttttcgcaaa ggacacaacc cgttgctggt
      361 gtatggctat ggttcttacg gcgcaagtat tgatgccgat ttcagtttta gccgcttgag
      421 tttgttagat cgtggctttg tctacgccat tgtccatgtt cgcggcggtg gtgagctggg
      481 gcaacaatgg tacgaagacg gaaaatttct gaagaagaaa aatacgttta atgattatct
      541 tgatgcctgc gatgcattgt taaaactggg ctatggctct ccttcgcttt gttatgcgat
      601 gggcgggagt gcggggggca tgttgatggg cgttgcaatt aatcaacgcc cggaattatt
      661 ccacggcgtt atcgcccagg taccgtttgt tgatgttgta acaacgatgc ttgatgaatc
      721 aattcctctt accactggtg agtttgaaga gtggggtaac ccgcaggatc cgcaatatta
      781 cgagtacatg aaaagctaca gcccgtatga caacgtcacc gcacaggctt atccgcattt
      841 actggtaacg accggtttac acgattctca ggtgcaatat tgggaaccgg caaaatgggt
      901 cgctaaattg cgcgagctga aaaccgataa ccatctttta ttgctctgta ccgacatgga
      961 ctcaggccat ggcggtaaat ctggccgctt taaatcgtac gaaggcg
//
LOCUS       DADLFA010000355          962 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.357, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000355 DADLFA010000000
VERSION     DADLFA010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..962
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.357"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..790)
                     /locus_tag="JQH65_004129"
     CDS             complement(<1..790)
                     /locus_tag="JQH65_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY6141327.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTL"
BASE COUNT          272 a          152 c          270 g          268 t
ORIGIN      
        1 gcagagtgcg agtcgacaat ggattgttat aaatattaac atctgcataa atgtgcagat
       61 tcatgatact gtctggcaat gtgttcagct gattgttagg gatatatagt gattgcagac
      121 gttgtggaag ttcgggcagt ctgtgtagtt ggttttcccc aacgtttaaa agcctcagat
      181 tttgaggaag gggcggtagc tcttgtaatc tgttatcaaa agcacttagt tcctgtagtt
      241 cacatggtaa ttctggtaag gcagtaagct ggttgttgcc aacagaaagt ttttgtagag
      301 ccgggggaag atcaggtagc gtctccaggc tattgagaag ggctgagagt gactgcaagg
      361 aagatggaag agaaggtaaa catgttaaaa ctctgttaag tgaaacatca agagcgacca
      421 ggtgaggcgg aagagccggg agcctgctca gtctgttatc gctggcttta agtacagtta
      481 aggacggagg gagttctggc agagagcgta gctcattgct agagatgtta agttcttgta
      541 tgtgcggggg caggtatggg agagagcgta atctgagtaa acttaaattg agggctggct
      601 cttgaaaagc cagacatatt ttcagtaatc gaactgcctg tgttcggtct tctgttgcag
      661 caccttcctt ggcccagtta tcccagatgc ggtgataatc cgtgatattt tgctcttcta
      721 cggataaacg ggatatacat cctaaaccac aaaaggagcg gaaaaaagaa ggattgtggg
      781 caggtttcat ttattattga tcctcattgt ttatatatta atatgttcgt gcaaatggag
      841 tgaggattct ttaaaaaaac gctcctgtaa atagttaaga acaggaagaa ctgttgggga
      901 tgtggctggc cgaaaatgaa ggggtgaagt tctggctcaa tgtgctgact gaactgaaaa
      961 aa
//
LOCUS       DADLFA010000356          953 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.358, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000356 DADLFA010000000
VERSION     DADLFA010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..953
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.358"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            1..534
                     /locus_tag="JQH65_004130"
                     /pseudo
     CDS             1..534
                     /locus_tag="JQH65_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          281 a          215 c          203 g          254 t
ORIGIN      
        1 cagttgtcca ggccttccga aatcaaccga ctgaaggcgc tggtcgcgaa actgcagcgt
       61 atgcagttcg gtaaaagctc agaaaaactt cgcgcaaaaa ccgaacggcg gatacaggaa
      121 gcacaggagc gaatcagcgc acttcaggag gaaatggcgg aaacactggg tgagcaatat
      181 gacccggtac tgccatcttc cctgcgccag tcttcagccc gtaaaccgtt accagcctca
      241 cttccccgtg ccccccgggt catccggccg gaagaggaat gctgtccggc ctgtggtggt
      301 gaactcagtc cactgggatg tgatgtgtca gagcagctgg agcttatcag cagcgccttt
      361 aaggttatcg aaaaacaacg ccctaaactg gcctgtcgcc ggtgcgacca tatcgtgcag
      421 gcaccggtgc cttcaaaacc cattgcacgc agttatgccg gagcggggct tctggcccat
      481 gttgttaccg gaaagtatgc agaccatctg ccattatacc gccaaagtga tctactattc
      541 cacacagcta tttagctctc tttttgttga gctcccatga ttctttcatt tattgttact
      601 aattatttta tgttatcatg atgttagaac acgctctgct ctgtgtctct attttatcta
      661 tcatattctg aggattaact acataacaag gcgcaagata tatatccaaa aacaaataat
      721 accgttgcaa taattccttt cagtaaagga ggtgtgatta atttcagggg atttcagagt
      781 gaaaattgta caacatattg aaatatgttc atttcgttat aatacacaca agaaaatatt
      841 aagttagata atatattcta aataaccata gataaaaatg cacataatgg ggaatatttt
      901 atgaaaatac ctgaagcagt aaatcatatt aatgttcaga ataatattga tct
//
LOCUS       DADLFA010000357          941 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.359, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000357 DADLFA010000000
VERSION     DADLFA010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..941
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.359"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            5..565
                     /locus_tag="JQH65_004131"
     CDS             5..565
                     /locus_tag="JQH65_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY6141328.1"
                     /translation="MFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAANHSKQQPSK
                     PCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQHYWNLRIVA
                     SSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQLCGYSSTSY
                     FISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(611..687)
                     /locus_tag="JQH65_004132"
     tRNA            complement(611..687)
                     /locus_tag="JQH65_004132"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(651..653),aa:Arg,seq:tct)
BASE COUNT          259 a          227 c          217 g          238 t
ORIGIN      
        1 tctgttgttt ttaaataaga acttaacctg tgtaaagccc tggtcgcggc tggcaacccc
       61 ggttatcgct tgtcatagcc gtacaccgga agtgttccgg ctagccgcca accacagcaa
      121 gcagcaaccc agcaaaccct gcgaagcgga gttgacgcgc gcattacttt ttaccgtatt
      181 gtctaacttt cttgagcaat cgcggtttat tgccctactg atgtatatct tacgcagcag
      241 cgtccgcgac agcgtctgcc gcattattca aagcgatatt cagcattact ggaatctgcg
      301 aattgtcgcc agttcgctat gtttaagccc cagcctgctc aaaaagaaat taaaaaacga
      361 aaataccagc tatagccaga ttgtcacaga gtgtcgtatg cgttacgccg tacagatgtt
      421 attgatggat aacaaaaata tcactcaggt ggcgcaatta tgtggctata gcagcacgtc
      481 gtactttatc tctgttttta aggcgtttta cggcctgaca ccgttgaatt atctcgccaa
      541 acagcgacaa aaagtgatgt ggtgaagggc aaagcggaaa cggataagac gggcataaat
      601 gaagaagaaa tggcgcgccc tgcaggattc gaacctgcgg cccacgactt agaaggtcgt
      661 tgctctatcc aactgagcta agggcgcgtt gataccgcaa tgcggtgtaa tcgcgtgaat
      721 tatacggtca acccttgctg agtcaatggc ttttgatctg gttgctgaac aagtgaacga
      781 ccgcgtctga ttttctgatt tatttcgcta tagcggcaaa caaacgcaca ccgctgcgcg
      841 tctgaatcaa gaaaacccgt attttcatgt atcaaagtac aatttaccgc cctaacggaa
      901 aattgtccgc tcctatgaga ctggtaacta tgaaaccaac g
//
LOCUS       DADLFA010000358          928 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.360, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000358 DADLFA010000000
VERSION     DADLFA010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..928
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.360"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(45..381)
                     /locus_tag="JQH65_004133"
                     /pseudo
     CDS             complement(45..381)
                     /locus_tag="JQH65_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          305 a          167 c          168 g          288 t
ORIGIN      
        1 aatatgaaaa gatggtttaa ataccccgtt acttttgact tacactatac ggtatcgcat
       61 cgtttaatat tcgcaccggc cagattttta tttctattag ttgtcacaat actgaatgcg
      121 tacgaccaca gtattctggc tcctgtgtgg tcatgcttta attctgcgtt ccggacagat
      181 aagcagttgc ttgcaggaat ccttcttgtg ttaatgtcag ttcccctttt taccagtgct
      241 gatttccaca ttccgtccaa cagagcttat agcctttccc tggattatag cattgtccgg
      301 ctgaagttct ttttgaataa taatagaagc actgctggca gatccagtcc gtttttcata
      361 acccactgta ctgataacca taatctaatc agtagaaatt gagtcgaaaa taagcactac
      421 tccatacagg ataattagag gtcagtttga ttattcacaa ttcattatca gcattttctg
      481 tttctgacga aatcaacatg aaaagaacca tatatgataa ttattataat aacggcttta
      541 attggaatac atatattaca acgtattata tataattggt attctgggaa ctatattctc
      601 aaaatacagt agaaacgagg tatgtttctg gtggaaagga cagtgggatt aaaaagtaag
      661 aattgataaa aaaacgccag caacacatag tgccggcgga gggaataccc catggagaaa
      721 atgtgatgcc tagagcacca tgaatatata attaaaaata gttagagttg tcaatacttt
      781 cacaaaaata attttcgtag tttcgtagta tagaaagata tttttatgca tgacctacct
      841 gaatttgctc cgggtagagg ttataaataa aaattgaatc acgacaaaca caatattcat
      901 agtatggcga tgcctacgcc agcaagaa
//
LOCUS       DADLFA010000359          920 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.361, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000359 DADLFA010000000
VERSION     DADLFA010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..920
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.361"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..>920)
                     /locus_tag="JQH65_004134"
     CDS             complement(<1..>920)
                     /locus_tag="JQH65_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HAY6141329.1"
                     /translation="RFSYGITGGNVFFSCLVFVFFYFAILMTVSADMPLVGCVLTLSL
                     LVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMAIGMGTVFFIST
                     KMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFKVKLDT
                     AYCIKYAILAFLALLPFLAVAGYIIFEQILNAYDSSVYANDDIENLQQFMEMQRKMII
                     AQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCALVVISG
                     ITGGLAYPLLKIWMIDWQAKNTYLLGDLDDL"
BASE COUNT          353 a          207 c          141 g          219 t
ORIGIN      
        1 aggtcatcca aatcgcccag caaatacgta ttttttgcct gccagtcaat catccatatt
       61 ttcagcagtg gataagccag accgcccgta atcccggata tcactaccaa cgcacacatg
      121 cgataaagca taccgtggta cgttaaagtt gagcgaaaac gaatacgccc atcattcagt
      181 gacaggttac tcataaaatg atttcgcaac gacaccgtta aataactggt gctaacagca
      241 atcccaaaat aatagattaa ctgcgcgatt atcattttac gttgcatttc cataaattgc
      301 tgtaaattct caatatcatc atttgcatac acagaactat catacgcatt taatatttgt
      361 tcgaagataa tataaccagc aacagcgaga aaaggtaata aagctaaaaa tgcgaggatg
      421 gcatatttta tacaatacgc agtatctaat ttcaccttga aacgatgtat accgaaactg
      481 gtattgctcc agagaaaact cattaccagg ctatataaag taccattaaa aataccaatg
      541 gaaacaatac caactattgc catcagaacc acagatataa taacactact tgaactattg
      601 gcatgtagca tctttgtcga gataaagaaa acagtcccca tcccaatggc cattaaaatc
      661 ggcaagaaaa aggttaccca ccagaaccct ttcatagagc aattaaaact aaatcttacg
      721 ccgttgagac tagtcatcaa ggcctgataa cgcagtcctt ttgctgccat aaatataagc
      781 aaaaccaaca gtgacaaagt caaaacacag ccaacaagcg gcatatctgc tgacactgtc
      841 attaagattg cgaaatagaa aaaaacaaaa acaagacaac taaaaaaaac attcccacca
      901 gtaattccat aagaaaatcg
//
LOCUS       DADLFA010000360          916 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.362, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000360 DADLFA010000000
VERSION     DADLFA010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.362"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            1..624
                     /locus_tag="JQH65_004135"
                     /pseudo
     CDS             1..624
                     /locus_tag="JQH65_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
     gene            675..>916
                     /gene="chbA"
                     /locus_tag="JQH65_004136"
     CDS             675..>916
                     /gene="chbA"
                     /locus_tag="JQH65_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HAY6141330.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGK"
BASE COUNT          221 a          224 c          252 g          219 t
ORIGIN      
        1 gttcttcggt attcatggcg cgctggcgct gaccgcactg gacaacggca ttatgacgct
       61 gtgggcgctg gaaaatatcg cgacctatca gcaatatggt tccgtcgaag cggcgctggc
      121 agccggtaag accttccata tctgggccaa gccgatgctg gactccttta ttttccttgg
      181 ggcagtggtg cgactttagg cctgatcctg gctatcttta tcgcctctcg ccgtgctgat
      241 tatcgtcagg tggcaaaact ggcgctgccg tccggcatct tccagattaa cgaaccgatt
      301 ctgtttggtc tgccaattat catgaacccg gtgatgttta tcccgtttgt actggtacaa
      361 ccgattctgg cggcaatcac cctcgcagcg tactacatgg gcattattcc tccggtgacc
      421 aatattgcac cgtggaccat gccaaccggt ctgggagcct tctttaacac caacggtagc
      481 gtcgctgcat tgctggtcgc actcttcaac cttggtatcg caacgttaat ttatctgccc
      541 tttgttgtgg tggctaacaa agcgcaaaac gcgattgata aagaagagag cgaagaagat
      601 atcgctaacg ccctgaaatt ttaatcgatg ccggtacggt taatcgtgcc ggtaagaaga
      661 gagaggaacg atgtatgatg gatctcgata acattcccga tacgcaaaca gaagctgaag
      721 agctggaaga agtggtgatg gggcttatca tcaactccgg acaagcgcgc agcctggcgt
      781 atgcggcact gaaacaggcg aagcagggcg attttgccgc agcaaaagcc atgatggatc
      841 agtcacggat ggcattgaat gaagcgcatc tggtacagac gaaactgatt gaaggcgatg
      901 cgggcgaagg taagat
//
LOCUS       DADLFA010000361          915 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.363, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000361 DADLFA010000000
VERSION     DADLFA010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..915
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.363"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..186)
                     /locus_tag="JQH65_004137"
     CDS             complement(<1..186)
                     /locus_tag="JQH65_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAY6141331.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIRE"
     gene            complement(479..553)
                     /locus_tag="JQH65_004138"
     tRNA            complement(479..553)
                     /locus_tag="JQH65_004138"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(518..520),aa:Gly,seq:tcc)
     gene            complement(557..632)
                     /locus_tag="JQH65_004139"
     tRNA            complement(557..632)
                     /locus_tag="JQH65_004139"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(597..599),aa:Thr,seq:tgt)
     gene            complement(635..711)
                     /locus_tag="JQH65_004140"
     tRNA            complement(635..711)
                     /locus_tag="JQH65_004140"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(675..677),aa:Arg,seq:tct)
     gene            complement(713..789)
                     /locus_tag="JQH65_004141"
     tRNA            complement(713..789)
                     /locus_tag="JQH65_004141"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(753..755),aa:Ile,seq:cat)
BASE COUNT          267 a          234 c          212 g          202 t
ORIGIN      
        1 ttctcttatt ttgaaataca gattcgtcag ataagtcaga agccccagaa gcagacttcc
       61 cagcacacca atcgcagccc actgtgatgg actgacctga tccaaccact gcaaaaacca
      121 gtagccggca ctgccggcgg aggtgccgta ggcaatgccc gttgaaattt tgtccatgga
      181 tttcatagcc tcacctccgc aaataacgga tggcgtagtt ttacactgag aaatgaaagg
      241 gatttgaaaa gaaaaaaacg caaaagcggg cgaaacgata tatacagtaa ggaaagcact
      301 ctatccaaca aaccacccac agttaatcgg aataaaagca gagtgcttat gaatgatcgc
      361 acgcccgaag gttagtattt ctgcacagca attttgcaaa aaaaggcgat cattcataac
      421 ttaaacgtct ttcagtcact ccgggatttc ccatcatcgc agactgaaag actctgactg
      481 gagcgggcag cgggaatcga acccgcatca tcagcttgga aggctgaggt aatagccatt
      541 atacgatgcc cgcatatggt gccgactacc ggaatcgaac tggtgaccta ctgattacaa
      601 gtcagttgct ctgcctgctg agctaagtcg gcgctggccc accaccgagg actcgaacct
      661 cgcaccgtca acttagaagg ttgatgctct atccggatga gctagtggtg gttggtggcc
      721 cttgctggac ttgaaccagc gacctggcga ttatgagtcg ctcgctctga ccaactgagc
      781 taaagggccg gaggcagaat aataaccata tgtcatcaca tctgcaaact catctgacca
      841 ccagcacgtt taacgtcctg tgccgttttt caggtataaa aaaacccgca taaagcgggc
      901 tctttcaaat gtcca
//
LOCUS       DADLFA010000362          909 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.364, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000362 DADLFA010000000
VERSION     DADLFA010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..909
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.364"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..>909)
                     /locus_tag="JQH65_004142"
     CDS             complement(<1..>909)
                     /locus_tag="JQH65_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY6141332.1"
                     /translation="AFLTGIAGALQTPTLSIFLADELKARPIMVGFFFTGSAIMGILV
                     SQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNRNYFILLSTGVLLSSFASTANPQ
                     MFALAREHADRTGRETVMFSTFLRAQISLAWVIGPPLAYELAMGFSFKVMYLTAAIAF
                     VVCGLIVWLFLPSIQRNIPVVTQPVEILPSTHRKRDTRLLFVVCSMMWAANNLYMINM
                     PLFIIDELHLTDKLAGEMIGIAAGLEIPMMLIAGYYMKRIGKRLLMLIAIVSGMCFYA
                     SVLMATTPAVELELQILNAIFLGILCG"
BASE COUNT          280 a          214 c          213 g          202 t
ORIGIN      
        1 ccacagagaa taccaaggaa gatggcattt agaatttgca attccagctc aaccgccgga
       61 gtcgtcgcca tgagtacgct ggcgtaaaaa cacataccac tcacgatagc aatgagcatt
      121 aatagtcgct tgccaatacg tttcatgtaa tagcctgcga ttaacatcat cggaatttcc
      181 agaccggcag cgataccaat catttctcca gccagtttat cggttagatg cagttcatca
      241 ataataaata gcggcatatt tatcatgtag agattattcg ccgcccacat cattgaacag
      301 accacaaaaa gtagccgcgt atcccgcttc ctatgggtgg agggtaaaat ttctacgggt
      361 tgggtaacga caggaatatt tctttgtatt gatggcaaaa acaaccagac tatcagcccg
      421 caaacaacaa atgcgatggc agcggtgaga tacatcactt taaaactaaa ccccattgcc
      481 agttcataag cgagcggtgg cccgataacc caggcaagcg agatctgagc acgtaaaaat
      541 gtactgaaca tgaccgtctc acggcctgtt ctgtcggcgt gttcacgggc gagggcgaac
      601 atttgcgggt ttgcggtaga agcaaaacta ctcagaagta cgcccgtaga gaggagaatg
      661 aagtagttgc gattccacgc aaaaagcgtg caggccagca ctccaaataa gcaacatagc
      721 agaatcagta atttacggtc gccttgttta tcggagtgcc ttgccagaaa ttgactgacc
      781 agaattccca taatagcgct accggtgaag aaaaaaccta ccattatagg acgggctttc
      841 agttcatctg cgaggaatat acttagggta ggagtctgaa gagcgcccgc aatacccgtc
      901 agaaaggcg
//
LOCUS       DADLFA010000363          894 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.365, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000363 DADLFA010000000
VERSION     DADLFA010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.365"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(136..801)
                     /locus_tag="JQH65_004143"
     CDS             complement(136..801)
                     /locus_tag="JQH65_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAY6141333.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT          226 a          228 c          201 g          239 t
ORIGIN      
        1 aactggggct tttctctatc tgccttttgg tgttcatgcc tgaagcagat agcctgaagc
       61 agatagcctc aagcacccgc agtcattcta cttacctcgc caatatgaaa tcaatcagaa
      121 aggtgcccca taaaatcact ccttctcttc tttaccgtag tggagttgac caattttgat
      181 aagagggcgt ccctgagatt tgcggtgtag attggtatcg cgcagagaat acacacagcc
      241 acaatattcc tgctgataga atttttcgcg cttgctgatt tcaatcatac gggacgagcc
      301 tccctgcttg cgccagttat aatcccagta caccatgccc ggataatgcg cgacggctcg
      361 ccgcccacag tcgttaacct gctgcatatt tttccagcgt gaaatgccca gtgaactgct
      421 gatcacactg aaaccatttt cagcggcgta cagcgctgtc cgctcaaaac gcatgtcaaa
      481 acacatggtg caacggatcc ctcgttcggg ctcccattcc attcctttgg ctcgttcaaa
      541 ccagttgtct gtgtcgtaat cagcatcaat aaacggcacg ccgtgttgtt cagcaaagcg
      601 aatattctca tccttacgaa ttaaatactc tttctgagga tgaatgttcg ggttgtagaa
      661 aaagatggtg tagtcgattc ccgaggcctg aagcgcctcc atcacctcac cggaacatgg
      721 agcacagcaa gagtgcagta gtagtttgtt tgccccgttt gggagctcca atttgggccg
      781 tttgaaatca gcaactgtca taaatatgtt tattggggtc ataaaaatat caaagagtgt
      841 agcattagaa cggggctatc ggaaacagat gtgtgactgc tccccgccct ttcg
//
LOCUS       DADLFA010000364          867 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.366, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000364 DADLFA010000000
VERSION     DADLFA010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.366"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..362
                     /locus_tag="JQH65_004144"
     CDS             <1..362
                     /locus_tag="JQH65_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141334.1"
                     /translation="TPVALSANDPANVWGNLLPWPAHPATLVPTRRAGALVVVSSGKL
                     LLYLAQGGKKMLVWQEKEELLAPEVFHALTTALRREPRLRFTLTEVNDLPVRQTPMFT
                     LLREAGFSSSPQGLDWG"
     gene            complement(413..760)
                     /gene="grxD"
                     /locus_tag="JQH65_004145"
     CDS             complement(413..760)
                     /gene="grxD"
                     /locus_tag="JQH65_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HAY6141335.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
BASE COUNT          188 a          227 c          233 g          219 t
ORIGIN      
        1 atacaccagt ggcgctctct gccaacgatc cggctaatgt gtggggaaat cttctgccct
       61 ggcctgcaca tccggcaacg ctggttccaa cgcgtcgggc gggtgcgctg gtggtcgttt
      121 ctagcggcaa attgttactc tatctggcgc aaggtggcaa aaaaatgctg gtctggcagg
      181 aaaaagagga attactcgcc ccagaggttt tccacgcgct gactaccgca ctgcgtcgcg
      241 aaccacggct gcgctttacg ctaacagaag tgaatgatct accggtccgg caaacgccga
      301 tgtttacgct gctgcgtgag gcgggatttt caagttcgcc acaagggctg gattggggat
      361 agagaaagga ctgacggatg cccgttcgca tccgtcagta ttgcaggacg gattattccg
      421 cgtccggctc ttcagacttg tatttagcgg cagtttcttt gatcagctgc tgcagttcgc
      481 cacgctgata catttcgatc acgatatcac aaccgccgac cagctcgccg tcaacccaca
      541 gttgcgggaa ggtcggccag ttagcatatt tcggcagttc cgcacgaatg tccggattct
      601 gcagaatatc aacataggca aaacgttcgc cacatgcggc aagcgcctgg actgcctggg
      661 cagagaaacc gcagctcggc agtttcggtg aacctttcat gtacagcagg atcgggtttt
      721 cagcaatctg gcgttggatt ttttcgatag tggtgctcat tgtcttgctt cctcaaactt
      781 ctttacggca gtaatctgac attgtagcgg gtcagtgcgg catcggaaaa taacattttc
      841 atcacgcttt tgctatttta tcccttt
//
LOCUS       DADLFA010000365          840 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.367, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000365 DADLFA010000000
VERSION     DADLFA010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..840
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.367"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..>840
                     /locus_tag="JQH65_004146"
     CDS             <1..>840
                     /locus_tag="JQH65_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAY6141336.1"
                     /translation="LVGYIAWLATAYRMSFSREINLGLAQHEFELFCQPLLNARSQQC
                     IGVEILLRWNNPRQGRISPDVFIPIAEEHHLIVPLTRYVMAETIRQRHVFPMSSQFHV
                     GINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITERDALLDVDYRIARELHRKNVK
                     LAIDDFGTGNSSFSWLEKLRPDVLKIDKSFTAAIGSDAVNSTVTDIIIALGQRLNIEL
                     VAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFPKWLAGSQPPPARHNGHITPIM
                     PLR"
BASE COUNT          195 a          213 c          217 g          215 t
ORIGIN      
        1 actggtgggc tacatcgcct ggctggcgac cgcttaccgg atgagctttt cccgcgaaat
       61 caatctgggc ctggcgcaac atgagttcga attgttctgt cagcctttgc ttaatgcgcg
      121 cagccagcaa tgtattggtg tagagatttt gctgcgctgg aacaatccgc gtcagggccg
      181 gatttcaccg gatgtgttta ttcctatcgc ggaagaacat catttaattg tgccactgac
      241 ccgctatgtg atggcagaaa ccattcgtca gcgccatgtt ttcccgatga gtagtcagtt
      301 tcatgttggc attaacgtcg cacccagcca ttttcgccgt ggtgtgctga taaaagatct
      361 caatcagtac tggtttagcg ctcacccgat tcagcaactg atcctcgaaa tcaccgaacg
      421 cgatgcctta ctggatgttg attatcggat tgcccgcgag ctacatcgta aaaacgtcaa
      481 actggcgatt gatgacttcg gcaccggcaa cagttcattt tcctggcttg aaaaattacg
      541 tcctgacgtg ctgaaaattg ataagtcatt taccgcagct ataggttctg acgcggttaa
      601 ctcgacggtg accgatatca tcatcgcgct ggggcaaaga ctgaatattg aactggtggc
      661 ggagggcgtg gaaacgcaag aacaggcgaa gtatttgcgc cgtcatggcg tgcatatttt
      721 gcaagggtat ttgtacgcac agccgatgcc gctacgcgat tttcccaaat ggctggcggg
      781 cagccaaccg ccgcccgccc ggcataatgg acatatcacg cccattatgc cgttacgtta
//
LOCUS       DADLFA010000366          824 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.368, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000366 DADLFA010000000
VERSION     DADLFA010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..824
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.368"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            126..>824
                     /locus_tag="JQH65_004147"
     CDS             126..>824
                     /locus_tag="JQH65_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141337.1"
                     /translation="MLPSISINNTSATYPESINENNNDEVNGLVQELKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRFNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGAPSFIFDKISHKLRHTVTEF
                     DESGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSENARSASA"
BASE COUNT          313 a          149 c          136 g          226 t
ORIGIN      
        1 ccctggctat tgtgcgctca tacactcaaa ttaaagatag gttctaaata aatgagcgtt
       61 ttttgatagt ctatttcatt aggtaatata tatttgtaac aaatcaatca aaatggaata
      121 aaatcatgct accatctatt tcaatcaaca ataccagcgc aacttaccca gaatccatta
      181 atgaaaataa caatgatgaa gttaatggat tagtacaaga gctcaaaaac ctttttaatg
      241 gtaaggaagg aataagcacc tgtattaaac atctacttga gcttataaaa aacgccatac
      301 gagtaaacga cgatccttat agatttaata ttaataattc ctcagttact tatattgata
      361 ttggctccaa tgatacagac catattacta ttggtatcga caaccaagaa ccaatagaat
      421 tacctgcgaa ctataaagac aaagaactcg tccgtactat cattaatgac aacatagttg
      481 agaagactca tgatatcaat aacaaggaaa tgatcttcag cgcattaaaa gaaatatatg
      541 atggagctcc tagttttatc ttcgataaga tatcacacaa actcagacat acggtaacgg
      601 aatttgatga gagcgggaaa agcgaaccaa cggacttatt tacctggtac ggtaaagata
      661 aaaaaggcga ctctctcgct attgtaatta aaaataaaaa cggaaatgat tacttatccc
      721 tcggttacta cgatcaggac gactaccaca ttcaaagagg aattcgtatt aatggtgata
      781 gtctcaccca atattgtagt gaaaacgcca ggagtgcttc agcg
//
LOCUS       DADLFA010000367          811 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.369, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000367 DADLFA010000000
VERSION     DADLFA010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.369"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..346
                     /locus_tag="JQH65_004148"
     CDS             <1..346
                     /locus_tag="JQH65_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141338.1"
                     /translation="VMCLTKPAFSYYIAAYLKLFIENYYEADALVDTILNMLTPPVRN
                     GIPSTTWIAKNLDCFSRNKRVAISFTFQYLCNEYNDPNTEKALNIYWGQYLNESLLIK
                     NVQNSKNKCKSQ"
     gene            complement(407..592)
                     /locus_tag="JQH65_004149"
     CDS             complement(407..592)
                     /locus_tag="JQH65_004149"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141339.1"
                     /translation="MIMVAVAAAVTDALLVFFRQLAPFVVLPALGIFIIHGTVRQAVG
                     FIVMPERDQALMSLTVI"
     gene            591..794
                     /locus_tag="JQH65_004150"
     CDS             591..794
                     /locus_tag="JQH65_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HAY6141340.1"
                     /translation="MMKNQMEPEYTPLRKIHLYHCDHRGLPLALIRSDGRTGWRVEYD
                     EWGNLLSEDNPHRERSSEVHFLY"
BASE COUNT          251 a          169 c          188 g          203 t
ORIGIN      
        1 tgtcatgtgt ctcactaaac cagcattctc atattatatt gcagcctatt taaaactttt
       61 tattgaaaat tattacgaag ctgatgcttt agtggatact attttaaata tgctcactcc
      121 tcctgttagg aatggtatcc ccagcactac atggatagca aaaaatcttg attgctttag
      181 tcgtaataaa agggtggcta tttcttttac attccaatat ttatgtaatg aatataatga
      241 tccaaatact gaaaaggcgt taaatatata ttggggacag tatctgaatg aatcattgct
      301 tattaaaaat gtacagaact ctaaaaataa atgtaaatca cagtaagaca gaaccaagca
      361 gaaatcgtgg gcgtggttcg caccctgacg gagggtgtag cgtgtactag atcacagtga
      421 gcgacatcaa agcttggtca cgttctggca ttacgatgaa gccgaccgcc tgacgcaccg
      481 taccgtgaat gatgaagatg ccgagtgctg gcagtacaac gaacggggct agctgacgga
      541 aaaagacgag cagcgcatcc gtgacggcgg cagccacagc caccattatc atgatgaaaa
      601 accagatgga gccggaatac acgccgctgc gtaaaattca cctgtaccac tgcgaccacc
      661 ggggactgcc gctggcgctg atacgcagtg acggccgaac cggatggcgt gtagagtatg
      721 acgaatgggg caacctgctg agtgaggata acccgcacag ggaaagatca tcagaagttc
      781 actttttgta ctaaataatt cgcattttat g
//
LOCUS       DADLFA010000368          807 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.370, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000368 DADLFA010000000
VERSION     DADLFA010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.370"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..221
                     /locus_tag="JQH65_004151"
     CDS             <1..221
                     /locus_tag="JQH65_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAY6141341.1"
                     /translation="LRRDRPDIAVRYYYKGVLWSPGYFASSCGGAPISVIRQYIEQQQ
                     TPGQVENRALYPRPEGWGFTAHRIKSAI"
     gene            complement(239..807)
                     /locus_tag="JQH65_004152"
                     /pseudo
     CDS             complement(239..807)
                     /locus_tag="JQH65_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
BASE COUNT          203 a          192 c          183 g          229 t
ORIGIN      
        1 tactgcgacg agatcgacca gatattgcag tcaggtatta ctacaaaggc gttttgtgga
       61 gtcctggcta ttttgccagt agctgcggag gtgcgccaat atccgtcatc cgccaataca
      121 ttgaacaaca gcaaacacct ggtcaggtgg aaaaccgcgc cttatatccc cgccctgaag
      181 gatggggttt tacggcgcac cggataaaaa gcgccatcta acgatggcgc gtctggaact
      241 actttttgtt aacgtcaaac atggtggcat cggtcgccat gtcatcaata acttgtttaa
      301 tattttcgaa agccattggc gtactttcgt tattcaggtc tttaccttag ccctgacgaa
      361 cgactttgat aaccggttta ttagtcgctg catcaatcag ctcaccttca aaatagaggc
      421 gagtatccat ggtacggtga cctgtagcca tttgcgtccc cgcaaccact aatgcaacag
      481 gaaccacttc atagaattgc agcccttctt tgctggtatc tacaccggta atggccccac
      541 ggaaaatcag actacgcggc ccagcggtgg taaccagtgg tttacgctgc gctatcgctt
      601 ctttcatttc ggtgttggta tagttcaaaa ttttatccag aactttctgc cctacctggg
      661 tagaaggttt tggcaccgga taataagtga ttgggttcca gacgatgctg tcatatttgc
      721 tttgatcaaa actcgggtct acccaacgta aaacaggttt acctgttgct gaggttgttt
      781 cttttaaatc agagtaattg tttaaaa
//
LOCUS       DADLFA010000369          792 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.371, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000369 DADLFA010000000
VERSION     DADLFA010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.371"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            3..>792
                     /gene="fdrA"
                     /locus_tag="JQH65_004153"
     CDS             3..>792
                     /gene="fdrA"
                     /locus_tag="JQH65_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HAY6141342.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGAGSSQSL
                     TQVRRWDSACQKLSDANLALISVAGEYAAELANQALERNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIAQAGE
                     GITHAIGLGERDLSREVGGISALTALEMLSADEKSEVLAFVSKPP"
BASE COUNT          191 a          181 c          245 g          175 t
ORIGIN      
        1 ttatgatcca cgcctttatt aaaaaagggt gttttcagga ttcggtcagt ttaatgatta
       61 tttcacgaaa actcagcgaa tcagaaaatg ttgatgatgt ttccgtaatg atgggtacgc
      121 ccgccaataa agcgttatta gataccacag gtttctggca tgacgatttt aataacgcca
      181 cgccgaacga tatttgcgtg gcaattcgta gcgaagcggc ggatgcgggg atcgcgcagg
      241 cgattatgca gcagcttgaa gaggcgctaa aacaactggc gcagggggca ggcagcagcc
      301 agtccttgac gcaggtgcgt cgctgggaca gtgcctgtca gaaattatcc gatgccaatc
      361 tggcgctgat ttcagtggct ggcgagtatg cggcggagct ggcgaatcag gcgctggagc
      421 gcaacctcaa cgtgatgatg ttctccgata acgtcacgct ggaagatgaa atccaactta
      481 aaacccgcgc gcgagaaaaa ggcttgctgg tgatggggcc ggactgcggt acgtcgatga
      541 ttgccggcac gccgctggct tttgctaacg tgatgccgga aggcaacatt ggcgtcattg
      601 gcgcttccgg taccgggatt caggaactgt gttcgcagat tgcgcaggca ggggagggaa
      661 ttactcacgc gattggtctt ggcgagcgcg acctcagccg tgaagtaggc ggcatcagtg
      721 cgctgacggc gctggaaatg ctcagtgcag acgagaaaag cgaagtgctg gcatttgttt
      781 caaagccacc tg
//
LOCUS       DADLFA010000370          784 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.372, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000370 DADLFA010000000
VERSION     DADLFA010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..784
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.372"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            6..>784
                     /locus_tag="JQH65_004154"
     CDS             6..>784
                     /locus_tag="JQH65_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HAY6141343.1"
                     /translation="MSKLPTGVEIRGKYIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRALIVHEISSGEFEYLRRFPQSSTGAKMVTTRVIKTFGELCDIWTKIKETELTTNT
                     MKKTKSQLKTLRIIICESTPISHIRYSDILNYRNELLHGETLYLDNPRSNKKGRTVRT
                     VDSYIALLCSLLRFAYQSGFISTKPFEGVKKLQRNRIKPDPLSKTEFNALMESEKGQS
                     QNLWKFAVYSGLRHGELAALAWEDVDFEKGIVNVRRNLTIL"
BASE COUNT          276 a          137 c          180 g          191 t
ORIGIN      
        1 atgatatgtc taaattacca acaggtgtcg agattcgagg taaatacatt cgcatctggt
       61 tcatgtttcg aggaaaacga tgtcgggaaa cattgaaagg ctgggagatt actaacagta
      121 acattaaaaa ggccgggaat ttaagagcgt tgatagttca tgaaatcagt tccggtgaat
      181 ttgagtattt aagacgtttt ccccagtcca gcactggggc aaaaatggtg acaacgaggg
      241 tcataaaaac attcggggag ctttgtgata tctggacaaa aattaaagag acagagttaa
      301 caacaaacac aatgaagaaa acgaaatcac aattaaaaac actcaggata ataatttgtg
      361 agagtacccc gatatcgcat attcgttata gcgatatctt aaactaccgg aatgaactgc
      421 tgcatggaga aacgctttac ctagataatc caagatccaa caaaaaagga agaaccgtgc
      481 gcacagttga tagctatatc gccctgctct gttcgttgtt acgttttgcg tatcagtcgg
      541 gatttatatc aaccaaacca tttgaaggag taaaaaaatt acagcgaaac agaataaaac
      601 ctgacccgtt atctaaaaca gaattcaatg cattaatgga aagtgaaaaa ggacagagcc
      661 aaaacttgtg gaaatttgcc gtttactccg ggcttcgtca cggggaactg gcagctctgg
      721 cgtgggagga tgtggatttc gagaagggaa ttgtgaatgt cagaagaaac ctgacgatac
      781 ttga
//
LOCUS       DADLFA010000371          784 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.373, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000371 DADLFA010000000
VERSION     DADLFA010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..784
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.373"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..>784
                     /locus_tag="JQH65_004155"
     CDS             <1..>784
                     /locus_tag="JQH65_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAY6141344.1"
                     /translation="VHADDIPVPVQEPGSGKTRTARLWVYVRDDRNAGSQMPPAVWFA
                     YSPDRKGIHPQNHLAGYSGVLQADAYGGYRALYESGRITEAACMAHARRKIHDVHARV
                     PTDITTEALQRIGELYTIEAEVRGCSAEQRLAARKARAAPLMQSLYDRIQQQMKTLSR
                     HSDTAKAFAYLLKQWKALNVYCSNGWVEIDNNIAENALRGVAVGRKNWLFAGSDSGGE
                     HAVVLYSLIGTCRLNNVEPEKWLRYVIEHLQDWPANRVRDLLP"
BASE COUNT          195 a          206 c          236 g          147 t
ORIGIN      
        1 gttcatgcgg atgatatccc cgttccggtc caggagccgg gcagtggtaa aacaagaact
       61 gcccggctgt gggtctatgt ccgtgatgac cgtaacgccg gttcacagat gcccccggcg
      121 gtctggttcg cgtacagccc ggaccggaaa ggtatccatc cacagaatca cctggctggt
      181 tacagcggtg tgcttcaggc agatgcttac ggtggttacc gggcgttata cgaatccggc
      241 agaataacgg aagccgcgtg tatggcccat gcccggagaa aaatccacga tgtgcatgca
      301 agagtgccaa ccgacatcac cacggaagcc ctgcagcgta tcggtgaact gtataccatc
      361 gaggcagaag tccggggatg ttcagcagaa cagcgtctgg cggcaagaaa agcaagagcc
      421 gcgccactga tgcagtcact gtatgaccgg atacagcaac agatgaaaac actgtcgcgt
      481 cactcggata cggcaaaagc gttcgcatac ctactgaaac agtggaaggc cctgaacgtg
      541 tactgcagta atggctgggt ggaaatcgac aacaacatcg cagagaacgc cttacgggga
      601 gtggccgtag gccggaaaaa ctggctcttc gcgggttccg acagcggtgg cgaacatgcg
      661 gtggtgttgt actcgctgat cggcacatgc cgtctgaaca atgtagagcc agaaaaatgg
      721 ctgcgttacg ttattgagca tctccaggac tggccggcaa accgggtacg cgatctgttg
      781 ccct
//
LOCUS       DADLFA010000372          783 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.374, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000372 DADLFA010000000
VERSION     DADLFA010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.374"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..360
                     /locus_tag="JQH65_004156"
     CDS             <1..360
                     /locus_tag="JQH65_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HAY6141345.1"
                     /translation="LPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKSN
                     DKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEGV
                     KPAVERVYTIKNTIDYY"
     gene            complement(363..>783)
                     /gene="speG"
                     /locus_tag="JQH65_004157"
     CDS             complement(363..>783)
                     /gene="speG"
                     /locus_tag="JQH65_004157"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HAY6141346.1"
                     /translation="TTSHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIIS
                     PEYQGKGLATRAAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHE
                     FFINGQYRNAIRMCIFQHQYLAEHKTPGQTLLKPTAQ"
BASE COUNT          228 a          176 c          174 g          205 t
ORIGIN      
        1 ttaccttctg ccggggtgag ttgggaaacc gatgataatg gcttcgtgat taaagccagc
       61 agcaaacaaa tgcagatgga atatcgctat gatgatcagg gttatccact gggtaaaacc
      121 acgaaaagta acgacaaaac attatctgtc agcgccacgc catcaacgga cccgatcaaa
      181 aaattagatt acacagcggt tactttactg aataatcaac gagttggtaa tgtaaaacag
      241 agctgtgaat atgacagtca cgccaatccg gtggactgtc agctgatcat tgttgatgaa
      301 ggagtaaaac ccgccgtcga acgggtttac accatcaaaa atacgatcga ttattattaa
      361 tgctattgtg cggtcggctt caggagagtc tgacccggtg ttttgtgctc tgccagatac
      421 tgatgctgga atatacacat gcgaatggca ttacgatatt gaccattaat aaaaaactca
      481 tgcatcaatt caccttcaac cgtaaagcca agcttgcggt aaatgtgaat cgctttttca
      541 ttctctttat cgacgatcag atacagctta tagagattga gaacggtgaa gccatagtcc
      601 attgctaatt tggcggcacg ggttgccaga cctttcccct gatactccgg ggagataatt
      661 atctgaaatt ctgcccggcg atgtacatgg ttaatttcca ccagctccac cagaccggct
      721 ttttcgccgt cacattccac cacaaagcgt cgttcgctct gatcgtgaat atgtgaagtg
      781 gtc
//
LOCUS       DADLFA010000373          772 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.375, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000373 DADLFA010000000
VERSION     DADLFA010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.375"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..>772
                     /locus_tag="JQH65_004158"
     CDS             <1..>772
                     /locus_tag="JQH65_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY6141347.1"
                     /translation="MPLLDKLRKLYGVGPVCSELHIAPSTYYHCQQQRHHPDKRSARA
                     QRDDWLKKEILRVYDGNHQVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGK
                     KVRTTISRKAVAAGDRVNRQFVAERPDQLWVADFTYVSTWQGFVYVAFIIDVFAGYIV
                     GWRVSSSMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTQRLKEAGLLASTG
                     STGDSYDNAMAESINGLYKAEVIHRKSWKNRAEVELATL"
BASE COUNT          184 a          184 c          235 g          169 t
ORIGIN      
        1 tgatgccact gctggataag ctgcgtaagc tgtacggggt cggaccggta tgcagtgaac
       61 tacatattgc cccgtcaacg tattaccact gtcagcaaca gcgacatcat cctgataaac
      121 gcagtgcccg tgcgcagcgc gatgactggc tgaagaaaga gatactgcgc gtatacgatg
      181 ggaatcatca ggtatacggt gtgcgtaaag tctggcgtca gttgttacgg gaaggtatca
      241 gagtggccag atgcactgtg gcacgtctca tggcggttat gggacttgcc ggtgttctcc
      301 ggggtaaaaa ggtccgtacg accatcagcc ggaaagccgt tgccgcaggc gaccgcgtaa
      361 accgtcagtt cgtggcagaa cgacctgacc agctgtgggt ggctgatttt acttacgtca
      421 gcacatggca gggcttcgtc tatgtggcgt tcattattga tgtgtttgcc ggatacatcg
      481 tggggtggcg ggtctcatcg tccatggaaa cgacattcgt gctggatgct ctggagcagg
      541 cgttatgggc ccgtcgaccg tccggcacgg tccatcacag tgataaaggt tctcagtatg
      601 tatcgctggc ctacacacag cggcttaagg aagccggatt actggcatca acaggaagta
      661 caggcgactc gtatgacaac gcgatggcgg agagcatcaa tggtctttac aaagcggagg
      721 taatacaccg taagagctgg aaaaaccgtg cagaagtgga actggccaca ct
//
LOCUS       DADLFA010000374          749 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.376, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000374 DADLFA010000000
VERSION     DADLFA010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.376"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..587
                     /locus_tag="JQH65_004159"
     CDS             <1..587
                     /locus_tag="JQH65_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY6141348.1"
                     /translation="NQLADKQTIKQTLFARQPAKIHKPYPTNSKIIMGDVLNHAALKQ
                     AMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVLSLGIYDEVPGKFGEWNNA
                     VIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYELTSRNEPFKGTIVSRKSV
                     AALITDIIDKPEKHIGENIGINQPVTDGDKPLFM"
BASE COUNT          246 a          147 c          151 g          205 t
ORIGIN      
        1 ttaatcaact ggcagataaa caaaccatta aacaaacttt atttgcccgt cagccagcaa
       61 aaatccacaa accctatcca actaatagca agattattat gggagatgta ctgaatcatg
      121 cagcactgaa gcaggccatg cagggacagg acgttgtgta tgccaacctt acgggggaag
      181 atttagatat tcaggcaaat tcagtgatcg ctgcgatgaa agcctgtgat gttaaacgtc
      241 tgatctttgt tctttccctc ggtatttatg atgaagttcc aggaaaattt ggcgaatgga
      301 ataatgcagt tattggtgag cccctaaaac cattccgcag ggctgcggat gcaattgagg
      361 cttctggact ggaatataca attcttcgcc ctgcctggct gactgatgaa gatattattg
      421 attatgagct tacctcacgc aatgaaccat tcaaaggtac gatcgtatca cgtaagagtg
      481 tcgcagcatt aatcacagat atcatcgata aacctgagaa acacattggt gagaatatag
      541 gtatcaatca acccgtgact gatggtgaca agccattatt catgtaaatc aattacatag
      601 ttaccatcgt aatgtactat gtgcagtacg atttattaaa cttattaagg tgaactgatc
      661 aatccgatgc gattaacatg tgtagaacca gtaagaataa aatacgccat gtatataaca
      721 ttacgactta catctaagtc agcataatt
//
LOCUS       DADLFA010000375          726 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.377, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000375 DADLFA010000000
VERSION     DADLFA010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.377"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            35..726
                     /locus_tag="JQH65_004160"
                     /pseudo
     CDS             35..726
                     /locus_tag="JQH65_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          170 a          181 c          200 g          175 t
ORIGIN      
        1 atttagtgta tgatggtgat tttaaggtgc ttgcgtggct tccatttcca tcagatgtcc
       61 ttcctgctcc gctactgaag gcgtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcattgcc gtaaaacatg gcaactacag ttcacttaca ccgcctctca
      181 gcccggtacg caccagaaaa tcattgatat ggccatgaat ggcgtcggat gtcgcgccag
      241 tgcacgcatt atgggcgttg gcctcaacac ggttttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acaaccgggc agtgatgtga ttgtctgcgc tgaaatggac
      361 gaacagtggg gctacgtcgg tgctaaatca cgtcagcgct ggctgtttta cgcgtatgac
      421 aggatacgga ggacggttgt ggcgcacgtc ttcggtgaac gcactctggc cacactggag
      481 cgtcttctga gcctgctgtc ggcctttgag gtcgtggtat ggatgacgga tggctggccg
      541 ctgtatgaat cacgcctgaa gggaaagctg cacgttatca gcaagcgtta cactcagcgc
      601 attgagcgac ataatctgaa tctgagacaa catctggcaa ggctgggacg gaagtcactg
      661 tcgttctcaa aatcggtgga gctgcatgac aaggtcatcg ggcattatct gaacataaaa
      721 cactat
//
LOCUS       DADLFA010000376          686 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.378, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000376 DADLFA010000000
VERSION     DADLFA010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.378"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            207..476
                     /locus_tag="JQH65_004161"
     CDS             207..476
                     /locus_tag="JQH65_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141349.1"
                     /translation="MAQVNMSVRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNSWFRDQVAAGRQQLECGDVLPHDMVESSAAAWRDEMSRKIADK"
     gene            473..600
                     /locus_tag="JQH65_004162"
                     /pseudo
     CDS             473..600
                     /locus_tag="JQH65_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553778.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT          174 a          157 c          198 g          157 t
ORIGIN      
        1 cacatcgcat aaaactgtca gcgccagtga taggacaggt gaaacctgtc aatacgtacc
       61 ggcttacgcc gggcttcggc ggttttactc ctctgccata tgcaacaacg gagtgccgcc
      121 ttacatgccg ctggcgcggc atatcctggt gacgatatct gaacagttat atactgtgta
      181 tatacgtggt aatgacgagg tggtaaatgg cacaggttaa tatgagtgta agaatcgacg
      241 cagaactgaa ggatgctttt atggccgctg caaaaagcat ggaccgtaat ggctctcagt
      301 taatccggga ttttatgcgc cagaccgttg aacggcagca caattcttgg ttccgtgacc
      361 aggttgcggc aggccgtcag caactcgaat gcggtgatgt gcttccccat gacatggtcg
      421 aatcctctgc tgctgcatgg cgggatgaaa tgagcaggaa gattgctgat aaatgatgga
      481 gatgttctgg accatgctgg ccatccagga caggaagcgc attcgtagta cattgcggag
      541 cagaacctga tggccgccat tgaaccggac gaacggattg gttattcagc cagcagcctg
      601 gcagggcagc cgtataaagg ccgcaacggt cgtgtggaag gaacatctgg accacacaag
      661 gtagcgtgta acgttatttt gtgtga
//
LOCUS       DADLFA010000377          685 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.379, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000377 DADLFA010000000
VERSION     DADLFA010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.379"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            201..389
                     /locus_tag="JQH65_004163"
     CDS             201..389
                     /locus_tag="JQH65_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HAY6141350.1"
                     /translation="MYKITATIEKEGGSPASWTRYSKTKLTKSECEKMLSGEKEAGVS
                     REQKVKLINFNCEKLQSS"
     gene            509..655
                     /locus_tag="JQH65_004164"
     CDS             509..655
                     /locus_tag="JQH65_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001377407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase XisR"
                     /protein_id="HAY6141351.1"
                     /translation="MVEYGLMLRTGLGARQIEAYRQNCWVEGFHFKRVSPLGKPDSKR
                     GIIW"
BASE COUNT          234 a          128 c          162 g          161 t
ORIGIN      
        1 cccgctcatg ggaaagaatc aaaggtatgg actggccgac aatcaaagct ggtcgtaatg
       61 cactatggat tgaactggaa ggcgttgaat atgcattccc ggatccacaa acacaaaacg
      121 aaacgcaaca agcaggatac gatgagaata ttccggtgta aaaccaagtg cattttggga
      181 aaatattaat ggaggtgttc atgtataaaa ttacagccac tattgaaaag gaaggtggtt
      241 ctcctgccag ctggacaaga tactcaaaga caaaactaac gaaatcagaa tgcgaaaaaa
      301 tgctctcagg ggaaaaagaa gctggcgttt ccagagagca gaaagtaaaa ctgataaatt
      361 ttaattgcga gaaacttcag tcctcgtgaa ttgcattgta ttcaaattaa aacttcatag
      421 ctgattatta ataatcaaca tcgggcgtca atttcagcct aacattggcg cctgccagag
      481 gtgatgcgat ggctttaatg aagagtggat ggttgaatac ggcctgatgc ttcgcactgg
      541 tctgggtgcc agacaaattg aagcataccg ccagaactgt tgggtggaag gcttccactt
      601 caaacgagta tctcctttag ggaagccaga cagtaagcga gggattatct ggtaaaacta
      661 tccgaagata aatcagttta tcaaa
//
LOCUS       DADLFA010000378          665 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.380, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000378 DADLFA010000000
VERSION     DADLFA010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.380"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            106..>665
                     /locus_tag="JQH65_004165"
     CDS             106..>665
                     /locus_tag="JQH65_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HAY6141352.1"
                     /translation="MPIIAPISRDERRLMHKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEHPVFYEDEVDIHL"
BASE COUNT          171 a          175 c          174 g          145 t
ORIGIN      
        1 cctaaatagc tgcgcggaat agtagatccc tttgagggaa cttagcccgg attgtgcgat
       61 ctgatcaatc gccaaatcaa aacaaatcac caaccggact gagcaatgcc gatcatagca
      121 ccaatttccc gtgacgaacg acgcctgatg cataaagcca tccataaaac acacgataaa
      181 aattatgccc gcagactgac tgccatgctg atgctgcacc ggggcgaccg tgtcagcgac
      241 gttgccagaa cgctctgctg cgcccgttcc tctgttggac gctggattaa ctggttcacg
      301 cagtcgggtg ttgagggact gaaatcatta cctgccgggc gtgcccgtcg ctggccgttt
      361 gagcatatct gcacactgtt acgtgagctg gtaaaacatt ctcccggcga ctttggctac
      421 cagcgttcac gctggagtac agaactgctg gcaataaaaa tcaatgagat aaccggttgc
      481 cagttaaatg ccggaaccgt tcgccgctgg ttgccgtctg cggggattgt gtggcgaagg
      541 gctgcgccaa ctctgcgtat ccgtgacccg cataaagatg aaaagatggc agcaatccat
      601 aaagcactgg acgaatgcag cgcagagcat ccggtctttt atgaagatga agtggatatc
      661 catct
//
LOCUS       DADLFA010000379          658 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.381, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000379 DADLFA010000000
VERSION     DADLFA010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.381"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(66..596)
                     /locus_tag="JQH65_004166"
     CDS             complement(66..596)
                     /locus_tag="JQH65_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY6141353.1"
                     /translation="MSITGVNIGRLFREGFSNYDPIGALNAMASQRTKEARGGELQIN
                     ELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATKMLFSHAQKTLAWDAFAFT
                     EVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVSLPDFSHYELYDKPEAV
                     QEALEKVIPFFNPHLG"
BASE COUNT          167 a          177 c          137 g          177 t
ORIGIN      
        1 atggcgggct atctgaccgc cagcaccaag aatcagtaca tttttcataa tatttctcat
       61 gaaacttatc ccagatgggg gttgaaaaaa ggaataactt tttccagcgc ctcctgaact
      121 gcttccggtt tgtcatacag ttcataatgt gagaaatcgg gtaatgaaac taactgacga
      181 tcctgcgaaa ccatcgcccg gccatagact tccagtccat cacgataagc accgaaggca
      241 ccgacttttt caccaacgac taccatcacc ggctgagtca gtaacacttc agtaaatgcg
      301 aaggcatccc aggccagcgt tttctgtgca tgtgagaaca acatttttgt cgcacctccc
      361 ggttgttgcc cgcgtggtgt tttatagtag tcagttgctt cataaacatc gcgttcggtc
      421 agtccatggg cttttgctgc atccaggctg gcgggaagta attcgttgat ctgtaattct
      481 ccaccacgag cctctttggt tcgttgcgac gccattgcat tcaatgcgcc aatcggatcg
      541 taattgctga aaccttcccg aaaaagacgc ccgatattaa cacccgtaat actcactact
      601 gctttaattc gtttttctgt cagcgcggca ttaatggcat agtcaccacc cccacaca
//
LOCUS       DADLFA010000380          656 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.382, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000380 DADLFA010000000
VERSION     DADLFA010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.382"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            215..574
                     /locus_tag="JQH65_004167"
     CDS             215..574
                     /locus_tag="JQH65_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HAY6141354.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMEFGERTVIK
                     NPEYFSTYMQEELKALV"
BASE COUNT          211 a          127 c          131 g          187 t
ORIGIN      
        1 aactatataa ataaacataa ccatgccgtt gtgttcgccc ttcgattagc acctctggtt
       61 ttctttctta gctgaacaat ctggttatag cgtgcggact gaccgttaat tttctccatc
      121 ttactggata acacaccatg agctgctacc tttaaaacac ctgaatgtca acaggttaac
      181 tcgtgccata tcgttataat ccctttgata ttagatgcaa attaaggtca tatatagcct
      241 tatcgataac atggttaatt ttaaggataa gaatatgcct gctgtaatag ataaagccct
      301 ggatttcatt ggtgccatgg atgtatcagc gccaacacca agttcgatga atgaaagcac
      361 agcgaaggga atctttaaat atttaaaaga actgggagta cccgcgagtg ccgctgatat
      421 tacggcgcga gctgaccagg aaggctggaa ccctgggttc acggaaaaaa tggttggatg
      481 ggcaaaaaaa atggagtttg gtgaacgcac tgtaattaaa aatcctgaat acttttcaac
      541 atatatgcag gaagaactaa aagcactggt ctgagtttaa tttatatcag cataaatggg
      601 tcaggacgct tttaatcaca tataaaaagc gtcctttttc tcacaatcaa ataatc
//
LOCUS       DADLFA010000381          632 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.383, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000381 DADLFA010000000
VERSION     DADLFA010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.383"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..>632)
                     /locus_tag="JQH65_004168"
     CDS             complement(<1..>632)
                     /locus_tag="JQH65_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY6141355.1"
                     /translation="VVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHIDAASGG
                     FLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYL
                     GGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKLGPYE
                     FICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEA"
BASE COUNT          145 a          182 c          183 g          122 t
ORIGIN      
        1 gcttcaccgc cgagagtgaa ggccggaacc tgccagccgc gcagacgcag acgttcagag
       61 aggtcgtaca gggtgtatcc cggatcttca ccatctttca gtttgaagca aaccgccggg
      121 atgccttcgt ccgggcgacc cgtacagatg aactcatacg gccccagttt ggcgatttca
      181 tccgccagat aagcggcaac ctggtaagag gcgttctgta ctttggtata gccttcacga
      241 ccgaggcgca ggaattcata gtactgtgca attacctgac ccgccgggcg ggagaagttg
      301 atggcaaaag taccaatttg accacccagg tagtcaacgt tgaacaccag ttcctgcggc
      361 agcgcttctt cgtcacgcca gataacccag ccgcagccca gcggagccag accgaattta
      421 tggcctgaag cactgatcga tttcacacgc ggcaggcgga agtcccagac gatatccggg
      481 gcgacgaacg gtgccaggaa gccaccgctg gcagcgtcga tgtgcatgtc gatgtcgata
      541 ccggtgtcgg cctggaattt atccagcgca tcgtgcagcg gttgtgggaa ctcatagtta
      601 ccggtgtagg tcacgccgaa agtcggcacc ac
//
LOCUS       DADLFA010000382          632 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.384, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000382 DADLFA010000000
VERSION     DADLFA010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.384"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
BASE COUNT          210 a          119 c          103 g          200 t
ORIGIN      
        1 tcccattcac aaacaaacat taattattat caacacattc acattaatta taaatcatct
       61 cagaccttcc tgataaaaag atattaaagt gaaacatttt gtttttcaca ttttagttca
      121 tcacttttag gcgtaaataa aggtaaaaaa aacaacaata ctatttctct ttcacaaaag
      181 aattaacaaa aggtttttta aggatgagtg atattattta cttgaccata accggagaac
      241 aacaaggatg tatttcctct ctttgtggaa cgagcgcatc aataggtaat cgctggcaga
      301 ttggccatga agatgaaatc ttcgcgttct cgctctctaa tagtattacg aatgcaggca
      361 aagggtcaca attgcatggc cttagtttct gtaaattctt attaacgcta ttaacaataa
      421 cgagcagttg ttcatggcgt ttgacttcta tcggatcaat agatttggtc gatgggagaa
      481 atattacaac attcaactcc gcggcgcgct cttatcagca atcaatcacc tgtttactga
      541 gaacaatctg gatacagaag ccatcactgt cagttatgaa tatatactct ccaggcatct
      601 gattgctaat actgaattta gttatttggc at
//
LOCUS       DADLFA010000383          619 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.385, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000383 DADLFA010000000
VERSION     DADLFA010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.385"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(165..>619)
                     /locus_tag="JQH65_004169"
     CDS             complement(165..>619)
                     /locus_tag="JQH65_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HAY6141356.1"
                     /translation="DVAPTGQQIWQELQSGKWGEIAPFTVTPEMLEAAREARRQEIEA
                     WRAEQEAKPFTFEWNGRIWNAGPDSLGRLSPVVMLAKSVTAQTHMAWSDADNQQVKLS
                     MPELEELAAAMVQAQVDRNDEIYRRQREMKEELSGLDDLASIRAFDVE"
BASE COUNT          117 a          206 c          145 g          151 t
ORIGIN      
        1 cacagaccgg actgactccg gaaaaacaga ggcccgcccc cgggcctccc cggattcatc
       61 cgtttccctg ttcagcctga cagcacgccc ccgcggccgg atgacagact ccgcttcggt
      121 aagcaaagcg gtcttctgtg attccgccag ttgcggctta ttcattactc aacgtcaaac
      181 gcccgaattg aagccaaatc atccagaccg ctcagctcct ctttcatttc acgctgacgg
      241 cgataaatct catcgttgcg atcgacctgc gcctgcacca ttgctgccgc cagttcttcc
      301 agttccggca tcgacagttt cacctgctga ttatcggcat cgctccacgc catatgtgtt
      361 tgtgctgtga cagattttgc cagcatgact accggggaca ggcggcccag tgagtcggga
      421 ccagcattcc agatacgacc gttccattca aacgtgaacg gcttcgcctc ctgttctgcg
      481 cgccatgctt caatttcctg acgtctggcc tctctggccg cttccagcat ttctggtgtc
      541 acagtgaatg gggctatctc accccatttg ccgctttgca gttcctgcca gatttgctga
      601 cccgtcggag cgacatcat
//
LOCUS       DADLFA010000384          606 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.386, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000384 DADLFA010000000
VERSION     DADLFA010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.386"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..>606
                     /locus_tag="JQH65_004170"
     CDS             <1..>606
                     /locus_tag="JQH65_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110-like element ISSso6 family transposase"
                     /protein_id="HAY6141357.1"
                     /translation="TVAYAINSEPVELMGKIGTSPTDIQNLCKRLRSKSSQVSIVYEA
                     GPCGYGLYRRLVKSGFDCMVCAPSLIPKKPGERVKTDRRDAIRLVRSLRAGDLSAVYV
                     PGIEDEAFRDLARAWASARDDLRHARQRLKSFLLVHGVHYVGRADWGPAHRRWLSKYS
                     FESPWRQLAFDEHRRTIEDRQAQCERLESALKEAVTEWRLYP"
BASE COUNT          139 a          150 c          165 g          152 t
ORIGIN      
        1 tactgtcgct tatgctatca attcagaacc tgttgaactg atgggtaaaa ttggcacatc
       61 acctactgat attcagaatc tttgtaaacg tctcaggtca aagtcatcgc aggtcagtat
      121 cgtttatgaa gcggggccct gtggttatgg actttatcgt cggctggtga aatccggttt
      181 tgactgcatg gtctgcgccc cctcacttat cccgaaaaaa ccgggggagc gggttaaaac
      241 cgatcgccgt gatgccatca gacttgtgcg ttcactgcgt gcaggagatc tttctgctgt
      301 ctacgtaccc ggcattgaag atgaggcatt ccgggatttg gcccgggcat gggcatctgc
      361 ccgcgatgat ttacggcatg caaggcaacg tctgaaatcg tttcttctgg ttcatggggt
      421 ccattatgtc gggcgagcag attggggccc tgcacaccga cgctggctca gcaaatactc
      481 attcgaaagt ccctggcgac aattagcttt tgacgaacac cgtcgtacaa tagaagaccg
      541 gcaggcacaa tgtgaacggc tggaatccgc cctgaaggag gccgttaccg aatggaggct
      601 gtatcc
//
LOCUS       DADLFA010000385          598 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.387, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000385 DADLFA010000000
VERSION     DADLFA010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.387"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            1..598
                     /locus_tag="JQH65_004171"
                     /pseudo
     CDS             1..598
                     /locus_tag="JQH65_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT          156 a          147 c          166 g          129 t
ORIGIN      
        1 attattttca acaggttgct ttcgctactt gatgcaaacg gctttattga ctggtctgcc
       61 accgcgctgg atggcagtaa tatccgggcg ctgaaatgtg ccgccggtgc tcaaaaaaac
      121 atcccgatat cgacggagat aatggcccgg gtcgctctcg cggcggtttt ggcaccaaaa
      181 tccatctggc aacagacgga agtggcctcc cgttaaatat cgtgctaagc cccggacaag
      241 ctcatgaaag ccagttcgcg caacgacttc tggacgggat tggcgttcag cgtcagaacg
      301 gcagcatgaa acgccgtgga catgcggtac tggctgataa agcgtattca gggcgcgcat
      361 tgcgcaacga gctgaaaaat aacggtataa aggcagtaat cccccgaaag tcaaatgaga
      421 aaatggcatc ggatggacgt gcacagcttg atcgtgatgc gtactgcaat cgtaatgtcg
      481 ttgaacggtg ctttgggcgg ctgaaagaat atcgccgcat cgccacgcgt tacgacaaaa
      541 cggcgagaaa ttacctggcg atggtgaaac tgggctgcat ccgactcttt tatcaacg
//
LOCUS       DADLFA010000386          590 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.388, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000386 DADLFA010000000
VERSION     DADLFA010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.388"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            244..>590
                     /locus_tag="JQH65_004172"
     CDS             244..>590
                     /locus_tag="JQH65_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY6141358.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNN"
BASE COUNT          170 a          110 c          122 g          188 t
ORIGIN      
        1 atcgccatac cctaaccaat tgcacttgtg aatttatttt tgcttaattt tgaattaaga
       61 aaatttaatt tgttcaaaag cccttaaagt gcacaatagg cgtcctaatg aacaatggtt
      121 gtaaattttg gtaacaaata tcattgctca ttaatatcta gcatctgctt ataacatttg
      181 tgtcatgaat agtcattctt caaaccagta gtctgactaa tggaatttta ttcaaggatt
      241 tttatgtcta aaaaattagg ttttgccctg tctggtctta tgctggcaat ggttgcaggt
      301 actgcttctg ctgatatgga tggtggtcag ttaaatatca gtggtctggt tgttgataac
      361 acctgtgaaa cccgcgttga cggcggtaat aaagatggtc tgatcctgct gcaaactgca
      421 actgttggtg aaatcgatgc tggcgtgctg aatgacaccg ttggcgctaa agccaaacct
      481 ttcagcatca ccgttgactg cagcaaagca aatccgaatc caggtagtac agctaaaatg
      541 accttcggtt ctgttttctt tggtaatagc aaaggaacgc tgaacaacga
//
LOCUS       DADLFA010000387          573 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.389, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000387 DADLFA010000000
VERSION     DADLFA010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.389"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..>573
                     /locus_tag="JQH65_004173"
     CDS             <1..>573
                     /locus_tag="JQH65_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MolR family transcriptional regulator"
                     /protein_id="HAY6141359.1"
                     /translation="RHINHPFALTLLIRVAGQTKRCHDRMTKAIAAFPHAAMAALTEL
                     LGQKEENSWRIMLMTMLISQPALAEQVLPWLSTPAVAVLKSYQQQLTQPSNHACADLL
                     PAVLVSPPWLSKKKKSVMPVLDLTPLPLESCCTLTETAEKEIHTRHRWHAHQIDIGQK
                     EDIQNYLARLGFNRWNNGQYMKASDAVVEL"
BASE COUNT          165 a          149 c          129 g          130 t
ORIGIN      
        1 tgcgtcatat caatcatccg ttcgcattga cactgcttat acgtgtagcc gggcaaacta
       61 aacgctgtca cgatcggatg acgaaagcca ttgctgcgtt cccacatgca gcaatggcgg
      121 cactgacgga acttcttggg caaaaagaag agaacagttg gcgcattatg ctaatgacaa
      181 tgcttatctc acaaccagca ctggcagaac aggtccttcc ctggctctcg acacccgcag
      241 ttgccgtact gaaatcatac cagcaacaac tgacacagcc ctcaaaccat gcctgcgccg
      301 atctactgcc tgccgtatta gtctcaccgc cctggctttc gaaaaagaaa aaatctgtaa
      361 tgccggtatt ggatttaacg ccattaccgt tggagtcatg ctgtacattg actgagacgg
      421 cagaaaaaga gattcataca aggcatcgct ggcacgcaca tcaaatagac atcggccaaa
      481 aagaggatat tcagaactac ctggcacgtc tgggtttcaa tcgctggaat aatggccagt
      541 atatgaaagc gtctgatgct gttgtagaac tct
//
LOCUS       DADLFA010000388          570 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.390, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000388 DADLFA010000000
VERSION     DADLFA010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.390"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..>570
                     /locus_tag="JQH65_004174"
     CDS             <1..>570
                     /locus_tag="JQH65_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HAY6141360.1"
                     /translation="DALQQSGGEDGGSVVFPPVLVQMLDRLESEILADRVSEESRRWL
                     ASCGLTVAQMKNQMDPVYTPARKIHLYHCDHRGLPLVLISTEGATEWCAEYDEWGNLL
                     NEENPHHLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNFYQYP
                     LSPVNSMDPLGLYEFKSKNIDDIGIFALA"
BASE COUNT          139 a          139 c          176 g          116 t
ORIGIN      
        1 cggatgccct tcagcagtcc ggcggcgaag acggtggcag tgtggtgttc ccgccggtgc
       61 tggtgcagat gctcgaccgg ctggaaagtg aaatcctggc tgaccgggtg agtgaggaaa
      121 gccgccgctg gctcgcatcg tgcggcctga ccgtggcgca gatgaaaaac cagatggacc
      181 cggtgtacac gccggcgcga aaaattcacc tgtaccactg cgaccatcgc ggcctgccgc
      241 tggtgcttat cagcacggaa ggggcaacag aatggtgcgc agaatacgat gagtggggca
      301 acctgctgaa tgaagagaac ccgcatcatc tgcagcagct cattcgcctg ccggggcagc
      361 agtatgatga ggagtccggc ctgtattaca accgccaccg ctattatgac ccgctgcagg
      421 ggcgatatat cactcaggat ccgattgggc tgaagggggg atggaatttt tatcagtatc
      481 cgctgagccc ggtgaatagc atggatccat taggattata tgaatttaaa tcaaaaaata
      541 tagatgatat tggaatattt gcattggcaa
//
LOCUS       DADLFA010000389          558 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.391, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000389 DADLFA010000000
VERSION     DADLFA010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.391"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            2..>558
                     /gene="fimZ"
                     /locus_tag="JQH65_004175"
     CDS             2..>558
                     /gene="fimZ"
                     /locus_tag="JQH65_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HAY6141361.1"
                     /translation="MIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDYLRTRP
                     VDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANGFVSKC
                     NDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRYLVSGL
                     SNKEIADKLLLSNKTVSAHKSNIYG"
BASE COUNT          180 a          102 c          106 g          170 t
ORIGIN      
        1 ggtgatcatt atggatactc atcctatcat cagaatgtct attgaagttc tgttgcaaaa
       61 aaacagtgaa ttgcagattg tcctgaaaac ggatgattac cgcataacca tcgattatct
      121 ccgaacccgt cctgttgatt taatcattat ggatatagac ttgcccggaa cagacggttt
      181 taccttcctg aaaaggatca aacaaatcca gagcacagtg aaagtgttat ttttatcatc
      241 gaaatcagaa tgcttttatg ctggcagagc gatacaagct ggtgctaacg gttttgtcag
      301 taaatgcaat gatcagaatg atatttttca tgccgttcag atgatcctct ccggatacac
      361 gttttttccc agcgaaacgc ttaactatat aaaaagcaat aaatgtagta cgaatagttc
      421 aacggtcact gtgctatcta atcgtgaagt gaccatatta cgttatctgg ttagcggatt
      481 atctaataaa gaaattgccg ataagttatt gcttagcaat aaaacagtta gtgcgcataa
      541 atctaatatt tatggcaa
//
LOCUS       DADLFA010000390          544 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.392, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000390 DADLFA010000000
VERSION     DADLFA010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.392"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(50..>544)
                     /locus_tag="JQH65_004176"
     CDS             complement(50..>544)
                     /locus_tag="JQH65_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HAY6141362.1"
                     /translation="SVDYNIEGFGFVGAYSKSDRTNEQAGDGYGDNAEVWSLAAKYDA
                     NNIYAAMMYGETRNMTVLANDHFANKTQNFEAVVQYQFDFGLRPSLGYVYSKGKDLYA
                     RDGHKGVDADRVNYIEVGTWYYFNKNMNVYTAYKFNLLDKDDAAITDAATDDQFAVGI
                     IYQF"
BASE COUNT          150 a          119 c          111 g          164 t
ORIGIN      
        1 tcaggctagt gttatgtttt acagtaacac gccgcggcgc gttggctgat tagaactggt
       61 agatgatacc aactgcaaac tggtcatcag ttgcggcatc ggtaatcgct gcatcgtctt
      121 tatccagcag gttaaatttg tatgctgtgt agacgttcat gttcttattg aagtagtacc
      181 aggtaccaac ttcgatataa tttacgcggt cagcatcaac acctttatgt ccatcacgag
      241 cataaagatc tttgcctttg gaatatacgt agcctaaaga cggacgtaaa ccgaagtcga
      301 actgatactg tacaacagct tcaaagtttt gggttttatt tgcaaaatga tcattagcca
      361 aaacggtcat gttgcgggtt tcaccgtaca tcattgctgc atagatatta tttgcatcat
      421 acttggctgc taatgaccac acttcagcgt tatcaccgta gccgtcacca gcttgttcat
      481 tagtacgatc agatttgcta tatgcaccta caaaaccaaa accttcgatg ttgtagtcta
      541 caga
//
LOCUS       DADLFA010000391          542 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.393, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000391 DADLFA010000000
VERSION     DADLFA010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.393"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            37..>542
                     /locus_tag="JQH65_004177"
     CDS             37..>542
                     /locus_tag="JQH65_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAY6141363.1"
                     /translation="MNQKYLIRIAELECQLRQKDQQLSLVEETEAFLRSALARAEEKI
                     EEDEREIEHLRAQIEKLRRMLFGTRSEKLRREVEQAEALLKQREQDSDRYSGREDDPQ
                     VPRQLRQSRHRRPLPEHLPREINRLEPEESCCPECGGELDYLGEVSAEQLELVSSALK
                     VIRTERVK"
BASE COUNT          155 a          131 c          158 g           98 t
ORIGIN      
        1 ttgtaaaaat gtcatggccg gattataaaa acggccatga atcaaaaata cctcattcgc
       61 attgcagaac tggaatgcca gctccgtcag aaagaccagc aactgagtct ggttgaagag
      121 acggaggcct tcctgcgctc tgcactggcc cgcgccgaag aaaagatcga agaagatgaa
      181 cgggaaatag aacatctgcg ggctcagata gaaaaactgc gccggatgct gttcggtacc
      241 cgttctgaaa aactgcgtcg tgaagttgaa caggctgagg ccctgctgaa acaacgcgaa
      301 caggacagtg atcgttacag tgggcgggaa gacgatccgc aggttccccg ccagttgcga
      361 cagtctcgtc atcgtcgccc gttaccggag catctgcccc gcgaaataaa tcgcctggag
      421 ccagaagaaa gctgttgccc ggagtgtggc ggtgagctgg attatctggg ggaagtcagc
      481 gcagaacaac tggaactggt gagcagcgct ctgaaagtga tccgcacaga acgggtaaaa
      541 aa
//
LOCUS       DADLFA010000392          527 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.394, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000392 DADLFA010000000
VERSION     DADLFA010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.394"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..325
                     /gene="dsbG"
                     /locus_tag="JQH65_004178"
     CDS             <1..325
                     /gene="dsbG"
                     /locus_tag="JQH65_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HAY6141364.1"
                     /translation="SGKVQLRTLLVGVIKPESPATAAAILASKDPAKTWQQYKASGGK
                     LKLNVPANVSTEQMKVLSDNEKLMDDLGANVTPAIYYMSKENTLQQAVGLPDQKTLNI
                     IMGNK"
BASE COUNT          191 a           98 c          114 g          124 t
ORIGIN      
        1 ttctggcaaa gtgcaattaa gaacattgtt ggttggggtt atcaagccag aaagcccggc
       61 gacagcagcg gcaattcttg cctccaaaga tcccgcaaaa acctggcaac aatataaagc
      121 ctctggtggc aagcttaagc taaacgtgcc tgcaaacgta agtacagagc aaatgaaagt
      181 gttaagtgac aatgagaaac tgatggacga tctgggggca aatgtcacgc cggctatcta
      241 ttacatgagt aaggaaaata cgctacaaca ggccgtgggg ttgcccgatc agaaaacgct
      301 taatatcatt atggggaata aataaaaggt aattcatgga tggttgaact atatctctgc
      361 ttttcaacca tcctaaaaag catgaattgc tcgctctgcc ctaagtggct gtgctatttt
      421 agaaaaactt aatcctgagt taagcaaaaa aacataatca ataaaaatat atacgcagaa
      481 aaacattgat taagtgaata tatcatggaa gaaaaatata accggag
//
LOCUS       DADLFA010000393          522 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.395, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000393 DADLFA010000000
VERSION     DADLFA010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.395"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..>522
                     /locus_tag="JQH65_004179"
     CDS             <1..>522
                     /locus_tag="JQH65_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAY6141365.1"
                     /translation="EAKTDARDAAIIAEAARTLPHALRTLKLADEQIAELSMLCGFDD
                     DLAAQTTQASNRIRGLLTQIHPALERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKL
                     AAQLCKLAPRLGKRLAADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVA
                     LEVEQRVLAHPLYP"
BASE COUNT          114 a          173 c          133 g          102 t
ORIGIN      
        1 tgaagctaaa actgatgctc gtgacgctgc catcattgcc gaagctgccc gtaccctgcc
       61 tcacgcgcta cgcacgctga aactggctga cgagcaaatc gccgaactct ccatgctctg
      121 cggcttcgat gatgatcttg ccgcacagac aacgcaggcc agcaaccgta tccgcggcct
      181 tctgacccag atacatccgg cactggagcg cgttctcggt ccgagacttg agcacccggc
      241 ggtactcgat cttctccagc gatatccctc accagaaaaa ctcgcttcgc tgggtgagaa
      301 gaagctggca gcccagctct gcaaacttgc gcctcgtctg ggtaaacgcc ttgcagcaga
      361 catagctcag gcactggccg aacaaaccgt cgtcgttccc ggcacgaatg ccgctgccgt
      421 agtactgcca cgtctggcac tccagctcat cacgctgcgt aagcaaagag acgaggtggc
      481 gcttgaggta gaacagcgag ttcttgctca ccctctttac cc
//
LOCUS       DADLFA010000394          515 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.396, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000394 DADLFA010000000
VERSION     DADLFA010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.396"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(1..104)
                     /locus_tag="JQH65_004180"
                     /pseudo
     CDS             complement(1..104)
                     /locus_tag="JQH65_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672075.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1472 domain-containing protein"
     gene            235..515
                     /locus_tag="JQH65_004181"
                     /pseudo
     CDS             235..515
                     /locus_tag="JQH65_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322674.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          132 a          136 c          126 g          121 t
ORIGIN      
        1 tccccgccag cccggctaag atggaggcac ggcacagccc gcacacgacg ctgaggctac
       61 agagctgaaa acccacaagg ccatgccccg catggccggg taaagcggag ccggcggcga
      121 cttcctttgt gctctgtccg ggaccggcag gaaaaaggcg gggataatga gatggtcacc
      181 cccaccctaa cagcagtacg ctgttaaggt gaatccctta acggagagac catcatacag
      241 aacatcacac ttattggtat cgatctcgga aagcattcct tccacattca ttgtcaagac
      301 aagtctggta aggctcttct gcgtaaaaaa tttacccagt actaaactga tgcagtttct
      361 ggcaacatgt ccgccctcag ttgttgtaat ggaagcctgt gcgggtgcac actttttggc
      421 tcgccgtatc tctgaatttt ggacatgaaa ctaagctgat ttctccacag ttcgttcgcc
      481 cgtttgtcaa aagtaataag aatgattttg ttgat
//
LOCUS       DADLFA010000395          514 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.397, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000395 DADLFA010000000
VERSION     DADLFA010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.397"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..279
                     /locus_tag="JQH65_004182"
     CDS             <1..279
                     /locus_tag="JQH65_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAY6141366.1"
                     /translation="SLNVYCRNGQVEIDNNIGENALRSVAVGRKNYLFFGSDNGGESA
                     AIIYSLLVTCKLNGVEPEDWLREVIVKLNDWPSNRVHELLPWNFSAVK"
BASE COUNT          125 a          115 c          139 g          135 t
ORIGIN      
        1 agcctgaacg tgtactgtcg taatggccag gtggaaatcg acaacaacat cggtgaaaac
       61 gcgctgcgat cggtggcggt tggaagaaaa aattatctct tcttcggttc tgacaacggt
      121 ggagaaagcg cggcgattat ctacagtctg ctggtcacct gcaaactgaa cggagtggag
      181 ccggaggact ggttgcgcga agtgatcgtg aagctcaatg actggccgtc gaaccgagtg
      241 catgaactgc tgccctggaa cttctcagct gtaaaataat ccttccgcta cgtacttctc
      301 tgggcgctta ccactcaacg gcatcgtcaa aattatcaaa gcgcagaaca tagatttatc
      361 atgtgtttta tggatggctt tctccatcag gcatcgttcg tcacgggaaa ttggtgctat
      421 gatcggcatt gatcagtccg gttggtgatt tgttttgatt tggcgattga tcagatcgca
      481 caatccgggc tgagttccct ctaagtgatc tagg
//
LOCUS       DADLFA010000396          477 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.398, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000396 DADLFA010000000
VERSION     DADLFA010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.398"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(231..>477)
                     /gene="dgcZ"
                     /locus_tag="JQH65_004183"
     CDS             complement(231..>477)
                     /gene="dgcZ"
                     /locus_tag="JQH65_004183"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HAY6141367.1"
                     /translation="AANDEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPE
                     EPLDVVIGRADRAMYEGKQTGRNRCMFIDEQNVINRV"
BASE COUNT          123 a          115 c          100 g          139 t
ORIGIN      
        1 tgggatgact aatgaacgga gataatccct cacctaaccg gccccttgtt acagttgtgt
       61 acaaggggcc tgatttttat gacggcgaaa aaaaaccgcc agtaaaccgg cggtgaatgc
      121 ttgcatggat agatttgtgt tttgctttta cgctaacagg cattttcctg cactgataac
      181 gaatcgttga cacagtagca tcagttttct caatgaatgt taaacggagc ttaaactcgg
      241 ttaatcacat tttgttcgtc aataaacata cagcgatttc ttcccgtttg cttaccctca
      301 tacattgccc ggtctgctct tccaatgacc acatccagag gctcttcagg aaatgcgcga
      361 ctgacacctg ctgtcacggt aatgttgata tgcccttcag aatgtgtgat ggcatggtta
      421 tcgactaact ggcaaattct gacacctgca cgacatgctt cttcatcatt agccgct
//
LOCUS       DADLFA010000397          476 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.399, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000397 DADLFA010000000
VERSION     DADLFA010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.399"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..334)
                     /locus_tag="JQH65_004184"
     CDS             complement(<1..334)
                     /locus_tag="JQH65_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAY6141368.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPES"
BASE COUNT          147 a          103 c          111 g          115 t
ORIGIN      
        1 tactttccgg taaaaataaa aacggtaaat agagcgcaat atcggaaata acatcgccag
       61 tttcattcaa aatcgccccc agacgtgttt gctgattgca ctcacgcgcc aacatgccat
      121 ccagcgcatt gagcgccata cggataaaaa gcacgatggg caatagcaaa aagaggatgg
      181 gttgtgccac caacaccaac agcaatccgg taaaaagaga aagcgccagt gccgtaagag
      241 tgatgtgatt cgctgtaacg tggtgcttat aaagccaaaa catcgtcggc cttaacagcg
      301 actgaaagag cggttttatc tggtagagcg tcatttatca ttccgtgata ttcagtttgc
      361 tctatggtgc atcaattgtg atggcggaga tatcacaacg cggagcatca acgtaaattc
      421 ggaatatatc aagatgtggt ggatggtttt cctacacatc tgtttgatta ccgact
//
LOCUS       DADLFA010000398          468 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.400, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000398 DADLFA010000000
VERSION     DADLFA010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.400"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            109..468
                     /locus_tag="JQH65_004185"
                     /pseudo
     CDS             109..468
                     /locus_tag="JQH65_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          121 a          115 c          110 g          122 t
ORIGIN      
        1 tttttaacgt aaggtgagag gatttttaag tgtatccata gattaaaaat gacaatgcct
       61 ccaattagtt gaattagggg ttataatacc cgcttttaag gttctttcat ggtcacagtt
      121 aactttcatt gcccccgttg ccagtccgtt caggtttacc gtcacgggca gaacccaaaa
      181 ggacatgatc gattccgttg ccgtgactgt caccgggtgt ttcagctcac ttactgttac
      241 gaggcccgta aaccgggagt taaaaaccaa atcactgaaa tggcttcaac ggtgccggag
      301 ttcgcgatac tgcccgtacg ctgaaaatcg gcatcaatac cgtcatacgg acattaaaaa
      361 gctcccgccc cggcggataa catctgcgcc agtcgctcac gctgatgttg cccttatctg
      421 tgaacttgat gggcagtgga gcttcgttgg cagcaaggcg caacaaca
//
LOCUS       DADLFA010000399          438 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.401, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000399 DADLFA010000000
VERSION     DADLFA010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.401"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(1..108)
                     /locus_tag="JQH65_004186"
                     /pseudo
     CDS             complement(1..108)
                     /locus_tag="JQH65_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
BASE COUNT          128 a          120 c           90 g          100 t
ORIGIN      
        1 agagacaccc acttcccatt tctgataccg tagtggactc attcccgcaa gctgcctgaa
       61 gtgttgatgg aaagagggcg tgctcatttg cacgcggttt gccagcttga gtcttcaacg
      121 ctgggaagat gcggcaaacg gcaaatctgc ataccagcca gcctatatcc gtcatgcaca
      181 ggccaattat gaaagaggag aggcttaagt tctggaaccc tgaacgaata acgactacca
      241 gaaaagcact tcgcatcact cagatatgat ttaacataat atacattatg cgcaccaaca
      301 taaaccaagg gagaacccag tcgttggcgg tcatggttgc tcattaacaa cgaccaaacc
      361 ccatatctca cttactaccg tattctcggc ttaaccgtcc atgcaacctc aacacattgg
      421 tttcaactgc cgtcacca
//
LOCUS       DADLFA010000400          432 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.402, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000400 DADLFA010000000
VERSION     DADLFA010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.402"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..>432
                     /locus_tag="JQH65_004187"
     CDS             <1..>432
                     /locus_tag="JQH65_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY6141369.1"
                     /translation="AEQAKNDIDAALTGTLKMANHLSEIAAAGEKAQQKSRDNLGLKS
                     AATMEAQSDIYDRTKGRLAIPGAFGFGCAFLPEDVIRFDTKSDFLAWVRNALPGEYSV
                     AGPYDIIIPDTRFEGVLSIRWTDARPETTEPRYRAKSLTFYG"
BASE COUNT          113 a          112 c          119 g           88 t
ORIGIN      
        1 gcagaacagg ccaaaaatga cattgatgct gcgctgaccg gcactctgaa aatggctaac
       61 catctgtcag aaatcgcagc agcaggcgaa aaggcacaac agaagtcccg ggataatctg
      121 gggctgaaaa gtgcggccac gatggaagca cagagcgaca tttacgaccg gacaaaaggc
      181 cgtctggcga tacccggcgc attcggcttt gggtgtgctt ttctgcctga agatgttatc
      241 cgttttgaca ctaagagtga tttcctggcc tgggtaagga atgcgctgcc aggtgaatat
      301 tccgttgctg gcccctacga catcatcata cccgacacac ggtttgaagg ggtgctcagc
      361 atccggtgga ctgatgcacg ccctgagaca acagaaccgc ggtacagagc caaatccctt
      421 actttttacg gc
//
LOCUS       DADLFA010000401          431 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.403, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000401 DADLFA010000000
VERSION     DADLFA010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.403"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..>431
                     /locus_tag="JQH65_004188"
     CDS             <1..>431
                     /locus_tag="JQH65_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY6141370.1"
                     /translation="FPGGEMADLYSHRWEIELGYREIKQTMQLSRLTLRSKKPELVEQ
                     ELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPEL
                     MRDLASMGQLVKLPTRRGRAFPRVVKERPWKYPTAPKKSQS"
BASE COUNT          128 a           84 c          134 g           85 t
ORIGIN      
        1 cttccccgga ggagaaatgg cggatctgta cagtcatcgc tgggaaattg aactgggata
       61 cagggagata aaacagacga tgcaactgag caggctgacg ctgagaagta aaaagccgga
      121 gcttgtggag caagagctgt ggggtgtctt actggcttat aatctggtga gatatcagat
      181 gattaaaatg gcggaacatc tgaaaggtta ctggccgaat caactgagtt tctcagaatc
      241 atgcggaatg gtgatgagaa tgctgatgac attgcagggc gcttcaccgg gacgtatacc
      301 ggagctgatg cgcgatcttg caagtatggg acaacttgtg aaattaccga cgagaagggg
      361 aagggccttc ccgagagtgg taaaggagag gccctggaaa taccccacag ccccgaaaaa
      421 gagccagtca g
//
LOCUS       DADLFA010000402          416 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.404, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000402 DADLFA010000000
VERSION     DADLFA010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.404"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..258
                     /locus_tag="JQH65_004189"
     CDS             <1..258
                     /locus_tag="JQH65_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141371.1"
                     /translation="LNYGNFDARYKHRTEGVQDVRYGYEMYYNPGSNTVSWTFRSPSG
                     HGLSGISISDTGRNSADNVNGVYYRPLQKLINGIWYNVASI"
     gene            318..>416
                     /locus_tag="JQH65_004190"
     CDS             318..>416
                     /locus_tag="JQH65_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY6141372.1"
                     /translation="MTKQYDVTWLFSEDGKNWYEEQKNFASDTIKMV"
BASE COUNT          132 a           84 c           99 g          101 t
ORIGIN      
        1 ttaaattacg gaaacttcga tgcccgctat aaacatcgaa ctgagggggt acaggatgtg
       61 cggtatggtt atgaaatgta ttacaaccct ggcagcaaca ccgtttcatg gacgtttcgc
      121 tcaccttcgg gacacgggtt gtcagggata tcgatatcgg ataccggccg taactcagcg
      181 gataacgtca acggtgtgta ttaccgaccg ctgcaaaaac tgattaatgg catctggtat
      241 aacgtagcga gtatttaaca atgttgcatt taaaaaatat tacggcaggt aatccgaaaa
      301 ccgcggaaca atatcagctg acaaaacagt atgatgtcac ctggcttttt tcggaagacg
      361 gcaaaaactg gtatgaagaa cagaagaact ttgccagtga caccataaaa atggtt
//
LOCUS       DADLFA010000403          414 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.405, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000403 DADLFA010000000
VERSION     DADLFA010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.405"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            113..>414
                     /gene="tnpB"
                     /locus_tag="JQH65_004191"
     CDS             113..>414
                     /gene="tnpB"
                     /locus_tag="JQH65_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAY6141373.1"
                     /translation="MISLPSGTRIWLVAGITDMRKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDMIKILWADADGLCLFTRRLEEGQFIWPAVRDGKVSITRSQLAMLLDKL"
BASE COUNT           86 a          110 c          118 g          100 t
ORIGIN      
        1 cagttagtgc cagctcctgc aaggtggagt tccgtcacgg taacatgacg ctggaaaatc
       61 cttcaccaga gctgctcaca gtgttgatcc gtgaactgac cgggagggga cgatgatctc
      121 actcccgtca ggcacccgca tctggctcgt tgctgggata accgatatgc gtaagtcttt
      181 caacgggctg ggtgaacagg tacagcatgt gctgaatgat aatcccttct ccggtcacct
      241 gttcatcttc cgtggccgac ggggtgacat gattaaaatc ctgtgggctg atgctgatgg
      301 tctgtgcctg ttcaccagac gcctggagga aggccagttt atctggcctg ctgtgcgtga
      361 cggcaaggta tccattaccc gctcgcaact ggcaatgctc ctcgataagc tgga
//
LOCUS       DADLFA010000404          408 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.406, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000404 DADLFA010000000
VERSION     DADLFA010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.406"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..>408
                     /gene="bluF"
                     /locus_tag="JQH65_004192"
     CDS             <1..>408
                     /gene="bluF"
                     /locus_tag="JQH65_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HAY6141374.1"
                     /translation="FVLATEQSTYFEIPAEDSWLFIADGSDKELDSCALSPTINDHFA
                     FHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTADLKSKALAFTMAHALE
                     LGDKMISINLLPMTLVNEPDAVSFLLNEIKANAL"
BASE COUNT          114 a          102 c           79 g          113 t
ORIGIN      
        1 ttttgtcctt gcaaccgaac aatcaaccta tttcgagatc cctgccgaag actcctggct
       61 ttttatcgct gacggatctg ataaagaact tgattcctgt gccctttcac caactataaa
      121 cgaccacttt gcctttcatc ctattgtcga tcccttatcg cggcggataa tcgcttttga
      181 agccattgtg caaaaaaatg aagatagccc atcagccata gcggttgggc agcgtaaaga
      241 cggggaaatc tacacagcgg atctcaaaag taaggcgctt gcattcacga tggcacacgc
      301 acttgagctc ggtgataaaa tgatttcaat caatctatta cctatgaccc tggttaacga
      361 acctgacgca gtctcttttt tacttaatga aataaaggcc aatgctct
//
LOCUS       DADLFA010000405          405 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.407, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000405 DADLFA010000000
VERSION     DADLFA010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.407"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..331
                     /locus_tag="JQH65_004193"
     CDS             <1..331
                     /locus_tag="JQH65_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HAY6141375.1"
                     /translation="CTGEGDDGYFKTGRTFADEHYLKSRVTVVDVSAPDVLALLKELE
                     VKDKRIAELTDALTQMINAHKTTIRFGHERITECGGDCDSPEKMISENPDIRMAEAVL
                     RAGIKTE"
BASE COUNT          131 a           75 c           99 g          100 t
ORIGIN      
        1 ctgcacaggt gagggggatg atggatattt taaaactggt cgaacttttg cggatgagca
       61 ttacctgaaa tcgcgcgtaa ccgtcgttga tgttagcgct cctgacgtac tggctttact
      121 gaaagagctg gaagttaaag acaaacgcat tgcagaattg acagatgcgc ttacacaaat
      181 gattaatgcg cacaaaacca caattcgttt tggtcatgaa cgcataactg aatgtggtgg
      241 tgattgcgac tcgccggaaa agatgatttc agaaaatccg gatatcagaa tggcagaggc
      301 tgttttgaga gcaggaataa aaactgaata attaaattta gcacagcaaa taaaatttaa
      361 tccttaaccg gagggatttc tgcaccctca gaacatcagg aggcc
//
LOCUS       DADLFA010000406          396 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.408, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000406 DADLFA010000000
VERSION     DADLFA010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.408"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..191)
                     /locus_tag="JQH65_004194"
     CDS             complement(<1..191)
                     /locus_tag="JQH65_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 domain-containing protein"
                     /protein_id="HAY6141376.1"
                     /translation="MGKEYKTLINKALERFYFRLSASGAHAERAARDSLTRAIRSLYD
                     VAFYADDLDALNELSELICA"
     gene            complement(203..>396)
                     /locus_tag="JQH65_004195"
     CDS             complement(203..>396)
                     /locus_tag="JQH65_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141377.1"
                     /translation="AGKNGADEATYVYLNGTRLLVELPYQTVQEIISEAEKARQVNGD
                     EPYIEIICMDSEAEIQKAD"
BASE COUNT           98 a           98 c           85 g          115 t
ORIGIN      
        1 gcacagatca gctcggaaag ttcgttaagt gcatccagat catcagcgta aaaagccacg
       61 tcatacagac ttcggattgc tctggtcaat gagtcacggg ccgcacgttc agcatgagcg
      121 cctgatgcac ttaagcgaaa ataaaaacgc tcaagtgctt tgttaatgag agttttatat
      181 tctttgccca tcacaacgcc ctttaatctg ctttctgtat ttcagcttct gaatccatac
      241 aaataatttc gatatagggt tcatcgccat taacctggcg tgccttttca gcttcgctaa
      301 tgatttcttg tacggtctgg tacggaagtt ccacaagcag tcgcgtgccg ttcagataaa
      361 cgtaagtggc ttcgtcggct ccgtttttac ccgccg
//
LOCUS       DADLFA010000407          387 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.409, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000407 DADLFA010000000
VERSION     DADLFA010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.409"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            195..377
                     /locus_tag="JQH65_004196"
     CDS             195..377
                     /locus_tag="JQH65_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAY6141378.1"
                     /translation="MVDYVNVPRTIATVISSGKASKAELDSVLGVQDLWDLLEIIHVD
                     AHNEQVIQENRNGAGT"
BASE COUNT           91 a           88 c          102 g          106 t
ORIGIN      
        1 atggactgtg tgcagtttgt tcccgatccg aaaaaacgca gcatccggag accgcttgtt
       61 gaagaggata ttgaagaaat cattacccgt cttaatctgc gtgcggaggt ctttaagctg
      121 catgtggatt ttttcgctac cgtcgtcagc tagatatccc cccgcgttat ctcgaccccg
      181 acagaccgtt cggggtggtg gattacgtta atgttccccg taccattgcg accgttatct
      241 cctccggtaa ggcttcaaaa gctgaactgg attctgtcct tggtgtgcag gacttatggg
      301 atctgctgga aataattcat gtggatgccc ataacgaaca ggtgatacag gagaacagaa
      361 atggcgcagg tacttgatga gcttgtt
//
LOCUS       DADLFA010000408          378 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.410, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000408 DADLFA010000000
VERSION     DADLFA010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.410"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            46..>378
                     /locus_tag="JQH65_004197"
     CDS             46..>378
                     /locus_tag="JQH65_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141379.1"
                     /translation="MLLFLYINKDNIKVIYSLKINQTTPGILVSCDSNNNFACQTTMT
                     EDVIQRITTFFHTSPDVKNREIKLEWSGDKRDLPTAEAEISRVQASIIKWYASEYHNG
                     RQVLDEIQI"
BASE COUNT          138 a           69 c           76 g           95 t
ORIGIN      
        1 aaaaaaagaa caaatggtag tgtccgctat tgccagtaca cctcagtgct gttgtttctg
       61 tatatcaata aagacaatat aaaagtgatt tacagcctaa aaataaacca gacaacgcca
      121 ggtatacttg ttagctgtga tagcaataat aattttgcct gtcagaccac aatgactgaa
      181 gatgttatac agcgaattac tacatttttt cacaccagcc cagatgtcaa gaacagagaa
      241 ataaagctgg aatggtcagg agataagaga gatttaccaa ctgctgaagc ggaaatatct
      301 cgcgtgcagg cgtctattat caaatggtat gcgtcagaat accataatgg caggcaagtt
      361 ctcgatgaga tacaaata
//
LOCUS       DADLFA010000409          369 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.411, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000409 DADLFA010000000
VERSION     DADLFA010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.411"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..>369
                     /locus_tag="JQH65_004198"
     CDS             <1..>369
                     /locus_tag="JQH65_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY6141380.1"
                     /translation="VWVGKDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFVF
                     DYRLKAEDERDALLAQVSARTGEWEEDLLLGLISDEDREKLKAYRIYAKSLQAMDFSV
                     ITDKTSYNAIEWPASPEISS"
BASE COUNT           93 a           91 c          101 g           84 t
ORIGIN      
        1 ggtgtgggtc ggtaaggatg tgacaggcat tgaaccccgt aacgccagtg ttattgaagt
       61 tcctgatatt accgccaacc gccgcattac cgcgccgggt tactggtttt accgcaatga
      121 tgaatttgtc tttgactaca gactcaaagc ggaagatgag cgtgatgccc ttctggctca
      181 ggtcagtgcc cggacagggg aatgggaaga agacctgctg ctggggctaa tcagcgacga
      241 agaccgggaa aaactgaaag cgtaccgcat ttacgcgaaa tcgctgcagg cgatggattt
      301 cagtgtcatc actgataaaa cctcatacaa cgccattgaa tggcccgcct ctccggaaat
      361 ttcttcctg
//
LOCUS       DADLFA010000410          366 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.412, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000410 DADLFA010000000
VERSION     DADLFA010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.412"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            17..>366
                     /gene="leuE"
                     /locus_tag="JQH65_004199"
     CDS             17..>366
                     /gene="leuE"
                     /locus_tag="JQH65_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HAY6141381.1"
                     /translation="MVLVPGPNTLFVLKNSVSSGMKGGYLAACGVFIGDAVLMFLAWA
                     GVATLIKTTPILFNIVRYLGAFYLLYLESKILYATLKGKNNEAKSDEPQYGAIFKRAL
                     ILSLTNPKAILFYVS"
BASE COUNT           87 a           72 c           92 g          115 t
ORIGIN      
        1 ggttggggcc atttttattg tgttggtgcc tgggccaaat accctgtttg tactcaaaaa
       61 tagcgtcagt agcggtatga aaggcggtta tcttgcggcc tgcggtgtat ttattggcga
      121 tgcagtattg atgtttctgg catgggctgg agtggcgaca ttaattaaga ccaccccgat
      181 attattcaac attgtacgtt atcttggtgc gttttatttg ctctatctgg agagtaaaat
      241 tctctacgcg accctgaagg gtaaaaataa cgaggccaaa tccgatgagc cccaatacgg
      301 ggccattttt aaacgcgcgt taattttgag cctgactaat ccgaaagcca ttttgttcta
      361 tgtgtc
//
LOCUS       DADLFA010000411          364 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.413, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000411 DADLFA010000000
VERSION     DADLFA010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.413"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..>364
                     /locus_tag="JQH65_004200"
     CDS             <1..>364
                     /locus_tag="JQH65_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY6141382.1"
                     /translation="IKVAHIRTRETYGTRRLQTELAENGIIVGRDRLARLRKELRLRC
                     KQKRKFRATTNPNHNLPVAPNLLNQTFAPTAPNQVWVADLTYVATQEGWLYLAGIKDV
                     YTCEIVGYAMGERMTKELT"
BASE COUNT           98 a           94 c          103 g           69 t
ORIGIN      
        1 agattaaggt ggcacatatc cgcactcgcg aaacatatgg aacccggcgg ctccagacgg
       61 agctggcaga gaatggcatc atcgttggtc gtgaccgact ggcacgtctt cgtaaggagc
      121 taaggctacg ctgtaagcag aaacgcaagt tcagagcgac tacgaacccg aaccacaatc
      181 tgccagttgc gccaaatctg ctgaaccaga cgttcgctcc tacagcacca aatcaggtct
      241 gggtggcgga cctgacgtat gttgccacac aggagggatg gttgtacctc gctggcatca
      301 aagatgttta tacgtgcgaa attgtcggct acgccatggg agagcgcatg acaaaagagc
      361 tgac
//
LOCUS       DADLFA010000412          362 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.414, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000412 DADLFA010000000
VERSION     DADLFA010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.414"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..355)
                     /locus_tag="JQH65_004201"
     CDS             complement(<1..355)
                     /locus_tag="JQH65_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAY6141383.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKQARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIK"
BASE COUNT           87 a           82 c           89 g          104 t
ORIGIN      
        1 gcttaatttt gctttcacat ttttgtaagt cttctgccgg attgtgcgac gtctgcaata
       61 tcacgtcgaa accttcctct tcagctttgg cggtgatggc atgcaaaact tcagagaaaa
      121 acggattacc cgccgtagtt ttggtcgaac gggtagaaat caccataatg gcatcaaaac
      181 ccgaagaggt cagcgcgcgg gcctgcttgt ttggctgata ctgtaattct tcaatggccc
      241 gtaacacttt ttcgcgcgct tccggggaga tattagtttg cttattcagc acgcgtgata
      301 cggtggattt tgatacgcct gcaaccctgg caatatcata aatagtaggg gacataggtc
      361 ag
//
LOCUS       DADLFA010000413          335 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.415, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000413 DADLFA010000000
VERSION     DADLFA010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.415"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            88..>335
                     /locus_tag="JQH65_004202"
     CDS             88..>335
                     /locus_tag="JQH65_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141384.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWL"
BASE COUNT          110 a           84 c           70 g           71 t
ORIGIN      
        1 ggctgcaatc aatgattatg gcggcagttc cgggacagaa actcgtccac tgcaataaca
       61 aatataaaaa acacaggtca tcagggaatg ccacaacgac accaccaggg acataaacgc
      121 acaccgaaac agttggcgct catcatcaaa cgctgtttgc cgatggtgct cactggcagc
      181 ggcatgcttt gcactaccgc taacgccgaa gagtattatt tcgaccccat tatgctggaa
      241 actacaaaaa gtggtatgca aacaactgat ctgtcacgtt tttcaaaaaa atacgcacaa
      301 ctaccaggaa cttatcaggt tgatatctgg ctgaa
//
LOCUS       DADLFA010000414          333 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.416, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000414 DADLFA010000000
VERSION     DADLFA010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.416"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            179..>333
                     /locus_tag="JQH65_004203"
     CDS             179..>333
                     /locus_tag="JQH65_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAY6141385.1"
                     /translation="MKRHNQRLKIISEYFGGVPVKNIGIREVYSFLEIRAAGSKFAIA
                     NQYRALLS"
BASE COUNT          125 a           49 c           74 g           85 t
ORIGIN      
        1 aatcggaaga gacaaaagaa ttgcagttaa tgaggctatt gcggcaaata tggaattatt
       61 atccacgcaa catataagct tactggatag aataaaaggt aatagcgtaa cattattctg
      121 cgagctgata ttaaatttca gaaatgaaat agagcgtaga caattaagca caaacaccat
      181 gaaaagacac aaccagaggt tgaaaatcat aagtgaatac tttggtggtg ttccggttaa
      241 aaatataggc ataagggagg tgtacagctt tcttgaaatc agagcagctg ggagtaaatt
      301 tgctattgct aaccagtaca gggcgctttt atc
//
LOCUS       DADLFA010000415          328 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.417, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000415 DADLFA010000000
VERSION     DADLFA010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.417"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(35..199)
                     /locus_tag="JQH65_004204"
                     /pseudo
     CDS             complement(35..199)
                     /locus_tag="JQH65_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135850.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
     gene            complement(186..>328)
                     /locus_tag="JQH65_004205"
     CDS             complement(186..>328)
                     /locus_tag="JQH65_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY6141386.1"
                     /translation="ISDEDKEKLKACRIYAKSLQAMDFSTITDKATYNAINWPERPDA
                     AA"
BASE COUNT           65 a           80 c           86 g           97 t
ORIGIN      
        1 aaatggtagt gtccgctatt gccagtacac ctcacacacc cggcagaacg gggaagtcat
       61 attccccgtt ctgtgtggtg acatggctga aaacactttt cagaatccct gcacggttgt
      121 gtatggaggt tagtgtaatg gttgcgccgt ccagcggttc tcccgtgggc gttttaatca
      181 cacctttaag ctgcggcatc gggacgctcc ggccagttga tggcgttata tgtggcttta
      241 tctgtgatgg tgctgaaatc catcgcctgc agcgatttcg cgtaaatacg acaggctttc
      301 agcttttctt tatcttcgtc gctgatta
//
LOCUS       DADLFA010000416          321 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.418, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000416 DADLFA010000000
VERSION     DADLFA010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.418"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..>321
                     /gene="ttdR"
                     /locus_tag="JQH65_004206"
                     /gene_synonym="dan"
     CDS             <1..>321
                     /gene="ttdR"
                     /locus_tag="JQH65_004206"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
                     /protein_id="HAY6141387.1"
                     /translation="LVDDVTQIKTRPEGMIRIGCSFGFGRSHIAPAITELMRNYPELQ
                     VHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAHLLTKNKRILCAAPEYLQKYPQPQS
                     LQEL"
BASE COUNT           96 a           60 c           71 g           94 t
ORIGIN      
        1 gactggtcga tgacgtcacg caaatcaaaa cgcgcccgga ggggatgatt cgtattggtt
       61 gcagttttgg ttttgggcgc agccatattg cgccagccat taccgaactt atgcgcaatt
      121 atcctgagtt acaggtgcat tttgaattgt tcgatcggca aattgattta gtgcaggata
      181 atattgatct ggatattcgt attaatgacg aaattcctga ttattatatt gcgcatctgt
      241 taacgaaaaa taaaagaata ttatgcgcag cacctgagta tctgcaaaaa tatccgcagc
      301 cacaatcatt acaggaatta a
//
LOCUS       DADLFA010000417          318 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.419, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000417 DADLFA010000000
VERSION     DADLFA010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.419"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..>318
                     /locus_tag="JQH65_004207"
     CDS             <1..>318
                     /locus_tag="JQH65_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAY6141388.1"
                     /translation="SFSPDSRLTMKALEMAWETRGKPGGVMFHSDQGSHYTSRQFRQL
                     LWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWMPVVGYVSFSEAAHAITDYIVGYYS
                     ALRP"
BASE COUNT           82 a           76 c           99 g           61 t
ORIGIN      
        1 gtcgttctcg ccggacagca ggctcaccat gaaagcgctg gaaatggcat gggaaacccg
       61 tggtaagccc ggcggggtga tgttccacag cgatcagggc agtcattata cgagcaggca
      121 gttccggcag ttattgtggc gataccagat cagacagagt atgagccggc gcggaaactg
      181 ctgggataac agcccaatgg aacgcttctt caggagtctg aagaacgaat ggatgccggt
      241 ggtgggttac gtaagcttca gcgaggcagc tcacgccata acggactata tcgttggata
      301 ttacagcgca ctaagacc
//
LOCUS       DADLFA010000418          317 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.420, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000418 DADLFA010000000
VERSION     DADLFA010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.420"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..>317
                     /locus_tag="JQH65_004208"
     CDS             <1..>317
                     /locus_tag="JQH65_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141389.1"
                     /translation="YPALVGNDIGCGMALWQTDILARKYNADKFEKRLSDLDDVAEES
                     WLEENLPSAFAQHPWCSSLGSIGGGNHFAELQQVDQIINAELFALAGLDAQHLQLLVH
                     SGS"
BASE COUNT           80 a           75 c           83 g           79 t
ORIGIN      
        1 tttatccggc actggtcggc aatgatatcg gctgcggtat ggcgctatgg caaacagata
       61 ttctcgctcg caaatacaac gccgataagt ttgaaaagcg attatcagat ctggatgacg
      121 ttgctgaaga aagctggctg gaggaaaacc tgccgtcagc atttgcacag catccgtggt
      181 gcagctcgct tggctctatc ggtggcggca atcatttcgc tgaactgcaa caagttgatc
      241 aaatcatcaa cgctgaactg tttgcactag caggtctgga tgcacaacat ctgcaactgt
      301 tggttcatag cggctcg
//
LOCUS       DADLFA010000419          315 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.421, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000419 DADLFA010000000
VERSION     DADLFA010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.421"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..>315
                     /locus_tag="JQH65_004209"
     CDS             <1..>315
                     /locus_tag="JQH65_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HAY6141390.1"
                     /translation="IQETGFDGRTQRYHHDLTGKLIRSEDEGLVTHWHYDEADRLTHR
                     TVNGETAEQWQYDKRGWLTDISHLSEGHRVTVHYRYDEKGRLTGERQTVHHPQPEALL
                     WQ"
BASE COUNT           81 a           94 c           97 g           43 t
ORIGIN      
        1 gatacaggaa accggctttg acggccgcac acagcgttat caccacgacc tgaccggcaa
       61 acttatccgc agcgaggatg aggggctggt cacccactgg cactatgacg aagcagaccg
      121 cctcacgcac cgcaccgtga atggcgaaac cgcagagcag tggcagtatg acaaacgtgg
      181 ctggctgaca gacatcagcc atctcagcga agggcaccgg gtgacggtgc attacaggta
      241 tgatgagaaa ggccggctga ccggtgagcg ccagacggtg catcacccgc agccggaagc
      301 actgctctgg cagca
//
LOCUS       DADLFA010000420          310 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.422, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000420 DADLFA010000000
VERSION     DADLFA010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.422"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(12..86)
                     /locus_tag="JQH65_004210"
     tRNA            complement(12..86)
                     /locus_tag="JQH65_004210"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(52..54),aa:Gln,seq:ttg)
     gene            complement(121..195)
                     /locus_tag="JQH65_004211"
     tRNA            complement(121..195)
                     /locus_tag="JQH65_004211"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(161..163),aa:Gln,seq:ttg)
     gene            complement(219..303)
                     /locus_tag="JQH65_004212"
     tRNA            complement(219..303)
                     /locus_tag="JQH65_004212"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(267..269),aa:Leu,seq:tag)
BASE COUNT           74 a           87 c           83 g           66 t
ORIGIN      
        1 tgtttcttct atggctgggg tacctggatt cgaaccaggg aatgccggta tcaaaaaccg
       61 gtgccttacc gcttggcgat accccaataa ccgggcggtg aaccgcttac tcgaagaaga
      121 tggctggggt acctggattc gaaccaggga atgccggtat caaaaaccgg tgccttaccg
      181 cttggcgata ccccatccgt acaacacttt ctggtgaatg gtgcgggagg cgagacttga
      241 actcgcacac cttgcggcgc cagaacctaa atctggtgcg tctaccaatt tcgccactcc
      301 cgccaaaaag
//
LOCUS       DADLFA010000421          308 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.423, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000421 DADLFA010000000
VERSION     DADLFA010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.423"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..263)
                     /locus_tag="JQH65_004213"
     CDS             complement(<1..263)
                     /locus_tag="JQH65_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAY6141391.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPG"
BASE COUNT           88 a           75 c           84 g           61 t
ORIGIN      
        1 cccggcagca tgatgtccag gcgattcaca atctggtgaa gaggttcttt acgctcaagc
       61 gccatgccaa caatacacca gaccatcatt tcgaggggaa gacggcgctt gcgtagcgtt
      121 acagtacctg attcggcaag gcaacgagag atgagttcgg ggtcgaggta atcccccaga
      181 gaagtcagtg ggttacgcag agaatcgtaa cgggatacca gatcaagagc ctgtccaatg
      241 tgcataaaaa aatccggaaa caagtgagcg tttccggatt cttacacagc cactggatcg
      301 gtcaactg
//
LOCUS       DADLFA010000422          295 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.424, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000422 DADLFA010000000
VERSION     DADLFA010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.424"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..>295)
                     /locus_tag="JQH65_004214"
     CDS             complement(<1..>295)
                     /locus_tag="JQH65_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY6141392.1"
                     /translation="SVRLTQSTRRLSEIGVIRVYEDSTDGSLNDFLGATNIDLRPESL
                     KKFEELAQQAQQSAGSAAGNARQTAQDMTAAATARDDAQRFAEKARQDATVTAE"
BASE COUNT           49 a           89 c           80 g           77 t
ORIGIN      
        1 ctcagctgtg acggttgcat cctgtcgggc tttctccgca aaacgttgtg catcatcacg
       61 ggccgttgcg gctgccgtca tatcctgcgc cgtctgccgt gcgtttccgg ctgcgctccc
      121 ggcactctgc tgcgcctgtt gcgccagctc ttcgaatttc ttcagggatt ccggacgcag
      181 gtcaatatta gttgcgccca gaaaatcatt cagcgaaccg tccgttgaat cttcataaac
      241 gcggatgaca ccgatttctg aaaggcgccg tgtgctttgt gtcaggcgaa cgctg
//
LOCUS       DADLFA010000423          289 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.denovo.425, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000423 DADLFA010000000
VERSION     DADLFA010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.denovo.425"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            7..>289
                     /locus_tag="JQH65_004215"
     CDS             7..>289
                     /locus_tag="JQH65_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005113674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY6141393.1"
                     /translation="MSGCQIILKSRVNTSEVVMRTRADVVTGNNGEYSFEAQVGKYCV
                     YLKRDWRDEYCVGDIAVYDDSKPGTLNDFLTAPDEGDLKPDVVKRFEEMV"
BASE COUNT           74 a           65 c           84 g           66 t
ORIGIN      
        1 atccccatgt ccggatgcca gattattctg aaatcccgtg taaacacctc agaagtggtg
       61 atgcgtacca gggctgatgt agtgaccgga aataacggtg aatattcgtt tgaggcacag
      121 gtcggaaaat attgcgtgta tctgaaacgg gactggcgcg acgagtactg tgttggcgac
      181 attgctgtat acgacgactc aaagcccggc acgctgaatg actttctgac tgcccccgat
      241 gaaggcgacc tgaagccgga tgtcgtcaaa cgctttgagg aaatggtgg
//
LOCUS       DADLFA010000424        11188 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.guided.135, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000424 DADLFA010000000
VERSION     DADLFA010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11188
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.guided.135"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(37..603)
                     /locus_tag="JQH65_004216"
                     /pseudo
     CDS             complement(37..603)
                     /locus_tag="JQH65_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(822..1799)
                     /gene="epmA"
                     /locus_tag="JQH65_004217"
     CDS             complement(822..1799)
                     /gene="epmA"
                     /locus_tag="JQH65_004217"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HAY6141394.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLNLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            2124..3932
                     /gene="frdA"
                     /locus_tag="JQH65_004218"
     CDS             2124..3932
                     /gene="frdA"
                     /locus_tag="JQH65_004218"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HAY6141395.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRMAGEQATERAATAGNGNEEAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3925..4659
                     /locus_tag="JQH65_004219"
     CDS             3925..4659
                     /locus_tag="JQH65_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HAY6141396.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4670..5065
                     /gene="frdC"
                     /locus_tag="JQH65_004220"
     CDS             4670..5065
                     /gene="frdC"
                     /locus_tag="JQH65_004220"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HAY6141397.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPDAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            5076..5435
                     /gene="frdD"
                     /locus_tag="JQH65_004221"
     CDS             5076..5435
                     /gene="frdD"
                     /locus_tag="JQH65_004221"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HAY6141398.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5498..6631
                     /gene="blaEC"
                     /locus_tag="JQH65_004222"
     CDS             5498..6631
                     /gene="blaEC"
                     /locus_tag="JQH65_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HAY6141399.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQMPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6720..7253
                     /locus_tag="JQH65_004223"
     CDS             6720..7253
                     /locus_tag="JQH65_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HAY6141400.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDGGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(7250..7567)
                     /locus_tag="JQH65_004224"
     CDS             complement(7250..7567)
                     /locus_tag="JQH65_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound-resistance protein
                     SugE"
                     /protein_id="HAY6141401.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7749..7895)
                     /gene="ecnB"
                     /locus_tag="JQH65_004225"
     CDS             complement(7749..7895)
                     /gene="ecnB"
                     /locus_tag="JQH65_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HAY6141402.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(8006..8131)
                     /gene="ecnA"
                     /locus_tag="JQH65_004226"
     CDS             complement(8006..8131)
                     /gene="ecnA"
                     /locus_tag="JQH65_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HAY6141403.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(8183..8749)
                     /gene="efp"
                     /locus_tag="JQH65_004227"
     CDS             complement(8183..8749)
                     /gene="efp"
                     /locus_tag="JQH65_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAY6141404.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8791..9840
                     /gene="epmB"
                     /locus_tag="JQH65_004228"
     CDS             8791..9840
                     /gene="epmB"
                     /locus_tag="JQH65_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HAY6141405.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRVGVTLLNQSVLLRDVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQLLIKQGS"
     gene            10214..11083
                     /locus_tag="JQH65_004229"
     CDS             10214..11083
                     /locus_tag="JQH65_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6141406.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEVEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
BASE COUNT         2934 a         2829 c         2787 g         2638 t
ORIGIN      
        1 atcatacact aaatcagtaa gttggcagca ttacccgaaa tgcccaccat ttaataacaa
       61 aatagtaatg cttactaaca gcaatactaa attcagacac attactgcaa taccgcccac
      121 cgcagtaatg cgggcaattt tattaatccc ctttgaagca acgacggtga ccagaatcat
      181 ccatgctact gccagcagac caaccacctg cgtaggctcc agtccggcaa tacgccagtg
      241 ctgggtcatg tcgctaccat agaggaatgt tgagaacggt acccaaactt tcgcggaggt
      301 actcaccatc cagatgatat aagaggaaaa ccacataaac gtaccaatga aggcaaaacg
      361 tggtccgaca ctattattca tccaggaata gataccacct tcttctttgc gataagcagc
      421 tcccatttca gccatcatta aggcgaatgg aataaagaat aacaatgcag aaaatatata
      481 aaagggaatc gcactataac ccattaagta ataagccgat gggctattgg caaatccaaa
      541 tacggaagta aagatcatca atatgagtcc tatcagactc atctttttta tcgtgtgagg
      601 catgaaacca tccttcattt cagcgcgtgt ataacacgtg cccactgaac tgcactgcgc
      661 aacattgagc aggcagcgta aaaaccaata cagaattgat acccggatga tatcaaatat
      721 ccagtcttaa attgtggcct ttaacggaaa aatgaaaaca tatgctacag aatggcgctt
      781 atcacgccat tcttcgctgt taattcagta atttttcaga attatgcccg gtcaacgcta
      841 aaggcgatga cttcagccag tgtctccgcg cccagcgcca acatcaccag acgatcaaca
      901 cctaatgcca cgccggaaca gtcaggcata ccgactttca aggcttcaat cagattctgg
      961 tcaatggggt gctgcggcaa accgcgcgcc gcgcgcttac ggttatcttg ttcaaagcgt
     1021 tgttgctgct cacgggcatc cgttaattca tggaaaccat tcgccagctc aataccttta
     1081 taataaacct caaagcgttc agcgacccga tgatcttcgg tactgatttg cgccagtgat
     1141 gcctggctgg ctggaaagtg gtacacaaag gtcggttttt ctttgccaat atttggctct
     1201 acgccaaagg taaacagcaa ttgtagcaac gtgtcgcggt cttcttcggt atcagcaacg
     1261 ttactcaaat tcagtttcgc tgcgacttcc cgcagttgcg ttttgtcggc agagagcggg
     1321 tcaatttcca gataacgcaa gaaagcttgt tgataagaaa ggctttctgc tgccgggcag
     1381 tccagcacct gttgtaagag atcgtccacc tcgttcatca accggtacat atcatagtgc
     1441 ggtcgatacc actcaagcat agtgaactca gggttgtgat aacgccccat ctcttcatta
     1501 cggaagctgc ggcacagctg gaataccggc ccacaaccgg caaccagcag gcgtttcata
     1561 tggtattccg ggctggtcat taaccagaga ttcatcccct gcgaatgccc ggggccaacg
     1621 aaacgtgtct caaacgggac caaatgaata tcggttaccg tcgcctggct catacaaggc
     1681 gtctccacct ccagcactcc acgatcagca aagaaacgac ggatctccgc cataatcgcc
     1741 gcgcgtttta ataagttagg aatggatgcg ctcggctgcc aggatgccgt ttcgctcata
     1801 gttaaatctc cagtttttga caagggcacg aagtctactc gcaacgcgac ggcgagacaa
     1861 attttacgca ggaatcaaac agcggtgggc agtgactaaa aaaagcacga tctgatggtt
     1921 tagtaattaa attaatcatc ttcagtgata atttagccct cttgcgcact aaaaaaatcg
     1981 atctcgtcaa atttcagact tatccatcag actatactgt tgtacctata aaggagcagt
     2041 ggaatagcgt tcgcagaccg taactttcag gcacttaccc tgaagtacgg ggctgtggga
     2101 taaaaacaat ctggaggaat gtcgtgcaaa cctttcaagc cgatcttgcc attgtaggcg
     2161 ccggtggcgc gggattacgt gctgcaattg ctgccgcgca ggcaaatcca aatgcaaaaa
     2221 tcgcactaat ctcaaaagta tacccgatgc gtagccatac cgttgctgca gaagggggct
     2281 ccgccgctgt cgcgcaggat catgacagct tcgaatatca ctttcacgat acagtagcgg
     2341 gtggcgactg gttgtgtgag caggatgtcg tggattattt cgtccaccac tgcccaaccg
     2401 aaatgaccca actggaactg tgggggtgcc catggagccg tcgcccggat ggtagcgtca
     2461 acgtacgtcg cttcggcggc atgaaaatcg agcgcacctg gttcgccgcc gataagaccg
     2521 gcttccatat gctgcacacg ctgttccaga cctctctgca attcccgcag atccagcgtt
     2581 ttgacgaaca tttcgtgctg gatattctgg ttgatgatgg tcatgttcgc ggcctggtag
     2641 caatgaacat gatggaaggc acgctggtgc agatccgtgc taacgcggtc gttatggcta
     2701 ctggcggtgc gggtcgcgtt tatcgttaca acaccaacgg cggcatcgtt accggtgacg
     2761 gtatgggtat ggcgctaagc cacggcgttc cgctgcgtga catggaattc gttcagtatc
     2821 acccaaccgg tctgccaggt tccggtatcc tgatgaccga aggctgccgt ggtgaaggcg
     2881 gtattctggt caacaaaaat ggctaccgtt atctgcaaga ttacggcatg ggcccggaaa
     2941 ctccgctggg cgagccgaaa aacaaatata tggaactggg tccacgcgac aaagtttctc
     3001 aggccttctg gcacgaatgg cgtaaaggca acaccatctc cacgccgcgt ggcgatgtgg
     3061 tttatctcga cttgcgtcac ctcggcgaga aaaaactgca tgaacgtctg ccgttcatct
     3121 gcgaactggc gaaagcgtac gttggcgtcg atccggttaa agaaccgatt ccggtacgtc
     3181 cgaccgcaca ctacaccatg ggcggtatcg aaaccgatca gaactgtgaa acccgcatta
     3241 aaggtctgtt cgccgtgggt gaatgttcct ctgttggtct gcacggtgca aaccgtctgg
     3301 gctccaactc cctggcggaa ctggtggtct tcggccgtat ggccggtgaa caagcgacag
     3361 agcgtgcagc aactgccggt aatggcaacg aagaggcaat tgaagcgcag gcagctggcg
     3421 ttgaacaacg tctgaaagat ctggttaacc aggatggcgg cgaaaactgg gcgaagatcc
     3481 gcgacgaaat gggcctagca atggaagaag gttgcggtat ctaccgtacg ccggaactga
     3541 tgcagaaaac catcgacaag ctggcagagc tgcaggaacg cttcaagcgc gtgcgcatca
     3601 ccgacacctc cagcgtgttc aacaccgacc tgctctacac cattgaactg ggccacggtc
     3661 tgaacgttgc tgaatgtatg gcgcactccg caatggcacg taaagagtcc cgcggcgcac
     3721 accagcgtct ggacgaaggt tgcaccgagc gtgacgacgt caacttcctc aaacacaccc
     3781 tcgccttccg cgatgctgat ggcacgactc gcctggagta cagcgacgtg aagattacta
     3841 cgctgccgcc agctaaacgc gtttacggtg gcgaagcgga tgcagccgat aaggcggaag
     3901 cagccaataa gaaggagaag gcgaatggct gagatgaaaa acctgaaaat tgaggtggtg
     3961 cgctataacc cggaagtcga taccgcaccg catagcgcat tctatgaagt gccttatgac
     4021 gcaactacct cattactgga tgcgctgggc tacatcaaag acaacctggc accggacctg
     4081 agttaccgct ggtcctgccg tatggcgatt tgtggttcct gcggcatgat ggttaacaac
     4141 gtgccaaaac tggcatgtaa aaccttcctg cgtgattaca ccgacggtat gaaggttgaa
     4201 gcgttagcta acttcccgat tgaacgcgat ctggtggtcg atatgaccca cttcatcgaa
     4261 agtctggaag cgatcaaacc gtacatcatc ggcaactccc gcaccgcgga tcagggtact
     4321 aacatccaga ccccggcgca gatggcaaag tatcaccagt tctccggttg catcaactgt
     4381 ggtctgtgct acgccgcgtg cccgcagttt ggcctgaacc cagagttcat cggtccggct
     4441 gccattacgc tggcgcatcg ttataacgaa gatagccgcg accacggtaa gaaggagcgt
     4501 atggcgcagt tgaacagcca gaacggcgta tggagctgta ctttcgtggg ctattgctcc
     4561 gaagtctgcc cgaaacacgt cgatccggct gcggccattc agcagggcaa agtagaaagt
     4621 tcgaaagact ttcttatcgc gaccctgaaa ccacgctaag gagtgcaaca tgacgactaa
     4681 acgtaaaccg tatgtacggc caatgacgtc cacctggtgg aaaaaattgc cgttttatcg
     4741 cttttacatg ctgcgcgaag gcacggcggt tccggctgtg tggttcagca ttgaactgat
     4801 tttcgggctg tttgccctga aaaatggccc ggacgcctgg gcgggattcg tcgacttttt
     4861 acaaaacccg gttatcgtga tcattaacct gatcactctg gcggcagctc tgctgcacac
     4921 caaaacctgg tttgaactgg caccgaaagc ggccaatatc attgtaaaag acgaaaaaat
     4981 gggaccagag ccaattatca aaagtctctg ggcggtaact gtggttgcca ccatcgtaat
     5041 cctgtttgtt gccctgtact ggtaaggagc ctgagatgat taatccaaat ccaaagcgtt
     5101 ctgacgaacc ggtattctgg ggcctcttcg gggccggtgg tatgtggagc gcaattattg
     5161 cgccggtgat gatcctgctg gtgggtattc tgctgccact ggggctgttt ccgggtgatg
     5221 cgctgagcta cgagcgcgtt ctggcgttcg cgcagagctt cattggtcgc gtattcctgt
     5281 tcctgatgat cgttctgccg ctgtggtgtg gtttacaccg tatgcaccac gcgatgcacg
     5341 atctgaaaat tcacgtgcct gcgggcaaat gggttttcta cggtctggct gctatcctga
     5401 cagttgtcac gctgattggt atcgttacaa tctaacgtat cgccaatgta aatccggccc
     5461 gcctatggcg ggccgttttg tatggaaacc agaccttatg ttcaaaacga cgctctgcgc
     5521 cttattaatt accgcctctt gctccacatt tgctgcccct caacaaatca acgatattgt
     5581 gcatcgcaca attaccccgc ttatagagca acaaaagatc cccggtatgg cggtggcggt
     5641 aatttatcag ggtaaacctt attactttac ctggggctat gcggacatcg ccaaaaagca
     5701 gcccgtcaca cagcaaacgt tgtttgagtt aggttcggtc agcaaaacat ttacgggcgt
     5761 gcttggtggc gatgctattg ctcgagggga aatcaagtta agcgatcccg caacaaaata
     5821 ctggcctgaa cttaccgcta aacagtggaa tgggatcaca ctattacatc ttgcgaccta
     5881 caccgctggc ggcctgccat tgcagatgcc ggatgaagtg aaatcctcaa gcgacttgct
     5941 gcgcttctat caaaactggc agcctgcatg ggctccagga acacaacgtc tgtatgccaa
     6001 ctccagtatc ggtttgttcg gcgcactggc tgtgaagccg tctggtttga gttttgagca
     6061 ggcgatgcaa actcgtgtct tccagccact caaactcaac catacgtgga ttaatgtacc
     6121 gcccgcagaa gaaaagaatt acgcctgggg atatcgcgaa ggcaaggcag ttcatgtttc
     6181 gccaggggcg ttagatgctg aagcttatgg tgtgaagtcg accattgaag atatggcccg
     6241 ctgggtgcaa agcaatttaa ccccccttga tatcaatgag aaaacgcttc aacaagggat
     6301 acaactggca caatctcgct actggcaaac cggcgatatg tatcagggcc tgggctggga
     6361 aatgctggac tggccggtca atcctgacag catcattaac ggcagtgaca ataaaattgc
     6421 actggcagca cgcccggtaa aagcgattaa gcccccaact cctgcagtac gcgcatcatg
     6481 ggtacataaa acgggggcga ccggcggatt tggtagctat gtcgcgttta ttccagaaaa
     6541 agagctgggt atcgtgatgc tggctaacaa aaactatccc aatccagcga gagtcgccgc
     6601 cgcctgtcag attctcaacg ctctacagta aaatttcatc gggtccgaat tttcggacct
     6661 tttctgcgct tttccttgct gtcatctaca cttagaaaaa aaccagtaag gaaacattta
     6721 tgcgcctgct ccctctcgtt gccgcagcga cagctgcatt tctggtcgtt gcctgcagtt
     6781 ctcctacgcc gccgcgtggc gtgacagtag tgaataattt cgacgctaaa cgctatctcg
     6841 gtacctggta tgagattgcc cgttttgatc accgttttga acgtggactg gaaaaagtca
     6901 ccgcaacata cagtctgcgt gatggcggcg gcctgaatgt catcaataaa ggctataacc
     6961 ctgacagaga aatgtggcag cagagtgaag ggaaagcgta ctttaccggc gcaccaacac
     7021 gcgctgcgct gaaagtgtca ttctttggtc ctttctatgg cggttataac gtcattgcac
     7081 ttgatcggga ataccgccat gcgctggttt gcggcccgga ccgcgactac ctgtggatac
     7141 tctcccgcac gccaaccatt tctgacgaag tgaaacagga gatgctggca gtcgcgaccc
     7201 gggaagggtt tgatgtcagt aagtttattt gggtacagca gcccggtagt tagtgagtgc
     7261 tgagtttcag accaataatc cccaatacga ttaacgccag actcgccagg cgcatcgggt
     7321 tagcggactc accgagcagc acaatgccgg tgatagccgc gccgactgcg ccaatacccg
     7381 tccacacggc ataagccgtc cctactggta acgatttcat cgcccaggca agtagcgcca
     7441 tactgacaat catcgccgtc acggtaataa cactcggcgt caaacgacta aagccgtggg
     7501 tatatttcag gccaacggcc cataccactt ccagcagacc agcaataact aagataatcc
     7561 aggacatatc aggctccgga gaattggggc cgtccccggt gaaaagacgc gtttgcaggt
     7621 cgtcctgcaa agctgatggt tgaacggata ttttgccagg tggcagggga atttcaacta
     7681 ttaccttagt gactatcgca gaaaaaacaa acggcacgac acagctgtcg taccgttatt
     7741 gcttattgct attgctgcgc tttcgttgct gcgccagaaa tcgcgttacc gccatcagaa
     7801 atgtcttcac caacgccacg cgtggtgttg caggcagtta atactgttga aagcaccaga
     7861 acagaaaaga tcgctgcaat tgtcttcttc accataacgt cttcctttta ggtaatgtta
     7921 tttatgtttg cctatagcaa attaataata gacaacatcg ccaaaaatga cggaaatctg
     7981 atgatttttc ggaagagaag aaaaattagc tggcagcgcg agagatggag ttgccgagat
     8041 gtttgatgtc ttcgccgaaa ccgcgagcgg tgttacagcc cgtgagcagc gtactggcaa
     8101 gcaaaacaag aacgataagg cgtttcatca tctctgacct gaataagtga tggtgcagcc
     8161 tgcaggccgc accacaaccg cattacttca cgcgagagac gtattcacca gagcgggtat
     8221 ccactttgat gacttcgccg atttgtacga acagcggaac cttaaccaca gcgccagtag
     8281 acagggtagc cggtttgccg ccagtacctg cggtatcacc tttcaggccc ggatcggtat
     8341 caacgatttc cagttcaacg aagttcggcg gagtaacgga gatcggctga ccattccaca
     8401 gagttacgat acactctgcc tgatccagca gccatttagc gttatcgcca attgcttttg
     8461 catcagcaga cagctgctcg aaagtttcgt tgttcatgaa gtgccagaac tcaccgtcgt
     8521 tgtacaggta agtcaggttc atatcgacaa catcagcgcc ttcagcggaa tcagtagatt
     8581 tgaaggtttt ttctacgcga gtaccggtca gcagacgacg cagtttaacg cgagcaaatg
     8641 cctggccttt acccggtttt acgaattcac tcgcttcaac cgcgtaaggt tcgccgtcta
     8701 acatgatttt aagaccagca cgaaaatcgt tgctatagta cgttgccata aggccctctg
     8761 aaatttgtta attggtagct aagccacaaa atggcgcata ttgtaaccct aaatacccca
     8821 tccagagaag attggttaac gcaacttgcc gatgttgtga ccgatcctga tgaacttctg
     8881 cgtcttttga atatagacgc ggacgaaaaa ctgttggccg gacgcagcgc caaaaagctg
     8941 tttgccctgc gtgtgccccg ctcatttatc gatcgcatgg agaaaggcaa tccggacgat
     9001 cctcttttgc gtcaggtact tacctcgcaa gatgagtttg tcgtcgcgtc cggattctcc
     9061 accgacccac tggaagaaca gcacagcgta gtgcctggtt tgttgcataa ataccacaac
     9121 cgggcgcttt tgctggtcaa aggcggctgc gcggtaaatt gccgctattg cttccgtcgc
     9181 cacttcccct atgccgaaaa tcagggcaac aagcgtaact ggcagactgc acttgagtat
     9241 gttgctgcgc atccggaact ggacgagatg attttctccg gcggcgatcc gctgatggcg
     9301 aaagatcacg agctggactg gttgctcaca caactggaag ccatcccgca tatcaaacgt
     9361 ctgcggattc acagtcgtct gccgattgtg atcccggcac gcatcacaga tgcgctggtt
     9421 gaacgctttt cacattctac gctgcaaatc ctgctggtga atcacatcaa ccatgccaat
     9481 gagatagatg aaacattccg tcaggcgatg gctaagttgc gccgggtagg cgttactttg
     9541 ctgaaccaga gcgttctgtt acgtgatgtg aacgataacg cacaaacgct ggcaaacctg
     9601 agtaatgcgt tgttcgatgc cggcgtgatg ccgtattacc tgcatgtgct cgataaagta
     9661 cagggcgcag cgcattttat ggtgagtgat gacgaagcac ggcagattat gcgtgagttg
     9721 ctgacattgg tgtcgggata tctggtgccg aaactggcgc gagaaattgg cggcgaaccc
     9781 agcaaaacgc cgctggatct ccagctacgc cagcagttac tgattaaaca gggaagttaa
     9841 acattaaaat tgcctgatgc gctacgctta tcaggcctac atcgtgcatt gaaattgaat
     9901 ggatttgcaa tgaactgtag gccggataag gcgtttacgc cgcatccggc aagagcaaca
     9961 aacgcaagta cagcaacctg tcattaaact gagaaattta atctttgccc gttgcataac
    10021 ctttaattat tcatatgcat tgctgttgtt actttttata tagctaacac caataattaa
    10081 cctttctttt ctgtttatat agagtgagca cggctattta tgttatttta acaactatta
    10141 ctaccaagcc catttatctg gcttataaat taaaattaat tatttaattg agaaatggtt
    10201 agggagaacc tacatggcca taagcatcaa gggtgttaat accggtgtta ttcgcaagag
    10261 taataatttc attgcactgg cgctgaaaat taaagaacct cgcaataaag aatcgttgtt
    10321 ctttatgtct gtcatggaac tgcgcgacct gctgattgcg ctggaaagtc gtctgcatca
    10381 aaagcataaa ctggatgctg ctgcacgcct tcagtatgag caagctcgcg ataaagtcat
    10441 taagaaaatg gcagaaaaca tcccagaaat tctggttgat gagcttaaaa atgccgatat
    10501 taatcgtcgg gttaacacgc tggaacttac cgataatcaa ggcgaaaatc ttaccttcgt
    10561 attaacgcta catgacggta gcaaatgtga gttagtcgtc aatgagttgc agattgaaat
    10621 gctagcgcga gcaatcattc atgcaattaa taatgcggaa atgcgtgaac tggcattacg
    10681 tattacctca ctgttagatt tcctgccgtt atatgacgtt gactgccagg aaaatggcaa
    10741 tctggaatat gacacctatt cccaaccgga atggaaacat aacttgttcg accattatct
    10801 ggcggtgctt taccgcttta aagatgaaag tggcaaagaa caatttagcg gcgcagtggt
    10861 gaaaacgcgt gaagcaacgc cgggtaaaga agtagaggcg atcactcgcc gaatgcttga
    10921 tttcagccca cgactgaaaa aactcgcggg tgtgccatgt caggtctatg ttcgcaccgt
    10981 agcggcgaat aacgcccaac cgctaaccca ggatcaatgc ttacgcgcgc tgcatcactt
    11041 acgtgttcaa tccaccagca aaactgcacc acaggcgaaa taatctcatc tttgctaatg
    11101 cgaggctgga gccatccagc ctgaaatgac tgataacgtg atcgttggtc atgccggatg
    11161 cggcgtaaac gccttatccg gcctacaa
//
LOCUS       DADLFA010000425        10234 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella boydii isolate MH160061
            SAMEA55357168-rid11426253.guided.148, whole genome shotgun
            sequence.
ACCESSION   DADLFA010000425 DADLFA010000000
VERSION     DADLFA010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55357168
            Sequence Read Archive: ERR1810573
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943519.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 19:04:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 168 of 295
            Pseudo Genes (incomplete)         :: 69 of 295
            Pseudo Genes (internal stop)      :: 92 of 295
            Pseudo Genes (multiple problems)  :: 31 of 295
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10234
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55357168-rid11426253.guided.148"
                     /isolate="MH160061"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..651
                     /locus_tag="JQH65_004230"
     CDS             <1..651
                     /locus_tag="JQH65_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HAY6141407.1"
                     /translation="HPIIRGTNKVTGQSILQRHIASFSHHGLPQGSDDALRYIAEHDD
                     ALAFACINRQMIALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNG
                     LVIIPGSRGDYSWIVQPVANEKTLHSLAHGAGRKWGRTECKWRLAAKYTVTQLSRTEL
                     GSRIICRDKQLIFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            648..1262
                     /locus_tag="JQH65_004231"
     CDS             648..1262
                     /locus_tag="JQH65_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HAY6141408.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1319..2776)
                     /gene="pepD"
                     /locus_tag="JQH65_004232"
     CDS             complement(1319..2776)
                     /gene="pepD"
                     /locus_tag="JQH65_004232"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008783788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HAY6141409.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNSNKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3037..3495
                     /gene="gpt"
                     /locus_tag="JQH65_004233"
     CDS             3037..3495
                     /gene="gpt"
                     /locus_tag="JQH65_004233"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAY6141410.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3587..4831
                     /gene="frsA"
                     /locus_tag="JQH65_004234"
     CDS             3587..4831
                     /gene="frsA"
                     /locus_tag="JQH65_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HAY6141411.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIVMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4889..5290
                     /locus_tag="JQH65_004235"
     CDS             4889..5290
                     /locus_tag="JQH65_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HAY6141412.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5329..6384)
                     /gene="phoE"
                     /locus_tag="JQH65_004236"
     CDS             complement(5329..6384)
                     /gene="phoE"
                     /locus_tag="JQH65_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HAY6141413.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGGEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAIGMTYQF"
     gene            6672..7775
                     /gene="proB"
                     /locus_tag="JQH65_004237"
     CDS             6672..7775
                     /gene="proB"
                     /locus_tag="JQH65_004237"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAY6141414.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7787..9040
                     /gene="proA"
                     /locus_tag="JQH65_004238"
     CDS             7787..9040
                     /gene="proA"
                     /locus_tag="JQH65_004238"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAY6141415.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTCDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9155..9230
                     /locus_tag="JQH65_004239"
     tRNA            9155..9230
                     /locus_tag="JQH65_004239"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9188..9190,aa:Thr,seq:cgt)
     gene            complement(9245..>10234)
                     /locus_tag="JQH65_004240"
     CDS             complement(9245..>10234)
                     /locus_tag="JQH65_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY6141416.1"
                     /translation="QIKHSPGNPGRFTHKKSLDDDKSRIGFWLQHFAGMQPRDITESK
                     IYSAMQKMTNRRHEENWKLRAEACRKKGKPVPEYTPKPASVATKATHLSFIKALLRAA
                     EREWKMLDKAPIIKVPQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSN
                     IINLEWQQIDMQRRVAWINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKES
                     CTKPDGTKAPTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARQIDSILNPSVPNLSQSRNKERTNDV"
BASE COUNT         2449 a         2516 c         2670 g         2599 t
ORIGIN      
        1 caccccatta taaggggtac gaataaagta acggggcagt ctattttaca gcggcatatt
       61 gcctcgttct cgcatcatgg tttgcctcaa ggcagtgacg acgcgctacg gtatattgcg
      121 gaacatgacg atgcgctggc gtttgcgtgt attaatcgcc agatgatcgc tttgcgcata
      181 atgcaacaag ttaaggccac gggaagtccg gttctggatg tggcgcataa ctttgttagc
      241 gcgtgtcaaa tcggcgatca acagggctgg ttgcatcgta aaggtgccac gccggatgac
      301 aacggtctgg tgattattcc cggttcacgt ggtgattact cctggattgt acagcctgtt
      361 gcgaacgaaa aaacgctcca ctcgctggcg catggggcag ggcgtaaatg ggggcgcacc
      421 gagtgtaaat ggcgtctggc agcgaaatac acggtgacgc aactctcgcg gactgaactt
      481 ggcagtcgga taatttgtcg cgataaacaa ctcatctttg aagaagcgcc acaagcctat
      541 aaatcggctg aaagcgtggt gcaatgtctg gtgcaggccg gtttaattat tcctgtcgcg
      601 cggctgcgtc cggtgctaac gctcaaaaac agtggaggga aaaaaggatg atcttgctac
      661 aactctcctc agcgcagggg ccggaagaat gttgtctcgc agtgaaaaag gcgctggaca
      721 ggctaattaa agaagctgcc cgacaagacg tcgcggtaac ggtgttggaa acagaaacgg
      781 gccgctactc tgacacgctg cgttcagctc tggtttctct ggatggcgat aacgcatggg
      841 cattaagcga aagctggtgt ggcactattc agtggatttg tccgagtccg tatcggcctc
      901 atcatgggcg caaaaactgg tttctgggca ttgggcgttt taccgctgat gagcaggagc
      961 aatccgatgc gatccggtat gagacgctgc gttcgtcggg gccgggcggt caacatgtca
     1021 ataaaaccga ctcggcggta cgcgccacgc atttggcatc cggtattagc gtgaaggttc
     1081 agtcagagcg tagtcagcat gctaacaagc ggctggcacg attgctgatt gcctggaagc
     1141 tggagcagca gcaacaggaa aatagcgcgg tgctgaaatc gcagcggcga atgttccatc
     1201 accagattga acgtggcaac ccgagacgga catttacagg gatggctttt atcgaaggat
     1261 aatgaaggat gaaactgccg gaaggcgatt aaacgccatc cggcagcaaa tcaaataatt
     1321 acttcgccgg aatttctttc agcagttcag tcagcagtgt ccagtaatga cctacgcttt
     1381 cgatgtgaac ttgctcatcc ggagagtgtg gaccggtgat agttggcccg atagaaacca
     1441 tgtccatttc cggatacggt tttttgaaca gaccacattc caggcccgcg tggataatct
     1501 ggatgttcgg cgtcttgttg aacaggcgct gataggtttc acgtaccaga tgcatcaccg
     1561 gagaattagc gtccggctgc cagccaggat atgcgccttt cgcttcggtt ttcgcgccag
     1621 ccagtttacc cagcgaatcc agcatgctca ccacgtagtc tttaccgctg tcgatcagtg
     1681 aacggatcag gcagtgaatt tctacgttat tgtcagtcat ggtcaccaca ccgacgttca
     1741 gggaggtttc aaccacacct ttggctacat cggagttgcg gatcacaccg ttcggggtgg
     1801 cgttcagcag acgaataaag gtatcgcgag atttcgcaat cagggcagct ttatcgttcg
     1861 ctacagagtc cagcaacaag gccagatttt tctctttctc tgccagctcg tttttcagga
     1921 tctcctgata ggtattcacc agagatttca gtgcgtcgac tttatcagct gcgacagcaa
     1981 tggtcgcaaa ggcttcacgc gggatggcgt tacgcagtgt gccgccgttg aaatcgataa
     2041 ggcgcaggtc cagttcttct gcatgacccg ccaggaagcg caccagcagt ttgttggaat
     2101 tacccaggcc aacgtggatt tcaccgccgg agtgaccgcc tttcagacct tttaaggtta
     2161 acttgaaggt ttcaaaacca gctggaaccg cttcacgatc taaatgcagg ttggaggtga
     2221 agtcgatacc ccccgcacaa cccatgtaga tttcaccttc ttcttcggag tcggtgttaa
     2281 tcagaatatc agcctgcaac cagttgctct gtaagccaaa agcaccgtcc ataccggctt
     2341 cttcggtcat ggtcagcagc acttccagcg gaccgtgaac cacgttttcg tcagccagaa
     2401 ccgccagtgc agaggccata ccaatgccgt tatccgcacc cagcgtggtg ccgcgcgctt
     2461 taacccattc gccatcaata taaggctgga taggatcttt cgtgaagtca tgcacggtgt
     2521 cgttattttt ctgcggcacc atatcgaggt gggcctgtaa gacgaccggt ttacgatttt
     2581 ccatacctgc ggtagcaggt ttacgaatca ggatattacc tacctgatcg cgttcgacat
     2641 ggaaaccttt ctcttttgcc caaccaacaa tgtattcagc gagttgctct tcatgatagg
     2701 acgggtgcgg aatagaacag attttggcaa aaatatccca cagcggctgt ggagataatt
     2761 gagacagttc agacacgtta agtctccttg tcgatcaccc gcaaaacagt attgcaggtc
     2821 acagggttag cagaaaatgt tgtcaacaca agacaggctt gcgagatatg tttgagaata
     2881 ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc atgtgaaata
     2941 ctggttttta gtgcgccaga tctctataat ctcgcgcaac ctattttccc ctcgaacact
     3001 ttttaagccg tagataaaca ggctgggaca cttcacatga gcgaaaaata catcgtcacc
     3061 tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa
     3121 tggaaaggca ttattgccgt aagccgtggc ggtctggtac cgggtgcgtt actggcgcgt
     3181 gaactgggta ttcgtcatgt cgataccgtt tgtatttcca gctacgatca cgacaaccag
     3241 cgagagctta aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat agttattgat
     3301 gacctggtgg ataccggtgg tactgctgtt gcgattcgtg aaatgtatcc aaaagcgcac
     3361 tttgtcacca tcttcgcaaa accggctggt cgtccgctgg ttgatgacta tgttgttgat
     3421 attccgcaag atacctggat cgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca
     3481 atctccggtc gctaatcttt tcaacgcctg gcactgccgg gcgttgttct ttttaacttc
     3541 aggcaggtta caatagtttc cagtaagtat tctggaggct gcatccatga cacaggcaaa
     3601 cctgagcgaa accctgttca aaccccgctt taaacatcct gaaacctcga cgctagtccg
     3661 ccgctttaat cacggcgcac aaccgcctgt gcagtcggct cttgatggta aaaccatccc
     3721 tcactggtat cgcatgatta accgtctgat gtggatctgg cgcggtattg acccacgtga
     3781 aatcctcgac gtccaggcac gtattgtgat gagcgatgct gaacgtaccg acgatgattt
     3841 atacgatact gtgattggct accgtggtgg caactggatt tatgagtggg ccacccaggc
     3901 gatggtgtgg caacaaaaag cctgtgcgga agaagatccg caactcagtg gtcgacactg
     3961 gctgcatgcg gctacgttgt acaacattgc cgcctatcct catctgaaag gagatgacct
     4021 ggccgagcaa gcgcaggctt tatcaaaccg cgcctatgaa gaggccgccc agcgtctgcc
     4081 gggcacgatg cggcagatgg agtttaccgt acccggcggt gcgcccatca ccggcttttt
     4141 gcatatgccg aaaggcgatg gcccgttccc gacagtatta atgtgtggtg gtctggatgc
     4201 gatgcagacg gactattaca gcctgtatga acgttatttt gcgccgcgcg gcattgtgat
     4261 gctgactatt gatatgccgt cggtgggctt ttcttcaaaa tggaagctca cccaggactc
     4321 cagcctgttg catcagcacg tcttaaaggc gctgcctaac gtaccgtggg tggatcacac
     4381 tcgcgtcgcg gcctttggtt tccgtttcgg cgctaacgtt gccgtgcgtc tggcatatct
     4441 tgaatcgccg cgtctgaaag cggttgcctg tcttggtccg gtagttcata ccctgttgag
     4501 tgattttaag tgccagcaac aggtgccgga aatgtatctt gacgttctgg cgagtcgttt
     4561 ggggatgcat gatgcttccg acgaagcgtt acgcgtggag ctgaatcgct attcattaaa
     4621 agtgcaagga ttgctgggac gtcgctgccc aacgccaatg ttatcaggct actggaagaa
     4681 cgatccgttc agcccggaag aggactcacg cttaatcacc tcatcatctg ctgacggtaa
     4741 attattagag atcccattta acccggtgta tcggaatttt gacaaaggtc ttcaggaaat
     4801 caccggctgg atcgaaaaac gcttgtgtta aaaatttgct aaattttgcc aatttggtaa
     4861 aacagttgca tcacaacagg agatagcaat gacgttaccg agtggacacc cgaagagcag
     4921 attgatcaaa aaatttaccg cactaggccc gtatattcgt gaaggtaagt gcgaagataa
     4981 tcgattcttt ttcgattgtc tggctgtatg cgtcaacgtg aaaccggcac cggaagtgcg
     5041 tgaattctgg ggctggtgga tggagctgga agcgcaggaa tcccgtttta cctacagcta
     5101 ccagtttggt ctgttcgata aagcaggcga ctggaagagt gttccggtaa aagatactga
     5161 agtggttgaa cgactggagc acaccctgcg tgagtttcac gagaagctgc gtgaactgct
     5221 gacgacgctg aatctgaagc tggaaccggc ggatgatttt cgtgacgagc cggtgaagtt
     5281 aacggcgtga gtgaaatgtg ccggatgcat cacatccggc aatatctatt aaaactgata
     5341 cgtcatccca atcgcgacaa tatcatcatt attaatattc aatttgttat cgctatccag
     5401 ttggttgatt ttataatcaa caaatgctga catatttttg ttgaaataat acgtagcacc
     5461 gacgtcgata taattaacca gatcttcacc accgatgccc tcaatatctt tccctttcga
     5521 taagacataa cccagcgatg gacgcagacc aaagtcaaac tggtattgag cgaccgcttc
     5581 aaagttctgt gtcttattgg caaagccgcc agttattggc gtcattttgc gagtttcaga
     5641 atagaaagtt gccagataaa tattattggc atcgtatttc agacctgttg cccatgcttc
     5701 tgcacgcttg cctgtgccac ggctttgcag gttctgctcg ttggtgcgat ctgagttggt
     5761 ataggcccca ctaatggcga aatcgctgcc gccaaagtca tatgtcaatg acgtgccgaa
     5821 gccatcgccg ttttgctttt taacgtcgcg gttttcgttt ttcccttgat attgcagggt
     5881 taagttcagg ccatcgataa cgccgaagaa gtcggtgttc cgatacgtcg ccagaccgct
     5941 ggcgcgtttg gtcataaagt tgtcggtctg cgccgaggag tcgccaccaa attccggaaa
     6001 catatcggtc caggcttcca cgtcatacaa cgcgcccagg ttacgaccat aatcgaaaga
     6061 acccaaatct ttatatttca acccggcaaa agcgagacgc gttttttgct gtgcagtatc
     6121 actctctgct ttattaccgg caaactctgc ttcccaacga ccataaccag tcagttgatc
     6181 gttaatttgt gtttcgcctt taaaaccaaa acggatataa ctctggtcgc catctttact
     6241 gtcgttatca ctcatataat gcatggcttt aactttgcca tagacatcca gtttattacc
     6301 gtctttgtta tatatttctg cggcctgtac ggatgcagat gccacaatgc ccatcaccac
     6361 taatgccaga gtgctctttt tcattttcat tcctgatttt aattaacgcg cgaatattca
     6421 gcgggagagc cccgttgaaa acaggaaagt ttttaacctg agattgttaa agatatatta
     6481 cagattaata atattcttaa aatgtggtaa tttattaaat ctgtaataaa agcgtaaaca
     6541 actgccgcta ggcttgctga tcccgcgcaa caaaacgcca tgctttgctc gcagatggtt
     6601 ggcaaccgac gacagtcctg ctaaaacgtt cgtttgatat catttttcct aaaattgaat
     6661 ggcagagaat catgagtgac agccagacgc tggtggtaaa actcggcacc agtgtgctaa
     6721 caggcggatc gcgccgtctg aaccgtgccc atatcgttga acttgttcgc cagtgcgcgc
     6781 agttacatgc cgccgggcat cggattgtta ttgtgacgtc gggcgcgatc gccgccggac
     6841 gtgagcactt gggttacccg gaactgccag cgactatcgc ctcgaaacaa ctgctggcag
     6901 cggtagggca gagtcgactg attcaactgt gggaacagct gttttcgatt tatggcattc
     6961 acgtcgggca aatgctgctg acccgtgctg atatggaaga ccgtgaacgc ttcctgaacg
     7021 cccgcgacac cctgcgtgcg ttgctcgata acaatatcgt tccggtaatc aatgagaacg
     7081 atgctgtcgc tacggcagag attaaagtcg gcgataacga taacctttct gcgctggcgg
     7141 cgattctggc gggtgccgat aaactgttgc tgctgaccga tcaaaaaggt ttgtataccg
     7201 ctgacccgcg cagcaatccg caggcagaac tgattaaaga tgtttacggc attgatgacg
     7261 cactgcgcgc gattgccggt gacagcgttt caggcctcgg aactggcggc atgagtacca
     7321 aattgcaggc cgctgacgtg gcttgccgtg cgggtatcga caccattatt gccgcgggca
     7381 gcaagccggg cgttattggt gatgtgatgg aaggcatttc cgtcggtacg ctgttccatg
     7441 cccaggcgac tccgcttgaa aaccgtaaac gctggatttt cggtgcgccg ccggcgggtg
     7501 aaatcacggt agatgaaggg gcaactgccg ccattctgga acgcggcagc tccctgttgc
     7561 cgaaaggcat taaaagcgtg actggcaatt tctcgcgtgg tgaagtcatc cgcatttgca
     7621 acctcgaagg tcgcgatatc gcccacggtg tcagtcgtta caacagcgat gcattacgcc
     7681 gtattgccgg acaccactcg caagaaattg atgcaatact gggatatgaa tacggcccgg
     7741 ttgccgttca ccgtgatgac atgattaccc gttaaggagc tggccgatgc tggaacaaat
     7801 gggcattgcc gcgaagcaag cctcgtataa attagcgcaa ctctccagcc gcgaaaaaaa
     7861 tcgcgtgctg gaaaaaatcg ccgatgaact ggaagcacaa agcgaaatca ttctcaacgc
     7921 taacgcccag gatgttgctg acgcgcgagc caatggcctt agcgaagcga tgcttgaccg
     7981 tctggcactg acgcccgcac ggctgaaagg cattgccgac gatgtacgtc aggtgtgcaa
     8041 cctcgccgat ccggtggggc aggtaatcga tggcggcgta ctggacagcg gcctgcgtct
     8101 tgagcgtcgt cgcgtaccgc tgggagttat tggcgtgatt tatgaagcgc gcccgaacgt
     8161 gacggttgat gtcgcttcgc tgtgcctgaa aaccggtaat gcggtgatcc tgcgcggtgg
     8221 caaagaaacg tgtcgcacta acgctgcaac ggtggcggtg attcaggacg ccctgaaatc
     8281 ctgcggctta ccggcgggtg ccgtgcaggc gattgataat cctgaccgtg cgctggtcag
     8341 tgaaatgctg cgtatggata aatacatcga catgctgatc ccgcgtggtg gcgctggttt
     8401 gcataaactg tgccgtgaac agtcgacaat cccggtgatc acaggtggta taggcgtatg
     8461 ccatatttac gttgatgaaa gtgtagagat cgctgaagca ttaaaagtga tcgtcaacgc
     8521 gaaaactcag cgtccgagca catgtaatac ggttgaaacg ttgctggtga ataaaaacat
     8581 cgccgatagc ttcctgcccg cattaagcaa acaaatggcg gaaagcggcg tgacattaca
     8641 cgcagatgca gctgcactgg cgcagttgca ggcaggccct gcgaaggtgg ttgctgttaa
     8701 agccgaagag tatgacgatg agtttctgtc attagatttg aacgtcaaaa tcgtcagcga
     8761 tcttgacgat gccatcgccc atattcgtga acacggcaca caacactccg atgcgatcct
     8821 gacctgcgat atgcgcaacg cccagcgttt tgttaacgaa gtggattcgt ccgctgttta
     8881 cgttaacgcc tctacgcgtt ttaccgacgg cggccagttt ggtctgggtg cggaagtggc
     8941 ggtaagcaca caaaaactcc acgcgcgtgg cccaatgggg ctggaagcac tgaccactta
     9001 caagtggatc ggcattggtg attacaccat tcgtgcgtaa ataaaaccgg gtgatgcaaa
     9061 agtagccatt tgattcacaa ggccattgac gcatcgcccg gttagtttta accttgtcca
     9121 ccgtgattca cgttcgtgaa catgtccttt cagggccgat atagctcagt tggtagagca
     9181 gcgcattcgt aatgcgaagg tcgtaggttc gactcctatt atcggcacca tttaaatcaa
     9241 taagttacac atcattagta cgttccttat ttcttgactg ggacaaattt gggaccgatg
     9301 ggttcagaat cgagtctatt tgccgtgcgt gttcggtaag gtgattaggt gcgaggtgag
     9361 catatcgacg aaccatttcg atagactccc agcctcccat ttcctgtaac actgataacg
     9421 ggactccggc ttgaaccagc caacttgccc aggtgtgtct caagtcgtga aatctgaaat
     9481 catcaatacc tgctcgtctc agcgccgctt tccaggctgt gtttgcgtca taccgcatct
     9541 tcctgactgt tggcgctttc gttccgtctg gtttggtaca gctttcctta tacacaaata
     9601 cccaacggtg atgattcccg atttgttttt tcaatacgcg acatgcagta tcattcagcg
     9661 caacgccaat tgcgcggttt gatttactct cttccgggtt tatccatgcc acccggcgct
     9721 gcatgtctat ttgttgccat tcaaggttga tgatgttcga gcgtcttaag cctgttgcaa
     9781 gtgcaaattc aacaacagac tttaatggct ccggacattc atcaatcagc ctttgtgctt
     9841 catgaggctc cagccagcgg atccgtttat tctttggttg aggcacttta ataattggtg
     9901 ccttatccag cattttccat tcacgctctg cggctcttag cagggccttt ataaatgaaa
     9961 gatgcgtagc cttcgttgca acggacgctg gttttggcgt gtattctgga acaggtttcc
    10021 ctttttttct gcatgcttct gccctgagtt tccagttttc ctcatgacgc cggttcgtca
    10081 ttttctgcat tgctgaataa atttttgatt cagtaatgtc tcttggttgc attcctgcga
    10141 aatgttgaag ccagaatccg atccggcttt tgtcatcgtc cagtgatttt ttatgtgtga
    10201 accgccccgg gtttcctgga gagtgtttta tctg
//
